LOCUS       DASQJS010000001        10325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1193, whole genome shotgun sequence.
ACCESSION   DASQJS010000001 DASQJS010000000
VERSION     DASQJS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2571
                     /locus_tag="VEW08_00005"
     CDS             <1..2571
                     /locus_tag="VEW08_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ39137.1"
                     /translation="SQHQIETLPQITRNFLEFADTIPGVVFEVGANGQTRISSGAQNR
                     NGVNLYIDGVGQKGYVRSGLTGQASDTQGNPFPQLAIGEYKVITSNYKAEYDQITSAA
                     ITALSRSGTNEFDGEAFGTYSADNFRAKTPAELDSGRKAESESKEYGFAFGGPIIQDH
                     LHFFVTYEGKRFVTPVSVLTAFAPADVASQLPADALAQLGPTSVDFEEDLIFAKLDWA
                     LSERDSMQLSAKIRDEVSSGDRTGLGRARSATVNTNNDDKRFELSWKHSADRWLNELQ
                     FTYEDAFYVPLPINTDVNGAAYTWFDGQDRTVLEADGPDPRAGQNKGQKGWAIGNFIT
                     FSDLSWGAGVHTIKAGAKYKDVDLTAADSIPGNPVFYYDVTAAGTAAIPWKSVFALPL
                     AGFDSVVTTNDKQLGLFIQDDWTVNDHLTLNLGIRWDLEKNESYLDFVTPQFLLDALN
                     TEVSPGVTYGQSLGLSADPNVAIDINDYISTGNNRKAQDDSFQPRLGFSYDLAGDQAH
                     VIFGGAGRSYDRTLYDYLQLEQTKFALASAEVRFNTTDHPCTLNGSTCVTWDPVYASD
                     PGALLSLLNGMAGEVNLINNDLEIPYSDQFSLGMRNRVGDWNTSVSVTHVLSKEGFVF
                     TMGNRYPNGDFWQNRSQPWGNAPPGLAGVLLVGDTGIETDSTQVLLSAEKPFTLESRW
                     GATFAYTFTDAEHNRDINEHYLFDESSIDIFPFITSNAAAEHRVVATGSFAAPWGLLL
                     AGKLTWSTPIPRNVISCYAGTATFPTGAPCNPFAVDVDGTTGYQAIDLQITKNFELGD
                     MGSMYLRIDALNVTNEHNEVDYIDTVGTDGIILGSRYAPFGNITGVPRTIRMSFGVKF
                     "
     gene            2624..4144
                     /locus_tag="VEW08_00010"
     CDS             2624..4144
                     /locus_tag="VEW08_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HYJ39138.1"
                     /translation="MTDDRLKSIVIVGGGTAGWMAAAGLAHVLKGSGCAITLVESDDI
                     GTVGVGEATVPHLRAFNDSLQIDEVEFVRAVRGTFKLGIQFVDWGRIGDSYIHGFGTI
                     GHDFRALPFFQYWLKLHLQGKAHALDDYSINTAAAPHGKFLSGATDVPANSPLSRVAY
                     AYHFDAGLYAAFLRRYAEARGVRRTEGEVVDVQLRAADGFIESVTLKSGARIAGELFI
                     DCSGFRGLLIEQALKTGYEQWTRWLPCDRAVAVPCEKTGPATPYTRSTARAAGWQWRI
                     PLQHRTGNGYVYSSAHTSDGDAAAALLTNLDGRALADPRVLKFVTGRRRKMWSRNCVA
                     LGLASGFLEPLESTSIYLIQSGIARLIQMLPDRHMNPVLQDRYNAEAAFEMERIRDFL
                     ILHYFATERRDTEFWKYCGTMSLPESLEHVIRLFRDSGRFYRNSDEMFALTSWVQVMV
                     GQHIVPRAYHPLVDLVAEPELLKLDESVRGVIASCVAAMPAHEQFIERCCKAPAAA"
     gene            4156..5649
                     /locus_tag="VEW08_00015"
     CDS             4156..5649
                     /locus_tag="VEW08_00015"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HYJ39139.1"
                     /translation="MRTRLTSFILLLAGALLVPACNTPAPKPRPAPALKAPAVPLKTL
                     DPLFDDVEKRTFNFFWETTEGATGLVPDRWPAAPFASIAAVGFALNAYAVGVERGYVT
                     RAQARARVLTTLRFLHAAPQGADPANNAGYQGFFYHFLNFGSGKRYGNSELSTVDTAL
                     LMAGVLFVGGYFDQDNADEAQIRKLADELYRRVNWPWSIEHQPDIRMAWSPEGQFQTH
                     DWRGYNEAMLVYILALGSPTFPAARQTWVAWTSDYYRSWGTLEGQTHLTFGPMFGHQF
                     SAIWIDFRGIKDEFMWPLGLDYFENSRRAVLSQRAYAIRNPLDWRGYGPNIWGLTASD
                     GPGDFELSYLGTTRRFRGYSARGVGLAGRPEEIFDDGTIAPTAVIGSLPFAPEIVVPA
                     THEMQKRYGQYIYGKYGFIDAFNPSFDFDVALKRGRRVRGVGWVASDYLGIDQGAIVS
                     MIENHRSELMWTVMKRNRYLRRGLTLAGFEGGWLDEPVAETDLSPRL"
     gene            complement(5633..7060)
                     /locus_tag="VEW08_00020"
     CDS             complement(5633..7060)
                     /locus_tag="VEW08_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HYJ39140.1"
                     /translation="MSRLVAVSNRISVPKRGAAPGGLAVGLLAAMQARRGLWFGWDGE
                     TVETPSDEPAMVRKDGVTYASIDFDQAEYKDFYLGFCNGTLWPLFHYFVDGFRYSDVQ
                     YEAYQRMNQRYARQLAPLLEPDDLIWVHDYHLFPLAQKLREAGATQPMGLFLHIPFPH
                     FEVLRVLPVYGELLQAMMAYDVLGFQTEPDRDAFRSAVASVWGEQVLTSNDTVTLGDR
                     RVVTGVFPIGVDVDVIQREAVESQSTDACKRMIAGLLGRRLMIGVDRLDYSKGLVERF
                     KAYERFLETHPENQNRVTFLQIAPLSRSDVRAYAEIRRDLEQTTGRTNGRFADTDWTP
                     IRYLNRNFPHDVLMGFMRSALIGIVTPVRDGMNLVAKEFVAAQDPADPGVLILSTMAG
                     AARELTTAVMVNPYDSRGMAHAIQQAFNMPLAERRERHQAMVDLLRKNSITAWHTSFV
                     DTLDSVAERVPKLARKAGWRIRAGG"
     gene            complement(7057..8835)
                     /locus_tag="VEW08_00025"
     CDS             complement(7057..8835)
                     /locus_tag="VEW08_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HYJ39141.1"
                     /translation="MNYGVIGNCQVAALIDEQARMVWACLPRPDGDPVFSALLQKEGG
                     DSQTGVFAIDMIDLTRTEQSYMRNSAIIETKMFDAHGGVLVVRDFAPRFRSRGRVFRP
                     MMFVRSVEPLAGRPTLRLRLRPSAGFGERCEPGITGSHHIRFAADGLHYRVTTDASLA
                     ALLENRPVVLEQALHFIIGPDETLQESPAALAREFVGATLSYWQEWVRTLAVPADWQE
                     AVIRSAITLKLCTYEDTGAVLAALTTSIPEAPNSTRNWDYRYCWLRDSYFVVQTLNRL
                     GATRTMEAYLHYIDHIIASSTADTLQPLYGITGDPRAVEKIATTLSGYRGMGPVRFGN
                     LAAEQVQHDVYGSVILAATQLFFDHRLVRTGDQVLLERLYRLGRRAVATFEEPDAGPW
                     EFRGRLQRHTFSAAISWAGCDRLGRIARRVGDHVAAREWGAHADRMRQEILKGAWSEE
                     RQCFTSAFGNRDLDATSLLLPELGLLPATDPRFLKTLERIGEELRTGDLLFRYKHADD
                     FGMPENAFTICAFWYVNALAAAGRVDEARERFSRLLERRNPLGLLSEDISPATGELWG
                     NFPQTYSMVGVIGSALRLSRSWEEIV"
     gene            complement(8832..9602)
                     /gene="otsB"
                     /locus_tag="VEW08_00030"
     CDS             complement(8832..9602)
                     /gene="otsB"
                     /locus_tag="VEW08_00030"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HYJ39142.1"
                     /translation="MMQLATREAGTEHAVPLPTTAWCLFLDVDGTLLELADHPGAVFV
                     DPELGELLARLRSAANGAVALVSGRTIADLDRLFNDPTLLVAGMHGCERRDANGELHV
                     APVALEQLSGVRAGLQRLAARHPGILLEDKGAGLALHFLKARELEHELRAEVSLLAAP
                     LVPAFALLDGHAVIEVKPAAHTKDSAVTAFMEEAPFRGRQPIFVGDDQTDYGGFAAVR
                     RFDGLAVAVGPRVKAEWWLPGPAAVRQWLGQLASAVSP"
     gene            complement(9725..>10325)
                     /locus_tag="VEW08_00035"
     CDS             complement(9725..>10325)
                     /locus_tag="VEW08_00035"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HYJ39143.1"
                     /translation="PFVTSSNTTGGFAGTGTGIGPRSFDYVLGIVKAYTTRVGAGPFP
                     TELFDEYGEHLSRVGHEFGAVTGRKRRCGWFDAVALRRSIIHSSVSGLCLTKLDVLDG
                     LDSLQICTGYRLSKDAGEGKISMEPPMYADAFADVEPVYEEMPGWKESTIGVTSYDAL
                     PLNARNYLARLQELAGVPIDIISTGPDRDQTIVLRHPFD"
BASE COUNT         1961 a         3409 c         3187 g         1768 t
ORIGIN      
        1 tcccagcacc agatcgagac cctgccgcag atcacccgca acttcctgga gttcgccgac
       61 accattccgg gcgtcgtatt cgaagtcggc gcaaatgggc aaaccaggat cagtagcggg
      121 gcgcagaaca ggaacggcgt gaacctctat atcgacggcg taggtcaaaa gggttacgtg
      181 cgttcgggcc tcactggcca ggccagcgac acgcagggca acccgttccc gcagctcgcc
      241 atcggtgaat acaaggtcat cacgtcgaac tacaaggcgg aatacgacca gatcacgagt
      301 gcggcgatca ccgcgctctc gcgctcgggc accaatgaat tcgacggcga agcgttcggc
      361 acgtattccg cggacaactt tcgcgcgaag acgccggccg agctcgactc agggcgcaag
      421 gccgagtcgg agagcaagga atacggcttt gcattcggtg gacccataat tcaggaccac
      481 ctgcatttct tcgtgaccta cgaaggcaaa cgcttcgtga ctccggtgag cgtgctgacg
      541 gcattcgcac cggccgacgt ggcctcacag ctgcccgccg atgcgctcgc gcaactcgga
      601 cccacgtctg tcgacttcga ggaagacctg atcttcgcga aacttgactg ggcactctcc
      661 gaacgcgaca gcatgcagct ctcggcaaaa atccgtgatg aggtcagctc cggcgatcgc
      721 actggcctgg gccgcgcacg cagcgcaacc gtgaacacga acaatgacga caaacgattc
      781 gagttgtcgt ggaagcacag cgccgaccgt tggctcaatg aattgcagtt cacttacgag
      841 gacgcgttct acgttcccct gcctatcaac accgacgtca acggtgcggc gtacacctgg
      901 ttcgacgggc aggaccggac cgtcctggag gcggacggcc ccgaccctcg tgcgggccag
      961 aacaagggtc agaagggctg ggccatcggc aatttcatca ctttttccga cctctcatgg
     1021 ggcgctggcg tccacaccat caaggctggc gcgaagtaca aggacgtgga cctgactgcc
     1081 gcggactcca ttcccggcaa ccccgtgttc tattacgacg tgacggcggc aggcacggcg
     1141 gccattccgt ggaagagcgt gtttgcattg ccactcgcgg gtttcgactc ggtagtgacc
     1201 accaatgaca agcagttagg cctgttcatc caggacgact ggaccgtcaa cgatcacctc
     1261 acgctcaacc tcggcatccg atgggacctc gagaagaacg agtcgtacct cgacttcgtg
     1321 acaccgcagt tcctgttgga tgcgctcaat accgaagtgt cgccgggagt cacatacggt
     1381 cagtcgctgg gcctgtccgc cgatccgaat gtggcaatcg acatcaatga ctacatcagc
     1441 acgggcaaca accgcaaggc gcaggatgac tctttccagc cacgtttagg tttctcctat
     1501 gacctggccg gcgaccaggc gcacgttatt ttcggcggcg cgggacgctc ctatgaccgc
     1561 acgctgtacg actacctgca gctcgaacag accaaattcg cgctcgcgag cgcggaagtc
     1621 cgcttcaaca ccacggatca tccctgcacc ctgaacggct cgacctgcgt gacctgggat
     1681 ccggtgtacg ccagcgaccc cggcgcgctg ctgtcgctgc tcaacggcat ggcgggcgaa
     1741 gtcaacctca tcaacaacga tctggagatc ccctactccg accagttcag cctcggcatg
     1801 cgcaaccgcg tcggggactg gaacacgagc gtgtcggtaa cacacgtcct cagcaaggag
     1861 gggttcgtat tcacgatggg caatcgctat ccgaacggtg acttctggca gaaccgctcg
     1921 cagccctggg gcaacgcgcc gcccgggctc gccggcgtat tgctggtggg cgacaccggc
     1981 atcgaaaccg acagcacgca ggtcctgttg tcagcggaaa aaccatttac gctggagtcg
     2041 cgttggggag cgacgttcgc ctacacgttc acggatgcgg agcacaatcg cgacatcaac
     2101 gagcactatc tattcgatga gtcgtcgatc gacatctttc cgttcatcac gtccaacgcc
     2161 gcggccgaac atcgcgtggt cgccacgggt tcattcgcgg cgccgtgggg actcctgctc
     2221 gcgggcaagc tgacctggtc cacgcccatt ccgcgcaacg tgatctcctg ttatgcgggc
     2281 acggccactt tcccgaccgg cgcaccgtgc aacccgttcg ccgtcgacgt ggacggaacc
     2341 acgggctatc aggcgatcga cctgcagatc accaagaact tcgagctcgg cgatatgggc
     2401 tcgatgtacc tgcgaatcga cgcgctcaac gtgaccaacg agcacaacga ggtcgactac
     2461 atcgataccg tcggcaccga cgggatcatc ctcggcagca gatacgcgcc gttcggcaac
     2521 atcaccggcg tgccgcggac gatacgcatg tcctttggcg tgaagttctg atgccaggaa
     2581 agctttgctg ttagaccccc cgaacggtgc cacaatcggc gcgatgaccg acgaccgact
     2641 gaaaagcatc gtcatcgtgg gaggtggcac cgccgggtgg atggcggcgg cggggctcgc
     2701 gcacgtgctc aaaggaagcg gctgcgcgat cacactcgtg gaatccgacg acatcggcac
     2761 ggtgggtgtg ggcgaagcca ccgtgccgca tctgcgcgcc ttcaacgact cgctgcagat
     2821 cgatgaagtc gaattcgtac gcgcggtgcg cggcacgttc aagctcggca tccagttcgt
     2881 ggattgggga cgcatcggcg actcctacat ccacggcttt ggcaccatag ggcatgactt
     2941 tcgtgcgctg ccctttttcc agtactggct gaagctccac cttcagggca aggcgcacgc
     3001 ccttgacgac tattcaatca ataccgccgc ggcaccgcac ggtaagttcc tgtccggcgc
     3061 caccgatgta ccggcaaact caccactgtc gcgcgtggcg tatgcctatc acttcgatgc
     3121 cggcctgtac gcggcattcc tgcgccgcta cgccgaggcg cgcggcgtgc ggcgcacaga
     3181 aggcgaagtc gtcgacgtgc agctgcgcgc cgccgacggt ttcatcgaat ccgtcacgct
     3241 gaagagcggc gctcgcattg cgggcgaact gttcatcgac tgctcggggt tccgcggtct
     3301 cctgatcgaa caggcgctga agaccggcta cgaacaatgg acgcgctggc tgccctgcga
     3361 tcgcgcggtg gccgtgccct gcgagaagac cgggcccgcg acaccgtaca cgcgctcgac
     3421 ggcgcgcgct gccggctggc agtggcgcat ccccttgcag caccgtacgg gcaatggcta
     3481 cgtgtattcg agcgcgcaca ccagcgatgg tgatgcagcc gccgcattgt tgaccaacct
     3541 cgacggccgc gcgctcgccg atccgcgcgt cctcaagttc gtgaccggcc gcaggaggaa
     3601 gatgtggtcg cgcaactgcg tcgcactcgg actcgcgagc ggcttcctcg aaccgctgga
     3661 gtccaccagc atctatctca ttcagtcggg aatcgcacgc ctgatccaga tgctgcccga
     3721 ccggcacatg aatccagttc tgcaggaccg ctacaacgcc gaggcggcgt tcgagatgga
     3781 acgcatccgt gatttcctga tcctgcacta cttcgcgacc gagaggcgcg acaccgagtt
     3841 ctggaaatat tgcggcacca tgtcgctgcc agagtctctc gagcacgtga tccgcctgtt
     3901 tcgcgacagc ggacgcttct atcgcaacag cgacgagatg tttgcgctca cgagctgggt
     3961 gcaggtcatg gtgggccagc acatcgtgcc gcgcgcctat catccgctgg tcgacctcgt
     4021 cgccgaaccc gaactattga agctcgacga gagcgtgcgc ggcgtgatcg cgagctgcgt
     4081 cgccgccatg ccggcgcacg agcaattcat cgagcgttgc tgcaaggcgc cggcggccgc
     4141 atgatgcgag gactcgtgcg cactcgcctc acaagcttca tccttctgct ggccggcgcg
     4201 ctgctggtgc cagcctgcaa tacgccggcg cccaaacccc gcccggcacc ggcgctcaaa
     4261 gcgcctgccg ttccgctgaa gacgctggac ccgctgttcg acgacgtcga gaagcgcacc
     4321 ttcaacttct tctgggaaac caccgaagga gccacgggcc tcgtgcccga tcgctggccg
     4381 gccgcaccgt tcgcgagcat cgccgccgtg ggcttcgcgc tgaatgccta tgccgtgggc
     4441 gtcgagcgcg gctacgtcac gcgcgcccag gcacgcgcgc gcgtgctgac gaccctgcga
     4501 ttcctgcacg cggcgccgca gggtgccgac cctgccaaca atgccggcta ccagggtttt
     4561 ttctatcact tcctcaattt cggcagcggc aagcgctatg gcaattccga actatccact
     4621 gtcgacaccg cactgctcat ggccggcgtg ttgttcgtcg gcggctattt cgaccaggac
     4681 aacgccgacg aagcgcagat ccgcaagctc gccgacgagc tctaccggcg cgtgaactgg
     4741 ccctggtcga tcgaacacca gcccgacatc cgcatggcct ggagtcccga ggggcaattt
     4801 cagacacacg actggcgcgg ctacaacgaa gccatgctgg tgtacatcct ggcgcttggt
     4861 tcgccgacgt ttcccgccgc ccgccagacc tgggtggcgt ggacgtccga ctactatcga
     4921 agctggggca cgctcgaagg gcaaacgcac ctgacgttcg gaccgatgtt cggccatcaa
     4981 ttcagcgcga tctggatcga cttccgcggc atcaaggacg aattcatgtg gccgctgggt
     5041 ctcgactatt tcgagaacag ccggcgcgcg gtgctctcac aacgcgcgta cgccatccgc
     5101 aatccactcg actggcgggg ttatggccct aacatctggg gcctcactgc ctccgatggc
     5161 cccggggatt tcgagttgtc ctacctcggc accacgcgca ggttccgcgg ctattccgca
     5221 cgaggcgtgg gactggcggg caggcccgag gaaatcttcg acgacggcac gatcgcaccc
     5281 acggccgtca tcggctcgtt gccgttcgcg cccgaaatcg tcgttccggc gacgcacgag
     5341 atgcagaagc gttacggcca gtacatctac ggcaagtatg gattcatcga cgcgttcaac
     5401 ccgagcttcg acttcgacgt cgcgctcaag cgcggccggc gcgtgcgcgg ggtcggctgg
     5461 gtggccagcg attacctggg catcgaccag ggcgccatcg tttcgatgat cgagaatcac
     5521 cgcagcgaac tcatgtggac cgtgatgaag cgcaatcgct acctgcgccg cggcctcacg
     5581 ctggcggggt tcgaaggcgg ctggctcgat gaacccgtcg ccgaaaccga tctcagcccc
     5641 cggctctgat cctccagccg gccttgcgcg ccagcttggg cacgcgctcg gcaacggaat
     5701 cgagcgtatc cacgaagctc gtgtgccacg ccgtgatcga attcttccgg agcaaatcga
     5761 ccatcgcctg gtggcgttcg cgcctttcgg caagcggcat gttgaaggcc tgctggatgg
     5821 catgcgccat gccgcgcgag tcgtagggat tcaccatgac cgcggtggtc agctcgcgtg
     5881 cagcaccggc catggtcgac aggatgagta cgcccggatc ggccggatcc tgcgcggcca
     5941 cgaattcctt cgccaccagg ttcatgccat cgcgcaccgg cgtgacgatg ccgatgagcg
     6001 ccgaacgcat gaaccccatg agcacgtcgt gtggaaaatt gcgattgaga taccggatgg
     6061 gggtccagtc ggtgtcggcg aagcgtccat tggtacggcc cgttgtctgt tcgagatcgc
     6121 ggcggatctc ggcataggcg cgtacgtcgc tgcgtgacag gggtgcgatc tgcaggaagg
     6181 tcacgcggtt ctgattctcg ggatgtgtct cgaggaagcg ctcgtaggcc ttgaaccttt
     6241 cgaccaggcc cttgctgtag tcgagccggt cgacgccgat catgaggcga cgccctaaca
     6301 acccggcgat catgcgtttg caggcatcgg tcgactggga ttccaccgcc tcgcgctgga
     6361 tcacgtccac gtcgacgccg atcgggaaca cgccggtcac gacacgcctg tcccccaagg
     6421 tcacggtgtc gttggaggtc aagacctgtt cgccccacac cgaagccacc gccgagcgga
     6481 acgcatcgcg atcgggctcc gtctgaaacc cgagcacgtc gtacgccatc atggcctgca
     6541 gcagttcgcc gtacaccggc aacacgcgca ggacctcgaa gtgcggaaac ggaatgtgca
     6601 ggaacagccc catgggctgg gtcgcaccgg cttcacgcag cttctgcgcc agcgggaaca
     6661 ggtgatagtc gtgcacccag atcaggtcgt cgggctcgag cagcggtgcc agctggcgcg
     6721 cataacgctg gttcatgcgc tgataggcct cgtattggac gtcgctgtaa cggaacccgt
     6781 cgacgaaata gtgaaagagc ggccacaacg tgccattgca gaagccgaga tagaaatcct
     6841 tgtactcggc ctggtcgaaa tcgatcgacg cataagtgac gccgtccttg cgcaccatcg
     6901 ccggctcgtc ggagggcgtc tcgacggtct cgccatccca gccaaaccac aggccgcgcc
     6961 gcgcctgcat ggcggctaac aaccccaccg cgagcccgcc aggcgcggcg cctcgcttcg
     7021 ggacggatat ccgattggaa actgcgacca gacggctcat acgatctcct cccagctgcg
     7081 cgatagtcgc aacgcactgc cgataacccc gaccatgctg taggtctgcg gaaaattccc
     7141 ccatagttca ccggtagccg gcgagatgtc ctcggagagc aagcccagcg gatttcgccg
     7201 ttcgagcaga cgtgaaaacc gctcgcgcgc ctcatccaca cggcccgcgg ccgcgagcgc
     7261 gttcacgtac cagaatgcgc agatggtgaa ggcgttctcc ggcatgccga aatcgtccgc
     7321 atgtttgtag cggaacagca ggtcgcccgt gcgcaactct tcgccgatac gctccagcgt
     7381 cttgagaaaa cgcggatcgg tcgccgggag caaccccagc tcgggcagca acagtgacgt
     7441 cgcatcgaga tcgcgattgc cgaaagccga agtgaagcac tgccgctctt cgctccaggc
     7501 gcctttcagg atttcctgcc tcatgcggtc ggcgtgtgcg ccccactcgc gcgccgcgac
     7561 gtggtcaccc acgcgccgcg cgatgcggcc gagccgatcg caacccgccc aggagatcgc
     7621 agccgaaaat gtgtggcgtt gcagtcgacc acggaactcc catggacccg cgtccggttc
     7681 ctcgaacgtc gcgaccgcgc gccggcccag ccggtagagc cgctcgagca gcacctggtc
     7741 gcccgtgcgc accagccggt gatcgaaaaa cagctgcgtg gcggccagga tcaccgagcc
     7801 gtacacgtcg tgttgaacct gctcggcggc gagattgccg aagcgcaccg ggcccatgcc
     7861 gcgataaccc gaaagtgtcg tggcaatttt ctccaccgca cgtggatcac cggtgatgcc
     7921 gtacagcggt tgcagcgtgt cggccgtcga cgaagcgatg atgtggtcga tgtagtgcag
     7981 ataggcttcc atggtgcgcg tcgcgccgag gcggttgagc gtctgcacca cgaaatagct
     8041 gtcgcgcagc cagcagtagc gatagtccca attgcgcgtg ctgttcggcg cctcgggaat
     8101 ggaagtggtc aacgccgcca gcaccgcgcc ggtgtcttca taggtgcaca gcttcagcgt
     8161 gatcgccgag cggatcaccg cttcctgcca gtccgccggc acggcgagcg tgcgcaccca
     8221 ctcctgccag tacgaaagcg tggcgccaac gaactcacgg gccagggccg caggggattc
     8281 ctgcagggtc tcgtccgggc cgatgatgaa atgcaatgcc tgttcgagca ccaccgggcg
     8341 gttctcgagc agagcggcca gtgatgcgtc ggtggttacg cgatagtgca ggccgtcagc
     8401 cgcgaaacgg atgtgatgac tgccggtgat gccgggctca caacgttcgc cgaaacccgc
     8461 ggaaggacgc agccgcagcc gcagagtcgg ccggccggcg agcggctcca cgctgcgcac
     8521 gaacatcatc ggcctgaaca cgcggccacg gctgcgaaac ctcggagcga aatcgcgcac
     8581 gaccaggacg ccaccgtgag catcgaacat cttcgtttcg atgatggcgg agttgcgcat
     8641 gtagctctgc tcggtgcgcg tgagatcgat catgtcgatc gcgaacacac cggtctggga
     8701 gtcaccgccc tccttctgca acagtgccga aaacaccgga tcgccatcgg gccgcggcag
     8761 gcaggcccac accatgcgtg cctgttcgtc gatgagcgcc gccacctggc aattgccgat
     8821 gacgccgtag ttcacgggct caccgcggac gcgagctgcc ccaaccactg tcgcacggcg
     8881 gccggccccg gtagccacca ttcggccttg acgcgtggtc cgaccgcgac ggccaggcca
     8941 tcgaagcggc gcacggcggc gaagccgcca tagtcggtct ggtcgtcgcc aacgaaaatg
     9001 ggctggcggc cacgaaacgg cgcctcttcc atgaacgcgg tcaccgcgct gtccttggtg
     9061 tgcgcggccg gcttcacttc gatgacggca tgtccatcca gcaaggcgaa tgccggcacc
     9121 agcggcgcag ccagcagcga cacctccgct cgcaattcgt gttcgagttc gcgcgccttc
     9181 aggaaatgca gcgccagccc cgcgcccttg tcttcgagca ggatgcccgg gtggcgcgca
     9241 gccaggcgct gcagcccggc gcgcactcca gacaattgtt cgagcgccac gggcgccacg
     9301 tgcaactcgc catttgcatc gcggcgctcg cagccgtgca tgccggccac gagcaaagtc
     9361 gggtcgttga acagccggtc gagatcagca atggtccggc cgctcaccag cgccacggcg
     9421 ccattggctg ccgagcgcag ccgcgcaagc agctctccga gctccggatc cacgaacacc
     9481 gctcccgggt gatccgcgag ctcgagcagg gttccatcca cgtcgagaaa cagacaccag
     9541 gcagtcgtgg gcagcggtac cgcatgttcg gtgcccgctt cgcgcgtcgc gagttgcatc
     9601 atccgcgcag tatatgaatg ccggcgggca atggcccgcg gaattccggt agctaggcgt
     9661 gagagttttt tcgcaagcgg cgcgcagcgg cgattgccgt cggacccgtc ctacttcggc
     9721 ccgctcagtc gaatggatgc cgcagcacga tggtctgatc gcgatccggg cccgtcgaaa
     9781 tgatgtcgat gggcacgccg gcgagttcct ggagccgcgc gaggtagttg cgtgcattca
     9841 ggggcaaggc atcgtacgac gtgacgccga tggtggattc cttccagccc ggcatctctt
     9901 catacaccgg ctccacgtcc gcgaacgcat cggcgtacat gggcggctcc atgctgatct
     9961 tgccttcgcc cgcatctttc gaaaggcgat aaccggtgca gatctgcagc gagtcgagtc
    10021 cgtcgagcac gtcgagcttg gtcaggcaca ggcctgacac gctcgaatgg atgatggagc
    10081 ggcgcaacgc caccgcatcg aaccagccgc agcggcgctt gcgccccgtg accgcgccga
    10141 actcatgccc gacccgcgag aggtgctcgc cgtattcgtc gaacaactcg gtggggaacg
    10201 ggcccgcgcc cacgcgcgtg gtgtaggcct tcacgatgcc aagcacgtag tcgaacgagc
    10261 gcggaccgat gccggtgccg gttcccgcga acccgccggt ggtattcgac gaagtgacga
    10321 atgga
//
LOCUS       DASQJS010000002         4873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1257895, whole genome shotgun sequence.
ACCESSION   DASQJS010000002 DASQJS010000000
VERSION     DASQJS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257895"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="VEW08_00040"
     CDS             <1..1482
                     /locus_tag="VEW08_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ39144.1"
                     /translation="SERLQKVEYDLVLLDLGLPDAQGLQALTALRAVADLTPIIVLTG
                     SDDYKQGLSAVREGAQDYLEKRRVNAGMLSRIVSYSVERNNFHRDLVRATRRYQDLFN
                     NVPVALFTANQDGALKTANAACVKLLSCKGIPLDKVNFLDLLFEPGKRAQFLDQIAKQ
                     RAVEHWETTIQDCKGGKLNVLINTAPLYDAAQAFAGWEGCLTDISMLKQTLEERDRLE
                     DNLRQAQKLEAIGQLAAGIAHEINTPTQYVGDNLRFLKESFGELDTLLGQLVEVGGGP
                     ASKIIGEADFEYLKEEIPRALNQSLEGVDRVAKIVRAMKEFSHPAREKTATDLNRAIQ
                     STITVASNEWKYVAEIEMDLDANLPAVHCSPAEFNQVVLNIIVNAAHAISDVVGDGGK
                     GKGKIGVRTRADGDFAIIEISDTGSGMPPHVQQRIFDPFFTTKEVGKGTGQGLAIAHN
                     VIVDKHSGTIKVTSSPGAGTTFTIRLPIGGAKADMETAAGAAA"
     gene            1479..2693
                     /locus_tag="VEW08_00045"
     CDS             1479..2693
                     /locus_tag="VEW08_00045"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ39145.1"
                     /translation="MNAIRVIFVDDEPRVLEGLKRMLRPKRNEWQMTFVGSAQAALDA
                     LRAEPCEVVVTDMRMPGMNGAELLEVVQREYPNTIRLILSGQAETESVMKALGVSHQF
                     LSKPCDADILQGTISRAFALRDLAGSDSVKALVARINKLPALPATYQKLIEALRQPNS
                     SLEDVSRVISTDPSMTARLLKLVNSAYFGLQKPVADVSRAGALLGLDRIMALVLGQGI
                     FSDCEPPVVEGFSMDELWQHSMSTAAAAHRIAIAEHLDRDLVAAAFLAGMLHDIGKLV
                     LAMGMPEQYAKVLKQAGDRPGDLQQIEMLELHAAHSDVGAYLVGLWGLPNTIAEAIAY
                     HEDPSQSPNPKFGLAGIVHCADRLAHRPDIADVRSADLGLNFEYLEAQGRTGHWSEWR
                     EAYATVTRKATE"
     gene            2705..3286
                     /locus_tag="VEW08_00050"
     CDS             2705..3286
                     /locus_tag="VEW08_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ39146.1"
                     /translation="MDKSKLLLVDDEPNLTSALVRSLDRSQFEIFTADSAQKGLLILA
                     GNEIDVVVSDERMPGMTGSAFLAEVRKQWPNTIRMILSGQADLEAAVRAINEGEVYRF
                     LLKPCHPKELQMTILQGLQQKKLVAQSRKLLQEHQKTQNLLEELEKASPGITKIEMDE
                     DGAIMMDDSEGGADDVQSLLDDLEREMAKVARR"
     gene            complement(3397..4371)
                     /locus_tag="VEW08_00055"
     CDS             complement(3397..4371)
                     /locus_tag="VEW08_00055"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HYJ39147.1"
                     /translation="MYSRIAGTGSYLPEQIVTNFDLEKKMDTTDEWIRSRTGIERRHI
                     AADGQTTVDIGEIAARRAIEAAGVTPAEVDFIVFGTTTPDLVFPNCGTLLQARLGARG
                     CPAFSVETACSGFIYALSIADKFVKAGEAKCALVVGAETLSRITNWSDRNTAVLFGDG
                     AGAVVLKPSTEAGIMSTHLHSDGAYKDLLYVDSGVSKGFNVDPVTNKVDIGIKMAGSE
                     VFKVAVTKLGGAVDEALAHNGLEKSAVNWLIPHQANIRIIGAMAKRLDLPMERVIVTV
                     QDHGNTSTASVPLALDTGVRDGRVKRGDLLLLEAFGGGFTWGSALVRF"
     gene            complement(4394..>4873)
                     /locus_tag="VEW08_00060"
     CDS             complement(4394..>4873)
                     /locus_tag="VEW08_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39148.1"
                     /translation="EDTKGHELIQEAHELIRASGVNYLGFVEGNHIFDENVDVVVTDG
                     FTGNVALKAMEGLAKLIVEQMRTEFSRNLTSKLSGLLARPVLRRVGDKLDPRRYNGAC
                     MVGLTAVVVKSHGSADSVAFAQAISTAAIAMRRGLPAAIANALAKAATSAEATIGDA"
BASE COUNT          942 a         1590 c         1513 g          828 t
ORIGIN      
        1 tccgagcggc tgcagaaggt cgagtacgac ctggtgctgc tcgatctcgg cctgcccgac
       61 gcgcaaggac tgcaggcgct gaccgccttg cgggccgtcg cggatctcac gccgatcatc
      121 gtgctgaccg gatccgacga ctacaagcag ggtctctccg ccgttcgcga aggcgcgcag
      181 gactatctcg agaagcgccg cgtgaacgcc ggcatgctct cgcgcatcgt ttcgtattcg
      241 gtggagcgca acaatttcca tcgtgacctg gtgcgcgcca cgcgccgcta ccaggatctt
      301 ttcaacaacg tgccggtggc gctgttcacc gccaaccagg acggcgcgct caagaccgcg
      361 aacgcggcct gcgtgaagct gctttcctgc aagggcattc cgctcgacaa ggtcaacttc
      421 ctcgacctgc tgttcgaacc cggcaagcgc gcgcaattcc tcgaccagat cgccaagcag
      481 cgcgcggtgg agcattggga gacgacgatc caggactgca agggcggcaa gctcaacgtc
      541 ctcatcaaca cggcgccgct gtacgacgcg gcccaggcgt tcgccggctg ggaaggctgc
      601 ctcaccgaca tctcgatgct caagcagacg ctcgaagaac gcgatcggct cgaggacaac
      661 ctgcgccagg cgcagaagct cgaagccatt ggccagctgg ccgccggcat cgcgcatgaa
      721 atcaacacgc cgacgcagta cgtgggcgac aacctgcgct tcctcaagga gtcgttcggc
      781 gagctcgaca cgctgctcgg ccagctggtc gaggtcggcg gcggcccggc gagcaagatc
      841 atcggcgaag cggatttcga atatctgaaa gaagagatcc cgcgcgcgct caaccagtcg
      901 ctcgaaggcg tcgaccgcgt cgcaaagatc gtgcgcgcca tgaaggaatt ctcgcacccg
      961 gcacgcgaga agaccgccac agacctcaat cgcgcgatcc aaagcactat tactgttgcc
     1021 agcaacgagt ggaaatacgt cgccgagatc gaaatggatc tcgacgccaa cctgcccgcg
     1081 gtgcattgct cgcccgccga gttcaaccag gtggtgctca acatcatcgt gaacgccgcg
     1141 catgccatct ccgacgtggt cggtgacggc ggcaagggca agggcaagat cggcgtccgt
     1201 acgcgcgccg acggtgattt cgccatcatc gagatcagcg atacgggttc cggaatgccg
     1261 ccgcacgtgc agcagcgcat cttcgatccg ttcttcacca ccaaggaagt cggcaagggc
     1321 acgggccagg gcctggccat cgctcacaac gtgatcgtcg acaagcacag cggcacgatc
     1381 aaggtaacga gcagccccgg cgccggcacc acgttcacca tccgcctgcc catcggcgga
     1441 gccaaggcag acatggagac agcggctgga gccgctgcgt gaacgcgatc cgcgtcatat
     1501 tcgtcgacga cgaaccacgc gtgctcgagg gactgaagcg gatgcttcgg cccaagcgca
     1561 acgagtggca gatgacgttc gtcggcagtg cgcaggccgc gctcgacgcg ttgcgcgccg
     1621 agccttgtga agtcgtggtc accgacatgc gcatgcccgg catgaacggc gctgagctcc
     1681 tggaagtcgt tcaaagggaa tacccgaaca ccatccgcct gatcctctcg ggacaggcgg
     1741 aaaccgaatc ggtgatgaag gcgctcggcg tctcgcacca gtttctctcg aagccctgcg
     1801 acgcggacat cctgcagggg accatcagcc gcgccttcgc gctgcgcgac ctcgccggca
     1861 gcgattcggt caaggcgctg gtggcgcgca tcaacaaact accggccctg ccggcgacct
     1921 accagaaact catcgaggcg ctgcgccagc ccaactcgag cctggaagac gtctcgcgcg
     1981 tcatttcgac cgacccgtcg atgaccgccc gcctgctcaa gctggtgaat tcggcgtact
     2041 tcggcctgca gaagcccgtc gccgacgtat cacgcgccgg cgccctgctg ggcctcgacc
     2101 gcatcatggc gctggtgctc ggccagggaa ttttttcgga ctgcgagccg ccggtagtcg
     2161 aaggcttcag catggacgag ttgtggcagc acagcatgtc caccgccgcc gctgcgcatc
     2221 gcatcgccat tgccgaacat ctcgatcgcg acctagtcgc cgcggcgttt ctcgccggta
     2281 tgttgcacga catcggcaag ctcgtgctcg ccatggggat gcccgagcag tatgcgaagg
     2341 tgctcaagca ggccggcgat cggcctggag acctgcaaca gatcgaaatg ctcgagctgc
     2401 acgccgcgca cagcgacgtg ggcgcctatc tcgtcggcct gtggggcctg ccgaacacga
     2461 ttgccgaagc catcgcctat cacgaagatc cgtcgcagtc acccaacccc aagttcggcc
     2521 tcgccggcat cgtgcactgc gcggatcgcc tggcgcaccg cccggacatc gcggacgtgc
     2581 gttcggcaga cctcggtttg aatttcgaat acctcgaagc ccagggacgc acgggtcact
     2641 ggagcgaatg gcgcgaggcg tatgccacgg ttaccaggaa agcgactgaa taagggtaaa
     2701 ttttatggac aagagcaaac tactgctggt cgacgacgag ccgaacctca cctcggcgct
     2761 ggttcgcagc ctcgatcgct cgcaattcga gatcttcacc gccgactcgg cgcagaaagg
     2821 tctgctgatt ctcgcgggca atgagatcga cgtggtcgtt tccgacgaac gcatgccagg
     2881 catgaccggc agcgcgttcc tcgcggaagt gcgcaagcag tggccgaaca ccattcgcat
     2941 gatcctgtcg ggacaggcgg acctcgaagc ggcggtgcgc gccatcaacg agggcgaggt
     3001 gtaccgcttc cttctcaagc cctgccatcc aaaagaactg cagatgacca tcctgcaggg
     3061 acttcagcag aagaagctcg tggcccagag ccgcaagctg ctgcaggaac atcagaaaac
     3121 gcagaacctg ctcgaagaac tcgagaaggc cagcccgggc atcaccaaga tcgagatgga
     3181 cgaagacggc gccatcatga tggacgacag cgagggcggt gccgacgacg ttcagtcatt
     3241 gctcgacgac ctcgagcgcg agatggcgaa ggtggcgcgc cgctagacgg ttggtgccgg
     3301 gtgaggaaag tggggagtcg ccccacgtaa atcgtcaccc gcgtgactcg ttagcgactc
     3361 cccactttcc tcacccggca ccactcatca aggcacctag aatcggacga gcgccgagcc
     3421 ccaggtaaag ccgccgccaa aggcttccag cagcagcaag tcgccgcgct tcacgcggcc
     3481 gtcccgcacg ccggtatcca gtgccagcgg caccgaagcc gtcgacgtgt tgccatgatc
     3541 ctgcacggtg acgatgaccc gttccatggg caggtcgagc ctcttggcca tggcgccgat
     3601 gatgcggatg ttcgcctggt ggggaatgag ccagttgacc gccgatttct ccaggccgtt
     3661 gtgcgccagc gcttcgtcga cggcgccacc cagcttggtc acggccacct tgaacacttc
     3721 gctgccggcc atcttgatgc cgatgtcgac cttgttggtc accgggtcga cgttgaaacc
     3781 cttggacaca ccactgtcga cgtacagcag gtccttgtag gcgccgtcag agtgcaggtg
     3841 cgtgctcatg atgcccgcct cggtcgaagg cttgagcacc accgcgcccg cgccgtcgcc
     3901 aaacagcacg gcggtatttc gatctgacca gttggtgatg cgcgagagcg tctcggcgcc
     3961 gaccacgagt gcgcacttgg cctcgcccgc cttcacgaac ttgtcggcga tggacaacgc
     4021 gtagatgaaa ccgctgcacg cagtctccac cgagaacgcg ggacagccac gtgcgcccag
     4081 gcgcgcctgc agcaacgtgc cgcaattggg gaacacgagg tccggcgtgg tggtgccgaa
     4141 cacgatgaag tcgacttcgg ccggcgtgac ccccgcggcc tcgatggcgc gacgcgcggc
     4201 aatttcgccg atgtcgacgg tagtttggcc gtcggccgcg atgtgccgcc gttcgatgcc
     4261 ggtgcgcgag cggatccact cgtcggtggt atccatcttc ttctcgagat cgaagttggt
     4321 gacgatctgt tccggaagat aagagcccgt accggctata cgtgaataca tgctggccct
     4381 ttgtcctttg agactatgcg tcgccgatcg tcgcttcggc ggatgtcgcc gccttcgcca
     4441 gtgcattcgc aatggccgcc ggcagcccac ggcgcatcgc gatcgcggcc gtgctgatgg
     4501 cctgggcgaa tgcgacgctg tccgcgctgc cgtggctttt taccacgact gcggtcagtc
     4561 ccaccatgca ggcgccgttg tagcgccgcg gatcgagttt gtcgccgacg cgccgaagca
     4621 ccgggcgggc tagcaacccg gagagcttgc tggtcaggtt gcgcgagaat tccgtgcgca
     4681 tctgttcgac gatgagcttg gcgagacctt ccatcgcctt gagcgcgacg ttgccggtga
     4741 agccgtcggt gaccaccacg tcgacattct cgtcgaagat gtgattgcct tcgacgaagc
     4801 cgagatagtt cacgccgctg gcgcggatca gctcgtgcgc ctcctggatc agctcgtgtc
     4861 ccttggtgtc ttc
//
LOCUS       DASQJS010000003        15723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2202409, whole genome shotgun sequence.
ACCESSION   DASQJS010000003 DASQJS010000000
VERSION     DASQJS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15723
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202409"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VEW08_00065"
     CDS             <1..747
                     /locus_tag="VEW08_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HYJ39149.1"
                     /translation="ALDAARAAAEVIRGFYQRNVKIEVKADKTPVTEADVRSEEAIRD
                     LLTKRFPSYGFYGEETGKSDMNAESVWLVDPIDGTKSFVRECPFFSTQIALMRGGRFV
                     LGVSMAPAYDELAWAERGKGAFLNGKRISVSKVNTLEAAIVSSGNLKTLAASPAWARF
                     GGLIGRVNRIRGYGDFVHYHLLARSALDVVIESDVNILDIAALTVIVEEAGGRFTDLK
                     GSAVNLDTTTVLATNGALHETILENVRFGS"
     gene            750..1157
                     /locus_tag="VEW08_00070"
     CDS             750..1157
                     /locus_tag="VEW08_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39150.1"
                     /translation="MSVFLRWGIFGILGVAALLYAYNTSKSLAEKRSSREPPVAANAA
                     TPVEPEPVPTTAPHCQAELEVAQRALAARREGEPLDRLLRIQEIAWQEPVTRRHRLEE
                     VATRWYQLEGEEPIAEALRIRVISDCEHFSPAP"
     gene            complement(1141..1905)
                     /locus_tag="VEW08_00075"
     CDS             complement(1141..1905)
                     /locus_tag="VEW08_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ39151.1"
                     /translation="MSTVLEAAQLRIAVPGRTLIDSFDCTIRAGEFIALLGGNGTGKS
                     LLLRTLAGLRAPTAGLVRIDDRDITTVPRREVAMRLGFLPQDPEAAPQGSLRESALLG
                     RFAHLGFWEAAGAADTQRVARALADVGLEAFAERELGTLSGGEQRRASIARLLVQAPS
                     IYLLDEPTNHLDPAQQIGILDKLRHLTREGAAVIASLHEPNLALRYADRACLLSGNGQ
                     SELVDGAALGTDHLQRLYGIRYVETRLGQQRFMAPG"
     gene            complement(1902..2675)
                     /locus_tag="VEW08_00080"
     CDS             complement(1902..2675)
                     /locus_tag="VEW08_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39152.1"
                     /translation="MWRNVRIAFLLLILGVAAWSNWYDRLSTTDWDETLYVGVFPVEE
                     PGNPVVHDYIERLSSDHIADIEKFLNEEAREFGIGIARPVTVDLYPPVAERPPERGAS
                     SNVLQNMWWSLKLRMYARRAARESGKPPPQIRLFVLYHDPAFSQAVPHSVGLQKGLVG
                     VVHAFAAAGMTRGNNVVIAHEILHTLGASDKYDPDTLAPLFPIGYAEPDRTPRFPQPF
                     AEIMAGRSAVDARTFEIPESLDKVVVGEATALEIRWIGK"
     gene            complement(2677..3363)
                     /gene="pdxH"
                     /locus_tag="VEW08_00085"
     CDS             complement(2677..3363)
                     /gene="pdxH"
                     /locus_tag="VEW08_00085"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HYJ39153.1"
                     /translation="MELFNDLLPDPLPAEPMALASAWLATAIAARQQPNPDAMVLATA
                     TATGLPSARIVLCKEFDVEQGRVSFVTNYDSRKGRDLAENSRAALVFHWDHSHRQIRV
                     EGIVVKAPASESDAYFASRNWQRRIGAWASAQSQPLRGREQLTAAVLAAAKRFGVPEP
                     TLDNINVPVAADIPRPPFWGGYYVWADSVELWVEGASRIHERARWTRSLTTTADAFRT
                     GPWSATRLQP"
     gene            3466..4578
                     /locus_tag="VEW08_00090"
     CDS             3466..4578
                     /locus_tag="VEW08_00090"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HYJ39154.1"
                     /translation="MSSLEDLRRRINDLDRQILELVGKRQETSREVARAKRATGHATR
                     DYNREREVLLAAREAGKQYGVSPQVAENILRLLIRSSLTTQEQASVAAQGAGNGRTAL
                     VIGGAGKMGRWFAEFMASQGFAVEIADPAGAPPGFAHLPDWRTSPLGQDFIVIATPLG
                     VTDKILREVAMRRPKGVIFDVGSLKSPLRAGLNALKSHGCRVTSLHPMFGPNTELLSG
                     RHVIFVEAGSSEATSAARELFASTMVEQVVMSLDEHDRLIAYVLGLSHALNIAFFTAL
                     AESGEAAPRLAKLSSTTFDSQLDVASLVAQESPELYFEIQSLNDYGSESLEALSQAVE
                     RLRTAVISQDFEAFANLMRRGREYLEDRRSVAERRA"
     gene            4581..5690
                     /locus_tag="VEW08_00095"
     CDS             4581..5690
                     /locus_tag="VEW08_00095"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HYJ39155.1"
                     /translation="MSSRDPDSAPADELREHLRVLTEEVARNELLLRKTQAREIELLR
                     ANSLGELFDRIVTGLRDAYALDLVTLSLFDPQHELRHLSGNENLTTAVIEEVRFIDAV
                     SPLAPQLAQFDRPWLGRYSAVDHELLVSPAMKQGSVALIPLPRDSRAIGVIAFCSRDP
                     HRFTSDLAADFLAHLGVIVAISLENAVNRARLVRSGVTDFLTGWHNRRYFHNRLREEL
                     ARSERTGKTLACLMIDLDNFKDINDRFGHLAGDEALKEVARRAEAEIRAGDTGARFGG
                     DEFAILLAGAEIAHALKLAERIHHSVGSTPVRVHGGAHVTVTLSVGLATARPESRSHD
                     YKALAERLIAEADAALYRAKSAGRNRVEMSTHVIG"
     gene            complement(5694..6680)
                     /locus_tag="VEW08_00100"
     CDS             complement(5694..6680)
                     /locus_tag="VEW08_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HYJ39156.1"
                     /translation="MTSRPPTTALLVLLVALAAIVTCLGLVSGSTDIGVRPAIAALFG
                     DPAEPARTVVMELRAPRLLSAFTIGALLALAGVLLQALFRNPLADSYVLGVSGGSSVG
                     ALFALLLGASLWAVQCSAAFGAIAATFLVLALARGGATVRVLLTGVVVASACGALVTL
                     VLVLADNGQLRGMMFWLAGDLGWAAHPWVMLAAALLATLAVTLIARPLDVLSTGEITA
                     QSLGLDLPRWRMVIVVFAGLLTAGAVIQGGTIGFIGLVIPHLVRLAFGTAAHRVVAPA
                     AALAGGTLLAAADLVARTAAAPRQLPVGAIMALLGVPMFLVLLRRLEGPRST"
     gene            6759..7307
                     /locus_tag="VEW08_00105"
     CDS             6759..7307
                     /locus_tag="VEW08_00105"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016144233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HYJ39157.1"
                     /translation="MSDWIDSQGYRANVGIVLMRDSGEVFLGQRTGARGWQFPQGGVR
                     QGEPVEHALFRELEEEIGLQPQHVTLMGSTADWIRYRLPKRYVRRGRGPTCIGQKQRW
                     FLLRLAVPEDAAEFSFTKTSEPEFDGWRWANYWEPVREVIYFKRPVYVRMLTELAPTA
                     FPAGPPPLPEWWQSETAATVEE"
     gene            7381..8040
                     /locus_tag="VEW08_00110"
     CDS             7381..8040
                     /locus_tag="VEW08_00110"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HYJ39158.1"
                     /translation="MVLVFIVAAGVYGMYEFGRYRAGYDVVAGLKQRTEMRNEIATLE
                     ATIFDLRAKVAQLESSTVGQTREREEVQRTIGDLQAQVARANQELAFFRGIVTQNANS
                     AEVKIQQARMVATATANKFRVRVTLVQPMKPDTVVTGVVVLSVDGEVDGKPGRADFAT
                     LTGGKRREIAFTFRYLENIDEEITMPPGMKPEQLLVEVRSNRRGSAPVQQSYVWSVDP
                     Q"
     gene            8063..8485
                     /locus_tag="VEW08_00115"
     CDS             8063..8485
                     /locus_tag="VEW08_00115"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HYJ39159.1"
                     /translation="MFGRRKQSSTRIDTLLGKTATLNGDLEFSGGLHLDGRVNGNVSS
                     NATDGGALSVSESGFIEGNVEVTNIVMNGTVNGDMHARDRLVLGGSARVNGNVHYGVI
                     EMAPGAVITGKLIPLGKDKAPETQPEAAPPVAAAKSGA"
     gene            8496..8840
                     /gene="erpA"
                     /locus_tag="VEW08_00120"
     CDS             8496..8840
                     /gene="erpA"
                     /locus_tag="VEW08_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005244817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HYJ39160.1"
                     /translation="MSETETETLKFTDAAARKVGDLIRGEGNPNLKLRVFVQGGGCSG
                     MQYGFEFDEQEQDGDTCVENLGVKLLIDPMSVQYLTGAEIDYREGLDGAQFVIRNPNA
                     QTTCGCGSSFSA"
     gene            8857..10374
                     /locus_tag="VEW08_00125"
     CDS             8857..10374
                     /locus_tag="VEW08_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HYJ39161.1"
                     /translation="MARRAPKSVPDVLAAVDLGSNSFHMVVARYTHGQLVIVDRLREM
                     VRLGAGLEADGRLNKDVAARALACLERFGQRLRDMGPGSVRVVGTNVFRRTRKKEAFI
                     ERAREAIGHPIEIVSGIEEARLIYSGVAHTMAAGAGRRLVCDIGGGSTEIIIGEGLEP
                     LELESLQIGCVRLSEEFFAEGRLSQKRMQRARVAARQAIEPYQAAFRRRGFDEAVGSS
                     GTVRAIGDAIRELDPAAVDIGADGLERIINELGGLEHIRDLKLSAITEDRKHSFAGGV
                     AIMAEVFEQLGIETMRVADGAMREGLLYDLLGRYTDEDARERTVRSMQQRYHVDLVQA
                     ERVEATAVEFLSQVEDSWSLADPAIEQLLRWAARLHEIGLDVAHSGYHRHGAYLLANA
                     DMAGFPREEQKLLAIMVGSHRRKPAIEEKDDLAAPWDKRAPAMTLLLRLAVLLHRGRS
                     SVALPAIKLGARADSLEMRFPPDWLDDHPLTVADLQQEVENLKSVAFRLRAFTAK"
     gene            complement(10383..12467)
                     /gene="ppk1"
                     /locus_tag="VEW08_00130"
     CDS             complement(10383..12467)
                     /gene="ppk1"
                     /locus_tag="VEW08_00130"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HYJ39162.1"
                     /translation="MDQPNLHSPEHYINRELSLLEFNGRVLAQALDEKVPLLERLRFL
                     CISSNNLDEFFEIRVAGLKQRLELGSDQPGADGLTVTEQLQAIHERTRRLVDAQYACL
                     NEVVLPGMRRQGIELLARTTWDSATAQWLERYFELEVEPVLSPLALDPARPFPRIQNK
                     SLNFIVRVAGQDAFGRDSGLAIVQAPRSLPRVVALPEANGLKRFVLLTGIVQQFVSRL
                     FSGMEVLSCHQFRVTRNSDLFVDDEEVDDLRRALEGELAHRRYGAAVRLEVSKDCPPD
                     LNAFLMRQFALNEVDFYEVSGPVNLNRLDAIYDLAQRPDLKYPIFTPGLPRRVASSTD
                     LLAVIRQKDVLLHHPFHSFGPVLDFLRGAATDPRVLAIKQTLYRTGNESPIVDALVSA
                     AQAGKDVTVIVELRARFDEEANIELSSRLQEAGAHVMYGVVGFKTHAKMSMVVRRESD
                     GLRRYCHLGTGNYHPRTARGYTDYSLFTCDPTIGQDVHEMFLQLTSLTQTPRLTKLLQ
                     SPFGLHEKVVEKIEREAQHAAAGKPARILAKMNSLVDPQVIEALYRASRAGVQIDLII
                     RGLCSLRPGIPAVSENIRVRSIVGRFLEHSRVFFFGNDGASELYLASADWMERNFFRR
                     VEIAFPVREETHRDRIVRDLNSYLSDNTRAWVLGRDGHYSRCVRGDDPARDAQGALLA
                     RYAAGSAPAVLP"
     gene            complement(12533..13834)
                     /locus_tag="VEW08_00135"
     CDS             complement(12533..13834)
                     /locus_tag="VEW08_00135"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYJ39163.1"
                     /translation="MPIFIKSTIPGLPTRTAYLLQIGLVAIVAALVALPRDREMPPLA
                     AQPSAEAPATPASQSPPGTHEASLGFSTIEVVVSRNDTMDQLFRRFELNLGDLANLRN
                     LPELRSQVDRLKPGELLRFMHRGGELVGLERKLSDSETLKVTRDANGFSSDVLENPLE
                     VRTRTASATIQNSLFQAAADAELSDRVAFELAEIFQYDIDFVLDIQSGDRFTVVYEEV
                     FQDGVPLRIGNILAAKFVNDGREYRAVRFVDVDGRGQYFSPDGKSLRKAFIRAPVQFS
                     RISSRFNPSRRHPVLNRIRAHRGVDYAAPVGTPVRAAGEGRVRFVGQQGGYGNVIELE
                     HGSGVVTVYGHLSRFATKLRRGQHVDLAQVIGYVGKTGLATGPHLHYEYRIRGVHKNP
                     QTVQLPDAEPIPNAERERFLNDTALLVNMLDLPAGPALVAR"
     gene            13953..15173
                     /gene="tyrS"
                     /locus_tag="VEW08_00140"
     CDS             13953..15173
                     /gene="tyrS"
                     /locus_tag="VEW08_00140"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HYJ39164.1"
                     /translation="MDGELQNQMAELLRGTQEVLVESELVKKLIRGKPLRIKAGFDPT
                     APDLHLGHTVLINKMRQFQNFGHEVTFLIGDFTGMIGDPTGRNATRPPLTREQVDANA
                     QTYQEQIFKILDRDKTRVDFNSRWFGGMTAAGLIEIAGRHTVARMLERDDFAKRYKGN
                     QPIAIHEFLYPLVQGYDSVALKSDVELGGTDQKFNLLVGRQLQESYGQEPQIVMTMPL
                     LEGLDGVNKMSKSLGNYIGINESADIQFGKIMSISDDLMWRYFELLSFRPLADVAALH
                     KAVLEGRNPRDVKFELGVEIVDRFHGAGAGVRSREAFVARFQQGQVPEQIESISLTTD
                     GSPQRLANVLKSAGLVASTSDGNRMIDQGAVRIGDSAETLSKVAGRDHVLGPGKFLVQ
                     VGKHKFRWVTLQSG"
     gene            15592..>15723
                     /locus_tag="VEW08_00145"
     rRNA            15592..>15723
                     /locus_tag="VEW08_00145"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         3009 a         4837 c         5140 g         2737 t
ORIGIN      
        1 gccctcgacg cggcgcgcgc cgccgcggaa gtgatccgcg gtttctacca acgcaacgtg
       61 aagatcgagg tgaaggcgga caagaccccg gtcaccgagg ccgacgtgcg ctccgaagag
      121 gccattcgtg acctgctcac caagcgcttc ccgtcgtacg gcttttatgg cgaggagacc
      181 ggcaagtcgg acatgaatgc ggagagcgtc tggctggtcg atcccatcga cggcaccaag
      241 tcgttcgtgc gcgaatgccc gttcttttcc acgcagatcg cgctcatgcg cggcggcagg
      301 ttcgtgttag gcgtgtcgat ggcgccggcg tacgacgagc tggcgtgggc cgagcgcggc
      361 aagggcgcgt ttctcaacgg caagcggatt tccgtgagca aggtgaatac gctcgaggcg
      421 gcgatcgtct cgtccggcaa tctcaagacc ctggcggctt ctccggcgtg ggcgcgtttc
      481 ggtggcctga tcggccgcgt gaaccgcatc cgcggttatg gcgacttcgt gcactaccac
      541 ctgctggcgc gcagcgcgct cgatgtcgtg atcgaatcgg atgtgaatat tctcgacatc
      601 gcggcgctga ccgtgatcgt cgaagaagcc ggcggccgat ttaccgacct caagggctcg
      661 gcggtgaatc tcgacaccac cacggtgctt gccaccaacg gcgcactgca cgagaccatc
      721 ctggaaaacg tgcgtttcgg cagctgacca tgtcggtatt cctgcgctgg ggaattttcg
      781 gaatcctggg tgtcgcggcg ctgctgtatg cctacaacac cagcaagagt ctggccgaaa
      841 aacgctcgtc gcgtgagccg cccgtagccg ccaatgcggc aacaccggtg gaaccggagc
      901 cggtgccgac gacagctccg cattgccagg ccgaactcga ggtcgcgcaa cgcgcgctgg
      961 cggcacgtcg tgagggtgag ccgctcgacc gtctactgcg aattcaggag atcgcctggc
     1021 aggaaccggt cacccggcgc catcggctcg aggaagtggc gacgcgctgg taccagctcg
     1081 aaggcgaaga accgatcgcg gaagcattga gaatcagggt catcagcgac tgcgagcact
     1141 tcagcccggc gccatgaagc gctgttgccc cagacgcgtt tcgacgtagc ggatgccgta
     1201 gaggcgctgg aggtgatccg tgccgagtgc cgcaccgtcg accagttcac tctgtccatt
     1261 gcccgacagc agacaggcgc ggtccgcgta acgcaacgcg agattgggct cgtggaggct
     1321 ggcgatgacc gcggcgccct cacgggtgag gtggcgcagc ttgtcgagaa tgccgatctg
     1381 ctgcgctgga tccaggtgat tggtgggttc gtcgagcagg tagatagacg gcgcctggac
     1441 cagcaggcgg gcgatggagg cgcggcgttg ttcgccgcct gacagcgtgc cgagttcgcg
     1501 ctccgcgaag gcttcgaggc cgacatcggc gagcgcccgg gccacgcgtt gtgtatcggc
     1561 ggcgcccgcg gcttcccaga agccgagatg cgcgaaccgg ccaagcagtg cgctttcccg
     1621 caacgaaccc tggggagctg cctcgggatc ctgtggcagg aaccccagcc gcatcgcgac
     1681 ctcacgtcga gggacggtgg tgatgtcgcg gtcgtcgatc cgcacgaggc cggcggtggg
     1741 cgcgcgcagg cccgccagcg tccgcagcag cagggatttg ccggtgccat tgccgcccag
     1801 aagcgcgatg aattcgccgg cgcggatcgt gcaatcgaac gagtctatga gagtccggcc
     1861 agggaccgcg atgcgcaact gcgcggcttc gagcacggta ctcatttccc gatccagcga
     1921 atctcgagcg cggtggcttc gcccacgacg accttgtcga gcgactccgg aatctcgaag
     1981 gtccttgcgt cgaccgcgga acggccggcc atgatttccg cgaacggttg cggaaaacga
     2041 ggcgtcctgt ccggctccgc atagccgatg ggaaacagcg gcgccagcgt atccggatca
     2101 tatttgtccg atgcgccgag cgtatgcagg atctcgtgcg cgatgaccac gttgttgccg
     2161 cgcgtcatcc ccgcggccgc gaaggcatgt accacaccca cgaggccttt ctgcaggccc
     2221 actgaatgcg gcactgcttg ggaaaaagcg gggtcgtggt agagcacgaa gaggcgtatc
     2281 tgcggaggcg gcttgccgga ctcgcgcgcc gcgcgccgcg catacatgcg cagcttcagg
     2341 ctccaccaca tgttctgcag gacgttcgaa ctggcgccgc gttccggcgg ccgttctgcc
     2401 acgggcgggt acagatcgac agtgaccggg cgcgcgatgc cgatgccgaa ttcgcgcgct
     2461 tcctcattca ggaacttttc gatgtcggcg atgtgatcgg acgacaggcg ctcgatgtaa
     2521 tcgtgcacga ccgggttccc gggctcttcg accgggaata cgccgacgta cagggtctca
     2581 tcccagtcgg tggtgctgag gcggtcgtac cagttggacc aggcagctac gccaaggatc
     2641 agcagcagaa acgcgatgcg gacattgcgc cacacgtcag ggctgcagcc gcgtggccga
     2701 ccacgggccg gtgcgaaagg cgtcggccgt ggtagttagc gagcgggtcc agcgcgctcg
     2761 ctcgtggatg cgcgaagcgc cttcgaccca caactcgacc gagtcggccc acacgtagta
     2821 gccgccccag aagggcggtc gcggaatgtc cgcggctacg ggtacgttga tgttgtcgag
     2881 cgtgggctcg ggcacgccga accgtttcgc ggcagcgagt actgccgcgg tgagctgctc
     2941 tctcccgcgc agcggctggc tctgcgcgct ggcccaggcg ccgatgcgcc gctgccagtt
     3001 gcgcgacgcg aaatacgcgt cgctctcgga ggctggcgct ttcaccacga ttccctcgac
     3061 gcgtatctgg cgatgcgagt gatcccagtg gaacaccagt gcggcgcgcg aattttccgc
     3121 gagatcccgc cctttgcggg agtcgtagtt ggtcacgaaa gacacgcggc cctgctcgac
     3181 atcgaactcc ttgcacagca cgatgcgcgc ggaaggcagg ccggtcgctg tcgcggtcgc
     3241 gagcaccatg gcgtcgggat tgggctgttg gcgggcggcg atggcagtgg cgagccaggc
     3301 gctcgccaga gccatgggtt cggccggcag gggatcgggc agcaggtcat tgaagagttc
     3361 catggccgat atgttatccg tgatgccatt agtgatgcag tagccttgca gtcgtgcctc
     3421 gcagccgata aaagctaggt cggaattcgt ttaaaaggca aattcatgag ttcgctcgaa
     3481 gacctgcgcc gcaggatcaa cgacctcgat cgtcagatcc tcgagctggt cggcaaacgc
     3541 caggagacca gccgcgaagt ggcgcgcgcc aagcgcgcca ccggacatgc gacgcgcgac
     3601 tacaaccgcg agcgcgaggt gctgttggcg gcgcgcgagg cgggcaagca gtacggcgtg
     3661 tcgccccagg tggcggagaa catcctgcgc ctgctgatcc gttcttccct aactacccag
     3721 gagcaggcga gtgtcgccgc ccagggcgcg ggaaacggcc gcacggcact ggtcatcggc
     3781 ggcgcgggca agatgggccg ctggttcgcc gagttcatgg catcccaggg cttcgccgtg
     3841 gaaatcgccg atcccgccgg cgcgccgccg ggtttcgcac accttccgga ctggcgcact
     3901 tccccgcttg gccaggattt catcgtcatc gccacgccgc tcggagtcac cgacaagatc
     3961 ctgcgtgagg tggccatgcg ccgccccaag ggtgtgattt tcgacgtcgg ctctctcaag
     4021 tcaccgctgc gtgccgggct caatgcgctc aagtcgcacg gctgccgcgt gacttcgctg
     4081 cacccgatgt tcggcccgaa tacggaactg ctctcggggc ggcacgtgat cttcgtcgag
     4141 gcgggttcgt ccgaagcgac gtcggcggcg cgtgaactgt tcgcctccac catggtggag
     4201 caggtggtga tgtcgctcga cgaacacgat cggctcatcg cctacgttct cgggctgtcg
     4261 cacgcattga acatcgcctt cttcacggcg ctggccgaaa gcggcgaagc tgcgccacgc
     4321 ctcgcaaagc tgtcttcgac cacgttcgat tcgcagctcg acgtggcttc gctcgtcgcg
     4381 caggaaagtc cggagttgta cttcgagatc cagagtctca acgactacgg atcggaatcg
     4441 ctcgaggcgc tgtcgcaggc ggtcgagcgg ctgcgcaccg cggtgatctc ccaggatttc
     4501 gaagcgttcg ccaatctcat gcgtcgcggc cgtgaatacc tggaagaccg gcggtcagtc
     4561 gccgaacggc gtgcgtgaga gtgagctcgc gcgaccctga ctctgccccg gccgatgagt
     4621 tgcgcgagca tctgcgggtg ctcacggagg aggtcgcgcg caacgagctg ttgctgcgca
     4681 agacccaggc ccgcgaaatc gagctgcttc gcgcgaattc actcggcgag ctattcgacc
     4741 ggatcgttac cgggctgcgt gacgcctatg cactcgacct cgtcaccctc tcgttgttcg
     4801 atccccagca cgagctgcgg catctatccg gtaacgaaaa cctgacgacc gcggtcatcg
     4861 aggaagtacg gttcatcgat gccgtgtcgc cacttgcgcc gcagctcgcg caattcgacc
     4921 ggccctggct cggccgttac tccgccgtcg atcacgaatt gctcgtgtcc ccggcgatga
     4981 aacagggcag cgtcgcactg atcccgctac cccgcgacag ccgcgccatc ggcgtgatcg
     5041 cattctgcag ccgcgatcca caccgcttca ccagcgacct ggccgcggac tttctcgcgc
     5101 acctcggcgt catcgtcgcc atcagcctgg aaaacgcggt aaatcgcgcg cgcctggtgc
     5161 gcagcggtgt caccgatttc ctcaccggat ggcacaaccg ccgctatttc cacaatcgcc
     5221 tacgggaaga actcgcgaga tcggagcgca ccggcaagac gctggcctgt ctcatgatcg
     5281 acctcgacaa cttcaaggac atcaacgatc gcttcggcca cctggccggc gacgaggcgc
     5341 tcaaggaagt ggcccggcgc gccgaggccg agattcgcgc gggcgacacc ggcgcgcgct
     5401 tcggtggcga cgagttcgcg atcctcctgg ccggtgcgga aatcgcgcac gccctgaagc
     5461 tcgccgagcg catccaccat agcgtcggtt cgacgccggt tcgcgtgcat ggcggcgcgc
     5521 atgtgaccgt gaccctgtcc gtcgggctcg ccaccgcgcg gccggagtcg aggtcgcacg
     5581 attacaaggc gctcgccgag cggctcatag ccgaggctga tgccgcgctg taccgcgcca
     5641 agagcgctgg acgaaacagg gtcgagatgt cgacccatgt catcggctga cggtcaggtg
     5701 gaccgcgggc cttcgagccg tcgcagcaga accaggaaca tcggcacacc cagcagcgcc
     5761 atgatggcgc ctaccggcaa ttgccgtggg gccgccgcgg tgcgtgccac gagatcggct
     5821 gcggctaaca aggttcctcc ggcgagcgcc gcagctggcg ccacgacgcg atgcgcggcc
     5881 gtcccgaacg cgagtcgcac caggtgcgga atcacgaggc cgatgaatcc gatggtgcca
     5941 ccctggatca ccgcgcccgc ggtcagcagc ccggcgaata ccacgatgac cattcgccag
     6001 cgtggcaggt ccagccccag gctctgcgca gtgatttccc cggtcgaaag cacatccagc
     6061 ggtcgcgcga tcagcgtcac ggccagcgtg gctaacaagg ctgcggcgag catcacccac
     6121 gggtgcgccg cccagccgag atctccggcg agccagaaca tcatgccccg cagctggccg
     6181 ttgtcggcca gcaccaggac cagcgtcacc agcgcgccgc aggcgctcgc gaccacgaca
     6241 ccggtgagca ggacgcgcac cgtggcgccg ccgcgcgcca gcgcgagcac gaggaatgtg
     6301 gctgcgatcg ctccgaaggc cgccgaacac tggaccgccc agagggatgc gccgagcaac
     6361 agcgcaaaca aggcgcctac ggacgaaccg cccgagacgc cgagaacata tgaatccgcg
     6421 agcggattgc gaaacaatgc ctgcagcaac actccggcga gcgccaggag cgcgccgatg
     6481 gtgaatgcgg agagcagtcg cggcgcgcgt aattccatca cgacggttcg ggccggttcg
     6541 gcgggatcac cgaacaaggc agcgatagcc gggcgcacgc cgatgtcggt ggaacccgag
     6601 accagcccca ggcaggtgac gatcgccgca agtgcgacga gcaaaacaag taacgcagtg
     6661 gtgggtgggc gcgaagtcac gcggcattgt ccgataaaac ccgcgcagcg tgtctctttg
     6721 ccttgttcgg ccgtgcgcca cccggtaata cttgcatcat gagcgactgg atcgattcgc
     6781 agggatatcg agccaatgtc ggcatcgtcc tgatgcgcga cagcggcgag gtcttccttg
     6841 ggcagcgcac gggtgcacgc ggctggcagt ttccgcaagg gggcgtgcgg cagggcgagc
     6901 ccgtggagca cgcgctgttt cgtgagctcg aggaagaaat cggtctccag ccgcagcatg
     6961 tgacgctcat gggctctact gccgactgga tacgctaccg gctgccgaag cgttatgtgc
     7021 gtcgcgggcg cggtcccacc tgcataggcc agaagcaacg ctggttcctg ctgcggctcg
     7081 cggtacccga agatgcggcg gagttcagtt tcacgaaaac cagcgaaccg gagttcgacg
     7141 ggtggcgttg ggcaaactac tgggagccgg tgcgtgaagt gatctacttc aagcggccgg
     7201 tgtacgtgcg catgctcaca gaacttgcgc cgaccgcatt tccagccggg ccaccgcccc
     7261 tgccggaatg gtggcaatcg gaaaccgccg caacagtgga agaatagcgc cccgatgccc
     7321 ggtcttcctc ccaaactcgt cgtcacgacc tacgcgccga cgcgccggtg gatcaccatc
     7381 gtggtgctcg tattcatcgt cgccgccggc gtctatggaa tgtacgagtt cggccgctac
     7441 cgtgccgggt acgacgtcgt cgcgggcctc aagcagcgca cggaaatgcg caacgaaata
     7501 gccacgctgg aggccacgat cttcgatttg cgcgccaaag tggcgcagct ggaatcctcg
     7561 accgttggac agacccggga gcgggaagag gtgcagcgca cgatcggcga tctgcaggcc
     7621 caggtggccc gggcgaacca ggaattggcc tttttcaggg ggatcgtcac ccaaaatgcg
     7681 aactctgccg aggtaaaaat tcagcaggcg cgtatggtgg caacggccac ggcaaacaag
     7741 ttccgcgttc gcgtcaccct ggtccagccc atgaagccgg atactgtggt gactggcgtc
     7801 gttgtcttgt cggtcgacgg tgaagtcgat ggcaagccgg ggcgtgcgga tttcgcgaca
     7861 ctcaccggcg gcaagcgtcg tgagatagcc ttcacctttc gatatctcga gaacattgac
     7921 gaggaaatca ccatgcctcc gggcatgaaa cccgagcagc ttctggtcga agttcgatcc
     7981 aatcgccgcg ggtccgcgcc ggtacagcag tcgtatgtgt ggagcgtcga tccgcagtga
     8041 gcggagcgct caggagaggg taatgttcgg tcgtcgcaag cagtcatcca cgcgcatcga
     8101 tacgcttctc gggaaaacag ccacgctcaa tggtgacctg gaattctccg gtggcctgca
     8161 tctagacggc cgtgtgaacg gcaatgtatc cagcaacgcc accgatggag gcgccctctc
     8221 ggtcagcgag agcggcttca tcgaaggtaa tgtcgaagtc accaatatcg tcatgaacgg
     8281 caccgtgaac ggcgacatgc atgcgcgcga tcgcctggtc ctcggaggct cggcgcgcgt
     8341 caatggcaat gttcactacg gtgtcattga aatggcgccg ggcgccgtca taacgggaaa
     8401 actgataccg ctgggcaaag acaaggcccc ggaaacgcag ccggaagcag cgcctccggt
     8461 tgcggcagcc aaatccggcg cgtagtgtgc aggccatgag cgaaaccgag accgaaaccc
     8521 tcaaattcac cgatgccgcc gcccgcaagg ttggcgacct catccgtgga gagggcaatc
     8581 ccaatttgaa gctgcgcgtc ttcgtgcagg gcggcggttg ctcgggcatg caatacggct
     8641 tcgaattcga cgaacaagag caggatggcg acacctgcgt cgaaaacctc ggcgtgaagc
     8701 tgctcatcga tccgatgagc gtgcaatacc tcacgggcgc ggaaatcgac taccgcgagg
     8761 gcctcgacgg ggcccagttc gtcattcgca atccgaacgc ccaaaccact tgcggttgcg
     8821 gctcgtcgtt ctccgcctga cgccagctcg ctagacgtgg cgcggcgcgc tccgaagtcc
     8881 gtccccgacg tacttgctgc ggtcgatctc ggttccaaca gttttcacat ggtggtcgcg
     8941 cgttacacgc acggccagct ggtcattgtc gatcggctcc gcgagatggt gcgcctcggt
     9001 gccggcctcg aggccgatgg gcgactgaac aaggacgttg cggcgcgcgc gctcgcctgc
     9061 ctcgaacgat tcggccagcg cctgcgggac atgggacccg gcagtgttcg tgttgtgggc
     9121 acaaacgttt tccggcgcac gagaaagaag gaagcgttca tcgagcgtgc gcgcgaagca
     9181 attgggcatc ccatcgaaat cgtttccggc atcgaagagg cgcgcctcat ctactccggc
     9241 gtggcgcaca cgatggcggc gggtgccggt cgccgcctgg tctgcgacat tggcggcggc
     9301 agcacggaga tcatcatcgg tgagggtctg gagcccctgg agctcgagag cctgcagata
     9361 ggctgcgtgc gcctgagcga agaatttttt gccgagggca gactctctca gaagcgcatg
     9421 cagcgcgcgc gggtcgcggc gcggcaggcc attgagccgt accaggccgc gttccgccgc
     9481 cggggattcg acgaggccgt gggaagttca ggcaccgtgc gcgccatcgg cgacgccatc
     9541 cgcgagctcg atcccgccgc agtcgacatt ggcgccgacg gcctcgaacg catcatcaat
     9601 gaactcggcg ggctcgagca cattcgcgac ctgaagctca gtgcaatcac ggaagatcgc
     9661 aagcattcgt tcgccggggg cgtggcgatc atggccgaag tgttcgagca gctcggcatc
     9721 gaaaccatgc gcgtggcaga cggcgcgatg cgcgaagggc tgctctacga cctactgggc
     9781 cggtacaccg acgaagatgc gcgcgagcgt acggtacgca gcatgcagca gcgctaccac
     9841 gtcgatctcg tccaggcgga acgcgtcgaa gcgacggcgg tcgaattcct ttcgcaggtg
     9901 gaggattcct ggtccctcgc ggatccggcg atcgagcagc tgttgcgctg ggcagcgcgg
     9961 ttgcacgaga tcggcctcga cgtggcgcac agcggttacc accgccacgg cgcctatcta
    10021 ctggcgaatg ccgacatggc gggattcccg cgcgaggagc agaagctgct ggccatcatg
    10081 gtgggcagcc accggcgcaa acccgcgatc gaggaaaagg atgatctcgc agcgccgtgg
    10141 gacaagcgcg ctccggcgat gacgctgttg ctgcgcctgg cggtgctcct gcatcgcggc
    10201 cgcagcagcg tggcgctgcc ggccatcaag ctgggtgcgc gcgccgattc gctggaaatg
    10261 cgctttccgc cggattggct cgacgatcac ccgctgaccg tcgcggatct gcagcaggaa
    10321 gtcgagaacc tgaagagcgt cgccttcaga ttgcgcgcgt ttaccgccaa gtagcgctgg
    10381 ggtcagggta ggacggcggg ggcgctgccg gccgcgtagc gagccaggag cgctccctgt
    10441 gcatcgcgcg ccggatcatc gccgcgaacg caacgcgagt agtgaccatc acgcccgagc
    10501 acccaggcgc gcgtattgtc ggagaggtag gaattcaggt cgcgcacgat gcggtcccga
    10561 tgcgtctctt cgcggaccgg gaaggcgatc tccactcggc ggaagaagtt gcgttccatc
    10621 cagtccgcgc tcgcaagata cagctcgctg gcgccatcgt tgccgaagaa gaacacgcgc
    10681 gagtgctcga gaaagcggcc gacgatcgac cgaacccgga tattctcgga gactgcggga
    10741 attcccggtc gcaacgagca caggccgcgg atgatgaggt cgatctgcac gccggcacgg
    10801 gaagcacggt acagcgcctc gatgacctgc gggtccacca gcgagttcat cttggcgaga
    10861 atcctcgcgg gttttccagc cgcggcatgt tgtgcctcgc gctcgatctt ctccacgact
    10921 ttctcgtgca atccgaacgg cgactgcagt agtttggtga gccgcggtgt ctgcgtcagg
    10981 ctggtgagct gcaggaacat ctcgtgcaca tcctggccaa tcgtcgggtc gcaggtgaac
    11041 aggctgtagt ccgtataacc gcgtgccgtg cgcgggtgat agttgccggt gccaaggtgg
    11101 cagtaccggc gcagtccgtc ggattcgcgg cgcaccacca tcgacatctt ggcgtgagtc
    11161 ttgaagccga ccacgccata catcacgtgg gcgccggctt cctgcaggcg gctggacaac
    11221 tcgatattcg cttcctcgtc gaacctcgcg cgcagctcga cgatgaccgt cacatccttg
    11281 ccggcctgcg cggcagagac cagcgcatcg acgatcggtg attcattgcc ggtgcggtac
    11341 agcgtctgct tgatggcgag cacgcgcggg tccgtggctg cgcctcgcag gaaatcgagc
    11401 accggaccga aactgtggaa tgggtggtgc agcaagacat ccttctggcg aatgaccgcc
    11461 agcaggtcgg tggagcttgc gacccgcctc ggcaaccccg gtgtgaagat cgggtatttc
    11521 agatccggcc gctgggcgag atcgtagatc gcatcgagcc ggttgaggtt gaccggtccg
    11581 gaaacctcgt agaagtccac ctcgttcaag gcgaactgcc gcatcaggaa ggcgttgaga
    11641 tccggagggc agtccttcga tacctcgagc cgcacagcgg ctccgtaccg tcgatgtgca
    11701 agttcgccct cgagcgcccg gcgcaggtca tccacctctt catcatcgac gaagaggtcg
    11761 ctgttgcggg tcacccgaaa ttgatgacag ctgagcactt ccatcccgga aaagagccgg
    11821 gaaacgaatt gctggacgat tcctgtgaga agcacgaatc gtttgagtcc gttggcctcg
    11881 gggagcgcga ccacgcgcgg cagcgagcgc ggcgcctgga ctatggcgag accggagtcg
    11941 cgaccgaagg cgtcctgacc ggcgacgcgc acgatgaagt tcaggctctt gttctggatt
    12001 cggggaaacg gccgtgccgg gtcgagggcg agaggcgaca gcaccggctc gacctcgagt
    12061 tcgaaatatc gctcgagcca ttgggccgtc gccgagtccc aggtcgttcg cgccagtagt
    12121 tcgatgccct gacggcgcat ccccggcagg accacttcgt tcaggcaggc atattgagcg
    12181 tccaccaggc ggcgggtgcg ttcgtgaatg gcctgcagct gttcggttac ggtaagtcca
    12241 tcggcgccgg gctgatccga gccaagctcg agccgctgct tgaggccggc gacgcgaatc
    12301 tcgaagaact cgtcgagatt gttcgacgag atgcacagga atctgagccg ttcgagtaac
    12361 ggcactttct catcgagcgc ttgcgccaga acccgaccgt tgaactcgag cagcgacagt
    12421 tcgcgattga tgtagtgctc gggggaatgg agatttggtt gatccatggc gcgaagactg
    12481 ggtttcgcgc tatgaaatca cagctccgga ccgagtaatg tgaaagttgg cgtcagcgcg
    12541 ctacgagcgc ggggccggcc ggcaaatcca gcatgttcac gaggagcgcc gtgtcgttca
    12601 ggaagcgctc ccgttccgcg tttggaatgg gttcagcatc cggcagctgt acagtttgtg
    12661 gattcttgtg aacgccgcgg atccgatact catagtgcag atgtggcccg gtggcgaggc
    12721 cggtcttgcc gacatagccg atgacctggg cgaggtcgac gtgctggccg cgccgcaact
    12781 ttgtggcgaa gcgcgaaagg tgaccataaa ccgtcaccac gccggaccca tgttctagct
    12841 cgatgacatt gccgtagccg ccttgctgcc ccacgaaacg cacgcgacct tcgccggcag
    12901 cgcgcaccgg cgtgcccacc ggcgcggcgt agtcgacacc tcgatgtgcg cggatacgat
    12961 tcaacaccgg atgccgccgc gaggggttga atcgcgaact gatgcgcgag aactgcacgg
    13021 gcgcccggat gaaagccttg cgcagactct tgccatccgg cgagaaatat tgaccgcgcc
    13081 catctacatc gacgaaacgc acggcgcgat attcgcggcc gtcatttacg aacttcgcgg
    13141 cgagaatatt gccgattcgc agcggaacgc cgtcctggaa aacttcctcg tatacgacgg
    13201 tgaaacgatc gccggactgg atgtcgagca cgaagtcgat gtcgtactgg aaaatctcgg
    13261 cgagttcgaa agccacacga tcggagagtt ctgcatccgc agccgcctga aacagggagt
    13321 tctgaatcgt cgcgctggcg gttcgtgtgc gtacttcgag cggattctcg aggacgtcac
    13381 tgctgaagcc gttggcgtcg cgggtcactt tcagtgtctc gctgtccgac agtttgcgtt
    13441 cgaggccgac gagttcgccg ccgcgatgca tgaagcgcaa caattcgccc ggcttcagtc
    13501 gatcgacctg gctgcgaagc tcgggcagat tgcgcaagtt cgcgaggtcc ccgagattca
    13561 attcgaagcg gcgaaagagc tggtccatcg tgtcgttgcg gctgacgacc acctcgatgg
    13621 tggagaaacc gaggctggcc tcatgcgtgc ccggagggga ttggctcgcg ggcgtggccg
    13681 gcgcctcggc tgagggctgg gcggcgagtg ggggcatctc gcgatcgcgc ggcaatgcca
    13741 cgagcgcagc gacaatggcc accagcccga tctggagcag gtatgcggtc cgggtcggca
    13801 atcccggaat ggtggacttg atgaagatcg gcacgatcta tacaacgtaa cccacggatc
    13861 gataaagggt caatcgatgc gctttcagaa tgtgatcgcg ctttggaaac ctcccgatct
    13921 cctaataaca acccgatgca tcgaagagac gaatggacgg cgaattgcag aatcagatgg
    13981 cggaactgct gcgcggaacc caggaagtgc tggttgaaag cgagcttgta aaaaagttaa
    14041 tccgcggcaa gccgctgcgg atcaaggcgg gattcgatcc aacggccccc gacctgcacc
    14101 tcggacacac cgtgctcatc aacaagatgc gccagttcca gaatttcggt cacgaggtca
    14161 ccttcctcat cggtgacttc accggaatga tcggcgatcc gaccggccgt aacgcgaccc
    14221 gtccgccttt gacgcgcgaa caggtcgacg caaatgcgca gacctatcag gagcagattt
    14281 tcaagattct cgatcgcgac aagacgcgcg tggacttcaa ctcgcgatgg ttcggcggga
    14341 tgacggcggc agggctcatc gaaatcgcgg gccgacatac ggttgcgcgg atgctcgaac
    14401 gagacgattt cgcgaagcgc tacaagggca atcagccgat cgccatccac gaatttctgt
    14461 acccgctcgt gcagggctac gattccgtgg ctttgaagag cgacgtcgaa ctcggaggca
    14521 cggatcagaa gttcaacctg ctggtcggac gacagttgca ggagagctac ggccaggagc
    14581 cgcagatcgt catgacgatg ccgctgctcg aggggctcga cggcgtcaac aagatgtcga
    14641 aatcgctggg taattacatt ggaatcaacg aatcggccga catccagttc ggcaagatca
    14701 tgtcgatttc cgatgacctg atgtggcgct acttcgaact gctttctttc cggccgctcg
    14761 cggatgtcgc ggcgctgcat aaggcagtct tggaaggtcg caatccgcgc gacgtgaagt
    14821 tcgagctcgg ggtggagatc gtcgatcgct ttcacggcgc tggtgccggt gtgcggtccc
    14881 gcgaggcgtt tgtcgcgcgc tttcaacaag gccaggtacc cgagcagatc gagagcattt
    14941 ccctaactac tgacggatcc ccgcagcgtc tcgcgaacgt gctgaaaagc gccggtttgg
    15001 tggcgagcac ctcagacggc aaccggatga tcgaccaggg cgccgtgcga atcggcgata
    15061 gtgccgaaac cctgtcaaaa gtggccgggc gcgaccacgt gcttggcccc ggcaagtttc
    15121 tggtccaggt ggggaagcac aaattccgct gggtaacgct gcaatccgga taggggcggg
    15181 cgggagacgg tcacgaccgc atatttgtaa ttcccggccg agtttgagca ggttttcgcc
    15241 aacaaaaaca cgcatacatt caagggacta ggcgaaatct gacgctattt gtgactgtgc
    15301 gttgacagac cggattccct ccctataatg cgccccctca cgcaccgacg gccttcgggc
    15361 ccaagcgcgg tgagtgagat aaatgttcga aacaaagttg acggaattgg gctccggcct
    15421 ataattccgc ccccttctgc ggccctcagc ggccgtagtg ctgtctaaaa atttgcaggt
    15481 aatttgtgtg gggactcgcg tcgatgactc ctttggagac attaaccgag taaccaaaaa
    15541 gtccagcaat gggcctttgg atactctttg atttgaagtt ctgaagatct ttaagtgaag
    15601 agtttgatcc tggctcagat tgaacgctgg cggcgtgcct aacacatgca agtcgagcgg
    15661 taacaggcgt agcaatacgt gctgacgagc ggcgaacggg tgagcaatgc ttcggaatct
    15721 gcc
//
LOCUS       DASQJS010000004         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_944886, whole genome shotgun sequence.
ACCESSION   DASQJS010000004 DASQJS010000000
VERSION     DASQJS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944886"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1483)
                     /locus_tag="VEW08_00150"
     CDS             complement(<1..1483)
                     /locus_tag="VEW08_00150"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HYJ39165.1"
                     /translation="MRPLSKIVIVGGGTSGWLAASMLCQHLKRELCEIELVESEELGT
                     IGVGESTVPPFVGLIQRLGIDESEFVRATDATYKLGIKFVGWHQRSDSYFHPFGVIGK
                     PIGSHDFYQCWLKARAEGNTDALQDFSPCNVMAEQGRFFHPAKARNTPIGGANYALHV
                     DALLVTKFLRKYAEERGLKRTEGKVVEVGQRNDGFIESVTLASGQKIEADFFIDCTGF
                     KSLLIGKTLGVENIDWSNYLPCDRAVVCKTENKGPLVPYTRATAQPAGWSWRIPLKQR
                     VGNGYVYSSRFVSDAAAKATLLRSLDAACIEEPRLIPYTTGHRRQFWKHNCLSLGLSS
                     GFIEPLEATSIHLIARGMDFFLRYFPDKDCDAALMREYNRRMVADFEEVRDFIVLHYS
                     ATARDDTPFWQWCRNIPLPDTLRERIELFKAHGSMREGVDELFRASSWQSGFEGMGIR
                     PNGHCPRVENLDYAQIEQTLRNAKAAIAGMVEHLPTHEQFLRTA"
     gene            complement(1483..2454)
                     /locus_tag="VEW08_00155"
     CDS             complement(1483..2454)
                     /locus_tag="VEW08_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007894647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HYJ39166.1"
                     /translation="MTLTELRYIVALAQEQHFGRAAERCHVSQPTLSIAVKKLEEELG
                     VLFERGKQKILPTPLGEKIVAMASAMLEQAAAIKDVADADRDQLEGPIALGTLPTIGP
                     YLLPQFIPLLQETSNKLSLYVEEGSVSELASKLRSGDLDAVLVTAPFIEADVVTQPLF
                     DEPFVVLLPADHRLAAKVEISGSDLLPTELLLLGEGDSMREQILAAFPHLDPHLETGA
                     GVRTFTQGATLETLRHMVASRLGLTILPQTAAEAPLYAPNLLVTRPFAKPVPRRSLVL
                     AWRVSFPRHKAIDLLRRAIQASSSAYWNYNTARAQEAPGVMVENRNW"
BASE COUNT          468 a          828 c          824 g          483 t
ORIGIN      
        1 aggcagtgcg caggaactgc tcgtgcgtgg gcaggtgttc caccatgccc gcgatggcgg
       61 ccttggcgtt gcgcagcgtc tgttcgatct gcgcatagtc caggttctcg acccgcgggc
      121 agtggccatt tgggcggatg cccatgcctt cgaaccccga ttgccagctc gaggcgcgga
      181 acaactcgtc gacgccttca cgcatcgagc cgtgcgcctt gaacaactcg attcgctcac
      241 gcagcgtgtc gggcaatgga atgtttctgc accactgcca gaacggtgtg tcgtcgcgcg
      301 cggtggcgga gtaatgcagc acgatgaaat cccgcacttc ctcgaagtcc gcgaccatgc
      361 gccggttgta ctcgcgcatc agcgccgcgt cgcaatcctt gtccgggaaa tagcgcagga
      421 aaaaatccat gcctcgcgcg atcaggtgga tggatgtcgc ctcgagcggc tcgatgaacc
      481 cggacgacaa acccagcgac aggcaattgt gcttccagaa ctggcggcga tgccccgtgg
      541 tgtacggaat gagccgcggc tcttcgatgc aggcggcgtc cagcgaacgc agcagcgtgg
      601 ctttcgccgc ggcatcgctc acgaaacggc tcgagtacac atagccgttg ccgactcgct
      661 gcttgagcgg aatccgccag ctccagccgg ccggttgcgc ggtggcgcgc gtgtacggca
      721 cgagcgggcc cttgttctcg gtcttgcaga ccacggcgcg atcgcagggc agatagtttg
      781 accagtcgat gttctcgacg ccgagcgtct tgccgatgag caggctcttg aagccggtgc
      841 agtcgatgaa aaagtcggct tcgatcttct ggccgctggc gagcgtcacg ctttcgatga
      901 aaccatcgtt gcgctggcca acttcgacca ctttgccttc ggtgcgcttg agcccgcgct
      961 cctcggcgta cttgcgcagg aacttggtga ccagcagcgc gtcgacgtgc agcgcgtagt
     1021 tagctccacc gatcggcgtg ttgcgcgcct tcgccggatg gaaaaatcgt ccctgctccg
     1081 ccatgacgtt gcagggagaa aaatcctgca gcgcgtcggt gttgccttcg gcgcgcgcct
     1141 tgagccagca ctggtagaaa tcgtgactgc cgatcggctt gccgatgacg ccgaacggat
     1201 ggaagtagga gtcgctgcgc tggtgccagc cgacgaactt gatgcccagc ttgtacgtgg
     1261 catcggtggc gcgcacgaac tcggattcat cgatgccgag gcgctgaatc aatcccacga
     1321 acggcggtac cgtcgactca ccaacaccaa tcgtgccgag ctcttccgac tccaccagct
     1381 cgatttcgca gagctcgcgc ttgaggtgct ggcaaagcat ggaggccgcg agccacccgg
     1441 aggtgccacc gccgacgatc acgattttcg agaggggccg catcaccagt tccggttttc
     1501 caccatcacc ccaggggcct cctgggcacg cgccgtattg taattccagt aggccgaact
     1561 gctcgcctga atcgcgcggc gcagcagatc gatggcctta tgtcgcggaa aactgacgcg
     1621 ccaggccagc accaggctgc gtctgggcac cggtttggcg aatggccggg tcaccagcag
     1681 attcggggca taaagcgggg cttccgccgc cgtctgcgga aggatggtga gccctagccg
     1741 cgaggccacc atgtgtcgca gggtctccag agtggcgccc tgcgtgaagg tgcgcacgcc
     1801 ggcgccggtt tccagatgcg ggtccaggtg cggaaacgcc gccagtatct gttcacgcat
     1861 gctgtcgccc tcgcctaaca acagcagctc ggtgggcagc aggtccgatc ctgagatctc
     1921 gaccttggcc gccagccgat gatccgccgg cagcagcacc acgaagggtt cgtcgaacag
     1981 cggctgcgtg accacgtccg cctcgatgaa cggcgccgtc accaggacgg catcgagatc
     2041 gccactgcgc agcttgctgg cgagttcgct gacgctgccc tcttccacat acaacgacag
     2101 cttgttagac gtctcctgca gcagcgggat gaactgcggc aacaggtatg ggccgatggt
     2161 gggcagcgtg ccgagggcga tcggaccttc gagctggtca cgatccgcgt cggcaacatc
     2221 cttgatggct gcagcctgtt cgagcatggc ggaagccatg gccacgattt tctcgccgag
     2281 cggggttggc aggatcttct gcttgccgcg ctcgaacagc acgccgagtt cctcttcgag
     2341 cttcttgacg gcgatggaca gcgtcggctg gctcacgtgg catcgctcgg ccgcgcggcc
     2401 gaaatgctgc tcctgcgcga gggcgacgat gtagcggagc tcggtgaggg tcatgggcaa
     2461 ggatcattac tcgatccgcc tcctgcgtcc aacaccctga tcccacggcc aactgtatac
     2521 agaagcgcca cgagcctttg agcgcgccaa tcggcggagt ccgccgcctc ctttacccaa
     2581 gacgcgcctt gcgcatcatc gga
//
LOCUS       DASQJS010000005        17763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3143979, whole genome shotgun sequence.
ACCESSION   DASQJS010000005 DASQJS010000000
VERSION     DASQJS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17763
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3143979"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3299)
                     /gene="dnaE"
                     /locus_tag="VEW08_00160"
     CDS             complement(<1..3299)
                     /gene="dnaE"
                     /locus_tag="VEW08_00160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HYJ39167.1"
                     /translation="MKSAAAFVHLRLHTEFSLQDSVVRIPELVERVVALGMPAVAVTD
                     QNNLFAMVKFYREGLKCGVKPIIGVDVQMRAGERLPPNRLTLLCKDPGGYQNVTNLVT
                     RAWLEGQDRGVPLIDRSWLTPETTTGLIALSGFAEGDVGRAIANGREADALVLAREWA
                     ELFGDRYYLELQRLGRADDESMVARTVSLSQKAGIPVVATNDVRFLAASDFESHEARV
                     CISDGAQLADPGRQRRYTEAQYLRSPDEMAKLFADIPEALANSVEIARRCSLPLKLGE
                     SRLPNYPLPEGTTVDAHIRAESERGFAEREKVFDASQLARIQVYRDRLQRELGVICQM
                     GFPGYFLIVADFIRWARANGVPVGPGRGSGAGSLVAYSLGITDIDPLKYDLLFERFLN
                     PERVSMPDFDIDFCMDGRDRVIEYVSQKYGRERVSQIITYGTMAAKAVVRDVGRVLGM
                     GYGYVDKIAKLIPFELGITLADALEKEPELKRLYKEDEEVKNLIDLAMSLEGLTRNAG
                     MHAGGVVIAPSKLTEFAPLYADDKGGSVVTQFDKDDVEAAGLVKFDFLGLRTLTVIDR
                     AVKIINARRAAAGEALLDISQLPMDDQATYQLMSEAKTTAMFQLESRGMKDLIRRLKP
                     DRFEDIVALVALFRPGPLESGMVGDFIDRKHSRHDPHAPPIDFLHPSLEPVLAPTYGV
                     ILYQEQVMQIAQVLASYTLGGADLLRRAMGKKKPEEMAKERGKFVSGAVKNGVPEGQA
                     AHIFDLMEKFAGYGFNKSHSAAYALLSYQTGWLKTHEAASYMAAVLSADMDHTDKVVG
                     MLYDCRKEVGLTVLPPHVNLSAYEFAVVDDKTIRYGLGAIKGVGQGAVESIVNERRAH
                     GDFTSLEDLCRRLDLSKINRRVLEALIKSGSLDGLAANRATLMHRLDAALALGEQNSK
                     ANETGQHDIFGLSAAHVPEAVRATAPDQPEWTDSVRLRAERETLGRAISGHPIDRFVN
                     DLPRFITGRIVDYIDAEKPQASAEGGRAFFGGKPVAIAGVVEELRKRGPRTEVVLDDD
                     SGRISVGFFDEQYQQYREVLMKDALLLIEGKLRFDEFANTWVLRAGKVSELERLREKE
                     ARRIVLKLKST"
     gene            3642..4079
                     /locus_tag="VEW08_00165"
     CDS             3642..4079
                     /locus_tag="VEW08_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HYJ39168.1"
                     /translation="MAYRNLRLAGTLMLIACGVSALASAQTPSTLYARLGGTPVVSAF
                     VADALDKVVASPQMNRTLDKASLQLMKDLLTERICTLTGGGCTHGGDVMREVRTDPHI
                     SGAEFTGLIEAMRESMRAHDVPLAARNQLLEILIPMNQDVARL"
     gene            4113..6563
                     /locus_tag="VEW08_00170"
     CDS             4113..6563
                     /locus_tag="VEW08_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ39169.1"
                     /translation="MRIQRLQTRIIVLFVALLTLVQVAAFWFVNAANSRNAEDKVAEE
                     LNVGQRVFARLLDQNAEKLKLSARVLAADFAFREAIATHDTGTIASALANHGARIGAE
                     AMVFVDLQGQVISDTLSTGATARPFEYPALLKSDRNAASASMEVLDDRAFQLVAVPVL
                     APLPIGWVVMGFAVDDSLASDLRQLTELEVSFTLDGQPDRRMLASTLGLREQQELLRH
                     MPSRSNSVTRMVMDIRGVDHQALVIPLDDNDNARIVAVLHRSLGDALAAFERLRNTLF
                     VLAALSLAMSIVGSVAVARNITRPLETLATAAARIEQGDYSAPVDLRRADEIGVLASS
                     LNHMRGSIADRERRILKLAYEDPLTDLANRSRFSSDLELAIAQAALRDLKLTILMMDL
                     DRFKYVNDTLGHGVGDHVLREVSARLQRTVTDAECIARLGGDEFAILVRHADDDFAET
                     ARKIIAALEAPILYEGQPLDVGTSIGIAHFPEHGRDAQTLVRNADIAMYAAKRNKTGF
                     ATYEQHYDTSQQEHLSLLSELRRAVERNELRLYYQPKVSLHSSHVSAVEALIRWEHPT
                     RGLVPPCQFIPFAEHTGYIKILTRWVIREAVRQCGEWLRSGLALQVSVNISARDLMNR
                     ELPEHVAELLAEHDVTPGLLCLEITESGFMEDPTHAQKVVDRLAELGVKLSIDDYGTG
                     YSSLSYIMKLPVQELKIDQSFIARMATDEEISTIVRSTIDLGHNLGLQVVAEGVEDAA
                     AWEMLRTLGCDDAQGYFMSQPLEPQALANWIRANKGNFACDSQGLRPTSENAAEAAHS
                     QGLRPTSENAAEAASSNK"
     gene            complement(6573..7187)
                     /gene="rnhB"
                     /locus_tag="VEW08_00175"
     CDS             complement(6573..7187)
                     /gene="rnhB"
                     /locus_tag="VEW08_00175"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HYJ39170.1"
                     /translation="MKLRVRPRVAGVDEAGRGPLAGPVVAAAVILNPARPIRGLADSK
                     TLEPEERESLAILIRNRALCYAVAWADAEEIDSINILQATMLAMRRALMGLAVPPQQV
                     FIDGNRCPCADGLGFECLFEAVIKGDATVPSISAASILAKTTRDALMRDLDHRYPGFS
                     LSGHKGYPTPAHVAALNALGPSPLHRRSFAPVRLSDCIEEADFG"
     gene            complement(7184..8338)
                     /gene="lpxB"
                     /locus_tag="VEW08_00180"
     CDS             complement(7184..8338)
                     /gene="lpxB"
                     /locus_tag="VEW08_00180"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HYJ39171.1"
                     /translation="MRIALVAGETSGDTLGAALIEALRRQYPQAEFAGVAGPRMKAAG
                     CAAWHDIEELAVMGLTEVLTHLPRLMRLRAELRRRILEWRPDVFIGVDYKEFNLGLAR
                     QLKREGLATVQYVSPQVWAWRQGRVRTIGASVDLVLCLFPFEPDFYREHGVRAAFVGH
                     PLADQIPMVADRAAARAELGLDPSVRVLAVLPGSRRGEVEKLADVFAGAAELLATRFS
                     GLVSIAPMVTPVLRDLFAARCAALAPHAQVRLLDGQARRVLAAADVVLVASGTATLET
                     ALFKRPMVVAYRLGAITAFLLRTLGLVKIKHFSQPNLLAGKELVPEFFQEAASPANLA
                     NALAHWIEHPADVARVQQEFEAIHTRLRCDGADRAADEVAALLATRAMPA"
     gene            complement(8353..9129)
                     /gene="lpxA"
                     /locus_tag="VEW08_00185"
     CDS             complement(8353..9129)
                     /gene="lpxA"
                     /locus_tag="VEW08_00185"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HYJ39172.1"
                     /translation="MSGIDPRAIVSPKAELASDVTVGAYTVIGDGVTIGAGTWIGPHA
                     VVNGPTSIGAGNKIFQFASIGDAPQDLKYKGEPTRLEIGDRNVFREFTTMNRGTAQGG
                     GVTIIGNDNLFMAYSHVAHDCRVGSKCVMANYATLAGHVELGDWVIMGGYAGTHQFTK
                     VGAHAFIGNNAAVTRDVPPYVMAVGTPAVPHSINSEGLKRRGFTPEQIRNLKNAYRVL
                     YRSDLKLVDAVAELQSRAATQPELKIFVDFIGESTRSLVR"
     gene            complement(9126..9578)
                     /gene="fabZ"
                     /locus_tag="VEW08_00190"
     CDS             complement(9126..9578)
                     /gene="fabZ"
                     /locus_tag="VEW08_00190"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HYJ39173.1"
                     /translation="MGVRRLLPHRYPFLLVDRVLECVKGESIRAIKNITANEPFFSGH
                     FPHRPVMPGVLIIEALAQTAGLLAFMTADVVPDDSTRFFFVGIDKARFRKPVEPGDQL
                     ILTARLERSMRGIWKFSAVAYVGDDEVTSAELMVAPETNKTSAKAPAA"
     gene            complement(9609..10652)
                     /gene="lpxD"
                     /locus_tag="VEW08_00195"
     CDS             complement(9609..10652)
                     /gene="lpxD"
                     /locus_tag="VEW08_00195"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HYJ39174.1"
                     /translation="MTVTLGELAVRFGCELRGDPAVTVDSVAALSQAGPRSITFLANS
                     KYVAQLAGTRAGAVILDAKSAGDSPVPALVVANPHATYARVATLLHPDPPLHPGVHPT
                     AIAAGATIDPTAEIAAHAYIGEGARIGARCFIGPGSVIERGADIGHDSRFIARVFVGH
                     DVMFGKRCIVQPGAVIGGDGFGFAPEKGTWIKVPQIGGVKVGDDVEIGANTTIDRGAL
                     DDTIIEEGVKLDNLIMIAHNVRIGAHSALAAAVAIAGSSVVGKRCVLAGKVGLSGHVT
                     LCDDVVVLGTSFISHSITRPGVYSSALPSEEAGVWRRIVGRIKRLDSMARRLRAVEKH
                     VGFSAIDKDSSED"
     gene            complement(10689..11252)
                     /locus_tag="VEW08_00200"
     CDS             complement(10689..11252)
                     /locus_tag="VEW08_00200"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HYJ39175.1"
                     /translation="MQVSFRKILLGSLFAATFAMPAAAEIKVGVVDIAKLFEESPQAK
                     VVQDGLRAEFGPRLQQLVAQEQALKARNDKYQKDLATMAADQRTKAEKDLRDSAREIE
                     RKKAELQDDSNAKRQEEMNKLQRLLFGEVREYAKAQNFDIVIAEGVLYATPTVDITAA
                     VLALLQSRAPKPAAGAAPAPAKPPAKP"
     gene            complement(11284..13719)
                     /gene="bamA"
                     /locus_tag="VEW08_00205"
     CDS             complement(11284..13719)
                     /gene="bamA"
                     /locus_tag="VEW08_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HYJ39176.1"
                     /translation="MTIRFATLCAVSAIVLTAPLHAQEGAAFTVGDIRVEGLQRVTEG
                     TVYNYLPINIGDTLSPQRIREAVRALYDTGFFRDVELRRDGNMLIVVVNERPTIESFE
                     LKGNKDIKTEDLGKSLRNVGLAQGKTFDRSVLEDVKSFLQDQYGARGKYAARIDTTVE
                     EVAGNRVKIKIDIVEGKRAKIRQINIIGNHTFKEKDILESFKLKTPNLLSFYKQDDRY
                     SREDLQGDLENLRSFYMDRGYANFEVESTQVAIAPEKDDIFITVNVNEGEVFKVKEVK
                     LAGTFVVPEPLLRRYLLVQPGDTFSRKIITTTQELLQNRLGEDGFAFAKVDPVPTAIG
                     DPANKELSLTFFVDPGNRVYVRHIAFNGVEKINDEVLRREMRQLEGGWLSNAALDRSK
                     QRLERLPYIKKVDSETKPVPGSTDLVDVIYEIEEGPSAQLGGGIGYSESQSFILSGNY
                     ADANFLGTGRRISVDLNSGQYSKVYGVSMTEPYFTPNGVGLTGNVTYRDQTQFVSASS
                     DFSSETIGAGFDIGYPIGENQGLRMGLQYQKSSLLTSVGGSARQSIAWVDANGDSYRE
                     CIDYDTTIPSCDVTLVGTEYNTIELTASWSFDTRNRSLFADRGMRHSFGLAYTLPGVS
                     DVEYYVASYDFIKYIPLFGRFTLQVGADLAYGMDIGDTTALPPYRQFYGGGPETVRGY
                     KESRLGPKDDFGRPFGGNLKVTGRAEVIIPLPQKFQSSARLSLFYDLGNIFSTGNRYV
                     FLGPSGVFAPPVDYSFKYDKLKHSTGVAVQWLAPLGVFRFSYALPLNAYKGDAYLYED
                     EVEQFQFSVGQAF"
     gene            complement(13744..15123)
                     /gene="rseP"
                     /locus_tag="VEW08_00210"
     CDS             complement(13744..15123)
                     /gene="rseP"
                     /locus_tag="VEW08_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HYJ39177.1"
                     /translation="MEIGWSALWFIVAISLLVTIHEFGHFWVARKLGFKVLRFSVGFG
                     KPLYKRVGPAPDYTEFVIAALPLGGYVRMLDERDGTVAPEDLPRAFTRRPPWQRILVL
                     LAGPAANILFAVLVLWGIFWANGVNHLKAVVDRIVVGSPAAAAGLTNGDEIRSIDGEP
                     VLDQVDSTLGLLDAVSDDGEAVMVVRNRLGAERTVTLTISDPDVRHKLTEPYQLYLGL
                     GFEYWTPPFPAVIGEITPGGPADRAGLLVGDEIKAMDGTTMRSFTEVRDYVRARPGKE
                     IVVVVRRGGSDVTRRVTASSDVEEKQTVGRLHFKPAEVSLSYPPEMRTHTVVGPLSAL
                     SYSIGKAWQMTAVQAKFFVRMLTGDVSWKNTSGVIGIATSVGEAAHAGIVTFLMLMVL
                     LSLSLGFLNLLPIPILDGGQIVFQLVEWAKGGPLSDRTYLVGQQAGLLLLVLLMGVAL
                     FNDLSNLNY"
     gene            complement(15133..16335)
                     /gene="ispC"
                     /locus_tag="VEW08_00215"
     CDS             complement(15133..16335)
                     /gene="ispC"
                     /locus_tag="VEW08_00215"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HYJ39178.1"
                     /translation="MKSDGVKGVAILGSTGSVGEHTLDVIARHPDRFRVVALGANRNV
                     EKLADQCLRFSVPYAALADPAAAARLEGELRARKAPTQVIGGVDALEEIAGLPEVDSV
                     MAAIVGAAGLRSTLAAARAGKRLLLANKESLVMAGPLLMQTVRDSGAMLLPIDSEHNA
                     VFQCLPPNSRCGEAPPGVKKILLTASGGPFLDADRARLDRVTPDEACAHPNWVMGRKI
                     SVDSATLMNKGLEFIEACLLFSVRPDQVEVVIHRESIVHSLVEYVDGSMLAQLGAPDM
                     RTPIAHALAWPERVTSGVQSLDLVKVGHLRFEAPDLRRFPALALAQDAALAGGLRPAS
                     LNAANEVAVSAFLAGGLNFARIPAVIESVMATTSGGEIRDLNDVLAADAEARERASSF
                     IRPRAVHA"
     gene            complement(16332..17138)
                     /locus_tag="VEW08_00220"
     CDS             complement(16332..17138)
                     /locus_tag="VEW08_00220"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HYJ39179.1"
                     /translation="MLKERVLTAIVLAAVLLGVMLGLPSVATVWLLTVLVLIGAWEWA
                     GFIGKNTPATRAAFTVAVALALVVCLYFYTSYPDFVLVTVSVAVAWWFIAFLWLCLAP
                     GRVTPVTAALAGFLSLVPCWLALVHVTFTTQSTRWVLFTLALVWAGDTGAFFVGRWLG
                     RVPLAPRVSPKKTWEGALGGIAASGAVAWASAKWLFGLDAWPFVMTCVAVAALSIVGD
                     LTESMLKRAAGLKDSGTMFPGHGGMLDRIDSVTAAAPALVFALIGLKVIT"
     gene            complement(17131..>17763)
                     /gene="uppS"
                     /locus_tag="VEW08_00225"
     CDS             complement(17131..>17763)
                     /gene="uppS"
                     /locus_tag="VEW08_00225"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HYJ39180.1"
                     /translation="RPVRLCIEECARRGVGALTLFAFSSENWQRPTVEVTSLMQLFLD
                     AIDREVDDLHKNKVRLRFIGDRHALSVRLQARMAAGEQLTAANPGLKLQVAVSYGGRW
                     DIVQAARRLAGQVASGALSLDAIDEGLFGRELQLGDMPDPDLFIRTGGDHRISNFLLW
                     NLAYTELHVCETLWPDFDVPDLEAALVAFASRERRFGLTRAQVQEASRRA"
BASE COUNT         3270 a         5767 c         5620 g         3106 t
ORIGIN      
        1 tccgtcgatt tcaatttgag cacgatgcgg cgcgcttctt tctcgcgaag gcgctcgagc
       61 tcgctgacct tgccggcgcg cagcacccag gtgttcgcga attcgtcgaa gcgcagcttg
      121 ccttcgatga gcagcaacgc gtccttcatg aggacctcgc ggtactgctg gtattgctcg
      181 tcgaagaatc ccaccgagat gcggccggag tcgtcgtcca gcaccacctc ggtacgcggg
      241 ccgcgcttgc gcaattcctc gaccactccg gcaattgcca cgggcttgcc accgaagaat
      301 gcgcggccgc cttcggcgct ggcctgaggt ttctcggcgt cgatgtaatc gacgatacgg
      361 ccggtgatga accgcggcag gtcgttcacg aaacgatcga tggggtgacc tgagatggca
      421 cggcccagcg tctcgcgctc ggcacgcaac cgcacgctgt cagtccattc cggttgatcc
      481 ggcgcggtgg cgcgcaccgc ctcgggaacg tgggcagcgc tcaggccgaa aatgtcgtgc
      541 tggccggttt cattcgcctt gctgttctgc tcgcccagcg ccagcgcagc gtcgagacgg
      601 tgcatgagcg tggcgcggtt cgccgcgagg ccatcgagac tgccggattt gatcagcgcc
      661 tcgagcacac gccggttgat cttcgaaaga tcgaggcggc gacacaaatc ttcgaggctc
      721 gtgaaatcgc catgcgcacg gcgctcgttc acgatggact cgaccgcgcc ctgccccacg
      781 cccttgatgg cgccaagacc gtagcgaatg gtcttgtcgt cgaccaccgc gaattcgtac
      841 gcggacagat tcacgtgcgg tggcaggacc gtgagcccga cttccttgcg gcagtcgtac
      901 aacatgccga cgactttgtc ggtgtggtcc atgtccgcgg acagcaccgc ggccatgtac
      961 gaggcggcct cgtgcgtctt gagccacccg gtctggtacg acaacaatgc gtaggcggcc
     1021 gagtgcgact tgttgaatcc gtagccggca aatttttcca tcaggtcgaa gatgtgcgcg
     1081 gcctggccct cgggcacacc gttcttcacc gcgccgctca cgaacttgcc gcgctccttc
     1141 gccatttctt cgggcttttt cttgcccatc gcgcggcgca gcaggtcggc gccgccgagc
     1201 gtatagctcg cgagcacctg cgcgatctgc atgacctgtt cctggtagag gatcacgccg
     1261 taagtgggcg cgagcaccgg ctccagcgac ggatgcagga agtcgatggg cggcgcgtgc
     1321 gggtcgtggc ggctgtgctt gcggtcgatg aagtcgccga ccatgcccga ctcgagcggc
     1381 cccgggcgga acagcgccac cagcgccacg atgtcttcga accggtccgg cttcaggcgc
     1441 cggatcaggt ccttcatacc gcgggattcc agctggaaca tcgccgtggt cttcgcctcg
     1501 gacatgagct ggtacgtggc ctggtcgtcc atcggcaact ggctgatgtc gagcagcgcc
     1561 tcgccggccg cggcgcgccg cgcgttgatg atcttcactg cgcgatcgat gaccgtgagt
     1621 gtgcgcaggc ccaggaagtc gaacttcacg agacccgcgg cttccacgtc gtccttgtcg
     1681 aactgcgtga cgacactgcc acccttgtcg tcggcataca gcggcgcgaa ttccgtcagc
     1741 ttcgagggtg cgatgaccac gccaccggcg tgcatgccgg cgttacgcgt caggccttcg
     1801 agcgacatgg cgaggtcgat gaggttcttg acctcctcgt cttccttgta gaggcgcttg
     1861 agctcgggtt ccttttcgag cgcgtcggcc agcgtgatgc cgagctcgaa cggaatgagc
     1921 ttcgcaatct tgtcgacgta gccgtaaccc atgccgagca cgcggcccac gtcacgcacc
     1981 acggccttcg ccgccatggt gccgtaggtg atgatctgcg agacacgctc gcggccgtat
     2041 ttctgcgaga cgtactcgat gacacgatcg cggccgtcca tgcagaagtc gatgtcgaag
     2101 tcgggcatcg acacgcgttc gggattcagg aagcgctcga acagcaggtc gtacttcagc
     2161 ggatcgatgt cggtgatccc caggctgtac gccaccagcg aaccggcgcc cgagccgcgc
     2221 cccggcccca ccggcacgcc gttggcgcgc gcccagcgga tgaagtcggc gacgatgagg
     2281 aagtaccccg ggaatcccat ctggcagatc acgccgagct cgcgctgcag gcggtcgcgg
     2341 tatacctgga tgcgtgccag ctgcgacgca tcgaacactt tctcgcgttc cgcgaaacct
     2401 cgctcggatt cggcgcggat atgcgcgtcg acggtcgtgc cttcgggcag cggatagtta
     2461 ggcaggcgcg attcgccgag cttgagcggc agcgaacacc ggcgggcgat ttccaccgag
     2521 ttcgccagcg cctcgggaat atcggcgaac agcttcgcca tctcgtcggg cgagcgcagg
     2581 tactgcgcct cggtgtaacg gcgttgccgt cccggatcgg caagttgcgc gccatcggaa
     2641 atgcagacgc gcgcctcgtg cgattcgaag tcactggcgg cgaggaagcg cacgtcgttg
     2701 gtggcgacca cgggaatgcc ggccttctgg ctcaagctga cggtcctggc gaccatcgat
     2761 tcgtcatcgg cgcgcccgag gcgttgcagc tcgaggtagt agcgatcgcc gaaaagctcc
     2821 gcccattcgc gcgcgaggac cagtgcgtcg gcctcgcggc cattggcgat ggcgcggccg
     2881 acatcacctt cggcaaatcc cgagagtgca atgagcccgg tggtggtttc cggtgtcagc
     2941 cagctgcgat cgatgagagg cacgccgcga tcctggcctt cgagccaggc gcgcgtgacg
     3001 agattcgtga cgttctggta gccacccggg tccttgcaca gcagcgtcag ccggttgggc
     3061 ggcaggcgtt caccggcgcg catctggacg tccacgccga tgatgggttt gacgccgcac
     3121 ttcaatcctt cgcggtagaa cttcaccatc gcgaagagat tgttctggtc agtgacggcc
     3181 accgccggca ttccgagcgc caccacgcgt tccaccagct ccgggattcg caccacgctg
     3241 tcctgcaacg agaactcggt gtgcaggcgg aggtggacga aagcggcggc actcttcatg
     3301 gcgcgcgagt ttacacacga acgtgcgcgt taatcgggtc ggcaaaagga ttcaaatact
     3361 tgcaggccct cgttcgagca gccgcaagga cgacctggcg atcaccctat tttccggccg
     3421 gcgatggcct gcgataccgc ggcgcagggc ccgccttgcg tcgccaaagt gggagccggg
     3481 tcacactcac gaccccgttt gaatgcggct ctggaaaatg tcgccgattc gtaaggaaac
     3541 cccggcggga aacctgtgaa cttgtgagtc gtttcaacgg attactgccc aagaacaccg
     3601 ataatttcgc cctcgttcgc accatattcc ggggacccga aatggcgtat cgcaacctca
     3661 ggctcgccgg cactctcatg ctgatcgcct gtggcgtttc agcgctcgca tcggcacaga
     3721 cgccatcgac tctctatgcg cgtctcggtg gaacacctgt ggtatccgca tttgtcgcgg
     3781 acgcgctcga caaggtcgtc gcgagtccgc aaatgaaccg aaccctcgac aaggcaagtc
     3841 tgcaactcat gaaggatttg ctgactgaac ggatctgcac gctgacgggc ggtggctgca
     3901 cccacggtgg cgacgtgatg cgcgaggtgc gcacggaccc ccacatcagc ggcgcggaat
     3961 tcaccgggct gatcgaggcg atgcgcgagt cgatgcgtgc ccacgacgta ccgttggcgg
     4021 cccgcaacca attactggaa atcctcatcc cgatgaacca ggacgtagcg agactttgaa
     4081 accgtgaacg cgatgcggga tacggtgccc tgatgcgcat acagcgcctg caaacccgca
     4141 tcatcgtgtt gttcgtggcg ctgctgaccc tggtgcaggt cgccgcattc tggttcgtga
     4201 acgcggccaa ttcgcgcaac gcggaagaca aggtcgccga agaactcaac gtcggtcaac
     4261 gcgtgttcgc gcgcctgctc gaccagaacg cggagaaact caagctctcg gcgcgcgtgc
     4321 tcgcggcgga cttcgcgttt cgcgaagcca ttgcgacgca cgacaccggc accatcgcct
     4381 cggcactcgc caaccatggc gcgcgcatcg gcgctgaagc catggtgttc gtcgatctgc
     4441 agggccaggt catctccgac acgctgagta ccggcgccac cgcacgcccg ttcgaatatc
     4501 cggcactgct caagtccgac aggaacgccg ccagcgccag catggaagtg ctcgacgatc
     4561 gcgcgttcca gctcgtagcc gtgccggtgc tggcgccact gcccataggc tgggtcgtca
     4621 tggggtttgc ggtggacgat tcgctggcca gcgatctgcg ccagctgacc gaactcgagg
     4681 tgtcgttcac gctcgacggc cagccagacc ggcgcatgct cgcatccacg ctggggctgc
     4741 gcgaacagca ggaactcctg aggcatatgc catcgcgatc gaacagcgtg acgcgcatgg
     4801 tcatggacat tcgtggggtc gatcaccagg cgctggtgat tccgctcgac gacaacgaca
     4861 acgcccgcat cgtcgccgta ttgcaccgct cgctgggcga cgcgctggcc gcattcgagc
     4921 gcctgcgcaa tacgctcttc gtcctcgccg cgctgagcct cgcgatgtcg atcgtgggaa
     4981 gcgtcgcggt ggcgcgcaac atcacgcggc cgctcgaaac cctggcgacc gcggcggcac
     5041 gaatcgaaca gggcgactac agcgctccgg tcgatctgcg gcgcgccgac gaaatcggcg
     5101 tgctggcttc gagcctgaat cacatgcgag gcagcatcgc cgatcgcgaa aggcgaattc
     5161 tcaagctggc ttacgaagac ccgctcaccg atctcgccaa tcggtcgcgt ttctccagcg
     5221 atctcgagct cgccatcgcg caggcggcat tgcgcgatct caagctcacc atcctcatga
     5281 tggacctgga ccgcttcaag tacgtcaacg acacgctcgg tcacggcgtg ggcgaccacg
     5341 tgttgcgtga agtgagcgcg cgcctgcaac gcacggtgac cgacgccgag tgcatcgcgc
     5401 ggctgggcgg cgacgaattc gccatcctcg tgcgacacgc cgacgacgac ttcgccgaga
     5461 ccgcgcgcaa gatcatcgca gcactcgaag cgccgattct ctacgagggt cagcctctcg
     5521 acgtgggcac cagcatcggc attgcccact tccccgagca cgggcgtgac gcacagaccc
     5581 tggtgcgcaa cgccgacatc gcgatgtacg cggccaaacg aaacaagacg ggttttgcca
     5641 cgtatgagca acactacgac accagccagc aggaacatct ctcgctgctc tccgagctac
     5701 gccgcgcggt ggaacgcaat gagctgcgcc tgtactacca gccgaaggtc tcgctgcact
     5761 cctcgcacgt gagcgccgtc gaagcattga tccgatggga acatccgacc cgcggcctgg
     5821 tgccgccttg ccaattcatt ccgttcgccg aacacaccgg ctacatcaag atcctgaccc
     5881 gatgggtgat ccgtgaagcc gtgcgccagt gcggtgaatg gctgcgctcc ggcctcgcgt
     5941 tgcaggtatc ggtgaacatc tcggcgcgcg acctcatgaa ccgtgaattg cccgagcacg
     6001 tcgccgaatt gctcgccgag catgatgtga cgcccgggct gctttgcctg gagatcaccg
     6061 agagcggctt catggaggat ccgacgcatg cgcagaaggt cgtcgaccgg ctcgcggagc
     6121 tcggcgtcaa actatccatc gacgactacg gcaccggcta ttcgtcgctg tcgtacatca
     6181 tgaagctgcc agtgcaggaa ctgaagatcg atcagtcctt catcgcgcgc atggcgacgg
     6241 acgaggaaat ttcgaccatc gtgcggtcga ccatcgacct cggccacaac ctggggctcc
     6301 aggtggtcgc cgagggcgtc gaggatgcgg ccgcgtggga gatgctgcga acgctcggct
     6361 gcgatgacgc gcaaggatat ttcatgagcc agccgctcga gccacaggcg ctcgcgaact
     6421 ggattcgtgc gaacaagggc aattttgcct gcgattctca ggggctgcgc cccacgtcgg
     6481 aaaacgccgc cgaagcggcg cattctcagg ggctgcgccc cacgtcggaa aacgccgccg
     6541 aagcggcgtc gtccaataag tagaagaacg tcctagccga agtcggcttc ttcaatacag
     6601 tcgctcaagc gcacgggtgc gaaactccgg cggtgcaacg ggctcgggcc cagcgcattc
     6661 aacgccgcca catgcgccgg cgtgggataa cccttgtgcc cggacagcga aaaacccggg
     6721 tagcggtggt cgagatcacg catcagtgcg tcgcgcgtgg tcttcgcgag aatggatgcc
     6781 gcgctgatcg acggcacggt ggcgtcgccc ttgatcaccg cctcgaacag gcattcgaac
     6841 ccgaggccgt ccgcgcacgg acaacgatta ccgtcgatga aaacctgttg tggcggcacg
     6901 gccagaccca tcaatgcgcg ccgcatggcg agcatggtcg cctgcaggat gttgatgctg
     6961 tcgatttctt ccgcgtcggc ccatgccacc gcgtaacaca gtgcccgatt gcgaatcagg
     7021 atggcgaggc tttcacgctc ttcgggttcg agcgttttcg agtccgcgag cccgcggatg
     7081 ggacgcgccg gattcagaat gaccgccgcg gcaaccacgg gtccggcgag cggcccgcgc
     7141 cctgcctcgt caacaccggc cacgcgcggg cgcacacgca gtttcatgcc ggcatcgccc
     7201 gggtcgccag aagcgcggcc acttcgtccg ccgcacggtc ggcgccgtcg caacgcagcc
     7261 gggtgtggat cgcctcgaat tcctgctgta ctcgtgcgac gtccgcggga tgttcgatcc
     7321 agtgcgccag tgcattggcg agattcgccg gcgacgccgc ttcctggaag aattccggca
     7381 ccagctcctt gcccgccagc aggttgggct gcgagaaatg tttgatcttc acgaggccga
     7441 gcgttcgaag gagaaacgcg gtgatggcgc ccaaccggta ggcgaccacc atcggccgtt
     7501 tgaaaagcgc cgtctcgagc gtcgcggtgc ccgacgccac cagcaccacg tccgccgccg
     7561 cgagtacgcg acgtgcctgg ccgtcgagca gtcgcacctg cgcatggggc gcaagcgctg
     7621 cgcagcgcgc ggcaaacagg tcacgcagaa cgggcgtcac catgggcgcg atgctgacca
     7681 gaccgctgaa gcgagttgcc aggagctcgg ccgcgcctgc gaaaacgtcg gccagctttt
     7741 cgacctcccc gcgcctgctg ccgggcaaca cggcgagcac gcgcactgag ggatcgagtc
     7801 cgagttcggc gcgcgccgcg gcgcgatcgg cgaccatcgg aatctgatcg gcaagcgggt
     7861 gaccgacgaa tgccgcgcgc acaccgtgct cgcgataaaa atccggttcg aagggaaaca
     7921 ggcacagcac caggtccacc gaggcgccga tggtgcgcac gcgtccctgt cgccaggccc
     7981 agacctgcgg gctcacgtat tgcacggtag cgaggccctc tcgcttcagt tgcctggcga
     8041 gccccaggtt gaattccttg taatcgacgc cgatgaaaac gtcgggccgc cactcgagga
     8101 tccgccgccg caattcggca cggagccgca tcaggcgcgg caggtgtgtg agcacctcgg
     8161 tgagtcccat gaccgccagc tcttcgatgt cgtgccaggc tgcgcaaccg gcggccttca
     8221 tcctcgggcc ggcaacaccg gcgaattcgg cttgcgggta ttggcggcgc aatgcctcga
     8281 tgagcgcggc acccagcgtg tcaccggatg tttcacccgc gaccagtgcg atgcgcaaag
     8341 gcgtctggcc ccttatcgca cgaggctgcg cgtggactcg ccgatgaaat cgacgaagat
     8401 cttcagctcc ggctgcgtgg ccgcacgcga ctgaagctcg gccactgcgt cgaccagctt
     8461 gaggtccgag cgatagagga cgcgataggc attcttgaga ttgcggatct gctcgggagt
     8521 gaagccgcgg cgcttcaggc cttccgagtt gatcgaatgc ggcaccgcgg gcgtgccgac
     8581 ggccatgacg tacgggggca catcacgcgt gaccgcggcg ttgttgccga tgaaggcatg
     8641 cgcgccgacc ttggtgaact ggtgggtgcc ggcataaccg cccatgatca cccagtcgcc
     8701 gagctccacg tgcccggcca gcgtcgcgta gttagccatg acgcacttgc tgcccacgcg
     8761 acagtcgtgc gccacgtgcg agtaggccat gaacaggttg tcgttgccga tgatggtgac
     8821 gccgccgccc tgcgcggtgc cgcgattcat cgtggtgaat tcgcgaaaga cattgcgatc
     8881 gccgatttcc aggcgcgtgg gctcgccctt gtacttgaga tcctgcggtg catcaccgat
     8941 ggacgcgaac tggaagatct tgttgccggc gccgatggag gtgggcccgt tgacgaccgc
     9001 gtgcgggcct atccacgtgc cggcgccgat ggtgacgccg tcgccgatga cggtgtacgc
     9061 gcccaccgtc acgtcggacg caagttccgc cttaggagaa acgatggcgc ggggatcgat
     9121 gccgctcacg ccgccggtgc ctttgccgaa gtcttgttgg tttcgggcgc gaccataagt
     9181 tctgcggaag ttacctcatc atcccccacg tatgccacgg cggagaactt ccagatgccg
     9241 cgcatggagc gttcgagtct ggcggtgagg atgagctgat cgccaggctc cacgggcttg
     9301 cggaagcgcg ccttgtcgat accgacgaag aagaatcgcg tcgaatcgtc cggcacgacg
     9361 tccgcggtca tgaacgccag caagcccgcg gtctgcgcca gcgcctcgat gatcaacacg
     9421 cccggcatca ccggccgatg cgggaaatgc ccggaaaaga acggctcgtt ggccgtgatg
     9481 ttcttgatcg ccctgatgct ctcgcccttg acgcactcca gcacgcggtc gacgagcaga
     9541 aaggggtagc ggtgcggcag cagccgcctg acgcccatgt ggtcgaggga aatcgactcg
     9601 ctcattagtt agtcctcgga agaatccttg tcgatggcgc tgaaacccac gtgcttctcc
     9661 acggcgcgca accgcctggc catcgagtcg agccgcttga tgcgcccgac gatacgccgc
     9721 cagacacccg cctcctcgct cggcagcgcg gaagaataca cgcccggcct ggtaatggag
     9781 tgagagatga aggacgtgcc cagcactacc acgtcatcgc acaacgtgac gtggccgctc
     9841 aggcccacct tgccggcgag cacgcagcgc ttgcccacca cgctgctgcc ggcgatcgcc
     9901 acggcagcgg ccagtgccga atgcgcaccg atgcgaacgt tgtgcgcgat catgatgaga
     9961 ttgtcgagct tgacgccttc ctcgattatc gtgtcgtcga gcgccccgcg gtcgatggtg
    10021 gtgttggcgc cgatttcgac gtcgtcgccg accttcacgc cgccgatttg cggcactttg
    10081 atccaggtgc ccttttcggg cgcaaaaccg aagccatcgc ccccgatgac cgcgcccggc
    10141 tgcacgatgc agcgcttgcc gaacatcacg tcgtgcccga cgaatacgcg ggctatgaag
    10201 cgtgaatcgt gaccgatgtc ggcgccgcgc tcgatgaccg acccgggacc gatgaaacac
    10261 cgcgcgccga ttcgcgctcc ctcgccgatg tacgcgtgtg cggcgatttc cgcagtcgga
    10321 tcgatggtgg cacccgccgc gatcgcagtc gggtggactc cgggatgcag gggcggatcc
    10381 gggtgcagca gggtcgccac gcgcgcgtac gtggcgtgcg gattggcaac gaccagcgca
    10441 ggcacgggcg agtcccctgc cgatttcgcg tcgagaatga ccgcaccggc gcgcgtgccg
    10501 gcaagctgcg cgacgtactt ggaattggcg aggaaggtga tcgaccgtgg gccggcctgc
    10561 gacagcgcgg cgacggaatc caccgtcacc gcggggtcac cgcgcagctc acagccaaat
    10621 cgcaccgcca gttcgccgag cgtcactgtc atggcggccg gccgttagcg tccctggcgg
    10681 ggcgcgcctc agggcttcgc gggtggcttc gcgggtgccg gtgccgcgcc tgcggccggc
    10741 ttcggagcac gcgactgcag taatgccagg accgcagcgg tgatgtcgac cgtgggagtg
    10801 gcgtaaagca cgccttccgc gatcacgatg tcgaaattct gtgccttggc gtactcgcgg
    10861 acttcaccga acaacaggcg ctgcagcttg ttcatctctt cctggcgctt ggcattcgaa
    10921 tcgtcctgca gctcggcctt cttgcgctcg atttcgcggg cgctgtcacg cagatccttc
    10981 tcggccttgg tgcgctggtc ggcggccatc gtcgccagat ccttctggta cttgtcgttg
    11041 cgggccttca gtgcctgctc ctgagcaacg agctgctgga ggcgcgggcc gaactcggct
    11101 cgcaggccgt cctgaacgac tttcgcctgt ggcgactctt cgaacagctt ggcgatatcg
    11161 acgacgccga ccttgatctc ggcggcggcg ggcatggcga acgtcgccgc gaacagcgaa
    11221 cccaaaagaa tcttgcggaa ggaaacctgc actgaactta cctctttata aagaactggc
    11281 cccttagaaa gcctggccaa ccgagaactg gaactgctcc acctcatctt catagagata
    11341 ggcgtcgccc ttgtacgcat tcaggggcaa ggcgtagctg aaacggaaca ctcccagcgg
    11401 tgcaagccat tgaacggcaa cgcctgtcga gtgcttcaat ttatcatact tgaacgagta
    11461 atcgaccggc ggagcgaaca ctccggaagg tccaaggaag acataccggt tacccgtcga
    11521 gaagatgttg cccaggtcgt agaacaagga gagccgggcc gacgactgga acttctgcgg
    11581 cagagggatg atgacttccg ccctgcccgt gaccttcagg ttgccgccga acggacggcc
    11641 gaaatcgtcc ttgggaccca ggcgactctc tttatatccg cgcaccgtct ccggtccgcc
    11701 gccgtagaac tgccgataag gcggcagcgc cgtggtgtcg ccgatgtcca taccgtaggc
    11761 gagatccgca cccacctgca gtgtgaaccg gccaaacagc gggatgtact tgatgaaatc
    11821 gtagctcgcc acgtagtact cgacgtcgct gacgccgggc agcgtgtagg cgaggccaaa
    11881 cgaatgccgc atgccgcgat cggcaaacaa cgaacgattg cgggtgtcaa agctccacga
    11941 agcggtgagt tcgatcgtgt tgtattcggt gcccacgagc gtgacgtcgc acgacggtat
    12001 cgtcgtgtcg tagtcgatac attcgcggta ggagtcgccg ttcgcgtcca cccaggcgat
    12061 ggactggcgt gcgctgccgc cgacgctcgt gagcaggctc gatttctggt actgcaggcc
    12121 catgcgaagg ccctggttct cgccgatcgg ataaccgatg tcgaaacccg cgccgatcgt
    12181 ctccgacgag aaatccgacg aggccgacac gaactgggtc tggtcgcgat atgtgacgtt
    12241 gccggtgaga cccacgccgt taggcgtgaa atatggctcg gtcatcgaaa ctccgtacac
    12301 cttgctgtac tggccggagt tgagatcgac cgatatgcgc ctgccggtac cgaggaaatt
    12361 cgcatcggcg tagttgccgc tgaggatgaa cgactgggac tccgagtagc cgatgccgcc
    12421 acccagctgt gcggacgggc cctcctcgat ttcgtaaatg acgtcgacca ggtcggtgga
    12481 accggggacc ggcttggtct ccgaatcgac cttcttgatg tacggcaggc gttcgagacg
    12541 ctgcttggag cggtcgagcg cagcgttcga aagccagcca ccctcgagct gccgcatttc
    12601 gcggcgaagc acttcgtcgt tgatcttctc gacgccgttg aacgcgatgt ggcgcacgta
    12661 aacgcggttg cccggatcga cgaagaacgt gagcgacagc tctttgttag ccgggtcgcc
    12721 tatggccgtg ggaaccggat cgactttcgc gaacgcgaag ccatcttctc ccaggcggtt
    12781 ctgcagcagc tcctgggtgg tggtgatgat cttgcgcgag aacgtgtcac ccggctgtac
    12841 cagcaggtag cgccgcagga gcggttccgg caccacgaac gtcccggcga gtttcacttc
    12901 cttgaccttg aaaacctcgc cttcgttgac gttcaccgtg atgaagatgt cgtccttttc
    12961 gggcgcgatg gcgacctgcg tcgattcgac ctcgaagttc gcgtagccgc ggtccatata
    13021 aaaggagcgc aggttttcga ggtcaccctg caggtcctcg cgcgaatagc ggtcgtcctg
    13081 cttgtagaac gacagcaggt tcggcgtctt gagcttgaag gactcgagga tgtccttttc
    13141 cttgaacgtg tgattgccga tgatgttgat ctggcggatc ttggcgcgct tgccttcgac
    13201 gatgtcgatc ttgatcttga cccggttacc cgcgacttct tcgaccgtcg tatcgatgcg
    13261 tgccgcgtat ttgccgcgcg cgccgtactg atcctgcagg aacgacttca cgtcctcgag
    13321 cactgaacga tcgaaggtct tgccctgcgc caggcccacg ttgcgcagcg acttgcccag
    13381 gtcttcagtc ttgatgtcct tgttgccctt gagctcgaag ctttcgatgg tcggacgctc
    13441 gttgaccacc acgatcagca tgttgccatc gcggcgcaat tccacgtcgc ggaaaaaccc
    13501 ggtgtcgtac agcgcgcgca ccgcttcgcg aatgcgctgc ggcgagaggg tgtcaccgat
    13561 gttgatcggt agatagttgt agaccgtgcc ttcggtgacg cgctgcagac cctcgacgcg
    13621 gatgtcgccg acggtgaatg cggccccctc ctgagcatgc aacggggctg tcagcacaat
    13681 tgcactgacg gcacaaagag tcgcgaaccg tatggtcaag tcagtcccct gggaaattcg
    13741 ttcttaataa ttcaagttgg acaggtcgtt gaacagcgcc acgcccatga gcagcaccag
    13801 cagcagcagt ccggcctgct gcccgaccag gtaggtgcgg tcggacagcg ggcctccctt
    13861 cgcccactcg acgagctgga agacgatctg tccgccgtcg aggatcggta tgggcagcag
    13921 gttgaggaag cccaacgaca atgacagcag caccatcaac atgaggaaag tgacgatgcc
    13981 cgcatgcgcg gcttcgccga ccgacgtggc gatgccgatc acacccgagg tgttcttcca
    14041 cgacacatcg ccggtgagca tgcgcacgaa gaacttcgcc tgcaccgccg tcatctgcca
    14101 ggccttgccg atcgaatacg aaagcgcgct cagcggcccc accacggtgt gcgtgcgcat
    14161 ctcgggtgga taggaaaggc tgacttccgc aggcttgaag tgcaaacgcc cgactgtctg
    14221 tttctcttcc acgtcgctgg acgcggtgac gcggcgggtc acgtcgctgc cgccacgccg
    14281 gacgacgacg acgatttcct ttcccgggcg ggcgcgcacg tagtcgcgaa cctcggtgaa
    14341 acttcgcatc gtcgttccgt ccatcgcctt gatctcgtcg cccaccaaaa ggccggcgcg
    14401 gtcggccgga ccccccggcg taatctcgcc gattacagcg ggaaacggcg gcgtccagta
    14461 ttcgaacccc aggcccaggt agagctggta aggctcggtg agtttgtggc gcacgtccgg
    14521 atcggaaatc gtgagcgtga cggtacgctc cgcgccgagg cggttgcgca ccaccatgac
    14581 cgcttcgcca tcgtcgctga ccgcgtctaa caagccgagc gtcgagtcca cctggtcgag
    14641 caccggttcg ccatcgatgc tgcggatttc atcaccgttg gtgagacccg cggcggccgc
    14701 cggcgagccg acgacgatcc ggtccaccac cgccttcagg tgattgacgc cattcgccca
    14761 gaaaatgccc cacagcacga gcaccgcgaa gaggatgttt gccgcaggac ccgccagcaa
    14821 taccaggatg cgctgccagg gcggccgcct ggtgaaagcg cgcggcagat cttctggcgc
    14881 caccgtgccg tcgcgctcat cgagcatgcg cacgtatcca ccgagaggca gcgcggcgat
    14941 gacaaattcc gtgtaatcgg gcgcggggcc gacgcgcttg tagagcggct tgccgaagcc
    15001 caccgagaaa cgcagcacct tgaagccgag tttgcgcgcc acccagaaat ggccgaactc
    15061 gtggatcgtc accagcagac tgatggcgac gatgaaccaa agcgcgctcc agccgatttc
    15121 catcgagctc ccttaggcgt gcacggcacg aggccggatg aatgaggaag cgcgttcgcg
    15181 cgcttcggcg tcggccgcca gcacatcgtt gagatcgcgg atttcgccac ccgatgtggt
    15241 tgccatcact gactcaatga ctgccggaat tcgtgcaaag ttcaatccac cggcgagaaa
    15301 ggccgatacc gcgacctcgt tggcggcatt aagtgatgcc ggtcgcagtc cacccgcgag
    15361 ggctgcgtcc tgcgcgaggg ccagtgccgg gaatcgtcgc aggtcggggg cctcgaatcg
    15421 caggtgcccc accttcacga ggtcgaggga ttgtacaccc gaggtcaccc gctccggcca
    15481 cgccagcgcg tgcgcgatgg gcgtgcgcat gtcgggagcg cccagctgcg cgagcatcga
    15541 accgtcgacg tattcgacca gcgaatggac gatgctctcg cggtggatca ccacctcgac
    15601 ctggtccggg cgcacgctga acaacaggca ggcctcgatg aactcgagtc ccttgttcat
    15661 gagcgtggcg gagtcgaccg aaatcttgcg acccatcacc cagttgggat gcgcgcaggc
    15721 ttcatccggt gtgacgcggt cgaggcgcgc acgatcggcg tcgaggaacg ggccgcccga
    15781 ggcagtcagc agaatttttt tcacgcccgg cggcgcctcg ccacaacgcg aattgggcgg
    15841 aaggcactgg aacacggcgt tgtgttcgct gtcgatgggc agaagcatgg caccggaatc
    15901 gcgcacggtc tgcatgagca gcgggcccgc catcaccagc gactccttgt tggctaacaa
    15961 caagcgcttg ccggcgcgcg ccgcggcgag cgtggaccgc agtcccgcgg ccccgacgat
    16021 ggccgccatc acgctatcca cctccggcaa cccggcgatc tcctcgagcg catcgacgcc
    16081 gcctatcacc tgggtcggcg ccttgcgcgc acgcagctca ccctcgaggc gtgctgccgc
    16141 ggcaggatcc gcgagcgcgg cgtatggaac gctgaatcgc aggcattggt cggcgagttt
    16201 ttcgacgttg cggttcgcac cgagagccac gacgcggaaa cgatcggggt gacgcgcgat
    16261 cacgtcgagc gtgtgctccc cgaccgaccc ggtggagccc agaatggcga cacctttcac
    16321 tccatcactt ttcatgtgat gaccttgagg ccgatgagcg cgaataccag cgccggcgcc
    16381 gcggcggtca cgctgtcgat gcgatccagc atgccgccgt ggccggggaa catcgtgccg
    16441 ctgtccttga ggccggccgc gcgcttcaac atgctttccg tcaggtcacc gacgatcgag
    16501 agcgcggcca cggcgacgca ggtcatcacg aacggccagg cgtccagccc gaacagccat
    16561 ttcgccgaag cccaggcgac ggcgccgctc gccgcgattc cgccgagcgc gccttcccag
    16621 gtcttcttgg gcgacacgcg cggcgccagc ggcacacggc ctaaccaccg ccccacgaaa
    16681 aacgcgccgg tgtcgccggc ccagacgagc gcgagcgtga acaggaccca gcgcgtgctc
    16741 tgcgtcgtga acgtgacgtg caccagcgcc agccagcaag gcacgagcga caggaatccc
    16801 gccagcgctg ccgtaaccgg ggtaacacgg cccggcgcca ggcagagcca cagaaaagcg
    16861 atgaaccacc acgccaccgc gaccgacacg gtgacgagca cgaagtccgg gtacgaagta
    16921 tagaaataga ggcagaccac cagcgcgagc gcaaccgcca ctgtgaaggc cgcgcgcgtt
    16981 gccggggtat tcttgccgat gaacccggcc cactcccacg cgccgatcag cacgagcacc
    17041 gtgagcagcc acacggtcgc cacggatgga agaccgagca tcacgccgag cagcaccgcg
    17101 gcgagcacga tggcagtgag gacgcgttcc ttaagcacgg cgcgatgcct cctgcacctg
    17161 tgcacgggtc aggccgaagc ggcgctcccg gctcgcgaat gccacgagcg cggcttccag
    17221 gtccggaacg tcgaaatcgg gccacagcgt ttcgcagacg tgcagttcgg tatacgcgag
    17281 gttccacaac aggaagttgc tgatgcggtg atcaccaccg gtgcgaatga aaagatccgg
    17341 atcgggcatg tcaccgagct gcagttcccg cccgaaaaga ccttcgtcga tggcgtcgag
    17401 tgacagcgcg ccgctcgcga cctgccccgc gaggcgccgt gccgcctgca cgatgtccca
    17461 tcggccgccg taactcaccg ccacctggag cttcagcccc ggatttgccg ccgtgagctg
    17521 ttcgcccgcg gccatgcgcg cctgcagcct gacgctgagt gcgtggcgat caccgatgaa
    17581 tcgcaaccgc accttgttct tgtggagatc atcgacctcg cgatcgatgg cgtcgaggaa
    17641 cagctgcatc aaactagtca cttcgaccgt gggccgttgc cagttctcac tcgagaacgc
    17701 gaacagcgtg agcgcaccga caccgcgccg cgcgcattct tcgatacaca ggcgcaccgg
    17761 acg
//
LOCUS       DASQJS010000006         3365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2203574, whole genome shotgun sequence.
ACCESSION   DASQJS010000006 DASQJS010000000
VERSION     DASQJS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2203574"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VEW08_00230"
     CDS             complement(<1..499)
                     /locus_tag="VEW08_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYJ39181.1"
                     /translation="MKSFIQMTLAAAAAALLVACSGGESTSAPGDGTKPIVLGFSQIG
                     AESEWRTANTTSIQSAAPEAGITLRFSDAQQKQENQIKALRSFIAQRVDVIAFSPVVE
                     TGWETVLREAKAAKIPVILTDRAVEVSDASLYLSLIGSDFVEEGRKAGRWLLENMKDV
                     QGDINI"
     gene            complement(539..1738)
                     /gene="araD1"
                     /locus_tag="VEW08_00235"
     CDS             complement(539..1738)
                     /gene="araD1"
                     /locus_tag="VEW08_00235"
                     /inference="COORDINATES: protein motif:HMM:NF040903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraD1 family protein"
                     /protein_id="HYJ39182.1"
                     /translation="MNIAFIADGREQHELTRLEPCADKPGRSRRHLVVFPRPRHLYTD
                     EYSSVAIPGLLDIHTNLMIHRLRLIQFLEQSGQPRVGWVTDDGATVGSVGSFKSTYEL
                     AQAASSAAEPLDAFASKQAAKPTISYTALLQERRVLPPLLHPDPARCLVSGTGLTHYG
                     SASTRDQMHKKLAAESLSDSMQMFKWGVEGGRPQGGMGGEPGAQPEWFYKGDGDSVIA
                     CGQALSSPDFALDGGEEPEIVGLYLIAPDGTPQRLGFAIGNEFSDHVTERKNYLYLAH
                     SKLRPCAIGPELRTGVPPVHMEGMSRIRRGEAIVWERPFVTGEANMCHSLANLEYHHF
                     KYQAHRRPGDVHLHFFGTATLSFADGFAAQPGDVFEVALPALGAPLANPLARTVGGFT
                     FGGVRAL"
     gene            1619..2614
                     /locus_tag="VEW08_00240"
     CDS             1619..2614
                     /locus_tag="VEW08_00240"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HYJ39183.1"
                     /translation="MPGTGEHNKMSTTTPWFIRARLKTRQLMLLTAICDEGNIHRAAE
                     VLNMSQPAASKLLKDLEDMLGVSLFERQPRGMRPNWYGETMIRHARIALSSLREAGQE
                     IDALKAGLSGHVSLGAITGPALSLIPQAIMIVARENPKLRVELHVESSNVLIEQLAQG
                     KIDIMIGRLFERQEKTGLRYERLAEEPVSAVVRPGHPLLNVAGLSLGAVEHKPWIVPP
                     AGSVLRHRFDLMFSEAGFAPPTQLIETASLLFVTKMLQQTDFIAVVPTEVARYYATYG
                     MVALLPMQLSCTMDAFGIITRTDWLLSPAATVLLRALKTSAVSSYGCTLDMKNLS"
     gene            2710..>3365
                     /locus_tag="VEW08_00245"
     CDS             2710..>3365
                     /locus_tag="VEW08_00245"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HYJ39184.1"
                     /translation="MDVPKKRKRRSQDWFGREGKDGFLHRSWIKNQGYPDDLFDGRPV
                     IGICNTWSELTPCNGHFRELAEFVKRGVYEAGGFPLEFPVMSLGETQLRPTAMLFRNL
                     ASMDVEESIRGNPLDGVVLLMGCDKTTPSLLMGAASCDLPTIGISGGPMLSGQFRGKQ
                     IGSGTGVWQMSEDVRGGRMSQEEFTAAESCMHRSKGHCMTMGTASTMASMVEALGVAL
                     P"
BASE COUNT          623 a         1023 c         1086 g          633 t
ORIGIN      
        1 cgatattgat gtcgccctga acgtctttca tgttctcgag cagccagcgt cccgccttgc
       61 ggccttcttc gacgaagtcg gagccgatca gcgacagata aagagaagcg tccgagacct
      121 cgaccgcgcg atcggtgagg atcaccggga ttttcgcggc cttggcttcg cgcagtaccg
      181 tttcccaacc ggtttcaacc accggcgaga aggcgatgac gtcgacgcgc tgggcgatga
      241 acgagcgcag tgccttgatc tggttttcct gcttctgctg cgcgtccgag aagcgcagcg
      301 taatgccggc ttcgggagcg gcgctctgga tggaagtggt gttggcggta cgccattcgc
      361 tttcggcgcc gatctggctg aacccgagca cgatgggttt ggtgccatcg ccgggcgcgg
      421 aagtcgattc gccgcccgaa catgccacga gcagggccgc ggctgcggcg gccagcgtca
      481 tctgaatgaa gctcttcatc gttccccctt gaagtagtaa gtcctgtgca gagccgccct
      541 agagtgcgcg aacgccaccg aaggtgaaac caccgaccgt gcgcgccagt ggattggcca
      601 gcggagcgcc cagtgccggc agggcgactt cgaagacatc gcccggctgg gcggcgaacc
      661 cgtccgcgaa actcagggtg gcggtgccaa agaaatgcag gtgcacgtcg cccggtcggc
      721 ggtgcgcctg gtatttgaaa tggtggtact cgagattggc cagcgaatgg cacatgttgg
      781 cttcaccggt gacgaaaggc cgttcccaca cgatcgcttc gccgcggcgg atgcggctca
      841 tgccttccat gtgcactggc ggcacgccgg tgcgcagctc aggtccaatc gcgcagggcc
      901 gcagcttcga atgggcgaga tagaggtaat tcttgcgctc ggtgacgtgg tcgctgaatt
      961 cgttgccgat ggcgaacccc agacgctgcg gggtgccgtc aggcgcgatg agatacaggc
     1021 cgacgatctc cggttcctca ccgccgtcca gcgcaaagtc gggcgaagac agcgcctggc
     1081 cgcaggcgat gacgctatcg ccatcacctt tatagaacca ctcgggctgg gcaccgggct
     1141 cgccgcccat gccgccctgg ggacggccgc cttcaacgcc ccacttgaac atctgcatgg
     1201 aatcagagag cgactcggcg gcgagtttct tgtgcatctg gtcgcgcgtg gaagcgctgc
     1261 cgtagtgagt gagtcccgtg ccggagacga ggcagcgcgc cggatccgga tgaaggaggg
     1321 gcgggagcac gcggcgctcc tgcagcaacg ccgtatagga gatggtgggt tttgccgcct
     1381 gtttcgacgc gaacgcgtcc agtggctcgg ccgcggacga tgccgcctgc gccagctcat
     1441 aggtcgactt gaatgaaccg acactgccaa cggtggcgcc gtcgtcagtt acccagccca
     1501 ctcgtggttg accgctttgt tcaaggaatt ggatgagtct caagcgatga atcatgagat
     1561 tcgtatggat atccaacaag cccgggattg ctacggagct atattcatcg gtatatagat
     1621 gcctgggacg ggggaacaca acaagatgtc gacgactacg ccctggttta tccgcgcacg
     1681 gctcaagacg cgtcagctca tgctgctcac ggccatctgc gatgaaggca atattcatcg
     1741 cgccgccgag gtgctgaaca tgtcgcagcc cgcggcctcg aagctcctca aggacctgga
     1801 ggacatgctc ggcgtttcgc tgttcgagcg tcagccgcgt ggcatgcggc ccaattggta
     1861 cggcgaaacc atgatccggc acgcgcgcat cgcgctgtcg tccctgcgcg aagccggcca
     1921 ggaaatcgac gcgctcaagg ctgggttgtc cgggcacgtg agcctcggcg ccatcaccgg
     1981 gcccgcgctc agcctcatcc cgcaggccat catgatcgtc gcgcgcgaga atccgaaact
     2041 gcgcgtggag ctgcacgtgg agtcgagcaa cgtgctcatc gagcaactcg cgcagggcaa
     2101 gatcgacatc atgatcgggc gcctgttcga gcgacaggaa aagactggcc tgcgttatga
     2161 acgcctcgcg gaagaacccg tgtcggcagt agttcggcct ggccacccgc tgctcaatgt
     2221 ggcgggactc agtctcggcg cggtcgaaca caagccgtgg atcgtgccgc cggcgggcag
     2281 tgtgctgcgc catcgcttcg acctcatgtt cagcgaagcg ggttttgcgc cgccgacgca
     2341 gctcatcgag accgcgtcac tgttgttcgt gaccaagatg cttcagcaga ccgacttcat
     2401 tgcggtggtg cccaccgagg tggcgcgcta ctacgcgacc tatggcatgg tcgcgctgct
     2461 gccgatgcag ctttcgtgca cgatggatgc gtttggaatc atcacgcgaa ccgactggtt
     2521 gctgtcgccc gcggccacgg tgttgctgcg cgcgctgaag acgtcggcgg tcagtagtta
     2581 tggctgcacg ctggacatga aaaacctttc ctgagcgtcc ctgatcgctt caaccatgcc
     2641 aaagctcgta tggttctcaa ttagaaaact attactcgcg tatgtctgtg ccttgtacgc
     2701 tgatgcgcga tggacgttcc aaaaaagcgc aagcgacgaa gtcaggactg gttcggccgg
     2761 gaagggaaag acggtttcct gcaccggagc tggatcaaga accagggcta tccggatgac
     2821 ctgttcgatg gccggccggt catcggcatc tgcaacacct ggagcgagct gactccctgc
     2881 aatggtcact tccgcgaact tgcggagttc gtgaagcgcg gcgtctacga ggccggcggt
     2941 tttccgctcg agttccccgt gatgtcgctc ggcgaaacgc agctgcgccc caccgcgatg
     3001 ctgtttcgca atctcgccag catggacgtc gaggaatcca tccgcggcaa ccccctggat
     3061 ggcgtcgtgt tgctcatggg ctgtgacaag accacgccct cgctgctcat gggcgcggca
     3121 agttgcgacc ttcccaccat cggcatctcc ggcggcccga tgttgtccgg gcaatttcgc
     3181 ggcaagcaga ttggctcggg cacgggcgtc tggcagatgt ccgaagacgt gcgcggcggg
     3241 cgcatgtcgc aggaagagtt cacggccgcc gagtcctgca tgcatcggtc gaagggacac
     3301 tgcatgacca tgggcacggc atccaccatg gcgagcatgg tcgaagcgct gggcgtcgcg
     3361 ctgcc
//
LOCUS       DASQJS010000007         9138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_632742, whole genome shotgun sequence.
ACCESSION   DASQJS010000007 DASQJS010000000
VERSION     DASQJS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9138
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632742"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /gene="rpsF"
                     /locus_tag="VEW08_00250"
     CDS             complement(<1..264)
                     /gene="rpsF"
                     /locus_tag="VEW08_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HYJ39185.1"
                     /translation="MRHYEIVFLVHPDQSEQVPAMVERYRGMISAGGGAVHRHEDWGR
                     RQLTFPISKVHKAHYVLLNIECEQATLAELTGAFRFSDAVLRHL"
     gene            complement(454..1236)
                     /gene="rlmB"
                     /locus_tag="VEW08_00255"
     CDS             complement(454..1236)
                     /gene="rlmB"
                     /locus_tag="VEW08_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HYJ39186.1"
                     /translation="MSEKVYGLHAVRSLLTRHAERVTGVTIAEQRADARMTEIQTLAQ
                     KAGKTVKRVPPATFKTLFGDAVHQGVYVEVQPLAAWREEELIAAIVKQLEAAQLRTDK
                     GNAPFVLVLDGVQDPHNLGACLRTADACGALAVIIPKDRAVQMNATVRKVAAGAAETT
                     PIAVVTNLARTLRLLKEAGLWIVGADAEAPKLAHESDLSGPIALVMGAEGSGLRQLTR
                     ETCDLTVRLPQQGAVESLNVSVASGMLLYEALRQRLAVKTGR"
     gene            complement(1233..3329)
                     /gene="rnr"
                     /locus_tag="VEW08_00260"
     CDS             complement(1233..3329)
                     /gene="rnr"
                     /locus_tag="VEW08_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HYJ39187.1"
                     /translation="MKRRNGKSRGSHENPRHAPKPAARRPQGAGAPASSGHGRVAEGI
                     VSANRAGFGFVRVEGQEEAVFLPPPQMAGVMHGDRVRVSVEKGRDGRYSGRLERILEH
                     ATKSFVGTLEVHGRTAFVTASDRRIGMRCMVPPEELSGARHGDWVIAAVTRYAGSGAT
                     PQARIVKRLDPEKPVELACESAIARFDLPREFSPEAVREAESYGNEVDPAEASRRVDL
                     RHLPLVTIDGEDAKDFDDAVYCEPHAKGFRLIVAIADVSYYVRRGTALDASARERGTS
                     VYFPTRVIPMLPFALSNVLCSLQPNVDRLCFASEMIVSRTGALLESRCYTAVMRSVRR
                     LTYDQAFAALFEKRPDALADFGALTEKLLPLVDVYRALLKARHKRGALEFDAAEAEFD
                     FDASGRIQRVYMYQRNEAHKLIEECMILANVAVARELELRGVGTLYRVHGKPEEKKLN
                     LLLETLNALGVAAEFPEDVSPRDFRAITDRLGHDESRPFIESLIVRSMMQAVYQPQNI
                     GHFGLALKHYAHFTSPIRRYPDLVVHRCLRSMLSDSDPYGMRYDGGQLTLAGAELTQL
                     EKRADESDRYVNSWLKCVYLRDRIGQTFEGLITTVVEFGAFVQLTAVGTDGLLHIDSL
                     RDDEYVMEPGGRAWVGRANKRRLGIGARVHVIVTSVNPIEGLIDLSLVTLDAPGKIGM
                     QKSPHRQAKPKTKGRG"
     gene            3432..3516
                     /locus_tag="VEW08_00265"
     tRNA            3432..3516
                     /locus_tag="VEW08_00265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3466..3468,aa:Leu,seq:cag)
     gene            complement(3589..4497)
                     /locus_tag="VEW08_00270"
     CDS             complement(3589..4497)
                     /locus_tag="VEW08_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HYJ39188.1"
                     /translation="MSRRQIDDPRVRVRVRDQVEFLNLVAAAADDELLGFHMSQHCEP
                     RAVGLYYYVLASSGTLTEVFQRAARYSTLVNEGVVQSFIDGRVIGIRTLFTGVSRYRD
                     RHQVEFWTATMTRLLREFTGVPVTPQRVSFAHPANSGVGEMKAYFGCNIEYGAAMDEV
                     IFAKSVGDLPLVNADTYLNRVLVAICEDAYARRATNRGSVRTRVENEIAASLPHGKAR
                     ASIIAIRLGMSQRTLARHLAEEGTSFSAMLTEIRRDLATRYLRDESLSISQIAWLLGF
                     QDLGAFSHAFKRWNGTAPRNAAARAR"
     gene            4387..5157
                     /locus_tag="VEW08_00275"
     CDS             4387..5157
                     /locus_tag="VEW08_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ39189.1"
                     /translation="MEPQQLVVRGCRDQIEELHLVANPYSNTRVVYLTARQTGALQKG
                     CRGNAGSLCMRESEPCDDAHRTWKLRAALHDTGLVTHSDKSLAAGNKTPNVRCTYKAR
                     QFNREPGLAQMELTTEKRARTPGKMGMEKDRKTIAIIDDDNGIRQALKRVLTAAGFSV
                     HMFASAEDFLASMNSCAAGCAVVDLELGKMSGFELACHPDMVAAKIPIIFISGTADET
                     ARASAMALGCVEYLRKPFMPIELLGAILRATQGTPQTS"
     gene            5285..5728
                     /locus_tag="VEW08_00280"
     CDS             5285..5728
                     /locus_tag="VEW08_00280"
                     /inference="COORDINATES: protein motif:HMM:NF022526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2780 domain-containing protein"
                     /protein_id="HYJ39190.1"
                     /translation="MERRALLIGLSLLPLLIAGTAASQTLPGSLQSLSNPLVSKLTSS
                     LGVTQAQAEGGVGSILALAQEKLAKGDFDKVAALIPGASDYLEKARSLGAITGPLLNG
                     AGLNGALGKLGIDAATAAKFVPSVTKFVSKAGGSKLGGMLANALK"
     gene            complement(5749..6402)
                     /locus_tag="VEW08_00285"
     CDS             complement(5749..6402)
                     /locus_tag="VEW08_00285"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYJ39191.1"
                     /translation="MNEPLMRTMSGQITTRIRDKILTGEYSPGSPLLQDSIAAEFGVS
                     KIPVREALVRLRAEGLIDIEAHRGFRVRPLLAAEVDEVFRLRLAIEPAAVNDGTRVAK
                     AKDHIAAREALRSLNEALAAKTMSDIGNLNSAFHLALIVPHQQPVTAEVLSRLHTMSQ
                     RYVRMHLLPVGRVRRATREHNALYEVWAEGDARQARQLTMQHIEETRDELAQFLDHE"
     gene            complement(6399..7328)
                     /locus_tag="VEW08_00290"
     CDS             complement(6399..7328)
                     /locus_tag="VEW08_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HYJ39192.1"
                     /translation="MLRICVIDSHTAGEPTRLIMDGGPDLGDGPLEARLERFRNGFDT
                     YRSAVVNEPRGSDTVVGALLCAPHRADCSVGVIFFNNVGYLGMCGHGTIGLVASLAHA
                     GRLSPGVLRIDTPVGAVAAQLHADGRIDVGNVPSYRKAKGVTVEVPGSGAVRGDVAWG
                     GNWFFLVEAKDLRLALENVEALTDFTWRVRQALNAQGFPEVDHVELFGPSPNTGADCR
                     NFVLCPGKAYDRSPCGTGTSAKLACLAADGALAEGEDWIQESVIGSSFCASFRWLDRS
                     AGTIVPLISGRAHVIAESTLLLDASDPFCWGIR"
     gene            7374..8612
                     /locus_tag="VEW08_00295"
     CDS             7374..8612
                     /locus_tag="VEW08_00295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYJ39193.1"
                     /translation="MAEQETIAVLGAGVIGSAIACALAREGRHVLLIDRAPPGEAGAS
                     FGNAGHIATELVEPLPSPELLFAFWRELFAFGGVLDIPLRRLLKFSPWAVRFARAAFR
                     RAENTRHLAPFVRPAVPALIGMLGEIGRADLIRQHGHYEMWLGGDVARRARHQALAME
                     KLEVPTQTAPAELVETARRAARADTAAGLFFPKCAHVLDPLEVVRAFANAAMARGASF
                     MQREVRSLQARRDGIQIITDAGPIAVRSAIVCAGVWSAPLLAPFGLKAPLEAARGYHV
                     QMPGATPLVDAPILYSDANIVVTPMAGRVRATSFMEFAGTDAPPDPRKPLWLRDTLRG
                     LGYSCVEQPPSWVGSRPVLPDYLPAMGRVAAAPNLLYAFGHQHIGLTLCAVTARTMAD
                     LVAGRTPAVDVSAFDLRRWS"
     gene            8614..>9138
                     /locus_tag="VEW08_00300"
     CDS             8614..>9138
                     /locus_tag="VEW08_00300"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYJ39194.1"
                     /translation="MKKWSGVFPATTTQFASDERLDVAATQKVVDALIDDGVDGVICM
                     GTVGENCSLSAAEKRTVLAAVRETAGGRVPVLSGVAEYTTALAADYARDAEKAGIDGL
                     MVLPAMVYKSNRRETLHHYRTVAQATGLPIMVYNNPVSYGVDITVDMFGELADLPNIV
                     AIKESTEDTRRLTDL"
BASE COUNT         1641 a         3002 c         2866 g         1629 t
ORIGIN      
        1 gaggtgacga agcaccgcat cggagaaacg gaacgcgccg gtgagctcgg ccagggtggc
       61 ctgctcgcac tcgatgttca gcagcacgta gtgcgccttg tgaaccttcg agatggggaa
      121 agtgagctgg cggcggcccc agtcttcgtg acgatgcacc gcgccaccac cggcggagat
      181 cattccgcgg tagcgctcga ccatggccgg gacttgttcg ctctggtccg gatggaccag
      241 gaacacgatt tcgtaatgcc tcatgaatgc tccttgtgga tgttagccac ccgcctgagg
      301 ttggcgatgt ggcaaggaaa accggccaag gtctttgcgg acctaacccg gctcaaaacg
      361 ggcgcgaaga atactcaacg cccaagccaa taacaagggt gttggcgagc cgaaaaccgt
      421 gctccgcggc atgattttca agtacttagg cgactaacgg cctgttttga ccgccaggcg
      481 ctggcgcagc gcctcgtaga gcagcattcc gctcgccacc gacacgttca ggctctccac
      541 ggcgccctgc tggggcagcc ggaccgtgag gtcgcaggtc tcgcgggtca gctgccgaag
      601 tcctgagcct tccgcaccca tcacgagggc aatcggacca ctgaggtctg actcatgagc
      661 gagtttcggc gcctcggcat ccgcacccac gatccacagt cccgcttcct tgagcagacg
      721 aagcgtgcgc gcaagattgg tgacgacggc gatgggagtg gtttcggcgg ctccggccgc
      781 cactttgcgc acggtggcgt tcatctgcac ggcccggtcc ttgggaatga tcacggcaag
      841 cgcaccgcac gcgtccgccg tacgcagaca cgcccccagg ttgtggggat cctgcacacc
      901 gtcgagcacc agcacgaatg gcgcgttgcc cttgtcagtc cggagttggg ccgcctcgag
      961 ttgcttcacg atcgcggcga tcaactcttc ctcacgccag gccgcaagcg gctggacctc
     1021 gacatacact ccctggtgca cggcatcgcc gaacagcgtc ttgaacgtcg cgggtggcac
     1081 gcgcttcacc gtcttgccag ccttctgcgc gagcgtctgg atctccgtca tgcgcgcatc
     1141 ggcgcgttgc tcagcgatcg tgactccggt gacgcgttcg gcgtgacgcg tgagcagcga
     1201 ccgcaccgcg tgcagcccat aaaccttttc gctcatcccc tgcccttggt ctttggcttt
     1261 gcctgccgat gtggcgattt ctgcatcccg atcttgcccg gcgcgtccag cgtcacgagt
     1321 gacaggtcga tgagcccttc gatgggattc acgctggtca cgatgacgtg cactcgtgcg
     1381 ccgattccca ggcgccgctt gttggccctg ccgacccacg cccgcccgcc cggctccatg
     1441 acgtattcgt cgtcacgaag actgtcgata tgcagcagtc cgtccgtgcc cacggccgtg
     1501 agctgcacga atgcaccgaa ttccaccacc gtggtgatga gtccttcgaa ggtctgtcca
     1561 atgcgatcgc gcaggtacac gcacttgagc caggaattca catagcgatc cgattcatcc
     1621 gcgcgcttct cgagctgcgt gagctccgca ccggcgagcg tcagttgccc gccgtcgtat
     1681 cgcatgccgt agggatccga atccgacagc atcgagcgca ggcagcgatg caccaccaga
     1741 tccggatagc ggcgaatggg tgacgtgaaa tgcgcgtagt gcttgagcgc caggccgaag
     1801 tgcccgatgt tttgcggctg gtacaccgcc tgcatcatcg agcgcacgat cagcgattcg
     1861 atgaagggcc gtgactcgtc gtgacccagc cggtcggtta tcgcgcggaa atcgcgcggg
     1921 ctcacgtctt ccgggaactc ggcggcgacg cccagcgcgt tgagcgtttc gagcagaagg
     1981 ttgagcttct tctcttcggg tttgccatgc acgcgataca acgtgccgac accccgcaac
     2041 tcgagctcgc gcgccacggc cacgttggcg aggatcatgc attcttcgat gagcttgtgc
     2101 gcctcgttgc gttgatacat gtacacgcgc tggatgcggc cggaagcatc gaagtcgaac
     2161 tcggcctcgg cggcatcgaa ctcgagcgcg ccgcgcttgt gccgcgcctt gagcaacgcg
     2221 cgatagacgt ccacgagagg cagcaatttt tcagtgagag ccccgaagtc cgccagcgca
     2281 tcgggtcgct tctcgaacag cgcggcgaag gcctggtcgt atgtcagacg gcgcactgat
     2341 cgcatcaccg ccgtgtaaca gcgcgattcc agcagcgcgc ccgtgcgcga gacgatcatc
     2401 tccgacgcga agcacaggcg atcgacgttc ggctgcagcg agcacaacac gttcgacagc
     2461 gcgaacggca gcatcggaat gacgcgcgtc gggaaataca cgctcgtgcc acgctcgcgc
     2521 gcgctggcgt cgagcgccgt tccgcggcgc acgtagtagc tcacgtcggc gatggcgacg
     2581 atgagccgga agcccttggc gtgcggctcg cagtaaaccg cgtcgtcgaa atccttggcg
     2641 tcttcaccgt cgatggtcac cagcggcagg tggcgcagat cgacgcgccg cgacgcttcg
     2701 gcgggatcga cttcgttgcc ataggattcc gcctcgcgga ccgcctcggg agaaaactcg
     2761 cgcggcaggt cgaagcgcgc gatagcggat tcgcaagcga gttcgaccgg cttctccggg
     2821 tcgaggcgtt tgacgatgcg agcctgggga gtggcacccg atcccgcgta acgcgtcact
     2881 gccgcaatca cccagtcgcc gtggcgcgca cccgagagtt cttcgggcgg aaccatgcaa
     2941 cgcatgccga tgcgccggtc ggacgccgtc acgaatgccg tgcggccatg cacttcgagc
     3001 gttccgacga atgatttggt ggcgtgctcg aggattcttt ccagccggcc cgaataccgg
     3061 ccatcccggc ctttctccac ggacacacgt acacggtcgc cgtgcatgac gccggccatc
     3121 tgcggaggcg gcaggaacac cgcttcttcc tggccttcga cgcgcacgaa gccgaagccc
     3181 gcgcggttgg cgctcacgat tccctcggcg acccgtccat gaccggaaga tgcgggggca
     3241 ccggcacctt gaggcctacg tgcggccggt tttggcgcat ggcgcgggtt ttcatgactt
     3301 ccgcgagatt ttccgtttcg acgtttcaaa taattcttcc cggttcatga agggaccatt
     3361 cctttgagga atatgccctt cgattgacag ccaacaaagt ggtgcgtacc tttcgccgct
     3421 cttcgcaccc tgcccgggtg gcggaattgg tagacgcact agtttcaggt actagcgagt
     3481 aacatcgtgg aggttcgagt cctctcccgg gcaccaatca attcaatgaa ttacgcgcat
     3541 ttcgactgcc catggcagcc tgcgccgtgc ctgcgcggat tctacaggtc agcgcgcccg
     3601 ggccgcggca ttccgcggcg ccgtcccgtt ccaacgcttg aacgcgtgtg aaaacgcgcc
     3661 gagatcctgg aatcccagca gccaggcgat ctggctgatc gagaggctct cgtctctcag
     3721 gtagcgagtg gccaggtcac gccggatctc ggtcagcatt gccgaaaagc tcgtaccctc
     3781 ttcggcgaga tgccgtgcca gtgttcgctg gctcatgccg agacgtatcg cgatgatgga
     3841 cgcgcgcgcc ttgccatgcg gaagcgacgc ggcgatctcg ttttccacgc gcgtgcgaac
     3901 ggatccacgg ttggttgccc ggcgcgcata cgcgtcttcg caaatcgcga ccagcacgcg
     3961 gttgagatac gtatccgcat tcaccagggg aagatctccg acgcttttcg cgaaaatgac
     4021 ttcgtccatc gcggcgccat actcgatgtt gcagccgaag taggctttca tttcaccgac
     4081 acctgaattg gccgggtgcg cgaaactcac gcgctgcgga gtgacaggaa ctcccgtgaa
     4141 ttccctgagc aggcgcgtca tcgtcgcggt ccagaattcc acctggtgcc gatcgcggta
     4201 acggcttacc ccggtaaaaa gagtacggat gccgatcacg cgtccgtcga tgaagctctg
     4261 caccacgccc tcgttcacca gggtgctgta acgcgcggcg cgctggaaaa cctcggtcaa
     4321 tgtaccggaa gaagccagca cgtagtaata caggccgact gcgcgcggct cgcagtgctg
     4381 cgacatgtgg aaccccagca gctcgtcgtc cgcggctgcc gcgaccagat tgaggaactc
     4441 cacctggtcg cgaaccctta ctcgaacacg cgggtcgtct atctgacggc gcgacagacc
     4501 ggcgcgttgc agaagggatg tcgcggcaat gccggaagcc tttgcatgcg cgaaagcgag
     4561 ccgtgtgacg acgcccatcg cacgtggaag ctgcgtgctg cgcttcacga tacgggtctt
     4621 gtgactcata gcgacaaatc tctggccgcg ggcaacaaga cccccaatgt ccggtgcacg
     4681 tataaagcgc gccaattcaa ccgggaaccg gggctcgcgc agatggaact aacaaccgaa
     4741 aagcgcgcgc gtacacccgg caagatgggc atggaaaaag accgaaagac gatcgcaatc
     4801 atcgatgacg acaatgggat tcgccaggca ctaaagagag tgctgactgc cgccgggttc
     4861 agcgtccaca tgttcgccag cgccgaagac ttcctcgcca gcatgaactc ctgcgccgca
     4921 gggtgcgccg tggtcgacct ggaactcggc aagatgtccg gttttgaact ggcctgccat
     4981 ccggacatgg ttgcggcgaa gatcccgatc atcttcatca gtggcaccgc cgatgaaacg
     5041 gctcgcgcca gcgcgatggc cctggggtgc gtcgaatatc tgcgcaagcc gttcatgccg
     5101 atcgaactgc tcggcgcgat tctccgcgcc acacagggca ccccgcaaac ttcctgacct
     5161 cgggccatcc tctatctacc cgcccgtcga gccgccggct caatgggacg aagggccaat
     5221 acagaatcgc ggccatcctg cgtaacttcg ccgatccaat cgtcgggaaa cccggagtca
     5281 cgtcatggaa cgccgcgctc tgctgatcgg tttgtccctg ctgccactcc tgatcgccgg
     5341 cactgccgca tctcaaacac tgccgggttc gctgcagtcg ctgtcaaatc ccctggttag
     5401 caaactcacg agctcgctcg gcgtgaccca ggcgcaggcc gaaggcggcg tcgggtcgat
     5461 cctggccctc gcacaggaaa aactcgccaa gggtgacttc gacaaggtcg cggcgctcat
     5521 ccccggcgcc agtgactacc tggaaaaggc caggagcctg ggcgcgatca ccggaccttt
     5581 gctgaacggc gcgggtctca atggcgcgct cggcaagctc ggcatcgacg cggcaaccgc
     5641 cgcgaagttc gttccctcgg taaccaagtt cgtgagcaag gccggcggca gcaaactcgg
     5701 cgggatgctg gccaacgcgc tgaagtgagc ggcgcgcggc cgcgcccgtc attcgtggtc
     5761 gagaaattgc gcgagctcgt cgcgggtttc ttcgatgtgt tgcatggtca gctgccgcgc
     5821 ctggcgcgcg tcaccctccg cccagacttc atagagcgca ttgtgttcgc gcgtcgcccg
     5881 gcgtacgcgc ccgaccggca gaaggtgcat gcgcacgtaa cgctgcgaca tggtgtgcaa
     5941 acggctcagg acttcggcgg tcaccggctg ttgatgcggc acgatgagcg ccaggtgaaa
     6001 ggcgctgttc agattgccta tgtcgctcat cgtcttggcc gccagcgcct cattcaatga
     6061 acgcagcgct tcgcgcgccg cgatgtggtc cttggccttc gcgacccgcg tgccgtcgtt
     6121 gaccgcggca ggctcgatcg ccagccggag gcggaaaacc tcgtccacct cggcggcgag
     6181 caacggccgc acgcgaaagc cgcgatgagc ctcgatgtcg atgagccctt cggcgcgcaa
     6241 acgcaccagc gcttcgcgca ccggaatctt gctgacaccg aactcggcgg cgatcgaatc
     6301 ctggagcagc ggggagccgg gcgaatattc accggtgagg atcttgtcgc ggatgcgcgt
     6361 ggtgatctgc ccgctcatcg tcctcatcaa tggttcgttc atcggattcc ccagcagaat
     6421 ggatcggagg cgtcgagcaa cagcgtgctc tccgcgatga catgggcacg accgctgatg
     6481 agcggcacga tcgttccagc agaccggtcc agccagcgaa aacttgcaca gaagctgctg
     6541 ccgatgacgc tttcctggat ccagtcttcg ccctcggcga gggcgccgtc ggcggccagg
     6601 caggcgagct tggcgctggt acccgtgccg cagggcgagc ggtcgtacgc cttgccaggg
     6661 cacaacacga agttgcggca gtcggcgccg gtgttcggcg acgggccaaa caactccacg
     6721 tgatccactt cgggaaagcc ctgcgcattc aacgcctggc gcacgcgcca cgtgaaatcg
     6781 gtcagcgcct cgacattctc gagcgcaagc cgcagatcct tcgcctcgac caggaagaac
     6841 cagttgccgc cccaggcgac gtcgccgcgt accgcgccgc tacccggaac ttcgaccgtg
     6901 acgccctttg ccttgcgata gctaggcacg ttgccgacgt cgatacggcc gtcagcgtgc
     6961 agctgcgcgg cgaccgcccc caccggcgtg tcgattctga gcacgcccgg cgaaagccgc
     7021 ccggcatgcg ccagcgacgc gaccaggccg atggtgccgt gtccgcacat gccgaggtat
     7081 cccacgttgt tgaagaagat gacgccgacg ctgcagtcgg cgcggtgagg cgcacacagc
     7141 agtgcgccga ccaccgtgtc cgagccgcgc ggctcgttga ccacggccga acggtaggta
     7201 tcgaacccgt tgcgaaagcg ctccaggcga gcttcgagag gtccatctcc caggtccggc
     7261 ccgccgtcca tgatgaggcg cgtgggctcg ccggcggtgt gcgaatcgat gacgcatatc
     7321 cgtagcatgg ctgacctgtt tcctagtgag cgtagatcgt atacggtagc cgcatggctg
     7381 agcaagaaac gatcgcagtc ctcggtgcgg gggtcatcgg cagtgcgatt gcctgcgcgc
     7441 tggcgcgcga aggccggcac gtgctgctca tcgatcgggc accaccgggc gaagccggtg
     7501 ccagtttcgg caatgcgggg cacatcgcca ccgagctggt cgaacccctg ccctcgcctg
     7561 aactgctgtt cgcattctgg cgcgagctgt tcgccttcgg cggcgtgctc gacattcctt
     7621 tgcggcggtt gctgaaattc tctccgtggg cagtgcggtt cgcgcgggcc gctttccgac
     7681 gtgcagagaa cacgcgccac cttgcgccct tcgtgcggcc ggcagtaccg gcgctgatcg
     7741 ggatgctggg cgagatcggg cgtgcggatc tcatccgcca gcacggccac tatgaaatgt
     7801 ggctgggcgg tgatgtcgcg cgacgtgcgc gccaccaggc cctcgccatg gagaagctcg
     7861 aggtgccgac gcagacggcg ccggccgaac tcgtcgagac cgcgcgacgc gccgcgcgcg
     7921 ccgacacggc agcaggcctc ttttttccga agtgtgcgca cgtgctcgac cctctcgaag
     7981 tggtgcgcgc gtttgcaaac gcggccatgg cgcgcggcgc atcgttcatg cagcgcgaag
     8041 tgcgttcgtt gcaagcccgc cgcgacggca tccagatcat caccgacgcc gggccgattg
     8101 ccgtgcgctc ggcaatcgtg tgcgccggcg tgtggtcggc gccgttgctg gcgccgttcg
     8161 gcctgaaggc gccgctcgag gctgcgcgcg gctatcacgt gcagatgccc ggcgcgacgc
     8221 cgctcgtcga tgcccccatc ctctactccg acgccaacat cgtcgtgaca cccatggccg
     8281 gccgcgttcg cgccaccagc ttcatggaat tcgccggaac cgacgcaccg ccggatccgc
     8341 gcaagcctct gtggttgcgc gacacgctgc gcggcctcgg ttattcctgt gttgaacaac
     8401 caccgagctg ggtgggctcg cgtccggttc tgccggacta tctacccgcc atgggccggg
     8461 tggctgcggc gcccaacctg ctctacgcct tcgggcacca gcacatcggt ctcacgttgt
     8521 gcgcggtgac cgcgcggacc atggccgacc tggtcgcggg gcgcacgccc gcggtggacg
     8581 tttccgcatt cgacttacga cgatggagct gacatgaaga aatggtctgg tgtgtttcct
     8641 gcgacgacta cccagttcgc gagcgatgag cgcctcgacg tcgccgccac acagaaggtc
     8701 gtcgacgccc tcatcgatga cggcgtcgac ggcgtcatct gcatggggac ggtgggtgag
     8761 aattgttcgt tgagcgccgc ggagaagcga accgtcctgg ccgccgtcag ggaaacggca
     8821 ggcggacgag tcccggtgtt atccggggtc gccgaataca ccacggcgct ggccgccgac
     8881 tacgcgcgcg acgccgagaa ggctggcatc gatggcctca tggtgctgcc cgccatggtc
     8941 tacaagtcca atcgtcgcga aaccctccat cactaccgca ccgtcgccca ggctacaggc
     9001 ctgcccatca tggtctacaa caacccggtt tcttacggcg tggacatcac ggtcgacatg
     9061 ttcggagaac tcgccgacct gcccaacatc gtcgccatca aggaatccac cgaagacacg
     9121 cgccggctca cggacctg
//
LOCUS       DASQJS010000008         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4087605, whole genome shotgun sequence.
ACCESSION   DASQJS010000008 DASQJS010000000
VERSION     DASQJS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4087605"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VEW08_00305"
     CDS             <1..186
                     /locus_tag="VEW08_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYJ39195.1"
                     /translation="DVPQCGYCQAGQLMSAAALLEKNPAPTDADIDTAMAGNVCRCGT
                     YQRIRAAIHSVAAKRKS"
     gene            186..2450
                     /locus_tag="VEW08_00310"
     CDS             186..2450
                     /locus_tag="VEW08_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYJ39196.1"
                     /translation="MNASMNATVDESLRDLIREMELGTAAAPLRAIKLDRRSFLRLTG
                     LAGGGLVLAFSVGREAEAASATANAGGEFTPNAFLRISRNGSILIYSKGPEIGQGIKT
                     AFPLIIAEELDAKWSDVRVEQAPVNPAVYGRQSAGGSRSIPDSWDQLRRAGAVARHML
                     VGAAAVQWKVPAEECTTRDSAVWHGKRKLGYGALAAKAATLPVPDAATLKLKDRKDYR
                     LLGKWYTGVDNRAVVTGAPLFGIDQTLPGMKIAVYQRCPAVGGKVRSANLAEVKALPG
                     VRDAFVIEGNGKTTEVMPGVAIVADTTWSAMQAQRALRVEWDESQASQDSWSAFAKRA
                     AELAKLPAGETTLQNTGAVDSALASAAKTVSGFYTYPFVAHAPLEPQNCTAWSHDGII
                     EFWAPTQTADRALATVAGALGVPVEQIKINQTRVGGGFGRRLMNDYMCEAGAIAQRFK
                     GPVKLTWSREQDMAHDFFRAGGFHSLTGGVDAAGKLIAWRNHFITFTHDGKAPSSGAG
                     WSGQQFPAQNLPNYQLSQTMLALKTPTGPWRAPASCSVAWVIQSFMHELSAAAGRDHR
                     EFLLEVMGEPRLLEGGLHTGRAADVIRLVTEKAGWGRKLPDGHGMGLAFHFSHAGHIA
                     EVAEVSVDANKRVRLHRVVVASDIGPIVNMSGANNQCEGAVVDGFSTMAGLEITMEKG
                     LIEQANFHQYPILRMPNHPKVKVHFIQSDNPPTGVGEPALPPVAPAICNAIYAAIGHR
                     VRTLPLTKEGFTVA"
     gene            2575..3135
                     /locus_tag="VEW08_00315"
     CDS             2575..3135
                     /locus_tag="VEW08_00315"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HYJ39197.1"
                     /translation="MISPSDLTDLIHRAQNGDSGAVRLLFGAAYPELRRIARSRLRRS
                     SRGTLLDTTALVHESYMRFVKSGRLQIEDRVHFLGYAGQVMRSVIVDFARERHSKRRG
                     GGQHELTLTNGICADDGDAAEQILQIHEAVDGLAKHDPRMVSVVELRYFAGLTEAEIA
                     EVLGIGLRTVRRDWEKARLLLAEALS"
     gene            3132..>3359
                     /locus_tag="VEW08_00320"
     CDS             3132..>3359
                     /locus_tag="VEW08_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39198.1"
                     /translation="MSEQRGVSQLSRLLDEVLDLPPQARTEWIDALAPEFDELKPRLR
                     ALLARCSSVESRDFLSSLPPLGPATTGDDAVV"
BASE COUNT          612 a         1108 c         1101 g          538 t
ORIGIN      
        1 gacgtgccgc aatgcggcta ctgccaggcg ggtcagctga tgtccgcggc cgcgctgctg
       61 gaaaaaaacc cggcgcccac ggatgccgac atcgataccg cgatggcggg caatgtgtgc
      121 cgttgtggca cctatcagcg catacgcgcg gccattcaca gcgtcgccgc caagaggaag
      181 agctgatgaa cgcgtcaatg aatgccaccg tcgacgaatc attgcgcgat ctgatccgcg
      241 agatggagct cggcacggcg gccgcgccct tgcgcgccat caaactggat cgccgcagtt
      301 ttttgcgtct gacggggctc gcgggcggcg gcctggtgct cgcgttctcg gtgggccgcg
      361 aagcggaagc cgcgagcgcc accgccaatg cgggcggcga gttcactccc aacgccttcc
      421 tgcgtatttc acgcaacggc tcgatcctca tctacagcaa gggtcccgag atcggccagg
      481 gcatcaagac tgcgttccca ttgatcatcg ccgaagagct cgacgcgaaa tggtcggacg
      541 tgcgggtcga gcaggcgccg gtgaatcccg cggtgtacgg acggcagagc gcgggcggct
      601 cgcgttcgat tcccgacagc tgggatcaat tgcgtcgcgc cggagccgtg gcgcgccaca
      661 tgctggtggg cgcggccgcc gtgcagtgga aagtgccggc cgaagaatgc accacgcgcg
      721 acagcgccgt gtggcatggc aagcgcaagc tgggttacgg cgcactggcc gcgaaggcgg
      781 cgacacttcc ggtgcctgac gcagccacgc tcaagctcaa ggatcgcaag gactatcgac
      841 tgctgggcaa gtggtacacc ggcgtggaca accgtgccgt ggtcacgggc gcgccactgt
      901 tcggcatcga tcagacgctg ccgggcatga agatcgccgt gtaccagcgc tgccccgccg
      961 tgggcggcaa agtacgcagc gcgaatctcg ccgaggtcaa ggcgctgccg ggcgtgcgcg
     1021 atgctttcgt catcgaaggc aatggcaaga ccacggaagt aatgccgggc gtcgccatcg
     1081 tcgccgatac cacctggagc gccatgcagg cgcaacgcgc gctgcgcgtg gaatgggatg
     1141 aatcacaggc ttcgcaggac agctggagtg cgttcgccaa acgcgccgcc gagcttgcca
     1201 aactacctgc gggcgaaacc acgctacaga acacgggcgc cgtggacagc gcgctggcga
     1261 gtgcagcgaa aaccgtttcg ggtttctaca cctacccctt cgtggcgcat gcaccgctcg
     1321 agccgcagaa ctgcaccgcc tggagccatg acggcatcat cgaattctgg gcgccgacgc
     1381 agacggccga tcgcgcgctt gccaccgtcg ccggcgcact cggtgttccg gtcgagcaga
     1441 tcaagatcaa ccagacccgc gtcggcggcg gattcggccg gcgcctcatg aacgactaca
     1501 tgtgcgaggc cggcgcgatt gcccagcgtt tcaaaggccc cgtgaaactc acgtggtcgc
     1561 gcgagcagga catggcgcac gatttcttcc gcgcgggtgg tttccactcg ctcaccggtg
     1621 gcgtcgacgc cgccggcaaa ctcatcgcct ggcgcaatca tttcatcacg ttcacgcatg
     1681 acggcaaggc gccgtccagc ggcgccggct ggtcgggaca gcaattcccc gcgcagaacc
     1741 tgcctaacta ccagctctcg cagacgatgc tggcgctgaa gacccccacc ggcccgtggc
     1801 gcgcgccggc gtcctgctcg gtggcctggg tcatccagag cttcatgcac gagctgtccg
     1861 cggccgcggg ccgcgaccat cgcgaattcc tgctcgaagt catgggcgag ccgcgactgc
     1921 tcgaaggcgg actgcatacc ggccgcgccg cggacgtcat caggctggtt accgaaaaag
     1981 ccggctgggg gcgcaagctg cccgacgggc acggcatggg cctggccttt cacttcagtc
     2041 acgcgggcca catcgccgaa gtggccgagg tcagcgtcga cgcgaacaag cgcgtgcgcc
     2101 tgcatcgcgt ggtggtcgcg agcgacatcg gtcccatcgt caacatgagc ggcgccaaca
     2161 accagtgtga aggcgccgtg gtcgacggtt tcagcaccat ggccggcctc gagatcacca
     2221 tggagaaagg gctcatcgag caggcaaact tccaccagta tccgatcctg cgcatgccca
     2281 accacccgaa ggttaaggtg cacttcattc agagcgacaa cccacccaca ggcgtgggcg
     2341 agccggcgct gccgccggtg gcgccggcca tctgcaatgc catctacgcg gccattggcc
     2401 accgcgtgcg gacgctgccg ctcacgaagg aagggttcac ggtcgcgtag ctcatcgaac
     2461 gactcatccg gcagcagccg ggtcggcccg cgtggttttg cctgggccaa aaggatcggc
     2521 tagtgtcgaa gccaaaattc gaacacgtag caacgcccgg cctgggacca ccgcatgatt
     2581 tcgccgtcgg atctcaccga tctgattcat cgcgcccaga atggcgactc cggcgccgtt
     2641 cgcctgctat tcggcgcggc ctatcccgag cttcgcagaa tcgcccgttc ccgcctgcga
     2701 cgttcgagcc gtggcacgct gctcgacacc accgcgctgg tgcacgagtc gtacatgcgc
     2761 ttcgtcaaga gcggccggtt gcagatcgag gatcgcgtac attttctcgg ctacgccggc
     2821 caggtcatgc gttcggtcat cgtcgatttc gcgcgcgaac gccatagcaa gcggcgcggc
     2881 ggcggacagc acgaattgac gctaaccaac ggtatttgcg cggacgacgg agatgccgcc
     2941 gagcagattc tgcagatcca cgaagcagtg gatggactag ccaaacacga tccgcgcatg
     3001 gtgagtgtcg tcgagctgcg ctacttcgcc ggcctcaccg aggccgaaat cgcggaagtt
     3061 ctcggcatcg gcttgcggac cgtgcgccgg gactgggaga aagcgcggct gctgctcgcg
     3121 gaggcgctgt catgagcgaa cagcgcggtg tttcgcagct gagccggctt ctcgacgaag
     3181 tgctcgacct gcccccgcag gcgcgcacgg agtggataga cgcgctggct cccgagttcg
     3241 atgagctgaa gccgcgcctg cgggcgttgc tggcgcgctg cagcagcgtg gagtcacgcg
     3301 acttcctttc gtcgttgcca cctctcggcc ccgccacgac cggcgacgat gcggttgtc
//
LOCUS       DASQJS010000009         8335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2205305, whole genome shotgun sequence.
ACCESSION   DASQJS010000009 DASQJS010000000
VERSION     DASQJS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2205305"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VEW08_00325"
     CDS             <1..460
                     /locus_tag="VEW08_00325"
                     /inference="COORDINATES: protein motif:HMM:NF020752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HYJ39199.1"
                     /translation="DMPVYGGELHTSTAATAAFVESMDDDLNTPEAIAVLQGLARDLN
                     TERTAKGESAKAKLLASELRALGGVLGVLGEDPGKFLRSAATVTGTDEGGTPRLPGLS
                     DSEIDTLVAARIAARKEKNFPESDRIRDLLVAAGVALEDKPGGITLWRRG"
     gene            560..636
                     /locus_tag="VEW08_00330"
     tRNA            560..636
                     /locus_tag="VEW08_00330"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:594..596,aa:Pro,seq:tgg)
     gene            684..760
                     /locus_tag="VEW08_00335"
     tRNA            684..760
                     /locus_tag="VEW08_00335"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:718..720,aa:Arg,seq:tct)
     gene            791..866
                     /locus_tag="VEW08_00340"
     tRNA            791..866
                     /locus_tag="VEW08_00340"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:824..826,aa:His,seq:gtg)
     gene            956..1726
                     /locus_tag="VEW08_00345"
     CDS             956..1726
                     /locus_tag="VEW08_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HYJ39200.1"
                     /translation="MAGDSTASEYQPNPKDQQGWGAVLQPFFDEKKLRVVDVARGGRS
                     SRTFITEGHWDRMLTDVHAGDFVIIQFGHNDSGAVNQEPPGSTKPLRARGTIRGIGSE
                     SEEIDNVITGKHETVYSFGWYLRKMIADTRAKGATPILFTLTKTNSWKDGRIPCASDS
                     YRLWTWQIATTEKVAFVDLTRLIADRFQREGVDAVTAQFIDDTVHTNITGAEANAKDA
                     VAGLRAIKKLKFGSKLSRSGRAIRADRGRPKDSVCPKL"
     gene            complement(1723..3723)
                     /locus_tag="VEW08_00350"
     CDS             complement(1723..3723)
                     /locus_tag="VEW08_00350"
                     /inference="COORDINATES: protein motif:HMM:NF045042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase"
                     /protein_id="HYJ39201.1"
                     /translation="MKIRAWMPGALALTLLALPLGAKEKLRYMERLDRGLVAVQQSDG
                     KVFLSWRLLADDPDGVAFNVYRETERPPAAADAGRFSSQSATQTGALRLNPQPLDKGT
                     WLLDSNARLDRETRYFVAAVIDGVEQPRSAPFHIKAGAPALPYHSIALQTPAGYTPND
                     ASVGDLDGDGQYEIVLHQTGAAKDNSQPGVTDAPVFQAYRLDGALLWTINLGHNVREG
                     AHYTQFLVYDFDGDGRAEFVCKTADGTTDGTGKVLGDPDASWLTPESAGEKPGAGFIL
                     SGPEYLTVFDGLTGKALASETYLPRRNPDTDSPTPEQMKETWGDGHGNRIDRFLAGVA
                     YLDGEHPSIVMARGYYTRAVLAAWDFRRDKRGGHLKSRWVFDSDSSEKNKSYRGQGNH
                     NLSVADVDGDGRDEIVYGAAVIDDDGKGLYSTGWGHGDALHVSDLDPANPGLEVFDIQ
                     ERFGPEGMSLRDARSGKALFTIPSVKADESGGDKGEGPGRGVAFNIDPRFPGAESWAA
                     GAGMSGMYDVHGKKFVEGRPAGLSTNFAIWWDGDLLRELLDQNVISKWNWQSQTVEPL
                     LVAHPCASNNGTKATPALSADIWGDWREEVIWRTRDNQELRIYTSTIPTPHRMVTLMQ
                     DPQYRLAVAWQNVAYNQPPHPSFYLDEAAPLPKKPVIRFTRD"
     gene            complement(3720..5210)
                     /locus_tag="VEW08_00355"
     CDS             complement(3720..5210)
                     /locus_tag="VEW08_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ39202.1"
                     /translation="MSTPARRVHFGHYLAYGSNDFLGAGAMSIIGFWILFFYTTFCGL
                     SATQATIIFATARLLDAFFSPVIGYISDRFHNTWLGKKFGRRRFFILLAIPLVPSFAL
                     MWVDGQSFWYYLITYCFFELVYAMEIIPYETLAAEMSPNYQTKAKFAGARIICGQIAN
                     IAAMWLPGVIIAQLGGKESGATFLYLGVIFSVFFVFVALAVYLFTWERPREEIASIAP
                     RDTGGSPFHVITDLFHELWATLRIRAFRLHLGMYIGGYISQDVLNLVLSYIIAFIFLG
                     SVATASSITTWMACAQLVSVGLAIWLTLRIHAAASYRIALGFFSLGILGFATLYFAGV
                     ADIGWFLAVAVIAGLGRGALNFIPWSVYNYMPDVDEIVTGRRREGAFAGVMTFIRKLL
                     QSGVAFAVGPILDASGLIRSVKIQPVHVVNTAVTIMLVGSLGLMIFGFLVSLRFRLNR
                     DTHTVLMDEIERFKNQPGTEPTPENRAVVEDLTGWKYEELWGKGKS"
     gene            complement(5207..6304)
                     /locus_tag="VEW08_00360"
     CDS             complement(5207..6304)
                     /locus_tag="VEW08_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HYJ39203.1"
                     /translation="MVHGIQAAEIDRQIQLLIRNLVEIKDTSGEFLLRLPDGRVIDTK
                     GWNDWEWTHGVGLYGIYKYYQMTRDARCREIMLNWFRDRFAAGTPTKNINTVAPFLTL
                     AYLYEESPDSSWLPYLDEWAEWLMDGLPRTEEMGFQHIVFNSENREQLWDDTLMMSVL
                     PLAKIGRLLGRPQYVEEARRQFMLHIKYLVDRKTGLWFHGWTFDGRHHFANALWARGN
                     CWVTIAIPEFIELLDLQPGDGLREFLLETHLQQIRTLVKHQDASGLWHTLIDDPKSYL
                     EASATAGFAYGILKSVRKGYIGKEYEEAGIRALKAVLANIDKSGELKNVSFGTPVFND
                     LDGYRKIPLTSMPYGQAMAILALGEFKRAFI"
     gene            complement(6339..6650)
                     /locus_tag="VEW08_00365"
     CDS             complement(6339..6650)
                     /locus_tag="VEW08_00365"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HYJ39204.1"
                     /translation="MNYAFRMKLKPGTVDEYKRRHDEIWPELAQLLREAGIYDYSIFL
                     DEEDMHLFAVLKLRDNNKRELLPSHPVMRRWWVYMQDLMYTQPDGRPNEWPLTPMFHL
                     D"
     gene            complement(6647..8215)
                     /locus_tag="VEW08_00370"
     CDS             complement(6647..8215)
                     /locus_tag="VEW08_00370"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HYJ39205.1"
                     /translation="MKKRSFLLHALAGIACLTGAGLAFGAAAPVAITVRADAPGAVIN
                     PTIYGQFAEHLGGLIYGGIWVGEKSPIANTRGYRNDVVAALKELGVPVLRWPGGCFAD
                     EYHWRDGIGPRESRPRKVNTNWGGVVEDNSFGTHEFMDFAELIGADTYVNGNVGTGTP
                     QEMADWLEYMTSDSDSTLAQLRRKNGRDKPWKVNYFAVGNETWGCGGNMRAEYYADLY
                     RHYATFLKTPEGAKPVRIASGSNGSGLPWTEVLMREATKHMDAISVHYYTLPTGKWDV
                     KGAATKFTEDEWWATLVRTWRMEWIVTEHSRIMDKYDAAKRVGLYVDEWGTWYDVEPG
                     TNPGFLYQQNTLRDAVVAAVNLNIFHAHADRVRMTNIAQMINVLQAMILTDKEKMLLT
                     PTYHVFHMYKPFRGATHLPVELEAPQHKSGETSAPTLTVSAARGADGKLHIALVNLDP
                     REAVSGTIRTPGATLKKVSGTILTATAMDAHNTFDKPETVKPAAFKGARVQQGLVHVE
                     VPAKSIVVLSETAR"
BASE COUNT         1494 a         2527 c         2721 g         1593 t
ORIGIN      
        1 cgacatgccg gtgtacggcg gcgaactgca cacctcgacc gccgcaaccg ccgcattcgt
       61 cgagtccatg gacgacgact tgaatacgcc cgaagcgatc gccgtgctcc aggggctggc
      121 gcgggatctc aatactgaac gcacggcgaa gggcgagagc gccaaggcca aattgctggc
      181 gtcagagctg cgcgcgcttg gcggcgtgct cggcgttctc ggcgaagatc cgggcaagtt
      241 cctgcgcagc gcggcgacgg tgacgggcac ggacgagggc gggacgccaa ggcttcccgg
      301 attgagcgac tcggaaatcg acacgttggt ggcggcgcgg atagccgccc gcaaggaaaa
      361 gaattttccc gaatccgacc ggatccgcga tttgctggtc gccgccggcg ttgcactcga
      421 ggacaaaccg ggcgggatca cgctttggcg gcgtggttaa ccaactttcc agtcaaattg
      481 acccgttgcg ggtgcctccg tatcattcgc gcccccgtat gccggggtgg tgatgccccg
      541 acggggcggt gaatcggctc ggggcatggc gcagtctggt agcgcatctg ctttgggagc
      601 agagggtcgc aggttcgaat cctgctgccc cgaccaaccg atgagcgcca ggggtctgtg
      661 aggtagtctt ccgtaccagc tgtgcgcccg tagctcagtt ggatagagca ccggccttct
      721 aagccggtgg tcgcaggttc gactcctgcc gggcgcgcca tttgctggat ttagagagtt
      781 tgtttcaatg gtgggcgtag ctcagctggt agagccccgg gttgtgatct cggctgtcgt
      841 gggttcgagt cccatcgtcc accccatttt tcgcgtcctg cgccttctgg cgctgtgtgc
      901 cggcctcctg ctggcaggcc ctgctgtcgc cgcggacgcg gttccacagt tattcgtggc
      961 cggcgattcg accgcctcgg aataccagcc caatcccaag gatcagcagg gttggggcgc
     1021 cgttctgcag ccctttttcg acgagaagaa gctgcgcgtg gtcgacgtgg cgcgcggcgg
     1081 acgcagcagc cgcaccttta tcaccgaggg tcactgggac cggatgctta ccgacgtgca
     1141 cgccggtgat ttcgtgatca tccagttcgg acacaacgat tcgggcgcgg tgaaccagga
     1201 accgcccggc tcgacgaaac ccctgcgcgc acgcggcacg atacggggga tcggcagcga
     1261 gtccgaagag atcgacaacg tgatcaccgg caaacacgag accgtgtatt cgttcgggtg
     1321 gtacctgcgc aagatgatcg cggacactcg cgccaagggt gcgacgccga tcctgttcac
     1381 gctcaccaag accaacagct ggaaggacgg gcgcatcccg tgcgccagcg acagctatcg
     1441 gttgtggacc tggcaaatcg ccacgaccga aaaagtggcg tttgtcgatc tcacgcgcct
     1501 gatcgcggac cgcttccagc gcgaaggcgt ggatgcggtc acagcgcagt tcatcgacga
     1561 caccgtgcat accaacatca ccggcgccga agccaatgcg aaagacgcgg tggcgggatt
     1621 acgcgcgatc aagaagctga agttcggctc gaagttgtcc aggagtgggc gcgccattcg
     1681 cgccgatcgc ggacggccca aagattcggt ctgtccgaaa ctctaatcgc gcgtgaagcg
     1741 gattacgggc ttcttcggta acggtgccgc ttcatcgaga tagaagctgg gatgcggtgg
     1801 ctggttgtag gccacattct gccaggcgac ggccagccgg tattgcggat cctgcatcag
     1861 cgtgaccatc cgatgcggtg ttggaatggt cgaggtgtag atgcgcaact cctggttgtc
     1921 gcgcgtgcgc cagatcactt cttcacgcca gtcaccccag atgtctgctg acaacgccgg
     1981 agtcgccttc gtgccgttgt tagaagcgca cgggtgcgcg acgagcagcg gttcgacagt
     2041 ctgcgactgc cagttccact tgctgatgac gttctggtcg agaagttcgc gcagcaggtc
     2101 gccatcccac cagatggcaa agttggtgga tagtccggcg ggccggccct ccacgaactt
     2161 cttgccgtgc acgtcataca tgccgctcat gccggcgccc gcggcccagg attcagcgcc
     2221 cggaaaacgc gggtcgatat tgaacgccac gccgcgaccg ggcccttcgc ccttgtcgcc
     2281 gccgctttcg tccgccttca ccgagggaat ggtgaagagt gctttgccgg agcgcgcatc
     2341 gcgcaggctc atgccttcag ggccgaaacg ctcctggatg tcgaacacct cgagtccggg
     2401 attggcagga tcgagatcgc tgacgtgcag ggcgtcgccg tggccccagc ctgtggaata
     2461 caggcccttg ccgtcgtcgt cgatgaccgc cgcgccgtag acgatttcgt cgcggccgtc
     2521 gccatcgacg tcggccaccg agagattgtg gttgccctgg ccgcgatatg acttgttctt
     2581 ctccgaagaa tcgctgtcga acacccaccg gcttttcagg tgtccgccgc gcttgtcccg
     2641 gcggaagtcc caggccgcga gcacggcgcg cgtgtagtag ccgcgcgcca tcacgatgct
     2701 cggatgttcg ccgtcgagat aggcgacgcc ggccaggaac cggtcgatgc ggttgccgtg
     2761 cccatcgccc caggtttcct tcatctgctc gggcgtgggg ctgtcggtgt cgggattgcg
     2821 cctgggtaga taggtctcgc tggccagcgc cttgccggtc aagccatcga acacggtgag
     2881 atattccggt ccgctcagga tgaagcccgc gccaggtttt tcgccggcgg actcgggcgt
     2941 gagccagctg gcatcggggt cgcccagcac cttgcctgtg ccgtcggtgg tgccgtcggc
     3001 ggtcttgcag acgaattccg cgcgcccgtc gccatcgaag tcgtacacca ggaactgcgt
     3061 gtagtgcgcg ccctcgcgaa cgttgtggcc gagattgatc gtccagagca aggcgccatc
     3121 gagcctgtag gcctggaata ccggcgcgtc ggtgacgccg ggttgagagt tgtctttggc
     3181 cgcacccgtc tgatggagga cgatttcgta ctgcccgtca ccgtcgagat cgccaacgga
     3241 cgcgtcgttt ggcgtgtagc cggcaggcgt ctgcagcgcg atcgagtggt agggcagagc
     3301 cggcgcaccg gccttgatgt gaaacggcgc gctgcgcggc tgttcgacgc cgtcgatcac
     3361 tgctgcgacg aagtaccgag tctcccgatc gaggcgggcg ttcgagtcga gcagccaggt
     3421 gcctttatcg agtggctgcg gattcagtcg aagggcgccg gtttgggtcg cggactgcga
     3481 cgagaatcgg cctgcgtcgg cggcggccgg gggccgttcc gtctcgcgat atacattgaa
     3541 cgcgacaccg tccggatcgt ccgcgagcag ccgccagctc aggaacacct tgccatcgct
     3601 ttgctggacc gcgacgagac cacgatcgag cctttccatg tagcgcagtt tttccttcgc
     3661 gccgagtggc aacgccagaa gcgtgagcgc cagcgcgccc ggcatccatg cccgaatttt
     3721 catgacttcc cttttcccca gagttcttcg tatttccagc cggtgaggtc ctcgaccacc
     3781 gcgcggttct cgggagtggg ctcggtgccc ggctgattct tgaaacgctc gatctcgtcc
     3841 atgagcacgg tgtgagtgtc gcgattgagc ctgaagcgca ggctgacgag gaagccgaag
     3901 atcatgagac cgaggctgcc gaccagcatg atggtcaccg cagtgttcac cacgtgcacc
     3961 ggctggatct tgacgctgcg aatgagcccg ctcgcatcga ggatcgggcc cacggcaaat
     4021 gccaccccgg actgcagcag cttgcgtatg aaagtcatta cgcccgcgaa cgcgccttca
     4081 cgcctgcggc ccgtcacgat ttcatcgacg tcgggcatgt agttgtacac gctccacggg
     4141 atgaagttca gcgcgccgcg cccaaggccg gcgatgaccg cgaccgccag gaaccagccg
     4201 atgtcggcca cgcccgcgaa gtagagcgtg gcgaacccga gaattccaag actgaagaag
     4261 ccgagcgcga tgcgataaga agccgcggcg tggatgcgca gcgtgagcca gatagctaga
     4321 cccacggaga ccagctgcgc gcacgccatc cacgtcgtta tcgatgaggc ggtggcgacc
     4381 gatcccagga agatgaacgc gatgatgtac gacagcacga ggttcagtac gtcctggctg
     4441 atgtagccac cgatgtacat gccgaggtgc aggcggaacg cgcggatgcg cagcgtcgcc
     4501 catagttcat ggaacagatc ggtgatcacg tggaacggcg aaccgccggt gtcgcgaggc
     4561 gcaatgctcg cgatctcctc gcgcggccgc tcccaggtga acaggtacac cgcgagcgcc
     4621 acgaacacga agaacaccga aaaaatcacg ccgaggtaga ggaatgtggc gcccgattcc
     4681 ttgccgccga gctgcgcgat gatgacgccg ggcagccaca tcgccgcgat gttggcgatc
     4741 tggccgcaga tgatgcgcgc gcccgcgaac ttcgccttgg tctggtagtt gggggacatc
     4801 tccgcggcca gcgtttcgta cgggatgatt tccatcgcgt acaccagctc gaagaaacag
     4861 tacgtgatca ggtagtacca gaagctctgg ccatccaccc acatgagcgc gaagctcggt
     4921 accagcggga tcgcgagcag gatgaagaag cggcggcggc cgaatttctt ccccaaccac
     4981 gtgttgtgga agcggtcgga gatgtagccg atgaccggcg agaagaacgc gtcgagcagg
     5041 cgcgcggtgg cgaagataat cgtggcctgc gtggccgaga gcccgcaaaa ggtggtgtaa
     5101 aagaacagga tccagaagcc gatgatcgac atcgcaccgg cgcccaggaa gtcgttgctt
     5161 ccataggcga ggtagtgccc gaagtgaacc ctgcgtgccg gtgtgctcat atgaacgcgc
     5221 gcttgaattc gccgagcgcg agtatggcca tggcctggcc gtagggcatc gaggtgagcg
     5281 gaatcttccg gtagccatcc aggtcgttga acactggcgt gccgaaggaa acgttcttga
     5341 gctcgcccga tttgtcgatg ttggcgagca ccgctttcag cgcgcgaatg ccggcttctt
     5401 cgtattcctt gccgatatag cccttgcgca cggacttcag gatgccgtaa gcgaagcccg
     5461 cggtggcgga cgcttcgaga taggacttcg ggtcgtcgat gagcgtgtgc cacaggccgc
     5521 tcgcgtcctg gtgtttcacc agcgtgcgga tctgctgaag gtgggtctcc agcaggaatt
     5581 ctctcaagcc atcgccgggc tgcaggtcga gcagttcgat gaattcgggg atggcgatgg
     5641 tcacccagca attgccgcgc gcccacaacg cgttggcgaa atggtggcgg ccgtcgaagg
     5701 tccagccgtg gaaccacagc ccggtcttgc gatcgaccag gtacttgatg tgcagcatga
     5761 actggcggcg cgcttcctcc acgtattgcg gccggcctag caacctgccg atcttggcga
     5821 gtggcagcac gctcatcatc agcgtgtcgt cccagagctg ctcgcggttc tcggagttga
     5881 acacgatgtg ctgaaagccc atctcctcgg tgcgcggcag gccatccatc agccactccg
     5941 cccactcgtc caggtagggc agccaggaac tgtccgggct ttcctcgtat agataggcga
     6001 gcgtgaggaa cggcgcgacg gtgttgatgt tcttggtggg cgtgcccgcg gcgaagcgat
     6061 cgcggaacca gttgagcatg atctcgcggc aacgcgcgtc gcgcgtcatc tggtaatact
     6121 tgtagatgcc gtacagcccc acgccgtggg tccattccca gtcgttccag cccttggtgt
     6181 cgatgacgcg gccgtcgggc aggcgcagca ggaactcacc cgaggtgtcc ttgatctcca
     6241 cgagattgcg gatcagcagc tggatttggc ggtcgatttc ggcggcttga ataccgtgaa
     6301 ccatgtttgg cacgaaagaa ctcctggaag acgtctgact aatcgagatg aaacatggga
     6361 gtgagcggcc actcgttcgg ccgcccgtcg ggctgcgtgt acatcaggtc ctgcatgtac
     6421 acccaccagc gccgcatgac cgggtggctg ggcaggagct cgcgcttgtt gttgtcgcga
     6481 agcttgagca ctgcaaacag gtgcatgtct tcctcatcga ggaagatcga atagtcgtaa
     6541 atgccggctt cacgcaacag ttgcgcgagc tcgggccaga tctcgtcgtg acggcgcttg
     6601 tattcgtcaa cggtgccggg cttgagcttc atgcggaatg cgtagttcat ctggccgtct
     6661 cgctcagcac gacgatggac ttcgcgggca cctcgacgtg caccagcccc tgctggacac
     6721 gcgcgccttt gaacgccgcg ggttttacgg tctcgggctt gtcgaaggtg ttgtgcgcgt
     6781 ccattgcggt ggccgtgaga atggtgcccg agactttctt caacgtggcg cccggagtcc
     6841 tgatggttcc gctcaccgct tcgcgcggat cgagattgac cagcgcgatg tgcaacttgc
     6901 cgtcggcgcc gcgcgcggcg gagacggtga gagtgggcgc gctggtttcg cccgatttgt
     6961 gttgtggcgc ctcgagttcc accggcaaat gcgttgcgcc gcggaacggc ttgtacatat
     7021 ggaacacgtg gtaggtgggc gtgagcagca tcttttcctt gtccgtgagg atcatggcct
     7081 gcagcacgtt gatcatctgc gcaatgttgg tcatgcgtac ccggtcggcg tgcgcatgga
     7141 agatgttcag gttcacggcc gcgactaccg cgtcgcgaag cgtgttctgc tggtagagga
     7201 aacccggatt ggtgcccggc tcgacgtcgt accaggtgcc ccattcatcc acgtacaagc
     7261 cgacgcgctt cgccgcgtcg tacttgtcca tgatcctgga gtgctcagtg acgatccact
     7321 ccatgcgcca ggtgcgtacg agggtcgccc accattcgtc ttcggtgaac ttcgtggcgg
     7381 cgcctttcac gtcccatttg cccgtaggca gcgtgtagta gtgcacgctg atggcgtcca
     7441 tgtgcttcgt ggcttcgcgc atcagcactt cggtccaggg caggccgctg ccattcgagc
     7501 cgctggcgat gcgcacgggt ttcgcgcctt cgggcgtctt gaggaaagtg gcgtagtgac
     7561 gatagaggtc cgcgtagtac tcggcgcgca tgttgccgcc gcatccccag gtctcgttgc
     7621 cgacagcgaa gtagttgacc ttccacggct tgtcgcggcc attcttgcgg cgaagctggg
     7681 cgagcgtgga gtccgaatcc gaggtcatgt actcgagcca gtccgccatc tcctgcggtg
     7741 tgcccgtgcc gacattgcca ttcacgtacg tgtcggcgcc gatgagttcg gcgaaatcca
     7801 tgaactcgtg cgtgccgaac gagttgtcct ccaccacgcc gccccagttc gtgttcactt
     7861 tccggggacg ggattcgcgc ggaccaatcc cgtctctcca gtgatactcg tcggcgaagc
     7921 agcctcccgg ccagcgcaat accgggacgc cgagttcctt gagcgcggcg acgacgtcgt
     7981 tgcgatagcc gcgcgtattg gcaatcgggg atttctcgcc gacccagata cctccgtaaa
     8041 tcaggccgcc caggtgttcg gcgaactgac cgtagattgt ggggttgatc acggcgcccg
     8101 gcgcgtcggc gcgcacggtg atcgcaacgg gcgctgcggc accgaacgcc aaacccgcac
     8161 cagtcaggca ggcaatgccc gcgagagcgt gcaacaggaa gcttcgtttt ttcatggcaa
     8221 cgacttgagg acaggaagag gagggtgcga gggatcgaaa ctgccggcgt cggctgccag
     8281 atgcttcgca agccctgccg tgagctcggg cggcgcgatc cgcaatccct cgacg
//
LOCUS       DASQJS010000010         3632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_7485, whole genome shotgun sequence.
ACCESSION   DASQJS010000010 DASQJS010000000
VERSION     DASQJS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7485"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VEW08_00375"
     CDS             <1..463
                     /locus_tag="VEW08_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ39206.1"
                     /translation="HHHPGALRTQPLDDPDKLPAFVAQIARNLVIAERRKERRRKTDV
                     DSEAIDAVPDHAQGQEQDAQRESAAVAIRKVLAEMKSIRDRSLLVRYYLRDEDKKDIC
                     RELGLTEPSFNVVLFRARARFLELLEKRGISGEDLFCLVFPAFIATQHWWN"
     gene            466..1086
                     /locus_tag="VEW08_00380"
     CDS             466..1086
                     /locus_tag="VEW08_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39207.1"
                     /translation="MTEQPTAGRYVAGTLTDEEVARFEESMIERPDLAADVNVRRRIK
                     AGLSLLEERRELDTLLAPPVNRPQPLRYAAAAAVLVIAVGLWSVWRMQPATPVQTMFA
                     SADAGMSKVATSFMLVRTRSAQSPAFSVRRNDGLVRLQILIDDPAGAPFTARIGDEEI
                     SITRLIDGFAEVYLDPRKLESRGYTLALKSRSGAEQLFPFALTVTP"
     gene            complement(1160..1729)
                     /locus_tag="VEW08_00385"
     CDS             complement(1160..1729)
                     /locus_tag="VEW08_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYJ39208.1"
                     /translation="MNIEQIRDALPDYARDLKLNLGSVLTTNGAPGLSEKQIWSIALA
                     SAIAARNTSFARSVEMQSKTHLADVEVEGAKAAAAIMGMNNIYYRFLHLVEDGEYQTM
                     PARLRMNVIANHGIDKLDFELISLAVSAVNGCGLCVTSHEKKLRSHDVSREAIQSAVR
                     IAATIHAVAVALEFEQSKPVVAGSAELAA"
     gene            complement(1763..2302)
                     /locus_tag="VEW08_00390"
     CDS             complement(1763..2302)
                     /locus_tag="VEW08_00390"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYJ39209.1"
                     /translation="MLGIGQKFPAYSLTGVVSNDQAKAFQPFDPNSHPGKWRVVFFWP
                     KDFTFVCPTEIAAFGKLDKEFKARDAQLLGVSTDSEYVHHAWRSTKDELKTLPFPMLS
                     DIKRELSTELGILDKDAGVAQRATYIVDPQGVIRFVYVTDLSVGRSPDEVLRVLDALQ
                     TDELCPCNWKQGEDTLKAA"
     gene            2432..3328
                     /locus_tag="VEW08_00395"
     CDS             2432..3328
                     /locus_tag="VEW08_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HYJ39210.1"
                     /translation="MADLKLKDLRYLVAVADTRHFGRAAERSFVSQPTLSAQLKKLEE
                     YLGVQLIERAPKRVSLTVAGEEIVERARRIVEASEEIVELAKGHTDPLSGRLKLALLP
                     TIGPYLLPNVAGKLRKQLPRLELMLYEYQTDVMLEKLHSGEIDVGILALPVPLDGLES
                     YELYREPFTVAMPANHRLAGRSNIKVADLHDETLLLLEDGHCLRDQALEICSGTALHE
                     KQDFRATSLETLRQMVAAGVGITLLPELAGRGAYGAARGVTIKPFAKPVPTRTIGAVW
                     RKSSARRTVILALCKQIEAHAL"
     gene            3347..>3632
                     /locus_tag="VEW08_00400"
     CDS             3347..>3632
                     /locus_tag="VEW08_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39211.1"
                     /translation="MTKAPHRAAAIAAQPLRLRCADGRELAAHWIPAVQRRAVLVVNP
                     ATGFPQTFYFRLASYAAERGYDALVYDYRGMGASAPARLAEETCRMSDWGL"
BASE COUNT          661 a         1154 c         1134 g          683 t
ORIGIN      
        1 tcatcatcat cctggcgcgt tgcgcacaca gccgctggat gatcccgaca aactaccggc
       61 attcgtcgcg cagattgccc gcaacctggt cattgccgaa aggcgcaagg agcgccgccg
      121 caagaccgac gtcgacagcg aagccatcga cgcggtcccc gaccatgccc agggccagga
      181 acaggacgcc cagcgcgaat cggcagccgt ggctatccgt aaggttttgg ctgaaatgaa
      241 gtcaattcgc gatcgatcgc tgttggtgcg ttattacttg agggatgaag acaagaagga
      301 catatgcagg gagctcggcc tgaccgaacc gagcttcaac gtagtcctgt ttcgcgctcg
      361 ggcccgattc ctcgaactgc tcgagaagcg tggcataagc ggcgaagacc tgttttgcct
      421 ggtatttcct gccttcatcg caacgcaaca ttggtggaat tgaaattgac tgaacagcca
      481 accgctggac gctatgtcgc cggaaccctc acggatgaag aggttgcgcg ctttgaggag
      541 tcgatgatcg aacgcccgga cctggcagcc gatgtgaacg tgcggcggcg catcaaggct
      601 ggcttgtcct tgctcgagga acgccgggag ctggatacat tgctcgcgcc gcccgtgaat
      661 cgtccccaac ccctccgtta tgcggccgcg gccgccgtcc tggtgatcgc ggtcgggctt
      721 tggagcgtat ggcgcatgca acccgctact ccggtgcaga ccatgttcgc ttcggcggac
      781 gccggcatgt cgaaggtggc cacaagcttc atgctcgtcc ggacacgctc cgcgcagtcg
      841 cctgctttca gtgtccgccg caatgacggg ctggtgcgtc tgcagattct catcgacgac
      901 ccggccggcg ctccattcac ggcgagaatc ggtgacgagg aaatatccat cacgcgcctc
      961 atcgacggct tcgccgaggt ctacctggat ccacgcaagc tcgaatcgcg tggctacacg
     1021 ctggcgttga aatcgcgttc gggggctgaa cagctcttcc cgttcgctct gaccgtgact
     1081 ccctgaaaaa aaacggcgcc cggttgtccg ggcgccgttc cttgactatc aagggccgtc
     1141 gagggggtga cgccaatgat tacgcagcga gctcggcgga accggcgacg accggcttgc
     1201 tctgctcgaa ttccagggcc acggccacgg cgtgaatcgt cgccgcaatg cgcaccgcgc
     1261 tctggatcgc ttcgcgcgac acatcatgtg aacgcaactt cttctcatgc gaggtcacgc
     1321 acaggccgca gccattcacc gccgacacgg ccagcgaaat cagctcgaag tccagcttgt
     1381 cgatgccgtg attggcgatc acgttcatgc gcagacgcgc gggcatggtc tggtactcgc
     1441 cgtcttccac gagatgcagg aaacggtagt agatgttgtt catgcccatg atggccgcgg
     1501 ccgctttcgc gccctcgacc tccacgtccg cgaggtgagt ttttgactgc atctcgacgc
     1561 tgcgcgcaaa tgaggtgttg cgcgcagcga tcgccgaagc cagcgcgatc gaccagatct
     1621 gcttctcgct gagtcccggc gcgccgttcg tcgtcagcac gctgccgagg ttcaacttga
     1681 gatcgcgcgc atagtcgggc aacgcgtctc taatctgctc aatgttcatc actgactcct
     1741 tattccttat tgcgtggcct tctcaggccg ccttcagcgt gtcttcgccc tgcttccagt
     1801 tgcacgggca cagttcatcg gtctgcagtg cgtcgagcac gcgcagcact tcgtccgggc
     1861 tgcggccgac cgagagatcg gtgacgtaca cgaagcggat cacgccttgc ggatccacga
     1921 tgtacgtggc gcgctgcgca acgccagcgt ccttgtcgag gatgccgagc tcggtcgaca
     1981 gctcgcgctt gatgtccgag agcatcggga acggcagcgt cttcagctcg tccttggtgc
     2041 tgcgccaggc gtggtgcacg tactcgctgt cggtcgaaac gccgagcagc tgcgcgtcgc
     2101 gcgccttgaa ttccttgtcg agcttgccga aggcggcgat ttccgtcggg cacacgaagg
     2161 tgaagtcctt cggccagaag aagaccacgc gccacttgcc tggatgagag ttcgggtcga
     2221 aaggctggaa tgccttggcc tggtcgttgg aaacgacgcc ggtgagcgaa taagcgggaa
     2281 acttctggcc gatacctaac attcaaatac tccttggtga aacactggct gggctctgcg
     2341 ggggcaggcc tggctggaat ggcgtgcatt ctcaggggca aacccggttt cggctaatca
     2401 attgattgta tagactcgat agagagcatc tatggcggac ctcaaactca aggacctgcg
     2461 atacctggtc gccgtggcgg acacccgcca cttcgggcgc gcggccgaac gcagcttcgt
     2521 gagccagccc acgctctcgg cgcagctcaa gaagctcgag gaatacctgg gcgtgcagct
     2581 catcgagcgc gcgccgaaac gcgtgtcgct caccgtggcc ggcgaagaga tcgtcgagcg
     2641 ggcgcgccgc atcgtcgagg cgagcgagga gatcgtcgaa ctggccaagg gccacaccga
     2701 cccgctttcc gggcggctca agctggcgct gctgcccacg atcggcccct atctactgcc
     2761 gaacgtggcg ggcaagctgc gcaagcagct gccgcggctc gagctcatgc tgtacgagta
     2821 tcagaccgac gtgatgctcg agaaactgca ttcgggtgaa atcgatgtcg gaatcctggc
     2881 actcccggtg ccgctcgacg ggctcgagtc ctacgagttg taccgggagc cattcaccgt
     2941 ggccatgccg gcgaaccatc ggctcgccgg gcgctcgaac atcaaggtgg ccgacctgca
     3001 cgacgaaaca ctgctgctcc tcgaagacgg ccattgcctg cgcgaccagg cgctcgagat
     3061 ctgttcgggc accgcgttgc acgagaagca ggatttccgc gccaccagtc tcgagacgct
     3121 gcgccagatg gtggccgcgg gcgtcggcat cacgctgttg cccgagcttg ccggccgcgg
     3181 cgcctacggc gcggcgcgcg gtgtcaccat caagcccttc gccaaacccg tgccgactcg
     3241 caccatcggc gccgtgtggc gcaagtcgag cgcgcggcgc accgtaatcc tggcgctgtg
     3301 caagcagatc gaggcacacg ccctgtgagt cgagcgtgag cgagccatga caaaagcgcc
     3361 tcatcgtgct gcggccatcg cggcgcagcc gttgcgcctg cgttgcgcgg acggtcgtga
     3421 gctcgccgct cactggatac ccgcggtgca acggcgcgcc gtgctggtcg tcaatcccgc
     3481 cacgggattt ccgcagacgt tttatttccg cctcgccagt tacgcggccg agcgcggcta
     3541 cgacgcgctg gtttacgact accgcggcat gggtgcttct gcgcccgcgc gtcttgccga
     3601 agagacttgt cgcatgagcg actggggcct gt
//
LOCUS       DASQJS010000011         9144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3150345, whole genome shotgun sequence.
ACCESSION   DASQJS010000011 DASQJS010000000
VERSION     DASQJS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9144
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3150345"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VEW08_00405"
     CDS             <1..546
                     /locus_tag="VEW08_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HYJ39212.1"
                     /translation="DISYLSGDGRYAILGDMIDLDSDSNLSETRRRGIRARMIETVPE
                     NEMLVFSPKDPKYTITVFTDIDCGYCRRLHSQIADYNKLGIRVRYMFFPRSGPDTESW
                     HKAEAVWCASNRNDALTRAKNGQEVKSPKCPTEIIKRDYELGTRLAVTGTPAIFLASG
                     EMLPGFVPPGELMQYLKTGKM"
     gene            complement(574..897)
                     /gene="fdxA"
                     /locus_tag="VEW08_00410"
     CDS             complement(574..897)
                     /gene="fdxA"
                     /locus_tag="VEW08_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016138692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HYJ39213.1"
                     /translation="MAFVVTESCIKCKYTDCVEVCPVDCFHEGPNFLVIDPEECIDCT
                     LCEPECPVEAIFSEDEVPAGQEEFKKINADLAKKWPVIAEKKAAPPDAKQWEGVAEKK
                     KLLET"
     gene            complement(949..1485)
                     /locus_tag="VEW08_00415"
     CDS             complement(949..1485)
                     /locus_tag="VEW08_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYJ39214.1"
                     /translation="MNFCSNCGTRVVLKVPEGDLLPRHVCENCGTIHYQNPKLVVGSV
                     PSFEGRILLCKRGIEPRQGYWTIPAGFMENDETLEAGAAREAREEALIDVEIGSLLLV
                     ANVTHARQVHIFFRSRMRTPEFGVTHESLEVKLVDEKDIPWSDLAFPSTEYALRRFVE
                     DRAAGVERHHVAEMQRRF"
     gene            complement(1494..1581)
                     /locus_tag="VEW08_00420"
     tRNA            complement(1494..1581)
                     /locus_tag="VEW08_00420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1545..1547),aa:Leu,seq:caa)
     gene            1660..2253
                     /locus_tag="VEW08_00425"
     CDS             1660..2253
                     /locus_tag="VEW08_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYJ39215.1"
                     /translation="MQRLAEIQVPADELSAARAGDAVARSALFERVAPATLGIIRRLV
                     AQKAMAEDLLQDTLIAMFEHLDDYRGEAPFGVWVRSIAVSRCLMAFRSPWHRARVALE
                     SWTEESWSGPEVENRTSDLIDLDRALARLSPVTRAVVWLYDVEGWSHEEIARAFDRTL
                     SFSKSQLARGHARLRNDLLPPVKSGIGIPLRSEFDGH"
     gene            2243..2725
                     /locus_tag="VEW08_00430"
     CDS             2243..2725
                     /locus_tag="VEW08_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39216.1"
                     /translation="MDIEVKDAELVAGLKRLDAAAAQPDVSFGYGALIDRHATRQRRD
                     RRRLAMARGTASALVIALVGVSLWRFEQQPVGSPLDGVEAPAPVVAAPASQPRIVRAD
                     TYFAVAALEDHIASIDDALNDARLSGGAADVARLERARALLVDSYVSVRYAEMVSANF
                     "
     gene            2746..3756
                     /locus_tag="VEW08_00435"
     CDS             2746..3756
                     /locus_tag="VEW08_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HYJ39217.1"
                     /translation="MKQLMLVCLLAASSMAFADTPAADTAKREAEEAKTRAKLEEARA
                     RLDKAAREVAELSGQLGAHARHDVRFIAGGPSRAVLGIQVDPRSDSKGVRVLGVSPGG
                     PAAEAGLLDSDLIIALDGVPTTGGDPDRALIEQMRKVKPEQKVKVRVVRDGKNKDLTV
                     VARPMAFDERMFNVRLPEGAMGAIGGNFDGIQNMPMVKHFRAFVPREFGGMELASITP
                     KLGAYFGVTEGVLVVQAPENDAFKLEDGDVLQAIDGRKPDDGGHALRILRSYKSGEKL
                     NLTVLRQRKPVTLAVTMPERPDMGDNFMFESMMPPTPAMPAMPPSPPIPAVPGAGAST
                     LE"
     gene            3801..4832
                     /gene="queA"
                     /locus_tag="VEW08_00440"
     CDS             3801..4832
                     /gene="queA"
                     /locus_tag="VEW08_00440"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HYJ39218.1"
                     /translation="MRRSDFTYELPPELIAQQPLAERSASRLLTLDGATGALADRQMR
                     ELPQFVAPGDLLVFNDTKVIPARLFALKESGGKVELLLERPLDSTYALVHARASKALR
                     PAMALQSRGGIIRTIEKRGDLWVVELPEAALSFFERYGQMPLPPYIRREPDATDSTRY
                     QSVFARKHGAVAAPTASLHFDEPLLAALGERGVQTAFITLHVGAGTFQPVRTDAVGAH
                     VMHAEFVEVGAAACAAIAAARARGGRVIAVGTTVVRALESAAGAGTLASYVGDSSLFI
                     VPGFRFRVVDAMLTNFHLPESTLLMLVSAFAGRDAVLAAYRHAVAGQYRFFSYGDAMF
                     VTPQPNAHS"
     gene            4829..5959
                     /gene="tgt"
                     /locus_tag="VEW08_00445"
     CDS             4829..5959
                     /gene="tgt"
                     /locus_tag="VEW08_00445"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HYJ39219.1"
                     /translation="MTAEFTLLGTAGLARRGRLKLAHGTVETPAFMPVGTYGTVKAMT
                     PEELQGLGADIVLGNTFHLMLRPGNDVLRAHGGLHGFMNWRRPILTDSGGFQVFSLES
                     LRKITEEGVQFRSPIDGSAVRLTPEDSMDTQLALGSDIAMALDDCTPYPATEQQARDS
                     MERSMRWAERSHAHYYSNNPTGELFAIVQGGMHLPLRLASLETLQKLPWKGFAVGGLA
                     VGEPEEDRLRVLEGLLPNMPADKPRYLMGVGRPQDIIAAVARGIDMFDCVMPTRHARN
                     GHLFTSLGVVNIRNAAHQADTGPLDPACACYTCRNFSRSYLRHLDRCNEILGARLNTI
                     HNLHYYLELMRRLRAGIEAGSLDAVIRDYVREVPDPAQVSVA"
     gene            6037..6369
                     /gene="yajC"
                     /locus_tag="VEW08_00450"
     CDS             6037..6369
                     /gene="yajC"
                     /locus_tag="VEW08_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HYJ39220.1"
                     /translation="MNSLIPDAMAQAPAGAPPGGGLSMFVMMGLFVVIFYFLLIRPQQ
                     KKQKEHQAMLGKLAAGDEVVTTGGIVGRIVEISDNFITLEISEGVRIKVQRFHVTSLV
                     PKGTLKGA"
     gene            6378..8258
                     /gene="secD"
                     /locus_tag="VEW08_00455"
     CDS             6378..8258
                     /gene="secD"
                     /locus_tag="VEW08_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HYJ39221.1"
                     /translation="MLEYARWKYVLVAAVLAVALVFALPNLFGDDYALQIARKDRSAI
                     DSAQLATIESTLKGAGVSYSKVELDGDNVTVRFDTSEAQLKGRDIVKDEKTGLSKEYV
                     NAMMYASRAPRWIQVLGLRAMPLGLDLRGGLYLLYQVDVNGAVESLLNTYDQDFRRVL
                     LAEKISFSDINTLTVDSEIPNGLRVLLPANADLAAVRAALKKAQPDLACRDAVVADGA
                     AVDCVMTEQQVRERRSFAITSNITTLRNRVNELGVSEPLVQQQGVDRIVVQLPGVQNS
                     AEVKDILGKVATLEYRLVDPRPIPANGRAPVGAKIYDRVEGGQVVGKVMLKRDVIVTG
                     NQLTNATATTGQSGPQVDVTLDSAGGDEMLKTTRANLGKPMAVVFIEQRSETTEVDGK
                     PVTRNIKEEKVISVATINGVFGNRFQTTGLTMNEARETALLLRGGALAAPISIANERV
                     IGPQLGKANIEKGVKALVIGMAALFLFMILYYQVFGIVADLVLLANVVVLGALLTMMG
                     VALSLPGIAGIILTVGMAVDANVLIYERIREELRNGVSPQAAIKAGFEKAFSAILDSN
                     VTTFIAGVVLFVFGTGAIKGFAVVLSLGILTSMFTALMGSRALLTLMYGGKRKITKLA
                     IG"
     gene            8284..>9144
                     /gene="secF"
                     /locus_tag="VEW08_00460"
     CDS             8284..>9144
                     /gene="secF"
                     /locus_tag="VEW08_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HYJ39222.1"
                     /translation="MEFFHKKTNFRFMGTRKYWYALSAVLMIVSFASFFTRGLNFAID
                     FTGGISVEAAFPNDANIDKIRAGVEAAGYHEPQVQSFGSTRDVTIRLQSTGESAEVLR
                     PKFEKILTSVDPQAKISDLAVVGPQVGDELRNAAILSLAVTLVLIGVYIILSFHTWRL
                     SLGAIIAVMHDPILVLGVFSVTQTLFDLSVVAAILAVIGYSLNDTVIVFDRIRENFEK
                     NRRQASDVTLDQAINQTLSRTIMTKVVTSIVVVALLLLGGSALRGFSEALLIGIVAGT
                     YSSIYISSAIA"
BASE COUNT         1643 a         2959 c         2874 g         1668 t
ORIGIN      
        1 gacatcagct acctctcggg cgatggccgc tacgccattc tcggcgacat gatcgacctc
       61 gattccgact ccaacctttc cgagacccgg cgccgcggca ttcgcgcgcg catgatcgaa
      121 accgttccgg aaaacgagat gctggtgttc tcgcccaagg atccgaagta caccatcacg
      181 gtgttcacgg acatcgactg cggctactgc cgccgcctgc attcgcagat tgccgattac
      241 aacaagctcg gcattcgcgt tcgttacatg ttcttcccgc gctccggccc cgacaccgag
      301 tcgtggcaca aggccgaagc cgtgtggtgc gcctcgaacc gcaacgatgc gctcacgcgc
      361 gccaaaaatg gccaggaagt caaatcaccg aagtgcccga cggagatcat caagcgcgac
      421 tacgagctcg ggaccaggct cgccgtgaca ggaacgccgg ccatcttcct ggcgagtggc
      481 gaaatgctgc ccggcttcgt gcccccgggc gagctcatgc aatacctgaa gaccgggaag
      541 atgtaggcgc gaaactcccg gaactggtcc gggtcaggtt tcgagcagct tctttttctc
      601 agccacacct tcccactgct tggcatcggg cggggcggcc ttcttctcgg caatcaccgg
      661 ccacttcttg gccaggtcgg cattgatctt cttgaactct tcctgaccgg ccggaacctc
      721 gtcttccgag aagatcgctt ccacggggca ttcaggctcg cacagcgtgc agtcgatgca
      781 ctcctccggg tcgatgacca ggaaattggg accctcgtgg aaacagtcga ccgggcacac
      841 ctcgacacag tcggtgtact tgcacttgat gcacgattcc gtgaccacga acgccatcga
      901 tcaattcctt gggtttgaag cgggattttg gaggggcgcc attctgccct agaaacgcct
      961 ctgcatctcg gccacatggt gccgttcgac accggccgcg cggtcctcga cgaaccggcg
     1021 cagggcatat tcggtgctcg gaaacgccag gtcgctccag ggtatgtcct tctcgtccac
     1081 cagcttcact tccaggcttt cgtgggtgac gccgaattcc ggggtgcgca tgcgcgaccg
     1141 gaagaagatg tgcacctgcc gggcgtgcgt gacattggcg accagcagga ggctgccgat
     1201 ctccacatcg atgagagcct cttcccgggc ttcgcgcgcg gccccggcct caagggtctc
     1261 gtcgttttcc atgaaccccg cggggatggt ccagtacccc tggcgcggtt ctatgccgcg
     1321 cttgcagagc aggatgcgcc cctcgaaaga cggcacgctg cccacgacga gtttcggatt
     1381 ctggtagtgg atggtcccgc agttctcgca gacgtggcgc ggcagcaaat cgccctcagg
     1441 aactttgagg acgactcggg tgccacagtt actgcaaaag ttcatcgatt aagtggtgcc
     1501 gggagaggga atcgaaccca cacgccttat aagggcaacg gattttgagt ccgccgcgtc
     1561 taccagttcc gccatcccgg cgcggggccg gaagtatagc caaggcgatg cgctgatttg
     1621 cgacctttag cggccgcccg gcatctaagc cataagaaaa tgcaaagact cgcggaaatc
     1681 caggtcccgg cagacgagct cagcgccgca cgagcgggcg acgccgtcgc gcgcagcgcg
     1741 ctgttcgagc gcgtggcgcc cgccacgctc ggaatcatcc gtcggctcgt ggcgcaaaag
     1801 gcgatggccg aagacctgtt gcaggacacg ctcatcgcca tgttcgagca cctggacgat
     1861 tatcgcggag aagcgccttt tggagtctgg gtgcgcagta tcgcggtcag ccgatgcctc
     1921 atggctttcc gctcgccctg gcatcgcgcg cgagtcgcgc tcgagtcctg gacggaggaa
     1981 agctggagcg ggccggaggt tgaaaaccgc acctcggatc tcatcgatct cgatcgtgcg
     2041 ctcgcccgcc tgtcgccggt cacgcgcgcg gtcgtctggc tgtacgacgt cgagggctgg
     2101 tctcacgagg agatcgccag ggcattcgac cggacgttga gtttttcgaa gtcgcaattg
     2161 gcgcgcggcc atgcgcggct gcgcaacgac ttgttaccgc cggtgaagtc cggaatcggc
     2221 attccgctca ggagtgagtt cgatggacat tgaagtgaag gatgccgagc tggtcgccgg
     2281 actgaagcgg ctcgatgccg ccgcggcgca gcccgacgtg agcttcggtt atggagccct
     2341 gatcgaccgc catgccacgc ggcagcggcg cgatcggcgc cgcctggcga tggcgcgcgg
     2401 cacggccagc gcgctggtca tcgccctggt gggcgtcagc ctctggcggt tcgaacagca
     2461 gccggtcggc tcgccgctgg atggtgtcga ggcgccggcg cccgtggtcg cagcgcccgc
     2521 cagccagccg cgcatcgtgc gtgccgatac ctatttcgcg gtcgcggcgc tggaggatca
     2581 catcgccagc atcgacgacg cgctcaatga cgcgcgcctg agcggtggag ccgccgacgt
     2641 cgcccgcctg gagcgcgcgc gtgcgttatt ggtcgattcg tatgtgagtg tgcgttacgc
     2701 cgaaatggtg agcgcgaact tctgactggt tgccggagaa ttttcatgaa acagttgatg
     2761 ctggtttgtt tgttagccgc gagctcgatg gcgtttgccg atacgcccgc ggcggatacc
     2821 gcgaagcgcg aggccgaaga agcgaagacg cgcgccaagc tcgaagaggc gcgcgcgcgg
     2881 ctcgacaagg cggcgcgcga agtcgcggaa ttgtcgggcc agctcggtgc gcacgcgcgc
     2941 cacgacgtcc ggttcatcgc gggcgggccg tcgagggcag tgctgggaat ccaggtggat
     3001 ccgcgcagcg acagcaaggg cgtgcgcgtg ctcggcgtga gcccgggcgg tccggcggcc
     3061 gaagcgggcc tgctcgacag cgatctcatc attgcactcg atggcgtgcc caccaccggc
     3121 ggggatcccg atcgcgcact catcgagcag atgcgcaagg tgaagcccga gcagaaagtg
     3181 aaggttcgcg tagtgcgcga tggcaagaac aaggatctga ccgtcgttgc tcggcctatg
     3241 gcattcgatg aacgcatgtt caacgtgcgt ttgcccgagg gggccatggg cgccatcggc
     3301 ggcaactttg acggcatcca gaacatgccg atggtgaagc acttccgcgc gttcgttccc
     3361 cgtgagttcg gcggcatgga gctcgcgagc atcacgccga agctcggtgc ctatttcggt
     3421 gtgaccgagg gcgtgctcgt ggtccaggcg ccggagaatg acgcattcaa actcgaagac
     3481 ggtgatgtgc tgcaagccat cgatggccgc aagcccgatg atggcggtca cgcgctgcgc
     3541 atcctgcgtt cgtacaagtc gggcgagaaa ctcaacctca ccgtgctgcg ccagcgcaaa
     3601 cccgtgacgc tcgcggtgac catgccggaa cgcccggata tgggtgacaa cttcatgttc
     3661 gaatccatga tgccgcccac accagccatg ccggcgatgc cgccgtcgcc gccgattccg
     3721 gccgtgccgg gagcgggggc cagcacgctc gaatgacgtg acaaggatga tggcggccat
     3781 ggggcggtaa actcgccgcc gtgcgccgct ccgacttcac ttacgaactt ccacccgaac
     3841 tcattgccca gcaaccgctc gccgagagat cggcgagccg cttgctgacg ctcgacggcg
     3901 ccaccggcgc gctcgccgac cggcagatgc gcgagctgcc gcagttcgtg gctcccggcg
     3961 acctgctggt cttcaatgac acgaaagtga ttccggcgcg cctgttcgcg ctcaaggagt
     4021 ccggcggcaa agtggagctg ctgctcgaac ggcccctcga ttcaacgtat gcactggtgc
     4081 acgcacgcgc cagcaaggcg ttgcggccgg ccatggcatt gcaaagccgc ggcggaatca
     4141 tcaggaccat cgagaagcgc ggtgacctct gggtggtcga actaccggaa gccgcgctct
     4201 cgtttttcga acgttacggc cagatgccgc tgccgcccta catccggcgc gaacccgacg
     4261 ccaccgacag cactcgctac cagagtgtat ttgcgcgcaa acatggcgcg gttgccgcac
     4321 ccaccgccag cctgcacttc gacgagccct tgctcgcggc actcggcgag cgcggcgtgc
     4381 agaccgcgtt catcacgctg cacgtgggcg caggaacctt tcagcccgtg cgtaccgacg
     4441 cggtgggcgc gcacgtcatg cacgcggaat tcgtcgaagt gggcgcggcg gcctgcgcgg
     4501 ccatcgccgc agcgcgtgcc cgtggcgggc gcgtcattgc cgtgggcacg accgtggtgc
     4561 gtgcgctgga aagcgcagcg ggcgcaggga cgcttgcgtc atacgtgggt gattcatcgc
     4621 tgttcatcgt gcccggattc cgcttccggg tcgtggatgc gatgttgacc aacttccatc
     4681 tgcccgaatc cacgctgctc atgctggtga gcgcatttgc cggccgcgac gccgtgctgg
     4741 cggcataccg gcacgcggtc gcggggcaat atcgcttttt cagttatggt gacgccatgt
     4801 tcgtgacccc ccagcccaac gcccattcat gacagccgag ttcactttgt taggtaccgc
     4861 gggcctagcg cgccgcggcc gcctgaagct cgcgcacgga accgtcgaaa cgccagcatt
     4921 catgcccgtg ggcacctacg gcacggtcaa ggcgatgacg cccgaagagc tccaggggct
     4981 cggcgccgac atcgtgctgg gcaacacgtt tcacctgatg ctgcgtccgg gcaatgacgt
     5041 cctgcgcgcg cacggcgggc tgcatggatt catgaactgg cgacggccga tcctcaccga
     5101 ctccggcggc ttccaggtgt tcagtctcga atcgctgcgc aagatcaccg aggagggcgt
     5161 gcagttccgc tcgcccatcg acggcagcgc cgtgcggctg acacccgagg attcgatgga
     5221 tacgcaactc gcgctgggtt cggacatcgc gatggcgctc gatgactgca cgccgtatcc
     5281 ggccaccgaa caacaggcgc gcgactcgat ggagcgttcg atgcgctggg ctgagcgcag
     5341 ccacgctcac tactacagca ataatccaac cggcgagttg tttgccatcg tgcagggcgg
     5401 catgcatctg ccgctgcgac tggcctcgct cgagacactg cagaagcttc cgtggaaggg
     5461 gttcgcggta ggtggactcg cggtgggcga acccgaagaa gaccgtttgc gcgtgttaga
     5521 gggtttgttg ccgaacatgc ccgccgacaa accgcgctat ctcatgggcg tggggcggcc
     5581 tcaggacatc atcgcggcag tggcccgcgg catcgacatg ttcgactgcg tcatgcccac
     5641 gcggcacgca cgtaatgggc acctgttcac gtccctgggt gtggtgaata tccgcaacgc
     5701 cgcccaccag gcggacaccg ggccgcttga cccggcctgc gcctgctata cctgccggaa
     5761 tttctcgcgc tcctatctgc gtcacctgga ccgttgcaac gaaatcctgg gggccaggct
     5821 caatacgatt cacaacctgc actactacct cgagctcatg cgccggctgc gtgccggcat
     5881 cgaagccggc tcgctggatg cggtgattcg cgactatgtc cgtgaagtgc ccgatcccgc
     5941 gcaagtctct gtggcataaa tttttttttc ggccaaaata cgcggccgcg ccgcctcccg
     6001 gggcgccgtt ttttccgtca cgtgtgagat aacaccatga attcattgat ccccgacgcc
     6061 atggctcagg cccctgccgg cgcccctccg ggcggcggac tgtccatgtt cgtgatgatg
     6121 ggcctgttcg tcgtcatctt ttacttcctg ctcatccgtc cgcagcagaa gaaacagaaa
     6181 gaacaccagg cgatgctggg caagctcgcc gccggcgacg aagtggtcac cactggcggc
     6241 atcgtcggcc gcatcgtgga aatcagcgac aacttcatca cgcttgaaat ctccgaaggc
     6301 gtgcgcatca aggtgcagcg tttccacgtc acctcgctgg tgccgaaggg cacgctcaag
     6361 ggagcctgag gtcttaaatg ctcgagtacg cgcgctggaa atatgtgctc gttgccgcgg
     6421 ttctggcggt ggcgttggtg ttcgccctgc ctaacttgtt tggcgacgac tacgccctgc
     6481 agatcgcgcg caaagatcgc agcgccatcg acagcgcgca gctcgcgacc atcgagtcca
     6541 ccctcaaggg cgccggcgtc agctacagca aagtggagct cgacggcgac aacgtcacgg
     6601 tgcgattcga caccagtgaa gcgcagctca agggccgcga catcgtcaag gacgagaaga
     6661 ccggcctcag caaggaatac gtgaacgcga tgatgtacgc gtcgcgtgcc ccgcgctgga
     6721 tccaggtgct gggattgcgc gccatgccgc tcggtctcga ccttcgcggc ggtctttatc
     6781 tgctgtacca ggtcgacgtc aacggcgcgg tggagagcct gctcaacacc tacgaccagg
     6841 actttcgccg cgtgctcctg gccgagaaga tttccttctc tgacatcaat acgctaaccg
     6901 tcgattcgga aattcccaat ggcttgcgcg tgctgctgcc cgcgaacgcc gatctcgccg
     6961 cggtgcgcgc ggctctcaag aaggcgcaac ccgatctcgc ctgtcgtgac gccgtcgtcg
     7021 ccgatggcgc cgcggtcgac tgcgtgatga ccgagcagca ggtccgcgag cgccgcagtt
     7081 tcgccatcac ctcgaacatc accacgctgc gcaatcgcgt caacgagctc ggcgtgtccg
     7141 agcccctcgt gcagcagcag ggcgtcgatc gcatcgtggt gcagttgccc ggcgtgcaga
     7201 actccgccga ggtgaaagac atccttggca aggtggcgac gctggaatat cgcctggtcg
     7261 atccgcggcc catccccgcg aacggtcgcg cgccggtggg cgccaagatc tacgaccgcg
     7321 tcgaaggcgg ccaggtggtt ggcaaggtca tgctcaaacg cgacgtcatc gtcaccggca
     7381 accagctcac caacgcgacc gccaccaccg ggcagagcgg tccgcaggtg gacgtgacgc
     7441 tcgatagcgc tggcggcgat gaaatgctga agacgacgcg cgccaatctc ggcaagccca
     7501 tggccgtggt gttcatcgaa cagcgcagcg agaccaccga agtcgatggc aagccggtta
     7561 cgcgcaacat caaggaagag aaagtcatca gcgtcgcgac catcaacggc gtgttcggca
     7621 accgcttcca gaccactggc ctcaccatga acgaggcgcg cgagaccgcg ctgctgctgc
     7681 gcggtggcgc gctggcggca cccatctcca tcgccaatga acgcgtcatc ggtccgcagc
     7741 tcggcaaggc gaacatcgag aagggcgtca aggcgctggt catcggcatg gccgcgctgt
     7801 tcctgttcat gatcctctac taccaggtgt tcggcatcgt cgccgacctg gtgctgctcg
     7861 ccaacgtcgt ggtgctggga gcgctgctca ccatgatggg ggtggcgctg tcgctgcccg
     7921 gcatcgccgg catcatcctc accgtcggca tggcggtgga cgcgaacgtg ctgatctacg
     7981 aacgcatccg cgaggaattg cgcaacggcg tttcgccgca ggcggcgatc aaggcgggct
     8041 tcgagaaagc cttctcggcc atcctcgatt ccaacgtcac caccttcatc gcaggcgtcg
     8101 tgctgttcgt gttcggtacc ggcgccatca agggttttgc cgtcgtgctg tcgctcggca
     8161 tcctcacgtc catgttcacc gcgctcatgg gctcgcgtgc cctgctcaca ttgatgtacg
     8221 gcggcaaacg caagatcacc aaactggcga tcggctgacc gaatcaatac aaggacctgc
     8281 acagtggaat tttttcacaa gaagaccaac ttccggttca tgggcacgcg caagtattgg
     8341 tacgcgctct cggccgtgct gatgatcgtg agctttgcgt cgtttttcac gcgcggactg
     8401 aactttgcca tcgatttcac cggtggcatc agcgtcgagg cggcctttcc gaacgacgcc
     8461 aacatcgaca agatccgcgc cggcgtcgag gcggccggat accacgagcc gcaggtgcag
     8521 tccttcggct cgacgcgcga cgtcaccatc cgtctgcagt ccaccggcga atccgccgag
     8581 gtactgcgcc cgaagttcga aaagatcctc acgagcgtcg atccacaggc gaagatctcc
     8641 gacctcgccg tggtgggtcc gcaggtgggt gacgaactgc gcaacgcagc gatcctctcg
     8701 ctggcagtga cgctggtcct gatcggcgtc tacatcatcc tgagtttcca tacctggcgc
     8761 ctgtcgctcg gcgccatcat cgccgtgatg cacgatccca tcctggtgct cggcgtgttc
     8821 tcggtcacgc agacgctgtt cgacctgtcg gtggtcgcag ccatcctcgc ggtcatcggc
     8881 tactcgctga acgataccgt catcgtgttc gaccgcatcc gcgaaaactt cgagaagaat
     8941 cgccgccagg cctccgacgt cacgctcgac caggccatca accagacact gtcgcgcacc
     9001 atcatgacca aggtggtcac ctccatcgtc gtggtcgcgt tgctgttgct gggcggttcg
     9061 gcgctgcgcg gcttctccga ggcgctgctc atcggcatcg ttgccggcac gtattcgtcc
     9121 atctacattt cgagcgccat cgcg
//
LOCUS       DASQJS010000012        17869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_637895, whole genome shotgun sequence.
ACCESSION   DASQJS010000012 DASQJS010000000
VERSION     DASQJS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17869
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637895"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VEW08_00465"
     CDS             complement(<1..1112)
                     /locus_tag="VEW08_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYJ39223.1"
                     /translation="MRSRYGALAIYSTLLACVAVGTLTLAFLQGVDFPAWWVVLLGIG
                     SCLFVWQFGLRAPRLGLISMERLPQIGLLLVFEPVVAASICAAASLTWPLVSRRYSQG
                     SFKVAGLRAIHNGSMTALMLMLAGHAYLASGGRHPLTGLVATDLVPLIAMSLTAQIVN
                     VGLMMLFFRFDGRDVRSIVTPSYALSDLIFVPAGVLAAVLYNIGGVEVFWLFAGLMLL
                     FVLSFNSIGMQRDPVPADRGPLTRLFEARLALQGARRVDELAERILTETRSLFRFDEL
                     YLVLVDRERRQLDFRVHERQRIRLPVRLEPLETGLFGWLIARAEPLLVEDWRHASPEL
                     LQRAEAEKVSGSLLAMPLVNDGIVLGLLCVQHSEAG"
     gene            complement(1206..2126)
                     /locus_tag="VEW08_00470"
     CDS             complement(1206..2126)
                     /locus_tag="VEW08_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HYJ39224.1"
                     /translation="MRIRLLLLLLVAPFANAEPVIFDTDIGTDVDDAYALVALIRRPE
                     LELLGVTTVSSDAVARARLAAKLLSVAGGKWSSVPVYAGISTATQYMKQVEWADGFTS
                     PSLHDDGGIEFMRRQINARPGEITLIAVGELTNIAALLESEPGIGKKIRAISLMGGAV
                     HRGYAPGSKPEPEWNIKSNAAAARTVFTSGVPLLVAPLDSTADLKLTPDMRVQLFSRG
                     TPLHDALAALDQVWRHTNHWKGNDPTLFDVLAVELVAPRTPYKLTALSIEVAADGLTK
                     PVATGKPNAQVALVVDVPAFMKQFVETLSR"
     gene            complement(2152..2895)
                     /locus_tag="VEW08_00475"
     CDS             complement(2152..2895)
                     /locus_tag="VEW08_00475"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HYJ39225.1"
                     /translation="MVSLREMQRAFAAALRDSSAACPVLPAGNLSIYRKNSAISFRSA
                     LESSFPVVRRRVGDDYFRQLSAQYRARFPSRSGDLHWIGRDFPAFLLEHLHGSEYAWL
                     ADLARLEWSREKASIARVEPSVAADVLGRFAPTQLEHLVFTLQPSLSLVASDFPIFTI
                     WAANQVDNAPPVDQSLSGECGMVRARIDLLEVRPLEPRLFSYLSALAAGAPLPDAIVS
                     AGLDQAGLVSALGFVFTEGLVCAVRETGT"
     gene            complement(2879..3736)
                     /locus_tag="VEW08_00480"
     CDS             complement(2879..3736)
                     /locus_tag="VEW08_00480"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HYJ39226.1"
                     /translation="MPRLELRGAPGPIPACAGIGFRAVHQHALATERPAVGWIEAHTE
                     NYFHESGPAGRALERARANYPLSLHGVGLGLGSADGVDREHLAKVKGAIARFEPALVS
                     EHACWGHAGGEFFNDLLPLPYTDEAVELLARQVGEAQDFLGARLLIENVSAYVTFEHS
                     HLHEWDFLAAVAATSGCGLLLDVNNVYVSSQNLGLDARGFIDGLPPDRVAEIHLAGHA
                     RNGAVLIDDHGSQVCDAVWELYRYAVNRFGALPTLIEWDTNIPALATLVAESRRADLI
                     LGEARGLAA"
     gene            complement(3743..4003)
                     /locus_tag="VEW08_00485"
     CDS             complement(3743..4003)
                     /locus_tag="VEW08_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HYJ39227.1"
                     /translation="MNLKTIAIASAVGSLLALGSATASAEDAPAKEKCLGVAEAGKND
                     CANSKHSCAGQAKADKGADEWKYVPKGECEKMGGKVVEKKAM"
     gene            4396..7488
                     /locus_tag="VEW08_00490"
     CDS             4396..7488
                     /locus_tag="VEW08_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ39228.1"
                     /translation="MSSHRNRGASRALALLCGLSATLATSLVIAQDKPADDKAMDEVV
                     VTGSRIARPNLESPVPVTTVSSEELFQTGTTSVGDLLNDLPALRSTFSQSNSSRFLGT
                     TGLNLLDLRGLGTQRTLVLVNGRRHVGADILSNAVSPDVNTFPTDLIESIDVVTGGNS
                     AVYGSDAIAGVVNFILKKDFTGLQFRGQAGTSDEGDAGNYYGSILGGTNFMDDRGNIA
                     FNFEFAKQDAWFASDRDAYDNNAGFVVVDSDPSGSDGVPDRRFYDDIRFASVANAGSF
                     LIANNAMAPTLTPCGVDPLGARYSCSYIFDGANSLVPQTGQRIGLAPTGNFQGGNGMN
                     NREEDLLAIYPQLDRYSFNLFGHLQISEAFTPFIEAKYVRTDSIRYGSPAFFQGGTTG
                     AAQETVRFDNPFLSNQARATLQQARVNAGFTTPYADTDRLALRKNLIEFGPRREDATR
                     ETTRVVLGVEGQMTENWNYEVAYNYGQFDEDTIVRGNMNLQRFGLAMDAALNPTGQVV
                     CRSQIAPAPSDLYDPGIPLAVATLAGDIAACAPLNPFGQGNISQAARDYVLQDTTSVG
                     QIKQQVISATITGDSSALFSLPAGAIGIALGFEQRTEENFFEADELVSSGITFYNALP
                     LFDPPKFKVSEIFAELRVPLLKELPFAEELTLNLAGRFADYDGNTGEVFAYNAGLDYS
                     PISSLRFRAGLARAVRAPSLVDLYSEQSQNFATINDPCAARFIGAGASTRAANCAAAG
                     IPASYDYTYPSTPEILSGGNPDLLEETSDSLTVGFVWQPEFAAGLSFSADYFDIEIDD
                     VITAPTAQQIMNACYDASDIDNQFCGLFQRWGAAGSPTSGPADAYALMNGTLQQTLLN
                     YASSIAEGIDFEFGYSHSAGDAGKIKTRLLYTLVLDRTDFLDPSNPSDGDQAMLELGD
                     PKDSFNLNVDYEVGAVSIGYQLRYIGKQVLFNYEDTYSFQGEPPQNADYAETRYYPST
                     MFQDIRLGYDINEGLNAYLGCENFTDELPPLGLTGATEGGGIYDPRGRYFYAGVKFEF
                     "
     gene            complement(7634..8350)
                     /locus_tag="VEW08_00495"
     CDS             complement(7634..8350)
                     /locus_tag="VEW08_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYJ39229.1"
                     /translation="MQMLGSLLFNVFLYLWMLVFAFTYCVVCPFLSFEGRFRLAGVMT
                     HVVFFAARVLCGIHYTVEGRENLPEGNHVIFMKHSSTWETFGIMILFPPLVWVMKREI
                     LWIPFVGWGCVLASCIAIDRKAGASAVNQVLVQGKDRMARGAWILVFPEGTRMAAGET
                     RRYGSSGTLLAAQENRKIVPVAHNAGYFWPRRGVLKKRGTIRVIIGPPIDPVGRDVRE
                     VNSEVQAWIESHVVPPDSAE"
     gene            complement(8375..10465)
                     /gene="glyS"
                     /locus_tag="VEW08_00500"
     CDS             complement(8375..10465)
                     /gene="glyS"
                     /locus_tag="VEW08_00500"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HYJ39230.1"
                     /translation="MSSALDFLVELGTEELPPLALPELERAFAAGIRAGLAEAGLPHG
                     ELRSFATPRRLAVWVRDLAVMQPAQAIKLKGPPVSAAFDKAGKPTTAATRFAEKCGAD
                     VAALTRITEGKGEFLYFAGSKPGLATGGLLPAIVQRSLDQLPIPKRMRWGSSSAEFVR
                     PVHWLVMLFGADVIPARILDTDAGRATRGHRFMAPQELALASPSDYETVLREKGKVIP
                     DFGERRLRIGTQVAAAAKSLSGEALLSDSLLDEVTALVEWPNAITGSFEARFLELPRE
                     VLISTLQQHQRYFPVLGADAKLLPHFITVANIESREPDKVRAGNERVVRPRLSDAAFF
                     WSQDRKQPLAARLPGLDAVTFQAKLGSIGDKVRRVLTLSGEIALLIDADRAKAERAAS
                     LAKCDLLSSMVGEFPELQGIMGRYYAAADGEPADIAVAIDEHYLPRGAGGALPGTGAG
                     VAVALADKLDTLAGIFAIGQKPSGTKDPFGLRRAAIGALRILIEKRIDLDLRALVIRA
                     CALQPVQNANASGEVWDYMVERLRAYFLDSGSAGGVAGVSTEMFDAVRASSPVSPLDF
                     GARLAALAKFLGIPEAGSLTAANKRIANILKKADLGASGTVDVAALLEPAEKELHEAL
                     AGVVPEVERALAKRDYSAALTRLATLRPAVDGFFDGVMVNAEDPALRRNRLALLAQVR
                     HQFTRIADLSCLPG"
     gene            complement(10462..11361)
                     /gene="glyQ"
                     /locus_tag="VEW08_00505"
     CDS             complement(10462..11361)
                     /gene="glyQ"
                     /locus_tag="VEW08_00505"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HYJ39231.1"
                     /translation="MPSTTPRTFQDLIFALQKYWADQGCVILQPYDMEMGAGTFHTAT
                     FLRSIGPEPWSAAYVQPSRRPTDGRYGDNPFRLQHYYQFQVVIKPSPLPILDLYLGSL
                     RALGLDPLVHDIRFVEDNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLDCKPVT
                     GEITYGLERLAMYLQGVESIFDIVWTAGPRGVVTYGDVYHQNEVEQSAYNFEHADTAV
                     LLRHFDDHEAACGKLLEAKLALPAYEQVMKASHTFNLLDARKAISVTERQRYILRVRT
                     LARAVAQAYYDSREALGFPMVKA"
     gene            complement(11450..12142)
                     /locus_tag="VEW08_00510"
     CDS             complement(11450..12142)
                     /locus_tag="VEW08_00510"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HYJ39232.1"
                     /translation="MVERSYTARPVKFAPCHRIVASRLPTIHLFERVADPADWDALYE
                     LESRTNPRIRTELGELSLVPVTDRVSGPNASIVMAPFTHLSPAGTRFTNGHYGAYYAA
                     ESVDTAIAETRFHRESFLRATSQPPIELEMRCYLADIACDLHDLRARRMEMPDIYDPA
                     SYTASQKLGRELRDAGSNGIAFDSVRRNGGECVAIFRPRLVQNVRQSVHLRYAWDGNA
                     ISEVYEIRIVNL"
     gene            complement(12173..12586)
                     /locus_tag="VEW08_00515"
     CDS             complement(12173..12586)
                     /locus_tag="VEW08_00515"
                     /inference="COORDINATES: protein motif:HMM:NF042861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HYJ39233.1"
                     /translation="MILNVASTRHDPKSSTSDVTSLGGAGLRAFMNIAEAWGLTVAEQ
                     LKLLGIASRSTFFKWRRERNPRLPRDTLERLSYLLGIYKSLQILLPDPQLADQWVRKP
                     NTAPTFGGRSALDRMLSGQVADLYVVRQYLDTERG"
     gene            12853..14262
                     /gene="glnA"
                     /locus_tag="VEW08_00520"
     CDS             12853..14262
                     /gene="glnA"
                     /locus_tag="VEW08_00520"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HYJ39234.1"
                     /translation="MKPADVIKLIKDKEVKYADLRFTDTRGKEQHVTIPANLVDEDLF
                     RDGKMFDGSSISGWKGINESDMILALDPTTAVVDPFFEQTTVNIRCDIIEPATMQGYE
                     RDPRSLGKRAEAYLKSTGIADGALFGPENEFFIFDSVRWSSGMNETSYSIDSGQGAWN
                     SNTIYPDGNKGHRPTIKGGYFPVPPVDAFQDIRSAMCDALAEMGLKVEVHHHEVATGG
                     QCEIGVGPGGLVKKADEVQILKYAIHNVAHSYGKTATFMPKPLVGDNGSGMHVHQSLQ
                     KAGVALFAGDKYGGLSDTALYYIGGIIKHAKAINAFTNPGTNSYKRLVPGFEAPVMLA
                     YSARNRSASIRIPWVSNPKARRIEVRFPDSAANPYFAFAAMMMAGLDGIQNKIHPGEP
                     ADKDLYDLEPEEAKHIPTVCHSLDMALEHLDKDREFLTRGGVFTNDVIDAYIGLKTQE
                     VTRFRMCTHPVELDMYYSC"
     gene            14482..15033
                     /locus_tag="VEW08_00525"
     CDS             14482..15033
                     /locus_tag="VEW08_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HYJ39235.1"
                     /translation="MSLCAASAALATTYVRVEKDGSKTYSDRPLPGGQPIDLQPAQTY
                     STPPPGVSAESQQPREEQLLRELEDFRYQSCTVTPENDTTFQNPESVNIAVSINPSLR
                     PGDVVTMTVDGQPAGPPNSLTYTMSPVYRGTHTVGLSVANPKGKVVCNSTSVFHVQQP
                     GMNSPARQAPPRPRPPPPRPTPH"
     gene            15061..16155
                     /gene="glnL"
                     /locus_tag="VEW08_00530"
     CDS             15061..16155
                     /gene="glnL"
                     /locus_tag="VEW08_00530"
                     /inference="COORDINATES: protein motif:HMM:NF008293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HYJ39236.1"
                     /translation="MAVEEHFLPSAIAHFEPADLIGALTTGIVMLDSDLCVVYANVAA
                     QDLLAVSVNQARGKPFLSLVLEADSLGVLMRRAVDSGETLSERELAVQTGPLGREHRV
                     VDVTMTPLDSEFDRKYLLLEIADATQRQRITRENELISRLDSSRQMVRQLAHEIKNPL
                     GGLRGAAQLLERELHEHLREYTGIIISEADRLTALVDSMIGPARAPQKSLINVHELCE
                     HVYRLLRSEARGGVLVERDYDPSLPNAEVDANQIIQALLNIGRNALLAIGERGRIIIR
                     TRVAANVNIGATRHKLAASLSVEDNGPGVPPDIADTIFFPLVTGRANGTGLGLAVSQD
                     LVTRHGGLIEFESKPGRTVFTILLPLPENA"
     gene            16173..17606
                     /gene="ntrC"
                     /locus_tag="VEW08_00535"
     CDS             16173..17606
                     /gene="ntrC"
                     /locus_tag="VEW08_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HYJ39237.1"
                     /translation="MHTTSPPSASQPALRVWLVEDDASIRWVLERALRASGMVPRAFD
                     SAEPALVALRTDAPDVLLTDIRMPGSSGIDLLREIRVSHPTLPVIVMTAHSDLPSAVS
                     AYEGGAFEYLPKPFDIDQAVELVRRAGQTARKGDEVPAQTPEIPELLGKAPAMQQVFR
                     AIGRLSRSSVAVLITGESGTGKELVARALHEHSPRQAKPFVALNTSAIPAELLESELF
                     GHEKGSFTGADTQRRGRFEQANLGTLFLDEIGDMSTPLQTRLLRVLAEGEFYRVGGQT
                     PIKVDVRVIAATHQNLEERVERGIFREDLYHRLNVIRIELPPLRSRTEDVPDLLAHYL
                     RQAANELGTETKSLAPITLERLQAYRWPGNVRELVNLCRRLTALAPGSEIHLEDLPSE
                     MHIVTPAAQTDWAAALTLWAKAQPHDGTVAWLDQALPEFERVLIRVALNRTHGHRQEA
                     ARVLGWGRNTLTRKLKELGMSDDESLP"
BASE COUNT         3450 a         5718 c         5574 g         3127 t
ORIGIN      
        1 atgccggctt ccgagtgctg cacgcataac aggcccaaca cgatgccatc gttgaccagc
       61 ggcatggcga gcagcgaacc ggaaaccttt tccgcctcgg cacgttgcaa caactcgggc
      121 gacgcgtgcc gccagtcttc gacgagcaat ggctccgcgc gtgcaatcag ccagccaaac
      181 aagccggtct cgagtggctc caggcgcacg ggcagacgga tccgctggcg ttcatgcaca
      241 cggaaatcca gctggcgccg ctcgcgatcg acgagcacga gatacaactc atcaaaacgg
      301 aacagcgagc gcgtctcggt caaaatgcgc tccgcaagct cgtcgacacg gcgcgcgccc
      361 tgcagcgcaa ggcgagcttc gaacaggcga gtgagaggcc cgcgatccgc cgggaccgga
      421 tcgcgctgca tgccgatgct gttgaagctc aatacgaaca gcagcatcag gccggcgaac
      481 aaccagaaca cttccacgcc gccgatgttg tagagcacgg ccgcaagcac gcctgcggga
      541 acgaagatca ggtccgagag cgcgtagcta ggcgtgacga tgcttcgaac atcgcggccg
      601 tcgaaacgga agaacagcat catcaggccg acgttcacga tctgtgccgt cagcgacatc
      661 gcgatcaagg gcaccaggtc cgtcgcgacg agccccgtga gcgggtgccg gccgccgctg
      721 gcgagatagg catgacccgc gagcatcagc atcagtgccg tcatggagcc gttgtggatc
      781 gcgcgcagcc cggccacttt gaacgacccc tggctgtagc ggcggctgac gagcggccac
      841 gtcaatgatg cggcggcaca aatactggcg gcgaccacag gctcgaagac cagcagcaag
      901 ccgatctgcg gaaggcgctc catcgagatt aggccgaggc gcggggcacg caggccgaac
      961 tgccagacga acaggcagga accaatgccg agcagcacca cccaccaggc cggaaaatcg
     1021 acaccctgca gaaacgccag cgtgagcgtg ccgacggcaa cgcaagcgag cagcgtcgaa
     1081 tagattgcca gtgcgccgta acgagagcgc atgcgcggac gacttcggtg gcgcggcgta
     1141 accgcgactc ggccactgtg gcacgaggcg gtaaatgggg gaagaggctg ggccccggcg
     1201 ctcgatcagc gcgagagcgt ctcgacgaac tgcttcatga aagccggcac atccaccacg
     1261 agagcgacct gcgcgttggg tttgccggtg gcgactggct tggtgagccc gtcggccgcc
     1321 acctcgatgc tgagtgccgt cagcttataa ggagtgcgtg gcgcgaccag ctccaccgcc
     1381 agcacatcga acagcgtcgg gtcgtttcct ttccagtggt tggtgtggcg ccagacctga
     1441 tcgagcgcgg cgagcgcgtc atggagcggt gtgccgcgtg aaaacaactg cacccgcata
     1501 tccggcgtca gcttcaggtc tgccgtggaa tcgagcggtg ccaccagcaa tggcacgccg
     1561 gaagtgaaca cggtgcgcgc ggcggccgca ttcgatttga tgttccattc aggctccggc
     1621 ttcgaccctg gcgcatagcc acgatggacg gcgccaccca tgagtgagat ggcgcggatc
     1681 tttttgccga tgccgggctc ggactcgagc agcgcggcga tgttggtcag ctcacccacg
     1741 gcgatcagcg tgatctcacc gggacgcgca ttgatctgcc ggcgcatgaa ctcgatgcca
     1801 ccgtcgtcat gcagactcgg cgacgtgaac ccatcggccc actcgacctg cttcatgtat
     1861 tgggtcgcgg tcgagattcc ggcatacacg ggaacactcg accacttgcc accggcgacg
     1921 ctcaacagtt tggccgcgag ccgcgcgcgc gccaccgcat cgctcgagac cgtggtgact
     1981 ccgagcagct cgagttcggg ccgccggatc aatgccacga gcgcgtaggc gtcatcgaca
     2041 tcggtaccaa tgtcggtgtc gaaaatcacc ggctccgcat tcgcgaatgg cgccaccagg
     2101 agcaacaaca gaagtctgat tcgcatgcga ccgagtatag gttcgcccgg atcaggttcc
     2161 cgtttctcgc accgcgcaca ccaggccctc ggtgaagaca aacccaagag cgctcacgag
     2221 accggcctga tcgagaccgg cgctgacgat ggcatcaggc agcggggcgc ccgcggcgag
     2281 cgcagataga taagagaaga ggcggggctc caggggtcgc acttccagta gatcgatgcg
     2341 cgcgcggacc ataccgcact ccccagacag agattgatcc actggcggtg cattatcgac
     2401 ctgattcgcc gcccagatcg tgaaaatcgg gaagtccgag gcgaccagtg acaaagacgg
     2461 ttgcagcgta aaaacgaggt gctcgagctg ggtgggagca aagcgcccga gtacgtcggc
     2521 cgctaccgac ggttcaaccc tggcgatcga agccttctcg cgggaccact cgagccgggc
     2581 caggtcggcg agccacgcgt attcgctgcc gtgaagatgt tcgagcagaa atgcggggaa
     2641 gtcgcggccg atccagtgga ggtcgccact gcgtgaagga aatcgtgcgc ggtactgggc
     2701 actcagttgg cggaagtagt cgtcaccgac gcgccggcgc accaccggga aactgctctc
     2761 gagcgcggag cggaatgaaa tcgcggagtt cttgcgatag atggatagat tgcccgccgg
     2821 cagcacagga cacgcggcgg aagagtcgcg cagcgcggcc gcgaacgcac gctgcatttc
     2881 acgcagcgag accacgcgcc tctcccagga tcagatccgc gcgccggctc tcggcgacca
     2941 gggtcgcgag cgccgggatg ttggtgtccc attcgatgag agtgggcagc gcgccgaatc
     3001 gattcacggc gtaccgatac agttcccaga ccgcatcaca aacctgcgac ccgtgatcgt
     3061 cgatgagcac ggcgccattg cgcgcatggc cagcaaggtg aatctcggcg acgcggtccg
     3121 gcggaagtcc gtcgatgaag cctcgcgcat cgagcccgag gttctgcgaa ctcacgtaca
     3181 cattgttgac gtcgaggagc aggccgcatc ccgaggtcgc agcgacggcg gcgagaaaat
     3241 cccactcgtg caggtgggaa tgttcgaacg tcacatacgc cgacacattt tcgatgagca
     3301 acctggcgcc gaggaaatcc tgggcttccc cgacctggcg ggcaagcagt tccacggctt
     3361 cgtcggtgta gggcagtggc agcagatcgt tgaaaaactc gccgcccgcg tggccccagc
     3421 aggcgtgctc ggacaccagc gcgggttcga aacgtgcgat ggcgcctttc actttcgcga
     3481 ggtgctcgcg atcgacaccg tctgcggaac ccaggccaag tccgacgccg tgaaggctga
     3541 gcgggtagtt agcgcgtgcg cgctcgagtg cgcggccggc cgggccgctt tcgtggaaat
     3601 agttttcggt gtgcgcttct atccagccaa cggccggacg ctcggtggcg agcgcgtgct
     3661 gatgaacagc ccgaaagccg attccggcac acgccggaat cggcccgggc gcacctcgta
     3721 gttcgaggcg cggcatgagt cgttacatcg ccttcttttc gacgaccttg ccgcccatct
     3781 tttcgcactc gcctttcggt acgtacttcc actcgtcggc acccttgtcg gccttggcct
     3841 gtcctgcgca ggaatgcttg gagttggcgc agtcgttctt gccggcttcg gcaacgccca
     3901 ggcacttttc cttggccggg gcatcttcgg cggatgcggt ggcactgccg agcgccagca
     3961 ggctgccgac ggctgaagca atggcgatgg tcttcagatt catgtgactt ctccggagtt
     4021 gttgtctttc gaatagcggg atcaaccgga acaggggacc cggggatcga tcgctttatt
     4081 tcgtatcagc gccgatagag gcatgttcgt ccacccgccc ggcgccggca accgtttgct
     4141 tttctgcgac aggtcggatt cggggttatt cgctaaaaat tagacccgtt tttgtgcctt
     4201 gccagctcga aaccatgcaa atagtcggtt tcccgtccca taagcggttt ccttttttcc
     4261 actgattgtt atgctccgga catggatgtg tgcgtgtaac aacacgcctg catcaaggga
     4321 tcttaaaaaa atagctgttc cagcagcgaa gcgatcgctg cggcccgaac cgccaactct
     4381 cttgcaggag ggaccatgtc ttctcatcgc aatcgcggcg caagccgcgc actggcattg
     4441 ctttgcggac tatccgcaac gctcgccacg tctcttgtta ttgcccagga caaacccgcc
     4501 gacgacaaag cgatggacga agtcgttgtc acgggttccc gtattgcccg tcccaatctc
     4561 gaatcgccgg tgcccgtgac cacggtttcg agtgaggaac tgtttcagac aggcactacc
     4621 tcggtcggcg accttctgaa cgacttgcct gccctgcgca gcacgttcag ccagtcgaac
     4681 tccagccgtt tcctgggaac cactggtctc aacctgctcg atctgcgtgg cctcggcacg
     4741 cagcgcaccc tggttctggt caatggccgt cgccatgtcg gtgccgacat tctcagcaat
     4801 gccgtttcgc ccgacgtcaa cacgtttccc actgacctca tcgagagcat cgatgtcgtg
     4861 accggcggca actccgctgt gtacggctcc gacgccatcg cgggcgtggt caacttcatt
     4921 ctcaagaagg acttcacggg attgcagttc cgtggccagg ctggcaccag cgacgaaggt
     4981 gatgccggta actactacgg cagcattctc ggcggcacta acttcatgga tgaccgcggc
     5041 aacatcgcgt tcaactttga gtttgccaag caggatgcgt ggttcgcctc tgatcgcgac
     5101 gcctacgaca acaacgcagg tttcgtggtg gtcgactccg acccatctgg cagcgacggt
     5161 gttccggacc gacggttcta cgatgacatc cgcttcgctt cggtagccaa cgccggttca
     5221 ttcctgatcg cgaacaacgc catggcgccg acgctcactc catgcggagt ggacccgctc
     5281 ggtgcgcgct acagctgttc gtacatcttt gacggtgcca attcgctcgt gccgcagacc
     5341 ggccagcgca tcggccttgc ccccacgggc aattttcagg gtggcaacgg catgaacaat
     5401 cgcgaggaag accttctcgc gatttatccg caactcgatc gctacagctt caacctgttc
     5461 ggtcatctgc agatttccga agcgttcacg ccgttcatcg aagccaaata cgtgcgtacg
     5521 gactccatcc gctacggcag ccccgcgttc ttccagggcg gcacgaccgg cgctgcgcaa
     5581 gaaacggttc gtttcgacaa cccgtttctg tcgaatcagg cgcgtgcgac tctgcaacag
     5641 gcgcgtgtca acgccggatt cacgaccccg tatgcggata cggaccgact cgcgctgcgc
     5701 aagaacctca tcgagttcgg cccacgtcgt gaagacgcga cccgcgagac gactcgagtc
     5761 gtcctcggtg tcgagggcca gatgacggag aactggaatt acgaagttgc gtacaactac
     5821 ggccagttcg acgaagacac gatcgttcgc ggcaacatga acctgcagcg cttcgggctt
     5881 gccatggacg cggcgctcaa cccgaccggt caggtcgttt gccgctcgca gatcgctccc
     5941 gccccgagcg acctgtacga ccccggcatt ccgctcgccg ttgcgactct cgccggcgac
     6001 atcgcagcct gcgcgccgct gaatccgttc ggccagggca acatctcgca ggcggctcgc
     6061 gattacgtgt tgcaggacac gacttcggtc ggccagatca agcagcaggt catcagcgca
     6121 acgattacgg gtgattccag cgcgctgttc tcgctgccgg ccggcgcgat cggcatcgcg
     6181 ctcggattcg agcaacgcac cgaagagaac ttcttcgagg ccgatgagct ggtctcgagc
     6241 ggcatcacgt tctataacgc gctgccgttg ttcgacccgc cgaagttcaa ggtcagcgaa
     6301 atcttcgcgg aattgcgcgt gccgctgctc aaggagctgc cgttcgccga agagctcacg
     6361 ctgaatctcg caggtcgctt tgccgactat gacggcaata cgggtgaagt gttcgcgtac
     6421 aacgcgggtc tggactactc gccgatttcg agcctccggt tccgtgccgg cctggcacgt
     6481 gcggtgcgtg cgccgagcct cgtggacctg tactcggagc agagccagaa cttcgcgacg
     6541 atcaacgatc cttgcgcggc ccggttcatc ggtgctggtg cttccacgcg tgcggcgaac
     6601 tgcgccgcgg caggtatccc ggcgtcgtac gactacacgt acccgtcgac tccggaaatc
     6661 ctgagcggcg gcaacccgga tctcctggag gagacttcgg attcgttgac ggttggattc
     6721 gtgtggcagc ccgagttcgc ggcaggtctg tctttctcgg ccgactactt cgacatcgag
     6781 atcgacgatg tgatcacggc gccgacggcg cagcagatca tgaacgcatg ctacgacgcg
     6841 tctgacatcg acaaccagtt ctgcggactg ttccagcgct ggggtgcggc gggcagcccg
     6901 acgtcgggtc ctgccgatgc ctatgcgctc atgaacggca cgctgcagca gacgttgctg
     6961 aactacgcca gctcgatcgc cgagggcatc gacttcgagt tcggatacag ccattcggct
     7021 ggcgacgcgg gcaaaatcaa gacacgcctg ctctacacgc tggtgctcga ccgtacggac
     7081 ttcctggatc cgtccaatcc gtccgatggg gatcaggcga tgctcgaact cggtgatccg
     7141 aaggactcgt tcaacctgaa cgtggactac gaagtgggag cggtgtcgat cggctaccag
     7201 ctgcggtaca tcggcaagca ggtgctcttc aactatgaag acacctactc gttccagggc
     7261 gagccgccgc agaacgcgga ctacgccgag acccgctact acccgagcac gatgttccag
     7321 gacatccgcc tcggctacga catcaatgaa ggcttgaacg cctacctcgg ctgcgaaaac
     7381 ttcaccgacg agctgccgcc gttgggcctg acgggcgcaa cggaaggtgg cggtatctac
     7441 gacccacgcg gtcgttactt ctacgccgga gtcaagttcg agttctaagt agcagtccgg
     7501 gccggcagac cgctgccggc ccggcctaac taaatcgagt taccggacct tcgcggcata
     7561 cgaggcctgc cgcctcgctg cttgggtcaa gaggccgggt ttacacccgg cctttttttt
     7621 gcgcggctcg gcctcactcg gcgctgtcgg gcggaacgac gtggctctcg atccacgcct
     7681 gcacttcact gttcacttcg cgcacatcgc gcccgaccgg gtcgatgggt gggccgatga
     7741 tcacgcgaat cgtcccgcgc ttcttgagca cgccgcgccg cggccagaag tagccagcgt
     7801 tgtgcgcgac cggaacgatc ttgcggttct cctgcgcggc taacaaagtt ccgctggaac
     7861 cgtagcggcg ggtttcgccg gccgccatgc gcgtgccctc gggaaatacc aggatccacg
     7921 cgccacgcgc catccggtcc tttccctgca ccagtacctg gtttaccgcc gacgcaccgg
     7981 ccttgcgatc gatcgcgatg cagctcgcca gaacgcagcc ccagccgacg aacggaatcc
     8041 acaggatctc gcgcttcatg acccagacga gaggcgggaa caggatcatg attccgaaag
     8101 tttcccacgt cgacgaatgc ttcatgaaga tcacgtgatt gccttccggc agattctcgc
     8161 ggccttcgac ggtgtagtgg atgccgcaca agactcgcgc cgcgaagaac accacgtgag
     8221 tcatgacgcc ggccaggcgg aagcggccct cgaatgagag aaacgggcac accacgcaat
     8281 acgtgaacgc gaacaccagc atccacaggt acagaaagac gttgaagagg agcgagccga
     8341 gcatctgcat gattgcgata ccccgttgtg cgggtcagcc cggcaggcag gacaggtcgg
     8401 caatgcgcgt gaactgatgg cgcacctggg cgagtaatgc caggcgattg cgccgcaacg
     8461 cggggtcctc cgcattgacc atcacaccgt cgaagaagcc gtcgaccgcg gggcgcaaag
     8521 ttgcgaggcg ggtcagcgcc gcactgtagt cgcgtttcgc cagcgctcgt tcgacctccg
     8581 gcacgacgcc ggctagcgct tcgtgcagct ccttctcggc aggctcgagc aatgcggcga
     8641 cgtcgaccgt gccggatgcc ccgagatcgg ccttcttgag gatattcgcg atgcgtttgt
     8701 tcgccgcggt gagacttccc gcctcgggaa tcccgaggaa tttcgccagg gcggcgagcc
     8761 tcgcgccgaa atcgagcggc gagaccggcg agctcgcacg cacggcatcg aacatctccg
     8821 tggagacacc ggcgacaccg ccggcgcttc cgctatcgag aaagtacgca cgcaaccgtt
     8881 cgaccatgta atcccacact tcgccggagg cgttcgcgtt ctgcacgggc tgcagagcgc
     8941 aggcgcggat gacgagcgcg cgaaggtcga ggtcaatgcg cttctcgatg aggattctca
     9001 gcgcgccgat ggcggcacgg cggaggccga aaggatcctt ggtgccgctc ggcttctggc
     9061 cgatcgcaaa gattccggca agcgtgtcca gcttgtccgc cagcgctacg gcgacaccgg
     9121 cgcccgtgcc cggcagggcg cctccggcgc cgcgcggtag atagtgctcg tcgatcgcca
     9181 cggcgatatc ggcgggctcg ccatcggccg cggcgtagta ccgacccatg attccctgca
     9241 attcgggaaa ttctcccacc atcgaggaca gcaggtcaca cttggccagg ctggcggccc
     9301 gctcggcctt cgcgcggtcg gcgtcgatga gcaaagcgat ctcgccggag agagtcagca
     9361 cgcggcggac cttgtcgccg atggaaccga gcttggcctg gaaagtgacc gcgtcgagac
     9421 ccggcaagcg cgccgcgagg ggctgcttgc gatcctggct ccagaagaat gctgcgtcgg
     9481 acaaccgcgg ccttaccacg cgttcgttgc cggcccgcac cttgtcgggc tcacggctct
     9541 cgatattggc cacggtgatg aagtgcggca gtagtttggc gtcggctccg agcacgggaa
     9601 agtaacgctg gtgctgctgc agcgtggaga tgagcacttc gcgtggcagc tcgaggaatc
     9661 gtgcttcgaa cgagccggtg atggcgttgg gccattccac gagggcggtg acttcatcga
     9721 gcagcgaatc gctgagcaac gcttcgccgg acagggattt cgccgcggcg gcaacctgcg
     9781 tgccgatacg caggcggcgc tcgccgaagt ccggaattac tttgcctttc tcacgcagca
     9841 cggtttcgta gtcggaagga ctcgcgagag ccagttcctg cggcgccatg aagcgatggc
     9901 cgcgcgtcgc gcgccctgca tcggtgtcga gaatgcgcgc gggaatcacg tccgcgccga
     9961 acagcatcac cagccagtgc acggggcgaa cgaattccgc gctcgatgag ccccagcgca
    10021 tgcgtttcgg aatgggaagc tggtcgagcg agcgttgcac gatggccggt aacaggccgc
    10081 ccgtcgcgag gcccggcttc gagcccgcga aatagaggaa ctcgcctttg ccttcggtga
    10141 tgcgcgtgag tgcagcgaca tcggcaccgc acttctcggc aaacctggtc gcggcagtgg
    10201 tgggcttgcc ggccttgtcg aatgcggcac tgaccggcgg ccccttgagc ttgatggcct
    10261 gggccggctg catgacggcg agatcgcgga cccagacggc gagccgccgc ggcgtcgcga
    10321 acgaacgaag ctcgccatgt ggcaatccag cttcggcgag gccagcgcga atgccggcgg
    10381 caaaagcgcg ttcgagttcc ggcagcgcga gcgggggcag ctcttcggtg cccagctcca
    10441 cgagaaagtc gagcgcggaa ctcatgcttt caccatggga aaaccgagcg cctcgcggct
    10501 gtcgtaataa gcctgggcca ccgcacgcgc aagtgtgcgc acgcgcagga tgtaacgctg
    10561 gcgctcggtc acggagatcg ccttgcgcgc atccagcagg ttgaacgtgt gcgaggcctt
    10621 catgacctgc tcgtaggcag gcagtgcgag ctttgcttcc agcaacttgc cgcaggcggc
    10681 ttcgtggtcg tcgaagtgcc gcagcaacac ggccgtgtca gcgtgttcga agttgtaggc
    10741 ggactgttcg acttcgttct gatgatagac atctccgtac gtgactacgc cccgaggtcc
    10801 cgcggtccac acgatgtcga agatgctctc cacgccctgc agatacatgg cgaggcgctc
    10861 gagtccgtaa gtgatttcgc cggtgaccgg cttgcagtcg agaccaccca cctgctggaa
    10921 gtaggtgaac tgcgtgactt ccatgccgtt cagccacacc tcccagccga gtccccacgc
    10981 gccgagcgta ggcgactccc agttgtcctc gacgaaccgg atgtcgtgca ccagcgggtc
    11041 gagcccgagc gcacgcagag acccgagata cagatcgagt atgggcagcg gcgagggttt
    11101 gatgaccacc tggaactggt agtagtgctg aaggcggaac gggttatcgc cgtaacgccc
    11161 gtcggtggga cggcgcgaag gctgcacgta cgccgccgac cagggttcgg gaccgatgga
    11221 cctgaggaat gtcgcggtgt ggaaagtgcc ggcacccatc tccatgtcgt acggctgaag
    11281 tatcacgcag ccctgatcgg cccagtattt ctgcagcgcg aatatcaggt cctgaaaagt
    11341 tcggggagtt gtcgaaggca ttggtcaggt gcgtgttttg gccggcgcgc agtatagcgg
    11401 ggcaccctcg aaaactactg caaattcagc gatcctgaac tccggggcac tacaggttga
    11461 cgatgcggat ctcgtacacc tcggagatgg cgttgccatc ccacgcgtac cgcaggtgca
    11521 cgctctggcg cacgttctgc accaggcgcg gccggaagat cgcgacacac tcgccgccat
    11581 tgcgtcgcac gctgtcgaag gcgattccgt tggaaccggc gtcgcgcaac tcacgcccta
    11641 gcttctgcga agcggtgtag ctcgccggat cgtagatgtc cggcatctcc atgcgccgtg
    11701 cgcgcaagtc gtgcagatcg caggcaatgt cggcgaggta gcaccgcatc tcgagctcga
    11761 tcggcggctg gctggtggcg cgcaggaagc tttcgcggtg aaagcgcgtt tcggcaatcg
    11821 cggtgtcgac ggattccgcg gcgtaatagg cgccgtagtg cccattggtg aatcgggttc
    11881 cggccggaga cagatgcgtg aacggcgcca tgacgatgga ggcgtttggt ccgctcaccc
    11941 ggtcggtcac gggcaccagg ctcaattcac ccagttccgt gcggattcgc ggattcgtcc
    12001 ttgattcgag ctcgtacagc gcgtcccagt ccgccggatc tgccacgcgc tcgaagaggt
    12061 gaatcgtcgg cagccggctc gcgacgatgc gatggcacgg cgcgaacttc accggtcgcg
    12121 cggtgtaact acgttcaacc atccttggtt tcctggctgg ttcccgtggc tgtcagccgc
    12181 gttcggtgtc gagatattgc cgcacgacgt agaggtcggc gacctggccg gacaacatgc
    12241 ggtcgagggc cgaccggcca ccgaaggtgg gcgcggtatt cggcttgcga acccactggt
    12301 cagcaagctg tggatcgggc agcaggatct gcagcgattt gtagatgccc agcaggtagg
    12361 acaggcgttc gagcgtgtcg cggggcaggc ggggattgcg ctcacgccgc catttgaaaa
    12421 aagtggaccg tgaagcgatg cctaacaact tgagctgttc tgcgacggtc aggccccacg
    12481 cctcggcgat gttcatgaag gctcgtaacc ccgcccctcc cagcgaagtg acatctgacg
    12541 tggaggactt ggggtcatgc ctcgtactgg cgacgttgag aatcatggga gtctcctaaa
    12601 tgcatatatg gactatacac gcgattaatc catacaggta cattttctcg cttcatgcga
    12661 gtgcgacgac caaaccttgg gcacatttcc ggcccagaaa cgcgcaaact cgcccgcgct
    12721 cgcaccaaat acgtgcgtgc tcggaccccg aatgcaacta aaccattgat ttagaaagaa
    12781 tcagcggctg gcacagcgaa tgcattgaga ccgaggctta aaccccttcc cagaggcaga
    12841 ggatctctaa aaatgaaacc cgcagatgta atcaagctga tcaaggacaa ggaggtcaaa
    12901 tacgcagacc ttcgcttcac cgacacgcgc ggcaaagaac aacacgtaac gatccccgcg
    12961 aacctcgtcg acgaagatct gtttcgagac ggcaagatgt tcgacggctc gtccatctcg
    13021 ggttggaagg gcatcaatga gtcggacatg atcctggctc tcgatccgac caccgcggtc
    13081 gttgatccgt tcttcgagca gaccacggtc aacatccggt gcgacatcat cgaaccggcg
    13141 accatgcagg gttacgagcg cgacccgcgt tcgctcggca agcgcgccga ggcctatctc
    13201 aagtcgaccg gcattgccga cggcgcgctg tttggtcccg agaacgaatt cttcatcttc
    13261 gacagcgtgc gctggtcgag cggcatgaac gaaacctcgt actccatcga ttccggccag
    13321 ggggcctgga acagcaacac gatctacccg gatggcaaca agggccaccg tccgaccatc
    13381 aagggcggct atttcccggt tcccccggtc gacgcgttcc aggacatccg ctccgccatg
    13441 tgcgacgcgc tcgcggaaat gggcctcaag gtcgaagtcc atcaccatga agtggccacc
    13501 ggtggccagt gcgaaatcgg tgttggtcct ggcggcctcg tgaagaaggc cgacgaagtg
    13561 cagatcctca agtacgccat ccacaacgtg gcgcacagct acggcaagac ggccactttc
    13621 atgccgaagc cgctcgtggg cgacaacggc tcgggcatgc acgtgcacca gtcgctgcag
    13681 aaagccggcg ttgcgctgtt tgcgggtgac aagtacggcg gcctctccga cacggcgctc
    13741 tactacatcg gcggcatcat caagcacgcc aaggccatca acgcattcac gaacccgggc
    13801 accaacagct acaagcgcct ggttcccggc tttgaagcgc cagtgatgct ggcgtactcc
    13861 gcgcggaacc gttcggcctc gatccgtatt ccgtgggtgt cgaacccgaa ggcgcgccgc
    13921 atcgaagtgc gcttcccgga ctcggcggcg aatccgtatt tcgcgttcgc ggccatgatg
    13981 atggccggcc tcgacggcat tcagaacaag atccatccgg gcgaaccggc cgacaaggat
    14041 ctgtacgacc tcgagccgga agaggcgaaa cacatcccga cggtgtgcca ttcgctcgac
    14101 atggccctcg agcacctcga caaggaccgc gagttcctga cgcgcggtgg cgtgttcacc
    14161 aacgacgtga tcgatgctta catcggattg aagacacagg aagtcacgcg cttccgcatg
    14221 tgcactcatc cggtcgagct cgacatgtac tacagctgtt gaggcgccgg gtgaggcggt
    14281 ggcaggcctc gggggtggca caagcgggca aaggcccgac gaatgcgccc gccagccagc
    14341 cggcctcccc gttgatcgaa aaacgggcgg cggcgtcatc gggacgccgc cgctcgaata
    14401 tgagggctac gttttggccc ggttcagccg cagggtttat gctcggctcc catgaagcgt
    14461 cagtccctag cctttttgct cgtgtcgctc tgcgcggcgt cggctgcgct ggccacgacg
    14521 tacgtgcgtg tggagaagga cggctcgaag acctattccg accggccatt gcccggcggt
    14581 cagcctatcg acctgcagcc ggctcaaacc tacagcacgc ctccaccggg ggtttcggcg
    14641 gaatcgcaac agccccgcga ggaacaactg ctccgggaac tggaagactt ccgttaccag
    14701 agctgcaccg tcactccgga aaacgacacg acatttcaga atccggaatc cgtgaacatc
    14761 gcggtttcca tcaatccgtc gctgcgtccc ggagacgtcg tcacgatgac cgtcgacggt
    14821 cagcccgcgg gtccgccgaa ctcgctgacc tacaccatgt cgcccgtgta tcgcggcaca
    14881 cacaccgtgg gtctgagcgt ggcgaacccc aaaggcaagg tggtctgcaa ttccacttcc
    14941 gtgttccacg tgcagcagcc cgggatgaat tcccccgcac gccaggcgcc gccgcggccg
    15001 cggccgccgc cgccgcgccc cacgccgcac taactaccgc atgcacggcc gggggcctgc
    15061 atggcggttg aagaacactt tctcccgtcc gcgatagctc atttcgagcc cgccgatctc
    15121 atcggcgcgc tcaccaccgg catcgtgatg ctggattcgg acctgtgcgt ggtgtacgcc
    15181 aacgtcgccg cacaggatct gctggccgtg agcgtgaacc aggcgcgcgg caaaccgttc
    15241 ctctcgctgg tgctcgaagc cgactcgctg ggcgtgctca tgcgccgcgc agtcgatagc
    15301 ggcgaaaccc tgtccgagcg tgagctcgcc gtgcagaccg gcccgctcgg ccgcgaacac
    15361 cgcgtcgtcg atgtcacgat gacgccgctc gacagcgagt tcgaccgcaa atacctgctg
    15421 ctggaaatcg ccgatgccac gcagcgtcag cgcatcacgc gcgagaacga gctgatctcg
    15481 cgtctcgaca gcagccgcca gatggtccgg cagctggctc acgagataaa gaatccgctg
    15541 ggcggattgc gcggcgcggc gcagctgctc gaacgcgagt tgcacgagca cctgcgcgaa
    15601 tacaccggca tcatcatcag tgaagcggat cggctgaccg cgctggtcga ttcgatgatc
    15661 ggaccggcgc gcgcgccgca gaagtcgctg atcaacgtgc acgaactctg cgaacacgtg
    15721 taccggctgc tgcgcagcga agcgcgcggc ggcgtgctcg tcgaacgcga ctacgatccc
    15781 agtctgccga atgccgaagt cgacgcgaac cagatcatcc aggcactgct gaacatcggg
    15841 cgcaatgccc tgttggcgat tggtgaacgc ggccgcatca tcatccgtac gcgagtggcc
    15901 gcgaacgtga acatcggcgc tactcgccac aagctcgcgg cgagcctttc ggtcgaggac
    15961 aacggtcccg gcgtgccccc cgacatcgcc gacacaatat ttttcccgct ggtcaccggc
    16021 cgcgccaatg gcacgggact aggcctcgca gtctcccagg acctcgtgac gcgccacggc
    16081 ggcctcatcg aatttgaaag caagcccggg cgcacggtgt tcaccatcct gctgccccta
    16141 ccggagaacg catgagcgtc gatcccacgt atatgcacac cacatccccg ccctccgcaa
    16201 gccagccagc cctgcgggtg tggctggtgg aagacgatgc ttcgatccgc tgggtgctcg
    16261 agcgcgcatt gcgcgccagc ggcatggttc cacgtgcgtt cgactccgca gaacccgcgc
    16321 tcgttgccct gcgcacggac gcgcccgacg tattgctcac cgacatccgc atgccgggca
    16381 gctccggcat cgacctgctg cgcgagatac gcgtgtcgca cccgacgctc ccggtgatcg
    16441 tcatgaccgc gcactcggat ctgccgagcg ccgtgtccgc ctacgaaggc ggcgccttcg
    16501 agtacctgcc caagccgttc gacatcgacc aggccgttga gctggtgcgc cgcgccggcc
    16561 agacagcgcg caagggtgac gaagttccgg cgcagacacc ggagattccg gagttgttag
    16621 gcaaggcgcc ggcgatgcag caggtgttcc gcgccatcgg ccgcctgtcg cgctcgagcg
    16681 tggccgtgct catcaccggc gagtccggca ccggcaagga actggtcgcg cgcgcgttgc
    16741 acgagcacag cccgcgccag gcgaaaccgt tcgtggcgtt gaacacgtcg gcaattcccg
    16801 cggagctgct cgaatccgag ttgtttggtc acgagaaggg ttctttcacc ggcgcagaca
    16861 cccagcgtcg cggacggttc gagcaggcca acctgggcac gctgttcctc gacgaaatcg
    16921 gcgacatgtc cacgccgctt cagacgcgcc tgctgcgcgt gctcgccgaa ggcgagtttt
    16981 accgtgtcgg cggccagaca ccgatcaagg tcgacgtgcg cgtgatcgcc gccacgcacc
    17041 agaacctgga agagcgcgtc gaacgcggca tcttccgtga agatctctat caccgcctca
    17101 acgtgatccg catcgagctg ccgccgctgc gctcacgaac cgaggacgtg cccgacttgt
    17161 tggcccacta cctgcgccag gcagccaacg agctcggcac cgagaccaag tcgctggcac
    17221 ccatcacgct cgagcgcctt caggcctatc gctggccagg caacgtgcgc gagctggtga
    17281 atctttgccg gaggctcacg gcgctggcgc cgggcagtga aatccacctg gaagatttgc
    17341 cgtcggaaat gcacatcgtc acgccggccg cgcagaccga ctgggccgcg gccctgacgc
    17401 tctgggcgaa agcacagccg cacgacggca cagtggcctg gctagaccag gcgctgccgg
    17461 aattcgaacg cgtgctcatt cgcgtggcgc tcaaccgcac gcacgggcac cgacaggaag
    17521 ccgcgcgcgt gctcggctgg gggcgcaaca cgctgacccg gaagctgaag gagctcggga
    17581 tgtcggacga cgagtcgctg ccataaacgg gtagggacag gtttcttcaa cctgtcccta
    17641 ctcaatcagc gctggcagga cagctttctt caacctgtcc ctactcaaga agactctcga
    17701 ccgggctctt ccatgaccct gcgggacaaa caacaaacgt gcgggacatc gcgttttgcg
    17761 gcgcgccccg tatgagattg atagcgctac cgttcggtag ttatgccgct tttttttgag
    17821 gtttcgtctt acggggtgac ataaccggag cggcgcgtga ttgccgtcg
//
LOCUS       DASQJS010000013         5528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_952340, whole genome shotgun sequence.
ACCESSION   DASQJS010000013 DASQJS010000000
VERSION     DASQJS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952340"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VEW08_00540"
     CDS             <1..193
                     /locus_tag="VEW08_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39238.1"
                     /translation="FCRTCGVRTFERGHLKEIGGDYVSVKIASLDDVDPKELSETAVR
                     YANGRDNKWWEPPAETRHL"
     gene            215..601
                     /locus_tag="VEW08_00545"
     CDS             215..601
                     /locus_tag="VEW08_00545"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ39239.1"
                     /translation="MKRVTGIGGIFIKSADPKRLRDWYQKHLGIDITEWGGASFHWAG
                     PHNPDGHGTTAWSVFEGSSNYFEPSAAPFMVNYRVESLHPLIAELRNEGCQVIGKVEE
                     SEFGKFGWVLDPDGNKVELWEPPPGQ"
     gene            604..1260
                     /locus_tag="VEW08_00550"
     CDS             604..1260
                     /locus_tag="VEW08_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HYJ39240.1"
                     /translation="MKITKRLSRRWLLTSGACATVAALGPLSAAEKLRRTPTQVLGPF
                     YPLEKPLDDDADLTMVKGRKARAKGQVVHVMGRVVNADGEPVKGARIEIWQANANGRY
                     THPSDDNPAPLDPNFEGYAKLLTDADGRYRFKTIKPAAYPAGDIMRPPHIHFDVAGKV
                     NRLVTQMYFPGEPLNVDDVVLAVATKNKHLLVADVRPPTPDLEPESLLARWDIVLENG
                     "
     gene            complement(1272..2093)
                     /locus_tag="VEW08_00555"
     CDS             complement(1272..2093)
                     /locus_tag="VEW08_00555"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYJ39241.1"
                     /translation="MEVATTSLLIILGVAAASIAAGTILLGRATRHGLVVRALRATAW
                     LLVFFGSLVSAAIASLQLTGRLGPAQLWFRAPSFYVAAAILLVVAAWWFRRRFVHPHA
                     HLIRYGLPALAVVFIGFVVILQRTDGRSTPISALLPTLRTAAPEITFREDGGEMRRLT
                     DYRGKVVLVNFWATWCVPCRKEMPMLSAAQSEFKDDGLVVVYLSLEEPAVLEAFLRAN
                     HFDGVQGRLTRAADYYRAGQIYPLSYLISRDGRVAKRWSGRPAEGWLRESIREEL"
     gene            complement(2129..2857)
                     /locus_tag="VEW08_00560"
     CDS             complement(2129..2857)
                     /locus_tag="VEW08_00560"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ39242.1"
                     /translation="MNSVADHYSQLLAPIYSWMSGSVEAAFEAGKAEIGEALRQLPAD
                     ALVVDLGAGFGMHAIPLARAGVRVLAVDSSTELLAELDRLAEGLPVESVADDLLSFRA
                     HLTGKAAAVLCMGDTLTHLPEHTHVDFLVQEVHEALVPGGQFVLSFRDYSAPLEGVAR
                     FIPVRSDERRILTCFLEYEEDTVVVHDILHERSGDTWDTRVSSYRKLRLSPDRVIASL
                     ETSGFEVRRQPGLRGMVRVIGTIA"
     gene            complement(2871..4394)
                     /locus_tag="VEW08_00565"
     CDS             complement(2871..4394)
                     /locus_tag="VEW08_00565"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYJ39243.1"
                     /translation="MPVPETGEALEMLAASLARARGDNFFPEMAAHLAAVLGASEVMV
                     CEAAANRRARTLGVWRRGETAPNYDYSLEGTPCAKVQAGQEIAAELSASSFPGAPQKH
                     GGYFGMPLAANDGAVLGHLAAFCDRPVSLSPSQRAICNILANRCAAELRLVHVKRERA
                     LLRGQRRQLRAEVAAAHDVDSMLGVSTAHVRVMGEIRSAALSAVNVLLRGEPGTGKEL
                     VARGIHANSTRAAKPFVKIDCAALSIDADISALPQTLALAIGGTLFLDEVGVLSFDMQ
                     ARLLAALNASQGESGGAHSFNVRIIAATNRDLFRKMREGEFLEALYDRLNLLAIDIPP
                     LRARVEDIPLLVQDMVHKRARRLGRRVQTVDPQSLSELSNYGWPGNIRELENLVERAL
                     VMQDAPVLKITTDYLGSTAPAERVALAAAAGDPGTTRTTLAGPVDFDDTLSTGLHVVQ
                     RDHILRVLDATHWVIEGNHGAALKLGLKPATLRHRMKKLGISRAANAGAHASGGEAR"
     gene            complement(4602..5057)
                     /locus_tag="VEW08_00570"
     CDS             complement(4602..5057)
                     /locus_tag="VEW08_00570"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYJ39244.1"
                     /translation="MNSFTQVLNMHVAFARFLDRFRPWLLLATRLWVSWQFWKAGWLK
                     LTTWDVTLELFRSEYQVPVLPPPLAAVCGTFGELFFPLLLVLGFFTRFGALGLFAVNA
                     MAVISYWHVLASEGYEAALAQHVLWGYMLAVIAACGAGALSLDEKLGRR"
     gene            5349..>5528
                     /locus_tag="VEW08_00575"
     CDS             5349..>5528
                     /locus_tag="VEW08_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39245.1"
                     /translation="MQLSQMWREHLQAGCPAEMRAVNIAGMSVGALDAEITACVSACV
                     THSLKMDTRIERRLHD"
BASE COUNT         1049 a         1720 c         1832 g          927 t
ORIGIN      
        1 cttctgcagg acctgtggcg tgcgcacgtt cgagcggggc cacctgaagg aaatcggtgg
       61 agactatgtg tccgtcaaga tcgcgagtct cgacgacgtg gatccgaagg aactcagcga
      121 gactgcggtg cgttacgcca atgggcgcga caacaaatgg tgggaaccgc ccgccgagac
      181 gcgacacctt tagttctggg gaggagcact ggcaatgaaa cgagtcaccg gcattggcgg
      241 catcttcatc aagtccgcgg acccgaaacg cctgcgcgac tggtaccaga agcacctcgg
      301 catcgacatc accgaatggg gcggcgcctc gttccactgg gcggggccgc acaatccgga
      361 tgggcatggc accacggcgt ggagcgtgtt cgaggggtcg tcgaattact tcgagccgag
      421 cgccgcgccg ttcatggtga actatcgcgt tgaaagcctg catccgttga tcgccgagct
      481 gcgcaacgaa ggttgccagg tcatcggcaa ggtcgaagag tccgaattcg gcaagttcgg
      541 ctgggtgctc gatccagacg gcaacaaggt cgaattgtgg gaaccaccgc cgggacaatg
      601 aaaatgaaaa tcacgaaacg tctttcccgc cgctggctgc tcacctcggg tgcctgtgcg
      661 acggttgcgg cgctgggtcc gctgtcggca gccgaaaagc tgcggcgtac cccgacgcag
      721 gtgctcggtc cattctatcc actcgagaaa cctctcgacg acgacgccga cctcacgatg
      781 gtgaagggcc gcaaggcgcg ggcgaaaggc caggtggtgc acgtgatggg ccgagtcgtg
      841 aacgccgatg gcgaaccggt gaagggcgcg cgaatcgaaa tctggcaggc gaacgcgaac
      901 gggcgctaca cgcacccgag cgacgacaat ccggcgccgc tcgatccgaa cttcgaaggg
      961 tatgcgaagc tcctcaccga cgccgacggt cgttaccgct tcaagaccat caagcccgct
     1021 gcatatcccg cgggcgacat catgcgccca ccgcacatcc atttcgacgt cgccggaaag
     1081 gtcaatcgcc tggtcacgca gatgtacttt cccggcgagc cgctcaacgt ggacgatgtc
     1141 gtcctggcgg tggcgacgaa aaacaagcac ctgttggtgg ccgacgtgcg tccaccgaca
     1201 ccggatctgg agccggaatc gctactggcg cgctgggata tcgttctcga aaacggttga
     1261 cctgccgcgg gtcagagttc ctcgcgaatc gactcgcgca gccagccctc ggctggtctt
     1321 ccactccagc gcttcgccac gcggccgtcg cggctgatca gataactcag cggatagatc
     1381 tgcccggcgc ggtaatagtc tgccgctcgt gtgagccggc cctgcactcc gtcgaaatga
     1441 ttcgcgcgga ggaacgcctc gagtacggcg ggctcctcca acgatagata gacgaccacc
     1501 agtccatcat ccttgaactc agactgcgcc gccgacagca tcggcatttc cttgcggcat
     1561 ggtacgcacc acgtcgccca gaagttcacg agcaccacct tgccgcggta atccgtgagc
     1621 ctgcgcattt cacctccgtc ttcccgaaaa gtgatttccg gagctgccgt acgcagcgtg
     1681 ggcagcaacg ccgagatggg agtcgatcgg ccatccgttc gctgcagaat cacgacgaac
     1741 ccgatgaaga ccaccgccag cgcgggcagg ccgtatctga tgaggtgcgc gtgcgggtga
     1801 acgaagcggc gccggaacca ccaggcggca accaccagca ggatcgccgc ggcgacatag
     1861 aacgacggtg cgcggaacca cagctgcgcg ggccccagtc gtcccgtcag ctgcaaactt
     1921 gcgatcgcgg ccgagacgag cgagccgaag aaaacgagca gccaggcggt ggctcgcaag
     1981 gctcgcacga caaggccgtg ccgtgtggcg cggcctaaca atatggtgcc ggcagcaata
     2041 ctcgcggcgg ccacgccgag gatgatcagc agcgatgtgg tggcgacttc catgacacgc
     2101 gcattgtgtc accgccccct gtaggacttc aggcgatggt gcctatgacg cggaccattc
     2161 cgcgcagtcc gggctggcgg cgcacctcga agccgctggt ttcgagactg gcgatcaccc
     2221 ggtccggtga caaccgcagc tttcgataac tgctgacacg cgtgtcccag gtatcgccgg
     2281 agcgctcgtg caggatgtcg tgcacgacca cggtgtcttc ttcatattcg aggaaacacg
     2341 tgaggatccg gcgttcgtcg ctgcgtaccg ggatgaatcg cgcgacacct tcaagcggcg
     2401 cgctgtagtc gcgaaacgag agcacgaact ggccgcccgg gaccagcgcc tcgtgaacct
     2461 cctggaccag gaaatccacg tgagtgtgtt ccggaagatg tgtgagcgtg tcgcccatgc
     2521 aaagcaccgc ggcggccttg ccggtgaggt gcgcgcgaaa cgacagcagg tcgtcggcca
     2581 ccgattccac gggcaggcct tccgcaagcc ggtcgagttc ggccagcagc tccgttgaac
     2641 tgtcaacggc cagcacgcgc acgccggcgc gtgccaacgg gatggcgtgc ataccgaagc
     2701 ccgctccgag atcgaccacc agcgcgtcgg cgggcaactg ccggagcgct tcgccgattt
     2761 cggctttgcc ggcttcgaac gcagcctcca cgctgccgga catccaggag tagatgggcg
     2821 cgaggagctg cgagtagtga tcggcgacgg agttcatgag ccggagttgc ttaccgggct
     2881 tcgccgccgg aggcgtgcgc gcccgcgttc gcagcgcgcg agattcccag cttcttcatg
     2941 cggtggcgca gcgtcgcggg tttcaggccg agtttgagcg cggcgccgtg gttgccctcg
     3001 atgacccagt gcgtggcgtc gagaacgcgg aggatgtggt cacgctgcac gacatggaga
     3061 cccgtgctca acgtgtcgtc gaaatccacg ggccccgcga gcgtcgtgcg cgtggtgccg
     3121 gggtcaccgg ccgcggcagc gagcgccacc cgctcggcag gggcggtgct cccgagataa
     3181 tccgtggtga ttttcagcac gggcgcatcc tgcatgacca gggcgcgttc gacgagattc
     3241 tcgagctcgc gaatgttgcc gggccagccg tagttagaga gctcggacag ggactgcgga
     3301 tcgaccgtct gcacccggcg gcccagccgg cgggcgcgtt tgtgcaccat gtcctgtacg
     3361 agcaggggga tgtcttcgac gcgggcgcgc aacggtggaa tgtcgatggc cagcaggttg
     3421 agccgatcgt agagcgcttc caggaattca ccttcgcgca ttttccggaa caggtcgcga
     3481 ttggtggcgg cgatgatgcg cacgttgaaa ctgtgggcgc cacccgactc gccttgtgag
     3541 gcgttcaggg cagccagcaa acgggcttgc atgtcgaagg aaagaacgcc gacttcatcg
     3601 aggaacagcg tcccgccgat ggccaatgcc agcgtttgcg gcagagcact gatgtccgca
     3661 tcgatggaca gcgcggcaca gtcgatcttc acgaatggct tcgcggcgcg cgtgctgttc
     3721 gcgtggatgc cccgtgcaac gagttccttg ccggttccgg gttcgcctct cagcagcaca
     3781 ttgacggccg acagcgcggc gctgcggatt tcgcccatga cgcgcacgtg cgccgtgctc
     3841 acgccgagca tgctgtcgac gtcgtgcgcg gcggcgactt ccgcgcgcag ttgccgtctc
     3901 tgtccgcgca gcagggcgcg ttcgcgtttt acgtgcacga ggcgcagttc ggccgcgcag
     3961 cggttcgcca ggatgttgca gatggcgcgc tgggaagggc tgagcgatac cggtcgatcg
     4021 cagaaggctg ccaggtggcc gagaaccgcg ccgtcattcg ccgcgagcgg catgccaaag
     4081 tagccgccat gtttctgtgg tgctcctggg aacgagctcg cgctcagctc ggccgcgatc
     4141 tcctggccgg cctgcacctt tgcacagggc gttccctcga gactgtaatc gtagttaggt
     4201 gctgtctcgc cgcggcgcca gacccccaga gtgcgtgcgc gccggttggc ggcggcttcg
     4261 cagaccatga cttcgctcgc gccgagtacc gcggcaagat gcgcggccat ctccgggaaa
     4321 aaattgtctc cgcgagcgcg agccaggctc gcggccagca tttcgagagc ctctcccgtt
     4381 tccggcaccg gcattgcagc aacactctat taaggagtgc gcgagtatag gcgacctgcc
     4441 gattcgtggc gaagcgaggt tggcaacccc cggaacccgg cggcgcatga acgccgcggc
     4501 gctcggccag gcgatgcctc gcaatcgtgc actgcccggt gcggcgggca aatgaaggtc
     4561 aactgcggcg atgccgacgg cgcggggtgt tcccgccgct gttaccggcg gcctaacttc
     4621 tcgtcgaggg acaaggcgcc ggcgccgcat gcggcgatca ccgccagcat gtaaccccag
     4681 agcacatgtt gcgcgagtgc ggcctcgtag ccttcgctgg caagcacgtg ccaatacgag
     4741 atcacggcca tcgcgttgac ggcgaatagt ccgagggcgc cgaatcgcgt gaagaatccc
     4801 agaacgagca gcaacggaaa aaacagttca ccgaacgtcc cgcacaccgc ggccagcgga
     4861 gggggcagca ctggcacctg gtactcgctg cgaaagagct cgagcgtgac gtcccaggtc
     4921 gtgagcttga gccagccagc cttccagaat tgccagctca cccagaggcg agtggccagc
     4981 agaagccacg ggcggaatct gtcgagaaag cgcgcgaagg ccacgtgcat gttcagtact
     5041 tgcgtgaagc tgttcatcga gactccctgg tgttgttagc gctttagacc ggggacaagt
     5101 cccgtttatt gcgaacggca accggggttc gacatggcat gtacgcaacg cccagccccc
     5161 ggcgtgaggg tgcccggaat caactactta agggcgttcc gtggcggtga aatccggcgt
     5221 gtgggatcgc tgccacggat cacggatcac tgaagtcggc aggagtgccg cggcacggga
     5281 ttttccgcgg gacgcttgca gcaatttggg gtactttcgc ccgcaatcca ggcttgggag
     5341 cgaaagcgat gcaactgtcg caaatgtggc gagagcatct gcaggcgggc tgtccggccg
     5401 aaatgcgcgc cgtgaatatc gcgggtatgt cggtgggtgc actggatgcg gaaatcacgg
     5461 catgcgtgag tgcctgcgtg acccactcat tgaagatgga cacgcgaatc gagcggcggc
     5521 tgcatgac
//
LOCUS       DASQJS010000014        10998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4092522, whole genome shotgun sequence.
ACCESSION   DASQJS010000014 DASQJS010000000
VERSION     DASQJS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10998
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4092522"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..297)
                     /locus_tag="VEW08_00580"
     CDS             complement(73..297)
                     /locus_tag="VEW08_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HYJ39246.1"
                     /translation="MRTIRLKVKPGSREEELSELEDGSWLARVKAPPVDGKANAAVVA
                     LVAKHFGLRKAQVHIKSGATGRSKLVQLED"
     gene            complement(362..877)
                     /locus_tag="VEW08_00585"
     CDS             complement(362..877)
                     /locus_tag="VEW08_00585"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYJ39247.1"
                     /translation="MPPVAQASLKPEVNSTTHIHQIYIKAEPGQIWDAITSPEWNGKY
                     GYRGVSHYDLKPGGKFRCIANEQMRSFGLPEVIVDGEVIEVKPPYKLVQTYRFLFNEE
                     HRKEGFTRISFEIEQTAGGFCRLTITHDTAGAPMMDRATKSNFSTDGGGGWDWILSDL
                     KTLLETGKTMS"
     gene            complement(850..1194)
                     /locus_tag="VEW08_00590"
     CDS             complement(850..1194)
                     /locus_tag="VEW08_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ39248.1"
                     /translation="MVDEDLVFKALADSSRRFLLDLLHKRDGQTLGELEWELEMTRFG
                     VMKHLKVLEEAGLIVTRREGREKLHFLNAVPIRLIHDRWIDKYKERELSALADLKTTL
                     EKTKCPPLRKRA"
     gene            complement(1266..3251)
                     /locus_tag="VEW08_00595"
     CDS             complement(1266..3251)
                     /locus_tag="VEW08_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HYJ39249.1"
                     /translation="MSWFRLSLVAAVVLSAGAASATSVVPSFQVASPDGRIVVAFSLA
                     DARASYTVSVGGKEILRQSRLGVARDDADFTSNIDVTANFEKRAAKLEKVEDRYELLT
                     SKRRQNVYRAHRRVMEVQTPSGARMDIEFQVSNDGFAFRYVFPETDAKVHKISREASS
                     FNFLAGTRGWLQPIAPARSGWNESNPSYEEYYERDIPVGQPSLQGGAWVFPALFRTTV
                     GGGDTWLLVSETGLRRNYCGSRLLAARRSSEYFIDFPGPLETSNGGAVTPESTLPWTT
                     PWRLVVIGDLKTIAESTLGTDLADAPAKGLKTTAPGPGKASWSWPLLGDDNTVVKVQK
                     RFVDYAAEMGWQYTLVDSAWDRQIGYDGLKELVEYGRPKNVKILIWYNSAGEWNTTPL
                     TPRDRMYDPATRRAEFQKIKDLGIAGVKVDFFAGDAQSTIAYYHDILEDAARIGLAVN
                     FHGATLPRGWQRTYPNLMTMESVRGLEYNTFEQRNAEESPVHDAMLPFARNVFDPMDF
                     TPVVLDRLNNIVERRTSAAFELATAVLFTSGIQHYAEIPQGMAKAPVYVRNFLKHVPA
                     IWDDVKFIDGFPGQYVVVARRAGKAWYVAGINADRQQPRKVKLDLKALGVMKQGTLIT
                     DGIDFLGFKSETFPLEKGVFTEEITMRPRGGFVLTFE"
     gene            3250..4119
                     /locus_tag="VEW08_00600"
     CDS             3250..4119
                     /locus_tag="VEW08_00600"
                     /inference="COORDINATES: protein motif:HMM:NF039735.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6348 family protein"
                     /protein_id="HYJ39250.1"
                     /translation="MSYLPKWRKPSSGVPSPDMFSWFRRPDRPPPPAAENPGRGHTVR
                     VAFANAQRSWEEEDDLAASLAATLNSMGHKASVKHDWVELEGGFSLVPQVVDVKPMDS
                     SGVQTVTTIQISHPVLVPGGVFEFQHSTGDDVRDSFAQGFKAWAELDLPVFQDAQRDE
                     VTVCMFMQMSPGQESTSFLAPGRRLVLGPPVQMTQKTEPMPGEHSFCPCCLFTQSIGA
                     FDDLVKGKAFCGVRLFVMRDADGHIDADCRVNGIDRPEGVEALKRYAQTWPDRGTEFR
                     KQYVCIQTREPGK"
     gene            complement(4230..4934)
                     /locus_tag="VEW08_00605"
     CDS             complement(4230..4934)
                     /locus_tag="VEW08_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39251.1"
                     /translation="MNVTAHRSIGPAPQPGPDPQPDAPGQPGPKPPPKHLSARALTAL
                     VSQLTTGYPQPDGPEDPPGPLDPYIRKALERSVIGAGTNGYLWRVIADKHPEIWDVIG
                     GDPGTQVSLNPQPLPPRSAFLAAVILEFTERMTIVAEIADLIPRPGGERGIIIVGGHV
                     ARFVDDICGNGLHIKWPLPWPAPPWFSDVLSGADLIVMGTQFQQSAVIAMDRDLGRTF
                     ADAAHALLEAGAARLV"
     gene            5051..7294
                     /locus_tag="VEW08_00610"
     CDS             5051..7294
                     /locus_tag="VEW08_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HYJ39252.1"
                     /translation="MDRREIRFDGWKVDFDSGEISRDGNTHRLQDQPLQILDELVRHP
                     GELVTREQLISRLWPTGVVEFDTGLNSAMRKLRIALGDDAETPRYIETLPRKGYRFIA
                     RIEDDAPISGIPTLTSQYVPTTFETGAVIGRRASDRRAPVKRLALGFGSIFAALILAA
                     VAWYMPGNLFQADADADSESLPTIVVLPLVDMSVDQGESALCDGLTEELSNWLAHIPT
                     LRVVARTSAFAFKGQNIDVRKIAAELGASHVLEGSLRRSGDQLRITVQLIAADTGLHI
                     WSRSFDRPIGDIFEIEDTVSRAVAEQLHLELTADTAELWAQRQPGTMEAHELYLLGRA
                     RQSRRTADDNLKSVEYLRRAVQADPQYVPALVALSESLLNGISLNRAPIEDVKAEVEP
                     LINRAMQINPDLPEVLAVKGWLLNEEFRADEALPLLLRATRLNPNDASSHRFLGDLYD
                     RLAEPDEAMKHFSAAARLDPMDFISHVFRCMELVDLGEYTEATAACTRARELDPTHMW
                     GPLATSWIARAQGKTDEAMQWIDAARKLQPRDTYLVDQKIELLLAQEKFADARTVMRE
                     LPADQSFFSLARESNIIYAESGGGAVKEWMAGHDVTSRAGTGLELIELARMQLNSGDP
                     VTARATLAHAERVLPRSSADMFDGGQIRHEYSVALLLAAIELKSGGDQARAMKLLHDL
                     DRMLDRYEENGGKHFGVYRLRAESLAMQGKTAEAQAALNTAWKKGWRTLWRARRESYF
                     AGIEMPK"
     gene            complement(7425..8273)
                     /locus_tag="VEW08_00615"
     CDS             complement(7425..8273)
                     /locus_tag="VEW08_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYJ39253.1"
                     /translation="MYHVTLRGNHQQDIFLEAEDPHRMNALVAETMDRFGARLHAYCY
                     MSNHIHVLVQIGDVPLGKIMLSIASRYARATQTRLQTTGHFFERRYYSVLVDVEQYFL
                     AVLRYIHLNPVAAGLAASPDAYPWSSHHAYSATRVEPWVTTDFALSLFSTDRMRAIHD
                     YRKFVAQAPMGVPEVSPLDESNPGDRRVLGSDEFVQGLLGAQWKPRSKKSLETLVGEA
                     CVHFSCTGAEMASISRKSNLVAARAWVVQQAVAERIASIAAVARRFNRDESSLRHAMK
                     AHALKR"
     gene            complement(8359..8697)
                     /locus_tag="VEW08_00620"
     CDS             complement(8359..8697)
                     /locus_tag="VEW08_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39254.1"
                     /translation="MNFSAQVRELAIAYGLTAVAMLIGLLLMRADRRAYFRWPVYSAM
                     AMALGVVLWNLVRKHLLPPDWPITHPGTLYYSALTLYFVIGLGLGLLLGRLTRGAGPG
                     NRENSNSDGK"
     gene            complement(8694..9470)
                     /locus_tag="VEW08_00625"
     CDS             complement(8694..9470)
                     /locus_tag="VEW08_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39255.1"
                     /translation="MSPDSKRKRGIAMAIILIGALVATFSRREAAHTPPATEKSRADK
                     QDKTTPASAAFDFYLMAMTVHPAFCADNARRRECASGNSRPLVIHGLWPERMEPRTYP
                     HDCPAPALDLDPALSLELADMMPGMADGLHHHEWRTHGGCSGLDDDDYYRHALELARR
                     VDGALGATLTTHAGGETTAGELRGIADMYSPGLGRTLTFHCRTLRDAGNRRPYLIEVR
                     QCVDNDGPGGAPGTSLACAIVKRRDQGCGQSFMIAGEVAR"
     gene            9548..>10998
                     /locus_tag="VEW08_00630"
     CDS             9548..>10998
                     /locus_tag="VEW08_00630"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYJ39256.1"
                     /translation="MKPSVLISRLAVMFVAGISAVSSQTLELHQADRQPLPVIDPASD
                     DATLAIQRFSLPKGMQAKLWAAEPMLANPVAFDFDEKGRLFVSETYRYRSSVLDIRDY
                     MGMLELDLASRTIEDRTALHRKVFGAQAKDFAIEGEVVRLVEDTNGDGVADKSRAYAD
                     GFNTELDGIASGVLARHGKVWFTNIPSLWLLEEGTDGKLKKDELLRGFGVRYNYTGHD
                     FHGLAMGHDGKIYYSIGDRGTHVKGKEGQVVGYPDEGAVFRSNPDGTEFEMVARGLRN
                     PQELVFDEHGNLFTGDNDSDQGDMERLVYLVEGGDSGWRIGYQHAPLGKGGPWMREGL
                     WKPEFAGRPAYLLPPVCNIEDGPSGLTYYPGTGLDPRYAGHFFITHFKGSIARSGVQT
                     YTLKQNGATFTPTSSQQFMGGVLPTDVTFAPDGKLYILDWVDGWPKSNKGRVYGIAPA
                     NPEPAQAKVSAELTKLLKDGFTQRNDQELAALLS"
BASE COUNT         2051 a         3467 c         3444 g         2036 t
ORIGIN      
        1 ccttcatgac gaacgggctg gcatcgggca gtccgaacaa cgggccgaag gtatacagcg
       61 tgatcacggt agttagtcct ccaactgcac gagcttggag cggccggtag cgccgctctt
      121 gatgtgcacc tgggccttgc gaagtccgaa atgcttcgcg accagcgcga cgaccgccgc
      181 gttggccttg ccatctaccg gaggcgcttt cacgcgcgcg agccagctgc cgtcctcgag
      241 ctccgacaac tcttcttcgc gtgagcctgg tttgaccttg agcctgatcg ttcgcatcgc
      301 ccgtcccggt gccgcgacat tatgcggcac cgggcggacg atagttcgat cgaaacggct
      361 gctacgacat cgtcttgccg gtctccagca gggtcttgag gtcgctgagg atccagtccc
      421 atccgccgcc gccgtcggtg ctgaaattgg acttggtcgc gcgatccatc atcggcgcac
      481 cggcggtgtc gtgcgtgatg gtcaggcggc aaaagccgcc cgcggtttgt tcgatctcga
      541 atgagatgcg tgtgaacccc tccttcctgt gttcctcgtt gaacaggaag cgataggtct
      601 gcaccagctt gtacggcggc ttcacttcga tgacttcgcc gtcgacgatg acttccggca
      661 ggccgaaact gcgcatctgc tcattggcga tgcagcggaa ttttccaccc ggcttcaggt
      721 cgtagtggct gacgccgcga tatccgtact tcccgttcca ctcggggctg gtgatcgcgt
      781 cccagatctg acccggctcc gccttgatgt agatctggtg gatgtgcgtg gtcgaattga
      841 cttccggctt caggctcgct tgcgcaacgg ggggcatttg gttttctcca atgtggtttt
      901 cagatccgcg agcgcggaca actcgcgctc cttgtatttg tcgatccagc ggtcgtgaat
      961 gagacggatg ggcacggcat tgaggaagtg cagcttctcg cggccttcgc gccgcgtgac
     1021 gatgaggccg gcctcttcca gcaccttgag gtgcttcatg accccaaagc gggtcatctc
     1081 cagttcccat tcgagctcgc ccaaagtctg gccgtcacgc ttgtgcagca gatcgagcag
     1141 gaacctgcgc gatgaatccg ccagagcttt gaacaccaga tcttcgtcga ccatggcgcg
     1201 gactatatgt gaccttttgg tcacatgtca aaaccagcgc tttcgcggct gtttttcagt
     1261 gaactttatt cgaacgtgag cacgaaccca ccgcgcggcc gcatcgtgat ttcttctgtg
     1321 aacacgccct tctcgagcgg aaaggtctcg ctcttgaacc cgaggaagtc gatgccgtcg
     1381 gtgatgagcg tgccctgctt catcacgccc agcgccttca gatccaactt cactttgcgc
     1441 ggctgctgcc ggtcggcgtt gatgccggcg acataccagg ccttgcccgc gcggcgcgcg
     1501 accaccacgt attgacccgg aaatccgtcg atgaacttca cgtcatccca gatcgccggc
     1561 acgtgcttca ggaaattccg cacgtagaca ggcgccttcg ccatgccttg cggtatttcc
     1621 gcgtaatgct ggatgcccga cgtgaacaac accgcggtgg cgagttcgaa tgcggcggac
     1681 gtgcggcgtt caacgatgtt gttgagccga tccaggacca cgggcgtgaa gtccatggga
     1741 tcgaacacat tccgcgcgaa cggcaacatc gcgtcgtgca cgggcgattc ttccgcgttg
     1801 cgttgctcga aggtgttgta ttcgaggccg cgcaccgact ccatggtcat cagattcgga
     1861 tacgtgcgct gccagccgcg cggcagcgtg gcgccgtgaa agttgaccgc aagtccgatg
     1921 cgcgcggcgt cttcgagaat gtcgtggtag tacgcgatcg tcgactgcgc atcgcccgcg
     1981 aagaaatcca ccttcacgcc ggcaatgccg agatccttga tcttctggaa ctctgcgcgc
     2041 cgcgtcgccg gatcgtacat gcgatcgcgc ggcgtcagcg gcgtggtgtt ccactcgccg
     2101 gccgagttgt accagatgag aatcttcacg ttcttcggcc gcccgtattc gacgagctcc
     2161 ttcaaaccgt cataaccgat ctgccggtcc caggccgagt ccaccagcgt gtactgccaa
     2221 cccatctcgg cggcatagtc cacgaagcgc ttctgcactt tgaccacggt gttgtcgtcg
     2281 cctaacaacg gccagctcca cgaggccttg ccgggaccgg gcgccgtggt cttgaggcct
     2341 ttcgcgggcg catcggccag gtcggtgccg agcgtcgact ccgcgatggt tttcagatcg
     2401 ccgatgacca cgagccgcca cggagtagtc cacggcaacg tggattccgg tgtgaccgcg
     2461 ccgccgttgc tggtctcgag cggaccgggg aagtcgatga aatattcgct gctgcggcgc
     2521 gccgcaagca gacgcgagcc gcagtagttg cgccgcaagc cagtctcgct taccagcagc
     2581 catgtatcgc ccccaccgac ggtagttcgg aacaacgccg ggaacaccca cgcgccgccc
     2641 tgtagcgagg gctgacccac gggtatgtcg cgctcgtagt actcctcata tgaagggttc
     2701 gattcattcc agcccgaacg cgccggtgcg atgggctgca gccagccgcg ggtgccagcg
     2761 aggaaattga acgacgacgc ctcgcggctg atcttgtgca ccttggcgtc ggtctcggga
     2821 aacacatacc gaaacgcgaa gccgtcgttc gacacctgga actcgatgtc catgcgcgcg
     2881 cccgacggcg tctgcacttc catgacgcgc cggtgggcgc gatacacgtt ctgccggcgc
     2941 ttcgacgtca gcagctcgta gcggtcttcg accttctcga gcttcgccgc gcgcttctcg
     3001 aagttggccg tcacgtcgat gttgctggtg aaatcggcat cgtcgcgggc cacacccagg
     3061 cgcgactggc gcaggatttc cttgccgccc acgctcacgg tgtagctggc gcgcgcgtcg
     3121 gccagcgaga acgcgacgac gatgcgtccg tcagggcttg caacctggaa cgaaggcacc
     3181 acggaggttg ccgaagccgc accggcgctc aacaccaccg ccgcaaccag tgacagacga
     3241 aaccacgaca tgagctacct cccgaagtgg cgcaagccta gcagcggtgt accctcaccc
     3301 gacatgtttt cctggttccg caggcccgac cgaccgcctc ctcccgccgc cgagaatccc
     3361 ggccgtggcc acaccgtgcg cgttgcattc gccaatgcac agagatcctg ggaagaagag
     3421 gatgacctgg cagcctcgct ggccgccacc ttgaactcga tggggcacaa ggcatccgtg
     3481 aagcacgact gggtggaact cgaaggcgga ttctcgttgg taccgcaggt cgtggatgtc
     3541 aaacccatgg acagcagtgg cgttcagacc gtgaccacga tccagatctc acaccccgtg
     3601 ctggttccgg gcggggtttt cgaattccag cactccacgg gcgacgacgt tcgcgactcg
     3661 tttgcccagg gcttcaaggc gtgggcggaa ctcgacctgc ccgttttcca ggatgcgcag
     3721 cgggacgagg taacggtgtg catgttcatg cagatgtcgc cgggccagga gtccacgtcg
     3781 tttctcgcgc ccggccgccg tctcgtgctc ggacctccgg ttcagatgac gcagaaaaca
     3841 gagccgatgc ccggtgagca ctccttctgt ccgtgctgcc tgttcacgca aagcatcggc
     3901 gccttcgacg atctcgtgaa gggcaaggcg ttctgcggcg tgcggctctt cgtcatgcgc
     3961 gacgcagatg gccatatcga tgccgattgc cgcgtgaacg gcatcgatcg gccagagggt
     4021 gtggaagcgc tcaagcgtta tgcgcagacc tggccggatc ggggcaccga atttcgcaag
     4081 cagtacgtct gcatccagac gcgcgaaccg gggaagtagt tagagacccg cgaggtcggc
     4141 cagcacgtca cggtgcccgg gggtagaaca ccgtgacggc gggccagaga ggacatccgt
     4201 gccccctcct tctccactga acgattcggc taaacgagtc tggcggctcc cgcttcgagc
     4261 agcgcatgcg ccgcgtcggc gaaagtgcgg ccgagatcgc ggtccatcgc aatcacggcc
     4321 gactgttgaa actgtgtgcc catgacgatg aggtcggcgc cgctcaacac gtccgagaac
     4381 cacgggggcg cgggccaggg caacggccac ttgatgtgaa gtccatttcc acagatgtcg
     4441 tcgacgaaac gcgccacgtg accacccacg atgatgattc cgcgctcgcc gcccggccgc
     4501 ggaatgagat cggcgatctc cgcgacgatg gtcatgcgtt cggtgaattc gaggatgacg
     4561 gcggcgagga aggcagacct cggcggcagc ggctgcggat tcagcgacac ctgggtgccc
     4621 ggatcgcctc cgatgacatc ccatatttcc ggatgtttgt ccgcgatgac acgccagagg
     4681 taaccattcg tgccagcgcc gatcaccgat cgctccagcg ccttgcgaat gtaggggtcc
     4741 agggggccag gaggatcctc cgggccgtca ggttggggat aacccgtcgt caattggcta
     4801 actaacgccg tcaatgcccg ggcggaaaga tgcttgggcg gcggcttggg accaggctga
     4861 cccggtgcgt ccggctgcgg atcgggacct ggctgtggag ccggcccgat gctgcgatgc
     4921 gcagtgacat tcattgttga cctccgtgaa attgcttttg tgcgtgcttt cggggcggag
     4981 actcgccctg tggccctccg gaggcgatgg acggacgctg atcagtggct gacttgtcta
     5041 tgatccgcac gtggacagac gtgaaatccg gttcgatgga tggaaagtgg acttcgattc
     5101 gggagaaatc tcgagggacg gcaataccca tcgcctccag gaccagcccc tgcagatcct
     5161 cgatgagctg gtcaggcatc ccggtgagct cgtcacccgc gaacagctca tatcccggtt
     5221 gtggcccacc ggcgtggtgg aattcgacac cgggctgaac agcgccatgc gcaagctgcg
     5281 catcgccctc ggcgatgacg ccgaaacccc ccgttacatc gaaacgttgc cccgcaaggg
     5341 ataccggttc attgcccgga tcgaggacga tgcccccatt tcgggcatcc cgacgctgac
     5401 gtcgcagtac gtccccacga cctttgaaac cggggcggtc atcgggcgcc gggcttcgga
     5461 ccggcgggcc cccgtaaagc gcctggccct cggattcggc agcatctttg ccgcgctcat
     5521 cctggcggcg gtcgcctggt acatgcctgg aaatctgttt caggccgatg cagatgccga
     5581 ctccgagtcg ttacccacca tcgtggtgtt gccgctggtc gacatgagcg tcgatcaggg
     5641 cgaatcggcg ctgtgcgacg gactgaccga agagctgtcg aactggctgg cgcacatccc
     5701 cacgttgcgg gtcgtggccc gcacatccgc gttcgcgttc aaggggcaga acatcgatgt
     5761 acgcaagatc gcggccgaac tcggcgccag ccacgtgctc gaaggaagtc tgcggcgctc
     5821 cggggaccag ttgcgcatca ccgtgcagct catcgcggct gacaccggcc tgcacatctg
     5881 gtcgcggtcc ttcgatcgcc ccatcggcga catcttcgag atcgaggaca ccgtctcgcg
     5941 cgccgtggcc gagcagttgc acctggagct caccgcggat accgccgaac tgtgggcgca
     6001 gcgccagccg ggaacgatgg aagctcacga gctgtacctg ctcggccgcg cgcggcaaag
     6061 ccggcgcacc gcggatgaca atctgaaatc ggtggaatat ctccgccgcg cggtgcaagc
     6121 cgatccgcaa tacgtgccgg cgctggtcgc cctgtcggag tcgctgctca acggcatttc
     6181 gttgaatcgc gcccccatag aagatgtgaa ggccgaggtg gagccgctga tcaaccgcgc
     6241 catgcagatc aatccggacc tgcccgaggt cctcgcggtg aaaggctggc tgctcaacga
     6301 agaattccgc gccgacgaag cgctgccgct gctgctgcgc gcgaccaggc tgaatccgaa
     6361 cgacgcctcg agtcatcgtt tcctcggaga tctgtacgac cggctggcag agcccgacga
     6421 ggccatgaag cacttctcgg ccgccgcgcg gctcgacccg atggatttca tttcgcatgt
     6481 gttccgctgc atggaactgg ttgatctcgg cgaatacacc gaagccaccg ccgcgtgcac
     6541 gcgcgcgcgc gagctcgacc cgacgcacat gtgggggccg cttgccacct cgtggatcgc
     6601 gcgtgcgcaa ggcaagaccg atgaggcgat gcagtggatc gacgccgcgc gcaagctgca
     6661 gccgcgggac acctacctcg tcgatcagaa gatcgagctg ttgctggcgc aggaaaaatt
     6721 cgccgacgcg cgcaccgtga tgcgcgagtt gcctgcggat cagagcttct tctcgctggc
     6781 tcgcgaatcg aacatcatct atgcggagag cggcggcggg gccgtgaaag aatggatggc
     6841 cgggcacgac gtcacgagcc gggcgggcac tggcttggag ctcattgaac tcgcgcgcat
     6901 gcagctcaac tccggggacc cggtgacggc gcgcgcgacg ctggcccatg ccgaacgcgt
     6961 gttgccgcgg tcgagcgcgg acatgttcga cggtggccag atccgccatg aatattcggt
     7021 ggcgctgctg ctggccgcca tcgaactcaa gagcggcggc gaccaggcgc gcgccatgaa
     7081 actcctgcac gatctcgatc gcatgctgga tcgatatgaa gaaaatggcg gcaaacactt
     7141 cggggtgtac cgcctgcgtg ccgagtcact cgccatgcaa ggcaagacgg ccgaggcgca
     7201 ggcggcattg aacaccgcct ggaaaaaagg ctggcgcacc ctatggcgcg cgcgccgcga
     7261 atcttatttc gcgggaattg agatgccgaa gtagctaatt gggcttcggc ggcggacgcg
     7321 ggcctggcgg catcgttcac tccactgaga tttcaaacgc tggggcggtt ttacgccgaa
     7381 tgagcgcttt cgtaaacggt gccgggccgg gaaaccggga aaactcagcg cttcagcgca
     7441 tgcgctttca ttgcgtgcct caaactcgat tcatcgcgat tgaatctgcg ggccaccgcg
     7501 gcgatcgatg caatgcgctc agctaccgcc tgttgcacaa cccaggcccg cgcggcaacg
     7561 aggttggatt tccggctgat ggaagccatt tccgcgccag tgcagctgaa atgcacgcat
     7621 gcctcgccaa ccaaggtttc cagtgacttc ttcgacctgg gcttccactg cgcccccaac
     7681 aggccttgca caaactcatc gctgccgagg actcgccgat cacccggatt gctttcatcg
     7741 agtggcgata cttcgggcac acccattggt gcttgcgcca caaactttcg atagtcgtgg
     7801 atcgctcgca ttcgatccgt actgaacaat gacagcgcga agtcagttgt tacccatggc
     7861 tcaacccgcg ttgctgaata cgcatgatgg ctcgaccatg gataggcgtc gggtgatgca
     7921 gcgaggccgg ctgccacagg attgaggtgg atgtaacgca acacagcgag gaaatactgt
     7981 tcaacatcca ccaggaccga gtagtagcgc ctctcgaaga aatgtcccgt ggtttgcaaa
     8041 cgagtttgcg tggcgcgcgc gtagcggctc gcgatgctca acattatctt gcccagcggc
     8101 acatcgccga tctggacgag cacgtgaatg tgattgctca tgtagcaata cgcatgcaac
     8161 cgggcgccga atcgatccat ggtttcggcg acgagtgcat tcattcgatg tggatcttcc
     8221 gcctcgagga agatgtcctg ctggtggttg ccacgaagag tgacgtggta catcgcaccg
     8281 ggtgcatgga gcctcagtgg ccttcccatg cgtaaaactt atgctggcag gcgcgcagcg
     8341 ggcagaccga aaaactgcct atttgccatc actattcgag ttttcccggt ttcccggccc
     8401 ggcacctcga gtcagacgtc cgagcaagag ccccagcccg agtccgatca cgaaatagag
     8461 cgtgagcgcg ctgtaataca gcgtgccagg gtgcgtgatc ggccagtcgg gcggcaacaa
     8521 gtgcttgcgt accaggttcc acagtacgac accgagcgcc atggccatgg ccgagtagac
     8581 aggccagcgg aaatacgcgc gccggtcggc gcgcatgagc agcaggccga tcagcatcgc
     8641 caccgcggtg agtccatagg caatggccag ttcgcgcacc tgcgcggaga aattcatcgt
     8701 gccacctctc cggcgatcat gaatgactgg ccgcaaccct ggtcgcgccg cttcacgatg
     8761 gcgcacgcga gcgaggtgcc gggggcgcca cctgggccgt cgttgtcgac gcactggcgt
     8821 acttcgatga gatagggtcg ccggttcccg gcatcgcgca acgtccggca atggaacgtg
     8881 agcgttcgcc cgagccccgg gctgtacatg tcggcgattc cgcgcagctc gcccgcggtg
     8941 gtctcgccgc ccgcgtgggt agttagtgtc gcgccgagtg cgccgtccac gcggcgtgcc
     9001 agctcgagtg cgtgccgata gtagtcgtcg tcatcgagac cgctgcagcc accatgcgtt
     9061 cgccactcgt gatgatgcag tccatcggcc atgccgggca tcatgtcggc aagctcgagc
     9121 gacagcgccg ggtcgagatc gagagccggc gcgggacaat cgtgcggata cgtgcgcggc
     9181 tccatgcgct cgggccacag accgtgaatc accagcggtc gtgagttgcc gctcgcacat
     9241 tcgcggcgac gggcattgtc ggcgcagaac gccggatgca cggtcattgc cattagataa
     9301 aagtcgaagg cggcactggc cggcgtggtc ttgtcctgct tgtccgctcg ggacttttcc
     9361 gtggccggcg gcgtatgagc cgcttcgcgc cgtgaaaacg tcgccaccag cgcgccgatc
     9421 aatatgatcg ccatcgcaat cccgcgtttg cgcttcgaat ccggactcat ggacgcgaga
     9481 atacatgcgt ccactccata tatagttcgc agtgtgtaag ccctttcaaa atcagtaccg
     9541 ggatccaatg aaaccttccg ttctaatttc ccgtctcgcg gtgatgttcg ttgccggaat
     9601 ttcggcggtg tcttcgcaaa cgctggagtt gcaccaggcc gaccggcagc cattgcccgt
     9661 catcgatccg gcgtcggacg acgccacgct cgccatccag cgcttttcgt tgccgaaggg
     9721 catgcaggcg aagttgtggg ccgccgagcc catgctggcg aatccggtgg ccttcgattt
     9781 cgacgagaaa ggccggctgt tcgtctccga gacctaccgc tatcgcagca gcgtgctcga
     9841 catccgcgac tacatgggca tgctcgaact ggacctcgcg tcgcgcacca tcgaagaccg
     9901 caccgccctg catcgcaagg tgttcggcgc ccaggcgaag gatttcgcca tcgagggtga
     9961 agtggtgcgc ctggtcgaag acaccaacgg cgacggggtg gccgacaaga gccgcgcata
    10021 tgccgacggc ttcaacaccg aactcgacgg catcgcctcg ggcgtgctcg cgcgccatgg
    10081 caaggtgtgg ttcacgaaca tcccgagcct gtggttgtta gaggaaggca ccgacggcaa
    10141 gctgaagaag gacgagctgc tgcgcggttt cggcgtgcgc tacaactaca ccggccacga
    10201 ttttcacggc ctggcgatgg gccacgacgg caagatctat tactccatcg gcgaccgcgg
    10261 cacacacgtg aaaggcaagg aaggccaggt ggtcggctat ccggatgaag gcgcggtgtt
    10321 ccgttccaat cccgatggca ccgagttcga gatggtcgcg cgcggcctgc gcaatccgca
    10381 ggagctggtg ttcgacgaac acggcaatct cttcaccggc gacaacgaca gcgaccaggg
    10441 agacatggaa cgcctggtat atctcgtcga aggcggcgac agcggctggc gcatcggtta
    10501 tcagcacgcg ccgctcggca agggcgggcc ctggatgcgc gaaggcctgt ggaagccgga
    10561 gttcgcgggg cggcctgcct atctactgcc gcccgtctgc aacatcgagg acggcccgtc
    10621 cggcctcacc tattacccgg gcaccggtct cgaccccagg tacgccggac atttcttcat
    10681 cacgcacttc aagggcagca tcgcgcgcag cggcgtgcag acctacacgc tgaagcagaa
    10741 cggcgccacg ttcacaccca ccagcagcca gcaattcatg ggcggtgtgc tcccgacgga
    10801 cgtcacgttc gccccggacg gcaagctgta catcctcgac tgggtggacg gctggcccaa
    10861 gtcgaacaag ggccgcgtct atggaatcgc gccggcgaac ccggagccgg cgcaggcgaa
    10921 ggtcagtgcc gagctcacga agctgctgaa agatggattc acgcagcgta acgaccagga
    10981 gctggcggcg ttgttgtc
//
LOCUS       DASQJS010000015         9777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1580284, whole genome shotgun sequence.
ACCESSION   DASQJS010000015 DASQJS010000000
VERSION     DASQJS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9777
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580284"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /gene="rpoE"
                     /locus_tag="VEW08_00635"
     CDS             complement(<1..559)
                     /gene="rpoE"
                     /locus_tag="VEW08_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HYJ39257.1"
                     /translation="MTVEESDLVLVKRVQRGDKTAFDLLVRKYQHKVVKLVLRYVRNP
                     AEAEDIAQEAFIKAYRALPQFRGDSAFYTWMYRIAINTAKNSLASRDRSPIAYDLDLT
                     DPEESHSVQTKLQDPDTPEGMALTEEIRQIVNSAIEGLPEELKTAIVLRELDGLSYEE
                     IAAAMECPVGTVRSRIFRAREAIDKR"
     gene            complement(1125..1364)
                     /locus_tag="VEW08_00640"
     CDS             complement(1125..1364)
                     /locus_tag="VEW08_00640"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HYJ39258.1"
                     /translation="MDTELGKLQWQCRRGMKELDFLLVRYLRERYGQAASDERAAFAD
                     FLELPDPDIARYLLAGDVPSDPRQAELCRHLLLSN"
     gene            complement(1381..2169)
                     /locus_tag="VEW08_00645"
     CDS             complement(1381..2169)
                     /locus_tag="VEW08_00645"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HYJ39259.1"
                     /translation="MVEFTLPANSKIDKAAGKKYPAAAGAKRVRKFVIYRFDPDSGLN
                     PRMDTYEVDMDACGPMVLDALIKIKNEVDSTLTFRRSCREGICGSCAMNIGGLNTLAC
                     TKACDEVKGDVKIYPLPHMPVVKDLVPDLTNFYAQYAAVKPWLQTRTPAPPDRERPQS
                     KEDQEKIDRPSACILCACCSTSCPSYWWNSDRYLGPAALLAAYRWIIDSRDEATGERL
                     DELEDPFRLYRCHTIMNCTEACPKDLNPAKAIAEIKKMMLERQH"
     gene            complement(2183..3967)
                     /gene="sdhA"
                     /locus_tag="VEW08_00650"
     CDS             complement(2183..3967)
                     /gene="sdhA"
                     /locus_tag="VEW08_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HYJ39260.1"
                     /translation="MKDYEFIDHSYDVVVVGAGGAGLRATLGLAEAGLTTACITKVFP
                     TRSHTVAAQGGISAALGNMGEDDWRFHFYDTIKGSDWLGDQDAIEFMCKEAPAAVIEL
                     EHYGVPFSRTNDGRIYQRPFGGMTTHYGKGTAHRTCAAADRTGHAMLHTLYQQSIKHR
                     AEFFVEYFALDLIMQDGACRGVVALDLATGRLHRYRAHTVILATGGYGRAYFSCTSAH
                     TCTGDGGGMALRAGLPMQDMEFVQFHPTGIYGSGCLITEGSRGEGGYLTNADGERFME
                     RYAPNAKDLASRDVVSRSMTIEIRGGRGVGKDKDHIHLHLEHLGPEVIHARLPGIAET
                     ARIFAGVDVNKDPIPVLPTVHYNMGGIPCNVHGEVVTSKSGNPDTVVPGLMAVGEAAC
                     VSVHGANRLGSNSLLDLVVFGREAGRHAATIVKPGQPHSPLAKDAGDFAVARLDKLRN
                     AKGSRSTAEIRLEMQKIMQRDAAVFRTGDTLREGVAALSKTHASFADVKLTDRGLIWN
                     TDLVETIELDNLLGQAMATIVSAENRKESRGAHAREDFESRDDVNWQKHTLCWVDDAG
                     KTRIDYRPVHMNTLTADVEPIPPKARTY"
     gene            complement(3964..4341)
                     /gene="sdhD"
                     /locus_tag="VEW08_00655"
     CDS             complement(3964..4341)
                     /gene="sdhD"
                     /locus_tag="VEW08_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HYJ39261.1"
                     /translation="MSLRTPLSKVLGRGSAGEGVGHWWMQRVTAVALLPLTAWFVISL
                     LRSPLQSYDAMRDWLGQPWVAVLCSLLVFTLAWHSKLGVQVVIEDYVHGKGAKTTLLL
                     LSTFVHFAAAVAGIFAILRLTLS"
     gene            complement(4338..4739)
                     /gene="sdhC"
                     /locus_tag="VEW08_00660"
     CDS             complement(4338..4739)
                     /gene="sdhC"
                     /locus_tag="VEW08_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HYJ39262.1"
                     /translation="MDQRPLSPHLGVYKFAYTMSLSILHRITGCAAAVGFLFFVWWLM
                     ALASGPYTYASVMNALSSPFAKLLLAGFTFSFVFHFCNGIRHLIWDTGRGLERAQARR
                     SGAVVIVAALLLTALVIWCGCNAMEFAPVMQ"
     gene            4996..5862
                     /locus_tag="VEW08_00665"
     CDS             4996..5862
                     /locus_tag="VEW08_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39263.1"
                     /translation="MSTSLGVNLSSPPGGLGVLAFRGPDATRFLQGQLSAEVEKLGVG
                     ATTLAGLHSPQGRTVAILALLRSAAEEVLAVFPQELAGVVEQRLRKFILRAKVQIEDL
                     SENYRVIGIPAGADSAPEWAQLSWGDRRVLLVPTARAGEFSSEAGERSSWELADISAG
                     LPQVYTATSEAFVAQMLNLDLLGAIAFDKGCYTGQEVIARAHYRGRVKRRLQRWHAAA
                     GTPPLAGDSARTGDGRALTVVRAAKSQDGGWDLLAIGTFAAGVAPESTAPTQPDALTV
                     VGPLPLPYPLPD"
     gene            complement(5873..6745)
                     /locus_tag="VEW08_00670"
     CDS             complement(5873..6745)
                     /locus_tag="VEW08_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ39264.1"
                     /translation="MRERSGVAHDRAVMPMRYGFADTLSGQLHFRACGAGPDVVLLPW
                     LPASGRMFEHVMPHLARSGFRAIAFDLAGSGRSHKNCRNWTVQQYATDVLEACGSLHT
                     VPYTVVGGRFSASVAVEMLLASPAQVTGAVLDGVPGLAPEELRKMTGPTTGLSPRLSD
                     DGAHRSFAFDVSVRTLKDWDPSFELTAESLPQVYELMRDYLELGFESIAAGVEADARG
                     GKSTHDVLARLGEVSQRVLVMTSDADTLAPAYNRALERARNSRGHKFAGNHPVLTPAR
                     AAEYAQVVVSFARR"
     gene            complement(6820..7620)
                     /gene="murI"
                     /locus_tag="VEW08_00675"
     CDS             complement(6820..7620)
                     /gene="murI"
                     /locus_tag="VEW08_00675"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HYJ39265.1"
                     /translation="MNAPIGVFDSGVGGLTVLRALIGELPNEDFLYLGDTARLPYGTK
                     SPETVARYSVRAAEALESRGIKALVVACNTASATALPSLRERFPSLPVIGVIEPGAQA
                     ACEASVSGRIAVLATEGTVRGGAYERAILALRPGAHVTQVSAQVFVALAEEGWSEGEA
                     VDAVTERYLAHLDARVDTVVLGCTHFPLLAGAISRQLGPTRRVVDSAATTARATALEL
                     AVRKLAAPAARTGAVHLLATDSPERFARVGARFLGSSIAERSVQAIDL"
     gene            complement(7617..9143)
                     /gene="lysS"
                     /locus_tag="VEW08_00680"
     CDS             complement(7617..9143)
                     /gene="lysS"
                     /locus_tag="VEW08_00680"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HYJ39266.1"
                     /translation="MSDDPSKPQEGQIVAERRAKLTALREQAKAAGAPAFPNDYRRDV
                     LATHLASEYGEKPAEWFDSNPPIRLHVAGRMMFQRIMGKASFAKLQDRTGQIQIFLQR
                     DALGESYEAFKKYDVGDILAATGTLFKTKTGELSVRVEQLRMLTKSLRPLPDKWHGMA
                     DVDTRYRQRYVDLIVNEQSRNVFITRTRILKYLRDFLDSMDFLEVETPMLQSIPGGAA
                     ARPFRTHHNALDMDMYLRIAPELFLKRLTVGGFERVYEINRNFRNEGVSTRHNPEFTM
                     LELYVAYADYKDLIVMVERAMQGVADVVFGKRQFEYQGRAYDLDKPFTRLTVLEAVAA
                     HTPGFDVSRARDVAYLRELCAASGIKFRPGDGAGKLQIELFEKFAEHTFMDPTFVYAY
                     PTEVSPLSRANDADPFLTDRFEFFVAGRELANGFSELNDAEDQAARFRAQVERKDAGD
                     DEAMYYDADYVRALEYGMPPTAGLGVGIDRLVMFFTDAPSIRDVLLFPHMRPENLQQQ
                     "
     gene            complement(9140..>9777)
                     /locus_tag="VEW08_00685"
     CDS             complement(9140..>9777)
                     /locus_tag="VEW08_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HYJ39267.1"
                     /translation="WAASRGFDAEVIDSQAGEVAGMKSATIEIKGDYAYGWLRTETGV
                     HRLVRKSPFDSGNRRHTSFASVFISPEVDDDIKVDLNPADIKMDVYRSSGAGGQHVNK
                     TESAVRLTHEPTGLVVACQNERSQHKNRATAMKMLKAKLYELEFNKRNAAAKVLEDSK
                     SDVSWGNQIRSYVLDQSRIKDLRTGVEIGDTNKVLDGNLDPFMEASLKAGL"
BASE COUNT         1765 a         3034 c         3177 g         1801 t
ORIGIN      
        1 ggcgcttgtc tatggcctcg cgcgctcgaa aaattcgtga acgaaccgtg cccacgggac
       61 attccatggc ggcggcgatt tcctcgtagc tcaatccatc cagctcgcgc agcacgatgg
      121 ccgtcttgag ttcttcaggc aatccttcga tcgcggagtt cacgatctgg cgaatttctt
      181 cggtgagcgc catgccttcc ggggtgtcgg ggtcctgaag cttggtttgc acgctgtgcg
      241 attcctcagg gtcggtcagg tcgagatcgt aggcgatggg gctgcggtcc cgcgacgcga
      301 gactgttctt ggcggtattg atagcgatgc gatacatcca ggtgtagaag gcgctgtcgc
      361 cacgaaactg aggtaaggcc cgatacgctt tgatgaaagc ttcctgcgca atgtcttccg
      421 cttcggccgg attgcgaacg tatcgcaaca ccagcttcac cactttgtgc tggtacttgc
      481 gcaccagcag atcgaacgcg gttttgtcac cgcgctgaac gcgtttgacg agaaccaggt
      541 cactttcttc aaccgtcaca ggagcacccc ggctcctttc gagctgaata gaccctgaac
      601 gcccacgaaa gtttcctttt ggggcgcagc taatcgaatg atttttaatg gcgcgtgacg
      661 cacttcaaac cttgggccgg atggtaacag cggggcgccg ggagcgcccg gaacccctgc
      721 gcaggtgccc gttgagacac cggctgaggc ggcggaaggc aagcggcgca ccggccgcct
      781 cctccaccag cacggatcgc gttcccactg gcgttgcgaa ccgcaatacc cagaatcgca
      841 ggcccaaccg gaacgatccg tttgcgaggg tggcggggtg tggcgcgagt gttgctccaa
      901 ggcaaacggt cagttcgccc gcttcgttcc attcgatcgc ccgaactgcg cgcggcccga
      961 tcagaagtgc gaatgcgcgc agcccgcaaa cgcccgccgt ggcgacggcg atgcaaatgg
     1021 tgaaacgcgc aggccacggc agtaccacgg cgaaccacgt gaccatgcag gccagcacca
     1081 gccacgtgat ccaggcgctc accgcgaaca gggatggaat caattcaatt cgatagcagg
     1141 aggtgccggc acaattctgc ctggcgggga tcggatggga catctcccgc aagcagataa
     1201 cgcgcgatgt ccgggtcggg caattcgaga aagtccgcga aggcagcgcg ttcgtcgctt
     1261 gcggcctgac cgtagcgttc tcgcagatag cgaacgagca ggaaatcgag ttccttcatg
     1321 ccgcggcggc actgccattg aagcttgccg agctcggtgt ccacgggccg cgcgtccaaa
     1381 tcagtgctgg cgctcgagca tcattttttt gatttcggcg atggcttttg ccggattgag
     1441 atccttcggg caggcctcgg tgcaattcat gatggtgtgg cagcgataga gccggaatgg
     1501 atcttccagt tcatcgaggc gctcgccggt cgcctcgtcg cgtgaatcga tgatccagcg
     1561 ataggccgcg agcagcgccg cagggccgag gtagcggtcg ctgttccacc agtagctggg
     1621 acaggatgtg gagcagcacg cgcacaggat gcaggccgag gggcgatcga tcttttcctg
     1681 atcttccttc gactggggac gctcgcgatc tggcggcgcc ggcgtgcggg tctgcagcca
     1741 aggcttcacg gcagcgtatt gcgcatagaa attggtgaga tcgggcacca ggtctttcac
     1801 caccggcatg tgcggcagtg gatagatctt cacgtcgcct ttcacctcgt cacacgcctt
     1861 ggtgcaggcg agtgtgttga gtccgccgat gttcatcgcg caactgccgc agattccctc
     1921 gcggcacgag cggcgaaacg tcagcgtgga atcgacctcg ttcttgatct tgatcagcgc
     1981 gtcgagaacc atcgggccac aggcgtccat gtccacttca taagtgtcca tgcgcgggtt
     2041 caggccggaa tccggatcga accggtagat gacgaacttg cggacgcgct tcgcaccggc
     2101 cgccgccgga tatttctttc cggccgcctt gtcgatcttc gagttcgcgg gaagcgtgaa
     2161 ttcaaccatc ccaaaagatc ctttagtacg ttcttgcctt gggcggaatc ggctcgacat
     2221 cggcagtgag cgtgttcatg tgcaccggac ggtagtcgat gcgcgtcttg cccgcgtcgt
     2281 cgacccagca gagcgtgtgt ttctgccagt tcacgtcatc gcggctctcg aaatcctcgc
     2341 gcgcatgcgc gccgcggctt tccttgcggt tctcggcgct gacgatggtc gccatggcct
     2401 gccccaacag gttgtcgagc tcgatggttt cgacgagatc ggtgttccag atcagcccgc
     2461 ggtcggtcaa cttgacgtcg gcgaaggaag catgcgtctt cgacagcgcc gcgacacctt
     2521 ccctgagcgt gtcaccggtg cggaacacgg cggcgtcgcg ctgcatgatc ttctgcatct
     2581 cgagccggat ctcggcggtg ctgcgcgaac ctttcgcatt gcgcagttta tcgagccggg
     2641 caacggcgaa atccccggca tccttcgcga gcggcgaatg cggctgaccg ggcttcacga
     2701 tggttgccgc gtggcgcccg gcctcgcggc cgaacacgac gagatcgagc agcgagttgc
     2761 tgccgagccg gttcgcgccg tgaacggaca cacaggcggc ttcgcccacg gccatgagtc
     2821 ccggaaccac ggtgtcggga ttgccggact tcgacgtgac cacttcacca tgcacgttgc
     2881 agggaattcc gcccatgttg tagtgcaccg tgggcaacac ggggatcgga tccttgttca
     2941 cgtcgacgcc cgcgaaaatg cgcgcggtct cggcgatgcc cggcaggcgc gcgtggatca
     3001 cctcgggccc gaggtgttcg aggtgcaggt gaatgtgatc cttgtccttg cccacgccgc
     3061 ggccgccgcg aatttcgatg gtcatcgagc gcgacaccac gtcgcgcgaa gcgaggtctt
     3121 tcgcattggg cgcgtaacgc tccatgaagc gttcgccgtc ggcgttggtg agatatccgc
     3181 cctcgccgcg cgaaccttcg gtgatcagac atcccgaacc gtagatgccg gtgggatgaa
     3241 attgaacgaa ctccatgtcc tgcatcggca gaccggcgcg caacgccatg ccgccgccgt
     3301 cacccgtgca cgtgtgcgcc gaggtgcacg agaaataggc gcggccgtag ccgcccgtgg
     3361 cgaggatcac ggtgtgcgcg cgataacgat gtaacctgcc cgtggccagg tcgagcgcca
     3421 cgacaccgcg gcaggcaccg tcctgcatga tgagatcgag cgcgaaatat tcgacgaaga
     3481 attcggcgcg atgcttgatc gactgctggt acagcgtgtg cagcatggcg tgaccggtgc
     3541 gatcggccgc cgcgcaggtg cgatgcgccg tgcctttgcc gtagtgcgtg gtcatgccgc
     3601 cgaagggccg ctggtagatg cggccatcgt tggtgcgcga gaacggcacg ccgtagtgct
     3661 cgagctcgat gaccgcggcc ggcgcttcct tgcacatgaa ctcgatggcg tcctggtcgc
     3721 cgagccagtc cgaacccttg atggtgtcgt agaaatggaa acgccagtcg tcctcgccca
     3781 tgttgccgag cgctgccgag atgccaccct gcgccgccac cgtgtggctg cgagtcggga
     3841 acaccttggt gatgcaggcg gtagtcaggc cggcttccgc caggcccagc gtggcgcgca
     3901 gtcccgctcc gccggcgccg accacgacga cgtcgtagga atgatcgatg aattcgtagt
     3961 ctttcatgaa agcgtcaggc gaaggatcgc gaagatgccg gcgaccgcag cggcgaagtg
     4021 gacgaacgtg gagagcagca gcagcgtcgt tttggcgccc ttgccatgga cgtagtcttc
     4081 gatgaccacc tggacaccga gcttggaatg ccaggcgagc gtgaacacca gcagcgagca
     4141 caagacggcg acccagggct ggcccagcca gtcgcgcatc gcgtcgtagg actgtagcgg
     4201 cgaccgcagc agcgagatga cgaaccaggc ggtaagcggc agcagcgcca cggccgtgac
     4261 ccgctgcatc caccaatgtc ccacaccctc gccggccgaa cctcgcccga gtactttcga
     4321 caagggagtg cggaggctca ttgcatcacc ggggcgaatt ccatggcgtt gcagccgcac
     4381 cagatgacca atgccgtgag caacagcgcc gcgacgatga ccaccgcgcc gctgcggcgc
     4441 gcctgcgcgc gctctaggcc gcggccggta tcccaaatga ggtgtcgaat gccgttgcag
     4501 aagtgaaaga cgaatgaaaa ggtgaagccc gccagcagta gtttggcgaa cggcgaactc
     4561 agggcgttca taacactcgc gtaggtgtac gggcccgatg ccagagccat gagccaccag
     4621 acgaagaaca ggaaacccac cgccgctgcg caaccggtta ttcggtgcag gatcgacagc
     4681 gacatggtgt acgcgaactt gtacacgccg agatgagggg aaagcggtcg ctggtccatc
     4741 tttaaacctt agaaatcgac gcagcggcca ttcttttccc agtcgccgaa gcgagtgggt
     4801 tcaggaccct gcgggccgcc gatttcagcg ggcactgctt tcccggtgga ctcgacggcg
     4861 cctgcgcctg gcacggcagc cgttttggcg gcattggcgc tcgaggaatc tagctcggaa
     4921 ggggcattcg gcacgggccc gaattctgcc agaatcagcc gcgtccgacc atatgaaacc
     4981 cctgcaaaaa caaagatgag cacttctctc ggcgtcaatc tgtccagccc acccggagga
     5041 ttgggcgtgc tggcgtttcg cggacccgat gccacacgct ttcttcaggg tcagctttcg
     5101 gccgaggtgg aaaagctcgg cgtcggtgca accacactcg ccggcctgca cagcccgcag
     5161 ggtcgcaccg tggcgatact cgcgttgttg cgatcggctg cagaagaagt gttggcggtg
     5221 tttccgcaag agttggcggg cgtggtggaa cagcggctgc ggaaattcat cttgcgcgcc
     5281 aaggttcaaa ttgaagacct gtcggagaac tatcgggtca tcggcatccc ggccggcgcg
     5341 gattccgcac cggaatgggc gcagttgtcc tggggcgatc gacgcgtgct gctcgtgccc
     5401 acggcacgag cgggcgaatt ctcgagtgaa gcgggtgaac gctcgagctg ggagcttgcc
     5461 gacatctcgg ctggcctgcc gcaggtctac acggcgacca gcgaagcctt cgtggcgcag
     5521 atgctgaacc tcgacttgtt aggggccatc gccttcgaca aggggtgtta cacgggccag
     5581 gaagtcattg cgcgcgccca ttatcgcggc cgcgtgaaac ggcgcctgca gcgctggcac
     5641 gcggcggccg gcacgccgcc tctcgcggga gacagcgccc gaacgggcga tggacgcgca
     5701 cttacggttg tgcgtgcagc gaaaagccag gatggcggat gggatctgct ggcgattggc
     5761 accttcgcgg cgggcgtagc gcctgaatcg accgcaccca cgcagcccga tgcgttaact
     5821 gtggttggcc cgctgccgct gccgtatcca ctgccggatt aggccgccct gactaacgcc
     5881 gcgcgaacga tacgaccacc tgtgcgtatt cggcggcgcg cgccggcgtc aacaccgggt
     5941 ggttgccggc gaacttgtgg ccgcgcgagt tgcgcgcccg ctcgagcgcg cggttgtagg
     6001 cgggtgcgag cgtgtccgcg tccgatgtca tgaccagcac gcgctgcgac acttctccga
     6061 ggcgcgccag cacgtcgtga gtgctcttgc cgcccctggc atcggcctcg acgccggctg
     6121 cgatgctctc gaatccgagt tcgaggtagt cgcgcatgag ttcgtagacc tgcggcagag
     6181 actccgccgt caactcgaat gacgggtccc agtccttcag tgtgcgcacg ctgacgtcga
     6241 acgcgaagct gcgatgcgcg ccgtcgtccg agagcctggg cgagaggccc gtggtgggcc
     6301 cggtcatctt gcgaagttct tcgggggcga gccccgggac gccatcgagc acggcgccgg
     6361 tgacctgcgc gggtgaggct aacaacatct ccacggcgac cgaggcggag aaccggccac
     6421 cgactacggt gtacggcacg gtatgcagcg agccgcaggc ctcgagcacg tccgtcgcgt
     6481 attgctgcac agtccagttc cggcaattct tgtgcgaccg gcctgagccc gcgagatcga
     6541 aagcgatggc gcgaaacccg gaacgcgcga ggtgcggcat gacgtgttcg aacatgcggc
     6601 cgctggctgg aagccagggc agcagcacta cgtcggggcc tgcgccgcag gcgcggaagt
     6661 gcaattgacc cgacaacgta tccgcgaagc cataacgcat cggcatcacg gcgcgatcgt
     6721 gcgcaactcc tgatctctct ctcatccagt ccttcccttg atgtcgtccg caggcccgtg
     6781 aatcgggggc gcccagtgta accgcgttat ggaaagtatc taaagatcga ttgcctgaac
     6841 cgacctttcg gcgatcgatg atcccaaaaa tcgcgcgccg acgcgggcga aacgctcggg
     6901 gctgtcggtg gccagcaggt gaacggcgcc ggtgcgcgcg gccggcgccg caagctttcg
     6961 caccgcgagt tcgagcgccg tggcccttgc ggtggtcgcc gccgaatcca cgacgcggcg
     7021 cgtcggaccc aattgccggg agatcgcgcc cgcgagcagc ggaaaatgtg tacagccgag
     7081 cacgacggtg tcgacccgcg catcgagatg ggcaaggtag cgctcggtca cggcatcgac
     7141 ggcttcgcct tcgctccaac cctcttcggc cagcgccacg aacacttgcg cggaaacctg
     7201 ggtgacatgc gccccgggac gcaaggcgag gatcgcgcgc tcatacgcgc cgccgcggac
     7261 tgtgccttcg gttgcgagca cggcgatgcg cccgctgacg gaagcttcac aggccgcctg
     7321 cgcaccgggc tcgatcacgc cgatcacggg cagcgacggg aaacgctcgc gcaggctggg
     7381 caatgccgtg gcggatgccg tgttgcaggc cacgaccagc gccttgatgc cgcggctttc
     7441 cagcgcctcg gcagcccgta ccgaataacg cgccacggtt tccggactct tggtgccgta
     7501 gggaaggcgc gcggtatcgc cgagatagag aaagtcctcg ttcggaagct cgccgatcag
     7561 cgcccgcagc accgtgagtc cgccgactcc ggagtcgaac actccaatgg gcgcattcat
     7621 tgctgctgaa gattctcggg acgcatgtgc gggaacagca gcacatcgcg gatggacggc
     7681 gcatcggtga agaacatgac cagccggtcg atgcccacac ccagacccgc ggtcggcggc
     7741 atgccgtatt ccagcgcgcg cacgtagtcg gcgtcgtaat acatggcctc gtcgtcaccc
     7801 gcatccttgc gctcgacctg tgcgcggaaa cgcgccgcct gatcctcggc gtcgttgagc
     7861 tcggagaatc cattggcgag ctcgcggccg gcaacgaaaa actcgaagcg atcggtgagg
     7921 aacgggtccg catcgttggc gcgcgacagc gggctgacct cggtgggata ggcgtacacg
     7981 aacgtggggt ccatgaacgt gtgttcggcg aatttttcga agagctcgat ctgaagtttg
     8041 cccgcgccgt cgcccggcct gaacttgatg ccgcttgcag cgcacaactc gcgtagatag
     8101 gcaacgtcgc gcgcgcggga cacatcgaag cctggcgtgt gggcggccac ggcctcgagc
     8161 acggtgaggc gcgtgaacgg cttgtcgagg tcgtacgccc tgccctggta ttcaaattga
     8221 cgcttgccga atacgacgtc cgctacgccc tgcatcgccc gttccaccat gacgatgaga
     8281 tctttgtagt cggcatacgc gacgtacagt tcgagcatcg tgaactcggg gttgtgccgc
     8341 gtggacacgc cctcgttgcg gaaattgcga tttatctcgt acacccgctc gaagccgccg
     8401 accgtgagac gcttgaggaa gagctccggc gcgatgcgca ggtacatgtc catgtcgagc
     8461 gcgttgtgat gtgtgcgaaa cgggcgcgcg gcagcgccac ccggaatcga ctgcaacatc
     8521 ggcgtctcga cttcgaggaa atccatggaa tcgaggaaat cgcgcaggta cttcaggatc
     8581 cgcgtgcggg tgatgaatac gttgcggctc tgctcgttga cgatgagatc gacgtaacgc
     8641 tggcgataac gcgtgtcgac gtcggccatg ccgtgccact tgtcgggcag cggccgcaac
     8701 gacttggtca gcatgcgcag ttgttcaacc cttaccgaca gctcgccggt cttcgtcttg
     8761 aacagcgttc cggtggcggc gaggatgtcg ccgacgtcgt acttcttgaa tgcttcgtac
     8821 gattcgccga gtgcatcgcg ctgcaggaag atctggatct ggccggtgcg gtcctggagc
     8881 ttggcgaaac tcgccttgcc catgatgcgc tggaacatca tgcggcccgc gacgtgcagc
     8941 cggatcggcg gattcgagtc gaaccactcc gccggctttt cgccgtactc ggatgccagg
     9001 tgtgtcgcga gtacgtcgcg gcgatagtcg ttcgggaaag cgggcgcgcc ggcagccttc
     9061 gcttgttcgc gcaacgccgt gagctttgca cgccgctcgg cgactatctg gccttcctgg
     9121 ggtttacttg gatcgtcgct cacaggcccg ccttcagtga agcttccatg aaaggatcga
     9181 gattgccgtc gagcaccttg ttggtgtcgc cgatctcgac gccggtgcgc agatccttga
     9241 tgcgcgactg gtcgagtacg tacgaacgga tctggttgcc ccagctcacg tcgctcttgg
     9301 agtcttccag aactttcgcc gctgcattgc gtttattgaa ttccagctcg tagagcttcg
     9361 ccttcagcat cttcatggcc gtggcgcggt tcttgtgctg gctgcgttcg ttctggcagg
     9421 cgacgaccag ccccgtgggc tcgtgcgtga ggcgcaccgc cgattcggtc ttgttgacgt
     9481 gctggccgcc ggcgccgctc gaacgataga cgtccatctt gatgtcggcc gggttcaggt
     9541 cgaccttgat gtcgtcgtcg acctcgggcg atatgaacac cgaggcgaac gaggtgtggc
     9601 ggcgattgcc ggaatcgaaa ggcgacttgc gcaccagccg gtgcacgccc gtctcggtac
     9661 gcagccagcc gtacgcatag tcacccttga tctcgatggt ggcgctcttc atgcccgcga
     9721 cttcaccggc ctgtgagtcg atgacttcgg catcgaagcc gcgcgacgcc gcccact
//
LOCUS       DASQJS010000016         4916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1266070, whole genome shotgun sequence.
ACCESSION   DASQJS010000016 DASQJS010000000
VERSION     DASQJS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266070"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1102)
                     /locus_tag="VEW08_00690"
     CDS             complement(143..1102)
                     /locus_tag="VEW08_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ39268.1"
                     /translation="MSSHRRDLAFAALTLALFIAVTIAFPGFAAPRNLAGLLDDTAIL
                     IMLALGQMLVILVRGIDLSIAANLALCGMLAAVFDRAWPGAGIAPVLALTLVSGALLG
                     AFNGLLVWKLRLPPIVVTLGTMSIYRGAIYLLSKGAWVNENEMSRGFLAFPRIEFLGL
                     TLLSWLAVVIAVAFVIALRRSRVARDLYAAGGNPEAANYSGIDAGRMQFIAYTICGAI
                     AGVCGYLWVARFAVAYTDIALGFELSVIAACLIGGVAIAGGVGSAVGVVLGCLFIGII
                     RSSLPLVGISAFWQMFINGVVILVAVLLSARQREYKRTILEAA"
     gene            complement(1099..2541)
                     /locus_tag="VEW08_00695"
     CDS             complement(1099..2541)
                     /locus_tag="VEW08_00695"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HYJ39269.1"
                     /translation="MRSSLLTLTDIRKSFGGVRALERVSLELPRGAVTALIGENGAGK
                     STLVKILSGVYQPDGGVIHFDGAAVRIADPQAARALGIGVVHQECLAFDNLSVAENLF
                     VNCQPARRGLVNWASMRAQATAVLRDLDADFDADLPAGRLSIAQKHVMQIARALAHES
                     RVLIFDEPTASLSQRECVELFRIVHKLRDAGCALLFISHKFEEIFALADRYAVFRDGV
                     SVSAGNVGEVTGDELIRLMVGRPVDQLFPKIETTPGAELLRVDGLSRAGEFEDISFAL
                     RRGEILGIYGLVGAGRTELAQVLFGINAPDSGSITSGADIALVPEDRQRQGSILPFSV
                     AANIALPNLATLAPRGWCRSQDEAQLARHWIEKLAIRAQGPEQPVHNLSGGNQQKVVI
                     AKWLARRPGVLILDEPTKGIDVGAKAAVHAVTSEFARDGGGVLMISSDLPEILGMSDR
                     VLVMRRGRLRGEFARGQATSEILLRAASDA"
     gene            complement(2557..3555)
                     /gene="rhaS"
                     /locus_tag="VEW08_00700"
     CDS             complement(2557..3555)
                     /gene="rhaS"
                     /locus_tag="VEW08_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HYJ39270.1"
                     /translation="MLRRTLLACCLLAVTLSGCGDRKVEPEKPARTRIGVVAKSLGNG
                     FFDAVHKGADEAARELDAEIIFNGPTTPTAEGQIEVLNSLIAQRVDAIAVSANDPDAL
                     VPTLKRAMERGIKVVSYDSGVAAAGRIAHLAPSSDQLIGETVVALAAELAPGGKGKFA
                     VVSATPTSTNQNSWLAEMRKVLSQHPGLELVSVVYGDDVSDKSYREAVAVLKQHPDLS
                     VLVSISSVGIVASARAVEDQGLRGKVPVTGLGLPSELVGYVQKGVVPKFAIWNPIDLG
                     YATTQIAVRLARGDDPAKPVYMGRMGEVTFGPDGVGAMSKPLIYDINNVADFAKIF"
     gene            complement(3576..4379)
                     /locus_tag="VEW08_00705"
     CDS             complement(3576..4379)
                     /locus_tag="VEW08_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HYJ39271.1"
                     /translation="MLEKERHNLILKLVDERSIVSVGDLLDLLGASEATIRRDINALA
                     EKGEVKRIRGGAEAVRPRHQPHLVGMPFAMSQDVSVPQKRAIARAAAELIVPGESIII
                     NGGTTTFALVEFLENSDLDILTNSFPIAVKLFATSRNRITLPGGTIFREQNIVLSPFE
                     NDTIESFWGNKLFVGCYGLNRFGMMEADPLIVQAQTKLLKRSEHVIVMADSSKLRRKS
                     AMIVAGLDRINTIVTDDGAKDEELEDFRSAGIKVIVAKVTEEDDLKKLA"
     gene            4517..>4916
                     /locus_tag="VEW08_00710"
     CDS             4517..>4916
                     /locus_tag="VEW08_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39272.1"
                     /translation="MTVVQMAPQASLEIENRWDDTYAARLSPAELLLYRSNLLGSDKR
                     ITNFGGGNTSAKLKEKDPLTGQEVEVLWVKGSGGDLGTMKLDGLSTLYMEKFLGLEKF
                     YGSSKAAGKDPDGTLVPLYAHSAFNLNPRAA"
BASE COUNT          953 a         1570 c         1530 g          863 t
ORIGIN      
        1 ttgcgcgctc ggtgaagttg aacgtcgcat ccgacagcgt gtcgacgctc aggaaatacg
       61 gtgagatggt cgagaacacg atgaattcga gcaccagtac cagaaccagc atcgattccc
      121 atcgaaggag gttcgtcctc atttacgcag cctcgaggat ggtgcgcttg tactcgcgct
      181 gccgggcgct caacagcacg gcaaccagga tgaccacacc attgatgaac atctgccaga
      241 aggccgagat gcccaccagc ggcaggctgc tgcgaatgat gccgatgaac aggcacccca
      301 gcaccacgcc caccgccgag ccgacgccac cggcaatcgc cacaccgccg atgaggcacg
      361 cagcgatgac cgagagctcg aaaccgaggg cgatgtcggt gtacgccacc gcgaagcgcg
      421 ccacccacag ataaccgcag acgccggcga tggcgccgca gatcgtgtat gcgatgaact
      481 gcatgcgtcc cgcatcgatg ccggagtagt ttgccgcctc gggattgccg ccggccgcgt
      541 acaggtcgcg cgcgacacgt gagcggcgca gtgcgatcac gaaggccacg gcgatgacga
      601 ccgccagcca cgagagcagc gtgagcccca ggaattcgat gcgcggaaaa gccaggaaac
      661 cgcgcgacat ttcgttctcg ttgacccagg cgcccttgga caggagatag atcgcaccac
      721 ggtagatgga catggtgccc agcgtcacca cgatgggcgg cagccgcagt ttccagacca
      781 gcaggccatt gaaggcgcct aacaaggcac cggaaaccag cgtaagcgcg agcacgggag
      841 cgatgccggc tcccggccat gcacggtcga aaaccgcggc cagcatgccg cacagggcga
      901 ggttcgccgc gatcgacaag tcgatgccgc gaacgaggat gaccagcatc tggcccagcg
      961 ccagcattat caggattgcg gtgtcatcga gcagtccggc cagattgcgc ggcgccgcga
     1021 agcccgggaa cgcgatggtc accgcgatga acagcgccag cgtgagcgcc gcaaacgcca
     1081 gatcgcggcg atgcgaactc atgcgtcact cgcggcgcgc agcaggattt cactggtcgc
     1141 ctgcccgcgc gcgaattcgc cgcgcaggcg gcctcgccgc atcaccagca cgcgatccga
     1201 catgccgagg atttccggca gatcggagga aatcatcagc acaccgccgc catcgcgcgc
     1261 gaactcgctg gtcacggcat gcaccgccgc cttcgctccc acgtcgatgc ccttggtggg
     1321 ctcatcgaga atcagaacgc cgggacggcg cgcgagccac ttggcgatca ccactttctg
     1381 ctggttgccg cccgagaggt tgtgcacggg ctgctccgga ccttgcgccc tgatcgcgag
     1441 cttctctatc cagtgtcgcg cgagttgggc ttcatcctgc gaacggcacc agccgcgcgg
     1501 tgccagcgtc gcaagattcg gcagggcgat attcgccgcg accgagaaag gcaggatgct
     1561 tccctgtctc tggcgatctt cagggacgag cgcgatgtcc gcgcccgaag tgatgctgcc
     1621 cgaatcgggc gcgttgatgc cgaacaatac ctgcgccagt tcggttctgc cggcgccgac
     1681 gagaccgtag atgccgagaa tctcgcccct gcgcaatgcg aacgagatgt cttcgaactc
     1741 accggcgcgg ctcaagccgt cgacgcgcag cagttcggca ccgggcgtgg tttctatttt
     1801 tggaaacagc tgatcgaccg gccggccgac catgagccgg atgagctcat cgccggtcac
     1861 ttcaccgacg ttgccggcac tcaccgaaac accgtcgcgg aacacggcgt agcgatccgc
     1921 gagcgcgaag atctcctcga acttgtggct gatgaacagc agcgcgcaac ctgcgtcgcg
     1981 cagcttgtgc acgatgcgga acagctcgac gcactcgcgc tgcgacagcg atgccgtggg
     2041 ctcatcgaaa atgaggacgc gggattcgtg ggccagcgcg cgcgcaatct gcatcacgtg
     2101 cttctgcgcg atgctcagcc ggcctgccgg caggtcggca tcgaagtccg cgtcgagatc
     2161 gcgcagcacg gccgttgcct gcgcgcgcat cgatgcccag ttcacgagcc cgcggcgggc
     2221 cggctggcaa ttcacgaaca ggttttccgc cacggacaga ttgtcgaagg ccaggcactc
     2281 ctggtgaacc acgccgatgc ccaaggcgcg cgcagcctgc gggtccgcga tgcgcacggc
     2341 ggcgccgtcg aaatggatga caccgccgtc gggttgatag acacccgaca gtattttcac
     2401 cagcgtggac tttccggcgc cgttctcacc aatgagagcc gtaaccgcgc cgcgcgggag
     2461 ctcgaggctc acgcgctcga gcgcgcgcac gccgccgaat gacttgcgga tgtcggtcag
     2521 cgtcagtaag gaagagcgca cggaatcggc gggccgtcag aagatcttcg cgaaatccgc
     2581 gacgttgttg atgtcataga tcaacggttt gctcatcgcc ccgacaccgt cgggcccgaa
     2641 tgtgacctca cccatgcgtc ccatatagac cggcttcgcg ggatcgtcgc cgcgggcgag
     2701 ccgcaccgcg atctgcgtgg tggcataacc aagatcgatc ggattccaga tcgcgaattt
     2761 cggcacgacg cctttctgca catagccgac gagttcggat ggcaggccga ggcctgtgac
     2821 cggcaccttg cctcggaggc cctggtcttc aacggcgcgt gccgacgcca cgatgcctac
     2881 cgagctgatg ctcacgagca ccgacaggtc cgggtgctgc ttgaggaccg cgaccgcttc
     2941 ccgatacgac ttgtcggaca cgtcgtctcc gtacacgacg ctcacgagct cgagcccggg
     3001 gtgttgcgac agtactttgc gcatctccgc gagccaggaa ttctgattgg tcgaagtggg
     3061 cgtggccgac accacggcga acttgccctt gccgccaggc gccaattccg cggcgagcgc
     3121 caccacggtc tcgccgatga gctgatccga ggatggcgca agatgcgcga ttcgccccgc
     3181 ggccgcgact cccgaatcgt atgaaaccac tttgatgcca cgctccatcg cacgcttgag
     3241 ggtgggcacc agcgcatcgg gatcgttcgc cgacaccgcg atggcgtcca cgcgttgcgc
     3301 gatcagcgaa ttgagcactt cgatctgacc ctccgcggtc ggggtcgtcg gtccattgaa
     3361 aatgatttcg gcgtcgagct cgcgcgcggc ttcgtcggcg cccttgtgaa cggcatcgaa
     3421 gaagccattg cccaatgatt tcgccaccac gccgatgcgc gtgcgggccg gcttttcagg
     3481 ctcgaccttc ctgtcgccac agccggacag cgtgacggcc agaaggcagc acgcaagcag
     3541 agttctgcgt aacaaagtat cccccgtgcc cgcgctcagg cgagcttttt cagatcgtct
     3601 tcttcagtga ctttcgcgac gatgaccttg atgccggcgc tgcggaaatc ctcgagctct
     3661 tcgtccttcg caccatcgtc ggtgacgatg gtgttgatgc gatcgagtcc ggccacgatc
     3721 atcgccgact tgcggcgcag cttggagctg tccgccatca cgatcacgtg ctcgctgcgc
     3781 ttcagtagtt tggtctgcgc ctgcacgatg agcggatcgg cttccatcat gccgaagcga
     3841 ttcagcccgt agcagccgac gaaaagcttg ttgccccaaa aactctcgat ggtgtcgttc
     3901 tcgaacgggc tgagcacgat gttctgttcg cggaagatgg tgccgcccgg cagcgtgatg
     3961 cggttgcggc tggtcgcgaa cagtttgacg gcgatcggaa aggagttggt gaggatgtcg
     4021 agatccgaat tctcgaggaa ttccaccagc gcaaaagtgg tcgtgccgcc gttgatgatg
     4081 atgctttcgc cgggaacgat gagctcggcc gcggcacgcg cgatcgcccg tttctgcggc
     4141 acgctcacgt cctggctcat ggcgaacggc atgcccacca gatgcggctg atgccggggg
     4201 cgcaccgcct cggcgccgcc gcgaattcgc tttacctcgc ccttctcggc aagggcgttt
     4261 atgtcgcgcc gaatggtggc ttcggacgcc ccgagaagat cgagcaggtc tccgacgctc
     4321 acgatggaac gctcatcgac gagcttcaaa atcaggttgt ggcgttcttt ttccagcatg
     4381 gggtcaccct cgacctaggt cgtgatcgat tatgaacgtg attgattgta tatgacggct
     4441 tcgtgtgtta aacagcctca ccgtaaaacg aggccgagcg tgcgtacttt agcgccgttc
     4501 agaccggaga cattggatga ctgtggttca gatggcgccc caggcgtctc tcgaaatcga
     4561 aaatcgttgg gatgacacct atgcagcccg gctctcaccc gccgagctgc tgctttaccg
     4621 ttccaatctg ctgggcagcg acaagcgcat caccaatttc ggcggcggaa acacctcggc
     4681 gaagctcaag gaaaaagacc ccctcaccgg ccaggaggtc gaggttcttt gggtcaaagg
     4741 gtcgggcggc gatctcggga cgatgaagct ggacggcctc tccacgcttt atatggagaa
     4801 gtttctcggg ctggagaagt tttacggttc gtcgaaagcg gccggcaagg atcccgacgg
     4861 cacgctggtt cctttgtacg cgcacagcgc tttcaacctg aatccgcgcg cggcca
//
LOCUS       DASQJS010000017         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2839150, whole genome shotgun sequence.
ACCESSION   DASQJS010000017 DASQJS010000000
VERSION     DASQJS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2839150"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VEW08_00715"
     CDS             complement(<1..528)
                     /locus_tag="VEW08_00715"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HYJ39273.1"
                     /translation="MRKWVMGFIVVLASVNLAGCGYNKLQNQDEGVKAAWSEVVNQYQ
                     RRADLIPNLVAAVKGFAAQEERVLVGVTEARARASSIQITPEVLNNPETFAKYQEAQA
                     QITTALKTLLATVEAYPDLKSDANFRDLQAQLEGTENRITVARNRYIESVKEFNVTVR
                     EFPTNITANMFDYDVK"
     gene            complement(559..2631)
                     /locus_tag="VEW08_00720"
     CDS             complement(559..2631)
                     /locus_tag="VEW08_00720"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HYJ39274.1"
                     /translation="MLNRIVVVALAAVFTAVALAACASVPKPATPPAPVPAPAPPLKS
                     GLDLAGFDRSVRPQDDLYKFAGGTWLAKTPIPADRSNYGSFIILDDQAQEEVRQLIVA
                     ASTQANRPTGSDAQKVGDFYLAYMDNNRVESLGLTPLKDELARIDAIATPRDVARYIG
                     YSQRIGLAQPFAWFSSPDNKNSTVYLGSLYQNGLTMPDRDYYLSPDEKYAVFRARFAE
                     YVEQMLARAGERNAKSAAARIAALETRLANYQWTKVQNRDPVKTYNPLTLPEYQKLAP
                     GFDWLAFFEGMGAPVQRLDVNQPTFINGIAQLVKTVPVSDWRVYFKFQLLDDYAPALP
                     AQFADLEFGFHQKVLNGVPEQRPRWRRAVDSMDNNMGELVGRLFVESHFGAEAKAKML
                     ELVGNLLKAFDSSIDGLEWMGPETRIEAKKKLAKITVKIGYPDKWRDYSALTVRSDDL
                     VGNLLRASEFQHNRYVARTGGPVDKGEWLMTPQTVNAYYSSATNEITFPAAILRWPFY
                     DMAADPAVNYGGIGSVIGHEISHGFDDSGRQFDGDGNLRDWWTADDGAKFKQRAAALV
                     SQFAGYSVLDGKHLNGELTLGENIGDLSGMAVAFKAYKLSLDGKTAPEIDGFTGDQRF
                     FLGWAQVWRRNYRDAELLKRLVTDPHSPSEFRANGPSSNIDAFYDAFGVKPGDLMYRP
                     PAERVKIW"
     gene            complement(2774..2968)
                     /locus_tag="VEW08_00725"
     CDS             complement(2774..2968)
                     /locus_tag="VEW08_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39275.1"
                     /translation="MAARYYTHFVTSAEPAHSSEWSGVIELTHPLGERRDSKELRRLL
                     AANFEIDAEDIKILQVARLH"
     gene            3121..3576
                     /gene="greB"
                     /locus_tag="VEW08_00730"
     CDS             3121..3576
                     /gene="greB"
                     /locus_tag="VEW08_00730"
                     /inference="COORDINATES: protein motif:HMM:NF002506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HYJ39276.1"
                     /translation="MTSAGAARLKQELDELWRVERPRVTQAVSEAAAQGDRSENAEYT
                     YGKKRLREIDRRVRFLRKRLDGMTVVDKSPSDPRRVFFGAWVTLEDDDGKQTKYRIVG
                     PDEFDQAPGYISMDAPLARALMRKALDDEVKVTLPGGERAYVIVEISYQ"
BASE COUNT          710 a         1138 c         1155 g          699 t
ORIGIN      
        1 cttcacgtca tagtcgaaca tgttcgccgt gatgttggtg gggaactcgc gcaccgtcac
       61 gttgaattcc ttcaccgact cgatgtagcg gttgcgcgcc acggtgatgc ggttctccgt
      121 gccttcgagc tgagcctgca ggtcgcggaa gtttgcgtcc gacttgaggt ccggatacgc
      181 ctcgaccgtc gccagcagcg tcttgagcgc cgtggtgatc tgggcctgcg cctcctggta
      241 cttcgcgaag gtctcgggat tgttcagcac ctcgggcgtg atctggatgg agctcgctct
      301 cgcgcgcgcc tccgttacac ccacgagaac gcgctcttcc tgcgcggcaa aacctttcac
      361 cgcggccacg agatttggaa tgaggtccgc tcgccgctgg tactgattga ccacttccga
      421 ccacgccgcc ttcacccctt cgtcctggtt ctgcagcttg ttgtaaccgc agccggcgag
      481 attcacgctg gcgagcacga ctatgaaacc catgacccac ttacgcatac gaatctccgt
      541 ccacgtccga aaaccaactc accagatctt tacacgctct gcgggcggtc gatacatgag
      601 atcgcccggc ttcacgccga acgcatcata aaacgcgtcg atgttgctgc tgggcccatt
      661 cgcacgaaac tcgctcgggc tgtgcggatc ggtgaccagg cgcttgagca gttcggcgtc
      721 gcgataattg cggcgccaga cctgcgccca gcccaggaag aagcgctggt cgccggtgaa
      781 gccgtcgatc tccggcgcgg tcttgccatc gagcgacagc ttgtaggcct tgaacgccac
      841 cgccatgccg gacagatccc ctatgttctc gccgagcgtg agctcgccgt tcagatgctt
      901 gccgtcgagc acggaatatc cggcgaactg actcaccagc gcggccgcgc gctgcttgaa
      961 cttcgcgccg tcgtcggccg tccaccaatc gcgaagattt ccatcgccgt cgaattgccg
     1021 gccggaatcg tcgaaaccgt ggctgatctc gtgaccgatg accgagccga taccgccgta
     1081 gttcaccgcc ggatctgccg ccatgtcgta gaacggccag cgcaggatcg cggccggaaa
     1141 ggtgatctcg ttggtcgcgg agctgtagta agcattcacg gtctgcggcg tcatcagcca
     1201 ctcgcccttg tcgaccggac cgccggtgcg cgccacgtac cggttgtgct ggaactccga
     1261 ggcacgcaac aggttgccga ccaggtcgtc gctgcgcacc gtcaacgcgc tgtagtcacg
     1321 ccacttgtcg gggtagccga tcttcacggt gatcttcgcc agcttcttct tcgcttcgat
     1381 gcgtgtctcc gggcccatcc actccagccc gtcgatcgag ctgtcgaacg ccttgagcag
     1441 gttgccgacg agctcgagca tcttcgcctt cgcttcggcg ccgaagtgcg attcgacgaa
     1501 cagccggccg acgagctcac ccatgttgtt gtccatggag tccaccgcac ggcgccagcg
     1561 cggccgctgt tcgggcacgc cgttcaggac tttctggtgg aagccgaatt cgagatcggc
     1621 gaattgcgcc ggcagcgccg gcgcgtagtc atcgaggagc tggaacttga agtagacgcg
     1681 ccagtcggac accggcacgg tcttcacgag ctgggcgatg ccgttgatga atgtgggctg
     1741 gttgacgtcg aggcgctgca ccggcgcgcc catgccttcg aagaaggcca gccagtcgaa
     1801 gcccggcgcc agcttctggt attcgggcaa cgtcagcggg ttgtaggttt tcaccggatc
     1861 gcggttctga accttggtcc actgatagtt ggcgagacgg gtttcgagcg ccgcgatgcg
     1921 cgcggcggcc gacttcgcat tgcgctcgcc ggcgcgcgcc agcatctgct cgacgtactc
     1981 ggcgaacctg gcgcggaaca ccgcatattt ttcatcggga gaaaggtagt agtcgcggtc
     2041 gggcatcgtg aggccgttct gatacaaact gcccaggtac accgttgagt tcttgttgtc
     2101 gggtgacgag aaccacgcga acggctgtgc gagcccgatg cgctgtgaat acccgatgta
     2161 gcgcgcgaca tcgcgcggcg tggcaatggc gtcgatgcgc gccagttcgt ccttgagcgg
     2221 agtgagcccc agtgattcca ctcgattgtt gtccatgtag gcgagataga agtcgccgac
     2281 cttctgcgca tcggagccgg tggggcggtt tgcctgcgtc gacgcggcca cgatgagctg
     2341 cctcacctct tcctgcgcct ggtcgtcgag gatgatgaac gagccgtagt ttgaacgatc
     2401 ggccggaatc ggcgtcttcg cgagccacgt gccgccggcg aacttgtaga gatcatcctg
     2461 cggacggacg ctgcgatcga agccggccag atcgaggccg gacttgagcg gtggcgcggg
     2521 cgcaggaacc ggcgccgggg gtgtggcggg cttgggcacg gaggcgcacg ccgcgagtgc
     2581 gacggccgtg aacacggctg ccaaggccac gacgacgatg cgattcaaca tggaggtccc
     2641 cggacaaata gctgcgatgg gaccgagtat aaaaaaagcc ccaagcttta ggggctcggg
     2701 gcttgagaag gcggactttc gatgaaggga aagaaggtcc gctaggggat ctctacaaaa
     2761 tcaaaaaaac agttcagtga agtcgtgcca cttgcaaaat cttgatgtcc tcggcgtcga
     2821 tctcgaagtt cgctgccagc aagcggcgca gttccttcga atcccggcgt tcacccagcg
     2881 ggtgtgtcag ctcgattacg ccgctccact cactcgagtg cgctggctca gcgctcgtga
     2941 caaagtgcgt gtaataacgg gctgccatgg gtagaaagcg tagattgacg gctccaaaac
     3001 ggctgtgagc cgctgcacag aacccttcgg cagactgccg ccgtgactga gacccccatc
     3061 acatatgttt tataagtcgt gagtcgctac cgagctacag agaaacccgg atcgcaattc
     3121 atcaccagcg caggcgcagc acgcctcaaa caagagctgg acgagctgtg gagggtcgag
     3181 cgcccgaggg tcacccaggc ggtctccgaa gccgccgctc agggcgatcg ctccgagaac
     3241 gccgaataca cctacggcaa gaaacgcctg cgtgaaatcg accggcgcgt gcgcttcctg
     3301 cgcaagcgcc tggacggcat gaccgtggtc gacaaatcgc cgtcggaccc gcgacgcgtg
     3361 ttcttcggcg catgggtcac gctcgaagac gacgacggca aacaaacaaa gtaccggatc
     3421 gtgggacccg acgaattcga ccaggcgccg ggctacatca gcatggacgc gccgctggca
     3481 cgcgcgctca tgcgcaaagc actggacgac gaggtgaaag tgacgttgcc gggcggcgag
     3541 cgcgcctacg tgatcgtgga aatttcctat caataagtga agtccggggg ttgcccgccg
     3601 gctgcaatgg ttgtaatttc tgtaaagaca gaaattacag gcggcacgat atgcacctca
     3661 ccgaaaccat gcagcgctac atcgtccact ggggcgaaat gg
//
LOCUS       DASQJS010000018        28913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3466007, whole genome shotgun sequence.
ACCESSION   DASQJS010000018 DASQJS010000000
VERSION     DASQJS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 28913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28913
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3466007"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VEW08_00735"
     CDS             complement(<1..504)
                     /locus_tag="VEW08_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39277.1"
                     /translation="MIVTDQFVFLHLHKSGGSFVNEFLKRFFPSAREIGYHLPWSLVP
                     ASHAHLPALGLVRNPWGYYVSWYTFQKQRPQQNALFRLLSDDGRLDFEPTVRNMLDLG
                     TTGAGLDALVAALPQGYGGRGLNLPGPALATIAGSGLGFYAFLYRHMYNGAGRAYFVR
                     MEHLRDEL"
     gene            complement(501..1139)
                     /locus_tag="VEW08_00740"
     CDS             complement(501..1139)
                     /locus_tag="VEW08_00740"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HYJ39278.1"
                     /translation="MLDLPGHPPLDKLGLIGTCSRLPVQVNAARLQAEVAALPEGSWA
                     GTGGRVGVHRAAQAIFLRGFAPAEGEKPIEDREHLFDMPYVREIIAELIPAPAQRCLL
                     ARLPPAARITGHIDLAPYFAKTIRIHVPVVTNADAWMYCGGLAFRMQPGEVWALNNSS
                     THGVWNAHTTEARTHLICDFLPSPELLGLLMRAERELGAPNAELETMMRGAH"
     gene            complement(1177..2457)
                     /locus_tag="VEW08_00745"
     CDS             complement(1177..2457)
                     /locus_tag="VEW08_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYJ39279.1"
                     /translation="MKRIGTLVLAATLCATAFTTGTAFGGAAEGSWFIAPRVNALWLD
                     DGRLADDDVGVTLGFGYTMTEKWDVEVSLYGSEHKQVGDANLELQGYGLSLNRVFYRE
                     GRVNPFLSLGIAQTKSIHKPGVADEVMTGLYGGGLLITLGADRDDGSVMQLRADLGAR
                     RALTTDTTEEYPVDYVAALGFQYSWGGSRRVLDSDGDGVNDNLDKCPGTPAGTAVDSS
                     GCPLPQDDDGDGVTNDIDKCPGTTAGAKVDANGCELDSDGDGVGDSRDQCPNTPAGAK
                     VDERGCELDGDGDGIVDSVDKCPDTPKGERVDNTGCPFNKELLLQGVKFETNSAALLP
                     ESIPVLNNAIATLKRYPEVNVEVAGHTDSRGSDAFNLDLSARRAEAVLKYLQDGGVSN
                     TMTSRGYGERQPIASNNTDDGRQQNRRVTLRILN"
     gene            complement(2491..3003)
                     /locus_tag="VEW08_00750"
     CDS             complement(2491..3003)
                     /locus_tag="VEW08_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HYJ39280.1"
                     /translation="MRLTLNRPDRLRRWCLALAAAMAAAGSTPALAQNGPLEDLAAFP
                     RGKLEISDGKKVKLTFDVWLADSPSRQAQGLMFVRALPDLRGMLFVHESPKPISMWMK
                     NTYIPLDMLFIDSRGRIQQIVEQATPHSLDIIRSNEPAVAVLEIAGGEARRLGLHPGQ
                     HVAHPALAKR"
     gene            complement(2978..3604)
                     /locus_tag="VEW08_00755"
     CDS             complement(2978..3604)
                     /locus_tag="VEW08_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39281.1"
                     /translation="MPHRASAAALIAALGAAFAHTACAAPPASPVTDPAEKTGCFAAG
                     NGYLRARIRGAVDLDLDWKDAQMQCEGGPRPPGKDNQSNGIRVSIAGPLRGDGRRIRL
                     VFGIGGVVEGRGGETLRTNVTILFEGEHRIFATLGDDKCTVDSLTQQRVESLGADRAI
                     YRVEARGFCLGAATSLSKGERVLLTSFDFAGRVEFEDDARHAPDPQSP"
     gene            complement(3609..4553)
                     /locus_tag="VEW08_00760"
     CDS             complement(3609..4553)
                     /locus_tag="VEW08_00760"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HYJ39282.1"
                     /translation="MTDNAPRSVEDYLRQLRAALANQDIALIQDALYDAEEYLRAEIA
                     SHPDKSEADVLELIASTYGAPEEVAAAYRDTETKLNAALRTPRAPPRASMLGRFFGIF
                     LDPRAYAGLLFMMFSLATGILYFTVAVTGLSLSAGLAFLIIGVPFFLAFIGATRALAL
                     VECRLVEAVSGVRMPRRPLHPGAPSGFWDRVKAMVTDSRTWTTLAYDLLMLPLGVAYF
                     MMAVVGLSFGLALIAAPVIEGLRALGAFSDQSVFAHGVHPVHNGVVVDMNPAWLGTPG
                     GLVLLGIVGILLLTATLHLARGVTKAHAVLAKSMLVLP"
     gene            complement(4546..4911)
                     /locus_tag="VEW08_00765"
     CDS             complement(4546..4911)
                     /locus_tag="VEW08_00765"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYJ39283.1"
                     /translation="MDDSHAKKFQKDLNGGLVSLVLLAVLERSEEDLYGYDIVKRLQR
                     ANEGELLFKEGTVYPVLRALSANGLLSSRIVPSYSGPPRRYYRITDEGRTVLGSWRGI
                     WRDAREFVDRFVESGASND"
     gene            5036..6301
                     /gene="glgC"
                     /locus_tag="VEW08_00770"
     CDS             5036..6301
                     /gene="glgC"
                     /locus_tag="VEW08_00770"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HYJ39284.1"
                     /translation="MAKRPARATSGRYVSRLTSGTLAVIMAGGRGERLKGLTEHRAKP
                     ATPFGGKFRIIDFVLSNCVNSGIRQITVLTQYKAQSLIQHIHRGWGYMRGEFGEFVEV
                     IPAQQKTGTHWYRGTSDAVYQNIDVMHSHRPKHVLVLAGDHIYKMDYGPMIAYHVEKG
                     ADITVGVVEVPIVEAKEFGVLTVTEWNRVTRFVEKSPNPDPMPGRTDVALASMGIYVF
                     NPELLEKLLIEDAADEKSKHDFGKDIIPKSIEKLQVFAYPFRDVKTRAQSYWRDVGTI
                     DAFYEANLELVWVAPELNIYDEDWPIWTYQAQTPPAKFVLDEDGRRGVAINSLVSGGV
                     IISGATVRESLLFSGVRVEERSVVERSVILPDVRIGQNCQIRNAIVDAGCEIASDMRI
                     GFDRAEDARRFLVTEKGVVLVTPDMLSHA"
     gene            6294..6773
                     /locus_tag="VEW08_00775"
     CDS             6294..6773
                     /locus_tag="VEW08_00775"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HYJ39285.1"
                     /translation="MREQRGARRLALVALASLTLLSLVGGGCAMVPQLEAPKLSVVSL
                     KMQGGDIFSQRVLVRMRVINPNSRELPIKGLTYSIEVNDAELGNGATAAPFTVPAMGE
                     AEFDMQITANLAGALAKLLSRRSSSEALEYRLRGNVSLSSGFLRRIPFDERGSVKLK"
     gene            complement(6711..8231)
                     /locus_tag="VEW08_00780"
     CDS             complement(6711..8231)
                     /locus_tag="VEW08_00780"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HYJ39286.1"
                     /translation="MPAAVKLSICAIASEVVPLAKSGGLADVASALSRQLTVAGHDVR
                     LFMPFYSQVRRDKLDAKRVETLQGLRILAGNHEYLVDIWRATLPGSASPVYLVESDAL
                     YARPKLYTGDADEHRRFLVLTRAALECCRRMNFRPHIVHSHDWHTAFAPLWLRSNYKS
                     DPVFAGTKSVMTIHNIGYQGAFSALDIADLGLGADTYLLHQDDLRAGHINALKHGILH
                     ADAVTTVSPTYAREITTPRYGMGMEQALASRGSAVHGILNGVDYDEWDPAHDKHLPIH
                     FGPGNIAPKAELKRNFLLRQGMVPTRGRVPLFGIVSRLASQKGFDLLTDTLPALLQNN
                     DCRLAVVGTGDRLYEDFFAGLAAKYRGRAWFFGGYDEALAHWIEGASDAFLMPSQYEP
                     CGLNQMYSLRYGTVPIVRRTGGLADSVTHFDPATGEGTGIVFNDFDATAMTWALDTAM
                     SWYAQPSLWERIVQNAMRCDFSWETQANEYLKLFAELLQLDAAALIKRDPAQKAAR"
     gene            8281..9453
                     /locus_tag="VEW08_00785"
     CDS             8281..9453
                     /locus_tag="VEW08_00785"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYJ39287.1"
                     /translation="MRRVLTFLTAIVLLLVSLGVGVFTADLPFWRRALQLPLPADGAY
                     LPVARIGTPESPPPHPPHREPANPPAPPIDALVVEEAANRAGNSGSRALLVLYRGSLA
                     VERYFLTDDAHTLLPAALVARPIAAMAIGIARADGKIASLDDPVARYLPEWDDEARGR
                     ITLRQLLEETSGLETGGDTRGLLHRSPWREPASLPVFATSRGVRMLLGNDFQSSALGF
                     RLEHEPGGFYNLSPANTQLAAVIIERVTGEPYENFVDERLWRPVGAGLAEMQLDRRAG
                     MPAAHCCWRATARDMMRILSLLGTDGVHDGRAVLPAGWASEMARASRVSAGTGMQVTR
                     LSIDGAEALEVRDEAGSAFWVIPQRQLAILNITNPGGSAVTDLPAMLLKGIEAPAP"
     gene            9507..10373
                     /locus_tag="VEW08_00790"
     CDS             9507..10373
                     /locus_tag="VEW08_00790"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HYJ39288.1"
                     /translation="MDMKVTSISDAFVDSREFARMTRAIEFIEREYQRQPKLAEIARF
                     VGLSEFHFNRLFRRWTGLTPKQYLAEVTSRAAGTALRNEPSVLDAAHSVGLSGGGRLH
                     DLTVTLEAMTPGELRAGGSGVTIRHGIANTPFGTAFFAETPRGLCRLAFIDGPGDGKE
                     LKLLRGQWPKAKFVRDDERATTLVETIWGHKTVTLPLAVCGTNFQVQVWRALLELDAT
                     ESVSYSALARRLGLPDGARAVGNAVGANPIAWVIPCHRVLRAGGQLGGYRWGAARKQM
                     IRRWEERRTGVS"
     gene            10455..11612
                     /locus_tag="VEW08_00795"
     CDS             10455..11612
                     /locus_tag="VEW08_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ39289.1"
                     /translation="MSEKRSLTTPISIAVAVVCAAWAIYATQHKPAAGSGGPPAAAGR
                     NNAPAFVTLAPVRTERISQKLEALGNARANESVDISTKLSNIVTAVTFRDGERVKRGQ
                     VLVQLDNAQARADVAAAQAAVTESESLYNRSRELLNTQALSKSSFDQLEATLKANRAR
                     LGAASARLEDTVIRAPFSGRVGLRRVSVGTLISPGDVITTLDDTSVIKLDFAVPENFL
                     ASLREGLTIRATAPAFPGRTFAGSVSSIDSRVDIATRSVTVRALLTNEDGALKPGMFL
                     NVSLANDEREALVIPEEALTPEAERQFVFVVTDGKVSRREVRIGGRRPGSVEILAGVV
                     AGERVIVEGTQKVRDGAMVQAAERASEADPAGPGAVSESHEANASPARIAQ"
     gene            11609..14797
                     /locus_tag="VEW08_00800"
     CDS             11609..14797
                     /locus_tag="VEW08_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYJ39290.1"
                     /translation="MMISDLSVRRPVFATVMSLLLFILGVGAVTRLSLREFPDVDRPV
                     VNIETRYRGASSDVVESRITQVIENEISGIEGVERLNSGSRDERSQINVEFSLDRDLD
                     SAANDVRERLSRVAGRLPLEADPSQITKVDSGTDPIVWINVTSTQRSILELTDYMERF
                     LVDQFSTLEGVASVRVNGGRRYAMRVWLSRENLASRQLTVNDVENALLRENVELPAGR
                     LESTEREFTLRTDTNLRTEADFRNLVVGRGADGYLVRLSEVAEVRLASEDDRSISRSN
                     GVIGMSMAVLPQSKANVLATSAAVKKQLASIQSTLPKDIVAEINIDNGMFIAASLKNV
                     VVALSETLVLVLVVIFVFLGTLRATLIPAVTIPVSIVAAAIVMLALDYSINTLTLLGA
                     VLAIGMVVDDAIVVLENIVRRIEGGEPAVLASINGSREIGFAVIATTLVLMAVFVPVS
                     FMTGDTGRLFGEFGLTVAAAVGFSAFVALTMTPMLTSKLFAGGMPKSRMTGGIDRMFR
                     NLDEKYARGLRSALSGKRPLWIVSGSLALFGVVATLMIQGFPFSALRLPSELAPEEDR
                     AFVRIFVGAPEGASLSYLDRQLRQVEEIAQDEVERGNAKRVIVRTGGFGRAAETNTGF
                     VALPLNLWDERDDSAGLIADRLRQRTQNITGARVNVNQAGSPLTRNSTRPLAIALGGG
                     DYAEIVKWRDKIMNRVEAENPRILDLESDYLERKPRVIIQIDRNKAADLGVSLQSVGR
                     TLETMLGSRIVTTFERSGEEYDVILQARDEQRATVGDLDNLYVRSDKDGDLLPLSALV
                     RIEERAGPNELRRTDRMRAIELAGSLAPGYALGDAVKYMEQVIREEAPEAQIIYNGQT
                     YQLKQSGSTLWITFAFALLVVYLVLAAQFESFIHPVVILVTVPLAITGALIGLWLFNS
                     TINIFSQIGCIMLIGIACKNGILIVELANQLRDRGVEFVDAVIQAASVRLRPVLMTSL
                     CTAFGAVPLMLAHGAGAESRESIGATVFFGTVVSLGLTLYVVPSLYLLIARNTRSPEH
                     MTRLIDALLGGRPARGTPATAESAAAPSAERP"
     gene            14986..16509
                     /locus_tag="VEW08_00805"
     CDS             14986..16509
                     /locus_tag="VEW08_00805"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HYJ39291.1"
                     /translation="MAQSPYKQLEQEWKRLHAFRGAMAILRWDAAVMMPRGSADVRGE
                     QLAALETEYHALVTAPRITRLLDRAQANTGGLEDWQISNLREMRRQRDFAIATPVALV
                     SRLAKAASKAEAKWLHARETGKFEDFAPHLEEVVHLVRDKAQLLGQARNLAPYDALVD
                     EFSPGLTSGEIDAMFKSLARRLPGLIREVLVRQEQRPVLPIVGKFSAGKQRALALEIM
                     KAVGFPFDRGRLDESEHPFTEGVPGDVRVTTRFDTTEPFSGMLGALHETGHAMYDVGL
                     PAEYSDQPVGRDRGMALEESQSLLMEMIVCRSRGFVRYLKPLLEKHFGVSGPEWSEEN
                     LYAHLTRVKRGPIRVDADELTYTLHIQLRYELENQLLSGELAVRDLRDAWNSSMEQRL
                     GVKPQNDLEGVLQDVHWAVGSFGYFPSYGLGAVMAAQFYEALHEAVPDVETQIARGDF
                     GGLMGWLRENVHRHGARVTTQELLKLSTGKPLSAVSALRYLEAKYLEPESVVGSAAA"
     gene            16529..17299
                     /locus_tag="VEW08_00810"
     CDS             16529..17299
                     /locus_tag="VEW08_00810"
                     /inference="COORDINATES: protein motif:HMM:NF017672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HYJ39292.1"
                     /translation="MSQWRGWLVGAISTVFFLAALLVPPMPQPLSYHVFADCRSFFGV
                     VNFFNVVSNFPFLIGGVLGLQLIWSGRVTFIDPREQLPYIVFFLGALLTCFGSAYYHG
                     APDNARLVWDRLPMTLGFAGLVAATIAERVDSKLGRWSLWPLLLLGVFTVVYWYGSEL
                     RGSGNIIPYGAYQAWSVLIIVLLLLAYPARLYSHGGLLGWAAAWYGLAKVFETFDLQI
                     YRLLGSTLSGHTIKHVLASFAVFAIVRQLRVRHRIPRP"
     gene            17296..18153
                     /gene="ttcA"
                     /locus_tag="VEW08_00815"
     CDS             17296..18153
                     /gene="ttcA"
                     /locus_tag="VEW08_00815"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HYJ39293.1"
                     /translation="MSAQPENILDSPEGLETSPEWPSQTAKKLGAHLRGLTGKAIEDY
                     RMIADGDRVMVCLSGGKDSYTLLDVLLSLKRSAPIDFDLIAVNLDQKQPDFPAEVLPN
                     YLRGLGVPFHVIEQDTYSVVKRVIPEGKTMCGLCSRMRRGALYRYAAENGITKIALGH
                     HRDDIVETLFLNMFHGGRLKAMAPKLKSDDGRHIVIRPLAYVAESLIERYARARQFPI
                     IPCKLCGSQDNAQRKEVKRMLAEWERQYPGRTESIFSALRNVEAAHLADPRAFDFPGL
                     DAERTQIPE"
     gene            18125..19609
                     /locus_tag="VEW08_00820"
     CDS             18125..19609
                     /locus_tag="VEW08_00820"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HYJ39294.1"
                     /translation="MPNAPKSLNKPIGESYWVIEDRLLAGRYPGGKTPKEAERRVASL
                     IDAGFDAFIDLTEPGELPPYDLYLPGSALYVRKPIPDHGVPGAPEYMAGILSELDAAL
                     ASGRRVYVHCRAGIGRTGTVIACHLIEQGLSPDAALIRLNELWQGNDLSATWSDVPET
                     DEQHEYVRAWTPAADPTKQPDVMDAARTLRERFQGALVGLAVGDALAAHTQFRKPGSF
                     AAVGDLLGGGPFDQPRGAWTDDTAMALLLAESLLAREGFDARDQVERYVRWQREGYGS
                     ATGQCVGISANVARSLATAQYKRQPFAGSHDPGQLDKDPLSRIAPVAMYFFADASTAV
                     AKAAESARVTAQAPMVLDCVRLLAAMVHLALSGRDKATLLHPPRDLWATASTRPEVLA
                     LYDGAYAHRMPPDITGGGGIFQALEAALWAFHRTESFREGALLAANLGRDSDVVAAAY
                     GQIAGAYHGVSAIPGIWRNSLMKQEVVIDSADRLLTHALVTLGS"
     gene            19646..20689
                     /locus_tag="VEW08_00825"
     CDS             19646..20689
                     /locus_tag="VEW08_00825"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HYJ39295.1"
                     /translation="MPSTRTSRGSLAKRSWTRLSDEQLLGLRFCDLKLKIERSPVAKR
                     VRRLYRELDKRQISFRPHVWLSEEWFSPDGVPGIAVPFYLAHPRLERLERRMMRNVEG
                     GSAESAMRILRHEAGHAIDTAYRLRRLKRWREIFGPASLPYPDTYRARPGSRRYVQHL
                     GEWYAQAHPCEDFAETFAVWLKPNSSWRRTYAQWPAFHKLEFVDELLEKVRGTRAPVR
                     NREVVEPLRENTHTLADHYRRKLRRYSMYRRTVTDHLLERVFTAERLRRASRASTFLR
                     AHSLHLVNATMRELHAERYSVEQILRIVVERADKLGVWVRGSRRDALRHARWMLVYLT
                     RLYAQGESPRLSL"
     gene            20686..21720
                     /locus_tag="VEW08_00830"
     CDS             20686..21720
                     /locus_tag="VEW08_00830"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39296.1"
                     /translation="MKRLRTLVVVHSTLVPPDSLEGHSEKEIEEWRTEFDVVSTLKKS
                     GHEVRCLGISDSVEELRSVITEWKPEMVFNLLEEFDGIVTYDQHVVAFLELLRQPYTG
                     CNPRGLLLSRDKPLCKQLLSYHRISSPQFAVFRRGIKLRLPRKIKYPLFVKSTTEDAS
                     LGISQASIVTDKAQLKQRIQFVHDQVQTDALVEEYIEGRELYVGVMGNDRLTRLPVWE
                     MKFGSLAETSAGIATRRVKWDKKYQKKHGIDTHEAKDLPPAVVARLDKLSRRVFRSLG
                     LSGCARIDYRMNAEGQIYVIEANPNPNLAADEDFAQSALAAGVSYPELLEKLLTLGAG
                     YQAEWRAYYG"
     gene            21754..22239
                     /locus_tag="VEW08_00835"
     CDS             21754..22239
                     /locus_tag="VEW08_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39297.1"
                     /translation="MKNWSHRAVVGTFLLALVPALSGCMVSAAEMQDNITKFRASTSG
                     QYKSEAGAKLYIAPVRARMVTDEALYLERHDPNGTIVGRLLNVEVAEDGKTIVLRALV
                     FTQEGQWRNLRENPELFTALLPKDVRPAGKCDIKPSKDRDSLTYSCGGSPVENFTRVT
                     E"
     gene            complement(22259..24304)
                     /locus_tag="VEW08_00840"
     CDS             complement(22259..24304)
                     /locus_tag="VEW08_00840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYJ39298.1"
                     /translation="MKSWMCIAAVLAGSAQAGAADAPSRALTPTDLNMLTRVSDPQVS
                     PDGRYVVYVQRTTDLEANRGRTDLWLLDLTNAKPKPRQLTQHSSNDAHPRWQVDGSSV
                     YFLSSRTGSQQIWRLPLTGGEAVQITDYPLEVGTFRFSPDGRRIALSMEVFPDCVDAK
                     CTRERLDAAGKKKETGRVFDGLFVRHWDTWSTGLRSNLFVAPVNADGRAGPPVNVSRA
                     LNADVPSRPDGGDEEFRFSPDGSRVVFSARVAGREEAWSTNFDLYEVPSDGGAAPRNL
                     TPDNPAWDTQPVYLRNGDLAWLAMSRPGFEADRFRIKVRSGASVREVAPGWDRSVQHL
                     ELASDGRTLLATASDFGQTPLFSVDVATGRATPISGKGNVGDFAPAKRGAVVLWHDLA
                     SPPDLYLQTGNSERQRLTTANAEVLANRTLGSFEQFSFPGWNNETVYGYLVNPPGFAS
                     DKKYPIAFIVHGGPQVSFQNHWNWRWNAQTFAARGYATVFIDFHGSPGYGQAFTDSIS
                     QHWGDRPFEDLKKGLAAALQKFPWLDGTRACSLGASYGGYMQNWIAGNWPDGFRCIVN
                     HDGIFDTRSMYYTTEELWFTEWENGGPYYQTPEIHERFNPANHVKQWRTPMLVIHGEQ
                     DFRVPDTQGIATFTALQRLGIESRLLYFPDENHWVLKPNNSIQWYGTVLDWLDSHTK"
     gene            complement(24331..24984)
                     /locus_tag="VEW08_00845"
     CDS             complement(24331..24984)
                     /locus_tag="VEW08_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYJ39299.1"
                     /translation="MDEILLTMGNKNYSSWSLRPWLLMKHVGLDFAERVMPLDTPEFA
                     RDVAAASPTRRVPVLRHGALLLWDSLAICEYACELAGRGWPRAREARAIARAACAEMH
                     SGFSILRSQWPMNARATGRRTAPNPERSAEIARIENLWADCRARFGAGGPWLFGDFSV
                     ADAMYAPVVLRLRTYGATVRDDTAAYMATVLADEHMLEWLAAAAAESWIIEASEIGC"
     gene            25012..25710
                     /locus_tag="VEW08_00850"
     CDS             25012..25710
                     /locus_tag="VEW08_00850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYJ39300.1"
                     /translation="MSIGAIRAICFDLDNTLWEIEPVLNRAERILADWLERRYPRIRE
                     RFTAAEVLEVRAALLVEQPHQAHDLSFLRRETIARCATEVGYKRDMAHEAFSVWHAAR
                     NQVEPYAEVIPALEKLKARFRLASLSNGNADLKVIGLAHHFEVTLSAGALGYAKPDPR
                     AYTALAEALTLKPAEILFVGDEPHADVAGPRTAGMQTVWVNRGGVVWPDALPAADASV
                     TDLAGLTALLTRNL"
     gene            25898..26530
                     /locus_tag="VEW08_00855"
     CDS             25898..26530
                     /locus_tag="VEW08_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39301.1"
                     /translation="MAIQPVASLKEFFRDALHDALSHQHVAVEGETEHYVVNVLTLFS
                     DADALYERQSYNEARHADSRRQLKPLAMMLGEALEAPTSEARFRGLQRLGDVSLFIAG
                     FFSAGFARKLVDVDYHIAMGGQAYGALAESCPPARARTLRQVFAELAAKFQPMVDALN
                     EISETAYQHSDQDRLRLYELWVKTGSERSRQLLRKLGLEPAPAARSLLSH"
     gene            26532..27191
                     /locus_tag="VEW08_00860"
     CDS             26532..27191
                     /locus_tag="VEW08_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39302.1"
                     /translation="MNDATLSSLQGALTEIYDLPATPDVRDFLLTDRARLSAVGGPRA
                     SDEQLIVAEEGDTLSLALYIDAAVLERLERCDPFAGLTQHNLADYLTVAEGVSHFVYV
                     AWNTGYDKPVTLLELELQAEVDKYVLCAWLLRKQGAGRFPRELHRILFERAHVDPVAA
                     AGRIGLYHTASDYAARFCRRVAALLERKRNGVARDLMAELRRFYRWGNVRKMHHIERY
                     A"
     gene            complement(27220..27585)
                     /locus_tag="VEW08_00865"
     CDS             complement(27220..27585)
                     /locus_tag="VEW08_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39303.1"
                     /translation="MSDNNSNSKPPQAANRRRVSHIVHDDRGNARVEWIDAGYAGVPL
                     ERAPLSIESTPPRGDNAKLTVERKRAGGFDPYARVGAAHIPESRKPPVKRDLRKLGEW
                     IKLKRELDERKSQDDHDVE"
     gene            complement(27670..28329)
                     /locus_tag="VEW08_00870"
     CDS             complement(27670..28329)
                     /locus_tag="VEW08_00870"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYJ39304.1"
                     /translation="MSAGRRPVQASPCLLCGRIERDKQLFRHRLTTVGPAVRDGEYTR
                     AMLFDRKEIFEGRVIKVSVDTVDLPNGVRLPLEIVRHPGGAAAVAIDAGNRVCLLRQF
                     RHAAGGYIYELPAGKLEPNEPPEVTVQRELAEEAAISARQWESLGAYFSSPGVFTEVI
                     HLYLATDLTPVESAPEDGEVFEVEWWPLEDAVAKARSGELNDAKTIIGILRAAARRKI
                     A"
     gene            28478..>28913
                     /locus_tag="VEW08_00875"
     CDS             28478..>28913
                     /locus_tag="VEW08_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HYJ39305.1"
                     /translation="MSTAEQLGSLNAPQRKAVTYGEPLPGGKGFRAGPLLIVAGAGTG
                     KTNTLAHRVAHLAMSGVDPARILMLTFTRRASIEMKRRANEIMREAFDEKLGGGKAQA
                     ISQRLTWAGTFHSIGNRLLRHYAKHVGLDPAFSVLDRSDSADL"
BASE COUNT         5120 a         9244 c         9302 g         5247 t
ORIGIN      
        1 cagctcgtcg cgtaaatgtt ccattctgac gaagtacgcg cgcccggcgc cgttgtacat
       61 atggcgatac agaaacgcat agaacccgag accgctgccg gcgatcgtcg cgagcgccgg
      121 acctggcaaa ttgaggccgc gacccccgta tccctgcggt aaggccgcca ccagcgcatc
      181 gaggccggcg cccgtggtgc ccagatcgag catgttgcgc acggtcggct cgaaatcgag
      241 gcgcccgtcg tcgctgagca ggcggaaaag ggcgttttgc tgtgggcgct gcttctggaa
      301 cgtgtaccag gagacgtagt aaccccaggg attacggacc aggccgaggg ccggcagatg
      361 cgcgtgtgaa gccggcacca ggctccaggg aaggtggtag ccgatctcgc gcgcggacgg
      421 aaaaaaccgc ttgaggaatt cattgacgaa actgccgccc gacttgtgaa ggtgcaggaa
      481 cacgaattgg tcggtgacga tcaatgtgcg ccccgcatca tggtttcgag ctcggcgtta
      541 ggagcgccga gctcacgttc ggcgcgcatc agcagcccga gcaattcagg gctcggcaga
      601 aaatcgcaga tcagatgtgt gcgtgcctcg gtcgtgtgcg cattccagac cccatgggtc
      661 gacgagttgt tcaacgccca cacctcgccc ggttgcatgc ggaaagcgag cccgccgcag
      721 tacatccagg cgtcggcatt ggtgacgacc ggtacgtgga tgcggatggt cttggcgaaa
      781 tagggcgcaa ggtcgatgtg cccggtgatc ctggcggccg ggggcaggcg cgccagcaga
      841 cagcgctgcg caggtgccgg gatcagctcc gcgatgattt cgcgcacata gggcatgtcg
      901 aagaggtgct cgcgatcttc gatgggtttt tcgccctcgg ctggggcgaa cccgcgcaga
      961 aaaatcgcct gcgcggcgcg atgcacgcca acccgaccgc cagtcccggc ccaggagcct
     1021 tcaggcagcg ccgcaacttc cgcctggagg cgcgccgcgt tcacctgcac gggcaggcgg
     1081 ctgcaggtgc cgatcaggcc cagcttgtcg agcggcggat gtccaggcag gtcgagcatc
     1141 gtctttccgc cagcgacgcc tcacgccgct ggcctttcag ttaaggatgc gcagcgttac
     1201 gcggcggttc tgttgccggc catcgtcggt gttgttgctg gcgatgggct gacgttctcc
     1261 gtaaccgcgc gacgtcatgg tgttgctcac accgccgtcc tgcaggtact tgagaaccgc
     1321 ctccgcgcgg cgcgctgaca ggtcgagatt gaaagcgtcc gaaccgcggc tgtcggtatg
     1381 accggcgact tcgacattga cctccggata acgcttgagc gtggcaatcg cattgttcag
     1441 cactggaatg ctctcgggca gcagggcggc gctgttcgtt tcgaacttca cgccctgcag
     1501 caacaattcc ttgttgaacg ggcaaccagt gttatcgacg cgttcgccct tgggcgtgtc
     1561 gggacacttg tccacgctgt cgacgatgcc atcgccgtca ccgtcgagct cacagccgcg
     1621 ctcgtccacc ttcgcgcccg cgggagtgtt cgggcactgg tcgcggctgt cgccaacgcc
     1681 gtcaccgtcg ctgtcgagtt cgcagccatt ggcgtccact ttcgctccgg cagtcgtgcc
     1741 agggcacttg tcgatgtcgt tggtgacgcc gtcgccgtcg tcatcctgcg gcagggggca
     1801 gccgctcgag tccacggccg tcccggcagg cgtaccgggg cacttgtcca ggttgtcgtt
     1861 gacgccgtcg ccatccgaat cgaggacgcg gcgtgagcca ccccaggaat actggaatcc
     1921 cagggcagcg acgtagtcga cagggtattc ctcggtggtg tcggtggtca gcgcgcggcg
     1981 cgcacctaag tcggcgcgca actgcatgac gctgccgtcg tcgcgatccg cgccgagggt
     2041 gatcagcagg ccgccgccat agagcccggt catgacttcg tcagcgacgc ccggcttgtg
     2101 gatggacttg gtttgcgcga ttcccagact caggaaggga tttacgcgcc cctcgcggta
     2161 gaacacgcga ttcagcgata gcccgtagcc ctgcaactcc agattggcgt cgcccacttg
     2221 cttgtgttcc gagccgtaca gggagacttc cacatcccac ttctcggtca tcgtgtaacc
     2281 gaaccccagc gtcacgccga cgtcgtcgtc ggcgagccgg ccgtcgtcta gccacaatgc
     2341 atttacccgc ggcgcgatga accagctgcc ttccgcagct ccaccgaatg ccgttcccgt
     2401 cgtaaatgcc gtggcgcaaa gagtggccgc gagcaccagg gtgccgattc ttttcatgga
     2461 ttctctccct agcaagtggc gcggatgata tcagcgcttt gcaagcgcgg gatgggcgac
     2521 gtgctggccc gggtgaaggc cgagacgcct ggcttcgcca ccggcgattt ccaataccgc
     2581 caccgccggt tcattgctgc ggatgatgtc gagcgaatgc ggcgttgcct gttcgacgat
     2641 ctgctggatg cggccgcgcg agtcgatgaa gagcatgtcg agtggaatgt aggtgttctt
     2701 catccacatg ctgattggct tagggctttc gtgcacgaac aacatgccgc gcaggtccgg
     2761 aagtgcccgc acgaacatga ggccctgcgc ctggcggctg gggctgtccg ccagccagac
     2821 atcgaacgtg agttttacct tcttgccatc gctgatctcg agcttgccgc gcggaaaggc
     2881 cgccaggtcc tcgaggggtc cattctgggc gagagcaggc gtgctcccag ccgcggccat
     2941 cgcggcggcg agcgccaggc accagcggcg cagacggtca gggcgattga gggtcaggcg
     3001 catggcgggc atcgtcctcg aattcgactc tgcctgcaaa gtcgaagctg gtgagcagaa
     3061 cccgttcgcc tttggagagg ctggtggcgg cccccaggca gaagccgcgc gcctcgacgc
     3121 gatagatagc gcggtctgca cccagtgact cgacgcgctg ctgggtcagt gaatccaccg
     3181 tgcacttgtc gtcgcccagc gtggcgaaaa tgcgatgttc gccttcaaag agaatcgtga
     3241 cgttggtgcg cagggtttct ccgcctcgtc cttcgacgac tccgccaatg ccgaagacca
     3301 ggcgaatgcg ccggccgtca ccgcgcaggg gcccggcgat gctgacacgg atgccgttgc
     3361 tctggttgtc ttttccgggg gggcgcgggc caccttcaca ttgcatctgc gcgtctttcc
     3421 agtcgaggtc caggtccacc gcgccgcgaa tgcgcgcccg caggtagcca ttgccagccg
     3481 cgaagcaacc ggtcttctcg gcggggtcgg taactgggct tgcaggcgga gcggcgcaag
     3541 cggtatgcgc gaacgccgcg ccgagtgcag caattaatgc tgcggcagat gcgcgatgag
     3601 gcatgcgcct acggtagcac gagcatcgat ttcgccagca ctgcatgcgc cttcgtaacg
     3661 ccacgcgcga ggtgcagcgt cgccgtgagc agcaggatgc cgacgatccc gagcaggacg
     3721 aggccaccgg gtgtgccgag ccatgccgga ttcatgtcga cgaccacgcc gttgtgcacc
     3781 gggtgcactc cgtgcgcgaa gaccgactga tcgctgaacg cgccgagtgc acgcaggcct
     3841 tcgatgacgg gcgcggcaat cagcgcgagt ccaaaggata gtccgaccac ggccatcatg
     3901 aagtacgcga cgccgagcgg cagcatcagc aggtcgtacg cgagcgtggt ccaggttcgc
     3961 gagtccgtga ccatcgcctt cacgcggtcc cagaatcccg agggtgcgcc gggatgcaag
     4021 ggccgccgcg gcatgcgcac accgctcacc gcttccacca gccggcattc caccagcgcg
     4081 agcgcgcgcg ttgcgccgat gaatgcgagg aagaacggga cgccgatgat caggaacgcg
     4141 agaccggccg acagcgacag tccagtcacc gccaccgtga aatacaggat gccggttgca
     4201 aggctgaaca tcatgaacag gaggccggca taggcgcgcg gatccaggaa gatgccgaag
     4261 aaacgcccca gcatgcttgc acgcggcggc gcgcgtggcg tgcgaagggc ggcgttcaat
     4321 ttcgtttcgg tgtcgcgata ggccgccgcg acttcctccg gagcgccgta ggtcgatgca
     4381 atcaactcga gcacatcggc ctcggacttg tccgggtggg aggcgatctc ggcgcgcaga
     4441 tattcctcgg cgtcgtataa cgcgtcctgg atgagcgcga tgtcctggtt ggcgagcgcg
     4501 gcgcgcagct ggcgcaggta gtcctcgacc gaacgagggg cgttgtcagt cattggaagc
     4561 tccggattcg acgaagcggt ccacgaactc gcgcgcgtca cgccagattc cacgccaact
     4621 gccgagcacg gtgcggcctt cgtcggtgat cctgtagtaa cggcgcgggg gaccggaata
     4681 cgaaggcacg atgcggctgc tcagcaaacc gtttgccgac agcgcgcgca gtacggggta
     4741 gaccgtgcct tctttgaaga gcaactcgcc ttcgttggcg cgttgcaggc gtttgacgat
     4801 gtcgtagcca tagaggtcct cttcgcttcg ctcgagcacc gcgaggagca caagcgagac
     4861 gagacctccg ttgagatcct tctgaaactt cttggcgtgg ctatcgtcca ttggccagta
     4921 ctgtaatgtt gctagtacta gttagcaagc agtattttca ttcgtaagta accgcgccat
     4981 ttggccctgg ccggcttccg gggctgacct atactcggga aaaatcgagg gacccatggc
     5041 aaaacgccca gcacgtgcaa catccggtcg ttacgtcagc cggctcacga gcggaacgct
     5101 cgcggtcatc atggcgggtg gaaggggcga acggctgaag ggtctcaccg aacaccgcgc
     5161 caagcccgcg acgcctttcg gcggcaaatt ccggatcatc gatttcgtgc tgtcgaactg
     5221 cgtgaattcg ggcatacgcc agatcacggt cctcacccag tacaaagcgc aatcgctgat
     5281 acagcacatt catcgtggct ggggctacat gcgaggcgag ttcggcgaat tcgtcgaagt
     5341 gatcccggcg cagcagaaga ccggcacaca ctggtaccgc ggtacgtcgg acgcggtgta
     5401 ccagaacatc gacgtcatgc attcgcatcg gccgaaacac gtgctggtgc tggccggcga
     5461 ccacatctac aagatggact acggtccgat gatcgcctac cacgtggaga agggcgccga
     5521 catcaccgtg ggtgtcgtcg aggtgccgat cgtcgaggcc aaggagttcg gcgtcctcac
     5581 cgtcaccgag tggaatcgcg tgacccgctt cgtcgagaag tcgccgaatc cggatcccat
     5641 gccaggccgg accgacgtcg ccctggcctc catgggcatc tatgttttca acccggagct
     5701 gctcgaaaag ctgctcatcg aagatgccgc cgacgagaag tcgaagcacg atttcggcaa
     5761 ggacatcatt ccgaagtcca tcgagaagct gcaggtcttt gcatatcctt ttcgggacgt
     5821 aaagacacgt gcacagtcct attggcgcga tgttggaaca atcgacgcgt tttacgaggc
     5881 caatctcgag ctcgtgtggg tcgcgcccga actcaatatc tatgacgagg actggccgat
     5941 ctggacgtat caggcccaga ccccgccggc gaaattcgta ctcgacgaag atggccggcg
     6001 cggtgtggcc atcaactcgc tggtctcagg cggcgtcatc atctcgggcg cgacggtgcg
     6061 cgagtcgctg ctgttctccg gggttcgggt cgaggaacgc tcagtcgtcg agcgatccgt
     6121 gatcctgccg gacgtgcgca ttggccagaa ttgccagatc cgcaacgcca tcgtcgacgc
     6181 gggctgcgaa atcgccagcg acatgcggat cggtttcgat cgcgccgaag acgccaggcg
     6241 attcctggtg acggaaaagg gcgtggtgct ggttacgccg gacatgttgt cgcatgcgtg
     6301 agcaacgcgg cgcgcgtcgc ctggctctgg tcgctctcgc ttccctgacg ctgttgtcat
     6361 tagtcggggg tggctgcgcg atggtgccgc aactggaggc gccaaagttg tcggtggtct
     6421 ccctgaagat gcagggcggt gacatctttt cgcagcgcgt gctggtgcgc atgcgggtga
     6481 tcaatccgaa ttcgcgcgag ctgccgatca agggcctcac ctacagcatc gaggtgaacg
     6541 acgccgaact tggcaatggg gccaccgcgg cgccgttcac cgtgcccgcc atgggtgaag
     6601 ccgagttcga catgcagatc acggcgaatc tcgccggtgc gctcgccaaa ttattgtccc
     6661 ggcgcagttc gtccgaggcg ctcgagtacc ggttgcgcgg gaacgtaagc ctatcgagcg
     6721 gctttctgcg ccggatcccg tttgatgagc gcggcagcgt caagctgaag tagctccgcg
     6781 aacaacttca ggtattcgtt ggcctgcgtt tcccaggaga aatcgcagcg catggcgttc
     6841 tgcacgatgc gttcccacag cgacggctgc gcgtaccagc tcatcgccgt gtccagcgcc
     6901 caggtcatgg cggtcgcgtc gaaatcgttg aatacgatgc ccgtgccttc cccggtggcc
     6961 ggatcgaaat gggtgaccga atcggcgagc ccgcctgtcc ggcgcacgat gggtacggtt
     7021 ccataccgca gcgaatacat ctgattgagc ccgcagggtt cgtattgcga gggcatgagg
     7081 aaggcatcgc tggcgccttc gatccagtgc gccagagctt cgtcataacc gccgaagaac
     7141 cacgcgcggc cgcgatactt cgcggcgaga cccgcgaaaa agtcttcgta taggcgatcg
     7201 ccggtaccga cgacggccag tcggcagtca ttgttctgca gcagcgccgg cagcgtgtct
     7261 gtcagcaggt cgaagccctt ttgagatgcg agccggctca cgatgccgaa cagcggcacg
     7321 cggccacgcg tcggcaccat gccctgccgc agcaggaaat tgcgtttgag ctcggccttc
     7381 ggcgcgatgt ttcccgggcc gaagtggatc ggcaggtgct tgtcgtgcgc cggatcccac
     7441 tcgtcgtagt cgacgccatt gaggattccg tgcaccgcgc tgccacgcga ggccagcgcc
     7501 tgttccatcc ccatgccgta gcgcggcgtg gtgatttcgc gggcgtaagt cgggctcacc
     7561 gtggttaccg cgtcggcgtg caggatgccg tgcttgagcg cgttgatgtg cccggccctg
     7621 aggtcgtcct ggtgcagtag ataggtgtcg gcgccgagac cgaggtccgc gatatcgagc
     7681 gcgctgaacg caccctggta gccgatgttg tggatggtca tcacggactt cgtgccggcg
     7741 aagaccgggt cgctcttgta attcgagcgc agccagagcg gcgcgaacgc cgtgtgccag
     7801 tcgtgcgaat gcacgatgtg cgggcggaaa ttcatgcgcc ggcaacactc gagcgcggcg
     7861 cgagtcagga ccaggaagcg ccgatgttcg tcggcgtcgc cggtgtagag cttcggacgc
     7921 gcgtacaacg cgtcggactc gaccaggtaa accggagagg cggatcccgg cagggtggcg
     7981 cgccagatgt cgacgaggta ctcgtggttg ccggccagga tgcgcaggcc ctgcagcgtt
     8041 tcgacgcgct tggcgtccag cttgtcgcgc ctgacctgcg aataaaacgg catgaacagc
     8101 cgcacgtcat gcccggcgac tgtcagctgt cgcgaaagcg cgctcgcgac gtcagcaagg
     8161 ccgccgctct tggcgagcgg gaccacttcg gatgcgatgg cgcaaatgga gagtttcacg
     8221 gcggcgggca ttctagcgtc tcattcccgc tggtagcatt aggccaggtc tcaaagtcca
     8281 atgcgccgcg tactcacatt cctcacagcc atcgtgctgc tgctggtgtc tcttggcgtc
     8341 ggcgtgttca cggccgacct gccgttctgg cggcgcgcgt tgcaattgcc actccccgcc
     8401 gacggtgcct acctacccgt ggccagaatc ggcacgccgg agtcgccgcc cccgcatccg
     8461 ccacacaggg aacccgcgaa tccgcccgcg cctccaatcg atgccctggt ggtcgaggaa
     8521 gcggccaatc gcgcgggcaa ctccggatcg cgagccttgt tagtcctgta tcgcggatcg
     8581 ctcgccgtcg agcgttattt cctcactgac gacgcgcaca cgctgctgcc ggctgcactg
     8641 gtggcgcgcc caatcgcggc catggcgatt ggcatagcgc gcgccgacgg gaaaatcgcc
     8701 agcctcgacg atcccgtcgc gcgttacctt cccgagtggg acgacgaggc gcgtggccgc
     8761 atcacgctgc gccagcttct cgaagagacc agcgggctcg agaccggcgg cgatacacgc
     8821 ggcctgctgc atcgctcgcc gtggcgcgaa ccggcgtcac tgccggtctt cgccacgtcg
     8881 cgcggcgtac gcatgttgtt aggcaacgat ttccagtcga gcgcgctcgg cttccggctc
     8941 gaacacgagc cgggcggttt ctacaacctg tcacccgcca atacccagct cgccgcggtc
     9001 atcatcgagc gcgtcaccgg cgagccctac gaaaatttcg tggacgagag gctgtggcgg
     9061 ccggtgggcg caggcctcgc cgaaatgcag ctcgatcgcc gcgcgggcat gcccgcggcg
     9121 cattgctgct ggcgtgcgac ggcgcgcgac atgatgcgca tcctcagcct gctcggcacc
     9181 gatggcgttc acgatggacg tgcggtcctg cccgccggct gggcgagcga gatggcgcgg
     9241 gcttcgcgcg tcagcgccgg aaccggcatg caggtaacgc ggctcagcat cgatggcgcc
     9301 gaagcgctcg aggtcaggga cgaggccggc agcgcgttct gggtcattcc tcagcggcaa
     9361 ctcgcgatcc tcaacatcac gaacccgggc ggcagcgcgg tcacggactt gccggcgatg
     9421 ttgctgaagg ggatcgaggc gccggcgccg tgaacggcgc aaggatcggg tcgaagcgaa
     9481 agagcgtggc gcgcagaatt cgagccatgg atatgaaagt cacatcgatc tcagacgcat
     9541 tcgtcgacag ccgtgagttc gcgcgaatga cccgtgcgat cgaattcatc gaacgcgagt
     9601 accagcgcca gccgaagctg gcggaaatcg cccggttcgt cgggctctcc gaattccatt
     9661 tcaaccggct gttccgccgc tggacgggat tgaccccaaa gcagtacctg gccgaagtca
     9721 ccagccgcgc agctggcacc gcgctgcgca acgagccatc ggtgctcgat gccgctcact
     9781 ctgtaggact ttcgggaggc ggtcggttgc acgacctgac ggtgacgctg gaggccatga
     9841 cgcccggcga gcttcgcgcc ggcggctccg gcgtgaccat ccgccacgga attgcgaaca
     9901 cgccatttgg cacggcgttt tttgccgaga cgccgcgcgg tttgtgtcgc ctggcattca
     9961 tcgacggacc gggcgatgga aaagagctga agctcctgcg cggtcagtgg ccgaaggcga
    10021 agttcgtgcg cgacgacgag cgcgctacga ctttggtcga gaccatctgg ggtcacaaaa
    10081 ccgtaaccct gccgctggcc gtgtgcggta ccaatttcca ggtgcaggta tggcgtgcct
    10141 tgctcgaact agatgccacg gagtcggtct catacagcgc gctggcacga cgactcggat
    10201 tgccggatgg cgcgcgtgct gtgggcaacg cagtaggtgc gaatccgatt gcctgggtca
    10261 tcccgtgtca tcgcgtgctg cgcgcaggcg gacaactcgg tggatatcgc tggggtgcgg
    10321 cccgcaaaca gatgattcgc cgctgggaag aaaggcgtac cggggtctca taacattatg
    10381 attcccctcg tgtgtcggga tgttagcggt gccatacttg ccctccgcca acgaacgatt
    10441 cgacaccgca cacgatgtca gagaagcgca gcctcaccac ccccatcagc attgccgttg
    10501 cagtggtctg cgccgcgtgg gctatctacg cgacgcagca caaacccgcg gccgggtcgg
    10561 gcggcccgcc ggccgccgcg gggcgcaaca atgcgccggc attcgtaacc ctcgcgccgg
    10621 tgcgcaccga gcgcatcagt cagaagctcg aggcgctcgg caacgcgcgc gcgaatgagt
    10681 cggtcgacat cagcacgaaa ctctcgaaca tcgtcaccgc ggtgacgttt cgcgacggcg
    10741 agcgcgtgaa gcgcggacag gtgctcgtgc agctcgacaa cgcgcaagca cgcgccgatg
    10801 tcgccgcggc gcaggccgcg gtcaccgaaa gcgagagcct gtacaaccgc agccgcgagc
    10861 tgctcaacac gcaggcgctg tccaagtcga gcttcgatca actcgaagcc accttgaagg
    10921 cgaatcgcgc ccggctggga gctgcgtcgg cgcggctcga agacaccgtg attcgcgcgc
    10981 cgttctcggg ccgagtaggt ttgcgtcgcg tcagcgtcgg cacactcatc agccccggcg
    11041 atgtcatcac cacgctggat gacaccagcg tcatcaagct cgatttcgcg gtaccggaga
    11101 attttctcgc cagccttcgc gaggggttga cgatccgcgc cactgctccg gcgtttccgg
    11161 gtcgcacttt cgcaggctcg gtctccagca tcgattcgcg tgtggatatc gctacgcgct
    11221 cggtgacggt ccgtgccttg ttaaccaacg aagacggcgc gctcaagccc ggcatgtttc
    11281 tcaacgtaag cctggcgaac gacgaacgcg aagcgctggt cattcccgaa gaggcgctca
    11341 cgcccgaagc cgaacggcaa ttcgtgttcg tggtgacgga cgggaaagtt tcgcgtcgcg
    11401 aagtgcgcat cggtggccgc cggcccggca gcgtcgagat tctcgccggg gttgttgccg
    11461 gagagcgcgt gatcgtggaa ggcacgcaga aagtgcgcga tggtgcaatg gtgcaggcgg
    11521 ccgagcgcgc atccgaggcc gaccccgccg gccccggcgc cgtaagcgag tcgcacgagg
    11581 ccaacgcaag ccccgcgaga atcgcgcaat gatgatctcc gacttatcgg tgcggcgccc
    11641 cgtcttcgcg acggtgatgt cgttgctgtt gttcattctc ggtgtaggcg ccgtcacgcg
    11701 cctgtcgttg cgcgaatttc ccgacgtcga tcgtccggtg gtgaacatcg agacgcgtta
    11761 tcgcggcgcg tcgtccgacg tagtcgaatc gcgcatcacc caggtcatcg agaacgaaat
    11821 ctccggaatc gaaggcgtcg aacgcctgaa ttccggcagt cgcgacgagc gatcgcagat
    11881 caacgtcgag ttctcactgg atcgtgatct cgacagcgca gcgaacgatg tgcgcgaaag
    11941 gctgtcgcgc gtcgccggcc gattgccgct cgaggccgat ccgtcgcaga tcaccaaggt
    12001 ggacagcggc accgacccga tcgtgtggat caacgtgacg agcacgcagc gaagtattct
    12061 cgagctcacc gactacatgg agcgtttcct cgtcgatcag ttctcgacgc tcgagggtgt
    12121 tgcctcggtg cgcgtgaacg gcggccggcg atacgcgatg cgcgtgtggc tgtcgcgaga
    12181 gaacctcgcg tcgcgacaac tcacggtaaa cgacgtcgag aatgcgttgc tgcgcgagaa
    12241 cgtggaactg cctgctggcc gtctcgaatc cacggaaagg gagttcacgc ttcgcacgga
    12301 caccaacctg cgtaccgaag cggacttccg gaatctcgtc gttggccgcg gcgcggacgg
    12361 ttatctcgtg cgcttgagcg aggttgccga ggtgcgcctc gcttccgaag acgatcgcag
    12421 catttcgcgc tcgaatggcg tgattggcat gtcgatggcc gtcttgccgc aatcgaaagc
    12481 caacgtgctc gccacttcgg cggccgtcaa gaagcaactc gcgtcgatcc agtccacctt
    12541 gccgaaggac atcgtcgctg aaatcaacat cgacaacggc atgttcatcg ccgcatcact
    12601 caagaacgtc gtggtggcgc tgagtgagac gctggtcctg gtgctcgtcg tcatcttcgt
    12661 attcctcggg acgctgcgag ccacgctgat cccggccgtc accatcccgg tatccatcgt
    12721 cgccgcggcc atcgtgatgc tcgcgctcga ctattccatc aacacgctga cattgttagg
    12781 agccgtgctc gccatcggca tggtggtcga cgatgccatc gtggtgctcg agaacatcgt
    12841 gcggcgcatc gaaggcgggg agcccgcggt gctcgcgtcc atcaatggct ccagggaaat
    12901 cggtttcgcg gtcattgcaa ccacactggt gctcatggcc gttttcgtac ccgtttcgtt
    12961 catgaccggc gataccgggc gcttgttcgg agaattcggc ctcacggtcg ccgcggcggt
    13021 gggattctct gcattcgtcg cgctcaccat gacgccgatg ttgacctcga agctcttcgc
    13081 cggtggcatg ccgaagtcgc gcatgaccgg cggcatcgac cggatgttcc gcaacctcga
    13141 cgaaaaatat gcgcgcggcc tgcgcagcgc gctctcaggc aagcggccgc tctggatcgt
    13201 cagcggttcc ctcgcgctgt tcggtgtggt cgcgacgttg atgatccagg ggttcccatt
    13261 ctcggccctg aggctgccga gcgagctggc acccgaagag gatcgcgcct tcgtgcgcat
    13321 tttcgttggg gcccctgaag gggcgagcct gtcctacctc gaccggcagc tacgccaggt
    13381 cgaggaaatt gcgcaggatg aagtggagcg cggcaatgca aaacgcgtga tcgttcgcac
    13441 cggcggattc ggccgcgccg cggagaccaa taccggcttc gttgctttgc cgctcaacct
    13501 ttgggacgag cgtgacgaca gcgcggggct catagccgac aggttgcgcc agcgaaccca
    13561 gaacatcacc ggcgcgcgag tgaacgtgaa ccaggccggc agtccgctga cgcgcaacag
    13621 cacccgcccg cttgccatag cactcggcgg cggcgattac gccgaaatcg tgaaatggcg
    13681 cgacaagatc atgaatcgtg tcgaggcgga gaatccccgg atcctcgatc tcgaatccga
    13741 ttatctggag cgcaagccac gcgtcatcat tcagatcgac cggaacaaag cggccgatct
    13801 cggtgtctcg ctgcagtcag tgggccgcac gctcgagaca atgctgggct cgcgcatcgt
    13861 caccacgttc gagcgttctg gcgaggaata tgacgtcatc cttcaggcgc gcgacgaaca
    13921 acgcgccacc gtcggcgacc tcgataatct gtacgtccgt tcggacaagg acggtgacct
    13981 gctgccgctc tcggcgctgg ttcgcatcga ggagcgcgcc gggccaaacg agctgcggcg
    14041 caccgaccga atgcgcgcga tcgagctcgc ggggtctctt gcgccgggct acgccctcgg
    14101 cgacgccgtg aagtacatgg agcaggtgat acgcgaggaa gctcccgagg cgcagatcat
    14161 ctacaacggc cagacctatc agttgaaaca atccggcagc acgttgtgga tcactttcgc
    14221 gttcgcgctg ctcgtggtgt atctggtact ggctgcccag ttcgagagct ttatccatcc
    14281 ggtggtgatc ctggtgaccg tgccgctcgc gatcaccggc gcgcttatcg gattgtggtt
    14341 gttcaattcc acgatcaaca tcttcagtca gattggctgc atcatgctga tcggcatcgc
    14401 ctgtaagaac ggaatcctca tcgtcgagtt ggccaatcag ttgcgcgatc gcggtgtgga
    14461 gttcgttgac gccgtcatcc aggccgcatc ggtgcgattg cgcccggtcc tcatgaccag
    14521 cctgtgcacg gccttcggcg ccgtgccgct catgctcgcg cacggcgcgg gcgctgaaag
    14581 ccgcgaatcg atcggcgcga cggttttctt cgggaccgtg gtctcgctcg ggctcactct
    14641 ctacgtcgtg ccatcgctgt atctgctgat cgcacgcaat acccgctcgc cggaacacat
    14701 gacgcggctc atcgatgcgc tgctgggtgg ccgtcccgca cgtggcacgc cggcaacagc
    14761 ggaatccgcg gcggcgccat cggcggagcg cccgtaaagc caaaacgcca cagcgcgttt
    14821 gacataaatg tgccattcgg cagccaaagc ggccggcgct gtcaacccaa acctgaccga
    14881 ttaagtcttt gatcctgatt ggattagggc tcccctggag cggctggaac gagggtcgct
    14941 ggtagactgg cgcccgaccc gttttccccg tccgtgacga tctccatggc ccagtccccg
    15001 tacaaacagc tcgagcagga atggaaacgc ctgcatgcat tccgtggtgc catggccatc
    15061 cttcgatggg acgcagcggt aatgatgccg cgcggcagcg ccgatgttcg cggcgagcaa
    15121 cttgcagcgc tcgagaccga ataccacgcg ctggtcaccg cgcccaggat cacgcgcctg
    15181 ctcgaccgcg cccaggccaa taccggcggt ctggaggact ggcagatttc caacctgcgc
    15241 gagatgcgcc gccagcgtga cttcgcaatt gcgactcccg tggcgctggt ttcgcgcctg
    15301 gcaaaggccg cgtccaaggc cgaggccaag tggctgcatg cgcgcgagac cggcaagttc
    15361 gaagacttcg cgccgcacct cgaagaagtg gtgcacctgg tgcgcgacaa ggcgcagctg
    15421 ctgggccagg cacgcaacct cgcgccgtat gatgcgctgg tcgatgagtt cagccccggg
    15481 ctcacctccg gcgagatcga cgcgatgttc aagtcgctgg cgcgccgcct cccgggcctg
    15541 atccgcgaag tgctcgtgcg ccaggagcag cgaccggtac ttcccatcgt cggcaagttc
    15601 agcgccggca agcagcgcgc attggcactc gagatcatga aggccgtggg gttcccgttc
    15661 gatcgcgggc gcctcgacga aagcgaacac ccgttcaccg aaggtgtccc gggcgatgtg
    15721 cgcgtcacca cgcgcttcga taccaccgag ccgttctccg gaatgctcgg cgcgctgcac
    15781 gaaaccggcc acgcgatgta cgacgtgggc ctgccggcgg aatattccga ccagcccgtg
    15841 ggccgtgatc gcggcatggc cctggaagag agtcagtcgc tgctcatgga aatgatcgtg
    15901 tgtcgcagcc gtggcttcgt ccgctatctc aagccactgc tggaaaaaca ttttggagtt
    15961 tcgggtcctg aatggtcgga ggaaaacctc tacgcccacc tgacgcgcgt gaaacgcggc
    16021 ccgattcgcg tcgatgccga cgagctcact tacacgctgc atatccagct gcgttatgaa
    16081 ctcgaaaacc agctgctgtc cggcgaactg gcggtgcgcg acctgcgcga tgcctggaat
    16141 tccagcatgg agcagcggct cggcgtgaaa ccgcagaacg acctcgaagg cgtgctgcag
    16201 gacgtgcact gggccgtggg atcgttcggc tacttccctt cctatgggct cggcgcggtc
    16261 atggccgcgc agttctacga ggcgttgcac gaagcggtgc cggacgtcga gacgcagatc
    16321 gcgcgcggcg atttcggcgg cctcatgggc tggctgcgcg aaaacgtgca taggcacggc
    16381 gcgcgggtca ccacgcagga gttgctcaag ctgtccaccg gcaagccgct gtcagccgtc
    16441 tcggcactgc gctacctcga agcgaaatat ctcgaacccg agtcggtagt cggcagcgcc
    16501 gcggcatgag tcgcgggcgc gtcgccacat gagccagtgg cggggctggc tggtcggcgc
    16561 gatcagtaca gtattttttc tggcggcgct gctcgtgccg ccgatgccgc agccgctgtc
    16621 gtatcacgtg ttcgccgact gccgcagttt cttcggcgtc gtcaatttct tcaacgtggt
    16681 atccaacttc ccgttcctga tcggcggcgt tctcggcctg cagctcatct ggagcggccg
    16741 cgtgacgttc atcgatccgc gggagcagct gccgtatatc gtgttttttc tcggcgcgct
    16801 gctcacgtgt ttcggttcgg cgtactacca cggtgcccct gacaacgcgc gactggtctg
    16861 ggatcgcctg ccgatgaccc tggggttcgc cggcctggtg gccgcgacca ttgcggagcg
    16921 cgtggattcg aagctcggcc ggtggtcgct gtggccgctg ctgctcctcg gcgtgttcac
    16981 cgtcgtctat tggtacggga gcgagcttcg cggctcgggc aacatcatcc cgtacggcgc
    17041 ataccaggcc tggagcgtgc tgatcatcgt cttgctgttg ctggcctatc cggcgcggct
    17101 ttacagccac ggcgggctgc tcggctgggc ggctgcgtgg tacggcctgg ccaaggtgtt
    17161 cgaaactttc gacctgcaga tctaccggct gctcggttcc acgctgagcg gccacacgat
    17221 caaacatgtg ctggcgtctt tcgcggtctt cgccatcgtg cgccagttgc gggtgcggca
    17281 tagaattccc cgcccatgag cgcccagccg gaaaatatcc tcgattcccc agaggggctc
    17341 gaaacttcac ccgagtggcc ttcgcagacc gcgaagaagc tcggcgcaca cctgcgcggc
    17401 ctcaccggca aggccatcga ggattaccgc atgatcgccg acggcgatcg cgtcatggtg
    17461 tgcctgtcgg gcggcaagga ctcctacact ttgttagacg tgctgctgtc cctgaagcgg
    17521 tcggcgccca tagatttcga cctcatcgcc gtcaacctcg accagaagca accggacttc
    17581 ccggccgagg tgctgcccaa ctacctgcgg ggcctgggtg tgcctttcca cgtgatcgag
    17641 caggacacct atagcgtggt caagcgcgtc atcccggaag gcaagaccat gtgcgggttg
    17701 tgctcgcgca tgcgtcgcgg cgccttgtac cgctacgcgg cggaaaacgg catcaccaaa
    17761 atcgcgctcg ggcaccaccg tgatgacatc gtcgagacgt tgttcctcaa catgtttcac
    17821 ggcggccgcc tcaaggccat ggcgccgaag ctcaagtcgg acgacggccg ccacatagtc
    17881 attcgcccgc tggcgtatgt cgccgagtcg ttgatcgaga gatatgcgcg tgcgcggcag
    17941 ttccccatca ttccctgcaa gctgtgcggg tcgcaggaca acgcgcagcg caaggaggtc
    18001 aagcgcatgc tggccgaatg ggagcggcag tatcccggtc gcaccgagtc gatcttctcc
    18061 gcgctgcgca acgtggaagc cgctcacctg gccgatccgc gggctttcga ttttccgggg
    18121 ctggatgccg aacgcaccca aatccctgaa taaacccatc ggcgagtcgt actgggtgat
    18181 cgaagatcgc ctgctggccg gaagatatcc gggtggcaag acgcccaagg aagccgagcg
    18241 ccgcgtggcc tcgctgatcg acgcgggctt cgacgccttc atcgacctga ccgagccggg
    18301 cgaacttccg ccttacgacc tctatctacc gggcagcgcg ctgtatgtgc gcaaaccgat
    18361 ccccgatcac ggagtgcccg gcgcgcccga atacatggcc ggaatcctgt cggagctcga
    18421 cgcggcgctc gcctccggcc ggcgcgtgta tgtgcactgt cgcgcaggaa ttggccgcac
    18481 cggcacggtg atcgcctgcc atctcatcga acaggggctc agccccgatg ccgcgctgat
    18541 ccggctcaac gagctgtggc agggcaacga cctctcggcc acgtggtccg acgtccccga
    18601 gaccgacgaa cagcacgaat acgtccgcgc ctggacgccc gcggccgatc ccacgaagca
    18661 gcccgatgtc atggatgcgg cgcgcacgct gcgcgagcga ttccaggggg ccctggtcgg
    18721 gctggccgtc ggagacgcac ttgccgcgca cacgcagttc cgcaaaccgg gaagcttcgc
    18781 cgccgtgggg gacctgctgg gcgggggtcc attcgaccag ccgcgcggcg cctggaccga
    18841 cgacaccgcc atggcgctgc tgctggccga aagcctgctt gcacgcgagg gcttcgacgc
    18901 gcgcgaccag gtcgaacgct acgtgcgctg gcagcgcgag ggctacggaa gcgcgaccgg
    18961 gcagtgtgtg ggcatttccg ccaatgtggc ccggtcgctg gctaccgcgc agtacaagcg
    19021 ccagccgttc gccggttcgc atgacccggg gcaactcgac aaggacccgt tgtcgcgcat
    19081 cgcgccggtg gcgatgtact tctttgccga cgcttcgacc gcggtagcca aggcagccga
    19141 atcggcccgg gtcaccgcac aggcgcccat ggttctcgat tgcgtgcggc tgctggccgc
    19201 catggttcat ctggcgcttt cgggtcgcga caaggccacg ctcctgcacc cgccacggga
    19261 tctgtgggcg acggcaagca cccggccgga ggttctcgcc ctctacgacg gggcctacgc
    19321 gcaccgcatg cctccggaca tcaccggcgg tggtggaatc ttccaggcgc tcgaggcagc
    19381 cctctgggct ttccaccgca cggaatcctt ccgcgaagga gccttgctcg ccgccaacct
    19441 cggacgcgac tcggatgtgg tggccgcggc ctacggtcag atcgccggtg cctatcacgg
    19501 tgtgagtgcg attccgggaa tctggcgtaa cagcctgatg aagcaagaag tggtcatcga
    19561 cagcgcggac cggcttctca ctcatgcgct cgtgaccctc ggatcgtgat tccgcaagtc
    19621 aatcgtctcg cttaaagtgc tggccatgcc ctccacgcgc acctcccgcg gaagccttgc
    19681 caagcgctcg tggacgcggc tgtctgacga acagctgttg ggcctgcggt tctgcgatct
    19741 gaaactcaag atcgagcgtt caccggtcgc caagcgcgtg cgccggctct accgcgagct
    19801 cgacaagcgg cagatttcct ttcgacctca tgtgtggctc tcggaagaat ggttttctcc
    19861 cgatggtgtg cccggcatcg ccgtgccgtt ctacctcgcg catccgcgcc tcgaacggct
    19921 cgagcgccgc atgatgcgca acgtggaggg aggcagtgcc gaaagcgcca tgcgcatcct
    19981 gcgccacgag gcgggtcatg ccatcgacac tgcgtaccga ctccgccgcc tcaaacgctg
    20041 gcgcgagatt ttcgggcccg cttccctgcc gtatcccgac acctatcgcg cgcgccccgg
    20101 cagccgccgc tacgtgcagc acctcggcga gtggtacgcg caggcgcacc cttgcgagga
    20161 cttcgcggaa accttcgcgg tctggctcaa gcccaattct tcgtggcggc gcacgtacgc
    20221 gcagtggccg gcgttccaca aactcgaatt cgtcgacgag ctgctggaga aggtccgcgg
    20281 cacgcgtgcc ccggtgcgca atcgcgaagt cgtcgagccg ctgcgcgaga acacgcacac
    20341 actcgccgat cactatcgcc gcaagctgcg tcgctacagc atgtaccggc gcacggtcac
    20401 cgatcatctc ctcgaacgcg tgttcaccgc cgagcgtctt cggcgcgcgt cgcgagcgag
    20461 caccttcctg cgcgcgcaca gcctgcacct ggtgaatgcc acgatgcgcg agctgcatgc
    20521 cgagcgatac agcgtggagc agatcctgcg catcgtcgtc gagcgggccg acaagctcgg
    20581 cgtgtgggtg cgcggcagcc ggcgcgacgc gctgcgccac gcgcgctgga tgctggtcta
    20641 cctgacgcgt ctgtacgcgc agggtgaaag cccgcgtctt tcactatgaa gcgactgcgg
    20701 acgctggtcg tcgttcattc cacgctcgta ccacccgact cgctcgaagg gcacagcgaa
    20761 aaagaaatcg aagagtggcg taccgaattc gacgtggtgt cgactctgaa gaagtccggc
    20821 cacgaagtgc gctgcctcgg catctccgac agcgtcgaag agctgcgcag cgtgatcacc
    20881 gaatggaaac ccgagatggt gttcaacctg ctcgaggaat tcgacggcat cgtcacctac
    20941 gaccagcacg tggtcgcgtt cctcgagctg ctgcgccagc cgtacacggg ctgcaatccg
    21001 cgcggcctgc tgctctcgcg cgacaagccg ctgtgcaagc agttgctttc gtatcaccgc
    21061 atttcgtcgc cgcagttcgc cgtgttccgc cgcggcatca agctgcgcct gccgcgcaag
    21121 atcaaatatc cgctgttcgt gaagtccacc accgaagacg cgtcgctcgg catctcccag
    21181 gcctccatcg tcaccgacaa ggcgcaactc aaacagcgaa tccagttcgt acacgaccag
    21241 gtgcagaccg atgcgctcgt cgaggagtac atcgaggggc gcgagctgta cgtgggcgtg
    21301 atgggcaacg accggctcac gcggttgccg gtgtgggaga tgaagttcgg gtcgttggcc
    21361 gagacttccg cgggcatcgc cacgcgccgc gtgaagtggg acaagaagta ccagaagaaa
    21421 catggcatcg acacgcacga agcgaaagat cttccgcccg ccgtggtggc gcggctcgac
    21481 aaactctcgc gccgtgtatt tcgctcgctg ggtctttccg gatgcgcgcg catcgactac
    21541 cgcatgaacg ccgaggggca gatctacgtc atcgaggcaa accccaatcc caacctcgcg
    21601 gcggacgagg attttgcgca gtcggcgctg gcggccggcg tgagttaccc ggagctgctc
    21661 gagaaactgc ttacgctcgg cgccggttat caggcagagt ggcgcgcgta ctacggctga
    21721 ttttcgcggg aatttcttat gaagtattgg cctatgaaga actggtcgca tcgcgccgtc
    21781 gtcggcacgt ttctcctggc actcgttccc gcactctcgg gctgcatggt cagcgcagcg
    21841 gaaatgcagg acaacatcac gaagttcagg gcgtccacct cgggccaata caaaagtgag
    21901 gccggtgcaa agttatacat cgcgccggtg cgggcccgca tggtcaccga cgaagcgctc
    21961 tacctcgagc gccacgatcc gaacggcacc atcgtcgggc ggctgctcaa tgttgaagtg
    22021 gccgaagacg gcaagacgat cgtattgcgt gcgctcgtgt tcacgcagga gggccagtgg
    22081 cgcaacctgc gtgaaaatcc cgagctgttc accgcactgc tgccaaaaga cgtgcggcct
    22141 gcgggcaagt gcgacatcaa gccgtcgaaa gaccgggatt cgctcaccta ttcgtgcggc
    22201 ggatcgccgg tcgagaactt cacgcgcgtc accgaatagc gaaaggcgga gcgccccgct
    22261 atttcgtgtg tgagtccagc cagtcgagca cggtgccgta ccactgaatg ctgttgttcg
    22321 gtttcagcac ccagtgattc tcatcgggaa agtacagcag cctgctttcg atgcccaggc
    22381 gctgcagcgc cgtgaacgtg gcgatcccct gagtgtcggg cacgcggaag tcctgctcgc
    22441 catggatgac aagcatgggc gtgcgccact gcttcacgtg gttggccggg ttgaaccgct
    22501 cgtgtatctc gggcgtctgg tagtaggggc cgccgttctc ccattccgta aaccacaatt
    22561 cttcggtggt gtagtacatc gaccgcgtgt cgaagatgcc gtcgtgattg acgatgcagc
    22621 ggaaaccgtc gggccagttg ccggcgatcc agttctgcat gtaaccgccg tacgacgccc
    22681 ccagcgagca cgcacgcgtg ccgtcgagcc agggaaattt ctgcagcgcc gccgccagtc
    22741 ctttcttcag gtcttcgaaa ggacggtcgc cccagtgctg gctgattgaa tcggtgaatg
    22801 cctggccata acccggtgac ccgtggaaat cgatgaatac cgtggcgtag ccgcgcgccg
    22861 cgaatgtctg ggcattccag cgccagttcc agtggttctg gaaactcacc tgcgggccgc
    22921 catgcacgat gaatgcaatg gggtatttct tgtcactggc gaagccgggc ggattcacga
    22981 gatacccgta cacggtttcg ttgttccagc ccgggaaact gaactgttcg aagctgccga
    23041 gcgtacggtt cgcgagcact tccgcgttcg cggtcgtgag ccgttgccgc tcggaattgc
    23101 ccgtctgtag atagagatcg ggcggcgagg cgagatcgtg ccacagcacc accgcgccgc
    23161 gcttcgccgg cgcgaaatcg cccacgttgc ccttgccgga gatcggcgtc gcacgtcccg
    23221 tggcgacgtc cactgaaaac aggggcgtct gcccgaagtc gctcgccgtc gcgagcagcg
    23281 tgcggccgtc gctcgccagc tccaggtgct gcaccgagcg gtcccagccc ggtgcaactt
    23341 cgcgcaccga ggcgccgctt cttaccttga tgcggaagcg gtcggcttcg aagcccggac
    23401 ggctcatggc gagccatgcc aggtcgccgt ttcgcaggta cacgggctgc gtgtcccacg
    23461 cgggattgtc gggagtcaga ttccgtgggg cggcgccgcc atccgaaggc acttcgtaga
    23521 gatcgaaatt cgtcgaccac gcctcttcgc gtcccgccac gcgcgcactg aacaccacgc
    23581 gcgagccgtc ggggctgaac ctgaactctt catcgccgcc gtcgggcctg gatggcacat
    23641 ccgcgttgag cgcgcggctc acgttcacgg gcggcccggc gcggccgtcg gcattaacag
    23701 gcgcgacgaa caggttcgag cgcaagccgg tgctccaggt atcccagtgg cgcacgaaca
    23761 ggccgtcgaa gacacgtccg gtttcctttt tcttgcccgc cgcgtcgagc cgctcccggg
    23821 tgcacttcgc gtcgacgcaa tccgggaaca cttccatgct gagagcgatg cgtcggccgt
    23881 ccggtgaaaa ccggaacgtg cccacttcga gcggatagtc ggtgatctgc acggcttcgc
    23941 cgccggtcag cggcaggcgc cagatctgct gtgagccggt acgcgacgaa agaaaataaa
    24001 cgctgctgcc atccacctgc cagcgcggat gtgcgtcgtt cgacgaatgt tgtgtcagct
    24061 gccgtggctt cggcttcgcg ttcgtgagat ccaggagcca caggtcggtg cggccgcggt
    24121 tggcttcgag atcggtcgtg cgctgcacgt acaccacgta acggccatcc ggtgacacct
    24181 gcggatcgct gacgcgggtc agcatgttga ggtcggtggg ggtcagggcg cgcgaggggg
    24241 catcggcagc tcccgcctgc gcgctccccg ccaacaccgc tgcaatgcac atccaggact
    24301 tcatgacgat ctccggtagt tagtcttttg ctagcagccg atttcggatg cttcgatgat
    24361 ccagctctcg gccgccgccg ccgccagcca ttcgagcatg tgctcatcgg cgagcacggt
    24421 ggccatgtac gccgccgtgt cgtcgcgcac cgtggcgcca taggtgcgca ggcgcaggac
    24481 cacgggcgcg tacatggcgt cggccacgct gaagtcgccg aacagccagg gcccgccggc
    24541 gccgaaccgg gcccggcaat cggcccacag gttctcgatg cgggcgattt ccgccgagcg
    24601 ttcgggattg ggcgcggtcc tgcggccggt ggctcgggcg ttcatgggcc attgtgaacg
    24661 caggatcgag aatcccgagt gcatttcggc gcacgcggcg cgcgctatgg cgcgtgcttc
    24721 gcgcgcccgg ggccagccgc ggcccgcgag ctcgcatgcg tactcgcaga ttgcgagcga
    24781 gtcccacagc agcagcgcgc catgcctcaa gacgggcacg cgccgtgtgg ggctggcggc
    24841 cgcgacgtct cgtgcgaatt ccggcgtatc gagcggcatg acgcgctcgg caaaatcgag
    24901 accgacgtgc ttcatgagca gccatggacg cagcgaccac gacgagtagt tcttgttgcc
    24961 catggtgagc aggatttcgt ccaagcggtt tcccccgtta acatgcacgc catgtccatt
    25021 ggcgcaatcc gcgcaatctg cttcgacctc gataacacgc tctgggaaat cgagccggta
    25081 ctgaatcgcg cggagcgcat tctcgccgat tggctcgagc gccgataccc acggattcgc
    25141 gaacgcttca ctgccgccga agtgctcgag gtgcgcgccg cgctgctcgt cgaacaaccg
    25201 caccaggcgc acgacttgtc gttcctgcgg cgtgaaacca tcgcgcgttg cgcgaccgag
    25261 gtgggttaca agcgggacat ggcccacgaa gcgttctcgg tctggcacgc cgcgcgcaac
    25321 caggtcgagc catatgccga ggtcattccg gcactcgaaa aactcaaggc gcgtttccgg
    25381 ttagcgagtt taagcaacgg caacgcggac ctgaaagtga tcggactcgc gcaccatttt
    25441 gaagtcacct tgagcgctgg cgcactggga tacgccaagc cggatccgcg cgcctacacc
    25501 gcgctcgcgg aggcgctgac actcaaaccc gcggaaatac tgtttgtagg cgacgaaccg
    25561 catgccgatg tggccggtcc acggacagct ggcatgcaaa ctgtatgggt gaatagaggt
    25621 ggtgttgttt ggcccgacgc gttgcccgcg gcggatgcca gcgtcaccga cctcgccggg
    25681 cttacggcgc tgctcacccg gaacctgtaa ccaggaactg tgaactgcgc accgtccgcc
    25741 cggacgtagt tagcagtgtg caccggttgc tgccagcggg tgcgcgcctg gtagcgtaaa
    25801 accggttcat gcgtcacgga accggcgctg ccaaagcgct caactggaag tcgccaggcg
    25861 ccgatgaatt gagtgcagtc attcgacggg gtgaacaatg gccatacagc cggttgcgag
    25921 ccttaaggaa ttcttccgcg acgcgttgca cgacgcgctg tcgcatcaac acgtggcggt
    25981 cgaaggtgag accgagcact acgtggtgaa tgtgctgacg ctgttctccg acgccgacgc
    26041 gctctacgag cgccagtcgt acaacgaggc gcgccacgcc gattcccgcc ggcagctgaa
    26101 accgctggcg atgatgttgg gcgaggcgct cgaagcgccc accagcgaag cccgtttccg
    26161 cggcctgcag cgcctcggtg acgtgtcgct gttcatcgcc ggattcttct ccgcgggttt
    26221 cgcccgcaag ctggtcgacg tggattacca catcgccatg ggcggacagg cctatggcgc
    26281 gctggccgag agttgtccgc ccgcgcgtgc ccggaccctg cgccaggtat tcgcggagct
    26341 cgccgcaaaa ttccagccca tggtcgacgc gctcaacgag atcagcgaaa ccgcctatca
    26401 acattcggac caggatcgcc tgcgcttgta cgagctgtgg gtgaagaccg gaagcgagcg
    26461 ctcccgccag ctgctgcgca agcttggcct cgagcccgca cccgctgcgc gaagtctgct
    26521 gtcgcactga catgaacgac gccactctct cttcgctgca gggtgcgcta actgaaatct
    26581 acgatctgcc ggcgacaccg gacgtgcgtg attttctgct gaccgatcgt gcccggctct
    26641 ccgccgtggg cggcccgcga gcgagcgacg aacagctcat cgtcgcggaa gagggcgata
    26701 ccctttcgtt ggcgctgtac atcgatgcgg cggtgctcga aaggctcgag cgttgcgatc
    26761 cgtttgccgg gctcacgcag cacaacctcg ccgactatct caccgtggcc gagggggtca
    26821 gtcatttcgt ctatgtcgcc tggaacaccg gctacgacaa gccggtcacc ctgctcgagc
    26881 tcgagctgca ggcggaggtc gacaagtacg tgctctgcgc ctggctgctg cgcaaacagg
    26941 gcgccggccg ctttccgcgc gagctgcatc gaatcctgtt tgaacgggcg cacgtggacc
    27001 ccgtggccgc cgccgggcgc attggcctgt accacacggc gtctgactac gcggcgcgct
    27061 tctgccggcg cgtggccgcg ctgctcgagc gcaagcgaaa tggcgttgcg cgcgacctca
    27121 tggccgagtt aaggcgtttc tatcgatggg ggaatgtgcg caagatgcat cacatcgagc
    27181 gctacgcctg aactcgtgtg aaaaggcgcc tgcggcgcgc tattcgacgt cgtggtcgtc
    27241 ctgggacttt cgctcgtcaa gctcgcgttt gagcttgatc cactcgccga gcttgcgcag
    27301 gtcgcgtttg accggcggct tcctggactc gggaatgtgt gctgcgccga cgcgcgcata
    27361 cggatcgaat ccgcccgcac gtttgcgctc caccgtgagc ttcgcgttgt cgccgcgggg
    27421 cggggtcgac tcgatggaca acggtgcgcg ttcgagcggt acgcctgcgt aaccggcgtc
    27481 gatccattcg acgcgcgcgt taccacgatc gtcgtgaacg atatgagaca cacggcgccg
    27541 gttggccgcc tgcgggggtt tgctgttcga attgttatct gacacagccc aagtatgact
    27601 gacctgacgt ttccccggtt tgacatagcg accatttcgg tcacctcgaa tcacggcctg
    27661 tgaggcgctt cacgcaattt ttcggcgtgc cgcggctcgc agaattccaa tgatggtctt
    27721 ggcgtcgttg agctcgccgc tgcgtgcttt ggcgacggcg tcttcgagcg gccaccattc
    27781 gacctcgaac acctcgccgt cctccggcgc cgattccacc ggtgtcaggt ccgtggccag
    27841 gtagagatgg atcacttccg tgaatacgcc gggcgagctg aaataggcgc cgagcgattc
    27901 ccactgccgg gccgatatcg cggcttcctc cgcgagctcc cgctgcacgg tgacctcggg
    27961 cggttcgttg ggttcgagct tccccgccgg cagctcgtag atgtagccgc cggccgcgtg
    28021 gcgaaactgg cgcagcaggc atacacggtt gccggcatcg atggccacgg ctgccgcgcc
    28081 acccggatga cggacgatct cgaggggcag gcgcacgcca ttgggcaggt ccacggtgtc
    28141 gacgctcacc ttgatgacgc gtccttcgaa aatttccttg cgatcgaaca gcatggcacg
    28201 agtgtactcg ccgtcacgta cggcgggtcc cacagtagta aggcgatggc ggaagagttg
    28261 tttgtcgcgc tcgatgcgtc cgcacaacag gcatggtgat gcttgtaccg gccggcggcc
    28321 cgcgctcata atcgccgcac cccgtgtccg gtcccgctcg cggggcaaat cgagatcaaa
    28381 aaggcgtcac attccggcca gataccggcg tgacggccgt cacggcttcc ggggagccgg
    28441 tcctaagatc aaccgccatt catatattgt tgtattgatg tccacggccg aacaactcgg
    28501 aagtctgaac gctccgcaac gcaaggcggt gacttacggt gagcctcttc ccggcgggaa
    28561 gggctttcgc gcaggtccgc tgctgatcgt cgccggtgcg ggtactggca agaccaacac
    28621 gctcgcgcat cgcgtggcac accttgcaat gagtggcgtc gaccccgcgc gcatcctcat
    28681 gctgacgttc acgcggcgcg cctccatcga gatgaagcgc cgcgccaacg aaatcatgcg
    28741 cgaagccttc gacgagaagc tcggcggcgg caaagcgcag gcgatttcgc aacggctcac
    28801 ctgggccggc acgttccact ccatcggcaa ccggctgttg cgccactacg ccaaacacgt
    28861 gggtctcgac ccggctttca gcgtgctcga tcgctccgat tcggccgacc tga
//
LOCUS       DASQJS010000019         5269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3153120, whole genome shotgun sequence.
ACCESSION   DASQJS010000019 DASQJS010000000
VERSION     DASQJS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3153120"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VEW08_00880"
     CDS             complement(<1..666)
                     /locus_tag="VEW08_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39306.1"
                     /translation="MRVFVSLWFLVALGIALPALAANSWVIHAAKIYPTPYAAPIKDG
                     IVVVRDGRITYAGKNDPAKIPKSTRTSDCNGGVLTAGFQNSHVHFIEPRWADAAHKPA
                     AELAQSLEDMLTRYGFTTVFDTTSDQANTFALRARVEKGEIRGPRIFTVGLGLFPPDG
                     IPSYINDLPPQVLARLHQPRTADEARAAVRANIAAGADGTKLFLHTSPRQGVITKMSA
                     DVAR"
     gene            complement(736..1767)
                     /gene="recA"
                     /locus_tag="VEW08_00885"
     CDS             complement(736..1767)
                     /gene="recA"
                     /locus_tag="VEW08_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011059579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HYJ39307.1"
                     /translation="MDENRKKALGAALGQIEKQFGKGSVMRLGDAAASYDVEAVSTGS
                     LGLDIALGIGGLPRGRVIEIYGPESSGKTTLTLQVVAEIQKLGGTAAFVDAEHALDPS
                     YAEKLGVNVNELLVSQPDTGEQALEITDMLVRSNAVDLVVIDSVAALTPKAEIEGEMG
                     EPQMGLHARLMSQALRKLTGNISRSNTMVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRLDIRRIGAIKNGEEVVGNQTRVKVVKNKVAPPFREAEFEIMYGMGISREGEIID
                     MGSAQGIVEKSGSWYSYNGERIGQGKDNARDFLKARPEIAKEIENQIRAKLIPVKAPR
                     PELKSVGSD"
     gene            complement(1869..2405)
                     /gene="thpR"
                     /locus_tag="VEW08_00890"
     CDS             complement(1869..2405)
                     /gene="thpR"
                     /locus_tag="VEW08_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HYJ39308.1"
                     /translation="MLRLFFALQPTLEQNTALTAHVAPLVAQLEARTVPAQNLHATLC
                     FIGAIEEERVDALRAAAASIRGRPVRLRFDALEYWETPKILCATSSRDSSAASELAIA
                     LGEAAVAAGFAPDIKPFRAHLTLARKIAAARVATVPLPLPLVPPMVMSADKFVLMSSR
                     REGEVSIYSAVDCWPLYD"
     gene            complement(2408..2920)
                     /locus_tag="VEW08_00895"
     CDS             complement(2408..2920)
                     /locus_tag="VEW08_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HYJ39309.1"
                     /translation="MNVPNDAALAALTREAGAQLHRAARRLVTAESCTGGWIAKLATD
                     IEGSSEWFDCGFVCYSNAAKTRDLGVPEKLIESDGAVSDAVVRAMAEGAIARTGANVA
                     LAVSGIAGPGGAVPGKPVGTVWFALAYKQEDRLECQGHLIQFAGNRDAVRRQAVEYAL
                     KLLRALPNHV"
     gene            3027..3545
                     /locus_tag="VEW08_00900"
     CDS             3027..3545
                     /locus_tag="VEW08_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HYJ39310.1"
                     /translation="MNTLYDPKRMTTYGIIAIILGILAMAAPVVTGLSIALLVGILVM
                     VGGVVRLVWAFQAGSVGEGILKFVLAGLTLVCGLLMVANPLFASGLLTILLSLYFIVD
                     GVAEIAVGLGTKRGWFIFAGAVSILLGVLLWAQFPLSGAWALGILLGIKLLFIGLVMI
                     MGGSAARSMATG"
     gene            3593..>5269
                     /gene="mutS"
                     /locus_tag="VEW08_00905"
     CDS             3593..>5269
                     /gene="mutS"
                     /locus_tag="VEW08_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HYJ39311.1"
                     /translation="MANDKLTGGAAGAASGHTPMMTQYLRIKAEHPGSLLFYRMGDFY
                     ELFYDDARRAARLIDITLTSRGQSAGEPIPMAGVPYHAVETYLSRLVRKGESVAICEQ
                     IGDPAKSKGPVDRQVVRVVTPGTVTDDALLEQRRETLLAAVSRRAGKDGVVFGLAWLD
                     LAAGRFSVLEADGDAALEAELERLKPAELLVPEDHAREIGVITHGEQRVRPPWHFEFS
                     SASRLLTDQLGTLDLRGFGADDLPLAISAAGALLQYVRETQKTALPHITTLAVEERRD
                     TLHLDASTRRNLEIDVSLSGQDSATLFALLDSTVTPMGSRNLRRWLNRPLTDQQELRR
                     RYQAIALLVDARRFEALREPLRAIGDVERILARVALRSARPRDLTSLRASIAALPALR
                     ASLKRIEAPLVVELTAAVGEHADVLTLLQQAIAEEPSVLLRDGDVIATGYDADLDELR
                     RIATHTDEFLLELERRERDRSGLSSLKLAYNRVSGFYIEVSRSQADNVPKDYIRRQTV
                     KNAERFITPELKGFEDKVLGAREKSLAREREIYETVLTQLTDRLAALQVTA"
BASE COUNT          915 a         1702 c         1656 g          996 t
ORIGIN      
        1 acgcgcgacg tccgctgaca tcttcgtgat gacaccctga cgcggtgatg tgtgcaggaa
       61 cagcttggtg ccgtcggcgc cggcggcgat gtttgcacgg accgcggccc tggcctcatc
      121 ggcggtgcgc ggctgatgca gacgagccag cacctgcggc gggagatcgt tgatgtagct
      181 gggaatgccg tcgggtggaa acagtccgag acccaccgtg aaaatgcgcg ggccccgaat
      241 ctcgcccttc tccacacggg cgcgcaaagc gaaggtgttg gcctggtccg aggtggtgtc
      301 gaacaccgtg gtgaagccgt accgcgtcaa catgtcctcg agagattgcg ccagttccgc
      361 ggcaggcttg tgagccgcgt cagcccaacg cggctcgatg aaatgaacat gactgttctg
      421 gaaaccggca gtcagcacgc cgccgttgca atccgacgtt cgtgtcgact tcggaatttt
      481 ggccggatcg tttttgcccg cgtaggtgat gcggccgtca cgcacgacga cgatgccgtc
      541 tttgatgggc gcagcgtatg gagtgggata gattttcgcg gcgtggatga cccagctatt
      601 tgcggccagc gctggcagag ctatgcccag cgcgacgagg aaccacaggg acacgaagac
      661 gcgcatgtgc tcaagagggg acagatctat ttttgccgaa gacggcaaaa aaagatcagt
      721 ccccggggtc ccccatcagt cagaaccgac gctcttgagt tctggacgcg gtgccttcac
      781 cgggatcagc ttggcgcgga tctggttttc aatttccttg gcaatctccg ggcgtgcctt
      841 caggaaatcg cgagcgttgt ctttgccctg gccgatgcgc tcgccgttgt agctgtacca
      901 ggaaccggac ttctcgacga tgccctgggc agagcccatg tcgatgatct cgccttcgcg
      961 cgagatgccc atgccgtaca tgatttcgaa ctcggcctcg cggaacggcg gcgcgacctt
     1021 gttcttgacc actttgacgc gagtctggtt gccgaccact tcttcaccat tcttgatggc
     1081 gccgatgcgg cgaatgtcga ggcgaaccga agcgtagaac ttgagcgcgt tgccgccggt
     1141 ggtggtttcc gggttgccga acatgacgcc gatcttcatg cggatctggt tgatgaagat
     1201 gaccatggtg ttcgagcgcg agatgttgcc cgtgagcttg cgcagcgcct gcgacatgag
     1261 gcgcgcgtgc aggcccattt gcggctcgcc catttcgcct tcgatttcgg ccttcggcgt
     1321 gagcgccgca accgagtcga tgacgacgag gtcgaccgcg ttggatcgca cgagcatgtc
     1381 ggtgatttcg agggcctgct cacccgtatc cggctgagag acgagcaact cgttcacgtt
     1441 gacgccgagc ttttcggcat acgaaggatc gagcgcgtgt tccgcgtcca cgaacgcggc
     1501 ggtgccgccg agcttctgga tttccgcgac gacctgcaga gtgagcgtgg tcttgccgga
     1561 agattccggc ccgtagattt caatgacacg gccacgcggc agaccgccga tgccgagcgc
     1621 gatgtcgagg cccagcgaac cggtggagac tgcttcgacg tcgtaagacg cggccgcatc
     1681 gccaagacgc atgaccgaac ccttgccgaa ttgtttctcg atctggccga gcgcggcgcc
     1741 aagcgccttc ttgcggtttt catccatcgc tgaatcccct tgtctttgcg tgcgagtttt
     1801 gatggaaaaa atttagcaca agctcctgta taaatacaca gctatttcta ttcgaaacac
     1861 tgagaatttc agtcatacag tggccaacaa tcgacagcac tgtatatcga gacctcgcct
     1921 tcgcggcgac tcgacatcaa cacgaatttg tcagcgctca tcaccatcgg tggcaccagc
     1981 ggcagcggca atggcaccgt cgcaacccgc gccgcggcaa tctttcgcgc cagcgtgaga
     2041 tgcgcgcgga acggtttgat gtccggcgca aatccggcgg caaccgcggc ttcacccagc
     2101 gcgatagcaa gctcactcgc cgccgacgaa tcccgggaag acgtcgcgca aagaatcttc
     2161 ggcgtctccc agtattcgag cgcgtcgaac cgcagccgca cgggccggcc gcgaatgctc
     2221 gctgccgctg cgcgcaatgc atcgacccgc tcctcttcta tggcgccgat gaaacacagc
     2281 gtggcatgca gattctgcgc gggtactgtc cgcgcctcga gctgcgcgac gagcggagcc
     2341 acatgcgccg taagggctgt gttctgttcg agcgtcggtt gaagcgcaaa gaacaaccga
     2401 agcatgttca gacgtgattt ggcagcgcgc gcaggagctt gagcgcgtat tcgaccgcct
     2461 ggcggcgcac ggcatcgcga ttgccggcga actggatgag atgcccctgg cactcgagac
     2521 ggtcttcctg cttgtacgcc agcgcgaacc acacggtacc caccggtttt cctggcacgg
     2581 cgccgccggg gccggcgatg ccgctgaccg ccagcgccac attggcgcca gtgcgcgcaa
     2641 tggcgccttc ggccatggcg cgcacgacgg cgtcgctgac cgcgccatcg ctctcgatga
     2701 gtttctccgg caccccgaga tcccgcgtct tggccgcgtt cgaataacag acgaatccgc
     2761 agtcaaacca ctcggagctg ccttcgatgt cggtggcgag tttggcgatc cagccgccgg
     2821 tgcaggattc ggcggtgacg agccggcggg ccgcgcggtg cagctgcgcc ccggcttcgc
     2881 gggtcagtgc cgcgagcgcg gcgtcgttgg gtacgttcac gccgcgattc tcgcatcgtt
     2941 ccaatccgtg cggttccttt gcagcgcccc atttcggcca gtgctaagaa ggcgcgggct
     3001 caatctcccc aaacaaggaa aaaccgatga atactcttta cgaccccaag cgcatgacta
     3061 cgtacgggat catcgccatc atccttggga ttctcgcgat ggcggccccg gtggttaccg
     3121 ggctctcgat cgcgctgctc gtcggaatcc tcgtcatggt cggaggcgtg gtccggctcg
     3181 tctgggcctt ccaggccggc agcgtcggcg agggcatcct gaaattcgta ttggccgggt
     3241 tgaccctggt ctgcggtctg ctcatggtgg cgaatccgct gttcgcttcc ggcttactga
     3301 ccatcctgct ttcgctgtat ttcatcgtgg acggcgtagc cgagattgcg gtcggcctcg
     3361 gaaccaagcg cggatggttc atcttcgcgg gcgccgtttc gattctgctc ggggtcctgc
     3421 tctgggcgca gttcccgctc tccggcgcct gggcgctcgg aatcctgctg ggcatcaagc
     3481 tgctgttcat cggcctggtc atgatcatgg gtggatcggc cgcacggtcg atggccaccg
     3541 gctagtacaa ttctcccgct gcgtcctcgt tgacggacgc agcgggagaa cgatggcaaa
     3601 cgacaaattg acgggcggtg cggcgggagc cgcttcgggc cacaccccca tgatgacgca
     3661 gtacctgcgc atcaaggccg agcatccggg ctctctcctc ttctatcgca tgggcgattt
     3721 ctacgagctg ttctatgacg acgcccgacg cgccgccagg ctcatcgaca tcacgctcac
     3781 ctctcgcggc cagtccgcgg gcgaacccat tccgatggcc ggtgtgccgt atcacgcggt
     3841 cgagacctat ctctcgcgtc tggtgcgcaa gggtgaatcg gtcgccatct gcgagcagat
     3901 tggcgacccg gcgaaatcca agggtccagt cgatcgccaa gtcgtacgcg tggtcactcc
     3961 gggcaccgtc accgacgacg cgctgctcga gcagcgccgc gaaaccctgc tggccgcggt
     4021 gtcgcggcgc gctggaaaag acggcgtggt ttttggactt gcctggctcg acctggccgc
     4081 cggccgcttt tcggtgctcg aggcggacgg cgacgccgcg ctcgaagccg aactcgagcg
     4141 gttgaagccc gcggagttgc tggttccgga agatcatgcg cgcgagatcg gggtgatcac
     4201 ccacggtgag caacgcgtgc gcccgccgtg gcacttcgaa ttctcgagcg cctcacggct
     4261 gctcaccgat caactgggca cgctcgacct gcgtggtttc ggcgccgacg acctgccgct
     4321 cgccatctcg gccgcgggtg cgctgctgca atacgttcgc gagacacaga aaaccgctct
     4381 gccgcacatc acgacgctcg ccgtcgaaga acggcgcgac acgctgcacc tcgatgcctc
     4441 cacacgccgc aatctcgaga tcgacgtgtc gttgtcgggc caggactcgg caacgttgtt
     4501 tgcgctgctg gattccaccg tcacccccat gggctcgcgc aacttgcgcc gctggctcaa
     4561 tcgtccgctc accgaccagc aggagctgcg ccggcgttac caggcgatcg ccttgttggt
     4621 cgacgcgcgg cgcttcgaag ccttgcgtga accgctgcgc gccatcggcg acgtggagcg
     4681 catcctcgcg cgcgtggccc tgcgcagcgc gcggccgcgc gacctcacca gcctgcgcgc
     4741 gtccatcgcc gcgctgccgg cgctgcgcgc cagcctcaag cgcatcgagg cgccgctggt
     4801 cgtcgaactc acggccgccg tgggcgaaca cgccgacgtc ctgacactgc tgcagcaggc
     4861 catcgccgaa gaaccgtcgg tgttgctgcg cgacggcgac gtgatcgcga ccggctacga
     4921 cgcggacctc gacgagctgc ggcgcatcgc cacgcacacg gacgaattcc tgctcgagct
     4981 cgaacgccgt gaacgcgacc gctccggcct ctcgtcgttg aagctcgcct acaaccgcgt
     5041 ctcgggcttc tatatagaag tgtcgcgttc gcaggcggac aacgtcccca aggactacat
     5101 ccgccgccag accgtgaaga acgccgaacg attcatcaca cccgagctca agggcttcga
     5161 agacaaggtg ctgggcgcgc gcgagaagtc actggcgcgc gagcgtgaga tttacgagac
     5221 ggtgctgacg caactcaccg accgcctcgc cgcgctgcag gtcacggcc
//
LOCUS       DASQJS010000020        18964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1268106, whole genome shotgun sequence.
ACCESSION   DASQJS010000020 DASQJS010000000
VERSION     DASQJS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18964
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268106"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VEW08_00910"
     CDS             <1..194
                     /locus_tag="VEW08_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39312.1"
                     /translation="DVNEILIEAHEHEKEGLEAYYHLLKLVEGKSILLEEYARDQIAA
                     EEMHLADIRKMMRKPGSIK"
     gene            complement(199..972)
                     /locus_tag="VEW08_00915"
     CDS             complement(199..972)
                     /locus_tag="VEW08_00915"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HYJ39313.1"
                     /translation="MTEFALAYTWIVTKSQPHADSLVIQLRRQGFRALAVSCIEHQWR
                     DWPELRRIGADGAAVLFVTSRAAAARIDVPHGITVAAIAPTTSATLEARGIKVRLSAS
                     GGARELAQAVNDSTAIPDGAEVFYPTSDAALRQPEHQAAVATLSQRLRVHTQAVYSTV
                     APGNLAQELGALRTSESLAQAPLGFCFWSPSSVENFASARGFELMPGPVVLIGGSTMR
                     CWRETAPPAWRRAYQHDAETPLEWSLRFLERDAVAADGS"
     gene            1082..2116
                     /gene="hemE"
                     /locus_tag="VEW08_00920"
     CDS             1082..2116
                     /gene="hemE"
                     /locus_tag="VEW08_00920"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HYJ39314.1"
                     /translation="MHSRERFLAALRGDALDRPPVWLMRQAGRYLPGYRALRAEHSFW
                     DVCQNPELSTRAALEPLDRYQLDAAIVFSDILVVPLAMGLGVSFGPGEGPRIAKPLRS
                     GADYDAWQLDGVLSRIAYLPRAVAHLKETLKDRYGILGFAGAPFTLFAYCVEGGGSDD
                     FREARTLMHRDPALARRALATIADVVADLLLAQVDAGADAVQLFDSWGGLLAPDEYRD
                     FALPALQRITAKLNARKVPTLLFVRGGHHLLPLLGESGVTGFSLDWRTEWQVARDMYP
                     KHLLQGNIDPVLLLGSADVIRSRTRELLETMKRTTGGRSCILNLGHGILPGTPPETVA
                     AMVETAVEFN"
     gene            2124..3203
                     /locus_tag="VEW08_00925"
     CDS             2124..3203
                     /locus_tag="VEW08_00925"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39315.1"
                     /translation="MIPLALVGMSYREAPSAVRAALSVFDSDASGPSKQLLDAAEITG
                     VVRIESCARVEWVLASERPSWAAELFSAALIGATENPDHVRPRVRHGAAAVGGLLRVA
                     AGLDSVAQGEHAIGNQVLRAFERAHEAGTVCRSLHVAWRGVGDLLNRARRILPQGRTG
                     GVQALVMQRLTSVPRDAPIAVFGLGEIGQAMLRALADAGFTAVESFNRASMRAFEGAV
                     QRVDTLIVASGAPHAWLKLPPAAGIRQVFDLGSPLQIVAAPGWRVTALDELLDGNGAV
                     LPEAEFAALEMECAGVAEKICQTLLAPPPSDTLAAMTAMRTEFMQNKLPALLEGMPPQ
                     RARKVTSEVNAMLHQFISAARRDAQ"
     gene            3200..4159
                     /gene="hemC"
                     /locus_tag="VEW08_00930"
     CDS             3200..4159
                     /gene="hemC"
                     /locus_tag="VEW08_00930"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HYJ39316.1"
                     /translation="MNLRNIGSRGSDLALWQSRTVLASLRNAAPAADWETEITVIATR
                     GDLDQSPLLVGKMEKGFFTRELELALLDRRIDLVVHSLKDLPTAEPEGLSNRTILPRA
                     SAADWLLVRHEFYAERDDGLLPLAAGARVGASSLRRGALLGRFAPQAVSVPLRGNVPT
                     RLRKLGDGQTVDAIVLAAAGLTRLKLDLSAFAVVELPPEWWIPAPGQGALAAQCRAGD
                     QEIEDQIALLADADCIRATRWEREFLRVIEGGCSTPFGCFVAGDRAHLGIATERGWAA
                     HSIDLPKDLLEVSERDSFIRNAVAACQPHIINPRTISRALRAR"
     gene            4071..5399
                     /locus_tag="VEW08_00935"
     CDS             4071..5399
                     /locus_tag="VEW08_00935"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HYJ39317.1"
                     /translation="MTHSSATPSPPVSHISLTLARSAELYARAEALIPAGVSSPVRAF
                     RKVGGTPVFFRQAQGAHVTDEDGNRYLDFCMAWGPLILGHAHPRVVEAVQRAAADGLA
                     FGTAHRAEGELAERVLAAYPYAKLVRFVVSGTEAVATAVRIARASNGRRRILKFDGCY
                     HGHVDSLMVKAGSGVITQGLADSAGVPAQVAADTLVIPLGDIAALEQAFKEQGKDIAA
                     AIIEPLPANNGLLQQSREYLQRLRDLTRANGTVLIFDEVITGFRFGYHGYDKLLGIEP
                     DLTTLGKIVGGGLPVAAVLGRRELLAQLAPLGPVYQAGTMAGNPVCLAAGIATLAELA
                     EGGAYRHIEMLGKHLDAAFARHALRNQAKLPRVGPIVWPYFDASAALPVAASSITSTA
                     VNEYHRRYRGWLMHGVYLPPSAYEVCFLSAAHTTADVDKLLDVLATSDPG"
     gene            5413..6444
                     /locus_tag="VEW08_00940"
     CDS             5413..6444
                     /locus_tag="VEW08_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYJ39318.1"
                     /translation="MRSPIRLWLAERAELIALGVTLLATASLVFWWTILLRGEMRSNE
                     ALERALLAREQGLTAQQVAERQVAIAAHHDRRNVMLVGESTLLALMLSGSLVVLFLLA
                     QRRKQQREDMEKLLQFTSHEFKTPVAGVKGLLQSLALGSIPEAQRAELIKLGQIECDR
                     LEHLAETILAYQRAMAREPGAGAQRIDVAQFIGDLLAHRASTSIGGEVEVASIDAVHV
                     LGDRDGLRVILENLLDNANKYGGGRVSIAAEIRNGLWCLGISDHGRGFEPAAAEKLFD
                     LHNRGSGEGVTHGAGLGLAIARQLARRMGGNVIAHSAGPGQGAVFTVHLPLAPHSELS
                     FPAEGVRHG"
     gene            6437..7129
                     /locus_tag="VEW08_00945"
     CDS             6437..7129
                     /locus_tag="VEW08_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ39319.1"
                     /translation="MAERIAIVEDERLVRELVAVNLRHAGYEIVTAENFADGRQLLAG
                     GEFQLAILDVMLPGGDGFALTRYARDLGVNAPILMLTARSDTTSKVRGLDAGADDYLP
                     KPFDVNELLARVRALLRRASGRPATAPPRLAFASYWVRFDTGQALTNEGEVSLSDKEL
                     RLMEVFSANENRALTRTDLLEEVWGMDQFPTDRTVDNFILRLRKLFEPDPENPVHIVT
                     LRGRGYLFRSAP"
     gene            7178..8140
                     /gene="hemF"
                     /locus_tag="VEW08_00950"
     CDS             7178..8140
                     /gene="hemF"
                     /locus_tag="VEW08_00950"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HYJ39320.1"
                     /translation="MSTAQPLRVTPIGVTEIDAAREYFVGLHTRLTEAWKLLDGASDQ
                     RRDEWQRPAGDVLSGSGRLSLIENGATFDRAGVAFSDVSGARLPAAASERHTELAGQP
                     FRAMGTSCVVHPVNPYAPTSHANVRLFTARGGEVWWFGGGFDLTPVYAFADDARSWHE
                     AARAACVAAGPRAYALLKDACDKYFYLPHRGETRGIGGLFADDLNDTTAEIGGDFEHC
                     FALIRRIGDAYLDTYTKIVRLRAATPFGTREQDFQRLRRGRYVEFNLAFDRGTRFGLQ
                     SQARTESLLMSMPPRAEWRYDYRPVPGSPEAALADYLKPRDWLS"
     gene            8151..9161
                     /gene="hemH"
                     /locus_tag="VEW08_00955"
     CDS             8151..9161
                     /gene="hemH"
                     /locus_tag="VEW08_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HYJ39321.1"
                     /translation="MKWGVLLVNSGTPASLATRDVRAFLAGLLGDPRVVELPRVLWWP
                     ILHGIILRIRPRASARKYAAIWTPEGSPLAIESEALRRGLEASLRSHDTPVALAMLYT
                     GGASVPDAIASLRKAGAGGILTIPMFPQYCGASTGAVFDQVSAALRGLRSVPSLRFVS
                     SYHDDAAYIDALAASVEDHRRAFGATNHLLMSFHGVPERYVSLGDPYRDQCERTASLV
                     AQRLGLSAGEWSVSFQSRFGRARWLQPYTSEVLAAMPARGVKTVSVVCPGFAADCLET
                     LEEIGMENRDVFLAAGGEKFHYIAALNARADHVAALSALVSRELNALHPSGVKESTAC
                     FG"
     gene            9149..9580
                     /gene="hemJ"
                     /locus_tag="VEW08_00960"
     CDS             9149..9580
                     /gene="hemJ"
                     /locus_tag="VEW08_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HYJ39322.1"
                     /translation="MLWLKAFHIIGVVSWFAALLYLPRLFVYHSQIQGASIDDDAGNA
                     RFKVMERKLFVIMTIGAVAAAGFGVAMLALAPDYLLMRWLHVKLALVLVLVGYHGACF
                     ALLQDFAADRNSHSERWYRLFNELPAVLLIAIVILVVVKPF"
     gene            9688..9876
                     /locus_tag="VEW08_00965"
     CDS             9688..9876
                     /locus_tag="VEW08_00965"
                     /inference="COORDINATES: protein motif:HMM:NF022103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HYJ39323.1"
                     /translation="MPPTHIKKAPPAASPPSAAPPAPERTVSTKELLGGGHRLWIEHA
                     AERYLLQVTRSGKLILTK"
     gene            9995..10249
                     /locus_tag="VEW08_00970"
     CDS             9995..10249
                     /locus_tag="VEW08_00970"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ39324.1"
                     /translation="MTFPSQFESNDPDAWHALGIALAGIGDRVGAFTALRMAALLDGT
                     RASTQLALGNLFFDTGRLDDALRCFESAAAHDPTRSHGET"
     gene            10337..12082
                     /locus_tag="VEW08_00975"
     CDS             10337..12082
                     /locus_tag="VEW08_00975"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HYJ39325.1"
                     /translation="MRASESLTATSLIVAALLTGCSREPAEKPAEATAPADAVQVAAE
                     WPAFVESFIEARFNADPHFAVQAGRHEFDGKMPDLSRAAIDREVSDLRRFAAELGKRN
                     PASLTAAQRFEHEFLQWVVDTQLFWLTEADAPFRNPAWYMEKLDPSMYLTREYAPLPK
                     RLEGFLGYARAVPALAANIRANLRTPLPKAFIDRGTAGFGGYATFFRSEMPAIFAQVA
                     DEELKRDLTEATAAAATSMDELTAWLESQRATATDDFALGAPLFLAMLRQTERVDIPL
                     EELELVGRKDLERNLAALKDACAAFAPKASLAACVDRMRANKPSGGSVEGARAQLEDL
                     RQFVIDKKLVSIPGREQALVAESPPYNRGNFAYISIPGPYESPAVKVTYYVAPPDAKW
                     SAAERTAYLPGKAYLLFVSAHEVWPGHYLQSQFMNANPSRAEALWWNYSFGEGWAHYA
                     EEMMYDAGLGAGQPEMRVGMLINALMRNVRFLSAICLHSGCMSLEESEKMFRDSAFTD
                     AGNARQQALRGTYDPGYLAYTLGKLMIRKLRADWLAANPSATPQQFHDKFLSFGAPPV
                     PLVRKEMLGSTGAVL"
     gene            12264..12698
                     /locus_tag="VEW08_00980"
     CDS             12264..12698
                     /locus_tag="VEW08_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39326.1"
                     /translation="MGKNKMVFSAAACAAMVGVALAQVQASSTVYLIDVDPARYAGPA
                     GQRVMLRLGDQPRNVTIGTQDEICVQLQRVEGTEVISARVRALRPEPKTAAEIPLVGP
                     NIAPGTYRSTPWGLLLRAMPGTTDAQAFVKAFGTDNSPVCTL"
     gene            complement(12707..13579)
                     /locus_tag="VEW08_00985"
     CDS             complement(12707..13579)
                     /locus_tag="VEW08_00985"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ39327.1"
                     /translation="MRVAFLGLGKMGAGMAARMLAAGHSLAVYNRTAERAAPFAKLGA
                     RIAESPSAAAQKADVIIGMTADDDSSRAMWLGNQGALAADNVPHALAIECSTLSHDWV
                     LELNDRVRERRFRFVDAPVTGLPDAAATGTLTLLVGADAADLDAARPIFASLATRVLH
                     FGAVGQGTVYKLMINLLGAVQIASAAEAVVLAGRAGLDLKLVADAVANGQAASPQVVR
                     NVRRFVAGDHGTNVHFTPALRLKDIEYALRLAKKLGVDCPFGSVAAELYRKVCAQGGG
                     RDNESRIIDSLLKD"
     gene            complement(13579..14649)
                     /locus_tag="VEW08_00990"
     CDS             complement(13579..14649)
                     /locus_tag="VEW08_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39328.1"
                     /translation="MIQLFRTLVACATLVGGAHAQAAPGHVDITWMSIANMHFDLGSQ
                     KIVADGYITRLPPEIFYSGASGYGKTRLAARPDEAAVKEVLDALGGKPAVNLLLTGHN
                     HFDHSFDTGTWAKLSGARVIGAPTACLQTRAQGIAASRCTPVIGGEVFELERGVHVYV
                     IRWNHSGGPAQNPELHEPRELEGLPVPDARGALRAGVLEDFPNGGGNRAYLFMVDGPQ
                     GRFSWFFQDSGSPDDLREPVVLAGKDYGAPLDNLIAAMKAAGIDAVDLWIATGGRELA
                     ALVLPVLRPKAYLPVHWDGLFGPFKAGPLQPYDDPELAALLTEQGVRRIVPVQYMDKW
                     RLDPRGIRAVDNSVVKRKLGFN"
     gene            complement(14682..15449)
                     /gene="kduD"
                     /locus_tag="VEW08_00995"
     CDS             complement(14682..15449)
                     /gene="kduD"
                     /locus_tag="VEW08_00995"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HYJ39329.1"
                     /translation="MANSFDRSFDLGGRVAAVTGANTGLGRAIAEALAAAGADVACIG
                     RSDPADTLTAVRSFGRRGHWVNADLGAKPDYQRIVADIVGALGGLHILVNNAGIIRRN
                     NAIDFTEADWDAVLDVNLKSVFFLSQAAARHMIPAGGGKIINIASMLSFQGGIRVPSY
                     TASKSGIAGLTKLLANEWAVSGINVNAIAPGYFATNNTAALQADVKRNAEILGRIPSG
                     RWGDPKDLGGAAVFLASSAANYVQGIILPVDGGWLAR"
     gene            complement(15452..16288)
                     /gene="kduI"
                     /locus_tag="VEW08_01000"
     CDS             complement(15452..16288)
                     /gene="kduI"
                     /locus_tag="VEW08_01000"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005886840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HYJ39330.1"
                     /translation="MKFDTRHASHPSIIATADTAKLRELYHIPAMFTPDDVNLTFSHI
                     ERMINGGVMPVSKTIELNEVPALNKGPFLARRELGIINVGGPGKVIVDGQTTALAARE
                     GLYVPMGTTALSFSSDSKDAPAKFYLASTPAHARYDLVKIDIAKANPMPVGAAATANE
                     RTIYQYINPDICKSAQLLMGLTSLKEGSVWNTMPCHLHERRAETYFYFDLKPDARVVH
                     IMGEPHETRHIFVANEEAVISPPWGIHTGCGTTNYSFIWAMGGENQEYKDVAWVPMNV
                     LR"
     gene            complement(16404..17504)
                     /locus_tag="VEW08_01005"
     CDS             complement(16404..17504)
                     /locus_tag="VEW08_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYJ39331.1"
                     /translation="MTRIRKKAAGNRGMPKLAVPAKVTGIKPGRNATINDIARLASVS
                     KKTVSRVINQSPLVQEATRARIQAVIDKFGYVPDPQARGLAFRKAFLIGLVYDNPNAQ
                     YIVNCMEGALDAVRNSEYEVVVHPCDRKKPDFITGVRRFVERQKLRGVILLPPVSEND
                     ELTRALTAADCSYVRITYTQLDDPSRMVISNDRLAVAEVANYLVSLGHKRIGYIAGPP
                     GFRSAVERLAGFREALVARGVPFPSDLIVEGGYTYESGVAGGEKLLALSPRPTAIFAS
                     NDEMAAGVYRVANRLGLSIPGDLSIVGFDDGPLASRLLPSLTTIRLPIRELGRLAANK
                     ILHPETAGGTHAMVTPLEPHLVIRDSCQPPPR"
     gene            complement(17558..18388)
                     /locus_tag="VEW08_01010"
     CDS             complement(17558..18388)
                     /locus_tag="VEW08_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYJ39332.1"
                     /translation="MVSVVSDLAFDAKGVAAEFVKARRSAGSFTQYPGTQPQDLDSAY
                     RCQDEAISLWDDSIAGWKVGWIPEPLSHKYGAQRLVGPIFSGSVKHANGSASIEVPVF
                     ANGFAAIEAEFVVQLAKDAPANVTEWTTETARRFVKAMYIGIEIASSPLVNINDYGPA
                     VVASDFGNNAGLLLGAEVTDWQTRSLESLNCETRIDTNVVGRGGAAAVSGGPLAALAF
                     ALRCNARRGRPLRAGDYISTGAATGVHSIGAGQSAEAIFTGVGSLKCVTVPMTPVTDR
                     "
     gene            complement(18578..>18964)
                     /locus_tag="VEW08_01015"
     CDS             complement(18578..>18964)
                     /locus_tag="VEW08_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HYJ39333.1"
                     /translation="AIVEAELRAAQGSPAFAYQLDWGSPQDGGKWGAFHTLDIPLMFG
                     TLTARESLTGDGEEARRVSSAMQQALLAFCRNGDPNYQGLPRWEPYALPRRATMVFNV
                     ESRLVDDPRGNERQLFAKVPFIQQGT"
BASE COUNT         3223 a         6036 c         6230 g         3475 t
ORIGIN      
        1 acgacgtcaa cgagatcctc atcgaagcgc acgaacacga gaaggagggc ctcgaagcgt
       61 attaccacct gctcaagctc gtcgagggca agagcatcct gctcgaggaa tacgcgcgtg
      121 accagatcgc ggccgaggaa atgcatctcg cggatatccg caagatgatg cgcaagccag
      181 ggtccatcaa ataactagct aactaccgtc ggcggctacg gcgtcgcgct cgagaaaacg
      241 cagcgaccac tcgagcggcg tttccgcgtc gtgttgataa gcgcgccgcc aggcgggcgg
      301 cgccgtttcc cgccagcagc gcatcgtcga gccgccgatc agcaccacgg gacccggcat
      361 cagttcgaaa ccccgcgcgc tcgcgaaatt ctcgacgctc gacggactcc agaagcagaa
      421 tccgagcggt gcctgagcca gggattcgga cgtccgcaag gcgccgagct cctgcgcgag
      481 attgccgggc gccacggttg aatagaccgc ctgcgtatgc acgcgcaggc gctgtgacag
      541 cgtggccacc gctgcctggt gctcgggctg gcgcagggcg gcatcactgg tgggatagaa
      601 cacttccgcg ccgtccggaa tggccgttga atcgttcacc gcctgggcca gctcgcgcgc
      661 gccgcctgac gcggagaggc gcaccttgat gccgcgcgcc tcgagcgtcg ccgaggtcgt
      721 cggtgcaatt gcagcaaccg tgatgccgtg gggtacgtcg atgcgcgctg ccgcagcccg
      781 gctggtgacg aacagcaccg cggcgccgtc ggcgccgatg cgtcgcaact cgggccagtc
      841 gcgccactga tgctcgatgc aggaaaccgc aagcgcgcga aagccttgcc gacgcagctg
      901 aatgaccagg ctgtcggcgt gcggctgcga cttcgtgacg atccaggtgt atgccaacgc
      961 gaactccgtc atgaatgtta gccctcatgc ccggccaacg cttcgagcgt gacacagctg
     1021 tgacagttga ggctcgtgtc cgccgtacgc tttcacttga tttaaatgga gtttatccac
     1081 gatgcattcg cgtgagcgat ttctggcggc gctgcgcggc gatgcgctcg accggcctcc
     1141 ggtgtggctg atgcgccagg ccgggcgcta ccttccgggc tatcgcgcac ttcgcgccga
     1201 acacagcttc tgggatgtct gccagaaccc ggagctgtcg acgcgcgccg cgctcgagcc
     1261 gctcgatcgc taccagctcg acgccgcaat cgtgttctcc gacattctcg tggtaccgct
     1321 cgccatgggt ctcggagtca gcttcgggcc cggcgaaggt ccgcgcatcg cgaaaccgct
     1381 gcgaagcgga gccgactatg acgcctggca gctcgacggc gtgctttcgc gcatcgccta
     1441 tctcccgcgc gccgtggcgc atctgaaaga aacgctcaag gatcgctacg gtatcctcgg
     1501 cttcgccgga gcaccgttca cgctcttcgc ctactgcgtc gagggcggtg gcagcgacga
     1561 ttttcgcgaa gcccgcactc tgatgcatcg ggatccggca ctcgcccggc gtgcgctcgc
     1621 caccattgcg gacgtcgtcg ccgatctgct gctggcgcag gtggacgcgg gcgccgatgc
     1681 ggtgcagttg ttcgattcct ggggcggcct gctcgcgccc gatgaatatc gcgacttcgc
     1741 cctgccggcc ctgcagcgca tcaccgcgaa gctcaatgca cgcaaggtgc ccacgctgct
     1801 gttcgtgcgc ggcgggcatc acctgttgcc attactcggc gaaagcggcg ttaccggctt
     1861 ctcgctcgat tggcgcaccg aatggcaggt ggcgcgcgac atgtacccca agcacctgct
     1921 gcaggggaac atcgaccccg tgttgttgtt aggcagtgcc gatgtcatcc gctcgcgcac
     1981 gcgtgaactg ctcgagacca tgaaaagaac cactggcggc cgcagttgca tcctcaatct
     2041 cggccacggc atcctgcccg gcacgccacc ggagacggtg gcagcgatgg tggaaaccgc
     2101 cgtggaattc aactgacgcc gccatgatcc cgctggctct cgtcggcatg agttatcgcg
     2161 aggcgccctc ggcggtgcgc gcggcgctct ccgtgttcga ttccgacgcg agcggaccgt
     2221 cgaagcaatt gctcgatgcg gcagaaatca ctggcgtcgt gcgcattgaa agctgcgcac
     2281 gagtcgagtg ggtgctggcg tccgagcggc cgtcctgggc cgcggaattg ttcagcgccg
     2341 cgctcatcgg cgccacggaa aatcctgatc atgttcggcc gcgcgtgcgg cacggtgccg
     2401 cggcggtagg tggacttctg cgcgtcgccg ccgggctcga ttccgtggcg cagggcgagc
     2461 acgccatcgg gaatcaggtg ctgcgcgcct tcgagcgcgc gcacgaggcc ggcacggtgt
     2521 gtcggagctt gcatgtcgcg tggcgcggcg tcggcgacct gctgaaccgg gcgcgccgca
     2581 ttcttccgca gggccgcacg ggtggcgtgc aggcgctggt gatgcagcgg ctcacttccg
     2641 tgccgcgaga cgcgccaatc gcggtattcg ggctcggtga aatcggtcag gccatgctgc
     2701 gggccctggc ggacgcggga ttcaccgctg tcgagagctt caatcgcgcg agcatgcggg
     2761 catttgaagg tgccgtgcag cgcgtggata cgctgatcgt cgcgagcggc gcaccgcacg
     2821 cctggctcaa gttgccgccg gccgccggaa tcaggcaggt gttcgatctc ggcagcccgc
     2881 tgcagatcgt cgctgcaccg ggctggcggg tgaccgcgct cgacgaattg ttggacggca
     2941 atggcgcggt gttgccggaa gcggaattcg ccgcgctcga aatggaatgc gccggagtcg
     3001 cggagaagat ctgccagaca ttgctcgcgc cgccgccgag cgacacactc gcggcgatga
     3061 ccgccatgcg caccgaattc atgcaaaaca aactacccgc gttgctcgaa ggtatgccgc
     3121 cgcagcgcgc acgcaaggtg acctccgagg tcaatgccat gcttcaccag ttcatctcgg
     3181 ccgcgaggcg cgacgcgcaa tgaatcttcg caacatcggc agccgcggca gcgacctggc
     3241 gctgtggcaa tcccgcacgg ttctcgcatc gctgcgcaac gcggcgcccg cggccgactg
     3301 ggagacggag atcaccgtca tcgccacgcg tggcgatctc gatcagtcgc cgctgctggt
     3361 cggcaagatg gagaagggct tcttcacgcg cgagctcgag ctcgcgctgc tcgatcgccg
     3421 catcgacctc gtggtgcatt cgctcaagga cctgcccacg gccgaacccg aagggctttc
     3481 gaatcgcacg atattgccgc gcgccagcgc cgccgactgg ctgctggtgc ggcatgaatt
     3541 ctacgcggag cgcgatgatg gcttgctgcc gctcgcggcc ggcgctcgcg tcggcgcttc
     3601 gtcgctgcgt cgcggcgcct tgttaggccg cttcgctccg caggcggtct ccgtaccgtt
     3661 gcgcggcaac gtgccaacgc gtctgcgcaa gctcggcgat gggcaaaccg tggatgccat
     3721 cgtgctggcg gctgccgggc tcacacgcct gaagctcgat ctgtccgcgt tcgcggtagt
     3781 ggagttgcca cccgagtggt ggatccccgc ccccggccag ggagcgctgg cggcgcaatg
     3841 ccgtgccggc gaccaggaaa tcgaagacca gattgccctg ctggctgatg cggattgcat
     3901 ccgggcgacg cgctgggaac gggagttcct gcgcgtcatc gagggtggct gctcgacgcc
     3961 attcggctgc ttcgtggccg gcgatcgcgc gcatctggga attgccaccg agcgcggctg
     4021 ggcggcgcac agtatcgacc taccaaaaga cttgttagag gtttcagaac gtgactcatt
     4081 catccgcaac gccgtcgccg cctgtcagcc acatatcatt aaccctcgca cgatcagccg
     4141 agctctacgc gcgcgctgag gcgctgattc cggcgggcgt ttcgagcccg gtgcgcgctt
     4201 ttcgcaaggt gggcggcacg cctgtcttct tccgtcaggc gcagggtgcc catgtcaccg
     4261 acgaagacgg caatcgctat ctcgatttct gcatggcatg ggggccgctg atcctggggc
     4321 acgcgcatcc acgcgtggtc gaggcggtgc aacgcgccgc ggctgacggt ctcgcgttcg
     4381 gtaccgcgca tcgcgccgaa ggcgagctcg cggagcgcgt actcgcggcg tatccgtacg
     4441 cgaagctggt gcgcttcgtg gtcagcggga ccgaggcggt tgccaccgcc gtgcgaatcg
     4501 cgcgcgcgtc caacggtcgc cggcgaatcc tcaagttcga tggttgttac cacggacacg
     4561 tggattcgct catggtgaaa gcgggcagcg gcgtcatcac gcagggtctc gccgacagtg
     4621 ccggagtacc ggcgcaggtg gccgccgata cgctggtcat tccgctcggc gacatcgctg
     4681 cgctcgaaca ggcgttcaag gaacagggca aagacattgc cgcggccatc atcgaaccgc
     4741 tgccggcgaa caacggcctg ctgcagcagt cgcgcgaata cctgcagcgc ctgcgcgacc
     4801 tgactcgcgc gaatggcacc gtcctcatct tcgatgaggt aattaccggc ttccgcttcg
     4861 gttatcacgg ttacgacaag ttgctgggca tcgagccgga cctcactacg ctgggcaaga
     4921 tcgtcggcgg cggactgccg gtggccgcag tgctgggacg gcgcgagctc ctggcgcaac
     4981 tggcgccact ggggccggtg taccaggcgg gcaccatggc gggcaatccg gtgtgcctgg
     5041 ctgccggaat tgcgacgctg gcggaactgg ccgaaggcgg cgcctaccgg cacatcgaga
     5101 tgttaggaaa gcacctcgat gccgcgttcg cgcggcacgc gctccgcaac caggcaaagc
     5161 tgccccgggt ggggccgatt gtgtggccgt acttcgacgc gtcggcggca ctgcccgtgg
     5221 ctgcgtcgtc gatcacctcg accgcggtga acgagtatca ccggcgctat cgaggctggc
     5281 tgatgcatgg cgtctatctg ccgccgtcgg cctacgaagt gtgttttctg tcggcggctc
     5341 acaccacggc cgatgtggat aaactgctgg acgtactggc aacgagcgac ccaggttagt
     5401 ggaacacgcg tgatgcgatc tccgattcga ctctggcttg cggaacgcgc cgagctgatc
     5461 gcgcttggcg tgacgctgct cgcgactgcg tcgctggtgt tctggtggac catcctgttg
     5521 cgcggcgaga tgcgcagcaa cgaggcactc gagcgtgcac tgcttgcgcg ggagcagggg
     5581 ctgaccgcgc agcaggtcgc cgaacgccag gtcgcgatcg ccgcgcatca cgaccgccgc
     5641 aacgtcatgc tcgtcggcga gtccacgctg ctggcgctga tgctctctgg cagcctggtc
     5701 gtgttgttcc tgctggcgca acggcgcaaa cagcaacgcg aagacatgga aaaactgctc
     5761 cagttcacga gccacgaatt caagacgccg gtggctggtg tgaaggggct gttgcagagc
     5821 ctcgcgcttg gcagcattcc cgaggcgcag cgcgccgagc tgatcaagct cgggcagatc
     5881 gagtgcgatc ggctggaaca tctggctgag acaattcttg cgtatcagcg cgcaatggcg
     5941 cgcgagccgg gtgcgggcgc ccagcgtatc gatgtggcgc aattcatcgg cgatctcctc
     6001 gcgcatcgcg cgagcacctc gatcggcggc gaagtcgagg ttgcgtccat cgacgccgtg
     6061 cacgttctgg gagaccgcga cggcctgcgc gtgatcctcg aaaacctcct ggacaacgcc
     6121 aacaaatatg gtggcggccg cgtgagcatt gcggcagaaa ttcgcaacgg actctggtgc
     6181 ctcggtatca gtgatcacgg ccgcggcttc gagccggcag ccgcggagaa actgtttgac
     6241 ctgcacaatc ggggcagcgg tgaaggcgtc acgcatggcg ccgggctggg gctcgcgatc
     6301 gcacggcaac tcgcgcgtcg catgggcggc aacgtgatcg cgcacagcgc ggggccgggc
     6361 cagggcgctg tctttacggt tcatcttccg ctggcgccgc actcggagtt gtcgttcccg
     6421 gccgagggcg tgcgccatgg ctgagcggat cgccatcgtc gaagacgagc gtctggtgcg
     6481 tgagctcgtg gccgtgaacc tgcgtcatgc cggctacgaa atcgtcaccg cggagaattt
     6541 cgcggacggc cggcaattgc tcgcgggcgg cgaattccag ctcgccattc tcgacgtgat
     6601 gcttcctggc ggcgacggct ttgcgctgac gcgctacgcg cgcgatctcg gtgtcaacgc
     6661 gcccatcctc atgctcaccg cgcgcagcga cactacctcg aaggtgcgtg gcctcgatgc
     6721 cggcgccgac gactatctac ccaagccatt cgacgtgaac gaactgctgg cgcgcgtgcg
     6781 cgcgctgctg cgccgtgcga gcggccgtcc cgcgacggcg ccgccgcggc tggcgttcgc
     6841 cagctactgg gtgcgattcg acaccggcca ggcgttgacc aacgaaggcg aggtgtcgct
     6901 gtccgacaag gagctgcggc tcatggaagt gttctcggcg aacgaaaatc gggcactcac
     6961 gcgcactgac ctgctggaag aggtctgggg catggaccag ttcccgaccg atcgcaccgt
     7021 ggacaacttc atcctgcgcc tgcgcaagct gttcgagccc gatcccgaaa acccggtgca
     7081 tatcgtcacg ctgcgaggac gcgggtacct gttccggagc gcgccgtgag cgtcgcaccg
     7141 ggaggtgcgc aatgagcgtc gcaccgggag gtgctcagtg agcacagccc agccgctgcg
     7201 cgtcacgccc ataggcgtca ccgaaatcga cgctgctcgc gaatacttcg tcgggctgca
     7261 cacgcgactg accgaggcgt ggaaattgct cgacggggcc agcgaccaac gccgtgacga
     7321 atggcagcga cccgcgggcg atgtgttgag tggcagcggc cggctctcgc tcatcgagaa
     7381 tggagccacc ttcgatcgcg ccggcgtggc attcagcgac gtcagcggcg cgcgcctgcc
     7441 agcggcggca agcgagcggc acaccgagct cgcgggccag ccgtttcgcg ccatgggcac
     7501 gagctgcgtg gtgcatcccg tcaatccgta tgcgccgact tcgcatgcga acgtacggct
     7561 gttcacggcg cgtggcggtg aggtctggtg gtttggcggc ggcttcgatc tgacgccggt
     7621 ttatgcgttc gccgatgacg cacgctcgtg gcacgaggcc gcgcgcgccg cctgcgttgc
     7681 ggcggggccg cgtgcctacg cgttgctcaa ggacgcctgc gacaaatatt tctacctgcc
     7741 gcaccgcggt gaaacccgtg gcattggcgg gctgttcgcc gatgatctaa atgacaccac
     7801 ggcggaaatc ggcggggact tcgagcattg tttcgctctc atccgtcgca tcggcgatgc
     7861 gtacctggac acgtacacga aaatagttcg attgcgcgcg gccacgccgt ttggcacacg
     7921 agagcaggac ttccagcggc tgcgccgcgg acgctacgtg gaattcaacc tggcattcga
     7981 tcgcggcacg cgtttcgggt tgcagtccca ggcgcggacc gaatccctgc tcatgtcgat
     8041 gccgccccgc gcggagtggc ggtacgacta tcgcccggta cctggctcgc cggaagcggc
     8101 gctcgccgac tacctgaagc cgcgcgactg gctgtcatga gttccttgtc atgaaatggg
     8161 gcgtgctcct ggtcaattcc ggcacgccgg cgtctctcgc cacgcgcgac gtgcgggcat
     8221 tcctcgccgg attgttgggc gacccgcgcg tggtcgagtt gccgcgcgtg ctctggtggc
     8281 caattctcca tgggatcatc ctgcgaattc gtccgcgcgc cagcgcaagg aagtacgccg
     8341 caatctggac gccggaaggt tcgcccctcg ccatcgaaag tgaagccctg cgtcgtggtc
     8401 tcgaagcgtc cctgcgttcg cacgacacac cggtcgcgct cgccatgctt tacacgggtg
     8461 gcgccagcgt gcccgatgcc atcgcttcgc tgcgcaaggc cggcgccgga ggcattctca
     8521 ccattccaat gtttccgcaa tattgcggcg cgtcgaccgg cgccgtattc gatcaggtca
     8581 gcgccgcact tcgtggcttg cgcagcgttc cgtcgcttcg attcgtctcc agttatcacg
     8641 acgatgccgc ctacatcgat gcgctggcgg ccagcgtgga ggaccatcgg cgggcgttcg
     8701 gggccacgaa ccacctgctc atgtcgtttc acggcgtacc cgagcgctat gtctcgctcg
     8761 gtgaccctta ccgcgatcaa tgcgaacgaa cggccagcct cgtggcgcag aggctcgggc
     8821 tctccgccgg tgagtggagt gtgtccttcc agtcccgatt cggacgcgcg cgctggctgc
     8881 agccgtacac cagcgaagtg ctggcagcca tgccggcacg tggagtcaag accgtcagtg
     8941 tcgtctgccc aggcttcgcc gccgactgcc tcgagaccct cgaggaaatc gggatggaaa
     9001 accgcgacgt attcctggcg gcgggtggtg aaaaatttca ctacatcgcg gcgctcaatg
     9061 cgcgtgccga tcacgtggcc gcgctcagtg cactggtgtc acgcgagctg aacgcgctgc
     9121 atccttccgg cgtcaaggaa tccaccgcat gctttggctg aaagcatttc acatcatcgg
     9181 agtggtgagc tggttcgcgg cgctgctgta tctgccacgg ctgttcgttt atcactccca
     9241 gatacagggc gcgtcgatcg acgacgatgc gggcaacgcg cgtttcaagg tcatggagcg
     9301 caagctgttc gtgatcatga cgatcggcgc cgtggctgcg gccggcttcg gcgtggccat
     9361 gctcgcgctg gcgccggact atctactcat gcgctggctg cacgtgaagc tcgcgctggt
     9421 gctggtgctg gtcggttacc acggtgcttg ctttgcgctg ctgcaggatt tcgccgccga
     9481 ccgcaattcg cattcggagc gctggtaccg gctgttcaac gagctgcccg cggtgctgct
     9541 catcgccatc gtcatcctgg tcgtcgtcaa gccgttctga caggtcaggc ggcccgtaac
     9601 ctactgtttt taaaagggct tgcaaagtca ccagaccggt tctattctct tgccgcaaat
     9661 gagaagcgtt cttatttgag gtctcgaatg ccccctactc atataaaaaa ggcgcccccg
     9721 gctgcctcgc cgccttccgc cgcacctccc gcgccggaac gcaccgtctc caccaaagaa
     9781 cttctcggcg gaggccatcg cctctggatc gagcacgccg ccgagcgtta cctgctgcag
     9841 gtcacgcgca gcggcaagtt gatcctgacc aagtaacccg ccagactaac tacttccacg
     9901 cccaggtcag ccacccaggc attgccgctc cctcgtgcaa tgatccccag gcagccaacc
     9961 gtcgagcgcg cgccgcgcgc catacgaggt tggcatgaca tttccctcgc agttcgaatc
    10021 gaatgacccc gatgcctggc atgcgctcgg catcgcactt gctgggattg gcgatcgtgt
    10081 cggcgccttc acggcgctgc gcatggcagc gctgctcgat ggcacccgcg caagcactca
    10141 acttgcactt ggaaacctgt tcttcgacac gggtcgcctc gacgatgcgc tccgctgctt
    10201 cgaatctgct gcggcacacg atccgactcg atctcacggc gaaacatgac ttcctctccc
    10261 ggcgcggagt ctggtctccc ggaggctctg agttccatta cgatccgcct caagcaaccc
    10321 ttcgaggaga tcgagaatgc gcgcctccga gtctcttacg gccacgagcc tgattgtcgc
    10381 ggcactattg acgggctgct cgcgcgagcc ggcggaaaag cccgcagaag caaccgcacc
    10441 ggcagacgcc gtgcaggtgg cggccgaatg gccagcattc gtcgagtcgt tcatcgaggc
    10501 acgcttcaat gcggacccgc actttgccgt gcaggcgggc cgccacgaat tcgacggcaa
    10561 gatgccggat ttgagccgcg ccgcgatcga ccgcgaggtc agcgaccttc gcaggtttgc
    10621 cgcagaactt ggcaagcgaa atccggcctc gctgactgcc gcgcagcgat tcgagcacga
    10681 gttcctccag tgggtggtgg atacgcagct cttctggctc accgaggccg acgcgccatt
    10741 ccgcaatcca gcctggtaca tggaaaagct ggatccgtcg atgtatctca cgcgcgagta
    10801 cgcgccgttg ccaaaacgcc tcgagggctt tctgggctat gcgcgtgcgg tgccagcgct
    10861 cgctgcgaat atccgcgcca atctgcgcac accgttgccg aaagcgttca tcgaccgcgg
    10921 caccgcgggc ttcggtggct acgccacgtt ctttcgcagc gagatgcctg ccatctttgc
    10981 gcaggttgcc gatgaagaac tgaagcggga tctcaccgag gcgacggccg cagcggcgac
    11041 ttcgatggat gaactgacag cgtggctcga atcccagcgc gcgacggcca ccgacgactt
    11101 cgctctgggt gcacccctgt ttctcgccat gcttcgtcag accgaacgcg tcgacattcc
    11161 gctcgaggag ctcgagctgg tcggccgcaa ggatctggaa cgcaacctcg cggccctcaa
    11221 ggacgcctgc gcagcctttg cgccgaaagc gagccttgcg gcgtgcgtcg acagaatgcg
    11281 cgctaacaaa ccttcgggtg gcagcgtcga aggcgctcgc gcgcaactcg aggacctgcg
    11341 ccagttcgtg atcgacaaga aactggtctc cattccgggc cgggagcagg cgctcgtggc
    11401 cgaatcccct ccgtacaacc gcggcaattt tgcctacatc agcattcccg gaccgtacga
    11461 gagcccggcg gtgaaggtga cgtactacgt ggccccgccc gatgccaagt ggagcgccgc
    11521 cgagcgcacc gcctatcttc ccggaaaggc ctatcttctc ttcgtatcgg cgcacgaagt
    11581 gtggcccggt cactacctcc agtcgcagtt catgaacgcc aatccatcgc gtgccgaggc
    11641 gttgtggtgg aactattcat tcggcgaagg ctgggcccac tacgccgaag agatgatgta
    11701 cgacgcgggc ctgggagctg gccagcccga aatgcgcgtc ggcatgctga tcaatgcgct
    11761 gatgcgcaat gtgcgcttcc tgtcggcgat ctgtctgcac agcggctgca tgtcgctcga
    11821 ggagtcggag aagatgtttc gcgatagcgc cttcaccgac gccggtaatg cacgccaaca
    11881 ggcgctgcgc ggcacttacg accctggtta cctggcgtat acgctgggca agctcatgat
    11941 ccgcaagctg cgggcggact ggctcgcggc gaatccgtcg gcgaccccgc agcaattcca
    12001 cgacaaattc ctgtcgtttg gtgcgcctcc cgttccgttg gtgcgcaagg aaatgctcgg
    12061 aagcaccgga gcggtgctct gatctggtgc ctaactacac agtttgtgat tttgtgtggc
    12121 gaagcgtcat gttgtgtgca aaaaatcccc atcgacgcac cgcgatcaag cagaaaaagc
    12181 gccgtttttt gtcggcgccc accgacatcg actgttcatc aacgctgagg gaacttctta
    12241 cgccaaatgt gtgacatacc cacatgggca aaaacaaaat ggtcttttcc gcggcggcct
    12301 gcgccgcgat ggtgggagtg gcgctcgcac aagttcaagc gagcagcacg gtgtatctga
    12361 tcgacgttga tccggcgcgt tacgcgggcc ccgcgggcca gcgtgtcatg ctgcgcttgg
    12421 gcgatcagcc gaggaacgtc accatcggca cgcaggacga aatatgtgtg cagttgcaac
    12481 gcgtcgaagg tactgaagtc atcagcgcac gcgttcgcgc gttgcggcct gagccgaaga
    12541 ctgcggctga gattccgttg gttggtccga acatcgcgcc aggaacgtat cgcagcacgc
    12601 catggggatt gctgcttcgc gcaatgcccg ggactactga cgcgcaggct ttcgtgaaag
    12661 cgttcggtac cgacaactcg ccggtctgca ccctctagct ccggggtcaa tccttgagca
    12721 gcgagtcgat gatgcggctt tcgttgtcgc gtccgccgcc ttgcgcgcag acttttcggt
    12781 agagctcggc ggcaacactt ccaaacgggc aatccacgcc aagctttttt gcgaggcgca
    12841 acgcgtactc gatgtctttc aggcgcagcg cgggcgtgaa atggacgttg gttccgtggt
    12901 ccccggccac gaatcgtcgc acgttgcgca cgacctgcgg gctggcggct tggccgtttg
    12961 cgacggcatc ggctacgagc ttcaggtcga gacctgcccg cccggcgagc acgacggctt
    13021 cggccgccga ggcaatctgt accgcgccca gcaggttgat catcagcttg tacaccgtgc
    13081 cttggcccac ggcaccgaag tgcagcacgc gcgtcgcgag cgacgcgaag atcggccggg
    13141 cggcatcgag gtcggcggcg tccgcaccga ccagcagcgt cagcgtgccc gtcgcggccg
    13201 catcgggcag gccggtgaca ggcgcatcca cgaagcgaaa tcgccgctcg cgcacacggt
    13261 cattgagctc gagcacccag tcatgcgaga gcgtcgaaca ctcgatggca agcgcgtgcg
    13321 gtacgttgtc ggcggccagc gcaccctgat tgcccagcca catggctcgc gacgaatcgt
    13381 cgtcggccgt cataccgatg attacgtcgg ccttttgcgc cgcagcgctg ggagactccg
    13441 ctatgcgtgc gccgagtttc gcaaatggtg cggcacgctc cgccgtgcgg ttgtacaccg
    13501 ccagcgaatg acccgccgcg agcatgcggg ccgccatacc ggcgcccatc ttgccaagtc
    13561 cgaggaatgc cacgcgcatc agttgaaccc cagtttccgt ttgaccacgg agttgtccac
    13621 cgcgcgaatc ccgcgagggt cgagccgcca tttgtccatg tattgcaccg gcacgatgcg
    13681 gcgcactccc tgctcggtga gcagtgccgc gagttccggg tcgtcgtagg gctggagcgg
    13741 gccggccttg aaggggccga acaacccgtc ccaatggacc ggtagatagg ccttcggcct
    13801 gaggacgggc agcacgagcg ccgccagttc gcggccgccg gtggcgatcc acaggtcgac
    13861 ggcgtcgatg ccggcggcct tcatggccgc gatcaggttg tcgagcggcg cgccgtagtc
    13921 cttgccggcc aggaccaccg gctcgcgcag gtcgtcgggg ctgccggagt cctgaaagaa
    13981 ccagctgaag cgcccctgcg gcccatcgac catgaataga taggcgcgat tgccaccgcc
    14041 gttcggaaaa tcctccagga cgccggcacg cagggcgccg cgggcatcgg ggaccgggag
    14101 tccctcgagc tcgcgcggtt catgaagctc ggggttctgc gccggcccgc cactgtgatt
    14161 ccagcggata acgtacacgt ggacgccgcg ctcgagttcg aatacttcac cgccgatcac
    14221 cggagtgcag cggctcgctg caatcccttg cgcgcgcgtt tgcaggcagg cggtcggcgc
    14281 accgatcacg cgcgctccgg ataacttcgc ccaggtgccg gtatcgaacg agtgatcgaa
    14341 atggttgtgg cccgtgagca ggaggttcac cgcgggtttg ccaccaaggg catccagcac
    14401 ctccttcacc gccgcctcgt ccggccgcgc cgctagccgc gtcttaccgt agccgcttgc
    14461 gccgctgtag aagatctccg gtggcaggcg cgtgatgtaa ccgtccgcga caattttctg
    14521 gctgcccaga tcgaagtgca tgttggcgat cgacatccag gtgatgtcga cgtggccggg
    14581 tgcggcttgc gcgtgcgcgc cgcccaccag tgtggcgcag gcgacgagcg tgcggaacag
    14641 ttgaatcatg tcggctccat cgaggagccg acatcataac ttcagcgggc cagccagccc
    14701 ccatcgaccg ggaggatgat gccctgaacg tagttcgcgg cgctcgatgc caggaatacc
    14761 gcggcgccac ccaggtcctt cgggtcgccc cagcgtccag agggaatgcg tccgagaatt
    14821 tcagcgttgc gcttcacgtc ggcctgtagc gccgccgtgt tgttcgtcgc gaagtatccg
    14881 ggtgcgatcg cattgacgtt gatgccgctg acagcccact cgttggcgag caacttggtg
    14941 agcccggcga tgccgctctt gctggcggtg taggagggca cacgtatgcc gccctggaac
    15001 gacagcatgg aggcaatgtt gatgatcttg ccgccaccgg cgggaatcat gtggcgcgcc
    15061 gccgcctgcg acaggaagaa gaccgacttc agattgacgt cgagcacggc gtcccagtcg
    15121 gcttcggtga aatcgatggc gttgttccgg cggatgatgc cggcgttgtt cacgagaatg
    15181 tgcagcccgc cgagcgcacc gacaatgtcc gcgacgatgc gctggtagtc gggttttgcg
    15241 ccaaggtccg cgttcaccca gtggccgcgt ctgccgaacg atcgcacggc ggtaagtgtg
    15301 tcggcagggt cgctgcgacc gatgcacgcg acgtcggcgc ccgccgctgc gagcgcttcg
    15361 gcgatcgcgc gaccgagacc ggtattcgcg cccgtgactg ccgcgacgcg gcctccgaga
    15421 tcgaaggatc gatcgaagct gttcgccatg atcaacgcaa cacgttcatg gggacccagg
    15481 cgacgtcttt gtattcctgg ttttcgccgc ccatggccca gatgaagctg tagttggtcg
    15541 tgccgcagcc ggtatggatg ccccacggcg gcgaaatgac ggcttcttcg ttggcgacga
    15601 agatgtgccg cgtttcatgc ggctcaccca tgatgtgcac gacgcgcgcg tcgggcttga
    15661 ggtcgaagta gaagtacgtc tccgcgcggc gctcgtgcag gtggcagggc atcgtgttcc
    15721 acacgctgcc ttccttgagc gacgtcaggc ccatgagcag ctgcgccgac ttgcagatat
    15781 ccgggttgat gtattgatag atggtgcgct cgttggccgt ggcggcggcg ccgaccggca
    15841 tcgggttcgc cttcgcaatg tcgatcttca cgagatcgta tcgcgcgtgc gccggtgtgc
    15901 tggcgagata gaacttggcg ggagcgtcct tcgaatcgga cgagaacgac aatgcggtgg
    15961 tgcccatcgg cacgtacaag ccttcgcgcg ccgcgagcgc cgtggtctgg ccgtcgacga
    16021 tgaccttgcc cggaccgccg acgttgatga tgccgagctc gcgccgcgcg aggaacgggc
    16081 ccttgttgag cgccggcact tcgttgagct cgatggtctt cgacacgggc atgacgccgc
    16141 cgttgatcat gcgttcgatg tggctgaacg tgaggttcac gtcatccgga gtgaacatgg
    16201 cgggtatgtg gtagagctca cgcagcttcg cggtgtccgc ggtcgcgatg atgctcggat
    16261 gacttgcgtg tctcgtgtcg aacttcatga ttctctcgtt cggggtcttt gctggggcgg
    16321 cgattctacc ctgacttccg gcggcgcggt aaccggtgtc attcgggcgt ggcacgccat
    16381 gaattgttaa gacgggcggg gacttatcgc ggaggcggct ggcaggagtc gcggatgacg
    16441 aggtgcggct cgagcggggt caccatggcg tgcgtcccac ctgcggtttc cggatgcagg
    16501 atcttgttcg cagccaggcg tcccagctca cgaatcggca ggcggatggt ggtgagcgag
    16561 ggcagcagcc gcgacgccag cggtccgtcg tcgaagccga cgatggagag gtctccggga
    16621 atcgacagcc cgagacgatt ggccacgcga tagacgccgg ccgccatttc gtcgttgctg
    16681 gcgaaaatgg cggtggggcg cgggctcagg gccagtaact tctcgccccc ggccacgcct
    16741 gattcgtagg tgtaaccgcc ttcgacgatg aggtcggaag gaaaaggaac gccgcgcgcg
    16801 accaaggctt cgcgaaatcc cgcgagtcgt tcgacggcgg aacggaatcc cgggggaccg
    16861 gcgatgtagc cgatgcgctt gtggcccagc gaaacgagat agttggccac ttcggcgacg
    16921 gcgagccggt cattggaaat gaccatgcgc gacggatcgt cgagctgggt ataggtgatg
    16981 cgcacgtacg agcaatccgc ggccgtgagc gcgcgcgtga gctcatcgtt ttcggagacc
    17041 ggcggcagca ggattacgcc gcgcagcttc tggcgctcga cgaaacggcg cacgccggtg
    17101 atgaagtcgg gcttcttgcg atcgcagggg tgcacgacga cttcatattc ggaattgcgc
    17161 acggcatcga gcgcgccttc catgcaattg acgatgtact gcgcgttcgg gttgtcgtac
    17221 accagcccga tgaggaaagc cttgcggaag gccaggccgc gcgcctgcgg atccggaacg
    17281 tagccaaact tgtcgatgac cgcctggatt cgcgcgcgcg tggcttcctg cacgagcggc
    17341 gactggttga tgacgcgcga gacggttttt ttcgagacgc tggcaagacg cgcgatatcg
    17401 ttgatggtgg cgttacgacc gggcttgatg ccggtcacct ttgcagggac cgccagcttt
    17461 ggcatgccgc gattgcccgc ggcttttttc cggatgcgtg tcatggtggg gattgtacgg
    17521 aagcgggcgc ggctggggca ggtatgtcct ctaacaatca gcgatctgtc accggtgtca
    17581 tgggcaccgt aacgcatttc aggctgccaa cgccggtgaa gattgcctcg gccgattgtc
    17641 ctgctccgat ggagtggaca cccgtggcgg cgccggtgga gatgtagtcg ccggcgcgca
    17701 gcggccggcc gcggcgcgcg ttacaacgca gcgcgaaggc gagggcggcc agcgggcctc
    17761 cactgacggc agcggcgccc ccgcgaccta cgacgttggt atcgatccgg gtttcacagt
    17821 tcagtgactc gagactgcgc gtctgccagt cggtgacttc ggcgccgagc agcaggcctg
    17881 cgttgttgcc gaaatcgctg gcaacgaccg cgggtccata gtcgttgata ttgaccagcg
    17941 ggctgctcgc gatctcgatg ccgatataca tcgctttgac aaaacgtcgc gcggtttcgg
    18001 tcgtccattc cgtgacgttg gccggggcgt ctttggccag ctggaccacg aattcggcct
    18061 caatcgcagc gaatccgttg gcgaatacgg gcacttcgat ggaggcgctg ccattggcat
    18121 gtttgaccga gccgctgaag atgggtccca caagtcgctg tgcgccatat ttatggctga
    18181 gcggctcggg tatccaaccc actttccagc ccgctatgga gtcgtcccaa agcgagatgg
    18241 cctcgtcctg gcaacggtat gccgaatcga ggtcttgggg ctgggtgccc gggtattgcg
    18301 taaacgagcc ggccgaacgg cgggccttta cgaattcggc ggccacccct ttggcgtcga
    18361 acgcgaggtc ggaaacaacg gaaaccaatt ttatgaacct tctttattca gcgctcggtt
    18421 gcagcggacg ttatataggg aaaccggtgt cattttcaat gcttcggagg gacatttcat
    18481 gtcatatcgt cgggatttgc cagacattat tgttcgcgta aatggctttt gccgctgaga
    18541 cgccactccc agttccgggg atccaatggg gttgcaccta tgtcccctgc tgaatgaagg
    18601 gcactttggc gaacagctgc cgttcgtttc cgcgcgggtc gtcgacgagc ctgctttcta
    18661 cgttgaaaac catggtggcg cggcggggca gcgcgtaggg ttcccagcgc ggcagaccct
    18721 ggtagttagg atcgccattt cggcagaacg ccagtagcgc ctgctgcatg gctgacgaca
    18781 cgcgccgcgc ttcctcgccg tcgccggtca gggactcgcg cgcggtcaaa gtgccgaaca
    18841 tcagcgggat gtccagcgtg tggaacgcgc cccacttgcc accgtcctgc ggcgagcccc
    18901 agtcgagctg ataggcgaac gccggggagc cctgcgcggc gcgcagttcc gcctcgacga
    18961 tggc
//
LOCUS       DASQJS010000021        10926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4410306, whole genome shotgun sequence.
ACCESSION   DASQJS010000021 DASQJS010000000
VERSION     DASQJS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4410306"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..409)
                     /locus_tag="VEW08_01020"
     CDS             complement(17..409)
                     /locus_tag="VEW08_01020"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HYJ39334.1"
                     /translation="MTRTQRRLIRFAVAATGLLPVLALAHPDHGETSSFVAGALHPLG
                     GIDHLLGLITVGLLMSRLGGRYLPLLAAAMLGLLVAAWTVDSDGSLYAAGFMVTSAAL
                     IAAAMAATRTATRLLGLATTAIAPRSPT"
     gene            517..1134
                     /gene="ureG"
                     /locus_tag="VEW08_01025"
     CDS             517..1134
                     /gene="ureG"
                     /locus_tag="VEW08_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HYJ39335.1"
                     /translation="MSSRSDVLRVGVGGPVGSGKTALVDSLCKRMRDAFNIAVVTNDI
                     YTKEDQEFLIRSQALAPERIRGVETGGCPHTAIREDASMNLAAIDQLMHDFTGLDFII
                     VESGGDNLAATFSPELSDLTIYVIDVAAGDKIPRKGGPGIMKSDLLIINKIDLAPHVG
                     ASLEVMDRDAKRMRGDRPFVFTNLKTGQGLDQVVAFIEKQGMLRH"
     gene            1235..1714
                     /locus_tag="VEW08_01030"
     CDS             1235..1714
                     /locus_tag="VEW08_01030"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HYJ39336.1"
                     /translation="MQGPPFRVLFVSQRNSARSMMAEAIANAIGHGEFEAYSAGVRPA
                     AHIDPMAVELLKHAGIEAPDHPPQHLRSFSAPGAPPLDFVFTLSDTAAGEAPPSWPGQ
                     PFTAHWRCDDPEKYETEAERRMSLLRVRTELERRLRLLTNLPVASLDRLSLHKHLGN"
     gene            1820..2842
                     /locus_tag="VEW08_01035"
     CDS             1820..2842
                     /locus_tag="VEW08_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HYJ39337.1"
                     /translation="MTAMRRLGFGLGLLSLLALPAIAQEPAPDPMDEVVVTGEFPGPG
                     MWKVTRAEPGHVLWIVGEPPPLPKRMKWKSKGIEAVALSAQEILRDSAVNMEPDEKIG
                     FFRGMTLLPAILEARRNPDDAKLKDLVPADLYARWLVQKKLYLGRESGVESWRPLFAA
                     DKLRKAAFDDLKLREGGAVWDVIGKLAEKHKIKVTSPMLRFTIKRSEVRTKIKEFSRE
                     SLSDVECFATMLALTEALGDRETQNARAHAWAVGDHEALAAIPALPSPYLPCAMAVMS
                     SQVARELVPADIREKVHALWLEAAEKSLAANQTTFAIVPFVKLTRPGGYLAMLRDKGY
                     LIEAPQ"
     gene            complement(2823..3503)
                     /locus_tag="VEW08_01040"
     CDS             complement(2823..3503)
                     /locus_tag="VEW08_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39338.1"
                     /translation="MRHFACIFVLGLALVAGCAADRPPEEMTDDGLVRVPSRSAGGVY
                     RAPDVSFLPYRRIILEPPSISFVKDWLENHPEVSPAEFARIRAESVQMFRDEFAREFV
                     ERGPYKFADDPAPDVLLVIPAIEDLNIMAPDAGVAPGERTYTSGPLSMKVSGDLRDAL
                     TGRIVGRVTMYQPPDRYAFNEMRIANRVTNAHEQRLAFAKWSRLVREALNVAKAERPR
                     APATVAPR"
     gene            3816..4106
                     /locus_tag="VEW08_01045"
     CDS             3816..4106
                     /locus_tag="VEW08_01045"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HYJ39339.1"
                     /translation="MSRRAFHKFRLYIAGSAGNSALAIQNLDSICRAYLPGQYEIEHV
                     DVFKEPERARADSIVMTPTLLRLSSKPVCRVVGTLSDSRSVLTVLGLAAAAA"
     gene            4103..7840
                     /locus_tag="VEW08_01050"
     CDS             4103..7840
                     /locus_tag="VEW08_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ39340.1"
                     /translation="MIAAASSSLVDTEKEIASLIERLRDTEQRLEELTAGEVDSVASS
                     DGRVFLLRHAQRNLRESEGAKQAAMLNALSAQVEAHRLAEDALRASDQRFRQMAENIQ
                     DVFFLNEVGTNRVLYVSPAYEKIWGRNCADLYANPQAWMDAIHPEDRSNAQEQYSRGI
                     YGQAFEHEFRIVRPDGVIRVIEARGFPVSDAEGNTIRIAGVAEDVTERNRLEQAVNES
                     ERHFHFLDDLAQATRALSESGQVMTVMARMLGEHLNASRCAYADVERDGQRFRIKHDY
                     TDNCASTVGHYELGLFGARALQTVTSGQTLVIRDVKGELSSEVGAEMFAASGIEAIIV
                     CPVVREGRLRAMMAVQQTTPRDWSASEIAIVQEVVERCWSAIERRSAEEKQREGEALL
                     RIAGSTAHMGGWAVNAADHSVTWSDEVCHMLEVPPGTVPELDQALNLYPMKSRSRIAN
                     SLEACLLGGTAFDLELEMLTAKGRTIWVRCAGEARRNHVGAITHAHGALQDITDRKLA
                     ETALGESERRFSDMLGNVQLVSVMLDAGGLITYCNDFILKLTGWHSSEVMGRNWFDLF
                     VPKDQGELREVFAMSLVEAPQSWHRENEIITRAGERRLIRWNISVLRSATGEVNGTAS
                     IGEDITERKRAEISIKHLNRVYSVLRGINALIVRARDRDELFREACRIAVEAGGFRMS
                     MICLIDQVTKKLVAVASAGKDDELMAAVRGLLASPETFVNTKLSDAIREKKPIVSNDW
                     LRDKGPVLHEKYVQAGVRSLVVIPLIVAGEVTGVMALYAREADFFHEEEMKLLLELAN
                     DVAFAIDHIEKGEKLSYLAYYDSLTGLANRVLFHERLTQRVASARVEHGRVALVLMDI
                     ERFRTINDTFGREAGDALLKELAARMVALVADVGWLARIDGDHFAIMVPGLQEEEMAR
                     RIEQRVVEIFGEPFRVAESELRLSARFGVAMFPNDGTDADALFRNAEAALKNAKASGD
                     RYLFYTNSMNERVAEKLVLENHLREAIEKQEFVLHYQPKIDLASGKLTGCEALIRWND
                     PRTGLVPPGKFIPVLEETGMIFEVGRWALRQALVDGLRWRHAGLPAVRVAVNVSPLQL
                     RNRAFVAELRQLIGVDATAATILELEITEGMIMEDLKNSIATLQEIRAMGVTIAIDDF
                     GTGFSSLSYLARLPVDTLKIDRSFVNDMNSGPVGLSLVSTIITLAHSVKLKVVAEGVE
                     TPEQSRLLGMLKCDEVQGFLYSKPVSGEEFEARFLAPSGAP"
     gene            complement(7862..8794)
                     /locus_tag="VEW08_01055"
     CDS             complement(7862..8794)
                     /locus_tag="VEW08_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HYJ39341.1"
                     /translation="MANTTASGAPDVRRDTILVVDDTPETLGFLTDTLDHAGFTVLIA
                     TDGDSALRLIDQITPDLVLMDAVMPGLNGFETCRRMKRNKMFSGLPVIFMTGLTETEN
                     VLEGLAAGGVDYVTKPLVVDELLARIRVHLANARVAAGTRAALDATGRFLLATNSSGR
                     LLWCTPKARQLLAELFPAAGEHNASLPAATMGQLMQLRAEGGNTRLLVDVNGRKVEIS
                     VLSPIGPDELLFRLTELSTTADEHQLQQTLSLTGRESEVLLWISRGKANREIGEILAI
                     SPRTVNKHLEQIFVKLGVENRASAAARAVRALAR"
     gene            complement(8787..>10926)
                     /locus_tag="VEW08_01060"
     CDS             complement(8787..>10926)
                     /locus_tag="VEW08_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ39342.1"
                     /translation="SPKYIEFKRAHLYDINPVGVGALAISVVLSSAAFMGAFGLYAQI
                     LSPFVALMVAFIAAPLIAWGTKGRYYLARPATGLPENVSELECSICENVFERGDIAMC
                     PAYSGPICSLCCTLEARCRDQCKTDSRFNEQLDGFIRRMLPRRVGALLNTRAGHFGGL
                     LLLSNLAVGLLLGLIYQQYGGSVPEERETIRTTLWLVYFALLVVSGVMAWLIVLAHES
                     RRAAEAESARQTSMLMDEIDAHKRTDAALQKAKEVAEAANVAKTRYIAGISHEIRTPL
                     NSIYGYAQLLERGAAGPSDNAIRVIRRSAEHLADLIDGILDISKIENGILRLNRDKVP
                     LAEFLDQIVDMFRLQAAAKGIEFVYQRPPNLPAFVHVDQKRLRQILINLISNAVKYTE
                     RGSATLTVRYRSQVAEFEVADTGVGIPAHELERVFEPFERGQGVNVRAIPGTGLGLTI
                     TKLLTQIMGGEINASSIEGQGTTFTVRLLLSEAAPAPNAMRDAAEGVRAVISYAGLRR
                     KLLLIDDDPSHIDIVRRLLGPLEFELYTANDGESGVALAEQHQPDLAMIDLSMPGMNG
                     WQVASTLRTMPGLERLKIVIVSANAHEFAPGGAEAAHDAFLIKPIDMQRMLECLAAQL
                     DLRWVYETGAASAREGDLQGALPEHSRHHVDDLYQLGLIGHVRGIQAKLREMETDPTN
                     KPFANRMRTLVANFDLKRYMNVLEGMRKHG"
BASE COUNT         2075 a         3270 c         3583 g         1998 t
ORIGIN      
        1 gtgctgcgat tgccgcctaa gtcggtgagc gtggtgcgat tgcagtagtt gcgaggccca
       61 ggagtctggt cgcggtcctg gttgctgcca tggcggccgc gatcagcgcc gcgctagtga
      121 ccatgaagcc tgccgcgtag agcgagccat cactgtccac ggtccaggcc gccaccaaca
      181 agccaagcat tgccgccgcc aacagaggca ggtaacggcc tcccaggcgc gacatcagta
      241 aaccgaccgt gatgagaccg agcaggtggt cgatgccacc gagcggatgc agcgcgccag
      301 cgacgaagct ggatgtttct ccatggtcgg gatgcgccag cgcgaggacg ggcaggaggc
      361 cggtggccgc cactgcaaaa cgaatcaggc gtctttgcgt tcgtgtcatg tggcaaagcc
      421 taccgcatgg gcgcagagcc ggactctcgt ataagtcatt tgacgcaggt cgctggcgcg
      481 cgcgcgcgcc gcatccggga cgtatcattg ccgcctatga gttccagatc agacgtattg
      541 cgggtcggcg tgggtggccc ggtgggttcg ggcaagacgg cgctcgtgga ttccctctgc
      601 aagcgcatgc gcgatgcatt caacatcgcc gtcgtcacca acgacatcta taccaaggaa
      661 gaccaggaat tcctgatccg cagccaggcg ctcgcacccg agcgcattcg cggcgtcgaa
      721 accggcggct gtccgcacac ggcgattcgc gaagatgctt cgatgaatct ggccgccatc
      781 gaccagctca tgcacgattt caccgggctc gatttcatca tcgtcgagag cggcggcgac
      841 aacctggccg ccacgttcag ccccgagctc tctgatctca cgatatacgt catcgacgtc
      901 gcggccggcg acaagattcc gcgcaagggc gggcccggca tcatgaagag cgacctgctc
      961 atcatcaaca agatcgacct ggcgccgcac gtcggggcgt ccctcgaggt catggaccgc
     1021 gatgcgaaaa ggatgcgcgg cgatcgcccg ttcgtattca caaatctgaa aaccgggcag
     1081 gggctcgacc aggtggtcgc cttcatcgaa aagcagggca tgcttcggca ctgacaggaa
     1141 gcagcagcgg ccgctgacaa ccgaatttcg ccgtggcgat tccggcacac catggttagt
     1201 tccagactcg gcggaaggac cagaggagct gaacatgcag ggcccaccat ttcgcgtgct
     1261 gttcgtatcg cagcgaaact ccgcgcgcag catgatggct gaagccatcg ccaatgccat
     1321 cggccatggt gagttcgagg cgtatagcgc cggcgtccgg cctgccgcac acatcgatcc
     1381 gatggccgtg gaactgctga aacacgcggg catagaggcg ccggaccatc cgccgcagca
     1441 cctgcgatcc ttcagtgctc cgggcgcgcc gccgctcgat tttgtcttta cattgagtga
     1501 cacagcggct ggcgaagcac ctccgagctg gcctggccaa cccttcacgg ctcactggcg
     1561 ttgcgatgat cccgaaaaat acgaaacgga agcggagcga cggatgtcac tcttgcgcgt
     1621 gcgcaccgaa ctcgaacggc ggctgcgcct gctgaccaat ctccccgtcg cgtcactgga
     1681 ccggttgagc ctgcacaagc atctcggcaa ctgagcgcgc aagcccgcgc gcgctcttcg
     1741 cgcagacgtg atcaaatccc ggaaccttgg gcacttcgcg cgctcatagg taggtctcca
     1801 cgcgctaaca cgggaagtca tgacagcaat gcggcgcctt gggttcggtc tgggtttgtt
     1861 gtccttgctt gctctgcccg ccattgcgca ggagccagct cccgatccca tggacgaagt
     1921 ggtcgtgacg ggcgagtttc cgggacccgg aatgtggaaa gtgacgcgcg ccgaacccgg
     1981 ccacgtcctg tggatcgtcg gcgaaccgcc gccgttgccc aagcgcatga aatggaaatc
     2041 gaagggcatc gaggcggtcg cgctcagcgc ccaggaaatc ctgcgcgatt ccgccgtcaa
     2101 catggagcct gacgaaaaga tcggcttctt ccggggcatg acgctgctgc ccgcgattct
     2161 cgaagcgcgt cgcaaccccg atgacgccaa actcaaagac ctggtgcccg ccgatctgta
     2221 cgcgcgctgg ctcgtgcaga aaaaactcta cctgggccgt gaaagcggcg tggaaagctg
     2281 gcgcccgctg ttcgccgcgg acaagctgcg caaggccgcg ttcgacgacc tgaaattgcg
     2341 cgagggcggt gcggtctggg acgtcatcgg caagctggcc gagaaacaca agatcaaggt
     2401 cacctcgccg atgctgcgtt tcacgatcaa gcgcagtgaa gtgcgcacca agatcaagga
     2461 gttctcgcgc gaatcccttt ccgacgtgga gtgcttcgct accatgctgg cgctgaccga
     2521 agctctcggc gatcgcgaga cgcagaacgc gcgtgcccac gcgtgggcgg tcggcgacca
     2581 cgaggccctg gcggcaattc cggcattgcc cagtccctat ctaccctgcg ccatggcggt
     2641 gatgagctcg caggtcgcgc gtgaactggt gccggccgac attcgcgaga aagttcacgc
     2701 cctgtggctc gaggccgccg agaagagcct ggcggccaac cagactactt tcgccatcgt
     2761 gccgttcgtg aaattgacgc gccctggtgg ctatctcgcc atgttgcgag acaagggcta
     2821 tctcatcgag gcgccacagt agcaggcgct ctcggccgtt cggccttggc cacgttcagc
     2881 gcttcgcgca cgagccttga ccatttcgcg aatgccaggc gctgttcgtg cgcgttggtc
     2941 acccggttcg caatgcgcat ctcgttgaag gcataacgat cgggtggctg gtacatcgtc
     3001 acgcgaccga cgatcctgcc ggtcagcgcg tcgcgcagat cgccgctcac tttcatcgac
     3061 aacggcccgc tggtgtaggt gcgctcaccg ggcgcaactc ccgcatcggg ggccatgatg
     3121 ttcaggtcct cgatggccgg gatgaccagc agaacgtcgg gcgcggggtc gtcagcaaac
     3181 ttgtacgggc cgcgttcgac gaactcacgc gcaaattcat cgcgaaacat ctgcacggat
     3241 tccgcgcgga tgcgggcaaa ttccgccggc gagacttccg gatgattctc cagccagtct
     3301 ttcacgaagc tgatcgaggg aggctcgagg atgattcgcc ggtacggcag gaagctgaca
     3361 tcaggtgcgc ggtagacacc gccggcgctg cgcgagggga cgcgcaccag gccgtcgtcg
     3421 gtcatttctt ccggcgggcg atcggcagcg cagccggcca cgagggccag gccgaggacg
     3481 aatatgcacg caaagtgacg catgacgcat agcttaacgc tgccgcgatt ggcacgaaat
     3541 gggtgcgccg ccgccccggt tccggctccg gctgtcgacg ccagagccag gtcgaggacc
     3601 ggcgcggcgg ccagcctaac tactgcgtca tcgttcgatg ttggcgcgcg agcctcagcc
     3661 ctaagggtta gtgcgcagtc ctacgtgacc caccgaacat actttgatca gcacgcggcg
     3721 catgatgccg gcccgtacca tgtgcgtcta accacgcgac acgcttcctc caggatttcc
     3781 gtgcaagcac cgcgcgagca aaaaatggcc aatcggtgag ccgacgggct ttccacaagt
     3841 tccggctata catcgcgggc agtgcgggca attctgcgct cgcgattcag aacctcgatt
     3901 cgatctgccg cgcctacctg ccgggacaat atgagatcga gcacgtcgac gtgttcaagg
     3961 aaccggaacg tgcgcgtgcg gactcaatcg tcatgacgcc gacgctcctc aggctctcat
     4021 cgaaacccgt ttgccgggtg gtcggcacgc tgagcgactc gagaagcgtg ctgactgtgc
     4081 tgggcctggc agccgccgcc gcatgatcgc cgcggcttcc tcgtcgctgg tcgatacgga
     4141 aaaggaaatc gcgtcgctga ttgaacggtt gcgcgacacc gagcaacgcc tcgaagagct
     4201 gactgccggc gaagtggact cggtcgcgag cagcgatggc cgcgttttcc tgctgaggca
     4261 cgcacagcgg aatctgcgcg agagcgaagg cgccaaacag gcagccatgc tcaatgcgct
     4321 ttccgcgcag gttgaggctc atcggctcgc cgaggacgca ctgcgcgcca gcgaccagcg
     4381 tttccgccag atggccgaaa acatccagga tgtcttcttc ctcaacgaag tcggcaccaa
     4441 tcgggttctc tatgtgagtc cggcctacga aaagatctgg ggccgcaatt gcgcggattt
     4501 gtatgcaaac ccccaggcct ggatggatgc catccatcct gaagatcgct ccaatgccca
     4561 ggagcaatat tcgcggggaa tttacggcca ggcgttcgag cacgagtttc gtatcgtgcg
     4621 tcccgatggc gttatccgtg tcatcgaggc gcgaggattc ccggtttccg atgccgaggg
     4681 gaacacgatc aggattgcgg gcgtcgccga agacgtcacc gagcgcaacc ggctcgagca
     4741 ggcggtcaat gagagtgaac ggcactttca ctttctcgac gatctggccc aggcaacgcg
     4801 ggcactcagc gagtccgggc aggtgatgac ggtgatggct cgcatgctgg gggagcatct
     4861 gaatgcctcg cgctgtgcct acgcggatgt cgagcgcgat ggtcagcgat tcaggatcaa
     4921 gcacgactac accgataact gcgcgagtac cgtggggcac tatgagctcg ggttgtttgg
     4981 tgcccgcgcc ttgcaaacag tgaccagcgg gcagacgctg gtcattcgcg atgtaaaggg
     5041 ggagctctcc tccgaagtcg gtgccgaaat gtttgcggcc agcggcatcg aggcaatcat
     5101 cgtctgcccg gtggtcaggg aaggacgact acgcgccatg atggccgtgc aacagacgac
     5161 accgcgcgat tggtcggcca gcgaaatcgc gatcgttcag gaagtcgtgg aacgctgctg
     5221 gtccgcgatc gagcgaaggt ccgcggaaga aaagcagcgc gagggtgaag cgttgttgcg
     5281 catcgcgggg agcaccgcac acatgggtgg ctgggcagtc aacgccgccg accatagcgt
     5341 cacatggtcc gatgaagtct gtcacatgct cgaagtgccg cccggcaccg tacctgagct
     5401 cgaccaggca ttgaacttgt atccgatgaa gtcgagatcg aggattgcga attcgctcga
     5461 agcctgcctg ctgggcggga cggctttcga tctcgagctg gaaatgctca ccgcgaaggg
     5521 caggaccatc tgggtgcgct gcgcgggaga agcacggcgc aatcacgtgg gagcaataac
     5581 gcacgcgcat ggcgcgctac aggacatcac cgacaggaag ctggcagaga cagcgctcgg
     5641 cgagagtgag cgtcgtttca gcgacatgct gggcaatgtg caactggtgt cggtcatgct
     5701 cgatgcggga gggctgatca cctattgcaa cgacttcatc ctgaaactga ccggctggca
     5761 ttccagcgag gtgatgggtc gaaactggtt cgacctcttc gtgccaaagg accagggtga
     5821 gctgcgcgag gtgttcgcga tgtcactggt cgaggcgccg caatcctggc atcgcgagaa
     5881 cgaaatcatc acgcgagcgg gcgagcgccg gctgattcgc tggaacattt cggtgttgcg
     5941 ctccgcgacc ggcgaggtga atggcaccgc gagcattggc gaggacatca ccgagcggaa
     6001 gcgcgcggaa atcagcatca agcatctgaa ccgcgtgtac tccgtgctga gaggcatcaa
     6061 cgccttgatc gtgcgggcgc gcgatcgcga cgagttgttc cgcgaggcct gtcgaatcgc
     6121 ggtcgaggcg ggcggctttc gaatgtcgat gatctgcctg atcgaccagg tcacgaagaa
     6181 gctcgttgcg gtggcgtcgg cgggcaagga cgatgagctc atggccgccg tccgcggact
     6241 gctcgcctcg cctgagacat tcgtgaatac caagctttcc gatgcgatcc gggagaagaa
     6301 gcccatcgtg tcgaacgact ggttgcggga caagggcccg gtgctgcacg agaaatacgt
     6361 ccaggcgggc gtgcgctcgc tcgtcgtgat tccgctcatc gtcgccggtg aggtgacggg
     6421 agtgatggcg ctctacgccc gcgaggcgga cttctttcac gaagaagaga tgaagttgtt
     6481 gctggagctg gccaacgacg ttgcgttcgc gatcgaccac atagaaaagg gcgagaagct
     6541 ttcctatctg gcgtactacg attcgctcac ggggctggcg aatcgcgtgc tgttccacga
     6601 gcgacttacg caacgtgtcg cgagcgcccg cgtcgagcac ggcagggtcg cgttggtgtt
     6661 gatggacatc gagcgattcc ggaccatcaa cgacaccttt ggccgcgagg cgggcgatgc
     6721 gttgctcaag gagcttgccg cgcgcatggt cgcgctcgta gcggatgtgg gctggctggc
     6781 acgcatcgat ggcgatcatt ttgcgatcat ggttcccggc cttcaggagg aggagatggc
     6841 acggcgcatc gaacaacgag tcgtggagat cttcggcgag ccctttcgcg tcgcggaatc
     6901 cgagctgcga ttgtcagcgc gattcggagt cgcgatgttt ccgaacgacg gcacggatgc
     6961 cgatgcgctg ttcagaaatg ccgaggcagc gctgaagaac gccaaggcga gcggcgatcg
     7021 ctatctcttc tacacgaaca gcatgaacga gcgagtcgcg gagaagctgg tgctcgagaa
     7081 ccacctgcgt gaggcgatcg agaagcagga gttcgtgctg cactaccagc cgaagatcga
     7141 tcttgcgagc ggcaagctca ccggttgcga agcgctcatc cgctggaatg acccgcgcac
     7201 aggcctcgtg ccgccgggaa aattcattcc tgttctcgaa gaaaccggga tgattttcga
     7261 agtggggcga tgggcgcttc gccaggcgct cgtggacggg ttgcgctggc gccacgcggg
     7321 ccttccggcc gtgcgcgtcg cggtgaacgt gtcgccgctg caactgcgca accgggcttt
     7381 tgttgcggaa cttcgccagc tgatcggcgt tgacgcgact gcagccacga tcctcgagct
     7441 cgagatcacc gagggcatga tcatggaaga cctcaagaac agcattgcca cgctgcagga
     7501 aattcgtgcc atgggcgtca cgatcgcgat cgacgatttt ggtacaggtt tctcttcgct
     7561 gagttacctg gcgcgcctgc cggtggacac gttgaagatc gatcgttcat tcgttaacga
     7621 catgaacagc ggcccggtgg ggttgtcgct ggtgtccacc atcatcacgc tcgcgcactc
     7681 ggtaaaactg aaagtcgtcg ccgagggtgt ggagacgccc gaacagtcga gactcctggg
     7741 catgctgaag tgcgacgaag tacaggggtt tctctacagc aaaccggtgt ccggcgaaga
     7801 attcgaagcg aggtttctcg cgccatcggg cgcgccctga cgcgcgcggt cgcgacggga
     7861 ttcatcgagc cagggcgcgc acggcgcgcg cagcggctga agcgcggttt tcgacgccca
     7921 gcttcacgaa gatctgctcc agatgcttgt tcacggtacg tggactgatc gccaggattt
     7981 cgccgatttc acggttcgcc ttgccacggc tgatccacag cagcacctcc gattccctcc
     8041 cggtcagcga cagggtctgc tggagctggt gttcgtccgc ggtggtggaa agttcggtga
     8101 ggcggaacag cagctcgtcg ggcccgatgg ggctcagcac cgagatctcc accttccggc
     8161 cgttgacgtc gaccaacaag cgtgtgttcc cgccttcggc acgcagttgc atgagctggc
     8221 ccatcgtggc ggccggcagg ctggcgttgt gctcaccggc agccggaaag agttccgcga
     8281 gcagctggcg cgccttcggc gtgcaccaca gcagacgccc cgaagagttg gtggccagca
     8341 gaaagcgccc cgtggcgtcg agcgcggcgc gggtgccggc tgcgaccctc gcattcgcga
     8401 gatgcacacg aatgcgtgcc agcagttcgt ccacgaccag cggcttggtg acgtaatcca
     8461 caccgccggc ggcgagtcct tcgagcacgt tttcggtttc ggtgagaccg gtcatgaaga
     8521 tgacaggcag cccgctgaac atcttgttgc gtttcatgcg tcgacaggtt tcgaagccat
     8581 tgagtccggg catgacggca tccatgagga cgagatccgg cgtgatctgg tcgatcaacc
     8641 gcagcgcgct gtcgccatcg gtggcgatca gaaccgtgaa cccggcgtga tcgagtgtgt
     8701 cggtgaggaa gccgagagtt tcgggagtgt cgtcgacgac caggatggtg tcgcggcgca
     8761 cgtcgggtgc gccacttgcg gtggtgttag ccatgtttgc gcattccttc gagcacgttc
     8821 atgtagcgtt tgagatcgaa atttgcgacc agcgtgcgca tgcggttcgc aaaaggcttg
     8881 ttggtggggt cggtttccat ctcgcgcagc ttggcctgga tgccgcgcac gtggccaatg
     8941 aggccgagtt gatagaggtc gtcgacgtga tgacgtgaat gctcgggcag ggcgccctgc
     9001 agatcacctt cgcgagcgga tgctgcgccg gtctcgtata cccaacgcag atcgagttgg
     9061 gctgcgaggc attcgagcat gcgctgcatg tctatgggct tgatgagaaa ggcgtcgtga
     9121 gcggcttcgg cgccgcccgg cgcgaattcg tgcgcattgg cggaaacgat cacgatcttc
     9181 aggcgttcga ggccgggcat cgttcgcagc gtgctcgcca cctgccagcc attcatgcca
     9241 ggcatggaca ggtcgatcat cgcgagatca ggttgatgtt gctccgccag cgccactccg
     9301 ctctcgccgt cgttggcagt gtagagctcg aactccaggg gcccgagcag ccggcgcacg
     9361 atgtcgatgt gcgaagggtc atcgtcgatc agcagcagct tgcggcgcag accggcgtag
     9421 ctgatgacgg cgcgcacgcc ttcggcggca tcacgcatcg catttggagc cggtgcggct
     9481 tccgagagca ataaccgtac ggtgaatgtc gtcccctggc cttcgatgct ggaggcattg
     9541 atctcgccac ccatgatctg cgtcagtagt ttggtgatgg tgagtccgag gccagtgccg
     9601 ggaatggcgc gcacgttcac gccctggcca cgctcgaagg gttcgaaaac cctctcgagc
     9661 tcgtgggcgg ggatgccgac accggtatcc gccacctcga actccgccac ctggctgcgg
     9721 tagcgcacgg tcagtgtcgc gctgccgcgc tcggtgtatt tcaccgcatt ggaaatcagg
     9781 ttgatgagga tctggcgcag gcgcttctgg tcgacgtgca cgaaagccgg caggttgggt
     9841 gggcgctggt agacgaactc gatgcccttg gcggcggcct gcagccggaa catgtcgacg
     9901 atctgatcga ggaattcggc cagcggaacc ttgtcccggt tcaggcgcag gatgccgttt
     9961 tcgattttcg atatgtcgag gatgccgtcg atgaggtcgg ccagatgttc ggcactgcgc
    10021 cggatcacgc ggatggcgtt gtccgagggg ccggcggcgc cgcgctcgag cagctgggca
    10081 taaccgtaga tggaattgag cggcgtgcgg atttcgtgcg agatgccggc gatgtagcgc
    10141 gtcttggcca cgttggcggc ttcggccact tccttcgctt tctgaagcgc ggcgtcggtt
    10201 cgcttgtggg cgtctatctc gtccatgagc atcgaggtct ggcgcgccga ctccgcctcg
    10261 gcggcgcgcc ggctctcgtg cgcgagcacg atgagccagg ccatgacacc cgacaccacg
    10321 agcagcgcga aatacaccag ccacagtgtg gtccgtatgg tctcgcgttc ttcgggaacc
    10381 gagcctccgt actgctggta tatgaggcct aacaagagac cgacggcgag attggacagc
    10441 aggagcaggc cgccgaagtg cccggcgcgg gtattcagca acgcgccgac gcgcctgggg
    10501 agcatgcggc gaatgaaccc gtcgagctgc tcgttgaagc ggctgtccgt cttgcattgg
    10561 tcgcggcagc gcgcttcgag tgtgcagcat agcgagcaga tcggtcccga gtacgccggg
    10621 cacatcgcga tgtcgccgcg ctcgaagacg ttctcgcaga tcgagcactc gagttcggaa
    10681 acgttctcgg gcaggccggt agcgggtctc gccaggtaat acctgccttt cgtgccccag
    10741 gcgatcagcg gtgcggcgat gaacgcgacc atgagcgcga cgaatggcga gaggatctgc
    10801 gcgtacagcc cgaaggcgcc catgaacgcc gcgctcgaca gcactacgga aatggccagt
    10861 gctcccacgc ccaccgggtt gatgtcgtag agatgcgcac gcttgaattc gatgtacttc
    10921 gggctg
//
LOCUS       DASQJS010000022        14451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4095208, whole genome shotgun sequence.
ACCESSION   DASQJS010000022 DASQJS010000000
VERSION     DASQJS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14451
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4095208"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2113
                     /locus_tag="VEW08_01065"
     CDS             <1..2113
                     /locus_tag="VEW08_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ39343.1"
                     /translation="NTDNTGANFFEAESDEGFWGAKIDWQISDKHLLELLAFSDENQK
                     LSDGYRFDLATAERGVYTGTTYSDDGGVNWAATYTGYLTEDLSVKVLYGENERSSVAG
                     SPADPDCNRILDARGTSTNIGCTNTTQVLDRTDTREAARIDFEWVLGEHQLRFGMDHE
                     TNTSDHVQYYPGADRLLYEIFRVGAAGATVNGVPLAGGTEYVRTRQNETFGAFETINT
                     AFYLEDNWSVTDNLVLNAGVRLEAFDNKTAEGDSYIKIDNMLAPRLGFSWDMKGDGRM
                     KLFGNAGRYFLPVANVINIKQGGAFLDERTFYGLAGFESYEYNGVTYQRPVLGAQFGP
                     VDNSQGDGTVGDFRSEVDADMDPVYQDEFILGFQSMINDKWSYGVRGIFRDLHNAIDD
                     MRILSTGIVCNGRPNRAGFIMGNPGQPLTIFTDTDCNGPGGAQGDNDGWVTVDLANDG
                     WAYFSGPNGGGTYTGTITGFPEPKRTYKALELVLDRAWDQKWALNASYTLAFSEGNAE
                     GPVNSDFNFGDSGRTEAFDDPWVQVGGDGYLPNDHRHAFKLRGTYGLGEHWSFGTNLS
                     AISGRPISAQGVGNPFDGHSFHSFYVCTANCTLPAAQRVYEFNPRGSEGRTPWVYDVS
                     ASVTWRASLGFADAQVRLAVYNLLDDHNAREAEDRYQRTIGGTTCGNASECGVGQHHE
                     FGLATTYQSPRYAQLTFKLDF"
     gene            2167..5886
                     /locus_tag="VEW08_01070"
     CDS             2167..5886
                     /locus_tag="VEW08_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYJ39344.1"
                     /translation="MKTVSLGGSLLALTSAIGLSAPLTLDTHVDIPRTYMREARFDAG
                     TNTELKVDLGKMKRGGLDAAFFVIFVEQGPRTPEGYAAAFAAAERKVSAIEDMVRKYP
                     DRIRLATSSKDVIDNHAAGRLSAMMGIENGFVIGKDLGRLDALYARGARYIGLTHTGH
                     NDICTSSGALKEFGDTPASENVGLSVFGESVVRRANALGMIVDVSHASDACVRDVLRW
                     SAAPIIASHSSARALTNHARNLSDDLMRSIAAKGGTVQIVAYTGFLKLDPARDAAEKA
                     LESETARQAGDAEFDSAKHEYLPAHQQGLARLDKDHPLATLDDYLDHIQHAVKVAGID
                     HVGLSSDFDGGGAVQGWKDASQTRNVTAGLRKRGFTEAQIGQLWSGNVLRVWRDVERV
                     AKSSTVTDKKTIDAIFDAVVAEYHLPGMALGVVEDGKVTYTRTAGELVSGGGKAIDAD
                     TLFKIASNTKAMTTGLLARLVDAGKLQWDDPVVKYLPQFKMSEPWITREMRVRDLLIH
                     NSGLREGAGDLMLWPEPNHFTRADIIAGLAHLKPVQSFRSHYAYDNLLYIVAGEVAAA
                     AGGATYEELVRREIFAATGLKRCQVGEWNRDAVGNVAQPHMWEGDRNLAYARDPETVP
                     AISSAAAGGVRCSLNDMLAWMRMWLDPELRPPGQERPWLSRAQHDALWTPQIPMPMSG
                     RQKRWNNGNFSAYGYGWRLSDVDGVRRVSHTGTLGGMYSAFTLLPEKRSGFVFMINGE
                     GSRARTVLNEALVKQFTAPGRAPSAGWYIEQLKMEDQSSASASGPAHLQLPMRRLASP
                     SSMKPWLGRYRDPWFGEISICERAGHTRFNSVKSPLMAGDVMRVGERLLVDWDDVSGD
                     VEPWLNFAAGKPVTLTLAKVDPDGDFSSDFEDLSFTRIGACVPSPKVAPLSDAAAIAK
                     IDALMGSYAGAVPGASVLVLRDGAPVVRRSYGLADLEQRTPASPETNYRLASVTKQFT
                     AAAILLLAEAGKLGLDDPVRKWLPELPEAAQPVTIRHLLTHTGGLIDYEDFVADDAPQ
                     VHDADVLRLLAKPDRTYFPAGSAYRYSNSGYALLALVVEKASGQRFAAFLHDRIFAPL
                     GMHATVAFEDGVSTVPTRAFGHSAGGEAWTRTDQSSTSAVLGDGGIYSSVDDLAKWDA
                     ALYDNRLLREESRKLAFTPATHTDNPSVDYAFGWRITGETLWHSGETRGFRNVIIRFP
                     ERKLTVIMLTNRNDPEPYSTALAIAQLYLPSAGHP"
     gene            5883..7100
                     /locus_tag="VEW08_01075"
     CDS             5883..7100
                     /locus_tag="VEW08_01075"
                     /inference="COORDINATES: protein motif:HMM:NF017495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HYJ39345.1"
                     /translation="MIHNDAVILGLLAATLGVVFWTTQSAQPFWKRFYKYVPALLLCY
                     FVPALYNSFGLIDGEHSKLYFVASRYLLPATLVLLTLAIDLPATLRLGPKALAMFFAG
                     TLSVVLGGPVALLIWKSLAPASVDGELWRGMSAIAGSWIGGAANQAAMKDVFSIEPNL
                     FGVMVAVDVVFANLWLAVLLWMAANQQRVDRWLKADTTSIAALGEKMERLRLENERIP
                     LLPELMIMLALAFGATGLSHALAVPLASAFAEVEWAVRLSLASEFFWIVVLATTFGLL
                     LSFTRARNLEGAGASRLGSALLYVLIATVGMQMDIGAVLTSPVLFGIGFTWIAFHAVV
                     ILVVARLIRAPAFFLAVGSQANIGGAASAPVVAAAFYPSLAPVGVLLAVLGYALGTYG
                     GWLCGQLMRLVSQ"
     gene            7226..8473
                     /locus_tag="VEW08_01080"
     CDS             7226..8473
                     /locus_tag="VEW08_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ39346.1"
                     /translation="MNTPSSPVSASFSGYQKVVVGMLAFLQFAVILDFMLMAPLGAVI
                     MPALSVTPAQFGTVVSAYAFAAGISGLLTAGFADRFDRKRLLLFFYIGFILGTLWCGL
                     AQSFESLLLARIVTGLFGGVIGSVILAIATDLFPPQMRGRVMGFIQTAFAASQILGIP
                     IGIYLSNQWNWHVPFLALVVLGVIGGLLIAVYMKPVADHLKVPQEHSAYNHLRNTVIE
                     PRYLMSFVSVLFLATGGFMLMPFSSAFTVNNLGISLHDLPIIYLVTGIATIFVGPLVG
                     KVADRFGPLRVFFMGTALTIVMVLIYTHLGVSTLAVVTVVNVVLFIGIFSRMIPFQSL
                     MSQVPAQTQRGSFNAINASISQLAGGMASFVAGHIVQQGADGRLHHYDVAGYVVVGTS
                     LLACFLMWRIQRGISANVAALAH"
     gene            8653..10074
                     /locus_tag="VEW08_01085"
     CDS             8653..10074
                     /locus_tag="VEW08_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HYJ39347.1"
                     /translation="MRRAPIRLLALATLLFALAPCMSIAADVQAWVTTADRSRLLARE
                     SGLSFATRATLANRIDVDAGKRYQPIVGFGAAVTDASAWLIQTRLSETQRAALIQELF
                     GRDGGLGLSFTRISIGASDFSRSHYSLGDTPDGKPDPELEHFSIEPNRAELLPVLRAA
                     LAINPELRIVGSPWSPPGWMKTSGSLVHGRLLPEAQPALAAYLIRFVDAFAAEGVPIF
                     ALTLQNEPDFDPPDYPGMKLEPAERARIIGQFLGPMLAKRTAPPLILDWDHNWDKPES
                     PLAVLADREAARYIAGVAWHCYGGNVAAQTQVHDAHPDKDTYFTECSGGEWDPVFGNS
                     LRWMVRELIIGSTRGWSRGIVLWNLALDENHGPHAGGCGDCRGVVTINSTTGAITRNV
                     EYYALAHASRFVMPGAHRIESTTGIKGLATVAFQNADGSMALIVLNDAKRRRKFSVRS
                     AGASFDYALPAGAVATFVWNPKS"
     gene            complement(10071..11147)
                     /locus_tag="VEW08_01090"
     CDS             complement(10071..11147)
                     /locus_tag="VEW08_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39348.1"
                     /translation="MNTKLLFRAIAGVALCFASAAWAGDTRNVILVIADGVRWQEVFT
                     GADPTLLNDKLRKKYWDEDPSVRRRKLFPFLWETVATQGQIYGNQHKGSVARVTNTRW
                     FSYPGYNEMASGAADPRIDTNEFGPNPNVTVFEWLNTRPGLAGKVEIFGTWATFADIF
                     NGSRSGLPIRAGATLVDANDRSAHGLLLSELYRTTTRIEGDDPYDSFLHVVVREHLRA
                     HQPRVMFIGYGDTDTWQHMGRYDAFLETAHSFDAYLADLWRQLQSSAHYKDKTTLVVS
                     TDHGRGSGPTQWRDHGVDQKGSDAIWIAVIGPDTQPLGERQASVAVTQSQVASTIAAL
                     VGEDFQAFNPNAAPPLREVLGAPR"
     gene            11383..12834
                     /locus_tag="VEW08_01095"
     CDS             11383..12834
                     /locus_tag="VEW08_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYJ39349.1"
                     /translation="MSPFDRRSFLSLAGSAAAGSLLSRSAQAAATRPNILFILADDLG
                     YADLSCYGRGDLRTPRLDRMAREGVRFLQSYSSSPVCSATRAALMTGRYQYRLRVGLE
                     EPLRDTAEIGLPPEQPTLPSLLRDAGYRTSLVGKWHLGLLPKFGPLQSGYESFYGVRG
                     GGVDYYSHVGTGGRKDLWDGDQPLDTQGYFTELLTERALQQLRTFTREHTPFFLSVHY
                     TAPHWPWEPPGDDSESRRIAGRSLFHYDGGTHSVYRQMVEHLDAQVGRLLDALAQSGA
                     ARDTLVIFTSDNGGERFSNTWPFSGRKGELLEGGVRVPTLLRWPRAIRAGATSEQVNV
                     TMDWLPTLLAAAGVAPDPGAPSDGINLLPSLAPGANPVPRKLFWRFKANHQRSVRDGD
                     FKLLKIQNNVFLFNVVEDPLERANLRDRHREVFERLKSEWDQWNAAMLPQLPETLASP
                     PSGSDAPDRYGTSPPPNSVDDVSTWPGALPAKL"
     gene            complement(12869..14374)
                     /locus_tag="VEW08_01100"
     CDS             complement(12869..14374)
                     /locus_tag="VEW08_01100"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HYJ39350.1"
                     /translation="MTGPLIRDIVIVGGGTAGWMTAAALSNHFGRACSIRLVESDEIG
                     IIGVGEATIPHIVSFNRILGIDEDEFLRATRGTFKLGIEFVNWSAIGDRYTHGFGKLG
                     PDLDGLPFHHFWLRGAQAGLASSLGDYSINTAAPRRARFMRPRPDMAGSPLREITYAY
                     HFDAGLYARFLRRLAERNGVERIEGKIVSVQQREPDGFINAVVLDDGSRVAGDFFVDC
                     SGMQGLLIEKTLHTGYEDWSHWLPCDRAVAVPSRSVEPLIPMTRATAHGAGWQWRIPL
                     QHRIGNGHVYSSRFMSQEDATAILTGNLDSEPLAEPRHIPFMPGRRKRVWNRNCIALG
                     LAAGFFEPIESTNIHLIQTAISRLMSLFPRAGFDQAEIDELNTQTRIEYERARDFIVL
                     HYKATERDDTPFWQYCRTMEVPPTLQHKIDLYRSNGRFFREDNELFGELSWVQVMEGQ
                     RIRASGYHPFADLRPLDEVRAFLADTQRVIAACVEAMPTHAEFIARHCAST"
BASE COUNT         2544 a         4707 c         4530 g         2670 t
ORIGIN      
        1 caataccgac aacaccggag cgaatttctt cgaggccgaa tcggacgagg gcttctgggg
       61 cgcgaagatc gactggcaga tttccgacaa gcacctcctc gagctgctgg cgttctccga
      121 tgaaaaccag aagctgagcg atgggtaccg cttcgatctg gccaccgccg agcgcggcgt
      181 ctacacggga accacgtaca gcgacgacgg tggagtgaac tgggccgcga cctacacggg
      241 ctatctgacc gaggacttgt cggtgaaggt cctgtacggc gaaaacgagc gcagctccgt
      301 tgccggcagc ccggccgatc ccgattgcaa ccggatcctg gatgcacgag ggacatcaac
      361 caacatcgga tgtacaaata ccacgcaggt tctggaccgc accgacacac gtgaggccgc
      421 ccgcatcgac ttcgagtggg tgttaggcga gcaccagctg cgcttcggca tggaccacga
      481 gacgaacacg tccgatcacg ttcagtacta tccgggcgcc gaccggctgc tgtacgagat
      541 attccgcgtc ggcgcagcgg gcgccacggt gaacggtgta ccgctggctg gtggaacgga
      601 gtacgtgcgc acgcgccaga acgaaacctt tggcgccttc gaaacgatca acacggcttt
      661 ctacctggaa gacaactggt cggtcaccga caatctcgtg ttgaacgcgg gcgtgcgact
      721 ggaggccttc gacaacaaga ccgccgaagg cgacagctac atcaagatcg acaacatgct
      781 cgcgccgcgg ctgggattct cgtgggacat gaagggcgac ggccgcatga aactgttcgg
      841 caacgccggc cggtacttcc tgccggtcgc gaacgtcatc aatatcaagc agggcggcgc
      901 ctttctcgat gaacggactt tctacgggct cgccggattc gaatcttacg aatacaacgg
      961 cgtgacctat cagcggcccg tcctcggggc gcagttcggc ccggtggaca actcacaggg
     1021 cgacggcacg gtgggcgact tccgcagcga agtcgacgcc gacatggatc ccgtctacca
     1081 ggacgagttc atcctgggct ttcaatcgat gatcaacgac aagtggtcat acggcgtgcg
     1141 cggcatcttc cgcgatctgc ataacgccat cgacgacatg cgaatcctgt ccacgggcat
     1201 cgtttgcaac ggacggccca atcgcgcggg cttcatcatg ggcaatccgg ggcagccgct
     1261 gaccatcttc accgacaccg actgcaacgg cccgggcgga gcgcagggag acaacgacgg
     1321 ttgggtgacc gtggacctgg cgaacgacgg ctgggcttac ttctccggac cgaacggcgg
     1381 cggtacgtac acgggtacga tcaccggctt tccggagccg aagcgcacgt acaaggcact
     1441 cgaacttgtg ctcgatcgcg cctgggacca gaaatgggcg ctcaacgctt cctacacgct
     1501 ggcattcagc gagggcaacg ccgagggtcc ggtcaattcc gatttcaact tcggcgacag
     1561 tggccgtacc gaggccttcg acgatccctg ggtgcaggtg ggcggcgacg gatatctgcc
     1621 gaacgatcac cgtcatgcgt tcaagctgcg cggcacctac ggactcggcg agcactggag
     1681 cttcggcacc aacctctctg ccatttccgg ccggccgatc agcgcccagg gcgtgggcaa
     1741 cccgttcgac ggccattcgt tccattcctt ctacgtctgc accgcgaatt gcacgttgcc
     1801 ggccgcgcag cgggtctatg agttcaatcc gcgcggttca gaaggacgca cgccgtgggt
     1861 ctacgatgtg agcgcgagcg tcacgtggcg cgcttcactc ggcttcgccg acgcgcaagt
     1921 aagattggcc gtctacaacc tgctggacga tcacaatgcg cgcgaggccg aggaccgata
     1981 ccagaggacc atcggtggaa ccacgtgcgg taatgcttcg gaatgcggtg tgggtcagca
     2041 tcacgaattc ggactggcca cgacctatca gtcgccgcgt tacgcgcagt tgacgttcaa
     2101 gctggacttc tgatgtggca tcgagcaggc ggcctgccaa cgcgggccgc ctgctatcct
     2161 cgcgcagtga agacggtcag cctcggcggt tcgctgctcg cgctaacgag cgccattggt
     2221 ctttctgccc ccctcaccct cgacacacac gtcgacattc cgcgcactta catgcgcgag
     2281 gcgcgcttcg acgccggtac gaacaccgaa ctcaaggttg atctcggcaa aatgaagcgc
     2341 ggcggtctcg acgccgcatt cttcgtcatt ttcgtcgaac aggggccgcg cacgcccgaa
     2401 ggttacgccg cggcattcgc ggccgccgag cgaaaagtct cggccatcga agacatggtc
     2461 cgaaaatatc cggaccgcat tcgtctcgcc acgtcgtcca aggacgtcat cgacaatcac
     2521 gccgccgggc gcctctccgc gatgatgggc atcgagaacg gattcgtcat cggcaaggac
     2581 ctgggcaggc tcgatgcgct gtacgcgcgc ggcgcgcgct acatcggcct cacgcatacc
     2641 ggtcacaacg acatctgcac cagctctggt gctctgaaag agttcggcga tacgccggcc
     2701 tcggagaatg tgggactgag cgtattcggt gagagtgtgg tccgtcgtgc caacgcgttg
     2761 ggcatgatcg tggacgtctc gcacgcgtcg gatgcctgcg tgcgcgacgt gctgcgctgg
     2821 tcggcggcgc cgatcatcgc ctcgcactcc tccgcgcgcg cgctcacgaa tcacgcgaga
     2881 aacctgagcg atgatcttat gcgctcgatc gccgccaagg gcggcacggt gcagatcgtc
     2941 gcctacaccg gattcctgaa gctcgacccc gcgcgcgacg cggccgagaa ggcgctcgag
     3001 agcgagaccg cgcgccaggc gggcgatgcg gaattcgaca gcgcgaagca cgaatatctt
     3061 cccgcccatc agcaagggct cgcgcgtctc gacaaagacc atccactggc gacgctcgac
     3121 gattatctcg atcacatcca gcacgccgtt aaagtggccg gcatcgatca tgtcgggctc
     3181 tcctccgact tcgatggcgg cggcgcggtc cagggctgga aggatgcgag ccagacgcgc
     3241 aacgtcacgg cgggtctgcg caagcgcggc ttcactgagg cgcagatcgg ccagctgtgg
     3301 agcggcaatg tgttgcgcgt gtggcgcgac gtcgaacgcg tcgcgaaatc gtcaaccgtc
     3361 accgacaaga agactatcga tgccattttc gatgcggtgg tggcggagta ccacctgccc
     3421 ggcatggcgc tgggcgtggt cgaagacggg aaagtgacct acacgcgcac cgcgggcgaa
     3481 ctggtttccg gcggcggcaa ggcaatcgat gcagacacgc tgttcaagat cgcctccaac
     3541 acgaaggcaa tgaccaccgg cctgctcgcg cgcctggtgg atgccggcaa gctccagtgg
     3601 gacgaccccg tcgtcaaata cctgccccag ttcaagatga gcgagccgtg gatcacgcgc
     3661 gagatgcgcg tgcgcgacct gctcattcac aacagcggac tgcgcgaggg cgccggcgat
     3721 ctgatgctgt ggccggagcc taaccacttt acgcgtgcgg acatcatcgc ggggcttgcg
     3781 cacctcaagc cggtgcagag ttttcgttcg cactatgcct acgacaacct gctgtacatc
     3841 gtcgccggag aagtggcggc cgccgcaggt ggcgccacgt acgaagagtt ggtgcgtcgg
     3901 gaaattttcg ctgccactgg tctcaagcgt tgccaggtcg gcgaatggaa ccgcgacgcg
     3961 gtgggcaacg tggcacagcc tcacatgtgg gagggtgacc gcaacctcgc atatgcgcgt
     4021 gacccggaaa cggtgcccgc gatctcatcg gccgccgcgg gcggagttcg ctgcagcctg
     4081 aacgacatgc tcgcgtggat gcgcatgtgg ctggatcccg aactccggcc gcccgggcag
     4141 gagcgtccgt ggctcagccg cgcccagcac gacgcgttgt ggacgccgca gattccgatg
     4201 ccgatgtccg ggcggcagaa gcgctggaac aacggcaatt tttccgccta tggatacggc
     4261 tggcgattgt cggacgtcga tggcgtacgc cgcgtatcgc acaccggcac gttgggcggc
     4321 atgtattcgg cattcaccct gctccccgaa aagcgcagcg gcttcgtatt catgatcaac
     4381 ggcgagggct cgcgcgcgcg cacggtgctg aacgaagcgc tggtcaagca gttcacggcg
     4441 cccggccgcg cgccgtcagc cggctggtac atcgagcaac tgaagatgga agaccagtct
     4501 tcggcgtccg cttccggccc ggcgcacctg cagcttccaa tgcggcgtct tgcgtcgccg
     4561 tcttcgatga aaccctggct cgggcgttat cgcgatccct ggttcggtga gatctcgatc
     4621 tgcgaacgcg ccgggcacac gcggttcaac tcggtcaagt cacccctgat ggccggcgac
     4681 gtcatgcgcg tcggcgagcg cctgctggtc gactgggacg acgtgagcgg cgacgtagaa
     4741 ccctggctca acttcgcggc cggcaagcca gtcacgctga cgctcgccaa ggtcgatccc
     4801 gatggcgact tcagctcgga tttcgaagat ctgtccttca cgcgcatcgg cgcgtgcgtg
     4861 ccctcgccga aggtcgcccc attgagcgat gccgccgcca tcgcgaagat cgatgcactg
     4921 atgggcagct acgcgggcgc cgtgcccggc gcttccgtgc tcgtgctgcg cgacggcgcg
     4981 ccagtggtac gccgttcata cgggctggca gatctcgaac aacgcacgcc cgcctcgccc
     5041 gagactaact atcgcctcgc ctcggtgacg aagcagttca ccgccgccgc catcctgctg
     5101 ctcgccgagg cgggcaagct cgggctcgac gatcccgtgc gaaagtggct gccggagctc
     5161 cccgaggcgg cgcaacccgt gacgatccgt catctgctca cgcacaccgg cggtctcatc
     5221 gactacgaag atttcgtggc cgacgatgct ccgcaggtgc acgacgcgga tgtgctgcgt
     5281 ctgttggcaa agccagatcg cacctatttt ccggccggtt ccgcctatcg ctacagcaac
     5341 agcggttacg cactgctcgc gctggtcgtc gagaaggctt ccggccagcg gttcgccgca
     5401 ttcctgcacg accgcatctt cgcgccgctc ggcatgcacg ctaccgtcgc attcgaggac
     5461 ggtgtttcga ccgtcccgac gcgtgccttc ggccatagcg ccggcggcga ggcctggacg
     5521 cgcacggacc agagttccac cagcgccgtg ctcggcgatg ggggcatcta ttcatccgtt
     5581 gacgatctcg cgaagtggga cgcggcgctc tacgacaatc gactgttgcg cgaagaatcc
     5641 cggaagctgg cgttcacgcc ggccacgcat accgacaatc cgagcgtcga ctacgccttc
     5701 ggctggcgca tcaccggaga gacgctctgg cattcgggcg agacacgggg cttcagaaac
     5761 gtgatcatcc gttttccgga acggaaactg accgtcatca tgctcaccaa tcgcaacgac
     5821 cccgagcctt attccaccgc gctcgccatc gcgcagctct atctaccgtc ggctgggcat
     5881 ccgtgatcca caacgacgcg gtcatcctcg gtttactggc ggccacgctg ggcgtcgtct
     5941 tctggaccac gcaatccgcg cagccgttct ggaaacgctt ctacaagtat gtgccggcac
     6001 tcctgctttg ctacttcgtg ccggcgctct acaactcgtt cggtttgatc gacggcgagc
     6061 attcgaagct ctacttcgtc gcgtcgagat atctgctacc cgcgacgctg gttttgctga
     6121 cgctcgccat cgatttgcct gccacgttac ggttgggccc aaaagcgctg gcgatgtttt
     6181 tcgccggcac cttgagcgtg gtactcggcg gtccagtggc gctgctcatc tggaagtccc
     6241 tggcgcccgc cagtgtcgac ggcgagctgt ggcgcggcat gagcgccatc gccggcagct
     6301 ggattggcgg tgccgccaac caggccgcca tgaaagacgt gttcagcatt gagccgaatc
     6361 tcttcggcgt gatggtggcg gtcgatgtcg tgttcgccaa tctgtggtta gccgtgctgc
     6421 tgtggatggc ggcaaatcaa cagcgcgttg atcggtggct gaaggccgac accacctcaa
     6481 ttgccgcgct cggcgaaaag atggagcgtt tgcgccttga aaacgagcgc attcctttgc
     6541 ttcccgaact catgatcatg ctcgcgctcg ctttcggcgc taccggcctg tcccatgcgc
     6601 tggccgtacc gttggccagc gctttcgctg aagtcgagtg ggccgtgcgc ctgtcgctcg
     6661 ccagcgaatt cttctggatc gtcgtactcg cgacaacatt cgggctgctg ttgtccttta
     6721 cgcgcgccag aaatctcgaa ggcgctggtg cttccaggct tggctcggcc ttgctgtacg
     6781 tactcattgc gactgtgggc atgcagatgg acatcggcgc cgtgctcacg agccccgtgc
     6841 tgttcggtat cggtttcaca tggattgcgt ttcatgccgt tgtcattctg gtcgtcgcgc
     6901 ggctgatcag agcgcccgcg ttcttccttg ccgtcggcag ccaggcgaat atcggcgggg
     6961 ccgcgagcgc accggtggtc gcagccgcgt tttacccatc gctcgctccg gtcggggtgc
     7021 tgctggcggt gctgggttat gcgttgggaa cttacggcgg ctggctctgc gggcagctca
     7081 tgcggctcgt cagccagtag caattcaagg gctgcccacc ggcaacgccg atttccgcac
     7141 tatcatcata atgcccgtcc cggtgaattg aactgcccca ttcggtggag gacgcagtca
     7201 cgtccagcag gtagggtgcg cgaccatgaa tacaccctcc tcccccgtat ccgcgtcgtt
     7261 ttccggctac cagaaggtgg tggtcggcat gctggcgttc ctgcagttcg ccgtcattct
     7321 cgacttcatg ctgatggcgc cgctgggcgc cgtcatcatg ccggcgcttt cggtcacgcc
     7381 cgcgcagttc ggcacggtcg tctcggcata cgcgttcgcc gccggcattt cgggcctgct
     7441 cacggcagga ttcgccgatc gctttgacag aaaaagacta ctcctcttct tctatatcgg
     7501 cttcatcctc ggcacgttgt ggtgcggcct cgcgcaaagc ttcgaatctc tgctgctggc
     7561 gagaatcgtc acgggattgt tcggcggcgt catcggctcg gtcatcctcg ccatcgccac
     7621 cgacctgttt ccgccgcaga tgcgtggccg cgtcatgggt ttcatccaga ccgcattcgc
     7681 cgccagccag atcctcggca ttcccatcgg catctatctg tcgaaccaat ggaactggca
     7741 cgtgccgttc ctcgcactgg tggtgctcgg cgtcatcggc ggcttgctca tcgccgtcta
     7801 catgaagccg gtcgcggacc acctgaaggt gccgcaggaa cacagtgcct acaaccatct
     7861 gcgcaacacg gtgatcgagc cgcgctacct gatgtcgttc gtgtcggtgc tgttccttgc
     7921 gacgggtggc ttcatgctga tgccgttcag cagcgccttc acggtgaaca acctcgggat
     7981 ctcgctgcac gacttgccca tcatctacct ggtgacggga atcgccacga tcttcgtcgg
     8041 cccgctcgtg ggcaaagtcg ccgaccggtt cggcccgctg cgcgtgttct tcatgggaac
     8101 tgcgctgacc atcgtcatgg tgctgatcta cacgcacctg ggcgtctcga cgctggctgt
     8161 ggtcaccgta gtcaacgtcg tgctgttcat cggaatcttt tcgcgcatga ttccgttcca
     8221 gtcgctcatg tcccaggttc cagcgcaaac gcagcgcggg tcgttcaacg cgatcaacgc
     8281 gtccatctcg cagctcgccg gtggaatggc gtccttcgtc gccggacaca tcgtccagca
     8341 gggagcggac ggaaggctcc atcactatga tgtcgccggc tatgtcgtcg tcggaacgtc
     8401 actgctggcc tgcttcctca tgtggcgcat tcagcgcggg atctcagcga acgtggccgc
     8461 gctggcgcac tagccgcgtc atttgctggt tgcaggtcat gcgcggacag ggcatcgtgc
     8521 cggccatcag cgcccgctgg aagaagcgtc gcggtatctc gatgacgtcg aaaccgtaat
     8581 caggaagtgc gaggccgtca tgccatccca ccaggacttc gtcgcccgga acacctgcac
     8641 agcggggcag ccgtgaggcg cgcgccaatc cggctgctgg cgctcgccac gctcctgttt
     8701 gcactcgcgc cgtgtatgag catcgcggcc gacgtgcagg cctgggtgac gacggccgat
     8761 cggagccgcc tgctcgcgcg cgaatcgggg ctgtcgttcg ccacgcgggc cacgctcgcg
     8821 aatcgaatcg atgtcgacgc gggcaagcgc taccagccga tcgtcggctt cggcgcggcg
     8881 gtcacggatg cgagcgcatg gctcatccag acgcggctca gcgagacaca acgggccgcg
     8941 ctcatccagg agttgttcgg ccgcgatggt ggcctcggtt tgagtttcac gcgcatctcc
     9001 atcggcgcgt cggatttttc gcgcagtcac tacagcctcg gcgacacgcc cgacggcaag
     9061 ccggatccgg aactcgagca cttctccatc gagcccaacc gcgcggagct gttgccggtg
     9121 ctgcgcgcgg cgctggcgat caaccccgag cttcgcatcg tcggctcgcc ctggagtccg
     9181 ccaggctgga tgaagacctc gggcagcctg gttcacgggc gcctgcttcc cgaggctcaa
     9241 ccggcgctcg cggcctatct aattcgcttc gtcgacgcct tcgctgcgga aggcgtgcct
     9301 atattcgcgc tgacgcttca gaatgaaccc gacttcgatc cgccggacta tcccggcatg
     9361 aagctggaac cggccgagcg agcacgcatc atcggtcagt ttctcggccc catgcttgca
     9421 aagcggacgg caccaccact catcctcgac tgggatcaca actgggacaa gccggagtca
     9481 cctctcgccg tgctcgccga ccgcgaggcc gcgcgttaca tcgccggcgt cgcctggcat
     9541 tgctacggcg gtaacgtcgc ggctcaaacg caggtgcacg acgcgcatcc ggacaaggac
     9601 acgtatttca ccgaatgctc cggcggtgaa tgggatccgg ttttcggcaa ttcgctgcgc
     9661 tggatggttc gcgagctcat catcggttcg acgcgcggct ggtcgcgcgg catcgtgctg
     9721 tggaatctcg cgctcgacga gaaccacggg ccgcacgcgg gcggctgcgg agattgccgc
     9781 ggtgtcgtga ccatcaactc gacgactggc gcgatcacgc gcaacgtcga gtactacgcg
     9841 ctcgcgcacg caagccgctt cgtcatgccg ggcgcgcatc gcatcgagtc gacgaccggc
     9901 atcaagggtc tggcgaccgt ggcatttcag aacgcggacg ggtcgatggc gttgatcgtc
     9961 ctgaacgacg cgaaacgccg gcgcaagttt tccgtccgct ccgccggcgc gtcattcgac
    10021 tacgcgctac cagccggcgc cgttgcgacc ttcgtctgga atccgaagag ttagcgggga
    10081 gcgcccaaca cctcgcgcag cgggggcgcg gcattcggat tgaaagcttg aaagtcttct
    10141 cccacgagcg ccgcgatggt cgacgcgacc tgcgattgag tgactgccac tgaagcctgg
    10201 cgctcgccga ggggctgtgt gtccggcccg atgaccgcga tccagattgc atcggatccc
    10261 ttttgatcga cgccgtggtc acgccactgg gtggggccgc tgccgcggcc atggtccgtc
    10321 gacacgacga gcgttgtttt atccttgtag tgcgccgaag actgcagctg ccgccacagg
    10381 tccgcgagat aggcatcgaa cgaatgcgcg gtttcgagga acgcatcgta gcgccccatg
    10441 tgctgccagg tgtccgtgtc gccgtacccg atgaacatca cgcgaggttg atgggcgcgc
    10501 aggtgttcgc ggaccacgac gtgcaggaac gaatcgtagg gatcgtcgcc ttcgatccgg
    10561 gtggtggtgc gatagagttc gctcagcaac aggccatgcg cgctgcgatc attcgcatcg
    10621 accagggtcg cgccagcgcg aatcggaagt ccgctgcgcg aaccgttgaa gatgtccgcg
    10681 aacgtcgccc aggttccgaa gatctcgacc ttgcccgcaa gacccggccg tgtgttgagc
    10741 cactcgaaga ccgtcacgtt cgggttcggc ccgaattcat tcgtatcgat gcgcggatcg
    10801 gctgctccgc tcgccatctc gttgtatccg gggtacgaga accacctcgt gttcgtcacc
    10861 cgcgccacgc tgcccttgtg ctggttgccg tagatctggc cttgggtcgc gaccgtttcc
    10921 cacaggaacg gaaacaactt gcggcgccgc accgaaggat cttcgtccca gtattttttc
    10981 ctgagtttgt cgttcaacag cgtgggatcc gcacccgtga acacttcctg ccagcgcacg
    11041 ccatcggcga tgacgagaat cacgttgcgg gtgtcgccgg cccatgcagc cgaggcgaag
    11101 cacaacgcta caccggcaat cgcgcgaaac agcagcttgg tattcatgag gggaagatac
    11161 tcctgacgaa gggtttcacg catttcgcct cgatactgca gacactcctg ctggctgcgc
    11221 cgctggccgc ggcgcccgcg acctgtcaca tcgatccgga acactattca tcagcaacgg
    11281 cgtgagaccg ttccgtcagc caaacaactt cttcgcaatc gttgcttggt gaaggcagac
    11341 cgtgcgggcg tatcatgcgt gcacgcgtcc gaggcaaggc tcatgtcacc attcgaccga
    11401 cgttcattcc tgtcgctggc cggcagcgcc gcggccggct cgctcttgtc ccgatccgcg
    11461 caggcggcgg ccacgcggcc caacatcctc ttcatcctcg ccgacgatct cggctacgcc
    11521 gatctctcgt gttatggccg cggcgacctc cgcacgccgc ggctcgatcg catggcccgc
    11581 gagggggtgc gtttcctgca gtcgtactcc agctcgcccg tgtgttcggc cacgcgtgcc
    11641 gcgctgatga cgggccgcta tcaataccgc ctgcgcgtgg gactggagga gccgctgcgc
    11701 gacaccgcgg agatcgggct gccgccggag cagcccacgc tgccctcgct gttgcgcgac
    11761 gccggatatc gcacctcgct ggtgggcaag tggcacctcg ggctgttgcc gaaattcggc
    11821 cccttgcaga gcggctacga atctttctac ggagtgcgcg gcggcggcgt cgactactac
    11881 agccatgtcg gcaccggcgg ccgcaaggat ctctgggatg gcgaccagcc gctggacacg
    11941 cagggctact tcacagaact gctcaccgag cgggcgctgc agcaactgcg cacgttcact
    12001 cgggaacaca cgccgttctt cttgagcgtg cactacacgg cgccccactg gccctgggaa
    12061 cctcccggcg acgacagcga gtcgcgtcga atcgccggca ggagcctgtt ccattacgac
    12121 ggcggcaccc atagcgtgta ccggcagatg gtcgagcacc tggatgcgca ggtcggacgg
    12181 ctgctcgacg cgctggcgca gtccggcgcg gcgcgcgaca cactcgtgat attcaccagc
    12241 gacaacggcg gtgaacgctt ctcgaacacc tggcccttca gtggacgcaa gggagaactg
    12301 ctcgaaggcg gcgtgcgcgt gcccacgctg ctgcgctggc cgcgcgccat ccgcgccggc
    12361 gcgaccagcg aacaggtcaa cgtcaccatg gactggctac ctaccttgct cgcggccgcg
    12421 ggggttgcgc ctgatcccgg cgctccgtcc gacggcatca acctgttgcc gtccctcgcg
    12481 cctggtgcga accccgtccc ccgcaaattg ttctggcggt tcaaggcgaa ccatcagcgc
    12541 tcggtacgcg acggtgactt caagttgctc aagatccaga acaacgtgtt cctgttcaac
    12601 gtcgtggagg acccactgga gcgggccaac ctgcgcgacc ggcaccgcga ggtcttcgag
    12661 cgactcaagt cggagtggga ccagtggaac gccgccatgc ttccccagct gccggagacg
    12721 ctcgcctcgc cgccgtcggg cagcgacgcg cccgatcgtt acggaacatc gccaccgccc
    12781 aactcggtgg atgatgtctc gacgtggccc ggcgccttgc ccgcgaagct ctgaggcacc
    12841 ggcccaccaa tctggagagc ttgggagctc aggttgaggc gcaatggcgc gcgatgaatt
    12901 cggcgtgcgt gggcattgcc tcgacacatg ccgcgatgac gcgctgcgtg tcggccagga
    12961 aggcgcgtac ctcgtcgagc gggcgcaagt cggcaaatgg gtggtagccg ctcgcgcgaa
    13021 tacgctgccc ctccatcacc tgcacccagc tcaactcgcc aaacagctca ttgtcttcac
    13081 ggaagaagcg tccgttgctc cgatacaggt cgatcttgtg ctgcagcgtc ggcggcactt
    13141 ccatcgtgcg gcagtactgc cagaacggag tgtcgtcgcg ttccgtggcc ttgtagtgca
    13201 ggacgatgaa gtcacgcgcg cgctcgtact cgatgcgcgt ctgcgtgttg agctcgtcga
    13261 tctcggcctg atcgaagccg gcccgcggaa acagcgacat cagccgcgag atcgccgtct
    13321 ggatcaggtg gatgttggtg gactcgatag gttcgaagaa cccggccgcc agacccaagg
    13381 cgatgcagtt gcggttccat acgcgcttgc gccggcccgg catgaagggg atgtgccgcg
    13441 gttcggccag cggctcgctg tccaggtttc ccgtcaggat cgcagtggca tcctcctggc
    13501 tcatgaagcg actggagtag acgtggccat tgccgatgcg atgctgcagc ggaatgcgcc
    13561 actgccagcc ggcgccatgc gccgtggcgc gcgtcatcgg gatcaggggc tccacggagc
    13621 ggctggggac cgcgaccgca cggtcacagg gcaaccagtg cgaccaatct tcgtagccgg
    13681 tatgtaaggt cttctcgatg agcaggccct gcatccccga gcagtcgacg aaaaaatcac
    13741 cggcgacgcg actaccgtcg tccaggacaa ctgcattgat aaatccgtct ggctcgcgct
    13801 gctgcacgga gacgatcttg ccttcaatgc gttcgacgcc gtttcgctcg gccagccggc
    13861 gcaggaatcg ggcatacagt ccggcatcga agtggtacgc gtaggtgatt tcacgcagcg
    13921 gcgagcccgc catgtccggc cgtggccgca tgaacctggc gcgtcgcggg gccgcggtgt
    13981 tgatggagta gtcgccgagg ctggaagcca agccagcctg cgcaccgcgc aaccagaaat
    14041 gatggaacgg cagtccatcc aggtcgggcc cgagcttgcc gaaaccgtgg gtgtagcggt
    14101 cgccgatcgc gctccagttc acgaactcga taccgagctt gaacgtgccg cgcgtggcgc
    14161 gcaggaactc gtcttcgtcg atgccgagaa tgcgattgaa ggagacgatg tgcgggatcg
    14221 tcgcctcgcc gacaccgatg atgccgatct cgtcggattc cacgagccgg atcgaacacg
    14281 cgcgtccgaa gtggttcgaa agcgcggcgg ccgtcatcca acccgcagtg ccaccgccta
    14341 cgatgacgat gtcacggatc agcggacccg tcatgcctcg actccgcgcg acgacacctc
    14401 agaacttgta cgacgcgccc aacatgatca cacgtccata gttgtcgtct t
//
LOCUS       DASQJS010000023         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4095847, whole genome shotgun sequence.
ACCESSION   DASQJS010000023 DASQJS010000000
VERSION     DASQJS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4095847"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VEW08_01105"
     CDS             complement(<1..356)
                     /locus_tag="VEW08_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HYJ39351.1"
                     /translation="MDNKLQLEESSGYDRRVIDYIQRASASGLYEIRGLGAKRHLPSF
                     DDLVFLGASLSRYPLEGYREKCSTRTVLGTRHATKPIELATPITIAGMSFGALSARVK
                     EALGRAATAVGTSTTTG"
     gene            complement(358..1050)
                     /locus_tag="VEW08_01110"
     CDS             complement(358..1050)
                     /locus_tag="VEW08_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39352.1"
                     /translation="MESVKLFDLATTPLREVNRFLHRDAIDAGVKRVKILNADGAHSI
                     AAGLNAPLDVSIDGHAGYYAGGMNMHANITVHGNAGPGVGENMMSGSIRVEGFASTAA
                     GASAHGGLLVILGDAALRCGISLKGGDIVVGGDVGSFSAFMAQAGRIVICGDAGDALG
                     DSLYEAVIYVRGRVRSLGADARFEPMTDADYVAVRELLARAGLAHDPESFQRVASAKG
                     LYHWHADANQEY"
     gene            complement(1038..1931)
                     /locus_tag="VEW08_01115"
     CDS             complement(1038..1931)
                     /locus_tag="VEW08_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HYJ39353.1"
                     /translation="MCGIVGLLLKKPELRDQLGELMTPMLVGMTSRGPDSAGVAIFGS
                     ANADFHKLSLFWGEGAADWKRLAAELTAEFDGKHRFRETGQHAVLTTRADPEAVKAWI
                     GEAAPEIYVLSMGRSIDLYKDIGAPADIVERYALNKVTGTHVVAHTRMATESAVTPAH
                     AHPFTAGQDFCLVHNGSLSNPHLVRRKLEPLGIRFVTDNDTEAACRFFEWRMREGDDL
                     TTAVHRGFSELDGFFTFLMGTDKEMLIVRDAFACKPAVVAETDDYVAIASEFRSLAHL
                     PGVRHANVFEPLPEEVYSWKA"
     gene            complement(1931..>2933)
                     /gene="glnT"
                     /locus_tag="VEW08_01120"
     CDS             complement(1931..>2933)
                     /gene="glnT"
                     /locus_tag="VEW08_01120"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HYJ39354.1"
                     /translation="RLAERGITMYVGIEPEFMLLGRDPAGKLGPFDPTDDLDKPCYDY
                     KGLSRASGVLDEITLSLRSVGIDVYQIDHEDANGQFEVNFTYADALKSADNFTFVKMA
                     ASEIARKHGMVATFMPKPFSNRTGSGAHFHISLGNDQQKNLFEDKSDKRGLSLSTMAY
                     QFLGGVLAHARALAAVCAPTVNSYKRLVVGRALSGATWAPAYVAYGDNNRTACVRIPG
                     GRLELRLPDSGCNPYLALAAVVAAGIDGIDRQLDPGEPTNLNMYELSEAQLQERKISL
                     LPQNLLEAIGALETDAVVKAGLGAELAAEFIKLKRMEWTEYARHVSDWETRRYLEFF"
BASE COUNT          511 a          957 c          943 g          522 t
ORIGIN      
        1 cccgtggtcg tcgaggtacc caccgccgtc gcagcgcggc cgagcgcctc tttcacgcgc
       61 gccgatagcg cgccgaagct catgcccgcg atggtgatgg gggtggcgag ctcgatgggc
      121 ttcgtggcgt ggcgcgttcc gagcacggtt cgtgtcgaac atttctcgcg ataaccctcg
      181 agcggataac gcgacagcga cgcgcccagg aagacgagat cgtcgaacga cggcaggtgg
      241 cgcttggcac cgagcccgcg tatttcatac agacctgagg ccgacgcgcg ctggatgtag
      301 tcgatgacgc gccggtcgta gccggacgac tcttcgagct gcaacttgtt atccatttca
      361 atattcctgg ttggcgtcgg catgccagtg gtagaggccc ttggccgacg ccacgcgctg
      421 gaaggattct ggatcgtgcg cgagtcccgc gcgcgctaac aactcgcgaa ccgccacgta
      481 gtcggcgtcg gtcatgggct cgaagcgcgc atcggcaccg agcgagcgca cccggccgcg
      541 aacgtagatc accgcctcgt acagtgaatc accgagcgca tcgccggcat cgccgcagat
      601 cacgatgcgc cctgcctggg ccatgaacgc cgagaagctg cccacgtcgc caccaacaac
      661 aatatcgccg cccttgagcg aaatgccgca gcggagcgcg gcgtctccga gaatcaccag
      721 aagtccgcca tgcgccgatg ctccggctgc cgtggacgcg aaaccttcga cgcggatgct
      781 ccccgacatc atgttctcgc ctacgccggg gccagcgttg ccatgcaccg tgatgttggc
      841 gtgcatgttc atgccgccgg cgtagtagcc ggcgtggccg tcgatggaca cgtccagcgg
      901 tgcattgagc ccggcggcga tggaatgtgc gccgtcggcg ttgagtatct tcacgcgctt
      961 cacacctgcg tcgatcgcgt cgcggtgcag gaagcgattc acctcgcgca gcggtgtcgt
     1021 ggcgaggtcg aagagtttca cgctttccac gagtacacct cttcgggtaa cggctcgaat
     1081 acattcgcgt gccgcacgcc cggcaggtgc gcgagcgaac ggaactccga ggcgatggcg
     1141 acatagtcgt ccgtctcggc aaccaccgcg ggcttgcagg cgaaggcatc gcgcacgatc
     1201 agcatttcct tgtcggttcc catgagaaac gtgaagaagc cgtcgagctc ggaaaagccg
     1261 cgatgcacgg cggtagtcag atcgtccccc tcacgcatgc gccactcgaa gaaccggcag
     1321 gcggcctcgg tgtcgttgtc ggtgacgaag cggatgccga gcggctcgag tttcctgcgc
     1381 accaggtgcg gattggacag cgagccgttg tgcacgaggc agaaatcctg gccggccgtg
     1441 aacgggtgcg catgcgcggg cgtgacggcg gattccgtgg ccatgcgcgt gtgcgccacc
     1501 acgtgcgtgc cagtcacttt gttcaaggcg tagcgctcga cgatgtccgc cggcgcaccg
     1561 atgtccttgt acagatcgat ggagcggccc atcgacaaca cgtagatttc gggcgcggct
     1621 tcgccgatcc aggccttgac cgcttcgggg tcggcgcgcg tcgtcagcac ggcgtgctgg
     1681 ccggtctccc tgaaccggtg tttgccatcg aactcggcgg tgagctccgc cgcgaggcgc
     1741 ttccagtccg cggccccctc gccccagaac aacgacagct tgtgaaaatc agcgttggcg
     1801 ctgccgaaga tcgcgacgcc tgccgagtcc ggaccacggc tggtcatgcc caccagcatg
     1861 ggcgtcatga gctcgcctaa ctgatcgcgc agctccggct tcttgagcag cagcccgacg
     1921 atgccacaca tcagaagaac tccaggtatc gcctggtctc ccagtcggag acgtggcgcg
     1981 cgtattcggt ccattccatg cgcttgagct tgatgaactc ggccgcgagc tccgcaccca
     2041 ggcccgcctt cactactgcg tcagtttcga gcgcgccaat ggcctccagc aggttctgcg
     2101 gcaagagcga aatcttcctt tcctggagtt gcgcttctga caactcgtac atgttcagat
     2161 tggtgggctc gcccggatcg agctggcggt cgataccatc gatacccgcc gccaccactg
     2221 ccgccagcgc gagataaggg ttgcagcccg agtccggcaa ccgcagctcg agccgcccgc
     2281 ccggaatgcg cacgcaggcc gtgcgattgt tgtcgccgta ggcgacatac gccggcgccc
     2341 aggtggcgcc cgacagcgcg cgccccacga cgagtcgttt gtacgagttc accgtgggag
     2401 cgcacaccgc cgccagcgca cgagcgtgcg ccagcactcc ccccagaaat tgataggcca
     2461 tggtcgagag cgacaatccg cgcttgtcgc tcttgtcctc gaagagattc ttctgctggt
     2521 cgttgccgag cgagatgtgg aagtgcgcgc cactgcccgt gcgattcgag aagggcttgg
     2581 gcatgaacgt ggccaccatg ccgtgtttgc gcgcgatctc gctggccgcc atcttcacga
     2641 acgtgaagtt gtcggcggac ttgagcgcat ccgcgtacgt gaagttcacc tcgaactggc
     2701 cgttcgcatc ctcgtggtcg atctggtaga cgtcgatgcc cacgctgcgc agggaaagcg
     2761 tgatctcatc gagcacgccg ctggcgcgcg acaggccctt gtagtcgtaa cagggcttgt
     2821 cgaggtcgtc ggtggggtcg aaggggccga gcttcccggc cggatcccgg ccgagcaaca
     2881 tgaactcggg ctctatgccg acgtacatcg tgatgccgcg ctcggcgagc cgc
//
LOCUS       DASQJS010000024         5042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1899855, whole genome shotgun sequence.
ACCESSION   DASQJS010000024 DASQJS010000000
VERSION     DASQJS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899855"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="VEW08_01125"
     CDS             <1..1680
                     /locus_tag="VEW08_01125"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HYJ39355.1"
                     /translation="AKISAAIAGKDEAAANDVRKSTLTQLEGECEAQTKQKCQSVTLY
                     EGGQYWLYKYKRYTDVRLVFAPEASIAAFGGDPDNFQFPRWCLDMGLLRAYEDGKPAK
                     PANFLKINFAGPAAGEAVFVSGHPGATDRGLTLAQLKQQRNLDLPQWLLRASEIRGRL
                     IQFSASSPQNERITADLLNGLENGIKVRRKMLDALHEDSLLARKAAEQATLEKNVAKN
                     VALKKSTGNPWGDIDVALAAERGIFQPLNYIENGAGFQGRLVNYARTLVRGTAEREKP
                     NGQRYREFTDSALPRVQQNLGASVPVYPELEELRLGASLERMREWLGPDHPVVRKLMS
                     KESPAALAKRLVTETKLADPAVRLELWKGGSAAIAASNDPMIVLARSIDADARAVRKS
                     YEDTVEAPLRSATERIARARFSTLGTSVYPDATFTLRLNYGTVQGWNENGKPVEPFTR
                     LSRAFERATGSDPFRIPESWERVKGKLDMNTPFNLSTNNDIVGGNSGSPLINARGEIV
                     GLLFDGNIHSISGAYWFDTEKNRAIAVHPAIMREALSKVYAADAVLAELAR"
     gene            complement(1705..2448)
                     /locus_tag="VEW08_01130"
     CDS             complement(1705..2448)
                     /locus_tag="VEW08_01130"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYJ39356.1"
                     /translation="MSTRKACHGFPLRVVVAGLVLWGLAACQTSPPVAETPSTVPAPP
                     TPSAPPAGAREYAVVAEESLLQILVFRGGTMARLGHNHVIASHQLSGSVFVTDDPLQS
                     RFDISVPVNELTIDEPAMREMAGPDFPPGVPQSARDGTRKNLLSEALLDGEKYPTIQL
                     RTTDVVAAAEGFDVGVEIRIKDQVRNVRVPITLENKEGALVARGEFPLRQSELGLKPF
                     SVAMGSLVVLDEMRIRFEVVARMGAAPFP"
     gene            2511..3062
                     /locus_tag="VEW08_01135"
     CDS             2511..3062
                     /locus_tag="VEW08_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HYJ39357.1"
                     /translation="MTFRNTNQSWGSLTKALHWIIVILIINQWVIAERAEELKGLAKL
                     QTLGWHKSFGMTILMLAIIRLVWRWMNPVPDLTAETRPWERVLARISHVLLYALIFAM
                     PLTGWMMSSARNFPVSWFKLFQFPDLVAPSDQLFHRMQDLHHLLFSVLVTVALLHIAG
                     ALKHHFIDRNDVLKRMLPFGGVK"
     gene            3067..3708
                     /locus_tag="VEW08_01140"
     CDS             3067..3708
                     /locus_tag="VEW08_01140"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYJ39358.1"
                     /translation="MTNYIVSMAAAVALLTGACHAADTPATPQPAAKPAAAAPAATTT
                     PAAAVQRWVQAPGSSLTFTFDQEGAASKGSFRQFSTELTYDDKSPAAGSLNVKIQIAS
                     IDTQDQERNDMLASADLFDTAKFPTAQYSSSSFARTAAGGLEAVGKLTLRGVTHDLRL
                     PLKIVPTAAGLELSGATAIRRLDYGVGQGDWKSTEGVADEVKIQYKVALVKAK"
     gene            3719..4246
                     /locus_tag="VEW08_01145"
     CDS             3719..4246
                     /locus_tag="VEW08_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39359.1"
                     /translation="MTNSMRHVWMGISLSVALLTAYPAWTGSTSWWMTGIALFVFAIF
                     LLEPWFLTWELRRRGEALQITDEGVLRRLARGDSEFVRWSELREVSIVLTQGVNLSED
                     YFYVLAGAGSSGVLVGQVLATKYDLLSHLSKLPGFDHRNITTAMASPVNQKITLWRAA
                     PIEGEVEVLPSRALN"
     gene            complement(4268..4750)
                     /locus_tag="VEW08_01150"
     CDS             complement(4268..4750)
                     /locus_tag="VEW08_01150"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ39360.1"
                     /translation="MTLKKPALDPMTLAPRTSSGYPEPYRSRVLPREKRALGDPLGLT
                     KIGINYTTLPPGKESSMRHWHTHEDEFIFVLSGEIVVKTDAGEQTLGAGMCAGFPASA
                     DGRTGDGHQLINRSSEPAVYLEISNRDKADRAFYPDVDLHFHGTGAAVMFTRKDGSRF
                     "
     gene            4791..>5042
                     /locus_tag="VEW08_01155"
     CDS             4791..>5042
                     /locus_tag="VEW08_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39361.1"
                     /translation="MPQMFDDVGEIVEEALRRVGKRVVLALPLGIGKPNLIANEFFRR
                     ARGDATLDLTIFTALSLRKPTGSSPLEKAFIDPLAARVFG"
BASE COUNT          948 a         1628 c         1565 g          901 t
ORIGIN      
        1 gcgaagatat ccgcagccat cgccggcaaa gacgaagcgg ccgccaacga cgtgcgcaaa
       61 tccacactga cgcagctcga aggtgaatgc gaggcgcaga cgaagcagaa atgccagagc
      121 gtcacgctgt atgagggcgg ccaatactgg ttgtacaagt acaagcgcta caccgacgtg
      181 cggctggtgt tcgcgcccga ggcgtccatc gcggcctttg gcggtgaccc cgacaatttc
      241 cagtttccgc gctggtgcct cgacatgggc ctgctgcgcg cctacgaaga cggcaagccg
      301 gccaagccgg ccaacttcct caagatcaac ttcgccggcc ctgcggccgg tgaagcggtg
      361 ttcgtgtccg gccatcctgg cgccactgat cgtgggttga cgctggcgca gctcaagcag
      421 cagcgcaatc tcgacctgcc gcagtggtta ctgcgggcgt ccgagattcg tggccgcctg
      481 atccagttct cggcatcgag cccgcagaac gagcgcatca ccgccgatct gctcaacggc
      541 ctcgaaaacg gcatcaaggt gcgtcgcaag atgctcgacg cgctgcatga ggattcgctg
      601 ctggcgcgca aggccgccga acaggcaacg ctcgagaaga acgtggcgaa gaatgtcgcg
      661 ctgaagaaga gcaccggcaa cccctggggc gatatcgacg tggcgctggc cgccgagcgc
      721 ggcatcttcc agcctttgaa ttacatcgag aacggcgcgg gcttccaggg ccgactcgtg
      781 aattacgcgc gcacgctggt gcgcggcacg gccgagcgtg aaaagcccaa cggccagcgc
      841 taccgtgaat tcaccgacag cgcgctgccg cgcgttcagc agaatctcgg cgcctcggtg
      901 cccgtgtacc ctgagctcga agagctgcgc ctcggcgcca gtctcgagcg catgcgcgag
      961 tggttaggcc cggaccaccc cgtggtgcgc aagctgatgt cgaaggaatc gcccgcggcc
     1021 ctcgccaagc gcctcgtgac cgagacgaag ctggcagacc cggccgtgcg gctggagctg
     1081 tggaaaggcg gttcggccgc catcgccgcc tcgaacgatc ccatgatcgt gctcgcgcgc
     1141 agcatcgatg ccgacgcgcg cgccgtacgc aaatcctacg aagacaccgt ggaagcgccg
     1201 ctgcgctccg cgaccgaacg cattgcccgc gcacgcttct cgacattggg caccagcgtc
     1261 tatccggacg ccacgttcac gcttcgcctg aactacggca ccgtgcaggg gtggaacgag
     1321 aatggcaagc cggtcgagcc gttcacgagg ctgtcgcgcg ccttcgagcg cgccacgggc
     1381 tcggatcctt tccgcattcc cgagagctgg gagcgcgtga agggcaagct cgacatgaac
     1441 acgccgttca acctgtccac caacaacgac atcgtcggcg gcaattcggg cagcccgttg
     1501 atcaatgcca gaggcgagat cgttggcctg ctgttcgacg gcaacatcca ttcgatctcc
     1561 ggcgcgtact ggttcgacac cgagaagaac cgcgccatcg ccgtacatcc cgccatcatg
     1621 cgggaggcgc tgtccaaggt ttatgcggcg gatgcggtgc tggctgagct agccaggtaa
     1681 tagatagggg gcagctccct tttgctaagg aaagggtgct gctcccattc tcgcgacgac
     1741 ttcgaagcgg atgcgcattt cgtcgagcac caccagcgac cccatggcga cgctgaacgg
     1801 cttgagcccc agctccgatt gcctgagcgg aaattctcca cgcgcgacca gcgcgccttc
     1861 cttgttttcc agcgtgatcg ggacgcgtac gttgcgcacc tgatccttga tcctgatctc
     1921 cacgccgaca tcgaatcctt ccgccgcggc gacgacgtcg gtggtgcgca actggatggt
     1981 cgggtacttc tcgccatcca acaacgcttc cgacagcagg ttcttgcgcg ttccatcacg
     2041 cgcgctctgc ggtacccccg gcgggaagtc cggaccggcc atctcgcgca tggccggctc
     2101 gtcgatggtc agttcgttca ctggaacact gatgtcgaaa cgcgactgca gcggatcgtc
     2161 agtgacgaac accgaacccg atagttgatg cgacgcgatg acgtggttgt gaccaaggcg
     2221 agccatcgtg cccccgcgaa agacgaggat ctgcagcaac gactcttcgg cgacgacggc
     2281 atattcacgc gcgccggcgg gtggcgccga tggcgtgggc ggagccggta cggtggacgg
     2341 agtttcggcg actggggggc ttgtctgaca cgcggccagc ccccatagca cgaggcccgc
     2401 aacaaccact cgaagcggga acccgtgaca ggcctttcgt gtactcatga cgtgtaactc
     2461 atccttccgt ggctttgacc ggactcatcc ccagcggcta gcttaccggc atgacttttc
     2521 gtaacaccaa ccagtcatgg ggttccctga ccaaggcgct gcactggatc atcgtgatcc
     2581 tcatcatcaa ccagtgggtg atcgcagaac gcgccgagga actcaagggg ctcgcaaaac
     2641 tgcagacgct gggttggcac aagtcattcg gcatgaccat cctgatgctg gcgatcatcc
     2701 ggttggtttg gagatggatg aatccggtgc cggacctcac cgccgagacc aggccatggg
     2761 agcgcgtact cgccaggatt tcccacgtgt tgctctacgc actgatcttc gccatgccgc
     2821 tcacgggctg gatgatgtcc tcggcgcgca attttcccgt gagctggttc aagctgttcc
     2881 agttcccgga cctggtggcg ccgtccgatc agctttttca ccggatgcag gatctgcatc
     2941 acctgctgtt cagcgtcctg gtgaccgtcg cgctgctgca catcgccggc gcgctcaaac
     3001 atcacttcat cgacaggaac gacgtactga agcgcatgtt gcccttcggc ggcgtcaagt
     3061 gaattcatga ctaactacat tgtttcgatg gcagccgcgg tcgcgctgct caccggtgcc
     3121 tgccacgccg ccgacacccc ggcaacgccc cagcccgccg caaaaccggc cgccgcggca
     3181 cccgccgcca ctacgactcc cgccgccgcg gtccagcgct gggtgcaggc cccgggcagc
     3241 agtctcacat tcaccttcga ccaggaggga gccgcgagca agggatcgtt caggcagttc
     3301 agcaccgaac tcacctacga tgacaaatct cccgcggcgg gcagcctcaa cgtgaaaatc
     3361 cagatcgcct cgatcgacac ccaggaccag gagcgcaacg acatgctggc gagcgccgat
     3421 ctgttcgaca cggcaaaatt tccgaccgcg cagtacagct cgagcagctt tgcgcgcacg
     3481 gccgccggtg gcctcgaggc cgtgggcaag ctcacattgc gcggcgtgac acacgacctt
     3541 cggctgccac tgaaaatcgt accgactgct gccggcctcg aactctccgg cgccacggcc
     3601 atcaggcgcc tcgactacgg cgtcggccag ggagactgga aatccaccga aggcgtggcg
     3661 gacgaagtga agattcaata caaggtcgcg ctggtcaagg cgaagtaatg cgatgacgat
     3721 gacgaactcc atgcgccacg tctggatggg catttcactg agcgtggcgc tgctcacggc
     3781 ctatccggcc tggaccggca gcaccagctg gtggatgact ggcatcgcgc ttttcgtgtt
     3841 tgcgatattc ctgctcgaac cctggttcct cacctgggag ctgcgcaggc gcggcgaggc
     3901 actgcagatc accgacgaag gcgtgttgcg ccggctggcc cgtggcgaca gcgagttcgt
     3961 gcggtggagc gagctgcgtg aagtgtccat cgtcctgacg cagggcgtga acctgagcga
     4021 agactacttt tacgtgcttg ccggcgccgg ctcttcgggg gtgctggtgg gccaggtgct
     4081 cgccacgaaa tacgacctgc tttcgcacct gtccaaacta cccggcttcg accaccgcaa
     4141 catcaccacg gcgatggcat cgccagtgaa ccagaagatc accttgtggc gcgcggcccc
     4201 gatcgagggc gaggtcgagg tgctgccgag tcgtgcgctg aactgatcgg cctcggcgat
     4261 acgccgttca gaacctgctg ccatccttgc gcgtgaacat cacggcggcc ccagtcccat
     4321 ggaagtgcag gtcgacatcg gggtagaagg cccggtccgc cttgtcgcgg ttgctgatct
     4381 cgagatatac cgctggctcg ctgctgcgat tgatcagctg atgtccgtca ccggtcctgc
     4441 catcggcgga agcaggaaat cccgcgcaca tcccggcgcc cagcgtctgc tcgccggcat
     4501 cggtcttcac cacgatctcg cctgacaaca cgaagatgaa ttcgtcctcg tgtgtgtgcc
     4561 agtggcgcat cgaagattct tttcccggcg gcagtgtcgt gtagttgatg ccgatcttcg
     4621 tcaatcccag gggatcgccg agcgcgcgct tttcgcgtgg cagaacgcgc gaacgatagg
     4681 gctcgggata tcccgaactg gtgcgcggcg cgagcgtcat gggatcgagc gcgggctttt
     4741 tcaaagtcat atgcgttgac ccttttccga ctgcgtgcaa gtgtagcgcc atgccgcaga
     4801 tgtttgatga tgtcggtgag atcgtggaag aggcgctgcg gcgcgtgggc aagcgcgtgg
     4861 tgctggcact tccgctcggc atcggcaagc ccaacctcat agccaacgaa tttttccggc
     4921 gcgcacgcgg tgacgccacg ctggacctga ccattttcac ggcgttgagc ctgcgcaagc
     4981 ccacgggatc gagcccgctg gagaaagcgt tcatcgatcc gctggcggcg cgggtgttcg
     5041 gc
//
LOCUS       DASQJS010000025         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4097053, whole genome shotgun sequence.
ACCESSION   DASQJS010000025 DASQJS010000000
VERSION     DASQJS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4097053"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="VEW08_01160"
     CDS             complement(<1..1607)
                     /locus_tag="VEW08_01160"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HYJ39362.1"
                     /translation="MEVKNTRRDFVIGSGAVLAVSNLPAGSAIPSGDKAAETLLAAVT
                     EELLVDYPESATQLGIDNGARAALKSKLGDRSAAGQKAIAKRVAQRIDRLKSIDTSRL
                     GNAARIDVDVVRTAHEFALEGFAFPYGDVALLNSQWSWRNAPYVVAQNTGAFLEIPSM
                     LDEQHTVGNAADADAYLARMEAYAGQLEGETGRLKSAAAQSVVAPDFLLDKTLKQIKL
                     ARSGNVADWSLVASLAKRTKNMPGNYAARAAKLATDKVAPALDRQIAELEVHRKQANN
                     DAGVWKLPRGDEYYAWTLRAGTTTRMTPDEIHARGQEELRALQSEMDGILKTQGMSQG
                     TVGERMTALGKQDRFRFEESDAGRAKLMAFLNARVADIRTRLPRAFETLVPGNFEIKR
                     MAPEVEPGAPGAYGGPGTIDGKVPGKFWINMHTMSVFTTYNTPTLTYHESLPGHVWQG
                     EYTFKLPLVRSLLAFNAYSEGWALYAEQLANELGVYDEDPFGRLGYLQSIAFRACRLV
                     VDTGLHAKRWTRRQAIDWFASTNGSSV"
     gene            complement(1675..2139)
                     /locus_tag="VEW08_01165"
     CDS             complement(1675..2139)
                     /locus_tag="VEW08_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39363.1"
                     /translation="MTDALMPASAEAPSNKQRLFLRYYTGFLMDLVVLNLFAEYSAKV
                     DVDTFTTSLWAALVLQVLLKVTIIFEHKVLDWFKAKTGALMTFLKYFCAWLILFGSKF
                     VILEALAQLFGDKVRFLGIWHGIVTLIVVVVVMLLAEEIMVRVFRRLADKPD"
BASE COUNT          420 a          731 c          713 g          460 t
ORIGIN      
        1 tccacgctcg atccattggt gctcgcgaac cagtcgatgg cctgccgtcg ggtccagcgc
       61 ttggcgtgca gccccgtgtc caccacgaga cggcacgcgc ggaaggcgat ggactgcagg
      121 taccccagtc gcccgaaggg atcttcgtcg taaacgccga gctcattggc gagctgttcg
      181 gcgtagaggg cccagccttc cgaatacgcg ttgaacgcga gcaacgaccg gaccagcggt
      241 agtttgaagg tgtattcccc ctgccatacg tggccgggaa gcgattcgtg ataggtgagc
      301 gtcggcgtgt tgtaggtggt gaacacactc atggtgtgca tgttgatcca gaacttgccg
      361 ggcactttgc cgtcgatggt gccgggtcca ccgtatgcgc ctggcgcacc gggctccact
      421 tccggcgcca tgcgcttgat ctcgaagttt ccgggcacca gggtttcgaa ggcgcgaggc
      481 agacgtgtgc gaatgtcggc gacgcgagca ttgaggaatg ccatgagctt ggcgcggcca
      541 gcatcacttt cctcgaaccg gaagcgatcc tgcttgccga gcgcggtcat gcgttcgccg
      601 acggttccct ggctcatgcc ctgagttttg aggatgccgt ccatctcgga ctgcaacgcg
      661 cgcaattctt cctggccgcg cgcatggatt tcgtccggcg tcatgcgcgt cgtggttccg
      721 gcacgcagcg tccaggcgta atactcgtcg ccgcgcggca gtttccagac accggcgtcg
      781 ttattggctt gtttgcgatg cacctcgagc tcggcgatct ggcggtcgag cgccggtgcg
      841 accttgtcgg tagccagctt cgcagcgcga gccgcgtaat tgcccggcat gtttttcgtg
      901 cgcttcgcga gcgacgcgac cagcgaccaa tcggccacgt tgccgctgcg cgcgagcttg
      961 atctgcttca gtgtcttgtc gagcagaaaa tccggcgcaa ccacactctg ggccgccgcg
     1021 cttttcagcc gtccggtttc accttcgagc tggcccgcat acgcctccat gcgggcgaga
     1081 taggcgtccg cgtctgcggc atttcccact gtgtgctgct catccagcat gctggggatt
     1141 tccagaaacg cacccgtgtt ctgggccacc acgtacggcg cattgcgcca cgaccactgg
     1201 gagttgagca gcgccacatc gccgtaggga aatgcaaaac cttcgagcgc gaactcatgc
     1261 gcggtgcgca cgacgtcgac atcgattcgc gccgcatttc ccaacctgga cgtgtcgatg
     1321 gacttcagtc ggtcgatccg ttgtgcaacg cgtttcgcga tggccttctg gcccgcggcc
     1381 gagcgatcgc ccagcttcga cttcaaggcg gcgcgggcgc cattgtcgat gccgagctgc
     1441 gtcgcgctct cgggatagtc gaccagcagt tcttcggtga cggctgcaag cagagtctcc
     1501 gcggccttgt cgcccgaggg aatggcgctc ccggcgggta gattgctgac agcaagcaca
     1561 gcgccgctgc ctatgacgaa gtcacggcgc gtatttttga cttccatgac gcacggtaac
     1621 gcgcgcccgg cgccgagcgc cagtgcaatt caacaaccag accagagtcc ggtctcagtc
     1681 gggtttgtcg gcgagccttc ggaagacccg gaccatgatt tcttctgcga gcagcatcac
     1741 gaccaccacg acaatgaggg tcacgattcc gtgccagatg ccgaggaacc tgaccttgtc
     1801 gccgaacaac tgggcgagcg cctcgaggat gacgaacttc gacccgaaca ggataagcca
     1861 ggcacaaaag tatttgagga acgtcatcaa ggcgccggtt ttcgctttaa accagtccag
     1921 caccttgtgc tcaaaaatga tcgtcacctt cagcaacacc tgcaacacca gtgcagccca
     1981 cagcgacgtc gtgaacgtat cgacgtccac tttcgctgag tactccgcga agaggttcag
     2041 caccaccagg tccatgagga agccggtgta atagcggagg aacagccgct gcttgttcga
     2101 gggggcttcc gccgaagccg gcatgagcgc gtcggtcatt gcaatactcc gcaaggcact
     2161 gcggacgagc ctacggcatc tgaaaaattg cgcagttggc cctagggcct acgcgtcagg
     2221 tcgcggcgcc ctcataccac tcggcgtagg ggctgttctt taccttgtgg cgattgaggt
     2281 cgggcgttcc gtcgctccac cagaccgcgc cccgttcgcc gagc
//
LOCUS       DASQJS010000026        12156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3156240, whole genome shotgun sequence.
ACCESSION   DASQJS010000026 DASQJS010000000
VERSION     DASQJS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12156
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3156240"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VEW08_01170"
     CDS             <1..236
                     /locus_tag="VEW08_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYJ39364.1"
                     /translation="NGDVEVRISDNGPGVSPEHLDRLFDPFFTTKRSGTGLGLAISRT
                     IAQGHGGTIGIRPVTPHGASFHVSLPAMENECP"
     gene            233..862
                     /locus_tag="VEW08_01175"
     CDS             233..862
                     /locus_tag="VEW08_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ39365.1"
                     /translation="MKNEVATVMVVDDDAGIRNAMRTLLKSVGLNCTLFASAQEFLGA
                     YQPTQQGCLILDIRMPGMSGLELQQQLNLSGAVIPVIFMTGHGDIPMAVEAMQHGAFD
                     FLQKPFRDQDLLDRIQRAIAKDGELRKSLGEHARIKAHLDSLTPREREVLDLMTKGKQ
                     NKAIAQDLAVSPRTIEIHRARVMEKMNAQSVAELVRMMLDLDHAASGGR"
     gene            904..1395
                     /locus_tag="VEW08_01180"
     CDS             904..1395
                     /locus_tag="VEW08_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ39366.1"
                     /translation="MVGGIEYRIRPIRSDDAERERAFIMSLTPESRFQRLMYTLQEPS
                     AEFVARMVTVDQHRDMALVAVVGGEAEEKIIGVARYAADESGRDCEYAVAVTDEWQCR
                     GIGTTLSKLLFEHAAHEGFRSIYGNVLADNRRMLELAEWLGLSVEPALPGQPTVRASR
                     RLN"
     gene            complement(1392..3950)
                     /locus_tag="VEW08_01185"
     CDS             complement(1392..3950)
                     /locus_tag="VEW08_01185"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HYJ39367.1"
                     /translation="MSPAAGDPTSGLSTAEARARLQREGFNELAAAERRSAWRIATEV
                     VREPMFALLISAAIIYAVLGDRGEALMLLGFATISVSIAIIQQGRSEKALDALRDLTS
                     PRALVIRDGERVRIPGREVVRGDVVVLIEGDRVPADGVLVSGDHLELDESLLTGESIA
                     VRKCATREIPERLAAPGGENSSQVFSGTLVVRGNGLAAILATGMHTAIGAIGASLAGM
                     AREQPRLERQTRRMVVVFGAIGLAVSVLAFLLYWWLRGSWLEALLGAIALGMSLLPEE
                     FPLVLTVFMVMGAWRLSQSRVLTRRAAAIEALGSATVLCTDKTGTLTRNLMSVAHLRT
                     PVEGDLVETWEPGQPPAHIAASASLSQLLRTAALASDVHHRLDPMDRSLADLHDLADP
                     SQPTGTLLREYPLGPELLAVTRVWRFADADTLTVAAKGAPEAIAGLCRFTASQLAGLR
                     LHVDVLAARGMRVLAAAHASSRGANVPETPTSFAFEFLGLIGFADPLRESAPAAIREC
                     RSAGIRVLMITGDYPATATAIAHQAGIEDGATCSGDELRALDDAALAERVRGTSVFAR
                     ISPDQKLRIVNALKANGEIVAMTGDGVNDAPALKAAHIGIAMGGRGTDVAREAASIVL
                     LDDDFGSIVRAVRLGRRIYDNLRKAMGYILAIHVPIAGLALLPILFGWPMVLTPMLIA
                     LLELIIDPACSVVLEAEEEESDVMSRPPRRPDSALLSGALIGWSLLQGGLAFAVVAAV
                     YVLAMLLPVPADEVRSLAFVTLVGANVALIFVNRTFSSSLRAALGRPNRLLAWGMGIT
                     VGLLATIMAWPDVRRFFGLGPLHANDLSLCLAAAAGLLVVLELTKIAWRRRLAS"
     gene            complement(3957..4661)
                     /locus_tag="VEW08_01190"
     CDS             complement(3957..4661)
                     /locus_tag="VEW08_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYJ39368.1"
                     /translation="MNNASKYFILRLAGGLLFSGFAVAASTAQRELADALRATPNAIN
                     GEALFDNCASCHGADGGGQADGSTPRIAGQHFRVLAKQLVDFRSGKRWDFRMEGAADR
                     HHLTGAQDIADVATYVSKLVRPGKRGIGGGEFVEQGSKIYVKQCASCHGPAAEGNAER
                     GVPRLAGQHYGYLMRQMYDAVDGRRPALPRLHSERIAPLDFEQVRAVSDYLARIGWQP
                     DADSEQMAVPEPPQAP"
     gene            4816..5166
                     /locus_tag="VEW08_01195"
     CDS             4816..5166
                     /locus_tag="VEW08_01195"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HYJ39369.1"
                     /translation="MKLDSTNIRFGSRVPHALAVHLSVDARTAGRGTIRNASISGALI
                     ETALDLPLHTNLVLTLSMAVGEVPATRSLAACVVRVDPDGVGIEWRDIAGADVLDLLE
                     RASNLEAVRALPAR"
     gene            complement(5152..5646)
                     /locus_tag="VEW08_01200"
     CDS             complement(5152..5646)
                     /locus_tag="VEW08_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39370.1"
                     /translation="MINRSVASGFLMCVALAAAGCTCTSDGLELDSRRETEKTSVTFE
                     LRRTDGVSGSITALFADGRQFHGEYLQITREARLAQLTPLWEGWTFGWGWPHWRPQAA
                     PKFVKHHDGEVLANLASVNGERMRCRFDLENRSLGVSGGGEGECEFPGGRTVDARILP
                     STRG"
     gene            5876..6250
                     /locus_tag="VEW08_01205"
     CDS             5876..6250
                     /locus_tag="VEW08_01205"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HYJ39371.1"
                     /translation="MGQSANAQMASQPRPAFMEHRWGQRMRCLARVRLSAGAENTGAG
                     RVRDVSSSGAYIETSFDIPVHARVTLLVQGNESSTRVVEIAASVARRDREGIGVEWCD
                     TPGCSICVVLGCTTPCAASKET"
     gene            6253..6525
                     /locus_tag="VEW08_01210"
     CDS             6253..6525
                     /locus_tag="VEW08_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HYJ39372.1"
                     /translation="MSRAKFARHSKTTTAGVPMEIANFAQDEIEESFERIDENGDRSI
                     SFGEFSRLMLDLDHARPESALRVSFDAIDKDHDGRVSFDEFRAWCK"
     gene            6570..7832
                     /locus_tag="VEW08_01215"
     CDS             6570..7832
                     /locus_tag="VEW08_01215"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HYJ39373.1"
                     /translation="MSYPPIMLASMNNQFEAAQPADVSFPEYDSARRCALFDYGFRPF
                     FLLCGAYALIMIPWWMYRFAHASVSFGGLPAVYWHAHEMIYGFVMAAIAGFLLTAVPS
                     WTSQRGSAGLPLIVAVALWTAGRVAMSAVGAVPFWLVAATELALVPCLLALLAPPILR
                     TRNRNLPILAVLVVLWIIDGAFLLSLARGDVLLAAGAIRLAIDFVLVLVTIIGGRIVP
                     TFTASALRRRGETVSTVTRKPLEYAVIGLMIAIAVTDVFAPQLWLSGVLAALAALAHV
                     LRLAGWRSFRTRGEPILWIMHVAYAWIPLGLALKAFALLGDFAWAAKWQHAFGTGVLA
                     TMILAVMTRVALGHTGRPLVVSRAIGIAYLLLTFAALLRVFGVALFPAHYLMTLTIAG
                     LAWVLSFGIFLVVYTPIFWWPRADGKPG"
     gene            7829..9229
                     /gene="hemN"
                     /locus_tag="VEW08_01220"
     CDS             7829..9229
                     /gene="hemN"
                     /locus_tag="VEW08_01220"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HYJ39374.1"
                     /translation="MSTQLQFDPALLRRYDRPGPRYTSYPSAPRFHQDFTEADLRRHA
                     ARSNEEPIPRDLSLYVHVPFCFSPCFYCGCNRIITRDATRGPPYVERLLREAELLSRL
                     FDRDRGVSQVHFGGGTPNFLDAPTLRGLIDGLARHFRFSTGDDRDFSIELDPRTVREG
                     DIAAYADMGFNRASFGVQDFDRTVQLAINREQSVAQTLSALEECRNSGFRSVNVDLIY
                     GLPRQNVAGFCRTLDIIADARPDRVAVYGYAHIPQMFKAQRQIAEQDLPDAATRLDLL
                     QAAIEKLTAAGYVYIGLDHFALPEDELSLALDAGTLQRNFMGYTTHAACDLIGLGVSA
                     ISHVGNSFSQSHRDLTAWEVAVDQGRLPVHRGLTLDDDDIVRADVIQELMCSGAVDRA
                     RIARRYDIDFDEYFSEALTQLAPLAEDGLVESSAERIVATSRGRLMLRIIAMCFDRYL
                     SAQPASEIRPRHSRAF"
     gene            9263..10006
                     /locus_tag="VEW08_01225"
     CDS             9263..10006
                     /locus_tag="VEW08_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ39375.1"
                     /translation="MAGPHGKGNPIADDGDAFHFCSTCAFSAACLAQGYDKSKLEELH
                     VLVAHVGPFGEGEHIFREGDPFEAIAAVRVGTVKTYVIDSEGREQVQGFFLPGEVIGL
                     NAISQARYPCNAVALDSVMLCRFSFPNIAALASRVPGLQQHLFRLLSEDIGKAALLAG
                     NFTADERMAAFVVSMSRRHAARGFSATRFRLSMTRTDIANYLRLASESVSRGFSRLQD
                     AGLLRVDRREIEILDPARLDATARNILRD"
     gene            10149..>12156
                     /locus_tag="VEW08_01230"
     CDS             10149..>12156
                     /locus_tag="VEW08_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HYJ39376.1"
                     /translation="MTATRKLWLFLAGLLVTSFTVLLWVGGEIHRVMPPVPSAVVTTD
                     GRTLYTRADIEQGRQVWQSIGGHQLGSIWGHGSYVAPDWSADWLHREATEWLNITARS
                     QTSLDYASLPADQQAALRARFQPLIRANTYDVETDLITLAPERAAAMATVAAHYEALF
                     GTDPRLLELRKAYAMKQQTVDTAEHRHALTAFIWWTSWSAVTERPGKTISYTNNWPHD
                     PLVGNTPPGHLFMWTVFSVLFLIAGIAALGWHHARNKHEAPLELPKTDPLAMVRVTPS
                     MKASAKYFWLVIALFLTQILLGAITAHYQVEGQDAYGFALSNYLPYSLSRTWHTQLAV
                     LWIATAWLGMGLYIGPAISGHEPKYQRLGVNVLFACLLIIVVGAFTGQWLAVMQKLGL
                     AKNFWFGHQGWEYVDLGRFWQIFLFAGLGIWLTLVGRALMPAIRRGGESKSIIALLFA
                     STVCIGLFYGAGLMWSEHTHLSMVEYWRWWVVHLWVEGFFEVFATAVIAFLFTRLGLL
                     RVKTATVAVLFATIVFLSGGVLGTLHHLYFVGTPIAVVALGASFSALEVVPLAYIGFE
                     AYHTWKLGHATPWMQRYRWPIMFFTAVAFWNVVGAGLFGFLINPPLPLYYMQGLNLTP
                     LHGHTALFGVYGFLGIGLMLFCLRGLKPQAIWPEAPLRTCFWALN"
BASE COUNT         2157 a         3930 c         3838 g         2231 t
ORIGIN      
        1 tgaacggtga cgtcgaggtc cggatttcag ataacggccc gggcgtttcg ccggagcatc
       61 tggatcgcct gttcgatcca ttctttacga ccaagaggtc cggcacgggc ctgggcctgg
      121 cgatcagccg taccatcgcg caggggcacg gcggtactat cggcatccgg ccggtaacgc
      181 cccatggggc aagcttccac gtgagcctgc cggcgatgga gaacgaatgt ccatgaagaa
      241 cgaagttgca accgtgatgg tagtcgacga cgacgcggga atacgcaacg ccatgcgtac
      301 gctgctcaag tcggtaggcc tcaactgcac gctgtttgca tccgcgcagg agttccttgg
      361 cgcatatcaa ccgacgcagc aaggatgcct gattctcgac atccgcatgc cgggcatgag
      421 cgggctggag ttgcagcaac agctgaatct gagcggcgcg gtcattccgg tcattttcat
      481 gaccgggcac ggtgacatac ccatggcggt cgaagccatg cagcatggcg cgttcgattt
      541 cctgcaaaag ccctttcgcg atcaggatct cctcgaccgt atccagcgag ccatcgcaaa
      601 agacggcgag ctgcgcaaat cactggggga acacgcgcgc atcaaggcgc acctggattc
      661 cctgacgccg cgcgaacgcg aggtgctcga cctgatgacg aagggcaaac agaacaaagc
      721 catcgcccag gacctggccg tcagcccgcg caccatcgag attcatcgcg cccgagtcat
      781 ggagaaaatg aatgcgcagt cggtggcgga gctggtgcgc atgatgctcg acctggatca
      841 cgctgcatcc ggcggccggt gagcccgaac tcaccaccgc cagcctggcc gcgcgccgcc
      901 tgcatggttg gcgggatcga atatcgcatc cggcccatac gcagcgacga tgcggaacgc
      961 gagcgggcat tcatcatgag cctcacgccc gagtcacgct tccagcgtct catgtacaca
     1021 ctgcaggaac cgtccgcgga attcgtcgcg cgcatggtca ctgtcgatca gcatcgcgac
     1081 atggctctcg tggcggtagt gggcggcgag gcggaagaaa agatcatcgg cgtagcgcgc
     1141 tacgcggcgg acgagagcgg gcgagactgc gagtacgcgg tggcggtgac cgacgaatgg
     1201 cagtgccgcg gcatcggcac cacgctctcg aagctgttgt tcgagcatgc cgcacacgag
     1261 ggcttcaggt ctatctacgg caatgtgctt gccgacaatc gccgcatgct cgagctcgcc
     1321 gaatggctgg gtctcagcgt ggagccggcg ttgcccggcc aaccgacggt acgtgcgtcg
     1381 cggcgcctca actaactcgc gagccggcga cgccaggcga tcttggtgag ctccagcacc
     1441 acgagcagac ctgcggcagc cgcaagacat agcgacagat cgttggcgtg caacgggccg
     1501 agcccgaaga accgacgcac atccggccac gccatgatcg tggccagcaa accaaccgtg
     1561 atacccatac cccacgcgag caatcgattc ggccggccga gcgcggcccg cagcgatgac
     1621 gagaacgtgc ggttgacgaa aatcagcgcg acgttcgcac cgaccagcgt gacgaaagcg
     1681 agcgagcgca cctcatcggc cggcacgggc aacagcatgg cgaggacgta cacggccgcg
     1741 accaccgcaa aggcgagccc gccttgcagc aggctccatc cgatgagcgc accggacagc
     1801 agggcgctgt cggggcggcg cggtggacga ctcatgacat cgctctcttc ctcttccgcc
     1861 tcgaggacca ccgagcaggc cggatcgatg atgagttcga gcaatgcgat gagcatcgga
     1921 gtcagaacca tgggccaacc gaacaggatg ggcagcaggg cgagaccggc gatcggcacg
     1981 tggatggcca gaatatagcc catggctttc cgcaaattgt cgtagatacg ccgcccgaga
     2041 cgcaccgcac gaacgatgga accgaagtca tcgtccagca acacgatgga cgcagcctcg
     2101 cgcgccacgt cggtgccacg gccacccatg gcgatgccga tatgagccgc cttgagcgcc
     2161 ggggcatcgt tcacgccgtc cccggtcatc gccacgattt cgccattggc cttcagcgca
     2221 ttcacgattc gcagcttctg gtccggtgaa atccgcgcga acaccgaagt tccgcgcacg
     2281 cgctcagcca gcgcggcgtc gtcgagcgcg cgcagttcat cgccggaaca cgttgcgccg
     2341 tcctcgatgc ccgcctggtg agcgatggca gttgcagtgg ccggataatc gccggtgatc
     2401 atcagcacgc ggattcccgc cgagcgacat tcgcggatcg cggcgggcgc cgactcgcgc
     2461 aacggatccg cgaagccgat gaggccgaga aattcaaagg cgaacgaggt tggcgtctcg
     2521 ggcacgttcg caccacgaga actcgcgtgc gccgcggcca acacgcgcat tccgcgtgcc
     2581 gccagcacgt caacatgcaa gcgcaacccg gcgagttgcg acgccgtgaa cctgcacagc
     2641 ccggcaattg cttcgggcgc gcccttcgca gccacggtca gcgtatccgc gtctgcgaag
     2701 cgccacacgc gcgtcactgc aagcagttcg ggaccgagcg gatattcccg gagcagcgta
     2761 ccggtgggct gcgagggatc cgcgagatcg tgcaagtccg cgagcgaacg gtccatggga
     2821 tcgagccgat gatggacatc gctcgccaga gctgccgtcc tgagcaattg cgacagcgaa
     2881 gccgaagcgg cgatatgcgc gggcggctgc ccgggctccc aggtttcaac cagatcgcct
     2941 tcaaccggcg tgcgcagatg tgccactgac atgaggttgc gcgtcaacgt tccggtctta
     3001 tcggtgcaca acaccgtcgc cgaacccagc gcctcgatgg cggcggcacg acgcgtgagt
     3061 acgcgcgact gcgacaatcg ccaggcgccc atcaccatga agacggtgag caccagcgga
     3121 aattcttcgg gcagcagcga catgccgaga gcaatcgcgc cgagcaacgc ctcgagccag
     3181 gacccgcgca gccaccagta caacaggaag gcgagcacgc tcacggcgag cccgatcgcg
     3241 ccgaagacca cgaccattct gcgggtctga cgctcgaggc gcggctgctc gcgcgccatg
     3301 cctgcaagcg acgcgccaat cgctccgatg gcggtgtgca tgccggtcgc gagtatggcg
     3361 gcgagcccat tgcccctgac gaccagtgtc ccggaaaaaa cctgcgacga gttttcgcct
     3421 cctggcgccg caagcctctc gggtatttcg cgcgtggcgc atttacgtac cgcaatggat
     3481 tcgcccgtca gcagcgattc atcgagttcg aggtgatctc ccgataccag gaccccgtcg
     3541 gcgggcaccc gatcgccctc gatgagcacg actacgtccc cgcgcaccac ctcccgtccc
     3601 ggaatgcgaa cgcgctcgcc gtcgcggatc accagggcgc gcgggctggt gagatcgcgc
     3661 aatgcgtcaa gcgccttctc gctgcggccc tgctggatga tggcgatgga cacggaaatg
     3721 gtcgcgaagc cgagcagcat cagggcttcg ccacggtcgc caagcaccgc gtaaatgatg
     3781 gcggcggaaa tcagcagcgc gaacattggc tcgcgcacga cttccgtcgc gatgcgccat
     3841 gccgagcgtc gctccgccgc ggccaattcg ttgaatcctt cccgttgcag ccgcgcgcgt
     3901 gcctcggcgg tgctcaatcc cgatgttgga tcgccagccg cgggtgacat cggccgtcag
     3961 ggagcttgag gaggctcggg cacggccatt tgttcggagt ctgcgtccgg ctgccagcca
     4021 atgcgcgcca ggtagtcgga aacggcgcgc acctgctcga agtcgagtgg cgcaatgcgc
     4081 tcggaatgga gccgcggaag cgccgggcgc cgcccatcga cggcgtcgta catctgccgc
     4141 atcaggtatc cgtagtgctg gccggcgagg cgcggcacgc cgcgttccgc atttccctcg
     4201 gcggcgggtc catggcacga cgcgcactgc ttcacgtaga ttttgcttcc ttgctcgacg
     4261 aattcgccgc cgccgatacc gcgctttccc ggtcggacca gcttgctcac gtacgtggcg
     4321 acatcggcga tgtcctgtgc gccggtgaga tgatggcggt cggcggcgcc ctccatgcgg
     4381 aaatcccagc gtttgccgga gcggaagtcg acgagttgct tggcgagcac gcgaaagtgc
     4441 tgcccggcaa tgcgcggcgt cgagccgtcg gcttgtccac ctccatccgc accatggcac
     4501 gaggcgcaat tgtcgaaaag cgcctcacca tttatcgcgt tgggagtggc ccgcagcgcg
     4561 tcggcgagct cgcgttgcgc ggtggacgca gcgactgcga agccagaaaa cagcaggccg
     4621 ccggcaagtc gcagaatgaa atacttcgat gcgttgttca tttcagtgtt ctatcaactg
     4681 gtatcgccag ctcatatacg tgatagaccg cataggactc agggttgctc gcagcttgtc
     4741 tcggcaacgg gtgcgtagtc cccctcattc cgcccgatgt cccgttcgtc ctacaattgc
     4801 cgttggacga actagatgaa acttgactcg acgaacatcc gcttcggatc gcgtgttccg
     4861 cacgcgctgg cagtgcatct gagtgtcgac gcccggacgg caggacgggg aacgattcgc
     4921 aatgccagca tcagcggcgc cctcatcgag acggcattgg atctgccgct gcacaccaac
     4981 ctcgtcctga cgctgtcgat ggcggttgga gaagtgcctg cgacccgttc tctcgccgca
     5041 tgcgtcgtgc gtgtcgaccc ggacggcgtg ggcattgaat ggcgcgacat cgcaggcgcc
     5101 gacgtcctgg atctgctcga acgagcctcc aacctcgaag cggtgcgcgc cctacccgcg
     5161 cgttgaaggc aagatcctgg cgtcgacggt cctgccgccc gggaactcac actcgccttc
     5221 accgccgccg ctcacgccca gcgagcggtt ttcgagatcg aaccggcagc gcatgcgctc
     5281 gccgttcacc gaagcgaggt tggcgagaac ttcgccatcg tgatgcttca caaatttcgg
     5341 cgcggcctgc gggcgccaat gcggccaccc ccagccaaac gtccatcctt cccacaatgg
     5401 cgtgagttga gcgaggcgcg cttcccgcgt gatctgcaga tattcgccgt ggaactgtcg
     5461 gccgtcggca aatagcgcgg tgatgctgcc cgaaacgccg tcggtacgcc gcaattcgaa
     5521 cgtgacggaa gttttttcgg tttcccgccg ggaatccagc tcgagaccat cagacgtgca
     5581 agtgcagccg gccgccgcga gtgccacgca catcaagaat ccactcgcga cacttcgatt
     5641 gatcactggc gacttccttt gcggtggagc cttgcagatc aaacggtctc gcgcatgagt
     5701 gagatccctg acactcgtaa gcaggttcaa cgcatggccc gcctatcgag cttcggctgc
     5761 tcgtgttgct gaatatgcgt agttctcctc atgacacgcg gtttcctccc gatgccgagg
     5821 cgggcatcgc cgactgacaa ttcagtcgac gaacggtcat tcgcaggaga gcgtcatggg
     5881 gcagtcagca aacgcacaaa tggcgagcca gccacgaccc gctttcatgg aacatcgctg
     5941 ggggcagcgc atgcgatgcc tggcacgagt acggctgtcc gcaggtgccg aaaacactgg
     6001 cgcaggtcgc gtgcgcgacg tcagctccag cggcgcttac atcgagacct cgttcgatat
     6061 tcctgtacat gcgcgcgtga cactgctggt ccagggcaat gaatcgtcga ctcgcgtggt
     6121 cgaaatcgcc gcgagcgtgg cgcgcaggga ccgcgagggt attggtgtcg aatggtgcga
     6181 cacaccgggg tgctctatct gtgtagtgct cgggtgtacc actccctgtg ccgcatcgaa
     6241 ggaaacatga cgatgagccg cgcgaaattt gcgcggcatt ccaaaaccac caccgccgga
     6301 gttcccatgg aaattgccaa ttttgcgcag gatgaaatcg aggagtcgtt tgaacgcatc
     6361 gacgagaacg gggatcgcag catcagcttc ggggagttct ccaggctcat gctcgacctg
     6421 gaccatgcca ggccagagtc tgcgctgcgc gtgagtttcg acgcgatcga caaggaccac
     6481 gacggccgcg tgagcttcga cgagtttcgt gcctggtgta agtagtcagt gcgcgttcca
     6541 tggcctcgag ctgatctgga tcagagtgaa tgtcgtatcc accgatcatg ctggccagca
     6601 tgaacaatca gttcgaagct gcgcaaccgg ccgacgtatc gtttccggaa tacgactccg
     6661 cccgtcgctg cgccttgttc gattacggct tccggccttt cttcctgctc tgtggcgcgt
     6721 acgcgctgat catgattccg tggtggatgt accgcttcgc acacgccagc gtatcgttcg
     6781 gcggattgcc ggcggtctac tggcacgcgc acgaaatgat ctatggcttc gtgatggcgg
     6841 cgattgccgg gttcctgctc acggcggtcc cgagctggac atcccagcga ggctccgcgg
     6901 gtttgccatt gatcgtcgcg gttgcgttgt ggaccgccgg ccgggtggca atgagtgcgg
     6961 tgggcgccgt tccgttctgg ctggtcgcag cgacggagct ggcgctcgtg ccttgcctgc
     7021 ttgcgctgct ggccccgccc atcctgcgta cgcgcaatcg caatctcccg atcctcgccg
     7081 tactggtcgt gctctggatc atcgacggcg ccttcctgct gagcctggca cgtggcgatg
     7141 tattgctcgc tgcgggagcg attcgcctgg ccatcgattt cgtgctggtg ctggtcacga
     7201 tcatcggtgg acgcatcgtg ccgacgttta cggccagcgc actgcgccgg cgcggcgaga
     7261 ccgtgagcac agtcacgcgc aagccgctcg agtacgcggt gatcggcctc atgatcgcga
     7321 tcgcggtgac cgacgtattc gcgccgcagc tctggctcag cggcgtgctc gccgccctcg
     7381 cagccctggc gcacgtgttg cggctcgccg gctggcgcag ttttcgcacg cgcggtgaac
     7441 ccatcctctg gatcatgcac gtcgcatatg catggattcc gctcggactc gcgctcaagg
     7501 catttgcgct gctgggtgat ttcgcctggg ccgccaagtg gcagcatgcg ttcggcacgg
     7561 gcgtgctggc gacgatgatc ctcgcggtca tgacgcgcgt tgcactcggg cacaccggcc
     7621 gcccgctggt ggtttcgcgc gcgatcggaa ttgcttacct gctgctcacg tttgcggcgt
     7681 tgctccgtgt gttcggcgtg gcgctgttcc ccgcgcacta tctaatgacg ctgaccatcg
     7741 cgggcctcgc gtgggtgctc tcgttcggca tcttcctcgt ggtgtacaca ccgatcttct
     7801 ggtggccgcg cgcggacggg aaacccggat gagcacacag ctgcaattcg atccggcact
     7861 gctgcgtcga tacgaccggc cagggccgcg atacacgtcc tacccatcgg cgcccaggtt
     7921 ccaccaggat ttcaccgaag cggacctgcg ccggcacgcg gcgcgcagca acgaggagcc
     7981 gatcccgcgc gacctctcct tgtatgttca tgtaccgttc tgcttctcgc cgtgttttta
     8041 ctgcggctgc aaccgcatca tcacgcgaga tgcgacccgt ggcccgccct acgtggaacg
     8101 cctgctgcgc gaggcggagc tgctctcgcg cctgttcgac cgcgaccgcg gagtgtcgca
     8161 ggtgcacttc gggggcggga cgcccaattt tctcgacgca ccgacgctgc gagggctcat
     8221 cgacggcctc gcgcgccact tccgcttttc cacgggcgac gaccgggatt tctccatcga
     8281 gctcgatccg cgcaccgtgc gcgagggcga tatcgccgca tatgcggaca tgggattcaa
     8341 ccgcgcgagc ttcggcgtgc aggacttcga ccgcacggtg cagctggcca tcaaccgcga
     8401 gcagagcgtc gcgcaaacgt tgagcgcgct cgaggagtgc cgcaactcgg gcttccgttc
     8461 ggtgaacgtg gatctcatct atggcctgcc gcgccagaac gtggccggat tctgccggac
     8521 actcgacatc attgccgatg cgcgtcccga tcgggttgcg gtctacggct acgcgcacat
     8581 cccgcagatg ttcaaggcgc agcgccaaat cgccgagcag gatctgcccg acgcggcgac
     8641 gaggctcgat ctgctgcagg cggcgatcga aaagctcacc gcggcgggct atgtgtacat
     8701 cggtctcgat cacttcgcgt tgccggagga cgaattgagc cttgcgctcg acgcgggaac
     8761 attgcagcgg aatttcatgg gctacaccac acatgctgcc tgcgatctca tcggccttgg
     8821 tgtcagcgcc atcagccacg tcggcaacag cttcagccag agtcatcggg atctgacggc
     8881 atgggaagtg gccgtggacc agggccggct gccggtgcat cgcggcctca cgctcgatga
     8941 cgacgacatc gtccgcgccg acgtgatcca ggagctgatg tgcagcggcg cagtcgatcg
     9001 cgcccgcatc gcccggcgct acgatatcga tttcgacgaa tacttttccg aagccctcac
     9061 gcagctcgcg ccgctggccg aagacggcct cgtggaatcc tccgcggagc gcatcgtcgc
     9121 aacctcgcgc ggccggctga tgctgcgtat catcgcgatg tgcttcgacc gctatctttc
     9181 agcgcagccg gcttccgaaa tccgcccacg ccattcacgc gcgttctgac gtttcgccgc
     9241 ttggcgcggg cgagcgcctg acatggcggg cccgcatgga aagggaaatc cgatcgccga
     9301 tgacggcgat gcgtttcact tctgcagcac ctgcgcgttt tccgcggcgt gcctcgcgca
     9361 gggatacgac aagtcgaagc tggaagagct gcacgtgctg gtcgcgcatg tcgggccgtt
     9421 cggggaaggc gaacatatct ttcgggaagg cgatccgttc gaggcaatcg cggcagtccg
     9481 tgtgggcacc gtaaagacct acgtgatcga cagcgaaggc cgcgaacagg tccagggttt
     9541 cttcctgccg ggcgaagtga tcggtctcaa tgcgatttcg caggcgcgtt atccctgcaa
     9601 tgcggtggcc ctggattcgg tcatgttgtg ccggttctcg tttccgaaca tcgccgccct
     9661 cgccagccgc gtacccggat tgcagcagca tttgttccgg ctgctgagcg aggacatcgg
     9721 caaggcggcc ctgctcgccg gcaatttcac ggccgacgag cgcatggccg cgttcgtcgt
     9781 gtccatgtcg cgcaggcatg ccgcgcgcgg tttctccgcg acgcgattcc gcctttcgat
     9841 gacgcgcacc gacatcgcca actacctgcg cctggcatct gaaagcgtga gccggggttt
     9901 ctcgcgcctg caggatgccg gcctgttgcg cgtcgaccgg cgtgagatcg agatcctcga
     9961 tccggcgagg ctggatgcga cggcgcggaa tattcttcgc gactgagcaa tattcacgcc
    10021 gacatttcgc aatgtgccgg tgccataggt agtgctactg acctcgcgtc ccgccttaac
    10081 ctgatctgga tcacggtccg cgtttctgcg accacgcacg atgacgcatc gttcgaggag
    10141 atgacgtcat gaccgcgact cgcaaactct ggctcttcct ggctggcctc ctggtcacct
    10201 cattcacggt gctgctgtgg gtaggaggtg aaatccatcg tgtgatgccg ccggtcccga
    10261 gtgctgtggt gacgacggat gggcggacac tttacacgcg tgccgacatc gaacaggggc
    10321 gccaggtgtg gcaatccatt ggtggccatc agttaggctc catctgggga cacggaagct
    10381 acgtcgcacc cgactggtcc gcggactggt tgcatcgtga ggccaccgaa tggctgaaca
    10441 tcacggcccg gagccagacc tcactcgatt acgcctcact accggccgac cagcaggccg
    10501 cgttgcgcgc gagatttcag cccctgatcc gcgccaacac ctacgacgtg gagaccgacc
    10561 tcatcacgct ggcgcccgag cgcgcggcgg ccatggccac cgtggccgcg cactatgaag
    10621 cgctgtttgg caccgatccg cgcctgctcg agctgcgcaa ggcgtacgcg atgaagcagc
    10681 agacggtgga cacggcggag catcgccacg cactcaccgc tttcatctgg tggacttcat
    10741 ggtccgcggt caccgagcgc ccgggcaaga ccatcagcta caccaacaac tggccgcacg
    10801 atccgctggt cggcaatacc ccaccgggtc acctcttcat gtggacggtg ttcagcgtgt
    10861 tgttcctcat cgctggcatc gccgcgcttg gctggcatca cgcgcgcaac aaacacgaag
    10921 cgccgctgga attgccgaag accgatccgc tcgcgatggt tcgcgtaacg ccctccatga
    10981 aggcgagcgc gaagtatttc tggctcgtga tcgcattgtt cctgacgcag atccttctcg
    11041 gggcgatcac cgcgcattac caggtagagg gccaggatgc ctacggattc gcactgtcaa
    11101 actacctgcc ctactccctg agccgcacct ggcacacgca gctggcggtg ctgtggatcg
    11161 cgacggcctg gctaggcatg ggcctgtaca tcggccccgc catttccggt catgagccga
    11221 aataccagcg gctcggcgtg aacgtactgt tcgcgtgcct gctgatcatc gtggtcggcg
    11281 ccttcacggg ccagtggctg gcggtgatgc agaagttagg actggcgaag aacttctggt
    11341 tcggccacca gggctgggag tacgtcgacc tcggccgctt ctggcagatc ttcctgttcg
    11401 cgggactcgg catctggctc acgctggtgg gacgcgcgct catgcccgcc atcaggcgag
    11461 gtggtgaatc gaagtccatc atcgccctgc tgttcgcatc cacagtctgc atcggcctct
    11521 tctacggtgc cggactcatg tggagcgagc acacgcacct ctccatggtg gagtactggc
    11581 gctggtgggt ggtgcacctg tgggtggagg ggttcttcga ggtgttcgcc accgcggtga
    11641 ttgcctttct gttcacgcgg ctcgggttgc tgcgggtgaa gaccgcaacc gtggccgtgc
    11701 tgttcgcgac catcgtgttc ctgtccggcg gcgtgctcgg cacgttgcat cacctgtact
    11761 tcgtcggcac tccgatcgct gtcgtcgcct taggcgcgag cttcagcgcg ctggaagtcg
    11821 tgccgctggc ctacatcggc ttcgaggcct atcacacgtg gaagctcggg catgccacac
    11881 cgtggatgca gcgctatcgc tggccgatca tgttcttcac cgcagtggcg ttctggaacg
    11941 tggtcggcgc gggactgttc ggcttcctga tcaacccgcc gctgccgctg tactacatgc
    12001 agggattgaa tctcacgccg ctccacggcc acaccgcgtt gttcggtgtg tacggcttcc
    12061 tcggcatcgg cctgatgctg ttctgcctgc gcggcctgaa gccgcaggcg atctggccgg
    12121 aggcgccgct gcgcacctgt ttctgggcgc taaaca
//
LOCUS       DASQJS010000027         3015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1901611, whole genome shotgun sequence.
ACCESSION   DASQJS010000027 DASQJS010000000
VERSION     DASQJS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901611"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VEW08_01235"
     CDS             <1..455
                     /locus_tag="VEW08_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39377.1"
                     /translation="TDHHREARRTAIVRLLRHSPVRRQQELVRLLKREGHAVTQSSIS
                     RDLRELGVLKASDGYVLPDKAELSSRTQDKFSAVASFVREVRTAGPSITVVKTTIGSA
                     GSVAAAIDKAGWNEVAGTVSGDDTIFIATADARAQARVLEQLREAFGV"
     gene            462..1676
                     /gene="argG"
                     /locus_tag="VEW08_01240"
     CDS             462..1676
                     /gene="argG"
                     /locus_tag="VEW08_01240"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HYJ39378.1"
                     /translation="MSAVNSDKPIVLAYSGGLDTSFLVPWLKENHGRPIITVTVDTGG
                     IDAAGAKTLAERSKAYGAIAHHQIDARADYFEQVLRFLIMGNVRRGQLYPLCVGAERV
                     MQAQQIALMARKLGTNSIAHGCTAAGNDQVRFEVAMRTLAPELEVIAPVRDKAFKRQE
                     ELDYLNARKLPLPPYGAAYSINRGLWGVTIGGKETLTSEGSIPDNAWVLSKDAFTNPR
                     APAKHTIHFEKGRPVGIDGKTMSAVDVVEQLETLGAPYAIGRGIHLGDTIIGTKGRVA
                     FEAPAAETLLTAHRELEKLVLTPRQQRIKESLAGPYGDWVHEGQLLDPVCRDIEALLL
                     SSQERVTGKVHVLFRPGSLFIEGVESKHSLMAVSKGVYGESHGEWTPQDALGFSKIVS
                     LTGMFHARAGGK"
     gene            1676..2761
                     /locus_tag="VEW08_01245"
     CDS             1676..2761
                     /locus_tag="VEW08_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39379.1"
                     /translation="MRTVVVDKIASVTQACGLGNEVRVSTENIPSEEGVVVVVEILTN
                     KSTYNTLELTSGRMAKVGKGDIVAGALGHRKALFGYSGHVPESVKPGDIIQMLNIGGV
                     LGIVDSVNPDKGKPFDCRVIGGVLHFPYLGERIGVPARVGYRQLDYAAKLATNGVPVV
                     ALAGTCMEAGKTAAAAAIISRMRHRGLVVDAFKATGVSLRRDIMAFEDAGARNTLIFT
                     DFGIVSTTRKVGPALTRTMLTELAEKKPDVIVFELGDGLLGTYGVDSILEQEDIRKAL
                     TGVVLSANDPVAAYGGVDILRKRLNIEPVAVTGPATDNEVGVAIIRDQLKVPAFNALA
                     AGAALGDSIIESVGLTQARPLDRAANE"
     gene            2780..>3015
                     /locus_tag="VEW08_01250"
     CDS             2780..>3015
                     /locus_tag="VEW08_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39380.1"
                     /translation="MANKIPAIVLGGTGYVSGELLRLIIGHPDFTLAGVMSDSSPGEI
                     AGKSFPHLAGALGTTAFKSQAEIAALIAKEPTSAV"
BASE COUNT          541 a          975 c          975 g          524 t
ORIGIN      
        1 tcacggacca tcaccgcgag gcgcgtcgca ccgccatcgt tcgcctgctg cgccactctc
       61 cggtgcgccg ccagcaggag ctcgtgcgcc tgttgaagcg cgaaggccac gcggtcacgc
      121 agtcttccat cagccgcgac ctgcgcgagc tgggcgtgct caaggcaagc gacggctacg
      181 tgctgccgga caaggcggag ctctcctcgc gcacccagga caaattttcg gcggtggcgt
      241 cgttcgtgcg tgaagtgcgc accgccggcc cgtcgatcac ggtcgtgaaa accaccatcg
      301 gttcggccgg cagcgtcgcg gctgccatcg acaaggccgg ctggaacgaa gtcgcgggca
      361 cggtatccgg cgacgacact attttcatcg ccacggcgga cgcgcgtgct caagcgcgcg
      421 tgctcgaaca gttgcgcgaa gcttttggag tctgaataga aatgagtgct gtgaactccg
      481 acaaaccgat cgtactcgcc tattcaggtg ggttggacac ctcgttcctg gtgccgtggc
      541 tcaaggaaaa ccatggccgt ccaatcatca cggtcacggt cgacacgggt ggcatcgatg
      601 ccgccggtgc aaagacgctt gcggagcgtt cgaaggccta cggcgcgatc gcgcaccacc
      661 agatcgacgc gcgtgccgac tatttcgagc aggtgctgcg cttcctcatc atgggaaacg
      721 ttcgccgcgg ccagctctat ccgctgtgcg tcggcgcgga gcgtgtcatg caggcgcagc
      781 agatcgcgct gatggcgcgc aagctcggca cgaattcgat tgcgcacggc tgcaccgcgg
      841 ccggcaacga ccaggtccgc ttcgaagtgg ccatgcgtac gctcgcgcca gagctcgagg
      901 tgatcgcgcc ggtgcgcgac aaggcgttca agcgccagga agagctggat tatctgaacg
      961 cgcgcaagct gccattgccg ccctatggcg ccgcgtattc catcaatcgc ggcctgtggg
     1021 gcgtgaccat cggtggcaag gaaacgctga cgtccgaagg cagcattcct gacaatgcct
     1081 gggtgctgtc gaaggacgcg ttcacgaacc cgcgcgcgcc cgcgaaacac accatccact
     1141 tcgagaaggg ccggcccgtg ggcatcgacg gcaagacgat gtcggccgtg gatgtggtcg
     1201 aacagctcga aacgctcggt gcgccgtacg ccattggccg tggcattcac ctgggcgaca
     1261 ccatcatcgg caccaagggc cgggtcgcgt tcgaagcccc cgccgccgag acactgctca
     1321 ccgcgcatcg cgagctcgag aaactcgtgc tcacgccgcg tcagcagcgc atcaaggaat
     1381 cgcttgccgg tccatacggc gactgggtgc acgaaggcca gttgctggat ccggtgtgcc
     1441 gggacatcga agcgctgctg ctgtcgtccc aggagcgcgt cacgggcaag gtccatgtgc
     1501 tgttccgccc cggtagtttg ttcatcgagg gcgtcgagtc gaagcactcg ctcatggcgg
     1561 tgtccaaggg cgtctatggc gagtcccatg gcgagtggac gccgcaggac gcgctgggtt
     1621 tttcaaagat tgtttcgctc acgggcatgt ttcatgcgcg tgccggagga aagtgatgcg
     1681 taccgtagtc gtcgacaaga ttgcttccgt tacccaggcc tgcggcctcg gcaatgaggt
     1741 gcgcgtttcc accgagaaca tcccttccga ggaaggcgtg gtcgtggtcg tggaaatcct
     1801 gaccaacaag tccacctaca acacgctcga gctcaccagc ggccgcatgg ccaaggtagg
     1861 caagggcgac atcgttgcgg gcgcgctcgg ccatcgcaag gcgctgttcg gctattcggg
     1921 ccacgtcccc gagagcgtca aacccggcga catcatccag atgctcaaca tcggtggcgt
     1981 gctcggcatc gtcgattcgg tgaaccccga caagggcaag cctttcgatt gccgcgtcat
     2041 cggcggcgtg ctgcatttcc cgtatctcgg cgaacgcatc ggtgtgccgg cgcgtgtcgg
     2101 ttatcgccag ctcgactacg ccgccaagct cgccaccaac ggcgtgccag tggtcgcgct
     2161 cgcaggcacc tgcatggagg ccggcaagac cgccgcggcg gccgccatca tttcgcgcat
     2221 gcgtcatcgc ggcctcgtgg tcgacgcgtt caaggcgacg ggcgtttccc tgcgccgcga
     2281 catcatggca ttcgaagacg ccggcgcgcg caatacgctc atattcacgg acttcggcat
     2341 cgtctccacc acgcgcaagg tgggccccgc gctcacgcgc accatgctca cggagctggc
     2401 ggagaagaaa cccgacgtca tcgtgttcga gctcggcgac ggcttgttag gcacctacgg
     2461 cgtcgattcc atcctcgagc aggaggacat ccgcaaggcg ttgaccggtg tcgtcctctc
     2521 ggccaacgat ccggtcgcgg cctacggcgg tgtcgatatc ctgcgcaagc ggttaaacat
     2581 cgagccggtc gcggtcacgg gcccggccac cgacaacgag gtcggagtgg cgatcatccg
     2641 cgatcagctc aaggtgccgg cgttcaacgc gctggcggcg ggcgccgcgc tgggcgactc
     2701 gatcatcgaa tccgtcggcc tcacgcaggc gcgtccgctc gatcgcgcgg caaacgaata
     2761 acgcgattcg atgaaccaaa tggccaacaa gattcctgcg attgtgctgg gcggcacggg
     2821 ctacgtgtcg ggtgagttgc tgcgcctgat cattggccac ccggacttca cgctcgcggg
     2881 tgtgatgtcc gacagctcac ccggtgagat cgcgggcaaa tcgtttccgc atcttgcggg
     2941 cgcgctcggc accaccgcgt tcaagtcgca ggcggaaatc gccgcgctga ttgcgaagga
     3001 gccgacgtcc gcggt
//
LOCUS       DASQJS010000028         4223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_331744, whole genome shotgun sequence.
ACCESSION   DASQJS010000028 DASQJS010000000
VERSION     DASQJS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331744"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VEW08_01255"
     CDS             <1..215
                     /locus_tag="VEW08_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39381.1"
                     /translation="SAGSSIRPQPVCCLARRSRRSARRNGLTIGVSRALADRLPNVPG
                     VPLKVTAGTIAGEQINLALVLGTPGP"
     gene            complement(239..712)
                     /locus_tag="VEW08_01260"
     CDS             complement(239..712)
                     /locus_tag="VEW08_01260"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ39382.1"
                     /translation="MHPRAAELIKQLNLERHPEGGYYREIFRSADLVAPADGRGPRAA
                     LTTIYYLLPAGIVSRWHRVASDEVWHLYEGGPLEVLSVDPAFSTLSRHRLEPLAPVCT
                     VAATHWQAARTLGDYALTGCTVGPGFDFEDFTILANDGASTARLLALDQELASLV"
     gene            complement(724..1566)
                     /gene="dapD"
                     /locus_tag="VEW08_01265"
     CDS             complement(724..1566)
                     /gene="dapD"
                     /locus_tag="VEW08_01265"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HYJ39383.1"
                     /translation="MTDQTTLAATIDEAFERRADINAKNSPPALRHAIEECIELLDSG
                     QARVAEKKSGQWTVNQWLKKAVLLYFRTHDNAVIDAGYTRFFDKVPLKYAARSQADEG
                     EALRAGGTRVVPPAVARRGSYIAPNTVLMPSYVNIGAYVDSGTMVDTWATVGSCAQIG
                     KNVHLSGGVGIGGVLEPLQASPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVFIGQS
                     TKIYDRATGQILQGRIPAGSVVVSGNLPSSDGKYSLYCAVIVKRVDAQTRAKTSINEL
                     LRAE"
     gene            complement(1567..2763)
                     /gene="dapC"
                     /locus_tag="VEW08_01270"
     CDS             complement(1567..2763)
                     /gene="dapC"
                     /locus_tag="VEW08_01270"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HYJ39384.1"
                     /translation="MNGRLELLAAYPFERLARLKSGITPPAELPHIAMSIGEPKHAPP
                     PFVIEALRRELSKLDSYPVTAGLPETRASFAAWLARRFSVNVSAENMVLPMNGTREAL
                     FSFVQAVVSTRGTQLKPVVAMPNPFYQIYEGAALLAGAEPVFLNTTAGNRFQPDLDSV
                     PAENWKRCEILFLCSPGNPTGAVLSLEFLRHALKLAEKYDFVIASDECYAELYRDENS
                     PPPSLLQAALAEGHDSFQRCMVFHSLSKRSSVPGLRSGFVAGDPALIKPYLLYRTYHG
                     CAMPVPTQLASIAAWNDDAHAAANRALYREKFARVLPILAPVLDVVEPDGAFYLWPDV
                     KCDDETFTRDLYATQNLTILPGSYLARDTRAGNPGRQRVRISLVATVEECVIAAQRIR
                     TFLEKT"
     gene            complement(2760..>4223)
                     /locus_tag="VEW08_01275"
     CDS             complement(2760..>4223)
                     /locus_tag="VEW08_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYJ39385.1"
                     /translation="EEFRQNPRNHRLFLEILRAPAGVTHELRRMNTYGVLGRYIPAFG
                     RIVGRMQYDLFHAYTVDAHTLFVLSNLRRLSLPKYDHELPQLSAIMQQLPKLEIAYLA
                     ALFHDIAKGRGGDHSQLGAVDAEAFCLEQGLSRYDARLVAWLVQNHLELSVTAQKQDI
                     SDPEVVNAFARKVGDETHLDYLYVLTSADVRGTNPKLWNSWKASLFQEFYERVKRALR
                     RGLESPIDPDELMRETQDGARKLLAASGRITAPEITRAWERLTAAYFLRHTPEEVAWH
                     TQLLADREPGSEDPLVAVQSSSERGTTAILAFAPHRYNSFAITTAVLDQLGLNIVDAR
                     ITPTGDGFSLDLYHVLEEDGAPIGDRERIGEIERALWRALQHPDEAPVTVTRRVPRQA
                     RMFRTSTQIAVTVDERNRRSVLELVSGDRPGLLCDVGKVLMEERVDLLAAKIMTVGER
                     AEDVFYLASLSGSPLDEAAAMRLQQRLSGALDRRAAA"
BASE COUNT          713 a         1304 c         1452 g          754 t
ORIGIN      
        1 catcggcggg cagttcgatc cgaccgcaac cggtgtgttg cctggcacgg cgttcacgcc
       61 gatccgctcg ccgtaacggg ctaactatcg gggtttcccg cgctctggcc gatcggttac
      121 cgaatgtgcc gggcgtgcca ctcaaggtga ccgcgggcac catcgcgggc gagcagatca
      181 acctcgcgct ggtgctcgga actccggggc cgtagcccca ctcatttggc aggccccatc
      241 agaccagcga cgcgagctcc tgatcgagag cgagcaggcg cgcggtgctg gcaccgtcat
      301 tggcgagaat cgtgaagtcc tcgaaatcga accccgggcc caccgtgcag cccgtcagcg
      361 cgtagtcgcc gagcgtgcgc gcggcctgcc agtgcgtcgc cgcgaccgta catactggtg
      421 cgagaggttc gagcctgtgg cgggaaagcg tgctgaacgc gggatccacc gacaacacct
      481 cgagcgggcc gccttcgtac agatgccaga cttcgtccga ggcgacgcgg tgccagcggc
      541 tgactatgcc ggccggtaaa aggtaataga tagttgtgag cgccgcgcgc ggcccacggc
      601 cgtccgccgg agcgaccaga tccgcagaac gaaagatctc acggtaataa ccgccctcgg
      661 gatggcgttc gaggttgagc tgcttgatga gctcggcggc gcgtgggtgc atggtggcgt
      721 tgctcactcg gcgcgcagca actcgttgat agacgtcttc gcgcgcgttt gcgcatcgac
      781 gcgcttgacg atgacggcgc agtacagcga gtacttgccg tcggacgagg gcaggttgcc
      841 cgacaccacc acggatccgg cggggatacg accctgcagg atctgccccg tggcgcggtc
      901 atagatcttg gtgctctggc cgatgaacac acccatcgaa atcaccgagc cttcttcgac
      961 gatgacgcct tcgaccactt ccgaacgcgc gccgatgaaa cagttgtctt cgatgatggt
     1021 gggcgaggcc tgcagcggct cgagcacgcc accgatcccc acgccgcccg agagatggac
     1081 gttcttgccg atctgcgcgc aggaaccgac cgtggcccag gtgtcgacca tggtgccgct
     1141 atctacatag gcgccgatgt tcacgtagct cggcatgagc accgtgttgg gcgcgatgta
     1201 cgagccgcgc cgcgcgacgg cgggcgggac cacgcgtgtg ccgcccgcgc gcagcgcttc
     1261 gccttcgtct gcttgcgagc gtgcggcgta cttgagcggc accttgtcga agaagcgcgt
     1321 gtatcccgcg tcgatcaccg cattgtcatg ggtgcggaaa tacagcagca ccgctttctt
     1381 gagccactgg ttcacggtcc attgaccact cttcttttcc gccacgcgcg cctggcccga
     1441 atccagcagc tcgatgcact cctcgatggc gtggcgcagt gccggcggtg aattcttcgc
     1501 attgatatcc gcgcgacgct cgaaggcctc gtcgatggtg gcggcgagag tggtctgatc
     1561 ggtcacttaa gtcttctcca ggaatgttcg gatgcgctgc gctgcgatca cgcattcttc
     1621 caccgtcgcg accagcgaga tgcgaacgcg ctgccgcccc gggttgcccg cgcgtgtatc
     1681 gcgcgcgaga tagctgccgg gcaggatggt gagattttgc gtggcataca agtcgcgcgt
     1741 gaaggtttcg tcgtcgcact tcacgtcggg ccacagatag aacgcgccgt ccggctccac
     1801 cacgtcgagt acgggcgcga gaatcggcag tacgcgcgcg aatttctcgc gatacagcgc
     1861 gcggttggcg gccgcgtgcg cgtcgtcgtt ccaggcggcg atgctcgcga gctgcgtcgg
     1921 caccggcata gcgcagccgt ggtaggtgcg atagagaaga tagggtttga tcaacgcggg
     1981 atcgccggcg acgaagccgg agcgcagccc gggaacgctg gatcgcttgg acagggaatg
     2041 gaacaccatg cagcgctgga aagaatcatg gccttcggcc agcgcggcct gcagcaggga
     2101 aggaggcgga gaattctcgt cgcggtacag ctcggcgtaa cattcgtcgg acgcgatgac
     2161 gaagtcgtac ttttccgcga gcttgagcgc atggcggagg aattcgagtg acaacaccgc
     2221 accggtcggg ttgccggggc tgcagaggaa gaggatttcg catctcttcc agttctcggc
     2281 gggcacggaa tcgaggtcgg gttgaaatcg attgccggcg gtcgtgttga ggaacaccgg
     2341 ttccgcgccg gcgagaaggg ccgcgccttc gtagatctgg tagaacggat tcggcatcgc
     2401 gaccacgggc ttcaactggg tcccgcgggt actgactacc gcctggacga acgagaacag
     2461 cgcctcgcgc gtgccattca tcggcagaac catgttctcc gccgacacat tcacgctgaa
     2521 tcttcgcgcc agccacgcgg cgaagctcgc gcgtgtctcg ggcagcccgg cggttaccgg
     2581 atagctgtcg agcttcgaga gctcgcggcg gagcgcttcg atgacgaacg gtggtggtgc
     2641 gtgtttgggc tcgccgatgg acatggcgat gtgcggcaac tcggccgggg gagtgatgcc
     2701 gctcttgagc cgcgccagtc gttcgaaagg atacgcggcg agtagttcga ggcgcccgtt
     2761 cacgcggcgg cgcggcgatc gagcgcgccg gagagccgct gctgcaggcg catggccgcg
     2821 gcttcgtcga gcggtgaccc ggacagcgac gccaggtaga acacgtcctc ggcgcgctcg
     2881 ccgaccgtca tgatcttggc ggcgagcagg tcgacgcgtt cttccatgag caccttgccg
     2941 acatcgcaca gcagtccggg gcggtcgccg gagacgagct cgagcaccga gcggcgattg
     3001 cgctcgtcga ccgtgaccgc aatctgcgtc gaggtgcgga acatgcgcgc ctggcgcggc
     3061 acgcgccgcg tgaccgtgac cggtgcttca tcgggatgct gcagggcgcg ccacaacgcg
     3121 cgttcgattt caccgatgcg ttcgcggtcg ccgatcggcg caccgtcttc ttcgagcacg
     3181 tggtagagat cgaggctgaa cccgtcgccc gtgggcgtga tcctggcgtc aacgatgttg
     3241 aggccaagct gatcgagcac cgccgtcgtg atggcgaagc tgttgtagcg atgcggcgcg
     3301 aacgcgagga tggcggtggt gccgcgctcg ctgctcgact gcacggccac cagcgggtct
     3361 tcgctgccgg gctcgcggtc ggctaacaac tgcgtgtgcc aggcgacttc ctcgggggtg
     3421 tgccgcagga aatacgcggc ggtcaggcgt tcccaggcgc gcgtgatttc cggggcggtg
     3481 atgcggccgc tggcggccag cagcttgcgc gcgccatcct gggtctcgcg catgagttca
     3541 tccggatcga tcgggctttc gagtccacgg cgcagcgcgc gcttcacgcg ttcgtagaat
     3601 tcctggaaca gcgatgcctt ccaggagttc cacagtttcg ggttggtgcc gcgcacgtcg
     3661 gcgctggtca atacatacag gtagtcgagg tgagtttcgt cgcccacttt tcgcgcgaag
     3721 gcgttcacga cttcgggatc cgagatgtcc tgcttctgcg ccgtcaccga cagctccagg
     3781 tggttctgca ccagccatgc gaccaggcgc gcgtcgtaac gcgacaggcc ctgttcgagg
     3841 cagaacgctt cggcgtccac cgcgcccaat tgcgagtggt caccaccacg gcccttggcg
     3901 atgtcgtgga agagtgcggc gaggtaggcg atctcgagct tcggcagctg ctgcatgatg
     3961 gccgagagct gcggcagctc atggtcgtac ttcggcagcg agaggcgtcg caggttgctc
     4021 agcacgaaca gcgtgtgggc atcgacggtg taggcgtgga acaggtcgta ctgcatgcgt
     4081 ccgacgatgc ggccgaacgc ggggatgtac cggccgagca cgccgtaggt attcatgcgg
     4141 cgcaactcat gggtcacgcc cgccggggct cgcaggatct cgaggaacag acgatggttg
     4201 cgcgggttct ggcggaactc ttc
//
LOCUS       DASQJS010000029         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_332012, whole genome shotgun sequence.
ACCESSION   DASQJS010000029 DASQJS010000000
VERSION     DASQJS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332012"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VEW08_01280"
     CDS             <1..237
                     /locus_tag="VEW08_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39386.1"
                     /translation="FVLGYPFALLLRKTGRLSALNVCAASFVIGFLVTAAAYLLDPSG
                     ALLILYPMIGGAMGLFAAIVFCLVAGIPFRRATP"
     gene            234..659
                     /locus_tag="VEW08_01285"
     CDS             234..659
                     /locus_tag="VEW08_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39387.1"
                     /translation="MMVGALLCIAGLIYGLLGALHALYTLLDMRDPRRIVPDDPAVIT
                     AMQNSKIRLTRGESTMWQGWVGFNLSHSLGALMFSAACFIVAACFRILAFSPWALFAL
                     AAVSALYLLMAVQYWFRIPILGTAVATASLAAAWLLYQL"
     gene            830..>2462
                     /gene="glmS"
                     /locus_tag="VEW08_01290"
     CDS             830..>2462
                     /gene="glmS"
                     /locus_tag="VEW08_01290"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HYJ39388.1"
                     /translation="MCGIVGAIAERNVVPILMEGLRRLEYRGYDSAGIAVLNGTAKLK
                     RLRTVGKVKMLEQAIEADPTHGQVGIAHTRWATHGVPSERNAHPHISHDGLAIVHNGI
                     IENHEELREDLKKAGYTFTSETDTEVIAHRVHFHLAAQKDLFKAVRATVAELEGAYAL
                     AVVSEEEPERIILAREGCPVVVGLGVGENFVASDVAALLPVTRRFMFLEEGDVADIHR
                     TSIRVLDKNGDTAERPVRESELSADAAEKGQYRHFMLKEIHEQPRAVADTLAERVANG
                     RLLEAAFGPAATQIFKHTEHVHIIACGTSYHSGVVARYFIEQICKLPCTVEIASEYRY
                     RNPVVPKNSLFVTISQSGETADTLAALRLAKQAGYLSSLAICNVPESSLVRESELVML
                     TRAGPEIGVASTKAFTTQLTALSMLVIALAKHRTADAERERSLVQRLIEIPSVIEKTL
                     ALDPIIYDLAKRFADKQHALFLGRGALHPIAMEGALKLKEISYIHAEAYPAGELKHGP
                     LALVDADMPVITVAPNNDLLEKLKSNLMEVRARGGE"
BASE COUNT          431 a          816 c          749 g          466 t
ORIGIN      
        1 ttcgtgctgg gctatccgtt cgcgctgctg ctcaggaaaa ccggcaggct ttcggcgctc
       61 aacgtttgcg ctgcctcgtt cgtcattggt ttcctggtga cggcggcggc gtacctcctt
      121 gatccctccg gtgctttact aatcctgtat cccatgatcg gcggagcgat gggcctcttc
      181 gcggccatcg tgttctgcct ggtcgccggc attcccttca ggcgcgcaac cccatgatgg
      241 tcggcgcgct gctgtgtatc gccggcctca tctacgggtt gttaggagcc ctgcacgcgc
      301 tgtatacctt gctcgacatg cgcgatccgc ggcgcatcgt gccggacgat ccggcggtca
      361 tcaccgccat gcagaattcg aagattcgcc tgacgcgtgg cgaatccacc atgtggcagg
      421 ggtgggtggg gttcaacctg agccacagcc tgggcgcact gatgttctcg gcggcctgct
      481 tcatcgtcgc cgcctgtttt cggattctgg ccttctcgcc gtgggcgctt ttcgcgctgg
      541 ccgcggtcag cgccctctat ctgctgatgg cggttcagta ctggtttcgc atcccgattc
      601 tcggtaccgc cgtcgccact gccagcctgg ccgccgcctg gctgctctac cagctctagg
      661 ccagattccc ggccctctta aggtgttgat ttctaatacc taaaggccat tccagcgacc
      721 gattgtgatc ggggtatcag aagtccctct aagggtgggt ttactactgc gttctgcgag
      781 ccgcgtcagg cacactagcg cccccctgca gccttgtgag cactgataaa tgtgcggaat
      841 agtcggagcc atcgctgagc gaaacgtcgt ccccatcctc atggaaggac tgcgccggct
      901 tgaataccgt ggctacgact ctgccggcat tgccgtactg aatggcaccg cgaagctcaa
      961 gcggcttcgc accgtcggca aggtcaagat gctcgagcag gccatcgagg cggatcccac
     1021 gcacgggcag gtcggcatcg cgcacacgcg ctgggccacg catggcgtgc cttccgaacg
     1081 caacgcgcac ccgcacatct cgcacgatgg cctggccatc gtccacaacg gcatcattga
     1141 aaatcacgag gagctgcgtg aggacctgaa gaaggccggt tacacgttca cctcggaaac
     1201 cgacaccgaa gtgatcgcgc atcgcgtgca ctttcatctc gccgcgcaaa aggacctgtt
     1261 caaggccgta cgggccaccg tggccgagct cgaaggcgcc tacgcgctgg cggtagttag
     1321 cgaagaggaa ccggagcgca tcatcctggc gcgtgaaggc tgccccgtgg tcgtcggcct
     1381 cggggtcggc gagaacttcg tggcctcgga cgtggcagcg ctgttgcccg tgacgcgccg
     1441 cttcatgttc ctggaagaag gcgacgtcgc cgatattcac cgtacctcga tccgggtgct
     1501 ggacaagaac ggcgacaccg ccgaacgtcc cgtgcgcgag agcgagctgt cggccgacgc
     1561 ggccgagaaa ggccagtacc gccacttcat gctcaaggaa atccacgagc agccgcgcgc
     1621 cgtcgccgac acgctggccg aacgcgtggc caatggccgg ttgctggaag ctgcgttcgg
     1681 ccccgcggcc acgcagatct tcaagcacac cgaacacgtg cacatcatcg cgtgcggcac
     1741 cagctaccac tccggtgtcg tggcccgtta cttcatcgag cagatctgca aactgccgtg
     1801 caccgtcgag atcgcgagcg aatatcgcta ccgcaatccc gtggtgccca agaattcgct
     1861 gttcgtcacg atttcgcagt cgggagagac ggccgacacg ctggctgcgc tgcggctcgc
     1921 gaaacaggct ggttatctgt cgtcgctggc catctgcaac gttcccgaga gctcgctggt
     1981 gcgcgaatcg gagctggtca tgctcactcg tgccggcccc gagatcggcg tggcatccac
     2041 caaggcgttc accacgcagc tgacggcgct cagcatgctg gtcatcgcgc tggccaagca
     2101 ccgcaccgcc gacgccgagc gcgagcgcag cctcgtccag cgactcatcg agattccttc
     2161 ggtcatcgag aagacgctgg cgctggatcc gatcatctac gacctggcca agcgcttcgc
     2221 cgacaagcag cacgcgctgt tcctcgggcg cggcgccctg caccccatcg cgatggaagg
     2281 cgccctcaag ctcaaggaaa tttcctatat ccacgccgag gcgtatcccg ccggcgagct
     2341 caagcacggg ccgctggcgc tcgtggacgc ggacatgccc gtcatcaccg tggcgcctaa
     2401 caatgacctg ctcgagaagc tcaagagcaa tctcatggaa gtgcgggcgc gcggcggcga
     2461 gc
//
LOCUS       DASQJS010000030         7857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2531309, whole genome shotgun sequence.
ACCESSION   DASQJS010000030 DASQJS010000000
VERSION     DASQJS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2531309"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VEW08_01295"
     CDS             <1..528
                     /locus_tag="VEW08_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39389.1"
                     /translation="AHHYFRNVIRPGKKSKEKVDVFSFELLAYRELINPRAMPNSTVP
                     AEQLPGYFTTADDITPREHVDIQAAAQRWVDSSISKTANVPTDYKYESFKDIYLYAHE
                     KDLKGCTTFRFNPEAFQGVLVKEDDLKNTTYVFTLEDGSEISVKGDEEIEYDGEKHTA
                     ANLYDALKEGYYGKF"
     gene            542..1315
                     /locus_tag="VEW08_01300"
     CDS             542..1315
                     /locus_tag="VEW08_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39390.1"
                     /translation="MHKIDKKIAKYRVQKAEDKAAADAAAGAAATAVATAVAAAVAKF
                     EPAVEMTHDKQGRSSKVVRMTEEVQRPEVLVGSTYKIKTPVSDHAMYVTINDIILNEG
                     TPYEQRRPFEVFINSKNLDHFQWIVALTRIISAVFRKGGDVTFLVDELKAVFDPRGGY
                     WQAGGKFMPSIIAELGYVIEKHLQTIGLLKKVELDDNEKKLIAEKKAEFEERTKQTDA
                     FAKSHFPDGAQLCAKCSTAAVVMMDGCMTCLNCGDSKCG"
     gene            1605..1994
                     /locus_tag="VEW08_01305"
     CDS             1605..1994
                     /locus_tag="VEW08_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39391.1"
                     /translation="MKLGAIFAAFVSSLMLAAMAFADASPTANKWRIEMSGQALTTGE
                     ILFRVTPRQGEAVDVSVGIRSGRAENNVARDVRDALAAKLSPDRYTIEVDDGEDILIK
                     KKDGQPDFALELVESNVQNVSIKVEGE"
     gene            2074..2658
                     /locus_tag="VEW08_01310"
     CDS             2074..2658
                     /locus_tag="VEW08_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39392.1"
                     /translation="MNRATCLIAALVAASALAAPSSMAQGPPLASAWNIAASGSATSS
                     GDLDFRITPNDGSNPVDITVPVISGAGQDSVARSIGRALSSQLRSDRYNVQLGENGNV
                     LVTDPRGQPNFSLELVDSNIESVRVAVQIVTPAAPPTAPMQAAPAEAPPAIRPDNTVP
                     GNAAPPPANAPPSPNPAPDANEAAGAPSSPPPPR"
     gene            3070..3363
                     /locus_tag="VEW08_01315"
     CDS             3070..3363
                     /locus_tag="VEW08_01315"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HYJ39393.1"
                     /translation="METVYETTLAFHAQMICDLLISAGVPARVDGSYLQSIGGEIPLG
                     NAVRVRVETARAAEARAIIAEWESAEIPSDEEAAAEAEAAGLGAGKLPADGQQ"
     gene            complement(3424..5205)
                     /locus_tag="VEW08_01320"
     CDS             complement(3424..5205)
                     /locus_tag="VEW08_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HYJ39394.1"
                     /translation="MSNVLRALRRASILCTLLLLPVASFAGFFISNGQLVDNNGTPFI
                     MRGVNYPYTWFQSRNTQQDLAAIAATGSNTVRIVLSTGGQWARVNGTQVAQLIQWCKD
                     LRMIAVLEVHDSTGWSEQTTAVPISNATAYWLSSDIRAAINGQENFVIINIANEPFGN
                     NTSANYTPDTITAIQALRSAGLTHNIMIDAANWGQDWSNTMRTNAMALWNADTLRNLT
                     FSVHMYEVYQSLSTVSTYMQGFDDMNLPLVIGEFGPINNGQFSDSDSVIAQAQARGNG
                     YLGWSWSGNGGGGTGLDMTVNFDPAQLTTWGNRIVNGTNGIRATSVLATVFGTPTNNL
                     VVAPTTLSFASAASSSPVTVTANVSWTITDNQTWITASPASGTNNGSFTVSATANTGT
                     ASRTGTVTVTGGGLTRTIAVTQAAPTANNLTLSQTSLSFASAASSSAVNVTANVSWTV
                     TDDQTWLSASPTSGSGNGSVTLNATANTATTSRTGTVTVSGGGITRTVSVTQAGQSGG
                     GTGGTCPNPVTFSGNTGNFNTTGAACYRTNVNINGWGCYNFDGRTVTVGGVARTCGQM
                     PLTRAADGYYYFAVTAGQYPWAGLYAW"
     gene            5497..6843
                     /locus_tag="VEW08_01325"
     CDS             5497..6843
                     /locus_tag="VEW08_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39395.1"
                     /translation="MTTTVRILGSRAREQVPGTGITLLAPGFRGALTQLAAGASPIAE
                     HDLPFADAAAGAEVMLAAQLTLSIEARPAEGVGLRSRSAVAAHPRVIVPRRSGVAYAL
                     LQTDETGASSFVLPSQRVQSEVVFPLTISAHGATRRTLRVLMWPAQPVTDIGALAIAA
                     RWEQLRRPSQLAQCGPDGDWLEPDWPALAAGPVLLLLHDTFSTPQATFADWIGDTSFA
                     SVLHGFGGRCLAYAHPTLSSGLEENVSWLWSQIAHLPGPFDIVALGRGGLLARAIAAD
                     GRLPLRRVCQVGVPNNGTSLALDASLRRFLDGHVAMLACIPSRVARTTLEGVLCLARV
                     AAMGLASPLPGIAIQAPGNPVLAALHAPSQSDQQWFTVSAQFARAGGHGNWPVDANDF
                     AAIPNDLVVPSEGCHEPGTPVTDRLRLGGSLVHHHNYFANAHVRAHLAQWFAAERL"
     gene            6972..7190
                     /locus_tag="VEW08_01330"
     CDS             6972..7190
                     /locus_tag="VEW08_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39396.1"
                     /translation="MSSTKIIAIVLIVAGVLGLAYGKFSYTKETHEAKVGPLEMSVKD
                     KETVNIPVWAGVGAIVIGAGLLLVGKRT"
     gene            7473..>7857
                     /locus_tag="VEW08_01335"
     CDS             7473..>7857
                     /locus_tag="VEW08_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HYJ39397.1"
                     /translation="MKRNADISLGRRQRGFTLMELMAAIAVLGVLSAVAVPSFTNLIN
                     RNRLTSQANELLSSIQFARIEAIRSNARVTFCGAASATADGEDDCVDGAQPFWVVIGG
                     DQQLRVFAVKDPMQISTDLEKISFYA"
BASE COUNT         1457 a         2465 c         2483 g         1452 t
ORIGIN      
        1 gcacatcact atttccgcaa cgtgattcgc cccggcaaga agagcaagga gaaagttgat
       61 gtcttctcat tcgagcttct cgcctatcgc gaactcatca atccgcgtgc gatgccgaac
      121 tcaacggtcc cggccgaaca gttgcctggc tatttcacga cggcagatga catcacgccg
      181 cgagaacatg tcgacatcca ggcggcggcg cagaggtggg tcgacagctc catttcaaag
      241 actgcgaacg tgcctacgga ttacaaatac gagagcttca aagacatcta tctgtacgcc
      301 catgagaagg atctgaaggg ctgcaccacg ttccgcttca acccggaagc attccagggc
      361 gtgctggtga aggaagacga cctcaagaac accacctacg tgttcacgct cgaggacggt
      421 tcggagattt cggtgaaggg cgacgaagag atcgaatacg acggtgagaa acacaccgcc
      481 gcgaatctct acgacgcatt gaaagaaggc tattacggca agttctagcc ggagaccact
      541 catgcacaag atcgacaaga aaatcgcgaa gtaccgcgtg cagaaggccg aggacaaggc
      601 tgccgcggac gcggccgccg gcgccgcggc caccgcagtc gccaccgcgg tggctgccgc
      661 ggttgccaag ttcgagcccg ccgtcgagat gacgcacgac aagcagggcc gctcctcgaa
      721 ggtcgtgcgc atgaccgaag aggtccagcg ccccgaggtg ctggtcggct cgacgtacaa
      781 gatcaagacg ccggtgtccg atcacgcgat gtacgtgacg atcaacgaca tcatcctcaa
      841 cgagggcacg ccctacgagc agcgccgccc gttcgaggtg ttcatcaatt cgaagaacct
      901 cgatcacttc caatggatcg tggcgctcac gcgcatcatc tccgcagtgt tccgcaaggg
      961 cggcgatgtc acgttcctgg tcgatgagtt gaaagccgtg ttcgatccgc gcgggggtta
     1021 ctggcaggcg ggcggcaagt tcatgccgtc gatcatcgcc gagctgggct atgtcatcga
     1081 gaagcacttg cagaccattg gcctgctcaa gaaggtggaa ctcgatgaca atgaaaagaa
     1141 gctgattgcc gagaagaaag cagagttcga agaacgcacc aagcagaccg acgcattcgc
     1201 caagtcgcat tttccggacg gcgcgcagct gtgcgccaag tgcagcaccg ccgccgtcgt
     1261 catgatggac ggctgcatga cctgcctgaa ctgcggcgac tcgaagtgcg ggtgatttcg
     1321 atttccgggg agggttaact cctttcgcgt gtcgcggccg ggttccccgc ccggctcctg
     1381 tctcgatgga ctaacctggc aggaccagcg attcgcgatt caacaaggca gcttcgaggc
     1441 tgccttgttt ttgtgtgcgt gtcggccgac gcctccgcgc gaccgagcgg tgctgaaggc
     1501 actacttcca gatcgccgac aggcgttcgg gtgcccgtcg gatgccacgc gcccgcaccg
     1561 acgaggagac tcacattcac agaaattgaa atgtggggtt cttcatgaag ctcggtgcca
     1621 ttttcgctgc cttcgtttcc tcgctgatgc tcgccgccat ggcgttcgcc gatgcatcgc
     1681 ccaccgccaa caagtggcgc atcgaaatga gcgggcaggc gctgaccacg ggagaaatcc
     1741 tgtttcgcgt gacgccgcgc cagggtgagg ccgttgacgt gagcgtcggc atcaggagcg
     1801 gccgcgccga gaacaacgtg gccagggacg tgcgcgatgc gctcgccgcc aagctcagcc
     1861 ccgatcgtta caccatcgaa gtcgacgacg gcgaggacat cctgatcaag aagaaggatg
     1921 gccaaccgga ttttgcgctc gagctcgtcg agtcgaatgt acaaaacgtc agtatcaagg
     1981 tcgaaggcga atagccgtca gccgaaagtc agtgcgcccc gggatctgcc ggggcgcgcc
     2041 tgcccgggag tcagcgcagg caaggagtca gtcatgaatc gcgctacctg cctcatcgcc
     2101 gccctcgtgg cggcgtctgc attggcggcc ccatcttcaa tggcccaggg tccgccattg
     2161 gccagcgcct ggaacatcgc ggccagcggc agcgccacct cgagtggcga cctcgacttc
     2221 cgtatcacgc ccaacgatgg cagcaacccg gtggatatca ccgtgccggt gatttccggc
     2281 gccggacagg attcggtggc gcgcagcatc ggccgggcgc tcagctcgca gttgcgcagc
     2341 gatcgctaca acgtgcagct cggcgaaaac gggaacgtgc tggtcaccga cccgcgcggc
     2401 cagcctaact tctcgctcga gctggtcgac tcgaacatcg agagcgtgcg cgttgccgtg
     2461 cagatagtga cgccagcggc accaccgact gcgccgatgc aggcggcgcc ggccgaggcg
     2521 ccgcctgcga tccggccgga caacacggtg ccgggaaatg ccgcgccacc accggcgaac
     2581 gctccgccat cgccgaatcc ggcacccgat gccaacgagg cggcgggggc tccgtcgagc
     2641 ccaccgccac cgcgctgatc agttatctgt caccagccga agcactgagc gttttgggcg
     2701 aacacgccca gaccggatgc gtcgtagttg aagctgccga cttttcggtc agcgagtact
     2761 cctggcagaa gcccgtgagc tcgaaggcga agtcgccgac gaacaaccca tacaacgaga
     2821 atgcccgagc catcggaacc tgcccgagca tgcgcaggct cagcgagcgc agttcatcgg
     2881 tgatctggtc gggatactcg atgtggcgtg agtcgtcgta cttgcacgcc tgccactgca
     2941 gtttcggggg ctctaaccac cgcctgaaag cagggtcaac gacccgaata cgcttgccat
     3001 gagccggaat tctgcgacac gacccggcag ctatttttga gccgcaaggg cgtgtacctt
     3061 gattccacca tggaaacggt ctacgaaacg actctcgcct ttcacgcaca gatgatctgc
     3121 gacctgctca tctccgcggg cgtgcctgcc cgggtggatg gctcatattt gcagagcatt
     3181 ggcggcgaga ttccgctggg caatgccgtg cgcgtgcgcg tcgagaccgc gcgtgccgcc
     3241 gaagcgcgcg ccatcattgc cgagtgggag agcgcggaaa taccgagcga cgaggaagcg
     3301 gcggcggaag cggaagcagc cggcctcggt gccggcaagc tgcccgcgga tggccagcaa
     3361 taaaagggcc actccgagga gtggccctgt atcgcgcagc atccgtaacg gctgctgatc
     3421 aaactaccag gcgtacaaac ccgcccacgg atactgtccc gcggtcactg cgaagtagta
     3481 atacccgtcc gccgcccgcg tgagcggcat ctggccgcaa gtgcgcgcga cgccacccac
     3541 tgtcaccgtg cgaccgtcga agttgtagca gccccagccg ttgatgttga cgttggtgcg
     3601 ataacacgcc gcgcccgtgg tgttgaagtt gccggtgttg ccgctgaacg tgaccgggtt
     3661 ggggcaggtg ccgccggtgc ctcccccgga ttgcccggcc tgcgttaccg agaccgtgcg
     3721 cgtgatgcca ccaccactca ccgtcacggt tcccgtacgg ctggtcgtgg cggtgttggc
     3781 cgtggcgttc aaggtgacgc tgccattgcc ggaaccactc gtcggtgaag cgctgagcca
     3841 ggtctgatcg tcggtcaccg tccagctgac attcgccgtg acattgaccg ccgacgagga
     3901 cgccgcggag gcgaacgaga gcgacgtctg cgacagagtg aggttgttag ccgtaggcgc
     3961 tgcctgggtt actgcgatcg tgcgcgtgag gccgccaccg gtgacggtga ccgtgcccgt
     4021 acggctggcc gtgccggtgt ttgccgtcgc gctcaccgtg aagctgccgt tgttggtgcc
     4081 gctcgccggc gaggcagtga tccacgtctg gttgtcggtg atcgtccagc tgacattcgc
     4141 agtgaccgtg accggtgatg acgaagctgc agaggcgaac gacagcgtcg tcggcgccac
     4201 cacgagattg ttggtcggcg tgccgaacac ggtggccagc accgaggtcg cgcgaatgcc
     4261 gttcgtaccg ttcacgatac ggttgcccca ggtcgtgagc tgcgccggat cgaaattgac
     4321 cgtcatgtcg aggccggtgc cgccgccgcc attaccggac caggaccagc cgaggtagcc
     4381 gttgccgcgt gcctgcgcct gtgcgatgac ggaatcggag tcggagaact gcccgttgtt
     4441 gatcgggccg aactcgccga tcaccagcgg caggttcatg tcgtcgaagc cctgcatgta
     4501 cgtgctgacc gtcgagagcg actggtacac ctcgtacatg tgcacgctga aggtcaggtt
     4561 gcgcagcgtg tccgcgttcc aaagcgccat cgcattggtg cgcatggtgt tcgaccagtc
     4621 ctgaccccag ttcgctgcat cgatcatgat gttgtgcgtg aggccggcgc tgcgcagcgc
     4681 ctggatcgcg gtgatggtgt cgggcgtgta gttagcggac gtattgttac cgaagggctc
     4741 gttcgcgatg ttgatgatca cgaagttttc ctgcccgttg atcgcggcgc ggatatcgga
     4801 gctgagccag tatgccgttg cgttcgagat cggcacagcc gtggtctgct cgctccatcc
     4861 cgtggagtcg tggacctcga gcacagcgat cattcgcagg tccttgcacc actggatgag
     4921 ctgtgcgacc tgcgtgccat tcacgcgtgc ccactggccg ccggtcgaca gcacgatgcg
     4981 cacggtgttg gaacccgtgg cggcgatggc cgcgagatcc tgttgcgtgt tgcgtgactg
     5041 gaaccacgta tatggatagt tgacgccacg catgatgaaa ggcgtgccgt tgttgtcgac
     5101 gagttgtccg ttcgagatga agaagccggc gaaactcgct accggcagta gtaagagagt
     5161 gcaaaggatt gaggcgcgcc gaagcgcgcg tagaacattg ctcatgactt ccccctgaag
     5221 ttccgtgtct ttgcggccgc atccatacga tctgagccgt gcgccgcgat gatatccctc
     5281 tcaaccgggc ttaatgcaag caaaaatagt tattgtcccg agattcgtcc cgatgtccgg
     5341 ttcgttgtcc ggaattggtt gcgctaactg caggaagagt tttcacgttg cgtgcaggag
     5401 gggctttcct agtcctttgt ccttgcgcgt gcgagcgcgc gtcgggaatt ttccacaact
     5461 atctactcgc atctcgttgc actactctcg atcgcgatga cgaccacggt gcgaatcctg
     5521 gggtcacgcg cccgcgaaca ggtgcccggc acgggcatca cgctgcttgc acccggattc
     5581 cgcggcgcac tcacgcaact cgctgccggc gcctctccca tcgccgagca cgacttgccg
     5641 tttgctgatg ccgccgccgg cgcggaggtc atgctcgccg cgcagctcac gctgagcatc
     5701 gaagcgcgtc ccgccgaagg cgtggggctg cgctcgcgca gcgcggtagc agcgcatccg
     5761 cgcgtcatcg ttccccggcg cagcggagtt gcctacgcgc tgctgcagac cgatgagacg
     5821 ggggcatcga gcttcgtgct tccctcgcag cgagttcaga gcgaggtggt attccccctg
     5881 actatctccg cgcacggcgc gactcgccgt acgctgcgcg tgctgatgtg gccagcccag
     5941 ccggtgaccg acatcggcgc gctcgcgatt gcggcgcgtt gggagcagtt gcgacgaccg
     6001 agccaactcg cgcaatgcgg tccggacggc gactggctcg agcccgactg gcccgcgctt
     6061 gccgctggac ccgtgctcct gctgctgcac gacaccttct ccacgccgca ggcgacgttc
     6121 gccgattgga tcggcgatac ctccttcgcc tcggtcctgc atggcttcgg cggccgatgc
     6181 ctggcgtacg cgcatccgac gctgtccagc ggcctggaag aaaatgtttc ctggctatgg
     6241 tctcagatcg cccacctgcc cggacccttc gacatcgtgg cgttgggccg tggcggcttg
     6301 ttagcgcgcg cgattgccgc cgatggaagg ctgccgctgc gacgcgtgtg ccaggtgggt
     6361 gttcccaaca acggcacgtc gctcgcactc gacgccagcc tgcggcgatt tctcgacggg
     6421 cacgtggcca tgctggcgtg cattcccagc cgcgtggcgc gcaccacgct cgagggtgtg
     6481 ttgtgcctgg cgcgtgtcgc ggcgatgggc cttgcgtcgc cgctgcctgg catcgcgatt
     6541 caggcgcccg gcaatccggt gctcgcggcg ctgcatgctc cctcgcaatc cgaccagcaa
     6601 tggttcacgg tgagtgcgca gttcgcgcga gccggcggtc acggcaattg gcccgtcgac
     6661 gcgaatgatt ttgccgcgat tcccaatgat ctggtggtgc cgagtgaggg ttgccacgag
     6721 ccaggcacac cggtcacgga tcgcctgcgt ctcggcggtt cgctggtgca ccatcacaac
     6781 tacttcgcta acgcgcacgt gcgcgcgcat ctggcgcaat ggtttgctgc cgaacgcttg
     6841 taggactccg ctgaacatca cacactccgc gcgacgatgg ttgcgcggat cggcttggcg
     6901 caatcttcga cacagtcgga ggcggcctac agccggcccg actcccgaag tcactacagg
     6961 gcatagataa aatgagttcc accaagatca tcgccatcgt tctgatcgtc gccggcgtgc
     7021 tgggcctggc ctatggcaag ttcagttaca ccaaggaaac gcacgaggcg aaggtggggc
     7081 ccctcgagat gtcggtgaaa gacaaagaaa cggtgaacat tcccgtctgg gccggtgtcg
     7141 gcgccatcgt gatcggcgcc ggcctgctgc tcgtcggtaa gcgcacctga tgatttgacc
     7201 cgtggggcgg tcccgccttg tggtgcgggg ccgctcccga ttgccggatc ccggcagcag
     7261 gtccctcgcc tcgagcgaat tcttttgtca aattgccgtc cgctggacgg catttttcgt
     7321 tgtgttttcc gctgcctcca gccgctcgta ggccgacaac gaccgctcgt cagggggccc
     7381 ccgcccccgg ggccgaagtg tggcattcat cacaaatcgg gcaatgcctc gcccctaaca
     7441 tcgacatcgc aatgaacagc aggtcacacg aaatgaaacg caacgccgat atctcgctcg
     7501 ggcgcaggca gcgcggcttc acgctcatgg agctgatggc cgcaatcgcc gtcctcggcg
     7561 tcttgtccgc cgtggcagta ccttccttta caaacctgat caacagaaac cgcctgacgt
     7621 cgcaggcgaa tgaactgttg tcctccatcc agttcgcgcg catagaagcc atccgcagca
     7681 atgccagggt cactttttgc ggcgcggcca gtgccaccgc ggatggtgaa gatgattgcg
     7741 tcgatggcgc gcagcctttc tgggtagtga tcggcgggga ccaacaactg cgcgtgttcg
     7801 cggtgaaaga ccccatgcag atcagcactg atctggaaaa aatcagcttc tacgcgg
//
LOCUS       DASQJS010000031         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2219550, whole genome shotgun sequence.
ACCESSION   DASQJS010000031 DASQJS010000000
VERSION     DASQJS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219550"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VEW08_01340"
     CDS             <1..989
                     /locus_tag="VEW08_01340"
                     /inference="COORDINATES: protein motif:HMM:NF019120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39398.1"
                     /translation="PPLFGAMPKTPLMMEFQITKEYLGFATHLAYLGTLYEEALDSDT
                     MVKGKGSTVAKVVDGELHGYRKTGMAGVSNIGADRNWTGSDFDQANWYVFGRLAWDPD
                     LSAQSIAQEWVRMTFSNDPAFVTPVVAMMMGSREAVVDYMMPLGLHHLFDTGHHYGPG
                     PWVDNLNRADWNPTYYHRADANGVGFDRGPRGSNAVAQYSPKVAAIFADRRKIPEHFL
                     LWFHHVPWDYKTSSGRILWDELVYRYTHGVEVVSQMRRTWAGLGKYVDAERFAQVSAF
                     LSIQEQEAQWWRDACIAYFQTFSKRPLPAGFAPPAHPLEYYRQLKFPYAPGR"
     gene            1012..2331
                     /locus_tag="VEW08_01345"
     CDS             1012..2331
                     /locus_tag="VEW08_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39399.1"
                     /translation="MRTVLLVCGIMTVAVAAFAGDLPPPLELTAEQDHKMMMERLGIS
                     SIRTGANGRDPNAPNAANYDEAKGNPYPDLPDALKMDSGKPVQNAKQWWNKRRPQLVE
                     HFDREVYGRMPKHVPGVSWAVAETRQETKSGIEVVTRKLLGHVDNSAYPLINVDIELH
                     LTTPVGAKGVPVIIEFFPPEWAARIPPQPSPTWQEQVIARGWAYAILSPTSIQADNGA
                     GLTRGIIGLVNKGQARKPDDWGALRAWAWGASRALDYLATDPAVDAKRAVIEGVSRYG
                     KASLVTMAYEPRFAIGFIGSSGMGGAALHRRRIGELLENVAGSGEYHWMAGNYIKYAG
                     PLTAKDLPVDSHELIALAAPRPLFIGAGSPEKGDAWVDPRGMFMATVAAGPVYRLLGK
                     RALDTTEFPPIETPLTAGELAYRQHAGGHTSYPNWPTFLDFASRYFD"
     gene            complement(2332..>3062)
                     /locus_tag="VEW08_01350"
     CDS             complement(2332..>3062)
                     /locus_tag="VEW08_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HYJ39400.1"
                     /translation="ISNLKTAYEPAESELPAETVWTTPKYLAVVPKLFERLRSEHGPD
                     IELLHDVHHRLSPIEAARLARDLEPYRLFWLEDATPAENQKSFELIRRHSVTPLAVGE
                     VFNSIWDCKHLIENQLIDYIRTTIVHGGGITHVRRIADFAALHGVRTGFHGATDLSPV
                     CMGAALHFDTWVPNFGIQEYMKHAPETDAVFPHDYTFREGRLFCGESAGHGVTIDEKL
                     AAKYPYSPKQLPIARLEDGTMWDW"
BASE COUNT          552 a          959 c          992 g          559 t
ORIGIN      
        1 tccctccgtt gtttggcgcc atgccgaaga cacctttgat gatggagttc cagatcacca
       61 aggagtacct cggcttcgcc acgcacctgg cgtatctcgg cacgctatat gaagaggcgc
      121 tggactcgga cacgatggtg aaaggcaaag ggtcgacggt ggcgaaggtc gtcgacggcg
      181 aattgcatgg ttaccggaag accggcatgg ctggtgtgtc gaacatcggc gctgatcgca
      241 actggaccgg atccgatttc gaccaggcga actggtacgt cttcgggcgt ctggcctggg
      301 atcctgacct gtctgcgcag agcatcgccc aggaatgggt gcgcatgacc ttcagcaacg
      361 acccggcgtt cgtcacgccc gtggtcgcca tgatgatggg atcgcgcgag gcggtggtgg
      421 attacatgat gccgctcggc ctccatcatc tctttgacac cggccatcac tatggcccgg
      481 ggccgtgggt cgacaatctg aatcgcgccg actggaatcc cacttactac catcgcgccg
      541 atgccaatgg ggtcggcttc gaccgcggac ccaggggcag caacgccgtc gcgcaataca
      601 gcccgaaggt cgccgccatc tttgccgacc ggcgcaagat cccggaacat ttcctgctct
      661 ggtttcatca tgttccctgg gactacaaga cctcatcggg tcgcatcctg tgggatgagc
      721 tggtgtaccg ctatacccat ggcgtcgaag tagttagcca gatgcgccgc acgtgggccg
      781 gcctcgggaa atacgtcgat gcggagcgtt tcgcgcaggt cagcgcgttc ctgtccatcc
      841 aggagcagga ggcgcagtgg tggcgcgacg cctgcatcgc ctatttccag acattctcga
      901 aacggccgct gccggcgggg tttgcgccgc ctgcgcatcc actcgagtac taccggcaac
      961 tcaagtttcc atacgcaccc ggccgctgag cgggtattcg agggggatgt gatgcgtacc
     1021 gttctgctgg tttgcggaat catgaccgtg gccgttgccg ccttcgcggg cgacttgcca
     1081 ccgccgctcg agctcaccgc cgagcaggat cacaagatga tgatggagcg ccttggcatc
     1141 tcgtccatcc ggacgggagc caatggccgc gacccgaatg cgccgaacgc ggctaactac
     1201 gatgaggcca agggcaatcc ctatccagac ctgcccgacg cgctgaagat ggacagcggc
     1261 aagccggtac agaatgcgaa gcaatggtgg aacaagcggc ggccgcagct cgtcgagcat
     1321 ttcgatcgtg aggtgtacgg caggatgcca aagcacgtgc cgggtgttag ctgggcggtc
     1381 gccgaaacca ggcaggagac caaaagcggc atcgaggtcg tcacgcgcaa gctgctcggc
     1441 cacgtcgaca attccgccta tccgctcatc aacgtcgaca tcgagctcca cctgactact
     1501 ccggtgggcg cgaaaggcgt gcccgtcatc atcgaattct tcccgcccga atgggcggcg
     1561 cgcatcccgc ctcagccgtc gccgacctgg caggagcagg tgattgcgcg cggctgggca
     1621 tacgccatcc tgtcgccgac cagcatccag gccgacaacg gcgctggcct cacgcgcggc
     1681 atcatcggcc tcgtgaacaa ggggcaggcg cgcaagcctg atgactgggg cgcgttgcgc
     1741 gcctgggcct ggggcgcctc gcgcgcgctc gactacctgg cgaccgaccc ggccgtggac
     1801 gcgaagcgcg ccgtaatcga aggggtgtcg cgttacggca aggcctcgct ggtgacgatg
     1861 gcgtacgagc cgcgcttcgc gatcggtttc atcgggtcct cgggaatggg tggcgccgcg
     1921 ctgcatcgcc ggcgcatcgg cgaattgctc gagaacgttg cgggcagtgg cgagtaccac
     1981 tggatggccg gcaactacat caagtacgca gggccgctca ccgcaaagga tctgccggtc
     2041 gactcacacg agctgattgc gctcgcggcg ccgcgcccgc tgttcatcgg cgcgggcagc
     2101 cccgagaaag gcgacgcctg ggtcgacccg cgcggcatgt tcatggccac ggtggcggcg
     2161 gggccggtgt atcgattgtt aggcaagcga gcgctcgaca ccacggagtt cccacccatc
     2221 gaaactccgt tgaccgccgg cgagctcgcg taccgccagc acgcgggcgg tcacacgtcc
     2281 tatccgaact ggccgacgtt cctcgacttc gcgtccaggt acttcgacta gctaccaatc
     2341 ccacatcgtg ccgtcctcga gacgggcgat gggcagctgc ttcggggagt acggatactt
     2401 cgcggcaagt ttttcgtcga tggtcacgcc gtggcccgcc gactcgccgc agaacaggcg
     2461 tccttccctg aacgtgtagt cgtgcggaaa cactgcgtcg gtttcgggcg cgtgcttcat
     2521 gtattcctgg atgccgaagt tcgggaccca ggtatcgaag tgcagcgcgg cgcccatgca
     2581 caccggtgac aggtcggtgg caccgtggaa cccggtacgc acgccgtgta acgctgcgaa
     2641 gtccgcgatg cggcgcacat gcgtgatgcc accgccatgc acgatggtgg tgcggatgta
     2701 gtcgatgagc tggttctcga tgaggtgctt gcagtcccag atggaattga atacttcgcc
     2761 aaccgccagc ggcgtcaccg aatgcctgcg aatcaactcg aagcttttct gattctcggc
     2821 gggtgtggcg tcttcgagcc agaacagccg ataaggctcg agatcgcgcg cgagccgcgc
     2881 cgcttcgatg ggcgacaagc ggtggtgcac gtcgtgcagc agttcgatgt cggggccgtg
     2941 ttcacttcgc aaacgttcga acagtttcgg caccacagcc aggtatttcg gcgtcgtcca
     3001 cacggtttcc gcgggcagtt cgctctcggc cggctcgtac gcggtcttga ggttcgagat
     3061 gc
//
LOCUS       DASQJS010000032         3381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_647957, whole genome shotgun sequence.
ACCESSION   DASQJS010000032 DASQJS010000000
VERSION     DASQJS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647957"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /gene="rplR"
                     /locus_tag="VEW08_01355"
     CDS             <1..346
                     /gene="rplR"
                     /locus_tag="VEW08_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HYJ39401.1"
                     /translation="KERRQRRAIKTRMKIRELGIARLTVHRTPQHIYAQVFDSQGAKV
                     IATASTVQEKVAKGLKGTGNVEAAKAVGAAIAEAAKAAGVTKVAFDRAGFMYHGRVKA
                     LADAAREAGLEF"
     gene            360..869
                     /gene="rpsE"
                     /locus_tag="VEW08_01360"
     CDS             360..869
                     /gene="rpsE"
                     /locus_tag="VEW08_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HYJ39402.1"
                     /translation="MARPDNKSQAQDEFLEKLVAVNRTAKVVKGGRQFGFTALTVVGD
                     GAGRVGFGFGKAREVPVAISKAMAQARKNMVNVALKNDTLHFAIKGAHGATMVYMQPA
                     SDGTGVIAGGGMRAVLECAGVRNVLAKSYGSRNPINVVRATVNALANVNSPETIAAKR
                     GKTLDEILG"
     gene            903..1085
                     /gene="rpmD"
                     /locus_tag="VEW08_01365"
     CDS             903..1085
                     /gene="rpmD"
                     /locus_tag="VEW08_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HYJ39403.1"
                     /translation="MANKQIKVTLVKSLAGQLKNITASVRGLGLRKPHQTVDVADTPQ
                     NRGMIYAANHLLKVSE"
     gene            1099..1569
                     /gene="rplO"
                     /locus_tag="VEW08_01370"
     CDS             1099..1569
                     /gene="rplO"
                     /locus_tag="VEW08_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HYJ39404.1"
                     /translation="MRLNDMKPAHGARKTRLRVGRGASAGQGKTCGRGVKGQRARKGG
                     YHKVGFEGGQMPIQRRMPKVGFRSKMEMAEVRLDELLKVEGSEITLDTLKKANIIPTF
                     ADRAKVVLSGEIKKAFSLKGIGATKGAKEAIEKAGGSVEALPEKPKPEKLKAKK"
     gene            1591..2943
                     /gene="secY"
                     /locus_tag="VEW08_01375"
     CDS             1591..2943
                     /gene="secY"
                     /locus_tag="VEW08_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003304058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HYJ39405.1"
                     /translation="MASSSQNPASVLGDAARFGDVRKRLLFLIGGLIVYRIGTYIPVP
                     GIDPERVAGFFRDNAETIFGIVNMFSGGALERLSIFTMGVMPYISASIIVQMMAMVVP
                     QWMEYRKEGESGRRKLTEITRYGTLGLALFQSFITANGLQQQGMVVAPGWQFLFVATI
                     TMTTGAMFLMWLGEQITERGVGNGISMIILGGIVAGIPGAIGQTVDQINTGEMSGVIA
                     IALILMVLGVTTFCVFVERAQRRIPVDYAKRQVGRRMYAGQTTHLPFKLNMSGVIPPI
                     FASSLLLFPATIAGFAGQNTESWWGNALATFSATFGYGQPAHITAYAFLIIFFCFFYT
                     ALVFNARETADNLKKSGAFIRGIRPGQQTGEYIDKVLTRLTLWGAVYITAVCLLPEIL
                     VSRGGVPFVFGGTSLLIVVVVAMDFFSQLQAHLMSHHYPGLLKKANLMGYGRSGAPQS
                     "
     gene            2963..3076
                     /gene="rpmJ"
                     /locus_tag="VEW08_01380"
     CDS             2963..3076
                     /gene="rpmJ"
                     /locus_tag="VEW08_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HYJ39406.1"
                     /translation="MKVRPSVKKICKNCKIVRRRGVVYVICADPRHKQRQG"
     gene            3110..>3381
                     /gene="rpsM"
                     /locus_tag="VEW08_01385"
     CDS             3110..>3381
                     /gene="rpsM"
                     /locus_tag="VEW08_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HYJ39407.1"
                     /translation="MARIAGVNIPMNKHVVIGLTHIFGVGRSRAKDICASTGIAPTTK
                     VKDLTEVEVNAIRSALAKTAVEGDLRRENSMNIKRLMDLGAYRGIR"
BASE COUNT          681 a         1057 c         1056 g          587 t
ORIGIN      
        1 gaaagaacgg cgccagcgcc gagccatcaa gacccgcatg aagatccgcg agctgggcat
       61 tgcccgcctc acggttcatc ggaccccgca gcacatctac gcgcaggtgt tcgattcaca
      121 gggcgccaag gtcatcgcca ccgcgtcgac cgtgcaggaa aaggtcgcca agggcctcaa
      181 gggcaccggc aacgtggagg cggcgaaggc cgtcggcgct gctattgctg aagccgccaa
      241 ggccgcaggc gtcaccaaag tggcgttcga ccgcgcgggc ttcatgtatc acggccgcgt
      301 caaagcgctg gccgacgccg cgcgcgaagc cggcttagag ttttaacagg acctaacaaa
      361 tggcacgacc cgacaacaaa tcccaggctc aggacgaatt cctcgagaag ctggttgcag
      421 tcaatcgcac ggccaaggtc gtcaagggcg gccgccagtt cggtttcacc gcactgacgg
      481 tggtcggtga cggcgccgga cgcgtgggct tcggtttcgg caaggcgcgt gaagtgccgg
      541 tcgcgatctc caaggccatg gcgcaggcgc gcaagaacat ggtcaacgtg gcgctcaaga
      601 acgacacgct gcatttcgcc atcaagggcg cgcacggcgc gacgatggtg tacatgcagc
      661 cggcgtccga cggtactggc gtcatcgccg gcggcggcat gcgcgcggtg ctcgaatgcg
      721 ccggcgtgcg caacgtgctc gccaagagct acggctcgcg caaccccatc aacgtggttc
      781 gcgccacggt gaatgcgctg gccaacgtga attcgcccga gaccatcgcg gcgaagcgcg
      841 gcaagaccct cgacgagatc ctgggctaat ccccagccct ctttcgaacg agatatacga
      901 acatggccaa caaacagatc aaggtcacgc tggttaagag cctcgcgggc cagttgaaga
      961 acatcacggc ttcggtgcgc gggcttggcc tgcgcaagcc gcaccagacg gtggacgtgg
     1021 cggacacgcc gcagaatcgc ggaatgatct acgcggccaa tcatctgctg aaggtttcgg
     1081 agtaatcgca acatgaccat gcgtctcaat gacatgaaac ccgcacacgg cgcccgcaag
     1141 acgcgcctgc gtgtcggacg cggcgcttcc gccggccagg gcaagacctg cggccgcggc
     1201 gtgaagggcc agcgcgcgcg caagggcggt taccacaagg tcggctttga aggcggccag
     1261 atgcccattc agcgccgtat gccgaaagtg ggcttccgct cgaaaatgga aatggccgaa
     1321 gtgcggctcg acgagctcct caaggtcgaa ggcagcgaga tcactctcga caccttgaag
     1381 aaggccaaca tcatcccgac atttgcggat cgcgcgaagg tcgtgctgtc gggcgagatc
     1441 aagaaagcct tctcgctcaa gggcatcggc gccaccaagg gcgcgaagga agcgatcgag
     1501 aaggccggcg gctcagtcga ggctctgccc gagaagccga agcccgagaa attgaaggcc
     1561 aagaagtaag gtccagggaa caggtaagac gtggcatcca gctcacagaa cccggcatca
     1621 gtactcggcg acgcggcgcg tttcggcgac gtacgcaagc gcctgctgtt tctgatcggc
     1681 ggcctgatcg tctatcgcat cggcacgtac atccccgtgc cgggcatcga ccctgagcgc
     1741 gttgcgggct tcttccggga caatgccgag accatcttcg gcatcgtcaa catgttctcg
     1801 ggcggtgcgc tcgagcgcct ctccatcttc accatgggtg tcatgcccta catctccgcg
     1861 tcgatcatcg tgcagatgat ggcgatggtg gtgccgcagt ggatggagta ccgcaaggaa
     1921 ggcgagtccg gccggcgcaa gctcaccgag atcacgcgct acggcacgct gggcctggct
     1981 ctgttccagt cgttcattac cgcgaacggc ctgcagcaac agggcatggt cgtggctccg
     2041 ggctggcagt tcctgttcgt cgccaccatt acgatgacca cgggcgccat gttcctcatg
     2101 tggctgggtg agcagatcac cgagcgtggc gtgggcaacg gtatttccat gatcatcctg
     2161 ggcggcatcg tggccggcat cccgggcgcg atcggccaga cggtggacca gatcaacacc
     2221 ggcgaaatgt cgggtgtcat cgccatcgcg ctcatcctga tggtgctcgg tgtcaccacc
     2281 ttctgcgtgt tcgtggagcg cgctcagcgc cgcattccgg tggactacgc caagcgccag
     2341 gtcggccgcc gcatgtacgc gggccagacc acgcacctgc cgttcaagct gaacatgtcg
     2401 ggcgtgattc cgccgatctt cgcctcgagc ctgctgctct tcccggccac catcgccggg
     2461 tttgcgggcc agaacaccga gagctggtgg ggcaacgcgc tggcgacgtt ctcggcaacg
     2521 tttggttacg gccagccggc gcacatcacc gcgtacgcct tcctgatcat cttcttctgc
     2581 ttcttctata ccgcgctggt gttcaacgcg cgcgagacgg ccgacaacct caagaagtcg
     2641 ggcgccttca ttcgcggcat ccgccccggc cagcagaccg gcgagtacat cgacaaggtc
     2701 ctcacgcggc tcacgttgtg gggcgcggtg tatatcaccg cggtctgcct gctgcccgag
     2761 atcctcgtat cgcgcggcgg tgtgccgttc gtgttcggtg gcacctcgct gctcatcgtg
     2821 gtcgtggtgg ccatggactt cttctcgcaa cttcaggccc acctgatgtc gcaccactat
     2881 ccgggtttgc tcaagaaggc gaacctgatg ggttacggcc gcagtggcgc gccgcaaagc
     2941 taatttttag gactcaagaa ccatgaaagt acgtccctcc gtaaaaaaga tctgcaagaa
     3001 ctgcaagatc gtgcgacgtc gtggcgtcgt gtacgtcatt tgtgctgacc cgcgccacaa
     3061 acagcgccag ggttaattaa gcgaatagct aagagaaggt atcgaaccaa tggcacgtat
     3121 tgctggcgta aacattccga tgaacaagca cgttgtcatc gggctcacgc acatcttcgg
     3181 agtgggtcgt tcccgcgcga aggacatctg cgcatcgacg ggcatcgctc ccaccaccaa
     3241 ggtgaaagat ctcaccgaag tggaagtgaa cgcgattcgt tcggcgctcg ccaagaccgc
     3301 ggtcgaaggc gacctgcgcc gcgagaactc gatgaacatc aagcgactca tggatctcgg
     3361 cgcgtatcgc ggcatccgtc a
//
LOCUS       DASQJS010000033         4839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_961198, whole genome shotgun sequence.
ACCESSION   DASQJS010000033 DASQJS010000000
VERSION     DASQJS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961198"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1692
                     /locus_tag="VEW08_01390"
     CDS             <1..1692
                     /locus_tag="VEW08_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HYJ39408.1"
                     /translation="LEHLKFLVLDEGDEMLQMGFVDDIEFLFQQAPEDKQVALFTATL
                     PHSIRRIAQTHMRDPREISVQSRGEAAPKIRQRYWMVSGLHKLDALTRVLESERFDAM
                     LVFVRTKIETVDLAQRLEARGFAASALNGDMEQRQREQMVTQLKNGKIDILIATDVAA
                     RGLDVERISHVLNYDIPYDSETYVHRIGRTGRAGRTGDAILFVSPRERNMLRVIERVT
                     KQQLEEMKMPSVADVNELRVVKFKEKITAAVMSGAGKEFQSMLEQVQSDGDIPAIEIA
                     AALASMVQGSTPLFLAAKPESEQQKAPPRIDGSVPQQTYRIEVGHAHGVLPGNIVGAI
                     ANEAGIEGKNIGHIDIREDHSYVDLPGLPDEMMASLQDVRVRGEAIRIQRVDSKPDKP
                     KFSGQSRRPDRMQEEDGGRRPYAGGGGSAGKRFDKSSGEKKPYTGGGNRFDKAGEPRP
                     FKSNRSESRNYSGPPRSYAGTPRFKRDDSAPPPAESGDRPSFEGSRPARFSTFAEKPR
                     FASGDKPRFAGKPSFGDKKPEGFKKKYVGKAAGAKGSYKGGKAHRAKSAYSGTKD"
     gene            1782..2378
                     /locus_tag="VEW08_01395"
     CDS             1782..2378
                     /locus_tag="VEW08_01395"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HYJ39409.1"
                     /translation="MKQLGSYLLKAIVRGALIVVPVYLAILLLLKAAKSIVGLVQPIA
                     KLLPDWFPAADVLSILLVLIVCFLIGALVHTAMGRAAKRAIEKSVLERVPGYSLFRAL
                     GQRAAGQGVDSAWQPALIEIEDALVPGFIVETIDDNLLTVFVPSVPTPFAGAIYIIER
                     RRVHPVDVPFTQAIKTISRWGSGSKELVAAMKRAPPAT"
     gene            complement(2430..2984)
                     /locus_tag="VEW08_01400"
     CDS             complement(2430..2984)
                     /locus_tag="VEW08_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39410.1"
                     /translation="MKRSICLVIIGVMTTLSCAAKEPFLKEEVQEVSATVEAIDSENR
                     LLSLRGPAGTATILAGPEIVNFPQIRVGDEVKVKYTVALAAKITKSKATPTTTLDTGV
                     KTAPEGAKPSASVGATVSTTVQIESVDTSFETVTFKRPDGFVRTIAPASPEGKKFIKT
                     LKKGDMVDVSYTEALAISVEPAKK"
     gene            complement(3012..3665)
                     /locus_tag="VEW08_01405"
     CDS             complement(3012..3665)
                     /locus_tag="VEW08_01405"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HYJ39411.1"
                     /translation="MRNVIRAATILSGVLALLSACVVNAPPVAENTPDGLVRVQSKQV
                     DTVYAKPGVSLARYKRVMLDSVELAFKLDWEKRHPEVSESDVTRIRSQGSAVFYEIFS
                     SALTLNNGYPLATQPGADVLRVTASITELDVAATPGSTGTQRMYVVSPSDLTLFMELR
                     DSTTGALLVRAIDREKGRAVGNLQVEGAVSNSAEARKALEMWAGLLRGALDNARAMN"
     gene            complement(3662..4738)
                     /gene="ydiK"
                     /locus_tag="VEW08_01410"
     CDS             complement(3662..4738)
                     /gene="ydiK"
                     /locus_tag="VEW08_01410"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HYJ39412.1"
                     /translation="MNSQTSPDLTRTTLGVLIIGVLLVASLWTMQAFIGPLVWATAIV
                     VATWPLLMWVQRHLGNSRALATTVMVIVMLVIFVVPFWAALGVMLDASVDGVEVVRSY
                     MKTGLGPPPDWLAKVPVAGERLTAKWQTLSAAGPEQFAETIRPYVKSVAAWLLAVTGG
                     FGGLLGHFLLTVIIAGILYAQGEAAAMGVRAFASRLAGQRGDAAVILAGQSVQSVALG
                     VVVTALVQTVLVGLGFFVAGIPHPGLLTAIVFVLCIAQVGPILVLIPAVIWMYSHASG
                     VAATVFLIYSIPMALLDNFLRPILISRGVDLPLILIIAGVIGGLLAFGVIGLFIGPVI
                     LAVTYSSLGVWIADKPASEAKETS"
BASE COUNT          935 a         1582 c         1498 g          824 t
ORIGIN      
        1 ctcgaacatc tcaagttcct cgttctcgat gaaggcgacg agatgctgca gatgggcttc
       61 gtcgacgaca tcgaattcct gttccagcag gctccggaag acaagcaggt ggcgctgttc
      121 accgcgacgc tgccgcattc catccgccgc atcgcgcaga cgcacatgcg tgatccgcgc
      181 gagatcagcg tgcagtcgcg tggcgaagcc gccccgaaga tccgccagcg ttactggatg
      241 gtcagcggcc tgcacaagct cgacgccctc acgcgcgtgc tcgaatccga acgcttcgac
      301 gccatgctcg tgttcgtgcg caccaagatc gaaaccgtcg atctcgccca gcgcctcgaa
      361 gcgcgcggct tcgcggcctc cgcgttgaac ggggacatgg agcagcgcca gcgtgagcag
      421 atggtcacgc agctcaagaa cggcaagatc gacatcctca tcgccaccga cgtggcggcg
      481 cgcggtctcg acgtcgagcg catctcgcac gtgctcaact acgacatccc gtacgactcc
      541 gaaacctacg tgcaccgcat cggccgcacc ggccgcgccg ggcgcacagg cgatgccatc
      601 ctgttcgtaa gtccgcgcga gcggaacatg ctgcgcgtca tcgaacgcgt taccaagcag
      661 cagctcgaag agatgaagat gccgagcgtc gctgacgtca acgaactgcg cgtggtgaag
      721 ttcaaggaaa agatcaccgc ggccgtcatg agcggcgccg gcaaggaatt ccagagcatg
      781 ctcgagcagg tgcaatccga cggcgacatt cccgccatcg aaattgccgc tgcgctcgcg
      841 agcatggtgc agggttcgac gccgctgttc ctcgcggcga agcccgagtc cgaacagcag
      901 aaggcgccgc cgcgcatcga cggcagtgtt ccgcagcaga cctatcgcat cgaagtgggc
      961 cacgcgcacg gcgtactgcc gggcaacatc gtcggcgcca tcgccaacga agccggcatc
     1021 gagggcaaga acatcggcca catcgacatc cgcgaagatc acagctacgt ggatctgccc
     1081 ggcctgcccg atgaaatgat ggcgagcctg caggatgtac gcgttcgcgg tgaagccatc
     1141 cgcatccagc gcgtggattc gaagcccgac aagcccaagt tcagtggcca gtcgcgccgt
     1201 ccggatcgca tgcaggaaga agatggcggg cggcgtccat acgccggtgg cggcggtagc
     1261 gccggcaagc gattcgacaa atcgagcggc gagaagaagc cgtacacggg tggcggcaac
     1321 cgcttcgaca aggccggcga gccacgcccc ttcaagagca atcgctccga gtcgcgcaac
     1381 tactcgggtc cgccacgcag ctacgcgggc acgccccgtt tcaagcgtga tgacagcgcg
     1441 cccccgccgg ccgaaagcgg cgaccggcct tcgttcgaag gcagcaggcc ggcccgcttt
     1501 tcgacgtttg ccgaaaagcc ccgcttcgcc tcgggcgaca agccgcgttt tgccggcaag
     1561 ccctcgttcg gcgacaagaa gcccgaaggc ttcaagaaga agtacgttgg caaggccgcc
     1621 ggcgccaagg gctcgtacaa gggcggaaaa gcccatcgcg ccaagagcgc gtacagcgga
     1681 acgaaagact gatcgtcagc ttctgacgat ttgcggccgg gccccgatat ggggccttgg
     1741 tcgtacgttt ccgccgcgcg attcggtgcg accatgcgcg catgaaacag ctcggctcgt
     1801 acctgctgaa ggcgatcgtg cgcggcgcgc tgatcgtcgt tcccgtctat ctcgcgattc
     1861 tgctgctgct caaggcggcg aaatcgattg ttggcctggt ccagccgatc gccaaactac
     1921 tgccggactg gtttccagcg gcagatgtgc tgtcgatcct gctggtgctc atcgtctgct
     1981 tcctcatcgg cgcactcgtg cacaccgcga tgggccgagc cgcgaagaga gccatcgaaa
     2041 agtcggtgct ggagcgcgtg cccggctact cgttgtttcg tgccctcggg caaagggccg
     2101 ccggccaggg cgtcgacagc gcctggcaac ccgcactgat tgaaattgaa gacgcgctgg
     2161 ttccaggttt catcgtcgaa accatcgatg acaacctgct caccgtattc gtgccgtcgg
     2221 taccgacccc gtttgccggt gccatctaca tcatcgagcg ccgccgtgtg catccagtcg
     2281 acgtcccatt cacgcaggcc atcaagacca tctcccgctg gggaagcggc tcgaaggagc
     2341 tggtggcggc gatgaagcgg gcgccgccgg cgacctgatc aaacgatcgc ccccgcaata
     2401 cttgcgaggg cgaccgcttt gactgttcgc tacttcttgg cgggctccac cgagatcgcc
     2461 agcgcctcgg tgtacgacac gtccaccatg tcgcctttct tcagcgtctt gatgaatttc
     2521 tttccttcgg gtgacgccgg cgcgatggtg cgcacgaagc catccggccg cttgaaggtc
     2581 acggtttcga aggacgtatc caccgactcg atctgcaccg tcgtcgagac tgtcgcaccg
     2641 accgaagcgg aaggcttcgc accctcgggc gcggttttca ccccggtgtc gagcgtcgtg
     2701 gtcggcgtcg ccttgctctt ggtgattttc gccgcgaggg cgacggtgta cttcactttc
     2761 acttcatcgc ccacgcgaat ctgtgggaag ttcacgattt ccgggcccgc caggatggtg
     2821 gcggtgcccg cggggccgcg cagcgacagc aggcgatttt ccgagtcgat ggcttcaacc
     2881 gtcgcagaaa cctcctgtac ttcctctttc aggaaaggtt ccttggcggc acaactcagc
     2941 gtggtcatga cgccgatgat taccagacag atcgaacgtt tcattttcag cttccttctg
     3001 cagtagtttg gtcaattcat tgcccgggcg ttgtcgagcg caccgcgcag caggccagcc
     3061 cacatctcga gcgctttacg cgcctcggcg gaattcgaca cggcgccctc aacctggaga
     3121 ttgcccaccg cgcgaccctt ctcgcgatcg atggcgcgca ccagcagcgc gccggtcgtt
     3181 gaatcacgca gttccatgaa cagggtcagg tcgctgggcg agaccacgta catgcgctgg
     3241 gtgccggtgc tgcccggtgt agcggcaaca tcgagctcgg tgatcgatgc ggtcacacgc
     3301 aggacgtcgg ccccgggctg cgtcgcgagc ggatagccat tgttcagcgt gagtgcggac
     3361 gaaaagattt cgtagaaaac cgcggagccc tgcgagcgga ttctcgtcac gtcgctctcg
     3421 ctcacttccg gatgccgctt ctcccaatcg agcttgaagg cgagctccac ggaatcgagc
     3481 atcacgcgct tgtatctcgc cagtgacacg ccaggcttcg cgtaaaccgt gtccacctgc
     3541 ttcgactgca cacggacgag gccatcgggc gtgttctcgg caaccggcgg tgcgttgacc
     3601 acgcatgcgc tgagcagagc gagcacgcct gagaggatcg tcgccgcccg aatgacattt
     3661 ctcatgaagt ttccttggct tccgaagccg gcttgtccgc tatccacacg ccgagcgacg
     3721 agtaagtcac ggcgaggatc accggaccga tgaacaggcc aatgacgccg aacgccagca
     3781 ggccgccgat gacaccggcg atgatgagga tcagcggcag gtcgactccg cggctgatga
     3841 gtatcgggcg caggaagttg tcgagcagcg ccatcggtat gctgtagatc aggaacacgg
     3901 tggctgccac gccgctggca tgcgaataca tccagatgac cgccggtatc aaaacgagta
     3961 tcggcccgac ctgcgcgatg cagagcacga aaacgatggc cgtgagcagt ccggggtgcg
     4021 gtatgccggc cacgaagaag ccgagtccga cgagcacggt ttgcacgagc gcagtcacga
     4081 cgacgccgag cgccacgctc tgcaccgact gcccggcaag aatcaccgcc gcatcgccgc
     4141 gctgccccgc gagccggctc gcgaacgccc tcacgcccat ggcggccgcc tcgccctgcg
     4201 cataaagaat gccggcgatg atcaccgtga gcagaaagtg cccgagcagg ccaccgaagc
     4261 caccggtcac cgccaggagc cacgcagcca cggacttcac gtaaggacga atggtttcgg
     4321 caaactgctc cggcccggca gcggacaggg tctgccactt tgcggtgagg cgttcgccgg
     4381 cgaccggcac cttcgcgagc cagtcgggcg gcgggccgag gccggtcttc atgtacgagc
     4441 gtacgacctc gacgccgtcg accgacgcgt cgagcatcac gccgagcgct gcccagaacg
     4501 gcacgacgaa gatcaccagc atgacgatga ccatgacggt cgtcgccagg gcgcgactgt
     4561 tgccgaggtg acgctgcacc cacatcagca gcggccaggt ggctacgacg atggccgtgg
     4621 cccacaccag cggcccgatg aatgcctgca tggtccacag gctcgccacc agcagcacgc
     4681 cgatgatgag cacgcccagc gtcgttcgcg tcaggtccgg cgaggtttgt gaattcattg
     4741 cgcatcccgg gatttatttg ttgatttgat accggatgcc gcactcccga tcatgggacc
     4801 aaggtctcat tcccgtaggc ggcccgcagt tcgatattg
//
LOCUS       DASQJS010000034         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_961773, whole genome shotgun sequence.
ACCESSION   DASQJS010000034 DASQJS010000000
VERSION     DASQJS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961773"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..568
                     /locus_tag="VEW08_01415"
     CDS             170..568
                     /locus_tag="VEW08_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39413.1"
                     /translation="MNTHHARQKWFQCFSPDGLITRQEAVNLIHKHAAKARVFEDLDG
                     DPALMVGQNGGRHLVLFTDLSDAQIRNELPTAWLREHQQADMFRDAAEKVANDEQCSS
                     EPTGLPRRGALSRAWDNLKKSLKGVFGQPR"
     gene            complement(556..1146)
                     /locus_tag="VEW08_01420"
     CDS             complement(556..1146)
                     /locus_tag="VEW08_01420"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HYJ39414.1"
                     /translation="MEPLFLGGHPAIDFLNTALTPDGVPVETIPDGRAYLEWLKGAGL
                     LDETEAAKLARRFGAKGLDTAAAEARKMREWARDWLERWRARPSADYGEEIATLNKLL
                     AREQSSHELIARKEGFTLAERPQLESPQSLISLVAMQLAAVLTTEKPSLIRNCAGGGC
                     TLTFIDRTKAHRRLFCSATACGNRAKVAAFRSRQRG"
     gene            1246..2193
                     /locus_tag="VEW08_01425"
     CDS             1246..2193
                     /locus_tag="VEW08_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ39415.1"
                     /translation="MKQLLALFALLLFAPFHSAVAADSTVHHRKITIDGVEVFYREAG
                     PANGPTVVLLHGFPSSSHMFRNLIPALADRYRVIAPDFPGFGESAMPDRAQFQYTFAH
                     FAEITDALLTRLEARRYTLYVMDYGAPVGYRLALLHPERVTGLVVQNGNAYAEGLQEF
                     WTPIKAYWASGSREHREALRAGTTAEATQWQYVDGVRDPTRIDPAAWVYDQARLDRPG
                     NIEIQLDLFYDYRTNVELYPRFQQFFRERKPPTLIVWGANDKIFPPQGAHPYRRDLPA
                     AEFHLLDTGHFALEDKGSEIATLMRDFLDRQVASKVASR"
     gene            2203..2604
                     /locus_tag="VEW08_01430"
     CDS             2203..2604
                     /locus_tag="VEW08_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39416.1"
                     /translation="MNRLRIAGAVVVLVIAAGLTYFYVAAPFLWSESRLREKVLSLTP
                     IGSTHEQVLNAIELKGWHPRRMKAEVENHGYLQQFPGAPPVEVGFTSISGYMGAHYEN
                     PIEPTHVAVHWGFGKDGRLTDVWIWKVTDSI"
     gene            complement(2773..>3008)
                     /locus_tag="VEW08_01435"
     CDS             complement(2773..>3008)
                     /locus_tag="VEW08_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39417.1"
                     /translation="GLVAREIGAVILLAAGVALPVAALAIARYLAPFTERTPLAYWAL
                     ALAGAAAVGVVGVAAARQARTAMSMKPAVALRT"
BASE COUNT          550 a          984 c          899 g          575 t
ORIGIN      
        1 gctcctgtga gtaggtattc agagagtagt cgaacaggat tgaacatttt cgacagccct
       61 tccgggaatc cctatggcgc ggcgactgtg cgacacgtcg cgtctcgcac tcctttcccg
      121 cccttccctg attgaagccg gcgcgcgcgc aactaccatt cgccgccaca tgaataccca
      181 tcacgccagg caaaaatggt ttcagtgttt cagccctgac gggctcataa cgcgccagga
      241 agccgtcaat ctgatccaca agcatgccgc caaggcgcgc gtcttcgaag acctggacgg
      301 tgatccggcc ttgatggtcg gtcaaaatgg cggccgccac ctggtgttgt tcacggacct
      361 ttccgacgca cagataagga acgaactacc gaccgcatgg ctgcgcgaac accagcaggc
      421 cgacatgttt cgtgacgcag ccgaaaaggt ggcgaacgat gaacagtgct cgtcggagcc
      481 cactggtttg ccccgccgcg gcgccttgtc ccgcgcgtgg gacaacctga agaagtcact
      541 caagggtgta ttcggtcaac cccgctgacg actccggaac gcggccacct tggcgcggtt
      601 gccgcaggca gtcgcgctgc agaacaaacg ccgatgtgct ttcgtccggt cgatgaacgt
      661 cagtgtgcaa cctccgcccg cgcaattcct gatcagcgac ggcttctcgg tggttagcac
      721 ggccgccaac tgcatcgcga ccagcgaaat cagcgattgc ggcgattcga gttgcggccg
      781 ctccgcgagc gtgaaccctt ctttgcgggc gatgagctca tggctgctct gttcgcgtgc
      841 cagcagcttg ttcagcgtgg cgatctcctc gccgtaatcg gcgcttgggc gagcgcgcca
      901 gcgctcgagc cagtcccgcg cccactcgcg catcttgcgc gcctccgccg ctgcagtatc
      961 gagacctttc gcgccgaacc ggcgcgcgag cttcgcggct tcggtctcgt ccagcagccc
     1021 cgcgcccttc agccactcga ggtacgcgcg tccatcggga atggtttcga ccggcacgcc
     1081 atcgggcgtc agggccgtgt tcaggaaatc gatggcggga tggccgccga gaaatagtgg
     1141 ttccatggct ggagcgtaac catttatctg aggcttgaca agttaccact cttgcgtaac
     1201 ctttattgtg accattgact ggttacactt ccttcgaggt tcgtcatgaa acagttgctc
     1261 gctctcttcg ctctcctgct tttcgccccc ttccattccg cggttgccgc ggattcgacc
     1321 gtgcaccacc gcaagatcac gatcgacggt gtcgaggtgt tctaccgcga ggccggcccg
     1381 gccaacggac ccacggtcgt gctgctccac ggttttccga gctcctccca tatgttccgc
     1441 aacctgatcc cggcgctcgc cgatcgctac cgcgtgatcg cgcccgactt cccggggttc
     1501 ggcgagagtg cgatgcccga tcgcgcgcaa ttccaataca ccttcgcgca cttcgccgag
     1561 atcacggatg cgctgctgac gcggctcgaa gccaggcgct acacgctcta cgtgatggac
     1621 tacggcgcac cggtcggtta ccggctcgcg ttgctgcatc ccgaacgcgt caccggtctc
     1681 gtggtgcaga acggcaacgc ctacgccgaa gggctgcagg aattctggac gccgatcaag
     1741 gcgtattggg ccagcggttc gcgcgagcat cgcgaagcgt tgcgtgccgg caccacggcg
     1801 gaagccacgc agtggcaata cgtggatggc gtgcgcgacc cgacccgcat cgatccagcc
     1861 gcgtgggttt atgaccaggc gcggctcgac cggcccggca acatcgagat ccagctcgat
     1921 ctcttctatg actaccgcac gaatgtcgag ctgtacccgc gtttccagca attcttccgc
     1981 gagcgcaagc cgccaacact gattgtctgg ggtgcgaacg acaagatctt cccgccgcag
     2041 ggtgcgcacc cgtatcggcg cgatctgccg gctgcagagt tccacctgct ggatacgggc
     2101 cactttgcgc tggaggacaa gggctccgag atcgcgacgt tgatgcgtga cttcctcgac
     2161 cgccaggtcg cctcgaaagt cgcgtcgcgc taatctggct tcatgaatcg attgaggata
     2221 gcgggtgcgg tagttgtact ggtcatcgct gcgggcctga cctacttcta cgtggctgcg
     2281 ccgtttctat ggtctgaaag ccgccttcgc gagaaggtgc tttcactgac gcccatagga
     2341 tcgacgcacg aacaagtgct gaacgcgatt gaattaaagg gctggcaccc aaggcgaatg
     2401 aaggcagagg tcgagaatca cggatacctt cagcagtttc ctggcgctcc acctgtcgag
     2461 gtcggtttca ccagtatcag cggctacatg ggcgctcact acgaaaaccc tatcgagcca
     2521 acgcatgtgg cggtgcactg gggatttggg aaagatggcc gtctgacgga tgtgtggatc
     2581 tggaaggtca ccgacagcat ctaaagctgc ttgacctcct tcaaatcggg gtcggcatcc
     2641 ttccagtacc tgcgcgcctc cgccgtttcg cgctgcgcga attcggtctc gccgcgatgg
     2701 cgagccacca gcgcaagcgc gaggtggcct ccggcatatg cgccatcgtg ctcgaggaat
     2761 aggctacacc cctcatgtac gtagtgcgac cgccggcttc atgctcatcg ccgtgcgagc
     2821 ctgacgcgcg gcggcaactc cgacgacacc gacggccgcg gcccccgcca gtgccagcgc
     2881 ccaatacgcg agcggcgtgc gctccgtgaa cggcgcgaga taacgcgcaa tggccagcgc
     2941 cgcgacgggc aaggccacgc cggcggccag caggatgacg gcgccgatct ccctcgccac
     3001 cagcccgc
//
LOCUS       DASQJS010000035         4121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_651782, whole genome shotgun sequence.
ACCESSION   DASQJS010000035 DASQJS010000000
VERSION     DASQJS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651782"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1609)
                     /locus_tag="VEW08_01440"
     CDS             complement(53..1609)
                     /locus_tag="VEW08_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HYJ39418.1"
                     /translation="MTSPRPRAAKPVLLAAAEVPADFLTAPAPVVETVDAAAETEDSS
                     LTIAGLTPEQYPDVFDRIRAGFKLTDIDSRQIDVQLAFFANNPQYLERVFGRAVPYLH
                     HIVQEIEKRGMPLELALLPVVESAFEPYARSWATANGLWQFMPGTGERFGLKQDWWYD
                     GRRDVMASTRAALDYLQYMHDEFFDDWLLAIAAYNCGEFRVQREVNANRAAGKGVDFF
                     SLRLPSETKAYVPKLLAMRRLVANPEDYGIAFSPIPNEPYFTRVDTGGQLDLTLAAEL
                     AGITLDEVYELNPGFHRWATDPAGPHFLLLPRDTADAFRKNLELLSPDERVRVSLHTV
                     KSGESVASIAKKYKTQAVVVRDMNTLDANAPLVVGSELRVPTAVVNLPAKVMLAAARV
                     DGSGGRTGRRPGVHVVRSGDSLWRIAKRNRMDVATLARLNGMGPGDTLRAGQKLVLNT
                     RAPASGNRSTTPSANSRAVSYKVRSGDTLSRIAKVFGVTVSDLVNWNGISKHSMLRPG
                     QKLTVNVRRH"
     gene            2004..2657
                     /locus_tag="VEW08_01445"
     CDS             2004..2657
                     /locus_tag="VEW08_01445"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ39419.1"
                     /translation="MSEALDDVFGWELLQLGTWGPREGLLSGCRTRRHAVIAHGRFKS
                     ADADIGARLSQLPIANGAVDAVVLPHTLEFETDPYAVVREADRVLAGEGQLVVLGFRP
                     FSLWGFRSRAISRGYPPGLRRMLGAGRIADWLELLGYDVTLTRKYLFTPPWGGSAPRP
                     GVPSALLRRGWIKPWPSGAYLIKARKRVYTLTPIRPKTRERPQTIGGLVKPTTRSSS"
     gene            2688..3434
                     /gene="dnaQ"
                     /locus_tag="VEW08_01450"
     CDS             2688..3434
                     /gene="dnaQ"
                     /locus_tag="VEW08_01450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HYJ39420.1"
                     /translation="MRQIVLDVETTGLEAAAGHRIIEIGCVEMLNRRTTGQKFHRYLN
                     PEREIDAGAMAVHGIDSARLQQAPKFAEVADELLAFINGAELIIHNAPFDVGFLNAEF
                     ARLPGDVRTVATVCKVLDTLALARSLHPGQRNSLDALCKRYSIDNTKRELHGALLDAG
                     ILVDVYLAMTGGQSALALDMNGRGSEARNHGVAAPARAPGVTLIVTTANSDELQRHEA
                     MLDLIHKSSGGKAVWRAASPPDSDQTHRNH"
     gene            3528..>4121
                     /locus_tag="VEW08_01455"
     CDS             3528..>4121
                     /locus_tag="VEW08_01455"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HYJ39421.1"
                     /translation="MELSMRGCRIGVVGLGYVGLPLAVEFGKHFDTVGFDIKANRIAE
                     LKKGRDSTLECSAAELKSARKLRYSTNLKDLGKCRVFIITVPTPIDRYNRPDLTPLER
                     SSETVGRVLKKGDVVVYESTVYPGCTEEVCIPILERESGLKFNKDFFAGYSPERINPG
                     DKEHRLPSIKKVTSGSTPEAATFVDDLYRTIVRAGTHK"
BASE COUNT          770 a         1264 c         1355 g          732 t
ORIGIN      
        1 ttgagttagc gaggccgcat tctgccagcc tcgccctccg ccggggacga aatcagtggc
       61 ggcgaacgtt gaccgtgagt ttctggcccg gacgcagcat cgagtgcttg ctgatgccgt
      121 tccagttgac caggtcggag acagtcacgc cgaagacctt ggcgatgcgc gacaacgtgt
      181 cgccgctgcg caccttgtac gaaacggcgc gcgagttggc ggacggtgtc gtggagcgat
      241 tgccggacgc cggcgcgcgg gtgttcaaca ccagcttctg gccggcgcgc agggtgtcgc
      301 cgggacccat gccgttgaga cgcgcgagcg tcgcgacgtc catgcggttg cgcttggcga
      361 tgcgccacag cgaatcgccg ctgcgcacca cgtgcacccc ggggcgacgc ccggtgcggc
      421 caccggagcc gtcgacgcgc gccgcggcga gcatcacctt ggcgggtagg ttgacgacgg
      481 cggtgggcac gcgcagctcg ctaccaacca ccagcggtgc gttggcgtcg agggtgttca
      541 tgtcgcgcac cacgacggcc tgcgtcttgt atttcttcgc gatggaagcc accgactcgc
      601 cgctcttgac ggtatgcagt gaaacgcgca cgcgttcatc ggggctcagc agttcgaggt
      661 tcttgcggaa cgcatcggcg gtgtcgcgcg gcagcagcag gaaatgcggg ccggcaggat
      721 ccgtcgccca gcggtggaag ccgggattga gctcgtacac ttcatcgagc gtgatgccag
      781 cgagttccgc ggccagcgtc aggtcgagct ggccacccgt atccacgcgc gtgaaatagg
      841 gttcgttcgg aatggggctg aacgcgatgc cgtaatcctc ggggttggcc acgaggcggc
      901 gcatggcgag cagcttcggc acgtaggcct tcgtctcgga gggcaggcgc aggctgaaga
      961 aatccacacc tttgccggcg gcgcgattgg cattcacttc tcgctgcacg cggaactcgc
     1021 cgcagttgta ggcggcgatg gccaacaacc agtcgtcgaa gaattcatcg tgcatgtact
     1081 gcaggtagtc gagcgcggcg cgtgtcgaag ccatgacgtc gcggcggccg tcgtaccacc
     1141 agtcctgctt gagtccgaag cgctcgccgg tgccgggcat gaactgccac agaccgttcg
     1201 cggtggccca tgagcgggcg tagggctcga aggcgctctc gaccacgggt aacagggcga
     1261 gctcgagcgg catgccgcgc ttttcaattt cctggacgat gtggtgcagg taaggaaccg
     1321 cacggccgaa tacgcgctcg aggtattgcg ggttgttggc gaagaacgcg agctgcacgt
     1381 cgatctggcg cgagtcgatg tccgtgagct tgaaaccggc gcggatgcga tcgaaaacgt
     1441 cggggtactg ttcgggggtc aggccggcaa ttgtgagcga cgaatcttcc gtctcggcgg
     1501 cggcgtcgac ggtctcgacc accggagccg gggcggtcag gaagtcggca gggacttctg
     1561 cagccgcgag cagtaccggc ttggcggccc tggggcgcgg gctggtcaca gggctctggg
     1621 tagccgccgg gttgggctgg gccacagggg taggtgcagg cacctctgcc gcgctaacgg
     1681 gcgcaggagc gtctttcgtg acttcggggg cagtagcgca gcctgtcagg gccgcggcga
     1741 gaaaactgat gggcgcaata aggcgccacg ccgagaatcc agaactcaaa tcacaccccc
     1801 atggggaacg tcgcaggacc gctttacgaa atatgacggt acttcttcga tgggcagtat
     1861 ttaagctgta agttcacaag tttgctacct tttttgtgat gtaattccca gcgtttgtga
     1921 cgcactccac cgcatctgcc gcagtcgaat cctggctcgc ttccggactg ggtcaggcta
     1981 tgctacaggc cgaatccgag ctcatgtcag aggcattgga cgacgttttc ggctgggagt
     2041 tgcttcaact tggaacttgg ggcccgcgcg aggggctgtt gtcgggttgt cgcacgcgca
     2101 gacacgcggt tatcgcccac ggaaggttca aaagtgccga tgccgacatc ggtgcccggc
     2161 tatcacaatt gccgattgcc aatggcgcgg tcgacgccgt ggtcctgccg cataccctcg
     2221 aattcgaaac cgacccctat gccgtcgtgc gcgaagcgga tcgcgtgctg gccggtgaag
     2281 gtcagctggt cgtgctcgga ttccggccgt tcagcctctg gggatttcgc agccgtgcga
     2341 tcagccgtgg ctacccaccg ggtctcaggc gaatgctggg ggcgggccgc atagccgact
     2401 ggctcgagct gctgggctac gacgtaacgc tcacgcgcaa atacctgttc acaccaccgt
     2461 ggggcggcag tgcgccacgg ccaggggtcc catcggcatt gctacgccgt gggtggatca
     2521 agccctggcc ctcgggtgcg tacctcatca aggcgcgcaa gcgggtctac acgctgacgc
     2581 cgatccggcc gaagactcgg gaacgtccgc agaccattgg cggcctggtg aagccgacga
     2641 cgcgttcgag ttcgtaagtt cgcgatgttc ctctagactc gcgcgccatg agacagatcg
     2701 ttcttgacgt cgaaaccacc ggcctcgaag cggccgccgg ccaccgcatc atcgaaatcg
     2761 gttgcgtgga aatgctgaat cgccgcacga cgggtcagaa gttccatcgc tacctgaatc
     2821 ccgaacgcga aatcgatgcc ggcgcgatgg ccgtgcacgg aatcgattcc gcccggctgc
     2881 agcaggcacc gaagttcgcc gaagtcgccg atgaactgct ggcattcatc aacggcgccg
     2941 aactcatcat tcataacgcc cccttcgatg tcggcttcct caatgccgag ttcgcgcggc
     3001 tgcccggcga tgtccgtacc gtggccaccg tatgcaaggt gctcgacaca ctggcgctgg
     3061 cgcgcagcct gcatcccggg cagcgcaaca gtctcgatgc gctctgcaag cgctacagca
     3121 tcgacaacac caagcgcgag cttcacggcg cattgctcga tgccggaatt ctcgtcgacg
     3181 tgtacctcgc catgaccggc ggccagagcg ccctggcgtt ggacatgaac gggcgcggct
     3241 cggaagcgcg caatcacggc gtggccgcac cggctcgcgc ccctggcgtc accctgatcg
     3301 tgaccaccgc caactccgat gagctgcaac ggcacgaagc catgctcgac ctcatccaca
     3361 aatcgagcgg tgggaaggcg gtttggcgcg cggccagccc gccggatagc gatcaaacgc
     3421 acagaaatca ctgatcagcg ggtgggtggc ctcgctttcg gagccccggc gacggcaaga
     3481 tcgcagccgc atttgtatcc aagcgcttaa aagagaacgg aaaaatcatg gaattgagca
     3541 tgagaggctg ccgcatcggc gtggtcggac tgggctacgt gggtctgccg ctggctgtgg
     3601 agttcggcaa gcatttcgac acggtcggat tcgatatcaa ggcgaatcgc atcgccgaat
     3661 tgaagaaggg ccgagacagc accctggaat gctcggccgc ggaattgaaa tccgctcgca
     3721 aactcaggta ttcaaccaac ctcaaggacc tcggcaagtg tcgggtgttc atcattaccg
     3781 tccccacgcc gatagacagg tacaaccgcc cggacctgac tccgctcgag cgttcgagtg
     3841 aaacggtcgg ccgggtgctc aagaaaggtg acgtggtggt gtacgagtcc accgtctacc
     3901 ctggctgcac cgaagaagtg tgcatcccca tcctcgagcg cgagtcgggt ctcaagttca
     3961 acaaggattt cttcgcgggc tacagcccgg agcgcatcaa cccgggcgac aaggagcatc
     4021 gcctgccgag catcaagaag gtgacgtcgg ggtccactcc ggaagccgca actttcgttg
     4081 acgaccttta tcgcaccatc gtgcgcgccg gcacgcacaa g
//
LOCUS       DASQJS010000036         9393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4421071, whole genome shotgun sequence.
ACCESSION   DASQJS010000036 DASQJS010000000
VERSION     DASQJS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9393
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4421071"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="VEW08_01460"
     CDS             complement(<1..1363)
                     /locus_tag="VEW08_01460"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HYJ39422.1"
                     /translation="MNRFASAAIAALMACLCSASQADEGMWTFHGFPFAKANAALKTS
                     LDQAWLDRVRSATVRLSNCTATFVSAEGLILTNHHCVEACLAEQSSKEKSFVEDGFLA
                     KTREEEKKCQTQVADVLVGMEEITAKISAAIAGKDEAAANDVRKSTLTQLEGECEAQT
                     KQKCQSVTLYEGGQYWLYRYKRYTDVRMVFAPEASIAAFGGDPDNFQFPRWCLDMGLL
                     RAYEDGKPAVPATFLKINFAGPAAGEAVFVSGHPGATDRGLTLAQLKQQRNIDLPQWL
                     LRYSEIRGRFIQFASVSPQNERITADQLNGLENGIKVRRKMLDALHEDTLLERKAAEQ
                     ATLEKNIAKDATLNKNTGNPWKDIDAALAAERGLFLPWNYIENGAGFQGRLVNYARTL
                     VRGAAERDKPNGERFREYTDTALPRVQQNLGAAVPVYTELEEIRLGNGLERMREWLGP
                     DHPM"
     gene            1509..2813
                     /locus_tag="VEW08_01465"
     CDS             1509..2813
                     /locus_tag="VEW08_01465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ39423.1"
                     /translation="MNDQPGPTPPARRFGRIMLAPGYLPSHAVTYLYAAFITIGLLAF
                     VSYMQPYLLTVNLGLAPHLQGRTVSALNFANELLALCLVAPFGALADKIGRRSVYAFG
                     FLWLAAGFLWYPMARTVPQLAGCALFFSVGVAAIGTMLGTVLADTPEEESRGRMVGLT
                     GFCQGLGAAVLVLLLGRLPKYLADSGVDALTAGRITLWLASALCALSSVVVFVGLKRG
                     TPSVLAPAMPLRRILADGLAAARRNPRIWFAYLLQFGSFGDRVVLGTFLSLRLQQAWL
                     ERNVSMADAAARARLPFVVAMAAGLATALIVGALLDRVDRLAVGVAAMALAAVAYLLC
                     GFIADPTQDALMAAVAMLLGAGQIAAIIAGQTLLGQEAPRDVRGAVFGLAGICASAGI
                     LFINAFGGWLYDSVSKGGPFFLLAGVNFAIVLFGVRLYGRNR"
     gene            complement(2810..3529)
                     /locus_tag="VEW08_01470"
     CDS             complement(2810..3529)
                     /locus_tag="VEW08_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39424.1"
                     /translation="MLGKFHEISIETANIAESVAFYERLGFSHCGTTDTWQHPYGVLT
                     DGRLYIGLHQFKFPSPTLTYVQPGVAQRAHVIAKVGIDIAWKRVSDEAFNEFGFLDPS
                     GQAVRVQEAPTHFASDRDRGETSLCGDFAEYSLPYSEFEPMRAFWEPLGFVALGESDA
                     PYVRMSMTSDHIDLAVHRPRTLDQPMLVFAAPDMGERIERLRSLDVAMSDDLPRGLDP
                     RSGALLRAPEGTALLLVNAVD"
     gene            complement(3583..5037)
                     /locus_tag="VEW08_01475"
     CDS             complement(3583..5037)
                     /locus_tag="VEW08_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HYJ39425.1"
                     /translation="MIKLKDSSLWKELCLIDGKWLPADSGRTTEIHNPANGELLGTVP
                     VMVAGESRRAIEAAHAAMPAWSKKTAGERAKIMRRWFDLMLANVDDLAVIMTAEQGKP
                     LAESKGEITYAASFIEWFAEEGKRIYGDIIPGHQADKRIMVLRQPIGVVAAITPWNFP
                     AAMITRKAAPALAAGCTFVCKPALQTPYSALAMGELAQRAGIPPGVFNVVTGDAAAIG
                     GEFTGNDKVRKVSFTGSTPVGKLLMSQCAGTVKKVALELGGNAPFLVLDDADLDAAVT
                     GAIQSKYRNTGQTCVCANRFIVQSKIYDAFAQKLTEAVRGLRVGDGLKGETDQGPLID
                     AKALAKVEEHVADARAKGATIVTGGERHALGGTFYEPTVLTNVSAKMTLAREETFGPV
                     APLFRVETDEEAVRLANDTEFGLAAYVYTRDLTRSWRVTEALEYGIVGLNTGLISTEV
                     APFGGVKESGIGREGSKYGILEFTELKYICVGGI"
     gene            complement(5034..5705)
                     /locus_tag="VEW08_01480"
     CDS             complement(5034..5705)
                     /locus_tag="VEW08_01480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ39426.1"
                     /translation="MGEFTTLMARDGHEFNAWLTAPSQPARGAVVIAQEIFGVNRHIR
                     AVADGFAADGYVTIAPCLFDRVRRGIELGYSEAEVQQGRGYRLQIPKEKTLLDLTASI
                     NVIKHAGRVSIVGYCWGGTLAYIAACELPVFCAVSYYGGQIKDHLEKSPRRPVLYHFG
                     EKDPHIPLSDVDKIRAADPNGIFHLYPADHGFNCDERSTYDAASAALARGRTLQFLTA
                     QMERK"
     gene            5818..6297
                     /locus_tag="VEW08_01485"
     CDS             5818..6297
                     /locus_tag="VEW08_01485"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYJ39427.1"
                     /translation="MQTRLLVALILNVVAFLTLPTPALSNGQASPSMAELTEQVRATE
                     IAFAKTLADRDVKAFTSMIAPDVIWLADVPLRGPAAVLASWQKFFDAPTPPFSWAPEI
                     VEVQEGGKLALSTGPVFSPEGKRVATYTSIWRREKSGKWLIIFDRGSPDCDCASDKK"
     gene            6309..6791
                     /gene="ybaK"
                     /locus_tag="VEW08_01490"
     CDS             6309..6791
                     /gene="ybaK"
                     /locus_tag="VEW08_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HYJ39428.1"
                     /translation="MATDYPVTMAIRALRQHKVDFTPHLYTWEPRGGTRASAEHLGVD
                     EHQVVKTLIFEDDAKRPLCILMHGDREVSAKNLARQLNHKSVAPCAPEVADRHSGYQV
                     GGTSPFGLKRAMPITCERAIAELPLIYINGGARGFLVGISPADLVRVLKPRLVEVAIV
                     "
     gene            complement(6781..7152)
                     /locus_tag="VEW08_01495"
     CDS             complement(6781..7152)
                     /locus_tag="VEW08_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39429.1"
                     /translation="MMSRLAFYFVEALLLALVGCGALFVARGALQRLGWNLKPRWPWV
                     YAWACGLLLALGIAGGELRGPEFLLVYSLVGKLLLFAVLAWLVSFPLSRYVQITPRRE
                     SPHDTGAATPAPRSQDPVVRQ"
     gene            complement(7149..8732)
                     /locus_tag="VEW08_01500"
     CDS             complement(7149..8732)
                     /locus_tag="VEW08_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HYJ39430.1"
                     /translation="MISDLRSFPAGHVLEADVCIIGGGAAGISMAMQFHGTKTRVCLL
                     EGGGEHMEADSQALYHVDNEGQVPVQGMQSRLRMFGGTTNHWTGRCAPLDAVDFAARS
                     WVPHSGWPITRADLDPFYERAQGICDLGNYFYDEDVLEALGVPDMQLDRDKLEPQCWQ
                     MSGPTRFAGKYGAVLRVVENIQVVLHATATSVALNPEGTQVEHIALSTPEGTRHRVKA
                     RYYVLAAGGIENPRLLLMSDDVQKTGVGNQRDLVGRFFMEHLRVDATLVMEGDPHLVS
                     RMYARHDSSFGNYVVGVRLCDRFQEASKVLNGSVTTYREGGLDPDSGTSSLTRLADEL
                     KSGHWPKDAGREIFNVVADFSDVALNVRRRFLRPGAEPYSRTLRQILLEGEQSPNPSS
                     RIRLAEATDALGMRRVMADWRLTELDRHSTQLLVLTVATEMARLYKARMSIPFWLGDS
                     QTDWSRHLRDVAHHIGTTRMADSEQKGVVDADCRVFGVPNLFVAGSSVFPTGGQANPT
                     LTIVALALRLADHLKGKLA"
     gene            complement(8729..9205)
                     /locus_tag="VEW08_01505"
     CDS             complement(8729..9205)
                     /locus_tag="VEW08_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39431.1"
                     /translation="MESRAQDRQSSRERPEIMPIIDTNPGSEDCRDPWSRRSLIRSGL
                     ASLLAPALVAIAVPARPQNATNDLLRGAYHSIEDARAIGRSYLQAMPHEADLETLAQA
                     LFGANLPANTRELRSRLAASRDADFGAGRVLAVRGWLMAVTEARWCALAVFLGDTA"
BASE COUNT         1524 a         3008 c         3089 g         1772 t
ORIGIN      
        1 ccatggggtg gtccgggcct aaccactcgc gcatgcgttc gaggccgttg ccaaggcgta
       61 tttcctcgag ctcggtgtac acgggcacgg cggcgccgag gttctgctga acgcgcggca
      121 gcgcggtgtc ggtgtattcg cggaatcgct cgccgttcgg cttgtcacgc tccgcggcgc
      181 cgcgcaccag cgtgcgcgcg tagttcacga gccggccctg gaatcccgcg ccgttctcga
      241 tgtagttcca gggcaggaac agaccgcgct cggcggccag ggccgcgtcg atgtccttcc
      301 acggattgcc cgtgttcttg ttgagcgtgg cgtccttcgc gatgttcttc tcgagcgtcg
      361 cctgttcggc cgctttgcgc tcgagcagcg tgtcctcgtg cagcgcgtcg agcatcttgc
      421 gacgcacctt gatgccattc tcgagaccgt tgagctgatc ggcagtgatt cgctcgttct
      481 gcgggctcac gcttgcgaac tggatgaagc ggccgcggat ttccgagtac cgcagcagcc
      541 actgcggcag gtcgatattg cgctgctgct tgagctgcgc cagcgtcagc ccgcggtcgg
      601 tggcgccggg gtggccggac acgaacaccg cttcacctgc cgcaggaccg gcgaagttga
      661 tcttgaggaa ggtggccggc acggcgggct tgccgtcttc ataggcgcgc agcaggccca
      721 tgtcgaggca ccagcgcgga aactggaaat tgtcggggtc gccgccgaag gccgcgatgg
      781 acgcctcggg cgcgaacacc atgcgcacgt cggtgtagcg tttgtatcta tataaccagt
      841 actgcccgcc ctcatacagc gtgacgctct ggcatttctg cttcgtctgc gcctcgcatt
      901 caccttcgag ctgcgtcagt gtggatttgc gcacgtcgtt ggcggccgct tcgtctttgc
      961 cggcgatggc tgcggatatc ttcgcggtga tttcttccat tccgacgagc acgtcggcca
     1021 cctgcgtctg gcacttcttc tcttcctcgc gggtcttggc gaggaagccg tcctccacga
     1081 agcttttttc cttcgacgat tgctccgcga gacaggcttc gacgcagtga tgattggtga
     1141 ggatcagtcc ctcggcggac acgaacgtag cggtgcagtt ggacaggcga acggtggccg
     1201 agcgcacgcg atcgagccat gcctggtcga ggctggtctt gagcgccgcg ttggccttcg
     1261 cgaacgggaa accgtggaaa gtccacatgc cttcgtcggc ctgggaggcg gaacacaggc
     1321 aggccatcag tgcggcaatc gccgcgctcg caaatcggtt catggaagga actccgggga
     1381 ttttttggtg ccgcctgacg cggtcgatgc gcggattatc tccacaattg gcagcttgcg
     1441 catgtccggc acgccgcgtc cctacctcgg ttcggcgcgg gcagcgggcg tggcatcatg
     1501 ggaccagcgt gaacgatcag cctggcccga ctcctcccgc gcgccgcttc ggcaggatca
     1561 tgctcgcgcc gggctatttg ccgtcgcacg ccgttaccta tctatatgcg gcgttcatca
     1621 cgatcgggtt gctcgcattc gtgagttaca tgcagccgta cctgctgacc gtgaacctgg
     1681 ggctcgctcc ccatctgcag ggccgcacgg tttccgcgct caatttcgcc aacgagctcc
     1741 tggcgctttg cctggtcgcg ccattcggtg cgctggcgga caagatcgga cggcgctccg
     1801 tgtacgcctt cgggttcctg tggcttgccg ccggttttct ctggtatccc atggcgcgca
     1861 cggtcccgca gctcgctggt tgcgcgctgt tcttctcggt gggcgtcgcc gccatcggca
     1921 cgatgttagg cactgtgctg gcggacactc ccgaagaaga atcacgtggc cggatggtcg
     1981 ggctcacggg tttctgccag gggctgggcg cagccgtgct ggttctgctg ctcggacggt
     2041 taccgaagta cctggccgac tcgggggtcg atgcactcac ggcggggcgg atcacactgt
     2101 ggctcgcctc cgcgctgtgc gcgctgtctt cggtagtggt gttcgtgggc ctgaagcggg
     2161 gcacgcccag cgtgcttgcg ccggccatgc cgctccgtcg catcctggcg gacggccttg
     2221 cggcggcgcg gcgcaatcct cgcatctggt ttgcctacct gctgcagttc gggtccttcg
     2281 gcgaccgtgt ggtgctcggc acgtttctgt cgctgcgcct gcagcaggcc tggctcgaac
     2341 ggaatgtcag catggccgat gccgccgcac gggcgcggct gccattcgtc gtggcgatgg
     2401 ccgcgggcct ggccaccgcg ctcatcgtcg gtgcgctgct cgaccgcgtg gatcgcctgg
     2461 cggtcggtgt ggccgccatg gccctcgcgg cggttgccta tctgttgtgt ggcttcatcg
     2521 ccgatccgac gcaggatgcg ctcatggcgg cggtggcgat gttgttaggc gcggggcaga
     2581 tcgcggccat catcgcgggc cagacgctgc tcggccagga ggcgcctcgc gacgtacggg
     2641 gcgcggtgtt cggcctcgcc ggcatctgcg cgtcggccgg cattctcttc atcaacgcgt
     2701 tcgggggctg gctctacgat tcggtgtcga aaggcggccc gttcttcctg ctcgcgggcg
     2761 tcaacttcgc catcgttctg ttcggcgttc gcctgtacgg acgaaatcgc taatcgacgg
     2821 cgttgacgag aagcagcgcc gtgccttcgg gtgcgcgcaa cagcgcgccc gagcggggat
     2881 cgaggccgcg cggcaggtca tccgacatcg ccacgtcgag tgaacgcagg cgctcgatgc
     2941 gttcacccat gtccggggcg gcgaacacca gcatgggctg gtccagggtg cgcggacggt
     3001 gtaccgcgag atcgatgtgg tcgctggtca tcgacatgcg cacgtagggc gcgtcggatt
     3061 ccccgagcgc gacgaacccc agcggttccc agaacgcacg catgggttcg aactcggaat
     3121 acggcaggct gtactcggcg aagtcgccgc acagcgaagt ctcgccgcga tcacggtcac
     3181 tggcgaaatg cgtgggtgct tcctgcacgc gtactgcctg tcccgacggg tcgagaaaac
     3241 cgaactcgtt gaaggcctcg tcgctgacac gcttccaggc gatgtcgatg cccactttcg
     3301 cgatgacgtg cgcgcgttgc gcgacgcccg gttggacgta cgtgagcgtc ggcgagggaa
     3361 acttgaactg gtgcaggccg atgtagagcc gcccgtcggt caggactccg tacggatgct
     3421 gccaggtgtc ggtggtgccg cagtggctga aaccgagccg ctcgtagaaa gccaccgatt
     3481 cggcaatgtt ggccgtctcg atggagattt cgtgaaactt tcctaacact ggcgcgggag
     3541 tttacgcatc ttgcagcgcg ctgcatatgc acgcgcgcta cgtcagattc cacccacgca
     3601 gatgtacttg agctcggtga attccaggat gccgtacttc gaaccttcgc gaccgatacc
     3661 gctttccttg acaccgccga acggcgcaac ctcggtggag atgagacccg tgttgagtcc
     3721 gacgatcccg tactccagcg cctcggtgac gcgccaggaa cgcgtgaggt cccgcgtgta
     3781 cacataagcg gcaagcccga actcggtgtc gttcgcgagc cgcaccgctt cttcgtccgt
     3841 ctccactcga aacagaggcg ccacggggcc aaaggtctct tcacgggcca gcgtcatctt
     3901 cgcgcttacg ttggtgagca ccgtgggttc gtagaatgtt ccgccgagcg catgacgctc
     3961 tccgccggtg acgatggttg cgcctttggc cctggcatcg gcgacgtgtt cctcgacctt
     4021 ggccagggcc ttggcgtcga tcagcgggcc ctggtccgtt tcgcctttca atccgtcgcc
     4081 cacgcgcaag ccgcgaaccg cctcggtcag cttctgcgca aatgcgtcgt agatcttcga
     4141 ctgtacgatg aagcgattgg cgcacacgca ggtctggccg gtgttgcggt acttgctctg
     4201 gatggcgccc gtcaccgcgg cgtcgaggtc ggcgtcgtcg agcaccagga acggcgcgtt
     4261 gccgccgagt tccagcgcca ctttcttcac cgtgccggcg cactgtgaca tgagtagttt
     4321 gcccacgggt gtcgagccgg tgaacgacac cttgcgcact ttgtcattgc cggtgaactc
     4381 gccgccgatg gcggctgcgt cgccggtgac cacgttgaat acgcccggag gaataccggc
     4441 acgttgcgcc agttccccca tcgccagcgc ggagtacggc gtttgcagcg ccggcttgca
     4501 gacgaacgtg caacccgcgg cgagcgccgg cgcggccttg cgagtgatca tcgcggcggg
     4561 aaaattccac ggcgtgattg cggcgaccac gccaatcggc tggcgcagca ccatgatgcg
     4621 tttgtcagcc tggtgcccgg gaatgatgtc gccatagatg cgcttgcctt cctcggcgaa
     4681 ccattcgatg aacgaggccg cataggtgat ctcacccttc gattccgcca gcggcttgcc
     4741 ttgctcggcg gtcatgatga cggcgagatc gtcgacattc gcgagcatca gatcgaacca
     4801 gcgccgcatg atctttgcgc gctcgccggc tgttttcttc gaccacgcgg gcatggcggc
     4861 gtgcgcggcc tcgatggcgc gtcgcgattc tcccgccacc atcaccggaa ccgtacccag
     4921 caactcgcca ttggcggggt tgtggatttc agtggtacgt ccggaatcgg cgggcagcca
     4981 cttgccgtcg atgaggcaca gctctttcca caacgatgaa tccttgagct tgatcatttg
     5041 cgttccattt gcgcggtcag gaattgcagc gtgcgcccgc gcgcgagcgc ggcgctggcc
     5101 gcatcgtagg tgctgcgttc gtcgcagttg aacccgtggt cggccggata gaggtgaaaa
     5161 atgccgttcg ggtcggcggc gcggatcttg tcgacgtcgc tcagcggaat gtgcggatct
     5221 ttctcaccga agtggtacag caccggccgg cgcggagatt tctccaggtg atccttgatc
     5281 tgtccgccgt aatacgacac ggcgcagaac accggcagct cgcaggccgc gatgtatgcc
     5341 agtgtgccgc cccagcagta acccacgatc gacacgcgac cggcgtgttt gatcacattg
     5401 atgctggcgg tgagatcgag aagggtcttc tccttgggaa tctgcaaccg gtagccccgg
     5461 ccctgctgaa cttccgcttc gctgtagccg agttcgatgc cgcggcggac gcggtcgaac
     5521 aaacaaggcg cgatggtcac gtagccgtcc gcggcgaagc cgtcggccac cgcgcggatg
     5581 tgccggttca cgccgaagat ttcctgcgca atcaccacgg cgccgcgcgc aggttgactg
     5641 ggggcggtga gccaggcgtt gaactcgtgg ccatcgcgcg ccatgagggt ggtgaactca
     5701 cccatggggt gtgctcctta acgggattcg tcgagaggat gacattgtgc caccagtggg
     5761 gcggatgccc gctctctcga aggtattatc gccgtggtcc ttggtaggga gattccgatg
     5821 caaacccgcc ttctcgtggc actgatcctc aatgtcgtgg cctttctgac gttaccaaca
     5881 ccggcgctct cgaatggcca ggcaagccca tccatggccg agctcaccga acaggtacgg
     5941 gccaccgaga tcgccttcgc aaaaaccctg gcggaccgcg acgtaaaggc cttcacgagc
     6001 atgatcgcgc cggacgtcat ctggcttgct gacgtgccgc tgcgaggacc cgccgccgtg
     6061 ttggcgagct ggcagaagtt tttcgatgcg ccaacgccac cattttcgtg ggcgccggag
     6121 atcgtcgaag tccaggaagg cggcaagctg gcgttgagca ccgggccggt gttcagtccc
     6181 gagggaaaac gcgttgccac ctacacgtcg atctggcgcc gtgagaagtc cggcaaatgg
     6241 ctgatcatct tcgatcgcgg cagcccggac tgcgattgcg cgtcggacaa aaaatagctt
     6301 accggggtat ggccaccgac taccccgtca ccatggcgat ccgcgcgctt cgccagcaca
     6361 aggtcgactt cacgccgcac ctgtacacgt gggaaccgcg cggcggtacg cgtgcgtccg
     6421 ccgagcacct cggggtcgat gaacaccagg tggtgaagac cctgatcttc gaggatgacg
     6481 cgaagcgccc gctctgcatc ctcatgcatg gcgatcgtga agtgtcggcg aagaacctgg
     6541 cgcgccagct caatcacaag agtgtcgcgc cctgcgcgcc cgaagtcgcg gaccggcact
     6601 ccggctacca ggtgggcggc acttcgcctt tcggtctcaa gcgcgcaatg ccaatcacct
     6661 gcgaaagggc catcgcggaa ctgccactca tctatatcaa cggtggggcg cgggggttcc
     6721 tggtgggcat cagcccggca gacctggtgc gggtcctgaa accccgcctg gtcgaggtcg
     6781 ctattgtctg accaccgggt cctggctccg cggcgccggc gttgctgcgc cggtgtcatg
     6841 aggtgattcc cggcgcggag tgatctgtac gtagcgcgac agcgggaacg aaaccagcca
     6901 ggcgagcact gcgaacagca gcaatttccc gaccagcgaa tacaccagca ggaattccgg
     6961 gccgcgcagt tcgccgcccg cgatgccgag ggcgagcaac aacccgcatg cccacgcgta
     7021 aacccagggc caacgcggtt tcaggttcca cccgagcctt tgcaatgcac cgcgtgcgac
     7081 gaacagcgcg ccgcagccca ccagcgcgag gagcagtgct tcgacgaagt agaacgccag
     7141 gcgcgacatc atgcgagctt gcccttgaga tggtcggcaa gccgcaacgc cagcgcgacg
     7201 atggtgagcg tcgggttcgc ctgcccgccg gtcgggaaca ccgagcttcc cgcgacaaaa
     7261 agattcggaa ccccgaacac gcgacagtct gcgtccacga cgcctttttg ctcggaatcg
     7321 gccatgcgcg tggtgccaat gtggtgcgcg acatcgcgca ggtgacgcga ccagtcggtc
     7381 tgcgagtcgc cgagccagaa cgggatactc atgcgggcct tgtacagtcg agccatctcc
     7441 gtggcgacgg tcaacacaag tagttgggtg ctgtgccgat cgagctcggt caggcgccag
     7501 tcggccatga cgcggcgcat gcccagcgca tcggtcgctt ccgcaagccg tattcgactc
     7561 gacgggttcg gtgactgctc accctccagc aggatctgcc gcagcgtgcg cgaatacggc
     7621 tccgcgccgg gcctgaggaa gcgacgccgc acgtttagcg cgacgtctga aaaatccgcg
     7681 acgacattga agatctcgcg gcccgcgtcc ttcggccaat ggccagactt gagttcgtcg
     7741 gccaggcgcg tgagcgaact cgtgccgctg tcggggtcca ggcccccttc tcgataggtc
     7801 gtcacggagc cgttcaacac cttgctggct tcctggaagc gatcgcagag gcgtacgccg
     7861 accacgtagt ttccgaagga ggagtcgtgt cgcgcgtaca tgcgcgagac gaggtgagga
     7921 tcgccctcca tcaccagcgt cgcatcgacg cgcaggtgtt ccatgaaaaa tcggcccacg
     7981 aggtcgcgct ggttgccgac gcccgttttc tgcacgtcgt ccgacatgag gagcaaacgt
     8041 ggattttcga tgccgccggc ggcgaggacg tagtagcgcg ccttgacgcg atgacgcgtg
     8101 ccctcggggg tcgaaagggc gatgtgctcg acctgcgtgc cctctggatt cagcgccacg
     8161 ctggtcgcgg tcgcgtgcaa gaccacctgg atgttctcca ccacacgcag cactgcaccg
     8221 tacttgccgg caaagcgggt tgggccgctc atctgccagc attgcggctc gagcttgtcg
     8281 cgatccagtt gcatgtccgg cacgccaagc gcttcgagca catcttcatc gtagaagtag
     8341 ttgccgaggt cgcagatgcc ctgggcgcgc tcgtaaaagg ggtcgaggtc ggcgcgcgtg
     8401 atgggccagc cgctgtgcgg cacccagctg cgcgcggcga aatccacggc gtcgagcgga
     8461 gcgcatcgcc ctgtccagtg gttggtggtg ccgccgaaca tgcgcagacg cgactgcatg
     8521 ccttgcaccg ggacctggcc ctcgttgtcg acgtggtaaa gggcctggct gtcggcctcc
     8581 atgtgctcgc cgccgccttc gagcaggcac acgcgcgttt tcgtaccgtg gaactgcatt
     8641 gccatgctga tgcccgcggc gccgccgccg atgatgcaga catccgcttc cagcacgtgg
     8701 ccggccggga acgagcgcag gtccgagatc aagcggtatc gcccaggaaa accgcgagcg
     8761 cgcaccagcg tgcttcggtc acggccatga gccagccgcg cacggcgagc acacgacccg
     8821 cgccgaagtc cgcgtcgcgc gaggccgcga ggcgtgacct gagctcacgc gtgttggccg
     8881 gaaggtttgc gccaaacagc gcctgcgcca gggtttcgag gtcggcctcg tgtggcatcg
     8941 cctgcagata ggaacggccg atggcgcgcg cgtcttcgat cgaatggtag gcgccgcgca
     9001 gcaagtcgtt ggtggcgttt tgcggcctcg ccggaacggc gatcgccacc agcgccggcg
     9061 cgaggaggct ggccagcccc gaacggatca gtgagcgccg tgaccagggg tcgcggcaat
     9121 cctcggatcc ggggttggta tcgatgatgg gcatgatttc cggccgctcg cgcgatgact
     9181 ggcgatcctg cgcacgactt tccactccgt caagccgccg cagactgcag tccctaacat
     9241 atcgcgaccg gacccgcgcg gaccacccgg ttgtcgagcg cgccgaatat gctgtggccg
     9301 cgggcgtcga gcatctcgat ggacacgcga tcgccgaact gcaggaacgg cgtgcgcggc
     9361 gcgccaagct ccagctgctc gagcgtgcgc ttc
//
LOCUS       DASQJS010000037        16426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4421284, whole genome shotgun sequence.
ACCESSION   DASQJS010000037 DASQJS010000000
VERSION     DASQJS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16426
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4421284"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VEW08_01510"
     CDS             <1..550
                     /locus_tag="VEW08_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39432.1"
                     /translation="AHAAAALSFERGARSVLVGGGWPASSLPARACADSEFHWDGVRF
                     QTYAAGNGGRYCALRVSVGAHAVLLGGDLDAAAERSLIARVERGALASDAVLVSRHGS
                     PVASAPEWIEASAASLAIATGGIVNSHSRDVTLARWRASGARVLDTRRDGGVELGLGT
                     SGLEVLGTVRASKYPFVWRRSP"
     gene            587..1231
                     /locus_tag="VEW08_01515"
     CDS             587..1231
                     /locus_tag="VEW08_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYJ39433.1"
                     /translation="MWELVRAGGPFMWPIILCSIALVGIVLERLWTLQRKRVLPQELI
                     KKVSELAERNQVNPKVIEALEKNSALGRVLAAALANRHRGREIMMERVEDVGRQVVHE
                     LERFVNSVGTIASISPLLGLLGTVTGIIEAFNAVMLGGMGDPRLLAGGISEALLCTAA
                     GLTVAIPAYVAHRYLRGKVERIVVEMEKIAVNFADSLGADDQSGDSDGGVARSA"
     gene            1234..1659
                     /locus_tag="VEW08_01520"
     CDS             1234..1659
                     /locus_tag="VEW08_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYJ39434.1"
                     /translation="MQSRKGKEDPEINLIPLIDVALLLVIFFMLSSTFMQEGRLKIEL
                     PQASLAPTGKQKVDPIVVSVTQSGSYRVNDRELINSSPDTLRAAILEVAGADRDKPVT
                     VRADGRATHQSVVSAMDVLGKLGFVRLNIATVEDSSPKK"
     gene            1656..3428
                     /gene="msbA"
                     /locus_tag="VEW08_01525"
     CDS             1656..3428
                     /gene="msbA"
                     /locus_tag="VEW08_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HYJ39435.1"
                     /translation="MNPTPPPDASAAKVYRRLLAYARPHRGMFMIGVVGMVLFSATDG
                     AFVYFVQKFIKGMTDLQFESVTAANPMIFWLIPIGAPVLFLLRGVGDYMSTYFPGWVG
                     RQVIKSIRGDLFRQYLHLPASYYDRASSGMLLSKLTYNIEQVAEATTKSITSIIRDTL
                     TIIVLIGMMFYNSWQLAAFVFLIAPPLSWLVRKVSSSFRRYSARIQASMGDVTRVVKE
                     ALDGQRVIKVFNAQDQEAADFEKVNEHNRRSNMKLIAARAISNPVVQFIASLALGGIL
                     WVAMRQIAKGTLALDTFMGMLTALLMIPQPLKRLMDSFVPLAQGVAAGASVFEVLDTP
                     SEDFKSGRPLGRVSGAVEFRDVRFEYSTEKGVVLNSVSLNVPAGKTVAIVGPSGGGKS
                     TLVALVPRIYDVNSGEVLVDGVNVHDCNLRDLRANVALVSQDVLLFNDSIRNNIAFGV
                     ENPDPKAVEAAAKAAYVDEFAATLPQGLDTEVGERGSQLSGGQKQRVAIARALLKNAP
                     ILILDEATSALDNESERRIQQALVELMRNRTTLVIAHRLTTIEHADEIVVMVAGNIVE
                     RGTHADLSARSGVYASLRNAEFRN"
     gene            3425..4456
                     /gene="lpxK"
                     /locus_tag="VEW08_01530"
     CDS             3425..4456
                     /gene="lpxK"
                     /locus_tag="VEW08_01530"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HYJ39436.1"
                     /translation="MRSSIEAMWYGGKGPLWLAPLSFLYGGIMGFRSLLYRMGLRHRV
                     RVKAPVIVVGNLTVGGTGKTPLVAWLSTHLAAVGLRVAIVSRGYGGRARGVTRVTVHS
                     RPSEVGDEPLLLARRSQATVFVGKDRVAAAKAAVADGADIVICDDGLQHLALMRDCEI
                     VVIDGQRGFGNGCLLPRGPLRELPRRLRRVNAVVVNGAITAPGFSLPGFLARTHYVMN
                     MRPGDARPVSGGGSLRALSSFRGTGVHAVAAIGNPQRFFDMLRSAGLTLYEHPMPDHH
                     AFKAGDLNFGDNLPVIMTEKDAVKCVAFADDRCWFVPVTAEFTENETRELTDLVLARA
                     SEFKAKANG"
     gene            4449..4637
                     /locus_tag="VEW08_01535"
     CDS             4449..4637
                     /locus_tag="VEW08_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HYJ39437.1"
                     /translation="MDERLLDILACPICKGPLKFQRAAEVLVCRAERLAFPMRDGVPM
                     MLEEDARQLTPDDPLLER"
     gene            4644..5390
                     /gene="kdsB"
                     /locus_tag="VEW08_01540"
     CDS             4644..5390
                     /gene="kdsB"
                     /locus_tag="VEW08_01540"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HYJ39438.1"
                     /translation="MNFRVVIPARFDSSRLPGKALLPLAGKPMLQWVHERARDSAATE
                     VIVATDDERIAVAARAFGAEVAMTARTHVSGTDRIAEVAAARGWADADIVVNVQGDEP
                     LIPPRVIDQVAELLRANARADIATLAVKIESPNDLADPNNVKVACDSGGRALYFSRAP
                     IPFNRDAPATLSSACLRHVGIYAYRVSALRRLASLPAARLELIEKLEQLRALENGMEI
                     RVALAVERPLADVNTAADLERAERALSLRP"
     gene            5400..6215
                     /locus_tag="VEW08_01545"
     CDS             5400..6215
                     /locus_tag="VEW08_01545"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HYJ39439.1"
                     /translation="MNVTLTDLHVYPIKSCRGIALESARLAPTGLTDDRHWMLVRPNG
                     RFITQREYPRMALLSTQVNDDELILGAPYMANLKVARAAGGESRAVTVWKFDGRGIDC
                     GDAAAAWVSQFLETPLRLVMFDVSAPRVCSPEWTGETRAITEFSDGYPILVISRASLA
                     ELNSRLPKALPMERFRPNVVIDGVDAYDEDRIHELRAGPVTLRIVKPCTRCSITTTDQ
                     QRGAVDGVEPLQTLKEYRFDKQLRGVAFGQNVIIVSGVGETLRVGQTFDVTWK"
     gene            complement(6231..8117)
                     /locus_tag="VEW08_01550"
     CDS             complement(6231..8117)
                     /locus_tag="VEW08_01550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HYJ39440.1"
                     /translation="MALAVCSLPQAHAACVGTQGGDLADVEERAFRDPATALPELANT
                     ITSTADMPPARRAALHAIAAEAARQLGFSRQSITHADAGLALLPAGDMSDLAVRMRTV
                     RAVVSTNVGGIDAATVELTRIVDAVRDRPLALGCVLRDRGWLNFRDGNPDQALDDLLR
                     SYALLRRNASREEAMVAAGRLSMAQFSVRDYPQALALVDETITFFREQKAQIRLATAL
                     DRRARILKAAGRLDEAMIAATESLRIHESIGDRVGTGLSQMTMCNVQIERNALTQAAT
                     WCDRAELTLSQTSGMDDNDYRTLAALRGRLYLALGRSRDAVAQFDRAIAPGGAQPAYD
                     IAELYELRSRAHAAVGDYPAAFTDQGEYLRRMREQGTLDRIREVAQLRVQFENDQEQQ
                     KIALLQKDKKLAEERLKSQTRKTQVVAIAGLTGLVTAFFLGYALLSHRRHRAELIRLA
                     ERDDLTGLLNRRAIVRKAVEFLTRARESKGTLIIGLIDLDHFKSINDRFGHAVGDQLL
                     QRFAAALRTAMHTSEVFGRYGGEEFLVLFPDSSLDQARQSADRLRGALRDQRMRVDDQ
                     DVSVTLSLGLASYEAGDVLFDQVARRADIALYVAKTQGRDRVEVFNPARHGTLAATAA
                     QRQR"
     gene            complement(8200..8841)
                     /locus_tag="VEW08_01555"
     CDS             complement(8200..8841)
                     /locus_tag="VEW08_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39441.1"
                     /translation="MRTDNFDRTIRAAAGIALLALALTGCDKPAPQVAPPEPPVQPAP
                     APAIPDAPPPDPAPAPAPDAPPPTEPSAVPKPASSNEPLVDSMLAAIPSTKMSVAVDL
                     RYSFDGEVLPNQPVIVHLAAVPRVGGANMRVSVQQAAGLQMAAGPLTVQKANASGVYR
                     QQFSLTRLAGTPEPLRVLVTMQMGEDTGFGYFTIPLPPDGGKSAQKQESVKQR"
     gene            complement(8850..10811)
                     /locus_tag="VEW08_01560"
     CDS             complement(8850..10811)
                     /locus_tag="VEW08_01560"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HYJ39442.1"
                     /translation="MRHPTPGLTLAGIAALLLVACSGGGGGSGSSSGGNPANTAPVAN
                     AGASQTVTSGTTVTLNGTASSDSDGTIASYAWTQTAGTAVALSSATASQPTFPAPTVA
                     SATALTFSLVVTDNRGTASSASTVTVTVNPPAGGNGTITGQITFGRVPFATQLQDPNR
                     GLWYATPIQQPARGVIVRAVDPSSPTTVLATGSTDNLGNYSLSVAANTTVTLEVVAQM
                     LRDASQPLPRWNVRVQDSTTGTSPYTFIEPGSFNSSAGIAHNVAIPTGISAAGVATGT
                     RASAPFAILDTVYQGIQSVIGVAPTTNFPALLVDWGSQTDGTFFSPGSTQRMALLADL
                     STDTDEFDQHVIAHEFGHYVEFNFSRADNIGGSHGLGDKLDPRVAFGEGFGYAFAAIV
                     LNDPVARDSANDNGDLWSGVFRIEDNPQTSMAGAPFDNYGCWCSESSVYSLLWDLYDN
                     TADANDTVALGFAPIWEVLTGAQRTTPAHTTIFSFITALKAARPGDAGAINTLVAAQN
                     IDATNIDAFATGETHVPTNVSAADVLPLYTTITASGSPVVVRNVNDAGVENKLGNHRF
                     LRFTPNITGSVLVTLNSSNTNNADPDFRMQRAGTFVLVAEDGPPQPQTGTVSVVAGTT
                     YVLDVHDCANGCAGMQGVAGDYDLTVTIN"
     gene            11018..13501
                     /locus_tag="VEW08_01565"
     CDS             11018..13501
                     /locus_tag="VEW08_01565"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HYJ39443.1"
                     /translation="MALIFLSVIAAVFVGVVALEGGDPSLERRPKGLLTWIASGNWPA
                     KIGGALIVVGVGALLRYAAINFDVPPAFKLALGIGASAALGFASFLTAANTHRRVISL
                     AFGGAAFGVAYLTAYSAFALFGYLPTMQGLGLLVLTALGAGVFAVSRSALSLAVLSMV
                     GAFIAPAFAVDDPGPRVVYGYYVAASLLTLGMVSFRGWRPLIHLSFLFTLAGSAFFAW
                     TADYFAPGNSTVIFPLLAMLVAVHVAMPIAERRWARGKVVESLDTVYLLALPVVAALT
                     AQALAPSRAMLAVQLWWYAGIWLAASAWLYSQRREGMASHAIIGLLMLGCGLAARFRD
                     LPWELVGLGIAVISLVLAARRSQSTRLQGFLAGLVLVLAVIHMINALAPATDSTVFLN
                     QRFVERVIGAGLLFIAALTLTRLRHTLDSLMLTGAIGWAAFAVGAEIVRLDLVSAWLV
                     VHWLLVFAGFAAYFAAARVTAVERLIVPLVLAIGLSGLLAQMRTSAELSWVSAFVSGL
                     SLLAIALRPAGENGGATSGRPLAAIGAPILVALWVGRVAHLASEHWQFPLAVAAAAAI
                     LVLLVGYLAKERSNQWFNEATDIFAMAFTFVLAAAAIFDIERVASATVLELLCVGGLV
                     LIANWNDNRVRVGHWIIPATVIGVALVLQANLLRWLGPPGHLNALDVTKMDWPTLVSL
                     LWASIGAALTIWARRIGSRVLWSGGAVFLVSAAVKLLLLDFGSLGQLANILAVIAAGG
                     VFMLVGWLAPMPPAPEKPQEKPSVKSPATPAQPAGTLYAEWQLQQRARREGPAVEKVK
                     DDDMSGKFAWTVTIAALVLITYSQCDGRL"
     gene            13542..14009
                     /locus_tag="VEW08_01570"
     CDS             13542..14009
                     /locus_tag="VEW08_01570"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HYJ39444.1"
                     /translation="MKILFVCLGNICRSPTAEGVLRAIAAHEFPGLKLDIDSAGTADY
                     HVGEPPDRRTVAAARRRGYDLAGLRARQVQSGDFSRFDYVLAMDRANLVELQRLRPQG
                     AATRLALFLEFAPDVSELEVPDPYYGGVADFERVLDLCEMAARGLLARISSGD"
     gene            complement(14080..>16426)
                     /locus_tag="VEW08_01575"
     CDS             complement(14080..>16426)
                     /locus_tag="VEW08_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HYJ39445.1"
                     /translation="AALTTFISLAGRFLVLMPNNPRAGGVSRRIEGEDRDQMRQVMDE
                     LVIPDGMGAIVRTAGVGRAAAELQWDLDNLKSQWDQIDAATKDRPAPYLVYRESDAVT
                     RSLRDYLADDIGEVLVDDDATFATAQEYMTRFMPPEAQKRLKRYTDDVPLFTRFQIEN
                     QIESAYAHKVELPSGGSIVVDYTEALVSIDINSARATRGSDIETTATNTNLEAADEIA
                     RQLRIRDIGGLIVIDFIDMESPKNQRDVEDRLRDAVKMDRARIQIGRLSRFGLLEMSR
                     QRLRPSLGDSSHLVCPRCTGIGSIRSVESMTLAILRLIGEEARKDKTAQIVAQVPVEV
                     ATYLMNEKREWMRTLEDKSSVDLMIVPNPHIQTPEYSIKRVRTDEIDLPENKVLSYKM
                     PERPQVADPTGTRDIKPVLEPAAVIPTAPATAAPVVVHVPVPAKEVAVAVKTHPANLG
                     FWSRLVYLFTGSAPAAKAAEPAPARSEPRRDGHRDGHRDRDRGRPRHGGGDRDRGRDR
                     DRDRGAHGRDRNRDKEGGHRRDKEGGNRDRHREHGRDRDNNRDASQPPREASQPPRDA
                     QREPQREGQREQAPRERDPQREAERQAQRERQRERQREQQAAASANAAAAPSMPAGEN
                     AGANEPPPPILEGGNGPAQDGPRGDRAPRRGRRRGRRGGGRNRDGGVQPGNMGADGNE
                     GTNFAGAREASDEEREFHAQMTNGNRGEGRDQGHGESSASDRPEPQREPPREPQREPQ
                     RESQNEHSQAPLDLPPPPPTKPFVVWSSSPSDAVPGPRRDD"
BASE COUNT         2544 a         5187 c         5599 g         3096 t
ORIGIN      
        1 cgcgcatgcc gcggcggcgc tgtcattcga acgcggggcg cgatccgtgc tggtcggcgg
       61 cggctggccg gcaagttcgc tgccggcgag agcttgcgcg gattcggagt tccattggga
      121 tggcgtccgc ttccagacct acgcggccgg caatggcggg cgatactgcg cgctgcgcgt
      181 ctcggtgggc gcgcatgccg tactgctagg cggcgacctc gatgccgcgg cggaacgctc
      241 gctgatcgcg cgcgtggaac gcggcgcgtt ggcaagcgac gcggttctcg tgagccgcca
      301 cggcagtccg gtggcatcgg cgccggagtg gatagaagcg agcgccgcga gcctggcaat
      361 cgctaccggc ggtatcgtga attcacactc ccgcgacgtg acgttggcgc gctggcgcgc
      421 atccggcgcc agggtgctcg acactcgtcg cgatggcggc gtcgagctcg gcttggggac
      481 gtcgggcctg gaggtgctcg gcacggttcg cgcctcgaaa taccctttcg tctggcggcg
      541 ttcgccgtga ccctttcgaa cccgccctcc ggtatagtct ccgcccatgt gggaactcgt
      601 acgggccggc ggcccattca tgtggccgat catcctttgt tcgatcgcgc tcgtcggcat
      661 cgttctcgaa aggctctgga ccttgcagcg caaacgcgtg ctgccccagg agctcatcaa
      721 gaaggtgtcc gagctcgccg agcggaacca ggtcaacccg aaggtcatcg aagcactcga
      781 aaagaactcg gcgctcgggc gtgttctcgc ggcggcgctg gccaaccgcc accgcggtcg
      841 cgagatcatg atggaacgcg tcgaagacgt gggccgccag gtggttcacg agctggagcg
      901 gttcgtgaat tccgtgggca ccatcgccag catttcgccg ttgttagggc tgctcggtac
      961 cgtgaccggc atcatcgaag cgttcaatgc ggtgatgctg ggcggcatgg gtgatccgcg
     1021 cctgctggcg ggtggtatct ccgaagcact gctctgcacc gcggctggcc tcacggtggc
     1081 gattcctgcc tacgtcgcgc accgttacct gcgcggcaag gtcgagcgca tcgtcgtcga
     1141 gatggagaaa atcgcggtga attttgccga ctccctgggt gccgacgatc aaagcgggga
     1201 cagcgacggt ggcgtcgctc gcagtgcatg aggatgcagt cccgcaaggg gaaggaagat
     1261 cccgagatca acctgatccc gttgatcgac gtggcactgc tgctggtcat cttcttcatg
     1321 ttgtcgtcca cgttcatgca ggaagggcgg ctcaagatcg agctgcccca ggcgagcctt
     1381 gcaccaacgg gcaagcagaa ggtcgacccg atagtcgtct ccgtgaccca gagcggcagc
     1441 taccgcgtga atgaccgcga gctcatcaac tcgtcgccgg acacgctgcg cgcggccatc
     1501 ctggaagttg cgggcgccga tcgcgacaaa cccgtcaccg tacgtgccga tggacgggcc
     1561 acacaccagt cggtggtaag cgccatggac gtgctcggca agctcggttt cgtgcgcctc
     1621 aacatcgcga cggtcgaaga ctccagcccc aagaaatgaa cccgacaccg ccgccggacg
     1681 ccagcgccgc gaaggtctat cgccggctgc tcgcatacgc gcggccccac cgcggcatgt
     1741 tcatgatcgg cgtcgtcggc atggtgctgt tctcggccac cgatggcgcc ttcgtgtact
     1801 tcgtgcagaa gttcatcaag ggcatgaccg acctgcagtt cgagtcggtc actgcggcga
     1861 atccgatgat cttctggctg atccccattg gcgccccggt gctgttcctg ctgcgcggcg
     1921 tcggcgacta catgtccacc tattttcccg gatgggtcgg ccggcaggtc atcaagagca
     1981 ttcgtggtga cctgttccgc cagtatttgc acctgccggc gagttattac gaccgggcgt
     2041 cgagcggcat gctgttgtca aagctcacgt acaacatcga gcaggtggcc gaggccacca
     2101 ccaagtcgat cacctcgatc atccgcgaca ctctgacgat catcgtgctg atcggcatga
     2161 tgttctacaa cagctggcaa cttgccgcgt tcgtgttcct catcgcgccg ccgctgtcgt
     2221 ggctggtgcg caaggtgtcg agctcgttcc gccgttacag cgcgcgcatc caggcgtcga
     2281 tgggtgacgt gacgcgggtc gtcaaggaag cgctcgatgg ccagcgcgtc atcaaggtct
     2341 tcaacgcgca ggaccaggaa gccgccgatt tcgagaaggt caatgaacac aaccgcagga
     2401 gcaacatgaa gctcattgcg gcgcgcgcca tcagtaatcc cgtggtgcag ttcatcgcct
     2461 cactggcact cggcggcatt ctctgggtcg cgatgcgcca gattgccaag ggcacgctgg
     2521 ccctggatac gttcatgggt atgctcaccg cgttgctcat gattccgcag ccgctgaagc
     2581 ggttgatgga ctccttcgta ccgctcgcgc agggcgtcgc tgccggcgcc agcgtgttcg
     2641 aggtgctcga caccccctcg gaggacttca aatccggtcg acctctcggt cgcgtctcgg
     2701 gcgccgtgga atttcgtgac gtccgtttcg agtactcgac cgagaagggt gtggtactga
     2761 attccgtgtc gctcaacgtg cccgccggca agacggtggc cattgtcggg ccgtccggcg
     2821 gcggcaagtc cacgctggtc gcgctcgtgc cgcgcatcta cgacgtgaat tccggcgaag
     2881 ttctcgtcga tggcgtcaac gttcacgact gcaatctgcg tgacctgcgc gccaacgtcg
     2941 cgctcgtgag ccaggacgtg ctgctgttca acgactccat tcgcaacaac atcgcgttcg
     3001 gcgtcgaaaa tccggatccg aaggccgtgg aggcggcggc caaggccgcg tacgtcgacg
     3061 aattcgctgc cacgctaccc caggggctcg acactgaagt cggggagcgc ggttcgcaat
     3121 tgtctggtgg ccagaagcag cgcgtcgcga tcgcgcgcgc gttgctcaag aacgcgccca
     3181 tcctcattct tgacgaagcc acgtcagcgc tcgacaacga atccgagcgc cgcatccagc
     3241 aggcgctcgt cgaactcatg cgaaatcgca ccacgctcgt catcgcccat cggctcacga
     3301 ccatcgaaca cgccgatgag atcgtggtca tggtcgcggg caacatcgtc gagcgcggca
     3361 cccatgccga cctttcggcg cgcagcggtg tgtatgcctc tctgcgcaac gcggaattcc
     3421 gcaattgaga tcaagcatcg aagccatgtg gtacggcggc aaggggccgc tgtggctggc
     3481 accgctctcc ttcttatatg gcggcatcat gggctttcgc agcctgctgt accgcatggg
     3541 gttgcggcac cgggtgcgcg tcaaggcgcc ggtcatcgtc gtgggtaatc tcaccgtcgg
     3601 cggcacgggc aagacgccgc tggtcgcctg gctttcgacg catctcgccg ccgtgggcct
     3661 gcgcgtggcc atcgtgtcgc gcggttatgg tgggcgtgcc cgcggcgtta cgcgagttac
     3721 ggttcacagc cgtccgtccg aagtagggga cgagccgctg ctgctggcgc gccgctcgca
     3781 ggcaacggtg ttcgtcggaa aggatcgcgt tgccgctgca aaagcggcgg tagccgacgg
     3841 ggccgacatc gtcatctgtg atgacgggct tcagcatctg gcgctgatgc gcgactgcga
     3901 aatcgtggtc atcgacgggc aacgaggttt cggcaatggc tgcctgctgc cgcgtggacc
     3961 gctgcgtgag ctgccgcggc gtttgcgccg cgtaaatgcg gtggtggtca acggcgccat
     4021 caccgcgccg ggtttttccc tgccgggctt cctggcgcgc acgcactacg tcatgaacat
     4081 gcggcccggc gacgcgcgcc cggtttccgg gggcggctcg ttgcgcgcgc tttcgagttt
     4141 tcgcggcacc ggcgtacacg cggtggcggc catcggcaat ccgcagcgct tcttcgacat
     4201 gctgcgaagc gcgggattga cgctgtacga acatccgatg cccgatcacc acgcgttcaa
     4261 ggcgggcgat ctgaattttg gcgacaacct gccggtcatc atgacggaga aggatgccgt
     4321 aaaatgcgtc gccttcgccg acgaccgctg ctggttcgtg ccggtcaccg ccgaatttac
     4381 ggagaacgaa acgcgcgaat tgacggatct cgtcctggcg cgagccagcg agttcaaggc
     4441 caaagcgaat ggatgaaaga ctgctcgaca tactcgcgtg cccgatctgc aaggggccgc
     4501 tgaagtttca gcgcgccgcg gaggtgctgg tgtgccgcgc cgagcgactg gcgtttccga
     4561 tgcgcgatgg agtgcccatg atgctcgaag aagacgcacg ccagctcacg cccgacgatc
     4621 ccctgctgga gcgctaggtg accatgaatt ttcgtgtcgt cattcccgcg cgtttcgatt
     4681 catcgaggct gccgggcaag gcattgctgc cgctcgccgg caagccgatg ctgcagtggg
     4741 ttcacgaacg cgcgcgagac agcgctgcga ccgaggtcat cgtcgccacc gatgacgaac
     4801 gcatcgcggt cgccgcgcgc gcattcggtg cggaagtcgc catgaccgcc cgcacgcatg
     4861 tttcgggcac ggatcgcatt gccgaagtgg cggcggcgcg cggctgggcc gacgccgaca
     4921 tcgtcgtgaa cgtgcagggc gacgagccgc tgattccgcc gcgcgtcatc gaccaggtgg
     4981 cggagcttct gcgtgcgaat gcgcgcgccg acatcgccac gctggcggtg aagatcgaat
     5041 cgccgaacga tctcgccgac cctaacaacg tgaaggtggc ctgcgattcc ggcggccgtg
     5101 cgctgtattt ctcgcgcgcg ccgattccct tcaatcgcga tgcgccggcc acgctgtcca
     5161 gcgcctgcct gcggcacgtc ggtatctatg cctaccgcgt gtcggcgctg cgccggctgg
     5221 cgagcctgcc ggctgcgcgc ctggaactca tcgagaaact cgaacaattg cgcgcgctcg
     5281 aaaatggcat ggagattcgc gtggcgctgg ccgtggaacg gccgctggcc gacgtgaata
     5341 ccgctgctga tctcgaacgt gccgagcgcg cgttgtcgct gcgcccttga gaactgatct
     5401 tgaacgtcac actgaccgac ctgcacgtct acccgatcaa gtcctgccgc ggcattgcgc
     5461 tcgagagcgc ccggctcgcg cccaccggac tcaccgacga ccggcactgg atgctggtgc
     5521 gtcccaacgg caggttcatc acgcagcggg aatacccccg catggcgctg ctctcgacgc
     5581 aggtgaacga cgacgagctg atcctgggcg cgccatacat ggcgaatctc aaggttgcgc
     5641 gcgccgccgg gggcgagtcg cgcgcggtga cggtgtggaa attcgatggc cgcggcatcg
     5701 attgcggtga cgccgctgcg gcgtgggtgt cgcagttcct ggagacgccg ctgcgcctgg
     5761 tgatgttcga tgtcagcgcg ccgcgagtct gcagccccga gtggactggc gaaacgcgcg
     5821 cgatcaccga gttcagcgac ggctatccga tcctggtgat ttcgcgcgcg tcgctggccg
     5881 agctcaattc acggttgccc aaggcgctgc cgatggagcg tttccggccg aacgtggtca
     5941 tcgacggtgt cgacgcatac gatgaggacc gcatccacga gctgcgagcg ggcccagtca
     6001 ccctgcgcat cgtgaagccg tgcacgcgat gctccatcac caccacggat cagcaacggg
     6061 gcgcggtcga tggcgtcgaa cctctgcaga cgctcaagga ataccgcttc gacaagcaat
     6121 tgcgcggcgt ggcgttcggg cagaacgtca tcatcgtaag cggtgtcggg gagacgctgc
     6181 gggtggggca gaccttcgac gtcacgtgga aatagggacc tgtccccatt tcaacgctgg
     6241 cgttgggcgg cggttgcggc gagggttccg tgccgcgcgg gattgaagac ctcgacgcga
     6301 tcacggccct gggtcttcgc gacatacagc gcgatgtcgg cacggcgcgc cacctgatcg
     6361 aacaacacgt cgccggcttc ataacttgcg agcccgaggc tgagcgtaac ggaaacatcc
     6421 tgatcatcga cccgcatgcg ctggtcacgc agcgcgccac gcaggcgatc ggccgattgc
     6481 cgcgcctgat cgagtgagct gtcggggaac aacacgagga attcctcgcc gccgtagcgg
     6541 ccgaagactt cgctcgtgtg catcgccgtc cgcagcgctg cggcgaaacg ctgcagcagc
     6601 tggtcaccga ccgcgtgccc gaaacggtca ttgatcgact tgaagtggtc gagatcgatg
     6661 agcccgatga tgagtgtgcc cttcgattcc cgcgcgcgcg tcagaaattc cacggccttg
     6721 cgcacgatgg cgcggcgatt caacaggccg gtcaggtcgt ctcgctcggc gagccggatg
     6781 agctcggcgc gatgccgccg atgcgagagc agggcgtagc ccaggaagaa cgccgtcacc
     6841 agtcccgtga gtccggcgat ggcgacgacc tgcgtcttgc gcgtctgact cttgagccgc
     6901 tcttccgcga gcttcttgtc tttctggagc agcgcgatct tctgctgttc ctggtcgttc
     6961 tcgaactgca cgcgcaattg cgcgacttcg cggatgcgat cgagcgtgcc ctgttcgcgc
     7021 atgcggcgca ggtattcgcc ctggtcggtg aacgcggccg gatagtctcc cacggcggca
     7081 tgggcgcgcg aacgcagctc gtagagttcg gcgatgtcat aggccggctg cgcgccgccg
     7141 ggcgcgatgg cgcggtcaaa ctgcgccacg gcgtccctgg aacgccccag cgcgagatac
     7201 aggcggccgc gaagggcggc cagggtgcga tagtcgttgt cgtccatgcc gctcgtctgg
     7261 ctgagcgtga gttcagcgcg gtcgcaccag gttgccgcct gggtcagtgc gttgcgctcg
     7321 atctgcacgt tgcacatggt catctgcgac aggcccgtgc caacacggtc gccaatgctt
     7381 tcatgaatac gcagcgattc ggtggcggcg atcatcgcct cgtcgaggcg gccggcagcc
     7441 ttgaggatgc gggcgcggcg gtcgagcgcg gtggcgagtc ggatctgcgc cttctgttcg
     7501 cggaaaaagg tgatggtttc atccaccagc gcgagcgcct gcggatagtc gcgcacgctg
     7561 aactgcgcca tcgagagtcg gcccgcggcg accatggctt cttcgcggct cgcgttccgc
     7621 cgcagcagcg cgtacgaacg caacaggtca tcgagcgcct ggtcgggatt gccgtcccgg
     7681 aaattcagcc agcctcgatc gcgcagcacg cagccgagcg cgagcggccg gtcgcgcacc
     7741 gcatcgacga tgcgcgtgag ttcgacggtg gcggcgtcga tgccgccgac attcgtgctg
     7801 accacggcgc gcacggtgcg catgcggaca gcgagatccg acatgtcacc cgctggcagc
     7861 agcgcgaggc cggcgtccgc gtgcgtgatg ctctggcggc tgaagccgag ctggcgcgcg
     7921 gcctccgcgg cgatggcatg taacgccgcg cgccgcgcgg gcggcatgtc ggcggttgag
     7981 gtgatggtgt tcgcgagttc cggcagggcc gtggccgggt cgcggaaggc acgttcttcg
     8041 acgtcggcga gatcgccgcc ctgagtgccg acacaggcgg catgagcctg cggcaacgag
     8101 cagacggcca gtgccatcag gcagctgact cgcagcctta tctccctggt gttcgagcaa
     8161 atcacggcgc gtagtctacg cgcccgcgct gcagtgcggt cagcgctgtt ttacggattc
     8221 ctgtttttga gcactttttc caccgtcggg cggtagcggt atcgtgaaat acccgaatcc
     8281 cgtgtcctca cccatttgca tggtcaccag gacgcgtaat ggctcgggag tgcctgcgag
     8341 tctggtcagc gaaaattgct ggcgatacac gcccgacgcg ttcgctttct gcacggtcag
     8401 cgggcccgct gccatctgca gtccggcggc ctgttgaacg ctcaccctca tgttggcgcc
     8461 ccccacgcgc ggcacggcgg ccagatgcac gatgaccggc tggttcggaa gtacctcacc
     8521 atcaaagctg taccggaggt ccacggcgac gctcatcttg gtgctgggaa tcgcggcgag
     8581 catggaatca accagcggtt cgttggacga cgccggtttc ggaaccgccg agggttcggt
     8641 gggcggcgga gcgtccggtg ccggtgccgg tgcggggtcg ggcggcggcg catcgggaat
     8701 cgcaggtgca ggggcgggct gcaccggtgg ttccggcggc gcaacctgcg gcgccggctt
     8761 gtcgcaaccc gtgagcgcca gggctaacag ggcgatcccg gctgcggcgc gaatcgttct
     8821 gtcgaagttg tcagtacgca tgtaacgcct cagttgatcg tgaccgtgag gtcgtagtcg
     8881 ccggccacgc cctgcatgcc ggcgcagccg ttggcgcaat cgtgcacgtc gagaacatag
     8941 gtagtgccag cgaccacgga gaccgtgccg gtctgcggtt gcggtggacc atcctccgcg
     9001 acgagcacaa acgtgccggc acgctgcatc cggaaatccg ggtcggcatt gttcgtattc
     9061 gacgaattca gggtcacgag gaccgagcca gtaatgtttg gtgtgaagcg caggaagcgg
     9121 tgattgccga gtttgttctc gacgccggca tcatttacgt tgcgcacgac cactggagag
     9181 ccactggccg tgatggtcgt gtatagcggc agcacatcgg cggccgagac gttggtgggt
     9241 acgtgggttt caccggtcgc gaacgcgtca atgttggtgg cgtcaatatt ctgcgcagcg
     9301 acgagtgtgt tgattgcgcc cgcgtcgccc gggcgcgcgg ctttcagcgc cgtgatgaaa
     9361 gagaagatgg tggtgtgggc cggcgtagtt ctttgcgcgc cagtcaatac ctcccagatc
     9421 ggcgcgaatc ccagcgcgac ggtgtcgttc gcatcggcgg tattgtcgta caggtcccaa
     9481 agcagggaat agaccgaaga ctcactgcac cagcagccgt agttgtcgaa gggcgcgccc
     9541 gccatgctcg tctgcgggtt gtcctcgatt ctgaatacgc cagaccagag atcgccgttg
     9601 tcgttcgcgc tgtcgcgcgc caccggatca ttgagcacga tggccgcaaa tgcgtagccg
     9661 aacccttcgc cgaatgcgac gcgcggatcg agtttgtcgc cgaggccgtg tgaaccaccg
     9721 atgttgtcgg cgcgcgagaa attgaattcg acgtagtggc cgaactcgtg cgcgatgacg
     9781 tgctggtcga actcatcggt atccgtgctg aggtcggcga gcagcgccat gcgctgcgtg
     9841 gatcctggcg aaaagaatgt tccatcggtc tggctgcccc agtcgacgag gagcgcgggg
     9901 aagttagtgg tgggcgcaac cccgatgact gactggatgc cctggtagac ggtgtcgaga
     9961 atggcgaacg gcgcggacgc gcgcgtaccg gttgccacac ctgcggcgct gattccggtg
    10021 ggaatcgcca cgttgtgcgc gatgccggcg ctggaattga aactgccggg ctcgataaac
    10081 gtatatggag aagtacccgt ggtgctgtcc tgcacacgca cgttccagcg cggcagcggt
    10141 tgtgaggcat cgcgcagcat ctgcgccacc acctcgagcg tgacggtggt gttggccgcc
    10201 acggacagcg agtagttgcc aaggttgtcg gtgctgcccg tggcgagcac ggtcgtcggt
    10261 gagctgggat cgaccgcacg cacgatgacg ccgcgcgctg gctgctggat tggagtcgcg
    10321 taccagaggc cacggttcgg atcctgcagc tgggttgcga acggcacgcg tccgaaggtg
    10381 atttgcccgg taatcgtgcc gttgccgccg gccggcggat tcaccgtgac ggttaccgtg
    10441 gaggccgagc tcgccgtgcc acggttgtca gtcaccacga gcgagaatgt cagcgccgta
    10501 gcgctggcga ccgtcggtgc cggaaaagtg ggttgcgagg cagtggcact gctgagcgcg
    10561 accgcagtgc ctgcggtctg cgtccatgca taactggcga tggtcccatc cgagtcgctg
    10621 cttgcggtgc cattcagcgt caccgtggtg cccgaagtca ctgtctgact cgcgccggca
    10681 ttggcgacgg gcgcggtgtt ggcggggttc cccccgctgc tgctgccact gccgccgcct
    10741 ccgccactgc acgccacgag caggagggcg gctatgccgg cgagtgttag gcctggcgtc
    10801 gggtggcgca taccgtctta taccggcgcg cggtgcgcca ggggagtcaa aagacgtctc
    10861 aatacggcgt cagagccctg agttacatgc gaaatgtggg cgtggtcaca tcgtttgata
    10921 ccgccggtgc cggaattctg aactttcagg atttccccca agagccatgc caaacgtgca
    10981 ccattcgcct cgctaccggc cagaggggaa aaacatcgtg gcgttgattt ttctatccgt
    11041 gattgccgca gtgttcgtgg gagtcgtggc gctcgagggt ggagatccat cgctcgagcg
    11101 gcggccgaag ggcctgctga cctggatcgc gagcggcaac tggccggcca agatcggcgg
    11161 cgctctcatc gtagtcggag tcggcgcgct gctgcgttac gcggcgatca acttcgacgt
    11221 gcctcccgcc ttcaagctcg cgctcggcat cggcgcgagt gccgcgctgg gtttcgcttc
    11281 attcctgacg gcagccaaca cgcatcgccg cgtgatctcg ctcgcgttcg gcggcgcggc
    11341 gttcggtgtc gcctacctta cggcgtacag cgcgtttgcg ctgttcggct atctgcccac
    11401 gatgcagggg ttgggcctgc tggtgttgac ggcgcttggt gccggcgtat tcgccgtgtc
    11461 gcgcagcgcc ctgtcgctcg cggtgttgtc catggtgggg gcgttcatcg cgcccgcgtt
    11521 cgccgtggat gatcccggcc cgcgcgtggt gtacggctac tacgtcgcgg ccagcctgct
    11581 cacgctgggc atggtgtcgt tccgcggctg gcggccgctc attcacctga gcttcctgtt
    11641 cacgctcgcc ggcagcgcgt tcttcgcctg gaccgccgac tatttcgcgc cgggcaattc
    11701 cacggtcata ttcccgctgc tggcgatgct ggtggccgtc catgtggcga tgccgatcgc
    11761 cgagcggcgc tgggcgcgcg gcaaggtggt cgagtctctc gacactgtct atctactggc
    11821 gctgccggtg gtcgcggcgc tgaccgcgca ggcgctggcg ccgtcgcgcg ccatgctcgc
    11881 cgttcagctg tggtggtatg cgggcatctg gctcgcggcg tcggcgtggc tgtacagcca
    11941 gcggcgcgaa ggcatggctt cgcacgccat catcggcctg ctgatgctgg gttgcgggct
    12001 ggccgcgcgc ttccgggatc tgccctggga actcgtcgga ctcggcatcg cggtgatttc
    12061 cctggtgctc gcggcgcgac gctcgcaatc gacgcgattg caggggtttc tcgccggact
    12121 cgtcctggtg ctcgcggtga ttcacatgat caatgcgctg gcgcctgcga ccgacagcac
    12181 ggtgttcctg aaccagcgat tcgtcgagcg cgtcatcggc gcgggattgc tgttcatcgc
    12241 cgcgctgacg ctgacccgcc tgcggcacac gctcgacagt ctcatgctca cgggcgcgat
    12301 cggctgggcg gcgtttgccg tcggtgcgga gatcgtgcgg ctcgatctcg tatcggcgtg
    12361 gctggtcgtc cactggctgc tcgtgttcgc ggggttcgcg gcgtatttcg ccgccgcccg
    12421 tgtgaccgcg gtggagcgat tgatcgtccc gctggtgctg gcgatcggcc tctcgggcct
    12481 gctcgcgcag atgaggactt cggcggagct gtcctgggtg agcgccttcg tctccggcct
    12541 ttcgttgctg gcgattgcac ttcgtccggc cggcgaaaac ggaggcgcga cctctggccg
    12601 gccgctcgcc gcgattggtg caccgatcct cgtcgccctg tgggtgggac gcgtcgcgca
    12661 cctggcttct gaacactggc agtttccgct cgctgtggcc gcggccgccg caatcctcgt
    12721 gttgctggtc ggctatctcg cgaaggagcg ttcgaatcag tggttcaacg aagccaccga
    12781 catctttgcg atggccttta ccttcgtgct ggccgcggcc gccatcttcg acatcgaacg
    12841 cgtggcatcc gccaccgtgc tcgagctgct gtgtgtcggt ggcttggtgc tgatcgcgaa
    12901 ctggaatgac aaccgggtgc gcgtgggcca ctggatcatt ccggccacgg tcatcggcgt
    12961 cgcgctggtg ctgcaggcca acctgctgcg ctggctcggt ccgccggggc atctcaacgc
    13021 gctcgacgtg acgaagatgg actggccgac actcgtgtcg ctgttatggg cgtcgatcgg
    13081 cgccgctctc accatctggg cgcggcgtat cggttcgcgc gtgttgtggt ccgggggcgc
    13141 ggtgttcctg gtcagcgccg cggtcaaact actgctgctg gatttcggtt ctctcggaca
    13201 gctggcgaat atcctggcgg taatcgcggc cggtggcgtg ttcatgctgg tcggctggct
    13261 ggcgcccatg ccccccgcac cggagaagcc acaggaaaag ccgtcggtga aatcaccggc
    13321 gacgccggca caacctgcgg gaaccctgta cgcggaatgg cagttgcagc aacgcgcgcg
    13381 gcgcgagggt ccggctgtcg agaaagtcaa agacgacgac atgtccggca agttcgcctg
    13441 gacggtgacc attgccgcgc tcgtgctgat cacctactcg caatgtgatg ggcgcctgtg
    13501 aatgacgaca acgtggtgga gtccacggct gacatgacgc tgtgaaaatt ctcttcgtct
    13561 gtctcggaaa catttgccga tcaccgaccg ccgagggggt cttgcgcgcc atcgctgcgc
    13621 atgagtttcc aggcctgaag ctcgacatcg attccgccgg taccgccgac taccacgtgg
    13681 gtgagcctcc cgatcgccgc accgtggcgg cggcacgccg gcgcggctat gacctggccg
    13741 gcctgcgcgc gcggcaggtg cagagcggtg atttcagccg gttcgactac gtgctggcga
    13801 tggaccgcgc caatctcgtg gagctgcagc gcctgcggcc ccagggcgcc gccacgcggc
    13861 tcgcgctgtt cctcgagttc gcgcccgatg tgtcggaact ggaagtgcca gacccgtatt
    13921 acggcggcgt agcggacttc gaacgcgtgc tcgacctgtg cgagatggcg gcgcgaggat
    13981 tgctggcgcg catttcgagc ggtgactgat tttccgctga aataaaaaag gcccgcacga
    14041 ttgcgcgtgc gggcctcgtg agttgccttg agcagcgcct tagtcgtcgc ggcgcggtcc
    14101 cgggactgcg tcggatggcg aggacgacca caccacaaat ggcttggtcg gcggcggagg
    14161 cggcaggtcg agcggcgcct gcgagtgttc gttttgcgac tcgcgctgcg gttcgcgctg
    14221 gggctcccgc ggcggctccc gctgcggttc gggccgatcg ctcgcgctgg actcgccatg
    14281 gccctggtca cggccttcgc cgcgattgcc attggtcatc tgcgcgtgga actcacgctc
    14341 ctcgtcggag gcttcgcggg ccccggcgaa attcgtgccc tcattgccgt cggcacccat
    14401 gtttcctggc tggacaccac cgtcccggtt gcgtccacca ccgcgacgtc cgcgacggcg
    14461 gccacgacgt ggcgcacggt caccgcgagg accatcctgt gcggggccat tgccgccctc
    14521 gaggatcggc ggcggcggtt cgtttgcgcc ggcattctcg ccggccggca tgctgggtgc
    14581 cgcggcggca ttggcgctgg cagcggcctg ctgctcgcgc tggcgttcac gctggcgctc
    14641 acgctgagcc tggcgttcgg cctcacgttg cggatcgcgc tcgcgcggcg cctgctcccg
    14701 ctgcccctcg cgttgcggct cgcgctgcgc gtcgcgaggc ggctggctgg cctcgcgtgg
    14761 tggctgactg gcgtcgcgat tgttatcacg atcgcggcca tgttcacggt gacggtcacg
    14821 attgccgcct tccttgtcac gacgatgacc gccttccttg tcgcgattgc ggtcacggcc
    14881 atgcgcgcca cgatcacggt cccgatcacg tccacggtcg cgatcgccac cgccatgccg
    14941 ggggcggccc cggtcacgat cacgatggcc atcgcgatgt ccgtcacggc gcggttcgct
    15001 gcgcgcaggg gccggctcag cggccttggc ggccggggcg gagccggtga atagatagac
    15061 gagccgcgac cagaagccaa gattggcagg atgcgtcttg accgccacgg ccacttcttt
    15121 cgccggaacg ggaacgtgca cgaccaccgg tgcggcggtt gccggcgcag tggggatgac
    15181 ggcggccggt tcgagcaccg gcttgatgtc gcgagtgccg gtgggatcgg ccacctgcgg
    15241 acgctcgggc atcttgtagc tgagcacctt gttttccggc agatcgattt cgtcggtgcg
    15301 cacgcgcttg atggagtact caggcgtctg gatgtgcggg ttcggcacga tcatcagatc
    15361 gaccgagctc ttgtcctcga gcgtacgcat ccattcgcgc ttctcgttca tgaggtaggt
    15421 ggccacctcc accggtacct gcgcgacgat ctgggcggtc ttgtctttgc gggcctcttc
    15481 accgatgagg cgtaggatgg cgagcgtcat ggactcgacc gaacggatgc tgccgatgcc
    15541 ggtgcaccgc gggcacacca ggtggctgga atcgcccagc gagggacgca gccgctggcg
    15601 ggacatctcg agcaggccga agcgcgaaag gcgtccgatc tggatgcgcg cgcgatccat
    15661 cttcacggcg tcgcgcaggc gatcttcgac atcgcgctga ttcttcggcg actccatgtc
    15721 gatgaagtcg atgacgatca ggccgccgat gtcgcggatt cgcagctggc gcgcgatttc
    15781 gtccgcggct tcgaggttgg tgttggtcgc agtggtctcg atgtccgagc cgcgcgtggc
    15841 gcgcgcggag ttgatgtcga tggagaccag cgcctcggtg tagtcgacga cgatggatcc
    15901 gcccgaaggc agctccacct tgtgggcgta cgccgattcg atctggtttt cgatctggaa
    15961 gcgcgtgaac agcggaacgt cgtccgtgta gcgcttgagg cgcttctgcg cttcaggcgg
    16021 catgaaccgc gtcatgtatt cctgcgcggt cgcgaacgtg gcgtcgtcgt ccaccagcac
    16081 ttcgccgatg tcgtcggcga ggtagtcgcg caagctgcgc gtcaccgcat cgctttcgcg
    16141 atacaccagg tacggggcag ggcggtcttt ggtagccgcg tcgatctggt cccactggct
    16201 cttgaggttg tcgagatccc actgcagctc ggcggctgcg cggcccacgc cggcggtgcg
    16261 cacgatggcg cccatgccgt cggggatgac gagctcatcc atgacctggc gcatctggtc
    16321 gcggtcttcg ccttcgatgc ggcgcgacac accacccgca cgcgggttgt ttggcatgag
    16381 caccaggaag cggccggcca gcgagatgaa cgtggtgagg gcggcg
//
LOCUS       DASQJS010000038         5452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2223961, whole genome shotgun sequence.
ACCESSION   DASQJS010000038 DASQJS010000000
VERSION     DASQJS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2223961"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VEW08_01580"
     CDS             complement(<1..587)
                     /locus_tag="VEW08_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39446.1"
                     /translation="MYWAMFPIEFAAGKARNSTSPAHVPVLRLPAFWVMPILGEIQLS
                     AAASTVPAPSVLLIAGERLARYGFGDGHPFGPDRHAAFLAELVARGLDKRVQIMETRA
                     ATREELQSFHTPQYLDLVVERSRTGDGYLDGGDTPAWRGVYEAAADVVGGTLLAAEQI
                     MLGQARRAFIPIAGLHHAARGGAAGFCVFNDCGVA"
     gene            595..2247
                     /gene="ettA"
                     /locus_tag="VEW08_01585"
     CDS             595..2247
                     /gene="ettA"
                     /locus_tag="VEW08_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HYJ39447.1"
                     /translation="MNRVGKVVPPKRVILRDISLSFFPGAKIGVLGLNGSGKSTLLKI
                     MGGIDTNFEGEARAQSGIKVGYLPQEPQLDPDKDVRSVVMEGLGGAFAQVARFNEISD
                     KFAEPMDDEQMNKLLEEQSKLQDAIDAVGGWELERKLEIAADALRLPPWDAIINKLSG
                     GEKRRVALCRLLLSAPDMLLLDEPTNHLDAESIAWLEKYLEAYPSTVIAVTHDRYFLD
                     NVAEWILELDRGHGIPYKGNYSTWLEQKGARLAAEERTNEGLRKQLATELEWVRTSPK
                     GRRAKNKARVARYEELSSREFQERNETNEIYIPPGERLGDVVIEAKNLRKSYGDRLLI
                     DDLNFNLPAGGIVGIIGPNGAGKTTLFRMMSGLEKPDSGELRIGPTVKLAYVDQSRQS
                     LDDKKTVWEEISGGLDMIKVGNYETPSRGYVGRFNFKGTGQQQIIGELSGGERNRVHL
                     AKVLKSGGNVLMLDEPTNDLDVETLRALEEALLAYPGCAVVISHDRWFLDRIATHILA
                     FESNSEVVWFEGNFQDYMEDLRRRKGEEATQPHRIKYKPLTR"
     gene            2283..2984
                     /locus_tag="VEW08_01590"
     CDS             2283..2984
                     /locus_tag="VEW08_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39448.1"
                     /translation="MNVLIDSTGAMYFGDERATTRNCPHCQALSRMSPTALPTFDELL
                     LQKPRSIGIVMRCHACNAPVFLRYAVRAYASNRVELSPQAFDVERPIEKFAFNYVPEG
                     VEIFFREALICYSHNQFQAFASMCRRSAGAAFVDLGNSTKLRLFDSLSDVREMAEIDE
                     ETFAVVMRVIFAGHADPQLDLPFINTYQAAVLLEVMKDFLYQAYVRKGRLQHAMKVRR
                     FFVDEHSARRARSED"
     gene            complement(2981..3220)
                     /locus_tag="VEW08_01595"
     CDS             complement(2981..3220)
                     /locus_tag="VEW08_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYJ39449.1"
                     /translation="MPLVVNLDVMLARRKMRLNELAELVGITAQNLSVLKTGRAKAIR
                     FSTLEKLCEVLDCQPGDLFAFENPAAEEEASAAAV"
     gene            complement(3234..3854)
                     /locus_tag="VEW08_01600"
     CDS             complement(3234..3854)
                     /locus_tag="VEW08_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39450.1"
                     /translation="MNASASALPREDEVQSKIRKVRQFGRIARVVCAAIFGFGLVGIV
                     FMLVISVPTKIPGPIKGNGGAWDILNSLLNPYQVKVFAGLGVSALAGVWLAAVYQLYR
                     LFGDLAVGAIYTPENVRRVRHVGLLLLLLTVLMDIVIPAAFVVAHGFIEPSIPLDLDL
                     LFPPPPELFEDFISPGLILLVSWIMDVGLREKEHADALRRDADLVI"
     gene            complement(3988..>5452)
                     /locus_tag="VEW08_01605"
     CDS             complement(3988..>5452)
                     /locus_tag="VEW08_01605"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HYJ39451.1"
                     /translation="TWTPQLRTDLGATVLLLVDLGKGANRLGASALAQVFGELGSEPA
                     DLASPVLLTQLAAVLTDLRAQNLVLAYHDRSDGGVFATLVEMAFAGHCGLDIKLPSSA
                     DGGGATGAAGALFSEELGVVLQVKAEHAAAVHSVFVRHGLGEWTHAIGAPTRDMRVVV
                     QSGASRIDESWEDLRRAWSETSWRMRERRDEPSCAHEEFAAACDTGAPGLNVALTFDP
                     NDDVSVPFIATSRPKVAVLREQGVNSQIEMAAVFDRVGFEPHDVHMSDVLSGRVKLDS
                     FRGLVACGGFSYGDVLGAGEGWAKSILYHAGAREQFQRFLDRSDTFTLGVCNGCQMFA
                     ALKEIIPGAQSWPRFVRNRGEQFEGRFSLVELQPSPSIFLAGMDGSMLPIAVAHGEGR
                     AEFASDDAARAFTASSLVSARYIEGNRKVATNYPANPNGSPFGIAAITNEDGRVTLTM
                     PHPERSFRYAQNSWRPDGAGEYSGWYRMFGNARRWVG"
BASE COUNT         1096 a         1727 c         1710 g          919 t
ORIGIN      
        1 atagccacac cgcagtcatt gaaaacgcag aagcccgcag ctccgccgcg cgccgcgtga
       61 tgcaggccgg cgatgggaat gaacgcgcgc cgcgcctgac caagcatgat ctgctcggcg
      121 gcgagcaacg ttcctcccac aacatccgcg gcggcttcat agacaccgcg ccaggccggc
      181 gtgtcgccgc catcgaggta accgtcaccg gtgcgcgagc gttcgacgac caggtcgagg
      241 tactgcggcg tatgaaagga ttgcaattct tcgcgcgtgg ccgcgcgggt ttccatgatc
      301 tgcacgcgct tgtccagccc tcgtgcgacc agctcggcga gaaaggcggc gtgacggtct
      361 ggaccgaaag ggtggccgtc gccgaagcca tagcgcgcca ggcgttcgcc ggcaatcaat
      421 aaaacagacg gagccggcac ggttgacgct gcagcggaca gttgtatctc cccaaggatc
      481 ggcatcaccc aaaacgcggg caaacgtagc acaggaacgt gggcggggga tgtagaattc
      541 cgcgcctttc ccgccgcaaa ctcaatcgga aacatggccc aatacatcta cacgatgaac
      601 cgggttggca aggttgtgcc acccaagcgc gtcatcctgc gcgacatcag tctttcgttt
      661 tttcccggcg ccaagatcgg cgtactcggc ctcaacggct cgggcaaatc cacgctgctc
      721 aagatcatgg gtggcatcga caccaacttc gagggtgaag cccgcgcgca atccggtatc
      781 aaggtcggtt acctgccgca ggaaccgcag ctcgaccccg acaaggacgt gcgcagcgtg
      841 gtcatggaag gtctcggcgg agcgttcgca caggtcgcgc gcttcaatga gatcagcgac
      901 aagttcgccg aacccatgga cgacgagcag atgaacaagc ttctcgagga gcagtcgaag
      961 cttcaggacg ccatcgatgc ggtgggcggt tgggagctcg aacgcaaact cgagatcgcc
     1021 gcggacgcgc tgcgcctgcc accgtgggac gccatcatca acaagctctc gggtggcgag
     1081 aagcgtcgcg tggcgttgtg ccgcctgttg ctgtccgcgc ccgacatgtt gttgttggac
     1141 gagcccacta accatctcga cgccgaatcc atcgcgtggc tcgagaaata tctggaagcc
     1201 tatccgtcga cggtcatcgc ggtgacacac gatcgctact tcctcgacaa tgtggccgag
     1261 tggatcctcg agctcgaccg cggccatgga attccctaca agggcaacta ctccacgtgg
     1321 ctcgagcaaa agggcgcgcg actcgccgcg gaagagcgaa ccaacgaagg gctgcgcaag
     1381 cagctggcga cggaactcga atgggtgcgc acgagcccga aaggccgccg cgccaagaac
     1441 aaggcgcgcg tcgcgcgcta cgaggaactc tcctcgcgcg aattccagga acgcaacgag
     1501 accaatgaaa tctatattcc gccgggcgag cgcctaggcg atgtggtcat cgaagcgaag
     1561 aatcttcgca agtcgtacgg cgatcgcctg ctgatcgacg acctcaactt caatctgccc
     1621 gcgggtggca tcgtcggcat catcggaccg aacggcgccg gcaagaccac gctgttccgc
     1681 atgatgtcgg ggctcgaaaa accggactcc ggcgagttgc gcatcggacc gacggtcaag
     1741 ctcgcctacg tcgatcagtc gcgccagtcg ctcgacgaca aaaaaaccgt ctgggaagaa
     1801 atctccggtg gtctcgacat gatcaaggtc ggcaactacg aaacgccgtc gcgaggttat
     1861 gtcggacgct tcaacttcaa gggcacgggc cagcagcaga tcatcggtga gctctcgggc
     1921 ggtgaacgca atcgcgtgca cctggccaag gtgctcaagt ccggcggtaa cgttctcatg
     1981 ctcgacgaac ccaccaatga cctcgacgtc gagacgttgc gtgccctgga agaggccttg
     2041 ttagcctatc cgggctgcgc agttgtcata tcgcatgaca gatggttcct cgatcgcatc
     2101 gctacgcata tcctcgcgtt cgaaagcaac tccgaggtgg tatggttcga gggcaacttc
     2161 caggactaca tggaagacct caggcggcgc aagggcgaag aagccacgca gccacatcgg
     2221 atcaagtaca aaccactgac tcggtagcaa gcccggccaa acgggccgaa ggtaagcgga
     2281 cgatgaacgt gttgatcgac tcgacgggcg ccatgtattt cggcgacgag cgcgcgacga
     2341 ctcgcaattg cccacattgc caggcgctgt cgcgcatgag cccgacggcg ctgccgacgt
     2401 tcgacgaact gctgctgcaa aagccgcgct ccatcggcat cgtgatgcgc tgtcacgcct
     2461 gcaatgctcc cgtgttcctg cgttacgccg tgcgcgcgta cgccagcaat cgcgtcgagc
     2521 tgtcgccgca ggccttcgac gtcgagcggc ccatcgagaa gttcgcgttc aattacgttc
     2581 cggaaggcgt ggagattttt ttccgcgagg cactgatctg ctattcgcat aatcaattcc
     2641 aggcgttcgc atccatgtgc cggcgctccg ccggcgccgc attcgtcgat ctcggcaact
     2701 ccaccaagct gcggctgttc gattcgctgt ccgacgtgcg cgagatggcg gagatcgatg
     2761 aagagacctt cgcggtggtc atgcgtgtca tcttcgccgg tcacgccgat ccgcagctcg
     2821 acctgccttt catcaacacc taccaggcag cggtgttgtt agaggtgatg aaagacttcc
     2881 tgtatcaggc ctacgtgcgc aagggccgcc tgcagcacgc aatgaaggtg cgcaggtttt
     2941 tcgtcgatga gcactcggca aggcgcgcgc ggagcgaaga ctagaccgcc gccgctgacg
     3001 cttcctcttc ggccgccgga ttctcgaacg cgaacagatc gcccggctgg caatcgagca
     3061 cctcgcagag tttctcgagt gtgctgaagc ggatggcctt cgccctgccc gtcttcaaca
     3121 ccgaaaggtt ctgcgcggtg atgccgacga gctcggcgag ctcgttcagg cgcattttgc
     3181 gccgcgcgag catcacatcc aggttcacga cgagtggcac gtggatgctc cgttcagatc
     3241 acgagatcgg cgtcgcgccg tagcgcgtcc gcatgttctt tctcgcgcag gccgacgtcc
     3301 atgatccacg agacgagcag gatgagcccg ggcgaaatga agtcttcaaa caactccggc
     3361 ggcggcggga acaggagatc gagatcgagc gggatcgacg gctcgataaa accgtgcgcg
     3421 acgacgaacg cggctggaat cacgatgtcc atgagcactg tcagcaggag caacagaagg
     3481 ccaacgtgcc ggacgcggcg tacgttctcg ggcgtgtaga tcgcgccgac ggccagatcg
     3541 ccgaacagac gatagagctg gtacacggcg gcgagccaga cgccggccaa cgcgctcacg
     3601 ccgagacccg caaacacctt cacttgatac gggttgagca gggaattcaa gatgtcccag
     3661 gcaccaccgt tgcccttgat cggtccgggg atcttggtgg gcacggagat gaccagcatg
     3721 aacacgatcc cgaccaggcc gaatccgaag atcgccgcgc acacgacgcg ggcgatgcgg
     3781 ccgaactgcc gtaccttgcg aatcttcgat tgaacctcgt cctcgcgggg gagtgctgaa
     3841 gctgaagcgt tcaccggggt tactccgttt gtggtgcgcg tgtcgcggat cggtggcgga
     3901 tcctagcctc agcaaatctc gctttgcaag cattttttat cgtttatcga tgtgcatcga
     3961 ccgtttaacg atacctgccg gcgaaactta gcccacccag cggcgcgcat tgccgaacat
     4021 gcggtaccag ccgctgtact cacccgctcc atccggccgc cacgaattct gggcatagcg
     4081 gaacgagcgc tcggggtgcg gcatggtcag cgtcacgcga ccatcttcgt tggtgatggc
     4141 ggcgatgccg aacggcgaac cgttggggtt cgccgggtag ttagtggcca ccttgcggtt
     4201 gccttcgatg taacgcgcgg ataccaggct gctggccgtg aaggccctgg ccgcgtcgtc
     4261 gctcgcgaac tccgcccggc cctcaccgtg cgccacggca atcggcagca ttgatccgtc
     4321 catgccggcg aggaagatcg atggcgacgg ctgcagctcc accagcgaga agcgcccttc
     4381 gaattgctcg ccgcggttgc gcacgaagcg cggccagctc tgcgcgcccg ggatgatctc
     4441 cttgagcgcg gcgaacatct ggcagccatt gcagacgcct agcgtgaatg tgtcggaacg
     4501 atcgaggaag cgctggaact gctcccgcgc gccggcgtga tagaggatgg acttggccca
     4561 gccctcgccg gcacctaaca cgtcgccgta ggaaaacccg ccgcaggcca cgagcccgcg
     4621 gaaggaatcg agcttgacgc gccccgagag cacgtcgctc atgtgcacgt cgtgcggctc
     4681 gaagccaacg cggtcaaata cggccgccat ttcgatctgg ctgttgacgc cctgctcgcg
     4741 cagcacggca accttcgggc gcgaggtggc gatgaacggc acggacacgt cgtcgttcgg
     4801 atcgaacgtg agtgcaacgt tgagaccggg cgcaccggtg tcgcaggcgg cggcgaattc
     4861 ctcgtgcgcg caggaaggct cgtcgcgacg ctcgcgcatg cgccaggagg tttcagacca
     4921 ggcgcgccgc aggtcttccc acgattcatc gatgcgggag gcgcccgact gcacgaccac
     4981 gcgcatgtcg cgcgtgggtg caccgatcgc gtgcgtccac tcgcccaggc cgtgccgcac
     5041 gaaaaccgag tgcaccgcgg cagcatgctc ggccttcacc tgcaacacca cgccaagttc
     5101 ttcggagaac agcgcgccgg cagcgcccgt ggcgccgccc ccatccgcgg acgagggtag
     5161 tttgatgtcg agtccgcagt ggccggcaaa cgccatttcc acgagcgtcg caaatacgcc
     5221 gccgtcggag cggtcgtgat aggccagcac caggttctgg gcgcgaaggt ccgtcaacac
     5281 ggcggccagt tgcgtgagca gcaccggcga agcgaggtcc gcgggttcgc tgccgagttc
     5341 gccgaacacc tgcgcgagcg ccgaagcgcc aagacggttc gcgcccttgc cgaggtcgac
     5401 gagcagcaac accgtcgcac cgagatcggt gcgcagctgc ggcgtccagg tc
//
LOCUS       DASQJS010000039         3616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_652807, whole genome shotgun sequence.
ACCESSION   DASQJS010000039 DASQJS010000000
VERSION     DASQJS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652807"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VEW08_01610"
     CDS             complement(<1..1055)
                     /locus_tag="VEW08_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HYJ39452.1"
                     /translation="MRWIGSRLAMWFLAWGFAGVAAAAAAAPSPVIQYAEPVVLALKS
                     SSAQFEAYGRRFSLSLIDNGRVLDKLSAQRKAQLQSYKLLRGSLDGAPGSWVRLTESP
                     AGVEGAIWDGHDLYTVTRYDRIAPFLTTPLDAAPGQTVVYRLSDSLDVLPRDFCALSD
                     AEKTSKATNGLDQYVAVMQEIEAGVYTPQITRQIEVSLIGDTAFQAAESNDPTAAMLA
                     RLNIVEGIFSEQVGLLILATDVRLMQVGADPFTATKGSTLLEQLGAYRQVTPEVRARG
                     LAHLMTGKDLDGTTAGIAYVRTVCDVERGVSVSSRSYGTTISALIMAHELGHNFGAEH
                     DGEAGTACANTSGGFIMA"
     gene            1277..1477
                     /locus_tag="VEW08_01615"
     CDS             1277..1477
                     /locus_tag="VEW08_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39453.1"
                     /translation="MRADSLAMLVSADGARTIYSTSGYVVLVSIAGSPASAALMNVDA
                     PLILMSLGTSSNAFRGLAVVAR"
     gene            complement(1491..1916)
                     /locus_tag="VEW08_01620"
     CDS             complement(1491..1916)
                     /locus_tag="VEW08_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HYJ39454.1"
                     /translation="MSELSLVYPMLALVLLTFGVLVALFRSRVRMVREGLAPVSYFRT
                     FQGSVVEPEFTVKPQRHFANLFEAPTLFYVGCLAAMVTGVTGTLVVVLAWAYVALRYL
                     HAYIHLGGNRVRYRMRAYFASWVCLLAMWITVVVGVALR"
     gene            1972..2502
                     /locus_tag="VEW08_01625"
     CDS             1972..2502
                     /locus_tag="VEW08_01625"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HYJ39455.1"
                     /translation="MNRVLKTGLAALALATVAGSLMAHDDDDRGRDGRFVVRTNLEGF
                     QEVPAVSTVASGRFRAVIDTKANTITWKLNYEGLEGAVQQAHVHFGQLSVNGGVSFFL
                     CTNLGNGPAGTLTCPEGPAELTGVITPELVVGPAGTGIEAGAMAEIIAAIKNGTAYAN
                     VHSSKWTLGEIRGQLR"
     gene            2731..3039
                     /locus_tag="VEW08_01630"
     CDS             2731..3039
                     /locus_tag="VEW08_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HYJ39456.1"
                     /translation="MTKLYVGNLPFSATEDQVRALFATHGTVDKVSLVNDRETGRPRG
                     FGFVEMPNADATRAMQALDGKDFGGRPLKVNEAQERERGAPRGNFGGGGGGGGGRGRF
                     "
     gene            complement(3162..>3616)
                     /locus_tag="VEW08_01635"
     CDS             complement(3162..>3616)
                     /locus_tag="VEW08_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYJ39457.1"
                     /translation="TDQHGSAMRISDLAGAPVLVFFGFTHCPDICPTMIQQLALIKSS
                     RAKELAGLRIAFITVDGERDTPAVMKEYLKRFPAEFVGLTAPAEQVRNLALQFSAPFF
                     KDPPKDGEYAVQHSSRLFALDKQGRLRAEIYDASPEAVVGIAQALLAE"
BASE COUNT          683 a         1131 c         1176 g          626 t
ORIGIN      
        1 gccatgatga aaccgccgct ggtgttcgcg caggccgtgc ctgcctcgcc gtcgtgctcg
       61 gcgccgaaat tgtggccgag ttcgtgcgcc atgatgagcg cggagatcgt ggtgccgtag
      121 gagcgcgagc tgaccgagac accacgttcg acgtcgcaca ccgtgcgaac gtaggcgatg
      181 cccgccgtgg tgccgtcgag atccttgccc gtcatcaggt gcgcgaggcc gcgcgcgcgt
      241 acttccggcg tgacctggcg atacgcgccc agctgctcga gcagcgtgga gcccttggtc
      301 gcggtgaagg gatccgcgcc cacctgcatc agccgcacgt cggtcgccag gatcagcagg
      361 cccacctgct cgctgaaaat gccttcgacg atgttgaggc gcgccagcat ggcggcggtg
      421 gggtcattgg attccgcggc ctggaacgcc gtatcaccga tcagcgagac ctcgatttgc
      481 cgcgtgatct gcggggtgta cacgcccgct tcgatctctt gcatcacggc cacgtactgg
      541 tcgaggccgt tcgtggcctt tgaagttttt tcagcgtcgg aaagtgcgca gaaatcgcgc
      601 ggcagtacat cgagggaatc cgagagccgg tacaccaccg tctggcctgg cgcggcgtcc
      661 agcggcgtcg tcaggaaagg cgcgattcgg tcgtagcgcg tgacggtgta gaggtcgtga
      721 ccatcccaga tcgcgccttc cacgcccgcg ggggattcgg tgagccgtac ccaggatccg
      781 ggcgcgccgt cgagcgagcc gcgcagcagc ttgtaggact ggagttgcgc cttccgctgt
      841 gcggaaagct tgtcgagcac gcggccgttg tcgatgagcg aaagcgaaaa gcggcggccg
      901 taggcctcga attgcgcgct gctggatttc agtgccagga ccacgggctc ggcgtactgg
      961 atgaccggcg atggcgcggc agcggcggcc gcggctacgc ccgcgaaacc ccacgccaga
     1021 aaccacatcg caagccggct accgatccat cgcacccaga cgtcccctgc tagtcgttgt
     1081 tcttctccga tgagctgtaa ggctacagaa ctgccctcat cggagctggg actctagacg
     1141 ctgagttggc gtaggcgggg acgcgtacct gggtgacttc gcgattaagt gacgcgggcc
     1201 tcaggaacat aatgtgcggg cataacgtct ctcccgaatc gcgactccga tggccgcagt
     1261 ggagggatac aacggaatgc gtgccgattc actggcgatg ctggtcagcg cggacggcgc
     1321 gcgaaccatc tattcaacca gcggctacgt tgttctggtc agcattgccg gcagtcccgc
     1381 aagtgcagcg ctgatgaatg tcgatgcgcc gctgatactc atgagcctcg gtacgagcag
     1441 caacgcgttc cgcggtcttg ccgtcgtcgc gagatgagca cgctctgacg tcaacgcagg
     1501 gctacaccca ccaccacggt gatccacatc gccagcaggc acacccagct cgcgaagtac
     1561 gcgcgcatgc gataccggac ccggttcccg ccgaggtgga tataggcatg cagatatcgc
     1621 aacgcgacat acgcccaggc caggaccacg accagcgtgc cagtgacacc ggtaaccatg
     1681 gccgccaggc agcccacata gaagagagtt ggagcctcga acaggttggc gaagtgcctc
     1741 tggggcttca ccgtaaattc cggctccact acggaaccct ggaacgtcct gaagtaactc
     1801 accggggcta ggccctcgcg aaccatacgg acccgggatc ggaacaacgc gacgaggacg
     1861 ccaaaggtga gcagcaccag cgccaacatc gggtaaacga gactgagttc agacatgggg
     1921 actcctgcgg tacgcgaggg atgcatgtaa ccaaacagac aggggaaagt catgaatcga
     1981 gtattaaaga cgggactggc cgcgctcgcg ctggcgacgg tggccggtag tttgatggcg
     2041 cacgacgacg acgaccgggg cagggatggc aggttcgtgg tgcgcacgaa cctggagggc
     2101 tttcaggaag taccggcggt ctcgaccgtc gcctcgggtc gcttccgcgc ggtcatcgac
     2161 acgaaggcca atacgatcac ctggaagctc aactacgagg gcctggaggg cgccgttcag
     2221 caggcccatg tgcacttcgg ccagctgagc gtgaatggcg gcgtcagctt cttcctctgc
     2281 actaacctgg ggaatggccc cgcggggacg ctgacctgcc ctgaaggccc ggccgagctc
     2341 acgggggtga tcaccccgga actggtggtc ggtcctgctg gtaccggtat cgaagccggc
     2401 gccatggcgg aaatcatcgc ggctatcaag aacggcacgg cctacgcgaa cgtccactcg
     2461 tcgaagtgga ccctcggcga aatccgcggc cagctgcgtt aaacaaggcg atttggccac
     2521 cggcccgtgc cccggaaggg ggcgggccgg ggccttccct acatttttga ccagacgatg
     2581 tggcctccgc ctaaggtttc atgtaccgtc gccggcggct tgcaattctt cgtcgatacg
     2641 gcgcctctgc gcgccgcaac cccaaagcat tagcgaacca gcagacaggg cttggcctgt
     2701 catctgttca atattcttgt gagtgattct gtgacgaaac tttacgttgg taatcttcct
     2761 ttctccgcca ccgaagatca ggtgcgcgcc ctcttcgcaa cccacggcac cgtcgacaag
     2821 gtgtcgctcg tgaacgatcg cgagaccggc cgcccgcgcg gcttcggttt cgtcgaaatg
     2881 ccgaatgccg atgccacccg cgccatgcag gcgctcgatg gcaaagactt cggtggtcgt
     2941 ccgctgaagg tcaatgaagc ccaggagcgc gagcgcggcg ccccgcgcgg caacttcggt
     3001 ggtggcggtg gtggcggcgg tggccgcggc cgcttctaaa ccacgaagca attgagattg
     3061 agcggcgccc tggtggcgcc gctttttttt gggtgtcgga aggtgccggg tggggaaagt
     3121 ggggagtgcg gcactcccca ctttccccac ccggcaccga cctactccgc aaggagcgcc
     3181 tgcgcgatcc caactaccgc ctccggcgag gcatcgtaga tctcggcgcg cagcctgccc
     3241 tgcttgtcga gagcgaagag ccggctcgag tgctgcacgg cgtattcgcc atccttgggc
     3301 ggatccttga agaaaggcgc cgagaattgc agcgcgagat tgcgcacctg ttccgccggc
     3361 gcagtgagac ccacgaattc cgcggggaag cgcttcaggt actccttcat cacggccggt
     3421 gtatcgcgct cgccatccac cgtgatgaag gcgatgcgta accccgcgag ctccttggcg
     3481 cgcgacgact tgatgagcgc gagctgctga atcatggtcg ggcagatatc cgggcaatgg
     3541 gtgaacccga aaaacaccag caccggcgca ccggccaggt cgctgatgcg catcgccgat
     3601 ccgtgctgat ccgtca
//
LOCUS       DASQJS010000040         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2536232, whole genome shotgun sequence.
ACCESSION   DASQJS010000040 DASQJS010000000
VERSION     DASQJS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2536232"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1513
                     /gene="gltD"
                     /locus_tag="VEW08_01640"
     CDS             29..1513
                     /gene="gltD"
                     /locus_tag="VEW08_01640"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009342 - glutamate synthase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0045181 - glutamate synthase activity,
                     NAD(P)H as acceptor [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase small subunit"
                     /protein_id="HYJ39458.1"
                     /translation="MGKPTGFIEYLRELPVDRTPIERVRDWNEFHHHMDEKRLRQQGA
                     RCMDCGVPFCHTGKLISGMASGCPVNNLIPEWNDLVFRGLWREALDRLHRTNNFPEFT
                     GRVCPAPCEGSCVLGINAPPVTIKNIENAIVDKGFDEGWIVAEPPTTRTGKKVAVIGS
                     GPAGLAAAAQLNKAGHSVTVLERADRPGGLLTYGIPNMKLDKREVVARRISLLEKEGI
                     KFVCNATVGENVEAELLVKDFDATVICTGATLPRDLPVEGRNLKGVHFAMDFLTVSTQ
                     AVLRDAPDATPISAKGLDVVVLGGGDTGTDCVGTSMRHGCKSLTQLEIMAMPPMDRAA
                     DNPWPEWPKVYKMDYGQEEAAAKFGADPRVYLTTVKKFKDDGAGNLAAVVTVNIKWEK
                     NDKGQFIPVEQPGSEKEHPAQLVLLAMGFLGPEQSLLKDLKVETDARSNVKAEYGKYG
                     TSVPGVFAAGDARRGQSLVVWAINEGRGAARECDRWLMGATELP"
     gene            complement(1562..>2905)
                     /locus_tag="VEW08_01645"
     CDS             complement(1562..>2905)
                     /locus_tag="VEW08_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HYJ39459.1"
                     /translation="EASPARPPSVAQEVLALLRLQTWGLVDGARLFGRDVTLILLGVF
                     SLVLWIGLDRLRYGGEVELFIYGVTENAAMVLAVLAVAWLMSRASRPRIELRQGLLLV
                     LGFLPIFIVMFWIAEWLPQLAIFAIAILVTGWAERFFGAGLRSLTGKRQWMAVTSSLI
                     GVVTLVYLSSQVYYTPGFWIEREPDAEKAAQTRRKNEQIVFEQSARINADIASLAPRE
                     AGQPNVFFLGFAGYGGQKVFAEEIGLAAKRVGERYGSAQRSVLLVNDHRDALKYPLAS
                     APSLRHALNALSQQMNLDEDVLFLALSSHGSEGGTVSVSSELGYWRDLGATDLADMLR
                     ESGIRWRVIVVSACYAGTFVEPLRDDNTIILTAAAADRTSFGCSDDNDLTYFGEAFYR
                     DALPKAANLRAAFDTARAAILERESGEGMTPSNPQAHFGAAIEQKLAAMEATRTN"
BASE COUNT          589 a          933 c          922 g          461 t
ORIGIN      
        1 aaaaaagtac cagaacagaa gtagatagat gggaaagcca acaggattta tcgagtactt
       61 gcgcgagctg ccggtggatc gcacgccgat cgaacgcgtg cgcgactgga acgagttcca
      121 ccaccacatg gacgagaagc gcctgcgcca gcagggcgcg cgttgcatgg actgcggtgt
      181 gccgttctgt cacacgggca agctgatctc cggcatggcc tcgggctgcc cggtgaacaa
      241 tctcatcccg gagtggaacg acctggtgtt tcgcggcctg tggcgcgagg cgctcgaccg
      301 cctgcatcgc accaacaact ttccggagtt caccggccgc gtgtgcccgg cgccgtgtga
      361 aggctcgtgc gtgttgggca tcaacgcgcc gccggtgacc atcaagaaca tcgagaacgc
      421 catcgtcgac aagggcttcg acgaaggctg gatcgtcgcc gaaccaccga cgacgcgaac
      481 cggcaagaag gttgccgtca tcggctcggg tcccgcggga ctcgccgccg ccgcgcagct
      541 caacaaggcc ggtcactccg tgaccgtgct cgagcgcgct gatcgtcccg gtggccttct
      601 gacctacggc attccgaaca tgaaactcga caagcgcgaa gtggtcgcgc gccgcatatc
      661 gctgctcgag aaggagggca tcaagttcgt ttgcaatgcg acggtgggcg aaaacgtcga
      721 agccgaacta ctggtcaagg atttcgacgc gaccgtgatc tgcaccggcg ccactctgcc
      781 gcgcgacctg ccggtcgaag gccgcaatct gaagggcgtg cacttcgcca tggacttcct
      841 caccgtgagc acgcaggccg tgctcaggga tgcgccggac gcgacgccca tctcggcgaa
      901 gggcctggac gtggtcgtgt taggcggtgg cgacaccggc accgactgcg tcggcacctc
      961 gatgcgccat ggctgcaaga gcctcacgca gctcgagatc atggcgatgc cgcccatgga
     1021 tcgcgccgcg gacaatccgt ggcccgaatg gcccaaggtc tacaagatgg actatggcca
     1081 ggaagaggct gcggcgaagt tcggagccga tccacgcgtc tacctgacca ccgtcaagaa
     1141 gttcaaggat gacggcgccg gcaatctcgc cgcagtcgtc accgtcaaca tcaaatggga
     1201 aaagaacgac aagggtcagt tcattcccgt cgagcagccc ggcagcgaga aagagcatcc
     1261 ggcgcagctc gtgttgttag ccatgggttt cctcggccca gagcagtcgc tgctcaagga
     1321 cctgaaggtg gaaaccgatg cgcgcagcaa tgtgaaagcg gagtacggca agtacggcac
     1381 cagcgtgccg ggtgtgttcg cggcgggcga tgcgcgccgt ggccagagcc tggtcgtgtg
     1441 ggccatcaat gaaggccgtg gcgccgcgcg cgagtgcgac cgctggctca tgggcgcgac
     1501 cgaactcccg taaatgggga cctggatggg tggggacagg cttgagtctt gcccccacac
     1561 gttaattcgt tcgagtggct tccatggccg cgagcttctg ctcgatcgcc gcgccgaagt
     1621 gcgcctgcgg gttcgagggc gtcattcctt ctccgctctc acgctcgagg atcgcggcgc
     1681 gcgcggtgtc gaacgcggcg cgcaggttgg ccgccttcgg cagcgcatcg cgataaaacg
     1741 cttcgccaaa gtacgtgagg tcattgtcat cggagcagcc gaatgacgtc cggtccgcgg
     1801 ccgccgcggt caggatgatc gtgttgtcgt cgcgcagcgg ctcgacgaag gttcccgcat
     1861 aacacgcgga cacgacgatc acccgccagc gaatgcccga ctcgcgcaac atgtcggcga
     1921 ggtcggtcgc gccaagatcg cgccaatacc caagctcact gctgactgag accgtgccgc
     1981 cctcggagcc gtgcgatgac agcgcgagaa agagcacgtc ttcgtccagg ttcatttgct
     2041 ggctcagcgc gttcagcgcg tgacgcagcg acggcgcgct ggccaacgga tatttcagag
     2101 catcgcgatg atcgttgacg agcagcaccg atcgttgcgc gctcccgtag cgctcgccga
     2161 cgcgcttcgc ggccaggccg atctcctcgg cgaaaacctt ctggccgccg tagcccgcga
     2221 aacccagaaa gaaaacattc ggctgccccg cttcgcgcgg cgccaaagac gcaatgtcgg
     2281 cgttgatcct cgccgactgc tcgaacacga tctgttcatt cttgcgccga gtctgggccg
     2341 ccttttcggc gtccggctct cgctcgatcc agaaacccgg cgtgtaatac acctggctcg
     2401 atagatagac cagggttacc acgccaatga gcgacgaagt gaccgccatc cactgccgct
     2461 tgccggtcag cgaccggagt cccgcgccga agaatctctc agcccagcca gtcactaaga
     2521 tcgcaatggc gaagatggcc aactgcggca accactcggc gatccagaac atgacgatga
     2581 aaatcggcag aaagcccaac acgagcagca ggccctggcg caactcgatg cgcggtcggc
     2641 tggcgcgcga catcaaccag gcaacggcca gcaccgcgag caccattgct gcgttctcgg
     2701 tgaccccata gatgaacagc tcgacctcgc ccccgtagcg gagccggtcg agaccgatcc
     2761 acagcaccag tgaaaaaacg ccgagcagaa tcagcgtgac atcgcgaccg aacagccggg
     2821 cgccgtcgac gagcccccag gtttgcaggc gaagcagcgc aagcacctct tgcgccacgg
     2881 aaggtggccg ggcgggggag gcctc
//
LOCUS       DASQJS010000041         7070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4106502, whole genome shotgun sequence.
ACCESSION   DASQJS010000041 DASQJS010000000
VERSION     DASQJS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4106502"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VEW08_01650"
     CDS             complement(<1..418)
                     /locus_tag="VEW08_01650"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39460.1"
                     /translation="MEAIVALLSDPQVWIAFFTLTALELVLGIDNIIFISILVDKLER
                     VQREKARRIGLFLAMFMRIGLLMVLSWIVGLTAPLFTVTGTDISGRDLILIAGGVFLV
                     WKSTMEIHQLTEGEEGHASKSVKASFGAIILQIIIID"
     gene            469..2385
                     /locus_tag="VEW08_01655"
     CDS             469..2385
                     /locus_tag="VEW08_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HYJ39461.1"
                     /translation="MIKVIKNLIASFLFLALSAQAGARLASGEVHVWQVQEITFAAAR
                     DYSNPYADVELWVELEGPDFFRRRIYGFWDGGRTYKVRLVATHPGEWTWRAGSSRDDP
                     GLAGAGKFRAVAWSEAELAENPNRRGFVRNSENGHALRYADGSPFFLTGDTWLAASTW
                     RLPFKGTRAAADYVPAEGISFEEAVAWRKRQGYNSISFISAFPNWAADEHGATFANKD
                     GVYLRNAWEKFGHWAPNAKISTADGATTTAKDMHDEAGNRPFEVFADREGLANFDALN
                     PKYFASLDRKMQHLSDQGFVPFLETIRRDNAPSWKRYFDFNVSYARFVQYLVARYGAY
                     NLVFSGIHLDWIPKDFSLTADEFNDALTWHYRKYGPLPFGQPYTALIDRSTLKAFGTG
                     ARAPWLTMHTVGNNPRNHAIYESIEELFGLTPGMPAANLEPYYTGWNHSINRPGGEIP
                     DENSARDNYFARAMMYGSVLSGALAGHVHGTAAYDVTSTGEPAGWRPHIWTALRYESG
                     AQMQHLRAFVLSQGSLYQRLEVASRDLDPRSIPGAAADGLDGWSFLMRTPAKDFALAY
                     FENKAMRARMKGFTPAAKYQWTWFDPRTGQWGRAVSVKADASGVISTPPFAAGGNQAT
                     NDVAAKIVRVVRRP"
     gene            complement(2372..5350)
                     /locus_tag="VEW08_01660"
     CDS             complement(2372..5350)
                     /locus_tag="VEW08_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ39462.1"
                     /translation="MSHSHTSNPGTPAEHITPYDRLALDEATLVALLASGTPNEGLVE
                     YFGAELHAELASLARATTRKRKTRATPRRRVYVLPGIMGSQLGFIRGGKRPNDILWLD
                     PIDIAFGRLTELRLNDASRIVALGAMNYSYLKFTLSLRKAGFDAVLLDYDWRRDIATL
                     GTELAARIAADGRDEVALIGHSMGGLVARAALTHAAGSRVSQLIMLGTPNSGSLAAVQ
                     ALRGTYSVVRKIAMLDLRHDAEYLARNVFASFPGLHELLPANKSVSDLDLFDAAVWPA
                     KGPGPDAALLRSASGLEQRMAPADARFNMVVGCNRTTATGVALRDGDFEYEYSLQGDG
                     TVPIELARLAGARHSYVECGHSDMTLSDRVIDGTIDLLETGATQRFAAAPPVKRGSLT
                     RVRDTELRAQYQGKIDWPHMTPEQRRLFLDTLNEAPRGRVHQRPQRAGKIQPLSINVR
                     VGDVAKARAAATAVAVLRGVPASGAAADIDQRLGGIIGDWLQHRVVSGDAGHVTPIPR
                     SLVKKTRGPRTSFLLVGLGRFDRLSLDVIEHAAENLARFAEAPQYRSIATVAWGAAAG
                     IEPADSFAAQLRGLLRARAAGEAGISRVDLHVLTRADAQTVHARLRDFVKSRPAGMLR
                     LRPLAPSQKSAATGVRRVPGTAHVIVAAESRRGKRETWRASLLTGGSSAAIFSQSQEF
                     AAQALDRLVREFQGEALTPARVKSLGGKLGALTLHPSLASALYAARGQALSVVHDAAS
                     SRIPWEALNIRGWVPALDGGLSRRYATADLVPARFDTGRREQRELGVLLIANPTGDLP
                     GAESERERIAHLLGRGKTVRLTEVAGNAATLARVTAEFESGRYDVIHYAGHASFDEVL
                     PGDGGLALADGVLTGAHLSALGRLPPLVVFNACESARLRRNATPKRSATQRGQGIARN
                     LSLAETLLRAGLAHYVGTHWPVEDASATAFATVFYRELMRGSVGAALVKARRAVHVLR
                     SPDWADYVHYGDAEFRLKVS"
     gene            complement(5347..6216)
                     /locus_tag="VEW08_01665"
     CDS             complement(5347..6216)
                     /locus_tag="VEW08_01665"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ39463.1"
                     /translation="MIPQPFDLRAPDGRGLAAHWFTASQRRGVIVMNGATGFPQLFYF
                     KLAHYASERGYDVLLYDYRGMGFSARDKTAMEKARMSDWGLLDMRAALDAGAERAQGQ
                     PVVTFGHSVGGQFLGLLRNHALACAHVQIATSTGYWRWEHAPFRYLAWWFWRVHGPMM
                     LALMGYIPTGGGWAGLPLPRGVYEEWRRWCLRPEHFGPDLATYLADNYFTDIRAPLLS
                     VGFTDDPIATRRTVGELNKFFPNVQRESRWFTPAEAGSKRIGHEGFFASRHRDTLWRP
                     VIDWIDGKLGVAA"
     gene            complement(6213..>7070)
                     /locus_tag="VEW08_01670"
     CDS             complement(6213..>7070)
                     /locus_tag="VEW08_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HYJ39464.1"
                     /translation="AVADTRHFGRAAERSFVSQPTLSAQLKKLEEYLGVQLIERAPKR
                     VSLTVAGEEIVERARRIVEASEEIIELAKGHTDPLSGRLKLALLPTIGPYLLPNVAGK
                     LRKQLPRLELMLYEYQTDVMLEKLHSGEIDVGILALPVPMDGLDSYELYKEPFTVAMP
                     ANHRLAGRSNIKVADLHDETLLLLEDGHCLRDQALEICSSTGVHEKQDFRATSLETLR
                     QMVAAGVGITLLPELAGRGAYGAARGVTIKPFAKPVPTRTIGAIWRKSSARRAVILAL
                     AKHIEAHAL"
BASE COUNT         1241 a         2289 c         2338 g         1202 t
ORIGIN      
        1 tgtcgatgat gatgatctgc aggatgatgg cgccgaagct ggccttcacg ctctttgaag
       61 catggccttc ctcaccttcg gtgagctgat gaatttccat cgtggacttc cacaccagaa
      121 acacaccgcc ggcgatgagg atgagatcgc gcccggagat gtccgtgccg gtgacggtga
      181 acagcggcgc ggtgaggccc acgatccagg acagcaccat cagcaggccg atgcgcatga
      241 acatggcgag gaacaggccg atgcggcgcg ccttttcgcg ctgcacgcgt tcgagcttgt
      301 cgaccaggat ggagatgaaa atgatgttgt cgatgccgag caccagctcg agcgccgtca
      361 acgtgaaaaa tgcgatccat acctgcggat cggacaggag ggcaacgata gcttccatga
      421 gtgccgccat attagccgag gaccgcggac gcggtaccct tcgtaaccat gatcaaagtc
      481 atcaagaatc tcattgcctc attcctgttc ctggcgcttt ccgcgcaagc cggagcgcgg
      541 ctggcgtctg gcgaagttca tgtctggcag gtccaggaaa tcaccttcgc ggcggcacgc
      601 gactattcga atccctatgc ggacgtcgaa ctctgggtgg agctggaagg cccggatttt
      661 tttcgccggc ggatatacgg cttctgggac ggcggccgca cctacaaggt tcgcctggtt
      721 gccacacatc cgggtgaatg gacctggcgg gcgggctcga gtcgcgacga tcccgggctc
      781 gcgggcgctg gaaaattccg cgcggtcgcc tggagtgagg ccgagctcgc ggaaaatccg
      841 aatcgacgcg gcttcgtgcg caacagtgaa aacgggcatg cgctgcgtta cgccgacggc
      901 tcgccgttct ttctcacggg cgacacctgg ctcgccgcct cgacgtggag gttgccgttc
      961 aaaggcacgc gtgctgccgc ggactacgtg cctgccgaag gcatcagctt cgaggaggcg
     1021 gtggcctggc gtaaacgcca gggctacaac tccatctcat tcatctccgc gtttccgaac
     1081 tgggcggctg acgaacacgg cgcgaccttt gccaacaagg acggcgtcta tttgcgtaat
     1141 gcctgggaga aatttggcca ctgggcgccg aacgcgaaga tctccacggc cgacggcgcc
     1201 accaccaccg ccaaggacat gcacgacgaa gcgggcaacc ggcctttcga agtgttcgcc
     1261 gaccgtgaag ggctcgcgaa cttcgatgcg ctgaatccga aatatttcgc cagcctcgat
     1321 cgcaagatgc agcacctgtc cgatcagggg ttcgttccgt ttctcgagac catacggcgc
     1381 gacaacgcgc cttcctggaa gcgctacttc gatttcaacg tgtcgtatgc gcgcttcgtg
     1441 caatacctgg tcgcacgtta tggcgcgtac aacctcgtgt tcagcggaat tcatctcgac
     1501 tggattccaa aggatttcag tctgacggcg gacgagttca acgacgcgct cacctggcac
     1561 taccgcaagt acgggccgct gcctttcggg cagccataca ccgcgttgat cgaccgttcg
     1621 acgctcaagg ctttcggaac cggcgcgcgg gcaccgtggc tcacgatgca caccgtggga
     1681 aacaatccgc gcaaccacgc tatctacgag tccatcgaag aactgttcgg gctcaccccg
     1741 ggcatgccgg cagccaatct cgagccttac tacacggggt ggaaccactc catcaatcga
     1801 cccggcggcg agatcccgga cgagaactcc gcgcgcgaca actatttcgc gcgcgcgatg
     1861 atgtacggct ccgtgctgtc tggcgcgctt gccggacacg tgcatggaac cgcggcctac
     1921 gatgtcacga gtaccggcga gccggctggc tggcggccac acatctggac cgcgctgcgc
     1981 tacgaatccg gcgcacagat gcagcatttg cgagcgttcg tgctctcgca agggagtctg
     2041 taccagcggt tggaggtggc ttcccgcgat ctcgatccgc gcagcatccc cggcgcggca
     2101 gccgatggac tcgacggatg gtcgttcctc atgcgtacgc cggcgaaaga tttcgcactg
     2161 gcgtacttcg agaacaaggc catgcgcgcg cgcatgaaag gtttcacccc tgccgccaag
     2221 tatcaatgga cctggttcga tccgcgcacc ggccaatggg ggcgggcggt cagcgtgaaa
     2281 gccgacgcgt cgggcgtcat ctcgacgcca ccattcgcag ccggcggcaa ccaggccacg
     2341 aacgatgtgg ccgcgaagat tgtcagggtt gtcaggagac cttgagccgg aactccgcat
     2401 ctccgtagtg cacgtagtcc gcccagtccg gcgaccggag aacgtgcacc gcgcggcgcg
     2461 ccttgaccag cgccgcgccg acgctcccgc gcatcagctc gcggtagaac accgtcgcga
     2521 aagccgtcgc ggatgcgtct tcgacaggcc agtgtgtgcc gacgtagtgc gcgagaccgg
     2581 cgcgcagcaa ggtttcggcg aggctcaggt tcctggcaat gccctggccc ctttgtgtgg
     2641 cactgcgctt gggcgtcgca ttgcgccgca ggcgcgcgga ttcacacgcg ttgaacacca
     2701 cgaggggcgg caaccttccg agggcggaca aatgcgcgcc ggtgagcacg ccgtcagcga
     2761 gcgcaagacc gccgtcgcct ggaagcactt catcgaagga agcgtgaccg gcgtagtgaa
     2821 tgacgtcgta tcggccggat tcgaactccg cggtcacgcg cgcgagcgtc gcggcgttgc
     2881 cggcgacttc ggtgaggcgc accgtcttgc cgcgcccgag gaggtgcgca atgcgttcgc
     2941 gctcgctctc ggcgccgggg agatcaccgg tcggattcgc gatcagcagc acaccgagtt
     3001 cgcgctgctc gcgccgcccg gtgtcgaatc gtgccggcac caggtcggcg gttgcgtaac
     3061 gccgcgacag tccgccgtcg agcgccggga cccatccgcg aatgttgagt gcttcccagg
     3121 gaatgcggct gctcgcggca tcgtgtacca ccgacagcgc ctggccgcgc gcggcataca
     3181 gcgcgctcgc cagcgacgga tgcagcgtga gggctccgag cttcccgccg agtgacttca
     3241 cgcgcgcggg agtcagggcc tcgccctgga attcccggac cagccggtca agcgcctgcg
     3301 cggcaaactc ctggctctgc gagaaaatcg cggcagagct gccgccggtg agcagcgagg
     3361 cgcgccacgt ctcgcgtttg ccccgccgtg actccgccgc gacgatgaca tgagccgtac
     3421 ccggtacgcg tcgcacgcca gtcgcggcgg acttttgcga cggcgcgagc gggcgcaatc
     3481 gcaacatgcc ggcgggacgt gacttcacga agtcccggag cctggcatgc actgtctggg
     3541 cgtccgcacg cgtcagcacg tggagatcca ctcgcgagat gccagcctcg cccgcggcgc
     3601 gtgcgcgcaa cagtccccgg agctgagcgg cgaatgaatc agcgggttcg atgcccgcgg
     3661 cggcacccca ggcaacggtg gcgatcgacc ggtattgcgg cgcctcggcg aaccgcgcga
     3721 ggttttccgc cgcgtgttcg atgacgtcga ggctcaggcg gtcgaatcgt ccgaggccaa
     3781 ccagcagaaa cgacgtgcgt ggcccgcggg tctttttcac caggctgcgc ggtatcggcg
     3841 tgacgtgacc ggcatcgcca ctaactacac ggtgctggag ccagtcaccg atgatcccgc
     3901 caaggcgctg atcgatgtcc gccgcggcgc cgcttgccgg tacgccgcgc aacaccgcga
     3961 cggccgtggc cgcggcgcgg gctttggcca cgtcgccaac ccgcacattg atggagagcg
     4021 gttgaatctt gccggcacgc tgcgggcgtt gatgtacgcg tccgcgcggc gcttcgttga
     4081 gtgtgtccag gaacagccgc cgctgttccg gcgtcatgtg cggccagtcg atcttcccct
     4141 gatattgcgc gcgcagttcg gtgtcgcgta cccgggtcag tgaaccgcgc ttcaccggcg
     4201 gtgcggcagc gaaacgttgc gtggcaccgg tttcgagcag gtcgatggtg ccgtcgatga
     4261 cgcggtcgga cagggtcatg tcgctgtgcc cgcattccac gtagctgtgg cgcgcgccgg
     4321 cgagccgcgc gagctcgatg ggtacggtgc cgtcgccctg caggctgtat tcgtactcga
     4381 aatcaccatc gcgcagtgcg acgccggtgg cggtggtgcg gttgcagccc accaccatgt
     4441 tgaatcgcgc gtcggcgggc gccatgcgct gctcgaggcc ggacgccgag cgaagcaggg
     4501 ctgcgtcggg acccggaccc ttcgccggcc ataccgccgc gtcgaacagg tcgagatcgc
     4561 tcacgctctt gttcgccggc agcaactcgt gcaatccggg gaaactcgcg aacacattgc
     4621 gtgccaggta ttccgcgtca tgccgcaggt cgagcattgc gatcttgcgc accaccgagt
     4681 aggtgccgcg cagcgcctgg accgcggcga gcgacccgga gttgggagtg ccgagcatga
     4741 tgagttgcga gacgcgcgac ccagcggcat gcgtgagtgc cgcgcgcgcc accaggccac
     4801 ccatgctgtg tccaatgagc gccacctcgt ctcggccgtc ggccgcgatg cgtgccgcga
     4861 gctcggtgcc gagagtcgca atgtcgcgcc gccagtcata gtcgagcagc accgcgtcga
     4921 aaccggcctt gcgcagcgac agcgtgaact tgagatagct gtagttcatc gcgccgagcg
     4981 ccacgatgcg cgacgcgtcg ttcagtcgca gctccgtcaa acggccgaag gcgatatcga
     5041 tgggatccag ccaaagaatg tcattgggtc gcttgccgcc gcgaatgaac ccgagctgcg
     5101 aacccatgat gcccggcaga acatagacgc ggcgccgcgg tgtggcgcgc gtcttgcgtt
     5161 tgcgcgtggt ggcccgcgcg agcgaagcga gctccgcgtg cagctccgcc ccgaagtatt
     5221 cgaccagccc ctcgttcgga gtacccgacg ccagcagtgc cacgagcgtc gcctcgtcga
     5281 gcgccagccg gtcgtagggc gtgatgtgct cggcaggtgt gccgggattg ctcgtgtggg
     5341 aatggctcat gcggccacgc ccagtttccc gtctatccag tcgatgaccg gccgccacag
     5401 cgtgtcgcgg tgacgcgagg cgaagaagcc ttcgtgcccg atgcgcttcg agcccgcctc
     5461 ggcaggcgtg aaccagcgtg attcacgctg tacgttcggg aagaacttgt tgagttcacc
     5521 gacagtgcgc cgcgtggcga tcgggtcatc ggtgaacccc acggagagca ggggcgcccg
     5581 gatgtcggtg aaatagttat cggcgagata ggtggcgagg tcggggccga agtgctccgg
     5641 ccgcaggcac catcgccgcc actcttcata tacaccgcgc ggcaacggca ggcccgccca
     5701 accaccgccg gtcgggatgt atcccatgag cgcgagcatc atcggaccat gcacgcgcca
     5761 gaaccaccag gcgaggtagc ggaacggcgc atgttcccag cgccagtaac cggtcgacgt
     5821 ggcgatctgc acgtgcgcac acgcgagcgc atgattgcgc agcaaaccga gaaactggcc
     5881 accgacactg tgcccgaaag tcaccaccgg ctgaccctgg gcgcgttccg cgccggcgtc
     5941 cagcgccgcg cgcatgtcga gcaggcccca gtcgctcatg cgcgccttct ccatggcggt
     6001 tttgtcgcgc gcggaaaatc ccataccgcg atagtcgtac agcaacacgt cgtagccacg
     6061 ttcggaggcg tagtgcgcca acttgaaata gaacaactgc ggaaagcccg tggcaccgtt
     6121 catcacgatg acgccgcgtc gttgcgaggc ggtgaaccaa tgcgcggcaa gcccgcgccc
     6181 atccggtgca cgcagatcga acggctgggg aatcacagcg cgtgcgcctc gatgtgcttc
     6241 gcgagcgcga gaatgacggc gcgacgtgcg ctcgatttgc gccagattgc gccgatggtg
     6301 cgggtcggca ccggtttggc gaacggtttg atggtgactc cgcgcgccgc gccataggcg
     6361 ccacggccgg cgagttccgg cagcaacgtg attccgacac ccgcggccac catctggcga
     6421 agcgtctcga ggctcgtggc gcggaaatcc tgtttctcgt gcacgccggt gctcgaacag
     6481 atttcgagcg cctggtcgcg caggcagtgg ccgtcttcga ggagcagcag tgtttcgtcg
     6541 tgcaggtcgg ccaccttgat gttcgagcgc ccggccagcc ggtggttcgc cggcatggcg
     6601 acggtgaacg gctctttgta cagctcgtag gaatcgaggc catccatggg caccgggagt
     6661 gccaggattc cgacatcgat ttctcccgaa tgcagtttct cgagcatcac gtccgtctga
     6721 tactcgtaga gcatgagctc gagccgcggc agctgcttgc gcagcttgcc ggcgacgttc
     6781 ggaagtagat aggggccgat ggtgggcagc agcgccagct tgagccgccc ggacagcggg
     6841 tcggtgtggc ccttggcgag ctcgatgatc tcctcgctgg cctcgacgat gcggcgcgcg
     6901 cgctcgacga tctcctcgcc ggccacggtg agcgacacgc gcttcggtgc gcgctcgatg
     6961 agctgcacgc ccaggtattc ctcgagcttc ttgagctggg ccgagagcgt gggctggcta
     7021 acgaagctgc gttccgccgc gcgcccgaag tgacgggtat ccgccacggc
//
LOCUS       DASQJS010000042         4132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1281296, whole genome shotgun sequence.
ACCESSION   DASQJS010000042 DASQJS010000000
VERSION     DASQJS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281296"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VEW08_01675"
     CDS             <1..279
                     /locus_tag="VEW08_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019407031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HYJ39465.1"
                     /translation="SDAEPLRFAHNERLRIALVNDTMMTHPIHLHGMWSEITDASGKF
                     VARKHTVTVQPGQRLTCEVTADALGSWAYHCHLLYHMEAGMFREVVVA"
     gene            276..1061
                     /locus_tag="VEW08_01680"
     CDS             276..1061
                     /locus_tag="VEW08_01680"
                     /inference="COORDINATES: protein motif:HMM:NF017119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HYJ39466.1"
                     /translation="MRRACAVFAMVALVAQAFGEEPGQSEREHVPPDPPQTRVHAMPY
                     HEMAAMMGMDDRKRFGKVMIDRVEWRDGDSFSWDATAWYGGDFHKLWVETEGEHTDGT
                     TRDARIEVLWDRIATAWWSTRLGMRWDVGDGPTQSWVAFGVAGLAPGFVELEAMAYVG
                     DGGRTALRLSTEYDLLLTQRLVLQPALELNAYGEDDPGRLIGAGLSDLELGLRLRYEF
                     RREVAPYVGVNWLKRFGDSANFAEAAGEESDEFAVVAGIRLWF"
     gene            complement(1120..2034)
                     /locus_tag="VEW08_01685"
     CDS             complement(1120..2034)
                     /locus_tag="VEW08_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HYJ39467.1"
                     /translation="MRSSCVLVVSGLLAACGGGQPPVAEVQPVESATPATATADTPVS
                     EPAKVALVFRVMREGGGGIDATINEIDPQGQVRFYATVPGTGEKALDKPCDAGQRFQA
                     EPRVAAFLRGPTQECADRVEFMLYSTQATLQFIKRGDDASRGGDLLVAQSNYGTAADR
                     LQFAEPDKARQLRVLSTVAAGRALGVTRPVQGAGGSEQPTPEFKDRVRVFQRQNGIEA
                     TGDLDARTRETLGRMQLRGDAVVVPPAASSFSPAGAAAVATAPALVVTPQAVTPAVEV
                     RVSTAEILATPASPKTAAMIHANRAKVK"
     gene            complement(2027..2650)
                     /locus_tag="VEW08_01690"
     CDS             complement(2027..2650)
                     /locus_tag="VEW08_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39468.1"
                     /translation="MGWWRRFFNGLLSKDKSLATGLILAFMAWTLMRLVDGIAANGTI
                     EYAIDNTPATLADGRPGHLVRVTLTNLSADTALTNLKASIAAPTADIVFSADPRDRSC
                     AFEPPGWGGQPTCDAFASGFDFLAPMIVAGTHVQFEVKYTRPDGVATLPIVRIRPETT
                     KFRLVEPGFSTFVARNQVGLLLALLMLALVMFFLSVAAGVPKGEPHA"
     gene            complement(2767..>4132)
                     /locus_tag="VEW08_01695"
     CDS             complement(2767..>4132)
                     /locus_tag="VEW08_01695"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HYJ39469.1"
                     /translation="IFENKEGLQLEESHGYDRRVIDYIQRASASGLYEIRGLGAKRHL
                     PSFDDLVFLGASLSRYPLEGYREKCSTRTVLGTRHATKPIELATPITIAGMSFGALSA
                     RVKEALGRAATAVGTSTTTGDGGMTKEERESSKILVYQCLPSRYGFNPDDVRRADAIE
                     IVIGQGAKPGGGGMLLGQKVNPRVAAMRTLPAGVDQRSASRHPDWTGPDDLVIKINEL
                     REITDWEKPIYVKVGATRVNHDVKLAIHAGADVIVVDGMQGGTAATQTVFIEHVGIPT
                     LAAVRQAVEAIDDLNMRGKVQLIVSGGIRTGADVAKALAMGADAVAIGQAVLMALGCN
                     SDSYLAGDQHVSAVEDYARLGTAPGFCHHCHTGRCPVGVTTQDPVLEQRLEPASGARR
                     LTNYLKTLNMELTTLARACGKQDVHHLEREDLVALTVEAAAMANLPLAGTNWVPGGES
                     GEKR"
BASE COUNT          703 a         1324 c         1378 g          727 t
ORIGIN      
        1 tcggacgccg aaccgctgcg atttgcgcac aacgagcgcc tgcgtatcgc tctcgtgaac
       61 gacaccatga tgacgcatcc catccacctg catggcatgt ggagcgagat cacggacgcc
      121 agtggcaagt tcgtggcccg caagcacacg gtgacggtgc agccagggca gcggctcacc
      181 tgcgaagtca cggccgacgc gctgggtagt tgggcgtatc actgccacct gctctatcac
      241 atggaagcgg gcatgttccg cgaagtggtg gtggcatgag acgtgcctgc gcagttttcg
      301 cgatggtcgc gcttgtggcg caggcgttcg gcgaagagcc gggccagagc gaacgcgaac
      361 acgtcccacc cgacccgccg caaactcgcg tgcacgccat gccctatcac gaaatggccg
      421 ccatgatggg catggacgat cgcaagcggt tcggcaaggt catgatcgac cgagtcgaat
      481 ggcgggacgg cgactccttt tcatgggatg ccacggcctg gtacggcggg gatttccaca
      541 agctttgggt cgagaccgag ggcgagcata cggacggcac gacgcgcgat gcgcgcatcg
      601 aggtgctctg ggaccgcatt gccaccgcat ggtggagcac tcgcctggga atgcgctggg
      661 atgtgggtga tggcccaacg cagagctggg tggcgttcgg cgtcgccgga ctcgcgccgg
      721 gattcgtcga gctcgaagcc atggcctacg tgggtgatgg cgggcgcact gcgctacgtc
      781 tatctaccga atacgacctg ctgctgacgc agcggctggt gctgcagcct gcgctggagt
      841 tgaacgccta cggcgaggac gatcccgggc ggctgatcgg cgcgggcttg tcggacctgg
      901 aattggggtt gcgattgcgc tacgaattcc gtcgcgaagt cgcaccgtat gtgggcgtga
      961 actggttgaa gcgttttggt gacagcgcaa acttcgcaga ggccgccggg gaagaatccg
     1021 acgaattcgc ggtggttgcc ggaattcgtc tctggttctg aaagggaaac gagggcgggg
     1081 ctgctcgctg gagcccatct gtcaaacctc caaccccgtt tacttcacct tcgcgcggtt
     1141 ggcgtgaatc atcgccgcgg tttttggcga tgcgggagtc gcgagaattt ccgcggtgct
     1201 cacgcgcacc tccaccgccg gcgtcaccgc ctgcggggtg acgacgagtg ccggcgctgt
     1261 ggcaacggcg gcagcgccgg ccggactgaa cgatgacgca gcaggcggca ccaccaccgc
     1321 atcgccacgc aactgcatgc ggccgagggt ttcgcgcgtg cgcgcatcga gatcgccggt
     1381 ggcctcgatg ccattctgcc tctggaacac acgcacgcga tccttgaact cgggcgtggg
     1441 ttgttcgctg ccacccgccc cttgcacggg ccgcgtgacg ccgagtgcgc gaccggccgc
     1501 caccgtggac agcacgcgca actgccgcgc cttgtccggc tcggcgaatt gcaggcgatc
     1561 cgccgcggta ccgtagttgg actgcgcgac cagcaggtcg ccgccccggg aggcatcgtc
     1621 gccacgcttg atgaactgca gcgtagcctg ggtgctgtag agcatgaact ccacgcgatc
     1681 ggcgcactct tgtgtcgggc cgcgcaggaa ggccgcgacc ctgggctcgg cctggaacct
     1741 ctgccccgca tcgcagggct tgtcgagcgc cttttcaccc gtgcccggca ccgtggcgta
     1801 gaagcgtacc tggccttgcg gatcaatctc gttgatcgtg gcgtcgatgc cgccgccccc
     1861 ttcgcgcatg acgcgaaata ccagcgccac tttcgcgggc tcagacaccg gtgtgtccgc
     1921 ggtcgccgtt gcgggtgtcg cgctctcgac cggctgcacc tccgcaaccg ggggttggcc
     1981 tccaccgcag gccgccagca atccgctgac gacgagaaca catgagctac gcatgaggct
     2041 ctcctttggg aactccggcg gcgacactca ggaagaacat gaccagcgcg agcatcagca
     2101 atgcgagcag cagcccgacc tggttgcgcg cgacaaacgt cgagaatccg ggttcgacga
     2161 gccggaactt cgtggtctcc ggcctgatgc gcacgatggg caaggtggcg acgccatccg
     2221 ggcgcgtgta tttcacttcg aattgaacgt gcgtgccggc cacgatcatg ggagcgagaa
     2281 aatcgaagcc gctggcgaac gcatcgcagg tgggctggcc accccacccg ggcggctcga
     2341 aggcgcagct tcgatctctc ggatcggcgc tgaagacgat gtcggccgtg ggcgcggcga
     2401 tcgacgcctt gagattcgtg agtgcggtgt ccgccgatag attggtcagc gtcacgcgaa
     2461 ccagatggcc gggacggccg tcggccaggg tcgccggcgt gttgtcgatg gcgtattcga
     2521 tcgtgccgtt ggcggcgatg ccgtccacca ggcgcatcag cgtccaggcc atgaaggcga
     2581 gaatgaggcc ggttgccagg gacttgtcct tggacagcaa gccgttgaag aaacgtcgcc
     2641 accaacccat gcgggatttt catccagcgc ccggcgtgct cacgccgtcg cccgagggtc
     2701 agcgggcaaa agtgtgaaaa ggtgccgggt ggggatagtg gggagaaccc ttgctaccta
     2761 cttccttcag cgcttctccc cactttcccc acccggcacc cagttcgtac cggccaacgg
     2821 caggttagcc atggcggcgg cttcgacggt gagcgccacc aggtcttcgc gttcgaggtg
     2881 gtgaacgtcc tgcttgccgc aggcgcgcgc cagcgtggtg agctccatat tgagcgtttt
     2941 caggtagtta gtgagccgcc gggcgccgct ggccggctcc aggcgctgct cgagcaccgg
     3001 atcctgggtg gtcacgccta ccgggcagcg gccggtgtgg cagtgatggc agaacccggg
     3061 ggcagtcccg agtctggcgt aatcctccac ggcggaaaca tgctggtcgc ccgccagata
     3121 gctgtcgctg ttgcagccga gggccatgag cacggcctgg ccgatggcga ccgcatcggc
     3181 acccatcgcc aacgccttgg ccacatcggc gcccgtgcgg atgccgccgg agacgatcaa
     3241 ctgcaccttg ccgcgcatgt tgagatcgtc aatggcttcc acggcctggc gcacggcggc
     3301 cagcgtgggg attccgacgt gctcgatgaa caccgtctgc gtcgcggccg tgccgccctg
     3361 catgccatcg acgacgatca catcggcgcc ggcgtgaatc gccagcttca cgtcatggtt
     3421 cacgcgcgtg gcacccacct tcacgtagat gggcttctcc caatccgtga tctcgcgcag
     3481 ctcgttgatc ttgatgacga ggtcgtcggg gccggtccag tccgggtggc gcgaggccga
     3541 gcgctgatcc actcccgcgg gcagcgtacg catcgccgcc acgcgcggat tgactttctg
     3601 ccctaacaac atgccaccgc cgccgggctt ggcgccctgg ccgatgacaa tttcgatggc
     3661 atcggcgcgc cgcacgtcat cgggattgaa tccataacgc gacggcaggc attggtacac
     3721 gaggatcttc gagctctcgc gctcctcctt tgtcatgccg ccgtcacccg tggtcgtcga
     3781 ggtacccacc gccgtcgcag cgcggccgag cgcctctttc acgcgcgccg acagcgcgcc
     3841 gaagctcatg cccgcaatgg tgatgggggt ggcgagctcg atgggcttcg tggcatggcg
     3901 cgttccgagc acggttcgtg tcgaacattt ctcgcgataa ccctcgagcg gataacgcga
     3961 cagagacgcg cccagaaaaa cgagatcgtc gaaagacggc aggtggcgct tggcgccgag
     4021 cccccgtatt tcatacagac ctgaggccga cgcgcgctgg atgtagtcga tgacgcgccg
     4081 gtcgtagcca tgcgactctt cgagctgcag cccttcctta ttctcaaaga tt
//
LOCUS       DASQJS010000043        18054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2536548, whole genome shotgun sequence.
ACCESSION   DASQJS010000043 DASQJS010000000
VERSION     DASQJS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18054
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2536548"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VEW08_01700"
     CDS             complement(<1..228)
                     /locus_tag="VEW08_01700"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HYJ39470.1"
                     /translation="MSKYQLAQLNIAALKAPLDSPELKDFVDNLDRINELAEKSPGFV
                     WRLKGDGNDATSLRPLGENIIINMSVWRDVDA"
     gene            complement(254..1012)
                     /locus_tag="VEW08_01705"
     CDS             complement(254..1012)
                     /locus_tag="VEW08_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39471.1"
                     /translation="MLLTVALFAGMLIALEVGRRVGVARLAHDPEGLTKGAAAAEGAV
                     FALLGLLLAFTFSGAASRFEERRWFINTEANAIGTAYLRLDLLPEETRAPLRDLFRRY
                     VDLRASVFKNAKSQEEVEASIVKGSELQAEIWNQVMAACRLPAASPQATMLLTPALNE
                     MIDITATRQMATRNHPPVIIFVLLALLSLVGALLVGYGTSANKDRSWFHHVTFAFVIT
                     LAIYVIVDLEYPRLGLIKVDSADQALLDVGKSMR"
     gene            complement(1071..1715)
                     /locus_tag="VEW08_01710"
     CDS             complement(1071..1715)
                     /locus_tag="VEW08_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39472.1"
                     /translation="MRTILLALAMLMPLASANADCDPPGLFRIVTANGSPGIPKDSFA
                     SRPKVTYRAGNGRVRGEEMADTAGGIHQLSVINWPDMWVVNLLDKTGEHVVDDSPKSD
                     VTIAALGLEPSEGLPSAWASLEFGCEWEFFAANKAKQVPTEKHDMIKHQLTDGAWRVT
                     MITAKDSQVPWALMLSRDNNVVYAIRYLSYEHRDQVDPALFAKPDGIRFKDPLK"
     gene            complement(1730..2098)
                     /locus_tag="VEW08_01715"
     CDS             complement(1730..2098)
                     /locus_tag="VEW08_01715"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HYJ39473.1"
                     /translation="MKPRDVDSYISALPKEIQPLLKKVRRAIRGAAPNATEVISYNMP
                     AYRQQGMLVYFAGCKSHIGLYPPVRGNEALQKAAAKYAGPKGNLKFPTDQPLPLTLIN
                     RIVRHNAKQNAAKAMARKKK"
     gene            complement(2100..3242)
                     /locus_tag="VEW08_01720"
     CDS             complement(2100..3242)
                     /locus_tag="VEW08_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ39474.1"
                     /translation="MNPYIIPWMDDDLEVFRDTVVRFIDTEMVPHDARWREQHHVDRE
                     TWRKAGDTGLLLLDVPAEYGGGGGDFRHEAVMYTELARRGIASFGQGVHSMCSHYVLN
                     HGTEEQKRRFLPRLAAGELIGAICMTEPGTGSDLKGIRTRAVKQGDHYVVDGGKTFIT
                     NGFLAGLLMLVVRTDADRSKGLSILMVETKDLPGYRVGRVLDKMGMHGQDTSELFFEG
                     VKVPAENLLGGAEGQGMHQLMHDLPYERTVIAVGAVGAMEGGIDETLKYVRERKAFGQ
                     PVWSYQNTRFKLAELASIARIARVFSDRCVVDLVAGKLDTETASMAKWWCSDMQQQVL
                     DECVQLHGGYGYMNEYLVCRLFADSRVQRIYGGTNEIMKEVIARGL"
     gene            complement(3290..3712)
                     /locus_tag="VEW08_01725"
     CDS             complement(3290..3712)
                     /locus_tag="VEW08_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39475.1"
                     /translation="MLITGSCHCGNIRFTLDWLPDPTEIPARACGCSFCVKHGGVWTS
                     SPSGTLRIKVRRPERVTPYTFGTRTAEFHVCTDCGIVPVVTSRIAGILYAVVSVNAFD
                     NVGLERLRRIPASFDGESEGDRLARRQRNWIGNVEFGP"
     gene            complement(3722..6088)
                     /locus_tag="VEW08_01730"
     CDS             complement(3722..6088)
                     /locus_tag="VEW08_01730"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HYJ39476.1"
                     /translation="MRHFCLTAVAGLLALASFAQAQNANVLWYGQPATDWEKEALPIG
                     NGRIGAMVFGGVDSERLQISEKSLWTGGPGSEGGYDFGLPADSQVELMRSIGKQLTDG
                     AQLKPEDVAKQLGRKMHNYGDYQSFGDFIIERESDGTPVSGYRRELDIDTATAKVSFK
                     QGDAGFRREYFVSYPDQVLVGRWYGTSLQKLRVRFVVPDNRSVTIRAEGTRIMISGAL
                     KSNGLQYAAELRVIPECGSIKADGDSLRIESECQITFLMAARTNYRMRYPDYREAGAD
                     PAAAVAKDAGIQGKDRYAQYFARHRDDHQELFRRVALRLEAPVSKLPTDKLLAQYGTG
                     NAALDRQLEQLYFQYGRYLLIASSREGSLPANLQGVWNDKATPPWNADYHVNINLQMN
                     YWPAEVANLTETTPPLFDFVDHLVPPGKLAAQRYFGANGWTMFLNTNAWGYVGPIDWP
                     TAFWQPEASAWLAHHYYEHYLFSQDLEFLKKRAWPVMKGAAEFWLDALVADPRDGTLI
                     VSPSYSPEHGPFTAGAAMSQQIVADLFVNTSGAAQKVGDKVFAKRVDDALAKLDRGLR
                     VGKWGQLQEWKADLDDARDDHRHVSQLFALHPGHAIDPVKNPELLAAARATLDARGDA
                     STGWSRAWKVNFWARLRDGDRAHKLLEGLIRDSTLPNLWDTHPPFQIDGNFGATAGIV
                     EMLLQSQNGELHILPALPKAWGKGSVKGIRARGDITVDIQWDSCGPVQLDLTTGHAGA
                     VVLRSTLFEKGFDGTVKTEGALASRKFMAKRGGEYTFTRAPSVACGAD"
     gene            complement(6209..8884)
                     /locus_tag="VEW08_01735"
     CDS             complement(6209..8884)
                     /locus_tag="VEW08_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ39477.1"
                     /translation="MAQAPEHPGDLTASDVFATGVWSARGGKPALGVDIACTKISIDL
                     QNMRRGGIESTIRDSLNSLRDSSAVDTAFVWLFSPDGGTIEEVYSAHGALAQCRVEAW
                     RSASIDTYPWLKGRLDHLRLSEIRDSSAPRREQIIESQRLAELQIGSVLMIAFRVGGV
                     PAGILGLANALPRGAWDVNLQLLLKLLGNSLGAGLERLRVEGRLHKLEERLELAQGAA
                     NDGLWDFDIESNEVHFSPRWKQMLGYPVDAQLDSPDWRSLVHPEDMSRVQSAIRDHVA
                     GKSPIFESVHRMKHRNGDWRWVVSRAKARVDEHGRLLRLVGVELDITERKLYEEALFR
                     EKESAQITLQSIGDGVVTTDANSTVDYINPVAEQLTGWRLEDAMGRPIEEIYRAFHEE
                     TCEPLENPLSVSIRRTRPIKSVRPMLLIRRDGNELYVESTAAPIRDGAGAVSGGVLVF
                     HDVTESRELNRRLSYHASHDLLTGLVNRREFESRVERALKSAKARESSYALCYLDIDQ
                     FKIINDTCGHSAGDVLLGQVGALLKSKVRWRDTLARLGGDEFGILLEACSLDEALRTA
                     EVLREAVRNFRFTWEDRVFRLGASVGVVPIAADNEDVASIISAADSACAAAKEGGRNR
                     VHSFAENDIELMRRRREMQWAARINAALEEGRFELYRMQIQPLQRAEPGQHYELLLRM
                     RDESGRMVSPDNFIAAAERYGLTPAIDRWVIENALRWLVSEADEREKLAMCSINLSGQ
                     SFGDDKFLPFVIEQFQKSGIDASKICFEITETAAVASFSQANRFIQALKELGCRFALD
                     DFGTGLSSFGYLKHFPVDFLKIDGSFVREILHDPIDREMVRSINEIGHLTGKKTIAEF
                     AENAEIIQMLTSLGVDYAQGYGIAQPQRVLKAVNA"
     gene            complement(9065..11059)
                     /locus_tag="VEW08_01740"
     CDS             complement(9065..11059)
                     /locus_tag="VEW08_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYJ39478.1"
                     /translation="MPDIGLLQGGAPRPRVVPPLKVPAAEEGVPAGAPVAPDREIAAP
                     FLAPIDSAIAVVTAVVSAPSAAGAGLAALPPESAAPVASPGGKGASSIPTQPAGLAPI
                     RARDLLWLALALAIIIGTGLGVRDPWPADEPRFAALARDMALSHEWLFPRVGGDLYQD
                     KPPLYFWLLAICYTLFGSVKASFLIPSFLAAGGTLFLIYDFGRRTVSREAGLAAALIS
                     VCTLQFLMVMRGAQIDATLCFLTTFSLYALLRHLLLGPAWGWYFIGGFVAGLGVFTKG
                     VGFLPVLLLIPYFSLRAFRWNGLPPIDAGAVGWRWWLAPLAMLMAISLWFVPMLVAAA
                     SGSAEYAAYRDEILFKQTVGRYAAAWHHVKAWYYFILEVIPALWLPWSLLLIWLAPRF
                     KAAFHERNARVWLPLFWVLLVLAFFSASPGKRGIYILPALPALAIAALPYLESLLTRA
                     GVRRAGFVLGGIFFVAAAVLAIAYTVDMKFAREAIAAANLGGVTSLYVYLALCGAGLA
                     FAALRAPLAAWPVAIGSLAIVFSYLIAPAMNGQRSGKDFTRAVLAQLKPGEFLALVAY
                     KEQFLLYLDQPTINFGHRRWLEGPQESYDAAQWLNTAPNRVLLVPESTMKKYPCFLVS
                     VTPAGRTSDEEWFLVRAPAERSCAEQGDSSRAIAYAPTGH"
     gene            complement(11052..11363)
                     /locus_tag="VEW08_01745"
     CDS             complement(11052..11363)
                     /locus_tag="VEW08_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HYJ39479.1"
                     /translation="MMSGVIGVYFGVVLTPWKLVGFLGALMFAGRWVVQAWATKRAGR
                     PMIPRSFWIISLLGSAMVTSYFIWGKNDSVGVLTNLLPASVAFYNLVMDIRSKRAGQN
                     A"
     gene            complement(11382..12107)
                     /locus_tag="VEW08_01750"
     CDS             complement(11382..12107)
                     /locus_tag="VEW08_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015066033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYJ39480.1"
                     /translation="MSIEFSVVVPVCNEAENVEPLAREIDAALAGRSYEMIFIDDGST
                     DDTASNLLKLKNSLSALRVLRHSFRSGQSAAVATGVRAARAPWVATLDGDGQNDPADI
                     PKLIAARDSAEGRGVQLFMGNRTASRKDTAFRRLQSRIANGVRGGLLGDGTPDTGCGI
                     KLFSRDVFMDLPRFDHMHRFLPALFQRHGARVISVPVSHRERTRGTSKYGMLNRLWVG
                     IVDICGVMWLRRRYKSGLLVRED"
     gene            complement(12104..12781)
                     /locus_tag="VEW08_01755"
     CDS             complement(12104..12781)
                     /locus_tag="VEW08_01755"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HYJ39481.1"
                     /translation="MTPARRFVWKIVAAIGVFLIKTFWRSCRVVRIVGDEHMAAALAA
                     SPSLIPVYWHQHQIFCSKYLFDQKARGLKLGFLISPSVDGDIGVMVVAKFGGHSIRGS
                     TTHTGARALRDYYDALVKQGLSPSITPDGPKGPRFVFKPGAILLSQMSQRPMLPISWC
                     ASRATLFQWDKFVLPWPFSRIVIAVGPPRQVPRTMQPAALEAWQKDMAAELKRLFGVA
                     RDALAVK"
     gene            12992..14314
                     /locus_tag="VEW08_01760"
     CDS             12992..14314
                     /locus_tag="VEW08_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HYJ39482.1"
                     /translation="MSATPKDDEKPAPLPPKADVEFDIGGDFEAIHILTELAARYGDI
                     FRFHSPARRADIWVINHPDDVKRVLVSNHKNYTKGMGLDRVKILLGHGIMTSEGELWK
                     RQRYMMQPSFHRRVITEFSKLIDSSNDRFIAKWEAQAARGELVNITDDMSELTLEIVL
                     LSIFGTDLERLEKEFGSNPFDIVTRETARDLKFAYRFRQLAKLVGALAKRRREEKSEH
                     FDFLQMLMDARDKETGAPMSERELVDEAMTLIVAGHETTASGLNWCWYLLSQNPQAEA
                     QLHAEIDACAFTEPSLSDMESLSYTKSVVDEALRLYPPGWLLSRRPIAPDVLSGFEVP
                     AGTDVLFSPYLLHRHPRYWKDPASFRPERFDAAHESERPRFAYMPFAAGPRHCIGETL
                     ALFEMYMHLYKVARRYRLTYVPDRPLELEAHINLRTKYPLLMKLESRS"
     gene            14320..16308
                     /locus_tag="VEW08_01765"
     CDS             14320..16308
                     /locus_tag="VEW08_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HYJ39483.1"
                     /translation="MMTKAETLAELLVKHRGVKREISYLEAENSERVVSYEELYQRAL
                     GILFHLQKVGAKPGDKLIIYLANNEQFLDAFWAGMLGGIVPVPVALGISDEHKHKLLR
                     IAKKLGKPFIYTDRKTLDRIGAFAGPAGEQAVFDGLLSRAFFAEQVDDISKAGKVHAV
                     GPDDMAFIQFSSGSTSEPKGIVLTHRNILVNARGAGMAANWNETDTCVTWMPLTHDMG
                     LIGMHIMMYAHRMNVHIMPTDLFIRRPLLWMTFCSRKRATITSSPNFGYRHYLKVLGD
                     REPTDLDLSTVRLIHNGAEPISVELADEFMTRLAPAGLKRTAMYPVYGLAEATLVVSF
                     PQPHEQMYEFIAVDRHTLNVGKTPASLPKDHPDALTLMAVGQQVPYTQVRIAGDDDQA
                     LPDNHVGHILISGDNVTKGYFENPEANAKGFTAPDSSGVRWLRTGDLGMIQGGRLFIT
                     GRAKEIIFVNGQNYYPHDIESIAIRAEGLELGKVVAAGVRPKGASTDELILFVLHRMD
                     MQEFLPQATQVARLVNEHTGLEVAHVVPVKRIPKTTSGKIQRHLLEADFLNGVYAAEL
                     AELDSLRAAAEAAGSVKVTSTEIERRIKDICDAALERNIDVNDNLFELGASSLKLIEI
                     HEQIDRAWPGLVDLTELFDFPTIFELSRHLEGKLAATA"
     gene            complement(16275..16451)
                     /locus_tag="VEW08_01770"
     CDS             complement(16275..16451)
                     /locus_tag="VEW08_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39484.1"
                     /translation="MFAFDRSALAVLVPLALLVAVALYSNRAPDRSSAQARVQAQSEP
                     GRADQAVAANLPSR"
     gene            16570..17502
                     /locus_tag="VEW08_01775"
     CDS             16570..17502
                     /locus_tag="VEW08_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HYJ39485.1"
                     /translation="MDQLQGMRVFTRVAELGSFARAASTLDLSRAMASSYVAQLEKHL
                     GTRLLHRTTRKVSVSPQGAVYLEHCQRILAEIDAADDQLRLARNRPQGKLRVDVPVAF
                     GKYLLLPAIPAFTQRYPDISLEVRFNDHYVDIAAEHVDVAVRMGKITAPDIIARRIAA
                     SRLLTCASPRYLAQAGMPRTPEELRKHRLLGYLRSDGNRPSDWQFKQGDGTRSLRLPM
                     ALSFNTVDALTSSALDGQGLVQQVDLLVSGYISDGRLVEVLRDHSCEGPPMSVIYPKA
                     TQHLAKVRVFADFAGDLMRNYEARMRRKAEPAAR"
     gene            complement(17516..>18054)
                     /locus_tag="VEW08_01780"
     CDS             complement(17516..>18054)
                     /locus_tag="VEW08_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYJ39486.1"
                     /translation="VLHMMGNHGPAYFRRYPPEFRRFTPDCVTAELRNCSREQVVNAY
                     DNAILYTDHVLAGIVGTLAGARALDTAMIYVSDHGESLGEHGLYLHGLPYSIAPTTQT
                     HVPMIAWLSPGFSAAEGVNRRCLQARAGEPLSHDNLFHSVLGLLDVQTHVYKPERDLF
                     EGCHGHPAPAFAQNARAK"
BASE COUNT         3442 a         5777 c         5666 g         3169 t
ORIGIN      
        1 cgcatcgaca tcgcgccaca ccgacatgtt gatgatgatg ttctcgccga gcggacgcag
       61 cgaggtggcg tcgttgccgt cgcccttcag gcgccagaca aaacctggag atttttccgc
      121 gagctcgttg atgcgatcga gattgtcgac gaagtccttg agctcggggc tgtcgagtgg
      181 cgccttcagc gcggcaatgt tgagctgggc caattggtac ttcgacatcg gaactccctt
      241 ttgtttgttg cgctcagcgc atgctcttgc cgacatcgag caaagcctgg tcggcgctgt
      301 cgaccttgat gaggccgagg cgcggatatt ccagatcgac gatgacataa attgccagcg
      361 taataacgaa ggcgaacgta acgtgatgga accagctccg atccttgttg gccgacgtcc
      421 catatccgac caataacgcg ccgacgaggg agagcaaggc aaggagcacg aaaatgatga
      481 cgggcggatg attgcgcgtg gccatctggc gcgtggccgt gatgtcgatc atttcgttca
      541 atgcgggggt caggagcatg gtcgcctgcg gcgaagctgc gggcagtcgg cacgccgcca
      601 tcacctgatt ccagatctcg gcctgaagtt cagacccctt gacgatggaa gcctctacct
      661 cctcctggct cttcgcgttc ttgaagaccg aggcacggag atcgacgtag cgacggaaca
      721 ggtcacgtaa cggcgcccgg gtctcttctg gtagcaggtc cagacgcaga taggcggtgc
      781 cgatggcatt ggcttcggta ttgatgaacc agcgccgctc ctcgaaccgc gacgcggctc
      841 cggaaaacgt gaaagccagc aggagtccga gcagcgcgaa aacggctcct tcggcagcgg
      901 cagctccttt ggtgaggcct tcgggatcgt gcgcgagcct ggcaacgccg acgcggcggc
      961 cgacctcgag ggcaatcaac atgcctgcga atagagcgac tgtcagcagc atagtgatcc
     1021 cagtgaatcc cattgaactt tctcccggct acttcttttt ttgcgcgcgc tcacttgagc
     1081 ggatccttga acctgatgcc gtccggtttc gcgaacagcg ccggatcaac ctggtcccga
     1141 tgctcatagg aaaggtagcg gatcgcgtaa accacgttgt tgtccctgga gagcatgagc
     1201 gcccagggca cctggctgtc ctttgcggtg atcatcgtga cccgccaggc accgtcggtg
     1261 agctggtgct tgatcatgtc gtgtttttcg gttggcacct gctttgcctt gttggccgcg
     1321 aagaattccc actcgcagcc gaactcgagc gaagcccacg cggaaggcaa cccctctgac
     1381 ggctccagac cgagtgcagc gatagtcacg tcggatttcg gactgtcgtc gacgacgtgc
     1441 tcaccggtct tgtcgagcag attgacgacc cacatgtcgg gccaattgat gacggacaac
     1501 tgatgtatgc ccccggccgt gtccgccatt tcttcgccgc gcacgcgtcc atttccggcc
     1561 cggtaggtaa ccttcggcct gcttgcgaag ctgtctttgg gaattcccgg cgatccgttg
     1621 gcggtaacga tgcgaaacaa cccgggcggg tcgcagtcgg cgttcgccga ggccagcggc
     1681 atcagcattg cgagtgccag caggatcgtt ctcatcgaga gtctcccagc tatttcttct
     1741 ttcgggccat cgccttggcg gcattctgct tcgcgttgtg ccgcacgatg cggttgatca
     1801 gcgtcagagg caagggctgg tcggtaggaa acttcagatt cccttttggt cccgcatatt
     1861 tcgccgccgc cttctgaagc gcctcgtttc cgcgaacagg cgggtagagc ccgatgtggc
     1921 tcttgcaacc cgcgaagtac accagcatgc cttgctgacg gtacgccggc atgttgtagc
     1981 tgatgacttc agtggcattc ggcgccgcgc cacggatggc gcggcggacc ttcttcagca
     2041 gcggttgaat ttccttgggc aacgcggaga tgtagctatc aacgtctctg ggtttcatgt
     2101 cacagccctc tcgcaatcac ttctttcatg atctcgttgg tgccgccgta aatgcgctga
     2161 acgcgcgaat ccgcgaacag gcggcacacc aggtattcgt tcatgtagcc atagccgcca
     2221 tgcagctgca cgcactcatc gagcacctgc tgctgcatgt cgctgcacca ccacttggcc
     2281 atggacgcgg tttccgtgtc cagcttcccg gcgaccaggt ccacgacgca gcggtccgaa
     2341 aacacgcgcg cgatgcgcgc gatgctggcc agctccgcga gcttgaaacg cgtgttctgg
     2401 tagctccaaa ccggctggcc gaaagccttt cgctcgcgca cgtatttcag cgtctcgtcg
     2461 atgccgccct ccatggcgcc caccgcgcca acggcaatga cagtgcgttc ataaggaagg
     2521 tcatgcatga gctgatgcat cccctgccct tcggcgccgc ccagcaggtt ttcggccggc
     2581 accttcacgc cttcgaagaa cagctccgag gtgtcctggc catgcatgcc catcttgtcg
     2641 agtacgcggc cgacgcgata accgggcaga tctttcgtct ccaccatgag gatggacagc
     2701 cccttggatc gatccgcatc ggttcgcacg acgagcatga gcagcccggc gagaaacccg
     2761 ttggtgatga aggtcttgcc gccgtcgacg acgtagtggt cgccctgctt cacggcgcgc
     2821 gtgcgaatgc ctttcagatc cgaaccggtg ccgggctcgg tcatgcagat ggcgccgatg
     2881 agttcaccgg ccgccaggcg cggcaggaaa cggcgcttct gttcttccgt gccgtgattg
     2941 agaacgtaat gcgagcacat gctgtgcacg ccctgcccga acgaggcgat gccacgccgc
     3001 gcaagctcgg tgtacatgac cgcctcgtgg cggaaatcac cgcctccacc gccgtactcg
     3061 gccggtacgt ctaacaagag caagccggtg tcgccggcct tgcgccaggt ctcgcggtcc
     3121 acgtggtgct gctcgcgcca gcgcgcgtcg tgcgggacca tctcggtgtc gatgaaacgc
     3181 acgaccgtgt cgcggaacac ttcgaggtcg tcgtccatcc agggaatgat gtaggggttc
     3241 atgaacgaag gttagcgccc cgcaggcagg ctggccctct atattttcgt cagggcccga
     3301 actccacatt gcctatccaa ttgcgctggc gcctcgcgag gcgatcaccc tcgctctctc
     3361 cgtcgaacga cgccggaatg cgccggaggc gctcgaggcc gacgttatcg aatgcattga
     3421 cgctgaccac ggcatacaag attcccgcaa tccggctggt caccaccggg acgatgccgc
     3481 agtccgtgca tacatgaaac tccgcggtcc tggtcccgaa cgtgtatgga gtgacgcgtt
     3541 ccggacggcg aaccttgatc cgcagggttc cggatgggct ggacgtccag acaccaccgt
     3601 gtttcacgca aaacgaacag ccgcaggctc gcgcaggaat ctcggtcgga tccggcagcc
     3661 agtccagggt gaagcggatg ttgccgcagt gacagctgcc ggtgatcagc atggccggat
     3721 ttcagtccgc gccacaggcc acggagggcg cccgtgtgaa cgtgtattcg ccgccgcgct
     3781 tggccatgaa cttgcgcgac gccagggcac cttcggtctt cacggtcccg tcaaatcctt
     3841 tttcgaacag cgttgatcga aggaccacgg ccccggcatg tcccgtggtc aggtcgagct
     3901 gaacgggccc gcacgaatcc cactggatgt cgaccgtgat atcgccgcgt gcgcgaatgc
     3961 ctttgacact gcccttgccc caggctttcg gcaatgccgg caggatgtgg agctcgccat
     4021 tctggctctg caggagcatt tcaacgattc cggcggtggc gccgaagttt ccatcgatct
     4081 gaaatggtgg atgtgtatcc cacaggttgg gcagcgtgct gtcccggatc agaccttcga
     4141 gcaacttgtg tgcgcgatcg ccatcgcgca ggcgcgccca gaaattgacc ttccacgcac
     4201 ggctccagcc cgtgctggcg tcaccgcgcg catcgagcgt ggcgcgcgcc gcggcgagga
     4261 gttcggggtt tttcacaggg tcgatcgcat gccccggatg caacgcgaaa agttgcgaga
     4321 catggcggtg atcgtccctg gcgtcatcga ggtcggcttt ccattcctgg agctgccccc
     4381 atttgccgac gcgcagtccg cggtcgagct tcgcgagcgc atcatcgact cgcttcgcga
     4441 agaccttgtc gcccaccttc tgcgcggcgc ccgaggtgtt cacgaacagg tccgcgacga
     4501 tctgctgcga catcgcggcg cccgcggtga aggggccatg ctccggagag tagctcgggc
     4561 tcactatcag tgtgccgtcg cgcggatcgg ccaccagcgc gtccagccag aactccgctg
     4621 cgcctttcat gaccggccag gcgcgcttct tcaggaactc caggtcctgg ctgaatagat
     4681 agtgctcgta ataatgatgg gcgagccacg cggatgcttc cggctgccag aacgccgtgg
     4741 gccagtcgat tggcccaacg tatccccagg cattggtgtt caggaacatc gtccagccat
     4801 tggcgccgaa atagcgctgc gcggcgagct tgccgggtgg cacgaggtga tcgacgaaat
     4861 caaacagcgg cggcgtggtc tcggtgagat ttgcgacctc tgccggccag tagttcatct
     4921 gcaggttgat gttgacgtgg tagtcggcgt tccacggcgg cgtagccttg tcgttccaca
     4981 cgccctgcag gttcgctggc agcgaaccct cgcgcgaaga cgcaatgagc aggtagcggc
     5041 cgtactggaa atacagctgt tcgagctggc gatcgagtgc cgcattaccg gtgccgtatt
     5101 gcgcaagcag cttgtcggtc ggcagcttgg acacgggcgc ctccaggcgc agcgcgacgc
     5161 ggcggaacaa ttcctggtga tcatcccggt ggcgcgcgaa gtattgggcg tatcgatctt
     5221 tgccctggat gcctgcatcc tttgcaactg ccgccgcggg atccgcgccg gcttcgcgat
     5281 agtcggggta gcgcatgcga tagttagtgc gcgcggccat caagaaggtg atctggcact
     5341 cgctttcgat tcgcagggaa tcgccatcgg ccttgatcga gccgcattcc ggaatcacgc
     5401 gcaattcggc ggcatactgg agtccgttcg atttcagcgc gccggaaatc atgatgcgcg
     5461 tgccttccgc gcggatggtg acgctgcggt tgtcgggaac cacgaagcgc acgcgcagtt
     5521 tctgcaggct ggtgccgtac cagcgcccca ccagcacctg gtcgggatac gaaacgaagt
     5581 actcgcggcg aaagccggca tcaccctgct tgaaactcac cttggcggtc gcggtgtcga
     5641 tatcgagctc gcggcgataa cccgacacgg gcgtgccgtc ggattcgcgc tcgatgatga
     5701 agtccccgaa actctggtag tcgccgtagt tgtgcatctt gcggccaagc tgtttggcca
     5761 cgtcttcggg cttgagctgc gcgccgtcgg tgagttgctt gccgatcgag cgcatgagct
     5821 cgacttggga gtcggcgggc aagccgaagt catatccgcc ctcggagccg gggcccccgg
     5881 tccacaggga cttttcggat atctgcaggc gctcggaatc cacgccgccg aacaccatcg
     5941 cgccgatgcg gccattgcca atgggcagcg cttccttttc ccaatcggtg gcaggctggc
     6001 cgtaccagag gacgttggcg ttctgcgcct gcgcgaacga tgcgagcgcg agcaggccgg
     6061 cgacggcagt tagacaaaaa tggcgcatga gacccccttg aacggaggga tcataaatgg
     6121 tgccgggccg ggaaaccggg ataactcggc gggcaaacag acggctatgg atacgccgag
     6181 ttatcccggt ttcccggccc ggcaccgtct aagcgttgac ggccttgagg acgcgctgcg
     6241 gctgcgcgat gccgtagccc tgcgcgtaat cgacgccgag cgaggtgagc atctggatga
     6301 tctcggcgtt ctcggcaaat tcagcaatgg tcttcttgcc ggtcaagtgg ccgatttcat
     6361 tgatggaccg caccatctcg cggtcgatgg ggtcgtgcag gatttcgcgc acgaaactgc
     6421 cgtcgatctt gaggaagtcc accgggaagt gcttgaggta accgaacgac gacaggccgg
     6481 tgccgaagtc gtcgagcgcg aagcggcagc cgagttcttt cagcgcctgg atgaagcggt
     6541 tggcctgcga gaagctggcc acggccgcgg tctcggtgat ctcgaaacag atcttcgagg
     6601 catcgatgcc gctcttctgg aactgctcga tgacgaacgg caggaacttg tcgtcgccga
     6661 aactctggcc ggaaaggttg atggagcaca tggcgagttt ctcgcgctcg tcggcttcgc
     6721 tcaccagcca gcgcagcgcg ttctcgatga cccagcggtc gatggccggg gtcaggccgt
     6781 aacgctcggc cgcggcgatg aaattgtcgg gcgacaccat gcgaccggat tcgtcgcgca
     6841 tgcgcagcag gagttcgtag tgctggccgg gctcggcgcg ctgcagtggc tggatctgca
     6901 tgcgatacag ctcgaatcgg ccctcttcca gcgccgcgtt gatgcgcgcc gcccattgca
     6961 tctcgcgccg gcggcgcatg agctcgatat cgttctcggc gaagctgtgc acgcggttgc
     7021 ggccgccctc tttcgcggcg gcgcaggcgc tgtcggcggc cgagatgatg gacgccacgt
     7081 cttcgttgtc ggcggcgatg ggcaccacgc ccacggacgc gccgagacgg aacacgcgat
     7141 cttcccaggt gaatctgaag ttccggacgg cctcgcgcag cacctcggcg gtacgcaacg
     7201 cttcatcgag cgagcaggcc tcgagcagga tgccgaattc gtcaccgccc aggcgcgcga
     7261 gcgtatcgcg ccagcgcact ttgctcttga gcagcgcgcc gacctggcct aacaacacgt
     7321 cgccggcgct gtgcccgcag gtgtcgttga tgatcttgaa ctggtcgatg tcgaggtaac
     7381 acagcgcgta ggagctctcg cgcgccttgg cgctcttcaa agcccgctcg acgcggctct
     7441 cgaactcgcg ccgattcacg aggccggtca gcaagtcatg tgaggcgtga tacgacaggc
     7501 ggcgattcag ttcccgcgac tcggtgacgt catggaacac gagcacgccg ccggacaccg
     7561 cgcccgcgcc gtcccggatc ggggccgcgg tgctttccac gtacagctcg ttgccgtcgc
     7621 gacggatgag cagcatcgga cgcacgctct tgatgggccg cgtgcggcgg atcgagacgc
     7681 tcagcggatt ctcgagcggc tcgcaggttt cttcgtggaa cgccctgtag atctcttcga
     7741 tgggccgtcc catcgcatct tcgaggcgcc agccggtcag ctgttcggcc accgggttga
     7801 tgtagtcgac cgtggagttc gcgtccgtcg tgaccacgcc gtcaccgatg gattgcagcg
     7861 tgatctgcgc gctttccttc tcgcggaaca gcgcctcttc gtacagcttg cgttccgtga
     7921 tgtcgagctc gacgccaacc aggcggagca gccggccgtg ttcgtccacg cgcgccttgg
     7981 cgcggctcac cacccagcgc cagtcaccgt tgcggtgctt catgcgatgg acgctttcga
     8041 aaatcggcga cttgccggcg acgtgatcgc ggatagcgga ctgcacgcgc gacatgtctt
     8101 cagggtgcac gagactgcgc cagtcgggcg aatcgagctg cgcgtcgacc ggatagccca
     8161 gcatctgctt ccagcgcggc gagaaatgga cttcgttgct ctcgatgtcg aagtcccaaa
     8221 gaccgtcgtt ggccgcgccc tgcgcgagct ccagacgttc ttcgagtttg tgcaggcgtc
     8281 cttcgacacg taaccgctcg aggcctgcgc ccagggaatt gccgagcagc ttcaacagca
     8341 actgcaggtt tacatcccag gcgccgcgcg gcagcgcgtt cgcgagtccc aggattcccg
     8401 ccggcacgcc gccgacacgg aaagcaatca tgagcactga accgatctgc agctccgcca
     8461 ggcgctggct ctcgatgatc tgctcccggc ggggcgcgga actgtcgcga atctcggaga
     8521 gacgaaggtg atcgaggcgg cccttgagcc agggataggt atcgatggag gcgctgcgcc
     8581 aggcttccac gcggcattgc gcgagcgcgc cgtgggcgga atagacctct tcgatggtgc
     8641 cgccgtccgg agagaacagc cacacgaaag cggtatcgac ggccgaggaa tcacgcaggg
     8701 aattgaggct gtcgcggatc gtgctttcga tgccgccgcg acgcatgttc tgcaggtcga
     8761 tggatatttt ggtgcaggcg atatccacac cgagcgccgg cttgccaccg cgcgcggacc
     8821 atacgcccgt cgcaaagacg tcggacgcgg tcagatcacc aggatgttca ggtgcttgag
     8881 ccactcttta tgcaatctac agcgtagatc cgtgtgccgc gcccaaccgg gccacagatt
     8941 cgaacgcctg actcctccca aagttgcgaa gcattctgtc ataagaatcg aagaacacaa
     9001 gctaagtgca tggtgcaacg agagaatgat tcgcaattgt tcaagattcg tacagtgtta
     9061 agtctcaatg gccggtcggc gcatatgcga ttgcacgcga cgagtcgccc tgctccgcgc
     9121 aggaacgctc cgccggggcg cgcacgagga accactcctc gtctgaagtc cggccggcag
     9181 gggtcaccga cacgaggaag cacgggtact tcttcatcgt ggattcgggc accagcagta
     9241 cgcggttcgg cgccgtattg agccactgcg cagcgtcgta cgactcctgc ggaccttcga
     9301 gccagcgacg gtgaccaaag ttgatggtcg gctgatcgag atacagcagg aactgttcct
     9361 tgtatgcgac cagcgcgagg aactcgccgg gtttcaactg cgccagcact gcacgcgtga
     9421 agtcttttcc cgagcgttgg ccattcatcg ccggagcgat gagataggag aacacgatgg
     9481 cgagcgaacc aatcgccacc ggccaggccg caagcggcgc acgcagcgcg gcgaaggcca
     9541 gccccgcacc gcataacgcg aggtagacgt acagcgacgt gacgccgcca agattggctg
     9601 cggcaatggc ctcccgggca aacttcatgt ccacggtgta agcgatggcg aggaccgctg
     9661 ccgcgacgaa aaatatccca ccgagcacga atcccgcccg ccgcacaccg gcgcgcgtca
     9721 gcagcgactc gaggtatggc agcgcggcga tcgccagcgc gggcaacgcc ggcaggatgt
     9781 agatgccgcg cttgccgggg ctcgcggaga agaacgccag caccaagagc acccagaaca
     9841 gtggcagcca cacgcgcgca ttacgttcat ggaaagccgc cttgaagcgc ggcgccagcc
     9901 agatcagcag caggctccag ggcagccaca acgcggggat gacctcgaga atgaagtaat
     9961 accaggcctt gacgtgatgc caggctgccg cgtaacggcc gaccgtctgc ttgaacagga
    10021 tttcgtcgcg ataagcggcg tactcggcgg aaccgctggc ggcggcgacg agcatgggca
    10081 cgaaccacaa cgaaatcgcc atcagcatcg cgagcggcgc cagccaccag cgccaaccga
    10141 ccgcgcccgc atcgatgggc ggcaggccat tccaccgaaa cgcgcgcaac gaaaagtacg
    10201 gaatgagcaa cagcacgggg agaaatccca cgcccttggt gaacacgccg agtcccgcga
    10261 cgaatccgcc gatgaagtac cagccccacg ccggccccag cagcaggtgg cgcaacagtg
    10321 cgtacagcga gaacgtcgtg aggaaacaca acgtcgcgtc gatctgtgca ccgcgcatga
    10381 ccatgaggaa ttgcagcgtg cagacgctga tcaacgcggc agcaaggccc gcttcgcggc
    10441 tgaccgtgcg gcggccgaaa tcgtagatca ggaacagcgt gccgcccgcg gcgagaaacg
    10501 agggaatcag aaacgacgcc ttgaccgagc cgaacaacgt gtagcagatc gccagcagcc
    10561 agaagtacaa cggcggtttg tcctggtaga ggtcgccgcc cacgcgcgga aacaaccatt
    10621 catgcgagag cgccatgtca cgcgccagcg ccgcgaaccg cggctcgtcg gcgggccagg
    10681 gatcccggac gccaagcccg gtgccaatga tgattgcgag tgccagcgcc agccacagga
    10741 gatcgcgagc gcgaatgggc gcgagccccg cgggctgcgt cggtatcgac gacgcccctt
    10801 tcccgcctgg agacgcgacc ggcgccgcgg actccggggg cagcgccgcc agtccggctc
    10861 ctgcggccga cggcgcacta actactgccg tgaccacggc gatggcgctg tcgatgggcg
    10921 cgagaaacgg cgcggcgatt tcgcggtctg gcgccactgg cgcgccagcg ggcacgccct
    10981 cttcggcagc cgggactttg agcggaggaa cgacgcgcgg gcgcggggcg ccgccctgga
    11041 gaaggccgat atcaggcatt ctgacctgcg cgtttcgagc gaatgtccat cacgaggttg
    11101 tagaacgcca ccgacgccgg cagcaggttg gtcaatacac ccaccgaatc attcttgccc
    11161 cagatgaaat acgacgtgac catggcgctg cccagcagac tgatgatcca gaagctgcgc
    11221 ggaatcattg gccggccggc gcgcttcgtc gcccaggcct gcaccaccca gcggcccgcg
    11281 aacatcagcg cgccgagaaa tcccacgagc ttccacggcg tcagcacgac accgaaatag
    11341 acgccgatca cgccactcat catgggacgc tcctcacagg ttcagtcctc gcgcaccagc
    11401 aggccggact tgtaacgccg gcgcagccac atgacgccgc agatgtcgac gatgcccacc
    11461 cacagtcggt tgagcattcc gtatttcgac gtgccgcgcg tgcgttcccg gtgactcacc
    11521 ggaaccgaaa tcacgcgtgc gccgtggcgc tggaacagcg ccggcagaaa acgatgcatg
    11581 tggtcgaaac gcggcaggtc catgaagacg tcgcgcgaaa agagtttgat gccgcagccg
    11641 gtgtcgggcg tgccatcgcc taacaaaccg ccgcgcacgc cattggcgat gcgggactgc
    11701 agccggcgaa acgccgtgtc cttgcgcgag gccgtccggt tgcccatgaa cagctgcaca
    11761 ccgcggcctt cagccgagtc acgcgccgca atgagcttcg ggatgtcggc cggatcgttc
    11821 tggccatcgc cgtcgagcgt cgctacccac ggggcgcgcg ccgcgcgcac gcccgtcgcc
    11881 accgccgcgc tctgcccgct gcgaaaggag tggcgcaaca cccgcaaggc ggacagcgag
    11941 ttcttgagct tcagcagatt ggacgcggtg tcgtcggtgc tgccgtcatc gatgaaaatc
    12001 atctcgtatg aacggccggc cagcgccgcg tcgatctcgc gcgccagcgg ctcgacgttc
    12061 tccgcttcgt tgcacaccgg caccacgact gaaaattcga tgctcatttg accgccagcg
    12121 cgtcacgcgc gaccccgaac aacctcttga gctccgccgc catatccttt tgccaggcct
    12181 cgagcgccgc gggctgcatg gtgcgcggca cctggcgcgg tggtccgacg gcaatcacga
    12241 ttcgcgagaa tggccagggc agaacgaact tgtcccactg gaacaaggtg gcgcggctcg
    12301 cgcaccagga gatcggcagc atcgggcgct gtgacatctg cgacagcagg attgcgccgg
    12361 gtttgaatac gaagcgtgga cccttcgggc cgtccggcgt gatggaggga gacaggccct
    12421 gcttcaccag cgcgtcgtag tagtcacgca gcgcgcgcgc accggtatgc gtggtggaac
    12481 cgcggatgga gtgccccccg aatttcgcga ccaccatgac accgatgtca ccgtcgaccg
    12541 aaggactgat gaggaatcct aacttcaggc cgcgcgcctt ctggtcgaag agatacttgg
    12601 agcagaagat ctgatgctga tgccagtaga cgggaatcag tgacggcgat gccgccagcg
    12661 ccgccgccat gtgttcgtcg ccgacgatgc gcacgacgcg gcagctgcgc cagaaggtct
    12721 ttatcaggaa caccccgatc gcggcgacga tcttccagac gaagcgacgc gcaggagtca
    12781 tccgacgctt tgaccgggtc tccttcttat agagggtgtt gcgcagtccg cctgcgtctt
    12841 cgggatcggt tggcatccgg aggggccggg aaaaagaggc gcgaattgtc ggtgaatcgc
    12901 cttccggctg caatgaaatg cgcgcgatgg cgacggggtc gccgggacgc cgacgtctaa
    12961 cgcccgcggc cttgctatat catccgggcg catgtcggcg acccccaaag atgacgaaaa
    13021 gcctgcgcca ttgcctccca aggcggacgt tgaatttgac atcggcggcg acttcgaggc
    13081 gatccacatc cttaccgagc tcgccgcccg ttacggcgac atcttccgat tccattcgcc
    13141 ggcgcgccgc gccgatatct gggtcatcaa ccaccccgac gacgtgaagc gcgtgctggt
    13201 gtcgaatcac aagaattaca ccaaggggat ggggctggac cgggtgaaga tcctcctggg
    13261 ccacggcatc atgaccagcg agggcgagtt gtggaagcgc cagcgctaca tgatgcagcc
    13321 ctcgttccac cgccgcgtca tcaccgagtt ctcgaagctc atcgactcgt ccaacgaccg
    13381 cttcatcgcg aaatgggaag cgcaggcggc gcgcggcgaa ctcgtgaaca tcaccgacga
    13441 catgagtgag ctcacgctcg agatcgtgct gctatcgatt ttcggcacgg acctggagcg
    13501 gctggagaag gagttcggca gcaatccgtt cgacatcgtc accagggaaa cggcgcgcga
    13561 cctcaagttc gcgtaccggt ttcggcagct cgccaagctc gtgggcgcgc tcgcgaagcg
    13621 ccgccgcgag gaaaaatccg agcatttcga tttcctgcag atgctcatgg atgcgcgcga
    13681 caaggaaacc ggcgcgccca tgtccgagcg cgagctggtc gacgaggcca tgacgctcat
    13741 tgtcgcgggt cacgagacca cggcgagcgg cctcaactgg tgctggtacc tgctctcgca
    13801 gaatccgcag gccgaagcgc agctgcacgc ggaaatcgac gcctgtgcgt ttacagaacc
    13861 cagcctctcg gacatggaat cgctgtcgta caccaaaagc gtggtcgacg aggcattgcg
    13921 gctgtatccg cccggctggc tgctgtcgcg ccggcccatc gcgcccgacg tgctgtccgg
    13981 cttcgaggtg cccgccggca ccgacgtgct gttctcaccc tatctactgc accgccaccc
    14041 gcgatactgg aaggatccgg cctcgttccg gcccgagcgc ttcgatgcag cgcacgaaag
    14101 tgaacgcccg cgtttcgcct acatgccgtt tgccgcgggc ccgcgccact gcataggtga
    14161 gaccctggcc ctatttgaaa tgtacatgca cctgtataag gttgcccgcc ggtaccggct
    14221 gacgtatgtc cccgatcgcc cgctcgaact ggaagcgcac atcaacctgc gcaccaagta
    14281 cccactcctg atgaagctgg aaagcaggtc ctgaaacaca tgatgacgaa ggcagaaacc
    14341 ctggccgagt tgctcgtcaa gcatcgcggc gtgaagcggg aaatcagtta cctcgaggcc
    14401 gagaacagcg agcgcgtggt cagttatgaa gagctgtacc agcgcgccct cggcatcctg
    14461 ttccacctgc agaaggtcgg cgcgaaaccg ggtgacaagc tcatcatcta tctcgccaac
    14521 aacgaacaat tcctcgacgc cttctgggcg ggaatgctcg gcggcatcgt gcccgtgccc
    14581 gtggcgctcg gcatcagcga cgagcacaag cacaagctgc tgcgcatcgc gaagaagctg
    14641 ggcaagccgt tcatctacac cgaccgcaag acactcgatc gcatcggcgc cttcgccggc
    14701 cccgccggcg agcaggcagt gttcgacgga ctgctctccc gcgcgttctt cgccgaacag
    14761 gtggacgaca tttcaaaggc cggcaaggtg cacgccgtcg gccctgacga catggcattc
    14821 atccagtttt cctccgggtc gacgagcgag ccgaagggca tcgtgctcac gcaccggaac
    14881 atcctcgtca acgcgcgcgg cgccggcatg gcggcaaact ggaacgaaac cgacacctgc
    14941 gtgacctgga tgccgttgac ccacgacatg ggcctcatcg gcatgcacat catgatgtac
    15001 gcgcaccgca tgaacgtgca catcatgccg acggacctgt tcatccgccg cccgctgctg
    15061 tggatgacgt tctgctcgcg caagcgcgcc acgatcacca gctcgccgaa cttcggctac
    15121 cggcactatc tcaaggtcct gggcgatcgc gagcccaccg acctggacct ctccactgtg
    15181 cgcctcatcc acaatggcgc cgagccgatt tccgtggagc ttgcggatga attcatgacg
    15241 cggctcgcgc ccgcggggct caagcgcacc gccatgtatc cggtgtacgg actcgccgag
    15301 gcaaccctgg tggtgagctt cccgcaaccg cacgagcaga tgtacgaatt tatcgccgtc
    15361 gatcgccaca cgctcaatgt ggggaagacg ccggcgtcac tgcccaaaga tcatccggac
    15421 gcgttgaccc tcatggcggt gggacagcag gtcccataca cgcaggtgcg catcgctggc
    15481 gacgacgacc aggcgctgcc cgacaatcac gtcggccaca ttctcatctc gggcgacaac
    15541 gtcaccaagg gttacttcga gaatccggaa gcgaatgcca agggtttcac cgccccggat
    15601 tccagcggag ttcgctggct gcgcaccggc gaccttggaa tgatccaggg cggcaggctg
    15661 ttcatcaccg gacgcgcaaa ggaaatcatc ttcgtcaacg gccagaacta ctatccgcac
    15721 gacatcgaga gcatcgcgat ccgcgccgaa ggcctcgagc ttggcaaggt cgtggccgcg
    15781 ggtgtgcggc ccaagggcgc gagcaccgac gaactcattc tattcgtgct ccatcgcatg
    15841 gatatgcagg aatttctgcc gcaggccacg caggtggcgc ggctggtcaa tgaacacacc
    15901 ggactcgagg tggcgcacgt ggtgccggtg aagcgcatcc cgaaaaccac cagcggcaag
    15961 atccagcgtc acctgctcga agcggacttc ctgaacggcg tgtatgccgc ggagctcgcg
    16021 gaactggaca gcctgcgcgc ggcggcggag gcggccggca gcgtgaaggt caccagcacc
    16081 gaaatcgaac ggcgcatcaa ggacatctgc gatgcggcgc tggaacgcaa tatcgacgtc
    16141 aacgacaacc tcttcgagct cggcgcgagt tcgttgaagc tcatcgaaat ccacgagcag
    16201 atcgaccgcg cctggccggg gctggtggac ctcaccgagc tcttcgattt cccgacgatc
    16261 ttcgagctgt cacgtcatct cgaaggcaaa ttggcagcga cggcctgatc ggcgcgcccg
    16321 ggttcggact gagcctggac ccgggcctgg gctgatgatc gatcgggcgc gcggttggaa
    16381 tacagtgcca cggccaccag cagcgccagc ggcacgagga cggcaagagc ggaacggtcg
    16441 aaggcgaaca tgggttttgg ctccatcagg ttgggttaaa acttacccga cgcagcccgc
    16501 tcgaaaaacc ggacaaaacc gaattcattg tctggtcctg cttgacaatc atcgctagaa
    16561 tgtcgcgcca tggaccagct tcagggcatg cgcgtgttca cccgggtggc ggagctgggc
    16621 tcttttgccc gcgccgccag cacgctcgac ctgtcccgtg ccatggccag cagctacgtg
    16681 gcccagctcg agaagcatct gggcacccgc ctgctccacc gcaccacgcg caaggtctcg
    16741 gtctcgccgc agggcgccgt gtacctggag cactgccagc gcatcctcgc ggagatcgac
    16801 gcggctgatg accagctgcg cctggcgcgc aaccggccgc agggcaagct gcgggtcgac
    16861 gtgccggtgg ccttcggaaa atatctgctg ctgccggcga tcccggcgtt cacgcagcgc
    16921 taccccgaca tctcgctcga ggtgcgcttc aacgaccact acgtggacat cgccgccgag
    16981 cacgtcgacg tcgcggtgcg catgggcaag atcaccgcac cggacatcat cgccaggcgc
    17041 atcgcggcct cacggctgct cacctgcgca tcgcccaggt atctggcgca ggcaggcatg
    17101 ccgcgcacgc ccgaagagct gcgcaagcac cggctcctcg gctacctgcg cagcgacggc
    17161 aaccggccct cggactggca gttcaagcaa ggcgacggca cgcggtctct gcgcttgccc
    17221 atggcgctct cgttcaacac ggtcgatgcg ctgacgtcgt ccgcgctcga cggacaaggc
    17281 ctcgtccagc aagtcgacct gctggtgagc ggctacatct ccgacggacg tctggtcgaa
    17341 gtcctgcgcg accactcctg cgaaggcccg ccaatgagcg tgatctatcc gaaagccacg
    17401 caacatctgg caaaggtccg ggtgttcgcc gatttcgccg gcgacctcat gcgcaactac
    17461 gaagcgcgca tgcgacgcaa ggccgaaccc gccgcgcgct gagcgcgccg gctttctact
    17521 tcgcgcgtgc gttctgcgcg aatgccggtg cgggatggcc gtgacatcct tcgaacagat
    17581 cccgttcggg tttgtagaca tgggtctgca cgtcgagcag cccgagcacg gagtgaaaca
    17641 gattgtcgtg tgacaacggc tccccggccc ttgcttggag gcagcgccgg ttgacgcctt
    17701 ccgcggcgga aaatccaggc gaaagccagg caatcatcgg cacgtgcgtc tgcgtagtcg
    17761 gcgcgatcga atacggcagt ccatgcaggt acaaaccatg ctcgccgagc gactcgccat
    17821 gatcggacac gtagatcatg gccgtgtcga gcgcccgggc gccggcgagc gtgccaacga
    17881 tgcccgccag cacgtggtcg gtgtagagaa tcgcgttgtc ataggcgttg accacctgct
    17941 cgcgcgaaca gttgcgcagc tccgcggtca cgcaatcggg cgtaaaacgc cggaactcgg
    18001 gtggatagcg ccggaaatac gccggcccgt gattgcccat catgtgcagc acga
//
LOCUS       DASQJS010000044         6214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1281395, whole genome shotgun sequence.
ACCESSION   DASQJS010000044 DASQJS010000000
VERSION     DASQJS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281395"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VEW08_01785"
     CDS             complement(<1..788)
                     /locus_tag="VEW08_01785"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HYJ39487.1"
                     /translation="MKIRRAAVGTSIAALLPFAGTALAQNAQPSDSNAELRREIEDQK
                     QRLLVLERKLEIQQEAATAAAATAPRITASASRFQIGSTDNSNFIRLRGTLHADARTY
                     GGDSVPEIADTFILRRVRPTIEGTFGGIYDFKFMPDFAGGRSVIVDAYTAARFKPGAI
                     VTVGKFKPPVGLERLQSSADIRFIERALPTNLVPNRDLGVQLSGDFSGGAISYAVGYF
                     NGVTDGQSSDNLATPDIEVDTAGDYAARLFFQPFANSSNFNLRGL"
     gene            complement(927..1211)
                     /locus_tag="VEW08_01790"
     CDS             complement(927..1211)
                     /locus_tag="VEW08_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39488.1"
                     /translation="MSTHNHIPRTVEACLDARLAPFHGIEPRSALEDLDVEVSRAVSP
                     RQERLQRLSLLNDLKLLFAALGAGGSVVPLGDNGRTAHLLAQYAAALREH"
     gene            1312..2268
                     /locus_tag="VEW08_01795"
     CDS             1312..2268
                     /locus_tag="VEW08_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HYJ39489.1"
                     /translation="MTAAYARLPLNALRVFEAVASRLSFADAAEALHVTPAAVSQQVK
                     SLEDYLQVQLFKRAGRRIELTAEGQQLLPGVRRGLDELEASMQHLKQHRAEGPLQITL
                     LSSFLQRWLLPRIGSFNDLDTGVELRFHTGRDPVDFSRSEMHVAIRMGLGRWPGLHVE
                     KLLDEWVIPIGSPGLLAKYGPVPRGEDLQNFPLLGSMDEPWQAWIETGQEGNWLARAP
                     IIDDSAGVIAAAEEGIGFALARWSLVQRSLEQGRVVLAGDALLPFRFSYFFVCPEPYL
                     VMPKVKRFRDWIFDMAKSFPKPHDLFTRSVARSAAKSIPRKG"
     gene            complement(2485..3585)
                     /gene="modC"
                     /locus_tag="VEW08_01800"
     CDS             complement(2485..3585)
                     /gene="modC"
                     /locus_tag="VEW08_01800"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HYJ39490.1"
                     /translation="MLRVELQKRRGEFTLDAAFHAPTPGVTALFGRSGCGKSTLISLI
                     AGLLLPDSGRVVLGDDVLFDGERHLSVDARHRRIGVVFQDARLFPHLSVRGNLDYGAR
                     RVARDSPRPVAFDDVISLLGLAGLLGRRPHELSGGEKQRVAFGRALLSQPRLLLLDEP
                     LASLDLARREEVLPYLERLRDAFAIPIVYVSHQFDEVLRLATRVVLIEAGRTVADGDI
                     AEVSRHPQLRAIVGPDSVGAVLAGHVERLDATGLAVLRVGDAELSVELEGAVVNQRIQ
                     IQVLARDVIVASERPVGLSVRNVVVARVISVTPDAGRAVLVELDIGRTATLLARITAR
                     ASEELGLSTDKQVWALIKAASLRGHVFSVPAR"
     gene            complement(3585..4271)
                     /gene="modB"
                     /locus_tag="VEW08_01805"
     CDS             complement(3585..4271)
                     /gene="modB"
                     /locus_tag="VEW08_01805"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006939.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HYJ39491.1"
                     /translation="MLSPAESEALWLSLSVAARSVAFNLPVAIVVAWLLTRTRFAGRT
                     LFDAFVHLPLVLPPVVIGYLLLVVFGVRGPVGGWLHENFGIQLAFTSTGAALATAVMS
                     FPLVVRAIRISLENVDRGLEEAARTLGAGPFDRFFSITLPLITPGILAGVVTAFAAGL
                     GEFGAVITFAANIPGETRTLPLALYTALQTPDGDALAARLALISLSLGLAGLLVSELL
                     SRALRRRLGR"
     gene            complement(4280..6046)
                     /gene="ybaL"
                     /locus_tag="VEW08_01810"
     CDS             complement(4280..6046)
                     /gene="ybaL"
                     /locus_tag="VEW08_01810"
                     /inference="COORDINATES: protein motif:HMM:NF007950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HYJ39492.1"
                     /translation="MPAELHETSLIALLAVGFVLALLFGLVAGTLKVSPIVGYLIAGI
                     CVGPFTPGFVADAGLANQLAEIGVILLMFGVGLHFSIKDLMSVRYIALPGAVAQIVTA
                     TAIGAGMAHLLGWSFGTGLVFGLSLSVASSVVLLRALEERNALQSSEGKIAVGWLIVE
                     DLAMVLALVMLPALAETLGGDASKVNAHTGSQNTWLALAITLAKVAGFVALALVVGTR
                     VVPWLLKRVSRTASRELFTLCVLAVALGIAYSSSALFGVSFALGAFFAGVILSESELS
                     HQAGNDLLPLKDAFAVLFFVSVGMLFDPTVLVREPLAVLSVLAIIMVGKSLAAFIIVL
                     AFRHPVRTALTVSASLAQIGEFSFILVGLGAALGLMPPDGRDLILAGALLSIALNPVA
                     FAGISPLTRWIEARPKLLQTLERRGADRHRNPVSSTAGLLNHAILVGYGRVGGAIGPI
                     LKEQGLAFAVIERDHLMLSTAQSQGIPTIEGDAAAPGVLTAAGVDTARLLVVATPDSF
                     LARRIVEVARKLNPTIDVVVRTHSESEFKALEALGAGRVVMGERELARGMLEYALRSL
                     GVPRERAQALAGRDAQVNPEGT"
BASE COUNT         1150 a         2004 c         2070 g          990 t
ORIGIN      
        1 aggccgcgca gattgaagtt gctggagttc gcgaacggct ggaagaacag gcgcgcggcg
       61 taatcgccgg cggtgtccac ttcgatgtcg ggcgtggcga ggttgtcgct gctctgcccg
      121 tcggtcacgc cgttgaagta acccacggcg taactgattg cgccgccgct gaagtcaccc
      181 gaaagctgca caccgaggtc gcgattcggg accaggttgg tcggcagcgc gcgctcgatg
      241 aagcggatgt cggccgagct ttgcaggcgc tcgagcccca cgggcggctt gaacttgccg
      301 acagtgacga tggcaccagg cttgaatcgt gcggcggtgt atgcgtcgac gattaccgac
      361 cggccgccgg cgaagtccgg catgaacttg aagtcgtaga ttccgccgaa ggtgccttcg
      421 atcgtcggcc gtacgcgccg caggatgaag gtgtcggcga tttcgggaac cgagtcgccg
      481 ccgtaggtgc gtgcgtcggc gtgcaacgtg ccgcgcagac gaatgaagtt cgagttgtcg
      541 gtcgaaccga tttggaagcg tgatgcgctc gccgtgatcc ggggcgcggt cgccgcagcg
      601 gccgtggccg cttcctgctg gatctcgagt ttacgttcga gcaccagcag ccgttgcttc
      661 tggtcctcga tctcgcgacg caactccgcg ttgctgtcac tcggctgggc attctgcgcc
      721 agcgccgtcc ccgcaaatgg cagcagtgcc gcgatggacg tacccactgc tgctcgtctg
      781 attttcacct tcgttctcct ggattcgtgt ttgccacaag gccaggagag cgtaggaatt
      841 caggggttat gccgtcagtc gtgacccggt aaccctgcgg ttacggctca agtccatttg
      901 gatatagcca tatgaaaagc cggctctcaa tgctcgcgca gcgccgccgc gtactgggca
      961 aggagatgcg ccgtgcggcc gttgtcaccg agcggcacga cgctcccacc cgcgcccagc
     1021 gcggcaaaca gtagtttgag gtcgttcagc aatgacaggc gctggaggcg ttcctggcgc
     1081 ggcgacacgg cccgggagac ctcgacgtcg aggtcttcga gggccgaccg cggctcgatg
     1141 ccatgaaacg gcgcgaggcg cgcgtcaagg caggcttcga cggttcgggg tatgtggttg
     1201 tgagtgctca tgggggcggt ctccggtgct gaacttgcga taggtacaat ctgcgctaga
     1261 gtcccgaacc gaacaaacgc gccttctgaa gcgcgcataa ggaaaactga cgtgactgcc
     1321 gcctatgccc gcttgccgct caatgccctg cgcgttttcg aggccgtggc atcgcgcctg
     1381 tcgtttgcgg acgccgccga ggcgctgcac gtgacccccg cggcagtaag tcagcaggtc
     1441 aagtcgctcg aggactacct gcaggtacag ctgttcaagc gcgccggccg gcgcatcgaa
     1501 ctcacggccg agggacagca gcttttgccc ggcgtccggc gcgggctcga cgagctcgag
     1561 gcctcgatgc agcacctgaa acagcatcgc gccgaaggtc ccctgcagat caccctgctg
     1621 tcatctttct tgcagcggtg gctgttgccg cgcatcggca gcttcaacga tctcgatacc
     1681 ggcgtggaac tgcgtttcca caccggccgc gatccggtcg atttttcgcg ctccgaaatg
     1741 cacgtcgcca tccgcatggg cttgggcaga tggcccggcc tgcacgtgga gaagctgctc
     1801 gacgaatggg tgatcccgat cggctcgccc ggattgctcg cgaaatacgg gccggtgcct
     1861 cgcggcgagg acctgcagaa cttcccgctg ctcggcagca tggatgaacc ctggcaggca
     1921 tggatcgaga caggccagga aggaaattgg ctggcacgcg cgcccatcat cgatgattca
     1981 gcgggcgtga tcgcggccgc tgaagagggc atcggttttg cgcttgcgcg ctggtcgctg
     2041 gtgcagcgtt cgctcgaaca gggacgcgtg gtgctcgccg gcgacgcact gctgccgttc
     2101 cggttctcgt atttcttcgt ctgccccgag ccctatctgg tgatgcccaa ggtcaagcgc
     2161 ttccgcgact ggatcttcga catggccaag agctttccga agccgcacga cctgttcacg
     2221 cgcagtgtcg ccaggagcgc cgcgaaaagc atccccagga aggggtaggc gcgaagtcga
     2281 tctctttagt tttgttgatg ccgcattcag gcatcgcgtt tgttagccgc gtgggccgcg
     2341 cgcacgatgc actcctgttc tcaaccaatc aaacaggagt agaaatcatg ggaatcgaga
     2401 aactggttcg caacctgggc tttgccctcg cagccgtcgt cgtcgtgctc ggcctgggcg
     2461 cgaccttcga atatcagccc gtcatcaacg cgctggcacg ctgaacacgt gcccgcgcaa
     2521 ggaggccgcc tttatgagcg cccacacctg cttgtccgtc gatagcccca gttcttctga
     2581 cgcacgcgcc gtgatgcgag ccagcaaagt cgccgtgcgg ccgatgtcga gctcgacgag
     2641 cacggcgcgc cccgcatcgg gtgtcaccga gattacgcgc gccaccacga cattgcgcac
     2701 cgagagtccc accgggcgtt cggacgcgac gatgacgtcg cgcgccaata cctggatctg
     2761 gattcgctga ttcaccacgg cgccttcgag ctcgacgctg agctcggcgt cgcccacgcg
     2821 caggaccgca agcccggtcg catcgagacg ctcgacgtgt ccggcgagca ccgcgcccac
     2881 cgagtcgggt cccacgatgg cgcgaagctg cgggtggcgg ctcacttcgg cgatatcgcc
     2941 gtcggccacc gtgcgaccgg cttcgatcag caccacccgc gtcgccagcc gcagcacctc
     3001 gtcgaactgg tggctcacgt agacaatggg aatggcgaac gcgtcgcgca gcctttcgag
     3061 atagggcagc acttcctcgc gacgcgccag gtcgagcgag gccagcggtt catcgagcag
     3121 cagcaggcga ggctgcgaga gcagtgcacg tccaaacgcc acgcgctgct tttcgccacc
     3181 ggacagctcg tgcgggcggc ggccgagcag gccggccagt cctaacaagc ttatgacgtc
     3241 gtcgaaggcc acgggccgag gcgagtcgcg ggccacgcgg cgcgcgccgt agtccaggtt
     3301 gccgcgcact gacaagtgcg ggaaaagcct cgcgtcctgg aagaccacgc cgatgcgtcg
     3361 atggcgcgca tcgacagaca ggtgacgctc accgtcaaac agcacatcgt cgccgaggac
     3421 gacccgtccc gagtcgggca gcaggagccc ggcgataaga gatatcagcg tggacttgcc
     3481 gcatcccgat cggccgaata gcgcggtgac gccgggtgtc ggcgcgtgaa aggccgcatc
     3541 gagcgtgaac tcgccgcgcc gcttctggag ctcgacgcgc agcatcagcg tcccagcctg
     3601 cggcgcaatg cgcggctcag gagctcggag accaggaggc cggcgaggcc aagcgacagc
     3661 gagatgaggg cgaggcgcgc ggcgagcgcg tcgccgtccg gcgtctgcag cgcggtgtac
     3721 agcgcgagcg gcagcgtacg ggtctcgccg gggatgttgg ccgcgaacgt gatcacggcg
     3781 ccgaattcac cgaggcccgc cgcaaatgcc gtgacgacac cggcgaggat gccgggggtg
     3841 atcagcggca gtgtgatgga gaagaaccgg tcgaacgggc ccgcgccgag cgtacgcgcg
     3901 gcttcctcga gcccgcggtc gacgttttcg agcgagatgc gaatggcgcg caccaccagc
     3961 gggaaggaca tgaccgcggt ggccagggcg gcgccggtgc tcgtgaacgc gagctggata
     4021 ccgaagttct cgtgcaacca gccgccgacg gggccgcgca cgccgaacac caccagcagt
     4081 agatagccga tgaccaccgg cggcaggacc agcggcaaat gcacgaaggc gtcgaacagg
     4141 gtgcggccgg cgaagcgtgt gcgcgtgagc agccaggcga ccacgatcgc gacaggcagg
     4201 ttgaatgcga cgctgcgggc cgcgaccgac aggctgagcc agagcgcttc ggattccgcg
     4261 ggtgacagca tcgatatttc tacgttcctt cgggattcac ttgcgcgtcg cggcccgcca
     4321 gcgcctgcgc tcgttcacgc ggcaccccga ggctgcgcag cgcgtactcg agcatgccgc
     4381 gcgcgagctc gcgttcgccc atcaccacgc gcccggcgcc gagcgcctcc agcgccttga
     4441 attcgctttc cgagtgcgtg cgcaccacga cgtcaatggt gggattcaac ttgcgtgcga
     4501 cctcgacgat gcgtcgcgcg aggaagctgt cgggcgtggc caccacgagc aggcgcgcgg
     4561 tatcgacgcc ggcggcggtc aggacgccgg gcgcagcggc atcgccttcg atggtcggga
     4621 ttccctgcga ttgcgcagtg ctcaacatga ggtggtcacg ttcgatgacg gcgaaggcca
     4681 gcccctgctc cttgagaatg ggcccgatgg cgccgccgac gcgcccgtag ccgaccagga
     4741 tcgcgtggtt gagcaaaccc gcggtgctgc tgacgggatt gcgatgccgg tccgcgccac
     4801 gccgctccag ggtttgcagt agtttgggac gcgcctcgat ccagcgcgtg agcggcgaaa
     4861 tgcccgcgaa ggcgaccggg ttgagcgcga tcgacagaag ggcgccggcc aatatcaggt
     4921 cgcgcccgtc gggtggcatc aggccgagtg ccgcaccaag gcccacgaga atgaaagaga
     4981 actcgccgat ttgcgccagg ctggcggata ccgtcagcgc ggtacgcacc ggatggcgga
     5041 aggcgagcac gatgatgaag gcggccagcg acttgccgac catgatgatg gccagcaccg
     5101 aaaggactgc gaggggttcg cgcaccagca cggtcggatc gaacaacatc cccaccgaca
     5161 cgaagaacaa caccgcgaag gcgtccttga gcggaagcag gtcattgccg gcctggtggg
     5221 agagttccga ctcggacagg atcacgccgg cgaaaaacgc accgagtgcg aatgacacgc
     5281 cgaacagcgc cgatgagctg taggcgatcc ccagggcgac ggcgagcacg cacagcgtga
     5341 acaattcgcg cgatgcggtg cgcgagactc gcttgagcag ccagggcacc acgcgcgtgc
     5401 cgaccacgag tgcgagcgcg acgaagcccg caaccttggc cagcgtaatc gccagcgcaa
     5461 gccaggtgtt ctgcgatccg gtatgcgcgt tgaccttgct ggcgtcgccg cccagcgttt
     5521 ccgccagcgc gggcagcatc accagcgcga gcaccatcgc caggtcttcg acgatcagcc
     5581 agccgacggc gatcttgcct tcggacgatt gcagcgcatt gcgctcctcg agcgcacgca
     5641 gcagcaccac ggagctcgcg accgacaggg acaacccgaa cacgaggccc gtgccgaaac
     5701 tccagccgag caggtgcgcc atgccggcgc cgatggcagt ggcggtgacg atctgtgcga
     5761 ccgcacccgg cagcgcgatg taccgcaccg acatcaggtc cttgatcgaa aagtgcagcc
     5821 cgacgccgaa catcagcaat atcacgccga tttccgcgag ctggtttgcg agacctgcgt
     5881 ccgcgacgaa gcctggcgtg aacggcccga cgcagatgcc cgcaatgaga tatccgacga
     5941 tcggtgacac cttgagcgtg cccgccacca atccgaacaa caatgcaagc acgaagccga
     6001 ccgccaatag agcgatgagc gaagtttcgt gtaattccgc gggcatacgc gcgagggtac
     6061 ccgaaaaacc acgaaatgcg gccgtgcaaa gcctagaatg cgcgcctcgt ttatgaaggc
     6121 cagccaagct ctcggacccg cgttcatcgg cgccatcggc gtcgtgttcg gcgacatcgg
     6181 taccagtccc ctctacgcgt tcaaggaatc cctc
//
LOCUS       DASQJS010000045         8773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_338789, whole genome shotgun sequence.
ACCESSION   DASQJS010000045 DASQJS010000000
VERSION     DASQJS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338789"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /gene="moaA"
                     /locus_tag="VEW08_01815"
     CDS             <1..991
                     /gene="moaA"
                     /locus_tag="VEW08_01815"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HYJ39493.1"
                     /translation="RPIHDLRISVMDRCNFRCPYCMPRETFHESYRFLKSNERLDFAE
                     LLRLARVFVRAGVKKLRITGGEPLLRPNLADLIGDLTSLPGIEDIALTTNGMLLAKYA
                     TELKAAGLKRITVSLDTLDPEIFTQMSGGFGGVAEVLDGIEHARRAGLAPIKVNTVVQ
                     RGVNDHTLLDLLSYFRGTGVVVRFIEYMDVGTRNHWNPALVVPSRELAARISARWPLR
                     ALAPEYRGEVAERHAFEDGLGEVGFISSVSQPFCGDCSRARISSDGSFYTCLFATQGV
                     DLRGPMRAGATDDELFELIRGIWSGRTDRYSELRASMRDAAGLHKVEMNYIGG"
     gene            981..1484
                     /gene="moaC"
                     /locus_tag="VEW08_01820"
     CDS             981..1484
                     /gene="moaC"
                     /locus_tag="VEW08_01820"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HYJ39494.1"
                     /translation="MAANRKLTHLDAANRPVMVDVGAKTATSREASAEAMVKLPPAVA
                     RELARTGHRTKKGPVFDTAIIAGVMAAKRTHEIIPFCHPLALERCSVDIASGTNGRIR
                     IICNVAVHHKTGVEMEALMGATAAALTIYDMCKALSHDIEIGPVRLIRKTGGKRDFQR
                     LKPRKKS"
     gene            1481..2086
                     /locus_tag="VEW08_01825"
     CDS             1481..2086
                     /locus_tag="VEW08_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HYJ39495.1"
                     /translation="MNVPRIHGLVLAGGRSSRMRRDKAALEFRSGETQLDATMKLLEG
                     RVAKAFVSVRADQRADPARSRYPHIVDRGDIEGPIAGISAAFSIYPDMAWLVLACDLP
                     FLDAGTLDTLLLARDPNFDATAFVSSHDGLPEPLCAIYEPGARPALLAQIAAGRNCPR
                     KFLINAHTQLLAQPNPRALDNVNTVEEYESAMTAFHESAAK"
     gene            2093..2341
                     /locus_tag="VEW08_01830"
     CDS             2093..2341
                     /locus_tag="VEW08_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HYJ39496.1"
                     /translation="MPDRTIRVQYYALLREQAGRSDETLTTSARDARELYMELSQRYA
                     FTLPAEMLRVAINAEFGEWSQPLEAGDAVVFIPPVAGG"
     gene            2338..3951
                     /locus_tag="VEW08_01835"
     CDS             2338..3951
                     /locus_tag="VEW08_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HYJ39497.1"
                     /translation="MNAFRFSTAPLDTASLQREMRDVTCGGFAAFEGWVRNHNEGLAV
                     TRLEYEAFAELAEKEGARIVLEAIARFGVTRAGCVHRIGSLALGEVAVWVGVSAAHRD
                     EAFRACRYIIDEVKHRVPIWKKEHYENGDSGWVNCERCAAPAAHHEEYAAPAAHERDY
                     AAPVNYLHEHAHAHDHDHDHEIPSVAAPDYSRQIALREVGPEGQARLRAASVLVVGAG
                     GLGVPVLQYLAGAGVGRIGIVDADRLEPSNLHRQTWYALADCGLEKSTLAAERVRALN
                     PEVRVEPHALRLDANNADRLAAGHDLILDCSDNFATKFLLNDLALRTRKPVLFASVYQ
                     YEGQLQVVRGDEATACLRCVWPEATRDGLVGNCAEAGVLGPVPGVFGSLQALEALKLL
                     LGLPGLEPGEMLIFDLVSLNTQRLRARRTPDCDAHRRAGQSAATLSPLELEVQFGSLA
                     EAQAAGFTFVDVRDSRERADDPLPSLALCLPMSKLLTDAAMLDTGGRYLLICATGKRS
                     AAAADLLRSQGFRECRSLRGGIKGLKASA"
     gene            3948..4880
                     /locus_tag="VEW08_01840"
     CDS             3948..4880
                     /locus_tag="VEW08_01840"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ39498.1"
                     /translation="MKSTLTFVVACLLALPAAGARAEVESADHPLLDLARELQKKRKP
                     DLRVVPGAPGLRQAEHALAQAKAPADDKCAGGIGALRFAELHADLALARTGQGDFAGA
                     ASAYRSAHACRPRDPQILAALSGVLFDARDYVAAREAIDAALAIAPRAVNANRLAGNI
                     DFVEERWANATGRFRYVASSDPDRVQAGYGQLMFWLSQMRAGVPKPELVERTPGEGWP
                     QPLLLYMRGQYTEAELTVPVRRGDDEDNIQPNSSTDERLCEALFYVGEAYWARGQLDV
                     ARDYFAALVNIKVIYFLEHGLALAEIAKLRELTR"
     gene            complement(5101..6342)
                     /locus_tag="VEW08_01845"
     CDS             complement(5101..6342)
                     /locus_tag="VEW08_01845"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005193613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HYJ39499.1"
                     /translation="MDRLGRDAAAAAAVLALASTATKNRALSQIAVSLRARKAELLAA
                     NARDMEAARAKGLSGAMLDRLALDAGRIEAMAQGVEAIAALADPIGATIAQWTRPNGL
                     DIARVRVPLGVIGIIYESRPNVTCDAGSLCLKSGNAAILRGGSESSHSSRAIHACLVD
                     GLSAAGLPAASIQLVPTTDRAAVGYLLSSMTDYIDVVVPRGGKSLVARVQQEARVPVI
                     GHLEGVCHVYVDREADLAMARAIVTNAKLRRTGVCGSAETLLVDAACAATHLAPVVKD
                     LLDAGCEVRGDATVQKVDARVKPSVEQDWYTEYLDAIIAARVVDGVDAAIEHIGKYGS
                     AHTESIITANAVTAQRFLDRVDSAIVLHNASTQFADGGEFGMGAEIGISTDRFHARGP
                     VGVEQLTSYKYVVRGTGQIRP"
     gene            complement(6409..7551)
                     /gene="proB"
                     /locus_tag="VEW08_01850"
     CDS             complement(6409..7551)
                     /gene="proB"
                     /locus_tag="VEW08_01850"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HYJ39500.1"
                     /translation="MTTASPLTRARRIVVKVGSALLVDQSSGQVNRPWLESLVEDLLR
                     LRARKQEVVLVSSGAIALGRRELKLAPGALKLELSQAAAAVGQIALAHIYKELFARSG
                     VTVAQILLTLEDSEVRRRYLNARATLSELLELGALPVINENDTVATTEIRYGDNDRLA
                     ARVAQMTSADCLLLLSDVAGLYTADPNLDPTARLIPEVRDITPEIEAMGGGSASAVGS
                     GGMATKIAAAKIATSAGCAMAISLGGPLHPVRRLEEGADCSWFLPASNPENARKQWIA
                     GSLRPSGAITIDAGALQALRSGKSLLPAGVTAVSGRFKRGDTVSVLDADGAEIARGMI
                     AYSDRDASRIIGKRSADIEALLGFRGRAEMIHRDDLVLLRPDLASA"
     gene            7639..7953
                     /locus_tag="VEW08_01855"
     CDS             7639..7953
                     /locus_tag="VEW08_01855"
                     /inference="COORDINATES: protein motif:HMM:NF042799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6763 family protein"
                     /protein_id="HYJ39501.1"
                     /translation="MSREYAPTPGKWYENKDEEETFRVLSVDEDDELVEIEYEDGEIE
                     ELDLDTWHELDLELTDVPEGWSDSAVDDDDDEDEDFDEDEDDDDDDDDDDDDDDDEEE
                     SY"
     gene            7959..>8773
                     /locus_tag="VEW08_01860"
     CDS             7959..>8773
                     /locus_tag="VEW08_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HYJ39502.1"
                     /translation="MVTGAAESWYHEKESAWLYGVVAAAEQDPVKRALFVKLAAAAEA
                     QAACWLATRPELKPVFSPALRARIVARLVRQIGPRRLRGVLAAMKLRGLSVYSGSHAP
                     APHPMPTTVAEIGKRHSSAGGNLRAAVFGASDGLVSNAALVMGVAAASSDLHSVQIAG
                     AAGLLAGALSMAAGEYVSVRSQREFYEHQMDLERKELAEYPEAEAEELALIYNARGID
                     MDEARRISAILLSRPEQALDVLAREELGLSPDNLGSPFAAAWSSFLSFASGAT"
BASE COUNT         1526 a         2917 c         2866 g         1464 t
ORIGIN      
        1 ccggcccatc catgacctgc gcatctcggt catggaccgc tgcaatttcc gttgcccgta
       61 ttgcatgccg cgcgagacct ttcacgagtc gtaccggttt ctcaaatcga acgagcggct
      121 cgacttcgcc gagctgctgc gcctcgcgcg cgtcttcgtg cgcgccggcg tgaagaaact
      181 gcgcatcacc ggcggcgagc cgctgttgcg cccgaacctc gcggacctca tcggcgatct
      241 cacctccctg cccggcatcg aggacattgc cctcaccacc aacggcatgc tgctcgccaa
      301 gtacgccacc gagctcaagg cggcgggact gaaacgaatc accgtcagcc tcgacacgtt
      361 ggacccggaa atcttcacgc agatgagcgg cggcttcggc ggcgtggcag aagtgctcga
      421 tggcatcgaa cacgcccggc gcgcggggct cgcgcccatc aaggtgaaca cggtcgtgca
      481 gcgcggcgtc aacgatcaca cgctgctcga tctgttgtcc tacttccgcg gtaccggcgt
      541 cgtcgtgcgc ttcatcgaat acatggacgt gggcactcgc aatcactgga accccgcact
      601 ggtggtgcca tcgcgcgaac tcgcggcgcg catcagcgcg cgctggccgt tgcgtgcgct
      661 ggcacccgag taccgcggcg aggttgccga acgacatgcg ttcgaagacg gcctgggcga
      721 agtgggcttc atttcctcgg tgtcgcagcc gttctgcggc gactgctcgc gcgcgcgcat
      781 ctcttccgac ggctcgttct acacctgcct gttcgccacc cagggcgtcg acctgcgcgg
      841 ccccatgcgc gccggcgcca cggacgatga gctgttcgag ctcatccgcg gtatctggag
      901 cggccgcacc gaccgctaca gcgagttgcg cgcctcgatg cgcgacgccg cgggccttca
      961 caaagtcgag atgaactaca ttggcggcta atcgaaagtt gacccacttg gacgccgcga
     1021 atcgccccgt catggtcgac gtcggcgcca agaccgcgac ctcgcgcgag gccagcgccg
     1081 aggcgatggt caaactaccg ccggcagtag cacgcgaact ggcgcgcacc ggccatcgca
     1141 cgaagaaagg cccggtgttc gacaccgcca tcatcgccgg tgtcatggcg gcgaagcgca
     1201 ctcacgaaat cattccgttc tgtcatccgc tggcgctgga acgctgctcg gtggatattg
     1261 ccagcggcac caacggccgc atccgcatca tctgcaacgt tgccgtgcat cacaagaccg
     1321 gcgtcgaaat ggaagcgctc atgggcgcca ccgccgccgc actcacgatc tacgacatgt
     1381 gcaaggcgct gtcacatgac atcgaaatcg gtccggtcag gctgatccgc aagaccggcg
     1441 gcaaacggga ctttcaacgc ctcaaaccac ggaagaaatc atgaacgtcc cacgtatcca
     1501 cggactggtg ctcgccggcg ggcgcagttc gcgcatgcgg cgcgacaaag ccgccctcga
     1561 attccgctcc ggcgaaacgc agctcgatgc cacgatgaag ttgctggaag gacgcgtggc
     1621 gaaggccttc gtctcggtgc gcgcggacca gcgcgccgac cccgcgcgtt cgcgttaccc
     1681 gcacatcgtg gaccgcggcg acatcgaagg tcccatcgcc ggcatcagcg cggcgttctc
     1741 gatctacccg gacatggcct ggctggtact ggcctgtgac ttgccgttcc tggatgccgg
     1801 gacgctggac accttgctgc ttgcgcgcga tccgaacttc gatgccaccg cattcgtctc
     1861 cagccatgac ggcctgccgg aaccgctgtg cgccatctac gaaccgggcg cccggcctgc
     1921 gctcctcgcg cagatcgccg ccggccgcaa ctgcccgcgg aaatttctca tcaacgccca
     1981 tactcagctg ctcgcgcagc ccaatccgcg cgcactcgac aacgtgaaca ccgtggaaga
     2041 gtatgaaagc gccatgacgg ccttccacga atcagccgcg aagtaacacg ccatgcccga
     2101 ccggacaatt cgcgtgcagt actacgcgct attgcgcgag caggcgggcc gcagcgacga
     2161 aaccctcacc accagcgcgc gcgacgcgcg cgagctctac atggagttga gccagcgcta
     2221 cgcgttcacg ctgccggccg aaatgctgcg ggtggccatc aatgcggaat ttggcgagtg
     2281 gtcgcagccg ctcgaggctg gcgatgcggt cgtcttcatt cctccggtgg cgggcggatg
     2341 aacgcgttcc ggttcagcac cgccccgctg gacaccgctt cccttcagcg tgaaatgcgc
     2401 gacgtgacct gtggcggttt cgcggctttt gaaggctggg tgcgcaacca caacgagggc
     2461 ctggccgtca cgcgcctgga atacgaagcc tttgccgagc tcgccgagaa ggaaggtgcg
     2521 cgcatcgtgc tggaagcaat tgcgcggttc ggcgtcacgc gcgccggctg tgtgcatcgc
     2581 atcggctcgc tggccctggg cgaggtggcc gtgtgggtcg gcgtgagcgc cgcgcatcgc
     2641 gacgaagcgt tccgcgcgtg ccgttacatc atcgatgaag tcaaacaccg cgtgccgatc
     2701 tggaagaaag agcactacga gaacggcgac tccggctggg tgaactgtga gcgttgcgcc
     2761 gcgccagcgg cgcaccacga ggaatatgca gcgccggcgg cgcacgaacg tgactacgcc
     2821 gctcccgtca actatctcca cgaacacgcg cacgcgcacg accacgacca cgatcatgag
     2881 attccttcgg tcgccgcgcc tgactactcg cgccagatcg cgctgcgtga ggtcggcccg
     2941 gagggccagg cgcgcctgcg cgccgcatcc gtactggtcg tcggcgccgg tggactgggc
     3001 gtgccggtgc tgcagtacct ggccggagcg ggtgttggcc gcatcggcat cgtcgacgcc
     3061 gaccggctcg aaccgtcgaa cctgcatcga cagacctggt atgcgctcgc cgattgcgga
     3121 ctggagaaat ccacgctggc ggcggagcgc gtacgcgcac tgaatcctga agtacgcgtc
     3181 gagccgcatg cgctgcgtct ggacgccaac aatgccgatc ggctggccgc cggtcacgac
     3241 ctgatcctcg attgcagcga caacttcgcg acgaagttcc tgctcaacga cctggcgttg
     3301 cgcacccgca agccggtgct gttcgcaagc gtctaccagt acgaagggca actccaggtg
     3361 gtgcgcggcg acgaggccac agcctgcctg cgctgcgtgt ggcccgaagc gacgcgtgat
     3421 ggcctggtgg gtaattgcgc cgaagccggt gtgctgggcc ccgtgcctgg tgtgttcggc
     3481 agtctccagg cgctcgaggc gctcaaactg ttgttaggac tgccgggcct cgagccgggc
     3541 gaaatgctga ttttcgacct ggtgagcctc aacacgcagc gtctgcgcgc ccgacggacg
     3601 ccggactgcg atgcgcatcg gcgcgccggc cagtccgccg caaccctctc acccctcgaa
     3661 ctcgaagtgc agttcggctc gctcgccgaa gcccaggcag ccggatttac cttcgtggac
     3721 gtacgcgatt cgcgcgagcg tgccgacgat ccgctgccgt cgctcgcact gtgcctgccc
     3781 atgtccaaac tactcacgga cgcggcgatg ctcgacaccg gcgggcgcta cctgctcatc
     3841 tgcgccacgg gcaagcgcag cgccgcggcg gccgacctgc ttcgctcgca gggctttcgc
     3901 gagtgccgct cacttcgtgg tggcatcaag ggattgaaag cctccgcgtg aaaagcacgc
     3961 tgacgttcgt cgtcgcctgc ctgctggcat tgccggccgc cggcgctcgc gcggaagtgg
     4021 agtccgccga ccatcccttg ctcgatctcg cgcgcgaatt gcagaagaag cgcaaaccgg
     4081 acttgcgcgt agttcccgga gcaccggggc tgcggcaggc cgaacacgcg ctggcccagg
     4141 cgaaggcgcc cgccgacgac aaatgcgctg gtggcatcgg cgcgttgcgc ttcgcggaac
     4201 tgcacgcgga tctcgcgctc gcacgcaccg ggcaaggcga ttttgccggc gcggccagcg
     4261 catatcgcag cgcgcatgcg tgccggccgc gcgacccgca gatcctggcg gcgctctccg
     4321 gtgtcctgtt cgacgctcgc gactacgtcg ccgcgcgcga ggccatcgac gccgcgctcg
     4381 caattgcacc gcgtgcagtg aatgccaatc gcctggcggg gaacatcgac ttcgtcgaag
     4441 agcgctgggc aaacgccacc ggacgtttcc gctacgtggc ttccagcgat ccggaccgcg
     4501 tgcaggccgg ttacggccaa ctcatgttct ggctgtcgca gatgcgcgcc ggtgtgccca
     4561 agcccgaatt ggtggaacgc acgcccggcg aaggatggcc gcagccgctc ctgctctaca
     4621 tgcggggcca gtacaccgaa gccgagctca cggtcccggt gcggcgtggc gacgacgaag
     4681 acaatatcca gcccaactcc agcaccgacg agcgattgtg cgaggcgttg ttctacgtcg
     4741 gcgaagcgta ctgggcgcga gggcagttgg acgtggcgcg cgattatttc gcggcgctgg
     4801 tgaacatcaa ggtcatctac tttctcgaac acgggctggc gctggccgag atcgcgaagc
     4861 tgagggagct gacgcggtaa tggggacagg ctcgcgttag cggccgttcc tgatttgcca
     4921 gcaaaagggg acggggctcg gtggtggagc gtcggcgcgt gaagaaggac aggcttccgg
     4981 cgctcactct cgggtgcgct tgagtagtag aaatggagga ccatttctat tactcgcgtt
     5041 cgcttgctcg gaatcggagc ttggaccctg ccgcatgcgc ccggaaatga ggcgcggtcg
     5101 ttacgggcgt atttgaccgg tgccccgtac cacgtacttg tacgaggtga gctgctccac
     5161 gccgaccggc ccgcgcgcat gaaagcggtc ggtggagatg ccgatttccg ctcccatgcc
     5221 gaactcgccg ccatcggcga attgcgtgga cgcgttgtgc aacacgatgg cgctgtccac
     5281 gcggtcgagg aagcgctgcg cggtgacggc attggcggtg atgatgctct cggtgtgcgc
     5341 cgaaccgtac ttgccgatgt gttcgatggc cgcatcgacg ccgtcgacca cgcgcgcggc
     5401 gatgatggcg tcgagatatt cggtgtacca gtcctgctcg accgagggct tcacgcgcgc
     5461 gtcgaccttc tgcacggtgg catccccgcg cacttcgcag ccggcgtcca gcaggtcctt
     5521 gaccacgggc gccaggtgcg tggccgcgca ggcggcgtcc actaacaagg tttccgccga
     5581 gccacacacg ccggtgcgac gcagcttggc gttcgtgacg atggcgcgcg ccatggcgag
     5641 atcggcttcg cgatcgacat acacgtgaca cacaccttcg aggtggccga tgacgggtac
     5701 gcgcgcttcc tgctgcacgc gcgcgaccag gctcttgccg ccgcgtggca cgaccacgtc
     5761 gatgtaatcc gtcatcgacg acagcaggta accgacggcg gcgcgatcgg tggtcggaac
     5821 cagttgaatg ctcgcagccg gcagaccggc ggcgctgagt ccgtccacga ggcaggcgtg
     5881 aatagcgcgg ctggaatggc tgctttcgga accgccacgc aggatggccg cattgccgga
     5941 tttcaggcac aacgaccccg cgtcacaggt cacgttgggc cggctctcat aaatgatgcc
     6001 gatgacaccg agtggcactc gcacgcgcgc aatgtcgagg ccattggggc gggtccactg
     6061 cgcgatcgtg gcgccgatcg gatcggcgag tgcggctatt gcctccacgc cctgcgccat
     6121 cgcctcgatg cgaccggcgt cgagcgcgag gcgatcgagc atcgcgcccg acaggccctt
     6181 ggcgcgcgcc gcctccatgt cgcgggcatt cgccgctaac aactccgcct tgcgggcgcg
     6241 caacgagacc gcgatctgac tgagcgcccg gttcttcgtg gcggtgctcg cgagcgccaa
     6301 cacggccgcc gctgccgccg cgtcccgccc gagccggtcc atgatggcgg cgatttctgt
     6361 cgtggaatcc gtattggcgg cggcgttcaa agggtttccc ggagattgtc aggcggaagc
     6421 gagatcgggg cgcaacagga ccagatcgtc gcgatggatc atttcggcac gaccgcggaa
     6481 tccgagcagc gcctcgatgt cggcgctgcg cttgccgatg atgcgggagg cgtcccgatc
     6541 gctgtaggcg atcataccgc gcgcaatttc cgcgccatcg gcgtcgagca cgctcacggt
     6601 gtcgccacgc ttgaatctgc cgctgaccgc ggtcaccccg gcgggtagca ggctcttgcc
     6661 tgaccgcagc gcctgcagtg cgccggcatc gatcgtgatt gcaccgctcg ggcgcagcga
     6721 ccctgcgatc cactgcttgc gcgcgttttc gggattggac gcgggcagga accagctgca
     6781 gtcggcgccc tcctcgaggc ggcgcaccgg atggagtggg ccacccaggg aaatggccat
     6841 ggcgcagccc gccgacgtgg caatctttgc cgcggcgatc ttggtggcca tgccgcccga
     6901 gcccacggca gatgccgagc cgccacccat cgcttcgatt tcgggcgtga tgtcgcggac
     6961 ttcggggatc agccgcgcgg tggggtcgag gttcggatct gcggtataca gcccggccac
     7021 atcggacagc agcaacaggc agtcggcgct cgtcatctgc gcgacacgag ccgcgagacg
     7081 gtcgttgtcc ccatagcgga tctcggtggt cgcgaccgtg tcgttttcgt tgatgactgg
     7141 cagcgcgccg agctccagta gctcggagag cgtggcacgg gcattgagat aacgccgccg
     7201 gacctcgctg tcttccagcg ttaacagaat ctgggcgacg gtgacgccgg agcgggcgaa
     7261 cagctcctta tagatgtgtg ccagagcgat ctggcccacg gccgcggcag cctggctcag
     7321 ctccagcttc aacgcacccg gtgcgagctt cagctcgcgc cggccgagcg cgatggcgcc
     7381 ggaagacacg agcaccacct cctgcttgcg cgcacgcagc cgcaacaggt cttcgaccag
     7441 gctctcgagc cagggccgat tgacctgtcc gctggactgg tcgacgagca gcgccgaacc
     7501 gactttgacg acgatccggc gtgcacgagt tagaggtgat gctgtagtca ttaacggggc
     7561 gatcttacgc aatacatccc gttgccgcat tcgcaagcac accctaaact ctcgcccctc
     7621 caccagaggc aacgacttgt gagcagagaa tacgcaccga ccccgggcaa gtggtacgag
     7681 aacaaggacg aggaagaaac cttccgcgtg ctgtccgtag acgaggacga cgagctcgtg
     7741 gaaatcgagt acgaggacgg cgagatcgaa gagctcgacc tcgacacctg gcatgagctc
     7801 gatctcgagc tgaccgacgt gcccgagggc tggtccgatt ccgccgtcga cgacgatgac
     7861 gatgaggacg aagacttcga cgaggatgaa gacgacgatg atgatgacga cgatgatgac
     7921 gacgacgacg acgacgaaga agaaagctac tagttagcat ggtcaccggc gccgccgaga
     7981 gctggtacca cgaaaaggaa tccgcctggc tctacggtgt agtggccgcg gccgaacagg
     8041 atcccgtaaa acgggcgttg ttcgtgaaac tcgccgccgc cgctgaagcg caggcggcgt
     8101 gctggctggc aacccgtccc gaactgaagc ccgtgttttc gcccgcgctc agggcgcgca
     8161 tcgtcgcgcg cctcgtgcgg cagatcggac ccaggcgcct gcgcggcgtg ctggccgcca
     8221 tgaagctgcg cgggctgtcg gtatattcgg gctcccacgc acccgccccc catccgatgc
     8281 cgacgacggt tgccgaaatt ggcaagcggc attcctcggc gggaggcaac cttcgcgccg
     8341 ccgtgttcgg cgccagcgac ggcctcgtct ccaatgcggc gctggtcatg ggtgtcgcag
     8401 ccgcaagttc cgatctgcat tccgtgcaga tagcgggagc cgccggattg ctggccggcg
     8461 cgctttccat ggccgcgggc gaatacgttt cagtgcgctc gcagcgggag ttttacgagc
     8521 accagatgga cctcgagcgc aaggaactcg cggaatatcc ggaagccgag gctgaagagc
     8581 tggcgctcat ctataacgcc cgcggcatcg acatggacga ggcgcgccgc atcagcgcaa
     8641 tcctgctttc gcgccccgaa caggcgctcg acgtgctggc gcgtgaagaa ttgggcctca
     8701 gtcccgacaa tctcgggtcg ccgttcgcgg cggcctggtc atctttcctg tcatttgcct
     8761 ccggcgcgac gat
//
LOCUS       DASQJS010000046         4940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2225428, whole genome shotgun sequence.
ACCESSION   DASQJS010000046 DASQJS010000000
VERSION     DASQJS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2225428"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1789
                     /locus_tag="VEW08_01865"
     CDS             <1..1789
                     /locus_tag="VEW08_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ39503.1"
                     /translation="ETKSLELGSTPERSTESVLRAVCLRLGCTAATAVFPREDSRAVW
                     SRSEEDAAFARQQLSIVGDELARRAIETKKPVATNKLKHVSGGQIACKLVAVPLIARG
                     GPAGALIIFNRPEEPSFDEFSVRRLGRFARLLARNATQDLDSLTGLLSWDGFKTRVDT
                     WQRNAPPGAMVSMLYGDIDCLHLINDLAGFSDGDRVIARCGASIRHALVGADSFACRL
                     SGDRFTVFLPHMNLAQTRQLADRICKDVSNAPAVKQEPNHPVSMSWGAVSTSACDLHL
                     DHRIAEAEIACKTAKDRGRGRVEVYQDTDQSMIRRHDEIGLIGSVREALRLERIVVYA
                     QPITPLINHSLPTTYELLVRIVDAKGNIVEAADFMSAATRYQILPEIDRAVCERAFEQ
                     LSKNTSRAGPLRVSINLSGPTLSDPEFLEWLLASMAKYGIDGTCLVFEITEAAAAANM
                     QQVQQFIRRLSTHGVRFALDDFGTGVSSLAYLKDLEINTIKLDGSYVVDVLTNPRSQA
                     LVRAIVQLADAMGITTVAEYVESNTVRDRLASLGIQFGQGFALGRPGPLDDVLAAVAD
                     RAPEGRERPFTERKTYTTPSGIPIMFKHA"
     gene            1857..3167
                     /locus_tag="VEW08_01870"
     CDS             1857..3167
                     /locus_tag="VEW08_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ39504.1"
                     /translation="MIKLTRYQWTVFFAAWLGWGFDIFDGLLFNFVAPNAIPTLLDIP
                     IGGPEAKAAVPYWTAILNSILLLGWAAGGVIFGPICDRFGRRRVLMLTMVLYAVGTAS
                     CAFVTEMWQLVIFRIVASLGIGGEWAAGSAMVAEVVPEEKRVEAGALLYTSAPFGLFL
                     ADFVNTEVAGNWFIHDPSMSWRYVLMFGLVPAAVAFIVRAFIKEPERWAAAAATAAPA
                     RVREIFAPDMRARTISALIVAVTALITWWTCNAFIQAMSNSLAGAEATRLAMDATATA
                     VLKRDWMTLAMYSFCWGGLLGTLLTVPLAKMLGRRKMYAIYFAGSALALLATFGLDLP
                     PETRLRMYFFIGLTVFGVFGSFTFYLPELYPTRLRSTGSGFCYNIGRVVTAGGVFVVG
                     MIQNAAKGDPEVFLRTMFMIGFVPIIGLLLFHWVIETRGQKMPD"
     gene            complement(3174..>4940)
                     /locus_tag="VEW08_01875"
     CDS             complement(3174..>4940)
                     /locus_tag="VEW08_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39505.1"
                     /translation="WQRGTTLATLRYPGGLAISGNRILIGGNQCQDKAFIYQRAATGV
                     WGLTGRIAAGGGLCSTAPTPVELNYDYALVRISARFVGVWLPSGSLDWTLAGQIQIPE
                     GASTSPGAMALQKSTAVSPGSAVFRRTGTAWGYMGQLQPVDYGNGTGHANAVEYRDGV
                     LLANEGWSEIHQYTKPYVYLENAGGTFDHVGILETSGFTQDFDISKGTVVASFENIGE
                     TGVDVFNLPAPVRPLPSITNDFNARDISGFQQTAGSQFALAGNVYNYLYRQSSATTEA
                     VAVLTNSDWTHYQSVEADITPTAFNSPNSYVGLAVRYLDIGNNYYVTWRVSNLIQLKR
                     KVNGAFVTLAEKPLALPLNTPHHLRLSVAGSLLSVDVDGQQVLSATDTTFSHGRAALI
                     TNRARADFDNVSASPTAPLNINYNDWALYWYGFGRPFTELGGNWEITGQEDPEGLSQT
                     TTTGLALAYNGVATENQVVRANMRLDSFAGSTSGAWFGLLARYVDEQNHYFLSVRSSN
                     QLQIRRIVNGVTTVLKAVSFTAAPGAMREYTLSVRGNELHAFVDGQLVATAIDDSLPR
                     GKYGMGTYRAAATWQDFEVTQP"
BASE COUNT          849 a         1567 c         1591 g          933 t
ORIGIN      
        1 agaaacgaaa tcgctcgaat tgggctcgac gcccgaacgg agcactgaaa gcgtattgcg
       61 tgcagtgtgt cttcgattgg gatgtacggc cgcgacggcg gtgttcccgc gcgaggattc
      121 gcgcgccgta tggagccgct ccgaagagga tgccgcgttc gcccgccagc aattgtcgat
      181 cgtcggtgac gaactcgcgc gccgcgccat cgagaccaag aagccggttg ccacgaacaa
      241 actcaagcac gtgagcggcg gccagatcgc ctgcaagctg gtcgcggtac cgctcattgc
      301 gcgcggcggc ccggccggtg cgctcatcat tttcaatcgc ccggaagaac cgagcttcga
      361 cgaattctcc gtgcgccgcc tcggccgctt cgcgcgcctg ctggcgcgca acgcgacgca
      421 ggatctcgat tcactcacgg gcctgctgtc gtgggacggc ttcaagacac gcgtggacac
      481 ctggcagcgc aacgcgccac cgggcgccat ggtgtcgatg ctgtacggcg acatcgattg
      541 cctgcatctg atcaacgatc tcgccgggtt ttccgacggc gatcgcgtga tcgcgcgttg
      601 tggcgcctcg atccgccatg cgctggtcgg cgcggacagc tttgcgtgcc gcctgtcggg
      661 cgaccggttc acggtgttcc tgccgcacat gaatctcgcg cagacccgcc agctcgccga
      721 tcgcatctgc aaggacgtca gcaatgcgcc cgccgtgaag caggagccta accacccggt
      781 ttcgatgtcg tggggcgccg tgtccaccag cgcctgcgac ctgcatctcg accaccgcat
      841 cgccgaggcc gagatcgcct gcaagacagc caaggaccgc ggccggggcc gcgtcgaggt
      901 ctaccaggac accgaccaga gcatgatccg ccgccacgac gagatcggac tcatcggcag
      961 cgtgcgcgag gcgctgcgcc tcgagcgcat cgtggtgtac gcacagccga tcacgccgct
     1021 gatcaaccac agcctgccga ccacgtacga actgctggtg cgcatcgtcg acgccaaggg
     1081 aaacatcgtc gaagccgccg acttcatgtc cgcggcgacg cgctaccaga ttcttcccga
     1141 gatcgatcgg gcagtctgcg agcgggcctt cgagcagctc agcaagaaca ccagccgcgc
     1201 cggtccgctg cgcgtgtcga tcaatctttc cggcccgacg ctgtcggatc cggaattcct
     1261 ggagtggctg ctggcctcga tggccaagta cggcatcgat ggtacctgcc tggtgttcga
     1321 aatcaccgaa gcggccgcgg ccgccaacat gcaacaggta cagcagttca tccggcggct
     1381 cagcacgcac ggcgtgcgct tcgcgctcga tgacttcggc acgggcgtga gttcgctggc
     1441 ctatctcaag gatctcgaga tcaacacgat caagctcgac ggctcgtatg tcgtggacgt
     1501 gctgaccaac ccgcgctcac aggccctggt gcgcgccatc gtgcagctgg ctgacgccat
     1561 gggcatcacg accgtcgccg agtacgtgga gtcgaacacc gtgcgcgacc ggctggcgtc
     1621 gctcggcatc cagttcggac agggttttgc gctcggccgt cccggcccgc tcgatgacgt
     1681 gctcgccgcg gttgcggatc gcgcgccgga agggcgcgaa cgtccgttca ccgagcgcaa
     1741 gacgtacacc acgccgtccg gcataccgat catgttcaag cacgcgtagg ggcataaatg
     1801 gtgtcaggca ccgtttgcta ggaaacggtg cctgacccca tttacactcg cgcgccatga
     1861 tcaaactgac gcgctatcaa tggacagtgt tcttcgcggc ctggctcggc tggggcttcg
     1921 acatttttga cggactgctg ttcaacttcg tggcgcccaa cgccattccc acgttgttag
     1981 acatcccgat cggcgggccg gaagccaagg ctgctgttcc atactggacg gccatactca
     2041 attccatcct gctgctgggc tgggccgcgg gtggtgtgat cttcggcccc atctgcgacc
     2101 gcttcggacg caggcgcgta ctgatgttga cgatggtctt gtacgcggta ggcaccgcca
     2161 gctgcgcatt cgtcaccgaa atgtggcagc tcgtcatctt ccgcatcgtc gccagcctcg
     2221 gcatcggcgg cgaatgggcc gcgggctcgg ccatggtggc cgaggtagtg cccgaggaaa
     2281 aacgcgtcga agctggcgcg ctgctgtaca ccagtgcgcc attcggcctg ttcctcgctg
     2341 atttcgtgaa cacggaagta gccggaaact ggttcatcca cgatccatcg atgtcgtggc
     2401 ggtacgtact gatgttcgga ttggttccgg ccgccgtcgc gttcatcgtg cgcgccttca
     2461 tcaaggagcc ggaacgctgg gctgcggccg ccgccacggc ggcgcctgcg cgcgtgcgcg
     2521 agatcttcgc gcctgacatg cgcgcgcgga cgatcagcgc gctgatcgtc gccgtgactg
     2581 cgctgatcac ctggtggacg tgcaatgcgt tcattcaggc catgtccaac agccttgcag
     2641 gagccgaagc caccaggctg gccatggatg cgacggcaac cgccgtgctc aagcgggact
     2701 ggatgacact ggcgatgtac agcttctgct gggggggatt gctgggcacg ctgctgaccg
     2761 tgccgctggc caagatgctt ggacgacgca agatgtatgc gatctatttc gcgggctccg
     2821 cgctcgcgct gctcgccacc ttcggcctgg acctgccgcc ggaaacgcgc ctccgcatgt
     2881 acttcttcat cggcctcacc gtcttcggcg tgttcggcag tttcacgttc tatctaccgg
     2941 agctctatcc cacgcgcctg cgctcaacgg gcagcggctt ctgctacaac atcggccgcg
     3001 tggtgaccgc cggcggcgta ttcgtcgtcg gcatgatcca gaacgctgcc aagggtgatc
     3061 ccgaagtctt cttgcgcacc atgttcatga ttggcttcgt gcccatcatc ggattgctgc
     3121 tgtttcactg ggtcatcgag acgcgcgggc agaagatgcc ggactgatgc gattcatggc
     3181 tgcgtgactt cgaagtcctg ccacgtcgcc gcggcccggt aagtgcccat cccgtacttg
     3241 ccgcggggca gggagtcatc gattgccgtc gcgaccagtt ggccatcgac gaatgcatgc
     3301 agttcgttgc cgcgcacgct cagggtgtac tcgcgcatcg cgcccggcgc ggcagtgaac
     3361 gacacggcct tgagtaccgt ggtcacgcca ttcacgatcc tgcggatttg cagctggtta
     3421 gagctgcgga cggacaaaaa gtagtggttc tgctcgtcca cgtaacgagc gagcaatccg
     3481 aaccaggcgc ctgacgtcga gcccgcgaag gaatcgaggc gcatgttcgc gcgaactacc
     3541 tggttctcgg tagcgacgcc gttgtaggcg agcgcgagac ccgtggtggt ggtctgtgag
     3601 agaccttcgg gatcttcctg gccggtgatc tcccagtttc cacccagctc ggtgaagggc
     3661 cgcccgaagc cataccagta gagcgcccag tcgttgtagt tgatgttcaa cggcgcggtc
     3721 gggctggccg atacgttgtc gaagtcggcg cgagctcggt tcgtgatgag cgcagcgcgg
     3781 ccatgcgaga acgtcgtgtc cgtcgcggaa agcacctgct ggccgtcgac gtcgaccgag
     3841 agcaacgacc cggccaccga gagccgcagg tggtgcggcg tattgagcgg cagggcaagc
     3901 ggcttctcgg cgagcgtcac gaacgcgcca ttcaccttgc gcttgagctg gatgaggttg
     3961 gagacgcgcc aggtcacgta gtagttgttg ccaatgtcga ggtagcgcac ggcgagaccc
     4021 acgtaactgt tcgggctgtt gaacgccgtg ggcgtgatgt cggcttcgac ggattggtag
     4081 tgagtccagt cactgttcgt cagcaccgcg acggcttcgg tggtcgccga cgactgccgg
     4141 taaaggtagt tatagacatt gcccgcgagc gcgaactggc tgccggcggt ctgctggaag
     4201 ccgctgatgt cgcgcgcatt gaagtcgttg gtgatcgagg gtagtggccg tacaggagcg
     4261 gggagattga agacgtcgac gccggtctcg ccgatgttct cgaaactcgc gacaaccgtt
     4321 cccttgctga tgtcgaagtc ctgggtgaac ccggaggttt ccaggattcc gacatggtcg
     4381 aatgttccgc cggcgttctc gagatacacg tacggcttcg tgtactgatg aatctcactc
     4441 cagccttcgt tcgcgagcag cacaccatcg cgatattcga ccgcgttcgc atggcccgtg
     4501 ccgttgccgt agtccacggg ttgcaactgg cccatgtagc cccacgcggt gcccgtgcgc
     4561 cggaagacag cggatcccgg ggacacggcc gtcgatttct gcaacgccat ggcgccgggt
     4621 gaagtgctgg cgccctcggg aatctgtatc tgtccggcca gcgtccagtc gagcgagcct
     4681 gaggggagcc acacgccgac gaagcgcgcg ctgatgcgca ccagcgcgta gtcgtagttg
     4741 agttcgaccg gcgtgggtgc ggtgctgcac aagcctccgc cggcggcaat tcgcccggtg
     4801 agaccccaga cccccgtcgc ggccctctga tagatgaacg ctttgtcctg gcattgattg
     4861 ccgccgatca gaatgcggtt gccggaaatg gccagtccgc ccggataacg cagggtcgcg
     4921 agcgtggtgc cacgctgcca
//
LOCUS       DASQJS010000047         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1596716, whole genome shotgun sequence.
ACCESSION   DASQJS010000047 DASQJS010000000
VERSION     DASQJS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596716"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..1725
                     /gene="pepQ"
                     /locus_tag="VEW08_01880"
     CDS             379..1725
                     /gene="pepQ"
                     /locus_tag="VEW08_01880"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: protein motif:HMM:NF010133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HYJ39506.1"
                     /translation="MNSPAIRQNVDSWPRLFTEHLEIVTKRSASALQSAGYDSLLVHA
                     GTPPLLFLDDHHLPFRVQAPFKVWAPLSDAPDSFVYFAPGKKPVLLIHQPVDYWHKSP
                     EMPNDYWTSAFDIVSCADRTSARRALPADLSRTAFIGAPFPELLGWGPAAINPEHLIA
                     QLDYGRAAKSAYEVACLHKANVLGARGHVAAEQAFRAGATEFEIALAFMKACGQRERE
                     LPYNPIIALNEGGAVLHYQIQQRTKPAKLHSLLIDAGAEFGGYASDITRSHSYQDAEW
                     AGLIRQFDELQLKLCAQVRAGLDWRDFHQASYRAISEFLREAGVINVSADEAVDSALT
                     SVFYPHGIGHLLGLQVHDVGGLQGTPEGKQIERPYNHPFLRLTRKLDDGCVVTVEPGF
                     YFIDQLLDEAKSKPIGKMIEWKRVEQLKKFGGVRIEDNVVARPGGPDNLTRAAFNA"
     gene            complement(1754..2101)
                     /locus_tag="VEW08_01885"
     CDS             complement(1754..2101)
                     /locus_tag="VEW08_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39507.1"
                     /translation="MATVRYTWMFRTAAAVFVFFGGVWLWRFGLTDYQPQYRPYGLVA
                     GVLALLVAIFLLRGNRVAIGFSAVAAGLVGISAAVFAPNAGGPGILFLAALAISCVIY
                     AVLAARVLLERRS"
     gene            complement(2124..>2419)
                     /gene="mscL"
                     /locus_tag="VEW08_01890"
     CDS             complement(2124..>2419)
                     /gene="mscL"
                     /locus_tag="VEW08_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HYJ39508.1"
                     /translation="SLVEDVFMPPLGKVIGGVNFTDLAVSLGMGPDGKTEVLWKYGNF
                     LQIVFQFLIVALVLFMVIRTLNKLKKPDPAAPAPTAPRQEVLLTEIRDLLAKK"
BASE COUNT          504 a          752 c          738 g          425 t
ORIGIN      
        1 gccgacgagg cagcagcgtc ggcagcagga gcttccgcat ccttcttggc gcagccagtc
       61 atcagcgcga aagccgcgat cgaggcggcg agagcaacat tcaatttcat gtaggggtac
      121 cccaaaaata tctagtggta agagagacca aacaaacgtt tgttgccgca atttgcattg
      181 cggcgcgcga ttttatcgat tccccaatgt atttgaaaca agtgcaattt ttgtccgttg
      241 atggcgacag tgagtcctca cgggcggcaa gcccgagcga cgcgaatctc gaggtttgct
      301 cacaccgggc ggcaggaagc gcaatatgtt gcgctgcatc aacaggttgc gcatcgtcgc
      361 tacgcataga atcgactcat gaattcacct gctattcgac agaacgtgga ctcctggccc
      421 cggcttttta ccgagcacct cgagatcgtg acgaaacgca gcgcctctgc cctgcagagc
      481 gctggctacg acagcctgct ggtgcacgct ggaacgccgc cgctgctgtt cctcgacgac
      541 catcacctgc cttttcgcgt ccaggcgcca ttcaaggtat gggcgccgct cagcgatgcc
      601 ccggattctt tcgtgtactt cgcgcccggc aagaagcccg tgctgctcat ccaccagccc
      661 gtcgactact ggcacaagtc gccggagatg ccgaacgact actggacgag cgccttcgac
      721 atcgtgagct gcgctgatcg cacatccgca cggcgggcac tacccgcaga tctgtcgcgc
      781 accgcgttca tcggcgcacc gttccccgag ctgctcggct ggggtccggc ggcgatcaat
      841 ccggaacacc tcatcgcgca gctcgattac gggcgtgccg ccaagtcggc atacgaagtc
      901 gcctgcctgc acaaggcaaa cgtgctcggc gcccgcgggc atgtggctgc ggagcaggcg
      961 tttcgcgccg gcgccaccga gttcgagatc gcgctcgcgt tcatgaaggc ctgtggccag
     1021 cgcgaacgcg aactgcctta caacccgatc atcgcgctga acgaaggcgg cgcggtactc
     1081 cactaccaga tccagcagcg cacgaagccg gcgaaactgc attcgctgct catcgatgcc
     1141 ggcgccgagt tcggcggata tgcctcggac atcactcgca gccattcata ccaggacgcc
     1201 gagtgggcgg ggttaataag gcagttcgat gagctgcagt tgaagctttg tgcgcaggtg
     1261 cgcgccgggc tcgactggag agactttcac caggcgtcct accgggccat cagcgagttc
     1321 ctgcgcgaag ccggcgtgat caacgtgagc gcggatgaag ccgtcgacag cgcgctgacc
     1381 agcgtgttct atccgcacgg catcggccac ttgttaggcc tgcaggtgca tgacgtcggt
     1441 ggcctgcagg ggacgccgga aggcaaacag atcgagcgcc cgtacaacca tccgttcctg
     1501 cggctcacgc gcaagctcga tgacggttgc gtcgtgacgg tggaacccgg gttctacttc
     1561 atcgaccagt tgctggacga ggcgaagtcg aagccgatag gaaagatgat cgaatggaaa
     1621 cgggtcgagc agctgaagaa gttcggcggc gtgcgcatcg aggacaatgt cgtggcacgt
     1681 ccgggtgggc cggacaatct cacgcgcgcg gctttcaatg cgtaaggtgg cgtagccgca
     1741 agatctgggc ccttcaggat cggcgctcta acagtacgcg cgccgcgagc accgcgtaga
     1801 tgacgcagga gatcgccaga gccgcgagga aaaggatgcc gggcccacct gcattcggcg
     1861 cgaataccgc cgcggagatg ccaaccagcc ccgcggcaac tgcggaaaat ccgattgcca
     1921 cgcgattgcc tcgcagcagg aatatggcga ccagcagcgc cagcactccc gccaccagcc
     1981 cgtagggccg gtactgcggt tgatagtccg tcaggccgaa tctccagagc cacacgccgc
     2041 caaagaacac gaatacggca gccgccgtcc ggaacatcca ggtgtagcga acggtggcca
     2101 tcgaggttca gcgaattgac taactatttc ttcgcgagca gatcgcggat ttccgtcagc
     2161 agcacttcct gacgtggtgc ggtcggcgcc ggggccgcag gatccggctt cttcagcttg
     2221 ttgagcgtgc gaatgaccat gaacagcaca agggcgacga tcaggaactg aaagacgatc
     2281 tgcaggaaat tgccgtactt ccacagcact tcggtcttgc cgtccggacc catgccgagg
     2341 ctaacggcga ggtcggtgaa gttcactccg ccaatcacct tgcccagcgg cggcatgaac
     2401 acgtcttcca ccagcgaag
//
LOCUS       DASQJS010000048         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2225768, whole genome shotgun sequence.
ACCESSION   DASQJS010000048 DASQJS010000000
VERSION     DASQJS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2225768"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /gene="rlmKL"
                     /locus_tag="VEW08_01895"
     CDS             <1..1480
                     /gene="rlmKL"
                     /locus_tag="VEW08_01895"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HYJ39509.1"
                     /translation="HDRAVWEKVKHDALARELKPSLRLRGVDADASVLAAARENAARA
                     GLGALIQFSSGRLADAKPAGEGPGENKGFLATNPPYGVRLEDRETARALMKQLGDVLR
                     ERFSGWDAVILAGSPDAGLELGIRAERVHTVWNGALECRLLRLHVSAETEKQMLHTGR
                     GARIDESLAESPGSKMFGNRLAKNIKQLKGWAEREGVTCFRLYDADMPEYSFAIDRYT
                     EFDGANTWLYVQEYAAPKTIEPEAVQRRRGEALAALPGVTGIPVEKIHLRQRRRTTRG
                     DQYEKLAESADFKLVAEGGLRFWVNFTDYLDTGLFLDHRVTRERLRADAASRRFLNLF
                     AYTGSATVYAAAGKARATTTVDMSATYLDWAQRNLAVNGFAEREHEFIQADCITWLRN
                     AAEDRRVYDLVFLDPPTFSNSKRMDDILDVQRDHAGLIDSCMALLAPGGKLVFSTNAQ
                     KFKLDGMLGERYKVTDISRATLPKDFERNPRIHQCYELESGK"
     gene            1477..1899
                     /locus_tag="VEW08_01900"
     CDS             1477..1899
                     /locus_tag="VEW08_01900"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008008030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYJ39510.1"
                     /translation="MIKKGSAVWKGTLKEGTGSISTETGVLKNAPYGFKARFEDGPGT
                     NPEELIAAAHAACYSMALSAGLGKEGFTADSIETSAAVRLEKVGDGFAITHSDLICLA
                     KVPNITEAKFAEIAEITKSGCPVSKVLNAKISLVAKLT"
     gene            complement(1900..>2194)
                     /locus_tag="VEW08_01905"
     CDS             complement(1900..>2194)
                     /locus_tag="VEW08_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39511.1"
                     /translation="ALTIDVDPGDPEELRDANLDERTDVELHALNAKGVPVTLEIRHY
                     AYVHTRAKISKSSQRAFRKYGDFAWRLRVPANGRKVLAYRFEQPSAPDTDDDE"
BASE COUNT          437 a          678 c          702 g          377 t
ORIGIN      
        1 tcacgaccgc gccgtgtggg aaaaagtgaa acacgatgcg ctcgcgcgcg agctgaagcc
       61 ttccctgaga ttgcgcggtg tcgatgcgga cgcatccgta ctggcggcgg cgcgtgaaaa
      121 tgccgcacgc gccggcctgg gtgcgctcat tcagttcagc agcggccggc tggccgatgc
      181 aaaacccgcg ggagaaggcc caggcgagaa caagggcttt ctcgccacca atcctccgta
      241 cggagtgcgc ctcgaagacc gcgaaaccgc gcgggcgttg atgaaacagt tgggagacgt
      301 tctgcgcgag cgcttttccg gctgggacgc ggtgatactc gcgggttccc cggatgcagg
      361 cctcgagctc ggcatccgcg ccgagcgcgt gcacacggtg tggaacggcg ccctcgagtg
      421 ccgcctgctg cgcctgcacg tttcagcgga aacggaaaag cagatgctgc acacgggccg
      481 tggcgcgcgc atcgacgaat cgctggcgga atcgccgggc tcgaagatgt tcggcaaccg
      541 tctcgcgaag aacatcaagc agctcaaggg ctgggcggaa cgcgaaggcg tgacatgctt
      601 tcggctgtac gacgccgaca tgcccgagta ttccttcgcc atcgatcgat ataccgagtt
      661 cgatggagcg aatacctggt tgtacgtgca ggagtacgcg gcgccgaaaa ccatcgagcc
      721 cgaggcggtg cagcgccggc gcggcgaagc cctggcggcg ctgccgggcg tcaccggcat
      781 acccgtggag aaaatccacc tgcgccagcg ccggcgcacg acgcgcggtg accagtacga
      841 gaagttagcc gagagcgccg acttcaagct cgtggcggaa ggcggtttga ggttctgggt
      901 caacttcacc gactatctcg acacgggcct gttcctcgat catcgggtca cgcgtgaacg
      961 gttgcgcgcg gatgccgcca gccgccgctt cctgaatctc ttcgcctaca cgggcagcgc
     1021 aacggtgtac gccgcggccg gcaaagcccg cgcgaccacg accgtggaca tgtcggccac
     1081 ctacctcgat tgggctcagc gaaatctggc agtgaacggc tttgcggaac gcgaacatga
     1141 attcatccag gccgactgca tcacctggct gcggaacgcg gcggaggacc ggcgcgtcta
     1201 cgacctggtt tttctcgatc cgcccacgtt ttccaacagc aaacgcatgg acgacatcct
     1261 cgatgtgcag cgcgatcacg ccggcctgat cgacagttgc atggcgttgc tggcccccgg
     1321 cggaaagctg gtgttctcca ccaacgcgca gaaattcaag ctggacggca tgctcggcga
     1381 gcgttacaaa gtcacggaca tctcgcgcgc cacgctgccg aaggacttcg aacgcaatcc
     1441 gcgcattcat cagtgttacg aactggagtc aggaaaatga tcaagaaagg ttccgcggtc
     1501 tggaagggca ccctgaagga aggtaccggc agcatctcca ccgaaaccgg cgtgctgaaa
     1561 aacgcgccct atggtttcaa ggcgcgcttc gaagacgggc cgggcaccaa tccggaggag
     1621 ctcatcgccg ccgcgcacgc cgcctgttat tccatggcgc tgtcggcggg gctcggcaag
     1681 gaagggttca cggcagattc catcgaaacc agcgcggcgg tgcggctcga gaaagtcggc
     1741 gatggcttcg ccatcacgca cagcgatctc atttgtctcg cgaaggtgcc gaacatcacc
     1801 gaggcgaagt tcgcggaaat agccgaaatc acgaagtccg gctgcccggt atcgaaagtg
     1861 ctcaacgcca agatttccct ggtggcgaaa ctgacctagc tattcgtcgt catctgtgtc
     1921 gggtgcggac ggctgttcga agcggtaggc cagtaccttt ctgccgttcg ccggcacgcg
     1981 cagccgccag gcgaagtcgc cgtacttgcg gaaggcacgc tgactcgact tcgatatctt
     2041 cgcgcgcgtg tgcacatacg cgtaatggcg gatttcgaga gtcaccggca cgcccttggc
     2101 gttgagtgcg tgcaactcga catcggtgcg ttcgtcgaga ttcgcatcac gcaattcctc
     2161 tggatcgccc ggatcgacgt cgatcgtcag tgcg
//
LOCUS       DASQJS010000049         2910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3166422, whole genome shotgun sequence.
ACCESSION   DASQJS010000049 DASQJS010000000
VERSION     DASQJS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3166422"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VEW08_01910"
     CDS             complement(<1..925)
                     /locus_tag="VEW08_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ39512.1"
                     /translation="MKIRVFQSDKGDCLLLTSASGKNRVLVDGGMSSSYTKHVAPALA
                     RLSTNKSQLDVVYVSHIDDDHISGVLQLMNDLMDWRVFDFQTRHKKAGDKPPKKPKSA
                     RPPEVGKIWHNAFHELLKDNAGAIGEMLAATSAVLSASQRPMGKAIFEKHTELAFSKK
                     QALQLVRRTGADQLAIPVNPEFKHKLMLLLKTNPPKLKVGTMEFIIVGPAEADLSKLR
                     DEWNDWLRANKETLADIRRKAKADEDLLTSDVDRLLAPLTHQAGSFVDSQLELAKKLG
                     NRTEVTTPNLASLMFMVREGSQTLLLTGDGHA"
     gene            complement(922..>2910)
                     /locus_tag="VEW08_01915"
     CDS             complement(922..>2910)
                     /locus_tag="VEW08_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYJ39513.1"
                     /translation="GDANVNAIRAEMSAAVKVTNTRLLDKIPQIRGEGHEALSKADFR
                     AVFKRKASKPTHLKMLTGQVMTNVFIHTTTSDALPPKLVKESGRRGRIAKAMVSLDDL
                     PRLANDKRVRFVELGEPLKTPQPLVSTATVSAPATPRVKTTSAARHKGGKDVIIGIID
                     VQGFDFAHPDFLDAQGNTRFLSIWDQGGDARNPPDNAQFNYGAEFTQVHLNAAIKGAK
                     AARVPATEIERQSQREPESHGTHVASIAAGNRGICPNANIAAVLIDIPPEHMDRRKSF
                     YDSTRLADAVDYLVAFARRRRKPISINVSLGTNGHAHDMSSAINRWIDAVVNEPGVSI
                     AVAAGNSGQARADTQDPNDYGWLMGQIHASGRIPKAGLNVELEWVVVGNGVLDISENE
                     FELWYGPQDRFTVQLRPPGMDWLEEIEPGQFIENRQLPDGSFISIYNELYHSANGANT
                     ISIYLSPRLSQPAVIGVRAGTWLVRIRGKEVRDGGFHAWIERDDPQQIGPLGPKSMWR
                     FPSFFSDKTNVDESSVSSLACGERIVSVANLDESRRLINISSSQGPTRDGRSKPDVAA
                     PGTGIVAAKGFAGAKDLWVSMSGTSMACPYVAGVIGLMLAVQPNLTAAQIRGILLRTS
                     RPLPGNDYNWRKDAGFGVLDAEAAVQDALVINQRRDRT"
BASE COUNT          483 a          880 c          945 g          602 t
ORIGIN      
        1 cggcgtgtcc gtcaccggtg agcagcagcg tctgactacc ttcccgaacc atgaacatga
       61 gcgaggcgag attcggcgtg gtgacctccg tgcggtttcc caatttcttg gcgagctcga
      121 gctgcgaatc gacgaagctg ccagcctggt gcgtcagtgg agcgagcagg cgatcgacat
      181 cggaggtcaa caggtcttcg tccgccttgg ccttgcgccg gatgtccgcg agcgtttcct
      241 tgttggcgcg cagccagtca ttccactcgt cgcgcagctt gctgaggtcc gcttcggccg
      301 ggccgacgat gatgaattcc atcgtgccca ctttgagttt cggtggattg gtcttgagca
      361 acagcatcag tttgtgtttg aactcgggat tgacgggaat cgcgagctga tccgcgcccg
      421 tgcgccgcac gagctgcaat gcctgcttct tgctgaacgc gagttcagtg tgcttctcga
      481 agatggcttt gcccatgggc cgctggctgg ccgagagcac ggcactggtc gcggcaagca
      541 tctcgccgat cgcgcccgca ttgtccttga gcagctcgtg aaaggcgttg tgccagatct
      601 tgccgacttc gggcggccgc gcgctcttgg gctttttcgg aggcttgtca cccgccttct
      661 tgtgcctggt ctggaagtcg aagacgcgcc agtccatgag gtcgttcatc aattgcagca
      721 cgccggaaat gtggtcgtca tcgatgtgcg agacgtagac cacgtcgagt tgagacttgt
      781 tggtgctgag cctggcgagc gccggcgcga cgtgcttcgt gtacgaagaa ctcatgcctc
      841 catcgacgag cacgcgattc ttcccggaag ccgaagtgag cagcaggcag tcacccttgt
      901 ctgactggaa cacgcgaatt ttcatgtgcg gtccctgcgc tggttgatga ccaatgcatc
      961 ctggacggcg gcttcggcat cgagcacacc aaaacccgcg tccttgcgcc agttgtagtc
     1021 gttgccaggc agcggccgcg aagtgcgcaa caggattccg cggatctgcg ccgcggtgag
     1081 gttgggctgt accgcgagca tcagcccgat gacgcctgca acgtagggac aggccatgct
     1141 ggtgccggac atcgacaccc agagatcctt cgcacccgca aatcccttgg cggcgacgat
     1201 gccggttcca ggcgcggcga cgtcgggctt ggagcggccg tcgcgggtcg gcccctggct
     1261 actgctgatg ttgatcagcc ttcgcgattc atcgaggttg gcgacggaca cgatgcgctc
     1321 accgcaggcg agcgagctca ccgaggactc atcgacattg gtcttgtccg agaaaaacga
     1381 gggaaaacgc cacatgctct tgggccccag cgggccgatc tgctgcgggt cgtcgcgctc
     1441 gatccaggcg tgaaacccgc catcgcgcac ctccttgccc ctgatgcgaa ccagccaggt
     1501 gccggcgcgc acgccgatga cggcgggctg cgacaaccgc gggctcaggt agatagagat
     1561 ggtgttggcg ccgttggcgg agtgatagag ctcgttgtag atgctgatga agctgccatc
     1621 cggcagctgg cggttttcga tgaactggcc cggctcgatt tcctcgagcc agtccatgcc
     1681 cggcggacgc aactgcacgg tgaagcgatc ctgggggccg taccacagtt cgaattcgtt
     1741 ctcggagatg tcgagcactc cgttgccgac gacgacccac tcgagctcga cattgagacc
     1801 cgccttcgga atcctgccgc tggcgtggat ctggcccatc agccagccgt agtcgttcgg
     1861 atcctgcgta tccgcgcgtg cctggccgga gtttccagcg gcaacggcaa tgctgactcc
     1921 cggctcattg acgaccgcgt ctatccatcg attgatggcg ctgctcatgt catgcgcgtg
     1981 cccgttggtc ccgaggctca cattgataga gatgggtttc ctgcgccggc gggcgaacgc
     2041 caccaggtag tcgacggcgt ctgcgagacg cgtggagtcg tagaaggatt tgcggcgatc
     2101 catgtgctcg ggcggaatgt cgatgagcac cgcagcaatg ttcgcgttgg ggcagatgcc
     2161 acgattgccg gcggcaatgc tcgccacgtg tgttccgtgc gattccggtt cacgctgtga
     2221 ctggcgctcg atttcggtgg ccggcacgcg cgccgctttc gctcccttga tggccgcgtt
     2281 caggtgcacc tgggtgaact cggcgccata gttgaactgc gcgttgtcag gtggatttcg
     2341 ggcgtcgccg ccctggtccc agatcgacag gaaacgggtg ttgccctggg catcgaggaa
     2401 gtcgggatgc gcgaagtcga acccctgcac gtcgatgatg ccgatgatca cgtccttgcc
     2461 acccttgtga cgcgccgcgg aagtggtctt cacgcgcggc gtcgcaggag cgctgacggt
     2521 ggcagttgaa accaggggtt gcggcgtctt gagcggttcg ccgagctcca cgaaccgcac
     2581 ccgcttgtca tttgccagcc gcggcagatc gtcgagggag accatcgcct tggcgatgcg
     2641 gccgcggcgc ccggattcct tcacgagctt cggtggcagg gcgtccgagg tcgtggtgtg
     2701 aatgaacaca ttggtcatca cctggccggt gagcattttc aggtgcgtcg gcttggacgc
     2761 cttgcgtttg aacaccgcgc gaaaatcagc cttcgaaagc gcctcgtgac cttctccgcg
     2821 gatctgcggg atcttgtcca gcagccgcgt gttggtaacc tttaccgcgg cgctcatctc
     2881 ggcgcggatg gcattcacat tcgcatcgcc
//
LOCUS       DASQJS010000050         7360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1911156, whole genome shotgun sequence.
ACCESSION   DASQJS010000050 DASQJS010000000
VERSION     DASQJS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1911156"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VEW08_01920"
     CDS             <1..915
                     /locus_tag="VEW08_01920"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYJ39514.1"
                     /translation="RVGNISGVPQVAYGGQGGFGDVILHPRFAANSIVYVSYSEAGDG
                     STSGAAVARARLELNGDGGGSLQDLGVIWRQVPKVASGGHWGHRLAFGPDGMLWITSS
                     ERQQFTPAQDMKSNLGKVVRLKEDGSVPDDNPFYAQGGVTAQIWSLGHRNLLGIAFAP
                     DGRLYTHEMGPAGGDELNVIEKGANYGYPIVSNGNHYNGSDIPDHSTRPEFRAPLVTW
                     NPVISPAGFIIYSGKMFPDWTGNGFIGGLSSESLVRVEFGNGSAREAQRFDMGRRIRE
                     VEQGPDGAIWLLEDQRNLTGGRLLKLTP"
     gene            complement(905..2107)
                     /locus_tag="VEW08_01925"
     CDS             complement(905..2107)
                     /locus_tag="VEW08_01925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ39515.1"
                     /translation="MRRLTNKISLAMEERKRLRRRSPMSIAVADRFSQLSAEGWRAAT
                     DGGSVFHSAAYQRMFERVRPPNVEPRYALISDGDAPVAAVCLQIVTLDHSNLGDLRRR
                     RFLRKLGAKMRTRVLVCGNLLVYGLHGVSIAAGADRTKVWQAVSEVVYRVRRAEKLAG
                     STDIVVLKDFDEAARQDSAVLRKLSYGVVETEPNMVLTINPAWRRHEDYLQSLSSKFR
                     SDIKNRVFKKFDEAGCSVELLDDVAAHAPDLQQLYLQVHGNAGLRPFTVPASYWPELA
                     ELAGANARVHVARRNGRIVGFIVSVLDGETALAYHIGFDRQAAGEGVPVYLRLLHASL
                     ARAIESGARRVSFGRTALEPKARLGCKPEETIVWARHRHPILNQLLQPMLKLVEHDDA
                     PEVEPFKG"
     gene            complement(2108..3079)
                     /locus_tag="VEW08_01930"
     CDS             complement(2108..3079)
                     /locus_tag="VEW08_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39516.1"
                     /translation="MVLLAGLSWWLVNTPREHDAAVASSNQATSPDFPKTRLPGTIPG
                     KPTRITAARTNVEVEASTAASAPDWRKRLQDSDDYWAFAESAIGPARNGDGDARYWLS
                     LALNECVYVYSIYFFETLPDKPPRQLTVDEARQSVAQPSFYKPDDMDLLEKRCSRLSQ
                     SQGAPFGNGYDWMAAALAVDNPLAQANSAAEKALASRYIQDTEEAREARWEARRLIVD
                     ALRTRDPDVFLLVGNVAASLALDDEAEGKRRHLSWTLAACLRTPNCEGLSLWQKYRCN
                     WDSQCQPYETPRDIIQRDAGSDFDEVERRARELNEKIDAGAFDESDI"
     gene            complement(3185..3856)
                     /locus_tag="VEW08_01935"
     CDS             complement(3185..3856)
                     /locus_tag="VEW08_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HYJ39517.1"
                     /translation="MCGRYILAKQAKAETALGVQRLHWRDSPSYNVAPARDVPVVRIA
                     ADGGEREGVMMRWGLVPPFLKGEAPKFPTMNARIETMEASPAFRDAWKKGQRCIMPAE
                     GFYEWQMIPDAPRQPWFIGLANKEIMPFAALWERSVKADRTVVESCAIITLPANEFMA
                     KIHDEKRMPAILRMEDVEAWLTGTPQEARTTLLQYPGEQLRAHKVSVRVNSPKVDDER
                     LPEAI"
     gene            3936..4346
                     /locus_tag="VEW08_01940"
     CDS             3936..4346
                     /locus_tag="VEW08_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39518.1"
                     /translation="MKSFLAVLAGLVVTIVLSVGVDVLLVSTGLLPAKSADYTDTHWA
                     IITGYRVVIAIFGCWLAARLAPASPMNHALITGGIGLCIAIAGAIFAWNKGPAFGPHW
                     YAITLVITALPCAWIGGKINGDTPQLPAKGAAPP"
     gene            4343..5047
                     /locus_tag="VEW08_01945"
     CDS             4343..5047
                     /locus_tag="VEW08_01945"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HYJ39519.1"
                     /translation="MSDPRVADALAWLRKHSKKSIRDGMARYAIPSDKALGVLMGDIQ
                     KLAKLLGRDHALALELWKTDVYEARMLCAYVDEPERVTPALMDAQARDFDNWAICDTL
                     CFALWVRTPHAFAKIRKWANHKDEYVKRASFALLASMALKHKESTDADYLPLLPLMEK
                     AASDERNFVKKAVSWALRGVGRRSARLNKASLVLAKKLAASKDAAPRWIGKDAFRELS
                     SAAVQKRLKKVSGTVS"
     gene            complement(5067..5525)
                     /gene="aac(6')"
                     /locus_tag="VEW08_01950"
     CDS             complement(5067..5525)
                     /gene="aac(6')"
                     /locus_tag="VEW08_01950"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: protein motif:HMM:NF043067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047663 - aminoglycoside
                     6'-N-acetyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6'-N-acetyltransferase"
                     /protein_id="HYJ39520.1"
                     /translation="MPLKAATIERCSAINQPGWLQMRVALWPDATADEHRGYMAICLA
                     QPERFLQLMMYDEKRQPIGFIEGSIRSDYVNGTETSPVGFVEGVFVVPALRRHGVARR
                     LFEAIGDWAKARGCRELASDALLDNEASQRAHRALGFKETERVVYFSKTL"
     gene            complement(5889..6851)
                     /locus_tag="VEW08_01955"
     CDS             complement(5889..6851)
                     /locus_tag="VEW08_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39521.1"
                     /translation="MTNSGLRRGLFALVLGCFASLFFGGDAAAQAGASDTDESAYTPS
                     TLAKVMGGAARSQNEEAELGPPGQWYTTTATFTGQKRQPSSRFRPLISSWVSSRGQPA
                     DMVDMFFSGDLPELQFEQAGKKYWIMAGLPGGLGFDVYPTGQKLAIYLQRVGFASGTP
                     VAVLTLAKLPSGVTATTVPAGRRMHNTRMGEGARRQEATFKNGTATFTYAGREYSLPL
                     DVAGRHSLTPGLYSYENGVRTALLEYGAGDDQYLLLRVPVPDGKEAASLVPYLVLNGR
                     IVPPIDIGACAISVEKVQRAGAAGNVDCGDAPRAVFSKLRFTAE"
     gene            complement(6848..7150)
                     /locus_tag="VEW08_01960"
     CDS             complement(6848..7150)
                     /locus_tag="VEW08_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HYJ39522.1"
                     /translation="MNEPTRQEIDAAREPLVLEFGASWCGYCQAAQADIATVLKAQPA
                     VRHIKVEDGKGRPLGRSFGVKLWPTLIYLEDGVEKGRVVRPADSAAIEAIFAGAKS"
     gene            7174..>7360
                     /locus_tag="VEW08_01965"
     CDS             7174..>7360
                     /locus_tag="VEW08_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39523.1"
                     /translation="MDSPTYKFKVLSLDNDIMRGESVDSCGGLVGTNNIVLIHGLWMT
                     PLSWEFWAHHYTERGYSV"
BASE COUNT         1373 a         2357 c         2248 g         1382 t
ORIGIN      
        1 cgcgtcggca acatcagcgg agtgcctcag gtcgcctatg gcggacaggg cggcttcggt
       61 gacgtcatcc tgcatccgcg gttcgcggcc aactccatcg tctacgtgag ttattccgag
      121 gccggggacg gaagcacaag cggtgccgcg gtcgcgcgcg cccgtctcga gctcaatggt
      181 gatggcggtg gcagcctcca ggacctcggg gtgatctggc gccaggtgcc gaaagtggcc
      241 agcggcgggc actggggtca tcgcctggcg tttggacccg acggcatgtt gtggatcact
      301 tcgagcgagc gccagcagtt cacgcccgcg caggacatga agagcaacct cggcaaggtc
      361 gtgcgcctga aagaagacgg ctcggtgccc gacgacaacc cgttctacgc gcagggtggc
      421 gtcaccgcac agatctggtc gctcggccat cgcaacttgt taggtatcgc gttcgcgccc
      481 gacggcaggc tgtacacaca cgaaatggga ccggccggcg gcgacgagct caacgtcatc
      541 gagaaagggg ctaactacgg ctaccccatc gtctcgaacg gcaatcacta caacgggtcc
      601 gacatccccg atcactcgac gcgccccgag ttccgggcgc cgctcgtgac ctggaacccg
      661 gtgatatcgc ccgcggggtt catcatctat tccggaaaga tgttccccga ttggactgga
      721 aacggcttca tcggcgggct gtcatcggaa tcgctggtgc gcgtggagtt cggcaatggc
      781 agcgcgcgtg aagcgcagcg tttcgatatg ggccggcgca tccgcgaagt ggagcaaggc
      841 ccggacggcg ccatctggct gctcgaagac cagcgcaatc tcaccggcgg acggctcctc
      901 aaactcaccc cttgaagggt tcgacctcgg gcgcgtcgtc gtgctcgacc agcttcagca
      961 ttggctgcag caactggttc agaatcggat ggcgatggcg cgcccagacg atcgtctcct
     1021 cgggcttgca gcccagcctc gccttgggct cgagcgctgt ccggccgaac gacacgcgcc
     1081 gcgcgccgga ttcgatggca cgcgccagcg acgcatgcag cagccgcaga tacaccggca
     1141 caccctcgcc ggccgcctga cggtcgaagc cgatgtgata ggcgagcgcc gtttcgccat
     1201 ccagcacact gacgatgaac ccgacgatgc gcccattacg gcgcgccacg tgcacccgcg
     1261 cgttcgcgcc agcgagttcc gccagctccg gccagtagct ggccgggaca gtgaagggcc
     1321 tcaagcccgc gttgccgtgc acctgcagat acagctgctg cagatccggc gcatgtgccg
     1381 cgacatcgtc cagcaactcg acactgcaac ccgcttcatc gaacttcttg aaaacacgat
     1441 tcttgatgtc gctgcgaaac ttcgagctca ggctctgcag atagtcctcg tggcgtcgcc
     1501 aggcagggtt gatcgtgagc accatgttcg gctcggtttc caccacgccg taactgagtt
     1561 tcctgagcac cgcactatcc tggcgagcgg cttcgtcgaa gtccttgagc actacgatgt
     1621 ccgtgctgcc agcgagtttt tccgcgcggc gcacgcggta gacgacctcg ctcaccgcct
     1681 gccacacctt ggtgcgatcc gcgccggcgg caatgctcac gccgtgaagg ccgtaaacca
     1741 gcaggtttcc gcacaccagc acccgcgtgc gcatcttcgc gcccagcttg cgcagaaagc
     1801 ggcggcgcct caggtcgccc aggttcgagt ggtccagcgt cacgatctgc aggcagaccg
     1861 ccgccacggg cgcatcgcca tcgctgatca acgcgtaccg aggctccaca ttcggcggcc
     1921 gcactcgctc gaacatgcgc tggtaggccg cggaatggaa aacactgccc ccgtcggtcg
     1981 ccgcgcgcca gccttcagcc gacaactgcg agaagcggtc cgcgacggcg atggacatcg
     2041 gtgaccgccg gcgcagccgc ttgcgttctt ccatcgccag cgagatcttg ttggtcagtc
     2101 tgcgcattca gatatccgat tcgtcgaaag cacccgcatc tatcttctca ttcagctcac
     2161 gcgcgcgtct ctcgacttca tcgaaatcgc tgccggcatc acgctggatg atgtctcggg
     2221 gcgtttcgta cggctggcac tgcgaatccc agttgcaacg gtatttttgc cacaaggaga
     2281 gcccttcgca gttcggcgtg cgcaggcacg cggccagtgt ccacgataaa tggcgccgct
     2341 tgccttccgc ttcgtcatcg agcgcgaggg acgcggcaac attgccgacc aggagaaaga
     2401 catcgggatc ccgcgtgcgg agcgcatcga cgatcagcct cctggcttcc cagcgcgctt
     2461 cacgggcctc ttccgtatct tgaatgtacc tgctcgcaag cgccttctca gcggccgaat
     2521 tcgcctgcgc aagaggattg tcgaccgcga gcgctgccgc catccagtca tagccgttac
     2581 caaatggtgc gccctgggat tgactcagcc ggctgcaacg cttctccagc aggtccatgt
     2641 cgtcgggctt gtagaaggaa ggctgcgcta cgctttgcct ggcttcgtcg actgtcagtt
     2701 gcctgggagg cttgtccgga agggtttcga agaaatagat cgaatagaca tagacacatt
     2761 cgttgagcgc gagcgacagc cagtatcgcg cgtcaccatc gccgtttctt gccggcccga
     2821 tcgcgctttc cgcgaaggcc caatagtcgt cggaatcctg gagtcgcttg cgccagtcgg
     2881 gagctgatgc agccgtcgaa gcttcgacct cgacgtttgt cctggccgcg gttatccgtg
     2941 tcggcttacc tggaatcgtg cctggtaatc tggtcttggg gaaatcaggt gatgtggcct
     3001 gattggagct tgcgactgcc gcgtcatgct cgcgcggggt gttgactaac caccacgaca
     3061 gccctgcgag caacaccacg gccgcgaaac ccgaggatcg agggtttttc atcgcgatat
     3121 ccgccctctc acgccaaaac gactctccga tgtttcaacg gacgggccgc acgcacaggg
     3181 ttggtcagat cgcctcggga agcctctcgt catcgacctt cggcgaattc acgcgcacgc
     3241 tcactttgtg agcccgaagc tgctcgccgg gatactgaag caatgttgtc ctggcttcct
     3301 ggggtgtgcc cgtcagccac gcctccacat cttccatgcg cagaatggcc ggcatgcgct
     3361 tttcgtcatg gattttcgcc atgaattcgt tcgccggcag cgtgatgatg gcgcagcttt
     3421 ccaccaccgt ccggtcggct ttcacggacc tctcccacag cgcggcgaat ggcatgattt
     3481 ccttgttagc cagcccgatg aaccagggtt gcctgggcgc atcgggaatc atctgccatt
     3541 cgtaaaagcc ttccgccggc atgatgcagc gctgtccttt tttccacgcg tcgcgaaagg
     3601 ccggcgatgc ctccatggtt tcgatgcgcg cgttcatggt ggggaatttc ggcgcctcac
     3661 ctttgaggaa tggcggcacc agaccccagc gcatcatcac gccttcgcgc tcgccgccat
     3721 ccgcagcgat gcgcaccacc gggacatcgc gcgccggcgc gacgttgtaa ctcggcgaat
     3781 cgcgccagtg caggcgctgt acgccaagcg ccgtttcggc cttggcttgt ttggcaagaa
     3841 tgtagcgtcc acacataggg acagcttagc ggtcgcggct tgaccggagc aaacgagcgc
     3901 tccagcatgc gtcgaaaatc ctggggagca ggagaatgaa gagtttcctt gcggtacttg
     3961 caggtctggt agtcacgatc gtgctgtcgg tcggagtcga tgtgctgctg gtttccaccg
     4021 gcttgcttcc agcgaagagc gccgactata cggatacgca ctgggcaatt attacgggct
     4081 atcgcgttgt gatcgccatc ttcggttgct ggctggcggc tcggctcgca ccggcaagtc
     4141 cgatgaacca cgcgctgatc accggcggca tcggcttgtg catcgcaatt gcaggcgcca
     4201 tcttcgcctg gaacaagggt ccggcattcg gaccacactg gtatgcgata acgctcgtga
     4261 tcaccgctct gccctgtgca tggatcggtg ggaaaattaa tggggacact ccccaattgc
     4321 cagcaaaagg ggccgcacct ccatgagcga tccgcgcgtt gccgacgcac tcgcctggct
     4381 caggaaacac tcgaagaaat ccattcgcga tggcatggcg cgttacgcca ttccgagcga
     4441 caaggcgctc ggcgtgctca tgggcgacat ccagaagctg gccaaactgc tcggccgcga
     4501 ccacgcgctg gcgctcgagt tgtggaagac cgatgtctac gaggcgcgca tgttatgcgc
     4561 gtacgtggac gagcccgagc gcgtcacgcc ggcactgatg gatgcccagg cacgcgactt
     4621 cgacaactgg gccatctgcg acacgttgtg cttcgcgttg tgggtgcgaa caccgcacgc
     4681 gttcgcgaag atcaggaagt gggccaatca caaggatgag tacgtgaagc gcgcgtcatt
     4741 cgcattgctc gcgagcatgg cgttgaaaca caaggagtcg accgacgccg actacctgcc
     4801 cctgctgccg ctcatggaga aggctgccag cgacgaacgc aacttcgtga agaaggcggt
     4861 gagctgggca ttgcgcggag tcggacggcg aagcgccagg ctcaacaagg cttcgctggt
     4921 actcgcgaag aagttagccg cgtcgaagga tgcggcgccc agatggatcg gcaaggacgc
     4981 gttccgcgaa ctgtcgagcg ccgccgtgca gaagcgcctg aaaaaggtgt caggcaccgt
     5041 ttcctagaaa acggtgcctg accccattaa agggtcttgc tgaagtagac gacgcgctcg
     5101 gtctctttga atcccagggc gcggtgcgca cgctggctcg cctcattatc aagcagcgcg
     5161 tcggacgcca gttcccggca accgcgcgcc ttcgcccagt caccgatggc ttcgaacagc
     5221 cggcgcgcga ccccatggcg gcgaagcgca ggcaccacga agacgccctc gacgaaaccg
     5281 accggcgacg tttccgtgcc atttacatag tcgctgcgga tcgagccttc gatgaatccg
     5341 atcggctggc gcttctcgtc gtacatcatg agttgcagga aacgctctgg ctgggcgagg
     5401 catatcgcca tatagccgcg atgctcgtcg gcggtcgcat ccggccatag cgccacgcgc
     5461 atctgcagcc agccgggctg gttgattgcg ctgcagcgtt caatagtggc ggccttaagg
     5521 ggcatgcccg ggagggtatc aggaccgagt cgatttcata cagtgcccca cccacaccgg
     5581 tactgggtgg acgtgcggcg gccgtccgga aaaacttccg cccatgcccc ttcccgccag
     5641 cgccatcgac aaactctcag accactcagg gtcttacgtc atctgcgtct cctgccgcca
     5701 atgccagcac gagcgcgaaa tggatccgcg ctccctagct aacatccttg gctggaacgt
     5761 cacattgaag caggcctgca ctcgactccg ctgttcgaac tgccaggcac gtgctttcaa
     5821 agtggagatc gccttcgacc gcaaacctcg gggatggaaa gccaatccgt cctgatgaat
     5881 gttgccagct attccgcggt gaatcgcagc ttcgagaaca cggcgcgcgg cgcgtcgcca
     5941 cagtccacgt taccggccgc gccggcgcgc tgcactttct ccacgctgat tgcgcacgca
     6001 ccgatgtcga tgggtggcac gatgcgcccg ttgagcacga gatacggcac gagactagcg
     6061 gcttccttgc cgtccgggac cggaacgcgc agcaacaggt actgatcgtc gcccgcgccg
     6121 tattcgagca gcgcagtgcg cacaccgttt tcgtacgaat acagcccggg cgtcagggag
     6181 tggcgccccg ccacgtcgag cggcagtgaa tattcgcgac cggcgtacgt gaacgtggcc
     6241 gtgccattct tgaacgtggc ctcctgccga cgcgccccct cgcccatgcg cgtgttgtgc
     6301 atgcggcggc ccgcggggac cgtggtggcg gtgacgccgg agggaagttt cgccagcgtg
     6361 agcacggcga ccggcgtgcc ggacgcaaaa ccgacgcgct gcaggtagat agccagcttc
     6421 tggccagtcg gatacacatc gaaacccagc ccgcctggca agcccgccat gatccagtat
     6481 ttcttgccgg cctgttcgaa ctgcaactcg ggcaggtcac cactgaagaa catgtccacc
     6541 atatcagccg gctggccgcg gctgctgacc cacgagctga tgagcggacg gaatcggctc
     6601 gaaggctggc gcttctggcc ggtgaacgtg gccgtggtgg tgtaccactg ccctggaggc
     6661 ccgagttcag cctcttcgtt ttgcgagcgt gccgcgccac ccatgacctt ggcgagcgtg
     6721 gatggcgtgt acgctgattc gtcagtatcg ctcgcacccg cttgcgccgc ggcgtcgcca
     6781 ccgaagaaaa gcgatgcgaa acaacccagc accagcgcga ataacccgcg gcgcagtcca
     6841 gagttcgtca agatttcgct ccggcgaaga tcgcttcgat ggcggcacta tcggcgggtc
     6901 gcacgacgcg ccccttctcc acgccatcct ccaggtagat gagcgtgggc caaagcttca
     6961 caccgaatga ccgtccgagc ggccgaccct tgccatcttc caccttgatg tggcgcactg
     7021 caggttgtgc cttcaacacc gtggcgatat cggcctgcgc ggcctggcag taaccgcacc
     7081 agctggctcc gaattcgagt acgaggggct cgcgggcagc gtcgatttcc tgacgcgtcg
     7141 gttcgttcat tgcgcaatcc taggtgaaaa ttcatggatt cacctacgta taaattcaag
     7201 gtgctgtcgc tggacaacga catcatgcgg ggagaatctg tcgactcttg tggaggccta
     7261 gtgggcacta acaacatcgt cttgatccac gggttatgga tgacgccgct cagttgggag
     7321 ttttgggcgc accactacac ggaacgtgga tacagcgtgt
//
LOCUS       DASQJS010000051         6169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_968067, whole genome shotgun sequence.
ACCESSION   DASQJS010000051 DASQJS010000000
VERSION     DASQJS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968067"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="VEW08_01970"
     CDS             <1..1431
                     /locus_tag="VEW08_01970"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYJ39524.1"
                     /translation="GWPDQTVSLKQFYPTSVLVTGFDIIFFWVARMIMMGLKFQGDVP
                     FKDVYITGLIRDEHGDKMSKTKGNVIDPLDIIDGIALNDLVAKRTTGLMQPHLAAGIE
                     KNTRKQFPDGIAAFGTDALRFTFASLAGPSRDIRFELGRVGGYRNFCNKLWNGARFVL
                     MTVEGDAGQGDLAGAAELSVADRWIVSRFAATLAQVDSALTEYRFDYAATALYEFTWY
                     EFCDWYLELTKPVLQGEASTEAQKRGTRRTLVTVLEALLRALHPMMPFITEEIWQRVH
                     PLAAPLLAARKTRANQGTADSVMLAAYPAASDYARDTDAESQVSLLKEFILKVRQIRG
                     EMNIAPSRKIPLLVRDADEQAQLLIRTNAHYLHRLAGLESLKVLGSGDAEPVSASAMV
                     VATTLLVPLAGLIDAAAEIDRLTKVIGKNESDISKLRGKLGNESFVKNAKPELVAADR
                     VRLAELVAQNESMGKQLERVRRLGGS"
     gene            1476..2417
                     /locus_tag="VEW08_01975"
     CDS             1476..2417
                     /locus_tag="VEW08_01975"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYJ39525.1"
                     /translation="MNPPPAALNPALAQALAALDRVILGKSEAVRLALTCLLARGHLL
                     IEDVPGVGKTTLAHALAQVLGLAWQRVQFTSDLLPADIIGVSVFDAASGGFSFRKGPI
                     FTQLLLADEVNRASPKTQSALLEAMEERQVSVDVTTYRLPDPFFVVATQNPFEQLGTF
                     RLPESQLDRFLMRISLGYPDAANERALLAEGDRRELLSGMTPALNPDGVRELQAAAQR
                     VHVAPALLEYVQTLIASTRQRTDVLLGLSPRAGQGLVRAARAWAMLAGRDMVIPEDVQ
                     AVFAPVASHRLERAGVATALDHAAFIAELKRGVPVPV"
     gene            2509..3495
                     /locus_tag="VEW08_01980"
     CDS             2509..3495
                     /locus_tag="VEW08_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYJ39526.1"
                     /translation="MSQVIPVSRPGTVSRLLGAWARRRQGDDRLPVVISTRRVYILPT
                     RAGLAFAALVFMMLLAGLNYTNSVALMITFLLAGFGMIAAHLTHRNLTGVALRTVAGV
                     DAFVGEHGRLLVTLENSADTARLALACEVTGAPRAAVDVPAGGTARADVELSLTRRGR
                     LEVDRITLSTMFPFGLFRAWTYVHIKASLLAWPVPRGRRETPPEASSGGNAPAVHRVG
                     DEEWAGLREFRSGDSPRQVAWGAYARGRGLLVKTYQSPAAHHRLFDLAAVPGTDLEQR
                     LEQLSAWIVAAHARGERYGLRLADHTLLPDSGNEHRARCLNGLALFGTGETW"
     gene            3489..5450
                     /locus_tag="VEW08_01985"
     CDS             3489..5450
                     /locus_tag="VEW08_01985"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HYJ39527.1"
                     /translation="MVSAAARSTSAATLVFVASVLLIATDAPAWCVVIALAAAMWRVL
                     VALGHVAPVKRFAGMRFVLGAITAALVIAVAASFRTLNGLAAGTALLLVMGALKLLES
                     RSRRDDGIVIGVALFMLLASALATQSLIRVPFYLLTVWGACAAIAVVADRGGALTPKA
                     ALRLSARALAMSIPLAAACFLFFPRVTGQFWALQRGDEATTGLSGEMTPGGIGRLAID
                     YVPAFRVRFEGKPPPPPDLYWRGPVLNEFDGFTWRRSRGAYHPGARLEMVGAPIRYRV
                     TLEPSQQRWLFALDTVDTVSRRDVFIAFQDRQLSVIEPITSTVSYDAVSYLQTHSQGP
                     LANSGRRHETRLPPDRNPRARALALDLRARTGSDAEFARAALAWFRDNGLVYTLEPGV
                     TTIDSVDTTLFDSKQGFCGHFASAYATMMRAAGVPARIVTGYLGGEWNPVGGYLLVRQ
                     SDAHAWTEIWLEDTGWTRVDPTAVVAPGRLQRGIFDLLSESMSGRSSFRRSTWLIRLD
                     HLWDGANHWWQERVVDFNLRSQLDLLRKLGFDSPSWQQLGWAFTAGLVAWIAWVSLAL
                     RRGVARKKTDRIARAWMRATRKLARVAPARAPAEGPMEYARRVGAHRPDLAVRVDALA
                     SRYARLRFGRDASHHDLAAFEREVRNLAV"
     gene            complement(5431..>6169)
                     /locus_tag="VEW08_01990"
     CDS             complement(5431..>6169)
                     /locus_tag="VEW08_01990"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ39528.1"
                     /translation="LADASQAVRASFVTVAVWWLVFTIPLMRVVREQQRGPHLGFAEA
                     IRVGFRELGATIAHVRQYRTIAMFLLAYWFYIDGVNTIIKMAVNYGVSLGLDTNSLLT
                     ALLVTQFVGFPAALFFGYLGDRIGAKRGILIGLVVYAGITVYAYFLDSVGEFYALAVT
                     VGLVQGGVQSLSRSLFGRLVPEGKNAEFFGFYNMMGKFATVLGPLLIAIVAGLTHNER
                     ASIISLVLLFVVGGVLLWRVPITRQDS"
BASE COUNT         1028 a         2096 c         2004 g         1041 t
ORIGIN      
        1 ggctggccgg accagaccgt ttcactgaag cagttctacc cgaccagcgt gctggtcacg
       61 ggtttcgaca tcattttctt ctgggtcgcc aggatgatca tgatgggcct gaagttccag
      121 ggcgacgtgc cgttcaagga tgtgtacatc acgggtctca tccgcgacga acacggcgac
      181 aagatgtcga agaccaaggg caacgtcatc gacccgctgg atatcatcga cggcattgcg
      241 ttgaacgacc tcgtcgccaa gcgcaccacc ggcctcatgc agccgcatct cgccgccggc
      301 atcgagaaaa acacgcgcaa acagtttccc gacggcatcg ccgcgttcgg caccgatgcg
      361 ctgcgtttca cttttgcttc gctggccgga ccgtcgcgcg acattcgctt cgaactgggg
      421 cgcgtcggcg gctaccgcaa tttctgcaac aagctgtgga acggcgcgcg cttcgtgctc
      481 atgaccgtcg aaggtgacgc gggccaggga gacctcgccg gcgctgccga actctccgtg
      541 gcggatcgct ggatcgtctc gcgcttcgcg gccacgctgg cgcaggtaga tagcgcgctc
      601 accgagtatc gcttcgacta cgccgcgacc gcgctgtatg aattcacctg gtacgagttc
      661 tgcgactggt acctggagct gacgaaaccc gtcctgcagg gcgaggccag caccgaggcg
      721 cagaagcgtg gcacgcgccg cacgctggtg acggtgctcg aggcgctgct gcgcgcgctg
      781 catccgatga tgcctttcat caccgaagag atctggcagc gcgtgcatcc gctggcggcg
      841 ccgctgctcg ccgcgcggaa gacgcgcgcc aaccagggaa ccgcggacag cgtgatgctc
      901 gcggcctacc cggccgccag cgactacgcg cgcgacacgg acgccgagtc gcaggtgagc
      961 ctgctcaagg aattcatcct caaggtgcgc cagatccgcg gcgagatgaa cattgcgccc
     1021 tcgcgcaaga tcccgctgct ggtccgcgat gccgacgagc aggcgcagct gctcatccgc
     1081 accaatgcgc actatctaca tcgcctggcc ggcctcgaga gcctgaaggt tctcggctcc
     1141 ggtgatgccg agcccgtttc ggccagtgcc atggtggtgg ccaccaccct gctggtgccc
     1201 ctggcggggc tcatcgatgc cgcggccgag atcgaccggc tgaccaaggt catcgggaag
     1261 aacgaatccg acatcagcaa gttgcgcggc aagctgggca atgagagttt cgtgaagaat
     1321 gccaagccgg agctggtcgc ggccgaccgg gtgcgcctcg ccgaactcgt cgcgcagaac
     1381 gaaagcatgg gcaaacagct cgaacgggtg cggcgccttg gcggaagctg aagcgccatc
     1441 acgattaatc tgcggcccag ggatatagtc ggatcgtgaa cccgccacca gccgccctga
     1501 accccgcgct tgcccaggcc ctggccgcgc tcgaccgcgt catcctcggc aagtccgagg
     1561 ccgtgcgcct cgcgctcacc tgcctgctgg cacgcggcca cctgcttatc gaggacgtgc
     1621 ccggcgtcgg caagaccacg ctcgcgcacg cgttggcgca ggtgctgggc ctcgcctggc
     1681 aacgcgtgca attcaccagc gatctgctgc ccgccgacat catcggcgtc tcggttttcg
     1741 atgcggcgag cggcggcttc tctttccgga aaggtccgat attcacccag ctgctgctgg
     1801 ccgacgaagt gaaccgcgcc agccccaaga cgcaaagtgc gctgctcgag gcaatggaag
     1861 agcgccaggt cagtgtcgac gtcaccacct atcggctgcc ggacccgttt ttcgtggtcg
     1921 ccacgcaaaa tccgttcgag cagctcggca ccttccgcct tccggaatca cagctcgacc
     1981 ggttcctcat gcgaatctcg ctgggttatc ccgacgcggc caacgagcgg gcgctgctcg
     2041 ccgaaggcga tcgccgcgag cttctctccg gcatgacgcc cgcgctgaac ccggacggcg
     2101 tgcgcgaact gcaggccgcc gcgcaacgcg ttcacgtggc gccggcgctg ctcgaatatg
     2161 tgcaaacgct gatcgcgagc acgcgccagc gcaccgacgt gttgttgggc ctgagcccgc
     2221 gcgcgggcca gggcctggta cgcgccgcgc gcgcgtgggc catgctcgcg ggccgggaca
     2281 tggtgattcc cgaagacgtg caggcggtgt tcgcgcccgt cgcctcgcac cgcctcgaac
     2341 gcgcgggcgt ggctaccgcg ctcgatcacg ctgcattcat cgccgagctc aagcgcggcg
     2401 tgccggtacc ggtgtagata gcccgatgct ggtgccgccg ctaacggtgc caacgctgcc
     2461 gggccgaatt ggagtccggc aaatcgacgg agcggattcg gtggcgcgat gagccaagtt
     2521 atcccggttt cccggcccgg caccgtcagc cgcctcttgg gcgcctgggc gcgccggcgg
     2581 caaggcgacg accggttgcc ggtcgtcatc agcacacgtc gcgtctacat cctgccgacg
     2641 cgcgccggcc tcgcgttcgc ggcattggtg ttcatgatgc tgctcgccgg gctcaactac
     2701 acgaacagcg ttgcgctgat gatcaccttc ctgctcgccg gctttggaat gatcgcggcg
     2761 cacctcacgc accgcaacct gacgggcgtg gcgctgcgaa ccgtggccgg cgtcgacgcc
     2821 ttcgtgggcg agcatggccg gctgctcgtg accctggaga attccgcgga taccgcgcga
     2881 ctggcgctcg cgtgtgaagt caccggcgcg ccgcgcgcgg ccgtggacgt accagccggc
     2941 ggcacggctc gtgccgatgt ggaactttca ctcacacgcc gcggccgact cgaggtcgac
     3001 cgcatcacgc tgtcgacgat gtttccgttc ggcctgttcc gcgcctggac ctacgtacac
     3061 atcaaggcaa gcctgctcgc ctggccggtg ccgcgcggcc gtcgcgagac accacccgag
     3121 gcttcgagtg gcggcaatgc gccggccgtg catcgcgtcg gcgatgaaga atgggcaggc
     3181 ttgcgcgaat tccgcagcgg cgattcgccg cgccaggtgg cctggggcgc gtatgcgcgc
     3241 ggccgcggcc tgctcgtgaa gacctatcaa tctccggcgg ctcatcatcg cctgttcgat
     3301 ctcgccgcgg tgccgggcac cgatctcgag caacgcctcg aacaactgtc ggcgtggatc
     3361 gtcgccgccc atgcgcgtgg cgagcgctac ggcctgcgac tcgcggacca cacgctgctg
     3421 ccggacagcg gcaacgaaca tcgggcgcgc tgcctcaacg ggctcgcgct cttcggcacc
     3481 ggtgaaacat ggtaagcgcg gccgcgcgat ccacgtcggc cgccacgctg gtattcgtgg
     3541 ccagcgtgtt gctgatcgcc acggacgcgc ccgcgtggtg cgtggtcatc gcgctggccg
     3601 ccgccatgtg gcgcgtcctg gtggcgctcg gccacgtcgc cccagtgaag cggtttgcgg
     3661 gcatgcgctt cgtgctgggc gcgatcacgg cggcgctggt gatcgcggtg gcagcgagtt
     3721 tccggacgct gaatggactc gcggccggca ccgcgctcct gctggtgatg ggcgcactga
     3781 agttgctgga gtcccgctcc cgccgcgacg atggcatcgt gatcggcgtc gcactgttca
     3841 tgctgctcgc ctcggcgctc gccacgcagt cgctgatccg cgttcccttc tatctactga
     3901 ccgtgtgggg cgcctgtgcg gcgatcgcgg tcgtcgcaga tcgcggcggc gcactgacac
     3961 cgaaggcggc gctgcgcctg tcggcgcgcg cgctggcgat gtccattccg ctggccgcgg
     4021 cgtgtttcct gtttttcccg cgggtcaccg gccagttctg ggccctgcaa cgcggcgatg
     4081 aagcaaccac cggcctgtcc ggcgaaatga cgccgggcgg catcggcaga ctggcgatcg
     4141 attacgttcc tgcgttccgt gtccggttcg aaggcaagcc gccaccaccg cccgatctgt
     4201 actggcgcgg tccggtgctc aacgaattcg atggattcac ctggcgcaga agccgcggtg
     4261 cgtaccatcc cggtgcgcgc ctcgagatgg tgggcgcacc gatcaggtat cgcgtcactc
     4321 tcgagcccag ccagcagaga tggttgttcg cgctcgacac cgtcgatacg gtttcgcgcc
     4381 gtgacgtctt catcgcgttt caagaccggc aactgtcggt gatcgaaccg attaccagta
     4441 cggtcagcta cgacgcggtt tcctacctgc agacacacag ccaggggccg ctcgcgaatt
     4501 caggccggcg tcacgaaacc aggttaccgc ccgatcgcaa tccgcgggct cgtgcactgg
     4561 cgctcgatct tcgcgcacgc accggcagcg acgccgaatt cgcacgcgcg gcgctcgcct
     4621 ggtttcgcga caatggcctc gtatacacgc tcgaacctgg cgtcaccacg atcgactcgg
     4681 tcgataccac tctgttcgac agcaagcagg gcttctgcgg ccactttgcg tccgcgtacg
     4741 ccaccatgat gcgcgcggcg ggagttccgg cacgcatcgt caccggttac ctcggtggcg
     4801 aatggaatcc cgtcggcggc tatctcctcg tgcggcaatc ggatgcgcac gcctggacgg
     4861 agatctggct ggaggacacg ggctggacgc gcgtcgaccc cacggctgtc gtcgcgccag
     4921 ggcgcctgca gcgcggcata ttcgacctgc tgtccgaatc aatgtcgggc aggagcagtt
     4981 tcaggcgcag cacatggctc attcgcctcg accatctgtg ggacggcgcc aatcactggt
     5041 ggcaggagcg cgtcgtggat ttcaacttgc gctcgcagct cgatctgctg cgcaaactcg
     5101 gtttcgattc gccgtcatgg cagcaactgg gatgggcatt cactgccggc ctcgttgcct
     5161 ggatcgcgtg ggtcagcctg gcgttgcgcc ggggagtggc gcgcaagaag acggaccgga
     5221 tagcacgtgc ctggatgcgt gccacacgca agctggcgcg tgtcgcgccg gcgcgggcgc
     5281 cggccgaagg cccgatggaa tacgcgcgca gagtcggcgc gcatcgcccc gatctggcag
     5341 ttcgcgtcga tgcactcgcc tcgcgttatg cccgcctgcg cttcggacgt gacgccagcc
     5401 accacgactt ggccgcattc gaacgcgagg tcaggaatct tgccgtgtga tgggaacgcg
     5461 ccacaacagc acgccgccaa ctacgaacaa cagcaccagc gagatgatcg aggcgcgctc
     5521 gttgtgcgtg aggccggcaa caatcgcgat gagcagtggt ccgagcacgg tcgcgaattt
     5581 gcccatcatg ttgtagaaac cgaaaaactc cgcgttcttg ccctccggca ccaggcggcc
     5641 gaacagcgaa cgcgacaggc tctgcacgcc gccctgcacg aggcccacgg ttaccgccag
     5701 cgcataaaac tcgcccaccg aatccaggaa gtacgcgtac accgtgatac cggcgtacac
     5761 cacgaggccg atgagaatgc cgcgtttcgc cccgatgcga tcgccgagat agccaaagaa
     5821 cagcgccgcc gggaatccca cgaactgcgt caccagcaac gccgtgagca gactgttggt
     5881 gtcgagcccg agtgaaacgc cgtagttcac cgccatcttg atgatggtgt tgacgccgtc
     5941 gatgtagaac cagtacgcca gcaggaacat cgcgatggtg cggtactggc gcacgtgggc
     6001 gatggtcgct ccgagttcgc ggaatcccac gcggatcgcc tccgcgaacc cgaggtgcgg
     6061 accgcgttgc tgctcccgca ccactcgcat cagcggaatc gtgaacacca gccaccacac
     6121 cgcaacggtg acgaaggatg cgcgcacggc ctgcgacgca tccgcgagg
//
LOCUS       DASQJS010000052         5907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_341493, whole genome shotgun sequence.
ACCESSION   DASQJS010000052 DASQJS010000000
VERSION     DASQJS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341493"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1755
                     /locus_tag="VEW08_01995"
     CDS             <1..1755
                     /locus_tag="VEW08_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ39529.1"
                     /translation="HGTDLATSVDGIPVNMHTHGHGQGYTDINFVIPELLDRIEYKKG
                     TYYADEGNFSAAGAVDLKYRRRLDDTMLSLTVGEDSYARALLAGSANLGGGDLLFGLD
                     YGVTDGPWDLPEDLSRVNALVKYSHGEPGDGYALTVSGYDGSWDSTDQIPERAVDSGL
                     LDRFGYVDGTDGGESHRYSFNFEIAKPLGEWNLEANAYALDYQLDLFSNFTYALDAAD
                     GDQFEQFDDRQAYGVHAEVRRANRIGNLPGQFRAGIESRFDDIGKVGLYLTEGRMRRS
                     TVREDAVQRLGFGFFIEQQLEATDWLRVIAGLRHDLADFDVDSDLDANSGSASDSITS
                     PKLSFVFGPWADTEFFINAGRGFHENDARGTTITVDPADGLTTADRVNPMVRASGAEV
                     GVRTAILPYTQLALTWWKLDLDSELLYVGDAGATEANRASDRHGVELGVYVTPREWLT
                     LDADLAWSHARFSESDPAGNYIPGAVESVASVGVALQHPSGWFGGARYRHFGSAPLIE
                     DDSARSEPTTVVNLELGHRFKRFSLSLAAYNLFDSHDNDITYFYESQLPGEGAPVEDR
                     HFHPVEPRTLRLTVETRF"
     gene            1800..3128
                     /locus_tag="VEW08_02000"
     CDS             1800..3128
                     /locus_tag="VEW08_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HYJ39530.1"
                     /translation="MLALSSRALNLAAALVAACLLAPVSAAQAPPKPATTTKKPATTS
                     SKPKPKPGTPAAKKPAKPVAPPPPPPPPPVELIGLIVMPADAMRAGPPSGAFDGEGRR
                     AAAPRFDAQPVQGVSSIKPGTTAGAWWALSDNGFGGKWNSSDYRLCIYLFDVRPRTEP
                     GGDSRNALQAVIELSDPAKFFPWRLAEEGSPERLLTGLDADPESLVTMPDESFWIGDE
                     MGPWLLHFSIDGELLAPPVELPDNVRSADHPLAIARADIPRLPHSRGIEAMDLAGDNK
                     TLVSILEGTVGGDTPRTLRVQRYDTSAGKWLPGTLIYERDTDTTSVTDLSLVEGNRFV
                     VIERDDQQGDAARAKRVYSIDLDRALPGKPLQKKLVIDLLTIGNARGLALTAPGAPFR
                     FPYLTTESIQVLDRKHVVIVNDNNYPAMGGRGASVKDATEWIWLELANPL"
     gene            3130..4944
                     /locus_tag="VEW08_02005"
     CDS             3130..4944
                     /locus_tag="VEW08_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ39531.1"
                     /translation="MTLIALKNAELAFGLHPLLDGASIAIQAGDRVGLIGRNGTGKSS
                     LLSALLGKIPLDAGEVQRRPGLSIASVEQEPVLPPAGTLRESLVLLGDFANMHDDRAL
                     WAREARLNEFLQRFSVNPDSSPASVSGGERKRAALALAFAMNPDLMLLDEPTNHLDVD
                     GIETLEGLLQRNVTCIVITHDRHFLDQVANSIAELDRGILRTFPGNYSVYEERKAEID
                     IAEDLAHRRFEKFWAQEEVWIRKGVEARRTRNEGRVKRLEHLREERAARRERIGQIKL
                     TVDTGERSGKLVAELENVGKSFGGRAILDDVSLRVMRGDRVGLLGPNGAGKTTMIKLI
                     VGTLEPDSGRVRRGTNLQVAYFDQLRDQLDPERTLAETVNPGSDWVQIGENRKHISSY
                     LQEFLFDSRRANAPIRMLSGGERNRLLLARLFARPANMLVLDEPTNDLDIDSLELLET
                     MLVDYTGTLLLVSHDRTFLDNVVTQTIASEGKGRWGEYVGGYSDWLRQRSLPRAAYGA
                     PRGVAAPPAAPAAPKRKLSFKEQRELDGLPGEIEKLEGEQLALTQKICEPDYHRAGAE
                     QMRVDRERASELATLIAARMERWEALEALASQVAGGKS"
     gene            4941..5207
                     /locus_tag="VEW08_02010"
     CDS             4941..5207
                     /locus_tag="VEW08_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="involved in start site selection during the
                     initiation of translation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HYJ39532.1"
                     /translation="MKPGTTNKPPAIRIWKETAHRGGKGVSAIHGIPLPGPALEEICS
                     QLKKKCGTGGTVKNGVIEIQGDHRDRLVEELRKLGYSDVKKAGG"
     gene            5312..>5907
                     /locus_tag="VEW08_02015"
     CDS             5312..>5907
                     /locus_tag="VEW08_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HYJ39533.1"
                     /translation="MRKNRLRPLVLLMLLPCAQVFATEPLQTSTKRLDVRAPAQIIRD
                     EDGVSHIFASTERDLLFLQGYAQARDRLFQMDTLRRQADGSLAELLGASVLPSDIQLR
                     TIGIRRAAEATLPALSAETRAGFKAYAAGVNAYTASHPLPPEYQRLEITRFRPWTEAD
                     SVSVLKLITFQLSFELSELQATVALRSYQAAGAQRGFDG"
BASE COUNT         1085 a         2006 c         1839 g          977 t
ORIGIN      
        1 cacggcacgg acctcgccac cagcgtcgac ggcatccccg tgaacatgca cacgcacggg
       61 cacggccagg gctacaccga catcaacttc gtgattcccg agctgctcga ccgcatcgag
      121 tacaagaaag gcacgtacta cgctgacgaa ggcaacttct ccgccgccgg cgccgtcgac
      181 ctcaaatatc gccgcaggct cgacgacacg atgctgtcgc tcaccgtggg tgaagactcc
      241 tacgctcgcg ccctgctcgc cggctctgcc aatctcggcg gcggcgacct gctcttcggt
      301 ctcgactacg gcgtcaccga tggaccctgg gatcttcccg aagacctgag ccgcgtgaat
      361 gcgctggtga agtattcgca cggcgaaccc ggcgacggct atgcactgac cgtatccggg
      421 tacgacggca gttgggattc cacggaccag attcccgagc gagccgtgga ctcgggcttg
      481 ttagaccgct tcggctacgt cgatggcacc gacggcggcg agtcgcatcg ctactcgttc
      541 aatttcgaga ttgcgaaacc gctgggtgaa tggaacctcg aggccaacgc ctatgcactc
      601 gactaccaac tcgatctgtt ctcgaatttc acgtacgccc tggacgctgc ggacggcgat
      661 caattcgaac agttcgacga tcgccaggcg tatggcgtgc acgccgaagt ccgccgtgca
      721 aaccgcatcg gcaacctgcc cggccagttc cgcgcaggaa tcgaatcccg cttcgacgac
      781 atcggcaagg tgggcctcta tctcaccgaa ggccgcatgc gccggtcgac cgtccgcgag
      841 gatgccgtgc aacgcctggg attcggcttt ttcatcgagc agcagctgga agccacggac
      901 tggctgcgtg tcattgcagg cctgcgccat gatctcgccg acttcgacgt cgacagcgac
      961 ctcgacgcga acagcggttc cgccagcgac agcatcacca gccccaaact ctcgttcgta
     1021 ttcgggccgt gggccgacac ggaattcttc atcaatgccg gacgcggttt ccacgagaac
     1081 gacgcgcgtg gcaccacgat taccgtggat cccgcggacg gcttgaccac cgctgatcgc
     1141 gtcaacccga tggtgcgcgc cagcggcgcg gaagtgggcg tgcgcaccgc aatcctgccg
     1201 tacacccagc tcgcgttgac ctggtggaaa ctggatctcg attccgaact gctctacgtg
     1261 ggtgatgccg gcgctaccga agcgaatcgc gccagcgatc gccatggcgt tgagctcggc
     1321 gtgtacgtga cgccgcgcga atggctcacg ctcgatgccg atctcgcctg gtcgcacgcg
     1381 cgcttcagcg aatccgaccc cgccggaaat tacattcccg gcgccgtgga aagcgtggcc
     1441 tcggtaggcg ttgcccttca gcacccgagc gggtggtttg gcggcgcgcg ctaccggcac
     1501 tttggcagcg cgccgctcat cgaagacgac agcgcgcgtt ccgagcccac gacggtggtc
     1561 aatctcgaac tgggacaccg tttcaaacgt ttctcgctct cgctcgcggc ctacaacctg
     1621 ttcgattcgc acgacaacga catcacatat ttctatgaga gccagttgcc cggcgaaggt
     1681 gcaccggtcg aagaccgcca tttccacccg gtggagccgc gcacactgcg cctgacggtc
     1741 gaaacacgtt tctaaggttg tgagcgcggg atgccacgcc acgcgtagaa tccgcggcca
     1801 tgcttgctct gtcttcgcgc gccttgaacc tggccgccgc cctggtcgcg gcgtgcctgc
     1861 ttgcgccggt atccgcggcc caggcgccgc cgaaaccggc aaccactacc aagaagcccg
     1921 ccaccactag ttcgaaaccc aagccgaagc ccggtacccc cgcggcgaag aagcctgcga
     1981 aacccgttgc accgccaccg cccccaccgc ctccgcccgt ggaactcatc gggctcatcg
     2041 tcatgcccgc ggacgccatg cgcgcgggtc cgccttccgg cgcattcgat ggtgaaggcc
     2101 gccgcgcggc cgcaccgcgc ttcgacgccc agcccgtaca gggcgtgagc tccatcaaac
     2161 ccggcaccac cgccggcgcc tggtgggcgc tctcggacaa tggcttcggc ggcaagtgga
     2221 acagttccga ctaccggctc tgcatctatc tattcgacgt acggccgcgc accgagcccg
     2281 gtggtgactc gcgcaatgcg cttcaggccg tcatcgaact atccgatccg gcgaagttct
     2341 ttccatggcg cctcgcggaa gaaggctcgc ccgagcggct gctcacgggt ctcgacgccg
     2401 atccggagtc gctggtgacg atgccggacg agtcattctg gatcggcgac gagatgggcc
     2461 cctggctgct gcatttctcg atcgacggcg agttgctggc gccgccggtg gaactgccgg
     2521 acaacgtgcg ctcggcggat catccgctgg ccatcgcccg cgctgatatc ccgcggcttc
     2581 cgcattcacg cggcatcgag gccatggacc tcgccggcga caacaagacg ctggtgtcca
     2641 ttctcgaagg tacggtgggc ggtgacacgc caagaacgct gcgcgtgcag cgctacgaca
     2701 cgtctgccgg aaagtggctg cccggcactc ttatatatga gcgggacacc gacacgacct
     2761 cggtcaccga cctgtccctt gttgaaggca accggttcgt ggtcatcgag cgcgacgacc
     2821 agcagggcga cgccgcccgc gccaagcgcg tgtattccat cgatctggac cgtgcgctgc
     2881 ccggcaagcc cttgcagaag aagctggtca tcgacctgct gaccatcggc aatgcgcgcg
     2941 gcctggcctt gacggcaccg ggcgcgccat ttcgctttcc gtatctgacg accgagtcca
     3001 tccaggtgct ggaccgcaag cacgtcgtca tcgtgaacga caataactac cccgccatgg
     3061 gtggccgcgg cgcgtctgtc aaagacgcca cggaatggat ctggctcgaa ctggccaatc
     3121 ccctgtaacg tgacactgat cgccctcaag aacgcggaac tcgcgttcgg cctgcatccg
     3181 ctgctcgatg gcgcttcgat cgccatacag gccggtgacc gggtcggact catcggccga
     3241 aacggtaccg gcaagtcctc cttgctgagc gcattgctcg gaaagattcc gctcgatgcc
     3301 ggcgaagtac agcgccggcc gggcctctcc atcgccagtg tcgagcagga gccggtgctg
     3361 ccgcccgccg ggacgttgcg cgagtcgctg gtactgctcg gcgatttcgc gaacatgcac
     3421 gacgaccgcg cgctgtgggc gcgcgaagcg cggctcaacg aattcctgca acggttctcg
     3481 gtcaatcccg attcatcgcc ggcttcggtt tccggtggcg agcgcaagcg cgccgccctc
     3541 gcgctggcgt tcgcgatgaa tcccgacctg atgctgctcg atgagcccac caaccatctc
     3601 gacgtcgacg gcatcgagac actggaaggg ttgctgcagc ggaatgtcac ctgcatcgtc
     3661 atcacgcacg accggcactt cctcgaccag gtggcgaatt ccatcgccga gctcgatcgc
     3721 ggcatcctgc gcacttttcc gggcaactac tcggtgtacg aggaacgcaa ggccgagata
     3781 gacatcgccg aagaccttgc gcaccggcgc ttcgagaaat tctgggcgca ggaagaagtg
     3841 tggatccgca agggtgtcga agcgcggcgc acgcgcaacg agggccgggt gaagcgcctg
     3901 gaacatctgc gcgaggagcg cgccgccagg cgcgagcgca tcgggcagat caagctcacc
     3961 gtggataccg gcgagcgctc cggcaagctg gtagccgagc tcgagaacgt cggcaagagt
     4021 ttcggcggcc gcgcgattct cgacgacgta agcttgcgcg tgatgcgtgg cgaccgcgtg
     4081 ggcttgttag gccccaacgg cgcgggcaag accaccatga tcaagctcat cgtcggcacg
     4141 ctggagccgg acagcgggcg cgtgcgccgc ggcaccaacc tgcaggtcgc ctacttcgat
     4201 cagctgcgcg atcagctgga cccagagcgc acgctggccg aaaccgtgaa ccctggctcc
     4261 gactgggtgc agataggcga gaaccgcaag cacatctcca gctacctgca ggaattcctg
     4321 ttcgacagcc gccgcgcgaa cgcgccgatc cgcatgctgt cgggcggcga gcgcaaccgg
     4381 ctgctgctgg cgcgcctgtt cgcgcgcccg gccaacatgc tggtgctcga cgagcccacc
     4441 aacgatctcg acatcgattc gctcgagctg ctcgaaacca tgctggtgga ctacaccggc
     4501 acgctgctgc tggtcagcca cgaccgcacg tttctcgaca acgtggtcac gcagaccatc
     4561 gcctccgaag gcaaaggacg atggggcgaa tacgtcggcg gctacagcga ctggctgcgg
     4621 cagcgatcgc tgccacgggc cgcctacggc gcgcctcgcg gcgtcgccgc accaccggcc
     4681 gctccagccg ctcccaagcg caagctcagc ttcaaggagc aacgtgagct cgacgggctg
     4741 cccggtgaga tcgaaaagct cgaaggcgag caactcgctc tgacgcagaa gatctgcgag
     4801 ccggattatc accgcgccgg cgccgagcag atgcgcgtcg atcgcgagcg cgcgagcgag
     4861 ctcgcgacgc tgattgcggc gcgcatggaa cgctgggagg cgctcgaagc gctggcatcg
     4921 caggtggcgg gcggcaagtc atgaagcccg gcacgaccaa caagccgccc gccatccgca
     4981 tctggaaaga aaccgcgcat cgcggcggca agggcgtgag cgccatccac gggattccgt
     5041 tgcccggccc ggcgctcgaa gagatctgca gccagctcaa gaagaagtgc ggcaccggcg
     5101 gcacggtcaa gaacggggtc atcgagatcc agggcgatca ccgcgaccgg ctggtcgagg
     5161 aactcaggaa gctcggctac agcgacgtga agaaggccgg cggctgaccg ccaatgcatc
     5221 cgccgccgct ttcatgacgt agttaggaat gagtggccgc cgtttccctc ggcgcaccac
     5281 caatgcccac tatcttgtga aggagtccgt catgcggaag aatcgtctgc gcccactcgt
     5341 gctgttgatg ttgctgcctt gcgcacaggt attcgccacc gagcctttgc aaacatccac
     5401 aaagcggctc gacgtccgcg cccccgcaca gatcatccgc gacgaagacg gtgtatcgca
     5461 tatcttcgca tcgaccgaac gcgacctgct gttccttcag ggctacgcgc aagcgcgcga
     5521 ccggttgttc cagatggata cgcttcgccg ccaggccgac ggcagtctgg cggagctcct
     5581 cggtgcatcg gtgttgccca gcgacatcca gctgcgcacg atcggcatcc ggcgagccgc
     5641 ggaggcaacg ctgccggcgt tgtcggcgga gacgcgtgcg ggcttcaagg cctacgccgc
     5701 gggcgtgaac gcgtataccg cttcgcatcc gttgccgcca gagtatcagc gcctcgaaat
     5761 cacgcgcttt cgcccgtgga ccgaggccga ttcagtgtcc gtcctcaagc tgatcacctt
     5821 tcagctgtcg ttcgagttga gcgagttgca ggcgacggtc gcgctgcgct cgtaccaggc
     5881 ggcgggtgcg cagcgcggct tcgatgg
//
LOCUS       DASQJS010000053        11966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_970841, whole genome shotgun sequence.
ACCESSION   DASQJS010000053 DASQJS010000000
VERSION     DASQJS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11966
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970841"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VEW08_02020"
     CDS             <1..1128
                     /locus_tag="VEW08_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HYJ39534.1"
                     /translation="RQYQVNPLHLYEERRPKPFKDPPEGAKSNRRPLTHYYLRIRTKG
                     GVEGIYGMVDKEALPVLLGTLRPLVIGQNALAVERIWDQMYRSNRHSRASHYMMAISY
                     MDNALWDLRGRHFGAPVFRLLGGPTQLPVRVYGSCLGFSIDPPLAGKRAAQLKKDGFI
                     HQKWFLGFGPGDGARGMDLNVQLVKALREAVGDDVQIMFDAYQGWDLQYAIEWCRKVE
                     QYRPYFVEEAVPMSDLESFIRLSKATTIPVATGEHLYGRWEAEQFFKTDALQFIQADP
                     EWCGGVSETVKVAHLASVHGVKLLPHCHNVHAALHIVASQSPSVCPFGEYLINHVPEK
                     LHFMKDPLLTTNGLVQLSEKPGFGIELDPAKVEKQEILVTL"
     gene            1154..2593
                     /locus_tag="VEW08_02025"
     CDS             1154..2593
                     /locus_tag="VEW08_02025"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYJ39535.1"
                     /translation="MRSFAAVVLSLGSLVTAHAADITASRQTAASKQARAGEAEFRAL
                     YRELVEINTTLSVGSCTEASNAMKARLSAAGYADEQLQVIVPPGRPKDGNLIAVLPGS
                     DQSLKPILLLAHIDVVEANRADWERDPFRLVEEGGFFYGRGSSDDKAMAAVFADSMVR
                     FKQEKYRPKRTIKLALTCGEETPNTFNGVSYLIEHHRDLIDAGFALNEGGGGRYDQKT
                     GVYRYVAVLASEKVYQDFTLTTHNQGGHSSRPTPDNAIYQLAHALDKIEAHQFKVEFN
                     ETSRAYFEKFGVIEGGEKGADMIAAAKNGDAAAIERLKKDPSSNAILRTNCVATQLAG
                     GHAPNALPQRATANVNCRMFPGRSAEEVRQELIAAIADPGVSVEFQATPEKPGAGPAL
                     SREVMGPIEALARDMYPGVAVIPTISSGATDGRFLTPAGIPTYGVSGMLGDGATGNAH
                     GLNERIRVQTLIEGREFLYRLTKAYAGGK"
     gene            complement(2612..3772)
                     /locus_tag="VEW08_02030"
     CDS             complement(2612..3772)
                     /locus_tag="VEW08_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HYJ39536.1"
                     /translation="MNKFHVRAALLAASGFLAVCALAKDAAPAADPKAVELIREHGLA
                     ESPSALRDIRGWRAPRRIVMATQNPTGTSTQEIRATLEGVAPGIEMVFVADAAGLIKE
                     AADADAIIGGDDIACDDRVLAAAKRVRWIAVMSAGVEACLGKPALERPGLIVTNMRAV
                     AGPVMAEHSIALMFALSRSLQVSVGRQATGEGWNRNFTGSQPQSLTGKTLLVAGLGGI
                     GLEVAKRGHALGMKVIGTRNTSREGPDYVSRVGLSDELPAMIGEADVVVAALPLVPAT
                     TRLFDAKMFARMKKTAYFISVGRGGSVVTDDLVAALNAGTIAGAGLDVTDPEPLPKDH
                     PLWKARNIIITPHMSALSDLGQSARMLLLKEQVRRFAAGDKLVAVVDFRKGY"
     gene            complement(3915..6383)
                     /locus_tag="VEW08_02035"
     CDS             complement(3915..6383)
                     /locus_tag="VEW08_02035"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HYJ39537.1"
                     /translation="MSKEALKHAFLDNLFYIQGKFPALATKTDYYMALAYAVRDRMLQ
                     RWISTAAAYTKQGSRTVAYLSAEFLMGPHLGNNLINLGIYETARTAVAELGLDLEELL
                     AHEDEPGLGNGGLGRLAACFIDSLATLEVPALGYGIRYEFGIFQQEIVDGWQVEKTDK
                     WLRYGNPWEIARPEWCCDVKFGGHTEVWYDEYGRKRTRWVPGHIVNGIPYDTPILGYH
                     VNTANTLRLWKAEAPESFDFGTFNRGDYYGAVNQKVASENLTKVLYPNDEQARGKELR
                     LEQQYFFVSCSLQDMLRICRVQKIPPERFHEKFAIQMNDTHPSIAVAELMRLLVDEMA
                     MDWDRAWDITRKTCSYTNHTLLPEALERWPLPMFGRLLPRILEIIYEINARHLDEVRM
                     AFFGDEQIISRMSLIDEAGERYVRMANLASVGSHAINGVAELHSELLKRDVLKDFALL
                     WPEKFSNKTNGVTPRRWMVLSNPRLAHCITQRIGDGWIRDLYELRKLEPLVDDPDFRA
                     EWRQIKHANKTALAGLIEQRTGVIVDPSAIFDVQVKRIHEYKRQHLNILHVIGLYHRL
                     KTDPKFTMEPRVFVFGGKAAPGYFLAKLIIRLITAAAEIINRDPGVRDLIKVVYLPNF
                     NVTNGQKIYPAADLSEQVSTAGKEASGTGNMKFQMNGALTIGTVDGANIEICEEVGVE
                     NFFLFGLEAHEVAARRAAGYRPMDFVENSEELQAILQLIRDGFFSRGNSSQFQPLLDN
                     LVHHDPYFVFADYQSYADCQTLVDQTYRDKEGWTRMSILNSARSGKFSSDRTIREYCN
                     EIWKAKAVPVQLLSQEEVKAGLLQ"
     gene            complement(6643..8064)
                     /locus_tag="VEW08_02040"
     CDS             complement(6643..8064)
                     /locus_tag="VEW08_02040"
                     /inference="COORDINATES: protein motif:HMM:NF027805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HYJ39538.1"
                     /translation="MKRRSNGAAALMAAAFACVAGIASADVVKKGSDVYLITLDPGHF
                     HAALVQKFMLPRVSPDVRVFAPAGDDVAQHLKRIEGFNARAENPTRWNEMVTTGPDYL
                     ERALESRPFMVKSSLETARLAAAPSVMVISGNNARKTEYITKSIAAGFHVLADKPMVI
                     RPSDYPQLEADFAKAKEKGVLLYDIMTERFEITTMLQRALSQQPKLFGSLQQGTPDNP
                     SITKISVHNFSKVVAGAQLKRPQWFFDPEQQGAGIVDVMTHLVDLVQWEAFPEVTLAP
                     ADARVLTARRWATPLTLEQFGRVTGAKEFPAFLGNYVKEGVLRAPANGEFTYALKGVH
                     AKVSVTWEFEAPPGAGDTHFSVMRGTQASLTIRQGKEQGYKPVLYVDRSTSVPADVHE
                     TALRAAIRKVAKTWPGIDIRREGSSFVVTVPDKYHNGHEAHFTQVTENFLKYLRSGKL
                     PDWEVPNMLTKYATIMKAYEMSK"
     gene            complement(8073..9428)
                     /locus_tag="VEW08_02045"
     CDS             complement(8073..9428)
                     /locus_tag="VEW08_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYJ39539.1"
                     /translation="MNKLSVSRRNVLLTAAVAPLIISSRAWGANSRIRVGQIGCGRIA
                     RGHDMPGVIKSELADFVAICELDAKRLADGVKIIEKSYTDRGMKPPQIRTFDNYRELI
                     ARKDIDAVVISTPDHWHAEVALAAIESGKDVYLQKPFTMTHAEGVILRDAVKRHGRIL
                     QVGSQQRSWEQFRRAAELIRSGRIGNVQRVEIGLPTDPTAPDAPPAGIPANLDFGQWL
                     GPTPFKYYTEMRVHPQADYSRPGWLRHEAHCLGMITGWGSHHYDTMHWALQFEDTGPS
                     KVSGKGEFPDPDRIWNVHGAYDVTLQYPRNVVVNVSDKHNTGLKFFGDEGWIWVTREG
                     TGATTSDPKAKGAEMPPLAASDEKLLDPNGLSVHLPVSKSHHKNWLESVHSRKQPLAP
                     AHIAHRSNSACIVSWIAMKLGRPLEWDPKTERFKNDEEANAMLTRSERAPYGALHLAK
                     A"
     gene            complement(9503..10735)
                     /locus_tag="VEW08_02050"
     CDS             complement(9503..10735)
                     /locus_tag="VEW08_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ39540.1"
                     /translation="MIAAGETLRLAGIALTRNKMRSFLTALGIIIGVGAVIFMQSIGE
                     GAKSRVRAQIEGAGTNIVMLFSGSSQGPGGRGGQGSQPTITWEDLRAIQTELSSVAAV
                     APQMNANQPVISETNTWNTQITGTTPDFFTIRNWAVSSGAIFTASDQAGAAKNAVIGQ
                     TAAENLFGPGVDPIGQIIRIRDAPFTIIGVLEKKGQNAMGSDQDDVIFIPLSAFQTRM
                     QRGLGKFVPGRIFVSAVSPEAIPRARQEIGALLRERHRLGPDDEDDFSTSSMAEMTSM
                     LTSTQDTVTTLLFSVALVSLVVGGIGVMNIMLVSVTERTREIGIRMAVGAQPADIMAQ
                     FLVEALVLAAIGGALGVIFGVGCGYLLAQKFGWTMVTRTEIVVAALAVSAGVGVLFGL
                     YPARKASRLDPIEALRYE"
     gene            complement(10732..11436)
                     /locus_tag="VEW08_02055"
     CDS             complement(10732..11436)
                     /locus_tag="VEW08_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ39541.1"
                     /translation="MALIELRNLGKIYQSGDTEFAALHDVNLSIERGEFVAITGSSGS
                     GKSTLMNLLGCLDSPSRGSYLLADRDVAKLSRIELAKVRNEFIGFVFQSFNLLKRTSA
                     AENVELPLIYAKVPIAERHRRAKEALDRVGLSHRAGHHPSQLSGGQQQRVAIARALVT
                     NPPLILADEPTGNLDSVTTIEIMKLLTDLQASGMTIVFVTHEPDVAAYAARKLLLKDG
                     GVVSDERQKPVLKEAS"
     gene            complement(11445..>11966)
                     /locus_tag="VEW08_02060"
     CDS             complement(11445..>11966)
                     /locus_tag="VEW08_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39542.1"
                     /translation="PFRGSIRDIRNAPQVVQNVVTYDAVIDVTNDDLKLKPGMTATVS
                     IVTDRRRDVLAVPNTALRFRPEGAPVVGPIAVTATGAPAGVGQGRGEGNRRRNPDGAG
                     DDDVATPMVVKRSVYVLIDGKPVPREVITGLTDGRVTEVTGGELKEGEAVIVGVAGQG
                     QRGGPQQRGPRIL"
BASE COUNT         2183 a         3771 c         3732 g         2280 t
ORIGIN      
        1 cgtcagtacc aggtcaaccc gctgcatctg tacgaggagc gccggccaaa gccgttcaag
       61 gatccgccgg agggcgccaa gtccaaccgc cggccgctca cgcactacta cctgcgaatc
      121 cgtaccaagg gcggcgtcga aggaatttac ggcatggtgg acaaggaagc cctgcccgtc
      181 ttgttaggca cgctgcgacc gctcgtcatc ggccagaacg cgctggcggt cgaacgcatc
      241 tgggaccaga tgtatcgctc gaaccgccat tcgcgcgcca gccattacat gatggccatc
      301 agctacatgg acaacgcgtt gtgggatctg cgtggccgtc atttcggcgc gccggtgttc
      361 cgcctgctgg ggggcccgac acagttgccc gtgcgcgtgt acggcagctg cctcggcttc
      421 tccatcgatc cgccgctggc cggcaagcgc gccgcacagt tgaagaagga tggattcatt
      481 caccagaaat ggtttctggg attcggccct ggcgacggcg cacgcggcat ggatctcaat
      541 gtgcagctcg tgaaagcgct gcgcgaggca gtgggcgatg acgtgcagat catgttcgac
      601 gcctaccagg gttgggacct gcaatacgcg atcgagtggt gccgaaaggt cgagcagtac
      661 cggccctact tcgtcgaaga ggccgtgccc atgtcggacc tcgagagttt catccggcta
      721 tcgaaagcca ccaccattcc ggttgccacc ggcgaacacc tgtacggccg ctgggaagcc
      781 gagcagttct tcaagacgga tgcgttgcaa ttcatccagg cggatcccga atggtgcggc
      841 ggcgtgagcg aaaccgtgaa ggttgcgcac ctcgcctcgg tgcacggcgt gaagctgctg
      901 ccgcactgtc acaacgtgca cgccgccctg cacatcgtcg cgagccagtc tccgtcggtg
      961 tgccctttcg gcgagtacct catcaatcat gtcccggaga agctccactt catgaaagat
     1021 ccgctgctca ccaccaatgg gctggtgcaa ctctcggaga agccgggctt cggaatcgag
     1081 ctcgacccgg cgaaagtgga aaagcaggaa attctggtta cgctctgacg ttcacccgcc
     1141 gagaggattg gaaatgcgaa gttttgccgc cgttgtgttg agtttgggtt cgctggttac
     1201 cgcgcacgcc gccgatatca ctgcgagtcg ccagaccgcc gcctcgaagc aggctcgcgc
     1261 cggcgaagcg gaattccgcg cgctgtacag ggagctcgtg gagatcaaca ccacgctgtc
     1321 ggtcgggtca tgcaccgagg cttccaatgc gatgaaggca cgcttgagcg ccgcgggtta
     1381 cgcggacgag cagctgcagg tcatcgtgcc tcccggacgg ccgaaagacg gcaatctgat
     1441 cgccgtgctg ccgggcagtg accagtcact gaaaccaatc ctgctgcttg cacacatcga
     1501 cgtggtcgaa gccaatcgcg ccgactggga acgcgacccg ttcaggctcg tcgaagaagg
     1561 cggatttttc tacgggcgcg gttcgtccga cgacaaggcc atggccgcgg tgttcgcgga
     1621 ctcgatggtt cgattcaagc aggaaaagta ccggccaaag cgcaccatca agctcgcact
     1681 gacctgcggt gaagaaacac cgaacacctt caatggtgtg agctacctca tcgagcacca
     1741 ccgggatctc atcgatgccg gctttgcgct caacgaaggc ggcggcgggc gatacgacca
     1801 gaagaccggc gtctatcggt atgtcgcggt gctggcgtcc gagaaggtct accaggactt
     1861 cacgctgacc acgcacaatc agggcgggca ctcgtctcgc cccacgccgg acaacgcgat
     1921 ctatcaactc gcgcatgcgc tcgacaagat cgaagcgcat caattcaagg tcgagttcaa
     1981 cgaaacctcg cgggcgtatt tcgagaagtt cggtgtcatc gagggtggcg agaaaggcgc
     2041 cgacatgatc gcggcggcga agaacggcga tgccgcagcc atcgaacgac tcaaaaagga
     2101 cccgtcctcc aacgccatcc tgcgcaccaa ctgcgtggcc acgcagctcg ccggtggcca
     2161 cgcgcccaat gctctgccgc agcgcgcgac cgccaacgtc aattgccgca tgttcccggg
     2221 ccgaagcgcg gaggaggtcc ggcaggagct catcgcggcg atcgccgatc cgggcgtcag
     2281 cgtcgagttc caggcgacgc cggaaaagcc cggcgcgggg ccggcgttgt cacgcgaagt
     2341 catgggaccg atcgaggcat tggcgcgcga catgtatccg ggcgtcgccg tgattccaac
     2401 catcagctcg ggcgccaccg acgggcgatt cctcacgccg gccggcattc cgacgtatgg
     2461 tgtctccggc atgctggggg atggcgcgac gggcaacgca cacggactca acgagcgtat
     2521 tcgcgtgcag acgctgatcg aaggacgcga gtttctctac cggctcacga aggcatacgc
     2581 gggcggaaaa tagcgcgagc ggaaatcgcg actaataacc tttcctgaag tcgaccactg
     2641 ccaccagctt gtcgccagcc gcaaagcggc gtacctgctc cttcagcagc aacatgcggg
     2701 ccgactggcc caggtccgaa agcgccgaca tgtgcggcgt gatgatgatg ttcctggctt
     2761 tccacagtgg atgatctttc ggaagcggct cggggtcggt gacatcgagg cccgcgcctg
     2821 cgatggttcc cgcgttcagc gccgcgacga gatcatcggt caccacgctg ccgccgcgac
     2881 ccacactgat gaaataggca gtcttcttca tgcgcgcgaa cattttggcg tcgaaaaggc
     2941 gcgtcgtcgc gggaaccagc ggcaatgccg ccaccaccac gtcggcctca ccgatcatgg
     3001 ccggcagctc gtcggataga cccacgcggc tgacgtaatc gggcccttcg cgcgaggtgt
     3061 tgcgcgtgcc aatcaccttc atgcccagcg cgtggccgcg cttcgcaacc tcaagtccga
     3121 tgccgccgag acccgcgacc agcagtgtct tgccggtgag tgactgtggt tgtgagcccg
     3181 tgaaattgcg gttccagccc tcgccggtcg cctgccggcc caccgacacc tgcaaggaac
     3241 gcgatagcgc gaacatcaac gcgatgctgt gttcggccat cactggaccc gcgaccgcgc
     3301 gcatgttggt cacgatcaat ccaggtctct cgagggccgg cttgcccagg cacgcttcaa
     3361 cgccggctga catgaccgca atccagcgca cgcgcttcgc tgcggcgagc acgcggtcgt
     3421 cgcaggcgat gtcgtcgccg ccgatgatgg cgtcggcgtc cgccgcttcc ttgatgaggc
     3481 cggccgcgtc agcgacgaac accatctcga tgccgggagc tacgccttcc agcgttgcac
     3541 ggatttcctg tgtgcttgtg ccagtgggat tctgcgtggc catcacgatg cgccggggtg
     3601 cgcgccagcc gcgaatgtcg cgcagcgcgg aggggctctc ggcgagccca tgctcgcgga
     3661 tcaattcaac ggctttggga tcggcggctg gtgctgcgtc cttggccaga gcgcagacag
     3721 cgaggaaacc tgatgcggct aacaaagcgg cgcgtacgtg aaacttgttc attcccatct
     3781 tcctcatcaa cgcagccgaa tcggcgccgc atgatgccac gcgtaatgga agtgaaacca
     3841 ttcaactttg tgatgtcaga tcaggccgat gtgaggtccg gcccctcctg ccaggaaacg
     3901 agttctgacc ccatctattg caggaggccg gccttcactt cttcctgtga gaggagctgc
     3961 acgggaacgg ccttcgcctt ccagatctcg ttgcagtatt cgcggatcgt tcgatcggac
     4021 gagaacttgc ccgaccgcgc ggagttcagg atggacatgc gggtccagcc ttccttgtcg
     4081 cgataggtct ggtcgaccag cgtctggcaa tccgcgtacg actggtagtc ggcgaacacg
     4141 aagtacggat cgtgatggac caggttgtcg agcagcggct ggaactgcga gctgttgccg
     4201 cgcgagaaga acccatcgcg gatcagttga aggatggcct gcagctcctc gctgttctcg
     4261 acgaagtcca tgggccggta gcccgcggcg cgacgagccg ccacctcgtg cgcttcgagg
     4321 ccgaacagga agaagttctc cacgcccact tcttcgcaga tttcgatgtt ggcgccatcc
     4381 accgtgccga tggtcagagc gccgttcatc tggaacttca tgttgcccgt gccggacgcc
     4441 tccttgccgg cggtggaaac ctgttccgac agatcggcgg ccgggtagat cttctgccca
     4501 ttggtcacgt tgaaattcgg caggtagacg accttgatga gatcgcgcac tcccggatcg
     4561 cggttgatga tctcggccgc cgcggtgatc aggcgaatga tgagcttcgc caggaaataa
     4621 cccggcgccg ccttgccgcc gaacacgaac acgcgcggct ccatcgtgaa cttcgggtcc
     4681 gttttcagcc ggtgatacag gccgatgacg tgcaggatgt tcaggtgctg gcgtttgtat
     4741 tcgtggatgc gtttgacctg cacgtcgaag atcgccgacg gatcgacgat gactcccgtg
     4801 cgctgctcga tgagcccggc gagcgccgtc ttgttggcgt gcttgatctg gcgccactcg
     4861 gcgcggaaat ccggatcgtc gaccagcggt tcgagcttgc gcagctcgta gagatcgcgg
     4921 atccagccat cgccgatgcg ctgcgtgatg caatgcgcga gacgcgggtt cgaaagcacc
     4981 atccagcgcc gcggcgtgac gccattggtc ttgttggaaa atttttccgg ccacagcagt
     5041 gcaaaatcct tcaacacgtc gcgcttgagc aattcagaat gcagttccgc gacgccgttg
     5101 atcgcgtggc tgccgacgct cgcgagattc gccatgcgca cgtaccgctc tccggcttcg
     5161 tcgatgagcg acatccgcga gatgatctgt tcatcgccga agaacgccat gcgcacttca
     5221 tcgagatggc gcgcgttgat ctcgtagatg atctcgagga ttcgcggcaa cagccgcccg
     5281 aacatcggca acggccagcg ctccagtgcc tcgggcagca gcgtgtggtt cgtgtacgag
     5341 caggtcttgc gcgtgatgtc ccaggcacgg tcccaatcca tggccatctc gtccaccagc
     5401 aaccgcatga gctccgccac cgcgatcgac gggtgcgtgt cgttcatctg gattgcgaac
     5461 ttctcgtgga accgttccgg cggaatcttc tgcacgcggc agatgcgcag catgtcctgc
     5521 agtgagcagg acacgaaaaa gtattgctgt tccagtctta attccttccc ccgggcttgc
     5581 tcgtcgttcg gatagagaac cttggtgagg ttctcgctgg ccaccttctg gttgaccgca
     5641 ccgtagtagt caccgcgatt gaacgtgccg aagtcgaagg attccggcgc ctcggccttc
     5701 cacaaccgca acgtgttcgc ggtgttcacg tggtaaccga ggatgggcgt gtcgtaggga
     5761 atgccattga cgatgtgtcc tggcacccag cgcgtacgct tgcgaccgta ttcgtcgtac
     5821 cagacctcgg tgtggccgcc gaacttcacg tcgcagcacc actcgggccg cgcgatctcc
     5881 cagggattgc cataacgcag ccacttgtcg gtcttctcca cctgccagcc gtcgacgatc
     5941 tcctgctgga agatgccgaa ctcgtagcgg atgccgtaac ccagcgccgg cacctcgagc
     6001 gtggccagtg agtcgatgaa gcaggccgcg aggcgaccca ggccgccgtt gcccaaaccc
     6061 ggctcgtctt cgtgggctaa caactcttcg aggtcgagcc cgagctcggc caccgccgtg
     6121 cgcgcggttt cgtaaatccc gaggttgatc aggttgttgc cgaggtgcgg ccccatgagg
     6181 aattccgcgg acagatacgc caccgtgcgt gagccctgct tcgtatacgc cgccgcggtg
     6241 ctgatccagc gttgcagcat gcggtcgcgc accgcatagg ccagcgccat gtagtagtcg
     6301 gtcttggtgg cgagcgccgg aaacttgccc tggatgtaga acaggttgtc gaggaaggcg
     6361 tgcttgagcg cctccttgct catggcaacg cggtcttcgt cgatttgcgg cgggctgccg
     6421 gcgacgttcg ggaggctgct tactggggga ctcatgtcgc ctcgcggatt gttgttggcc
     6481 ggactctgca agattcatgc aggaaatacg gcacgtaaat accgcaatcg cattgcaatc
     6541 tgcaccgcga cgaagcgtgc gccgaaaatc cggctccgcg gcggtgcaag taaaagggga
     6601 cagatctatt ttccggctgc cggaaaatag atctgtcccc ttttacttgc tcatctcgta
     6661 cgccttcatg atggtggcgt acttggtcag catgttcggc acttcccaat ccggcagttt
     6721 cccggaccgc aggtacttga ggaaattctc ggtcacctgg gtgaaatgcg cctcgtgccc
     6781 gttgtggtat ttgtccggca ccgtgacgac gaacgaactt ccctcgcgcc tgatatcgat
     6841 gcccggccag gtcttcgcca ccttcctgat cgcagcccgc aatgccgtct catgaacgtc
     6901 ggcgggcacg gacgtgctgc gatcgacgta caacaccggc ttgtaaccct gctccttccc
     6961 ttgtctgatc gtgagcgacg cctgggtgcc gcgcatcacg gagaagtgcg tgtctccggc
     7021 gccgggcggg gcttcgaact cccacgtcac tgacaccttc gcgtgcacac ctttcaatgc
     7081 gtaggtgaat tcgccgttgg ccggcgcgcg caatacaccc tctttcacgt agttgccgag
     7141 gaaggctgga aactccttcg ccccggtcac acgaccgaac tgctcgagcg taagcggcgt
     7201 cgcccagcgt cgcgccgtca gtaccctggc atcggcaggc gcgagcgtga cttcgggaaa
     7261 cgcctcccac tgcacgagat ccaccaggtg cgtcatcaca tcgacgatgc cggcgccctg
     7321 ctgttcgggg tcgaagaacc actgcggacg cttgagctgc gcgccggcta ctaccttgga
     7381 gaaattgtgg acgctgatct tggtgatgga ggggttgtcg ggcgtgccct gctgcagcga
     7441 gccaaacaac ttcggctgct gtgacaacgc ccgctgcaac atcgtcgtga tctcgaaacg
     7501 ctcggtcatg atgtcgtaca acagcacgcc cttctctttc gccttggcga aatccgcttc
     7561 gagttgcggg tagtcgctgg gcctgatgac catgggtttg tccgcgagca cgtggaaacc
     7621 ggccgcgatc gacttcgtga tgtactcggt cttgcgtgcg ttgttgccgg agatgaccat
     7681 cacgctgggc gctgcggcca gtcgagcggt ttccaggctg ctcttgacca tgaaaggccg
     7741 tgactcgagc gcgcgctcaa ggtaatcagg ccccgttgtc accatctcgt tccaccgcgt
     7801 gggattttcg gcacgcgcgt tgaatccttc gatgcgcttc agatgctgcg ccacatcgtc
     7861 acccgccggc gcgaacacgc gaacgtccgg cgacacgcgc ggcagcatga acttctgcac
     7921 cagcgcggca tgaaagtgac cgggatccag cgtaatgaga tacacgtcgc ttcctttctt
     7981 caccacatct gcggacgcga tgcccgcgac acatgcgaac gcggccgcca tgagggcggc
     8041 cgctccattg cttcttctct tcatgggccg aattatgcct tcgcgagatg cagcgcgccg
     8101 taaggcgcac gttcgctgcg cgtgagcatc gcgttggctt cctcgtcgtt cttgaagcgt
     8161 tcggtcttcg gatcccattc gaggggccgg cccaacttca tggcgatcca gctgacgatg
     8221 caggcgctgt tgctgcgatg tgcgatgtgc gccggcgcca gcggctgctt gcgcgagtgc
     8281 acgctctcga gccagttctt gtggtgcgac ttgctcaccg gcaaatgcac ggatagcccg
     8341 ttcgggtcga gcagcttctc gtcgctggcg gcaagcggcg gcatctcggc gcccttggct
     8401 ttcggatcgc tggtggtggc gcccgtgccc tcgcgagtga cccagatcca gccttcatca
     8461 ccaaagaatt tgaggccggt gttgtgtttg tccgaaacgt tcacgacgac gttgcgcgga
     8521 tactgcagcg tcacgtcgta ggcgccatgc acgttccaga tgcgatctgg atcagggaac
     8581 tcacctttac cgctcacctt gctcggaccc gtgtcttcga actgcagtgc ccagtgcatg
     8641 gtgtcgtagt gatgcgagcc ccagcccgtg atcatgccga ggcaatgcgc ctcgtgacgc
     8701 agccagcccg gccggctgta gtccgcctgc ggatgcacgc gcatctcggt gtagtacttg
     8761 aatggcgtgg ggcctaacca ctgtccgaaa tcgagattcg cgggaattcc cgcgggcggc
     8821 gcatcgggcg cggtggggtc cgtgggcagc ccgatctcca cgcgctgcac gttgccgata
     8881 cggccggagc ggatgagctc ggcggcccgg cggaattgtt cccaggaacg ctgctggctg
     8941 cccacctgca ggatgcgccc gtgacgcttc accgcgtcac gcaggatcac tccctcggcg
     9001 tgcgtcatgg tgaacggctt ctgtagatag acgtccttgc cggattcgat ggcggcgagc
     9061 gcgacttcgg catgccagtg atccggcgtg ctgatgacca ccgcgtcgat gtccttccgg
     9121 gcgatcagct cacggtagtt gtcgaacgtg cggatctgcg gcggcttcat gccccggtcc
     9181 gtgtatgact tctcgatgat cttgacgccg tcggcgaggc gcttggcgtc cagctcgcaa
     9241 atcgcgacga agtccgcaag ctccgacttg atgacgccgg gcatgtcgtg cccccgcgcg
     9301 atgcgtccac aaccgatctg gccgacccgg atgcggctgt tggcgcccca tgcacgggaa
     9361 gaaatgatga gtggcgcgac cgctgccgtg agcagcacat tgcgtcgcga tacggacagc
     9421 ttgttcatgg cggtcgaatc cgtacaggct ggaagggacg ccgagtataa gcggcctcca
     9481 aaccaaacca tattcgacta aatcattcgt accggagagc ctcgatgggg tccaggcgcg
     9541 acgccttgcg cgccgggtac agcccgaaca aaacgcccac gcccgcgctg actgccagcg
     9601 ccgccaccac gatctcggtg cgcgtgacca tcgtccagcc gaacttctgc gccagcaggt
     9661 acccgcagcc gactccgaaa atgacgccca gcgcaccccc tatggcggcc agcaccaggg
     9721 cttcgaccag gaactgcgcc atgatgtccg cgggctgcgc gccgacggcc atgcggatgc
     9781 cgatctcgcg cgtacgttcg gtgaccgaca ccagcatgat gttcatgacc ccgatgccac
     9841 cgacgaccag ggagacgagc gcgaccgaga acaacagggt cgtgacggtg tcctgggtcg
     9901 aggtgagcat gctggtcatc tccgccatgc tgctggtgga gaaatcgtct tcgtcatcgg
     9961 gcccgagacg atgccgctcg cgaagcaagg cgccgatttc ctggcgcgca cgcggaatgg
    10021 cttccggcga cacggcgctc acgaagatgc gcccgggcac gaacttgccg agtccgcgct
    10081 gcatgcgcgt ctggaaggcg gacagcggaa tgaagatgac gtcgtcctgg tccgaaccca
    10141 tcgcgttctg acccttcttc tcgagcaccc cgatgatagt gaatggagcg tctcgtatcc
    10201 ggatgatctg gccgatcgga tcgacaccgg ggccaaacag gttttccgcg gccgtctggc
    10261 cgatcacggc attcttcgcc gcgcccgcct ggtcggatgc cgtgaagatg gccccgctcg
    10321 acacggccca gttccggatc gtgaagaagt cgggggtcgt gcccgtgatc tgcgtattcc
    10381 aggtgttggt ttccgagatc accggctggt tggcattcat ttgcggcgca acggcggcaa
    10441 ccgaggacag ctcggtctgg atggcgcgca ggtcttccca ggtgatcgtg ggctgtgatc
    10501 cctgaccgcc gcgaccgccc ggaccctggc tcgatcccga aaacaacatg acgatattgg
    10561 tgccggcgcc ttcgatctgt gcgcgcactc gcgacttcgc gccctcgccg atcgattgca
    10621 tgaagatgac ggcaccaaca ccgatgatga tgccgagcgc ggtgagaaag gagcgcatct
    10681 tgtttcgcgt caacgcaatt cccgcgagac gcaacgtttc cccggcggca atcatgaagc
    10741 ctccttgagc acgggtttct ggcgttcgtc gctgacgacg ccgccgtcct tgagcaatag
    10801 tttgcgcgcg gcataggccg cgacgtcggg ctcatgcgtg acgaacacga tcgtcatgcc
    10861 ggaggcctgc aggtccgtga gcagtttcat gatttcgatc gtcgtgaccg agtcgagatt
    10921 gcccgtaggt tcgtcggcaa gtatcaacgg tggattggtg accaatgcgc gcgcgatggc
    10981 cacacgctgc tgctggccgc ctgataactg ggagggatgg tgaccggcgc gatgcgaaag
    11041 gccgacgcgg tcgagtgctt ccttggcgcg gcggtggcgt tcggcaatag gcactttggc
    11101 gtaaatcaat ggcagctcga cgttctcggc cgccgaggtg cgcttgagca ggttgaaact
    11161 ctggaacacg aagccgatga actcgttgcg caccttggcg agctcgatgc gcgacagctt
    11221 ggccacgtcg cgatcggcca gcagatagct gccacgcgat ggcgaatcga gacagccgag
    11281 cagattcatc agcgtcgact ttcccgaacc cgaagaaccg gtgatcgcca cgaactcgcc
    11341 acgctcgatg gacaggttca cgtcatgcaa cgccgcgaat tccgtgtcgc cggactggta
    11401 gatcttgccg agattgcgta gttcgatgag agccatgggc gcggctagag aattcgcggg
    11461 ccgcgctgct gcggcccacc gcgctggccc tggccagcga cgccaacaat gaccgcctcg
    11521 ccttccttga gctcaccacc cgtcacttcg gtcacgcggc cgtcggtgag acctgtgatc
    11581 acctcacgcg gcacaggctt tccgtctatc aggacgtaga ccgagcgctt gaccaccatt
    11641 ggcgtagcaa catcgtcatc acctgcgccg tccgggttgc gacgtcggtt tccctcgccc
    11701 cgaccctgtc caacgcccgc cggcgccccg gtcgcagtca ccgcgatcgg gcccacgacc
    11761 ggcgcgcctt caggccggaa acgcagcgcc gtgttaggca cggcgagtac atcgcggcga
    11821 cggtcggtga cgatgctcac ggtggcagtc atgccaggct tgagcttgag gtcgtcgttg
    11881 gtcacatcga tgacggcgtc ataggtgacc acgttctgca cgacctgggg cgcgttgcga
    11941 atgtcgcgga tggaaccgcg gaacgg
//
LOCUS       DASQJS010000054         3671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3482833, whole genome shotgun sequence.
ACCESSION   DASQJS010000054 DASQJS010000000
VERSION     DASQJS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3482833"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VEW08_02065"
     CDS             complement(<1..654)
                     /locus_tag="VEW08_02065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYJ39543.1"
                     /translation="MTSPASVTTLPLLNAAELAHSQRVIEHIRDFMQKHGGVIGFDAW
                     MRLALYAPGLGYYSAGATKLGSHGDFVTAPEMGSLFSRCLARQCAEVLQVTGGEILEL
                     GAGSGRMAADVLTELAAIDALPDRYCILEVSADLAERQRAHIAQLPRELAARVQWLDR
                     WSERSMRGVVLANEVLDAMPVERFILRGRPGGIDVRALGVGLGREGFEWRETSPSAEL
                     "
     gene            666..1421
                     /locus_tag="VEW08_02070"
     CDS             666..1421
                     /locus_tag="VEW08_02070"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HYJ39544.1"
                     /translation="MKDSDGSLTGKTVLITGGARRVGATIARTLHGAGANLVVHFRNS
                     SGEAEALAGELNGIRAGSSAIIQADLLDVGKLPALVEFAVRSFGALDVLVNNASTFYP
                     TKIGEITPQAWDDLMGSNLKAPLFLSQAAAPVLKKSRGLIINIVDIHSLRPLRDYTVY
                     CTAKAGLHMLTRSLAKELGPEIRVNGISPGPVMWPEHGGDEAARKKIIQRTILQKMGT
                     PEDIARTALFFAASAPFITGQILAVDGGRSVAW"
     gene            complement(1444..1974)
                     /gene="folK"
                     /locus_tag="VEW08_02075"
     CDS             complement(1444..1974)
                     /gene="folK"
                     /locus_tag="VEW08_02075"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HYJ39545.1"
                     /translation="MKALKPGHVRVFVAAGSNLEPEKNLARACAAIKHSWHDAVFSRA
                     YSNVAVGFDGPDFINLVLGFTTPQPLDAVIARLRAIETQCGRPRFAPKWASRTMDLDV
                     LLFGDRVEKTAEYTLPRPDLLKRPYMLGPMAEIAPEVMHPTANKTIGQLWKEFDRDGH
                     AMNPVELPEMVPGTDI"
     gene            complement(1971..2402)
                     /gene="folB"
                     /locus_tag="VEW08_02080"
     CDS             complement(1971..2402)
                     /gene="folB"
                     /locus_tag="VEW08_02080"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HYJ39546.1"
                     /translation="MSKVTSGPKTHTLPSSASTPDKGDRIFLHGLAVECIIGFIEWER
                     RVKQTIVLDVEMPVDCTRAAASDDVADTLDYKKVAKRLIAFVEASDFKLVETLAHRTA
                     LLILEEFGIEWVRLSINKPGAIRGSKDVGVAVLRTRSELAK"
     gene            complement(2399..3421)
                     /gene="tsaD"
                     /locus_tag="VEW08_02085"
     CDS             complement(2399..3421)
                     /gene="tsaD"
                     /locus_tag="VEW08_02085"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HYJ39547.1"
                     /translation="MRILALESSCDESAVAVYDDAAGLLSHEIHSQVDLHRVYGGVVP
                     ELASRDHVRRLLPLLKTALADAKTAPGQIDGVAYTAGPGLIGALLTGAALGRSLAYAW
                     NVPAIAVHHLEGHLLAPLLEPDPPPFPHVALLVSGGHTMLIDVAAPGSYAVLGETRDD
                     AAGEAFDKSAKLLGLPYPGGPELASLAARGRADRFDFPRPMLDRPGLEFSFSGLKTAV
                     SLTVKECAKAGAIDDATRADIAAGVQQAIVDTLIGKTLRAIEQTGYETLVVAGGVGAN
                     RSLRERLAEGAKRAGARVYYPRIEFCTDNAAMIAVAGMARLKAGKVESQAIRARATWS
                     LTELGT"
     gene            3559..>3671
                     /gene="rpsU"
                     /locus_tag="VEW08_02090"
     CDS             3559..>3671
                     /gene="rpsU"
                     /locus_tag="VEW08_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HYJ39548.1"
                     /translation="MPSVRVRENEYFDAALRRFKRACEKAGVLTELRRREF"
BASE COUNT          653 a         1205 c         1176 g          637 t
ORIGIN      
        1 gagctcggcg ctgggactcg tttcacgcca ctcgaagccc tcacgaccca ggccgacgcc
       61 cagcgcgcgc acgtcgatgc cgccgggccg gccgcgcaat atgaatcgct cgaccggcat
      121 cgcgtccagc acttcattgg ccagcaccac gccacgcatc gagcgctcgg accagcgatc
      181 taaccactgc acgcgtgcgg caagctcacg cggcagctgc gcgatgtgtg cgcgctgccg
      241 ttcggcgaga tcggcgctca cttcgagaat gcagtaacga tcgggcagcg catcgatggc
      301 agccaactcg gtgagcacat cggccgccat gcggccggaa cccgcgccga gctccaggat
      361 ttcgccaccg gtcacctgca acacctcggc gcattgccgc gcgaggcagc gtgagaacag
      421 gctgcccatc tccggcgcgg tcacgaagtc cccgtggctg ccgagcttcg ttgcgccggc
      481 gctgtagtac ccgagccccg gcgcgtacag cgccagtcgc atccaggcgt cgaagccgat
      541 gacgccgccg tgcttctgca tgaaatcgcg gatgtgctcg atcacgcgct gcgaatgcgc
      601 gagctcagcg gcgttcagca gcggcagggt tgtgacggac gcgggagaag tcatacagtg
      661 cgggcgtgaa agacagtgac ggcagtttaa ccggcaagac ggtgttgatc acgggcggtg
      721 cgcgccgcgt cggcgcgacg attgcgcgaa cgcttcacgg cgcgggcgcg aacctggtcg
      781 ttcacttccg aaactcctcg ggcgaagccg aagcgcttgc cggcgagctc aatggcatac
      841 gcgccggatc ctcggcgatc atccaggccg atctgctcga tgtcggcaag ttaccggcgc
      901 tcgtggaatt cgcggtgcgc agcttcggcg cgctcgacgt gctcgtgaac aatgcctcga
      961 cgttttaccc gacgaaaatc ggcgagatca caccgcaggc ctgggacgac ctgatgggca
     1021 gcaacctcaa ggcgccgctg ttcctctcgc aggctgctgc gccagtgctg aagaaatccc
     1081 gcgggctcat catcaatatc gtcgacattc attctttgcg accgctgcgc gactacacgg
     1141 tgtactgcac tgccaaggcc ggcctgcaca tgctcacgcg gtcgctcgcc aaagaactgg
     1201 ggcccgagat aagagtgaac ggcatttccc cggggcccgt catgtggccc gagcatggcg
     1261 gcgacgaggc cgcgcgcaag aagatcatcc agcgaaccat cctgcagaag atgggcacgc
     1321 ccgaagacat cgcgcgcacc gcgctgttct tcgcggcctc cgcgccgttc atcaccgggc
     1381 agattctggc cgtcgacggc ggccgcagcg tcgcctggtg aaaacggtgc caggcaccgt
     1441 ttgctagata tcggtgcctg gcaccatttc tgggagctcc acgggattca tcgcgtgccc
     1501 gtcgcgatcg aactctttcc ataactgacc aatggtcttg ttagccgtcg gatgcatcac
     1561 ttccggcgcg atctcggcca tcggccccag catgtatggc cgcttgagca ggtccgggcg
     1621 cggcagcgtg tattccgccg tcttctccac gcgatccccg aacagcagca cgtcgagatc
     1681 catggtgcgc gacgcccact tgggcgcgaa tcgcggccgg ccgcattgcg tctcgatggc
     1741 gcgcagccgc gcgatgaccg catccagagg ctgcggcgtg gtgaagccca gcaccaggtt
     1801 gatgaaatcc ggaccgtcga atcccaccgc cacgttgctg tacgcgcgcg agaacacggc
     1861 gtcatgccag gaatgcttga tggcggcgca ggcgcgcgcg agattttttt ccggttcgag
     1921 attgctgcct gccgcgacga agacgcgcac gtggccaggc ttgagcgcct tcacttggct
     1981 agttcggaac gcgtgcgcag cacggccaca cccacgtctt tcgaaccacg gatggcgccc
     2041 ggcttgttga tggacaaccg cacccactcg atgccgaact cctcgaggat gagcagcgcg
     2101 gttcgatgcg ccagcgtctc caccagcttg aagtccgacg cctcgacaaa cgcgatgagc
     2161 cgcttggcga ccttcttgta atcgagagtg tcggcgacgt cgtcgctggc cgcggctcgc
     2221 gtgcagtcca cgggcatttc cacgtcgagc acgatggtct gtttgactcg ccgttcccac
     2281 tcgatgaagc cgatgatgca ttccacggcc aggccgtgca ggaagatgcg atctcccttg
     2341 tcgggagtgg acgccgaact cggcagcgtg tgggtcttgg ggccgctggt gactttgctc
     2401 atgtgcccaa ttctgtcagg gaccacgtcg cccgggcgcg aattgcctgc gattccacct
     2461 tgcctgcctt gagccgcgcc atgccggcca cggcgatcat ggccgcgttg tccgtacaaa
     2521 actcgatgcg cgggtaataa acgcgcgcgc ccgcgcgctt tgcgccttcg gccaagcgtt
     2581 cgcgaagact gcgattcgca cccacgccac cggccaccac cagcgtttcg taaccggttt
     2641 gctcgatggc gcgcaacgtc ttgccgatga gcgtgtcgac gatggcctgc tgtacgccgg
     2701 cggcgatatc ggcgcgcgtg gcgtcgtcga tggcgcctgc tttcgcgcac tccttcaccg
     2761 tgagcgacac cgcggtcttg aggccggaga aactgaactc gaggccgggg cgatcgagca
     2821 tgggacgcgg aaaatcgaag cggtccgccc ggcccctcgc tgccaggctg gcgagctcgg
     2881 gcccgcccgg atacggcagc cctaacaact tggccgactt gtcgaaggct tcgccggccg
     2941 cgtcgtcgcg cgtttcgccg agcaccgcat aagagccggg cgcggctacg tcgatgagca
     3001 tggtgtggcc gccggagacc agcagcgcca cgtgcgggaa cggcggcggg tcaggctcga
     3061 gcagcggcgc gagcagatga ccctcgaggt gatgcacggc aatggccggc acgttccacg
     3121 cataggccag cgacctgccg agcgcggctc ccgtcagcag cgcaccgatg agaccggggc
     3181 cagccgtgta ggcgacgccg tcaatctggc ctggagctgt tttcgcatcg gccagagcgg
     3241 ttttgagcag cggcagcagc cggcgcacgt gatcgcgtga ggccagttcg ggcaccactc
     3301 cgccataaac ccggtgcaga tcgacctggc tgtggatctc atgcgacagc aggccggccg
     3361 catcgtcata gacggcaacc gcggattcat cgcaggagga ttcgagggcc aggatgcgca
     3421 tgtattaaga gtgagacccc atgtgaacgg ggctttgcct ttgtgctttg attttttggg
     3481 gccgttctat agaatcgcca cccctgcccc cggcaggttg tgacaaatcc ccaaacagat
     3541 caatgggttg aggtttcgat gccgagcgtt cgcgtacgag aaaacgaata tttcgatgcc
     3601 gccctgcgcc gtttcaagcg cgcgtgcgaa aaagcgggcg tgctcaccga gctgcgacgc
     3661 cgcgaattct a
//
LOCUS       DASQJS010000055         4356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1601617, whole genome shotgun sequence.
ACCESSION   DASQJS010000055 DASQJS010000000
VERSION     DASQJS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601617"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VEW08_02095"
     CDS             <1..324
                     /locus_tag="VEW08_02095"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYJ39549.1"
                     /translation="EVGTPIIFRNEWNGQVFEQKGSVLEFKPESRVKYSLFVPRPDLQ
                     DIPEHYFFMTYELTESGGVTSLLVRQEDPRPSQPDEPTGGDAGPDVLSILKELVEAKM
                     RAHEK"
     gene            380..712
                     /locus_tag="VEW08_02100"
     CDS             380..712
                     /locus_tag="VEW08_02100"
                     /inference="COORDINATES: protein motif:HMM:NF026905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB domain-containing protein"
                     /protein_id="HYJ39550.1"
                     /translation="MFTRQHADKLVSEYLDDHEVATFDFESPLPRHEDHHLQKLVIVR
                     VDEHDFGWLYFYDSSGHVETGRASDALVGNAPLIVDRVDGKLYVTGTAHPIEHYLQEY
                     RRGNRTSA"
     gene            729..1016
                     /locus_tag="VEW08_02105"
     CDS             729..1016
                     /locus_tag="VEW08_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39551.1"
                     /translation="MSRHVPWIALLAALGAGAYGCSSLTAESPLVKDKLKAVSAGYTG
                     CMPEENELSNVAPKPDGSGTWNATCKGKSFLCSAVADANNSVSFHCAPVAE"
     gene            complement(1075..1575)
                     /locus_tag="VEW08_02110"
     CDS             complement(1075..1575)
                     /locus_tag="VEW08_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39552.1"
                     /translation="MFKSLALAVVAITVLAPVWAADPPADPPEAPSAADIDKLMTQFR
                     TDLQAARADVVAKGITLSSEQAAKFWPLFQQFQKEQSAIIDAQLKSTQQYADNFAKLT
                     EAGSLEYITSLLERDEKIHGLRVKWLAKFQSAVPIGTAARVIQIDRRLGIVAQIQLAS
                     KVPLVR"
     gene            1689..2060
                     /locus_tag="VEW08_02115"
     CDS             1689..2060
                     /locus_tag="VEW08_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYJ39553.1"
                     /translation="MTREEANTIAAGWTAAWNARDVERVLAYFSEDVTFVSPTALAVV
                     GTATVRGKAALRAYWNQAMERIGALRFTLDRVVWDATARELAIVYLSDIDGRARRVSE
                     NLTLGADGLVVRAEVFHGVSG"
     gene            2057..2389
                     /locus_tag="VEW08_02120"
     CDS             2057..2389
                     /locus_tag="VEW08_02120"
                     /inference="COORDINATES: protein motif:HMM:NF022643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HYJ39554.1"
                     /translation="MKAFYLLCAIAGALIPYSQFVPWVTEHGLDVNLLMNELFSTRIG
                     AFFGLDVLVAAVALLAFIAWEGRRRRMKMLWLPIVATCVVGVSCGLPLFLFMREQQQP
                     QLQTSKVN"
     gene            2392..2838
                     /locus_tag="VEW08_02125"
     CDS             2392..2838
                     /locus_tag="VEW08_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYJ39555.1"
                     /translation="MATGTVKLHRVLRTKPERLYRAFLDAGALAKWLPPYGFTCTVHK
                     IDAKVGGTFRMSFTNFTTGNGHSFGGEYRELVPSEKIVYTDKFDDPNMPGEMTTTVVI
                     RAVSAGAELNVVQEGIPSVIPVEMCYLGWQESLKQLAHLVEPEIPD"
     gene            2855..3196
                     /locus_tag="VEW08_02130"
     CDS             2855..3196
                     /locus_tag="VEW08_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39556.1"
                     /translation="MTTDEKKLVPGVILLRIALIAGLYVIVYLVASFIPTPPGISSEM
                     RDFISTLFAVAMWIGAIAPIVAIIGVVLLRVSRHRFKALDWVSLAVGISTVLLPALFV
                     WAYSNCPNGIC"
     gene            complement(3208..>4356)
                     /locus_tag="VEW08_02135"
     CDS             complement(3208..>4356)
                     /locus_tag="VEW08_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HYJ39557.1"
                     /translation="LNAALNDEIPAIADVFRFFAGAARAMHGALAGEYLPGFTSMIRR
                     DPIGVVASIAPWNYPLMMAAWKMAPALAAGNTVVLKPSEQTPLTSLKLATICAELFPA
                     GVVNIICGRGNTVGAPLVAQPEVAMISLTGSIASGEKVLAAAVNGTKRTHLELGGKAP
                     VIVFDDADLESVVGGVRTFGFYNAGQDCTAACRIYAGPRIYDKLVADLSSAASSIKMG
                     TQEEEGVEMGPLITAKQRSRVSGFVQRATEKRHIEVTAGGKARAGSGYFYEPTVIAGA
                     RQTDEIVQKEIFGPVVSVTRFKDADQAIEWANDSDYGLASSVWTKDVGKAMKVAARLQ
                     YGCTWVNTHFMLVSEMPHGGMKRSGYGKDLSIYALEDYTAVRHVMVKF"
BASE COUNT          823 a         1295 c         1333 g          905 t
ORIGIN      
        1 gaggtcggaa ctccgatcat tttccgcaat gaatggaacg ggcaagtgtt tgaacagaag
       61 ggcagcgttc ttgaattcaa accggagtca cgcgtgaagt actcactgtt cgtcccccgc
      121 cccgacctgc aggacattcc agagcactac tttttcatga catacgagtt gaccgagagc
      181 ggaggagtga cttccctgct cgtgcgccag gaggacccgc gcccctccca gcctgatgag
      241 ccgactggtg gagatgcagg tccggatgta ctttctatct tgaaagaact ggttgaagca
      301 aagatgcgcg cgcatgagaa atagatcgcc aaacccgaat cgccgcagcg ttcagcttcg
      361 ccgttgagtg ttcgaaccaa tgttcactcg ccagcatgca gacaaattgg tttcggagta
      421 tctcgacgat cacgaggtcg caacgttcga ctttgagtcg ccgttacccc gtcacgagga
      481 tcatcacctt caaaaactgg tcatcgtgcg ggttgacgag cacgactttg gatggctgta
      541 cttctacgac agctcaggcc acgttgaaac cggccgcgcc agcgatgcgc ttgtcggcaa
      601 cgctccgctc attgtcgatc gggttgatgg aaagctctat gtcacaggca ccgctcatcc
      661 gatcgagcac tacctgcagg aatatcgccg tggaaacaga acgagcgcct gacaaatcac
      721 cgccttcaat gagcaggcac gtcccgtgga tcgcattgct tgccgcgctc ggtgcaggcg
      781 cgtatggctg ctcgtctctc acggccgagt cgccgctcgt caaagataaa ctgaaagcag
      841 ttagcgcagg ctacaccggg tgtatgcctg aagaaaatga gctttccaat gtcgcaccga
      901 aacccgacgg tagtggaacc tggaacgcga cctgcaaggg aaagtcattt ctgtgctcgg
      961 ctgttgccga cgccaacaac tccgtgtcgt tccattgtgc cccggtcgct gaatgaaaca
     1021 gctgcgtctc gcatgcattg ccgcgcgagc caccatcgcc gcggtgtaaa gcgctcagcg
     1081 cacgagcgga accttcgaag ccagctggat ctgcgcgacg atgccgagac gccggtcgat
     1141 ctgaatcacg cgggccgcgg tgccgattgg cacggcagac tgaaacttgg ccagccactt
     1201 gacgcgtaga ccatgaattt tttcgtcgcg ctcgagcagc gaggtgatgt actcgaggga
     1261 accagcctcg gtcagcttgg cgaaattgtc ggcgtactgc tgggtcgact tgagctgtgc
     1321 gtcgatgatg gcgctttgct ccttctggaa ctgctggaag agcggccaga atttggccgc
     1381 ctgctctgaa gagagtgtga ttcccttggc cacgacatcg gcgcgcgccg cctgcaaatc
     1441 ggtgcggaac tgcgtcatga gtttgtcgat gtcggccgct gaaggagctt cgggcggatc
     1501 ggccggcggg tccgcggccc agacaggagc aaggaccgtg atggcaacga cggcaagcgc
     1561 gagcgacttg aacatgactt ctcctgcgga cggctgatct gacttgccgg tcatcgttgc
     1621 atttgaggtg ctgaggctct atccaaatct cgcagtcttg gtgagtgcgc ggctagaatc
     1681 ttcgccacat gacccgtgaa gaagccaata cgatcgcagc cgggtggacc gctgcctgga
     1741 acgcgcgcga cgtcgagcgc gtgctcgcgt acttcagtga agacgtcact ttcgtcagcc
     1801 cgacggcgct agcggtggtg ggcaccgcca cggttcgggg aaaggccgcg ttacgggcgt
     1861 actggaacca ggccatggaa cgcatcggcg cattgcgttt cacgctcgac cgcgtggtct
     1921 gggatgcgac ggcacgcgag ctcgccatcg tctatttgtc cgacatcgat ggccgcgcca
     1981 ggcgcgtgtc ggagaacctg acgttgggcg cggacggcct cgtggtgcgc gccgaggtgt
     2041 ttcatggagt ttccggatga aggcgttcta cctgctctgc gccatcgccg gtgccctgat
     2101 tccttatagc cagttcgttc cctgggtcac cgagcatggc ctcgacgtga accttctgat
     2161 gaatgaactg ttctcaaccc gcatcggtgc gtttttcggc ctggacgtat tggtggcggc
     2221 ggtcgcattg ctggcgttca tcgcgtggga gggccggcgc cgccgcatga agatgttgtg
     2281 gttgccgatt gtcgccacct gcgtggtcgg cgtgtcctgc ggattgccac tgtttctatt
     2341 catgcgcgaa cagcagcaac cacagttaca aacttcgaag gtgaactagt cgtggccacg
     2401 ggcaccgtca aactacatcg cgtcctgcga accaagccgg agcggctcta tcgggctttc
     2461 ctcgacgccg gcgcccttgc caagtggctg ccgccctatg ggttcacgtg cacggtccac
     2521 aagatcgatg cgaaggtggg cggcacattc cgcatgtcgt tcacgaactt cacgaccggc
     2581 aatgggcact cgttcggcgg cgaataccga gaactcgtgc catccgaaaa gatcgtctac
     2641 acggacaagt tcgacgaccc gaatatgccg ggtgaaatga ctaccactgt cgtcatcagg
     2701 gccgtcagcg ccggcgcgga gctcaacgtg gtgcaggaag gtattccatc cgtcattccg
     2761 gtcgagatgt gctatctagg ctggcaggaa tcattgaagc agctggcaca tctcgtggag
     2821 cccgaaatcc cggattgatt gcgggagtag tcaattgacg accgacgaaa agaaactggt
     2881 tcctggcgtc atcctgctga gaatcgcgtt gatcgccggc ctctacgtca tcgtgtactt
     2941 ggtggcatcg ttcatcccga cgccgccagg catctcgtcg gagatgcgcg acttcatcag
     3001 tacgctgttt gccgtggcga tgtggatcgg cgccattgcg ccgatcgtcg ccatcattgg
     3061 agtggtgctg ctgcgtgtta gccgccaccg attcaaggcg ctcgactggg tctcgctggc
     3121 cgtcgggata tcaaccgtgt tgctccccgc tctcttcgtc tgggcgtatt caaactgtcc
     3181 caacgggatt tgctgagcac ccgcgtttca gaacttgacc atcacgtgcc tgacggccgt
     3241 gtagtcctcg agcgcataga tgctcaggtc tttgccatag cccgatcgtt tcatgccgcc
     3301 gtggggcatc tcgctcacca gcatgaagtg tgtgttcacc caggtgcagc cgtattgcag
     3361 gcgcgctgcc accttcatgg ccttgcccac gtctttggtc cacacggatg acgccaggcc
     3421 gtagtccgaa tcgttcgccc attcgatggc ctggtccgcg tccttgaagc gcgtgacgct
     3481 gactaccggc ccgaatattt ccttctgcac gatttcatcg gtctgtctgg cgccggcgat
     3541 gacggtgggc tcgtaaaaat acccgctgcc ggcgcgcgct ttaccgccgg ccgtgacttc
     3601 gatgtgtcgc ttctcggtgg cgcgctgcac gaaaccggac acacgcgagc gctgtttcgc
     3661 ggtgatgagc ggccccatct ccacgccctc ttcttcctgc gtgcccatct tgatgctgct
     3721 ggcggcgctc gacagatccg cgacgagttt gtcgtagatc ctggggccag cgtagatgcg
     3781 gcaagccgcg gtgcaatcct ggccggcgtt gtagaagcca aacgtacgca cgccgccaac
     3841 cacagactcg aggtcggcgt cgtcgaacac gatgacgggc gctttgccgc ccaactccag
     3901 gtgcgtgcgc ttggtgccat tcaccgcggc agcgagcact ttctcgccac tggcgattga
     3961 accggtcaac gagatcatcg ccacttccgg ttgcgccacg agtggtgcgc ccacggtgtt
     4021 gccgcggccg cagatgatgt tgacgactcc cgcggggaac agctcggcgc aaatggtggc
     4081 gagcttgagc gaggtgagcg gagtctgttc agagggtttg agcaccacgg tgtttcccgc
     4141 ggccagcgcc ggagccattt tccaggccgc catcatgagt ggatagttcc acggggcgat
     4201 cgaggcaacg acgccgatgg gatcgcggcg aatcatgctg gtgaagccgg gcaagtactc
     4261 gccggccagg gcgccgtgca tcgcgcgcgc ggcaccggcg aagaagcgga atacatcggc
     4321 gatcgccgga atctcatcgt tcaacgcggc gttcag
//
LOCUS       DASQJS010000056        12156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3170853, whole genome shotgun sequence.
ACCESSION   DASQJS010000056 DASQJS010000000
VERSION     DASQJS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12156
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3170853"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VEW08_02140"
     CDS             complement(<1..1218)
                     /locus_tag="VEW08_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HYJ39558.1"
                     /translation="MDVTPVQHRYVASSPRGFGDLLAAELRSFGAADVRERALGVEFT
                     GSLEVAYRACLESRVGSRVFLVVAQFPAATDASFYEAARAIDWRRHIDASRTIACDFS
                     GKHPEITHTRFGALRLKDAICDQLRDTTGSRPDVATERPAVRVHAHANGPNVTISIDL
                     SGEGLHRRGYRAQAGEAPLRENLAAGILVRAGWPEKCRNATEFLDPMCGSGTLVIEAA
                     MIAANVAPGARRHYFGFLGWVGHDRAVWEKVKHDALARELKSSLRLRGVDADASVLAA
                     ARDNAARAGVGELIQFSIGRLADAKPAGEGPGENKGFLATNPPYGVRLEDRETARALM
                     KQLGDVLRERFSGWDAVVLAGSPDAGLELGIRAERVHTVWNGALECRLLRLHVSAETE
                     KQMLHTGRGARIDE"
     gene            complement(1209..2660)
                     /locus_tag="VEW08_02145"
     CDS             complement(1209..2660)
                     /locus_tag="VEW08_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HYJ39559.1"
                     /translation="MDRAQLKVTSTSPVAFVDIETTGCTPGMHRIIDVAVIGATGDAV
                     DFEWQSLVNPGVHVPAGITALTGIDNEMLADAPPFEHVALELQQRLEGRVFVAHNVRF
                     DYGFIRREFALMGTAWRSPNLCTVRLSRALYPEMPRHNLDAVMERHGIHIENRHRAMP
                     DAQALLAFWRRLRAAWSASELQGALDVASQHASLPAALPPDLADDLPESPGVYRFFGV
                     GEDGREALLYVSRANILRERVLDHFRGGPGSPRAQKLVAGVRRVEWTETAGELGASLL
                     EAREVREGRPVFNRQPRDGGERLTWLFDDNAAAPRLVQMDANVLRSGNAFGTYRAARD
                     ARRALEGLAREHHWCLKLLGLESGPGSCFGLQVGRCKGACAGKEPAPLHMARVKIGLM
                     PLKLKAWPHEGPMLLREGAGERAQFHVIDAWQHLATVDVDDADAIRQLGSSFRRRIER
                     FDVDAYRTLTRMLRDQKLLPLPAPLSRIEDSWT"
     gene            complement(2642..4129)
                     /locus_tag="VEW08_02150"
     CDS             complement(2642..4129)
                     /locus_tag="VEW08_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HYJ39560.1"
                     /translation="MSAIQWRERAAALKPRSQVFIDGRFVSSANGATFDNVNPATGRS
                     LGVVAAGDQIDIERAVASGRAAFRRGSWSQQPPKARKKVLLKLAELMLTHREELALLE
                     TLDMGKPISDSLAVDIPSAANCIRWYAEAVDKIYDEVAPTGPNALATITREPMGVVGA
                     IVPWNFPLLMASWKIGPVLASGNSLVLKPSEKSPLTALRIAELAAEAGLPEGVFNVVP
                     GFGHTAGKALASSMGVDCITFTGSTATGKTVMQYAAQSNLKKVSLECGGKSPNIVLAD
                     CPDLDKAATAAAYAIFFNQGEMCSAGSRLLVQDSIKDAMLEKIAVVGRTMTPGDPLDP
                     ATRLGAIVDETQMKRVLSYIDAGRTDGAKVTLGGRRVREDSGGYFVEPTVFDGVLPAM
                     RIAREEIFGPVLATISFKNVDEAIQIANDVEYGLAAAVWTRDINVAHRTARALRAGTV
                     YVNCYDADDITVPFGGFKQSGIGRDKSLHAFDKYTELKTTWIELS"
     gene            complement(4151..4693)
                     /locus_tag="VEW08_02155"
     CDS             complement(4151..4693)
                     /locus_tag="VEW08_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ39561.1"
                     /translation="MDVGARLKHVRELHGLSQRELARRAGVTNGLISLIEQNRVSPSV
                     GSLKKVLDGVPMSMAEFFTLDVTSAAQVFYSASELVEIGNEAASLRLVAAQRPGRQLT
                     VLHERYEPDAATGEDMLSHRGEEGGVVVRGKVELTVGGQTRVLEAGDAYYFSSSTPHR
                     FRNVGTEVCEIISASTPPTF"
     gene            4739..6064
                     /locus_tag="VEW08_02160"
     CDS             4739..6064
                     /locus_tag="VEW08_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HYJ39562.1"
                     /translation="MAAQPEHTALSLDAYWMPFTANRQFKQKPRMLARASGMYYETPE
                     GRKILDGVAGLWCVNAGHGRQEITRAVAEQLEVMDYAPAFQVGHPAAFELANALVRIA
                     PQGIDHVFFTNSGSESVDTALKIALAFHRAKGDAGRVRLLGRERGYHGVGFGGISVGG
                     MTANRKAWPTAMIPGVDHLAHTHDLKRNAFTRGLPVHGAELADDLERLVTLHDASTIA
                     AVIVEPIAGSTGVVLPPQGYLKRLREICDKHGILLIFDEVITGFGRTGSAFAAQEFGV
                     TPDMITCAKGLTNGCVPMGAVLASKKIYDAFMQGPPGAIELFHGYTYSAHPIACAAGL
                     ATLGIYEGEKLLTRAKQIGPVFEQAVHALRGLPHVIDIRNYGLVAGIELDPIAGSPGA
                     RAFGIYLKCFERGLLLRTTGDIIALSPSLIIEPKHIEELFGILSQALKE"
     gene            complement(6054..7427)
                     /locus_tag="VEW08_02165"
     CDS             complement(6054..7427)
                     /locus_tag="VEW08_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HYJ39563.1"
                     /translation="MKNLSVGSIPGHVATMSIQMFIGMVVQVAYFVTDLKFVSMLGNE
                     QAAGFSAAGNLWFVVLALTQVLNVGTAAMVSHAVGAKQHERANLVFNQSLMLSVFMGI
                     FVLALLYAVAAPYMHMEAADAVVAQAGIDYLMGVTPGLGIQFLMISMFATLRGTGIVK
                     PTMIIQFITVGLNILFAAVLTIGWLTGKPYGAFGAGLATTFATIIGVIISAYYFYRHE
                     KYVSFHREQIKPNAEVLKRLFFIGLPIGLEFFFMALVMAVMYWVIRGFGADAQAGLAE
                     GQGAMRFIMLPAMAVAFAAAPIAGQNFGARKPERVRETFNWTVGMSIGIMLALTVFCQ
                     FGSHLLMHIFTYEEAVVSVGATMLIITSWNFAANGIIFSCSSMFQGLGNTWPTIGSSI
                     IRVFVFIAPAIWLSQQPHFDLVHVWYLSVCSMFLQAVVSFALLRREFRRKLTNLAPVG
                     AAPSFIP"
     gene            complement(7579..8277)
                     /locus_tag="VEW08_02170"
     CDS             complement(7579..8277)
                     /locus_tag="VEW08_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HYJ39564.1"
                     /translation="MKSIKTLALVTGLLAISGVSQAQFSSTWTAVSDYDFRGVTQSGK
                     DPALQASADYAFSNGLSIGAWASNIDFGNDEDMELDLYLNYVGKINDTFSWTTGGTLY
                     MYPTGDAQDDYPEIYVGFNAGAFSFKQWYSWDFGALADATDAEYTEVNYTAPINDAFS
                     LAFHAGYAWGDYWDGIEQFDYAVQANYVAGHFTIFGKFTGTDASGDGKVTDDVFNNEP
                     RFLVGVMTTFPWGE"
     gene            complement(8446..8817)
                     /locus_tag="VEW08_02175"
     CDS             complement(8446..8817)
                     /locus_tag="VEW08_02175"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ39565.1"
                     /translation="MNTPSANTQIIDFSTALDGEATTPAADRLLAGDPRQTVTNYFAD
                     ATQQFFAGRWSSSPGKWRIRYTESEFCCLTQGRVVLENGRGDRWEFGPGAGFVVPAGF
                     EGTWEVLETCTKFYAIFEART"
     gene            complement(8904..9584)
                     /locus_tag="VEW08_02180"
     CDS             complement(8904..9584)
                     /locus_tag="VEW08_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYJ39566.1"
                     /translation="MGTWTTKFRKPAFVAGLLAISLAANTAFAKPPGDSGSKRGGAGF
                     ASGAIIGGAAAGPIGMVVGGAVGAVMGERSHKKSEAIAARKAEAALLASELESRSNTL
                     SEIESKAGAVGSTVQFRTGQTAVRDSDRARIAKLGALVADLKDVRVRVSGFADSRGNE
                     ELNLSLSQERAEMVALELEKAGVSKERMIIEAMGERFASSEAVADDQAFERCVEIRFE
                     SGTSLASN"
     gene            complement(9769..11139)
                     /locus_tag="VEW08_02185"
     CDS             complement(9769..11139)
                     /locus_tag="VEW08_02185"
                     /inference="COORDINATES: protein motif:HMM:NF019554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ39567.1"
                     /translation="MAETHPLTSVLSCPRCQRGPVTLARAGWMCGACSSGYPVIGEVP
                     WLFPEPRQALAEWRGRLGFLTQHLASEAAAMRASLGDVALEAATRRRLEHVARANEDQ
                     IERLRQLLTPLGLDQAGIAETTHQGLGTRLPTEQGLTNYYVNLHRDWSWGVEENQAAM
                     SELRALLGGNLEGLGRVLVQGAGAGRLAYDLHAASDATLTVATDFNPLLLFAARELFA
                     GRTVELYEFPIAPRRIEDHAVLRRLAAPAPARPGLELVAADALQPPFADGAFDTVVTP
                     WFVDIIGEPFARVAARVNLALKPGGRWINFGSLAFSRAAYAERLSLEEVSAYIPKAGF
                     AGFEVREASIPYMRSPASRHARIESTISWVANKVGPPAETPRARVLPDWLLQPDKPVP
                     RTPAFEMQAISSRVHAFLLALINGERSMRDMARVLVEQRLMPAHEAEAQVRIFLARLH
                     EQSESQ"
     gene            11267..>12156
                     /locus_tag="VEW08_02190"
     CDS             11267..>12156
                     /locus_tag="VEW08_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYJ39568.1"
                     /translation="MDHVYPPGPATVPPNLTAATAAYKHRAWLAMGGLAAFIVLYFAL
                     SGWFAWTSWRLFSGMFGQGGQFDLWQFIAAVCAAFLAVFMLKALFFIQHRFDVEDIEI
                     TRQDQPRLFEFIDRLADEARAPRAHKVYLSARVNAAVFYDLSLLNLIIPSKKNLEIGL
                     GLVNVVTLGELKAVLAHEFGHFAQRSMAVGRWVYISQQIAGHIVARRDALDKLLQQLS
                     RIDLRVAWVGWVLSIIVWSIRSMMDVLFRGVLLAQRALSRQMEFQADLVAVSLTGSDA
                     LIHALHKLSAADDAWDKALE"
BASE COUNT         2197 a         3945 c         3867 g         2147 t
ORIGIN      
        1 ttcgtcgatg cgcgcgccac ggcccgtgtg cagcatctgc ttttccgttt ccgctgaaac
       61 gtgcaggcgc agcaggcggc actcgagcgc gccgttccac accgtgtgca cgcgctcggc
      121 gcggatgccg agctcgaggc ctgcatccgg ggaacccgcg agtaccaccg cgtcccagcc
      181 ggaaaagcgc tcgcgcagaa cgtcgcccaa ctgtttcatc aaagcccgcg cggtctcgcg
      241 gtcttcgagg cgcactccgt acggaggatt ggtggcgaga aagcccttgt tctcgcctgg
      301 gccttctccc gcgggctttg catcggccag ccggccgatg ctgaactgaa tgagttcacc
      361 cacgccggcg cgcgcggcat tgtcacgcgc cgccgccagt acggatgcgt ccgcatcgac
      421 accgcgcaat ctcagggaag acttcagctc gcgtgcgagc gcatcgtgtt tcactttttc
      481 ccacacggcg cggtcgtgac ctacccagcc caggaaaccg aaatagtggc ggcgcgcgcc
      541 gggagcgacg ttggccgcga tcatggccgc ttcgatgacc agcgtgcccg agccgcacat
      601 gggatcgaga aactctgtcg cgttcctgca cttctccggc cagccggcgc gcaccaggat
      661 tcccgcggcc aggttctcac gcagtggcgc ctcgccggcc tgagcgcgat agccgcgccg
      721 atgcaggccc tcgcctgaca ggtcgatgga gatggtgacg ttcggaccgt tggcatgagc
      781 gtgcacacgc accgcgggcc gctcggttgc gacgtcgggg cggctgccgg tggtgtcgcg
      841 aagctgatcg cagatggcgt ccttgaggcg caacgcaccg aagcgcgtgt gcgtgatctc
      901 cggatgtttg ccggagaaat cgcaggcgat ggtgcgcgac gcatcgatat gtctgcgcca
      961 gtcgatggcg cgcgccgctt cgtagaagct tgcgtcggtg gcggccggaa actgcgccac
     1021 caccaggaac acgcggcttc cgacgcgtga ctccaggcag gcgcgatacg ccacttcgag
     1081 cgacccggtg aattccacgc cgagcgcgcg ctcgcggacg tcggcggcgc cgaagctgcg
     1141 cagttcggcg gctaacaagt cgccaaagcc ccgggggctc gacgccacgt agcgatgctg
     1201 aaccggcgtc acgtccacga gtcctcgatg cgcgacaggg gcgcaggcaa tggcagaagc
     1261 ttctggtcgc gcaacattcg ggtcagcgtg cgataggcat cgacatcaaa gcgttcaatg
     1321 cgacgcctga aactcgaacc cagctgcctg atggcgtccg cgtcatcgac gtcgaccgtg
     1381 gcgaggtgct gccaggcatc gatgacgtga aactgggcac gttcacccgc cccttcgcgc
     1441 aacaacatgg gtccttcgtg cggccaggcc ttgagcttga ggggcatgag cccgatcttg
     1501 actctcgcca tgtgcagtgg cgcgggctcc ttgcctgcgc aggcgccctt gcaacggcct
     1561 acctgcagac cgaagcacga gcccgggccg ctttccaggc ctaacaactt caggcaccag
     1621 tgatgctcgc gcgccagtcc ttcgagcgcg cggcgggcat cgcgagcggc gcgataggtg
     1681 ccgaaggcat tgcccgagcg caacacattc gcatccatct gcacgagacg cggtgcggcc
     1741 gcgttgtcgt cgaacagcca cgtgagccgt tcgccaccat cgcgcggctg gcggttgaat
     1801 accggccggc cctcgcgaac ttcgcgtgct tcgagcaacg atgcgccgag ctcacccgcg
     1861 gtttcggtcc actcgacacg ccgaaccccg gccaccagct tctgcgccct gggcgagccc
     1921 gggccaccgc gaaaatgatc gagtacccgt tcgcgcagga tgttcgccct ggagacatat
     1981 agaagagcct cgcgaccgtc ctcgcccacg ccgaaaaatc ggtacacccc cggcgattcc
     2041 ggaaggtcgt ccgcaaggtc cggcggcaac gccgccggca gcgaggcgtg ctggcttgcg
     2101 acgtcgagcg cgccctgcag ctccgatgcg gaccacgccg cgcgcagcct gcgccagaac
     2161 gccagcaacg cctgtgcgtc gggcatggcg cgatggcggt tctcgatgtg gatgccgtgc
     2221 cgctccatga cggcatcgag attgtggcgc ggcatctccg gatagagcgc gcgcgacagt
     2281 ctcaccgtgc acaggttcgg gctgcgccac gctgtgccca tgagtgcgaa ttcgcgccgg
     2341 atgaagccat agtcgaagcg cacgttatgc gccacgaaca cgcgaccttc gagacgctgc
     2401 tgcagttcga gcgcgacatg ctcgaacggc ggcgcatcgg cgagcatctc gttgtcgatg
     2461 ccggtgagcg cagtgatgcc ggcgggcacg tgcacacccg gattcaccag gctctgccat
     2521 tcgaagtcga ccgcgtcgcc cgtggcgcct atgacggcga cgtcgatgat gcgatgcatg
     2581 cccggcgtgc aacccgtcgt ttcgatgtcg acgaaggcta cgggtgaggt gctcgtgact
     2641 ttcagctgag ctcgatccac gtggtcttga gctcggtgta cttgtcgaag gcgtgcaacg
     2701 acttgtcgcg gccgatgccg gattgcttga acccgccgaa gggaacggtg atgtcgtcgg
     2761 cgtcataaca gttcacgtag accgtacccg cgcgcagcgc gcgcgccgta cgatgcgcaa
     2821 cgttgatgtc gcgcgtccac accgccgcgg caaggccgta ctccacgtcg ttggcgatct
     2881 ggatcgcctc gtcgacgttc ttgaacgaaa tggtggcgag cacggggccg aagatttctt
     2941 cgcgcgcgat gcgcatcgcc ggcagcacgc cgtcgaacac cgtgggctcc acgaagtaac
     3001 cgccgctgtc ttcacgcacg cgtcttccac ccaaggttac tttcgcgccg tcggtcctgc
     3061 ccgcgtcgat gtacgacagc actcgcttca tctgcgtctc gtcgacgatc gccccgagcc
     3121 tggtcgccgg atcgagcgga tctccaggcg tcatggtgcg gcccacgaca gcgatcttct
     3181 ccagcatcgc atccttgatg gaatcctgca ccagcaggcg cgagccggcc gagcacattt
     3241 cgccctggtt gaagaagatg gcgtaggccg ccgcggtagc cgccttgtcg agatccgggc
     3301 aatcggcgag cacgatgtta ggcgacttgc cgccacactc gagcgagacc ttcttgagat
     3361 tggattgcgc cgcgtactgc atcactgtct tgccggtggc ggtggagccc gtgaaggtga
     3421 tgcaatccac gcccatggac gaggccagcg ccttgcccgc cgtgtgcccg aatccgggta
     3481 cgacgttgaa tacaccttcg ggcaggcccg cctccgctgc cagctccgcg atgcgcagcg
     3541 cggtcagcgg cgatttctcc gaaggcttga gcaccagcga attgccgctg gcgagcacgg
     3601 gcccgatctt ccacgaagcc atcagcagcg gaaagttcca cggcacgatg gcgccgacga
     3661 cccccatcgg ttcacgcgtg atggtcgcca gcgcattcgg acccgtgggc gcgacctcgt
     3721 cgtaaatctt gtcgacggct tcggcatacc agcgtatgca gttcgcggcg gaaggtatgt
     3781 cgacggcgag gctgtcggaa atgggcttgc ccatgtccag cgtctctaac aaagccagct
     3841 cttcgcggtg cgtgagcatg agctctgcca gtttcagcag gaccttcttg cgcgccttcg
     3901 gtggctgctg cgaccagctg ccacggcgga atgcggcgcg gccgctcgcg acggcccgct
     3961 cgatgtcgat ctggtcgccg gcggcaacca cacccagcga acgaccggtg gccgggttga
     4021 cattgtcgaa cgtggcgccg ttcgccgacg agacgaaacg gccgtcgatg aacacttgcg
     4081 aacgcggctt caaggcggcg gcgcgttcgc gccattggat tgcggacacg gggggattca
     4141 cgggcatgat ttagaaggtc ggcggggtgg aggccgagat gatctcacag acttccgtac
     4201 ccacattgcg aaagcgatgc ggcgtggaac tggagaagta ataggcatcg ccggcctcca
     4261 ggacacgcgt ctgcccgccc acggtgagct ccaccttgcc gcgcaccacg actccgccct
     4321 cctcgccgcg atgcgagagc atgtcctcgc cggtggcggc atcgggctca taacgctcgt
     4381 gcagaacggt gagctgccgc ccggggcgtt gggccgcgac cagccgcagc gaagcggcct
     4441 cgttgcctat ctccaccagt tcgctggcgg aataaaacac ctgcgcggcg ctagtcacat
     4501 cgagcgtgaa gaactccgcc atggacatcg gcacgccatc gagcaccttc ttcagcgagc
     4561 ccaccgaggg gctgacgcgg ttctgctcga tcagcgagat gaggccgttg gtcaccccgg
     4621 cacggcgcgc cagctcgcgc tgcgacaggc catgcagttc gcgtacgtgt ttgagccttg
     4681 ctccgacgtc tatcgacatg attgggatta tgatcttggg caactgccgg agtcgaacat
     4741 ggccgcccaa cccgaacata cagccctatc cctcgacgct tactggatgc cgtttacggc
     4801 gaatcgccag ttcaagcaga agccgcgaat gctggcgcgc gcgtcaggca tgtactacga
     4861 gacgcccgaa gggcggaaga tcctcgacgg cgtcgccggg ctctggtgcg tcaatgcggg
     4921 ccacgggcgg caggaaatta cgcgggcggt ggccgaacag ctcgaggtca tggactacgc
     4981 gccggcgttc caggtgggcc atccggcggc cttcgaactg gccaatgccc tggtgcgtat
     5041 cgcgccccag ggaatcgacc atgtgttctt cacgaattcc ggctccgagt cggtcgacac
     5101 cgcgctcaag atcgcgcttg cattccacag agcgaaaggt gacgccgggc gcgtaaggtt
     5161 gctagggcgc gagcgtggtt atcacggcgt ggggttcggc ggcatcagcg tgggcggcat
     5221 gaccgccaat cgcaaggcct ggccgacggc catgatcccg ggcgtcgatc acctggcgca
     5281 cacgcacgat ctcaagcgca acgcgttcac gcgtggcctt cccgttcacg gtgcggaact
     5341 ggcggatgat ctcgaacgac tcgtcacgct gcacgacgca tcgaccattg cagccgtcat
     5401 cgtcgaaccg attgccggtt cgaccggcgt ggtattgccg ccgcagggtt atctcaagcg
     5461 gctgcgcgag atctgcgaca agcacggcat cctgctgatc ttcgacgagg tcatcaccgg
     5521 cttcggccgc accggatcgg ctttcgccgc gcaggagttc ggcgtgacgc ccgacatgat
     5581 cacctgcgcc aagggcctca ccaacggctg cgtccccatg ggcgcggtgc tggcatcgaa
     5641 gaagatctac gacgctttca tgcagggacc gcccggcgcc atcgaactgt tccacggcta
     5701 cacctattcc gcacatccca tcgcctgcgc cgcggggctg gcgacgctgg gtatctacga
     5761 aggcgagaag ttgctgacgc gcgcgaagca gatcgggccc gtgttcgaac aggcggtgca
     5821 tgcgctgcgc ggcttgccgc acgtcatcga catccgcaac tacgggctgg tcgcgggcat
     5881 cgagctcgat cccatcgccg gctcaccggg cgcgcgcgct ttcggcatct acctcaagtg
     5941 cttcgagcgc ggcctgctgt tgcgcacgac cggggacatc atcgcgctct cgccttcgct
     6001 catcatcgag cccaagcaca tcgaagagct gttcggcatc ctgtcgcagg cgctcaagga
     6061 ataaaggagg gggctgcccc caccggcgcg aggttcgtga gcttgcggcg gaactcgcgg
     6121 cgcaacagcg caaagctcac gacggcctgc agaaacatgc tgcataccga caggtaccac
     6181 acgtgcacca ggtcgaaatg cggctgttgc gacagccaga tcgccggcgc gatgaacacg
     6241 aacacgcgaa tgatgctcga gccgatggtc ggccaggtat tgccgagccc ctggaacatc
     6301 gacgagcagc tgaagatgat gccgttcgcg gcgaagttcc atgacgtgat gatgagcatg
     6361 gtcgcgccga cggataccac cgcttcctcg tacgtgaaga tgtgcatcag caggtgcgag
     6421 ccgaactgac aaaaaacggt gagtgccagc atgattccga tgctcatgcc gacggtccag
     6481 ttgaatgtct cgcgcactcg ttcgggtttg cgcgcgccga aattctgccc ggcgatcggc
     6541 gccgcggcga aggccaccgc catggcgggc aacatgatga agcgcatcgc gccctgccct
     6601 tcagccagac ctgcctgtgc gtccgcgccg aacccgcgga tcacccagta catgacggcc
     6661 atgacgagcg ccatgaagaa aaactcgagc ccgatgggca ggccgatgaa gaagaggcgc
     6721 ttcagaacct ccgcgttggg cttgatttgc tcgcggtgga acgacacgta tttctcgtgc
     6781 cggtagaagt aatacgcgct gatgatcacc ccgatgatcg tcgcgaacgt ggtggcgagc
     6841 ccggcgccga acgcgccata cggcttgccc gtgagccagc cgatggtgag caccgccgcg
     6901 aaaagtatgt tcagcccgac agtgatgaac tggatgatca tcgtcggctt cacgatgccg
     6961 gtgccacgca aagtcgcgaa catcgagatc atgaggaact ggatgccgag gcccggcgtt
     7021 acgcccatca gatagtcgat gccggcctgc gcaaccaccg catcggccgc ttccatgtgc
     7081 atgtatgggg ccgccaccgc atacagaagt gcgagcacga agatccccat gaacaccgag
     7141 agcatgagcg actggttgaa cacgagattc gcgcgttcgt gctgcttcgc gccgaccgcg
     7201 tgcgagacca tggccgccgt gccgacgttc aggacttgcg tgagcgcgag tacgacgaac
     7261 cagaggttgc ccgcggcact gaagcccgcg gcctgttcgt tgccgagcat ggagacgaac
     7321 ttcaggtcgg tgacgaagta cgccacctgc acgaccatac cgatgaacat ctggatcgac
     7381 atcgtcgcga catgtcccgg gatcgaacca acgctcaggt ttttcatgcg ggaaccaaaa
     7441 tgggggtggc ctaaatgaca ggctcccgct gaatcgtcaa ctgccgcgcc aagcccaaaa
     7501 agaaaaggcc ggtctcgcga ccggcctctt gcttcaagtc agcgtgaacc gccgatcttg
     7561 ttcttcaatt gacagtgatc attcgcccca cgggaacgtg gtcatgacac cgacgaggaa
     7621 gcgcggctcg ttgttgaaga cgtcgtcggt gaccttccca tcgccactcg cgtcagttcc
     7681 ggtgaacttg ccgaaaatag tgaagtggcc ggcaacgtag ttggcctgca cggcatagtc
     7741 gaattgctcg atgccatccc agtagtcgcc ccaggcgtag cccgcatgga acgccagtga
     7801 gaaggcatcg ttgatcgggg cggtgtagtt cacttcggtg tactccgcgt cggtggcgtc
     7861 agccagcgca ccgaaatccc acgagtacca ctgcttgaag ctgaaggcac cggcgttgaa
     7921 gccgacgtag atttccggat agtcgtcctg cgcatcgccg gtcgggtaca tgtacaacgt
     7981 accgcccgtg gtccacgaga acgtatcgtt gatcttgccg acgtagttca ggtagagatc
     8041 cagttccatg tcttcgtcat taccgaagtc gatgttcgac gcccacgcgc caatggagag
     8101 tccattggag aacgcgtagt cggcgctggc ttgcagggct ggatcttttc cgctctgcgt
     8161 gacgccacgg aagtcgtagt cgctcacggc ggtccaggtc gagctgaatt gagcttggga
     8221 aacgccgctg atggccagaa ggccagtcac gagcgccaac gttttaatcg atttcatttg
     8281 ctcctccgag agctataggg gccatttacc taagcaaagg ctgtgccatt ctcgtcctag
     8341 tagcgtgatg attctttaga tttcaacgcg ttagacgtat gcacaaaaaa tacacgtaag
     8401 tacgtggcgc ggattatgcc gtaaacccgg agcgcgatgc acgatttagg tgcgcgcctc
     8461 aaagatggcg taaaacttgg tgcaggtttc caggacttcc caggtgccct cgaaccctgc
     8521 gggcacaacg aagccggcac cgggaccgaa ttcccacctg tcgccccgcc cattttcgag
     8581 cacgacccga ccctgcgtga ggcaacagaa ctccgactcc gtgtaacgga tgcgccactt
     8641 gcccggcgag ctcgaccagc gcccggcgaa gaactgctgc gtggcatcgg cgaagtaatt
     8701 ggtgacggtt tgccgcgggt cgccggcgag caatcgatcg gcggctggcg tggtcgcttc
     8761 accgtccagc gccgtcgaga aatcgatgat ttgcgtgttc gcggatggag tgttcatggc
     8821 tggattatgg cgcgaaccca ttccggtggc gggcggcacg accacaacgg gtcgtgccac
     8881 ccgggcacca tcatcggaac cgctcagttg ctggcgagcg aagtgcccga ttcgaagcga
     8941 atctcgacgc agcgctcaaa ggcctggtca tccgcgacgg cttcgctgct ggcgaatcgc
     9001 tcgcccatgg cctcgatgat catgcgttcc ttggacacgc cggccttctc gagctcgagc
     9061 gcaaccattt ccgcacgctc ctgcgacagg ctcaggttga gctcctcgtt gcctcgtgaa
     9121 tccgcgaaac ccgagacgcg gacacgcacg tccttgaggt cggcaaccag cgcgccgagt
     9181 ttggcgattc gtgcgcgatc gctgtcgcga acggcggtct gaccggtgcg gaattgcaca
     9241 gtcgagccga ccgcgcccgc cttgctttcg atctcgctca gcgtattgct ccgcgactcg
     9301 agttcgctcg cgagcagcgc cgcctcggcc ttgcgggccg cgatcgcttc gctcttcttg
     9361 tgactgcgct cgcccatcac cgcgccgacc gccccgccga ccaccatgcc gatcggcccg
     9421 gcggccgcgc cgccaatgat ggcgccgctt gcgaagccgg cgccgccgcg cttcgagccg
     9481 ctatcgcccg gcggcttcgc aaaggcggtg ttcgccgcga ggctgattgc caacaggccc
     9541 gcaacgaacg cgggtttacg aaatttggta gtccaggtgc ccatgtgccc tctccagtgg
     9601 ttagaaactc gagagccgtt ttgctctcga ggttcattac gcaccgggat cgcgcaatcg
     9661 aactggcagc tcgcgggcga accggcgatg gatcgtggca aattgtggat tggtaggtgc
     9721 cgggtgggga atgtggggag tggactcccc acattcccca cccggcacct actggctttc
     9781 gctttgctcg tgcaggcgcg cgaggaagat gcgcacttgc gcttcggctt catgagcagg
     9841 catcaggcgc tgctccacca gcacccgcgc catgtcgcgc atggagcgct ctccgttgat
     9901 cagcgccagc aggaatgcgt gcacgcggct ggaaatggcc tgcatttcga acgccggcgt
     9961 gcgcggcacg ggtttgtcag gctgcagcag ccagtcggga agcacgcggg cacgcggggt
    10021 ctccgcgggc gggcccacct tgttagccac ccagctgatg gtgctctcga tgcgcgcgtg
    10081 ccggctggcc ggtgagcgca tgtagggaat cgaggcttcg cgcacttcga agccggcgaa
    10141 cccggcctta ggaatatatg cgctcacctc ttcgaggctc aggcgctcgg cgtaggcggc
    10201 gcgggaaaac gccagcgagc cgaagttgat ccagcgccca cccggtttga gcgcgaggtt
    10261 gacgcgcgcc gccacgcgcg caaaaggttc accgatgatg tcgacgaacc acggcgtgac
    10321 gacggtatcg aatgcgccgt cggcgaacgg cggctgcaat gcatccgcgg caaccagttc
    10381 gagcccaggc ctggccggtg cgggcgctgc caggcgccgc aacacggcgt gatcctcgat
    10441 tcggcgcgga gcaatcggaa attcgtacag ttcgaccgtg cgcccggcga acagctcgcg
    10501 tgccgcaaac aacagcagcg gattgaaatc cgtggccacc gtgagcgtcg catcggaggc
    10561 cgcatgcaga tcgtaggcca gccgcccggc cccggcgccc tgaaccagta cgcggcccag
    10621 gccctcgagg ttgccgccaa gcagcgcgcg aagctcgctc atggccgcct ggttctcctc
    10681 cacgccccat gaccagtcgc ggtgcagatt cacgtagtag ttagtcaggc cctgttcggt
    10741 gggcagacgc gtgccaagac cctggtgcgt ggtctcggcg atgccggcct gatcgaggcc
    10801 gagtggcgtg agcagctggc gcagacgctc gatctgatct tcgttcgcgc gcgcgacatg
    10861 ctcgagccgc cggcgcgtag ccgcttcgag cgcgacatcg cccaggctgg cgcgcatcgc
    10921 cgcggcctct gatgccagat gctgcgtcag gaaacccagg cggccacgcc attccgccag
    10981 cgcctggcgc ggctcgggga acagccatgg aacctcgccg atgaccggat atccgctgct
    11041 gcacgcgccg cacatccagc cggcgcgcgc cagcgtgacc gggccgcgct ggcagcgcgg
    11101 acaggaaagc acactcgtca atggatgggt ttcagccaaa gcgcggcaat gctggcggtc
    11161 aggggggctg cggtcaaatg tccgagttct cattcgtgtg acagatcact tccagggcgc
    11221 actggcctcg cgcaaactgg gcgccaggaa atcgagggag ttctggatgg accacgtcta
    11281 cccaccgggc ccggccaccg tgccgcccaa tctcaccgcc gcgaccgccg cgtacaagca
    11341 tcgtgcctgg ctggccatgg gcggcctcgc cgcattcatc gtgctgtatt tcgcgttgtc
    11401 cggctggttc gcatggacct cgtggcgtct gttcagcgga atgttcggcc agggtggcca
    11461 attcgacctc tggcaattca tcgccgccgt ctgcgccgct ttcctcgcgg tattcatgct
    11521 caaggcgttg ttcttcattc agcatcgctt cgacgtcgaa gacatcgaaa tcacgcgcca
    11581 ggaccagccg cgattgttcg aattcatcga ccggctcgcc gacgaggccc gcgcaccgcg
    11641 agctcacaag gtctatcttt cggcgcgggt caacgccgcg gtgttctacg acctttcact
    11701 gttgaatctc atcattcctt cgaagaagaa cctcgagatc ggcctgggac tcgtcaacgt
    11761 ggtgacgctc ggcgaactca aggccgtgct ggcgcacgaa ttcggtcatt tcgcgcaacg
    11821 atccatggcc gtcggccgct gggtttacat ctcgcaacag atcgccggcc acatcgtggc
    11881 gcgccgcgat gcgctcgaca aactgctgca gcagctgtcg cgcatcgacc tgcgtgtcgc
    11941 ctgggtgggc tgggtactct ccatcatcgt ctggtcgatc cgatcgatga tggacgtgct
    12001 gttccgcggc gtgctgctgg cgcagcgcgc gctgtcgcgg cagatggaat tccaggcgga
    12061 cctggtagcc gtgtcactga ccggcagcga tgcgctgatc cacgcgctgc acaagctcag
    12121 cgcggccgac gatgcctggg acaaggcgct ggaatt
//
LOCUS       DASQJS010000057         5795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2541636, whole genome shotgun sequence.
ACCESSION   DASQJS010000057 DASQJS010000000
VERSION     DASQJS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2541636"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..982)
                     /locus_tag="VEW08_02195"
     CDS             complement(74..982)
                     /locus_tag="VEW08_02195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYJ39569.1"
                     /translation="MNIIFFSRREGRARHFNLAHPFAVAAFAAVLVAILATAFSLGFK
                     IGESKAPPRGVASWTATLAEQQAEIKELKAELQRRVDAVAMRIGQMNAHVIRLDALGK
                     RLTQMANIDNREFDFESQPAVGGPESDIGAAMQAPDLNSLLNGLERKISSRDAQLAAL
                     ENALLSKKLDEQIRPDGRPVRAGHISSYFGERQDPFNGHQAFHKGIDFASPRGSEVIA
                     VAAGVVTFSGEKTGYGTLVEVSHGNGLITRYGHNQAVAVGVGRTVSRGDILAFVGSTG
                     RSTGPHVHFEVLKNGRQIDPLTFIGR"
     gene            complement(1496..3190)
                     /locus_tag="VEW08_02200"
     CDS             complement(1496..3190)
                     /locus_tag="VEW08_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HYJ39570.1"
                     /translation="MDFDAIVIGSGISGGWAAKELCERGMRTLLIERGRHVTHKTDYQ
                     DFAAPWELAHRGQVPEDEVRRDYAIQSTCYAFNSATRQWWVKDSEHPYLTPEGREFLW
                     LRGYHLGGRSLTWARQTYRFSDIDFSANSKDGHGVDWPIRYADISSWYDHVETFAGIS
                     GNSEGLEQLPDGRFQKPMDLNCAELDFKRKLESSHPSRRVTIGRCAHLTEPTAEHIAL
                     GRGPCQLRNVCERGCGYGGYFSTLSATLPAAERTGNLTVVTDAVVETLDYDHAKRRIS
                     AVRVIDAKTKQRRSYQARVVFLCASTIPTAQILLHSKSEFFPKGLANRSDQVGRNLMD
                     HVHGIGAMGNVPGFLDRYYYGRRPTGIYIPRYANVTEDSRGEYTRGWAFQGKGERPNW
                     DRALDSPGVGAQLKADLREPGPWQMVLFGFAEMLPRADNRITLHESRTDSSGLPLVHI
                     DCTFGENERKLAARADEDAQAMLRAAGCTNVRALFPHLSPPGTSVHEMGTARMGLDPA
                     TSVLNRHNQAHDIPNLFITDGSCMASSGCVNPSLTYMALSARAAHYAADRLASGEL"
     gene            complement(3206..3772)
                     /locus_tag="VEW08_02205"
     CDS             complement(3206..3772)
                     /locus_tag="VEW08_02205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HYJ39571.1"
                     /translation="MEAGNSEKINRRALLAGAVFLLGGAAALTRFSRPTGDKADTAGP
                     VLSAEQFAQLEQVSETMIPATDTAGAIGAGVPAFIREMLAQWGSAATRTDIANVLEAI
                     EQQAWSRFGMAFLEMPPERRLEVVKEFDAARIGAHDGAYRKFKWLVLIGYYHSEVGAT
                     HELRFELVPGAWRACLPVTEVGRASAFY"
     gene            complement(3923..4849)
                     /gene="lpxC"
                     /locus_tag="VEW08_02210"
     CDS             complement(3923..4849)
                     /gene="lpxC"
                     /locus_tag="VEW08_02210"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004881498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HYJ39572.1"
                     /translation="MLGQRTLKNSIRAQGVGMHTGQKILMTLRPAAPDTGIVFRRTDL
                     DPPAEVRAHALNVGDTQLGTTLINGEARVSTVEHLLSAFAGLGIDNAHVDVSAPEVPI
                     MDGSAGPFVFLLQSAGFEEQRTPKKFVRIKQRVRVEDGDKWAEFRPFDGFKVNFEIEF
                     NHPLFKRRGQKASMDFSTTSYLKEVSRARTFGFMRDLEMLRARNLALGGNFDNAIVLD
                     DFRVLNEDGLRYEDEFVKHKILDAIGDLYLLGHSLIGEFSGYKSGHKLNNNLLRKLIS
                     EPSTWEAVTFEKPADAPIAYVQPPPVAVGSAF"
     gene            complement(5007..>5795)
                     /locus_tag="VEW08_02215"
     CDS             complement(5007..>5795)
                     /locus_tag="VEW08_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HYJ39573.1"
                     /translation="RTLVANHGLAELGKYVDSLITIPNQKLLTVLGAKTTLLDAFKSA
                     NHVLQGAVQGIAELITRPGLINVDFADVRTVMSETGMAMMGSGVARGENRAKEAAEMA
                     VSSPLLEDINLSGAHGILVNVTAGMDLSIGEFQEVGNIVKQFASEDATVVVGTVIDPE
                     LSDEMRVTVVATGLGKPEIAVSGRSPFGRDVPTLSKAVPQAAAPAAEPSMRVVPRRPL
                     TAADYAALDKPAVQRQRAVGDGLHASAESEDVLDIPAFLRRQAD"
BASE COUNT         1038 a         1833 c         1868 g         1056 t
ORIGIN      
        1 gtggtcgaca tgaaaaagcc ccgcattcgc ggggctttct gatgagcggc tttcgagcct
       61 cgggggtctg gcgtcagcgc ccgatgaagg ttagcgggtc gatctggcgg ccgttcttga
      121 gaacctcgaa atgcacgtgc gggccggtcg agcggcccgt ggagccgacg aaggccagga
      181 tatcgccacg ggaaaccgta cggcccacgc cgaccgccac agcctggttg tggccgtagc
      241 gcgtgatcag gccattgcca tggctgacct cgacgagggt gccgtaaccg gtcttctcac
      301 cggagaacgt aaccacacct gctgccacgg caataacttc ggaaccccgc gggctcgcga
      361 aatcgatgcc cttgtggaaa gcctggtgac cgttgaatgg atcctggcgc tcaccgaagt
      421 acgacgagat gtgaccagcc cgcaccgggc ggccatccgg gcgaatctgc tcgtcgagct
      481 tcttcgacaa cagcgcgttc tcgagcgccg ccagctgcgc gtcgcgcgag gagatcttcc
      541 gttccagccc attcaacagc gagttgaggt cgggcgcctg catggcggcg ccgatgtccg
      601 attccggacc gccgacggcc ggctggctct cgaaatcgaa ttcgcggttg tcgatgttgg
      661 ccatctgggt gaggcgcttg cccagcgcgt cgagccggat gacatgggca ttcatctgcc
      721 cgatacgcat ggctaccgcg tctacgcggc gctgaagctc ggccttgagt tccttgattt
      781 cggcctgctg ttcggcgagg gtcgcggtcc acgaagcgac cccacgcggg ggcgccttgc
      841 tctcaccgat cttgaatccg aggctgaagg cggtggccaa gatggcgacc agcacggccg
      901 caaaggcagc gacagcgaat ggatgggcca ggttgaagtg ccgggcacga ccttcacggc
      961 gcgagaagaa aatgatgttc atcgcgccag ctccaggccc tgggaacaat tcggatcttc
     1021 acgatcctgt tctttgtggt tcaaagactt gtacctcgct ggcaacctga aacaccgcaa
     1081 aaccaatagg cccagcaggc gcgaaactat gcccaaaaga ccaccccgaa acaagcaaac
     1141 cgtccgtccg ggctccaaac ccaggactgc gggagactta atctcggcgc gtctgccagc
     1201 tctcctccag cgtgcccttt cggcgcccga tgagtccgtg tggcaggtcg cagtaatgaa
     1261 ggcgctgggg ccggacctgg ccattaaggt aaatcgatgc accctggata gcggaaggat
     1321 tacggtcatc gcggagtcct ccgcctgggc cgcccgcatg cggttccagc tggcagaagc
     1381 cgagtcagag ctccgtgccg tgacgccggg atatcgcgag ctgatcgtgc gtgtccggcc
     1441 acggcaggct ggcgccagcc tgggtgcccc ccgcaagtcg tcccgataga ccacgctaca
     1501 gctcccctga ggcgagccgg tctgccgcgt agtgcgcggc gcgggccgac agggccatgt
     1561 aggtcaaaga ggggtttacg cagcccgacg acgccatgca cgaaccgtcg gtgatgaaga
     1621 ggttcggaat gtcgtgcgcc tggttgtgcc ggttgagcac ggaagtcgcg gggtcgagcc
     1681 ccattcgcgc cgtacccatt tcgtggacgc tggtgcccgg cggcgaaaga tgcgggaaca
     1741 gcgcgcggac attggtgcag cccgcggccc tgagcatcgc ctgcgcgtcc tcgtcggccc
     1801 gcgcggcgag cttgcgttcg ttctcgccga acgtgcagtc gatgtgcacc agcggcaatc
     1861 ccgaggaatc ggttctgctc tcatgcagcg tgatgcggtt gtccgcgcgc ggcaacattt
     1921 cggcgaagcc gaacaacacc atctgccagg gacccggctc gcgcaggtcg gccttcagtt
     1981 gcgcgccgac acccggactg tcgagcgcgc gatcccagtt cgggcgttcg cccttgccct
     2041 ggaacgccca gccgcgcgtg tattcaccgc gactgtcttc ggtcacgttg gcgtagcgcg
     2101 gaatgtaaat acccgtgggg cggcgcccgt agtaataacg atcgaggaag ccgggcacgt
     2161 tgcccatggc gccgatgccg tgcacgtgat ccatgaggtt gcggccgacc tggtccgagc
     2221 ggttcgcgag acccttcgga aagaattccg atttcgagtg aagcaggatc tgcgcggtcg
     2281 ggattgtcga ggcgcacagg aacaccacgc gcgcctggta gctgcgccgc tgcttcgtct
     2341 tcgcatcgat gacccgcacc gcggatatgc ggcgcttggc gtggtcgtag tcgagcgtct
     2401 cgaccactgc gtcggtgacc accgtcaggt taccggtacg ctccgcagcc ggcagtgtcg
     2461 cgctcagcgt cgagaaatat ccaccataac cgcagcctcg ttcacacacg tttctcagct
     2521 ggcagggtcc gcgcccgagc gcgatgtgtt cggcggtggg ctcagtgaga tgcgcgcagc
     2581 ggccaatcgt gacgcggcgc gacgggtggc tgctttcgag ctttcgcttg aaatcgagct
     2641 cggcgcagtt gagatccatc ggcttctgaa aacggccgtc gggcaactgc tcgagtcctt
     2701 cgctgttgcc ggaaataccg gcgaaggttt cgacgtggtc gtaccacgac gaaatgtcgg
     2761 catagcgaat cggccagtcg acgccgtggc cgtccttgct gtttgcgctg aaatcgatgt
     2821 cgctgaagcg ataggtctgc cgcgcccagg tcagcgaccg gccaccgagg tgatacccgc
     2881 gcagccagag gaactcgcga ccttcgggcg tgagatacgg atgctcgctg tccttgaccc
     2941 accattgccg ggtcgccgaa ttgaacgcgt aacaggtgct ctggatcgcg tagtcgcgac
     3001 gcacttcgtc ctcgggcacc tggccacgat gggcgagctc ccacggagcg gcgaaatcct
     3061 gatagtcggt cttgtgcgtc acgtgacgtc cacgctctat caacagcgtt cgcatcccgc
     3121 gttcgcagag ctctttcgcg gcccaaccac cgcttatccc tgagccaatg acgattgcat
     3181 cgaagtccat gatggcgtcg gagaatcagt agaacgcgga agcgcggccg acttcggtga
     3241 ccggcaggca ggcgcgccag gcgccgggaa ccagttcgaa gcgcagctcg tgcgtcgcgc
     3301 cgacctcgga gtggtaatag ccgatgagca ccagccattt gaacttgcga tatgcgccgt
     3361 catgcgcgcc gatgcgcgcg gcgtcgaatt ccttcaccac ctcgaggcgc cgttccggcg
     3421 gcatttcgag gaacgccatg ccgaaccggc tccatgcctg ctgctcgatg gcctcgagca
     3481 cattggcaat atcggttcgc gtcgcggccg atccccactg cgcgagcatc tcgcggatga
     3541 atgcgggcac gcccgcgccg atggcgccgg cagtgtcggt ggcgggaatc atcgtttcgc
     3601 tgacctgctc gagctgcgca aattgctcgg ccgaaaggac cggcccggcc gtgtcggcct
     3661 tgtcgccggt cggacgtgaa aaacgagtca gcgccgccgc cccgcccagc aggaacaccg
     3721 caccggccag caaggcacgc cgattgattt tttcgctatt acccgcttcc acgccgaagg
     3781 tcctccgccc cgatctatcg gcgacgttgc ttcgaaatga atccgcaatg tgtgcgccgc
     3841 gtcaggttcc cggggcgcat ggaaaaagga ctacagcggc ggcaaggaat tgccgcgttg
     3901 aacatcgagg gaatgctgct cgctagaagg cggagccgac cgccaccggg ggcggctgga
     3961 cgtaggcgat gggcgcgtcg gcgggcttct cgaacgtgac cgcctcccag gtggaaggct
     4021 cggaaatgag cttgcgcagc aggttgttgt tcagcttgtg gccggacttg tagccgctga
     4081 actcgccgat caggctgtgc ccgagtagat agaggtcgcc gatcgcatcg aggatcttgt
     4141 gcttgacgaa ttcgtcctcg taacgcaggc cgtcttcatt gagcacgcgg aaatcgtcga
     4201 gcacgatggc gttgtcgaag ttgccgccca gggcgagatt gcgcgcgcgc agcatctcga
     4261 ggtcgcgcat gaagccaaag gtgcgggcgc gcgacacttc cttcaggtag gaagtggtcg
     4321 agaaatccat cgaggctttc tggccgcggc gcttgaacag cggatggttg aattcgattt
     4381 cgaaattcac cttgaagcca tcgaagggcc ggaactcggc ccacttgtcg ccatcttcga
     4441 cgcgcacgcg ctgcttgatg cgcacgaatt tcttcggcgt gcgctgctct tcgaagcccg
     4501 ccgactgcag caggaacacg aaggggccgg cgctgccgtc catgatcgga acttccggcg
     4561 cgctcacgtc gacatgggca ttgtcgattc ccaggccggc gaaagccgac agcaggtgct
     4621 ccaccgtcga gacgcgggcc tcaccattaa taagagtggt gccgagctgg gtgtcgccca
     4681 cattcagcgc gtgggcgcgc acctcggcgg gcggatcgag atcggtgcgc cggaagacga
     4741 tgccggtgtc gggcgccgcg ggccgaagcg tcatcaggat cttctggccg gtatgcatac
     4801 cgacgccctg ggcgcggatg gagtttttga gagttcgttg tccgagcatc tgaaaatatc
     4861 tcccgctgcc gtttacacgg tgctcgggta gcgccgcctt cagtcactta agacgtgggc
     4921 ggacatcggg gagcggccgc aggcgcggca ccgtgccccc aaaaggggcg ctaccgtaac
     4981 acaattgcct tattcagtct ccggcatcag tcagcctgac gccgcagaaa ggccggaata
     5041 tccagcacat cttcagattc cgcgctggca tgcagaccgt cgcccaccgc tctttgacgc
     5101 tgcaccgctg gtttatccag cgctgcgtag tcggccgccg tcagcggacg gcgcggtacc
     5161 acccgcatgg agggttccgc cgccggcgct gccgcctgcg gtactgcctt cgagagtgtg
     5221 ggtacgtcgc gaccgaacgg cgaacggccg ctgaccgcga tctcgggttt gccgagcccg
     5281 gtggccacca ccgtaacccg catttcatca gacagctcgg ggtcgatgac ggttcccacc
     5341 acgacggtgg cgtcttccga agcgaactgc ttgacgatgt tgccgacttc ctggaattcg
     5401 ccgatcgaca ggtccatgcc cgcggtgacg ttcaccagga tgccgtgcgc gccggacagg
     5461 ttgatgtcct cgagcaacgg tgaagagacg gccatttcgg ccgcttcctt cgcacggttc
     5521 tcgccacgcg cgacaccgga acccatcatc gccatgccgg tctcgctcat caccgtgcgc
     5581 acgtcagcga agtccacgtt gatgaggccc ggacgcgtga tgagctcggc gatgccctgc
     5641 accgcgccct gcaacacgtg gttcgcgctc ttgaacgcgt ctaacaacgt ggtcttggcg
     5701 ccgagcaccg tgagcagctt ctggttcgga atggtaatca gcgagtcgac gtacttgccg
     5761 agttcggcca gcccgtgatt cgccaccagc gtgcg
//
LOCUS       DASQJS010000058         4638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_660026, whole genome shotgun sequence.
ACCESSION   DASQJS010000058 DASQJS010000000
VERSION     DASQJS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660026"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VEW08_02220"
     CDS             complement(<1..1111)
                     /locus_tag="VEW08_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HYJ39574.1"
                     /translation="MDSKIISWRRDFHQNPELSNRELRTSGIVADHLKKLGLEVETGI
                     AKTGVVALLRTGKPGPTIALRADMDALPVTERTDVPFKSVAKSSYRGEEVGVMHACGH
                     DSHTAILMGVAEALTRVKGSLRGNVLFVFQPAEEGAPPGEVGGASEMLKAGVFEKYKP
                     EVALGLHMWASLNTGIIGYRSGPFMAGSQAWRVVVNGKQTHGSRPWQGVDPIVVSAQI
                     INAMQTVVSRQIDITQNPAVVSAGIIKGGIRNNIIPDSAEIIGTIRTFDPKQKQQIYD
                     AMKRLVENTAEANGATATFEFDAYANPVTYNNPELTARVLPSLRKVAGAANVKEIALI
                     TGSEDFAYFGEKIPAFFFMVGITPKGTDVLTAPANH"
     gene            1286..2443
                     /locus_tag="VEW08_02225"
     CDS             1286..2443
                     /locus_tag="VEW08_02225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYJ39575.1"
                     /translation="MHVVDATLFYSPTSGGVKRYLLAKHEWMRQHTSWRHSLVVPGET
                     RSHFPGDISTVAGGLVPGAFNYRLPLNPMHWSRAIDALEPDLIEIGDAFHPAWAAASV
                     ASRRGIPLIAFYHSNFPQLAGRRLGSAIQRFIEWYVKLTYERCEQVLAPSRYMCEYLH
                     SIGVRHATCQPLGVDVDTFTPERRTRNLAAELNLPHGTRVLVFAGRFSKEKNIPVLTD
                     AFRRLGDPYHLLLIGGDETGREGNVTRIPYCRDNHALASYIASADAFVHAGTHETFGL
                     VVLEAMACGRPVVAMRAGALPELVDPGAGNLAEPHADPAVAATNLADAIVNVYERDLD
                     VLGAAARRHVLANYSWSRALQALMGRYQLAVGASRLPALANGMARAETTTQ"
     gene            complement(2460..3041)
                     /locus_tag="VEW08_02230"
     CDS             complement(2460..3041)
                     /locus_tag="VEW08_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39576.1"
                     /translation="MMVELRDLTPNERKEVQQRFELLASKRFAPSPKKPRHEVWWPKA
                     PLDGRAFPEGIRPPDGDDSFNESARLLGFLNGVCGGEFYVANLSAPKPFFRHTGKLDA
                     ELASWIRTRLVSNPTCLLASTLDVFLIVDSQMHYSVIGGPPEVIEKFEKRFGGAEALQ
                     SNFASFVDQFRVGSGLEDVKWAQDYLQKWSGWL"
     gene            complement(3038..4078)
                     /locus_tag="VEW08_02235"
     CDS             complement(3038..4078)
                     /locus_tag="VEW08_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39577.1"
                     /translation="MAAKKSLSFALEPRATASLDDYVADFAWAPDSMSFAIAGGEGKV
                     ALARAEGDALTVEVLGEHQLGALSIVWRPHANVFATSGQDSAVALWDGTSGKEIKRWK
                     PAPTPTQALSYSPDGEVLASAAGKVVTLWSAQGEKIHAFPPAASAAVALSFDRPGTDL
                     GVALNGELAVHRIEKSRYETRRYKWPAACLTVNFSGNGRFLASGMADGTLHFWNRSTG
                     KDSQMRGYDGKLELVAWSDNSRYLASSAGNEVVLWDFGGKGPEGTRPIVLSGHTERVD
                     SIAWQPGSEHLVSAGRDWRLTLWRPAKAKQPIDVQMLDSEISVVRWSPDGKRVATGEK
                     NGRVTVFELVAR"
     gene            complement(4081..>4638)
                     /locus_tag="VEW08_02240"
     CDS             complement(4081..>4638)
                     /locus_tag="VEW08_02240"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYJ39578.1"
                     /translation="TALEAAIEPAVRAFAEAATLIPCHGTLDAAPSHNAIDISHPGAD
                     FRLTIDKAGAYALFCEHHPLEFGMHLAGQRPAAERKFASHHHEDDISSIGISDPRALD
                     PVKVNQWFDYLLKTRGQDIFRMKGVLNVKGEDRRQVFHGVHMMFDAQAERPWGDAQRT
                     NSLVFIGRGLDRAELEAGFESAVAK"
BASE COUNT          808 a         1495 c         1442 g          893 t
ORIGIN      
        1 agtggttagc cggcgcggtg agcacatccg tgcctttggg cgtgatgccc accatgaaga
       61 agaacgccgg aatcttctcg ccgaaatacg cgaaatcttc cgaacccgtg atgagcgcga
      121 tctccttcac attggccgcg cccgcgactt tccgcaggct gggcagcacg cgcgcggtga
      181 gttcggggtt gttgtaggtc acggggttcg catacgcatc gaactcgaac gtggccgtgg
      241 cgccattcgc ttcggcggtg ttctccacga gccgcttcat ggcgtcgtag atctgctgct
      301 tctgcttggg atcgaaagta cggatcgttc cgatgatctc ggcggagtcc gggatgatgt
      361 tgttgcggat gccgcccttg atgatgcccg cggagacgac ggcgggattc tgcgtgatgt
      421 cgatctgccg gctcacgacg gtctgcatgg cgttgatgat ctgcgcggag acgacgatcg
      481 gatccacgcc ctgccaggga cgcgagccat gtgtctgctt gccgtttacc accactctcc
      541 aggcctgcga acccgccatg aacgggccgc tgcgatagcc aatgattccg gtgttcaacg
      601 aagcccacat gtgcagcccc agggctacct cgggtttgta cttctcgaag acgccagcct
      661 tgagcatctc ggatgcgcca ccgacttcgc cgggcggcgc cccctcttcc gcaggctgga
      721 acacgaacag cacgttgcca cgcagcgagc ccttcaccct ggtcagagcc tcggcaacgc
      781 ccatgaggat ggccgtatgc gagtcgtgcc cgcaggcatg catgacgcca acttcttcgc
      841 cccggtagct agacttcgcc accgacttga acggtacgtc ggtgcgctcg gtgacgggca
      901 acgcatccat gtcggcgcgc agcgcaatcg tgggcccggg tttgccggtg cgcagcaatg
      961 ccaccacgcc ggttttcgcg atgcctgtct ccacttcgag acccagtttc ttcagatgat
     1021 ccgcgacgat ccctgaggtg cgaagttcgc ggttggaaag ttccggattc tgatgaaaat
     1081 cccgtctcca gctgatgatc ttgctgtcca ccgcctttat ttcggcttcg agatctttgg
     1141 agtaggctgg tgaaagccaa aaggctgcta gtgctaccca aggtagcaat ttgtaggtgt
     1201 aagtcgacat agggaaccga gtctgcgctg actggtcgca gtactgcaac gccccgatac
     1261 ttcgcaagag caagaagaat cactcatgca tgtagttgat gccacgctct tctattcgcc
     1321 taccagcggc ggagtgaagc gctacctgtt agcaaaacac gaatggatgc gccagcacac
     1381 cagctggcgg cacagcctcg tggtgccagg agagactcgc tcgcattttc ctggcgacat
     1441 ctcgacggtt gccggcgggc tcgtaccggg tgccttcaac tatcgcctgc cgttgaaccc
     1501 catgcactgg tcgcgcgcca tcgacgcact cgaaccggat ctcatcgaga tcggcgacgc
     1561 attccacccg gcgtgggcgg cggccagcgt cgccagtcgc cgcggcatcc cgctcatcgc
     1621 cttctaccat tcgaactttc cgcagctcgc gggccggcgg ctcggcagcg cgatacaacg
     1681 tttcatcgag tggtacgtaa agctcaccta tgagcgttgc gagcaggtgc tcgcgcccag
     1741 ccgctacatg tgcgagtacc tgcacagcat tggagtcagg cacgccacgt gtcagccact
     1801 gggcgtcgac gtcgatacct tcacacccga acgacgcact cgcaatctcg ccgccgagct
     1861 caacctgccg cacggcacgc gagtcctggt gttcgcggga cgcttctcga aggagaaaaa
     1921 cattccggtg ctcaccgatg cgtttcgacg gcttggcgac ccgtatcacc tgctcttgat
     1981 aggcggcgac gaaacgggcc gcgagggaaa tgtcacgcgc atcccttatt gccgcgacaa
     2041 ccacgcgctg gccagctaca tcgcttcggc cgatgcattc gtgcacgccg gcacgcatga
     2101 aaccttcggg ctcgtcgtcc tggaagccat ggcctgcggt cgcccggtcg tggcaatgcg
     2161 cgccggggcg ctgccggagc tcgtcgaccc gggcgcgggg aacctcgccg agccgcacgc
     2221 ggatccggca gttgcggcca cgaatctcgc cgacgccatc gtcaatgtgt atgaacgcga
     2281 cctcgatgtg ctcggcgccg ccgcgcgccg ccacgtgctg gccaactaca gttggtcgcg
     2341 ggcgctacag gctctcatgg gtcgttacca gctcgcggtc ggcgcaagcc gcctgccggc
     2401 gctggccaat ggcatggcgc gcgccgagac gactacgcaa tagccaaggc tgacggctgt
     2461 cagagccagc cggaccattt ctgtagatag tcctgcgccc acttcacgtc ttcgagacct
     2521 gagccaacgc gaaactggtc gacgaagctg gcgaagttgc tctgcaacgc ctcggctccc
     2581 ccgaaacgtt tttcgaactt ctcgatgact tcgggcgggc cgccgatcac ggagtagtgc
     2641 atctgcgagt cgacgatcag gaatacgtcg agcgtcgatg cgagcaggca ggtggggttc
     2701 gacaccaggc gcgtcctgat ccagcttgcg agctcggcgt cgagttttcc ggtgtggcgg
     2761 aaaaatggct tcggcgcact caggttcgcc acgtagaact cgccgccgca aacgccgttg
     2821 agaaaaccca gcaggcgcgc ggattcgttg aagctgtcgt cgccgtcggg cggtcgtatg
     2881 ccctcgggaa atgcgcgccc gtcgagcgga gccttgggcc accacacttc gtgtcgaggt
     2941 ttcttcggcg acggagcgaa acgcttgctg gcgagcagct cgaagcgctg ctgcacttcc
     3001 ttgcgttcat tcggtgtgag gtcacgcagc tcgaccatca tcgggccacc agctcgaaca
     3061 cggtgacgcg gccgttcttt tcaccggtgg ccacgcgctt gccgtcgggg gaccagcgca
     3121 ccacgctgat ctccgaatcg agcatctgca cgtcgatggg ttgtttggcc ttggcgggcc
     3181 gccacagcgt gagccgccag tcgcgtcccg cgctgaccaa gtgctcgctg cccggctgcc
     3241 aggcgatgct gtcgacgcgt tcggtatggc cgctcaacac gatggggcgc gtgccttccg
     3301 gacccttgcc gccgaaatcc cacagcacga cctcgttgcc ggcgctcgaa gcgagatagc
     3361 gactgttgtc gctccacgcc accagttcga gcttgccgtc atagcctcgc atctgtgaat
     3421 ccttgcccgt tgaacgattc cagaagtgca gcgtgccgtc ggccatgccg ctagcaagga
     3481 atcgtccgtt gccgctgaag tttaccgtga gacaggcggc cggccacttg tagcgccgtg
     3541 tttcgtagcg cgatttctcg atgcgatgca ccgcgagttc gccgttcagg gcaaccccga
     3601 gatctgttcc aggccgatcg aacgacagcg ccaccgcggc gctcgcggcg ggtgggaacg
     3661 catggatctt ttcgccctgg gctgaccaga gcgtcaccac tttgccggcc gcgctcgcca
     3721 ggacctcgcc atcgggcgaa tagctcaggg cctgggtcgg cgtgggcgcg ggcttccagc
     3781 gcttgatctc tttgcccgac gtaccgtccc agagcgccac cgcgctgtcc tggccggaag
     3841 tcgcgaacac gttcgcgtgc ggacgccaca cgatgctcaa cgcgcctaac tggtgttcgc
     3901 cgagcacttc gaccgtcaac gcatcgcctt cggcgcgcgc cagcgcgacc ttgccctcac
     3961 cgccggcgat cgcgaacgac atgctgtcgg gtgcccacgc gaaatcggcc acgtagtcgt
     4021 cgaggctggc ggtcgcgcgt ggctccagcg caaatgacag ggacttcttc gcggccatga
     4081 ttatttcgcg acggcggatt caaatccggc ttcgagctcg gcacggtcga gtccacggcc
     4141 gatgaacacg agactattgg tgcgttgggc gtcgccccag ggtcgttccg cctgcgcatc
     4201 gaacatcatg tgcacgccgt gaaatacctg gcggcgatct tcacccttca cgttcagcac
     4261 gcctttcatg cgaaagatgt cctgcccacg ggtcttgaga agatagtcga accactgatt
     4321 gaccttcacc ggatcgagcg cacgcggatc gctgatgccg atggagctga tgtcgtcctc
     4381 gtgatgatgc gaggcgaact ttcgctcggc ggcgggccgc tggccggcga ggtgcatccc
     4441 gaactccagc ggatgatgct cgcagaacag cgcataggcg ccggccttgt cgatggtcag
     4501 cctgaagtcc gcgccgggat gcgagatgtc gatggcgttg tgcgacggcg ctgcgtcgag
     4561 cgtgccgtgg caggggatga gagtcgcggc ttccgcaaac gcgcgcacag cgggttcgat
     4621 ggctgcttcg agtgccgt
//
LOCUS       DASQJS010000059         9022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_344907, whole genome shotgun sequence.
ACCESSION   DASQJS010000059 DASQJS010000000
VERSION     DASQJS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9022
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344907"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            753..1622
                     /locus_tag="VEW08_02245"
     CDS             753..1622
                     /locus_tag="VEW08_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HYJ39579.1"
                     /translation="MNAPGGTEEVDFKSSSGYLALLFMFIALLAIPLSVMARFPPVIL
                     LAVLTLIFLAKGMYMLQPNQSALMMLFGAYRGTDYSTGLRWANPFLNKKKVSLRLRNF
                     NSEKLKVNDKRGNPIEIAAAVVWKVKDTARAVLDIDHYEHYVPVQAESAVRHLANEYS
                     YDAHDDDQAELTLRGGGDEIVMRLKRELQERFEKAGLHVEDARLTHLAYAPEIAGAML
                     RRQQAEAVIGARKQIVLGAVSMVEMALKGLQERGVVELDDERKAAMVSNLLVVLCAES
                     EAQPVINAGSLYQ"
     gene            1635..1838
                     /locus_tag="VEW08_02250"
     CDS             1635..1838
                     /locus_tag="VEW08_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008782442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HYJ39580.1"
                     /translation="MSEPPARKAFLLRLDPAVAAAIEAMAAQELRSVNGQIEWLLREA
                     LAKRGRAINTMSRTNARRPRKDE"
     gene            complement(1853..3469)
                     /locus_tag="VEW08_02255"
     CDS             complement(1853..3469)
                     /locus_tag="VEW08_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HYJ39581.1"
                     /translation="MRHGILMTAILFSAGLLSACGQGDAASAPKPAPKAAEDVSLASV
                     HADAPGIAWFNGDVEAAFASAKASNKPVLLYWGAQWCPPCKQLKSAVFNRPDFIEKSK
                     LFVAVYLDGDLPDAQKWGDEFRVTGYPTVVVFKADRTEITRIAGNMDLSLYAGVLDNA
                     LGDVRPVKDVIELAVKGEAPLAADDCRRLAYHAFGLEDDGVFAAAKLQTAFENAARTC
                     PAELTRERARLHIRAAQQATEQQKDVLKSGGKADKALTVSIVRVNELLTNKPLALANA
                     DALRGLRTEFFLAARQTLPQLAPTLRERWMAVADAATANAEFAPADQLAAQLMKITAA
                     KAYAADSKVPTDVRSVALATADKMLAVKQDAYVRAGVVNSAINIYIALDDWERARDLL
                     TLEATTSNTPYYYLGDLADVEEHLGNIDRSVELLAEAYAKAKGPASRFQWGYNYLDGL
                     LRMKPDDLATIEKVGAQVIAELDGPNRIHRRTLSRLNKLDEKLNEWNTSAARGKVATN
                     LRAGVVRACEKSNGGKGGNCISVASAAAAT"
     gene            3525..5534
                     /locus_tag="VEW08_02260"
     CDS             3525..5534
                     /locus_tag="VEW08_02260"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HYJ39582.1"
                     /translation="MKRPSFESVLSTRLSRRTVVAGAAATVGLAACAQIPTKRETALK
                     TSKGPSIFNSIAPQNLDVFAVADGYRYNIIARWGDSIVTGTSDFDTRRLTDTSWLDAS
                     AAATQERQFGTNADAVAYFPQSSGRAARGLVCVNHEYVNGELMFAGHRGASMKFEARK
                     AWLDKHPNAIAVVQAAHGVTVMQLHRDSQGWTRNLAARENRRITARTPMDIHGPARGH
                     ELMRTNADPGGTRVLGTFANCSAGKTPWGTYLTSEENVDDYFAGGGTVRESSKDAAML
                     DANRRFALREYSFYGWDHQDPRFDVRIEPHEPFRFGWMVEIDPHDPKSIPRKRTSLGR
                     FQHEGANTIVARSGHVVAYMGDDEKFEYIYKFVTRDRFNPKNPAANRDLLDHGTLYCA
                     RYDADGTGEWLPLVHDENGPLNSRAGFANQGDVVIKVRAAADLLGATPMDRPEDVEPS
                     PVTGRIYIPCTKTSERGNSEEREWFGRKVDVAPDAANPRPDNKSGHIIEMAEAGDDAT
                     GTRFEWNVFLLAGNPAEGRYIVDARDLAAGSLSGTDTYFAGYPNRAEQSQVHCPDNLG
                     IDPQGRLWIVTDSDNRGNPNNGCFVVPTSGPQRGLLKQLASGPTGCELCGCEFTPDGR
                     TLFLTIQHPGEGGSLDNPRSHWPDGNGMPARAAILAIEREDGGTV"
     gene            5599..6162
                     /locus_tag="VEW08_02265"
     CDS             5599..6162
                     /locus_tag="VEW08_02265"
                     /inference="COORDINATES: protein motif:HMM:NF022426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HYJ39583.1"
                     /translation="MNTRSLLRVFAALAVLGSLGGCASLSKSECMNASWEDIGIRDGA
                     NGQPEEYLIQHSTACAKVNVVPDRGAWLHGRETGLERFCVPHRAYQIGEYGNGFDVGI
                     CRNFDQERLQDAYNKGRDVHQRSNELGSIDSELRDINIRLEDKDKEHPLTKKERDALM
                     FRLGALTVQRVNAQRAYDDARYRARDL"
     gene            complement(6175..6927)
                     /locus_tag="VEW08_02270"
     CDS             complement(6175..6927)
                     /locus_tag="VEW08_02270"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HYJ39584.1"
                     /translation="MDAKPKSAHGGLRDRLPLAPRREQNFRWRGNEVTRVEGFTDAVF
                     AFAVTLLVVALEVPHTFEGLMDVVRGFPAFVICFTLLMTFWNAHYRYHRRYGIESVFS
                     RVMTMAIIVLVLFFVYPLKYLFTLLTVQIFALDLHNAPHLDGWYQVQTVYLIYGLGLG
                     GVWGLYAALYGHALANREKLQLDAIETALTRADLRSNLIYVVVCCLSMLLALVTSSVW
                     LPGVIYFLLGPLQTANGIWSGRQVQRLAAPAA"
     gene            complement(6971..>9022)
                     /gene="hrpB"
                     /locus_tag="VEW08_02275"
     CDS             complement(6971..>9022)
                     /gene="hrpB"
                     /locus_tag="VEW08_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HYJ39585.1"
                     /translation="VMSATLDGAAVARLLGNAPVVTAAGRVFPVEIQYVGSGMPMLPG
                     GRDAPELAVVRTIKRALADAPGDMLVFLPGAGEIRRVQGMLAGVGNDVDVMPLFGELA
                     AGDQDAALRPAKAGRRKIVLATNIAETSLTIDGVRIVVDAGLERRSLFDPASGMNRLE
                     VQRISRASAEQRAGRAGRTAPGVCFRLWGEGAERSLAAFAPPEVCVADLAPLALDLAA
                     WGTEANSLRWLDAPPAATLASARDLLRRLGALDGAGKVTAHGRAMQEFPAHPRLAHML
                     LKARELQAGSLAAELAALLSDRDLLRAGAGGNPRARDSDIRTRLEALRRGSADIDRGA
                     LDRVRRTQRSFEQQLGAAGVSRGGNVDPGVLLGLAYPDRVARRRPGSEARYQMSSGRG
                     ALFAAAESIAREEFIVAVDLDDREREAQIRLAAPLTKAHLLEYFAPDIRRADELAWDE
                     RLEAVVARRVIRFDELLVEEKPLNDVPRAASAAAMLEGVRSMGIEALPWDDDARDFQA
                     RCEFVRKLDRKDLGDWPDFSPPALAQDLGWLEPFLEGATRRTQLNRVSVHSALRARLT
                     YEQQRKLDSLAPTHIDLPTGTHTRIDYLDDNAPVASMRMQEVFGLAATPRIGGGTVAV
                     TFTLLSPGRKPLQITRDLASFWRNAYVEVRKDMRGRYPRHYWPENPLEAEPTRRAKPR
                     A"
BASE COUNT         1682 a         2957 c         2815 g         1568 t
ORIGIN      
        1 ggagaaacta gttggtcagc gttatgcggc aatgcctgat gcattggcca ccaaatttcc
       61 gcgggcggca ctgagccttt cgtggcaatt cttgttcggc tccgcgactc gctgcttcaa
      121 tgcggcccgc aatcgatggg agcgttggca tgcatcacca accggcttgc agcgcgcctt
      181 ccacatagct gtggctcgag gggcgcgctg ccccacatga tcaatctatt cctaccagta
      241 ggcatagttt gatcaaactg atcaaactat ggttccacgc gcacacggcg ctttcccgat
      301 ggtatagatg gtgggcctgc ttggattcga accaaggacc aagggattca cgttgccgaa
      361 gcgtttccgc cccgcgtgga ctatctcttc acccgcgagc agttatctct aactacccgg
      421 tagggtgcgg gacgctctag cctgtcatca agggcgcttc gagccctcag gtagtctctg
      481 caccttccga agctgccgcc tcggcttggc tcagggttgc catgcgcgcc ttcttccatg
      541 aagaaggggc acgaaggttt ccctgaattc atcccgtcca ctgcaccggt ttcccggtgc
      601 aggcacctat atagatgagt cccctgcact aaccgctgtg ctacaggccc gaccgggcgc
      661 gattgtacag gggaaggtca aatcgggctt gtgtgtaaag tgatatcact ttagtatcaa
      721 cttcagttga taaccaagag agggagaaag tcatgaatgc gcctggtggt acggaagaag
      781 tcgatttcaa gagctccagt ggctacctgg ccctgttgtt catgttcatc gcactgctcg
      841 cgattccgct gtccgtcatg gcgcgattcc cgccggtgat tttgctcgca gtgctcaccc
      901 tgatttttct cgcgaagggc atgtacatgc tgcagccgaa ccagtcggcg ctgatgatgc
      961 tgttcggcgc ctatcgcggc acggactatt ccaccggcct gcgctgggcg aaccccttcc
     1021 tgaacaagaa gaaggtctcg ctgcgcctgc gcaacttcaa cagcgagaag ctgaaggtga
     1081 acgacaagcg cggcaacccc atcgagatcg ccgcggctgt cgtgtggaaa gtgaaagaca
     1141 cggcgcgcgc ggtgctcgac atcgatcact acgagcacta cgtgcccgtg caggcggagt
     1201 cggcggttcg ccacctggcc aatgaatact cctacgacgc gcatgacgac gatcaggcag
     1261 agctcacgct gcgcggcggc ggagacgaaa ttgtcatgcg tctcaagcgc gaactgcagg
     1321 agcgcttcga gaaggccggg cttcacgtcg aagacgcgcg cctcacgcac ctcgcctacg
     1381 cgcctgaaat cgcgggcgcc atgctgcggc gtcagcaggc cgaagcggtc atcggcgccc
     1441 gcaagcagat cgtgctcggc gcggtcagca tggtcgagat ggcgctcaag ggcctgcagg
     1501 aacgcggcgt ggtggagctc gatgacgagc gcaaagccgc catggtctcg aacttgttag
     1561 tggtgctgtg cgcggaaagt gaggcgcagc ccgtgatcaa tgccggctcg ctgtatcaat
     1621 aacgcggtgc cgtcttgagc gagccgccag cacgaaaagc gttcctgctg cgcctcgatc
     1681 cggcggttgc cgccgcgatc gaggcgatgg cggcgcagga gctgcgcagc gtcaatggcc
     1741 agatcgagtg gttactgcgc gaagcgctgg ccaagcgcgg tcgcgcgatc aataccatgt
     1801 cgcgaaccaa cgcccgcagg ccgcgaaagg acgaataaca aagacgagtg aatcaggtag
     1861 cggccgcggc ggaagccact gaaatgcaat tgccgccctt gcccccgttc gatttctcgc
     1921 aggcgcgcac gacgcccgca cgcagattgg tcgccacctt ccctcgcgcg gccgaggtgt
     1981 tccactcgtt gagcttctcg tcgagcttgt tgaggcgcga tagcgtgcgg cggtgaatgc
     2041 gattcggtcc gtcgagctcg gcgatgacct gggcccccac cttctcgata gtggcgagat
     2101 cgtccggctt catgcgcaac aggccatcga ggtagttgta gccccactgg aatcgggacg
     2161 cgggtccctt ggccttggcg tacgcctcgg ccagcagctc cacgctgcga tcgatgttac
     2221 cgaggtgctc ctcgacatcc gcgaggtcgc ccaggtagta gtaaggcgtg ttcgacgtcg
     2281 tcgcctcgag cgtcagcaga tcgcgggcgc gttcccaatc gtcgagcgcg atatagatgt
     2341 tgatggcgga gttcacgacg ccggcgcgca cataggcgtc ctgcttcacg gcgagcatct
     2401 tgtccgcggt ggcgagtgcg acgctgcgta cgtcggtcgg aaccttgctg tcggcggcat
     2461 aagccttggc cgcggtgatc ttcatgagtt gcgcggccag ctggtccgca ggcgcgaatt
     2521 ccgcgttggc agtggcggca tcggcgaccg ccatccagcg ctctcgcaac gtgggcgcga
     2581 gctgcggcag cgtctggcgc gcggcgagga aaaactccgt gcgcaatcca cgcagcgcat
     2641 ccgcgttggc gagcgcgagt ggcttgttgg tcagcagctc attgacgcgc acgatggaca
     2701 cggtgagcgc cttgtcagcc ttgccgccgc tcttgagcac gtctttctgc tgctccgtgg
     2761 cctgctgcgc ggcgcgaatg tgcagacgtg cgcgttcccg ggtcagctcg gccgggcaag
     2821 tgcgggccgc gttctcgaac gctgtctgca gcttcgcggc cgcaaaaaca ccgtcatctt
     2881 cgagcccgaa cgcgtgatat gcgaggcggc ggcaatcatc ggcggcgaga ggcgcctcgc
     2941 ccttcactgc cagctcgatg acatccttga cgggtcgcac gtcgccgagc gcgttgtcga
     3001 gcacgccggc atacagcgag aggtccatgt tgccggctat acgtgtgatc tcggtgcggt
     3061 ccgccttgaa caccaccacc gtgggataac ccgtgacccg gaattcatcg ccccacttct
     3121 gcgcatcggg caggtcgccg tcgagataga ccgcgacgaa cagcttcgac ttctcgatga
     3181 agtctggccg gttgaacacc gccgacttga gctgcttgca tggcgggcac cactgcgcgc
     3241 cccagtacag cagcacaggc ttgttagacg ccttggcgga agcgaatgcg gcttcgacgt
     3301 cgccattgaa ccaggcgatg ccgggcgcat cggcgtgcac gctggcgagg ctgacatcct
     3361 cggcggcttt cggcgcgggc tttggcgccg aggcggcatc gccttgcccg caggcgctga
     3421 gcaagccggc ggaaaacaga atcgcagtca tcaaaatgcc atgacgcatt tgtaatcaat
     3481 ccttacctag gcggcgaaat ggggccacgt tagcatgcgg tacgatgaaa cgacccagct
     3541 ttgaatccgt gctttccaca agactttctc gccggactgt cgtcgcgggc gctgccgcta
     3601 ccgttggact cgccgcctgt gcgcagattc ccacaaagcg cgaaacggcc ctgaaaacga
     3661 gcaagggtcc cagcattttc aacagtatcg cgccgcaaaa cctcgacgtt ttcgccgtgg
     3721 ccgacggcta tcgctacaac atcatcgccc gctggggtga cagcattgtc accggcacgt
     3781 ccgatttcga cacccgccgc ctcactgaca ccagctggct ggacgccagt gcggcggcaa
     3841 cccaggaacg gcagttcggc accaacgccg atgccgtggc gtattttccg caatcctcgg
     3901 gccgggccgc acgtggcctg gtgtgcgtga accacgaata tgtgaatggc gaactcatgt
     3961 tcgccggcca tcgcggtgcc tcgatgaaat tcgaggcgcg caaggcctgg ctcgacaagc
     4021 atccgaacgc gatcgcggtc gtacaggcgg cgcatggcgt cacggtcatg caactgcacc
     4081 gggattcgca aggctggaca cgcaacctcg ccgcacgtga aaaccgccgc atcaccgcgc
     4141 gtacgcccat ggacatacat ggcccggcgc gcggccacga gctcatgcgc acgaatgccg
     4201 atccgggcgg tacgcgcgtg ctcggcactt ttgcgaactg ctccgcgggc aagacgccct
     4261 ggggcactta cctcacctcc gaagaaaacg tcgacgacta ttttgcgggc ggaggcacgg
     4321 tgcgcgaatc cagcaaggat gccgcgatgc tcgacgccaa tcgccgcttc gcgctgcgtg
     4381 agtacagctt ctatggctgg gaccaccagg atccgcgttt cgatgttcgt atcgaacccc
     4441 acgagccatt ccggttcggc tggatggtgg aaatcgaccc gcacgatccg aagtccattc
     4501 cacgcaaacg cacttcgctc ggacgctttc agcacgaagg cgcgaacacc atcgtcgcca
     4561 ggagcggcca cgtcgtggcc tacatgggcg acgacgaaaa attcgaatac atctacaagt
     4621 tcgtcacgcg cgaccgtttc aatccgaaga acccggccgc caaccgcgac ctgctcgatc
     4681 acggcacgct gtactgcgcg cgctacgacg cggatggcac gggcgaatgg ctgccgctgg
     4741 ttcacgacga aaacggcccg ctgaattccc gcgccggctt tgccaaccag ggcgatgtcg
     4801 tcataaaggt gcgcgccgcc gcggacttgt taggcgccac accgatggat cggccggaag
     4861 acgtggagcc cagccccgtc accggccgca tctacattcc ctgcaccaaa acctctgagc
     4921 gtggcaattc ggaagagcgt gagtggtttg gccgcaaggt cgacgtggcg ccagatgccg
     4981 cgaatccgcg tcccgacaac aagtccggcc acatcatcga aatggccgag gccggcgatg
     5041 acgcgacggg cacccgcttc gagtggaatg tgttcctgct cgccggcaat ccggcggagg
     5101 gccgctacat cgtcgatgca cgcgacctgg ctgccggtag tttgtccggc accgatacct
     5161 acttcgcggg ttaccccaac cgcgccgaac agagccaggt ccactgccct gacaatctcg
     5221 gcatcgatcc gcaaggacgc ctgtggatcg tcactgactc cgacaatcgc gggaacccca
     5281 acaacggctg cttcgtcgtg cccacgagcg ggccgcaacg cggcctgctc aagcagctcg
     5341 cgagcgggcc cacgggctgc gaactctgcg gctgcgaatt cacgcccgat ggccgcacgc
     5401 tgtttctgac catccagcac ccgggcgaag gcggcagcct cgacaacccg cgcagccact
     5461 ggccagatgg caacggaatg cccgcccgcg ccgcaatatt ggccatcgag cgcgaggatg
     5521 gcggcacggt ttaacattgt tagcagaacg tgaagcccgt tcaggtgcga ttcaattcgc
     5581 ctgttctaga gtttgcccat gaacaccagg tctctcctac gggttttcgc ggcgctggca
     5641 gtgctcggct cgctcggcgg ctgcgccagt ctttccaaga gcgaatgcat gaacgccagc
     5701 tgggaagaca tcggcattcg cgacggcgcg aacggccagc ccgaggagta cctcatccag
     5761 cattccacgg cctgcgcgaa agtgaacgtg gttccggatc gcggcgcctg gctccatggc
     5821 cgggaaaccg gactcgagcg tttctgcgta ccgcatcgcg cctaccagat cggcgaatac
     5881 ggcaatggct tcgacgtcgg catctgtcgc aacttcgatc aggagcggct gcaggatgcc
     5941 tacaacaaag gccgcgatgt gcaccagcgc agcaacgagc tgggctccat cgacagcgag
     6001 cttcgcgaca tcaatatccg gctggaagac aaggacaagg aacatccgct caccaagaag
     6061 gagcgcgatg cgctgatgtt ccggcttggc gctctcaccg tgcagcgagt caacgcccag
     6121 cgggcttacg acgatgcgcg ttaccgcgcc cgggacctct gagccggttc gccgctacgc
     6181 ggcgggcgct gccagccgct gcacctgcct gcctgaccag attccattcg ccgtctgcag
     6241 tggaccgagc aggaagtaga tgaccccggg cagccagaca ctggacgtta ccagtgccag
     6301 cagcatcgac aggcagcaaa caaccacgta gatgagattg ctgcgcaggt cggcgcgagt
     6361 cagcgccgtt tcgatggcat cgagctgcag tttctcgcgg ttcgcgagcg cgtggccata
     6421 gagcgcggcg tacagacccc acactcctcc aagccccaat ccataaatga ggtacaccgt
     6481 ctgcacctgg taccagccgt ccaggtgtgg cgcgttgtga agatcgagcg cgaatatctg
     6541 cacggtcagc agcgtgaaca ggtacttgag tggatagacg aagaacaaca ccagcacgat
     6601 gatggccatg gtcatcacgc gcgaaaatac gctctcgatg ccgtagcggc ggtgatagcg
     6661 gtagtgcgcg ttccagaatg tcatgagcaa cgtgaagcag atgacgaacg cgggaaatcc
     6721 acgcaccacg tccatcagcc cttcgaaggt gtgcggcacc tcgagcgcga ccaccagcag
     6781 tgtcaccgca aaggcgaaga ctgcgtccgt gaatccctcg acacgcgtga cttcgttgcc
     6841 gcgccagcgg aagttttgct cgcggcgcgg cgccagtggc agccgatcac gcagaccgcc
     6901 atgtgcagac ttcggtttgg catccattga cgaccccctg atgtcctaac agcttagcgc
     6961 aaaccggtgg ctaggcgcgc ggcttcgcgc gccgcgtcgg ctcggcctcc aacggatttt
     7021 ccggccagta gtgcctcgga tatcttcccc gcatgtcctt gcgcacctcg acgtaggcgt
     7081 ttcgccagaa gctggcgagg tcgcgcgtga tctgcagggg tttacgtccc ggagacagca
     7141 gcgtgaaggt caccgccacc gtaccgccgc cgatgcgggg cgtggccgcc agcccaaaga
     7201 cttcctgcat gcgcatcgac gccactggcg cgttgtcatc caggtagtcg atgcgcgtgt
     7261 gcgttcccgt gggcagatcg atgtgcgtgg gtgcgagcga atcgagcttg cgctgctgtt
     7321 cgtaggtcag ccgcgcgcgc agcgcactgt gcacggacac gcgattgagc tgtgtacgac
     7381 gggtcgcccc ctcgagaaac ggttccagcc agcccaggtc ctgcgccagc gccggcggcg
     7441 aaaagtcggg ccagtcgccc aggtccttgc gatcgagctt gcgtacgaac tcgcagcgcg
     7501 cctggaagtc gcgcgcgtcg tcgtcccagg gcaaagcttc gatgcccatc gagcgcacgc
     7561 cctcgagcat cgcggcggcg ctggcggcgc gcggcacatc attgagcggc ttttcctcga
     7621 ccagcagctc atcgaagcgg atcacgcgtc gcgctaccac ggcttccagg cgttcgtccc
     7681 aggcgagctc atcggcacgc ctaatgtccg gcgcgaagta ttcgagcaga tgggcctttg
     7741 ttaacggcgc agccaggcga atctgcgctt cacgctcccg atcgtcgagg tcgacggcga
     7801 cgatgaattc ttcgcgcgcg atcgactccg cggcggcaaa caacgcgccg cgcccgctgc
     7861 tcatctggta acgcgcttcg ctaccgggcc tgcgccgcgc gacgcgatcc ggataggcaa
     7921 ggcctaacaa cacacccgga tcgacgtttc caccgcgcga cactcctgcg gcacccagtt
     7981 gctgctcgaa cgaacgctgc gtgcgccgca cgcgatcgag cgcgccgcga tcgatgtccg
     8041 ccgagccacg acgcagcgcc tcgagccggg tgcggatgtc actatcgcgc gcgcgcggat
     8101 tgccgcctgc tcccgcgcgc agcagatcgc gatcggacaa cagcgccgcg agctccgcgg
     8161 ccagcgagcc ggcctggagc tcacgcgcct tcagcagcat gtgcgcaagc cgcgggtgcg
     8221 cgggaaattc ctgcatggcg cggccgtgcg cggtcacttt gccggcgccg tccaacgcgc
     8281 caagccggcg cagcaggtcg cgtgcgctgg cgagcgttgc ggcgggcggc gcgtcgagcc
     8341 agcgcaggga attcgcttcc gttccccacg ccgcgaggtc cagcgccagt ggcgcgaggt
     8401 cggcaacgca cacttcgggc ggcgcaaatg cggcgagact tcgctccgcg ccttcgcccc
     8461 acagccggaa gcacacaccg ggcgcggtgc ggcccgcacg gcccgcgcgt tgttccgccg
     8521 aagcgcgcga aatgcgctgc acttccaggc gattcatgcc gctcgccgga tcaaacaggc
     8581 tgcgtcgctc caggcccgca tccacgacga tgcgcacgcc atcgatggtg agactggttt
     8641 cggcgatgtt ggtcgccagc acgatcttgc ggcgccctgc ttttgcgggc cgcagtgctg
     8701 cgtcctggtc gccggccgcc agttcgccga acaacggcat cacgtccacg tcattgccaa
     8761 cgccggccag cattccctgc acgcggcgga tctctccggc gccgggcagg aacaccagca
     8821 tgtcccccgg tgcatcggcc agcgcccgct tgatcgtgcg cacgacggcg agctccgggg
     8881 catcgcggcc ccccggcagc atcggcatgc cgcttcccac gtactgaatc tcgaccggaa
     8941 acacgcggcc cgcagccgtg accaccggcg cgttgcctaa caacctggcg accgcggcgc
     9001 catccagtgt cgcggacatc ac
//
LOCUS       DASQJS010000060        17093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_974123, whole genome shotgun sequence.
ACCESSION   DASQJS010000060 DASQJS010000000
VERSION     DASQJS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17093
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974123"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /gene="icd"
                     /locus_tag="VEW08_02280"
     CDS             complement(<1..966)
                     /gene="icd"
                     /locus_tag="VEW08_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019689834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HYJ39586.1"
                     /translation="MQYEKIAVPKDGQRITINPDSSLNVPENPIIAFIEGDGIGIDIT
                     PVMLKVVNAAVEKAYGSRRRISWVEVFAGEKAAKVYGTGFPDESLTALRDFVVSIKGP
                     LGTPVGGGMRSLNVAMRQNLDLYACVRPIRYFPGVSTPMADASLTDMVVFRENTEDIY
                     AGIEWPTGSQEVHKLIHFLQSELKATGIRFPASSGIGIKPVSKEGSQRLVRKAIQYAI
                     ENDRVSVSLVHKGNIMKYTEGAFMQWGYLLAKEEYGAQPIGAGPWCSFKNPINGKEIV
                     VKDVIADNFLQQVLMRPEDYDVIATLNLNGDYISDALAAQVGGIGI"
     gene            complement(984..2339)
                     /locus_tag="VEW08_02285"
     CDS             complement(984..2339)
                     /locus_tag="VEW08_02285"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HYJ39587.1"
                     /translation="MRTPDTTPWHPASWTSRTAAQQPRYNDAAALETAVASLSRLPPL
                     VVSWEIEQLRERLAEAQIGRKFLLMGGDCAETFEECESDKIAKKLKILLQMSLVLLHG
                     LKKPIIRVGRMAGQYAKPRSADTETRNGITLPAFRGDLVNRSEFTAVAREPDPTLLLR
                     GYERAALTLNFVRALCDGGFADLHHPRYWDLGFMKHSPLVAQYQKIVDEIGDSLDFFE
                     RVSGGRAPHEVTAVDFFAAHEGLLMPYEQAQTRYIERQKRWYNLGTHMPWIGMRTAAL
                     DGAHVEFFRGISNPMGVKIGPAMTTEWLQGLVTTLNPQNTPGRLTIIHRFGAKDVEKG
                     LPQMIKAVRETGQQVLWVCDPMHGNTETSSGGLKTRRFENILKELELSFQVHEANGSF
                     LGGVHIELTGEDVTECTGGARGLTDADLARAYKSTVDPRLNYEQALEMAMLIGRHGPA
                     R"
     gene            2397..3623
                     /locus_tag="VEW08_02290"
     CDS             2397..3623
                     /locus_tag="VEW08_02290"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HYJ39588.1"
                     /translation="MHKVLILGAGKIGALISGLLAESGDYDVTLGDVDAVAAEAVVKA
                     HGSHNLRSVALDASDPKALENHFSAHKPQAVISSLPYYCNPEVAKAARKGNSHYFDLT
                     EDVAVTQGVRRLARGATKAFVPQCGLAPGFISIAANELTKHFDELRSIKLRVGALPQH
                     PNNVLKYSLTWSTEGLINEYGNPCQSVVDGKIVDAAPLEGLEEIEIDGTLYEAFNTSG
                     GLGSLAESHGKQCEQINYKTMRYPGHCAQMRLLMNDLKLNSDRATLKRVLEHAVPQTL
                     QDVVIVYAAVAGKQDGELREENYVNKIYPQMIAGRLWSAIQVTTAAGITAVVDLVMAS
                     PRKYQGFVAQEQFALPEILDNRFGQYYAPGGTKDISSVVVVSGQAGHQRKAAAKAAKA
                     AAGKSARPRVKARLKK"
     gene            3620..5191
                     /locus_tag="VEW08_02295"
     CDS             3620..5191
                     /locus_tag="VEW08_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYJ39589.1"
                     /translation="MSAAKAGTTVSVDVPGILKRLGVDSINAGAWMGNLGWSKDTAGS
                     AITSTNPASGETLAQVRSATVTDYENVMRCAVEAAAAWRDVPAPRRGEAIRLLGDALR
                     AAKDDLGALVTLENGKIKAEGLGEVQEMIDIADFAVGQSRMLYGTTMHSERPQHRMYE
                     QWHPLGVVGIISAFNFPVAVWAWNAFLAAICGNASVWKPSPKTPLTAIAVQHICNRVM
                     REHKLPAIFQLFIDGGTELATRFVDDRRVALVSFTGSTAVGRNVAERVARRLGKSLLE
                     LGGNNAIIVDETADLKLAVPAIVFGAVGTAGQRCTTTRRVLVHRSRIEELSKRLAHAY
                     TQVRIGNPIDPATLMGPLIDKGAVERYSQAIAQAKAAGGKVITGERILEGKGNFVAPT
                     IVLAENDWPIVQHETFAPVMYLIPFDSLDEALEMQNASAHGLSSAIFTDRLQHAEAFL
                     SAAGSDCGIANVNIGTSGAEIGGAFGGEKDTGGGRESGSDSWKAYMRRQTNTINWSRE
                     LPLAQGITFEIKEGP"
     gene            complement(5233..6600)
                     /locus_tag="VEW08_02300"
     CDS             complement(5233..6600)
                     /locus_tag="VEW08_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ39590.1"
                     /translation="MSRPSLRRRLMLSVFVVLVVFFGLTVFLLDMLFRRAAERSLREV
                     IDAQMVALVAAADPDGPESVTPTALLDPRFETPGSGLYAEIRSGSGETIWRSQSTTGT
                     TVQFGPAVEGGARTFFYTEIADTEIRLAVSSRGIVWPDLHGKPARFTFSVASNLKAYD
                     EQVSGFRQQLIGWFAGLALAFVATLALLLRWLGEPMRRLEREIREVESGTREKLGDEW
                     PIELAPVTGNLNALLAGERTRIRRYRDTLGNLAHSLKTPLAVMRQALGAGGGSKDALN
                     AEIDRMSGIIEHQMKRAAASGGVLLGQAPVDVATVVAELRGALLRVYGDKDMSIEAAI
                     APGAKFIGDRADLTELLGNLLDNACKWCRSRVRIEVSIEQGGDSRRELRLTIDDDGAG
                     IAEQDRARVLQRGGRADEATPGHGIGLSMVHDTVELYGGTMKIDNSSLGGARFDLALP
                     GRRAD"
     gene            complement(6623..7339)
                     /locus_tag="VEW08_02305"
     CDS             complement(6623..7339)
                     /locus_tag="VEW08_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ39591.1"
                     /translation="MRALVVEDEASLREGLRDQLVKQGFTVDVAADGEEGLFAGKEYP
                     LDVAIIDLGLPKLSGLDLIKALRTAGKKFPILILTARDRWQDKVEGLQAGADDYVAKP
                     YHFEEVLARMQALLRRAGGWASPILECGPIRLDTRAQAVTLNGSAIELTTFEYRILEH
                     MMLRAGDVISKTELTERLYDQDYERDSNVIEVFIGRLRRKLDPDEALQPIETLRGRGY
                     RFALVRTDMARSDMARPPGA"
     gene            complement(7344..7661)
                     /locus_tag="VEW08_02310"
     CDS             complement(7344..7661)
                     /locus_tag="VEW08_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HYJ39592.1"
                     /translation="MKTIRKGLLPIALLAVSFAPFTQAYGMRGQVVGGSAENRAGVIA
                     EQRDGISLDEAVRRAEAQYRAKVVRTDVQDEDGRKVYVLKLLSEDGRVVTVRVDAATG
                     RTR"
     gene            complement(7772..8413)
                     /locus_tag="VEW08_02315"
     CDS             complement(7772..8413)
                     /locus_tag="VEW08_02315"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HYJ39593.1"
                     /translation="MSISSRKIVVTLVAAAVSAASAGAFAQDPYGQDPYDQGRYEQDR
                     YGQNGHGGRYDDGAYDYARVVDVQPLVTRVRVSTPQRECWDETRVDNRGYGNSPLPRS
                     VAGGAVLGGIVGAVLGHQIGHGQGRDAATAVGAVVGAAVGSQQAQRRAAYASAPPQSY
                     TVQRCETRYRDEWQERTDGYRVTYVYNGRRQVTELPYRPGDRIRVRVDVSPAE"
     gene            complement(8479..9318)
                     /gene="mazG"
                     /locus_tag="VEW08_02320"
     CDS             complement(8479..9318)
                     /gene="mazG"
                     /locus_tag="VEW08_02320"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HYJ39594.1"
                     /translation="MSDDADAKAQRALVRLLGIMRQLRDPARGCPWDREQDFASIAPY
                     TIEEAYEVAEAIARGEPRALEGELGDLLFQVVFHAQMGAERRWFDFASVADAIADKLT
                     ERHPHVFADAHIASVADQNRAWEEHKARERAARGEATGELSDVPLALPALARAAKLGK
                     RAGRVGFDWPDAQGVRAKIEEELGEIEAVADGQAAHRAEELGDLLFAVANWARHLEVD
                     PEESLRLANAKFERRFRAMEKLAAKRALPLRTLSPAEWDGLWNEAKAAEKAPEKGEKT
                     GVQ"
     gene            complement(9315..9947)
                     /locus_tag="VEW08_02325"
     CDS             complement(9315..9947)
                     /locus_tag="VEW08_02325"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HYJ39595.1"
                     /translation="MSDLRSRILRRFEGSQPQHEPADWRLLGVDAERSRRLQRHFPVD
                     PVAAAVLVALVDRPEGLTVLLTERASQLAKHAAQISFPGGRLEPSDPDVASAALREAQ
                     EEIGLDPARVKVIGYLPDHLVISGFRVTPVLSLVTPPFSLTPNPEEVAEVFEVPVSHI
                     FDRDNHQARLRRVGDEDLLLYDIPWERHSIWGATAGMLLTFIRMVQEEES"
     gene            complement(9944..11353)
                     /locus_tag="VEW08_02330"
     CDS             complement(9944..11353)
                     /locus_tag="VEW08_02330"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HYJ39596.1"
                     /translation="MISQGDKVPGRKEHDSFGEIEVPAEALWGAQTERARFSTMIFGG
                     QLPRDFIHAVAQIKAAAALANARLGLLPSDIAGAIQGAVKDILYFRHDDQFPLDVFQT
                     GSGTSTNMNVNEVIAHLASKALGKPVHPNDHVNMCQSSNDVIPSAIHLSALSTSWLLH
                     AALGSLRSVLLEKEQEWANVLKTGRTHLMDAMPMTLGQEVSGWRAQIDNAIARLKSSE
                     PRLLALAQGGTAIGTGINAHEKFAATFIEELRAATGLDLTVAPNYFAAMGSQDAAVEY
                     SGQLRTLAVALMKISNDLRWMNSGPLAGLSEIRLPALQPGSSIMPGKVNPIVPEAVAM
                     AAVQVMGYDAAIAIAGQSGNFQLNVMLPLIANNLLRSISLLASACSSLASLALPKAQA
                     NVESLNEALSRNPILVTALNPVIGYEKAAAIAKRAYAEGRPVFDVALEMSGLKEDELR
                     RLLDPAALTRGGLHGGGQR"
     gene            11393..12757
                     /gene="purB"
                     /locus_tag="VEW08_02335"
     CDS             11393..12757
                     /gene="purB"
                     /locus_tag="VEW08_02335"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF006764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HYJ39597.1"
                     /translation="MDSAALLALSPVDGRYRRGAEPLRAVLSESGLIRERVRIEASWL
                     LALAESVPQLLPQPLSESTKGVAAALALEPGEDAPAAVKTIESRINHDVKAVEYFVRD
                     KLTAGGATPAQLELVHFGCTSEDINNLAYARMLRAARAVLSHELEKVIEKLRGFAHEH
                     AALAMLSRTHGQTASPTTLGKEFANVVARLRRARARLDKVDILAKWNGAVGNYNAHVA
                     ALPKVDWPAVSRKFIESQGITWNEYSTQIEPHDWIGEYCDALAGINTIVIDFARDTWG
                     YISLGYLRQRAVAGEVGSSTMPHKVNPIDFENAEGNLGIANALLHHFAAKLPISRWQR
                     DLTDSTVLRNVGVALAHSLIAWNALQRGLGKIAADPKTIAQDIDRAHEVLGEALQTAL
                     RAAGVPNGYELLKDFTRGTQVDAAGLAAFIDKLPLPATERTRLKALAPQQYLGLAAEM
                     ARSI"
     gene            12984..13538
                     /locus_tag="VEW08_02340"
     CDS             12984..13538
                     /locus_tag="VEW08_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39598.1"
                     /translation="MDFNRFSQRVSAPGAALPEVSGNVEQNQIRVISSLEEMRAAVEA
                     VAASAQRLMSIYTPDLEPDLYDQNGFLEIVKRFVLARRFAKARVLLSDSGRLLRDNNR
                     FIAMGRRLTSCIDIRPAVSSAKQRACGYLIADDRAIVYRVHSDRWDGVADLNNPAVAR
                     QYLDEFDEIWHASAPDELARQHLR"
     gene            13545..14030
                     /locus_tag="VEW08_02345"
     CDS             13545..14030
                     /locus_tag="VEW08_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYJ39599.1"
                     /translation="MRWNPEVTVAAIVEIDGRFLMVEERMGDRLVLNQPAGHLEDRET
                     LLEAAVRETREETAWRFHPEALVGVYLWRSPVTERSFLRVAFCGTVDDHQPKLPLDDG
                     IVRTLWMSYEQLLAQPARLRSPLVARCLDDFRRGARQPLESIASLGLDDAMHVDAVVS
                     L"
     gene            14082..15188
                     /gene="mnmA"
                     /locus_tag="VEW08_02350"
     CDS             14082..15188
                     /gene="mnmA"
                     /locus_tag="VEW08_02350"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HYJ39600.1"
                     /translation="MPPSSEKIVVGMSGGVDSAVSALLLKRAGFEVQGLYMSNWEDDD
                     SYCTGAQDFQDARAVAVELGIPLHRVSFAEQYRARVFEYFLTEYRAGRTPNPDVLCNR
                     EIKFGICLEYAARLGAQRFATGHYARLDHSGGQPVLLKARDPDKDQSYFLHAVEREHF
                     ARVMFPLGELTKSEVRALAREAGLPVFDKPDSTGICFIGERPFREFLARYIPTTPGDI
                     VTERGEIVGRHRGLAFYTLGQRGGLEIGGRAGQREDAWYVAAKNLARNELLAVQGHDH
                     PLLVSRALATGPCNWLVQPGTPEFDSQIKIRYRQADQTCRATVDETGALQIRFTEPQR
                     AVTPGQFAVLYDGERCLGGGVIERTWPQQNGLVS"
     gene            15231..>17093
                     /gene="acnA"
                     /locus_tag="VEW08_02355"
     CDS             15231..>17093
                     /gene="acnA"
                     /locus_tag="VEW08_02355"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HYJ39601.1"
                     /translation="MTGSSNTRSTLQVGSRSYDFWSFAKLPADKVARLPFSLKILLEN
                     LLRYEDGVNVTAADIQAVLDWDAKATPSYEIDFTPARVIMQDFTGVPCVVDLAAMREA
                     VKKLGGDPQRINPLNPAEMVIDHSVQVDDYGQATSLATNNRIEFERNGERYAFLRWGQ
                     TAFDNFSVVPPNTGIVHQVNLEFLGRVVFHKTAGDKSLAYPDTVVGTDSHTTMINGLG
                     VLGWGVGGIEAEAAMLGQPVTMLIPQVIGFKMNGAMKPGCTATDLVLTVTEMLRKKGV
                     VDKFVEFFGDGLANLPLADRATIANMAPEYGSTCGIFPIDEETLRYLELSGRPAEQIA
                     LVEAYAKAQGMWRINGARPADYTDVLELDLATVEPSLAGPKRPQDRVPLSKAKSVYQA
                     ALKVMAEERTKKNPTATGHAEIQLGGNQVDIRDGAVTIAAITSCTNTSNPYVLMAAGL
                     LARNAAKLGLKSKPWVKTSLAPGSRVVTDYLTKAGLLAPLESIGFNVVGYGCTTCIGN
                     SGPLKPEISAGIKAGDIVATSVLSGNRNFEGRVHPEIKMNFLASPPLVVAYALAGTMD
                     VDLQTEVLGHDSAGKPVLLANIWPSDKEVADAVHATITSAMFQKSYANVFAGDAN"
BASE COUNT         3121 a         5592 c         5313 g         3067 t
ORIGIN      
        1 gatgccgatg ccgcccacct gcgccgccag cgcgtcggag atgtagtcgc cattgaggtt
       61 gagcgtggcg atcacgtcgt agtcttccgg ccgcatcagc acctgctgca ggaagttgtc
      121 ggcgatcacg tccttgacca cgatctcctt gccgttgatc gggttcttga aggagcacca
      181 cggtcccgcg ccgatgggct gcgcgccgta ctcctccttc gccagtagat agccccattg
      241 catgaaggca ccttcggtgt acttcatgat gttgcccttg tgcaccagcg acaccgacac
      301 gcggtcgttt tcgatggcgt actggatggc cttgcgcacc aggcgctggc tgccctcctt
      361 cgacaccggc ttgatgccga tgccggaact cgccgggaaa cggatacccg tggccttgag
      421 ctccgattgg aggaaatgga tgagcttgtg gacttcctgc gaacccgtgg gccactcgat
      481 gcccgcatag atgtcttcgg tgttctcgcg gaacaccacc atgtcggtga gcgacgcgtc
      541 cgccatgggc gtggagacgc cggggaaata gcgtatgggg cgcacgcagg cgtacaggtc
      601 gaggttctgg cgcatggcca cgttcaacga acgcatgccg ccgcccaccg gtgtgccgag
      661 cgggcctttg atggagacca cgaagtcacg aagcgcggtc agcgattcgt cggggaagcc
      721 ggtgccgtag accttggcgg ccttctcgcc ggcgaaaacc tccacccagg aaatccggcg
      781 cctgctcccg taagcctttt cgaccgcggc attgaccact ttcaacatca ccggcgtgat
      841 gtcgatgccg atgccatcgc cttcgatgaa ggcgatgatc gggttttccg gcacgttcag
      901 cgacgagtcc ggattgatgg tgatgcgctg accgtctttg ggaaccgcta ttttttcgta
      961 ctgcatggtc cggaaacgag caattaacgt gccggaccgt gacggccgat gagcatcgcc
     1021 atctcgagcg cctgttcgta gttcagccgg ggatcgaccg tggacttgta agcacgcgcg
     1081 aggtccgcat cggtgaggcc gcgagcgccg ccggtgcatt cggtgacgtc ttcacccgtg
     1141 agctcgatgt gcacgccgcc gaggaacgat ccattggctt cgtgaacctg gaacgacaat
     1201 tcgagttcct tgaggatgtt ctcgaagcgg cgcgtcttca ggccaccgga actggtctcc
     1261 gtgtttccat gcatggggtc acaaacccac aacacctgct ggcccgtttc gcgcacggcc
     1321 ttgatcatct gcggcaggcc tttttccaca tccttcgcgc cgaagcggtg aatgatcgtg
     1381 aggcgtcccg gtgtgttctg cggattgagc gtggtcacga ggccctgcag ccattccgtg
     1441 gtcatcgccg gcccgatctt cacgcccatg gggttggaga tgccgcggaa gaattccacg
     1501 tgtgcgccat cgagggcggc cgtgcgcatg ccgatccacg gcatgtgcgt tccgaggttg
     1561 taccagcgct tctggcgttc gatgtaacgc gtctgcgcct gctcgtaagg catgagcagg
     1621 ccttcatgcg ccgcgaagaa gtcgaccgcg gtgacttcgt gcggcgcgcg gccgccgctc
     1681 acgcgttcga agaagtccag ggagtcaccg atctcatcga cgatcttctg gtactgcgcc
     1741 accagcggcg aatgcttcat gaagccgaga tcccaatatc tcgggtgatg cagatcggcg
     1801 aacccgccat cacacagcgc acgcacgaaa ttcagcgtga gcgccgcgcg ttcatagccg
     1861 cgcagcagca aggtgggatc cggttcgcgc gccacggcgg taaactcgga acgattcacg
     1921 agatcgccgc ggaatgccgg cagcgtgatg ccgttgcggg tttcggtatc ggcggaacgg
     1981 ggcttggcgt attgcccggc catgcggccg acgcggatga tgggtttctt gagcccgtgc
     2041 agcagcacga ggctcatctg cagcaggatc ttgagcttct tggcgatctt gtcggactcg
     2101 cactcttcga acgtctcggc acaatcgccg cccatgagca ggaatttgcg gccgatctgc
     2161 gcctcggcga gccgttcgcg taactgctcg atttcccagg acacaaccag cggcggcagc
     2221 cgggacaggg atgcgaccgc ggtttcgaga gcggcggcgt cgttatatcg cggctgttgg
     2281 gctgcggtgc gggaggtcca gcttgctgga tgccaggggg tggtgtcggg ggtacgcatg
     2341 gtgggaagta ttctaattca gcaggttacg gcacactacc gtcaatcgag gtattcatgc
     2401 acaaagttct gatcctgggt gccggcaaga tcggcgccct catctctggt ttgctggcgg
     2461 agtcgggtga ttatgacgtc actctcggtg acgtcgatgc cgtggcggct gaagctgtcg
     2521 tgaaggctca cgggtcgcac aacctgcggt ccgtggcgct ggacgcgagc gaccccaagg
     2581 cgctcgaaaa tcatttctcc gcccacaaac cacaagcggt catctccagc ctgccctact
     2641 actgcaatcc ggaggtggcc aaagccgcgc gcaaaggcaa ctcccactat ttcgatctca
     2701 ccgaagacgt ggccgtcacc cagggcgtgc gacgcctcgc gcgtggtgca acgaaggcat
     2761 tcgtgcccca atgtggactt gcaccgggct tcatcagcat cgccgcgaac gaactcacca
     2821 agcatttcga cgagcttcgg tccatcaagc tccgcgtcgg cgccctgcca cagcacccta
     2881 acaacgtgct caagtattcc ctgacgtggt ccaccgaggg cctcatcaac gaatacggca
     2941 acccgtgtca gtcggtggtc gacggcaaga tcgtcgatgc cgcgccgctc gagggcctcg
     3001 aggaaatcga aatcgacggc acactgtacg aagcgttcaa tacttcgggc ggattgggct
     3061 cgttggccga gtcgcacggc aagcagtgcg agcagatcaa ttacaagacg atgcgttacc
     3121 ccgggcattg cgcgcagatg cgcctgctca tgaacgatct caagctcaat tcggatcgcg
     3181 ccaccttgaa gcgcgtgctc gaacacgctg tgccgcagac attgcaggac gtcgtcatcg
     3241 tctacgcggc cgtcgcgggc aagcaggacg gcgaactgcg cgaggaaaat tacgtcaaca
     3301 agatctatcc gcagatgatc gcgggccggc tctggtcggc gatccaggtc accaccgccg
     3361 cgggaatcac cgcggtggtc gatctcgtga tggcgagtcc acgcaagtac cagggattcg
     3421 tcgcgcagga acaattcgcg ctgccggaaa ttctcgacaa ccgttttggc cagtactacg
     3481 cgccgggcgg caccaaggat atttcctcgg tggtcgtggt cagcggccag gccgggcatc
     3541 agcgcaaggc cgcggcgaaa gccgccaagg ccgccgcggg caagtccgcc cgtcccaggg
     3601 tcaaggcgag gctcaagaaa tgagcgccgc caaggcaggc accactgtca gcgtcgatgt
     3661 cccgggcatt ctcaagcggc tcggagtcga ttcgatcaac gccggtgcct ggatgggcaa
     3721 cctgggctgg tccaaagaca ccgcgggcag cgccatcacg tccaccaatc cggcgagcgg
     3781 tgaaactctt gcgcaggtgc gcagcgctac cgtcacggac tacgagaacg tcatgcgctg
     3841 cgccgtcgag gccgccgccg cgtggcgtga tgtgccggcc ccgcgccgcg gtgaggcgat
     3901 ccgcttgtta ggcgatgcgc tgcgcgccgc caaggacgac ctgggcgcgc tggtgacgct
     3961 cgagaacggc aagatcaagg ccgaggggtt gggcgaagtc caggagatga tcgatattgc
     4021 agatttcgcc gtcggccagt cgcggatgct ctacggcacg acgatgcatt cggaacggcc
     4081 gcagcaccgc atgtacgagc agtggcatcc gctcggcgtg gtcgggatca tctccgcgtt
     4141 caattttccg gtggccgtct gggcgtggaa cgcatttctc gccgccatct gcggcaacgc
     4201 ttcggtatgg aagccttcgc ccaagacgcc gctgacagca atagccgttc agcacatctg
     4261 caaccgcgtg atgcgcgagc acaaactacc cgccatcttc cagttgttca tcgatggcgg
     4321 cacggagctc gccacgcgat tcgtggatga ccggcgtgtt gcgctggtgt cgttcacggg
     4381 atccacggcc gtaggccgga atgtcgcgga acgggtcgcg agacgcctcg gcaagtcgct
     4441 gctcgagctc ggcggtaaca acgccatcat cgtcgacgag acggcggatc tgaaactcgc
     4501 agtgccggct atcgtgttcg gcgccgtggg aacggctggc cagcgttgca ccaccactcg
     4561 acgcgtgctc gtgcatcgtt cgcgcatcga ggagctgtcg aagcggctgg cgcatgccta
     4621 cacgcaggtg cgcatcggca acccgatcga tccggcgacg ctcatgggcc cgctgatcga
     4681 caagggcgcc gtcgaacgct actcacaggc catcgcgcag gccaaggccg ccggcggcaa
     4741 ggtgatcacc ggcgagagga ttctcgaggg caagggcaac ttcgtcgcgc ccacgattgt
     4801 cctcgcggaa aacgattggc ccatcgtgca gcacgaaacc ttcgcgccgg tcatgtacct
     4861 gattcccttc gactcgctcg acgaagccct cgagatgcag aacgcatcgg cgcatggcct
     4921 ctcctcggcg atcttcacgg atcggctgca acacgccgaa gcgttcctct ccgcggccgg
     4981 aagcgactgc ggcattgcga acgtgaacat cggcacctcg ggcgccgaga tcggtggtgc
     5041 gttcggcggc gagaaggaca caggcggagg acgcgagtcc ggctcggatt cgtggaaggc
     5101 gtacatgcgc cgccagacca acaccatcaa ctggagccgc gagctgccgc ttgcgcaggg
     5161 catcacgttc gagatcaaag aaggcccgtg aagttcgtgc cctcgcggca ggagaactga
     5221 tgcgcggatc ggctagtcgg ctcgtcgtcc cggcagcgca aggtcgaagc gcgcgcctcc
     5281 caggctggag ttgtcgattt tcatggtgcc gccgtacaac tccacggtgt cgtggaccat
     5341 cgacagcccg atgccgtgac cgggcgtggc ttcgtcagcg cgaccgccgc gctgaaggac
     5401 gcgcgctcga tcctgctccg cgatgccggc gccgtcgtca tcgatcgtca gccgtaactc
     5461 gcgacgcgaa tcgccgccct gctctatcga cacttcgata cgcacacgcg aacggcacca
     5521 cttgcaggcg ttgtcgagca gattgccgag cagctcggtg agatcggcgc gatcaccgat
     5581 gaacttcgcg cccggcgcga tcgccgcctc gatcgacatg tccttgtcgc cgtaaaccct
     5641 tagcaatgcg ccgcgcagct cggcgacaac cgttgccaca tccaccggcg cctggcctaa
     5701 caacacgccg ccactggctg cggcgcgttt catctggtgc tcgatgatgc cgctcatgcg
     5761 gtcgatctcg gcattgagcg catccttgct gccaccgccc gcaccaagcg cctgccgcat
     5821 gaccgcaagc ggtgtcttga gactgtgggc gaggttgccc agcgtgtcgc gataacgccg
     5881 gatgcgcgta cgctcaccgg ccagcagcgc attgaggttg ccggtgaccg gagcgagctc
     5941 gatcggccat tcatcgccga gtttctcgcg agtacccgac tcgacctcgc gaatctcacg
     6001 ctcgagtcga cgcatcggtt caccgagcca gcgcagcagc aacgccaatg tcgcgacgaa
     6061 ggccagcgcg agcccggcaa accagccgat cagttgctgg cgaaagcccg acacctgttc
     6121 gtcgtacgcc ttgagattgg atgcgacgct gaaggtgaag cgtgccggct tgccatgcag
     6181 gtcgggccag acgatgccgc ggctcgagac cgcgagccgg atctcggtgt cggcgatttc
     6241 cgtatagaaa aaagtgcgcg cgccgccttc gaccgcgggg ccgaactgta ctgtcgtacc
     6301 ggtggtcgac tgggaacgcc agatggtctc accgctgccc gaacgaatct cggcatagag
     6361 gccggaaccc ggtgtctcga atcgcggatc gagcaatgcg gtgggcgtga cggattcagg
     6421 gccgtcagga tcggcggcgg caacgagcgc gaccatctgc gcgtcgatca cttcgcgcag
     6481 gctgcgttcg gcggcgcggc gaaagagcat gtcgagcagg aacacggtaa gcccgaagaa
     6541 caccaccagc acgacgaaca ccgacagcat gagtcggcgt ctgagcgatg gacgggacac
     6601 cggtggctac ttccggcgcg gcttacgcgc ccggggggcg cgccatgtca ctgcgagcca
     6661 tgtctgtccg aacaagggcg aagcggtagc cgcgtccgcg cagggtctcg atgggttgca
     6721 gcgcttcgtc gggatcgagc ttgcggcgca ggcgcccgat gaacacttcg attacgttgc
     6781 tgtcgcgctc gtaatcctgg tcgtacaggc gctcggtaag ttcggtcttg gagatcacgt
     6841 cgccggcacg cagcatcatg tgttcgagga tgcggtattc gaatgtcgtg agttcaatgg
     6901 cgctgccgtt caatgtgacg gcctgggcgc gcgtgtccaa ccggatgggg ccgcattcga
     6961 gaatgggact ggcccacccg cctgcccgcc gcaacagcgc ctgcatgcgt gccagcactt
     7021 cttcgaagtg atagggcttc gcgacatagt cgtcggcgcc ggcctgcagc ccttcgacct
     7081 tgtcctgcca gcgatcgcgc gcggtcagga tgagaatcgg gaatttcttg cccgcggtgc
     7141 gcagggcctt gatgagatcg agcccggaga gtttgggaag cccaaggtcg atgatggcga
     7201 catcgagcgg gtattccttg ccggcgaaca gcccctcttc gccgtcggcg gccacgtcga
     7261 ccgtgaagcc ctgcttcacc agctgatcgc gcaggccttc gcgcagggaa gcttcgtcct
     7321 cgacaaccag tgcacgcatg cggtcagcgc gtccttccgg ttgcggcgtc gacacgcacg
     7381 gtgacgacgc gtccgtcctc ggagagcagc ttcagcacat acaccttgcg cccgtcttcg
     7441 tcctgcacat cggtgcgtac gaccttggcc cggtattgcg cttcggcacg ccgcaccgcc
     7501 tcatcgagcg agattccgtc gcgctgctcg gcgatcacgc cagctcgatt ttcagcactg
     7561 ccacccacca cctgcccccg catcccgtag gcctgcgtga atggcgcgaa actcaccgcc
     7621 agcagggcga tgggcaacag gcctttgcgg atcgttttca tgacgggtgc gagtttactg
     7681 gtttgcgccg ccaaataggc agtgaaggcg atgcgggtaa ccctcgaatc agaggcgcct
     7741 acccacaccg ccatggcaaa caactcgttc actactccgc cggactcacg tcgacccgga
     7801 cgcggatgcg atcgccgggg cggtacggca gctcggtcac ctggcggcgg ccgttgtaca
     7861 cataggtcac gcgataaccg tcggtacgct cctgccattc atcgcgatac cgcgtctcgc
     7921 agcgctgcac cgtatatgac tgcgggggcg cggaggcgta ggccgcgcgg cgctgcgcct
     7981 gctgcgaacc gaccgcggcg ccgacgaccg caccgaccgc cgtcgcggca tcgcgcccct
     8041 ggccatggcc aatctggtgg ccgagaaccg cgcccacgat gccaccgagc actgcaccgc
     8101 cggccacgga acgcggcagc gggctgtttc cgtatccacg attatcgacg cgggtttcat
     8161 cccagcattc acgctgcgga gtactgacgc gcacacgagt gaccagcggc tgcacgtcga
     8221 ccacgcgggc gtagtcgtag gcgccatcgt cataacgacc gccgtggcca ttctggccat
     8281 aacggtcctg ttcatagcgg ccctgatcgt aaggatcctg accgtacgga tcctgggcaa
     8341 acgcgccggc gctcgccgcc gacaccgccg cggccaccag tgtcaccacg atctttctgc
     8401 tgctgatcga catatagcct ccgtcggtcg aggggtatcc cgaccgtggg gccaatgtaa
     8461 ggagcagctg atgaatcctt actgaacgcc ggttttctcg cctttttccg gcgctttttc
     8521 cgcggctttt gcctcgttcc agaggccatc ccactcggcg ggcgacaggg tcctgagagg
     8581 caacgcacgt ttcgccgcaa gcttttccat ggcgcgaaag cggcgctcga acttggcgtt
     8641 ggcgaggcgc agcgactcct cggggtccac ttcgagatgc cgcgcccagt tcgcaaccgc
     8701 gaacaacagg tcgcccagct cctcggcgcg atgcgcggcc tggccatcgg ccaccgcttc
     8761 gatttcgccg agctcctctt ctatcttcgc gcgaacgccc tgggcgtcgg gccagtcgaa
     8821 gccgacgcgc ccggcacgtt tacccagctt ggcggcgcgc gccagcgccg gcaatgccag
     8881 cggcacgtcg gaaagttcac cggtcgcctc gcctcttgcc gcgcgctcac gcgccttgtg
     8941 ctcttcccac gcgcgattct gatcagcgac agaggcaatg tgcgcatccg cgaacacgtg
     9001 cggatgccgt tcggtgagtt tgtcggcaat ggcgtccgcc acgctcgcga aatcgaacca
     9061 tcgccgttcc gcgcccatct gcgcgtgaaa cacgacctgg aacaggagat caccgagctc
     9121 gccttcgagc gcccgcggtt cgccgcgcgc gatggcttcg gctacttcgt aggcctcttc
     9181 tatcgtgtac ggcgcgatgc tcgcaaaatc ctgctcgcgg tcccagggac agccgcgcgc
     9241 gggatcgcgc aattgacgca tgatgcccag caagcgcacc agcgcgcgct gcgctttcgc
     9301 gtccgcatca tcgctcacga ctcctcctcc tgaaccatgc gtatgaaagt cagcagcatt
     9361 ccggcggttg caccccagat gctgtgccgc tcccacggta tgtcatacaa cagaagatcc
     9421 tcgtcgccca cgcgccgcag gcgcgcctgg tgattgtccc ggtcgaaaat gtggctcacg
     9481 gggacctcga acacctcggc gacttcctcg ggattcggcg tgagcgaaaa cggtggagtc
     9541 accagagaca ggaccggcgt cacgcgaaag ccgctgatga ccaggtgatc gggtagatag
     9601 ccgatgactt tcactcgcgc ggggtcaagg ccgatctcct cctgcgcctc gcgcagcgcg
     9661 gcgctcgcga cgtcgggatc ggatggctcg agacggccgc ccgggaatga gatctgcgcc
     9721 gcgtgcttgg cgagttgcga ggcacgttcc gtgagcagca ccgtgagtcc ttcgggccgg
     9781 tccacgagcg cgaccagaac cgcggccgcc accggatcga cgggaaaatg ccgttgcaac
     9841 cggcggctgc gttccgcatc gacgccgagc aggcgccagt cggccggttc gtgctggggc
     9901 tgcgaccctt cgaagcgccg caggatccgg ctgcgcaggt cactcaacgc tgtcctccgc
     9961 cgtgcagtcc cccgcgcgtc agcgcggccg gatccaggag gcgccgcaat tcgtcttcct
    10021 tcagtcccga catctcgagg gccacgtcga ataccggccg cccttcggca taggcgcgct
    10081 tggcgatggc cgccgccttt tcatagccaa tgaccggatt gagcgcagtc accagtatcg
    10141 gattgcgcga cagcgcctcg ttgagacttt cgacattcgc ctgcgccttc ggcagcgcca
    10201 ggctcgcgag cgacgaacat gcgctggcaa gcagagatat ggaccgcaag agattgttgg
    10261 caatcagcgg caacatcaca ttcagctgga aattgcccga ctgtcccgcg atcgcgatcg
    10321 ccgcgtcgta tcccatgacc tgcaccgcag ccatggcgac ggcttcgggc acgatgggat
    10381 tcaccttgcc cggcatgatg ctgctgcccg gctggagcgc aggcaaccgg atctcggaaa
    10441 gacccgcaag aggcccgctg ttcatccagc gcaagtcatt gctgatcttc atgagcgcga
    10501 cggcgagagt tcgcaactgg cccgaatact cgaccgctgc atcctgggag cccatggccg
    10561 caaagtagtt tggcgcaaca gtgaggtcga ggccggttgc ggcgcgaagc tcttcgatga
    10621 acgtcgccgc aaatttttcg tgcgcgttga tgccggttcc aatcgccgtt ccgccctgtg
    10681 ccagcgccag caatcggggt tcgctcgact tgaggcgtgc gattgcgttg tcaatctgcg
    10741 cgcgccaccc ggacacttcc tgtccgagcg tcatcggcat tgcatccatg agatgggtgc
    10801 ggccggtctt gaggacgttc gcccattcct gctctttctc cagcaggacg ctacgaagac
    10861 ttccgagagc cgcgtgaagc aaccagctcg tcgagagcgc gctcaggtgg attgcactcg
    10921 ggatcacgtc gttgctgctc tggcacatgt tgacgtggtc gttcggatgc accggcttgc
    10981 cgagcgcctt gctcgcgagg tgcgcgatca cctcgttcac gttcatgttg gtgctggtgc
    11041 cgcttcccgt ctggaaaacg tcgaggggaa actgatcgtc atggcgaaaa tacagaatgt
    11101 ccttcacggc accctggatc gcaccggcga tatccgacgg cagcaagccg agccttgcgt
    11161 ttgcgagcgc ggccgcagcc ttgatctgtg cgacggcatg gatgaagtcc cgtggtaact
    11221 ggccgccgaa aatcatggtg ctgaagcggg cccgctcggt ttgagccccc cacagcgctt
    11281 cggcaggcac ttcgatctcg ccgaagctgt cgtgctcttt tcggccagga accttgtcgc
    11341 cttgggatat catgcgcggc cagtttagcc cccgattcga ggcatgtctt tcatggattc
    11401 cgcagcactc ttggcgctct ctcccgtcga cggccgttat cgcaggggcg cggagccgct
    11461 ccgcgcggtg ttgtcagaga gcggcctcat tcgcgagcgc gtgcgcatcg aagcgtcgtg
    11521 gttgctggcg ctggccgaat ccgtcccgca actcctgcct cagccgctca gtgaatcaac
    11581 gaaaggcgtg gccgcggccc tggcgctgga gcccggcgaa gacgcgccgg ccgcggtgaa
    11641 gaccatcgag agccgcatca accacgacgt gaaggcggtg gaatatttcg tgcgcgacaa
    11701 gctcaccgcg ggtggcgcca cgcccgccca gctcgagctc gtgcacttcg gctgcacttc
    11761 cgaagacatc aacaacctgg cttatgcgcg catgttgcgc gcggcccgcg ccgtgctgtc
    11821 gcacgaactc gaaaaggtca tcgagaagtt gcgtggcttc gcgcacgaac acgccgcgct
    11881 cgccatgctc tcgcgcacgc acggccagac agccagtccc accacgctcg gcaaggaatt
    11941 tgcgaacgtg gtcgcgcggc tgcgtcgcgc ccgcgcgcgg ctcgacaagg tcgacatcct
    12001 cgccaagtgg aacggcgcgg tcggcaacta caacgcgcac gtcgcggccc tgcccaaggt
    12061 cgactggccg gccgtaagcc ggaagttcat cgaatcgcag ggcatcacgt ggaacgagta
    12121 ctccacgcag atcgagccgc atgactggat cggcgagtac tgcgacgcgc ttgccggcat
    12181 caacaccatc gtcatcgatt tcgcgcgcga cacctgggga tacatctcgc tcggctacct
    12241 ccgccagcgc gcggtcgccg gtgaagtggg ctcctccacc atgcctcaca aggtgaatcc
    12301 catcgatttc gagaacgccg aaggcaatct cgggatcgcc aacgcgttgc tccatcactt
    12361 cgccgccaaa ctacccatct cgcgctggca gcgcgacctc acggactcca cggtattgcg
    12421 caatgtcggc gtggcgctgg cgcacagcct gatcgcctgg aatgcgctgc agcgcggact
    12481 cggcaagatc gccgccgatc cgaaaaccat tgcgcaggac atcgatcgcg cgcacgaagt
    12541 gctcggcgaa gcgctgcaaa ctgcgttgcg cgccgcgggc gtacccaacg ggtacgagct
    12601 gctcaaggac ttcacgcgtg gcacgcaggt ggatgcagcc gggctcgccg ccttcatcga
    12661 caaactaccg ctgccggcga ccgaacgtac gcggttgaaa gcccttgcgc cgcagcagta
    12721 cctgggtctc gccgcggaaa tggcgcgttc catctagaac tcgtctcgcc gaaaaaatgc
    12781 tgctgttgcg gcatatcggt atctgctgat gcctcttcgg atatgtgacg cataaagtgc
    12841 gaaccagcac acgttttccg gccggttacg cgtaaaaccc gatgaaagcg tgacgcattt
    12901 ggcactgaaa tagccatgcg ccacctaggc ttgagcgggc acaggtcaca aagcgacctc
    12961 tagtttcaat gactaccggg gaattggatt tcaacagatt ctcgcagcgc gtatcggcgc
    13021 cgggagccgc tttgcccgaa gtttccggca acgtcgagca gaaccagatc cgggtgatca
    13081 gctcgctcga agagatgcgt gctgccgtcg aagccgtggc tgccagcgca cagcggctca
    13141 tgtctatcta cacacccgac ctcgagcccg acctttacga ccagaacggg ttcctcgaga
    13201 tcgtcaaacg cttcgtcctc gcacgccgct tcgccaaggc tcgcgtgctg ttgtcggact
    13261 cgggcaggct gctccgcgac aacaaccgct tcatcgccat gggccgaagg ctcacgagct
    13321 gcatcgacat ccgccccgcg gtcagcagcg cgaaacaacg cgcctgcggc tacctgatcg
    13381 ctgacgaccg cgccatcgtc tatcgggtgc actcggaccg ctgggacggc gtcgccgacc
    13441 tcaacaatcc ggcggtcgcc cgccagtatc tcgatgaatt cgacgagatc tggcatgcca
    13501 gtgcacccga cgagctcgcc cgccagcacc tgcgttagtt agccatgcgc tggaatcccg
    13561 aagtcaccgt cgccgccatc gtcgagatcg acggccgttt tctcatggtc gaggaacgca
    13621 tgggggaccg cctggtactg aaccagccgg cgggccatct cgaagatcgt gaaaccctgc
    13681 tcgaggcggc ggtccgcgaa acccgcgaag agaccgcatg gcgattccac cccgaggcgc
    13741 tcgttggcgt gtacctgtgg cggagccccg tcaccgaacg cagcttcctg cgcgttgcat
    13801 tttgcggcac cgtcgacgat caccagccga aactcccgct cgacgacggc atcgttcgta
    13861 cgctgtggat gtcgtacgag cagctcctgg cgcaacccgc gcgactgcgc tcgccgctgg
    13921 tggcgcgctg cctcgatgat ttccggcggg gcgcgcggca gccgctggaa tcgatcgcca
    13981 gcctgggact cgacgacgcc atgcacgtgg acgcagtcgt cagcctctag agaaccagcg
    14041 cgcgaatcgc ttcgattgcc ttccggcgta caattcgcgc cgtgccaccc agttcagaaa
    14101 aaatcgtcgt cggcatgtcc ggcggtgtgg actccgccgt ttccgcactg ctgctgaagc
    14161 gcgccggctt cgaagtgcag ggcctctaca tgagcaactg ggaggacgat gactcctact
    14221 gcaccggcgc gcaggacttc caggatgcgc gcgccgtggc cgtcgagctc ggcattccgt
    14281 tgcaccgcgt gagtttcgcc gagcagtatc gcgcgcgggt gttcgaatac ttcctgaccg
    14341 agtaccgcgc gggccgtacg ccgaatcccg atgtcctctg caatcgcgag atcaagttcg
    14401 gcatctgcct cgagtacgcg gcacggctgg gcgcgcaacg cttcgccacc ggccactacg
    14461 cgaggctgga tcacagcggc ggccaaccgg tgctgctcaa ggcgcgcgat cccgacaagg
    14521 accagtccta cttcctgcac gccgtcgagc gcgaacattt cgcgcgcgtg atgtttccgc
    14581 tcggtgaact caccaagtcc gaagtgcgcg cgttggcgcg cgaggcaggg ttacccgtat
    14641 tcgacaaacc cgacagcacc ggcatctgtt tcattggcga gcggcccttc cgcgaatttc
    14701 tggcgcgata catccccacg actccgggcg atatcgtgac cgagcgtggc gagatcgtcg
    14761 gtcgccaccg gggactcgcg ttctacacgc tggggcaacg cggtggcctc gagatcggcg
    14821 gccgcgcggg ccagcgcgaa gacgcctggt acgtggcggc caagaatctt gcccgcaatg
    14881 aactgctcgc agttcagggg cacgatcacc cgctgctcgt aagtcgtgcg ctggccacgg
    14941 ggccctgcaa ctggctggtg cagccgggga cgcccgagtt cgattcgcaa atcaaaatac
    15001 gctatcgaca ggccgaccag acctgccgtg cgaccgtgga tgagaccggc gcgctccaga
    15061 tccgtttcac cgaaccgcaa cgtgcggtga cccccggaca attcgcggtg ctgtatgacg
    15121 gcgaacgctg cctcggcggg ggtgtcatcg agcgcacgtg gccgcaacag aatgggcttg
    15181 tgagttagaa tgcgcggctt gttttcgccg agactgatgg agaatcattc atgactggca
    15241 gttccaatac ccgttcgacc ctgcaagtgg gctcacgctc ctacgacttc tggagcttcg
    15301 cgaaacttcc cgctgacaag gtcgcccgac tgcccttctc gctcaagatc ctgctcgaaa
    15361 acctgctgcg ttacgaagac ggcgtcaacg tcaccgcagc cgacatccag gccgtgctcg
    15421 actgggatgc caaggccacc ccgtcgtacg aaatcgattt cacccccgcg cgagtgatca
    15481 tgcaggactt caccggcgtg ccctgcgtgg tggacctcgc cgccatgcgc gaagcagtga
    15541 agaagctggg tggcgacccg caacgcatca atccactgaa ccccgcggag atggtcatcg
    15601 accattcagt gcaggttgac gactacggcc aggctacttc gctcgcgacc aacaaccgca
    15661 tcgaattcga acgcaacggc gagcgttacg ctttcctgcg ctgggggcag accgctttcg
    15721 acaatttcag cgtcgtcccg ccgaataccg gcatcgttca ccaggtcaat ctcgaattcc
    15781 ttggccgcgt cgtgttccac aagaccgccg gtgacaagtc gctcgcctat ccagacaccg
    15841 tcgtcggtac cgattcgcac accaccatga tcaatggcct gggcgtgctc ggctggggcg
    15901 tgggtggcat cgaggcggaa gccgcgatgt tgggccagcc agtgaccatg ctcattccgc
    15961 aggtcatcgg cttcaagatg aacggtgcga tgaagcccgg ctgcaccgcc acggatctcg
    16021 tgctcacggt caccgagatg ttgcgcaaga aaggcgtggt cgacaaattc gtcgagttct
    16081 tcggcgacgg cctcgccaat ctgccgctcg cggatcgcgc cactattgcc aatatggccc
    16141 ccgagtacgg cagcacttgc ggcatcttcc cgatcgacga agaaacgctg cgttatctcg
    16201 agttgtcagg ccggccggcc gagcaaatcg cattggtcga ggcctatgcc aaagctcagg
    16261 gcatgtggcg catcaacggc gccaggcccg cggactacac cgacgtgctc gagctcgacc
    16321 tcgccaccgt ggagcccagc cttgccgggc cgaagcgccc gcaggatcgc gtgccgctgt
    16381 cgaaagccaa gagcgtctac caggctgcac tcaaggtcat ggccgaagag cgcacgaaga
    16441 aaaaccccac cgccacgggt catgcggaaa tccagctggg cggaaaccag gtggacatcc
    16501 gcgacggcgc ggtaaccatt gccgccatca cgagctgcac caacacttcc aatccgtacg
    16561 tgctcatggc cgcgggcctg ctcgcgcgca atgccgcgaa gctcggcctc aagtcgaaac
    16621 cctgggtcaa aaccagcctg gcccccggat cacgcgtggt cactgactac ctgaccaagg
    16681 ccgggttgtt agcgccgctc gagtcgatcg gcttcaatgt cgtcggctac ggctgcacca
    16741 cctgcattgg caactccggc cccttgaagc cggaaatttc cgcgggcatc aaggccggcg
    16801 acatcgtcgc cacctcggtg ctttcgggca atcgcaattt cgaaggccgc gtgcaccccg
    16861 agatcaagat gaacttcctg gcctcgccgc cgctcgtggt ggcgtatgcg ctcgcgggca
    16921 ccatggacgt ggaccttcag accgaggtgc tgggacacga ctccgcgggc aaacccgtat
    16981 tgctggccaa catatggccc tccgacaagg aggtcgcgga cgcggtacat gccacgatca
    17041 cctccgccat gttccagaag agttacgcca atgtcttcgc cggcgatgcc aac
//
LOCUS       DASQJS010000061         3833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_34069, whole genome shotgun sequence.
ACCESSION   DASQJS010000061 DASQJS010000000
VERSION     DASQJS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34069"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VEW08_02360"
     CDS             <1..609
                     /locus_tag="VEW08_02360"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ39602.1"
                     /translation="HRQLLLELNHVINVHSRLREVAADVVPPDDWTSWQDLLAVPALA
                     FEQGRSFLDDLRALGLNPMDSVSLTPLPQTIALAAYAHYQLVERATVGLVGYLWFFER
                     MPRLLYPLWSESCRRGGVPEKALRALAEGAVVHAVRDQAVVNCCRQIVRRPRDLGLAT
                     QSLHDTAELFGTMLDAALQRAERRHPLAVESVVVAGGIGQVA"
     gene            645..1571
                     /locus_tag="VEW08_02365"
     CDS             645..1571
                     /locus_tag="VEW08_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HYJ39603.1"
                     /translation="MPDTLYHPRVIYLIDASVYVFRAYYSMPPDMVDSDGNPAHATFG
                     FARFLGDLIERTQPRYIAVAFDESLTTSFRNQIYPAYKANRDPAPADLKLQFARCREF
                     CRHAGIPAFSHAEYEADDIVGTLMTRCRSEGLRATLVTRDKDYAQLIRTGDVYWDYSD
                     NVRYRYDEIEDRFGVQPERFADFLALMGDSVDNIKGVPGVGAKTAAALMKEFVSLEEI
                     YDNLDRVGDIAVRGAGKLAAKLIEHKEAAFFARRLTEIACNMPLETTRMDLIRRAPDV
                     SALTAFFDRHNFGPMLRRQAERLALLPLAAAA"
     gene            complement(1581..2441)
                     /locus_tag="VEW08_02370"
     CDS             complement(1581..2441)
                     /locus_tag="VEW08_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HYJ39604.1"
                     /translation="MRFRYPLVLLALVPATALAWGDGCKLRADRAGGIEAAGVEKVVI
                     RAGAGDMKVVGRGNAVRIEARGVACAAKQELLDATQISVRREGNVVYVETTLPQDDGK
                     WSWGDNEYAYIDIGIAVPSNIPVEATDSSGDATFEDLNALTLQDSSGDINLNRIAGLA
                     DVGDSSGDIEIETAGSVRVRDSSGDIDVDDVKGDVDVVLDSSGDIEIAKVSGNVRVEQ
                     DSSGGIRVEDVKGSVSVDSDSSGDIYAGSVSGDFTVREDSSGSIEHESIGGRVTVPNN
                     KRNDDDRDVE"
     gene            2558..>3833
                     /locus_tag="VEW08_02375"
     CDS             2558..>3833
                     /locus_tag="VEW08_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYJ39605.1"
                     /translation="MSSQNIKSVLVEERSFPPSPEFTARARLKPADAAALRREAAADH
                     VGFWARQARSELRWHKPFTHTLDDSQAPNYRWFTDGELNVSYNCLDVHLEERGHKTAV
                     TFVGEPGDVRHVSYRELHADVCRFANALLAQGVKAGDRVVIYMPLTPEAVVAMHACAR
                     IGAIHSVVFGGFSALSLRDRIEDAGAVMLITADGGWRGGKVVELKEAADKALSKGCAT
                     IRSVIVLKRTAHDVPMKNGRDLWWHDVVEGQIPVCEPHWVDAEHPLFLLYTSGSTGKP
                     KGIQHASAGYLLGAKLTSKWVFDLNDEDVFWCTADIGWVTGHSYVAYGPLAAGATIVL
                     YEGAPTTPHPGRFWQICESLGVTIFYTAPTAIRALMKLGDDIPARYDLTRLRLLGSVG
                     EPINPEAWMWYHEHVGRMRCPIVDTWWQTETGA"
BASE COUNT          653 a         1285 c         1171 g          724 t
ORIGIN      
        1 caccgccagc ttcttcttga gctcaatcat gtgatcaacg tccattcgcg gttgcgtgaa
       61 gtcgcggctg atgtcgtgcc gcccgacgat tggacttcct ggcaggacct gctagccgta
      121 ccagcgctgg catttgagca aggacgctcc tttcttgatg atctgcgggc cctcggactc
      181 aatccgatgg acagcgtctc gctgactccg ttgccgcaga ccattgccct tgccgcctat
      241 gcccactacc agctcgttga acgcgcgact gtgggcctgg tgggttacct gtggttcttc
      301 gagcgaatgc ccaggctgct ctacccgttg tggagcgagt cgtgtcgtcg cggcggagtc
      361 ccggagaagg cgctacgcgc gctggccgag ggcgcggtcg tgcacgcggt gcgtgatcag
      421 gccgtagtga actgctgccg ccagatcgtg cgccggccgc gcgacctggg gctggccacg
      481 cagagcctcc acgacaccgc ggagctgttc ggcaccatgc tggacgcggc gttgcagcgt
      541 gccgagcggc gccatccgct ggccgtggaa agtgtggtgg tggctggcgg gattggccag
      601 gtcgcctgac cagaacggtg ccaggcaccg ttttctagga aagggtgcct gacacccttt
      661 accatcctcg cgtgatctat ctcatcgacg cgtcggtcta cgtattccgc gcctactact
      721 ccatgccgcc cgacatggtc gacagcgacg gcaatcccgc gcacgccacc tttggtttcg
      781 cccgctttct cggcgatctc atcgaacgca cgcagccgcg ttatatcgcc gttgccttcg
      841 acgagagcct gacgacgtcg ttccgcaacc agatttatcc cgcttacaag gcgaaccggg
      901 atccggcgcc cgcggatctc aagctgcagt tcgcgcggtg ccgggaattc tgccggcatg
      961 cgggcatccc ggccttcagt cacgccgagt acgaagccga tgacatcgtt ggcacgctga
     1021 tgacgcgctg ccggagcgaa ggcctgcgcg cgacgctggt cacgcgcgac aaggactatg
     1081 cgcagctgat acgcaccggg gacgtgtact gggactactc cgacaacgtg cgctatcgct
     1141 acgacgaaat cgaagaccgg ttcggcgtgc agccagagcg attcgccgat ttcctcgctc
     1201 tcatgggtga cagcgtcgac aacatcaagg gcgtaccggg tgtcggggca aagaccgcgg
     1261 cggcgctcat gaaggaattc gtgtcgctcg aggagatcta cgacaacctc gatcgcgtgg
     1321 gcgatatcgc ggtgcgcggc gccggcaagc tcgccgcgaa actcatcgag cacaaggaag
     1381 ccgccttctt cgcgcgccgc ctcaccgaga tcgcctgcaa catgccgctg gaaacgacgc
     1441 gcatggatct gatacggcgc gcacccgacg tttcggccct taccgcgttc tttgaccggc
     1501 acaacttcgg gccaatgctg cgccgtcagg cggaacgtct cgccttgctg ccgctggctg
     1561 ccgccgccta gtttccgagc ctattcgacg tcgcgatcgt cgtcattgcg cttgttgttc
     1621 ggcacggtga cccttccgcc gatcgactcg tgttcgatgg accccgagct gtcttcgcgc
     1681 accgtgaagt cgccgctgac ggatccggcg tagatgtcgc ccgagctgtc ggaatccacg
     1741 ctgacgctgc ccttcacatc ttcgacgcgg atgccgccgg agctgtcctg ctccaccctc
     1801 acgttgccgc tgaccttcgc gatctcgata tcgcccgagc tgtcgagtac gacgtctaca
     1861 tcacctttca cgtcgtctac gtcgatgtcg ccggagctgt cgcgcacgcg aacgctgccc
     1921 gccgtctcga tttcgatgtc acccgagctg tcgccgacgt cggccaggcc ggcaatgcga
     1981 ttgaggttga tgtcaccgga gctgtcctgc agcgtgagcg cgttgaggtc ttcaaaggtc
     2041 gcgtcgccgc tcgagtccgt ggcttccacg ggaatgttcg acggtacagc gatgccgatg
     2101 tcgatgtagg cgtattcgtt atcgccccat gaccacttgc cgtcgtcctg cggcagcgtg
     2161 gtttcgacat acaccacgtt gccttcgcgc cgcacggaga tctgcgtggc gtccagcaat
     2221 tcctgtttgg cggcacaggc cacgccgcga gcctcgatgc gtacggcgtt gccgcgcccg
     2281 acaaccttca tgtcgcccgc gccggcgcgg atgacgactt tttcgacccc cgcagcctcg
     2341 atacccccgg cgcgatccgc ccgcaacttg cacccatcac cccaggcgag cgcggtcgcg
     2401 gggaccaggg cgagcaatac caatgggtat ctgaaacgca tgtgacttct cccgaaatgc
     2461 tgattggcta tggttaagac tcgcgagagg gcatttgggt ttacaggacc gaacaattca
     2521 tacggtatcc ttcgggccgc ccccgagcag tcaaaacatg agcagccaaa acatcaaatc
     2581 tgttctcgtc gaagaacgca gttttccgcc ttcccccgaa tttaccgccc gggcccgcct
     2641 gaagcccgcg gacgccgccg ccttgcggcg cgaagcggct gcggaccacg tgggcttctg
     2701 ggcccgccag gcgcgcagcg aacttcgctg gcacaaaccg ttcacccaca ccctggacga
     2761 ttcacaggct cccaactacc gctggttcac ggatggcgag ctcaacgttt cctacaactg
     2821 cctcgacgtc cacctcgagg agcgcggcca caagactgcg gtgaccttcg tgggcgagcc
     2881 gggcgacgtt cgccatgtct cataccgcga gctgcatgcc gatgtatgcc gcttcgccaa
     2941 tgcgctgctg gcgcaggggg tcaaggccgg ggatcgggtg gtcatctaca tgccgctcac
     3001 gcccgaagcc gtcgtcgcca tgcatgcctg cgcgcgtatc ggcgccatcc actccgtggt
     3061 attcggtgga ttctcggcgc tgtcgctgcg cgatcgcatc gaagatgccg gcgcagtcat
     3121 gctcatcacc gccgacggcg ggtggcgcgg cggcaaggtc gtcgagctca aggaagccgc
     3181 cgacaaggcg ctgtccaagg gttgcgccac catccgcagc gtcatcgtgc tcaagcgcac
     3241 cgcccacgat gtgcccatga aaaacgggcg agacctctgg tggcacgacg tggtcgaagg
     3301 ccagatcccg gtgtgcgagc cgcactgggt cgacgccgag catccgctgt tcctgctgta
     3361 cacctccggc tccaccggca aacccaaggg catccagcac gccagcgccg gctatctgct
     3421 gggcgcgaag ctcaccagca agtgggtgtt cgatctcaac gacgaagacg tgttctggtg
     3481 cacggcggac attggctggg tcaccggcca cagctatgtc gcttacggcc cattggccgc
     3541 gggcgccacc atcgtgctgt acgaaggtgc gccgaccacg ccacacccgg gacgcttctg
     3601 gcagatctgc gaatcgctgg gcgtcaccat tttttacacc gcgcccacgg cgatccgcgc
     3661 gctcatgaaa ctcggcgacg acattccggc gcgttacgac ctcacgcgtc tgcggttgct
     3721 cggatcggtg ggcgagccca tcaatcccga agcctggatg tggtaccacg agcatgtcgg
     3781 tcgcatgcgc tgcccgatcg tcgacacgtg gtggcagacc gaaacgggcg cca
//
LOCUS       DASQJS010000062         3446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1918566, whole genome shotgun sequence.
ACCESSION   DASQJS010000062 DASQJS010000000
VERSION     DASQJS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918566"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VEW08_02380"
     CDS             complement(<1..687)
                     /locus_tag="VEW08_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HYJ39606.1"
                     /translation="MAHEHTASGSHSGAHDDHAHSDDHGDHGPAKGWRRWVYATNHKD
                     IGTMYLIFACVMFFVGGFMAMIIRAELFQPGLQLVEPQFFNSMTTMHALLMIFGAVMP
                     AWTGLANWMIPMQVGAPDMALPRLNNFSFWILPFAFTLLLSTLFVPGGAPAGGWTLYP
                     PLSLQAGDGLPMAIFAIHLMGASSILGSINVVVTIMNMRAPGVGLLNMPLFCWSWLIT
                     AYLLIATMPVL"
     gene            complement(709..1599)
                     /gene="coxB"
                     /locus_tag="VEW08_02385"
     CDS             complement(709..1599)
                     /gene="coxB"
                     /locus_tag="VEW08_02385"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HYJ39607.1"
                     /translation="MSFHMKNAYARRGIKAAAIPLFALLASVAHADWGGLNMPEGVSI
                     LSKSIYGLHMKIFYYCVAIGVVVFGAMIYSLIKFRKSQGAQSDTTLVHSTKVEIIWTV
                     IPILILVAMAIPTARGLIAIEDMDGATLDIKVTGYQWKWEYEYLGHGVSFFSTLARES
                     NAARQLGSGIDPATVPNYLLDVDNPLVIPAGTKIRFLLTAQDVIHAWWVPAFGMKKDA
                     IPGYVSPIWVQVDADKVGTYRGQCAELCGQDHGFMPIVVEVKSPADYDAWLRAKQAEQ
                     KVAGESSPTAVAAVASAVNQ"
     gene            complement(1608..2111)
                     /locus_tag="VEW08_02390"
     CDS             complement(1608..2111)
                     /locus_tag="VEW08_02390"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HYJ39608.1"
                     /translation="MFDAATHIDLTPHCSLSVRGAVMFFGSVCLPTFAVAGIATALGF
                     WPVLPFAGAEMLLLAWALHSNMQRRFEHETIDISDTEVVITYSRGEPARVVFPRHWAR
                     VKIRRPNSPLHRSHLVIESHGRAYEVGKFLTEEERHQLAAALRLRIGGMNQSPALPAT
                     GSPDQFS"
     gene            complement(2146..2850)
                     /gene="bioD"
                     /locus_tag="VEW08_02395"
     CDS             complement(2146..2850)
                     /gene="bioD"
                     /locus_tag="VEW08_02395"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HYJ39609.1"
                     /translation="MTDTPAKQGFFVTGTDTGVGKTLVTVALTRAFVARGLRTAVMKP
                     VAAGIVQTSEGPRNEDALELLEQSNVSAPYEDVNPWLLTTPASPHLAARHDGVSISHE
                     RILAAHRRLAARADLLLIEGAGGWLAPISSVATMADIAEKLGLPVVFVVGLRLGCLNH
                     ALLTREAIRSSGLPFAGWIANKMAAEMPLANANIESLTSRFGVPPLAVVPPGNLASQG
                     QVPAWAVEAANKLVSA"
     gene            complement(2847..>3446)
                     /locus_tag="VEW08_02400"
     CDS             complement(2847..>3446)
                     /locus_tag="VEW08_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ39610.1"
                     /translation="RFERVRADAYSLPFRDGSFDLVYSSLMLQWCDDLDTAFGEIARV
                     LKPGGLLLFSTFGPGTLAELREAWAASGDAGNHVNHFFDPHAVGSALMHAGLSEPVLD
                     VDRIVMRYPDARALMRELKAIGAHNVTAGRPRGLTGRKRLAAMTQAYETLRGEGGLPA
                     TYEVIHAICWGGERRAQQPPDFPRESFIAPGDIRRRSQT"
BASE COUNT          652 a         1052 c         1143 g          599 t
ORIGIN      
        1 cagcaccggc atggtggcga tgagcagata ggcggtgatc agccagctcc agcagaacag
       61 cggcatgttg agcaggccca cgcccggcgc gcgcatgttc atgatcgtga cgaccacgtt
      121 gatggagccg aggatggacg aggcgcccat gaggtggatg gcgaagatcg ccatcggcag
      181 gccgtcaccc gcctgcagcg acagcggcgg atacagcgtc cagccgccgg ccggcgcgcc
      241 gcccggaacg aaaagagtgc ttaacagcag cgtgaacgcg aacggcagga tccagaacga
      301 gaagttgttg agacgcggca gcgccatgtc gggcgcgccc acctgcatcg ggatcatcca
      361 gttcgcgagg cccgtccagg ccggcatgac cgcgccgaag atcatgagca gcgcgtgcat
      421 ggtggtcatg gagttgaaga actgcggctc gacgagctgc aggccgggct ggaacagctc
      481 ggcgcggatg atcatcgcca tgaagccgcc gacgaagaac atcacgcagg cgaagatgag
      541 gtacatcgtg ccgatgtcct tgtgattcgt cgcgtagacc cagcggcgcc agcccttggc
      601 cgggccgtgg tcaccatggt catcagaatg ggcgtggtcg tcgtgtgcgc cggaatgtga
      661 accagaagct gtgtgttcgt gtgccatgtg cattatctcg tcgaaaactt attgattgac
      721 ggcggaagca acggccgcga cggccgtggg tgaagactca cccgcgacct tctgttcggc
      781 ctgcttggcg cgcagccagg cgtcgtagtc ggccggcgat ttcacttcga ccacgatggg
      841 catgaagccg tgatcctggc cgcagagttc ggcgcactgg ccgcggtacg tgccgacctt
      901 gtcggcgtcc acctggaccc agatcgggct cacgtagccg gggatggcgt ctttcttcat
      961 gccgaacgcg ggcacccacc aggcgtggat gacgtcctgc gcggtgagca ggaagcggat
     1021 cttcgtgcca gccggaatca ccagggggtt gtccacgtcg agcaggtagt taggaacggt
     1081 ggcgggatcg atgccggagc cgagctggcg tgcggcgttg ctttcgcggg ccagcgtgga
     1141 gaagaaactc acgccgtggc cgaggtactc gtattcccac ttccactggt agcccgtgac
     1201 cttgatgtcg agcgttgcgc catccatgtc ctcgatggcg atgaggccgc gggccgtggg
     1261 tatggccatg gcgaccagga tcaggatggg aatcaccgtc cagatgattt ccaccttggt
     1321 gctgtggacc agcgtggtgt cactttgggc gccctgggac ttccggaact tgatgagcga
     1381 gtagatcatc gcgccgaaca ccaccacgcc gatcgcgacg cagtagtaga agatcttcat
     1441 gtgcaggccg tagatgctct tgctgaggat ggagacaccc tcgggcatgt tgagcccgcc
     1501 ccagtcggcg tgcgcaacgg acgccagcaa ggcgaacagg gggatggcgg cggccttgat
     1561 tccacggcgc gcgtaggcat tcttcatgtg aaagctcatc gatgacctca agaaaactga
     1621 tccggtgaac cggtcgcggg cagtgccggc gactgattca tgccgccaat ccgcaaccgc
     1681 aacgcggctg ccagctggtg gcgctcttct tctgtcagaa acttgcctac ctcgtatgcc
     1741 cggccgtgcg actcgatgac gaggtggctg cggtggagcg gcgaattcgg gcggcggatt
     1801 ttaacccgtg cccagtggcg cgggaacaca actcgagcag gttcaccgcg cgaataggtg
     1861 atgaccactt cggtgtccga gatgtcgatg gtctcgtgct cgaagcgtct ttgcatattc
     1921 gagtgcagcg cccaggcgag cagcagcatt tcagcgcccg cgaacggcag gaccggccag
     1981 aaccctagag cggtggcgat gccggctacg gcgaaagtcg gcaggcacac gctgccaaaa
     2041 aacatgacag cgccgcgaac actcaacgag cagtgtggcg tcaggtcgat gtgggtcgcg
     2101 gcatcaaaca cggcggcgac tataccagcg actttgccgg cttactcaag cacttacgag
     2161 cttatttgcg gcctcgaccg cccatgctgg tacttgccct tgggatgcga gatttccggg
     2221 gggcaccacg gccagcgggg gcactccaaa gcggctcgtc agtgattcga tgttggcgtt
     2281 cgccagcggc atttccgccg ccattttatt ggcgatccat cccgcgaatg gcagtccgct
     2341 ggagcgaatg gcttcacgag tgagcaatgc gtggttgaga caaccgaggc gcaggccgac
     2401 gacgaagacc accggcaacc cgagtttttc cgcgatgtcg gccatggtcg ccacgctcga
     2461 gatgggtgcg agccagccgc cagctccctc gatgagcagc aggtccgccc tcgccgccag
     2521 gcgccgatgc gccgcgagta ttcgttcgtg actgatcgag acgccatcgt ggcgcgcggc
     2581 caggtgcggc gacgccggcg tggtcagcag ccacgggttc acatcctcgt agggtgcact
     2641 gacgttgctc tgctctaaca actcgagcgc gtcttcgtta cgcggacctt cggacgtctg
     2701 gacgatgccg gccgccaccg gcttcatgac cgccgtgcgc aggccgcgcg ccacgaatgc
     2761 gcgcgtgagc gccaccgtca ccagcgtctt gccgacgccg gtgtcggtac ccgtgacgaa
     2821 aaagccttgt tttgcaggag tatctgtcac gtctggctgc gccggcgaat gtcgccgggc
     2881 gcgatgaaac tctcgcgcgg aaaatccggc ggctgctgcg cgcggcgctc accgccccag
     2941 cagatcgcgt gaatgacttc gtaggttgcg ggcaaaccac cttcgccgcg cagcgtttca
     3001 tacgcctggg tcatcgcggc gagccgcttt cggcccgtga ggccgcgtgg acgaccggcg
     3061 gtcacgttgt gcgcgccgat ggccttgagc tcacgcatga gcgcacgcgc gtccgggtaa
     3121 cgcatgacga ttcgatcgac atcgagaacc ggttccgaaa ggccggcgtg catcaatgca
     3181 ctgccgaccg cgtgcggatc gaaaaaatga ttcacgtgat tgccggcgtc accactcgcc
     3241 gcccatgcct cgcgcaattc ggcgagcgtg ccgggcccga aggtgctgaa cagcagcagt
     3301 ccaccaggct tgagcacccg cgcgatctcg ccgaacgcgg tgtcgagatc gtcacaccat
     3361 tgcagcatca ggctgctgta gacgagatcg aaggagccgt cgcgaaatgg cagcgaatac
     3421 gcatcggcgc gcacgcgttc aaagcg
//
LOCUS       DASQJS010000063         7877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3804543, whole genome shotgun sequence.
ACCESSION   DASQJS010000063 DASQJS010000000
VERSION     DASQJS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7877
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3804543"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VEW08_02405"
     CDS             complement(<1..1463)
                     /locus_tag="VEW08_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ39611.1"
                     /translation="MYRAPVKDLRFVLDELIGVETLRACPEFAEYSTETVDAVLGEAA
                     RFAEVVLEPLCKSGDREGATWSPNGVTMPAGYKDAYRQYCENGWPALRSKVEFGGQNA
                     PAVLGTAVEELWAASNLAFKLCPMLTQGAIEAIQHFGSPQQKQLYLPKMVSGEWTGTM
                     NLTEPQAGSDLAQVRTRATPNGPNYRLYGQKIFITYGEHDLTENIVHMVLARIDGAPP
                     GVRGISLFIVPKYWVNADGAPGARNDVNCASIEHKLGIHASPTCVMMYGENEGALGLL
                     VGEPNRGLEYMFVMMNAARLSVGLEGYAQGERAFQQAVEWARTRVQGKPPVPLARISG
                     SASMGTSSSPAPIIGHPDVKRMLLSMKSTVEACRALALYAAHQLDIGAGHPDEAVRAA
                     HELRGDLLIPIVKGFCTECGIEVASVGIQVHGGMGYIEETGAAQTYRDARITSIYEGT
                     TGIQSNDLIGRKIGRDGGATLGAFAEEMQLSLRAIQT"
     gene            complement(1486..2382)
                     /locus_tag="VEW08_02410"
     CDS             complement(1486..2382)
                     /locus_tag="VEW08_02410"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYJ39612.1"
                     /translation="MSELWSAAQLAVFARVVELNGFSSAARAYGVPKVAISRAIADLE
                     KSIGLQLMRRTTRRIFLTAAGDAVLPHARAISVEVEKVRQLAQTFSTSREGPLRVIAD
                     PAYGRVLLGPLVPRFLDSFPDIALDVVLDSGEGVDWDVAIRAGVNEDPKLGARLLGAP
                     PAVLCATPAYLLKHPAPERPEGLRDHALLTPAGEGAEYRFQMTKASARAEVRVRPKLA
                     VDDPAVLHGATVAGLGIGLLPEFLCRQGVATAKLRRVLADWQAPSAAPLYAVFPAGLE
                     NDPRVRSFVDFAAANIVPALAQ"
     gene            2449..2952
                     /locus_tag="VEW08_02415"
     CDS             2449..2952
                     /locus_tag="VEW08_02415"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HYJ39613.1"
                     /translation="MKISMHAMSYDVFKRALLQLRHVMEKAVANAEARKFETGVLVGA
                     RLAPDMLPFARQVQLTSDFAKNSMARLAGIDPPKFEDTETTMDELFARVKKTIEFIDS
                     VPAASLEGSETRDIKIVLRDRTVEFKGLAFLQHWAIPNFFFHHVTAYNLLRHNGVDIG
                     KRDFLGG"
     gene            complement(3042..5390)
                     /locus_tag="VEW08_02420"
     CDS             complement(3042..5390)
                     /locus_tag="VEW08_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HYJ39614.1"
                     /translation="MAQAQAQAPTIVEAESGSLGASLTTRTDAAAGVNYITVLSAANS
                     GATPTPDRVATYQVTFPAAGSYALYVRILAGPVGGMDDSFYIPSGFNNATNWPGLYNT
                     SSGGATAAGAGVSTTGSAGQNVWKWVRLTGIPAIGDSSIGPTAWVVPDGELTQSFSWG
                     SREDGLLFDKLAFAPVNLCYTVGDLDAVRAPTVSCPPPQPTPAAYTRPPGSPLAVGQA
                     KYLGSAHSPAPTDPNQTNPSLNFGAYWNQVTPENGGKWGSAQPTSPFGPAADGYPTLP
                     NPAFNWAAARAAYQQARSTGAAFKWHTLFWGNQQPAWIETLPVAKQQEAIRIWFAAIA
                     AEFPDIEQIEVVNEPLHDPPRGPTNGNYIEALGGNGVTGWDWIIRSFELAREYFPNAK
                     LVLNDYGITNDGNATTNYLTIINLLKDRGLIDHIGIQGHAFEFNYNNLPGSAATHRAN
                     IARLAATGLPIYVTEFDIDGADSVWGLPDDVAQTARFQAFFPVFWESDAIKGVTLWGY
                     MRGSHWRTGTGAWLMYTNGAERPALQWLVRYMENKRAVVTAGQSFSVNEDVAGGTAIG
                     TVLATDTDAETTLSQWQIEADPSGKFAIDAGTGSLSLATGATLDFEMTTSYQVSVSVW
                     DGYVRSVPGTVTISVANVNDNAPAVTAGQAFDIDEGYKNKIGWLESSDPDDMNQIGFT
                     TFQGWQIVSGNTGSVFRVTPSAGELQVARPLLIDWRKTSYTLGTTVSDGALTSTATPV
                     TVVIPKRIDMCLFDIIRLEVPKASAPLVFLLGGELGSCFRPL"
     gene            5709..7604
                     /locus_tag="VEW08_02425"
     CDS             5709..7604
                     /locus_tag="VEW08_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ39615.1"
                     /translation="MLSPGKFSRMQASASVTASGDSKAAIGDALKQALSGNRLQSISL
                     HDERGDVLWLDEGALGPDEHNLVVEAAAALTAGPELTYVFQNVGDGRSAAFLPARSPL
                     NDLLGLAMIIADAKFLDSKGAAKFVTPTTTNLMRRLSFLRKPTSPAAPVNPPPAARVA
                     SPPNAAQAPRPAAQAPRPAAPPAARPGAPVAARPGAPAAARPAAPAAQRPAAPQAKAP
                     AAPARPPVPAKAPVAPPKAAQPAAKPAAPPARPAAPPAKPAVQQRPSGSADSDSIAFE
                     ISDIDPRSLLKGAQRAAQPEAKRPDLRVVQTGRMPKLELKLEADPLPELIQDEPPEQE
                     VALEPMYADDVSGEEVTTEVEALAEPDADMNVQPEFVDEVPQLSAGAGQFEFYGQIFE
                     LHAQQLVKLKGNGGTRRFEILVRDAHADNEFGVAPERVLQDVHDPSANSDLDRIVVEH
                     LLIWMNDNRGIWETDPASFSVNVGMGTIADPKFATFVAATLKNHKIPAKALGFEIPEK
                     SCVDQVRDVDMFVQACEKIGCHVVLDDFTFHHHSLRWLGSAALKFVKLDPKITAVAMK
                     ERVPQALVVAIAQASKVLGLSCVAKRVDTPAVRDWLSAVGLDYAQGFLLDQPRSLAGL
                     VAASAKK"
     gene            7675..>7877
                     /locus_tag="VEW08_02430"
     CDS             7675..>7877
                     /locus_tag="VEW08_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39616.1"
                     /translation="MSLDPLLKNRVALFWTLHIGGWLSYMLSQYLGTLLYDAKYEHMT
                     GYLTVIMIAAVSGFLLSLELRYI"
BASE COUNT         1396 a         2579 c         2492 g         1410 t
ORIGIN      
        1 tcggtctgga tcgcgcgcag cgacagttgc atctcctcgg cgaaggcgcc aagcgtggcg
       61 ccgccatcgc ggccgatctt gcggccgatc agatcgttcg actggatgcc ggtggtgccc
      121 tcgtaaatcg acgtgatgcg cgcgtcgcga tacgtctgcg cggccccggt ttcctcgatg
      181 tagcccatgc cgccgtgcac ctggatgccg accgacgcga cctcgatgcc acactcggtg
      241 cagaacccct tgacgatggg aatcagcagg tcgccgcgca actcgtgcgc cgcgcgcacc
      301 gcttcgtccg gatgcccggc gccgatgtcc agctggtgcg cggcgtacaa cgccagcgcc
      361 cggcaggcct ccaccgtgga cttcatgctc aacaacatgc gctttacgtc ggggtgaccg
      421 atgatcggcg ccggcgacga gctggtgccc atcgatgccg agcccgagat tcgcgcgagc
      481 ggcaccgggg gcttgccctg cacgcgcgtg cgcgcccact ccaccgcctg ctggaacgcg
      541 cgttcgccct gcgcgtaacc ctcgaggccg acggaaagac gtgcggcgtt catcatcacg
      601 aacatgtatt cgaggccgcg gttgggttcg ccgaccagca ggccgagtgc gccttcgttt
      661 tccccgtaca tcatcacgca ggtcgggctc gcgtggatgc cgagtttgtg ttcgatggac
      721 gcacagttca cgtcgttgcg cgcgccgggg gcgccatcgg cgttgaccca gtacttggga
      781 acgatgaaca gcgagatgcc gcgcaccccg ggcggagcgc cgtcgatgcg cgccagcacc
      841 atgtgcacga tgttctccgt caagtcgtgc tcgccgtagg tgatgaagat cttctggccg
      901 tagagacggt agttagggcc attgggcgtg gcgcgcgtgc gcacctgcgc gagatcgctg
      961 cccgcctggg gctcggtcag gttcatggtg ccggtccact cgccgctcac catcttcggc
     1021 aggtatagct gcttctgctg cggcgacccg aagtgctgga tggcttcgat ggcgccctgc
     1081 gtgagcatgg ggcacagctt gaacgcgaga ttcgaggcag cccaaagctc ttcgaccgcg
     1141 gtgccgagca cggccggcgc gttctggccc ccgaactcca ccttggaacg cagcgccggc
     1201 cagccgtttt cgcaatattg ccggtaggcg tccttgtacc ctgcgggcat ggtcacgccg
     1261 ttcggcgacc aggtcgcccc ttctctatcg ccgctcttgc agagcggttc gagcacgact
     1321 tcggcgaacc gcgcggcctc gccaagaacg gcgtcgacgg tttcggtcga gtactcggcg
     1381 aactccggac aggcgcgcaa ggtttccacg ccaatgagtt cgtccaacac aaaccggagg
     1441 tccttgaccg gtgcgcggta catcgtcact ccttaactga caggactact gggccaacgc
     1501 cggaacgatg ttcgcggcag cgaaatccac gaagctgcgg acgcggggat cgttctcgag
     1561 gccggccgga aacacggcgt acagtggcgc cgcagacgga gcctgccaat cagcgagcac
     1621 tctgcgcaac tttgccgtgg caaccccctg gcggcacagg aattcgggca gcaggccgat
     1681 gccgagcccc gcgaccgtgg cgccatgcag caccgcaggg tcatccaccg caagctttgg
     1741 gcgtacgcgc acttcggcac gggcggaagc cttggtcatc tggaagcgat attcggcgcc
     1801 ttcacccgcc ggcgtgagca gggcgtgatc gcgcaggccc tcgggccgct cgggcgccgg
     1861 atgcttgaga aggtatgcgg gagtcgcaca caagacggcg ggcggtgcgc cgagcagtcg
     1921 tgcccccagc ttcgggtctt cattgacgcc cgcacggatt gccacgtccc agtcgacgcc
     1981 ctcgccggaa tcgagcacca cgtcgagcgc gatatccgga aagctgtcga gaaatctcgg
     2041 aacgagcgga cccagcagca cccggccgta cgccggatcg gcgatcaccc gcaggggtcc
     2101 ctcgcgagag gtagagaagg tttgcgcgag ctggcgtacc ttttcgacct cgacgctgat
     2161 ggcgcgcgcg tgcggcagca ctgcatcgcc ggcagcggtc agaaaaattc gccgggtcgt
     2221 acgcctcatg agctgcaacc cgatggattt ctcgagatca gcgatggcgc ggctgatcgc
     2281 gaccttgggc acaccgtagg ccctggcggc ggaagaaaag ccattgagtt cgacgactct
     2341 cgcgaaaacg gcgagctgcg cagcggacca taattcactc ataggcgcac caaagagagc
     2401 gatggtggcc gcattgtttc actaaaggaa ttaaaaagga aagtcctcat gaaaatttcg
     2461 atgcatgcga tgagctacga cgtcttcaag cgagccttgt tgcagctccg gcacgtcatg
     2521 gaaaaagccg tcgcaaatgc cgaagcgcgc aaattcgaaa ccggcgtgct cgtgggcgcg
     2581 cggcttgccc cggacatgct gccgttcgcc cggcaggttc aactcaccag cgacttcgcg
     2641 aagaactcga tggcccggct cgcgggtatc gacccaccga aattcgaaga taccgagacg
     2701 acgatggacg agctgttcgc gcgcgtgaag aaaaccatcg aattcatcga cagcgtgccg
     2761 gcggcatcac tcgaagggag cgagacgcgc gacatcaaga ttgtgctgcg cgatcgcact
     2821 gtcgagttca aggggctcgc attcctgcag cactgggcta tcccgaactt cttcttccac
     2881 cacgtgaccg cctacaacct gctgcgtcac aacggcgtgg atatcggcaa acgtgatttt
     2941 cttggtggat aaggctgggc ggataaagct cggcggctga tttcgccggg cataaaaagg
     3001 gccggctccg cagccggccc tttttcgtcc gcgtcaggta atcacagcgg ccggaagcac
     3061 gatccgagtt cgccacccag caggaagacc agcggcgcgc tcgccttggg cacttcgagc
     3121 ctgatgatgt cgaacaggca catgtcgatc cgcttcggaa tgaccacggt cacaggcgtc
     3181 gcggtgctcg tgagggctcc atcgctcacg gtagttccca gcgtgtacga ggttttgcgc
     3241 cagtcgatca acaacggacg cgcgacctga agctcacccg ccgacggcgt gaccctgaac
     3301 accgaacccg tgttgccgct gacgatctgc caaccctgga aggtcgtgaa cccgatctgg
     3361 ttcatgtcgt ccggatcgga actctcgagc cacccgatct tgttcttgta gccctcgtcg
     3421 atatcgaagg cctggcccgc cgtgacagcc ggagcgttgt cgttcacgtt cgcgacactg
     3481 atggtcaccg tgccaggcac gctgcgaacg taaccgtccc agaccgatac gctgacctgg
     3541 taggaggtgg tcatctcgaa gtcgagggtc gcacccgtcg ccaggctcag gctgcccgtg
     3601 cccgcatcga tcgcgaactt gccgctcgga tccgcctcga tttgccactg cgacaacgtc
     3661 gtttcggcat cggtgtcggt tgcgagcacc gtgccgatcg cggttccgcc cgcgacatcc
     3721 tcgttcacgc tgaacgactg tcccgcagtg accaccgcac gcttgttttc catgtagcgc
     3781 accagccatt gcaatgccgg acgttccgcg ccgttggtgt acatgagcca tgcgccagtg
     3841 ccggtgcgcc agtgcgagcc gcgcatgtag ccccagagcg tgacgccctt gatcgcgtcg
     3901 ctctcccaga acaccgggaa aaatgcctgg aatcgcgccg tctgggccac gtcgtccggc
     3961 agcccccaga cactatcggc accgtcgatg tcgaactcgg tgacgtagat cggcaggccg
     4021 gtggccgcaa gccgcgcgat gttcgcacgg tgcgtggccg cggagcccgg cagattgttg
     4081 tagttgaact cgaacgcatg cccctggatg ccgatgtggt cgatgaggcc acgatccttg
     4141 agcaggttga tgatggtcag gtagttagtg gttgcattgc cgtcgttggt gatgccgtag
     4201 tcgttcaaca ccagtttggc attcgggaaa tactcccgcg ccagctcgaa ggatctgatg
     4261 atccagtccc agccagtgac accgttgccg cccagggctt cgatgtagtt gccattcgtg
     4321 gggccacgcg gcggatcgtg cagcggctcg ttgaccactt cgatctgctc gatatccgga
     4381 aactcggccg caatcgccgc aaaccagata cgaatcgctt cctgctgctt ggcgaccggc
     4441 agggtttcga tccaggcggg ttgctggttg ccccagaaca gggtgtgcca cttgaacgcg
     4501 gcgccggtgc tgcgcgcctg ttgataggcc gcgcgcgccg ctgcccagtt gaaggctgga
     4561 ttcggcagcg ttggatagcc atcggcggcc ggaccgaacg gtgaagtcgg ctgggccgag
     4621 ccccacttgc caccattctc cggcgtcacc tgattccagt acgcgccgaa attgagcgac
     4681 gggttcgtct gattcgggtc tgtcggtgcc gggctgtgcg cgctgccgag gtacttggcc
     4741 tggccgacgg caagtggcga accgggcggg cgcgtgtacg ccgccggagt tggttgcggc
     4801 ggcggacagc tgacggtcgg cgcgcgtacc gcgtcgagat cgcccacggt gtagcagagg
     4861 ttcacgggag caaacgccag cttgtcgaac aagagcccgt cttcgcgcga gccccaggaa
     4921 aatgactgcg tcaactcgcc atcggggacc acccaggcgg tcgggccgat gctgctgtcg
     4981 ccaattgccg ggatacccgt caggcgaacc cacttccaga cgttttgacc cgcgcttccc
     5041 gtggtggata caccggcacc ggccgcagtc gcgccgccgc tgctggtgtt gtagagacct
     5101 ggccaattgg tggcgttatt gaatccgctc gggatgtaga aactgtcgtc catgccgcca
     5161 accgggccgg ccaggatgcg cacgtacagc gcgtagctgc cggcagcggg gaacgtaacc
     5221 tggtacgtcg cgacccgatc cggtgtcgga gtggcccccg aattcgccgc ggacagaacg
     5281 gtgatgtagt tgacgcccgc ggcggcgtcg gttcgagtag ttaggctggc gccgagcgat
     5341 ccgctttctg cttcgactat ggtcggcgcc tgggcctgtg cttgcgccat cgaaacaatt
     5401 gatacgcccg cgagtagaca tgcgcgcaac agcgcgctcc ttgtgctggt catacgattc
     5461 cccttcgttt ttgagacgca gtggttagcc actgtcgcgc gtccggaatc tagccgaaga
     5521 gaaacgaatt aggtagcggt aacatccgcg aactttcgcc ctttgcctct tctccgactt
     5581 tggtagtggg tgtgaactac tgcgcgaagc gcttccatat tgaacataac gacggcaaat
     5641 cgacgttcca ccattccggg gaacgggagc tatgtcacgg cggcgcattc ggtgatttgc
     5701 gactctgcat gctcagtccg gggaaatttt cacgcatgca ggcgagcgcg agtgtcacag
     5761 ctagcggcga ttcgaaggcc gcgatcggcg atgccctgaa gcaggcgctt tccggcaatc
     5821 ggctgcaatc catttccctt cacgacgaac gcggcgatgt cctttggctc gacgagggcg
     5881 cgctgggtcc cgacgaacac aatctcgtcg tggaggctgc tgccgcgctg accgccggtc
     5941 ccgagctcac ctacgtcttc cagaacgtcg gtgacggacg ctcggctgcg ttcctgccgg
     6001 cgcgttcacc gctcaacgat ctgctcggcc tcgcgatgat catcgccgac gccaagttcc
     6061 tcgacagcaa gggcgcggcg aaattcgtca cgccgaccac cacgaatctc atgcgccggc
     6121 tctcgttcct gcgcaagccg acttccccgg ccgctcccgt caatccgccg cccgccgcgc
     6181 gagtagctag tccgcccaac gccgcgcaag cgccacgtcc cgcagcgcag gcgccccgcc
     6241 ccgccgcgcc gcccgccgca cgccccggcg caccggttgc cgcccgtccc ggcgcacccg
     6301 ctgctgcgcg tcccgccgcg ccggccgcgc agcgtccggc tgcgccgcag gcgaaggcgc
     6361 ccgccgcacc cgcacgccca ccggttcccg ccaaagcccc ggtagcgcct cccaaggctg
     6421 cgcagcccgc cgcaaagccg gctgctccgc cggcacgtcc ggccgcgccg cctgcgaagc
     6481 ctgccgtgca gcagcgcccg agcggcagcg cggattcgga ttccatcgca ttcgaaattt
     6541 ccgacatcga tccgaggtcg ttgctcaaag gtgcccagcg cgcggctcag cccgaagcga
     6601 agcgccctga cctgcgcgtg gtgcagaccg gtcgcatgcc gaagctcgag ttgaaactcg
     6661 aagcggatcc gcttcccgag ttgatccagg acgagccacc cgagcaggag gtcgcgctcg
     6721 agccgatgta cgcggacgat gtcagcggcg aggaagtgac caccgaagtc gaagcgctgg
     6781 cggagcccga cgccgacatg aatgtccagc cggagttcgt cgacgaggtg ccgcagttgt
     6841 ccgcgggcgc cggtcagttc gagttctatg gccagatttt cgagcttcac gcgcagcagc
     6901 tcgtgaagct caagggaaac ggcggcacgc gccgtttcga aatcctggtt cgcgatgcgc
     6961 atgccgacaa tgaattcggt gtggcgccgg agcgcgtcct gcaggacgtg cacgacccga
     7021 gcgccaattc ggatctcgac cgtatcgtgg tcgaacacct gctcatctgg atgaatgaca
     7081 accgcggcat ctgggagacc gaccccgcca gtttctccgt gaacgtgggc atgggcacga
     7141 tcgccgaccc gaagttcgca actttcgtgg cagcaacgct caagaaccac aagattcccg
     7201 ccaaggcgct gggcttcgag atccccgaaa aatcgtgcgt cgaccaggtc cgcgacgtcg
     7261 acatgttcgt gcaggcctgc gagaagatcg gctgtcacgt ggtgctcgac gacttcacgt
     7321 tccatcacca ctcgttgcga tggctgggtt ccgcggcgct caagttcgtc aagctcgatc
     7381 ccaagatcac cgcggtggcg atgaaagaac gcgtgccgca ggcgctggtc gtggccattg
     7441 cacaggcttc gaaagtgctc ggcctgtcct gcgtcgccaa gcgtgtggac acgccggcgg
     7501 tgcgcgactg gctctcggcc gtgggcctcg actacgcgca gggattcctg ctcgatcagc
     7561 cgcgctcgct ggccggcctg gtcgcggctt ccgccaagaa ataacgacga ggcgaggacg
     7621 ccgcgcgcca gggcgtttgt cccccatggc gacctcccgt acctttgacg gatggtgagc
     7681 ctcgatccac tgctgaaaaa ccgtgttgcg ctcttctgga cgctgcacat cggcggctgg
     7741 ctgtcgtaca tgttgtcgca gtacctgggc acgctgctgt acgacgccaa gtacgaacac
     7801 atgaccggct acctcacggt catcatgatc gccgccgtca gcggcttcct gttgagcctg
     7861 gagctgcgct acatcta
//
LOCUS       DASQJS010000064        11280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_36533, whole genome shotgun sequence.
ACCESSION   DASQJS010000064 DASQJS010000000
VERSION     DASQJS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11280
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36533"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1801)
                     /gene="sulP"
                     /locus_tag="VEW08_02435"
     CDS             complement(137..1801)
                     /gene="sulP"
                     /locus_tag="VEW08_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HYJ39617.1"
                     /translation="MLRSYEAAWLPHDLMAGLVLTTMLVPVGIAYAVASGVPGIYGLY
                     ATIVPLLAYALFGPSRILVLGPDSSLAAVILAVVLPLSAGDPMRAVIVASLMAVVSGL
                     VCILIGVMRLGFVTELLSKPIRYGYMNGIALTVLISQLPKLFGFSIEGSGPMRDVLQI
                     GQSILDGKINWAAFAVGGSTLIAILVLKRWERIPGLLIAVVGATIAVGVLGLEESAGV
                     KVLGALPQGLPSFVLPWIGFADLGQIVIGGCAVAMVAFADTSVLSRIYAAKTKSHVDP
                     NQEMVGLGAANLAAGLFQGFPISSSSSRTPVAEAAGSKTQLTGVVGAIAVALLVLLAP
                     MLLEDLPNSALAAVVIAAAIGLFEFADLRRIFRIQRWEFWLSIVCFVGVAVFGAIPGI
                     GLAILIAVLEFLWDGWRPHFAVLGRVSGIRGYHDIKRYPDARLVPGLVLFRWDAPLFF
                     ANAELFHQRLLEAVAQSPTPVKRIVVTAEPVTSIDVTSADMLAELEQTLRESGTELRF
                     AEMKDPVKDKLKRFELLERFGAANFHPTVGAAVDAYLEDHSVDWKP"
     gene            complement(1966..2388)
                     /locus_tag="VEW08_02440"
     CDS             complement(1966..2388)
                     /locus_tag="VEW08_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39618.1"
                     /translation="MGRRSLHLSALLALIAGLSACGDESNSPGERRLLSGNTVVFESR
                     GRLQCDFTGGTTPELSAMKLIREGVDVMRSGCGGMTEVAFAAVCGGPSGELVLHEIRA
                     VNLDTAERAGFRDVAELKVPGGSQGYAWVDCNTGAILP"
     gene            complement(2462..3055)
                     /locus_tag="VEW08_02445"
     CDS             complement(2462..3055)
                     /locus_tag="VEW08_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HYJ39619.1"
                     /translation="MEAKVPVDVEGLTVREGHFLLESGLHAAGWLDLDTLFLDPGTLA
                     PQIAALAALISRHQITAVCGPLVGGAFVAQALALQLGVRFYFAERVPSNPEEGLFQAQ
                     YRLPHSQRSRAATERFAVVDDVIGAGSAVRATVSELASLGATTAVVAALLTLGNKAES
                     HLTQRGIPLVALSPKEIAMWEPAICPHCQAGIPLQSV"
     gene            complement(3074..3553)
                     /locus_tag="VEW08_02450"
     CDS             complement(3074..3553)
                     /locus_tag="VEW08_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39620.1"
                     /translation="MSNVIASTLRLIRSFNRHDSAERVRSMRQIAADDPEGILAPWLA
                     AIAFWEEGEIEEARRLAEVILEKEPTDFRMLVICLDWSIRIGDPRRTLAYAERVASVE
                     RPTRRMRKINAVLSVLFWPIRLLGYGGGMRHEVKVLDAWSEYAREYVKVHGQNVAAT"
     gene            complement(3565..3987)
                     /locus_tag="VEW08_02455"
     CDS             complement(3565..3987)
                     /locus_tag="VEW08_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39621.1"
                     /translation="MRSLLQLLAICLCPERTKAPAQGLDPHAYLRPLIVLVIVVLCGP
                     EVFAAADLVALLDLLGAGLFLTAFAAGFQALGVLALTRMQQILFPAEWTVFLKAHRHP
                     SIMAHGLMLIGVNVLSISVCCLVASIWVYEVVWEAVLI"
     gene            5256..6365
                     /locus_tag="VEW08_02460"
     CDS             5256..6365
                     /locus_tag="VEW08_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYJ39622.1"
                     /translation="MEFYTKAFFVALAVLVAYGMLLVLQPFAGAMAWAIFLAFLLHPL
                     HGWLTRKFRGKAGMSAGVITGLTPFALLTPLTLLAFEFVHQARALVHFVRGSDFKLDG
                     SILMRLEQYPVIGPLARLAREELLVSAADIQEWLGRATETALRSVASIGSGVLLGALG
                     TLVAFFFMLFLLFFFLRDGTRMFDRLQRLIPVPEDHREQLFDHLASVARGVFYGIGLT
                     AICQGVLVGIAFAIAGMPSPVVFGVLAGILALLPAGGAAIVWIPAVLYLAAVGSWGMM
                     IFMLIAGVITSTADNFLRPILVSRYAPVSAFMVFVGVVGGIAAFGAIGIVVGPVFLAL
                     VAAIIEYFDEKVIAPNKRNLDAQADDERPPPMPGL"
     gene            complement(6426..7184)
                     /locus_tag="VEW08_02465"
     CDS             complement(6426..7184)
                     /locus_tag="VEW08_02465"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HYJ39623.1"
                     /translation="MFDLQDKVAVITGGNGGIGFGMAQGLATEGARIVIAARNAAKSA
                     AAVELLKSSGAQALAIEVDVTNEDSVARLFDQAHARCGRVDILINNAGINIRKPVQAL
                     ALEEWKQVVATNLDSAFLCCRAAYPHLKGAGGGKIINIGSMLSIFGAGFAPAYGASKG
                     GVVQLTKSLASAWAADNIQVNAVLPGWVNTDLTRKARVEVDGLHERVLERTPARRWGE
                     AADFAGIAIFLASAASNFITGVAIPVDGGYSSQG"
     gene            7286..9454
                     /gene="uvrD"
                     /locus_tag="VEW08_02470"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             7286..9454
                     /gene="uvrD"
                     /locus_tag="VEW08_02470"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HYJ39624.1"
                     /translation="MDLSPILDSLNEAQRDAVTAPVGPVLVLAGAGSGKTRVLTHRIA
                     WVIQTEGASPHSILAVTFTNKAAHEMRGRIEKLLGIPGSNMWVGTFHGIAHRLLRLHW
                     RDAKLPQTFQILDGEDQLRLIKKIVKAMELDEARWVPREIQYFINHNKDEGHRPPTLK
                     DGNDPTRRQLIKIYAEYEAQCQRTGVVDFAELLLRAYELWLYNPEILKHYQHRFRHVL
                     IDEFQDTNAIQYAWSLLVAGQAGAPFVVGDDDQSIYRWRGARVENLTRFSKDYPAAKL
                     YRLEQNYRSTGNILKAANALISNNTGRLGKNLWTEGKDGERIKLYAAFNERDEAEFVM
                     HRIRDWTNQGGARREVAILYRSNAQSRVFEEIFMSARIPYKVYGGLRFFERAEIKDAL
                     AYLRIITNHADDASFERVVNLPTRGIGNKSLDVVREQAKAATSSLWDAAAHCVAHGVL
                     GPKAEAAVNGFLKLVEQLGRDIDGLELHEQVDHVINNSGLVEHHKKEKADRGEARVEN
                     LNELVSAARGFEPDGTNTDDEALPPLDAFLSHAVLESGEGQAEAWEDCIQMMTLHTAK
                     GLEFPVVFLCGLEDGLFPHQRSLNDEAGLEEERRLCYVGTTRAMKQLYLTYAEQRRLH
                     GQDNYGTPSRFISEFPEDLVEEVRPRIQISRPVAAGRFKPPTEELAPGVRLGARVRHK
                     KFGEGVILKVEGHGPQANIQVNFASLGVKIMMLEYLEVVR"
     gene            9451..10263
                     /gene="pyrF"
                     /locus_tag="VEW08_02475"
     CDS             9451..10263
                     /gene="pyrF"
                     /locus_tag="VEW08_02475"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HYJ39625.1"
                     /translation="MNFSEKLAAAWKATNSLVCVGLDPEPKKFPDQFRDAPGGIFQFN
                     RAIIDATRDLVCAYKPQFAHYAAHAAENQLEHTIDYIKSTCPNAVVILDSKRGDIGST
                     AEQYAHEAFERYDADAVTVNPYLGRDSVEPFLKHADKGVIILCRTSNPGAKDFQDLDV
                     GGRKLFQHVAEQVAKEWNTANNCMLVVGATYPEELADIRQRVGDLPFLVPGVGAQGGD
                     VAKVMAAGKTAAGTGLVISSSRAILYASSGEDFASAARKAAQSLRDSINSHR"
     gene            complement(10323..10796)
                     /locus_tag="VEW08_02480"
     CDS             complement(10323..10796)
                     /locus_tag="VEW08_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39626.1"
                     /translation="MKHRVGLGLTLAVCAAVEAAEPSSSPVPTASVPAAAPAASPTPA
                     PATPVPVPTSKPLPAKPLDLRVGDVRKYMMPNEYRAVLDAPHAEKNTVVVEGKRELLP
                     VEYEEPIPVGPLMPLWWGLKNPSQSWRIFLPDLNRTERPREPEDKVPPPIFRWGP"
     gene            complement(10918..>11280)
                     /locus_tag="VEW08_02485"
     CDS             complement(10918..>11280)
                     /locus_tag="VEW08_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39627.1"
                     /translation="TFVRAEAEIVRRQTDILTFPPNARVNRLEIAQRLRHEVLEPWRA
                     ASRPLLQATTLPQDDSRSSRMQEALRDYVRARASAIELRALAYESGEPADEARANLAE
                     KQLGRTLNVVNSLANERS"
BASE COUNT         2213 a         3483 c         3525 g         2059 t
ORIGIN      
        1 cctcatccgt aacttcaccc aggaaaacca tgggttcgac gcccggcatc gtgaggtcac
       61 gatccgcgaa cgtgagacgg aagcaggtgc ggccgatgac cagcgatgac ggcttcatgt
      121 gttttcttgt cgatccttaa ggtttccaat ccaccgagtg atcttcgagg taggcatcga
      181 cggcagctcc gacagtcgga tggaagttgg cggcaccgaa tcgctcgagc agctcgaagc
      241 gcttcagttt gtctttgacc gggtccttca tttccgcgaa gcgcaactcg gttcccgatt
      301 cgcgcagggt ctgctcgagt tccgcgagca tgtctgccga agtcacatcg atgctggtga
      361 ccggttcggc ggtcacaacg attcgcttta ctggtgtcgg cgattgcgcc acggcctcga
      421 gcaggcgctg gtggaacagc tcggcattgg cgaagaagag cggcgcatcc caacggaaca
      481 gcaccaggcc gggtacgagc cgcgcatccg gataacgttt gatgtcgtgg tagccgcgga
      541 tgccgctgac tcggccgagc acggcgaagt gcggccgcca cccgtcccat agaaactcga
      601 ggaccgcgat gaggatcgcc aggccgatgc ctggaatcgc gccgaaaacg gcgacgccca
      661 cgaagcagac gatagatagc cagaactccc aacgctggat gcggaaaatg cggcgcaggt
      721 cggcgaactc gaacaggcca atcgcagccg cgatcacgac ggctgccagc gcgctgttgg
      781 gtaagtcttc cagcaacatg ggcgccagca gcaccagcag cgcgacggcg atcgcgccga
      841 ccacgccggt gagctgcgtc ttcgagcctg cggcctcggc caccggcgtg cgcgaggaac
      901 tgctgctgat cggaaagccc tggaacaggc cagcggcaag attggcggcg cccagtccga
      961 ccatttcctg gttcggatcg acgtgggact tggtcttggc cgcatatatg cgcgacagca
     1021 cgctggtgtc ggcgaacgcc accatggcga cggcgcaacc gccgatgacg atttgaccga
     1081 ggtcggcgaa accgatccac ggcagtacga aactcggcaa gccctggggt agcgcaccga
     1141 gcaccttcac tccggcgctt tcttccaggc caagtacgcc gacagcgatc gtggcgccca
     1201 cgacggcgat caggagtccc ggaattcgct cccatcgctt gagcaccagg atcgcgatca
     1261 gcgtgctgcc gccgaccgcg aacgctgccc aattgatttt tccgtcgagg atcgactggc
     1321 cgatctgaag cacgtcgcgc atcggtccgc tgccttcgat cgaaaagcca aacagcttgg
     1381 gtagctggct gatcagcacg gtgagtgcaa tgccgttcat gtatccatag cgaatcggct
     1441 tcgacagcag ctcggtgacg aaccctaagc gcatgacgcc gatcagtatg cacaccagcc
     1501 ccgacaccac cgccatcagg ctggcgacga tcacggcgcg cattggatcg cccgcggaca
     1561 gaggcaacac aactgcgagg atcacggcgg cgagcgagga atccggcccg agtaccagaa
     1621 tgcggctcgg gccgaacagt gcataagcga gcagcgggac gatggtggca tacagcccgt
     1681 agattcccgg gacgcctgag gcgacggcat aagcaatgcc gaccggcacc agcatggtgg
     1741 tcagcaccag ccccgccatc agatcgtgcg gcagccacgc ggcttcgtac gatctgagca
     1801 tttgcaatcc gggcagccaa cgcttccagc cgttcacgtt ccgtcccttg aatggagcgc
     1861 aatcatgcgc ggtccaacag tttgttagac gtctccattc gaatagacca catgcggtta
     1921 tgcgtggtca aaccgtcgcc cagggcaggc ggcgcggagg gcagttcagg gcaggattgc
     1981 gcccgtgttg cagtcaaccc acgcgtagcc ctggctgcca cctggaacct tcagctccgc
     2041 gacatcgcgg aaacccgcgc gttcggcggt gtccagattc acggccctga tctcgtgcaa
     2101 cacgagttcg ccagatggac cgccgcatac tgccgcgaag gccacttcag tcatcccgcc
     2161 gcatccggag cgcatgacgt cgacaccttc gcggatcagc ttcatcgcgc tcagctccgg
     2221 cgtcgtgccg ccagtgaagt cgcactgcag tcttccgcgg ctctcgaaaa cgacggtgtt
     2281 gcccgatagc agccgcctct cgccgggcga gttcgactca tcgccacagg ctgacaggcc
     2341 ggcaatcagc gcgagtagcg cagagaggtg aagtgagcgt cgtcccatga acatgggtgg
     2401 tacggtaaat ccgctgctca gagcgtgtgt ataggaaatg taggcaacgg tctcgaactg
     2461 gttagaccga ttgcagtgga attccggctt ggcaatgcgg acagatagcc ggttcccaca
     2521 tggcgatttc cttcgggctc aacgcgacaa gcggaatgcc gcgttgtgtc aaatgacttt
     2581 cggctttgtt tccaagagta agcagtgcgg cgacgaccgc ggtcgtcgcg ccaaggctcg
     2641 ccagttcgct caccgtggcg cggaccgcag atccggcgcc tatgacatcg tcgaccaccg
     2701 cgaaacgttc cgtagcggca cggctacgct gtgaatgtgg cagccggtat tgagcctgga
     2761 agagtccctc ttcgggattg gacgggactc gctccgcaaa atagaaccga acgccgagct
     2821 gaagcgcgag cgcttgagca acgaatgcgc cgcccaccaa agggccgcac accgccgtga
     2881 tctggtgcct ggatatgagc gcggcgagcg ccgctatctg aggcgcgagt gttccggggt
     2941 caagaaacaa tgtgtcgaga tcgagccagc ccgcggcgtg cagccccgac tcgagcagga
     3001 agtgaccctc gcgaacggtt agtccttcga catcgactgg cactttagct tccacgcgcg
     3061 ctccggcgag gtgtcaggtc gcagcaacgt tctggccatg gactttcaca tactctcgcg
     3121 cgtattcgct ccacgcgtcg agcaccttca cctcatgccg cattccgccg ccgtagccga
     3181 gcagacgaat tggccagaag agcaccgaca gcaccgcatt gattttgcgc atccgccgtg
     3241 ttggtcgctc cacgctcgct acacgttctg cataggcaag cgtccggcgt gggtctccga
     3301 tacgaatgct ccagtccagg cagattacga gcattcggaa atctgtcggc tccttctcga
     3361 ggatgacctc agccaggcgg cgagcttctt ctatctcccc ttcctcccag aaggcgatag
     3421 cagccagcca cggagcaagg atgccttctg gatcatctgc tgcgatttgg cgcatggaac
     3481 gcacacgttc cgcgctgtcg tggcgattga agctgcgtat gaggcgcaaa gtagatgcga
     3541 tgacgttgga catgcgaccg cttgttagat cagaaccgct tcccacacaa cttcgtagac
     3601 ccagatagat gcaaccaaac agcacaccga aatactcaga acattcacgc cgataagcat
     3661 gaggccgtgc gccataattg aagggtgtcg atgggccttt agaaaaacag tccactcagc
     3721 cggaaaaagt atctgctgca tccgcgtaag ggccaagacg ccaagggcct gaaatcccgc
     3781 cgcaaatgcc gtcagaaaga gccccgcgcc gaggagatcg agcaacgcga ccaggtcggc
     3841 agccgcgaac acttctggtc cgcagagcac aacaatgacc aggacaatga gtggcctcag
     3901 ataggcatga ggatcgaggc cttgcgcagg cgctttagtg cgctctggac acaggcagat
     3961 agccaacaat tgcagcaggc ttcgcatagt gtcaggtggt gagctcattg cggacgaacg
     4021 aaagttcaat ctcgtgaata cggtcatccg cgatgccgat ggtcaacatg acacgcctcg
     4081 cagggagcca gaggcaaacc acctgtcttt gcgccaataa acggatttgc tcggcatcgc
     4141 ttgcgacaac gtccgactcg cgaacggtga gagggggagc acctttctct ctgccacacc
     4201 cggcctgcac caagcctgat gcaccatatt gcgaaacgat tgtgcggacg agttcgtcac
     4261 gacttgcgcc taccttgaag cctaatacgt cgccatcctg gtacgtcgaa aaatagttga
     4321 accatggcgg ccgaaacaag gaagcccgct ccggggattc gcgagctacg cgatgagggt
     4381 aagacgagaa tgcgacggag gcataaaccg cggcgaccag gagcacgacg aggatgaggg
     4441 ccggtcggat tccgcgactc actaccgtca cctatcggtt gggctgagcg gcgcccgcgt
     4501 tgtggaccgg gcttggcatt ctatttcgca cgcgtccgct ccaataattt gttaggtggc
     4561 caccacactc agagtacctt ttgcatgaac cactcaactg gcgagtagag cccaacgttc
     4621 aggatcatga agagcaccca caatgccacg aatagcgtac agatcactat gactcgcgcg
     4681 tgtagccccc accgagctcc actgaacatg acgatgccac tcgcactggc taacggcacc
     4741 aaccatatgg cgcgtccgat cgtaagacct agtgtcgtcc acgtggagag cggcggtgga
     4801 ttgctcactt ccgccatcag cttttgcacg ggacggagat ccgcgccgat gcgccatgtg
     4861 agaaaacata gcgatacggt caggaaaagg ccggacactc cgaaaagcat tctggaatct
     4921 cgcgagtcgc tcacagtgac cacctaactc ttaactagac cgcataggtg cggcaaaact
     4981 atccgtcgat tctgccgcat cagatttcag cctcgccgca actttctgtt tcgactacag
     5041 catcgttccg acttgtcgtc aaactactcc gacgtgtttg tggcaagtcg tataacagaa
     5101 tgccttcccg ctcctgtcag gcgaacgatg cccgaacccc caatcccggc gatgcctccc
     5161 ttcccgccct cacgtcctcc taacccgcta tagacccggc tctcccgaca catatataag
     5221 agcacccaac aaagccttcc ttcttaaggg tgcccgtgga gttctataca aaagccttct
     5281 tcgtcgcgct cgccgtgctg gtggcgtacg gcatgttgtt ggtgctccag cccttcgcgg
     5341 gtgcaatggc ttgggcaata ttccttgcct tcctgctcca tccgctgcac ggctggctga
     5401 ctcgcaagtt tcgcggcaag gcgggcatgt cggcgggtgt aatcacgggt ctcacgccct
     5461 tcgcgctgct gacgccgctc accttgctgg ccttcgagtt cgtgcatcag gcccgcgcgc
     5521 tggtgcactt cgtgcgcggc agcgatttca agctggatgg cagcatcctc atgcgcctcg
     5581 agcagtaccc tgtgattggc ccgttggcgc ggctcgcgcg tgaagaactg ttggtgtcgg
     5641 cagcggacat ccaggaatgg ctggggcgcg ccacggaaac ggcactacgc agcgtggcgt
     5701 cgatcggtag cggcgtgctg ctgggagctc ttggcacgct ggtggcgttt ttcttcatgc
     5761 ttttcctgct gttcttcttc cttcgcgacg gcacgcggat gttcgaccgg ctgcagcggc
     5821 tgatcccggt gcccgaggac catcgcgaac aactctttga tcacctggca agcgtggcgc
     5881 gcggcgtttt ctacggcatc gggctgacgg cgatctgcca gggcgtcctt gtgggaatcg
     5941 cgttcgcgat tgcggggatg ccgtcgccgg tggtgttcgg tgtgctcgcg ggaattctgg
     6001 cgctgctgcc ggcgggtggc gcggccatcg tatggattcc ggcggtgctg tatctggccg
     6061 ccgtcggctc ctggggaatg atgattttca tgctcatcgc cggcgtcatc acgtccaccg
     6121 cggacaattt cctgcggccc atcctggtgt cgcgttacgc gccggtgtcg gcgttcatgg
     6181 tgttcgtcgg cgtggtgggc ggcattgcag ccttcggcgc catcggcatc gtggtgggac
     6241 cggtcttcct cgcgctggtg gcggcaatca tcgaatactt cgacgagaag gtcattgcgc
     6301 cgaacaagcg caacctcgat gcgcaggcgg acgacgagcg gccgccgccg atgccggggc
     6361 tctgaaatgg aaatggggtc aggcaccgat tgctaggaat cggtgcctga ccccgttttc
     6421 aatcctcagc cctgggagga atagccgccg tcgaccggaa tcgcgacgcc ggtaatgaag
     6481 ttcgatgcgg cgctcgccag aaatatggca atgccggcga aatcggcggc ttcgccccag
     6541 cgtcgcgctg gtgtgcgctc gaggacgcgc tcatgcaggc cgtcgacttc cacgcgcgcc
     6601 ttgcgcgtga gatcggtatt cacccagccg ggcagcaccg cgttcacctg aatgttgtcc
     6661 gcggcccacg cggatgcgag tgatttcgtg agctgcacga cgccgccctt gctcgcgcca
     6721 taggccggcg caaaccccgc gccgaagatg gagagcatgg acccgatatt gatgatcttt
     6781 ccgccaccgg cgcccttcag gtgcggatac gcagcgcggc agcagagaaa tgcgctgtcg
     6841 agattggtcg cgacgacctg tttccattcc tcgagtgcca gcgcctgaac gggcttgcgg
     6901 atgttgattc cggcattgtt gatgaggatg tcgacgcgac cgcaacgggc gtgtgcctgg
     6961 tcaaagaggc gcgcgacgga gtcttcgttg gtcacgtcga cttcgatggc cagcgcctgc
     7021 gcgcccgagg atttcagcag ctccacggcc gccgcggatt ttgccgcgtt acgcgccgca
     7081 atgacgatgc gtgcgccctc cgtggcgagc ccctgcgcca tgccgaatcc gatgccaccg
     7141 ttgccacccg tgatgactgc gaccttgtct tgcaagtcga acatcggtga attccttctg
     7201 gtttcgcgcg catcttacgc ccctctgatg tcggcaaaac acgcggccac gtgcccgcgc
     7261 gcctcgccta taatccgcgc cctccatgga cctctccccc attctggatt cgctcaacga
     7321 ggcgcaacgc gacgcggtca ccgcgcccgt gggcccggtg ctggtgctgg ccggcgcggg
     7381 ctccggcaaa acgcgcgtgc tcacgcatcg catcgcctgg gtcatccaga ccgagggcgc
     7441 gtcgccgcat tccatcctcg cggtcacgtt cacgaacaag gccgcgcacg agatgcgcgg
     7501 gcgcatcgag aagttgttag gcattccggg aagcaacatg tgggtgggca cgttccacgg
     7561 catcgcgcac cggctgctgc gcctgcactg gcgcgatgcc aagctcccgc agaccttcca
     7621 gattctcgat ggcgaagacc agctgcggct catcaagaag atcgtgaagg ccatggagct
     7681 cgacgaagcg cgctgggttc cgcgcgagat ccagtacttc atcaatcaca acaaggatga
     7741 aggccatcgc ccgccgacgc tgaaggatgg caacgacccg acgcgccggc agctcatcaa
     7801 gatctacgcc gagtatgaag cccagtgcca gcgcacgggc gtggtggatt tcgcggagct
     7861 gctgctgcgc gcctacgaac tgtggttgta caacccggag attctcaagc actaccagca
     7921 tcgcttccgg cacgtgctca tcgatgagtt ccaggacacg aacgccatcc agtacgcgtg
     7981 gtcgctgctg gtggcgggcc aggccggagc gccgttcgtg gtgggcgacg acgaccagtc
     8041 catctaccgc tggcgcggcg cgcgcgtgga gaacctcacg cggttttcca aggactatcc
     8101 ggcggcgaag ctgtatcgcc tcgagcagaa ctaccgctcc acgggcaaca ttctgaaggc
     8161 ggcgaacgcg ctgatctcga acaacaccgg acgcctgggc aagaacctgt ggaccgaagg
     8221 caaggacggc gaacgcatca agctgtacgc cgcgttcaac gagcgcgatg aagccgagtt
     8281 cgtgatgcat cgcatccgcg actggaccaa ccagggcggt gcgcggcgcg aggtggcgat
     8341 tctctatcgc tcgaacgcgc aatcacgtgt gttcgaagaa atcttcatgt cggcgcgcat
     8401 tccgtacaag gtgtacggcg gcctgcggtt cttcgaacgc gcggaaatca aggatgcgct
     8461 ggcctacctg cgcatcatta cgaaccatgc cgacgatgcg tcgttcgagc gcgttgtgaa
     8521 cctgccgacg cgcggcatcg gcaacaagtc gctggatgtg gtgcgcgaac aggcgaaggc
     8581 ggccacctcg tcgctgtggg acgccgcggc gcattgcgtg gctcatggcg tgctgggccc
     8641 caaggccgaa gccgcggtca acggtttcct gaagttggta gaacaactcg ggcgggacat
     8701 cgacggtctc gagctgcacg agcaggttga ccacgtcatc aacaactcgg ggctggtcga
     8761 gcaccacaag aaggagaagg cggatcgcgg cgaagcgcgc gtcgagaacc tgaacgagct
     8821 cgtgtcggcc gcgcgcggtt tcgagcccga tggcacgaac acggatgatg aggcactgcc
     8881 gccgctcgac gcgttcctgt cgcacgcggt gctggaatcc ggcgagggcc aggccgaagc
     8941 ctgggaagac tgcatccaga tgatgacgct gcatacggcc aagggactcg agttccccgt
     9001 ggtgttcctg tgcggcctgg aagacggcct gtttccgcat cagcgttcgc tgaatgatga
     9061 ggcgggtctc gaggaagagc gccggctttg ctacgtgggc acgacacgcg cgatgaaaca
     9121 gttgtacctc acctacgccg agcagcgtcg tttgcacggg caggacaact acggcacgcc
     9181 gtcgcggttc atctccgagt tcccggagga cctggttgaa gaagtgcgtc cgcgaatcca
     9241 gatttcgcgg cccgtggcgg cgggacgatt caagccgccg accgaagaac tggcgccggg
     9301 cgtgaggctg ggcgcgcgcg tgcgacacaa gaagtttggc gaaggcgtga tcctgaaggt
     9361 ggaaggccac ggaccacagg ccaacatcca ggtgaatttt gcgtcgctcg gcgtcaagat
     9421 catgatgctg gaatatctcg aggtggttag gtgaactttt ctgagaagct ggccgcggcc
     9481 tggaaagcca cgaattccct ggtgtgcgtg gggctcgatc cggagccgaa gaagtttccg
     9541 gaccagttcc gcgatgcgcc gggcggcatc ttccagttca accgcgccat catcgacgcc
     9601 acgcgcgatc tggtgtgtgc gtacaagccg cagttcgcgc attacgcggc gcacgcggca
     9661 gagaatcagc tcgagcacac catcgactac atcaaatcga cctgcccgaa tgcggtggtg
     9721 atcctcgatt cgaagcgtgg cgacatcggc agcacggcgg agcaatacgc gcacgaggcg
     9781 ttcgagcgct acgacgccga cgcggtaacg gtgaatccgt atctaggccg tgactccgtc
     9841 gaaccgttcc taaagcatgc cgacaagggc gtcatcattc tttgccgaac gagcaatccg
     9901 ggcgcgaagg attttcagga tctggacgtg ggcggcagga agctgttcca gcacgtggcc
     9961 gagcaggtgg cgaaggaatg gaacacggct aacaactgca tgctggtggt gggcgccacg
    10021 tatcccgaag agctcgccga tattcgccag cgcgtgggtg atctgccgtt cctggtgccg
    10081 ggtgtcggcg cgcagggcgg cgatgtggcg aaggtgatgg cggcgggcaa gactgcggcc
    10141 ggaacgggac tggtgatcag ttcatcacgc gcaattctct atgcgagctc gggtgaggat
    10201 tttgcgagcg ccgcgcgcaa ggccgcgcag agtctgcgcg actcgatcaa ctcgcatcgg
    10261 taggtgggtg ccgggtgggg aaagtgggga gtgcgactcc ccactttccc cacccggcac
    10321 cgtcaggggc cccagcggaa gataggcggc ggcaccttgt cctccggctc tcgtggcctc
    10381 tcagtgcgat tcaggtctgg caggaaaatc ctccaactct gcgagggatt cttgagcccc
    10441 caccacagcg gcatcaacgg tccgacagga atgggctcct cgtactcaac cggcagcaat
    10501 tcgcgcttgc cctcgaccac caccgtgttc ttctcggcat ggggcgcatc gagcaccgca
    10561 cgatattcat tcggcatcat gtatttgcga acgtcgccga cgcgcaggtc gagtggcttc
    10621 gcaggcaggg gcttggaggt cggaaccggc actggcgttg ccggggcggg agtcggagag
    10681 gcagccgggg cggctgcggg cactgacgct gtgggaaccg gcgaggacga cggctcggcg
    10741 gcttcgactg ccgcacacac cgccaacgtc agccccaaac ccacacgatg cttcatgtca
    10801 ccgcctccta acctgaagct aggaccttgc cctggaagct tagttcgacg acgcctgggg
    10861 cgcgtcccct tttgctaaca aatctggaac ggccccgaag cggggcctga ccccatttca
    10921 ggatcgttcg ttggccagtg aattgaccac gttcagggtc ctgcccagct gtttctcggc
    10981 cagattggcg cgagcctcat cggcaggttc gccggactcg taggccagcg cccgcagctc
    11041 gatggcgctt gcgcgggcgc gcacatagtc gcggagcgct tcctgcatgc gcgaggagcg
    11101 cgaatcgtcc tgcggaagcg tggtggcttg cagcagcggc cgcgacgcgg cgcgccaggg
    11161 ctcgagcact tcgtggcgca gtcgctgggc aatctccagc cgattcacgc gtgcgttggg
    11221 tggaaacgtg aggatgtcgg tctggcgacg cacgatctcc gcttccgcgc ggacgaaggt
//
LOCUS       DASQJS010000065         3832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1293826, whole genome shotgun sequence.
ACCESSION   DASQJS010000065 DASQJS010000000
VERSION     DASQJS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293826"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VEW08_02490"
     CDS             complement(<1..416)
                     /locus_tag="VEW08_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39628.1"
                     /translation="MNTRANGIASRLLAWHSKKGRHDLPWQKDRSAYRVWVSEIMLQQ
                     TQVTTVIPYFDRFMQRFPDVRALANATVDEVMHLWTGLGYYARARNLHRAAQVIRDER
                     RGIFPTGLDAVMALPGIGRSTAGAILAISAGSRHPIL"
     gene            complement(413..1630)
                     /locus_tag="VEW08_02495"
     CDS             complement(413..1630)
                     /locus_tag="VEW08_02495"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HYJ39629.1"
                     /translation="MRPLKWVSLGLGALVALVVLGVLVVVWLVDPNSFKSQIEAAVRD
                     RTGREFTLVGDIELGFFPWLSLRTGEGKFGSPADFGPEPLVSWKSAKLGVKLLPLLSG
                     DVIADRIFLSGADLRLVRRADGSANWLGLGGAQPADPNATSTQLNVDGLEIENSHLLF
                     IDESIPRRVEITGFNLSTDEISLGQPFTDTEIAGVLHMGGFVAEGVPFRLEVPKVVVP
                     EDFASVDAERFSIAFGGFEATGGVHGTLGEHPKLAGQVTAKPFDARALLAAVGIEAPK
                     TTDPKALGKIGFSGAWAFDDGAIRVDPLSFDLDDTHFAGSFSRTAGNDALGEFALSGS
                     SIDIARYIPPSDPASEPFVLPTAELKALRFRGAIHLEQATLDDIDMKDVTLRLLLDEQ
                     GLRSSPKPPAVTP"
     gene            complement(1684..2580)
                     /locus_tag="VEW08_02500"
     CDS             complement(1684..2580)
                     /locus_tag="VEW08_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HYJ39630.1"
                     /translation="MFRHSKHATALLVACALPLTAFADNDSIDRKVAADAKGEVVISN
                     VSGSIDVRGWDRNEVQVTGHLGEDVERLDVESSGGRTVVKVILPRGSSHDGDADIEVS
                     VPRNSSVEVSAVSADVSSRGVLGTQRLKSVSGEVTADISGDESEVRSVSGDVTVRGGG
                     KPARLRVSSVSGNLDLTNAAGSLDVVTVSGDANVEMGDTSDVRGRTTSGSLEVRGKLT
                     RDGRVDVEGVSGEIILRLSAPGGLSTEIESFSGDIDGCLADNVERVSKYGPGKRLNIR
                     TVESGARVRAKTLSGDIDICDR"
     gene            complement(2584..3120)
                     /locus_tag="VEW08_02505"
     CDS             complement(2584..3120)
                     /locus_tag="VEW08_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39631.1"
                     /translation="MNVRRVKRIDELAQSLPPARDLWPAIAAAIEADRAAATVDSAPR
                     RTRWLPAAGMAAAVALVTVGIFIGRSFSPPQLAAGPASTQGSLEMLPAALRDADYRKQ
                     REVLLVEVDKRLAAMPAAEREKVAASLRTLKRSISDIEAALGRDPANALLQELLVNSC
                     QEEMRALTVVRDSGSQET"
     gene            complement(3117..3704)
                     /locus_tag="VEW08_02510"
     CDS             complement(3117..3704)
                     /locus_tag="VEW08_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ39632.1"
                     /translation="MADGATARMTIADKGRADTVVDFDVALIERARKGDSKAFERLYR
                     DHVGRVHGLCLRMTRNPDLAADCAQDAFIKAWKALPRFEARSSFSTWLHRIAVNTVLE
                     KRRGPSNAEIAVEDPTEYEEPVLSFDTPVEEEELEAAIQSLPQGARDALVLCGVYGYE
                     HSEASEMLGIAVGTCKAQLHRARGLLRDRLERGVR"
BASE COUNT          667 a         1284 c         1169 g          712 t
ORIGIN      
        1 agtatgggat gccggctgcc cgctgagatc gcgagaatgg ccccggccgt cgagcggccg
       61 attcccggca gcgccatcac cgcgtcgagt cccgtgggaa atattccgcg gcgttcatcg
      121 cgaatcacct gcgccgcacg atgcagattg cgcgctcgcg cgtaatagcc gagcccggtc
      181 cacaggtgca tcacttcatc cacggtggcg ttcgccagcg cccgcacatc ggggaagcgc
      241 tgcatgaagc ggtcgaaata cgggataacc gtggtcacct gagtctgctg caacatgatt
      301 tcgctgaccc acacgcgata ggcgctgcga tctttctgcc acggcaggtc gtgacgacct
      361 ttttttgaat gccacgccag cagtcgcgag gcaattccgt ttgctcgcgt gttcatggcg
      421 tcaccgcggg tggttttggt gagctgcgca gaccctgctc gtcgagcaac agccgaagcg
      481 ttacatcttt catatcgatg tcgtcgagcg tcgcctgttc gaggtggatt gccccgcgaa
      541 agcgcaacgc tttcaattcg gccgtgggca gtacgaaggg ctcgctcgcc gggtcgctcg
      601 gcggaatgta gcgagcgatg tcgatggagg agccggaaag cgcaaattcg ccgagcgcat
      661 cgttgcccgc cgtccggctg aatgatccgg cgaaatgcgt gtcatcgaga tcaaatgaaa
      721 gcggatcgac gcggatcgcg ccatcgtcaa aagcccacgc accgctgaag ccgatcttcc
      781 cgagtgcctt cgggtcggtg gttttcggtg cttcgattcc cacggccgcc agcagcgcac
      841 gcgcgtcgaa tggtttggcc gtcacctgac ccgcaagttt tgggtgctcg ccgagcgtgc
      901 cgtgaacgcc gcccgttgcc tcgaagccgc cgaatgcgat ggagaagcgc tccgcatcga
      961 ccgacgcaaa atcttccggc actacgacct tgggcacctc gagcctgaac ggcacacctt
     1021 cggccacgaa gccgcccatg tgcagcactc cggcgatttc ggtatccgtg aatggctggc
     1081 ccagtgagat ctcgtcggta ctcaggttga aaccggtgat ttcgacgcgc cggggaatgc
     1141 tctcgtcgat gaacaacagg tggctgttct cgatttcgag gccgtcgaca ttcaactgcg
     1201 tggacgtggc attaggatct gccggctgag cgccgcccaa ccccagccag ttcgcactac
     1261 cgtccgcgcg tcttaccagg cgaaggtcgg cgccgctcag gaagatgcgg tccgcaatta
     1321 cgtccccact cagcagcgga aggagtttca cgcccagttt cgcgctcttc catgagacca
     1381 gcggttctgg cccgaagtcc gccgggctgc cgaacttgcc ttcacccgtg cgcaagctga
     1441 gccacggaaa gaagccgagc tcgatgtcac cgaccagtgt gaattcgcgg ccggtgcggt
     1501 cacgcacagc cgcttcgatt tgcgacttga agctgttggg gtcgaccagc cacacgacca
     1561 ccagcacgcc gagtacgacg agggcgacca gcgcaccgag acccagcgaa acccatttga
     1621 gaggacgcac gcggccgaca ttgtatcgat ctcgaccgcg cgggtttcgg ccgcgttaag
     1681 gcttcagcgg tcgcagatgt cgatgtcgcc cgacagcgtt ttcgcacgga cgcgcgcgcc
     1741 cgactcgacg gtgcggatgt tcagccgctt gccgggcccg tatttgctga cccgctccac
     1801 gttatcggcc aggcaaccgt cgatgtcacc gctgaaggac tcgatttccg tcgacaatcc
     1861 gcccggcgcc gacagccgca ggatgatttc cccgctgacg ccctcgacgt cgacgcgtcc
     1921 gtcgcgcgtg agcttgccgc gcacttcgag gctgcccgag gtggtgcggc cacgcacatc
     1981 gctggtgtct cccatctcca cgttggcatc gccgctgacc gtgaccacgt cgagcgaacc
     2041 cgccgcgttg gtcaggtcga ggttgccgct caccgaggac acccgcaggc gcgccggctt
     2101 gccgccaccg cgcaccgtca catcaccgct gacggagcgc acttccgatt catcaccgga
     2161 aatgtcggcc gtgacctcgc cgctgacgct cttgagtcgc tgcgtcccga gcactccgcg
     2221 cgatgacacg tcggcgctca ccgccgacac ttcgacgctg ctgttcctag gcaccgatac
     2281 ttcgatatcc gcgtcgccat cgtgcgaact gcctcgtggc aatatcacct tgaccactgt
     2341 gcgtccgccg ctcgactcca cgtcgagacg ttcgacgtcc tcacccaggt ggccggtgac
     2401 ctggacttcg ttgcggtccc agccgcgcac gtcgatggac cccgacacat tcgagatcac
     2461 gacttcgcct ttggcgtcgg ccgcgacctt gcggtcgatg gaatcgttgt cggcgaatgc
     2521 cgtgagcggc agcgcgcagg caaccagcag tgcggtggca tgttttgaat gtctgaacat
     2581 ttctcaagtc tcctggctgc cggagtcacg caccacggtc agtgcgcgca tttcctcctg
     2641 gcaggaattc actaacaact cttgcagcag cgcgttggcc gggtcgcgcc cgagcgccgc
     2701 ttcgatatcg ctgatcgaac gcttgagcgt ccgcaggctc gccgctactt tttcgcgttc
     2761 ggctgccggc atggccgcca gccgcttgtc gacttcgacc agcaggactt cgcgctgctt
     2821 gcggtaatcc gcgtcacgca gcgcggcggg caacatctcg agtgagccct gcgtcgaggc
     2881 ggggccggca gccaactgcg gaggcgagaa gctgcggcca atgaagatgc cgaccgtgac
     2941 cagcgccacc gccgccgcca tgccggccgc gggcaaccag cgggtacggc gcggagcgct
     3001 gtcgacggtg gcggccgccc ggtcggcttc gatggccgcc gcgatggcag gccacagatc
     3061 gcgcgccggc ggcaggcttt gtgccagctc atcgattcgc ttcacgcgtc gaacgttcat
     3121 cgcacacctc gttcaagtct gtcccgcaac aggcctcgag cccgatgcag ctgcgccttg
     3181 caggtcccca cggcgatgcc gagcatctcc gaggcttccg aatgttcgta accgtagaca
     3241 ccgcacagca ccagcgcatc gcgggcaccc tgcggcagcg attgaatcgc cgcctcgagc
     3301 tcttcctctt ccaccggcgt atcgaaactc aacaccggct cctcatactc ggtcggatct
     3361 tcgaccgcga tttccgcgtt cgaggggccc cgccgcttct cgagcacggt attcaccgca
     3421 atacggtgca accaggtgga gaagctgctg cgcgcctcga atctcggcag tgccttccag
     3481 gccttgatga aagcatcctg ggcacagtcg gccgccagat ccgggttgcg cgtcatgcgc
     3541 aggcaaagcc cgtgcacgcg acccacgtgg tcgcggtaga gacgttcgaa ggcctttgaa
     3601 tcgcccttcc gtgcgcgctc gatgagcgcc acgtcgaaat cgacgaccgt gtcggccctg
     3661 cctttgtcag cgatcgtcat ccgggctgtc gcgccatctg ccaaacgtct ctccctcgta
     3721 tgtaccttag aggacgtgta ggcggcatgg gtttgaaatg ccggtgccgg gccgggaaac
     3781 cgggaaaact ccggccggag cgcgacaatt cggcgaaatg actgggagtt tt
//
LOCUS       DASQJS010000066         9456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2237277, whole genome shotgun sequence.
ACCESSION   DASQJS010000066 DASQJS010000000
VERSION     DASQJS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237277"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VEW08_02515"
     CDS             <1..742
                     /locus_tag="VEW08_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide lyase family 1 protein"
                     /protein_id="HYJ39633.1"
                     /translation="VLDQIGLHIRNSSNIIIRNLHIKNVKKSGSPTSNGGDSIGMETS
                     VNNIWIDHNTLEASGGESDGYDSLLDMKAGVTNVTVSYNHYRNSSRAGLIGSSDSDSA
                     NTNITFHHNWYENIEQRTPLLRHGLAHSYNNYFSNLSNSDMIHGINSRMGGRILVEGN
                     YFKNSNNPLIASDDSDEPGCWQTRSNFLDSISYDRSVGNGALVVPIISGGQFDSTCTV
                     TVPYSYTLDSASGLPAALPGQVGVGKIQ"
     gene            889..2631
                     /locus_tag="VEW08_02520"
     CDS             889..2631
                     /locus_tag="VEW08_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HYJ39634.1"
                     /translation="MKHPFVYVLAVALLAAVPASAVVPGFAGNITGGGNATPVVVNNL
                     SAAQTAVNNYSGSGGLVLHYNGTFDEQAILNNICGQWSKPAQELSISGKNNITILGID
                     GSKANFGIRIKGTSNNIIVRNMKIGLLPGGASNGDLIGIEANSHHVWIDHNELYTRNM
                     KCEGTPDDDTTFDGMLDIKNSASFITVSYNYMHDHAKVGLIGASDTDSAERRVTYAHN
                     RYENLGSRLPFQRFGYVHSYNNWFNNITGSGINPRMGGHILVENNFFENALNPVFSQS
                     TVLGQWDLRNNNTRSSADNATYNIRWTACSGSTPCQKATNWTTTATFPITLPYSYTTY
                     PAATSKCIALNAAGAGKGLKEAPDVLSLCGGTNNLTVGSSSVSFGAGASSSPVSVTAN
                     VSWTVTDDQSWLSTTPTSGTNNGSFAVNATANTGTASRNGTVTVVGGGITRTVAVAQA
                     GTATTTSVYQTESGTVGGGTVLESSNTGFNGSGYVNASATGGFSQNSNVDGRGGGSKT
                     LRIRYALGVTAARTGRLVVNGTATNITFNGTGAWTTWALQNVTVTLNNNTSNTIRFES
                     TGQDLANIDQIEIL"
     gene            2716..3615
                     /locus_tag="VEW08_02525"
     CDS             2716..3615
                     /locus_tag="VEW08_02525"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HYJ39635.1"
                     /translation="MAGNRPPGYKTICTENKTCTVAGPIWVAFGRADKFFSKQMSGTF
                     VCNQITFGGRISGGTNECSVPIDGVSPPPPPPPAPPPPPPPPPPPQGGEITGSTCVST
                     GEVTVSQTIVVSAGTYDGGCRTYNPTLALGDGGQAEGQQPVFRIENGATVRNVIIGLN
                     GADGIHFYRGGHINNVRWLNVGEDAFTVKEGTVNQPVTITNITGVNAQDKFGQINAST
                     TMTIRNCVIDDAGKAIRQNGGTSFTAHVTMELCQLSNIRDQAFRSDSSSSTMQFRNSR
                     ITRTEELCAGSWASCTVTDVVTF"
     gene            3774..4184
                     /locus_tag="VEW08_02530"
     CDS             3774..4184
                     /locus_tag="VEW08_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYJ39636.1"
                     /translation="MVPFKLRLTPGQSIFDQVVFAAVKSILAGELKAGEPFPSVRTLA
                     LELKIHPNTAHKVVQHLIQERWLTMQPGVGTVVALPPKARAGDRKRLLEDEVERLVVE
                     AMRVGAGLGEVQQAVEDTWTDLRSLRTVAVSNGK"
     gene            4181..5080
                     /locus_tag="VEW08_02535"
     CDS             4181..5080
                     /locus_tag="VEW08_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ39637.1"
                     /translation="MIIQTNHVAKRFGRFEVIEDLNLRVPEGSVFALIGPNGTGKTTT
                     IRMLMNILRPDRGDIVVLGKPSHNLGAKDFERIGYVSEGQTLPEGLTLAQYFDYLRSI
                     YPSWDRALENELCIHFELPPTRRIKHLSHGMRMKALLVGALAFRPRLLVLDEPLSGLD
                     TLVRDEVVNGLLQQAADTTILISSHELSEIESFTTHVAFMQDGKLLLQQAIDDLRLRF
                     REVLVTLSAAKEPALALPGDWLLPESEGHRLRFIASNYQNDGALLQQLTERFGAVRME
                     CEPMPLRAIANSLMQHRKRNLKR"
     gene            5077..7590
                     /locus_tag="VEW08_02540"
     CDS             5077..7590
                     /locus_tag="VEW08_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ39638.1"
                     /translation="MNLSILGAILKKDVRSLLPLIALIALLFLADALILRLDLLPLWE
                     IWGTMVLLMAFVVLIISVFQLDSAASLTEDWLCRPLRKRELLGAKVLLVLAVAYLPHA
                     IGTVIADVSLGYPVSEVLLDAVLLPDELFLIFMPVMMFVAIITRTFVQAFGVLFTIFI
                     CVFVVPTPFLRPPGPLDSGIFDELLTSGMIWLGLAPARLASFVLAALGFWLVYWRRNL
                     LLARALMVATVFVIVFSFVLPMVLIPRKSTFALLAAFGPAPPADAARISLRSTRICFP
                     AAPRASQSTDVAFLAARNGAQLWGDEALGNSGPNSIAFLTAIEARGLPLDWRVKLNYV
                     QADYSAGGAPLESLRPATYITDDRGGGALAHSWMLPESTLRKLRNVQPQLQLTYSLTL
                     LKPREHSLPTDGKRHTLPGLGWCSAKVDEPGNRIDVDCFSAITHPAQISAQLNEISAS
                     RVYDSVNFAPAWAQQPYSHRLELAIGSPGLAKHDTITVTAWEAAGFLDKSLTLPGILG
                     ADLDTCPLPTERRDLQQARWRDPASHEAHSITVDQGVQLEVLDFGGTGSPVLLLPGLG
                     ATAHSYDELAPLLARHHRVVAMTRRGAGYSSKPDFGFDTPRLAQDVLRVMDAMRLERV
                     VLVGHSIAGDELTWLGGHHPDRFKGIIYLDAAYDRSGDRDAPDAVRLRELGRFLPLEP
                     PIPPQAMQNFDAMTKMLLERGHVPLPEGELIAFRRVNDPALAGIPNVEGRAQQAIAAA
                     IEAPDYAAVKIPALAIYAIESPNKPLPSWYDANDEEQIAYFAERARILDAMKRESIEL
                     FRHNVIEGQVLEMQNASHYIIQSNQQEVLEAIETFVAGL"
     gene            complement(7591..>9456)
                     /locus_tag="VEW08_02545"
     CDS             complement(7591..>9456)
                     /locus_tag="VEW08_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HYJ39639.1"
                     /translation="VFTATATLSQVTPMTPDAIEKYEQTLPSADFIRRDAMVPMRDGV
                     KLYTVTVMKKGTANGPMLLSRTPYDAHKATHRVASQRIVDILEVMDAEFVNDGYIRVY
                     QDIRGLHRSEGAWVLNRPLAGPVNDTGIDESTDAYDTIDWLVKNTPESNGNVGVIGSS
                     YLGFTTLMAEINPHPALKAAVPQSPMVDGWMGDDWFHNGAFRVSSLDYIVGLSTKKGD
                     GDGDFALGGGDHYTYYLEAGSVADHAKTLGIENITGVRKFLENPAYTEFWSLQAVDKW
                     LAAHPLKVPTLLEVGQWDQEDSYGAPAVYRAMEPKDKNNDMVSLHIGPWRHSGANHYG
                     YDLGALTFTGDTAREWRVEYVKPFFDHWLKGGPDPKTPPVLTYATGVNKWNVSPRWPM
                     GKAKPIYLAAGGVASFERPKAGGHEEYVSDPAKPVPFIPRPIDMNDSTQWKPWLIRDQ
                     RFVTDRPDVASWKSAPLDKAVHIMGAPEVELFASTTGTDSDWVVKLIDVYPNDVPEPA
                     SQGAKPGMAGYELPIGIEIFRGRYLKSFAKPSPLKAGKVEHYRWALPDVDHVFLPGHR
                     IMVQIQSSLFPLYDRNPQTYVENIMFARPGDYKKATQSVWFGGATATAVVLPVVP"
BASE COUNT         1824 a         3076 c         2864 g         1692 t
ORIGIN      
        1 cgtgctggac cagatcggcc tgcacatccg caactcgtcc aacatcatca tccgcaatct
       61 gcacatcaag aacgtgaaga agagcggttc gcccacctcg aacggcggcg attcgatcgg
      121 catggagact tcggtcaaca acatctggat cgaccacaac acgctggaag ccagcggtgg
      181 tgaatccgat ggttatgact cgctgctgga catgaaggcc ggtgtcacca acgtgacggt
      241 gtcgtacaac cactatcgca attcgagccg cgcgggcctc atcggctcga gcgacagcga
      301 cagtgccaac acgaacatca cgttccacca caactggtac gagaacatcg agcagcgcac
      361 accgctgctg cgccatgggc tcgcgcacag ctacaacaat tacttctcca acctcagcaa
      421 cagcgacatg atccatggca tcaattcgcg catgggtggc cgcattcttg tcgaaggcaa
      481 ttacttcaag aattcgaaca acccgctgat tgcctcggat gactcggatg agccgggatg
      541 ctggcagacg cggtcgaatt tcctggattc catttcctat gaccgttcgg tgggcaacgg
      601 cgcgctggtg gttccgatca tcagcggcgg gcagttcgac tcgacctgca cggtgaccgt
      661 gccgtacagc tacacgctgg actcggcctc gggcctgccc gcagccctgc cgggccaggt
      721 cggcgtcggg aagatccagt agtccgctgc aggtagatag atcgacaacc acagacggcc
      781 ccgttcgagg caactcgcgc ggggccgttc tcggtgccgg gtggggaaag tggggagtca
      841 ctccccactt tccccacccg gcaccaacaa gccacaagga gtgaggcgat gaaacatcca
      901 ttcgtatacg tgctggcggt agcgctgttg gcagccgtgc cggcgagcgc agtcgtgccc
      961 ggttttgcgg gaaacatcac tggcggcggc aatgcaacgc ccgtcgtcgt caacaacctg
     1021 tcggccgcgc aaaccgcggt caataactac tcgggcagcg gcggcctggt gctccactac
     1081 aacggcacgt tcgacgagca ggccatcctg aacaacatct gtggccagtg gagcaagccg
     1141 gcgcaggagt tgtcgatcag cggaaagaac aacatcacga tccttggcat cgacggctcg
     1201 aaagcgaact tcggcatccg catcaagggc acgtcgaaca acatcatcgt gcgcaatatg
     1261 aagatcggcc tgctgccggg cggcgccagc aacggcgacc tcatcggcat cgaggccaac
     1321 tcgcaccacg tgtggatcga ccacaacgaa ctgtacacgc ggaacatgaa gtgcgaaggc
     1381 acgccggacg acgacacgac gttcgacggc atgctcgaca tcaagaactc ggccagcttc
     1441 atcacggtct cgtacaacta catgcacgac cacgcgaaag tggggctgat cggcgcaagc
     1501 gacaccgaca gcgccgagcg ccgcgtcacc tatgcgcaca accgctacga aaacctgggc
     1561 tcgcgcctgc cgttccagcg cttcggctac gtgcactcgt acaacaactg gttcaacaac
     1621 atcaccggca gcggcatcaa cccgcgcatg ggtggccaca tactcgtcga gaacaacttc
     1681 ttcgagaacg cgctcaaccc ggtgttctcg cagtcgacgg tgctcggcca atgggacctg
     1741 cgcaacaaca acacgcgcag ctctgcggac aacgccacct acaacatccg ctggacggcc
     1801 tgctccggca gcactccgtg ccagaaggcg accaactgga cgacgaccgc gacgtttccg
     1861 atcacgctgc cgtactcgta caccacctat ccggcggcta cgtcgaagtg cattgcgctg
     1921 aacgcggcgg gcgcgggcaa aggcctgaag gaagcccccg acgtgctgag cctgtgcggt
     1981 ggcactaaca acctcaccgt cggatcctcg tcggtgtcgt tcggcgccgg tgcatcgtcg
     2041 tcgccggtct cggtcaccgc gaacgtgagc tggaccgtca ccgacgacca gagctggctc
     2101 agcaccacgc cgacgagcgg caccaacaac ggcagcttcg cggtgaatgc cacggccaac
     2161 accggcacgg cgagccgcaa tggcaccgta acggtggtcg gtggcggcat cacgcgcacg
     2221 gtcgcggtgg cgcaggcggg cacggccacg acgaccagtg tctatcagac cgagagcggc
     2281 accgtcggcg gcggcacggt gctcgagagc agcaacaccg ggttcaacgg ctcgggctac
     2341 gtgaatgcgt cggccaccgg cggcttctct cagaacagca acgtggatgg tcgcggcggc
     2401 ggctcgaaga cgctgcgcat ccggtacgcg ctcggcgtga cggcggcgcg cacgggccgc
     2461 ctggtcgtga acggaaccgc gacgaacatc acgttcaacg gaaccggcgc gtggaccacg
     2521 tgggcgctgc agaacgtcac cgtgacgctc aacaacaaca cgtcgaacac gatccgcttc
     2581 gaatcgactg gccaggacct ggcaaacatc gaccagatcg agattcttta gtcagtagtg
     2641 gaaacaaaag gagcgaacat ggattttcgc agcattgttt ttggattgct cgcagggacg
     2701 ttggcgatga gtgcaatggc cgggaaccgc ccgcctggct acaagaccat ctgtacggaa
     2761 aacaagacgt gtaccgtggc cggccccatc tgggtcgcct tcggtcgcgc ggacaagttc
     2821 ttctccaaac agatgtccgg cacattcgtc tgcaaccaga tcaccttcgg tgggcggatc
     2881 agcggaggca cgaacgagtg ctcggtgccg atcgatggag tgtcacctcc accgccccca
     2941 ccgccagccc cgccgccgcc gcccccgcca ccgccgccgc cccagggtgg tgagatcacc
     3001 ggttcgacgt gtgtgtccac gggcgaagtc accgtcagcc agacgattgt tgtgtcagct
     3061 ggaacgtatg acggcggatg ccgcacgtac aacccgacgc tggcgctcgg cgatggtggc
     3121 caggcagaag ggcaacaacc ggtgttccgc atcgagaatg gcgcgacggt gcgcaatgtg
     3181 atcatcgggt tgaacggtgc cgatggcatc cacttctacc gcggtggcca catcaacaat
     3241 gtgcgctggc tgaacgtcgg cgaagacgcg ttcaccgtca aggaaggcac ggtgaatcag
     3301 ccggtgacca tcaccaacat caccggcgtg aacgcgcagg acaagttcgg gcagatcaat
     3361 gcctcgacca cgatgaccat tcgcaactgc gtgatcgacg atgccggcaa ggccatccgc
     3421 cagaacggcg gcacgagctt cactgcgcac gtcacgatgg agctctgcca gttgagcaac
     3481 attcgcgacc aggcgttccg cagcgacagt tcgagcagca ccatgcagtt caggaacagc
     3541 cgcatcaccc gcaccgaaga actctgcgcg ggcagctggg cgtcctgcac cgtcaccgac
     3601 gtagtgacgt tctgaaatgg ggacattcct cgtttcctag caaacgggga caccccccaa
     3661 cgcgccccgt ccgcaaggac ggggccgcga ctccttcgat aatcggtgcg agtgcggacg
     3721 gtttaccaac gccttcccat gtgtcacagt attcatgtga caccggaggc cccatggttc
     3781 cattcaaact cagactcacc cctggccagt ccatctttga ccaggtggtc ttcgccgccg
     3841 tcaaatccat cctggccggc gaactgaaag ccggtgaacc cttcccctcc gtgcgcaccc
     3901 tcgcgctgga gctgaagatc catccgaata ccgcccacaa ggtcgtgcag cacctgatcc
     3961 aggaacgctg gctcacgatg caaccgggtg tcggaaccgt ggtggcgctg ccgccgaaag
     4021 cacgcgccgg cgaccgcaag cgcctgctcg aagacgaagt ggaaaggctg gtggtggaag
     4081 ccatgcgcgt tggcgcaggc ctcggagaag tgcagcaggc ggtggaggac acctggaccg
     4141 atctgcggag cctcaggacc gtggccgtga gtaatggcaa gtgatcatcc agaccaatca
     4201 tgtcgccaag cgattcgggc gtttcgaggt catcgaagac ctgaacctgc gcgtacccga
     4261 aggctcggtg tttgcgctga tcggccccaa tggcacgggc aagaccacca ccatccgcat
     4321 gctgatgaac atcctgcgtc ccgatcgcgg cgacatcgtc gtgctcggca agccgtcgca
     4381 caatctgggg gcgaaggact tcgagcgcat cggctacgtt tcagaaggcc agacactccc
     4441 cgaaggcctc acgcttgcgc agtatttcga ctacctgcga agcatttatc ccagctggga
     4501 ccgcgcgctc gaaaacgagc tgtgcataca cttcgagctg ccgccaacgc gcaggatcaa
     4561 acacctctca cacggaatgc gcatgaaggc tctgctcgtc ggcgcgctgg cgtttcgtcc
     4621 caggctgctg gtgctggatg aaccgctgag cggtctcgac acgctggtcc gcgacgaagt
     4681 ggtgaacggc ctgctccagc aggccgccga caccaccatc ctgatttcct cgcacgagct
     4741 ctcggagatc gagagcttca ccacgcacgt ggcgttcatg caggacggca agctgttgct
     4801 gcagcaggcc atcgacgacc tgcggctgcg cttccgcgaa gtgctggtca ctctctcggc
     4861 ggcgaaggag ccggcgctcg cgctgcccgg cgactggttg cttccggaat cggaaggtca
     4921 ccgcctgcgc ttcatcgcat ccaactacca gaacgacggc gcgctgcttc agcagctcac
     4981 ggagcgcttc ggggcggtgc gaatggaatg cgagcccatg cccctgcgcg cgatcgcaaa
     5041 ctcgctcatg cagcaccgca agcggaatct caagcgatga acctgtcgat cctcggcgcc
     5101 atcctgaaga aggatgtgcg gagcctcttg ccgctgatag ccctcattgc cctgctcttc
     5161 ctggccgatg cgctcatcct tcgcctggat ctgctgccgt tgtgggagat ctggggcacg
     5221 atggtgctgc tgatggcgtt cgtcgtgctg atcatctcgg tgtttcaact cgattcggcc
     5281 gcgagtctca ccgaagactg gctgtgccgg cccctgcgca aacgcgaatt gctcggcgcg
     5341 aaggtgctgc tcgttctggc cgtcgcctat ctacctcacg ccatcggcac cgtcatcgcc
     5401 gatgtgagtc ttggctaccc cgtttcggaa gtgctgctgg acgcggtgtt gttgccggac
     5461 gagctgttcc tgatcttcat gccggtcatg atgttcgtcg ccatcatcac gcgcactttc
     5521 gtccaggcgt tcggcgtgtt gttcaccatc ttcatctgcg tgttcgtggt tccaacgcct
     5581 ttcctgcgcc cgcctggtcc gttggattcc gggattttcg acgaactgct cacctccggc
     5641 atgatctggc tgggactcgc gcccgcacgg ctggcgtcgt tcgtgctcgc cgcgctcgga
     5701 ttctggctcg tttactggcg ccggaacctg ttgctggcgc gggccctgat ggtggccacc
     5761 gtcttcgtca tcgtgttctc gtttgtttta ccgatggtct tgataccgcg gaaatccacg
     5821 ttcgcgttgc ttgcggcatt cggcccggcg ccaccggcgg acgcggcgcg catttccttg
     5881 cgcagcacgc gcatctgctt tcccgccgca cctcgcgcga gtcaatcgac cgatgtggcg
     5941 ttcctcgccg cccggaacgg cgcccagcta tggggtgatg aggcgttggg caactcggga
     6001 cccaactcga ttgccttcct gacggcaatc gaagcgcggg gacttccgct ggattggcgc
     6061 gtcaagctca actacgtgca ggcggactat tcggccggcg gcgcgcctct cgaatcgctg
     6121 cgacccgcca cctacatcac cgacgaccgc ggcggcggag cactggcgca tagctggatg
     6181 ctgccggaga gcacgctgcg aaagcttcgg aacgtgcaac ctcaacttca gctcacgtat
     6241 tcgttgacgc tgctgaagcc gcgcgagcac agccttccca cggatggcaa gcgccacacg
     6301 ctgccgggac tcggttggtg cagcgccaag gtcgacgagc cgggcaaccg catcgacgtc
     6361 gactgcttca gtgcgattac gcatcccgcg cagatcagcg cgcagttgaa cgaaatctct
     6421 gcgagtcggg tctacgacag cgtcaacttc gcgcccgcat gggcacaaca gccatacagc
     6481 catcgcctcg agctcgcgat cggctcgccg ggtcttgcga agcacgacac catcaccgtg
     6541 actgcttggg aagccgcggg ctttctcgac aaatcgctga cgctgcccgg catcctcggt
     6601 gcagatctcg acacctgccc gctgccgacg gagcgcaggg atcttcagca ggcccgctgg
     6661 cgcgacccgg cgtcgcacga agcgcactcc atcacggtcg atcaaggcgt gcagctcgag
     6721 gtgcttgatt tcggcggcac gggctcaccc gtcttgctgc taccgggtct cggagcgacg
     6781 gcgcactcgt acgacgagct cgcgccgcta ctcgcgcgcc atcaccgcgt ggtcgcgatg
     6841 acgcgccgcg gcgccggcta ctcgagcaag cccgacttcg gcttcgacac gccgcggctc
     6901 gcgcaggacg tgctgcgcgt gatggatgcg atgcgcctcg agagagtggt gctggtgggc
     6961 cactccatcg ccggcgacga gctcacctgg ctcggcgggc atcatccgga tcgctttaaa
     7021 ggcatcatct atctggacgc cgcgtacgat cgatcgggcg atcgagatgc tccggacgcg
     7081 gtgcgcctgc gtgagctcgg ccggttcctg ccgctggaac cgccgatccc gccacaggcg
     7141 atgcagaatt tcgatgcgat gacgaagatg ctgctcgagc gcggccacgt accacttccc
     7201 gagggcgagc tcattgcgtt ccggcgcgtg aacgatccag ctcttgccgg aataccgaac
     7261 gtcgaagggc gcgcacagca ggcgatcgcc gcggcgatcg aggcgcccga ctatgcggcg
     7321 gtgaagattc cagcgctggc gatctatgcg atcgagagtc cgaacaagcc gctgccgtcc
     7381 tggtacgacg cgaacgacga ggaacagatc gcatacttcg cggagcgcgc ccgcattctc
     7441 gatgccatga agcgcgagag catcgaacta ttccggcaca acgtcattga gggacaggtg
     7501 ctcgaaatgc agaacgcgtc gcactacatc atccagtcga accagcagga agtgctggaa
     7561 gccatcgaga cgttcgttgc ggggctttag tcagggcaca actggcaaca ccaccgcggt
     7621 cgccgtggcg ccgccgaacc atacgctctg cgtcgctttc ttatagtcac ccggcctggc
     7681 aaacatgatg ttctcgacat acgtctgcgg attgcgatcg tagagcggga agaggctcga
     7741 ctggatctgc accatgatcc ggtgacccgg caggaacacg tggtcgacgt cgggcagcgc
     7801 ccaacgatag tgctccactt tgccggcttt gagcggagag ggtttggcaa agctcttcaa
     7861 ataacgtccg cggaaaatct cgatgccgat cggcagctca taaccagcca tgccgggctt
     7921 cgcgccctgc gacgcaggct ccggcacatc gttcgggtag acgtcaatca gtttcaccac
     7981 ccagtcgctg tccgtgccgg tggttgaagc aaacaactcg acctcgggcg cccccatgat
     8041 gtgaaccgcc ttgtcgagcg gtgcactctt ccagctcgcc acgtcgggac ggtccgtcac
     8101 gaaccgctga tcgcgaatca gccacggctt ccactgcgtc gagtcgttca tgtcgatcgg
     8161 gcgcgggatg aacggcaccg gcttggcggg gtcgctgacg tattcctcgt gaccgcccgc
     8221 cttcggacgc tcgaaagagg ccacgccgcc cgccgccagg tagataggct tcgccttgcc
     8281 catcggccag cgcggggaga cgttccactt gttcacgccc gtggcgtaag tgagcaccgg
     8341 cggcgtcttc ggatcggggc cgcccttcag ccaatggtcg aagaacggct tcacgtactc
     8401 cacgcgccat tcgcgcgccg tatccccggt gaacgtcagc gcgccgagat cgtagccata
     8461 gtggttggca cccgagtgcc gccagggacc gatgtgcagc gacaccatgt cgttgttctt
     8521 gtccttcggc tccatcgcgc gatagaccgc cggcgcgccg tagctgtctt cctggtccca
     8581 ctgccctacc tcgagcagcg tcggcacttt cagcggatgc gccgcgagcc atttgtccac
     8641 cgcctgcagc gaccagaatt ccgtgtacgc cgggttctcg agaaacttgc gaacaccggt
     8701 gatgttttcg atgcccagcg tcttcgcgtg atccgcgacc gagcccgcct cgaggtaata
     8761 cgtgtagtga tcgcccccgc cgagcgcgaa gtcaccgtca ccatcgccct tcttggtgga
     8821 caggccgacg atgtagtcga gcgagctgac acgaaaagcg ccgttgtgga accagtcgtc
     8881 gcccatccag ccatcgacca tcggactttg cggcaccgcc gccttgagcg cgggatgcgg
     8941 gttgatttcc gccatcagag tcgtgaaccc gagataggac gaaccgatga cgccgacatt
     9001 tccgttcgac tccggcgtgt tttttaccag ccagtcgatg gtgtcgtacg cgtcggtcga
     9061 ttcgtcgatg cccgtatcgt tgaccggtcc cgccaacggc ctgttgagca cccatgcgcc
     9121 ttcggagcga tgcaggccgc gaatgtcctg gtagacgcga atgtagccat cgttgacgaa
     9181 ctcggcgtcc atcacttcga ggatatcgac gatgcgctgg ctcgcgacac gatgcgtcgc
     9241 cttgtgcgcg tcataagggg tgcgcgacaa caacatcggg ccgttcgcgg ttcccttttt
     9301 catcacggtg accgtgtaga gcttgacgcc atcacgcatc ggcaccatcg catcgcgacg
     9361 gatgaagtct gccgatggca gagtctgttc gtacttctcg atggcgtcgg gcgtcatcgg
     9421 cgttacttgt gagagggtgg ccgtggcggt gaatac
//
LOCUS       DASQJS010000067        22160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_39391, whole genome shotgun sequence.
ACCESSION   DASQJS010000067 DASQJS010000000
VERSION     DASQJS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 22160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22160
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39391"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2943
                     /locus_tag="VEW08_02550"
     CDS             <1..2943
                     /locus_tag="VEW08_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ39640.1"
                     /translation="AGTEIDEVIVTGFRGSLQTAAAEKRANVNFTDSIFAEDIGKFPD
                     LNLAESLQRIPGVQIARDFTGEGTSVAVRGLGRDFTQITLNGAPVETASDSNIDSVSQ
                     GRGIDLVLFPTELFRQLTVSKTPTAKQQEGAVAANIDLRVARPFDDKGFHVNYSAKGS
                     FQESSEEWSPRLGFYASNTWDTGMGEFGILGGVAYSNRSYRSDGFNTIGFTTVSLGNR
                     CLYATDPQCNSFVTPNNDTGYGNQAQAWPTTVPAGYSFGANGANAGDPLTVCGAGDTA
                     GGTSNLSCRDLSFAIWPRLARPDMLIGERETTSGIITMQWAPSDKLEFYIDSLISKAD
                     HGYERNDLNLAVRSTNTNVPVNVVLNEDNIVTSATIANPVWLNENRPYHEDTDFLNLN
                     GGMKWQIGEKFTVDASINYNHSDWFRSTNTYLFNSNLNTGITVDLEANGDGVIKVTPS
                     RDLNDLTFWNWNALRVQPVKRELWQKGARVGGEWAFADAFKLGAGFSRDTFHREITNW
                     ETTSCATDGGNTTSPSGRECAAALAAAGIQHAKVAIPNAQLRNYMQAWTHGALYQTSD
                     FDVGVNNGWALPNYSLLDPATNLDFFEKELAVGLVGGLNVAGYNPRVLDEDTDAFYLE
                     ANGQLEMLGNLRYTLGARYIKTDQFVSGYISANTPDGAVRTYVEDAADYDKVLPSLNV
                     ASELGHGLVLRVAASETMTRAQPGDIAPNQSLSINGDVLTIGNAALTPYFSQNIDLGL
                     EWYFGDSGLGMIAVNAWTKEIEGYTTIVGTVTPFGQLGIDYSTLPIATQTGIQNTANA
                     QCGCTNGNPANANVRVDQRQNTSELITLDGWELTYVQPLDFLLQGAGFTVNYTHIDQE
                     SEGGLPGAPSSAITGLSPFTYNIAAFYENYGFSGRLTYSVRDAFVEFLGNNENNIAGD
                     NYAQDRKYLDASFSYKLPMDTDISISLEVQNILNEQLLTYFRSDEFTPRASFAPGRQM
                     LLGISGSF"
     gene            complement(3024..3971)
                     /locus_tag="VEW08_02555"
     CDS             complement(3024..3971)
                     /locus_tag="VEW08_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HYJ39641.1"
                     /translation="MTTRREVFPLLAGAAGASAASDSQLAAAAAPAHTALSEPELKRI
                     SYQSERTGKQRDYFVYLPRGFAQSKQWPVMLFLHGNGERGDARKDLDYVLVHGPMFEA
                     WCQRRNLPFVIISPQLPMFGQGDVDYIRNRTRDQIPQRLANWTNPRPDGRVLRLAEAM
                     QGKLSDDKLPDGPEGPPDGWNEIENELLAMVDRTIADFKGDAKRVYLTGLSYGGFGAW
                     FLAARHPEKFAALAPIVGYGHPDHAGPIARAKLPLWVFAGGRDPVVPLRFFYPALNKL
                     EELGHPEVRFTVAEELGHLAWVRIYEGQDLYSWMLAQSR"
     gene            complement(3992..5098)
                     /locus_tag="VEW08_02560"
     CDS             complement(3992..5098)
                     /locus_tag="VEW08_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HYJ39642.1"
                     /translation="MTDNVLKIRSAAETRWDCASFGEIMLRFDPGFGRVRNARTFQVW
                     EGGGEYNVARAMRKCWGKRSTAVTALPQNDLGWLVEDLMMQGGVDTSKIIWREFDGLG
                     RNTRVGLNFTEKGFGIRAALGCSDRGHSAASQIKPGEVNWEKLFGEEGVRWFHTGGIF
                     AALAPNTSEAVIEAVEIARKYGTIVSYDLNYRASLWKSQGGKEGAQKINRNIAKYVDV
                     MIGNEEDFTACLGFEVEGLDEHISAIDPANFKKMIQTAVKQFPNFKVAATTLRNAKTA
                     TFNDWSAILYAGGSFYQSMMRENLEIFDRVGGGDGFASGLAYGFMEGKGPQAAVEYGA
                     AHGALAMTTPGDTSMVNVKEVEAVMKGKGARVIR"
     gene            complement(5105..5776)
                     /locus_tag="VEW08_02565"
     CDS             complement(5105..5776)
                     /locus_tag="VEW08_02565"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HYJ39643.1"
                     /translation="MNKDRMSVLAAMMDQGVIPVFYHPDVEVCKKVIQACANGGAKCI
                     EFTNRGDFATHVFYEVARHFDKADPSVIMGVGSIVDAPTAGIYIANGAKFVVGPILNA
                     DVAKVCNRRAIPYSPGCGSASEISYAQELGSEIVKVFPGSSVGGPEFVKNVMGPMPWT
                     RIMPTGGVEPTEESLRKWFGAGIVACGIGSNLITKELLAKQDYAGVEAKVRDTVKLIK
                     TIRGK"
     gene            6013..9375
                     /locus_tag="VEW08_02570"
     CDS             6013..9375
                     /locus_tag="VEW08_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ39644.1"
                     /translation="MANSNRTKGILPLVLGSLGVASGVSAADQDQAAAKTEGQTTSAA
                     EVAEDIGEIEVTGYRLSLRNAIDIKKNASVMKDAINAEDIADFPDANLAESLQRLPGV
                     ALDRDNGEGRGITVRGLGSDFTRVRLNGLETLSTAAAADSGTSPNRGRGFDFNVFASD
                     LFTSLEVTKTASASADEGSLGATVDLVTGKPLDYKGRKLALSMEDAYYENGGTHNPRV
                     AALFADQFFDDRFGVSLSGAYSERTSEIDRYKRQAGQSDYAYRGATFAGTLQTPRAGF
                     AAPEGASLAGTTATNGVQNPAAIAAQTGSDAAAYALLYPGAPYNTPGRFNNSLVRIPS
                     LMNIEQQDLEQERLGLTGAIQWKPTESTNIGLDMVYSKFDQKSDVNQIQSVGLNRNNT
                     NANFNALAPTVTNRRASYATCNSVTALPFREAINCGGSEVVPGGVFAGLGTTSFSTNP
                     NNLDTYDYYNNPLSPGFGASPNGMYFRDRIVGRPGVDVLAAHVSEAGNADYLELRNVD
                     WRSATDSSYFTTQFQQASLSWQQQIGDQLKMDVIYGRSRSSNDNTGLLVEFNRMDSPE
                     TFTYDERDHGSMPAINYGFDLADPNNWTLVKGFSSIRHFERETDNDYQGGHVNFDVKI
                     NDHLSLEFGILKREYQFKTNEGRRLSQEAQNPTLAELGVTTSELGRVYDFGDGLDLPA
                     GSPRAFFAPNIDKFREIIGFDCSCVNEYGDWTLGYLSNPGNQFSVTEYDTSYFMQINF
                     DYDLFGHRTFGNFGVRDADTHVKSQGYTPSPAATGPRPLEAEHTYNDKLPSLNIAYQL
                     TDTMLLRGGWSKVMARPQLANLAPTITGLTTPVIGSTTVPSVTLGNPELSPFRAENYD
                     LSWEWYFQEGGLLSVAVFKKDVSNFPQTVSTSATLQEILPPDQYQATLQTLTPQQIAW
                     VEGGGSGGGPGLYGVRQFADASGGVIEGYELSYQQDFTFLPGFWKNFGVQANYTHLTS
                     ELQYIVDPGNTLLTPTTPLRPQVTAPGPFTGASPDSANFTLYYETSKWSARASYAYRS
                     AYVSQYPIAAGTCDPGVCDAPLVNDFLGSLATRNIDAKVTWQAMEYLSFSIEALNITN
                     QTEDRWAYQDEPLVTQYSSTGRQIFAGFRLSL"
     gene            9652..12291
                     /locus_tag="VEW08_02575"
     CDS             9652..12291
                     /locus_tag="VEW08_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ39645.1"
                     /translation="MVAIILVVFYGWSQRGWLANVADIESRDVDMQSQQLQRGFATEL
                     DDLNSAMGDYAIWTETAEFARGNRPDFISQSLNAGALVRLDIDTFLVLDRNLETRASL
                     AFDATAAQEYEIPPDPELLAVIKQAVTSGQLNVNRDSVRGIVQRARGPALFAVRPVFD
                     SAGNGQPQGWIAFARAFTPTVVERIGRFSPWPVTGFPVANLDRAGVPAEVRDWVHHSP
                     LTANLLTRVAGRNHFTGYLVLRDQLGTPVWLVQLEIPRSAFAQAQRTTLYQTILLGLL
                     VGGFTIVAMLLVARSRRLNADRLSLESRYRAIIEQAQDGLLVADARTGEIVDANPAVQ
                     QQLGFTLDELRGRSVLSVLRGPNDSQDPVIATLARLEPSRGIELVQWLKDGRQMDVEV
                     SCVPIESRDRRLVSYLMRDLSERKKAQTQLLANQQRLDKLANHDTLTGLPNRLFLQAH
                     LPEAIGRCQESGQMLAVLFLDLDRFKHINDSRGHEVGDKLLQEIAKRVRAAVRPADIV
                     VRMGGDEFVVVLHKVNAPDEVAIAATRINEVLSAPVMIDGRALVATVSIGVSLFPRDG
                     ATMGELLKHSDTAMYQAKDLGRNNFQVFSPQMDRSLKERVAIESSLRIGLKVNQFDVH
                     YQPIIDIHSRRVSGLEALLRWKHPTQGYISPERFIEVAEETGLIIPIGQFVLDRIGSD
                     LARWREQGAHLVPVSMNVSAVQLERTKLRELIQQAMVKNSIGPQLIQLELTEGSLFEN
                     RTGEFREDALATLRDLGVKIAIDDFGTGYSSLSYLKRWRVDSLKIDRSFVRDIATDPS
                     DHAIVSAIVAMARSLNIQVVAEGIESWQQLEILRNMGCSLAQGFLFAKPVPAADALRY
                     LRIEPLDLVEANWEASNSLAETG"
     gene            complement(12346..14379)
                     /locus_tag="VEW08_02580"
     CDS             complement(12346..14379)
                     /locus_tag="VEW08_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ39646.1"
                     /translation="MSPSRIHRVLLVFGSLALSAMARPGDTGSTPSVTVYPASFFAAA
                     QPPSAFDMLAILPGYVFSESDSDVRGFSGAVGNVLIDGSRPANKQESLESVLRRIPAT
                     TVERIELMRAGAPGVDMQGQTALANVVRRREAQTRGSIEVESGFFERGFHSPRLAGEI
                     SRRGAQDLIEFSAAVYEVVDDEHGIGTRPRVAPDGTVLRRSDYVQDEGARIRELAGVY
                     ERTLASGRLRLSASLKSEKSGADISEHITFPEDSTSKVREFGDEGGSEFGVSFERPLA
                     GDQQLELLAIHRASGEHGGEIETESGTVTLFLQDERGSESIVRAALRWNHGQVAIESG
                     IEGALNVVDSRNTLEEDGVATPLPNGAARIEEQRGEVFAMAGWPVGAAWRLEAGSRFE
                     YSRLDLSGDSNLTKYFFFPKPRALATWSMSEHDRLRLLLEREVGQLDFEDFAGSASLS
                     SSTVTAGNPDLEPERTWRLEAAWERSFLESGALLLAARHERIDELVDRIPIIADEPFD
                     AVGNIGKGQRSELELNLTLPLDRIGIPSGLFRTVALWRDSSATDPTTNESRPISEDQP
                     REFQIHFSQHLQRARLRWGVDLNLASVSREHFFDEIRTERLGTQLDVFAQYEPTSDWY
                     VKLFANNLTDRSAVRERQIHDGIRGVAPLSFIETRTLEIGPFYGFTLRRRFGD"
     gene            complement(14451..15806)
                     /locus_tag="VEW08_02585"
     CDS             complement(14451..15806)
                     /locus_tag="VEW08_02585"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYJ39647.1"
                     /translation="MKPRSLRVRLLLSATAAIFVALAFAWVVMTLLFERHIERRVQTD
                     LVREGMQLAANLSIGPGGEPLLNREPGDVRFGEPASGLYWQVTTSAGRLRSRSLWDQA
                     LPTSLASTKHEWNMRFPDGPFEEQLLLVERQVRPDRDGPDVLIQVASENKSLHESRRE
                     FGAELAVFLCLLWAILSAAAWAQVALGLKPLARIRDQVHGLKRSSRERLAIAPAREVE
                     PLVNAINELADARENDLARARRRAANLAHALKTPLAAIAAQSRRVREAGAQDAADGLD
                     RSISAASAAVDRELARSRAAEIRAAITDVRAPALPAVESVVGVVERAEFVAQLVFQVE
                     VPSELVVPVAADDLLELMGVLIENAARYARRRVLIAGDDSAEGCRLRVEDDGPGIDPD
                     QMSSVFTRGARLDESGGGHGLGLAIARDLAEATGAEISLSRSALGGLGVAIFWRKLTL
                     A"
     gene            complement(15803..16468)
                     /locus_tag="VEW08_02590"
     CDS             complement(15803..16468)
                     /locus_tag="VEW08_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ39648.1"
                     /translation="MRALIIEDEAAVADDMARALEAAGFVVDMARDGDDAWFKGSSED
                     YDVAVLDLGLPRLDGLSVLRRWRNDSRSFPVIIVSARNDWTEKVAGIEAGADDYLGKP
                     FEIVELVARVRGLVRRAGGRVSSSIRIGSLVLDTHRMSASYAGRPIRLSQLEFRFLHY
                     VAHQSGRAVSAGELAEHLYGAEEASDTNAIEALVVRLRRKIAPEVIETRRGFGYRIAG
                     DAQ"
     gene            complement(16469..16801)
                     /locus_tag="VEW08_02595"
     CDS             complement(16469..16801)
                     /locus_tag="VEW08_02595"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HYJ39649.1"
                     /translation="MKTSNTILIATCAALFAQGIALSYADDESRTRHAREHEEMRAAL
                     QRGEVLPLARILAIAQEKVPGDVIEVELESKNGALVYEIKVLTESGRVREIKIDARKG
                     TVLEIEDD"
     gene            complement(16866..18098)
                     /locus_tag="VEW08_02600"
     CDS             complement(16866..18098)
                     /locus_tag="VEW08_02600"
                     /inference="COORDINATES: protein motif:HMM:NF018506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HYJ39650.1"
                     /translation="MSGIAAGAWLGSAEAPTKLVTMGFSPFLISLGMVMGVFVARWTI
                     PVALKGTSYVWADLREKPHLIVWGVVAGMLWAVANTLTVFAIRNVGLSVAFPLWNVNS
                     LIGMFWGWLLFNELRGSGAKQRLIVLGGATAIVIGACLLAYSTTNHDSIAQREAATLG
                     VLAALGAALLWGTMYIPYRKAYISGMNPLSFVTVFTFGELGTVFALATIFDGGVTNVL
                     DSLEQAKPVLFWLFLGGFCWVIGDLFQQYAAKYIGISRGIPLSNTNQLWGLAWGALVF
                     GELAHMADSRALIIGGSLIMIAGAVAISFAEAPADEQRHWRLAMERECARYGLDRERV
                     AAAVMGSDSSRGPRSPRHWWELLIVAAAISVFVWLGIHAERPSIHVDTSWLLVIAAAS
                     LALLAGAVAVLWKRTRFA"
     gene            complement(18270..18410)
                     /locus_tag="VEW08_02605"
     CDS             complement(18270..18410)
                     /locus_tag="VEW08_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39651.1"
                     /translation="MKPLPIKPNLIAAAIVGAPCDIADDGQASYRNGMRISITIEIGI
                     SI"
     gene            18409..19491
                     /locus_tag="VEW08_02610"
     CDS             18409..19491
                     /locus_tag="VEW08_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYJ39652.1"
                     /translation="MGRAHSIALHSVAATFGADYAVDCVVLADTTAERARDAAPSLGF
                     ARSTGNWRELIADPKIDVVDICTPNHLHAEMALAAIAAGKHVYCEKPLALDVEESAAV
                     VEAAKRAGICNAIGFNYICNPMVQAAREMIQAGEVGEVVAFSGHYVEDYMCDPRTPFT
                     WRCERRLAGAGALADLGSHLINMAHVLLGPISRVNGVVRTVHKERSETATGKVRTVEN
                     EDLANALVEFESGVPGTLHISRVAAGYKCGLTVQVVGTRGTLEFNQERMNELRLYRTD
                     DATGRRGFRTILAGPEHPDYGAFCPAPGHGLGINDLKVIEVRNLLRAIRTGTPASPDF
                     AEGLRVQRVMTAIELAAASGGWVSTR"
     gene            19488..20828
                     /locus_tag="VEW08_02615"
     CDS             19488..20828
                     /locus_tag="VEW08_02615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ39653.1"
                     /translation="MKSFLRFFATCDDKPPCHDPPTVDTLFRRHRLRIMIAITLGYGV
                     IYTCRLALGVVKKPLIDAGIFGPAELGLIGSALFYTYAIGKLTNGFLADHANMKRFLA
                     AGFALSALCNIGMGFSTAVTLSVVLWGLNGWFQSLGAPGGVVAMTAWFSNRERGRMYG
                     IWSTAHSIGEGLTFLVVGTIVAVLGWHWGFLIPAMFGLATAALAYWLVQDRPPTMGLP
                     TVAEWRNDHYVAPAVQPKSVFATQLSILKIPAIWVLALSSATIYVTRYAINSWGVLYL
                     QEARGYSLPMAGTLLMLSTIAGMVGAVAFGFISDTLFKARRPPANLLFAILELSGLLL
                     IFFGPQNTAVTIAGLVLFGMGLTGLVTSLGGLFAVDICPKRVAGAAMGLIGIFSYIGA
                     AVQENVSGHLIEQGMKMVDGVRTYDFTTVILFWIGSSFLSMLLAASLWRVRLRD"
     gene            complement(20854..>22160)
                     /locus_tag="VEW08_02620"
     CDS             complement(20854..>22160)
                     /locus_tag="VEW08_02620"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004951467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HYJ39654.1"
                     /translation="RRARVMFEFKGLLEKHRDELAAMISAEHGKVLSDAQGEVTRGIE
                     VVEFACGIPQLLKGDFTADVGTGVDSWSLRQPLGVCVGITPFNFPAMVPLWMIPIAIA
                     AGNTFVLKPSEKDPSCPLRIAELLLEAGAPNGVLNVINGDKIAVDALLAHPHVKAVSF
                     VGSTPVARAVQRGAIEHGKRVQALGGAKNHAVVMPDADLDFAADALMGAAYGSAGERC
                     MAISVAVAVGDSADGLVAALSRRLQKLRIRESRDRDADMGPLVTREHRDKVRSYVDLG
                     CQEGAKLVADGRGFSAPGFENGFFLGASLFDQVNPRMRIYREEIFGPVLCVVRVPTLA
                     AALELVNSHEFGNGAAIFTNDGRAAQEFSRGVEAGMVGVNIPIPVPMAFHSFGGWKQS
                     MFGDMHMHGPEGVRFYTRQKTVTSRWPKSGTSVAEFVMPTMK"
BASE COUNT         4174 a         7254 c         6700 g         4032 t
ORIGIN      
        1 gcaggcaccg agatcgacga agtcatcgtc accggtttcc gcggatcgct gcagaccgcc
       61 gccgccgaga agcgcgccaa cgtcaatttc acggattcga tcttcgccga ggacatcggc
      121 aagttccccg acctcaatct cgccgaatca ctgcagcgca ttcccggcgt gcagatcgcg
      181 cgcgacttca ccggtgaagg cacgtcggtc gcggtgcgcg gtctcggtcg cgatttcacg
      241 cagatcacgc tgaacggcgc gccggtagaa accgcgtccg acagcaacat cgacagcgtc
      301 agccaggggc gcggaatcga cctcgtgtta ttcccgacgg aactgttccg tcagctgacg
      361 gtgagcaaga ccccgaccgc caagcagcag gaaggcgcgg ttgccgcgaa catcgacctg
      421 cgcgtcgcaa ggccgttcga cgataaaggg tttcacgtca actacagcgc gaagggcagc
      481 ttccaggaat cgagcgaaga gtggagcccg cgcctggggt tctacgcgag caacacctgg
      541 gacaccggca tgggcgaatt cggcatcctc ggcggcgtcg cctattcgaa tcgcagttac
      601 cgttccgacg gattcaacac catcggtttc acgaccgtct cgctcggcaa ccgctgcctg
      661 tacgccaccg atccgcagtg caacagcttc gtcacgccta acaacgacac gggttacggc
      721 aaccaggcac aggcctggcc gaccacggtt cctgccggtt acagcttcgg cgcaaatggc
      781 gcgaacgcgg gcgacccgct gaccgtatgc ggcgccggcg acaccgctgg cggcacctcg
      841 aacctgtcct gccgcgacct gagcttcgcc atctggccgc gtctcgcccg cccggacatg
      901 ctcatcggcg agcgcgaaac cacttccgga atcatcacga tgcagtgggc gccctcggac
      961 aagctcgagt tctacatcga cagcctgatc tcgaaggccg accatggtta cgagcgcaac
     1021 gacctcaacc tcgcagtccg cagcacgaat accaacgttc ccgtgaacgt ggtgctcaac
     1081 gaagacaaca tcgtgacgag cgcgaccatc gcgaatccgg tctggctcaa cgaaaaccgg
     1141 ccgtatcacg aagacacgga tttcctcaac ctcaacggcg gcatgaagtg gcagatcggt
     1201 gagaagttca ccgtcgacgc gtcgatcaac tacaaccaca gtgactggtt ccgctcgacc
     1261 aatacctatc tgttcaattc gaacctcaat accggcatca cggtcgatct cgaagccaat
     1321 ggtgacggcg tgatcaaggt gacgccgagc cgcgatctga acgacctcac cttctggaac
     1381 tggaatgcgc tgcgcgttca gccggtcaag cgcgagcttt ggcagaaagg cgcaagagtg
     1441 ggcggcgaat gggcgttcgc cgatgcgttc aaactcggcg cgggcttctc acgagacacg
     1501 ttccatcgcg agatcaccaa ctgggaaacg acgagctgcg caaccgacgg tggaaatacc
     1561 acgagtcctt ccggccgcga gtgcgcggcc gcgctggcgg ctgcgggcat ccagcacgct
     1621 aaagttgcga ttcccaatgc ccagctgcgc aattacatgc aagcctggac acacggtgcg
     1681 ctgtatcaga cgtcggattt cgacgtcggc gtgaacaacg gctgggcgct gcctaactac
     1741 agcctgctcg acccggcaac caacctcgat ttcttcgaga aggaactcgc tgtcggcctg
     1801 gtcggcggtc tcaacgtcgc cggctacaac ccgcgcgtgc tcgacgagga tacggatgcg
     1861 ttctacctcg aagcaaacgg tcagctcgag atgctcggca acctgcgtta caccctgggt
     1921 gcacgctaca tcaagacgga tcagttcgtc tccggctaca tcagcgcgaa tactcctgac
     1981 ggtgcggtgc gcacgtatgt cgaagacgcc gccgactatg acaaggtgct gccgagcctg
     2041 aacgtggcga gcgagctcgg tcacggcctg gtgttgcgcg tcgcggcgtc ggaaacgatg
     2101 acacgtgcgc agcccggcga catcgctccc aaccagtcgc tgagcatcaa cggcgacgtg
     2161 ttgacgattg gcaacgcggc gctcacaccg tacttctccc aaaacatcga cctggggctc
     2221 gagtggtact tcggtgattc cgggctcggc atgattgccg tgaacgcctg gaccaaggag
     2281 atcgagggtt acaccacgat cgtcggaacg gtgactccgt tcggccagtt gggcatcgat
     2341 tactcgacgc tgccgattgc cacgcagacc ggtattcaga acactgccaa tgcgcagtgc
     2401 ggctgcacca acggcaatcc ggccaacgca aacgttcgtg tcgaccagcg tcagaacacc
     2461 agcgagctga tcacgctcga tggctgggaa ctgacctacg tacagccgct cgacttcctg
     2521 ctgcagggcg ccgggttcac ggtgaactac acgcacatcg atcaggagtc ggagggcggc
     2581 ctgccgggcg ctccatcgag cgcgatcacg ggtctctcgc ccttcaccta caacatcgcg
     2641 gccttctacg agaactatgg attctcgggt cgtctgacgt attcggtgcg tgacgccttc
     2701 gtcgaattcc tcggcaacaa cgaaaacaac attgccggcg acaactacgc gcaggatcgc
     2761 aaatacctcg atgcgtcgtt cagctacaag ttgccgatgg acacggacat ttccatcagc
     2821 ctcgaagtgc agaacatcct caacgaacag ctgctgacgt acttccgcag cgacgagttc
     2881 accccgcgcg cttcgttcgc gcccggccgc cagatgctgc tgggcataag cggttcgttc
     2941 tgatttgaaa tagtctgcgt gctcgaacga ggcaggggag gccgcggaag ctgcctcccc
     3001 tttttcttgt cagcagcccg cgttcagcgg gattgggcca gcatccagga gtacagatcc
     3061 tggccttcgt agatccgcac ccaggccaga tgacccagct cttccgcgac ggtgaagcga
     3121 acctcgggat gaccgagctc ctcgagcttg ttcagggccg gatagaaaaa acgcagcggc
     3181 accaccggat cgcgtccgcc cgcaaacacc cacaaaggta gtttggcgcg cgcgatcgga
     3241 cccgcgtgat ccgggtggcc gtagccgacg atgggcgcga gagcggcgaa cttctccgga
     3301 tgacgtgccg cgagaaacca tgcgccaaaa ccgccgtaac tcaggcccgt caggtagacg
     3361 cgtttcgcat cacccttgaa gtccgcgatc gtgcgatcga ccatcgccag caactcgttc
     3421 tcgatctcgt tccagccatc gggcggaccc tcggggccat ccggcagctt gtcgtccgag
     3481 agttttccct gcatggcttc cgccaatcgc aagactcgac cgtcgggccg cggattggtc
     3541 cagttcgcaa gccgctgcgg tatctggtcc cgcgtgcgat tcctgatgta atccacgtcc
     3601 ccctggccaa acatcggcag ctgcggcgag atgatcacga aaggcaggtt ccgtctctga
     3661 caccacgcct cgaacatggg accgtgcacg agcacgtagt cgagatcctt cctggcgtcg
     3721 ccgcgttcgc cgttaccgtg cagaaacaac atgaccggcc actgcttcga ctgcgcgaat
     3781 ccgcgtggaa gatagacgaa gtagtcgcgc tgtttgccgg tgcgctcgct ctgataggaa
     3841 atgcgcttga gctcgggctc tgacagggcg gtgtgggctg gcgcggccgc cgccgccaat
     3901 tgcgaatcgg aagcggccga agcaccggcc gctcccgcca ggagggggaa cacttctctg
     3961 cgtgtggtca tggatccccc tttttgccgc ttcatcgaat gacgcgcgcg cctttgccct
     4021 tcatcacggc ctcgacttcc ttgacgttga ccatcgaggt atcgccgggc gtggtcatcg
     4081 ccagcgcgcc gtgcgcggcg ccgtattcca ccgccgcctg cggacctttg ccttccatga
     4141 agccgtacgc caggccggag gcgaagccat cgccgccgcc aacgcggtca aagatctcga
     4201 ggttctcgcg catcatcgac tggtagaaac tgccgccggc gtaaaggatt gccgaccagt
     4261 cattgaacgt cgcggttttc gcatttcgca gcgtggtcgc ggccaccttg aaattcggga
     4321 actgcttcac cgcggtctgg atcattttct tgaagttggc cgggtcgatg gccgagatgt
     4381 gctcgtcgag tccttcgact tcgaatccga ggcatgccgt gaagtcttct tcattgccga
     4441 tcatcacgtc gacgtacttc gcgatgttgc gattgatctt ctgcgcgcct tccttgccgc
     4501 cctggctctt ccagaggctg gcgcggtagt tgaggtcgta cgaaacgatg gtgccgtact
     4561 tgcgcgcaat ctccacggcc tcgatgaccg cttccgacgt gttgggcgcg agcgccgcga
     4621 aaatgccgcc ggtatggaac cagcgcacgc cttcctcgcc gaacaacttt tcccagttca
     4681 cttcgccggg tttgatctgc gatgccgccg agtggccgcg atcagagcat ccgagcgccg
     4741 cgcgaatgcc gaagcctttc tcggtgaaat tgaggccgac gcgcgtattg cggccgaggc
     4801 catcgaactc gcgccagatg atcttcgagg tgtcgacgcc gccctgcatc atgaggtctt
     4861 cgaccagcca tcccaggtcg ttctgcggca gcgctgtcac ggcagtcgag cgtttgcccc
     4921 agcatttgcg catggcccgc gcgacgttgt attcgccgcc gccttcccac acctggaacg
     4981 tacgcgcgtt gcgaacgcgg ccgaaaccgg gatcgaaacg cagcatgatc tcgccgaatg
     5041 aggcgcagtc ccatcgggtc tctgctgcgg atctgatttt cagaacgttg tcggtcatgt
     5101 ctgtctactt gcctcgaatg gtcttgatga gtttcacggt gtcgcgcacc ttcgcttcga
     5161 cgccggcgta gtcctgcttc gcgagcagtt ccttggtgat gagattgctg ccgatgccgc
     5221 acgcgacgat gccggcgcca aaccacttgc gcaaggattc ttcggtgggc tccacgccgc
     5281 cagtcggcat gatgcgcgtc cacggcatcg ggcccatgac gttcttcacg aactctggtc
     5341 cgccgacgga cgagcctggg aagactttga cgatttcgct gccgagttcc tgcgcgtacg
     5401 aaatctcact cgcggagccg cagcccgggc tgtagggaat cgcacgacga ttgcagacct
     5461 tggcgacgtc ggcgttgagg atcgggccga cgacgaactt cgcgccgttg gcgatgtaga
     5521 tgccggcggt gggtgcgtca acgatagagc cgacgcccat gatgacgctc gggtccgcct
     5581 tgtcgaagtg gcgtgcgact tcgtagaaca cgtgcgtggc gaaatcacca cgattggtga
     5641 attcgatgca ctttgcgccg ccgttcgcgc aggcctggat caccttctta cagacctcga
     5701 catccgggtg atagaaaacc gggatgactc cctggtccat catggcggct agcacgctca
     5761 tacgatcctt attcatccat ctctccgttc atttgtgagg cgccggcatt ttcgttgacg
     5821 ggtgacggcg cccgcaaatc aatcgtgaag actcgcaatg ctcgtgtccc gaaacaagct
     5881 ccatcgtttt ccatatcccg ctcacgcttg cgctcgcgtt tggagtcgtc tataacgacc
     5941 cccaaggtaa ccggtgtcat ttttgcgccg gacgtacctc ataaacaaca agtatcgaat
     6001 gggaggggga cgatggcgaa ctcaaatcgc accaagggta ttctgccgct ggtgcttgga
     6061 tccttaggcg ttgcaagcgg tgtttcggcc gctgaccagg atcaggccgc agccaagacc
     6121 gaaggccaga ccaccagcgc agcggaagtt gccgaagaca ttggcgagat cgaggtaacc
     6181 ggttaccgcc tgtcgctgcg caatgccatc gacatcaaga aaaacgccag cgtcatgaag
     6241 gacgccatca acgccgagga catcgccgac tttcccgacg ccaacctcgc ggagtccctg
     6301 cagcgcctgc ccggcgtcgc gctcgaccgt gacaacggcg aaggtcgcgg catcacggtg
     6361 cgcggcctcg gcagcgattt cacgcgcgtg cgcctcaacg gacttgagac gctttccacc
     6421 gcggctgccg ccgattcggg cacatcgccc aatcgcggcc gtggcttcga tttcaacgtt
     6481 tttgcgtcgg atctcttcac gtcgctggag gtcaccaaga cagcctcggc atccgcggat
     6541 gaaggctcgc tgggcgccac cgtcgacctc gtgaccggca agccgctcga ctacaagggc
     6601 cgcaagctcg cgctctcgat ggaagatgcg tattacgaaa atggcggtac gcacaacccg
     6661 cgagttgctg cgttgttcgc ggatcagttc ttcgacgacc gattcggcgt gtctctgtcg
     6721 ggtgcataca gcgaacgtac ttccgagatc gaccgctaca agcgccaggc cggccagtcc
     6781 gactacgcgt accgcggtgc gacctttgcc ggcacgctgc aaacgccgcg cgccggtttc
     6841 gccgcgcctg agggcgcatc tttggccggc accacggcaa caaacggtgt ccagaatccc
     6901 gcggcgatcg ccgcgcagac cgggtcggac gccgccgcgt acgcgttgct gtacccgggt
     6961 gctccgtaca acactccggg ccgtttcaac aattcgctgg tgcgcatccc ttcgctgatg
     7021 aacatcgagc agcaggacct cgagcaggaa cgcctgggtc tgaccggcgc aatccagtgg
     7081 aagccgaccg agagcaccaa catcggcctc gacatggtgt attcgaagtt cgaccagaag
     7141 agcgacgtga accagattca atccgtcggt ctgaatcgca acaacacgaa tgccaatttc
     7201 aatgccctgg ctcccaccgt gacgaatcgc cgcgcgtcat atgcgacctg caacagcgtg
     7261 accgcactgc cgttccgcga agcgatcaat tgcggcggct cagaggttgt gccgggcggt
     7321 gtcttcgcgg gcctgggcac gacgagcttc agcaccaacc ccaacaatct cgatacctac
     7381 gactactaca acaacccgtt gtcgccgggc ttcggcgctt cgcccaacgg tatgtacttc
     7441 cgtgaccgga tcgtcggccg gccgggcgtg gatgtgctcg ccgcccatgt ctccgaggca
     7501 ggaaatgccg actatctaga gctgcgcaac gtggactggc gctcggcgac cgactcgtcc
     7561 tatttcacga cgcaattcca gcaggcttca ttgagctggc agcagcaaat tggcgatcag
     7621 ctgaaaatgg acgtgatcta cggccgttcg cgttcgagca acgacaacac cggtctgctg
     7681 gtcgaattca accgcatgga ttcgcccgag accttcacct acgacgaacg cgatcacggc
     7741 tccatgccgg cgatcaacta cggattcgac ctcgccgacc ccaacaactg gaccctggtg
     7801 aaaggcttct cgtcgatccg tcatttcgag cgtgagaccg acaacgacta ccagggtggg
     7861 cacgtcaatt tcgacgtcaa gatcaacgac cacctgtccc tggaattcgg catcctgaag
     7921 cgcgaatacc agttcaagac caacgaggga cgacggcttt cgcaggaagc gcagaatcct
     7981 accctggccg agctcggcgt caccacttcg gaactgggtc gcgtctacga tttcggcgat
     8041 ggcctcgacc tgccggcggg ttcgccgcgc gcgttttttg ccccgaacat cgacaaattc
     8101 cgcgaaatca tcggcttcga ctgcagctgc gtgaacgagt acggcgactg gaccctgggg
     8161 tatctctcga accccggcaa ccagttctcc gtcaccgaat acgacaccag ctatttcatg
     8221 cagatcaatt tcgattacga cctctttggc caccgtacct ttggcaactt tggtgttcgc
     8281 gacgccgaca cacatgtgaa gtcgcaaggc tacacgccga gccctgctgc gacgggcccg
     8341 cggccgctcg aggccgagca cacctacaac gacaaactgc cgtccttgaa catcgcctat
     8401 caactcaccg acaccatgct gttgcgtggc ggctggtcga aggtgatggc gcgtccgcag
     8461 ctggcgaacc tggctcccac catcacgggt ctcacgacgc cggtcattgg gtcgaccacg
     8521 gtgccttcgg tgacgctcgg caatccggaa ctgtcgccgt tccgcgcgga aaactacgac
     8581 ctgtcatggg agtggtactt ccaggaaggc ggtctcttgt cggtcgcggt gttcaagaag
     8641 gacgtgtcga acttcccgca gaccgtttcg acgtccgcaa cgctgcagga aatcctcccg
     8701 cctgatcagt accaggcaac gcttcagacc ctgacaccgc agcagatcgc ctgggtggaa
     8761 ggcggcggca gtggcggcgg gcccggcctc tatggcgtac gtcagttcgc cgacgcgtcg
     8821 ggcggcgtaa tcgagggtta cgagctcagc tatcaacagg acttcacgtt cctcccgggg
     8881 ttctggaaaa acttcggtgt tcaggctaac tacacgcacc tgacctcgga actgcagtac
     8941 atcgtcgatc cgggcaatac cctgttgacg ccgaccacgc cgttgcgtcc gcaggtcacg
     9001 gccccgggac cgttcaccgg agcatcaccg gattcggcca acttcacgct gtactacgag
     9061 acgtccaaat ggagcgctcg cgcgtcctat gcgtaccgtt cggcgtacgt gagccagtat
     9121 ccgattgccg ccggcacttg cgatcctggc gtctgcgatg ctccgctcgt caacgacttc
     9181 ctcggcagcc tcgccacgcg caacatcgat gcgaaggtga cctggcaggc catggaatac
     9241 ctgtcgttct cgatcgaggc tctgaacatc acgaatcaga ccgaagatcg ctgggcgtat
     9301 caggacgaac cgctggtcac gcagtactcg agcacgggac ggcagatctt tgccgggttc
     9361 cggctcagcc tgtaatccga ccgggcagtt gctaccgcac acgaagcgcc ggcgcctgaa
     9421 tcaggcgccg gcgcttcgtc gtttccgggc ctggccttgt gccccttact cgctctgacg
     9481 ccccattatt aaggggaagt gtgcgcggtt cgatgccggc ctgagggggc cgccgggcaa
     9541 actcaagagt ggccaagaaa tgcacgtgac gcagttcacg ttatgacccg taccagcaaa
     9601 ccggaaccga aaacgaggat ccgaaagtca ccgctgcccg taattggcgc ggtggtcgcg
     9661 atcattctcg tcgtgttcta tggctggtcg cagcgcggct ggctggccaa cgtcgccgac
     9721 atcgagtcgc gcgatgtcga catgcagagc cagcagctcc agcgcggttt cgcgaccgag
     9781 ctcgatgacc tgaattccgc catgggcgac tacgcgatct ggaccgagac cgcggaattc
     9841 gcccgcggca atcgtccgga cttcatttcc cagtcgctca acgccggcgc gctggtgcga
     9901 ctggacatag acaccttcct ggtgctcgac cgcaacctgg aaacccgcgc ttcgctggcg
     9961 ttcgatgcga cggccgcgca ggaatacgag atcccgcctg accccgagtt gctggcggtg
    10021 atcaagcagg cggtgacgtc cggtcagctg aacgtcaatc gcgacagcgt gcgaggcatc
    10081 gtgcagcgcg cacggggacc ggcgctcttc gccgtgcgcc cggtattcga cagcgcgggc
    10141 aatgggcaac cgcaaggctg gattgcattc gcacgcgcgt ttacacccac ggtggtggag
    10201 cgcatcggcc gcttctcgcc ttggccggtt accggctttc ccgtggccaa cctggatcgc
    10261 gccggcgtgc ctgcggaggt gcgcgactgg gtccaccatt cgccgctgac ggcgaacctg
    10321 ctgactcgcg tggcgggacg taaccatttc accggctacc tggtgctgcg cgatcagctc
    10381 ggcacgccag tctggctggt gcagctcgag attccgcgca gcgcgttcgc acaggctcag
    10441 cgcaccacgc tctaccagac catcctgcta gggctgctgg tcggcggatt caccatcgtc
    10501 gcgatgctgc tcgtggcgcg ttcgcgccgg ctcaatgcgg accggctgtc gctcgagtcg
    10561 cgttaccgcg ccatcatcga acaggcgcag gacggattgc tggtggccga cgcgcgcacc
    10621 ggcgagatcg tcgatgccaa cccggccgtt caacaacaac tgggcttcac gctggacgag
    10681 ctgcgcggcc gttcggtcct gtccgtgctg cgcggcccga atgattcgca ggatccggtc
    10741 atcgcgacgc tggcgcgcct ggagccgagc cgcggtatcg aactggtgca gtggctcaag
    10801 gacggccggc aaatggacgt cgaagtgagc tgcgtgccca tcgagtcgcg cgaccgccgc
    10861 ctcgtgtcat acctcatgcg cgacctgtcc gagcggaaga aggcgcagac gcagttgctc
    10921 gccaaccagc agcgcctcga caaactcgcc aatcacgaca cactcacggg cctgccgaat
    10981 cggctgttcc tgcaggccca tctgccggaa gccatcgggc gctgccagga gtcggggcag
    11041 atgctcgcgg tactgttcct cgatctcgat cgcttcaagc acatcaacga ttctcgcggc
    11101 catgaagtgg gcgacaagtt gctgcaggaa atcgccaagc gtgtgcgggc ggccgtgcgc
    11161 cccgcggaca tcgtcgtgcg aatgggcggc gatgaattcg tcgtggttct tcacaaggtg
    11221 aatgcacccg acgaagtcgc cattgccgcc actcgcatca acgaagtgct ctcggcgccg
    11281 gtgatgatcg acggccgggc gctcgttgcc acggtgagca ttggcgtcag cctgttcccg
    11341 cgagatggcg ccaccatggg cgaactgctc aagcattcgg acaccgcgat gtaccaggcc
    11401 aaggatctgg gcaggaacaa tttccaggtg ttcagtccgc agatggaccg cagcctcaag
    11461 gaacgcgtgg ccatcgaatc cagcctgcgc atcggactga aggtgaatca gttcgacgtg
    11521 cattatcagc cgatcatcga catccattca cggcgcgtct ccggcctcga agcgctgttg
    11581 cgctggaagc atcccacgca gggatacatc tcgccggagc gcttcatcga agtcgccgaa
    11641 gaaaccggcc tcatcattcc catcgggcag ttcgtgctcg atcgcatcgg cagcgatctc
    11701 gccaggtggc gcgagcaggg cgcgcacctc gtgccggtca gcatgaacgt gtccgcggtg
    11761 cagctggaac gtacgaaact tcgcgagctc atccagcagg cgatggtcaa gaacagcatc
    11821 ggtcctcagc tcatacagct cgagctcacc gagggctcgt tgttcgagaa ccgaaccggt
    11881 gagtttcgcg aggatgccct cgcgacattg cgcgacctgg gcgtgaaaat cgccatcgac
    11941 gatttcggga cgggttattc gagcctttcg tacctcaagc gctggcgcgt cgactcactc
    12001 aagatcgacc gcagcttcgt gcgcgacatc gccaccgatc catccgatca cgccatcgtc
    12061 agcgccatag ttgcgatggc gagaagcctc aatatccagg tcgtcgccga aggcatcgag
    12121 tcatggcaac agctcgagat cctgcgcaac atgggttgca gcctcgcgca aggattcctg
    12181 ttcgccaaac ccgtgcctgc tgccgatgcg ctgcgttacc tgcgcattga gccgctcgac
    12241 ctcgtcgagg ccaactggga agcctccaac tccctcgccg agaccggtta gctgatcgct
    12301 gccgcgaggc ccgtcctttc gggttgcgat cgcaacgcgt tacggtcagt cgccgaatct
    12361 ccgtcgcaac gtgaacccgt agaaggggcc gatctccagg gtacgcgtct ctatgaagct
    12421 cagcggcgcc acgccgcgta tcccgtcatg gatctggcgc tcgcgcacgg cactgcggtc
    12481 ggtcaggttg tttgcaaaca gtttcacgta ccagtcggac gtgggctcgt attgggcgaa
    12541 cacgtccagt tgcgtcccga gccgctcggt gcggatctca tcgaaaaagt gttcccgcga
    12601 aacgctcgcg aggttcaggt ccacacccca tcgcaaccgc gcgcgctgca gatgctggct
    12661 gaaatgaatc tggaattctc gcggctggtc ttcggagatc ggccgcgact cattcgtggt
    12721 gggatctgtc gcggagctgt cacgccataa ggcgacggtc cggaacaaac ccgacgggat
    12781 gccgattcga tcgagcggca gcgtcaggtt gagctccaac tccgagcgtt gtcccttccc
    12841 gatgttgcca acggcatcaa acggttcgtc ggcgatgatg ggaattcgat cgacaagttc
    12901 gtcgatgcgc tcgtggcgcg cggcgagcag cagcgcgccg gattccagga agctgcgctc
    12961 ccaggcagcc tcgagtcgcc aggtgcgctc gggctccagg tccgggttgc ccgccgtcac
    13021 ggtgctcgac gacaacgacg ccgatcctgc gaagtcctcg aaatccagtt ggcccacctc
    13081 acgctccaga agcagtcgca gccggtcatg ttcggacatc gaccatgtcg ccagcgcacg
    13141 gggctttgga aaaaagaaat atttggtcag gttgctgtcg ccgctcaggt cgagccggga
    13201 gtattcgaat ctcgagcccg cttccagccg ccacgccgcg ccgactggcc aacctgccat
    13261 cgcaaaaacc tcgccacgct gttcttcaat gcgcgccgcg ccattcggaa gtggcgtggc
    13321 gacaccatcc tcctcgaggg tgttgcgact atccaccacg ttcagcgcgc cctcgatgcc
    13381 gctttcaatc gcgacttgcc cgtgattcca ccgcagcgcg gcgcgaacga tcgattccga
    13441 accgcgctcg tcctgcagga agagtgtgac ggtgcccgat tcggtttcga tttcgccgcc
    13501 gtgctcgccg gaggcgcgat gaatggccaa caactccagt tgctgatcgc cggccagcgg
    13561 ccgctcgaag ctcacaccga attccgatcc accctcgtcg ccaaattcgc gcaccttgct
    13621 cgtgctgtcc tcgggaaagg tgatgtgctc cgagatatcc gcgccggatt tttcggactt
    13681 gagtgacgca ctcaggcgca gccgcccgct cgccaatgtg cgttcgtaga cacccgccag
    13741 ttcccggatc cttgcaccct cgtcctggac gtagtccgat cgacgcagca ccgtgccgtc
    13801 gggagccacg cgcgggcgag tgccgatgcc atgctcgtcg tcgacgactt cgtagacggc
    13861 tgcggagaac tcgatcaagt cttgcgcccc gcggcgggag atttctcccg ccaggcgtgg
    13921 tgagtggaag cctcgttcga aaaagcccga ttccacctcg atcgagccgc gcgtctgcgc
    13981 ttcgcgccgg cgcacgacgt ttgcgagcgc tgtctgaccc tgcatgtcga cgcccggtgc
    14041 tccggcacgc atcagctcga ttcgctcgac ggtggttgct ggaattcgac gcagcaccga
    14101 ttcaagcgac tcctgcttgt tcgcgggccg actgccgtcg atgagcacat tgccgaccgc
    14161 gccggaaaat ccgcggacgt ccgaatcact ttcgctgaag acgtagccag gcaggatagc
    14221 gagcatgtcg aacgccgacg ggggttgcgc tgcggcgaag aacgaggcgg ggtaaaccgt
    14281 gacggaaggt gtcgaacccg tgtcacctgg ccgcgccatc gccgacagtg caagcgagcc
    14341 gaacactaac aacacacggt ggatgcggga gggcgacatg atgacctgac ggtagagtcg
    14401 atgcgcacca tcctgggctc acgcacctga cacgaggatc gctgcactcg tcaggcgagc
    14461 gtcagcttgc gccagaagat cgcgacgccg agcccgccga gtgcggaccg cgacagtgaa
    14521 atctccgcgc cagtcgcctc cgccagatcc cgcgcaatgg cgaggccgag gccgtgaccg
    14581 ccgccggatt cgtccagccg tgcaccccgc gtgaacacgg agctcatctg gtccggatcg
    14641 attccgggac catcatcctc gacgcgcagc ctgcagcctt ccgcggagtc gtcgccggcg
    14701 atgagcacac gccggcgtgc ataacgcgcg gcgttctcga tcagcacccc catcagctcg
    14761 agcaggtcgt ccgcggccac cggcaccacc agttctgacg gcacctcgac ctggaacacc
    14821 agctgcgcga cgaactccgc gcgctcgacg acgccgacca cggattcgac agcgggcagg
    14881 gctggcgcac gtacatcggt gatcgcggca cgaatctcag cggcacggct gcgcgcgagc
    14941 tcccggtcca ccgcagcgct cgcggcgctg atcgaacgat cgagtccatc ggccgcatcc
    15001 tgtgccccgg cttcgcggac ccggcggctt tgcgcggcga tggcggcgag cggcgtcttc
    15061 agcgcgtggg cgaggttggc ggctcggcgc cgcgcacggg caagatcgtt ttcacgcgca
    15121 tcggcgagct cgttgatcgc attcaccagc ggctcgactt cccgcgccgg ggcgatcgcc
    15181 aggcgttcgc gtgagctgcg ttttagaccg tgcacctggt cgcggattcg cgcgagtggt
    15241 ttgaggccca gcgccacctg cgcccacgcc gcggcggaga gaatggccca cagcaggcag
    15301 aggaacaccg cgagttcggc gccgaactca cggcgcgatt catgaagcga tttgttttcg
    15361 gaggcgacct ggatcaggac gtcggggccg tcgcgatcgg gcctgacctg tcgctcgacg
    15421 aggagaagct gttcctcgaa aggaccgtcg gggaaccgca tattccattc gtgcttcgtg
    15481 gatgccagcg aggtgggcag ggcctgatcc cagagcgagc gtgagcgcag acggccggcg
    15541 gacgtcgtca cctgccagta caagccgctg gcgggctcgc cgaagcgaac gtctccgggt
    15601 tcgcggttca acagcggctc gcctcccggt cctatcgaca gattcgcggc gagctgcatg
    15661 ccctcgcgca cgagatccgt ctgcacgcga cgctcgatgt ggcgctcgaa cagcaatgtc
    15721 atgacgaccc aggcaaatgc gagcgcgacg aaaatcgccg cggtcgcgct caacagcaac
    15781 cgcacgcgca acgatcgcgg tttcattgcg catcacctgc gatgcgatag ccaaatccgc
    15841 ggcgcgtctc gatcacttcc ggtgcgatct tgcgtcgcag tcgcacgacc agcgcctcga
    15901 tggcattcgt gtcgctcgct tcttcggcgc cgtacagatg ctcagccagc tcgccggccg
    15961 acaccgcgcg gccgctctgg tgcgccacgt agtggagaaa acggaactcg agctgtgaca
    16021 gccgaatggg ccggcctgcg tagctcgcgg acatgcgatg cgtatcgagc acgagactgc
    16081 cgatgcggat cgacgaagac acgcgtcctc cggctctccg tacgagcccg cgcacccggg
    16141 cgacgagttc gacgatctcg aacggcttgc ccaggtagtc gtcggcgccg gcctcgatgc
    16201 ccgcgacttt ttccgtccag tcgttgcgcg cggagacgat gatcacggga aacgatcgac
    16261 tgtcgttacg ccagcggcgc aacaccgaga ggccgtcgag gcgtggcagt ccaaggtcga
    16321 ggactgccac gtcatagtcc tcggaggacc ctttgaacca tgcatcgtcg ccatcgcgtg
    16381 ccatgtcgac gacgaaaccg gcggcctcga gtgcgcgggc catatcgtcc gccaccgcgg
    16441 cttcgtcttc gatgatcagc gcgcgcaatc agtcgtcctc gatctcgagg accgtcccct
    16501 tgcgcgcatc gatcttgatc tcgcgcacac gtccgctctc cgtcagaacc ttgatctcgt
    16561 agacgagtgc gccgttcttg ctctccagct ccacctcgat gacatcgccc gggacttttt
    16621 cctgcgcgat cgccagtatc ctggcaagcg gcagcacttc accgcgctgg agcgcagcgc
    16681 gcatctcctc gtgttcgcgc gcatggcgcg ttcgactttc gtcatcggcg taactcagcg
    16741 ctatgccctg ggcgaacagt gccgcgcacg tggcgatcag tatcgtgttg ctggttttca
    16801 tggcgccatg atgccggtgc aaagctgact tccgcctgac gacccgtaac ttgcggcgcg
    16861 cgccctcagg cgaagcgcgt gcgcttccag aggaccgcca cggcgcccgc caacagggcc
    16921 aacgatgccg ccgcgatgac cagcagccag ctcgtatcga catggatgct cggccgctcg
    16981 gcatgaattc caagccagac gaaaaccgaa atcgcggcgg ccactatcag caactcccac
    17041 caatggcgtg gggaacgagg ccctctcgaa gaatcggacc ccatgacggc ggcggcgacg
    17101 cgctcacgat ccaggccata acgcgcgcat tcccgctcca tggccagccg ccagtgacgc
    17161 tgctcgtccg cgggcgcttc ggcgaaactg atggccaccg ctccggcaat catgatcagc
    17221 gagccgccga tgatcagcgc acgcgagtcg gccatgtgcg ccagctctcc gaacaccaac
    17281 gcgccccacg cgagacccca gagctgattt gtgttcgaga gcggaatgcc gcgactgatg
    17341 ccgatgtatt tggcggcgta ctgttggaac aggtcgccga tcacccagca aaatccgcca
    17401 aggaacaacc agaacagcac gggcttcgcc tgctccagtg aatccagcac attcgtgacg
    17461 ccgccatcga agatcgtcgc cagcgcgaac accgtgccca gctcaccgaa agtgaacacg
    17521 gtgacaaaag acagtggatt cataccgctg atgtaggcct tgcgatacgg tatgtacatc
    17581 gtgccccaga gcagggctgc gccaagcgcc gcgagaacgc cgagggttgc ggcctcacgc
    17641 tgcgcgatcg aatcatgatt ggtggtcgaa tatgcgagca ggcacgctcc gatgacgatg
    17701 gctgtggcgc cgcccagcac gatcagccgc tgttttgcgc cgcttccacg cagctcattg
    17761 aagagcagcc aaccccagaa catgccgatg aggctgttga cgttccacaa cggaaaagcg
    17821 accgacagcc cgacgttccg aattgcgaat accgtcagcg tgttcgcgac cgcccacagc
    17881 attcctgcaa cgactcccca aactatcaag tgcggcttct cccgcagatc cgcccagacg
    17941 taagaggtgc ccttgagcgc gaccggaatc gtccatcgcg ccacgaacac gcccatgacc
    18001 atgccgagcg agatgagaaa aggagagaag cccatcgtca ccagtttggt cggagcttct
    18061 gccgagccga gccaggcgcc ggccgcgatg ccagacacga ctcccaccgc gtgcaacgac
    18121 attatggtcc tgggtttttc cgacgcgctc atgcgagcgc cgcaagcagg ctgatgccaa
    18181 gcaccagcac gaccagggga atccacaagt gaggatcggt cagcgtggct ttcaggcgtg
    18241 actgtttcat cgggttcccc gcgtgtcggt taaatcgaaa ttccaatttc aatagtaatt
    18301 gaaattcgca ttccattgcg atagcttgct tgcccgtcgt cagcgatgtc acacggcgcg
    18361 cccacaatgg cggcagcaat cagatttggc ttgatcggca gcggtttcat ggggcgcgca
    18421 cattccatcg cgctgcattc cgtcgcggcg acattcggcg cggactacgc cgtcgattgc
    18481 gtggtgctcg cggacaccac cgccgaacgc gcccgggacg cggctccctc actgggtttc
    18541 gcccgctcca ctggcaactg gcgcgagctc attgcggatc cgaagatcga cgtcgtcgac
    18601 atctgcacac caaatcacct gcacgcggaa atggcgctgg ccgcaatcgc ggcgggcaaa
    18661 cacgtgtact gcgaaaagcc gctggcgctg gacgtggaag agtcggcggc ggtcgtcgaa
    18721 gcggcgaagc gcgcgggcat ctgcaatgcc atcggcttca actacatctg caacccgatg
    18781 gtgcaggcgg cacgcgagat gatccaggcc ggagaggtgg gcgaggtggt cgcattcagc
    18841 gggcactacg tcgaagacta catgtgcgat ccgcgcacgc cgtttacctg gcgctgcgaa
    18901 cgcaggttgg cgggcgcagg agcgctggcc gatctgggat cgcatctcat caacatggcg
    18961 cacgttctgc ttggccccat ctcccgcgtc aacggagtgg tccgcaccgt gcacaaggaa
    19021 cgcagtgaaa ccgcgacggg caaggtgcgc acggtggaga acgaggatct cgcgaatgcg
    19081 ctcgtcgaat tcgaatccgg cgtgccggga accctgcaca tcagccgtgt ggcggcgggc
    19141 tacaagtgcg gcctcaccgt gcaggtggtg ggcacgcgcg gcacgctcga gttcaaccag
    19201 gaacgcatga acgaactgcg gctgtatcgc accgatgacg cgacgggtcg ccgcggattc
    19261 cgcaccatcc tcgcgggtcc ggagcatccg gactatggcg ctttctgtcc ggctcccgga
    19321 catggattgg gcatcaacga tctcaaggtg atcgaagtgc gcaacctgtt gcgcgctatt
    19381 cgcaccggca ccccggcgtc accggacttc gccgaaggcc tgcgcgtaca acgagtgatg
    19441 acagctatcg aactcgccgc tgcgtccggc ggctgggtga gcacgcggtg aaatcattcc
    19501 tgcggttctt tgccacctgc gacgacaagc cgccctgtca tgacccgccg acggtcgata
    19561 cgctgttccg gcgtcatcgc ctgcgcatca tgatcgcgat cacgctcggc tacggtgtta
    19621 tctacacctg ccggctggcg ctcggcgtcg tcaagaagcc gctgatcgac gccggcattt
    19681 tcggccccgc cgagctgggc ctgatcgggt cggcactctt ttatacatac gccatcggca
    19741 agctgaccaa cggtttcctg gcggatcacg ccaacatgaa gcgattcctc gcggcgggat
    19801 tcgcgctgag tgcgctgtgc aatatcggca tgggcttctc gaccgcggtc acgttgtcgg
    19861 tggtgctgtg ggggctgaac gggtggttcc agagtctcgg cgcgccgggc ggcgtcgtcg
    19921 ccatgacggc gtggttcagc aatcgcgaac gtgggcgcat gtatggcatc tggagcacgg
    19981 cgcattcgat cggcgagggt ctgaccttcc tggtggtcgg cactatcgtt gcggtactcg
    20041 ggtggcactg gggattcctg attcccgcca tgttcggcct ggcgacggcg gcgctggctt
    20101 actggctggt gcaggaccgg ccccccacca tgggcctgcc cacggtggcc gaatggcgca
    20161 acgatcacta cgtcgccccg gccgtccagc cgaagagcgt attcgcgacg cagctttcca
    20221 tcctgaaaat tcccgcgatc tgggtgctgg cgctgtcgag cgcgactatc tacgtcacgc
    20281 gttatgccat caactcgtgg ggtgttctgt acttgcagga ggcgcgcggc tattcgctgc
    20341 cgatggcggg caccttgctg atgctcagca ccatcgccgg aatggtgggc gcggtggcct
    20401 tcggattcat ctccgatacc cttttcaagg cgcgccgccc gcccgccaac ctgctgttcg
    20461 ccatcctgga attgagcggc ctcctgttga tcttcttcgg cccgcagaac accgccgtga
    20521 ccattgccgg cctcgtgctg tttggcatgg gcctcacagg cctggtcact tcactcggcg
    20581 gcctgttcgc tgtcgacatc tgtccgaagc gcgtcgccgg cgcggcgatg ggtctgatcg
    20641 gcattttcag ctacatcggc gcggccgtgc aggagaacgt cagtggccac ctgatcgagc
    20701 agggcatgaa gatggtcgac ggcgtgcgaa cctacgactt caccaccgtc atcctgttct
    20761 ggatcggctc ctcgttcctc tccatgctgc tggccgccag tctctggcga gtccggctgc
    20821 gcgactaact acagtgcggc gcgggcaacg ccttcacttc atcgtgggca tcacgaactc
    20881 cgccacgctg gtgccgctct tcggccagcg cgacgtcacc gtcttctggc gcgtatagaa
    20941 ccgcactccc tcggggccat gcatgtgcat gtcgccgaac atcgactgct tccatccccc
    21001 gaaactgtgg aacgccatgg gaaccggaat gggaatgttg acgccgacca tgccggcttc
    21061 gacgccacgg gagaactcct gcgccgcgcg accgtcgttg gtgaagatgg ccgcgccatt
    21121 gccgaactcg tgcgagttca ccagctcgag agccgcggcg agcgtaggga cgcgcaccac
    21181 gcacaacacc gggccgaaaa tctcttcgcg atagatacgc atccggggat tcacctggtc
    21241 gaacagcgac gcacccagga aaaatccgtt ctcgaaaccg ggcgccgaaa atccgcggcc
    21301 atcggccacg agcttcgcac cttcctggca gccgagatcg acgtagctgc gcaccttgtc
    21361 gcgatgctcg cgtgtcacca gcggccccat gtccgcgtcg cggtcccggc tttcgcggat
    21421 tcgcagtttc tgcaagcgcc gcgagagcgc cgccacgagg ccatcggcgg agtcgcccac
    21481 ggccacggcg acggagatgg ccatgcaacg ctcgcccgcg gaaccatacg cggcgcccat
    21541 gagcgcatcg gcggcgaagt ccaggtccgc atcgggcatc acgaccgcgt gattcttcgc
    21601 gccgcccagt gcctgcacgc gtttgccgtg ttcgatggcg ccgcgctgca cggccctggc
    21661 cactggcgtg gatcccacga agctgaccgc cttgacgtgc ggatgcgcaa gcaacgcatc
    21721 gaccgcaatc ttgtcgccgt tgatgacgtt gagcacgcca ttcggcgcac cggcttcgag
    21781 cagtagttcg gcaatccgca gcggacagga cggatccttc tccgagggtt tgaggacgaa
    21841 ggtgtttccc gccgcgatcg cgatgggaat catccacaac ggcaccatcg cgggaaagtt
    21901 gaaaggcgtg atacccacgc acacgcccaa cggctggcgc aaggaccagc tgtcgacgcc
    21961 ggtgccgaca tccgccgtga agtcgccctt gagcagctgc ggaatgccgc aggcgaattc
    22021 caccacctcg attccgcgcg tgacttcgcc ctgcgcatcg gacagcacct tgccgtgttc
    22081 ggcactgatc atcgcggcga gttcgtcccg gtgcttctcg agcaatccct tgaactcgaa
    22141 catgacgcgc gcgcggcgca
//
LOCUS       DASQJS010000068         6093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2238612, whole genome shotgun sequence.
ACCESSION   DASQJS010000068 DASQJS010000000
VERSION     DASQJS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2238612"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1714)
                     /locus_tag="VEW08_02625"
     CDS             complement(<1..1714)
                     /locus_tag="VEW08_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYJ39655.1"
                     /translation="MAITSTFNPDAGVLSVLGDSLDNNTDVSRNAAGAILVNGGAVAT
                     VGGTPTVANTTLIQAFGQAGNDVLALSEINGALPAANLFGGAGNDVLTGGSGGDQLFG
                     QDGNDTLLGRGGTDLLFGGEGNDTLTGGDADDQMFGQGGNDRMIWNPGDDTDLHEGGA
                     GVDTSEVNGGNGAEQFTLTANGTRVRFDRVNPAPFSIDIGTTENIVVNMNGGDDSFSA
                     TGNLAALISVTVDGGAGNDTILGSNGADLLLGGDGNDFIDGQQGTDTALLGAGDDVFQ
                     WDPGDGNDTVEGQDGTDTMLFNGSNIGEDFEVSANGSRASFTRNIANINMDLNDVERI
                     DLRALGGADNVVINDLSATDVEEVAVDLSGTAGGTTGDAQLDTVTANATSTSDAIVVS
                     SAGGTVSVNGMAAITTIGNADATDVLRVNGLGGDDIISASTLAPGAISFAASGGDGDD
                     RVTGSSGGDQLSGDAGDDVLLGGGGADVLLGGAGNDVLTGGDGDDQMFGQAGDDRMIW
                     NPGDDSDLMEGGDGNDVAEVNGGNGAEIFTATANGTRVRFDRIDPAPFSIDIGTSERV
                     VVNMN"
     gene            complement(1740..3074)
                     /locus_tag="VEW08_02630"
     CDS             complement(1740..3074)
                     /locus_tag="VEW08_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HYJ39656.1"
                     /translation="MSALIASSLASLLDAPHEPASSARWLNRRVVLIPLALVAATMGI
                     WASLAPISGAVIAAARIKVELERKTVQHREGGIVREIRVRNGQSVRTGDVLVVVDDVR
                     SDAELSLLEDQFRAERVRHARVSAESTLAREFTIDQQLAGDPQSAEHLLRERAQFAAR
                     RRTLDEQLDALQRQVRESGAQAAALTSQVGSIEDSGRLATEELKLSEKLVAEGYLPRA
                     RLLPLQRADADSRSRLAESRGELALARQRAAELHSRMADARNRYQQAATDEQKQSSAR
                     LRELEERLRPSKDQVDRQAIRSPVDGVVMGLRVTATGEVLAAGAAILDVVPSNEMLVV
                     EARIRPQDINHVFADAKAKVRLAAYDSRSTPMLAGRVTFVSPDRMSDAATGESWFAAT
                     VEVDVAALKHHPEIQLKAGMPAELFVTTSHRTLFEYLVSPITAFTNRAMRET"
     gene            complement(3071..4765)
                     /locus_tag="VEW08_02635"
     CDS             complement(3071..4765)
                     /locus_tag="VEW08_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HYJ39657.1"
                     /translation="MDWLFAQQIRPFMKLAAQASLLLNLALLAPALYMLQVFDRVFAS
                     GSIETLVMLSVPLLIMLVLGYYMDAARARTLAAAGRRVEACLAPAALGNALQQAAAGQ
                     RHDQEALRDVAQLRSLLASPGVVALFDAPWVTIYLLIITLMHPLLGGIAALGAVSLFA
                     LGVFTEYATRRHTERALAAARATHRQADLLMRNAESVVGMGMTTRAIETWQQCCDDQH
                     EAQEQLAGISARLGSLARMSRQGLQAVALGFGAWLVIGREASPGIMIAATILLGRALQ
                     PVELLIGGWKAMIEARGAWRRLGERQSHQRQAPLALPPPKGRLDVERLVFSPVAGRAP
                     LIRGVAFTLFAGDSLGIVGPSASGKTTLVRLLLGIRAPQTGKVRLDGADISRFDRDLL
                     GRAVGYLPQEVQLFAGSVAANIARLQKPDPQAVIRAAQLAQAHDLILRLPEGYDTEIG
                     DGGCLLSGGQRQRIALARALYGDPRLVILDEPNANLDEEGDMALAAALAGLKARGVTV
                     ILVGHRQSIMKQLDKLAVLNKGVFESFGPSAIILARLREGAKIVQFPAAESLQVQA"
     gene            complement(5330..>6093)
                     /gene="rapZ"
                     /locus_tag="VEW08_02640"
     CDS             complement(5330..>6093)
                     /gene="rapZ"
                     /locus_tag="VEW08_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HYJ39658.1"
                     /translation="AQYSRAAVGVDARNAAAEIETVPPLIEELKKSGIQCEIIFLLAN
                     DEELLRRFAETRRKHPISTDHVSLKEAIAIERRLLEPILNAADLVIDTSRMGVHDLRE
                     LISQRVEKRSAGRLSLLVESFGFKNGIPGDADFVFDARSLPNPYWDPSLRALTGRDAD
                     VIRFLEAQRSVPTLAEDILQFVSARIPEHQAANRRYLTIAIGCTGGRHRSVYLAERIA
                     ERLSHNYPVVGIRHNSLGETRSITRAPTLAGAQSA"
BASE COUNT          975 a         1973 c         2033 g         1112 t
ORIGIN      
        1 cattcatgtt cacgaccacg cgctcgctgg ttccgatatc gatcgagaac ggagccgggt
       61 cgatccgatc gaaacgcacg cgcgtgccgt tggccgtggc cgtgaaaatt tctgcgccat
      121 tgccgccatt cacttccgcg acgtcgttcc cgtcgccgcc ttccatgagg tcgctgtcgt
      181 cgcctggatt ccagatcatg cgatcgtcgc ccgcctggcc gaacatctgg tcgtcgccgt
      241 cgccgccggt caacacgtcg ttgcccgcgc cgccgagtaa cacgtcggca ccgccaccac
      301 ccagcagcac atcgtcgccg gcgtcgccag acagctggtc gccgcccgag cttcctgtca
      361 cgcggtcatc gccgtcaccg ccagatgcgg cgaagctgat ggctcccgga gcgagcgtcg
      421 acgcgctgat gatgtcgtcg ccaccgagtc cgttgacacg cagcacgtcg gttgcatcgg
      481 cattcccgat cgtcgtgatc gccgccattc cgttgaccga gaccgtgcca ccagcgctgc
      541 tcaccacgat ggcgtcggag gtgctcgtgg cattcgccgt caccgtgtcg agttgggcgt
      601 cgccggtcgt gccgccggcg gtgccggaga gatccacggc aacttcctcg acgtcggtgg
      661 cggagaggtc attgatgacc acattgtccg cgccgccgag cgcacgcaga tcgatacgct
      721 ccacgtcgtt gaggtccatg ttgatgttcg cgatgttgcg cgtgaagctg gcgcggctgc
      781 cgttggccga gacctcgaag tcttcaccga tgttggaacc gttgaacagc atggtgtcgg
      841 tgccatcctg gccctcgacc gtgtcgttgc catcgcccgg gtcccactgg aatacgtcat
      901 cgcccgcgcc taacaacgcg gtatcggtgc cctgctggcc gtcgatgaag tcgttgccat
      961 cgccaccgag cagaagatcg gctccgttgc tgccaagaat cgtgtcattg cctgcgccac
     1021 cgtccacggt cacggaaatc agtgccgcga gattgcccgt ggcggaaaac gagtcgtcgc
     1081 cgccgttcat gttcaccacg atgttttcgg tggtgccgat gtcgatggag aagggcgcag
     1141 gattcacgcg gtcgaagcgc actcgcgtgc cgttggcagt cagcgtgaac tgctccgcgc
     1201 cgttgccgcc gttgacctcg ctcgtgtcga cgccggcgcc accttcgtgc aggtcggtgt
     1261 cgtcgccggg attccagatc atgcggtcat tgccaccctg gccgaacatc tggtcgtcgg
     1321 catcgccgcc ggtcagcgtg tcgttgccct cgccgccgaa cagcaggtcg gttccaccgc
     1381 ggcccagaag tgtgtcgttg ccatcctggc cgaagagctg gtccccgccg gagccgccgg
     1441 tcagcacgtc gttgccagcc ccgccgaaga gattggcggc aggcagcgct ccattgattt
     1501 cactgagcgc gaggacgtca ttgccagcct ggccgaacgc ctgaatcagc gtcgtgttgg
     1561 cgaccgtggg tgtgccacct accgttgcga cggcgccgcc gttgacgagg atggcgccgg
     1621 ccgcattgcg gctgacatcg gtgttgttgt cgagcgaatc gccgaggacg ctcagcacgc
     1681 cggcgtcggg attgaaggtg cttgtgatgg ccatgaaaag ctctccgtag ctggttggtt
     1741 caggtttcgc gcattgcgcg gttcgtgaat gccgtgatgg gactcacgag gtattcaaaa
     1801 agggtgcgat gggacgtggt cacgaacagc tcggccggca tgccggcctt gagctggatc
     1861 tcgggatgat gtttgagcgc ggcgacgtcc acctcgaccg tggccgcgaa ccacgattcg
     1921 ccggttgccg cgtcgctcat gcgatcgggt gagacgaaag tcacgcggcc cgccagcatc
     1981 ggcgtgctgc gtgagtcgta ggccgccagg cgcaccttcg ccttcgcatc ggcgaagaca
     2041 tgattgatgt cctgagggcg gattcgcgct tccacgacca gcatttcgtt gctcggtact
     2101 acgtcgagga tggctgcgcc tgcggcgagc acttcgccgg ttgccgtgac tcgcagaccc
     2161 atgaccacgc cgtccaccgg cgagcgaatg gcctgccggt ccacctggtc tttcgaggga
     2221 cgaagtcttt cttccaactc gcgcaatcgg gccgatgact gcttctgctc gtcggtggcc
     2281 gcctgttgat aacgattgcg cgcgtcggcc atgcgcgagt gcagttcggc ggcgcgctgt
     2341 ctcgccagcg cgagctctcc gcgcgattcc gccaggcggc ttcgcgagtc ggcgtcggcg
     2401 cgctgcagag gcaacagccg cgcgcgcggc aggtagcctt cggcgaccag cttctcgctg
     2461 agtttgagtt cctcggtggc gagccgcccg gagtcttcga tggacccgac ctggctcgtg
     2521 agtgccgcgg cctgtgcgcc ggactcgcgc acctgtcgtt gaagcgcgtc gagctgctcg
     2581 tcgagtgtgc ggcgccgcgc ggcaaactgc gcgcgctcac gtagcaggtg ctcggcgctt
     2641 tgcgggtcgc cggccagttg ctggtcgatg gtgaattccc gtgcgagcgt gctctcggcg
     2701 ctcacgcgtg catggcgcac gcgctcggcg cgaaactggt cttcgagcag gctgagctcg
     2761 gcgtcgctgc gcacgtcatc cacgaccacc agcacatcgc cggtgcgcac cgactgaccg
     2821 ttgcgcacgc ggatttcgcg cacgatgccg ccttcgcggt gctggacggt cttgcgctcc
     2881 agctccacct tgatgcgcgc ggcggcgatc accgctcccg atatcggcgc cagcgacgcc
     2941 cagattccca tcgtggcggc caccaaggcc agcggaatca gcacgacgcg gcgattcagc
     3001 cagcgcgcgg agctggcagg ttcatgcggc gcatcgagca gcgatgccag gctggatgcg
     3061 atcagggcgc tcatgcctgc acctgcagcg attcggcggc gggaaattgc acgatcttcg
     3121 cgccttcgcg aagccgcgcg agaatgatgg ccgacggacc gaatgactcg aacacgccct
     3181 tgttcagtac ggcgagcttg tcgagctgct tcatgatgct ttggcgatgt ccaaccagta
     3241 tcacggtgac gccgcgcgcc ttgagcccgg ccagtgccgc ggccagcgcc atgtcacctt
     3301 cttcatcgag attggcgttc ggctcatcga gaatcaccag ccgcgggtcc ccgtacagcg
     3361 cgcgcgccag cgcgatgcgc tgccgctggc cacccgatag caggcagccg ccatcgccga
     3421 tctcggtgtc gtaaccctcg ggcagccgca ggatgagatc gtgcgcctgc gccagttgcg
     3481 cggcgcggat caccgcctgc ggatccggct tctgcaggcg cgcgatgttg gcggccacgc
     3541 tgccggcgaa cagctgtact tcctgcggta gatagcctac ggcgcggccg agcaggtcac
     3601 ggtcgaagcg cgagatgtcg gcgccatcga ggcgcacttt gcctgtttgg ggcgcgcgga
     3661 ttccaagcag caggcgcacc agcgttgtct tgccggatgc gctgggtccg acgatgccga
     3721 ggctgtcgcc ggcgaacaag gtgaaggcga cacctcggat caatggtgcg cgcccggcta
     3781 ccggcgaaaa aaccagccgt tcgacgtcga gccgtccttt cggtggcggc agggcgagag
     3841 gcgcctggcg ctggtgtgat tgccgttcgc ccaggcggcg ccaggcgccg cgggcttcga
     3901 tcatggcctt ccatccgccg atgagcagct ccacgggctg cagcgcgcgg cccagcagaa
     3961 tcgtggcggc gatcatgatg cccggtgagg cttcacggcc gatcaccagc caggcgccga
     4021 acccgagcgc caccgcctgc aagccctggc gcgacatgcg tgccagcgag cccagtcttg
     4081 cgctgatacc ggcgagctgc tcctgcgcct cgtgttgatc gtcacaacat tgctgccagg
     4141 tttcgatcgc gcgcgtggtc atgcccatgc cgaccacgga ttcggcgttg cgcatcagca
     4201 ggtccgcctg ccgatgcgtc gcgcgcgccg ccgccagcgc ccgttcggtg tgcctgcgcg
     4261 tggcgtattc ggtgaagact cccagcgcga agagcgagac cgcgccgagg gcggcgatgc
     4321 ctccgagcaa cgggtgcatg agcgtgatga tcagtagata gatcgtcacc cagggcgcgt
     4381 cgaacaacgc caccacgccg gggctggcca gcagcgagcg cagctgcgcg acgtcgcgca
     4441 acgcttcctg gtcgtggcgc tggccggcgg ccgcctgctg cagggcgttg cccagggctg
     4501 cgggtgcgag gcaggcttcg actcgccgcc cggcggcggc cagcgtgcgc gcacgtgccg
     4561 cgtccatgta gtagccgagc accagcatga tgagcagcgg tacgctcagc atcaccaggg
     4621 tctcgatgct gccgctggcg aaaacgcgat cgaatacctg cagcatgtag agcgcggggg
     4681 ccagcagcgc caggttcaat agcaggctcg cctgtgcggc gagcttcatg aacggacgaa
     4741 tctgttgcgc gaagagccag tccattgcca tctcccaatt gctgtcggga ggtgagtggc
     4801 cgggcgcggg cacgcggttc cgttaaatgg ggacattcct cgtttcttag caaacgggga
     4861 cgcgccaacg tcgtgcctcg atcccgcttg caaagaaata ggagattggg acctcaatgt
     4921 tcgaggcgcg tccccaatgc ccagcggcgg tggtaagaga agagacgcac ggtcttgagg
     4981 tcttcccaca gcgtggggtt acacgcctct ttttctggcg aacggagcgg cggcgcgtcg
     5041 gtgcacgatc gagcgacggg tggccctgca tgcgatgagc gagtgggccg ggatacctgg
     5101 cctcgttagg cacgaagccg attcctgcgt cttcgggact cttcgccatc ttcaaatcaa
     5161 accacgccag agtgtctgcg tgtgtgtcgg cggcccgcaa actggcggca agcaactaac
     5221 gaagggcccg gcgaaaagcg agtctcatcg catgcagggc cgcccgtcgc tcgaccctcc
     5281 acatacgagc cacgtcccct ttatccaagc aaaagaggca tgtccccatc taagcggatt
     5341 gggcgcccgc gagcgtcggt gcgcgcgtga tggagcgcgt ctcgccgaga gagttgtgcc
     5401 ggatgccgac gactggatag ttgtgcgaca gccgctctgc gatgcgttcg gccaggtaca
     5461 ccgagcgatg ccgcccgccg gtgcagccga tggcgatggt gaggtaacgg cggtttgccg
     5521 cctggtgttc cggaatgcgc gcggacacga actgcaggat gtcttcggcc agggtcggaa
     5581 cgctgcgttg cgcctcgaga aagcggatga cgtccgcatc gcggccggtg agggcgcgca
     5641 acgacggatc ccaatatgga ttgggcagcg aacgcgcgtc gaacacgaag tctgcgtcgc
     5701 ccggaatgcc attcttgaag ccgaacgatt ccaccagcag cgacagccgt ccggcgctgc
     5761 gtttttccac gcgctggctg atgagctcgc gcaggtcgtg tacccccatg cgcgaggtat
     5821 cgatgaccag gtctgcggca ttgaggatgg gttcgagcag gcgccgctcg atagcgatgg
     5881 cttccttcag cgagacgtgg tccgtgctga tggggtgctt gcggcgcgtt tcggcgaaac
     5941 gccgcagcag ctcttcgtca ttggcaagca ggaaaatgat ttcgcactgg atgccgcttt
     6001 tcttgagctc ttcgatgagc ggcgggacgg tttcgatttc cgcggcagcg ttgcgcgcat
     6061 cgactcccac cgcggcgcgc gagtattgag cgt
//
LOCUS       DASQJS010000069         4482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4436776, whole genome shotgun sequence.
ACCESSION   DASQJS010000069 DASQJS010000000
VERSION     DASQJS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4436776"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VEW08_02645"
     CDS             complement(<1..703)
                     /locus_tag="VEW08_02645"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HYJ39659.1"
                     /translation="MKFLLTLLFCLMAGGCATLPSGKADPRDPWERFNRASFKFNDTL
                     DRAIARPVAKAYVKVMPRVIRTGVSNALSNLDNVPTIINDTLQGKFSQAGRDSARFLL
                     NSTLGLGGLFDPASAAGLEYNDEDLGQTLGKWGVKPGPYIMLPILGPSSVRDTFSRAA
                     DSFMEPVWYLEDDSTRYIIRLIDLLDQRAGLLELDSQLGRSYDRYAFIRNAWLQRREY
                     QVKDGNVEDQSLELEE"
     gene            complement(700..2244)
                     /locus_tag="VEW08_02650"
     CDS             complement(700..2244)
                     /locus_tag="VEW08_02650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HYJ39660.1"
                     /translation="MPTGLKKLIVALLSVLLPLQSLAQTGNDLPDIGSPASSALTLDD
                     EYHIGLQVMRQLRDEGQIIEDPEATEYLQSLGSRIVAQATGDSAQRFSFFFVNDDTIN
                     AFALPGGFIGVNYGLVLATRNEAQLAGVLAHEIAHVTQRHIARRIHSQGRQSIGTMAA
                     ILAAILIGAATGSSDAALGTISMASGAAMQQQINFTRANENEADRVGMSFLAAAGFDP
                     YGMPDFFETMGRRTPLANTNRNALPEILQSHPITTNRIAESRSRAAQFKEIKPTAETV
                     SYALTRERLRVLATPTEDNVRRYYSERRDQQQPTAGERYGEALASYQGGSARAALDSL
                     TELARQYPQVPMLQSTLGQALMSAGDTEESLAVFRRALNLSPRNIPLTMRYADALMKA
                     DQAKAAHAVLLDLFNNVAPTPEQIRFTALAASSAGDTGDAAYYMSEYHIATGNLPLSV
                     AQLEMALAAPNITVVQRSRYQARLDEVREALFSDRKRRKNQEQQKKKDEQTQRSGAGS
                     AGTISR"
     gene            complement(2303..3775)
                     /locus_tag="VEW08_02655"
     CDS             complement(2303..3775)
                     /locus_tag="VEW08_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HYJ39661.1"
                     /translation="MTTGKRSTGGKSTKPDVERRLFVLDTNVLMHDPTAIFRFQEHDV
                     YLPMVVLEELDSNKKGLSEASRNVRQTSRFLDDLMRDATKDQIDAGLPLPSAMNGPGT
                     GNGHGGGKVGLGKAGRLFFQTRQLTSALPDYLPGHGADNAILAQTLALQALHHEARVT
                     LVSKDINLRIKAAVVGVHAEDYYSDKTIEDADVLYTGLAALPADFWEKAGKDIKSWKE
                     GGRTFYQVTGPMVAELHVNQFVHEAGEAGIEAIVRRIEAGTATLELARDFRGERHSVW
                     GVNARNREQNFALNLLMDPEIDFVTILGPAGTGKTLLTLAAGLMQTLESNRFAEIIMT
                     RVTIPLGEDIGFLPGTEEEKMEPWMGALMDNLEVLTQTGEGGNWGRAATNDLLRNRIK
                     IRSLNFMRGRTFLNRYVILDEAQNLTPKQMKALVTRAGPGTKVICLGNIAQIDTPYLT
                     ETTSGLTYVVNRFRGWGHSGHITLVRGERSRLADFASDTL"
     gene            complement(3772..4248)
                     /locus_tag="VEW08_02660"
     CDS             complement(3772..4248)
                     /locus_tag="VEW08_02660"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYJ39662.1"
                     /translation="MSVAVGKKVPAFSLPRSDGSMWTTDALSTDGKGGKLVLYFYPKD
                     MTPGCTLEGQDFRDLHAAFKRAKTTVLGVSKDSCERHAKFRAREKFPFELLSDEDEKV
                     CRLFDVIREKSLYGRKFMGIERSTFLIDEGGKLVREWRKVRVKGHAAEVLEAAKSL"
     gene            complement(4245..>4482)
                     /locus_tag="VEW08_02665"
     CDS             complement(4245..>4482)
                     /locus_tag="VEW08_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39663.1"
                     /translation="LDQAGIVTGLSGFCASRGIDIAEVSTRTYPAAHTGAQMFAVQMI
                     INVPKRLQLAHLREEFMDYCDAQNLDAILEPVKS"
BASE COUNT          738 a         1330 c         1514 g          900 t
ORIGIN      
        1 cctcttccag ttccagcgac tggtcttcga cgttgccgtc tttgacctgg tactcgcgcc
       61 gctgcagcca ggcattgcgt atgaacgcat aacggtcgta actgcgcccc agctgtgaat
      121 cgagctccag caggccggcg cgttgatcga gcaggtcgat caaccgaatg atgtatcgcg
      181 tgctgtcatc ttcgaggtac cagactggct ccatgaagga gtcggccgcg cgggagaacg
      241 tatcacgcac cgaggagggc ccgagaatgg gcagcatgat gtagggcccc ggcttgacgc
      301 cccacttgcc cagggtctgc ccgagatctt cgtcgttgta ttcgagcccg gccgcgcttg
      361 ccggatcgaa taacccgccc agcccgagcg tgctgttcag caggaagcgc gccgagtcgc
      421 gccctgcctg ggaaaacttg ccctgcaggg tgtcgttgat gatggtgggc acgttatcga
      481 gattgctgag cgcattgctg acgccggtgc ggatcacgcg gggcatcacc ttcacatagg
      541 ccttggccac gggcctggca atggcacggt cgagtgtgtc gttgaacttg aaggaggcgc
      601 ggttgaaacg ctcccagggg tcgcgtggat cggctttgcc ggagggcaac gtggcgcaac
      661 ctcctgccat caggcagaag agcagggtga gcagaaattt catcgcgaaa ttgtaccagc
      721 cgaacctgcg ccagaacgct gggtttgctc atccttcttc ttttgctgtt cctggttctt
      781 gcgccgcttg cgatccgaga acagcgcttc gcgcacttcg tcgagccgtg cctggtaccg
      841 gctgcgctga accaccgtga tattgggtgc cgccagtgcc atttcgagct gcgccaccga
      901 cagcggtaga ttgcccgtgg cgatgtggta ctcgctcatg taataggcgg cgtcgccggt
      961 gtcgccggcg ctgctggccg caagcgcggt gaaccgaatc tgctccggcg tcggcgcgac
     1021 attgttgaag aggtcgagca gcaccgcgtg cgcggccttc gcctggtcgg ctttcatcag
     1081 ggcgtcggca taacgcatgg tgagcggaat attgcgcggc gacaggttga gcgcgcgacg
     1141 gaagacggcg agggactctt cggtatcacc cgcgctcatg agcgcctgtc ccagagtcga
     1201 ctgcagcatg gggacctgcg gatactggcg cgcgagctcg gtcagcgaat cgagggcggc
     1261 gcgcgcgctg ccgccctggt agctagccag cgcttcgccg taacgctcac ctgcagtcgg
     1321 ctgctgctgg tcgcgccgtt cggagtagta acgccgcacg ttgtcttccg tcggggtcgc
     1381 gagcacgcgc aggcgctcgc gggtcagcgc gtaactcacc gtctcagcgg tgggtttgat
     1441 ctccttgaat tgcgcggcgc gcgagcgcga ctcggcgatg cgattggtcg tgatcgggtg
     1501 cgactggagg atttccggta gcgcgttgcg gttggtgttg gcgagcgggg tgcgacggcc
     1561 catggtttcg aagaagtccg gcatgccgta gggatcgaaa ccggccgcgg cgaggaagct
     1621 catgccgacg cgatcggctt cgttctcgtt ggcgcgcgtg aagttgatct gctgctgcat
     1681 ggccgcgccc gaggccatgg agatggtgcc gagcgcggcg tcggaggagc cggtggccgc
     1741 accgatcagg atggccgcga ggatggccgc catggtcccg atgctctgcc ggccctgcga
     1801 gtggattcga cgtgcgatgt gccgctgggt gacgtgcgca atttcgtggg cgagcacgcc
     1861 agccagctgc gcttcattgc gggtggcaag taccaggcca tagttcactc cgatgaaccc
     1921 gccgggcagc gcaaacgcgt tgatggtgtc gtcattcacg aagaagaacg agaaacgctg
     1981 cgccgaatcg cccgtggcct gcgcgacgat gcgcgagccc agcgactgca ggtattcggt
     2041 ggcctcgggg tcttcgatga tctggccttc gtcgcgcagc tggcgcatga cctgaaggcc
     2101 gatgtgatat tcgtcgtcga gcgtgagggc tgacgaggcg gggctgccga tgtcgggcaa
     2161 atcgttgccg gtctgcgcga gcgattgcag cggcagcagc accgacaaca gtgcgacgat
     2221 gagcttttta agtccggttg gcatcgcgga attggacccg gcgtccgccc tttaagtttc
     2281 tggccggtta tggcaggggg cgctagagcg tatcagaggc aaaatcggcg aggcgggacc
     2341 gttcgccgcg aaccagggtg atgtgccccg aatgccccca gccacggaag cggttcacga
     2401 cgtaggtcaa accggaggtg gtctcggtca ggtacggcgt gtcgatctgc gcgatgttcc
     2461 cgaggcatat gactttcgtg ccggggccgg cgcgagtcac gagcgccttc atctgcttgg
     2521 gagtcaggtt ctgggcttcg tcgaggatga cgtaacggtt caggaaggtg cgtccgcgca
     2581 tgaaattgag cgaccggatc ttgatgcggt tgcgcagcag gtcgttggtg gcggctctgc
     2641 cccagttgcc gccttcgccc gtctgagtga gcacctcgag gttgtccatg agggcgccca
     2701 tccacggctc cattttctct tcctcggtgc cgggcaggaa gccgatgtct tcgccgagcg
     2761 ggatcgtcac gcgcgtcatg atgatttcag cgaagcgatt cgattccagc gtctgcatga
     2821 gtccggcggc gagcgtgagc agcgtcttgc cggtgccagc ggggccgagg atggtgacga
     2881 agtcgatttc cggatccatg agcaggttca gcgcgaaatt ctgctcgcgg ttgcgggcgt
     2941 tcacgcccca cacgctgtgg cgctcgccac gaaaatcgcg cgccagttcc agcgtggcgg
     3001 tgccggcttc gatgcgccgg acgatcgcct cgattccggc ttctccggct tcgtgcacga
     3061 actggttgac gtgaagctcg gccaccatcg gcccggtcac ctgataaaag gtgcggccgc
     3121 cttctttcca ggacttgatg tccttgccgg ccttctccca gaaatccgcc ggcaatgccg
     3181 cgagccccgt gtagaggacg tcggcgtctt cgatggtctt gtcgctgtag tagtcctcgg
     3241 cgtggacacc gaccacggcc gccttgatgc gcaggttgat gtccttggaa accagcgtga
     3301 cgcgcgcttc gtgatgcagt gcctgcaatg ccagggtctg cgcgaggatg gcgttgtccg
     3361 cgccatgacc gggtagatag tcgggcaggg cggaggtgag ctggcgcgtc tggaagaaga
     3421 ggcggccggc ttttccgagc ccgactttgc cgcccccgtg cccgttgccg gtgcctggcc
     3481 cgttcatggc cgaaggcagg ggaaggccgg cgtcgatctg gtccttggtg gcatcgcgca
     3541 tcaggtcatc gagaaagcgg ctcgtctggc gcacgttgcg cgacgcctcc gagagaccct
     3601 ttttgttcga atcgagctct tcgagcacca ccatcggcag gtacacgtcg tgttcctgga
     3661 accggaagat ggccgtgggg tcgtgcatga gcacgttcgt atcgagcacg aacagccggc
     3721 gttccacgtc cggtttcgtc gacttgccgc cagtcgaacg cttgcctgtc gtcacagtga
     3781 tttggccgcc tctaacactt cggcggcgtg gcccttgacg cgaaccttgc gccactcacg
     3841 gaccagcttg ccgccttcgt cgatcaggaa agtactgcgc tcgattccca tgaacttcct
     3901 tccatataga gatttctcgc ggatcacgtc gaacagccgg cagactttct cgtcctcgtc
     3961 ggaaagcagt tcgaagggaa acttttccct ggcgcggaat ttcgcgtggc gttcgcagct
     4021 gtccttggag accccgagca ccgtggtctt cgcgcgtttg aaggccgcat gcaggtcacg
     4081 gaaatcctgg ccttcgagcg tgcagcccgg cgtcatgtcc ttggggtaga agtagagcac
     4141 cagtttgccc cccttaccat cagtggagag ggcgtcagtg gtccacatgc tgccgtccga
     4201 acgcggcagc gagaacgcgg gcactttctt gccgacagca acgctcaaga cttgaccggc
     4261 tccaggatcg catcgaggtt ttgcgcgtcg cagtaatcca tgaattcctc gcgaaggtga
     4321 gcgagctgca ggcgtttggg cacgttgatg atcatctgca ccgcgaacat ctgcgcgccc
     4381 gtgtgcgccg cggggtaggt gcgggtggag acttcggcga tgtcgatgcc gcggctggcg
     4441 cagaacccgg agagcccggt gacgattccg gcctggtcca gg
//
LOCUS       DASQJS010000070        16416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1296466, whole genome shotgun sequence.
ACCESSION   DASQJS010000070 DASQJS010000000
VERSION     DASQJS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16416
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296466"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2168)
                     /gene="thrA"
                     /locus_tag="VEW08_02670"
     CDS             complement(<1..2168)
                     /gene="thrA"
                     /locus_tag="VEW08_02670"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HYJ39664.1"
                     /translation="MQSADPSAWVVHKFGGSSVADAECFKRVANILETLPPGRLGIVL
                     SACRGVTDALLGLVARAEAQDDAWRGELDALRVRHNTIAEALLSGDARQLYMAGFDRD
                     RHDIEGILHTVKLTRSAAQNVRDLIAGYGEIWSTKLFREFFSARAKRPGDVKWVDARQ
                     AVVVEWGPLGPAVQWEESRAKLSALVATSAKDGAFKGTLIITGFIASDRRGVQTTLGR
                     NGSDFSGSIFGSLLDASEIHIWTDVDGVLSADPRRVPDAKVIDSLSYNEAMELAYFGA
                     KVIHPQTMAPAIGRGIPIWIRNTFAPQKPGSLICAKPDSKLPVKGITSIENIAMVNVE
                     GAGMIGVPGTAHRLFGALREEGISVILISQGSSEHSICCAIPGVEADRAARVVAAAFD
                     REIKEGQIQSIAVDRDLAILAVVGDGMAGLPGVSGQVFNALGSAAVNVHAIAQGASER
                     NISVVVDGKDATRALRAVHAGFYLSPHTISIGLIGPGSVGRALLEQIAAESSRLRDDF
                     KLDLRLRGIMGRKKMLLAEQGIAVDGPKDSWRAALDASPHAADIGKFVDHLHVDHLPH
                     TVIIDCTADDSVAKHYAEWLTAGIHVVTPNKKANSGPLAYYESLKEARRLGGSSYLYE
                     ATVGAGLPVIQTLRDLRETGDKITSVEGIFSGTLAYLFNVYDGKTPFSDIVKDAKAKG
                     YTEPDPRDDLSGTDFVRKVIILGREMGLKLEMKDVQVESL"
     gene            2284..3312
                     /locus_tag="VEW08_02675"
     CDS             2284..3312
                     /locus_tag="VEW08_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HYJ39665.1"
                     /translation="MSLHRLLPLYDRALLANEPLVLATVIRTGGSTYAKPGAQMLIAA
                     DGEYAGLLSGGCLEGDLREHARHVAASGQARIVSYDLRSTTDQLFGLGAGCEGAMDVL
                     LSRVSAAEGWQPLVAMAESFRAAREQHFAFVIASTDPAYPLGVSFPVNGQVTPYPSGV
                     EVFVAAVAPPPHLLLLGGGPDARPVATLAAFLGWRITVVEHRAVYLAPDRFPPSTRLI
                     ETRPDNVAAAVRLAEFSAAIVMSHHLESDLHYLRALARAPVPYVGLLGPAARREKLLS
                     DLGEDAGTLRPRLRAPVGLDIGGRTPESIALAIVGEVHAALAGRAGRPFSETLKSDST
                     ASADGSER"
     gene            3314..4123
                     /locus_tag="VEW08_02680"
     CDS             3314..4123
                     /locus_tag="VEW08_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYJ39666.1"
                     /translation="MEELLPLIAILAVSGLIAGFAAGLLGVGGGIVTVPVLEYSLRFA
                     DVPEQYRMHVAVATSLAAIIPTSISSASTHHSRGAVDWELARRWAAPMFVASFAGSLL
                     ASRAPLAVLAGVFGSVALLIALKMLLPFDHWRARESVPRGAGGAALASLIGGVSAVMG
                     IGGGTLTVPTLNLCGYPIHRAVGTASFFGIVISIPGTVGYLLAQSPVALPWATVGFVS
                     VVGLAIITPGSMLTATLGARVAHSLSRRRLSQAFGVFLLLVGSRMIYRALA"
     gene            complement(4105..6234)
                     /locus_tag="VEW08_02685"
     CDS             complement(4105..6234)
                     /locus_tag="VEW08_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYJ39667.1"
                     /translation="MTTQFFERQDAQRKYTLWLVSGFVAAILLVVLVVNLVVILGLGA
                     DPRWVLRHEPHVVFWITLVVFGTILIATWHRSSQMREGGPAVARALGGVQVNANDGDL
                     KRQRLVNIVEEMAIAARIRKPQVFVLPDEAGINAFAAGLSPDEAAVAVTQGALDALDR
                     DQLQAVIGHEFSHVLNGDMKINMRLTAWIFGLCVITDLARRFMNRRGGGKSEARIKLI
                     ALGVFLAGSAGAFAGRLLQAAVSRRREHLADASAVQFTRNPQALQSAFIVMAASASGT
                     RLEHEHAIGVSHMFFAGSSPAWITKFKASWFATHPPLDERVRAIDARITPLKFRSLVN
                     EEKRRIATRAAAAAATAPAAEPTAKGDVASESSNALQFSPVAAMAAGPVPATASRARA
                     PARAAPAPTTSAADSGIHLTLEKPILAETLPTGVRMVAGRALPPDVLRNRLSQDQQAA
                     IGTFVARVELSGIAVQATFVATMLASEPAKWRTQLTRLAPLLGIELFKETQAQVARVG
                     ELAPASRLPLLVDLFSLLDDMQPADRKRLRAIARAFAPTVATGDMLRFSLTRMIEKRL
                     AKAREEAPPVPLHEQAPMVCELYAALAQCRFGAGKQGQNAYRAGLMGMLPPTKWAPYP
                     EQPITPAALDSALTAVAQIHPTGRRSLSEGMARVIAVGGRLTVPQVDLLRGICMVVDC
                     PVPLIPIDVVFEEVDVTAPKNVQASAR"
     gene            6400..6624
                     /locus_tag="VEW08_02690"
     CDS             6400..6624
                     /locus_tag="VEW08_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39668.1"
                     /translation="MNLEPQKSESEIRNYSGVARILASVTILALAATTILVVLEIIPR
                     STFAEMAGKIAMVAGVCVVSALAIGFLSRR"
     gene            6621..7262
                     /locus_tag="VEW08_02695"
     CDS             6621..7262
                     /locus_tag="VEW08_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYJ39669.1"
                     /translation="MNESGSTESSHIHVVVLAAGNSSRFGSPKQLVRVNGRPLMHTVV
                     SRAVELAGHSVTVVLGANAAELAPLLRHSPAGIAVNRDWAEGIASSIREGIAHAPQAA
                     DGVLIALADQVAVTTEDLRRLAGVWRRTPASIAAAQYAGAVGVPAIFPRWCFRELNEL
                     RGDHGAQLLLIRHVDRLIRLPMPSAELDIDRPEDLLALEANTELKRGETPEAE"
     gene            complement(7284..8024)
                     /locus_tag="VEW08_02700"
     CDS             complement(7284..8024)
                     /locus_tag="VEW08_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYJ39670.1"
                     /translation="MPLFALVILAAVMFGLSVFGLRGLMDIREALRSADLNRLASPAH
                     WQVTRAWLGLAAAVPVFFTGLANGAWLAAAAVAGLGYWVAPRFLASARQRVEHELLDD
                     LPVHLDLIALAMEAGWALPAALATCAHRAPDGALRRAWMRVVLDIHGGAEPLEALRAM
                     EQRTGLKPLAILVTALRSAEKFGIPAAQVVRERARQSAAQRFARAERLARAAPLHLWA
                     ALVLTIVPCTFVVLAFPLARMLARIVGQ"
     gene            complement(8025..8540)
                     /locus_tag="VEW08_02705"
     CDS             complement(8025..8540)
                     /locus_tag="VEW08_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39671.1"
                     /translation="MPFDGVLPGSMVVFAAFIALPALAATSRRRAMCEASDVACALGA
                     LGVAVRKSGACLPVDSALCKRVQRLRAPEFVSLLLVQELPHPTPQLLADTAQRLALRL
                     KRRVAFERKMLARTASGRRRGAVAASVPPLALLALDAAGIQVPLAALLFVVALEACGC
                     WLLWRMARVAI"
     gene            complement(8530..8682)
                     /locus_tag="VEW08_02710"
     CDS             complement(8530..8682)
                     /locus_tag="VEW08_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39672.1"
                     /translation="MDAQQLKVRARHVSRPLTRLVVAWLAAVLAIGLWQGYGRWLLTG
                     GVGDAI"
     gene            complement(8795..9352)
                     /locus_tag="VEW08_02715"
     CDS             complement(8795..9352)
                     /locus_tag="VEW08_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39673.1"
                     /translation="MQVENPQLGFPTSFCCNCGATECKSEIQDTRITRFFGIGRTDTT
                     FKLSVPICAGCQRTIRRKPAGFFSRLGVLLLITGVIFGICLAAGSTVALPLWIDYMYA
                     GSVVLGVVLTILFYRLRRPKAPQTSFYQPVRIKNVRLQFTGLMNGEGQVGFMKLAFTN
                     PDYLNAFVAANQDAITAKRVSVTKA"
     gene            complement(9371..10879)
                     /locus_tag="VEW08_02720"
     CDS             complement(9371..10879)
                     /locus_tag="VEW08_02720"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004951467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HYJ39674.1"
                     /translation="MTHERVSSYSHWIGGQLVAGTSGRAGDVFNPSLGVVAARVALAN
                     EADVDAAVRAARAAFPAWAATPALKRARVLFKFKEIIEQRRDALAALIASEHGKLVSD
                     AAGEVTRGLEVVEFACGAPHLLKGEFADNVGTGVDSYSLRQPLGVAAGITPFNFPAMV
                     PLWMFPVALACGNCFILKPSERDPGPGLLLAQWLKEAGLPDGVFNVVNGDKVSVDALL
                     RHPDVNAVSFVGSTPIAEYVQREGVAHGKRVQALGGAKNHLVMLPDADTTQVCDALLG
                     AAYGSAGERCMAISVAVAVGDATADRLVGALAERVRGLRVDQSQNGAADMGPLITAAH
                     RAKVTDYIAAGVAEGARLVVDGRGLKVAGHESGFFLGGSLFDGVTSSMKVYREEIFGP
                     VLVVVRVPTIADAVKLVNAHEYANGTAVFTRDGGAARAFVQGIEVGMVGVNVPIPVPM
                     AFMSFGGWRRSIFSDHAMHGMEGVRFYTRLKTVTSRWPEGKGAAEFVMPTMK"
     gene            10912..11568
                     /locus_tag="VEW08_02725"
     CDS             10912..11568
                     /locus_tag="VEW08_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39675.1"
                     /translation="MEKTPAGHQKLLQDIERLLAEAPDEASRRDLERLRDSLNSPQMI
                     EMAREVMNKPRSPGTLMLDFHDPLLPGAVTAAGGVIAAAFCLYAVTLGFEKPAIAVGD
                     SMINLWIVAAFAGALSACFTALSFSRTFTVRFDTEGMLSRTNGSRWRRLYTGAMSWKS
                     IRSLHERAVDRVLEVRSASGEVFEIPMKVVNYRALRDHLDNMVMLYGEDHGGAGHQKS
                     "
     gene            complement(11634..12524)
                     /locus_tag="VEW08_02730"
     CDS             complement(11634..12524)
                     /locus_tag="VEW08_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ39676.1"
                     /translation="MALAAARTADYARLIAVPAIWGGTFVAGKHVVAVLSPMMGSFSR
                     YVIACAALLVAAFALEGGLPKLTRNQWLATMVLGAFGVFAYNLFFMASLERLPGSRAA
                     LIIALNPVITITISAIVLKEQLSARRWLGVVIALFGVWIVISHGDIASIASAGIGLGE
                     IFMFGAVTSWALYTIIGRKVLGGLTPLAATNYAALWGTLLLACVAAPEFGSITTSQFD
                     WTMIGALLYLGIGGTALAFVWYYVSVKKFGTAVTSIFNNLVPVFGVLISVLLLGEHLL
                     LSMLIGGAIAIVGVMMVSRP"
     gene            complement(12524..13312)
                     /gene="cysQ"
                     /locus_tag="VEW08_02735"
     CDS             complement(12524..13312)
                     /gene="cysQ"
                     /locus_tag="VEW08_02735"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HYJ39677.1"
                     /translation="MSDLLQSLIYTSQRAGGAIMAVYATDFSVRGKADESPVTAADEA
                     AEKIILADLAAIAPGVPVIAEESVAAGSVPAIAERFFLVDPLDGTREFVARRDEFTVN
                     VALIENGEPILGVVFAPARRELYWGDVKAGRAGHIDADPDGTMPSLGNAISARRPPAG
                     GLTVVASRSHRTPETDAFLANYPVAEFRSIGSSLKFCLVAAGEADLYPRIGTTMEWDT
                     AAGHAVLQAAGGRVTGLVGEPFRYGKPGFRNGNFLAQGLARGKP"
     gene            complement(13309..15507)
                     /gene="malQ"
                     /locus_tag="VEW08_02740"
     CDS             complement(13309..15507)
                     /gene="malQ"
                     /locus_tag="VEW08_02740"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HYJ39678.1"
                     /translation="MSSDELTALIERLARARGIGDAYYSYKGELRHFTLATKAAILRA
                     MHCRLDDATALRQQIQESEAAHPVGLSGDVVVLRNGARAARINTPAIEKNALLKWTVQ
                     FEGGGGSRSGEVRAWELPERGSHQEDGNWYVLRDLPLPADLPLGYHRLELDLEFAGRE
                     SCPLIVTPDKCFEPGGLAGSARIWGVAVQLYALRSENNWGIGDFSDLAELLRLASAAG
                     AGFIGISPVHALFPSDPTLFSPYSASSRHALNIMFIDVAAVLEVQGSRRAQAIMADPG
                     FRARLGQVRAATHVDYPAVASLKMTVLRAAFERFRTEHLARHTARAAACRAFQRERGE
                     VMRLHTLFDAIDGHLRRHHGTGAGWHNWPEEYRAPGGEAVKRFAQEHADEVDFHAYLQ
                     WIAAEQLGAVRQLARELNLDVGIYGDYAVGVNASGSETWSDQSLYCIGAAIGAPPDPI
                     GLGGQEWGIPPQDPRQLRRTAYAPFVALVRASMRNCGALRLDHVMAMFRQWWVPRGFK
                     SADGGYVHYPLDDLLGVVALESHRNQCLVVGEDLGVVPDEIRRALPQYGVYHYKVVLF
                     EQKNGEFVAPAGYIRHALAVVTTHDLPTLHGWWSGHDIDLWEKLGVYADASVVDRART
                     ERQAERERLLRALRLEHLWSEESNLVPEYSPEFGRAVHAYLARSPTALVTVQIEDMIG
                     MLEPVNVPGTSSEYSNWTRRVALSAREIFARPDVRELCAAMVASRKMAAP"
     gene            complement(15497..>16416)
                     /locus_tag="VEW08_02745"
     CDS             complement(15497..>16416)
                     /locus_tag="VEW08_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39679.1"
                     /translation="GLGGYQLGNFPAGWSEWNDRYRDTMRAFWHGGRRMLGGYAERFA
                     GSSDLFRRNGRKPTASINFVAAHDGFTLRDTVSYNDKHNEANLENNRDGHSNNHSWNC
                     GAEGPTDDAHIIALRRRQVRNMLTTLFFSQGVPMLLAGDEMYRTQDGNNNAYCQDNEI
                     SWVNWELLKSDDSLLQFVRALAALRRARPELRRDTFLKGALQASHSRDICWWHPAGHE
                     MADQEWNDPDQRCLGVGLGGTAASPDLMLLLNPTEAECDFRLPANPGGHWDVVLDTAH
                     PVGVMSGFQLPTGPVKVGPYQTLALQRVV"
BASE COUNT         2928 a         5468 c         5293 g         2727 t
ORIGIN      
        1 atcaggctct cgacctgcac gtccttcatc tcgagcttca ggcccatctc gcggccgagg
       61 atgatcacct tgcgcacgaa gtccgtgccc gacaggtcgt cgcgcggatc gggctcggtg
      121 taacccttcg ccttcgcgtc cttcacgatg tcggaaaacg gggtcttgcc gtcgtacacg
      181 ttgaatagat aggccagcgt gccggagaaa atgccctcga cgctggtgat cttgtcgccg
      241 gtctcgcgca ggtcgcgcag cgtctggatc accggcaacc cggcgcccac ggtggcttca
      301 tataaataag aggagccgcc gagccggcgc gcctccttga gcgattcgta gtaggccagc
      361 ggcccgctgt tggccttctt gttgggcgtg accacgtgga tgcccgccgt gagccactcc
      421 gcgtagtgct tcgccacgct gtcatcggcg gtgcagtcga tgatgaccgt gtgcggcagg
      481 tggtcgacgt gcaggtggtc gacgaatttg ccgatgtcgg cagcatgcgg gctcgcgtcc
      541 agcgccgccc tccaactatc ttttggacca tcaacggcaa taccctgctc cgccagcagc
      601 attttctttc tgcccatgat gccgcgcagg cgcaggtcga gtttgaaatc gtcacgcagt
      661 cggctgcttt cggcggcgat ctgctcgagc agcgcgcgac cgaccgagcc cggtccgatg
      721 aggccgatgg agatggtgtg cggcgatagg tagaagccgg catgcacggc gcgcagcgcg
      781 cgcgtggcgt ccttgccgtc caccaccacc gagatgttgc gctccgaggc gccctgcgcg
      841 atggcgtgca cgttcactgc cgccgaacca agcgcgttga acacctggcc cgagacaccg
      901 ggaagcccgg ccatgccgtc gccgaccacg gcgaggatcg ccaggtccct gtcgaccgcg
      961 atgctctgga tctggccttc cttgatctcg cgatcgaacg cggctgcgac tacgcgcgcg
     1021 gcgcggtcgg cttcgacacc gggaatggcg cagcagatgg aatgctccga gctgccctgc
     1081 gaaatgagga tcaccgaaat gccctcttca cgcagcgcgc cgaacaggcg gtgagccgtg
     1141 ccgggcacgc cgatcatgcc ggcgccctcc acgttcacca tcgcgatgtt ctcgatgctg
     1201 gtgatgccct tcaccggtag tttggagtcg ggcttggcgc agatcagcga acctggcttc
     1261 tgcggcgcga aggtgttgcg gatccagatg gggatgccgc ggccgatggc tggcgccatc
     1321 gtctgcggat gaatgacctt ggcgccgaag tacgcgagct ccatggcttc gttgtacgac
     1381 agcgagtcga tgaccttggc gtcgggcacg cggcgcggat cggccgacag cacgccatcg
     1441 acgtcggtcc agatatggat ctcgctggca tcgagcagcg atccgaaaat cgaaccggag
     1501 aagtcgctgc cgttgcggcc aagagtggtc tgtacgccgc ggcgatcgga ggcgatgaag
     1561 ccggtgatga tcagagtgcc tttgaatgcc ccgtctttcg cagacgtggc gaccagcgcc
     1621 gaaagcttgg cgcgcgattc ttcccactgc acggcgggtc ccaacggccc ccactcgacg
     1681 accacggcct ggcgcgcgtc gacccacttc acatcgcccg gccgtttcgc acgggccgaa
     1741 aagaattcac gaaagagctt ggtggaccag atttccccgt aaccggcgat caggtcgcgc
     1801 acgttctgcg cggccgagcg cgtgagcttc accgtgtgca ggatgccttc gatgtcgtga
     1861 cggtcgcggt cgaaaccggc catgtagagc tggcgcgcat cgcccgacag cagggcttcg
     1921 gcgatggtgt tgtgccggac gcgaagcgca tcgagttcac cgcgccaggc gtcgtcctgc
     1981 gcttcggcac gagcgaccag cccgagcaac gcatcggtga cacctcggca agccgagaga
     2041 acgattccca ggcgacccgg cggcagcgtt tcgaggatgt tggcgacgcg cttgaaacac
     2101 tcggcgtccg cgaccgaaga accgccaaat ttgtgtacga cccaggcgga ggggtcagca
     2161 gattgcattg aagcctaaac cttgcggtaa aaaagggggc aaaaaaagcc cggcaaatct
     2221 aaaggttgga cccatccacg cgcaagccgg agccactcaa actggcatat atttgcgccc
     2281 cacatgtcgc tgcaccgcct cctcccgcta tacgaccgcg cattgctcgc caatgagccc
     2341 ctggtactcg ccacggtcat caggactggc ggttccacct atgccaaacc cggagcgcag
     2401 atgctcatcg ccgccgacgg cgaatacgcg ggtctgttgt cgggcggttg tctcgaaggc
     2461 gacctgcgcg aacacgcgcg tcatgtcgca gccagcggcc aggcgcgaat cgtgagctat
     2521 gacctgcgca gcaccacgga tcaattgttc gggctgggcg ccggttgtga gggcgccatg
     2581 gacgtcctgc tgtcgcgagt gtcggcggcc gagggctggc aacctctcgt ggccatggcc
     2641 gagagttttc gcgccgcgcg cgaacaacat ttcgccttcg tgattgcaag cacggatccg
     2701 gcgtatccgc tcggcgtttc atttccggta aatgggcagg tcacgccata tcccagtggt
     2761 gtcgaagtat tcgtcgcggc ggtggcgccg ccaccgcacc tgctgctgtt gggcggaggg
     2821 ccagacgccc gccccgtagc cacgctcgcc gccttcctcg gctggcgcat cacggtggtc
     2881 gaacatcggg ccgtctatct cgcgcccgat cgctttccgc cgtcgacgag attgatcgaa
     2941 acgcgtccgg acaacgttgc cgccgcggtg cggttggcgg agttttcggc agcgatagtc
     3001 atgagccatc acctggaatc cgacctgcac tacctgcgtg cccttgcccg ggctccggtg
     3061 ccctatgtcg gcttgttagg gccggccgca cggcgcgaaa aactgctgag cgacctgggc
     3121 gaggacgccg gcacgctgcg cccccggttg cgcgcgcccg tcgggctcga cattggcggg
     3181 cgcacgcccg aatccatcgc gctcgccatc gttggcgaag tgcatgccgc attggcgggt
     3241 cgcgccggcc gtccgttcag cgagacattg aagtccgaca gcaccgcgtc cgccgacggt
     3301 agcgagcggt agcttggaag aactcctgcc tctcatcgcc atcctggcgg tgtccggact
     3361 catcgcggga ttcgctgccg ggctcctggg cgtgggcggc ggcatcgtga ccgtgccggt
     3421 gctcgaatac tcgctgcggt tcgccgacgt gcccgagcag taccgcatgc acgtcgcggt
     3481 ggcgacctcg ctcgcagcca tcattcccac ctcgatcagc tcggcgagca cccaccattc
     3541 gcgcggtgcg gtcgattggg agctggcgcg gcgctgggca gcgcccatgt tcgtggcttc
     3601 cttcgccggc agcctgctgg catcgcgcgc gccgctcgcc gtgctcgccg gcgtgttcgg
     3661 cagcgtcgcg ctgctgatcg cgctcaagat gttgctgccg ttcgatcact ggcgagcccg
     3721 tgaaagcgtg ccgcgcggcg ccgggggcgc ggcactggca tcgctgatcg gcggcgtttc
     3781 ggcggtcatg ggtatcggcg gtggcacgct caccgtgccc acgttgaacc tctgcggata
     3841 tcccattcac cgcgccgtcg gcacggcgtc gttcttcggg atcgtgatca gcattcccgg
     3901 caccgtaggc tatctactgg cgcagtcgcc ggtggcgctg ccttgggcga cggtcggttt
     3961 cgtcagtgtc gtggggttgg cgatcatcac gccgggctcg atgctcacgg cgacgctcgg
     4021 cgcacgcgtc gctcactcac tgagccgccg ccgcctgtcg caggctttcg gcgtgttcct
     4081 gctgctggtg ggctcgcgga tgatctatcg cgcgctggcc tgaacgtttt ttggcgccgt
     4141 gacatcgact tcttcgaaga cgacgtcgat gggaatgagc ggcacggggc agtcgaccac
     4201 catgcagatg ccgcgcagca gatcgacttg cggcacggtg aggcgtccgc ccaccgcaat
     4261 gacgcgcgcc atgccttccg acagcgagcg acggcccgtg ggatgaatct gggcaacggc
     4321 cgtcagcgcg gaatcgagcg ccgcgggcgt gatcggttgt tcgggatacg gcgcccactt
     4381 cgttggcggc aacataccca tgaggcccgc gcgataggcg ttctgaccct gcttgcccgc
     4441 gccgaagcgg cattgcgcca gcgccgcata cagttcgcac accattggcg cttgttcatg
     4501 caatggcacc gggggggcct cctccctggc tttggcgaga cgcttctcga tcattcgtgt
     4561 cagcgagaag cgcagcatgt cgccggtggc caccgtgggt gcgaacgcgc gcgcaatcgc
     4621 gcgcaggcgc ttgcgatcgg cgggctgcat gtcgtcgagc agcgagaaca aatccaccag
     4681 caacggcagg cgcgatgccg gcgcaagctc gccgacgcgc gccacctgcg cctgcgtttc
     4741 cttgaatagt tcgataccga gcagaggcgc gaggcgcgtc aattgcgtgc gccacttcgc
     4801 cggctcagaa gccagcatgg tggctacgaa cgtggcctgg acggcgatgc cggacaactc
     4861 cacgcgtgcg acgaaagtgc cgatcgcagc ctgttgatcc tgtgaaagcc ggttgcgcag
     4921 cacgtccggc ggcaacgcgc gtcccgcgac cattcgcaca ccagtcggta gcgtctcggc
     4981 gagaatcggc ttttcgagcg tcaggtgaat gccgctgtcg gcggccgagg tggtcggcgc
     5041 cggagcggct cgcgcaggtg cgcgagcgcg cgacgcagtc gcgggaacgg gccccgcggc
     5101 catggccgcc acgggtgaga actgcagcgc attcgaagat tccgacgcga catcgccttt
     5161 ggccgtgggc tcagcggcgg gagccgtggc cgccgcggcc gcggcgcgcg tcgcgatcct
     5221 gcgcttctct tcattgacca gagagcggaa cttgagcggc gtgatacgcg catcgatcgc
     5281 gcgcacgcgt tcgtcgagcg gcggatgcgt ggcgaaccag gaagccttga atttggtgat
     5341 ccaggccgga gagctgccgg cgaagaacat gtgcgagacg ccaatggcgt gctcgtgctc
     5401 gagacgcgtg ccggaagcgc tcgcggccat cacgatgaag gcgctctgca gcgcctgcgg
     5461 attgcgtgtg aactgcaccg ccgacgcatc ggcgagatgt tcgcgacggc gcgagaccgc
     5521 tgcctgcaac aggcgcccgg cgaacgcccc cgcgctgccc gccagaaaaa cacccaatgc
     5581 gatgagcttg atgcgcgctt cgctcttgcc accgccgcgc cgattcatga aacgccgcgc
     5641 gaggtcggtg atcacgcaaa ggccgaagat ccacgcggtc agccgcatgt tgatcttcat
     5701 gtcgccgttg aggacgtgac tgaactcgtg gccgatgacg gcctgcagct gatcgcggtc
     5761 gagcgcatcg agcgcgccct gcgtcaccgc cacggcggcc tcgtcgggcg agagtcccgc
     5821 cgcgaatgca ttgatgcctg cctcgtccgg cagcacgaac acctgcggct tgcgaatgcg
     5881 cgccgcgata gccatctctt cgactatgtt cacgaggcgc tgacgcttga ggtcgccatc
     5941 attggcgttg acctgaacgc cgcccagcgc gcgggccacc gcggggccac cttcgcgcat
     6001 ctgcgagctt cgatgccacg tggcgatcag gatggtgccg aagacgacca atgtgatcca
     6061 gaagaccaca tggggttcgt ggcgcagcac ccaccgcggg tcggcgccaa ggccgagaat
     6121 gaccaccagg ttcacgacca gcacgaccag caggatcgca gcgacaaatc cgctgaccag
     6181 ccagagcgtg tacttgcgct gggcatcctg acgttcgaaa aactgcgtgg tcacatctct
     6241 aacccgttga ctggaaggct gcaaattaac gccaatttga taggtagggc gtgcactggc
     6301 gcacgacccc gccgcaaaaa aacccgccaa atgcgacccg tttaacggtt gcggtcaatg
     6361 gggcggcagc aaggcggaac cggagtaata tcgcccggca tgaacctgga accgcagaag
     6421 tccgagtccg aaatccgcaa ctacagcggc gtggcccgga tactcgccag tgtcaccata
     6481 ctggccctgg ccgccacgac gatcctcgtc gtgctggaaa taattccgcg ctcaacgttt
     6541 gccgaaatgg ctggcaagat cgcgatggtc gccggcgtct gcgtggtctc cgccctggcc
     6601 atcgggttcc tctcccgacg gtgaacgaaa gcgggagcac cgagtcgagc cacatccacg
     6661 tcgtggtgct ggctgcgggc aactcttcgc gattcggatc tcccaaacaa ctggtgcgcg
     6721 tcaacggacg cccgctcatg cacaccgtcg tgagtcgcgc ggtggaactt gccggtcatt
     6781 ccgtcaccgt cgttctcggc gcgaatgccg ctgaacttgc gccattgctt cgccacagcc
     6841 cggcgggcat cgcggtgaat cgcgactggg ccgaaggcat tgcgagttcg atccgcgaag
     6901 ggattgcgca cgcgccgcaa gcggccgacg gtgtactcat cgcgctcgcc gaccaggtgg
     6961 ccgtgaccac tgaagacctg cgacgtctcg ccggagtatg gcggcgtact cctgcatcca
     7021 tcgccgccgc tcagtacgcg ggcgccgtcg gcgtgccggc gatttttccc cgctggtgtt
     7081 ttcgcgaact gaacgagctc cgcggcgatc acggcgcgca gctgctgctg atccggcatg
     7141 tcgaccggtt gatccgtctg cccatgccga gcgcggagct cgacatcgat cggcccgaag
     7201 acctgctggc gctcgaagcg aacacggaac tcaaacgcgg agaaacaccg gaagccgaat
     7261 agcccggtgt cgggggacgg cgcctattgc cccactattc gcgcgagcat tctcgccagc
     7321 ggaaacgcca gcacgacgaa cgtgcacggg acgatggtca gcaccagcgc cgcccacagg
     7381 tgaagtggag cggcgcgagc gaggcgttct gcccgcgcaa accgctgcgc cgccgactga
     7441 cgcgcccgtt cgcggaccac ctgcgcggcg gggatgccga atttttccgc cgagcgcagc
     7501 gccgtcacga gtatggccag aggcttgaga cccgtacgtt gctccattgc gcgcaatgct
     7561 tcgagcggct ccgcaccgcc atgaatgtcg aggacgacgc gcatccaggc gcgcctcagt
     7621 gcgccgtctg gcgcacgatg cgcgcaggtt gcaagcgccg cgggaagcgc ccagcccgcc
     7681 tccattgcca gcgcgatgag atcgagatgc acgggcagat cgtcgagcaa ttcgtgctcc
     7741 acgcgctgcc gcgccgaggc gagaaaccgc ggcgccaccc agtagccgag tccggcgact
     7801 gccgcggccg ccagccatgc gccgttagca aggccggtga agaacaccgg cacggcggcg
     7861 gccagaccca accacgcgcg cgtgacctgc caatgcgcag gcgaagccag ccgattgagg
     7921 tcggcggatc tcaatgcctc gcgaatgtcc atcaatccgc gcaagccgaa cacgctcagg
     7981 ccgaacatga cggcagcgag aatgaccaac gcgaagagtg gcattcagat tgccacacgc
     8041 gccatgcgcc acagcagcca gcaaccgcag gcttcgagcg caacgacgaa cagcagcgcc
     8101 gcgagcggca cctggatgcc tgccgcgtca agcgcaagca gcgccagcgg cggaacggac
     8161 gcggccacgg cgccacgtcg ccggcctgat gccgtgcgcg cgagcatctt gcgttcgaag
     8221 gccacgcgcc gtttcaggcg cagtgcaagc cgctgcgcgg tatctgccag cagctgcggt
     8281 gtcgggtgcg gaagttcctg caccagcaac agcgaaacga actccggcgc gcgcaggcgc
     8341 tgcacgcgct tgcacaacgc ggagtccacc ggcagacacg ccccactctt gcgaaccgcc
     8401 acgcccaacg cgcccagcgc gcatgcgaca tcgctggctt cgcacatggc gcgccgtcgg
     8461 ctggtggccg cgagcgcggg caatgcgatg aacgcggcga acaccaccat ggagccaggc
     8521 aggaccccgt caaatggcat cgccgactcc gccggtcagc aaccacctgc cgtaaccctg
     8581 ccacaacccg atggcgagca cagcggcgag ccatgccacg acgaggcgag tgagcggccg
     8641 cgaaacgtgc cttgccctga ccttgagttg ctgcgcatcc atgcgcgttc tccgaccgga
     8701 aggtgcggag tattggagcg cgaatcccca tcggcgtcag cgcggcaatc gcctgcgaac
     8761 ggccaggttt gcgacttgcg cgaacccggg gaagtcaggc cttggtcacc gaaacccgct
     8821 ttgccgtgat ggcatcctgg ttggccgcca cgaatgcatt gagatagtcc ggattggtga
     8881 atgccagctt catgaagccc acctgtcctt caccgttcat gagccccgtg aattgcaaac
     8941 gcacgttctt gatgcgcacc ggttggtaga agctggtctg gggcgccttc ggccggcgca
     9001 gccggtagaa caggatcgtc aacaccacgc ccagcaccac gctgcccgcg tacatgtaat
     9061 cgatccacag cggcaacgcg actgttgaac cggcagcaag gcagattccg aaaatcacgc
     9121 cggtgatcag cagcagcacg ccgagacgcg aaaagaaacc cgctggcttg cgccggatcg
     9181 tcctctggca tcccgcgcag atcggcacgg atagtttgaa ggtcgtgtcc gtgcggccga
     9241 tgccgaaaaa gcgcgtgatg cgtgtgtcct ggatctcgct cttgcactcg gtagcgccac
     9301 agttgcagca aaacgaagtt ggaaaaccaa gctgcggatt ctcgacctgc attgatcgac
     9361 ctctttgttc ttatttcatc gtcggcatga cgaactccgc agcacccttg ccttcgggcc
     9421 agcggctggt aacggtcttc aggcgcgtat agaaacgcac gccttccatg ccgtgcatgg
     9481 cgtggtccga gaagatcgac cggcgccagc cgccgaaact catgaaggcc atgggcacgg
     9541 gaatgggcac gttcacgccg accattccca cttcgatgcc ttgcacgaag gctcgcgcgg
     9601 cgccgccgtc gcgcgtaaac acggcggtgc cgttggcata ctcgtgcgcg ttcaccagct
     9661 tgactgcgtc ggcgatggtg gggactcgca ccacgaccag cacgggaccg aaaatttctt
     9721 cgcgataaac cttcatcgac gaagtcacgc catcgaacag gctgccgccc aggaaaaaac
     9781 cgctttcgtg gcctgccacc ttgagcccgc ggccgtccac gaccaggcgc gcgccttccg
     9841 cgacgcccgc ggcgatgtag tccgtgacct tggcgcgatg cgcggccgtg atcagcggac
     9901 ccatgtcggc cgcgccgttc tgcgactgat ccacacgcag gccgcgcact cgctctgcga
     9961 gcgcgcccac caggcggtcg gcggtggcat cccccacggc gaccgcgacg gagatcgcca
    10021 tgcagcgctc gcccgccgaa ccgtaggccg cgcctaacaa ggcgtcgcag acctgggtcg
    10081 tgtccgcatc gggcaacatc accaggtgat tcttcgcgcc gcccagcgcc tgcactcgtt
    10141 tgccgtgcgc cactccttca cgctgcacgt attcggctat aggagtcgag cccacaaagc
    10201 tgaccgcatt cacgtccggg tgacgcaaca atgcgtcgac agaaactttg tcgccattga
    10261 ccacgttgaa cacgccgtcc ggcagaccgg cttccttcaa ccattgtgct aacaacagcc
    10321 cggggccggg atcgcgctcg gacggcttga ggatgaagca gttgccgcag gccagtgcca
    10381 cggggaacat ccacagcggc accatggcgg gaaaattgaa cggcgtgatg ccggccgcta
    10441 caccgagcgg ctgccgcagc gaataactgt cgacgccggt gcccacgttg tcggcgaact
    10501 cgcccttcag caggtgcggt gcaccgcagg cgaattccac cacttcgagt ccgcgcgtca
    10561 cctcgccggc cgcatcgctc accagcttgc cgtgctccga ggcgatcagc gccgcgagcg
    10621 cgtcgcgccg ctgttcgatg atctccttga atttgaacaa cacgcgcgca cgcttgagcg
    10681 cgggagtggc ggcccaggcg ggaaaggcag ctcgcgccgc gcgcaccgcg gcatcgacat
    10741 ccgcttcatt ggccagcgcc actcgcgcgg ccacgactcc gagcgacgga ttgaatacgt
    10801 cgccggcgcg gccggaagtg ccggcgacca gctggccacc gatccagtgt gagtaactgc
    10861 ttacgcgctc gtgtgtcatg ggccgcgagt gtagcgctct ataatgcagg catggaaaag
    10921 acgcccgccg gacaccagaa attgctgcag gacatcgagc gcctgctcgc cgaagccccc
    10981 gacgaagcca gccgccgcga cctcgagcgg ctgcgggatt ccctgaattc gccgcagatg
    11041 atcgagatgg cgcgcgaggt catgaacaag ccccgctcgc ccggcacatt gatgctcgat
    11101 tttcacgacc cgctgctgcc gggcgcggtg accgcggccg gcggcgtcat tgccgccgcg
    11161 ttctgcctct atgccgtgac gctcggcttc gaaaagccgg cgatcgcggt gggcgacagc
    11221 atgatcaacc tgtggatcgt cgcggctttc gccggcgccc tgtcggcctg tttcaccgca
    11281 ctgtcgttct cgcgcacgtt caccgtgcgt ttcgacaccg agggcatgtt gtcgcgcacc
    11341 aacggctcac gctggcggcg cctgtacacc ggggccatga gctggaagag catccggtcg
    11401 ctgcacgagc gcgctgtcga tcgggttctc gaggtgcgtt ccgccagtgg cgaagtgttc
    11461 gagatcccga tgaaggtggt gaactatcgg gcgcttcggg atcatctcga caacatggtg
    11521 atgttgtacg gagaagacca tggtggtgcc gggcaccaaa agtcgtagtt gagccagcgc
    11581 tcgcgtttct gccaacgttc gctcaactac gacttttggt gcccggcacc gcctcagggc
    11641 cgcgacacca tcatcactcc gacgatcgca atcgctccgc cgatcagcat tgacaggagc
    11701 aggtgctcgc ccagcaggag cacgctgatc aggacgccga ataccggcac caggttgttg
    11761 aagatgctgg tgactgccgt gccgaacttc ttcaccgaca cgtagtacca gacgaaggcc
    11821 agcgcggtgc cgccgatgcc gagatacagc aacgcgccga tcatggtcca gtcgaactgg
    11881 ctggtcgtga tgcttccgaa ctccggcgcc gccacgcagg ccagcaacag cgtcccccac
    11941 agcgccgcat agttagtcgc cgcgagcggc gtcaggccgc cgagcacctt gcggccaatg
    12001 atggtgtaca gcgcccatga cgtgacggcg ccgaacatga agatttcgcc cagaccgatg
    12061 cctgccgaag cgatgctggc gatgtcgccg tgcgaaatga cgatccacac cccgaacagc
    12121 gcgatgacaa cgcccagcca gcgccgcgcg ctcagctgtt ccttgagcac gatggccgag
    12181 atggtgatgg tgatgaccgg attgagcgcg atgatgagcg cagcgcggct gcccggcagc
    12241 cgctcgagag aggccatgaa gaacaggttg taggcgaaca cgccaaacgc cccgagcacc
    12301 attgtcgcga gccactggtt gcgggtcagc ttcggcaagc cgccttccag cgcgaaggcc
    12361 gctaccagca gcgcggcgca cgcgatcacg tatctcgaga acgagcccat catgggcgac
    12421 aacacggcga ccacgtgttt gcccgcgacg aaggtgccac cccagatggc gggcacggcg
    12481 atgagccgtg cgtagtcggc ggtgcgcgct gcggccaatg ccactacggc tttcctcgcg
    12541 ccaggccttg cgccaggaaa ttgccgttgc gaaatcccgg cttgccataa cgaaacggct
    12601 cgccgacgag gccggtgaca cggccgcccg cggcttgcag cacggcatgt ccggcggcgg
    12661 tgtcccattc catggtggtg ccgatgcgcg gatagaggtc ggcctcgccg gccgccacca
    12721 ggcagaactt cagcgacgag ccaatggaac ggaactcggc caccgggtag ttagcgagga
    12781 aggcgtcggt ctcgggcgtg cgatgcgaac gcgaggcgac tacggtcaga ccgcctgcgg
    12841 gaggccggcg cgcgctgatg gcgttgccca acgagggcat ggtgccgtcc ggatccgcat
    12901 cgatgtggcc cgccctgccg gccttcacgt caccccagta caactcgcgg cgcgccgggg
    12961 cgaacaccac cccgagaatc ggctcgccat tctcgatgag cgcgacgttg acggtgaatt
    13021 catcgcgacg ggccacgaac tcgcgcgtgc catccagcgg atcgacgagg aagaaacgct
    13081 cggcgatcgc cggcacactt cccgcggcca ccgactcttc ggcgatgacc ggcacacccg
    13141 gtgcgattgc cgcgagatcc gcgaggatga ttttttcggc cgcctcgtcg gcggcggtga
    13201 ccggcgactc atccgccttg ccgcgcacgg aaaaatccgt ggcgtagacg gccatgatgg
    13261 cgcctccggc acgctgggag gtgtagataa gcgattgcag caggtcactc atggtgccgc
    13321 catcttacgg ctcgcgacca tggcggcgca caactcgcgc acatcgggcc gcgcgaagat
    13381 ttcgcgcgca ctcagcgcca cgcggcgggt ccagttggaa tattcgctgc tggttccggg
    13441 cacattcacc ggttcgagca tgccgatcat gtcttcgatc tgcacggtga ccagcgccgt
    13501 gggactcctg gcgaggtacg cgtgcacggc tcggccgaac tcgggggaat attccggcac
    13561 caggttcgac tcttcggacc agagatgttc gagccgcagc gcgcgcagga gccgttcacg
    13621 ttccgcctgg cgctccgtac gcgcgcgatc gacgaccgaa gcatcggcgt agacgccgag
    13681 cttttcccac aagtcgatgt catgaccgct ccaccagccg tgcaaggtcg gcagatcgtg
    13741 cgtcgtgacg accgccagcg catgccggat gtaacccgcg ggcgcgacga actcgccgtt
    13801 cttctgctcg aacaggacga ccttgtagtg atagacgccg tactgtggca gcgcgcggcg
    13861 gatctcgtcc ggcaccacgc ccaggtcctc acccacgacc aggcactggt tgcgatggct
    13921 ctcgagcgcc accacgccga gcaggtcatc gagcggatag tggacgtagc cgccgtccgc
    13981 ggacttgaaa ccgcgtggta cccaccactg gcgaaacatc gccatcacgt gatcgaggcg
    14041 cagcgcgccg caattgcgca tgctggcccg caccagcgcc acgaagggcg catacgcggt
    14101 tcggcgcagc tggcgcggat cctgcggcgg aatgccccac tcctgtccgc ccagtccaat
    14161 cggatccggt ggtgcgccga ttgcggcgcc gatgcagtac agcgattggt cggaccaggt
    14221 ctcggaaccc gacgcgttca cgccaacggc gtagtcgccg tagatgccga cgtcgagatt
    14281 gagctcgcgc gccagctggc gcaccgcgcc taactgctcg gccgcgatcc actgcaggta
    14341 ggcatggaag tccacctcgt cggcatgttc ctgggcaaag cgctttaccg cctcaccgcc
    14401 gggtgcgcga tattcctcgg gccagttgtg ccagccggcc ccggtgccgt gatgacggcg
    14461 caaatgaccg tcgatcgcgt cgaacagagt gtgcagccgc atcacttcgc cgcgctcacg
    14521 ctggaatgcg cggcaggccg cggcacgcgc cgtgtggcgc gcaagatgtt cggtgcggaa
    14581 tcgctcgaag gccgcgcgca gcacggtcat cttgaggctg gcgaccgccg ggtaatcgac
    14641 gtgtgtggcg gcgcgtacct ggccgagccg cgcgcgaaag cctggatccg ccatgatggc
    14701 ctgcgcacga cgcgagccct gcacttcgag caccgcggca acgtcgatga acatgatgtt
    14761 caacgcgtgc cggctggagg ccgagtacgg cgaaaacagc gtgggatccg acgggaacag
    14821 cgcgtgcacg gggctgatgc cgataaagcc cgcgccggcc gcggatgcca ggcgcagcaa
    14881 ctccgcgaga tcggagaaat ccccgatgcc ccagttgttt tccgaacgca gcgcatagag
    14941 ctgcacggcc acgccccaga ttctggcgct gcccgcgagc ccccctggct cgaaacactt
    15001 gtcgggcgtg acgatgagcg ggcaactctc cctgcccgcg aattcgaggt ccagctcgag
    15061 ccggtgataa cccagcggca ggtccgcggg taacggcagg tcacgcaaca cgtaccaatt
    15121 gccgtcttcc tggtgagaac cgcgctcggg caattcccag gcgcgcacct cgccactacg
    15181 cgaaccacca ccgccctcga actgtacggt ccacttcagc aacgcgttct tctcgatcgc
    15241 cggtgtgttg atgcgcgcgg cgcgagcgcc gttacgcaac accaccacat cgcctgacaa
    15301 acccaccggg tgggccgctt cggactcctg aatctgttgc cgaagtgccg tggcatcgtc
    15361 gagtcggcaa tgcatggcgc gcaggatcgc cgccttggtg gccagcgtga aatgcctgag
    15421 ctcgcccttg taagagtaat acgcgtcgcc gatgccgcgc gcgcgggcca gtcgctcgat
    15481 gagagccgtc aattcgtcag acgacacgct gcaacgccag ggtttgatac gggcccactt
    15541 tcacagggcc ggtgggcaac tgaaacccgg acatcacgcc gaccggatgc gcagtatcga
    15601 ggaccacatc ccagtggccg ccgggattcg cgggtaaacg gaagtcgcac tccgcttccg
    15661 tggggttgag taacaacatc aggtctggag aggccgccgt gccgccgagc cccacgccga
    15721 ggcaacgctg gtccgggtcg ttccattcct ggtctgccat ctcgtggccc gcaggatgcc
    15781 accagcagat gtcgcgcgag tgactggcct gcagcgcgcc tttcaggaaa gtatcgcgcc
    15841 gcagctcggg gcgcgcgcgc cgcagcgccg cgagtgcccg gacgaactgc agcagtgaat
    15901 cgtcggactt gagcagctcc cagttcaccc agctgatctc attgtcctgg cagtacgcgt
    15961 tgttgttgcc gtcctgcgtg cgatacatct catcgcctgc cagcaacatc ggcacgccct
    16021 gcgagaagaa cagcgtggtc agcatgttgc ggacctgccg gcggcgcagc gcgatgatat
    16081 gtgcgtcatc ggtgggacct tcggcgccgc agttccagct gtgattgttg gagtggccgt
    16141 cgcggttgtt ctcgaggttc gcctcgttgt gcttgtcgtt gtacgacacc gtatcgcgca
    16201 gcgtgaatcc gtcatgcgcg gcgacgaaat tgatgctggc ggtcggtttg cggccgttgc
    16261 gccggaacag gtctgaagag cccgcgaagc gttccgcgta tccgcccagc atgcggcggc
    16321 cgccatgcca gaaggcgcgc atagtgtcgc gatagcgatc attccattcg ctccacccgg
    16381 ccggaaaatt tccgagctga tagccgccca acccca
//
LOCUS       DASQJS010000071         3598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3184466, whole genome shotgun sequence.
ACCESSION   DASQJS010000071 DASQJS010000000
VERSION     DASQJS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3184466"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VEW08_02750"
     CDS             complement(<1..378)
                     /locus_tag="VEW08_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ39680.1"
                     /translation="MGYEPSDARYQSMKYNRVGRSGVRLPAISLGLWHNFGDVDVFQN
                     GRTIARRAFDLGITHFDLANNYGPSPGSAEETFGALMRKDFHPYRDEMIISSKAGYLM
                     WPGPYGEWGSRKYLVASCDQSLAR"
     gene            complement(396..2168)
                     /locus_tag="VEW08_02755"
     CDS             complement(396..2168)
                     /locus_tag="VEW08_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HYJ39681.1"
                     /translation="MRAHKLSPNILASALLAAVLLPAAIVYSHPDAMGTRFVAGSGTD
                     TGDCDDNHHPCRTLAYALTQVEPGNAIKLAAGSYDVSNIDVENLLIGKEGVRGGYSAE
                     DHFAIQNAETNPTRISGVADEFRNNFIAHGFIVVDANGNALPRIIMPKIAVPTACSGG
                     RAGSFPCHNIDYLAQVQLQEIPGAPTSASEVWGLVDLDDGREYAILGHRNGTAFYDVT
                     TPGAPVLVGNIAGNPSLWREVKAYQFFDASLGRHRAYAYATTEAPGGGLQIFDLTNLP
                     NSVTLANTLTQFSTSHTLYISNINYATNAPLPGATPYLFIAGANIAGGAFRIYDLANP
                     TSPTLVTSPPAGTGYMHDSTSMLITDNRTTQCANAHNPCQVLVDFNETSVDLWDVTEK
                     AAPVRLSTTSYPTASYVHSGWPTADNMAIVVHDELDELQRGLNTHIYTLEIADLRVPN
                     LVTSYIGGTTSTDHNGYTIGNRYYVSHYKRGLVIFDVTNPRALTEIGSFDTYLSPSAN
                     AAGTDGAWGVYPFLTSGTLLVSDIENGLFLLKKNETLPPPPVATNPPPSSGGGGGGGG
                     ALDGSVLILLAGFAMLRARRQTPA"
     gene            complement(2235..2675)
                     /locus_tag="VEW08_02760"
     CDS             complement(2235..2675)
                     /locus_tag="VEW08_02760"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ39682.1"
                     /translation="MRSLAYLLGIVSGFGLTLQIGMNSRLRTVLQSANTAALVSFLVG
                     TAALIGLLIATRAPLPDRDTLASVPWWAWFGGLMGAFYVAISTVVASQLGTASLLGLA
                     LAGQLAMSLAADHFGWMGLPVHPITLTRLAGVALLGAGVWLISR"
     gene            complement(2679..2870)
                     /locus_tag="VEW08_02765"
     CDS             complement(2679..2870)
                     /locus_tag="VEW08_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39683.1"
                     /translation="MPEPVLPVDAATGDARHRDVRAEVFAVVPQHRATFGTRAFWRII
                     LFVAQFPAGLRLLKRLRGS"
     gene            complement(2895..3482)
                     /locus_tag="VEW08_02770"
     CDS             complement(2895..3482)
                     /locus_tag="VEW08_02770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ39684.1"
                     /translation="MSTQFDQAYYKRFYFDSRTAVTSRAEMKARANLIAAYANHIGLP
                     VRRMLDAGCGIGLLRTPLRRAFPRAEYTGLEYSEYLCERFGWTQGSLADWSADPFDLT
                     VCYDVLQYLDDRTAARAIANLGRLTRGVLYLSALTTRDWRENCDRTRTDRDVFLREAA
                     WYSQRLKKDFKPSGVGFWVRRGAPLTTWEMETAAI"
BASE COUNT          679 a         1106 c         1189 g          624 t
ORIGIN      
        1 gcgcgccagc gactggtcgc aactcgccac gaggtatttg cgcgaacccc actcgccgta
       61 cgggcctggc cacatgagat agccggcctt cgaggagatg atcatctcgt cgcgatacgg
      121 gtggaaatcc ttgcgcatga gcgcgccgaa ggtttcttcc gccgagccgg gcgacgggcc
      181 gtagttgtta gccaggtcga aatgcgtgat gccgagatcg aacgcacggc gcgcgatggt
      241 gcggccgttc tggaacacgt cgacgtcgcc gaagttgtgc cacagaccga gcgagatcgc
      301 aggcaggcgc acgccgctgc gtcccacgcg gttgtacttc atcgactggt aacgcgcgtc
      361 ggacggctca tatcccatgg gacggtctct ctatcttatg caggggtttg tcggcgggcg
      421 cgtaacatcg cgaaccccgc caacaaaatc aagaccgagc catcgagagc accaccgcca
      481 ccaccaccac cacccgaaga tggcggcggg ttggtggcca ccggcggcgg cggcagggtc
      541 tcgttcttct tcaggaggaa cagaccgttt tcgatgtcgc tgaccagcaa ggtgccggac
      601 gtcaggaacg ggtagacgcc ccacgcgcca tcggtgcccg cggcattggc cgagggcgac
      661 agatacgtgt cgaatgaacc aatctcggtg agcgcgcgcg gattggtgac gtcgaagatc
      721 acgaggccac gcttgtagtg cgatacgtag tagcggttgc cgatcgtgta cccgttgtga
      781 tccgtggacg tcgtgccgcc gatgtatgac gtgaccaggt tcggcacacg caggtcggcg
      841 atttccagcg tgtagatatg cgtgttgagc ccgcgttgca attcgtcgag ctcgtcatgc
      901 acgacgatgg ccatgttgtc cgcggtgggc catcccgaat gcacgtaact cgcggtggga
      961 taagaggtgg tactcagccg caccggcgcc gctttttccg tgacgtccca gaggtcgact
     1021 gaagtctcgt tgaaatccac gagcacctgg caggggttgt gcgcgttcgc gcactgcgtg
     1081 gtgcggttgt cggtgatgag catcgacgtg gagtcgtgca tgtagccggt gcccgcgggc
     1141 ggtgaagtga ccagcgtggg cgacgtcgga ttcgccaggt cgtagatacg gaacgcgcca
     1201 ccggcgatgt tcgcacccgc gatgaataga tagggagtgg cacccggcaa cggcgcattg
     1261 gtggcgtagt tgatgttgga gatgtacagc gtgtgcgagg tgctgaactg cgtcagcgtg
     1321 ttggcgagcg tgacgctgtt cggcagattg gtgaggtcga aaatctgcag gccgccgccc
     1381 ggcgcctcgg tggtggcgta cgcataggcg cggtgccgac cgaggctggc atcgaagaac
     1441 tggtaagcct tcacttcgcg ccacagcgaa ggattgcccg caatattgcc caccaggacc
     1501 ggcgcgcccg gcgtggtcac gtcataaaac gcagtgccat tgcgatggcc caggatggcg
     1561 tactcgcggc cgtcgtccag atcgacgagt ccccagactt cgctcgcgct cgtgggcgcg
     1621 ccgggaattt cctgcagctg cacctgcgcc aggtagtcga tgttgtggca gggaaagctt
     1681 ccggcccgcc cgccgctgca cgcggtcggc actgcgatct tcggcatgat gatgcgcggc
     1741 agtgcattgc cattggcatc caccacgatg aaaccgtgcg cgatgaagtt gttgcgaaac
     1801 tcgtcggcca cgcccgagat gcgcgtggga ttggtttcgg cgttctggat cgcgaagtgg
     1861 tcttcggcgg aataaccacc gcgcacgcct tccttgccga tcaacagatt ttcgacatcg
     1921 atgttggaca catcgtagct gccggccgcg agcttgatcg cgttgcccgg ttccacctgg
     1981 gtcaacgcat aggccagcgt gcggcaagga tgatggttgt cgtcgcaatc gccagtgtca
     2041 gtaccgctgc cggcgacgaa gcgtgtgccc atcgcgtccg ggtggctata cacaatggcg
     2101 gcgggcaaca gcactgcagc cagcagtgcc gaagccagga tattagggga tagtttgtga
     2161 gctctcattg gccctccatg cgtgccattc cgggcgtagg ttagtgcccg caagcgctga
     2221 taagttctcg attttcagcg cgaaatcagc cagacgcccg cgcccagcaa ggctacgccc
     2281 gccaggcgag taagggtaat cggatgaacc ggcagaccca tccagccgaa gtgatcggcg
     2341 gccaatgaca tggccaattg gcccgccaac gcgagcccta gcaggctggc cgtaccgagc
     2401 tgcgatgcca ccaccgtcga gatggcgaca taaaacgcgc ccatgagacc accgaaccag
     2461 gcccaccacg gcaccgatgc cagcgtgtcg cggtccggca aaggggcgcg tgtggcgatg
     2521 agcagcccga tgagtgcagc cgtaccgacg agaaaactca cgagcgccgc cgtgttggcg
     2581 ctctgcagca ccgtgcgcag ccgcgagttc atgccgatct gcaatgtgag cccgaagccg
     2641 gagacgatac cgagcagata ggcgagcgag cgcatgtact aactacctcg aaggcgtttc
     2701 aggagccgca acccggccgg gaactgcgcg acgaacagga tgatgcgcca gaacgcgcgt
     2761 gtgccgaacg tcgcgcggtg ctgtggcact acggcgaaca cctcggcgcg tacgtcgcga
     2821 tgacgcgcgt cgccggtggc ggcatcgacg ggcagaacgg gttcaggcat ggcaggcagg
     2881 ataactcgcg cgcgtcagat tgcggcggtt tccatttccc aggtggtgag cggcgcgccc
     2941 cgcctcaccc agaatcccac cccgctgggc ttgaagtctt ttttcagccg ctgcgaatac
     3001 cacgcggctt cccgcaggaa tacgtcccgg tcggtacgcg tgcggtcgca gttctcgcgc
     3061 cagtccctgg tcgtcaacgc cgagagatag aggaccccgc gcgtgagccg cccgaggttg
     3121 gcgatggcgc gcgccgccgt ccgatcgtcg aggtactgca gcacgtcgta acacaccgtg
     3181 agatcgaacg gatccgcgga ccagtccgcc agcgatccct gcgtccagcc gaagcgctcg
     3241 cagagatatt cgctgtattc gaggcccgtg tactccgcgc gtggaaatgc gcgcctgagg
     3301 ggcgtgcgca gcagcccaat gccgcaaccc gcgtctaaca tgcggcgcac gggcagaccg
     3361 atgtggttgg catacgccgc gatgagattc gcgcgcgcct tcatctctgc gcgcgaggtc
     3421 accgccgtgc gcgagtcgaa atagaagcgc ttgtaatagg cctggtcgaa ctgcgtgctc
     3481 atgcgcggga cctggccagc tcgcggcgca ccgagcggcg cacgagcaat cccccggcta
     3541 gcccgaccag gccgaccacc accgcgatgc agggacctaa ccagttgtcc atgaacag
//
LOCUS       DASQJS010000072        18432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2553514, whole genome shotgun sequence.
ACCESSION   DASQJS010000072 DASQJS010000000
VERSION     DASQJS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18432
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2553514"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(354..827)
                     /locus_tag="VEW08_02775"
     CDS             complement(354..827)
                     /locus_tag="VEW08_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003117192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HYJ39685.1"
                     /translation="MSAQKLDKVDAKIMQVLSKDGRISWRDLADQIGLSLTPTLRRIR
                     RLESEGVITGYSAQFDEQKLGYGITMYVWVSLERQVEETLKVFERRIADVPEVMSCYL
                     MTGEADYVLRIVVPDLPSYQRFMLDVLTRIPGVSRIQSGLAIKPVFQRAAPPIPR"
     gene            890..1852
                     /gene="metF"
                     /locus_tag="VEW08_02780"
     CDS             890..1852
                     /gene="metF"
                     /locus_tag="VEW08_02780"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF006950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HYJ39686.1"
                     /translation="MALTRSGSYGKLAADSRKQLSLIRNFMSRKPPVSVSFEFFPPAD
                     EAMEKTLWESIRYLEPLHPRFVSVTYGADGSTRERTHNVVSRVQKETDLTCVPHLTCI
                     GASRGEVLDIARKYWDQGVRHIVALRGDAPAGTVKYEPRADGFAYAADLVAGLKTVGD
                     FEISVAAYPEKHPEAPSAEFDLDVLKRKIDAGATRAITQFFYDTDLFLHFRDACAAKG
                     IDSPLVPGILPITRFPQVTRFAKRCGASIPEWLEHRFDGLEENPETRKLISAAVAIEQ
                     VHTLQRHGVDQFHFYTLNRSELTYAICHALGVRPKAASRKAANE"
     gene            1845..5576
                     /gene="metH"
                     /locus_tag="VEW08_02785"
     CDS             1845..5576
                     /gene="metH"
                     /locus_tag="VEW08_02785"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HYJ39687.1"
                     /translation="MSKPVSEDQVKRAQALRQLFAERIVILDGAMGTMIQQHKLDEAA
                     FRGERFKDWPHDLKGCNDLLVLTKPELIADIHGQFLEAGADIVATNTFNATSLSLADY
                     HLEELVYELNFEAVKLARRVADAESKKLGRTCYVAGGLGPTSKTASISPDVNDPGYRA
                     VTFDQLVATYSDATRGLIDGGADAILIETIFDTLNAKAAIYAVRDTLDAAGLDLPIII
                     SGTITDASGRTLSGQTVEAFWASVRHARPITISLNCALGVKQLRPYVEELSRLADTHV
                     GVYPNAGLPNAFGEYDEAPEDTAKVLGELAHAGHLNFVGGCCGTTPAHIKAIADRMRC
                     EPVRKKKDHVPRLELSGLEAFNLTDNIRFVNVGERTNVTGSARFRKLIEANDYPAALS
                     IARQQVQDGAQIIDVNMDEGMLDSQSAMVKFLNLIAAEPDIARVPVMIDSSKWSVIEA
                     GLKCIQGKGVVNSISLKEGEEAFLAHARKVQAYGAAVVVMAFDEQGQADTVERKVGIC
                     ERSYKLLTEKLDFPPEDIIFDPNIFAIATGIEEHNGYGVAFIDATREIRKRCPHAHIS
                     GGVSNVSFSFRGNEPVRQAMHSVFLYHAIQAGMDFGIVNAGQLAVYDDIPKDLRERVE
                     DVVLNRRPDASERLLEIAVNYKDGGARERKEDLAWRQLPIRERLSHALVSGNDQFIIE
                     DTEEARLAAEHPLHVIEGALMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAHLIPF
                     IEALADGVQKTNGTVVLATVKGDVHDIGKNIVGVVLQCNNFAIVDLGVMVPADKILDT
                     AQKLGADMIGLSGLITPSLEEMSYIAGEMQRRGFTVPLLIGGATTSPAHTAVKIDPNY
                     EGPVVYVKDASRAVGVCRQLVTPEMKPAYVAEVKADNVRRREQHAGKKQKGAQLTLGQ
                     ARNNRPKIDWARYTPPVPRFLGAKVFNDVPLAALTPYIDWMPFFNAWEYGGKFPDVLQ
                     DPVRGESARSLYKDAQEMLATILREHWLTARAVVGFFPCNAVDEEIEVYSDTTRSRVL
                     HRLHHLRQQKAKPAGQPHYALSDFIAPKDSGKVDYIGAFAVTAGIGIEPHLERFKAAH
                     DDYNDILLKSLADRLAEASAEALHLRVRRELWGYAPEERLTNDQLIQEAYRGIRPAPG
                     YPACPDHTEKGTLWQMLDVERAIDLRITESFAMYPTAAVSGWYFAHPDARYFAVGPID
                     LDQMQNYATRKGWTAPEARKWLISNLENRISADPHDEAA"
     gene            5653..7314
                     /locus_tag="VEW08_02790"
     CDS             5653..7314
                     /locus_tag="VEW08_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HYJ39688.1"
                     /translation="MTNPEGDNAADQRLTFGRFVLDLRRGVLLLDGREVVLRPKTFAV
                     LSHLVQHPDRLVSKEELLAAVWPNLVVTDDTLVQSIGELRRALGETGARLIVTVPRRG
                     YRFEVSEAPPDRRKARAWHPLRFRWNYGILAPLAVALTVLVMWLAGFWSDAPPAAAEA
                     KPAIAVLPFQHHGEDPAREYLADGLTQDLINSLGRFPALTVMSWNAVAGYKGEAVRPG
                     EIARSLEVRYQVEGSVSYAGDQVRVSAQMVDTQGRVLWSERFEDTPADVFALQDRITR
                     EIAGALAIRVTEFEQRRIATKPTESFDAYDYLLRARPALQRPSRAGIVEARELLRRAI
                     ALDPKYAAAHSALGETFHVAISMGWAESPDEYWKRVETHAGEALKFDSSDVRARILMA
                     RRYLAYNRHLEAQSQIDRAIGINPNDPDALAGRGNVLMWLGKTDAAIESLELALRIDP
                     ELNDFDRFALTLAYYLKQRYNEAIEQAELNLRRNPGARFNEAVLAAAYAQVGRGADAA
                     RVSENVRQTDPMFDPATYGNKFLNPKDLAALRDGLRKADLLVEAR"
     gene            complement(7323..7760)
                     /locus_tag="VEW08_02795"
     CDS             complement(7323..7760)
                     /locus_tag="VEW08_02795"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HYJ39689.1"
                     /translation="MLLTRRTATLAIAFALISSAGMAAEPAAVAIQGYDPVAYFTLQR
                     ATPGVAQFEYDWDEHRWRFANARHRDLFKADPVRYAPQFETFCAVSLARGEVHEASPE
                     YWLIIDDKLYLFGKSIGPELFRKDYKAMLERANRNRGLLPNKH"
     gene            7908..9209
                     /locus_tag="VEW08_02800"
     CDS             7908..9209
                     /locus_tag="VEW08_02800"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HYJ39690.1"
                     /translation="MMRIKSFCGLVLLALFGGVAPAYADAVVDWNEITVQAATAGRPG
                     PIAIVDIALVQVAVHGAVQAIDRQFEPYHAEIGTRRGDRAQGRRSAAVAAAAHDVLVG
                     MYPAQATTLDTTYFNYLADKGLGGDPGILIGQQVAARILPLRRANPNPLPPPFVGGTG
                     VGTWRPTPSFLGNPPSPAPFSPMATPWMGDFDPFTLTSPTRFRAPPPPALSSDRYTRD
                     YNEVKEFGSLVSKKRTPEQTDLAYFYVETAPVLWNRALRGIAARYLRKSGDTARLFAL
                     ANLATADALITCWDSKRFYAYWRPITAIQEGDADGNPATVGDPAWQPLINNPNYPDYT
                     SGANSVTGAMTRTLELFFGTDKMFFEVESAAPQAVQKIRRYNRFSDAARDVVEARILL
                     GIHFRFADLEARTQGRSVADWTFNHFLLPLGGRDQHGHDQW"
     gene            9203..10423
                     /locus_tag="VEW08_02805"
     CDS             9203..10423
                     /locus_tag="VEW08_02805"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HYJ39691.1"
                     /translation="MVIVSSYRAAALALLFTLAATARADVIMDWNARADAVAAEKRLP
                     PPLQGRALALMHVSMFEAINAIERRYKPYRLDLIADRNTSREVAAASAAHAVLTALYA
                     DQRPALDAQLAADLAAVAEGPAKERGIILGRKAAEELLELHAEDGAGADDYRPFTQAG
                     VYVPTQPVAGYHVRTFKPWVMASAAQFRAAPPPALTSETWTRDVNEIREVGALNSSVR
                     SPEQTTVGKFWFLTGARTYNPIVRQVVLAKQMNLLDCARIYALTSMAAADSFIAVFEA
                     KYHYNFWRPVTAIRNADLSQNPATPRDASWQPLGETPMHPEYPCAHCISASAVAATLR
                     AVVGDEVGEVTVTSNTAPGVTRRWTRLSDYADEVSSARIWAGFHYRFSTEAGKDMGRK
                     IGELTVATQLRPRK"
     gene            10462..11325
                     /locus_tag="VEW08_02810"
     CDS             10462..11325
                     /locus_tag="VEW08_02810"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HYJ39692.1"
                     /translation="MNNKLAIAVLVLFPLGAFAAEKPGDWRPLLDAKFSQFDVYLSYR
                     GDQIMSVIQGKAPADLKPVGLNPPGQNVFTMIEQGGKPVLRISGEIYGCATTREEFSN
                     YHFRASFKWGEKKWEPRLTELKDSGILYHSRGDFGVDYWKSWALSQEFQVIEHGIGEY
                     WRQATSAFDIRADAKAPGAEAPKWNPKAPWMAFAGSNNHALAGSNEDRPGEWNTLELV
                     CFADRCVHVVNGKVVMALRNSRYHDGGKDIPMTGGKLQIQSEAAEVFYRDLEIRTIPA
                     MPKEYEQYFRD"
     gene            complement(11315..12682)
                     /locus_tag="VEW08_02815"
     CDS             complement(11315..12682)
                     /locus_tag="VEW08_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ39693.1"
                     /translation="MITRSIALLLALTTLGACAYTPNKTDRLDIRQVPYTPDSGTIAL
                     RCGKLIDGNSDTAKRDVLVLIRDGKIKSLKEGASRSDAASTHVPVLDLSGYTCLPGLI
                     DMHTHLTDRPEDTADLTVYFSRPAEETLRLSKENASATLLAGFTSVRNVGTYALGTDT
                     ALRDAVNAGKAAGPRMQVSGPYLTIPQGGGDLYIPDFKEPPGSERFHAGVARGPDQFR
                     ERAEFLIDNGADLLKVIASGAVLAFGGVPGAPEMTQDEIQAVVDIAHAVGKKVAAHAH
                     GAESIRMAIAAGADTIEHASYLDDACIAAALEQGHVALAMDVYNGDYIDTEGRKAGWP
                     EEFLRKNTETTEIQRLAFTKAVKAGVPIVFATDSGVFPHGLNARQFPIMVARGMTPMQ
                     AIQSATRVAAQYMAWSARVGTIEPDKFGDVIAVKGDPLRDISALQDVAVVVKGGMLFK
                     NDISP"
     gene            complement(12679..13533)
                     /locus_tag="VEW08_02820"
     CDS             complement(12679..13533)
                     /locus_tag="VEW08_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HYJ39694.1"
                     /translation="MDVIDQRRRDLGGFEVGRVLPFTRRHMVGPFIFFDHIGPKHFSP
                     GFPKTVDVRPHPHIGLATVTYLFAGEIMHRDSVGVVQAIQPEEVNWMIAGRGITHSER
                     FEKARAEGGALHGIQAWVALPREHEETDPSFAHHDAAALPLHEDGGLRTRLLAGEAFG
                     LRAGVKTHSPLFYAHWEIASGARAALPEDYSERALYIAAGEVEVRGQVLGAGKMLVFD
                     KGEDTTVTASARSTIMALGGEPVGERFIDWNFVSSSKERIEQAKADWRAGRMKLPDFD
                     NQEFIPLA"
     gene            complement(13618..14913)
                     /locus_tag="VEW08_02825"
     CDS             complement(13618..14913)
                     /locus_tag="VEW08_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39695.1"
                     /translation="MKHRMVLLAAALIATSASATDRPQSFGVERHVLDQVREGRETFR
                     YSTFGDEAFWGDGLGLHLAIAGAANGGVGAGVSPAAALAAGLKVDLAALPRSLQRDIA
                     RGKVDLENPAVTLALLKLDAVVGVTGIFDERGRITSMGIQCALCHSTVDDSLTSGIGY
                     RRDGWANRDLNIGAIVALAPRLEPVATLLGTDVATVKKVLNSWGPGRFDASLFIDGKA
                     FRPDGKSGSVLIPPAFGLAGVNLHTWTGWGGVSHWNAFVANLEMHGKGTFIDPRLDDA
                     TRFPIAAREKFGHVRAEEDLITPQLPALQLYQLAIPAPAAPRGSYDWRAAKRGAAIFM
                     DKANCAACHVPPLFTEPGWNMHTGAEIGIDDFQSSRSPDGHYRTAPLRGLWTHMTGGF
                     YHDGRFPTLAAVIDHYDSHFSLGLSAGEKKDLAEYLKSL"
     gene            15026..15376
                     /locus_tag="VEW08_02830"
     CDS             15026..15376
                     /locus_tag="VEW08_02830"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYJ39696.1"
                     /translation="MDDQLSTTFAALADPTRRAILSRLTTGDASVTELAEPFDMSLPA
                     VTKHLKVLQRAGLISQGREAQWRPCRLQAKPMREAVEWMEPYRSHWEQRIDRLDDILR
                     ETTHKGARRPRKRK"
     gene            15378..15884
                     /locus_tag="VEW08_02835"
     CDS             15378..15884
                     /locus_tag="VEW08_02835"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYJ39697.1"
                     /translation="MSSHARSIKHGSFTIERHLNYEPSALFRAWTDPKAKARWFNGPA
                     DKWTELVREMDVRVGGRERVIGKFADGSESRFEAVYFDVVPDRRLVYTYDMYFQDKKI
                     SVSLASVEFVAKGKESRHETKLVVTEQHAFLDGYEDAGNRERGTLGLLANLEMALGGG
                     DPRVPRKT"
     gene            complement(15881..17005)
                     /locus_tag="VEW08_02840"
     CDS             complement(15881..17005)
                     /locus_tag="VEW08_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYJ39698.1"
                     /translation="MRTSREGYELKGISILCAAIFMNAAIAADPPAPVPPAVAPMVPG
                     SPIPWPEWLFPIDPKSLEKNPKPVKLDDVEQLEIPDSKLKFTQARINDPFNPPDWRPD
                     HSPAPDVVAKGRKPNVMACAYCHTPTGQGRPENSALAGLPEAYFKEQLHDLRGGARKP
                     AGPEQYLPTWNMLKIARAMTDEEIDESAKYFAQQKLRRRVYVIESLRIPRAERAAWIY
                     EEVGGTEDLEGRLLEVTNELSRHERRDDRLEYMAYVPPGSINRGKRLATTGKGPDGSS
                     PNAQETSREATTRQATNVDIEKTVVCATCHGPKLMGTDKIPPIAGRQPTYLLRQLLAF
                     RNGTRTGEAADQMKPVVDKLTLNEMIDMVAYVSSLYPDPR"
     gene            17033..17611
                     /locus_tag="VEW08_02845"
     CDS             17033..17611
                     /locus_tag="VEW08_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HYJ39699.1"
                     /translation="MPRISRRALPAATVELARYLIGKRLVHVTVAGRIAGRIVETEAY
                     PPGDPTGYARPGLTTSNAPLFGERGTAYVRMVYGTCLTLNLAAEAAGVGAAVLIRALE
                     PTEGIELMLGNRPDSRLVDLARGPGRVCAALGLSREWSGADLTAHPLLWLEPMPRQPA
                     AIQVTTRIGLSRDQHRRLRFVERGNPFVSVPP"
     gene            17620..18366
                     /locus_tag="VEW08_02850"
     CDS             17620..18366
                     /locus_tag="VEW08_02850"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYJ39700.1"
                     /translation="MRIAFGLTMLLASVAAHGDDRASPTEAAFRVALGVAENVNLFYR
                     NLDCSEMTFEAFTEAMHAEGVKSEVERAIDGSSVTLTVRRPGGNSCPSPYAPVTEMPP
                     FEMRDLAGKRVTSAALKGKPTLINFYFAQCVPCILEVGPINGYAAQHPEMNFLAVTFD
                     EAPVARAFVDRYQFRWRVAPDARDFIDRMKVKQYPMMALFDSSGRLLGTRRGGVRDEL
                     EAATVAPQLARWVDGLLRVNRKETSALSGK"
BASE COUNT         3368 a         5826 c         5919 g         3319 t
ORIGIN      
        1 cgttcacgag gaaactgacc cccagcaacg tcttgccgga acccggcccg cccgatacga
       61 gcgtgggtcg tccctgggga aaaccgccct tggtgatgtc atcgagtccc ggaatacctg
      121 ttggggcctt ctgaatttca gaacgctcta acttgtgctt gctcatttca aacctgggtc
      181 gttgcgtgga cggagtggac gcagtcgcgc tccgatccga cgcgccgcgt attgacgcag
      241 gatatttatg gccgggaaat cggcggagtc tcacaaccct gttagctgtt gtaggatttt
      301 tcggacaggc gtcgctcgcc tgtttcctgt cggaaatttc cgtaggcggg gcctcagcgg
      361 ggtatgggtg gggcggccct ctggaacacc ggcttgatgg cgagtcccga ctgaatgcgc
      421 gagacgcccg gaatcctcgt gagcacgtcg agcatgaagc gctggtagct aggaaggtcg
      481 ggcacgacga tacgcagtac gtagtcggct tcgccggtca tgaggtagca gctcatgacc
      541 tcgggtacgt cggcgatccg ccgctcgaac accttgagtg tttcctcgac ttgccgttcg
      601 agcgacaccc acacgtacat ggtgatgccg tatccgagct tctgttcgtc gaactgggcg
      661 ctgtagccgg tgatgacccc ctcgctctcc aggcgcctga tacgccgcaa cgtgggagtc
      721 agcgaaagtc cgatctggtc ggccaggtca cgccagctga tacgcccatc tttggacagc
      781 acctgcatga tcttcgcgtc gactttgtcg agtttttggg cagacatgcg ctatgcagcc
      841 ttgattgaga gggcaaacat gctaaaagac gccaaaaaat agtcaagaat tggcgttaac
      901 gcgctccggc agttacggta aattagctgc cgactcacgt aagcaactgt cgctcatcag
      961 gaatttcatg tcccgcaagc cgcccgtctc cgtttccttc gagttttttc cgccggccga
     1021 tgaagccatg gagaagaccc tgtgggagtc gatccgctat ctcgaaccgc tgcacccgcg
     1081 gttcgtctcg gtgacctacg gggcggacgg atccacgcgt gaacgcacgc acaatgtagt
     1141 gtcgcgcgtg cagaaagaga ccgatctcac gtgcgtgccg cacctcacct gcatcggcgc
     1201 cagtcgcggg gaagtgctcg acatcgcgcg caaatactgg gaccagggcg tccgccacat
     1261 cgtcgcgctg cgcggcgatg cgcccgcggg caccgtgaag tacgaaccgc gtgccgacgg
     1321 ctttgcctat gccgcggacc tcgtcgcggg tctgaagacg gtgggtgact tcgagatctc
     1381 cgtggccgcg taccctgaaa agcacccgga agcgccgagc gccgaattcg acctggatgt
     1441 gctcaagcgc aagatcgacg ccggcgccac ccgcgccatc acgcagttct tctacgacac
     1501 agatcttttc ctgcacttcc gcgacgcatg cgccgcgaaa ggcatcgact cgccgctcgt
     1561 gccgggcatc ctgcccatca cgcgttttcc gcaggtcacg cgtttcgcca agcgttgcgg
     1621 agcttcgata cctgagtggt tagagcaccg attcgacggc ctggaagaga accccgagac
     1681 gcgcaagctc atttccgcgg cggtggccat cgagcaggtg catacgctgc agcgtcacgg
     1741 cgtagaccag tttcacttct atacgttgaa ccgttccgag ctcacgtacg ccatctgcca
     1801 tgcgctcggc gttcgcccga aggcggcgtc gcgtaaggcg gccaatgagt aagccggttt
     1861 cggaagatca ggtgaaacgc gcccaggcgc ttcgccagct gttcgccgag cgtatcgtca
     1921 ttctcgacgg tgccatgggc accatgatcc agcagcacaa gctggacgaa gccgcgtttc
     1981 gtggcgagcg cttcaaggac tggccgcacg acctcaaggg ttgcaacgac ctgctggtgc
     2041 tgaccaagcc ggaactgatc gcggacattc atggccagtt tctcgaagcc ggcgcggaca
     2101 tcgtcgccac gaataccttc aatgcgactt cgctgtcact cgctgactac cacctcgaag
     2161 agctggtgta tgagctgaac tttgaagccg tgaagcttgc gcgccgcgtt gccgatgcag
     2221 aatcgaagaa gttgggtcgc acctgctacg tggcgggtgg cctggggccg accagcaaga
     2281 cggcttccat ttcacccgac gtcaatgatc ctggctatcg ggcggttacc ttcgatcaac
     2341 tcgtggcgac ctacagcgac gccacccgcg gtctcatcga cggcggcgcc gatgccattc
     2401 tcatcgaaac catcttcgat acgttgaacg ccaaggcggc tatctacgcg gtacgcgaca
     2461 cgctcgatgc ggccggcctg gatctgccca tcatcatctc cggcacgatc acggatgcat
     2521 cggggcgtac gctttccggt cagacggtgg aagccttctg ggcgtcggtg cgccatgcgc
     2581 gtccgatcac catcagcctc aattgcgcgc tgggcgtcaa acagttgcgg ccgtatgtcg
     2641 aggaactctc gcgcctcgcg gacacccacg tcggcgtgta tcccaatgcg ggcttgccga
     2701 acgcattcgg tgagtacgac gaggcgccgg aagacaccgc gaaggtgttg ggcgagctcg
     2761 cgcatgccgg ccacctgaat ttcgtcggtg gctgctgcgg caccacgccc gcacacatca
     2821 aggcgatcgc cgatcgcatg cgctgcgaac cggtgcgcaa gaagaaggat catgtgccgc
     2881 gcctggagct ctcggggctc gaggcgttca atctcacgga caacatccgt ttcgtgaacg
     2941 tgggcgagcg taccaacgtc acgggctcgg ccaggttccg caagctcatc gaggccaacg
     3001 attacccggc cgcgctgtcg attgcccgcc agcaggtgca ggacggcgcg cagatcatcg
     3061 acgtgaacat ggacgagggc atgctcgact cccaatctgc catggtgaag ttcctgaatc
     3121 tcatcgccgc cgagcccgac atcgcccgcg tgccggtgat gatcgactcg tcgaagtgga
     3181 gcgtgatcga agcgggcctc aagtgcatcc agggcaaggg cgtcgtcaac tccatcagcc
     3241 tgaaagaggg cgaagaggcc tttcttgcgc atgcgcgcaa ggtgcaggcc tacggcgccg
     3301 ccgtggtggt catggccttc gacgagcagg gccaggccga taccgtcgag cgcaaagtgg
     3361 gcatctgcga gcggtcgtac aaactactga ccgaaaagct cgacttcccg cccgaggaca
     3421 tcatcttcga tccgaacatt ttcgcgatcg ccaccggcat cgaagagcac aatggctacg
     3481 gcgtggcgtt catcgacgcc acgcgcgaga ttcgcaaacg ttgcccgcat gcgcatattt
     3541 ccggcggcgt cagcaacgtg tcgttctcgt tccggggtaa tgaacccgtg cgccaggcca
     3601 tgcactcggt gttcctgtac cacgccatcc aggcgggtat ggatttcggc atcgtgaacg
     3661 ccggccagct cgcggtttac gacgacattc cgaaagatct gcgcgagcgc gtcgaagacg
     3721 tggtcctcaa ccgccgcccg gatgcctccg agcgcctgct cgaaatcgcc gtcaactaca
     3781 aggacggtgg ggcgcgtgag cgcaaggaag atcttgcgtg gcggcaattg ccgattcgcg
     3841 agcggctcag ccacgcgctg gtgtcgggca acgatcaatt catcatcgaa gacactgaag
     3901 aagcccggct cgctgccgag catccgttgc atgtcatcga aggcgcgctc atggatggca
     3961 tgaacgtggt cggtgacctg ttcggctccg gcaagatgtt cctgccgcag gtagtcaaat
     4021 ccgcgcgcgt catgaagaag gccgtggcgc atctgatccc gttcatcgag gcgctcgccg
     4081 acggcgtgca gaagaccaac ggtaccgtgg tgctggcgac cgtcaaaggc gacgtgcacg
     4141 acatcggcaa gaacatcgtc ggcgtcgtgc tccagtgcaa caacttcgcc atcgtcgacc
     4201 tgggcgtcat ggtgcccgcg gacaagatcc tcgataccgc gcagaagttg ggcgccgaca
     4261 tgatcgggct ctcgggcctc atcacgccgt cactcgagga aatgagctac atcgccggcg
     4321 agatgcagcg ccgcggtttc acggtcccgc tgctcatcgg tggcgccacc acctcgccgg
     4381 cgcacaccgc ggtcaagatc gatccgaact atgaaggtcc cgtggtctac gtgaaagacg
     4441 cgtcgcgcgc cgtgggtgtg tgccgtcagc tcgtgacccc ggaaatgaag ccggcgtacg
     4501 tggccgaagt gaaagccgac aacgttcgcc gtcgcgagca gcatgcgggc aagaagcaga
     4561 agggcgcgca gctcacgctc gggcaggccc gcaacaaccg gccgaagatc gactgggcca
     4621 ggtacacccc gcccgtgccg cgtttcctcg gtgcgaaggt cttcaacgac gtcccgctgg
     4681 cggcgctcac gccgtacatc gactggatgc cgtttttcaa tgcctgggag tacggtggca
     4741 agttcccgga cgtgctccag gacccggtgc gcggcgaatc ggcgcgctcc ttatataagg
     4801 acgcccagga gatgctggcc accatcctgc gcgagcactg gctcacggcg cgcgcggtgg
     4861 tcgggttctt cccgtgcaac gccgtcgacg aagaaatcga ggtgtacagc gacaccacgc
     4921 gctcgcgcgt gctgcatcgc ctgcatcacc tgcgtcagca gaaggccaag ccggccggac
     4981 agccgcatta cgcgttgtca gatttcatcg ccccgaaaga ttcggggaag gtggactaca
     5041 tcggcgcctt tgccgtaacc gcgggcatcg gtatcgagcc gcatctcgaa cgcttcaagg
     5101 cggcgcacga tgattacaac gacatcctgc tgaagtcgct ggcggatcgg ctggcggaag
     5161 cttccgccga agcgctgcac ctgcgtgtgc gccgcgagtt gtggggctac gctcccgagg
     5221 agcggctcac caacgatcag ctcatccagg aagcctaccg cggcattcgt ccggcgccgg
     5281 gatatcccgc ctgcccggac cacaccgaga aaggcacgtt gtggcagatg ctcgatgtcg
     5341 agcgcgccat cgatctgcgc atcaccgaga gtttcgccat gtatccgacg gccgcagtca
     5401 gcggctggta cttcgcgcat ccggatgcca ggtacttcgc ggtcgggccc atcgacctcg
     5461 accagatgca gaactacgcc acgcgcaagg gctggactgc gcccgaagcc cgcaagtggc
     5521 tcatcagcaa tctggagaat cgcatctcag ccgatccgca cgacgaagcg gcctaggtgg
     5581 gtgccgggtg aggaaagtgg ggagacaatc cccactatcc ccacccggca ccaattgcga
     5641 tgatcgtgcg cgatgaccaa ccccgaggga gacaacgcgg cggatcaacg gctgaccttc
     5701 ggccgtttcg tactcgatct gcggcgtggc gtactgctgc tcgatggccg cgaggtcgtg
     5761 ttgcggccga aaacgttcgc ggtgctgagt cacctggtcc agcatccgga ccggctggtt
     5821 tccaaggaag aattgctcgc ggcagtctgg ccgaacctcg tggtcaccga cgacacgctg
     5881 gtgcagagca tcggcgagtt gcgccgcgcc ctcggagaaa ccggtgcgcg cctcatcgtc
     5941 acggtgccgc gccgcggcta tcgcttcgag gtttcggaag cgccgcctga ccggcgaaaa
     6001 gcccgcgcct ggcatccact gcgattccgc tggaactacg gcatcctcgc gccgctcgcg
     6061 gtcgccctca cggtgctggt gatgtggctc gccggattct ggagcgatgc acctcccgcg
     6121 gccgcagaag ccaagcccgc gatcgcggtg ctgccgttcc agcaccacgg cgaagatccc
     6181 gcgcgtgaat acctcgccga cggactcacg caggatctca tcaattccct ggggagattc
     6241 ccggcgctca cggtgatgtc atggaacgca gtcgccggct acaagggcga ggccgtgcgg
     6301 cccggcgaga tcgcgcgctc gctcgaggtg cggtaccagg tcgagggcag cgtgagctac
     6361 gccggggacc aggtacgggt cagtgcgcag atggtcgaca cgcagggccg cgtgttgtgg
     6421 tccgagcgtt tcgaagacac gcccgccgac gtgttcgcgc tgcaggatcg catcacgcgc
     6481 gagatcgctg gcgcgctggc gattcgcgtc accgaattcg agcaacgacg catcgcgacg
     6541 aagcccaccg aaagctttga cgcatacgac tatctattgc gcgcgcggcc ggcactgcaa
     6601 cgcccgtcgc gcgccggtat cgtcgaggcg cgggagttgt tgaggcgcgc catcgcgctc
     6661 gacccgaaat atgccgctgc gcattcggca ctcggcgaaa cctttcacgt ggcgatttcg
     6721 atgggatggg cggagtctcc cgacgaatac tggaaacgcg tcgaaacaca tgccggcgag
     6781 gccctgaagt tcgattcgtc ggatgtgcgc gcgcgcatcc tgatggcgcg caggtacctg
     6841 gcgtacaacc gtcatctgga agcgcagtcg cagatcgatc gcgccatcgg catcaatccg
     6901 aatgacccgg acgcgctcgc tggtcgcgga aacgtcctga tgtggctcgg caagaccgac
     6961 gccgccatcg aatcgctcga gctcgcgctg cgcatcgacc ccgagctcaa cgacttcgac
     7021 cgtttcgcgc tgacgctggc gtactacctc aagcagcgat acaacgaagc catcgagcag
     7081 gccgaactca accttcgcag gaatccaggt gcaaggttca atgaagccgt gctggctgca
     7141 gcctatgcgc aggtcggccg cggcgccgac gcggcgcgcg tcagcgaaaa tgtccggcag
     7201 accgacccga tgttcgaccc ggcgacctac ggcaacaaat tcctgaatcc gaaagacctg
     7261 gcggcactgc gcgatggcct gcgcaaggcg gacctgctgg tcgaagcgcg ctagccgcgc
     7321 gctcaatgtt tattcggcag tagcccgcgg ttgcgattcg cccgttcgag catggccttg
     7381 taatccttgc gaaagagctc cgggccgatg gacttgccga acaggtagag cttgtcgtcg
     7441 atgatcagcc agtattcggg gctcgcctcg tgcacttccc cgcgcgccag cgacacggcg
     7501 cagaaggttt caaactgcgg cgcgtagcgc accggatcgg ccttgaacag gtcgcgatgc
     7561 cgcgcgttgg cgaatcgcca gcgatgttcg tcccagtcgt actcgaactg tgcaacgccc
     7621 ggtgtcgcgc gctgcaacgt gaaataggcc accgggtcgt agccctggat ggcgacggct
     7681 gccggttcgg cagccattcc ggccgatgag atcagggcga aggcaattgc cagcgtggcg
     7741 gttcgtcgcg tgaggagcat ggcgccttta taacgcaggc cgtggccagc aggcagacgg
     7801 attcagtgaa gtttcgccag agtttccggg ttctttcgtt actgccgccc gctggctggc
     7861 cattctcgcg tctcgaaaac caacctgctc attcggaggg tcgaaccatg atgcgaatca
     7921 aaagtttctg cgggctcgtg ttgctcgcac tattcggtgg agttgccccg gcgtacgcgg
     7981 acgcggtggt ggactggaac gaaattacgg tgcaggccgc gaccgcgggg cgccccggtc
     8041 ccatcgcgat agtcgatatc gcgctcgtgc aggttgcggt gcacggtgcc gtgcaagcca
     8101 tcgatcggca attcgagccg tatcacgccg agatcggcac gaggcgaggc gacagggccc
     8161 aagggcgccg ctccgctgcc gtcgccgcgg cggcacacga tgtgctcgtc gggatgtacc
     8221 ccgcccaggc gaccactctc gacacgacgt atttcaacta cctggctgac aagggtctcg
     8281 gtggcgatcc cggcatcctc atcgggcagc aggtggccgc gcgaattctg ccgttgcgcc
     8341 gcgcgaaccc gaatcctctg ccgccgcctt tcgtcggggg gaccggcgtc ggcacctggc
     8401 gcccgacgcc gtcgttcctg ggaaacccgc cttccccggc accgttctca ccgatggcga
     8461 caccgtggat gggcgacttc gatccattca cgctcaccag cccgacgcgt ttccgtgccc
     8521 cgccaccacc ggcattgagc agcgaccgct acacgcggga ttacaacgag gtcaaggagt
     8581 tcggctctct cgtcagcaag aagcgcacgc ccgaacaaac ggatctcgcg tatttctacg
     8641 tcgagaccgc gcccgtgttg tggaaccgtg cgctgcgagg aatcgccgcg cgatacttgc
     8701 gcaagagcgg cgacacagcc cggcttttcg cgctggcaaa tctggcgacc gccgatgcgc
     8761 tcatcacctg ctgggacagc aagaggttct acgcctactg gcgacccatc acggccatcc
     8821 aggaaggcga cgctgacggc aatccggcga ccgtcggcga cccggcgtgg caaccattga
     8881 tcaacaatcc taactacccc gactacacct cgggcgcgaa cagcgtgaca ggcgccatga
     8941 cgcgcacgct cgagttgttc ttcggcacgg acaagatgtt cttcgaggtc gagagcgccg
     9001 cgccacaggc tgtgcaaaag atccgcaggt acaaccggtt ctcggacgcc gcgcgggacg
     9061 tcgtcgaggc gcgtatcctg ctcgggattc acttccgctt cgcggatctc gaggcgcgta
     9121 cccagggcag aagcgtggca gactggactt tcaaccactt cctgcttccg ctcggaggcc
     9181 gtgaccagca cggacacgat caatggtaat tgtcagcagt tatcgggccg ccgccctcgc
     9241 gttgctgttc acgctggccg ccacggcgcg ggccgacgtc atcatggact ggaacgcccg
     9301 ggcggacgcc gtcgccgccg aaaagcggct gcccccacct ttgcagggac gtgcgctggc
     9361 gctgatgcac gtgtcgatgt tcgaagccat caacgccatc gaacgccgct acaagccgta
     9421 ccggctcgat ctcatcgccg accggaatac ttcgcgcgaa gtggccgcgg cgagcgccgc
     9481 ccatgccgtg ttgacggcgt tgtatgccga tcaacgcccg gcgctcgatg cacagttggc
     9541 cgcggatctc gccgcggtgg ccgaggggcc ggcgaaggag cgcggcatca tcctcggacg
     9601 caaggcggcg gaggagctgc tcgagcttca cgccgaggat ggcgcgggcg ctgacgacta
     9661 ccgtccattc acccaggccg gtgtgtatgt gccgacccag ccggtggccg gttatcacgt
     9721 gcgcaccttc aagccatggg tcatggcgag cgcggcgcag tttcgcgccg cgccgccgcc
     9781 ggccctgacc tcggaaacct ggacgcgtga cgtcaatgag atccgggaag tgggtgcgct
     9841 caatagttcg gtgcgcagcc cggagcagac gacggtggga aaattctggt tcctcaccgg
     9901 tgcgcgaacc tacaacccca tcgtgcggca ggtggtgttg gctaagcaga tgaacctgct
     9961 cgactgcgcg aggatctatg cactcacctc gatggcggcg gcggactctt tcatcgccgt
    10021 cttcgaagcc aagtaccact acaacttctg gcggccagtc accgcgattc gaaacgccga
    10081 cctgtcacag aacccggcga cgccgcgcga cgccagctgg cagccgctcg gcgagacccc
    10141 gatgcatccc gagtatccgt gcgcacactg catcagcgcg tcggccgtgg ccgccacgtt
    10201 gcgcgcagtg gtgggcgatg aagtgggcga ggtcacggtc accagcaaca ccgcgccggg
    10261 cgtgacgcgc cgctggacgc ggctttccga ctacgccgat gaagtctcca gcgcgcgcat
    10321 atgggccgga tttcactacc ggttttcgac cgaggcgggc aaggatatgg gacggaaaat
    10381 cggtgagctc accgtggcga cacagcttcg accgcgcaag tagtgtttcc tggccggcgg
    10441 cggttgttat gctcgccgcc catgaacaac aagctcgcga tcgcagttct cgttctgttc
    10501 ccactcggcg cgttcgcggc tgaaaagccg ggcgactggc ggccgctgct cgatgcgaag
    10561 ttttcgcagt tcgacgtgta cctgagttat cgcggcgacc agatcatgag cgtgatccag
    10621 ggcaaggcgc cggcggatct gaagcctgtc ggcctgaacc cgccggggca gaacgtgttc
    10681 acgatgatcg agcagggtgg caagccggtg ttgcgcatca gcggtgagat ctacggctgt
    10741 gcgaccacgc gcgaggaatt ctcgaactac cacttccgcg ccagcttcaa gtggggtgag
    10801 aagaagtggg agccgcgcct gaccgagctc aaggattcgg gaatcctgta tcacagccgc
    10861 ggagacttcg gcgtcgacta ctggaagtcc tgggcgctgt cgcaggagtt ccaggttatc
    10921 gagcatggca tcggcgagta ctggcggcag gctacctcag ctttcgatat tcgcgctgat
    10981 gcgaaggcgc ccggcgccga agcaccgaag tggaatccca aagccccgtg gatggccttt
    11041 gccgggtcta acaaccacgc gctcgcgggc tcgaatgaag atcgcccggg cgaatggaac
    11101 acgctggagc tcgtgtgctt tgcggatcgt tgtgtgcacg tcgtcaacgg aaaggtggtg
    11161 atggcgctgc gaaattcgcg ctaccacgat ggcggcaagg acataccgat gacgggcggc
    11221 aagctgcaga tccagagcga ggcggcggaa gttttctacc gcgacctcga aatccggacg
    11281 attccggcga tgccgaagga atacgaacaa tatttcaggg actgatatcg ttcttgaaca
    11341 gcattccgcc tttcacgacc actgcgacgt cttgcagtgc cgagatgtcc cgcagcggat
    11401 cacctttcac cgcgatgaca tcgccgaact tgtccggctc gatcgtaccg acgcgcgcac
    11461 tccaggccat gtattgcgcg gccacgcgcg tggcggactg aatcgcctgc atgggcgtca
    11521 tgccgcgcgc caccatgatc ggaaactgtc gtgcattgag tccatgcggg aacacgccgg
    11581 agtcggtcgc gaaaacgata ggcactccgg ctttgactgc cttggtgaag gcgaggcgct
    11641 ggatttcggt ggtctcggtg ttcttgcgca ggaactcctc gggccagccg gcctttcgtc
    11701 cctcggtatc gatgtagtcg ccgttgtaca cgtccatggc gagcgcgacg tggccttgct
    11761 cgagtgcggc ggcgatgcat gcgtcatcca ggtacgaggc atgctcgatg gtgtcggcgc
    11821 ccgcggcaat ggccatgcgt atggactccg cgccatgcgc gtgcgcggcc accttcttgc
    11881 cgacggcgtg cgcgatgtcg accacggcct ggatttcgtc ctgcgtcatc tccggtgcgc
    11941 cggggacacc gccaaacgcc agcaccgcgc ccgaggcaat caccttgagc agatcggcgc
    12001 cgttgtcgat caggaactcg gcccgctcgc gaaattgatc cggcccgcgc gcgacgcccg
    12061 cgtgaaaacg ttcgctgccc gggggttcct tgaaatccgg gatgtacaga tcgccgccgc
    12121 cctgcggaat ggtgaggtac gggccgctga cctgcatgcg tggtccagcc gctttaccgg
    12181 cgttgaccgc gtcgcgcagc gccgtatcag tgccgagcgc ataggtgccg acattccgga
    12241 cgctggtgaa gccggctaac aaggtcgcgc tggcattttc cttcgacagg cgcagcgtct
    12301 cttcggcggg ccgcgagaaa tagacggtga gatccgccgt gtcctcgggc cggtcggtga
    12361 gatgcgtgtg catgtcgatg aggccgggca ggcaggtgta gccggacagg tcgagcaccg
    12421 ggacgtgcgt ggacgcggca tcgctgcggc ttgcgccttc cttgaggctc ttgatcttcc
    12481 cgtcgcgtat cagcacgaga acgtcgcgtt tcgcggtgtc ggagttgccg tcgatgagct
    12541 tcccgcagcg gagcgcgatg gtcccgctgt cgggcgtgta cggcacctgg cgtatgtcga
    12601 ggcgatcggt cttgttgggc gtgtaggcgc aggcgccgag ggtggtcagt gcgagcagga
    12661 gcgcgatcga acgggtgatc atgcgagcgg gatgaattcc tggttgtcga agtcggggag
    12721 tttcatgcgg cccgcgcgcc aatcggcctt ggcctgttcg atgcgctcct tcgaagatga
    12781 aacgaaattc cagtcgatga atcgttcgcc gacaggttcg ccgccgagcg ccatgatcgt
    12841 ggaccgggcg gatgcggtga cggtggtgtc ttcacctttg tcgaacacca gcatcttgcc
    12901 ggcgccaagg acctggcccc gcacctccac ttcgccggcg gcaatgtaca gggcgcgctc
    12961 ggagtaatcc tcgggcaggg cggcgcgcgc accgctggcg atttcccagt gagcgtagaa
    13021 cagcggcgaa tgggtcttca caccagcccg gagcccgaat gcttcgcccg ctaacaaacg
    13081 cgtgcgcagc ccgccgtctt cgtgcagcgg caatgcggcg gcgtcgtgat gtgcgaagga
    13141 cgggtcggtt tcctcgtgct ccctgggtag cgccacccag gcctgaatgc catgcagcgc
    13201 gccaccctcg gcacgcgcct tctcgaaacg ttcggaatgc gtgatgccgc ggccggcaat
    13261 catccagttc acttcttcgg gctggatagc ctggacaacg cccacgctgt cgcgatgcat
    13321 gatttcgccc gcaaacagat aggtgacggt ggcgaggccg atgtgcggat gcggccggac
    13381 atcgacggtc ttcggaaacc cgggagagaa gtgcttgggg ccgatgtgat cgaagaagat
    13441 gaaaggcccg accatgtgcc ggcgtgtgaa cggcagtacc cgtccgactt cgaacccgcc
    13501 gagatcccgc cgccgctggt cgatgacatc catgccatgt ggtttaccag accggcgggc
    13561 gcaggagcaa gaacggtgtc aggcaccgtt tcctaggaaa cggtgcctga caccaattta
    13621 caaggacttc aggtattcgg cgaggtcctt cttttcgccg gccgaaaggc ctaaagagaa
    13681 atgtgagtcg tagtgatcga tgacggcggc cagggtcggg aagcgcccgt cgtgatagaa
    13741 gccgccggtc atgtgcgtcc acagcccgcg aagcggagcg gtgcggtaat gaccatccgg
    13801 agagcggctc gactggaagt catcgatgcc aatctccgcg ccggtatgca tgttccagcc
    13861 gggttcggtg aacagcggcg gtacgtgaca cgccgcgcaa ttggccttgt ccatgaatat
    13921 cgccgcgccg cgctttgccg cacgccagtc atagctgccg cgaggcgcgg ctggcgccgg
    13981 aattgcgagc tggtacagct gcaacgccgg cagctgcggc gtgatcaggt cttcctccgc
    14041 gcgcacgtgg ccaaacttct cgcgcgccgc gatcggaaaa cgcgtggcgt catcgaggcg
    14101 cggatcgatg aacgttccct tgccgtgcat ctcgaggttg gccacgaacg cgttccagtg
    14161 cgacacgcca ccccagccag tccaggtgtg caggttgaca cccgcgagcc cgaacgccgg
    14221 tgggatcagg acagaccccg acttgccatc ggggcgaaac gccttgccgt cgatgaacag
    14281 cgatgcgtcg aagcgccccg gcccccagga gttcaagacc ttttttaccg tggcgacatc
    14341 agtgccgagc aacgtggcga ccggctcgag gcgcggcgcg agtgcaacga ttgcgccgat
    14401 gttgaggtcg cggttggccc agccgtcgcg ccgataaccg ataccgctcg tcagggaatc
    14461 gtcgacggtt gaatgacaca gcgcgcattg gattcccatc gaggtgatgc ggccgcgttc
    14521 gtcgaagatg ccggtgacgc cgaccacggc atcgagtttc aacagcgcga gcgtgacagc
    14581 gggattctcc aggtcgacct tgccgcgcgc gatgtctcgc tgcagcgagc gaggcagggc
    14641 ggcgaggtca actttgagtc ccgccgcgag cgcggcggca ggcgacacac cggcgccgac
    14701 acccccattg gcagcgccgg caatggcgag gtgtagtcca agtccgtcac cccagaaagc
    14761 ttcgtcgcca aaggtggaat agcgaaacgt ctcgcgaccc tcgcgtacct ggtcgagtac
    14821 gtgccgttcc acgccaaaag attgtggacg gtccgtcgcg ctcgccgagg ttgcgatcag
    14881 cgctgccgcc agcagaacca tgcggtgctt catttcagtt cccctgtcgg aaattttttc
    14941 tcccagccac aggtaaatgc acgcgcggtt gcaaatgttc cgcgccggtt ataatttacc
    15001 gatgggttaa ataacaaggt ggctcgtgga cgatcaactc agcaccacgt tcgcggcgct
    15061 cgccgatccc acgcgccgcg ccatcctgtc gcgcctcacg acgggagatg cgtccgtcac
    15121 ggaactggca gagcccttcg acatgagcct tccggctgtc accaaacatc tcaaggtcct
    15181 acagcgggcg ggcctcatca gccagggccg tgaggcacag tggcgcccat gcaggctcca
    15241 ggcgaaaccg atgcgtgaag cggtggagtg gatggagcct tatcggagcc actgggagca
    15301 gcgcatcgac cggctcgacg acattctgag agaaacgacc cacaagggag cacgccgtcc
    15361 ccgcaagagg aaatgacatg agctcgcatg caaggtccat caaacacggg tcgttcacca
    15421 tcgagcggca tttgaactat gagccgtcgg ccctgtttcg cgcgtggacg gatccaaagg
    15481 ccaaggcgcg ctggttcaat ggccccgcgg acaaatggac cgagctggtg cgcgagatgg
    15541 atgtccgggt cggaggccgc gagcgggtca tcggcaagtt cgcggacggc tccgagtcgc
    15601 gattcgaagc cgtgtatttc gacgtagtgc ccgatcggcg cctcgtctac acctacgaca
    15661 tgtatttcca ggacaagaag atttcggtgt ccctcgccag cgtggagttc gtggcgaaag
    15721 gaaaggaaag caggcacgag acgaaactcg tcgtgaccga gcagcacgca tttctggatg
    15781 gctacgaaga cgccggcaac cgcgaacgcg gcacgctcgg gctcctggcc aacctggaaa
    15841 tggcgctcgg cggcggggac ccgcgcgtgc cgcgcaagac ctaacgcgga tcggggtaca
    15901 gcgagctcac gtaagccacc atgtcgatca tctcgttgag cgtgagcttg tcgaccacgg
    15961 gcttcatctg gtcagcggct tcgccagtgc gcgtgccgtt gcggaaggcg agaagctgcc
    16021 ggagcaggta ggtaggctgc cggccggcga tcggcggaat cttgtcggtg cccatgagtt
    16081 tggggccgtg gcacgtggcg cacaccaccg tcttttcgat gtccacattc gtggcctgtc
    16141 gagtcgtcgc ttcacgggac gtttcctggg cattcggact cgagccatcg gggcccttgc
    16201 ccgtggtggc cagtcgcttg ccgcgattga tgctgccggg aggcacatac gccatgtatt
    16261 ccagccgatc gtcacgccgc tcgtgccggg agagttcgtt ggtcacctcg agcaggcggc
    16321 cctcgagatc ttcggtgccg cccacttcct catagatcca cgctgcgcgc tcggcccgcg
    16381 gtatgcgcag gctctcgatg acgtacacgc gccgacgcag tttctgttgc gcgaaatatt
    16441 tcgcgctttc gtcgatttcc tcgtcggtca tggccctggc gatcttgagc atgttccagg
    16501 tcggcaggta ttgctcgggc cccgcgggct tgcgcgcgcc accgcgcagg tcatgcagct
    16561 gctccttgaa atatgcttcc ggcaggccgg ccagcgcgga attttccggc cggccctgcc
    16621 cggtgggcgt gtggcaatac gcgcaggcca tcacgttggg tttgcggcct ttggccacga
    16681 cgtcgggcgc cggtgaatga tcgggccgcc agtcgggcgg gttgaacggg tcgttgatgc
    16741 gcgcctgcgt gaacttcagc ttgctgtcgg gaatttcgag ttgctcgacg tcatcgagct
    16801 tcaccggttt cgggttcttt tcgagagact tcgggtcgat gggaaacagc cactccggcc
    16861 atgggatggg gcttccggga accatcggag caacagctgg aggaaccggt gccgggggat
    16921 ccgcggcgat ggcggcgttc atgaatattg ccgcgcagag aatgctgatg cctttcaatt
    16981 cgtagccctc gcgagatgtg cgcatagtgc cacgggctgc atactcccgc ccatgccgcg
    17041 tatttcccgc cgggccttgc cggctgccac cgtcgagctg gcgcgttacc tgatcggcaa
    17101 gcgcctggtg cacgtgactg tcgccggccg gatcgccggc cgcatagtgg aaaccgaagc
    17161 ttacccgccg ggcgatccca ccggctacgc ccgccccgga ctaactacct cgaacgcacc
    17221 gctgttcggc gagcgaggca cggcctacgt gcgcatggtg tatggcacct gcctgacatt
    17281 gaacctggcg gccgaagccg ccggagtggg cgccgcggtg cttatccgcg ccctcgaacc
    17341 gacggagggc atcgagctca tgctcggaaa ccggccggat tcccgcctcg tggacctggc
    17401 gcgcggaccg ggacgagtgt gcgcggcact cggcttgagc cgcgagtggt cgggcgccga
    17461 tctcaccgcc catcctttgt tgtggctcga acccatgccg cgccagccgg ccgcgatcca
    17521 ggtgacgacc cgcatcggat tgtcgcgcga tcagcaccgc cggctgcgct tcgtcgagcg
    17581 cggcaaccct ttcgtgagcg ttccgccata atgcgtgcca tgcgaatcgc gtttggtttg
    17641 acgatgctgc tggcgagcgt ggccgcgcat ggcgacgatc gcgcatcgcc caccgaggcc
    17701 gcgtttcgcg ttgctctcgg tgtcgcggag aacgttaacc ttttctatcg caatctcgat
    17761 tgcagcgaga tgaccttcga agcgttcacc gaagccatgc atgctgaagg cgtgaaatcg
    17821 gaggtggagc gcgcgatcga tggcagttcg gtcaccctca cggtccggcg ccccggcggc
    17881 aattcatgcc cgtcgcctta cgcgccggta accgaaatgc cgccgttcga aatgcgcgac
    17941 ctcgccggca agcgtgtgac gtcggccgcc ctgaagggca agcccaccct catcaatttt
    18001 tatttcgcgc aatgcgtgcc gtgcatcctc gaggtggggc cgatcaacgg ctacgcggcc
    18061 cagcaccccg agatgaattt tctcgcggtg actttcgatg aggcgccggt ggcgcgtgca
    18121 ttcgtcgacc gttaccaatt ccgttggcgc gtcgcgcccg atgcgcggga tttcatcgac
    18181 cgcatgaaag tgaagcaata cccgatgatg gcgctgttcg attccagcgg ccgcctgctg
    18241 ggcacgcggc ggggcggggt acgcgacgaa ctcgaagcag ccactgtcgc cccgcaactg
    18301 gcccgctggg tcgacggcct gctgcgtgtc aaccgaaagg agacgagtgc gttgagcggg
    18361 aaataagccc atgtctgaga cgcaaaaacc ttccgccgcg gcgattgccg ccaaccgctt
    18421 cgggctcggt gc
//
LOCUS       DASQJS010000073         8943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4123067, whole genome shotgun sequence.
ACCESSION   DASQJS010000073 DASQJS010000000
VERSION     DASQJS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8943
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4123067"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1211
                     /locus_tag="VEW08_02855"
     CDS             252..1211
                     /locus_tag="VEW08_02855"
                     /inference="COORDINATES: protein motif:HMM:NF037181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39701.1"
                     /translation="MRLRGIGPEVLLLCFLIIGAAAFALGRRSARGRRLRRDVDYFAG
                     LDHLVNDRFDRATEVFTRLATKSHEADIQFALGSLMRKRGEVDRAIAIHSELANNTEP
                     LIREQATFALGLDYLSAGLMDRAEEKLRSLMGSERYRPAVLERLAWVYEQQREWRAAL
                     DLWRELPPEKQQELANVAAHYCCELGEVALAAGNLAEARAQVAAARVHAPDSARAALL
                     AARTAAAAGEAGKALEIFTAVLGDSRTIRIAFEPEARQVLKSQADALSAALRANPPRD
                     EADPPEPPRFRCEECGVASGTWHWRCPSCRNWDSLRTTQNRGI"
     gene            1297..1632
                     /locus_tag="VEW08_02860"
     CDS             1297..1632
                     /locus_tag="VEW08_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HYJ39702.1"
                     /translation="MSFNFKQFFPTLAATLLLTQFVQAEPVNINTADATALAKALNGV
                     GPAKAKAIVSYREKNGPFKTVDQLAMVEGITQKLIDKNRADLRLSADKAAGPQPAGQP
                     AVKPAKPAS"
     gene            1776..2663
                     /gene="galU"
                     /locus_tag="VEW08_02865"
     CDS             1776..2663
                     /gene="galU"
                     /locus_tag="VEW08_02865"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HYJ39703.1"
                     /translation="MKRAAKKSRVEVAVFPVAGRGTRFLPATKASPKEMLPIVDKPLI
                     QYAVEEALSAGARRLVFITGSSKRAIEDHFDSDGELENLLLKQGKSELLKMLKSVLPS
                     YASCIYIRQPAPLGLGHAVLCAKPAVGDAPFFVHLADDLIDAEEACLKQMSNRFDNGS
                     IVAVQTVPHSQTDKYGIVKTGSRHADLLRIEKIVEKPKPSVAPSNLAVVGRYLLTPRI
                     FTHLERIGEGAGGEIQLTDGIARLMNDEAVYAYRFAGKRFDCGSKLGYLQANVEYALA
                     HPELAGSFKSYLKKLSKEL"
     gene            complement(2682..3530)
                     /locus_tag="VEW08_02870"
     CDS             complement(2682..3530)
                     /locus_tag="VEW08_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39704.1"
                     /translation="MRNLLVLMSLASLAACGGGGGAGDPPPSSNSQPATVTPPVTTPP
                     STAAADLSLASRLYKGDERTPAGFDVEARPSSVTGTLSTRHIKNTDLATGPQAAGPTF
                     EVCTNDMAQAIDWSERQATWQGQYSDLVEIKSDTRMFEVIRVPRADVTAMIRHRVFRC
                     DYLDRSSTDLRVDVGAAGSMNQRPLNADELESLSEYLWQFTMFNNSDYAVESSARSAS
                     GDTLTQTIRMGQLVRGANGACDTVQLIDWTHTMNSVNGSLTRSLTNVRTFGVKSVSSV
                     AQSCSG"
     gene            complement(3533..3883)
                     /locus_tag="VEW08_02875"
     CDS             complement(3533..3883)
                     /locus_tag="VEW08_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39705.1"
                     /translation="MKLRAIQLPGNAGELLDSFIRVEGHLPGESHPLMQERQLTPSLE
                     KVAQRSLDDGHVWRAWTDDRAMWLWAGEVSLARSRERGLPVMEVRRYDESGLIEESGT
                     WVRVRNDSWQRCNE"
     gene            complement(4310..4801)
                     /locus_tag="VEW08_02880"
     CDS             complement(4310..4801)
                     /locus_tag="VEW08_02880"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HYJ39706.1"
                     /translation="MDAALTRDTLGRLLDEENASLSEFATLLNKEHGALRGRDIDALE
                     SLADARQASVVKLLKIEEERRSLCSMLGYETDLAGLAKLIAWCDPARTLAKRYEECAT
                     RARDCRNLNIRNGVLVGAHIRRVEGLLGAMTGASTEPRAYGPRGQSNPYAVSSGKVLS
                     AEA"
     gene            complement(4820..5146)
                     /gene="flgM"
                     /locus_tag="VEW08_02885"
     CDS             complement(4820..5146)
                     /gene="flgM"
                     /locus_tag="VEW08_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HYJ39707.1"
                     /translation="MANKISGIDGRPVQVGGGAPVAKVRNATSDGAKAETTGAASNID
                     VSDTARTLAALESHIGTLPVVNESRVDAVRRAVDDGRYHVDPQRVADKMLRFEVDLLA
                     TIPAHK"
     gene            complement(5278..6015)
                     /gene="flgA"
                     /locus_tag="VEW08_02890"
     CDS             complement(5278..6015)
                     /gene="flgA"
                     /locus_tag="VEW08_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HYJ39708.1"
                     /translation="MPVNSLTLSCLLVRCLMGIAVLGPTQANSQGIPAPAAQSLPAVR
                     SAAEAALRRQLDAGVTGVILEAAELDSRLRLAACPAPFAINATLPRGTQSRVMVRVAC
                     KSSVYWSVNVPVDIHRRTDVLVMRRAVGRGENIAVGDVLVQSRVIPGLTSPFVSRPAD
                     LAGRLTRRPIPEGTAVTADALDAALLIHRGQSVILAARTGGVEVSAPGVALADAAAEQ
                     RVRVRNLNSLKIIEGVADTAGVVRVNP"
     gene            6292..6702
                     /gene="flgB"
                     /locus_tag="VEW08_02895"
     CDS             6292..6702
                     /gene="flgB"
                     /locus_tag="VEW08_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HYJ39709.1"
                     /translation="MPLNLDQYVSVHAAALDVRARRGELIANNLANADTPGYQARDID
                     FRQAMARAAGDKTVGTTLATTQAGHIGGAASASAATNPDLKYRTPLAPALDGNTVDAQ
                     IEQASFAENAVRYQATLTFLNSKFRGLLTAILGN"
     gene            6719..7144
                     /gene="flgC"
                     /locus_tag="VEW08_02900"
     CDS             6719..7144
                     /gene="flgC"
                     /locus_tag="VEW08_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HYJ39710.1"
                     /translation="MSSFKAFDIAGSGMAAQSVRLNTTASNLANADSVSGTAANVYKP
                     RHPIFEAVRAQLAAQGASESQPAVRVRGIYESTAAPSQRFEPGNPLADTNGYVYAPAV
                     NVVEEMVNMISASRSYQNNVEVMNTSKDLLLATLRLGQG"
     gene            7159..7860
                     /locus_tag="VEW08_02905"
     CDS             7159..7860
                     /locus_tag="VEW08_02905"
                     /inference="COORDINATES: protein motif:HMM:NF025230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HYJ39711.1"
                     /translation="MDIINAAMVRQRSPEEVAAAAMAKEANAKKTELNQEDFLTLMIT
                     QLKNQDPFKPMDPAQYVGQLAQFSSVSGLAEVNKNISTLTSSLRGNQVLDGAALIGRT
                     VIAEGNQVYRYVPEEGEAIPTQGAVEVPAGASSVQLIVRDSAGTLVKSQAMETQKGLR
                     GFIWDGSTDAGTTAPTGAYKIEVVAKVGDKNVSLNTSMAAQVSSVALDPATGSLMLDT
                     DTLGEIAMSEVERVL"
     gene            7891..>8943
                     /locus_tag="VEW08_02910"
     CDS             7891..>8943
                     /locus_tag="VEW08_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HYJ39712.1"
                     /translation="MPFRIALSGLNAAQTDLGVTANNIANTSTNGFKGSRAEFADLFA
                     VSLQGVSSNATGNGVRVAAVSQQFAQGNIEFTDSNLDLAVSGPGMFIVADGGALAYTR
                     AGSYQLNRDGFVVNSQQQRLQAYPPLVDGTFNTGGLGDLRLQTTDSPPAATTNVEYLL
                     NLPANATPPLIAPFDPTNPNTFNQATSLTLFDSLGATHTGTMYFEKTVNPNEWTSRLY
                     IDGTAVGAPNTLQYSNGGLLTSPVGGILPATAYTPTTGAADMTVAVDLRQSTQYGSNF
                     SVNAVTQDGYTTGRLIGIDINETGVVQARFTNGISQPLGQIAIANFANVQGLQQLGNT
                     QWAETFASGQALRGQAG"
BASE COUNT         1671 a         2929 c         2835 g         1508 t
ORIGIN      
        1 accacgatca ccgcatcgcc atgtctttcg gcgtgctggg atcgcgcctt ccgggcctgc
       61 ggattctcga tccgggctgc gtggcgaaga cttacccgaa ctattggcag gactggcagc
      121 gcgcccgaag tgccagcaga aaaggggaca gatctattta attggcgatt ccgcgagggc
      181 cagcgctaga tgtcgggtcg acgacatcgc caattaaata gatctgtccc cttttctgct
      241 aatttcctcc catgcgcctg cgtggcatcg gtcccgaagt tctcctgctg tgcttcctca
      301 tcatcggggc ggccgcgttt gcgctcggac gccgttcggc gcgcggccgg cggctgcgcc
      361 gcgacgtcga ttatttcgcc ggcctcgatc acctggtgaa tgaccgcttc gatcgcgcga
      421 cagaggtgtt cacgcggctc gcgacgaagt cgcacgaggc ggacatccag ttcgcgctcg
      481 gcagtctgat gcgcaagcgc ggcgaggtcg atcgagccat tgccatccat agcgagctcg
      541 ccaacaacac cgagccactg attcgcgaac aggccacctt cgcgctgggc ctcgattacc
      601 tgagcgcggg cctcatggat cgcgccgagg aaaaactgcg cagcctgatg ggatccgagc
      661 gctaccggcc cgccgtgctc gagcggctgg cctgggttta cgagcagcaa cgcgagtggc
      721 gtgcggcgct cgacctgtgg cgcgagctgc cgcccgaaaa gcagcaggaa ctggcgaacg
      781 tggccgcgca ttactgctgc gagctcggcg aagtggcact ggccgcggga aatcttgccg
      841 aagcgcgcgc gcaagtggca gccgcacgcg tccatgcccc ggactcggcc cgcgcggcgc
      901 tgctggcggc gcgcaccgcg gcggccgcgg gtgaggccgg caaggcgctc gagatcttca
      961 cggccgtgct cggcgattcg cgcaccatcc gcatcgcgtt cgagccggaa gcccggcagg
     1021 ttctcaagtc acaggccgat gcgctcagcg cagcgctgcg cgccaacccg ccgcgcgacg
     1081 aagcggaccc gccggagccg ccgcgtttcc gttgcgagga gtgtggcgtg gcgagcggca
     1141 cctggcattg gcgctgcccg agctgtcgca actgggactc cctgcgcact acgcaaaatc
     1201 gcggcatttg accaaattgg cggctgccgg cgacgaccgc aacgttgtta gtctccttcg
     1261 tacccggcag gtccgggcac acggcaagga ggccaaatgt ctttcaactt caaacaattc
     1321 tttcccacgc ttgctgccac gctgttgctc acgcagttcg tgcaggctga gcccgtcaac
     1381 atcaacaccg ccgatgccac ggcgctcgcc aaggcgctga acggcgtcgg gcccgccaag
     1441 gcaaaggcca tcgtgagcta ccgcgagaaa aatggtcctt tcaagaccgt cgatcagctc
     1501 gccatggtcg agggcatcac gcagaagctc atcgacaaga atcgcgccga cctcaggctt
     1561 tcggcggaca aggccgcggg tccgcagccc gccgggcagc cagcggtcaa gccggcaaag
     1621 cccgcgtcgt aacgcgcgga ccggcgcccg tggccgcgag agtagtgaag tagcgcagac
     1681 tctcgcgtgc ccgggtcggt tgcctgcaga gcggaaaatc gcggcgtggt atggtttcgg
     1741 cccctaactg ggccggagcc catatgcccg cagtcgtgaa gagagccgcg aagaagagcc
     1801 gtgtggaagt tgccgtcttc ccggttgccg ggcgaggaac ccgtttcctg ccggccacca
     1861 aggccagccc caaagagatg ctgcccatcg tcgacaaacc actcattcaa tatgcggtgg
     1921 aagaggcgct gtcagccggc gcacggcgcc tggtgttcat cacaggttct tccaagcgcg
     1981 ccatcgaaga tcacttcgat tccgatggcg aactcgaaaa cctcctgcta aagcagggca
     2041 aaagcgagct gctcaagatg ctcaagagcg tgctgccgtc atacgcctcc tgcatctaca
     2101 tccgccagcc cgcgccattg ggattgggtc acgccgtgct ttgcgccaaa cccgcggtgg
     2161 gtgatgcgcc gttcttcgtg cacctggccg acgacctcat cgacgccgaa gaggcgtgcc
     2221 tgaagcagat gagcaatcgc ttcgacaacg gcagcatcgt ggccgtccag accgtgccgc
     2281 actcgcagac ggacaagtac ggcatcgtga agaccgggtc ccgccacgcg gacctgctgc
     2341 gcatcgaaaa gatcgtggaa aagcccaagc cctcggtggc gccttcgaat ctcgcggtgg
     2401 ttggccgcta cctgctgacg ccgcgcatct tcacgcatct cgaacgcatc ggcgaaggtg
     2461 cgggcggcga gatccagctc accgacggca ttgcgcggct catgaacgac gaggccgtgt
     2521 atgcataccg cttcgcgggc aaacgcttcg actgcggcag caagctcggt tatctgcagg
     2581 ccaacgtgga atacgcgctc gcgcacccag agctcgcggg gtcgttcaag tcgtatctga
     2641 aaaagctctc gaaggagctt tgaaggcggg cgaggctgga atcagcccga gcagctctgc
     2701 gcgactgagg acacgctctt tacgccgaat gtacgcacgt tcgtgagcga gcgcgtcagc
     2761 gagccgttga ccgaattcat ggtgtgcgtc cagtcgatga gttgcacggt gtcgcaggcg
     2821 ccattcgcac cgcgcaccag ctgacccatg cgaatcgtct gcgtcagcgt gtcgccgctg
     2881 gccgaacgag cactgctttc gacggcgtag tcggagttgt tgaacatcgt gaactgccag
     2941 agatattcgg acagggactc gagctcatcg gcattcagcg ggcgctggtt catcgaaccc
     3001 gccgcgccca catcgacgcg cagatcggtg ctcgagcgat cgagatagtc gcaacggaac
     3061 acgcggtggc gaatcatcgc cgtcacgtca gcgcgcggca cacgaatcac ttcgaacatt
     3121 cgcgtgtcgc tcttgatctc gacgagatcc gaatactgtc cctgccaggt ggcctggcgc
     3181 tccgaccagt cgatggcctg cgccatgtcg ttggtgcaaa cctcgaaagt ggggccggcg
     3241 gcctgggggc cggtggccag atcggtgttc ttgatatgtc gcgtcgacag tgtgccggtg
     3301 acgctcgagg ggcgagcttc gacatcgaag ccggccggag tgcgttcatc gcccttgtag
     3361 agccgcgagg caagcgagag atccgcggcc gccgtcgacg gcggtgtggt cacgggcggc
     3421 gtcaccgtgg caggctggga attcgaggag ggcgggggat cgccggcgcc accaccaccg
     3481 ccgcacgcgg cgagggaggc gaggctcatc aatacgagaa gattgcgcat attcattcgt
     3541 tacaccgctg ccagctgtcg ttgcggacac gcacccaggt gccgctttct tcgataagcc
     3601 ccgattcatc gtaacgccgt acttccatga ccggcaggcc gcgttcgcgc gagcgcgcca
     3661 gcgacacttc gccagcccac agccacatcg cgcggtcgtc cgtccaggcg cgccacacgt
     3721 ggccatcgtc cagggagcgc tgcgcgacct tctcgagcga cggcgtcagc tggcgttcct
     3781 gcatcagcgg atgcgactcc cccggcaggt ggccctcgac gcggatgaag ctgtcgagca
     3841 gctcaccggc attgccgggc agctggatcg cgcggagctt catcgtttcc atcgtcgaac
     3901 gttcccttta tcgttgttag gggccagcgg cagcacacga gcacttgcga gagaggagta
     3961 ccgtcggtcg gttgcacgga cccgcgagct gccgccagcg agacgttggt acctctgcaa
     4021 aaatgcttca gcaccctgaa agggttttcg gcggaaatcc ggccggattg agccgccgaa
     4081 tgtgaccggc ttcgcgggtg agcggcaatt atttgaacct cgtcacttat gttgtgttgt
     4141 taggcgtgag gccgtctcgc gatgcgcgac gtgtttcacg ccatcccctt attaggtaaa
     4201 tgaggctaat cccgctgctc cacgtggaac ataggtagtt agcgcaatga aaaaaacgcg
     4261 ccggaggcgc gttttcgagg gatggcggtc aagagaccag ggaggccgct caggcttctg
     4321 cggagagaac cttgccggag gagacggcgt agggattcga ctggccgcgc gggccatagg
     4381 cgcggggctc ggtggaggcg ccggtcatgg cgcccagcaa tccttcgacg cgcctgatgt
     4441 gcgcaccgac caggacgcca ttgcgaatgt tgagattgcg gcagtcgcgg gcgcgggtgg
     4501 cgcattcttc gtagcgcttg gcgagcgtgc gggccgggtc acaccaggcg atcagcttgg
     4561 caaggccggc gagatcggtt tcgtagccga gcatgctgca gaggctgcga cgctcttctt
     4621 cgatcttgag taatttgacg acgctggcct ggcgcgcatc ggccagcgat tccagggcgt
     4681 cgatatcacg gccgcgcaat gcgccgtgtt ccttgttcag cagggtggcg aactcgctca
     4741 aactggcgtt ttcctcatcg agcaggcgcc cgagggtgtc acgagtcagg gcggcatcca
     4801 cgtaggtctc gctccgggac tacttgtgtg cggggatcgt ggcgagcaga tcgacttcga
     4861 agcgcaacat cttgtcggcg acgcgctgcg gatcgacgtg atagcggcca tcgtcgacgg
     4921 cacggcgcac ggcatcgaca cggctctcgt tcaccaccgg cagcgtgccg atgtggcttt
     4981 cgagggcggc gagggtgcgc gcggtgtcgg agacgtcaat gttcgaggcg gcaccggtgg
     5041 tttcggcctt ggcaccatcg gaagtcgcat tgcgcacttt ggcaacgggg gcaccgccac
     5101 cgacctgcac cggacgtccg tcaattccac tgattttgtt cgccatcgcc cattctccaa
     5161 ggggtatcta cgaccctctt aacggcccct ggttttgaaa ctttaggccg tccgacgaaa
     5221 aattggcgct ttcggggtcg cctggcgtga cgcagttccc ccgaggttgc ataggcatca
     5281 tgggttcacc cgcaccacac ccgcagtgtc ggccactccc tcgattatct ttaatgagtt
     5341 gagattccgg acccgaaccc gctgttcggc ggccgcgtcc gccagggcca cgcccggggc
     5401 gctgacttcc acgcccccgg tgcgggcggc gaggatgacg ctctggcccc tatgtataag
     5461 gagtgcggcg tcgagggcat cggcggtcac ggccgtgcct tcggggatcg gccggcgagt
     5521 caggcgtccg gcgaggtccg cgggacgcga gacaaaaggc gatgtgaggc cggggatgac
     5581 gcgggactgc accagcacat cgccaaccgc gatattttca ccgcggccca ccgcacgccg
     5641 catcaccagc acgtccgtcc tgcgatggat atcgaccgga acattgacgc tccagtagac
     5701 actgctcttg caggccacgc gcaccatcac gcgggactgg gtgccacgcg gcagcgtggc
     5761 attgatcgcg aacggcgccg ggcaggcggc cagccgcagg cgagagtcga gctccgcggc
     5821 ctcgaggatc acgcccgtca cgcctgcgtc gagctggcgg cggagcgccg cttcggcggc
     5881 gctgcgcacg gcaggcagcg actgggcggc aggagccgga atgccctgcg aatttgcctg
     5941 ggtcgggcca agcacggcaa tgcccatcag gcaccggacg agcaaacagg aaagggtcaa
     6001 tgaattgact ggcatgcact gtagttagca atcgacgtgc catgcgacgg cgcattcaag
     6061 aaaagaaccg cgtggccgtt acgggaatac gacgaagtcc tggctcgcgc ctgcaggtgc
     6121 tggccgcgta gcggcaatga gccgccacca ccgcgtgcgc tgcgtcgcag acttttccct
     6181 ccgttccgat tgaaacgccg cccacagcgc caaaacgccc tgtggcacgt cacttgcttt
     6241 gctcctcgct tgtcacccgc catgacgcat ggccgacacg aggagtaagc gatgcccctg
     6301 aacctggacc agtacgtgag cgtgcacgcc gcagcgctcg acgtgcgcgc gcgccgcggc
     6361 gaactgattg ccaataacct ggccaatgcc gacacgcccg ggtaccaggc ccgtgacatc
     6421 gatttccggc aggccatggc tcgagccgcg ggcgacaaga ccgtcggcac cacgctcgcc
     6481 accactcagg cgggccacat cggtggcgcc gcgtcggcca gcgccgccac caatccggac
     6541 ctcaagtacc gcacgccgct cgcgcccgcg ctcgatggca acaccgtcga tgcgcagatc
     6601 gagcaggcct cgttcgccga gaacgccgtg cgctaccagg caacgctcac tttcctcaac
     6661 tcgaaattcc gcggtttgct gaccgccatt cttggcaact aagggttggt agctagtcat
     6721 gtcatcattc aaagctttcg acatcgccgg atccggcatg gccgcgcaat ccgtgcggtt
     6781 gaacaccacc gccagcaatc tcgccaacgc cgacagcgtc agcggtacgg cggcgaacgt
     6841 ctacaagccg cgccacccga tcttcgaggc ggtgagagcg cagctggcgg cgcagggcgc
     6901 ctccgaatcg cagcccgcgg tgcgcgtgcg cggcatctac gaatccaccg cggcgccgag
     6961 ccagcggttt gagccgggaa atccgctggc cgacaccaac ggctacgtgt acgcgccggc
     7021 ggtcaacgtg gtggaagaga tggtcaacat gatctccgcc tcgcgttcgt accagaacaa
     7081 cgtcgaagtc atgaacacct ccaaagacct gctgctggcc acgctgcggc tcggacaagg
     7141 ctgaacacta tgttagacat ggacatcatc aatgccgcga tggtccgtca gcgctcgccc
     7201 gaagaagtcg ctgccgccgc gatggccaaa gaggccaacg ccaagaagac cgagctcaac
     7261 caggaagatt tcctgacgct catgatcacg cagctcaaga accaggaccc gttcaagccc
     7321 atggatccgg cgcagtacgt gggccagctc gcgcagttca gctcggtgtc cggcctcgcg
     7381 gaagtcaaca agaacatctc gacgctgacc agttcgctgc gcggcaacca ggtgctcgac
     7441 ggcgccgcgc tcatcggccg cacggtgatt gccgaaggca accaggtcta tcgatacgtg
     7501 ccggaggagg gcgaagcgat acccacgcag ggcgcagtcg aagtgcccgc gggcgcgtcg
     7561 agcgtgcagc tcatcgtgcg cgattcggcc ggtacgctgg tcaagtcgca ggcgatggaa
     7621 acccagaagg gcctgcgcgg cttcatctgg gacggcagca ccgacgccgg caccaccgcg
     7681 ccaaccggcg cctacaagat cgaagttgtc gccaaggtcg gcgacaagaa cgtttcactg
     7741 aacaccagca tggcggccca ggtttcgagc gttgcgctcg acccggccac cggctcactc
     7801 atgctcgaca ccgataccct cggcgaaatc gccatgagtg aagtcgagcg cgtgctgtaa
     7861 ccagaaccag aattctcaag gagactctcc atgccttttc gcattgccct ctcgggcctg
     7921 aacgccgccc agaccgatct tggcgtcacg gcaaacaaca ttgccaacac ctccacgaac
     7981 ggcttcaagg gctcacgcgc tgaattcgcc gacctgttcg ccgtttcgct gcagggcgtg
     8041 tcgagcaacg ccaccggcaa cggtgtgcgg gtcgccgcgg tttcgcagca gttcgcgcag
     8101 ggcaacatcg agttcaccga ttcgaacctc gacctggccg tcagtggtcc gggcatgttc
     8161 atcgtcgccg acggtggcgc gctcgcctac acgcgcgccg gttcctacca gctcaatcgc
     8221 gacggcttcg tggtgaactc gcagcaacag cgcctgcagg cctatccacc gctggttgac
     8281 ggcaccttca acaccggtgg actcggcgac ctgcgcctgc agaccaccga ttcgccgccc
     8341 gcggccacga ccaacgtcga atacctgctg aatcttcccg cgaacgccac gccgccgctg
     8401 atcgcgccgt tcgatccgac gaatccgaac acgttcaacc aggcgacttc actcacgctg
     8461 ttcgattcgc tgggcgcgac gcacaccggg acgatgtatt tcgaaaagac cgtgaatccg
     8521 aatgagtgga cctcgcgcct gtacatcgac ggcaccgcgg ttggcgctcc gaatacgctg
     8581 cagtattcca acggtggcct gctcacgtcg ccggtcggcg gcatattgcc ggctacggcc
     8641 tacacgccca ccacgggcgc ggccgacatg accgtggcgg tggatctgcg ccagtccacg
     8701 cagtacggca gcaatttctc ggtcaacgcc gtgacgcagg acggctacac caccggccgc
     8761 ctcatcggca tcgacatcaa tgaaacgggc gtcgtacagg cccgcttcac caacggcatc
     8821 tcgcagccgc tcggccagat cgccatcgcg aacttcgcca acgtgcaggg cctgcagcag
     8881 ctcggcaaca cgcagtgggc cgagacgttc gcctcgggcc aggcgctgcg cggccaggcc
     8941 ggc
//
LOCUS       DASQJS010000074        11492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4439736, whole genome shotgun sequence.
ACCESSION   DASQJS010000074 DASQJS010000000
VERSION     DASQJS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11492
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4439736"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..2176)
                     /locus_tag="VEW08_02915"
     CDS             complement(80..2176)
                     /locus_tag="VEW08_02915"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HYJ39713.1"
                     /translation="MENRDAPKNRLARETSPYLQQHAANPVHWYAWGPEAFEAARDSG
                     KPVLLSIGYSACHWCHVMAHESFEDTATAALMNELFVNIKVDREERPDIDKIYQFAHQ
                     VLTQRGGGWPLTMFLTHDDQKPFFGGTYFPDKSRYGMPAFSTLLKRVSEYYRDQRDEL
                     RAQNDTLMDVFGDLTPAPVDASESLTAVPLMAAREQLNATFDKRYGGFGDAPKFPHPA
                     SIDRLMHHWHSTSVSQAPDLHALYMATLTLTRMGEGGMYDQLGGGFARYSVDQFWMIP
                     HFEKMLYDNGALLATYAEAALATGDPLFKRIANETGAWMLREMQDEDGGFYSAYDADS
                     EGHEGKFYVWTRTEVRAALSPIEYSVFSRRFGLDEEPNFEGVWHAHVFVSIEQIGKEL
                     SLEPSEVEKFVDSARARLLAIRAKRVWPGLDDKVLTSWNALAIRGLAIAARALEKPEF
                     AEAAERALAFTRTHLWRPGSIGGGRLLATAKDGVAHLNAYLDDYAYLANALLEMLQLR
                     WRNEDAAWLRQILDAMLEHFEDAELGGFFFTSDDHEALIHRSKSFSDDAIPAGNGIAA
                     RALIRAGYLLGETRWLEAAERTLRAAWLAINRFPHGHMSLLEALAEYLAPPEIVIIRE
                     ISDAGAWQRELGKLYAPHRMVFSIPATIEGLDAAILDKKPGASSRAYVCRGSTCSAPV
                     ETLADLVRTAAARVAN"
     gene            2197..3390
                     /locus_tag="VEW08_02920"
     CDS             2197..3390
                     /locus_tag="VEW08_02920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019097921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HYJ39714.1"
                     /translation="MTTPVLKLKRGEDRRLRAGHLWVFSNEVDTAATPLTQFEPGAAV
                     QVHSDKDQFLGHAYINPRTLIAARIVGRDPSYPLDASLLVHRLRVALALRERLYREPY
                     YRLVYGESDGLPGLVLDRYGDVVVAQSGTAGIDRLRAEVEDAVNKVIHPKAIVWKNDS
                     GARELEGLERGVSLAGEGEIPTDISVREQGVDFIAPLADGQKTGWFYDQAANRERLRR
                     YLPAGARVLDVCSYVGAWAITALKGGAASGTCVDSSATALDYVSRNARANGVEVETLK
                     DDAFDALKALQESGARFDVVILDPPAFAKRKKDVHQAQAAYRKLNQLALPLIDRDGLL
                     VSCSCSYHMEADELVNAIQTAARHTSRFVQVLEQGGQSPDHPVHPAIPETRYLKSFFC
                     RVTRE"
     gene            3406..4242
                     /gene="lgt"
                     /locus_tag="VEW08_02925"
     CDS             3406..4242
                     /gene="lgt"
                     /locus_tag="VEW08_02925"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HYJ39715.1"
                     /translation="MIPYPSFDKIAFEIGPFGSFGPLKVHWYGIMYLVAFAAGWFLAR
                     LRAKKPGSTWKPVDVDDFLFFCMLGVILGGRLGYVFFYGMSYWALDPWYPLKIADGGM
                     SFHGGLIGVMVALAVFAVRRKRRIADVFDFAAPLPGIGLCAGRIGNFINGELWGKPTD
                     APWGFMVNGEGRHATQLYEAALEGVVLFLIMWWYTSRPRPRMAPAGLFLILYSLARIT
                     VEFWRLPDSHLNEASGGYLVGHWFTMGMLLSLPMLLIGATLMFMAYRRRETSGNFSAV
                     PA"
     gene            4247..5041
                     /gene="thyA"
                     /locus_tag="VEW08_02930"
     CDS             4247..5041
                     /gene="thyA"
                     /locus_tag="VEW08_02930"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HYJ39716.1"
                     /translation="MKQYLDFMRHVRRNGAQKADRTGTGTLSVFGHQMRFDLAAGFPL
                     VTTKRVHFKSIAYELLWFLRGETNTKYLREHGVSIWDEWADANGELGPVYGKQWRSWP
                     ARDGRHIDQIAQVVEQLRTSPDSRRIIVSAWNVGELDQMKLMPCHAFFQFYVSDKRLS
                     CQLYQRSADIFLGVPFNIASYALLTHMLAQQCDLEVGEFIWTGGDCHLYSNHLEQVDE
                     QLSRVPLTLPKLVLTRRPPSMSDYEFEDFQIVNYQHHPAIKAPVAV"
     gene            5038..5697
                     /locus_tag="VEW08_02935"
     CDS             5038..5697
                     /locus_tag="VEW08_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HYJ39717.1"
                     /translation="MKKKKKVKPFDQSQPKRGGPPIPTSPLVEVRNSDVHGRGVFAVA
                     PIAKGTRIFEYLGDRVSHAAADLRYEDHDENDNHTFLFIVDRNTVIDAAVNGSDARFI
                     NHHCDGNCTSVIEHRRVFIDSLRAIAPGEELGYDYEIGRDKDDPPNVDEIYACRCGSP
                     KCRGSMLEAAPKKKAARPKPKPKAKRRKPEKARQRTRQSTHKGRRAETKRRGERPRRR
                     A"
     gene            5727..6284
                     /locus_tag="VEW08_02940"
     CDS             5727..6284
                     /locus_tag="VEW08_02940"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HYJ39718.1"
                     /translation="MADDFVDAKLLAELRARCIELHQTGGLRPARVGRGDNAQLVPEV
                     RGDFISWLETPERDAEQRVLTHLDELRSTLNRALMTGLEDFQGHFALYPSGAGYARHF
                     DRLVGNDVRAISAALYLNDGWVEDDGGQLRLYVGGGASVDVLPQGGRLVAFLSDKFEH
                     EVLPARRERMSFTGWFRRRPLEGFV"
     gene            6281..6778
                     /locus_tag="VEW08_02945"
     CDS             6281..6778
                     /locus_tag="VEW08_02945"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HYJ39719.1"
                     /translation="MSGPLVTLIVAVADNGVIGRGNALPWHLPEDLKRFKRLTMGKPM
                     IMGRKTFESIGKPLPGRLNIVVTRDTNYRHDGVEVVHGTDQALGAAAGAPEVMVIGGA
                     ELFRVFLPRAGRIHLTRVHGNIEGDVVWPALDMRQWEVIERERHEADDRHAHAMTFEV
                     WEKRT"
     gene            6930..7526
                     /locus_tag="VEW08_02950"
     CDS             6930..7526
                     /locus_tag="VEW08_02950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HYJ39720.1"
                     /translation="MTEEQKTLYALGMLMSNSLSTFDLKPEELEQVQKGLADGVGGVK
                     GTINAEDYIPKVQELQKARMAVVSAKAAAAGVAFLDKASKEPGVTKTTSGMVIKQTKA
                     GTGATPAATDQVKVHYEGRLIDGKVFDSSIARGEPATFPLNGVIACWTEGVQTMKVGG
                     KAQLICPANLAYGANGSPPTIPPESTLVFDVELLEIVK"
     gene            7721..10261
                     /locus_tag="VEW08_02955"
     CDS             7721..10261
                     /locus_tag="VEW08_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HYJ39721.1"
                     /translation="MAQGRSRAWLAVVLLGCGAAQAATFTGTVFEDANYGGGAGRSLA
                     ASGGTGLPGVVVELYRADNGNWVDSATTNASGVYSMSSGGTGAPMRVRVVNGSVRSAR
                     VGGAGCDTCVPVQTFRTNASTGVAVAVTNRVGGEAPGSSDAMVNAGTSNYSTLTAGGR
                     IPQSITTADPAAGGSTISGIDFGFNFDTVVNTRDFADCGATNSSYPCQGSLRQFVINS
                     NALGGEGSLSQSGNGEIDGSTSFLPSGFESSIFMIPTAGVAVITLATALPPVSGAFSR
                     LDATTQTVNIGNTNPGTLGTGGTVGVDAISLPTFPRPEVQLAAGNTVLILSGNGSAVL
                     GLALQQGYLRLTGTGCLARNNLVGMTATGISSDNSPTTYGIEFAGSNSTVRNNFVTVN
                     NSGIRTQGGGTGSLVTLNEVARPTSGHTATFDGILLVGTVSSIQVTANLTREQRGGGI
                     EVGFGGGASASNITVTNNTVRNNGFATGGSGAASTEPIGMAAYAYAGSNVVFSRNDIR
                     DNAGAGLLVMTANGTTITQNSFSNNLGLSIDLDTRNIDPNGLGAPQGVTINDTDDLDA
                     GPNGLRNYPVIVSASLVGTELSLGGFARPGSAIELYIAQADPTGFGEGLTYLGVLTEG
                     SAADLDGTTGTYGPANINGVAQGTDNTNRFGFRITVPGGVVIGTRLTGTATISGETSE
                     FGGNVVVTGGPTLVVAKTVTSTSDPVNNLTNPKSIPGSVKLYNVRVTNQGSGPVDNNA
                     VVVVDPVPANTRLFVGNLGAPGSGPIAFVNGSPSSALTWTFTALSSLTDDVDFSNDNG
                     ATWTYVPVPDANGYDAAVTTLRLRPKGTMPGNAGGDPFFELRFRVIVN"
BASE COUNT         2211 a         3576 c         3555 g         2150 t
ORIGIN      
        1 agccggcaat gcctctgcgg aaacccgcga agcgttctgc cgcgcagcgg cgcgcaagct
       61 cgcggaaaaa gccgcgccgc tagttagcga cgcgcgccgc ggcggtgcgc acgagatccg
      121 cgagcgtctc caccggcgcg gagcaggtgg atccacggca gacgtaggct cgtgagctcg
      181 cgcccggctt tttgtccaaa atggcggcgt ccaggccttc gatcgtcgcg ggaatagaga
      241 acaccatgcg atgcggcgcg tagagcttgc cgagttcgcg ttgccaggcg cccgcgtccg
      301 agatctcgcg aatgatcacg atttccggcg gggccaggta ttcggccagc gcctcgagca
      361 ggctcatgtg cccgtgcggg aagcgattga tggcgagcca tgcagcgcgc agcgtgcgtt
      421 cggccgcttc gagccagcgc gtctcgccta acaagtaacc tgctcgaatc agcgcacgcg
      481 ccgcgatgcc attgccggcc ggaatcgcgt cgtcactgaa gctcttgctg cgatggatca
      541 gtgcttcgtg atcgtccgac gtgaagaaga agccgccgag ttcggcgtct tcgaagtgtt
      601 cgagcatggc atcgagaatt tgacgaagcc aggcggcatc ctcgttgcgc cagcggagct
      661 ggagcatctc gagcagcgca ttggcgagat aagcgtagtc atcgaggtag gcattcagat
      721 gcgccacacc atctttggca gtggccagca gccggccccc tccaattgaa ccagggcgcc
      781 acaggtgcgt gcgcgtgaag gcgagggcac gttctgcggc ctcggcaaac tcaggctttt
      841 cgagcgcgcg cgcagcgatg gcgagtccgc gaatcgccag cgcgttccag ctggtgagca
      901 ctttgtcgtc gaggccgggc cacacgcgtt ttgcgcggat cgccagcagc ctggcgcgcg
      961 cgctgtcgac gaacttctcg acttcggacg gctcgaggga aagctccttc ccgatctgct
     1021 cgatggacac gaatacgtgc gcgtgccaca cgccttcgaa attcggttct tcgtcgaggc
     1081 cgaagcgccg gctgaacacg ctgtattcga tgggcgagag cgccgcgcgc acttcggtgc
     1141 gcgtccacac gtagaacttg ccttcgtgac cttcggagtc cgcgtcgtag gccgagtaga
     1201 agcccccgtc ctcgtcctgc atctcgcgca gcatccacgc gccggtttca ttggcgatgc
     1261 gtttgaacag cggatcgcca gtcgccagcg ccgcttcggc gtaggtggcg agcagcgcac
     1321 cgttgtcgta cagcatcttt tcgaaatgcg gaatcatcca gaactggtcg accgagtaac
     1381 gcgcgaagcc gccgcccaac tggtcgtaca tgccgccttc gcccatgcgc gtgagcgtga
     1441 gcgtcgccat gtacaacgcg tgcaggtccg gcgcctgcga tacggaggtg gaatgccagt
     1501 ggtgcatcag ccggtcgatg gaagcggggt gcggaaactt cggagcgtca ccgaacccgc
     1561 cgtaacgctt gtcgaaggtg gcgttcaatt gttcacgtgc ggccatgagc ggcaccgcgg
     1621 tcagtgactc gctggcatcg acgggcgctg gcgtgagatc gccgaatacg tccatgagtg
     1681 tgtcgttttg cgcccgcaat tcgtcgcgct ggtcgcggta gtactccgat acacgcttga
     1741 gcagcgtgga gaaggccggc attccgtacc gggacttgtc aggaaaatac gtgccgccga
     1801 aaaaaggttt ctggtcgtcg tgagtgagga acatcgtcaa cggccagccg ccaccacgct
     1861 gcgtgagcac ctggtgggcg aactggtaga tcttgtcgat gtcgggccgc tcttcgcgat
     1921 ccaccttgat gttgacgaac agctcgttca tcagcgccgc cgtggccgtg tcctcgaacg
     1981 actcgtgggc catgacgtga caccagtggc aggcggagta gccgatggag agcagcaccg
     2041 gtttgccgct gtcgcgcgcc gcctcgaacg cctccggtcc ccaggcgtac cagtgcacgg
     2101 gatttgccgc gtgctgctgc aggtacgggc tcgtttccct tgcaagccga ttcttcggcg
     2161 cgtcgcggtt ctccataggc cgctagaata cagcccgtga ctacccccgt cctcaaactc
     2221 aagcgcggcg aagaccgtcg cctgcgcgcc ggccacctgt gggtcttcag caatgaagtc
     2281 gacactgccg ccactccgct gacccaattc gagcctggcg ctgccgtgca ggtgcattcg
     2341 gacaaggacc agttcctcgg gcacgcctac atcaacccgc gcaccctgat tgcggcgcgt
     2401 attgtcggtc gcgacccgtc ctatccactc gatgcgtcgt tgctggtgca tcggcttcgc
     2461 gtcgcgcttg ccctgcgcga gcggctgtat cgcgaacctt attaccggct ggtgtacggc
     2521 gaatccgacg gactgccggg tctggtgctc gatcgctacg gcgatgtcgt ggttgcccag
     2581 agcggcacgg cgggcatcga ccggctgcgc gccgaggtcg aggacgcggt gaacaaagtg
     2641 attcacccca aggccatcgt gtggaaaaac gattccggtg cgcgtgagct cgaaggcctg
     2701 gagcgaggtg tgtctctggc gggcgaggga gagattccga cggatatttc cgtccgcgag
     2761 cagggcgtcg atttcatcgc gccactggcc gacggccaga agaccggctg gttttacgac
     2821 caggccgcca atcgcgagcg gctgcggcgc tacctgccgg cgggcgcgcg cgtgctcgac
     2881 gtatgcagtt acgtgggcgc ctgggctatc accgcgctca aaggtggcgc ggccagcggt
     2941 acctgcgtgg attcgtcggc gacagcgctc gactatgtct cgcgcaacgc gcgcgcgaat
     3001 ggtgtcgaag tcgaaacgct gaaggacgat gctttcgatg cgctgaaggc gctgcaggaa
     3061 agcggcgcca ggttcgatgt ggtgatcctc gatccgcccg cgttcgccaa gcgcaagaaa
     3121 gacgtacacc aggcgcaagc cgcctatcgc aagctgaacc agctcgcgct gccgctgatc
     3181 gatcgcgacg gcctgctggt gtcgtgctcg tgctcgtatc acatggaggc cgacgagctc
     3241 gtcaatgcaa tccagacagc ggcgcgccac accagccgtt tcgtgcaggt gctggagcag
     3301 ggcggccagt cgcctgatca ccctgtgcat ccggcaatac ccgaaactcg ctatctgaaa
     3361 tcttttttct gccgcgtcac ccgggaataa ttagactacg cgccaatgat tccgtatccc
     3421 tccttcgaca aaattgcctt cgaaatcggc ccgtttggca gtttcggacc cctcaaagtt
     3481 cactggtacg gaatcatgta tctcgtcgcg ttcgcggcgg gctggttcct ggcgcgcttg
     3541 cgcgcaaaaa agccaggctc cacgtggaag cctgtggacg tcgacgattt cctgttcttc
     3601 tgcatgctcg gcgtcattct cggcggccgc ctgggctacg tgtttttcta cgggatgtct
     3661 tactgggcgc tcgatccctg gtatccgttg aagatcgccg acggcggaat gtcgtttcat
     3721 ggcggcctca tcggcgtgat ggtcgcgctc gcggtttttg ccgttcggcg caagcgccgc
     3781 attgccgacg tcttcgattt cgcagcacca cttcccggca ttggcctgtg cgcggggcgc
     3841 atcggcaatt tcatcaacgg tgagctttgg ggtaaaccca ccgacgctcc ctggggattc
     3901 atggtcaatg gcgaaggacg gcacgcgacg cagctctacg aagcggcgct cgaaggtgtc
     3961 gtgctgttcc tcatcatgtg gtggtacacc tccaggccga ggccgcgcat ggcgcccgcg
     4021 ggtctgtttc tcatccttta ttcgctggcg cgcatcaccg tggagttctg gcgtctgccc
     4081 gactcgcact tgaacgaagc cagcggcggc tacctggtcg gccactggtt cacgatgggc
     4141 atgctgctgt cgttgccaat gctcttgatc ggcgccacgc tcatgttcat ggcctaccgg
     4201 cggcgtgaga cttcaggaaa tttctccgcg gtgccggcct gaggtcatga agcagtacct
     4261 tgatttcatg cgacacgtgc gccggaacgg cgcacaaaag gccgatcgca cgggaaccgg
     4321 cacgctctcg gtgttcggac accagatgcg tttcgacctc gccgccggat ttccgctggt
     4381 caccaccaag cgcgtgcact tcaagtccat cgcctacgaa ctgctgtggt tcctgcgcgg
     4441 cgagaccaac acgaaatact tgcgcgagca cggcgtcagc atctgggacg agtgggccga
     4501 tgccaacggc gagctgggcc cggtctacgg caagcaatgg cgttcgtggc cggcacgcga
     4561 tggtcgccac atcgaccaga tcgcgcaggt cgtggagcaa ttgcgcacca gtccggattc
     4621 acgtcgcatc atcgtcagcg cctggaacgt gggtgagctc gaccagatga agctcatgcc
     4681 ctgccacgca ttctttcagt tctacgtgtc ggacaagcgc ctgagttgcc agctctatca
     4741 acgcagcgcg gacattttcc tgggcgtgcc attcaatatt gcgtcctatg cattgctcac
     4801 ccacatgctc gcgcagcagt gcgacctcga ggtgggcgag ttcatctgga ctggcggcga
     4861 ctgccatctc tattcgaatc atctggagca ggtggacgag cagctctcgc gcgttccgct
     4921 caccctgccc aagctggtgc tgacgcgccg gccaccgtcc atgtccgact acgagttcga
     4981 ggattttcag atcgtgaact accagcacca cccggccatc aaggcgcccg tcgccgtatg
     5041 aagaagaaga aaaaagtgaa acctttcgat cagtcgcagc ccaagcgcgg cggcccgccg
     5101 attccgacat cgccgctcgt cgaagtgcgc aattccgacg tccatgggcg cggagtgttc
     5161 gccgtcgctc cgattgccaa gggcacgcgc attttcgagt atctcggtga ccgcgtttcg
     5221 catgccgccg ccgatttgcg ttatgaagat cacgacgaga acgacaatca caccttcctg
     5281 ttcatcgtcg accggaacac cgtcatcgat gcggccgtga acggcagcga tgcacgcttc
     5341 atcaatcacc actgcgatgg caattgcacc tcggtcatcg agcaccgccg cgtgttcatc
     5401 gactcgctgc gcgccatcgc gccgggtgaa gaactgggtt acgactacga gatcggccgt
     5461 gacaaggacg atccgccgaa cgtggatgag atttacgcct gccgatgcgg ttcgccgaag
     5521 tgccgcggct ccatgctgga ggctgctccg aagaagaagg cggccaggcc caagccgaag
     5581 cccaaggcca aacggcgcaa gcccgagaag gctcgccaaa gaacgcgcca aagcacgcac
     5641 aaaggccggc gcgccgagac gaagcgccgc ggagaacggc cacgccggcg cgcctgatcg
     5701 atcagctcgc cgaacgcggt ttcggtgtgg ctgacgattt cgtcgacgcg aagctgctcg
     5761 cagaattgcg cgcgcgctgc atcgagctgc accagacggg aggtttgcgg ccagcgcgtg
     5821 tcggtcgtgg cgacaacgcg cagctcgtcc ccgaagtgcg cggtgacttc atttcgtggc
     5881 tggaaacacc cgagcgcgat gccgagcaac gtgtgctcac ccatctcgat gagctgcgtt
     5941 ccacgctcaa tcgcgcgctc atgaccggtc tcgaagactt ccaggggcac ttcgcgctgt
     6001 atccgagcgg tgccggctac gcacggcatt tcgaccggct ggtgggcaac gacgtgcgcg
     6061 ccatttccgc tgccctgtac ctgaatgacg gttgggtgga agacgacggc ggacagttgc
     6121 gtctctacgt gggtggagga gccagcgtcg acgtgttgcc gcagggcggc aggctggtcg
     6181 cgttcctgtc cgacaagttc gaacacgaag tacttcccgc gcggcgcgaa cgcatgagtt
     6241 tcaccggctg gttccggcgc cggccgctcg agggcttcgt gtgagcgggc ccctcgtcac
     6301 tctcatcgtc gccgtcgccg acaacggagt gatcggccgc ggcaatgcct tgccctggca
     6361 cctgcccgag gatctcaagc gcttcaagcg cctgaccatg ggcaagccga tgatcatggg
     6421 ccgcaagacc ttcgagtcca tcggcaagcc attgcccgga aggctgaata tcgtcgtcac
     6481 acgggatact aactaccgtc atgatggcgt agaggtggtg cacggcacgg accaggcgct
     6541 cggggccgcc gcgggcgcac ccgaggtcat ggtcatcggg ggtgccgagc tgtttcgagt
     6601 ctttctgccg cgcgccggcc gcatccacct gacccgggtg cacggcaaca tcgaggggga
     6661 tgtagtgtgg cctgcgctgg acatgcgcca atgggaagtc atcgagcgtg aacggcacga
     6721 ggcggacgat cgacatgcgc acgcaatgac attcgaggtg tgggagaagc ggacgtagtg
     6781 ggtgaaaaac ctgcgcgaaa ggccctccag cgcgttattc tccccgccct atgaaaaagc
     6841 taactgccat tgcggcactg ttttgcacct tgtccctcac cgtttgcgac gccgatgcgg
     6901 cgaaaaccaa ggcgaaggaa acggccccca tgaccgaaga acaaaagacg ctgtatgcgc
     6961 tcggcatgtt gatgagcaat tcgctcagca cattcgacct caagcccgaa gagctcgagc
     7021 aggtacagaa aggcctggcc gacggcgtgg gcggcgtgaa gggcacgatc aacgcagaag
     7081 actacattcc gaaagtgcag gagctgcaga aagcgcgcat ggcggtagtc tccgcgaagg
     7141 ccgcggcagc tggcgtcgct ttcctcgaca aggcgtcgaa agagccgggc gtcaccaaga
     7201 ccacgagcgg catggtcatc aagcagacca aggcgggcac cggcgcaacg cctgccgcca
     7261 ccgaccaggt gaaggtgcat tacgaaggcc gcctcatcga cggcaaggtt ttcgatagtt
     7321 cgatcgcgcg tggcgaaccg gcgacgttcc cgttgaacgg cgtcatcgcc tgctggaccg
     7381 aaggtgtgca gaccatgaag gtgggcggca aggcgcagct catctgcccg gcgaatctcg
     7441 cctatggcgc caatggttca ccgcccacca tcccgccgga gtccactctg gtgttcgacg
     7501 tggaactgct cgaaatcgtg aagtagttcg acctcgtcaa ttcgtgaaaa caaaggcgcg
     7561 ggagtgcatg ctcccgcgcc tttttgtttg gccgttgcga tccaaatccg cgcgccgatg
     7621 cacgtttctg gccgccaaac tgtgatccat taagggaaag ccccagacgc cggcaattag
     7681 actggccggc atgctagagc ggtcgaatgc aataagagga gtggcgcagg gccgctcgcg
     7741 agcctggctg gccgtcgtgc tgctcggatg cggagccgcg caggcggcca cgttcaccgg
     7801 caccgtattc gaggatgcca actacggcgg tggtgcggga cgctcgctcg ccgcgtccgg
     7861 cggcaccgga ctgccgggcg ttgtcgtgga gttgtatcgc gccgataacg gcaactgggt
     7921 cgactcggcg accaccaacg ccagtggcgt ctattcgatg agctcgggtg gcacgggcgc
     7981 gccaatgcgc gtgcgcgtgg tgaatggctc ggttcgatcg gcccgagtcg gaggagcagg
     8041 ttgtgacacc tgcgtgcccg tgcagacctt ccgcaccaat gccagcaccg gcgtcgccgt
     8101 ggccgtcaca aaccgggtcg gcggtgaagc gccggggtcg agcgatgcga tggtgaacgc
     8161 cggtacgtcg aactactcga cgctgactgc gggtggacgc attccacaat ccatcacgac
     8221 cgcagacccc gcggcgggcg gctcgacaat cagcggcatc gatttcggat tcaacttcga
     8281 caccgtggtg aacacgcgcg acttcgccga ctgcggcgcc accaattcca gctatccatg
     8341 ccagggatca ctgcgacagt tcgtcatcaa ttccaatgca ctgggcggcg agggttcgct
     8401 gagccagtcg ggcaacggcg agatcgatgg atccacatcg ttcctgccca gcggattcga
     8461 atccagcatc ttcatgattc cgacggcggg cgttgcggtc atcacgctgg ccaccgcttt
     8521 gcccccggtt tcgggggcgt tctcgcgcct cgatgcgacg acccagactg tcaacatcgg
     8581 caacaccaac cccggcacgc tgggaaccgg cggcaccgtc ggcgtggatg cgatttccct
     8641 gcccacgttc ccgcgtcccg aagtgcagct cgccgccggc aatacggtct tgatactcag
     8701 cggcaacggc agcgccgtac tcgggctggc gctgcagcag ggttatctgc ggctgacggg
     8761 caccggatgc ctcgcgcgca ataatctcgt tggcatgacc gccaccggta tcagcagcga
     8821 caacagcccg actacctacg gcatcgagtt cgccggcagc aattccaccg tgcgcaacaa
     8881 cttcgtcacc gtgaacaatt ccggcatccg tactcagggc ggcggcaccg gttcgctggt
     8941 cacgttgaac gaagtcgctc gtccgacctc gggccacacc gccacctttg acggcatcct
     9001 gctcgtgggc acggtgagct ccatccaggt gactgcgaat ctcacgcgcg agcagcgcgg
     9061 cggcggcatc gaagtgggat tcggtggtgg cgcctcggcc agcaatatca ccgtgaccaa
     9121 caacaccgtg cgcaacaacg gttttgcgac cggaggcagc ggcgcggcgt ccaccgagcc
     9181 catcggcatg gccgcatacg cctatgccgg ctcgaacgtg gtgttcagcc gcaacgacat
     9241 ccgcgataac gccggcgcag gcctgctggt catgaccgcg aatggcacca cgatcacgca
     9301 gaacagtttc agcaacaacc tcggcctgag catcgatctc gatacgcgga atatcgatcc
     9361 caacggtctc ggagcgccgc aaggcgtgac catcaacgac accgacgatc tggacgcggg
     9421 gcccaacgga ctgcgaaatt atccggtcat cgtctcggcg agcctggtcg ggaccgaact
     9481 atcgctcggt ggcttcgcgc ggcccggcag cgccatcgag ttgtacatcg cacaggcaga
     9541 tcccacgggc tttggcgaag gcctgaccta cctgggcgtg ctcaccgaag gatccgcggc
     9601 cgatctcgac ggcaccaccg gcacctacgg cccggccaac atcaacggcg tcgcgcaggg
     9661 taccgacaac accaatcgat tcggctttcg catcaccgtg cctggcggcg ttgtcatcgg
     9721 cacgcggttg actggtaccg caacgatctc gggcgagact tccgaattcg gcggcaatgt
     9781 cgtggtgacc ggcgggccga cgctggtggt ggcgaagacc gtgacgtcga cgtccgatcc
     9841 cgtcaataac ctcaccaatc cgaagagcat cccggggtcc gtgaagctct acaacgtccg
     9901 cgtcaccaac cagggcagcg gtcccgtcga caacaacgcc gtcgtggtcg tggatccggt
     9961 tcccgccaac acgcgattgt ttgttggaaa tctcggcgct ccgggttcgg ggccgatcgc
    10021 gttcgtcaat ggatcaccga gcagtgcgct gacctggacg ttcaccgcgt tgagcagtct
    10081 caccgacgat gtggatttct ccaacgacaa cggcgccacg tggacctatg tgcccgtgcc
    10141 ggatgcgaat ggttatgacg ccgcggttac gacgctacgc ctgagaccga aaggcaccat
    10201 gcctggcaat gcgggtggcg atccgttctt cgagctgcga ttccgcgtga tcgtcaactg
    10261 atctactccg acgcgacaac caggttcacc tttcctcgac aaatgggaag tggatagtcg
    10321 aacgaatctc taggtgaacg gctgtgcaaa cataaaaaca caaaggctac gctcgaaggt
    10381 tctgaatcag aatgccggtg actgggatag ccaatattga tgttgcaata tacgcataca
    10441 ctagggcggg cctaagaggt gcgccgccag cgattcgcgt acgctctttt tccaaatttc
    10501 cgttgtaaat ccaacaataa tattgcgcgg agccaattgc gaacatgctg agcacgaccg
    10561 ctacaacggt agggacgcca tgcttcgcca gccaagtgaa gtagccggtg aagggatcag
    10621 ttagcatcgt cacgacaatc agattgagac acgtcaagat agtggtagtt atcaaagcac
    10681 cgaacactgg ctgcaaatca ttttttttca acatgtttgc atataggaag tgaaacgttt
    10741 cgcgtagggg attcatctgg cgagacctag taaggtgggt tgagattctc gtagccgttc
    10801 aagcacccca agaaggtcat ggaacgaaca taaggtttca tgcctttgac catcccaagg
    10861 gccggtagag ccacgggcgt cgttgtcggc gcccaaacca tcacaccgcc aagagtagcc
    10921 gacgccgcat cgagcgttcc ctggaggttg tctccattcg cgaagctaac gccggagttt
    10981 atactgtcaa ggaccactcc agctccagca actcctctac cgaatacatg taacgtttgg
    11041 ctggacgtgg cgagcatcga aagacttgcg tatttgctca gaaccgtcgg cgccattgga
    11101 ccaagcccgg tgttaattgg agctgtcgcg ctgagaattg gtgccccgcg agtatgactt
    11161 agagcatttc taaatccatc ggccaacccg cgcgtgagat tgccagccaa gtctgctacc
    11221 aaagcaccgt ccgcgcggtg ttcaatgtcg tcatgcccct caaaaggctc gtcaaacatc
    11281 gtgtccgaaa tcaattgaca ctctgtgctc cgcggcatct ccggcattgg caaacgtcca
    11341 ctataaatgc catcaatcca gaaactggag ctgccccgct tcggtggaca gtttgtcgtc
    11401 ttagtttctt gatgccttct ttcgcttcac ctccagatct tacaacgaag gggagtctgc
    11461 cgccgcaggc ggcagaagct ccgggttcgg at
//
LOCUS       DASQJS010000075        14254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2242023, whole genome shotgun sequence.
ACCESSION   DASQJS010000075 DASQJS010000000
VERSION     DASQJS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14254
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242023"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..612)
                     /locus_tag="VEW08_02960"
     CDS             complement(124..612)
                     /locus_tag="VEW08_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HYJ39722.1"
                     /translation="MPEAERYIAHHRQRFDPSARRNVPAHVTILYPFMAPPLVDDEVV
                     ATITGIARSVPCFNYRLARTERFPVALYLAPDPGEPFSTLMEGIFRAFPDYPPFEGKF
                     ETVVPHVTVAHGDEPLLCEIEVELRIALPGAGVQARCSELVLIENTSGRWEQMHVFAL
                     GA"
     gene            complement(632..880)
                     /locus_tag="VEW08_02965"
     CDS             complement(632..880)
                     /locus_tag="VEW08_02965"
                     /inference="COORDINATES: protein motif:HMM:NF018599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HYJ39723.1"
                     /translation="MLQLRPNCECCNTDLAGDAGDAYICSFECTFCARCTSEKLRGKC
                     PNCSGELVRRPPRAPDKLLKYPASTERVYKPEGCGRVA"
     gene            complement(880..1401)
                     /locus_tag="VEW08_02970"
     CDS             complement(880..1401)
                     /locus_tag="VEW08_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39724.1"
                     /translation="MHPITAMKRLVFAACAASLAAVATAAPSWCLGMSGLGPIRAGMT
                     VDQVMRLADFSGLERKNPAGECWYLHYRGGSSAFDLMIIGGNVVRLELKGGSKLHTLS
                     GARIGTTEDELKAMYGSRLDIQPHKYDTNGHTITVKSSGGDHGLRFETSGGSVQAIQA
                     GPWEHLNYVEGCG"
     gene            complement(1422..2156)
                     /locus_tag="VEW08_02975"
     CDS             complement(1422..2156)
                     /locus_tag="VEW08_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYJ39725.1"
                     /translation="MRILNPRTASEAAVVMALLRAHEIPALLHNGHIASVLPGVMIGG
                     YNSQSVMVPEECAADALELVVNFRTAPSPAGDSSVARNALELLFFSWFLPAPHIPRRG
                     RQLLARFVTLHEVPETECAPIGEPTFAVVLARSANGVVLVFNRHRKVWELPGGLIDPG
                     ESARASAARELAEEAGCIARNLQWLGLVEVADGATHFGAVFACEVDAVPESFASDEIE
                     AITLCRRGSRPRPLGECDAALLNRFG"
     gene            complement(2184..3566)
                     /gene="glmU"
                     /locus_tag="VEW08_02980"
     CDS             complement(2184..3566)
                     /gene="glmU"
                     /locus_tag="VEW08_02980"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HYJ39726.1"
                     /translation="MKLAVVILAAGQGKRMKSDLPKVLQPLAGVPLLGHVVSRARTLN
                     PSSMHVVYGHGGEAVRAALAKEKLPNGDLKWALQAEQLGTGHAVMQAMPNVADEELVL
                     VLYGDVPLIRAETLRQLIDAAGAKAMSLLTVMLDNPTGYGRIVRNSRGAIQKIVEQKD
                     ASKQQLKIREGNSGIMAVPAKLLRKWLGKLRNSNAQGEYYLTDVIAMAVKDKVKVTPL
                     IAPTVAEVLGVNDKAQLAELEGLHRADRARELMVAGATLADPARIDVRGEVSLGRDVF
                     IDANVLLEGKVALGDRVRIGPNTVLRDVSIGADTVVHPNSVLEQSELGAGCIIGPFAR
                     VRPGSKLGAGVHIGNFVELKKTQIADGSKANHLTYLGDAIIGKGVNVGAGTITCNYDG
                     VNKSTTTIDDGAFIGSGNMLVAPVRIGKNATTGAGSTITKDAPDGQLTLARGKQMTLE
                     GWKRPEKKQK"
     gene            complement(3619..3840)
                     /locus_tag="VEW08_02985"
     CDS             complement(3619..3840)
                     /locus_tag="VEW08_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39727.1"
                     /translation="MSPGNDNQVDWTAASTPGEGVVPSLSNGAKPKPRVLPTTKRGVM
                     DTLLGENNLSAHDAGGNDPYNATGRQFRR"
     gene            complement(4002..4544)
                     /locus_tag="VEW08_02990"
     CDS             complement(4002..4544)
                     /locus_tag="VEW08_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HYJ39728.1"
                     /translation="MSRYVALFRGINVGKAKRIAMADLRVLLGKLGYTDVATLLNSGN
                     AVFTGAAEPAGKHAERIRQAVLKKTAVDALVIVKSAKDMAGIIKGNELGAIADDHSRL
                     LVAMTSDEKALATVKALANADWGAEKLHVGKHAAYLWCANGILESKAAEALLKGLKGT
                     GTTRNWATLNKIHALMGKGE"
     gene            complement(4548..4988)
                     /locus_tag="VEW08_02995"
     CDS             complement(4548..4988)
                     /locus_tag="VEW08_02995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ39729.1"
                     /translation="MKHSGIGLASSKEEIDSCFPVMAELRPHLSRVDFSATIQRLMAT
                     ANFSLACLDDGGIKAVAGFRVSEWLHAGKYLEIEDLVTVNGARSKGYGGRLFDWLVSE
                     AARQSCRQVRLLSGVKRTDAHRFYLRKGMSIEAHYFSLNVPAAI"
     gene            complement(4985..5287)
                     /locus_tag="VEW08_03000"
     CDS             complement(4985..5287)
                     /locus_tag="VEW08_03000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HYJ39730.1"
                     /translation="MFGLIGKMTAVPGKREELLWILLGGIGEMPGCLSYVVARDPANA
                     DAIWITEVWESEASHKASLSLSAVQTSIKSAMPLIATFNEHIRTVPVGGHGIKVPG"
     gene            complement(5275..5928)
                     /locus_tag="VEW08_03005"
     CDS             complement(5275..5928)
                     /locus_tag="VEW08_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HYJ39731.1"
                     /translation="MSEIPAGRFSDWLRQMRAALAGSSGMDVACGDCRGCCTSSYFIK
                     VRAHEHAALEHIGEENLRPVPGATNGAQLMGFDEQGHCFMFANGTCSIYAHRPETCRT
                     YDCRVFAAAAMNAGPDKSEINERIASWRFEYPTARDRQEHRAVTAAANFIRQHVIRFP
                     GGRVPSRPSEVAVLAVKSYQVFLDPPVSDAETAAAIIAASRDFDRQTHKQEKAECSD"
     gene            complement(5925..6269)
                     /locus_tag="VEW08_03010"
     CDS             complement(5925..6269)
                     /locus_tag="VEW08_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ39732.1"
                     /translation="MKVNHLHLMVPDVPAASAFFEKYFELRKANGNAGLTVLLDDDGF
                     VLTLMKTGSRSSKTYPENFHVGFFLDSEARVDAIHERMSADGFDVAAPQRTHAYSFYV
                     KAPGGFLVEVGA"
     gene            complement(6327..6722)
                     /locus_tag="VEW08_03015"
     CDS             complement(6327..6722)
                     /locus_tag="VEW08_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39733.1"
                     /translation="MNTLKNFEQVGRRGFFRPVGMMSFEQAIAVVAEGIRHARSLELV
                     DLLVNTSGLSGFPSPDVFARYSLASKWAESAGAALRVAIVARAELIDPQKIGVLMAQN
                     RGVNGDVFSTESAAIAWLDLGTGSTPPTG"
     gene            complement(6827..7252)
                     /locus_tag="VEW08_03020"
     CDS             complement(6827..7252)
                     /locus_tag="VEW08_03020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF001847.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HYJ39734.1"
                     /translation="MATANTIHVDIVSAEGEIFSGAATMVFAPGSQGELGIAPRHAPL
                     LTLLKAGEVRVQTPDGQEQHFFVGGGALEIQPNKVTVLADTALRAKDLDEAAALSAKQ
                     RAEDALRGSIDKLEQAETLRELTRMNEQLRMIQKLRKGK"
     gene            complement(7264..8685)
                     /gene="atpD"
                     /locus_tag="VEW08_03025"
     CDS             complement(7264..8685)
                     /gene="atpD"
                     /locus_tag="VEW08_03025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010117196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HYJ39735.1"
                     /translation="MAQGKITQIIGAVIDVEFPRDSIPKVYEALKLKDVDLTLEVQQQ
                     LGDGVVRTIAMGASEGLKRGLAVETTGGPITVPVGAATLGRIMDVLGAPQDNRGPVDS
                     KDRLPIHRPAPSFAEQTGGQELLVTGIKVMDLLMPFAKGGKVGLFGGAGVGKTVNMLE
                     LINNIAKQYSGLSVFAGVGERTREGNDFYHEMIESGVIDKDTLSNSKVAMVYGQMNEP
                     PGNRLRVALSGLTMAEYFRDEEQGNTGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPS
                     AVGYQPTLAEEMGVLQERITSTKKGSITSIQAVYVPADDLTDPSPATTFAHLDATIVL
                     SRQIAALGIYPAVDPLDSSSRQLDPLVVGEEHYNTARGVQAVLQRYKELQDIIAILGM
                     DELSEEDKKLVFRARKVQRFLSQPFNVAKIFTGQDGKIVSLADTIKGFKGILAGEYDH
                     LPEQAFYMVGNIEEAVEKAKTLN"
     gene            complement(8701..9564)
                     /gene="atpG"
                     /locus_tag="VEW08_03030"
     CDS             complement(8701..9564)
                     /gene="atpG"
                     /locus_tag="VEW08_03030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HYJ39736.1"
                     /translation="MPGTKEIRVKIASVKSTQKITKAMQMVATSKMRRAQERMRMARP
                     YAAKIRAVIGNLTQANPDYRNPFLEERSEVKHVGIIVISSDRGLCGSLNATTFKAALM
                     AMRDWQAKGAGISMSVIGSKATAFFRRLPNPVLSQIQGIGDFPRVSQLVGTIKVMLDA
                     YREGKLDRLLLVHTTFVNTMTQKPEVLQLLPVEPMADAGLQEHWDYIYEPEASDILDA
                     VLRRYIEAQVYRGLVENVASEMAARMVAMKAATDNAGKLIGELQLVYNKARQAAITKE
                     LAEIVGGAAAV"
     gene            complement(9577..11115)
                     /gene="atpA"
                     /locus_tag="VEW08_03035"
     CDS             complement(9577..11115)
                     /gene="atpA"
                     /locus_tag="VEW08_03035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HYJ39737.1"
                     /translation="MSVKASEISDLIKSRIENFKSASEARNVGTVVTVTDGICRIHGL
                     ADVRAGEMIEFPGNLFGLALNLEQDSVGAVVLGDYKGIREGDTVKTTGRILEVPVGRE
                     LLGRVVDALGQPIDGKGPIKATKAAPIERVAPGVIYRKSVGQPVQTGYKAVDSMVPIG
                     RGQRELIIGDRQTGKTAMAIDTIINQKSSGIKCVYVAIGQKQSTIANVVRKLEEHGAL
                     AHTIVVAASASTPAAMQYISAYSGCTMGEYFMDNGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERSARVNEEYVEQATKGAVKGKTGSLTGLPIIETQA
                     GDVTAFVPTNVISITDGQIFLETDLFNAGVRPAMNAGISVSRVGGAAQTDIIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLDRGVRVTELMKQKQYAPMSVAEMALSIYA
                     VNNGYFDKVDSKKVVDVETALQNFARSSHKATIDAVNAEPVLKKHEAALKAICDEFVK
                     NNVA"
     gene            complement(11131..11667)
                     /locus_tag="VEW08_03040"
     CDS             complement(11131..11667)
                     /locus_tag="VEW08_03040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HYJ39738.1"
                     /translation="MAERITTARPYAKAIFSLARTGNTLAATSAGLMRAAEVVTDARV
                     KGLIGSPQVTPAQLAEIVTGVVGAGLDENSRNFVSLLAANRRLGFLPEIAALFELMKA
                     ELENAVDVEVIAASPLTPDQESRYAAALQKKLGRSVRLHTKVDGSLIGGAVLKAGDLV
                     IDGSIKGRLERLAVEMTA"
     gene            complement(11667..12137)
                     /locus_tag="VEW08_03045"
     CDS             complement(11667..12137)
                     /locus_tag="VEW08_03045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004411.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HYJ39739.1"
                     /translation="MDITVTLIIQMLVFLTFVWVVMKFIWPIILGAMNEREKKIAAGL
                     AAAEQGQKDLSEAKSRADEVIKEARVRAQTIESQAQTRANQIMEEARKAASLEGEKAL
                     GLAKAQIDLESNRAREQLRGQVVSLAVAGAKRVLEKEIDAKTHGELLDKLAAKL"
     gene            complement(12164..12445)
                     /gene="atpE"
                     /locus_tag="VEW08_03050"
     CDS             complement(12164..12445)
                     /gene="atpE"
                     /locus_tag="VEW08_03050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HYJ39740.1"
                     /translation="MENIAAIAQIQSFTALGVGLIFGLGALGTAIGFGLLGGKFLEGA
                     ARQPELTPTLQVKMFIVAGLLDAVAMIGIGFALFFTFANPFLNQLPGAQ"
     gene            complement(12485..13315)
                     /gene="atpB"
                     /locus_tag="VEW08_03055"
     CDS             complement(12485..13315)
                     /gene="atpB"
                     /locus_tag="VEW08_03055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004773235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HYJ39741.1"
                     /translation="MSAEVEHAADHAQTGSEYIVHHLGNLQYGEGFWTINVDSILFSV
                     LLGLVFVVSFYLAARNATTGVPGKFQSFVETVVDFVDTQVRDTFHGTSKIIAPLALTI
                     FCWILLMNFMDAVPVDLLPLMGQKVGIEHLKVVPTTDLQITLGMSVTVFLIVMFYSFK
                     IKGFGGFTWELLTHPFGKWMMPFNLLLNLIEHLARPVSLGLRLYGNLYAGEMIFLLLA
                     VLGGSFAITSLAGIGGALGQLLLGVVWACFHILVIPLQAFIFMVLTIVYLSQAHETHH
                     "
     gene            complement(13329..13754)
                     /locus_tag="VEW08_03060"
     CDS             complement(13329..13754)
                     /locus_tag="VEW08_03060"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HYJ39742.1"
                     /translation="MVTLELPQARRLAGSVVLGQVVITAVAAIICFAVWGRTPGLSAL
                     VGGGISVIASAVLVLIGFSAPAGAPPETIARKFYMGEGTKLAVTVALFVAVFAMLKVN
                     FAALFGAYIATLFVYWIALANALPPLSGKTGGVAGAAKR"
     gene            complement(13948..>14254)
                     /locus_tag="VEW08_03065"
     CDS             complement(13948..>14254)
                     /locus_tag="VEW08_03065"
                     /inference="COORDINATES: protein motif:HMM:NF039702.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6250 domain-containing protein"
                     /protein_id="HYJ39743.1"
                     /translation="YYVGLGGNRNTTTRFRRYIGDPVQRPLLPAHDLGSTDVLLVPNK
                     KQSVVLIADRNTIEYKRDGVTLFRLEDRSAYTRGWFALRTTQNHLRVERLRIVSLPR"
BASE COUNT         2487 a         4469 c         4527 g         2771 t
ORIGIN      
        1 gagccacggc gttaccgtgc cacgccagaa ccggccgaga ggctcgccca acggaccggt
       61 gtaacgaaga taggcaacct gaacgggctg ccgttcggtg atctcgattc gcatgcgcgg
      121 agtttacgcg ccgagcgcga atacgtgcat ctgctcccag cgtccgctcg tgttttcgat
      181 gagcaccagt tcggagcagc gcgcctgcac acccgcgccc ggcaacgcga tgcgcagctc
      241 cacttcgatc tcgcacagca gcggctcgtc gccgtgcgcg accgtgacgt gcgggacgac
      301 cgtttcgaac ttgccttcga aaggcgggta gtcgggaaat gcgcgaaaga taccctccat
      361 gagcgtggag aaaggctcgc cggggtcggg cgccaggtac agcgccaccg gaaaccgctc
      421 cgttcttgcg aggcgatagt tgaaacacgg caccgaacgc gcgattccgg tgatggtggc
      481 gactacctcg tcgtcgacca gcggcggcgc catgaatgga tagaggatgg tgacgtgcgc
      541 cggcacattt cgccgcgcgg atgggtcgaa ccgctggcgg tgatgcgcga tgtagcgctc
      601 cgcctcaggc acgcggcaaa tgagcgcggt ttcaggcaac gcgcccgcag ccttcgggtt
      661 tgtaaacgcg ctccgtcgac gccgggtact tcagcagttt gtcgggtgcg cgtggcgggc
      721 ggcgcaccag ctcgccactg cagttgggac acttgccccg caacttctcg gaggtgcatc
      781 gcgcgcagaa ggtgcattcg aacgagcaga tgtaggcgtc gcctgcatct ccggcgaggt
      841 cggtgttgca gcactcgcaa ttcggtctca gttgcagcat cagccacatc cttccacata
      901 gttgagatgt tcccagggac cggcctgtat cgcctggacg cttccgcccg aggtctcgaa
      961 gcgcaatccg tgatcgccac ccgaagattt cacggtgatg gtgtgtccgt tggtgtcgta
     1021 cttgtgcggc tggatatcga ggcgcgaccc gtacattgct ttcagctcgt cttcggtggt
     1081 gccgatgcgg gcacccgaga gggtgtgaag tttgctgccg cctttcagtt ccaggcgcac
     1141 gacatttccg ccgatgatca tgagatcgaa ggccgagctt ccaccccgat agtgcaggta
     1201 ccagcattcg ccggcggggt tcttgcgctc gagaccggag aaatcggcga gccgcatcac
     1261 ctggtccacc gtcatgccgg cgcggatggg cccgagcccg ctcattccca ggcaccagct
     1321 cggggccgct gtcgccacgg ccgcgagcga agcggcgcag gccgcgaata ccaggcgctt
     1381 catggctgtg attggatgca tgcgcagcat tgtgccagcc ctcaaccgaa tctgttgagc
     1441 agcgcagcgt cgcactcgcc cagcgggcgg ggacggctgc cgcggcggca cagcgtaata
     1501 gcttctattt catcgctcgc gaaactttct ggcacggcat ccacctcgca ggcgaacacc
     1561 gccccgaaat gcgtagcgcc gtcggccact tccacgaggc cgagccattg gagattgcgc
     1621 gcaatgcacc ccgcttcttc ggcgagctcg cgcgcggcgc tggcgcgcgc cgattcaccg
     1681 gggtcgatga gaccacccgg cagttcccac accttgcggt ggcgattgaa tacgagcacc
     1741 acgccgttcg cgctgcgcgc caggaccacg gcgaaagtgg gctcgccgat cggcgcgcac
     1801 tccgtctccg gcacctcgtg cagcgtgacg aaacgtgcga gcaactggcg gccacggcgc
     1861 ggaatgtgcg gcgccggcag gaaccacgag aaaaacagca gttcgagtgc gttccgcgca
     1921 accgagctat cgccggcggg tgaaggcgcc gtgcggaagt tcacgaccag ctcgagagca
     1981 tccgcggcgc attcttccgg caccatcacg ctttgggagt tgtagccgcc gatcattacg
     2041 cccggcaaca cgctggcgat atggccgttg tgcagaagtg ccgggatctc atgcgcgcgc
     2101 agcagggcca tgacaacggc cgcctcggac gcggttcgag ggttcagaat gcgcaccagc
     2161 cccatggctc gtttcgcctt cttctatttt tgcttttttt ctggccgctt ccagccttcg
     2221 agcgtcatct gcttcccgcg cgccagcgtg agctggccgt cgggcgcgtc cttggtgatg
     2281 gtgctgcccg cgcccgtggt ggcgttcttg ccgatgcgca ccggcgccac cagcatgttg
     2341 ccggagccga tgaaggcgcc gtcgtcgatg gtggtggtcg acttgttcac accatcgtaa
     2401 ttgcaggtga tggtgcccgc gccgacattc acgcctttgc cgatgatggc gtcgccgaga
     2461 tacgtcaggt ggttggcctt gctgccgtcg gcaatctggg tcttcttgag ctccacgaaa
     2521 ttgccgatgt gcacgccggc gccgagcttc gaccccggac gcacgcgtgc aaacggcccg
     2581 atgatgcagc cggcgccaag ctcgctctgc tcgagaacgg aattcggatg gacgacggta
     2641 tctgcgccga tggatacgtc gcgcagcaca gtgttcggac caatgcgcac gcgatcgccc
     2701 agcgcgactt tgccttcgag cagcacattg gcgtcgatga agacatcgcg cccgaggctg
     2761 acttcgccgc ggacatcgat acgtgccgga tccgccagag ttgcacccgc gaccatgagc
     2821 tcgcgcgcgc gatcggcgcg atgcagacct tcgagctcgg ccagctgggc cttgtcgttg
     2881 acgccgagaa cttccgccac cgtgggcgcg atcagcggcg tcaccttcac cttgtctttc
     2941 accgccatgg cgatgacatc ggtgaggtag tactcgccct gcgcattgga gttcctgagc
     3001 ttgccgagcc atttgcgcag tagtttggct ggcacggcca tgatgccgct gttgccttcg
     3061 cgaatcttca gctgctgctt gctggcgtcc ttctgctcga cgattttctg gatggcgccg
     3121 cgcgaattgc gaacgatacg gccatagccg gtgggattgt cgagcatcac ggtgagcagg
     3181 ctcatggcct tcgcaccggc ggcgtcgatg agttgcctca gggtttcggc gcggatcagc
     3241 ggcacgtcac cgtacagcac gagcaccaac tcttcgtcgg cgacatttgg catggcctgc
     3301 atgaccgcgt ggcccgtgcc cagttgctct gcctgcagcg cccatttcag atctccattg
     3361 ggcagctttt ctttcgcgag cgccgcgcgc accgcctcgc cgccatggcc atagaccacg
     3421 tgcatcgacg aaggattgag ggtgcgtgcc cggctaacaa catgcccgag caggggcaca
     3481 ccggcgagcg gctgcaggac cttggggaga tcggacttca ttcgcttgcc ctggccggca
     3541 gcgagaataa cgacggcgag tttcatgaag ccgcaagttt agcggcccgc cgaaaaatag
     3601 acactcgatc gacgcgaatc agcggcggaa ctgcctgccg gttgcgttat agggatcgtt
     3661 gccgcccgca tcgtgggccg agagattgtt ctcacctagc agcgtatcca tgaccccgcg
     3721 cttggtcgtc gggagaacgc gcggcttggg ctttgcgcca ttcgacaggc tcggaacaac
     3781 gccctcgccc ggggttgagg cggcagtcca gtcgacctga ttgtcgtttc cagggctcat
     3841 gggaccgaaa tatacgcgcg gaatctttgc aggggtatgt ccgcggtcgg ctgtccgcgg
     3901 ccgctcgtcg gggcggccgc cggtcatcgg ccgctatcta ccgctcatcg gactactgat
     3961 ttcagcctgg ttttcaccgc cttgcagggc ccgtgtccgg gtcactcgcc cttgcccatg
     4021 agggcgtgaa tcttgttgag cgtggcccag ttgcgcgtgg tgccggtgcc cttgagtcct
     4081 ttcagcaatg cctccgccgc tttgctttcc aggatgccgt ttgcgcacca caggtacgcg
     4141 gcgtgtttgc cgacgtgaag cttctcggca ccccaatcgg cgttcgccaa cgccttgaca
     4201 gtggccaggg ctttttcgtc gctggtcatc gccacgagca ggcgcgagtg atcgtcggca
     4261 atcgcgccga gttcattgcc tttgatgatt ccggccatgt ccttcgcgga cttcacgatg
     4321 accagggcat cgacggcggt ttttttcagc accgcctggc gtattcgttc cgcgtgtttg
     4381 ccggcgggct ctgccgcacc ggtgaaaacc gcattgccgc tgttcagcag cgtcgccacg
     4441 tccgtatacc caagcttgcc gagcagcacg cgcagatcgg ccatggcgat gcgcttggct
     4501 tttccgacat tgatcccacg aaacagagcg acgtagcgcg acatgactca gatggcggcg
     4561 ggcacgttca gggagaaata gtgtgcctcg atgctcatgc ctttgcgcag gtagaagcga
     4621 tgagcatcgg tacgcttgac gccggagagc agccggacct ggcgacagga ctgtcgcgca
     4681 gcttcactta ccagccagtc gaacagccgg ccgccgtagc ctttcgagcg cgcgccattc
     4741 accgtcacca ggtcttcgat ctcgagatac ttcccggcgt gcagccactc agagacgcga
     4801 aagcccgcca ccgccttgat cccgccgtcg tccagacagg cgagcgagaa gtttgccgtc
     4861 gccatcaggc gctggatcgt ggcggagaag tcgacacgcg aaagatgcgg gcgaagttct
     4921 gccatcacgg gaaaacaaga gtcgatttct tccttgctgg atgcgagccc gatcccggag
     4981 tgcttcaacc cggcaccttg atgccgtgcc cgcccacggg cacggtcctg atgtgttcgt
     5041 tgaacgtggc gatgagcggc attgcgctct tgatcgaggt ctgcacggcc gacagcgata
     5101 acgaagcttt gtggctcgct tcgctctccc atacctccgt aatccagatc gcatcagcgt
     5161 tggcgggatc gcgcgccacg acgtagctca ggcagccggg catttcgccg atgcctccga
     5221 gcaatatcca cagcagctct tcgcgcttgc cggggacggc agtcatcttg cctatcaatc
     5281 cgaacattcg gctttctcct gtttatgggt ttgccggtcg aagtcacggc tggcagcgat
     5341 gatagccgcc gcggtctccg cgtccgatac cggtggatcg agaaatacct ggtagctctt
     5401 taccgcaagc accgcgacct cgctgggacg cgaggggacc cggccgcccg gaaagcggat
     5461 cacgtgctgg cggatgaagt tcgcggccgc ggtgaccgcc cgatgctctt gccggtcgcg
     5521 ggcggtgggg tattcgaagc gccagctcgc gatgcgctcg ttgatttcgg acttgtcggg
     5581 gccggcattc atggcagcgg cggcgaacac gcggcagtcg tacgtgcggc aggtttcggg
     5641 acgatgcgcg tagatagagc aggtgccgtt ggcgaacatg aagcaatggc cctgctcgtc
     5701 gaagcccatc agctgagctc cgttcgttgc ccccggcacg gggcgcaggt tttcctcgcc
     5761 gatatgctcg agcgcggcgt gttcatgcgc gcgcaccttg atgaaatagc tggacgtgca
     5821 gcagccgcgg caatcgccgc aggcgacgtc catgcccgaa cttccagcga gcgccgcgcg
     5881 catctggcgc agccagtccg agaaccggcc agccgggatc tcgctcaagc gcccacctcg
     5941 accaggaatc cgcccggcgc tttgacgtag aaggaatacg catgcgtgcg ctggggagcg
     6001 gcgacgtcga agccatcggc actcatgcgt tcgtgaatgg catccaccct ggcttcgctg
     6061 tcgagaaaaa acccgacgtg aaagttctcg ggatacgtct tgctcgacct ggagcccgtc
     6121 ttcatcagcg tcagcacgaa gccgtcatcg tcgagcagca cggtgaggcc ggcgttgccg
     6181 ttcgccttgc gcagttcgaa atacttttcg aagaaggcgc tggccgcggg cacgtccggc
     6241 accatcaggt gcaggtgatt cactttcatg gccgcatgct cgcgcttgcc gcgcgactgc
     6301 gcaatgaagc ccgggttgta aatgcctcag cccgtaggcg gagtcgaccc ggttcccaga
     6361 tcgagccagg cgatggccgc cgattcggtg gaaaagacgt cgccattgac gccgcggttc
     6421 tgcgccatga gcacaccgat tttctgtggg tcgatgagtt cagcccgcgc cacaatggcc
     6481 acgcgcaatg ctgccccggc gctctccgcc catttgctgg ccagcgagta gcgcgcgaaa
     6541 acatccgggc tcgggaagcc cgacaggccg ctggtgttca ccagcagatc caccagttcg
     6601 agtgaacgcg catgccggat tccctcggct acgactgcga tcgcctgctc gaaggacatc
     6661 attcccacgg gtcgaaagaa gccgcgccgg ccaacctgct cgaagttttt aagcgtgttc
     6721 attgcggtcc gtcagactct agcgccacgg cgagcgtgga cgtatcaggg aaacattggg
     6781 gtgtccgcgg cgggacctgc accgcgggcg aatagggctg cgcggactat ttacccttgc
     6841 gcaacttctg gatcatgcgg agctgctcgt tcatgcgggt gagttccctg agggtttccg
     6901 cctgttcgag cttgtcgatg ctgccgcgca gcgcgtcttc ggcgcgttgc ttcgcggaca
     6961 gggccgcggc ctcgtccagg tccttcgcgc gaagcgcggt gtccgcgagc acggtgacct
     7021 tgttgggctg gatttcgaga gcaccgccac cgacgaagaa atgctgctcc tggccgtccg
     7081 gcgtctgcac gcgaacttca ccggccttga gcagcgtgag cagcggcgca tgacgcggtg
     7141 caatgcccag ttcaccctgg ctgccgggcg cgaacaccat ggtcgccgcg cccgaaaaga
     7201 tttctccctc ggcgctgacg atatcgacgt gaatggtgtt tgcggtggcc atgtctatct
     7261 accttagttg agcgtcttcg ccttctcgac ggcttcttcg atgttgccga ccatgtagaa
     7321 cgcctgctcc ggcaggtgat cgtattcgcc ggcgagaatg cccttgaagc ccttgatggt
     7381 gtcggccagc gaaacgatct tgccgtcctg gccggtgaag atcttcgcca cgttgaacgg
     7441 ctgcgacagg aagcgctgca ccttgcgcgc gcgaaacacg agcttcttgt cttcttccga
     7501 cagctcgtcc atcccgagaa tcgcgatgat gtcctgcagt tccttgtaac gctgcagcac
     7561 ggcctgcacg ccgcgtgcgg tgttgtagtg ttcttcgccg acgaccagcg ggtcgagctg
     7621 acggctgctg gagtcgagcg gatcgacggc cgggtaaatg ccgagcgccg cgatctggcg
     7681 cgacagcacg atggtggcgt cgaggtgggc gaacgtcgtc gcgggtgacg gatcggtcaa
     7741 gtcgtccgcg ggcacgtaca ccgcctggat ggaggtgatc gaacccttct tggtcgaggt
     7801 gatgcgttcc tgcagcacgc ccatttcttc ggcgagagtc ggctggtaac ccaccgccga
     7861 aggcatgcgg cctaacaacg cggacacttc ggtgccggcg agcgtgtagc gatagatgtt
     7921 gtcgacgaag aacagcacgt cgcggccctt gccggtgttg ccttgctctt catcgcggaa
     7981 gtattcggcc atcgtgaggc ccgagagcgc gacgcgcagg cggttgcccg gcggctcgtt
     8041 catctggccg tacaccatgg cgaccttcga gttcgagagc gtgtccttgt cgatgacgcc
     8101 cgactcgatc atctcgtgat agaagtcgtt gccttcgcgg gtacgttcac ccacgccggc
     8161 gaacaccgac aagccgctgt actgcttggc gatgttgttg atgagctcga gcatgttcac
     8221 ggtcttgccc acgccggcgc cgccgaacag gccgaccttg ccgcccttgg cgaatggcat
     8281 gagcaagtcc atgaccttga tgccggtgac cagcaattcc tggccgcccg tctgttccgc
     8341 gaacgacggc gcgggccggt ggataggcag gcggtccttg gaatcgacgg gaccacggtt
     8401 gtcctgcggc gctccgagaa cgtccatgat ccggcccagc gtcgccgcgc ccacggggac
     8461 cgtgatcggc ccgccggtgg tttccacggc gagaccgcgc ttgaggcctt cggaggcgcc
     8521 catggcgatc gtgcgcacca cgccgtcgcc gagctgctgc tgcacttcga gcgtcagatc
     8581 gacgtccttg agcttcagcg cttcatagac cttcgggatc gaatcgcgcg ggaactccac
     8641 gtcgatgact gcgccgatga tctgggtaat cttgccttgg gccatgttca aatcctctaa
     8701 ctaaactgct gccgcgccac cgacgatctc agccaattcc ttggtgatcg cggcctggcg
     8761 ggccttgttg taaacgagct ggagttcgcc gatgagcttg ccggcgttgt ccgttgccgc
     8821 cttcatggcg accatgcgcg ccgccatttc gctggcgacg ttttcgacca gcccgcgata
     8881 gacctgcgcc tcgatgtagc gccgcagaac ggcgtcgaga atgtcactgg cctcgggctc
     8941 gtaaatgtag tcccagtgct cctgcagacc ggcatctgcc atcggctcga cgggcagcag
     9001 ctgcagcact tcgggcttct gcgtcatggt gttgacgaag gtcgtgtgca ccagcagcag
     9061 ccggtccagc ttgccctcgc gatacgcgtc gagcatgacc ttgatcgtgc cgacgagctg
     9121 gctgacgcgc gggaaatcgc cgatgccctg gatctgcgag agcaccgggt tcggcagccg
     9181 gcggaagaat gcggtggcct tggagccgat cacgctcatc gagatgccgg cgcccttggc
     9241 ctgccagtcg cgcatcgcca tcagcgccgc cttgaaggtg gtggcgttga gcgagccgca
     9301 cagtccgcga tcggacgaga tcacgatgat gccgacatgc ttcacttcgc tgcgttcctc
     9361 gaggaacggg ttgcgatagt cggggttggc ctgcgtgaga ttgccgatga ccgcgcggat
     9421 cttcgcggca tacggacgcg ccatgcgcat gcgttcctgg gcgcgacgca tcttgctggt
     9481 cgccaccatc tgcatcgcct tcgtgatctt ctgcgtgctc ttgacgctgg cgatcttgac
     9541 gcggatttct tttgttccag gcatctaact tttccgtcag gcgacgttgt tcttcacgaa
     9601 ctcatcgcaa atggccttga gtgcggcctc gtgcttcttg agcacgggct ccgcattgac
     9661 ggcatcgatg gtcgccttgt ggctgctcct ggcaaagttc tgcagggcgg tttccacgtc
     9721 gacgaccttc ttgctgtcga ccttgtcgaa gtagccgttg ttcaccgcgt agatggacag
     9781 cgccatttcc gccaccgaca tcggggcgta ctgcttctgc ttcatcagtt cggtgacgcg
     9841 cacgccgcgg tcgagctgct tgcgggttgc ctcgtcgagg tcggacgcaa actgcgagaa
     9901 cgccgcgagc tcgcggtact gcgcgagggc gaggcgaata ccgccgccca gcttcttgat
     9961 gatgtcggtc tgcgcggcgc cgccgacgcg cgacaccgag atgccggcgt tcatggccgg
    10021 gcgtacgccc gcgttgaaca ggtcggtctc gaggaagatc tggccgtcgg tgatggagat
    10081 gacgttcgtc ggaacgaacg cggtcacgtc accggcctgc gtctcgatga tgggcagacc
    10141 cgtcagcgaa ccggtcttgc ccttcacggc gcccttggtc gcctgctcca cgtactcttc
    10201 gttgacgcgg gcggaacgct cgagaaggcg gctgtgcaaa tagaacacgt cgccggggaa
    10261 cgcttcgcgg cccgggggac ggcgcagcag cagcgaaatc tggcgatagg ccacggcctg
    10321 cttcgacaga tcgtcataga tgatgagcgc gtcttcaccg ttgtccatga agtattcgcc
    10381 catcgtgcag cccgagtacg cgctgatgta ctgcatggcg gccggcgtcg aagcggacgc
    10441 ggcgacgacg atggtgtgcg ccagtgcgcc gtgctcttcg agcttgcgca ccacgttggc
    10501 gatggtcgac tgcttctggc cgatggcgac gtacacgcac ttaatgccgg aagacttctg
    10561 gttgatgatg gtgtcgatgg ccatcgcggt cttgccggtc tggcggtcgc cgatgatgag
    10621 ctcgcgctgg ccgcggccga tcgggaccat ggagtcgacg gccttgtagc cggtctgcac
    10681 cggctggccg acgctcttgc ggtagatgac gcccggcgcc acgcgctcga tgggagcggc
    10741 tttcgtcgcc ttgatcgggc ccttgccgtc gatgggctgg ccgagcgcgt cgaccacgcg
    10801 gcctaacaac tcacggccca ccggcacttc gagaatgcgt cccgtggtct tcacggtgtc
    10861 gccttcgcgg atgcccttgt agtcgccgag caccacggcg cccacggagt cctgctcgag
    10921 gttcagcgcg aggccgaaca ggttgcccgg gaactcgatc atttcgccgg cgcgaacgtc
    10981 cgcaagaccg tggatgcggc agatgccgtc cgtcaccgtg acgacggtgc cgacgttgcg
    11041 cgcttcgctc gcggacttga agttctcgat acgtgacttg atgaggtctg agatttcgga
    11101 tgctttgacg gacatagatc tattcctaac ttaagcagtc atttcaacgg ccaggcgttc
    11161 gagccggccc ttgatggagc cgtcgatcac cagatcgccg gccttgagga cggcgccgcc
    11221 aatgaggctg ccgtcgacct tggtatgcag gcgcacgctg cgcccgagtt tcttctgcaa
    11281 tgcggccgcg tagcggcttt cctggtcggg cgtgagcggc gacgccgcga tcacttcgac
    11341 atcgaccgcg ttctccagct cggccttcat cagttcgaac agcgccgcga tctcggggag
    11401 gaatccgaga cggcggttgg ccgccagtaa cgagacgaag ttgcggctgt tttcatcgag
    11461 ccccgcaccg accacgccag tgacgatttc ggcaagctgc gccggtgtca cctgcgggct
    11521 tccgatcagc ccctttacgc gggcatcggt gaccacttcg gcggcacgca tgagacccgc
    11581 ggacgtcgcc gcgagcgtgt tgccagtgcg cgcgagcgag aaaatcgcct tcgcgtacgg
    11641 acgtgcggtg gtgatgcgtt cagccattag agcttcgcag ccagtttgtc gagaagttcg
    11701 ccgtgcgtct tcgcgtcgat ctctttctcg agcacgcgct tggcgccggc cacggcgagg
    11761 cttactacct ggccgcgcag ctgttccctt gcacgattgc tctcgaggtc gatctgcgcc
    11821 ttggccaggc ccagggcctt ctcgccctcg aggctcgcag ccttgcgcgc ttcttccatg
    11881 atctggttgg cgcgcgtctg tgcctgagac tcgatggtct gtgcacggac gcgggcttcc
    11941 ttgatcactt catcggcgcg gctcttggcc tcggacaggt ccttctggcc ttgctcggcg
    12001 gccgcgagtc cggcggcgat cttcttctcg cgttcattca tcgcgccgag aatgatcggc
    12061 cagataaatt tcatcacgac ccagacgaag gtcaggaaga ccagcatctg gataatgagt
    12121 gtgacggtga tatccacgcg cctctcctag cgagttacaa cgattactgc gcgcccggga
    12181 gctggttcag gaacggattc gcgaacgtga agaacagcgc gaagccgata ccgatcatcg
    12241 cgaccgcgtc gagcaggccg gcgacgatga acatcttcac ctgcagggtc ggggtcagtt
    12301 caggctggcg cgcggcgcct tccaggaact tgccgccgag cagaccgaag ccgatggcgg
    12361 tgccgagcgc gccgaggccg aagatcaggc cgacgccgag agcggtgaaa ctttgaattt
    12421 gggcaatagc agcaatgttt tccatgattc ctccaacaga acttttatct aaacaacatt
    12481 tgaatcagtg atgagtctcg tgcgcctgcg acaggtagac gatcgtcagc accatgaata
    12541 tgaacgcctg cagcgggatg accaggatgt ggaagcaggc ccagaccacg cccagcagga
    12601 gctgacccag ggcgccaccg ataccggcga gcgaagtaat cgcgaaactg ccgccgagca
    12661 cggcgagcag caggaagatc atttcgccgg cgtacaggtt tccgtacaga cgcaggccga
    12721 gcgacacggg ccgcgccagg tgctcgatga ggttcagcaa caggttgaac ggcatcatcc
    12781 acttgccgaa ggggtgcgtg agcagctccc aggtgaagcc gccgaagcct ttgatcttga
    12841 agctgtagaa catgacgatc aggaacaccg tgaccgacat gccgagcgtg atctgcagat
    12901 cggtggtcgg cacgaccttg aggtgttcga tgccgacctt ctggcccatc agcggcaaca
    12961 ggtcgaccgg cacggcgtcc atgaaattca tcagcaggat ccagcagaag atcgtcagcg
    13021 ccagcggcgc gatgatcttg ctggtgccgt ggaacgtgtc gcgaacctgc gtgtccacga
    13081 aatccaccac ggtctcgacg aagctctgga acttgccagg cacaccggtc gtcgcattgc
    13141 gcgcggccag gtagaacgag accacgaaca ccaggccgag cagcacggaa aacaggatgc
    13201 tgtcgacgtt gatggtccag aaaccttcac cgtactggag gttgcccaga tggtgaacga
    13261 tgtattcgct cccggtctgc gcatggtccg cggcgtgttc gacttcggct gacatctatc
    13321 tgagcttcct aacgcttggc ggcgcccgcg acgccaccgg tctttccaga cagcggcggc
    13381 agtgcgttcg caagtgcgat ccagtacacg aacaacgtgg cgatgtaggc gccgaacaac
    13441 gcggcgaaat tcaccttcag catcgcgaac acggccacga acagcgcaac cgtgacggcg
    13501 agcttcgtgc cctcgcccat gtagaacttg cgcgcgatgg tctcgggcgg ggcgcccgca
    13561 ggcgcgctga aaccgatgag gaccagcacc gcgctcgcga tgacggagat gcctccgccc
    13621 acgagggcgg acaaaccagg cgtacgcccc cacaccgcga aacagatgat cgcggccacg
    13681 gcagttatga cgacctggcc aagcactacg cttccggcca gtcgcctcgc ttggggcaac
    13741 tcaagggtga ccacgacact cctattgcaa gtcgacaatc tgcgtacacg accggcccac
    13801 gagggacgca ggctggttca gctgtgttcg aaaattgttt ggcttggcaa cgacttacgt
    13861 gtgccttggc caggggcgcg cattatatac accgacccca ccagcatcaa cacgaaaacg
    13921 cggaaaatct cgcggccgcg agcctggtca gcgcggcagt gaaacgatgc gcagccgctc
    13981 gacgcgcagg tggttctggg tggtgcgcag cgcgaaccag ccccgggtgt atgcggaccg
    14041 gtcctccaat cggaatagcg ttacaccgtc gcgtttgtat tcaatagtgt tccgatcggc
    14101 tatgaggacg acgctttgtt tcttatttgg gacaagcagt acatctgtgg aaccgagatc
    14161 gtgtgcaggc agcagcggtc gctgcacggg atcaccgatg tagcggcgaa atcgcgtggt
    14221 ggtgttccgg ttgccgccca gtcccacgta atag
//
LOCUS       DASQJS010000076         5595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2869989, whole genome shotgun sequence.
ACCESSION   DASQJS010000076 DASQJS010000000
VERSION     DASQJS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2869989"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..445)
                     /locus_tag="VEW08_03070"
     CDS             complement(92..445)
                     /locus_tag="VEW08_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39744.1"
                     /translation="MRPDKSLVPQALRAVKIVHTLAWALFAGCIVALPVAAYLRDFQL
                     AALLVAIVLVEVIVLVANRFRCPLTAVAARYTSDRRANFDIYLPVWIARYNKEIFGTL
                     FVAGILFTLVRWRFA"
     gene            980..1396
                     /locus_tag="VEW08_03075"
     CDS             980..1396
                     /locus_tag="VEW08_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ39745.1"
                     /translation="MKALAVIATIASMMSAALAAENAPPAKVTPVTTRDLEGIAGKEM
                     LVLVVEYPPGGVSQPHRHNASVFVYVLEGAVTMQVQGSAPVTLKPGETFYEAPGDIHV
                     TSANASSSAPAKFLVYMVKDKGAPASAPAPGIAPGG"
     regulatory      1426..1651
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1768..4065
                     /gene="metE"
                     /locus_tag="VEW08_03080"
     CDS             1768..4065
                     /gene="metE"
                     /locus_tag="VEW08_03080"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HYJ39746.1"
                     /translation="MTVIRAINLGFPRVGVKRELKFAEENYWKGAGTREALLATAKDL
                     RLRHWRLQNDAGIDDIPCNDFSLYDQMLDMSCLLGCVPRRFGWQSDVVDLYTYFAMAR
                     GSKTAAAMEMTKWFDTNYHYIVPEFEEGMRFRIGSTKLFDELAEARAVGIEPTPVLIG
                     PVSYLLLGKAKYPNCDLARIAERLLPVYGQILKSLADQSVSRVQIDEPCLVTDLSREA
                     RALYPMAYRALAQAAPLSINLTTYFGDLDDNRHLAFSLPVSSVHVDLARAPEQLEPCI
                     EAALANNKRLSLGVVDGRNVWRNDLSRSLAAVERAAAVLGEERVAVGPSCSLLHSPCD
                     LKEERAMDPGIRSWLAFATQKLDEVAALKRGVTSGRSTIAHELKTSNDVQSARRGSPR
                     IHDSRVAQRVDSVIPPMLNRGRAFAERRKLQASEFKLPLFPTTTIGSFPQTAEIRAAR
                     AAHKAGRLEPQAYEAAMRKEIEGMVRFQERIDLDVLVHGEPERNDMVEYFGEQLEGYV
                     FSENGWVQSYGSRCVKPPIIYGDVSRPQPMTVKWSKFAQSLTPRIMKGMLTGPVTMLQ
                     WSFVRDDQPRAQTCRQIALAIRDEVLDLEAAGIRIIQIDEPAIREGLPLRREQWPAYL
                     RWAVEAFRLSAAGVRDHTQIHTHMCYSEFNDIMDAVAAMDADVISIETSRSHMELLDA
                     FVTFRYPNEIGPGVYDIHSPRVPSASDMGALLDKARGVLKAEQIWVNPDCGLKTRGWP
                     EVERALVNMVSVAKTARETVSGTIS"
     gene            4122..4247
                     /locus_tag="VEW08_03085"
     CDS             4122..4247
                     /locus_tag="VEW08_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39747.1"
                     /translation="MDLPNLLHEYGYLLIFVGVRHALPLGAAHRTAAGTSSTTGK"
     gene            4351..5412
                     /locus_tag="VEW08_03090"
     CDS             4351..5412
                     /locus_tag="VEW08_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HYJ39748.1"
                     /translation="MIPTEPIGSIPRPKELIDGIAALGDGSDPRLEPLYDAAILDTVR
                     QFEATESPVITDGEQRKYHNFWTYSVEGLETTAPDGFRIPFAAGHVRRMPRLVSGPFR
                     YKRYADRFLEQALRHSTVPVKQAVISPSALSLMYPADGIANYSREEFIEDLVNEHVTE
                     VRRCLDMGAHCVQIDFTEGRLAIKIDPTGALLASFIELNNLALSRLTPAERQRVGVHT
                     CPGGDRDSTHSADVPYKELLPSLFQLNVTNFYLSLAGERDREGALGIIAANLKPGHRA
                     FIGVIAPIDPRLESAEIVRDRVLEAARFIPVHQLGTTDDCGFAPFCDDQSTSRELAFA
                     KIRARVAGTAMAENALGER"
     gene            5412..>5595
                     /locus_tag="VEW08_03095"
     CDS             5412..>5595
                     /locus_tag="VEW08_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39749.1"
                     /translation="MATDEEELKIRTAALKNIEAILNARQRAENDLVAAKLTLEKRTE
                     ELSQQREWFEVTLASIG"
BASE COUNT         1106 a         1762 c         1759 g          968 t
ORIGIN      
        1 tcacagacgc gagaaggccc gccaaacacc gaactgctgg cacacaggca gagtacgagg
       61 cctatcttgt ttgcttgcgt gatcgccatt ttcaagcgaa ccgccatcgg acaagagtga
      121 acaggattcc cgccacgaac agggttccaa agatctcctt gttgtagcgg gcgatccata
      181 ccggtagata gatgtcgaaa ttggcgcgtc ggtcgcttgt gtagcgagcc gcgacggcgg
      241 tcaggggaca ccgaaagcgg ttggcgacga gcactattac ctcgacaaga acgatggcga
      301 ccagaagtgc tgcgagctgg aagtccctga ggtacgccgc aaccgggagc gccacaatgc
      361 aacctgcgaa caacgcccag gccagggtgt gcacgatctt cacggcgcgc aaggcctgag
      421 gaaccagcga tttgtcaggc cgcatgatca ttggcgagca ggttcaaggc gaactggctc
      481 acttaggacc actaccgcgg gttgccaatc agtgactcgg acctctgact ccttgaatcc
      541 gtcagccgta atgttcaaga agaataccct caccggttct tgagggatga tgaaccatac
      601 agaccactgc ttgcgttcgg ggagcgtgaa tgagccatct gaaagtgtac tgacttcagt
      661 gtcagcccat ctcgcatccc tgatgtgtgc attctcaatc gggatgttct gagcatctac
      721 cacgtgacct gtaattgtcg ggcgcagcgt gtacttgtat ggaaaacagc cgctcagcaa
      781 gacgagcgtg aaaagcagca acccgtggat cgcagctttc atcatgcggc ctaactatag
      841 attctcggac tgtcggatag cagcgcgacc atcggcaact ccctgaccaa cctggggctt
      901 gcccgatgtt atttcttcat ctggggctac ggcaatgagg gactacaatg ggtcatcatt
      961 caacgggttc ggaggtcgta tgaaagctct tgccgtcatc gcaaccatcg cctcaatgat
     1021 gtccgccgcc ctggcggccg aaaacgcacc tcccgcgaag gtcacgcccg tcacgacccg
     1081 cgatctcgaa ggcatcgccg gcaaggagat gctggtgctg gtcgtggaat atccgccggg
     1141 cggagtctcc cagccgcatc ggcacaacgc gtcggtgttc gtgtacgtgc tcgaaggcgc
     1201 ggtcaccatg caggtgcaag gcagcgcacc ggtcacgttg aaacccggcg aaacttttta
     1261 cgaagcgccc ggtgatattc acgtcacgtc cgcgaacgcc agcagttcgg caccggcgaa
     1321 gttcctggtg tacatggtca aggacaaagg cgcgccggcg tcggctcccg caccggggat
     1381 cgcgcccggc ggctgagcgc gttgacagca ccggaccctc cccctatcgt ccgatccgcc
     1441 agcggcgcca accttcaaca agttggctta atagggaatc cggtgaggtt ggaaacaacc
     1501 gattccggag ctgccccgca gcggtaaccg ggaacgaccg gcaccatcag cactgggcgc
     1561 aatgcgcccg ggaagcggtg cctagtagaa aaagcgccta accacgcctc gcccggaagc
     1621 ccgaagacct gccgcggccg tcgtacgcaa gtgcgatgaa cggctctggg ctctcgcggg
     1681 aggagcggcc ggaaaatgcg ctggccgcac cgcggcctca tcgcgccttt tccgcgctcc
     1741 ttcacgtgat cccacgaaat gaaggagatt actgtgatac gagccatcaa tctcggcttc
     1801 ccgcgagtgg gggtgaagcg cgagctgaag tttgcggaag aaaactactg gaaaggcgcc
     1861 ggcacgcgcg aggcgctgct ggctaccgcg aaggacctgc ggctgcgcca ctggcggctg
     1921 cagaacgatg cgggcatcga cgacattccc tgcaacgatt tttcgctcta cgaccagatg
     1981 ctggacatga gctgcctgct cggctgcgtg ccgcggcgtt tcggctggca gagcgacgtc
     2041 gtcgacctct acacctactt cgcgatggcg cgcggctcga agaccgcagc agccatggag
     2101 atgacgaagt ggttcgacac caactaccac tacatcgtgc cggagttcga ggaaggcatg
     2161 cgcttccgca tcggcagcac caagctgttc gatgagctgg cggaggcgcg cgcggtgggc
     2221 atcgagccca cgcctgtact catcgggccg gtctcttacc tgttgctggg caaggcgaag
     2281 tatcccaact gcgatcttgc gcggatcgcc gagcggctgc tgccggtgta cgggcagatt
     2341 ctgaaatcac tggcagacca gtcggtctcg cgcgtgcaga tcgacgagcc ctgcctggtc
     2401 acggacttga gtcgcgaggc gcgcgcgctc tacccgatgg cgtaccgggc gctggcgcag
     2461 gcggcgccgc tctccatcaa tctgaccacg tacttcggtg acctcgacga caaccgccac
     2521 ctggcgttca gcctgccggt gagttccgtc catgtggacc tggcacgtgc tcccgagcag
     2581 ctcgagccct gcatcgaagc cgcgctggcc aacaacaagc ggctctcgct tggcgtggtc
     2641 gacggccgga atgtctggcg caacgatctc agccgttcgc tggccgcggt ggaacgcgcg
     2701 gcggcggtgc tgggtgagga acgcgtggcg gtcggcccgt cgtgttcgct gctgcactca
     2761 ccctgcgatc tgaaggaaga gcgcgcgatg gatcccggga tccggagctg gctggcgttc
     2821 gcaacccaga agctcgacga ggtggcagcg ctcaaacgag gcgtgacttc cggacgcagc
     2881 actatcgccc atgaactgaa gaccagcaat gacgtgcagt cggcgcgtcg cggctcgccg
     2941 cgcatccatg actcgcgcgt ggcgcagcgc gtggactcgg tgatcccacc gatgctcaat
     3001 cgcgggcgtg cattcgccga gcggcggaaa ctgcaggcct ccgagttcaa actaccgctg
     3061 tttccgacca cgaccatcgg ctcgttcccg cagaccgccg aaatccgcgc ggcgcgcgcc
     3121 gcgcacaagg cgggccggct ggagccgcag gcctacgaag cggccatgcg caaggagatc
     3181 gagggcatgg tgcgttttca ggaacgcatc gacctcgatg tactggtgca tggcgagccg
     3241 gaacgcaacg acatggtgga atacttcggc gagcagctcg agggctacgt cttcagcgaa
     3301 aacggctggg tgcagtcgta cgggtcacgc tgcgtgaaac cgcccatcat ctacggcgat
     3361 gtgtcgcgtc cgcagccgat gacggtgaaa tggtcgaagt tcgcgcagtc gctcacgccg
     3421 cgcatcatga aggggatgct cacgggtccg gtgaccatgc tgcaatggtc gttcgtgcgc
     3481 gacgatcagc cgcgcgcgca gacgtgccgg cagatcgcgc tggccattcg tgatgaagtc
     3541 ctggacctcg aggcggcggg aatccgcatc atccagatcg acgagccggc aatacgcgaa
     3601 ggactgccgc tgcgacggga acagtggcca gcctatctgc gctgggcggt ggaggcgttc
     3661 cggctgtcgg cggcgggcgt gcgggaccac acgcagatcc acacgcacat gtgctactcg
     3721 gagttcaacg acatcatgga tgcggtggcg gcgatggacg cggacgtgat ctcgatcgaa
     3781 acgtcccgct cgcacatgga gctgctcgat gcgttcgtca cgttccgtta tccgaacgag
     3841 atcggcccgg gtgtgtatga catccattcg ccgagagtgc ccagcgcgtc ggacatgggc
     3901 gcgttgctcg acaaggcgcg tggcgtgctc aaggcggaac agatctgggt gaacccggat
     3961 tgcggactca agacccgcgg ctggcccgag gtcgaacggg cgctggtgaa tatggtctcg
     4021 gtagcaaaga ccgcgcggga aacggtgtca ggcaccattt cctagcaatc ggtgccaggc
     4081 accgtttcca agaaaacggt gcctggcacc attattgggg catggacttg cccaacctgc
     4141 tgcacgagta cggctatctg ctgatcttcg tcggtgtcag gcatgcgctt cctctggggg
     4201 ctgcgcatcg cactgccgct ggcacctcgt cgacgaccgg caaatgacag attcgtccta
     4261 cactggctag cccctcctcc cgcctgcaag cgtcggcgcc tcggctagac tgccgacgat
     4321 catgttggcc cgaccctaat aatgaatctg atgatcccta ccgagccaat aggcagtatt
     4381 ccgcgcccca aggagttgat agacggcatt gccgcgcttg gcgacggctc ggatccgcgc
     4441 ctcgagccgc tttacgacgc cgccatcctg gacacggttc ggcagttcga ggccacggaa
     4501 tcgccggtga ttaccgacgg cgagcaacgc aagtaccaca acttctggac gtacagcgtc
     4561 gaaggcctgg agaccacggc gccggatggc tttcgcatac cctttgccgc cggccatgtg
     4621 cggcgcatgc ccaggcttgt gagtgggccc tttcgataca aacggtacgc ggaccgcttc
     4681 ctcgaacagg cgctgcgtca ttccacggtg ccggtcaagc aggcggtgat ctcgccgtcg
     4741 gcgctgagcc tcatgtaccc cgccgatggc atcgcaaact attcgcgcga agagttcatc
     4801 gaagacctgg tgaacgagca cgtcacggaa gtccggcgct gcctcgacat gggcgcgcac
     4861 tgcgtgcaga tcgatttcac cgagggacgc ctcgcgatca agatcgaccc caccggcgcc
     4921 ctgctcgcga gcttcatcga gctcaacaac ctggcgctct cccgcctgac gccggccgaa
     4981 cgccaacgcg tcggcgttca cacctgcccg ggtggcgatc gcgactccac gcacagcgcc
     5041 gacgtgcctt acaaagagct gctgccgagc ctgttccagc tcaacgtgac caacttctat
     5101 ctatcgctcg cgggtgagcg cgaccgcgaa ggtgcgctcg gcatcatcgc cgccaatctc
     5161 aagcccgggc atcgcgcctt catcggcgtg atcgcgccca tcgacccgcg cctcgagagc
     5221 gcggagatcg tgcgcgaccg cgtgctcgaa gcggcgcgct tcatccccgt gcaccagctc
     5281 ggcacgaccg acgattgcgg cttcgcgccg ttttgcgacg accagtcgac atcgcgcgaa
     5341 ctggcgttcg ccaagatccg cgcacgcgtc gccggcacgg cgatggcgga aaatgcattg
     5401 ggcgaacgtt agtggccacc gacgaggaag aactgaagat ccggacggcc gcgctcaaga
     5461 atatcgaggc catcctgaac gcacgccagc gcgccgaaaa cgacctcgtc gcggcgaagc
     5521 tgacgctcga aaagcgcacc gaagagttga gccagcagcg cgagtggttc gaagtgacgc
     5581 tggcgagcat cggtg
//
LOCUS       DASQJS010000077        14335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4123597, whole genome shotgun sequence.
ACCESSION   DASQJS010000077 DASQJS010000000
VERSION     DASQJS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14335
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4123597"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VEW08_03100"
     CDS             <1..460
                     /locus_tag="VEW08_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ39750.1"
                     /translation="LRMSCQTVPLAPWLRQVLLSCPPHERRVNTVQQDGIETAWMEPR
                     LMELALSNLLANALRYAKQKVNITVMRDRDLYRLVVEDDGEGIPESDRQAIFRAFTRL
                     DNSRNRDTGGFGLGLAIVARIASLHRGRVIAEASTSLGGARLALEWPDQT"
     gene            complement(451..1329)
                     /locus_tag="VEW08_03105"
     CDS             complement(451..1329)
                     /locus_tag="VEW08_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ39751.1"
                     /translation="MSASIRALQRTQQTFDSISERERLPSATLPSVTLISGRQMPVIG
                     LGTYQLKHHTVETVTQALEAGYRMFDTSGDYHTQRGIGEALRDSDVPREAIYLVTKIE
                     NTGDAFEATRANLAELKQPYADLVLIHRPPERGVGESLWQGLRRAKRLGLVTDIGVCN
                     YSIEQIEELVYRTGELPAVNQIEWSPFGHSPNMLDFCRENDIVIQAWSPLTRAQRLND
                     NKLCAMAARYGKSPAQLLLRWNLQLGVVALPKANHAQHQRENLNVFDFEISQPDMAKL
                     RTLNEHYSAMGALPYV"
     gene            1402..1740
                     /locus_tag="VEW08_03110"
     CDS             1402..1740
                     /locus_tag="VEW08_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009698244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HYJ39752.1"
                     /translation="MTTLPPCPKCKSALSYEDGVMLVCPECAHEWSKETIVAADSATA
                     VRDAHGNVLNDGDSVTVIKDLKVKGSSSVVKVGTKVKGIRLVEGDHNIDCRIEGIGEM
                     KLKSEFVKKA"
     gene            complement(1802..2434)
                     /locus_tag="VEW08_03115"
     CDS             complement(1802..2434)
                     /locus_tag="VEW08_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39753.1"
                     /translation="MKFPSFLAIAALAAAGTAAAHGGGNSSVLFKFDHGTGNQVFRSA
                     AGVPTLNTVAGVAPGGAPWGITSLDVTIKTNGDIRGRGEGVVLLGGDGLGTRAGPRQV
                     ILSLFCRNVPVPPAASAALILTPFNSEPVDLDEDGDFSVRGKLIDATGATPPLNCGDT
                     VDNRPVLLIRSVTPANPTTGTPATPGAWFAAGLLADGDRDGRGGKGDDRY"
     gene            complement(2795..4039)
                     /locus_tag="VEW08_03120"
     CDS             complement(2795..4039)
                     /locus_tag="VEW08_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ39754.1"
                     /translation="MRRLHLKLYLAIVGTLVVFLIACAMVFHHLGPPRLALSGIESAT
                     GLAATMLDARRHDLESTREILASLGYQANSDVAIFDAQGSMLLNVGRHAPTLTPAQIA
                     DSNWLITRDGPTYNHRLADGSRLIVHPRHRILAYGLHMGLLLTTIAAFLALLTYPLTR
                     GITARLARLQTGVQQFGAGNLASRVKVEGRDEVAALAKSFNESAEHIEKLVHAHQMLL
                     ANCSHELRTPLARIRLGLERVAGTDPRTNAEIARSIAELDALIGEMLLSSRLDASSGV
                     QREESVDLLALAAEEAAYFDVEASGEAVTINADAMLLRRLVRNLLENARIHAGGATGV
                     RIESDAQHARIVVEDAGGGVPLIDRERIFEPFHRASTATSSSGAGLGLAIVKKIARAH
                     EGDVSYEVLPGGGSRFTVTLPR"
     gene            complement(4040..4729)
                     /locus_tag="VEW08_03125"
     CDS             complement(4040..4729)
                     /locus_tag="VEW08_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ39755.1"
                     /translation="MTARVLLVEDDARLASMVADYLGEAGFRLGLAPNGAEAMRLIAA
                     EDFDAVILDLMLPDADGLDVCRSIRAKSDIPLLMLTARGDPLDRVVGLELGADDYLPK
                     PFEPRELLARLRAILRRRHAPGSVDVLRFGRLEIDKGAREVRLDGHRKTMTGHQFSLL
                     LSLAERAGRVLSREALMDLTRGESLEDFDRSIDVHISRLRAVIEDDPKRPRRILTLRG
                     AGYVFAKEQDR"
     gene            complement(4764..5237)
                     /locus_tag="VEW08_03130"
     CDS             complement(4764..5237)
                     /locus_tag="VEW08_03130"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HYJ39756.1"
                     /translation="MTELLSNPKRRRVGLIAIVASLVAIAGGALAYAHSGGGHHGPMS
                     GNSQRHLEHMQAMLTRIGASDAQKAQIEGILKPAFDDMKAAHESHSAAFKQFHEAITA
                     PSIDRSRLEALRAGQIKSFDEASKRLVTAISDAAEVLTPEQRAALAKQIEEHHRG"
     gene            5416..5805
                     /locus_tag="VEW08_03135"
     CDS             5416..5805
                     /locus_tag="VEW08_03135"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HYJ39757.1"
                     /translation="MKADTKYHPTPSELAILQILWSRGPSTVREIHEELAKEKDVGYT
                     SALKFLQIMTGKGLVTRTEDQRAHVYSANQPAEKTKQQFAADVLKRVFHGSASQLMQH
                     ALSGRRGSKKEIDEMRQMLDEFERKLP"
     gene            5802..7571
                     /locus_tag="VEW08_03140"
     CDS             5802..7571
                     /locus_tag="VEW08_03140"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HYJ39758.1"
                     /translation="MTPTTELMRTLAWSLLHFLWQGAAIAAVAAAFMFVFRKPATRYL
                     VGIAALALMLVSFGITFTLISGSSGAAAEVSAAGAPAAASLSSLGAHASPDFHLGEQA
                     AVSSEGGFLWIARTWLAGVFVFALRIAFGLLVIEHLRRRNLIALPDALVDRFRALQRR
                     LGIRRNVRYAECHSLSVPAVIGFFRPIVLLPMRALTGLTPEQLEAVIAHELGHIKRFD
                     VAVNFFQVIAESLFFFHPAVWWLNKRIRADREDCCDDVAIATCGGTVGYARALATMES
                     WRDVPSFAMAATGSPVTARVARLLGVSTNKGGGRTAGMVTASLVLATALIAGAVSLGV
                     AHPAMAQNTVSLEQLVVQAEVAVTPAPKVSAQPAASPEPVPAAEPAPRPASPPAPAVA
                     PKPPREPKPARPANPAAPPAPPAAPSAVSYIAEMKSSGFDNLDVDNLIAMKVHGITPE
                     YIREIRSIGFEPDAEGLIALKVQGVTPEYVRDMRALGFNPGVDDIVAMKVQNVTPEYV
                     KEMRAKGLNAGVDDLIAMKVHDVTPEYRQALERAGFKLDTSELIEAKVMDITPEFVKQ
                     AQAHGFKNLSIDQLIQLKHADVL"
     gene            7573..8433
                     /locus_tag="VEW08_03145"
     CDS             7573..8433
                     /locus_tag="VEW08_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39759.1"
                     /translation="MKTRTTAFLALVFCAFTLGFAPAASAAEEFKGNWTIRSSKQPGQ
                     VQFGLIHHRDGGRHHSESDWPVSEFKGLDLATKARHDVKFDIMRDAGRIDCEGYLNNG
                     EGAGTFGFVADAQYPKAMNALGFDGINEEMQFAMALHDVSLDFAKAVKGEKLSGLTTD
                     MLMAFRIHGVTPQFIREIRAAGLTTSDSDKLVAFRIHGVTPEMVREVRKSGLTVSEDE
                     LIAFRIHGVTPEFIAKVEKLGYQDVEPDQLVAMRIHGVTPEYIADMKARGLKNLTVDQ
                     LVNLRIHGLN"
     gene            complement(8434..11742)
                     /locus_tag="VEW08_03150"
     CDS             complement(8434..11742)
                     /locus_tag="VEW08_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HYJ39760.1"
                     /translation="MNANNWLTAFAVALLCACTPQPKVPNGTLVLDEDVTLVRQADTF
                     VDLADREITVEDDSILVAIVDENLTNVKLSLTAIGEGAPKPVEVENNLTGAGVELASL
                     EVPQGSRVRVALDAPQDATQAGKVHLRVRQYSARAVKDPKYKAQLAAFGAWTAATDAT
                     FRADAANKLGLPAIQRAIDGFEGANGDAALAAQARLIKARMFYIFSIDWREARAEAQR
                     AAAAFAKLPKPDALYEARAKYVEALALGEMSDDREAKNPTGEEAMKLAIQGLEALSAS
                     TSPLGPIERARAIGSLGHLDVKMMQADAANKHFEQARAMFESSGYVAGDREMRWNLGL
                     VLVEQGRFAEAASALEVLAPEMDRITDPELRVNAYLSVGRALTFSGQPDEGTQLLLKA
                     RPLAREYGLRAEEASALQGLGYVYQNRGDLLQATGFWEEALRLARDGKDVAAYVEALA
                     SAGQAARNDDNMKRAFELHREAVRLAPAPVPQVRTHLDLGLDYYRIQDYPGAIAEYRK
                     ALAVDLHDPMHHVYTDGKLGLAQFLFEYDKYTPADLEEASKLISEGMATSLAVNDMRS
                     IIYAVRIRAQLDARLGRNAEALEGFDKVFAMAQEYRERSASSEARSVLTYDEQLAFRG
                     YLDVIFAEAATSGPGIFRNASAREVAGLRRLERARYESFGALRVGQLDAATTAHVDKL
                     LEEMGQKSLRIAALEKTERDATQNADLHELQSAMAHLHAELDSVRTSAAAKQARAAAN
                     TPRENRNWRAIAPGAAQISYALGGKRVYAVVRGASGTRVTVLAPARRDLEAQLAAFSK
                     LDVQTRSREIEVAIEQISSVLMPAGLLPDRTTAVEIVAEGRIASIPFPALRSPTNTQL
                     RLAETHVVSMITSLFDVEESLRAQHARPYRFVALASGSGTYRAATADPAPRLQAATKE
                     IRIAADLFTAKDNSAKVKLFTGADGTGMALRDIWSSGADVVHFATHALADLRQPIASL
                     LVLPATDVNGKSTYLTAGQVQAWRGDVGLVFLSACESAIGPPQYAAGMPGLQRAFLRA
                     GARGVIATLAPIEDVFAQQFAEDFYTRFTSGQPAAQALSDTQRAWLVPKPGVSADEQL
                     RRRITALSHAYFTG"
     gene            11855..12628
                     /gene="otsB"
                     /locus_tag="VEW08_03155"
     CDS             11855..12628
                     /gene="otsB"
                     /locus_tag="VEW08_03155"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HYJ39761.1"
                     /translation="MSLRGSFLPQTNIALFLDVDGTLLEIAATPQDVRVPAALRNTLQ
                     LAARREDGALALISGRTIAELDRLFAPLVFPAAGQHGVETRDAEGNVSLPNIEAGQLD
                     PAREVIRELIRHYKGLLLEDKGSALAVHFRQAPQHERAAMEVMGELANQLRDKFVLRD
                     GKFVMELTPRGYSKRGAIESFMRQPPFAGRIPLFIGDDVTDEEGFEAVNALGGYSVRV
                     GDLAATAAKFRFSSVSAVVAWLRERNLNRPTKSQVRRKS"
     gene            complement(12643..>14335)
                     /locus_tag="VEW08_03160"
     CDS             complement(12643..>14335)
                     /locus_tag="VEW08_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYJ39762.1"
                     /translation="PATFKEAAKLLDETLQSSLRVSDKQGVLGAHRVRAQLFAEQGKP
                     AEALAEYEITFRLADEYRANSVSTEARRATHAHVQAAFRGYLDLVMSNVTARGAGNLR
                     PASKGEEAALRRLEAVREANFGATRIAALDAETTARVDELFAQMAVKSLRIAALAKRE
                     LSKTETEELSVLQGAMSDLHAELDRVRTAAATKTAVAERALPIMARELRSLTPGVVQL
                     SYALGQKYAYVWSRSESNVLVSVLGVVPQDLERGLIELGALDRQAAPDQVERSLEKMS
                     AVLMPAGLLPANSSAVEIVAEGRIAGVPFPALRSPADPKRRLVETHAITMITSLFAGD
                     EPPRLKHARPFRLVALASGTGNLRSAAVIDPAPKLQAATKEIRTVADLFVARDSTAKV
                     KLLLGAEGSAATLRGLWGSGADVVHFATHALADLRQPLASLLVLPATDAGGAPTYLTA
                     GQVEGWRGDIELVFLSACESAIGPPRFAGGMPGLQGAFLRAGARGVIATLWPIEDVLA
                     QEFSEDFYQRFTGGVSAAQALGETQRAWIAPKAGASDAEQMRRRITALAHGFYTQ"
BASE COUNT         2486 a         4718 c         4548 g         2583 t
ORIGIN      
        1 gttacgcatg agctgccaga ccgttccgct cgcgccgtgg ctgcgccagg tgctgctgtc
       61 gtgtccgccg cacgagcgac gcgtcaacac cgtgcagcag gacggcatcg aaaccgcgtg
      121 gatggaaccg cggctcatgg aactcgcgct cagcaacctg ctggccaatg ccctgcgcta
      181 cgcgaagcag aaggtgaaca tcaccgtcat gcgcgacagg gatctgtatc gcctcgtggt
      241 cgaggatgac ggcgagggca ttccggaaag cgatcgccag gcgatcttcc gcgctttcac
      301 gcgcctcgat aacagccgca accgcgacac cggtggattc ggcctggggc ttgccatcgt
      361 cgcgcgcatc gcatcgctgc atcgcgggcg ggtcatcgcc gaggccagca ccagcctcgg
      421 cggcgcgagg ttggcgctcg agtggcctga tcagacgtag ggcagcgcac ccatcgccga
      481 gtagtgctca ttgagagtgc gcagtttggc catgtccggc tggctgattt cgaaatcgaa
      541 gacgttgagg ttctcgcgct gatgttgcgc gtggttcgcc ttgggcagcg ccaccacccc
      601 gagctgcagg ttccagcgga gcaacagttg tgccgggctc ttgccgtagc gcgccgccat
      661 ggcgcagagc ttgttgtcgt tcagtcgttg cgcgcgcgtg agcgggctcc aggcctggat
      721 cacgatgtcg ttttcgcgac agaagtcgag catgttcggg ctgtgcccga acggactcca
      781 ctcgatctgg ttcaccgcgg gcaattcgcc ggtgcgatag accagctctt ctatctgctc
      841 gatggagtag ttgcacacac cgatgtcggt caccagcccc agccgcttgg cgcgccgcag
      901 accctgccac agcgattcac cgacgccgcg ttccggcggg cgatggatga gcacgaggtc
      961 ggcgtacggc tgcttgagct cggcaagatt ggcgcgcgtc gcctcgaagg cgtcacccgt
     1021 attctcgatc ttggtcacga ggtagatcgc ctcgcgcgga acatcactat cccgcagcgc
     1081 ttcaccgatg ccgcgctggg tgtggtaatc accggaggta tcgaacatgc ggtaaccggc
     1141 ctcgagagcc tgcgtcaccg tttcgacggt gtggtgtttc agttgatagg tcccgagacc
     1201 gatcaccggc atctgccggc cgctgatcag cgtcaccgac ggcagggtcg ccgacggtag
     1261 cctctcgcgc tctgatattg aatcgaaggt ttgctgcgtg cgctgcagcg cgcgtattga
     1321 ggctgacatg cggacaaggc tatggactcg cgggcagcgc ggctatcgtc gtgaatacgt
     1381 aggcatcggt tacactttag tatgactact ctgcccccct gcccaaaatg caagtccgcc
     1441 ctttcctacg aagacggcgt catgctcgta tgccccgaat gcgcacacga atggtcgaaa
     1501 gagaccatcg tcgcggccga tagcgctacc gccgtgcgcg acgcgcacgg caacgtgctc
     1561 aacgacggcg acagcgtcac cgtcatcaag gatctgaaag tgaaaggctc atcgtcggtg
     1621 gtgaaggtgg gcaccaaggt gaagggcatt cgcctcgtcg aaggcgatca caacatcgac
     1681 tgccgcatcg aaggcatcgg cgagatgaag ctgaagtcgg agttcgtgaa gaaagcctga
     1741 gggcgaacgc agaaaagcgc cggcggtcca tcgccggcgc tgccctaaca acggtcattg
     1801 atcagtaacg atcgtcgccc ttgccaccgc gtccatcgcg gtcaccgtct gcaagcagtc
     1861 ccgcagcgaa ccaggctccc ggagtcgccg gcgtacccgt ggtcggattc gcaggtgtca
     1921 cggaacggat cagcaacacc ggacggttgt cgacggtgtc gccgcaattg agcggcggcg
     1981 tcgcgcccgt ggcgtcgatg agcttgccgc gcaccgagaa gtcgccgtct tcatccagat
     2041 cgaccggctc ggagttgaac ggcgtgagga tcagcgcggc cgacgccgcc ggcggcaccg
     2101 gaacattgcg gcagaacagc gagaggatca cctggcgcgg gcccgcgcgg gtgccgagtc
     2161 catcaccgcc gagcagcacc acaccttcgc cgcgtccgcg gatgtcaccg ttggtcttga
     2221 tggtgacatc gagcgaggta atgccccagg gcgcgccgcc cggcgcaaca ccggcgaccg
     2281 tgttcagcgt gggaacgccc gcggcggaac ggaacacctg gttgcccgtg ccgtggtcaa
     2341 acttgaagag aacggaagag ttgccgccgc cgtgcgcagc cgcggtcccc gccgcggcca
     2401 gggccgcgat ggccagaaaa cttgggaact tcattggagg ctcctgcctt gtttgttgtt
     2461 accccgatga gtaacaggcg cccccgatca ggttccttta tggctcgcgt tgttgcggcg
     2521 ggccggtcat cggtgcatcc ggactcggct tgggcggcgc aggacgcact agcttgcgga
     2581 tcgccagtcg tgcggaactc gcgcgtcgac tgaagtgctc cctgagagcc tccagcgtgc
     2641 tgggtgaatt ttccatttga acctcgtcgg cctcgagcgc ctcgccaccc gcagggttgc
     2701 cgggatccac gctcgatgta accagccaac gtacctgccg gggcgcttcc tgtcggtgcg
     2761 ccaacgtacc gatggctcgc ggcgcgacgc gatatcagcg cggcagcgtc accgtgaagc
     2821 ggctgccacc accggggagc acctcatacg acacgtcgcc ctcgtgcgcg cgcgcaatct
     2881 tcttgacgat ggcgagcccg aggcccgcgc cgctcgaact tgtggcggtg ctggcgcgat
     2941 ggaacggctc gaagatccgc tcacgatcga tgagcggaac accgccgccg gcatcctcga
     3001 cgacgattcg tgcgtgctgt gcatcgcttt cgatgcgcac gccggttgcg ccaccagcat
     3061 gaatgcgcgc attttccagc aggttccgca ccaggcgccg cagcagcatc gcgtccgcgt
     3121 tgatggtcac cgcctcgccg ctggcctcga catcgaagta cgcggcttct tcggccgcaa
     3181 gcgccagcag gtcgaccgac tcctcccgtt gcacaccgct cgacgcatcg aggcggctcg
     3241 acagcagcat ttcgccaatg agcgcgtcaa gctcggcgat gctgcgtgcg atctcggcat
     3301 tcgtcctggg atccgtgccc gccacgcgct ccaggcccag gcgaatgcgc gccaatggcg
     3361 tgcgcaattc gtgggagcag ttggccaaca gcatctggtg cgcgtgtacg agcttttcga
     3421 tgtgctcggc ggattcattg aaactcttcg cgagcgccgc cacttcgtcg cgcccttcga
     3481 ccttcacgcg cgaggcaaga ttgccggcgc cgaattgctg aacgcccgtc tgcaagcgag
     3541 ccagtcgcgc cgtgattccc cgtgtgagtg gatacgtgag caacgccaga aatgcggcga
     3601 tggtagtcag cagaagtccc atgtgcaggc cgtacgccag gatgcgatgg cgtggatgca
     3661 cgatcaggcg gctgccgtcg gcgagccggt ggttgtacgt tggcccgtcg cgcgtgatca
     3721 gccaattgga atctgcaatc tgcgcgggcg tcagcgttgg cgcgtgtctg ccgacgttta
     3781 agagcatcga accttgcgcg tcgaatatcg ccacatcgga gttcgcctgg tatcccagcg
     3841 aggcgagaat ctcgcgcgtg ctctcgagat catgcctgcg agcgtcgagc atcgtggcgg
     3901 cgagacccgt ggcgctttca atgccactca atgccaggcg cggcggccca agatggtgaa
     3961 acaccattgc gcatgcgatc aggaacacca ccagcgttcc gacgatcgcc aggtagagct
     4021 tgagatggag cctgcgcaac tagcggtcct gctctttcgc gaacacgtag cccgcgccgc
     4081 gaagcgtgag aatgcggcgc ggccgcttcg gatcgtcttc gatgacggcg cgcaggcgcg
     4141 agatgtgcac atcgatcgac cggtcgaagt cctcgaggga ttcgccccgc gtgaggtcca
     4201 tcaacgcctc gcgcgacaga acgcggccgg cgcgctccgc cagcgacagc agcagcgaga
     4261 actggtggcc ggtcatggtc tttcggtgac catcgaggcg cacttcgcgt gcgcctttgt
     4321 cgatctcgag ccggccgaag cggagcacgt cgaccgagcc cggcgcatgg cggcgccgca
     4381 ggatggcgcg cagccgcgcc agcagttcgc gcggctcgaa gggcttgggt agatagtcat
     4441 cggcgccgag ctcgaggccg accacgcggt cgagcggatc gccgcgcgcg gtgagcatga
     4501 gcagggggat gtccgatttc gcgcggatac tgcggcacac gtcgaggccg tcggcgtcgg
     4561 gcagcatcag gtcgagaatg accgcgtcga aatcctccgc cgcgatgagg cgcatcgcct
     4621 cggcgccgtt cggcgcaagt cccagccgaa agcccgcctc gcccagatag tcggcgacca
     4681 tcgaggcgag gcgtgcatcg tcttcaacca gcagtactcg cgcagtcatg agctgcatat
     4741 tgcgcccgct gacttgtcgc aggctagccg cggtggtgct cctcgatctg cttcgcgagc
     4801 gccgcgcgtt gttccggagt caacacttcg gcggcatcgc tgatcgcagt gaccaggcgc
     4861 ttcgaggctt catcgaacga cttgatctgg ccggcgcgca gcgcttcgag ccgcgagcga
     4921 tcgatgctcg gcgcggtgat cgcttcgtgg aactgcttga acgctgcgga atgcgattcg
     4981 tgcgcggcct tcatgtcgtc gaacgccggc ttgagaatgc cttcgatctg cgccttctgc
     5041 gcgtccgacg cgccgatcct ggtcagcatc gcctgcatgt gctcgaggtg ccgctgcgag
     5101 ttgccggaca tcgggccgtg atggccgcca cccgagtgcg cataagccag cgcgcctccg
     5161 gcgatcgcga ccagcgacgc gacgatggca atgaggccaa cgcggcgacg ctttggattg
     5221 gaaagtagct cagtcatggg tcgtactccg tgtgtgtcag ggcggaagca gccttgctca
     5281 gcgtcgtttc cccctgatcg cgcgtgtgta aagttttgta aagcccgggg cccgcaggac
     5341 gaaatccgtt ttttttggtt catctacgaa gaccttcgtt gacagtacga aatacttcgt
     5401 agtagctttg cccccatgaa agcagacacg aaataccacc ccacgccctc ggagctcgcg
     5461 atcctccaga tcctctggtc gcgcggccct tccacggttc gcgaaatcca tgaagagctc
     5521 gcgaaggaaa aggacgtcgg atacaccagc gcgctcaagt tcctgcagat catgaccggc
     5581 aaggggctcg tcacgcgcac tgaagaccag cgcgcccacg tttattcagc gaaccagcct
     5641 gccgaaaaaa ccaagcagca gttcgcggcg gatgtgctga agcgcgtatt ccacggatcc
     5701 gccagccagc tcatgcaaca cgccctgtcc ggccggcgcg gctcgaaaaa ggaaatcgac
     5761 gagatgcgcc agatgctcga cgaattcgaa aggaaactcc catgacaccg actactgaac
     5821 tcatgcgcac gctcgcgtgg tcattgctgc acttcctgtg gcagggcgcc gccatcgctg
     5881 ccgtcgccgc ggcattcatg ttcgtgttcc gcaagccggc gacgcgttac ctggtcggca
     5941 tcgccgcact cgcgctgatg ctggtgtcat tcggaatcac gttcacgctc atcagcggct
     6001 cttccggagc cgccgctgaa gtttccgctg caggggcgcc agccgccgcc tcgctctcct
     6061 cccttggagc acatgcaagc ccggactttc acctgggcga gcaagcggct gtttcttccg
     6121 aaggcggatt tctctggatc gcgcgcacct ggctggccgg cgtcttcgtg ttcgcactgc
     6181 gaattgcatt cggcctcctg gtgatcgaac acctgcgccg gcgcaatctc atcgcgctgc
     6241 ccgatgcgtt ggtagatcgc ttccgggcgc tacagcgccg tctcggcatt cgccgcaacg
     6301 ttcgctacgc ggaatgccac tccctcagcg ttcccgcggt catcggtttc ttccggccca
     6361 tcgtgctgct gccgatgcgc gcgctgaccg gcctcacccc cgaacaactc gaggccgtca
     6421 tcgcgcatga actcgggcac atcaagcgct tcgatgtcgc cgtgaacttc ttccaggtca
     6481 tcgccgaatc gctgttcttc tttcatcccg ccgtgtggtg gctcaacaag cgcatccgcg
     6541 cggatcgtga ggattgttgt gacgacgtcg ccatcgccac gtgcggcggc accgtcggtt
     6601 atgcccgtgc gctcgccacc atggagagct ggcgggacgt gccgagtttc gccatggccg
     6661 ccacgggcag cccggtgacc gcgcgcgtcg cgcgcctgct cggcgtcagc actaacaaag
     6721 gcggcggacg cacggccggc atggtgacgg cttcgctggt gcttgccacc gcgctcatcg
     6781 ccggcgcggt ttcgctcggc gtcgcacacc ccgcgatggc ccagaacaca gtgtcgctgg
     6841 aacagctggt cgtccaggcc gaagttgcgg tcacgcctgc gccaaaggtt tcggcgcaac
     6901 cagccgcatc tccagagcca gtcccggcag cggaaccggc accgcgtccg gcaagcccgc
     6961 cggcacccgc ggtcgctcca aaaccgccgc gtgagccaaa gcccgcgcgc ccggcaaatc
     7021 ccgcggcacc accggcaccg cccgctgcgc caagcgcagt ctcgtacatc gcggagatga
     7081 agtcctcggg cttcgacaac ctcgatgtcg acaacctgat tgcgatgaag gtccacggaa
     7141 tcacacccga atacattcgc gagattcgga gcatcggctt cgagccggat gcggaaggcc
     7201 tcatcgcgct gaaagtccag ggagtgacac cggagtacgt tcgcgacatg cgcgcgctgg
     7261 gtttcaatcc cggcgtggac gatatcgtgg cgatgaaggt ccagaacgtg acccccgagt
     7321 acgtgaagga gatgcgcgcg aaaggcctga acgccggtgt cgacgacctc atcgccatga
     7381 aggttcacga cgtcacgccc gagtatcgcc aggcgctcga acgcgcgggc ttcaagctcg
     7441 acacctccga actcatcgag gcgaaggtca tggacatcac gccggaattc gtcaagcagg
     7501 cgcaggcgca cggcttcaag aatctcagca tcgaccagtt gatccagctc aaacacgcgg
     7561 atgtgctctg acatgaaaac tcgaacgacg gcattcctgg ccctggtgtt ctgcgcattc
     7621 acgctcgggt tcgcgcccgc ggcgagcgcc gccgaggagt tcaagggcaa ctggacgatc
     7681 aggtcgtcga agcagcccgg gcaggttcaa ttcggcttga tccatcatcg tgacggcggc
     7741 agacatcaca gcgaatcgga ttggccggtc agcgagttca aaggtctcga tctcgcgacg
     7801 aaggccagac acgatgtgaa gttcgacatc atgcgtgacg ccggccgcat cgactgcgag
     7861 ggttacctca acaacggcga aggcgccggc accttcggct ttgtggccga tgcgcaatat
     7921 cccaaggcca tgaatgcgct gggcttcgac ggcatcaacg aggaaatgca gttcgcgatg
     7981 gcgctgcacg acgtatcgct cgacttcgcc aaagcggtca agggcgaaaa gttatccggc
     8041 ctcaccaccg atatgctcat ggcgtttcgc attcatggcg tcacaccgca gttcatccgc
     8101 gagattcgtg ccgctggcct gacaaccagc gacagcgaca agctggtggc attccgcatc
     8161 catggcgtca cgccggagat ggtgcgcgag gttcgcaagt cgggcctgac ggtgagcgag
     8221 gacgagctca tcgcgttccg cattcacggc gtgacgccgg aattcatcgc caaggtcgag
     8281 aagctcggct accaggatgt cgagccggat caattggtgg cgatgcgcat ccatggcgtg
     8341 acgccggaat acatcgccga catgaaggca cgcgggctga agaacctgac cgtcgaccag
     8401 ctggtcaacc tgcgcatcca cggtctcaat tagctagccc gtgaagtacg cgtgtgacag
     8461 cgccgtgatg cggcggcgca gctgctcgtc cgcactcacg ccgggcttcg gaaccagcca
     8521 tgcgcgctgc gtgtcgctca gggcctgcgc ggccggctgc ccgctcgtga aacgcgtgta
     8581 gaaatcctcg gcgaactgct gggcaaacac atcttcgatg ggcgccagcg tggcgatgac
     8641 gccgcgcgcg cctgcgcgca ggaacgcgcg ctgcagtccg ggcatgccgg cggcatactg
     8701 cggcgggccg atggcactct cgcaggcgct gaggaacacc agtccgacgt cgccgcgcca
     8761 ggcctgcacc tggcccgcgg tgagataggt ggacttgccg ttcacgtcgg tggcaggcag
     8821 cacgagcagg gaagcaatgg gctggcgaag atcggcgagt gcgtgcgtgg cgaagtgcac
     8881 cacgtccgcg ccgctcgacc agatgtcgcg cagcgccatg cccgtgccgt ccgcgcccgt
     8941 gaatagtttg accttcgccg aattgtcttt ggcggtgaac agatccgccg cgatgcggat
     9001 ctctttcgtt gccgcctgca gcctcggcgc cggatcggcg gtcgccgcgc gatacgttcc
     9061 gctgccgctc gcgagcgcca cgaaccgata ggggcgtgca tgctgggcgc gcaacgattc
     9121 ttcgacgtcg aacagcgaag tgatcatcga aacgacgtgg gtttccgcca accgcagttg
     9181 cgtgttggtt ggtgaacgta atgcagggaa agggatgctg gcgatgcgac cttcggcgac
     9241 gatttcgacc gcagtggtcc tgtccggcag cagtccggcg ggcatcagta cggatgagat
     9301 ctgctcgatc gccacttcta tctcgcgcga tctggtctgc acgtccagct tcgagaacgc
     9361 ggccagttgc gcctccaggt ctctgcgcgc aggcgccagc accgtcacgc gcgtgccgga
     9421 tgcaccgcgc accaccgcgt agacgcgctt accaccgagc gcataagaaa tttgcgccgc
     9481 gccgggcgcg atggcgcgcc agttacgatt ctcgcgcggc gtattcgcgg ccgcacgcgc
     9541 ctgcttcgcc gccgccgagg tgcgcacgct gtcgagctca gcgtgcagat gcgccatggc
     9601 gctctgcagt tcgtgcaggt ccgcattctg cgttgcgtcg cgctccgtct tttccaatgc
     9661 cgcgatccgc aggctctttt gccccatctc ctccagcaac ttgtcgacgt gagcggtggt
     9721 cgcggcgtcg agctgcccga cgcgcaatgc gccaaaactt tcgtagcgtg cccgctccag
     9781 ccgccgcaga cccgccactt cgcgtgccga cgcgttccgg aaaatgcccg ggccactggt
     9841 cgccgcttcc gcgaagatca catcgagata gccacgaaag gcgagctgct cgtcgtacgt
     9901 cagcaccgag cgcgcttcgc tgctcgcgct gcgctcgcga tattcctgag ccatcgcgaa
     9961 caccttgtcg aagccctcga gagcttctgc gttccgtccg agccgcgcgt ccagctgggc
    10021 tcgaatgcga accgcgtaga tgatgctgcg catgtcgttc acggccaggc tggtggccat
    10081 cccctcggat atgagcttcg acgcttcctc gagatccgcg ggtgtgtact tgtcgtactc
    10141 gaagaggaat tgcgccaggc ccaactttcc gtcggtgtag acgtggtgca tggggtcgtg
    10201 caggtccact gccagcgcct tgcgatactc ggcgatggcg ccggggtagt cctggatgcg
    10261 gtagtagtcg agtcccagat ccagatgcgt gcgcacctga gggacgggcg ccggcgccag
    10321 tcgtaccgct tcccggtgca actcgaaggc gcgcttcatg ttgtcatcgt tgcgcgccgc
    10381 ctggccagcc gatgccaggg cctcgacgta ggccgccacg tcctttccat cgcgcgcgag
    10441 cctgagcgcc tcttcccaga acccggttgc ctgcagcagg tccccgcgat tctgataaac
    10501 atagcccaga ccctgaagtg cgctcgcctc ctcggcgcgc aacccgtact cgcgggccag
    10561 cggccgggcc ttcagcagca gttgcgtgcc ttcgtcgggt tgcccggaga atgtgagtgc
    10621 ccgccccacc gacagatatg cgttaacgcg cagctcgggg tccgtgatcc tgtccatctc
    10681 gggcgccagc acctccagcg cactcgccgc ttcggcgaac cggccttgtt ctaccagtac
    10741 cagccccagg ttccagcgca tctcgcggtc gcccgcgacg tagccgctcg attcgaacat
    10801 ggcgcgcgcc tgctcgaaat gtttgttagc cgcgtctgcc tgcatcatct tgacgtcgag
    10861 atgacccagc gagccgatgg cgcgcgcacg ctcgatgggt cctaacggcg aagtgcttgc
    10921 gctcaaggcc tcgagaccct ggatcgccag cttcatggcc tcttctcccg tggggttctt
    10981 cgcctcgcga tcgtcggaca tctcgcccag cgcgagcgcc tccacgtatt tcgcgcgcgc
    11041 ttcgtagagc gcatcgggtt tgggcagctt tgcgaatgcc gccgcggcac gctgagcctc
    11101 cgcacgcgct tcacgccagt cgatgctgaa gatgtaaaac atccgcgcct tgatgagacg
    11161 cgcttgtgcc gcgagcgctg catccccatt cgcaccttcg aatccatcaa ttgcgcgctg
    11221 tatggcgggc aaacccagct tgttggcagc gtccgccctg aaggtcgcgt ccgtggcagc
    11281 cgtccaggcg ccgaatgccg cgagttgcgc cttgtacttc ggatccttga cggcacgcgc
    11341 cgagtattgc cgcactcgca ggtgaacctt gccggcctgg gttgcgtctt ggggcgcatc
    11401 gagcgccacg cgaacacgcg atccctgggg gacctccagc gacgcaagct cgacgccggc
    11461 gccggtcagg ttgttttcca cttcgacggg ttttggcgcc ccttcaccaa tcgctgtgag
    11521 gctgagcttt acgttcgtga ggttctcgtc gacgatcgcg accagaatgg agtcgtcctc
    11581 gacggtgatt tcgcggtccg ccaggtcgac aaacgtgtcg gcctggcgca cgagtgtcac
    11641 gtcctcgtcg agcaccaacg tgccattggg caccttcggt tgcggcgtgc aagcgcacag
    11701 cagagccacg gcgaacgccg tcagccagtt atttgcattc atccctgaag cctccccgaa
    11761 gacggggaaa gcttaatgga gatcggtgga aaaggggacg ctggcaggcg ctatctagcg
    11821 gcgcgccggc cgcgaaattc ggtatgtttg ccacatgagt cttcgcggat ccttcctgcc
    11881 gcaaaccaat atcgcgctgt tcctagacgt ggatggcacc ttgttggaga ttgcagccac
    11941 gcctcaggat gtgcgcgtcc ccgcggcgct tcgcaatacg ctgcagcttg cggccaggcg
    12001 cgaggatggc gcgctggctt tgatcagcgg ccgcacgatc gccgagctcg accgcctgtt
    12061 cgcgcccctg gtgttccccg ccgccggcca gcacggcgtc gaaacccgcg acgccgaggg
    12121 caacgtgtcc ctgcccaaca tcgaggccgg gcaactggat ccggcgcgcg aggtcatccg
    12181 cgagctcatc cggcattaca aaggcctgct gttggaagac aagggatcgg cgctggcggt
    12241 acatttccgg caggcgccgc agcacgaacg ggccgccatg gaagtgatgg gagagctcgc
    12301 gaaccagctg cgagacaagt tcgtgctgcg cgatggcaag ttcgtcatgg aactcacgcc
    12361 gcgcggctat tcgaagcgcg gtgcaatcga atcgttcatg cgccagccgc cgtttgcagg
    12421 ccgcatcccc ttgttcatcg gcgacgacgt caccgacgaa gagggattcg aggcagtcaa
    12481 cgcgcttggt ggctattcgg tacgcgtcgg cgacctggcg gccacggcgg cgaagtttcg
    12541 cttcagcagt gtttcggccg tggtcgcgtg gctgcgcgag cgcaacctga accgtcccac
    12601 aaagtcacag gtgcgccgca agagttagcg gcgcccatgg cttcattggg tatagaaacc
    12661 gtgcgccagc gccgtgatgc gccggcgcat ttgttctgcg tcgctggcgc cggctttcgg
    12721 cgcaatccag gcgcgctgcg tttcgccaag agcctgcgcc gccgagacgc cgccagtaaa
    12781 tcgctggtag aaatcctcgg agaattcctg cgccagcacg tcttcgatgg gccacagcgt
    12841 cgcgatgaca ccgcgcgcgc cggcgcgcag gaaagccccc tgcaggccgg gcatgccgcc
    12901 ggcaaaacgc ggtggaccga ttgcgctctc gcaggcgctc aggaacacga gctcgatgtc
    12961 tccgcgccat ccctccacct gccccgcggt caggtaggta ggtgcgcctc cggcgtcggt
    13021 cgccggcaac acgagcagcg atgccagcgg ttggcgcagg tccgccagcg cgtgtgtggc
    13081 gaagtgcacc acgtcggcgc cgctacccca gaggccgcgc aatgtcgcgg cgctgccttc
    13141 cgcgccgagc agtagtttga ctttcgcggt ggagtcgcgg gcgacgaaca gatccgcgac
    13201 agtgcggatc tctttcgtcg ccgcctgaag tttcggtgcc gggtcgatca cggcggccga
    13261 ccgcaaattg ccggtgccgc tggcgagcgc caccagccga aacggacgcg cgtgcttcag
    13321 gcgcggcggc tcatcaccgg caaacagcga agtgatcatc gtgatcgcgt gcgtttcgac
    13381 cagccgccgc ttcgggtccg ccggcgaccg tagtgccgga aatggcacac cggcgatgcg
    13441 gccctcggcc acgatttcca ccgcggatga attcgccggc agaaggcccg cgggcatcaa
    13501 cacggccgac atcttttcga gtgaccgctc gacctgatcg ggtgccgcct gccggtcgag
    13561 cgcgccgagc tcgatcaatc cgcgctcgag gtcctgtggc accaccccga gtacggacac
    13621 cagaacattc gattcgctgc gtgaccacac gtatgcgtac ttctgaccga gcgcgtagga
    13681 aagctgcacg acgccgggcg tcaggctgcg caattcgcgc gccatgatcg gcagggccct
    13741 ttccgcaacc gccgtcttgg tcgccgccgc ggtacgcaca cgatcgagct cggcgtgcag
    13801 atccgacatt gccccctgca acaccgacaa ctcttccgtc tccgtcttcg acagttcgcg
    13861 cttcgccagc gccgcgatcc tcaggctctt gacggccatc tgggcgaaca attcatccac
    13921 gcgtgcggtc gtttccgcat cgagcgcggc aatgcgcgtt gcaccgaagt tcgcctcgcg
    13981 cacggcctcg agtctgcgca acgcagcttc ttcacccttg gaagcgggcc tcaggtttcc
    14041 cgcgccccga gccgtcacgt tgctcatgac caggtccagg tagccgcgaa atgccgcctg
    14101 cacatgtgca tgcgtcgcgc ggcgagcttc cgtgctgacg ctgttggcgc ggtattcatc
    14161 cgcaagccgg aaggtgattt cgtactccgc cagtgcctct gcaggtttgc cctgttccgc
    14221 gaagagctgc gccctgacgc ggtgcgcgcc gagcacaccc tgcttgtcgc tgacccgcag
    14281 cgagctttgc aaagtctcgt cgagcagttt cgccgcctcc ttgaatgtgg caggc
//
LOCUS       DASQJS010000078         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_983692, whole genome shotgun sequence.
ACCESSION   DASQJS010000078 DASQJS010000000
VERSION     DASQJS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983692"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VEW08_03165"
     CDS             complement(<1..439)
                     /locus_tag="VEW08_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HYJ39763.1"
                     /translation="MSFKSKFITAAAGVGFATLPFMQATAGDVTVGGGMRTSFSSTEV
                     DFDDGEGGTGSDSSSDFALNSARLYISGKATDNIGFMFNTEYNSNDEEIRVIDAAAQF
                     SFSGGTHNIWVGRFLPPSDRANLYGPYYASNWAVYQDGVQDGYP"
     gene            683..>2076
                     /locus_tag="VEW08_03170"
     CDS             683..>2076
                     /locus_tag="VEW08_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39764.1"
                     /translation="MSTKPRLQKIIRERRQYNQWVASQTLEDYALRFTAQSARKSTAR
                     VGNTALGPIAFLACEAIGGTLTLLYGFPNVAWAIGAFAVLMFLIGLPIAFYAARYGVD
                     IDLLTRGAGFGYMGSTVTSLIYASFTFLLFAIEASIMSVALNLVFDIPLWIAHIFSSL
                     VVIPIALYGISLISKMQIITQPIWLILQFFPIAIIAWKHPAEIAAWTQYGGTETSTEG
                     AGGASFNFALFGLAASVLLSLLPQIGEQVDYLRFLPNKTRANRVGWWTAVIGTGPGWV
                     LLGGFKLLIGSFLAVWLVRQGVHPTQADEPTIMYHHAFREVFQSPTFALVLTGIFVIV
                     CQLKINVTNAYAGSIAWSNFFSRLTHAHPGRVVWLVFNVVLALLLMEIGIFRAIESIL
                     VIYANFAAGWIGALTADLVINKPLGFSPKYIEFKRAHLYDINPVGVGALAISVVLSSA
                     AFMGAFGLYAQILSP"
BASE COUNT          361 a          645 c          627 g          443 t
ORIGIN      
        1 acggatagcc gtcctgcacg ccgtcctggt acacggccca gttgcttgcg tagtaaggac
       61 cataaagatt ggcgcgatcg ctgggcggca ggaagcggcc cacccagatg ttgtgcgtgc
      121 caccgctgaa ggaaaattgc gccgccgcgt cgatgacacg gatctcttca tcgttggagt
      181 tgtactccgt gttgaacatg aagccgatgt tgtcagtggc cttgccgctg atgtacaggc
      241 gcgcgctgtt caacgcgaag tcgctggacg agtcgctgcc cgtgccgcct tcaccgtcgt
      301 cgaaatcgac ctcggtgctc gagaagctgg tacgcatacc gccgccgacc gtaacgtcgc
      361 ccgccgtggc ttgcatgaat ggcagcgtcg cgaaaccaac gcccgctgcc gccgtgatga
      421 attttgattt gaatgacatg ttgttccctg ttggctatcc ctgcgatcga cggtacgaaa
      481 cgggttccat cggaggtagg cgtcatttga cgtaggcgtt cgcgcagttg ggcgaactcg
      541 gtgcggattc acgaacccct gcactcgaaa gtcgcagttg tacgaatcgt gcgcagccgg
      601 tcgcgttacc ggcacatcgg ccgccccctc cgcatggcac aacgcttgct atctagttat
      661 ggggaacagg ggatacatcg gcgtgagcac gaagccgaga cttcagaaaa tcattcgcga
      721 acgccgccag tacaaccagt gggtggcgag ccagactctc gaggactatg cgctgcgttt
      781 cacggcgcag agtgcgcgaa agtcaactgc gcgcgtcgga aacacggcgc tcggacccat
      841 cgcgtttctc gcctgcgaag cgatcggcgg cacgctcacg ctgctgtacg gatttccgaa
      901 cgtcgcctgg gccatcggcg cgttcgcggt gctcatgttc ctcatcggac tgcccatcgc
      961 gttctacgcc gccaggtacg gcgtcgacat cgacctgctc acgcgcggcg ccggtttcgg
     1021 ctacatgggc tcgaccgtca cttcgctgat ttacgcctcg ttcacgttcc tgctgttcgc
     1081 catcgaggcc agcatcatgt cggtggcgct gaacctggtg ttcgacatcc ccctgtggat
     1141 cgcgcacatc ttcagctcgc tggtggtgat ccccattgcc ctctacggca taagtctcat
     1201 cagcaagatg cagatcatca cccagccgat ctggctgatc ctgcagttct ttcccatcgc
     1261 catcatcgcg tggaagcatc cggccgagat cgccgcgtgg acccagtacg gtggcactga
     1321 aacctcgact gaaggagccg ggggcgccag cttcaatttt gcgttgttcg ggctcgcggc
     1381 gtcggtgctt ctgtcgcttc tgccgcagat cggcgaacag gtggactacc tgcggttcct
     1441 gccgaacaag acgcgggcca atcgcgtcgg gtggtggacg gcggtcatcg gcaccggacc
     1501 aggatgggtg ctgcttggcg gattcaaact actgatcggc tcgttcctgg ctgtgtggct
     1561 ggtgcgccag ggcgtgcatc ccacgcaggc ggacgagccg acgatcatgt atcaccacgc
     1621 gttccgcgaa gtattccagt cgccgacgtt cgccctggtg ctgaccggca tctttgtgat
     1681 cgtctgccag ctcaagatca acgtgacgaa tgcctacgcg ggctcgatcg cctggtccaa
     1741 cttcttctcg cgcctgaccc atgcgcaccc gggacgcgtc gtatggctgg tgttcaacgt
     1801 ggtgctggcg ttgttgctca tggagatcgg catctttcgc gccatcgaga gcattctcgt
     1861 gatctatgcc aacttcgccg ccggttggat aggcgcgctc acggccgacc tcgtcatcaa
     1921 caagccgctg ggtttcagcc cgaagtacat cgaattcaag cgtgcgcatc tctacgacat
     1981 caacccggtg ggcgtgggag cactggccat ttccgtagtg ctgtcgagcg cggcgttcat
     2041 gggcgccttc gggctgtacg cgcagatcct ctcgcc
//
LOCUS       DASQJS010000079         5483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2243813, whole genome shotgun sequence.
ACCESSION   DASQJS010000079 DASQJS010000000
VERSION     DASQJS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2243813"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VEW08_03175"
     CDS             complement(<1..237)
                     /locus_tag="VEW08_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004694369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39765.1"
                     /translation="MHAPIQSVTSLKPVPGGRPRAGASKLTHLARLEAESIHIFREVA
                     AEFAKPLLMYSIGKDSSVLLHLARKAFFPGRIPFP"
     gene            complement(329..2629)
                     /locus_tag="VEW08_03180"
     CDS             complement(329..2629)
                     /locus_tag="VEW08_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HYJ39766.1"
                     /translation="MTTPTTPPPSDFHINEIHYDNVGTDAGEAIEIEGPAGADINGFS
                     IVLFNGDALSGAAYNTQPLSGFLPTSCGTRGVFAVTYPPPGIQNGSPDGIALVNSSGV
                     VLEFLSYEGVFTATTAPAAGLTSVDILASQNSAALGSSLQRSSAGIWAPGASSFGACN
                     PEAPTPVGNSLQFVGRVPSDPALPVGYEDQIFATLRSPGNVTIPTTITWASETPAIAS
                     ITQAGVIRALSEGMATFRATAAEGTTATYSLPTSVAVASGVAYPGNTEFGEPTDADAS
                     DDFIVRHEQFTASYNPNRGSPNWVSYDLDAAHFGTEDRCDCFTMDADLPASFPQITTD
                     DYTGAGAFHGYPIDRGHMVRSFDRTTGSLDNARTYLFSNVVPQAAEMNQGPWAMLENA
                     LGNFARNDNREVYIITGPAGNRGTLRDLGRVVIPDSTWKVAVIMAHDAGLASIADYRD
                     LQVIAVNMPNDDGIRNVPWQTYLTTVDAIEALTGYDLLALLPDDVEAAVESNTQPPIA
                     AIAGPPGAIAEGDTASFDAAGSLDPNGTVASYSWDFGDGSIGSGVSVTHTYAQDGAFA
                     VRVTVTDNDGLTDSATFMVNVTNVAPVVGAVPDGELNVGATYTATGSFTDPGADAWTA
                     TVNWGDGSALEQVALSGRSFSLTHIYMAAGTYTVSIDIVDDDAAGSDAHTVTVAQPAP
                     GLAEALPLIDQLIASRKMPRAVGLVLKAEIIAAQVLIGRGNAPAATGLLRAIVAEIDF
                     LVRLRVVNAGDVAPLRSLLVGVIGTL"
     gene            complement(2626..3423)
                     /locus_tag="VEW08_03185"
     CDS             complement(2626..3423)
                     /locus_tag="VEW08_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39767.1"
                     /translation="MRMNNLGRWLTAAAALTLSSFATAQVRISELHYDNDGADVGEAV
                     EVSAPAGTNLTGWRIVLYNGSGGASYGTLPLSGVMPATCGARGVLVVAGPATGIQNGS
                     PDGLALVNAADAVVEFLSYEGVFAATSVPATGITSVDIGVTEAGNEPAGMSLARDAAG
                     TWSGPATATFGACNDDGETPPTPEVATVNVSPTNATITVGANRSFTGTALDINSQPIN
                     GTPFTWTSTIPWLRQSARPVSPRRWPRAIRASSPPPKTVSPDRPRCT"
     gene            complement(3578..4291)
                     /locus_tag="VEW08_03190"
     CDS             complement(3578..4291)
                     /locus_tag="VEW08_03190"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HYJ39768.1"
                     /translation="MKSLIVTLIAALLPLSAIAADAPAPKTTKPMKIGIIGTGDIGGA
                     LARHWSAAGHQLLVSSRHPEQLQTLANELGPNVKVGTPREAAAFGDVVMLAVPYGATP
                     QVGRDYAAELKGKVVLDAGNPYPSRDGDMAVRDRQRGTGVASAEYLPGTRLVRAFNAI
                     NAGPLAREAFRKPERIGIPLAADDAKAMEIAGQLVSDAGFDPVPVGKLSRARDFDYGT
                     PVYVRGMTAAELRKVLKLQ"
     gene            complement(4385..5362)
                     /locus_tag="VEW08_03195"
     CDS             complement(4385..5362)
                     /locus_tag="VEW08_03195"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HYJ39769.1"
                     /translation="MKLQQLRYLAAVVQNGLNVTTAAQALHTSQPGVSKQIRLLEEEL
                     GLTLFVRDGRALKRLTPAGEEVAARALRMLREAQALKSLAKDLKQSDRGSLSIGTTHT
                     QARYVLPRVIRDFRQKYPDVQLHLHQGTSDQIAEMASLDRIDFAINTGSQDKFPDFVL
                     LPIYSWHRQIIVPKDHPLAQIAKPTLQQLAEYPLVTYVFSFTGPSSLHQQFARAGLEP
                     NVSLTARDADVIKTYVRLGLGVGVVASMAIEPEDEKDLAVVEAGHLFPSHVTWIGFRS
                     GALLRGFTYDFIQLLAPHLTRERIERILSAPTPAAREKLITAIELPRYD"
     gene            complement(5393..>5483)
                     /gene="rpsJ"
                     /locus_tag="VEW08_03200"
                     /gene_synonym="nusE"
     CDS             complement(5393..>5483)
                     /gene="rpsJ"
                     /locus_tag="VEW08_03200"
                     /gene_synonym="nusE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929568.1"
                     /note="NusE; involved in assembly of the 30S subunit; in
                     the ribosome, this protein is involved in the binding of
                     tRNA; in Escherichia coli this protein was also found to
                     be involved in transcription antitermination; NusB/S10
                     heterodimers bind boxA sequences in the leader RNA of rrn
                     operons which is required for antitermination; binding of
                     NusB/S10 to boxA nucleates assembly of the antitermination
                     complex; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HYJ39770.1"
                     /translation="DIINPTDKTVDALMKLELPAGVDVQLKLN"
BASE COUNT          937 a         1704 c         1826 g         1016 t
ORIGIN      
        1 cgggaaagga atacggcccg ggaaaaaggc cttgcgggcg aggtgcagca ggaccgagga
       61 gtccttgccg atggaataca tgagcagcgg cttggcgaat tcggccgcca cttcgcggaa
      121 gatgtggatg ctttcggcct cgaggcgagc caggtgggtc agtttggacg cgcccgcgcg
      181 aggacgcccc ccgggaacgg gtttcaggga cgtaactgac tgaatcgggg cgtgcatcta
      241 gagccttccg ggaaaagtgg gcgggactgt atatgtcgcc accccccgcc cggaaagcat
      301 ttctggttat gcaggaataa cagggcggtt acagggtgcc gatgaccccc accagcaggc
      361 tgcgcagcgg ggccacatcc ccggcgttga ccactcgcag acgcaccaga aagtcgattt
      421 ccgccacaat ggccctcaaa agccccgtcg cggccggcgc atttccacgg ccgatgagca
      481 cctgcgcggc gatgatttcc gccttcagca ccaaccccac ggcgcggggc atcttgcggc
      541 tggcgatgag ctggtcgatg agcggcagcg cttcggcgag gcccggcgcg ggctgggcga
      601 cggtcacggt atgcgcgtcc gatccggccg cgtcgtcgtc cacgatgtcg atgctgaccg
      661 tgtaggttcc cgcggccatg tagatatgcg tgagtgagaa gctgcggccg ctcaacgcca
      721 cctgctcgag ggcactgcca tcgccccagt tcaccgtcgc agtccaggcg tcggcgccgg
      781 gatcggtaaa cgagccggtg gcggtgtaag tggcaccgac gttcagctca ccatccggca
      841 ctgctcctac gactggcgcg acgttggtca cgttcaccat gaacgtcgcc gaatcggtca
      901 gaccatcatt gtcggtgact gtcacgcgaa ccgcgaacgc cccgtcctgc gcatacgtgt
      961 gtgtcaccga cacgccgctg ccgatcgatc cgtcaccgaa gtcccaggag taactggcca
     1021 cggtaccgtt cgggtcgagc gaaccggccg cgtcgaatga agccgtgtct ccctcggcga
     1081 tggcgccagg cgggcctgca atcgcggcga tcggcggctg ggtgttggat tcgaccgcgg
     1141 cctcgacatc atcgggcagc aacgcgagca ggtcgtaacc ggtcagcgcc tcgatcgcat
     1201 ccactgtagt cagatacgtc tgccatggaa cgttgcgtat tccatcatcg ttcggcatgt
     1261 tgacggcgat cacctgcaga tcgcggtagt cggcgatgct tgccagcccc gcgtcgtgcg
     1321 ccatgatcac cgcgaccttc caggtggagt cggggatcac caccctgccg agatctctga
     1381 gcgtgccgcg gttaccggcc ggacccgtga tgatgtacac ctcgcggttg tcgttgcgcg
     1441 cgaaattccc gagcgcgttc tcgagcatcg cccacggccc ctggttcatc tcggccgcct
     1501 gcggcacgac gttgctgaat agataggtgc gtgcgttgtc gagactgccc gtggtgcgat
     1561 cgaatgagcg caccatgtgg ccgcggtcga tgggatagcc gtggaaagcg ccggcgccgg
     1621 tgtagtcgtc ggtcgttatc tgcgggaacg aggccgggag gtctgcatcc atggtgaagc
     1681 agtcgcagcg atcctcggtg ccgaagtgcg ccgcatcgag gtcgtagctc acccagttgg
     1741 gtgatccgcg attcgggttg tacgacgccg tgaactgttc atggcgcacg atgaagtcgt
     1801 cgctggcatc ggcgtcggtg ggctccccaa attccgtgtt tcccggatac gccacgccac
     1861 tcgccacggc cacgctcgtc ggcagcgagt aggtcgccgt ggtgccctcg gcggcggtcg
     1921 cgcggaacgt cgccattccc tcgctcaacg cgcgaattac gcctgcctgg gtaatcgagg
     1981 cgatcgcggg ggtttccgac gcccacgtga tagtggtcgg aatcgtcacg ttgcccgggc
     2041 tgcgcagtgt tgcgaagatc tggtcttcgt agcccaccgg caatgcggga tcgctcggta
     2101 cacgacccac gaattgcagt gagttgccga ccggggtcgg agcctccggg ttgcaggcac
     2161 caaagctcga cgcgccgggc gcccagatgc cagcagaact tctctgcagc gatgagccga
     2221 gagccgcact gttctgagac gcgaggatgt cgacggacgt caggccggcg gcgggagccg
     2281 tggtggccgt gaatacgccc tcgtacgaga gaaactccag cacgacccct gacgaattca
     2341 ccagcgcaat gccgtcgggc gaaccgtttt gaatgccggg tggtggatac gtcacagcga
     2401 acactccgcg cgtgccgcag ctcgtcggga ggaatccact caagggctgt gtgttgtacg
     2461 cggcgccgct gagtgcatcg ccgttgaaca agactatgct gaatccgttg atgtccgcgc
     2521 ccgcgggccc ttcgatctcg atggcttcac ccgcatccgt gccaacgttg tcgtagtgga
     2581 tctcgttgat gtggaagtcg gacggcggcg gtgtcgtcgg agtcgtcacg tgcagcgcgg
     2641 ccgatccggc gacaccgttt tcggcggtgg cgacgatgct cgtatcgccc tcggccagcg
     2701 ccgtggcgac accggtcgcg ctgactgacg caaccagggg attgtgctcg tccaggtaaa
     2761 cggtgtgccg ttgatcggct ggctattgat gtccagagcg gtgccggtga acgaccgatt
     2821 cgcgcccaca gtgatcgtcg cgttcgttgg cgaaacgttg accgtcgcca cttcgggagt
     2881 cggtggcgtc tcgccatcgt cgttgcaggc gccgaaggtc gcggtcgccg gcccgctcca
     2941 ggtaccagca gcatcgcgcg ccagcgacat tccagccggt tcgttgcccg cttcggtgac
     3001 gccgatatcg acggacgtga ttcccgtcgc tggaacactc gtcgcggcga acacgccttc
     3061 gtacgacagg aattcgacaa ccgcgtcggc agcgtttacc agcgctagtc catccggtga
     3121 gccgttctga attccggtgg ccggacccgc gaccaccagc acgccccgcg cgccgcaggt
     3181 ggcaggcatc acgcccgaaa gcggcagcgt gccgtacgaa gctccaccgc tgccgttata
     3241 gagaacgatg cgccagccgg tcaggttcgt ccccgcggga gctgacactt ccacggcttc
     3301 gccaacatcg gcgccgtcgt tgtcgtagtg gagttcgctg atgcgcacct gggcggtggc
     3361 gaaagagctc aatgtcagag cggctgcagc agtcagccag cggcctagat tattcatgcg
     3421 catgtttcca tccctgtgcg tttttgttcg atcacgcaag tttcagaact tgcgattcat
     3481 ggcgtccgag gatacgccaa tgcgaacact cgctcgtggc agggagatga cgcgtggttt
     3541 tcgttctgtg gaaaaagtcc gcgagccgca gaaaccacta ttgcagtttc aataccttgc
     3601 gcaattccgc ggcggtcatg ccgcgaacgt acaccggtgt gccgtagtcg aagtcgcgcg
     3661 cgcgcgaaag cttgcccacg ggaacggggt cgaagccggc atccgacacg agctggcccg
     3721 caatttccat cgccttcgca tcgtcggccg caagcggaat gccgattcgt tccggcttgc
     3781 ggaatgcctc gcgtgccagc gggcccgcgt tgatggcgtt gaacgcccgc accaggcgcg
     3841 tgcccggcaa gtactcggcc gaggccacgc ccgtgccgcg ctggcggtcg cgcacggcca
     3901 tgtcgccgtc gcgtgaggga taaggattac cggcgtcgag cacgaccttg cccttgagct
     3961 cggcggcgta gtcgcggccc acctgcggcg tcgcgccgta tggcaccgcg agcatcacca
     4021 cgtcgccaaa tgcggctgct tcgcgcggcg tgcccacctt cacgttcggc cccagctcat
     4081 tggcgagcgt ctgcagttgc tcgggatggc gcgaagaaac gagcagttgg tgacctgcgg
     4141 cggaccaatg acgcgcgagc gcgccaccga tgtcacccgt gccgatgatg ccgatcttca
     4201 tgggcttggt ggttttcggg gctggggcgt ccgcggcaat tgccgaaagc ggcagcaagg
     4261 cggcgataag ggtgacgatc agggatttca tcggtgacct ccagttcaca gcttcgatga
     4321 tcaggctgtc atcgggttca atcgcttcag cgcgtgaatc gctccgttca ggtgcgaccc
     4381 attttcagtc gtagcgcggc agctcgatcg ccgtgatgag tttctcgcgc gccgcgggcg
     4441 tgggagccga gagaatgcgc tcgatgcgct cgcgcgtgag atgtggcgcg agcagctgga
     4501 tgaaatcgta ggtgaagccg cgcagcagcg cgcctgagcg aaacccgatc caggtaacgt
     4561 ggctcgggaa caagtgcccc gcttcgacga cggccaggtc cttttcgtct tctggctcga
     4621 tggccatgct ggcgacgacg cccacaccca accccaggcg cacgtaagtc ttgatgacgt
     4681 cggcatcgcg cgctgtcagc gacacgttcg gttcgagccc ggcgcgcgcg aactgctgat
     4741 gcagcgacga cgggcccgtg aagctgaaca cgtaggtgac cagcggatat tcggccagct
     4801 gctgcagcgt gggcttggcg atctgcgcca gcggatggtc cttcggcacg atgatctgcc
     4861 gatgccacga atagataggc agcagcacga agtcggggaa tttgtcctgg ctgccggtgt
     4921 tgatcgcgaa gtcgatgcga tcgagcgacg ccatctcggc gatctggtcc gaggtgccct
     4981 ggtgcaggtg cagctggacg tcgggatact tctggcggaa atcgcggatc acgcgcggca
     5041 ggacgtaacg cgcctgcgtg tgcgtggtgc cgatggacag cgagccgcgg tcggattgct
     5101 tgaggtcttt ggccaggctc ttgagcgcct gcgcctcgcg cagcatgcgc agcgcgcgcg
     5161 ccgccacttc ttcgccggcg ggcgtcaggc gcttgagggc gcggccgtca cgcacgaaca
     5221 gcgtgaggcc gagctcctct tcaagcagac ggatctgctt gctgacgcca ggctgtgaag
     5281 tgtgcagcgc ctgggcggca gtcgtgacgt tgaggccgtt ctgcacgacg gccgccaggt
     5341 aacgaagttg ctgcagtttc aaatcgagac tccgcagcga attgctttgt tcttagttga
     5401 gcttgagctg cacgtcgacg ccggcgggca gttcgagctt catgagcgca tccaccgtct
     5461 tatcggtcgg gttgatgatg tcc
//
LOCUS       DASQJS010000080         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2244097, whole genome shotgun sequence.
ACCESSION   DASQJS010000080 DASQJS010000000
VERSION     DASQJS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2244097"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2214
                     /locus_tag="VEW08_03205"
     CDS             <1..>2214
                     /locus_tag="VEW08_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYJ39771.1"
                     /translation="GSANIDTNSRLCMASAVAGHKRAFGEDVVPVSYDDLDQADLIVL
                     VGSNTAWCHPVILQRILAARDKRPNLKIVALDPRRTATTQISDMHLPLAAGTDVHLFN
                     GLLVWLAEHGHLDQSFIDAHTVGWGEALQAARAGGGDLQATATACGLDVTELRSFYEL
                     FAQTPAVITAFSQGVNQSSSGTDKVNAIINCHLATGRIGKTGAGPFSVTGQPNAMGGR
                     EVGGFVNTLAAHVDLHDEPGRAAVKSFWHSPAIASKQGPKAVEMFDRIHEGKIKAVWI
                     MATNPVVSLPDADKVKEALRNCEFVAVSDVMGNTDTAALAHVLLPALAWGEKDGTVTN
                     SDRTISRQRPFLPPPGEARADWRMVCDVARAMGFEGFNFISPHEIFREHAQLTAHHND
                     GRRMLNLGAWANITAAEYKNWEPAAWPMAEARESASGPMFADGKFMHPDGKARFLPLT
                     PRLPENAPTQEFPLVFNTGRVRDHWHTMTRTGKSARLSAHVAEPYAEINTADALRFKV
                     RAGELATLRSKWGSMVVRVRTGADIPSGMVFSPIHWNRAFASDARVGALTNPVVDPIS
                     GEPELKHTPVAIEHFDASWYAVMLTRSPLPPPATQWWTKVQGEKFVRYELAGNHSRDW
                     SIEARRLLGAPAGDSIADVDWIEYRDPARRVYRAAWFVGDRLEGCIYFDRLPALPERA
                     WLARMFTMAKCDAPVRASLMAGRELEGVDQGALVCSCFGVGTNPIAACARELGAAA"
BASE COUNT          372 a          728 c          744 g          370 t
ORIGIN      
        1 gggcagcgcc aatatcgaca ccaactcgag gctgtgcatg gcgtcggcgg tggccgggca
       61 caagcgcgcg ttcggcgaag acgtcgtgcc agtgagttat gacgatctcg accaggccga
      121 tctcatcgtg ttggtcggtt cgaacacggc ctggtgccac cccgtcatcc tgcagcgcat
      181 cctggcagcc cgcgacaaac gcccgaacct gaagatcgtc gcgctcgatc cgcggcgcac
      241 cgcgacgacc cagatctccg atatgcacct gccgctcgcg gccggcaccg acgtgcacct
      301 gttcaacggg ttgttagtgt ggctcgcgga acacggccac ctggatcagt ccttcatcga
      361 tgcccatacc gtgggctggg gcgaggcgct gcaggcggcg cgagcgggcg gcggcgatct
      421 gcaggccacg gccacggcct gcggccttga cgtcaccgag ctgcgcagct tttacgagtt
      481 gtttgcgcaa actcccgcgg tgatcaccgc attctcgcag ggcgtgaacc agagctcgtc
      541 gggcaccgac aaggtcaacg ccatcatcaa ctgccatctc gccacgggcc gcatcggcaa
      601 aaccggcgcg ggaccgtttt cagtgaccgg ccaaccgaat gccatgggtg gccgcgaagt
      661 gggtggcttc gtcaacacgc tggcggcgca cgtcgacctg cacgacgagc cgggccgtgc
      721 ggcggtgaag agtttctggc acagccccgc catcgccagc aaacaaggcc cgaaagcggt
      781 cgagatgttc gatcgcattc acgaaggcaa gatcaaggcg gtgtggatca tggccaccaa
      841 cccggtggtc agtctccccg atgccgacaa ggtgaaagag gcgctgcgca actgcgagtt
      901 cgtcgccgtg tccgatgtca tgggcaacac cgacaccgcg gcgctggcgc acgtgctctt
      961 gccggcgctg gcctggggtg agaaagatgg cacggttacc aactccgatc gcaccatctc
     1021 gcggcagcgc ccgttcctgc cgccgcccgg cgaagcgcgt gcggactggc gcatggtctg
     1081 cgacgtggcg cgcgccatgg gcttcgaggg attcaatttc atttcgccgc acgagatctt
     1141 ccgcgaacat gcacagctca cggcgcatca caacgatggc cggcgcatgc tgaacctcgg
     1201 cgcgtgggcg aacatcacgg ccgccgaata caagaactgg gagcccgcgg cctggcccat
     1261 ggccgaggcg cgcgagtcgg cgagcggccc catgttcgcc gatggcaaat tcatgcatcc
     1321 cgacggcaag gcgcgcttcc tgccgctgac gccgcgcttg cccgaaaacg cccccacgca
     1381 ggaattcccg ctggtgttca acaccggccg cgtgcgcgat cactggcaca ccatgacgcg
     1441 caccgggaag tccgcgcggc tgtccgcgca tgtggccgag ccttatgccg aaatcaacac
     1501 cgccgatgcg ctgcgcttca aggtgcgtgc cggtgagctg gcgacgctgc gctcgaaatg
     1561 gggctcgatg gtagtccggg tgcgcacggg ggcggacatt ccttcgggca tggtgttttc
     1621 gcctatccac tggaatcgcg ccttcgcgtc cgatgcgcgc gtaggcgcgc tcacgaatcc
     1681 cgtggtcgat ccgatatccg gcgagccgga gctcaagcac acgccggtgg ccatcgagca
     1741 cttcgacgcg tcgtggtacg cggtgatgct cacgcgcagt ccgctgccgc cgccggccac
     1801 gcagtggtgg acgaaggttc agggcgagaa gttcgtgcgt tacgagctcg ccggcaatca
     1861 ttcgcgcgac tggtcgatag aggcgcggcg gttgctgggc gcgcctgccg gcgattcaat
     1921 cgccgacgtc gactggatcg agtatcgcga tccggcgagg cgcgtgtatc gcgcggcctg
     1981 gttcgttggt gaccggctcg aaggatgtat ttatttcgac cgcctgccgg cgctgccgga
     2041 gcgcgcctgg ctggctcgca tgttcacgat ggcgaaatgc gatgcgccgg tgcgcgccag
     2101 tctcatggcc ggacgcgagc tcgagggtgt cgaccagggg gcgctggtgt gctcgtgctt
     2161 cggcgtgggg accaatccca ttgccgcctg tgctcgcgaa ctcggcgccg cggc
//
LOCUS       DASQJS010000081         3598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2244108, whole genome shotgun sequence.
ACCESSION   DASQJS010000081 DASQJS010000000
VERSION     DASQJS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2244108"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VEW08_03210"
     CDS             <1..629
                     /locus_tag="VEW08_03210"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HYJ39772.1"
                     /translation="TKWTLHGREFANCNCSYGCPCQFNALPTHGNCWAVVGIEIDKGN
                     HGKVPLDGLRLAGIFRYPGAIHMGNGEAVPIVDKRANEAQRNALLRIMSGQDTRPGAT
                     MFQVFFSMLTKVHDPVFADIDFDVDIDKRRAHLRVPGYIEQRGEPIVNPVSGAEHRIR
                     IEPVGGFEFKVAEIGRGWTKASGPISYELADSYGQFAELHLCQDGIIN"
     gene            605..1411
                     /locus_tag="VEW08_03215"
     CDS             605..1411
                     /locus_tag="VEW08_03215"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HYJ39773.1"
                     /translation="MSGRHHQLIGAAPLEFALRRDRWVIVGALLSLTILALGYTYWLG
                     TGFDMSGMMSPRFIPWTAAHFAFMFAMWLIMMIGMMTPSVAPMVLLYVAVGRSSVGAG
                     QGFAPASAFVAGYLLSWGAFSAAATLLQWLLESRALITPMMAGTSRPLAGAILIAAGV
                     YQWLPIKQSCLSQCRAPLSFVQRHGGFQASARGSLRLGVLHGWYCIGCCWVVMALLFA
                     FGVMNFYWIAGLMLFVLIEKVLPYAGIVSRVAGLVAVCAGIAMMVALPIS"
     gene            complement(1461..2120)
                     /gene="gstA"
                     /locus_tag="VEW08_03220"
     CDS             complement(1461..2120)
                     /gene="gstA"
                     /locus_tag="VEW08_03220"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HYJ39774.1"
                     /translation="MPTSRNKTHEMEFLMQLYFSPGACSLASHITAREAGLSLDLKRA
                     DTKTKKLEDGSDYFAINSKGAVPALKLDNGQVLTEGVAIMQYLADQKPESNLAPKNGT
                     LERYRVQEWLNYVTSEVHKTFSPLWNPANEQAVKDYALANLKKKFDWLNGQLAGKKYL
                     TGDNFTVADAYLFTVVNWSNFLAIDLSAWPALKEFQARVAARPKVQEAMEAEGLIKKA
                     A"
     gene            2236..2598
                     /locus_tag="VEW08_03225"
     CDS             2236..2598
                     /locus_tag="VEW08_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ39775.1"
                     /translation="MPKAQCAANVFSSACPSRRIMAVLAEKWTLLIVTQLANGPMRTA
                     EIRRGVDGVSEKMLIQTLRKLEGFGLISRKSYPEVPPRVEYRLTALGRSLSRLAGLFS
                     RWVEKNAANLLKAEQEAA"
     gene            2766..3317
                     /locus_tag="VEW08_03230"
     CDS             2766..3317
                     /locus_tag="VEW08_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39776.1"
                     /translation="MNTPKTKTLSRRASVLSCVILMLVANVARPGAAVPGEELAVLRA
                     IAGIVNRDATRPYERLYFQSEFEGASFVATSLENPDRNQFCGLSRPEALTMVSELELA
                     TARRVQLDKETAKSAGLKIGRWKDPRFPYIVLSRVYFGPGRQSAWVGVELNGKAGAVM
                     RLDKVGGEWKKTSRCGGWIKYED"
     gene            complement(3371..>3598)
                     /locus_tag="VEW08_03235"
     CDS             complement(3371..>3598)
                     /locus_tag="VEW08_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39777.1"
                     /translation="IDSATALHLTIAIFMAANYASCPRHATAGAAVCDLRRRRTPSAR
                     KIPRRAAVEASSRGFTLVLRRMAIGPSTGQL"
BASE COUNT          642 a         1051 c         1168 g          737 t
ORIGIN      
        1 acacgaagtg gaccctgcac gggcgagagt ttgccaactg caactgctcc tacggttgcc
       61 cctgccaatt caacgcgctg ccgacgcacg gaaattgctg ggcggtggta ggcatcgaaa
      121 tcgacaaggg caatcacggc aaggtgccgc tcgatgggct gcggctcgcc ggcattttca
      181 gatacccggg cgccatccac atgggcaatg gcgaagcagt gccgatcgtc gacaagcgcg
      241 caaatgaagc gcagcgcaat gcgctgttgc gcatcatgag tggccaggac acgcgcccgg
      301 gcgcgacgat gttccaggtc ttcttctcca tgctgaccaa ggttcacgat ccggtcttcg
      361 ccgacatcga tttcgacgtg gacatcgaca agcgacgcgc acacctgcgc gtgccgggat
      421 acatcgagca gcgcggcgag cccatcgtca atccggtgtc gggggcggag catcgcatcc
      481 gcatcgagcc cgtcggcggg ttcgaattca aggtcgccga gatcggccga gggtggacga
      541 aggcgtcggg gccgatcagc tacgagctgg cggattccta tggtcagttc gccgagctgc
      601 acctttgtca ggacggcatc atcaactgat cggcgcggcg cccctcgagt tcgcactgcg
      661 acgtgaccgc tgggtcatcg tcggcgcgtt gctgtcgctg accattctcg cgctcggcta
      721 cacgtactgg ctcggcacag gattcgacat gtcggggatg atgtcgccgc gcttcatccc
      781 gtggaccgcg gctcacttcg cgttcatgtt cgcgatgtgg ctgatcatga tgatcggcat
      841 gatgacgccg tcggtggcgc cgatggtgtt gctgtatgtg gcggtcgggc ggagcagcgt
      901 cggggcaggg caggggttcg caccggccag cgcattcgtc gcgggctatc tactgtcgtg
      961 gggcgcattc tcggcagccg cgacgttgct gcaatggctg ctggagtcgc gcgcactcat
     1021 cacaccgatg atggcgggca cgagccggcc gctcgccggc gcgatattga tcgccgccgg
     1081 tgtctaccag tggctgccca tcaagcagtc gtgcctttca caatgccgcg cgccgctgtc
     1141 gttcgtgcag cgtcacggcg gctttcaggc gagcgcgcgc ggttcgctgc gactcggcgt
     1201 gttgcatggc tggtactgca tcggctgctg ctgggtcgtc atggcgctgc tgttcgcctt
     1261 cggcgtcatg aacttctact ggatcgccgg gctgatgttg ttcgtgctca tcgagaaggt
     1321 gttgccgtac gcggggatag tgtcgcgagt ggcgggcttg gtcgcggtct gcgccggtat
     1381 cgccatgatg gtggcgctgc ccatttccta gctaaacgaa tggggacaga cttaagtctg
     1441 tccccacacg tccttgtccc ttacgcggct ttcttgatca gcccctcggc ctccatggct
     1501 tcctgtactt tcggacgcgc ggcgacgcgc gcctggaatt ccttcaacgc cggccaggcg
     1561 ctgaggtcga tggcgaggaa gttggaccag ttcaccacgg tgaacagata cgcatcggcg
     1621 acggtgaagt tgtctccggt caggtacttc ttgccggcga gctggccgtt gagccagtcg
     1681 aacttcttct tgaggttcgc gagcgcgtag tccttcacgg cctgctcgtt cgccgggttc
     1741 cacagcggcg aaaaggtctt gtgtacttcg ctggtgacgt agttgagcca ctcctgaacg
     1801 cgataacgct cgagcgtacc gttcttcggc gcgaggttgc tctcgggctt ctggtcggcg
     1861 aggtactgca tgatggccac gccttcggtg agcacctggc cattgtcgag cttgagcgcg
     1921 ggcacggcgc ccttggaatt gattgcgaag tagtcggagc catcctccag cttcttcgtc
     1981 ttggtgtcgg cgcgcttgag gtcgagcgac aaaccggctt cgcgcgcggt gatgtgggag
     2041 gcgagcgagc aggcgccggg ggagaaatac aactgcatga gaaactccat ttcgtgggtc
     2101 ttgtttcggg aggtgggcat tctgtagacg cacccaccag cgcacaagta cttacctggc
     2161 ggtaagtacc cagccagacg cgtatgtggg tacttacctt ttggtaagta cccctgctgt
     2221 ggtacggtgc cgcccatgcc aaaagctcaa tgtgctgcaa acgtattcag ttcggcgtgc
     2281 ccatcgcgcc gcatcatggc cgtgctcgcc gagaaatgga cgctgctcat cgtcacgcag
     2341 ctcgcgaacg ggcccatgcg caccgcggaa attcgccgcg gcgtggatgg cgtgtcagag
     2401 aagatgctca tccagacgct gcgcaagctc gaaggcttcg ggctcatcag ccgcaagagt
     2461 tatcccgaag tgccgccgcg cgtcgagtat cggctaacgg cactgggacg ctcgctgtcg
     2521 cggctggcgg gtttgttcag ccggtgggtc gaaaaaaatg ccgcgaacct gctgaaggcg
     2581 gagcaagagg ccgcatgaaa tccatgcttg cgtgagaaaa attcatcgct gaccaggtgt
     2641 cactacgcac cgcgttagag cgacgtcaat aaaccgcgcg cgctaaatgg gcgagaccga
     2701 cgcagttcgc aatgcgctga ttcggcagtg gcatggtgcg gatggcttgc gtagtttccg
     2761 ccgccatgaa cacaccgaaa acaaaaacac tttctcgaag ggcgtccgtc ctctcgtgcg
     2821 tgatcctgat gctggtggcc aatgtcgcga gacctggcgc cgccgttcct ggcgaagagc
     2881 ttgcggtgct gcgggcgatt gccgggatcg tcaatcgcga cgccacccgt ccctatgaac
     2941 gcctttattt ccagtcggag ttcgaaggcg catcgttcgt cgccacttcc ctcgagaatc
     3001 cggatcgcaa tcaattttgc gggctttcac gcccggaagc gctgacaatg gtttccgagc
     3061 tggagttagc gaccgcccgg cgcgtgcaac tcgacaagga aactgcgaag tcggcgggct
     3121 tgaagatcgg ccgctggaaa gacccccgct tcccctacat agtcctttcg cgcgtgtact
     3181 tcggtccggg cagacaatcc gcgtgggtcg gagttgaatt gaacgggaag gcgggcgccg
     3241 tcatgcggct cgacaaagtc ggcggcgaat ggaaaaagac ttcgcgatgt ggtggctgga
     3301 tcaagtatga ggactgacgt cagggcgact gaaggttttc caggatcgat ccagtcttgc
     3361 gtgcaagcga tcacaattgt ccggtagatg ggccgatcgc catgcggcga agcactaggg
     3421 taaaacctcg tgagctcgcc tccacggcgg cgcggcgcgg aatcttccgc gcacttgggg
     3481 tgcgacgccg acgcaggtcg caaactgctg caccggcagt ggcatggcgc ggacagcttg
     3541 cgtaatttgc cgccatgaaa attgctatcg ttaaatgcag cgcggttgcg ctgtcgat
//
LOCUS       DASQJS010000082         5856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4124688, whole genome shotgun sequence.
ACCESSION   DASQJS010000082 DASQJS010000000
VERSION     DASQJS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4124688"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /gene="aceE"
                     /locus_tag="VEW08_03240"
     CDS             <1..774
                     /gene="aceE"
                     /locus_tag="VEW08_03240"
                     /inference="COORDINATES: protein motif:HMM:NF036501.4"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HYJ39778.1"
                     /translation="RTTLAGEGLQHQDGHSLLHAATVPNCVAYDPTYAYELAVIIQDG
                     LKRMFANDESVFYYIAVMNENYVQPALPKGVEEGILKGLYLLQTGGAGKVRVSLLGSG
                     TILREVIAAAEILSRDFGIPSDIYSATSFSELRREALEIERWNRLHPGETARTPYVTQ
                     ALSKATGPFIAATDYMRAWPDMIRQWVPGRFTTLGTDGFGRSDGRTALRKHFEVDAKN
                     IVYASLHALAEEGALDKGTLKAALNKLGLDANKLDPQKA"
     gene            771..2405
                     /locus_tag="VEW08_03245"
     CDS             771..2405
                     /locus_tag="VEW08_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HYJ39779.1"
                     /translation="MSTVDVSVPDMGNFESVTVIDVLVKPGDTIDVDTPLVTLETDKA
                     TMDVPSTAKGVVEKVHVTKGGSVSTGALIVTVKSAAGAAAPTPATKAAASAPAPAAAQ
                     AAPPAAAAKPASIDVNVPDMGSFDSVAVIDVLVKPGDAVDIDTPLVTLETDKATMDVP
                     STAKGVVEKVHVAKGGKVSPGAKVVTVKGVAASATPAPAPAQPAATTSNKPVSQTRPA
                     APLAVQQMMAPAARGGLPPIDEASFGAAHASPSVRKFARELGVNLGSVRGSGEKGRIL
                     LDDVKAFVKMVMAGGGPVAAPALPKAHNYDPATFGAVEVKPLNRVQKISGPRLQAAWI
                     NIPHVTQFEESDITDLEALRGSLKEKAAAAGVKVTPLAFIIEAAVRAMREFPVLNSQL
                     DESGQNLIFKKFFNVGFAADTPNGLLVPVIKAADTLDVFAIARALGDLSGKARDGKLP
                     GADMQGAGFTISSLGGVGGTMFTPIINSPEVAILGVSKSAMKPVWNGKEFAPRLMLPL
                     SLSYDHRVIDGAMGARIARFLADTLAKPKDLVGAMS"
     gene            2402..2617
                     /locus_tag="VEW08_03250"
                     /pseudo
     CDS             2402..2617
                     /locus_tag="VEW08_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
     gene            2795..4213
                     /gene="lpdA"
                     /locus_tag="VEW08_03255"
     CDS             2795..4213
                     /gene="lpdA"
                     /locus_tag="VEW08_03255"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HYJ39780.1"
                     /translation="MPHDRETQLLVLGAGPGGYTAAFRAADLGLKVTLVERWPMLGGV
                     CLNVGCIPSKALLHAAKVIDEVGEMSHHGVEYGAPKIDLAKLLGWKNSVVKKLVGGLS
                     VLAKQRKVEVVTGVGKFASPHVMEITGADGKSQKIRFEQCIIAAGSEAIKLPFIPDDP
                     RVIDSTGALELGGVPKRLLVIGGGIIGLEMATVYAALGAKLSVVELTGQLMPGADVDL
                     VRPLEKRLKARYEQILLNTKVTGCTAKAEGLVVEFEDANGKRTDTFDRVLVAVGRTAN
                     GKRIGLENADIDVSDRGVIPVDKQMRTNLPHVFAIGDLVPGPMLAHKAMHEAKVAAEV
                     AAGHKSFFDARVIPSVAYTDPEVAWVGLTETEAKKNGTQYKKGQFPWAASGRSLALGR
                     DEGFSKLLFDPVSHRVLGGGMVGPNAGELVAEVALAIEMGADAADIGLTIHPHPTLSE
                     TVALSAEAFEGTITDLYMPRKA"
     gene            complement(4307..5074)
                     /locus_tag="VEW08_03260"
     CDS             complement(4307..5074)
                     /locus_tag="VEW08_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39781.1"
                     /translation="MGISETIIAAIIGALATMLTAIVQLVRNRAPSDGRPKPKKNRIR
                     SVLATAALMIGCIVGGYAWSSLRAVSAKEELAATMEVEFTKQFAALAARQNPESAAGS
                     TNGGVDASGAAIPARNGEAGSAESLAHLPPCKVSAHPDEVGPVTCSDRVAVPIALCAS
                     IPSAALATNVRVQARVPKSESPWLERDAGAPTLGNLHIAAAPVEYPVSADQRSVCLDV
                     ANFSVDDTLAVRVIVEYAFAGPSPGELTAAAPSAANL"
     gene            complement(5212..5640)
                     /locus_tag="VEW08_03265"
     CDS             complement(5212..5640)
                     /locus_tag="VEW08_03265"
                     /inference="COORDINATES: protein motif:HMM:NF020471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HYJ39782.1"
                     /translation="MIFAGQSRDQLRETYRAAWRRFKQQHTLSPLDRQVVAVISEHPE
                     YHVIVESAAADLANYSPRSGQLNPWLHMGLHLAVREQVATNRPAGIADLHAKLSRQLG
                     GAHEAEHRMLEVLAEQLWEAQRGGKPPDENAYLERLRGLT"
     gene            complement(5637..>5856)
                     /locus_tag="VEW08_03270"
     CDS             complement(5637..>5856)
                     /locus_tag="VEW08_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYJ39783.1"
                     /translation="TRIAVGETPRAVEDALVKHTPGEFALDAHHWLILHGRYVCKALR
                     PECERCLVNTLCEYPLRHKIIAAAKKAP"
BASE COUNT         1057 a         1849 c         1970 g          980 t
ORIGIN      
        1 cgcaccacgc tcgcgggcga aggactgcag caccaggacg gacacagcct gctgcatgcg
       61 gcgacggtgc cgaattgcgt ggcctacgac ccgacgtatg cctacgaact cgccgtgatc
      121 atccaggatg gtctcaagcg catgttcgcc aacgacgaga gcgtgttcta ttacatcgcg
      181 gtgatgaacg agaactacgt tcagccggcg ttacccaagg gcgtcgagga gggaatcctc
      241 aagggcctgt acctgctgca gactggtggc gccggaaagg tgcgcgtctc gctgctgggc
      301 agcggcacca tcctgcgcga ggttatagcc gcggcagaaa tattgtcccg cgacttcggc
      361 attccctcgg atatctattc ggcgacgagt ttctccgagc tgcgccgcga ggcgctcgag
      421 atcgagcgat ggaaccgcct gcacccgggt gagaccgcgc gtacgccgta cgtcacgcag
      481 gccttgtcca aggccaccgg tccgttcatc gcggccaccg actacatgcg cgcctggccg
      541 gacatgatcc ggcaatgggt acccggacga ttcaccacgt tgggaaccga cggattcggc
      601 aggtccgacg gccgcaccgc gctgcgcaag cacttcgaag tggacgcgaa gaacatcgtc
      661 tacgcgtcgt tgcacgcgct cgccgaagaa ggtgccctcg acaagggcac gctcaaggcc
      721 gcgctcaaca agcttggcct cgacgccaac aagctggatc cgcagaaagc atgagcacgg
      781 ttgacgtaag cgtccccgac atggggaatt tcgagtcggt cacggtcatc gacgtgttgg
      841 tgaagcccgg cgacaccatc gacgtggaca cgccgctggt cacgctggaa accgacaagg
      901 ccaccatgga tgtgccctcg accgccaagg gcgtcgtcga gaaggtgcac gtcaccaaag
      961 ggggttcggt atccacgggt gcgctgatcg tcaccgtgaa gtcggccgca ggtgcggccg
     1021 ctccgacgcc ggcaaccaaa gctgcagctt ctgctccggc tccagccgct gcccaggccg
     1081 cgccgcccgc ggccgccgcc aagccggcca gcatcgacgt caacgtgccc gacatgggca
     1141 gtttcgattc ggtggccgtc atcgacgtgc tggtgaagcc aggcgacgcc gtggatatcg
     1201 acacaccgct ggtcacgctg gaaaccgaca aggccaccat ggatgtgccg tcgaccgcca
     1261 agggcgtcgt cgagaaggtg cacgttgcaa agggtggcaa ggtatcgccc ggcgcgaagg
     1321 tcgtgaccgt caagggtgtg gccgcgtcgg cgacgcctgc gccagctccc gcgcagccgg
     1381 cagccaccac gtctaacaag cccgtgtcgc agacgcgtcc ggccgcgccc cttgcagtgc
     1441 agcagatgat ggcgccggct gcccgtggtg gcctgccgcc catcgacgaa gccagtttcg
     1501 gcgctgctca tgcctcgccc tcggttcgca agttcgcgcg cgaacttggc gtcaatctcg
     1561 ggagtgtgcg tggcagcggc gagaagggcc gcatcctgct cgatgacgtc aaagcctttg
     1621 taaagatggt gatggcgggt ggaggtccgg tcgcagctcc cgctctgccc aaggcccaca
     1681 actacgaccc ggccacattc ggcgcggtcg aagtcaaacc gctcaaccgc gtgcagaaaa
     1741 tctccggtcc gcgtctgcag gccgcatgga tcaacattcc gcatgtcacg cagttcgaag
     1801 aatccgacat cacggatctc gaggcgctgc gtggttcgct caaggaaaag gccgcggccg
     1861 cgggcgtgaa agtcacgccg ctggcgttca tcatcgaggc ggcggtgcgc gcgatgcgcg
     1921 aattccccgt gctcaacagc cagcttgatg agtcgggcca gaatctcatc ttcaagaagt
     1981 tcttcaacgt ggggttcgcg gcggatacgc ccaacggcct gctcgtgccg gtgatcaagg
     2041 ccgccgacac gctggacgtg ttcgccatcg cacgagcgct gggcgatctg agtggcaaag
     2101 cccgcgacgg caaactaccc ggcgccgaca tgcagggggc ggggttcacc atctcgagcc
     2161 tgggcggcgt gggcggcacc atgttcacgc ccatcatcaa ttcgcccgaa gtcgcgattc
     2221 tcggcgtttc gaagtcggcg atgaagcccg tctggaacgg caaggagttc gcgccgcggc
     2281 tcatgttgcc gctgtcgctc tcctatgatc accgcgtcat cgatggcgcg atgggcgccc
     2341 gcatcgccag gttcctggcc gacacgctcg ccaagccgaa ggatctcgtc ggggcgatgt
     2401 cgtgagcaag atcgacctga ctgttcccga cctcggaagt ttcgacgagg tcgccatcat
     2461 cgacgtgctg gtcaaggtgg gcgacaagat cgaagtggaa acaccactgg tcacgctcga
     2521 aaccgacaag gcgacgatgg acgtgccgtc caccgcggcc ggaaccgtca ctgaagtgct
     2581 ggtacagaag ggcggcaaga tcggcaaggg tggcgtgatc gcgcgcatcg aaggtgttgc
     2641 cgctgcggcg gcgcccgcga aagcgccggt tggcgatgcg ccggccgcgg ctgctcccgc
     2701 cgcgaaggcg gcagcaccgg cttccaccgc ggcaccggcg ccgcgcgagg ctcccgcccc
     2761 cgcaccgaaa ccgtcgaagc cgctcaagcc cgacgtgccg cacgatcgcg aaacccagtt
     2821 gttggttctg ggcgctggtc ccggcggcta caccgccgcg ttccgcgctg ccgatctggg
     2881 cctcaaagtg acgctggtcg aacgctggcc catgttgggc ggcgtttgtc tgaacgtcgg
     2941 ctgcattccg tccaaggcgc tgctgcacgc cgccaaggtc atcgacgaag tcggtgagat
     3001 gagtcaccac ggcgtcgaat acggtgcgcc gaaaatcgac ctggccaaac tgctcggctg
     3061 gaagaacagc gtggtcaaga agctggtcgg cggcctgtcg gtgctggcga agcagcgcaa
     3121 ggtcgaggtg gtcacgggtg tgggcaagtt cgcgagcccg cacgtcatgg agatcaccgg
     3181 cgcggatggc aagagtcaga agatccgctt cgagcaatgc atcattgccg ccggctccga
     3241 ggccatcaaa ttaccgttca ttcccgacga cccgcgcgtc atcgattcaa caggcgccct
     3301 cgagctgggc ggggtcccga agcgcctgct ggtcatcggc ggcggcatca tcggcctcga
     3361 gatggccacg gtgtacgcag cactcggcgc gaagctctcg gtcgtcgagc tcacgggcca
     3421 gctcatgccg ggcgcggacg tcgacctggt gcgtccgttg gagaagcggc tcaaggctcg
     3481 ttacgagcag atcctgctga acaccaaagt cacgggctgc acggcgaagg cggaaggcct
     3541 ggtggtcgag ttcgaagacg ccaatggcaa acgtacggat accttcgatc gcgtgctggt
     3601 ggccgtgggg cgcacggcca atggcaagcg cattggtctc gagaacgcgg acatcgatgt
     3661 cagcgatcgc ggcgtgattc ccgtggacaa acagatgcgc acgaatctgc cgcatgtatt
     3721 tgccatcggt gatctcgtgc ccgggccgat gctggcacac aaggccatgc atgaggcgaa
     3781 agtggcggcg gaagttgccg cgggacacaa gagtttcttc gacgcgcgcg tgattccttc
     3841 ggtggcctat acggacccgg aggtggcctg ggtgggtctc accgaaaccg aggcaaagaa
     3901 aaacggcacg caatacaaga aaggtcagtt tccgtgggcg gccagcggcc gttcgctggc
     3961 gcttggccgc gacgaagggt tctccaaact actgttcgat ccggtttcgc atcgcgtgct
     4021 gggcggcggc atggtgggtc cgaatgcggg cgagctggtg gccgaagtgg cgctggccat
     4081 cgagatgggc gcggacgcgg ctgacatcgg cctgacgatt catccgcacc cgacgctgtc
     4141 ggagacggtg gcgctgtcgg ctgaggcctt cgaaggcacc atcaccgacc tctacatgcc
     4201 caggaaagct tagttagagt gctcctggct gctcacggac ggcgcacctt gggcgccgtt
     4261 ttccgatgtg gcgcgtgagc gcctgaagag catgagcacg cgctgactag aggtttgcgg
     4321 cgctgggggc ggcggcggtg agttcgcctg gcgagggacc ggcgaacgcg tattcaacga
     4381 tcacgcgaac ggccagcgtg tcgtccacgc tgaagttcgc gacatccagg cacaccgagc
     4441 gctggtctgc gctgaccgga tattccaccg gtgcggcggc gatgtgcagg ttgccgagcg
     4501 tcggcgcacc ggcatcccgt tccagccacg gggattcact tttcggcaca cgcgcctgca
     4561 cgcgcacatt ggtggcaagc gccgcggaag gaatggacgc acacagcgcg atcggcaccg
     4621 cgacgcggtc cgagcaggtc accgggccga cttcgtcggg atgcgcgctg accttgcagg
     4681 gcggcagatg tgccagcgac tcggcggagc ccgcctcgcc attgcgcgcg gggatcgcgg
     4741 cgccggatgc gtccacgccg ccgttcgtgg aacctgccgc ggactcggga ttctgccgcg
     4801 ctgcgagagc ggcaaactgc ttggtgaatt cgacctccat ggtcgccgcg agctcttcct
     4861 tggcgctgac cgcacgcaac gaggaccagg cgtacccgcc gacgatgcag ccgatcatca
     4921 acgccgcggt ggccagtacc gagcgaatgc gattcttctt cggcttcggg cgcccgtcgc
     4981 tgggcgcacg attgcgcacc agctgaacta tggccgtcag catggtcgcg agcgcgccaa
     5041 taatggccgc gatgatggtt tcggaaatac ccatcctggg aagcctctac aacctcaaac
     5101 aagcagggac gagtgtagtc cggccctgtc gcgcggcagc gcgggggcgt tcctttgtgg
     5161 gcactggttc acatttcgcc tcgcacaaat cgggggcggg cagcgaaacc ttcaggtgag
     5221 gccccgcagg cgttcgagat aggcgttttc gtcgggcggt ttgccgccgc gctgcgcctc
     5281 ccaaagttgt tcggccagga cttcgagcat gcggtgctcc gcctcatgcg cgccgcccaa
     5341 ctgccgggaa agctttgcgt ggaggtcggc aatgccagcg gggcggttgg tggccacctg
     5401 ctcgcggacg gcgagatgca ggcccatgtg cagccacgga ttgagctgcc cgctgcgcgg
     5461 ggaatagtta gcgaggtcgg cggcggccga ctccacgatg acgtggtatt ccgggtgctc
     5521 ggaaatgacc gccacgacct gccggtcgag cggtgacagc gtgtgctgct gcttgaaccg
     5581 gcgccatgcg gcgcgatagg tctcacgcag ctggtcgcgg gactggccgg cgaatatcac
     5641 ggagccttct tcgccgcggc gatgatcttg tggcgcaggg ggtattcgca gagtgtgttg
     5701 acgaggcacc gttcgcattc cggtctcagt gccttgcaga cgtagcggcc atgcaggatg
     5761 agccagtgat gagcatcgag cgcgaattcc ccgggcgtat gtttgaccag cgcatcttcc
     5821 acggcccgcg gtgtttcccc taccgcgatc cgagtc
//
LOCUS       DASQJS010000083        14548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_45800, whole genome shotgun sequence.
ACCESSION   DASQJS010000083 DASQJS010000000
VERSION     DASQJS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14548
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45800"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VEW08_03275"
     CDS             <1..402
                     /locus_tag="VEW08_03275"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYJ39784.1"
                     /translation="PLEGPWANILPVNPAYKWAGGGLLATPTDVARFGAAHWAPGALD
                     QRTLADLFTVQTQATDRSPPLGLGWRIDEDPNKRLRWHHAGGQDGARASLVVYPKEKL
                     AIAFATNLTGAPGDVNGPSAKIADALLEIRT"
     gene            511..1233
                     /locus_tag="VEW08_03280"
     CDS             511..1233
                     /locus_tag="VEW08_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HYJ39785.1"
                     /translation="MKTSSLRRLVALLGYVSCLAAMPLAAQVGPIQPVMVLPDGKLEV
                     THDRPSFDEDYSVPVDVYVAADGTVTNVVVTDSSKNLNADAVAADYMRSRKFLPALDD
                     KGQAVEGMAKVNVNMFKRGSKKVVRVTLKPPPMAVETERLRRMMCADFVWEVNRMQNE
                     AGVRDASVETMPYISAHMYAQGKDMAGDVAEKFWDTWSKTLHKVMERCEKSPEKMYFT
                     EVLVPALDGTMPTRETATAAAQ"
     gene            complement(1272..2189)
                     /locus_tag="VEW08_03285"
     CDS             complement(1272..2189)
                     /locus_tag="VEW08_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39786.1"
                     /translation="MPTKRITIYLRAAALFAAWVLCDSSALAETKPADPAAALRARYS
                     ALSQQLDNSAIQRGLIVESNESVTTPRGDAYAVIQYPFATVTAAFENPANWCDTMILH
                     LNVQYCRAKTGDGEPQLSTAVGKNINQPLDDTHRVDFIYKVTASTEDFLGVELVAKEG
                     PLGTSDYRISLELTALPDHRSFMHIQYSYTQGAMARYVSGVYFSTKGRDKVGFTLVQD
                     DDDEPRLVRGTRGALERNTMRYYLAFDAYLRSLSSPAPQRFEQSLEIWFADTERFARQ
                     LREVKHDDYMAMKRSQYQRQQTEAIKAGS"
     gene            complement(2259..2759)
                     /locus_tag="VEW08_03290"
     CDS             complement(2259..2759)
                     /locus_tag="VEW08_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39787.1"
                     /translation="MHRAACFTLLLALIVSHPVAADDKKFGTAIRDAIAKEVHERKEA
                     EASNAKDEQDLPVVANVGDRKRSVLHGVLWGGSDDSLRVATCPMGSAGAPTFDEVRNY
                     YRDYSDDEMWKICVNRSTYQGAIPGACACSLLAYPNPWRPVETTRQSEQPDEPIANPE
                     PEDSDK"
     gene            complement(2837..4873)
                     /locus_tag="VEW08_03295"
     CDS             complement(2837..4873)
                     /locus_tag="VEW08_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ39788.1"
                     /translation="MLRKFVLAALVLAYSLSASAQVSVTDLAKPPASATHYIIQSTGG
                     KHGDSWRWVGSDGVRMGRESINLRGQVFELDSSGKAGADGMPVSITIRGITPQGDASE
                     TFGIAGGKASWKSPVDAGSADYTKPAFYSAQGGPFDMSAWLLERLLASPDHTLALLPG
                     GKAKAEKLTTLTVGEGAAKKEITAWAVTGISNSPIPIWSDANGKFFGFSFFLSWLPEQ
                     YASEHVRITEAQAKAMAAQAPVLAKALVKTPGGAVAFTNVRVFDADAKMFLEDQTVIV
                     DKGLIAAVFPAREAKIPAGAQVIDGHGKTLVPGMWDCHMHVSSDYTGLQELSMGVTSL
                     RDPGNDDSQTIDRRVRAARGDLLFPHVYASSLIDGKGTYTAQVANVATSQAEAVSLVD
                     KAKAGGFIGVKFYGTLNKDWLPAAAAEAHKQGLHVHGHIPVGMRTLDAVNAGYDEVTH
                     INWIMMQAMPDAVIKESNGIQRFEGPGRYAKDVDINGKPLSDLISAMASKGIYSDPTM
                     VAFESLYVPENGDLSPSYAPFVGTLPPSTERGFRTGGFAVPKDLTRAHYRASWAKMVA
                     LLGKMHKAGVPIVAGTDGAGIELVHELEIYVEAGMTPGEALATATIIPARLVGQDKRT
                     GSIKPGKVADLVLVDGDPSKRVGDLRNTRVIMLDGKLLDADALRTAAGFSGRPK"
     gene            4959..5087
                     /locus_tag="VEW08_03300"
     CDS             4959..5087
                     /locus_tag="VEW08_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39789.1"
                     /translation="MRRLDLFAQFHQAVVATLVSANNPVDWGGGLLALLKAVGPGS"
     gene            5142..5528
                     /locus_tag="VEW08_03305"
     CDS             5142..5528
                     /locus_tag="VEW08_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39790.1"
                     /translation="MTSFRWQRVIAVLCAASLVLTSCYSLSPVPIPSGESPPALPAVS
                     VGDTVIITTRTNETKTFVVTAVEADALVGKRVRVVYTDMATLNVKHLRKGPTTWVVLG
                     VVFGILSIMAAVEAAEAYDDVFEGNY"
     gene            complement(5525..5917)
                     /locus_tag="VEW08_03310"
     CDS             complement(5525..5917)
                     /locus_tag="VEW08_03310"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HYJ39791.1"
                     /translation="MKDDIDAYAKKQKPKQKSVVDSLRKTIDAALPKATSKVWHGHPV
                     WFIGENPVVGYSTKTTGVALLFWNGQEFGEPALTPIGKFHAAELKYTDEADIDSKLLA
                     RCLRKSKTNIWDAVAWRRQAMAKSGSGN"
     gene            complement(5914..6357)
                     /locus_tag="VEW08_03315"
     CDS             complement(5914..6357)
                     /locus_tag="VEW08_03315"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYJ39792.1"
                     /translation="MKVMVIVKATKNSEAGVMPSEKLLTDMGNFNEQLVKAGIMQAGE
                     GLHPSAKGKRIQFSGGTHTVIDGPFAETKELVAGFWIWKVKSMDEAVSWARRCPDPMP
                     GEEATLEIRPIFEAEDFGEAYTPELQAKEARMRAELERQGKGPHA"
     gene            6547..7143
                     /locus_tag="VEW08_03320"
     CDS             6547..7143
                     /locus_tag="VEW08_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39793.1"
                     /translation="MRFSNVFLALAAATLIATGCAQQKAPANKALDAIEASLKDVRDD
                     AAKYAPDGLKGVEAQFARLKESYDKKEYDNVLAGTPELDKAVASLKDAVAAGKKHAAD
                     ALAAAKSEWENLNTEVPKMIETIQARVDEQSKKTFRLKVSKEELESAKSGLEWMKTEW
                     AEATAAVNSGKQVVAAEKARAVKAKGEEVQKTLSMKEA"
     gene            7143..7727
                     /locus_tag="VEW08_03325"
     CDS             7143..7727
                     /locus_tag="VEW08_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39794.1"
                     /translation="MRLSKSLVAIAATTILFTGCAKNEEPARQALAAAEASLAEVRVD
                     AAKFAPEELQVAEARLVKARENLAKEEYKDVLGDATQLSKETATLKEVVISKQTQVAA
                     ATHEWEALSEEVPKMVKAIEFRVDSLSGSKLPKDVNKEAFEAAKATLQSMKSMWAEAS
                     AAFNEGNAVAAADKARAVQAKGEEVIGQLAMSPV"
     gene            complement(7745..8698)
                     /locus_tag="VEW08_03330"
     CDS             complement(7745..8698)
                     /locus_tag="VEW08_03330"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYJ39795.1"
                     /translation="MAFCVAESGTRVPDLSDLETFVAVADAGGVSGAARRLGLPKSIV
                     SRRLSRLESELNTQLLTRTTRGAALTEAGATFREHAARVVAELEAARDSLSPEGELRG
                     LLRIAAPLSFGASHLAPLIAEFAKRHPQLQITTAYGDRFVDLVGEGFDVAIRLGWLPD
                     SSLVARRIGPVTGRLVATPDYVKTNGAPMSLDDIGNHPALMQGMEIWRVRDGDQVITL
                     HPQGRFKADHGQALVSAVLAGLGIAMLPDFLIDEHIASGALVTLLPQYPMPEAGLYVV
                     RPPGDHPSRKVRVFTDMLVDRLAAQCNVAPGKIVSRTPASI"
     gene            8766..9392
                     /locus_tag="VEW08_03335"
     CDS             8766..9392
                     /locus_tag="VEW08_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HYJ39796.1"
                     /translation="MSNILVINSSAAGRDSVSRVLVADAVSKLLEVNPHARVTHRDLG
                     ENPPPHLIAQTLNGVRGEAATEPEFAARRLSDELIGELKSADTVVIGAPMYNFSVPTT
                     LRAWFDHVLRAGVTFKYADGAVRGLLGGKRVIVIESRGGLYSEGPAQAVDFQEPYLRH
                     LLGFIGITDVTFVHAEKIGYGPEARTESLEAAKAKISEVVGAELVKAA"
     gene            9542..9799
                     /locus_tag="VEW08_03340"
     CDS             9542..9799
                     /locus_tag="VEW08_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39797.1"
                     /translation="MAEKPQPDPVPADDPPPHPAEPLQIDTPVIDMQDLEHKPGPDTR
                     VSSKGVRIEPTLGDDTHDFAREDEAALHAIEELEKYLKEAR"
     gene            9796..10152
                     /locus_tag="VEW08_03345"
     CDS             9796..10152
                     /locus_tag="VEW08_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39798.1"
                     /translation="MTSPKKVDVDKLLDEADGETPKGHDIRSLGPSDSSDSGADMVGV
                     GGLDSTTDRNGTGERTSVENDPDLTDADIAADDIARAEEVGLGGGLDQAEEAQLGVTD
                     EQIEAAVRERLGLDRG"
     gene            complement(10149..10691)
                     /locus_tag="VEW08_03350"
     CDS             complement(10149..10691)
                     /locus_tag="VEW08_03350"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ39799.1"
                     /translation="MPKTDLLEIRPIELADVPALLKIIADSRGEYGIAAKGVPLLEPA
                     DHALYATYQRQRTLYFVALLGGEVVGGAGVAPLAGADPLTCELQRMYLRRDARGRGIG
                     DALLERCLAAAKQFLYVRCYLETVTQMQAALEFYGRHGFRDLQAPIGHTGHEHNDRWM
                     MRTLRASAREYPGEAARAYG"
     gene            complement(10914..11618)
                     /locus_tag="VEW08_03355"
     CDS             complement(10914..11618)
                     /locus_tag="VEW08_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39800.1"
                     /translation="MHFGPTFYRTAAICSVLSAVTTLGLIFLPNFFTPVDGFEQRMAR
                     VHDPAYVLRSWVYFIHPFLVMMAGLAIAVRIRSIAAAFAVVGLLGFVLWGFTEAAQQT
                     MTLFAFDKWRTAFATADVATRADIRTLTMMYDGLWDGMYFLILVGFAIGNFCLSVVLL
                     HLHGLARVTGLFMFAAVLLTLTLIARELGWFSLPEPLASWSYPAIQPLGRVLIGVWLW
                     RMADESVPLWRPAIPT"
     gene            complement(11735..12079)
                     /locus_tag="VEW08_03360"
     CDS             complement(11735..12079)
                     /locus_tag="VEW08_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39801.1"
                     /translation="MKTRHWLIALGAMVALHADVYGLTPPSASDGPVRLLACVVTPQG
                     ILEAEVDNQTEDSMECSIRCNYEFGDKMLSQSFNVTIPGRFHGRVGRVDTTAARAGNY
                     SGDLGDCKKSPR"
     gene            complement(12087..>14548)
                     /locus_tag="VEW08_03365"
     CDS             complement(12087..>14548)
                     /locus_tag="VEW08_03365"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYJ39802.1"
                     /translation="AALLCSLCAATITHGATLPTGFAETRVATGMSSPTAMAVAPDGR
                     IFVAQQGGALRVVKNGALLTQPFLTASVNASGERGLLGVAFDPNFASNSFVYVYYTTT
                     TSPIHNRVSRFTASAANPDVAAAGSEVQILNLPNLSSATNHNGGAIHFGTDARLYIAV
                     GDNANSSNAPSLTTTLGKMLRINPDGTIPSDNPFISQTTGMNQAIWARGLRNPFTFAI
                     DPTNGRMHINDVGEVTWEEVNQGVAGSNYGWPQTEGNNPAGVAGVRYPIHTFNHSGGV
                     CAITGGAFYRPTTATFPAEYAGRYFFGDFCAGFIRTLSPPNYNTPTNFATGVSSLADI
                     QVHQDGSLYYLARGGGGELFRVQFTANTAPSISAHPANVTVAAGQSATFAVTASGTAP
                     LAYQWQRNGVNIAGATARTYTRVTTAADNGSMYRVLVSNASGSALSNSATLTVLSNSA
                     PTGTITAPANGANYRGGQVITFAGTGNDPEDGNLPPSAFTWRVDFHHDDHVHPHMGDT
                     SGISTGTFTIANRGETSSNVFYRVILTVRDSSGLTHTSQSDVRPLTSVIRIESNVANA
                     QLTLDGLPITAPFSFTGVEGVIRTLGVVTPQTSGGTTYDFVSWSDGGQATHEITTPND
                     DTTFTALFQPAVNRTVFSDNFEAAGGWTLTSGANTAPRGRWARGDPQSTNFNGVTLQL
                     GTCDGPSVNCFITGLSAGSAVGSGDVDEGMTSVQSPAIALPAGGTTTLRFRYYLAHNN
                     NATSADYFRVRVVGTSGTPQTVFTRPGTGGTVVGGAWTTQTVNISTFAGQTVRLRFEA
                     ADAAAGSIIEAGFDNVSVTRQ"
BASE COUNT         2685 a         4601 c         4628 g         2634 t
ORIGIN      
        1 ccgctcgaag gcccgtgggc caatattctt cccgtcaatc ccgcgtacaa atgggcgggc
       61 ggcgggttgt tagccacgcc gaccgatgtt gcgcgtttcg gcgccgcaca ttgggcgccg
      121 ggtgcgctcg accagcgcac gctcgctgac ctgttcactg ttcagacgca ggcgaccgac
      181 aggtcgccgc cgctcggcct cggctggcgc atcgacgaag acccgaacaa gcggctgcgc
      241 tggcaccatg ccgggggcca ggacggcgcg cgcgccagcc tcgtcgtgta tccgaaagag
      301 aagctggcga tcgcgttcgc gaccaacctc accggggctc cgggcgacgt gaatggaccg
      361 agcgcgaaga tcgccgatgc cttgttagag atacgaacat aacgggccac ctgttatttc
      421 gctcacagtc cgggggtcgc cctagggcgg accgcacagt attccggcaa ttcgattgcc
      481 ggcagccggc gcgcgaaata gtttcgagcc atgaaaactt caagccttcg tcgactggtc
      541 gcactgcttg gttatgtttc atgcctcgca gccatgccgc tcgccgccca ggtgggcccc
      601 atccagcccg tgatggtgct tcctgacggc aagctagagg tcactcacga ccgcccgagc
      661 ttcgacgaag actattccgt ccccgtcgac gtgtacgtcg ccgccgatgg caccgtgacc
      721 aacgtcgtgg tgacagatag cagcaaaaat ctcaacgccg atgccgtggc cgccgactac
      781 atgcgcagcc gcaagtttct tccggcgctc gatgacaagg gccaggccgt cgaaggcatg
      841 gccaaagtca atgtgaacat gttcaagcgc gggtcgaaga aagtcgtacg cgtcactctc
      901 aagccgccac cgatggcggt cgagaccgaa cgcctgcgcc gcatgatgtg cgcggatttc
      961 gtgtgggaag tgaaccgcat gcagaacgag gccggcgtgc gcgacgcaag cgtcgaaacg
     1021 atgccctaca tatcggcgca catgtatgcg cagggcaagg acatggcggg tgacgtcgcc
     1081 gagaaattct gggacacctg gtcgaagact ctgcacaagg tgatggagcg ctgcgagaag
     1141 tcacccgaga agatgtattt caccgaggtg ctggtgcccg cgctcgacgg cacgatgccg
     1201 acgcgcgaga cggcgaccgc cgccgctcaa taacattgcg ccgcgctgcg tggcgcatct
     1261 cgagaaaatc ctcaagaccc agccttgatc gcctcggtct gctgcctctg gtactggctg
     1321 cgtttcatcg ccatgtagtc gtcgtgcttc acttcccgca gctggcgcgc gaagcgttcc
     1381 gtatcggcaa accagatctc gaggctctgc tcgaatcgct gcggcgccgg gctgctcagg
     1441 gagcgtagat aggcgtcgaa ggcgaggtag taacgcatgg tgttccgctc cagcgcgcca
     1501 cgagtgccgc gcaccaggcg cggctcatcg tcatcatcct gcaccagcgt gaagccgact
     1561 ttgtcccggc ctttggtgga gaagtagacg ccgctgacgt agcgcgccat cgccccctgc
     1621 gtgtacgaat actggatgtg catgaaggag cggtggtccg gcaacgcggt cagttccagc
     1681 gaaatacggt agtcgctggt gccgagcggg ccctctttgg ccacgagctc cacgcccagg
     1741 aagtcttcgg tcgaagcggt gaccttgtag atgaaatcga cacgatgcgt atcgtcgagc
     1801 ggctggttga tgttcttgcc caccgcggtc gacagctgcg gttctccgtc gcccgtcttg
     1861 gcgcggcaat actgcacgtt gagatgcagg atcatcgtgt cgcaccagtt cgccggattt
     1921 tcgaacgcgg cagtgacggt ggcgaagggg tattgaatca ccgcataggc gtcgccacgc
     1981 ggcgtggtta cgctttcgtt ggattccacg atcaacccgc gctgaattgc actgttgtcg
     2041 agctgctggc tcaaagcgga ataacgcgcg cggagcgccg cggcgggatc ggctggtttc
     2101 gtctcagcca gagcgctcga atcgcacagg acccacgcgg cgaaaagcgc tgcagctcga
     2161 aggtaaatgg tgattcgttt ggttggcaaa ggcacaagct cttgccaatg agtgtccggg
     2221 caggcgcgcg tgcgggctat aggcgcatgc cgcaagactc atttatcgct gtcttcaggc
     2281 tccggattcg cgattggctc gtcgggttgc tcgctctggc gcgttgtttc cacggggcgc
     2341 cagggattcg ggtacgccag aagcgagcag gcgcaagcgc ccgggattgc tccctggtag
     2401 gtgctgcgat tcacgcagat cttccacatc tcgtcgtcgc tgtagtcgcg atagtagttg
     2461 cgcacttcgt cgaaggtggg cgcgcccgca ctgcccatcg gacaggttgc gactctcaat
     2521 gaatcgtcgg agccgcccca gagaacgcca tgcaggacgc tacgtttgcg atcgccaaca
     2581 ttcgcgacca cgggcagatc ctgctcgtcc ttggcgttgc tggcttctgc ttccttgcgc
     2641 tcgtgaactt ccttggcgat cgcatcgcgt atcgcggtgc cgaatttctt gtcatcggcg
     2701 gcgacgggat gcgacacgat gagcgcaagc agcaaggtga aacaggcggc gcggtgcatg
     2761 gcgccgaatc taatcctcga cggcgatccg ggcaatccgt tgattgccac gaaccgccgt
     2821 tcaggttccg ttcagcttac ttcggccgcc ccgagaagcc cgccgccgtg cgcagcgcgt
     2881 cggcatcaag tagtttgccg tcgagcatga tcacgcgggt attgcgcagg tcgcccacgc
     2941 gcttcgaggg atcgccgtcg accagcacca ggtccgcgac cttgccgggc ttgatggagc
     3001 cggtacgttt gtcctggccc acgagccttg cgggaatgat ggtcgcggtg gccagcgcct
     3061 cgccgggggt catgcccgcc tccacgtaga tttcgagctc gtgcacgagc tcgatgcccg
     3121 cgccatccgt gcccgcaacg atgggcacgc cggctttgtg catcttgccg agcagcgcaa
     3181 ccatctttgc ccagctcgcg cgatagtgcg cgcgcgtcag gtccttcggc acagcgaaac
     3241 cacccgtgcg gaaaccgcgc tcggtggacg ggggcagcgt gccgacaaac ggcgcatagg
     3301 atggcgacag atcaccgttc tccggcacgt acagggattc gaacgcgacc atcgtcgggt
     3361 cgctgtagat gcccttggac gccatcgccg agatcaggtc cgagagcggc ttgccgttga
     3421 tgtcgacatc cttcgcataa cgtcccgggc cttcgaaacg ctggatgccg ttcgattcct
     3481 tgatcactgc atcgggcatc gcctgcatca tgatccagtt gatgtgcgtg acttcgtcgt
     3541 agcccgcgtt caccgcatcg agcgttcgca tgccgaccgg aatgtggccg tgcacgtgca
     3601 gaccttgctt gtgcgcttcg gcggccgctg ccggcagcca gtccttgttc aacgtgccgt
     3661 agaacttcac gccgatgaag ccacccgcct tcgccttgtc gacgagcgag acggcttccg
     3721 cctggctggt tgcaacgttt gccacctgcg ccgtgtatgt acccttaccg tcgatgagcg
     3781 atgacgcata cacgtgcggg aacagcaggt cgccgcgtgc cgcgcgcaca cgccggtcga
     3841 tagtctgcga gtcgtcgttg cccgggtcgc gcagtgaggt gacccccatc gagagctcct
     3901 gcagcccggt gtagtcggac gacacgtgca tgtggcagtc ccacatgccc gggaccagcg
     3961 tcttgccatg gccgtcgatg acctgcgcgc ccgccggaat cttggcttcc ctggccggga
     4021 ataccgcggc gatgagcccc ttgtcgacga tgactgtctg gtcttcgagg aacatcttcg
     4081 cgtccgcatc gaacacgcgc acgttcgtga acgccacggc gcctcccgga gttttcacca
     4141 gcgcctttgc aagcaccggt gcctgcgctg ccatcgcctt cgcttgcgcc tcggtgatgc
     4201 gcacgtgctc cgacgcgtat tgctccggca gccagctcag gaagaacgag aaaccaaaga
     4261 atttgccgtt ggcatccgac cagatcggaa tcggggagtt gctgatgccg gtgaccgccc
     4321 aggcagtgat ctcctttttc gccgcgccct cgcccaccgt gagggtagtt agcttctccg
     4381 ccttcgcctt gccaccgggc agcagcgcga gagtgtgatc cgggcttgca agcaggcgct
     4441 cgagcaacca cgccgacatg tcgaacggcc cgccctgcgc ggaatagaac gccggcttcg
     4501 tgtagtccgc gctgcccgcg tccaccgggc tcttccagct ggccttgccg ccggcgatgc
     4561 cgaaggtttc actcgcatca ccctgcgggg tgatgccgcg aatcgtgatc gacaccggca
     4621 tcccgtccgc gcccgccttg ccgctcgagt ccagctcgaa cacctggccg cgcagattga
     4681 tgctttcgcg gcccatgcgt acgccgtccg agccgaccca ccgccaggaa tctccgtgct
     4741 tgccgcccgt ggactggatg atgtagtgcg tcgcgctagc cggcggcttg gcgagatctg
     4801 tcaccgaaac ctgcgccgaa gcagagagag agtaggcgag caccagggcg gcgagaacga
     4861 acttgcgcaa cacggatttc tcccaggagt gaattgggac cgagaatagt cgcttgagag
     4921 gtctcttgca ctttgtgcgc acacggcgcg gcgcagttat gcgtcgcctc gacctttttg
     4981 cccagtttca ccaggcagtc gtcgcgaccc tggtctcggc taacaatccc gtcgactggg
     5041 gcggcggtct gctggcgctg ctgaaagcgg tgggccccgg gtcgtagatg gattgagaag
     5101 ttaggcccgc cagagtagcc tccgtcgcca gggggttggc aatgacttca tttcgatggc
     5161 aacgtgtaat cgcggtcctg tgtgcagcga gtttggtgct gacgtcgtgt tattcactga
     5221 gtcccgtccc aattccgagc ggcgaaagtc cgccagccct gcctgcagtc tcggtgggcg
     5281 acaccgtcat catcaccacc aggaccaatg agacaaagac ctttgtcgtc actgcagtcg
     5341 aggcagacgc cctcgttgga aagcgcgtgc gcgtggtcta cacggacatg gccaccctga
     5401 acgtcaaaca ccttcgcaaa gggcccacaa cctgggtggt gctgggggtg gtgttcggga
     5461 ttctctccat catggcggcc gtggaagcgg cggaagcgta tgacgatgtt ttcgagggga
     5521 attactagtt cccgctgcca ctcttcgcca ttgcctgcct gcgccaagcc accgcatccc
     5581 aaatgttcgt cttcgacttc ctgaggcagc gggctaacag cttcgagtcg atgtccgcct
     5641 cgtccgtgta cttcagctcc gccgcgtgga acttgccgat gggcgtcagc gctggctcac
     5701 cgaattcctg accgttccag aacagcagcg ccacgccggt ggtcttcgtc gagtagccca
     5761 ccacggggtt ctcgccgatg aaccacaccg gatggccatg ccagaccttc gaggtcgcct
     5821 ttggcagcgc cgcatcgatg gtcttgcgca gactgtcgac gacggacttc tgtttcggct
     5881 tctgcttctt cgcgtaagcg tcaatgtcgt ctttcatgcg tgtggtcctt tgccctgccg
     5941 ttctagttcc gcgcgcatgc gcgcttcctt cgcctgcagt tccggcgtgt acgcttcgcc
     6001 gaagtcttcg gcttcgaaga tcgggcgaat ttcgagtgtc gcctcttcgc ccggcatcgg
     6061 gtccgggcag cggcgcgccc aggacaccgc ctcgtccatc gacttcactt tccagatcca
     6121 gaatcccgcg acgagttcct tggtttccgc gaacgggcca tctatcacgg tgtgggttcc
     6181 gcccgagaac tggatgcgct tgcccttcgc gctggggtgc aggccttcgc ccgcctgcat
     6241 gatgccggcc ttgaccagtt gctcattgaa attgcccatg tccgtgagca gtttctcgct
     6301 cggcatgacg ccggcttcgc tgtttttcgt ggctttcacg atcaccatga ctttcatctc
     6361 gatctcctcg aattggatgt tcgaaaggta gtcgaacggc gcctcgggaa ttcgactgcg
     6421 ttcgtaagcc cttgatgtac ccaaggatcg cggcactttg cctaccgatc tcgcgccgat
     6481 gtcctacgcc tccaccagcc cccacgcgcc taccctagca atgttcaaaa accgatgggg
     6541 tgttctatgc gtttctccaa tgttttcctg gcgctcgccg ctgccacgct gatcgcgacc
     6601 ggctgcgccc agcagaaagc gcccgcgaac aaagctcttg atgcgatcga agcatcgctc
     6661 aaggacgtca gggacgacgc tgcgaaatat gcgcccgatg gactgaaggg tgtcgaagcg
     6721 cagtttgcgc gactcaaaga gagttacgac aagaaggagt acgacaacgt gctcgccgga
     6781 acgccggaac tcgacaaggc agtcgcctcg ctcaaggatg ccgtcgccgc tggcaagaag
     6841 cacgctgccg acgcgctcgc cgctgccaag agcgaatggg aaaatctgaa taccgaagtg
     6901 ccgaagatga tcgagaccat ccaggcccgc gtcgacgagc aatcgaagaa gacgtttcgc
     6961 ctgaaggtca gcaaggaaga actcgagtct gccaagtccg ggctcgagtg gatgaagacc
     7021 gagtgggccg aagccaccgc ggcggtgaac tcgggcaagc aggtcgtcgc tgccgagaaa
     7081 gcgcgcgccg taaaggcgaa aggcgaagaa gtgcagaaga cgctgagcat gaaggaggcg
     7141 taatgcggtt atcgaagtca ctcgtcgcga tcgccgcgac cacgatcctc ttcaccggct
     7201 gcgccaaaaa tgaagagccc gcacggcagg cgttggcggc agccgaggcg tcgctcgccg
     7261 aagtgagagt cgacgcggcg aagttcgcgc ccgaggaact gcaggtcgcc gaggccaggc
     7321 tggtgaaggc cagggaaaac ctcgccaagg aagagtacaa agatgtgctc ggtgacgcga
     7381 cgcagctcag caaggaaacc gccacgctca aagaggtggt gatctcgaag cagactcagg
     7441 tcgcggccgc cacgcacgag tgggaagcct tgagcgaaga agttcccaag atggtcaagg
     7501 ccatcgagtt ccgcgtcgat tcgctgtcgg gatcgaagct gccgaaagac gtgaacaagg
     7561 aagcattcga ggccgccaag gccacgctcc aatcgatgaa gtcgatgtgg gccgaagcca
     7621 gcgctgcgtt caacgagggc aacgcggttg cggccgcgga caaggcccgc gccgtgcagg
     7681 ccaagggcga ggaagtcatc ggccagctcg ccatgagccc ggtctgaact gccgcactga
     7741 ttagctagat tgacgctggc gtccgggaga ctattttccc gggcgccacg ttgcattgcg
     7801 cggccagccg gtccaccagc atgtccgtga acacacgcac tttacgtgac gggtgatcgc
     7861 ccggcggcct caccacatag agtcccgcct ccggcatcgg gtattgcggc aggagcgtga
     7921 ccaacgcgcc cgaggcgatg tgctcgtcga tgaggaagtc cggcagcatc gcaatgccca
     7981 gccccgcgag caccgccgac accagcgcct gaccatggtc cgccttgaag cgtccctgcg
     8041 ggtgcagcgt gatgacctga tcaccatcac gtacgcgcca gatttccatg ccctgcatga
     8101 gcgccggatg atttccgatg tcatcgagtg acatcggggc gccgttcgtt ttcacgtagt
     8161 cgggagttgc caccagcctg ccagtcaccg gcccgatgcg ccgcgcgacg agtgatgaat
     8221 ccggcagcca ccccaggcgg atcgccacat cgaatccctc gcccaccaga tccacgaagc
     8281 gatcaccgta ggccgtggtg atctgcagct gcggatgtcg ttttgcaaat tccgcgatca
     8341 gcggcgcgag atgcgacgcg ccaaatgaca gcggcgccgc gatgcgcagc agacctcgca
     8401 gttcgccctc tggggaaagg ctgtcgcgcg cagcttcgag ctccgccacc acgcgcgccg
     8461 cgtgctcgcg gaacgtcgcg ccggcttccg tgagtgccgc cccgcgggtc gttcgcgtga
     8521 gcagttgggt gttgagctcg ctctcaaggc gactgagcct tcggctcacg atcgacttcg
     8581 gcagccccag ccgtcgcgcc gccccggaca caccgccggc gtcggcaaca gccacaaaag
     8641 tctccaaatc ggataaatcc ggcactcgcg ttccactttc agcaacacag aaggccattc
     8701 gacgcggcta gtgtcgcgct attggcaacg cgaagatggg ctccaagaga aaaggagtca
     8761 agcccatgtc gaacattctc gtcatcaata gcagcgccgc aggccgggat tcggtctcgc
     8821 gtgtgctcgt cgccgacgcc gtcagcaagc tgctcgaggt caatccacac gccagggtca
     8881 cgcatcgcga cctcggcgag aatccgccgc cgcacctgat cgcgcagacg ctgaatggcg
     8941 tgcgcggaga ggccgcgacc gaacccgagt tcgcggcgcg acgcttgtca gatgaactca
     9001 tcggcgagct caagtccgcc gacaccgtcg tcatcggcgc acccatgtac aacttcagcg
     9061 tgcccaccac gttgcgcgcc tggttcgatc acgtgctgcg cgccggcgtc acgttcaagt
     9121 acgccgacgg cgccgtccgc gggttgttag gcgggaagcg cgtgatcgtc atcgagtcgc
     9181 gcggtgggct ctacagcgaa ggcccggcgc aggccgtgga cttccaggag ccgtacctgc
     9241 ggcatctgct cggcttcatc ggcatcaccg atgtgacgtt cgtgcatgcc gagaagatcg
     9301 ggtacgggcc ggaggcgcgc accgagtcgc tggaagcggc gaaggcgaag atctcggaag
     9361 tcgtgggcgc ggagctcgtg aaggcggctt gagagcgccg agcgctcgat tcgcaccagc
     9421 cgcgcgatca atgtgattat tgcttgcgcg gcccctcaat gatttgcgcc ctgcacccgc
     9481 ctacacggcc gtgcggcgag cgctgatgct tgcgcgccgg tccgcgcgcc caagcttggc
     9541 aatggccgaa aagccccaac ccgatccggt gcctgccgac gacccgccgc cccacccggc
     9601 cgaaccgctg cagatcgata cgccggtcat cgacatgcag gatctcgagc acaagccggg
     9661 tccggatacg cgcgtgagca gcaagggtgt gcgaatcgaa ccgacgctcg gcgacgacac
     9721 ccatgatttc gccagggaag acgaagcggc actccatgcc atcgaggaac tcgagaaata
     9781 tctcaaggag gcacgatgac gagcccaaag aaagtcgatg tcgacaagct gctcgacgaa
     9841 gccgacggtg aaacaccgaa gggacacgac atccggtcgc tcgggccgag cgacagctcg
     9901 gacagcggag ccgacatggt cggcgtcggt ggcctcgaca gcaccaccga tcgcaacggc
     9961 actggcgagc gtaccagcgt ggaaaacgat cccgacctta ccgacgcgga catcgccgcc
    10021 gacgacattg cgagggccga ggaagttggc ctcggtggcg gactcgatca ggccgaagag
    10081 gcgcagctcg gagtcacgga cgaacagatc gaggccgcag tccgggagcg actgggcctc
    10141 gatcgtggct agccatacgc acgcgccgct tcgcccggat attcccgagc cgatgcgcgc
    10201 agcgtgcgca tcatccagcg atcattgtgc tcatgaccgg tgtgcccgat cggtgcctgc
    10261 agatcgcgaa aaccatggcg gccgtagaat tccagtgcag cctgcatctg cgtgaccgtt
    10321 tccaggtagc accgcacgta gaggaactgc ttcgcggccg cgaggcagcg ctcgagcagc
    10381 gcgtcgccga taccgcggcc gcgtgcatcc ctgcgcaggt acatgcgctg cagctcgcag
    10441 gtcaggggat ccgcgcccgc aagtggcgcc acaccggctc cgcccaccac ctcaccacct
    10501 agcaaggcga cgaagtacag cgttcgctgc cgctggtagg tggcgtagag cgcatgatcc
    10561 gcgggctcga gaagtggaac acctttggca gcgatcccgt attccccgcg cgaatccgcg
    10621 atgatcttca gcagcgccgg cacgtccgcc agctctattg ggcgtatttc gagtagatcg
    10681 gtctttggca tgttcgcgtc cttgaagggg ccttcccggc ggatgcgaac aaaaaaacgc
    10741 ctccgctagg gaggctggtt tcggttgagc tgaatggtca gtttcgtgcg ctcaatcacc
    10801 ggcctccacg ttgagtggtt gccacgaata cgatttgcag aaatggttga gcacgggagg
    10861 gaagttacgt gagtgcgcga ggggctgtca acgcgcgatg agggatcgat tggttatgtc
    10921 ggtatggcgg gacgccacaa tggcaccgat tcgtctgcca tgcgccacaa ccacacaccg
    10981 atgaggacgc ggccaagtgg ttgaatcgcc gggtagctcc agctcgccag cggctcgggc
    11041 agcgagaacc agccgagttc ccgggcgatg agcgtgagcg tcagcagcac cgccgcgaac
    11101 atgaacaagc cggtcacgcg cgcgaggccg tgcaaatgga gaagcacgac actcaggcag
    11161 aaattgccga tcgcgaagcc caccaggatc aggaaataca tcccgtccca caggccgtcg
    11221 tacatcatgg ttagtgtacg aatgtcagcg cgcgtcgcga cgtcggccgt cgcgaaggcg
    11281 gtgcgccatt tatcgaaggc gaacagcgtc atcgtctgtt gcgcagcttc ggtgaatccc
    11341 cagagcacga agccgagcag cccaactact gcgaaggcgg cggcgattga gcggatgcgc
    11401 acggcgatcg cgaggccggc catcatcacg aggaacgggt ggatgaaata cacccacgaa
    11461 cgcagcacgt aggccggatc gtgcacgcgc gccatgcgtt gttcgaatcc gtcgacgggc
    11521 gtgaagaagt tgggcaggaa gatcagcccc agcgtcgtta ccgccgagag caccgagcag
    11581 atggcggcgg tgcggtagaa agtggggcca aaatgcattg ccagattctt gccgagacgc
    11641 gagcggcact ccagccgttt ctcaacgcct gggcgtgttc cggcctgtgt tcggcgcgca
    11701 agagcgagca gaccctcgtt tcaatcctgg aacctcaccg aggactcttc ttgcagtcgc
    11761 ccagatcgcc ggagtagttg cccgccctgg cggcggtcgt atcgacgcgc cccacgcgcc
    11821 cgtgaaaccg cccggggatc gtgacgttga agctctggga aagcatcttg tcgccgaact
    11881 cgtagttgca gcgaatgctg cactccatgg agtcctcggt ctggttgtcc acctccgcct
    11941 cgagaatgcc ctgcggggtg acgacgcacg ccaatagtct gacggggccg tccgaggcgg
    12001 atggcggcgt caaaccgtag acgtctgcgt gaagcgccac catcgcgccg agtgcaatca
    12061 accaatgtcg cgttttcatg ctctccttac tggcgcgtca cactcacatt gtcgaagccc
    12121 gcttcgatga tgctgcccgc ggccgcatcg gccgcttcga agcgcagccg caccgtttgc
    12181 ccggcgaagg tactgatgtt caccgtctgc gtcgtccagg cgccgccgac cacggtgcca
    12241 cccgtgcccg gacgcgtgaa cactgtctgc ggagtgccgc tggtcccaac gacgcgaacg
    12301 cggaagtagt ccgcgctggt cgcattgttg ttgtgcgcca ggtagtagcg gaagcgcagg
    12361 gtggtggtgc ctccggccgg cagcgcgatg gcaggcgatt gaaccgatgt catgccttca
    12421 tcgacatccc ccgatcccac cgccgagccc gccgaaagcc cggtgatgaa acaattcacc
    12481 gacggaccgt cgcaggttcc gagctgcagc gtgacaccat tgaagttagt cgactgcggg
    12541 tcgccgcgcg cccatcggcc tcgaggcgcg gtgttggcgc ccgaggtgag cgtccatcct
    12601 cccgctgcct caaagttgtc gctgaacact gttctgttca ccgcgggctg gaacaacgcc
    12661 gtgaatgtcg tatcgtcgtt tggcgtcgtg atctcgtgcg tcgcttgccc gccatcggac
    12721 caggacacga agtcgtacgt ggtgccgccc gacgtctgcg gcgtcaccac tccgagcgtg
    12781 cgaatcacac cctcgacgcc cgtgaacgag aacggcgcgg taatcggcaa gccatcgagc
    12841 gtaagctgtg cgttggccac gttgctctcg atgcgtatca ccgatgtcag cggccgcacg
    12901 tcggactgcg aggtgtgcgt gagcccggac gaatcgcgaa ccgtgaggat cacgcgatag
    12961 aacacgttcg acgacgtctc gccgcgattg gcgatcgtga acgtacccgt cgagatgccg
    13021 ctcgtgtcac ccatgtgcgg atgcacgtga tcgtcatgat ggaagtccac gcgccaggtg
    13081 aaagcgctcg gcggcaggtt gccgtcttcc ggatcgttgc ccgtgccggc gaacgtgatg
    13141 acctggccac cgcggtagtt ggcgccgttg gcgggcgcgg tgatcgtgcc cgtgggcgcg
    13201 ctgttgctga gcaccgtgag cgtcgccgaa ttgctgagcg cggagccgct ggcattgctt
    13261 acgagcacgc ggtacatcga gccgttgtcc gcggcagtcg tcacccgcgt gtacgtgcgt
    13321 gccgtggcgc ccgcgatgtt cacgccattc cgctgccact gataggcgag cggcgccgtg
    13381 cccgaggccg tgaccgcgaa tgtcgcggac tggcccgcgg cgaccgtgac attggcaggg
    13441 tgcgccgaga tgctgggcgc cgtgttcgcg gtgaactgca cgcgaaacaa ttcgccgccg
    13501 ccaccgcgcg cgagatagta gagcgagccg tcctgatgca cctggatgtc cgccagcgag
    13561 ctgacgccgg tggcgaaatt ggtgggtgtg ttgtagttcg gtgggctcag ggtccggatg
    13621 aatcccgcgc agaagtcgcc gaagaagtag cgcccggcat actcggcagg gaacgtggcg
    13681 gtggtgggcc gatagaacgc cccaccggta atcgcgcaaa cgccgcccga atggttgaag
    13741 gtgtggattg gatagcgcac acccgcgacg cccgccgggt tgttgccttc ggtctgcggc
    13801 catccgtagt tcgaacccgc gacgccctgg ttgacctctt cccacgtgac ctcgcccaca
    13861 tcgttgatgt gcatgcggcc gttcgtcggg tcgatcgcga acgtgaacgg attgcgcagt
    13921 ccccgcgccc agatcgcctg gttcatgccc gtggtctggc tgatgaacgg attgtcggac
    13981 gggatcgtgc catccgggtt gatgcgcagc atcttgccga gcgtggtggt gagcgatggc
    14041 gcgttggaac tgttggcgtt gtcacccacc gcgatgtaga gcctggcgtc agtgccgaag
    14101 tgaatcgcac caccgttgtg gttggtcgcc gagctcaggt tcgggagatt caggatctgc
    14161 acctcgcttc ccgccgccgc gacgtcggga ttcgcggcgc tggcagtgaa gcggctcacg
    14221 cgattgtgga tgggcgaggt cgtggtcgtg tagtacacgt agacgaagct gttggacgcg
    14281 aagttcggat cgaacgccac gccgagcagg ccgcgttcac ctgaagcgtt cacgctggcc
    14341 gtgagaaacg gctgcgtcaa cagcgcgcca ttcttcacta cgcgtaaggc gccgccctgc
    14401 tgcgccacga aaatgcgtcc gtcgggcgcg acagccatcg ccgtcgggct ggacatgccc
    14461 gtggccacgc gcgtctcggc gaagccggtt ggcaacgtgg cgccatgcgt gattgttgcc
    14521 gcgcacagcg agcacaacag agctgcaa
//
LOCUS       DASQJS010000084         4790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_47975, whole genome shotgun sequence.
ACCESSION   DASQJS010000084 DASQJS010000000
VERSION     DASQJS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47975"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VEW08_03370"
     CDS             complement(<1..718)
                     /locus_tag="VEW08_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HYJ39803.1"
                     /translation="MAAGSLTSSLAFANHHIKIPLGIQLWTVKSEAEKDLEGTLRKVY
                     AAGFRDIEFAGYYGKAPADLAKLMKDIGFSLVSTHAGAGDIAKSGDKIIADAKALGLK
                     YVVCSSPGVSPEKEKLPWEERMKAVDLKDWKWNADLFNKFGKQVADAGMVFGYHNHSA
                     EFKKFDGKTAFDHLFAMTDPKFVKIEMDIGWVAVAQQDPVALLNQYKDRVIALHVKDV
                     AKRAADGKDPPSVALGEGTVD"
     gene            complement(823..3429)
                     /locus_tag="VEW08_03375"
     CDS             complement(823..3429)
                     /locus_tag="VEW08_03375"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ39804.1"
                     /translation="MDARRSLNPNREMLAVALLLLMAMVAVMQSASSDFPALHSILDT
                     AVFMVSGVLAFLLWDLGWRTGQSRPRLEAVCFTAVSILELLHVITALDFAEAPDAALL
                     LRLGTWSPAAYLLPLGLLAALPLSRRNTNPALLAVALVFISAAMMVLFAVVPRYSPPW
                     FLGITRPTLIPAALLWIPVIVGYWRLRHQDRLAKVFAVFAAVTFFVPLLILYSTGPAD
                     KMAILTHFVRVAGELYLLLSLTQMGTFDTAQRMTAERELKASNEALESRVAERTAALE
                     SANTDLRTEAHTRQVAERRALVQLERLALLERITRAIGERQDLESIFQVVVRSVEEHL
                     PADFSALCNYTHHTRVLTVCCVGERSGPLALELAMTEQSRIEIDQNGLSRCVAGKLVY
                     EADISKVRFPFPQRLAKGGLGSMVVVPLMVEQRSGVFGVLVVARHAPHAFSSAECEFL
                     RQLCDHVSLAANQAQLHDSLKQAYDDLQVSQGAVMQQERLRALGQMASGIAHDINNAI
                     SPVSLYVEALLTHETGFSARARGQLEIIQRAVDDVAHTVARMGEFYRQGKGYGQMELA
                     PVSVTQAFREVLELTRARWSDMAQQRGKVIETKVECEGESPVVMALASELREALINLV
                     LNATDAMPEGGTLTLRAGHSLEAGKGVSRRVFIEVSDTGAGMDEATRRRCLEPFFTTK
                     GDRGSGLGLAMVYGIAQRHGADIDIFSAPGEGTTFRLTFPSTSPAAQSTGSLRIQKIP
                     RHTKILLIDDDPVLLTSLREVLVHDGHQVQTASGGRVGIDAFRDALKAGKPFPVVITD
                     LGMPHVDGRAVAAAIKAAAPSTSIIMLTGWGQRLVASGETPADVFAVVSKPPRIAELR
                     QVIAECTGQPDT"
     gene            complement(3513..>4790)
                     /locus_tag="VEW08_03380"
     CDS             complement(3513..>4790)
                     /locus_tag="VEW08_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ39805.1"
                     /translation="RDVGGFSESDCEFLRQLSEHVALATNHAKLYADLQSAYDDLRLS
                     QQRITQHERLRALGQMASGIAHDINNAISPITLYTEALLSHEPGLSPRARKQLETIQR
                     ATDDVSQTVARMGEFYRRHESLELRPVSANKLLQEVLDMTRARWSDMAQQRGVVIDTR
                     VEESADNPVIMAEESEIREALINLVLNASDAMPAGGTLTLRTRVEDQRVILEVSDSGT
                     GMDEPTRLHCFEPFFTTKGERGSGLGLAMVYGIVQRHGGVVEISSTVGKGSTFALAFP
                     IGHRTVGVANDGSSNIRVAKATRILLIDDEPRLLQALSETLQSDGHYVQTAHDGQAGI
                     DAFLVAKISRAPFTIVFTDLGMPRMDGHQVAAAIKAIAPATTLVMLTGWGQRLAATGE
                     NPPNIDRVLGKPPNMREVRDFIASLETHPSLAA"
BASE COUNT          851 a         1558 c         1493 g          888 t
ORIGIN      
        1 agtcgacggt gccttcaccc agcgcgaccg agggcgggtc cttgccgtcg gcagcccgct
       61 tggcaacgtc tttgacgtgc aacgcgatca cgcggtcttt gtactgattg agcagcgcca
      121 cggggtcctg ctgcgccacc gccacccagc cgatgtccat ctcgatcttc acgaacttcg
      181 gatcggtcat ggcgaacagg tgatcgaacg cggtcttgcc atcgaacttt ttgaattcag
      241 cgctgtggtt gtgataaccg aaaaccatgc cggcatccgc cacctgcttg ccgaacttgt
      301 tgaagaggtc cgcgttccac ttccagtcct tgaggtcgac tgccttcatg cgctcttccc
      361 agggcagctt ttccttctcg ggcgatacgc ccggcgagga acagaccacg tacttgaggc
      421 ccagcgcctt ggcgtccgcg atgatcttgt cgccgctctt ggcgatgtcg cccgcgccgg
      481 cgtgggtgct cacaagcgag aagccgatgt ccttcatgag cttggcgaga tcggccggag
      541 ccttgccgta gtagcccgcg aattcgatgt cacggaaccc ggcggcatag acctttcgca
      601 gcgtgccttc gagatctttt tcggcctcgg acttcaccgt ccacaactgg atgcccagcg
      661 gaatcttgat gtgatgattg gcgaaagcca gtgaagaagt cagtgaaccg gctgccatac
      721 cagctcccgc ggacacgagg aataaacgtc tgctcagcat gaaagcgccc ctctgatttg
      781 ccgacgccgg agtacacggc atagggccgc cagccgtcaa gatcaggtgt cgggctgccc
      841 ggtgcactcg gcaatgacct ggcgcagctc ggcgatccgg ggcggcttgc tcaccacggc
      901 gaagacatcc gcgggagttt cacccgatgc caccagccgc tggccccagc cggtgagcat
      961 gatgatggac gtgcttggcg ctgccgcctt gatcgccgcc gccaccgcgc ggccgtcgac
     1021 gtgcggcatt cccaggtcgg ttatgaccac tggaaacggc ttgcccgctt tcaatgcatc
     1081 gcggaaagca tcgatgccca cccggccgcc gctggccgtc tgcacctggt gaccgtcgtg
     1141 caccagcacc tcacgaagtg aagtgagcag caccggatca tcgtcgatga gcagtatttt
     1201 tgtgtgcctc gggatcttct ggatgcgcag actgcccgtg gattgcgctg ccggcgacgt
     1261 cgatgggaac gtcaacctga acgtggtgcc ctcgcccggc gcgctgaaaa tgtcgatgtc
     1321 ggcgccatgg cgctgagcga tgccatacac catcgccagt ccgaggccgg aaccccgatc
     1381 ccccttggtc gtgaagaacg gttccagaca acggcggcgc gtggcttcgt ccatgccggc
     1441 tccggtgtcg ctgacctcga tgaacacccg gcgcgagacg cccttgccgg cctcgagcga
     1501 gtgccctgcg cgcagggtga gcgtgccgcc ctcgggcatc gcgtccgtcg cgttgaggac
     1561 gagattgatc aacgcctcgc gcagctcgct cgcgagggcc atgacgaccg gactttcccc
     1621 ttcgcattcg accttggtct cgatgacctt gccgcgttgc tgagccatgt cgctccaccg
     1681 tgcgcgagtc agctcgagaa cttcgcgaaa tgcctgcgtg accgacacgg gcgccagctc
     1741 catctgaccg tagcccttgc cctggcggta gaactcgccc atgcgcgcca cggtgtgcgc
     1801 gacgtcgtcg acggcacgct ggatgatttc gagttgtccc cgcgcgcgcg cgctgaagcc
     1861 ggtctcatgg gtaagcagcg cctcgacata cagcgatacg ggagagatcg cgttgttgat
     1921 gtcgtgggcg atgccgctgg ccatctggcc gagggcgcgc agcctctcct gctgcatgac
     1981 cgcgccctgg ctcacctgca ggtcgtcgta ggcctgcttg agtgagtcgt ggagttgcgc
     2041 ctggttggcg gccagcgaca cgtgatcgca caactgccgc aggaactcgc attcggcgct
     2101 gctgaacgcg tggggggcgt gccttgcgac caccagcacg ccaaacacgc cgctgcgctg
     2161 ctcgaccatc agcggcacga ccaccatcga acccaaaccg cctttcgcga ggcgctgcgg
     2221 aaacggaaag cggaccttgg agatgtccgc ctcgtacacc agcttgcccg cgacgcaacg
     2281 cgagagcccg ttctgatcga tctcgattcg ggattgctcg gtcatcgcca gctcgagcgc
     2341 cagcggtccg ctgcgctcgc cgacgcagca cacagtgagc acgcgcgtgt gatgcgtgta
     2401 gttgcacagc gcggagaagt ccgcgggcag gtgctcttcg acactgcgca ctaccacctg
     2461 gaaaatgctt tcgaggtcct ggcgctcgcc gatggcgcgc gtgatgcgtt cgagcagtgc
     2521 cagccgctcg agctgcacca gtgcgcgtcg ctcggcaacc tggcgcgtat gcgcctctgt
     2581 gcgcagatcc gtgttggccg actccagcgc agcggtacgt tcggccacgc gcgattccag
     2641 cgcctcattg ctggccttca gttcacgttc ggctgtcatg cgctgcgcgg tgtcgaatgt
     2701 ccccatctgc gtgaggctta acagcaggta gagctcgccc gcgacgcgca cgaaatgcgt
     2761 gaggatggcc atcttgtcgg cgggccccgt cgaatagagg atgagaagcg gtacgaaaaa
     2821 tgtcacggcc gcaaagaccg cgaacacttt cgcgaggcgg tcctggtggc gcaggcgcca
     2881 atagccaacg atgaccggga tccacagcag cgccgcgggt atcagcgtgg gccgcgtgat
     2941 gccgaggaac cacggtgggc tgtaacgtgg cactaccgcg aacagcacca tcatggccgc
     3001 tgaaatgaat accagcgcga cggccaacag cgccggattg gtgttgcggc ggctgagtgg
     3061 cagcgccgcg agcaggccga gcggcagtag ataggcagcg ggcgaccagg tgccgagacg
     3121 cagcaacagc gcggcgtccg gtgcttcggc gaaatcgagc gccgtgatca cgtgcagcag
     3181 ttccaggatg ctgaccgccg tgaaacacac cgcctcgagg cgcggccggc tctggcccgt
     3241 gcgccacccc aggtcccaga gcaggaacgc gagcacaccg ctcaccatga acaccgcggt
     3301 gtcgaggatg gaatgcagcg ccggaaaatc gctgctggcc gactgcatca ccgccaccat
     3361 tgccatcaac aacagcaagg cgaccgcgag catttcgcgg ttcggattca gcgagcgcct
     3421 ggcgtccatc ggccccccat gtcccagggc agccgggacc gcgtgagaat actacttgtc
     3481 caccaggcca tcgctaccgg gtaagtctca cctcaggctg caaggctcgg gtgagtttct
     3541 agtgaggcga taaaatcgcg cacctcgcgc atattcggcg gcttgccgag cacacgatct
     3601 atgttgggtg gattttcgcc cgtggcggcc aggcgctgtc cccagccggt caacataacc
     3661 agtgtcgttg cgggtgcgat cgccttgatc gcagcggcaa cctggtgacc gtccatgcgt
     3721 ggcatcccga gatcggtgaa cacgatcgtg aaaggcgcgc ggctgatctt cgccacgaga
     3781 aaagcatcga ttccggcctg accgtcgtgt gcagtctgca cgtagtgccc atcggactgg
     3841 agcgtctcgc tcagcgcctg caacaatcgc ggctcgtcat cgatgagtag aatccgagtg
     3901 gcctttgcga cacggatatt gctgctcccg tcgttcgcga ctcccacagt ccggtggccg
     3961 atgggaaaag cgagcgcgaa ggtcgaaccc ttcccaacag tgctggaaat ctcgacgacc
     4021 ccgccgtggc gctgcacgat cccgtacacc attgcgagac ccagccccga gccacgctca
     4081 cccttggtgg tgaagaacgg ctcgaagcaa tgcaaccggg tcggctcgtc catgccagta
     4141 ccggaatcgc tcacttcgag aatcactcgc tggtcttcga cgcgcgtgcg caaggtcaac
     4201 gtgccccccg cgggcatcgc atccgacgcg ttcaggacca ggttgatgag cgcttcgcgg
     4261 atttcacttt cctcggccat gatgacgggg ttgtccgccg actcttccac gcgagtatcg
     4321 atgacgacgc cccgctgctg cgccatgtcg ctccagcggg cgcgcgtcat gtccagaact
     4381 tcctgcagca gcttgttggc gcttaccggc cgcagttcca gtgactcgtg acggcgataa
     4441 aactcaccca tgcgcgccac ggtttgcgac acgtcgtcgg tggcgcgctg aatcgtttcg
     4501 agttgtttgc gcgcgcgcgg actcagcccg ggttcgtgac tgagcaacgc ttcggtgtag
     4561 agcgttatcg gcgagatcgc gttgttgatg tcatgcgcga tgccgctcgc catctgcccc
     4621 agcgcccgca atctctcgtg ttgcgtgatc ctctgctgcg acagtcgcag gtcgtcgtac
     4681 gccgattgca gatccgcgta cagcttggcg tggttagtgg cgagcgccac atgttcactg
     4741 agttggcgca ggaattcgca atcgctctcg ctgaagcctc cgacatcacg
//
LOCUS       DASQJS010000085         5702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1932648, whole genome shotgun sequence.
ACCESSION   DASQJS010000085 DASQJS010000000
VERSION     DASQJS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932648"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VEW08_03385"
     CDS             complement(<1..653)
                     /locus_tag="VEW08_03385"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYJ39806.1"
                     /translation="MNARPESALVPATPLPAEFRSALELAQAAVWDWQVAADHFQVDE
                     AWLRALGIDVHANAMPCAEWKRRIHPDDLGAFIAAADSCHHGADRFECEYRLLATGHR
                     WLWVLHRGRVVLRERDGSAQRITGLLLDIDRRKSEEMARSDSESRLATALWGARAAFW
                     QWHVPSNARTSSPLWLAMTGYSRAQWDVMPNPWFSNLHADDRERVDRQLQEHAQGER"
     gene            917..1696
                     /gene="gntF"
                     /locus_tag="VEW08_03390"
     CDS             917..1696
                     /gene="gntF"
                     /locus_tag="VEW08_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis pre-guanitoxin forming
                     N-methyltransferase GntF"
                     /protein_id="HYJ39807.1"
                     /translation="MADYSEWKARDYFQTYYSEVVLPDEQAVLAYQIDVLRAARARFG
                     RGLEYGCGPTMHRAIAASKYAFRIDMADWLADNLAEAREWLCADESAPEWKRFTRYVL
                     ACEGHSRPNNARVVQREAQTRKVVRGLYVSDARLRQPLGPTREGFYDLVITGFCIDAI
                     SSDRSVWRRCMRNVTSMLQPGGTFIIHALHQCKAYKCGDRMFPGSNLTIDDMRDAMLE
                     NGFTSSSIDAQVIPCRDNAMYGYSGILTASGRKAPARKKSH"
     gene            complement(1839..2402)
                     /locus_tag="VEW08_03395"
     CDS             complement(1839..2402)
                     /locus_tag="VEW08_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39808.1"
                     /translation="MSSHKTNKAGLVLLAATTLVVAACSSNDNDYMEPEPPAANVAPS
                     IAGIADRTSFQDSVVGPIEFSIGDRETDASMLTVSATADGTDVVPADGIALGGGGAVR
                     SITLTPLEAATGAVNVTLTVTDAQGAATMRSFRVTVNARTASIRETALAAFARGGADA
                     PTVVNGITFIQDADDPAIFEPLIGTGE"
     gene            complement(2404..3996)
                     /locus_tag="VEW08_03400"
     CDS             complement(2404..3996)
                     /locus_tag="VEW08_03400"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HYJ39809.1"
                     /translation="MQLRKHCLAAAVIASLLPVAASASSHREAPNIAGLPNVDSTDFY
                     LFNSYEPGRGDYVTVLANYIPLQQPYGGPNYFALDANALYEIHIDNDGDARENLTFQF
                     RFTNRLFDNNRGIKLNIGGQNVAVPLKNVGRVTAADNSALNFRESYQVNLVRGPRRGS
                     DGTPVTGANGATTFGKPYDYVGTNSLGTAAEYKAYADSFIHTINVPGCNMPGRVFVGQ
                     RREGFAVNLGPVFDLVNFVPVEGDSAPGAGDGGGFPGGIKQSNANNQVRNANVTTMAL
                     ELHKSCLTGSGNGVIGGWTSASLPQIRLLNPTPTFARPNAPGGAWIQVSRLGMPLVNE
                     LGIGLPDKDRFSASEPNADGQFLTYVTNPTLPALLDALFRGPVNSTLGTSYTNIAPGL
                     PRNDLVTTLLTGFANVNQLATVTPSEMTRLNTGIAATPAASQSNFGVAGGDLAGFPNG
                     RRPGDDTVDISLRVFMGALCYPLTIGGNSVDLKVCDQTKTAAQNAPVGFAPFTDGAPV
                     SAADFDTTFPYLTTPLPGSGSY"
     gene            4152..5201
                     /locus_tag="VEW08_03405"
     CDS             4152..5201
                     /locus_tag="VEW08_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HYJ39810.1"
                     /translation="MSAPFPTQKLIALARLIGGTPLLEIHCRYKGHEHRIYAKHEAYN
                     FTGSIKDRMALYILQRAYASGEIAPGDVIAEATSGNTGISFAAIGRALGHPVRIYMPD
                     WMSRERVLVIQSLGAAVIPVSAELGGFLGSIRMADDFAREAGRVFRPQQFDNAANVQA
                     HYETTGPEITAQLLTIDRTPTAFVAGVGTGGTVMGVAQHLRERFGKGIAAHPLEPANS
                     PTLRTGYKVGRHRIQGISDEFVPSIVKVGELGRILDIWDGDAIVMSQRLANELGVAVG
                     ISSGANLLGALEIAHEQGDDACVATVFCDDNKKYLSTDLCSDEECKEHYRCNDVELID
                     FRVIPACASGTPQRP"
     gene            5268..>5702
                     /locus_tag="VEW08_03410"
     CDS             5268..>5702
                     /locus_tag="VEW08_03410"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYJ39811.1"
                     /translation="MSSITRVGAIALGFAASTSFAAGVPTDDLDEVVVTAKRHQLIGH
                     VESSSQGTVTRVQLENRPVLRTGELLETVPGLVVTQHSGDGKANQFFLRGFNLDHGTD
                     LATSVDGIPVNMHTHGHGQGYTDINFVIPELLDRIEYKKGTYY"
BASE COUNT         1054 a         1840 c         1798 g         1010 t
ORIGIN      
        1 tcacgctcgc cctgggcgtg ctcctgcaat tgccgatcga cgcgctcgcg gtcgtcggca
       61 tgcaggttgc tgaaccaggg gtttggcatc acgtcccact gcgcacgcga gtaacccgtc
      121 atcgccagcc acagcggact cgaggtgcgc gcgttgctgg gtacgtgcca ttgccagaac
      181 gcggcacgcg caccccataa cgccgtcgcc agccgcgact cgctgtcgct gcgcgccatc
      241 tcttcgctct tgcggcggtc gatgtcgagc aacagtccgg tgatgcgctg cgcggatccg
      301 tcgcgctcgc gcagcacgac gcgcccgcga tgcagaaccc acaaccagcg atgtcccgtg
      361 gctaacaacc tgtattcgca ttcgaaccgg tccgcgccgt gatgacagga atccgccgcc
      421 gcgatgaacg cgccgagatc gtcgggatgg atccggcgct tccattcggc gcagggcatg
      481 gcgttcgcgt gcacgtcgat gccgagtgcg cgcagccacg cttcatcgac ctggaagtga
      541 tccgcggcga cctgccagtc ccaaaccgcc gcctgcgcaa gctcgagcgc cgaacggaat
      601 tcggccggca gcggcgtggc ggggacgagc gcggattcgg gacgtgcgtt catgaccgat
      661 tattgttatc tgccagccag acgcacactt tgatctaccg caagctttgg ggaagccggc
      721 gcccggaaac gcgaaaacgc ccacttactc atgttccgtc ctgtttttcc gcatcgacta
      781 ggtattccag taagggtcgt acggctactg caccgtgaga cacgtcagta tcctctgaca
      841 taagcctttg acaagaatct aacggaatag cgaggtcaac acagtggtag acggaagtac
      901 gacacggaat cccacggtgg ctgactactc tgagtggaaa gctcgggact acttccagac
      961 ctactacagc gaggtcgtgc tccccgatga gcaggccgtt ctcgcgtacc agatcgacgt
     1021 cctgcgcgcg gcgcgcgccc gcttcggccg cggactcgaa tacggctgcg gaccgaccat
     1081 gcaccgcgcg atcgcggctt caaagtacgc attccgcatc gacatggcgg actggctggc
     1141 ggacaacctg gccgaagcgc gggagtggct gtgcgcggat gaaagcgcgc ccgaatggaa
     1201 gcgctttact cgttacgtgc tggcctgcga ggggcattcg cgtcctaaca atgcgcgcgt
     1261 ggtgcaacgc gaggcgcaga cccgcaaggt ggtgcgcggg ctgtacgtga gcgacgcgcg
     1321 cctgcgacag ccgctgggac cgacgcgtga aggcttctat gatctggtca tcaccggctt
     1381 ctgcatcgat gcgatctcga gtgatcgctc ggtctggcgc cgctgcatgc gaaacgtcac
     1441 ctcgatgctc cagccgggcg gcactttcat catccatgcg ctgcatcagt gcaaggcgta
     1501 caagtgcggg gaccggatgt ttccgggctc gaacctcacc atcgacgaca tgcgcgacgc
     1561 gatgctggag aacggcttta cctcgtcgag catcgatgcg caggtcattc cctgccgcga
     1621 taacgcgatg tacggctact ccggcatcct cacggcttcc ggccgcaagg cgccggcgcg
     1681 caagaaatcc cactagctag gcgcgctgga tcaaccagac tacgccggcc gtgcccagcg
     1741 ggcacgaacc cgatcggaag ccgccgcgac cgccccttgg gcgtgggtcg cggcggccac
     1801 cgccagcagc gccaacagac gtgctggacc cctcactgct attcaccagt gccaatcagc
     1861 ggctcgaaga tcgccgggtc gtcggcatcc tggatgaacg tgatgccatt cacgaccgtc
     1921 ggtgcatctg ccccgcccct ggcgaaggca gccagcgcgg tctcacgtat cgaggccgtg
     1981 cgcgcattca ccgtcacccg gaacgagcgc atggtcgccg cgccctgcgc atcggtcacg
     2041 gtcagcgtga cgttcaccgc gccggtggcg gcttcgagcg gtgtcagcgt aatgctgcgc
     2101 accgcgccac cgccgccaag cgcgattcca tccgccggga ccacgtcggt tccatcggcc
     2161 gtggcgctca ccgtcagcat ggacgcatcc gtctcgcgat cgccgatgct gaattcaatc
     2221 ggacccacga cggaatcctg gaagctggtc ctgtcggcga tgccagcgat gctgggcgca
     2281 acgttcgctg ccggcggctc cggttccatg tagtcgttgt cgttgctgct gcaggccgcc
     2341 acgaccaatg tcgtggcggc gagcagcaca agacccgctt tgtttgtctt gtgcgatgac
     2401 atgttagtaa ctccccgagc ccggcagcgg cgtggtgagg tacggaaacg tggtgtcgaa
     2461 gtccgctgcc gagacgggcg cgccgtccgt gaacggcgcg aatccaacgg gcgcgttctg
     2521 ggcagccgtc ttcgtctgat cgcacacctt gagatcgacg ctgttaccgc cgatggtcag
     2581 cgggtaacac agcgcaccca tgaacacgcg cagcgagatg tccacggtgt cgtctccagg
     2641 gcggcggccg ttcggaaagc ccgcaagatc accaccggcc acaccgaagt tgctctggct
     2701 ggcggccgga gtcgctgcga tgccggtatt gaggcgcgtc atctcggagg gcgtgactgt
     2761 cgccagctga ttcacgttgg cgaagccggt gagcaacgtg gtcacgagat cgttgcgcgg
     2821 cagaccaggc gcgatattcg tgtagctggt gcccagtgtg gagttcaccg gaccacggaa
     2881 cagcgcgtcc agcaacgccg gcagcgtcgg gttcgtcacg tacgtgagga actgaccgtc
     2941 ggcgttcggc tcactggcgc tgaagcggtc cttgtcgggc agaccgatcc cgagctcgtt
     3001 cacgagcggc atgccgaggc gcgatacctg aatccatgca ccgccaggcg cattcggacg
     3061 ggcgaacgta ggagtgggat tcagaagccg gatctgcggc agcgatgcac tggtccagcc
     3121 accgatcacg ccgtttccgc tgcccgtgag acagctcttg tgcagctcga gcgccatcgt
     3181 tgtgacgttg gcgttgcgaa cctggttgtt ggcgttggat tgcttgatgc cgcctggaaa
     3241 accgccacca tcaccggcgc cgggcgcgga atcaccttcc acgggcacga agttcaccag
     3301 gtcgaacacg gggccgagat tcaccgcgaa tccttccctg cgctgaccca cgaagactcg
     3361 gccgggcatg ttgcagcccg ggacattgat cgtgtggatg aaggaatcgg cataggcctt
     3421 gtactcggcg gcggtgccca gggagttggt accgacgtag tcgtagggct tgccgaaagt
     3481 ggtggcgccg ttcgcgccgg tcaccggtgt gccatcactg ccgcggcgag ggccgcgaac
     3541 caggttcacc tggtagctct cgcggaagtt gagcgccgag ttgtcagccg cggtgactct
     3601 gccgacgttc ttgagcggca ccgccacgtt ctggccgccg atgttgagct tgataccccg
     3661 gttgttgtcg aacaggcgat tggtgaagcg gaactggaaa gtcaggttct cgcgggcgtc
     3721 accgtcgttg tcgatgtgaa tttcgtacag cgcgtttgcg tccagggcga aatagttagg
     3781 accgccgtac ggctgctgca acgggatgta attggcgagc acggtgacgt agtcaccccg
     3841 gccaggctcg tagctgttga agagatagaa atcggtcgaa tccacgttcg gtaagcccgc
     3901 gatgttcggc gcttcacgat gactggatgc cgaggctgcg actggaagca ggctcgcaat
     3961 tacggccgcc gccaggcagt gtttacggag ttgcatgatg atcccctggc tctctcgagc
     4021 gttgagttga gaaatgacgt ggggaatacg cagcgcccgc gggccggatg caccgggttc
     4081 gcgtcccgcg acaatatttc tcaaccctgc atccagcggc ctcgagtctg cgtagggtaa
     4141 gattcggccg catgtcagcg ccgtttccca cgcaaaaact cattgccctc gcgcgcctca
     4201 tcggcggcac accgttgctc gaaatccact gccgctacaa aggccacgag caccgcatct
     4261 acgccaagca cgaggcgtac aacttcacgg gcagcatcaa ggaccgcatg gcgctgtaca
     4321 tcctgcagcg cgcatacgcg agcggcgaga tcgcaccggg cgacgtgatc gccgaggcca
     4381 ccagcggcaa caccggcatt tcattcgcgg ccatcggccg cgcgctcgga cacccggtgc
     4441 gcatctacat gcccgactgg atgtcgcgcg agcgggtcct cgtcatccag agcctcggtg
     4501 ccgcggtaat tccggtgtcc gcggagctgg gcgggtttct ggggtccatt cgcatggcgg
     4561 acgacttcgc gcgtgaagcc ggccgcgtgt ttcggccgca gcaattcgac aatgccgcga
     4621 atgtgcaggc gcactatgaa accaccggcc cggaaatcac cgcccagctg ttgacgatcg
     4681 accgcactcc cacggccttc gttgccggcg tcggcacggg tggcacggtc atgggcgtgg
     4741 cgcaacattt gcgcgagcga ttcggcaaag gcatcgcggc acatccgctg gaacccgcca
     4801 actcgcccac gcttcgcacc ggttacaagg tgggccggca tcgcatccag ggcatcagcg
     4861 atgagttcgt gccctccatc gtgaaagtgg gcgagctggg ccgaatcctc gacatctggg
     4921 acggcgacgc catcgtgatg tcgcagcgcc tcgcaaacga gcttggggtc gcggtcggca
     4981 tcagctccgg cgccaacctg ctcggcgcgc tcgagatcgc gcacgaacaa ggtgacgacg
     5041 cctgcgtcgc caccgtgttc tgcgacgaca acaaaaaata cctgtcgacc gacctgtgca
     5101 gtgatgagga atgcaaggaa cactatcgct gcaatgacgt ggagctcatc gacttccgcg
     5161 tcatacccgc ctgtgcatcc gggacacccc agcgcccgta aggtgagtcg gtaacgcggc
     5221 ggaaacatgc cgcgttgtag gctacgcacc gctttcaggt atcagaaatg tcatcgatca
     5281 cacgcgtggg cgccattgct ctcggcttcg ccgcatcgac ttcgttcgcc gccggcgtac
     5341 cgaccgacga tctcgacgaa gtggtggtaa cggcaaagcg tcatcaactc atcggccacg
     5401 tggaatcttc gagccagggc accgtcactc gcgtacagct cgaaaaccgc ccggtgttgc
     5461 gcaccggcga gttgttggag accgttcccg gcctcgtcgt gacccaacac agcggcgacg
     5521 gcaaggccaa ccagtttttc ctgcgcggct tcaacctcga tcacggcacg gacctcgcca
     5581 ccagcgtcga cggcatcccc gtgaacatgc acacgcacgg gcacggccag ggctacaccg
     5641 acatcaactt cgtgattccc gagctgctcg accgcatcga gtacaagaaa ggcacgtact
     5701 ac
//
LOCUS       DASQJS010000086         9905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_48773, whole genome shotgun sequence.
ACCESSION   DASQJS010000086 DASQJS010000000
VERSION     DASQJS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48773"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VEW08_03415"
     CDS             <1..896
                     /locus_tag="VEW08_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ39812.1"
                     /translation="WRDGHLYGNLIDILNRAAPLALVAAGMTLVIAARGLDISVGAVV
                     AIAAATGALCVSATGSLGLAVVAALAVALLCGLWNGLLVVGVGMQPIIATLILMVAGR
                     GVAQLITGGQIITIYYAPYDFIGNGFLLGLPFAAVLAVVVIVLLHALLTTTPLGLFIR
                     AIGINPLAARIAGVRARPITLCLYGFCGLMAGLAGLIISSNVKSADANNAGTLLELDA
                     ILAVTLGGTALTGGRFSLAGTAIGALIIQTLTSTIYSLGVPPEVNLVFKAALIFVVML
                     LQSAEFRASIRGLATRPVDPA"
     gene            893..1900
                     /gene="yjfF"
                     /locus_tag="VEW08_03420"
     CDS             893..1900
                     /gene="yjfF"
                     /locus_tag="VEW08_03420"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF008630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HYJ39813.1"
                     /translation="MTTGGITFALPGFDRRMLPLTATLLLFFAMVAFGAAAYDGFFAP
                     QVFLNLAIDNAFLLIAAVGMTFVILTGGIDLSVGSVLAFTTMAAAALIEHAQLSPWLV
                     MPLLLAAGAAFGALQGFLIHRYRLQPFIVTLAGMFVARGLCYLIDTESIPIRDPVFVA
                     LAEMRIPLGGSSLSSGAAVALVTVAAALWLAHATRFGRTVYAIGGNARSAALMGLPVG
                     VTLMRVYAMSGFCAALAGLTMTVYMLSGYSLHAVGLELDAIAAVVIGGTLLTGGVGYV
                     AGTVVGVLILGIIQTLVIFNGTLSSWWTRIVVGILLFLFCVAQRLIEGSTQKSNNKER
                     R"
     gene            1897..2907
                     /locus_tag="VEW08_03425"
     CDS             1897..2907
                     /locus_tag="VEW08_03425"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HYJ39814.1"
                     /translation="MIRPFGELEDGRSIEAISLGHEDGLQVEVLTYGAILRRLSYPVR
                     GVRRDLILNFDRLEHYERDRAYVGSLVGRFGNRIAGSRFDLDGRTHEVTANEGVNHLH
                     GGASGFGKRVWRVLDITHSEAPRVILGYLSPDGEEGYPGNVEATAELVVKRDALSIIL
                     SARSDAATPINLTYHPYFNLQADRNLPATEQRLRIPADHYLPVVPGLIPTGQLASVEG
                     TTFDFRACRRLRPPPVTGHPQLALAGGYDHCWVLAANADCACELSSPDGDITLTMSGG
                     PGLQFYNGQFLSRAHPLIGSGVILEPQGFPNAPNEPHFPPAILRRGEVYRAGIEYRLE
                     AS"
     gene            3118..4761
                     /locus_tag="VEW08_03430"
     CDS             3118..4761
                     /locus_tag="VEW08_03430"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HYJ39815.1"
                     /translation="MNNYSSRRRFLSQVGLAGAGIAASSVLALPSYSQGSGRTARATL
                     GLVSPRADFNPRLFGAFLEHLGRSIYTGVYDPGNSLSDERGFRKDVIKEVADLHVPII
                     RYPGGNMVSGYNWWDGVGPKKDRPTVLERAWNSIETNQFGTNDFMEWAKAVGTEPLLG
                     FNLGTGTSEMAVAYIEYCNYAKGTKWSDLRRSHGYEQPHKVKYWCLGNEMDGPWQMGR
                     LTAREYGRKARDIAQQARVIDPDVQLIACGSSNTILPTYLTWDREVLEECYDQVDGIS
                     LHNYFGNEPELAGGKSERFLAQNLDMERQILEITAVADYVQGLYRSPKKLWLSFDEWN
                     VWYRARTPDHLDGKGQFAPKLLEEVYNLEDAMLTGGFLNTLMRQSDRVRVACLAQIVN
                     VIAPLVTNSKGTLRQSIYYPYQMVLKYARGRMLDIQVESETYPIRAAGLRPDFARDEN
                     VPFLDVVATYDAKEKRIAVFALNRDISNERELALTFDDITPSKVLACETVTGTDLKAF
                     NTFESPNKVVTTKLDTAKAGRKMSVKLPARSYTVVHMAV"
     gene            4766..6682
                     /locus_tag="VEW08_03435"
     CDS             4766..6682
                     /locus_tag="VEW08_03435"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HYJ39816.1"
                     /translation="MTDIPHSRREALKLMVGAGAGAASFACAPCAQANVVSVDVSEKG
                     RYFPLAQVRLGPGPFLEAQKLDAAYLLQLEPDRMLHNFRVNAGLKPKAPVYGGWESVE
                     PWIEIRCHGHTLGHYLTAAACMFESTGDARFAERVDYIVAELAEYQARTGGWLTAFPD
                     GVAPLVDSLAGRPFPGVPWYTTHKVMAGLRDAYLHRGSKRALDVLVRFTQWIHDASID
                     VPAGQMQKMLDREHGGMNEVLADVGKIAGDEGFFKLAQRFSHRALLDPLVERRDTLDG
                     LHANTQIPKVVGFARIAEHTGVPRYADAAEFFWSTVVGTRSYATGGHGDVEHFFPRNE
                     FEKRLGSGKTMETCCTHNMMRLTRALYARDPREEYWDYFEKALFNGILASQDPATGMM
                     TYFQSTRPGYVRLYHTPFDSFWCCTGSGLENHARYGEAIYARNEDDLIVNLFIASTLD
                     WRERGIKLTQETDFPDAEFTRLTFTLDRPQTFGLSIRKPAWCDGMKVTMNGKTVKGSS
                     LRRRFRSGDVVEVRLPMKLRVETLPGETAVGALVYGPIVLAARLGTSGLSPGSQLIVN
                     ERQSGEMLNENMDIPAWNRPLAELVANTSRTDPSSLRFTTRGFAGGVTVEMIPWFRLT
                     HERYNLYWKGATAA"
     gene            6881..7246
                     /locus_tag="VEW08_03440"
     CDS             6881..7246
                     /locus_tag="VEW08_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ39817.1"
                     /translation="MAIHKVLIVDDARPDLMNLEKIVSGAGYITLTATNGQQAVEMSK
                     AQMPDVILMDVNMDQMDGFAATRAIGGDAATKTIPVVLVTSKNQKADRVWAQMLGVKG
                     YVTKPYTPDQILSAISACA"
     gene            7266..7778
                     /locus_tag="VEW08_03445"
     CDS             7266..7778
                     /locus_tag="VEW08_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HYJ39818.1"
                     /translation="MNATSPLPLGLPPLPAELAALAAGPIEENPNDIVRQAFRIGHMR
                     LLAPFATASELVEMPNVFPLPRMPANLLGLVNLHGRIVPLFDLAPLFETQHLPREKRM
                     LLVIGHGEAAAGIVIDGLPRRMVFQPENEILTPALSPAATTAVVATYLQGSDAWFEFN
                     YAHLLDQLVS"
     gene            7783..8364
                     /locus_tag="VEW08_03450"
     CDS             7783..8364
                     /locus_tag="VEW08_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39819.1"
                     /translation="MAIRNVFGAQSIRQKLLLGTLFLAIVPVAVTSIIVGRESLNSGR
                     AALEAQAREALVAQRASKAAQITDYFDALSNQVQVLAASPDVVNSMRELPAAYDNSVL
                     NVADLPAERARLAKYYTAEFMAEFQRRNTGKMVNMADTAAGLPDLAMNLQYQYIAANP
                     HPLGNKNNLDRANDGSRYSELHGALHPYLRTAL"
     gene            8425..>9905
                     /locus_tag="VEW08_03455"
     CDS             8425..>9905
                     /locus_tag="VEW08_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYJ39820.1"
                     /translation="MFKELDFATSLVSGPYAKTKLGDAFRQSWSLDKPGMIALSEFGE
                     YLPSYNDQAAFLGTPIFDGAKKIGVLVVQVPIDKINSVMTHEGKWKERGLGNTGETYL
                     VSAADGTPRSVARLAVEDINAYAQSVTEAGYAQGLANAVQAKGTSIGLVPIKTQATQD
                     VFEKGASGFGVYPNYAKRPVLGAYAPLSVLGLRWAIIAEENATEAFAPVTAVREKVVV
                     WSTGIAVALLGLGLLIAFSLLRSVTRPITKMQQTVAAIADGDYAARSQLGGDDEFGQL
                     SRAFDDLLDDRVARLADAERENEDLNNSVVQLLQAVAQLSNKDLTAKAPVTANVIGTV
                     SDSINLLSYETGRVLSEVTSIAAQVKLSTERVKRQGESVTATAAAERSDVERAGENLA
                     NASKVMTQVAELAAASNRAAEQATSSTVTALASVNETVKGMEGIRESIAESEKRIKRL
                     GERSQEITGIVNLINTIAERTHVLALNASMQAAAAGDAGRGFAV"
BASE COUNT         1768 a         3358 c         3034 g         1745 t
ORIGIN      
        1 cctggcgcga cggccacctg tacggcaacc tcatcgatat cctgaaccgc gcagcgccgc
       61 tggcgctggt cgccgccggc atgacgctgg tcatcgcggc gcgcggcctc gacatctcgg
      121 tgggcgcagt cgtcgcgatc gccgccgcca cgggcgcgct ttgcgtgagc gccaccggca
      181 gcctggggct cgccgtcgtc gcggcgctgg cggtggcgct gctgtgcgga ctgtggaatg
      241 gcctgctggt cgtgggcgtc ggcatgcagc ccatcatcgc cacgctgatc ctgatggtgg
      301 ccggccgcgg cgtcgcccag ctcatcacag gcggccagat catcaccatc tactacgcac
      361 cttatgactt catcggcaac ggcttcctgc tcggcctgcc gttcgccgcg gttctcgccg
      421 tcgtggtcat cgtgctgctg cacgcgctgc tgaccaccac gcccctgggt ctgttcattc
      481 gcgccatcgg catcaacccg ctggccgcac gcatcgccgg cgtgcgtgcg cgacccatca
      541 ccctgtgcct ctacggcttc tgcggcctca tggccgggct cgcgggcctc atcatcagtt
      601 ccaacgtcaa gagcgcggac gccaacaatg ccggcacgct gctcgagctc gacgcgattc
      661 ttgccgtcac gctcggcgga accgcgctca ccggcggccg cttcagcctc gcgggcaccg
      721 cgatcggcgc gctgatcatc cagacactca cctctactat ctactcactc ggcgtgccgc
      781 ccgaagtgaa cctggtgttc aaggcggcgc tgatcttcgt ggtgatgctc ctgcagtcgg
      841 ccgagttccg cgcgagtatc cgtgggctcg ccactcgccc ggtggacccg gcatgaccac
      901 gggcggcatc actttcgccc tgcccggctt cgacagaagg atgttgccgc tgacggcgac
      961 actgttgctg ttcttcgcca tggtggcgtt cggcgccgcg gcgtacgacg gattcttcgc
     1021 accccaggtg ttcctcaatc tcgcgatcga caatgcgttt ctgctgatcg ccgccgtcgg
     1081 gatgacgttc gtgattctca ccggtggcat cgacctctcg gtgggctccg tgctggcgtt
     1141 caccaccatg gccgcggcgg cgctgatcga gcacgcccag ttgagcccgt ggctggtgat
     1201 gccattgctg ctggcggcag gcgccgcgtt cggcgccctg cagggatttc tcatccaccg
     1261 ataccggctg cagccgttca tcgtcacgct cgccggcatg ttcgtggctc gcgggctctg
     1321 ttatctcatc gacaccgagt cgatcccgat tcgcgacccg gtgttcgtgg ctctggccga
     1381 gatgcgcatt ccgctgggtg gcagttccct gtcgagcggc gcggctgtag cgctcgtcac
     1441 cgtggcggcg gccttgtggc tggcgcacgc aacgcgcttc ggacgcaccg tgtacgcaat
     1501 cggcggcaac gcgcgctcgg cggcactcat gggtctgccc gtgggcgtga cgctcatgcg
     1561 cgtgtacgcg atgagcggct tctgcgccgc gctcgcgggg ctcacgatga ccgtctacat
     1621 gctgtcgggc tacagcctgc atgccgtcgg cctcgagctc gatgccattg cggcggtggt
     1681 catcggtggc accttgctca ccggtggtgt gggctatgtc gccggcaccg tagtcggggt
     1741 gctcatcctc ggcatcatcc agacgctggt gatcttcaac ggcacgttga gctcctggtg
     1801 gacgcgcatc gtcgtcggga tcctgctgtt tcttttctgt gtggcgcagc ggctcatcga
     1861 gggcagcacc cagaagtcca acaacaagga gcggcgatga tacgtccgtt cggagagctc
     1921 gaagacggcc gcagcatcga ggcgatctcg ctcgggcacg aggacgggct gcaggtcgaa
     1981 gtgcttacct acggcgcaat cctgcggcgc ctgagttatc ccgtgcgcgg cgtgcgccgc
     2041 gatctcattc tcaacttcga tcgcctcgag cactacgaaa gagaccgggc gtacgtcggt
     2101 tcgctggtgg gccgctttgg caatcgcatt gccggctcgc gcttcgatct cgacggcagg
     2161 acccacgaag tcaccgccaa cgagggcgtc aatcatctgc atggcggcgc atcgggattc
     2221 ggcaagcgcg tctggcgcgt gctggacatc acccatagtg aagcgccacg cgtgatcctg
     2281 ggttacctgt cgcccgacgg cgaagaagga tacccgggca atgtcgaagc cacggccgag
     2341 ctggtcgtga aacgcgacgc gctgtccatc atcctgagcg cgcgcagtga cgccgccacg
     2401 cccatcaacc tcacctatca cccgtatttc aacctgcagg ccgatcgaaa cctgcccgcc
     2461 accgagcagc ggctgcgcat tcccgcggac cactatctac ccgtcgtccc cggcctcatt
     2521 cccaccgggc aactcgcctc tgtcgaagga accacattcg actttcgcgc gtgccgccga
     2581 ttgcgcccgc cacccgtgac cggacatccg cagctcgcgc tcgccggcgg ctacgaccac
     2641 tgctgggtgc tggcggcgaa cgcggattgc gcctgtgagc tttcctcccc ggacggagac
     2701 atcacgttga cgatgtcggg cggcccgggc ctgcagtttt acaacgggca attcctttcg
     2761 cgcgctcatc cgctgattgg cagtggcgtg atactcgaac cgcagggctt tccgaatgcg
     2821 cccaacgaac cacactttcc accggcaatc ctgcgtcgcg gagaggtgta tcgcgccggc
     2881 atcgaatacc ggctggaggc gtcatgagtg acgttttcag gagggcccgg cgccgccgcg
     2941 cagctattgc ggaagacggc gccggtttcg ttccacggct ttgcatgcag gatccggtca
     3001 aacgtgcggg ttcttccggt gcatgcgaag acgtagcgcg atcgtattca gcacttcgcg
     3061 cagcgtccat gagtggtaac ggtacctcac aaaaatattt tctacggagc aaagccaatg
     3121 aacaactact cgagccgacg tcgttttctt tcccaggtgg gcctagctgg cgcgggaatc
     3181 gccgcctcct ccgtgttagc gctacctagc tactcacagg gcagcggccg caccgcccgc
     3241 gcgacgcttg gcctcgtgtc accgcgcgcc gatttcaacc cgcgactgtt cggcgcgttc
     3301 ctcgaacatc tcgggcgctc gatctacacc ggcgtctacg accctggcaa ttccctctcc
     3361 gacgagcgcg gcttccgcaa agacgtcatc aaggaagtgg ccgacctgca cgtgccgatc
     3421 atccgttacc cgggcggcaa catggtctcg ggctacaact ggtgggacgg ggtgggcccg
     3481 aaaaaggacc gacccaccgt gctcgaacgc gcgtggaact cgatcgagac caaccagttc
     3541 ggcaccaatg acttcatgga gtgggccaag gcggtgggca ccgagccctt gttaggattc
     3601 aacctgggca cgggcacctc ggaaatggcg gtcgcctaca tcgagtactg caattacgcg
     3661 aagggcacca agtggagcga cctgcgccgt tcgcatggct acgagcagcc ccacaaggtg
     3721 aagtactggt gcctcggcaa cgagatggat ggcccctggc agatgggccg tctcacggcg
     3781 cgcgagtacg gccgcaaggc gcgcgacatc gcccagcagg cgcgcgtcat cgacccggat
     3841 gtgcagctca tcgcctgcgg ctccagcaac accatcctgc ccacgtacct gacctgggat
     3901 cgcgaggtgc tggaggagtg ctacgaccag gtcgacggca tctcgctgca caactacttc
     3961 ggcaacgaac ccgagctcgc gggtggcaag tccgagaggt tcctggcgca gaacctcgac
     4021 atggagcggc agatcctgga aatcaccgcg gtcgccgatt acgtgcaagg cctgtatcgc
     4081 tcgcccaaga aattgtggct ctcgttcgac gagtggaacg tctggtaccg cgcacggacg
     4141 cccgatcatc tcgatggcaa gggccagttc gcgcccaagc tgctcgagga ggtgtacaac
     4201 ctggaagacg ccatgctcac gggcggcttc ctcaatacgc tcatgcgcca gtccgaccgg
     4261 gtacgcgtcg cctgcctcgc gcagatcgtc aacgtcatcg cgccgctggt caccaacagc
     4321 aaaggcacgc tgcgccagtc catctactac ccgtaccaga tggtgctcaa gtacgcgcgc
     4381 ggccgcatgc tcgacatcca ggtggagagc gaaacctacc cgatccgtgc cgcgggcctt
     4441 cgcccggact tcgcgcgcga cgagaacgtg ccattcctcg acgtcgtcgc cacctacgac
     4501 gcgaaggaaa agcgcatcgc ggtgttcgcg ctcaaccgcg acatttcgaa cgagcgcgag
     4561 ctggcgctca cgttcgacga catcacgccg tcgaaggtgc tcgcttgtga aacggtgaca
     4621 ggcaccgatt tgaaggcgtt caacaccttc gagagtccca acaaggtcgt cacgacgaag
     4681 ctcgacaccg cgaaagcggg ccggaagatg tcggtgaagc tgccggctcg ttcgtacacc
     4741 gtcgtgcaca tggcggtgtg accccatgac cgatataccg cactcgcgcc gggaagccct
     4801 caagctgatg gtgggggctg gcgcgggcgc ggcctcgttc gcatgtgcgc cgtgcgcgca
     4861 ggcgaacgtg gtatctgtcg acgtttcgga aaagggccgc tacttccctc tcgcgcaggt
     4921 acgcctgggg cctggccctt tcctcgaggc gcagaaactc gatgctgcct atctattgca
     4981 gctcgagccg gaccgcatgc tgcacaactt ccgcgtgaat gcgggcctca agcccaaagc
     5041 cccggtgtac ggcggctggg aatcggtcga gccctggatc gaaatccgtt gccacggcca
     5101 cactctcggc cactacctga cggccgccgc ctgcatgttc gaatccacgg gcgatgcgcg
     5161 attcgccgaa cgcgtggatt acatcgtggc cgagctcgcg gagtatcagg ccaggaccgg
     5221 aggctggctc acggcattcc cggacggcgt cgcgccgctc gtggacagcc tcgcgggcag
     5281 gccttttccg ggcgtgccct ggtacaccac gcacaaagtg atggcgggcc tacgcgatgc
     5341 gtatctgcac cgcggcagca agcgcgctct cgacgtactc gtgcgattca cacaatggat
     5401 ccatgacgcg agcatcgacg tgcccgccgg gcaaatgcag aagatgctcg accgcgaaca
     5461 cggcggcatg aacgaggtgc tcgcggacgt cggtaagatt gccggtgacg aaggcttctt
     5521 caaactcgcg cagcgctttt cgcaccgcgc actgctagat cctctcgtcg aacgccgcga
     5581 cacgctcgat ggactgcacg cgaatacaca gatccccaaa gtcgtgggct tcgcacgcat
     5641 cgccgagcac accggggtgc cgcgatacgc cgacgccgcc gagttcttct ggagcaccgt
     5701 ggtcggaacg cgcagctacg ccaccggcgg ccacggcgac gtggagcatt ttttcccgcg
     5761 caacgaattc gaaaagcgcc tgggctccgg caagaccatg gagacctgtt gcacgcacaa
     5821 catgatgcgg ctcacgcgtg ccttgtacgc tcgagatccc cgcgaggaat actgggacta
     5881 cttcgagaag gcactcttca atggcattct cgcctcgcag gatcctgcca caggaatgat
     5941 gacttacttt cagtccacgc gaccggggta cgtgcgcctg tatcacacgc cgtttgactc
     6001 cttctggtgc tgcacggggt cggggctcga aaaccacgcg cgttacggcg aggcgatcta
     6061 cgcacgcaac gaagacgacc tgatcgtcaa cctgttcatc gcctccacac tcgactggcg
     6121 cgagcgcgga atcaaactga cgcaggagac tgactttccc gacgcggagt tcacgcgact
     6181 cacgttcacg ctcgatcgcc cgcagacgtt tggcctttcc atccgcaagc cggcctggtg
     6241 cgacggcatg aaagtgacga tgaacggcaa gaccgtgaag ggctcgagcc tgcggcgccg
     6301 tttccggtcg ggtgatgtcg tcgaggtgcg cttgccgatg aagctgcgcg tcgagacgct
     6361 gccgggcgag actgccgtgg gcgcgctcgt ttacgggcct attgtcctcg cagcgcggct
     6421 cgggacctcg ggcctttcgc ctggctcaca actcatagtc aatgagcggc aatccggcga
     6481 gatgctcaac gagaacatgg atatccccgc ctggaatcgc ccgctcgccg agctggtggc
     6541 caacacttcg cgcaccgacc cgtcgtcgct caggttcacc acgcgcggct ttgccggcgg
     6601 cgtcaccgtg gagatgattc cctggttccg gctcacccat gagcgctaca acctctactg
     6661 gaaaggcgcc accgccgcct gagggcggct tgtgaactgg taacggtcgc aagccggcga
     6721 aacccggaga aatcgcgaac tggagccttt cctagggaca ccccgtgtga cccgcgtcac
     6781 cgtggttttg tcacataccg gccaagattc ccctcgtgcc aatgggagaa accgtccgaa
     6841 gcggtcggcg gggcctcatt gcgcgtgagg gagagctgtt ttggctattc ataaagtatt
     6901 gatcgtcgat gacgcgcgtc ctgatctcat gaatctcgag aagatcgtga gcggagcggg
     6961 ctacatcacc ctcacggcca ccaatggcca acaggccgtc gaaatgtcca aggcgcagat
     7021 gccggacgtc attctcatgg acgtcaacat ggaccagatg gacggctttg ccgccacgcg
     7081 cgccatcggt ggagacgccg cgaccaagac catccccgtg gtgctggtca ccagcaagaa
     7141 ccagaaggcc gaccgcgtgt gggcccagat gctcggtgtg aagggctacg tcaccaagcc
     7201 ctacaccccg gaccagattc tcagcgccat ctcggcctgc gcttaaggcg accgcacccg
     7261 aatacatgaa cgccacctct cctctgcctc tcggccttcc tccgcttccc gcggagctgg
     7321 cggcattggc tgcgggcccg atcgaggaaa acccgaacga catcgttcgc caggcgttcc
     7381 gcattggcca catgcgcctg ctagcgccgt tcgcgaccgc gagcgaactc gtcgagatgc
     7441 ccaacgtctt tccgttgccg cgtatgcccg ccaacctgct ggggctggtg aacctgcacg
     7501 gccgcatcgt gcccctgttc gatctggcac cgctgttcga gacacaacac ctgccgcgcg
     7561 agaaacgcat gttgctcgtg atcggtcacg gcgaggccgc cgccggcatc gtcatcgacg
     7621 ggctcccgcg ccggatggtc ttccagccgg aaaacgaaat cctcacaccg gcgttgtccc
     7681 ccgccgccac caccgccgtg gtcgccacct acctgcaggg atccgacgcc tggttcgaat
     7741 tcaattacgc gcacctactc gatcaactcg tgagctgaca atatggccat tcgtaacgtc
     7801 ttcggcgccc agtcaatccg acagaaactt ctgctcggta cgctcttcct cgcgatcgtc
     7861 cctgtcgccg tcacgtctat tatcgttggt cgcgaatccc tgaactccgg ccgcgccgca
     7921 ctcgaagccc aggcccgcga agcgctggtc gcccagcgcg catcgaaggc cgcgcagatc
     7981 accgactatt tcgatgcgct gtcgaaccag gtgcaggtgc tcgccgcctc tccggacgtc
     8041 gtgaactcca tgcgcgagct gccggccgcg tatgacaact cggtactgaa cgttgccgac
     8101 ctgcccgccg agcgcgcgcg cctcgccaag tactacaccg ccgaattcat ggccgagttc
     8161 cagcgccgca acaccggcaa gatggtcaat atggcggaca ccgcggccgg cctgcccgac
     8221 ctcgcgatga acctgcagta ccagtacatc gccgcgaacc cgcatccgct cggcaacaag
     8281 aacaacctcg atcgcgccaa cgacggctcg cgctacagcg agctgcacgg tgcgctccat
     8341 ccgtacctgc gcacggcgct ctaacagttc ggcctgtacg acatcttcct gatcgagccg
     8401 cgcaacggca ccatcgttta cacggtgttc aaggagctcg atttcgcgac ctcgctggtc
     8461 agcggcccgt acgccaagac caagctcggc gacgcattcc gccagagctg gtcgctcgac
     8521 aagcccggca tgattgcgct gtcggagttc ggtgaatacc tgccttcgta caacgaccag
     8581 gccgccttcc tcggcacccc gattttcgac ggcgccaaga agatcggcgt gctcgtcgtg
     8641 caggtgccca tcgacaagat caactcggtg atgacgcacg aaggcaagtg gaaagaacgc
     8701 ggcctcggca acaccggcga aacttacctg gtgtcggcgg ccgacggcac gccgcgatcc
     8761 gtcgcgcgcc tcgcggtgga agacatcaac gcctacgcgc agagcgtgac cgaagccggt
     8821 tacgcccagg gcctggcgaa tgccgtgcag gccaagggca cgagcatcgg tctcgtcccg
     8881 atcaagacgc aggccacgca ggacgtattc gaaaaaggtg cttccggctt cggcgtgtat
     8941 cctaactacg ccaagcgccc ggtgctgggc gcgtacgcac cgctgtcggt gctggggctg
     9001 cgctgggcga tcatcgccga ggaaaacgcc acggaagcgt tcgcgcccgt gaccgcggtg
     9061 cgcgagaaag tggtggtgtg gtcgacgggt atcgcggtcg cgctgctcgg actcggcctg
     9121 ctcatcgcgt tctcgctgtt gcgttcggtc acgcgtccta tcaccaagat gcagcagacc
     9181 gtggccgcga tcgccgacgg cgactacgcc gcgcgttcgc agctcggtgg cgacgacgaa
     9241 ttcggccagc tctcccgcgc cttcgacgac ctgctcgacg accgcgtggc gcgcctcgcc
     9301 gacgccgagc gcgaaaacga agacctcaac aactcggtcg tgcagctcct gcaggccgtg
     9361 gcgcagctct ccaacaagga cctgaccgcc aaggccccgg tcacggcgaa cgtgatcggc
     9421 accgtgtccg actccatcaa cctgctgtcg tacgaaaccg gccgcgtgct gtcggaagtg
     9481 accagcatcg cggcgcaggt gaagctctcc accgagcgcg tgaagcgcca gggtgagtcg
     9541 gtgaccgcaa cggcagccgc ggaacgttcg gacgtcgaac gcgcgggcga gaacctcgca
     9601 aacgcctcga aggtcatgac gcaggtggcc gaacttgctg ccgcttccaa ccgtgccgca
     9661 gagcaggcca ccagctcgac ggtgaccgcg ctcgcgtcgg tgaacgaaac cgtcaagggc
     9721 atggaaggca ttcgcgaatc catcgccgaa tccgaaaagc gcatcaagcg cctgggcgaa
     9781 cgttcgcagg aaattaccgg catcgtgaac ctcatcaaca cgatcgccga acgcacgcac
     9841 gtgctggcgt tgaacgcctc catgcaggcc gccgccgccg gcgacgcggg ccgcggcttc
     9901 gcggt
//
LOCUS       DASQJS010000087         6347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4128440, whole genome shotgun sequence.
ACCESSION   DASQJS010000087 DASQJS010000000
VERSION     DASQJS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4128440"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VEW08_03460"
     CDS             <1..367
                     /locus_tag="VEW08_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39821.1"
                     /translation="ASGRNWVFFGGRSLYHDFLYQLEWQQALKRKSVHRVDVAFSRDQ
                     AEKIYVQHRIRQAGKELFAWLSNGASFYVCGDASAMAPDVHAALLDVVREQGNMDADD
                     AQAWVADLTADGRYLRDVY"
     gene            357..2084
                     /gene="cysI"
                     /locus_tag="VEW08_03465"
     CDS             357..2084
                     /gene="cysI"
                     /locus_tag="VEW08_03465"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF010029.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HYJ39822.1"
                     /translation="MSTEVLDRPAVAPAKTDPPSPVEVIKKASRGLRGTLAESLADPL
                     TGAIRESDTQLIKFHGSYQQDDRDIRAEREHQKLEPAYSFMIRTRTPGGVCTPKQWLA
                     LSQLARRYGSGTLRLTTRQAFQLHGVIKKDLKATMQGMQAALIDSIAACGDVNRNVMA
                     SANPVESRLHEEVHQWAMKVSEHLLPKTRAYHEIWLNGEKLAGPAEEEPILGQLYLPR
                     KFKTGIVVPPHNDVDVFSQDLGFIAILETEGLEEGKLLGFNLSVGGGLGATHGEPQTF
                     PRLADVIGFLKPEQMLKVAETVVMIQRDFGDRTDRKHARLKYTIADRGIDWFKAELGR
                     RLGYELEPARPYNFTTQGDRFGWLRGHDGRWHLTLRIEAGRVGDTPGQPFLTGLEKIA
                     AIHQGDFRLTANQNLIIANVPASECMNIDKLVADHGLGKALFTTPLRRDALACVALPT
                     CALAMAEAERYLPNFTDRVEALLFKHGLREIPLTLRITGCPNGCARPYLAEIALIGRA
                     PGRYTLRLGADAVGSRLNVVYRDNIDENAIVAVLDELIARYAVERHTHERFGDFLWRA
                     NVLRQETRA"
     gene            2081..2875
                     /locus_tag="VEW08_03470"
     CDS             2081..2875
                     /locus_tag="VEW08_03470"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HYJ39823.1"
                     /translation="MIESVAREAPKFSTAPVSLVGAPRLTLSAKEFAGDPDAANRINT
                     WLESVDAATRVRWALENLPGPFALSSSFGAQAAVSLHLVAQADPDIPVLLIDTGYLFP
                     ETYQFIKQLEQQLSLNIQTFSSPQSVDEFEAQYGKLWEQGREGLDKYLELRKVEPMRR
                     GLATLGTATWFAGLRRSQAASRAALSPLEVRDGRFKVHPIFDWSDRDVYLYLKAQGLP
                     YHPLWDKGYVSIGDWHSTKALHEVESADELRFSGLKRECGIHGLEG"
     gene            3009..4529
                     /locus_tag="VEW08_03475"
     CDS             3009..4529
                     /locus_tag="VEW08_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39824.1"
                     /translation="MQDMTLDKPIVLGARASVLAQVQARLVVDALLARHPGLKIEYSF
                     LTSPGDRELDTPLAELLRRGGFTGDLGVALRANAIDIAVHLWKDLPFAGNPLTHIAAT
                     LPRADTRDLLLVKRSWLSRSQGELRVLSSSARRRTNLSGFLQWALPIQPPRVTFVLAR
                     GDSEARLAQLMDGEAAALVVAKANIDRLLESTAPEFAGLRERVRAALDACEVMVLPLA
                     LNPTAPGQGALAIEIRRDRADLAELLAPINDEATFGRVIAERARLSPTGDEDHPLGVS
                     IVPIHCGEVEFTRGQKGGQWIQSAELRRHDGPLPPASSQHAIWTGDEGSADRYRRVAL
                     QVRHDQFADARVGLFVARSEAWPDGFSPRPGQVVWSAGIDTWRRLAARGIWVHGSDES
                     LGEGGSGALRSLFPRVQRWVKFSHEQGYDSPFSERIPTYRLERCAPLADLTGRTHFFW
                     RSGAQFYDYLQANPEIRGAWHGCGPGNTFSMIRAAIGEERVRAFLSADVFRQEVEQ"
     gene            4526..5485
                     /gene="hemB"
                     /locus_tag="VEW08_03480"
     CDS             4526..5485
                     /gene="hemB"
                     /locus_tag="VEW08_03480"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HYJ39825.1"
                     /translation="MKRLQRLRATAALRDMVSEIGFGQSQLIQPLFLSEAAVKEEPIA
                     GLQGTSRHTLSSLLTQFDKDLGAGVRQFLLFPVPAHKSNRSFQAEFVGSALGQMRQRA
                     GDDATIWVDTCLCSFTESGHCQIHDARGKQDLAATHSALAVLGLTYVKAGANGIAPSD
                     MNDGRVAHLRATLDGSGFDLAPIMSYSTKFASNYYGPFRDAADSAPQFGDRRSYQLDV
                     RNRSDALASSRRCAEEGADLLMVKPGQPSADLIRPIHEQTGLPVGAYQVSGEYTSLAL
                     LARENYVEFDAALLESWYNLRRAGASYIITYGARLAQGLGLPR"
     gene            5485..>6347
                     /locus_tag="VEW08_03485"
     CDS             5485..>6347
                     /locus_tag="VEW08_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ39826.1"
                     /translation="MRYFPLFLDIKDKPVLLVGGGKVAARKFSLLVDAGARITVVAPR
                     LGDELTAALARGAVTHHAREFTQTDIGGAWLVVAATDDRAVNAAVSAAANAARIPCNV
                     VDDRELSSFIMPAIIDRSPVQIAISTGGTSPVLARLIRERLETLLDSSLGPLAAFADR
                     WRVAVKARFADIGARRRFLSWMLTGPVAAAVRAERHGQAEELTRKALEKADGIPAGHV
                     VLVGAGPGNSGLMTLQGLRALQEADVIVHDRLVSAEVMDLARRDAARFDVGKFVGGGG
                     ATQEEINKLLV"
BASE COUNT         1084 a         2171 c         1996 g         1096 t
ORIGIN      
        1 cgcgtccggg cgcaactggg tgtttttcgg cggccgctcg ctgtatcacg acttcctcta
       61 ccagctcgag tggcagcagg ctctcaagcg caagtccgtg catcgcgtgg acgtggcgtt
      121 ctcgcgcgac caggccgaaa agatttacgt gcagcatcgc atccggcagg ccggcaaaga
      181 gctctttgcc tggctgtcga acggtgcctc cttttatgtg tgcggtgacg cttccgccat
      241 ggcgcccgat gtgcacgccg cgttgttaga cgtggtgcgc gagcagggca acatggatgc
      301 ggacgacgcc caggcctggg tggccgacct caccgctgac ggcaggtatc tacgcgatgt
      361 ctactgaagt actcgatcgg ccggccgtgg cgccggccaa gactgacccg ccttcgcccg
      421 ttgaagtcat caagaaggcg agccgcggcc tgcgtggcac gctggccgaa agccttgccg
      481 atccgctgac cggcgcgatc cgcgagtccg atacgcagct catcaagttt cacggcagct
      541 accagcagga cgatcgcgac atccgcgccg agcgtgagca ccagaagctc gagcctgcgt
      601 acagcttcat gatccgtact cgcacgcccg gcggcgtgtg cacgcccaag cagtggctgg
      661 cgctgagcca gctcgcgcgc cgttacggca gcggaacgct gcgcctgact acccgccagg
      721 ctttccagct tcatggcgtg atcaagaaag acctgaaagc caccatgcag ggcatgcagg
      781 ccgcgctcat cgattccatt gccgcctgcg gcgacgtgaa tcgcaacgtg atggcgagcg
      841 ccaacccggt ggaaagccgg ctgcatgaag aagtgcacca gtgggcgatg aaggtgtccg
      901 aacacctgtt gccgaagacc cgcgcgtatc acgagatctg gctcaatggc gagaagctcg
      961 cgggcccggc cgaagaagag ccgatcctcg ggcagctcta tctaccgcgc aagttcaaga
     1021 ccggaatcgt cgttccgccg cacaacgacg tggacgtgtt ctcgcaggat ctcggtttca
     1081 tcgccattct ggagaccgaa ggcctggagg aggggaagct gctcggcttc aacctttccg
     1141 tgggcggcgg actcggcgcc acgcatggcg agccgcagac tttcccgagg ctcgcggacg
     1201 tcatcggttt tctgaagcca gagcagatgc tcaaggtggc cgagaccgtg gtcatgatcc
     1261 agcgcgactt cggtgaccgc acggatcgca aacacgctcg cctcaagtac acgattgccg
     1321 accgcggcat cgactggttc aaggccgagc tcggcagacg cctgggctat gagctcgaac
     1381 ccgcccggcc gtacaacttc accacccagg gtgatcgctt cggatggctg cgcggtcacg
     1441 atggtcgatg gcatctcacg ctgcgcatcg aagccggccg cgttggcgac acacccggcc
     1501 agccgtttct caccggcctc gagaagattg cggcgatcca ccagggcgac ttccgcctca
     1561 ccgccaacca gaacctgatc atcgccaacg tgccggcgag tgagtgcatg aacatcgaca
     1621 agctcgtggc cgatcacggc ctgggcaagg cgctgttcac cacgccgctg cgccgcgatg
     1681 cactggcgtg cgtggcgctg cctacctgcg cgctggccat ggccgaagcc gagcgctatc
     1741 taccgaactt caccgatcgc gttgaagcgt tgctgttcaa acatgggctg cgcgagattc
     1801 ccctcacgct gcgcatcacc ggctgcccga acggttgcgc gcgtccgtac ctcgccgaga
     1861 tcgccctcat cggccgcgct ccaggccgtt acacgttgcg tctcggcgcc gacgccgtgg
     1921 ggtcgcggct gaacgtggtg taccgcgaca acatcgacga gaacgccatc gtcgccgtgc
     1981 tcgatgaact catcgcgcga tacgccgtcg agcggcacac acacgaacgc ttcggcgact
     2041 tcctgtggcg cgcaaacgtt ctcaggcagg agacccgcgc gtgatcgagt ccgtcgcgcg
     2101 cgaagccccc aaattcagca ccgctccggt gtcgctggtg ggtgcgccgc gcctgacgct
     2161 ttctgcgaag gaatttgccg gcgatccgga cgccgcgaat cgcatcaaca cctggctcga
     2221 gtccgtggac gccgccacgc gcgtgcgctg ggccctcgag aatctgccgg gcccgttcgc
     2281 gctgtcgtcg agcttcggcg cgcaggccgc ggtttccctg catctcgtgg cgcaggcgga
     2341 tcccgatatc ccggtgctgc tcatcgacac gggttatctg tttcccgaga cctaccagtt
     2401 catcaagcag ctcgagcagc aactctcgct gaacattcag actttctcga gcccgcagtc
     2461 ggtcgacgaa ttcgaagcgc agtacggcaa gctctgggaa cagggccggg aagggctcga
     2521 caaatatctc gagctgcgaa aggtggagcc catgcgccgc ggcctcgcaa cgcttggcac
     2581 cgccacgtgg ttcgcgggcc tgcgccgcag ccaggctgcg agccgcgccg cgctgagtcc
     2641 gctcgaagtg cgtgacggcc gcttcaaggt gcatcccatc tttgactggt ccgatcgcga
     2701 cgtgtacctg tacctcaagg cgcaaggact gccgtaccac ccgctgtggg acaagggata
     2761 cgtgtccatc ggcgactggc attccaccaa ggcgctgcac gaggtggagt cggcggatga
     2821 actgcgcttc tccgggctca aacgagaatg cggcattcac ggcctggaag gttgaaaagg
     2881 ccaccgacgc tttttcccgc acttcgaccg ggcgtcatcg tgaccccgtg caccgccagg
     2941 gcgcgagcgg cggcttcagg acaccggatc ggcatccatt ccgtggcaca gggattgcta
     3001 cttctcccat gcaggatatg accctcgaca aacccatcgt gttaggcgcg cgcgccagcg
     3061 tgctggcgca ggtgcaagcg cgcctcgtcg tcgatgcttt gcttgcgcgc catccgggtc
     3121 tgaagatcga gtattccttc ctcacgtccc ccggcgatcg cgagctcgac acaccgctgg
     3181 ccgagctcct gcgccgcggc ggcttcaccg gggatcttgg cgtggcactg cgcgccaacg
     3241 ccatcgacat cgcggtccat ctctggaagg acctgccgtt tgccggcaac ccgctgacgc
     3301 acatcgccgc gacgctgccg cgcgccgaca cgcgcgatct gctgctggtg aaacgaagct
     3361 ggctatcgcg ttcacagggc gagctgcgcg tgctgtcttc gtcggcgcga cggcgcacca
     3421 atctttcggg cttcctgcag tgggcgctgc ccattcaacc accgcgcgtc accttcgtgc
     3481 tggcgcgcgg ggattccgaa gcgcgcctcg cgcaactcat ggacggcgaa gccgcggcgt
     3541 tggtcgtggc caaggccaac atcgaccggc tgctcgagtc cacggcgcct gaattcgccg
     3601 gccttcgcga gcgcgtgcgt gccgcgctcg atgcatgcga agtcatggtg ttgccactcg
     3661 cgctgaatcc gaccgcgccg gggcagggcg cgctcgccat cgagatccgc cgcgatcgag
     3721 ccgacctcgc cgaactactg gcgcccatca acgacgaagc caccttcggc cgcgtgatcg
     3781 ccgagcgcgc gcggctttcg cccaccgggg atgaagacca tccgctcggg gtttccatcg
     3841 tccccatcca ttgcggagaa gtggagttca cgcgcggcca gaagggcggg cagtggatac
     3901 agagtgccga gctgcgccgc cacgatggtc ccctgccacc cgccagcagc caacacgcca
     3961 tctggaccgg cgacgaaggc agcgccgatc gctaccggcg tgtcgcgttg caggtgcgcc
     4021 acgatcagtt tgccgacgcc cgcgtcggcc tgttcgtagc ccggtccgaa gcctggcccg
     4081 acggcttcag tccccgcccc gggcaggtgg tctggagcgc cggaatcgac acgtggcggc
     4141 ggctcgccgc ccggggaata tgggtgcacg gcagcgacga gagccttggg gaaggcggct
     4201 ccggggccct tcgcagcttg ttcccgcgcg tccagcgctg ggtcaagttc tcccatgaac
     4261 agggctacga ctcgccgttc agcgagcgta ttcccaccta ccggctcgaa cgctgcgctc
     4321 ccttggccga cctgaccggc cgaacccact ttttctggcg cagcggggcc cagttttacg
     4381 actatctcca ggccaaccct gaaatccgtg gcgcctggca tggctgcgga cctggcaata
     4441 cattcagtat gatccgcgcc gccatcggtg aagaacgcgt ccgcgccttc ctgtcggcgg
     4501 atgtgtttcg ccaggaagta gagcaatgaa aagactgcaa cgattgcgcg ccaccgcggc
     4561 attgcgggac atggtgtccg agatcggctt tggccagagc cagctgatcc agcccctgtt
     4621 cctctccgag gcggcggtca aggaagaacc catcgccggg ctgcagggca cgtcgcgcca
     4681 tacgctgtcc tcgctgctca cgcagttcga caaggacctg ggcgccggcg tgcgccagtt
     4741 cctgctgttt cccgtgccag cgcacaagtc caaccgaagc ttccaggccg agttcgtcgg
     4801 cagcgcacta ggccagatgc gccagcgtgc gggcgacgat gcgaccatct gggtcgacac
     4861 ctgcctgtgc agcttcaccg agtccggcca ctgccaaata cacgacgctc gcggcaaaca
     4921 ggatctcgcc gccacgcaca gcgcattggc cgtgctcggc cttacctatg tgaaggctgg
     4981 tgccaatggc atagcaccct ccgacatgaa cgacggccgc gtcgcgcatc tgcgcgccac
     5041 gctcgatggt tccggtttcg acctcgcgcc catcatgagc tacagcacca agttcgcgag
     5101 caattactac ggaccgttcc gcgatgccgc cgactccgca ccgcagttcg gcgaccgccg
     5161 cagctatcag ctggacgtgc gcaatcgcag cgatgcgttg gcctccagcc gccgctgcgc
     5221 cgaagagggc gcggatctat tgatggtgaa gcctggccag ccgtccgctg acctgatccg
     5281 cccgattcac gagcaaaccg gtttgcccgt gggcgcctac caggtcagcg gcgaatacac
     5341 ctcgctggcg ttgttagctc gcgagaacta cgtcgaattc gacgccgcgc tgctcgagag
     5401 ctggtacaac ctgcggcgcg ccggcgccag ctacatcatc acttacggcg cgcgactcgc
     5461 gcagggcctg gggctgccac gctgatgcgt tacttcccgt tgttcctcga cataaaggac
     5521 aagccggtgc tgctcgtggg tggcgggaaa gtggctgcac gcaagttctc cttgttagtg
     5581 gatgccggcg ccaggatcac cgtcgtggct ccgcggctgg gtgacgaact caccgccgcg
     5641 ctcgcgcgtg gcgctgtcac gcatcacgcg cgcgagttca cccagaccga catcggcggt
     5701 gcctggctcg tagtcgccgc caccgatgat cgcgccgtga atgccgccgt ctccgctgcc
     5761 gccaacgccg cgcgcattcc gtgcaatgtc gtcgacgatc gcgagctctc cagcttcatc
     5821 atgccggcca tcatcgaccg ctcgcccgtg cagatcgcga tatccaccgg cggcacctcg
     5881 ccagtgctcg cgcgcctcat ccgcgaacgg ctggaaactc ttttagatag ctcgctgggc
     5941 cccctggccg cgttcgctga tcgctggcgc gtggccgtga aagccaggtt tgccgatatc
     6001 ggtgcacgcc gccggttcct ctcatggatg ctgaccggtc ccgtggccgc tgccgtacgc
     6061 gccgaacgcc atggccaggc tgaagaactc acacgcaagg cgctcgaaaa ggccgatggc
     6121 attcccgccg gtcacgtcgt gctggttggc gccggccctg gcaactccgg gttgatgacg
     6181 ctgcagggcc tgcgcgcact gcaagaggct gacgtcatcg tgcacgaccg tctcgtgtcc
     6241 gccgaagtga tggatctcgc gcgccgcgat gccgcccgct tcgacgtcgg caagttcgtg
     6301 ggcggcggcg gcgccaccca ggaagagatc aacaagctgc tcgtgga
//
LOCUS       DASQJS010000088         2458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_49590, whole genome shotgun sequence.
ACCESSION   DASQJS010000088 DASQJS010000000
VERSION     DASQJS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49590"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..941)
                     /locus_tag="VEW08_03490"
     CDS             complement(138..941)
                     /locus_tag="VEW08_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HYJ39827.1"
                     /translation="MRVQTFAPLFALFLFAAGAAQAQTAGVVTLNANQTSATGSLTPV
                     LTWSTNPVAQSCTASGGWSGTKAASGTQTLAAITASANYTLTCTWGTGSTRVSWTAPT
                     TNSDGSPLTNLARYKVYYGTSSTTLSSSATVDDITATSATISPLTPGTWYFAVRAVNT
                     ANVESANSNVASKAVSGAIAARSVAIAITAPPPPTTGYVTVSTNVWDVRRRSDGVWVR
                     IGVVGQIALGRPCSTTFKVGAYHYLVSRSYVTLTKTPKSTNLVTKCERL"
     gene            complement(1257..2258)
                     /locus_tag="VEW08_03495"
     CDS             complement(1257..2258)
                     /locus_tag="VEW08_03495"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HYJ39828.1"
                     /translation="MEKRAAGRAARFVFLAASSMLLAACVTREPFIPANLPPGAHSRV
                     ELESTPFFPQKKYHCGPAALAAVLGASSVDTTPEALVPIVYLPARRGSLQVEMQAAPR
                     KFDRLSYPLSRNLADMLAELNAGRPILVLHNYGLSFWPRWHYAVVIGYDSATDRFILR
                     SGQKRRAEWRARTFMVAWHHGGRWAMVVLRPGEIAASANPALYLEAAADFERGAAPAD
                     SRLAFDAAVQRWPTEPVAWIGRGTAEYRAGNFANAARDYAAAVRLDPDNIGARNNLAQ
                     SLLDLGCPGGAREQLAPVNVDALAPSLREAVLDTRQHIEAAVAASPVSDPSRCLPQP"
BASE COUNT          441 a          766 c          824 g          427 t
ORIGIN      
        1 acgcgttgct tgcaaaattc ccggagatcg acaagacccg tatcgaagcc gtgggtcgtg
       61 gctgggaaga acccacgggc accaccgacg cggcgcaaaa tcgccgggtc gaggtgcagt
      121 ggttcactgt tgaatagcta gagcctctcg catttcgtca cgaggttggt cgacttgggc
      181 gtcttcgtga gggtgacgta tgaccggctc accaggtagt ggtaggcgcc aaccttgaat
      241 gtcgtgctgc agggccggcc cagcgcgatc tgcccgacca cgccgatcct cacccatacg
      301 ccgtcggacc gccggcgcac atcccagacg ttggtgctga cggtgacgta gccggttgtc
      361 ggaggcggcg gcgccgtgat ggcgatggcc accgagcgcg ccgcgattgc acccgacacc
      421 gccttagacg ccacattgct gttggccgat tccacgttgg cggtgttgac cgcgcgcact
      481 gcgaagtacc aggtgccggg cgtgagtggg ctgatggtgg ccgaggtcgc cgtgatgtca
      541 tcgacggtgg cgctgctcga caatgtggtg ctgctcgtcc cgtagtacac cttgtagcgg
      601 gcgaggttcg tgagcgggct gccgtccgag ttggtcgtcg gcgcagtcca gctgaccctc
      661 gtcgaaccgg taccccaggt gcaagtcagt gtgtagttag cgctggcggt gattgccgcc
      721 aatgtctggg ttccggaagc ggccttcgtg cccgaccagc caccgctcgc ggtgcaactc
      781 tgtgccaccg gattggtcga ccaggtcagc accggcgtca agcttccggt cgcggaagtc
      841 tggttggcgt tgagcgtcac gacaccggcg gtctgcgcct gtgccgcgcc ggcggcgaat
      901 aagaatagag cgaataacgg agcgaatgtc tggacgcgca tgaataacga ccccacgagt
      961 ctggtaaaga agacgtggga gcacagtggg tttgcggtct tcgcgccgca aaccggaagc
     1021 cgctttctgc ggaaagacaa taggaggaat tctggcgctt tggtgcgaca cgtccgacgc
     1081 catttcggtc cgctcgtctg cgccggccga caaaaacacc gaaggtgtac aagaaacccc
     1141 cgaacgagtg ccggcgagcg cgaacttttt tcaatctcga caagcgtgcg agcagtgctt
     1201 gacccgacag cgcgggtttt gcttcgcggc catggtgatg gcagttggcg acaaactcag
     1261 ggttgcggca aacacctgct cggatcgctt acgggactgg cggctaccgc cgcttctatg
     1321 tgctggcggg tatcgagcac tgcctcgcgc agcgatggcg ccagtgcatc gacattcacc
     1381 ggcgccagct gctcacgcgc gcccccgggg cagccgagat cgagcaacga ctgcgcaaga
     1441 ttgtttcgcg caccgatgtt gtccggatca aggcgaactg cagcggcata gtcgcgcgcc
     1501 gcattcgcga aattgccggc gcgatattcc gcggtgcccc gccctatcca ggccaccggc
     1561 tctgtcggcc agcgctgcac ggccgcatcg aacgccaggc gagaatccgc tggagccgct
     1621 ccgcgttcga agtcggccgc ggcttcgaga tagagcgctg gattggcgct cgccgcaatt
     1681 tccccggggc gcagcactac catcgcccag cggccaccgt ggtgccaggc gaccatgaac
     1741 gttcgcgcgc gccactcggc ccgccgcttc tggccggagc gcaggatgaa ccggtctgtg
     1801 gccgagtcgt agccgatcac gacggcgtag tgccagcgcg gccagaacga cagcccgtag
     1861 ttgtggagca cgagaatcgg acgacctgcg ttcagctcgg cgagcatgtc cgccaggttg
     1921 cgcgacaacg ggtacgagag ccggtcaaac ttgcgcggcg ccgcctgcat ctccacctgc
     1981 aggcttccgc gccgtgccgg tagatagacg atgggtacca gcgcctccgg cgtggtgtcc
     2041 acgctcgagg cgccgagcac cgccgccagc gcggccggac cgcagtggta ctttttctgg
     2101 ggaaagaatg gcgtcgattc gagctcgacg cgggaatggg caccgggcgg aagattcgcg
     2161 ggaatgaatg gctcacgcgt gacacacgcg gccagcagca tcgaggaagc tgcgaggaaa
     2221 acgaagcgcg cagcccgtcc ggctgcgcgc ttctccatca gtccttcttc ccggggaatg
     2281 ggctcaggct ttcttgaaga tgtcgatgac gccggtcagc tcgaggatga tcagcacgat
     2341 gaacaccgcg ccgacgatcc agacggcatc ggcaccaccg gcgggcgcgt tctgcatgtc
     2401 agcagcaagc ttgtgcagtt cgcgatcgtt gagcctggcg aggcggcggt cgaccgcc
//
LOCUS       DASQJS010000089        15742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2875802, whole genome shotgun sequence.
ACCESSION   DASQJS010000089 DASQJS010000000
VERSION     DASQJS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15742
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2875802"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..270)
                     /locus_tag="VEW08_03500"
     CDS             complement(91..270)
                     /locus_tag="VEW08_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39829.1"
                     /translation="MRIAKPMLLVLTPIGVVWGIVEAARFHWWLAVLMGALVSVIGLF
                     TWMTVKRIRAERAPP"
     gene            complement(274..1602)
                     /gene="hslU"
                     /locus_tag="VEW08_03505"
     CDS             complement(274..1602)
                     /gene="hslU"
                     /locus_tag="VEW08_03505"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HYJ39830.1"
                     /translation="MTPREIVAELDKHIVGQGAAKRSVAIALRNRWRRMQIENPLRQE
                     ITPKNILMIGPTGCGKTEIARRLARLAKAPFVKVEATKFTEVGYVGREVDSIIKDLAE
                     VAVKMTRDEEIAKVRDRAADAAEERVLDALLPQPKMMGFSTDAEPSPRDQETRQKFRK
                     MLREGALNDREIELELRAIPAGVQIMAPPGMEEMQEQLSSMFANLGGGRTKSRKLKVK
                     EAQKLLIEEEAGKLVNEEELRARALANAEQNGIVFIDEIDKIARRQETMGADVSREGV
                     QRDLLPLVEGSTVSTKYGQIKTDHILFIASGAFHMSKPSDLIPELQGRFPIRVELNSL
                     GVDDFVRILTEPDASLTAQYKALLGTEQVDVTFAKDGVRRIAEIAFSVNEKTENIGAR
                     RLHTVMERLLETVSFDAAENGGSKVTIDAAYVEQHLGNLAKDEDLSRYIL"
     gene            complement(1602..2144)
                     /gene="hslV"
                     /locus_tag="VEW08_03510"
     CDS             complement(1602..2144)
                     /gene="hslV"
                     /locus_tag="VEW08_03510"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HYJ39831.1"
                     /translation="MSEVFNPHGTTILAVRRNGAVSIGGDGQVTLGNTVMKGNARKVR
                     PLGNGKVLGGFAGGTADAFTLFERFEGKLDKFGNLTRAAIELAKDWRSDRYLRRLEAL
                     LLVGDTKNLFVISGNGDVIEPDHDVVAIGSGGPFAQAAARALIENTELDARSIVERSL
                     NIAADICIYTNRNLVIEELK"
     gene            complement(2166..3062)
                     /gene="xerC"
                     /locus_tag="VEW08_03515"
     CDS             complement(2166..3062)
                     /gene="xerC"
                     /locus_tag="VEW08_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008040629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HYJ39832.1"
                     /translation="MPDRSLIDGYLKHLGTERRLSPHTASNYARDLRALADFMERGSL
                     PDWGKVDSQHVRVFAARAHAGGLSPRSVQRRLSAVRGFFNYLVRERLVASNPAVDIRA
                     PKAARRLPGTLDVDQLNQLLDIPAEDALAVRDKAIMELFYSSGLRLDELVGLDIAQLD
                     LADRTVRVLGKGRKTRVVPVGRKAVDALRTWLRERATLADVDDPALFVGRNGSRLKHR
                     AVQLRIAYWARRKGLPSHVHPHLFRHSFATHLLESSKDLRGVQELLGHADISTTQIYT
                     HLDFAHLARTYDASHPRAKRKT"
     gene            complement(3101..3808)
                     /locus_tag="VEW08_03520"
     CDS             complement(3101..3808)
                     /locus_tag="VEW08_03520"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HYJ39833.1"
                     /translation="MSTQTARGLAAGQTDEERIERYLSLNPDFFERHQPLLARMRLPH
                     MRTGSTVSLVERQVEVLREQKTEADRRLAEFIAVARANDTLAERIHRFTRRMLRAPNP
                     VVAISTLEASLREDFDAFHSVLVLTAPIASLANVEAEPFLRKLPADDANIRTFEALLA
                     TGKPRCGQVRDTQRDFLFGPESASIGSVALVPLGDAGALGLLALGSAERERFHPGMST
                     EFLKRMGELITDAMSRP"
     gene            complement(3825..4661)
                     /gene="dapF"
                     /locus_tag="VEW08_03525"
     CDS             complement(3825..4661)
                     /gene="dapF"
                     /locus_tag="VEW08_03525"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HYJ39834.1"
                     /translation="MQLEFTKMHGLGNDFIVFDAPSDGWLPTATQWRALAARHTGIGF
                     DQALVLEPARRAGTDVYYRIFNADGGEVEQCGNGVRCLASFLHRRGNVTNSKGEIVLD
                     SPAGPIHARIHDANLISVDMGVPNFDPKSLPFEASAEAHVYPLTVAGTEVEIGAVSMG
                     NPHAVLTVTSVASAPVDRLGPAIERHPRFPKRVNVGFMEIIDRARIRLRVFERGVGET
                     LACGTGACAAVAIGRRHGRLDPTVEVELPGGKLEITFSAPGEHIWMKGPTAVSFTGRV
                     EI"
     gene            4692..5285
                     /locus_tag="VEW08_03530"
     CDS             4692..5285
                     /locus_tag="VEW08_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39835.1"
                     /translation="MRLAPVAVAALAIFAGYQLYSRREISHAPGVLVAEDPEQRVIES
                     AAVIERGEFRLRPRAEFSATVRVLRREDYSMGPLAKLVPTDFAVGWGPMSDSAVLADV
                     EISQGNRFYFWRTENWPIERAEIETHSANWHVIPENPTVSGVLGRLRAGSVVELRGQL
                     VDIEGGEGGMRTSLTRGDTGAGACEILLAESVRITGQ"
     gene            5302..5850
                     /locus_tag="VEW08_03535"
     CDS             5302..5850
                     /locus_tag="VEW08_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ39836.1"
                     /translation="MNADVVSHAAAMRIRRAVAADADALAEIGARTFFETFAADNTAA
                     DMRLFLESTWRPDLQRAEILDADLDTLLVCDATGAIAGFAQLREAHPPAGIAVSAPVE
                     LKRFYVDKAWHGRGLALTLMGASELAARARGARELWLGVWERNERAQAFYRKCGFRKV
                     GTTVFVVGTDPQTDNVMLRELA"
     gene            5847..6245
                     /locus_tag="VEW08_03540"
     CDS             5847..6245
                     /locus_tag="VEW08_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ39837.1"
                     /translation="MSVRIEHVGVWVADIDRVAAFYVKYFGAQVGNLYRNASKGFESR
                     FLTFAGGARLEVMTRQDVTGRAANEQLGLAHVALSVGDEAAVDALAARLRADGVTPDS
                     GPRRTGDGYYECVVRDPEGNRVEIAAGNPV"
     gene            6242..8806
                     /locus_tag="VEW08_03545"
     CDS             6242..8806
                     /locus_tag="VEW08_03545"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HYJ39838.1"
                     /translation="MKSLCWSVLLCVFAPFALAQEPASAWPEVTGSAKPWTRWWWPGS
                     AVDKAHLTAQLEAIAAAGIGGVEITPIYGPRGSEARYVDFLSPQWMEMLEHTTREATR
                     LGLSVDMATGTGWPFGGSTVSTADGSSSLALVDGKLVGKPTAMKVKRAAPGGEGLVLD
                     PYSTAALDRYLRPFSKAFTRVSRQGMRAQFHDSFEYYNASWTQALPEVFRKMHGYDLQ
                     SYAAAFSGEPTLDDDTMSRIKGDYRRTLAKLHLDYVNAWVTWAHEEGFQARNQAHGAP
                     GNLLDLYGAADIPETESFGLTELPIVGLRGDAVGVSVDPDPPRVTIGRFASSAAHVMG
                     RPLVSSETLTWLRENFRESPAAAKPQIDRLFAAGINHIFYHGTVYSPADAEWPGWFFY
                     ASTQLTPANPLWDDFGAMHAYITRVQSVLQAGAPDNDILLYWPFDDMVDHSTGLMEQY
                     GVHENKWLTESSTGRVAADLLWAGYAFDFVSDAQLMRLKVDGKTFIAPGGGRYRAIVV
                     PGTRRMSVETLTQLRELAKRTGRVIIESLPQDVPGYSRLEARRTEFRKLATAPDLTRA
                     VVGDQIEAALMKHGVPREQASPGLVHIRRARTDGYDYFFANLTGKAFDGWLQLGVSAE
                     SAMILDPLTGEAGTAAVKRGAEGRLQVYLQLESGQSMLVSTYRTKGKRPAQPWRYTKS
                     SGDGIAVQGEWSLEFIKGGPVLPGPARMRSLSSWTALDDPEAKRFAGTARYRVEFDAP
                     AKAADAWQLDLGEVRETARVTLNGKRLATAWSLPFKLHVDGLKARGNVLEVEVTNLAA
                     NRIRDLDVRKVPWKIMREINIVTVKYRPFDAAAWEVEPAGLLGPVSLIPLSLVRPR"
     gene            complement(8793..9212)
                     /locus_tag="VEW08_03550"
     CDS             complement(8793..9212)
                     /locus_tag="VEW08_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HYJ39839.1"
                     /translation="MFENPTKPDIDTFLRRIRTVAILGLSETPGRPSHNVAKALQKFG
                     YRIVPVNPTATEILGVRAWPDLDTAIAGAGPIDVVDVFRRPEHVAGIVEDCIRLKVPA
                     LWLQDGVIDETAAQKARDAGIFTVMNRCMFRDRSTLA"
     gene            9268..10197
                     /locus_tag="VEW08_03555"
     CDS             9268..10197
                     /locus_tag="VEW08_03555"
                     /inference="COORDINATES: protein motif:HMM:NF021454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HYJ39840.1"
                     /translation="MRLVNNLANITAAWMICAAFTVPVFAQDSPEPEQDLASEESIAG
                     EEEALPEFKYSTGDILLPNKVATLHLGEKYHYLAPDEANKLLQAWGNPPDDETQGAIL
                     PAGVHPLGEGGWAVFLTYTDDGHIDDSDAQEIDYDDMLKDMKESIEDSNEARKQAGYG
                     EMHLVRWAEPPRYDAAGKKLYWAKEISGASGEMHSLNYDVRVLGREGVLSMEAVAPMD
                     LLAQVRTDMRPLLEVAEFNEGYRYAEFNAKTDRVAEYGLGALIAGVAATKLGLFAKLG
                     ALLAPFWKFIIIGFAALGGFLAKIFGKKKDEAI"
     gene            10230..10787
                     /locus_tag="VEW08_03560"
     CDS             10230..10787
                     /locus_tag="VEW08_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYJ39841.1"
                     /translation="MEPGGHDIRVPPGENVLTAALAAGIPLPFSCRAGRCASCKAKLL
                     TGSIEYPTGLPPGITKAEAERGEVLLCQARPRSDLSIESRRVPVRVSNAASCEIVAIE
                     PLALRALLLRLRFVEGKVDAHPGQFVDVRNHAGDAERLAVIGTGTGVVELECGDDGSL
                     LRAWLDSSAVHGSILRVAGPFDRPR"
     gene            complement(10794..11531)
                     /locus_tag="VEW08_03565"
     CDS             complement(10794..11531)
                     /locus_tag="VEW08_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017902004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HYJ39842.1"
                     /translation="MSLRVLIVDDEPPARERLSSMLAEAGDFVLAGEAGNGEQAIDMV
                     DQLTPDIVLLDVRMPGIDGLEVARHLATLPAPPAVIFTTAFDEYALQAFDSEAVAYLL
                     KPIRAEKLRAALAKAERLSRAQLQQVATATRTAAHRSHIGVRGRDGLRLIPIEEVFCF
                     QADQKYTTVKHLKGEDLIEDSLKTLEEEFATNFVRIHRNALVNTKYLERIARDASGQH
                     FVHLRGLPDALEVSRRMAGDLKDRFRI"
     gene            complement(11528..12592)
                     /locus_tag="VEW08_03570"
     CDS             complement(11528..12592)
                     /locus_tag="VEW08_03570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYJ39843.1"
                     /translation="MNHPPQYPAAPGEGPRQLYLPNFCSASNVLIIILICELTALLLA
                     LARNEAALGFWSDLGLTSMFLLWIALCGAALLCLLRDYLSRQSVAVSAAMVLTMTVTL
                     VTVVSAVTYYFGFGLLQDQHWSFLARNAGITFVVTALALRYFYVTHEWRHNVELQAKA
                     RVHALQARIRPHFLFNSMNTIAALTRSNPARAEEAVLDLAELFRANLNEKRGQIPLAE
                     EIDVARTYQRMEQLRLGDRLRVDWKVDSLPTDALVPGLTLQPLLENAIYHGVEPRPGG
                     GVVTVTGEFNKGMITIVVRNPVPLPSLTVRDGNRLALANIKERLGLMYGDRATVKAGR
                     FDEEYIVTLRFPFTTSVADA"
     gene            complement(12589..13476)
                     /locus_tag="VEW08_03575"
     CDS             complement(12589..13476)
                     /locus_tag="VEW08_03575"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ39844.1"
                     /translation="MKRVGFAGLGAMGAPMAKNLHRAGLLEAVWNRSSDKAVALAAEL
                     GCQSPATLADLAAMVDVVVVCVSADADVRSVVEGLGPGLRKGALVIDCSTVSAETARW
                     AEEFLSARSAQFIDAPVSGGVEGAQKGTLAIMTGGDPAAFEAARPILAAMGKTIEHFG
                     PAGSGQATKATNQIMCAGIIRACAEAMAFAGAHQLPLDRVVSTLGAGAGSSWYFVHRA
                     PNMVRGSYPAGFRIKLHAKDLRICRDMAARFGVSLPVVDSMLAEYAELMEQGYGDEDI
                     SATHRLKQALFEDAAKHKA"
     gene            complement(13567..14055)
                     /locus_tag="VEW08_03580"
     CDS             complement(13567..14055)
                     /locus_tag="VEW08_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HYJ39845.1"
                     /translation="MRHMKILVVITGVLACALFNSGLAQAGDVYKYVDERGTTLYTDK
                     PIPGAILVSTGSQRPPEVAQRNYAANQAATNSQLNASNQRITDSQDNSRVAATVAKDL
                     EASRAERCKKARETYNSSISSHRMYRTGKNGEREYLSEAELAQQRMDAARAVEAICGP
                     QG"
     gene            complement(14233..15657)
                     /locus_tag="VEW08_03585"
     CDS             complement(14233..15657)
                     /locus_tag="VEW08_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HYJ39846.1"
                     /translation="MHQDKKRTKGSSVMRGLGALLLMLPALVFGQDAPPPPNKGDISW
                     LMTSTVIVILMTIPGLALFYGGMVRSKNVLSLLMQVMIIFCLGILMFAIYGYSVAFTE
                     GPNAFFGGFSKMFLKGMTGDSITGTFSKATNIPELGFFVFQATFAAITPGLIVGALAE
                     RVKFSAILVFIILWLTFSYYPIAHMVWFWSGPDAYTTAEAGAAANVNAGFLFNKGALD
                     FAGGTVVHINAGIAGLVGALLLGKRIGFGTTALPPHNLPMVMIGASLLWVGWFGFNVG
                     SNLEANGFATLVFTNTLLATAAATLAWIGAEWIIKGSPTMLGAASGAVAGLVAITPAC
                     GWVGTMGSIAIGAVAALICVWACSGLKKMLGYDDSLDVFGVHCVGGIVGAILTGVFND
                     PALGGTDVYDYVANAYPDPAAYDMGAHVVSQLWGVGVTLLWSGLVSLIAFKLVDLTIG
                     LRVNEEQEREGLDTVTHGERAYNG"
BASE COUNT         2771 a         4898 c         5261 g         2812 t
ORIGIN      
        1 cgtttccggt gaccggaagt acggactcga ggacggtcag gatcggatcc ttgttgcgtt
       61 ccgccgcggc tgacgtcagg agctcgttgg tcacgggggc gcgcgctcgg cgcggatgcg
      121 cttcaccgtc atccacgtga acaggccgat gacgctgacc agcgccccca tgagcacggc
      181 cagccaccag tggaatcggg ccgcttcgac gatgccccag acgacgccga tgggcgtcag
      241 gaccagcagc atgggttttg caatcctcat ggcctagagg atatagcgac tgaggtcctc
      301 atccttggcg agattgccga gatgctgctc aacgtacgcc gcatcgatcg taaccttcga
      361 gccgccattc tcggccgcgt cgaacgacac ggtttcgagc agccgctcca tgacggtatg
      421 cagccggcgc gcgccgatgt tctcggtttt ctcgttgacg ctgaaggcga tctcggcgat
      481 gcggcgcacg ccgtccttcg cgaaggtcac gtccacctgc tcggtgccca gcagcgcttt
      541 gtactgggcc gtcagcgacg cgtccggctc ggtgaggatg cgcacgaagt cgtccactcc
      601 aagcgaattg agttcgacgc gaatcgggaa ccggccctgc aactccggaa tcagatccga
      661 gggcttcgac atgtggaacg cgcccgaggc gatgaacagg atgtggtcgg tcttgatctg
      721 tccgtacttc gtggacacgg tgctgccctc gaccagcggc agcaggtcgc gctgcacgcc
      781 ttcgcgggac acatcggcgc ccatggtttc ctggcggcgc gcaatcttgt cgatctcgtc
      841 gatgaacacg atgccgttct gctcggcatt cgcgagcgcc ctggcacgca gttcttcttc
      901 gttgacgagc ttgccggctt cctcttcgat gagcagcttc tgcgcctctt tcaccttgag
      961 cttgcgtgat ttggtgcgac cgccaccgag gttggcgaac atgctcgaaa gctgctcctg
     1021 catttcctcc atgccgggcg gcgccatgat ctgcacaccg gctggaatgg cgcgcagctc
     1081 gagctcgatt tcgcggtcat tcagcgcgcc ttcgcgcagc atcttgcgga atttctggcg
     1141 cgtctcctga tcgcgcgggc tcggctcggc gtcggtggag aagcccatca tcttgggctg
     1201 gggcagaagc gcatcgagta cacgctcttc ggccgcgtcc gccgcgcgat cgcgcacctt
     1261 ggcgatctct tcatcgcgcg tcatcttcac cgcgacctcg gcgagatcct tgatgatgga
     1321 atccacctcg cggccgacgt agccgacctc ggtgaacttg gtagcttcca ctttcacgaa
     1381 cggcgccttc gcgagccgtg cgagccggcg ggcgatctcg gtcttgccgc agcccgtggg
     1441 gccgatcatc aggatgttct tgggcgtgat ttcctggcgc agcggatttt cgatctgcat
     1501 gcggcgccag cgattgcgca gcgcgatggc gaccgagcgc ttggcagcgc cctggccgac
     1561 gatgtgtttg tcgagttcgg caacgatttc gcgaggagtc attacttgag ctcctcgatg
     1621 acgaggttcc ggttcgtgta aatgcagatg tcggcggcga tgttgagcga gcgctcgacg
     1681 atgctgcgtg catcgagctc ggtgttctca atcagcgcgc gcgccgctgc ttgcgcaaac
     1741 gggccgcccg agccgatggc caccacgtca tggtcgggtt cgatgacgtc gccgttgccg
     1801 gagatcacga acaggttttt cgtatcaccc acgagcagca gcgcttcgag gcgccgcagg
     1861 tagcggtcgc tgcgccagtc cttggcgagt tcgatggcgg cgcgcgtgag attgccgaac
     1921 ttgtcgagct tgccttcgaa gcgctcgaac aacgtgaagg cgtcggccgt gccaccggcg
     1981 aaaccgccga gcaccttgcc attgccgagg ggccgcacct tgcgagcgtt gcccttcatt
     2041 acggtgttgc cgagggtgac ctgtccgtcg ccgccaatgg agacggcgcc gttgcgccgc
     2101 acggccaaaa tcgtggtgcc gtgcggattg aaaacttcgc tcatgagagc ctgaaccttt
     2161 aagacctacg tcttgcgttt cgcccgaggg tgggaagcgt cgtaggtgcg ggccaggtgg
     2221 gcgaaatcaa ggtgcgtgta gatctgcgtc gtggagatat cggcgtggcc gagcagttcc
     2281 tggacgccgc gtaaatcctt actcgattca agcagatggg tggcgaacga atgacggaat
     2341 aggtgcggat ggacgtggct cggcaggccc ttgcgccgcg cccagtacgc gatgcgcagt
     2401 tgtaccgcgc gatgtttcag gcgcgagccg ttcctgccca cgaagagcgc gggatcgtcc
     2461 acgtcggcaa gcgttgcgcg ctcacgcagc caggtgcgca gcgcgtccac ggctttgcgg
     2521 cccacgggta cgacgcgtgt cttcctaccc ttgcccagca cacggactgt gcgatcggcg
     2581 aggtcgagct gggcgatgtc gaggccgacc agttcatcga ggcgcagacc ggatgaatag
     2641 aacagctcca tgatcgcctt gtcgcgcacc gccagcgcat cctccgccgg gatgtcgaga
     2701 agctggttga gctggtcgac atcgagcgtg ccgggaaggc gcctcgcggc cttgggtgcg
     2761 cggatgtcga ccgccggatt gctggccacg aggcgctcgc gcacgaggta gttgaagaag
     2821 ccgcgcaccg ccgacaggcg gcgctgcacg ctgcgtgggc tcaggccccc ggcatgtgcc
     2881 cgggcggcga acacccgtac gtgctggctg tcgaccttgc cccagtccgg gagactgccg
     2941 cgctccatga aatcggcgag cgcgcgcagg tcgcgcgcgt agttagatgc ggtatgcggg
     3001 gagagacgcc gctcggtgcc gaggtgtttc aggtacccgt cgatgaggct tcggtcgggc
     3061 atataagagg ggacaggctg agagcctgtc ccctggctaa ctagggtctc gacatcgcgt
     3121 cggtgatcag ttcacccatg cgtttcagaa actccgtgct catgcccgga tggaagcgct
     3181 cgcgttcggc actgccgagc gccagcaggc cgagcgcacc cgcgtcaccc agcggcacca
     3241 gcgccacgga gccgatgctc gcgctttcgg ggccgaacag gaaatcccgc tgcgtatcgc
     3301 gcacctggcc gcaacgcggc ttgccggtgg cgagcagtgc ctcgaacgtg cggatgttgg
     3361 cgtcgtcggc gggcagcttg cgcaggaacg gttcggcttc gacgttggcc agagaggcga
     3421 tgggtgcggt gagcacgagt accgaatgaa atgcgtcgaa gtcttcacgc aggctggctt
     3481 cgagcgtgct gatggccaca accgggtttg gcgcacgaag catgcggcgc gtgaagcgat
     3541 gaatacgttc ggcgagggtg tcgttggcgc gcgcgacggc gatgaattcg gcgaggcggc
     3601 gatccgcttc ggttttctgt tcacgcaaca cttccacctg gcgctcgacc agcgagacgg
     3661 tggagccggt gcgcatgtgc ggcagtcgca tgcgcgcgag cagtggctgg tgacgttcga
     3721 agaagtcggg gttcaggctg aggtagcgct cgatgcgctc ttcgtcagtt tgacccgcgg
     3781 ccagaccacg tgcggtttgc gtgctcatgc tttattcatc tcccctagat ttccacccgg
     3841 ccggtgaaag atacagcggt cgggcctttc atccaaatat gttcgccggg cgcggaaaag
     3901 gtgatctcga gttttccacc cgggagttcg acttcgactg tgggatcgag acgcccgtgc
     3961 cgtctgccga tcgcgacagc ggcacatgcg cccgtgccgc aagcgagcgt ctcgccgaca
     4021 ccacgttcga acacgcgcag ccggatgcgc gcgcggtcga tgatttccat gaacccgacg
     4081 ttcacccgct tcggaaagcg cggatgacgt tcgattgccg gcccgaggcg gtcaacgggc
     4141 gcgctggcga ccgaggtgac cgtgagcact gcgtgcggat tgcccatgga caccgcgccg
     4201 atctcgacct cggtgcccgc gaccgtcagc ggatagacgt gcgcttcggc ggaagcctcg
     4261 aagggcaggg acttcggatc gaaattgggt acgcccatgt cgaccgagat gaggttggcg
     4321 tcgtgaatgc gcgcgtgtat cggcccggcg gggctgtcga gcacgatttc acccttgctg
     4381 ttggtgacgt ttccgcggcg gtgcaggaag gaagccaggc agcgcacgcc atttccgcat
     4441 tgctccactt cgccgccatc ggcgttgaag atgcggtagt agacgtcggt gccggcgcgc
     4501 cgcgccggtt cgagcaccag cgcctggtcg aagccgattc cggtgtggcg ggcggccagc
     4561 gcacgccact gcgtggcggt gggcagccag ccgtcgcttg gcgcatcgaa cacgatgaag
     4621 tcgtttccga ggccgtgcat tttggtgaac tcgagctgca tggcgcgaag aataccttac
     4681 gatccgtcgc catgcgtctc gctcccgtcg ccgtggccgc gctcgcgatt ttcgcgggtt
     4741 accagcttta ttcacgtcgc gaaatcagcc acgcgccggg cgtgctggtg gcggaagacc
     4801 ccgagcagcg cgtcatcgag tccgccgccg tcatcgagcg cggcgaattt cgcctgcgtc
     4861 cgcgcgccga gttcagcgcc acggtgcgtg tcctgcgccg cgaagactac tcgatgggtc
     4921 cgctcgcgaa actcgtgccg acggatttcg cggtggggtg ggggccgatg tccgacagcg
     4981 ccgtgctcgc cgacgtcgag atcagccaag ggaatcggtt ctatttctgg cgtacggaga
     5041 actggccgat agaacgcgcc gaaatcgaga cgcattcggc caactggcac gtgatcccgg
     5101 aaaaccccac ggtgagcggc gtactcggcc gcctgcgtgc cggaagcgtg gtggaactgc
     5161 gcggtcagct cgtggacatc gaaggcggtg aaggcggcat gcgcacctcg ctgacgcgcg
     5221 gcgacacggg cgccggcgcg tgcgagatac tgctggccga gtccgtgcgc atcacggggc
     5281 agtgagtcgg gagacgtgaa gatgaacgcc gatgtagtta gtcatgccgc ggccatgcgc
     5341 atccggcgcg ccgtggccgc cgacgccgac gcacttgcgg agatcggcgc gcgcaccttc
     5401 ttcgagacct ttgccgccga caacacggcc gcagacatgc ggctgttcct ggaatcgacc
     5461 tggcgtccgg acctgcagcg cgccgaaatc ctggacgctg acctggatac gctgctggtg
     5521 tgcgacgcaa cgggggcgat cgcggggttc gcgcagctgc gcgaggcaca cccgccagcc
     5581 ggaatcgccg tgagcgctcc ggtggagctc aaacggttct acgtcgacaa agcctggcac
     5641 ggccggggcc tggcgctcac gctcatgggg gcttccgagc tggcagcccg ggcgcgcgga
     5701 gcgcgcgagt tgtggctggg tgtctgggag cgcaacgagc gcgcgcaggc gttctaccgc
     5761 aagtgcggct ttcgcaaggt cgggacgacc gtcttcgtcg tcggcacgga tccgcagacc
     5821 gataacgtga tgcttcggga gctcgcatga gtgtgcgaat cgaacacgtg ggcgtgtggg
     5881 tggccgacat cgaccgcgtg gccgcgttct acgtgaaata tttcggtgcg caggtgggca
     5941 atctctaccg caatgcgagc aagggattcg aatcgcgttt ccttacgttc gctggcggcg
     6001 cccgactcga agtcatgacg cgccaggacg taaccgggcg tgcggcgaac gagcagcttg
     6061 gccttgcgca cgtggcgctc agcgtcggcg acgaagcggc cgtcgatgcg ctggccgcgc
     6121 gcctgcgggc cgatggcgta acgcccgaca gcgggccgcg ccgtaccggc gacggctact
     6181 acgaatgcgt ggtgcgggat ccggaaggca atcgagtcga gatcgcggct gggaacccgg
     6241 tatgaaatca ctgtgctggt cggtcctgtt gtgtgtgttc gcgccgttcg cgctggcgca
     6301 ggaaccggct tcggcctggc cggaggtgac cggcagcgcg aaaccctgga ctcgctggtg
     6361 gtggccgggc agcgcggtcg acaaggcgca tctcacggcg cagctggaag cgatcgcggc
     6421 ggcgggcatc ggcggcgtgg agatcacccc tatctacggt ccgcgcggca gcgaggcacg
     6481 ctacgtggac ttcctgtcgc cacagtggat ggaaatgctc gagcacacga cgcgtgaggc
     6541 cacgcgcctg gggctgagcg tcgacatggc taccggtacc ggctggcctt tcggcggttc
     6601 cacggtcagc accgccgatg ggtcgagcag cctcgccctg gttgacggaa aacttgtggg
     6661 caagccaact gcgatgaagg tgaagcgggc ggcgccaggt ggcgagggcc tggtgctcga
     6721 tccctattcg acggccgcgc tcgatcgtta cctgcgcccc ttctcgaagg cattcacccg
     6781 ggtttcccgg caaggcatgc gggcccagtt ccacgattcc ttcgagtact acaacgcgag
     6841 ctggacacag gcgctgcccg aggtgttccg gaagatgcac ggctacgatc tccagtcgta
     6901 cgccgcggca ttctcgggtg agcctacgct cgacgacgac acaatgagcc gcatcaaggg
     6961 cgactaccgc cgcactctcg cgaagctgca tctcgactac gtgaatgcgt gggtgacctg
     7021 ggcacatgaa gaaggattcc aggcgcgcaa ccaggcacac ggcgcacccg gcaacctgtt
     7081 ggacctgtac ggcgccgcag acattcccga gaccgaatcc ttcggcctga ccgagctgcc
     7141 gatcgtcggc ctgcgtggcg acgcggtcgg tgtgagcgtc gatcccgatc caccccgggt
     7201 gaccatcggg cgtttcgcct cgtcggctgc gcatgtcatg gggcgcccgc tggtttcgag
     7261 cgaaacactg acctggctgc gcgagaactt ccgcgaatca cccgccgcgg cgaagcctca
     7321 gatcgaccgg ctgttcgctg cgggcatcaa tcacattttc tatcacggca ccgtgtattc
     7381 gcccgctgac gccgaatggc cgggctggtt tttctatgcg tcgacgcagc tcacgccggc
     7441 caatccactg tgggatgact tcggcgcgat gcacgcatac atcacacgcg ttcagtcggt
     7501 cctgcaggcg ggcgctcccg acaacgacat cctcctgtac tggccgttcg acgacatggt
     7561 cgatcacagc accggactga tggagcagta cggcgtgcac gagaacaagt ggctgaccga
     7621 atcttccacc ggccgagtgg ccgcggatct gttgtgggcg ggctacgcgt tcgatttcgt
     7681 ttccgacgcg cagttgatgc gcctgaaagt ggacggcaag accttcatcg ctcccggtgg
     7741 aggtcgttac cgtgccatcg tggtccccgg cacacgccgc atgtcggtgg aaacgctgac
     7801 ccagctgcgc gagctcgcga agcgcaccgg gagggtcatc atcgaatccc tgccgcagga
     7861 cgttcccggc tattcgcggc tcgaagcgcg tcgtaccgaa tttcgcaagc tcgcgaccgc
     7921 accggacctg actcgtgccg tcgtcggaga tcagatcgag gcggcgctga tgaaacacgg
     7981 cgtgccacgc gaacaggcgt cgccgggtct cgtgcacatc cgccgcgcgc gcaccgacgg
     8041 ctacgactat ttcttcgcca acctgaccgg caaggcattc gacggttggc tgcagttagg
     8101 cgtctcagcc gagtccgcga tgattctgga tcccttgacc ggcgaggccg gaacggcggc
     8161 ggtgaagcgc ggcgcagaag gcaggcttca ggtctatctg cagctcgagt ccgggcagtc
     8221 gatgctcgtg agcacgtatc gtacgaaggg taagcggcca gcgcagccgt ggcgctacac
     8281 gaagtccagt ggagacggaa tcgcggtgca gggcgagtgg agcctcgagt tcatcaaggg
     8341 agggccggtg ttgccgggtc ccgcgcgcat gcgctctctc tcgagctgga ctgcactcga
     8401 tgaccccgag gcaaaacgct tcgcaggtac cgcgcgttat cgagtggagt tcgacgcacc
     8461 cgcaaaggcg gccgacgcgt ggcagctcga tctgggcgag gtgcgcgaaa ccgcgcgcgt
     8521 gacgctcaat ggcaagcgcc tcgcgacggc gtggagcctg cctttcaaat tgcatgtcga
     8581 cggactcaag gcacgcggca acgtgctgga ggtggaagtc acgaacctgg ccgcgaaccg
     8641 catccgcgac ctcgacgtgc gcaaggtgcc gtggaagatc atgcgcgaga tcaacatcgt
     8701 caccgtgaag taccggccgt tcgatgccgc cgcctgggag gtggaaccgg ccggcctgct
     8761 cgggccggtc tcgctgatcc cgctgtcgtt agttaggcca aggtagaccg gtcgcgaaac
     8821 atgcagcggt tcatgaccgt gaagatgccg gcgtcgcgcg ccttctgtgc ggcggtctca
     8881 tcgatgaccc cgtcctgcag ccacagcgcc ggcaccttca ggcgaatgca gtcctcgacg
     8941 atgccggcaa cgtgttccgg ccggcggaag acgtcgacca cgtcgatggg gcccgcaccc
     9001 gcgatcgcgg tatccaggtc cggccaggcg cgtaccccga ggatctccgt ggcggtggga
     9061 ttcaccggca cgatgcggta gccaaacttc tgcagcgcct tggccacgtt gtggcttggc
     9121 cggcccggcg tctcggaaag accaaggatg gcgaccgtgc ggatgcggcg caggaaagtg
     9181 tcgatgtccg gtttcgtggg gttctcgaac atggttttgc ctcgggttgt gctagcgttc
     9241 ccccggaatt acctgggagg gttatcgatg cgacttgtca ataatctggc caatataacg
     9301 gctgcgtgga tgatctgcgc cgcgtttacg gtccccgtgt ttgcgcagga ttcgccggag
     9361 cccgagcagg acctggcgtc cgaggagtcc attgccggcg aagaagaggc gctgccggag
     9421 ttcaaatatt ccacgggcga catcctgctg cctaacaagg tggccacgct gcatctgggt
     9481 gagaagtacc actatctcgc ccccgatgaa gcgaacaagc tgctgcaggc atggggcaat
     9541 ccgccggacg acgagacgca gggcgcgatt ctgcccgccg gcgtgcaccc cctgggcgaa
     9601 ggtggctggg cagtcttcct gacctacacc gacgatggcc acatcgacga ttccgatgcg
     9661 caagaaatcg actacgacga catgctcaag gacatgaaag aaagcatcga agacagcaac
     9721 gaggcgcgta aacaggctgg ctatggggaa atgcacctgg tgaggtgggc cgagccgccg
     9781 cgttacgatg ccgcgggcaa gaaactctac tgggccaagg aaatatcggg tgcgagcggc
     9841 gagatgcatt cgctcaacta cgatgtgcga gtgctcggac gcgagggcgt gctgtccatg
     9901 gaagccgtcg cgcccatgga cctgctggct caggtccgca cagatatgcg tccgctgctc
     9961 gaagtcgccg aattcaacga aggctatcgc tacgccgagt tcaacgcgaa aaccgaccgc
    10021 gtggccgaat acgggctcgg cgcgctgatc gccggtgtcg ccgccaccaa gctcggcttg
    10081 ttcgccaagc taggggcact gctggccccc ttctggaagt tcatcatcat cggcttcgcc
    10141 gccctcgggg gattcctggc caagatattc ggcaagaaga aggacgaggc catctgagcg
    10201 aatcctcacc gtcagacttt cacgttcaca tcgagccggg cggccacgac attcgtgtgc
    10261 cgcccggcga gaacgtgctg accgcggcgc ttgccgccgg catcccgctt cccttcagct
    10321 gccgcgcggg ccgctgcgcc tcgtgcaagg caaagctcct caccggaagc atcgaatatc
    10381 ccacgggtct gccgcccggt atcaccaagg cggaggcgga gcgcggcgag gtgctgctct
    10441 gccaggcgcg gccgcgcagc gatctatcta tcgaatcgcg gcgcgtgccg gtgcgcgtgt
    10501 cgaacgcggc cagctgcgag atcgtggcga tcgagccgct ggcgctgcgg gccttgttgc
    10561 tgcgacttcg attcgtggag ggcaaggtcg acgcacatcc aggtcagttc gtggacgtgc
    10621 gcaatcacgc cggcgacgcg gaacgcctcg cggtcattgg cacaggaacg ggcgtggtcg
    10681 agctcgagtg tggagacgac ggcagtctgt tgcgcgcctg gttggactcg agcgccgttc
    10741 atggctcgat actgcgagtc gccggaccgt tcgaccggcc gcgttgagat tctctagatc
    10801 cggaagcggt ccttcagatc gcccgccatg cggcggctga cctcgagcgc gtccggcaag
    10861 ccgcgcagat gcacgaagtg ctggccgctc gcatcgcgcg caattcgctc caggtatttc
    10921 gtgttcacca gcgcgttgcg gtgaatgcgc acgaagttcg tcgcgaactc ttcctccagc
    10981 gttttcagcg agtcttcgat caggtcctca cccttcaggt gtttgaccgt ggtgtatttc
    11041 tggtcggcct ggaagcaaaa cacctcttcg atcggaatca gcctgagtcc atcccgtccg
    11101 cgaacaccga tgtgcgaacg atgcgctgcg gtccgcgtgg cggtcgccac ctgctgcagc
    11161 tgcgcgcgcg acaggcgttc ggccttggcg agcgcagcgc gcaacttctc ggcgcgaatc
    11221 ggtttcaaca ggtacgccac ggcttcggaa tcgaacgcct gcagcgcgta ctcgtcgaaa
    11281 gcagtggtga agatgaccgc cgggggcgcg ggcagcgtgg cgagatgtcg cgccacctcc
    11341 agcccgtcga tgccgggcat gcgtacgtcg agcagcacga tgtcgggcgt cagctggtcg
    11401 accatgtcga tcgcctgttc gccattgccg gcttcacctg ccaacacgaa atcgccggct
    11461 tccgcgagca tggagctcag acgctcgcgc gccggcggct cgtcatcgac gatgaggacg
    11521 cggaggctca tgcgtccgcg acggaggtcg tgaacgggaa acgcagcgtc acgatgtatt
    11581 cctcgtcgaa gcggccggcc ttgaccgtgg ctcggtcgcc gtacatcagg ccgagccgtt
    11641 ccttgatgtt ggccagcgcc aggcgattgc cgtcgcgtac cgtcaggctc gggagcggca
    11701 ccgggttgcg aacgacaatg gtgatcatgc ccttgttgaa ttcgcccgtg accgtgacca
    11761 cgccgccgcc cgggcgcggc tcgacgccgt ggtagatggc gttttcgagc aggggctgca
    11821 gcgtgagtcc ggggaccagt gcatcggtgg gcagcgagtc caccttccag tcgacgcgca
    11881 ggcgatcgcc gagacgcaat tgctccatgc gctggtaagt gcgcgccacg tcgatttcct
    11941 cggcgagcgg gatctggcca cgcttttcat tgagattggc gcggaacagc tccgccagat
    12001 cgagcacggc ttcctcggcg cgcgcggggt tgctgcgcgt gagcgcggcg atggtgttca
    12061 tgctgttgaa gaggaagtgc ggccggatgc gcgcctgcaa cgcgtgcacg cgggccttgg
    12121 cctgcagttc gacgttgtgg cgccattcgt gggtgacgta gaagtagcgc agcgccagcg
    12181 ccgtgaccac gaaagtgatg ccggcattac gcgcgaggaa ggaccagtgc tggtcctgca
    12241 gcaggccgaa gccgaagtag taggtcacgg cggacaccac cgtcacgagc gtcaccgtca
    12301 tggtgagcac catggcggca ctcaccgcga cgctctggcg gctcagatag tcacgcagca
    12361 ggcacagcag ggctgcgcca cacaacgcga tccacagcag gaacatggaa gtcaggccca
    12421 ggtcgctcca gaagccgagc gccgcttcgt ttcgcgccag cgcgagcaac aaggcggtga
    12481 gctcgcagat caggatgatg atcagcacgt tcgacgccga acagaaattg ggtagataga
    12541 gctggcgggg tccctcgccg ggtgctgccg gatactgcgg cgggtggttc atgccttgtg
    12601 cttcgcggca tcctcgaaca gcgcctgctt gagccggtgg gtcgcggaga tgtcttcgtc
    12661 gccgtaaccc tgctccatga gctcggcgta ttcggccagc atcgagtcga ccacgggcaa
    12721 tgacaccccg aaacgtgccg ccatgtcgcg gcagatgcgc agatccttgg cgtgcagctt
    12781 gatgcggaag ccggccggat aactgccacg aaccatgttt ggcgcgcgat gcacgaaata
    12841 ccagctcgag ccggcgccgg cgccgagcgt ggacaccacg cgatcgagcg gcaactggtg
    12901 cgcgcccgcg aacgccatgg cttcggcaca ggcgcggatg atgccggcgc acatgatctg
    12961 attggtggcc ttggtcgcct ggccggaacc cgcgggaccg aaatgttcga tggttttgcc
    13021 catcgccgcg aggatcgggc gcgcggcttc aaaagcagcg gggtcaccgc ccgtcatgat
    13081 ggcgagcgtg cctttctgcg cgccctcgac accaccgctg accggagcgt cgatgaactg
    13141 cgccgaccgt gcggagagaa actcttccgc ccagcgtgcc gtttctgcac tcacggtgga
    13201 gcagtcgatg acgagcgcac ccttgcgtag tccagggccc aggccctcta cgacggaacg
    13261 gacgtccgca tcggcggata cgcagaccac gaccacgtcg accatcgcgg cgagatcggc
    13321 aagtgttgcg ggagattgac agccaagctc cgcggcaagc gcgaccgctt tatcgctgct
    13381 gcgattccag acggcttcga gcagcccggc acgatggagg ttttttgcca tgggagcacc
    13441 catggcaccg agacccgcaa agccaactcg cttcataggc gcgaattatc aggtgcgagc
    13501 gccacaaagg aaaacgccgt ccagaggacg gcgttcagtg agcatgtgga cggaactcag
    13561 cgctcgtcaa ccctgcgggc cgcagatggc ttcgacggcc ctggcggcgt ccatgcgctg
    13621 ctgcgccagt tcggcttcgg acaggtattc acgttcgccg tttttgccgg tgcgatacat
    13681 gcgatgcgag ctgatggaac tgttgtaagt ctcgcgcgct ttcttgcaac gctcggcgcg
    13741 tgaagcctcg aggtccttgg cgacagtcgc ggcgacgcgc gaattgtcct gggagtcggt
    13801 gatgcgctga ttgctggcat tgagctggct gttggtcgca gcctggttcg cagcgtaatt
    13861 gcgttgggcg acttccggtg gccgttgcga accggtgctc accagtatgg cgcccggaat
    13921 gggcttgtcg gtgtagagcg tggtgcctcg ctcgtccacg tacttgtaga cgtctccagc
    13981 ctgtgccaat ccactattga agagggcgca agcaagaacg cctgtgatga cgacaagaat
    14041 cttcatatga cgcatggcct tcctccggta cgcggattat tgcaaagacg gcctataaga
    14101 acccttgatg tccatcacag acaagcccct ttccagaggc aaaaggggca aaagagaggg
    14161 cctcccgagg gaggccctga tttggacgaa atcccgggct ggccggggaa acccggccag
    14221 cccggggtga ggtcagccgt tgtaggcgcg ttcgccgtgg gtcacggtgt cgaggccttc
    14281 gcgttcctgt tcctcgttca cgcgcagacc gatcgtcaga tcgaccagct tgaacgcgat
    14341 cagggagacg aggcccgacc agagcagcgt cacgcccacg ccccagagct gcgatacgac
    14401 gtgtgcgccc atgtcgtagg ccgccggatc cgggtaggca ttggcaacgt agtcgtacac
    14461 gtcggtgcca cccagcgccg ggtcgttgaa cacgccggtc aggatggcgc cgacgatgcc
    14521 gccgacgcag tggacgccga acacgtcgag tgagtcgtcg tagcccagca tcttcttgag
    14581 tcccgagcag gcccacacgc agatcagagc ggcgacggcg ccgatggcga tggagcccat
    14641 cgttcccacc cagccgcagg ccggagtgat ggcgacgaga ccagcgaccg caccggatgc
    14701 cgcaccgagc atcgtgggtg aacccttgat gatccactcc gcgccgatcc atgcgagcgt
    14761 cgcagcggcg gtggcgagca gcgtgttggt gaacaccagc gtcgcgaagc cgttcgcttc
    14821 gaggttcgaa ccgacgttga agccgaacca gccaacccac agcagcgagg cgccgatcat
    14881 gaccatgggc aggttgtgag gcggcaatgc cgtcgttccg aagccgatgc gtttgccgag
    14941 cagcagcgcg cctaccaggc cggcgatacc ggcgttgatg tgcaccacgg ttccgccagc
    15001 aaagtcgagg gcgcccttgt tgaacaggaa gcctgcattc acgttggctg cggccccagc
    15061 ttcggcagtc gtgtacgcat ccggaccgga ccagaaccag accatgtggg cgatcgggta
    15121 atagctgaat gtcagccaga ggatgatgaa caccaggatc gcgctgaact tcacgcgttc
    15181 ggcgagggcg ccgacgatga gtcccggggt gatagccgcg aacgtcgcct ggaagacgaa
    15241 gaaccctagc tcgggaatgt tcgtggcctt gctgaaagtg ccggtgatcg aatcgccggt
    15301 catgcccttc aggaacattt tgctgaagcc gccgaagaag gcattgggac cttcggtgaa
    15361 cgccacgctg tagccgtaga tggcgaacat caggatgccc aggcagaaaa tgatcatcac
    15421 ctgcatgagc agcgacaaca cgttcttgct gcggaccatg ccgccgtaga acagcgccag
    15481 tcccggtatc gtcatcagaa tgacgatcac cgtgctcgtc atgagccagg agatgtcgcc
    15541 cttgttcggg ggcgggggcg cgtcctgccc gaataccagc gccggcaaca tcaacagcag
    15601 cgcgccgaga ccgcgcatca cgctgctacc cttcgttctc ttcttatctt gatgcatgta
    15661 aaagctcctc aacgacatct caggctgccg ccgcgccgga ttcacgggta cgtatccgca
    15721 ccgcttcacc gactggcgcc ag
//
LOCUS       DASQJS010000090        17423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1304558, whole genome shotgun sequence.
ACCESSION   DASQJS010000090 DASQJS010000000
VERSION     DASQJS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17423
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304558"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VEW08_03590"
     CDS             <1..184
                     /locus_tag="VEW08_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39847.1"
                     /translation="VVFVPFVNGKPSGMPLDVLTGFVSKDGVAWGRPVGVAIDKRGAL
                     LVADDVGNVVWRVTAR"
     gene            complement(171..500)
                     /locus_tag="VEW08_03595"
     CDS             complement(171..500)
                     /locus_tag="VEW08_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39848.1"
                     /translation="MAAAMWVRLGISGTELGMANLANASISGAFLETTLQLPVNANVI
                     LEPVSTAGVALEGVKLSARVARVDRRGLGIEWRAMIAPQLLALLTGTPLPSEGATATS
                     IGEITWP"
     gene            complement(594..2951)
                     /locus_tag="VEW08_03600"
     CDS             complement(594..2951)
                     /locus_tag="VEW08_03600"
                     /inference="COORDINATES: protein motif:HMM:NF003617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HYJ39849.1"
                     /translation="MPDQTLSPEQLRTVDAWWRAANYLSVGQIFLFDNALLKRPLVLA
                     DVKRMLLGHWGTTPGQNFIYAHLNRVISKYDLDMLYVSGPGHGGPAVVANTFLEGTYS
                     EVYPDITQDEAGLQKLFRQFSFPGGIPSHVSPECPGSIHEGGELGYSLSHSFGAAFDN
                     PDLIVACVVGDGEAETGPLATAWHSNKFLDPVTDGAVLPILHLNGYKIASPTLLARIT
                     REELEQLLRGYGWTPYFVSGHEPALMHDAMAGTLDVAIEQIRSIQRESRKHRNALRPR
                     WPMIVLESPKGWTGPKVVDGKPVEGTFRAHQVPLHPAEHPGHLQLLENWLRSYRPEEL
                     FDAGGRLKPEIAELSPRGARRMSANPHANGGQLLRELRLPDFRDYAIQVNVPGAAGGG
                     DTRALGPFLRDVATLNQELRNFRVFGPDETASNGLGAMFEMTDRQWDAEESTGDDHLA
                     RTGRVLDSMLSEHQCEGWLEGYLLTGRHGLFNCYEAFIHVIDSMFNQHAKWLKVTAGI
                     PWRHKIASLNYLLASHVWRQDHNGFSHQDPGFIDHVINKKADVVRVYLPPDANCLLSV
                     MDHCLRSRHYVNVVIAGKHPAPQWLPMPAAAEHCTAGIGIWQWASNDNRETPDVVMAC
                     CGDVPTLETLAAVSILREHLPEVKVRVINVVDLMKLQPKSAHPHGLSDLEFDALFTTA
                     TPVIFAFHGYPSLIHRLTYRRSNHDNIHVHGYKEEGTITTPFDMTVMNELDRFHLAIA
                     AIDRMPRTSDRGVELQHDLQRKLREHKRYIQAFGEDMPEIRNWQW"
     gene            complement(2960..4147)
                     /locus_tag="VEW08_03605"
     CDS             complement(2960..4147)
                     /locus_tag="VEW08_03605"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HYJ39850.1"
                     /translation="MSATHSILTINGGSSTLRFALFDASETPRLLLRGKIERIGFEGA
                     RWTVNRPDGTQEGRGIVAANHAAAATYLLNWLESEPAFSTVLAVAHRVVHGMAHSTPE
                     RVTRELLGDLRRIMAFDPEHLPFEIDLIAAIGQRHPALLQVACFDTAFHRTMPKVARS
                     LPIPRRYQSMGVERYGFHGLSCAFLMQELERLGDPAARSGLAILAHLGNGASLTAVRD
                     GRSVDTSMGFTPASGTMMSTRSGDLDPGLSHYLAQTQQMDTASFQHMVNHASGLLGVS
                     EISSDMRDLLALEKTDERAAEAIALYCYQAKKWIGALAAALGGVDTLVFAGGIGENAP
                     LIRERICSGLGFLDIELDGARNAESAALISATNARVRVRVIRTDEELVLARSALPFCS
                     RPE"
     gene            complement(4436..6088)
                     /gene="pgm"
                     /locus_tag="VEW08_03610"
     CDS             complement(4436..6088)
                     /gene="pgm"
                     /locus_tag="VEW08_03610"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HYJ39851.1"
                     /translation="MTLTPPINPLAGKLAPANMLVDLPKLITAYYSERPDPSVAAQRV
                     AFGTSGHRGVSFQASFNEWHVLAMTQAICTYRKQQGITGPLFLGFDTHALSAPAFASS
                     LEVLAANEVEVMIAKGDEYTPTPALSHAILTYNHGRTSALADGIVITPSHNPPDSGGF
                     KYNPPNGGPADTQITGWVQDEANRLLAASLSGVKRITLAKARAASTTHPHDYLHTYVR
                     DLANVIDMDVIRGANIRMGVDPLGGAGVHYWAAIADLYKLSLTVVNDAVDATFRFMTV
                     DWDGKIRMDPSSPHAMNGLIGLKDRFDIAFACDTDHDRHGIVTRSAGLLPPNHYLAVS
                     IDYLFRNRPGWRADAGIGKTVVSSQIIDHVAARLGRRLFEVPVGFKWFVDGLFDGSLG
                     FVGEESAGASFLRRDGKVWTTDKDGIAPALLSAEMTARTGRDPGEAYRELTRAFGEPL
                     FERIDAAATPAQKKLLANLKPEQIKSKELAGGKIQSILTSAPGNGAGIGGLKVVADGG
                     WFAARPSGTEDVYKIYAESYRNGEHLRAVQAEAQAIVTEAIA"
     gene            complement(6159..8054)
                     /locus_tag="VEW08_03615"
     CDS             complement(6159..8054)
                     /locus_tag="VEW08_03615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ39852.1"
                     /translation="MVTEQVLAESERRFRALVTASSDVVYRMSADFGELRQLEGRLFI
                     TASPVPNRNWLDEYIHPDDRELARDAIRDAIGRKGMLELECRVFLADGSVGWTHTRAV
                     PMLDASGEITEWIGMVTNVTARKSAEHALANQRRMYEAILTNTPDLAYVWNREHRFIY
                     ANEGLLHMWGRTWEEAIGKNCLELGYEPWHAAMHDNEIEQVIATKQPVRGEVPFNGAF
                     GRRFYDYLLVPVIGASGEVEAVAGTTRDVTERKQLERSLIDADRRKDEFLATLAHELR
                     NPLAPIRNAVHLLKADNLTEDNVRAARQIIDRQVHHMVRLVDDLMDVSRITLGQVNLR
                     YDRVSLRRVIDDALEAAAPAIEAGNHRLVVDVPGASLQLEGDATRLSQVFQNLLDNAA
                     KYTPPGGTITLRAELRDQQIVVSVRDTGIGISPEAKARVFELFTRVHPADRIKTSGLG
                     IGLSLAKKLIELHLGEIEVRSEGTGTGSEFVVTLPAPVATAAPVPAVDTVISLAKPDG
                     SQRVLVVDDNRDAAESLGMLLEMEHCKVSVAFDGLHALEALDTFKPDVALLDIGMPGM
                     DGYELARRIRATPRGRNLVLVALTGWSQADDKQRASDAGFDEHLTKPVDPEVLSRVLN
                     RKRSAAA"
     gene            8283..9758
                     /locus_tag="VEW08_03620"
     CDS             8283..9758
                     /locus_tag="VEW08_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HYJ39853.1"
                     /translation="MDTRRKWKCVQGTVAAAAFAAAVSISGGSFAAEGGGSSYLQGTY
                     GDFAAAVTRAPGGYLRNDVIYHDAGIGARPINGGLDPGFDQKLWMDRLSFAGFADGGF
                     FGGKFGIEVQIPYVADLTVAQRPGGPPFDSFGEPRDHAIGDPVIKPQLAWGSGPHYSR
                     LSLGIVAPWGTADDSRVVSVGRHYWSFDPAFTYTYLSDNGWDLSWTLGYMVNLENSLP
                     GPNYETGDELHFDALFGKHFGDHFALGIAGYWYSQMTDDKGPIPAPLESGYRSEGAGF
                     GPAFMFGTKNFSVVGKWLTDEQAENRLDGDLYMLSIVARFGGESPPPPMPVAPPVARV
                     EPPPAPPPPPPADADGDGVVDQSDLCPATPRGDRVDVNGCACDVTVQVNFRSNSTQIY
                     GADAITLDSLADQVRRLPTIVGELGGHTDSQGAEAFNVDLSRRRALAARDYLASRGLD
                     VTQLAIAGYGESQPIADNATAEGRALNRRVVMRRTNCQVGP"
     gene            complement(9819..10745)
                     /gene="ppk2"
                     /locus_tag="VEW08_03625"
     CDS             complement(9819..10745)
                     /gene="ppk2"
                     /locus_tag="VEW08_03625"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HYJ39854.1"
                     /translation="MSAQPPIDHEQLIQRIRRDLLDSWDEEIEMDVEDYHPLPELPDG
                     GANGAGEKDYRRNYFQQLFRLQAELVKLQDWVYASKQKVVILFEGRDAAGKGGAIKRI
                     TQKLNPRVCRVVALPAPNDRERTQWYFQRYVSHLPAAGEIVLFDRSWYNRAGVEHVMG
                     FCSDEEYEDFFRSVPEFERMLVRSGIRLLKYWFSITDEEQYLRFLGRIHDPLKQWKLS
                     PMDLESRRRWEDYTKAKEIMLERSHIPEARWWVVQAVDKKRARLNCIDHLLRQFPYAE
                     VPKQGVVLPERERHEHYSRQAVPAEMLVPEIY"
     gene            complement(10851..11354)
                     /locus_tag="VEW08_03630"
     CDS             complement(10851..11354)
                     /locus_tag="VEW08_03630"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYJ39855.1"
                     /translation="MTEAEYKLESRLWKSLKDDRTVMLGLAGVEEGHSQPMSAQILHD
                     EDERGPIWFFTSTDSDLVRALTDTHRAVVHFSSKGHDLFASLHGALTLDNNRQIIDQL
                     WNSFIAAWYEGGKEDPKLQLLRLDPERAQVWLNENNLWAAVKLLLGSDPKKDYKDKVG
                     DVRLGTH"
     gene            complement(11541..11879)
                     /locus_tag="VEW08_03635"
     CDS             complement(11541..11879)
                     /locus_tag="VEW08_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HYJ39856.1"
                     /translation="MKSIKYLLPLVALAASGIAAASAAREAPGVVVRYGDLDLNSQAG
                     VASLHKRIRNAAASVCSQLETRILGLHDAYDRCVKEALSNGVAAVANPNLSNFHASKG
                     KGAVVASIRQ"
     gene            complement(12008..12976)
                     /locus_tag="VEW08_03640"
     CDS             complement(12008..12976)
                     /locus_tag="VEW08_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYJ39857.1"
                     /translation="MDALSDVLRAVRLTGAFFFDVHACAPWCAETPFGKTVVDAMFPG
                     SDHLICYHLLMEGSCWATLEGEEPIKLVAGDIIVLPQGDTHVLATTPGMRKSPEMSMY
                     RRPNDGKLPSKISMGSDGGEAAHFVCGFLGCDSRPYNPLLTALPRVIVIHDHGSGALG
                     AYFRAALAESKSERMGSADLLGRISELMFVDVVRQYLESQPADRTNWLSGLRDQYVGR
                     ALMALHADPARDWTLEALAHEAALSRSSFAERFTQFVGHPPMQYLTNWRMQLATNFLR
                     NGTENVAAIANRVGYESEAAFSRAFKKVVGAPPSEWREQRLTGNAA"
     gene            13110..13706
                     /locus_tag="VEW08_03645"
     CDS             13110..13706
                     /locus_tag="VEW08_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HYJ39858.1"
                     /translation="MMKAFCAVTAMTIAGSSAVATAAEPSQATHRYLIERTFPPGALD
                     GVDAAAKKKVNENNATLDVTWEKSYANADKTKLYCVYDGPNENAVREAAKLNGLPVDN
                     VTEIPADIKAEPRGAVHHIASGNQRYLVKRSGAPVVTASSDKKFGVTLITSYATADKR
                     DTFWVYEAPSYSAVESAAKASGAPFESIAEIPETLYPN"
     gene            complement(13779..14300)
                     /locus_tag="VEW08_03650"
     CDS             complement(13779..14300)
                     /locus_tag="VEW08_03650"
                     /inference="COORDINATES: protein motif:HMM:NF040422.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HYJ39859.1"
                     /translation="MRTGLIVFALATVAFTSLALAEKPLVKPYAAGAPYGGIPIWSPG
                     EKFVAVSSGTCDGNCPVYELYVFDDGRVVFNGRKGTSKVGVWNKKVEPEVYAELLTTL
                     VRTKVLDEEIKRKTCLKGRSVLTVMRSATDAGDVRTVLLNSGCEGYADLVREIEGQFI
                     SSTGVDRWLAPPK"
     gene            complement(14312..15703)
                     /locus_tag="VEW08_03655"
     CDS             complement(14312..15703)
                     /locus_tag="VEW08_03655"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HYJ39860.1"
                     /translation="MALSVFDLFKIGIGPSSSHTVGPMRAARQFAARLRDSQQLTRVT
                     RISVTLYGSLGATGKGHGTDKAILLGLSGHEPDTVDVEAIAGIVESIRTSGQLQLYGG
                     HAVAFDERRDLTFNHKDTLPLHSNGMLFAAYAEDGTGLSTGTYYSVGGGFVVSESETP
                     DGGTARRIVPDATVLPFPFRNGDELLALCEKENATIAAIMRRNERHWRTDAEIDQGLL
                     HIWHVMQACVKRGCETTGVLPGGYKVKRRAPGLHAQLTAPQMAAPHDPLIALDWVNLW
                     ALAVNEENAAGGRVVTAPTNGAAGIVPALMHYYARFVHGANDAGIVDFLLTAAAIGFL
                     YKENASISGAEVGCQGEVGVACSMSAAALTAVLGGSVRQVENAAEIAMEHHLGLTCDP
                     VGGLVQIPCIERNAIGAAKAINVSRMALRGDGTHYVSLDKVIKTMRETGADMKTKYKE
                     TSRGGLAVNIVEC"
     gene            complement(15719..>17423)
                     /locus_tag="VEW08_03660"
     CDS             complement(15719..>17423)
                     /locus_tag="VEW08_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYJ39861.1"
                     /translation="THPDDVEATRERVKKLRAGEIDNYALEKRYVRKDGSAVWSSTTV
                     TLLRKESGEAAQYIGIIQDITERKQTENVLARLAAVVEHTDDAIVTKTLDGIITTWNP
                     GAERIFGYTADEAIGKPVTILIPEKQLNEEPGILERLRRGERIDHYQTTRRRKDGTLI
                     DVSLTVSPIKDRSGRVVGASKIARDITHQKRAEATLREMDQRKDEFLATLAHELRNPL
                     APIRQATLISMSPSASEAQKRWSHEVITRQVHHMSLLLDDLLDISRITRGTLELRTVM
                     TNLTAVVDAAVETSRPALEAKHHEFLVELPPAPVQFAADPLRLAQVLSNLLTNAAKYT
                     DPRGTIRLRATADAKYVEFSVTDTGVGLPQDALSAVFSMFSQVKSSQDRSEGGLGIGL
                     ALSRGVVELHGGTIEARSAGLGKGSEFFVRIPRRKVSSHTQSTAGGAAVATPGKRRVL
                     IADDNRDAAESLAMLLEIEGHSVTVVHDGQQALAAIEAARPDVALLDIGMPEMDGYEV
                     ARQVRGDSRSRSTLLIALTGWGQEQDKARAMGAGFDMHFTKPVEPQRLIELLGAELPA
                     R"
BASE COUNT         3004 a         5497 c         5583 g         3339 t
ORIGIN      
        1 agtggtgttc gttccgttcg tcaacggcaa gccatccggc atgccgctcg acgtgctcac
       61 gggattcgtg agcaaggacg gcgtggcatg gggacggccc gtgggtgtgg ccatcgataa
      121 gcgcggtgcg ctgctcgtgg ccgatgacgt cggcaacgtc gtctggcgcg tcacggccag
      181 gtaatctccc cgatcgaggt agcggtcgcg ccttccgatg gcaagggggt ccccgtgagc
      241 agggcaagaa gctgcggcgc aatcatggcg cgccactcga ttcccaaacc gcggcgatcg
      301 acccgcgcga cccgcgcgga aagcttcacg ccttcgaggg ccacgccggc cgtcgaaacg
      361 ggctcgagaa tgacgttggc attgacaggt agttggagcg tcgtctcgag gaacgccccg
      421 ctgatgctcg cgttggcgag gttggccatg ccaagttcgg taccgctgat gcccaggcga
      481 acccacatgg cagccgccat tcgttgacct cggcggtgtt ccttgggttc tgccatggcg
      541 cgcaggttag acggttcggg gcgcggtgcg aatcagggaa attccgcagg ccatcaccat
      601 tgccaattgc ggatctccgg catgtcttcg ccgaacgcct ggatataacg cttgtgctcg
      661 cgcagctttc tctgcaggtc gtgctgcagc tcgacgccgc ggtcgctggt cctcggcatc
      721 cggtcgatcg ccgcgatggc caggtggaat cggtccagct cgttcatcac cgtcatgtcg
      781 aacggcgtgg tgatggtgcc ttcttccttg taaccgtgga cgtggatgtt gtcgtggttg
      841 ctgcgccggt aggtgagccg gtgaatcagg gacggatatc catggaacgc gaaaatgacc
      901 ggcgtggcag tcgtgaagag tgcgtcgaac tccaggtcgc tcaaaccgtg gggatgcgcc
      961 gacttcggct gcagcttcat gaggtcgacg acgttgatga cgcgcacttt cacctcgggc
     1021 aggtgttcgc gcaggatcga gacggcggcg agcgtttcca gcgtgggcac gtccccacag
     1081 caggccatca ccacatcggg cgtctcgcgg ttgtcgttgc tggcccactg ccagatgccg
     1141 atgccggcgg tgcagtgctc ggcggccgcg ggcatgggca accactgtgg cgccggatgc
     1201 ttgcccgcta tcacgacgtt gacgtagtgg cggctgcgca ggcaatgatc catgaccgac
     1261 aacaggcagt tcgcatccgg tggaagatag acgcgcacca cgtccgcctt cttgttgatg
     1321 acgtgatcga tgaagccagg atcctggtgc gagaaaccgt tgtgatcctg tcgccagaca
     1381 tgcgaagcca gcaggtaatt gagcgaggcg atcttgtggc gccacggaat gcccgcggtc
     1441 accttgagcc acttggcgtg ctggttgaac atcgaatcga tgacgtggat gaaggcctcg
     1501 tagcaattga aaagcccatg gcgccccgtc agtagatagc cttcgagcca tccctcgcac
     1561 tggtgctcgc tcaacatcga gtcgaggacc cgccccgtgc gtgcaaggtg atcgtcgccc
     1621 gtgctttctt cggcgtccca ctgccgatcg gtcatttcaa acatcgcgcc gagaccattg
     1681 gaggccgttt cgtcgggacc gaacacgcgg aaattgcgca gctcctggtt caacgtcgcc
     1741 acatcgcgca aaaatggtcc gagggcgcgc gtatcgccgc cgccagcagc cccgggaaca
     1801 ttcacctgga tggcgtagtc gcgaaagtcc ggcaggcgca gttcgcgcaa cagctggccg
     1861 ccgttggcgt gggggttcgc gctcatccgc cgcgcaccgc gcggtgacag ttcggcgatc
     1921 tcaggtttca gccggccacc cgcatcgaac agttcttcag gccggtaact gcgcagccag
     1981 ttttcgagca gctgcagatg cccgggatgc tcggcgggat gcagtggcac ctggtgcgcg
     2041 cgaaacgttc cttcgaccgg cttgccgtcg acgactttcg gtcctgtcca gcccttgggc
     2101 gattcgagca cgatcatcgg ccagcgcggc cgcaatgcat tcctgtgctt gcgcgactcc
     2161 cgctggatac tccgtatctg ctcgatcgcc acatcgagag tgcccgccat ggcgtcgtgc
     2221 atcagcgccg gctcgtggcc ggacacgaag tagggtgtcc agccataacc acgcaacagc
     2281 tgctccagct cttcgcgtgt gatgcgggct agcaaggtgg gactggcgat cttgtagccg
     2341 ttcaaatgca ggattggcag caccgcgccg tcggtgacgg ggtcgaggaa cttgttggag
     2401 tgccaggcag tcgccagcgg ccctgtttcg gcctcgccat cgccaacgac acaggcgacg
     2461 atgagatcgg ggttgtcgaa tgccgcgccg aaggaatggc tcagtgaata accgagctcg
     2521 ccgccttcgt gaatcgaccc cgggcactcg ggcgatacgt ggctcggaat gccacccgga
     2581 aacgaaaact gcctgaacag cttctgcagc ccggcttcgt cctgggtgat gtctggataa
     2641 acctcgctgt aggttccctc caggaaggta ttcgccacca ccgccggccc gccgtgcccc
     2701 ggacccgaca catacagcat gtcgaggtcg tacttgctga tcacgcggtt gagatgcgcg
     2761 tagatgaagt tctgtcccgg cgtcgtgccc cagtggccga gcaacatgcg tttcacgtcg
     2821 gcgaggacca gcggccgttt caacagcgcg ttgtcgaaca ggaatatctg acccacggac
     2881 aggtagttag ccgcacgcca ccaggcgtct accgtgcgca gctgttcggg tgagagtgtc
     2941 tggtctggca tgtttgtatt cactccgggc gtgagcagaa aggcagtgcc gatcgggcca
     3001 gcaccaactc ttcgtccgtg cggatgacgc ggaccctgac gcgagcgttg gttgctgaaa
     3061 tcagcgccgc ggattcggca ttccgcgcgc cgtccagttc gatatcgaga aaccccaggc
     3121 ccgagcagat gcgctcgcgg atgagcggcg cgttttctcc gatgcctccg gcgaacacca
     3181 gtgtgtcgac tccgcccagc gcagcggcga gcgcgccgat ccacttcttc gcctggtagc
     3241 agtagagcgc gatcgcttcg gcggcgcgtt cgtcggtttt ctcgagcgca agaaggtcgc
     3301 gcatgtccga gctgatctcg gaaacgccta gcaatccgga cgcatggttg accatgtgtt
     3361 gaaaactcgc cgtgtccatc tgctgcgttt gcgcgaggta gtgactcagg cccggatcga
     3421 ggtcgccgga gcgcgtgctc atcatggttc cagacgcggg cgtgaaaccc atgctggtat
     3481 cgacgctgcg cccgtcgcgc acggcggtca ggctcgcgcc gttgccgaga tgtgcgagga
     3541 tcgcgagtcc gctcctggca gccggatcac cgaggcgctc cagctcctgc atgagaaacg
     3601 cgcacgagag cccgtggaaa ccatatctct cgacgcccat cgactgatac cgccggggaa
     3661 tgggcaggct tcgcgccacc ttcggcatgg tgcgatggaa cgcggtatcg aaacaggcga
     3721 cctgaagcaa ggccggatgc cgttgcccga tggcggcgat cagatcgatt tcgaaaggca
     3781 agtgctccgg atcgaatgcc atgatgcgcc gcaggtcgcc cagcagctcg cgcgttacgc
     3841 gctcaggcgt ggagtgcgcc atgccatgca ccacgcggtg tgccacggca aggacggtgg
     3901 agaacgcggg ctcggactcg agccaattca atagataggt cgccgccgcg gcgtgattcg
     3961 ctgcgacaat gcctcgtcct tcctgcgtcc cgtccggtct gttgaccgtc cagcgcgcac
     4021 cttcgaatcc gatgcgctcg atcttcccgc gcaacagcag gcgcggcgtt tcactggcat
     4081 cgaacaatgc gaaacgcagc gtcgaggagc caccgttgat ggtcaggatc gaatgcgtcg
     4141 cgctcattca gtcagatacg ccagacagat gtaccacata tgcccgacct cgaataaact
     4201 catgcgatcg tcaacgtatc cctgaaaacc ttcgttgtcg acccttaagg tgatgacgac
     4261 gccggatgtc cagttgtgct gacagaacgg cggaaccgca ctatctacag tgctcatccc
     4321 cggatctgcc gtgtcgggtt actgaccatc atttctgttg agccacagca gtgcgcgccc
     4381 ggcgagcatt tcgcgtgctc tggagcgacg attcggtcgg gcggtccgat gattgctagg
     4441 cgatcgcctc cgtcacaatg gcctgcgcct ccgcctgcac tgcgcgaagg tgctccccat
     4501 ttcgatagct ctcggcgtag atcttgtaga catcttcggt gcccgacggc cgcgcggcaa
     4561 accacccgcc gtccgcaacc accttgaggc cgccgatgcc ggcgccattg cccggcgcgc
     4621 tggtgagaat ggactgaatt tttcccccgg ccagttcctt cgatttgatc tgctcgggct
     4681 tgaggttcgc cagcaacttt ttctgggccg gcgtcgcggc cgcgtcgata cgctcgaaca
     4741 ggggctcgcc aaaggcgcgc gtaagctcgc gatacgcctc gcctggatcg cgcccggtgc
     4801 gcgccgtcat ttccgccgag agcaatgcag gcgcgatgcc atctttgtcg gtagtccaca
     4861 ccttgccgtc gcggcgcaga aacgacgcac ccgcgctttc ctcgccgacg aatccgagtg
     4921 acccgtcgaa caatccgtcc acgaaccact tgaagcccac cggcacttcg aaaagtctgc
     4981 gcccgagcct ggctgccacg tgatcgatga tctggctgct gacgacggtc ttgccgatgc
     5041 ccgcatccgc gcgccagccc ggccgattcc ggaacaggta gtcgatggac accgccaggt
     5101 agtggttcgg cggcagcagg ccggcgctgc gcgtgacgat gccgtgccgg tcgtggtcgg
     5161 tgtcgcaggc gaacgcgatg tcgaagcgat ccttgaggcc gatgaggccg ttcatggcat
     5221 gcggcgaaga tggatccatg cggatcttgc cgtcccagtc cacggtcatg aagcggaagg
     5281 tggcgtccac cgcgtcgttg accaccgtga ggctcagctt gtagagatcc gcgatcgccg
     5341 cccagtaatg cacgccggcg ccgccgagcg gatcgacacc catgcggatg ttcgcgccac
     5401 ggatcacgtc catgtcgatg acattggcca gatcgcgcac ataagtgtgt agatagtcgt
     5461 gcgggtgcgt ggtagacgcg gcgcgcgcct tcgcgagcgt gatccgtttc acgccactca
     5521 gggaagcggc cagcagccgg ttcgcttcgt cctggaccca gccggtgatc tgcgtgtccg
     5581 cggggccgcc gttcggcggg ttgtacttga agccgccgct gtccggcggg ttgtgcgagg
     5641 gcgtgatcac gatgccgtcc gccagggcgc tcgtgcgccc atggttgtag gtcaggattg
     5701 cgtgcgacag cgcgggcgtc ggcgtgtact cgtcgccctt ggcgatcatg acctcgactt
     5761 cgttggccgc caggacttcc agcgagctcg cgaacgccgg cgcggacagt gcgtgcgtgt
     5821 cgaagccgag aaacagcggg cctgtgatgc cctgttgctt ccggtaagtg cagatcgcct
     5881 gcgtcatggc gagcacatgc cattcgttga agctcgcctg gaaggaaacc ccgcggtgcc
     5941 ccgaggtgcc gaacgccaca cgctgcgcgg ccaccgatgg atcaggacgc tcgctgtaat
     6001 acgccgttat gagtttaggc agatcgacca gcatgtttgc cggggcgagc ttgcccgcga
     6061 gtgggttgat tggcggcgtc agcgtcatct cgggtcttcg cttggttggt ggatgattcc
     6121 acgaatcatg cactctccgc gactacttga gcgcgctttc aggcggcggc ggatcgcttg
     6181 cgattcaaca cccggctcag cacctccgga tccacgggct tggtgaggtg ctcatcgaat
     6241 ccggcatcgg acgcgcgctg cttgtcatct gcctggctcc agcccgtcaa tgccaccagc
     6301 accaggttgc ggccacgcgg cgtggcgcgt atccgccgtg cgagctcata accgtccatg
     6361 cccggcatgc cgatgtcgag caacgcaacg tcgggtttga acgtgtccag tgcttcgagc
     6421 gcgtgcaagc cgtcaaatgc caccgaaacc ttgcaatgct ccatttcgag cagcatcccc
     6481 aggctttcgg cggcgtcgcg gttgtcatcg accaccagca cgcgctggct gccatcgggc
     6541 ttcgcaagag atatcacggt gtcgactgca ggaaccggcg ccgccgtcgc gactggcgcc
     6601 ggcaaggtca ccacgaactc gctgcccgtg ccggtgcctt cgctgcggac ttcgatttcg
     6661 cccaggtgaa gttcgatgag cttcttcgcc agggacaagc cgatgccgag accgctggtc
     6721 ttgatccggt cggcgggatg cacacgcgtg aacaactcga acacgcgcgc cttcgcttcc
     6781 ggcgaaatgc cgataccggt gtcgcgcacc gacaccacga tctgctgatc acgcaattcg
     6841 gcgcgcaacg taatcgtgcc cccgggaggc gtgtacttgg cggcgttgtc gagcaggttc
     6901 tgaaagacct gcgacaggcg cgtggcatcg ccttcgagct gcagggaggc acccggaaca
     6961 tcgacgacca accggtggtt gccggcttcg atcgccggtg cggcggcctc gagcgcatca
     7021 tcgatcacgc ggcgcaggct gacgcggtca taacgcaggt tcacctggcc gagcgtgatt
     7081 cgcgaaacgt ccatcagatc gtcgaccagg cgcaccatgt gatgtacctg ccggtcgatg
     7141 atctggcgcg cggcgcgcac attgtcttcg gtgagattgt ccgctttcag caggtgcacc
     7201 gcgttgcgga tgggggcgag cggattgcgc agctcgtgcg ccagggtcgc caggaattca
     7261 tccttgcgcc ggtcggcgtc gatcagcgag cgttcgagct gtttgcgctc ggtgacgtcg
     7321 cgcgtcgtgc cagccacggc ctcgacttct ccggaggcgc cgatgaccgg caccagcaga
     7381 tagtcgtaga aacgccggcc gaaagcgcca ttgaaaggca cttcgccgcg caccggctgc
     7441 ttcgtcgcga tgacctgctc gatctcgttg tcgtgcatgg cggcgtgcca gggttcataa
     7501 cccagctcga ggcagttctt gccgatggct tcttcccacg tgcggcccca catgtgcagc
     7561 aggccttcgt tcgcgtagat gaagcgatgc tcgcgattcc agacgtaggc gaggtcgggc
     7621 gtgttggtga gaatggcttc atacatgcgc cgctggttgg cgagcgcgtg ttcggcgctc
     7681 ttgcgcgccg tcacgttggt caccatgcct atccactcgg tgatctcgcc gctggcgtcg
     7741 agcatcggta ccgcgcgtgt atgcgtccat cccacgctgc cgtcggccag gaatacgcgg
     7801 cactcgagtt cgagcatgcc cttgcggccg atggcgtcgc ggatggcgtc ccgcgccagc
     7861 tcacggtcgt caggatggat gtattcatcg agccaattgc ggttggggac cggcgaggcg
     7921 gtgatgaaca gccgtccctc gagctgccga agctcgccga agtcggcgct cattcgatag
     7981 acgacgtccg agctggccgt gacgagcgca cgaaatcgcc tttcactttc cgccagtacc
     8041 tgctccgtga ccatcgaact gttcctgcca ttcctgcgtg ggagcagagg atgcaacacg
     8101 cgcggggcgc gtgggaaatg gtaggaagcc tcctacagca ttcttccggt ttctccattg
     8161 tcgccagtgc gcgacgaatg cggcgccacc cgatgcgatt gcccggccgc gggactaggg
     8221 gagtagttag gcgggccctt ggtcctattc cacgtgttgc cggagcgtgc gagcatcggc
     8281 acatggacac gcggcgcaaa tggaagtgcg tgcagggtac ggtagccgcg gcggcgttcg
     8341 ccgccgccgt ctcgatatcc ggcggctcgt ttgccgccga gggcggcggc agctcctatc
     8401 tacagggaac gtacggcgat tttgccgccg cggtcacccg tgcgccgggc ggctacctgc
     8461 gcaacgacgt catctaccat gacgcgggca tcggtgcccg cccgatcaac ggcggcctcg
     8521 atccaggctt cgatcagaag ctgtggatgg atcgcctgtc gtttgcgggg ttcgcggacg
     8581 gtggattctt cggtgggaaa ttcggcatcg aggtgcagat tccttacgtg gccgatctga
     8641 ccgtcgccca gcgcccgggc ggaccgccgt tcgattcctt cggagagccg cgcgaccatg
     8701 cgataggtga tccggtcatc aagccgcagc tcgcgtgggg ttccggtccg cactacagca
     8761 ggctctcgct cggcatcgtg gcgccgtggg gcaccgccga tgacagccgc gtggtctcgg
     8821 tcggccggca ctactggtcg ttcgatccgg cgttcaccta tacctacctg agtgacaacg
     8881 gctgggattt gtcctggacc ctggggtaca tggtcaacct cgagaacagc cttccgggtc
     8941 ctaactacga gactggcgac gagctgcact tcgacgcgtt gttcggcaaa catttcggcg
     9001 accacttcgc gctcggtatc gccgggtact ggtacagcca gatgaccgat gacaaggggc
     9061 cgattccagc gccgctcgag tccggttaca ggagcgaagg cgcgggcttc gggccggcgt
     9121 tcatgttcgg cacgaagaat ttcagcgtgg ttggcaaatg gctgactgac gaacaggccg
     9181 agaaccgtct cgacggagac ctgtacatgc tgtcgatcgt ggcgagattc ggcggcgaat
     9241 ctccgccgcc gccgatgcca gtagctccgc cggttgcgcg cgtcgagcca ccgccggcac
     9301 cgccgcctcc gccaccggcg gatgccgatg gcgacggcgt ggtcgaccag tcggatctgt
     9361 gtcccgcgac gccgcgcggc gatcgcgtgg acgtcaacgg ctgcgcctgc gatgtcacgg
     9421 tgcaagtgaa cttcaggagc aactccacgc aaatctacgg cgcggatgcg atcacgctcg
     9481 acagcctcgc agaccaggta cgccgcctgc cgacaatcgt cggggaactc ggcggacata
     9541 ccgacagcca gggcgccgaa gcattcaacg tcgacctgtc gcgacggcgt gcgctggctg
     9601 ccagggacta cctggcgtcg cgcgggctcg atgtgacgca gctggcgatt gccggctacg
     9661 gcgaatcaca accgattgcc gacaacgcca cggcggaagg gcgtgcgctg aaccgccgcg
     9721 tggtcatgcg ccggacgaat tgccaggtgg gcccataaga ggtgccgggt ggggaaagtg
     9781 gggagtgctg cactccccac tttccccacc cggcaccgtc agtagatctc cgggaccagc
     9841 atttccgccg gtactgcctg gcgcgaatag tgttcgtggc gctcgcgttc cggcagcacc
     9901 actccctgct tcggcacctc ggcgtaggga aactgcctga gcaggtgatc gatgcagttg
     9961 agccgcgcgc gcttcttgtc cacggcctga accacccacc agcgcgcctc gggaatgtgg
    10021 ctgcgctcga gcatgatttc ctttgccttg gtgtagtcct cccagcggcg gcgcgactcg
    10081 agatccatgg gcgacagctt ccactgcttc agtggatcgt ggatgcgccc gaggaaccgc
    10141 aggtattgtt cctcatcggt gatcgagaac cagtacttga gcaggcgtat gccggaccga
    10201 accagcatgc gctcgaactc cggcaccgaa cgaaagaagt cttcgtactc ctcgtcgctg
    10261 cagaaaccca tcacgtgttc gaccccggcg cggttgtacc agctgcggtc gaacagcacg
    10321 atttcgccgg cggccggcag gtgtgagacg tagcgctgga agtaccactg tgtgcgttcg
    10381 cgatcgttgg gagcgggcag ggcgaccacg cggcagacgc gcgggttcag tttctgcgtg
    10441 atgcgcttga tggcgccgcc cttgccggcc gcgtcgcggc cctcgaacag gatgaccacc
    10501 ttctgcttgc tggcgtacac ccagtcctgc agcttgacga gctcggcctg cagtcggaac
    10561 agctgctgga aatagttgcg gcgatagtcc ttttcacccg cgccattggc gccgccatcc
    10621 ggaagctcgg gcagaggatg gtagtcctcc acgtccatct cgatttcctc gtcccagctg
    10681 tcgaggaggt cgcggcggat acgctggatg agttgctcgt ggtcgatggg aggctgggcg
    10741 ctcatgcggg ctccgcttgt taggacccgc gatgctcgcc cgcccgtatt acagtcgtgt
    10801 gaaaacggtg ccaggcaccg tattcaagaa aacggtgcct ggcaccgttt tcagtgcgtg
    10861 cccaggcgca cgtcacccac cttgtccttg tagtctttct tcgggtcgct tcccaagagc
    10921 agcttcacgg cggcccacag gttgttctcg ttgagccaga cctgcgcgcg ttcgggatcg
    10981 agtctcagca actgcagctt cgggtcttcc ttgccacctt cgtaccaggc ggcaatgaaa
    11041 ctgttccata actggtcgat gatctggcgg ttgttgtcga gcgtgagagc gccgtgcaac
    11101 gacgcgaaca ggtcgtggcc cttggacgaa aaatgcacca ccgcgcgatg cgtgtctgtc
    11161 agcgccctga ccaggtcgga gtccgtggac gtgaagaacc agataggacc gcgctcgtcc
    11221 tcgtcgtgaa gtatctgggc ggacatcggt tggctgtggc cctcctcgac gccggcgagt
    11281 ccgagcatca cggtgcggtc gtccttgagt gacttccaca acctggattc cagcttgtat
    11341 tcggcttcgg tcatgagatg cctccggtgt tggtcaccgg aggatcacat ccacgccagt
    11401 tggtgtgacg tggccgattc aggcgatcgg tgtcagcgcc tgcctgcacg gctgcgaaag
    11461 ctgattccgc gcgagcttcc gaattaatgc ggcgcgcgcg gcgttgcagg ctttgaaaac
    11521 ggtgcctggc accgttttca tcactgacga atagacgcga cgaccgcgcc ctttcctttg
    11581 ctcgcgtgga aattcgagag gttcggattg gccaccgcgg ccacgccgtt gctcaaggct
    11641 tccttcacac acctgtcgta tgcgtcgtgc aggccgagga tgcgtgtttc gagctggctg
    11701 caaacggacg ccgccgcgtt gcgaatccgc ttgtgcaggc tggcgacgcc agcctgggaa
    11761 ttcaggtcga gatcgccgta gcgtacgacg acgccggggg cttcgcgcgc agcggaggcg
    11821 gcggcaatgc cggaggcggc caacgctacg aggggtaaca gatacttgat cgacttcatg
    11881 atgtgctcct tggtagaggc gcacatgctg ccgatttgaa aaccgggcgt gttcctaaga
    11941 tctgacccga tgataagttt tactgaacga tggccggaaa ccgaatcagc ggttcacgct
    12001 actcacacta cgcggcgttg ccggtcaggc gttgttcgcg ccattcactc ggcggcgcac
    12061 ccaccacttt cttgaatgcg cggctgaagg ccgcctcgga ttcataaccc acgcggttgg
    12121 cgatcgccgc gacattttcc gtgccgttgc gcaggaagtt ggtcgcgagc tgcatgcgcc
    12181 agttggtgag gtattgcatg ggcgggtgcc ccacgaattg cgtgaagcgc tccgcaaacg
    12241 acgaacgcga caacgcggct tcatgcgcca gtgcttccag tgtccagtcg cgcgccggat
    12301 cggcgtgcag cgccatcaac gcacggccga cgtattgatc acgcaggccc gagagccagt
    12361 tggtgcgatc ggcgggttgt gactcgagat attgccgcac tacatcgacg aacatgagct
    12421 cgctgatgcg gcccaggaga tccgcgctgc ccatgcgttc gcttttcgac tcggccagcg
    12481 cggcgcgaaa atacgcgccc agcgcgccgc tgccgtgatc gtggatgacg atgactcgcg
    12541 gcaatgccgt gagcagcggg ttgtaagggc gcgagtcgca gccgagaaag ccgcagacga
    12601 aatgcgcagc ctcgccgccg tcgctgccca tcgagatctt cgacggcagc ttgccatcgt
    12661 tcggcctgcg gtacatcgac atctcgggtg atttgcgcat gccgggcgtg gtggccagca
    12721 cgtgcgtgtc gccctggggg agcacgatga tgtctcccgc gactagtttg atcggctcct
    12781 ctccttcgag cgtcgcccag caactccctt ccatgagcag gtggtagcag atgaggtgat
    12841 cggagccggg aaacatcgcg tccacgacgg ttttcccgaa cggcgtttcc gcgcaccacg
    12901 gtgcgcacgc gtgcacgtcg aagaagaacg cgccggtcag gcgaaccgca cgcaacacat
    12961 ctgataaagc gtccatgtga cctcccgcat catcgccgga cgttgagaca acaaccccgg
    13021 actggcaatc attcgaaaca gtcttccgtg cggttacttt gtctcacgtg atgattcgta
    13081 atcaacaagt attttctacg gagatgacga tgatgaaagc cttttgcgcc gtgacggcca
    13141 tgacgattgc gggttccagc gccgtcgcga ctgcggcaga gccgtctcag gccacgcacc
    13201 gctacctgat cgaacggacc tttccgcccg gcgccctcga cggtgtcgat gccgccgcga
    13261 agaagaaagt gaacgagaac aatgcgacgc tggatgtcac ctgggagaag tcgtacgcga
    13321 acgccgacaa gaccaagttg tactgcgtgt atgacggccc gaatgaaaac gccgtgcgcg
    13381 aagcggcgaa actcaatggt ctgcccgtcg acaacgtcac cgagatcccc gccgacatca
    13441 aggcggagcc gcgcggcgcc gtgcatcaca tcgcgtccgg caaccagcgc tacctggtga
    13501 agcgctccgg cgcaccggtc gtgacggcca gcagcgacaa gaaattcggc gtgacgctga
    13561 tcacctcgta cgccaccgcg gacaagcgcg atacgttctg ggtatacgaa gcgcctagct
    13621 actcggcggt ggaaagcgcc gccaaggcga gcggcgcgcc gttcgagtcg atcgcggaga
    13681 ttcccgagac tctgtatccg aactgaggtg ttagttaggg ttgtagagtt ggagtgggcg
    13741 gcggcgcaag ccgccgcccg attccaacat ggtccccatt acttcggcgg cgcgagccag
    13801 cggtcgacac cggtggagga gatgaactgc ccctcgatct ccctgaccag gtcggcgtac
    13861 ccctcgcagc cggaattcag caacaccgtg cgcacgtctc cggcgtcggt cgccgagcgc
    13921 atgaccgtga gcacggagcg acccttgagg caggttttgc gtttgatctc ctcgtccaaa
    13981 accttggtcc gcacgagcgt ggtcagcagc tcggcataga cttcgggctc taccttcttg
    14041 ttccagacgc cgaccttgct cgtgcccttg cggccgttga acaccacgcg cccgtcgtcg
    14101 aatacataga gctcgtagac ggggcagttg ccatcgcaag taccgctcga cacggcgacg
    14161 aacttttccc cggggctcca gatcggaatg ccaccgtaag gcgctccggc cgcgtaaggc
    14221 ttgaccaggg gtttttccgc cagcgcgagg ctggtgaatg ccacggtggc aagcgcaaaa
    14281 acgatgagtc ctgttctcat tggatttgtt cctaacattc gacgatgttg acggcgagcc
    14341 cgccgcgcga agtttccttg tacttggtct tcatatccgc acctgtctcg cgcatggtct
    14401 tgatgacctt gtcgagcgag acgtaatgcg tgccgtcgcc tcgcagggcc atgcgtgaaa
    14461 cattgatagc tttggccgcg ccgatggcat ttcgttcaat acagggaatc tgcacgagac
    14521 ctcccaccgg atcgcaggtg agccccaggt gatgctccat ggctatttct gccgcatttt
    14581 cgacctgccg cacggacccg cccagcacgg cggtgagtgc ggcggcggac atggagcacg
    14641 caacgcccac ctcgccctgg cagcccactt ccgcgccgga gatcgaggcg ttctccttat
    14701 ataagaagcc gatcgccgcg gccgtgagca ggaagtccac gatgccggcg tcattcgcgc
    14761 cgtgcacgaa gcgcgcgtag tagtgcatga gcgcgggcac gatgccggcg gcgccgttgg
    14821 tgggtgcggt gaccacgcgg ccgcccgcgg cattctcttc gttcaccgcc agcgcccaga
    14881 ggttcaccca gtcgagcgcg atcaacggat catgcggcgc ggccatctgc ggggccgtga
    14941 gctgcgcatg caatcctggc gcgcggcgtt tcaccttgta accaccgggc agcacgccgg
    15001 tggtttcgca gccgcgcttc acgcaggcct gcatcacatg ccagatgtgc agcagcccct
    15061 ggtcgatttc cgcgtcggta cgccagtggc gttcgttgcg acgcatgatc gccgcgatcg
    15121 tggcgttttc cttctcgcac agcgccagca gttcatcgcc gttgcggaaa ggaaaaggca
    15181 gcacggtggc atcgggcacg atgcgcctgg cggtgccgcc atcaggtgtc tcgctctccg
    15241 aaaccacgaa gccgccgccc accgagtagt aagtgccggt gctcaggccc gtgccatctt
    15301 cggcatatgc cgcgaacagc atgccgttcg aatgcagcgg cagcgtgtcc ttgtggttga
    15361 acgtgagatc gcggcgctca tcgaacgcga ccgcgtggcc accatacagc tgcaactgac
    15421 ccgatgttct gatgctctcg acgatgcccg cgatcgcctc gacatcgacg gtatcgggct
    15481 cgtgaccgga cagccctaac aggatggctt tgtcggtgcc gtgccccttg ccggtggcgc
    15541 cgagcgagcc gtacaacgtg acgctgatgc gcgtcacgcg agtcaactgc tgtgaatcgc
    15601 gcaggcgcgc ggcaaactgc cgggcggcgc gcatgggtcc cacggtgtgc gagctggaag
    15661 gcccgatgcc gatcttgaac aggtcgaaga cgctcaatgc catgcggcat tgtagtccct
    15721 atctggcggg cagctccgca cccagcagct cgatgagccg ctgcggctcg acgggtttgg
    15781 tgaagtgcat gtcgaacccg gcgcccatcg cccgcgcctt gtcctgctcc tggccccagc
    15841 cggtgagggc gatcagcagc gtactgcggc tgcggctgtc gccgcgcact tgccgcgcca
    15901 cctcgtaacc gtccatttcc ggcatcccga tatccagcaa tgccacgtcg ggccgcgcgg
    15961 cctcgatcgc cgccagcgcc tgttgcccgt catgcactac cgtcaccgag tggccttcga
    16021 tctcgagcag catcgcgaga ctctcggcgg cgtcgcgatt gtcatcggcg atcagtaccc
    16081 ggcgtttgcc tggagttgca acggcagcac cgccagccgt gctttgcgtg tggctcgaca
    16141 cttttcggcg cgggatgcga acgaagaact cactgccctt gccgagtccg gcgctgcgcg
    16201 cttcgatcgt gccgccatgc agctcgacga ctcccctgga tagcgcgagc ccgatgccca
    16261 ggccgccttc ggagcgatcc tgcgaggact tcacctgcga gaacatcgag aaaacggcgg
    16321 acagcgcgtc ctgcggcagc ccgacgccgg tatcggtgac ggaaaattcg acgtatttcg
    16381 catccgcggt tgcgcgcagg cgaatggtgc cgcgcgggtc ggtgtacttg gcggcattcg
    16441 tcagcaggtt cgacaacacc tgcgccagcc gcagcggatc ggccgcgaat tgcaccggtg
    16501 ccggcgggag ttcgaccagg aattcatggt gtttcgcttc cagcgccggg cgcgacgtct
    16561 ctaccgcggc atccacgacc gcggtgaggt tggtcatcac cgtccgcagc tcgagcgtgc
    16621 cgcgcgtgat gcgcgaaata tccagcagat cgtcgagcaa cagcgacatg tgatgcactt
    16681 gccgcgtaat gacctcgtgg ctccagcgct tctgcgcctc gctcgcgctc ggggacatgg
    16741 aaatgagcgt ggcctggcgt atcggcgcga gtggattgcg cagttcgtgc gccagcgtcg
    16801 ccaggaattc atccttgcgc tgatccattt cgcgcagcgt ggcttccgcg cgtttctggt
    16861 gcgtgatgtc gcgcgcgatt ttcgacgcgc cgacgacgcg gccgctcctg tccttgatgg
    16921 gcgagaccgt gagcgacaca tcgatgagcg tgccgtcctt gcgcctgcgc gtggtctggt
    16981 aatgatcgat gcgctcgccg cgccgcagac gctcgagaat gccgggctcc tcgttgagtt
    17041 gcttctcggg gatcaggatg gtgacgggtt tgccgatcgc ctcatccgcg gtgtagccga
    17101 agattctctc ggcgccggga ttccacgtgg tgatgatgcc gtcgagcgtc ttggtgacga
    17161 tggcatcgtc ggtatgctcg acgaccgccg ccagccgggc cagcacattc tcggtctgtt
    17221 tgcgttcggt gatgtcctgg atgatgccga tgtactgggc cgcctcgccg gactccttgc
    17281 gcagcagggt gaccgtggtg ctgctccata ccgcgctgcc gtccttgcgc acgtaacgct
    17341 tttccagggc gtagttgtcg atttccccgg cgcgcaattt cttcacgcgc tcgcgcgtcg
    17401 cttccacgtc atcggggtgc gtg
//
LOCUS       DASQJS010000091         4895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1936074, whole genome shotgun sequence.
ACCESSION   DASQJS010000091 DASQJS010000000
VERSION     DASQJS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936074"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1895
                     /locus_tag="VEW08_03665"
     CDS             <1..1895
                     /locus_tag="VEW08_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HYJ39862.1"
                     /translation="AGSWRRCALDYSIDPARHYTPCILDAASLLHRREQFAELVQIAS
                     AEIDWLYDYIAKSGYALVLTDASGIVLYEKTDAALADTFRNAGLMCGADWSERREGTN
                     GIGTCIAENQSVTVHLDEHFRSCHIGLSCSGAPIRDPTGSLVAVLDASTLSAQSTRAG
                     VAHTMALVNLSARLIEKYLFLRHFQDGVVFRFHARPEFVNLQHDGAIALAPDATIVAA
                     DETAMQLLHAENRDALIGRSIDAVFDVNAAELIEPGRRPGGYKDGELQPVRDVALGRR
                     FFASLDRGAMHTHVSIRPAKSARVIQVEAPPARRTSLTLEQLAGEDPQMLRNVRSAYR
                     IAKSRVPVMIEGPTGAGKEVFARALHAASDRAARPFVALNCAAIPESLIESELFGYRP
                     GAFTGARKEGMRGKVLQSSGGTLFLDEIGDMPLALQTRLLRVLEEQEVVPLGTEIPVK
                     VDLHVISASHRNVRSMIERGEFREDLYYRLNGITLELPPLAARSDKERLIREFLAAET
                     SDDRPAAIEVAAFQRLLAYDWPGNVRELRNVIRTALAICDGGVVRLSDLPGELRNEGI
                     RRAAPASQHPAPASTVRDDVLRHIRECDGNMSLTAARLGISRNTLYRRCKRLGIPLRR
                     EHVPLA"
     gene            2065..2262
                     /locus_tag="VEW08_03670"
     CDS             2065..2262
                     /locus_tag="VEW08_03670"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HYJ39863.1"
                     /translation="MVLVCPEGNVQLNSVAAAILGLCDGTRDRTQVVAEVLHRTSNRP
                     RSADIVEFLAAAVARGWILEG"
     gene            2399..4156
                     /locus_tag="VEW08_03675"
     CDS             2399..4156
                     /locus_tag="VEW08_03675"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYJ39864.1"
                     /translation="MVTRVIAVVLTMLASLAANADADQRSRFHLEDATIAQLQAAMSS
                     GRLTSEALTRGYIARILDFDQDGPGVNSVMELNPDALAMARRADDLRRRGRVLGPMHG
                     IPVLLKGNIDTGDRMQTTAGSLGLAGAPAPRDSTVAARLRAGGAVILGKTNLSEWANF
                     RSFNSSSGWSAVGGLTNNPYGINRNACGSSSGSGAAATANFAAVSLGTETDGSIVCPA
                     NANGVVGIKPTVGLTSRAGVVPISHTQDTVGPHGRTVADAAVALAVIQSRAPDGRDPA
                     TADVPLGWKGRARPTNIPADYTQFLNRDGLRGARLGLTRSGIESAPVQVGAAFDEAIA
                     AIEAAGATVVDLDAAGFQFAPADGEFLVLVYEFRDDLRNYLATRTRVPLAGKTLVDAI
                     AFNEANADQEMPFFAQEIFELANSLAPGPDDPQPAFDNMTYNQALEIDRLSGVNGIDK
                     ALADFDLDAVIAPTDNPAWATDLLYGDRFLFGSSGLAAGAGYPIVQVPASMVFGAPLG
                     ISFFGTAFSEPMLIRLASGFEAVTRVRAQNPPSFALMLPDDHIDGTTLRKPRATTSQQ
                     GKQQKAVPQETRSWRPRRM"
     gene            4344..>4895
                     /locus_tag="VEW08_03680"
     CDS             4344..>4895
                     /locus_tag="VEW08_03680"
                     /inference="COORDINATES: protein motif:HMM:NF019145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39865.1"
                     /translation="MSTRPSRTGQGPHSICLYLLPLLLWSGVAPAQGLADDERHYDAR
                     AEQGIARALGQSRAQASTPQSLSADVQELSALELDDITGAVRSLSSERGFLSAATPGE
                     PMTIAMDFVRRNLAALNLEASDLEGYSVSNVVYSKVTGATHIYLQQRHQGIPVYNAQL
                     QINVNRDGRIISVNNSFMAGLHRA"
BASE COUNT          852 a         1610 c         1545 g          888 t
ORIGIN      
        1 ttgcgggttc ctggcggcgc tgcgctctcg actattccat cgatcctgca cgtcactaca
       61 cgccgtgcat cctcgatgcc gcttcgctgt tgcaccgccg cgaacaattc gcggaactcg
      121 tgcagattgc ttcggcggaa atcgactggc tgtacgacta catcgccaag tccggttacg
      181 cattggtcct caccgatgcc agcggcatag tcctgtacga gaagacagac gcggcgctgg
      241 cggacacatt ccggaacgcc gggctgatgt gcggcgcgga ttggagcgag cgtcgcgagg
      301 gaacgaatgg catcggcacc tgcatcgccg aaaaccagtc ggtgactgtc cacctggatg
      361 agcactttcg ctcatgtcac atcggcctct cttgttccgg cgcgcccatc cgggatccga
      421 caggttcatt ggtggccgtc ctcgacgcct ccacgttgag cgcgcaatca acgcgcgccg
      481 gcgtcgcgca cacgatggcg ctggtcaacc tgtccgcgcg cctgatcgaa aagtatctgt
      541 tcctgcggca cttccaggat ggcgtcgtgt tccgttttca cgcgcggccg gagttcgtga
      601 acctccagca tgacggggcc atcgcgcttg cgccggatgc caccatcgtc gcggccgatg
      661 agaccgcgat gcaattgctg cacgcggaga accgcgatgc gctgatcggc cggtcgatcg
      721 acgcagtctt cgatgtcaac gccgcggaat tgatcgagcc cgggcgtcgc cctggtggtt
      781 acaaggatgg cgagctgcaa ccggtgcgcg atgttgcgct gggcagacgc ttctttgcga
      841 gcctcgaccg cggcgcgatg cacacgcacg tgtcgatccg gccggcgaaa tccgcgcgcg
      901 tcattcaggt cgaggctccg cctgcccggc gcacgtcgct gacgctcgaa cagctcgcgg
      961 gcgaggatcc gcagatgctt cgcaatgtgc gcagcgcgta tcggatcgcg aagtcgcgcg
     1021 tacccgtcat gatcgagggg ccgaccggcg ccggcaagga ggtgtttgcg cgcgcgctgc
     1081 atgccgccag tgatcgcgcg gcccgtccct tcgtcgcgct caactgcgcg gcgattccgg
     1141 agtcgctcat cgaaagcgaa ttgttcggtt accgaccggg tgcgttcacc ggcgcacgca
     1201 aggaaggaat gcgaggcaag gtgctgcagt cgtccggcgg cacgttgttt ctcgatgaga
     1261 tcggcgacat gccgctcgcg ctgcagaccc ggctgctacg cgtgctcgaa gaacaggagg
     1321 tcgtgccgct cggcaccgaa attcccgtca aggtggatct gcatgtcatc agtgcatcgc
     1381 atcgcaacgt gcgcagcatg atcgagcgcg gcgaattccg cgaagacctc tactatcgcc
     1441 tcaacggaat cacactcgaa ctgccaccgc tcgccgcgcg ctccgacaag gagcggctga
     1501 tacgcgagtt ccttgccgcc gagaccagcg acgaccggcc cgccgccatc gaagtggcag
     1561 cctttcaacg actgctggca tatgactggc cgggaaacgt gcgagagctg cgcaatgtga
     1621 tccgcactgc gctggcaatc tgcgatggtg gcgtcgtgcg actcagcgac ctgccgggtg
     1681 agcttcgcaa cgagggcatc cgtcgtgccg cccccgcgtc gcagcacccg gctcccgcat
     1741 cgaccgtccg cgatgatgtg ttgcgacaca tccgtgaatg tgacggcaac atgtcgctta
     1801 cggccgcgcg gcttggtatc agccgcaata ctttgtatcg gcgctgcaag cgcctcggca
     1861 ttccattgcg acgggaacac gttccgctgg cttagcgcga cggttcgatg acccgctctg
     1921 gcatgcaacc tgcatattgt tttcctgtcg atcgaatacg gcaggtcatg aaacgtgagg
     1981 agcacgaagg aaggcgctgc gcgcatgtgc atacgcctcg caccggggat ttccctgcgg
     2041 ggccctgagg aaaatggcgc accgatggtt ctggtgtgtc ctgagggaaa cgttcagttg
     2101 aactcggtgg cggccgccat tctcgggctg tgcgacggca cgcgcgatcg cacgcaggtc
     2161 gtggccgagg ttctgcacag aacttccaat cggccgcgct cggcggacat cgtcgaattc
     2221 ctggcggccg ccgtcgcgcg cggctggatc ctggagggct gatcagtcga tccggcctaa
     2281 caactgtcac gtgcgagttg taggcagagt ctgatggact cgtgccggcg cccggcgcag
     2341 attacgccac tcagcgtctt atcgatgtca ttggccgatg tcagcaggga gtgccgccat
     2401 ggtcacacgc gtcatcgcag tcgttctcac catgcttgcg tcgctcgccg cgaatgcgga
     2461 cgcggaccag cgttcacgat tccacctcga ggatgccacc atcgcgcagt tgcaggccgc
     2521 gatgtcctcg gggcgcctga catccgaagc gctcacgcgc ggatacatcg cgcgcatcct
     2581 cgacttcgac caggacgggc cgggcgtgaa ctccgtcatg gagttgaacc cggatgcgct
     2641 cgcgatggcg cgtcgcgcgg atgacctgcg ccgcaggggc agggtgctcg gccccatgca
     2701 cggcatcccc gtcttgctca agggcaacat cgataccggc gatcgcatgc aaactactgc
     2761 cgggtcgctc ggtcttgcgg gcgcgcccgc gccgcgcgat tccaccgtgg ccgccaggct
     2821 gcgcgccggc ggcgcggtca tcctcggcaa gacgaatctc tccgagtggg ccaacttccg
     2881 ctctttcaac tcgtcgagcg gctggtccgc ggtcggcgga ctcactaaca acccctacgg
     2941 catcaaccgc aacgcctgcg gctcgagctc cggctccggc gccgcggcta ccgccaattt
     3001 tgccgcggtg tcgctcggca cggagacgga cggcagcatc gtctgcccgg ccaatgccaa
     3061 tggtgtcgtt ggcatcaagc ccaccgtggg gctcaccagc cgggctggtg tagtgcccat
     3121 ctcgcatacc caggacacgg tggggccgca cggccgcacc gtcgccgacg cggcggtcgc
     3181 gctcgccgtt atccagagcc gcgcaccgga tgggcgcgac cccgcgacgg cagatgtgcc
     3241 gctgggatgg aaagggcgcg cgcggcctac gaacataccg gcggactaca cgcaattcct
     3301 gaatcgtgat ggcctgcgcg gcgcgcgtct cggcctcaca cgttccggaa tcgagtccgc
     3361 accggtgcag gtgggggcgg cattcgacga agcaatcgcg gcaattgaag ctgccggcgc
     3421 aacggtagtc gatctggatg ccgccggatt tcaattcgcc ccggccgatg gcgaattcct
     3481 cgtgctggtc tacgagtttc gcgacgacct gcgcaattac ctcgctacgc gcacgcgcgt
     3541 accgctcgcg ggcaagacgc tggtcgacgc catcgcgttc aatgaagcga atgccgacca
     3601 ggaaatgcca ttcttcgcgc aggagatttt cgagctcgcg aactcactcg cacccgggcc
     3661 ggacgacccg caacctgcct tcgacaacat gacgtacaac caggcgctgg agatcgaccg
     3721 gctgtcgggc gtgaatggca tcgacaaggc gctcgcggat ttcgatctcg atgcggtcat
     3781 cgcgccgacc gacaatccgg cgtgggcgac ggacctgctc tatggcgacc gtttcctgtt
     3841 cggcagctcc ggtctcgcgg ccggcgcggg ttaccccatc gtgcaggtgc cggcgagcat
     3901 ggtgttcggc gcgccgctcg gcatcagctt cttcggcacg gcattcagcg agcccatgct
     3961 gatccgcctc gcatcaggct tcgaggccgt cactcgcgtg cgcgcacaga atccgccgtc
     4021 cttcgcgttg atgctgccgg atgatcacat cgatggcacg acgctccgga agccgcgcgc
     4081 cacgaccagt caacagggga agcagcagaa ggcggtgccg caagaaacga ggtcgtggcg
     4141 cccgcggcgc atgtagacgg ctcttcggca attcctcgcg gctgctgcat cgccaccgcg
     4201 gcagttacta cgtattgacc ctgcatcggg cctgtctgag gatcgccgcg ccttacgtcg
     4261 cccaagggag gttcattgac gtatggcccg ctcgcgcaac cagcgcagag cttcccttcg
     4321 ctcaactgaa aaggatattc atcatgagca cacgtccatc gcggaccggc cagggtccgc
     4381 attccatctg tctctatctg cttcctcttc tgctttggtc cggcgtggca ccagcacaag
     4441 gtctcgccga tgacgaacgt cactacgatg cgcgtgcaga gcagggcatc gcgcgagcac
     4501 tcggacaatc ccgtgcgcaa gcatcgacgc cgcagagcct ctctgccgac gtgcaggaac
     4561 tttcggcgct ggagctcgat gacataaccg gcgcggtgcg ctcgctttcg agcgaacgcg
     4621 gctttctcag tgccgcgacg ccgggcgaac cgatgaccat cgcgatggac ttcgtccgca
     4681 ggaatctggc ggcactgaat ctcgaggcgt ccgacctcga gggttactcg gtatcgaacg
     4741 tcgtgtattc gaaggtcacc ggggccactc atatatatct gcagcagcgg caccagggca
     4801 ttccggtcta caacgcgcag ttgcagatca atgtgaatcg tgacggccgc atcatcagcg
     4861 tcaacaactc tttcatggcc ggattgcacc gcgcg
//
LOCUS       DASQJS010000092         3316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_363560, whole genome shotgun sequence.
ACCESSION   DASQJS010000092 DASQJS010000000
VERSION     DASQJS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363560"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VEW08_03685"
     CDS             complement(<1..621)
                     /locus_tag="VEW08_03685"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HYJ39866.1"
                     /translation="MNAALLILAGGILAAVIGIVIWLRATRQKPKPVDPIESTTGSNR
                     TLTGTQPRGSVASLSWDEALRRLVAFALDDVPREALSRTPDPEHAPVFKAVQQILERI
                     EARPEYIPRRPSLLPKLMATVNDNEATMAEMSRIIAQDPALTGNLLRIANSPIYRTGS
                     LPVDSIDRAVTLVGVQGIRSIIATALLQPVMTSGSSAFSKFPELVWE"
     gene            967..2361
                     /locus_tag="VEW08_03690"
     CDS             967..2361
                     /locus_tag="VEW08_03690"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYJ39867.1"
                     /translation="MEARLLYCDSESSRIDPESRAKLARAGIEAAAANCAAACPTLLF
                     FCESGPALLQKIAALRAAGTERVLALGVGAPPDQSFAWQLLCAGASDLLTWNDTDEVA
                     MAVAARLTRWREIDELVDSPLIAGSLVGKSPRWQSTLRQVVEVARFTAASALILGESG
                     TGKELIARLFHSLDPRQDKGNLVIVDCTTIVPELSGSEFFGHERGAFTGACGTRDGAF
                     ALARGGTVFLDEVGELPLALQAQLLRAVQEQTFKRVGGNTWFKTDFRLVCATNRDLQA
                     AVHRGEFRHDLYHRIANWVFTLPPLRERTEDIVPLARHFVQQRRAGRKPLELDATVEQ
                     CLLRRHYPGNIRELRQLVMRIMDRHVGDSSITVGDLPLVERPDDAPGNEWRDDSFLRS
                     IGLALSSGVGMKEIGRAAEDSAIQLALREAGGNLQGAASRLGVTDRALQLRRATARQA
                     QRPDTQPQQVPEIS"
     gene            complement(2331..3206)
                     /locus_tag="VEW08_03695"
     CDS             complement(2331..3206)
                     /locus_tag="VEW08_03695"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ39868.1"
                     /translation="MNVNSSSIIDCHCHAGTGDGLTGPWDTRASLTAYLRRASAAGIT
                     HTNLFAAFHTDYAQANQEVARIVASRPRQFYGFAFVNAVRDLGRVRQLVQTAVQRYGF
                     VGIKVHRHEGRITREICEVARAFGLPVLYDVMGEVSACELLAREYPDVPFIIPHLASF
                     AGDWRAHLALIDQLVRHRNMHADTSGVQRFDLLAEAVQRAGARKILFGTDGPWLHPGV
                     ELAKVRALQLSVGDERRILAQNFLRLIDGVVAQPRKTPRPISAVTATIAWQPEASDPW
                     QRPAAAQEISGTCCG"
BASE COUNT          563 a         1064 c         1104 g          585 t
ORIGIN      
        1 ctcccagacc agctcgggga atttgctgaa ggcagatgag cccgaggtca tgaccggttg
       61 cagcaacgcg gtggcgatga tcgagcgaat cccctgcacg ccgaccagcg tcaccgcgcg
      121 gtcgatgctg tccacgggaa ggctgcccgt tcgatagatc gggctgttcg cgatgcgcag
      181 caggttgccg gtgagcgccg gatcctgcgc gatgatgcgc gacatctcgg ccatggttgc
      241 ttcgttgtcg ttgaccgtcg ccatcagttt cggcagcagt gatgggcgac gcggaatgta
      301 ttcgggacgc gcttcgatgc gttcgaggat ttgctgcacg gctttgaata cgggcgcgtg
      361 ttcgggatcg ggcgtccggc tcagcgcctc gcgcggtacg tcgtcgagcg cgaaggccac
      421 gagccggcgc agcgcctcgt cccagctcag cgatgccacg gatccccgag gctgcgtgcc
      481 ggtcagtgtg cgattcgagc ccgtggtcga ttcgatggga tcgaccggtt tgggtttttg
      541 ccgggtagcg cgaagccaga tgacgatgcc tatcaccgcc gcgaggattc cgccagccag
      601 aatcagaagg gctgcattca tggaggcgag gctacccggt caggggggca ggccgctacc
      661 cgcttgtgcg ccgccacttt ttgacttttc ccaacgtggc caagcacttc aaaccgaccg
      721 cttgtctcct gacgtcgtcg ccgcgagccc tcactcgatc agcgcgtgca acgaactcat
      781 cgccgtcgac catcgcgcac cgccatggcg cggcgacggt ctaacttctc gcgcgagtgc
      841 ccgagtgtcg cgttgccaca ggaaatatgt ctcggctttg gaaaaaactc cgcagatggt
      901 acggcgggaa ctgcatccac ccggcttatt ctcgaagcga catcgaagtg acatggacgc
      961 tgcaaggtgg aagcacggct gttgtattgc gatagcgaga gttcccgcat cgaccccgag
     1021 tcgagagcga agctcgcgcg cgccggcatc gaagcggccg ccgccaattg cgccgctgcc
     1081 tgcccgacgc ttctcttctt ttgcgagtct gggcctgcat tgctgcagaa gattgctgcg
     1141 ttacgcgctg caggcaccga gcgcgtcctt gcactcggcg tgggcgcgcc acctgaccag
     1201 tcattcgcgt ggcagctgct gtgtgccggc gcatccgatc ttctgacgtg gaacgacacg
     1261 gacgaagtgg cgatggcggt cgcggcgcgc ctcacgcgct ggcgcgagat cgacgagttg
     1321 gtggactcgc cgctgatcgc cggatcgctg gtgggcaaga gtccgcgctg gcaatcgacg
     1381 ctgcgccagg tggtcgaggt tgcgcgcttc accgcggcca gcgcgctgat actcggcgaa
     1441 agcggcaccg gcaaggaact catcgcgcgg ctcttccact cgctcgaccc gcgccaggac
     1501 aagggcaatc tcgtcatcgt cgattgcacg accatcgtgc ccgagttgtc gggcagcgaa
     1561 ttcttcggcc acgaacgcgg ggcgtttacc ggcgcctgtg gcacgcgtga cggcgcattc
     1621 gcactggcgc gcggcggcac cgtgttcctc gacgaagtgg gcgaattgcc actggcgctg
     1681 caggcgcagc tgttgcgcgc cgtgcaggaa cagactttca agcgcgttgg cggcaatacc
     1741 tggttcaaaa ccgatttccg gctggtgtgc gccaccaatc gcgatctgca ggcggcggtg
     1801 catcgcgggg agtttcgcca tgacctctac catcgcatcg ccaactgggt attcacgctg
     1861 ccgcccttgc gcgaacgcac ggaagacatc gtgccgctgg cgcggcactt cgtgcagcag
     1921 cgccgtgccg gccggaaacc gctcgagctc gatgccacgg tcgagcagtg cctgctgcgg
     1981 cgtcactacc cgggcaacat ccgcgagctg cgccagctgg tgatgcgcat catggatcgg
     2041 catgtgggcg acagctcgat cacggtcggc gacctgccgc ttgtcgaacg ccccgacgac
     2101 gcgcccggaa acgagtggcg tgatgactcg ttcctgcgct ccatcgggct cgcgttgtca
     2161 tccggcgtcg gcatgaagga aatcggccgc gccgcggagg acagcgccat ccagctggcg
     2221 ttgcgcgaag ccggaggaaa tctgcaaggc gccgcgagcc gtctcggggt aaccgaccgc
     2281 gcattgcagc ttcgccgcgc cactgcgcgc caggcccaac gacccgacac tcaaccgcag
     2341 caggtgcccg aaatttcctg agcagccgcc ggacgctgcc acggatcgct cgcttccggt
     2401 tgccaggcaa tcgtcgcggt aaccgccgag atcggacgcg gcgtttttcg cggctgcgcg
     2461 acgacgccat cgatgaggcg caggaaattc tgcgcgagga tcctgcgctc gtcgcccacg
     2521 gacagctgca gcgcccgcac cttggcgagc tcgacaccgg gatgcagcca gggaccgtcc
     2581 gtgccaaaca ggatcttgcg cgcgccggcg cgctgaaccg cttccgccag caggtcgaat
     2641 cgctgcacgc cggaagtatc ggcatgcatg ttgcgatggc gaaccagctg gtcgatgagc
     2701 gccaggtgcg cgcgccagtc gccggcaaaa cttgccaggt gcgggatgat gaagggcacg
     2761 tcggggtatt cgcgcgccag caattcgcac gccgacactt cgcccatgac gtcgtagagc
     2821 accggcaggc cgaaggcgcg cgccacctcg cagatctcgc gcgtgatgcg cccctcgtgc
     2881 cggtgcactt tgatgccgac gaagccgtag cgctgcacgg cggtctgcac cagttgccgc
     2941 acccggccca ggtcacgcac cgcgtttacg aaagcgaagc cgtagaactg ccgtggccgg
     3001 ctcgcgacga tgcgtgccac ttcctgattg gcctgcgcat agtcggtgtg aaatgccgcg
     3061 aacaggttcg tgtgcgtgat gcccgcggca gaggcacggc gtagataggc cgtgagcgag
     3121 gcgcgtgtgt cccagggacc cgtgaggcca tcgccggtgc ccgcgtggca gtggcagtcg
     3181 atgatcgatg acgagttgac gttcatctca ggggttgttg ttcttgatga gcgtgtcgat
     3241 atcacgcacc agcgcgtaca tggtgtcgtc ggaaaccggc tgacctttgg cctcggtgag
     3301 aatggccacc gcgtaa
//
LOCUS       DASQJS010000093         9414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3195754, whole genome shotgun sequence.
ACCESSION   DASQJS010000093 DASQJS010000000
VERSION     DASQJS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9414
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3195754"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VEW08_03700"
     CDS             <1..561
                     /locus_tag="VEW08_03700"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HYJ39869.1"
                     /translation="WHVRGIQLSANLQLRQGLDAFGSGLEAADLVHDPRRADFLVTLM
                     QASAYRRFADSWSLRFDGFLQNSGYVLPDSERFKIGGDRLGRGFEVAEIAGDSGIGGK
                     LELRRDLINTESVIGRLSTYGFYDIGAAWKQDAPGRESAATAGAGFAMQGAALTGYLE
                     VASPLTGPDIEGKQTASVFAEISYRF"
     gene            616..1152
                     /locus_tag="VEW08_03705"
     CDS             616..1152
                     /locus_tag="VEW08_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39870.1"
                     /translation="MLLERLDPLDRVLDMHAAALGADFTAYRNHTYRVVNFYAALSPC
                     AGAALEKVALAAAFHDLGIWTAGTFDYLQPSITLACDHLECAGHGDWRDTVAGMIREH
                     HKIRRFQGAAAEFIEPFRRADWIDVSLGVLSFGLPRERLRKILARWPNAGFHKRLAQL
                     TVRRLRTHPLSPIPVLKW"
     gene            1146..1871
                     /locus_tag="VEW08_03710"
     CDS             1146..1871
                     /locus_tag="VEW08_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39871.1"
                     /translation="MVGARSLLLALAAFVASAHAGESLVTTERSATLASLFAPDDVVG
                     LSRTLPPDKPVKFRVWPSESKNPGVLIFVSPTNSGEPPAGWLPLLQQKDLSWIAADGF
                     GNQELSAQRILVAVMALKLAQQSMQTDAKRTYIGGMSGGGRIASLTATRFPQMFSGAL
                     YIVGANFWMPKDARLKPLLAANRYVFITGERDFNRSEMRRVFSRYQSNGLTASLLMDL
                     PAFAHEYPNAEQLGQAINFLDSR"
     gene            complement(1868..3229)
                     /locus_tag="VEW08_03715"
     CDS             complement(1868..3229)
                     /locus_tag="VEW08_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYJ39872.1"
                     /translation="MNATDASVSATAKLSKSLRARHLIMISIGGIIGAGVFVGSSAAI
                     QTIGPAVILSYISAGIVVYAVIRILARMALDQPGLGSFTEYVRAGLGDGAGFIAGWLY
                     WWFWVVVVAIEALAGAKTIHVWLPNFEPWQIGLALMAVLTAVNLASARSYGEFEFWFA
                     SIKVAAIIVFIVVAASWLLGVGNPASPGLSNLTTQGGFAPYGWGAIFAGITTVIFSMV
                     GAEIVTVAAAESAESNRTMSRLAATVVLRILLFYVLSILLILCIVPWTEFKSGDSSFA
                     LALERIGIPSAATIMNVIVLTAVLSCLNSGVYVTARVLFAMADKGDAPRWLTAVNSRQ
                     VPARAILLGSAFGFLVMALNWIAPEKLFTFLLNSCGALMMFTYLFVAVAHFRFPYSRP
                     ELRDAGGAKFIAGGAALAMIAVMVAMTVMPSKQPEMWASLACLGVIVVALFVKRAVTD
                     RPS"
     gene            3381..4295
                     /locus_tag="VEW08_03720"
     CDS             3381..4295
                     /locus_tag="VEW08_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ39873.1"
                     /translation="MKLTGNTRWAGALAALTLMALPAAAAEKKTGYAKANGLNYYYEV
                     SGKGEPLLLLHGGLGSTDMFQPILPALTDHRTVIAVDLQGHGRTQLGTRKFSLPDLGD
                     DLAVVLKQLGYSQVDVFGYSFGGGVGFRLAAQHPAMVRRLAIVSTGFSDDGFYPEIRE
                     QQKQMNAGFAEMMKETPMYKSYVAVAPRPEDFPRLLQTIGDLMREHYDYSADVARLTM
                     PTMLIFGDADMYRPEHIVEFYKLLGGGLRDAGWQRETISKNRLAILPDQTHYDIFFSP
                     KLVDAALPFLDGVSGSKSWEQTLDKTKN"
     gene            complement(4404..7397)
                     /locus_tag="VEW08_03725"
     CDS             complement(4404..7397)
                     /locus_tag="VEW08_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ39874.1"
                     /translation="MSRSDRSSFLLRHAVSLAISGASLALPMQAIADATDAAEESQEL
                     EEVVVTGFRGSLNTAITAKRSENAAIDVIASEDIGKFPDSNLAESMQRVPGVALARGD
                     GGEGRNISVRGLAPAFTKVRINGMEGAAQTGSSDIYGAGNNGRAFDFNVFPTEIFSQL
                     AVRKTASADVEEGSLGATVDLKAPSAFDYGDNQVFSITGRGIYNEVSEDVDPRVSMLA
                     SKKFFDDTFGVMATFSFQERHTREAGYSAVDILHFGTNANNIGTSTAPILLPFCTPIG
                     WTLTAPSPVPGTRGATGTDCSAGNARTSDTAAFMEVYNRTNDAIRDAAGNLVPGGGAY
                     HPRLPRFVNSEQDTERSGGSLSLQWRPTENTSISLDGLLSRYQQERRDDYILALSFGR
                     NLSNNGQPMVSIKDVEFDDNGSMTYGLFDGVDVRAEGLVDQFVSTYEQLSLDMNHQFN
                     DSFSVHAYAGRSVNDWDGPLRFQTFIDAIDTENFSVDFRGGRDVPIIGFGFDVSNPAN
                     FQYAPTPDNNQTVLGGFSLQGKPQENITANNTFELDGSWQMSDAWKVKVGVQYRESHF
                     SSHGSNPLRNATLTRSLPAGVSVGDITRQISGLDDIFGSGAPPSWAAVDLDKWHEVFD
                     IDSIGVCAAPTCGAAQSKIFEDVQTGYLMFNFDSGDRWSMPIRGDFGVRYVMTDQFAS
                     GIIPVSAPGTAPVTSFAQYNEVERDYTDTLPSFNIVFELTDNLLARLSGSKVMSRPEL
                     GNLAPTSGVTATTRTGNVNNPYLDPIRADTADLSLEWYFSEGSLLSVAYFYKDIESYI
                     QRVTEQVPYRELGLPDSLLDGTPASPTDIFTVGRFQNTPGGPLEGFEINAQVQFSFLP
                     GFWSNFGVLANYTEVDAEIEYFISTTSPPVTAQLVGMSPTTAAGTLYYENDKFSIRTT
                     ASYRDKFFRAIPASPGSDVRGDLSTTYVDAQASWFVNDNLSILLEAQNLTGERSTLYI
                     DDTRENTLFQTEVGTTYTLGATFKF"
     gene            complement(7728..7979)
                     /locus_tag="VEW08_03730"
     CDS             complement(7728..7979)
                     /locus_tag="VEW08_03730"
                     /inference="COORDINATES: protein motif:HMM:NF037968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HYJ39875.1"
                     /translation="MNPEWLGFAAAALTSASFIPQAVMTIRSRDTRGISRGMYIIFVI
                     GVALWLAYGIYLDSLPMIIANVVTLGLAGTILALKLRYG"
     gene            complement(8100..8175)
                     /locus_tag="VEW08_03735"
     tRNA            complement(8100..8175)
                     /locus_tag="VEW08_03735"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8140..8142),aa:Phe,seq:gaa)
     gene            8308..9171
                     /locus_tag="VEW08_03740"
     CDS             8308..9171
                     /locus_tag="VEW08_03740"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HYJ39876.1"
                     /translation="MNTAAKLATTPRAVIQTLRGQPASDGAGVKLTRVIGQPRLPDLD
                     PFLLLDEFGTDKAEDYIAGFPEHPHRGFETVTYMLDGRMRHKDNHGNEGVLEPGAVQW
                     MTAGRGIVHSEMPEQREGRMRGFQLWMNLPAKDKMTAPNYQEFGGDKLPVVERTGVSV
                     KVIAGSLDGATGPVRQPATDPVYMDIRLDAGADFTLPLPVGHSAFVYVYEGGVSVGVS
                     REAATIHAQELAVLGDGSEVRLQGREARSRAILVAGRPLREPVARYGPFVMNTREELH
                     QAFADFQAGKF"
BASE COUNT         1818 a         2885 c         2986 g         1725 t
ORIGIN      
        1 tggcacgtga gaggaatcca gttgtcggcg aacctgcaat tgcgccaggg gctggacgcg
       61 ttcggcagcg gtctcgaagc agccgatctg gtccacgacc ctcgccgcgc cgattttctc
      121 gtgacgttga tgcaggcgag cgcgtaccgg cgcttcgccg atagctggtc gttgcggttc
      181 gatggcttcc tgcagaacag cggctacgtg ctgcccgaca gcgagcgttt caagatcggc
      241 ggcgaccggc tcgggcgcgg attcgaagtc gccgagatcg ctggcgacag cggtatcggc
      301 ggcaagcttg agctgcgccg tgatctcatc aataccgagt cggtcatcgg ccgcctttct
      361 acgtatggct tctatgacat cggcgccgcc tggaagcagg acgcgccggg tcgcgagtcc
      421 gcggccaccg cgggcgcagg tttcgcgatg cagggcgccg cgctcacggg ctatctcgaa
      481 gtcgcctcgc ctctcaccgg ccccgacatc gaaggaaaac agacggcgtc ggtgttcgcg
      541 gagatcagct accgcttcta aaatgttgct ataacttcgt cctcccaaac aacaatcaga
      601 acggggacga aggatgtgct gctagaacgc ttagacccgc tcgaccgcgt gctcgacatg
      661 catgcggccg ccctgggcgc cgacttcacc gcgtatcgga atcacaccta tcgcgtggtg
      721 aatttctacg cagcgctctc gccctgcgcc ggtgccgcgc tcgagaaggt cgcgctcgcc
      781 gcggcgttcc acgatctcgg catctggacc gcgggcacct tcgactatct tcagccgtcc
      841 atcacgcttg catgcgacca cctcgaatgt gcgggtcacg gcgattggcg cgacactgtc
      901 gccggcatga ttcgcgagca tcacaagatc cgcaggtttc agggcgccgc ggccgagttc
      961 atcgagccat tccgccgcgc ggactggatc gacgtctcgc tcggagtatt gtcattcggg
     1021 ctgccgcgag aacggctgcg caaaatcctc gcgcgctggc ccaacgcagg atttcacaag
     1081 cggctcgcgc agctcacggt caggcggctg cgcacacatc cgctgagtcc catcccggtg
     1141 ctgaagtggt aggcgcgcgg agcctgttgc tcgcgctcgc agccttcgtg gcatcagcgc
     1201 atgcggggga atcactggtt accaccgaac gcagcgccac gctcgcgtcg ctgttcgcgc
     1261 cggacgacgt cgtgggtctg tccaggaccc tgccgccaga caagccagtc aagttccgcg
     1321 tgtggccgtc ggagtcgaag aatcccggcg tgctgatatt cgtgagtccc accaactccg
     1381 gcgagccgcc cgccggatgg ctgccgcttt tgcagcagaa ggatctgagc tggatcgccg
     1441 cagatggatt cggcaaccag gaactgagcg cgcaacggat tctcgtggca gtgatggcac
     1501 tcaagctggc tcaacaatcg atgcagacgg acgcgaagcg aacctacatc ggtggcatgt
     1561 cgggcggtgg tcgcatcgca agcctgacgg ctacccgctt tccacagatg ttctccgggg
     1621 cgctgtacat cgtcggcgcg aatttctgga tgccgaagga cgctcggctc aagccgctcc
     1681 tggctgcgaa ccgctacgtg ttcatcaccg gtgagcggga tttcaatcgc agcgagatgc
     1741 ggcgcgtctt ctcccgctac cagtcgaacg ggctgactgc ttcattactc atggatctac
     1801 ctgcgttcgc gcatgagtac ccgaatgccg aacagctcgg gcaggcgatc aactttctcg
     1861 attctcgtta ggacggtcgg tcggtcacgg cccgcttcac gaacagcgcc acgacgatca
     1921 cgccgaggca ggcaaggctt gcccacatct ccggttgttt gctgggcatt accgtcatgg
     1981 cgaccatgac ggcgatcatg gccagcgccg ctccaccggc gatgaacttc gcgccgccgg
     2041 catcgcgcaa ctcggggcgc gaatacggaa aacggaagtg cgccaccgcc acgaacagat
     2101 aggtgaacat catcagggcg ccgcaggagt tcagcaggaa tgtgaacagc ttttccggcg
     2161 cgatccagtt gagcgccatc accaggaatc caaatgcact gcccagcagg atggcgcgtg
     2221 ccgggacctg acgagaattc accgccgtga gccagcgtgg cgcatccccc ttgtcggcca
     2281 tggcgaacag cacccgtgcc gtgacgtaga caccggagtt gaggcatgac agcacggccg
     2341 tcaacacgat gacgttcatg atggtcgccg cgctcgggat gccgatgcgc tcgagcgcca
     2401 gtgcgaatga ggaatcgccg gacttgaact cggtccacgg cacgatgcac aggatcagca
     2461 ggatcgacag cacgtagaac agcaggatgc gcaggaccac ggtggccgcg agacgcgaca
     2521 tggtccggtt cgattccgct gattccgccg ccgccacggt gacgatctcc gcgcccacca
     2581 tggagaagat cacagtggtg atgccggcga agatagcgcc ccaaccatag ggcgcgaaac
     2641 cgccttgcgt ggtcaggttc gaaagaccag ggctggccgg attgccgaca cccaacaacc
     2701 acgacgccgc gacgacgatg aacacgatga ttgcggcaac cttgatagac gcgaaccaga
     2761 attcgaattc gccataggaa cgcgccgagg cgagattcac ggcagtcaga accgccatca
     2821 gcgcaaggcc gatctgccac ggttcgaagt tcggcagcca gacgtgaatg gtcttggccc
     2881 cggcgagcgc ctcgatggcc acgaccacca cccagaacca ccagtacagc caaccggcga
     2941 tgaatcccgc gccgtccccc agacccgcgc gcacgtattc ggtgaacgag ccgagacccg
     3001 gctgatcgag cgccatgcgc gcgaggatgc ggatcaccgc gtagacgacg atacccgccg
     3061 atatgtagct gaggatcacg gctggcccga tggtctggat cgcggcgctg ctgccgacga
     3121 atacgccggc gccgatgatc ccgccgatgg agatcatgat gagatggcgc gcgcgcaggc
     3181 ttttcgagag tttcgcggta gcggagacgg aagcgtcggt ggcgttcaag attgttcgct
     3241 ctttttgtgt cgtgacgacg gagcgcacca tacgccgagg aggctgagaa ttaaaaggga
     3301 gagacatctc cagtcgaaaa cacagcctcc ggttcgacca gtcagtgttc aaccagaaaa
     3361 cactgaaaaa ggaagcgatc atgaaactga ctggaaacac gcgctgggcc ggggccctgg
     3421 ctgcgctgac gctgatggcc ttgccggccg ccgccgccga gaagaagacc ggctacgcga
     3481 aagccaacgg cctcaactat tactacgagg tctcaggcaa gggggagccc ctgctcctgc
     3541 tgcacggcgg cctcggttcc accgacatgt tccagcccat cctgccggct ctcaccgacc
     3601 accgcacagt cattgccgta gacctgcagg gtcatggccg cacgcagcta ggcacgcgca
     3661 aattcagtct tcccgatctc ggcgatgacc tggccgtggt gctgaagcag ctcgggtatt
     3721 cgcaggtgga cgtattcggc tattcgtttg gcggcggcgt cggttttcgc ctggcggccc
     3781 aacatccggc gatggttcgc cggctggcga tcgtgtccac gggtttttct gacgatggct
     3841 tctatccgga aatccgcgaa cagcagaagc agatgaacgc gggtttcgcc gagatgatga
     3901 aagagacccc gatgtacaag tcatatgtgg cggtggcgcc caggcccgag gattttccgc
     3961 ggctcttgca gaccatcggc gacctgatgc gtgagcatta cgactactcg gcggacgtcg
     4021 ccaggctcac catgcccacc atgctgattt tcggtgacgc cgacatgtat cggcccgagc
     4081 acatcgtaga gttctacaag ctgctgggcg gcgggctgcg cgacgcgggt tggcagcgcg
     4141 aaacgatttc gaagaaccgt ctcgccatcc ttcccgacca gacgcactac gacatcttct
     4201 tctcgccgaa gctggtcgat gcggcgctgc cctttctcga tggagtcagc gggtcgaaga
     4261 gctgggaaca gaccctggac aagacgaaga actgaaaaca ctacagccaa aaaaaagggg
     4321 ccttgcggcc ccttttctgt atttcacttc aggcacgccg ccccttcagg gcggcgtttt
     4381 ccgaagtggg cttcagccct gaactagaac ttgaaggtag cccccaacgt gtacgtagta
     4441 ccgacctcgg tctggaacaa cgtgttctca cgcgtgtcgt cgatgtacag cgtgctgcgc
     4501 tctcccgtca ggttctgcgc ctcgagcagg atcgagaggt tgtcgttcac gaaccaggag
     4561 gcctgcgcat ccacgtaggt cgtcgagagg tcgccacgca cgtcactgcc aggcgaagcc
     4621 ggaatcgctc ggaagaactt gtcacgatac gaagccgtgg tgcggatgct gaacttgtcg
     4681 ttctcgtagt acaacgtgcc ggccgcagtg gtgggcgaca tgccgaccag ttgcgcggtc
     4741 accggagggc tggtggtgct gatgaagtac tcgatctcgg catcgacttc cgtgtagttg
     4801 gccagcacgc cgaagttgct ccagaaaccc ggcaggaagc tgaactgcac ctgcgcgttg
     4861 atttcgaagc cttccagcgg accgcccggc gtgttctgga agcgtcccac cgtaaagatg
     4921 tcggtgggcg aggccggcgt gccgtccagc agcgagtccg gcagtccgag ctcacggtac
     4981 gggacctgct cggtgacgcg ctggatgtaa ctctcgatgt ccttgtagaa gtaggccacc
     5041 gacagcaacg agccttccga gaaataccat tccagcgaca gatccgctgt atcggcgcgg
     5101 atgggatcga ggtatgggtt gttcacgttg ccggtacgcg tggtcgccgt gacgcccgag
     5161 gtcggcgcca ggttgcccag ctccggacgc gacatcacct tcgagcctga gaggcgcgcg
     5221 agcaggttgt cggtaagttc gaaaacgatg ttgaacgacg gaagagtgtc cgtgtagtcc
     5281 ctctccacct cgttgtactg cgcgaaggac gtaaccggcg ccgttccggg ggcggagacc
     5341 gggatgatgc ccgaagcaaa ctggtcggtc atcacgtaac gcacgccgaa gtcgccgcgg
     5401 atcggcatgc tccagcgatc acccgagtcg aagttgaaca tgaggtagcc cgtctgcacg
     5461 tcctcgaaga tcttgctttg agcggcgccg catgtcggcg cggcgcagac tccgatgctg
     5521 tcgatatcga agacctcgtg ccacttgtcg aggtcgacgg ccgcccagct cgggggtgcg
     5581 cctgatccga aaatgtcgtc gagaccgcta atctgcctgg tgatatcgcc taccgaaacg
     5641 cccgcgggca gactccgcgt gagcgtggcg ttccgcagcg ggttcgaacc atggctgctg
     5701 aagtgactct cgcggtattg aacgccgacc ttcaccttcc acgcgtcgct catctgccag
     5761 ctgccgtcca gctcgaaggt gttgttggcc gtgatgtttt cctgcggctt gccttgcagc
     5821 gagaagccac cgagcacggt ctggttgttg tccggcgtcg gtgcgtactg gaaattggcc
     5881 gggttcgaaa cgtcgaagcc gaagccgatg attggcacat cgcggccacc gcggaagtcc
     5941 accgagaagt tttcggtgtc gatggcatcg atgaaggtct ggaaacgcag cggcccgtcc
     6001 cagtcgttga ccgagcggcc ggcgtaggca tgcacggaga agctgtcgtt gaactggtga
     6061 ttcatgtcca ggctcaactg ctcgtacgtc gagacgaact ggtcgaccag gccttcggcg
     6121 cgcacgtcga cgccgtcgaa caggccgtag gtcatcgatc cgttgtcatc gaactcgacg
     6181 tccttgatcg acaccatcgg ctggccgttg ttgctgagat tgcggccgaa agagagcgcg
     6241 aggatgtagt cgtcgcggcg ttcctgctgg tagcgcgaga gcaaaccgtc cagcgagatg
     6301 ctggtgtttt cagtgggacg ccactgcagc gagaggctgc cgcccgaacg ctcggtgtcc
     6361 tgttccgagt tcacgaagcg cggcaggcgc ggatggtagg cgccgccgcc gggcacgagg
     6421 ttgcccgcgg catcgcggat cgcatcgttg gtgcggttgt agacctccat gaacgcagcc
     6481 gtgtcgctgg ttcgtgcatt gccagcgctg caatcggtgc ccgttgcgcc gcgcgtaccc
     6541 ggaaccgggc tcggcgcggt gagcgtccag ccgataggcg tgcagaacgg caggagaatt
     6601 ggcgcggtgc tggtgccgat gttgttggcg ttggttccga aatgcagaat gtccaccgcc
     6661 gagtagcccg cttcacgcgt gtggcgttcc tggaacgaga acgtggccat caccccgaag
     6721 gtgtcgtcga agaacttctt cgacgccagc atcgacacgc gcggatccac gtcttcactg
     6781 acttcgttgt agatgccgcg gccggtgatc gagaacactt ggttgtcgcc gtagtcgaag
     6841 gcactcggcg ccttgaggtc gacggtcgcg cccagcgaac cctcctcgac atccgccgaa
     6901 gccgtcttgc gcacggcgag ctgcgagaag atttccgtcg ggaaaacatt gaagtcgaag
     6961 gcgcggccgt tgttgccggc gccgtagatg tcactggagc cggtctgcgc ggcgccttcc
     7021 atgccgttga tgcgcacctt ggtgaaagca ggggcgagac cgcggaccga gatgttgcgg
     7081 ccttcgccgc cgtcaccgcg cgcgagtgcg acaccgggga cgcgctgcat ggattcggcg
     7141 aggttgctgt ccgggaactt gccgatgtcc tccgacgcga tgacgtcgat ggccgcgttc
     7201 tcgctacgtt tggctgtgat cgctgtattc agcgaaccgc gaaagccggt cacgacgacc
     7261 tcttcgagct cctgagattc ttcagcggcg tccgtcgcat ctgcgatcgc ctgcatcggg
     7321 agcgccagac tggcgccgga aatcgccagc gagaccgcat ggcgtaatag aaaagaagaa
     7381 cggtcagacc tggacattga attacccccc aattaaccgt ggtttcgaga tcaatctgaa
     7441 aaaccgcgca gtcatccgaa cgcaggctgg gtgatgaagg ccccgaacct gatcggtttt
     7501 gaccgattta taccgtatat gagcctgatt gatcccggtt attgaccgcg tgtcaagtga
     7561 attgtccacc ggtggcattg attgttgcga aacacccaca aatggatcaa ctctttgaat
     7621 agagaagtgt ggattcagcg cttctggcgc ttttaccgcc tttcgcgggc gggtgcgaga
     7681 tccgagatga atgatggttc gataccagtt tcggtatcag accagcgtca accgtagcga
     7741 agcttgagcg cgaggatcgt gcccgcgaga ccgagcgtaa ccacgttggc gataatcatc
     7801 ggcagggagt cgagatagat tccgtaggcg agccagagag ctacgccgat gacgaagatg
     7861 atgtacatgc cgcgcgaaat tccgcgcgtg tcgcgcgagc ggatggtcat cacggcctgc
     7921 ggaatgaaag aagcgctggt gagcgctgcg gcggcgaagc cgagccattc gggattcatg
     7981 cgcggattgt cttagaaaat ggtggccggg gttgggtgag cgcggtgctt tagcgaacgg
     8041 gcgagcggag cgagccctgg gattcagaca cctggcacaa tctttcgcgc agcgaaaaat
     8101 ggtggccggg gtcggaatcg aaccaacgac acgcggattt tcaatccgct gctctaccaa
     8161 ctgagctacc cggccacggt tgggatttcc ctcgaagagg gccctcgatc gaggggcgcg
     8221 tattagaccg gccgtcccca tggccgtcaa gcaagcaagg cctcaaaccc ttgatttgcg
     8281 acgcattgac accatttcgg gccgaccatg aataccgcag ccaaactcgc caccaccccg
     8341 cgcgcagtca tccagacgct tcgcggccag cccgcctcgg acggcgcggg cgtcaagctc
     8401 actcgtgtca tcggtcagcc caggctgccc gatctcgacc cgttcctgct gctcgatgaa
     8461 ttcggcacgg acaaggccga ggactacatc gccggatttc cggagcaccc gcatcgtggc
     8521 ttcgaaacgg tcacgtacat gctcgacggc cgcatgcgcc acaaggacaa ccacggcaat
     8581 gaaggcgtgc tcgagccggg cgccgtgcag tggatgaccg cgggccgcgg aatcgtgcac
     8641 tcggaaatgc ccgagcagcg cgaaggccgc atgcgcggct tccagctgtg gatgaacctg
     8701 cccgcgaagg acaagatgac cgcgcctaac taccaggaat tcggtggcga caaactgccg
     8761 gtcgtggaac ggacaggcgt gagcgtcaag gtgatcgccg gttcgctcga tggcgccacc
     8821 ggccccgtgc ggcagccggc cacagacccg gtctacatgg acatccggct cgatgcgggc
     8881 gcggacttca cgctgccgct gccggtgggt cattcggctt tcgtctacgt atacgagggc
     8941 ggtgtgtcgg tgggcgtctc gcgcgaagcg gcgaccatcc acgcgcagga gctcgcggtg
     9001 ctgggcgacg gcagcgaggt gcgcctgcag ggtcgcgagg cccgctcgcg cgcaatcctg
     9061 gtggccggcc ggccgttgcg cgaaccggtg gcgcgttacg gcccgttcgt gatgaacacc
     9121 cgtgaagagc tccaccaggc gttcgcggac ttccaggccg ggaagttcta gtcgggtggt
     9181 gcggggaatc cttccccggc accactctaa ttcgggacgg cgcagactgc tgcgccgtcc
     9241 atgatctcgc ggctatgatc ggcgccatgc gatctcgcct tgcgtacctg ctcctgctgc
     9301 tgccgatctg gcagtcgcca gtattcgcgc agtgtgctcc cgcgccagat tcaccctatt
     9361 tcttccgcaa tctcgctgag caacgagcgg aagccaaact cactgaagat cgcg
//
LOCUS       DASQJS010000094        12896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3503695, whole genome shotgun sequence.
ACCESSION   DASQJS010000094 DASQJS010000000
VERSION     DASQJS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12896
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3503695"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /gene="mnmG"
                     /locus_tag="VEW08_03745"
     CDS             <1..1511
                     /gene="mnmG"
                     /locus_tag="VEW08_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004654475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HYJ39877.1"
                     /translation="IVENGAVCGVVTMTGIKFHARSVVLTVGTFLGGRIHVGLDNYSG
                     GRAGDPPSNKLAERLRALPFRTARLKTGTPPRIDGRTIDYSALATQHSDEPRPVFSYL
                     GRQSDHPAQLPCHITATTERTHQIIRAATDRSPMFTGKIEGVGPRYCPSVEDKVVRFA
                     DKTSHQIFIEPEGLNTHEVYPNGISTSLPYDVQFEFVRTIKGFENAHITRPGYAIEYD
                     FFDPRDLKASLETKALAGLYFAGQINGTTGYEEAAAQGLIAGINAARGAAGKEAFVPK
                     RSESYVGVLIDDLVTSGTREPYRMFTSRAEHRLLLREDNADLRLTPIGRDLGLVDDAR
                     WSFFEAKRDGSERELARFKVTRIKAAEIPAEWASRVLGETSTRDALSAFDLLRRPEVS
                     YEQVVELIGPMEKADDDRLDAQIRTQLEVAAKYSGYIERQEEEIARQRRNEETRLPAD
                     LDYARVSGLSNEVRQKLEQIRPATVGQAARVAGVTPAAVSILLVHLKKSRAA"
     gene            1511..3121
                     /locus_tag="VEW08_03750"
     CDS             1511..3121
                     /locus_tag="VEW08_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HYJ39878.1"
                     /translation="MGGVTRRDVLTAPLVALAVTSCGQRGEAPGKQSAILMRGNGPDP
                     DSLDPHKARSAEAMVVLRDLFECLTRLDKNAVTVPAAAESWTMSDDGRIYTFKLRPNL
                     RWSSGEPVVAEDFAAGFRRLVDPGTASQYADVIGVIVNASNIIAGRKPVDSLGVAAPD
                     AKTLVITLESPAVYLPGLMAHPSTAPLHRPSLAQFGDRFSRPGDQVSNGAFVLEEWLQ
                     GSYIRVNRNPHYWGNAANKIDGVKFVQLADENAELRAYRAGELHCTYVLPRGQFDWIR
                     ENLAAELHVTPQLSTYYYGFNLDRAQFAAHPKLRQALSMAVDRERLASSVLRMGELPA
                     YGWVPPGMYNYTAQSFEYAGMPVAERIEAARKLLAEEGYTRDKPLRFELRYNSGEVHS
                     KVAVAVASMWKEALGVEAQLAAVEFKSLLEDIDRRNMDMYRLSWVGDYNDPYTFLQYL
                     KGDFGINLPHYVSAAYDALLEEASRQTDASKRRSLLEQAERTMLADHPLIPLYFYVNK
                     HLVSPRLRGWYDNVMNVVYSQDLEIIPT"
     gene            3261..3569
                     /locus_tag="VEW08_03755"
     CDS             3261..3569
                     /locus_tag="VEW08_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39879.1"
                     /translation="MRHTEGSQGYAYNRGYAPTQFQDDVDVAECALDEQLEQLQRHYA
                     AAIRAVSRSRYEIELLEKRDDIPGYLLEHAIRQRAAAETRCVRLLSAIEALEDRLENA
                     "
     gene            complement(3592..5364)
                     /locus_tag="VEW08_03760"
     CDS             complement(3592..5364)
                     /locus_tag="VEW08_03760"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HYJ39880.1"
                     /translation="MRRKIVALLVPLVGLLTVDAALAAKKPANAPLLKLFENSWQEDL
                     ADDPIAATSLGDHRYDALLTNMSVEAIAARQQRAFARMAALRKIDKTKLEKADQLNYD
                     LFERETRILLNEAQFKPYMYATRSIDGPQLLAELAETHPFATAKDYDNWIARINASGV
                     YIDQWIVLLGQGATERRTQPRATVQKVLDQIKSSLVAEPEANPFYAPFKKMPASFDAA
                     TRDRLTAAAKTAITTVAVPAFQRFDKFFRDVYLPASRDTVGIYDTPDGDLYYKDRVKY
                     YTTIDNPDPTRIHNIGLEEVKRIRAEMEKTLEGINFLGTLDQFLSFIRNDPRFFYKTP
                     EELFAAYEKTARGIEPQLPKLFGKLPKGGFGIRPIPAGSAPTTTTAYYQPPSLDGARQ
                     GNYYVNLYKPESRPIWEIEALTAHESVPGHHLQIALSYELTGLPEFRRNADYTAFIEG
                     WALYSESLGYELGLYKDDFSKFGQLNYDMWRAVRLVVDTGMHQFKWTRDQAIYFFKQN
                     TGKSQQDIENEVDRYISWPGQALAYKLGQLRIQALRAEAEKALGDRFDIRAFHDQLLG
                     MGALPLGVLDTEMRSWIAAQSARR"
     gene            complement(5376..5864)
                     /locus_tag="VEW08_03765"
     CDS             complement(5376..5864)
                     /locus_tag="VEW08_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39881.1"
                     /translation="MSEAPVPEISVEPDHFDVVNSDVVLVARLESTFALCLYDAVLES
                     GALIHLRVAPPGRVQDPNLTDTTLSTDLLLLDRCFVELRKAEPRAQHWQAKVVGQVQD
                     LPGARERFDGIQSFLSAFLADANVKLVSCDTHVNHVQLLKFRPAMGHVRSEPLAAQRL
                     GS"
     gene            6067..7050
                     /locus_tag="VEW08_03770"
     CDS             6067..7050
                     /locus_tag="VEW08_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HYJ39882.1"
                     /translation="MLESGTIWVVLVVIGIIIVAIKTLVIVPQGFEYTRERLGKFDHA
                     MPPGPHWLIPFFDNVGQKINMMEQVLDVPRQEVITKDNAMVAIDAVVFYQVLDAPKAA
                     YEVANLRLAMVNLTMTNIRTVVGSMDLDETLSKRDEINHRLLKVVDDATTPWGVKCTR
                     IEIKDITPPANLIEAMGRQMKAEREKRANILDAEGFRQAAILKAEGEKQAVVLAAEGN
                     KEAAFREAEARERLAQAEARATAMVSQAIAAGDVQAINYFVAQKYVEALKEFANSPNQ
                     KVFMMPMESAGLLGALGGIVELAKDAMQRQPSSLPPGSPGAVRTPPRAPQS"
     gene            7047..7490
                     /locus_tag="VEW08_03775"
     CDS             7047..7490
                     /locus_tag="VEW08_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HYJ39883.1"
                     /translation="MSGFLQNLEWWHWMVLAVLLAAAETLVPGAVVIWFAASAAVIGL
                     LMVGIDIPWQYQLIGFGLLGMAALLAYRAYRKKNPGTFEQPNLNQRGLQYVGSELVLV
                     EPIEQGSGKARLGDGVWKVSGPELPAGARVRVTGVNGTILTVIPV"
     gene            7515..7943
                     /locus_tag="VEW08_03780"
     CDS             7515..7943
                     /locus_tag="VEW08_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008050583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYJ39884.1"
                     /translation="MLSVEQLLNQKPRAIHSIGPEEPVLAAIKKMAEHHIGALVVMSG
                     EKLVGIVSERDYARKVVLLGRSSTETRVDAIMSAKVVTVTAKQDAHDCMRLMTDMRIR
                     HLPVVAGDRVVGVLSIGDLVRAVIEEQERTIADLESYIRS"
     gene            7982..8668
                     /locus_tag="VEW08_03785"
     CDS             7982..8668
                     /locus_tag="VEW08_03785"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HYJ39885.1"
                     /translation="MLAKGVAPEQDGYAICPPMFSQAELHGLTAELAAAPLQRTRAGA
                     RHILGCSSIARLAKDSRLIDLASRWLGAAAVPFKATLFDKSPDANWLVAWHQDTALPI
                     SEHRDVAGWGPWSEKSGVTYAHAPAHVLEQVIALRVHLDDSTPDNGPLRVLPRTHTAG
                     VLSDAEIHELSLRVKPVSCPVPAGGIVVMRPLIIHASSKLNAPSPRRVLHLEYAASLA
                     LESGLELRVA"
     gene            complement(8675..10123)
                     /locus_tag="VEW08_03790"
     CDS             complement(8675..10123)
                     /locus_tag="VEW08_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HYJ39886.1"
                     /translation="MLAVAHLLGLMMAFFGLLYLLPIAWSLGVQDGAVMDFVIAAGIN
                     AVAGLAVAMATRRFRRELKPRDGFLLVSLAWVLMSASAAIPLMIALPDLSFTDAYFEA
                     MSGLTTTGSTVLNHLDTLPESINIWRHTLHWIGGIGIIVLVVAVLPLLGVGGMQLYKA
                     ETPGPVKDEKLTPRITETAKALWFTYLAISTAGVIGLRIAGMSWFDSICHGFSAIALG
                     GFSTHDVSVGYFNSAAVELVLIAIMVVAALNFSRHFLAFRSLSLRPYRNDSEGKAVLI
                     VLGSSVVLVSSLLWIDGTYPSMGEGLRHSVFNVVSIATTTGFVTEDYEKWPAFLPAWL
                     LFLSCITCSTGSTGGGIKMFRTLLLVRQARRELKLLVHPNAMIPIRIGGQAIPDRVAY
                     SVLAFIFLYFGTSLVLTFALLATGLDLVSALSAAVGSMNNLGPGMGAVGPSTNFSGLT
                     DAQTWICTLAMLIGRLEIFSVLVLFTATFWRK"
     gene            complement(10140..11513)
                     /gene="trkA"
                     /locus_tag="VEW08_03795"
     CDS             complement(10140..11513)
                     /gene="trkA"
                     /locus_tag="VEW08_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HYJ39887.1"
                     /translation="MKIVILGAGQVGRTAAYHLSREEANEVTVVDTNEEVLRDLQDRL
                     DIRTVAGNAAYPSVLEAAGGASADIVVALTSSDEVNMMACEVAYTLYRTRTKIARIRS
                     SEYTRHPKLFADEALSVDVWISPEQLVTEYIGELIKYPGALQVLDFADGRVRLVGIRA
                     RKGGLLVSQKLAKLKEHHPNTEARVVAIYRAGRSIIPDGETIVEEGDEVFFIAARDDI
                     RKFMSEIRKEEAPAKRVVIAGGGNIGLSLARKLEEQHHVKLIERDPKRARRISELLLN
                     TIVLNGDAADEELLIEENIDSCDVFASLTNSEEANILSAMLAKRLGARKVMALINKPS
                     YADLMESGSIDIAISPQTITIGSLLAHVRRGDVVRVHSLRRGAAEALEAVVHGTDKTS
                     RVVGRNVADIPLPEGAAIGAVVRGDAVMIAHHDTRIQNDDHVIVFLSDRRHVEQVERL
                     FSGPGGR"
     gene            11625..>12896
                     /locus_tag="VEW08_03800"
     CDS             11625..>12896
                     /locus_tag="VEW08_03800"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYJ39888.1"
                     /translation="MNTATPETPVAHEFRVRYSTGQPASSDQPLNNTFGLTGEGKLVV
                     DRGALVFEGTRSGFTLTGKPRIALADVANVDYNATNSAFLIRTRDGRHYIIVWLASRE
                     DAEALWAMLPQEKTPEFLADQAAHERFGKAMSVLGARSLVTPAIIAINVAVFIVMLIA
                     GADLMNPNPAVHIRFGSNFGPLTWTGEEWRLLTSAFLHFGLIHIALNMYALYQGGGLV
                     ERLFGSTRFAVIYLLSALSGSVVSGWWDPFRNSAGASGAIFGVYGALLAFLAVRRVDI
                     PPTMLKSISSSALLFCLYSLVIGAAHPLIDNACHIGGLLGGFVSGVILARPFTTEARA
                     TRQPARLLVAVLAIVLPLALLAQPLIAENGSKSAGLRFARDMETFVRAEAEIVRRQTD
                     ILTFPPNARVNRLEIAQRLRHEVLEPWRAASR"
BASE COUNT         2437 a         4046 c         4104 g         2309 t
ORIGIN      
        1 tcatcgtcga gaacggcgcg gtgtgtggcg tcgtcaccat gaccggcatc aaattccatg
       61 cgcgaagcgt ggtgctcacg gtcggcactt tcctcggcgg ccgcattcac gtcggtctcg
      121 acaactacag cggcgggcgg gccggcgatc caccgtctaa caaacttgcc gagcggcttc
      181 gcgcgctgcc gtttcgcacc gcacgcctga agaccggcac accgccgcgc atcgacggcc
      241 gcaccatcga ctactcggcg ctcgccacac agcattccga cgagccgcgc ccggtgttca
      301 gctatctcgg ccggcagagc gaccatcccg cgcagttgcc ctgccacatc acggccacca
      361 cggaacgcac gcaccagatc attcgcgccg cgaccgatcg ctcgccgatg ttcaccggca
      421 agatcgaagg cgtgggccct cgctactgcc cgagcgtcga agacaaggta gttcggttcg
      481 ccgacaagac ttcgcaccag atcttcatcg aacccgaagg gttgaacacg cacgaggtct
      541 acccgaacgg catctccacg agccttccgt atgacgtgca gttcgagttc gtgcgcacca
      601 tcaagggttt cgaaaacgcc cacatcacgc gtccgggcta cgccatcgaa tacgacttct
      661 tcgatccgcg tgatctcaag gcctcgctcg agaccaaggc gctcgccggc ctgtatttcg
      721 ccggccagat caatggcacc acgggttacg aagaagccgc ggcgcagggc ctcatcgcgg
      781 gaatcaacgc ggctcgcggg gctgctggca aagaagcctt cgtgccgaag cgcagcgaga
      841 gctacgtggg tgtgctcatc gatgacctgg tgacgagcgg cacgcgcgag ccgtatcgca
      901 tgttcaccag ccgcgccgaa catcggctgc tgttgcgtga agacaatgcc gatctgcgcc
      961 tcactcccat cggacgcgac cttgggttgg tcgacgatgc acgctggagc ttcttcgaag
     1021 ccaagcgtga tggcagcgag cgggaacttg ctcgcttcaa ggtaacgcgt atcaaagcgg
     1081 cagaaatacc cgcggaatgg gcgagccgcg tactgggcga aacctccacg cgagatgcgc
     1141 tgtccgcctt cgatctgctg cgccggccgg aggtgtcgta cgagcaggtg gtggaactca
     1201 tcggcccgat ggaaaaggcg gacgacgacc gcctggacgc gcagattcgc acgcagctcg
     1261 aagtcgccgc caaatactcg ggatacatcg aacgccagga agaagaaatc gctcgtcaac
     1321 ggcgcaacga ggaaacgcgc ctgcccgcgg acctcgacta cgcgcgtgtc agtggcctgt
     1381 cgaacgaagt ccggcagaag ctcgaacaga ttcgtccggc cacggtcggg caggcggcgc
     1441 gcgtcgccgg cgtcacgccc gcggcggtct ccatcctgct ggttcatctc aagaagtcgc
     1501 gcgcggcctg atgggcggag taacccgacg cgacgtcctc acggcgccac tcgtcgcgct
     1561 cgcggtcacc tcctgcggcc agcgcggtga ggcacccggc aagcaatccg caatcctgat
     1621 gcgcggcaat ggtccggatc cggactcgct cgatccgcac aaggcacgct cagccgaggc
     1681 gatggtggtg ctgcgcgatc tgttcgagtg cctcacgcgg ctcgacaaga atgccgtcac
     1741 ggttccggcg gccgccgaaa gctggacgat gagcgacgac ggccgcatct acacattcaa
     1801 gctgcgtccg aaccttcggt ggtccagtgg tgagccggtg gtggccgaag acttcgccgc
     1861 gggatttcgc aggctggtgg acccgggcac cgcatcgcag tacgcggacg tcatcggcgt
     1921 catcgtcaat gcctcgaaca tcattgcggg caggaagccg gtggactcgc tcggcgtcgc
     1981 cgcgcccgac gcgaagacgc tggtcatcac gctggaaagc cccgcggtct atctaccggg
     2041 gctcatggcg catccgtcca ccgcgccgct gcatcgcccc tcgctcgcgc aattcggtga
     2101 tcgtttttcg cgcccggggg accaggtgtc gaacggcgcg ttcgtgctcg aagaatggct
     2161 gcagggcagc tacattcgcg tgaaccgcaa cccgcactac tggggaaatg cggccaacaa
     2221 gatcgacggc gtcaagttcg tgcagctcgc ggatgaaaac gccgagctgc gcgcataccg
     2281 cgccggggaa ttgcattgca cttacgtgtt gccgcgcgga cagttcgact ggatcaggga
     2341 aaacctggcc gcggaactgc acgtgacgcc gcagctcagc acctattact acggcttcaa
     2401 tctcgatcgc gcgcaatttg ccgcacaccc gaagctgcgc caggcgctgt cgatggcggt
     2461 cgatcgcgaa cgccttgcga gctccgtgct gcgcatgggt gaacttcccg cgtatggctg
     2521 ggtgccgccc ggcatgtaca actacaccgc gcaatcattc gagtacgccg ggatgccggt
     2581 ggcagaacgc atcgaagccg cgcgcaagct gctggccgaa gagggataca cgcgcgacaa
     2641 gccgctgcgc ttcgagctgc gctacaacag cggcgaggtg cattccaagg tcgccgtggc
     2701 ggttgcgtcc atgtggaagg aggcgctggg cgtggaggcg caactcgcgg ccgtggaatt
     2761 caagtcgctg ctcgaggaca tcgaccgtcg caacatggac atgtatcggc tgtcctgggt
     2821 gggcgactac aacgatccgt acacctttct gcagtacctg aagggtgact tcggtatcaa
     2881 tctcccgcat tacgtcagcg ctgcctacga tgcgctgctc gaagaagcat cgcggcagac
     2941 cgatgcatcg aaacgccgct cgctgctcga acaggctgaa cgcaccatgc tggcggatca
     3001 cccgctgatt ccgctgtatt tctacgtgaa caagcacctg gtcagcccca ggctcagggg
     3061 ctggtacgac aacgtcatga acgtcgtcta ttcccaggac ctcgaaatca ttcccacgta
     3121 gacgtcaggc atcgtctgac gagccggcgg gactcttcta cagcccgatg agtcgggtca
     3181 ccgacattca acagcaatag attttcgtga tgatggcctc accccggtac acacattcgg
     3241 ccgggggaag aggagcggtc gtgagacata cagaaggcag tcagggctac gcgtacaaca
     3301 gaggctacgc ccccacgcag ttccaggatg acgtcgatgt ggcggagtgc gcactcgacg
     3361 aacaactgga gcaattacag cgtcactacg cggcagccat ccgcgccgtt tcccgctcgc
     3421 gttacgagat cgaacttctc gagaagcgcg acgacattcc cggatacctc ctcgaacacg
     3481 ccatcaggca gcgcgccgca gcggaaaccc gctgcgtacg cctgctcagc gcgatcgaag
     3541 ctctcgaaga ccgtttggag aatgcgtgat gcgccgacga acccttgacg attaccgccg
     3601 cgcgctttgc gccgcgatcc aggaacgcat ttcggtgtcc agcacgccca ggggcaatgc
     3661 gcccattccg aggagctggt cgtggaatgc gcggatatcg aagcgatcac cgagcgcctt
     3721 ttcggcttcg gcgcgcagcg cctggatcct gagctgacct aacttgtacg ccagcgcctg
     3781 gccgggccag gaaatgtagc ggtcgacttc gttctcgatg tcctgctggc tcttgccggt
     3841 gttctgcttg aagaagtaga tagcctgatc gcgcgtccac ttgaactggt gcatgccggt
     3901 gtcgaccacc aggcgaacgg cgcgccacat gtcgtagttg agctggccga acttcgagaa
     3961 gtcatccttg tacagcccga gctcgtagcc gaggctttcg gaatacaggg cccagccttc
     4021 gatgaacgcg gtgtaatcgg cgttgcgccg gaattccggc agcccggtga gctcgtacga
     4081 caacgcgatc tgcagatgat gtcccggcac ggactcgtgc gcggtcagcg cttcgatctc
     4141 ccagatcggc cgcgactccg gcttgtacag gttcacgtag tagttgccct gtcgtgcgcc
     4201 gtcgagcgac ggcggctggt aatacgccgt ggtcgtcgtc ggcgcgctgc ccgcgggaat
     4261 ggggcggatg ccgaagccgc ccttcggcag tttgccgaac agtttcggca gctgcggctc
     4321 gatgccacgg gcggttttct cataggccgc gaacaactct tcgggcgtct tgtagaagaa
     4381 gcgcggatcg ttgcggatga aggacaggaa ctgatcgagc gtgcccagga aattgatgcc
     4441 ctcgagcgtc ttttccatct ccgcgcgaat ccgcttcact tcctcgaggc cgatgttgtg
     4501 aatgcgggtg ggatccgggt tgtcgatggt cgtgtagtac ttgacccggt ccttgtaata
     4561 caggtcgccg tccggcgtgt cgtagatgcc caccgtgtca cgcgatgcgg gtagatagac
     4621 atcgcggaag aacttgtcga agcgctggaa cgccggcacc gccacggtcg tgatcgcggt
     4681 cttcgcggcg gccgtcaggc ggtccctggt ggcggcatcg aaactcgccg gcatcttctt
     4741 gaacggcgcg tagaacgggt tcgcttcggg ctcggcgacg agcgacgact tgatctggtc
     4801 gagcaccttc tgcacggtgg cgcgcggctg cgtgcgccgc tcggtcgcgc cctggcctaa
     4861 caacacgatc cactggtcga tgtacacgcc gctcgcgttg atgcgcgcga tccagttgtc
     4921 atagtccttg gccgtcgcga acggatgggt ctcggcgagt tccgccagca gctgcggtcc
     4981 gtcgatggag cgcgtcgcgt acatgtaagg cttgaactgc gcttcgttga gcagaatgcg
     5041 ggtttcgcgc tcgaagaggt cgtagttgag ctggtcggcc ttctcgagtt tggtcttgtc
     5101 gatcttgcgc agtgcggcca tgcgcgcgaa tgcgcgctgc tggcgcgccg cgatcgcctc
     5161 caccgacatg ttggtcagca gcgcgtcgta gcgatgatcg ccaagcgacg ttgcggcgat
     5221 ggggtcgtcg gccaggtcct cctgccagct gttttcgaac aacttgagca gcggagcgtt
     5281 ggcaggcttc ttggcggcca gcgcggcatc gacggtgagc aaccccacga gggggaccag
     5341 caaggcaact atttttcggc gcatggcgcc gattttcagc taccgaggcg ctgagcggca
     5401 aggggctcgc tgcggacgtg acccattgcg ggacggaatt tgagcaattg cacgtggttc
     5461 acgtgggtat cgcaggaaac cagcttcacg ttggcgtcgg cgagaaacgc ggagagaaaa
     5521 ctctggattc catcgaagcg ttcgcgggct cccggcaggt cctggacctg gcctacgact
     5581 ttagcctgcc agtgctgcgc tcgcggctcg gccttgcgca gctcgacgaa gcagcgatcg
     5641 agcaacaaca ggtcggtaga cagcgtggtg tcggtgagat tggggtcctg cacgcggccc
     5701 ggcggcgcca cgcgcaggtg aatgagcgca ccggattcga gcaccgcgtc atacagacat
     5761 aaggcgaacg tggattcgag gcgcgcgacg agcacgacat cgctgttaac gacgtcgaaa
     5821 tgatcgggtt caacgctgat ttccggtacg ggggcttctg acacggcgac ctgaaacttg
     5881 agactgttat ccccggggca cgtgcgtcag cttctggcgg ctggcgtctc gctggcttcc
     5941 ctcacggcgg atcatcgcag agtcagcgcc gcattcccat gtcgcagggc ggctacgtgc
     6001 ggaaaaggcg accctaagcg tatgattccg gggcgtcgag tgcgggaaca tcagagggga
     6061 acacacatgc ttgagtccgg caccatctgg gtcgttcttg tcgtcatcgg catcatcatc
     6121 gttgcgatca agacactggt catcgtgccc cagggtttcg agtacacccg cgagcgcctg
     6181 ggcaaattcg accatgccat gccgcccggg ccgcactggc tcattccctt cttcgacaac
     6241 gtcggccaga agatcaacat gatggagcag gtgctcgacg tcccccgcca ggaggtcatc
     6301 accaaggaca acgccatggt cgccatcgac gccgtggtgt tctaccaggt gctggacgcc
     6361 ccgaaagcgg catatgaagt agccaacctg cggctcgcca tggtgaacct gaccatgacg
     6421 aacattcgca cggtggtcgg ctccatggac ctcgacgaaa cactgtccaa gcgcgacgaa
     6481 atcaaccatc gcctgctcaa ggtggtcgac gacgcgacga cgccgtgggg cgtgaaatgc
     6541 acgcgcatcg aaatcaagga catcacgccg ccggcgaatc tcatcgaggc gatgggccgc
     6601 cagatgaaag ccgagcgcga gaagcgcgcc aacatcctcg acgccgaagg cttccgccag
     6661 gccgccatcc tcaaggccga gggtgaaaag caggctgtgg tgctcgcagc cgaaggcaac
     6721 aaggaagctg cgttccgcga ggcggaagcg cgtgagcgcc tggcgcaggc cgaagcgcgc
     6781 gccacggcca tggtgtcgca ggcaatcgcg gctggcgacg tgcaggccat caactacttc
     6841 gtggcacaga aatacgtcga ggcactgaag gaattcgcga attctccgaa tcagaaagta
     6901 ttcatgatgc cgatggagtc cgccgggttg ttaggcgctc tgggtggcat cgttgaactc
     6961 gccaaggacg ccatgcagcg gcaaccgagc agcctgccac caggttcgcc gggcgccgtg
     7021 cgcacacccc cgcgagcgcc gcagtcatga gcggattcct ccagaacctc gagtggtggc
     7081 actggatggt gctggcggtg ttgctggccg cagcagaaac actggttccg ggcgcagtcg
     7141 tcatatggtt cgcggcctcc gcagccgtga tcggcctgct gatggtcggc atcgatattc
     7201 cctggcaata ccagctcatc ggttttggcc tgctgggcat ggctgcattg ctggcgtatc
     7261 gcgcctatcg caagaagaat cccggcacat tcgaacaacc caacctcaat cagcgcggac
     7321 tgcagtacgt tggcagcgag ctggtgctgg tcgagccgat cgagcaggga tcgggcaagg
     7381 cgaggctcgg tgatggcgta tggaaagttt ccgggcccga gctgcccgcc ggcgcgcgcg
     7441 tgcgcgtcac gggcgtcaac ggcacgattc ttacggtgat tcctgtctga gatgttcgag
     7501 atacggaggt cgcgatgctt tccgtcgagc aactgctgaa tcagaaaccc agggctatcc
     7561 actcgatagg tcccgaggag cccgtgctcg ccgccatcaa gaagatggcc gagcatcaca
     7621 ttggtgcgct ggtcgtgatg tccggcgaaa agctcgtggg catcgtctcc gagcgtgact
     7681 acgcgcgcaa agtggtgttg ttaggccgct cttcgactga aacgcgcgtc gacgccatca
     7741 tgagcgccaa ggtggtcacg gtcaccgcga agcaggatgc gcacgactgc atgcgcctca
     7801 tgaccgacat gcgcatccgg catctgccgg tggtggcggg agatcgcgtg gtcggcgtgc
     7861 tgtccatcgg cgacctggtg cgcgcggtca tcgaagaaca ggagcgaacg attgcggatc
     7921 tcgagagcta catccgcagc tgaaatgggg acaggccccg caaagcaggg ccgttcctga
     7981 tttgcttgca aaaggggtcg cgcctgaaca agacgggtac gcgatctgcc cgccgatgtt
     8041 ttcgcaggcg gaattgcacg ggctcacggc ggagctcgcc gccgcgccat tgcagcgaac
     8101 gcgtgccgga gcccgtcaca tccttggatg cagcagcatt gcccggctgg cgaaggattc
     8161 acggctcatc gacctcgcgt cccggtggtt aggcgcggcg gccgtgccgt tcaaggcgac
     8221 cctgttcgac aagagtcccg atgcgaactg gctggtggcg tggcaccagg acacggcact
     8281 gccgatatcc gaacaccgcg acgtcgccgg ctggggccct tggtccgaga aatccggcgt
     8341 gacctatgcc cacgctcctg cccacgtgct ggaacaggtg attgcgctgc gcgttcacct
     8401 cgacgactca acccccgaca acggaccgct gcgcgtactt ccccggacgc acacggcggg
     8461 tgtgctgagt gacgcggaaa tacacgagct ctcgcttcga gtgaagccgg tgtcatgccc
     8521 cgtacccgct ggcggcattg tcgtcatgcg gccactgatc atccacgctt cttcaaaact
     8581 caacgcgccg tcgccgcggc gcgtgctgca tctcgagtac gcggcgtcac tcgcgctcga
     8641 atcgggcctc gagcttcgcg tcgcatgagc ccatctactt ccgccagaac gtggcggtga
     8701 acaacaccag cacgctgaag atttccaggc ggccgatgag catcgccagt gtacaaatcc
     8761 acgtttgcgc gtcggtcagg cccgagaagt tcgtcgaggg gcccaccgcg cccataccgg
     8821 ggcccagatt gttcatggaa cctaccgccg cggaaagcgc cgacacgaga tcgagtcccg
     8881 tcgccagcag cgcaaaagta agcaccaggc tggtcccgaa atatagaaag atgaaagcta
     8941 gtactgaata cgcaacccgg tcggggatcg cctggccgcc aatcctgatg ggaatcatgg
     9001 cgttcgggtg cactagcaat ttgagctcgc ggcgcgcctg ccgcaccaaa agcagggtgc
     9061 ggaacatctt gatgccgccg ccggtggagc cggtactgca tgtgatgcaa ctcaggaaca
     9121 gcaaccaggc gggcaggaac gccggccact tctcgtagtc ttcagtgacg aaaccggtgg
     9181 tggtggcgat ggacacgacg ttgaaaacgg agtggcgcag gccttccccc attgagggat
     9241 aggtgccgtc gatccacagc agcgaggaga ccagcactac ggaggagccg agcacgatca
     9301 gcacagcctt gccctccgaa tcgttgcggt acgggcgcaa cgacagactc cggaatgcaa
     9361 ggaagtgccg cgaaaaattc agcgcggcca ccaccatgat tgcgatgagc accagttcca
     9421 cggcggcgga attgaaatac ccaacgctca catcgtgagt ggagaagccg ccgagcgcga
     9481 tcgcagaaaa cccgtgacaa atcgagtcga accagctcat gcccgcgatg cgcagaccaa
     9541 tgacacccgc ggtgctgatg gcgaggtacg tgaaccacag cgctttcgcc gtctcggtga
     9601 tgcgcggcgt cagcttctcg tccttgaccg ggccgggagt ctccgccttg tagagctgca
     9661 tgccaccaac gcctaacaaa ggcagcactg ccaccaccag gacgatgatg ccgatgccgc
     9721 ctatccagtg cagagtgtgg cgccagatat tgattgattc cggaagtgtg tcgagatggt
     9781 tcagcaccgt ggatcccgtg gtggtgagac ccgacatcgc ctcaaaatat gcgtcggtga
     9841 agctcagatc cggcagagcg atcatgagcg gaatggcggc ggacgcggac atcagcaccc
     9901 acgccaggct gaccaggagg aagccgtcgc gcggcttgag ttcacgacga aaccggcgcg
     9961 tggccatcgc cacggcgagg cccgcaacgg cattgatgcc ggcggcgatc acgaagtcca
    10021 tgaccgcacc gtcctgcacg cccagcgacc aggcaatggg caatagatag agcaggccga
    10081 agaaggccat catgagcccg agcaggtggg cgacggctaa caagatctac gcctcgcctt
    10141 cagcgcccgc cgggcccgga gaacaggcgc tcgacctgct cgacgtgacg ccggtcggaa
    10201 aggaacacga tcacgtgatc gtcgttctga atcctggtgt cgtgatgcgc gatcatcacc
    10261 gcatcgccac gcaccaccgc gccgatggcc gcgccttcgg gcaacgggat atcggcaacg
    10321 ttccggccca ccacgcgcga ggtcttgtcg gtgccgtgca ccacggcctc gagtgcttcg
    10381 gcggcgccgc ggcgcaggga atgcacgcgc accacgtcac cgcggcgcac gtgcgccagc
    10441 agcgaaccga tggtgatggt ctgcggggaa atggcgatgt cgatgctgcc gctttccatg
    10501 agatcggcgt acgaaggctt gttgatgaga gccatcacct ttcttgcgcc caggcgcttg
    10561 gccagcatgg ccgaaaggat gttcgcctct tcggagttgg tgagtgaagc gaacacgtcg
    10621 cagctgtcga tgttttcttc gatcagcagc tcttcgtcgg cggcgtcgcc gtttagcacg
    10681 atggtgttca gcaacagctc ggagatgcga cgcgcgcgct tcggatcgcg ttcgatgagc
    10741 ttcacgtggt gctgttcctc gagcttgcgc gccagcgaca ggccgatgtt gccgccgccg
    10801 gcgatcacca cgcgcttggc cggcgcttct tccttgcgga tctcgctcat gaacttgcgg
    10861 atgtcgtcgc gcgccgcgat gaagaacacc tcgtcgcctt cctcgacgat ggtctcccca
    10921 tcgggaatga tgctgcggcc ggcacggtag atagccacca cgcgcgcttc ggtgttcggg
    10981 tgatgctcct tgagtttcgc cagcttctgg ctgaccaaca ggccgccctt gcgcgcgcga
    11041 atgcccacga gccgtacgcg gccgtcggcg aagtccagca cttgcagcgc tccgggatac
    11101 ttgatgagct cgccgatgta ctcggtgacg agctgctcgg ggctgatcca cacatccacc
    11161 gacagcgcct cgtcggcaaa caacttcgga tggcgcgtgt attcggacga acgtatgcgg
    11221 gcgatcttcg tgcgagtgcg gtacagcgtg tacgccacct cgcaggccat catgttgacc
    11281 tcgtcgctcg aggtcagcgc caccacaatg tcggctgagg cgccgccggc ggcctcgagc
    11341 accgatggat aggcggcgtt gccggctacc gtgcggatgt cgaggcgatc ctgcagatcg
    11401 cgcaacactt cttcattggt gtcgacgacc gtgacttcat tggcctcttc acgcgacagg
    11461 tggtaggccg cagtgcgccc gacttggccg gcgccgagta tcacgatctt catgggggcg
    11521 atattgtacg gtgccgggcc agattggagt ccggcaattc gacaaagtgg gttcggtggc
    11581 cgggtggcga gttttcccgg tttcccggcc cggcacccaa ctctatgaac acagccactc
    11641 ccgaaacgcc agttgcccac gaattccgcg tgcgttacag cacgggtcag ccggcgagct
    11701 ctgaccagcc gctcaacaac accttcgggc tcacgggcga gggcaagctc gtggtggatc
    11761 gcggagcgct cgttttcgag ggtacgcgca gtgggtttac gctgaccggc aagccacgca
    11821 tcgcgctcgc cgacgttgcc aatgtggact acaacgccac caacagcgcc ttcctgatcc
    11881 gcacgcgcga cgggcggcac tacatcatcg tgtggctggc ctcgcgcgaa gacgccgaag
    11941 ccctctgggc aatgcttccg caggaaaaga cgccggagtt cctggcggac caggcggcgc
    12001 acgagcgttt cggcaaggcc atgtcggtac tcggcgcgcg ttcgctggtt acgcccgcaa
    12061 tcatcgccat caacgtcgcg gtcttcatcg tgatgttgat tgccggtgcg gatctcatga
    12121 atcccaaccc cgccgtgcac atccggttcg gctcgaactt cggtccgttg acgtggacgg
    12181 gtgaggaatg gcgactgctg acgtcggcat tcctgcactt cggactcatt catatcgcgc
    12241 tgaacatgta cgcgctgtac cagggtggcg gcctcgtgga gaggttgttc ggcagtactc
    12301 ggtttgccgt gatctatctg ctctcggccc tgtcgggcag cgtggtgagc ggctggtggg
    12361 atccgtttcg caacagcgcc ggcgcgtcgg gcgcgatttt cggcgtgtat ggcgcgctgc
    12421 tggcattcct cgccgtgcgc cgcgtcgaca ttccgcccac catgctgaag agcatcagca
    12481 gcagcgcact gctgttctgc ctgtactcgc tggtgatcgg cgccgcgcat ccgctcatcg
    12541 acaatgcctg ccacatcggc gggttgttag gcgggttcgt ctcgggcgtt atcctggcac
    12601 ggccgttcac gacggaggcg cgcgccacgc ggcagccggc caggctgctc gtggccgtat
    12661 tggccattgt ccttcccctc gcgttactgg cccagccgtt gatcgccgag aacggcagca
    12721 agtccgccgg tctgcggttc gcgcgcgaca tggaaacctt cgtccgcgcg gaagcggaga
    12781 tcgtgcgtcg ccagaccgac atcctcacgt ttccacccaa cgcacgcgtg aatcggctgg
    12841 agattgccca gcgactgcgc cacgaagtgc tcgagccctg gcgcgccgcg tcgcgg
//
LOCUS       DASQJS010000095         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3821440, whole genome shotgun sequence.
ACCESSION   DASQJS010000095 DASQJS010000000
VERSION     DASQJS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3821440"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VEW08_03805"
     CDS             complement(<1..567)
                     /locus_tag="VEW08_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39889.1"
                     /translation="MYRASCLHVFLLALASSAALAAKPQFEARDCSTDASAAGARCGV
                     VYVPENHDKPRGRKIPIHVVVLPATGPHDGKRAQFDLEGGPGFAATDFLEFYAGEGAP
                     YRQKRDIVLADMRGTGQSNPLRCPGLEKRQPALPLYPPELVAECAKQLSSYGTTLALR
                     YMADFPKAVHAAVLMGTVPASRMPPRYHA"
     gene            602..2275
                     /locus_tag="VEW08_03810"
     CDS             602..2275
                     /locus_tag="VEW08_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019407031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HYJ39890.1"
                     /translation="MDTFKQFGRRRFVQGLAGGALLLAHSRGRALAAGSTPAELVGPD
                     FHLTIDELPVNYTGAARRAVAVNGQVPAPLLRMRQGDTVTIRVTNNLREATSIHWHGF
                     ILPADMDGVPGLSFDGIAPGTTFTYRFKVNQSGTYWYHSHSRFQEQIGLYGPIIVERR
                     GGERHGADREHTLLLSDWTDHDPEHIYATLKRQSDYYNFGKRTFSDFLADARKQGLSA
                     AAADRRMWGAMRMNPTDLADVTGYAYTYLLNGATPAANWTGLFTPGERVRLRLINGSS
                     MSFFDVRIPGLKLTVVATDGQDVEPVTVDELRIGTAEVYDVIVTPGDAAYTLFAQSMD
                     RSGYARGTLAPRAGMTADVPTMDTRALLTMSDMGMQHAGMDHSKMDHSAMPKVVHAPA
                     ETGPIVDMRADMPNTRLDDPGVGLRNNGRRVLTYADLATLGGPIDRRVAAREIELHLT
                     GHMERYIWSFNGQKFSDAEPLRFAHNERLRIALVNDTMMTHPIHLHGMWSEITDASGK
                     FVARKHTVTVQPGQRLTCEVTADALGSWAYHCHLLYHMEAGMFREVVVA"
     gene            2272..>2571
                     /locus_tag="VEW08_03815"
     CDS             2272..>2571
                     /locus_tag="VEW08_03815"
                     /inference="COORDINATES: protein motif:HMM:NF017119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HYJ39891.1"
                     /translation="MRRLASAGVLLVAAAGTFAQSEHVPPDPPQTHVHEMPYREMVEM
                     MGMDDRALFGKVMLDRLEWRDADVSTYEWEAAAWYGGDFNKVWLETEGEHVDGSTS"
BASE COUNT          465 a          843 c          839 g          424 t
ORIGIN      
        1 tgcgtgataa cgcggcggca tgcggctggc gggaacggtg cccatgagca ccgcggcgtg
       61 cacggccttc ggaaagtccg ccatgtagcg caatgcgagc gtggtgccgt aggaactcag
      121 ctgcttagcg cattcggcta cgagctccgg cggatacagc ggcaaggccg gctggcgttt
      181 ctcgaggcct gggcaccgca gagggttcga ttggcccgtg cctcgcatgt cggcgagcac
      241 gatgtcgcgc ttctggcgat atggcgcgcc ctccccggcg tagaactcga ggaaatccgt
      301 agcggcgaac ccggggccac cttcgaggtc aaactgagcg cgcttgccgt cgtgcggccc
      361 cgttgccgga agcactacga catggatcgg gatttttcgg ccgcgcggtt tgtcgtggtt
      421 ttccggaaca tacacgacac cgcagcgtgc gccggccgcc gacgcgtcgg tcgagcagtc
      481 tctcgcttca aactgcggtt tcgccgcgag cgccgcggac gacgccaacg ccagcaggaa
      541 aacgtgtaaa cacgaggctc ggtacatggc gcacgagtct gccagaattg acgctcaacc
      601 tatggatacc ttcaaacaat tcggccggcg gagattcgtg cagggcctgg ccggcggcgc
      661 gttgctgctc gcgcacagtc gcggccgcgc gctggcagcc ggcagcactc ccgcggaact
      721 cgttgggccg gactttcatc tcaccatcga cgaactgccg gtgaactaca ccggcgccgc
      781 acgccgcgcg gtggcggtca atggccaggt gccggcgccg ctgctgcgaa tgcgccaggg
      841 cgacaccgtc accattcgcg tcactaacaa cctgcgcgag gccacttcga tccactggca
      901 cgggttcatc ctgccggcgg acatggacgg agtgccgggc ctgtcgttcg atggcatcgc
      961 gcctggcacc acgttcacct atcgcttcaa ggtcaaccag tccggaacct attggtatca
     1021 ctcgcattcg cgcttccagg agcagatcgg cctgtacggg cccatcatcg tcgagcggcg
     1081 cggcggcgag cgccacggcg ccgaccgcga acacacactg ctgctgtcgg actggaccga
     1141 ccacgacccc gaacacatct acgccacgct caagcgccag agcgactact acaacttcgg
     1201 caagcgcacg ttcagcgact tcctcgccga tgcgcgcaaa cagggcctct ccgccgccgc
     1261 cgcggaccgg cgcatgtggg gcgcgatgcg catgaacccc accgatctcg ccgacgtcac
     1321 cggctacgcc tatacctatc tactgaacgg ggccactccc gcggccaact ggaccggcct
     1381 cttcacgccg ggcgaacgcg tgcggctgcg gctcatcaac ggctcgtcga tgagtttctt
     1441 cgacgtgcgc attccggggc tgaaactcac ggtggtcgcc accgatgggc aggacgtgga
     1501 gccggtaaca gtcgatgagc tgcgcatcgg caccgccgag gtatacgacg tcatcgtcac
     1561 gcccggcgac gcggcctaca cgctcttcgc gcagtccatg gaccggtccg gctacgcgcg
     1621 cggaacgctg gcgccgcgtg ccggcatgac cgccgacgtt ccgacgatgg atacgcgcgc
     1681 gttgttgacg atgtccgaca tgggcatgca gcacgctggc atggatcaca gcaaaatgga
     1741 ccattcggcc atgccaaagg tggttcatgc tcctgcggaa actggaccaa tcgtcgacat
     1801 gcgtgccgac atgccgaaca cccggctcga tgacccgggc gtggggctgc gcaacaatgg
     1861 ccggcgtgtg ctcacgtatg cggacctcgc gacgctcggc ggccccatcg accgccgcgt
     1921 ggcggcccgc gaaatcgagc tgcatctgac cggacacatg gagcgttaca tctggtcgtt
     1981 caacggccag aaattttcgg acgccgaacc gctgcgattt gcgcacaacg aacgcctgcg
     2041 tatcgctctc gtgaacgaca ccatgatgac gcatcccatc cacctgcatg gcatgtggag
     2101 cgagatcacg gatgccagtg gcaagttcgt ggcccgcaag cacacggtga ctgtgcaacc
     2161 agggcagcgg ctcacctgcg aagtcacggc cgacgcgctg ggtagctggg cgtatcactg
     2221 ccacctgctc tatcacatgg aagcgggcat gttccgcgaa gtggtggtgg cgtgaggcgc
     2281 ctcgcgagcg cgggcgtgct gctcgtcgcc gccgccggta cgtttgcgca gagcgaacac
     2341 gtgccgcccg atccgccgca gacgcatgtg cacgagatgc cctaccgcga gatggtggag
     2401 atgatgggca tggacgatcg cgcgttgttc ggcaaggtca tgctggaccg gctcgagtgg
     2461 cgcgacgcgg acgtgtcgac ttatgagtgg gaggccgcgg cctggtatgg cggcgacttc
     2521 aacaaggtgt ggctcgagac cgaaggcgaa catgtggatg ggtcgacgag c
//
LOCUS       DASQJS010000096         8962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3504203, whole genome shotgun sequence.
ACCESSION   DASQJS010000096 DASQJS010000000
VERSION     DASQJS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3504203"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VEW08_03820"
     CDS             <1..459
                     /locus_tag="VEW08_03820"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HYJ39892.1"
                     /translation="VYWLLFLIFRFLLRRDVGAVGIADILLLVIVADAAQNAMSGGYS
                     TFAEGAILVLTIACWNWALDVLSYRFSAVRRFTTPARLALIKQGVLQRRNLRREYITV
                     EELHEKLREQGIEKVAEVKAAYLEGDGQISVIRNSGSQNSQAPERALPGA"
     gene            682..1569
                     /locus_tag="VEW08_03825"
     CDS             682..1569
                     /locus_tag="VEW08_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HYJ39893.1"
                     /translation="MGHEEKVVQWFNAVDWIQAGSRIAAILVVTFLLVFVARRLISGL
                     EKFTATHAHDVEAQKRAATVSNVLRKLATILITVIALLAVFNTLGISILPFLATAGVA
                     GIAIAFGAQSLVKDFFRGFFLLAENQIRQGDVVEVAGKSGLVEDLTLRYVQLRDYEGN
                     VHYVPNGEIGVVTSSSRTFAYAVVDVTIALDQDIAPVIDLMRETGMQLRANSEFGPRI
                     LEDLDIAGIEAWNDRGITLRARFKVRALEQSNVKREYLLRLKHAFDKADIQQPRSTVT
                     FVDRGGPATRAPVPEGPPA"
     gene            complement(1628..1852)
                     /locus_tag="VEW08_03830"
     CDS             complement(1628..1852)
                     /locus_tag="VEW08_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39894.1"
                     /translation="MTSAENSMLTMKIIKVLGIAGIAILAAAFFAARLQRRDEEQRRL
                     LNERLEKERWESEGGASRSGPATQSAVTAH"
     gene            complement(1849..2508)
                     /locus_tag="VEW08_03835"
     CDS             complement(1849..2508)
                     /locus_tag="VEW08_03835"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HYJ39895.1"
                     /translation="MKRAPLPLIAVISILAAPVWAISLPAAGPAQPLPEQALPSAALA
                     PGETLAPGSASLVPLMAADMQANQTTTVDAPTTGGTEVGKPIDDMTFVRKATESGYKE
                     VNSAKEALPQLQQPELKRIAEMLVNDHTGANARLSQLAGAKHWPVPAPQAQGAPPSGT
                     ASGDFDSKWTAEMIAGHERSVAMYRAQAKAGEDQDLRKFARETLPTIERHLAELKRLQ
                     K"
     gene            complement(2642..3061)
                     /locus_tag="VEW08_03840"
     CDS             complement(2642..3061)
                     /locus_tag="VEW08_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39896.1"
                     /translation="MTKTLDKQGRGSWTLAVAAAVGMCALAGCNQRESANETATDVAE
                     ERQDAQANVAEERREAADVAQEGSADRAAAEYDVAVAQADGQRKVAKEKCETLSSDAQ
                     KACKDQAEAMYESAKAQAQATLDAAKQAGSAEPAPTN"
     gene            3365..3706
                     /locus_tag="VEW08_03845"
     CDS             3365..3706
                     /locus_tag="VEW08_03845"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39897.1"
                     /translation="MTAQAKNFENGARNAESTVIELNRIVAQAEELLRTLGEEGGAAV
                     EAVRQRVLRTVSQAKVKLADVSTRARGAANEAAEVTDRYVHTNPWKSIAYGAAAGAAV
                     ALIAAALVRRD"
     gene            3779..4315
                     /locus_tag="VEW08_03850"
     CDS             3779..4315
                     /locus_tag="VEW08_03850"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HYJ39898.1"
                     /translation="MYATVALVLLFATDPQQELKHDVPQPRQQGTPTDPLFDKAYVAT
                     DEPAFVLSVIESTRQGEVDARLAAEELGKPELRDAAKRIGRLNGSTRARLEVLAKSKG
                     WRLPEKNPDRASTLPAGGDSRTGANFIVQQISFHESTVAQFRAQLNGSGDQDLKRELR
                     EALPGYEQNLELLLRLKL"
     gene            4439..5011
                     /locus_tag="VEW08_03855"
     CDS             4439..5011
                     /locus_tag="VEW08_03855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ39899.1"
                     /translation="MHRTDLAPFHARFPQGAAHTWLTRRWYYTPRSHRAYSVRQISPG
                     DRRLLAEFAMTLSQNSTDRELPCVQDLSDMMFERVIAGGVDNAVGFAALENTAGGDRV
                     IGVAAYAPASDESASFSLAVAHTHREEQVGRILLSTLVRHAKRVCVPRLTGEMHWSNR
                     PMQMLALSMGFSVEPEPRDRNLRRLVLPLK"
     gene            5038..6291
                     /locus_tag="VEW08_03860"
     CDS             5038..6291
                     /locus_tag="VEW08_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYJ39900.1"
                     /translation="MSESSSGATLQTGTREIEQVRKGGRVLIIVENLPCPFDRRVWQE
                     ARTLAAAGYVVSIICPKAPGYEKSFEQIDDIDIHRHALPAEADGVLGYALEYSVALAM
                     EFWLSLKILFGRGFDVIHACNPPDTIFLIGAFYKLFGKKYVFDHHDINPELYEAKFGK
                     RGFGRRLLVALERMSFNTADMVISTNESYRSIAIERGGKNPKDVYVVRSGPDLSRMKV
                     VPANAALKHGRKYLVGYVGVMGKQEGIDLLLQAVQLIVKQLLRTDIHFGLVGGGTELP
                     AMRELAKQLGVSEYVTFTGRAPDAELLEMLNTADICVNPDRANDMNDRSTMNKIMEYM
                     ALGKPVVQFDLTEGRVSAGEASWYARPNDVADLASKMVALLGDENQRIHMGAIGRERV
                     ERELSWQHEAPRLLAAYEQLTGDRS"
     gene            6288..6842
                     /locus_tag="VEW08_03865"
     CDS             6288..6842
                     /locus_tag="VEW08_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HYJ39901.1"
                     /translation="MKKFLLACCIIMALAWATGAMAQDANYTVKPGDLLSISVWKEPD
                     LQRPALVRPDGSFSFPLVGEVDAKGKTVADLNKAVSLKLAKYISDPVVTVSIQEIKGN
                     KVYVIGQVNKPGEFIMNPTVDVMQALSMAGGTTPFAALGDITVLRRTPTGKQSLPFRY
                     NDIIRGKRLDQNIDLQSGDVVVVP"
     gene            6855..8054
                     /locus_tag="VEW08_03870"
     CDS             6855..8054
                     /locus_tag="VEW08_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39902.1"
                     /translation="MKHSTLVMAVAATLASAQAGAAPWEWNPKVEAGYLYDDNYRLAT
                     PGSEIDVQGPMVDAELELRTLTQQGEFSFTPRVRATYFPDASDLDTVDYFGTLNWEHR
                     GQRTQTRVRGEFAQQDIVNSEQPDAEGGGDLGEGDFGDSGIAFFDNRRMRASLRPSMS
                     FELSPRRELQFDAGYTDVNYEEEFPGAQVDYTTADISAGLRARLNERSTLTTRLRGAR
                     YDLDTELRDVTNAYGAELQWDTTTAAETRTYFRAGAQNVEVTGGDSEVAWVAGAGVSF
                     LIGRNELFSDLSRNVGPSSAGVVVTRDQLRVRWTRAMTPRLSLLAGLRGTHDEDVDPV
                     STFVPRSYATGDVGLEWHWQEEFSLRAALDYTWQEFRDAASDATSSGAMVSLLYQPQQ
                     RRRARND"
     gene            8057..>8962
                     /locus_tag="VEW08_03875"
     CDS             8057..>8962
                     /locus_tag="VEW08_03875"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HYJ39903.1"
                     /translation="MDADNNPEFSGYIHAIARRKALLFGVAIPIAVLAVLLSVALPDV
                     YTSSALVEIDEPSSAASLSEGSSGESYADQYVQNLKGIVLTDSNLRKMIKEHDLYPGY
                     DDEVAALQRLKRDISVSIVTTPILDPRTGREREVVDAFTLSFDHREPAKAQKGAEWLV
                     SAFLAEHRRQRQGRATNAAQFYAKEAERVRTQVAGLESKLADFKRANAGQLPELTEVN
                     MSIMDRTESQLEQVTQQMSSLRQERVFLAAQLEQTRAAGPDAGSVRQLEDQYSRMRSS
                     YDESHPDMVQLRRQIDSLKFGTSAGT"
BASE COUNT         1656 a         2842 c         2821 g         1643 t
ORIGIN      
        1 gtgtattggc tgctgttcct gatttttcgc ttcctgctgc gccgtgacgt tggcgcggtc
       61 ggcatcgccg atatcctgct tctggtgatc gtcgcggacg ccgcgcagaa cgccatgtcg
      121 ggcggctata gcacctttgc cgaaggcgcg attctggtgc tcaccatcgc ctgctggaat
      181 tgggcgctcg atgtgctttc gtatcgattc agcgccgtcc gccgttttac gactcccgcc
      241 cgcctggcgc tgatcaagca gggcgtgctg cagcgtcgca atctgcggcg cgagtacatc
      301 accgtggaag agctgcacga gaagctgcgc gagcagggaa tcgagaaggt cgccgaggtg
      361 aaggccgcat acctcgaggg cgacgggcag atcagcgtga ttcgcaactc gggatcgcag
      421 aattcacagg ctccggagag ggcccttcca ggagcgtgaa cgccggtttt gcggcatcgg
      481 tggcgacgga caactccccc gtgtccggat gccactcgaa gacggcgcgt ccattggatt
      541 gcaggtcagc atggattccg gcacaaacac tgccgttcgg accgggcagg gaacgcgttt
      601 acgaaggtgc cgcatgcgcg gacggggcga tttacgctac cttgcgctcg cccgatcaat
      661 cggagcgtca cggagcggat gatgggtcac gaagaaaagg tcgttcagtg gttcaacgcg
      721 gtggattgga tccaggcggg ctcgcgtatt gccgccatcc tggtcgtcac tttcctgctg
      781 gtgttcgtcg cgcggcggtt gatctccggg ctcgagaaat tcaccgccac gcacgcacac
      841 gatgtcgaag cgcaaaagcg cgccgccacc gtgagcaacg tactgcgcaa gctggcaacc
      901 atcctgatca cggtgatcgc gctgctggcc gttttcaaca cgctgggcat ctccatcctg
      961 ccgttcctgg ctacggcggg agttgccggt atcgcgatcg cgtttggcgc gcagtcgctg
     1021 gtaaaggatt tctttcgcgg cttcttcctg ctggcggaaa accagattcg ccagggagac
     1081 gtggtcgaag tggcggggaa gagcggactg gtcgaagacc tgacgctgcg ctacgtgcag
     1141 ctgcgcgact acgagggcaa cgtgcactac gtgccgaatg gcgagatcgg cgtggtcacg
     1201 agctcgagcc gcacgtttgc gtatgcagtg gtggacgtca ccatcgcact cgaccaggac
     1261 attgcgcccg tcatcgactt gatgcgcgaa acgggaatgc agctccgcgc caattcggaa
     1321 ttcggcccca ggattctcga agacctcgac atcgccggca tcgaagcctg gaacgatcgc
     1381 ggcatcacgc tgcgcgcgcg cttcaaggtg cgggcgctgg agcagtcgaa cgtcaagcgc
     1441 gagtacctgc tgcggctcaa acatgcattc gacaaggcgg atattcagca gccgcgttcc
     1501 accgtcactt tcgtcgatcg cggtggcccg gcgacgcggg cgccggtgcc cgagggtcca
     1561 cccgcctgaa atagaaaagg ggtcaggcac cgtttgctag caaacggtgc ctgaccccat
     1621 tttttcatca atgggcggtg acggcgctct gggtggcggg tcccgagcgg cttgcgccgc
     1681 cttcgctttc ccagcgttcc ttttccaggc gctcatttag cagccgccgc tgttcctcgt
     1741 cgcggcgctg aaggcgcgcg gcgaagaatg ccgccgccag gatggcgatg cctgcgatac
     1801 ccagtacttt gatgatcttc atggtcagca tgctgttctc cgcgctggtc atttctgcaa
     1861 tctcttcagt tcggcgaggt gccgttcgat cgtggggagg gtctcgcgcg cgaacttgcg
     1921 cagatcctga tcctcgcccg ccttggcctg ggcgcgatac atcgccaccg agcgttcatg
     1981 ccccgcgatc atttcggcgg tccacttcga gtcgaagtcg ccgctcgcgg tgcccgacgg
     2041 aggcgcaccc tgcgcctgcg gagccggaac cggccagtgt ttggccccgg caagctgcga
     2101 caggcgcgca ttcgcgccgg tgtggtcgtt cacgagcatc tcggcgatgc gtttcaattc
     2161 gggttgctgc aattgcggca gcgcttcctt cgcggagttc acttccttgt agccgctctc
     2221 ggtggctttc ctcacgaagg tcatgtcgtc gatcggcttg cccacttcag tgccgcccgt
     2281 tgtcggggcg tcgaccgtgg tggtctggtt ggcctgcatg tccgccgcca tgagcggtac
     2341 gagcgaggcg cttccgggcg ccagggtttc gccaggcgcg agggctgcag aaggcaaagc
     2401 ctgttcgggc aacggctggg ccgggcccgc ggccggaaga ctgatcgccc acaccggcgc
     2461 cgccagaatg ctgatgactg caatcaaagg tagcggggcg cgtttcataa gtcctccaga
     2521 tgcaaagtcg gccgtccggc ggagtagtta gactccgccg gacggcccgt cacgcaacca
     2581 cggctatcag gtctcgcagg tgggcatcac cctggcgcga ttgcgttgtg cccttcagtt
     2641 cctagttcgt cggagccggt tcagcagagc cagcctgctt tgccgcgtcg agcgtcgcct
     2701 gggcctgggc cttggccgac tcatacatgg cctccgcctg gtccttgcat gccttctgcg
     2761 catcgctgct cagcgtttcg cacttttcct tggcgacctt gcgctggcca tcggcctgcg
     2821 ctaccgccac gtcgtactcg gctgcggcac gatccgctga gccttcctgg gcgacgtccg
     2881 cggcttcacg acgctcttcc gccacattcg cctgtgcgtc ctggcgctct tccgcgacgt
     2941 ccgttgccgt ttcgttggcc gactcacgtt ggttgcagcc ggcgagcgca cacatgccca
     3001 ccgctgcggc cactgccaat gtccagctgc cccgtccttg cttatcaagt gtcttggtca
     3061 cttttgaatc tcctgagtcc caaagggttc gaatggcaat cattcgtcgg ggggcatcct
     3121 gcaccgatga tctggagcga acacgcggac ttgaagtgga tactccgtca gacgtcaccg
     3181 accgcgtggc gattactcct gcattcgacg ccgaaagcag acaggtgact cctgtcagcg
     3241 atcacccaca cgggtcggtg tgtcgctggc ccaagatggc gtgcatctcc tgacagtgat
     3301 gcgctgcgcc gggccctaag gtggcggggc acatcaccct gaacttgaga attggaggag
     3361 atacatgacc gcccaggcaa agaatttcga gaatggcgcc cgaaacgccg aatccaccgt
     3421 catcgaactc aatcgcatcg tcgcgcaggc cgaagagctg ctgaggacac ttggcgaaga
     3481 gggcggcgca gccgttgaag ccgtacgcca gcgcgtgctg cgcaccgtga gccaggccaa
     3541 ggtaaaactg gccgatgtga gcacccgcgc ccgtggcgcc gcgaatgaag ccgccgaggt
     3601 cactgaccgt tacgtgcaca ccaacccgtg gaagtcgatc gcctatggag cggcggctgg
     3661 cgcggccgtt gcgctcattg ccgcggcact ggtgcgtcgc gactagtagc tagggaacgc
     3721 ggcgcccggc ccggccggtg cgccgccgac cgcaagcctc gctgacgagg gaaaatgcat
     3781 gtacgcaact gttgcactgg tcctgctgtt tgccaccgac ccacaacagg aactgaaaca
     3841 cgacgtgccg cagcccaggc aacaaggcac gcccacggat ccgctgttcg acaaggccta
     3901 cgtggccacc gacgaaccgg cgttcgtgct cagcgtcatc gaaagcaccc ggcaaggtga
     3961 agtcgatgcg cgtcttgcag cggaggaatt gggcaagccc gaacttcgcg acgctgcgaa
     4021 gcgtatcggg cgcctgaacg gctcgacgcg cgcgcggctc gaggtgctgg cgaaatccaa
     4081 aggctggcgc ctgcccgaaa agaatcccga tcgtgcgtcc acgctgccgg cgggtggcga
     4141 ttcacgcacg ggcgccaatt tcatcgtgca gcagatttcg tttcatgaaa gcaccgttgc
     4201 ccagttccgc gcgcagctga atggcagcgg cgaccaggat ctcaagcgtg agttgcgcga
     4261 ggcgctgccc ggctatgagc agaacctgga attgctgttg cgattgaagc tctgaattgt
     4321 ctgtcggaca gagcctcgtc ccgtgagccg cccggcccaa cggatgacgg cgtaatccag
     4381 cttcgcatgg cgcgaagtcc gacggcgcga tggctggcga tactggaatc ttcgctccat
     4441 gcacagaact gatcttgctc catttcacgc gcgctttccg cagggggcag cgcacacgtg
     4501 gctcacgcgc cgctggtact acaccccgcg ctcacaccgg gcatacagcg tgcggcagat
     4561 ttcccccggc gatcgtcgcc tgctggcgga gtttgcaatg accctcagtc agaactcgac
     4621 cgatcgcgag ctgccctgcg tgcaggattt gagcgacatg atgttcgagc gcgtgatcgc
     4681 cggcggcgtc gacaacgccg tgggctttgc agcactcgag aacaccgcgg gcggagaccg
     4741 ggtgatcggt gtcgctgcct atgcgccggc gagcgatgag agcgccagtt tcagtctcgc
     4801 ggtcgcgcat acgcatcgcg aagaacaggt ggggcgcatc ctgctttcca cgctggtacg
     4861 acatgcaaag cgcgtttgcg tcccgcgtct gaccggggag atgcactggt ccaaccggcc
     4921 gatgcagatg cttgccttgt cgatgggatt ctcggtcgag cccgagccgc gcgatcgcaa
     4981 tcttcgccgt ctcgtgctgc cgctcaaatg agccactaat caactacggg aggtcgagtg
     5041 tccgaatcct caagcggcgc cacattgcaa accggtacgc gcgagatcga gcaagtccgc
     5101 aaaggcgggc gggtgctcat catcgtcgag aacctgccgt gtcccttcga ccggcgcgtt
     5161 tggcaggaag cgcgaacgct ggccgcggcc ggctacgtgg tatcgatcat ctgcccgaaa
     5221 gcgccaggct atgaaaaaag cttcgagcag atcgatgaca tcgatatcca tcgccatgcg
     5281 cttcccgccg aggcggacgg ggtcctgggt tatgcgctcg agtacagcgt cgcgctcgcc
     5341 atggaattct ggctgtccct gaagattctc ttcggccgcg gcttcgacgt gatccacgcc
     5401 tgcaatccgc cggacacgat ctttctcatc ggggccttct acaagctgtt cggcaagaaa
     5461 tacgtattcg accaccatga catcaatccg gagctctacg aagcgaagtt cggcaagcgc
     5521 ggcttcggcc gccggctgct cgtggcgctc gagcgcatga gcttcaacac ggcggacatg
     5581 gtgatttcca ccaacgagtc gtatcgctcc attgccatcg aacgcggcgg gaagaatcca
     5641 aaggacgtgt acgtagtccg gtcggggccg gatctgtcgc gcatgaaagt ggtgccggcc
     5701 aatgccgcgc tcaagcacgg ccgcaagtat ctggtgggtt atgtcggcgt catgggtaaa
     5761 caggaaggaa tcgacctcct gcttcaggcg gtgcagctca tcgtaaagca gcttctacgc
     5821 accgacattc acttcggcct cgtcggcggt ggcacggagc tgcccgcgat gcgcgagctg
     5881 gccaaacagt taggcgtgtc cgagtacgtc actttcaccg gccgcgcgcc cgacgccgag
     5941 cttctcgaaa tgctgaacac ggcagacatc tgcgtgaatc cggaccgcgc caacgacatg
     6001 aatgatcgct cgacgatgaa caagatcatg gagtacatgg cgctcggcaa gccggtggtg
     6061 cagttcgacc tgaccgaagg acgcgtgtcg gcgggcgagg cgtcctggta cgcgcgcccg
     6121 aacgacgtcg ccgatctcgc cagcaaaatg gtcgcgctgc tcggcgacga aaaccagcgc
     6181 attcacatgg gcgccatcgg ccgggagcgc gtcgaacgcg agctttcctg gcaacacgaa
     6241 gcgccgcgcc tgctcgcggc atacgaacaa ttgactggag atcgatcatg aagaagtttc
     6301 tcttggcctg ctgcatcatc atggcgctgg cctgggcgac cggcgcgatg gcgcaggacg
     6361 ctaactacac ggtcaagcct ggcgacctgc tgtccatcag cgtatggaaa gagcccgacc
     6421 tgcagcggcc ggcactggtg cggcccgacg gctcgttctc ctttccgctg gttggcgagg
     6481 ttgacgccaa gggcaagacc gttgccgatc tcaacaaggc cgtgagcctg aagcttgcca
     6541 agtacatctc cgacccggtc gtcaccgtgt cgatacagga gatcaagggc aacaaggtgt
     6601 acgtcatcgg ccaggtgaac aagcccggtg agttcatcat gaatcccacc gtcgacgtga
     6661 tgcaggcgct gtcaatggcc ggcggtacca cgccgttcgc ggcgctcggc gacatcactg
     6721 tcctgcgtcg caccccgacg ggcaagcagt cacttccatt ccgctacaac gacatcatcc
     6781 gcggcaagag gctcgatcag aacatcgatc ttcagagcgg cgacgtggtg gtcgtaccct
     6841 aagtgaggta cgcaatgaaa cactcaacat tggtgatggc ggtggccgcc actctggcca
     6901 gcgcccaggc aggtgcggcc ccgtgggaat ggaatcccaa ggtcgaagcc ggatatctgt
     6961 acgacgacaa ctaccgcctg gcaactcccg gaagtgagat cgacgtgcag ggcccgatgg
     7021 tcgacgccga actcgaattg cgcacgctca cgcaacaggg tgaattcagt ttcacgccgc
     7081 gcgtgcgcgc cacctatttt cccgacgcct ccgatctcga caccgtcgac tacttcggca
     7141 ccctcaactg ggagcaccgg ggccagcgca cgcaaacccg cgtgcgcggc gagttcgcgc
     7201 aacaggacat cgtcaacagc gagcagcccg acgccgaagg cggcggggac ctcggtgagg
     7261 gcgatttcgg cgatagcgga atcgccttct ttgacaatcg ccgcatgcgt gccagcctgc
     7321 gtccgtcgat gagcttcgaa ctctcgccgc gccgggaact gcagttcgac gctggttaca
     7381 ccgacgtcaa ctacgaggaa gaattccccg gcgcgcaggt ggactacacc acggccgata
     7441 tttccgccgg ccttcgcgca cggctaaacg aacggtccac gctgacgacg cgccttcgcg
     7501 gcgcgcgcta cgacctagac accgagttgc gcgatgtgac gaatgcctac ggcgccgagc
     7561 tgcagtggga taccaccacg gctgccgaga cgcgcaccta ttttcgtgcc ggcgcgcaga
     7621 acgtcgaagt caccggtgga gattcggaag tcgcctgggt cgccggtgcg ggtgtgagtt
     7681 tcctcatcgg ccgcaacgaa ttgttttccg acctgtctcg caacgtcggt ccgtcgtcag
     7741 ccggtgtggt ggtgacgcgc gaccagctgc gcgtccgctg gacacgcgcc atgacgccgc
     7801 gcctgagcct gctcgccggc ttgcgtggca cgcatgacga agacgtcgac cccgtgtcca
     7861 cgttcgtgcc gcgctcgtac gccactggcg atgtcgggct ggaatggcac tggcaggaag
     7921 aattctcgct gcgcgccgcc cttgactaca cgtggcagga attccgcgat gccgcctccg
     7981 acgccacatc gagcggcgcc atggtttcgc ttctctatca accccagcaa cgtcgccgcg
     8041 caaggaacga ctgacaatgg acgccgataa caatccggaa ttttctggct acatccacgc
     8101 gatcgcgcgc cgcaaggcgc tgctgttcgg cgtcgccatt cccatcgcgg tgctcgccgt
     8161 gctgctctcg gttgcgctgc ccgacgtcta cacgtcatcg gcactcgtgg aaatcgacga
     8221 accatcgagc gcggcatcgc tgtccgaggg ttcgagcggc gagagttatg ccgaccagta
     8281 cgtgcagaac ctcaagggca tcgtgctgac cgacagcaac ctgcggaaaa tgatcaagga
     8341 acatgatctg tatcccggct acgacgacga agtcgcggcg ctccagcgcc tcaagcgcga
     8401 catcagcgtg agtatcgtga cgacgcccat tctcgatccg cgcaccgggc gcgagcgcga
     8461 ggtggtggat gccttcacgt tgtcgttcga ccatcgcgag ccggcgaaag cacagaaggg
     8521 cgccgaatgg ctggtcagcg cgttcctcgc cgagcaccgt cgccagcgcc aggggcgcgc
     8581 gacaaatgcg gcgcagtttt atgcgaagga agcagagcgc gtgcgtaccc aggttgcggg
     8641 tctcgagtcc aagctcgccg acttcaagcg tgctaacgcc ggacagcttc ccgagctcac
     8701 cgaagtgaac atgtcgatca tggatcgcac cgagagccag ctcgagcagg tcacgcagca
     8761 aatgagttca ctgcgccagg aacgcgtgtt cctcgctgcg cagctcgaac agacgcgggc
     8821 ggcgggaccg gatgccggca gcgttcgcca gctcgaagat caatacagcc gcatgcgctc
     8881 gagctacgac gagagccacc cggacatggt gcagttgcgc cggcagatcg acagcctgaa
     8941 gttcggcacc agcgcgggca cc
//
LOCUS       DASQJS010000097         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4449676, whole genome shotgun sequence.
ACCESSION   DASQJS010000097 DASQJS010000000
VERSION     DASQJS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4449676"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="VEW08_03880"
     CDS             complement(<1..1267)
                     /locus_tag="VEW08_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HYJ39904.1"
                     /translation="MSFISAAEADKNTYDVIVVGSGAAGGQTAYTLTMDGVRVLMLEA
                     GRNYTPATETPMMQTNAEAPLRGSGTPEKPFGFWDATIDGGWDVPGEPYTSASDDPAR
                     KFVWWRSRMLGGRTNHWGRISLRNGPYDFKPYSRDGLGFDWPMSYEDLAPYYDKVEML
                     IGVYGANDGLENTPNSPAGVLLPPPKPRVSDLLTKQRGAKLGIPVIAGHRAVLTKPLD
                     HTRLPKLLHPGNEKAQSILSADMQKRAACFFATPCGRGCSIRANYQSTTVHLPPALDT
                     GNLDIVTDAMVRTVTKRPDGSAEGVIYIDRKTGAEHRVKARIVVLAASALESVRILLN
                     SGVANSSGKVGKYIMDTVGANFSGQVPLLESLPLHNEDGVDGLHLYTPWWLYKEQLAG
                     KLGFARGYHYEFGGGRLMPGMGTAAGLEWL"
     gene            1384..2418
                     /locus_tag="VEW08_03885"
     CDS             1384..2418
                     /locus_tag="VEW08_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ39905.1"
                     /translation="MSAHLLGRRQLLQAAMATALPLSFYGCAGDKGAKREPLIVGGLP
                     VTCNLTLPIACTAKAASIKAGTASNDFEFEYSKYSGWPEIKESLMSNRIQAAYMLAPL
                     VMDLADKKIPVKIVSLGHRSGAVIMVRTDSAYQKFSDLKGKRVAIPSRFAVDFLFLRK
                     MLTRENMTPADIEIMEMPPPDMPAALYANAVDAYCTGEPFGAAAQRAGYARVLRMTRD
                     EWRNYICCCLTVRQELIDENPAMVQDLVNQVQGAGVWLDEKQENRDKAVAIAAGKGFF
                     NQDPNILKFVMENPTDRVTYGDLRMIREEFEDLMQLSIQAGTIKHPISYETYIDERFV
                     RAQKTASIPL"
     gene            2457..2993
                     /locus_tag="VEW08_03890"
     CDS             2457..2993
                     /locus_tag="VEW08_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYJ39906.1"
                     /translation="MALAAPEPAALKAGVLSPVMAAPGLKLQGSDGKPLDLARYRGKV
                     VLLAFGFSSCGEVCPITLATLAGARKKLGADAANVQVVYVTVDPERDDAARMKKYLAS
                     FDASFIGGVGSRAEIDAAQKSYGISSTKQVYADGNYTIGHSSSIYMIDRAGGLKAVMP
                     YGHTVADFVHDLKILLRT"
BASE COUNT          560 a          960 c         1020 g          572 t
ORIGIN      
        1 tgagccactc gagaccggcg gccgtgccca tgccgggcat cagccgtcca ccgccgaact
       61 cgtagtgata accgcgtgca aagcccaact tgcccgcgag ttgttccttg tataaccacc
      121 agggcgtgta caggtgcagg ccgtcgacgc cgtcctcgtt gtgcaacggc aggctttcga
      181 gcagcggcac ctggccgctg aagttcgcgc ccaccgtgtc catgatgtat ttgccgacct
      241 tgccgctgct gttggccacg ccggaattca gcaggatgcg aaccgattcg agcgcgctcg
      301 cggccagcac cacgatgcgc gccttcacgc ggtgctcggc gccggtcttg cggtcgatgt
      361 agatgacgcc ttccgcggag ccgtcagggc gcttggtcac ggtacgcacc atggcgtcag
      421 tgacgatatc gaggttgccg gtgtcgagcg ccggcggcag gtgcaccgtg gtggactggt
      481 agttagcccg gatcgagcag ccgcggccgc agggcgtggc gaagaaacag gcggcgcgtt
      541 tctgcatgtc cgcggagagg atcgactggg ccttctcatt gccgggatgc agtagtttgg
      601 gcagccgggt gtggtcgagt ggcttcgtta gaacggccct gtggccggca atgacgggaa
      661 tgccgagttt cgcgccgcgc tgcttggtga gcaggtcgct gacacgcggt ttcggcggcg
      721 gcagcaacac gcccgccggc gagttgggcg tgttctcgag gccatcgttg gcgccgtaaa
      781 cgccgatcag catttcaacc ttgtcgtagt acggcgcgag gtcttcgtag ctcatcggcc
      841 agtcgaagcc gagtccgtcg cgcgagtacg gcttgaaatc gtagggacca ttgcgcagtg
      901 agatgcggcc ccagtggttg gtgcgtccgc ccagcatgcg cgatcgccac cagacgaact
      961 ttcgcgccgg atcgtcggac gcgctggtgt agggctcgcc gggtacatcc caaccgccgt
     1021 cgatggtcgc gtcccagaag ccgaatggct tttcgggcgt tccgctgccg cgaagcgggg
     1081 cttcggcatt ggtctgcatc atcggggttt cagtggccgg cgtgtaattc cggcccgcct
     1141 cgagcatgag cacgcggacc ccatccatgg tcagggtgta ggcggtctgc ccgcccgcgg
     1201 cccccgaccc gacgacgatc acgtcatagg tgttcttgtc ggcttcggcg gctgaaatga
     1261 atgacatatt gggcgaatcc tatacggtgc cttgcgcgct gatagtcgaa aactatgcgg
     1321 tctcgggcta cgctgcgccg cactctggta ccagaccggc tcagccggca agtgaattcc
     1381 cacgtgagcg cgcatctcct cggaagacgt caactgctgc aggccgcgat ggccacggct
     1441 ttgcccttga gcttctatgg ctgcgcgggc gacaagggcg ccaagcgcga gcctctgatc
     1501 gtgggtggac tgccagtcac ctgcaatctc acgctgccca tcgcctgcac ggcgaaggcc
     1561 gcgagcatca aagcgggcac ggcgagcaac gatttcgagt tcgagtacag caagtacagc
     1621 ggctggcccg agatcaaaga gtcgctgatg tcgaaccgaa tccaggcggc ctacatgctc
     1681 gcaccgctgg tcatggatct tgccgacaag aaaattcccg tgaagatcgt ttcgctgggt
     1741 catcggtcgg gcgcggtgat catggtgcgc acggattcgg cgtatcagaa attctccgat
     1801 ctcaagggca agcgcgtcgc catcccgagc cgcttcgccg tggatttcct gttcctgcgc
     1861 aagatgctca cgcgcgagaa catgacgccg gcggacatcg agatcatgga gatgccgccg
     1921 cccgacatgc cggcggcgtt gtacgccaac gcggtggacg cgtattgcac gggtgagccg
     1981 tttggcgccg ccgcgcaacg cgccggttac gcgcgcgtgc tccgcatgac ccgcgacgag
     2041 tggcgcaatt acatctgctg ctgcctcacc gtgcggcagg aattgatcga cgagaatccg
     2101 gcgatggtcc aggacctcgt gaaccaggtg cagggcgcgg gcgtgtggct cgacgaaaag
     2161 caggagaacc gcgacaaggc cgttgccatc gccgctggca aagggttctt caaccaggac
     2221 ccgaacatcc tgaaattcgt gatggaaaac ccgaccgatc gcgtgactta cggcgacctg
     2281 cgcatgatcc gcgaggagtt cgaagatctc atgcagctgt ccatccaggc cggaaccatc
     2341 aagcatccga tctcgtacga aacctacatc gacgaacgtt tcgtacgcgc gcagaaaacg
     2401 gcctccatac cgctgtgatc cgcaggctcg cgctactgtt gatgttgagc tcaggcatgg
     2461 cactcgccgc gccagaaccg gctgcgctca aggccggtgt gctgagcccg gtcatggcag
     2521 ctcccgggct gaagctgcag ggttccgacg gaaagccgct cgatctggcg cgctaccgcg
     2581 gcaaagtggt gttgctggcg ttcggattca gctcctgtgg tgaggtctgt ccgatcacgc
     2641 ttgccacgct cgccggtgcg cgcaagaagt taggtgcgga cgcggcgaac gtgcaggtgg
     2701 tctacgtcac cgtggatccg gagcgcgacg atgcggcccg catgaagaag tacctggcga
     2761 gtttcgatgc gagcttcatc ggcggcgtgg gctcgcgcgc ggaaatcgac gccgcgcaga
     2821 aaagctacgg catcagctcc acgaagcagg tgtacgccga cggcaactac accatcggcc
     2881 actcgtcctc tatctacatg atcgatcgcg ccggaggcct gaaagccgtc atgccttatg
     2941 ggcacaccgt cgccgacttc gtgcatgacc tcaagatcct gctgcgcacg tgagcgggaa
     3001 aaagagactc tggatcgcgg tgccgctggt ggcggtcacc gcggcgctcg gatgggccgg
     3061 cttcgcgccc gtgagcgtgc catcgcgcga cgagttgttc gaaattccgc ag
//
LOCUS       DASQJS010000098         7698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3198154, whole genome shotgun sequence.
ACCESSION   DASQJS010000098 DASQJS010000000
VERSION     DASQJS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3198154"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1968)
                     /locus_tag="VEW08_03895"
     CDS             complement(<1..1968)
                     /locus_tag="VEW08_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ39907.1"
                     /translation="MTETNGAEAKYRQLFDTLIEGFCTIEMIFDAQGIPVDYRFLEIN
                     PAFERQTGLINAQGKRMRELAPNHEQHWFDIYGKIALTGESLRFENEAKALGRFYEVC
                     AYRVGGDESRKVAILFNDITERKAAETRIRAQNERMRLLHQITRAVGERQDLPSIYQV
                     VIRTLEDQMPVDFACVCLYDKAANDVMIASVGLRSAELAMELALTEQQHVNIGDDCVA
                     RAVLGEMIHEPDTSLVDSPFPKRLAKGGLRAMVAVPMLIEGVVFGVLVTARHAPNSFS
                     SGECEFLKHLSEHVAAAAQHAQLYAQLQVAYQDLRNTQAVAMQQERLRALGQMASGIA
                     HDINNAISPVSLYTESLLAREKGLSEAGRGQLQVIQRAIGDVAQTVARMREFYRPRAP
                     QLVLERVKLNELVQQVVDLTRVRWSDMPQQRGVTIALKLELANPLPNVMGADSEIREA
                     LTNIVFNAVDAMPDGGTMTLRTRPEEPARVVVEVTDTGNGMDEDSRRRCLEPFFTTKG
                     ERGTGLGLAMVYGMVQRHSGDIAIESELGKGTTVRLTFAAAVAEAPVQEVTHDEVPTG
                     LRLLLVDDDPVLLKSLRDALEADGHTIVTANGGRDGIDTFREAYESRQFFSAVVTDLG
                     MPYVDGRQVANAVKFIAPDAPVILLTGWGQRL"
     gene            2115..3911
                     /gene="kaiC"
                     /locus_tag="VEW08_03900"
     CDS             2115..3911
                     /gene="kaiC"
                     /locus_tag="VEW08_03900"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HYJ39908.1"
                     /translation="MPKTAPRPQFLKSLTGIRGFDEISLGGLPRNRTTLVMGGPGTGK
                     SVFALQSLVGAAGKYKQPGILVAFEENIEAIFANAAAFDWKLPALVKKKRLYFMNAQL
                     SPAFVSSGDFDLGGMLAMLEAKQMEIGAKWVVFDGIDVLLSLLRTPRAKMREIYRLRD
                     WLTRRELTSIITSKIEAAGDGTDTSNYGFMQFMVDCVVRFDRRMEQGIPVRRLEISKY
                     RGSNFAPGEYPLSFGPHGMEVASPGPTDVPQHASTERVSTGFPGLDEMLGGGLFRGSS
                     TLITGAPGTSKTTLAGLFAESACKRGERTLFVSFDEGGERVRRNLRSVGIHLDSHMKS
                     GLLQMYSGRTDAVNPEEHLIRIAALIAEHKPRCMVVDPLSAIARTGALSSARAIGNRL
                     IYKLRDHYITAVITALVEGQNPSAEATELQISTIADSWIQLSYLVCGGERNRALTIVK
                     SRGTGHSNQVRELVLSDNGLALSEVYSSGGEVLMGTLRWEKEAEDKSRALVSEAEFGQ
                     KRRELQFAEADTTARITALQLDLERQRAALALNTRTDVARDLTSGAHQTEVRRLRGGN
                     GVREAAEPGKRRSANVAKSRGNGNGRERVRGS"
     gene            3901..4221
                     /locus_tag="VEW08_03905"
     CDS             3901..4221
                     /locus_tag="VEW08_03905"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HYJ39909.1"
                     /translation="MAPKRSKQAAKSPVTTVVVMRLYVAGRAPNSVKAIANLEAICRE
                     HLKDAYKLEIVDVCEHPRRALDDGVLVTPSLAKLSPKPSANVIGNLSDTSSVLAALGL
                     TSVP"
     gene            4221..5528
                     /locus_tag="VEW08_03910"
     CDS             4221..5528
                     /locus_tag="VEW08_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYJ39910.1"
                     /translation="MLDAPDKSQAAFAQWVRRLVTGAAELRAFDAGEIDAVMDRDSGS
                     AVLLPEAQSALHDSNRIALSALDALPGEVCVLDANGVVVMTNKAWRVAGAAHERSGLD
                     VRAGENIFVACGDVPESERECAAAVSDAVRQVLTRMRRSLSYGYVCHAARTRPLCEFT
                     IAGVAGDGPVTAIVTREWDGEGPRAKKTPRPATRRSSKSSSLLQTAEKNHLLASLPAA
                     DYARMESGLEPVELTFGKVLYEPGEPMDAVYFPGNSLVSLLTLVEGHRALEVGLVGRE
                     GMIGARLALGAATSTVRALVLGTGTAMRMSTARFLKEFHRSPALQRALFQFTDLLMIQ
                     ISQTAACNRFHIVESRLARWLLMTSERMASPEFHLTHELLADMLGVRREGVTVAACAL
                     QQRKLISYRRGDITIVDTPGLKAASCSCYRYMQVQAAARTNGR"
     gene            complement(5529..6251)
                     /locus_tag="VEW08_03915"
     CDS             complement(5529..6251)
                     /locus_tag="VEW08_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYJ39911.1"
                     /translation="MNGLTLGAALLLGFAASGHCLVMCGGISAALGMAAAKDSQGRTR
                     LSLIMAYQLGRVFSYALAGLMLAGILGGLVSLLDIDSVRRTLRALTALALLLGALVAF
                     GGVRDPGSGIGRRLWPKLAPLGRRLLPIRTLPRGFAFGMVWGWMPCGFVYSVLLIATL
                     QLDAVQGALVMIAFGVGTVPALFIAACGAQRFSAFATRPVARHVGGSVLLLSAVLTLA
                     GPWIEHAMPGLHAWLPFDCQVR"
     gene            complement(6248..6409)
                     /gene="ccoS"
                     /locus_tag="VEW08_03920"
     CDS             complement(6248..6409)
                     /gene="ccoS"
                     /locus_tag="VEW08_03920"
                     /inference="COORDINATES: protein motif:HMM:NF015554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HYJ39912.1"
                     /translation="MNILLLLIPLSLLFVAGAVWVFFWAVNHGQFDDLETPGLLPLDD
                     SEKRRAPPS"
     gene            complement(6406..>7698)
                     /locus_tag="VEW08_03925"
     CDS             complement(6406..>7698)
                     /locus_tag="VEW08_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HYJ39913.1"
                     /translation="VLVRIERTGADTTLAGIAALVGRATAARPKLQIAGEEAAGRFVA
                     RVLALTALTATAWTVIDPARAFAAAIAVLVISCPCAFALAVPAALTRALGALARRGVL
                     VVKPDAIQSLAECTHAMFDKTGTLTEVSLSADVHTFGGVSRDDALSLAATVARESRHP
                     IARAIAAAHPGMQGASVSGVSTQAGLGVSATIDGRALRLGRADFALRKMGARENDEAV
                     LLADDSGPIAAFRVTERLRPDACAAIDALRARGMTVLIASGDAPSKVASIAARLNVAW
                     RARLLPADKLAWVAELRAGGALVLAVGDGVNDAPVLAGADLGVALAEGAELTQASGDI
                     VLKGARLDAIASARDIAQQTLAIVRQNQRWALYYNFSAVPLAALGFVPPWLAALGMSL
                     SSLGVILNALRIGRDETTRRPASQPQAPEETSPRAAAA"
BASE COUNT         1392 a         2550 c         2470 g         1286 t
ORIGIN      
        1 caagcgttgc ccccaacccg tgagcaggat gacgggtgcg tccggcgcga tgaatttcac
       61 cgcattggcc acctggcggc cgtcgacata gggcatgccg aggtccgtca ccacggccga
      121 gaagaactgc cggctctcgt acgcttcgcg gaaagtgtcg atgccgtcgc gcccgccgtt
      181 ggcggtcacg atggtgtggc cgtccgcttc cagcgcgtcg cgcagcgact tgagcagcac
      241 gggatcgtca tcgacgagaa gaagccgcag acccgtgggc acttcatcat gggtgacttc
      301 ctgcaccggc gcttccgcca ccgcggcagc gaacgtcaat cggaccgtcg ttcccttccc
      361 cagctcgctt tcgatggcga tgtcgccgct gtgccgttgc accatgccgt agaccatcgc
      421 caggccgaga ccggtgccgc gttcgccctt ggtggtgaag aacggctcga ggcagcggcg
      481 ccgtgaatcc tcgtccatgc cgttgccggt gtcggtaacc tcgacgacca cgcgtgcggg
      541 ttcttcgggt cgcgtgcgca gcgtcatcgt gccgccgtcg ggcatggcat ccaccgcgtt
      601 gaatacgata ttggtcagcg cctcgcgaat ttcactgtcc gctcccatga cgttcggcag
      661 cggatttgcg agttccagtt tcagcgcgat cgtgactcca cgctgctgcg gcatgtcgct
      721 ccagcgcacg cgcgtgagat ccacgacctg ctgcacgagc tcgttgagct tcacgcgctc
      781 gagcaccagc tgcggtgcgc gcgggcggta gaactcgcgc atgcgcgcca cggtctgcgc
      841 gacatccccg atggcgcgct ggatgacctg cagttggcct cggcccgcct cgctcaggcc
      901 cttctcgcgc gccagcagcg actcggtgta gagcgatacc ggtgaaatcg cgttgttgat
      961 gtcgtgcgca atgccgctcg ccatctgccc gagtgcgcgc aagcgctcct gttgcatcgc
     1021 gaccgcctgc gtattgcgca ggtcctggta ggcaacctgc aactgggcat agagctgcgc
     1081 gtgctgggcg gccgccgcca cgtgttcgct caggtgtttg aggaactcgc attcaccgct
     1141 gctgaagctg ttcggcgcat ggcgcgccgt gaccagcacg ccgaacacca cgccttcgat
     1201 gagcatgggc accgcgacca tcgcgcgcaa cccgcctttc gccagtcgct tggggaacgg
     1261 cgaatccacc aggctcgtgt ccggctcgtg gatcatctcg ccgagcacgg ccctggccac
     1321 gcagtcatcg ccgatgttca catgctgctg ttcggtgagc gccagctcca tcgcaagctc
     1381 ggcgctgcgc aggcccacgc tcgcgatcat cacgtcgttg gccgccttgt catagaggca
     1441 cacgcaggcg aaatccacgg gcatctggtc ttcgagcgtg cggatcacga cctggtagat
     1501 actgggcagg tcctggcgtt caccgacggc gcgcgtgatc tggtgcagca gacgcatgcg
     1561 ctcgttttgc gcacggatcc tggtttccgc agccttgcgt tcggtgatgt cgttgaacag
     1621 gatggcgacc ttgcggcttt catcaccgcc cacgcggtag gcgcaaacct cgtagaagcg
     1681 ccccagcgcc ttcgcctcgt tttcgaagcg cagcgactcg ccggtcaacg caatttttcc
     1741 gtagatgtcg aaccaatgct gttcgtggtt tggcgccagt tcgcgcatgc gctttccctg
     1801 cgcattgatc aacccggtct ggcgttcgaa ggcgggattg atctcgagga agcgatagtc
     1861 cacaggtatg ccctgcgcat cgaaaatcat ttcgatagtg cagaaaccct cgataagggt
     1921 gtcgaacagc tgccgatatt tcgcctctgc cccgttggtc tcggtcattc agatcaaatg
     1981 tagttgcctg attggtgaaa cgcaaaacca gacctgtagg aattcacctt ctatagtggg
     2041 ttctcgcagc aatttcacgt tgctatgagc gctaccgtac agaggagaca tgaatttgcg
     2101 cgcagcctgc ggcgatgcca aagaccgcgc cccgcccaca attcctgaaa tcgctgaccg
     2161 gcatccgggg attcgatgaa atcagtctcg gcggattgcc gcgcaatcgc acgacgctcg
     2221 tcatgggagg cccgggcacg gggaaaagcg tgttcgcgct gcagtcgctc gtgggcgccg
     2281 ccggcaagta caaacaaccc ggcatactcg tggcgttcga agaaaacatc gaggcgatct
     2341 tcgcgaatgc cgcagccttc gactggaaac tgccagcgct ggtgaaaaag aagcgcctgt
     2401 acttcatgaa cgcgcagctg tcccccgcat tcgtctcgag cggcgacttc gatcttggcg
     2461 gcatgctcgc catgctcgaa gcaaagcaga tggaaatcgg cgcgaaatgg gtggtattcg
     2521 atggcatcga cgtgttgctg tcgctgctac gcaccccgcg cgcgaagatg cgcgaaatct
     2581 atcggctgcg cgactggctg acacgccgcg agctgacctc catcatcacc tcgaagatcg
     2641 aagccgctgg cgatggcacg gatacctcca actacggatt catgcagttc atggtcgact
     2701 gcgtcgtgcg gttcgatcgc cgcatggaac agggcatccc cgtccgccgg ctcgagattt
     2761 cgaagtatcg tggctccaat ttcgcgcccg gcgaatatcc gctgagcttc ggtccgcatg
     2821 gcatggaagt ggcgtcgccc ggccccaccg acgtgccgca gcacgcctcg accgagcggg
     2881 tatcgaccgg ctttccgggg ctcgatgaga tgttgggtgg cggcctgttc cgcggcagca
     2941 gcactctgat caccggtgct cccggcacct cgaaaacgac actggccggg ttgttcgccg
     3001 agagcgcctg caaacgtggc gaacgtacgc tgttcgtgag ttttgacgag ggcggcgaac
     3061 gcgtacgccg caatctcagg tccgtcggca ttcatctcga ctcgcatatg aaatcgggtc
     3121 tgctgcagat gtattcaggg cgcaccgacg cggtgaaccc cgaagaacac ctgatccgca
     3181 tcgccgcgct gatcgccgag cacaaacccc gttgcatggt cgtcgacccg ctatcggcca
     3241 tcgcccggac gggtgcactg agttcggccc gcgccattgg caatcgcctc atctacaagc
     3301 tgcgtgatca ctacatcacc gcggtcatca ccgcgctggt cgagggccaa aacccgtcgg
     3361 cggaagccac cgagctccag atctccacga tcgccgacag ctggatccag ctctcctatc
     3421 tggtgtgcgg cggcgaacgc aaccgcgcgc tgaccatcgt caagtcgcgc ggcacggggc
     3481 attccaacca ggtacgcgag ctcgtgctga gcgacaacgg cctggcgctc agcgaagtct
     3541 attcctcggg cggcgaagtg ctcatgggca ctctgcgctg ggaaaaggaa gccgaagaca
     3601 agtcccgcgc gctggtttcc gaagcggaat tcggccagaa gcggcgcgag ctccagtttg
     3661 ccgaggcgga cacgacggcg cgcatcacgg ccctgcagct cgatctcgag aggcagcgcg
     3721 cggcgctggc gctcaacacc cgcaccgatg tcgcgcgcga cctgacttcc ggcgcacacc
     3781 agaccgaagt gcgcaggttg cgtggcggca atggcgtgcg ggaagcagcc gagccaggca
     3841 agcgacgctc cgcaaacgtg gcgaagtccc gcggcaacgg caacgggcgg gagcgcgtgc
     3901 gtggctccta aacgcagcaa acaagccgcg aagtccccgg tcaccacggt cgtggtcatg
     3961 cgcctgtacg tcgcgggccg tgcgccgaac tccgtcaagg cgattgccaa tctcgaagcg
     4021 atttgtcgtg agcatctgaa agatgcttac aagctggaaa tcgtcgacgt gtgtgagcat
     4081 ccgcggcggg cgctcgacga tggtgtgctc gtcacgccca gtctcgccaa gttgtcaccg
     4141 aagcccagcg cgaatgtcat cggcaacctg agcgacacca gcagcgtgct cgccgcattg
     4201 ggcctgactt cggtgccgtg atgctcgacg cgcctgacaa gtcgcaggct gcgttcgctc
     4261 agtgggttcg ccgcctggtg acgggcgcgg ccgagctcag ggccttcgat gcgggcgaaa
     4321 tcgacgcggt catggatcgc gacagcggca gcgccgtact gctgcccgag gcgcagagcg
     4381 ccctgcatga ctccaaccgc attgcgctga gtgcgctcga cgcactgccg ggtgaagtct
     4441 gcgtgctcga tgcgaacggc gtggtcgtca tgaccaacaa ggcctggcgc gtcgccggcg
     4501 cggcccatga gcgctcggga ctcgacgtgc gcgccggcga gaacatcttc gtggcctgcg
     4561 gcgacgtacc ggagagcgaa cgcgaatgcg cagcagcagt aagcgacgcg gtgcgccagg
     4621 tgctcacacg catgcgccgg tccttgagct acgggtatgt ctgccatgcc gcacgcactc
     4681 gtcccctgtg cgagttcacc attgccggtg tcgccggaga tgggccggtg accgcaatcg
     4741 tgacgcgcga gtgggacggc gaaggcccgc gggcgaagaa aacacctcgg ccggccacgc
     4801 gccgatcgag caagagttcc tcactgcttc agaccgcgga aaagaaccac ctgctcgcct
     4861 cgctgccagc cgcggactat gcgcgcatgg agagcggcct ggagcccgtc gagctgacct
     4921 tcggcaaggt cctgtacgaa ccgggcgaac cgatggacgc ggtgtatttt ccgggcaact
     4981 ccctcgtgtc gctgttgact ctcgttgagg gtcaccgtgc actcgaagtc ggactcgtcg
     5041 gccgtgaagg catgatcggc gcgcgcctgg cactgggcgc ggccacgtca acggtgcgcg
     5101 cactggtgct gggcactgga accgccatgc gcatgagcac ggctcgattt ctcaaggaat
     5161 tccaccgtag cccagcgctg cagcgagcgc tgttccagtt caccgacctg ctgatgatcc
     5221 agatcagcca gaccgcggcc tgcaatcgct ttcacatcgt ggaatcgcgg ctggcgcgct
     5281 ggctgctgat gacctccgaa cgcatggctt cgccggagtt tcacctgacg catgagctgc
     5341 ttgccgacat gctcggcgtg cgccgcgaag gcgtgaccgt ggcagcctgc gccctgcaac
     5401 agcgcaagct catcagctac cggcgcggcg acatcaccat agtcgacacc ccgggtctca
     5461 aggcggcgtc gtgctcgtgt tatcggtaca tgcaggtgca ggccgcggca cgcacgaacg
     5521 gtagatagtc agcgcacctg gcaatcgaaa ggcaaccagg cgtgcaggcc cggcatcgcg
     5581 tgctcgatcc aggggcccgc cagcgtcagt accgcgctga gcagcaacac cgatccgccc
     5641 acatgtcgcg ccaccggacg cgtggcgaat gccgagaatc gttgcgcccc gcaggcggcg
     5701 atgaagagcg ccggcacggt accaacgccg aacgcgatca tgacgagcgc gccttgcacg
     5761 gcgtcgagct gcagcgttgc gatcaacagc acggaataca cgaagccgca cggcatccag
     5821 ccccacacca tgccaaaggc gaaaccgcgt ggcagtgtcc tgatgggcag caggcgccgg
     5881 ccgagtggcg ccagctttgg ccagagcctg cgtccaatgc cggagcccgg atcgcgcacg
     5941 ccgccgaatg cgaccagcgc gccgagcagc aacgcgagtg ccgtcaacgc gcgcagggtg
     6001 cgccgcaccg agtcgatatc gaggaggctc acgagtccgc caagaatccc ggcgagcatc
     6061 aggccggcga gtgcgtagga aaaaacacga ccgagttgat aggccatgat gagagacagt
     6121 cgtgtgcgcc cctgcgaatc tttcgccgct gccattccga gcgctgcgga aatgccgccg
     6181 cacatgacga ggcagtgacc gctggcagca aagccgagca gcagtgcggc gccaagagtg
     6241 agcccgttca tgacggcgga gcccggcgct tttccgagtc gtcgagcggc agcagccccg
     6301 gagtctccag gtcatcgaac tgtccgtgat tgacggccca gaaaaatacc cacacggcgc
     6361 cagccacgaa cagcagcgac agcggaatca gcagcaggag aatgttcatg cggccgccgc
     6421 gcgcggagat gtttcttcgg gtgcttgcgg ctgactcgcc gggcgccgcg tcgtctcgtc
     6481 gcgcccgata cgcaatgcat tcaggatgac tcccagcgaa ctcagcgaca ttccgagcgc
     6541 cgccagccac ggcggaacga agccgagcgc cgcgagcggt accgcagaaa agttgtaata
     6601 gagcgcccag cgctggttct gacgcacgat ggcaagcgtt tgctgcgcga tgtcgcgcgc
     6661 gctggcgatc gcgtcgaggc gcgcgccctt gagcacgatg tcgccgctcg cctgggtgag
     6721 ctccgctcct tcggcaagcg ctacccccag gtctgcgccc gcgaggaccg gcgcgtcatt
     6781 gacgccatca ccgaccgcca gcacgagcgc gccgccagcg cggagctccg caacccacgc
     6841 cagcttgtcc gccggcagca ggcgcgctcg ccaggcgaca ttcaggcgcg cggcgatgct
     6901 cgcgaccttt gacggcgcat cgccgctcgc aatgagcacc gtcatccccc gcgcacgcag
     6961 ggcatcgatt gcggcgcagg catcgggccg caatcgctcg gtcacgcgga aggcggcgat
     7021 cggaccagag tcatcggcca gcagcacggc ttcatcgttc tcgcgcgcgc ccattttgcg
     7081 gagcgcgaaa tcggcgcgtc ccaggcgcag cgcgcgcccg tcgatcgtgg cgctcacacc
     7141 gagcccggcc tgtgtggaga cacctgacac gctcgccccc tgcattcccg gatgggcggc
     7201 ggcgatcgcg cgggctatcg ggtgacggct ctcgcgcgca accgtcgcgg ccaaactcaa
     7261 ggcgtcgtcg cgcgacactc cgccgaacgt atgcacgtcg gctgacagcg atacttcggt
     7321 gagcgtgccg gtcttgtcga acatcgcatg ggtgcattcg gccagcgact ggatggcatc
     7381 cggcttcacg accagcacac ctcggcgcgc cagcgcgccg agcgcgcgcg taagcgccgc
     7441 gggaacggcg agcgcgaacg cgcagggaca ggaaatcacc agcacggcga tggccgcggc
     7501 gaatgcgcgc gccggatcga tgacggtcca tgcggtggcg gtgagcgccg tcaatgccag
     7561 cacgcgggcg acgaatcgcc ccgcagcctc ttcgcccgcg atctgcagtt tcggacgtgc
     7621 ggcagttgcg cggccgacga gcgcggcgat tcccgcgagc gtcgtatccg cgccggtgcg
     7681 ctcgatgcgc actaacac
//
LOCUS       DASQJS010000099         6323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4449794, whole genome shotgun sequence.
ACCESSION   DASQJS010000099 DASQJS010000000
VERSION     DASQJS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4449794"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VEW08_03930"
     CDS             <1..290
                     /locus_tag="VEW08_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39914.1"
                     /translation="DPISMGVICRLIKQMNEALGITSIVVSHDVHELSTIADDSFLLS
                     GGRVVAQGTPAELHKSDLEEVRQFMGGLADGPVAFHYPARDLAQDLLEGEA"
     gene            287..1057
                     /gene="mlaE"
                     /locus_tag="VEW08_03935"
     CDS             287..1057
                     /gene="mlaE"
                     /locus_tag="VEW08_03935"
                     /inference="COORDINATES: protein motif:HMM:NF033619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HYJ39915.1"
                     /translation="MSAAVDSAPIAALRRIGSMSLFFIQLCARVIPAFAKPGLIVRQI
                     YNAGARSLVIIMLSGLFVGMVLGLLGFDTLQRFGSEDSLGVLAALGMLKVLGPVLTAL
                     LFAGRAGTSLTSEIGLMKSTDQLTAMQMMAVDPLRHVIAPRFLGGIIAMPLLAATFSV
                     IGLFGAQLIGVQQMGVDVGAFWSQTQNAVDLRDVNEGIVKSLVFGVACSLIAVYEGYN
                     SEPTPEGVGLATTRTVVTSSVMVLLLNYLLTAAFLTKS"
     gene            1069..1584
                     /gene="mlaD"
                     /locus_tag="VEW08_03940"
     CDS             1069..1584
                     /gene="mlaD"
                     /locus_tag="VEW08_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HYJ39916.1"
                     /translation="MKNSRSLEIGTGLFVLLGFAALAFLTTQLPGTSLKFSGNEGGYT
                     VTARFDNIGDLKPGSPVTMAGVRVGRVESIRFDPKDFKAAVVLRIEDQYKEIPEDSDA
                     SIQTAGLLGGKYIGIGPGGSETFLKQGGQIEFTQSAIVLESLVNKFFANMASKGPDEQ
                     KSDEPAAEEKK"
     gene            1581..2267
                     /locus_tag="VEW08_03945"
     CDS             1581..2267
                     /locus_tag="VEW08_03945"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ39917.1"
                     /translation="MMRSVKGLVQAAFMTGLLAAGAAQAAVDPLKPAPTAESRKVDTS
                     GPSQLIESSAQILLAGIDARRAEFHKDPTGLYELVANTLLPHFDTPYAAQLVLGQHWR
                     AATPEQRKRFVEAFYQSLLYTYGDAMVDFTGDRLKVLPTKVAPTDERATVRTEIKRSN
                     GTKVGVNYSLRKVGGEWKAWDVVIDGISYVRSYKEDYGAEVQQKGLESVIARLEAKAQ
                     SAKSAKVKST"
     gene            2298..2627
                     /locus_tag="VEW08_03950"
     CDS             2298..2627
                     /locus_tag="VEW08_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYJ39918.1"
                     /translation="MSEPARKNARVDISEVSDGRVTVSGDLTFASARDARQLGVLVLE
                     GSRAQRIMIDCNGVTRADSAGLAVLLDWLAWGRRNSRVVSLENLPASLVAIARISEVD
                     GLLTATG"
     gene            complement(2654..3787)
                     /locus_tag="VEW08_03955"
     CDS             complement(2654..3787)
                     /locus_tag="VEW08_03955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF008398.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HYJ39919.1"
                     /translation="MHVINSIEDLRAIARSRIPRALFDYIDRGSYDELTWKRNRDDVR
                     ALQLRQRVMVDVSRLSVATSVLGEPWKMPVALAPTGLTGFFHRDGEIEAARAAHAAGV
                     PYCLSTMSICSIEDVRAAVEGNFWFQLYLMRDRGFNEALITRAREARCSALMLTLDLP
                     LQALRRRDPKNGLSVPPRLTAANAWEILLRPRWLSSVLLGKRRTLGNLASFFPERGIG
                     ELSEWVGGQFDPSITWKDVAWVRERWPGKLIVKGILDAEDARLAHAAGVDAIVVSNHG
                     GRQLDGATSTIAALPRVVDAVAGGCEVLLDGGISSGQDVLKALALGARACLTGKAYLY
                     GLSALGGKGVTLALEIIRRELEISMALTGLDDVRNVNSRILVA"
     gene            complement(3819..4664)
                     /locus_tag="VEW08_03960"
     CDS             complement(3819..4664)
                     /locus_tag="VEW08_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYJ39920.1"
                     /translation="MKLVRFGSAGAERPGLVLADGSIADLSGVIPDLAGAALEPASLK
                     KLSAVDAAKLPKAPGGVRLGACVGGVGKFIAIGLNYADHAAETGAKIPAEPIVFMKAT
                     SCIVGPNDTVLKPRGSIKMDWEVELGVVIGSVTRYVSEADAPNHIAGYCVINDLSERH
                     FQLERGGQWDKGKGCDTFGPLGPWLVTSDEVGDPANLSMWLDVNGTRRQNGSTRTMIF
                     KPAFLVSYLSQFMSLQPGDVITTGTPPGVGMGIKPEPIFLNVGDVMELGIEKLGSQRQ
                     VVAAA"
     gene            complement(4681..5868)
                     /locus_tag="VEW08_03965"
     CDS             complement(4681..5868)
                     /locus_tag="VEW08_03965"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HYJ39921.1"
                     /translation="MQGFLRRDGRKGIRNVVAVAYLVECAHHVSRLIVQKSGSDDVHL
                     IGFPGCYPNKYSLEMMQRLCTHPNVGAVLLVSLGCEGFNREQLRRTIAASGRPVDLLV
                     IQQSGGTTRTLNAGLDIVRKMRAELARQTPVPMAFDELIVGTVCGGSDGTSGITANPA
                     VGRCFDWLIEKGSACIFEETGELIGCERIMASRAITPELGREIEACVDKAANYYRIMG
                     YGSFAPGNAEGGLTTLEEKSMGAYSKSGSSPISGLIKPGDIPPRGGLYLLDVVPDGEP
                     RFGFPNISDNAEIVELIACGSHLILFTTGRGSVVGSAISPVIKVCANPDTYDRMADDM
                     DVNAGRILRGAELDDVGREIFELVQSVARGALSKSEALGHQEFILTYKQFEPIGPACL
                     PAA"
     gene            complement(5895..6221)
                     /locus_tag="VEW08_03970"
     CDS             complement(5895..6221)
                     /locus_tag="VEW08_03970"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HYJ39922.1"
                     /translation="MTTLDKGKLPPLLLLHPDDNILVARRDIALGELVEMDGETFNMP
                     VAIELGHKLARRALAPDTRVLKYGAPIGSMKIAVARGEHVHLHNLRSDYLPSTSRDME
                     KKTDGH"
BASE COUNT         1148 a         1975 c         2063 g         1137 t
ORIGIN      
        1 tcgatcccat ttccatgggc gtgatctgcc ggctcatcaa gcagatgaat gaagcattgg
       61 gcatcaccag catcgttgtc tcacacgatg tccacgagtt atcgacgatc gccgacgaca
      121 gcttcctgct ctcgggcgga cgtgtcgtgg cgcagggcac acccgcggaa cttcacaaga
      181 gcgatctcga agaagtacgc cagttcatgg gcgggctcgc cgacgggccg gtggcgttcc
      241 actaccccgc gcgcgatctt gcgcaggact tgctggaggg cgaggcatga gtgctgcagt
      301 cgattcagcc ccgatcgccg cgttgcgccg catcggcagc atgtcgctgt tcttcatcca
      361 gctctgcgcg cgcgtcattc ccgcattcgc gaagccgggc ctcatcgtcc ggcagatcta
      421 caacgccggc gcgcggtcgt tggtcatcat catgctctcg ggcctgttcg tcggcatggt
      481 gctgggcctg ctcgggttcg acacgctgca gcgtttcggc tccgaagatt cactcggcgt
      541 gctcgcggcc ctcgggatgc tcaaggtgct ggggccggtc ctgaccgcgc tgttgtttgc
      601 cggccgcgca gggacctcac tcacgtccga gatcgggctc atgaagagca cggaccagtt
      661 gacggccatg cagatgatgg cggtcgatcc gctgcgccac gtcatcgcgc cgcgattcct
      721 cggcggcatc atcgccatgc cgctgctggc cgccacgttc agcgtcatcg gcctgttcgg
      781 tgcacagctc atcggcgtgc agcagatggg cgtagacgtg ggcgcgttct ggtcgcagac
      841 ccagaacgcg gtcgacctgc gcgatgtcaa tgaaggcatc gtcaagagcc tcgtgttcgg
      901 cgtggcctgc agcctgatcg ccgtatacga gggctacaac agcgagccga cacccgaagg
      961 cgtgggcctc gccacgacgc gcaccgtggt cacctcgtcg gtcatggtgc tgctgctcaa
     1021 ttatttgctg accgcagctt tcctaacaaa gtcctgatcg gaacgtttat gaaaaactcc
     1081 cgctccctcg aaatcggcac cggcctgttc gtcctgttgg gctttgccgc gctggcattc
     1141 ctgaccacgc aactgccggg aacgtcgctc aagttcagcg gcaacgaggg cggatacacc
     1201 gtcacggcca ggttcgacaa catcggcgac ctcaagccgg gttcgcccgt caccatggcg
     1261 ggcgttcgcg tcggccgcgt ggagtcaatc agattcgacc ccaaagactt caaggccgcg
     1321 gtcgtgttgc gcatcgagga tcagtacaag gaaatccccg aagacagtga cgccagcatc
     1381 cagacggccg gtttgctcgg cggcaaatac atcggtatcg ggcccggtgg atcggaaacc
     1441 ttcctcaaac agggcggtca aatcgaattc acacagtcgg ccatcgtgct cgagagtctg
     1501 gtcaacaagt tcttcgccaa catggcgagc aaaggtccag acgaacagaa gagcgacgag
     1561 ccggcagcag aggagaagaa atgatgcgat cagtaaaggg attggttcag gctgcgttca
     1621 tgactggttt gttagccgct ggcgcggcgc aggctgcggt cgatccgctc aagcccgccc
     1681 cgaccgccga gtccaggaag gtggatacca gcgggccatc ccagctcatc gaaagctcgg
     1741 cgcaaatcct gctggccggc atcgatgcgc gtcgcgcgga attccacaag gatcccaccg
     1801 ggctatatga gctcgtggcc aacacgttgc tgccgcattt cgacactccc tacgcggcgc
     1861 agctcgtgct cggtcagcac tggcgcgccg cgacgccgga gcagcgcaag cgcttcgtcg
     1921 aggcgttcta ccagtcgctg ctgtacacct acggcgacgc catggtggat ttcaccggcg
     1981 atcgtctcaa ggtgttgccg acgaaggtcg cgcccacgga cgaacgcgcc acggtacgca
     2041 ccgagatcaa gcgctccaat ggcaccaagg tcggcgtcaa ctacagcctg cgaaaggtgg
     2101 gtggcgaatg gaaggcgtgg gatgtggtca tcgacggcat cagctacgtg aggagttaca
     2161 aggaagacta cggcgccgaa gtccagcaga aggggctcga gtccgtcatt gcgcgcctcg
     2221 aagccaaggc gcagagcgcc aagtccgcga aggtgaaatc gacctgagag gggcaaccga
     2281 tccatgagcc ctgagaaatg agcgagccgg ccaggaaaaa cgcgcgcgtc gatatcagtg
     2341 aggtcagcga tggccgggtc accgtatcgg gcgatctcac gtttgccagc gcgcgggatg
     2401 cgcgccagct cggggttctc gtgctcgaag gatctcgcgc tcagcgaatc atgatcgact
     2461 gcaacggcgt cacgcgcgcc gatagtgcgg ggctcgctgt cctgctcgat tggctggcgt
     2521 ggggccggcg caactcacgc gtggtgtcac tcgaaaacct gccggcgtcg ctggtggcga
     2581 ttgcgcgaat ttccgaagtc gacgggctgc tgaccgcaac cggttaggcc gcatggctcg
     2641 agcctcgcgt ggttcaggcc acgagtatgc gcgaattgac gttgcgcacg tcgtcgagtc
     2701 cggtcaaggc catcgatatt tcgagttcgc gccggatgat ctccagggcg agcgtcacac
     2761 ccttcccgcc gagcgcggac aatccatata gataggcctt gccggtgagg caggcgcggg
     2821 cgcccagcgc cagcgctttc agcacgtcct ggcccgagct gatgccgcca tcgagcagca
     2881 cctcgcatcc accagcgacg gcatcgacca ctcgcggcaa cgccgcgata gtcgaagtcg
     2941 caccatcgag ctgacgtccg ccgtgattgg acacgacgat ggcgtcgacg ccggccgcat
     3001 gcgcgagtcg tgcatcttcg gcgtcgagga ttcccttgac gatgagtttg cccggccagc
     3061 gttcacgtac ccaggcaaca tctttccagg tgatcgacgg atcgaactgg ccaccgaccc
     3121 attcagagag ctcgccgatg ccgcgttccg ggaaaaagga cgcgagattg ccgagggtgc
     3181 gccgctttcc aagcagcacg ctgctgagcc agcgcggacg caagaggatt tcccatgcgt
     3241 tcgccgcggt cagccgcggc gggacggaca ggccgttttt tggatcgcgt cgccgcaggg
     3301 cctgcaaggg caggtcgagc gtcaacatga gcgcagaaca gcgcgcttcg cgcgcacgcg
     3361 tgatcaacgc ctcgttgaat ccgcgatccc gcatgagata gagctggaac cagaagttgc
     3421 cttccacggc cgcgcgcaca tcttcgatcg aacagatgga catcgtgctc aggcagtacg
     3481 ggacgccggc cgcatgcgcg gcgcgtgccg cctcgatttc gccgtcccta tggaagaatc
     3541 ccgtgagtcc ggtcggcgcc agcgcgacgg gcattttcca tggctcgccg agcaccgaag
     3601 tggcgaccga gagccgcgac acgtcgacca tgacgcgctg gcggagctgc agcgcgcgca
     3661 catcgtcgcg attgcgcttc caggtcagct cgtcgtagga accgcggtcg atgtagtcga
     3721 acagggcgcg tgggatgcga ctgcgcgcaa tggcgcgcag gtcttcgatg gagttgatca
     3781 cgtgcatgag cggccaactt ccgccggcgc gggccgtctt acgccgcggc cacgacctgg
     3841 cgctgactgc ccagcttctc gatgccgagc tccatcacat cgccgacgtt caggaaaatc
     3901 ggctcgggct tgatgcccat gccgacgccg ggtggtgtgc ccgtggtgat cacgtcgccc
     3961 ggttgcaggc tcatgaactg gctcaggtaa ctcacgagga acgcgggctt gaagatcatc
     4021 gtgcgcgtcg aaccgttctg ccggcgggtg ccgttgacgt cgagccacat cgaaagattg
     4081 gcgggatcgc cgacttcatc cgaagtaacg agccacggac caagcggtcc gaaagtgtca
     4141 cagcccttgc ccttgtccca ctggccgccg cgctcgagct ggaaatgccg ctcggagagg
     4201 tcgttgatca cgcagtaacc cgcgatgtga ttaggcgcgt ccgcctcgct tacatagcgc
     4261 gtgacgctcc caatgacgac gccgagctcg acttcccagt ccatcttgat ggagccgcgc
     4321 ggcttgagca ccgtgtcgtt ggggccgacg atgcagctgg tggctttcat gaacacgatg
     4381 ggttcggcgg gaatcttcgc gccggtttcc gcggcgtgat ccgcgtagtt gagtccgatg
     4441 gcgatgaact tgcccacgcc acctacgcag gcgcccaggc ggacgccgcc cggagcttta
     4501 ggtagtttgg cggcatcgac ggcggagagc ttcttcagcg acgcaggctc gagtgcggct
     4561 ccggcaagat cggggatgac gccggaaagg tcggcaatgc tgccgtcggc gagcacgaga
     4621 cccgggcgct ccgcgcccgc agagccaaaa cgtacgagtt tcaagagact ctcccggaat
     4681 ttacgcggcc ggcaggcagg ccggaccgat cggttcgaat tgtttgtagg tgaggatgaa
     4741 ttcctggtgg cccagcgcct cggacttcga caacgcgcca cgcgcgacgc tttgcaccag
     4801 ctcgaagatt tcgcggccca cgtcatccag ttcggcgccg cgcaggatgc gaccggcgtt
     4861 cacgtccatg tcgtcggcca tgcggtcgta agtgtccgga ttggcgcaga ccttgatgac
     4921 cggcgaaatg gcggagccca ccaccgaacc gcggccggtg gtgaacagga tgaggtgtga
     4981 accacaggcg atgagctcga cgatttcggc gttgtcggag atgttgggga atccgaaccg
     5041 cggctcgccg tcgggtacga cgtcgagcag gtagagcccg ccgcgcggcg gaatatcgcc
     5101 gggcttgatg agcccgctga tgggcgagga gccggacttg gagtacgcgc ccatggattt
     5161 ttcttcgagc gtggtcaggc cgccttcggc gttgcccggc gcgaagctgc cgtaacccat
     5221 gatgcggtaa tagttagcgg ccttgtcgac gcaggcttcg atttcacgac cgagctcggg
     5281 cgtgatggcg cgcgaggcca tgatgcgctc gcagccgatg agctcgccgg tttcctcgaa
     5341 aatgcaggcc gagcctttct cgatgagcca gtcgaagcag cggcccacgg cggggttggc
     5401 ggtgatgccg ctggtgccgt ccgaaccgcc gcacacggtg ccgacgatga gttcgtcgaa
     5461 cgccatgggt accggagtct gtctggcgag ctcggcgcgc atcttgcgca cgatgtcgag
     5521 gcccgcattg agcgtgcgcg tggtgccgcc ggattgctgg atgaccagca ggtccaccgg
     5581 ccggccgctc gcggcaatgg tccggcgcaa ctgttcgcga ttgaagccct cgcagccgag
     5641 cgacacgagc aacacggcgc cgacgttcgg atgcgtgcac agccgctgca tcatctccag
     5701 cgaatacttg ttggggtaac agccggggaa gccgatgagg tggacgtcgt cgctgcccga
     5761 cttctgcacg atgaggcgcg aaacgtggtg cgcgcattca accaggtacg caaccgcgac
     5821 cacattgcgg atgcccttgc ggccatcgcg tcgaaggaac ccttgcatcg aagggcttgt
     5881 cgccaccgcg ttcatcagtg tccgtccgtc ttcttttcca tatcccgtga ggtcgaaggc
     5941 aggtaatcgc tgcgcaggtt gtgcagatgc acgtgttcac cgcgcgccac cgcgatcttc
     6001 atggagccga tgggggcacc atacttgagg acgcgggtat cgggcgcaag ggcccggcgg
     6061 gcgagtttgt gaccgagctc gatcgccacc ggcatgttga aagtttcccc gtccatttcc
     6121 acgagctcgc ccagcgcgat gtcgcgccgc gcgaccagga tgttgtcgtc agggtgcagc
     6181 agcagcaacg gcggcaattt gcccttgtcc agtgtcgtca tggttcgccg ctgtccctta
     6241 agtctagcta ttgcgccggc ggaggcggtg ccggcacttc ggtgggcgga gtttccggag
     6301 ccggctccgc cggatcgtcc ttg
//
LOCUS       DASQJS010000100         9192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_54128, whole genome shotgun sequence.
ACCESSION   DASQJS010000100 DASQJS010000000
VERSION     DASQJS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54128"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VEW08_03975"
     CDS             complement(<1..788)
                     /locus_tag="VEW08_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39923.1"
                     /translation="MKHIDRRTLITGALAGLTTLRTQAASTASSARLTLRPDLVLNHL
                     PDSYNGFSIETATLENPDVYHPANTSLVSLFRELTPHGVLRLGGNSSEFCWWKANEQT
                     QRPEIHVEGHGRTDNWMPQKFHAITPRAIDNLRVFLDACGWTCIYGLNFGTGSPERNA
                     AEAAYVAPALGDRLRYFQIGNEPDFYRAENNLLRPRGWDFPDYLSEWMAIARAVISAV
                     PGARFGGPDVGSSADWVVRFGNEAPKLLEGRLLELTGHYYAEGPP"
     gene            827..2443
                     /locus_tag="VEW08_03980"
     CDS             827..2443
                     /locus_tag="VEW08_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HYJ39924.1"
                     /translation="MRILQIALVLGVAAASAGAARAAQPLPEIVTRDGRYALIVDGAP
                     YLVLGAQVNNSSNWPAMLPKVWPAVKKLQANTVSVPIGWEQVEPVEGRFDFSFLDTLL
                     AEARENHVRLVLLWFATWKNNGPNYAPEWVKLDNARFPRLVDARGKVLNSMSPHSAAM
                     LAADKRAFVALMKHLKSADEARTVIMVQVQNEPGTYGSARDFGPAAQKLFDGPVPAAI
                     IKAQGAKPGTWTQAFGKDANEYFQAWAVAKFIGEIAAAGKKEYPLPMYVNAALRDPFH
                     PGNAGDYASGGPTDNVISIYRAAAPAIDIVAPDIYMKESNKAYRVIELYKPGGPLLVP
                     EIGNDPLFARYFYDVLGAQAIGIVPFGIDYTGYSNFPLGAKDVDAGIDAFARPYRLLA
                     PMAREWARLSFTNKVWGASEPDDRSSRKLDLGRWQATLSFNEWQFGMKEWFGDLKDKP
                     EWSGAPSGGALLVQLGADEFLLTGQHVRVSFAPAAGRKANGLIFASVEEGSYVDGKWS
                     RTRVWNGDQTDYGLNLTDEPRVLRVRLATY"
     gene            complement(2440..3174)
                     /locus_tag="VEW08_03985"
     CDS             complement(2440..3174)
                     /locus_tag="VEW08_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004827669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ39925.1"
                     /translation="MESKRLAGKIAVVTAAGSGIGRASAEAFARAGARVIAADIDGNA
                     LKNVVGCEGQVLDVRDPVAIAAFAKATGKVDVLFNCAGIVPGGTVLTVTPEQWAAAFE
                     LNVHSMFSMIRAFLPAMLEARSGSIINMSSVASSITGVPDRCAYGATKAAVIGLTKSV
                     AADFVAQGIRCNAICPGTVDTPSLQARLHAYGDYARTRAEFEARQPIGRLGKPEEIAA
                     LALHLASDESGFTTGQIHVIDGGWMI"
     gene            complement(3164..3991)
                     /locus_tag="VEW08_03990"
     CDS             complement(3164..3991)
                     /locus_tag="VEW08_03990"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ39926.1"
                     /translation="MSTRVDSHQHFWRLERGDYGWLTPALTPIYRDCLPEHLAPHLAE
                     AGVGATILVQAAATVAETRFMLELARDHSFIAGVVGWVDFESEDVVETIATLAADPAL
                     VGLRPMIQDIPDTEWMLEEKLAPAFEAMIDHGLVFDALVLPKHLPALLELTARYPDLV
                     MVLDHGAKPPIAAGDIEPWKQAITELARSSPMTCKLSGLVTEAGSANAAALEASVEHL
                     LGAFGPARMMWGSDWPVCELVCSYGEWRDVTDTLLAKLKTTEREQIYSATARATYGI"
     gene            complement(4000..5022)
                     /locus_tag="VEW08_03995"
     CDS             complement(4000..5022)
                     /locus_tag="VEW08_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ39927.1"
                     /translation="MTLARRELGNSGLEVTELGFGGAAIGNLYRELPDDEASAAVRES
                     FAAGVRYFDTAPFYGFGLSEIRLGQALQGAQALPVISTKVGRRLVPTGPQDASAGREG
                     YFSPRPFAPVFDYSYESVMRSHAESLERLKVARIDILLCHDIGHMTHGTAHAARVAEF
                     LEGGYRAMRELRDAGTVRAIGLGVNEWEVCVELLADCELDCILLAGRYTLLEQPALET
                     LLPLCEQRRVSIICGGPFNSGILAAGSRAGAKTHYNYAAPPPAVLERVTRLEQLCGEF
                     SVPLQAAALQFPLGHPRIASVVAGCANGAEARNCAAMYSHPIPSGFWRALRERGLVDV
                     RAPLPV"
     gene            complement(5019..6317)
                     /gene="fucP"
                     /locus_tag="VEW08_04000"
     CDS             complement(5019..6317)
                     /gene="fucP"
                     /locus_tag="VEW08_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HYJ39928.1"
                     /translation="MNDAALPAKPKAAFITPGFLFATILIVGLFFMWGVANSLNDVLI
                     PQFRKAFQLDDFASSLVQSAFYFGYFCFAIPASLFMRRFGYRAAVLMGLGLYGAGALL
                     FLPAAQASQYYAFLIALYVIASGLAFLETSANPLIAVMGPAGTADQRLNFAQTFNPIG
                     TMMGSYVGGMLIFSDVHYSPEQLQALTPDAFAAFRATELDSVKLPYVLIAAMVILWAV
                     LVACAKFPPVDRGRGHDTGEAEGGFGGLGKFPRYWLGVLAQFAYVGAQVGVWSFLIRY
                     TQHNFPGTLEKDALRWLPISFGIFFAGRLIGTLLMSRINPAKLLASFAAINVALCLIA
                     VVSGGHVGLTALIASSFFMSIMFPTIFSMSLRGLGVYTKSGSSFLVMAIIGGAVFTAA
                     MGFISRESTINLAYIVPAVCFAVVFLFAWKSRRDTVTAIA"
     gene            complement(6314..7117)
                     /locus_tag="VEW08_04005"
     CDS             complement(6314..7117)
                     /locus_tag="VEW08_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ39929.1"
                     /translation="MATKTAPPRLPAQETAPRTSRYRAPALEKGLDVIELLAAEKSPL
                     NLSSISQRLGRSSGELFRMLQVLEFKGFITTAENGSGYVLTNKLFALAMAQAPVRSLV
                     ETALPIMRKLVQDIGQSAHIAVASEDQIVVITRIERPGDLGFSVRIGYRREIASATSG
                     LVLFAYQNDDVRRIWLKRCRLKGEAADSFVERANIVRSKGYHEAASHFVRGIVDLSAP
                     ILRDDTAVAALTIPFVHSNPLQVEMAHAVEFVRAAAKQISNEIVHGDLP"
     gene            7396..>9192
                     /locus_tag="VEW08_04010"
     CDS             7396..>9192
                     /locus_tag="VEW08_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HYJ39930.1"
                     /translation="MKMLTRWMCVLVLAGCAAEAPEGSFVQNETGIVVTPADGASRRV
                     RLEVKTDRIVRVTSVTDGNLDLPKSLMVVDSPAKPVAFQVERRDSDVVLKTSQIAAHV
                     SLANGAVSFSDLAGKPLLAEEPFRKLESGVTQRFNAGTDEAFYGSGQHQNAQMNLNGE
                     DVELAQHNMDIGVPFVVSTRNYGVLWDNNGITRLGDPKPYGLASRDLRIRDVAGKDGG
                     FTARYYIDGKQKIERVEKDINYQYIRDRFNWPKELLVAKEPNTGSPPNILANQTVTWE
                     GTLESAKAGKHKFQLYGSSYFKVYVDGRLVMDRWRQNWAAWYHNFDVVMTAGIPVAVR
                     IEWIPNDGYLALLHNDPLPDAERHSLTFTSDAASAVDYYFIAGASQDEVIAGYRALTG
                     KAVMLPRWAYGFWQSRQRYTTQKELLDVVAEYRKRKIPLDNIVMDWFYWKEDQWGSHQ
                     FDKSRFPDPKAMLEKVHGMNAHFMISVWPKFYPGTDNYKELDSAGFMYRGNIEAGALD
                     WVGKGYQNSFYDPYSKPARDMYWRQLNENLNVLGIDAWWMDATEPDLHSNLDIDSIKA
                     RIGPTAMGPAEKFFNSYPLVHTGGVYEGSRVVS"
BASE COUNT         1779 a         2812 c         2987 g         1614 t
ORIGIN      
        1 ggcggtcctt ccgcgtagta atggcccgtg agctccagca gccggccctc cagcaacttc
       61 ggcgcttcat tgccgaagcg caccacccag tccgccgacg aacccacgtc cggtccaccg
      121 aaccgcgcac cgggcaccgc gctgatgacc gcgcgcgcaa tcgccatcca ctcggataga
      181 tagtcaggga agtcccagcc gcgtggccgc agcaggttgt tctcggcacg gtagaagtcc
      241 ggctcattgc cgatctggaa gtagcgcagc cggtcaccca gcgcgggcgc gacgtacgcc
      301 gcttcggcgg cgttacgctc gggcgagccg gtgccgaagt tcagcccgta aatgcaagtc
      361 cagccgcagg catcgagaaa tacccgcaga ttgtcgattg cgcggggtgt gatggcgtga
      421 aatttctgcg gcatccagtt gtcggtacgg ccgtgccctt cgacatgaat ctccgggcgc
      481 tgggtctgct cattcgcttt ccaccagcag aactcgctgc tgttgccgcc gagccgcagt
      541 acgccgtgtg gagtcaattc ccggaacagg gataccagcg aggtgttggc aggatggtag
      601 acatcggggt tctcgagcgt cgcagtctcg atgctgaagc cgttgtagga gtccggcagg
      661 tgattgagca ccaggtccgg tcgcaaggtg agacgcgcgg aacttgccgt cgaagccgct
      721 tgcgtccgga gtgttgtgag gccggccagc gcgccggtga tgagggtgcg gcggtcaatg
      781 tgtttcattg acatctatac taacgcgcag ggacgggaga caaacaatgc gaattttgca
      841 gatcgcgctg gtgctgggcg tggcggcggc ctcggctggg gcagcgcggg cggcgcaacc
      901 cctgccggaa atcgtgactc gcgacgggcg ttacgcactc attgtggatg gtgcgccgta
      961 cctcgtgctc ggtgcgcagg tgaacaattc gagcaactgg ccggccatgc tgcccaaggt
     1021 ctggccggcg gtgaaaaagc tgcaggccaa caccgtgagc gtgcccattg gctgggagca
     1081 ggttgaaccc gtcgagggaa gattcgattt ctcctttctc gacacgctgt tggccgaggc
     1141 gcgcgaaaac cacgtgcgcc tggtgctgct ctggttcgcc acctggaaga acaatggccc
     1201 taactacgcg cccgagtggg tgaagctcga caacgcgcgc ttcccgcggc tggtcgacgc
     1261 caggggcaaa gtcttgaatt ccatgtcgcc gcattcggcc gccatgctcg ccgccgacaa
     1321 gcgcgcgttc gtcgcgctga tgaagcatct gaagagcgcc gatgaagcgc gcaccgtgat
     1381 catggtgcag gtgcagaacg agcccgggac ctatggcagc gcgcgggatt tcggtccggc
     1441 ggcgcagaag ttgttcgatg ggccggtgcc cgcggcgatc atcaaggcgc agggcgcgaa
     1501 gccgggcacg tggacccagg cattcggcaa ggacgccaat gaatactttc aggcctgggc
     1561 ggtggcgaaa ttcatcggcg agatcgccgc cgcgggaaag aaggagtatc cgctgcccat
     1621 gtacgtgaac gcggcgctgc gcgatccgtt tcatccgggt aacgcgggcg attacgccag
     1681 cggcgggccc accgacaacg tcatatccat ctaccgcgct gccgcgccgg ctatcgacat
     1741 cgtggccccc gacatttaca tgaaggagtc gaacaaggcc tatcgcgtta tcgaactcta
     1801 caagcccggt ggaccgttgc tggtgccgga gatcggcaat gacccgttgt ttgcgcgtta
     1861 tttttacgac gtgctgggcg cacaggcgat cggcatcgtg ccttttggca tcgattacac
     1921 cggctattcg aatttcccgc tgggcgcaaa agacgtcgat gcgggcatcg acgcgttcgc
     1981 gcggccgtat cgtttgctgg ctcccatggc gcgcgagtgg gcgcgcctga gcttcacgaa
     2041 caaggtgtgg ggcgcatcgg aacccgacga tcgatcgagc cgcaagcttg atctcggccg
     2101 ctggcaggcg acgttgagtt tcaacgagtg gcagttcggc atgaaggagt ggttcggcga
     2161 tctgaaggac aagccggagt ggtcgggtgc tccttcgggc ggcgcgctgc tggtgcagct
     2221 aggcgccgac gaattcctgc tcaccggcca gcacgtgcgc gtgtcattcg cgccggccgc
     2281 aggtcgcaaa gcgaacggac tgattttcgc ttcggtcgaa gagggcagct acgtcgacgg
     2341 caagtggtcg cgcacgcgcg tctggaatgg cgatcagact gactacgggc tcaatctcac
     2401 cgacgaaccg cgcgtgctgc gcgtgagact ggcgacgtac tagatcatcc acccaccgtc
     2461 gatcacgtgg atctggccgg tggtgaatcc ggattcgtcg ctggccaggt gcagggcgag
     2521 cgcagcgatt tcttccggct tccctaaacg cccaatcggc tgccgcgcct cgaactcggc
     2581 acgcgtcctg gcgtagtcgc catacgcgtg cagccgtgcc tgcagtgacg gtgtgtccac
     2641 ggtgccgggg cagatggcat tgcagcgaat gccctgcgcg acgaagtccg cggcaacgga
     2701 cttcgtgagc ccgatcaccg cggccttggt ggcgccgtag gcgcagcgat ccggaacgcc
     2761 ggtaatcgaa ctcgccactg acgacatgtt gatgatggag ccgctgcgcg cctcgagcat
     2821 cgccggcaga aacgcgcgga tcatggagaa catggaatgc acgttgagct cgaatgccgc
     2881 ggcccattgc tccggcgtca cggtgaggac ggttcctccg ggcacgatgc ccgcgcagtt
     2941 gaacagcaca tccaccttgc ctgtggcctt cgcgaaggcg gcgatggcaa ccgggtcgcg
     3001 aacgtcgagc acctgtccct cacaacccac gacattcttc agcgcgtttc cgtcgatgtc
     3061 ggcggcgatg acccgcgctc ccgcgcgcgc gaaggcttcc gcgctggcgc ggccgattcc
     3121 cgacccggcc gcggtgacca ccgcaatctt gccggcaagt cgcttagatt ccataggttg
     3181 ctcgtgctgt tgcggagtag atctgttcgc gctcggtggt cttgagcttc gccaacaagg
     3241 tgtcggtgac atcgcgccat tcaccgtacg agcacacgag ctcgcacacg ggccagtcgc
     3301 tgccccacat cattcgtgcc gggccgaagg cgcctagcag atgctctacc gaggcttcga
     3361 gcgccgcggc attcgcgctg cccgcttcgg tgaccaggcc cgagagtttg caggtcatgg
     3421 gcgaggatct tgcgagctcg gtgatcgcct gcttccaggg ttcgatgtcg ccagcggcga
     3481 tcggcggttt ggcgccgtga tccagcacca tgaccagatc cgggtaacgc gccgtaagtt
     3541 cgagcagcgc cggaagatgt ttgggtaata ccaacgcatc gaacacgagg ccatggtcga
     3601 tcatggcctc gaaggccggc gccagttttt cttccagcat ccactcggtg tccgggatgt
     3661 cctggatcat gggccgaagg ccgaccagcg cggggtcggc tgcgagtgtg gcaatggttt
     3721 ccaccacgtc ttcactttca aaatccaccc agcccacgac gcccgcgatg aatgaatgat
     3781 cgcgcgcgag ctcgagcatg aagcgcgttt cggcgacggt ggccgccgcc tgcacgagga
     3841 tggtcgcgcc aacccccgct tccgcgagat gcggcgcgag atgttccggc aggcagtccc
     3901 gatagatagg cgtcaacgcc ggcgtcagcc agccgtagtc accgcgctcc aggcgccaga
     3961 agtgctgatg gctgtcgaca cgggtgctca tggaaacatt catacgggca acggcgcgcg
     4021 cacgtcaacg agaccgcgtt cccgcaacgc gcgccagaac ccggacggta tgggatgtga
     4081 atacatggcg gcgcaattgc gtgcttccgc gccattggcg catccggcca cgacgctggc
     4141 gatccgcgga tgccccaacg ggaactgcag cgccgcggct tgcagcggta cggagaattc
     4201 cccgcaaagc tgttccagcc gggtcacgcg ttcgagaacc gcgggcggtg gtgcggcgta
     4261 gttgtagtgc gtcttcgcgc ccgcgcgcga accggcagcg agaatgccgg agttgaacgg
     4321 acctccgcag atgatggaga cgcggcgctg ttcgcacagc ggcaagagcg tttccagcgc
     4381 gggctgctcg agcagcgtgt aacggccggc gagcaggatg cagtcgagct cgcagtcagc
     4441 gagcagctcg acgcagacct cccattcgtt cacgccgagc cctatggcgc gcaccgtgcc
     4501 ggcgtcgcgc aattcgcgca tcgcgcgata gccaccttcg agaaactccg cgactcgcgc
     4561 ggcgtgcgcg gtgccatgcg tcatgtggcc gatatcgtgg cataacagga tgtcgatgcg
     4621 cgcaaccttg agacgctcga gactctcggc atgcgaacgc atgaccgatt cgtagctgta
     4681 gtcgaacacc ggtgcgaacg gccgtggaga gaagtaaccc tcgcggcctg cgctcgcatc
     4741 ctgcggaccg gtgggtacga ggcgccgtcc aaccttggtt gaaatcaccg gcagagcctg
     4801 cgcgccctgc agggcctgtc ccagtcgtat ttcactgagt ccgaagccat agaacggtgc
     4861 ggtgtcgaag tagcgaaccc cggcggcgaa agattcgcgc accgcggcgc ttgcctcatc
     4921 gtcggggagc tcacgataca ggttgccgat ggcggcgccg ccgaatccga gctcggtgac
     4981 ttcgagcccg ctgtttccca gctcgcggcg cgcgagcgtc atgcgattgc cgtcaccgtg
     5041 tcacgccgcg acttccacgc aaacaggaac acgaccgcga aacagactgc cggcacgata
     5101 taggccaggt tgattgtgct ctcccgcgaa atgaatccca tcgcggccgt gaacacggcg
     5161 ccgccgatga tggccatcac caggaacgac gaccctgact tggtgtagac accgaggccg
     5221 cgcaggctca tcgaaaagat ggtgggaaac atgatggaca tgaagaagct cgacgcgatc
     5281 agcgccgtga ggccgacatg cccgcccgag actaccgcaa tcaggcacag ggccacgttg
     5341 atggccgcga agctggctaa caacttcgcc gggtttatac gcgacatcag cagtgtgccg
     5401 atgagacgac cggcgaagaa aatgccgaag gagatcggca accagcgcag cgcatccttt
     5461 tccagggtgc ctggaaaatt gtgctgcgtg taacggatca ggaagctcca cacccccacc
     5521 tgcgcgccca cgtacgcgaa ctgcgcgagc acgcccagcc agtagcgcgg aaatttcccg
     5581 aggccgccga agccgccttc cgcctcgccg gtgtcatggc cgcgtccgcg gtccaccggc
     5641 gggaattttg cgcaggcgac cagcacggcc cagagaatca ccatggcggc aatcaggacg
     5701 tagggtagtt tgaccgaatc gagctcggtg gcgcggaatg cggcgaaggc atccggcgtc
     5761 aacgcctgca gctgctcggg cgagtagtgc acgtcggaga agatcagcat tccgccgaca
     5821 tacgatccca tcatcgtgcc gatgggattg aacgtctgcg cgaagttcag gcgctggtcg
     5881 gcagtgccgg cgggacccat gacggcgatc agcgggtttg ctgaagtctc gagaaacgcg
     5941 agaccggagg cgatcacgta cagggcgatg aggaatgcgt agtactggct ggcctgcgct
     6001 gcgggcagga acaggagcgc accggctcca tagaggccga ggcccatgag cacggctgcg
     6061 cgatagccga accgtctcat gaacagcgat gccggaatcg caaagcagaa gtagccgaag
     6121 tagaacgccg actgcaccag gctcgatgcg aagtcgtcga gctggaacgc cttgcggaat
     6181 tgcggaatga gcacgtcatt gaggctgttg gccacgcccc acatgaagaa gaggccaacg
     6241 atgagaatcg tcgcgaaaag aaatccgggg gtgatgaacg ccgctttcgg tttcgccggc
     6301 aacgcggcgt cgttcacgga aggtccccat gcacgatttc gttggatatt tgtttagccg
     6361 ctgcccgcac gaactccacg gcatgggcca tctcgacctg caggggattg ctgtgcacga
     6421 acggaatggt gagcgcggcc accgcggtgt cgtcgcgcag gatgggcgcg gacaaatcga
     6481 cgatgccacg tacgaagtgc gacgccgctt cgtggtatcc cttcgaacgc acgatgttgg
     6541 ctcgttcgac gaagctgtcc gcggcctcgc ccttgagccg gcagcgcttg agccagatgc
     6601 ggcggacatc gtcattctga taggcgaaca gcacgaggcc cgaagtcgcg ctcgcgattt
     6661 cgcgccgata accgatgcgc accgagaagc cgaggtctcc cgggcgttcg atgcgcgtga
     6721 tcacgacgat ctgatcttcc gatgccaccg cgatatgcgc cgactgcccg atgtcctgca
     6781 ccagtttgcg catgatgggc agcgccgttt cgaccaggga ccgaaccggc gcctgggcca
     6841 tggccagcgc gaacaacttg ttggtgagga catacccgct gccgttttcc gcagtggtga
     6901 tgaagccttt gaattcgagc acctggagca tgcgaaaaag ctcgcccgat gagcggccca
     6961 ggcgctgcga gatggacgag aggttcaacg gggacttttc agctgccagc agttcgatga
     7021 catcgaggcc cttttcgagg gccggggcac gatatcgact ggtgcgcgga gcggtttcct
     7081 gcgccgggag gcgcggggga gcggtttttg tggccatgga ttctgtagtt gtagtggtaa
     7141 tgaccggcgc tggattctgc cccaaaacca cttgatcttg aattttcatt agagcattgt
     7201 actttcatcc gggaaatcgc gggttgcagg ccatgcagct gcgacgtgcc ggaaggcgag
     7261 actcccgcgg cgcccccacg acgcgaaggc tcgccttccg gcagtttcgc ttttggctgc
     7321 ctcttcggac cggggtctca ggtctcgtta gaattcccgg gcccaaataa ttcgaatcgt
     7381 gtccagaggg gtcccatgaa gatgctgacg agatggatgt gcgtgctcgt gctcgcaggt
     7441 tgtgcagccg aggcgccgga agggtcgttc gtccagaacg aaaccggcat cgtggtgacg
     7501 ccggctgacg gcgccagtcg ccgtgtacgc ctcgaagtca aaaccgaccg tatcgtgcgt
     7561 gtgacgtcgg tgaccgatgg gaacctcgac ctgcccaaga gcctcatggt ggtcgactcc
     7621 ccggcgaagc cggtggcatt ccaggtggag aggcgggaca gcgacgtcgt gctcaaaacc
     7681 tcgcagattg ccgcgcacgt gtcgctcgcc aacggcgcgg tgagtttttc cgatctcgcc
     7741 ggcaaaccgc tgctggccga agagccgttc cgcaagctgg agtccggagt cacacagcgc
     7801 ttcaacgctg gcaccgacga agccttttat ggctcggggc agcatcagaa cgcgcagatg
     7861 aacctgaatg gcgaagacgt cgaactcgcc cagcacaaca tggacattgg cgtgccgttc
     7921 gtggtgtcga cgcgcaacta cggcgtgctg tgggacaaca atggcatcac gcggctcggc
     7981 gacccgaaac cttatggact tgcttcgcgc gacctcagga tccgcgacgt ggcgggtaag
     8041 gacggcggct tcacggcacg ttattacatc gacgggaagc aaaagatcga gcgcgtcgag
     8101 aaggacatca actaccagta catccgcgac cgcttcaact ggcccaagga actgctggtg
     8161 gcgaaggagc cgaacaccgg ctcgccgccc aacatcctcg ccaatcagac ggtgacctgg
     8221 gaaggcacgc tggaatccgc gaaggccggc aaacacaagt tccagctcta cggcagcagc
     8281 tacttcaagg tgtatgtcga cggccggctg gtgatggatc gctggcgcca gaactgggcc
     8341 gcctggtatc acaacttcga tgtcgtcatg acggccggaa ttccggtggc cgtcaggatc
     8401 gagtggattc cgaacgatgg ctacctggcc ttgctgcaca acgacccgct gccggatgcc
     8461 gagcgccact cgctcacgtt cacgtccgac gccgccagcg ccgtcgacta ttacttcatc
     8521 gccggcgcca gccaggatga agtgatcgcc gggtatcgcg cgctcaccgg caaggccgtg
     8581 atgctgccgc gctgggcgta cggcttctgg cagagccgcc agcgctacac cacgcagaaa
     8641 gagttgttgg acgtggtggc cgaataccgc aagcggaaga tcccgctcga caacatcgtg
     8701 atggactggt tttactggaa ggaagaccag tggggttcgc accagttcga caagagccgg
     8761 ttccccgatc ccaaggccat gctcgagaaa gtgcacggca tgaacgcgca cttcatgatt
     8821 tcggtgtggc cgaagttcta tcccggcacc gacaactata aggagctcga ttcggcgggc
     8881 ttcatgtatc gcggcaacat cgaggcggga gcgctcgact gggtgggcaa ggggtaccag
     8941 aactcgttct acgatccgta ctcgaaaccc gcgcgcgaca tgtactggcg ccagctcaac
     9001 gagaacctga acgtgctcgg catcgatgcc tggtggatgg atgccaccga gcccgacctg
     9061 cactcgaatc tcgacatcga ttccatcaag gcgcgcatcg gccccacggc aatgggaccg
     9121 gccgagaagt tcttcaactc ctatccactg gtgcataccg gcggcgttta tgaaggttca
     9181 cgcgtggtga gc
//
LOCUS       DASQJS010000101        14114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_366143, whole genome shotgun sequence.
ACCESSION   DASQJS010000101 DASQJS010000000
VERSION     DASQJS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14114
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366143"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VEW08_04015"
     CDS             complement(<1..299)
                     /locus_tag="VEW08_04015"
                     /inference="COORDINATES: protein motif:HMM:NF020471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HYJ39931.1"
                     /translation="MIFAGQSRDQLRETYRAAWRRYKQQHTLSPLDKQVVAVISEHPE
                     YHVIVESAAADLANYSPRSGQLNPWLHMGLHLAVREQVSTNRPHGIADVHAKLSK"
     gene            complement(296..955)
                     /gene="nth"
                     /locus_tag="VEW08_04020"
     CDS             complement(296..955)
                     /gene="nth"
                     /locus_tag="VEW08_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYJ39932.1"
                     /translation="MNAAKRREIFERFRAANPHPTTELEYETPFELLVGVILSAQATD
                     KSVNAATRKLFPVARTPEAIAKLGVDGLAKYIRTIGLWRTKAKNVIATASAIADEHAG
                     VVPRTREALEKLPGVGRKTANVVLNTIFGEPTIAVDTHIFRVANRMRIAPGETPREVE
                     DALIRFTPGEFAHDAHHWLILHGRYVCKALKPECARCLVNDLCEYPLRRKIIAAARKA
                     P"
     gene            complement(952..1509)
                     /locus_tag="VEW08_04025"
     CDS             complement(952..1509)
                     /locus_tag="VEW08_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HYJ39933.1"
                     /translation="MAKEITAQAIDQLLPQTQCTRCGYTGCLPYAEAIARGEADINQC
                     PPGGTETIVALVRLTGRSLSPLNRDNGIEAAPSIAFIDEERCIGCTKCLPPCPVDAIV
                     GAPRRMHTVIAELCTGCELCVAPCPVDCIVMIPLLKSPLPSLVLPTAGDSRARYESHN
                     QRVARRAADRAATLAAKKRGARPAP"
     gene            complement(1494..2528)
                     /locus_tag="VEW08_04030"
     CDS             complement(1494..2528)
                     /locus_tag="VEW08_04030"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HYJ39934.1"
                     /translation="MPHPDSGGVLARAGLALLHRLPPEASHALGLRALQLTRHWWNSP
                     ATSPALELRILGQHFAHPVGLAAGFDKNGDYLDALGALGFSHVEIGTVTPRPQSGNPK
                     PRLFRARDSRALINRMGFNNKGADHVAAQLRRASYRGIRGVSIGKNAGTPLGRAADDY
                     VDCQRKLYAVADYFAINVSSPNTQGLRELQAADALAGIVEPLRIEGRQLAARHGRQVP
                     LLVKISPDLSSEELSSLCRALVHHCIDGVIATNSSTAIELPDPGMSQTGGVSGAPLAA
                     RSLQVLTELRRELGAKFPIISVGGVMSAADATARLAAGANLVQLYTGFVYRGAPLLAE
                     ILQALEWPKK"
     gene            complement(2537..4588)
                     /gene="metG"
                     /locus_tag="VEW08_04035"
     CDS             complement(2537..4588)
                     /gene="metG"
                     /locus_tag="VEW08_04035"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HYJ39935.1"
                     /translation="MPRKILVTSALPYANGALHLGHIIETVQTDIWVRFQRMQGHDCI
                     YVCAEDTHGTPVMIKAQAEGITPEELITRVAAEHRADYEGFLIGHDHFHSTHSAENKA
                     ITADLYGKLKAAGHITTRSVRQAYDEKAGMFLPDRYVKGECPVCHTADQYGDSCENCG
                     ATYTPDKLISPVSTISGTPPVWRESEHYFFKLGDFTAVLRGWLEGGGARQPEVRAKLA
                     EWFEAGLQDWDISRDAPYFGFEIPGAPGKYFYVWFDAPIGYLGSFKALCDKRGLNYDE
                     YLKADSTTELYHFIGKDISYFHNLFWPAVLHGSGCRKPTAVFVHGFLTINGAKMSKSR
                     GTFITARRYLERLPPEPLRFYFAGKLSSGVDDIDLSLEDFVARVNSDIVGKLVNIASR
                     CAGFVQRGGGKLAAQLPDPQLYAEFAALRSTIGDLYDARDYSAAMRGIMGLADRANQY
                     VDTHKPWALAKDPARAAEVLAVCTQGINLFRVLMSYLAPVMPQMAEKAGKFLRISFAD
                     WNSVDTPLFDHPIEAYEPLATRLDPKVVAELVEATDAAPIKPAEKVKKPVTTPPASAS
                     AATPANNSGLVNIGDFAKLDLRVAKVLDARLVEGSDKLLQLTLDLGAEQRNVFSGIRS
                     AYDPATLIGRHVVMIANLEPRKMRFGVSEGMVLCASPDSGSEVFLLSADSGAQAGMKV
                     T"
     gene            complement(4639..5700)
                     /locus_tag="VEW08_04040"
     CDS             complement(4639..5700)
                     /locus_tag="VEW08_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HYJ39936.1"
                     /translation="MPVIDEYLKEVLGRKGSDLHFLAGDPPRIRLYGDLQPLRPEKLE
                     KEFVQNALYEIMPKTAAMRFEEHDGADFAYNMGEYGRFRVNVMRQLNGMGAVMRAIPS
                     KALSMEQLNLPQACHTLCRANNGLILVTGKTGSGKSTTLAAMIDDINSRLKGHILTIE
                     DPIEFVHARKQCLISQREVGVHTKGFASALQSGLREDPDVVLVGELRDYETISIAVTA
                     AEMGILVMGTLHTNGAAPTVDRMINVFPADKQSHVRTMLSTSLRGVISQQLLQKADKS
                     GRVAALEILVNTPAVSNLIRQGKLDQLETTMQSGSQFGMRTMDNAIEALLNQRLITGK
                     EAYKKGISKAKFEPMKDQG"
     gene            complement(5709..6827)
                     /locus_tag="VEW08_04045"
     CDS             complement(5709..6827)
                     /locus_tag="VEW08_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HYJ39937.1"
                     /translation="MARIDAFLKLGMQQGCSDVHLAVGVPPMLRMNGDLMPIKFRDVG
                     DSELESYLTEIFTKNQHEYFGKGNDLDFSYVSADGGRFRVNVFRKETGLGATFRAIPQ
                     DIPSIDKLALPPIVKKLCDYHQGMVLVTGATGTGKSTTLAAMIDHLNTTRRLNIVSLE
                     DPIEFVHRSKLSQVIQRELGTHIPSFAEGVRAAMREDPDVILVGELRDAETISMAMTA
                     AETGHLVLGTLHTTSASKTIDRIIDALPIEEREQTKSFLSQSLLAVVTQILVKTHDQR
                     ARKAICETLVMTRAIAKLIQTDQTHQIPNQIQTGKDLGMQLLDQALLHSLAAKEIDPD
                     DAVTYASDRRPFQRYVTDTTLMPKIDVTGPATASGGSG"
     gene            complement(7002..9557)
                     /locus_tag="VEW08_04050"
     CDS             complement(7002..9557)
                     /locus_tag="VEW08_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HYJ39938.1"
                     /translation="MSFFTNIRADRFITELRSATDVAAPATQKAIAKLRELGPGAIEP
                     VTEALADADKIATVAYIEVLTGLVNQKTLPKFLEAMVTGSPRVVAGVAWALSSSRAYP
                     PTMLLEALGTEGIAKSALLDVITAHRSRLSAREILAAAYKQEANEKAALFRILGELAT
                     DNDLPELVGRLNGKDPVARLHLINILSRFPKPEVQRALQQQLKDPNKLVRAATLGALA
                     KMDGPIEIEQVCLLLRDPEIDVQNKAIDVVIRANDPDTIKYLIPVLKDENEYARRAAV
                     EVLNEIGNAKSVKELLEAVADDDWWVRSRAADALGKIGGPKVVEAVLQLVGDPDEEIR
                     RAAVEILNQTKDDRAVGHLIDATKDKDWWVSERAVDALAEIGSKRAVPRLLEMLGTTP
                     PRSLPVVVRALGRLGDHKVIDSVMPMLAREEKDIRLEAISSLAKLADERRLENIRVQI
                     QAQTSATEQTVAQAALRALSDLDSRFSHSGTRLSSTQNRSPLAQPGGRPASATQASST
                     PAMASSPSVPQGGRPRTVAPPPAPPPTSPPPVHASTSKEARTQLMSEQDVQRAVKEAE
                     KMAGPQKLDIQTLKPGDIIEGRYKYVERIGKGAFGTVLLMEDTVVDERLILKFLNPNV
                     SEDEEIMKRFVHELRYSRKITHHNVIRIYDFLFIQGNYAISMEYFPSHTLGSEVVGEK
                     PMPLARALGFGIDICTGMEVAHAQGIVHRDLKPANVLINNEGLLKIVDFGVAAAQREG
                     DTQLTKTGYVIGSPKYMAPEQILGKKVDQRADVYALGVILYEIMTGVPPYARGDHMSV
                     MYQHVQGKARPPREINPALPPGLPEVIMQAMSVDKDKRYQTMPLLREALEKFR"
     gene            9767..10861
                     /gene="apbC"
                     /locus_tag="VEW08_04055"
     CDS             9767..10861
                     /gene="apbC"
                     /locus_tag="VEW08_04055"
                     /inference="COORDINATES: protein motif:HMM:NF008669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HYJ39939.1"
                     /translation="MALNAAAEFRALVEEYPDPFLRQTLGSAQAVDLAELKGDRGHVR
                     VALGFPVGGYEDEFKRGLEAHLATRGGPSEIQVELVQKITAHAVQRQLSPQAGVRNIV
                     AVASGKGGVGKSTVAANLALAWVKQGARVGILDADIYGPSQPIMLGLTGQRPASPDGK
                     RIKPLMAHGLAAMSIGFLIDPEQPMVWRGPMVTQALTQLLGDTDWGDLDYLVIDMPPG
                     TGDIQLTLAQRVPVAGAVIVTTPQDIALADARKGLKMFEKVNVPVLGVVENMSVHVCG
                     NCGHVEHLFGAGGGARMAAQYNVELLGELPLDARVREEADGGVPTVVAAPDSPHAAPY
                     FIMARRTAAALARRSKDRSTLFPKIVVEES"
     gene            10861..11430
                     /gene="dcd"
                     /locus_tag="VEW08_04060"
     CDS             10861..11430
                     /gene="dcd"
                     /locus_tag="VEW08_04060"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HYJ39940.1"
                     /translation="MSIKSDHWIRRMAAKEKMIEPFAPEQVRAAADGHRIVSYGTSSY
                     GYDVRCSREFKIFTNINSTIVDPKNFDEKSFVDVHADVCIIPPNSFALARTVEYFRIP
                     RSTLVVCLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYANEGVAQMLF
                     FESDEVCSTSYKDRGGKYQGQTGVTLPKT"
     gene            complement(11521..12225)
                     /locus_tag="VEW08_04065"
     CDS             complement(11521..12225)
                     /locus_tag="VEW08_04065"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HYJ39941.1"
                     /translation="MRLPERFLPLLLLAAGLAAHADPVDLKPFRASYIAEWKGVTAAS
                     STVELKNISTDIYTYSSVNSARGIFRMAFPDALSQKSTFRIINGRVEPMLFEGSDEKE
                     RPINLTFDWQKKRVTGVAKERAVDLELPDGAQDAMSLQIASLRNLASGDLKGTVWMID
                     ATKLKEYELHLEGNARIQTELGELDTIVYTSKRQSSDNYTRTWVAPALGYLPVRAERI
                     DGKKVLITLLIQSVDP"
     gene            complement(12212..12874)
                     /gene="purN"
                     /locus_tag="VEW08_04070"
     CDS             complement(12212..12874)
                     /gene="purN"
                     /locus_tag="VEW08_04070"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HYJ39942.1"
                     /translation="MTGLPMPVAVVISGRGSNMEAIARASLAPGSPFRIVRVIADRDS
                     AGGLARAAALGIPTSIVPVKQFTDRASFDAALAAELDASGARLVALAGFMRILSAEFV
                     QRFEGRLLNIHPSLLPKYKGLDTHARALAAGDSHHGASVHYVTAELDGGPVIMQGRLR
                     IHPADTSDTVSARVHTLEHIIYPHVCTLIGAGRVEWRAGSVFFDGAPLTAPLIEENDA
                     AA"
     gene            complement(12871..13902)
                     /gene="purM"
                     /locus_tag="VEW08_04075"
     CDS             complement(12871..13902)
                     /gene="purM"
                     /locus_tag="VEW08_04075"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HYJ39943.1"
                     /translation="MTETGLTYRDAGVDIDAGDELVERIKPLVKRTMRKEVLAGIGGF
                     GALVEVPLDRYRRPVLVSGTDGVGTKLRLAIDTGRHDKIGIDLVAMCANDIVVSGAEP
                     LYFLDYYATGKLRVDVAEAVIRGIAEGCELAGAALVGGETAEMPGMYHGDDYDLAGFC
                     VGVVERDRIIDGTKIAAGDVVIGLASSGPHSNGYSLIRKLVSVAGADDKTQLDGKTLF
                     DRLLTPTRIYVKSLLSLAQKISVKGFAHITGGGLTDNIPRVLPEGFDVALHRRNWPRD
                     PVFDWLAATGRISAPEMYRTFNCGIGMVVIVAPADVDAALAHLKASGETAWIVGGVGS
                     GSRGVVIEE"
BASE COUNT         2365 a         4274 c         4748 g         2727 t
ORIGIN      
        1 tgtttggaca gcttcgcgtg gacatcggcg atgccatgcg ggcggttggt ggaaacctgt
       61 tcgcggacgg cgaggtgcag tcccatgtgc agccagggat tgagctgtcc gctgcgcggg
      121 gagtagttag cgagatcggc cgcggccgac tccacgatca cgtggtattc cgggtgctcg
      181 gagatcaccg ccaccacctg cttgtcgagc ggcgagagcg tgtgctgctg cttgtaccgg
      241 cgccacgcgg cgcgataggt ctcgcgcagc tggtcccggg attggccggc gaatatcacg
      301 gagccttcct ggccgcggcg atgatcttgc ggcggagcgg gtattcgcat aaatcgttca
      361 cgagacaacg tgcgcattcg ggcttcaatg ccttgcagac ataacggcca tgcaggatga
      421 gccagtgatg tgcgtcatga gcgaattccc cgggcgtgaa cctgatcaat gcgtcttcca
      481 cttctcgcgg agtttcgccc ggggcgattc tcatgcggtt ggcgacgcgg aaaatatgag
      541 tgtccactgc tattgtcggc tcgccgaaga ttgtattcag taccacgttc gcagttttgc
      601 gacccacgcc cggaagtttt tcgagtgcct cgcgagtgcg cggcacaacg cccgcatgtt
      661 cgtcggcaat cgccgacgcg gtggcaatca cattctttgc cttggttctc cacaggccta
      721 tcgtgcggat gtatttcgca agcccgtcga cgccgagctt cgcaatagcc tctggagtac
      781 gggccaccgg aaacagtttg cgcgttgcgg cgttgacgct cttgtcggtg gcctgcgccg
      841 agaggattac cccgaccagc agctcgaacg gtgtctcgta ttcgagctcg gtggtgggat
      901 gcggattcgc agcgcggaat cgctcgaaga tctcgcggcg cttggcggca ttcatggtgc
      961 aggccgcgcg ccgcgtttct tggcggcgag tgtcgcagcg cgatcggctg cacgccgcgc
     1021 gacgcgttgg ttgtgagact cgtagcgggc acgcgagtcg cctgccgtcg gcaggaccaa
     1081 tgacggcaac gggctcttga gcaaaggaat catcacgatg cagtccaccg ggcatggcgc
     1141 gacgcagagc tcgcaacccg tgcagagttc cgcgatgacg gtgtgcatgc gccgcggcgc
     1201 gccgacgatg gcatccacgg ggcagggcgg caggcacttg gtgcagccga tgcagcgttc
     1261 ttcgtcgatg aaggcgatcg agggcgcggc ctcgatgccg ttgtctcgat tgaggggcga
     1321 cagtgaccgg ccggtaagac gcaccagggc gacgatggtc tcggtgccgc ccggcgggca
     1381 ttgattgatg tccgcctcac cacgcgcgat ggcttccgca taaggcaggc aacccgtata
     1441 gccgcagcgc gtgcactggg tctgcggcaa gagctgatcg atggcttgag ctgttatttc
     1501 tttggccatt ccagcgcctg caggatctcg gccagcaagg gggcgccacg atatacgaag
     1561 ccggtgtaga gctgcaccag gttcgcgccg gcggcgaggc gcgccgtggc atcggcggcg
     1621 ctcatcacgc cgcccacgct gatgatcggg aatttcgcgc ccagctcgcg ccgcagttcg
     1681 gtcagtactt gaagcgagcg tgccgcgagc ggcgcgccgc tgacaccgcc agtttgggac
     1741 attccgggat ccggcagctc gattgccgtg ctgctgttgg tggcgatcac gccgtcgatg
     1801 cagtgatgca cgagcgcgcg gcaaaggctg ctcaattctt cggacgaaag atccggcgaa
     1861 atcttcacga gcagcggtac ttgccggcca tgtcgcgcgg caagctgccg accttcgatt
     1921 cgcagaggct caacgatccc ggccagcgca tcggcggcct gcaattcgcg taaaccctgc
     1981 gtattcggcg acgacacatt gatggcgaag tagtccgcga cggcgtagag ctttcgctgg
     2041 caatcgacgt aatcgtcggc ggcgcgcccc agcggtgtgc cggcattctt gccgatgctc
     2101 acgccacgga tgccgcgata gctggcgcgg cgcagttgcg cggcgacgtg atccgcaccc
     2161 ttgttgttga atcccatgcg attgatcagt gcgcgcgaat cccgtgcccg gaacagtcgc
     2221 ggtttgggat tgcccgactg aggtcgcggc gtgacggtgc cgatttccac gtggctgaag
     2281 cccagggcgc caagcgcatc gagatagtcg ccgttcttgt cgaaaccggc ggcaagtccg
     2341 accgggtgcg cgaagtgctg gccgagtatg cgcaactcga gcgcgggcga tgtcgccggt
     2401 gagttccacc aatgccgtgt gagttgcagg gcgcgcaggc ccagggcatg cgaagcttcg
     2461 ggcggcagcc gatgcagcag cgccagtccc gcgcgcgcca gcacgccgcc ggaatccggg
     2521 tgcggcacgg ctccgctcag gtgaccttca tccccgcctg cgcgccgctg tcggcggaga
     2581 gcaggaatac ctcggaaccc gaatccgggc tcgcgcacag caccatgcct tcggagacgc
     2641 cgaagcgcat cttgcgcggc tcgaggttgg cgatcatgac cacgtggcgg ccgatgagcg
     2701 tcgccgggtc atacgccgag cggattcccg agaagacatt gcgttgctcg gcgccgaggt
     2761 cgagcgtgag ctgcagtagt ttgtccgaac cttcgacgag cctggcatcc agcaccttgg
     2821 cgacgcgcag gtcgagcttg gcgaagtccc cgatgttcac gaggccggaa ttgttggcgg
     2881 gcgtagcagc cgacgctgaa gcgggaggag tcgtcacggg cttcttcacc ttctcggcag
     2941 gtttgatggg ggcggcgtcg gtggcctcga ccagctcggc cacgaccttc gggtcgaggc
     3001 gggtcgccag aggctcatag gcctcgatcg gatggtcgaa cagcggcgtg tccaccgaat
     3061 tccagtcggc gaacgagatg cgcaggaact tcccggcctt ttccgccatt tgcggcatta
     3121 ccggcgcgag gtagctcatg agcacgcgga acaggttgat gccctgcgta catacggcga
     3181 gtacctcggc ggctctcgcc gggtccttgg cgagtgccca aggcttgtgc gtgtccacgt
     3241 actgattggc acggtcggcg aggcccatga tgccgcgcat ggcggcggaa taatcgcgcg
     3301 cgtcgtacag atcgccaatc gtgctgcgca gcgccgcgaa ctcggcgtac aactgcgggt
     3361 cgggtagctg ggcggccagc tttccgccgc cgcgctgcac gaagcccgcg caacggctgg
     3421 cgatgttcac gagtttgccg acaatgtcgg agttcacgcg cgcgacgaag tcttcgagcg
     3481 acagatcgat gtcatcgaca ccggagctga gcttgccggc gaaatagaac cgcagcggtt
     3541 cgggcggcag ccgttccagg tagcgccgcg ccgtgatgaa cgtcccgcgg gacttcgaca
     3601 tcttcgcgcc gttgatcgtg aggaagccgt gcacgaacac cgcggtgggc ttgcggcagc
     3661 ccgagccgtg cagcaccgcg ggccagaaca ggttgtggaa gtagctgatg tccttgccga
     3721 tgaagtgata cagctcggtg gtgctgtcgg ccttgagata ttcgtcatag ttcaggccgc
     3781 gtttgtcaca caaggccttg aagctgccga gataaccgat gggcgcgtcg aaccacacgt
     3841 aaaagtattt cccgggcgcg cccgggatct cgaagccgaa gtacggcgca tcgcgagaga
     3901 tgtcccagtc ctgcaggccc gcttcgaacc actcggcgag tttggcgcga acttcgggct
     3961 gacgcgcgcc gccgccctct aaccacccgc gcagcaccgc cgtgaaatcg ccgagcttga
     4021 agaagtagtg ttcggactcg cgccagactg gcggtgtgcc ggagatcgtc gagaccgggc
     4081 tgatcagctt gtcgggcgtg tacgtggcgc cgcagttctc gcaggagtcg ccgtactgat
     4141 cggccgtgtg gcagaccgga cattcgcctt tgacgtagcg atcgggcagg aacatgccgg
     4201 ccttctcgtc gtaagcctgg cgcacgctgc gcgtggtgat gtgccccgcg gctttcagct
     4261 tcccgtacag gtcggcggtg atcgccttgt tttcagccga gtgcgtggag tgaaagtgat
     4321 cgtggccgat caggaatcct tcgtagtcgg cgcgatgctc ggccgccacg cgcgtgatca
     4381 gctcttcggg tgtgatcccc tcggcctgcg ccttgatcat gaccggcgtg ccgtgcgtgt
     4441 cttcagcgca cacgtagatg cagtcgtgac cctgcatacg ctggaagcgt acccagatgt
     4501 cggtctgcac ggtttcgatg atgtggccga ggtgcagcgc gccgtttgca tagggcaacg
     4561 cgctggtgac gaggattttg cgaggcatga aactgcggtc cgaaaggggg ccgcaagtat
     4621 agacgggcgc ccgccttctt agccctgatc cttcatgggc tcgaacttgg ccttgctgat
     4681 gcccttcttg taggcctctt tgcccgtgat caagcgctgg ttgagcagcg cctcgatggc
     4741 attgtccatg gtgcgcatgc cgaactggct gcccgactgc atggtggtct cgagctggtc
     4801 cagctttccc tggcggatca ggttggaaac cgccggcgtg ttcaccagga tttcgagcgc
     4861 ggccacgcgg ccgctcttgt ccgccttctg cagcagctgc tgcgagatca caccgcgcag
     4921 gctggtggac agcatggtgc gaacgtgact ttgtttgtcg gccgggaaga cgttgatcat
     4981 gcgatcgacg gtgggtgcgg cgccgttggt gtgcagcgta cccatgacca ggatgcccat
     5041 ctcggcggcg gtgaccgcga tgctgatggt ttcgtaatcg cgcaactcgc cgaccagcac
     5101 cacgtccgga tcttcgcgca ggcccgactg cagcgccgac gcgaagccct tggtgtgtac
     5161 gccgacttcg cgctggctga tgaggcactg tttgcgcgcg tgcacgaatt cgatggggtc
     5221 ctcgatggtg aggatgtgtc ccttgagacg cgaattgatg tcgtcgatca tcgccgccag
     5281 cgtggtcgat ttgcccgacc cggttttccc cgtgacgagg atgagcccgt tgttggcgcg
     5341 gcacagcgtg tggcacgcct gcggcaggtt gagctgctcc atcgacagcg ccttcgacgg
     5401 aatggcgcgc atgacggcgc ccatgccgtt cagctgccgc atgacgttga cgcggaagcg
     5461 cccgtactct cccatgttgt aggcgaagtc ggcgccatcg tgttcctcga aacgcatggc
     5521 cgcggtcttc ggcatgattt cgtagagcgc gttctgcacg aactctttct cgagcttttc
     5581 cggccgcagc ggctgcaggt caccgtagag gcgaatgcgc ggcggatcgc cggcgaggaa
     5641 gtggaggtcc gagcctttcc tgcccaggac ttccttgagg tattcgtcga tgacgggcat
     5701 gagcgcggtt agcccgaacc accggacgcc gtggccgggc ccgtgacgtc gatcttgggc
     5761 atgagcgtcg tgtcggtgac gtagcgctgg aacggccgcc ggtcggaggc gtaagtgacc
     5821 gcgtcgtcgg ggtcgatttc cttggcggcc agcgaatgga gcagcgcctg gtcgaggagc
     5881 tgcatgccga gatccttgcc ggtctggatc tggttcggaa tctggtgcgt ctggtcggtc
     5941 tggatgagct tggcgatggc acgcgtcatc accagggttt cgcagatggc cttgcgcgcg
     6001 cgctggtcat gggtcttcac gagaatctgc gtgaccacgg ctaacaagct ttgcgacagg
     6061 aagctcttgg tctgctcgcg ctcttcgatg ggcagcgcgt cgatgatgcg gtcgatggtc
     6121 ttcgaggccg acgtggtgtg cagcgtgccg agcacaaggt ggccggtttc cgcggcggtc
     6181 atggccatgg agatggtttc ggcgtcgcgc aactcgccta ccaggatcac gtcgggatct
     6241 tcacgcatgg cggcgcgcac gccttccgcg aacgacggaa tgtgggtgcc gagctcgcgc
     6301 tggatgacct ggctcagctt gctgcgatgg acgaattcga tgggatcttc gagcgagacg
     6361 atgttcaggc gccgcgtggt gttcaggtgg tcgatcatcg ccgcgagcgt ggtcgacttg
     6421 ccggtacccg tggcgccggt gaccagcacc atgccctggt ggtagtcgca gagtttcttg
     6481 acgattggcg gcagcgccag cttgtcgatg ctcgggatgt cctggggaat cgcgcggaac
     6541 gtggcgccaa ggccggtctc cttgcgaaac acgttgacgc ggaaacggcc gccgtcggcc
     6601 gacacatagg agaagtcgag gtcgttgccc ttgccgaagt attcgtgctg gttcttcgtg
     6661 aagatctcgg tgagataact ctcgagttcg gagtccccga catctcgaaa cttgataggc
     6721 atgaggtcgc cattcatgcg cagcatgggc ggcacgccga cggccaggtg cacgtcggaa
     6781 cagccttgct gcatgccaag cttcaggaac gcgtcaatac gagccacggg ggcttaggtc
     6841 ctctgtgtta agtctcacca aagcatgcga cggtgtgacg agcgtcaagt gtgacgcagt
     6901 gcgcgattct ccgcgagccg attcggcaat ggcaataccg aggcgaatac tgaggggtaa
     6961 tcctcagctg gcgaagcgcc gcgcccgcga cgcgctgcgg ctcaacggaa tttttcgagg
     7021 gcttcgcgca gcagcggcat ggtctggtag cgcttgtcct tgtccaccga catggcctgc
     7081 atgatgactt cgggcagtcc cggtggcagg gccgggttga tctcgcgcgg cgggcgcgcc
     7141 ttgccctgca cgtgttggta catgacggac atgtgatcgc cgcgcgcata cggcggcacg
     7201 ccggtcatga tttcgtaaag gatgacgccg agcgcgtata cgtccgcccg ctggtccact
     7261 tttttgccga gaatctgttc gggcgccatg tacttcggtg agccgatgac atagccggtc
     7321 ttcgtgagct gcgtgtcacc ttcgcgctgc gcggcggcca cgccgaagtc cacgatcttg
     7381 agcagccctt cgttgttgat gagcacgttc gcgggcttga ggtcgcgatg cacgatgccc
     7441 tgggcatgcg cgacttccat gcccgtgcag atgtcgatgc cgaagccgag cgcgcgcgcg
     7501 agcggcatcg gcttctcacc gaccacctcg ctgccgagcg tgtgcgaggg gaaatactcc
     7561 atcgagatgg cgtagttgcc ctggatgaac aggaagtcgt agatgcggat gacgttgtgg
     7621 tgcgtgatct tgcgcgagta gcgcagctca tgcacgaagc gcttcatgat ctcttcgtct
     7681 tcagagacgt tcggattgag gaacttgagg atgagccgct cgtcgacgac ggtgtcttcc
     7741 atgaggagca cggtgccgaa cgcccccttg ccgatgcgtt cgacgtattt gtagcgccct
     7801 tcgatgatgt cgccgggctt cagtgtctgg atgtcgagct tctgcgggcc cgccatcttt
     7861 tcggcttcct tcaccgcgcg ctgcacgtcc tgctctgaca tgagctgcgt acgcgcctct
     7921 ttcgaagtgg acgcatgtac cggtggcggc gaagtgggtg gcggcgcggg aggcggcgca
     7981 accgtgcgcg gacgtccgcc ttgcgggacg ctgggtgagg acgccatcgc cggtgtgctc
     8041 gatgcctgcg tcgccgaagc cgggcgaccg ccgggttgcg ccagcggcga acgattctgt
     8101 gtcgatgaaa ggcgcgtgcc gctgtgcgag aagcgagaat ccagatccga cagcgcccgc
     8161 agcgcggcct gcgccacggt ctgttcggtc gccgaggttt gcgcctggat ctgcacgcgg
     8221 atgttctcga gccggcgctc gtcggcgagt ttcgcgagcg agctgatggc ctccaggcgg
     8281 atgtcctttt cttcgcgggc cagcatgggc atcacggaat cgatgacctt gtggtcgccg
     8341 aggcgaccga gcgcgcgcac cacgaccggc agtgaccgcg gcggggtggt gcccagcatt
     8401 tcgagcagac gcggcacggc acgcttgctg ccgatctcgg cgagcgcatc caccgcgcgt
     8461 tcgctcaccc accagtcctt gtccttggtg gcgtcgatga ggtgcccgac ggcgcgatcg
     8521 tccttggtct ggttgagaat ctcgaccgcc gcccggcgga tttcttcgtc cgggtccccg
     8581 accagctgca gcacggcctc gacgaccttc gggccgccga tcttgccgag cgcgtcggcg
     8641 gcgcggctgc gcacccacca gtcgtcgtcg gcaaccgctt cgaggagttc cttgaccgac
     8701 ttggcgttgc cgatttcgtt gagcacttcc accgcggcgc ggcgcgcgta ttcgttttca
     8761 tccttgagca cggggatgag gtatttgatg gtgtcgggat cgttggcgcg gatgacgacg
     8821 tcgatggcct tgttctgcac gtcgatttcc ggatcgcgca gcagcaagca cacctgttcg
     8881 atttcgatcg gtccatccat cttggcgagc gcgcccagcg tggcggcgcg caccagcttg
     8941 ttggggtcct tgagctgttg ttgcagcgct cgctggactt cgggcttcgg aaagcgcgac
     9001 aggatgttga tgaggtgcag ccgtgccacc ggatccttgc cgttcaaccg gccgacgagt
     9061 tcgggcaggt cgttgtcggt ggcgagttca ccgaggatgc ggaacagcgc cgccttctcg
     9121 ttcgcttcct gtttgtaggc cgcggccagg atttcgcgcg cggacaggcg agagcgatgt
     9181 gcggtgatga cgtcgagcag ggcggacttg gctattccct cggttccgag tgcttcgagc
     9241 agcatcgtcg gcggatacgc gcggctgctc gacagtgccc aggcgacacc ggccaccacc
     9301 cgcgggctgc ccgtgaccat ggcctcgagg aatttcggaa gggttttctg gttgacgagt
     9361 ccggtgagga cttcaatgta cgcaaccgtc gcgatcttgt cggcatcggc cagggcttcg
     9421 gttaccggtt cgattgcccc ggggccaagc tcgcgcagct tcgcgatagc tttctgggtt
     9481 gcgggagcgg caacgtccgt cgcgctcctg agctccgtga tgaaccggtc ggcccggatg
     9541 tttgtgaaaa aactcatgga tccgatggcc cgctgggcgc gcgacggcca aagccgcgag
     9601 atgcgcgtgc ggagttaagc aagcctgccg tccttatgta gtccgaaccc tattacaaaa
     9661 attatgccac atgtcctgtc gccaacagcc gatctgagac cgcacaacag gtattttcgt
     9721 tagaatcccg gcccctgaag gtcccggcgg acaactcgaa aatcatatgg ccctcaatgc
     9781 tgccgccgaa tttcgcgcac tggtcgaaga atatccggac ccatttctca gacagacgct
     9841 aggcagcgcc caggcggtcg atttggctga gctgaagggt gacaggggtc acgttcgcgt
     9901 cgccctgggc tttcctgtgg gcggttatga agatgagttc aaacgtggcc tcgaggcgca
     9961 tctggcgacc cgcggcgggc cttcagagat ccaggtcgag ctggtgcaga agatcaccgc
    10021 acacgccgtg caacggcaat tgtcgccgca ggctggagtt cgaaacattg tggctgtcgc
    10081 ctcgggcaaa ggtggcgttg gcaagtccac ggtcgcagca aaccttgcgc tcgcctgggt
    10141 gaagcagggc gcccgggttg gaatcctcga tgccgatatc tacgggccga gccagcccat
    10201 catgctcggt ctcacgggcc agcggcccgc gtcgccggat ggcaagcgca tcaaaccact
    10261 catggcgcat ggcctggcgg ccatgtcgat cggctttctc atcgatcccg agcagcccat
    10321 ggtctggcgc ggccccatgg tcactcaggc gctgacccag ctgcttggcg acaccgactg
    10381 gggcgacctc gattacctcg tgatcgacat gcctccgggc acgggcgaca tccagctcac
    10441 gctcgcgcag cgagtgccgg tggccggcgc cgtcatcgtg accacgccgc aggacattgc
    10501 gctggccgat gcgcgcaagg gcctcaagat gttcgaaaag gtgaacgtgc cggtactcgg
    10561 cgtggtcgag aacatgagcg tgcacgtctg cggcaactgc ggccacgtcg agcacctgtt
    10621 cggcgccggc ggtggcgcgc gcatggcggc gcaatacaac gtcgagctgc tgggtgagct
    10681 gccgctagac gcacgggtgc gcgaagaagc ggacggcggt gtgcccaccg tggttgcggc
    10741 cccggattcc ccgcacgcgg ccccttactt catcatggcg cgccgcaccg ccgcggccct
    10801 cgcgcgccgg tcgaaggatc gttcgaccct gtttcccaaa attgtcgtgg aagagagcta
    10861 atgagcatca agtccgacca ctggatccgc cgcatggcgg cgaaagaaaa aatgatcgaa
    10921 ccgttcgcgc cggagcaggt gcgtgcggca gcggatggcc acaggatcgt ttcctacggt
    10981 acgtcgagtt atggctacga cgtgcgttgc tcgcgggaat tcaagatctt caccaatatc
    11041 aactcgacca tcgtcgatcc gaagaacttc gacgagaaga gttttgtcga cgtgcacgcc
    11101 gacgtctgca tcatcccgcc caactccttc gcgctggccc gcacggtgga atatttccgc
    11161 atacctcgct cgaccctagt cgtgtgtctc gggaagtcga cgtatgcgcg ttgcggcatc
    11221 atcgtcaacg tgacgccgct cgagccggaa tgggagggtc acgtgacgct ggagttctcc
    11281 aacaccacgc cgctgcccgc caagatctac gccaacgaag gcgtggcgca gatgttgttc
    11341 ttcgaatccg atgaagtgtg ttcgacgtcg tacaaggatc gtggcgggaa gtaccagggc
    11401 cagaccggcg tgacgctgcc gaagacgtga tcggtgccgg gccggattgg agtccgacaa
    11461 tgcggcggag cgggttcggt ggcgcgatgg ggaagttttc ccggtttccc ggcccggcac
    11521 ctacgggtcg acagactgga tcagcagcgt gatcagcacc ttcttgccgt cgatgcgctc
    11581 ggcacggacg ggtagatagc cgagcgcagg cgcgacccag gtgcgcgtgt agttatcgga
    11641 gctttgccgc ttgctcgtgt agacgatggt gtcgagttcg ccgagctcgg tctggatgcg
    11701 ggcgttgcct tccagatgca gctcgtattc cttgagcttg gtggcgtcga tcatccacac
    11761 cgtgcctttg aggtcgccgc tggccaggtt gcgcagcgag gcgatctgca gcgacatcgc
    11821 gtcctgcgcg ccatcgggaa gctcgagatc gacggcacgt tccttggcga cgccggtgac
    11881 gcgcttcttc tgccagtcga acgtgaggtt gatggggcgc tctttttcgt ccgagccctc
    11941 gaacaacatc ggctcgacgc ggccattgat gatcctgaag gtgcttttct gcgacaacgc
    12001 gtcgggaaac gccatgcgga agatgccgcg cgccgagttc accgatgaat aggtgtagat
    12061 gtccgtcgag atgttcttca gttccaccgt cgagctcgcg gcggtaacgc ctttccattc
    12121 ggcaatgtag ctggcgcgaa acggcttcag gtcgacggga tccgcatggg cggcaagacc
    12181 ggcggccagc agcaacaggg gcaggaaacg ttcaggcagc cgcatcgttt tcctcgatga
    12241 gtggcgcggt gaggggagcg ccgtcgaaaa acaccgaccc ggcgcgccat tcgacgcgtc
    12301 cggccccaat gagggtacaa acatgagggt agatgatgtg ttccagcgta tgaacgcgcg
    12361 ctgaaaccgt gtcagacgtg tccgctgggt ggatccggag gcgcccctgc atgattaccg
    12421 gccccccgtc gagctcggcg gtgacgtaat ggacgctggc gccgtggtgc gaatcacccg
    12481 ccgcgagcgc gcgggcgtga gtatccaggc ctttgtactt gggcagcagc gaggggtgga
    12541 tgttgagcag gcggccttcg aagcgctgca cgaactccgc cgaaaggatg cgcatgaaac
    12601 cggcgagcgc caccagacgt gcgccgctcg catcgagctc tgccgccagc gccgcgtcga
    12661 aactggcccg gtcggtgaac tgtttgacgg gcacgatgct ggtcggtatg ccgagcgccg
    12721 cggcccgcgc caggccgccg gcgctgtcgc gatcggcgat gaccctaact atcctgaagg
    12781 gcgaaccggg agcgagcgag gcgcgcgcga tggcctccat gttgctgccg cgcccggaaa
    12841 tgaccacggc caccggcatc ggcaggccgg tcattcttcg atgacgacgc cgcgcgaacc
    12901 gctcccgacg cctccaacaa tccaggcggt ttcgccgctc gccttgaggt gcgcgagcgc
    12961 cgcatcgaca tcggctggtg cgacgatcac caccatgccg atgccgcaat tgaaggtgcg
    13021 gtacatctcc ggggccgaaa tcctgccggt ggcggcgagc cagtcgaata ccggatcgcg
    13081 cggccagttc cgccggtgca gcgccacgtc gaagccttct ggcagcacgc gcggaatgtt
    13141 gtcggtgagg ccgccgccgg tgatgtgtgc aaatcctttg acgctgatct tttgtgccaa
    13201 tgacagcagg gacttcacgt agatgcgcgt tggcgtcagc aggcggtcga acagcgtctt
    13261 gccgtcgagc tgggttttgt cgtccgcccc cgcgaccgaa accagtttgc ggatgagcga
    13321 atagccgttc gaatgcgggc cggatgaggc gaggccgatg accacgtcgc cggccgcgat
    13381 cttggtgccg tcgatgatcc ggtcgcgctc gaccacgccc acgcaaaaac cggcgaggtc
    13441 gtaatcgtcg ccgtggtaca tgccgggcat ttccgcggtc tcaccgccga ccagcgcggc
    13501 gccggcgagc tcgcagcctt cggcgatgcc acgaatgacg gcttcggcca cgtcgacgcg
    13561 cagcttgccg gtggcgtagt aatcgaggaa gtagagaggt tcggcaccgg acaccacgat
    13621 gtcgttggcg cacatggcca ccaggtcgat accgatcttg tcgtgccggc ccgtgtcgat
    13681 ggcgaggcgt agcttcgttc ccacgccgtc ggttccagag accagcaccg gccggcgata
    13741 gcggtcgagc ggcacttcga ccagcgcgcc gaagccgccg attcccgcga gaacctcctt
    13801 gcgcatggtc ctcttgacca gcggtttgat gcgttcgacc agttcatcgc ccgcgtcgat
    13861 atcgacgccg gcgtcgcgat aggtgagtcc ggtttcagtc atgaatggag gattctctgc
    13921 gccgggttgc cgacgcggca ggtcgggaaa aaacgtgcga tattgtagcc aatgcgcaca
    13981 ttcgtcgtca gtttcatcgc ggtcgcgttg cttgcattcg cagctgaagc gagggccacg
    14041 cgccaggtgc gcgtgttcga ggccaacgtc tcttcacaaa ccgatccggc ggtgcaggcc
    14101 caggcagcgc tgcg
//
LOCUS       DASQJS010000102        20796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3823484, whole genome shotgun sequence.
ACCESSION   DASQJS010000102 DASQJS010000000
VERSION     DASQJS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20796
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3823484"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..2169)
                     /locus_tag="VEW08_04080"
     CDS             complement(52..2169)
                     /locus_tag="VEW08_04080"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HYJ39944.1"
                     /translation="MSNDLIRRYVTRSLPIVAALSFASPAAYAWDFQNDDGTFSGSWD
                     TTFTYGQAWRIESRDCRLIAIADGGCGRSPNIDDGNLNYDTDMYSRALKVLTELSLNY
                     RGVGAFVRASGLYDDAANTTQRTPLSDKSEDLVVSYTRLLDAFVYAKFDLGSMGAELR
                     LGRQVVSWGESTFIQNGANVINHFDVSALRVPGSELKEGFLPQEMVNFSLQFNDNWSA
                     QAIYITDWNATVPEPAGSYYSSNDFAPTGGSKVVLGFGSFSDQGVDFRPLGGPYITNF
                     QTVNRLPTIKASDDGQYGFNLKYFAPDFNNGTEFGLYFLNYHSRLPLISGMTGTQQGV
                     GNAFGALNAVGAAAQALGAGLAPPAAIATAANLAVARSAAAGGNLSLATATQYATIGA
                     NTQLAHGDVAAQASNIATHEYALTSGYFTEYPEDIKLIALSFNTQLQWGGIALQGEVT
                     YRQDVPLQFDDVELLFAALTPFEQGLATLRGTPLPTTCPEGVPAASTLTHCGQMGSFG
                     LNQKVQGWDRYDTWQVQFTATKTFSNVLKASQMVLVAEAGLTQIPDMPDKLTGGPNDR
                     GLRFNGPGTNVSGNYELRGRHCPTLSAADCLALNLVEPQNRFADETSYGYRVAGRLEY
                     PGLMGPWNVLPRFSWQHDVSGTTPGPGGNFVEGRYGLTLGVAANLLAKWELDMAWTKF
                     GGAGRFNDINDRDFVAATIKFSF"
     gene            complement(2381..4810)
                     /gene="plsB"
                     /locus_tag="VEW08_04085"
     CDS             complement(2381..4810)
                     /gene="plsB"
                     /locus_tag="VEW08_04085"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF003441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HYJ39945.1"
                     /translation="MSWFLRRFFKLWVRAAVQPAEAPPALTAPSVPVCYVLERDSVAD
                     LAVLCNVASKAGLPYPEKRGSSLPVEERRTYFDVARRRRFWDATQTRRPPPYLLALVE
                     ALSADSVRDILLVPTAVYWGRAPQKEGSWWRLLFAENWALTTRVRKFFAVLVNGRNVM
                     VEMGEPISLRSLLDASRVNDQARRVTRILRGILRRQRAIRIGPDLSHRRTIVAQVLRA
                     RAVRAVVASEARDKHGDFRPGLLQARKYAFEIAANYSHAFVQFAEKLLGRVWNRVYDG
                     VKISHAGTLKDVSEGNEVVYVPCHRSHMDYLLMSYIIYHQGYALPHIAAGINLNIPVV
                     GRFLRKGGAFFIRRSFGGNALYTVVFMKYLAAIMARGHSIEYFIEGGRSRTGRLLHPK
                     TGMLSMTVRSFLRDPVRPVVFVPVYFGYERIVEANTYLSELSGAPKKKESWLDLVFSL
                     RVLRERFGTVHVNVGEPIRLNDMLDAQLPAWREQKFDDDTRSPAINALIGDLALNIMR
                     GINSAAAVTPINLLATCLLASPRGALPESALLRQLELYLKLLEVWPYGPRVTVTDANP
                     GEIVLYGESLKIISRVPHKLGDIVKMSDESAQLIAYYRNNVLHLFALPSLVACAFIGN
                     SMLRTEDIQRLAWRIYPYVASELFLRWREDELAKVVDGVLAALAELGVLHPNEDRSVW
                     MRPPPDSPGALQLSMLSQATIQTIERYYLAIALLLKAGSGVISQKALEQRCQLAAQRI
                     NMLYGFNSPEFFDRSLFENFIDLLRERGVLKAGEGGNLEFDEVLVRVAADAQLVLSEQ
                     IRHSILQVTQV"
     gene            4874..5677
                     /locus_tag="VEW08_04090"
     CDS             4874..5677
                     /locus_tag="VEW08_04090"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ39946.1"
                     /translation="MTEIAKLRVILTGASEGIGRALALALAAQGARLALAARDRQRLE
                     TLAQECRALGGEARALPTDVTNEQDCEWLVMETVKAFGGIDVVIHNAGITMWSRFDAL
                     QDLAVFERIMEVNYLAPVRLTALSLPHLKQSKGLLVAVASLAGLTGVPERSGYAASKH
                     AMIGFFDSLRIELRGSGVDVSVVAPDFVVTEIHKRAIGPDGEPLGESPMQQSKIMTAE
                     DCAKRIVRAIGKRERLVLMSARGRLGRWLKLLAPSLIDRIAERAIRQRH"
     gene            complement(5823..6281)
                     /locus_tag="VEW08_04095"
     CDS             complement(5823..6281)
                     /locus_tag="VEW08_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYJ39947.1"
                     /translation="MTNYKFLAPLVLTAALIGQADAAKEDPAVIVAALDTEYQVAVER
                     NDWQTMDRILHPEFTLVLGNGKVYSRAELIESARDKHIEYEKQVEMPGTQTVRVFGND
                     TATVTALLWLKGTRKKDASTFEYKLWFSDTYVRTKDGWRYAFGQASLRLP"
     gene            complement(6281..7183)
                     /locus_tag="VEW08_04100"
     CDS             complement(6281..7183)
                     /locus_tag="VEW08_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYJ39948.1"
                     /translation="MQITNTRAANAPHLALVLGSGGVRSVAALGIADVLTKDGWSPDL
                     VVGCSSGALFGACIAMGMTGEQGLAAATRLWSAELTQQKRWSAYFELLAPRLLGFDAG
                     FALRDAGMIRTRVERAFGDAQFESLPISLRVVATDTATGNPVVLSQGRVSEAVRASMA
                     LPFIFPSVEIDGRRLSDGVISDPLPVAAAGDADAVITLGFRGAMPRRIDRPARMVAQV
                     TTAMINNLQQARVEAARAAGQRMLHIELEIDRRVGLWDTAAMPRIFEIGQRAAIDQLP
                     AIRRMLESPRRSPPDSPYAAVSNL"
     gene            complement(7186..7905)
                     /locus_tag="VEW08_04105"
     CDS             complement(7186..7905)
                     /locus_tag="VEW08_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39949.1"
                     /translation="MRELAQAKRHYSKLPLFEFLRAESIPPRDRLAFYPCMAPFILAF
                     SDLNRFVLRDEASRDPHQLLINEHTYEDDHHWPWYLEDFTKLGFDRTASVSQTLRSYM
                     KDDAQQNRMLGARLAQLVCDTTPTEKLVIVEAIEETGNVLFGLTAKIAARVQEEGGPE
                     LRYLGQFHFSRETGHAMHGHDHRVLEAITLTESERVGCLDLAFRVFDLFTDWTTELLA
                     YATNSLATRTVPHVIHANMKF"
     gene            complement(7984..10665)
                     /locus_tag="VEW08_04110"
     CDS             complement(7984..10665)
                     /locus_tag="VEW08_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYJ39950.1"
                     /translation="MPITATAWKRLSPLLDAALDLAPAERAAWLASLPAEHDDLRESL
                     VELLAKPVETDDFLNRLPEFSGSPVALTLAPGSIVGPYRLLRELGQGGTSSVWLAERV
                     DGSIKRSVALKLPHLGLIDRGIEQRIAREIEILSSLEHPNIARIYDAGIDERGRPYLA
                     LEYVDGVPPDEYCHARRFTPHDKLVLFLNILRAVAFAHARLIVHRDLKPNNILIAGGG
                     EVRLLDFGIARLLQPDTSLRPQQTVVGAAALTPAYAAPEQFTGQSVTVATDVYSLGVI
                     LFELLTGCSPYSPNGRSLGAYEHEVRYVESPLMSRTARPAEAGALRGDLDSIVAKALE
                     KKPQDRYASVEAFAADIERHLAALPIAARPRSFAYVARKFMRRNAWPLSIGAGVVLLL
                     AGSLGFALFQWRDAERQSVMAVERLADSRAAEEFTSTVLIENIQPGQTLTFEQLIARS
                     EQIAQDTGSNDLRTRIFATDFLSAWYSANGLYRNAEVILTRTINTLPADEPLVGATLR
                     CRRARLWDELGQREDAVAVLDDLIARAEADDAIASRCLLDRASVAASKADAHGALAFA
                     LEAQRRYDLTGVESVFDRANILTSIGGAYGIIGEFGASNERYSEALRRFEAAGRGNSR
                     AAANVRDDWATIWMNSGNPLRALEEIDRGWEIMRDFAPAAQDTDKRLSRRARILAQLG
                     NFDAALAQFRQAAKLAAPRGNLANLVTIRIGEADVHVQTGRLEQAGLDLDAAAATLRQ
                     ARLSPTSLISVRYELTRAEWLAARGETAAARDALTRVLGLYESQNCCRATRSLVLAER
                     AMLELDDLDNADADAARARELAPLPEADSFSRFTGSAWYVTALAFEARGKFREARDAF
                     ATAAVQFAGALGDTHADTARARAGIARVSAAATKNDN"
     gene            complement(10709..11251)
                     /locus_tag="VEW08_04115"
     CDS             complement(10709..11251)
                     /locus_tag="VEW08_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HYJ39951.1"
                     /translation="MSLLIQRAHAGDEAARERLFAVAYEDLRGQARKQLRDGGRNTLL
                     NTTVLLHESFLRFQRAGKLAGAERGHFFAYAARVMRSVIVDFARQRQAERRGGDAVHV
                     PLDAQFAEDLRAGEDEVIQINDAIEALQAEDPRLVQVVEMRYFAGMTEQEIATVLGVT
                     DRTVRRDWEKARLLLAAMMR"
     gene            complement(11351..11872)
                     /locus_tag="VEW08_04120"
     CDS             complement(11351..11872)
                     /locus_tag="VEW08_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ39952.1"
                     /translation="MTDRYVVFSHGKDAGPWGVKITALAGNARSEGYEVESVDYRGID
                     DPRERVNKLVEFCKELKGDLVLCGSSLGGYVSVAAASLLHARGVFLMAPALTMPGLPP
                     LREKLLDCPTTIVHGWKDDVVPVEQSMEFARRHGCALHILNSDHRLQDEVRFLKYLFE
                     YFLISLDLPQDVS"
     gene            complement(11881..12243)
                     /locus_tag="VEW08_04125"
     CDS             complement(11881..12243)
                     /locus_tag="VEW08_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HYJ39953.1"
                     /translation="MTNGTPANTPEARAAAIRRAVCAVKRGTFASYGEIALRAGLPGR
                     ARLVGQVLRESGDEKRLPWHRVTGAGGRIAFPAGSVNARKQLRLLAGEGVKTRGNRAL
                     AARKPAARKSLDALLWKI"
     gene            12292..12837
                     /locus_tag="VEW08_04130"
     CDS             12292..12837
                     /locus_tag="VEW08_04130"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HYJ39954.1"
                     /translation="MVRVATDTQPARPQMDWLTMNTTTTAGPTLRAWARARSSAFGRW
                     LFARTVCKRAPYFGTIRPKFLDLEPKLCRVTMKKRRAVENHIGTVHALAMGNLCELAA
                     GLCTEVTIPVGMRWIPRGMTIEYLAKAETDVVATARLDKTEWAGPENIGVPVTVTDTN
                     GKEVVRAVISMYVSPKRESQG"
     gene            complement(13107..13838)
                     /locus_tag="VEW08_04135"
     CDS             complement(13107..13838)
                     /locus_tag="VEW08_04135"
                     /inference="COORDINATES: protein motif:HMM:NF021385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HYJ39955.1"
                     /translation="MLTVKTTTDGLADRVNALDWSALHASLDARGSARIERLLSETEC
                     RALATLYAKDDVFRSRIVMKRHGFGRGEYRYFAYPLPRLVSELREAFYPWIAPIGDRW
                     NERLGRETRFPARHADFLARCHAAGQQRPTPLLLRYGPGDYNCLHQDLYGEHVFPLQL
                     AVLLSEPGQDFSGGEFVLTEQRPRRQTRVEVCDLRRGDAVVFAVHHRPVQGARGDSRV
                     SMRHGVSELRSGERHTLGVIFHDAA"
     gene            13947..14957
                     /locus_tag="VEW08_04140"
     CDS             13947..14957
                     /locus_tag="VEW08_04140"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HYJ39956.1"
                     /translation="MMSGPSPAQRALLARFAKSSLPRALWQLANTLPTFLALWVAMAW
                     TREAGWSYWWTLLLALPTAALYVRLFILQHDCSHGSFFASSRANRWVGASLGLLTLFP
                     FAYWKKTHGVHHATSGNLDQRVLGDIRTLTVDEYRAQSSWMRFCYRFYRSMPVMLGIG
                     PMYQFVAKHRLPLGMPWSWKKEWRSIALNNFSLLAAGLIFGALLDWRTLLLVHLPVVG
                     IAGAFGVWLFYVQHTFEPSYWVRRNHWDPLAAAVEGSSFYDLPAVLRWITANIGYHHI
                     HHLAPRIPNYHLRAAHMAVPELRATVRMGLRESLACAGMKLWDERLGRMVGFPRPAAR
                     RT"
     gene            15048..15437
                     /locus_tag="VEW08_04145"
     CDS             15048..15437
                     /locus_tag="VEW08_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ39957.1"
                     /translation="MTATTTPPRNSTHILIVDDSREAGKSLAQMLTDNGCDEIRTVRS
                     AARALAVAATFQPAIAFLGIPTPDGLEGNLAALLQKNSREKSIRLIGLTDRPEPPGRE
                     EARASGVERFISKPVTQVDLEKALRKS"
     gene            complement(15467..17194)
                     /locus_tag="VEW08_04150"
     CDS             complement(15467..17194)
                     /locus_tag="VEW08_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39958.1"
                     /translation="MAEPFDSQVQTAEQHGSIRVIARAATSIAAFVGRTLKGPVNEPV
                     PVRSFSDYSRVFGGLWQPSTLSYAVDQFFENGGRCAVIVRVVNGARPPTLSLPCSSGK
                     LILRGVGAGSREFLRASVDYDGIDVKETDRFNLVVQRVRTAGSEQIEDQEIYRRLTVT
                     PGADRFVADALLESRLIRVQGGVPGARPSRTPAAASGASIGYTPSNPDGDDGGPITDY
                     DIIGSAQAGTGIFALHSAPEINLLCIPPLTRDTDVGLSTLLVAGRFCRDRRAMLIVDP
                     PSSWASSAAALDGMRHWPFRSEDATMFFPRVVAFDRLRGRFELFAPCGVAAGIIARCD
                     ESTPVWSAADGDAPILRHGMRPAWNVNNADRVRLANAGINLLQAVRIAPKFATSPRTL
                     ASGSAAVSDWRYLSARRLALFVLASVERGTRWMLFERNAQATWTMARAQVEAFLDSLY
                     AEGAFAGRSGEQSYFVVCDERINTEDSMRAGKINLAFGFAATKPGEYHAFIVTHQPSG
                     SRAKVISVNRFANYSPSVDLEIESSILRGLVPLAPVVPPLGSVTESTGAEAPAISVAD
                     TPLAPYLEA"
     gene            complement(17262..17975)
                     /locus_tag="VEW08_04155"
     CDS             complement(17262..17975)
                     /locus_tag="VEW08_04155"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYJ39959.1"
                     /translation="MVIPLHDDNPTSTTPYVTVGIMVACVLAYLFQHLLLSDAGTREA
                     AYAFGAVPAVLTGEKYLPADVAVIPPWATILTSMFMHGGFWHLAGNMLYLWIFGNNLE
                     DAMGHVRYFLFYVLCGVGAVYTFVLLNPASEMPMMGASGAISGVLGGYLLLFPRARVL
                     LGLPLGFIIVGFGRFPAIWVLAAWFVMQLVLGGLSAIQSTGESQGGIAFSAHIGGFIA
                     GLVLVTFFKRRNVPLWRRY"
     gene            complement(18014..19246)
                     /locus_tag="VEW08_04160"
     CDS             complement(18014..19246)
                     /locus_tag="VEW08_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HYJ39960.1"
                     /translation="MKTQSALRALIVTALGVALVSGCSLFGDGPKWGAKRDAEKAAKA
                     LPSLPEPVPTHRFEIDPTVDIVGVVQKTRATKEDTLTDIARRFNIGYEEIVRANPGVD
                     PWLPGENREIVIPSQFILPNAPREGIVINAAAMRLFYYPKVKKGETPVVHTYPIGIGK
                     VGWKTPEGTTTVVRRTKDPVWRPTASIIKEHREERGEELERVVKAGPDNPLGRFAFYL
                     GWPTYMIHGTNKPAGVGLRSSHGCIRLYPEDIAQLFELAPIGTQVRVVNQPFLFGWHE
                     GALHIQAFDVLEDDPRDWKKAQAKLLNKAMAERIQNELKARKEKIDWEAVSRLTHEPR
                     GIPVSVSGGPDQLEHIVASATIVQNKVAPGATWDGISDLPVDEATFQELVSDRESSVE
                     SAAAAAAPAAPTTKPVNN"
     gene            complement(19294..19791)
                     /locus_tag="VEW08_04165"
     CDS             complement(19294..19791)
                     /locus_tag="VEW08_04165"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HYJ39961.1"
                     /translation="MAIARTLRHLFATRAGTRRRFTDVVDASIESAIRAAETRSSGEI
                     RFVIETALDLRELWGAVTPRARALQVFSALGVWDTELRNGVLIYVLAADRDVEIVADR
                     GAATRISPIEWESACKLIEDHFRAGRFREGSVAGVEEVGRLLEREFPVRPGDRDELPN
                     QASLL"
     gene            complement(19799..20683)
                     /locus_tag="VEW08_04170"
     CDS             complement(19799..20683)
                     /locus_tag="VEW08_04170"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HYJ39962.1"
                     /translation="MRAALLTLALLLAGQAPAVRAQIPVPKFEALVVDLTGTLTAAQQ
                     SALDEKLTAFQARKGSQVALLILPTTQPEDIAQFGVRLAEAWKVGRKNVDDGAILIVA
                     KDDRALRIEVQYGLEGSLTDAISSRIINDTIVPLFKQGDFFGGINAGLDQILKVVDGE
                     PLPEPDQAWKPGGSDGAPLPFLLFAGFAALSFLRNFIGRAPLALIAGLGGGALTWWLT
                     SRIPLSAGVGVGLFLLSLMMGGRGGFGGHRTGRVFRDIGRGGGFGGWGGGMGGGGGGG
                     FGGGMGGRGGGGGASGRW"
BASE COUNT         3730 a         6726 c         6767 g         3573 t
ORIGIN      
        1 cagcggcaat caggaaaagt tttttcagca tggagattcc cctcgtcgcg atcagaacga
       61 gaacttgatg gtggccgcga cgaagtcgcg gtcattgatg tcgttgaagc gcccggcgcc
      121 gccgaacttg gtccaagcca tgtccagctc ccacttcgcc agcaggttgg ccgcgacacc
      181 gagcgtgagg ccgtaccggc cctcgacgaa gttgccgccc gggccgggcg tggtgccgga
      241 gacatcgtgc tgccagctga agcgcggaag gacgttccat ggccccatga ggccggggta
      301 ttccaggcgg cccgcgactc gataaccata ggacgtctca tcggcgaaac ggttctgcgg
      361 ctcgacgaga ttcaacgcga gacagtccgc cgcagacagc gtcgggcagt gccggccgcg
      421 cagctcgtag ttgccgctga cattcgtgcc cgggccattg aagcgcaggc cgcgatcgtt
      481 cgggccgccc gtgagcttgt cgggcatgtc cgggatctgc gtcaatccgg cttccgcgac
      541 caggaccatc tgcgaggctt tcagcacgtt ggaaaaggtc ttggtggccg tgaactgcac
      601 ctgccaggtg tcgtaccgat cccagccctg caccttctga ttgagaccga agctgcccat
      661 ctggccgcaa tgcgtgagcg tggaagccgc cggcacccct tccgggcagg tggtgggcag
      721 cggcgtgcca cgcaacgtgg cgaggccctg ctcgaagggt gtgagcgcgg cgaacagcaa
      781 ctcgacgtca tcgaactgca gcggcacatc ctggcgatag gtgacttcac cctgcagggc
      841 gatgccaccc cattgcagct gagtattgaa cgacagggca atgagcttga tgtcttccgg
      901 atactcggtg aagtagccgg acgtgagcgc gtactcgtgc gtggcgatgt tgctggcctg
      961 agcggccacg tccccatgtg cgagctgtgt gttcgcgccg atggtggcgt actgcgtcgc
     1021 cgtcgccagc gagaggttgc cgcccgcggc tgcggagcgc gcgaccgcca ggttggcggc
     1081 cgtggcaatg gccgcgggag gcgcgagccc cgcgcccagc gcctgcgccg cggcaccgac
     1141 ggcattcagc gcgccgaacg cgttgccgac gccctgctgg gtgccagtca tgccggagat
     1201 cagcggcagt cggctgtggt agttcaggaa gtacaggccg aactcggtac cgttgttgaa
     1261 gtcgggtgcg aagtacttga ggttgaatcc gtactgacca tcgtcggagg ccttgatggt
     1321 cggcagacgg ttcacggtct ggaagttggt gatgtacggg ccgccgagcg ggcggaaatc
     1381 gacgccctgg tcggagaacg aaccgaaacc gagcaccacc ttactgccgc ccgtgggcgc
     1441 gaagtcgttg ctgctgtagt acgagccggc gggttccggc accgtcgcat tccagtcggt
     1501 gatgtagata gcctgcgccg accagttgtc attgaactgg aggctgaagt tcaccatttc
     1561 ctgcggcagg aaaccttcct tgagctccga gcccggcacg cgcagcgcgg acacatcgaa
     1621 atggttgatg acgttggcgc cgttctggat gaacgtgctt tcgccccagc tcacgacctg
     1681 ccggccgagg cgcagttcgg cgcccatcga acccaggtcg aatttggcat agacgaaggc
     1741 gtccagcagg cgcgtgtagc tcaccaccag gtcttcggac ttgtcggaaa gcggcgtgcg
     1801 ctgggtggtg ttggcggcgt cgtcatacaa cccggaggcg cgcacgaagg cgccaacgcc
     1861 acgatagttg agcgacagct cggtcagcac cttgagggcg cgcgagtaca tgtccgtgtc
     1921 gtagttgaga ttgccgtcgt cgatgttcgg cgaccggccg caaccgccgt cggctatggc
     1981 gatgagccgg cagtcacgcg attcgatgcg ccacgcctgg ccgtaggtga acgtggtgtc
     2041 ccagctaccg gagaacgtac cgtcgtcgtt ctgaaaatcc caggcatagg ccgcgggcga
     2101 cgcaaagctg agcgccgcga cgatcggaag cgaacgcgtc acgtatcgcc ggatcagatc
     2161 gttactcatt aatttcccct gagaccgcgg aaatgctgcg agtcgcgagg tcgggagcag
     2221 cagtaccgct tggccttatc gtctttagtg ttccgccgcg ctgctgcttc ccctcgcgga
     2281 cgcgtaggcg cggacgtgtt gcgcgcgagt atagcagcgg gttccttagg gaaagctagc
     2341 cggagccccg gcgggtatac cccgggggaa tccctagcac ctagacctgc gtgacttgaa
     2401 ggatgctgtg cctgatctgt tcggacagca cgagctgcgc atcggcggcg acgcggacca
     2461 gcacttcgtc gaattccagg ttgccaccct caccggcctt caggacgccg cgttcgcgca
     2521 gcaggtcgat gaagttctcg aacagggagc gatcgaagaa ttcgggcgaa ttgaatccgt
     2581 acagcatgtt tatccgttgg gccgccagct ggcaccgctg ctcgagcgcc ttctgactga
     2641 tgacgccgct gcccgccttg agcaggagcg cgatcgcgag gtagtaacgc tcgatggtct
     2701 ggatggttgc ctgcgacagc atggaaagtt gcagcgcgcc cggcgaatcc ggcggcggcc
     2761 gcatccagac gctgcgatct tcgttagggt gcaacacgcc cagctccgcc agcgcagcaa
     2821 gaaccccgtc gacgaccttt gccagttcat cctcgcgcca gcgcaggaaa agctcggagg
     2881 cgacatacgg ataaatacgc caggccaggc gctgtatgtc ctcggtacgc aacatggagt
     2941 tgccgatgaa cgcacaggcg accagcgatg gcagcgcgaa caggtgaagg acgttgttgc
     3001 ggtagtaggc gatgagctgc gcgctctcgt cgctcatctt cacgatgtcg ccgagcttgt
     3061 gcggcacgcg ggagatgatc ttgagcgact cgccgtagag cacgatttcg ccgggatttg
     3121 cgtcggttac cgttacccgg ggtccgtatg gccatacctc caggagcttc agatacaact
     3181 cgagctggcg aagcaacgca ctctcgggca acgcgccgcg cggcgacgcg agcaggcagg
     3241 tcgccagcag attgatgggc gtgaccgccg cggcggagtt gatgcccctc atgatgttca
     3301 gcgccaggtc accgatgagg gcattgatgg cgggcgaacg cgtgtcgtcg tcgaatttct
     3361 gttcccgcca tgcggggagc tgcgcgtcga gcatgtcgtt cagccggatg ggctcaccca
     3421 cgtttacgtg caccgtaccg aagcgctcgc ggagcacacg cagggagaac accaggtcga
     3481 gccagctctc cttctttttg ggggccccgg acaactcgga taagtacgtg ttggcctcga
     3541 cgattcgctc gtagccgaag tagacgggga cgaacaccac cgggcgcacc gggtcgcgca
     3601 ggaagctgcg caccgtcatc gacaacatgc cggttttcgg gtgcagcagc cgcccggtgc
     3661 gcgaacgccc gccctcgatg aaatattcga tggagtggcc gcgcgccatg atggcggcga
     3721 ggtacttcat gaagaccacg gtgtacagcg cgttgccgcc gaagctgcga cggatgaaga
     3781 acgcgccgcc cttgcgcagg aagcggccca ccaccgggat gttcaggttg atgccggcgg
     3841 cgatgtgcgg cagcgcgtaa ccctggtgat agatgatgta cgacatcagc aggtagtcca
     3901 tgtggctgcg atggcagggc acgtagacca cctcgttgcc ttccgacacg tccttcagcg
     3961 tgccggcgtg gctgatcttc acgccgtcgt acacgcggtt ccatacgcgc ccgagcagct
     4021 tctccgcgaa ctgcacgaaa gcgtgcgagt agttagcggc gatctcgaag gcgtatttgc
     4081 gcgcctgcag caggccgggg cggaaatcgc cgtgtttgtc gcgcgcctcg gatgcgacca
     4141 cggcgcgcac ggcgcgtgca cgcagcacct gcgccacgat cgttcggcga tgggaaagat
     4201 ccggcccgat ccggatggcg cgctgccggc gcaggatgcc gcgcaggatg cgcgtcacgc
     4261 gccgcgcctg gtcgttcacc cgcgatgcgt ccagcagcga gcgcaggctg atcggctcgc
     4321 ccatttccac catgacgttg cggccgttca cgagcaccgc aaagaacttg cgcacgcgag
     4381 tcgtcagcgc ccagttctcg gcaaacagca ggcgccacca cgacccctcc ttttgtggcg
     4441 cgcgccccca gtacaccgcg gtgggcacga gcaatatgtc gcgcacggaa tcggcgctca
     4501 gcgcctcgac cagcgccagc aggtacggcg gcggccgccg cgtttgcgtg gcgtcccaga
     4561 agcggcgacg gcgggcgacg tcgaaatagg tacggcgctc ttccaccggc aggctgctcc
     4621 cgcgcttttc cggataaggc aatcccgcct tgctggccac gttgcacaac acggcgaggt
     4681 cggccaccga atcgcgctcg agcacgtaac agacgggcac gctcggcgcc gtgagcgccg
     4741 gcggcgcctc ggcgggctgc acggcggccc ggacccagag cttgaagaat cgtcgcagga
     4801 accagctcat gcgtttacgc gtgccacccg ccgtatgatg ttaggcgccg gagtgtagcc
     4861 gagagcctga ggcatgaccg aaatcgcaaa attgcgggtg attctgaccg gtgcatccga
     4921 gggcatcggc cgtgcgctgg cgctggcgct cgccgcgcag ggcgcacggc tggcgctcgc
     4981 cgcacgcgac cgccagcgcc tggaaacact ggcccaggaa tgccgtgcgc tcggcggcga
     5041 agcccgcgcc ctgccaaccg acgtcaccaa cgagcaggac tgcgagtggc tggttatgga
     5101 aaccgtgaag gccttcggtg gtattgacgt agttatacac aacgccggca tcacgatgtg
     5161 gagccggttc gacgcgctgc aggacctggc ggtcttcgag cggatcatgg aagtgaacta
     5221 cctggcgccc gtgcgcctca ccgcgctgtc gttgccgcat ctcaagcaat cgaaagggct
     5281 gctggtggcg gttgcgagcc tcgcggggct caccggcgtg cccgaacgct cagggtacgc
     5341 cgccagcaag cacgcgatga tcggcttctt cgattcgctg cgtatcgagc tgcgcggcag
     5401 cggggtcgac gtgagcgtgg tcgcgccgga tttcgtggtg acggaaatcc acaagcgcgc
     5461 catcgggccg gatggtgaac cgctcggcga gtcgccgatg cagcagtcaa agatcatgac
     5521 cgccgaggac tgtgcgaagc gcatcgtgcg cgcgatcggc aagcgtgagc ggctcgtgct
     5581 gatgtcggcg cgcggccgtc tcggccgctg gctcaaacta ctcgcgccgt cgctgatcga
     5641 ccgcattgcc gagcgggcga tccgccagcg ccattgaccc gtcggtcaag ccaggaactg
     5701 gggctgcccc tttttcatga actgcgcccc agcaagcgca cccgcacagg ggctgagagg
     5761 ggacccgact ccggtgcgag ggcagtctcg ctgaggcgca gtacgtctgg cggcgtcgtc
     5821 agctacggca aacgcagcga tgcctgcccg aacgcgtagc gccagccgtc tttcgtccgc
     5881 acgtacgtgt cgctgaacca gagcttgtat tcgaacgtgc tcgcgtcttt cttgcgcgtg
     5941 cccttcagcc acagcagcgc ggtgaccgtg gcggtgtcat tgccgaacac gcgcacggtc
     6001 tgcgtaccgg gcatctccac ctgcttctcg tattcgatgt gtttgtcgcg cgcggattcg
     6061 atgagctcgg cgcgtgaata gaccttgccg ttgccgagca ccagcgtgaa ttccggatgc
     6121 aggatccggt ccatggtctg ccagtcgttg cgctccaccg ccacctggta ctcggtgtcg
     6181 agcgcagcca cgatgaccgc gggatcttct ttcgcggcgt cggcctgccc gatgagcgcg
     6241 gcggtcagca ccagtggagc gaggaatttg tagttagtca tcataggttg ctcactgcgg
     6301 cgtacggaga atcgggcggc gaccggcgcg gcgattcgag catccgccgg attgccggca
     6361 actgatcaat cgcggcgcgc tggccaattt cgaaaatgcg cggcatggcg gcggtatccc
     6421 acagcccgac gcggcgatca atctcgagtt cgatgtgcag catgcgctgg cccgcggcgc
     6481 gcgccgcttc gactcgtgcc tgctgaagat tgttgatcat cgcggtggtc acctgggcaa
     6541 ccatgcgggc cggccgatcg atgcgccgcg gcatggcgcc gcgaaagccc agcgtgatga
     6601 cggcatcggc atcacccgcc gccgccacgg gcagcggatc ggagatcact ccatcactca
     6661 gtcgccgccc gtcgatttcc acgctcggga agatgaaggg cagcgccatg ctggctcgta
     6721 cggcttccga cacgcgcccc tggctgagca ccaccggatt tcccgtggcc gtgtccgttg
     6781 caaccacgcg cagcgagatc gggagactct cgaactgcgc atcgccgaat gcacgctcga
     6841 cacgcgtccg gatcatgccc gcatcgcgca gtgcgaaccc tgcgtcaaac cccaatagcc
     6901 gcggcgccag cagctcgaag tacgcgctcc agcgcttctg ctgcgtgagt tccgccgacc
     6961 acagtcgcgt cgcggccgcg agtccctgct caccggtcat gcccattgcg atacaggcgc
     7021 cgaacaaggc gcccgaactg cagcccacga ccaggtccgg tgaccatccg tcttttgtga
     7081 gcacgtcggc tatacccagc gccgccacgc tgcggactcc accgctgccc agaaccaggg
     7141 ccaggtgcgg cgcattggcg gcgcgcgtgt tggtgatttg catgatcaga acttcatgtt
     7201 ggcgtgaatg acgtgcggaa ccgtgcgggt cgccagcgag ttcgtcgcgt acgccagcag
     7261 ttcggtggtc cagtcggtga acaggtcgaa gacgcggaac gcgagatcga ggcagcccac
     7321 gcgttcagac tcggtgagcg tgatggcttc gagaacgcga tggtcatgac cgtgcatggc
     7381 gtggccggtc tcgcggctga aatgaaactg tccgaggtac cgcagctccg gaccgccctc
     7441 ttcctggacg cgcgccgcga ttttcgcggt gaggccgaac agcacattgc cggtttcctc
     7501 gatggcctcg acgatgacga gcttctccgt gggtgtcgtg tcgcacacca gctgcgcgag
     7561 ccgcgcgcca agcatgcggt tctgctgggc gtcatccttc atgtacgaac gcagcgtctg
     7621 ggagacgctc gccgtgcggt cgaagcccag cttggtgaag tcttcgaggt accacggcca
     7681 gtggtggtcg tcctcgtagg tgtgttcgtt gatcaggagc tggtgcgggt ctcgggaagc
     7741 ttcgtcgcgc aacacgaagc ggttgagatc agagaacgcc aggatgaatg gcgccatgca
     7801 ggggtagaac gccagccggt cgcgcggagg aatcgactcg gcgcgcagga attcgaacaa
     7861 cggcagtttc gaataatgtc gcttggcttg cgccagctct ctcatcacag ccttcatcat
     7921 ttctccagga cttggaacgt ctcatggaga acggacgcag gcgggcttga gttcaggaca
     7981 tggtcaattg tcattttttg tcgccgccgc ggacacgcgc gcgatgccgg cgcgggctcg
     8041 cgcggtatcg gcgtgtgtat cgcccagcgc gccggcgaac tgcacggccg ccgttgcgaa
     8101 tgcgtcgcgc gcctcgcgga acttgccccg ggcttcgaag gccagcgcgg tcacatacca
     8161 cgcgctgccc gtgaagcgtg aaaacgaatc cgcctccggc aacggcgcca gttcccgcgc
     8221 gcgcgccgca tccgcatccg cgttgtcgag atcgtcgagc tcgagcatgg cgcgttcggc
     8281 gagcacgagc gaacgggtcg cgcggcaaca gttctgcgat tcatacaacc cgaggaccct
     8341 ggtcagcgca tcgcgcgcgg cggcggtttc gccgcgcgca gcaagccact cggccctcgt
     8401 caactcgtaa cgcacactga tgagactggt cggcgacaac cgggcctgtc gcagtgtcgc
     8461 cgccgccgcg tcgagatcga gccccgcctg ttcgaggcgt ccggtctgca cgtggacgtc
     8521 ggcttcgccg atgcgaatcg tcacgagatt ggccaggttg cctcgcggcg ccgcgagctt
     8581 ggccgcctga cggaactgcg caagcgccgc atcgaaattg ccgagctgcg cgagaatgcg
     8641 cgcgcggcgc gacaatcgct tgtccgtgtc ctgcgcggcc ggcgcgaaat cgcgcatgat
     8701 ttcccagccg cggtcgattt cttcgagggc gcggagcggg ttgccggaat tcatccagat
     8761 cgtcgcccag tcgtcgcgca cgttggcggc ggcccggcta ttgcctcgcc cggccgcttc
     8821 gaatcgacgc agcgcttcgc tgtatcgctc gttcgatgcg ccgaattctc caatgattcc
     8881 gtaggcgccg ccgatcgacg tcaggatatt cgccctgtcg aacaccgatt cgacgccggt
     8941 caggtcgtag cgtcgctgtg cctcgagcgc gaacgcaagc gcaccgtggg cgtcggcttt
     9001 cgacgccgcc actgaagcac gatcgagcag gcagcgcgag gcgatggcgt cgtcggcttc
     9061 tgcgcgggcg atcagatcgt cgagaaccgc aaccgcgtcc tcgcgctgac cgagctcgtc
     9121 ccacaacctc gcacggcgac agcgcagtgt cgcgccgacg agcggctcat ccgcgggcaa
     9181 cgtgttgatg gtgcgggtga ggatcacctc tgcgttgcga tacaacccgt tcgcgctgta
     9241 ccaggccgac aggaaatccg tggcgaagat gcgggtgcgc agatcgttcg agccggtgtc
     9301 ctgcgcaatc tgctcgctgc gtgcgatcag ctgttcgaac gtcaacgtct gtccgggctg
     9361 gatattctcg atgagcaccg ttgaggtgaa ttcctccgca gcgcgcgaat cggcgagccg
     9421 ttcgacggcc atgacgctct gccgctcggc atctctccat tggaacaagg cgaagccgag
     9481 cgagcccgcc agcagcaaca ccacgccggc accgatcgac aacggccagg cattgcgccg
     9541 catgaacttg cgcgcgacat aggcaaatga ccgcggccgc gccgcgatcg gtagcgcggc
     9601 gagatgcctt tctatgtcgg cggcgaatgc ctccaccgac gcataacgat cctggggttt
     9661 cttttccagc gccttggcga cgattgaatc gaggtcaccg cgcagcgcac cggcttcggc
     9721 gggacgcgcg gtgcggctca tcagcggcga ctcgacgtag cgcacctcgt gttcataggc
     9781 gccgagcgat cggccgttcg gcgaatacgg gctgcacccg gtgagcagct cgaacaggat
     9841 cacgcccagc gaatacacgt cggtggcaac ggtcaccgat tgcccggtga actgctcggg
     9901 cgcggcataa gcgggcgtga gcgccgccgc accgacgacg gtctgctgcg ggcgaaggct
     9961 ggtgtccggc tgcagcagcc gcgcgatgcc gaagtcgagc aggcgcacct caccgccccc
    10021 ggcaatcagg atgttgttgg gcttgaggtc gcgatggacg atgagccgcg catgcgcgaa
    10081 cgccaccgcg cgcaggatgt tgagaaacag cacgagcttg tcgtgcgggg tgaacctgcg
    10141 ggcgtggcag tattcgtcgg gaggcacacc gtccacgtat tcgagcgcga gatagggccg
    10201 gccgcgctcg tcgatgcccg cgtcatagat gcgcgcgatg ttcgggtgct cgagtgaaga
    10261 gaggatttcg atttcgcgcg caatacgctg ctctatgccg cggtcgatga gcccgagatg
    10321 cggcagcttg agcgccacgc tgcgcttgat ggagccgtcg acgcgctccg ccagccacac
    10381 tgacgacgta ccgccctgtc ccaactcgcg caacagccga tagggcccga cgatggagcc
    10441 tggcgcgagc gtgagcgcca cgggcgaacc actgaactcc ggcaggcgat tcaggaaatc
    10501 gtcggtctcc accggttttg ccagcagctc gacgagcgat tcgcgcagat cgtcatgttc
    10561 tgccggcagg ctcgcaagcc aggccgcccg ctctgcgggc gcgaggtcga gcgctgcgtc
    10621 gagcagcggg ctcagtcgtt tccaggcggt ggcggtgatt ggcatcgttc aagaccaacg
    10681 gacggactga cgcgtccaat ccggacattc aacgcatcat ggccgccaac aataaccgag
    10741 ccttctccca gtcacgccgc acggtgcgat cggtaacgcc cagtacagtg gcgatttcct
    10801 gttcggtcat gccggcgaaa taacgcatct ccaccacctg caccagccgc gggtcttcgg
    10861 cctgcagggc ttcgatggcg tcgttgatct ggatgacctc gtcttccccg gcgcgcaggt
    10921 cttcggcgaa ctgggcgtcg agcggcacat gcaccgcgtc accgccacgg cgttcggcct
    10981 gacgctggcg ggcgaaatcg acgatgaccg aacgcatgac gcgcgctgcg taggcgaaga
    11041 agtggccccg ctcggcaccg gcgagcttgc cggcacgctg gaagcgcaga aagctttcgt
    11101 gcagcagtac ggtggtgttc agcagcgtgt ttctgccgcc atcgcgcagc tgtttgcgcg
    11161 cctggccgcg caggtcttcg tacgccactg cgaacagccg ctcacgtgcg gcctcgtcgc
    11221 ccgcgtgagc gcgctggatc aggagtgaca actcatccat gtgatgacgg tgccgggccg
    11281 ggaaaccggg aaaactcggc cggcgcccgt agtacgtgag ttttcccggt ttcccggccc
    11341 ggcaccatat ttacgacacg tcctgaggaa ggtcgagcga gatcaggaaa tattcgaaca
    11401 gatacttgag gaatcgcact tcgtcctgca gccggtggtc gctgttgagg atgtgcaatg
    11461 cgcagccgtg gcggcgcgcg aattccatgc tctgctccac gggcaccacg tcgtctttcc
    11521 agccgtgcac gatggtggtg gggcaatcga ggagcttttc gcgcagcggc ggcaggcccg
    11581 gcatcgtcag ggccggcgcc atcaggaaca cgccgcgcgc gtgcagcagc gaggccgccg
    11641 ccaccgacac atatccgccg agactggagc cgcacagcac gaggtcgccc ttgagctcct
    11701 tgcagaactc gaccagcttg ttgacgcgct cacgcggatc gtcgatgccg cggtagtcca
    11761 ccgactccac ttcgtaacct tcgctgcgcg cattgccggc gagagcggtg atcttcacac
    11821 cccagggccc ggcgtccttg ccatgagaga aaacgacgta gcgatcggtc atggggatcc
    11881 tcagattttc cagagcagtg catcgagact cttgcgggca gccggcttgc gggccgcgag
    11941 cgcgcgattg ccgcgggtct tcacaccttc gcctgcgagc aggcgcaatt gtttgcgcgc
    12001 gttgacggag cccgcgggaa aggcaatgcg gcctcccgcg ccggtcacac gatgccacgg
    12061 caaacgcttt tcatcgccgg actcacgcag tacctggccc accagccgtg ctcgcccggg
    12121 caatccggcg cgaagcgcga tctcgccata actggcgaag gtgccgcgct tcacggcgca
    12181 cactgcccgg cggattgcgg cggcgcgtgc ttctggcgta tttgctggag tgccattcgt
    12241 catgaattca actagagtcc aagccccgcg gcgacaagat tcgatcacgc gatggtccga
    12301 gtagcaaccg acacacaacc cgcccgtccg cagatggact ggttgaccat gaacaccacc
    12361 accaccgcag gccccacgct tcgagcctgg gcacgcgcgc ggagttccgc attcggccgg
    12421 tggttgtttg cgcgcacggt gtgcaagcgt gcgccctact tcggcaccat ccgcccgaaa
    12481 ttcctcgacc tcgaaccgaa actctgtcgc gtcacgatga agaaacgccg tgccgtcgaa
    12541 aatcacatcg gcaccgtgca tgcgctggcc atgggaaatc tctgcgagct cgccgccggc
    12601 ctgtgcaccg aagtcaccat tcccgtcggt atgcgctgga tcccgcgcgg catgaccatc
    12661 gaatacctgg cgaaggcgga aacggacgtc gtcgccaccg cgcgtctcga caagaccgag
    12721 tgggccggtc ccgagaacat cggcgtgccg gtaactgtca ccgacaccaa cggcaaggaa
    12781 gtcgtgcgcg cggtcatcag catgtacgtc tccccgaagc gcgagtccca gggctgacaa
    12841 caccacgcac ctggaccctg gtcggcgccg atggcgcggc gttcgccagc ccggttccgg
    12901 gaaccttcgg tgggcatcgc aagacgaaaa tctatggccg cctcgactgt cgcgccgcac
    12961 tgcgggccat cgcgcgcggc ggatacgtga aggaacgtgt gttctttgtg gatgaggcag
    13021 cagccgtgtc cgccggcttc agaccctgcg cggtgtgcat gccggaaagt tacgcgcgct
    13081 ggaaggtcag tcgctgacgc gtaacctcag gcggcgtcat gaaagatgac acctaacgtg
    13141 tgtcgctcac cggagcgcag ctcactgacg ccgtgccgca tgctcacgcg ggagtcaccg
    13201 cgcgcaccct gcaccgggcg gtggtgaacg gcgaaaacaa ccgcgtcgcc gcgccgcagg
    13261 tcacaaacct cgacgcgtgt ctgtcggcgt ggccgctgtt cggtcagtac aaactcgccg
    13321 cctgaaaagt cctgcccggg ctccgacagc aacaccgcga gctgcagcgg gaaaacgtgt
    13381 tcgccgtaca ggtcctgatg caggcagttg tagtcgccgg ggccgtaacg caacagcagt
    13441 ggcgtcggtc tttgctgtcc cgctgcgtgg catcgcgcca ggaaatccgc gtgccttgcg
    13501 ggaaatcgcg tttcccggcc caggcgctcg ttccagcgat cgccgattgg tgcaatccac
    13561 ggataaaagg cttcacgcag ctcgctgacg agacgtggca gtggatatgc aaagtagcga
    13621 tattccccgc ggccgaagcc atgacgcttc atgacgatcc ggctgcggaa gacatcatcc
    13681 ttcgcataca acgtggccag cgcgcgacac tcggtctcgc tcagaagtcg ctcgatgcgg
    13741 gcgctgccac gcgcatccag cgatgcatgc agcgccgacc aatcgagcgc attcacgcga
    13801 tccgccaacc catccgtagt cgttttgacg gtcaacacga ggccatttcc gatttcgaag
    13861 ctgttcagat cagtttggtc cgcaaccgca caaatggcat tccgaaactt gctgaacttc
    13921 gcggcgcgaa aggcgcagac tcggccatga tgtccggccc ctctcctgcg caacgcgcgt
    13981 tgctcgcgcg cttcgcaaaa tcttccctgc cacgcgcgct ctggcaactc gcgaacactc
    14041 tgccgacttt cctggctctc tgggttgcca tggcctggac ccgcgaggcc ggctggagct
    14101 actggtggac cctgctgctg gctctgccga cagccgcgct gtacgtgcgg ctgttcatcc
    14161 tgcaacacga ctgcagccat ggttcgtttt tcgcgagctc acgcgcgaac cgatgggtgg
    14221 gagccagcct cggcctgctc acacttttcc cattcgctta ttggaagaag acgcacgggg
    14281 tacatcacgc cacctccggc aatctcgacc agcgcgtgct cggcgacatt cgcacgctga
    14341 cggtggacga ataccgcgcg cagtcatcct ggatgcgctt ctgctaccgc ttctaccgaa
    14401 gcatgccggt gatgctcggc atcggcccca tgtaccagtt cgtggcgaag caccggctgc
    14461 cgctgggcat gccatggagc tggaaaaagg aatggcgcag cattgcgctc aacaatttct
    14521 cgttgctggc ggcgggcctc atcttcggcg cgctgctcga ctggcgcacg ctgctgctcg
    14581 tccacctgcc cgtggtggga attgccggcg cgttcggcgt gtggctgttc tacgtgcagc
    14641 acactttcga acccagctat tgggtccggc gcaaccactg ggatccgctg gcggccgctg
    14701 tcgaaggcag ctcgttctac gatctgccag ccgtgctgcg ctggattacc gccaacatcg
    14761 gttatcacca catccatcac ctggcgcccc gtatccctaa ctaccatctg cgcgccgcac
    14821 acatggcggt gccggaattg cgggcgacgg tgcgcatggg gctgcgcgag agcctggcgt
    14881 gtgctggcat gaagctttgg gacgaacgac taggccgaat ggtcggattt ccccgcccgg
    14941 ccgcccgccg cacctgaaat gcgccggaat cgagaatagg cgggcaggaa actcaggacc
    15001 tgccattctg ctgacccgcg catccggcgc cctggagtac acgcctgatg accgccacca
    15061 cgacccctcc ccgcaacagc acgcacatcc tcatcgtcga cgacagccgt gaggcgggaa
    15121 aatccctcgc gcagatgctg accgacaacg gctgcgacga aatccgcacc gtgcgcagcg
    15181 ccgcgcgtgc gctggcggtc gccgctacct ttcagccagc catcgctttc ctgggaatcc
    15241 cgacccccga cggcctggaa ggaaaccttg ccgcgctgct gcagaagaat tcccgcgaga
    15301 agagtattcg tctgatcggc ctcactgatc gtccggagcc gccgggccgt gaagaggcgc
    15361 gcgcttcggg tgtcgagcga ttcatcagca agccggtgac gcaggtggat ctcgaaaagg
    15421 ccttgaggaa atcgtaggcc atgtgactgg ccagcggcct cgcgacttac gcttcgaggt
    15481 agggcgcgag cggcgtatcc gcgacggaga tggccggcgc ctccgccccg gtggattcgg
    15541 tgactgaccc cagaggcggg acaaccggcg ccagcgggac cagcccgcgc agaatgctcg
    15601 attcgatctc gaggtcgacg ctgggcgaat aattcgcgaa gcggttcacg ctgatcacct
    15661 tcgcgcgcga tccgctcggc tgatgcgtga cgatgaacgc gtggtattcg cccggcttcg
    15721 tggctgcgaa accgaaggcg agattgatct tgccggcgcg catgctgtct tccgtgttga
    15781 tgcgttcgtc gcacaccacg aagtaactct gctcgccgct gcggcccgcg aacgccccct
    15841 cggcatacag ggaatcgaga aaggcctcga cctgggcgcg tgccatggtc caggtggcct
    15901 gcgcattgcg ttcgaacagc atccatcgcg tgccgcgctc gacggaagcc agcacgaaca
    15961 aggccagccg gcgcgcggag agatatcgcc agtcgctcac cgcggccgag ccgctcgcga
    16021 gggtgcgcgg actggtcgcg aactttggcg cgatgcgcac cgcctgcaga aggttgatgc
    16081 cggcattcgc gaggcgaacg cgatcggcgt tgttcacgtt ccacgccgga cgcatgccgt
    16141 gacgcaggat gggcgcatca ccatcggccg ctgaccacac cggggtgctc tcatcgcagc
    16201 gcgcgatgat gcccgccgcc acgccgcagg gcgcgaacag ctcgaatcgg ccgcgcaggc
    16261 ggtcgaaagc cacgacccgt ggaaaaaaca tggtcgcgtc ttcgctgcgg aacggccagt
    16321 gacgcatgcc gtcgagcgcg gcggcgctgg atgcccacga cgacggcgga tccacgatca
    16381 gcattgcgcg ccgatcgcgg cagaaccgcc cggccaccag cagtgtggac aggcccacgt
    16441 cggtgtcgcg cgtgagcggc gggatgcaca gcaggtttat ctccggcgcc gagtgcaacg
    16501 cgaagatgcc ggtgcccgcc tgggcggaac cgatgatgtc gtagtcggta atcgggccgc
    16561 cgtcgtcgcc gtcgggattg gatggcgtat agccgatgga cgcgcccgag gccgccgccg
    16621 gagttcgact cggccgcgcg ccgggaacgc cgccctgcac ccggatcagg cgtgactcga
    16681 gcagtgcatc ggccacgaaa cggtccgcgc ccggggtgac cgtcagccgc cggtagattt
    16741 cctggtcttc gatctgctcg gagccggcgg tgcgcactcg ctgcacgacg aggttgaagc
    16801 ggtcggtctc cttcacatcg atgccgtcgt agtcgacgga cgcacgcagg aattcacgtg
    16861 atccggcgcc gactccgcga aggatgagtt tgccggacga acacggcagc gacagcgtcg
    16921 gcgggcgcgc tccattgacc acgcggacga tgacggcgca gcggccgccg ttctcgaaga
    16981 actgatcgac cgcgtaactc agcgtggagg gttgccagag gccaccgaag acgcgcgagt
    17041 agtcggagaa actgcggacc gggacaggtt cattgaccgg ccccttcagg gtgcggccga
    17101 cgaaggccgc gatgctggtc gcggctcgcg caatgacacg tatagagccg tgttgttcgg
    17161 cggtttggac ttgtgagtca aacggctcgg ccaatcaact ccccaaaaca ttactacccg
    17221 acatctcggg cctctcaacg tgcgcggaat gtagcagcgt ctcagtagcg ccgccacagc
    17281 ggaacgttgc gtcgtttgaa aaatgtgacg agcacgagtc ccgcgatgaa tccgccgatg
    17341 tgcgcgctga aggcaatgcc accctgggat tcgccggtgg actgaatcgc cgagaggcct
    17401 cccagcacga gttgcatgac gaaccacgcc gccagcaccc agatagccgg gaatcgtccg
    17461 aatccaacga tgatgaagcc aagcggcaac cctaacaaca cgcgggcgcg gggaaacagc
    17521 agcaggtaac cgccgagcac accggagatg gcgccgctgg cgcccatcat gggcatctcc
    17581 gaagcgggat tgagcagcac gaaggtgtaa acggcgccga caccgcacag gacataaaag
    17641 aggaagtagc gtacgtggcc catggcgtct tcgaggttgt tcccgaaaat ccacaggtaa
    17701 agcatgttgc cggcgaggtg ccagaagccg ccgtgcatga acatggacgt gaggatcgtg
    17761 gcccagggcg gaatcacggc gacatccgcg ggcaggtatt tttcgccggt cagtaccgct
    17821 gggactgcgc cgaaggcata cgctgcctcc cgcgtgccgg catccgacag cagcaggtgc
    17881 tggaaaaggt acgccagcac gcaggcaacc atgatcccga cggtgacgta cggcgtcgta
    17941 ctcgtcgggt tgtcgtcatg cagcggaatg accacgaatc agcgtgaccg tgccagcacg
    18001 atgccggcag ccgtcaatta ttgacgggct tcgtggtcgg cgcggcgggt gcggcagcgg
    18061 cggcggcgga ttccaccgag gactcgcgat ccgacacgag ctcctggaag gttgcctcgt
    18121 ccaccggcag gtcggagatg ccgtcccagg ttgcgcccgg cgcgaccttg ttctgcacga
    18181 tggtcgcgga cgcaacgatg tgttcgagtt ggtccggacc gcctgacacg gacacgggaa
    18241 tgccacgcgg ctcatgcgtg aggcgcgaca ccgcctccca gtcgattttt tccttccggg
    18301 ctttgagctc gttctggatg cgctcggcca tcgccttgtt gagcagcttg gcctgggcct
    18361 ttttccagtc acgcgggtcg tcttcgagca cgtcgaacgc ctggatgtgc aatgcgcctt
    18421 cgtgccagcc aaacaggaat ggctggttga cgacgcgcac ctgcgtaccg atgggcgcca
    18481 actcgaacaa ctgcgcgatg tcttccggat acaggcggat gcagccgtgg ctggatcgca
    18541 agcccacacc cgcgggcttg ttggtgccgt ggatcatgta agtgggccac ccgagataga
    18601 acgcgaagcg ccccagcgga ttgtccggcc cggccttcac cacgcgctcg agttcctcgc
    18661 cgcgctcttc gcggtgctct ttgatgatgg acgcggtcgg ccgccagacg ggatccttcg
    18721 tgcgccgaac gacggtcgta gtgccctcgg gagttttcca gcccaccttg ccgataccga
    18781 tgggataggt gtgcaccacc ggcgtctcgc ccttcttcac cttcgggtaa tagaagagcc
    18841 gcatcgcggc ggcgttgatg acgatgccct cgcgcggcgc gttgggcagg atgaactgcg
    18901 acgggatgac gatttcgcga ttttctcccg gcagccaggg atcgacgcca ggattggcgc
    18961 gcacgatctc ttcgtagccg atgttgaacc gccgcgcgat gtcagtgagc gtgtcttcct
    19021 tggtggcgcg cgttttttgc accactccga cgatgtcgac ggtcgggtcg atctcgaaac
    19081 gatgcgtggg cacgggctcg ggaagcgacg gcagcgcctt cgccgccttc tcggcatcac
    19141 gtttggcgcc ccacttcgga ccatcaccga acagcgagca gccggagacc agcgccacac
    19201 caagcgccgt gacgatgaga gcccgcagcg cggattgagt tttcatgttc aaagtatgac
    19261 accgcataaa tgattcaggc tgtcggcatt ctgctacagc agcgaggcct ggttcggaag
    19321 ctcgtcgcgg tcacccgggc ggaccggaaa ttcccgctct aacaatcggc ccacctcttc
    19381 gacaccggca accgagcctt ccctgaaacg gcctgcacga aagtggtcct cgatgagctt
    19441 gcacgcggat tcccactcga taggggaaat cctggtggcc gccccgcggt cggccacgat
    19501 ctcgacgtct cgatcggccg ccaatacata gatgagtacg ccgttgcgaa gttctgtgtc
    19561 ccagacacca agcgccgaga aaacctgcag ggcgcgcgcc ctcggcgtga ctgcgcccca
    19621 gagctcgcgc aggtcgagtg cggtttcgat cacgaagcgg atttcgccgg aactgcgcgt
    19681 ttccgccgcc cggatggcgg attcgatgct ggcatcgacc acgtcggtga aacggcgacg
    19741 cgtgcccgcg cgcgtggcaa acaggtggcg aagcgttcgc gcgatggcca tgtactaact
    19801 accagcggcc ggacgcaccg ccgcctccgc cgcgaccgcc catgccgccg ccgaatccgc
    19861 ccccaccgcc acctcccatc cctccgcccc acccgccgaa gccgcccccg cggccgatat
    19921 cgcgaaacac gcggccagtg cgatggccgc cgaatccgcc gcgaccaccc atcatcagcg
    19981 acagtaagaa gagtccgacg cccacgccgg cagacagagg aatgcgcgac gtgagccacc
    20041 aggtgagcgc gcctccgccg agaccggcga tcagtgcgag cggcgcgcgt ccgatgaagt
    20101 tgcgcaggaa gctcaaggcc gcaaagcccg cgaacaacag gaacggcaat ggcgccccat
    20161 cgctgccgcc cggtttccac gcctgatccg gctctggcag cggttcacca tcgacgactt
    20221 tcagaatctg atcgagcccg gcgttgatgc cgccgaagaa atcgccctgc ttgaacaacg
    20281 gcacgatggt gtcgttgatg atgcggctcg aaatcgcatc cgtcagggac ccttcgagtc
    20341 cgtactgaac ttcgatgcgc agcgcgcgat cgtccttcgc gacgatgagg atggcaccgt
    20401 catcgacatt cttgcgcccg actttccatg cctccgcaag cctgaccccg aactgtgcga
    20461 tgtcctcggg ctgggtggtc gggaggatca gcaacgccac ctgcgagccc ttgcgcgcct
    20521 ggaacgcggt gagcttttca tcgagcgccg actgctgcgc ggccgtaagc gtgccggtaa
    20581 ggtcgactac cagcgcctcg aacttcggta ccggaatctg cgcgcgcacc gccggcgctt
    20641 ggcccgcgag cagcaacgcc agcgtaagca gcgcggctct cacgtgttca gtttgctggc
    20701 ggaggtgtgg cgggagccgg cgcggcttcc accggagttg ccgactgtga aggcgtcgat
    20761 gtgtcgaagg tcacggtggg cgccgtggaa atcgcg
//
LOCUS       DASQJS010000103         4985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_996665, whole genome shotgun sequence.
ACCESSION   DASQJS010000103 DASQJS010000000
VERSION     DASQJS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996665"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1080
                     /locus_tag="VEW08_04175"
     CDS             127..1080
                     /locus_tag="VEW08_04175"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HYJ39963.1"
                     /translation="MTQQTRPNGTLRHLLTLEGMSRPAIEALLTRAQKFVKPVGQPPV
                     HSQSLAGVTVANMFTEPSTRTRVSFELAAKRLGADVVNLEMQLSSRVKGESMLDTIFT
                     LEACHVDVFVIRDAESGVPGMVASNVQPHASVLSAGEAHVAHPTQGLLDALTILQAKK
                     KFDGLTVAIVGDVKHSRVARSAWHVLATLGVAEIRIVSPRGLAPDASEFAGCERHTSI
                     DTGIAGADVIMMLRIQKERMATAAIPDADRYFAKFGLSSERLAKAKPDAIVMHPQPMN
                     RGIEIASDVADGPQSVIRDQVRNGVAVRMAVLEAVIEGRKS"
     gene            1077..1994
                     /locus_tag="VEW08_04180"
     CDS             1077..1994
                     /locus_tag="VEW08_04180"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HYJ39964.1"
                     /translation="MSALPQEEPARSKGVHPAHRGSIFLEDGAVIAQETWPGEQFVLR
                     ISAPKCAARAEPGSFVHLTCDPDVPMRRPLSIQRVNATEGWIEILYKIVGPGLRALSR
                     RHKGDVISVLGPIGQPFRPDPKRPRALLVGGGVGIPPMVFLAEWMSSQGAPWKPLVLM
                     GSEIPFPFKARPSTILVPGIPAGSIAAMPLLDGWGVPSRLASKAGFAGCYDGFVTQLA
                     DEWLGSLDQAVKTEVEVFSCGPTPMLEAMAKVAQKHGVACQVSLEEFMACAVGGCAGC
                     TVKVMTPDGPAMKRVCVDGPVFDAKAVFA"
     gene            2112..3557
                     /locus_tag="VEW08_04185"
     CDS             2112..3557
                     /locus_tag="VEW08_04185"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HYJ39965.1"
                     /translation="MEVLTGRTRAATTITALLILFSVYAPAASAQTFAKGADVSWITQ
                     MEASGYRWQNDNGQQQDLLQILKDHGMNTIRLRVWVNPSGGWNGVNDVVAKAVRAKNA
                     GFRIMIDFHYSDSWADPGQQTKPAAWRTHTFEQLMADVYQHTFNTMNTLATNGIFPEW
                     VQVGNETNNGMLWEDGRASANMRNYAWLVNSGYDAVKAVSPNSKVIVHVSNAYDNALF
                     RWNFDGLRANGARWDIIGMSLYPEPGNWQTLNSQALANMNDMRSRYGKGVMIVEVGMS
                     WDQATASKAFLQDIIARTRNAGGLGVLYWEPQTYNWQGYSKGAWNTNGRPTIALDAFI
                     EGSTPPPGGSVTLQENLTGFCGVDGTLDSNHAGFTGTGFANTSNALNTTVRWRVNAAA
                     AANYTFQWRFANGSTGNRPGNLRINGTTGANVTLQATGAWTTWTNSGSFTAALRAGAN
                     DIALVASTSGGLANIDSMTVTGTGVSVSACN"
     gene            3670..4356
                     /locus_tag="VEW08_04190"
     CDS             3670..4356
                     /locus_tag="VEW08_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYJ39966.1"
                     /translation="MAKARLTLSIALIGILNAAFAETPNPRLAGNQEFVDAAQLEPDL
                     KRGAGFYQTCAACHGVDGSGTPDGEVPAIAGQHGSVLLKQLTDFRHEQRRDERMQHFS
                     DREHLPGAQELTDVAAYVASLPRFPPSAKSIGDGTSLGEGASVYFRECERCHGPLGQG
                     NLLLRRPRLAGQHYDYLLRQLAETAAGRRPGMDSAHVTRLKNLSPAQLRGVADYLSRV
                     SPDLSPTSRR"
     gene            complement(4402..>4985)
                     /locus_tag="VEW08_04195"
     CDS             complement(4402..>4985)
                     /locus_tag="VEW08_04195"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HYJ39967.1"
                     /translation="FINLPDRDLAYLVEGTEHFDDYVEAMTWAQDYAAENRRAMMDAV
                     LRVMREELRPFAMGEVAVNCHHNYCTRERHFGEELIVTRKGAVSAKQGELGIIPGSMG
                     ARSYIVRGLGNKESFESCSHGAGRVMSRTAARARFTIADHEAATAGVECRKDKDVIDE
                     TPAAYKDIDAVMEAQRDLVEIAYTLKQVLCVKG"
BASE COUNT          935 a         1647 c         1573 g          830 t
ORIGIN      
        1 ggacgccgaa gaccgacttc gctcccagcg cgcgagcggc gagcgcaagc ggcgcgtaac
       61 gcgcgaagac gtggatgcgg cggccgccgc gataattctg gaacgctggt tcgaaaaaca
      121 agagacatga cgcaacagac ccgcccgaac ggcacgctac gccacctgct caccctcgaa
      181 gggatgtccc gtcccgccat cgaagccctg ctcacgcggg cgcagaagtt cgtgaaaccc
      241 gtgggccagc cgcccgtgca ctcgcagagc ctggccggtg tcaccgtggc caacatgttc
      301 accgagccgt cgacacgcac gcgtgtgtcg ttcgagctcg cggccaagcg gctcggcgcc
      361 gacgtcgtca atctcgagat gcagctctct tcccgcgtga agggcgaaag catgctcgac
      421 accattttca cgctggaagc ctgccacgtg gacgtgttcg tcatccgcga tgccgaatcg
      481 ggtgtaccgg gaatggtcgc ttcgaacgtg cagccgcacg caagcgtgtt gtcggctggc
      541 gaagcgcacg tggcgcatcc cacccagggg cttctggacg cgctcaccat ccttcaggcg
      601 aagaagaaat tcgatgggct cacggtggcc atcgtcgggg acgtgaagca ctcgcgcgtg
      661 gcgcgttccg cctggcatgt gctcgctacg ctcggggtcg ccgaaatccg catcgtctcg
      721 ccgcgtggcc tcgcgcccga tgccagcgag ttcgcgggct gcgaacgtca tacgtcgatc
      781 gacaccggca tcgccggcgc cgacgtcatc atgatgttgc gcatccagaa agaacgcatg
      841 gcgacggccg cgattcccga cgccgatcgt tacttcgcca agttcggcct gtcttccgag
      901 cgcctggcaa aagccaagcc cgatgccatc gtcatgcatc cgcagccgat gaaccgcggc
      961 atcgagattg cctccgacgt cgccgacggc ccgcagtcgg tgatccgcga ccaggtgcgc
     1021 aacggcgtgg ccgtgcgcat ggctgtgctc gaggccgtca tcgagggcag aaaatcgtga
     1081 gcgccctacc ccaggaagag cctgcccgca gcaagggcgt tcaccccgcg caccgcggca
     1141 gcatctttct cgaagacggc gcggtgattg cgcaggaaac ctggccgggc gagcagttcg
     1201 tgttgcgaat ttccgcaccg aaatgcgccg cgcgcgccga gcccggcagt ttcgtgcatc
     1261 tcacctgcga tccggacgtg cccatgcgcc gcccgctgtc gatccagcgc gtgaatgcca
     1321 ccgaaggctg gatcgagatt ctctacaaga tcgtcgggcc cggtctgcgc gccctgagcc
     1381 gccggcacaa gggcgacgtc atcagcgtac tgggtccgat cggccagccg ttcaggcccg
     1441 accccaaacg acctcgcgcc ctgttggtgg gtggtggcgt gggcattccg ccgatggtgt
     1501 tcctggctga gtggatgagc tcgcagggtg cgccgtggaa gccactggtg ctcatgggtt
     1561 cggagatccc atttccgttc aaggcgcggc catcgaccat tctcgtgccc ggcatccctg
     1621 ccggcagcat cgccgcgatg ccactgctcg atgggtgggg cgtgccgagc cgactcgcca
     1681 gcaaggcggg attcgccggt tgctacgacg gcttcgtcac gcagctcgcg gatgagtggt
     1741 taggctcgct cgaccaggcc gtgaagaccg aagtcgaagt gttctcctgc ggccccactc
     1801 ccatgctcga agcgatggcg aaggtcgcgc agaaacacgg cgtcgcctgc caggtgtcgc
     1861 tcgaagaatt catggcctgc gccgtcggcg gttgcgcggg ttgcaccgtg aaggtgatga
     1921 cgcccgatgg cccggcgatg aagcgcgtgt gcgtcgacgg accggtgttc gacgcgaagg
     1981 ccgtattcgc ctgaaactgg tctgcggatg atcgcaccgc actaacaacg gcactggcgt
     2041 cccggcggcc atccatgtta tttatacgat taatggcctg aggggcctga aggaccgaac
     2101 caggaggcaa catggaagtg ctgacaggcc gcacgcgcgc ggcaacaaca attaccgccc
     2161 tgctcatact cttctctgtc tacgcgcccg ccgcgtcggc tcagaccttc gccaaggggg
     2221 cggacgtcag ctggatcacg cagatggaag ccagcggcta tcgctggcag aacgacaacg
     2281 gccagcagca ggacctgctg cagatcctca aggatcacgg catgaacacg attcgcctgc
     2341 gcgtatgggt gaatccttct ggtggctgga acggcgtgaa cgacgtcgtc gcgaaggcag
     2401 tccgcgctaa aaacgccggc ttccgcatca tgatcgactt ccactacagc gactcctggg
     2461 cggaccccgg ccaacagacc aagcccgccg catggcgcac gcacaccttc gagcagctca
     2521 tggccgacgt gtaccagcac acgttcaaca ccatgaacac gctcgccacc aacggcatct
     2581 ttccggaatg ggtgcaggtc ggaaacgaga ccaacaacgg catgttgtgg gaagacggcc
     2641 gcgcctcggc aaacatgcgc aattacgcgt ggctggtgaa cagcggctac gacgcagtca
     2701 aggccgtgag ccccaactcg aaggtcatcg tgcacgtctc gaacgcctac gataacgcgc
     2761 tgttccgctg gaacttcgac gggctgcgcg cgaacggcgc gcgttgggac atcatcggca
     2821 tgtcgctgta cccggagccc ggcaactggc agacactcaa tagccaggca ctcgccaaca
     2881 tgaacgacat gcgttcgcgc tacggcaagg gcgtgatgat cgtcgaggtg ggcatgtcgt
     2941 gggatcaggc cacggccagc aaggctttcc ttcaggacat catcgccagg acacgcaacg
     3001 cgggtggact cggcgtgttg tattgggagc cccaaactta caactggcag ggctacagca
     3061 aaggcgcctg gaacacgaat ggccggccca ccatcgcgct cgatgcattc atcgaaggca
     3121 gcacgccgcc gccgggcggc agcgtcacgc tgcaggaaaa cctcacgggt ttctgcggcg
     3181 tcgacggcac gctcgacagc aaccatgcgg gctttacggg caccggcttt gcgaacacca
     3241 gcaatgcgct gaataccacg gtgcgctggc gggtgaatgc ggccgctgcg gccaactaca
     3301 cgttccagtg gcgcttcgcg aatggcagca ccggcaatcg ccctggcaac ctgcgcatca
     3361 acggcaccac aggagcgaac gtcacgctgc aggccacagg cgcgtggacc acgtggacca
     3421 actccggctc gttcactgcc gcgctgcgcg ccggcgcgaa tgacatcgcg ctggtcgcga
     3481 gcaccagcgg tgggctggct aacatcgact ctatgacggt gacgggaacg ggagtcagcg
     3541 tcagcgcctg caattaggtc gatgacccgc accccgcgcg aaggacgccg acgtgctcgc
     3601 gatacttgcg tattcacata cgcggtttcc tttatcgaac cgcgcgtgcg acacgggtag
     3661 cctgcggcca tggcaaaagc acgactcaca ctgtccatcg ccctgatcgg aattctgaat
     3721 gcggcgttcg cggagacgcc gaatccgcgc ctggctggca atcaggaatt cgtcgatgcc
     3781 gctcaactcg aaccggatct caagcggggc gccggtttct atcagacgtg cgcggcgtgt
     3841 cacggcgtgg acggcagcgg cactccggac ggtgaagttc cggcgatcgc cggccagcat
     3901 ggaagtgtgt tgctcaagca attgaccgac ttccgccatg aacaacgccg cgacgagcga
     3961 atgcagcact tcagcgaccg cgagcacctg cccggcgcgc aggagctgac ggacgtggcg
     4021 gcatacgtgg ccagcctgcc gcgtttcccg ccgagcgcca agagtatcgg cgacggcact
     4081 tcacttggtg agggcgcaag tgtctatttt cgcgagtgcg aacgctgcca cgggcctctc
     4141 ggacagggaa acctgctgct gcggaggccg agacttgccg gccagcacta tgactatcta
     4201 ttgcgccaac tggcggagac ggcggccggg cgccggcctg gaatggacag cgcgcacgtc
     4261 actcgactga agaatctgtc gccggcgcaa ttgcgcggtg tcgccgacta tctctcgcgc
     4321 gtgagccccg acctcagccc gacgtcgcgc aggtagccac cagcaaaaaa aagggcgggc
     4381 cacttgcgtg gctcgccccg ctcacccctt cacgcagaga acctgcttca gggtgtacgc
     4441 gatctcgacg agatcgcgct gtgcttccat caccgcgtcg atgtccttgt acgcggccgg
     4501 cgtttcgtcg atgacgtcct tgtccttgcg gcactcgacg cccgccgtcg cagcctcgtg
     4561 gtccgcgatg gtgaagcgtg cgcgcgccgc ggtacgcgac atcacgcgac ccgcgccgtg
     4621 gctgcagctc tcgaagctct ccttgttccc caggccgcgc acgatgtacg aacgcgcacc
     4681 catggaaccc gggatgattc cgagctcgcc ttgcttcgca ctcaccgcgc ccttgcgggt
     4741 cacgatgagc tcttcgccga aatgccgctc acgcgtgcag tagttgtgat ggcagttcac
     4801 cgccacctca cccattgcga acgggcgcag ctcctcgcgc atcacgcgca gaaccgcgtc
     4861 catcatcgcg cgccggtttt cggcggcgta gtcctgcgcc caggtcatcg cctcgacgta
     4921 gtcgtcgaag tgttcggtcc cttcgacgag atacgcgaga tcccgatcgg gcaggttgat
     4981 gaagt
//
LOCUS       DASQJS010000104         9360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3506636, whole genome shotgun sequence.
ACCESSION   DASQJS010000104 DASQJS010000000
VERSION     DASQJS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3506636"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VEW08_04200"
     CDS             complement(<1..290)
                     /locus_tag="VEW08_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYJ39968.1"
                     /translation="MKMQSRKGREDPEINLIPLIDVSLLLVIFFMLSSTFMQEGRLKI
                     ELPQASLAPTAKQKMDPIVVTVTQSGSYRVNDRELINSSPDTLRAAILEVAGA"
     gene            complement(287..931)
                     /locus_tag="VEW08_04205"
     CDS             complement(287..931)
                     /locus_tag="VEW08_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYJ39969.1"
                     /translation="MWELVRAGGPFMWPIIICSIALVGIVLERLWTLQRKRVLPQELI
                     KKVSELAERNQVNPKVIEALEKNSALGRVFAAALANRHRGREIMMERVEDVGRQVVHE
                     LERFVNSVGTIASISPLLGLLGTVTGIIRAFNAVMLGGMGDPRMLAGGISEALVCTAA
                     GLTVAIPAYVAHRYLRGKVERIVVEMEKIAVNFADSLGPELQRGDSDGGVARSA"
     gene            complement(967..3195)
                     /locus_tag="VEW08_04210"
     CDS             complement(967..3195)
                     /locus_tag="VEW08_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HYJ39970.1"
                     /translation="MPDAGWLLVAAASATLVLVTRLRNFATGRYLAWLLAGMVLASAS
                     TLRWLALCVPVTAADTRVLLEGVIRGVPAREGAELHFDLDAVVVEGQPRDARSRRVRV
                     VWRDTPVAPRAGESWRLLVRLVPLADAHNFSGPDTARFAFRDGVHFAGRVLPSALNAR
                     LTLANTSVDTLRARVAARVGESVADPDAAALLTALAVGLTDRLSADQWRVFNATGTTH
                     LVAISGLHVTMFALVAFTAARFAWRWLPCARRVEREPFAMLLGLGAAGAYALLSGFSV
                     PAQRTWLMLALFAAGRLAARHVGVGRFWSLALMAVLCLDPRASLAAGFWLSFVAVGVI
                     LMAASALAAPPSLATFASCANAVRLQCAILLALAPLTFAVFGGLSLAGLAVNLVAIPL
                     VSFVLVPLVLAGALAVLVAPGLSDVFFKIAAHLHEGFWPALSWAADREFALWNATPAL
                     WWFPFAMLAALVLLRRWPPALRVSATCALLPLVFAPAHVPEPGAARVNVLDAGRGTAA
                     LVFTHSHVLLFDTGDSWNTRGARLRRWVLPALDELRRDRVDLLVLPALDGDRAQGAAV
                     LAFEREVAALLVGGGWQATSLPVRRCADSEFRWDGVLFQSFTAGVGGDYCVLRVSVGP
                     HAILLGGDLDAEAERGLAARVEPGALSSEVVLMSRQASSTASAPEWIEASDAVLAIAT
                     GGIANSHSRGVTLARWRASGTMVLDTRRAGGVELGLGTSGVRVVAVARVSRYPFAWRR
                     SP"
     gene            3286..3804
                     /locus_tag="VEW08_04215"
     CDS             3286..3804
                     /locus_tag="VEW08_04215"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HYJ39971.1"
                     /translation="MPRGLFRKITPRSETLRTHWALKPFGRFFGDPRLWSLQRRTVTP
                     AFGAALAICFVPLPIHIPLAAITAIFCRIHIPTIMLTLMLMNPLTFLPLFLLAYKVGA
                     IVTGAPPSNFHFEMSWDWVQNGLGANWKPFLVGCGLTGALFGLVGWGVLDLLWRANVR
                     KRYRERAGSTSD"
     gene            complement(3805..4494)
                     /gene="lolD"
                     /locus_tag="VEW08_04220"
     CDS             complement(3805..4494)
                     /gene="lolD"
                     /locus_tag="VEW08_04220"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HYJ39972.1"
                     /translation="MNDNERVMEATGVVRAFRQGPIDVQVLQGVNLSVRAGERLAIIG
                     ASGSGKTTLLQILGGLDRPTAGVVKIAGSDIHGLEEKARGELRNRTLGFIYQFHHLLP
                     EFSALENVAMPLLVRRVPRNEAENSARAILQRVGLGERLVHRPHELSGGERQRAAVAR
                     ALVTNPRIVLADEPTGNLDGGNAESVFELMLELNRELKTSLIVVTHDRRLAARMDRIL
                     ELDRGNLVERS"
     gene            complement(4487..5749)
                     /locus_tag="VEW08_04225"
     CDS             complement(4487..5749)
                     /locus_tag="VEW08_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HYJ39973.1"
                     /translation="MRYEWPYEWKIGTRYLRSTHRSGFVSFVASMSVIGLALGVAVLI
                     VVLSVLNGFEHELRSRMLTVTSHATITGIEGEIENWRQAQVDARKEPGVLAVVPFVES
                     RGLLANGERVAGAMVRGILPEEESKAVGLESRMKPGHLSDLEAGKYRVILGSALATEL
                     AVKVGDKVVLMTPEGSATPAGFQPRMKRFTVSGIFESGMYEFDRGLALTHMADAAMLY
                     RKGDRVTGLRLALEDPFLAPLMVRTVARAIDYDGHGYYVNDWTHDHENFFRTIQLTKS
                     MMFFILLILVVVAAINLVATLVMIVKEKQTDIAILRTIGAAPANVLRIFLVQGALIGL
                     VGTLAGACLGWVLALNVTAIIRGIESMFGIKFLDASVYLMSDLPSDVRLGDVLQVAGV
                     ALALSALATIYPAWRAARTLPAEALRHE"
     gene            complement(5746..6996)
                     /locus_tag="VEW08_04230"
     CDS             complement(5746..6996)
                     /locus_tag="VEW08_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HYJ39974.1"
                     /translation="MLNSLPLFVGLRYVRARSHKFFVSFITWVSLLCVCLGVMALIVI
                     LSVMNGLEGELRSRLLALSAHARIFVPAPASATPDWSALADRVRQAPNVTGAAPFIEV
                     EALAVRKPDMLPVRLRGIDPRHEGEVARMTQAIVEGKLADLTPGSNHVIVGRGIAQML
                     ALGVGDPITVLVPTTDANGFPEPRLREFLVTGVFDAAVQDYDGQLLIAALDDVRALFP
                     NADARMSLHVNFKDALTASENSALLARSLPAGVVIRDWTVDHASYFRAIRIEKTMVAI
                     ILMLIVAVAAFYLVAMLAMVVTDKRTDIAILRTLGTSPRRVMAIFLIQGSVIAWFGVL
                     LGVTLGSWLGYYAGAAAAFLERLFRFEIFSSDVYMVTRIPSELRGEQIAWIAGIAMLI
                     TLAATIYPAFRASRVPPADALRYE"
     gene            7139..7426
                     /locus_tag="VEW08_04235"
     CDS             7139..7426
                     /locus_tag="VEW08_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39975.1"
                     /translation="MLARTMKMTVVALALGATVPTFAETLAIDGQVAIKPVGIETPQR
                     GSTMSAVEQKFGAPSNKAGPVGNPAITKWFYPNFVVVFENDKVLHAVVVAG"
     gene            complement(7430..8635)
                     /locus_tag="VEW08_04240"
     CDS             complement(7430..8635)
                     /locus_tag="VEW08_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39976.1"
                     /translation="MSQDFPLEYRMGGSGRRFMWGIGAFAILLGFAGIGYFLFATDEL
                     GDRVLVMALGGFFALLGLPVLASVIRTRVTLYADAIETRGMFVIKRMERADISGRRLI
                     SGGDGPSVIQLLTNTTRVRSLKLPQYIATDAAWDRWFASIPDVDAEERNAALANFLEQ
                     SDVAGSNEEKFAALEQARRNAKWLFYAALAAGGWAWFHPQPYHLSVLCLALVPWVAVA
                     MVSAGPSVYRINLARNDVVADVSAPLMLPGMVLMLRAMLDIEILDWTSVAALSAAATA
                     ILLLILLVFLPELRKNSGSLGMAILMIAPYAYGASMIANVYFDRGEPAKHMTQVAAKR
                     VSTDNGKFFHLKLTPWGPRAESEEVSVGREIYARVATGDSVCVYLFRGAFGVRWFEVW
                     DCPRNEAGG"
     gene            8698..>9360
                     /locus_tag="VEW08_04245"
     CDS             8698..>9360
                     /locus_tag="VEW08_04245"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HYJ39977.1"
                     /translation="MPALLKPPIPAARSSQRWHQLYGSARSLALAEAVEADTRPYLVI
                     ARDARELDQLRSELAFFLGDAKSIHVLPDWEVLPYDLFSPHPDIISERLAALAELTRL
                     KSGVLLAAADTLGQRIAPRGYVDGRTFNLAVGDKLPIEPLRARLVESGYASVSQVSAP
                     GEFALRGSLFDVFPMGAETPLRVDLFDDEIEVIREFDPDTQRSGDSLKHVRLLPGREM
                     PLD"
BASE COUNT         1820 a         3132 c         2932 g         1476 t
ORIGIN      
        1 gcgccggcga cttcgaggat ggctgcgcgc agcgtgtcgg gcgaggaatt gatcagctcc
       61 cgatcattca cgcggtaact gccactctgg gtcacggtga cgacgatcgg atccattttc
      121 tgcttggccg tcggcgcaag acttgcctgc ggcagctcga tcttgagccg gccttcctgc
      181 atgaaggtgg acgacaacat gaagaagatg accagcagca gcgagacgtc gatcaacggg
      241 atcaggttga tctcaggatc ttccctgccc ttgcgggact gcatcttcat gcactgcgag
      301 cgacgccacc gtcgctgtct ccgcgctgca gttcggggcc cagggagtcg gcgaaattca
      361 ccgcgatctt ttccatctcg acgacgatgc gctcgacctt cccgcgcagg taacggtgcg
      421 caacgtaggc cggaatcgcc accgtgagac cggccgcggt gcagaccagg gcctccgaga
      481 taccacccgc cagcatgcgc ggatcaccca tgccaccgag catcaccgca ttgaacgcgc
      541 gaatgatgcc cgtcaccgta ccgagcagtc ctaacaacgg cgaaatgctg gcgatggtac
      601 ccacggagtt cacgaagcgt tccagctcgt gaaccacctg gcggcccacg tcttcgacgc
      661 gttccatcat gatctcgcga ccacgatggc ggttggccag cgccgccgca aaaacgcgcc
      721 ccagcgccga gttcttttcg agtgcctcga tgaccttggg gttgacctga ttccgctcgg
      781 cgagctcgga caccttcttg atgagctcct ggggcagcac gcgcttgcgc tgcaaggtcc
      841 agagcctttc gagaacgata ccgacgagcg cgatcgaaca gatgatgatc ggccacatga
      901 aagggccgcc ggcccgtacg agttcccaca tggcggggat cataccggag ggcgcggccg
      961 aaagggtcac ggcgagcgcc gccacgcgaa ggggtaacga gacacgcgcg cgacggcgac
     1021 cacgcgcacg cccgacgtcc ctaaaccgag ctcgacaccg cctgctcgcc gtgtgtcgag
     1081 aaccatggtt cccgaagcgc gccagcgcgc gagcgtcacg ccgcgggaat gtgaattcgc
     1141 aataccgccg gtcgcgatcg ccagtaccgc gtcgctcgct tctatccact ctggcgccga
     1201 cgccgtcgag ctcgcctgtc ggctcatgag caccacctcg ctcgagagcg caccgggctc
     1261 gacgcgcgcc gccaggccgc gttccgcctc ggcatcgaga tctccgccta gcagaatggc
     1321 gtgtgggccc accgagacgc gcaggacgca gtagtccccg cccacacctg cggtgaaact
     1381 ctggaacagg accccgtccc agcgaaactc cgaatcggca caccggcgaa ccgggagcga
     1441 agtcgcctgc caccctccgc cgaccaggag tgccgcgacc tcgcgttcga atgccagcac
     1501 cgcggctccc tgcgcacggt caccatcgag cgcgggcaat accagcaggt cgacgcggtc
     1561 acgccgcagt tcgtcgagtg ctggcagcac ccagcgtcgc aggcgcgcac cccgcgtgtt
     1621 ccagctgtca cccgtatcga agagcagcac atgtgaatgc gtgaacacca gcgcggccgt
     1681 gccccgcccc gcgtcgagca cattgacccg cgccgcgccg ggctcgggca cgtgtgcagg
     1741 cgcgaacacc aatggcagta gcgcgcaagt ggccgagacc cgcaacgccg gcggccagcg
     1801 acgcagcagc accagcgccg ccagcatcgc gaatggaaac caccacaatg ccggcgtggc
     1861 gttccacagc gcgaactcgc ggtctgcggc ccaggagagc gcgggccaga acccttcgtg
     1921 gagatgagcc gcgatcttga agaaaacgtc gctcaatccc ggcgcgacca gcaccgcgag
     1981 cgcaccagcc agcaccagcg gcaccagcac gaacgagacg agcgggatcg caacgagatt
     2041 gaccgcaagc cccgccagcg aaagcccacc gaacaccgcg aacgtgagtg gcgcgagcgc
     2101 caacaggatg gcgcattgca ggcgtacggc gttcgcgcag ctcgcaaacg tggccaggga
     2161 aggcggcgct gccagcgcgc tggctgccat gaggatgacg ccgaccgcca cgaaagacag
     2221 ccagaagccg gcggccagcg atgcccgcgg atcgaggcac aacacggcca tcaacgcgag
     2281 cgaccagaaa cggccaacac cgacatgccg tgccgccagc cggccggctg cgaacaatgc
     2341 gagcatcagc caggtgcgct gcgccggtac cgagaatccg gatagcagcg cgtaggcgcc
     2401 cgcggcgccg aggcctaaca acattgcgaa aggttcgcgt tcgactcgtc gtgcgcaagg
     2461 cagccagcgc cacgcgaagc gcgccgccgt gaacgccacg agcgcgaaca tcgtgacgtg
     2521 caacccggag atggcgacca ggtgcgtggt gcccgtggca ttgaacacgc gccattggtc
     2581 ggcactcaac cgatcggtga gccccaccgc cagcgccgtg agcagcgccg ccgcgtcggg
     2641 atcggcaacc gattcgccga cgcgggccgc cacgcgggca cgcagagtgt cgaccgaagt
     2701 atttgccagc gtgagccggg cgttcagtgc cgatggcaac accctgcccg cgaaatggac
     2761 gccatcgcgg aaagcaaaac gcgcggtatc ggggcccgaa aaattgtgcg cgtcggcgag
     2821 cggcaccagc cgcaccagca ggcgccagga ttcaccggct cgcggcgcca cgggcgtatc
     2881 acgccatacg acccgcacgc gccgcgaacg tgcatcccgc ggctggccct cgacgacgac
     2941 ggcatccagg tcgaagtgca gttccgcgcc ttcgcgcgca ggaactccgc gaatgacgcc
     3001 ctcgagaagc acgcgcgtgt ccgccgccgt caccggcacg caaagcgcca gccagcgaag
     3061 cgtggacgcc gaggcgagca ccatgccggc cagcaaccag gcgagatacc gccccgttgc
     3121 gaaattcctc agccgcgtga cgaggaccag cgtggcgctg gcggcggcaa ccagcagcca
     3181 accagcgtca ggcacagccg cggagaacaa gcccagaaga acgcccgaca gcagtgcaag
     3241 cgcgccgatc acgcaaggcg gcgtcgggtg ctagagtgca tcccgatgcc acggggactg
     3301 tttcgcaaaa tcacgccacg cagcgaaacc cttcgcacgc attgggcgct aaaacccttc
     3361 ggtcggtttt tcggtgaccc gcgcctgtgg tcactgcaac ggcgcacggt aaccccggca
     3421 tttggcgcgg ccctcgccat ttgtttcgtg ccgctgccca ttcacattcc gctggcggcg
     3481 atcacggcca tcttctgccg cattcacatc cccaccatca tgctgacgct gatgttgatg
     3541 aatccactga cgttcttgcc gctcttcctg ttggcataca aagtgggcgc catcgtcacc
     3601 ggcgcgccac ccagcaattt ccatttcgag atgagttggg attgggtaca gaacggcctg
     3661 ggtgccaatt ggaaaccctt cctcgtgggc tgtggcctga ccggcgcact gtttggcctg
     3721 gtgggctggg gcgtgctcga cctgctctgg cgtgccaacg tgcgcaagag atatcgcgaa
     3781 cgcgccggtt ccaccagcga ctaatcagct gcgttccacc agattcccgc ggtccagctc
     3841 caggatgcga tccatgcgcg cggccagtcg ccggtcgtgc gtgacgacga tcaggctggt
     3901 cttgagttca cgattcaact cgagcatgag ctcgaagact gattcggcat tgccaccatc
     3961 gagatttccg gtgggctcgt cggcaagcac gatgcgtgga ttggtgacga gcgcgcgtgc
     4021 gacggcagcc cgctgacgct cgccgcccga caattcatgc ggacggtgga ccaggcgctc
     4081 gccgagaccc acgcgctgca ggatcgcccg cgcgctgttt tccgcctcgt tccgcggcac
     4141 gcgccggacc agcaatggca tcgccacgtt ttccagtgcc gagaattccg gcagcaggtg
     4201 atgaaactga tagatgaatc cgagcgtgcg gttgcgcaat tctccgcgcg ccttctcttc
     4261 gagcccatga atgtcgctgc cggcgatttt cacgacacct gcggtcggac ggtcgaggcc
     4321 accgagaatc tgcagcaacg tggtcttgcc ggagcccgac gcgccgatga tcgccaggcg
     4381 ttcgccggcg cgaacgctca ggttcacgcc ctgcagcacc tgcacgtcga ttggcccctg
     4441 tcggaacgcg cgcacgacgc ccgtggcctc cataacgcgt tcgttgtcat tcatgacgaa
     4501 gcgcctccgc cggcagcgtg cgcgcggcgc gccacgcggg gtagatagtg gccagcgccg
     4561 agagcgccag ggccacgccc gccacttgca atacatcgcc gagacgcacg tcggaaggca
     4621 gatcgctcat gaggtagaca ctcgcatcca ggaactttat accgaacatg ctctcgatcc
     4681 cgcgaatgat cgccgtgaca ttgagcgcca gcacccagcc aaggcacgcg cccgccaatg
     4741 tgccgaccag gccgatcaac gcgccctgca ccaggaagat ccgcagcacg ttggccggcg
     4801 ccgcgccgat ggtgcgcagg atcgcaatgt ccgtctgctt ttccttcacg atcatcacca
     4861 gcgtcgccac caggttgatc gccgcgacca cgaccaggat cagcaggatg aaaaacatca
     4921 tggacttcgt gagctggatg gtgcggaaga aattctcatg atcatgggtc cagtcgttga
     4981 cgtagtagcc gtggccgtcg tagtcgatgg cgcgcgccac ggtgcgcacc atgagcggcg
     5041 cgaggaacgg atcttcgagc gcgaggcgca gacccgtcac gcggtcgccc ttgcggtaca
     5101 gcatcgcggc atcggccatg tgcgtgagcg cgagtccgcg atcgaactcg tacatgccgg
     5161 attcgaagat gccactcacc gtgaagcgct tcatgcgcgg ctggaaaccc gccggggtgg
     5221 cgctgccctc gggcgtcatg agcaccacct tgtcgccgac cttcacggca agttcggttg
     5281 ccagggcgct gccgaggatc acgcgatatt tccctgcttc gagatcggac aggtggcccg
     5341 gcttcatgcg gctctcgagg ccgacggcct tgctctcctc ttcgggcagg atgccgcgca
     5401 ccatggctcc ggccacgcgc tcgccattgg ccaggagacc acgtgactcg acgaacggaa
     5461 ccaccgccag aacgcccggt tccttccggg cgtcgacctg cgcctggcgc cagttttcta
     5521 tttcgccttc gatgccggtg atggtcgcgt gcgaagtcac ggtcagcatg cggcttcgca
     5581 actcgtgctc gaacccgttg agcaccgaca acacgacgat cagcaccgcg acgccgagcg
     5641 ccagcccgat gaccgacatg gacgcgacga acgacacgaa tccgctgcga tgcgtggagc
     5701 gcaggtagcg cgtgcctatc ttccattcat agggccattc gtacctcatt cgtatctcag
     5761 cgcgtcggcc ggaggaacgc gcgaggcgcg aaacgccggg tagatagtgg cggcgagcgt
     5821 tatcagcatg gcgatgccgg cgatccaggc aatctgttcg ccgcgcagct ccgaggggat
     5881 gcgtgtcacc atgtacacat cggagctgaa gatttcgaag cggaacagcc gctcgaggaa
     5941 ggcagccgcc gcgccggcgt agtaccccag ccatgagccg agagtgacgc ccagcagcac
     6001 gccgaaccag gcgatgacgc tgccctggat gaggaatatc gccatcaccc ggcgcggcga
     6061 agtgccgagt gtgcgcagga tggcgatatc ggtgcgcttg tcggtaacca ccatcgcgag
     6121 catggccacg aggtagaacg ccgccaccgc gacgatcagc atgaggatga tcgcaaccat
     6181 ggtcttctct atgcggatcg cacggaagta gctggcgtga tccaccgtcc agtcgcggat
     6241 caccacaccc gccggaagcg atctggcgag caatgccgag ttttcactcg cggtcagcgc
     6301 gtccttgaaa ttcacgtgca gcgacatgcg cgcatctgca ttcgggaaga gcgcgcgcac
     6361 gtcgtcgagc gcggcgatca gcagctggcc gtcgtaatcc tggaccgccg cgtcgaatac
     6421 gccggtgacc aggaattcgc gcaggcgggg ttccgggaat ccgttcgcat cggtggtggg
     6481 cacgagcacc gtgatcggat cacccactcc aagagccagc atctgcgcaa tgccgcggcc
     6541 gacgatgacg tggttcgagc ccggcgtgag gtcggcgagt ttgccttcga cgatggcctg
     6601 ggtcatgcgg gcgacttcgc cctcgtggcg gggatcgatg ccgcgcaggc gtaccggcag
     6661 catgtccggc ttgcgcacgg ccagcgcctc gacctcgatg aacggcgcgg cgcccgtgac
     6721 attcggcgcc tggcgcactc ggtcggcgag cgcgctccaa tccggcgtcg cgctggcagg
     6781 agccggcacg aaaatgcgcg catgtgcgga aagcgccagc agccggctgc gcaactcccc
     6841 ttccaggccg ttcatgaccg acaggatgac gatgagcgcc atcacgccca ggcacacgca
     6901 cagcagcgat acccaggtaa tgaacgaaac gaagaacttg tgggagcggg cccgcacgta
     6961 gcgcaggccg acgaacaggg gcagggaatt cagcattgac cggaattgtg ggcgaaaaac
     7021 ctgacgggga tcacaaaagc actgttagtt tgggtcggta catggcgaca aggctgactt
     7081 tgccacagcg gggggtatat tgccgccacg gtcgagtcgc cggccgcagg agaaagccat
     7141 gcttgctcgc acaatgaaga tgacagtggt cgccttggcg ctgggagcca cggtccccac
     7201 attcgctgag acgctggcca tcgacggcca ggtcgccatc aagcccgtgg gaatcgaaac
     7261 cccgcaacgc ggctcgacca tgagcgcggt cgaacagaaa ttcggtgcgc cgtccaacaa
     7321 ggctggcccg gtcggcaatc cggccatcac caagtggttc tacccgaact tcgtggtggt
     7381 gttcgagaac gacaaggtgc tgcacgcggt ggtcgtcgcg ggctgaaatt cagcccccgg
     7441 cttcattccg ggggcagtcc cacacctcga accagcggac gccgaacgcg ccgcggaaca
     7501 ggtacacgca taccgagtcg ccggtcgcga cacgcgcgta gatttcccgc cccacactta
     7561 cctcttcgga ctcggcacgc gggccccagg gtgtgagctt caaatggaag aatttcccgt
     7621 tgtccgtgct gacccgtttg gccgcgacct gcgtcatatg tttcgccggc tcaccacggt
     7681 cgaagtacac attcgcgatc atcgacgcgc cgtaggcata gggggcgatc atcaggatcg
     7741 ccattcccag gctgccgctg ttcttgcgca gctctggaag gaataccagc aggataagca
     7801 gcaggatcgc cgttgccgcc gccgacaacg ccgccacgct ggtccagtcg agaatctcga
     7861 tatcgagcat cgcgcgcaac atcagcacca ttcccggaag catcaacggc gcagacacgt
     7921 cggcaacgac gtcgtttcgc gcgagattga tcctgtagac agagggaccg gctgacacca
     7981 ttgctacggc tacccagggc accagtgcca ggcaaagcac ggagaggtgg tagggctgcg
     8041 gatgaaacca ggcccaccct ccggctgcga gcgccgcgta gaacagccat ttcgcattgc
     8101 gcctggcctg ctcgagcgcg gcgaactttt cttcattcga gcccgcgaca tcggattgct
     8161 cgaggaagtt tgcaagcgcc gcattccgct cctccgcatc gacgtcaggg atcgaagcga
     8221 accaacggtc ccacgcggcg tcggtcgcga tgtattgcgg caacttgagc gatcgcactc
     8281 gcgtggtgtt cgtcagcagc tgaatcaccg atggtccgtc gccgcccgag atcaatctgc
     8341 gcccggatat gtcggcacgc tccatgcgct ttatgacgaa cattccgcgc gtctctattg
     8401 catcagcgta gagcgtcaca cgtgtgcgaa tgacggacgc aagtaccgga agcccgagca
     8461 gtgcaaagaa tccaccgagc gccatgacga ggactcgatc ccccagttca tccgtggcga
     8521 acaggaaata gccaatgccg gcgaagccga gaagaatggc gaaggcaccg atcccccaca
     8581 tgaagcggcg ccccgaaccg cccatgcggt attccagtgg aaaatcctga ctcaagccgc
     8641 tccctgaaag gaaatttgca ctgacgggcc cgaagggtaa gctgccgcgc tttcgcaatg
     8701 ccagccctcc tcaaacctcc tattcccgcc gcgcgatcga gccagcgctg gcaccagctc
     8761 tatggctccg cgcgcagcct tgcgctggcc gaagccgttg aagcggacac ccggccctac
     8821 ctcgtcatcg cccgtgatgc gcgcgagctg gatcagcttc gttcggagct ggcgttcttc
     8881 ctcggggacg cgaagtcgat ccatgtcctg cccgactggg aagtgctgcc ctacgacctg
     8941 ttttcgccgc accccgacat catttccgag cgcctcgcgg cgcttgccga gctgacccgg
     9001 ctgaagtcgg gggtgttgct ggccgcggcc gacacgctgg gtcagcgcat cgcccctcgc
     9061 ggctatgtgg acgggcggac tttcaatctc gccgtcggtg acaaactgcc catcgagccg
     9121 ctgcgtgcgc gattggtgga atccggctat gcctcggtgt cgcaggtcag cgcacccggg
     9181 gagttcgcgc tgcgcggatc gttgttcgat gtattcccga tgggcgcgga gacgccgttg
     9241 cgtgtcgatc tcttcgacga tgagatcgag gtgatccgcg agttcgatcc ggacacgcag
     9301 cgctccggcg attcattgaa acacgtgcgc ctgctgcccg gccgcgagat gccgctggat
//
LOCUS       DASQJS010000105         4962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_997110, whole genome shotgun sequence.
ACCESSION   DASQJS010000105 DASQJS010000000
VERSION     DASQJS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997110"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VEW08_04250"
     CDS             <1..1081
                     /locus_tag="VEW08_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ39978.1"
                     /translation="YPARNLLGVDYHLSNDTTIYLEYEASEGDQLATDMTRVGVRTRP
                     WERTQVNSSINTQATEFGPRTFANFGLTQGFRFREHWAFDVGVDQTNTLRGPDLEPLN
                     PNAPLASGSMTDDFFAAFVGAAYHQELWQMTSRLEHRNSDNEERWSSTTGWYREPVAG
                     HAMSVSLQAFDMSAALGESTEAVGRFAWAFRPDSSNWIVFDRLEMKFDERSDLQGSFD
                     SGRLVNNLHANWQLNPALQLGLQYGVRYVSSTFEDEKYSGVSDILGFDLRRQLTRRFD
                     LGVHAAALHSWESAVMDYSTGFDVGATIAKNIWVSVGYNFAGFRDDDFSASRHTAQGP
                     YVKIRIKADQDTFKDLSLDSLRPSR"
     gene            1124..3103
                     /locus_tag="VEW08_04255"
     CDS             1124..3103
                     /locus_tag="VEW08_04255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HYJ39979.1"
                     /translation="MQPLDEVFGPRGPLARTLPGFTPRRSQLAMAGRVALALENRAPL
                     VVEAGTGTGKTFAYLVPALLSGRRVLISTGTRTLQDQLFNKDLPLVAGAIGVPARVAL
                     LKGRSNYLCTYRLKQLGGQKSLVGTRDRTLARVERWAGTTRTGDLAEVPDLGDAHPLW
                     PQITSTRDNCLGARCADFGKCHVVEARRKAVDADIVIVNHHLLLADLALKEDGFGDLL
                     GTADAVILDEAHQIPDLATQFFGTRLGSRQVELLVRDARQELGVARAPATTLSTELGA
                     VEKALAALAEIFRATPRPDWLAADTPLADAAGDLSRALRDLNGELNEFSREPGIAQCA
                     GRATELATRLDAVVHAEEHEGARSVELTPRGFTLSLLPFDIAARFASMAHGTRAAWVF
                     TSATLSVGEDFAHFTSRLGLADAETLAIPSPFDFETQALLYLPAQLPDPASQAHTGAV
                     VDVAVPLIEASAGGAFVLFTSHRALQRAAQLLRDRWTELADYPLLVQGEAPREQLLRR
                     FRESGNAVLLGTASFWEGVDVKGDALRLVIIEKLPFSSPDDALTKARIEHLKATGGNA
                     FREYQLPEAALALKQGVGRLIRSETDRGVVVICDPRLIDKPYGRVFRASLPPMPITRA
                     GADAEKFLRRIAGTAAVPTGLTRIPEAGEMSDEIA"
     gene            3100..3777
                     /gene="tsaB"
                     /locus_tag="VEW08_04260"
     CDS             3100..3777
                     /gene="tsaB"
                     /locus_tag="VEW08_04260"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HYJ39980.1"
                     /translation="MKILAIDTATEGCSVSLGIGDRSIDRYVELERGHAEQLLPMVDS
                     VLAEGGIALNALDAIAFGRGPGGFTGVRLAASVAQGLAFGAQVGVVPISDLAAVAQRV
                     VQLDSSVKRVLVANDARMKEVYWATFEVDGVLTSGAEHVSAAAAVLLPPDTSGAWAAA
                     GRGLLAWPALAESCRVAGARLHADLLPRASEVLALARLAVAAGQILDPADALPVYVRD
                     RVAQSAL"
     gene            3862..4557
                     /gene="phoB"
                     /locus_tag="VEW08_04265"
     CDS             3862..4557
                     /gene="phoB"
                     /locus_tag="VEW08_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HYJ39981.1"
                     /translation="MSAKQILVVEDERPIREMIAFGLRRAGFEVREAADARTGRAEVA
                     NKLPDLLLVDWMLPDTSGLEFTRALKRDRETRELPVIMLTARAEEGDKVAGLEGGADD
                     YITKPFSPRELLARINAVLRRTTPVEGTERIDVEGLVLDQSSQRVSSGERTVALGPTE
                     YRMLAFFMTHPERVYTREQLLDRVWGGNVYVEERTIDVHIRLLRKALEEFGYDRLIQT
                     VRGSGYRFSSRPL"
     gene            4565..>4962
                     /locus_tag="VEW08_04270"
     CDS             4565..>4962
                     /locus_tag="VEW08_04270"
                     /inference="COORDINATES: protein motif:HMM:NF023236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor protein PhoR"
                     /protein_id="HYJ39982.1"
                     /translation="MNSGSTAFLYVALRVLAAVALGAVLGWFAGSVWIGVSGVLSLYL
                     AWNLWQIRALQFWLQHRSVADPPDAPGLWGDVVAQVVRLHRRKRFHKERLTRLFRELR
                     RSTAAMPDGVVMLDPMGEIIWFNRKAGEML"
BASE COUNT          808 a         1638 c         1641 g          875 t
ORIGIN      
        1 ttacccggcc cgcaacctgc tcggtgtcga ctatcacctg tcgaacgaca ccacgatata
       61 cctggagtac gaagcgtcgg agggtgacca gctcgcaacg gacatgaccc gcgtcggtgt
      121 gcgcacgcgt ccgtgggagc gcacgcaggt gaattccagt atcaataccc aggccaccga
      181 attcggcccg cgcacgtttg cgaatttcgg cctgacccag ggcttccgct tcagggaaca
      241 ctgggccttc gatgtcggcg tcgatcagac caacacgctg cgcggcccgg atctcgaacc
      301 gctcaatccg aatgcaccgc tcgcttcggg ttcgatgacg gacgacttct tcgccgcgtt
      361 cgtgggtgcg gcgtatcacc aggagctgtg gcagatgacg agccggctcg agcatcgcaa
      421 ttccgacaac gaagagcgct ggagttcgac gacgggctgg tatcgcgagc cggtggccgg
      481 tcatgcgatg tcggtgtcac tgcaggcctt cgatatgagt gccgcgctcg gcgagagcac
      541 cgaagccgtg ggccgtttcg cctgggcctt ccgccccgat tcgagcaact ggatcgtctt
      601 cgaccgcctc gaaatgaagt tcgacgaacg cagcgacctc cagggttcgt tcgactccgg
      661 gcgcctcgtc aacaatctgc acgccaactg gcagttgaac ccggcgctgc agcttgggct
      721 ccagtacgga gtgcgttacg tgtcgagcac gttcgaagac gagaaatact ccggcgtttc
      781 cgacatcctc ggcttcgacc tgcgtcgcca gctgacgcgc cgcttcgacc tcggggtgca
      841 tgccgcggcg ctgcattcct gggaatccgc agtcatggac tactccaccg gattcgacgt
      901 gggcgcgacg atcgcgaaga acatctgggt ctcggtgggc tacaacttcg cgggattccg
      961 cgatgatgac ttctcggcca gccgccacac ggcgcagggt ccgtacgtca agatccgcat
     1021 caaggctgac caggatactt tcaaagattt gagcctcgac agcctgcgcc cgtcgcgcta
     1081 ggttggctgc cgtccgccga tacggcggca gatcaattac ctgatgcaac cactcgacga
     1141 ggtattcggc ccgcgcgggc ccctggcgcg cacgctgccg ggcttcacgc cgcgccgttc
     1201 gcagctcgcg atggccggac gggtggcgct cgcgctggaa aaccgcgccc cgctcgtggt
     1261 cgaggccggc accggcaccg gcaagacctt tgcctacctc gtgccggcgc tgctctccgg
     1321 caggcgcgtg ctcatttcca cgggcacgcg caccctgcag gaccagctct tcaacaagga
     1381 cctgccactg gtggccggcg ccatcggcgt gccggcgcgc gtcgcgttgc tcaagggccg
     1441 ctctaactat ctgtgcacct accggctgaa gcagctcggc gggcagaaga gcctggtggg
     1501 tacgcgcgat cgcacgctgg cgcgtgtcga gcgctgggcc gggaccacgc gcacgggtga
     1561 tctcgccgaa gtgccagacc tgggcgacgc ccacccgctg tggccgcaga tcacgtcaac
     1621 cagagacaac tgtctcggcg cgcgttgcgc cgatttcggc aagtgccacg tcgtcgaggc
     1681 tcgccgcaag gccgtcgacg ccgacatcgt catcgtgaat caccatctgc tgctggcgga
     1741 tctggccttg aaagaagacg gtttcggaga cctgctcggc acggcagacg cggtcattct
     1801 cgacgaagcg caccagattc ccgaccttgc cacgcagttt ttcggtacgc gcctcggcag
     1861 ccgccaggtg gagctgctcg tgcgtgacgc gcgccaggaa ctgggtgtgg cccgggcgcc
     1921 agcgacgacg ctgtccaccg agctcggcgc cgtcgagaag gcgctggcgg cattggccga
     1981 aattttccgc gccacgccgc ggccagactg gctggctgcc gacacaccgc tggcggacgc
     2041 cgccggggat ctttcgcgag cgcttcgcga cctcaacggc gagttgaacg aattcagccg
     2101 cgagccgggc atcgcgcagt gcgcggggcg cgccaccgag ctcgccacgc gtctcgatgc
     2161 ggtggtgcac gccgaagagc acgaaggtgc gcgcagcgtc gagctcacgc cgcgcggctt
     2221 cacgttgagt ttgctgccat tcgacatcgc ggcgcgtttc gcatccatgg cccacggcac
     2281 gcgcgcggcc tgggtgttca cttcggccac gctgtcggtg ggcgaagact ttgcgcattt
     2341 cacgtcccgc ctggggctgg ccgacgccga aacgctcgcc attccgagtc cattcgattt
     2401 cgagacgcag gcgttgctct atctacccgc gcagctgccg gacccggctt cccaggcgca
     2461 caccggcgcc gtcgtcgacg ttgcggtgcc gctcatcgaa gcgtcggccg gcggcgcctt
     2521 cgtgctgttc accagccacc gcgcattgca gcgtgccgcg cagctgctgc gtgaccgctg
     2581 gaccgagctt gccgactatc cactgctggt gcagggtgag gcgccgcgcg aacaactgtt
     2641 gcggcgcttc cgcgagtcgg gcaacgccgt gctgctcggc accgcgagtt tctgggaggg
     2701 cgtcgacgtc aagggcgacg cgttgcggct ggtcatcatc gagaagctgc cgttctcgtc
     2761 tcccgacgat gcgctgacca aggcgcgcat cgagcacctg aaagccactg gcggcaacgc
     2821 attccgcgag taccagctgc cggaagcggc gctggcgctc aagcagggcg taggccggtt
     2881 gattcgcagc gagacggatc gcggcgtggt ggtcatctgc gatccgaggc tcatcgacaa
     2941 accctatggg cgggtgtttc gcgccagcct gccgcccatg ccgatcacgc gcgcgggtgc
     3001 cgacgccgaa aaattcctgc ggcgcatcgc cggaactgcg gcggtgccca cggggctcac
     3061 gcgaattccc gaggccgggg aaatgagcga cgaaatcgca tgaagatcct cgccatcgac
     3121 acggccaccg agggatgttc ggtgtcgctc ggcatcggtg atcgctccat cgaccgctac
     3181 gttgaactcg aacgcggtca cgcggagcaa ctgctcccca tggtcgattc cgtgctggcc
     3241 gagggcggca ttgccctcaa tgcgctcgat gcaattgcct tcggacgggg gccgggtgga
     3301 ttcacggggg tgcgtctcgc cgcgagcgtc gcgcaggggc tcgcatttgg tgcgcaggtg
     3361 ggtgtggtgc ccatctccga cctggctgcc gtggcacagc gggtcgttca gctggattcg
     3421 tccgtaaagc gcgtgctcgt ggccaacgac gcgcgcatga aggaagtcta ttgggcgacg
     3481 ttcgaggtcg atggtgtgct cacgtcgggc gcggaacacg tgagcgccgc ggcggcggtg
     3541 ctcctgccgc cggacacgag cggggcctgg gctgccgccg gacgcggcct cctggcctgg
     3601 cccgcgttgg ccgagagctg ccgggtggcg ggcgccagat tgcacgccga cctgctgccg
     3661 cgtgccagcg aggtgctggc tctcgcgcgg ctcgccgtgg cggccggcca aatcctcgat
     3721 ccagccgacg cgttgcccgt ttatgtcagg gaccgtgtag cacaatccgc tttgtaatca
     3781 atctgcaata gactgccgtt gtaatgcgca cgccacaaaa acgtcacttg tgatgccgtg
     3841 tcagcaaccg tgtcggtaac catgtcagcc aaacagattc tggtagtcga ggacgagcgc
     3901 ccgatacgcg agatgattgc gttcggattg cgccgtgccg ggttcgaggt ccgcgaggcg
     3961 gccgacgccc gcacggggcg cgccgaagtc gccaacaagc tgcccgactt gctgctggtc
     4021 gactggatgc ttcccgatac cagcggcctc gagttcacgc gcgcgctcaa gcgcgaccgc
     4081 gaaacgcgcg agctgccggt catcatgctc accgcgcgcg ccgaagaggg tgacaaggtt
     4141 gcggggctcg aaggcggcgc cgacgactac atcaccaaac ccttttcgcc gcgcgagctg
     4201 ctggcgcgta tcaatgccgt gctgagacgc accactccgg tcgaaggcac cgagcgcatc
     4261 gatgtcgagg ggctggtcct cgatcagagc agccagcgcg tgagttcggg cgagcgtacc
     4321 gtggcgctgg gacccaccga ataccgcatg ctggcgtttt tcatgacgca ccccgaacgt
     4381 gtttacacgc gcgagcagct tctcgaccgc gtctggggcg gcaatgtcta cgtcgaagag
     4441 cgcaccatcg acgtccacat ccggctgctg cgcaaggccc tcgaggagtt cggttacgac
     4501 cgcctcatcc agaccgtgcg cggttccggt tatcgcttct cgtcacggcc actctgacca
     4561 caaaatgaac tcgggcagca ccgcattcct ctacgtcgcg ttgcgggtgc tcgcggcggt
     4621 ggcgctgggt gctgttctgg gctggttcgc aggctcggtg tggatcggcg tgagcggagt
     4681 tctctcgttg tatctcgcct ggaatctctg gcagatacgc gccttgcaat tctggctgca
     4741 gcatcgcagc gtggccgatc cgcccgatgc ccccggtctc tggggcgacg tggtcgcgca
     4801 agtggtgcgc ttgcaccggc gcaagcggtt ccacaaggag cgtctcacgc gcctgttccg
     4861 tgaactgcgc cggtccaccg cggccatgcc cgacggcgtg gtgatgctgg atccgatggg
     4921 tgaaatcatc tggttcaatc gcaaggccgg cgaaatgctc ga
//
LOCUS       DASQJS010000106        14411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_998020, whole genome shotgun sequence.
ACCESSION   DASQJS010000106 DASQJS010000000
VERSION     DASQJS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14411
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998020"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..516)
                     /locus_tag="VEW08_04275"
     CDS             complement(154..516)
                     /locus_tag="VEW08_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ39983.1"
                     /translation="MARMIMTVDDSTSMRQMVRATLISAGYDVVEHADGQEALEYARE
                     NPVDLVITDVNMPRMDGITLVSELRQLPAYRLTPVLLLTTESSMDKKMEGKKAGATGW
                     IVKPFNPAQLLATLSRVL"
     gene            complement(530..1639)
                     /locus_tag="VEW08_04280"
     CDS             complement(530..1639)
                     /locus_tag="VEW08_04280"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HYJ39984.1"
                     /translation="MATKKIRVLVVDDSALVRQILVEVLNSAPDVEVVGTASDPFMAR
                     DRIKELNPDVLTLDVEMPRMDGLTFLANLMRLRPMPVVMVSSLTEQGADTTLKALELG
                     AVDFVSKPKIDLAGTLADFGGEILAKIRIAAGARVMARSTPVAAARIAPKYSADAVIP
                     AGAGGRMLRTTDRIIAIGASTGGTEAIREVLMGLPADCPAIVIAQHIPEAFSLPFTRR
                     MDSLAAMSVVEPTDGQYIMAGHVYIAPGSKHLLVERDGARYRCRLNDGPPVNRHCPSV
                     DVLFRSVAQNVGPNAVGVILTGMGDDGARGLKEMLDAGARTMAQDEASSVVWGMPGAA
                     VKLGGAQEILPLGRVAENIMRLAQTVGAPLIAAAR"
     gene            complement(1644..2513)
                     /locus_tag="VEW08_04285"
     CDS             complement(1644..2513)
                     /locus_tag="VEW08_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HYJ39985.1"
                     /translation="MQAGDTIEFAIAGANDYVLTDAQFHRIRQLVREHTGIALSEAKR
                     QLVYGRLARRLRALKLESFGEYVELLERGVASELEEFTNAITTNLTSFFREPHHFEYL
                     ASDVLPKIVARDSGVRRARIWCCAASTGEEPYSIAMVLREAQDILNGFDVKLLATDLD
                     SAVLATGAAGIYNAERLKSVSAARISRFFRKGSGEQAGKFRAQDELRNLITFKQLNLM
                     HEWPVRGPFDAIFCRNVIIYFDKETQRALFERMAALQRPGDMLFLGHSESLYRVSDKY
                     ELVGRTIYRRNAN"
     gene            complement(2529..3047)
                     /locus_tag="VEW08_04290"
     CDS             complement(2529..3047)
                     /locus_tag="VEW08_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HYJ39986.1"
                     /translation="MSQAAATAESTATQANAPGSSQYVTFVCAGVEYGVEILRVQEIK
                     GWDGVTRVPYTPPYVLGVMNLRGVIVPVIDLRARFGLESKPFDSSTVVIVVRVDAAVG
                     EKTVGIVVDAVSEVYNVAADAVKPTPDLGAGADAACVSGLTSVDDKMVMLLDMNRLVA
                     SSIDLSASQAVD"
     gene            complement(3073..5559)
                     /locus_tag="VEW08_04295"
     CDS             complement(3073..5559)
                     /locus_tag="VEW08_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYJ39987.1"
                     /translation="MKYSNMKVGTRLGMGFGVLLIACLVVGGIGWLRLSQLDEVVNRI
                     TTENWEKARLTMEMEIRTRDNAFKTGRVLMAGNDIEAVKVLKDEMAANSKLNGAALEQ
                     LEKLVVASEAKMLLAEAADARTRYMDSRAQVTRLAADPKTQEDALDLFRTETSGLLEQ
                     YMTPFRKLTERERADFEKASRDSDATYASARNTIVVASVAALIAGILLAITLARSITG
                     PLRNAVAIAKAVRDGQLDNVIDVTGEDETGELLSSLDAMQNALRERDQRDADYRGQIA
                     AIGKSQTVAEFGMDGRILSANENFLNVMGYSLEEVRGQHHSVLLDPAARQSPEYRVLW
                     DKMARGEFDGGQYRRVSKDGWEVWIQASYNPISDAHGKLFKVVEYATDITEQKLRTAD
                     YEGQLAAIGKAQAVIEFNVDGTIRSVNDNFSMMFGYTNADVQGKHHSCLVDPEESAGA
                     EYRAFWVNLARGEAVSSRCKRIARDGREVWVQASYNPIHDANGRPFKVVMYATDVTSQ
                     MLMAHQLEIAVKQTQLTVKAAIDGDLSARIPIGGKSGEIEALCRGVNAMLDNTVELVR
                     RVKTAAAEVQTGAEEISKGNLNLSQRTEEQASSLEETASSMEEMTSTVRQTADNAGQA
                     NQLAMAARQTAEKGGAVVNSAITAMGGINSASKKIADIIGVIDEIAFQTNLLALNAAV
                     EAARAGEQGRGFAVVATEVRNLAGRSATAAKEIKALIQDSVARVDAGSKLVDESGRTL
                     DEIVSAVKKVTDIVAEIAAASREQSSGIEQVNKAVMQMDQTTQQNAALVEEASAASQA
                     IVEQAQALNGMIARYKVDDAGDRASKRRAA"
     gene            complement(5556..6842)
                     /locus_tag="VEW08_04300"
     CDS             complement(5556..6842)
                     /locus_tag="VEW08_04300"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HYJ39988.1"
                     /translation="MNWSVEERRNCRRVVLAVSLLALAAFGRAARAQDADESAGLAVS
                     VSYTGDLRRNTTGGIAVGSAYSDSFDLGATWSSSSLFTGTGFTTSLSVMHLGGDDISG
                     EFVGDMHGINNIEAPDAWHLYEFWSEFNFGGRANTSLRMGVLDLNADFDTPVTSGLFV
                     GSPHGIGTELSQTGGNGPAIWPITGLGIRLAGQFSEGLNWRVGAFEGTPGNDDERSFA
                     TFDVASGEGSLLIGEVEYMSDRVNKISLGMWSYTAEFERLDAELTTGAPSQDGNQGVY
                     ALVDLPLANVGPARIDGSLRLGLADGRFNPVDECVGATIVVSHPFANRPDDAFGLAVA
                     YGRTGNIYRTVQALAGIPAASAETSYELTYRAPLTGWLALAPSVQFVSNPGADLAMRD
                     AWVVGLRFELAHEKSWPMFAQRIAPSSPSVARSGTE"
     gene            complement(6843..9461)
                     /locus_tag="VEW08_04305"
     CDS             complement(6843..9461)
                     /locus_tag="VEW08_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYJ39989.1"
                     /translation="MTLKNFKVGTRLGFGFLLLLLATLVVGGVGLMRLSQLDQMVGQI
                     TDVDWNKARLSMETEARNRENAAKFSRLLLVDSGSDKAASLKAKIASNSEKNAAAIKE
                     LETLLSSPEEKALLEEAAAARETYNASRAEVMGLVADPKTRAAGLEQYNTVTADLIEP
                     YIEALHKLSEMQKETFEKASDTSGAAYEHSRIVILSIIGAAVIFGIVLAILLARSIVR
                     PLGNAVSVAESIKDGHLDNSIDASGEDETSKLLGSLDVMQSALRARDEKDADSRGQIA
                     AIGTSQAVIAFDMEGKILDANENFLRTIGYTLNEIKGQHHSMFVDARERGSSEYRTFW
                     DKLNRGEYDAGQYKRIGKGGKEVWIQASYNPIKDVNGKNIKVVKYATDITEQKMRNAD
                     FEGQLAAIGKSQAVIEFELDGTIRSVNDNFASLMGYSNNEARGKHHNLLVDAATSSSA
                     DYRAFWAKLGRGEPEAGRFKRFAKGGREVWLQASYNPILDANGRPFKVVKYATDVTAQ
                     ILMSQQLELAVAQTQTVVKAAIDGDLTARVPTTGKSGEIEALCKGINSMLETTTELVK
                     RVKTAAAEVQTGAEEISKGNLNLSQRTEEQASSLEETASSMEEMTSTVKQTADNAGQA
                     NQLAMAARQQAEKGGAVVSSAVSAMGGINAASRKIADIIGVIDEIAFQTNLLALNAAV
                     EAARAGEQGRGFAVVATEVRNLAGRSATAAKEIKALIQDSVAKVDEGSKLVDESGRTL
                     DEIVNAVKKVTDIVAEIAAASREQSSGIEQVNKAVMQMDQTTQQNAALVEEASAASQA
                     IVEQAQSLNGMIARYDVGGESAVARPVAKAAPAGADRRTGARPWANRAAPAAAKTAVA
                     APTRKAAGDDAEWKEF"
     gene            complement(9474..9980)
                     /locus_tag="VEW08_04310"
     CDS             complement(9474..9980)
                     /locus_tag="VEW08_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HYJ39990.1"
                     /translation="MSNSSMNTGNASGAIEAADSQQFLTFKLAGEEYGVGILTVQEIR
                     GWSKVTKVPHTPEWLLGVINLRGVVVPIIDLRVKFNFASAEYNDTTVVIILTVASRVV
                     GVVVDGVSDVITLAAAQIKPAPSLGTGANTGHIIGFGTLDERMRILMDVEKLMAGADM
                     GLVDKAIH"
     gene            complement(9977..12031)
                     /locus_tag="VEW08_04315"
     CDS             complement(9977..12031)
                     /locus_tag="VEW08_04315"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HYJ39991.1"
                     /translation="MTLDLTQFHEIFFEESFEALDSMEAALLKLSAGEVDAELINTIF
                     RVAHSIKGGAATFAFNDVAGFTHVLETLLDQLRAGKRQVRPEIVDVLLRSGDAMRAML
                     SATQRKEAIDSAGVAKLRAELDRIMSESGTAAAPAAAASAPSPGKVAASGDKPLPGWR
                     IKFVPGPELLRHGNDPLRILRELATLGNLKVSVDAGRIPALNDLDPEVCCMRWNLEVQ
                     GEIDRAAVLSVFEWAETECELTIERVGGEPSAPAAPIVATAAAAAAPAAPVPAPAAEA
                     STRSANPDGGSIRVSIEKIDELLNSVGELVITQSVLSQLAAPLEGREAGELRSALAQL
                     ERHMRALQESVMRVRMLPISFVFNRFPRLVRDLGQRLGEKIELKVTGDTTELDKTVLE
                     KIGDPLVHLVRNSIDHGIEMPDVRVAAGKVEHGTISLHAYHKGGNVIVEVSDDGGGLR
                     KDKILAKALERGLVTNEAELSEERILNLIFAPGFSTADVVSDVSGRGVGMDVVRRNIN
                     ELGGHIQIFSTQGQGSTIRIRLPLTLAILDGQLARVGKEVYVVPIVSIVETIQVTRER
                     VNAVASGGQVFRLRDDYLPIVRLHSLFDIEPDYTDLLDGLLMIVEADGKRVGLFVDEL
                     MSQQQVVIKSLETNFKQVTGLAGATMLGDGRVALILDIPGVITRFQAAPEERGARKPQ
                     AA"
     gene            complement(12028..12348)
                     /locus_tag="VEW08_04320"
     CDS             complement(12028..12348)
                     /locus_tag="VEW08_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYJ39992.1"
                     /translation="MPAAAVPAPASEARPRPGRVVLGTACTIQEAAAVRAHMLAQAAL
                     PGPYEIDGGSVEMVDTAGVQLVVAFALDCLEKGVPYKWSGRSATLEKAIELLGVGPLL
                     EYPA"
     gene            complement(12571..13050)
                     /locus_tag="VEW08_04325"
     CDS             complement(12571..13050)
                     /locus_tag="VEW08_04325"
                     /inference="COORDINATES: protein motif:HMM:NF022428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HYJ39993.1"
                     /translation="MEFASDWSIYGLRTLPLLGAFAVFAWALMSARRQAAQAHAQVAA
                     RLETALGEIRRLSGQVIALGGSIEALGTRLTEAQAHAEAQARAQAQVPTPPARPAPSA
                     ASNTRGYETAIRMARGGSSVEEIVASCGTTRAEAKLLRRLHECGEPRRAEPQGMRTA"
     gene            complement(13146..14144)
                     /gene="motD"
                     /locus_tag="VEW08_04330"
     CDS             complement(13146..14144)
                     /gene="motD"
                     /locus_tag="VEW08_04330"
                     /inference="COORDINATES: protein motif:HMM:NF006541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HYJ39994.1"
                     /translation="MARKHKHEEHANHEAWAIPYGDLVTLLLAFFVVMYALSTINEGK
                     FRVLSDSLQAAFRGAPRTLEPVNVGEKSRGSGADIAVSIVQQATLNGQPRQMLEAITV
                     DSQIATGGRKNPDKLDSDGKVIVPPALGRVADEVEKALAPLVAADMIAVRRHSTWVEV
                     EIRTDILFASGVAKLSSAALPPLTILAETLAKYPNPVRVEGHTDNRPISTSAYPSNWE
                     LSAARAASVVHLFARGGVDPARLAVIGLGEFRPAQSNDTIEGRNANRRVLIVILAGDQ
                     APEDAFGEDRGVASEPVQPGEPAQPEPPATPIAQPAAPATATPVAAARVDSFQPSN"
     gene            complement(14192..>14411)
                     /locus_tag="VEW08_04335"
     CDS             complement(14192..>14411)
                     /locus_tag="VEW08_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYJ39995.1"
                     /translation="PSKLGQGIAAAFTATIYGIGLANLFFLPIAGKLKGIVAEQTQVR
                     EMIIEGIVSIVLGENPRAIESKLHGFLH"
BASE COUNT         2347 a         4685 c         4570 g         2809 t
ORIGIN      
        1 gcggagttcc gcacccagtg tcaccaacgc ggcgatcgag cccgcaagcg tctccacctg
       61 cgcgtcggtc gtgtgcgccg ccacctccag ctggctcgat gccagctcga gcaaggcgga
      121 aaccggtgtg tttgccagat cgctcatcgt gctttagagc acccgcgaga gcgtcgccag
      181 caactgcgct ggattgaagg gcttgacgat ccagccggtg gcgccggcct tcttgccttc
      241 catcttcttg tccatgctcg actcggtggt cagcagcaac accggcgtca gccggtaggc
      301 aggcagctga cgcagctcgc tcaccagcgt gatgccgtcc atgcgcggca tgttcacgtc
      361 ggtgatcacc aggtccaccg ggttttcacg ggcgtactcc agcgcttcct ggccgtccgc
      421 gtgttcgacg acgtcgtatc ccgccgatat cagcgtggcc ctcaccatct gccgcatcga
      481 ggtcgagtcg tccaccgtca ttatcattct tgccatttct tctctccttc taacgggctg
      541 ccgcaatcag cggcgccccc actgtctgtg ccaatcgcat gatgttttcc gccacgcggc
      601 cgagcggcag gatttcctgc gctccaccca gcttcaccgc ggcgccgggc attccccaca
      661 ccacactcga agcctcgtcc tgcgccatgg tgcgggcacc ggcgtcgagc atctccttca
      721 gtccgcgcgc gccatcgtcg cccatgccgg tgaggatcac gcccacggcg ttggggccca
      781 cgttctgcgc cacggaacgg aacagcacgt cgacgctcgg gcagtgccgg ttgacgggcg
      841 ggccgtcgtt gagacggcag cgataccgcg cgccatcacg ctccactaac aaatgtttgc
      901 tgcccggcgc gatgtagacg tgccccgcca tgatgtattg accatccgtg ggctccacga
      961 ccgacatcgc ggccagtgaa tccatgcgcc gggtgaacgg caggctgaaa gcttcgggaa
     1021 tgtgctgggc gatcacgatc gcgggacaat cggccggcaa ccccatgaga acttcccgga
     1081 tcgcctccgt tccaccggtg gacgcaccga tggcgatgat gcggtcggtg gtacgcagca
     1141 tcctgccgcc cgcaccggcg gggatcacgg cgtcggcgga atacttcggc gcgatgcgcg
     1201 cagcggcgac gggcgtgctg cgcgccatca cgcgcgcacc ggcggcaatg cggatcttgg
     1261 cgagaatttc gccgccgaaa tccgcgagcg ttccggccag gtcgatcttc ggcttcgaaa
     1321 cgaagtcgac cgcaccgagc tcgagcgctt tcagcgtcgt atcggcaccc tgctccgtca
     1381 acgacgacac catgaccacc ggcattggcc gcaggcgcat gaggttggcg aggaatgtca
     1441 gcccgtccat gcgcggcatc tcgacgtcca gagtgagcac gtcggggttg agctccttga
     1501 tccggtcgcg cgccatgaaa ggatcgctgg cggtgcccac cacttcgacg tccggcgcac
     1561 tgttcagaac ctccacgagg atctggcgca ccagcgcgga atcatcgacc accagcactc
     1621 tgattttctt ggttgccata ggtttagtta gcgtttcggc ggtagatggt ccttccaacc
     1681 agttcatatt tgtcgctgac gcggtacagg ctttccgaat gacccaggaa cagcatgtcg
     1741 cccgggcgct gcaatgcagc catgcgctcg aacagcgcgc gctgcgtctc cttgtcgaaa
     1801 tagatgatga cgttgcggca gaagatcgcg tcgaacggac cccgcaccgg ccactcgtgc
     1861 atgagattga gctgcttgaa ggtgatcagg ttgcgcagtt cgtcctgcgc gcggaatttt
     1921 ccggcctgct cgccgctgcc cttgcggaag aagcgcgata tccgcgccgc gctgacggat
     1981 tttaaacgct cggcgttgta gatgccggcc gcgccagttg cgagcacggc ggaatccagg
     2041 tccgtcgcca gcagtttgac gtcgaagccg ttcaggatgt cctgcgcttc gcgcagcacc
     2101 atggcgatgg agtatggctc ttcgccggtg gaagccgcgc agcaccagat gcgcgcgcgg
     2161 cgcacgcccg agtccctggc gacaattttc ggcaacacgt cgctcgcgag gtattcgaag
     2221 tgatgcggtt cgcgaaagaa tgaggtgaga ttggtcgtga tcgcgttggt gaattcttcg
     2281 agctcggacg cgacgccgcg ctccagcaac tcgacgtact cgccgaagga ttcgagtttc
     2341 agcgcacgca gtcgacgcgc caggcgcccg tagacgagct gacgcttcgc ttccgacagc
     2401 gcgatgccgg tgtgttcgcg gaccagctgc cggatgcgat ggaactgcgc gtcggtgagc
     2461 acgtaatcgt tggcaccggc aatcgcaaat tcaatggtgt ctccggcttg catgttggag
     2521 tcgcgcgctc agtccacggc ctgggacgcg gagagatcga tgctcgaggc gaccagacgg
     2581 ttcatgtcca gcagcatcac catcttgtca tccaccgacg tgaggccgct gacacaggcg
     2641 gcgtcggctc cggcgcccag atccggcgtc ggcttcactg catcggcggc cacgttgtag
     2701 acctccgaga ccgcgtcgac cacgatgccg accgtcttct cgccgaccgc cgcatcgacg
     2761 cgcaccacga tgaccaccgt cgagctgtcg aagggcttgg attcgaggcc gaagcgcgcg
     2821 cgaaggtcga tcaccgggac gatgacgccg cgcaggttca tgacgccgag cacgtaaggc
     2881 ggtgtgtaag gcacgcgggt caccccgtcc caacccttga tctcctgcac gcgcaggatt
     2941 tcgacaccgt actcgacgcc cgcacataca aacgtcacgt actgggaact gcccggggcg
     3001 ttcgcctgcg tcgccgtgga ttcagcagtg gctgccgctt gactcatctt tcatgctcct
     3061 gttgatcgtt cgtcacgccg cgcgccgctt cgaagcgcgg tcgccagcgt catcgacctt
     3121 gtatcgggcg atcattccgt tgagcgcctg cgcctgttcg acgatggcct ggctcgccgc
     3181 cgaggcttcc tcgaccaacg cggcgttctg ctgagtggtc tgatccatct gcatcacggc
     3241 cttgttgacc tgttcgatgc cgctcgattg ttcgcgtgaa gcggcggcaa tttccgccac
     3301 gatgtccgtg accttcttga ccgcgctgac gatttcatcg agcgtgcggc ccgattcatc
     3361 gacgagcttg ctgcccgcat cgacgcgcgc gacgctgtcc tggatgaggg ccttgatctc
     3421 tttggcggcg gtcgcggagc gtccggcgag attgcgaact tccgtggcca cgaccgcgaa
     3481 accgcggccc tgttcgccgg cgcgcgcggc ttccaccgcg gcgttcagcg ccagcaagtt
     3541 ggtctggaac gcgatctcgt cgatcacgcc gatgatgtcg gcgatcttct tgctcgcgga
     3601 attgatgccg cccatggcgg tgatggcgga attcacgact gcgcctcctt tttccgcggt
     3661 ctggcgcgca gccatcgcga gctgattcgc ctgccccgcg ttgtcggcgg tctgcctgac
     3721 cgtggaagtc atttcctcca tcgatgaggc cgtttcctcg aggctcgaag cctgctcctc
     3781 ggtacgctgc gagagattga ggttgccctt ggaaatctct tccgcgccgg tctgcacttc
     3841 ggcagccgcg gtcttcacgc gccgtacgag ttccacggtg ttgtcgagca ttgcattgac
     3901 gcccctgcac agcgcctcga tctcgccgga cttgccgcca atcgggattc gtgccgaaag
     3961 gtcaccatcg attgccgctt tcaccgtcag ctgcgtctgc ttcaccgcga tttcaagctg
     4021 gtgtgccatg agcatctgcg acgtgacgtc cgtggcatac atgaccacct tgaacgggcg
     4081 gccattggca tcgtgaatgg ggttgtacga agcctgcacc cacacttcgc gaccatcgcg
     4141 ggcgatacgc ttgcagcggc tggacaccgc ctcgccgcgc gcgaggttga cccagaacgc
     4201 gcggtactct gcgccggcgc tttcctccgg atccacgagg caactgtggt gtttgccctg
     4261 cacatcggca ttggtgtagc cgaacatcat cgagaaatta tcgttgaccg agcggatcgt
     4321 cccgtcgacg ttgaactcga tgacggcctg ggccttgccg atggcggcaa gctggccctc
     4381 gtagtccgcg gtacgcagct tctgctcggt gatgtcggtg gcgtattcca ccaccttgaa
     4441 tagtttgccg tgcgcgtccg agatggggtt gtaggacgcc tggatccaga cttcccagcc
     4501 atccttgctc acgcgccggt actggccacc gtcgaattca ccacgggcca tcttgtccca
     4561 caacacgcgg tactcggggc tctggcgcgc cgcggggtcg agcagcacgc tgtggtgctg
     4621 accacggact tcttccagcg aataacccat cacgttgagg aagttctcgt tggcgctgag
     4681 gatgcggccg tccatgccga actcggccac cgtctgggac ttgccgatgg cggcaatctg
     4741 tccgcggtaa tccgcgtcgc gctggtcgcg ctcgcgcaaa gcgttttgca tcgcgtcgag
     4801 cgagctcagc agctcgccgg tttcgtcttc gcccgtcacg tcgatcacgt tgtcgagctg
     4861 accgtcccgg acggccttgg caatggccac ggcattgcgg agcggcccgg tgatgctgcg
     4921 cgccagcgtg atggccagca ggatgccggc gatcagcgcg gcgacgctcg ccaccacgat
     4981 ggtgttgcgt gcgctcgcgt aggtcgcgtc gctgtcgcgg ctggctttct cgaagtccgc
     5041 acgctcacgc tcggtgagct tgcggaacgg tgtcatgtac tgctccagca atcccgaagt
     5101 ctcggtccgg aacaggtcga gcgcgtcctc ctgcgtcttc ggatccgcgg cgagcctggt
     5161 cacttgcgcg cgcgagtcca tgtagcgtgt gcgggcatct gcggcttcgg cgagcagcat
     5221 cttggcttcc gaggccacca ccagcttttc gagttgctcg agcgctgcgc cgttcagctt
     5281 gctgttcgcg gccatttcgt ccttgagcac cttgaccgct tcgatgtcgt ttccagccat
     5341 gagcacacgg ccggtcttga aggcgttgtc gcgcgtgcgg atttccattt ccatggtcag
     5401 ccgcgccttc tcccagttct cggtggtgat gcggttgacg acttcgtcga gctgcgagag
     5461 tcgcagccag ccgatgccgc cgaccacgag gcatgcgatc agcagcacgc cgaagcccat
     5521 gccgaggcgt gtacccacct tcatgttcga atatttcatt ctgttcccga tcgagcgacc
     5581 gacggagagc ttggcgcgat gcgctgtgca aacatcggcc aggatttttc gtgcgcgagt
     5641 tcgaagcgca atccgaccac ccacgcatcg cgcatggcga gatcggcgcc cgggttgctc
     5701 acgaactgca ccgacggtgc gagcgcaagc cagccggtga gcggcgcgcg atacgtcagc
     5761 tcataagaag tttccgcgct ggccgcagga atgccggcga gtgcctggac cgtgcgatag
     5821 atgttgccgg tgcggccgta ggccacggct aaaccgaaag cgtcatcggg ccggttcgcg
     5881 aacgggtggc tgacgacaat agtcgcgccc acacattcat cgaccgggtt gaagcggcca
     5941 tcggccagcc ccaggcgcaa cgaaccatcg atccgcgccg ggcccacgtt ggcgagcggc
     6001 agatcgacga gcgcgtagac gccctggttg ccgtcctgcg atggcgcacc tgtagtcagc
     6061 tccgcgtcca gccgctcgaa ctccgccgtg tacgaccaca tcccgagcga gatcttgttg
     6121 acgcgatcgc tcatgtattc cacttcgccg atgagcaggc tgccttcgcc gctcgccacg
     6181 tcgaaagtcg cgaagctgcg ctcatcgtcg ttgccaggcg tgccttcgaa tgcgccgacc
     6241 cgccagttca agccttcgct gaactgtccg gcgaggcgga tgccaagacc ggttatcggc
     6301 cagatggctg gtccattgcc acccgtctgg ctgagctcgg tgccgatgcc atgcggcgag
     6361 ccgacgaaca ggcccgaggt caccggcgtg tcgaagtcgg cattcaggtc gagcacgccc
     6421 atgcgcagcg acgtgttcgc acggccgcca aagttgaact cgctccagaa ctcatagagg
     6481 tgccaggcat ccggcgcctc gatgttgttg atgccgtgca tatcgccgac gaactcgccg
     6541 gagatgtcgt caccgccaag gtgcatgact gaaagactgg tcgtgaagcc ggtgcctgtg
     6601 aacaggctgc tggagctcca ggtcgcgcca agatcgaagc tgtccgaata cgccgaaccc
     6661 accgcgattc caccggtggt gttgcggcgc aggtcgcccg tgtagctgac gctgactgcg
     6721 aggccggcgc tttcgtcggc atcttgtgca cgtgccgcgc gcccgaacgc cgccagtgca
     6781 agcagcgaga ccgcgagcac cacacggcga caattgcgcc gttcttcgac cgaccagttc
     6841 acctagaact ccttccactc ggcatcgtca ccggcggctt tgcgcgtcgg tgcggcgacc
     6901 gcggttttgg cggcagccgg tgccgcgcgg ttcgcccacg gacgggctcc cgtacggcga
     6961 tccgcgccgg cgggcgctgc cttcgcgacc ggcctggcga cagcgctttc gccacccacg
     7021 tcgtagcggg cgatcattcc attgagcgac tgcgcctgct cgacgatggc ctggctcgcg
     7081 gccgaagcct cttccaccag cgcggcgttc tgctgcgtgg tctggtccat ctgcatcacg
     7141 gccttgttga cctgctcgat gcccgaagac tgctcgcgac tcgccgcggc gatttccgcc
     7201 acgatgtcgg tgaccttctt caccgcgttc acgatttcat cgagcgtgcg gcccgactca
     7261 tcgacgagct tgctgccttc gtcgaccttg gccacgctgt cctggatgag cgccttgatt
     7321 tccttcgcgg cggtcgcgga gcggccggcg aggttgcgca cttccgtggc gacgaccgcg
     7381 aagccgcggc cctgctcacc ggcgcgcgcg gcttccaccg cggcgttgag cgccagaaga
     7441 ttcgtctgga acgcgatctc gtcgatgaca ccgatgatgt cggcgatctt gcggctggcg
     7501 gcattgatgc cacccatggc gctgactgcc gagctcacta ccgcgccacc cttctccgcc
     7561 tgctggcggg cggccatggc gagctgattc gcctggccgg cgttgtcggc ggtctgcttg
     7621 accgtcgagg tcatctcttc catcgatgac gccgtctctt cgagcgacga ggcctgctcc
     7681 tcggtgcgct gcgaaagatt caggttgccc ttggatatct cctcggcgcc ggtctgcact
     7741 tcggctgccg cggtcttcac gcgcttgacc agctcggtgg tggtctcgag catcgagttg
     7801 atgcccttgc acaaggcttc gatctcaccg gacttgcccg tggtggggac gcgcgccgtc
     7861 aggtcgccgt cgatcgcggc cttcaccacc gtctgcgtct gagccaccgc cagctcgagc
     7921 tgctgcgaca tcagtatctg cgcggtcacg tcggtcgcgt acttcacgac cttgaacgga
     7981 cggccattcg catcgaggat ggggttgtac gaggcctgca gccacacttc gcggccgccc
     8041 ttggcgaagc gcttgaagcg cccggcctcg ggttcaccgc ggccgagctt ggcccagaac
     8101 gcgcggtaat ccgcgctgct gctggtggcg gcatccacca gcaggttgtg atgcttgccg
     8161 cgcgcttcgt tgttcgagta acccatcagc gacgcgaagt tgtcgttgac cgaacgaatc
     8221 gtgccgtcga gctcgaattc gatgaccgcc tgcgacttgc cgatggccgc gagctgacct
     8281 tcgaagtccg cattgcgcat cttctgctcg gtgatgtcgg tggcgtactt gacgaccttg
     8341 atgttcttgc cgttgacatc cttgatcggg ttgtaagagg cctggatcca gacttccttt
     8401 ccgcctttgc cgatccgctt gtattgcccg gcgtcatatt cgccgcggtt gagcttgtcc
     8461 cagaacgtgc ggtactccga agacccgcgc tccctggcat cgacgaacat gctgtggtgc
     8521 tggcccttga tctcgttcag cgtatagccg atcgtccgga ggaaattttc attcgcgtcg
     8581 aggatcttgc cttccatgtc gaaggcgatg accgcctggg acgtaccgat ggcggcgatc
     8641 tggccgcgtg agtccgcgtc cttttcatcg cgcgcccgga gcgcactctg catgacgtcc
     8701 agcgaaccga gcagcttgct ggtttcatct tcgcctgagg catcgatcga attgtcgagg
     8761 tggccgtcct tgatggactc ggcgaccgac acggcattgc cgagcggacg gacgatgctg
     8821 cgcgccagca ggatcgccaa cacgataccg aagatcactg cagcgccaat gatcgaaaga
     8881 atgacgattc gcgaatgttc ataggccgcg ccgctggtgt cgctggcctt ctcgaaagtc
     8941 tccttctgca tctccgagag cttgtgcaac gcttcgatgt acggctcgat caggtcagca
     9001 gtcaccgtgt tgtactgttc gagtcctgcc gcacgggtct tcggatccgc gacgaggccc
     9061 atcacttccg ctcgcgacgc attgtatgtt tcacgagccg cggcagcttc ctccagcagt
     9121 gccttctcct cgggcgatga caacagcgtc tcgagttcct tgatggcagc ggcgttcttc
     9181 tcgctgttcg acgcgatctt cgccttcagg gatgcggctt tgtcgcttcc gctgtcgacc
     9241 agcagcaggc gggaaaactt tgccgcgttt tcgcgattgc gcgcttccgt ttccatcgaa
     9301 aggcgcgcct tgttccagtc cacatccgtg atctggccga ccatctgatc gagctgcgac
     9361 aggcgcatga ggccgacacc gcccaccacg agggtggcga gcagcagcag caagaacccg
     9421 aaacccagtc gggtacctac cttgaaattc ttgagagtca ttttcaacag ctcctagtga
     9481 atggctttgt cgaccaggcc catgtcggcc ccggccatca atttttcgac gtccatgagg
     9541 attcgcatgc gctcatcgag cgtgccgaaa ccgatgatgt ggccggtatt cgctcccgta
     9601 cccagcgaag gcgcaggctt gatctgcgcg gcggcgagcg tgatgacgtc ggagacgccg
     9661 tccaccacca cgccgacgac ccggctggcc acggtgagga tgatcaccac cgtcgtatcg
     9721 ttgtattccg cgctcgcgaa attgaacttg acgcgcaggt cgatgatggg cacgaccacg
     9781 ccgcgaagat tgatgacgcc taacaaccac tccggcgtat gcggcacctt ggtgaccttg
     9841 ctccagccgc ggatctcctg cacggtcagg atgccgacgc cgtattcctc accggccagc
     9901 ttgaaggtca ggaattgctg gctgtcggct gcctcgatgg cgccgcttgc gttcccggtg
     9961 ttcatggaag aattgctcat gcggcttgcg gcttcctggc gccgcgctcc tctggtgctg
    10021 cctggaagcg ggtgatgaca cccggaatgt cgagaatcag cgccacgcgg ccatcgccga
    10081 gcatggtggc gcccgcaagt cccgtgacct gcttgaaatt ggtttccagg ctcttgatga
    10141 cgacctgctg ttgcgacatg agctcgtcga cgaacaggcc gacgcgcttg ccgtcggcct
    10201 cgacgatcat gagcagcccg tcgagcaggt cggtgtaatc cggctcgatg tcgaacaggc
    10261 tgtgcagccg cacgatgggc agatagtcgt cgcgcaggcg gaacacctgt ccgccgctcg
    10321 ccacggcgtt gacgcgctcg cgcgtcacct ggatggtctc gacgatcgag acgatgggca
    10381 ccacgtagac ttccttgccg acgcgcgcca gctggccatc gaggatggcg agcgtgagcg
    10441 gcaggcgaat gcgtatggtg ctgccctgtc cctgcgtcga gaaaatctgg atgtgtccgc
    10501 cgagctcgtt gatgttgcgg cggaccacgt ccatgccgac accgcgtccg gacacgtcgc
    10561 tgacgacgtc ggccgtggaa aagccgggag cgaaaatcag gttgaggatg cgctcctcgg
    10621 agagctcggc ttcgttggtg acgaggccgc gctccagcgc cttggcgaga atcttgtcct
    10681 tgcgcaggcc gccaccgtca tcgctgactt cgacgatcac gttgccgccc ttgtgatagg
    10741 catgcaggct gatggtgccg tgctcgacct tgcccgcggc gacgcgtacg tcgggcattt
    10801 caatgccgtg gtcgatggag ttgcgcacca ggtgcaccag cgggtctcct atcttctcga
    10861 gcacggtctt gtcgagctcg gtcgtgtcgc cggtgacttt cagctcaatc ttctcgccga
    10921 ggcgctggcc caggtcgcgc accaggcgcg ggaagcggtt gaacacgaaa ctgatgggca
    10981 acatgcgcac acgcatgacg ctttcctgca gcgcgcgcat atggcgttcg agctgcgcga
    11041 gtgcgctgcg caattcaccc gcctcgcggc cttcgagcgg ggcggcgagc tggctcaata
    11101 cggattgcgt gatcaccagt tcgccgaccg agttcagcaa ctcgtcgatc ttctcgatgc
    11161 tgacgcgaat cgatccgccg tccgggttgg cgctgcgcgt ggaagcttcg gccgcaggtg
    11221 cagggaccgg cgctgcgggc gccgccgcgg ccgcggccgt ggcgacgatg ggagcggccg
    11281 gcgcggaagg ctcgccgccg acacgctcga tggtcagttc acattcggtt tcggcccact
    11341 cgaacacgga gagcacggcg gcacgatcga tctcgccctg gacctcgagg ttccagcgca
    11401 tgcagcagac ttcagggtcc agatcgttga gtgcgggaat gcgcccggca tccaccgaca
    11461 ccttcaggtt tccaagcgtc gccagctcac gcaggatccg cagcggatcg ttgccatggc
    11521 gcagcagttc gggaccggga acaaatttta tacgccagcc cggaagcggc ttgtcgcctg
    11581 acgcggcgac tttgcccggc gacggcgcgg aagccgcggc ggccggcgcg gccgcggtgc
    11641 cgctctcact catgatgcga tcgagctcgg cgcgtagttt ggcaacgccg gcggagtcga
    11701 tcgcttcctt gcgttgcgtg gccgacaaca tggcgcgcat ggcgtcgccc gagcgcagca
    11761 gcacatcgac gatctcggga cgcacctggc gtttgccggc gcgcagctgg tcgagcagcg
    11821 tctcgagcac gtgcgtgaac ccggccacat cgttgaaggc gaacgtcgcg gcgccgccct
    11881 tgatggaatg cgcgacgcgg aaaatcgtgt tgatgagttc ggcgtcgact tcaccggcgg
    11941 acagcttgag cagcgccgcc tccatcgagt cgagtgcctc gaagctttcc tcgaagaaga
    12001 tttcgtggaa ttgcgtgagg tcgagcgtca tgccgggtac tccagcagcg ggccgacgcc
    12061 gagtagttcg atggcctttt cgagcgtggc cgaacgtccc gaccatttgt aaggcacacc
    12121 cttttcgagg cagtcgagtg cgaacgccac cacgagctgc acgccggcgg tatcgaccat
    12181 ttcgacgctg ccgccgtcga tctcgtacgg tccgggcaac gcggcctgcg cgagcatgtg
    12241 ggcgcgcacc gccgccgctt cctggatcgt gcacgcggtg ccgaggacca cgcgtccggg
    12301 gcgaggacgt gcttcggaag cgggagcggg aacggctgcg gcaggcattg ctgcggaagc
    12361 cgttaccgag ggagtcattg tcgcgggagc ctttgtcaca ggtacaacgg caacagcgcg
    12421 agcatggcga ggggccgctg ccttcgagcg tgatttcttg cgcgtgactt tcatggattc
    12481 tgtaaaaccg ggtgaacgcg tatcccgcgc ctgcatgacg ttgtcggcgc aaatggccaa
    12541 gtcgttgagg ggaaacccat cacatccctg tcacgcggtt ctcatgcctt ggggctcggc
    12601 cctacggggt tcgccgcatt cgtgcagtcg ccttagtagt ttggcttctg cgcgcgtcgt
    12661 gccgcagctc gcgacgatct cttcgaccga ggaaccgccg cgtgccatac ggatcgccgt
    12721 ttcgtatccg cgggtattcg acgcggccga aggagccgga cgcgcaggcg gcgtcggtac
    12781 ctgggcctga gccctggcct gggcctcggc gtgggcctgg gcctcggtga gccgcgttcc
    12841 gagagcctcg atcgaaccac ccagcgcgat cacctggcct gacaacctgc ggatctcacc
    12901 gagtgccgtt tccagccgcg ccgctacctg ggcgtgggcc tgtgcggcct ggcgccgcgc
    12961 gctcatcagc gcccaggcaa agaccgcgaa agcgcctaac agcggcagcg tgcgcagacc
    13021 gtagatcgac caatccgatg cgaattccat acacgagctc ctgacgtccg ccgctgggga
    13081 tcacgcgtca aagagtcgac ggcatgaggc ggcaaggagg aacggagcag gaaccgtgcc
    13141 agcgcctaat ttgacggctg gaatgaatcc acgcgggcgg cggccacggg tgttgcggtg
    13201 gccggcgcgg ccggctgtgc gatgggtgtg gcgggaggct ccggctgcgc aggttcgccc
    13261 ggttgcacgg gctcgctcgc cacgccgcgg tcttcaccga atgcgtcttc gggtgcctgg
    13321 tcgccggcga ggatcacgat cagcacgcga cggttcgcgt tgcgcccctc gatcgtgtca
    13381 ttgctttgcg cggggcgaaa ttcaccgagc ccgatgacgg ccagccgcgc cgggtccacg
    13441 cctcccctcg cgaacaggtg cacgacgctc gccgcacgcg ccgcggacaa ctcccagttc
    13501 gacggatatg cggaggtgct gataggacga ttgtccgtgt ggccctccac gcgcaccggg
    13561 ttcggatact tggcaagcgt ctcggcgaga atggtgagcg gcggcaaagc cgcgctggaa
    13621 agcttcgcga cgccgctcgc gaacaggata tcggtacgga tctcgacttc gacccacgtg
    13681 gaatggcggc gcaccgcgat catgtcggcg gccacgagcg gcgccagcgc cttttcgact
    13741 tcgtcggcca cacggccgag cgcgggcgga acgatgacct tgccgtcgga atcgagtttg
    13801 tcggggttct tgcgcccgcc cgtggcgatc tgactgtcga cggtgatcgc ctcgagcatt
    13861 tggcgcggct gcccgttcag ggtcgcctgc tgcacgatcg acacggcaat gtcggcgccc
    13921 gagccgcgcg atttctcgcc cacgttgacc ggctctagcg tccgcggcgc gccgcggaat
    13981 gccgcctgca gcgaatcgga caacacgcgg aacttgccct cgttgatcgt cgagagcgcg
    14041 tacatgacca caaaaaaagc cagcagcagc gtgacgaggt caccgtaggg aatggcccac
    14101 gcctcgtgat tggcgtgttc ttcgtgtttg tgctttctag ccataagaat ggtgccaggc
    14161 accgtttcct agcaatcggt gcctgacacc attaatgcag gaagccatgc agcttggatt
    14221 cgatggcgcg cgggttctcg ccgagcacga tcgacacgat gccttcgatg atcatctcgc
    14281 gcacctgggt ttgctcggcg acgatgccct tcagcttgcc ggcgatcggc aggaagaaca
    14341 ggttcgcgag gccgatgccg tagatggtcg cggtgaacgc cgcggcgatg ccctgcccga
    14401 gcttcgaggg g
//
LOCUS       DASQJS010000107        14357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2884492, whole genome shotgun sequence.
ACCESSION   DASQJS010000107 DASQJS010000000
VERSION     DASQJS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14357
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2884492"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1312)
                     /locus_tag="VEW08_04340"
     CDS             complement(161..1312)
                     /locus_tag="VEW08_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYJ39996.1"
                     /translation="MKKLKLVLLLSVAIVVAVFLFNFGDARRHALVMFAGEDAVAYQE
                     PRIVVAMPVGPPQPKVSPAEAGIAMAGIQAAVDYAGARNTRALVIGHGGHIVFEKYWD
                     GSTLDTPVDLSGFTPVLSALLLGIAMNDERSINLDAPLGGYIAEWADDPRGAISLRQL
                     VTRSSGFAKPGGRPWPGTRASRYALSTDLRPVLLAWPLDAALRQGESPAGLNADILAM
                     ALSARLGQPFDKLLSERLWGPVGAGEFSLARGARAGCCIRARLGDWMRIGEALANNGV
                     FEGNQLTPPRYVSLMLKPAYAGSTMGFFTRVGGTFAARDVAWLESAGKQRLWIVPSLR
                     LTILRVGDEPPESQGWDEAMIPDSIIRNVNGWQPGAVGEGVDPNRFAPH"
     gene            complement(1309..2802)
                     /locus_tag="VEW08_04345"
     CDS             complement(1309..2802)
                     /locus_tag="VEW08_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ39997.1"
                     /translation="MSSSRAVNSLSLTLKRIDLDRGDRAVLRDVNWRIQPGQRWLLIG
                     GNGAGKTQLLKLLAGSVWPKPTGKELRRYRWRGENFDMPAGVSDEIAYVGAERQDRYE
                     HYEWNFRAREVVGTGVQRTDIPMRELTPVEDKHVGALFQRLGIAALADRRFLTLSYGE
                     RRLVLIARALASRPKLLLLDEVANGLDSRNHARFLAWLNGTVRSSMPWVFATHRREDV
                     PASMTHLLELEQGVVKQSGRMRADRARALLQQDASAFHSRHASTKKKPRKRRIVVAMR
                     HADVHLDEAHILTDINLEVRAGDCWVVHGPNGSGKSTLLRTIYGDHAVASGGSIVRAG
                     IVPGVALEKFKLRTAYVAPHLQTWHKPAMPVMEVVASGRYASIGLNDTITAADRKHAE
                     RALRRFGMYSMRGRTLAEMSYGQARRILFARAWAREPTLALLDEPFAGLDRTTRADLT
                     RRLNDWLAEGGSCVIATHHREEWPAHTTHVLEVNDGRVVMTHALGEA"
     gene            complement(2849..3712)
                     /gene="phhA"
                     /locus_tag="VEW08_04350"
     CDS             complement(2849..3712)
                     /gene="phhA"
                     /locus_tag="VEW08_04350"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HYJ39998.1"
                     /translation="MTRLPAAPTQSAVKLRGDYAHAAADFRLPQNYDSYTADEQAIWR
                     TLYERQLSMLGEHAAPEFMAGLRLLGADAHRIPVLEEASERLKRLTGFEVIGVPGLIP
                     EADFFAHLAERRFPVTVWMRRRDELDYLVEPDLFHDFFGHVPMLAHTVFANFIQAYGA
                     IGAHAQSPAALKMLARLYWYTVEFGLVHTSRGLRAYGAGILSSSGETIYATRGTGPQR
                     IAFDLQRVLRTNYLIDSYQKTYFVLDDIEQLFDSVLAADFEMLFRHADEPAYAPDARV
                     PGDQPASLSVS"
     gene            3828..4304
                     /locus_tag="VEW08_04355"
     CDS             3828..4304
                     /locus_tag="VEW08_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HYJ39999.1"
                     /translation="MKLDKTDRRILDVLQRDGRISVTDLAEKVGLSATPCARRIRQLE
                     DSGLIQGYAAIVDPKRAGQTIQAIVQIKLEQHSDEIVERFRRTLIDRPEVLACYTMTG
                     EMDFLLHIIVRDIEALSDFTMRRLLRVSGVRDVTSSIVLETVKRSPVIPLEELAPE"
     gene            complement(4342..5052)
                     /locus_tag="VEW08_04360"
     CDS             complement(4342..5052)
                     /locus_tag="VEW08_04360"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYJ40000.1"
                     /translation="MAPTLSAGASPAQQPAGWRSLSDLTDDRLVALACEHNLLAFEAL
                     MRRHNRRLFRVTRSVLRDSDAAQDAVQETYLRAFTKLDSYTPSGRFGAWLSRVAFNEA
                     LMMRRRVRGDTISLEDAGDDAMAVEEATASDAPSAEQFLEAAHARALLEHAIDALPEN
                     FRTVFVLRAVEGLDVRETAECLGVNAATVRTRLFRAQRLLRVDLSRRLEGESSEIFDF
                     GAERCDGVVAYVLARLPH"
     gene            5120..6229
                     /locus_tag="VEW08_04365"
     CDS             5120..6229
                     /locus_tag="VEW08_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HYJ40001.1"
                     /translation="MKRRYKVLLWAAGTVGVAVIGLRLVLSHDSPCSAAPTLAEGDTP
                     MKGWVHRCYGGPDIVQYEDLPKPVSADDEVIVKVRAASVNPLDWHYMRGKPYVMRLMG
                     SGVGSPKDPLLGVDFAGVVESVGKSVTKFKPGDEVFGGRDGAFSEYVRVRESRNIALK
                     PANISFEEAAAVPIAAITALQAVRDSGKLKAGEKVLVNGASGGVGTFAVQIAKSMGAE
                     VTGVCSGRNVELVRSLGADHVIDYTREDFTAGATKYDLIVDNVVTHSFSDYRRVLTDS
                     GRFVIVGSNNDGDYLGPMSDALKAGIYDPMVSQEFGFMMSSMKPEDLTTLRDMLEAGT
                     IRSIIDRTYRFSEVPEAIRYVETGRARGKVVVKFD"
     gene            complement(6260..7024)
                     /locus_tag="VEW08_04370"
     CDS             complement(6260..7024)
                     /locus_tag="VEW08_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40002.1"
                     /translation="MTCTGEMRWMPKHGRLLQALVLILVVSIGILSTSRAEPIACLAG
                     QKGCWELVIETKVRTSFDRAAYVFVGQLEAVQDSKSGGSDTRIFTFRIGKQYKDSLVG
                     NVARISIDSYLTGGKTAGAHPQKSLDEFEQLEAAAESIDTEEAKNQYEQRVMLLRDEI
                     KKNGSAESAPTHVVHLDLMLGDVPIRNTDIPMRIGERYAVFVLSKAALAPEPDKDTGS
                     LKTWIGDPVDLYSMNDDRGARALAALEQASKSKPLN"
     gene            complement(7120..7196)
                     /locus_tag="VEW08_04375"
     tRNA            complement(7120..7196)
                     /locus_tag="VEW08_04375"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7160..7162),aa:Arg,seq:tcg)
     gene            7278..8024
                     /locus_tag="VEW08_04380"
     CDS             7278..8024
                     /locus_tag="VEW08_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HYJ40003.1"
                     /translation="MAKKFYVVWRGVKTGVFHDWPTTLALVDGFPGAQYKSFPTLAEA
                     EVAFRRGPPARNTATGASKPRIVRTDHADAVAAFDTVIFCDGACEPNPGKAGSGMAVY
                     RAGELASLWYGAYNPAGTNNTAELNALLHALQFARTEIDAKRTVQVRSDSSYGLNAVT
                     KWAAGWKKRGWRKADGEIKNLEVIQELYALYQEIEDDAVLLHVSAHVGIEGNELADRM
                     AMLAVERREVQWREYDGALDIDKLLKMRPG"
     gene            complement(8034..9227)
                     /locus_tag="VEW08_04385"
     CDS             complement(8034..9227)
                     /locus_tag="VEW08_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HYJ40004.1"
                     /translation="MDIARARPFEISVGDPILADLDVRLAATRLPPEQGPEGWDAGAS
                     PAYLRDLLEHWRNHYDWRLEERKLNQFGQFRAEVRGADIHFIHERARNHAGGKPPLPL
                     ILTHGFPDSFTRFLKIIPMLTDPAAHGGQAEDAFDVVVPSLPWCGFSHEHRHRSGLFG
                     VNDIWHALMTEVLGYERFGAHGGDWGSTITEHIARSHSNSVIGIHLTDVPFWHSLKTP
                     ADLRPVEREYVEHIQRFQQEHGAYAMIQGRKPQTLADGLNDSPIGLAAWLTEKFQRWS
                     DCDGDLDACFTRDEMLTNVMVYWVTGSIGRAFMPYFDVLNAGPPRWIAEVVKQKLGSH
                     EVPAGFAMFPRDLSHPPREWAERFFNVQRWTEFPKGGHFAAMEQPDALARDIREFFRP
                     LRSLQ"
     gene            complement(9199..10401)
                     /locus_tag="VEW08_04390"
     CDS             complement(9199..10401)
                     /locus_tag="VEW08_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HYJ40005.1"
                     /translation="MVTLASTQPYRIGISRQVLDDLRVRLHRTRRVRVLDNAGWKGGS
                     DPDYLEALIGYWRDEYDWRRHERELNQFAQFVADIDDARLHYIHEPGLSVRSVPVLLL
                     HGWPDSFLRYRAVIPLLVGAQDDYADDLTCDVVVPSLPGFAFTGQVGPPSEVPSIKTS
                     AWLLHRLMTEVLGYEQFIVAGGDGGSAIAQSMAIQFPASVIGIHLTDIGWHEHGGDLT
                     DIEKKYVAAVQRKFMEDGAYAAVQMSQPRALAASLNDSPAGLASWVVDRFHSWCDSPR
                     HLEQSFSKDEMLTNITIYWVTQTIQSSIYGYIAEARQPSLGKGEHVDCPVALALFPRD
                     MVGALPRRLAERTLNVVRWTEMQSGGHFGAWERPAEFASDLKGFVVSLADPLGQRALD
                     GYRARKAV"
     gene            complement(10513..12501)
                     /locus_tag="VEW08_04395"
     CDS             complement(10513..12501)
                     /locus_tag="VEW08_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ40006.1"
                     /translation="MTMLKPVRALLLLSTTVCSATTYAADPASSIDTVVVSASPITPE
                     DKLATIVESVDRDEILRAGGANLADALANTPGVTGTGFASGSSRPVIRGFDASRVRTL
                     EDGIGSFDVADVGPDHGIPIDPLSAQRIEVIRGAATLRYGSQAIGGVVNAINNRVPRL
                     LPDEPFAAELTGLYGTNADTRQGSALLDGRVGSLAIHADGFKRESHDYDTPDGTMTNS
                     FFRGNGYSLGGSYLFDKNRVGAAVIHYDSEYGIPGEGAFIDMKQTKELLRTSFAPEIG
                     AFHTLTIEGGHADYEHSEIELETGEVGSTFKDDEWDARAEALFGALGPFSAAALGVQF
                     QNRDFSALGEGEDYLLPTETRTAAAFAFADAPLSERLHLHIGARVESVDVDGTPGDDV
                     ATSRSFTPISASAGLLFDATDALRLGFTASSAARAPGQTELYARGPHEATATFETGNP
                     EFDEERANSLEATLRYDTEKSRFEVAVWGSDFTDYIFGNLTGRTCDDEGICVDDDSGE
                     LDELMYEQRDALYWGAEARATIDLIEAAGAPLRGILLADYVRATLDDGGEVPRISPYH
                     VGLGLNWDGQQLGGGFLVKYTGPREDNLATAETPTAGFVSVDAQAMWRPFEKSPDIEI
                     ALVGRNLTDRLQRNAVSLNKDEVILPGRDVRLMVRAKF"
     gene            complement(12567..12920)
                     /locus_tag="VEW08_04400"
     CDS             complement(12567..12920)
                     /locus_tag="VEW08_04400"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HYJ40007.1"
                     /translation="MSITALAGILTRGGPLIRRNRRWFVHVLSLAMLVAQLGMVVHAS
                     SHLKSDPHATPTPAQLCGQCASFAPLQNMVGGAPTVLLAVHVSHERALEFAAVVSVPQ
                     RAFTAFRSRAPPNPS"
     gene            complement(13031..>14357)
                     /locus_tag="VEW08_04405"
     CDS             complement(13031..>14357)
                     /locus_tag="VEW08_04405"
                     /inference="COORDINATES: protein motif:HMM:NF004784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HYJ40008.1"
                     /translation="MGAASCDLTTIGISGGPMLSGRFRGKQIGSGTGVWQMSEDVRAG
                     KMSQEEFTAAESCMHRSKGHCMTMGTASTMASMVEALGVSLPGNAAIPAVDSRRYRLA
                     QLSGRRIVEMVKEDLLLSKILTREAFENAIKANAAIGGSTNSVVHLLAIAGRLGVKLE
                     LEDWDRHGSTLPCLVNLQPSGQYLMEDFYYAGGLPAVLKEIEGVLNKSAMTANGKTMG
                     ENIANAPVWNRDVIKAFDDPFKANAGIAVLRGNLAPDGAVIKPSAATPKLLRHRGPAV
                     VFETIEEFKSKIDDESLDIDENSIMVLKGCGPKGYPGMAEVGNMALPPKVLRKGITDM
                     VRISDARMSGTAYGTVVLHVAPEAAAGGPLALVRTGDIIELDVPARKLHLDVSEAELA
                     RRRAEWKAPEPPKRGYYKLYVEHVQQADKGADLDFLVGGSGTTVTRDSH"
BASE COUNT         2683 a         4521 c         4518 g         2635 t
ORIGIN      
        1 ggcgccaccg cgcaattcac cggcgcccag ggtcaccacg tgcaccgcgc cggacgcgtc
       61 gacgtggaac tggaattcat cgaagggaaa ctggacggcg tgtggatcgt cggccaggcg
      121 gtctccatct tcgaaaccga gatgtccttc gactgaatgg ctagtgaggt gcgaacctgt
      181 tcgggtccac gccctcgccc accgcgcccg gctgccagcc attcacattg cggatgatgc
      241 tgtcggggat catcgcttca tcccagccct gcgattccgg cggttcgtct cccacgcgca
      301 gaattgtcag gcgcagcgac ggcactatcc acaaccgctg cttgccggcg ctttcgagcc
      361 acgcgacatc gcgcgcggcg aacgtgccgc cgacgcgcgt gaagaagccc atcgttgaac
      421 ccgcgtaggc gggcttcagc atgaggctga cgtagcgcgg cggcgtcaat tgattgccct
      481 cgaaaacccc gttgttggcg agagcctcgc cgatgcgcat ccagtcaccg agccgggcgc
      541 gaatgcagca acccgcacgc gcgccgcgcg ccagcgagaa ttcgccggcg ccgaccgggc
      601 cccagaggcg ctcggaaagt agtttgtcga atggctgccc gagcctggcc gagagcgcca
      661 tcgcgaggat gtcggcattg agtccggcgg gcgattcgcc ctgcctgagc gcggcatcca
      721 gaggccaggc gagcagcacc ggacgcagat ctgtgctcaa cgcgtaacgc gacgcacgcg
      781 tcccgggcca gggcctgccg ccgggcttcg caaaaccgct ggagcgcgtg accaactgac
      841 gcaggctgat cgcaccccgc ggatcgtctg cccattcggc gatgtatccg ccgagcggcg
      901 catccaggtt gatcgatcgc tcgtcgttca tcgctatgcc gagcaagagc gcgctgagca
      961 ccggggtgaa tccggacaga tccacggggg tatcgagcgt gcttccgtcc cagtacttct
     1021 cgaacacgat gtgcccgccg tggccaatga ccagtgcgcg cgtgttgcgc gcacccgcgt
     1081 aatcgacggc ggcctggatg ccggccatgg cgatgcccgc ctcggcgggc gacaccttgg
     1141 gttgcggcgg acccaccggc attgccacca cgatgcgcgg ctcctggtac gccaccgcgt
     1201 cctcgcccgc aaacatcacc agggcgtgcc ggcgagcatc gccaaagttg aagaggaata
     1261 cggccacgac aatcgcgacc gacagcagca gcaccagctt caattttttc atgcctcacc
     1321 cagtgcgtgt gtcatgacca cgcgcccatc attgacttcc agtacgtgcg tggtgtgcgc
     1381 gggccactcc tcgcgatgat gcgtggcgat gacacagctg ccgccctcgg ccaaccagtc
     1441 gttgagtcgc cgtgtcaggt ccgcgcgcgt cgtccggtcc agccccgcaa acggttcgtc
     1501 gagcagtgcc agcgtcggtt cacgcgccca ggcgcgcgca aacaatattc gccgcgcctg
     1561 gccataggac atctcggcca gcgtgcgacc acgcatggaa tacattccga aacggcgcaa
     1621 ggcgcgctcg gcgtgtttgc ggtcggcggc cgtgatggtg tcattgagac cgatgctcgc
     1681 gtagcgtccc gacgccacca cttccatcac cggcatcgcc ggcttgtgcc aggtttgcag
     1741 gtgcggagcc acgtacgcgg tgcgcagctt gaatttctcg agcgccacgc cgggtacgat
     1801 gccggcgcgt acgatgctgc cgcccgacgc cacggcgtga tcaccgtaga tagtccgcag
     1861 cagcgtggac ttgccggagc cattcggccc gtgcaccacc cagcagtctc cggcgcgcac
     1921 ttcgagattg atgtccgtga ggatgtgcgc ttcgtccagg tgaacgtcag catggcgcat
     1981 ggcgacgaca atgcggcgct tgcgcggctt tttcttcgtg ctcgcgtggc gtgagtggaa
     2041 tgcgctcgcg tcctgctgca ataacgcgcg agcccggtcg gctcgcattc tgccgctttg
     2101 tttcacgacg ccctgctcca gctccagcag atgggtcatc gaggcgggca cgtcttcccg
     2161 gcggtgcgtg gcgaacaccc agggcatcga tgagcgcacg gtgccgttga gccaggccag
     2221 gaatcgcgcg tggttgcgcg aatcgagccc gttggccact tcgtcgagca gcagcagctt
     2281 cggccggctg gcgagtgcgc gcgcgatgag cacgagccgc cgctcgccat aagaaagagt
     2341 gagaaaccgc cggtccgcga gcgcggctat tcccagccgc tggaataacg cgcccacatg
     2401 cttgtcttcg accggcgtga gctcgcgcat cggaatgtcg gtgcgctgta cgccggtgcc
     2461 caccacttca cgcgcgcgaa aattccattc gtagtgctcg taacgatcct ggcgctcagc
     2521 gccgacgtag gcaatttcgt cgctcacgcc ggcgggcatg tcgaaattct cgccgcgcca
     2581 tcggtatcgt cgcagctcct tgcccgtggg tttgggccac acactgcccg cgagcagctt
     2641 cagcaattgg gtcttgcccg cgccgttgcc gccgatgagc agccagcgct gccctggctg
     2701 gatacgccag ttcacatcgc gcagcaccgc acgatcgccg cgatcgaggt cgatgcgttt
     2761 gagcgtgagg gagagtgagt ttactgcgcg cgagctcgac atgccggcgc gggagcttac
     2821 cggcgaaacc ccggcggcgc gaggcgcttc aggaaacgct taacgaagcg ggctggtcgc
     2881 ccggcacgcg cgcatcgggt gcgtaggccg gctcgtcggc gtgccggaac agcatttcga
     2941 agtccgcggc cagcacgctg tcgaacaact gctcgatgtc atccagcacg aaataggttt
     3001 tctgatacga atcgatcagg tagttggtgc gcaggacacg ctgcagatcg aaagcgattc
     3061 gctgcgggcc agtgccacga gtggcgtaga tggtctcgcc gctcgaggag aggatgccgg
     3121 cgccgtatgc gcgcaggccg cgcgaagtgt gcaccagccc gaactcgacg gtgtaccagt
     3181 acaaccgggc cagcatcttg agcgccgccg gtgactgcgc gtgggcgccg atggcgccat
     3241 atgcctggat gaagttcgcg aacacggtgt gcgcgagcat gggcacgtgt ccgaagaagt
     3301 catggaacag atcgggctca acgagatagt cgagctcatc gcgccggcgc atccacacgg
     3361 tgaccggaaa tcgccgctcg gccagatggg cgaagaagtc cgcctcggga atgaggccag
     3421 gaacgccgat cacttcaaac ccggtgaggc gcttgagccg ttcgctcgct tcttccagaa
     3481 ccgggatgcg gtgcgcgtcg gcacctaaca atcgcaggcc ggccatgaac tcgggcgccg
     3541 cgtgctcgcc cagcatcgag agctgccgct cgtacaacgt gcgccagatg gcctgttcgt
     3601 cggcggtgta gctgtcgtag ttctggggca gccggaaatc cgccgcggca tgggcgtaat
     3661 cgccgcgcag tttcacggcc gactgggtcg gcgcagcagg taatcgagtc atggccgcat
     3721 cttcctcccg cctccttgca ggaatatggc aattttcgtc aggtaatacc gcaaaatatg
     3781 gtgggattca ctacgtatcc gacagtttat gattaaatcc accaattatg aagctcgaca
     3841 agacggatcg acgaatcctg gatgtcctcc agcgggatgg gcggatcagc gtcaccgacc
     3901 ttgccgaaaa ggtcggcctc tcggcgacgc cgtgcgcgcg gcggatccgg caactcgaag
     3961 attcggggct catccagggg tatgccgcca tcgtcgaccc gaaacgcgcg ggccagacca
     4021 tccaggccat cgtgcagatc aagctcgagc agcactccga tgaaatcgtg gagcgattcc
     4081 ggagaacgct tatcgaccgg cccgaggtgc tcgcctgtta caccatgacg ggagaaatgg
     4141 acttcctgct gcacatcatc gtgcgcgaca tcgaagcgct ctcggacttc accatgcgcc
     4201 ggctgttgcg ggtcagcggc gttcgcgatg tcacatcgtc catcgtgctc gaaaccgtca
     4261 aacgctcacc ggtcatcccg ctggaagaac tggcgccaga gtagaccaaa ggcgtcgttg
     4321 cgccatcgcg gtggacagta gtcagtgcgg gaggcgtgcc aacacgtatg ccaccacgcc
     4381 gtcgcagcgc tcggcgccaa aatcgaaaat ctcggagctt tcgccttcca gccggcgaga
     4441 cagatccacc cgcagcagtc gttgtgcgcg aaacagtcgc gttcgcacgg tggcggcgtt
     4501 gacgccgaga cactccgcgg tctcgcgtac gtccagccct tcgacggcgc gcaacacgaa
     4561 aaccgtgcgg aagttttccg gcagcgcgtc gatggcgtgc tcgagcagcg cgcgcgcatg
     4621 ggccgcttcc aggaattgct ccgccgaggg ggcatcactg gccgtggcct cttcgaccgc
     4681 cattgcgtcg tcgccagcgt cttcgagcga aatcgtgtcg ccgcgaacgc gccggcgcat
     4741 catcaatgcc tcgttgaacg ccacgcgaga aagccaggcg ccaaacctgc cgctgggtgt
     4801 gtagctgtcg agcttggtga aagcgcgcag gtaggtttcc tgcaccgcat cctgcgcggc
     4861 gtcgctgtca cgcaagacac tgcgcgtgac acgaaataat ctgcggttgt gtctgcgcat
     4921 gagggcctcg aacgccagca gattgtgctc acaagcgagc gcgacgagcc ggtcatccgt
     4981 cagatcgctc agggagcgcc acccggcggg ttgctgggca gggctcgcgc ctgcactaag
     5041 tgttggggcc atggcgtaac aatgctccgg agcctgggtc tgttcccgtg cggactcagt
     5101 taggtatata cggaacccca tgaaacgacg ttacaaagtt ctgttgtggg ctgccggcac
     5161 cgtgggcgtg gcggtaatcg gtctgcggct ggtactgagc cacgactcgc cttgcagcgc
     5221 cgctccaaca ctcgccgagg gcgatacccc catgaagggc tgggtgcacc gatgctacgg
     5281 cggcccggac atcgtgcaat acgaagattt acccaaacct gtgtcagcgg acgatgaagt
     5341 catcgtgaaa gttcgcgccg cctccgtgaa cccgctggac tggcactaca tgcgcggaaa
     5401 accgtacgtc atgcgcttga tgggctcggg agtgggtagt cccaaagatc cattgctcgg
     5461 cgtggacttc gccggggtcg tggagtccgt cgggaaaagc gtcacgaaat tcaaacccgg
     5521 cgacgaagtg ttcggcggac gtgacggcgc gttctccgag tacgttcgcg tacgcgagag
     5581 ccgcaatatc gcgctgaagc ccgccaacat ttcattcgaa gaggccgcag cggtcccgat
     5641 cgcggcaatc accgccctgc aggcggtgcg cgattccgga aagctcaagg ccggcgagaa
     5701 agtcctggtg aacggcgcat ctggcggtgt cggcactttc gccgtgcaga tcgccaagtc
     5761 gatgggcgcc gaggtcaccg gcgtgtgcag cggccgcaat gtcgagctgg tgcgctcact
     5821 cggtgccgat cacgtcatcg actacaccag ggaagacttc acggcaggcg cgacgaagta
     5881 cgacttgatc gtcgacaacg tcgtcacgca ttcgttttcc gactatcgcc gcgtgctcac
     5941 tgattccggg cgctttgtca tcgttggttc caacaacgac ggtgattacc tgggccccat
     6001 gtccgatgcg ctcaaggccg gcatctatga cccgatggtg agccaggaat tcgggttcat
     6061 gatgtcgtcg atgaagccgg aagatctaac tacactgcgc gacatgctgg aggccgggac
     6121 gatccgctcg ataatcgatc gcacgtacag attcagcgaa gtgccggagg cgatccggta
     6181 cgttgagacg gggcgggctc gcggaaaggt cgtcgtcaaa ttcgactgac ctctgggata
     6241 ttgcgcgccc ggacagaacc taattcaaag gcttcgattt gctcgcctgc tccaatgcgg
     6301 ccagagctcg cgctcctcga tcatcgttca tcgaatacag atcgacgggg tccccaatcc
     6361 aggttttgag actgcccgta tccttgtctg gctccggagc tagagcggcc ttggagagca
     6421 cgaacacggc gtaacgctcg ccgatgcgca tgggaatgtc agtgttgcga ataggcacgt
     6481 cgccgagcat gagatccaga tgcaccacgt gcgttggcgc ggattcggcg gaaccgtttt
     6541 tcttgatctc gtcacgcagc aacatcacgc gttgttcata ctggttcttg gcttcttcgg
     6601 tgtcgattga ttccgccgcc gcctcaagct gctcgaattc gtcgaggctt ttctgcgggt
     6661 gcgcgccggc cgtctttccg cccgtcagat agctgtcaat cgagatgcgc gcaacatttc
     6721 cgactagaga gtccttgtac tgcttcccga tcctgaacgt gaagatgcgg gtatcgctgc
     6781 ctccagactt ggaatcctga acagcctcga gctgaccaac gaacacgtag gcggctcgat
     6841 cgaaagacgt tcgcaccttc gtctcgatga ccaactccca acatcctttc tggccggcca
     6901 gacaggcgat cggttcggcg cggctagtgg agagaattcc gatcgacacc accaggatca
     6961 gtacaagtgc ttgcaacagc cgaccatgtt tgggcatcca gcgcatttcg ccagtgcaag
     7021 tcattcgtca cctcttcact cgtgctgttg ttatagcgga caccgtattg aaaaggccgg
     7081 ttgatccgac gacggatgac cggcctttta atttcaatct ggcgcgcccg gacggattcg
     7141 aaccgccgac cacctggttc gaagccaggt gctctatcca gctgagctac gggcgcgtgg
     7201 gcgggcattg tagccgtact ggctgtcccg gtggttctcc ttggcgcggg gcgcttttgt
     7261 cggcaggatg cgctccgatg gcaaaaaagt tctatgtcgt gtggcgaggc gtcaagaccg
     7321 gcgtattcca tgactggccc accacgctgg cgctggtcga cggttttccc ggcgctcaat
     7381 acaaatcgtt tccgacgctt gcggaagcag aagtggcatt tcgccgcggc ccacccgcac
     7441 gcaacaccgc cacgggagcc agcaaaccac gcattgtgcg cacagaccac gcggacgccg
     7501 tcgcggcatt cgacacggtc atcttctgcg acggcgcctg cgaaccgaat ccgggaaaag
     7561 cgggttcggg catggcggtt tatcgcgccg gcgagctcgc aagcctctgg tacggcgcct
     7621 acaaccccgc cggaacgaac aacaccgccg agctcaatgc actgctgcac gcgttgcagt
     7681 tcgccaggac cgaaatcgat gcgaagagga ccgtgcaggt gcgcagcgat tcatcgtatg
     7741 gactgaacgc cgtgacgaaa tgggctgcgg gctggaagaa acgcggctgg cgcaaggcgg
     7801 acggagagat caagaacctc gaagtcatcc aggaactgta tgcgctctat caggagatcg
     7861 aggacgacgc cgttctcctg cacgtgagcg cgcacgttgg catcgaaggc aatgagctcg
     7921 cggatcgaat ggcgatgctc gcggtcgaac gtcgcgaggt gcaatggcgt gaatacgacg
     7981 gtgcgctgga tatcgacaaa ctgttgaaaa tgcgtccggg ctgaatcgcg aactcattgc
     8041 aggctgcgca gcgggcggaa gaattcccga atgtcacgcg ccagcgcatc cggctgctcc
     8101 atcgccgcga agtgaccgcc cttggggaac tcagtccaac gctgcacatt gaagaatcgc
     8161 tctgcccact cgcgaggcgg gtgcgagaga tccctcggga acatcgcgaa gcctgccggc
     8221 acctcatgcg acccgagttt ttgtttgacc acctcggcga tccagcgcgg ggggccagcg
     8281 ttcagcacgt cgaagtaagg catgaatgca cggccgatgc tgcccgtgac ccaatacacc
     8341 atcacgttgg tgagcatctc gtcgcgtgta aagcaggcat cgagatcgcc atcgcagtcg
     8401 ctccagcgct ggaatttttc ggtgagccac gccgcgagcc cgatcgggga atcattgagt
     8461 ccgtcggcca gggtctgcgg cttgcggccc tggatcatgg cgtaggcgcc gtgctcctgc
     8521 tggaaccgct ggatgtgctc cacgtattca cgttcgaccg gccgcaaatc ggctggcgtc
     8581 ttcaacgaat gccagaacgg cacatcggtg aggtgaattc cgatcactga attggaatgg
     8641 ctgcgggcga tgtgttcggt gatggtgctg ccccaatcgc cgccgtgtgc accgaagcgt
     8701 tcgtaaccca acacctctgt catgagcgcg tgccagatgt cgttgacgcc gaacagtccg
     8761 ctcctgtggc gatgttcgtg cgaaaacccg caccacggga gactcggaac gacgacgtcg
     8821 aacgcgtctt cggcctgtcc gccatgcgcg gccgggtccg tgagcatcgg aatgatcttg
     8881 aggaaccgag tgaaggagtc cggaaacccg tgcgtgagaa tcagcggcag cggcggcttg
     8941 cctcccgcat ggttccgggc acgctcgtga atgaaatgaa tatcggcgcc gcgaacctct
     9001 gcgcgaaact gtccaaattg attcagcttc ctctcttcga ggcgccagtc gtagtggttg
     9061 cgccaatgtt cgagcaggtc acgtagatag gcggggctgg cgccggcgtc ccatccttcg
     9121 ggcccctgct caggcggcaa tcgagtggcc gccaggcgca cgtcgagatc ggcgagaatc
     9181 ggatcgccaa cgctgatttc aaacggcctt gcgcgcgcga tatccatcga gtgcccgctg
     9241 gccgagtgga tcggcaagtg aaaccacgaa ccccttgagg tcggaagcga actccgcggg
     9301 ccgttcccag gcgccgaagt gcccaccgga ttgcatctcg gtccatcgca ccacattgag
     9361 cgtgcgttcg gccaggcggc gcggcagggc gccgaccatg tcccgcggaa acagcgcgag
     9421 cgccaccggg caatccacgt gttcgccctt cccgagcgac ggctgccgcg cttccgcgat
     9481 gtagccgtag atggacgact ggatggtctg tgtgacccag tagatagtga tgttggtcag
     9541 catctcgtcc ttgctgaaac tctgctcgag atgccgcggc gaatcgcacc aggagtgaaa
     9601 gcgatcgacg acccacgagg cgagaccagc cggtgaatcg ttcaggctcg cagccagtgc
     9661 gcgcggctgc gacatttgaa ccgccgcata ggcgccatcc tccatgaatt tcctctggac
     9721 cgcggctacg tattttttct cgatgtccgt gagatccccg ccgtgttcgt gccagccgat
     9781 atcggtgaga tgaatgccga tcaccgaggc gggaaactgg atcgccatgc tctgcgcgat
     9841 agcgctgcca ccgtctccac ccgccacgat gaattgttcg tagccgagca cttcggtcat
     9901 caggcgatgc aggagccagg ccgacgtctt gatcgacggc acttcggatg gcggacccac
     9961 ctgccccgtg aaggcaaaac cgggcagcga cggcacgacc acatcgcagg tgaggtcatc
    10021 cgcatagtca tcctgcgcgc cgacgagcag tggaatcacg gctcgataac gcaggaatga
    10081 atccggccag ccatgcagca acagcacggg caccgatcgg acgctcagcc cgggctcgtg
    10141 aatgtagtgc agcctggcgt catcgatgtc cgccacgaat tgcgcgaact gattgagctc
    10201 acgctcatgc cggcgccagt cgtattcgtc gcgccagtag ccgatcaggg cttcgaggta
    10261 gtcgggatcg ctgccgcctt tccatcctgc gttgtcgagc acccgcaccc ggcgcgtgcg
    10321 atgcagacgg acccgcaggt cgtccagaac ctgcctggag ataccgatgc gatacggctg
    10381 cgtcgatgcc agcgtgacca tgcaaacagc ctactcgcga agtcggtcat ccttctggcg
    10441 cacctgccgt cgttcgagtc tgacgacagc aaaaaaggcg tccctgatga gagacgcctt
    10501 ttgctgcgcc gctcagaact tcgctcgaac catcagccgc acgtcgcggc cgggcaggat
    10561 cacctcgtcc ttgttgagag acactgcgtt gcgctggaga cggtcggtca ggttgcgtcc
    10621 gacgagcgcg atttcgatgt ctgggctctt ctcgaacgga cgccacatcg cctgcgcgtc
    10681 gaccgacacg aagcctgcag ttggcgtttc ggcggtggcc agattgtcct cgcgcggtcc
    10741 ggtgtacttg acgagaaatc caccaccgag ctgctgccca tcccaattga ggccgagccc
    10801 tacgtggtat ggggaaatgc gcggcacctc gccgccgtca tcgagcgttg cgcgcacgta
    10861 gtccgccagc aagatgcctc ggagtggtgc gccagcggcc tcgatcaggt cgatggtcgc
    10921 gcgcgcctca gcgccccaat acagcgcatc gcgctgttcg tacatgagct catcgagttc
    10981 cccgctatcg tcgtcgacgc aaataccctc gtcgtcgcag gttcggccag tcaggttccc
    11041 aaagatgtag tcggtgaaat ccgatcccca tacggccact tcgaatctgg atttctccgt
    11101 gtcatagcgc agcgtcgctt cgagggaatt tgcgcgctcc tcgtcgaact cggggttgcc
    11161 ggtttcgaag gtggcggtcg cttcgtgcgg cccgcgtgcg tacagctcgg tctgccccgg
    11221 cgcgcgcgcg gcgctcgatg cagtgaatcc aagtcgcagc gcatcggtgg cgtcgaacaa
    11281 cagcccggcc gacgcactga tgggggtgaa gctgcgcgat gtcgcgacgt cgtctcccgg
    11341 cgtgccgtct acgtccacag attcgacccg tgcaccgata tgcaaatgca gccgctctga
    11401 cagcggcgca tccgcgaagg cgaacgctgc cgccgtcctg gtttcggtag gaagtagata
    11461 gtcctcgccc tctcccagcg ccgagaaatc ccggttctgg aactgcacac cgagcgcggc
    11521 ggcggaaaat gggcccaggg cgccaaacag ggcctcggcg cgcgcatccc actcgtcatc
    11581 cttgaacgtc gagccaacct ccccggtctc gagttcgatt tcgctgtgtt cgtagtccgc
    11641 gtgaccccct tcgatcgtca acgtgtgaaa cgcaccgatc tcgggcgcga acgacgtgcg
    11701 tagcaactcc ttcgtctgct tcatgtcgat gaaagcgcct tcgccgggaa ttccgtactc
    11761 ggagtcatag tggatcactg cggcgccgac gcggttcttg tcgaagagat aggaaccgcc
    11821 gagggaatag ccattgccgc gaaagaacga attcgtcatc gtgccatcgg gagtatcgta
    11881 gtcatgcgac tcccgcttga aaccatcggc atggatggcg agcgacccca cgcggccgtc
    11941 cagcaacgcg gaaccctggc gcgtgtccgc gttcgtgccg taaagaccgg tgagctccgc
    12001 cgcaaagggt tcgtccggca gaagcctcgg cacgcggttg ttgatggcgt tgacgactcc
    12061 gccgatggcc tggctgccat atcgcagcgt cgccgccccg cgtatgactt cgattcgttg
    12121 tgccgacaaa ggatcgatcg gaattccatg gtccggacca acgtctgcga catcgaagct
    12181 gccgatgccg tcttcgagcg tacgcacccg gctcgcatcg aatccacgaa tgacgggccg
    12241 gctggacccg gacgcgaaac cggtgccggt cacgcccgga gtgttggcga gcgcatcggc
    12301 gagattggcg ccaccggctc gcaggatttc gtcccggtcg acggattcga cgatggtggc
    12361 gagcttgtct tcgggcgtga tcggtgaggc actgacgact accgtatcga tggaagacgc
    12421 ggggtcagcg gcataagtgg tcgcgctgca gacagttgtc gatagcaaca acaaggcccg
    12481 aacgggcttc agcatggtca tggaaaatct ccttccgaaa ctaacaaaca cgcggcgcga
    12541 tacgccggcg tggaatcact tgttgatcag gaaggattgg gtggggcgcg ggaacggaac
    12601 gccgtgaagg cgcgctgagg aacgctgacg accgcggcga attcgagcgc gcgctcgtgg
    12661 gaaacatgga ccgccagcag cacagtgggc gcgccgccca ccatgttctg cagcggtgcg
    12721 aacgaggcgc actggccgca aagctgggcc ggagtgggtg tggcgtgcgg atccgatttg
    12781 aggtggctgc tggcgtgcac caccatgccg agctgggcga ccaacatcgc cagcgacagc
    12841 acatgcacga accaacggcg attgcgtcga atcagggggc ctccgcgcgt gagtattccc
    12901 gcaagagctg ttatcgacaa gctccctcta cggtgccggg ccgggaaacc gggataactt
    12961 ttcgtaattt tgaggagcgt cgcgaggtta cggcgctgga aggttttccc ggtttcccgg
    13021 cccggcaccg ctagtgagag tcgcgcgtca cggtagtacc gctgccgccg acgaggaagt
    13081 cgaggtcagc gcctttgtcg gcctgctgca cgtgctcgac ataaagcttg tagtagccac
    13141 gcttcggcgg ctccggggct ttccactccg cgcgccgcct ggcgagttcg gcctcgctca
    13201 cgtcgagatg cagctttcgc gccgggacat cgagctcgat gatgtcaccg gtcctgacca
    13261 gcgccagcgg cccgccggca gcagcctcgg gcgcgacgtg cagtacgacc gtgccatacg
    13321 cggtaccgct catgcgcgca tcggagatgc gcaccatgtc ggtgatgccc ttgcgcagca
    13381 ccttgggcgg caacgccatg ttgccgacct cggccatgcc gggataaccc ttcggcccgc
    13441 agcccttgag caccatgatg gagttctcgt cgatgtcgag actctcatcg tcgatcttgg
    13501 atttgaactc ttcaatggtc tcgaacacca cggccgggcc gcgatgcctg agtagtttgg
    13561 gcgtggcggc cgaaggcttg atgaccgcgc catcgggcgc gagattgccg cgcaggacgg
    13621 cgatgccggc gttcgccttg aacggatcgt cgaaagcctt gatgacgtcg cgattccaca
    13681 cgggcgcgtt ggcgatgttc tcgcccatgg tcttgccatt ggcggtcatc gcactcttgt
    13741 tgagcacgcc ttcgatttcc ttgagcacgg cgggcaaccc acccgcgtag taaaaatctt
    13801 ccatgaggta ctggcccgat ggctgcaggt tcaccaggca cggcagcgtc gagccgtgac
    13861 gatcccagtc ttcgagctcg agcttgacgc cgagccgccc cgcgatggct aacaagtgca
    13921 ccacggaatt cgtggaacca ccgatggcgg cgttggcctt gatggcgttt tcgaaagcct
    13981 cgcgcgtgag gatcttcgac agcagcagat cttccttcac catctcgacg atgcgccggc
    14041 cgctcaactg cgccagccgg taacgcctcg agtcgacggc gggaatcgcc gcgttgccag
    14101 gcagtgagac gccgagcgcc tcgaccatgc tggccatcgt cgaggcggtg cccatggtca
    14161 tgcagtggcc cttggagcga tgcatgcacg attcggcggc ggtgaattct tcctggctca
    14221 tctttccggc gcgcacgtct tcggacatct gccacacgcc ggttcccgat ccgatctgct
    14281 tgccgcggaa tcgccctgac aacatcggtc cgccggaaat tccgatggtc gtcaagtcgc
    14341 agctcgccgc gcccatg
//
LOCUS       DASQJS010000108         4203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_57995, whole genome shotgun sequence.
ACCESSION   DASQJS010000108 DASQJS010000000
VERSION     DASQJS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57995"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VEW08_04410"
     CDS             complement(<1..542)
                     /locus_tag="VEW08_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYJ40009.1"
                     /translation="MRNVRFLLAPLAAAVLAACAVGPNYQPPETKAAEKFDQLEATYS
                     ADQGNAQFWQTFADSELDKLVSEALASNHDLRIALTRVYEARALRRDAAFDLAPSINV
                     GGGYTKTRSSNAVTPNIARDAELYDAGFDAFWELDFFGGVRRGLEASKADLGAEEAAL
                     QDVQVIVTAEVTRTYFELRG"
     gene            complement(535..3693)
                     /locus_tag="VEW08_04415"
     CDS             complement(535..3693)
                     /locus_tag="VEW08_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HYJ40010.1"
                     /translation="MKFSSYFIDRPVFASVLSAFILIAGAISLFKLPISEYPEVVPPS
                     VVVRAAYPGANPKVISETVAAPLEQEIVGVEGMLYMSSQATIDGSLALTVTFRIGTDI
                     DRAQVQVQNRVAQALPRLPEEVRAIGVSTLKSSPAFLMVVHLNSPDGRYDSLYLRNYA
                     TLNVRDTLARLNGVGDVRVFGAGDYAMRIWLDPQKMSARDLTTGDVVAAIREQNVQVA
                     AGQIGAPPAEGSEFQLALNTQGRLENEEEFGDVVVKTGQNGEVVRLRDISRLELGAAT
                     YATQSLLGNENAVAIPVYQAPGSNALELSNKVRADMETLKKSFPEGMDYKIIYDPTQF
                     VRQSIDAVVETLLEAVLLVVLVVILFLQTWRASIIPLVAVPVSVIGTFAVLYALGFSI
                     NTLSLFGLVLAIGIVVDDSIVVVENVERHIANGLKPLEATKKAMSEVSRPIIAITLVL
                     CAVFIPVAFVEGLTGEFYRQFALTIAISTVISAFNSLTLSPALAALLLKPHGAKPDAL
                     TRGIDKVFGRFFARFNKVFARASDAYSRGATKTVKRAPIAIAVYAGLVALTVLGFYQT
                     PAGFIPQQDKLYLVGVVQLPPAASIDRTNEVVRRMGEIAKQEPGVLDSVQFAGVSANG
                     FAAQSSAALVFFPLKDFDERKGLTAGAIAGALNQKFASIQDAYIAVFPPPPVIGLGTL
                     GGFKLNVEDRQNRGAEALYGAVQTALQKAWADPSLAGVFSSYQINVPQLDVDVDRLKV
                     KRQGVNLNDVFQTLQVNLGSLYVNDFNRFGRTYRVVAQADAPFRSQVDDIASLKTRNA
                     AGEMVPLGSMISVKEAFGPDIVERFNGYSSADINGGPSPGHSSGEAQATISKILDENL
                     PAGMTYEWTDLAFQQIDAGDAALFVFPLCVLFVFLVLAAQYESFSLPLAILLIVPTAL
                     LAGLVGIKLQGGDNNIFTQIGFLVLVGLASKNAILIVEFAKHLQDEGRDRVSAVLEAA
                     RLRLRPIVMTSIAFVMGVLPLVVASGAGAEVRRAMGAAVFSGMIGVTVFGLLLTPVFY
                     VLLRREKTKVEPAQIEEVSAHA"
     gene            complement(3757..>4203)
                     /locus_tag="VEW08_04420"
     CDS             complement(3757..>4203)
                     /locus_tag="VEW08_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ40011.1"
                     /translation="VGSANEQGHPHEGVMVFLDNELDPNTGTIHARGLFKNADRRFTP
                     GMFARVKLIGSGQYDALLINDSAIGTDQSVKFVLRVGAENKIEYTPVKLGPLVDGLRV
                     VREGLKANDVIVVKGLQQVRPGMPVTPQLVAMGEPRRGGQTMVAQN"
BASE COUNT          785 a         1307 c         1361 g          750 t
ORIGIN      
        1 tgcccgcgaa gttcgaagta ggtgcgtgtg acctcagccg tcacgatcac ctgtacgtcc
       61 tgcagggcgg cttcctcggc gccgagatcg gccttgctgg cttcgagacc gcggcgcacg
      121 ccgccgaaga agtcgagttc ccagaatgca tcgaagcccg catcgtagag ttcagcgtcg
      181 cgcgcgatgt tgggagtgac cgcattcgaa gagcgcgtct tggtgtaccc gccgccgaca
      241 ttgatgctgg gggcgaggtc gaacgccgca tcgcgtcgca gtgcccgtgc ctcgtacacg
      301 cgcgtcagcg cgatacgcag gtcgtggttc gacgcgagcg cttcgctcac gagcttgtcg
      361 agctcggagt ctgcgaacgt ctgccagaac tgcgcattgc cctggtccgc ggagtaggtg
      421 gcttcgagct ggtcgaattt ctccgcggcc tttgtctccg gcggctggta gttcgggccc
      481 acggcgcagg cagccaggac tgcggctgcg agcggggcca acaggaaacg gacgttacgc
      541 atgagcggaa acctcttcga tctgcgccgg ttcgaccttc gtcttctccc ggcgcagcaa
      601 cacatagaac acgggcgtca gcagcagacc gaatacggtg acaccgatca ttcccgagaa
      661 caccgccgca cccatggcgc ggcgtacttc ggcgccggcg cccgaggcga cgaccagcgg
      721 caatacgccc atgacgaacg cgatggacgt catgacgatg ggacgcaggc gcaggcgggc
      781 ggcttcgagc accgccgaga cgcgatcgcg gccttcgtcc tgcagatgct tggcgaactc
      841 cacgatcagg atggcgttct tggacgcgag gcccaccagc accaggaacc cgatctgcgt
      901 gaagatgttg ttgtcaccac cctgaagctt gatgcccacg aggccggcca gcagcgccgt
      961 cggcacgatc agcagaatcg cgagtggcaa cgagaagctc tcgtactggg cggcgagcac
     1021 caggaacacg aacaacacgc acagcgggaa gacgaacagg gcggcatcgc ccgcatcgat
     1081 ctgctggaac gcgagatccg tccactcgta ggtcatgccc gcgggcagat tctcgtcgag
     1141 gatcttcgag atggttgcct gtgcttcgcc cgagctgtga cccggcgacg gaccgccgtt
     1201 gatgtcggcg ctcgagtagc cgttgaagcg ctcgacgatg tccggaccga aggcctcctt
     1261 gacggagatc atcgaaccga gtgggaccat ctctccggcg gcgttgcggg ttttaagcga
     1321 agcaatgtca tccacctgcg aacggaacgg cgcatccgcc tgcgccacca cacggtaagt
     1381 acggccaaat cggttgaagt cgttgacata gagagacccc aggttgactt gcagcgtctg
     1441 gaatacgtcg ttgaggttca cgccctggcg cttgaccttg aggcgatcca catcgacgtc
     1501 gagctgcgga acgttgatct gatagctgga gaacacaccg gccagcgaag gatccgccca
     1561 ggccttttgc aacgcggtct gcacggcgcc gtacaacgct tcggcgccac ggttctgccg
     1621 gtcctccacg ttgagcttga atccgcccaa tgtgccaagc ccgatgacgg gcggtggcgg
     1681 gaataccgca atgtatgcgt cctggatgga cgcgaacttc tggttgagcg cgccggcaat
     1741 ggcgccggcg gtgagaccct tgcgctcatc gaagtccttg agcgggaaga acaccagcgc
     1801 tgcactggac tgcgcggcga aaccgttcgc cgatacgccg gcaaactgaa cggagtcgag
     1861 cactccgggc tcctgcttcg cgatctcgcc catgcggcgc accacttcgt tggtgcgatc
     1921 gatggacgcg gccggtggca actgcacgac gccaaccagg tacagcttgt cctgctgcgg
     1981 aatgaacccc gccggcgtct ggtagaagcc gagcaccgtg agcgcaacca ggccggcata
     2041 gacggcgatg gcgatcggcg cacgtttgac tgtcttcgtg gcgccgcggg aatacgcgtc
     2101 actcgcccgg gcgaacacct tgttgaagcg agcgaagaac cggccgaata ccttgtcgat
     2161 gccgcgcgtc agcgcgtccg gcttggcgcc gtgtggcttg agcagcagcg cggccagtgc
     2221 cggcgacagc gtcagcgaat tgaacgccga gatcacggtg gagatggcga tggtcagcgc
     2281 gaactggcga tagaactcgc ccgtgaggcc ttcgacgaac gccaccggaa tgaatacggc
     2341 gcacaacacc agcgtgatgg cgatgatcgg gcgactcact tcgctcatgg ccttcttggt
     2401 cgcctcgagc ggcttgagcc cgttcgctat gtggcgctcg acgttttcca ccacgacgat
     2461 ggagtcatcc accacgatgc cgatggccag caccagtccg aacagtgaca gcgtgttgat
     2521 cgagaagccg agtgcgtaga gcaccgcgaa tgtgccgatc accgacacgg gaaccgcgac
     2581 cagcggaatg atcgacgccc tccaggtctg caggaacagg atgaccacga gcacgacgag
     2641 cagcacggcc tccagcaaag tctcgaccac ggcatcgatg gactgacgta cgaactgcgt
     2701 cgggtcgtag ataatcttgt agtccatgcc ttcggggaaa ctcttcttca gggtctccat
     2761 gtcggcgcgg actttgttcg agagctcgag tgcgttcgag ccgggcgcct ggtagacagg
     2821 aatcgcgact gcgttttcgt tgccgagcag cgactgagtg gcgtaggtag ccgcgccgag
     2881 ttcgaggcgc gagatatcgc gcaaccgcac gacttcgccg ttctggccgg tcttgacaac
     2941 gacgtcgccg aactcttctt cgttttcgag tcggccttgc gtattgagcg ccagctggaa
     3001 ttcggagcct tcggcgggcg gcgcgccgat ctggccggcg gccacctgca cgttctgttc
     3061 gcggatggcc gcgaccacat caccggtggt caggtcgcgc gcggacattt tttgcggatc
     3121 gagccagata cgcatggcgt aatcgccggc gccgaacaca cgcacgtcgc ccacaccatt
     3181 caggcgggcc agcgtgtcac gcacgttcag cgtggcgtag ttgcgcaggt acagcgagtc
     3241 gtagcgtcca tcgggcgagt tcaggtgcac caccatcagg aacgcgggcg aactcttcag
     3301 cgtggaaacg ccgatggctc gcacctcttc cggcaggcgg ggcaaagcct gcgccacgcg
     3361 gttctggacc tgcacctggg cgcgatcgat atccgtgccg atgcggaacg tgacggtcag
     3421 cgccagcgac ccgtcgatgg tcgcctggga tgacatgtag agcatgccct cgacgccgac
     3481 gatttcctgt tcgagcggtg cggccaccgt ctcggaaatc accttgggat tggcgccggg
     3541 gtaggcggct ctcaccacca ccgaaggcgg cactacttcc gggtactcac tgattggaag
     3601 tttgaaaagc gagatggcgc ccgcgatgag gataaaagcc gacaggaccg atgcgaacac
     3661 cggcctgtcg atgaagtagc tcgaaaattt cattggcgtc tcgctaatta ttcggggtca
     3721 ggcaccgttt cctgggaaac ggtgcctggc accatttcag ttctgggcga ccatggtctg
     3781 gccaccgcgg cgcggctcac ccatggccac gagctgcggt gtcacgggca tgccggggcg
     3841 cacctgctgc agtcccttga ccacgatgac gtcgttcgcc ttcaggcctt cacgcaccac
     3901 gcgcaggccg tcgacgagcg ggccgagctt caccggggtg tattcgatct tgttctccgc
     3961 gccgacgcgc agcacgaact tgacgctctg gtcggtgccg atggcgctgt cgttgatcag
     4021 cagcgcgtcg tactggccgc tgccgatgag tttcacgcgc gcgaacatgc cgggggtgaa
     4081 acgccggtcg gcgttcttga acagtccgcg cgcgtgaatg gtgcccgtgt tcgggtcgag
     4141 ctcgttgtcg aggaagacca tgacgccttc gtgcggatgg ccctgttcat tggcgcttcc
     4201 gac
//
LOCUS       DASQJS010000109         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3827999, whole genome shotgun sequence.
ACCESSION   DASQJS010000109 DASQJS010000000
VERSION     DASQJS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3827999"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2229
                     /gene="leuS"
                     /locus_tag="VEW08_04425"
     CDS             <1..2229
                     /gene="leuS"
                     /locus_tag="VEW08_04425"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HYJ40012.1"
                     /translation="WNQWLFLRMLEKGIAYRKTGVVNWDPVDQTVLANEQVIDGRGWR
                     TGALVEKREIPMYYLGITRYADELLGDLDKLPEWPERVKTMQANWIGKSEGCGISFPF
                     APDTRSLMNADGALKVFTTRADTLFGASFMAIAAEHPIALAAGARNPALQTFIDECRR
                     GSTMEADIATQEKKGMRTGLHVLHPFTGQMVEVWVANYVLMAYGEGAVMGVPAHDARD
                     FEFAIKNKLTVMTVINSKTGAYDKVVEPWIDAYTEYGITVNSGVFDGLEFQAAVDAIA
                     AALEKKGLGRKRTQWRLRDWGISRQRYWGCPVPLIHCAKCGVVPVPDDQLPVVLPEGL
                     VPDGSGNPLNKTPSFYECKCPRCGADARRETDTMDTFVDSSWYFLRFACPDANTMVDE
                     RANYWLPVDQYIGGIEHAILHLLYSRFWTKVMRDMKLVEIAEPFKNLLTQGMVLAHGF
                     YRIEPTGSRTWHNPADVEVILGENNQPKEYILLTDNKPVQSDGLGTMSKNKNNGVDPN
                     GIIEQFGADTARMFMMFTAPPEQTLEWSEEGVQGSFRFIRRLWKAVFDQVNAPPPADS
                     ITTSEFDKTKLDAAQKEMRRIAHSTLAKVTDDIGRRRSFNTAVAAVMELLNAIGKFDS
                     TTLAGRVVAHEALEIAVMCLSPMIPHVTHQLWRELGHEQALATERWWAPDPDALVQDQ
                     IEISVQVNGKMRARISIPAEAAQDQAVAVALADANVQKFVDGKPIRKAIYVKGKLVNI
                     VV"
     gene            2226..2726
                     /gene="lptE"
                     /locus_tag="VEW08_04430"
     CDS             2226..2726
                     /gene="lptE"
                     /locus_tag="VEW08_04430"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HYJ40013.1"
                     /translation="MKGCQAPFPGKWCLAPFFLLIAGCGFHLQGRQPLPAQFSHTYVD
                     TRDEQTDFVQDLRKALIASKVDVIPTKNSSTAVIQVHEDELTERILSVSAQNIPTEYE
                     LTYKVTFSVLSEGKTLIEKEEISATRDISFDEAQLLAKEREQEILRAALARDLVALVM
                     RRLAAL"
BASE COUNT          557 a          857 c          898 g          489 t
ORIGIN      
        1 tggaaccagt ggctgttcct gcgcatgctc gagaagggca tcgcttatcg caagactggc
       61 gtcgtcaact gggatcccgt ggaccagacc gtgctcgcca atgagcaggt gatcgacggc
      121 cgcggctggc gcaccggcgc cctggtcgag aagcgcgaaa tcccgatgta ctacctcggc
      181 atcaccaggt atgccgacga attgctgggc gatctcgaca agcttccgga gtggcccgag
      241 cgcgtgaaga cgatgcaggc caactggatc ggcaagtccg aaggctgcgg gatctcattc
      301 ccgttcgcac cggacacccg gtcgctcatg aatgccgatg gcgcgctcaa ggtgttcacc
      361 acgcgcgcgg acaccttgtt cggcgcgtcg ttcatggcga tcgccgccga gcatcccatc
      421 gcgttggcgg ccggcgcccg gaatccggcg ctgcagacgt tcatcgatga atgccgccgc
      481 ggcagcacga tggaagccga catcgccacg caggaaaaga agggcatgcg caccgggctc
      541 cacgtgctgc atccattcac cggccagatg gtcgaggtct gggtggcaaa ctacgtgctc
      601 atggcctacg gcgaaggcgc ggtcatgggt gtgccggctc atgacgcacg cgacttcgag
      661 ttcgcaatca agaacaagtt gaccgtgatg acggtcatca attcgaagac gggcgcatac
      721 gacaaagtgg tcgagccctg gatcgacgcg tacaccgagt acggcatcac ggtgaattcc
      781 ggcgtgttcg atgggctcga gttccaggcg gcggtcgatg ccattgccgc ggccctggaa
      841 aagaaaggtc tcgggcgcaa gcgtacgcag tggcgcctgc gggactgggg catctcgcgc
      901 cagcgttact ggggttgtcc ggtgccgctc atccattgtg ccaagtgcgg cgtcgtgccc
      961 gtgcccgacg accagcttcc cgtggtgctg ccggagggcc tggtgcccga tggctcgggc
     1021 aatccgctca acaagacgcc gagcttctac gaatgcaagt gtcccaggtg cggcgcggat
     1081 gcgcggcgcg aaacggacac catggacacc ttcgtggact cgtcgtggta tttcctgcgc
     1141 ttcgcgtgcc ccgacgcgaa cacgatggtg gacgagcgcg ctaactactg gctcccggtg
     1201 gatcagtaca tcggcggcat cgagcatgcc atcctgcacc tgttgtactc gcgcttctgg
     1261 accaaggtca tgcgcgacat gaagctggtc gagatcgcgg agccgttcaa gaatctgctg
     1321 acgcagggaa tggtgcttgc gcatgggttc tatcgaatcg agcccaccgg ttcgcgcacc
     1381 tggcacaacc ccgccgacgt cgaggtgatt cttggcgaaa acaatcagcc caaggagtac
     1441 atcctgctca cggacaacaa acccgtgcag tcggatggcc tgggcacgat gtcgaagaac
     1501 aagaacaacg gggtagatcc caacggcatc atcgagcaat tcggcgccga cacggcgcgc
     1561 atgttcatga tgttcaccgc gcctcccgaa cagaccctgg agtggtcgga agagggcgtg
     1621 cagggttcgt ttcgtttcat ccgccggctg tggaaggcgg tgttcgacca ggtgaatgcg
     1681 ccaccgcccg cggattcgat caccacgagt gaattcgaca agacgaagct cgatgccgcg
     1741 caaaaggaga tgcgccgcat cgcgcattcc acgctggcca aagtcaccga cgacatcggg
     1801 cgccgccgca gtttcaacac cgcggtcgcg gcggtcatgg agctgctgaa cgcgatcgga
     1861 aagttcgaca gcacgacact ggccggacgc gtagtcgcgc acgaggcgct cgagatcgcc
     1921 gtgatgtgcc tctcgccgat gattccgcat gtcacgcacc agctctggcg cgagctcggg
     1981 catgagcagg cattggcgac cgagcgctgg tgggcgcccg accccgacgc gctggtgcag
     2041 gaccagatcg aaatctcggt gcaggtgaat ggcaagatgc gcgctcgcat ctcgatcccg
     2101 gcggaggccg cgcaggacca ggccgtcgct gtcgcgctcg cggatgcgaa cgttcagaaa
     2161 ttcgtcgatg gcaagccgat ccggaaggcc atctacgtga agggcaaact cgtgaacatc
     2221 gtcgtatgaa agggtgtcag gcaccgtttc ctggcaaatg gtgcctggca ccgtttttct
     2281 tgctgatcgc gggttgcgga tttcacctgc agggccgcca gccgttgccg gcgcagttca
     2341 gccacaccta cgtggacacc cgggacgagc aaaccgactt cgtgcaggac ctgcgcaagg
     2401 cgctgatcgc ctcgaaagtg gatgtcattc ctacgaagaa ctcttcgacc gctgtcatcc
     2461 aggtgcacga ggacgagctc acggagcgca tcctgtcggt gtccgcgcaa aacattccga
     2521 ccgaatacga actcacctac aaagtgacgt tttcggtgct ctcggaaggc aagacgctca
     2581 tcgagaagga agaaatctcc gccacgcgcg acatcagctt cgatgaagcg caattgctcg
     2641 ccaaggaacg cgaacaggaa atcctgcgcg cggcactggc gcgtgacctg gtggcgctgg
     2701 tgatgcgccg cctggcagcg ctctagcggt ttttcgtggg cccgcgatcc agtcccgccg
     2761 acgttctgcg cgtgggcaag gaggcgcacg catccgtggt c
//
LOCUS       DASQJS010000110         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1001713, whole genome shotgun sequence.
ACCESSION   DASQJS010000110 DASQJS010000000
VERSION     DASQJS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001713"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VEW08_04435"
     CDS             <1..885
                     /locus_tag="VEW08_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ40014.1"
                     /translation="AEWQREEGTSDGSLDFGGGFILPTSFELTRTSWAPFAEVRLDSS
                     FGLSTQAGVRVDNPDGYSSVTSPRVRVAYEFADSGFTVAGAWGKAFKLPSLYALGHPL
                     VGNPDLVPERGESYELELSQAVEGTAARWSATYFDGEFRNAIDFDSGPPPMLVNRNRV
                     DTSGFELAGQLGVGEQWKLDASVTNAKSRIASTGGELRNRPEWRAGLGAHWAPSAALK
                     FSAAATYVGESFDSSIATGDMDLPAYTRVDVSAVWQISPLFQAYLAIDNLTDQQYEQF
                     VGFEVRGIAPRLGVKLSF"
     gene            complement(947..1312)
                     /locus_tag="VEW08_04440"
     CDS             complement(947..1312)
                     /locus_tag="VEW08_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ40015.1"
                     /translation="MNEPKFQVIAPIFQVANLQRSIDFYTRVMGFELGWTAGEPLDRA
                     SLCRDSVEITIETDPAPARAKAYIYVSGVDEIYSRAAAAGAQVTVPLADRFYGMRDGR
                     IVDPDGNELHIGESIDIEK"
     gene            complement(1385..>2709)
                     /locus_tag="VEW08_04445"
     CDS             complement(1385..>2709)
                     /locus_tag="VEW08_04445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ40016.1"
                     /translation="VLLSLIIDRIGFKMTLWFAAVCHLLGLVLILMADGYWSLYWGTF
                     VLSLGNGAVEAGINPLIAREFRHDKTTWLNRLHAGWPAGFVLGGILAMMFPAGMGWRY
                     QMALILIPVITYIVMLLPRQFPPSERVAAGVSYREMLAEAGFVSAFIVAGLMVFELAR
                     VFGWSDAIKWGLIAALTIGYGLWSRSPGKPIFIVMALVMIPLAVTELGTDSWITSMLT
                     PAMTDLGVNAGWVLVYAMAIVLVLRLFAGPLVHRFSPLGLLAISALIAVAGLLLLARS
                     EGAVAIIVAATVYSFGKAFFWPTSLGVVAEQSPRGGAITLNVVAGIGMLGAGVIGGPL
                     IGKVLDGQMVHGVAAYDAKNNTQLVETLKIERTGLFGDYTAIDPAKRATLAGDEKAAV
                     DAADREAKHDALRTIAWLPGLMFLVYLGLILYFRSRGGYKPVVLDLKK"
BASE COUNT          523 a          852 c          885 g          449 t
ORIGIN      
        1 gcggagtggc agcgcgaaga aggtacaagc gacggttcgc tggatttcgg cggcggcttc
       61 atcctgccca cctcgttcga gctcacgcgc acgtcgtggg cgccgtttgc cgaggtgcgc
      121 ctggattcga gtttcggtct gtcgacgcag gctggagtgc gcgtggacaa ccccgatggg
      181 tattcctccg tgacgagccc gcgcgtgcgc gtggcctacg aattcgcaga cagcgggttc
      241 acggtggccg gcgcctgggg gaaggcgttc aaactaccca gcctctacgc gctgggccat
      301 ccgctggtgg gcaatccgga cctggtgccg gagcgcggcg aatcttatga gctcgagttg
      361 tcgcaggccg tggaaggcac cgcggcgcgc tggagcgcca cctatttcga cggtgagttc
      421 cgcaatgcca tcgacttcga ttccggcccg ccgcccatgc tggtgaaccg caatcgcgtg
      481 gatacgagcg gattcgagct cgcggggcag ttgggcgttg gcgaacagtg gaagctcgat
      541 gcgagcgtca cgaatgcgaa gagtcgcatc gcttccaccg gcggcgaact gcgcaatcgt
      601 cccgagtggc gcgcaggatt gggcgcgcat tgggcgccga gcgccgcgct caagttttcg
      661 gcggcggcca cgtacgtggg cgagtcgttc gactcgtcga tcgcgacggg ggatatggat
      721 ttgccggcgt acacgcgcgt ggatgtgagc gcggtgtggc agatctcgcc gctattccag
      781 gcgtacctcg ccatcgacaa cctgacagat cagcaatacg agcaattcgt cgggttcgaa
      841 gtgcgtggca tcgcgccgcg gctgggagtg aagctcagct tctgaggcgg cgttcgcgct
      901 ggactttgag ctcgatgggc aacagccgtc ccgacggtca ggcgcactat ttctcgatgt
      961 cgatcgactc gccgatgtgc agctcgttgc catcgggatc gacgatgcgg ccgtcgcgca
     1021 tgccgtagaa ccggtcggcg agcggtaccg tcacctgcgc accggcagcg gccgcgcgtg
     1081 aataaatctc atcgacgccg ctcacgtaga tgtacgcctt tgcgcgcgcc ggcgcggggt
     1141 cggtttcgat cgtgatctcg acgctgtcgc gacacaggct cgcacggtcg agcggctcgc
     1201 ccgccgtcca gcccaactcg aaacccatga cgcgcgtgta gaagtcgatg gagcgctgga
     1261 ggttggccac ctggaagatg ggcgcaatga cctgaaactt cggctcattc atgcgacctc
     1321 cggaaataaa aaaaggggac ggcggtgagg cgcgtcccct tttgctggca aatcaggaat
     1381 gtccctattt cttgaggtcg agcaccacgg gtttgtagcc gccgcggctg cggaaataca
     1441 ggatcagacc gaggtacacg aggaacatga gaccgggcag ccacgcgatg gtgcgcagcg
     1501 catcgtgctt cgcctcgcga tcggccgcat cgacagcagc cttttcatcg cctgccagcg
     1561 tcgcacgctt cgcgggatcg atggccgtgt agtcgccgaa gaggccggta cgctcgatct
     1621 tcaacgtctc caccagctgc gtgttgttct tcgcgtcgta cgccgcaacg ccatgcacca
     1681 tctggccgtc cagcaccttg ccgatcagcg gaccaccgat gactcccgcg cccagcatgc
     1741 cgatgccggc caccacgttg agagtgatgg cgccgccgcg tggcgattgc tcggcgacga
     1801 cccccagcga cgtcggccag aagaacgctt tgccgaagct gtacacggtg gccgccacga
     1861 tgatcgccac tgcgccttcg gatctggcga gcagcagcaa acccgccact gcgatcagtg
     1921 cggagatagc gagcaggccg agcggcgaga agcgatgcac cagcgggccc gcgaacaacc
     1981 gcagaaccag cacgatcgcc atggcgtaga ccaggaccca tcccgcattg acaccaaggt
     2041 cggtcatcgc aggcgtgagc attgaagtga tccagctgtc cgtgccgagc tcggtgactg
     2101 cgagcggaat catcaccagc gccatgacga tgaatatcgg cttacccggc gagcgagacc
     2161 acagaccgta gccgatggtc aacgcggcga tgaggcccca cttgatcgca tccgaccagc
     2221 cgaacacgcg cgccaattcg aacaccatga gcccggcgac gatgaacgcg ctgacgaagc
     2281 cggcttccgc cagcatctcg cgatacgaca cgccggccgc cacgcgttcg ctgggcggga
     2341 actgacgcgg cagcagcatg acgatgtacg tgatgactgg aatcaggatg agcgccatct
     2401 ggtagcgcca acccatgccg gcgggaaaca tcatggcgag aatgccgccg agcacgaaac
     2461 ccgcgggcca gccggcatgc aggcggttga gccacgtggt cttgtcgtgg cggaattcgc
     2521 gcgcgatcag tggattgatg cctgcctcga ccgcgccatt gcccagcgac aacacgaagg
     2581 tgccccagta cagcgaccaa tagccgtcgg ccatgaggat cagaaccagg ccgagcagat
     2641 gacagaccgc ggcgaaccac agcgtcatct tgaatccgat gcgatcgatg atcagcgaca
     2701 gcagcacga
//
LOCUS       DASQJS010000111        14838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_60645, whole genome shotgun sequence.
ACCESSION   DASQJS010000111 DASQJS010000000
VERSION     DASQJS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14838
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60645"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VEW08_04450"
     CDS             complement(<1..475)
                     /locus_tag="VEW08_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYJ40017.1"
                     /translation="MMYRLPAVSGEWIDRTRTLAFEFEGRAVQGFAGDTISTALAAAG
                     VMTLGRSFKYHRPRGIFSLANHDANNLFQVGGIPNVRGDVTELAAGMQVTAVNTIGGL
                     EHDRARFIEWLAPFLPVGFYYKAFHGKHFPRWEQRIRAISGLGRIDADAARERTQK"
     gene            complement(472..744)
                     /locus_tag="VEW08_04455"
     CDS             complement(472..744)
                     /locus_tag="VEW08_04455"
                     /inference="COORDINATES: protein motif:HMM:NF016179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HYJ40018.1"
                     /translation="MKVIHCPLNGPRPALEFVSAGEVRTTPDPDACSDAEWTAYLFNR
                     ASVPGVKREWWCHTPSGYWFVVERDTARDEILATYDAAELAARAGA"
     gene            complement(755..1942)
                     /locus_tag="VEW08_04460"
     CDS             complement(755..1942)
                     /locus_tag="VEW08_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ40019.1"
                     /translation="MFRQPERLKDGYDVVIIGGGGHGLAAAYYLARDHGIRDIAVLEK
                     GYLGGGNTARNTTIIRSNYLTPEGVKFYDASLALWAGLSEEFDLNLFYSQRGHFTLAH
                     SDADLRTARWRAEVNKHFGVRSEVVDAAFVKKLVPQIDLECGGHSPVLGALYHEPGAI
                     ARHDAVAWGYARGADRQGVEIHQNTEVLGIDVRDGAVAGVRTQRGTIATRRVLCAVAG
                     STPRVLKLIGLDSPLAIHPLQAMVSEPLKPWLDPIIVSSSLHAYVSQSARGELVMGAS
                     LDPQELHSTRSTLDFAEGLAVNMLDLFPFLSEVKVNRQWAGMADMTPDFSPIMGLTPC
                     EGFFLDAGWGTWGFKATPISGKTMAQTVATGTPHPLIEGFSLERFARLELTGEKGAAS
                     VGH"
     gene            2146..2730
                     /locus_tag="VEW08_04465"
     CDS             2146..2730
                     /locus_tag="VEW08_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HYJ40020.1"
                     /translation="MTRQIGATVRRLRSSMGLTLGEVAKRSNVSAGMLSRLENGDVSP
                     SLETLAALTAALGVPLGTLFGDIGKPRGGAQHVPKGQGLEVVRRGTKRGHTYHLLAAD
                     RGPRRAFEPFLVTLNDKSEVFPGFEHPGTEFIYLLEGSLTYRHGDETHLLKVGDALTF
                     IGSVPHGPEKLLKTPIRMLSIIIYADGPDHVLTA"
     gene            2826..4217
                     /locus_tag="VEW08_04470"
     CDS             2826..4217
                     /locus_tag="VEW08_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HYJ40021.1"
                     /translation="MSSVAAAAGGPATGIKHPPGPRGLPVIGCLLPFLKNPMEALTHI
                     ARTYGGLARVPIRGKYLYVLSDPEMLKEMLITHRTKYMKNIRYRHIQSLLGQGLLLSE
                     AADWRRQRVITQPAFKPEAIDNSVAWMAEHVDHFLNRWDGYADRGEIFDVEPEFNRLA
                     QLLSGRYLLGEPFADIADEFCEVAAAVKRHWPQAPRNIVRAFIPHSKELIARFDAAIA
                     ALDSCILGFIKRHRATDFEGCGVLKRIVATSRAEGNEYDDKSLRDQISTLFFAGHETS
                     ATGLCWIHWALSRHPAIRAQMQAEITQVLGDRLATAEDVHNLQYTGQVIDESLRLHSP
                     IHSISRVAMVDNTVGGYHVPAGATVAVSMYAIHRLAHLYPNPEVFDPSRFTPEQVAAR
                     HRFAYLPFAAGHRNCIGAGQALVELKLIVARIAQRFELDLAPGHKVEPAPGTTMFPRY
                     GMKMRIRHVKAAP"
     gene            4214..4969
                     /locus_tag="VEW08_04475"
     CDS             4214..4969
                     /locus_tag="VEW08_04475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ40022.1"
                     /translation="MSVPMDSRSEQARQFYNAAAATSTDFAFMNYGYAPLSSDLEGTA
                     EPEKYCLQLYRRLLGNADLRGKKVVEVSCGRGGGAAHVAATYQPASYLGIDISERNLN
                     LATQRFSGVTALSFQAGNAEALPLPDASCDALLNVEASHLYDNPARFFAEAFRVVKPG
                     GTFFHVDLAWKDKDPPAMIAAAGFQIASVEDITKNVVMALDLDSVRREAIVMSFPENM
                     RKDFRDWSGVKDFRAYNRLANGEWIYRITRGTR"
     gene            5070..7268
                     /locus_tag="VEW08_04480"
     CDS             5070..7268
                     /locus_tag="VEW08_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HYJ40023.1"
                     /translation="MNLSRSLLVLSALVGLVSPVFAQAPQPDFARDVQPIFKKHCYEC
                     HGPDKHKNGYRLDRRSAAFGGLVRHNIIAGNSDSSRLYRRVLNSRLGPQMPLEDTLSE
                     EQIDTLRRWIDAGAHWPDELANESDLPPPDAAAVQLAALVRTSTFDKSSRRKALAMVR
                     AQPSIVNARGVGGTPPLMEAALYGDVKLLAAMLDAGGDPNLRNYRATSALMWAVDDIA
                     KVRLLLDRGADPNASSDFGATPLTLAAASKDSAPLVQLLLDRGAEATLPALVAAARAN
                     PDAVRILLARVPDKGGAAATMAVRARCDECLAILQPDPKSPMPRVLAILLPPGSAGDP
                     QMLRAAMLRSPDVNVIDPRDRSPLMQAAISESVPPDVMQEIIARGANVHAVSLDGLNA
                     LDYARTLGRASTIDVLTRAGAKPTRAGDEKPLEYVRGNDTHAAVARSMPLLLRSSETF
                     YEKGGCVSCHHNLLGLVTAQTLRRQGLPLDEKIAASELRVLAEDMATSRDQALQGIVV
                     PGGLFTTTGYILIGLAAGGHPADAATDALVRLLRRGQFPDGRWVSPVRPPSEASMFTA
                     TAVSLRGIQLYGNPRSAADRAAIASATAWLRKAAPENTEDATFQLLGLTWARAPASER
                     RAAIDALLAAQKPNGGWAQLDYRAPDAYATGQVLVALHEAGVSARSPAYQRGVRYLLD
                     TQLTDGSWLVPSRTLPTQIYFESGFPHGVHQFISAAGTQWATQALAWSIK"
     gene            7556..11137
                     /locus_tag="VEW08_04485"
     CDS             7556..11137
                     /locus_tag="VEW08_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HYJ40024.1"
                     /translation="MAWQFWIDRGGTFTDIVARRPDGALLTHKLLSESARYHDAAVQG
                     IRDLLELAPTAPLPTELIDVVRMGTTVATNALLERKGDRTLLVTTRGFADALRIAYQN
                     RPKIFARRIELPSMLHEEVVEIDERIGARGEIVRPLDADAAEAALLAAQARGLRACAI
                     VLMHGYRYPAHEEALASIATRVGFEQISTSHQVSPLIKLVARGDTTVADAYLSPVLRR
                     YVDRVAAALPGTRLQFMQSSGGLSDAQSFHGKDAILSGPAGGIVGAARAAQSAGFKKV
                     IAFDMGGTSTDVGHFAGEYERALDVEVAGVRLRTPILRIHTVAAGGGSIVSFDGVRLR
                     VGPDSAGANPGPASYRRGGPLTVTDCNVLLGRIQPDFFPSLFGQTGTEPLDAAVVQSQ
                     FAILAQSMPGTQNTATLAAGCIRIAVQNMANAIKKISVQRGHDITDYALTCFGGAAGQ
                     HACLVADALGMRSVLIHPLAGVLSAYGIGLADTIVMRQQTAEVPLNAEALEQLGAAYA
                     ALEHEAGAELVLQGFHASAVQLERSVLLKYSDTDSTLTLSLLPDSTPETLTVEFLAQY
                     RTRFGFHVPGRAIVVDTIGVTATGRSPDIPTASRNFAPRAGALAPIVSRPVFFDGAWL
                     DAPFFERESLRPGDAIAGPAVICERNTTVVVEHGWRATLTEADNLVLAKAATSRQETV
                     STRADPVLLEIFNNLFMAIAEQMGVTLANTASSVNIKERLDFSCALFDAQGQLIANAP
                     HMPVHLGSMGESVQEILRRRGRDIQPGDAFALNAPYAGGTHLPDVTVVMPVFLGVGGP
                     RFFVAARGHHADIGGITPGSMPSDSKTIDEEGVLIDSERIVDRGVFLEEKLRALLTTG
                     RWPVRNVTQNLADLRAQIAACQKGIAELSAMTGHYGLNVVQAYMQHVQDNAEAAVRKV
                     IDVLKDGAFTCEMDNGAVVRVAIRIDHARREATIDFTGTSAQQPNNFNAPLPVTRAAV
                     LYVFRTLVDDEIPMNAGCMKPLHLIVPEGSMLAPRYPAAVVAGNVETSQVVTDCLYGA
                     LGVLAASQGTMNNFTFGDDEHQYYETISGGSGAGPDFDGTDAVQTHMTNSRLTDPEVL
                     EWRFPVRLESFRIRRGSGGAGHHRGGDGVERRLRFLKPMTAVMLANHRRVAPFGVAGG
                     EPAAVGHNWIERATGGTEPFGAAFSTAVQRDDVIVIQTPGGGGFGKK"
     gene            11134..12408
                     /locus_tag="VEW08_04490"
     CDS             11134..12408
                     /locus_tag="VEW08_04490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ40025.1"
                     /translation="MNNAPNMKRLAFASMAGTALEYYDFAVYNTLAALVFNKLFFPAV
                     DPLTGTLLAFATYWVGYLSRPFGGILFGNLGDRRGRRHVLVITLLIMGASTTLIGLLP
                     TYEQVGVFAPILLVALRFVQGMALGGEWAGAVLLTVEHGTDRQRGRNASFAQMGPSTG
                     VLIATGAMALITWLMPDEQLFDWGWRIPLLASVLLVAFGLWLRLGVPETPEFIALERA
                     AQQSRAPIGEVVTGHWRRLLIAGGSRFGTDVMYSLISAFCVFYVTTILGMSRTLATVA
                     LAIGAFLNVVFIFAAGTLSDRFGRRLVYGLGAAAAAVWLCLLFPLLDSKSELAVIAAF
                     ASGLAIHAFMYGPQGAFIAEQFPVRVRYVGSSVAYTFAGVFAGGLAPLAFTKIYQASK
                     ETGGIVVYAGLALIITVVALLSSRQSDGKRLG"
     gene            12412..13377
                     /locus_tag="VEW08_04495"
     CDS             12412..13377
                     /locus_tag="VEW08_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40026.1"
                     /translation="MTSKIVLALVLAVAVAVGAVYFFRDPPVTAPAVTAETMQALPPA
                     RAIGEPPQVNDKPAIEAGTTVASSDPFEFIRALAKTAFDGDGRAQYLVGREYDRCEMT
                     LSLVRKMDGDPEAVIWGFPDSWPQTLKERSIAEYRRCIRLMKEDPFAALPPRKGGYSP
                     KYWMQRSAEAGYPLAVAERSLAGAVLTSTEDAAAVGKLSVESVGELTKATMSGDPDVA
                     LTIGFRQSWFDDPARTTAASAWMLAACRMGADCGADSKVYPFFMCYDPNHPNCDKDGN
                     VELMVSASLSPEKYADAYAQSQVIEEALRNRDSAAIEALLQKFAR"
     gene            complement(13374..13820)
                     /locus_tag="VEW08_04500"
     CDS             complement(13374..13820)
                     /locus_tag="VEW08_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HYJ40027.1"
                     /translation="MFTKTLYAGWSDMDFNAHMKNTAYLDKAADVRQMFLMEHGFPVE
                     EFLRLRIGPVVMKDEVEYFKEVGLQQKIDVNYALAGHSPDGSRFLLRHEIFRADGKLA
                     ARVTSAGGWLDLAARKLIAPPPALLAAMNLLDRTGDFVELPSSIRR"
     gene            13977..>14838
                     /locus_tag="VEW08_04505"
     CDS             13977..>14838
                     /locus_tag="VEW08_04505"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HYJ40028.1"
                     /translation="MRAFNTWFAIAFAALASSLFTGCDDDKKAVAPSITAPASPLGNG
                     VVGSAISPVDFVTSGTVPITYSVSAGILPPGLALDPTTGRLSGSPTLVGIFEFTITAT
                     NRAGTDDAQYMQTILRLPTITTPTSPLAAALGGAPFTSLTFEASGSMPITWSVTAGTL
                     PPGMSLNAATGAYSGTPTAAGDYSFTVTATNAAGSDSDAFTQKVTAPAANAHVLINGN
                     SIAAVATTFPSGLDTPLSLTGLNAGDALVSIDRRPQNGFLYGLGYNAAAGSVQLYSIS
                     STTGVAAPLGA"
BASE COUNT         2646 a         4921 c         4690 g         2581 t
ORIGIN      
        1 gcttctgcgt gcgttcgcgc gcggcgtccg cgtcgatgcg gcccaggccg gagatcgcgc
       61 gaatgcgctg ctcccaacgc ggaaaatgct tgccgtgaaa agccttgtag tagaacccca
      121 ccggcagaaa cggcgccagc cattcgatga aacgcgcgcg atcgtgctcg aggccaccga
      181 tggtgttcac ggcggtcacc tgcatgccgg cggcaagttc ggttacatcc ccgcgcacat
      241 tggggatacc cccaacctgg aacaggttgt tggcgtcatg attcgcgagc gaaaagatgc
      301 cgcggggacg gtgatacttg aaactgcggc cgagcgtcat cactcccgcc gcggcgagtg
      361 cggtggaaat ggtgtcgccc gcgaaaccct ggaccgcgcg gccctcgaac tcgaatgcga
      421 gcgtccgcgt gcggtctatc cactcgccgg aaacggctgg caggcgatac atcatgcgcc
      481 ggccctcgcc gccagttccg ccgcatcgta ggtcgcgaga atctcgtcgc gcgccgtgtc
      541 acgttcgacg acaaaccagt aacccgaggg tgtatggcac caccattcgc gtttcacgcc
      601 gggcacgctg gcgcggttga acagatacgc ggtccattcg gcatcactgc aggcgtcggg
      661 atcaggcgtc gtgcgcacct cgccggcact cacgaattcg agcgcggggc gcggaccatt
      721 gagcgggcaa tgaatgactt tcacgtgttc atgctcagtg gcccaccgag gccgcgcctt
      781 tctcgcccgt gagttcgagc cgagcgaagc gttcgagcga gaaaccctcg atcagcggat
      841 ggggcgtgcc agtagccacg gtctgcgcca tcgtcttgcc cgagatgggc gtggccttga
      901 aaccccaggt gccccagccc gcatcgagaa aaaatccctc gcagggcgtg aggcccatga
      961 tcggcgagaa atccggcgtc atgtcggcca tgccggccca ttgccggttc actttcactt
     1021 ccgagaggaa cgggaacagg tcgagcatgt tcacggccag gccttcggcg aaatccagcg
     1081 tcgaacgcgt ggagtgcagc tcctggggat cgagcgatgc gcccatgacc aactcgccgc
     1141 gcgcgctctg cgacacgtaa gcgtgcagcg aactcgatac gatgatggga tcgagccagg
     1201 gcttgagcgg ctcggacacc atggcctgca gcgggtggat ggccagcggc gaatcgagcc
     1261 cgatgagttt caacacgcgc ggcgtgctgc cggccaccgc gcacagcacg cgcctggtgg
     1321 cgatggtccc gcgctgggtc cgcacgcccg cgaccgcgcc atcgcgcacg tcaatcccca
     1381 gaacctcggt gttctggtga atttcgacgc cttgacgatc cgcaccccgc gcataccccc
     1441 aggcgaccgc gtcgtggcgc gcgatcgcgc ccggctcgtg atacagcgcg cccaggaccg
     1501 gcgagtggcc accgcattcg agatcgattt gcggtacgag cttcttcacg aacgcggcgt
     1561 ccaccacctc ggagcgcacg ccaaagtgtt tgttgacctc ggcgcgccag cgcgcggtac
     1621 gcaggtcggc gtccgaatgc gccagcgtga aatgcccgcg ctgcgaatag aacaggttca
     1681 ggtcgaactc ttcggagaga cccgcccaca gcgcgaggct ggcgtcgtag aacttcacgc
     1741 cctcgggcgt gaggtagttc gagcggatga tcgtggtgtt gcgagcggta ttgccgccgc
     1801 cgagatagcc cttttcgagc accgcgatgt cgcggatgcc atgatcgcgc gcgaggtagt
     1861 acgcggcggc gaggccatga cccccgccac cgatgatgac gacgtcgtag ccatccttga
     1921 gccgttccgg ctgccggaac atgcgtggtt ctttgtgacg aaaagcgaat ttcagcaggc
     1981 gatggggcat cgaatcgata gttgcatgag agtaaattgt gttgcgcacg gataacatac
     2041 cggggcgcgg cgtacaagtc cgccagcaca agagataagg agtgggttat ggcaaaagcg
     2101 agggggcctt cggcgggacc cgggggcagt ccaggcggag gcgaagtgac gcggcaaatt
     2161 ggcgcgaccg ttcgccggct gcgcagctcg atgggactca cgctgggtga ggtggccaag
     2221 cgctcgaacg tcagcgccgg catgttgagc cggctggaaa acggcgacgt gtctccgagc
     2281 ctcgaaacgc tcgccgccct caccgccgcg ctgggggttc cactaggcac gctgttcggc
     2341 gacatcggca agccgcgcgg cggcgcgcag cacgttccga aaggccaggg tctcgaagtc
     2401 gtgcgtcgcg gcacgaagcg cggtcacacc taccacttgc tggctgcgga tcgcggcccg
     2461 cgccgcgcat tcgagccgtt tctggtcacg ctcaatgaca agagcgaggt gtttcccggg
     2521 ttcgagcacc cgggaaccga gttcatttat ctattggaag gctcgctgac ctaccgtcac
     2581 ggcgatgaaa cacatctgct gaaagtcggc gacgcgctga cgttcattgg ttcggtaccg
     2641 cacggaccgg aaaaactgct caagactccg atccgaatgc tgtcaatcat catttatgcg
     2701 gatggtccgg atcacgtgtt aaccgcatga gcccccgaaa attgcgctac gctcggcagc
     2761 ttcgataatt acaaaaaact aataatctga cgctcggggt ccacaggggg gcgtaggtga
     2821 attcagtgag ttctgttgca gctgcggctg gcgggccggc cacggggatc aaacatccac
     2881 cgggcccacg tggtctgccg gtcatcggct gcctgctgcc tttcctcaag aacccgatgg
     2941 aagcgctgac tcatatcgcg cgaacgtacg gcggtctcgc gcgcgtaccc atccgcggca
     3001 agtacctcta tgtgctctcg gatcccgaga tgctcaaaga gatgctgatc acgcatcgaa
     3061 cgaagtacat gaagaacatc cggtaccggc acatacagtc gctgctgggc cagggactgc
     3121 ttctctccga ggccgccgac tggcggcgcc agcgagtcat cacgcagccg gcgttcaagc
     3181 ccgaagccat cgacaactcg gtcgcctgga tggccgaaca cgtcgatcat ttcctgaatc
     3241 gctgggatgg ctacgccgat cgcggcgaga tcttcgacgt cgaaccggag ttcaaccgcc
     3301 tggcgcagct gctgtcgggc cgatatctat tgggcgagcc gttcgccgac atcgccgacg
     3361 aattctgcga agtggccgcg gccgtcaaga gacactggcc gcaggcgccg cgcaatatcg
     3421 tgcgcgcgtt cataccgcat tcgaaagagc tgattgcgcg cttcgacgcc gccatcgccg
     3481 cgctcgactc ctgcatcctg gggttcatca agcggcaccg agccacggat ttcgagggct
     3541 gcggcgtcct caagcgcatc gtcgccacga gccgtgccga gggcaacgag tacgacgaca
     3601 agtcgctgcg cgaccagata tccacgctgt tcttcgccgg tcacgagact tcggccacgg
     3661 ggctttgctg gatccactgg gcgctgtcgc ggcacccggc cattcgcgcg cagatgcagg
     3721 ccgagatcac ccaggttctg ggcgaccggc tcgcaaccgc tgaggatgtg cacaatctgc
     3781 aatacaccgg ccaggtcatc gacgaatcgc tgcgcctgca ttcaccgatt cactccatct
     3841 cgcgcgtcgc gatggtcgac aacaccgtgg gcggttatca cgtcccggca ggcgccaccg
     3901 tcgccgtctc gatgtatgcc atccaccggc tcgcacactt gtatccgaat cccgaggtgt
     3961 tcgatccctc gcgctttacg cccgagcagg tggccgcgcg tcatcgcttt gcctacctgc
     4021 cgtttgcggc cggccatcgc aactgcatcg gcgccggcca ggcgctggtg gagctcaagc
     4081 tcatcgtcgc gcgcattgcg cagcgcttcg agctcgatct cgcgccgggt cacaaggtcg
     4141 aaccggctcc cggcaccacc atgtttccgc gctatggcat gaagatgagg atccggcacg
     4201 tcaaggcagc gccatgagcg tgccgatgga ttcacgcagc gagcaggcgc ggcagttcta
     4261 caacgcggcc gcggcgacct cgaccgattt cgccttcatg aactacggct atgcgccgtt
     4321 gtcgtccgat ctcgagggca ccgcggagcc ggaaaaatac tgcctgcagc tctaccggcg
     4381 tttgctgggt aacgcggacc tgcgcggcaa aaaggtagtg gaagtgagct gcggacgcgg
     4441 tggcggtgcg gcgcatgtcg ccgccacgta tcagcccgcg agctacctcg gcatcgacat
     4501 cagcgaacga aacctgaatc tcgccacgca gcggttctcg ggcgtgaccg ccctgagctt
     4561 ccaggcgggc aacgcggagg cactaccgct gcctgacgcg agctgcgacg cgttactcaa
     4621 cgtggaagcc tctcatctgt atgacaaccc ggcacgtttc ttcgccgaag cgtttcgcgt
     4681 ggtgaagccg ggcggcacgt tcttccacgt ggacctcgcg tggaaagaca aggatccgcc
     4741 agcgatgatc gccgccgccg ggttccagat cgcctcggtg gaagacatca cgaagaacgt
     4801 cgtgatggcg ctggatctcg acagcgtgcg gcgcgaagcc atcgtcatga gcttccccga
     4861 gaacatgcgc aaggatttcc gcgactggtc aggcgtgaag gatttccgcg cgtacaaccg
     4921 cctggcgaat ggcgagtgga tttaccggat tacccgcggc acccgctagt ggggacagtc
     4981 gatacgtcgc cttgtaatga ttcttcgtga cccggcagaa cttgagggtt accgtgcgca
     5041 aaagcacggt caccgttgct gaggtcgcct tgaatctgtc ccgctccctc ctggtcctgt
     5101 cggctctcgt cggcctcgtc tcccccgtgt tcgcgcaagc accgcagccg gatttcgcgc
     5161 gcgacgtgca gccgatattc aagaagcact gctacgagtg ccacggcccc gacaagcaca
     5221 agaacggcta ccggctcgac cggcgcagcg cggcgttcgg cggcctggta cgtcacaaca
     5281 tcatcgcggg caacagcgat tcgagccgcc tgtatcggcg ggtgctcaat tcgcgcctgg
     5341 ggccgcagat gccgctcgag gacacgctct cggaggagca aatcgacacg ctgcgccgat
     5401 ggatcgatgc tggcgcgcac tggcctgacg aactcgcgaa cgagagcgac ctgccgccgc
     5461 ctgacgcggc cgcggttcag ctcgcggcgc tggtccgcac ttccaccttc gacaagtcga
     5521 gccgccgcaa ggctttggca atggtgcggg cacaaccgtc catcgtgaac gcacgcggcg
     5581 tcggtggcac gccgcccctc atggaagccg cgttgtatgg cgacgtcaaa ctactcgctg
     5641 cgatgctcga cgcgggaggc gatcccaacc tgcgcaacta tcgagccacg agcgcgctca
     5701 tgtgggcggt ggacgacatc gccaaagtgc ggttactgct cgaccgaggc gccgatccga
     5761 atgcgtcatc ggacttcggc gcgacgccac tcacgctggc ggcagcttca aaggattccg
     5821 cgccgctcgt acaactgctg ctcgatcgcg gcgccgaagc cacgctaccg gcactcgtcg
     5881 ccgccgcgcg cgccaatcca gacgcggtgc ggatattgct ggcgcgagtc ccggacaaag
     5941 gcggcgcggc agccacgatg gcggtacgcg cgcgttgcga cgagtgcctg gcaattctgc
     6001 agcccgatcc aaaatcgccg atgcctcgcg tgctggcgat tctgctgccg cccgggtcgg
     6061 cgggcgatcc gcagatgctg cgcgccgcga tgctgcgcag tcccgacgtc aatgtcatcg
     6121 accccagaga ccgctcgccg ttgatgcagg ccgccatctc ggaatccgtg ccaccggacg
     6181 tcatgcagga aatcatcgcg cgcggtgcca acgtgcacgc agtcagcctg gatggcctga
     6241 atgcgctcga ctatgcgcga accctcggca gagcttccac gatcgatgtc ctcacccgcg
     6301 ctggcgcgaa acccacgcgc gcaggcgatg agaaaccatt ggaatacgtg cgcggaaatg
     6361 acacccacgc cgcggtcgcg cgcagcatgc cactgctcct gcgcagctcc gagacgtttt
     6421 atgaaaaggg cggctgcgtt tcctgccacc acaatctgct gggactggtg accgcgcaga
     6481 cgctgcggcg ccaggggctt ccgctcgacg agaagatcgc ggccagcgag ctgcgcgtgc
     6541 tggccgaaga catggccacc tcgcgcgatc aggcgctgca gggcatcgtc gtgccaggcg
     6601 gcctgttcac gaccacgggt tacatcctga tcggcctcgc agcgggcggg caccccgccg
     6661 acgcggccac cgacgcgctc gtcaggctcc tgcgccgcgg gcagtttccg gatggacgct
     6721 gggtcagccc ggtgcgcccg ccgagcgagg ccagcatgtt caccgcgacg gcggtttccc
     6781 tgcgcggcat ccagctctat ggcaatccgc gcagcgccgc ggaccgcgcc gcgattgcga
     6841 gcgcgactgc ctggctgcgc aaggcagcgc cagagaatac cgaggatgcg acctttcaat
     6901 tgttaggcct cacctgggcg cgcgcgccgg ccagcgagcg ccgcgcggca atcgacgccc
     6961 tgctcgccgc gcagaaaccc aacggcggtt gggcacagct cgactatcgc gccccagacg
     7021 cgtatgccac cgggcaggtg ctggtggcgc tgcatgaagc gggagtctcg gcgcgatcgc
     7081 ccgcctatca gcgcggtgtg cgctatctgc tcgacacgca gctcaccgat ggttcctggc
     7141 tcgtgccctc acgcacgctg cccacgcaga tctacttcga gagcgggttc ccacacggcg
     7201 tacaccagtt catctccgct gcaggcacgc agtgggcaac gcaagctctc gcctggagca
     7261 tcaaataaac gaggacggtg acccgtgcgc ctcttctctt ttcaccgccg ctgagagagc
     7321 gggccgggat acctggcctc gttaggcacg aagccgcctc tccgcttcat caggactctc
     7381 gctgactttc caatccaacc gcacgaaagt gtcattgcgt gtggcggcgg cccgcaatcc
     7441 agcgatgcca ccccgaacga agggcccggc gaaaagcgaa cgaactcctg tgcgcggact
     7501 gctcgctggg gcactcacac cctcaatcca cgcctccgtt tgctagcatg ccggtgtggc
     7561 atggcaattc tggatcgatc gcggcggcac attcacggac atcgtggcgc gacgccccga
     7621 cggcgccctc ctcactcaca agcttctttc tgaaagcgcg cgctaccacg acgccgcagt
     7681 ccagggcatt cgcgatctgc tcgaactcgc tccgacggcg ccactgccca ccgaactcat
     7741 cgacgtcgtg cgcatgggca ccacggtggc gaccaacgcc ctgctcgagc gcaagggaga
     7801 tcgcactctc ctcgtgacca cgcgtggctt cgccgacgcg ctgcgcatcg cctaccagaa
     7861 tcgcccgaaa attttcgcac gccgcatcga gttgccttcc atgctccatg aagaggtggt
     7921 ggagatcgat gaacgcatcg gcgcgcgcgg ggaaatcgtt cgcccgctcg acgccgacgc
     7981 cgccgaagcc gcgctgctcg ccgcgcaggc ccgaggcctc cgcgcctgcg ccatcgtgct
     8041 gatgcacggc taccgttatc ccgcacacga agaggcgctg gccagcatcg cgacgcgcgt
     8101 cggcttcgag cagatttcca cgtcccatca ggtcagcccg ctgatcaaac tggttgctcg
     8161 gggcgacacc accgtggccg acgcctatct atcacccgtg ctgcgacgct acgtggaccg
     8221 cgtcgccgcg gcactaccgg gcacgcgcct gcagttcatg cagtcctcgg gcggcctgag
     8281 cgacgcgcaa tcgttccacg gcaaggacgc gatcctgtcg gggccggccg gcggcatcgt
     8341 cggcgccgcg cgggcagccc aatcagcggg cttcaagaaa gtcatcgctt tcgacatggg
     8401 cggcacgtcc accgatgtcg ggcacttcgc cggcgaatac gagcgcgcgc tcgacgtgga
     8461 agtggccggc gtgcggctgc gcacaccgat cctgcgcatt cacaccgtgg cggcgggtgg
     8521 cggctccatc gtgagcttcg atggggtgcg cctgcgcgtg ggccccgatt ccgcgggcgc
     8581 gaatcccggc cccgcctcgt accggcgcgg cggcccgctc accgtcacgg attgcaatgt
     8641 cctgctcggc cgcattcagc ccgacttctt cccatcgttg tttggccaaa cgggcacaga
     8701 gccgctcgat gccgctgtcg tacagagcca gttcgcgatc cttgcgcaat ccatgcccgg
     8761 cacgcagaac acggcgacgc tcgccgcggg ctgcatccgc atcgcggtgc agaacatggc
     8821 caacgccatc aagaagatct ccgtgcagcg cgggcacgac atcaccgact acgcactcac
     8881 ctgcttcggc ggcgccgcgg gccagcacgc ctgcctggtc gccgacgcgc tcggcatgcg
     8941 cagcgtgttg attcatccgc tggccggcgt tctctcggcc tatggcatcg gcctcgcgga
     9001 cactatcgtg atgcgccagc agacggccga agtgccactc aacgccgagg cacttgaaca
     9061 gttgggcgcc gcatatgccg cactggagca cgaagccggt gcggagctgg tactgcaggg
     9121 gttccatgcc agcgcggtac aactcgaacg ctcagtgttg ctcaaatatt cggacaccga
     9181 ttcaacgctg acactgtcgc tgctgccgga ctcgactccc gagacactga ctgttgaatt
     9241 cctggcgcag taccgcacgc gcttcggctt ccacgtgccg gggcgcgcga tcgtcgtcga
     9301 caccatcggc gtgaccgcaa ccgggcgttc gcccgatatt ccgaccgcat cgcgcaactt
     9361 tgcgccgcgc gcaggcgcgc ttgcgccaat tgtcagccga cccgtcttct tcgatggcgc
     9421 ctggctcgac gcgccgttct tcgaacgcga gtccctgcgt ccgggcgatg cgattgcggg
     9481 ccccgccgtg atctgcgagc gcaacaccac cgtggtcgtc gagcatggat ggcgcgcaac
     9541 attgaccgaa gccgacaacc tggtgttggc gaaagcggcc acctcgcggc aggaaacggt
     9601 gtcgacgcga gcggaccccg tgttgctgga gatcttcaat aacctgttca tggccatcgc
     9661 cgagcagatg ggcgtgacgc tggccaacac cgcgagctcc gtcaacatca aggagcgcct
     9721 ggacttctcc tgcgcgctgt tcgacgcgca ggggcagctc atcgccaacg ccccgcacat
     9781 gcccgtgcac ctggggtcca tgggcgagtc cgtgcaggaa atcctgcgcc ggcgcggccg
     9841 cgacattcag ccgggcgatg catttgcatt gaacgcgccg tatgccggag gcacgcacct
     9901 gcccgatgtc acggtggtca tgcccgtatt cctcggcgtg ggcggtccca ggttcttcgt
     9961 cgcggcgcgc ggtcaccacg cagacatcgg cggcatcacg ccgggctcga tgccgtcgga
    10021 ctcgaaaacc atcgacgaag aaggcgtgct catcgacagc gagcgcatcg tcgatcgcgg
    10081 cgtgttcctc gaagagaagc tgcgggcgct gctgaccacg ggccgctggc ccgtgcgcaa
    10141 cgtcacgcag aatctcgccg acctgcgcgc gcagatcgcc gcctgccaga agggaatcgc
    10201 ggaattgagc gcgatgaccg gacactatgg tctcaacgtc gtgcaggcgt acatgcagca
    10261 cgtgcaggac aatgcagagg cggcggtccg caaagtcatc gatgtactca aggatggcgc
    10321 atttacctgc gagatggaca acggcgccgt ggttcgcgtc gcgattcgca tcgaccatgc
    10381 gcgacgcgaa gccaccatcg atttcaccgg cacgagtgcg cagcagccca acaacttcaa
    10441 cgcgccgttg ccagtgacac gcgccgcggt gttgtacgtg ttccgcacgc tcgtggacga
    10501 cgaaatcccg atgaacgccg gctgcatgaa accgctgcac ctcatcgtgc ccgaaggttc
    10561 gatgctggcg ccccgatacc cggccgcggt cgtcgccggc aatgtcgaaa cttcccaggt
    10621 cgtgacagat tgcctctatg gcgcgctcgg cgtgctcgca gccagccagg gcacgatgaa
    10681 caacttcacg ttcggggatg acgagcatca gtactacgag accatctccg gcggctcggg
    10741 cgccggcccg gacttcgacg gcaccgatgc cgtgcagacg cacatgacca actcgcggct
    10801 cacggatccg gaggtgctgg agtggcgctt tcccgtgcga ctggaatcgt ttcgcatccg
    10861 gcgtggctcg ggtggcgcgg gacaccatcg cggcggcgac ggcgtggaac ggcgcctgcg
    10921 attcctgaag cccatgaccg ccgtgatgct ggctaaccac cgccgcgtgg ctccgtttgg
    10981 agtggccggt ggtgagcccg cggcggtggg ccacaactgg atcgaacgcg cgacgggcgg
    11041 caccgaacca ttcggcgcgg cgttctcgac cgccgtgcaa cgcgacgatg tcatcgtcat
    11101 ccagacaccc ggcggcggcg gattcgggaa aaaatgaaca acgcaccgaa catgaagcgc
    11161 ctggcgttcg ccagcatggc gggcaccgcg ctcgagtatt acgacttcgc ggtgtacaac
    11221 acgctcgcgg ctctggtgtt caacaagctg ttttttccgg cagtcgatcc gctgaccggc
    11281 accctgctgg cgttcgcgac ttactgggtg ggatatctgt ccaggccctt cggcggcatc
    11341 ctgttcggaa atctcggtga tcgacgcggt cgccgtcatg tactcgtgat cactctgctc
    11401 atcatgggcg ccagcaccac gttgatcggc ctgctgccta cctacgagca ggtgggcgtg
    11461 ttcgcgccca tcctgctggt ggcgctgcgt ttcgtgcagg gcatggcgct tggtggagag
    11521 tgggctggcg cggtgttgct cacggtcgaa cacggcacgg atcggcaacg tgggcggaat
    11581 gcctccttcg cgcagatggg gccgtcaacc ggtgtgctca tcgccactgg cgcaatggcg
    11641 ctcatcacct ggctgatgcc cgacgaacag ctgttcgatt ggggatggcg aataccgctg
    11701 ctggccagcg tgctgctggt tgcattcgga ctatggctgc ggctgggcgt tccggaaacg
    11761 ccggaattca tcgcgctgga acgggcggcg cagcagtccc gcgcacccat cggcgaagtg
    11821 gtgaccggcc actggcgacg cctgctgatc gcgggtggct cgcgtttcgg aaccgacgtg
    11881 atgtattcgc tgatcagcgc gttctgcgtc ttctacgtca ccacgattct cggcatgtcg
    11941 cggacactgg caaccgtcgc gctggcgatt ggtgcgtttc tcaacgtggt gttcatcttc
    12001 gccgccggca cactttcgga tcgtttcggc cggcgcctgg tctacggcct tggcgccgcg
    12061 gctgcggcgg tctggttgtg cctgcttttc ccgctgctgg attcgaaatc ggagctcgcc
    12121 gtcatcgcgg cgtttgccag cggcctggcc atacatgcgt tcatgtacgg accgcagggc
    12181 gccttcatcg ccgagcaatt tccggtgcgg gttcgctacg tgggatcgtc ggtggcgtac
    12241 accttcgccg gcgtgtttgc cggcggactc gcgccgctgg cgttcacgaa gatttaccag
    12301 gcgagcaagg aaaccggggg catcgtcgtt tacgcggggc tggcgctgat catcacggtc
    12361 gtcgcgctgc tctcgtcgcg ccagtcggat gggaagaggc tcggataggt aatgacttct
    12421 aaaatcgtgc tcgccctggt actggcggtc gctgtggccg tcggggccgt ctatttcttt
    12481 cgtgatcctc ctgtcacggc accagcggtc acggcggaaa ccatgcaagc gttgccgcct
    12541 gcgcgcgcga tcggcgagcc accgcaggtc aacgacaaac cggctatcga agctggcact
    12601 accgtcgcca gttccgatcc gttcgagttc attcgcgcgt tggccaagac cgccttcgat
    12661 ggcgacggac gcgcgcagta cctcgtgggg cgcgagtacg accgctgcga gatgaccctt
    12721 tcgctggttc gcaagatgga cggcgatccg gaagccgtca tctggggttt tccggattcg
    12781 tggccccaga cattgaagga acgcagcatc gccgaatacc ggcgatgcat ccggctcatg
    12841 aaggaagatc catttgcggc gttgcctcca cgcaaaggcg gctactcacc caagtactgg
    12901 atgcagaggt ccgccgaagc gggctatcca ctggcagtcg ccgagcgatc gctggctgga
    12961 gcggtgctga cgtcgacaga ggacgcggcc gcagtcggca agctgagtgt cgaatcggtg
    13021 ggcgaactca cgaaggccac catgtccggg gatccggatg tcgcgctcac catcggcttt
    13081 cggcaatcct ggttcgatga tccagcgcgg acgacagctg cgtcggcatg gatgctggca
    13141 gcctgcagaa tgggcgccga ctgcggagca gacagcaagg tctatccgtt tttcatgtgt
    13201 tacgacccga accaccccaa ttgcgacaag gatggaaacg tcgaactcat ggtgagcgct
    13261 tccctgagtc ccgagaagta cgccgatgcg tacgcccagt cgcaggtcat cgaagaggcg
    13321 ctgcggaacc gggattcagc agcgatcgag gcgctgctgc agaaattcgc gcgctagcgc
    13381 ctgatgctcg acggcagttc gacgaaatca ccggttctgt ccagcaggtt catggcggcc
    13441 agcagcgccg gcggcggcgc gatgagcttg cgcgccgcga gatccagcca gccaccggcg
    13501 ctcgtgacgc gcgcggcgag cttgccatcc gcgcggaaga tttcatggcg caacaggaaa
    13561 cggctgccat cgggcgaatg acccgcgagc gcatagttga cgtctatctt ctgctgcaac
    13621 ccgacctctt tgaagtactc gacttcatcc ttcatgacca ccgggccgat gcgcaggcgc
    13681 aggaattcct cgaccgggaa tccatgctcc atgaggaaca tctgccgcac gtcagcggcc
    13741 ttgtcgaggt aagccgtgtt tttcatgtgc gcgttgaagt ccatgtcgga ccagcccgcg
    13801 tacagcgtct tggtgaacat gggttgaact atacgaaaga atccggcgtc cggatgcgca
    13861 acgtaaaaag aatcccgcgc gcaccaggag ctgcgcatcc aggcgacata tcgccgcgta
    13921 acgtcccgtg aacgatacac accggcggct gttgccaccg aagccagggg accctcatgc
    13981 gcgcattcaa cacgtggttt gccatcgcct tcgcggcgct cgcatcttct ctctttaccg
    14041 gctgtgacga cgacaagaag gcggtagctc cttccatcac cgcccccgcc tcaccactcg
    14101 gcaatggtgt ggtcggttcg gcgatctcgc ccgtggattt cgtcacgagt ggcaccgtac
    14161 cgatcaccta cagcgtctcg gccggcattc tgccgccagg actcgcgctc gatccgacca
    14221 cgggcaggct gtccggctcg ccgacactcg tcggcatatt cgagttcacc atcaccgcca
    14281 cgaatcgcgc cggcacggac gatgcgcagt acatgcagac catcctgcgg ctgcccacga
    14341 tcaccacgcc cacctcaccg ctcgccgcgg ccttgggcgg cgctccattt acttcgctga
    14401 ccttcgaagc cagcggctcg atgcccatca cctggagtgt caccgcgggg acattgccgc
    14461 cgggcatgtc gctgaacgcg gccacgggtg cgtactccgg aactcccacc gccgcgggcg
    14521 attactcgtt cacggtgacg gcgaccaacg cagccggcag cgacagcgac gcattcacgc
    14581 aaaaggtcac ggccccggcc gcgaacgcgc acgtgctgat caatggcaac agtattgccg
    14641 ccgtcgcgac caccttcccg tcggggctcg acacgccgtt gtccctgacg ggactcaacg
    14701 cgggcgacgc actggtcagc atcgatcgtc ggccgcagaa cggattcctc tacgggctcg
    14761 gctacaacgc cgccgcgggc tccgtgcagt tgtattcaat ttcctcgacc acgggtgttg
    14821 cagcgccact gggcgcca
//
LOCUS       DASQJS010000112         4073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_687120, whole genome shotgun sequence.
ACCESSION   DASQJS010000112 DASQJS010000000
VERSION     DASQJS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687120"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VEW08_04510"
     CDS             <1..314
                     /locus_tag="VEW08_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HYJ40029.1"
                     /translation="NGDPLFDEDNNPVYEKVGGKHLFAGSVELIRDVGSKFAVAVFAD
                     AGNAFDSFDDPLMYSVGVGVRFRLPVVSVGIDVAQALTTPAGSNEPPGPRLHLNFSPK
                     L"
     gene            311..4063
                     /locus_tag="VEW08_04515"
     CDS             311..4063
                     /locus_tag="VEW08_04515"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HYJ40030.1"
                     /translation="MIRRRLKIAALIFGLLLALVLGFLAWVLYTEAGLRFAVARLPER
                     MGKVTVRIERVYGTIAGGFGADLADIDSERSHVRVEKGTARVNILPLLVGRIAVRAAG
                     AELVEIEVKRRLHPPPDVPPKFLPRFLSISAETARSRLLVIIAPNGRRVEFNDVTGSG
                     IVGNKTIRIFDSNVTFGVLQARSIGELTAADPMKLSGETTTRMIIEGQPTWRADSSFE
                     GTLDKLPLTLKLFEPFRADMRGELLSLSKDFHWTGKAEVHNFDLQAFGGGSALGIITG
                     PLDVGGEMNEFHARGPLMVPGLGSGPFDIVFEGNYADHVVNATHYEVTHRATGSRVDG
                     QGTIEPAGNGPQLLLYGNWHALRWPLVARYTAETPQIFSSPGGKYHLEGLWPYAITAS
                     GDLFVPQLDPMTVAVRGALHKDHLQMDELVLGAFGGKGNLAGEARWSPEESWSLAGSV
                     KGLNPGSLRPGFNGSLDFDMKASGAPFGGDGTLDFAFSDLSGKLRGNSATGSGRIQLQ
                     GENWTFDKLRFRAGSTRLAIDGELGASRALNLDFSLDADNLALLAEGARGELHASGRV
                     GGTSDAPIVKLTARGSEIEHGTMSVTKFAANIDVDWRGQRNSHADIAISELKAGERSL
                     TQFNATLDGTTSEHTVRADALAGITSLHLSGKGGFADGVWNGTIGDLFIDDTANINLQ
                     LDTPVKVRASAKDFKVNALCLHGKVARLCGEAAWNETGWTARADAHNLPISTLTAGLT
                     PNVEYQGTVNATASARSTGGAPPVGEARVDLVDAAIRHKLASGRTDVINFGSGFVTLK
                     AEGAQINGELRLDAAQRGLISGRVHADRVTTDVMDSPMRGQLQMSTGELSFLTLYFPA
                     VDRASGHFDANVSFDGTLGAPRASGIVKLSGAELDLYQLNLALRAVEMEARIVSNTLE
                     FSSTAKAGAGTLASSGKVEWREGLPYGEIRIDGENLRIVDVPEARIDASPDLDFRIAG
                     REISVKGEVKVPLARLQPADLTNAVLPSADERLVGPTEKVEEDPFLVTSEITMTLGDK
                     VTIDTYGLVGHITGSITERSLPGEPTRATGELQVRDGTYTALARKLDIERGKLIFNGG
                     LLADPGVDIRAIKVFPDVKAGVNVRGSLRAPRLSFFSEPTIPQAQIVSLLLAGGSLQS
                     VQDQSRVGAPAAQQEVAAQAGAFLAAQLGNKLGLPDISVESTLSNETSLVLGKYLSPR
                     LYVSYGVSLTESINTFKMRYTISDRWTIKTEAGKEIGADLVYTVEK"
BASE COUNT          775 a         1332 c         1271 g          695 t
ORIGIN      
        1 ccaacggtga tccgctcttc gacgaagaca acaacccggt ctacgaaaag gtcggcggca
       61 aacacctgtt cgcgggctcg gtggaactca tacgcgacgt cggctcgaag tttgccgtgg
      121 cggtgtttgc cgacgctggc aacgcgttcg acagcttcga cgacccattg atgtattccg
      181 taggtgtggg cgtgcgattt cgccttccgg tggtttccgt gggtatcgat gtcgcgcagg
      241 cgctgaccac gcccgcgggc agcaatgagc cacccggccc gcgcctccac ctcaatttct
      301 cgcccaaatt gtgatcaggc ggcgactcaa gattgcggcc ctcatcttcg ggttgctgtt
      361 ggccctggta ctgggattcc tcgcctgggt gctctacacc gaggcaggat tgcgtttcgc
      421 cgtcgcgcgc ttgcccgaac gcatgggcaa ggtcaccgtc cgcatcgaac gcgtttacgg
      481 gactatcgcc ggaggtttcg gcgccgacct ggccgacatc gacagcgaac gctctcacgt
      541 gcgcgtcgag aaaggcacgg cgcgcgtcaa tattctgccg ctgctggtgg ggcgaatcgc
      601 cgtgcgtgcc gcgggcgccg agctcgtgga gatcgaggtc aagcgccggc tgcacccgcc
      661 accggatgtg ccaccgaagt ttctgccccg gttcctgagc atcagcgccg agacggcccg
      721 ctcgcgcctg ctcgtcatca tcgcgccgaa tggcaggcgc gtggaattca acgacgtgac
      781 cggttccggc atcgtcggca acaagaccat ccgcattttc gacagcaacg tcaccttcgg
      841 cgtgctgcag gcgcgctcca tcggcgagct gaccgcggcc gaccccatga agctcagcgg
      901 tgaaaccacc acgcgcatga tcatcgaagg tcagcccacc tggcgcgccg attcgagctt
      961 cgagggcacg ctcgacaaac taccgctcac gctcaagttg ttcgagccgt ttcgggccga
     1021 catgcggggc gaactgctgt cactgtccaa ggacttccac tggaccggca aggcggaagt
     1081 ccacaacttc gacctgcagg cattcggtgg cggcagcgcg ctgggcatca tcacgggtcc
     1141 gctcgatgtg ggcggtgaaa tgaacgaatt ccacgcccgc ggtccgttga tggttccggg
     1201 tctcggcagc ggtccgttcg acatcgtgtt cgaaggcaac tatgcggatc acgtggtgaa
     1261 cgcaacccac tacgaggtca cgcacagggc gaccggcagc cgcgtcgacg gccagggaac
     1321 catcgaaccc gccgggaacg gcccgcaact ccttctctat gggaactggc acgcgcttcg
     1381 ctggccgctc gtggcgcgtt acaccgcaga gacaccgcag attttttcca gtcccggggg
     1441 caagtaccac ctcgaggggc tttggccgta tgccatcacc gcaagcggcg atcttttcgt
     1501 tccgcagctc gatccaatga cagtcgcggt gcgcggcgcc ctgcacaaag atcatcttca
     1561 gatggacgag cttgtgctcg gcgcattcgg tggcaagggt aaccttgccg gcgaggcgcg
     1621 ctggagcccc gaggagagct ggtcgctcgc ggggagcgtg aaaggactga atcccggcag
     1681 cctgcggcca gggttcaatg gatcactcga cttcgacatg aaagcgagcg gcgcgccttt
     1741 cggcggtgac ggaacgctcg atttcgcgtt cagcgatctg tccggaaaac tccgcggtaa
     1801 ctctgccacc ggcagtgggc gcattcagct gcagggcgag aactggacat tcgacaaact
     1861 gcgttttcgc gccggcagca cgcggttggc tatcgacggc gaactcggtg catcgcgcgc
     1921 cctcaatctc gatttcagcc tcgacgccga caatcttgca ttgctggccg agggtgcacg
     1981 cggcgagttg cacgccagtg ggcgcgtcgg cggcacgtcc gatgccccca tcgtcaaact
     2041 cacagcgcgt ggcagcgaaa tcgagcacgg cacgatgagc gtcaccaagt tcgccgccaa
     2101 tatcgacgtc gattggcgtg gccagcgcaa ctcccacgcg gacatcgcca tcagtgaatt
     2161 gaaagccggc gagcgatccc tgacccagtt caacgccacc ctcgatggaa cgacctcgga
     2221 gcacaccgta agggcggatg cgctggccgg catcaccagc ctgcatctgt ctggcaaagg
     2281 cgggtttgcc gacggcgtct ggaatgggac catcggcgat ctgttcatcg acgacaccgc
     2341 caacatcaat ctgcagctcg atactccggt gaaagtccgt gccagtgcaa aggacttcaa
     2401 ggtgaatgcg ctgtgcttgc acggcaaggt ggcgcggctc tgcggcgagg ccgcgtggaa
     2461 cgagacgggg tggaccgcgc gcgccgatgc gcacaacctg ccgatcagca cgctgaccgc
     2521 cgggctcacg cccaacgtcg aatatcaggg aaccgtgaac gccaccgcca gcgcgaggtc
     2581 gaccggtggc gcgccacccg tcggcgaggc acgcgtcgac ctcgttgacg ccgccatcag
     2641 gcacaagctc gcgagcggcc gcaccgatgt catcaatttc ggttccggct tcgtgacact
     2701 gaaagccgaa ggagcgcaga tcaacggcga gttgcggctc gacgccgcac aacgcggcct
     2761 gatatccggc cgggtgcacg ccgaccgtgt caccaccgac gtcatggatt cgcccatgcg
     2821 cgggcagctg cagatgtcga ccggcgagct cagcttcctg acgctgtact ttcccgccgt
     2881 ggatcgcgcg agcggtcact tcgatgccaa cgtctcgttc gacggcacgc tcggtgcgcc
     2941 gcgcgccagc ggcatcgtca aactatccgg agcagaactc gacctgtacc agctcaatct
     3001 ggcgctgcgt gcggtcgaga tggaagcgcg catcgtatcc aacacgctcg agttcagctc
     3061 caccgccaaa gcgggcgccg gcacgctggc gagctcgggc aaggtcgagt ggcgtgaagg
     3121 tcttccgtac ggcgaaatcc gcatcgacgg cgaaaacctg cgcatcgtcg acgtgccgga
     3181 ggcgcgcatc gacgcttctc cggatctcga tttccgcatt gccggtcgcg agatctcggt
     3241 gaagggcgag gtcaaggtcc cgctcgcgcg cctgcaaccc gccgatctca ccaacgccgt
     3301 gctgccatcc gccgatgaaa ggctggtggg gcccacggaa aaggtcgaag aagatccgtt
     3361 cctcgtcacc agcgaaatca ccatgacact tggcgacaag gtgaccatcg acacgtacgg
     3421 cctggtggga cacatcacgg gaagcatcac cgagcgaagc ctgccaggcg agccgacgcg
     3481 cgctaccggt gaattgcagg tcagagacgg cacttacacc gcgttggcgc gcaagctcga
     3541 catcgagcgc ggaaagctga tcttcaatgg cgggttgtta gccgacccgg gcgtcgacat
     3601 ccgtgcgatc aaggtattcc ccgacgtgaa ggcaggcgta aacgtgcgcg gcagcctgcg
     3661 cgcaccgcgg ctctcgtttt tctccgaacc caccatcccg caggcgcaga tcgtctcgct
     3721 gctgctcgca ggcggctcgc tccagtcggt gcaggaccag agccgcgtcg gcgcccccgc
     3781 cgcgcagcag gaagtggccg cccaggcggg cgcatttctc gccgcgcagc tcggcaacaa
     3841 gctcggactt cccgatatca gcgtcgaatc gacgctgtcg aacgaaactt cgctggtgct
     3901 cggcaaatac ctgtcgccac gactgtacgt tagttatggc gtctctctca ccgagtccat
     3961 caacacgttc aagatgcgat acacgatcag cgatcgctgg accatcaaga ccgaggcggg
     4021 caaggagatc ggcgccgacc tcgtctacac cgtcgaaaag tgaacacgct cga
//
LOCUS       DASQJS010000113         3599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3206403, whole genome shotgun sequence.
ACCESSION   DASQJS010000113 DASQJS010000000
VERSION     DASQJS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3206403"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="VEW08_04520"
     CDS             <1..1070
                     /locus_tag="VEW08_04520"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HYJ40031.1"
                     /translation="NGFGRGADNLAAVESLTIYRAFRYGRNAELILTDNRSYKIADAD
                     GSAVSPDGFPMFIDEDTARIIDDGRAANGGKPPATLRFADHEVPNAGVDQPMQTYLGP
                     KQRAWLIDRLSVSQAPWKVWGHSFGTLAWRTDLANLPEELGPKWPGTGFGLANGGYYS
                     EHEQIYDVLRKKGVTGLAVVAGDKHSFWAGLTSADLPPRAYDPVGVEFVTGSISAQGL
                     VEIGDRIVKPDHALRALYYTGERREIPAINYTLLHGVRAAVALSRTSAAEARKLHNPA
                     VAPHLKFVDLGGHGYGLVTVTGDWLETEFVSIPVPFERSDSADGGPLRYRVRHRVNRW
                     KAGEQPKLAQTVVEGNADYSI"
     gene            1180..1494
                     /locus_tag="VEW08_04525"
     CDS             1180..1494
                     /locus_tag="VEW08_04525"
                     /inference="COORDINATES: protein motif:HMM:NF025077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HYJ40032.1"
                     /translation="MLQKAFAAFVSVLLVSCASLSDAGREVMVVGSEAAVGNCRLLGH
                     VSARTGLRGVASLIAADEQLDTKLRNKVARKGGNYLLLELKDTFLDEESYAYGKAYAC
                     AK"
     gene            1633..2088
                     /locus_tag="VEW08_04530"
     CDS             1633..2088
                     /locus_tag="VEW08_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HYJ40033.1"
                     /translation="MKNKVSSARALAIAFCCMSAAQASPVPADEEAVAQALRLMYVAL
                     TKEDSAQLRAVTASDFYAFDGGEKMTGDELMSIIKSAHAAGKTYVWTVPEPKVRVEGA
                     VAWITYLNRGSVQDAAGKRDVSWLESAVLLKEAGTWRIQFFHSTRVPAK"
     gene            2102..3568
                     /locus_tag="VEW08_04535"
     CDS             2102..3568
                     /locus_tag="VEW08_04535"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HYJ40034.1"
                     /translation="MRLGFQNTYSALPSRFYARVAPAPVKDPRLVVFNSRLAEELDFD
                     PAVLEHEGAAWFSGNQTPEDADPIALAYAGHQFGSFVPQLGDGRAILLGERVVRGVRR
                     DIQLKGSGRTPFSRNGDGRAALGPMLREYLISEAMHALGIPTTRSLAVVTTGEQVVRE
                     DLQPGAILTRVAASHIRVGTFEYFAARNDRDAVSQLLDYVITRHVPDARDADVPALAV
                     LEAVVQRQAALIADWMSVGFIHGVMNTDNMAISGETIDYGPCAFMDHYAANTVFSSID
                     HAGRYAYGNQPAIAQWNLARFAETLLPLVDSDMGKAVEIATAVVKDFMPRFDAQLLTR
                     MRLKIGLASQQEGDVELVRSLLAAMQSAGADFTLTFRRLARCADEPADDASLLELFAS
                     SPDIADWLRRWRERLASEPQNAAERAANMRHVNPAFIPRNHRVEAALEAASTHGDLGP
                     FHQLLKILEHPYDDQPGDEEFELPPAPGERVLRTYCGT"
BASE COUNT          647 a         1148 c         1176 g          628 t
ORIGIN      
        1 agaacggctt tggccgcggc gccgacaacc tggcggccgt cgagagcctc accatctacc
       61 gcgcgttccg ttatggccgc aacgccgagc tgatcctcac cgacaaccgc tcatacaaga
      121 tcgccgacgc ggacggttcc gcggtctcac ctgacggatt cccgatgttc atcgacgagg
      181 acacggcgcg catcatcgat gacggccgcg ccgcgaacgg cggcaagccg cccgcgacac
      241 tgcggttcgc agaccatgaa gttccgaacg caggcgtcga ccagccgatg cagacctacc
      301 tcgggccgaa gcagcgcgcg tggctcatcg atcgcttaag cgtgagccag gcgccgtgga
      361 aggtgtgggg acactcgttc ggcacgctcg cgtggcgtac ggatctcgcc aacctgcccg
      421 aggagttagg ccccaagtgg ccgggcacgg gcttcgggct ggcgaacggc ggctactact
      481 ccgagcacga gcagatctac gacgtgttgc gcaagaaggg agttaccggg ctcgcggtgg
      541 tggccggcga caagcactcg ttctgggcag gcctgacttc cgcggatctg ccgccgcgcg
      601 cttacgatcc ggtgggagtc gagttcgtca cggggtccat ctcggcgcag ggcctcgtcg
      661 agattggcga ccgcatcgtg aagcccgacc acgcgctgcg cgcgctgtac tacaccggcg
      721 agcgccgcga aatccccgcg atcaactaca cgctgctgca cggcgtgcgc gccgcggtgg
      781 cgctgtcacg cacgagcgcc gccgaggcgc ggaagcttca caacccggcc gtcgcgccgc
      841 atctcaaatt cgtggatctt ggcggacacg ggtacggcct ggtcaccgtg accggcgact
      901 ggttagaaac cgagttcgtc agcatcccgg tgccgttcga gcgcagcgac tcggcggatg
      961 gtgggccgtt gcgctaccgc gtgcggcatc gtgtgaaccg gtggaaggcg ggtgagcagc
     1021 cgaagctggc gcagacggtc gtcgagggga atgcggacta ctcgatctga cttcgagcgc
     1081 cccggtcgtg caacgtcact gcatccgaac gccgcgggca cccgtactaa aggcgcgcaa
     1141 tgaacgcctt tcggggcaac gacagcgagg gacccttcca tgttgcagaa ggcatttgcc
     1201 gcattcgtct cagtgctgct cgtgtcctgc gcgtctctgt ctgacgctgg ccgggaagtg
     1261 atggtcgtcg gttcggaagc ggcggtgggc aactgcaggc ttctcgggca tgtcagcgcc
     1321 cgaacggggc tcagaggcgt cgcgagcttg atagccgcag acgagcagct cgatacgaaa
     1381 ctgcgcaaca aggtcgcgcg caagggcggc aactacctgc tgctcgaatt gaaggacaca
     1441 tttctggacg aagaatccta tgcctacggc aaggcatacg cctgcgcgaa gtagcacggc
     1501 ggctggtggg ccgttgcgat atcgcgagcg gcaccgcgtg aaccggtgga gcagcctaag
     1561 ctcgtgcaga ccgtcgtcga ggggaatgcg gactactcga tcttggttac aggagcctgt
     1621 aagttggccg ccatgaaaaa caaagtgtct tcggcccggg ctctcgccat cgcattctgc
     1681 tgcatgagtg cagcgcaagc ctcgccggta cccgccgatg aagaggccgt ggcgcaggcg
     1741 cttcggctga tgtacgtcgc cttgaccaaa gaggactccg cccagctccg agccgtcacc
     1801 gcttccgact tctatgcgtt cgatgggggc gagaagatga ctggcgatga actcatgtcg
     1861 atcatcaaga gcgcgcatgc ggccggcaag acctatgtgt ggactgtccc ggagcccaag
     1921 gtgcgtgtcg aaggggccgt ggcgtggatc acttacctga atcgcggctc tgtgcaggat
     1981 gccgcgggga aaagggacgt gagctggctg gagtcggcgg ttctgctcaa ggaagcaggc
     2041 acctggcgca tccagttttt ccacagcacg cgggtgcccg cgaagtagat ccccgccggt
     2101 catgcggctc ggtttccaaa acacttattc cgcgctgcct tcgcggttct acgcgcgggt
     2161 cgcgccggcg cctgtgaagg atccacgact ggtcgttttc aactcccgtc tggccgaaga
     2221 gctcgacttc gatccagccg tcctcgaaca cgaaggggca gcctggtttt caggcaatca
     2281 aacgcccgaa gatgccgacc cgatcgcctt ggcctacgcg ggtcatcagt tcggcagctt
     2341 cgtgccgcag ctcggtgatg gccgcgccat cttgctgggt gaacgcgtgg tgcgcggcgt
     2401 gcggcgcgac atccagctca agggctcggg acgcacgccg ttctcgcgca atggcgacgg
     2461 acgcgcggcg ctcgggccca tgctgcgcga gtacctcatc agcgaagcga tgcatgcgct
     2521 cggcattcca accacgcgct ctctcgcggt ggtcacgacc ggcgagcagg ttgtgcgcga
     2581 ggatcttcag cccggcgcca tcctcacgcg cgtggcggcc agtcacatcc gtgtcggcac
     2641 cttcgagtac ttcgcggctc gcaacgatcg cgatgcagtg agtcagctgc tcgactacgt
     2701 catcacgcga cacgttccag acgcgcggga cgccgacgtt cccgcgctgg cggtgctcga
     2761 agccgtggtg cagcgccagg ccgcgctcat tgcggactgg atgagcgtgg gcttcatcca
     2821 tggcgtcatg aacacggaca acatggccat ctcgggtgag accatcgact acggaccgtg
     2881 cgcattcatg gatcactatg ctgcgaacac cgttttcagc tccatcgatc atgccggtcg
     2941 gtatgcgtac ggcaatcagc ccgccattgc gcaatggaat ctcgcccggt tcgccgaaac
     3001 cctgctgccg ctcgtcgact cggacatggg gaaagcagtc gagattgcga cagccgtggt
     3061 caaggacttc atgccgcgtt tcgatgcgca gctcctcacg cgcatgcggc tcaagatcgg
     3121 tcttgcgagc cagcaggaag gcgatgtcga gctcgtcagg agtctgttgg cagcgatgca
     3181 atcggccggt gcggatttca cgctgacgtt tcgtcgcctc gcgcggtgtg ccgacgaacc
     3241 ggccgatgac gcctcactac tcgagctgtt cgcatcctcc cctgacatcg ccgattggct
     3301 gcggcgctgg cgcgagcgcc tcgcaagcga gccgcaaaat gccgcggagc gcgccgcgaa
     3361 catgcgccac gtgaatcccg cattcattcc gcgcaaccac cgggtggagg ccgcgctcga
     3421 agcagcctcc acgcacgggg acttaggtcc ctttcaccag ctcctgaaaa tcctcgagca
     3481 tccttacgat gatcagcctg gcgacgagga gttcgagctg ccgccggcgc cgggcgaacg
     3541 cgtgctacgg acgtactgcg gcacttgagt ttcagttcag gacgtgcgcg tgaaaccag
//
LOCUS       DASQJS010000114         6201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_372598, whole genome shotgun sequence.
ACCESSION   DASQJS010000114 DASQJS010000000
VERSION     DASQJS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372598"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..144
                     /locus_tag="VEW08_04540"
     tRNA            70..144
                     /locus_tag="VEW08_04540"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:102..104,aa:Gln,seq:ttg)
     gene            202..1140
                     /locus_tag="VEW08_04545"
     CDS             202..1140
                     /locus_tag="VEW08_04545"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HYJ40035.1"
                     /translation="MALFAGNANPALAHDIARHLTTPLGRAYVGRFSDGEVNIELMEN
                     VRGRDVFILQPTCPPANDHLMELLVIVDACRRASAAKITAVVPYFGYSRQDRRPRATR
                     SAITAKLVANMIQSAGVDRLLTIDLHSDQIQGFFDIPVDNVYASPVLLGDAYRQRYEN
                     MIVVSPDVGGVVRARALAKRLDDADLAIIDKRRPRPNESKVMNIIGEVRGKTCVLIDD
                     MVDTAGTLAAGAQALKDEGAVKVVAYITHAILSGNAIEKISKSAIDELVVTDTIPLST
                     AARQCGRIRQLSVAGLLAETIRRIRDEDSVSSLYLD"
     gene            1324..2073
                     /locus_tag="VEW08_04550"
     CDS             1324..2073
                     /locus_tag="VEW08_04550"
                     /inference="COORDINATES: protein motif:HMM:NF004128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HYJ40036.1"
                     /translation="MRISFELAAEFRDAEGKGASRRLRHAGRVPAILYGGHKEPRAIA
                     LNHQKLMTLVDNEKFYSSIINLVVDDKKQAAIVKDLQMHPARNAIVHVDLQRVVETEK
                     IRIHIPIHFKGEAASPGVKSQGGVVSHRIADVEISCLPKDLPEFIELDLSQMEMNHSK
                     HLSDLPLPEGVTIPGIAKADAVVVTIHPPRAEEPEPTAEVAAATPAEGAAAAPAEGAA
                     AAPGAKAGDAKAGDAKGAAAAAPAKKEGGKK"
     gene            2185..2793
                     /gene="pth"
                     /locus_tag="VEW08_04555"
     CDS             2185..2793
                     /gene="pth"
                     /locus_tag="VEW08_04555"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HYJ40037.1"
                     /translation="MSGNVLKLIVGLGNPGPEYARTRHNAGFWLVDELARRHGGVFRF
                     EGKHQAELARIRIGGEELWLAKPMTFMNRSGGPVSSVLGFYRITPEQMLVAHDEIALP
                     SGTVRLKEAGGHGGHNGLRDLIAAQGDGFWRLRIGVSHPGTKNEVVDFVLTRAGADEQ
                     RVIDETIVAGADAIEQMLRSGAQIAMNKLHSLGKPAANAKPE"
     gene            2798..3889
                     /gene="ychF"
                     /locus_tag="VEW08_04560"
     CDS             2798..3889
                     /gene="ychF"
                     /locus_tag="VEW08_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HYJ40038.1"
                     /translation="MGIKCGIVGLPNVGKSTLFNALTRAQIAAENYPFCTIDPNVGIV
                     PVPDPRLDALSKIVEPEKIVATTVEFVDIAGLVAGASQGEGLGNKFLAHIRETDAISH
                     VVRCFEDADIVHVSGRVNPISDIDIIDTELALTDLQSVEKAVDRNTKAAKGGDKDAAR
                     KKDLFERMRKHLDDGKPVRSMGLTKEEKADSQELHLLTAKPVMYVANVKEDGFTNNAH
                     LDAVTNRAKSEGAVVVAVCAAIEAEISQLDEADRADFLRDLGLAEPGLNRVIRAGYEL
                     LGLQTYFTAGVKEVRAWTVRGGSTAPQAAGVIHTDFERGFIRAEVIAYDDFIKYQGEA
                     GAKDAGKLRLEGKEYLVKEGDVMHFRFNV"
     gene            3923..5380
                     /locus_tag="VEW08_04565"
     CDS             3923..5380
                     /locus_tag="VEW08_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ40039.1"
                     /translation="MLARVRKILRRIGIAIGIVIATILVVRSFDAWRSPPLKLWHTQV
                     PHELDADEIDAADWESWVVAENAAFAEVRQEVIDALPADDRIPINRYFAASPINPLGY
                     AQNWNHSFVLAPIGTPRGAVVLLHGLTDSPYSMRHIALRYVERGYVAVAVRMPGHGTV
                     PAGLTTTRWEAWLAATRLAVRTARAMAGDKVSLHVVGYSNGGALALKYALDSLSDPKL
                     ARPDRVVLLSPMVGITSVARFAGVLGWPAAFPAFAKSAWLDVLPEYNPFKYNSFPVNA
                     ARQSSQVVGALQEEFARAAKSGVLAKLPPVLTFQSVLDATVSTSAIVDSLYRNLPANG
                     SELVLFDVNRRAGVGPIIRPEQANVLATLLPPAPRQFAVTVVGNGAEDASTDEARTVT
                     AGATETRVTALGRLYPKDLYSLSHVALPFPLQDGLYGSEPGANEDFGVRLGMVAVRGE
                     RGALIVGAETLMRASSNPFFDYMLERIEAGIAYAE"
     gene            complement(5468..>6201)
                     /locus_tag="VEW08_04570"
     CDS             complement(5468..>6201)
                     /locus_tag="VEW08_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ40040.1"
                     /translation="PTRGIVPPDEFIPVAEESGLIVQLSDQVMRQALKQISEWWQAGC
                     RDLRVAVNVSGAQFEAPGFPDWVFAHLDEAGLPGSCLELEITESLLMADEAAAALAVR
                     AVRELGVHVAIDDFGTGYSSLAYLKHFPIEALKIDRSFVADLGRDSNDAAICAAIIAM
                     GRQLGLKIVAEGVETQEQLDFLATHGCTLAQGFFIARPVEAAEMAKMLRIGSNEAPML
                     PMPRKAKALEVPLADYEAAALLKVR"
BASE COUNT         1149 a         1898 c         2063 g         1091 t
ORIGIN      
        1 ggttgcacgc gggctggaca gttctccttt gctggaagaa ttggcccgct gctcgaacag
       61 ttaggccgtt ggggtgtcgc caagtggtaa ggcagcgggt tttgatcccg ccattcggag
      121 gttcgaatcc ttccacccca gccagttgga ttcaagaagg tttgctagtg ggagaggtag
      181 ctagtggcgg aatctgaaac gctggcgctg ttcgcgggta acgccaatcc ggcgctggct
      241 cacgacatcg cacgccacct gacgactccg ctcgggcgcg cctacgtcgg aaggttctcc
      301 gacggcgaag tgaacatcga gttgatggag aacgtgcgcg ggcgggatgt gttcatcctt
      361 cagcccacgt gtccgccggc caacgaccat ctgatggagt tgctggtcat cgtcgatgcc
      421 tgccggcgcg cgtcggcggc caagatcacg gcggtggtgc cgtacttcgg ctactcgcgc
      481 caggatcgcc ggccgcgcgc cacgcgctcg gcgatcaccg cgaagctggt ggcgaacatg
      541 attcagagcg cgggggtgga tcgtctgctg acgatcgacc tgcactcgga ccagatacag
      601 ggatttttcg acatcccggt ggacaacgtg tacgcctcgc cggtgttgtt aggcgatgcg
      661 taccggcagc gctacgagaa catgatcgtg gtgtcgccgg acgtcggtgg cgtggtgcgc
      721 gcacgggcgc tggcgaagcg gctcgacgac gcggacctgg cgatcatcga taaacgccgt
      781 ccgcggccga acgagtcgaa ggtgatgaac atcatcggtg aagtgcgcgg caagacctgc
      841 gtgctcatcg acgacatggt ggacaccgcg ggcacgctgg ccgccggtgc gcaggcattg
      901 aaggacgagg gcgccgtgaa ggtggtggca tacatcacgc acgccatcct ctccggcaat
      961 gcgatcgaga agatttcgaa gtcggcgatc gatgagctgg tggtgaccga caccatcccg
     1021 ctgtcgacgg cggcgaggca atgtggacgt atccggcagc tgtcggttgc gggcctgctg
     1081 gccgagacca tccgtcgcat ccgcgacgag gattctgtca gcagcttgta cctcgattag
     1141 ttggcgggtc ggtccccatt tcccggaaaa aggggccgga ccgcgaagtg tttgtttggt
     1201 cgattagttg agtacggatt gaggtccggc ccctttttcc aggaaatggg gactgacccc
     1261 attcttaggt ggcggcactg gtcgcggtgt ccgccgaata ttgatgtaac tggagtttca
     1321 atcatgcgta tttcattcga actcgccgcc gagttccgcg atgcggaggg caaaggtgcg
     1381 agccgccgcc ttcgtcacgc gggacgagtg ccggcaatcc tgtacggggg ccacaaggaa
     1441 ccccgtgcga tcgctctcaa tcaccagaag ctgatgacgc tggtggacaa cgagaagttc
     1501 tattcctcga tcatcaatct cgtcgtcgac gacaagaagc aggctgcgat cgtcaaggat
     1561 ctgcagatgc acccggcgcg caacgccatc gtgcacgtcg acctgcagcg cgtcgtggag
     1621 accgagaaga tccgcatcca catcccgatc cacttcaagg gtgaggccgc gtccccgggc
     1681 gtgaagagcc agggcggcgt cgtgtcgcac cgcatcgccg acgtggaaat ctcctgtttg
     1741 ccgaaggacc tgcccgagtt catcgagctc gacctctcgc agatggagat gaaccactcc
     1801 aagcacttgt ccgatctgcc gttgccggaa ggcgtcacga tccccggaat cgccaaggcc
     1861 gatgccgtgg tcgtcacgat ccatccgccg cgtgcggaag agccggaacc gaccgccgaa
     1921 gtcgccgctg caacgcctgc cgaaggcgcc gctgctgctc cggccgaagg tgcggccgct
     1981 gcaccgggtg cgaaggctgg cgatgccaag gccggcgacg ccaagggcgc cgctgccgcg
     2041 gctcccgcga agaaggaagg cggcaagaag taagcatgtg ttggggacgg gccatcgggc
     2101 ccgtcctccg ctcacaaact gtacttacgc ggcgcgccga tcactcggcg cgccgcgatt
     2161 gtttcaggtc ctggcaaact cacgatgtcc ggtaacgtac tcaaactcat cgtcggcctc
     2221 ggcaatccgg gtcccgaata tgcgcgcacg cgtcacaacg cgggcttctg gctcgtggat
     2281 gaactggcgc ggcgtcacgg cggcgtgttc cgtttcgaag gcaaacacca ggcggagctc
     2341 gcgcgaattc gcatcggcgg cgaggagcta tggctcgcaa aaccgatgac cttcatgaat
     2401 cgaagcggcg gaccggtgtc gagcgtgctg ggcttctaca ggatcacccc ggaacagatg
     2461 ctggtggcgc acgatgaaat cgccctgccg agcggcacag tgcggctcaa ggaagccggc
     2521 ggccacggtg gacacaacgg cctgcgcgac ctcatcgcag cgcagggcga cggcttctgg
     2581 aggctgcgca tcggcgtgag ccatccggga acgaaaaacg aagtcgtgga cttcgtgctg
     2641 acgcgcgctg gcgcggacga gcaacgcgtg atcgacgaga ccatcgtcgc cggcgcggat
     2701 gcgatagaac agatgctgcg ctcgggagcg cagatcgcca tgaacaaact gcattcgctc
     2761 ggcaagcccg ctgcgaacgc gaaaccggag tagatggatg ggtatcaagt gcggcatcgt
     2821 gggtctcccc aacgtcggca agtccacgct gttcaatgcg ttgacgcgcg cgcagatcgc
     2881 ggcggagaac tacccgttct gcaccatcga tccgaacgtc ggcatcgtgc ccgtgccgga
     2941 tccgcgactc gacgcgctgt cgaagatcgt ggagcccgaa aagatcgtcg ccaccacggt
     3001 cgagttcgtg gacatcgccg gcctggtggc tggcgcgtcg cagggcgaag gcctcggcaa
     3061 caagttcctg gcccatatcc gtgagaccga cgcgatttcg cacgtggtgc gctgcttcga
     3121 ggatgccgac atcgtgcacg tgtcgggccg ggtgaatccc atttcggaca tcgacatcat
     3181 cgacacggaa ctcgcactca cggacctgca atccgtggag aaggcggtgg accgcaatac
     3241 aaaagccgcc aagggtggcg acaaggacgc cgcgcgcaag aaagacctgt tcgagcgcat
     3301 gcgcaagcac ctggatgacg gcaagcccgt gcgctccatg gggctcacca aggaagagaa
     3361 agccgacagc caggagctgc acctgctcac ggcgaagccc gtgatgtacg tggccaacgt
     3421 gaaggaagac ggcttcacca acaacgcgca tctcgatgcg gtgaccaatc gcgcgaagtc
     3481 cgaaggtgca gtcgtcgtcg cagtctgcgc ggccatcgaa gcggagatca gccagctcga
     3541 tgaagccgat cgcgccgact tcctgagaga cctcgggctt gccgagcccg gcctcaaccg
     3601 cgtgatccgc gccggctacg agctgttagg cctgcagacc tatttcacgg cgggcgtgaa
     3661 ggaagtgcgc gcctggacgg tacgcggcgg ctctaccgca ccccaggcgg ccggcgtgat
     3721 ccacacggat ttcgagcgcg ggttcattcg cgccgaagtc atcgcctacg atgacttcat
     3781 caagtaccag ggcgaggcgg gcgcgaagga tgccggcaag ctgcgcctcg agggcaagga
     3841 atacctggtg aaggaaggcg acgtcatgca cttccgtttc aacgtctagt tcgcggggca
     3901 cggccccgac gaggtggtga tcatgctcgc cagggttcga aagatcttgc gccggatcgg
     3961 catcgcgatc ggcatcgtga ttgccaccat tctcgtcgtg cggtcattcg atgcctggcg
     4021 ctcgccgccg ctcaagctct ggcacacgca ggtgccgcac gagctcgatg cggatgagat
     4081 cgacgccgcg gattgggaat cctgggtggt ggcggagaat gccgcgttcg ccgaggtgcg
     4141 gcaggaagtc atcgatgcct tgcccgcgga tgatcggatt cccatcaatc gatatttcgc
     4201 cgcgagcccg atcaatccgc tgggctacgc gcagaactgg aatcactcgt tcgtactcgc
     4261 gccgatcggc acgccgcgcg gcgccgtggt gctgctgcat gggttgaccg attcgccata
     4321 cagcatgcgg catatagcgc tgcgttatgt cgagcgcggc tacgtggccg tcgcggtgcg
     4381 catgccgggg cacggcaccg tgcccgccgg tctcacaacc acgcgctggg aagcgtggct
     4441 cgccgcgacc cggctcgcgg tgcgcacggc gcgcgcgatg gcgggcgaca aagtgtccct
     4501 gcacgtagtg ggctattcga acggcggcgc gctggcgctg aaatatgcac tcgattcgct
     4561 ttccgacccg aaactggcgc ggcccgaccg ggtggtgctg ctctcaccca tggtcggcat
     4621 cacgagtgtt gcgcgtttcg ccggcgtgct cggctggccg gccgcgtttc cagcgttcgc
     4681 caagtcggcg tggctcgacg tgctgccgga atacaacccg ttcaagtaca actcgtttcc
     4741 ggtgaacgcc gcgcggcaga gttcgcaggt ggtgggtgca ttgcaggaag agtttgcccg
     4801 cgccgcgaaa agcggcgtgc tcgccaaact accgccggtg cttactttcc agtcggtgct
     4861 cgatgccacg gtttcgacca gcgccatcgt ggattcgctg taccggaacc tgccggcaaa
     4921 tggcagcgag ctggtgctgt tcgatgtcaa tcgccgcgcg ggtgtggggc cgatcatccg
     4981 gcccgaacag gcgaatgtgc tggcaacgct gttgccgccg gcgccgcgcc agtttgcggt
     5041 cacggtggtg gggaacggcg cggaagatgc gtcgaccgat gaggcccgca ctgttacggc
     5101 aggcgcgacc gaaacgcggg tcacggcgtt gggccggctc tatcccaagg atttgtattc
     5161 actgtcgcat gtggcgctgc cgtttccgct gcaggatggg ttgtatggca gcgagccagg
     5221 cgcgaatgag gatttcggcg tgcggctggg catggtggcg gtgcgcggcg agcgcggcgc
     5281 gctgatcgtc ggcgccgaga cgctcatgcg cgcgagctca aacccgttct tcgactacat
     5341 gctggaacgc atcgaagcgg gaatcgcgta cgcggagtga ccctgagata aaaggggaca
     5401 gatctattta ttgaagaggt gttgcggtgc tcacgcaagt gccttcaata aatagatctg
     5461 tcccctttta tctgactttg aggagcgcgg cagcctcgta gtcagcgagc ggcacctcga
     5521 gcgccttcgc cttgcggggc atggggagca tcggtgcttc gttgctgccg atgcgcaaca
     5581 tcttcgccat ttcggccgct tcgaccggac gcgcgataaa gaagccctgc gccagtgtgc
     5641 agccgtgcgt ggccaggaaa tccagctgtt cctgggtctc cacgccttcc gcgacgatct
     5701 tcaaccctaa ctgccggccc atcgcgatga tggcggcgca gatcgccgcg tcgttggaat
     5761 cgcggccgag atcggcgacg aacgagcggt cgatcttcag cgcttcgatg gggaagtgct
     5821 tcagataggc gagcgacgaa taacccgtgc cgaagtcatc gatggccacg tgcacgccga
     5881 gttctcgcac cgcgcgcacc gcgagcgccg cggcggcttc gtcggccatg agcaggctct
     5941 cggtgatctc gagctccagg catgaacccg gcaatcccgc ttcgtccagg tgcgcgaaca
     6001 cccagtccgg gaaacccggc gcctcgaact gcgcgcccga gacgttgacg gccacgcgca
     6061 agtcgcggca tcccgcctgc caccattcgg aaatctgctt caacgcctgg cgcatcacct
     6121 ggtcggagag ctgcacgatg aggccggact cttcggccac cgggatgaat tcatccggcg
     6181 gcacgatgcc gcgcgtggga t
//
LOCUS       DASQJS010000115        21669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4458019, whole genome shotgun sequence.
ACCESSION   DASQJS010000115 DASQJS010000000
VERSION     DASQJS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21669
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4458019"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VEW08_04575"
     CDS             complement(<1..357)
                     /locus_tag="VEW08_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40041.1"
                     /translation="MSATKLRGRERQETMDNVSLNIESEYSGPTRSFKTQNSSQLNVK
                     QFARRDLFFFEPENLFEVAAERAREPECELERRRIFFRFERYDRLPCDAAEARKFRLR
                     HRGIFLPPAADFIAYRG"
     gene            356..742
                     /locus_tag="VEW08_04580"
     CDS             356..742
                     /locus_tag="VEW08_04580"
                     /inference="COORDINATES: protein motif:HMM:NF018552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HYJ40042.1"
                     /translation="MSLYESNYLRLAQLAGNLAALADMRVSRVPEDCDLRLTVCERSP
                     YTTSFALTYLFDPEGLTTYPDMRVRVYHDARLVEAQEWASQHEHEALRRLRGVAEREL
                     DQRWARNTMLNKWLEYCIDRGHGFRT"
     gene            772..1827
                     /locus_tag="VEW08_04585"
     CDS             772..1827
                     /locus_tag="VEW08_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40043.1"
                     /translation="MKALKTFGFLGGTNAAEPPPEDGRVVELFRSRAELRKQHEELGA
                     EMQRLRDRLKQQEAATGRVQELLESLEKRLEAADTGLSSLVFYHLRALWGDGREIIGL
                     LVQDLVAKQEERERKAWALEGNRKTFQSRQAAEQQLRQAESLMVAARQQLVAFDARLA
                     KLDKWWHRWQRPTVVAQRPALQAAVEAAEASLVGARARCEQLEKAGAGEFPGISVEAK
                     RAINLAAIACAEVLCLRLARTSLVSSARAAMARRESLEYYGDLAACVSIMSDIVKARN
                     LLKQRAGITQEVQQRSDRLRSTATYLGEFETVPIPESCGVSEGDVLAHGSQGVSSAKL
                     PNVVAEDTWDLFKVLLR"
     gene            complement(1808..4924)
                     /locus_tag="VEW08_04590"
     CDS             complement(1808..4924)
                     /locus_tag="VEW08_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYJ40044.1"
                     /translation="MVHTDFALRRPVTTLMAFAAVAVIGIVSARLLPLEQYPDVSFPF
                     MGAGVPYPGSTPEETEELITRPIEDALATLPGIEEIRSTSSDQDARFEIRFAWGTDIN
                     TAGFEVRNKLDSVRSSLPKTADRMWMWMASTADSPMLTVRFSADQDLSSQYDLLDRYF
                     KKPIERLDGVARVELAGVEAREVRILVDPGRLAAHAVDIRQLVLLLEKSNFSVAAGQI
                     TGNGERLSVRPLGEFRSLDEIRNLVIKGNVHLGDVASVELVSPEITRRRHLDGRPAVG
                     LDVFKSTQANVVDVVDRVLEVIEHNKALPQMQGIRVFTIDNQAEAIRSSLSDLTEAGL
                     IGAALAFTVLFLFLRHWPTTLIVSVSVPLSLLVTLAVMYFVGLTINVMSMMGMMLAIG
                     MLVDNAVVVTESVFRHRQMDPEHPQAATLAGVKEVGVATLAGTATCVVVFLPILFGSS
                     NQLSIWLTHAAIPICVAMVASLVIAQTLIPMVTSRFPAPPPLDSRSWISRLQDRYTRT
                     LDWSIHHRGWTAFGLFAVLALTVGLIVLSNIFPGKFIKFDPGAQDGGGQVFLGYNIKG
                     SHPIDRVEAAVETIERHFDEKRREELGIKSVYSVYDQSSAFSILVLKPRDEGGPKSQE
                     LIEKAQAGLPEIIIGKPSFKWDDENSMGGQRFSVQLTGESTEKLAELGDEVARVMSSV
                     KGLEAVRSEAREGDEEVQIIVNRQRAAALGLTSSDVAQAVAAGMRGDRLREFRGTERE
                     LTLRLAFRESDRQTVDNLAEFPIYLPTGTRVPLSAVADFRIARGPRAIERVDRLTSVA
                     ITGNVKKESTLDAVGKEVEALMKNYQLPPGYTWKLGKGFDQQDENSQTMLFNLLLAIA
                     MIYLVMAAVFESTVYPMSIITSIFMAIIGVIWLLFVTRTTITLMAFIGVQILIGVVVN
                     IGIVLVAHINELRSTGMERMTAIVQAGRDRLRPILMTTLTASLGLLPLAAGDASLAVG
                     GGGPSYAPMARSIMGGLLAGAVMSLFVVPAFYVWIDNGAERVRRFMRRTRVVDAPPSP
                     AGISEAGL"
     gene            complement(4929..8324)
                     /locus_tag="VEW08_04595"
     CDS             complement(4929..8324)
                     /locus_tag="VEW08_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYJ40045.1"
                     /translation="MGLVDFAIRRRVTIAMATVAITLLGVISLSRLKVNLLPDLSYPT
                     LTIRTELPGAAPTEVENLISRPIEEAAGVVRNVRSVRSVSRSGQSDVIIEFTWGTDMD
                     FAGIEVRERLDLLWLPTEATRPLLLRFDPSSEPVMRVALVDETAATSRNRAGSHEDRL
                     KFLRRFADDRIKPEIESVEGSAAVKVSGGFEDEVQIYVDQQRLAQLRLSIEQVAKRIG
                     AENVNLSGGRLEQGTQRFLVRTVNEFDTLDDMANAVIATVDGQPVYLKDVARVERGYK
                     DRTAITRLNGAECIELAIYKEGDANTVQLAQGIRGKLEALEKTLPSGTKLVPVYDQSK
                     FIASAVSDVKDAAILGGLLSILVLYFFLRDAWATIVTGIVIPVTVVGIFVMMYAFDLT
                     LNVMSLGGIALSVGMLIDNSVVILEAIARRKEAGLSTIDAAREGTAEVATAVTASTLT
                     SVAVFFPMVFVSGIAGQLFRDQALTVTFAQLISLMVGITLVPMLTAWRARLGDKEQEL
                     AADAGEPRETLVALRRYLQALGTNFRVQYIVRVPEDTRSFLRRIWRWFTWSLRFLVTA
                     VVRVLRIFFGLLGKLFAVLLSPFVWVTQKGYNVLDRHYPALLDWALRRRAAVLLTAFG
                     LLAATALILPRLGTELIPQLSQGEFTVKMRLPAGSPLETTDAQVQVIHSAAGKLPSLE
                     SAYSVAGTGNRLDANPVDSGENTGNLDVKLRAPIDKEGEERAIQELRTRLASIPGAQY
                     EFTRPSLLTLSTPVEVILAGYDLERLGLAASAVRARMEASGAFKDIRSSIEGGHPEIQ
                     ILFDQERASQLGLAVRDIADRVVSNVRGDVATRYRLQEKKIDVLVRSVDTRAASIEEV
                     RNLVVNPGSDRPVPLSAVAEVRLATGPAEIRRANQERVAVISAAPVRGDLGEATVTAQ
                     EILAQTTLPVGILGSVSGQSEEMTQSFRSLGLAFALAVFLVYLVMASQFESLLHPFVI
                     LFTIPMGLIGSVWGLYITGTTINSVALIGLIMLAGIVVNNAIVLTDAINQARERGLEK
                     IEAIKLAGKTRLRPILITSVSTIIGLIPMAIGIGEGAEIRRPMAITVIAGNLVATFLT
                     LIVIPVLYAVLDRKAYVKSAAGGTAARPLTTPAAAPASSPTEG"
     gene            complement(8327..9415)
                     /locus_tag="VEW08_04600"
     CDS             complement(8327..9415)
                     /locus_tag="VEW08_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ40046.1"
                     /translation="MQTALLRAAVIALALTVSACAKDGVAGDKTSQEKEAKALADSAI
                     PVEVAKPVRGEMLAMYSGTATLDAEADAEIIAKVGGEVRRILVEEGDHVESGQLLAVL
                     DDRQLRLQAAQTRAELAKAERDFNRQVELHQKGLVSAGAFEGLKYDLDNQRAAHDLAS
                     LSLSYSEIRAPFAGIVSARHVKLGQEIAIGTSVFRVTDPTPLKAAVYVPERELARLKI
                     GQAASISVDALAGRAFPAIVKLVSPTVDAATATFKVTLEVNDPKGDLKPGMFSRVGIV
                     FERRTEALTIPRIALLDTDGASNVFVVASGKAEQRAIKTGLSNAGKVEVTEGLTGTEQ
                     VVIVGQNGLKDGNPVRVVSLEQTAKAGS"
     gene            complement(9532..11655)
                     /locus_tag="VEW08_04605"
     CDS             complement(9532..11655)
                     /locus_tag="VEW08_04605"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HYJ40047.1"
                     /translation="MNSLITRSTRWLAPSMLAALLVLSGSAQAPAGTQLPAGSVLPSE
                     RHRSVARKVGQMLEGMHYSQSKLDDKLSAIVYARYLESIDSQHTSLLASDVVEFEQYK
                     LRFDDMIRSGDVDPAFAIFARFQQRNRERIKYAISQLDKEPNWTLNESFEFNREKSPW
                     AASSTELDDFWRKRVKNDALSLLLTGKNWEETSKLLKKRYETTLKRIDQVSSDDVFES
                     LMNSYARAYDPHSSYFSPRSSEEYRIQMSLNYDGIGASLQLIDDYVTIMNVIEGGPAA
                     VAGSLSVKDRITGVGQGKEGPVTDVLGWRIDDVVQLIRGRAGTQVRLQVLPAGAAPGT
                     PEKTLEFTRNKVTLEAQAAQKELRTIKRGDKQIRVGVINVPAFYQDVEAQNAGEKDFR
                     STTRDVRRLINELKAEKIDGLVLDLRGNGGGYLPEATALTGLFIDRGPVVQLKVTTGE
                     LEVQDDPEPGVAYAGPLAVLVDRFSASASEIFAGAIQDYHRGTVLGQRTFGKGTVQNL
                     VPLDRWSPRPVNGQLTVTIGKFYRVTGESTQHRGVEPDVTLPSQIDMEEFGESALEGA
                     LPWDRIPGVPFRSEGKAQQPNIAALATEEKTRADRDPDYRWLVADLAAADVMRKQTSL
                     SLNLQQRKDERERLDRERLTRENARRAAQNLPALKTAEELADAKFPDTVLDQAAEVMT
                     DMVTGARPLHSAPARTVRSESKPAG"
     gene            11756..12046
                     /locus_tag="VEW08_04610"
     CDS             11756..12046
                     /locus_tag="VEW08_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40048.1"
                     /translation="MRPLVVLLGIVMGSTVSIAAALLMTGVVFLLLPEYAERLAEERK
                     PLLTACLLAVLLAAAAATSFYGELRARSWRIAAHGALLVMLVLSLWTYWPKK"
     gene            12058..13716
                     /locus_tag="VEW08_04615"
     CDS             12058..13716
                     /locus_tag="VEW08_04615"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HYJ40049.1"
                     /translation="MNTFERLARHLVPTVLAGLAIGATAAPKSSPIDEGTYRAHIARL
                     ASDEFEGRKPGTEAEKRTLQYLESQFRELGLKPGNGASFLQEVPMVEITAAPDAALKF
                     TTGANTVDLKFRQDMVIWTKREKTAESMEASPMVFVGYGVTAPESGWDDYAGVDMRGK
                     TAIILVNDPGFATQDAALFRGRAMTYYGRWTYKFEEAARRGATGALIVHQTDAAGYGW
                     DTVVNSWSTPQLDNVTANGNAGRVAIQGWVTESAAQQLFAANGKSFADAVKRANTRGF
                     RAEPMQSTATASLRNTIRRTNSHNVAALLPGTKRPDEYLVYMAHWDHLGRMPGCSGDC
                     IFNGAVDNATGTAGILAIAKAFAGARSKPGRSIVFLAVTLEESGLLGSAYYVDHPLFP
                     LAQTVAAFNMDAMHFGGPTRDVTVVNIGASQLEKYLADAARAQGRVLTAEPTPETGTF
                     FRSDHFNFAKRGVPALYIKGGIDDREHGAAWRQKYLADFTAQKYHKVGDEYSPGADLR
                     AGVEDLSLLYAVGAKLAAEKSFPNWNQDSEFRSARDRSRAAAAK"
     gene            complement(13754..13978)
                     /locus_tag="VEW08_04620"
     CDS             complement(13754..13978)
                     /locus_tag="VEW08_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40050.1"
                     /translation="MSDYYLADLKAEERARQRQRELEPTRAPKKSQDMKVTLLGDFLF
                     PSSEPQGCDPYNSMNGKSAREAWRTRRDRR"
     gene            complement(14094..14450)
                     /locus_tag="VEW08_04625"
     CDS             complement(14094..14450)
                     /locus_tag="VEW08_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40051.1"
                     /translation="MRYYARAFPPAGGATGAWPKLFNWFVRAHAYYFSRPRLQFEAMT
                     LGLALLFGLLVMPALIYLPGYYVLKPYANGGLFALYVDYFKGLFEPRPSCWIALAGPF
                     VFLSLWRIFRLVLRKL"
     gene            14449..15408
                     /locus_tag="VEW08_04630"
     CDS             14449..15408
                     /locus_tag="VEW08_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYJ40052.1"
                     /translation="MSKIRAAVIGVGYLGRFHAQKYAALPDCELVAVVDGRDEVRKAV
                     AAEVATQPVADYRDLLGKVDAVSVVTPTPAHFEITEAFLAAGAHVLVEKPITETPGQA
                     RALIATAAKHGKILQVGHLERFNAAVLAAEPHISSPRFMECIRLAPYKERGTDVNVVL
                     DLMIHDIDLVQSLAASEIVSIDAIGTPVFSGEIDIANARIRFANGCVANVTASRVSMK
                     TERKLRIFEDAAYISLDLQQKILTLLRKRDGVPQPGQLPMSIDEENLDQGDALKSEIE
                     SFLDCIRNNKRPIVSGEDGLRALETAIRITEQVHANLARTPAG"
     gene            15454..16182
                     /locus_tag="VEW08_04635"
     CDS             15454..16182
                     /locus_tag="VEW08_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HYJ40053.1"
                     /translation="MNTPIRPLNDMRRTLDIDLVADFTCPWSWLGMAQLTRALGNLSG
                     DIETRLRWHPFRMASPNANTAPRSFHDYLAERLPAGISLPFAEKSLAEAGRELGIEFH
                     FEKLGALPSTSEAHRLVQLAANEGRHANVAAGIFRAFFESGADIGDVAVLEKIGAEAG
                     LDERTLKTFRETRDGESLLAGSEERLRGFGVRTVPNLLFGGRVLVPGTVNVATYVHAL
                     DQALFPSNDDPANEGEQKQKPTLH"
     gene            16347..17726
                     /locus_tag="VEW08_04640"
     CDS             16347..17726
                     /locus_tag="VEW08_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYJ40054.1"
                     /translation="MSQAGLKTDLRAIVGEQGMLEGAAVRERAGTLWHGRIDAELLVR
                     PRNTGQVSAVLKLCHARGQPVVTHGGLTGLVNGADADARDIILSLESMSAIERVDVPG
                     RTLRAQAGAKLGQVQRAAEENGMVFPLDLGARDSATVGGNISTNAGGLRVLRYGMMRN
                     LVLGIEAVLADGTVLTSLNRMLKNNAGYDLKQLFIGSEGTLGVVTRAELRLVSRTRSQ
                     ETMLASLPSFDALVELLGRLDSGLGGQLAAFEALWGNYYDYNTAPPAPNNAPLMRGAP
                     FYAIAETLGGDPEHDRARLESVLAQALEDGLLSDVTIASSETERRAIWAIREDVWQVK
                     NIAPLLTFDVSLPIENMKAYTEEVCGAVQAFAGPNRAFVFGHMADGNLHLVIAAGDDA
                     ATRATIEGMIYRPLAAIGGSVSAEHGIGLEKRAYLPLSRTAAEISTMRLLKNALDPKG
                     ILNPGKVFA"
     gene            17723..18643
                     /gene="aguB"
                     /locus_tag="VEW08_04645"
     CDS             17723..18643
                     /gene="aguB"
                     /locus_tag="VEW08_04645"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HYJ40055.1"
                     /translation="MSTPAHLKRPSREVVVAATQFACGWDVAANIANAEKLVRQAAKQ
                     GAQIILIQELFETPYFCIEQDSRHLRIATSVADNRAISHFAKVARELNVVLPISFFEK
                     ANNSYFNSIAIIDADGRNLGVYRKAHIPNGPGYQEKNYFSPGDTGFKVWDTKYARIGV
                     GICWDQWFPECARVMALMGAELLFYPTAIGSEPPPAMPVSSRDHWQRTQQGHAAANLM
                     PVIVSNRIGTERALQDPESVFIRFYGSSFIADSTGAKLAEADEVSEAVLTAKFDLAAN
                     EEMRNNWFVFRDRRPELYGALTTYDGGTRG"
     gene            18636..18857
                     /locus_tag="VEW08_04650"
     CDS             18636..18857
                     /locus_tag="VEW08_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HYJ40056.1"
                     /translation="MGNEKELAEIDALFAQADLDHDGQINFTEFKALVRELDGDMTEA
                     ELRVGFTETDTNQNSRINIDEFRAWWLSA"
     gene            complement(18892..19243)
                     /gene="ssrA"
                     /locus_tag="VEW08_04655"
     tmRNA           complement(18892..19243)
                     /gene="ssrA"
                     /locus_tag="VEW08_04655"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(19326..21353)
                     /locus_tag="VEW08_04660"
     CDS             complement(19326..21353)
                     /locus_tag="VEW08_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ40057.1"
                     /translation="MTTDYRTKILVPALLTLSFATAIHAAPPPVDAFGRKPAVIDVDV
                     NPAGTRLAWLEENGNISRLIIIDLTTGKDLRTVTAPDGVKLWRLAWANDESVLISKSE
                     TQKDPGMPKQKFEMQRWSAIDVAGGSDRVMLMQDGNRDLVSGASLLRARTATPGKIYM
                     STLDFSATNYKQETGSRLSGGRKDSGWIHNAYEVDVKNGKSKLIASGSSFTREYLVDH
                     SGTKIVRSDFNPQQREQSILVKAGISWRSLYRSKECGELDLRGFSADGAAVLALGSTC
                     DDGKNKFWSIPLDGTAMKSVFDDPALEIEGTVRDSVDGKVLAVSLGGQQQTVRWLDPD
                     AERRSKALHKSFGARWITFVSRSADNQKVVVLVEDETHPPIHYLVDYAAKKADILNEA
                     YPLLSGVKLGTVRDFNYEARDKYSLTGYLTLPSGAVEKGLPLVVMPHGGPEARDETGF
                     DWLAQFLASRGYAVLQPQFRGSTGRGRAHADAGRQQWGLRMQDDVTDGVKALITQGIV
                     DPKRVCIVGWSYGGYAALAGAAFTPDLYACAASIGGVSDLPVMLGAINRDDGRDSDSF
                     AYWREHIGSPFDPDVIAKSPARAAATVRAPILLLHGTNDTVVPIDQSRLMASALRKAS
                     KPVELIELTGEDHWMKTSSNSRIRTLTELERFLWQYIGSVQPAAAAASATN"
     gene            complement(21376..>21669)
                     /gene="smpB"
                     /locus_tag="VEW08_04665"
     CDS             complement(21376..>21669)
                     /gene="smpB"
                     /locus_tag="VEW08_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0070929 - trans-translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HYJ40058.1"
                     /translation="EAFLFGANITPLKTASTHVIPDPVRTRKLLLNKSELSRLVGSVE
                     RKGYTLVPLDLHWKEGRAKLEIGLAKGKKQHDKRATEKDRDWQRDKARVMRRG"
BASE COUNT         3972 a         6638 c         7038 g         4021 t
ORIGIN      
        1 cccgcggtac gcaatgaaat ccgccgctgg cggcaggaaa attccacgat gacgcaggcg
       61 gaacttgcgc gcctctgcgg cgtcacacgg cagacgatca tagcgctcga agcggaaaaa
      121 tattcgccgt cgctcgagct cgcattccgg ctcgcgcgcg cgctcggccg caacctcgaa
      181 gaggttttca ggttcgaaga aaaataaatc ccgacgcgcg aactgcttca cgttcaattg
      241 gcttgagttt tgcgtcttga atgatcgcgt tggtccgcta tattcggact caatgttaag
      301 tgacacgtta tccatcgttt cctggcgttc gcgcccgcgc agtttcgtcg cgctcatgtc
      361 gctgtacgag agcaactacc tgcggctcgc gcaactcgcc ggcaatctcg cggcattggc
      421 cgacatgcgc gtgtcgcgcg tgcctgaaga ctgtgatttg cgtctcacgg tctgtgagcg
      481 ttcgccgtat accacgagct tcgctctcac ctacctgttt gaccccgaag gcctgaccac
      541 ctacccggac atgcgcgtcc gcgtctatca cgacgcccgc cttgtggagg cccaggaatg
      601 ggcatcgcaa cacgagcacg aggccctgcg ccgcctgcgg ggcgtggcgg agcgtgaact
      661 ggatcagcgt tgggcgcgga atacgatgtt aaataagtgg ctggagtact gcatcgatcg
      721 tggccacggg tttcgcacgt aactgacgtc gaggcccccg cgcaggggtt tatgaaagcg
      781 ctgaagactt tcggttttct cggcggcacc aatgccgcgg aaccgccgcc cgaagacggg
      841 cgtgtcgtcg agctgttccg cagccgcgca gagttgcgca agcagcacga ggagctgggc
      901 gccgagatgc agcgcctgcg cgatcgcctc aagcagcagg aggcggccac cggccgcgtg
      961 caggaattgc tcgagtcgct ggaaaagcgg ctcgaagcgg ccgacaccgg cctatcttca
     1021 ctggttttct atcacctgcg cgccctgtgg ggcgacgggc gcgaaatcat cggcttgctc
     1081 gtgcaggacc tcgtcgccaa gcaggaagaa cgcgaacgca aggcgtgggc gctggaggga
     1141 aatcgaaaga cgttccagag tcgccaggcc gccgaacagc agttgcgcca ggcggagtcg
     1201 ctcatggtcg cggcgcgcca gcaactggtg gcgttcgatg cgcgactcgc caaactcgac
     1261 aagtggtggc atcgctggca gcggcccacg gtcgtcgcgc agcggccagc actgcaggcg
     1321 gcggtggaag cagcggaggc cagcctggtg ggagcgcgag cgcgctgcga acagttggag
     1381 aaggcgggcg ccggcgagtt tccgggcatt tccgtcgagg cgaagcgcgc gatcaatctc
     1441 gccgcgatcg cctgcgccga agtgctgtgc ctgcggctgg cgcgtacctc gctggtgtcg
     1501 tcggcgcggg ccgccatggc gcgtcgcgag tcgcttgaat actacggcga cctcgccgcc
     1561 tgcgtgtcca tcatgagtga catcgtcaag gcacgaaacc tgctcaagca gcgcgccggc
     1621 atcacccagg aagtgcagca gcgcagcgat cggttgcgtt cgacggcgac ctacctcggt
     1681 gaattcgaaa ccgtgccgat tccggaatcc tgtggtgtat ccgaaggcga cgtgctggcg
     1741 catggctcac agggcgtgag ttccgccaaa ctacccaacg tggtggccga agacacctgg
     1801 gacctcttca aagtcctgct tcgctgattc ctgccggtga gggcggcgcg tcgaccacgc
     1861 gcgtgcggcg catgaatcgc ctgacccgtt cggcgccatt gtcgatccac acgtagaacg
     1921 caggcaccac gaacagcgac atcacggcgc ccgccagcaa gcctcccatg atggaccgcg
     1981 ccatcggcgc ataagacgga ccaccaccac ccacggcgag cgatgcatcg cccgccgcga
     2041 gtggcagcag tccaagggag gctgtcagcg tggtcatgag aatgggtcgg agccggtcgc
     2101 ggcccgcctg cacgatggcg gtcatgcgtt ccatgccggt cgagcgcagt tcgttgatgt
     2161 gcgccaccag cacgatgccg atgttcacga ctacgcctat caatatctgc acgccgatga
     2221 aggccatgag cgtgatggtg gtgcgcgtca cgaacagcag ccagatcacg ccgatgatgg
     2281 ccatgaagat agaggtgatg atggacatcg gatacacggt gctttcgaat accgcggcca
     2341 tcaccaggta gatcattgcg atcgcgagca gcaggttgaa cagcatggtc tgcgaatttt
     2401 cgtcctgctg atcgaatccc ttgccgagct tccacgtgta tcccggcggc agctggtagt
     2461 tcttcatgag cgcttcgact tccttgccca cggcatcgag cgtcgattct ttcttcacat
     2521 tgccggtgat ggcgaccgag gtgaggcggt ccacacgttc gattgctcgc ggccccctgg
     2581 cgatgcggaa atccgcgacc gccgacagcg gcacgcgcgt gcccgtcggc aggtagatgg
     2641 gaaattccgc caggttgtcg acggtctggc ggtcggattc gcggaacgcc aggcgcagcg
     2701 tgagttcgcg ttcggtaccg cggaactcgc gcaagcggtc accgcgcatg ccggcggcga
     2761 cggcctgcgc gacgtcggaa gaggtgagcc cgagcgcggc cgcacgctgc cggttgacga
     2821 tgatctgcac ttcctcgtcg ccttcgcgcg cttcggaacg cacggcttcg aggcccttca
     2881 cgctcgacat gacgcgtgcc acttcatcgc cgagttccgc gagtttttcc gtggactcgc
     2941 ccgtgagctg cacgctgaag cgctgaccgc ccatggaatt ctcgtcatcc cacttgaagc
     3001 tcggtttgcc gatgatgatt tccggcagcc ctgcctgtgc tttctcgatg agttcctgtg
     3061 acttgggccc gccctcgtcg cgaggcttga gcacgaggat ggagaaagcg ctcgactggt
     3121 cgtagaccga gtagacactc tttatgccga gctcttcgcg gcgcttctca tcgaaatgac
     3181 gctcgatggt ttccaccgct gcttccacgc gatcgatcgg atgtgagccc ttgatgttgt
     3241 agccgaggaa cacctggccg ccgccatcct gcgcacccgg atcgaacttg atgaattttc
     3301 ccgggaagat attcgacagc acgatgaggc caacggtgag agcaagaacg gcaaacaatc
     3361 cgaacgccgt ccagccgcgg tgatgaatgc tccagtcgag cgtacgggta tagcgatcct
     3421 gcaggcgcga gatccagctc ctgctgtcca gcggcggagg tgcaggaaag cgcgaggtca
     3481 ccatggggat cagcgtctgt gcgatgacga gcgaagcgac catggccacg cagatcggaa
     3541 ttgccgcgtg agtaagccag atcgacagtt ggttgctcga gccgaacaga atgggcagga
     3601 acaccacgac gcaggttgcc gtgccggcga gggtggcgac ccccacctcc ttgacgcccg
     3661 ccagcgtcgc cgcctgcggg tgttcggggt ccatctgtcg atgacggaac acgctctcgg
     3721 tgaccaccac ggcgttatcc accagcatgc cgatggccag catcatgccc atcatcgaca
     3781 tcacgttgat ggtgagtccg acgaagtaca tgaccgcgag cgtgaccagc agtgacagcg
     3841 gcaccgagac gctgacgatg agcgtggtcg gccagtgccg caggaacagg aacaacaccg
     3901 tgaaggcgag ggcggcgcca atgagcccgg cctcggtgag atcgctcaac gaactgcgga
     3961 ttgcctcggc ctggttgtcg atggtgaata cgcggatgcc ctgcatctgc ggcagcgcct
     4021 tgttgtgttc gatgacttcg agcacacgat cgacgacgtc gaccacgttg gcctgcgtag
     4081 acttgaagac gtcgagcccc accgccggcc ggccatcgag gtgacgccgg cgcgtgatct
     4141 ccggactcac cagctccacg ctggcgacgt cgcccagatg cacgttgccc ttgatgacca
     4201 ggttgcggat ttcgtcgagc gaacggaatt cgccgagcgg ccgcaccgac aggcgctcgc
     4261 cgttgccggt gatctgtccg gccgccaccg agaagttgct cttctcgagc agcaggacga
     4321 gctggcggat gtccacggcg tgcgcggcca gccggcccgg atccaccagg atgcgcactt
     4381 cgcgcgcttc cacacccgcg agttcgacgc gcgccacacc atcgagacgc tcgatgggtt
     4441 tcttgaaata gcggtcgagc aggtcgtact gcgaagacag gtcctgatcg gcggaaaagc
     4501 gcaccgtcag catcggggaa tcggcggtgg aggccatcca catccacatg cgatccgcgg
     4561 tcttcggcaa gctcgaacgt accgaatcca gcttgtttcg aacctcgaat cccgcggtgt
     4621 tgatgtccgt gccccaggca aagcggattt caaagcgcgc gtcctggtca ctggacgtgg
     4681 agcggatttc ctcgatgccg ggcagcgtgg ccagcgcgtc ctcgatgggc cgcgtgatca
     4741 gttcctcggt ctcctcgggt gtcgagccgg gatagggcac gcccgcgccc atgaatggaa
     4801 agctgacgtc cggatactgc tctagcggca acagccgcgc cgacacgatg ccgatcaccg
     4861 caaccgcggc aaacgccatg agcgtggtga cgggccgtcg cagcgcgaag tcggtgtgga
     4921 ccatggcttc agccctcggt cggcgagctg gccggcgccg cagccggcgt agttagaggc
     4981 cgcgcggccg tcccaccggc ggcggacttc acgtatgcct tgcgatcgag gacggcgtac
     5041 aacaccggga tcacgatgag cgtgaggaac gtggccacca ggttgccggc aatcacggtg
     5101 atggccatgg ggcggcggat ctcggcgcct tcgccgatgc cgatggccat gggaatgagg
     5161 ccgatgatgg tgctgacgct ggtgatgagg atggggcgca gtcgcgtctt gccggcgagc
     5221 ttgatggctt cgatcttctc gagcccgcgt tcacgcgcct ggttgatggc gtcggtgagc
     5281 acgatggcgt tgttcaccac gatgcccgcc agcatgatga ggccgatcag tgccaccgag
     5341 ttgatggtgg tgccggtgat gtacaggccc cacaccgagc cgatgagccc catgggaatc
     5401 gtgaacagta tgacgaaggg atgcagcagc gattcgaatt gcgaggccat cacgagatag
     5461 accaggaaca ccgcgagcgc gaacgccagg cccagcgacc ggaaactctg ggtcatctct
     5521 tcgctctggc ccgacaccga tcccaggatg ccgacgggca gggtggtttg cgcgaggatc
     5581 tcctgtgccg tgaccgtcgc ctcgccaaga tcgccgcgaa cgggcgcggc agaaatcacc
     5641 gccactcgct cctggttggc gcggcggatt tcggcaggtc ccgtggccag gcgcacttcg
     5701 gcgaccgccg agagcggcac cgggcgatcg gatcccgggt tgaccacgag attgcgaact
     5761 tcctcgatcg acgcggcgcg cgtatcgacg ctgcgcacca gcacgtcgat tttcttttcc
     5821 tgcaggcgat aacgcgtcgc gacgtcgccg cgcacgttcg acaccacgcg atcggcgatg
     5881 tcgcgcaccg ccagcccgag ttgtgaggcg cgctcctggt cgaacaggat ctggatttcg
     5941 ggatggccgc cttcgatgct cgaacggatg tccttgaacg caccgctcgc ttccatgcgc
     6001 gcgcgtacgg cgctggcggc gaggccgagg cgctcgaggt cgtagccggc gaggatcacc
     6061 tcgaccgggg tggagagtgt cagaagtgaa ggccgcgtga attcatactg cgcgcccgga
     6121 atgctggcga ggcgcgtgcg cagttcctga atcgcgcgtt cctcaccttc cttgtcgatg
     6181 ggtgcacgca gcttcacgtc gagattgccg gtgttttcgc ccgaatcgac cgggttggcg
     6241 tccaggcgat tgccagtgcc ggctaccgaa taggcacttt cgagagaggg cagcttgcca
     6301 gccgcgctgt ggatgacctg cacctgcgcg tccgtcgtct cgaggggcga tcccgcgggc
     6361 aggcgcatct tcactgtgaa ctcgccctgg gagagctgcg gaatcagttc ggtgccgaga
     6421 cgcggcagga tgagtgcggt ggccgcgagg aggccgaacg ccgtgagcag cactgccgca
     6481 cggcgcctca gcgcccagtc gagcagcgcc gggtagtggc ggtcgagtac gttgtagccc
     6541 ttctgggtga cccagacgaa cggcgagagc aggacggcga acagcttgcc gagcaatccg
     6601 aagaagatgc gcaacacgcg gaccacggcg gtgacgagga agcgcagcga ccaggtgaac
     6661 cagcgccaga tgcgccgcag aaaactcctt gtgtcttcag gaacgcggac gatgtattgg
     6721 acacggaagt tagtgcccag cgcctggaga tagcggcgca gggcgacgag cgtttcacgt
     6781 ggctcaccgg catccgccgc cagttcctgc tccttgtcgc cgaggcgcgc gcgccacgcg
     6841 gtcagcatgg gcaccagcgt gatgcccacc atgagcgaga tgagctgggc aaacgtgacc
     6901 gtgagcgcct ggtcgcggaa caactgcccg gcgatgccgc tcacgaacac catggggaag
     6961 aacacggcga ccgacgtcag tgtcgaggcg gtcacggcgg tggccacttc cgccgtgcct
     7021 tcgcgcgcgg cgtcgatggt ggatagtccg gcttccttgc gcctggcgat ggcttcgagg
     7081 atgaccaccg aattgtcgat cagcatgccc accgagagcg ctatgccgcc cagcgacatc
     7141 acgttcagcg tcaggtcgaa ggcgtacatc atcacgaaga tgccgaccac ggtgaccggg
     7201 atcacgatgc cggtgacgat ggtggcccac gcgtcgcgca ggaagaaata cagcacgagg
     7261 atcgacagca ggccgccaag gatcgcggcg tccttcacgt cgctcacggc gctcgcgatg
     7321 aatttggact ggtcgtacac cggcacgagc ttcgtgccgc tgggaagcgt cttttcgagt
     7381 gcctcgagct tgccgcgaat gccctgggcg agttgaaccg tgttggcgtc gccttccttg
     7441 tagatggcga gttcgatgca ttcggcgccg ttcaggcgcg tgatcgcggt gcggtccttg
     7501 taaccgcgct ccacgcgcgc gacgtccttc agatagacag gctgtccgtc gacggtcgcg
     7561 atgaccgcgt ttgccatgtc gtcgagggtg tcgaattcgt tgaccgtgcg taccaggaaa
     7621 cgctgcgtgc cctgttccag ccggccgccg gagaggttga cgttttccgc gccgatgcgc
     7681 ttggccacct gttcgatcga gagccggagt tgtgcgagtc gttgctgatc gacatagatc
     7741 tgtacctcgt cttcgaagcc accgctgacc ttcacggccg cggaaccttc caccgattcg
     7801 atctccggct tgattcggtc atcggcaaag cgccgcagga atttgaggcg gtcttcgtga
     7861 ctacccgcac ggttcctgga agtggcggcg gtttcgtcca cgagcgcgac gcgcatgacc
     7921 ggttcgctgg acggatcgaa tcgcagcagc aaggggcggg tggcttcggt cggcagccac
     7981 agcaggtcga ggcgttcgcg cacctcgatg ccggcgaagt ccatgtccgt gccccaggtg
     8041 aactcgatga tcacgtcgga ctggccggat cgcgacaccg accgcacgga acgcacattg
     8101 cgcacgacgc cggccgcttc ctcgatgggg cgcgaaatca ggttttcgac ttccgtgggc
     8161 gcggcgcccg gcagctcggt gcgaattgtg agcgtcggat aggagaggtc cggcagcagg
     8221 ttcaccttga ggcgcgagag ggaaatcacc ccgagcaggg tgatcgccac cgtcgccatg
     8281 gcgatggtga cgcgccggcg aatcgcgaag tccacgagac ccacggctaa ctaccggctt
     8341 tggcggtctg ttcgagcgat acgacgcgta ccggattgcc gtccttgaga ccgttctggc
     8401 cgacgatcac cacctgctcg gtgccagtga gcccttcggt cacctcgacc tttcctgcat
     8461 tggagagccc ggtcttgatg gcgcgctgtt cggccttgcc actggcgacc acgaacacat
     8521 tagatgcacc gtcggtatcg agcagggcga tgcgcggaat cgtcagtgct tcggtgcggc
     8581 gttcgaatac gatgccgacg cgcgagaaca tgccaggctt gagatcaccc ttcgggtcgt
     8641 tgacctcgag cgtgaccttg aacgtggccg tggcggcgtc caccgtgggc gagaccagct
     8701 tcacgatggc ggggaaggcg cgaccggcga gggcatcgac gctgatcgat gccgcctggc
     8761 cgattttcaa acgggcaagt tcgcgttccg gaacgtacac cgcggccttg agcggcgtgg
     8821 gatcggtgac gcgaaacacg ctggtgccta tggcgatttc ctggcctaac ttcacgtgcc
     8881 gcgccgaaac gatgccggcg aacggcgcgc gaatctcgct gtacgacagg ctcagcgaag
     8941 ccaggtcgtg ggcggcccgc tgattgtcca ggtcatactt gaggccttcg aacgcgcccg
     9001 cggacaccag gcctttctgg tggagttcga cctggcgatt gaagtcgcgc tccgccttgg
     9061 cgagctccgc gcgggtttgg gcagcctgca gccgaagctg acggtcatcg agaaccgcga
     9121 gcagctggcc ggattcaacg tggtcgcctt cttcgacgag gatgcggcgg acttcgccgc
     9181 cgaccttcgc gatgatttcc gcgtccgcct cggcatcgag agtggcggtg ccgctgtaca
     9241 tcgcgagcat ctcgccgcga accggtttgg cgacttcgac ggggatggcg gagtcggcaa
     9301 gcgccttggc ttccttctcc tgggaggtct tgtcgccggc gacgccgtcc tttgcgcagg
     9361 ccgataccgt cagtgccagc gcgatgacgg cggcgcgtaa gagagctgtt tgcatggcct
     9421 tcccctttgc ctgcggttat gacggcgcag acgcccaatt ggttgcgaat tgggctgaag
     9481 tctagggaag tctggtgttg tagtcaacta caacaccaga ggtgcggagg cttagccggc
     9541 gggtttggac tcgcttcgga ccgttcgtgc gggtgcggaa tggagagggc gggcgcccgt
     9601 caccatgtca gtcatcactt cggcagcctg gtcgagcacc gtatcgggga acttggcgtc
     9661 ggccagctct tcggcggtct tgagcgccgg cagattctgc gcggctcggc gggcgttttc
     9721 ccgcgtgagc cgttcgcggt cgaggcgctc acgctcgtcc ttgcgctgct gcagattcag
     9781 cgacagcgag gtctgtttgc gcatgacatc ggcggccgcg agatcggcaa ccagccagcg
     9841 atagtccgga tcgcgatcgg cccgggtctt ttcctcggtg gcgagcgccg cgatgttcgg
     9901 ctgctgcgcc ttgccttcgc tgcggaacgg cacgccgggg atgcgatccc agggcagggc
     9961 gccttcgagt gcgctctcgc cgaattcttc catgtcgatc tgcgagggca acgtgacatc
    10021 gggctcgacg ccgcgatgtt gcgtgctttc gccggtgacg cggtagaact tgccgatggt
    10081 gaccgtgagc tggccattga ccgggcgcgg cgaccagcga tcgagcggca cgaggttttg
    10141 cacggtgccc ttgccgaaag tgcgctgacc gagaaccgta ccgcggtggt aatcctggat
    10201 ggcgcccgcg aaaatttccg aagccgacgc gctgaagcga tcgaccagca cggcgagcgg
    10261 gccggcatag gcgacgccgg gttccggatc gtcctgcact tcgagttcac ccgtggtgac
    10321 cttcagctgc accaccgggc ccctgtcgat gaacaggccg gtgagggcgg tggcttcggg
    10381 tagatagccg ccgccattgc cgcgcagatc gagcaccagt ccgtcgattt tctcggcctt
    10441 cagctcgttg atgagacggc gcacgtcgcg cgtggtgctg cggaaatcct tttcgccggc
    10501 gttctgcgcc tcgacgtcct ggtagaacgc cggaacattg atgacgccga cgcggatctg
    10561 cttgtcaccg cgcttgatgg tgcgcagttc cttctgcgcc gcctgcgctt cgagcgtgac
    10621 cttgttgcgc gtgaattcga gggttttctc cggagtgccg ggcgcggcgc ccgccggcag
    10681 gacctgcagg cgtacctgcg tgcccgcgcg gccgcggatg agctgcacga catcgtcgat
    10741 gcgccagccg agcacgtcgg tgaccgggcc ttccttgccc tggccgacac cggtaatgcg
    10801 gtctttgacg gacagggagc cggcgacagc ggcaggacca ccttcgatga cgttcatgat
    10861 ggtgacgtag tcgtcgatga gctgaagcga cgcgcctatg ccgtcgtaat tgaggctcat
    10921 ctggatgcga tattcctctg agctgcgcgg tgagaaatag ctggagtgtg gatcgtaggc
    10981 gcgcgcgtag gaattcatca gcgattcgaa tacgtcgtcg gagctcacct ggtcgatgcg
    11041 cttgagcgtg gtttcgtagc gcttcttcag cagtttggac gtttcttccc agttcttgcc
    11101 ggtgagcagc agcgacagcg cgtcattctt cacgcgcttg cgccagaagt cgtcgagttc
    11161 agtggagctt gcggcccagg gcgacttttc acggttgaat tcgaaggatt cgttcagcgt
    11221 ccagttcggc tccttgtcga gctgcgagat ggcgtatttg atgcgctcgc gattgcgctg
    11281 ctggaaacgc gcaaagatcg cgaaggcggg atcaacatcg ccgctgcgga tcatgtcgtc
    11341 gaatcgcagt ttgtattgct cgaattcgac gacgtccgac gcgagcaggc tcgtgtgctg
    11401 actgtcgatg ctttcgaggt agcgggcgta gacgatggcc gagagcttgt cgtcgagctt
    11461 cgactggctg tagtgcatgc cttcgagcat ctgcccgacc ttgcgcgcca cggaacgatg
    11521 tcgctcgctc ggcagcacgg agcccgcggg aagctgcgta ccggcgggag cttgcgcgga
    11581 gccgctgagt accaggaggg cggccagcat cgaaggtgcg agccagcggg tcgagcgggt
    11641 gatcaatgaa ttcatgcttc ggggtgtctc aagcttcggg acggtccgta caattcaaca
    11701 ctaaggccgc gcggcggcct gctcaagtgg catctcctca aacttaaggt cctacatgcg
    11761 cccattggtc gttctgcttg gaatcgtgat gggttcgaca gtctcgattg cggcggcgct
    11821 gctgatgacg ggggtcgttt ttctgctatt gccggaatac gcggagcgcc tggctgaaga
    11881 gcgaaaaccg ctgctgacgg cctgtctgct cgcagtgttg ctggcggccg ctgccgcgac
    11941 gagtttctac ggcgagttgc gtgcgcgctc ctggcgaatt gcggctcacg gagcgctgct
    12001 cgtgatgttg gtactttccc tgtggactta ctggccaaag aaatgaatcg cacccgcatg
    12061 aacacattcg agcgccttgc ccgacacttg gttccaactg tgctcgccgg actcgcaatt
    12121 ggcgctaccg cagccccgaa atcgagtccg atcgacgagg gcacctatcg cgcgcatatc
    12181 gccaggctcg catccgatga gttcgagggg cgcaaaccgg gcactgaagc ggaaaaacgc
    12241 acgctccagt acctcgaatc ccagtttcgc gagttagggc tgaaacccgg caacggggcg
    12301 agcttcctgc aggaagtgcc catggtcgag atcaccgcgg ccccggatgc cgcgctgaag
    12361 ttcaccacgg gcgcgaacac cgtggatctc aaattccgcc aggacatggt catctggacc
    12421 aaacgcgaga agaccgccga gtcgatggaa gccagtccca tggtgttcgt cgggtacggg
    12481 gtcactgcgc ccgaatctgg ctgggacgat tacgccggcg tcgacatgcg cggcaagacg
    12541 gccatcatcc tcgtcaatga cccgggattc gcgacgcagg acgctgcctt gttccgcggt
    12601 cgggctatga cctactacgg acgctggact tacaagttcg aggaggctgc gcgccgcggc
    12661 gccactggcg ccctgatcgt ccatcagact gatgctgccg gttacggctg ggacaccgtc
    12721 gtgaacagct ggtccactcc gcagctcgac aacgtcaccg ccaatggcaa tgccggtcgg
    12781 gttgccatcc agggatgggt cacggaatct gccgcgcaac aattgttcgc ggcgaacggg
    12841 aagtcatttg ccgacgcggt gaagcgcgcc aacactcgcg gcttcagagc cgagccgatg
    12901 cagagcacgg cgaccgcatc attgcgtaac acgatccgcc gtacgaactc gcacaacgtg
    12961 gcggcgctgt tgcccggaac gaagcggcct gacgaatacc tggtttacat ggcgcattgg
    13021 gatcacctgg ggcgtatgcc gggatgttcc ggcgactgca tcttcaacgg ggcagtggac
    13081 aacgccaccg ggaccgcagg gattcttgcc attgccaagg cattcgccgg cgcgaggtcg
    13141 aaaccggggc gctcaatcgt attcctcgcg gtcacactcg aagagtccgg tctcctgggt
    13201 tcggcttatt acgtcgatca tccgctgttt ccactggcgc agacggtagc cgcgttcaac
    13261 atggacgcca tgcacttcgg tggtcccacg cgggacgtta cggtagtcaa catcggcgca
    13321 tcgcagctgg agaaatacct ggccgatgcc gccagggcgc agggcagggt actcacggcc
    13381 gagccaacac cggagacggg cactttcttt cgctccgacc atttcaattt cgcgaagcgc
    13441 ggtgtgccgg cgctgtacat caagggcggt atcgacgatc gcgagcatgg cgctgcgtgg
    13501 cgccagaagt acctcgcgga tttcacggcg cagaaatacc acaaggtggg cgacgaatat
    13561 tcaccgggcg ccgacctgcg cgccggcgtc gaagatctct cgctgctgta cgcggtgggc
    13621 gcgaaattgg cggcggagaa gtcgtttccg aactggaatc aggacagcga gtttcgctcg
    13681 gctcgcgatc gcagtcgtgc cgcggccgcg aagtagccag aggggatgcc cgttcggccc
    13741 tgtgcgtgcg gactcagcgg cgatcgcgac gcgtacgcca ggcttcgcgc gcggactttc
    13801 cgttcatgct gttgtaggga tcgcaccctt gaggctcgga ggacggaaac aggaagtcac
    13861 ctaacaaggt gaccttcatg tcctggctct ttttcggtgc ccgcgtcggc tcgagctcac
    13921 gctggcgttg ccgcgcacgc tcctcggcct tcagatccgc gaggtagtag tcgctcatgg
    13981 gtttcaacct ccgcggtcag tgtctccgaa ttgcgtcaca attccggagg ttgacccctg
    14041 agggttctgg agaattcgtg aacgagttca cgttttccag cgcgtcgttt tacttatagc
    14101 ttgcgaagaa cgaggcggaa gatgcgccac aggctcagga agacgaacgg gcccgccagc
    14161 gcgatccagc agctcggtcg gggctcgaac aggcctttga agtagtcgac gtaaagtgcg
    14221 aacaggccgc cattggcgta cggcttgagt acgtagtagc cgggcagata aatgagcgcc
    14281 ggcatcacga gcagcccgaa aagcagggcg agccccagcg tcatcgcttc gaattggagt
    14341 cgcggtcgcg agaagtaata agcgtgcgcg cgcacgaacc agttgaacaa tttcggccag
    14401 gctccagtag ccccgccggc cggcgggaaa gcgcgggcat aatagcgcat gagcaaaatc
    14461 agggcggcag tcatcggtgt cggctatctc ggccgctttc atgcgcagaa gtacgcggcg
    14521 cttccggact gcgagctggt tgcggtggtc gacgggcgcg acgaggtccg caaggccgtg
    14581 gccgccgagg tggccacgca gccggtggcc gactatcgcg atctgctcgg caaggtggat
    14641 gcggtcagcg tagtcacgcc gacgccggcc cacttcgaaa tcacggaggc ctttctcgcc
    14701 gcgggcgccc acgtgctcgt ggaaaagccg atcaccgaaa ctcccgggca ggccagagcg
    14761 ctgattgcga cggcggcgaa gcacggcaag atcctgcagg tcgggcatct cgagcgtttc
    14821 aatgccgcgg tcctggcagc cgagcctcac atcagctcgc cgcgtttcat ggagtgcata
    14881 cgcctggcgc cctacaagga gcgtggcacg gacgtgaacg tggtgctcga tctcatgatc
    14941 cacgacatcg atctggtgca gtcgctggcc gccagcgaga tcgtgagcat cgacgccatc
    15001 ggtacgccgg tgttctccgg cgaaatcgac atcgccaacg cgcgcattcg cttcgccaac
    15061 ggttgcgtgg ccaatgtcac tgccagccgc gtcagcatga agaccgaacg caagctgcgg
    15121 atctttgaag atgccgcgta catttcgctc gacctgcagc agaaaattct gacactgctc
    15181 cgcaagcgcg acggagtgcc acagcccgga cagttgccga tgagcatcga cgaggaaaat
    15241 ctcgaccagg gcgacgccct caagagcgaa atcgagtctt tcctcgactg catccggaac
    15301 aacaagcgtc cgatcgtgtc aggagaagat gggctgcgcg cactcgagac cgccatccga
    15361 atcaccgagc aggttcacgc gaatctcgcc agaacccccg cgggttgacc catggcggct
    15421 tgaaggtgcc cgcggcgcac ctacatatgc cgcatgaaca caccaatccg acccttgaat
    15481 gacatgcgac ggacgctgga catcgatctc gtcgctgatt tcacctgccc gtggtcgtgg
    15541 ctgggcatgg cgcaattgac gcgggcgctc ggcaacctgt cgggtgacat cgaaacccgc
    15601 ctgcgctggc atccgttccg aatggcatcg ccaaatgcga atacggctcc gcgcagcttt
    15661 catgattacc tggccgagcg gctgcccgct ggcatcagcc tgccgtttgc ggagaagagc
    15721 ctggcggagg cagggcgcga actcggaatc gagtttcact tcgagaagct cggcgcattg
    15781 ccgagcacct ccgaggccca tcgcctggtc cagctcgccg caaacgaagg tcggcatgcg
    15841 aatgtcgccg ctggcatctt ccgtgccttt ttcgaaagcg gcgctgacat tggcgatgtc
    15901 gccgtactcg aaaaaatcgg cgccgaggcg ggactcgatg agagaactct caagacgttt
    15961 cgcgagacgc gcgatggcga atccctgctc gcgggcagcg aagagcgcct gcgaggattt
    16021 ggcgtgcgca cggtccccaa cctgctgttc ggcggacggg tgctggtgcc aggcaccgtg
    16081 aacgtcgcca cctacgtgca cgcgctggac caggcgctgt ttccatcgaa cgacgacccg
    16141 gcgaacgaag gcgagcagaa gcagaagccg acgttgcatt gagccaggtt gaatcgagcc
    16201 acgttgaatc gagccacgtt gaatcgagaa tgggcgcagg cagcgtcgtc ctggcggtag
    16261 tggcagcggc tgccgttatg gcggtacttg tctgatgagc agccgttatg gcggctgcat
    16321 gcaatgcgcg atacctttac ggccgcatgt cacaggccgg actcaagact gatctacgcg
    16381 ccatcgttgg cgagcaggga atgctcgagg gcgccgccgt gcgcgaacgt gccggcacgc
    16441 tctggcacgg acgcatcgat gccgaactac tggtgcggcc gcgaaacacc gggcaggtgt
    16501 cggccgtact caagctttgc cacgcgcgcg gtcaaccggt ggtcacccat ggcgggctga
    16561 cgggcctggt caacggtgcc gatgccgatg cgcgcgacat catcctgtcc ctggaatcga
    16621 tgagtgccat cgagcgagtc gatgttcctg gacgtacgct gcgagcccag gccggtgcga
    16681 agctcggtca agtgcaacgt gcggcggaag agaacggcat ggtctttccg ctcgatctcg
    16741 gtgctcgtga cagtgcgacg gtcggcggaa acatctctac caacgccggc ggactgcgcg
    16801 tcctgcgtta cggaatgatg cgcaacctgg tgctcggcat cgaagccgtc ctggccgatg
    16861 gcacggtgct cacgtcgctc aatcggatgc tcaagaacaa tgccggatac gacctcaagc
    16921 agctgttcat cggcagcgaa ggcaccctgg gggtcgtcac gcgcgcggag ttgcggctgg
    16981 tttcccgcac gcgcagccag gagacgatgc tcgcctcact gcccagtttc gacgccctgg
    17041 tcgaattgtt gggccggctg gattcgggtc tcggcgggca gctcgcagct tttgaagcgc
    17101 tgtggggcaa ctactacgac tacaacaccg cgccacccgc gccgaacaac gctccactca
    17161 tgcgcggcgc gccgttttac gccatcgcgg agacgctcgg cggagacccc gagcacgatc
    17221 gggcgcgcct cgaaagcgtt cttgcgcagg cactcgaaga cggtctgctc tcggacgtca
    17281 ccatcgccag ctccgagacc gagcggcgcg ccatctgggc gattcgcgaa gacgtgtggc
    17341 aggtcaagaa catcgcgccg ctgctcacgt tcgacgtgag cctgcccatc gagaacatga
    17401 aggcgtacac ggaagaagtg tgtggcgccg tgcaggcatt cgcgggtccg aaccgcgcat
    17461 tcgtgttcgg ccacatggcc gatggcaacc tgcacctggt gatcgccgcc ggtgatgacg
    17521 cagcgacgcg cgccaccatc gaaggcatga tttaccggcc gctggccgcc atcggtgggt
    17581 cggtttccgc cgagcacggt atcggcctcg aaaagcgcgc ctatctaccc ttgtcgcgca
    17641 ccgccgcgga gatctccacc atgcgattgc tcaagaacgc gctcgatccc aaaggcattc
    17701 tcaacccggg caaggtattc gcatgagcac tccagcgcat ctgaagcgtc ccagccgtga
    17761 agtggtcgtg gccgcaaccc agttcgcgtg tggctgggat gtggcggcga acattgccaa
    17821 cgccgaaaaa ctcgtgcgtc aggccgcgaa gcagggcgcg cagatcatcc tgatccagga
    17881 gctgttcgaa acaccctact tctgcatcga gcaggattcg cgccacttgc gcatcgccac
    17941 cagcgttgcc gacaaccgtg ccatcagtca tttcgcgaag gtggcgcgcg agttgaacgt
    18001 ggtgctgccc atcagcttct tcgaaaaggc gaacaattcg tacttcaatt cgattgccat
    18061 catcgatgcc gatggacgaa acctcggcgt ctatcgcaag gcgcacattc cgaacggccc
    18121 cggataccag gaaaagaatt acttcagccc gggcgacacc ggcttcaagg tctgggatac
    18181 gaaatacgcg cgcatcggtg tgggcatctg ctgggatcaa tggtttcccg agtgcgcgcg
    18241 cgtcatggcg ctcatgggcg cggaactgtt gttttacccg actgcgatcg gaagtgagcc
    18301 tcctcccgcg atgccagtca gttcgcgcga ccactggcag cgcacccagc aggggcacgc
    18361 ggcggcaaac ctgatgccgg tgatcgtctc gaatcgcatc ggcaccgaga gggcgttgca
    18421 agatcccgaa agcgtcttca tccgcttcta cggttcgagt ttcatcgccg actccaccgg
    18481 cgcgaagctt gcggaagccg acgaagtgag cgaggcggtg ctgaccgcaa agttcgacct
    18541 ggccgcgaac gaagagatgc gcaacaactg gttcgtattc cgcgatcgcc ggcccgagct
    18601 gtacggtgcg ttgaccacat acgacggagg gacccgtggg taacgaaaag gagttggccg
    18661 aaatcgatgc gttgttcgcg caagcggacc tggatcatga tgggcagatc aatttcaccg
    18721 agttcaaggc gctggtgcgc gaactcgacg gagatatgac ggaagcggag ctgcgcgtcg
    18781 gattcacgga aaccgatacg aatcagaaca gccgcatcaa catcgatgaa tttcgcgcct
    18841 ggtggttgag cgcctgagcg cacccgtcac ggccgggacc gtgacgggaa ttggtggagg
    18901 cggcgggaat tgaacccgcg tccgcaagcg ttagacgtaa gactctacgt acgtagctgc
    18961 ttcattattt tctcgcttcg cgctacccga agagcaggga aaacgctcgg ccagcgactg
    19021 tgactcttaa cgattccgcc agtagcacac ggaaacgcga tcctatgaac gctaccccag
    19081 gtccagcggc ataggcacca actggtctga gggcaaccgc gattaggcgg ctagtgcgta
    19141 gttgtcgtcg ttggcaacta tgaagtgcac agtgtttaac gagggccatg cgcctcggta
    19201 cgcctcttac ggttcactac ccacgtcgaa accggtcgcc cccgtaaagg tgaaggcagc
    19261 atacatgggg gaaccgggcg cgtctgcaag gcaggcagca ttgctcaaca aatgggcgtt
    19321 attcgtcagt ttgtggcgga ggcggcagct gcggccggct gcacgctgcc gatgtactgc
    19381 cagaggaatc gctcgagttc ggtcagcgtg cgaattcgcg agtttgagct cgtcttcatc
    19441 cagtggtctt cgcccgtcaa ttcgatgagc tctaccggct tgctcgcctt gcgtagcgcc
    19501 gacgccatga ggcgggattg atcgatgggg accacggtgt cattcgttcc atgaagcagc
    19561 agtatcggtg cgcgcacggt cgcggcagcg cgcgccggcg atttcgcgat gacgtccgga
    19621 tcgaatggcg atccgatgtg ttcgcgccag tatgcaaacg agtccgaatc gcggccatcg
    19681 tcgcggttaa tggcgccgag catgactggc aagtcggaca cgccgccgat gcttgccgcg
    19741 caggcgtaca ggtcgggagt gaatgcggca ccggcaagcg ccgcgtagcc gccataactc
    19801 cagccgacga tgcaaacgcg cttggggtcg acgatgccct gcgtgatcag cgccttgacc
    19861 ccgtcggtga catcgtcctg catgcgcagg ccccactgtt gccggccggc atcggcatga
    19921 gcgcgcccac gcccggttga gccgcggaac tgtggttgca gcacggcata gccgcgcgat
    19981 gccaggaatt gcgccagcca gtcgaagccg gtctcgtcgc gggcttcggg gccaccgtgc
    20041 ggcatcacca ccagcggcag acccttttcc acggcgccgg agggcaaggt gagatagccg
    20101 gtcagtgagt acttatcgcg cgcctcgtag ttgaagtcgc gtacggtgcc gagtttcaca
    20161 ccactgagta gtggataggc ctcattgagg atgtcggctt tcttcgcggc gtagtcgacc
    20221 aggtagtgga tcgggggatg agtttcgtct tcgacgagta ccacaacctt ctggttgtcc
    20281 gcggaccgac tcacaaacgt gatccagcgc gcgccaaaac tcttgtgcag cgccttggag
    20341 cgccgctcgg catcgggatc taaccaccgg accgtctgtt gttgcccgcc gagtgacacg
    20401 gcgagcacct tgccgtccac gctgtcgcgt accgtgcctt ctatctccag cgccggatcg
    20461 tcgaacacgg atttcatcgc agtgccatcg agcgggatgg accagaactt gttcttgccg
    20521 tcatcgcaag tggagcctag cgcgagcacg gcggcgccat ccgcgctgaa ccctctgagg
    20581 tcgagctcgc cgcattcctt ggagcgatac agactgcgcc agctaatgcc cgctttcacg
    20641 aggatggact gctcgcgctg ctggggattg aagtcgctgc gcactatctt cgtacccgaa
    20701 tggtccacga ggtattcacg cgtgaacgac gaccccgagg cgattaattt tgacttgccg
    20761 ttcttgacgt caacttcgta ggcgttgtga atccagccgg agtccttgcg cccgcccgac
    20821 agccggctgc cggtttcctg tttgtagttc gtcgctgaaa aatcgagcgt cgacatgtaa
    20881 atctttcccg gcgtcgccgt gcgcgcgcgc agcaggctcg cgccggagac gagatcccga
    20941 ttgccgtcct gcatcaacat cacgcgatcg cttccgccgg ccacgtcgat cgcagaccag
    21001 cgctgcattt cgaatttctg cttcggcatt cccggatctt tttgcgtctc gctcttgctg
    21061 atgagcacgc tttcatcgtt tgcccaggcc aggcgccaga gtttcacgcc gtcaggagcc
    21121 gtgacagtgc gcaggtcttt gccggtagtc aggtcgatga ttatgaggcg cgagatgttg
    21181 ccgttttcct ccagccacgc gagacgcgtg ccggccggat tgacgtcgac gtcgatgact
    21241 gccggctttc gtccgaaggc gtcgacagga gggggtgctg cgtggatcgc ggtagcgaac
    21301 gacagcgtga gcagtgcggg caccaggatc ttcgtccggt agtcagtcgt cacaatcaac
    21361 cctcccaggt ttggatcaac cgcgtcgcat gactcgcgcc ttgtcgcgct gccagtcgcg
    21421 atctttttcg gtggcgcgtt tgtcgtgctg tttcttgccc ttggcgaggc cgatctcgag
    21481 tttggcccgg ccttccttcc agtgcagatc gagcggtacc agcgtgtacc ccttgcgttc
    21541 gacgctgccg acgaggcgcg acagctcgga cttgttcaat aacaacttgc gcgtgcggac
    21601 cgggtccgga atcacgtgcg tcgacgcggt cttcagcggc gtgatgttgg cgccaaacag
    21661 aaaagcttc
//
LOCUS       DASQJS010000116         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3208088, whole genome shotgun sequence.
ACCESSION   DASQJS010000116 DASQJS010000000
VERSION     DASQJS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3208088"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VEW08_04670"
     CDS             <1..665
                     /locus_tag="VEW08_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HYJ40059.1"
                     /translation="KPAAIKIRFDNDRVDLDSEIPDHLAKARAVRKLVGNDFPLAFDA
                     NNGYTPGGAMRVGRVLEELGYWWFEEPVQHYHVRATGEVARKLSITVSAGEQSYTPAA
                     VADLILAGVTMVQPDIVKMGGITGMMRCAALAQAHGAEFVPHQTQPTIGHMASLHIAA
                     TQLHATKPCELNDPSPRMHAVFSNPPRPVDGVFHLPTAPGLGLDIDEAALAQRRVELP
                     GS"
     gene            complement(691..>2209)
                     /locus_tag="VEW08_04675"
     CDS             complement(691..>2209)
                     /locus_tag="VEW08_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HYJ40060.1"
                     /translation="PLTSKWLVMGAGMAGAVGMVLSSTLANVAVPTVMGAFGVGQDQA
                     QWLATGFVATMVASQLLSAWFARAFGVRGTFVLINCIFFVGTALSVVGTSFEMVVLGR
                     VLQGFAAGVMQPMIMALIFTQFPPHRRGQAMAIHGTGIQIAPMLGPMIGGLVIDTLGW
                     REIFLVPIPICVVALLLGMVYLPGREEQSRPGPRFDWTGYSLLVAMLATLLFAGANGQ
                     RFGWSSDAIVILVMIGLTAGGAFVWQQLRSTSPLLDFSLFRIPQFTSAVIVEFVFGFG
                     NFASNYLVPVTVQQVQGLTPFLSGLMLVPAGVLVILATPLFGRVADVFPAHLMVMLGV
                     SLFALGNYLMSQTDANTTFLAFAWLVIVARCGMALILPSLNTSALRALAPEQLHRGSG
                     TINFIRQLGGSCGVTVLVVFMEQRTQFHVEAMAATQTAANTTTLDLLSRVNALLAQAG
                     VSENLRGPGALHYLGEMIEEQAIARGFSDGFLIIALVFVLAVIPAWYLGKARRRP"
BASE COUNT          445 a          728 c          722 g          314 t
ORIGIN      
        1 acaagccggc ggcgatcaag atccgcttcg acaacgaccg cgtggatctc gacagcgaga
       61 tcccggatca cctcgccaag gcgcgcgccg tgcgcaagct cgtcggcaac gattttccgc
      121 tcgcattcga cgcgaataac ggctacacgc cgggcggcgc catgcgcgtg gggcgcgtac
      181 tcgaggagct gggatactgg tggttcgagg aaccggtaca gcactaccac gtgcgcgcga
      241 cgggcgaggt cgcgcgcaag ctgtcgatca ccgtctccgc cggcgagcag agctacaccc
      301 cggccgcggt ggctgacctt atcctcgccg gtgtgactat ggtgcagccg gacatcgtca
      361 agatgggcgg catcaccggc atgatgcgct gcgccgcgct ggcccaggcg cacggcgcgg
      421 agttcgtgcc gcaccagacg cagccgacga tcggccacat ggcgagcctc cacatcgccg
      481 ccacgcagct gcatgcgacc aagccctgcg agttgaacga cccgtcgccg cgcatgcacg
      541 ccgtcttctc gaatccgccg cggccggtgg acggcgtctt ccatctgccc accgcgcccg
      601 gccttggact cgacatcgac gaagcggcgc tggcgcagcg ccgggtggag ctgccgggaa
      661 gctgaacgcg gatgccatgg ttggaaattt ctacggccga cggcgcgcct tgcccagata
      721 ccacgccggt atgaccgcca gcacgaacac gagcgcgatg atcagaaagc cgtcgctgaa
      781 accgcgtgca atcgcctgtt cctcgatcat ctcgcccagg tagtgcagcg ccccggggcc
      841 gcgcaagttt tcggacaccc ctgcctgtgc cagcaatgcg ttcaccctgg agagcagatc
      901 gagagtcgtc gtgttggccg cggtctgggt tgccgccatc gcctcgacat ggaactgcgt
      961 gcgctgctcc atgaacacca ccagcacggt gacgccgcac gatccaccca attggcggat
     1021 gaaattgatc gtgccggatc ccctgtgcag ttgctcgggc gcgagcgcgc gcagtgccga
     1081 ggtgttgagc gaaggaagga tcaaggccat tccgcagcgc gcgacgatga cgagccaggc
     1141 gaacgcgagg aacgtcgtat tcgcatcggt ctggctcatg aggtagttgc ccagcgcgaa
     1201 gaggctcacg ccgagcatca ccatcaggtg ggcaggaaaa acgtcggcaa cacggccgaa
     1261 cagcggcgtg gccagaatga cgagcacgcc ggccggaacc agcatcaggc cggacaggaa
     1321 gggcgtcagg ccctgcacct gctggacggt gaccgggacc aggtaattgg acgcaaagtt
     1381 gccgaagccg aacacgaact caacgatgac cgccgaggtg aactgcggga tcctgaagag
     1441 cgagaagtcg agcagcggtg acgtcgatcg cagttgctgc cagacgaaag cgccgccggc
     1501 ggtaaggccg atcatcacca atatcacgat cgcatcggag ctccagccaa aacgctgccc
     1561 gttcgcaccg gcgaagagca gggtcgcgag catcgcgacc agcagcgagt agccggtcca
     1621 gtcgaacctg ggcccggggc ggctctgctc ctcgcgcccg ggcaggtaga ccatgccgag
     1681 gagcagcgcg accacgcaga tcgggatcgg cacgagaaag atttcccgcc agccgagggt
     1741 gtcgatcacc aggcccccga tcatcggtcc gagcatgggg gcgatctgaa tgcccgtgcc
     1801 gtggatcgcc atggcttgac cgcgacgatg cggcgggaac tgggtgaaga tgagcgccat
     1861 gatcatcggc tgcatgacgc cggctgcgaa gccttgcagc acgcgtccca gcacgaccat
     1921 ctcgaagctt gtgccgacga cagagagcgc ggtgccaacg aagaaaatgc agttgatcag
     1981 cacgaatgtg ccacgcacgc cgaaggcgcg cgcaaaccag gcgctcagga gctggcttgc
     2041 gaccatggtc gcgacgaacc ctgttgccag ccactgcgcc tgatcctgcc ccactccgaa
     2101 ggcgcccatc acggtcggca cggcgacatt ggcgagcgtc gacgagagca ccatcccgac
     2161 cgcgccggcc ataccggcgc ccatcacgag ccacttgctg gttagaggt
//
LOCUS       DASQJS010000117         7377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3832594, whole genome shotgun sequence.
ACCESSION   DASQJS010000117 DASQJS010000000
VERSION     DASQJS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3832594"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VEW08_04680"
     CDS             complement(<1..678)
                     /locus_tag="VEW08_04680"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HYJ40061.1"
                     /translation="MSHPRRKSVAVRVGRITVGGPAPIVIQSMTNTDTADIEGTARQI
                     KDLARAGSELVRITVNEASAAAAVAPIRDRLAQMGVDVPIIGDFHFNGHKLLREHPAC
                     AEALAKYRINPGNVGRGSKRDPQFAEMIEFACKYGKPVRIGVNWGSLDQDLLTRMMDE
                     NNSRAAPLSAQEIMREAVVASALESAARAVELGLPRERIILSAKVSGVQDLIAIYRRL
                     GERCDYPL"
     gene            complement(675..1124)
                     /locus_tag="VEW08_04685"
     CDS             complement(675..1124)
                     /locus_tag="VEW08_04685"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYJ40062.1"
                     /translation="MSHPRFLLLLLASLPLIALASPRGDEDSARAALQARFDAALHAD
                     VAALDKLLADDLEYCGVSATCSTKAQYLESVRSGTRKFRSLVPKVERVTLFADSAVLT
                     GKVYAVSIGDGTERNVDAYFLVVLAWRDDRWQMTNQSSTLLQPKPKT"
     gene            complement(1129..1545)
                     /locus_tag="VEW08_04690"
     CDS             complement(1129..1545)
                     /locus_tag="VEW08_04690"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYJ40063.1"
                     /translation="MMSIASFAKDGVREAALKAAEITRFEAQVNADAKVLGNLLDDGL
                     EYTHSNGDLDTKASFIDSLVTGKRDYISSVATVDSVRIFGDVGVIRGKARVTVSGKDR
                     QPQDLHIGYTDIWLWKDGRWQMTAWRSMRMPDSPVK"
     regulatory      1658..1750
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1939..3024
                     /gene="gcvT"
                     /locus_tag="VEW08_04695"
     CDS             1939..3024
                     /gene="gcvT"
                     /locus_tag="VEW08_04695"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HYJ40064.1"
                     /translation="MGRRTPLYDLHVGQGARMVDFGGWDMPVNYGSQIEEHHAVRRDA
                     GIFDVSHMCVIDARGPRVREFFSKLIANDVAKLTQSGKALYSCMLNEAGGVIDDLIVY
                     FLTESWFRVVVNAGTRDKDLAWMRGIAPQFGVELTERTDLAMLAVQGPNARAKVAELL
                     PAEHREAALALEVFFGRELGPWFVARTGYTGEDGFEIMLPADEAARVWRTLNERGVKS
                     AGLGARDTLRLEAAMNLYGNDMDENQNPLESGLAWTVSFENPARDFVGRRALESLKSA
                     GLEKKFVALLLEDRGVLRGHQKVVTGSGDGEITSGTFSPTMEKSIALARVPRGTGAAV
                     QVDVRGKLLNARVVKAPFVRHGKILVS"
     gene            3047..3433
                     /gene="gcvH"
                     /locus_tag="VEW08_04700"
     CDS             3047..3433
                     /gene="gcvH"
                     /locus_tag="VEW08_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HYJ40065.1"
                     /translation="MSNIPADLKYTKSHEWVRTLANGNVEVGITDHAQGALGDLVFVE
                     VPEAGKALKAGDSFAVVESVKAASDVYAPIAGAVVEGNAGLSSQPELINSEPYGAGWI
                     MRLKPGGAPSGLLSAAEYQAHLDAEG"
     gene            3437..4807
                     /gene="gcvPA"
                     /locus_tag="VEW08_04705"
     CDS             3437..4807
                     /gene="gcvPA"
                     /locus_tag="VEW08_04705"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HYJ40066.1"
                     /translation="MPFNPHTPKDVSDMLAAIGAKKIEDLFDEIPKELRIESLAGIPD
                     ALNEMHTGKLMMARAAQDGTPLNFIGAGAYEHHIPAAVWAVTTRGEFYSAYTPYQAEA
                     SQGTLQLIYEYQTMISSLTGMEVSNASLYDGGSAVAEASLMAIRAHRKSRSQRILVPT
                     TVHPNYRKVAVATATGQGVRFEEIACPKGQLSVADLKKYEGEDITAIVIQQPNFFGVL
                     EDVDAITDWAHANGIMVIGSVNPTSLAILKPPGEWGAKGADIACGDCQPLGVPLSSGG
                     PYAGFLTTRMEYVRQMPGRIVGRTVDASGHQGFALTLQAREQHIRRGKATSNICTNQG
                     LLVTAATIYLSLLGPEGLARVANESHRRTNELVAALTRIKGVKLAFDRPRFHEAVLQL
                     DRPAAQVIAELASRGIVAGFDLSNDYPELGNALLVCATETRTDADIKAYADALAETLK
                     AAVRAA"
     gene            4822..6312
                     /gene="gcvPB"
                     /locus_tag="VEW08_04710"
     CDS             4822..6312
                     /gene="gcvPB"
                     /locus_tag="VEW08_04710"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HYJ40067.1"
                     /translation="MAMQKHPEKIIFEYSRPGRGAFGQWPTERDTPNEIAADIPENLR
                     RKKPAMLPEVSELEVVRHFTKLSQLNFSIDTHFYPLGSCTMKYNPKACNSFAMLPGFL
                     NRHPLAPESLGQGFLACMFELQEMLKEVTGMQGVALSPMAGAHGEFAGVAMIRAYHRA
                     RGDLARNEIIVPIAAHGTNPATATMCGCVVKEIPVNDEGDVDVEALRAAVGPNTAGIM
                     LTNPSTCGVFERRIKEVAKIVHDAGGLLYYDGANLNAILGKVRPGDMGFDVIHMNLHK
                     TFSTPHGGGGPGAGAVGVSSRLLPFMPIPVVGKDGDKYRWLTEKDLPQSIGRLSAFMG
                     NAGVLLRAYIYMRMLGREGMHRVGEYATLNANYLMARLAKAGFDPAYPTRRASHEFII
                     TAKKQKNDLGVSAMDIAKRLLDYGFHAPTTYFPLLVPECLLIEPTETESKEALDGFID
                     AMVSILKEAETQADLVRTAPHTMTVRRLDDVRAARQLDLTWKPAAA"
     gene            6445..6819
                     /locus_tag="VEW08_04715"
     CDS             6445..6819
                     /locus_tag="VEW08_04715"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HYJ40068.1"
                     /translation="MNDVGDRQKPTGFTRMFRAFGVSMKGLSGAFREEAAFRQELALA
                     VVVIPLGLWLGHDGIERALLVAPMFLVLVVELLNSAIEATVDRIGQERHQLSGLAKDI
                     GSAAVFLSLLLLAVIWILILWR"
     gene            6846..>7377
                     /locus_tag="VEW08_04720"
     CDS             6846..>7377
                     /locus_tag="VEW08_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HYJ40069.1"
                     /translation="MHPRLLLIGLILLAPAFMQRAGAQNAPPQLVSQSSATQAALPQT
                     EGESAAWRGTLARVAESVVAIEIDQTRAFDTEWNSSAQATGFVVDAERGLILTNRHVV
                     TPGPVTSEATFLNREEVQLYPVYRDPIHDFGIYRYDPKKLRFIRPRSLPLFPDGAQIG
                     REIRVIGNNAGEQLSIL"
BASE COUNT         1349 a         2359 c         2370 g         1299 t
ORIGIN      
        1 cagcggatag tcgcagcgtt cgccgaggcg ccggtagatg gcgatgagat cctgcacgcc
       61 ggacaccttg gccgagagga tgattcgctc gcgcggcagg cccagttcca cagcgcgcgc
      121 agcactctcg agcgccgacg ccaccacggc ctcgcgcatg atttcctggg ccgacaacgg
      181 cgctgcgcgc gaattgttct cgtccatcat gcgtgtgagc aggtcctgat cgaggctgcc
      241 ccagttcacg ccgatgcgaa ccggcttccc gtacttgcac gcgaattcga tcatctcggc
      301 aaattgcgga tcgcgcttgc tgccgcgccc gacgttgccg ggattgatgc ggtacttggc
      361 gagcgcttcc gcgcaagctg gatgttcgcg cagcagcttg tgaccattga aatgaaagtc
      421 accgatgatc ggtacgtcca cgcccatctg cgcgagacga tcgcggatcg gcgcgacggc
      481 ggcagccgcc gacgcttcgt tgacggtgat acgcaccagt tcgctgccgg cgcgcgccag
      541 atccttgatc tgccgcgcgg tgccctcgat gtccgccgtg tcggtgttgg tcatcgattg
      601 aatgacgatg ggcgcagggc caccgacggt gatgcgcccg actcgaacgg caacggattt
      661 gcggcgggga tggctcatgt cttgggcttg ggctggagca gcgtcgagct ctggttcgtc
      721 atctgccagc ggtcgtcccg ccaggcgagc acgacgagaa agtacgcgtc gacgttgcgc
      781 tcggtgccgt cgccgatgga gacggcgtac accttgccgg tcagcaccgc ggaatccgcg
      841 aacagcgtca cgcgttcgac cttgggcacg agcgaacgga acttgcgcgt gccgctgcgg
      901 accgactcga ggtattgggc cttggtggag cacgtggcgc tcacgccgca gtactcgagg
      961 tcgtcggcca gcagcttgtc caaagcggcc acgtcggcgt gcagcgccgc gtcgaaacgc
     1021 gcctgcagcg cggcacgcgc ggagtcctcg tcgccacgcg gggaggccag cgcgatgagt
     1081 ggcagcgagg cgagcagcag caacaggaag cgcggatggc tcatctaact atttcaccgg
     1141 cgagtcgggc atgcgcatgg agcgccatgc cgtcatctgc caacgcccgt ctttccacag
     1201 ccagatgtcg gtgtagccga tgtgcaggtc ctggggctgg cggtccttcc cgctgaccgt
     1261 gaccctggct ttgccacgga tcacgccgac gtcgccgaag atgcgcacgg aatcgacggt
     1321 ggccacgctc gagatgtaat cgcgcttgcc ggtcaccagc gagtcgatga acgaggcctt
     1381 ggtatccaga tcgccattgg agtgggtgta ttcgagtccg tcgtcgagca ggtttccaag
     1441 gaccttggcg tcggcgttca cctgtgcctc gaagcgggtg atctcggccg ccttcaacgc
     1501 tgcctcgcga acgccgtcct tcgcgaagga agcgatggac atcattgttg caactgcaaa
     1561 taggactgct ggggcggcga tgcggcgcat atggatgaat ctttcggctg ggccgtgcgg
     1621 aacggggctg ctatcatgcc acccgctcgc taattgacca gactctgtcc gagagaccct
     1681 ctttaatgag ggcgccgaag gcgcaagttt ccgcccggaa acgctcaggc aaaagaacgg
     1741 cagggtccta cagcgagttc tggagagcga tggccctttt caacgaatcg gccatccacc
     1801 gaaggggtgc ggcgcgcggt ccaacgcgag cccgatctct caggttcaag gacagagggg
     1861 cggcgggaaa ggggaaactc ccttttcccg ccgccttttt gttttggcgc gcactgcggg
     1921 cgggccgagg agatgttctt gggcagacgc acacctttat atgacctgca tgtggggcag
     1981 ggcgcccgca tggtcgactt cggcgggtgg gacatgcccg tcaattacgg ctcccagatc
     2041 gaagagcatc atgcggtgcg gcgcgatgct ggcattttcg atgtgtcgca catgtgtgtg
     2101 atcgatgccc gcggcccccg cgtccgcgaa ttcttctcga aactcatcgc caacgacgtc
     2161 gccaagctca cgcaatccgg caaggccctc tacagctgca tgttgaatga ggccggcggc
     2221 gtcatcgatg acctcatcgt ctactttctg accgagagct ggttccgcgt ggtggtcaat
     2281 gccggcacgc gcgacaagga cctggcctgg atgcgcggga tcgcgccgca gttcggtgtc
     2341 gaactcacgg agcgcacgga tctggcgatg ctggccgtgc agggcccgaa cgcacgcgcg
     2401 aaggtggccg agttgctgcc cgccgagcac cgcgaagcgg cgctcgcact cgaagttttc
     2461 tttggccgcg aactcgggcc ctggttcgtc gcgcggaccg gctacaccgg cgaagacggt
     2521 ttcgaaatca tgctgccggc tgacgaagcg gcacgcgtgt ggcgcaccct caacgagcgc
     2581 ggcgtgaaat ccgcaggcct cggcgcgcgc gatacgctgc ggctcgaggc cgccatgaac
     2641 ctctacggca atgacatgga cgagaaccag aacccgctcg agtcggggct cgcctggacg
     2701 gtgtcgttcg agaacccggc gcgcgacttc gtgggccgga gggcgctcga atcattgaag
     2761 tccgcgggcc tggagaagaa gttcgtggcg ctgttgctcg aagaccgcgg cgtactgcgc
     2821 ggccaccaga aggtggtaac gggcagtggc gacggtgaga tcaccagcgg cacgttctct
     2881 cccacgatgg agaaatcgat tgcgctcgca cgggtacccc gcggcaccgg cgccgcggtg
     2941 caggtcgacg ttcgcggcaa gttattgaac gcgcgcgtgg tgaaagcccc gttcgtgcgt
     3001 cacggcaaga tactcgttag ttagtcccta tttccggagc attttcatga gcaacatccc
     3061 ggcggatctg aagtacacca agtcgcacga gtgggttcgc acgctcgcca acggcaacgt
     3121 cgaagtcggc atcaccgatc atgcgcaggg cgcgctcggt gacctggtgt tcgtcgaagt
     3181 gccggaagcc ggcaaggcgc tcaaggccgg cgacagcttc gccgtcgtcg agtcggtgaa
     3241 ggccgcctcc gatgtgtatg cacccatcgc gggcgccgtc gtcgaaggca atgccggctt
     3301 gagcagccag cccgagttga tcaattccga gccgtacggc gcgggctgga tcatgcgtct
     3361 caagcccggc ggtgcgccgt ccggattgct gagtgccgcc gaataccagg cgcatctcga
     3421 cgcggagggc tgatccatgc cgttcaatcc gcacaccccg aaggatgtga gcgacatgct
     3481 cgccgccatc ggcgcgaaga aaatcgaaga cctgttcgac gaaatcccga aagagctgcg
     3541 catcgagtcg ctggccggca ttccggatgc attgaacgaa atgcacaccg gcaagctcat
     3601 gatggcgcgc gcggcacagg acgggacgcc gctcaatttc atcggtgcgg gcgcctatga
     3661 gcaccacata cccgccgccg tgtgggccgt gaccacgcgt ggcgagttct acagcgccta
     3721 cacgccgtac caggcggaag cctcgcaggg cacgctgcag ctcatctatg aataccagac
     3781 gatgatttcc tcgctgaccg gcatggaagt ctcgaatgcg tcgttgtacg acggcggctc
     3841 ggcggtcgcc gaggcctcgc tcatggcgat acgggcgcat cgcaagagtc ggtcgcagcg
     3901 catcctcgtg ccgaccaccg tgcatccgaa ctatcggaag gtggcagtgg ccacagccac
     3961 gggtcagggc gttcgcttcg aagagatcgc ctgcccgaaa gggcagctgt ccgtcgcgga
     4021 cctgaagaaa tacgaaggcg aggacatcac cgccatcgtc atccagcaac cgaacttctt
     4081 cggcgtgctc gaagacgtgg acgccatcac cgactgggcg cacgccaacg gcatcatggt
     4141 catcggctcg gtcaatccca cctcgctcgc catcctgaaa cctccgggcg agtggggcgc
     4201 gaagggcgcc gacatcgcct gcggcgattg ccagccgctc ggcgtgccgt tgtcttcggg
     4261 tggtccgtat gccggcttcc tcacgacgcg catggaatac gttcgccaga tgcccggccg
     4321 catcgtcggc cgcacggtcg atgccagcgg gcaccagggt ttcgcgctca cgctccaggc
     4381 gcgcgagcag cacatccgcc gaggcaaggc cacgtcgaac atctgcacga accagggatt
     4441 gctggtcacg gccgcgacga tctacctgag cctgttaggt cccgaaggtc tcgcgcgggt
     4501 ggcgaatgaa tcgcaccggc gcacgaacga gctggtggcc gcgctcacgc gcatcaaggg
     4561 cgtgaaactc gccttcgatc gcccgcgctt ccatgaagcg gtgctgcagc tcgatcgccc
     4621 cgcggcccag gtgatcgccg agctggcgag ccgcggcatc gtcgccggct tcgacctgtc
     4681 gaacgactac cccgagctcg gcaatgcgct gctggtctgc gccaccgaaa cgcgtactga
     4741 cgccgacatc aaggcgtacg ccgacgcgct cgccgaaacc ctcaaagccg ccgtgcgcgc
     4801 ggcttaaggg agagaactca catggcaatg caaaaacacc ccgaaaagat catcttcgaa
     4861 tactcgcgtc ccggtcgcgg cgcatttggc cagtggccca cggaacgtga cacgcccaat
     4921 gagatcgccg cggacattcc ggaaaatctt cgccggaaaa agcccgcgat gctgccggag
     4981 gtgagcgagc tcgaagtggt gcgccacttc accaagcttt cgcagctcaa cttctccatc
     5041 gacacgcact tctatccgct gggttcgtgc acgatgaagt acaaccccaa ggcgtgtaac
     5101 tcgttcgcga tgctgccggg tttcctcaat cgtcatccgc tggcgccgga atcgctgggc
     5161 cagggattcc tcgcctgcat gttcgagctg caggagatgc tcaaggaagt cacgggcatg
     5221 cagggcgtcg cgctctcgcc catggcggga gcacacggcg agtttgccgg cgtggcgatg
     5281 atccgcgcct atcaccgcgc gcgcggtgat ctcgcccgca acgaaatcat cgtgccgatc
     5341 gcggcgcacg gcaccaatcc cgccacggcg accatgtgcg gctgcgtggt gaaggaaatc
     5401 cccgtcaacg atgaaggcga cgtcgacgtc gaggcgctgc gcgcggcagt gggtcccaac
     5461 accgccggca tcatgctcac caatccttcc acctgcggcg tgttcgaacg gcgcatcaag
     5521 gaagtggcga agatcgtgca tgacgcgggc ggcctgctgt actacgacgg cgccaacctc
     5581 aacgccattc tcggcaaggt gcgccccggc gacatgggct tcgatgtcat ccacatgaac
     5641 ctgcacaaga ccttctccac gccgcatggc ggcggtggac cgggtgccgg cgcagtaggc
     5701 gtgagctcgc gtctgctgcc gttcatgcca attcccgtgg ttggcaagga cggcgacaag
     5761 taccgatggc tgacggagaa ggatctgccg caatccattg gccgcctctc ggcattcatg
     5821 gggaacgcgg gcgtgctgct gcgcgcgtac atctacatgc gcatgctcgg acgcgagggc
     5881 atgcaccgtg taggagagta cgcgacgctc aacgccaact atctgatggc gcgtcttgcg
     5941 aaggcgggct tcgatcctgc gtatccgacg cgtcgcgcca gccacgagtt catcatcacg
     6001 gcgaagaagc agaagaatga tctcggggtc tcggcgatgg acatcgccaa gcgattgctc
     6061 gattacggat tccacgcgcc cactacctac tttccgctgc tggtgcccga atgcctgctc
     6121 atcgagccca ccgaaaccga gagcaaggaa gcgctggacg gcttcatcga tgccatggtg
     6181 tcgatcctca aggaggccga gacccaggcc gatctcgtca ggacggcgcc gcataccatg
     6241 acggtgcgcc ggctggatga cgtccgcgcc gcgcgtcagc tcgatctgac ctggaaaccc
     6301 gcagcggcct gaagtacgcg ctcttcactt tgacggacgc cggcgcttcg ccggcgtttt
     6361 cctttgtggg gcgcagcccc tggcaatacg gcgatttccg ttgtggggcg cagcccctgg
     6421 caagtgcctg ataatatcgc ccgcatgaac gacgtaggcg accgacagaa acccaccggg
     6481 ttcacgcgta tgtttcgcgc cttcggcgtg agcatgaagg gactgtcagg ggcgtttcgt
     6541 gaagaggccg cctttcgcca ggaactcgcc ctggcggtgg tggtcattcc actgggcttg
     6601 tggttaggtc acgatggcat agagcgagcg ctgctggttg cgccgatgtt cctcgtgctg
     6661 gtcgttgagc tgctcaatag cgccatcgaa gccaccgtcg accgcatcgg tcaggagcgt
     6721 caccagcttt cggggctcgc caaggacatc ggatccgcgg cggtgttcct ctcactgctg
     6781 ctgctggcgg tgatctggat tctgattctc tggcgctgat gcctaacaag acgaaaggac
     6841 gacgcatgca cccacgtctg ctgctcattg gcttgatcct cttggctccg gccttcatgc
     6901 agcgggcggg ggcacagaac gcccctcctc agctcgtgtc gcaatcctcg gcgacccagg
     6961 cggcattgcc gcagaccgaa ggtgagagcg ccgcgtggcg tggaacactc gcgcgagtcg
     7021 cggagagcgt ggtcgccatc gaaatcgacc agacgcgggc cttcgacacg gagtggaatt
     7081 cttccgcgca ggccaccggc ttcgtggtcg atgccgaacg cggcctcatc ctcaccaatc
     7141 gccacgtggt cacgccgggc ccggtgactt cggaagccac cttcctcaat cgcgaggaag
     7201 tgcagctcta cccggtgtac cgcgacccga tccacgactt cggcatctac cgctacgatc
     7261 cgaagaagct gcgcttcatc aggccgcgct cgctgccgct gttccccgat ggcgcacaga
     7321 tcggccgtga aatccgcgtc atcggcaaca atgccggcga gcagctctcc atcctcg
//
LOCUS       DASQJS010000118        27295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1949395, whole genome shotgun sequence.
ACCESSION   DASQJS010000118 DASQJS010000000
VERSION     DASQJS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 27295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27295
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949395"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VEW08_04725"
     CDS             complement(<1..304)
                     /locus_tag="VEW08_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the transfer of an amino moiety;
                     preference for methionine followed by histidine and
                     phenylalanine; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HYJ40070.1"
                     /translation="MIFPEGIQSKLPDVGTTIFTVMSRRAAEQGAVNVGQGFPDFPID
                     PQLAACVNDAIAAGFNQYAPMQGSVALRTAIAAKILAAGGRAVDPETELTVTCGGTE"
     gene            complement(363..599)
                     /locus_tag="VEW08_04730"
     CDS             complement(363..599)
                     /locus_tag="VEW08_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40071.1"
                     /translation="MAKSARKHNAPWTPADLKKMRQLAKDKYSARLAAKELGRSTGAV
                     KYKAMVEGIRFRFIDQPAGVQLKLARKAKRAKKK"
     gene            complement(646..1500)
                     /locus_tag="VEW08_04735"
     CDS             complement(646..1500)
                     /locus_tag="VEW08_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYJ40072.1"
                     /translation="MKTHGWFSKTALGLGFAFLYVPILSMIFYSFNYSKLVTVWDAAR
                     SPTLKWYAALFHDNMVLRAAWLSIEVAALSATGAVILGTLAGLVLSRFGPFRGRALLQ
                     GLTTAPLVMPEVITGLSMLLLFTALEPLLLNWFGYTLNRGISTITIAHITFTMAYVTV
                     VVQSRLSSFDDSLEEAALDLGARPAKVFFRITVPLILPAILSGWLLAFTLSWDDLVVT
                     QFVSGPGSNTLPMVIFSRVRLGVSPVVNALATIMVLIVALGVVLSAVFMRRQEQRRKR
                     EEQMAAAG"
     gene            complement(1511..2518)
                     /locus_tag="VEW08_04740"
     CDS             complement(1511..2518)
                     /locus_tag="VEW08_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYJ40073.1"
                     /translation="MKTAGRNVAARDSMGRLGPLWSIFFRFGPPRWSQYVVTNWGGQR
                     LVIAVPYLWLLVFFLIPFLIVLYISFAGPADEQSKLPYAPVITWVAEGGTQIKLLFTS
                     YSYLIHDPLYVSAWGYSIKLAAVSTLFCLLVGYPMAYAISRSTPTMRNIFLMLVILPF
                     WTSFLLRVYAWIGLLKANGVINNTLMWLGIIDTPIGMMNTTFAVYIGIVYSYLPFMIL
                     PLYSNLEKHDNTLLEAANDLGAGPIRSFLRITLPLSKDGIVAGSLLVFIPAVGEYVIP
                     TLLGRTDQLMIATLLYNEFDRNRQWPTACAVAIAMLLVLIVPIMIFQHFQNKELKAGR
                     K"
     gene            complement(2540..3706)
                     /gene="potA"
                     /locus_tag="VEW08_04745"
     CDS             complement(2540..3706)
                     /gene="potA"
                     /locus_tag="VEW08_04745"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HYJ40074.1"
                     /translation="MQSAERRADKNKTTKNEPWRDPHAEAYVRIDNITKRFGDFVAVN
                     NVSLKIYKGEIFCLLGGSGCGKSTLLRMMAGFEEPSSGAIFLDGQDMAGIPPYRRPVN
                     MMFQSYALFPHMTVEKNIAFGLEQEKGLSSADIATRVTDILEIVKLGQFAARRPHQLS
                     GGQKQRVALARSLVKRPKLLLLDEPLGALDKKLREATQFELINIQEQLGVTFIVVTHD
                     QEEAMTLASRIGVMNRGEIVQIGTPTEIYEFPQNKFVADFIGSVNMFEGTMIEDLPDR
                     VRIKSDELGGIIYVDHGISAAPGAIVYTAIRPEKINILRAQPAETHENCVKGVVKDIA
                     YMGDVSIYLVKIDSGKIVRVTLQNAERLSDDERIGWEETVWLTWHGSSPVVVTQ"
     gene            complement(3781..4938)
                     /locus_tag="VEW08_04750"
     CDS             complement(3781..4938)
                     /locus_tag="VEW08_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HYJ40075.1"
                     /translation="MSTRSLSALVSASCLFLLIACGKKEEAPPPQAEAAPPAAVFDTD
                     AEKVVNVYNWSDYIEPKVLEEFETETGVHVNYEVMDSNALLETKLRAGRSGYDVVVPS
                     ASYLARQIKSNIYQKLDKSKLDNLKNLDADITKRLEVFDPGNEHAVNYMWGTSGVAYN
                     EGLIKAAMPDAPVDSFAMFWDPKVVSKFAKCGVSILDEPGEVLGTVLMYLGKDPNSES
                     PEDLKAAEKVLLSVRPYVRLINSSKYIEDLANGEICLALGWSGDTLQAKSRADEAGKG
                     LTIKYNIPKEGAVMFFDNMAIPADAVHVKNAHLFINYMLRADVAAKNSNFISFANSNA
                     ASWPLVSAEVKSNPGIFPTPEMMPKLVPDLPESAEFTKQLTRTWTRFRTGK"
     gene            complement(5017..6402)
                     /locus_tag="VEW08_04755"
     CDS             complement(5017..6402)
                     /locus_tag="VEW08_04755"
                     /inference="COORDINATES: protein motif:HMM:NF005682.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HYJ40076.1"
                     /translation="MNTSPETKEWQRLDSAHYLHPFTDHKQLAAKGARIITRAEGVYI
                     YTSEGDKILDGMSGLWCVALGYGRKELSEAAYEQMLELPYYNSFFQSATQPAIELARI
                     LKEVTPPQFNHVFYTGSGSEANDTLIRLVRRYWQLLDQPERNVIVSRWNGYHGSTVAG
                     ASLGGMKPMHDQLGPNGALPGIVHINQPYWFGEGGDLSPEEFGLKCARELEAKILEVG
                     ADRIAAFIGEPVQGAGGLVIPPETYWPEIQRIVDKYGILFASDEVICGFGRTGQWFGC
                     EHYGTRPDFMTLAKALSSGYLPIGGLMVGDRVANVLIDKGGEFFHGFTYSGHPAACAV
                     AIANLEIFRRERIVERVREETAPYLAQRWKELLAHPLVGEARSVGLLAAIELVPDKRT
                     RRFFEPRGEVGVRMRDNCTRNGLVMRATRDTLFVAPPLVISRSEIDELLSKVVRSLDE
                     TAIQARAEGWL"
     gene            complement(6399..7739)
                     /locus_tag="VEW08_04760"
     CDS             complement(6399..7739)
                     /locus_tag="VEW08_04760"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HYJ40077.1"
                     /translation="MANEIRQFLRDHGISEVEAIIPDMAGIARGKIMPAEKFAEDEGM
                     RLPESIFLQTVTGDYPPDTDKAMSPAEIDIILKADPKTVRRVPWAAEPTAQVIHDCFY
                     SDGRRVGMAPRGVLRHILEMYANRGWEPVVAPELEFYLVEPNIDADYPLKPPVGRSGR
                     AEPGRQSYSIAAVNEFDPLFDDMYQFCEDQDIEIDTLIHEDGAAQMEINLLHGNALDL
                     ADQAFLFKRTAREAALRHKMYATFMAKPHAKEPGSAMHIHQSIVDIKTRKNIFSNGDG
                     TPTPLFFSHIAGMQKYLPAAMALLAPNVNSYRRISRFQLAPINVQWGYDNRTAGLRVP
                     SSDPEARRVENRLAGADANPYIAIATSLACGYLGMVEGLQPSEPISGSAHELPFSLPR
                     SLDDAIRELRECEPLVRILGDPFISAYTIVKEAEYEVFLQVISSWEREHLLLNV"
     gene            complement(7745..8530)
                     /locus_tag="VEW08_04765"
     CDS             complement(7745..8530)
                     /locus_tag="VEW08_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HYJ40078.1"
                     /translation="MASSTHFSKRPAYRPVIGIPADRRQIGLHPFHAVGEKYIAAIVQ
                     AADCLPLLIPVLESPLDVSEILARVDGIFFPGSPSNVEPARYAGPESVPGTLHDPHRD
                     ATTLSLIPRAVEDGVPVLGVCRGFQEMNVAMGGTLHQRVHEVSGLDDHREDKEQPLEV
                     QYGPAHDVVLEPGSVLRGLSGQERVRVNSLHWQGVDRLGPGLAVEARATDGLVEAFRV
                     AKAPAFALAVQWHPEWQVMSNPFSKALFAEFGKAARNRTKVVR"
     gene            8780..10144
                     /locus_tag="VEW08_04770"
     CDS             8780..10144
                     /locus_tag="VEW08_04770"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HYJ40079.1"
                     /translation="MTRAADPTLERFLADHPKIRFFDAFVNDLNTVERGKRIDRAGIA
                     TVFERGMPLPGSMFALDIEGGTVEDTGLGFNDGDADRPCMPIPGTLVPVPWQPDVAQV
                     QLRMFDHDGTRFFGDPRWILENHLELFKALDLTPVVAIEYEFYLVDAERSAAGLPQPP
                     RGPLTGRREYRSQINSMADLNEFSPLLSEIDRVCHEQNVPTTSALAEYGPGQYEVNLA
                     HGPDALRACDEAVRFKRIVKSVARKHGCDATFLPKPYRDMAGSGLHIHVSLLDSSGRN
                     VFAAASAEQSPKLRHAIAGTLASLGDGMAICAPGPNSYRRFRPEAYVPMHPTWSVNNR
                     GSAVRVPASDAANLRLEHRLAGADANPYLVTAWILAGIHHGLTRGLEPPPVTTGNAYE
                     QSGEPLPIVWSEAIERFAASGFAKQYFGEKFTHLYSTVKRAEAQEFNSHITPLEIARY
                     LGPL"
     gene            10155..11438
                     /locus_tag="VEW08_04775"
     CDS             10155..11438
                     /locus_tag="VEW08_04775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYJ40080.1"
                     /translation="MTGAESNYYFATSPPPPPHVPLRGEIRADACIVGGGISGLSAAL
                     HLIERGFSVALLEAKHLGFGGSGRSGGQSIFGYASSQEKLEKLVGETDARFMWDIALE
                     GMKLQRELIARHSIGCDYVAGHMHVGVKSRHDAELRAEVESLHSKYGYRSVRLVGHEE
                     LRTIVQSDRYTTGIYDANSGHLNPYKYTLGLGQAAARAGVRIFENSRVTKLELAARAD
                     ADNLVHTESGTVRARYLLIAGNALLGRLVPALSRKLMAVGTYIAATEVLGEERARALI
                     TNNAAVADINWVLDYFRLSADYRLLFGGRVSYSGVDPFDSARVLRARISRVFPKLNPR
                     IDYAWGGYVDITPNRAPHFGRLAPNVYFLQGFSGHGMVLSGIAGKLVAEAMAGVAARF
                     DVFAKIPHRDFPGGTYFRRPALVLAMLWFRLKDLL"
     gene            11435..12214
                     /locus_tag="VEW08_04780"
     CDS             11435..12214
                     /locus_tag="VEW08_04780"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HYJ40081.1"
                     /translation="MSGFDVGVWLSALPVLLIAATVTWLLSLSMRNVSIVDSLWSLML
                     FAAGVIYALGADPRAPRLAFVLWMLAIWAARLSLYITVRNMGKGEDRRYRAIRERNEP
                     HFAFKSLYLVFWLQALLAWIISLPLLGAFSSIAPIGILDYLGLILWLTGFVFEALGDW
                     QLARFKRDPVNADAVMTSGLWRFTRHPNYFGEFCIWWGFAAVAVSAGGWWSLPGPALL
                     TVLLLRVSGVSLLEKDIGNRRPRYADYVLKTNAFFPGPTRK"
     gene            12292..14163
                     /locus_tag="VEW08_04785"
     CDS             12292..14163
                     /locus_tag="VEW08_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ40082.1"
                     /translation="MIEVDSAQCEARAREVRHDVVATFTLATHRIFRYVFAVQWAIAV
                     AMAWQSTLPGEPRVVFTMIVGAMLSVPAVLFARAAPQAAWVRHYTAASQIGWSILFMW
                     LLEGQPESQLHVFIALAFISFYRDWRALMTATFIALAFPIVRLALLPESYTIGAAAWW
                     RVFDHATWVFSDAVIMLLAVLQSLKTIQKFALHSATLTLTNETIGKHVEERTRELARS
                     REQYRLIAETTRAIPFELDLANGRFTYIGPQAQKMLGFPESQWKEPEFLDVLLPRSRE
                     SNVRQQIDECTPGTFETLCSVVTADNRVVELRWTVSCELVNGMRFLRGLMIDVTEARR
                     MVRELAQGQKLESVGRIAAGVAHEINTSVQFISDSVRYVRHALRDVPHALADYRALAA
                     SALSGGDVTAAAKKAADTDEAADVDYFLKNAPDALDRALEGIGRMGSVVRSMTEFAHP
                     DSRTKADIDLNRTIRTTLNMARNEYKSVAELETDFGDIPAVHCHAGDINQVVLNLVLN
                     AAHALGDRAAGSMSKGRIAVRTRAIGGYVEISIRDTGDGIPEDVRGRIFEPFVTTKEV
                     GRGAGQGLALSRGIVVDKLKGSLHFETETGKGTTFFIRLPVSDTALATDSNNKQAAA"
     gene            14160..15392
                     /locus_tag="VEW08_04790"
     CDS             14160..15392
                     /locus_tag="VEW08_04790"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ40083.1"
                     /translation="MTSLHVLFVDDEQAILDGLRNSLRKERKRWDMTFALGGQQALEE
                     MKKTPADVVITDMRMPGMDGAELLRHIQRDFPAAARIVLSGQAERESIMRALPVTHQF
                     LHKPCDGETLRAVIEQTHALHKLMQNPALTALVGKLERIPAVPSTYTELSRIAADPKA
                     DSAMFAKVIEMDAAVCAKVLQLVNSAYFGLGQKIVSIRPAVTYLGVEIIKSLVLSSTS
                     FSDKAISEVKGFSPDRLQQHSMLTAILAKKIVSNPALADAAFTAGLLHDIGALVLLHA
                     APPDYVRALERKKELNGDSAAAEREIFGVTHAEVGAYLLGLWGIPFPIVEAVAFHHRP
                     NEVAPESRPLVAAVHIASGLVEEMTESNGAQVLGGTSPDSSARIDVSFLREAGLEELF
                     KKSRTEAEKLVGGVAERS"
     gene            15433..16494
                     /locus_tag="VEW08_04795"
     CDS             15433..16494
                     /locus_tag="VEW08_04795"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HYJ40084.1"
                     /translation="MRIARITAVVLSLTAGLAGALLVSTTDAAGTPGAPTSAAAASAF
                     LKSMPADLRASASFPLEAPERLVWNFVPMDRVGVSLLKLDDAQSELLGPLLATALSPE
                     GLLAARGVMKHENILRRVETEAGVANASRRDPGLYYTAIFGKPAPAAAWGWRFEGHHL
                     SINVTELPGESPVVAPMFLGANPARVLAGPNAGFRLLAAEEDLGRELIKMLPASRRES
                     ATIRDTAFSEILTGNDPKVQKLELEGLAAAEMTAEEKAQLRRLIELYTGRMTPASARD
                     ALSRLDRAGFDKVRFAWAGGIEPGQPHYYRVHGPTLLIEYDDTQNNANHIHTVYRDLE
                     RDFGGDALRAHYRGALSRP"
     gene            16561..17070
                     /locus_tag="VEW08_04800"
     CDS             16561..17070
                     /locus_tag="VEW08_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HYJ40085.1"
                     /translation="MGKKNTTSAATMAAPLIDIGIKGKDREKIVQGLSRVLADTYTLY
                     LKTHNFHWNVEGPMFNTLHLMFMDQYTELWNALDAIAERVRPLGYPAPATGKEFSKLT
                     SIEETEGVPDALDMVRLLVAGHEAVTRTARSAFPAAEKAGDESTCDLLTQRLQIHEKT
                     AWMLRSLLK"
     gene            17067..18026
                     /locus_tag="VEW08_04805"
     CDS             17067..18026
                     /locus_tag="VEW08_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYJ40086.1"
                     /translation="MRVPISRIAALLAAAAILFAGATAGAAKLKVALTFDDLPLNGTL
                     PTGKKQSDFARDTIKVLKKHRIPPSYGFINANKLERNPDGAQALQIWAKGGHPLANHT
                     YTHLDLTKNSVEDFQREILRNEPALELLMPSSSQDSGGKHGRWSWFRYPYLHEGDTLE
                     KRRAVRAFLASSGYRIAQTTIDFEDYLWNSAHARCWMKKDAEGLDWLRESYRTAAREF
                     IRLDIANSRAAFGRDIHHVMLLHLGSFSSHILPDLFDILDEEGFEIVTLEEAQKDPAY
                     DYDPDVADPRGGTLVELAMKAKNIPWPANAPKKPRDRLAAICN"
     gene            complement(18036..19082)
                     /locus_tag="VEW08_04810"
     CDS             complement(18036..19082)
                     /locus_tag="VEW08_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYJ40087.1"
                     /translation="MSQTLGFGFIGAGAIANFHARAVAASQGGHLVGVVSRTRATAEK
                     FAAEHGMGFASDDVAQLLAQPNLDAVCISTPSALHLVPALAAIRAGKHLMIEKPLDST
                     VEGTDHILHEAAKAGVRVGSIFQARFGDAARSLKAAVDAGRFGRMVLASCYVKWNRTP
                     GYYTGWKGRISQDGGGAVINQAIHGVDLLQWFAGMPVEVFAWTTQRVHAIESEDTSVA
                     ALRFGSGAFGTIEASTALWPGFSRRIEICGENGSAVMEDDDIASWEFRVAAPEDEAIR
                     RARETSAKGSGAANPMAINFEGHRRQIQDFIDGIREQRPFFIEGSEARKAVALVRAIY
                     DSAASGAPVRPDGV"
     gene            complement(19104..20417)
                     /locus_tag="VEW08_04815"
     CDS             complement(19104..20417)
                     /locus_tag="VEW08_04815"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYJ40088.1"
                     /translation="MKFFKLAASVIITGVLGSAVAADAPKPAPASPPTPVVPVPPPPP
                     LVDPAAIDSALKSIIDGKKAVGVSALVYERGREAYFGAFGLSDRENNKPMTRDTIVQI
                     FSMTKPVTGVALMRLYERGKFDLDAPLSVYAPEFADVKVYAGLDANGQPKYEAPKRPI
                     TVRDILRHTAGFHGDGSPEAVTAIYRQIDPRNLDNALPEVSARLAKVPLSYQPGTQWK
                     YSDVVDVQAYLVEKISGVPFDEFLMLHILRPLGMTSTRYTILPTDADRPQLAAIYTRD
                     GGTLTRQTDEEAYRFNSAAWPQKPGSYGLVSTIDDYMKFARMLAGSGKLGRARVLKPE
                     TVRLMATDAMPKEVTDKGWLPNKGQVGFGIDFAVRIAPPKDAQEPSGAVGEFFWDGAA
                     STLFWVDPKNDITAVFFTQVRPFDGSLHKIFRDAVYRNDPIALAH"
     gene            complement(20414..21235)
                     /locus_tag="VEW08_04820"
     CDS             complement(20414..21235)
                     /locus_tag="VEW08_04820"
                     /inference="COORDINATES: protein motif:HMM:NF045039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40089.1"
                     /translation="MAAAPAVATLLFVLCPSSSPAADHPILFTDDFESHATAEFPGAP
                     WSEATFKSGAVISVDADHAYSGHQALHIFTPRGANYRRGYVAIHLGTPVPAAMSGMFG
                     RAMVFLDEAPEAVPGGTAVHWTLLQGEGRSADDRYNAIYRLGLEGKGGTQLMANFETT
                     PPVTTDCKQNSDRTLPVKRWTCVEWHMEVASNEMQFWLDGRKVVHVAGRATAAKACRG
                     NDLQGEWRAPPKFDSFYIGFERYADSANDQNLWIDDVVLSKARVGCSTPKRGGDR"
     gene            complement(21268..22218)
                     /locus_tag="VEW08_04825"
     CDS             complement(21268..22218)
                     /locus_tag="VEW08_04825"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HYJ40090.1"
                     /translation="MRFAILGAGALGTILGSHLSRAGHDVVMIARGERARVLQRQGLV
                     LNGLSDIKARPIVVDNPSKLVDTDTLIIATKAIDSANSLDTLKHLRLENAFSVQNGVL
                     KNELLARLFGFSRVLGAMADFSGELLANGEVKFTRNVCLHLGEEKGGSSPRAEALAAL
                     IDSAGVRCAAASNIRTREWSKFAGWIAQFPLAVLTRQITWKFLMDERSAMVIVRIVRE
                     AAALAAAMKIELTDMPPLPVPSIVHGSDAQAIEIVRGIGQKFFETSPEHRMSAQQDVL
                     RGSRLEYEETLGYALEKAREFRVPMPTLEVCYRIIAVAHP"
     gene            complement(22234..24276)
                     /locus_tag="VEW08_04830"
     CDS             complement(22234..24276)
                     /locus_tag="VEW08_04830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYJ40091.1"
                     /translation="MKFRSLAVSATALLFAVCAGAADLPESRRLHSIDVFQLEYADDV
                     QISPDGNRIVYVRVSHDIMTDRARRNLWVVDATGANNRPLRSESRNFSSPRWSPDGTR
                     LAYVSAAEGSPQLYVRWMDSGQTALLTNLVEAPEAIAWSPDGKSIAFTQLVPVKKPPL
                     ATPPEKPEGAQWAPPVKVIDSVIYRADGEGYLEAGYQHVFIVSAEGGTPRQLTEGEFN
                     DGGPLSFTPDSKRIVFSANRGPDWERDPQESEVFDVDIATQKLTQLTTRDGPDNSPVV
                     SPDGKRIAYLGFDDRLQGYQVTHLYVMDTDGKNSRVVTAQFDEDIENPRWAADSRSVY
                     FSYDERGVKKLGVVSLDGKVRNIAEGLGGTDLGRPYTSGMYSVARNGRVAFTHNAPSR
                     PADVATATAGGATKLLTALNDDLFGARALGQVRELNWKSSHDQRAIQGWVITPPDFNP
                     AKKYPLVLEIHGGPFTAYGPNYTTELQLYAAAGYIVLYTNPRGSTSYGEEFGNLIHHA
                     YPGNDYDDLMSGVDALIAEGNVDTSNLFVTGGSGGGVLTAWIVGKTDRFRAAVVAKPV
                     INWSSFVLTSDANNFFYKYWFGAQPWEQPEQYWKRSPLSLVGNVKTPTMLLTGEADYR
                     TPISESEQYYQALKLRKVDTLMVRIPEASHGMTGRPSNLIAKVDNILAWFEKYRKK"
     gene            24366..25769
                     /locus_tag="VEW08_04835"
     CDS             24366..25769
                     /locus_tag="VEW08_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HYJ40092.1"
                     /translation="MRTICWHVFISMGAAMGATSAQAADAAPDREVSLTIYNKNIALI
                     EHVRPISAPAGRQRIEFTGVSAQIVPETVSFIAPNVVLVEQNFDYDLLTPAKLMEKAV
                     GSQVRVVRVNPATGAETTETAEVLSTVGGVVLRIGTRIEVLREDNLPTRVIFDKVPEN
                     LRAQPTLSVLVNAAKPVNEVARLTYLSRGFTWSAEYVALFDETRGTTSMQGWITLRNT
                     SGTSFTNARTQLIAGDVNLVDSEEQWWTRWQQRRSTSQRSAGTESTGRQQLGDYYVYP
                     IRQLTTVANNQTKQVSFIDAENVKASKGYELSFYGFQSNTEPQSAEIRMRIENSKAAG
                     MGEQLPSGVVRIYMRDARGQPQFVGEDLISHTSAASELALKIGDAFDVTVQPTLLETR
                     RITKRKSEFDMSYLVRSARGTPSVVTLRQFGLWRQNEMLKESIVGRRTDANSFAWDVP
                     VPANGETTLTFTVATSW"
     gene            25775..27229
                     /locus_tag="VEW08_04840"
     CDS             25775..27229
                     /locus_tag="VEW08_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40093.1"
                     /translation="MRSTTFIVMALLAGAHSARADTMEVVSPRADAVSVTIYRDLFAL
                     VTETRTVDLPEGPVTLSFDGVVETLLPESAVVTDLGRALEERNFDYDPLSPNSLLARS
                     IGKHVTITRTLPGAGRVVQTRAVIAAANAGGITLRTESGNEALHCSGIPERLTFDEIP
                     ADLNARPTLSIRLAAGPAGKRTIRLSYLAHGFAWKSDYVARLNATGNRMSLTGWITLQ
                     NYTQANLRGAQVQVVAGKLHLVDKQEGGSSLTGDSEDFDDEFRLNQARESALQDLQRE
                     LEPKDSTLQLFSGCHALPVPDTRNDKLSDFASAEIVVTGFRGTMRMSMNMKRDVMTAV
                     REELGDYQLYRLPWATDLNARQTKQAVFLVKPRVKVDRFYSFAFDAGNIEALETYTPE
                     SMFAFDNRKSSGLGEPLPQGLVRMFETAGSGDVFLGESRMDDNPVNVPVELALAQAID
                     LALTIDVDPGDPEELRDANLDERADIFARLCTYA"
BASE COUNT         5029 a         8641 c         8608 g         5017 t
ORIGIN      
        1 attcggtgcc gccgcaggtt accgtgagct cggtctccgg gtcgaccgcg cgacccccgg
       61 cagcgaggat cttggcggcg atggcggtac gcagcgcaac actgccctgc atgggcgcgt
      121 actggttgaa tccggccgcg atcgcgtcgt tgacgcaggc agccagttgc ggatcgatgg
      181 gaaaatcggg gaagccctgc cccacattga ccgcgccctg ttcggccgcg cggcgggaca
      241 tcacggtgaa gatcgtggtg cctacgtccg gcagtttgct ctggatacct tccggaaaaa
      301 tcatcttcgg cagatgagca aagccgccgc gcacccccgt acgcggcgcc ttcgtcggac
      361 gcctacttct tcttagcgcg tttcgccttg cgggcgagct tgagctgaac acctgcgggc
      421 tggtcgatga agcggaagcg gataccttcc accatcgcct tgtacttgac cgcaccggtg
      481 gagcggccca gttctttggc ggcaaggcgg gcggagtact tgtctttggc aagttgccgc
      541 atctttttga ggtctgcggg cgtccaggga gcattgtgtt tacgggctga tttggccatt
      601 gatcggatcc tcgtcaaatt gtcgatggtg gaagtttagc agtagttagc cggcggcggc
      661 catctgttcc tcgcgtttgc gacgctgttc ctgccggcgc atgaaaaccg ccgacaaaac
      721 cacgcccaat gccactatca ggaccattat cgtagctagg gcattgacga ccgggcttac
      781 acctagtcga acacgagaaa atatgaccat gggcagcgta ttggacccag ggccggagac
      841 gaactgcgtc acgaccaggt cgtcccagga aagagtgaat gccagcagcc acccggacag
      901 gatggcgggc agaatgaggg gcacggttat gcggaagaac acctttgccg gccgggcgcc
      961 caggtccagg gcggcctcct ccagcgaatc gtcgaagctc gacaggcggg actggacgac
     1021 cacggtcacg taggccatgg tgaaggtgat gtgcgcgatc gtgatggtgc tgataccgcg
     1081 gttgagcgtg tagccgaacc agttgaggag cagcggctcg agagcggtga acagcaggag
     1141 catcgacagg cccgtgatga cctcgggcat gaccagcggc gcggtagtga ggccctggag
     1201 cagcgcgcgg ccccggaaag ggccaaaccg tgacaacacc aggcccgcaa gcgtgccgag
     1261 gatcaccgcg ccggtggccg acagggccgc gacctcgatg gacaaccacg ccgcgcgcag
     1321 caccatgttg tcgtggaaca gggcggcgta ccacttgagc gtgggcgaac gcgccgcgtc
     1381 ccacaccgtg accagcttcg aatagttgaa cgaatagaag atcatcgaca ggatcgggac
     1441 gtacaggaaa gcgaacccga gcccgagcgc cgtcttggaa aaccagccgt gggttttcat
     1501 ggagcgcctc tcatttcctg cccgccttca gttccttgtt ctggaaatgc tggaaaatca
     1561 tgatgggcac gatcaacacc agcagcatgg cgattgcgac cgcgcacgcc gtcggccatt
     1621 gccggttgcg atcgaattcg ttgtagagca gcgtcgcaat catgagctgg tcggtgcgcc
     1681 cgagcagcgt cgggatgacg tattcgccca ccgccggaat gaacaccagc agcgagccgg
     1741 cgacgattcc atctttcgac agcggcagcg tgatgcgcag gaaactgcgg attggcccgg
     1801 cgcccagatc gttcgcggcc tccagcaggg tgttgtcgtg cttctcgagg ttcgaataca
     1861 gcggcaggat catgaacggc aggtaggaat agacgatgcc gatgtacacg gcgaacgtgg
     1921 tgttcatcat gccgatcggc gtgtcgatga tgccaagcca catgagcgtg ttgttgatca
     1981 cgccgttggc cttgagcagc ccgatccacg catagacccg caacaggaac gacgtccaga
     2041 aaggcaggat gaccagcatg agaaaaatgt tgcgcatggt cggcgtgctg cgcgagatgg
     2101 cgtaggccat cggataaccc accagcaggc aaaacagcgt cgagaccgcg gccagcttga
     2161 tcgagtatcc ccacgcgctc acgtacaggg ggtcgtggat caggtagctg taactcgtga
     2221 acagcagctt gatctgcgtg ccgccttcgg cgacccaggt gatcaccggt gcgtacggta
     2281 gtttgctctg ctcgtcggcc ggccccgcga acgagatgta cagcacgatc aggaaaggga
     2341 tcaggaagaa gaccaggagc cacaggtacg gcactgcaat gaccagccgc tggccacccc
     2401 agttggtgac cacgtattgc gaccagcgcg gcggcccgaa acggaagaag atcgaccaca
     2461 gcggtccgag gcggcccatg gagtcgcgcg ctgccacgtt gcggccggct gtcttcacgg
     2521 cgtcagctct cccggcgcat cattgggtga cgacgaccgg gctggatcca tgccacgtca
     2581 gccacaccgt ctcctcccag ccgatgcgtt cgtcgtcgga caggcgctcg gcgttctgca
     2641 atgtcacgcg tacgatcttg ccgctgtcga tcttgaccag gtagatggac acatcgccca
     2701 tgtaagcgat gtccttgacg acacccttga cgcagttttc atgcgtctcg gccggctggg
     2761 cccgcaggat gttgattttt tccggccgga tcgccgtata gacgatggcg ccgggcgccg
     2821 cgctgatgcc gtgatcgacg tagatgatgc cgccgagctc atcggacttg atgcgcacgc
     2881 gatccggcag gtcttcgatc atcgtacctt cgaacatgtt caccgagccg atgaagtcgg
     2941 cgacgaactt gttctgcggg aactcgtaga tctccgtcgg cgtgccgatc tgcacgattt
     3001 cgccgcgatt catgacgccg atgcgcgacg ccagcgtcat cgcttcttcc tggtcgtggg
     3061 tcacgacgat gaaggtgacg ccgagctgtt cctggatgtt gatgagctcg aactgcgtgg
     3121 cttcgcgtag tttcttgtcg agtgcaccga gcggctcatc gagcaacagc agcttgggtc
     3181 gcttcacgag cgaacgtgcg agcgcgacac gctgcttctg gccgccggaa agctgatgcg
     3241 gccggcgggc agcaaattgc ccgagcttga cgatctctag gatgtccgtg acccgcgtgg
     3301 cgatatccgc gctcgagagg cctttctcct gctccaggcc gaacgcgatg ttcttttcca
     3361 ccgtcatgtg cgggaacagc gcgtacgact ggaacatcat gttgaccggg cgccggtacg
     3421 gcggaatgcc ggccatgtcc tgcccatcga gaaaaatggc gccgctgctg ggctcttcga
     3481 acccggccat catgcgcagc agcgtcgact tgccgcagcc ggatccgccg agcaggcaga
     3541 agatctcgcc cttgtaaatc ttgagcgaaa cgttgttcac cgccacgaaa tcgccaaatc
     3601 gcttggtgat gttgtcgatg cgcacgtagg cctcggcgtg cggatcacgc caaggttcgt
     3661 tcttcgtcgt cttgttcttg tccgcgcgtc gttccgccga ttgcatggca gacccccgtt
     3721 gcaatgcccc ctttcgaggg gattaagggg ccgctgctcc ctaagcggcg gcccccagga
     3781 tcatttgccg gtacggaagc gcgtccaggt gcgcgtcaac tgcttcgtga attcggcgct
     3841 ctcgggcaga tcaggaacca gcttcggcat catttccggt gtcgggaaaa tgcccggatt
     3901 gctcttcact tcggcgctga ccagcggcca cgaggcggca ttgctgttgg cgaagctgat
     3961 gaagttgctg ttcttggcag ccacatcggc ccgcagcatg tagttgatga acaggtgcgc
     4021 gttcttcacg tgcacggcgt cggcgggaat ggccatgttg tcgaagaaca tgaccgcgcc
     4081 ctccttcgga atgttgtact tgatggtcaa gcccttgccc gcttcgtcgg cgcggctctt
     4141 ggcctgaagg gtgtcacccg accagccgag cgccaggcag atctcgccgt tcgcgaggtc
     4201 ttcgatgtac ttcgaggagt tgatgaggcg gacgtacggc cgcaccgaca acaacacctt
     4261 ttcggcggcc ttgaggtctt ccggcgactc gctgttcgga tccttgccga gatacatgag
     4321 gaccgtgccg agcacttcac cgggctcatc gaggatcgag acgccgcact tggcgaattt
     4381 cgagaccact ttcgggtccc agaacatcgc aaagctatct accggcgcgt ccggcatggc
     4441 tgccttgatc aggccctcgt tgtaggccac gcccgaggta ccccacatgt agttcacggc
     4501 gtgttcgttg cccgggtcga agacttcgag tcgcttggtg atgtccgcgt cgaggttctt
     4561 caggttgtcg agcttggact tgtcgagctt ctggtagatg ttcgacttga tctggcgcgc
     4621 caggtaggag gccgacggaa ccacgacgtc gtagccgctg cggccggcac ggagcttggt
     4681 ttccagcagc gcgttggagt ccatgacttc gtaattgacg tgcacgcccg tctcggtctc
     4741 gaattcttcg agcactttgg gctcgatgta gtccgaccag ttgtaaacgt tgacgacttt
     4801 ttcagcgtcg gtgtcgaata cggcagccgg tggagcggct tccgcctggg gcggaggcgc
     4861 ttcttctttc ttgccgcagg cgatcaggag gaaaagacag gaagccgaaa ccagagccga
     4921 aaggctgcga gtggacattt taagacccct aaatgttgtc gcgccgtgag acccacacgg
     4981 cgcgtcttta gacacgttat ataacagcga ccgactctat agccagccct cggcgcgcgc
     5041 ttgaatagcg gtttcatcga gagatcgcac tactttggac agtagttcgt ctatttcgct
     5101 ccgggagatg accaaaggcg gcgcgacgaa cagcgtatcg cgcgtcgcac gcatgacgag
     5161 gccgttgcgt gtgcaattgt ctcgcattcg cacgccaacc tcgccgcgcg gctcgaaaaa
     5221 ccgccgtgtg cgcttgtcgg gaaccagctc gatggctgcc agcaggccca cggagcgtgc
     5281 ttctcccacc aggggatgtg caagcagttc tttccaccgc tgcgctagat agggtgcggt
     5341 ttcctcccgg acccgttcca cgatgcgctc ccggcggaat atttccaggt tggcaatggc
     5401 caccgcacag gcggcgggat gcccggaata ggtgaaacca tgaaaaaact cgccgccctt
     5461 gtctatcaac acgttggcga cccggtcccc gaccatgagt ccgccgatcg gtagatagcc
     5521 ggaggacagt gccttggcca aggtcatgaa atccggacgc gttccataat gttcgcaacc
     5581 gaaccattgg cctgtacgac caaagccgca tatgacctcg tcggaggcga acaggatccc
     5641 gtacttgtcg acgatccgct gaatttcggg ccaataggtc tcgggcggaa tcaccaggcc
     5701 accggcgccc tgcaccggct cgccgatgaa cgccgcgatc cggtcagcgc cgacctccag
     5761 gattttcgcc tcgagttcgc gcgcgcattt cagcccgaac tcctcgggtg agaggtcgcc
     5821 gccctcgccg aaccagtacg gctgattgat gtgcacgata ccgggcaatg cgccgttcgg
     5881 ccccagttgg tcgtgcatgg gcttcatgcc gccgagcgat gctccggcaa cggtgctgcc
     5941 gtgataacca ttccagcgag acacaatcac atttcgctcg ggctgatcga gcagctgcca
     6001 gtaccggcgc accaggcgga tgagcgtgtc gttcgcttcg ctgcccgacc cggtatagaa
     6061 aacgtggttg aactgcggcg gcgtcacttc tttgaggatg cgtgcgagct cgatggcagg
     6121 ttgggtggcg ctctggaaaa agctgttgta gtacggcagc tccagcatct gttcataggc
     6181 tgcctccgac aactccttcc tgccgtagcc cagcgccacg caccacaggc ccgacatgcc
     6241 gtccaggatc ttgtcgcctt ccgatgtgta gatgtacaca ccctcggctc gcgtgatgat
     6301 gcgcgcgcct ttggcggcaa gctgcttgtg gtcggtgaac ggatgcaggt agtgagctga
     6361 gtccagccgt tgccactctt tcgtctccgg gctcgtgttc atacgttcaa cagcaggtgt
     6421 tcacgttccc aggagctgat cacctgcagg aagacctcgt attcggcttc cttgacgatg
     6481 gtgtacgccg atatgaacgg gtcgccgagg atgcggacca gcggctcgca ctcgcgcagc
     6541 tcgcggatcg cgtcatcgag cgagcgtggc agcgaaaacg gcagctcgtg agcgctgccc
     6601 gaaatcggct cggaaggctg gagcccttcg accatgccga gatagccgca ggccagcgag
     6661 gtggcgatgg cgatgtacgg attcgcatcg gcgccggcga ggcgattctc cacgcggcgc
     6721 gcctcggggt cggatgacgg cacacgcagg cccgcggtgc ggttgtcgta gccccactgc
     6781 acgttgatgg gcgccagctg gaagcgcgag atgcggcggt acgaattgac gttaggggct
     6841 aacagcgcca tggcggccgg gagatatttc tgcatgccgg cgatgtgcga aaagaacagc
     6901 ggcgtcggcg taccgtcgcc gttggagaag atgttcttgc gcgtcttgat gtcgacgatg
     6961 ctctggtgaa tgtgcatggc gctgcccggc tccttggcat gcggcttggc catgaacgtg
     7021 gcgtacatct tgtggcgcag cgcggcttcg cgcgccgtgc gcttgaagag aaatgcctgg
     7081 tcggcgaggt cgagcgcatt gccgtgcagg aggttgattt ccatctgggc ggcgccgtct
     7141 tcgtggatca gcgtgtcgat ctcgatgtcc tggtcctcgc agaactggta catgtcatcg
     7201 aacagcggat cgaactcgtt gaccgcggcg atgctgtagc tctggcggcc gggttcggcg
     7261 cggccggagc ggcccacggg cggcttgagt ggatagtcgg cgtcgatgtt cggttcgacc
     7321 aggtagaact cgagctcggg cgccaccacc ggctcccagc cgcggttggc gtacatctcg
     7381 aggatgtggc gcagcacgcc gcgcggcgcc atgccgacgc gccgcccgtc cgaatagaag
     7441 cagtcgtgaa tcacctgcgc cgtgggctcg gccgcccagg gaacccgccg cacggtcttc
     7501 ggatcggcct tgaggatgat gtcgatctcg gccgggctca tcgccttgtc ggtgtcgggc
     7561 gggtagtcgc cggtcacggt ctgcaggaaa atgctctccg gcaggcgcat gccctcgtct
     7621 tcggcgaact tctcggccgg catgatcttg ccgcgcgcga tgcccgccat gtccgggatg
     7681 atggcctcga cttcggaaat gccgtggtcg cgcaggaatt gtctgatttc gtttgccatg
     7741 gaagctacct aactactttt gtcctgttgc gcgccgcctt gccgaactcg gcgaacagcg
     7801 ctttcgaaaa cgggttgctc atcacctgcc actcgggatg ccactgcacc gcgagcgcga
     7861 acgccggcgc cttcgccacg cgaaaagcct cgaccagtcc atcggtggcg cgcgcctcga
     7921 ctgcgagacc gggcccgagc cggtccacgc cctgccagtg cagcgaattc acgcgcaccc
     7981 gctcctgtcc cgaaagccca cgcagcaccg aaccgggctc gagcaccaca tcgtgcgccg
     8041 gcccgtattg cacttcgaga ggctgctcct tgtcctcgcg atggtcatcc aggcccgaca
     8101 cttcgtgcac ccgctgatgc agcgtgccgc ccatcgcgac gttcatctcc tggaacccgc
     8161 ggcaaacacc cagaaccggc acaccgtcct ccaccgcgcg cgggatcagc gacagcgtgg
     8221 tcgcgtcgcg gtgcggatcg tgcagcgtgc ccggcacgct ctccgggccg gcataccgcg
     8281 ccggctccac gttcgacggg ctgccgggaa agaagatgcc atcgacgcgc gccaggatct
     8341 ccgagacgtc gagcggtgat tcgagcacgg gaatcagcag tggcagacaa tccgccgcct
     8401 gcacgatcgc cgcgatgtat ttctcgccaa ccgcatgaaa tggatggagg ccgatctggc
     8461 gtcgatcggc cggtatacct attaccggtc tataggcggg tctcttggag aaatgagttg
     8521 aactagccat acgctttcac gcgggccatt ggcccgggcg ctgtctatta tcttttacgg
     8581 aatcgcgcct gcgaccaggg cgagggacgt cattagacca cagcgcacgg ggtcccgggc
     8641 gagccgatgc ttggctctgc gcagccagcg aatgggccaa ttcagccgtt tggcccgttt
     8701 gccggcgtat ggcgcaccgg catatgctgc agcgcctggg caccgcccgg cgttgaaaat
     8761 aatagacacc gagacttaca tgactcgcgc cgccgaccca accctcgagc gcttcctggc
     8821 cgatcacccg aagattcgtt tcttcgatgc gttcgtgaac gacctcaata ccgtcgagcg
     8881 cggcaagaga atcgatcgcg ccggtattgc cacggtgttc gagcgcggca tgcccctgcc
     8941 cggctcgatg ttcgcgctcg atatcgaagg cggcactgtc gaagacaccg gcctcggctt
     9001 caacgacggc gatgccgatc gaccctgcat gccaattccg ggcacgctgg tgccggtacc
     9061 gtggcaaccg gatgtcgcgc aggtgcagct gcgcatgttc gatcacgacg gtacgcgctt
     9121 cttcggcgat ccccgctgga tactcgaaaa ccacctcgag ctgttcaagg cactcgacct
     9181 gacgccggtc gtcgccatcg aatacgagtt ctatctagtc gatgccgagc gttccgcggc
     9241 cggtttgccg cagcctccca ggggaccgct gaccggccgg agggaatacc gctcgcagat
     9301 caactcgatg gcggacctca atgagttctc accgctgctg tccgagatcg atcgcgtatg
     9361 ccacgagcag aacgtgccga ccacctcggc gctggctgaa tacggacccg gccaatacga
     9421 ggtgaatctc gcgcacggac cggacgcact tcgcgcctgc gacgaggcag tgcgattcaa
     9481 acgcatcgtc aagagcgtgg cgcgaaagca tggatgcgac gcgacctttc ttccgaagcc
     9541 gtatcgcgac atggccggta gcggactgca cattcacgtc agcctgctcg attcctctgg
     9601 ccgtaacgtt ttcgccgcag caagtgccga acagagcccg aagctgcgac acgcaatcgc
     9661 cggcacgctg gcatcgctcg gcgacggaat ggcaatctgc gcgccgggtc cgaactccta
     9721 tcggcgcttt cgtcccgagg cgtacgtccc gatgcacccg acctggtccg tcaacaatcg
     9781 cggttcagcg gtgcgggtgc ctgcttcgga cgcggccaat ctgcggctcg agcatcgcct
     9841 ggctggtgcg gatgcgaatc cctatctcgt cacggcatgg atcctggccg gcattcacca
     9901 cggactcaca cgtgggctcg agccgccgcc ggtgacgacc ggcaacgcct acgaacaatc
     9961 gggcgagccc ctgcccatag tctggagcga ggcgatcgag cgctttgcgg caagcgggtt
    10021 cgcgaaacaa tattttggtg aaaagttcac gcacctctat tcaacggtga agcgcgccga
    10081 agcccaggaa ttcaactccc acatcacgcc gctcgaaatc gcgcgttatc tgggcccgct
    10141 ataggccgga cccgatgact ggcgccgaat cgaactacta cttcgcgacg tctccgccgc
    10201 caccgccgca cgtgcccttg cgcggcgaga tacgcgcgga cgcgtgcatc gtgggcggcg
    10261 gaatctccgg cctttccgcg gcgctgcatc tcatcgaacg cggcttcagc gtggcgctgc
    10321 tcgaggcgaa gcaccttgga tttggcggct ccgggcgcag cggcggccag tccatcttcg
    10381 gttatgccag tagccaggaa aaactcgaaa agctcgtggg cgagaccgac gcgcgattca
    10441 tgtgggacat cgcgctcgag ggcatgaaac tgcagcgcga actgattgcg cgtcattcca
    10501 ttggctgcga ctacgtggca ggccacatgc acgtcggcgt gaagtcgcgt catgacgccg
    10561 agctgcgcgc ggaggtcgag tcgctgcact cgaagtacgg ctatcgcagc gtacgcctgg
    10621 tcggccacga agagctgcgc accatcgtcc agagcgaccg atataccacc ggcatctacg
    10681 acgccaacag cggacacctg aatccttaca agtacacgct cgggctggga caggccgcgg
    10741 cgcgggccgg tgttcgcatc ttcgaaaact cccgggtgac gaagctcgag ctggccgccc
    10801 gtgccgacgc cgacaacctg gtgcacaccg aatccggcac cgttcgcgca cgctacctgc
    10861 tgatcgccgg caacgccttg ttaggccggc tggtgcccgc gctgtcgcgc aagctcatgg
    10921 cggtgggcac gtacatcgcg gccaccgaag tgctgggcga ggaacgcgcg cgcgcgctga
    10981 tcaccaacaa cgcggcggtc gcggacatca actgggtgct cgactatttt cgcctttctg
    11041 cagactaccg cttgttgttc ggcggccgcg tgagctactc gggggtcgat cctttcgatt
    11101 ccgcacgcgt cctccgagcg cgaatctcgc gcgttttccc gaagctgaat ccgcgcatcg
    11161 actacgcgtg gggcggctac gtcgacatca cgccgaaccg cgcgccacat ttcgggcgac
    11221 tcgcgcccaa tgtctatttt ctgcaggggt tctccggcca cggcatggtg ctgtccggca
    11281 tcgcggggaa actggtggcc gaagccatgg cgggtgtcgc tgcccgcttc gacgtgttcg
    11341 cgaagattcc gcatcgcgat tttccgggcg gcacctactt ccgtcgcccg gcactggtgc
    11401 tggcgatgct ctggttccgg ctcaaggatc tgctgtgagc ggattcgatg tcggggtgtg
    11461 gttgtcggcg ctgccggtgc tgctgatcgc cgccacggtc acctggctcc tgtcgctgtc
    11521 gatgcgcaac gtctccatcg tcgattcgct gtggagcctg atgttgttcg ccgccggggt
    11581 catctacgcg ctgggcgccg atccgcgtgc gccgcggctg gccttcgtgt tgtggatgct
    11641 ggcgatctgg gcggcgcgac tgtcgctcta catcaccgtg cgaaacatgg gaaagggcga
    11701 ggaccggcgc taccgggcca tccgcgagcg caacgagccg cacttcgcct tcaaaagcct
    11761 gtacctggtg ttctggctgc aggcactgct ggcctggatc atttcgctgc cgctgctggg
    11821 ggcgttttcg agcattgcgc cgatcggcat cctcgattac ctggggctga tcctgtggct
    11881 gaccggcttc gtgttcgaag ccctgggcga ctggcaactc gcccggttca agagggaccc
    11941 ggtcaatgcc gatgccgtca tgacctccgg cctgtggcgc ttcacgcggc atcctaacta
    12001 cttcggcgag ttctgcatct ggtggggatt cgccgccgtg gcggtttcgg ccggtggctg
    12061 gtggtcgctt cccggacctg cgctgttgac cgtcctgctg ctgcgcgttt ccggggtcag
    12121 cctgctggaa aaggacatcg gcaatcgtcg gccgcgttat gccgactacg tcttgaaaac
    12181 gaacgcgttt ttccccggtc ctacacgcaa atagcctaaa gggcgccccg taatgggccg
    12241 ataacccccg gagacaaccg gagtcggcac caggattagg caggcaaacc cgtgatcgaa
    12301 gtggattcag cgcaatgcga ggctcgtgcc cgtgaagtgc gtcacgatgt cgtggcgacc
    12361 ttcacgctgg ccacgcatcg catttttcgc tacgtcttcg cggtgcagtg ggcgatcgcg
    12421 gtggcgatgg catggcaatc gacgctgccg ggcgaaccgc gcgtcgtgtt cacgatgatc
    12481 gtgggcgcca tgctgtcggt tccggccgtg ctgttcgcgc gcgcggctcc gcaagcggcg
    12541 tgggttcgcc attacacggc ggccagtcag atcggctggt ccattctttt catgtggctg
    12601 ctcgaaggac aaccggaatc gcagctccat gtattcatag cgctggcgtt catctcgttc
    12661 tatcgcgact ggcgcgcgct catgacggcg acattcatcg ccctcgcctt tccgatcgtg
    12721 cgcctcgcgc tgctgcccga gtcctacacg atcggcgccg cggcatggtg gcgcgttttc
    12781 gaccacgcca cgtgggtgtt ttcggatgca gtgatcatgc tgctcgcggt gttgcagagc
    12841 ctgaagacta ttcagaaatt cgcgctgcac tcggccacgc tcacgctgac caatgaaacc
    12901 atcggcaaac acgtggagga acgcacgcgc gagcttgcgc gcagccgcga gcaataccgc
    12961 ctcattgccg aaaccacgcg tgccataccg ttcgaactcg acctggcgaa cggccgcttc
    13021 acctacatcg gaccgcaggc gcaaaagatg ctcgggtttc cggagtccca atggaaggaa
    13081 ccggaatttc tcgatgtgct gttaccccgc tcgcgcgagt cgaacgtacg ccagcagatc
    13141 gacgagtgca cgccgggtac gttcgagaca ctgtgcagcg tagtcaccgc cgacaatcgc
    13201 gtcgtggagt tgcgctggac tgtgtcctgc gagctggtca acggcatgcg cttcctgcgt
    13261 gggctgatga tcgatgtcac cgaggcgcgc cgcatggtcc gcgaactcgc gcagggccag
    13321 aagctcgagt ccgtgggccg catcgccgcc ggcgtggctc acgagatcaa cacctcggtg
    13381 caattcatct ccgacagcgt tcgctacgtg cgccacgcgc tgcgcgacgt gcctcacgcg
    13441 ctcgccgact atcgcgcgct cgccgccagc gccttgtcgg gcggagacgt gaccgcggcc
    13501 gcgaaaaaag ccgccgatac cgacgaagcc gcggacgtgg actacttcct caagaacgcg
    13561 ccggatgcgc tcgatcgcgc gctcgaaggc attggccgca tgggttcggt cgtgcgttcg
    13621 atgaccgagt tcgcgcatcc cgactcgcgc accaaggccg atatcgatct caaccgcacg
    13681 atcaggacga cgctgaacat ggcgcgcaac gaatacaaaa gcgtcgccga gctcgaaacc
    13741 gacttcggcg acatccccgc ggtgcattgc cacgccggcg acatcaacca ggtcgtgctg
    13801 aacctggtgc tcaacgccgc acatgcgttg ggcgatcgag cggcgggttc gatgtccaag
    13861 ggccgcatag ccgtgcggac gcgcgcgatc ggcggttatg tcgagatttc gatcagggac
    13921 accggcgacg gtatccccga agatgtgcgc gggcgtatct tcgagccatt cgtgaccacg
    13981 aaggaagtcg ggcgcggcgc cgggcaaggc ctcgcgctgt ctcgcggcat cgtggtcgac
    14041 aaactcaagg gttctcttca cttcgaaact gaaactggaa aaggtacgac cttcttcatc
    14101 cggctccccg tgtcggatac agcgctcgcc accgattcca acaacaagca ggctgccgca
    14161 tgacttcgct acacgtgtta tttgtcgacg acgaacaggc cattctcgat ggcttgcgca
    14221 attccctgcg gaaggaacgc aagcgctggg acatgacctt cgcgctcggc ggccagcagg
    14281 cgctcgagga gatgaaaaag actccggccg acgtcgtcat caccgacatg cgcatgcccg
    14341 gcatggacgg cgctgaactg ttgcgccaca tccagcgcga ctttccggcc gcggcgcgta
    14401 tcgtgctgtc gggccaggcg gaacgcgaat ccatcatgcg cgcgctgccc gtcacccatc
    14461 agttcctgca caagccctgt gatggcgaaa cgctgcgcgc ggtcatcgaa cagacccatg
    14521 cgctgcacaa gctcatgcag aaccccgcct tgaccgcgct cgtgggcaag ctggaacgca
    14581 ttcccgcggt gccctcgact tacaccgagc tgtcgcgcat cgcggccgat ccgaaagcgg
    14641 acagcgcgat gttcgccaag gtcatcgaaa tggacgccgc ggtctgcgcc aaggtgctgc
    14701 agctggtgaa ttcagcctac ttcggcctcg gccagaagat cgtcagcatc cggcccgccg
    14761 tgacctatct cggtgtcgag atcatcaagt cgctggtgct gagttcgacc tcgttctctg
    14821 acaaggccat cagcgaagtc aagggcttct cgcccgatcg cctgcagcaa cactcgatgc
    14881 tcacggcgat tctcgccaag aagatcgtca gcaacccggc gctggccgat gccgcgttca
    14941 ccgccggcct gctgcacgac atcggcgcgc tggtgctgct gcacgccgcg ccgcccgact
    15001 acgtgcgggc gctcgagcgc aagaaggagc tcaacggcga cagtgcggcc gccgaacgcg
    15061 agatcttcgg cgtcacacat gccgaagtcg gagcctatct actcggcctg tggggcatcc
    15121 cgtttcccat cgtcgaggcg gtggcgtttc atcaccgtcc caacgaagtg gcgcctgagt
    15181 cgcgcccgct ggtcgccgcg gtccacatcg ccagcggcct cgtcgaagag atgaccgaga
    15241 gcaatggcgc gcaggtcctg ggcggcacca gccccgacag ctcggcgcgc atcgacgtgt
    15301 ccttcctgcg cgaagccggg ctcgaagagc tgttcaagaa gagccggacc gaggcggaga
    15361 agctcgtcgg cggagtagcc gaacgctcct gagctggcta gcatcggcgc ttcccgaacg
    15421 aggagagcgt cgatgcggat tgcacggatt acggcggtcg tactgtcact gaccgcaggc
    15481 ctggcgggcg ccctgctcgt gtcaaccacc gatgcggccg gcacgcccgg ggcaccgacg
    15541 agcgctgccg cagcgtctgc gttcctcaag tccatgcccg cggatctgcg cgcatcggcc
    15601 agctttccgc tcgaagcgcc cgagcgcctg gtgtggaact tcgttccaat ggaccgcgtt
    15661 ggcgtgtcgc tgctgaaact cgacgacgcc caaagcgagt tgctgggacc ccttctcgcc
    15721 accgcgttga gtccggaagg gctgctcgcg gcgcggggcg tcatgaaaca tgaaaacatc
    15781 ctgcgccgcg tggaaaccga ggccggcgtg gcgaatgcat cgcgccgcga ccccgggctc
    15841 tactacaccg ccattttcgg caagccggcg ccggcggcgg cctggggctg gcgcttcgag
    15901 ggacaccacc tctcgatcaa cgtcaccgag cttcccggcg agtcgcccgt tgtcgcgccg
    15961 atgttcctgg gtgcgaaccc tgcgcgcgtg ctcgcgggac ccaatgccgg ctttcgattg
    16021 cttgccgcgg aggaagatct cggccgtgag ctgatcaaga tgttgcccgc ctcgcgccgt
    16081 gagagcgcga cgatccgcga taccgccttc tccgagatcc tcacgggcaa tgatcccaag
    16141 gtgcagaagc tcgagctcga aggtctcgcc gcggccgaaa tgacagcgga agaaaaggcg
    16201 cagttgcgcc gcctcatcga gctgtacacc gggcgcatga cgccggcttc cgccagggac
    16261 gcgttgtcgc gcctcgatcg ggccggtttc gacaaagtgc gcttcgcctg ggcaggcggc
    16321 atcgagcccg gccaaccgca ctactatcgc gtgcatggcc ccaccctgct catcgaatac
    16381 gacgacacgc agaacaacgc caatcacatt cacaccgtct accgcgacct ggagcgcgat
    16441 ttcggcggcg atgcattgcg ggcgcactat cgaggagcac tatcgaggcc atagacaatt
    16501 gccgatagcg gcccgcgcga cgcatggcta gactcaccac atcgcgtgag gagtcaacac
    16561 atgggcaaga agaacaccac atccgccgcc acgatggccg caccgctgat cgacatcggc
    16621 atcaagggca aggatcgcga gaagatcgtg caagggctgt cgcgcgtgct cgccgacacc
    16681 tacacgctct atctgaagac acacaacttc cactggaacg tggaaggccc gatgttcaac
    16741 acgctccacc tcatgttcat ggaccagtac acggaactgt ggaacgcgct ggatgccatc
    16801 gcggagcgcg ttcgcccgct ggggtatccg gcgccggcca ccggcaagga attttcgaag
    16861 ctcacatcga tcgaagaaac cgaaggtgtg cccgacgcgc tcgacatggt gcggttgtta
    16921 gtggctggcc acgaggccgt gacgcgcacg gcgcgctccg catttccggc cgccgagaag
    16981 gccggcgatg aatcgacctg cgacctgttg acgcagcgcc tgcagattca tgaaaagacc
    17041 gcgtggatgc tgcgcagcct gctgaagtga gagttccaat atcgcggatt gccgcccttc
    17101 tggcagcggc ggcaatcctg ttcgcgggag caaccgccgg cgccgcgaaa ctcaaggtgg
    17161 cactcacctt cgacgacctc ccgctcaatg gcacgctgcc cacgggcaag aagcaatccg
    17221 atttcgcgcg cgacaccatc aaggtgttga agaagcaccg cattccgccc tcctacggct
    17281 tcatcaacgc gaacaagctc gaacgtaacc cggatggcgc gcaggcattg cagatctggg
    17341 ccaaaggcgg ccatccgctc gccaaccaca cctacacgca cctcgacctc acgaagaaca
    17401 gcgtcgaaga cttccagcgg gaaatcctgc gcaacgaacc cgcgctcgaa ctgctgatgc
    17461 cctcttcttc tcaagatagc ggtgggaaac acgggcgctg gtcatggttc cgctatccgt
    17521 acctgcacga aggcgacacg ctcgagaagc ggcgagcggt gcgcgccttc ctcgccagca
    17581 gtggctaccg catagcgcag accaccatcg acttcgagga ctatctctgg aacagcgcac
    17641 acgcgcgttg ctggatgaag aaagacgcgg aaggcctcga ctggctgcgc gagagctatc
    17701 gcaccgccgc ccgcgagttc atcaggctgg acatcgccaa ttcccgcgcc gccttcggcc
    17761 gcgacatcca ccacgtgatg ctcctgcatc tcgggtcgtt cagctcgcac atcctgccgg
    17821 accttttcga tatcctcgat gaagaggggt tcgaaatcgt cacgctcgag gaagcgcaga
    17881 aagatccggc gtacgactac gatccggatg tcgccgaccc gcgcggcggc actctcgtgg
    17941 agctcgcgat gaaggcgaag aacattccct ggcccgccaa cgcgccaaaa aagccgcgcg
    18001 atcgcctggc cgccatctgt aactagttag cgcatttaca cgccatccgg ccgcacaggg
    18061 gctccggacg ccgccgaatc gtagatcgcc cgcaccagcg ccacggcctt gcgcgcctcg
    18121 ctgccctcga tgaagaacgg ccgctgctcg cggataccat cgatgaaatc ctggatctgc
    18181 cgccggtggc cttcgaaatt gatggccatg gggttggcgg cgcccgatcc cttggcgctg
    18241 gtctcgcgcg ccctgcgaat cgcttcgtct tccggcgccg cgacgcgaaa ttcccagctc
    18301 gcgatgtcgt cgtcttccat gaccgccgaa ccgttctcgc cgcagatttc gattcggcgg
    18361 gagaatccgg gccatagcgc ggtgctggct tcgatggtgc cgaacgcacc gctgccgaaa
    18421 cgcagcgcgg cgacggaggt gtcttcggat tcgatggcgt gaacacgctg cgtggtccag
    18481 gcaaacacct ccaccggcat gccggcgaac cactgcagca ggtcgacacc gtgaatcgcc
    18541 tggttgatga ctgcgccgcc gccgtcctgc gaaatcctgc ccttccagcc cgtgtagtac
    18601 ccgggggtgc gattccactt cacgtagcaa ctggcgagca ccatgcggcc aaaacgcccg
    18661 gcgtcgaccg ccgccttgag cgaacgggcg gcatcaccga agcgcgcctg gaaaatggag
    18721 ccgacgcgca cacctgcctt cgcggcttcg tgcagtatgt gatccgtacc ctcgacggtg
    18781 gagtcgagcg gtttctcgat catcaggtgt ttgccggcgc ggatcgccgc gagcgcgggc
    18841 acgaggtgca gcgccgacgg cgtgctgatg cacacggcat cgagattcgg ctgcgcgagc
    18901 agctgagcca catcatccga tgcgaaaccc atgccgtgct ccgctgcgaa cttttcggcg
    18961 gtggcgcgcg tgcggctgac gacgcccacc aggtgtccgc cttgggacgc tgccacggcg
    19021 cgcgcgtgga aattcgcgat ggcgcccgca ccgatgaaac cgaaacccag tgtttgactc
    19081 acttcgtttc cttgcggagc atgtcagtgc gcgagcgcga tcgggtcgtt gcggtacacg
    19141 gcatcgcgga agatcttgtg cagggacccg tcgaacggcc gaacctgcgt gaagaacacc
    19201 gcggtgatgt cgttcttcgg atccacccag aacaacgtgc tggctgcgcc gtcccagaaa
    19261 aactcgccga ccgcaccgga aggttcctgc gcatccttcg gcggcgcgat gcgcacggcg
    19321 aaatcgatgc cgaagcccac ctgacccttg ttgggcagcc agcccttgtc ggtgacttcc
    19381 ttcggcatgg cgtcggtggc catgagcctg acggtctcgg gcttgaggac tcgcgcgcga
    19441 ccgagcttcc cgctacctgc caacatgcgc gcgaacttca tgtagtcgtc gatcgtggag
    19501 acgaggccgt agctgccggg cttctgcggc cacgcggcgc tgttgaaccg gtaggcctcc
    19561 tcgtccgtct ggcgagtgag cgtgccgcca tcgcgtgtgt agatcgccgc gagctgcggg
    19621 cgatccgcgt cggtgggcag gatggtgtac cgcgtggagg tcatgccgag cggacgcagg
    19681 atgtgcagca tgagaaattc gtcgaatggc accccggaga ttttttcgac gagatatgcc
    19741 tgcacgtcga cgacgtcgct gtatttccac tgcgtgccgg gttgatagct caaaggaacc
    19801 ttggcgagcc gggcggacac ttcgggcagt gcgttgtcga ggttgcgggg atcgatctgc
    19861 cggtagatag ccgtgaccgc ctcgggggag ccatcgccgt ggaaccccgc cgtatgacgc
    19921 aggatgtcgc gcacggtgat gggccgcttc ggcgcttcgt atttcggctg gccgttggcg
    19981 tcgaggcccg cgtagacctt cacgtcggca aactccggcg cgtaaacgga gagcggcgcg
    20041 tcgaggtcga acttgccgcg ctcgtacagc cgcatgagcg ccacaccggt caccggcttg
    20101 gtcatggaga aaatctgcac gatggtgtcg cgcgtcatcg gcttgttgtt ttcgcgatca
    20161 gagaggccga aggcgccgaa gtaggcctcc cgcccgcgtt cgtacaccag cgccgaaact
    20221 ccgacggcct tcttgccgtc gatgatactt ttcagcgcgc tgtcgatggc cgccgggtcg
    20281 acgagcggtg gtggcggagg gacgggaacg accggcgtcg gcggcgatgc tggagcgggc
    20341 ttcggcgcat ccgctgcgac ggccgaaccc aacacgcccg tgataatcac cgaagcagcc
    20401 agtttgaaaa acttcatcga tcccctcctc gtttaggcgt ggaacacccg acgcgcgcct
    20461 tcgacagaac cacatcgtcg atccacagat tttgatcgtt ggccgagtcc gcgtatcgct
    20521 cgaagccgat gtaaaagctg tcgaatttcg gcggcgcgcg ccactcacct tgcagatcgt
    20581 tgccgcggca ggccttggca gccgtggccc ttccggcaac gtgcacgacc ttgcgtccgt
    20641 cgagccagaa ctgcatttca ttcgaagcga cttccatatg ccattcgacg caggtccagc
    20701 gcttcacggg cagggtccgg tccgaattct gcttgcaatc ggtggtgacc ggcggtgtgg
    20761 tttcgaaatt ggccatgagc tgcgtaccac ccttgccttc cagccccagc cggtagatgg
    20821 cgttgtagcg gtcgtcggcg gagcggccct ctccctgcaa cagggtccag tgcacggccg
    20881 tcccacctgg cacggcttca ggcgcttcgt cgagaaacac catggcgcgg ccgaacatgc
    20941 cggacatggc cgcaggcacc ggagtcccga ggtgaatggc cacgtagcca cgccggtagt
    21001 ttgcaccgcg cggagtgaag atgtgcaacg cctggtggcc gctgtaggcg tgatcggcat
    21061 caaccgagat gacggcaccg gacttgaagg tggcttcgct ccagggtgcg ccagggaatt
    21121 cggcggttgc gtgggattcg aaatcgtcgg tgaagaggat tggatgatca gcggctggag
    21181 atgaactcgg gcaaaggacg aagagcaagg tcgcgaccgc gggtgcagcc gccatttttc
    21241 gaagacgaaa aaatgggcgt agtccgctca cgggtgagcc acggcgatga ttcggtaaca
    21301 gacctcgagc gtgggcatgg gaacgcgaaa ttcgcgggct ttttccagcg cgtagcccag
    21361 ggtctcctcg tactcgagcc ggctgccacg caacacatcc tgttgtgccg acatgcggtg
    21421 ctccggcgaa gtctcgaaaa acttctggcc gatgccacgc acgatttcaa ttgcctgggc
    21481 gtcggagccg tgcacgatgc tgggtacggg cagcggcggc atgtcggtga gctcgatctt
    21541 catggccgcg gccagcgctg cggcctcgcg cacgatacgc acgatgacca tggcgctgcg
    21601 ttcgtccatg aggaacttcc aggtgatctg ccgcgtgagc accgcgagcg gaaactgcgc
    21661 gatccatccg gcgaacttcg accactcgcg cgtgcggatg ttggatgccg ctgcgcagcg
    21721 cactccggcg gagtcgatga gcgcggccag cgcctccgca cgcgggctcg agccgccctt
    21781 ctcttcaccg aggtgcaggc agacgttgcg tgtgaacttc acttcgccgt tggcgaggag
    21841 ttccccgctg aaatccgcca tggcgcctag cacgcgcgaa aacccgaata accgcgccag
    21901 cagttcgttc ttgagtacgc cattctgcac cgagaaggca ttctcgagcc gcaggtgctt
    21961 gagagtgtcc aggctgttcg cggaatcgat ggccttggtg gcgatgatca gcgtgtcggt
    22021 gtccaccagc ttcgaagggt tgtccaccac gatgggacgc gccttgatgt ccgagaggcc
    22081 gttgagcacc agcccctggc gttgcaggac gcgcgcgcgt tcgccgcgcg cgatcatgac
    22141 gacgtcgtgt ccggcgcgcg acaggtgcga gccgagaatg gtgcctagcg cgccggcgcc
    22201 gagaatcgca aaccgcatga gcttccgtgc tccctatttc ttccggtact tctcgaacca
    22261 ggccaggatg ttatccacct tggcgatgag attgctggga cggcccgtca tgccgtgcga
    22321 agcttcggga atgcgcacca tcagggtatc gaccttgcgc agcttcagcg cctggtagta
    22381 ctgctccgac tcggagatcg gcgtgcggta gtcagcctca ccggtgagca gcatggtggg
    22441 cgtctttacg ttccccacca gcgacaacgg cgaacgcttc cagtattgct ccggctgttc
    22501 ccagggctgc gcgccgaacc agtacttgta gaagaaattg ttggcgtccg aggtcaggac
    22561 gaagctcgac cagttgatca ccggcttggc gaccacggcc gcacggaagc gatccgtctt
    22621 gccgacgatc cacgcggtga gcactccacc tccggaaccg ccggtgacaa aaagattgct
    22681 ggtgtcgacg ttcccctcgg cgatcaacgc atcgacgccc gacatgaggt cgtcgtagtc
    22741 gttgccggga taggcgtgat ggatgagatt gccgaattcc tcgccgtagc tcgtgctgcc
    22801 gcgcgggttg gtgtacagca cgatgtatcc cgcggccgcg tacagctgca gctcggtggt
    22861 gtagttggga ccgtaagcgg tgaacggacc gccgtggatc tcgagcacca gcgggtattt
    22921 cttcgccggg ttgaagtcgg gcggcgtgat cacccagccc tggattgccc gctgatcatg
    22981 cgaactcttc cagttgagct cgcgtacctg gcccagcgcc ctggcaccga agagatcgtc
    23041 gttgagcgcg gtgagcagtt tcgtggcacc acctgcagtg gccgtcgcga catcggcagg
    23101 ccgcgagggc gcattgtgcg tgaatgccac gcgcccattg cgcgcgacgc tgtacatgcc
    23161 ggaggtgtac ggacggccga ggtccgtgcc acccaggcct tcggcgatat tgcgcacctt
    23221 gccgtccagc gacaccacgc caagcttttt cactccgcgc tcgtcatacg agaagtacac
    23281 gctgcggcta tcggcggccc agcgcggatt ctcgatgtcc tcgtcgaact gtgccgtcac
    23341 gacccgcgag ttcttcccgt cggtatccat cacatataga tgagtaacct ggtatccctg
    23401 cagccggtcg tcgaagccca ggtaggcgat tcgcttgcca tcgggcgaca ccacggggga
    23461 gttgtcggga ccgtcgcgcg tggtgagttg ggtgagtttc tgcgtcgcga tgtcgacgtc
    23521 gaatacttcg ctttcctgcg gatcgcgctc ccagtccggg ccacgattgg ccgaaaacac
    23581 gatgcgcttg ctgtcgggag tgaaggacag cggtccacca tcgttgaact cgccctcggt
    23641 gagctggcgc ggcgttccgc cttccgcgga aacgatgaac acgtgctggt agccggcctc
    23701 gagatagccc tcgccatcgg cgcgatagat gaccgaatcg atgactttca cgggcggcgc
    23761 ccactgcgcg ccctcgggtt tttccggcgg cgtcgcgagc ggtggcttct tcaccggcac
    23821 gagttgcgtg aacgcgatcg acttgccgtc cggcgaccag gcgatggctt ccggtgcttc
    23881 gacgagattg gtcagcaatg ccgtctgtcc ggaatccatc cagcgcacgt agagctgcgg
    23941 actgccctcg gccgcggaca cgtacgcgag gcgcgtgcca tctggcgacc agcgtggcga
    24001 gctgaaattc ctggactcgg acctgagggg acggttgtta gcgcccgtgg cgtcgacgac
    24061 ccacagattg cggcgcgcgc ggtcggtcat gatgtcgtga ctgacacgca cgtagacgat
    24121 gcggttgccg tcgggagaga tctgcacgtc gtcggcgtat tcgagctgga acacgtcgat
    24181 ggaatgcagg cgccgcgact cgggcagatc ggccgcgcca gcgcaaacag caaacaacaa
    24241 tgccgtggcg gacacggcga gcgaacggaa tttcatgggg aatcctgcga aagaaagtga
    24301 gcgtacttta accgagtgag cacaccgcgt aacctcgacc agaggatgtt gtccgggagg
    24361 gaatcatgcg tacgatctgc tggcacgtgt ttatctcgat gggggcggca atgggtgcta
    24421 cttccgcgca ggcggcggac gccgcaccag accgtgaggt ctcgctcacc atctacaaca
    24481 agaacatcgc tctcatcgag catgtgcgcc ccatcagcgc tccggcaggc cggcaacgca
    24541 tcgagtttac gggcgtctcg gcgcaaatcg ttccagagac cgtgagcttc atcgcgccca
    24601 acgtggtgct ggtcgagcag aacttcgact acgacctgct gacgcccgcc aagctcatgg
    24661 aaaaggcggt tggcagccag gttcgcgtgg tgcgcgtgaa tccggccacc ggagcggaaa
    24721 ccaccgaaac cgcggaggtt ctctccacgg tgggcggcgt ggtgttgcgc atcggaaccc
    24781 gcatcgaggt gctgcgcgaa gacaatctgc ccacgcgtgt catcttcgac aaggtgccgg
    24841 aaaacctgcg cgcgcagccg acgctatcgg tgctggtcaa cgcggcgaaa ccggtcaacg
    24901 aagtcgcgcg cctcacctac ctgtcgcgcg gcttcacctg gtccgcggaa tatgtggccc
    24961 tgttcgatga gacccgcggc accacctcga tgcagggctg gatcacgctg cgaaacacct
    25021 cgggcacgag cttcaccaat gcgcgcacac agctgattgc cggtgacgtg aatctggtcg
    25081 attcagagga gcagtggtgg acgcggtggc aacaacggcg cagtaccagt caacgatccg
    25141 cgggcaccga aagtaccggc cgccagcagc ttggcgacta ctacgtttac ccgatcaggc
    25201 aactcaccac ggtcgcgaac aaccagacca agcaggtgag tttcatcgac gccgaaaacg
    25261 tcaaggccag caagggttat gagttgagtt tctatggctt ccagtcgaac accgagccgc
    25321 agtccgcgga aatccgcatg cgaattgaaa actcgaaagc cgccggcatg ggtgaacagc
    25381 tgccgagcgg cgtggtgcgc atctacatgc gcgacgcccg cggccagccg cagttcgtcg
    25441 gcgaagacct catcagtcac accagcgcgg cttccgagct ggcgctcaag atcggcgatg
    25501 cgttcgatgt caccgtgcag cccactctgc tggagacccg gcgaatcacc aagcgcaagt
    25561 ctgaattcga catgagttac ctcgtgcgca gcgcccgcgg cacacccagt gtcgtgacgc
    25621 tgcgccagtt tggcctgtgg cgccagaacg aaatgctcaa ggaatcgatc gtgggccggc
    25681 gcaccgacgc caacagcttc gcctgggacg ttccggtgcc ggccaacggc gaaacgaccc
    25741 tgacattcac cgtcgccacc agctggtaac acgcgtgcga tcgacgacgt tcatcgtgat
    25801 ggcgctgctg gccggcgcgc attccgcgcg cgccgatacg atggaggtcg tatccccgcg
    25861 ggccgacgcc gtgtccgtga ctatctaccg agacctgttc gcgctggtca ccgaaacccg
    25921 cacggtggat ctgcccgagg ggccggtgac gctgtcgttc gatggcgtgg tcgaaacgct
    25981 gctgcccgag tcggcagtgg tcaccgacct cggccgtgcg ctcgaggagc gcaacttcga
    26041 ctacgacccc ctctctccga acagcctgct ggccagatcc atcggcaagc acgtgacgat
    26101 cacgcgcacg ctcccgggcg caggcagggt cgtgcagacc cgcgcggtca tcgcagccgc
    26161 gaatgccggt ggcatcacgc tgcgcaccga atcgggcaac gaggcattgc actgctcggg
    26221 aattcccgaa aggctcacgt tcgatgaaat ccccgctgac ctgaatgcgc ggcccacact
    26281 gtcgatccgg ctcgccgcag gcccggccgg caaacgcacg attcgcctga gctacctcgc
    26341 ccatggcttt gcgtggaaat ccgactatgt ggcaaggctc aacgccaccg gcaatcgcat
    26401 gagcctcacc gggtggatca cgctgcagaa ttacacccag gcaaatctgc gcggtgcgca
    26461 ggtgcaggtg gtcgccggaa aattgcacct ggtcgacaag caggaaggcg gttcgagcct
    26521 caccggtgac agcgaagact tcgacgacga attccggctg aaccaggcgc gtgaaagtgc
    26581 gctgcaggac ctgcagcgcg agctcgagcc aaaggacagc acgttgcagc tgttcagcgg
    26641 ctgtcacgca cttccggttc cagacactcg caacgacaag ctgtccgatt ttgcgtcggc
    26701 ggaaatcgtc gtcactggtt ttcgcgggac catgcggatg agcatgaaca tgaagcgcga
    26761 tgtgatgacc gcggtgcgtg aagaactcgg cgactaccag ctctaccggc tgccctgggc
    26821 caccgacctg aacgcgcggc agaccaaaca agccgtgttc ctcgtcaaac ctcgcgtgaa
    26881 ggtcgaccgc ttctacagct ttgccttcga cgccgggaac atcgaggcgc tggaaacgta
    26941 tacacccgag tcgatgttcg cgtttgacaa tcgaaagtcc tccgggctgg gtgagccgct
    27001 gccgcaaggc ctggtgcgca tgtttgaaac cgccgggagt ggcgacgttt tcctcggcga
    27061 atcacgcatg gacgacaacc ccgtgaacgt cccggtggaa ctcgcactgg cacaggcgat
    27121 cgacctcgca ctgacgatcg acgtcgatcc gggcgatcca gaggaattgc gtgatgccaa
    27181 tctcgacgaa cgcgccgata tcttcgcgcg gttgtgcaca tacgcgtaat ggcggatctc
    27241 gagagtcacc ggcacgccct tggcgttgag tgcgtgcaac tcgacatcgg tgcgt
//
LOCUS       DASQJS010000119        18718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_63952, whole genome shotgun sequence.
ACCESSION   DASQJS010000119 DASQJS010000000
VERSION     DASQJS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18718
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63952"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VEW08_04845"
     CDS             complement(<1..873)
                     /locus_tag="VEW08_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ40094.1"
                     /translation="MPIDKSSLAPAFILPLAALILAGCARNEAAETPAAPPPVQAAMV
                     VSKSITEFDEFTGRFEAVERVEVRPRVSGYVMATRFAQGHEVKKGDILYVIDPRPYQA
                     TLKHAQAELTRARTQAALAESERVRAQKLIEKRAISQEEFDARISGNEQAAANLQASE
                     AAVESAALDLSFTEVRAPISGLVGRAEITAGNLVAAGQTLLTTVVSIDPIYVSFDGDE
                     QVYLKYVDMALRGERGSSRDTQNPVCVGSANEQGHPHEGVMVFLDNELDPNTGTIHAR
                     GLFKNADRRFTPGMF"
     gene            1082..1609
                     /locus_tag="VEW08_04850"
     CDS             1082..1609
                     /locus_tag="VEW08_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYJ40095.1"
                     /translation="MPTHMEIVLQAAAFAAEKHRNQRRKDAEASPYINHPIQLAYILV
                     QADVEDPIVLAAALLHDTIEDTNTTHDEIEIVFGHEIANIVVECSDDKTLDKLERKQA
                     QVDHAAHISNRAKLVKLADKIANVSDIDGAPPAGWSLERKREYFDWAKRVVDKMRGVH
                     PKLEARFDAEYAKRP"
     gene            1606..2211
                     /locus_tag="VEW08_04855"
     CDS             1606..2211
                     /locus_tag="VEW08_04855"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYJ40096.1"
                     /translation="MTSIVRWLLLANVAVFGLQQLSRDELLIHFALWPLGAGFEPWQL
                     VTSAFLHDNLPHIALNMYALWMFGSMVERVLGPGRFAWVYLASVLAGSLVQLLVVTMA
                     LDRGITPTVGASGGVFGILLAFAMLFPHSRLYIIPVPVPLKAWVVVAGYAAIELASGV
                     MGTSQGVAHFAHLGGMLGAAVVMLAIGFRKSVQPPGDLRGP"
     gene            2218..3426
                     /locus_tag="VEW08_04860"
     CDS             2218..3426
                     /locus_tag="VEW08_04860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ40097.1"
                     /translation="MNAGHPHDRQWLYAAVLARSVATGMMGVLLGIYLASLALDANAI
                     GVVIGLGLAGAAFGTLLVTYFSDRFGRRRTLFVITALTVCGAVMLAFSMSPLTLGLAA
                     FLGMVNGMGRDRGAALVVEQSVLPATVTDSQRTMAFAKYNVLQDVGHAAGSLLAAAPA
                     LFQRFTLLDAAHSLKASIFVYVALSLLPFLAYLRLSDRVEVPATTRAGITPATRKVLL
                     KLSGLFAIDAIGGGFLTTALLAYFFYERFGVGIEAIGVLFFMARVANALSHFAAAWLA
                     RRIGLVNTMVFTHIPSSVLLLTVPFMPTFPVAAALFLLRESLVEMDVPTRQSYVMAVV
                     RPEERTFASGITHLVRMAGWAVAPAFAGLLMTGTSLAAPLVVGASLKILYDIVLWRAF
                     RKLRPPEESG"
     gene            3472..3852
                     /locus_tag="VEW08_04865"
     CDS             3472..3852
                     /locus_tag="VEW08_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40098.1"
                     /translation="MNSELARQPNPVEQARNLLRVVFKHEQARDPRGSDAGTLERMLG
                     HLHNIEKSVTASMEDHVNASKRLRDFAETLERMKERLSTAPHQEQVSQINSLIASLRK
                     ASTELGASGAPPAWASSVPRRPAS"
     gene            complement(3901..5184)
                     /locus_tag="VEW08_04870"
     CDS             complement(3901..5184)
                     /locus_tag="VEW08_04870"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HYJ40099.1"
                     /translation="MLKPIFTALGLAACAYLTPALAHGGNSPVHRADDPAVITEWNTI
                     AEGVIPASAGVTLPRTYAMMHIAMFDAVNSIDGGYTAYRVRVPAWNGASREAAAAQAA
                     HDVLIALLPAGAPTYDAALATRLAKIHPARAQLGAQVGREVAQKILDWRSTDGWSTPQ
                     TFTPPALPGVWQPTPPAFAAAGFVQAVDAKPFALPTPYYYFPRRPPTLNSQEYADAVN
                     EIKAIGGVNSTVRTAEQTLQARLWASVGYKELWSGVWNQVSRSLARDRKLSLIESARL
                     FALVNVAMQDGVQTAQASKYAYQLWRPVHAIQRAGEDMNPATDADPTWMPLLTTPPYP
                     SYAGNMACIGASTARALALYFGTNDIPVTIQWSSTDGINYVARPFTGFWQIAEHQAAS
                     REYGGIHYHFDTMASQEVCPKVAGYIFANYMRPKR"
     gene            complement(5324..5536)
                     /locus_tag="VEW08_04875"
     CDS             complement(5324..5536)
                     /locus_tag="VEW08_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40100.1"
                     /translation="MAKGSHTIFVECRRCGHKGQREVASLPLPPEARWRCSNCQNREP
                     KSIRVWHTGGSSAARVISFPKGNRRS"
     gene            5661..8573
                     /locus_tag="VEW08_04880"
     CDS             5661..8573
                     /locus_tag="VEW08_04880"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HYJ40101.1"
                     /translation="MKFTRGRILALAGVLALVAAYALFGFFGVPRLLHSRATEFVARE
                     YGRKLEIGDIQFNPFTLVLQVHGLSFPDSEGQPLVGFRQLMVDLNVSSVFRLGPSFAA
                     IELTEPFTGLVVRKDGTLNLVDLIRPFKSPPDPAAAADEPLKLYIERFGVRSGRIDFE
                     DRARATLFKTRLAPITFELRDFATTGEAGNAYALRAASVDEERFNWNGTFETTPFTSR
                     GRFEIANLRATTLWSYLRDALPFELTSGMINVQGEYDFAARDSGLKLNVNDVTATDLS
                     LHGPNQPADDVKLAKLSITNTRFDLRQRRVDVGNVILSGAAVRARRDGQGSINLLALR
                     GADTSEPTAEVAPAASGEATPAWVLNAPDIAMEGASMDVEDGLVKPAATFKLAPIDVK
                     VTGYSNAPGTQVQIDATVGIDGNAKFAAKGQAALDTAAMSMHLEVGAFDLASLQPYLG
                     TYTQMTLSRGMLNAALDLKRNEASALGITGSVEVTKLHTVDNALKQEFITWDRLRLTG
                     LEYSPQPARLRIGTLAASAPYARFIIAPDQTTNVSKVLSAPSGSTPAPIQTVDQGDGP
                     RAASPSPLRVSIGAIRIVNGAANFADYWIQPNYAVNLQNMNGSILGLSSDPKSRAQVE
                     FEGKVDRYAPAKISGDINLLSAALYSDLKVSFKGVEMTSVTPYSGRFAGYKIEKGKLS
                     IDVAYLVENRTLSAKQRFVIDQLELGERVESEDAVRLPLKLAVALLKDRNGVIDIDLP
                     MTGSLDDPQFRMGPLIWKAFVGLISKAATAPFKLLGSLFGGGEEMNIIEFEAGSAALE
                     PAAQEKIVSIARALTERPGLQLEVPTTYSPELDRESINARRLEGLLRSLPKSDDLALA
                     DPAQRFELLMSQYRADYGTRTELPPAALALNSTRKGERAPEAIATANQELQQAITQKH
                     AVTDTDLEQLGQSRARAIQDALLGAGTLDATRVFILGANPSAPTDSKKVRLELSLK"
     gene            complement(8777..10456)
                     /gene="hutU"
                     /locus_tag="VEW08_04885"
     CDS             complement(8777..10456)
                     /gene="hutU"
                     /locus_tag="VEW08_04885"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HYJ40102.1"
                     /translation="MNRTDPRIAPGRVIRASTGTQLTAKSWLTEAPLRMLMNNLDPAV
                     AERPEELVVYGGIGRAARDWDSFDRIVETLKRLEADQTLLIQSGKPVGVFQTHEDAPR
                     VLLANSNLVPRWSTWEHFNELDRKGLMMYGQMTAGSWIYIGSQGIVQGTYETFVEMGR
                     QHYNGSLAGKWLLTAGLGGMGGAQPLASVMAGASCLAIECQRSRIDMRLKSGYLDHAT
                     DNIDEALNIIEKSCIEKKPVSVGLLGNAGEVLPDLYRRGVRPDLLTDQTSAHDPINGY
                     LPIGWTVEKWLATRITDPTTVSAAARASMASHVHSMLDFQKAGVPTVDYGNNIRQVAL
                     EEGVKNAFDFPGFVPAYIRPLFCRGVGPFRWVALSGDPEDIARTDAKVKQLLPEDVHL
                     HRWLDMARERIQFQGLPARICWVGLGDRHRLGLAFNDMVASGELKAPIVIGRDHLDSG
                     SVASPNRETEAMRDGSDAVSDWPLLNALLNTASGATWVSIHHGGGVGMGFSQHAGVVI
                     VCDGSAAAAKRIARVLWNDPATGVMRHADAGYDIALDTARKHGLDLPGILR"
     gene            complement(10453..11292)
                     /gene="hutG"
                     /locus_tag="VEW08_04890"
     CDS             complement(10453..11292)
                     /gene="hutG"
                     /locus_tag="VEW08_04890"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR02017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HYJ40103.1"
                     /translation="MNAPAHLEWLAIHHGEAPLLVSFPHTGIEIPADIESNLVSPWLA
                     RKDADHWVDVLYDFAHEMGATTLRTAMSRTVIDVNRDPSGASLYPGQATTGLCPIETF
                     DGEPLYAAGHEPDAAEIARRRAAYFDPYHAALNSQLQRLRARHARVVLFDAHSIRSEV
                     PRLFDGTLRQFNIGTNNGATCDAALTARIEKICEASSFDHVTNGRFRGGWITRHYGKP
                     QNGIHAVQMELAMRGYLHEPEEKLSEANWPAPLEDAHAATLRVALRDILDTCVTFAAG
                     KST"
     gene            complement(11289..12491)
                     /gene="hutI"
                     /locus_tag="VEW08_04895"
     CDS             complement(11289..12491)
                     /gene="hutI"
                     /locus_tag="VEW08_04895"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HYJ40104.1"
                     /translation="MHCDRIWRNAHLATLVGNQGLGLVTDGLIACRGGLITFAGPARD
                     AQELTAAEDIDCQGRLITPGLIDCHTHLVYAGNRAHEFEQRLEGHSYADIASKGGGIL
                     STVNATRAASEDQLVAEALPRLDALLAEGVTTIEIKSGYGLSLEHERKQLRAARELAR
                     KRRVNVSTTFLGAHALPPEFAGRADDYITEICANMLPALVKEGLVDSVDAFCEHIGFS
                     LEQTKRVFNAANLQGLRVKLHAEQLSNMHGAVMAAAFRALSADHLEFVDEAGIEAMRD
                     AHTAAVLLPGAFYFLRETRLPPIDLLRRHGIAMAIATDCNPGTSPLTSLLMAMNMAAT
                     LFRLTVDECLIGVTRAAAQALGLARSRGTLEPGKQCDLAIWNVGRPAELVCAMGSNPL
                     HARVWKGE"
     gene            12579..13118
                     /locus_tag="VEW08_04900"
     CDS             12579..13118
                     /locus_tag="VEW08_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40105.1"
                     /translation="MKTLLMLVMFATLSASAANDPPANPANDRWGRVRFLLGTWEGTA
                     NGEPGKGTVERTYTLVLGGQFIEEHNTSRYEAKAGQKPEIHHHRGFLSYDKARKTFML
                     RHFHEEGFVNLYALDADKSMPEYLIFDSVSFENFSNEWKARESYEVISPDEFVEVFEL
                     AEPGKDFVEYSRVHFLRKK"
     gene            13123..14487
                     /locus_tag="VEW08_04905"
     CDS             13123..14487
                     /locus_tag="VEW08_04905"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006683.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HYJ40106.1"
                     /translation="MAFHFASALLPEGWAQNVRVEIAGGRFTKLERDVQPREGDDRVA
                     VALPGMCNVHSHGFQRGMAGLTEFRGPEADNFWSWRTLMYRFVARMTPEDIGAITAQS
                     YVEMLESGFTRVGEFHYVHHDPAGNPYVNPAETAIHVASAVARTGIGLTLLPVFYAHG
                     GFDGAKPNDGQRRFVTGLDEFARLMEASRKIVGSLEGANLGVAPHSLRAVTPEELARV
                     VPLAGSGPVHIHAAEQTGEVEQCLAWSGARPVQWLLDHAAVDRRWCLVHSTHVTDEEC
                     GRLAAIGAVAGLCPITEANLGDGIFPAPTYLGAGGAFGIGTDSNVSVGMADELRQLEY
                     SQRLRDRARNVVGAADARSTGRVLFEGAIRGGAQALGIGPVIGIAQDAAADFVSLNPE
                     APALGARDGDALLDSLIFAGGAGCIDGVWRAGRKLVADGQHLERRAIAQAYQATLRRL
                     LQQD"
     gene            14680..16857
                     /locus_tag="VEW08_04910"
     CDS             14680..16857
                     /locus_tag="VEW08_04910"
                     /inference="COORDINATES: protein motif:HMM:NF045042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase"
                     /protein_id="HYJ40107.1"
                     /translation="MERLSRGVVAVRTSSSQVYVGWRLFGNEGSGTSFNLYRSSNGGT
                     PMLLNGTPLTSSTNFVDTSAPPTQSNAYTVRAVVNGVEQSASAAFTLPANAPTRQYLE
                     IPLQIPGGGTTPSGEAYTYTANDLSVGDLDGDGELEYVLKWDPTNAKDNSQSGYTGNV
                     FLDAYELNGTRLWRIDLGRNIRAGAHYTQFQVYDFDGDGKAEVMCKTADGTRSGTGQV
                     IGSSSADHRNSSGYILAGPEFLTVFNGQTGAVLATTNYVPARGNVGDWGDTYGNRVDR
                     FLAGVAYLDGQRPSAIFSRGYYTRTVIAAWDYRNGSLTQRWVFDSNSAGSTYEGRGNH
                     NLSVADVDNDGRHEVMFGSLGINDNGTAMWATNHHGDALHVSDFIPGRAGLELFKPSE
                     HTNELADYMLDARTGSIIWSHPTCGCDNGRGVAGDIYAGSAGAEAWSSAVAGLSNTSG
                     GQASSRKPGSTNFLIWWDTDTLRELLDSNHVDKYGTGGDTRLLTADGTTSNNGTKSTP
                     ALSGDLFGDWREELVLRTTGNNALRIYTTTNVATNRIYTLLHDPQYRVAIAWQNTGYN
                     QPPHPSFFIGNGMSTPPTPNIVLVGGAPPDPQPGTPTYQLETASLGGGTVSESTNGGF
                     MGSGYANFSATGGFAQVNNADGRGGGSHAMRIRYALGATASRTGRVLVNGVAQNITFN
                     PTGGWTTWATQNVTFTFNNGTVNTIRFESNGQDLANVDQIEIL"
     gene            complement(16923..18305)
                     /locus_tag="VEW08_04915"
     CDS             complement(16923..18305)
                     /locus_tag="VEW08_04915"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HYJ40108.1"
                     /translation="MTPPPAITFVNASVHDPSVIKVGAEYYVFGSHLAAARTTDLMNW
                     TLVADGVNNANPLFTNVLTALQQTFAWSTVNDLWAPDVVKLADNKFYFYYNSCRGDSP
                     LSALGVAVADSINGPYTNRQIILRSGMAGLSEDGVTNYDAQVHPNVVDPQTFFDATGH
                     LWMIYGSYSGGIFILAMDETTGLPEPGQGYGRHLLGGNHARIEGAYVLYNAQTGFYYM
                     FTSFGGLAANGGYNIRVARSQAPDGPYFDAAGTNMDTVRGNPAVLFDDTRIAPHGQKL
                     MGNFQFSLATGETGTAEGYVSPGHNSAYYDAALDKYFLIFHTRFPARGEQHELRVHQL
                     MFNSAGWPVAAPFRYAPLSLSPTAVGADVTNADVAGAYKLIDHGDDISATIKTSQAIR
                     LNADGTMTGAMTGTWLHRGNNLIDIALSGVIYNGVLSRQWNTNANRFVVTFSVQAQHG
                     VSLWGARTGD"
     gene            complement(18507..>18718)
                     /locus_tag="VEW08_04920"
     CDS             complement(18507..>18718)
                     /locus_tag="VEW08_04920"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1993 family protein"
                     /protein_id="HYJ40109.1"
                     /translation="SDFDGAATRVVSQPRWKGEWMTGADYFVQHSVPNFFFHLATAYA
                     ILRHNGVSIGKRDFLGAQTKRPPAN"
BASE COUNT         3330 a         5889 c         6128 g         3371 t
ORIGIN      
        1 gaacatgccg ggggtgaaac gccggtcggc gttcttgaac agtccgcgcg cgtgaatggt
       61 gcccgtgttc gggtcgagct cgttgtcgag gaagaccatg acgccttcgt gcggatggcc
      121 ctgttcattg gcgcttccga cgcacaccgg attttgcgtg tcgcgcgagc tgccacgttc
      181 gccgcgcagg gccatgtcga cgtacttgag gtatacctgc tcgtcgccgt cgaacgagac
      241 gtagatggga tcgatcgaca ccaccgtggt cagcagagtc tggcctgcag cgacgaggtt
      301 accggcggtg atctcggctc ggcccacaag gcccgagatc ggcgcgcgca cctcggtgaa
      361 tgacaggtcg agcgcggcgg attccaccgc cgcttcggac gcctggagat tggcggcggc
      421 ctgctcgttg cccgagatgc gcgcatcgaa ttcctcttgc gagatcgcgc gcttttcaat
      481 gagcttctgg gcgcggacgc gctcggactc ggccagtgcg gcctgggtgc gtgcgcgcgt
      541 cagctcggcc tgcgcgtgtt tgagagtggc ctggtagggg cggggatcga tgacatagag
      601 aatgtcgccc ttcttcacct cgtggccctg cgcgaagcgc gtcgccatca catagccgga
      661 aacgcgcggc cgcacctcga ccctttcaac ggcttcaaag cggccggtga actcgtcgaa
      721 ctcggtgatc gacttcgaga cgaccatggc ggcctgcacc ggtggcggag cggctggggt
      781 ttcggcggct tcattgcgcg cgcagccagc gagaatgaga gcggccagag gaagaatgaa
      841 agcgggggcg agtgatgact tgtcgatggg cacggggaat ctcccttcag gttgtgaggc
      901 atatcctccg ctcccgcgcc tcgccgcaca ttgaccgggt gaggcacgga ctggggctat
      961 tgcggaaggg cgtgacctac ccctttcggg gtagaacgcg gagcgcgcgc aagagagaat
     1021 gtcatcggcg cacgaacccg gtgctgactt gtccgtaaca cgagttaagg aattctcaac
     1081 gatgccaacc cacatggaga tcgtgctgca ggccgcggcc ttcgcggcgg agaagcaccg
     1141 caaccagcgc cgcaaggacg cggaagcctc gccctacatc aatcatccga tccagctcgc
     1201 gtacatcctc gtgcaggcgg acgtcgagga tcccatcgtg ctggccgccg cgctgctgca
     1261 cgacacgatc gaagacacga acaccacgca cgatgagatc gagatcgtgt tcggtcacga
     1321 gatcgcgaac atcgtcgtcg agtgttccga cgacaagact ctcgacaagc tcgagcgcaa
     1381 gcaggcgcag gtggatcacg ccgcgcacat cagcaatcgc gccaagctcg tgaagctcgc
     1441 cgacaagatc gccaatgtga gcgacatcga cggcgcgccg cccgccggct ggtcgctcga
     1501 gcgcaagcgc gaatacttcg attgggccaa gcgcgtggtg gataagatgc gtggcgtgca
     1561 cccaaagctc gaagctcgtt tcgacgcgga atacgccaag cgcccatgac ctccatcgtt
     1621 cgatggctgc tgctcgccaa tgtcgccgtg ttcgggctgc aacagctctc ccgcgatgag
     1681 ttgctgatcc attttgcgtt gtggccgctc ggggccggtt tcgagccctg gcagctcgtc
     1741 acctcggcat tcctgcacga caatcttccg cacatcgcac tcaacatgta cgcgttgtgg
     1801 atgttcggca gcatggtgga acgtgtgctc ggacccgggc gattcgcctg ggtctacctc
     1861 gcatcggttc ttgcggggag cctggttcaa ctgctcgtgg tgacgatggc actcgatcgg
     1921 gggatcaccc ccacggtcgg agcctcgggc ggagtgttcg gtattttgct ggcattcgcc
     1981 atgctgtttc cgcacagccg cctgtacatc attccggtgc cggttcctct caaggcatgg
     2041 gtggtggtcg ccggttacgc ggccatcgaa ttggcgagcg gcgtcatggg cacgagccag
     2101 ggtgtggcgc acttcgcgca tctcggcggc atgttaggtg ccgctgtcgt gatgctcgcc
     2161 atcggttttc ggaaaagtgt gcagccgccc ggagatctgc gcggtcctta gaaacacatg
     2221 aacgccggac acccgcacga ccgccagtgg ctgtacgccg ccgtactggc gcgctccgtg
     2281 gccaccggca tgatgggtgt gttgcttggc atctatctcg cgagtctggc actcgacgcg
     2341 aatgccatcg gtgttgtcat cggcctcggg ctggccggcg ccgcattcgg cacactgctg
     2401 gtcacgtatt tttcggatcg cttcggccgg cggcgaacgc tgttcgtcat caccgcgctc
     2461 accgtgtgcg gcgcggtgat gctggccttc tcgatgtcgc cgctgaccct gggactcgcc
     2521 gcattcctcg gcatggtcaa cggcatgggg cgcgaccgcg gcgccgcatt ggtggtggag
     2581 cagtcggtgc tgcccgccac cgtcaccgac agccagcgca cgatggcgtt tgcgaagtac
     2641 aacgtattgc aggacgtcgg tcacgcggca gggtcgctgc tcgccgcggc cccggcattg
     2701 tttcagcgat tcacgttgct cgatgccgcg cactccctga aagcgtcgat cttcgtctac
     2761 gtggcgttgt cactgttgcc gttcctggcc tatctgcgcc tttcagatcg cgtcgaggtt
     2821 cccgcaacta cacgagcggg gatcaccccc gccacacgca aggttctgct caagctcagc
     2881 gggctgtttg ccatcgatgc catcggcggc gggtttctca ccaccgcgtt gctggcgtac
     2941 ttcttctacg agcgcttcgg cgtcggcatc gaggccatcg gcgtgctgtt cttcatggcg
     3001 cgcgtcgcca atgcgctatc gcatttcgca gcggcctggt tggcgcggcg catcggtctc
     3061 gtgaacacga tggtgttcac ccacatcccc tcgagcgtgc tgctgctgac cgtgcctttc
     3121 atgccgacct ttccagtggc cgcggcgctg ttcctgttgc gtgaatccct ggtcgaaatg
     3181 gatgtgccga cgcgccagtc gtacgtcatg gcggtggtac gcccggaaga gcggaccttc
     3241 gcttcgggca tcactcacct ggtgcgcatg gccggctggg ccgtggcgcc ggcgtttgcc
     3301 ggcctcctga tgaccggcac ttcgttagct gcacctctgg tggtgggtgc gagtttgaag
     3361 atcctctatg acatcgtgct gtggcgggcc ttccggaagc tccgtccgcc tgaggaatcc
     3421 ggctgaaatt ctgatagaca acctcgcgcg ccgctgccag tgtgcgggct aatgaacagt
     3481 gaactcgccc gacagcccaa tccggtggaa caggcccgaa acctgctccg cgtggtcttc
     3541 aaacacgaac aggcgcgcga tccgcgtggc agtgacgcag gaacgctgga gcgcatgctc
     3601 gggcacctgc acaacatcga gaaatcggtc accgcgtcca tggaagacca cgtcaacgcg
     3661 tcgaagcgcc tgcgggattt cgcggaaacg ctggagcgca tgaaagagcg cctatcaacc
     3721 gctcctcacc aggagcaggt atcgcagatc aactcattga tcgcatcgct gcgcaaggcc
     3781 agcaccgagc tcggcgcctc tggtgccccg ccggcctggg ccagcagcgt gccgcgacgc
     3841 cctgcgagct gaaagaaaaa aaggcgcccc gtgaggggcg ccaatgtcag ggaagcgtgg
     3901 ttatcgcttc ggtcgcatgt agttggcaaa gatgtatccg gccaccttcg ggcagacttc
     3961 ctggctcgcc atcgtatcga agtgatagtg aatgccgccg tactcacggc tggccgcctg
     4021 gtgctccgcg atctgccaga agcccgtaaa cggacgcgcc acataattga ttccatcggt
     4081 gctcgaccat tgaatcgtga ccgggatgtc attggtgccg aagtacagcg ccagcgcgcg
     4141 ggccgtgctc gctccgatgc aggccatgtt gccggcatag gacggatagg gcggcgtagt
     4201 tagcagcggc atccaggtcg gatcggcgtc ggtcgccgga ttcatgtcct cgccagcgcg
     4261 ctggatggca tgcaccgggc gccagagctg gtaagcgtat ttgctggcct gcgcggtctg
     4321 cacaccatcc tgcatggcga cattcaccag cgcgaacagg cgggcggact cgatcagcga
     4381 cagcttgcga tcgcgcgcca gggaacgcga gacctggttc cacacgccgc tccacagttc
     4441 cttgtagccg acgctggccc agagacgcgc ctgaagcgtc tgctctgccg tgcgcacggt
     4501 gctgttgacg ccgccgatcg ccttgatctc attcacggca tccgcgtact cctggctgtt
     4561 cagtgtcggg gggcggcgcg ggaaataata ataaggagtg ggcagcgcga acggcttcgc
     4621 atcgaccgcc tgcacgaaac ctgccgccgc gaaggccggg ggcgtcggtt gccacacgcc
     4681 cggcagcgcc ggcggggtga acgtctgggg cgtcgaccag ccgtcggtgc tgcgccaatc
     4741 gaggatcttc tgggccactt cgcgtcccac ctgcgcgccg agctgtgcgc gtgcgggatg
     4801 gatcttcgcg aggcgggtgg cgagcgcggc gtcgtaggta ggcgcgcctg ccggcagcag
     4861 ggcgatcagc acgtcgtgcg cagcctgggc ggcagccgct tcacgagagg cgccgttcca
     4921 ggccggcacg cgcacgcgat aggcggtgta tccgccgtct atggagttca ccgcatcgaa
     4981 catcgcgatg tgcatcatcg cgtaggtcct tggcagcgtg acgccggcgg aagccggaat
     5041 gactccctcg gcaatcgtgt tccattcggt aatcaccgcc ggatcgtccg cgcgatggac
     5101 cggtgagtta ccgccatgcg caagcgcagg cgtaaggtag gcgcaggccg caaggcccaa
     5161 cgccgtgaat atcggcttca acatgatcgt ctccaaactg aggtgtgatt gacggcgcga
     5221 acgatgcgcg tgtcacgtgg aagccggcgt ggtagtcgcc gtggaattta tcaggatggg
     5281 gtcaggcacc atttgccagg aatcggtgcc tgacaccatt tttctaacta cggcggttgc
     5341 ccttcggaaa cgagatgacg cgcgccgcag acgacccgcc cgtgtgccac accctgatgg
     5401 acttgggctc gcggttctgg cagttcgagc agcgccatcg cgcctcgggc ggcagcggca
     5461 gggatgcgac ttcgcgctgc cccttgtgcc cgcagcggcg gcattcgaca aagatggtgt
     5521 gggagccttt ggccatgccg ccgagcgtac tttgcgacac aatgtcctca gcaagcgcgg
     5581 gaccgcggca ctgtataagc ccttggcccg atgcgtgaat gctaaaagca gggtgtaatc
     5641 gcgggcgtat ggtggcgttc atgaagttca cgcgcggccg gatcctggcc ctcgcgggcg
     5701 tgctcgcgtt ggtcgcggcg tatgcgctgt tcggcttctt cggcgtaccg cgcctgttgc
     5761 actcccgcgc caccgagttt gtcgcccggg agtacggccg caagctcgag atcggcgaca
     5821 tccagttcaa tcccttcacg ctggtgctgc aggtgcacgg cctgtcattc cccgacagcg
     5881 agggccagcc gctggtgggt ttccgtcaat tgatggtcga tctgaatgtg tcttcggtgt
     5941 ttcgcctggg cccgagtttc gctgccatcg aactcaccga gccgtttacc ggcctcgtcg
     6001 tccgcaagga cggcacgctc aatctcgtgg atctcatccg gccattcaaa tctccaccgg
     6061 atccggccgc ggcggcagac gaaccattga agctgtacat cgagcggttc ggcgtaaggt
     6121 cgggacgcat cgacttcgaa gaccgggcac gcgccacgtt gttcaagacg cgccttgcgc
     6181 ccatcacctt cgagctgcgc gatttcgcca ccacgggcga agccggcaac gcgtatgcct
     6241 tgcgggccgc gtcggtcgac gaggagcgct tcaactggaa tggcacattc gagaccacgc
     6301 cgttcacctc gcgtggccgg ttcgaaatcg cgaacctgcg cgccacgacc ttgtggtcct
     6361 acctgcgcga tgcgctgccc ttcgagctca cgagcggcat gatcaatgtt cagggcgaat
     6421 acgacttcgc ggcgcgcgat tcgggcctga agctgaacgt gaatgacgtg accgcgacgg
     6481 atctgtcgct gcacggtccg aaccagcccg cggacgacgt gaagctcgcg aagctttcca
     6541 tcacgaatac ccgctttgac ctgcggcaac ggcgcgtcga tgtcgggaat gtgattctgt
     6601 cgggtgccgc ggtgcgcgcg aggcgcgacg ggcagggcag catcaacctg ctcgcgctgc
     6661 gcggcgccga cacgtcggag cccacggccg aagtcgcgcc cgcggcttcc ggcgaagcca
     6721 ctccggcgtg ggtgctgaac gcgccggaca tcgccatgga aggcgccagc atggatgtgg
     6781 aagacggcct ggtgaaaccg gcggccacgt tcaagctcgc acccatcgac gtgaaggtga
     6841 ccggctacag caacgcgccc ggcacacagg tgcagatcga tgcgactgtg ggcatcgatg
     6901 gcaacgcgaa gtttgccgcc aagggccagg cggcgctgga tacggcggcc atgtccatgc
     6961 acctggaggt tggggccttc gatctcgcct cgttgcagcc gtatctgggc acgtacacgc
     7021 agatgaccct ctcccgaggc atgctcaatg cggcactcga tctcaaacgc aacgaagcga
     7081 gtgcgctcgg catcacgggc tccgtcgagg tgacgaagct gcacaccgtc gacaacgcgc
     7141 tcaagcagga attcatcacc tgggatcgcc tgcgtctcac cgggctcgaa tattctccgc
     7201 agcccgcgcg gctgcggatc ggcaccctgg cggccagtgc gccgtacgcg cggttcatca
     7261 tcgcgcccga ccagactacc aacgtcagca aggtcctcag cgcaccttcg ggcagtacac
     7321 cggcaccgat ccagacagtg gaccagggcg acggacccag ggctgcatcg ccgtcaccgc
     7381 tgcgcgtgag catcggcgcg attcgcatcg tcaatggcgc cgccaacttc gccgactact
     7441 ggattcagcc caactacgcc gtgaatctgc agaacatgaa cggcagcatt ctcgggctgt
     7501 cgtcggatcc caagtcgcgc gcccaggtgg aattcgaagg caaggtggat cgatatgcgc
     7561 cggcgaagat ttcgggcgac atcaacctgt tgtcggcggc gctgtacagc gatctcaagg
     7621 tgagtttcaa aggcgtggag atgacgtccg tgacgccgta ttccggccgc ttcgcgggct
     7681 acaagatcga gaagggcaag ctttccatcg acgtggcgta tctcgtcgag aatcgcacgc
     7741 tctccgcaaa gcagcgcttc gtcatcgacc agctcgagct cggcgagcgc gtggagagcg
     7801 aagatgccgt gcggctgccg ctcaagctcg ccgtggcgct tctgaaagat cgcaatggcg
     7861 tcatcgacat cgacctgccc atgacgggca gcctcgacga tccgcagttc cgcatgggtc
     7921 cgctcatctg gaaggcgttc gtgggtctca tttccaaggc cgcgaccgcc ccgttcaaac
     7981 tactcggcag cctgttcggc ggtggcgagg aaatgaacat catcgagttc gaggccggca
     8041 gcgccgcgct cgagccggcg gcgcaggaaa agattgtctc catcgccagg gcgctgaccg
     8101 agcggcccgg cctgcagctg gaggtgccga ccacctattc gccggagctc gatcgcgaat
     8161 cgatcaatgc gcgaagactc gaaggcctgc tgcgttcact gccgaaatcg gatgatctgg
     8221 cgctggcgga tccggcgcag cggttcgaat tgctcatgtc gcagtaccgc gccgactacg
     8281 gcaccaggac ggagttgccc ccggccgcgc tggcgctcaa ttccacgcgg aagggagaac
     8341 gtgcgccgga ggcgattgcg accgcgaacc aggagctcca gcaggcaata acgcagaagc
     8401 acgcggtcac cgatacggat ctcgagcagc tcggccagtc gcgcgcccgc gcgattcagg
     8461 atgcgttgct gggggcgggt acgctcgacg cgacccgcgt cttcatcctg ggcgccaacc
     8521 cttcggcgcc cacagacagc aagaaggtgc gcctggagct gagcctgaag tagcggggcg
     8581 ctttcgcgcc ccacggccga tcagggtcgc tgcaggaatc cacgctcggc tttcttggct
     8641 ttcttcgccg cgcgcttttc gtcgggcgtt ttcgcggcct tcttcttctg gttcttcttc
     8701 tggtccattc ccttgctcat ggccgtcgct ccagtgttgt gagaagtcgc gagactagca
     8761 gccattggcg ttggcgtcat cgcagaattc ccggcagatc gagcccatgt ttgcgcgcgg
     8821 tatcgagtgc gatgtcatag cccgcgtcgg cgtggcgcat cacgccggtc gccggatcgt
     8881 tccagagtac gcgcgcgatg cgtttggcgg cggccgcgga cccatcgcac acaatgacaa
     8941 cgccggcgtg ctgcgaaaat cccatgccca cgccgccgcc atgatgaatc gaaacccagg
     9001 tggcgcccga tgccgtgttc aacaacgcgt tgagcagcgg ccagtccgag accgcgtccg
     9061 atccgtcgcg catggcttcg gtctcgcggt tgggggaggc gaccgaacct gaatcgaggt
     9121 gatcgcggcc gatgacgatg ggcgccttga gctcgccgct cgccaccatg tcgttgaatg
     9181 ccagccccag ccggtgccgg tcgccgagcc ccacccagca gatgcgcgcg ggcaggcctt
     9241 ggaactggat gcgttcgcgc gccatgtcga gccagcggtg caggtgcacg tcttcgggca
     9301 ggagttgctt gaccttggcg tcggtcctgg cgatgtcttc gggatcgccc gacagcgcca
     9361 cccagcggaa cggcccgacg ccacggcaga acagcggacg aatgtatgcc gggacgaaac
     9421 cgggaaagtc gaatgcgttc ttcacacctt cttcgagcgc cacctggcgt atgttgttgc
     9481 cgtaatccac ggtgggtacg ccggcctttt gaaaatcgag catggaatgc acgtgcgatg
     9541 ccatggatgc cctggctgcc gcgctcacgg tggtgggatc cgtgatgcgg gtcgccagcc
     9601 atttctccac ggtccagccg atgggcagat agccgttgat gggatcgtgg gccgaggtct
     9661 gatccgtcag caaatccggg cgaacgccgc ggcggtaaag gtcaggaaga acttcgcccg
     9721 cattgccgag cagtccgacg gaaaccggct tcttctcgat gcaggatttc tcgatgatgt
     9781 tcagcgcctc gtcgatgttg tccgtggcgt gatcgaggta gccggatttc aaacgcatgt
     9841 cgatgcgtga gcgctggcat tcgatggcga gacatgacgc gcctgccatg accgacgcca
     9901 gcggttgcgc gccgcccatg cccccgagcc cggcggtgag cagccacttg cccgcgagac
     9961 tgccgttgta gtgctgccgc cccatctcga cgaaggtttc gtacgtgccc tgaacgatgc
    10021 cctgcgagcc gatgtagatc cacgatccgg ccgtcatctg gccgtacatc atgagtccct
    10081 tgcgatcgag ctcgttgaaa tgctcccagg tgctccagcg cggcacgagg ttggagttgg
    10141 ctaacaacac gcgcggcgca tcttcgtggg tctggaagac gccgaccggc ttgccggact
    10201 ggatgagcag cgtctgatcg gcctcgagtc gcttcagggt ctcgacgatg cggtcgaagc
    10261 tgtcccagtc gcgtgccgcg cggccgatgc cgccatagac cacgagttcc tccgggcgct
    10321 ccgccacggc cggatcgagg ttgttcatga gcatgcgcag cggcgcttcc gtgagccagc
    10381 tttttgccgt cagttgtgta ccggtcgaag cgcggatgac ccggccagga gcgattcttg
    10441 gatcggtgcg attcatgtgg atttaccggc agcaaaagtg acgcaggtgt ccagaatatc
    10501 gcgcagcgcg acgcgaagcg tggcggcatg cgcatcctcg agcggagccg gccagttcgc
    10561 ttcggacagc ttttcttccg gctcatgcag gtagccgcgc atcgcaagct ccatttgtac
    10621 cgcatgaatg ccgttttgcg gcttgccata gtggcgcgtg atccatccgc cgcggaagcg
    10681 cccgttggtg acatggtcga acgacgacgc ttcgcagatc ttctcgatgc gcgcggtcag
    10741 tgccgcatca caggttgcgc cattgttcgt cccgatgttg aactgccgca gcgttccgtc
    10801 gaacagtcgc ggtacctccg agcgaatcga atgtgcgtcg aacagcacca cgcgcgcgtg
    10861 gcgtgcgcgc agtcgttgca gctgggaatt gagcgcggcg tgataggggt cgaagtacgc
    10921 ggcgcgccgg cgggcaattt ccgcggcgtc tggctcgtgt ccggctgcgt acagcggttc
    10981 accatcgaag gtttcgatgg gacacaggcc cgtcgtggcc tgcccggggt acagcgaagc
    11041 gccggacgga tcgcgattca cgtcgatgac ggtgcgcgac atcgccgtgc gcagcgtggt
    11101 ggcgcccatc tcgtgtgcga agtcgtagag cacgtcgacc cagtggtccg cgtctttgcg
    11161 cgccaaccag ggtgagacga ggttcgattc gatgtccgcg gggatctcga tgccggtgtg
    11221 cgggaagctg accagcagcg gggcttcacc gtggtgaatc gccagccatt cgaggtgcgc
    11281 gggggcgttc attcgccttt ccatacacgc gcatgcagcg gattggaacc cattgcgcag
    11341 accagctccg ccggccgccc gacattccag atggcgagat cgcattgttt gccaggttcg
    11401 agcgtgccgc ggcttcgtgc cagcccgagc gcctgcgcgg ccgcgcgcgt gacaccgatg
    11461 aggcactcgt cgaccgtcag gcgaaacagc gtggccgcca tgttcatcgc catcagcagg
    11521 gaagtgagcg gcgaggtgcc cgggttgcag tccgtggcga tggccattgc gatgccgtgc
    11581 ctgcgcagca ggtcgatggg tggcagtcgc gtctcgcgca ggaagtagaa agcaccgggc
    11641 agcagcaccg ccgcggtgtg cgcatcgcgc atggcctcga tgccagcctc gtcgacgaat
    11701 tcgaggtgat ccgcggacaa cgcgcgaaag gcggcggcca tgaccgcacc gtgcatgttc
    11761 gagagctgct cggcgtgcag ctttacgcgc aggccctgga gattcgcggc gttgaaaacc
    11821 cgcttggtct gttcgagcga gaagccgatg tgctcgcaga acgcatcgac cgaatcgacc
    11881 agtccttcct tcaccagcgc cggcagcatg ttcgcgcaga tctccgtgat gtagtcgtcg
    11941 gcgcggccgg cgaactcggg aggcagcgca tgcgcgccga gaaacgtggt actcacgttg
    12001 acgcgacgct tgcgcgccaa ctcgcgggcc gctcgaagtt gtttgcgctc gtgctcgagg
    12061 ctgaggccat agccggactt gatttcgatg gtggttacgc cttcggcgag cagtgcatcg
    12121 agccgcggca acgcttcggc cacgagctga tcttcactcg cagcgcgcgt cgcattcacc
    12181 gtcgacagga tgccgccgcc tttgctggcg atatccgcat aggagtggcc ttccaggcgc
    12241 tgctcgaatt cgtgggcgcg attgccggcg tagaccaggt gcgtgtggca gtcgatgagg
    12301 cccggcgtga tgaggcggcc ctggcagtcg atgtcttctg cagccgtaag ctcctgcgca
    12361 tcgcgcgcgg ggcctgcaaa agtaatgagt ccgccccggc aggcgatgag gccgtcggtg
    12421 acgagcccaa gtccctggtt gcccacgaga gtcgcaaggt gagcgttgcg ccagatgcgg
    12481 tcacagtgca tgccggaacc atatagatga gtggcgcggg ccgccagagg cgcggagcca
    12541 gcgcatatac tgcggctaca cgcaggaggg agcccgcgat gaagactctg cttatgctcg
    12601 tcatgtttgc gacgctgtcg gccagcgccg cgaacgatcc gccggccaat ccagcgaacg
    12661 atcgctgggg ccgcgttcgc ttcctgctcg gcacctggga aggcaccgca aacggagagc
    12721 cgggcaaggg caccgtcgag cggacctaca ccctggtgct cggtgggcag ttcatcgaag
    12781 agcacaacac cagccggtac gaggcgaagg caggtcaaaa acccgagatc caccatcacc
    12841 gcgggttcct gagctacgac aaggcgcgca agacattcat gcttcgccac ttccacgagg
    12901 aaggcttcgt caacctgtat gcgctcgacg ccgacaagag catgccggag tacctgattt
    12961 tcgacagcgt gagcttcgaa aacttcagca atgaatggaa ggcgcgcgaa agttacgagg
    13021 tgatctcgcc cgacgagttc gtcgaggtct tcgagcttgc cgagccgggg aaagatttcg
    13081 tcgaatacag ccgcgttcac tttctacgca agaaataacc acgtggcatt tcatttcgca
    13141 tccgcgttgc tgcccgaagg ctgggcgcag aacgtccgcg tcgaaatcgc gggtggacgc
    13201 ttcacgaagc tggagcgcga tgtgcaaccg cgcgagggtg acgatcgcgt cgccgtggcg
    13261 ttgcccggca tgtgcaacgt gcatagtcac ggctttcagc gcggcatggc gggcctcacg
    13321 gaattccgcg gtccggaggc agataacttc tggagctggc gcacgctgat gtatcgcttc
    13381 gtggcgcgca tgacgcccga agacatcggc gccatcacag cccaatcgta cgtcgagatg
    13441 ctcgagagcg gattcacgcg cgtgggcgag tttcactacg tacaccacga tccggcgggc
    13501 aacccgtacg tgaatccggc ggagaccgcg atccacgtgg cctccgcggt cgcgcgcacc
    13561 ggcatcggac tcacactgtt gccggtgttc tacgcgcatg gcggattcga cggcgcgaag
    13621 ccgaacgatg gtcagcgccg cttcgtcacc ggcctcgacg agttcgcgcg actgatggag
    13681 gcgagccgga aaatcgtcgg ctcgctcgaa ggcgcgaatc tcggtgtggc tccgcattcg
    13741 ttgcgtgcgg tcactcccga ggaactggcg cgggtcgtgc cgctcgcggg ctctggtccg
    13801 gtccacattc atgccgccga acaaaccggc gaggtggagc agtgcctggc ctggtcgggt
    13861 gcgcgacccg tgcagtggct gctggaccat gccgcggtcg accggcgctg gtgcctggtg
    13921 cattcgacgc acgtcacgga cgaggagtgc ggccgtctcg cggctattgg tgccgtcgcc
    13981 ggcctgtgtc ccatcaccga agcaaacctg ggtgacggga ttttcccggc tccgacatac
    14041 ctgggcgccg ggggtgcgtt tggcatcggt acggattcca atgtgtcggt cggcatggcc
    14101 gacgagttgc gccagctcga gtacagccag cgcctgcgcg accgtgcccg taacgtcgtc
    14161 ggcgccgcag atgcgcgttc gaccggtcgc gtcttgttcg aaggtgcaat tcgtggcgga
    14221 gcgcaggcac tcggaatcgg accggtcatc ggtattgccc aggacgccgc ggccgatttc
    14281 gtttcgttga atccggaagc cccggcgctc ggggcgaggg acggcgatgc gttgctcgac
    14341 agcctgattt tcgctggcgg cgccggttgc atcgacggcg tgtggcgggc gggtcgcaaa
    14401 ctcgtggcgg atggccagca tctcgaacgc cgcgcgatcg cgcaggccta tcaagcgacg
    14461 cttcggcgcc tgctacagca ggactgaccg aactacggat tgacaccggt atcactgcac
    14521 gtatattggc gcgcgctgag gggggacgat cgagcgcggc cagggatatg gcgtgtgaac
    14581 gcccaaggac ggccaatgaa attctcaagg actaacaccg cggcggcatc agttgccgtg
    14641 cttcttgctt gtgcctttca ggcacaagcg caataccaga tggaaaggct gtcgcgcggc
    14701 gtggttgccg tgcgcaccag cagttcgcag gtctacgtcg gctggcgact gttcggaaac
    14761 gaaggctccg gtacgtcgtt caacctgtac cgctcgagca atggcggaac accgatgttg
    14821 ctgaacggca caccgctgac ttcatccacc aacttcgtcg acacttccgc accgccgacc
    14881 cagtcgaacg cgtacaccgt gcgcgccgtg gtcaatggcg tggagcagag cgccagcgct
    14941 gcgttcacgc tgccggcgaa tgcgccgacc cggcaatacc tcgaaattcc gctgcagatt
    15001 cccggtggtg gcacgacgcc gtcgggcgag gcgtacacct acacggcgaa cgacctgtcc
    15061 gtcggcgatc tcgacggcga cggcgagctc gaatacgtgc tcaagtggga tccgaccaac
    15121 gccaaggaca attcgcagag cgggtacacg ggcaatgtgt tcctcgacgc gtatgagctc
    15181 aacggcacgc gtctgtggcg catcgatctc ggccgcaaca tccgcgccgg cgcgcactac
    15241 acacaattcc aggtgtacga cttcgacggc gacggcaagg ccgaagtcat gtgcaagacc
    15301 gccgatggca cgcgcagcgg caccggccag gtcatcggca gctcgagcgc ggatcatcgc
    15361 aattcgtcgg gctacatcct tgccggcccg gaattcctga ccgtgttcaa cggtcagacc
    15421 ggcgccgtcc tcgccaccac taactacgtg ccggcgcgcg gcaatgtggg tgactggggc
    15481 gacacgtacg gcaaccgcgt cgaccgcttc ctcgcgggcg tggcgtatct cgacggccag
    15541 cgtccgagcg ccatcttctc gcgcggctac tacacgcgca ccgtgatcgc cgcgtgggat
    15601 taccgcaacg gctcgctgac ccagcgctgg gtcttcgaca gcaactcggc ggggagcacc
    15661 tacgagggcc ggggcaatca caacctgagc gtcgccgacg tcgacaacga cgggcgccac
    15721 gaggtcatgt tcggctcgct ggggatcaac gacaacggta ccgcgatgtg ggccaccaac
    15781 catcacggcg acgccctgca tgtgagcgac ttcattcccg gccgcgccgg gctcgagctg
    15841 ttcaagccga gcgaacacac caacgagctg gctgactaca tgctggacgc ccgcaccggg
    15901 tccatcatct ggtcccaccc gacctgcggc tgcgacaacg gccgcggtgt ggcgggcgat
    15961 atctacgccg ggagcgcggg ggccgaggcc tggtcctccg ccgtcgccgg actcagcaac
    16021 acctcgggag ggcaggcgtc ttcgcggaag ccgggctcca cgaacttcct gatctggtgg
    16081 gacaccgaca cgcttcgcga gctgctcgac agcaatcacg tcgacaagta cggcacgggc
    16141 ggcgacacgc gcctgctgac ggcggacggc accacgtcga acaacggcac gaagtcgacg
    16201 ccggcgctga gcggcgacct cttcggcgac tggcgcgaag agctggtgct gcgtacgact
    16261 ggcaacaacg cgctccgcat ctacaccacg accaacgtgg cgacgaaccg catctacacg
    16321 ctgctgcacg acccgcaata ccgcgtggcc atcgcgtggc agaacacggg gtacaaccag
    16381 cctccgcatc ccagcttctt catcggcaat ggcatgtcca cgccgccgac gccgaacatc
    16441 gtgctcgtgg gcggcgctcc gccggacccg cagcccggaa cgcccaccta ccagctggag
    16501 acggcttcat tgggcggtgg caccgtcagt gaaagcacca acggcggatt catgggcagc
    16561 ggctacgcga acttctcggc cacgggtggc ttcgcgcagg tgaacaatgc cgacggacgc
    16621 ggtggcggca gccatgcaat gcggattcgt tatgcgctcg gcgccaccgc gagccgtaca
    16681 ggccgcgtgc tggtgaacgg tgtcgcacag aacatcacgt tcaatcccac gggtggctgg
    16741 actacgtggg cgacacaaaa tgtgacgttc acgttcaaca acggcacggt gaacaccatc
    16801 cggttcgaat ccaacggcca agacctggcc aacgtcgacc agatcgagat cctgtaagaa
    16861 agtcggtgcc gggtggggaa agcggggagt gccactcccc actcctcacc cggcaccttt
    16921 cctcaatcgc ccgtgcgcgc gccccagagc gatacgccgt gctgggcctg cacactgaag
    16981 gtcaccacga atcggttggc gttggtattc cactggcgtg acaacacgcc gttgtagatc
    17041 acgccgctca gtgcgatgtc gatgagattg ttgccacggt gcagccacgt acccgtcatg
    17101 gcgccggtca tcgtgccgtc ggcattcaag cggatcgctt gtgaagtctt gatcgtggcg
    17161 ctgatgtcat caccgtgatc gatgagtttg taggcgcccg ccacgtcggc attggtgacg
    17221 tcggccccca cggcagtcgg gctcaaagac agcggtgcgt agcggaacgg cgccgccacc
    17281 ggccagcccg cggaattgaa catgagctgg tgcacgcgca gttcgtgctg ttcgccgcgc
    17341 gccggaaagc gggtgtggaa aatcaggaag tacttgtcga gcgccgcgtc gtagtacgcg
    17401 gagttgtggc ccggggatac atacccttcg gcggtgccgg tctcgccggt ggccagcgaa
    17461 aactggaaat tccccatgag tttctggccg tgcggggcaa ttcgagtgtc gtcgaacagc
    17521 accgcgggat tgccgcgcac cgtgtccatg ttcgtgcccg cggcatcgaa gtacggacca
    17581 tcgggcgcct gggaacgcgc gacgcggatg ttgtaaccgc cgttggcggc cagcccgcca
    17641 aacgaagtga acatgtaata gaagccggtc tgcgcgttgt agagcacgta cgcaccttcg
    17701 atgcgcgcgt ggttgccgcc taacaaatgt ctgccgtaac cctggccggg ctccggcagg
    17761 ccagtggtct cgtccatggc gagaatgaaa atgccgcccg aatacgaccc atagatcatc
    17821 cacaggtggc cggtggcgtc gaagaacgtc tgcgggtcca cgacgtttgg atgcacctgg
    17881 gcgtcgtagt tggtgacgcc gtcctcgctg aggccggcca tgcccgagcg caggatgatc
    17941 tgcctgttcg tgtacggacc gttgatcgaa tccgcgacgg cgacaccgag cgccgacagc
    18001 ggggagtcgc cgcggcagga gttgtaatag aagtagaact tgttgtcggc cagcttcacg
    18061 acatcgggcg cccacaggtc gttcacagtc gaccatgcga aggtctgctg gagcgccgtc
    18121 agcacattgg tgaacagtgg attggcgttg ttgacgccgt ccgccaccag cgtccagttc
    18181 atgagatccg tggtcctggc cgcggcgaga tgcgaaccga atacgtagta ctcagcgccg
    18241 accttgatga ccgacgggtc atgcactgaa gcgttgacga acgtgatggc cggcggcgga
    18301 gtcacgccac cggatgagga agaagaggac gaggaggacg agctggagct gccgctattg
    18361 ctcgatcctt ggccgccacc accacacgca gccagcgtca gaaaaccgag gaagccaaga
    18421 gcgcgaatcg tccgggtcat tttcatgccc ggaaaggtgc ctgagctggt gagcccatcc
    18481 aatgcccaaa aggctagcct gcgatatcaa ttggccggcg ggcgtttggt ctgggcgccc
    18541 aggaaatccc gtttgccgat gctcacgccg ttgtggcgca ggatggcgta ggccgtggcg
    18601 agatggaaaa agaagttcgg cacggagtgc tgcacgaaat agtccgcgcc agtcatccat
    18661 tcgcctttcc agcgcggctg gctgacgacg cgcgtggcgg cgccgtcgaa gtccgagg
//
LOCUS       DASQJS010000120        12934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2891753, whole genome shotgun sequence.
ACCESSION   DASQJS010000120 DASQJS010000000
VERSION     DASQJS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12934
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2891753"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VEW08_04925"
     CDS             complement(<1..833)
                     /locus_tag="VEW08_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ40110.1"
                     /translation="MTNPLTKNANGSPAPAGDAVIEVRDVRYAVGGRAIFDGLNLTAR
                     RGRITAFMGPSGTGKTTLLRLITGQATAERGSVRVFGDEVSTLSRKDLFALRQRMGML
                     FQNGALLTDEDVYENVAFPVRAHTNLPEPLIRQLVLTKLHAVGLRGAARLTPAELSGG
                     MQRRVALARAIVMDPEILIYDEPFVGLDPISMGVICRLIKQMNEALGITSIVVSHDVH
                     ELSTIADDSFLLSGGRVVAQGTPAELHKSDLEEVRQFMGGLADGPVAFHYPARDLAQD
                     LL"
     gene            973..1443
                     /locus_tag="VEW08_04930"
     CDS             973..1443
                     /locus_tag="VEW08_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HYJ40111.1"
                     /translation="MLRIGKLTDYATVILATLASDRSRLQNAGSLAERTHIAAPTVSK
                     LLKQLHRAGLVISTRGTHGGYQLAREPEAISAATILDALEGPLALTECSSATGHCGIE
                     HTCSVGRAWQRLNVAIRRSLSEVSLAQLAGMDSRSPTMPIERELKQLVRPKVPA"
     gene            1440..2882
                     /gene="sufB"
                     /locus_tag="VEW08_04935"
     CDS             1440..2882
                     /gene="sufB"
                     /locus_tag="VEW08_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HYJ40112.1"
                     /translation="MSTEPIDALLGKSYRHGFITDIDSDTLPPGLDEDVVRFISRKKR
                     EPAFMTEWRLKAYRHWLTMREPHWAKLRVNKIDYQSISYFSAPKRDPNAPKSLAEVDP
                     KLLATYEKLGVPLHERARLAGVAVDAVFDSVSVATTFKERLAEAGVIFCSFSDAVQNH
                     PALIEKYLGTVVPHTDNFFAGLNAAVFSDGSFVYVPPGVRCPMELSTYFRINAKNTGQ
                     FERTLIIAAPGSHVSYLEGCTAPMRDENQLHAAVVELIAEDDAEIKYSTVQNWYPGDE
                     NGLGGIYNFVTKRGECRGRNSKIAWTQVETGSAITWKYPSCVLRGENSVGEFYSVATT
                     NNYQQADTGTKMIHIGANTKSTIVSKGISAGHGQNSYRGLVKILPSAHGARNFTQCDS
                     LLMGPNCGAHTFPYIETRNPSATVEHEASTSKISEDQLFYCLQRGIEEEDAVNMIVNG
                     FCKSVLKELPMEFAVEAQNLLAVSLEGAVG"
     gene            2879..3643
                     /gene="sufC"
                     /locus_tag="VEW08_04940"
     CDS             2879..3643
                     /gene="sufC"
                     /locus_tag="VEW08_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HYJ40113.1"
                     /translation="MTAATPLLEISNLSAKVGGKNVLHGLSLTVNAGEVHAIMGPNGS
                     GKSTLAYVLAGRPGYEITGGGVKFQGRDLLTMEPEERARAGMFLGFQYPVEIPGVNNV
                     YLLKAAVNAKRKATGQPEVDAYEFLQLVRGKMKLMQMDESFLSRGVNEGFSGGEKKRN
                     EILQMLVLEPALAVLDETDSGLDIDALKVVARGVNSLRAADRGVLLITHYQRLLDHIV
                     PDQVHVLARGRIARSGDKSLAHELEKRGYDWVLEVA"
     gene            3643..4842
                     /locus_tag="VEW08_04945"
     CDS             3643..4842
                     /locus_tag="VEW08_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HYJ40114.1"
                     /translation="MIALPTNKDENWRYANLRPLAKAKADAVAAEAATHAAITLPAAL
                     PGYERWVFVDGRFAADLSAPAPDSCAALLNARDAGEDFSAMLDAAIATEGADLALARV
                     NGTRGDQVLHIAPPDGAEANIELTFIASAGAATGTSYPRVQVHAGRGAHLRIVERHLS
                     SGSSDSAINAAFDLALRADATIDHCRLQNFADAASAYDTLLAHVGERATYRLRTITLG
                     GLASRSTILVKLAGRAARCELTAGSIANGIQTHDVFAEIEHSNADTVTRELYRGIATD
                     RGKLGFNGKMIVRESAPGADSDQSLKTLLTGTGAEASIRPQLEIYTDKVRAKHGATTG
                     KLDEQMLFYLLSRGIDRRTAQTLLQWAFIEDVISRMDCAPLRVEIELLVAAQLNEVSA
                     LEGLLGP"
     gene            4839..6080
                     /locus_tag="VEW08_04950"
     CDS             4839..6080
                     /locus_tag="VEW08_04950"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HYJ40115.1"
                     /translation="MNADPAYTGYDAQRVWHDFPILARQIHGRPLAFLDSAASSQRPA
                     SVIAAVDDYEKLHHANVHRGVHLLSQEATQMFEHARERVREFVNAASTREVIFTRGTT
                     ESINLVAQSYARPRLQRGDEILLTGLEHHANIVPWQLVAGQTGAVVKAVPIDRRGVVD
                     LADFAAHVNERTRIVACAHVSNALGTVLPVREIIRLAHARGAVVLIDGAQAVPHQRVD
                     LQALDCDFYAFSAHKMYGPTGIGALIGRESLLEAMPPWQGGGDMILTVSFEQTTFNVL
                     PFKFEAGTPNISGAVGMAAAIDYIDSLGLDRIHAHEQRLLKLATRKLEAVPGLTIIGT
                     APDKAAVISFTLDGIHPHDLGTILDSEGVAVRTGHHCAMPVMEFFGVPATARASFACY
                     SRESDIDALVAALARAREVFG"
     gene            6080..6523
                     /locus_tag="VEW08_04955"
     CDS             6080..6523
                     /locus_tag="VEW08_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HYJ40116.1"
                     /translation="MDLKDLYRDVILDHNKTPRLFGRLEPADSSADGHNPLCGDRLHV
                     TLRLDGDRVSDLRFDGKGCAISVASASLMCEAVKGKRRSEITHLFNEVHEVLTKHDAP
                     VPDDLGKLAALSGVREFPVRVKCASLCWHTLNAALERSHEPVKTE"
     gene            6528..7079
                     /gene="sufT"
                     /locus_tag="VEW08_04960"
     CDS             6528..7079
                     /gene="sufT"
                     /locus_tag="VEW08_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HYJ40117.1"
                     /translation="MRTHDNEPFVLRRDVKAVMVPAGIDIELKSGQSGFITQALGGSF
                     TLYMEGNLFRLAGSDADAIGKELTKQPELPPNATEDDVLAVAWDQLRTCYDPEIPINI
                     VDLGLIYECTVSAHPDGGRMINVRLTLTAPGCGMGDVLVQDIKQKLEIIPTITAANVE
                     LVFDPPWNQSMMTEAARLQTGMF"
     gene            7086..7394
                     /locus_tag="VEW08_04965"
     CDS             7086..7394
                     /locus_tag="VEW08_04965"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ40118.1"
                     /translation="MSDWTDIGSIAELESSSRLESDVDGYRVRVAQVNGKLYAFEDRC
                     THDDSPFDDAPIEDSEIICPRHGARFCLLTGAALSPPAYEPLRIFEARENAGRVEVRV
                     "
     gene            7391..7909
                     /locus_tag="VEW08_04970"
     CDS             7391..7909
                     /locus_tag="VEW08_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYJ40119.1"
                     /translation="MNANKSKRLTLIRHANAEQDSEVRDFERPLSRKGQNEALEMARR
                     FQERGLVPDLILLSAATRTRETAETFAKVLGVAARLLQAEDALYLAEGDFILGAIRAV
                     GPRVGHLMVIGHNPGISAAAISLAPEAITNDLPTCGTLTMQVSCPTWNLIDRRCVRDS
                     ERDSPRKFFDFG"
     gene            complement(7906..9753)
                     /locus_tag="VEW08_04975"
     CDS             complement(7906..9753)
                     /locus_tag="VEW08_04975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYJ40120.1"
                     /translation="MTGIHTRRWFASIPITGRFYIMLAILVIAEVGLVAGCLHAMKLQ
                     TEASADLARVAAVQRSLDRALTLHGNISTELQNAATAPSTTPNSLVTALRAQLDLTWA
                     LPASAEVESITDSLRIPGAQYLESVEGYVRQGGGAGGATAWAFADLEPRRVALETGLR
                     DAMTRMRGVLQRVETRVLAEADRSGSVRLWLIGGALAAALVATLVFWAIARSLQRALH
                     DVGEVAQALAAGDMDNRCDTHGSDEVSGLARMINLLADTMQGMLTRLRSEASRSTFSN
                     QLVEALEMADSEAEADRVIARAMGAISDVHPMELLLADSSRAHLERACVHPKNGSPNC
                     TVESPYSCIAVRRGHPQIFPDAEALNACPKLLGRMGGSLSAACVPVNFMGRALGVLHV
                     AGAKGKPPNPEQIAQLTALGVHAGTRIGTLRAFERSQLQASTDSLTGLTNRRAAEEVL
                     SNLMTVDSAFAVVMADLDFFKRLNDTQGHEAGDRALKLFADTARAVVRDRDHVARWGG
                     EEFALLLPGANAERAAEVVGRLRATLAESQLLSGTPVFTASFGISDSTMARDRETLIR
                     LADEALYRSKQAGRDCCTVGDPQIMSLPGVRRERDQGAVVDITQVARSS"
     gene            complement(9893..>12934)
                     /gene="putA"
                     /locus_tag="VEW08_04980"
     CDS             complement(9893..>12934)
                     /gene="putA"
                     /locus_tag="VEW08_04980"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HYJ40121.1"
                     /translation="RLPETAARNVGARALALVQAVRAQKGSGGALDAFLREYSLASRE
                     GVILMCLAEALLRIPDGETADRLIADKIPSGAWDEHLGDSESMLVNASTWGLMLTGRV
                     VSLDRSEVGEARSWYSRLVGKLGEPVARAALKQAMRILGHQFVMGRDIESALSRAAGK
                     EERDYRYSFDMLGEAALTRADAEGYREKYAAAIASVGKAVETRESITARHSISIKLSA
                     LHPRYEFSQTARVMAELYPVIESLVQQARDAGIGVTLDAEEAERLELSMLLVGRLLES
                     DVTRGYAGFGLAVQAYQKRAYATIEWLMKRLRETERRITLRLVKGAYWDSEIKRAQER
                     GLAGYPVFTRKPNTDVSYLACARLLESAGERVYPQFATHNAHTIAHVAEVFGGDANRF
                     EFQRLHGMGAELYDSVVRGPWGRFPCRVYAPVGAHEDLLPYLVRRLLENGANTSFVNR
                     IVDASLPAEEVVADPVAEVDALERVAHPRIPLPVNLFAPERANSAGFNFADGQAVDGL
                     LRDMDRASQQPWSAAPIISGQTRPGKAQVLANPADTAEQIGTVISADAAAVNDAIAVA
                     LRAQEDWDASGGEKRAAVLERAATLFEEHTAALVVRCVREAGKTLPDAIGEVREAVDF
                     LRYYAVRARRDFSHESSLPGPTGERNLMRLRGKGVFGCISPWNFPLAIFTGQVAAALA
                     AGNTVVAKPAEQTPLTAAYATGLMLQAGVPPDALHFVPGDGAEVGGALTRDPRLAGVV
                     FTGSTDTARLIERSMAARPGAIGTLIAETGGLNVMLADSSALAEQLVLDVVQSGFNSA
                     GQRCSALRVLLVQEEIVPRVKTLLAGCMDELVLGDPARLDTDVGPVIDDEALSMLEKH
                     AAQVTKGASWSHRARPAANARGRFFAPLAVQIEALSDLKREVFGPVVHIVSYRARDLD
                     AVVAAVNGLGYGLTLGIHTRIDGLAQRIARTLRVGNVYINRNMIGAVVGVQPFGGMGL
                     SGTGPKAGGPHYLHRMATEQTITTNTAAIGGNAGLLSLAAR"
BASE COUNT         2337 a         4339 c         4103 g         2155 t
ORIGIN      
        1 agcaagtcct gcgcaagatc gcgtgcgggg tagtggaacg ccaccggccc gtcggcgagc
       61 ccgcccatga actggcgtac ttcttcgaga tcgctcttgt gaagttccgc gggcgtgccc
      121 tgcgccacga cacgtccgcc cgagagcagg aagctgtcgt cagcgatcgt cgataactcg
      181 tggacatcgt gtgagacaac gatgctggtg atgcccaatg cttcattcat ctgcttgatg
      241 agccggcaga tcacgcccat ggaaatggga tcgagaccca cgaacggttc gtcgtagatc
      301 aggatttcag gatccatgac gatggcccgc gcgagcgcca cgcgccgctg catgccgccg
      361 gagagttccg ccggcgtgag cctggcggcg ccccgcagcc cgacggcgtg caacttggtc
      421 aacaccagct ggcggatcag cggctcgggc aggttggtat gcgcgcggac cggaaacgcg
      481 acgttttcat acacgtcctc gtcggtgagc aaagccccgt tctggaacaa catgcccatc
      541 cgctggcgca gcgcgaacaa gtccttgcgc gacagggtcg agacttcgtc gccgaacact
      601 cgcacgctgc cgcgctcggc ggtcgcctgc ccggtgatga gccgcagcag agtggtcttg
      661 ccggtgccgg acggccccat gaacgcggtg atacgacccc ggcgcgcggt gaggttcagt
      721 ccgtcgaaaa tcgctcgccc gcccacggcg tagcgaacat cgcggacctc aattacggca
      781 tcgccggctg gcgccggcga tccgttcgca ttcttcgtca acggattcgt caagcgtcca
      841 cgtcgtccgg gctgcgggaa gggcgcgaag gatagcaaaa gggctcccgc ccggctttct
      901 gaagcattga atccgtacta aactggtccc acttagaacg ggccccgaaa ataggcctgg
      961 agtgactaaa ccatgcttcg gatcggcaaa ctcaccgact acgcgacggt catcctggcc
     1021 accctggcct ccgaccgctc gcgcctgcaa aacgccggca gcctggcaga acgcacccac
     1081 atcgcggcac ctaccgtcag caaactgctg aaacagttgc accgcgccgg cctggtgatt
     1141 tccacgcgcg gcacccacgg cggctaccag ctcgcccgcg agccggaagc catcagcgcc
     1201 gccaccatcc tcgacgcgct cgaggggcct ctcgcgctca ccgagtgctc ctccgccacc
     1261 ggccattgcg gcatcgagca cacctgctcg gtcggccggg cctggcagcg gctcaatgtg
     1321 gcaatccgcc gttcgctcag cgaagtctcg ctggcgcaac tcgccggcat ggacagccgg
     1381 tcgcccacga tgccgatcga acgcgagctc aagcagctgg tccgcccgaa ggtgccggca
     1441 tgagtaccga accaatcgac gcgctgctgg gcaagtcgta ccggcacggg ttcatcaccg
     1501 acatcgactc ggacaccctg ccgccgggac tcgatgagga cgtggtgcgc ttcatctcgc
     1561 gcaagaagcg cgaacctgcg ttcatgaccg agtggcgcct gaaggcctat cgccactggc
     1621 tgaccatgcg cgagccgcac tgggcaaagt tgcgcgtgaa caagatcgac tatcagtcca
     1681 tctcgtattt ctccgcgccg aaacgcgatc cgaatgcgcc gaagagtctc gcggaagtcg
     1741 atccgaagct gctcgccacg tatgagaagt taggcgtacc gctgcacgag cgcgcgcggc
     1801 tcgccggcgt cgcggtcgat gcggtgttcg acagcgtgtc ggtggccacc acgttcaagg
     1861 aacgcctggc cgaagccggc gtcatcttct gttcgttctc ggatgcggtg cagaaccacc
     1921 ctgccctcat cgagaaatat ctcggcacgg tggtgccgca caccgacaac ttcttcgcgg
     1981 gcctgaacgc cgcggtgttc tccgacggtt cattcgtcta cgtgccaccc ggcgtccgct
     2041 gtcccatgga gctgtcaacg tatttccgca tcaatgcgaa gaacaccggc cagttcgagc
     2101 ggaccttgat cattgccgcg cccggatcgc atgtgagtta cctcgagggc tgcacggcgc
     2161 ccatgcgcga tgagaatcag ctgcacgccg ccgtggtgga actcatcgcc gaagacgatg
     2221 ccgagatcaa atactccacg gtacagaact ggtatcccgg tgatgagaac ggtctcggcg
     2281 gcatctacaa cttcgtcacc aagcgtggcg aatgccgcgg ccgcaattcc aagattgcct
     2341 ggacccaggt cgaaaccggc tcggccatca cctggaagta tccgtcctgc gtgttgcgcg
     2401 gggagaattc cgtgggtgag ttctattccg tggccacgac gaacaactat cagcaggccg
     2461 acaccggcac caagatgatc cacatcggcg ccaacacgaa gagcacgatc gtctcgaagg
     2521 gcatatcggc cgggcacggt cagaacagtt accgcgggct ggtcaagatc ctgccgtccg
     2581 cgcatggtgc gcgcaatttc acgcagtgcg attcgttact gatgggtccc aactgcggcg
     2641 cgcatacgtt tccctacatc gaaacgcgca atcccagcgc gacggtggag cacgaggcgt
     2701 cgacctcgaa gatcagcgaa gaccagctct tctactgcct gcagcggggc atcgaagagg
     2761 aggacgcggt caacatgatc gtgaatggct tctgcaagag cgtgttgaag gaactaccga
     2821 tggaatttgc cgtggaggcg cagaacttgt tagccgtgag cctggaagga gccgtgggat
     2881 gaccgctgca actcctttgt tggaaatctc caacttgtcc gccaaggtcg gcggcaagaa
     2941 tgtattgcac ggattgtcgc tgacggtgaa cgcgggcgaa gtacacgcca tcatgggccc
     3001 gaacggctcg gggaaaagca ccctcgccta cgtgctggct ggccggccgg gttatgaaat
     3061 caccggtggc ggcgtgaaat tccagggccg cgacctgctg acgatggagc ccgaagagcg
     3121 ggcgcgcgcc ggcatgttcc tcgggttcca gtatccggtt gagatccccg gcgtcaacaa
     3181 tgtctatcta ttgaaggccg cggtgaacgc caagcgcaag gcgacgggcc agccagaggt
     3241 ggatgcctac gaattcctgc agctggtgcg cggcaagatg aagctcatgc agatggacga
     3301 gagcttcctg tcgcgcggcg tcaacgaagg attttcgggc ggcgaaaaga agcgcaacga
     3361 gatcctgcag atgctggtgc tcgagcccgc gcttgccgtg ctcgatgaaa ccgattccgg
     3421 gctcgacatc gacgcattga aagtggtcgc gcgcggcgtg aattccctgc gtgccgccga
     3481 ccgcggcgtg ctgttgatca cccactacca gcggctgctc gatcacatcg ttcccgacca
     3541 ggtgcacgtg ctggcgcgcg gcaggatcgc gcgctcgggc gacaagagcc tggcgcacga
     3601 gctcgagaaa cgcggctacg actgggtgct cgaggtcgcg tgatgatcgc gttgcctacc
     3661 aacaaggacg agaactggcg ctatgccaat ctgcgcccgc tcgccaaggc aaaagccgac
     3721 gcggtagccg cggaagcagc aacgcacgct gcgatcacgc tgccggccgc gctgccgggc
     3781 tacgaacgct gggtattcgt tgacggccgt ttcgcggcag atctgtcggc acccgcgccg
     3841 gactcctgcg cggccctgtt gaatgcgcgc gatgccggag aagacttctc ggccatgctc
     3901 gacgcagcca tcgccaccga aggcgcggat ctcgcgctgg cgcgtgtgaa cggcacgcgc
     3961 ggcgaccagg tgctgcacat cgccccgccc gatggtgcgg aggcgaacat cgaattgacg
     4021 ttcatcgcaa gcgcaggcgc cgccaccggc acctcctatc cgcgcgtcca ggtgcacgcg
     4081 ggccggggcg cgcacctgcg catcgtcgag cgccatttga gctcgggcag ctcggactcg
     4141 gcgatcaacg ccgcattcga tctggcgctg cgcgccgatg ccaccatcga ccactgccgc
     4201 ctgcagaatt tcgccgatgc cgcgagcgcg tacgacacct tgctggcgca cgtgggcgag
     4261 cgggcgacct atcggctgcg caccatcacg ctgggcgggc tcgcttcccg ttccacgatc
     4321 ctggtcaagc tcgccggccg cgccgcgcga tgcgaactca ccgcgggaag cattgccaac
     4381 ggcatccaga cccacgacgt gtttgccgag atcgaacact ccaacgccga caccgtgacg
     4441 cgtgagttgt atcgcggcat cgccactgat cgcggcaagc tcggcttcaa cggcaagatg
     4501 atcgtgcgcg agtccgcgcc cggcgccgat tccgaccagt cgctgaaaac cctgctcacc
     4561 gggaccggcg cggaagcttc aatccgcccg cagctcgaaa tctacaccga caaggtgcgc
     4621 gccaaacacg gcgccaccac cggcaagctc gacgaacaga tgctgttcta cctgctgtcg
     4681 cggggtatcg atcggcgtac cgcgcagacc ctgctgcaat gggcattcat cgaagacgtg
     4741 atatcgcgca tggactgtgc tccgctgcgc gttgaaatcg aactgttggt cgcggcgcag
     4801 ctcaacgaag tgtctgcact cgaaggttta ttagggccat gaacgcggat cccgcttaca
     4861 caggctacga cgcgcaacgc gtgtggcacg acttccccat cctcgcccgg cagatacacg
     4921 ggcggcctct cgcattcctc gacagcgccg cctcgtcgca gcgcccggcc agtgtcatcg
     4981 ccgcggtcga cgactacgaa aagctccatc acgccaacgt gcatcgtggc gtgcacctgc
     5041 tgagccagga agccacgcag atgttcgagc atgcgcgcga acgcgtgcgc gagttcgtga
     5101 atgccgcgtc cacgcgcgag gtcatcttca cgcgcggcac caccgaatcc atcaacctgg
     5161 tggcgcagtc ctacgcgcgg ccgcgcctgc agcgcggtga cgagatcctg ctcaccggcc
     5221 tcgaacacca cgccaacatc gtgccgtggc aactggtcgc cgggcagact ggcgccgtcg
     5281 tcaaggccgt gcccatcgat cggcgcggcg tcgtcgacct ggcggatttc gccgcccacg
     5341 tgaatgaacg cacgcgcatc gtggcctgcg cgcacgtctc caatgcgctc ggaaccgtgc
     5401 tgccggtgcg cgagatcatc cgtctcgcac acgcgcgcgg cgcagtggtg ctcatcgacg
     5461 gcgcccaggc cgtgccgcat cagcgcgtgg atctgcaagc gctcgactgt gatttctatg
     5521 cgttctcggc gcacaagatg tacgggccca cgggcatcgg cgccctgatc ggccgcgaat
     5581 cgctgctcga ggccatgccc ccgtggcagg gcggcggcga catgattctc accgtgagct
     5641 tcgagcagac cacgttcaac gtgctgccgt tcaagttcga ggccggcacc cccaacattt
     5701 ccggcgcggt gggcatggcg gcggccatcg actacatcga ctcgctgggg ctggaccgca
     5761 tccacgcgca cgaacagcgg ctgctgaagc tggcgacgcg caagctcgaa gcagttcctg
     5821 gcctgaccat catcggcacc gcgcctgaca aggccgcggt gatctcattc acgctggacg
     5881 gcattcatcc gcacgacctc ggcaccatcc tcgattcaga aggcgtggcg gtgcgcaccg
     5941 ggcaccattg cgccatgccg gtcatggaat tcttcggagt gcccgcgacg gcgcgcgcgt
     6001 cgttcgcctg ctattcgcgc gagtccgaca tcgacgcgct ggttgccgcg ctcgccaggg
     6061 cgcgcgaggt gttcggctga tggacctcaa ggatctctat cgcgacgtga tactcgatca
     6121 caacaagacc ccgcgactct tcggcaggct cgagcccgcc gactcctcgg ctgatggcca
     6181 caatcctctg tgcggcgacc ggctgcacgt gacgctgcgg ctcgatggcg accgtgtctc
     6241 cgacctgcgc ttcgacggca agggctgcgc catctccgtc gcctccgcgt cactgatgtg
     6301 cgaagccgtc aagggcaagc gccgttccga gatcacgcac ctgttcaacg aagtgcacga
     6361 ggttttgacg aagcacgacg cgccggtgcc tgatgacctg ggcaagctcg ccgcgctgtc
     6421 cggcgtgcgc gaattcccgg tgcgcgtgaa gtgcgccagc ctgtgctggc acacgctgaa
     6481 tgcggcgctg gagcgaagtc acgagcccgt gaagactgaa taatcagatg agaactcacg
     6541 acaacgaacc ctttgtcctt cgccgcgatg tcaaagcggt catggttccg gctggaatcg
     6601 atatcgagct caagtccgga cagtccggtt tcatcacgca ggcgctcggt ggcagcttca
     6661 cgttgtacat ggaaggcaac ctgttccggc tcgcgggcag cgatgccgac gcgataggca
     6721 aggaactcac gaagcagccc gagcttccac ccaatgccac cgaggacgac gtgctggcgg
     6781 tggcctggga tcagctgcgc acctgctacg acccggagat tccgatcaac atcgtggacc
     6841 ttgggcttat ttacgagtgc acggtgagcg cgcaccctga tggcggacgc atgatcaacg
     6901 tacggctcac cctgaccgca cccgggtgcg gaatgggcga cgtgctggtt caggacataa
     6961 agcagaaact ggagatcatc ccgacgatca ccgccgcaaa cgtggagctg gtgttcgatc
     7021 cgccgtggaa ccagtcaatg atgaccgagg ccgcgcgcct gcaaacaggg atgttctaat
     7081 aagtcatgtc cgactggact gacatcggca gcatcgccga actcgaatcc agttcacgtc
     7141 tcgagtccga tgtcgacggc taccgagtgc gggtagcgca ggttaatggg aagctgtacg
     7201 cgttcgaaga ccgctgtact catgacgact ccccctttga cgatgccccc attgaagact
     7261 ccgaaatcat ctgcccgcgt catggcgcgc gcttctgcct gctcaccggc gcggcacttt
     7321 ccccacctgc ctacgaaccc ctgcggatat tcgaagcacg cgaaaacgcc ggaagggtcg
     7381 aggtgcgcgt ttgaacgcta acaagtccaa acgcctcacc ctgatccggc atgccaatgc
     7441 ggagcaggat tccgaggtgc gcgacttcga gcgcccgctg agccgcaagg gccagaacga
     7501 agcgctggag atggcgcggc gctttcagga acgcggcctg gttccggatc tcattcttct
     7561 cagtgcggcc acgcgcacgc gtgaaaccgc cgagactttc gccaaagtgc tgggagtggc
     7621 cgcgcgcctg cttcaggccg aagatgcgct gtacctggcc gaaggcgact tcattctcgg
     7681 cgccattcgt gccgtcgggc ctcgcgtcgg acatctgatg gtcatcgggc acaaccccgg
     7741 gatctccgcc gcggcgatct cgctcgcacc cgaggccatc accaacgacc tgccgacttg
     7801 cggcacgctg acgatgcagg tgagctgccc gacctggaac ctcatcgacc gtcgctgtgt
     7861 gcgcgattcc gagcgcgact cgccgcgaaa gttcttcgac ttcggctaac tgcttctggc
     7921 gacctgggtg atgtcgacga ccgccccctg gtcgcgctcg cggcgcacgc ccggcaggct
     7981 catgatctgc ggatcgccga cggtgcagca atcgcggccg gcctgcttcg agcggtacag
     8041 ggcctcatct gcgaggcgaa tcagcgtttc gcgatcgcgg gccatggtcg agtcggagat
     8101 tccaaagctc gccgtgaaca ccggcgtgcc cgaaagcagt tgggactcgg ccagtgtcgc
     8161 gcgcaacctg cccacgactt ccgcggcacg ctcggcgttc gcgcccggca gcagcagcgc
     8221 gaattcctca ccgccccagc gcgccacgtg gtcgcggtct cgcaccacgg cgcgtgccgt
     8281 atcggcgaac agcttgagcg cccggtcacc ggcttcatga ccctgcgtat cgttcaggcg
     8341 tttgaaaaag tcgagatcgg ccatgacgac cgcgaacgcc gaatccacgg tcatgaggtt
     8401 gctgagcact tcctcggcgg cacgccggtt ggtgaggccc gtcaggctgt cggtgctcgc
     8461 ctgcagctgc gaacgttcga aggcgcgcag cgtgccgatg cgcgtaccgg catgaacgcc
     8521 gagcgccgtc agctgcgcga tctgctcggg attcggcggc ttgcccttgg cgccggccac
     8581 gtgcaacacg ccgagcgcac ggcccatgaa attcaccggc acgcaggcgg cggacagcga
     8641 accacccatc cggcctaaca acttcgggca ggcattcagc gcctcggcgt cggggaatat
     8701 ctgcggatgt ccacgccgca ccgcgatgca gctgtacggc gattccaccg tgcagttcgg
     8761 cgagccgttc ttgggatgca cgcaggcgcg ctccaggtga gcccgcgagg agtccgccag
     8821 caacagctcc atcgggtgta cgtcggagat ggcgcccatg gcgcgcgcga tcacgcgatc
     8881 cgcctcggcc tcggagtcgg ccatttccag cgcttccacc agctggttgc tgaacgtgct
     8941 gcggctggct tccgagcgca ggcgcgtcag catcccctgc atggtatcgg ccagcaggtt
     9001 gatcatgcgt gcgagcccgc tcacttcgtc ggagccgtgt gtgtcacagc ggttgtccat
     9061 gtcaccggcg gcaagcgcct gcgccacttc gccgacatcg tgcaacgcgc gctggaggga
     9121 acgcgcgatc gcccagaaca ccagcgttgc gaccagggcc gccgccagcg ccccaccgat
     9181 caaccacagc cgcacgctgc cggatcgatc cgcctcggcg agcacgcgcg tctcgacgcg
     9241 ctgcaggacg ccgcgcatgc gggtcattgc atcgcgcaat ccggtttcga gcgcgacgcg
     9301 gcgcggctcg aggtcggcaa atgcccacgc cgtcgcacca cccgcaccac caccctgacg
     9361 cacatagcct tcgacgctct cgaggtactg cgctccgggg atgcgcagag aatcggtgat
     9421 cgactcgact tcggcactcg ccggcagcgc ccaggtgaga tcgagttgcg cgcgcaatgc
     9481 agtgaccagg ctgttgggcg tggtgctcgg agccgtcgcg gcgttctgca gttcggtgct
     9541 gatatttcca tgcagcgtca gcgcgcgatc cagcgatcgc tggacggcgg caacgcgcgc
     9601 caggtcggcg ctggcttcgg tctgcagctt catggcgtgc aggcagccgg cgaccaggcc
     9661 cacctctgca atcaccagga tcgcaagcat tatgtagaag cgcccggtga tgggaatcga
     9721 tgcaaaccac cggcgcgtgt gtattcctgt catcgcagta cgcccctccc ggcgaatcgc
     9781 acagcagggt atcggcggcg ccggggtgga cctgaggaca gcgaaacgcg aaagacactg
     9841 cgccacgcga cgcagtgctc agtttccgcc acccggggcc gtgtctgacg tgttatcgcg
     9901 ctgcgagcga caacaggccc gcgttgccac cgatcgccgc ggtattggtg gtgatggtct
     9961 gttcggtcgc catgcggtgc aggtaatgcg gcccgccggc tttcggcccc gtgccggaga
    10021 gacccattcc gccgaagggc tgcacgccca ccaccgctcc aatcatgttg cgattgatgt
    10081 acacgttgcc gacgcgaagc gtgcgagcga tgcgctgcgc cagtccatcg atgcgcgtgt
    10141 ggatgccaag cgtgagcccg taccccagcc cattgacggc cgcgaccacc gcgtcgagat
    10201 cccgcgcgcg gtaactcacg atgtgcacca ccgggccgaa gacttcacgc ttgaggtcgc
    10261 tcaaagcctc gatctggacg gccagcggcg cgaagaagcg gccacgagca ttggcggccg
    10321 gccgcgcacg atggctccac gacgccccct tggtgacctg cgcggcgtgc ttctcgagca
    10381 tcgacaaggc ctcgtcgtcg atgaccgggc ccacgtccgt gtcgagacgc gccggatcgc
    10441 cgagcaccag ctcgtccatg catccggcta acaaagtttt tacgcgagga acgatttctt
    10501 cctgcaccag cagcacgcgt aacgcggagc atcgctgtcc ggcgctgttg aaccccgact
    10561 gcacgacgtc cagcacgagt tgctcggcga gcgccgaact gtcggcgagc atcacgttga
    10621 gaccgccggt ctcggcgatc agagtgccga tggcgccggg cctggccgcc atggagcgtt
    10681 cgatgaggcg ggcggtgtcg gtggaacccg tgaacaccac gccggcgagg cgcgggtcgc
    10741 gagtcagcgc gccgcccact tcagcgccgt ctcccgggac gaaatgcagc gcatcgggcg
    10801 gcactccggc ctgcagcatg agaccggtgg cgtaggccgc ggtcagcggt gtctgttcgg
    10861 ccggcttcgc gaccacggta ttgccggcgg ccagcgctgc cgcgacctgg cccgtgaaga
    10921 tagccagcgg aaaattccag gggctgatgc agccgaacac gcccttgccg cgcaggcgca
    10981 tcaggttgcg ttcgcccgtg ggacccggca gcgaggactc gtgcgagaaa tcgcggcgtg
    11041 cgcgcacggc gtagtagcgc aggaaatcga ccgcctcgcg cacttcgcca atggcgtcgg
    11101 gcagcgtctt gccggcctcg cgcacgcaac gcaccaccag ggccgcggtg tgttcttcga
    11161 aaagcgtcgc cgcgcgttcc agcacggccg cgcgtttttc tccaccggac gcatcccagt
    11221 cttcctgggc gcgcagcgca actgcgatgg cgtcattcac ggcggcagcg tccgcggaga
    11281 tcacggtgcc gatctgctcg gcggtatcgg cgggattggc gagcacctgt gctttgcccg
    11341 gccgcgtctg gccggagatg atcggcgccg cgctccaggg ttgttgggag gcgcgatcca
    11401 tgtcacgcag caacccatcc acggcctgcc cgtcggcgaa gttgaaaccc gcggaattcg
    11461 cacgctccgg tgcgaacagg ttcaccggca gcgggatgcg cggatgcgcc acgcgctcga
    11521 gcgcgtcgac ctcggccacc ggatcggcca caacctcttc agcaggcagg ctcgcatcga
    11581 cgatgcgatt gacgaacgaa gtattggcgc cgttctcgag caggcgccgc acgagatagg
    11641 gcaacaggtc ttcgtgcgcg cccaccggcg catacacgcg gcagggaaac cgtccccagg
    11701 gtccgcgcac cacgctgtca tacagttccg cgcccatgcc gtgcagacgc tggaattcga
    11761 atcgattggc atcgccgccg aagacttccg cgacgtgcgc gatggtgtgc gcattgtgcg
    11821 tggcgaattg cggataaaca cgttcgccgg cgctctccag cagccgcgca caggcgagat
    11881 aggaaacgtc ggtgttgggc ttgcgcgtga acaccgggta acccgcgagg ccacgctcct
    11941 gcgcgcgctt gatctcgctg tcccagtacg cacccttgac caggcgcagc gtgatgcgcc
    12001 tttcggtttc gcgcaggcgc ttcatcagcc actcgatggt ggcgtaagcg cgcttctggt
    12061 aggcctgcac ggcgagcccg aagcctgcgt agccgcgagt cacgtcgctt tcgagcaggc
    12121 ggccgactaa caacatggac aactcgaggc gctctgcctc ctcggcatcg agcgtgacgc
    12181 cgatgccggc atcgcgcgcc tgctgcacga gcgactcgat gacgggatag agctcggcca
    12241 tgactcgcgc ggtctgtgag aattcatacc gcggatgcag cgccgaaagc tttatggaga
    12301 tgctgtggcg cgcggtgatg gactcgcgcg tctccaccgc cttgccgacc gaagcaatcg
    12361 ccgccgcgta tttctcccga tagccctcgg catccgcacg cgtgagcgcg gcctcgccca
    12421 acatgtcgaa ggagtagcga tagtcgcgct cttccttgcc ggcggcgcgc gacagcgcgc
    12481 tttcgatgtc gcgacccatg acgaactggt ggccgagaat gcgcatcgcc tgcttgagcg
    12541 cggcgcgcgc cacgggctcg ccgagtttgc cgaccagccg cgaataccag gaccgcgcct
    12601 cgcccacttc gctacggtcc agcgacacaa cgcggccggt gagcatcaat ccccacgtgg
    12661 aggcattcac cagcatggat tcggagtcgc ccagatgttc atcccaggcg ccgctcggaa
    12721 tcttgtcggc gatgagccgg tccgcggttt cgccatcagg gatacgcagc agcgcttcgg
    12781 cgaggcacat gaggatcacg ccctcgcgcg acgccaggct gtattcgcgc aggaacgcgt
    12841 cgagcgcgcc gccactgccc ttctgcgcgc gcacggcctg caccagcgcc agtgcccgcg
    12901 ccccgacgtt gcgggcggcc gtctcgggca atcg
//
LOCUS       DASQJS010000121         5669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1318807, whole genome shotgun sequence.
ACCESSION   DASQJS010000121 DASQJS010000000
VERSION     DASQJS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318807"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VEW08_04985"
     CDS             <1..934
                     /locus_tag="VEW08_04985"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HYJ40122.1"
                     /translation="ATIDRWDPAMDAIVPADWKIEKLADGFGWAEGPIWVKNGGYLLF
                     TDVPGNKMWKWSEKGGLEKFLDPSGAANPDMNIWREAGANGLAILDKNSILLADTGSR
                     MVQRLDLMTKKKTPFASQFDGKKFSSPNDVVRMKSGVVFLTDPPYGFKKFDDAPEKEQ
                     PFNGVYRVANDGSVSVVEKDLHRPNGVALSPDERTLYVAQSEPTKAIIMAYSLDADGN
                     VTGKTLFQDFTDLVGEKSPGLPDGLTVAADGTIFATGPGGVLVLSKEGKRLGRINDGK
                     ATANCKFGDDGKTLYLTSHDMLARIRLNVTGAGF"
     gene            975..2291
                     /locus_tag="VEW08_04990"
     CDS             975..2291
                     /locus_tag="VEW08_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HYJ40123.1"
                     /translation="MELTYVFASIVLLVLLISWGKVQPFLAFLVASAVAGALLGLDID
                     KIPAVLDRGVGNLLGGLLGIVCIGAMFGKLVADSGAAQKIAMVLMRAFGEKNATWALM
                     CTGFLVGIPLFYNVGFVLLVPLVFSVVQQTKVPPIYLGIPMFASLSVTHGFLPPHPAP
                     TAMLPQFGADMATTLFYGIIVALPAMIIAGPIFAKLFRNVKSNPITLFMAPMRPESEL
                     PSAFNCFATALLPVGLIAMSLLDSLFHVIPEGILPTFRFFTSPLIVMLLSLAIATYSL
                     GLSRGMKMQSLMDGYGNSVKDIAVILLIIAGAGTLKEVFVATGVDKNISAALTDIAID
                     PLVLGWLIAGVIRVSLGSATVAGMTAAGLMAPVVASGGVDPNLMVLAVGAGSLMFSHV
                     NDSGFWMAKEYFNLSLKETLMSWTLMEGIVGIVGIIGVLALEAILY"
     gene            2434..4347
                     /locus_tag="VEW08_04995"
     CDS             2434..4347
                     /locus_tag="VEW08_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ40124.1"
                     /translation="MSAPPVTANRKVYPLRDALYAALITFATSVIGLSIIYLQARDAQ
                     VQAVRSEVLQLATTGAAQIDVELHERLVSSSQEGSPEHERALAPLVRMHRASKNVLFM
                     YTAIMRDGKIYWILDTARHYRVPGNEVPADPVMTPYHGSDPDLLRAFREQTAQADREP
                     MVEPDHTYLSAYAPIRDSSGRFVGLFGVDMVLDELDSRIASLKRALAVALFVVLILSG
                     VAGVVALRIRQFSAAIVKKLRAARARAEENAAAAESASRAKAVFLAMMSHEIRTPMNG
                     MLGVADLLRTMSPNPEQKKLLDILGSSGESLLRIINDILDFSKIEAERLELHPKPFDI
                     RGLLDELDTLLNTQARTRQVAVVIDADPQLPEALDGDRQRLSQILLNLGTNAVKFTDR
                     GEVRLVLRLLGVTDGKARIEFSMRDTGIGMSEEALGRLFTPFTQMADSRRHRGGTGLG
                     LVITQKLVTLMGGEIRVTSVPGRGSSFTFSLELPVAEAVASDITARVRRLESLSILVA
                     EDNAVNQTIIEAMLRQLGHRIRIVGTGTAALETLVRESFDLVLMDCNMPEMDGLEATR
                     QLRAGSAGRRTVDVPVIALTANAMDGDRERCLAAGMNDFLAKPVSIAALRAAIDRVAV
                     PGRETGKTQRAAS"
     gene            complement(4403..5389)
                     /gene="mgrA"
                     /locus_tag="VEW08_05000"
     CDS             complement(4403..5389)
                     /gene="mgrA"
                     /locus_tag="VEW08_05000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HYJ40125.1"
                     /translation="MTYLPSDTRYQSMKYNRVGRSGVRLPAVSLGLWHNFGDVDVFQN
                     GRTIARRAFDLGITHFDLANNYGPSPGSAEETFGALMRKDFHPYRDEMIISSKAGYLM
                     WPGPYGEWGSRKYLVASCDQSLSRMGLKYVDIFYSHRFDPETPLEETMGALDYIVRSG
                     RALYAGISSYNPEQTAKAAALLKALGTPCLIHQPSYSMFNRWIEDGLLAELDKQHMGC
                     IVFSPLAQGLLSSKYLNGIPEGSRAAKHTSLDPDAITPAKVEKVKKLNEIAKARRQSL
                     AQMAVAWTLRHKQVTSSLIGASKVAHVDEAVGALNNLEFSAAELAAIESALK"
     gene            complement(5402..>5669)
                     /locus_tag="VEW08_05005"
     CDS             complement(5402..>5669)
                     /locus_tag="VEW08_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40126.1"
                     /translation="ANSAGTDGAWGVYPFLTSGTLLVSDIENGIFLLKKNETLPPPAP
                     PVVTNPPPSSGGGGGGGGALDGSVLILLAGFAMLRARPKAQKAN"
BASE COUNT         1021 a         1763 c         1815 g         1070 t
ORIGIN      
        1 agccaccatc gatcgctggg accctgcgat ggacgccatc gtgcctgcgg actggaagat
       61 cgagaagctc gccgatggtt tcggctgggc cgaaggcccg atctgggtga agaacggcgg
      121 ctatctgttg ttcaccgacg tgcccggcaa caagatgtgg aagtggtcgg agaaaggcgg
      181 cctcgagaaa tttctcgacc cttcaggcgc ggccaatccc gacatgaaca tctggcgcga
      241 agccggtgcc aacggcctcg ccatcctcga caagaacagc atcctgctgg ccgacaccgg
      301 cagccgcatg gttcagcgcc tcgacctcat gacgaagaag aagacgcctt tcgcgtcgca
      361 gttcgacggc aagaaattct ccagcccgaa cgacgtggtg cgcatgaaat ccggagtggt
      421 gttcctcacc gacccgccat acggcttcaa gaaattcgac gacgcgccag agaaagagca
      481 gccgttcaac ggcgtgtatc gggtggccaa cgacggcagc gtcagcgtcg tcgagaagga
      541 tctgcatcgc ccgaatggcg tcgcgttgtc gcccgatgag cgcacgctgt acgtggcgca
      601 gtcggaaccg accaaggcca tcatcatggc gtactcgctc gacgccgatg gcaacgttac
      661 cggaaagacg ttgttccagg acttcacgga cctggtcggc gagaaatcac ccggacttcc
      721 cgatggcctg accgtggcgg ccgacggcac catctttgcc accggccccg gtggcgtgct
      781 ggtgctgtcg aaggaaggca aacgcctggg acgcatcaac gacggcaagg cgacggccaa
      841 ctgcaagttc ggcgatgacg gcaagacgct gtacctcacg tcgcacgaca tgctggcgcg
      901 catacggctg aacgtcaccg gagccggatt ctaagaacgc ggttcaccgc gctggcagat
      961 agagggggtc tttcgtggaa ctcacgtacg tattcgcgtc gatcgtgttg ttggtgctgc
     1021 tcatcagctg gggcaaggtg cagccattcc tggcgttcct ggtggcgtcg gccgtggccg
     1081 gcgcgctgct cggcctcgac atcgacaaaa tccccgcggt tctcgatcgc ggcgtcggaa
     1141 acctgctcgg cggcctgctc ggcatcgtct gcatcggcgc catgttcggc aagctggtag
     1201 ccgacagcgg cgcggcgcag aagatcgcca tggtgctgat gcgcgccttc ggcgagaaga
     1261 atgctacctg ggcgctgatg tgcaccggct tcctggtggg cataccgctg ttctacaacg
     1321 tgggattcgt cttgctcgtg ccgctggtgt tctcggtggt tcagcagacc aaagtgccac
     1381 ctatctatct cggcataccg atgttcgcat cgctgtcggt gacacacggc tttcttccgc
     1441 cgcacccggc gcccaccgcc atgctgccgc agttcggcgc cgacatggcc accaccctgt
     1501 tctacggcat catcgttgca ttgccggcca tgatcatcgc gggtccaatt ttcgccaagc
     1561 tgttccgcaa cgtgaagtcc aaccccatca cgttgttcat ggcgccgatg cggccggaga
     1621 gcgaactgcc cagcgcgttc aattgtttcg ccaccgcact gctgcctgtg gggctgatcg
     1681 cgatgtcgct gctcgacagc ctgtttcacg tgatccccga gggcatcctg cccacgttcc
     1741 ggttcttcac cagcccgctg atcgtcatgc tgttgtcgct ggccattgcg acttactcgc
     1801 tgggcctgtc gcgcggcatg aagatgcagt cgctgatgga cggctatggc aactcggtga
     1861 aggacatcgc ggtgatcctg ctgatcatcg cgggcgctgg cactctcaag gaagtgttcg
     1921 tggccaccgg tgtcgacaag aacatctcgg cggcgctcac cgatatcgcg atcgatccgt
     1981 tggtcctggg atggctgatc gccggcgtga tccgcgtttc gctgggctcg gccaccgtcg
     2041 ccggcatgac cgccgcgggt ctcatggccc cggtggttgc ctcaggcggc gtcgacccga
     2101 atctcatggt gctcgccgtc ggcgccggta gtttgatgtt ctcgcacgtc aatgactccg
     2161 gcttctggat ggccaaggag tacttcaacc tgtcgttgaa ggaaacgctc atgagctgga
     2221 cgctcatgga aggcatcgtc ggcatcgtgg gcatcatcgg agtgctcgcg ctcgaggcga
     2281 tcctctattg attcaaggca gtttgtcggg tactagaaac ccctagctga aatctccgct
     2341 tcgtgcccgg gcgtgcaatt ccgtgccgcc cgaggctgcc ctgctgcgac ggcgctcacc
     2401 gagaaatcga ggggtagcgg gcatgattgg tggatgtccg cccctcccgt caccgccaat
     2461 agaaaggtgt atccgctgcg cgatgcgttg tacgcggcgc tcatcacctt tgcgacgtcg
     2521 gtcatcgggc tgtcgatcat ctatttgcag gcgcgcgatg cgcaggtgca ggccgtgcgc
     2581 agcgaagtgc tgcagctcgc gaccacgggc gcggcacaga tcgacgtcga actgcacgag
     2641 cgactcgttt cgtcctcgca ggaaggcagt ccggagcacg agcgagcgct ggctccgctg
     2701 gtccggatgc accgcgcgtc gaaaaacgtg ttgttcatgt acacggcgat catgcgcgac
     2761 gggaagatct attggattct cgacaccgcg cggcactatc gcgtgccggg aaacgaggta
     2821 cccgccgatc cggtcatgac gccttaccac ggaagcgacc cggatctgtt gcgcgcattt
     2881 cgcgagcaga cggcacaagc ggaccgcgag ccgatggtcg agcccgatca cacatatctc
     2941 agcgcgtatg cgccgatacg cgacagcagc ggccggtttg tcggtctctt cggcgtcgac
     3001 atggtgctcg acgagctcga ctcgcgcatt gcttcgctga agcgggcctt ggccgtggcg
     3061 ctgttcgtgg tgctgatcct atccggtgtc gcaggcgtcg tggcgctgcg catccgccag
     3121 ttcagcgcgg ccatcgtgaa aaaactgcgc gccgcgcggg cccgcgccga ggagaacgcg
     3181 gcggcggcgg aatccgcgag ccgcgccaaa gcggtgtttc tcgcgatgat gagccacgag
     3241 atccgcacac ccatgaacgg catgctgggc gtggccgatc tgctgcggac catgtcaccg
     3301 aatcccgaac agaaaaagct gctcgacatc ctcggcagca gcggcgagtc cctgctgcgg
     3361 atcatcaacg acattctcga cttctcgaag atcgaagcgg agcggctcga gctgcacccg
     3421 aagccgttcg atattcgcgg cctgctcgat gagctggaca cgttgttgaa tacgcaggca
     3481 cgcacgcggc aggtggcggt cgtcatcgac gcggatccgc aattgcccga ggcgctcgac
     3541 ggtgaccgcc agcggctctc gcagatcctt ctgaatctcg gcaccaacgc cgtgaagttc
     3601 acggaccgcg gcgaagtgcg gctggtcctg cggttgttgg gagtgactga cggaaaggcg
     3661 cgcatcgagt tttcgatgcg tgacactggc atcggcatga gcgaggaagc gctcggacgc
     3721 ctgttcacgc cgttcacgca aatggcggat tcccggcggc atcgtggcgg caccgggctc
     3781 ggcctggtga tcacgcagaa gctcgtgacc ctcatgggtg gcgagatccg tgtgaccagc
     3841 gtgccgggca gaggctcatc gttcacgttc agcctcgaac taccggtggc tgaagctgtc
     3901 gcctcggaca tcacggcgag agtgagacgg ctggaaagcc tttcgatcct cgtggccgag
     3961 gacaacgccg tgaatcagac catcatcgaa gcgatgctcc ggcagctcgg ccatcgcatc
     4021 cggattgtcg gtaccggcac cgccgcgctc gagacgctgg tgcgtgaatc gttcgacctc
     4081 gtgctcatgg actgcaacat gccggaaatg gacggactcg aggcgacgcg gcagctgcgt
     4141 gcgggatcgg ccggcaggcg taccgtcgac gtgccggtga ttgcgttgac cgcgaatgcc
     4201 atggatggcg accgcgagcg ctgcctcgcc gcaggaatga atgacttcct cgccaagccc
     4261 gtgtccatcg ccgccctgcg ggccgcgatc gatcgcgtgg cggtgccggg ccgggaaacc
     4321 gggaaaactc agcgagccgc gagctagggt tcggcttcgt ggaagcctgg caccgtttcc
     4381 taggaaacgg tgcctgacac cattacttca gggctgactc gatagcggcg agctcggcgg
     4441 ccgagaactc gaggttgttc agcgcgccga cggcctcgtc cacatgcgcc accttgctgg
     4501 cgccgatcaa cgaagacgtc acctgcttgt gacgcagcgt ccaggccacg gccatctgcg
     4561 ccagcgactg gcggcgggct ttcgcgatct cgttgagctt cttcaccttc tcgactttgg
     4621 ccggggtgat ggcgtccgga tcgagcgagg tgtgcttcgc ggcgcgtgaa ccttccggaa
     4681 taccgttcag atatttgctg ctcagcagac cctgtgccag tggcgaaaac acgatacaac
     4741 ccatgtgctg tttgtcgagc tcagccagca ggccatcttc gatccagcgg ttgaacatgc
     4801 tgtagctggg ctggtgaatg aggcaaggcg tcccgagggc tttcaacaac gcagccgcct
     4861 tcgcggtctg ctccgggttg tagctcgaga tgcccgcgta caacgcccgg ccggaacgca
     4921 cgatgtaatc gagcgcgccc atggtctctt cgagcggcgt ttccgggtcg aagcggtggc
     4981 tgtagaagat gtcgacgtac ttcaggccca tgcgcgacag cgactggtcg cagctcgcca
     5041 ccaggtattt gcgcgagccc cactcgccat acggacccgg ccacatcagg tagccggctt
     5101 tcgacgagat gatcatctcg tcgcgatacg gatggaaatc cttgcgcatg agcgcgccga
     5161 aggtttcttc cgccgatccc ggcgacggcc cgtagttgtt agccaggtcg aagtgggtga
     5221 tgccgaggtc gaatgcccgg cgtgcaatgg tgcggccgtt ctggaacaca tcgacgtcgc
     5281 cgaagttgtg ccacagaccg agcgagaccg caggcaggcg cacgccgctg cgtcccacgc
     5341 ggttgtactt catcgactgg taacgcgtgt cggacggcag ataggtcatg tagaggtctc
     5401 gtcagtttgc tttttgtgct tttgggcggg cgcgtaacat cgcgaacccc gccaacaaaa
     5461 tcaagaccga accgtcgaga gcgccaccgc ctccaccacc gccgcccgaa gagggcggcg
     5521 ggttggtgac caccggcgga gctggcggcg gcagggtttc attcttcttc agcaggaaaa
     5581 tgccgttctc gatgtcgctc accagcagcg tgccggacgt caggaacgga tagacgcccc
     5641 atgcgccgtc ggtgcccgcg gaattggcc
//
LOCUS       DASQJS010000122         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1633986, whole genome shotgun sequence.
ACCESSION   DASQJS010000122 DASQJS010000000
VERSION     DASQJS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633986"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VEW08_05010"
     CDS             <1..471
                     /locus_tag="VEW08_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HYJ40127.1"
                     /translation="HEQSHDPSRDHGRHADMNMRSAYVHVLADAATSVLAIVALLGGL
                     LWGAAWLDPVMGLVGAGLVAVWAFGLLRDSGRILLDAEMDAPVVAEVREVIEGGAVPA
                     TITDLHVWRVGRAKYAVVLSITTTSSEDADYFRRALSVHEELAHVTVEVSKSPA"
     gene            complement(513..659)
                     /locus_tag="VEW08_05015"
     CDS             complement(513..659)
                     /locus_tag="VEW08_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40128.1"
                     /translation="MLLRGHIDDFLQMLAPHLTQRQVQAAENANTQEGVDREFATVKI
                     PFLR"
     gene            complement(730..906)
                     /locus_tag="VEW08_05020"
     CDS             complement(730..906)
                     /locus_tag="VEW08_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYJ40129.1"
                     /translation="MKLIQLRCLVAVAGNDLNITQAARVLHAAQPGVRKQLKQLEDEL
                     GFQLFERKRLPCFC"
     gene            complement(1056..2075)
                     /locus_tag="VEW08_05025"
     CDS             complement(1056..2075)
                     /locus_tag="VEW08_05025"
                     /inference="COORDINATES: protein motif:HMM:NF008106.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYJ40130.1"
                     /translation="MEQTNWKRRLLIALTTSVFIGGAGTAAQAAEVTLLNVSYDPTRE
                     LYTDFNKSFAEYWKKKTGDTVKINQSHGGSGRQARSVIDGLAADVVTLALAADVDAIA
                     TNGKLLPVNWEERLPDHSAPYTSTIVFLVRKGNPKNIRDWGDLVRPGIGVITPNPKTS
                     GGARWNYLAAWAWAKKQPGGSDGTAKEFVRKLFRNVPVLDTGARGSTTTFAQRGIGDV
                     AISWENEAWLATKELGPDKFEIVIPSISILAEPSVAVVDKVVLRRGTRAIAEEYLKYL
                     YSPEGQEIAARNYYRPRNADVARKHAELFPRLELVTIKDFGGWGKAQKEHFGDGGVFD
                     QIYAQ"
     gene            complement(2113..3549)
                     /locus_tag="VEW08_05030"
     CDS             complement(2113..3549)
                     /locus_tag="VEW08_05030"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HYJ40131.1"
                     /translation="MKIRRAAVGASIAALLPFAGTALAQNAQPSDGNAELRREIEDQK
                     QRLLVLERKLEIQQEAATAAAATAPRITASASRFQIGSTDNSNFIRLRGTLHADARTY
                     GGDSVPEIADTFILRRVRPTIEGTFGGIYDFKFMPDFAGGRSVIVDAYTAARFKPGAI
                     VTVGKFKPPVGLERLQSSADIRFIERALPTNLVPNRDLGIQLSGDFSGGAFSYAVGYF
                     NGVTDGQSSDNLTTPDIEVDTAGDYAARLFFQPFANSSNFNLRGLGFGVGTTWVDVDG
                     TAANTALSAYRSPGQQSVFAYRANTATGVTPNNATFADGQRLRLAPQFHYYRGSFGLL
                     GEYTQVEQDVSRAVGGVTRSDTLTNSAWQAQFSWFLTGEEEAFRGFTPGSTFQPGKNG
                     TGAWELVARYHELDIDDDAFAGGATSFANPLTAVSKATAYGVGVNWYPWNTVKLSLNY
                     EQTEFEGGAATGDRADEQALLSRFAINF"
BASE COUNT          582 a         1104 c         1185 g          711 t
ORIGIN      
        1 cacgagcagt cacacgaccc ttcacgtgat catggccggc acgcggacat gaacatgcga
       61 tctgcatatg tgcatgtgct cgccgacgcc gccacatcgg tgctcgccat cgtggccttg
      121 ctcggcggct tgctgtgggg agccgcatgg ctggacccgg tcatgggtct cgtgggcgcg
      181 gggctggtgg cagtctgggc ttttggactg ctgcgcgact cgggacggat cctgctcgat
      241 gcggaaatgg acgcgccggt agttgcagag gtgcgcgagg tcatcgaggg tggggcagtc
      301 ccggcgacga tcaccgactt gcacgtgtgg agggtcggcc gcgcgaagta tgcggtggtg
      361 ctgagcatca ccaccacatc gagcgaggat gcggactact tccggcgcgc gctctccgtg
      421 cacgaggagc tcgcgcatgt gacggtcgaa gtcagcaagt cgcccgcctg accgcgtcga
      481 ctccccggaa tccccacccg gcaccttcct gcctagcgga ggaaaggaat cttcaccgtc
      541 gcgaattcgc gatccacgcc ttcctgtgtg ttcgcatttt cggcggcctg cacctgccgc
      601 tgagtgagat gcggcgccag catctgcaga aagtcgtcga tatgacctcg cagaagcacg
      661 ccgcgccggc atggtcggtt tgctgatctc gccgagcgcg tgtccgtcgg gcacgacgat
      721 ggcgcgatgt cagcagaagc agggcaggcg cttgcgttcg aacaactgaa agccgagttc
      781 gtcctcgagc tgcttcagct gttttctgac tccgggctgc gccgcgtgca gtacgcgcgc
      841 tgcctgcgtg atgttgaggt cgttcccggc taccgcgacg aggcagcgca actgaatcaa
      901 tttcatcttt ggtggtgggc catcggaggc ggtgagggtg aaaacataga gagggggtac
      961 tcttttctca gctcactcac ggcccccgcg gcccgtggaa agagtacggc gccaggggcg
     1021 ccgtacctac taactattga ttctttgcct atgcattact gcgcgtagat ctggtcgaac
     1081 actccgccgt cgccgaagtg ctccttctgc gccttgcccc agccgccgaa gtccttgatg
     1141 gtcacgagtt cgagcctggg gaaaagctcg gcgtgcttcc tggccacgtc ggcgttgcgc
     1201 ggacgatagt agttgcgcgc ggcgatctcc tggccttccg gcgagtacaa gtacttgagg
     1261 tattcctcgg cgatcgcgcg cgtgccacgg cgcagcacca ccttgtcgac cacggccacc
     1321 gagggctcgg caaggatgct gatcgacgga atcacgatct cgaacttgtc ggggcccagt
     1381 tccttggtgg cgagccaggc ttcgttttcc caggagatgg ccacatcgcc gataccgcgc
     1441 tgggcaaacg tcgtcgtgga gccacgcgca ccggtgtcga gaacgggcac gttcctgaac
     1501 agcttgcgca cgaattcttt cgcggtcccg tcgctgccgc ccggctgctt cttcgcccag
     1561 gcccaggccg cgagatagtt ccagcgcgcg ccgcccgagg tcttcggatt cggcgtgatc
     1621 acgccgatgc ccggccgcac caggtcgccc cagtccctga tgttcttcgg attgcccttg
     1681 cgaaccagga aaacgatcgt ggacgtgtag ggagcgctgt gatcaggcag gcgttcttcc
     1741 cagttcaccg ggaggagttt gccgttggtg gcgatcgcgt ccacgtcggc ggcgagcgcc
     1801 agcgtgacca cgtcggccgc gagtccgtcg atgaccgagc gcgcctggcg gcccgagccg
     1861 ccgtgcgact ggttgatctt gaccgtatca ccggtcttct tcttccagta ctcggcaaac
     1921 gacttgttga agtcggtgta caactcgcgt gtcgggtcgt atgagacgtt gagcagcgtg
     1981 acttcggcgg cctgcgctgc ggtgccggcg ccgccgatga aaactgacgt cgtcagcgcg
     2041 atcagcagtc ggcgtttcca gtttgtctgt tccatttgat tattgctcca atgccaatgt
     2101 cttgatgaat gatcagaagt tgatcgcgaa gcgcgagagc aacgcctgct cgtcggcgcg
     2161 gtcgccggtc gcggcgccgc cttcgaattc ggtctgttcg tagttgagcg acagcttcac
     2221 ggtgttccac gggtaccaat tgacacccac gccataggcg gtggccttgc tgaccgcggt
     2281 cagtggattc gcaaacgagg tcgcgccgcc cgcgaaggca tcgtcatcga tgtcgagctc
     2341 gtggtagcgc gcgaccagtt cccaggcgcc cgtgccgttc ttgccgggct ggaatgtgct
     2401 gcccggcgtg aagccacgga aggcttcctc ttcaccggtc aggaaccacg agaactgggc
     2461 ctgccatgcg gagttcgtca gcgtgtcgga gcgggtgacg ccgcccacgg cgcgcgagac
     2521 atcctgttcg acctgcgtgt attcgcctaa caagccgaag ctgccacggt agtaatggaa
     2581 ctgcggcgcg agacgcagac gctggccgtc ggcgaacgtg gcgttgttgg gcgtgacgcc
     2641 ggtcgcggtg ttggcgcgat aggcgaacac gctctgctga ccgggcgagc ggtacgccga
     2701 cagcgcggtg ttggcggcag tgccgtctac atcgacccag gtcgtgccga cgccaaatcc
     2761 caggccgcgc agattgaagt tgctggagtt cgcgaacggc tggaagaaca ggcgcgcggc
     2821 gtaatcgccg gcggtgtcca cttcgatgtc gggcgtggtg aggttgtcgc tgctctgccc
     2881 gtcggtcacg ccgttgaagt aacccacggc gtaactgaat gcgccgccgc tgaagtcacc
     2941 cgagagctgt atgccaaggt cgcgattcgg aaccaggttg gtcggcagcg cgcgctcgat
     3001 gaagcggatg tcggctgagc tttgcaggcg ctcgagccct acgggcggct tgaatttgcc
     3061 gacggtgaca atggcaccgg gcttgaatcg tgcggccgta tatgcgtcga cgatcaccga
     3121 ccggccgccc gcgaagtccg gcatgaactt gaagtcgtag attccgccga aggtgccttc
     3181 gatcgtcggc cgtacgcgcc gcaggatgaa ggtgtcggcg atttcgggaa ccgagtcgcc
     3241 gccgtacgtg cgtgcgtcgg cgtgcaacgt gccgcgcagt cgaatgaagt tcgagttgtc
     3301 ggtcgatccg atctggaagc gcgatgcgct ggccgtgatc cggggcgcag tcgccgcagc
     3361 ggccgtggcc gcttcctgct ggatctcgag cttacgttcg agcaccagca gcctctgctt
     3421 ctggtcctcg atctcgcgac gcaactccgc gttgccgtca ctcggctggg cgttctgcgc
     3481 cagcgccgtc cccgcaaatg gcagcagtgc cgcgatggac gcacccactg ctgctcgtct
     3541 gattttcacc ttcgttctcc tggattcgtg tgtgccacaa gg
//
LOCUS       DASQJS010000123        22934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_66024, whole genome shotgun sequence.
ACCESSION   DASQJS010000123 DASQJS010000000
VERSION     DASQJS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 22934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22934
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66024"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VEW08_05035"
     CDS             <1..1001
                     /locus_tag="VEW08_05035"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYJ40132.1"
                     /translation="PADPIVDPFADVDRAASAAFTAQGISGMGLAIYDANGVKRFEKM
                     YGTFSADQRVAIASSSKMVAGLVLMRLIDQGFLTLDSTTGTVLGWTGPQAAITLRQLL
                     SFTSGLEREAPCTLLAGITLADCVAAISQQAMVAAPGTRFDYGSTHLHVAARMAEVAT
                     GATWANLFATQLKTPLGLTSPDLAWYTAPRQAVGISNPLVAGGIRATMNEYARFLALD
                     FNRGVYQGNRLIGDALFTAQSTEPYPNAVIGNSPFVNAGINFHYGLAAWLECVPPATN
                     CAVLSSPGAFGFTPWFDRDGGYYAILGMEVTESQSGVVAFAVNLAQELKPLIRSAL"
     gene            complement(1037..1579)
                     /locus_tag="VEW08_05040"
     CDS             complement(1037..1579)
                     /locus_tag="VEW08_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40133.1"
                     /translation="MKMPRTLLVGFSGAMFALIAAPAMPQAPAGMGSSASISVATGPH
                     AGRYAFAPREACAIAAFSDQPLGLSVVLSSENSSLSLDMPNIDEKHAHEIQIVLVVAD
                     KRAMAGGKGMSSTTYEIDTRPDSALKPFQKAERANKGITGKARTTLMTQGNNVLLSFS
                     GETATGVKLEGEVTCRKVAA"
     gene            complement(1576..2319)
                     /locus_tag="VEW08_05045"
     CDS             complement(1576..2319)
                     /locus_tag="VEW08_05045"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HYJ40134.1"
                     /translation="MGFKFSSIVLCAALIAPAAQAQWTGKAELGYLQSSGNAESTSAN
                     TKFDLKRESEKWINAFFVSALYGNNGEFTNAERYEASYQLDYKINDRLSWFGALRGEK
                     DKFSGFVYQATVSTGAAYKFIDTPVTKLSFSLGAGYRQLQEEILIRTDAGEVIDRIEG
                     EEESDPVVTFASGYEHNFTESTKITNKFLAESGSENTALQNDLALQVSMTKTLALAVG
                     YGIRYNTDPPPLSETTDTLITVNLVYNIQ"
     gene            complement(2436..3071)
                     /locus_tag="VEW08_05050"
     CDS             complement(2436..3071)
                     /locus_tag="VEW08_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HYJ40135.1"
                     /translation="MKPTKLLAVLALMLPVMAHGQGFYAGLDVGEARSDAKTSENVLS
                     ATTARSSGSSTGFRLRGGYQFGRFVAIEAAYVDLGEIESHFDPDDCPVGAPGSCPLDV
                     RTSSSGLLVSVVGVLPIGEHWYLDARLGWGKIDFELDEIGGDGLEADEENTAFIYGIG
                     GGYRFNEHWGVVLDYSEYNQEDFLDSALEGDFGVYNLGETSVTSLGVSYRW"
     gene            complement(3098..3757)
                     /locus_tag="VEW08_05055"
     CDS             complement(3098..3757)
                     /locus_tag="VEW08_05055"
                     /inference="COORDINATES: protein motif:HMM:NF036878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYJ40136.1"
                     /translation="MATNYVLVDFENVQPESLAALANGPFRVKVFVGAAQAKGRISFE
                     LSHSMQMLGASAEYVRIARTGKNAVDMHIAYYVGRLLEKEPGAMIHIVSKDTDFDPLI
                     EYLRAKGSSCKRVKTITEVPKHATPRPAAPVSKVAKPTPHVPSPRRPHAEKLAPIIKH
                     LHSLSGKPATSKKLTQTIASYFKQHGGTLPDKTVALIIDELIRLKYVSQDGTKVSYHL
                     S"
     gene            4123..5502
                     /locus_tag="VEW08_05060"
     CDS             4123..5502
                     /locus_tag="VEW08_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40137.1"
                     /translation="MFLPCLARALAYLLSIGFLLGTARAATPVADGGREILGINDGWG
                     AVPTASLPMGTTGGSAAAPARTVAVTNRKELVAALAWPDPSPKLIYIKGVIDVNVDDA
                     GKVLTCKDYARPDPATGEMFSIYAFMAMYDPEGPHRKNNPWGGQEEARMASAAAQEAR
                     VRIRVPPNTTLYGVGGDATLIGAWLDIRGNTGSQPMNVIVRNISFQDTADCFPEWSPT
                     DGFTGNWNAAYDSISIAHATHVWIDHNRFADLRTRDDTQPVYFGRPLQVHDGHLDITD
                     ESDFVTVSWNQFTSHDKTMLIGNSDSATEDREHLRVTLHHNLFDGTGQRMPRVRFGKV
                     HVYNNVYRADRNTNYRSSWGAGTESQIYAENNYFEMSKTFGPQEVIDGKKGTRMTVTG
                     NCWTDEESCVPTDFLALHNAQFEPDIQPDAGWTPTLYGPAKAAEPAKSAQERVLSESG
                     PGRAYNNKN"
     gene            complement(5654..6016)
                     /locus_tag="VEW08_05065"
     CDS             complement(5654..6016)
                     /locus_tag="VEW08_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HYJ40138.1"
                     /translation="MTTSNPHGLTQLRDALREFAAERDWDQYHSPKNLASALAVEAAE
                     LLERFQWLTEDQSRALPPAELQKVREEMADVLNYLVRLADKLDVNLLEAAREKMKLNA
                     QKYPADKARGSAKKYSEL"
     gene            6092..10171
                     /locus_tag="VEW08_05070"
     CDS             6092..10171
                     /locus_tag="VEW08_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HYJ40139.1"
                     /translation="MRSPLWLLTTVLAVFSLPAGAAEAWDAPAFSVPARDLLQAATAV
                     KRERATAVVVLLDERTYVLDEQHRLTSISRLIYRVDSPDGVERWAASTAHWQPWHQAK
                     PTIRARVITLDGQEHQIDQKLLTDAANRSEDNQVYDDSHTLEGPLPAVAIGAVIEEEI
                     TVRDEKPFFSAGSVYREYIGRPMPVLRTRIVIDAPESLPLKRHMQLLPRAVVQEVRAN
                     GRVTWTLEHGVLDEMGEMDSNLPPDAPSWPTVEFTSATSWEAVAAAYRGMTEARIRND
                     DAKPLIAGLKATKSPSREYVAKVVERLHREVRYTGVEFADARLVPEYPAETLRRRFGD
                     CKDKSTLLVAALRASGIDAYLALLSSGDDQDVSPDLPGLGMFDHAIVYVPGVEDLWID
                     ATAEYTRVGTLPSADTDRLALVIRAGEKALTRTPAMSSDKNRQLETREVFLAEYGPAR
                     VIETTETHGTIEGEFRSWYAGADNKARLDDLQSYVRSAYRAKSLEKYEHTASTDFSKP
                     YSMSLEMTDAPVGFTDLQTSAVGIKVSNITTRLPSYFTERLKEDSENANSDPRTTDVV
                     FEPFITEWRYRIRPPVGFQARKLPADGMQTLGPAKLSSEFKLAADGTVNATWRFDTVK
                     RRYTPAETNAFLAALRELKDAETQLISFDQVGVALRADGDFKGALRANDQLVSKYPRK
                     AVHRLRSATALLEAGLGIRAQREALAAVNFEPKSALAWKTLGWMLQHDAVGRRFGDGF
                     DRKGALAAYHRAQVLDPENADISADLAVLLEHDSHGVRYSPEADLEEAISNYQLRRAL
                     LSEEDAKDDDYGNNLLYALLYAHRYGELREALRKQPPGATQRALTLAAIGAESGSAKA
                     LEFARGMSDASDRRTALASAGNLLVRLREYSSAADLIEASTRGQTTTAASTQRIAMLR
                     KTQRNDGKAIKPADPRSVVLRWFMELLSAEKNEDGLRKLLAPGSEFLVDSPEFVAARR
                     GIVTGLRNQDLPYEVTTDILFSNVRVSVEGDDPGGYRVQLRMTGETQTFYVVKVGGDY
                     RILTAESLLGPVAVKAQERIDAGDFAGARRWLDWARVEQRPSNTDDPLAGPSFSRAWT
                     VGAETDLAAARAATAMLLADSGLGERAVPLLVAARGADPSAADKLSIDLALAHAYLDL
                     ERWTELREVASRLVAAVPNSAQSVRYQQWSMIQLGQWDAVEKVARERLARLPDDSLAR
                     EILVRRAEARGEFRTISGIMQPLIDSGRATASDYNQYAWTALLTQPVDERAVDAARMA
                     YDETQGKSYAIAHTLACVYAATGKPREARDLLLKGMEMVGIDRPDNSAWYGFGLVAEA
                     YGDTESARDYYAKVVKPKKGMIEPSSVYALSQARLTALK"
     gene            complement(10282..10950)
                     /locus_tag="VEW08_05075"
     CDS             complement(10282..10950)
                     /locus_tag="VEW08_05075"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HYJ40140.1"
                     /translation="MSRKSYLWVLAAIYAVWWVLMAIKPHDFSDWLLENALVVFAVAL
                     LAFTYKSFPLSRVSYTCIFVFMMLHALGAHYTYAKVPYDQWFPVFEGGRNHFDRLVHF
                     LYGLLLAYPIREMFLRIGNVRGFWGYFLPLDLTMSTSMLYELIEWGAAEAFGGELGAA
                     YLGTQGDIWDAHKDMALASLGALIAMVVTALINRHMQRDFAGEWAESIRVKRKKPLGE
                     ETLR"
     gene            complement(10940..12364)
                     /locus_tag="VEW08_05080"
     CDS             complement(10940..12364)
                     /locus_tag="VEW08_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HYJ40141.1"
                     /translation="MTVDERINAFLAPVADWLGKVVFFSVPVAGAQLPLIVAWLIIGG
                     IVCTLAFKFVNLRGFRHSARVIRGDYSSRTHPGEASPFQALSTAVSGTVGLGSIGGVA
                     VAVTLGGPGAALWMMLAGFLGMSLKFAEVTLSVKYRRVRADGTVTGGAMYYVPEALGR
                     VGMPRVGKFLAGFFCVAAIGGSVTIFQVSQAYSQVREVTGFDQAFLFGCMVAIWIGIV
                     LFGGVKRIVYWTDKLSPFMCLLYIVACLVVLSVNYTNILPALATIVREAFVPDAVAGG
                     IVGALIQGFRRAAFANEAGIGSAPMAHATVRTNEPMSQGFAALMEPFLDTIIICLLTA
                     LVIVVSGVNETSSNVGIALTSDAFATVVPWFPAVLAVVAVLFALSTVLAWGYYGEQAW
                     MWLFSEKKASRIAFRLFLCGMLSIAATFPLDQVVNIVDACSFCMALPNILAIYLLMPE
                     LRADLANYWQRVVLKGAVEKVNVA"
     gene            12579..13001
                     /locus_tag="VEW08_05085"
     CDS             12579..13001
                     /locus_tag="VEW08_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40142.1"
                     /translation="MIREGRVFGFALGLLATFLLATPLRATAGDKLRESASKTAAPLD
                     IPHTTDLELTARANRKRAPGNIEKPDFLEPTPGLQPLTFPKNSDIRARILTPELRRTP
                     LVGWIAANLYRGRDEKGWCLEADPGQGEYIVFYRLHWK"
     gene            complement(13049..14146)
                     /locus_tag="VEW08_05090"
     CDS             complement(13049..14146)
                     /locus_tag="VEW08_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYJ40143.1"
                     /translation="MSLHKLLPEQTDATAPMSVHVSLIQELYDVSDAIVRTRAIQAFD
                     HAAKAYPIHRIVRCAVRRKIGPVFDDLALQMECAAQRMGVGSLLLDGPGVFIGGEGYT
                     KGDYVSCTFRVWTDSLARLEEVRQRLFRVIGDKRLRDQMFVVDWNFCNSRGTLISTSF
                     DEIADDLLLEEAYPSLGKSVGEFVEGFLNARETVLILQGPPGTGKTRLVRAILAAMSR
                     RKGESAKVMYTADKRALENDEIFVDFITGSHDAFVIEDADHLLKARTSGNFELHRFLG
                     IADGVVRAQSRKVIFTTNLPNINDIDDALLRPGRCHAVKNLRSLDLGEALRLAEKICG
                     ADAEAAARASGKLSAAGSKYYSVAQVYRACT"
     gene            complement(14412..14885)
                     /locus_tag="VEW08_05095"
     CDS             complement(14412..14885)
                     /locus_tag="VEW08_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40144.1"
                     /translation="MRALLVSVLCLTSGLAAATNYRDVQRAVGDDAMLQTAGVSPADA
                     QLRQQCSAAILETDDPPDFFDCVYVQTDKDLNLFSLEDGYLMSELQLKLHNMDGVALQ
                     HMGRYSQVQIFSGDRVTALYIHGKSWIDPAATKGVYQWLTQHGVPAREPRKWIGP"
     gene            complement(15283..18363)
                     /locus_tag="VEW08_05100"
     CDS             complement(15283..18363)
                     /locus_tag="VEW08_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40145.1"
                     /translation="MREPSGYGIHGRSPGCPFSLARALARALAILALLAASAGVHAQA
                     ITRYVRDTGNINFVSTGGSLRNSNTNTCNLNATSTTALSGIPAGTTIRNAYLYWGGSG
                     GTADTTVTFNGNAITATRTFAATYTGVTPSLPYFGAFANVTSIVTTTRNGNYTFGGLT
                     VVTGSPHCDVSAVVSGWSLIVIYESATERLRAINLYDGLAPFRGSQVILTPDGFRVPA
                     TNIDGRIAVFTLEGDPANSDQMNGIDEALRYNGSLLDDGINVAGSVPTIQQFDGTINT
                     QGIATSYGIDVDQYNISALLAPGQTSGTTTYSAGADLVLLMAQIVSATSDPAVDLSVT
                     LSHTGTFVSGGTGTYTITVSNAAGTEREDNTVTVVDTLPSGLTYASSSGTGWSCSAAG
                     QVVTCTHAPTINPGASLPPLSLVVNIAEAAAASVTNSVTVDSPSYEVVTANNTATDVT
                     ATLDPNVSTSTKSVVDVNGGEASPGDTLRYTITLTETAGGLARNVSLTDDVPDDVTFA
                     GYVSLPAGAVGLFAAAPAGANGNGIVNVTGINVPASSSVTVVFNVTVVAGTLPGTNIN
                     NTATLNNPNGPENNPAAPAVVVNPSLVPSAGTKFVYLHRTVAGARSLSRIVPTAADTN
                     EAVANGTPDSWTITPALQTAFALSASPIPVRLWLTRTGSTFPNSRNVTVTLTSSSGYT
                     TTVSNSITPPNSTTTPTLFSFNLPNTLVRNFPAGTTFTLTVSNAGSNNITIWPNGNGA
                     AGNNSRIEFPATTVINVDSISMYNAAFNGGAVQSTFYPGTNVFVRAQISDPFGSFDIG
                     SARITITDPANVVQVNNQLMTAQGAPATCNSTSAASCIFQYQYIVPASPSLGAWTVRI
                     TANEGTEGTVTDIGISNFTVVIPQPSLTIIKTSTVLSDAVNNTTNPKRIPLAVVRYDV
                     TVTNSGPGTVDAGTLVITDPIPADASMYVSTASGDPVEFLNGAPASGLTYTYASHVSY
                     SSAGVSGPWTYAPAPDVNGFDPLVRAVRISPAGVMSAAGAGNPSFTIQFRVRIN"
     gene            complement(18486..19154)
                     /locus_tag="VEW08_05105"
     CDS             complement(18486..19154)
                     /locus_tag="VEW08_05105"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HYJ40146.1"
                     /translation="MKSANRELVDRLAAEYVLGTLRYRARRRFERWLLSPQVGAIVKA
                     WEDRLAGLEPQLTPVTPSAAVWRGIENKLELRKMQRRPAMRWLGVAASLIFFVAVGVF
                     ITRPPAQPPMTAQSSLQTDPQTIYWRVEVLGDNQELNLRVQTVHDLPAGKSHELWALP
                     EGGAPVSLGVLPHTGDHHRVLNAAQRAALANSKQIAVSLEVEGGSPTGAPTEVLFAAP
                     LSRA"
     gene            complement(19151..19717)
                     /locus_tag="VEW08_05110"
     CDS             complement(19151..19717)
                     /locus_tag="VEW08_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ40147.1"
                     /translation="MNRTDTLPPDIELARLLQQCAAADTVAFRTLYDKTSPILFARLL
                     RMLRRRSVAEEVLQDVFVRIWQRAAQFEAHRGRALAWMVTIARYCAIDLMRRERMTLV
                     SDDAIAEVADEASGEPGGLEKPNNFDHCIGKLNDNTRQCLTLAFVEGRSHDEIARLTT
                     NPLGSVKSWIRRGLLALKDCLSSEGQTT"
     gene            19808..21031
                     /locus_tag="VEW08_05115"
     CDS             19808..21031
                     /locus_tag="VEW08_05115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HYJ40148.1"
                     /translation="MTSSRAALTFVLLSVFIDSLGFGIIIPSLPGVIMKLTGEPVSTA
                     ADWSGYLMAVYALLQFFMAPVFGNLSDRYGRRPILLGSLFAFGVDFLLTGLATSMAWL
                     FIGRAFAGVFGASFATAGAYIGDISDDTNRARNFGFIGAAWGSGFTLGPVIGGFVAEH
                     FDVRAPFFVAAALALANVAFGLFALPESLPPERRRKFEWARANPFGAFKSLTHLPMVA
                     GLLFAVFLYQVAHDSLPAVWMFFTQHKFGWGPSETGWSLTFVGVMTVIVMGGLTGYVV
                     PRLGERRAIVVGFLLMTIGFLGYALVPQGWMIYPAIVFGSLGGIANPAMQSVMSKQAG
                     PSSQGELQGAVASLASIAAIISPIFMTQLFSHFSVPGAPVQLPGAPYLVAGALVFCCV
                     LIALRTVPGRETGKT"
     gene            complement(21084..21653)
                     /locus_tag="VEW08_05120"
     CDS             complement(21084..21653)
                     /locus_tag="VEW08_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40149.1"
                     /translation="MTELSALWLPVLLGAVVCFIASSIIHMTPLWHKNEYPQLPDEEK
                     ARAAIAALNVPPGDYMLPRCKSMKEMGSEDFKAKLRQGPKWIITVMPPGEGGMASSLV
                     FWFLFQLLVLVFAAYVSSHALAPGAHYHEVFRYIGATAFMGFALAQIPQSIWYRRAWS
                     TTIKLMFDGLIYACLAAGVFGWLWPKALE"
     gene            21771..22622
                     /locus_tag="VEW08_05125"
     CDS             21771..22622
                     /locus_tag="VEW08_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HYJ40150.1"
                     /translation="MRRPLALLSWIVIAASFSAHAEDTVDSVQAQRALVDAQVARLSS
                     EPDPHGRVYFLGFAGVGEERVFAEEIKLAAQRVGEKYGSAQRSVLLLNDRRDLATWPL
                     ASTSSLRYSLTEIARVMNRDEDVLFLALSSHGSRDTTIAVSNTGMTEQGLSAHTVAKL
                     LKESGIKWRVIVVSACFSGAFVKPLADKNTIVIAAAAKNRTSFGCSDQRDLTYFGEAF
                     YRDALPHSTNLRVAFEAARTEIRRREKDEEFTPSKPQGYFGPMMEEKLRAIEQARAPL
                     LRPGQGR"
     gene            22732..>22934
                     /locus_tag="VEW08_05130"
     CDS             22732..>22934
                     /locus_tag="VEW08_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40151.1"
                     /translation="MESFSVRTTLTQQDWHAYLAAANIRIANAKRADASLLMRVAPTA
                     MLFTVIAVVVFMLLFKPPLLKPEG"
BASE COUNT         4096 a         7227 c         7508 g         4103 t
ORIGIN      
        1 ctcccgcgga ccccatcgta gatccattcg ccgacgtcga ccgcgcggcg agcgcggcct
       61 ttaccgcgca gggcatatct ggaatgggcc tggctatcta cgacgcgaac ggcgtgaagc
      121 gcttcgaaaa gatgtacggg actttctcgg ccgaccagcg cgtggcgatc gcatcgtcat
      181 cgaagatggt ggcgggcctg gtgttgatgc ggctcatcga ccagggattc ctgacgctgg
      241 attcaaccac gggcacggtg ctgggctgga cggggccgca ggccgcgatc acgttgcgcc
      301 agttgctgtc gttcacgtcc gggctcgagc gcgaagcgcc gtgcactttg ttagcgggca
      361 tcacgctggc cgattgcgtg gcggcgattt cgcagcaggc gatggtggcg gcgcccggca
      421 cgcggttcga ctatggcagt acgcatctgc acgtggcggc gcgcatggcg gaggtggcca
      481 cgggcgccac gtgggcaaat ctctttgcca cgcaactgaa gacgccgttg gggctcactt
      541 cgccggacct cgcgtggtac accgcgccgc ggcaggcggt gggcatttcc aatccgctcg
      601 tcgcgggcgg cattcgcgcc acgatgaatg aatacgcgag gttcctggcg ctcgatttca
      661 atcgcggcgt gtaccagggc aaccggttga tcggtgatgc gctattcacg gcgcagtcga
      721 cggagcccta tccgaatgcc gtgatcggca actcaccgtt cgtgaacgcg ggcatcaact
      781 ttcactacgg cctcgcggcg tggctcgagt gtgtgccgcc ggcgaccaac tgtgcggtgc
      841 tttcgtcgcc gggcgcgttc ggcttcacgc cgtggttcga tcgcgatggc ggctactacg
      901 ccatcctcgg catggaagtg acggagtcgc agtcgggcgt cgtggcgttc gccgtgaatc
      961 ttgcgcaaga gctgaagccg ctgatccgct ctgcgctgtg aatgggggac aggcaccgca
     1021 gaggcgcctg accccattac gcggcgacct ttcggcaggt cacctcgccc tcgagcttca
     1081 cgccggtggc ggtctcgccg ctgaaactca gcagcacgtt gttgccctgg gtcatgagcg
     1141 tggtcctggc cttgccggta atgcctttgt tggcgcgctc ggccttctga aatggcttca
     1201 gcgccgagtc gggccgtgtg tcgatttcat acgtggtgct cgacatgccc ttcccgccag
     1261 ccatggcgcg tttgtccgcc accaccagca ctatctggat ttcatgcgcg tgtttctcat
     1321 cgatgtttgg catgtccagc gacaacgatg agttctcaga cgacaacacc accgagagcc
     1381 cgagaggctg gtcgctgaac gcggcgatgg cgcaggcctc gcgcggcgcg aacgcgtacc
     1441 tgcccgcgtg cgggccggtg gccacgctga tgctggcgct gctccccatg cccgcaggcg
     1501 cctgcggcat cgccggcgcg gcgatgagcg cgaacatcgc gcccgaaaac ccgactaaca
     1561 atgtgcgtgg cattttcact ggatgttgta gaccaggttg acggtgatca aggtatcggt
     1621 ggtctcggag agcggtggcg gatcggtgtt gtagcgaatc ccgtagccta ccgccaacgc
     1681 cagcgtcttc gtcatgctga cctgcaacgc caggtcattc tgcagcgcgg tattctcgga
     1741 tccggactcc gccaggaact tgttggtgat cttggtgctt tcggtgaagt tgtgttcata
     1801 ccccgaagcg aacgtgacga cgggatcgct ctcctcctcg ccttcgatgc gatcgatgac
     1861 ttcgccggca tcggtcctga tcaggatctc ttcctgcaat tgccggtaac cggcgccgag
     1921 cgagaacgac agcttggtaa ccggtgtgtc gatgaacttg taagccgcgc ccgtggacac
     1981 cgtagcctgg tagacgaagc cgctgaactt gtctttctca ccgcgcagcg cgccaaacca
     2041 cgacagccga tcgttgatct tgtagtcgag ctggtaactg gcttcgtagc gctcggcgtt
     2101 ggtgaattcg ccgttattgc cgtatagcgc gctcacgaag aatgcgttga tccatttttc
     2161 cgactcacgc ttgagatcga acttggtatt ggccgaggtg gactcggcgt taccgctcga
     2221 ctgcagatag ccgagctcgg ccttgccggt ccactgcgcc tgcgcggcgg gcgcgatcag
     2281 agccgcgcaa agaacgatcg aactgaattt gaaacccaca gaacttcctc gcggtcattg
     2341 ttagtgccgc gggagtctat cgggcgccac cggtcgctgc tcggcggcgc gtgattttgc
     2401 gggaaagtct tacagaatca ttctgcgttg ccgcattacc agcggtagct gacgcctagc
     2461 gaagtcacac tggtctcgcc gagattgtag acaccgaagt cgccttccag cgcggaatcc
     2521 aggaagtctt cctggttgta ctcgctgtag tcgagcacga ctccccaatg ttcgttgaag
     2581 cggtatccgc cgccgattcc gtagatgaat gccgtgttct cttcgtcggc ttcgaggcca
     2641 tccccgccga tctcatccaa ctcgaagtcg atcttgcccc agcccagccg tgcatcgagg
     2701 taccagtgtt cgccgatcgg cagaacaccc accacggaaa cgaggagccc gctgctggac
     2761 gtgcgcacat caagcggaca cgagccgggc gcaccgacgg ggcagtcatc cgggtcgaag
     2821 tgactttcga tttcacccag gtcgacgtag gcggcttcga tcgcgacgaa acggccgaac
     2881 tggtaaccgc cgcgcagcct aaagcccgtg ctgctgccgc tcgagcgggc ggtggtggcg
     2941 ctaaggacgt tttcgctggt tttcgcgtcg gaccgggctt cgcccacatc gagcccggcg
     3001 taaaacccct gcccgtgcgc catgaccggc agcatcaagg ccagcacggc cagtagtttg
     3061 gtcggtttca ttgcagcctc ccctcaaatg aactcaatca actcaagtga taactaacct
     3121 tggtgccatc ctggctcacg tacttgaggc ggatgagctc gtcgatgatc agtgcgacgg
     3181 ttttgtcggg cagcgtgcca ccatgttgtt tgaagtagct ggcgatggtc tgcgtcagct
     3241 tcttggaggt ggcgggcttg ccgctgagtg aatgcaggtg cttgatgatg ggcgcgagct
     3301 tttccgcgtg cgggcgccgc gggctgggca cgtgcggcgt gggcttggca accttggaca
     3361 cgggcgcggc ggggcgcggc gtcgcgtgtt tcggaacttc ggtaatggtc ttcacgcgtt
     3421 tgcagctgct gcccttggcg cgcaggtatt cgatgagcgg atcgaagtcg gtgtccttcg
     3481 agacgatgtg gatcattgcc cccggctcct tctcgagcag gcggccgacg tagtacgcga
     3541 tgtgcatgtc cacggcgttt tttccggtgc gcgcgatcct cacgtattcg gcgctggcgc
     3601 ccagcatctg catggagtgc gacagctcga aggaaatgcg tcccttcgcc tgggcggcgc
     3661 cgacgaaaac cttcacgcga aacgggccat tggcgagcgc cgcgaggctc tcaggttgca
     3721 cgttttcgaa gtcgaccagg acgtagttag ttgccatggg cgcgattgtg cacgtttgcc
     3781 ggcgcgaatg taggacgact ggtggaatgt cccggacgag ccaatgtccc cggattcgat
     3841 ggctgcaaat gacgcgcggg tgtctgccgg tgtcactaga ttggaactgg tcgaaatcga
     3901 aatggtgcca ggcaccgttt gctggaaatc ggtgcctggc accgtttagt taaagatgcg
     3961 gcgcgtcatc ggcgcgaatt ctgtgaccat tttcccaagc gcgcgatgaa tctgaacaaa
     4021 tccttgcgtc cactcgaaat cccttacagg atgtcaccgg tgtcattttt caggccggtc
     4081 tgcgggcctg tgggttttag gcggttttcg cgagggaaat cgatgttttt accctgcctg
     4141 gcgcgggcgc ttgcttattt gttgtccatc ggtttcctgc tgggaaccgc tagggcggcc
     4201 acccccgttg ccgatggcgg ccgcgagatt ctgggcatta acgacggctg gggggcggtt
     4261 cccacggcgt cgctgcccat ggggaccacg gggggctctg cggcggctcc cgcacgcacg
     4321 gtagcggtaa cgaaccgcaa ggaactggtg gcggctctcg cctggcccga cccctcgccc
     4381 aagctcatct atataaaggg tgtcatcgac gtgaacgtgg acgacgccgg caaggtgctc
     4441 acctgcaagg actatgcgcg gccggatccg gcgacgggcg aaatgttttc catctacgcg
     4501 ttcatggcca tgtacgaccc cgaaggcccg catcgcaaga acaatccctg gggtggacag
     4561 gaagaagcgc gcatggcctc ggccgccgcg caggaagcgc gcgtgcgcat ccgcgttcca
     4621 cccaacacca ctctttatgg cgtgggcggc gatgcgacgc tgatcggcgc gtggctcgat
     4681 atccgtggca acacgggcag ccagcccatg aacgtgatcg ttcgcaacat cagtttccag
     4741 gacacggccg attgctttcc cgagtggtcg cccaccgacg gcttcaccgg caactggaat
     4801 gccgcctacg actccatctc gatcgcccac gccacgcacg tgtggataga tcacaaccgt
     4861 ttcgccgatt tgaggacacg cgatgacaca cagccggtct atttcgggcg gcccctgcag
     4921 gtccacgacg gtcacctcga catcaccgac gaatcggact tcgtcacagt gtcgtggaac
     4981 caattcacgt cacacgacaa gaccatgctc atcggtaatt ccgatagcgc gacagaggac
     5041 cgggaacatc tgcgcgtgac gctgcatcac aacctgttcg atggcaccgg gcagcgcatg
     5101 ccgcgggttc gctttggcaa ggtgcacgtg tacaacaacg tctaccgcgc cgaccggaac
     5161 actaactacc gctcgagctg gggcgcgggc accgaatcgc agatctacgc cgagaacaac
     5221 tacttcgaga tgagcaagac cttcgggccg caggaagtca tcgacggcaa gaagggcacg
     5281 cgcatgacgg tcaccggcaa ttgctggacg gatgaggaat cctgcgttcc cacggatttc
     5341 ctggccttgc acaacgcaca gttcgaaccg gacattcagc cggacgcggg ctggacgccc
     5401 accctttatg gtccggccaa ggcggctgaa cctgcgaaat cggcacaaga acgggtgctg
     5461 agcgaaagtg gcccaggacg ggcctacaac aacaaaaact gacagacgtc agggagatgg
     5521 gcatggggcg aggcgccgcg actggttttg gcgcctcgct ccatcgctct gaatggtgcc
     5581 gggccgggaa accgggaaaa ctcggacatc aacaccttcg cggagttttc ccggtttccc
     5641 ggcccggcac cattcagagc tccgagtact tcttcgcgct gccacgcgcc ttgtccgccg
     5701 ggtatttctg cgcgttgagc ttcatctttt cgcgggcggc ctcgaggagg ttcacgtcca
     5761 gcttgtcggc caggcgcacc aggtagttga gtacgtcggc catttcctcg cgcaccttct
     5821 gcagttcggc tggcgggagc gcgcgtgact ggtcttcggt cagccactgg aagcgctcca
     5881 gcaactcggc ggcctcgacg gccagcgccg aggccaggtt cttggggctg tggtactggt
     5941 cccagtcgcg ttcggcggca aattcccgca aggcgtcgcg gagctgggtc aggccgtggg
     6001 gattggatgt ggtcatgcca cgcgagacta caacaagccg tgaacgaatc gcgctttgcg
     6061 ccgcctcgtg cggcgctatc cttttgccgg tatgcgcagc ccgctctggt tattaaccac
     6121 ggtcctcgcc gtgttctccc tgcccgccgg cgccgccgag gcctgggatg cacccgcgtt
     6181 ctcggtcccg gcacgggacc tgctgcaggc ggccacggcc gtcaaacgcg agcgcgccac
     6241 cgccgtggtg gtcctgctcg acgaacgcac ctacgtgctc gatgagcagc accggctcac
     6301 cagcatctcg cggctcatct atcgcgtcga ctcaccggat ggcgtggaac gctgggccgc
     6361 cagcaccgcg cactggcaac cctggcacca ggcgaaaccg accatccgcg cgcgcgtcat
     6421 cacgctcgat ggccaggaac accagataga ccagaagctg ctgaccgacg ctgccaaccg
     6481 cagcgaagac aaccaggtct acgacgactc gcacacgctg gaagggccgc tgccagcggt
     6541 cgcgattggc gcggtcatcg aagaagagat cacggtacgc gatgagaaac cgttcttttc
     6601 cgcgggctcg gtgtatcgcg aatacatcgg ccggccgatg ccggtgcttc gtacgcgcat
     6661 cgtcatcgac gcgccggagt cgctgccgct gaaacgccac atgcagttgc tgccgcgcgc
     6721 ggtggtgcag gaagtccgcg ccaacggccg cgtgacctgg acgctggaac acggcgtgct
     6781 cgatgaaatg ggcgagatgg actcgaacct gccgccggac gcgccgtcgt ggcccacggt
     6841 cgagttcacg tcggcgacgt cgtgggaagc cgtggccgcg gcataccgcg gcatgaccga
     6901 ggcgcgcatt cgcaacgacg acgccaaacc gctgatcgcg ggtctcaagg caactaagtc
     6961 accctctcgc gaatacgttg cgaaagtggt ggaacgcctg catcgcgagg tgcgctacac
     7021 cggcgtggaa ttcgccgatg cgcggctggt ccccgaatat cccgccgaga cgctgcgccg
     7081 ccggttcggc gactgcaagg acaagtccac gttgctggtc gcggcgctgc gtgcgtcggg
     7141 catcgatgcg tacctcgcac tgctctcctc gggcgacgac caggatgttt caccggactt
     7201 gcccgggctc gggatgttcg atcacgccat cgtctacgtg ccgggcgtgg aagacctgtg
     7261 gatagacgcc accgcggaat acacgcgcgt gggcacgctg ccatccgccg acaccgaccg
     7321 gctcgcgctg gtgatccgcg ccggtgaaaa ggcgctcacg cgcactccgg cgatgagctc
     7381 cgacaaaaac cgccagctgg aaacgcgcga ggtctttctc gccgaatacg ggccggcgcg
     7441 cgtgatcgaa accaccgaga cccacggcac catcgagggc gaattccgca gctggtacgc
     7501 gggcgccgac aacaaggcgc ggctcgacga cctgcagagc tacgtgcgca gcgcctaccg
     7561 tgcgaaatca ctggagaaat acgagcacac cgcgagcacg gatttttcga agccctattc
     7621 catgagcctc gagatgacgg acgcgccggt gggattcacc gatctgcaaa cttcggccgt
     7681 cggcatcaag gtgtccaaca tcaccacgag gctgccttcg tatttcacgg agcgcctgaa
     7741 ggaagactcc gaaaacgcga attccgaccc gcgcaccacg gacgtggtgt tcgagccttt
     7801 catcaccgag tggcgctacc gcatccgccc gcccgtgggc ttccaggcgc gcaagttgcc
     7861 ggcggacggt atgcagaccc tggggccggc aaagctcagt tccgaattca agctcgccgc
     7921 cgacggcacc gtaaacgcca cctggcgctt cgataccgtg aagcgccgct acacgcccgc
     7981 ggaaaccaac gcgtttctcg cggcgctgcg tgagctcaag gacgccgaga cacagctcat
     8041 ctcgttcgac caggtgggcg ttgcgcttcg cgccgacgga gacttcaagg gcgcgctgcg
     8101 cgccaacgac cagctggtgt cgaaatatcc gcgcaaggca gtgcaccggc ttcgctcggc
     8161 cacggcgctg ctcgaggcgg gactcggcat tcgcgcgcag cgtgaggcgc tggccgcggt
     8221 gaatttcgaa cccaaatccg cactcgcctg gaagacgctc ggctggatgc tgcagcacga
     8281 cgccgtgggg cgccgcttcg gcgatggctt cgatcgcaag ggcgcgctcg ccgcgtatca
     8341 ccgggcgcag gtgctcgatc ccgaaaacgc ggatatcagc gcggacctcg ccgtgttgct
     8401 cgagcacgat agtcatggcg tgcgttattc gcccgaggcg gacctggagg aggcgatcag
     8461 caactaccag ttgcgccgcg cactgctcag cgaagaggac gcgaaggatg acgactacgg
     8521 caacaacctg ctgtacgcat tgctgtacgc gcatcgttac ggcgagctgc gcgaggcgct
     8581 gcgcaagcag ccgccgggcg ccacgcaacg cgcgctgacg ctggccgcca tcggcgccga
     8641 aagcggcagc gccaaggcgc tcgagttcgc gcgcggaatg tccgatgcat cggaccggcg
     8701 cacggcgctt gcgagcgcgg gcaacctgct ggtgcggctg cgcgaatatt cgagcgcggc
     8761 ggatctcatc gaagccagca cgcgcggcca gaccaccacc gccgcgagca cgcaacgcat
     8821 cgcgatgctg cgcaagaccc agcgcaacga tggcaaggcg atcaagcccg ccgatccgcg
     8881 cagcgtggtg ttgcgctggt tcatggaact gctgtcggcc gagaagaatg aagacgggtt
     8941 gcgcaagctg ctggcgccgg gcagcgagtt cctggtcgac agccccgaat tcgtggcggc
     9001 ccggcgcggc atcgttacgg gcctgaggaa ccaggatctg ccctacgaag tcaccacgga
     9061 catcctgttc agcaatgtgc gggtgagcgt cgaaggtgat gacccgggcg ggtaccgcgt
     9121 gcagctgcgc atgacgggcg agacgcagac tttctacgtg gtgaaggtgg gcggtgacta
     9181 ccgcattctt acggcggagt cgttgttagg ccccgttgcc gtcaaggcgc aggagcgaat
     9241 cgacgcgggt gacttcgccg gtgcgcggcg ctggctcgac tgggcacgcg tggagcagcg
     9301 gccgtccaac accgatgacc cgctggccgg gcccagtttc tcgcgagcct ggaccgtggg
     9361 cgcggagacg gatctggccg cggcgcgcgc cgccacggcg atgttgttag ccgacagtgg
     9421 tctgggggaa cgcgctgtac cgttgctggt tgcggcccgg ggcgcggatc cgagcgctgc
     9481 cgacaagctc agcatcgatc tcgcgctggc gcacgcgtat ctcgatctcg agcgctggac
     9541 ggagctgcgt gaagtcgcct cgcggttggt ggccgcggtg ccgaactcgg cccagtcggt
     9601 tcgctaccag caatggtcga tgattcagct cgggcagtgg gacgcggtgg agaaggtcgc
     9661 gcgcgagcgg ctggcgcgcc ttcccgacga ttcactggcg cgcgaaatcc tggtgcgccg
     9721 tgccgaggcg cgcggcgagt tccgcaccat ctccggcatc atgcagccgc tgatcgacag
     9781 cgggcgcgcc acggcatccg actacaacca gtacgcgtgg accgcgctgc tcacgcagcc
     9841 agtcgatgag cgggccgtgg atgcggcgcg catggcgtac gacgagacgc agggcaagag
     9901 ttacgccatc gcgcacacgc tggcctgtgt gtacgccgcc accggcaagc cgcgcgaggc
     9961 gcgcgacctg ctgctgaagg gcatggagat ggtgggcatc gaccggcctg acaattccgc
    10021 gtggtatggc ttcggcctgg tggccgaggc gtatggcgat accgaatccg cgcgcgacta
    10081 ctacgcgaag gtggtcaagc cgaagaaggg gatgatcgag ccgagcagcg tgtatgcgct
    10141 gtcgcaggcg cggcttaccg cgctgaagta gcgagaggta ggtgccgggt ggggaaagtg
    10201 gggagtgcgc gctaagggtg tcgcatctgc cacccccaag tcgcactccc cactttcccc
    10261 acccggcacc tacttcggca cctacctgag cgtctcttcg cccagcggct tcttgcgctt
    10321 cacacggatg ctctcggccc actcgccggc gaagtcgcgc tgcatgtggc ggttgatcaa
    10381 cgcggtcacg accatggcga tcagcgcacc gagtgacgcc agtgccatgt ccttgtgcgc
    10441 atcccagata tcgccctgcg tgccgagata cgcggcgccg agctcgccgc cgaatgcttc
    10501 cgcggcgccc cactcgatga gctcgtagag catcgaggtg gacatggtga gatcgagcgg
    10561 cagaaagtag ccccagaagc cacgcacgtt gccgatgcgc aggaacattt cgcggatcgg
    10621 gtacgcgagc agcagtccgt acaagaagtg cacgagccgg tcgaaatggt tgcggccgcc
    10681 ttcgaacacc gggaaccact ggtcgtacgg caccttcgcg taggtgtaat gcgcccccag
    10741 cgcatgcagc atcatgaaca cgaagatgca ggtgtacgac acgcgcgaca gcggaaacga
    10801 cttgtacgtg aacgccagca gcgcaacggc aaacactacc agcgcgttct ccagcagcca
    10861 gtcggagaaa tcgtgcggct tgatggccat gagcacccac cagaccgcgt atatcgcggc
    10921 cagcacccac aggtatgact tacgcgacat tcactttctc cacggcgcct ttcagcacca
    10981 cccgctgcca gtagttggcg agatccgcgc gcagctccgg catgagtaga tagatggcga
    11041 ggatgttggg aagggccatg cagaagctgc acgcatcgac gatgttcacg acctggtcga
    11101 gcggaaacgt ggcggcgatg gacaacatgc cgcacaggaa cagccggaac gcgatccggc
    11161 tcgccttctt ctccgagaag agccacatcc acgcctgttc gccgtagtaa ccccaggcga
    11221 gcacggtaga cagcgcgaac agcacggcca cgacggcaag cacggccggg aaccacggca
    11281 ccacggtcgc gaacgcatcg gaagtcaatg cgatgccgac gttgctgctc gtctcgttga
    11341 ccccgctgac cactattacc agcgcggtca gcaggcagat gatgatggtg tcgaggaacg
    11401 gctccatgag cgccgcgaaa ccctgcgaca tcggctcatt cgtgcgcacc gtggcatgcg
    11461 ccatgggcgc ggacccgatg ccggcttcgt tcgcgaacgc ggcgcgccgg aatccctgga
    11521 tcagcgcacc gacgatgccg cccgccaccg cgtcgggcac aaaggcttcg cgcacgatgg
    11581 tcgcgagcgc cggcaggatg ttggtgtagt tgacggacag cacgacaagg cacgccacga
    11641 tgtacagcag gcacatgaat ggcgagagct tgtccgtcca gtacacgatg cgtttcacgc
    11701 cgccgaacag cacgatgccg atccagatgg cgaccatgca gccaaacagg aacgcctggt
    11761 cgaagccggt gacttcgcgg acctgcgaat acgcctggct gacctggaaa atggtgaccg
    11821 agccaccgat ggcggctacg cagaaaaatc cggcgagaaa ctttccgacg cgcggcatgc
    11881 cgacgcggcc cagcgcctcg ggcacgtagt acatggcgcc accggtcacc gtgccgtcag
    11941 cgcgcacgcg acggtatttc accgacagcg tcacctcggc gaacttgagg ctcatgccga
    12001 ggaacccggc gagcatcatc cacaacgcgg cgccgggccc gccgagagtg actgccaccg
    12061 cgacgccgcc gatgctgccg aggcccacgg tgccggacac cgcggtgctc aacgcctgga
    12121 atggcgaggc ttcaccggga tgcgtgcggc tggaatagtc gccgcgaatg acgcgcgccg
    12181 agtgtcggaa gccgcgcagg ttcacgaact tgaacgcgag cgtgcagacg atgccgccga
    12241 tgatcagcca cgcgacgatg aggggtagct gcgcgccggc tacgggcacc gagaagaaga
    12301 ccacttttcc cagccagtcg gcgacgggtg ccagaaacgc gttgattctt tcgtcgacgg
    12361 tcattgcgct gcagtgtaac gaaaggcgta ccaccggctc acattttgct gtcaaggcag
    12421 tgctgcctta cgggcggaaa atgacgccgc ggggggcgcg aaccagacaa tagtcctgac
    12481 ttttttcgcg aaaatcgctc ccgggcggat tcacctattg cgagccacgc gcgccgcctc
    12541 ctaatctgat cgcaacgaaa aaccggcgag gtcgttcgat gatccgtgag ggtcgcgtgt
    12601 tcgggttcgc gcttggcttg ctagccacct tcctgctggc cacgcctttg cgggcaaccg
    12661 ccggtgacaa actgcgcgag tccgcgagca agaccgcggc gccgctcgat atcccgcaca
    12721 ccaccgacct cgaactcacc gcgcgcgcca acaggaagcg cgcgccaggc aacatcgaaa
    12781 aacccgattt cctcgagccg actccgggcc tgcaacccct gaccttcccg aagaatagcg
    12841 acatacgcgc gcgaatcctg acaccggaac tgcgccgcac gccgctggtg ggctggatcg
    12901 ccgccaacct gtatcgcggc agggacgaaa agggctggtg tctcgaagcg gatcccggcc
    12961 agggcgagta catcgtcttc taccgcctgc attggaaata aaaaatgggg tcaggcaccg
    13021 ttttctagga aacggtgcct gaccccattt acgtgcaggc gcgatacacc tgagccaccg
    13081 agtaatactt cgagcccgca gcgctgagct tgccgctcgc acgcgcggcg gcctctgcat
    13141 ccgcaccaca gatcttctcc gccagtcgca atgcctcgcc caggtcgagg ctgcgcaggt
    13201 ttttcaccgc atggcatcgc cccggccgca gcaacgcatc atcgatgtcg ttgatgttgg
    13261 gcaggttggt cgtgaagatc accttgcggc tctgcgcgcg caccacgcca tcggcgatgc
    13321 cgagaaagcg atgcagctcg aaattgccgc tggtgcgcgc cttgagcaga tggtctgcgt
    13381 cttcgatcac gaacgcgtcg tgcgagccgg tgatgaagtc cacgaagatc tcatcgttct
    13441 cgagcgcgcg cttgtcggcg gtatacatga ccttggcgct ctcgcccttg cgcctcgaca
    13501 tcgcggccag gatcgcacgc accaggcgcg tcttgccggt gccaggcggg ccctggagta
    13561 tcagcaccgt ctcgcgcgcg ttcaggaagc cctcgacgaa ttccccgacc gatttgccca
    13621 gcgacggata ggcctcttcc agcagcaggt cgtcggcgat ttcgtcgaat gacgtgctga
    13681 tgagcgtgcc gcgcgagttg cagaagttcc agtcgaccac gaacatctgg tcgcgcagcc
    13741 gtttatcgcc gatcacgcgg aacaggcgtt gccgcacttc ttccaggcgc gcgagcgagt
    13801 ccgtccacac gcggaacgtg cacgagacgt agtcgccctt cgtgtatccc tcgccaccga
    13861 tgaatactcc gggcccatcg agcagcaggc tgcctacgcc catgcgttgc gcggcgcatt
    13921 ccatctgcag cgcgaggtcg tcgaacacgg gcccgatctt gcgtcgcacc gcgcagcgca
    13981 cgatgcggtg gatgggatac gccttcgcgg cgtggtcgaa cgcctggatc gcgcgcgtgc
    14041 gcacgatggc gtcgctgacg tcgtagagtt cctggatcaa cgacacgtgc acgctcatcg
    14101 gcgcggtggc atcggtttgt tccggcaata acttgtgcaa tgacattggc aagtccctgg
    14161 cgctggggcc tcgccccaaa tgacgaggcc ccggtggcgg cagccgaccg gggcctcgtc
    14221 agagacttgc cggagagcag ccgaatgccg ccctccgcaa atgaagggct agcaaatcat
    14281 gtggtcacga tcgcgttcac gcgggggtgc cgcaaacgcg tgcgtaaacc ggaattggag
    14341 gtagttagag gtcatggggc gcgcattcta ggcgcgattg cccggctgtc aagcggggcg
    14401 atgaaaaaag atcagggacc gatccacttg cgcggctcgc gcgccggcac accgtgctgg
    14461 gtgagccact ggtacacgcc tttggtcgcc gccgggtcga tccacgactt gccatggatg
    14521 tagagcgccg tcacgcgatc gccactgaag atctgcacct gcgaataccg gcccatgtgc
    14581 tgcagggcca cgccatccat gttgtgcagc ttgagctgca gctcggacat caggtacccg
    14641 tcttcgaggc tgaagaggtt caggtccttg tcggtctgca cgtagacgca atcgaagaag
    14701 tcgggcggat cgtcggtctc gaggatcgcg gcggaacact gttgtcgaag ctgcgcgtcg
    14761 gcggggctga cccccgccgt ctgaagcatg gcgtcatcgc ccacggcacg ttgcacgtcg
    14821 cgatagttgg tggcggcggc aaggccggag gtaagacaga ggacggagac cagaagggca
    14881 cgcatgggca cagaacctat gcgcatccgc ggcagtgcgg tgtcagacgg ctttacctca
    14941 ccttatgagg cgcgtcccct tttgtgcaaa tcaggaacgg ccccgaagcg gggcctgacc
    15001 ccatttcccg cgggcaaaaa aaaacgcgta caggcccttc cgttcctgta cgcgttctgt
    15061 ccagcaaaaa gtctcacgag gggggaggag aacattccgc agacgatcta gataaagcaa
    15121 tctgcgtgcc aactcgcccg gggcgaaaaa tagttttcag ggccgtaaat ccggcatctg
    15181 acggcccgtc tgaaaactga catcccagga tgggtgcaca tggcccgaaa tcgcttcgat
    15241 ccggtgcaat ggacaggccc gaccccaggg gccggggagc tatcagttga tccgcacccg
    15301 gaactggatg gtgaacgacg gattgccggc tcccgccgca ctcatgaccc ctgcgggcga
    15361 gatcctcacg gcccggacca gcgggtcgaa gccattcaca tcgggtgcgg gcgcataagt
    15421 ccacgggccg ctcacgcccg ccgacgagta cgacacgtga gatgcatacg tgtacgtaag
    15481 cccactcgcc ggtgcgccgt tgaggaactc gacggggtcg cccgacgcgg tcgaaacata
    15541 catcgaggca tcggccggaa tcgggtcggt gatgaccagt gtgccggcgt ccacggtgcc
    15601 gggtccgctg ttggtcacgg tgacgtcgta gcgcaccacg gcgagcggaa tgcgtttcgg
    15661 attcgtggtg ttgttcaccg cgtcggagag caccgtcgat gtcttgatga tcgtgagcga
    15721 aggctgcggg atgacgacag tgaaattgct gatgccgatg tcggtgaccg tgccctcggt
    15781 gccttcgttg gcggtgatgc gcacggtcca cgcgccgagc gaaggcgacg ccggcacgat
    15841 gtactggtac tggaagatac agctcgcggc gctcgtggaa ttgcaggtgg ccggcgcgcc
    15901 ctgcgccgtc atgagctggt tgttcacctg cacgacgttg gccggatcgg tgatggtgat
    15961 gcgcgcgctg ccgatgtcga agctgccgaa cgggtcgctg atctgcgcgc gcacgaacac
    16021 gttcgtgccc gggtagaaag ttgactgcac cgcgccgccg ttgaacgcgg cgttatacat
    16081 ggaaatgctg tcgacgttga tgaccgtggt ggccggaaac tcgatgcgcg aattgttgcc
    16141 cgctgcgccg ttgccgttcg gccagatcgt gatgttgttg ctgcccgcgt tactcaccgt
    16201 gagcgtgaac gtggtgccgg cggggaagtt gcgcaccagc gtgttgggca ggttgaagct
    16261 gaacagcgtc ggcgtggtgg tggagttcgg cggcgtgatg ctgttggata ccgtggtggt
    16321 gtagccggag ctggatgtca gcgtcaccgt cacgttgcgg ctgttgggaa aagtgctgcc
    16381 ggttcgcgtg agccacaggc gcacggggat cgggctggcg gagagggcga acgccgtctg
    16441 cagcgccggc gtgatggtcc aggaatccgg cgtgccgttg gccaccgcct cattggtgtc
    16501 cgccgcggtg ggtacgatgc gcgacagcga gcgtgcaccg gccaccgtgc ggtgcaggta
    16561 gacgaacttc gtcccggcgc tcgggaccag cgacgggttc accaccaccg cgggcgcggc
    16621 cggattgttc tcgggaccgt tggggttgtt gagggtggcc gtgttgttga tgttggtgcc
    16681 gggcagcgtg ccggccacca cggtcacatt gaacaccacg gtgaccgaac tgctcgccgg
    16741 cacgttgatg ccagtcacgt tgacgatgcc attgccattc gcgcccgcgg gcgcggcggc
    16801 aaacaagccg actgcacccg ccggcaagga cacataaccg gcgaacgtca catcgtcggg
    16861 cacgtcatcg gtcagcgaaa cgttgcgggc cagcccgccg gcggtctcgg tcaacgtgat
    16921 ggtgtagcgc agcgtgtcgc ctggtgaagc ttcgccgccg ttcacgtcga cgacggactt
    16981 ggtggacgtc gacacgttcg gatcgagcgt cgcggtgacg tcggtcgccg tgttgttggc
    17041 cgtgaccacc tcgtagctgg gcgagtccac cgtgacgctg ttggtcacgg aggcggcggc
    17101 ggcttcggcg atgttcacca ccagcgacag cggcggcagc gaagcaccgg gattgatcgt
    17161 gggggcgtgt gtgcaggtca ccacctgccc cgccgcactg cagctccagc ccgtaccgct
    17221 cgatgaggca taggtcaggc ccgagggcag cgtatcgacc acggtgaccg tgttgtcttc
    17281 gcgctccgtg ccggccgcgt tcgacacggt gatggtgtag gtgccggtac cgccggacac
    17341 gaacgtgccc gtgtgcgaca gggtcaccga caggtccacg gccggatcgg acgtggcgct
    17401 gacgatctgc gccatgagca gcaccaggtc cgcgccagcc gaataggtag tcgtgccgct
    17461 ggtctgcccg ggcgccaaca gcgcgctgat gttgtactgg tcgacgtcga tgccgtagct
    17521 ggtcgcgatg ccctgcgtat tgatggtgcc gtcgaactgc tggatcgtcg gcacgctgcc
    17581 ggccacgttg atgccgtcgt cgagcaggct gccgttgtaa cgcagcgcct cgtcgatgcc
    17641 gttcatctgg tcggagttgg ccgggtcgcc ctcgagcgtg aacaccgcga tgcggccgtc
    17701 gatgttggtc gccggcacgc ggaagccgtc gggggtcagg atcacctggc tgccgcggaa
    17761 cggcgccagg ccgtcgtaca ggttgatggc gcgcagccgt tcggtggccg actcgtagat
    17821 gacgatgagc gaccaaccgc taactaccgc gctgacgtcg caatgcggcg agccggtgac
    17881 cacggtcagg ccgccaaacg tgtagttgcc gttgcgcgtg gtggtgacga tcgaggtgac
    17941 gttggcgaag gcgccgaagt acggaaggct gggagtgacc ccggtatagg tcgccgcaaa
    18001 cgtacgcgtg gcggtgatgg cgttgccatt gaacgtgacc gtcgtgtcgg ccgtgccacc
    18061 ggatccgccc caatacaggt aggcattgcg gatcgtcgtg ccagccggga tgcccgacag
    18121 cgccgtggtg ctggtggcgt tgagattgca ggtgttggtg ttgctgttgc gcagcgagcc
    18181 gccggtgctg acgaagttga tgttgccggt gtcgcgcacg tagcgcgtga tggcctgcgc
    18241 gtggacgccc gcgctcgcgg ccagcagcgc gagaattgcc aatgcccggg ccaatgccct
    18301 tgccaatgaa aagggacacc cggggctgcg gccgtggatg ccgtaaccgg acggctctct
    18361 catggccacg attctggcag tcacgctgca gtgcaggcgg gcgcagctct cataatccgg
    18421 gagcccgtaa gtcccggatt gagaacgact tcacacccgc ctgtcggtcg aaacacccgc
    18481 gcggatcagg ccctcgagag tggcgccgcg aacagcactt cggtcggcgc gccggtcggt
    18541 gagccgccct cgacctcgag actgaccgcg atctgcttcg aattcgccag cgccgcgcgc
    18601 tgcgcggcgt tgagcacgcg atggtggtcg ccggtgtgcg gcagcacgcc aagcgacacc
    18661 ggtgcgccac cctccggcaa tgcccacagc tcgtgcgact tgccggccgg gaggtcgtgc
    18721 accgtctgca cgcgcaggtt gagctcctgg ttgtcgccga ggacctcgac gcgccagtag
    18781 atagtctgcg ggtcggtctg cagggacgac tgggcagtca taggcggctg cgccggcggc
    18841 cgcgtgatga acacgcccac cgccacgaag aagatcagcg aggcggcgac gcccagccag
    18901 cgcatggcag gccggcgctg catcttgcgc agctcgagct tgttttcgat gccgcgccac
    18961 accgctgcgg atggcgtaac cggtgtgagc tgcggttcaa ggcccgcgag cctgtcttcc
    19021 cacgctttca cgattgcccc cacctgcggc gaaagcagcc agcgctcgaa gcgccggcgg
    19081 gcgcgatacc gcagtgtgcc gagtacgtat tccgccgcga ggcgatccac cagttcgcgg
    19141 ttggcgctct tcatgtcgtc tgcccctcgg aagagaggca gtccttgagg gcgagcaggc
    19201 cgcggcggat ccagctcttc acgcttccca gggggttggt ggtgaggcgt gcaatttcat
    19261 cgtgcgagcg cccttcgacg aaggccagcg tgaggcactg gcgcgtattg tcgttgagct
    19321 tcccgatgca gtggtcgaag ttgttaggct tttcgaggcc gccgggttct cccgaggcct
    19381 catcggcaac ctcggcgatg gcgtcgtccg agaccagcgt catgcgctcg cggcgcatga
    19441 gatcgatggc gcagtagcgc gcgatggtca ccatccaggc gagcgcacga ccgcgatgcg
    19501 cttcgaattg cgcggcgcgc tgccagatgc gaacgaagac gtcctgcaag acttcttcgg
    19561 ccaccgagcg ccgccgcagc atgcgcaaca ggcgtgcgaa gaggatgggt gaggtcttgt
    19621 cgtacagcgt gcggaaggcc accgtgtccg cggcggcgca ctgttgcagc agccgggcca
    19681 gctcgatatc cggcggcagg gtgtcggtac gattcatgct gagttctacg tggctgattt
    19741 gacggtggat gcaacgccat caaaatctag cacgcaatag gacatatacc cgacaatgcc
    19801 ggcccccatg acttcatccc gcgctgcgct gacgttcgtc ctgctgtctg tattcatcga
    19861 cagtctgggc ttcggaatca tcattccctc gctgccgggc gtgatcatga agctcaccgg
    19921 cgagccggtg agcaccgccg cggactggag cggctacctg atggcggtgt acgcactgct
    19981 gcagtttttc atggcgccgg tcttcggaaa cctgagtgac cggtacggca gaaggcccat
    20041 cctgctcggc tcgctgttcg cgttcggcgt ggactttctg ttgacggggc tagccacctc
    20101 gatggcgtgg ctattcatcg gccgcgcgtt cgccggcgta ttcggcgcct cgttcgccac
    20161 cgccggcgcg tacatcggcg acatcagcga cgacaccaac cgcgccagga acttcgggtt
    20221 catcggtgcc gcgtggggca gcggcttcac gctggggccg gtcatcggcg gcttcgtggc
    20281 cgaacacttc gacgtgcgtg cgccgttctt cgttgcggcg gcgctggcct tggcgaacgt
    20341 cgcgttcgga ctgttcgcgt tgccggagtc gctgccgccc gagcgccggc gcaagttcga
    20401 gtgggcgcgc gccaatccgt tcggcgcgtt caagagcctg acccatctac ccatggtcgc
    20461 gggcctgctg tttgccgtgt tcctctacca ggtggcgcac gattcgttgc ccgcggtgtg
    20521 gatgttcttc acgcagcaca agttcggctg gggcccttcg gagaccggct ggtcgctcac
    20581 gttcgtcggc gtcatgacgg tcatcgtcat ggggggtttg acggggtacg tcgtgcccag
    20641 gctcggcgag cggcgcgcca tcgtcgtcgg gttcctgctg atgacgattg gcttcctggg
    20701 ttacgcgctc gtgccgcagg gatggatgat ctacccggcc atcgtgttcg gctcactggg
    20761 cggcatcgcc aatcccgcga tgcaaagcgt gatgtcgaag caggcggggc cttcgtcgca
    20821 aggggaattg cagggcgccg tcgccagtct cgcgagcatt gccgccatca tctcgccgat
    20881 cttcatgacg cagctgttca gccacttctc ggtgcctggt gcgcccgtgc aattgcccgg
    20941 cgccccctat ctagttgcgg gagcgctggt gttctgttgc gtgctgatcg cgctcaggac
    21001 ggtgccgggc cgggaaaccg ggaaaactta gccaccgaac ccactgcgtc gcgttgccgg
    21061 acttcaatcc ggcccggcac cgcctactcg agggccttcg gccacagcca gccgaagacg
    21121 ccggcggcga ggcacgcgta gatgagcccg tcgaacatca gcttgatcgt ggtgctccac
    21181 gcgcgccgat accagatgga ctgcggtatc tgcgccagcg caaagcccat gaacgccgtc
    21241 gcgccgatgt atcgaaacac ctcgtgatag tgcgcgccgg gtgcgagcgc atggctcgac
    21301 acatacgcgg caaacaccag cacgagcagc tggaacagga accagaaaac caggctcgac
    21361 gccatgccgc cctcacccgg tggcatcacg gtgatgatcc acttgggccc ctgtcgcagc
    21421 ttcgccttga agtcctccga acccatctcc ttcatggact tgcagcgcgg cagcatgtaa
    21481 tcgccgggcg gcacgttgag cgccgcgatc gcagcgcgcg ccttctcttc atcgggcagt
    21541 tgcgggtatt cgttcttgtg ccagagcggc gtcatgtgaa tgatggaact ggcgatgaag
    21601 cagacgacgg cgcctaacag gacgggcagc cacagcgcgc tgagctcggt catgacgatc
    21661 tcccttgtta tccgacgccg cgcattctgc aaccgtttgc ccctctcaac aaccagcgag
    21721 cctcaacaac cgcttgccgc aaccgttcat acaccgccac actgcccgcc atgcgcagac
    21781 cccttgccct gctgtcgtgg atagtgatcg ccgcctcctt cagcgcgcac gcggaggaca
    21841 cggtggactc tgtccaggcg cagcgcgcac tggtcgatgc gcaggtcgcc cggttgtcga
    21901 gcgaacctga tccgcacggc cgtgtgtatt tcctggggtt cgcaggcgtt ggcgaagagc
    21961 gagtgttcgc cgaggaaatc aaacttgcgg cgcagcgcgt gggtgagaag tacggatcgg
    22021 cgcagcgctc ggtgctgctg ctcaacgacc ggcgcgacct cgccacgtgg ccgctcgcct
    22081 cgacgtcatc attgcgttac tcgctcacgg aaatcgcgcg cgtcatgaac cgcgacgagg
    22141 acgtgctgtt tctcgcgctg tcatcgcatg gctcgcgaga cacgaccatc gccgtttcga
    22201 acaccggcat gacggagcaa ggtctcagcg cccacacggt cgccaaactg ttgaaagagt
    22261 ccggcatcaa gtggcgggtg atcgtcgtct ccgcctgctt ctcgggtgct ttcgtcaagc
    22321 cgctggcgga caagaacacc atcgtgatcg cggctgccgc gaagaaccgc acttcgttcg
    22381 gctgttcgga ccagcgcgac ctcacctatt tcggcgaagc cttctatcgc gatgcgctgc
    22441 cccactccac caacctgcgc gtggcgttcg aagcggcacg cacggaaatt cgccggcgcg
    22501 agaaggacga ggaattcaca ccctcaaagc cgcagggata tttcgggccg atgatggaag
    22561 aaaagcttcg cgccatcgag caggctcggg caccgctcct gcgcccgggc cagggccggt
    22621 agtagcggaa gtcgtggccg cagccgtgcg gtcggtcact gttcgggcag cgcgaaagag
    22681 gcgcgggccg tcgatctgcc agacttccgg aaaccccggg atacacgttg catggaaagt
    22741 ttcagcgttc gaactacttt gacccagcag gactggcacg catacctggc ggccgccaac
    22801 atccgcatcg caaatgcgaa gcgggcggac gccagcctgc tcatgcgcgt tgctccgacg
    22861 gcaatgctgt tcacagtgat tgcggtcgtc gtgttcatgt tgttgttcaa gcctccgctg
    22921 ttgaagccag aagg
//
LOCUS       DASQJS010000124         6389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4146615, whole genome shotgun sequence.
ACCESSION   DASQJS010000124 DASQJS010000000
VERSION     DASQJS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4146615"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1186)
                     /locus_tag="VEW08_05135"
     CDS             complement(59..1186)
                     /locus_tag="VEW08_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HYJ40152.1"
                     /translation="MPITIGALRESAPGETRVSLVPEVVDKFLAAGARVVLEPGAGIR
                     AQFPDALYKKGEWLASSADVLSQSDVLLTVAPLSLEQIAQLKPGSVVVGFLQAHTKHA
                     EVKALRDRKITSFAVELIPRISRAQSMDGLSSQASLAGYKAVLIAANNLEKFMPMLTT
                     AAGTIRPSQALIIGAGVAGLQAIATARRLGAVVEAYDVRSATREQVKSLGAKFVETGV
                     SADGVGGYARELTAEEKAKQQEALDARIAASDAVISTASVPGRKAPVIISKAAVERMR
                     AGSVIVDIAAEQGGNCELTKAGETVIHQGVKIIGPVNVAGTLAYNASEMYARNLLNFL
                     KPAIDKAGDLKIDWTDEVFAGAVLTHDGAIKHEATRKAVEG"
     gene            1306..1980
                     /locus_tag="VEW08_05140"
     CDS             1306..1980
                     /locus_tag="VEW08_05140"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HYJ40153.1"
                     /translation="MLGIDLEKADRGDGSDLRLLASDLGRALSQLVLRTDVSGMPLEW
                     IDYKEAARLYHQEQVAYTCGSPLFKLYGGVSARTGFRTVLEVNSIVATVGHTGNPGNT
                     RHDYVPPLNNQTLFRRDAHFCLYCAQRFPVRELSRDHIRPFSQGGRDVWTNVVAACRR
                     CNNLKASRTPEQAKMQLIAVPFTPTYAEYIFLKGRRVLADQMEYLRAHFPRHSPLHAR
                     IKRYLS"
     gene            complement(2036..2788)
                     /locus_tag="VEW08_05145"
     CDS             complement(2036..2788)
                     /locus_tag="VEW08_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40154.1"
                     /translation="MKSLDRNSLEEQTRLVSRYVAGDLSRSERAEFEAWLVASPELAA
                     EVEMERRLRRGIASAARRGWLNRSAPEPRRERQWQFALAASVLVALGLGLGVFLPRDE
                     SQAPGTNRVASSVQRVAASSTFRLSNVRGADSIPDINVSLTDAPGELIIKPDVVVLTC
                     EDGAIELECVGGHSPQQPQYAEYEMDLVNRRGATLAWRSSRQAPVGRTQLAFTLRDPG
                     SLDAGDYDLIVRGHSPEHEEVVARFWLRVAAQ"
     gene            complement(2801..3469)
                     /locus_tag="VEW08_05150"
     CDS             complement(2801..3469)
                     /locus_tag="VEW08_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ40155.1"
                     /translation="MSTIPVEEIERIVAAALTGDRLAESRMLVALRPGVLAVLRFGAF
                     HRWIDAEDLTQETLHIVVERVRARTVDDPRKVFAFAAATARNLALNAARKALRQQTLV
                     DSELVDELAQNLEMEQSDLSESDDRHLAQAVAELLEELPTERDRQLLTRFYLDGTDKQ
                     QLCHELGLSPKHFDRVLMRARSRLRTIIERRAPHLALFPRISSILLPLMMSFTVWVRH
                     FGSW"
     gene            complement(3494..5014)
                     /locus_tag="VEW08_05155"
     CDS             complement(3494..5014)
                     /locus_tag="VEW08_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYJ40156.1"
                     /translation="MLLPLAAATTFGASCALAAAPNLCAHGGQWAPTAARAPVAVIEY
                     LGALGDARFDLRAACAHWAGMFSQRHRSAANAAQRNRQVPARLIVFPAEGTSQRQLVD
                     WLEHTGYVRDVELARNFVVVVFSRYTPTERLIAIINSPLVGYSEPDCDGPDYLTTSME
                     LPPAAVSASRCWLRAARSTFPNDPCLDELWGHRKIGWDSGIAARSLPRIVAVLDSGID
                     AAHEDLARNVVLHLHAHETPGSQGGRLNARCATSGRCYAHGTEMAGTIGGRMDNGIGV
                     AGVAPNSRLLPIVISRVDRGLLARISTIAAGIDAAVDDGAEIINISAKWPVDSRAISE
                     SIIAAVGGSSSARRLMVTGYATSLDRDDTVREYFPSQYRCLPGVIAAVPTDMRGDELF
                     NPRGHPDANDGRIQAPGVDIVVTTTENSERGYALSESAGASSAAAYVSGAVALVWGSP
                     PLNKCDAQQIKQLLFCRSKDSNSTRYPWVNVEFLRELANLDPGADCRSAMAALECN"
     gene            complement(5059..5607)
                     /locus_tag="VEW08_05160"
     CDS             complement(5059..5607)
                     /locus_tag="VEW08_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40157.1"
                     /translation="MYGKLKIDGRKLGIWTAAAFLGFGATLATAQENAPDSVLAGWLG
                     KEYTVASSTLNDHMPAGGKLTFVFDAEENVVRICTRNSSKQRSPWNMDFATPCGVTML
                     FTRGTRFCTADDVKAGNAEVLSSCHRLRSRDIAMRPSKVKGTVELNDMIAFLVQENGK
                     PVMSILVDSPARVTDGGIIQIK"
     gene            complement(5660..>6389)
                     /locus_tag="VEW08_05165"
     CDS             complement(5660..>6389)
                     /locus_tag="VEW08_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYJ40158.1"
                     /translation="LAMEKARPAKSHNTRVAVISDPVYAADDRRLRIAQGGGAGTLRG
                     ARRASPNNLTRLPYSALEASAVTKAFGSADTIQLSGFDATAERVLKLPSSELAVLHFA
                     THAVARQDSPEQSALFLSEYTPEGAQLPDSRLTANEISRSGLRADVVVLSGCATGDGS
                     ALRGEGVLGLTYGFLANGSHSVVATLWPIEDASTARFMNEFYQAYRGSGRAADALRTA
                     QLRTRSSAAPAVWSSFVVRANEFP"
BASE COUNT         1155 a         2018 c         2037 g         1179 t
ORIGIN      
        1 tagccggtga aagccgccag catgaagatg tagagagcaa cgattccagt cattgtgatt
       61 agccttccac tgccttgcgc gtggcttcgt gtttgatggc gccgtcgtgc gtgagcacgg
      121 cgccggcgaa tacttcgtcc gtccagtcga ttttcagatc gccggccttg tcgatggcgg
      181 gcttcaggaa attgagcagg ttgcgggcgt acatctcgct ggcgttgtaa gccagggtgc
      241 cggccacgtt caccggaccg atgatcttca ccccctggtg gatgacggtt tcgcccgcct
      301 tggtgagctc gcagttgccg ccctgttcgg cggcaatgtc gacgatcacc gagccggcgc
      361 gcatgcgctc cacggcggcc ttggagatga tcaccggcgc ctttcggccg gggaccgaag
      421 ccgtggagat gaccgcatcg gaagcggcaa tgcgggcatc gagcgcctcc tgctgcttgg
      481 ccttctcctc cgccgtgagc tcgcgggcat agccacctac gccgtcggca ctgaccccgg
      541 tctcgacgaa cttggcgccc agcgacttca cctgttcgcg ggtggcgctg cggacgtcat
      601 aggcctcgac cacggcgccc aggcgacgtg cggtggcaat ggcctgaagt ccggcgacgc
      661 ccgccccgat gatgagtgcc tgcgagggac ggatggtccc ggccgcggtg gtcagcatgg
      721 gcatgaattt ttcgaggttg ttggcggcga tgagcacggc cttgtagccg gccagcgaag
      781 cctgggagga caggccgtcc atcgactgcg cgcgggaaat acgcgggatc agctcgaccg
      841 caaaactcgt gatcttgcga tcgcgaaggg ctttgacctc ggcgtgtttc gtgtgggcct
      901 gaaggaaacc caccaccacg gagccgggct tgagctgggc aatctgctcc aggctgagcg
      961 gcgcgacggt cagcagcacg tcggactgcg agagtacgtc ggcggaactg gccagccatt
     1021 cacccttctt gtacagcgcg tccggaaact gggcgcggat cccggcgccc ggttcgagca
     1081 ctacacgtgc accggcggcc aggaatttgt cgaccacctc gggtacgagg ctgacgcgcg
     1141 tttcaccggg cgcgctctcg cgcagcgcgc ctatcgtaat cggcatttgg cttgactccc
     1201 ctgacaggcc tgtggcgatg gagccaaagg cctttttttc ccgtgttaaa tgaatgtctt
     1261 gcgacgttga attctcgcat acgactggtc tatcttacat gcctcatgtt gggcatcgat
     1321 cttgaaaagg cagatcgagg tgacggctcg gatctgcgcc tgctcgcgag cgacttgggt
     1381 cgcgcgctct cccaactcgt cctgcgaacg gacgtgtccg gcatgccgct cgagtggatc
     1441 gactacaagg aagcggcgcg cctgtatcac caggagcagg tcgcttatac ctgcggtagt
     1501 ccgctgttca agctttatgg cggtgttagc gctcgcacgg gttttcggac ggtgctcgag
     1561 gtcaactcga tcgtcgcgac ggttggtcac accggcaatc cgggcaacac ccgtcatgac
     1621 tacgttccgc cgctgaacaa ccagacgctg ttccggcgcg acgctcactt ctgcctgtat
     1681 tgcgcgcagc ggttcccggt gcgcgagctg tcacgagatc acatccggcc tttcagccaa
     1741 ggtgggcgtg acgtctggac gaacgtggtc gcggcctgtc gccgctgcaa caacctcaag
     1801 gcgtcgcgta cgcccgagca ggccaagatg cagctcatcg cggtgccgtt cacgccaacc
     1861 tacgcggagt acattttcct gaagggccgg cgtgtgctcg ccgaccagat ggaatacctg
     1921 cgcgcgcatt tcccgcggca ctcgccgctg cacgcgcgca tcaagcgcta cctgagctag
     1981 ccggggactg atcttttttt gcgcctcgca aaaatagatc tgtcccctat tcttgctact
     2041 gcgccgctac gcgcagccag aaccgcgcga cgacttcctc gtgctcggga gaatggccgc
     2101 gcacgatcaa atcgtagtcg ccggcatcga ggcttcccgg gtcgcgcaat gtgaatgcca
     2161 gctgcgtgcg gcccaccggt gcctgccgcg atgagcgcca cgcgagcgtc gcgccacggc
     2221 gattgaccag gtccatctcg tactcggcgt actgcggctg ctgtgggctg tggccaccca
     2281 cgcattcgag ttcgatcgcg ccgtcctcgc aggtcagcac gaccacgtcc ggtttgatga
     2341 tcagctcacc aggagcatcg gtgagcgaga cgttgatgtc ggggatgcta tccgcgccgc
     2401 ggacgttgct gaggcggaac gtgctggaag ctgcgacgcg ctgtacggag gatgcgaccc
     2461 ggttcgtgcc aggcgcctgg ctttcatcgc gcggcaggaa gacgccgagc cccaggccca
     2521 acgcaaccaa tacgctcgcg gcgagcgcga actgccactg gcgctcgcgc cgtggctcgg
     2581 gtgcgcttcg gttcagccac ccacgacgag ccgcgctggc gatgccacgc cgcaggcggc
     2641 gttccatctc gacctcggcg gcgagttcgg gggacgcgac cagccaggcc tcgaactcgg
     2701 cacgttcgga ccgggaaagg tcgcccgcga cataacgcga aacgagcctg gtctgctctt
     2761 ctagcgaatt gcgatcaaga gacttcatga gtgacttgtg tcaccaggag ccaaaatgtc
     2821 tcacccaaac tgtgaaagac atcatcagag gcagcaaaat tgaggagatt cgcggaaaaa
     2881 gggccaagtg gggcgcccgg cgttcaatga tggtgcgcag gcggctgcgc gcgcgcatca
     2941 gcacgcggtc gaaatgctta ggggaaagtc cgagttcgtg acatagctgc tgtttgtccg
     3001 tgccgtctag atagaaccgc gtcagcagct ggcggtcgcg ttcggtcggc agctcttcga
     3061 ggagctcagc cactgcctgg gccagatgtc gatcgtccga ttcggacagg tcgctctgct
     3121 ccatttcgag attctgcgca agctcgtcga cgagctccga atcgaccaat gtctgctggc
     3181 gtaacgcctt gcgcgccgcg ttgagcgcga ggttgcgcgc agtagccgca gcgaacgcaa
     3241 acaccttgcg cgggtcgtcg acggtcctgg cacgcacgcg ttcgacgacg atgtgcaggg
     3301 tttcctgcgt caggtcctcg gcgtcgatcc agcgatggaa ggcgccaaaa cgcagtaccg
     3361 caagcactcc ggggcgcagt gccaccagca tccgcgactc ggccagccgg tcgccggtca
     3421 gcgccgcggc aacgatgcgt tcgatttctt cgacggggat cgtgctcaca cgccacccgc
     3481 cacgcgctgg tagttagttg cactcgagcg ccgccatggc ggaccggcag tccgcgcccg
     3541 gatcgagatt tgcgagttca cgaaggaatt cgacattgac ccatggatac cgcgtggaat
     3601 tgctgtcctt actcctgcag aacaacagtt gttttatctg ctgggcgtcg cacttgttga
     3661 gcggcgggct tccccagacg agcgccactg caccagagac gtaggccgcc gcgctcgatg
     3721 cgcccgcgga ctcggacagt gcatagcctc gctcggaatt ctcggtcgtt gtcaccacga
     3781 tgtccacgcc gggtgcctga attcggccgt cgttcgcatc cggatggccc ctaggattga
     3841 acaattcgtc gccgcgcata tcggtcggga ctgcggcgat gacccctggc aggcagcggt
     3901 attgcgaggg gaaatactcg cggaccgtgt cgtcacggtc gaggctcgtg gcatagccgg
     3961 tgaccatcag ccgccttgcg gatgacgagc cgccaacggc ggcgatgatt gattccgaaa
     4021 tcgcgcggct gtcgaccggc catttcgcac tgatattgat gatttccgcc ccgtcgtcga
     4081 ccgcagcgtc gatgccggca gcgatggtgg agattcgggc cagcaggccg cggtcgacgc
     4141 ggctgatgac gattggaagc agcctgctgt tgggtgccac acccgccacg ccgattccat
     4201 tgtccatccg gccgccgatg gtgccggcca tttccgtgcc atgcgcataa caccggccac
     4261 tggtcgcgca acgcgcattc agcctcccgc cctgcgagcc cggtgtttcg tgtgcatgca
     4321 ggtgcaagac cacatttctg gccagatctt cgtgagcggc gtcgatgccc gaatcgagca
     4381 ccgcgacgat gcgaggcaag gaccgggcgg cgatgcctga atcccagccg atcttcctgt
     4441 gaccccacaa ctcatcaaga caaggatcgt tggggaacgt ggatcgcgcg gcgcgcaacc
     4501 agcaacgcga tgcggaaacc gccgcaggcg gaagctccat ggaagtggtg aggtagtcgg
     4561 gaccgtcgca gtcgggttcc gaataaccaa ccaaaggcga gttgatgatt gcgatcagtc
     4621 gttctgtcgg ggtgtatcgc gaaaagacga ccacgacgaa attgcgcgcg agctcaacgt
     4681 cccggacgta cccggtgtgc tcgagccagt cgacgagctg gcgctgactg gttccttcgg
     4741 cgggaaacac gatgaggcgg gcgggtacct gcctgttccg ctgcgcggcg ttagccgcgg
     4801 atcgatgacg ctgggaaaac atgcccgccc agtgcgcgca ggcggcgcgc aggtcgaagc
     4861 gcgcatcacc cagggcaccg agatattcga tgacggcaac aggcgcgcgg gccgcggtcg
     4921 gcgcccactg accgccgtga gcacagaggt tgggtgcggc cgccagcgca cacgacgctc
     4981 caaacgtcgt tgccgcggca agcggcaaca acatttttga gagtcgtggc gcaagcggac
     5041 gcaatttcga accgtgtact acttgatctg gatgatgccg ccatcagtca cgcgcgccgg
     5101 cgagtcgacc aggatcgaca tgacaggctt gccgttttcc tggaccagga aggcgatcat
     5161 gtcgttgagc tccaccgtgc ccttcacctt cgacgggcgc atggcgatgt cgcgcgagcg
     5221 caggcgatga caggacgaca gcacttcggc attgcccgcc ttcacgtcat cggccgtgca
     5281 aaaacgagtg ccgcgcgtga acaacatcgt cacgccgcac ggcgtggcga aatccatgtt
     5341 ccagggtgag cgctgcttgc tcgagtttcg cgtgcagatg cgcacgacat tttcctcggc
     5401 gtcgaaaaca aacgtcagct tgccgcccgc cggcatgtga tcgttgagcg tcgacgacgc
     5461 gaccgtgtat tccttgccca gccatccggc gagcacactg tcaggagcat tttcctgcgc
     5521 cgttgctagt gtggccccga acccgaggaa tgccgcggcc gtccagatcc cgagcttccg
     5581 tccatcaatt ttcagtttcc cgtacatgag acactcctca ttcaattcag ccatttgatt
     5641 gatgcaattc ccgcgggcat cacggaaatt cgttagcccg aactacgaaa ctcgaccaca
     5701 cggcgggcgc agcgctgcta cgagtacgca gctgcgcggt gcgcaacgcg tcggccgccc
     5761 ggccggagcc gcgatacgct tgataaaact cgttcatgaa gcgtgccgtt gacgcgtcct
     5821 cgatgggcca cagagtcgcc acgaccgaat gcgacccgtt ggcgagaaat ccgtacgtga
     5881 gcccgagcac gccttcaccg cgaagggcgc tgccgtcacc ggtcgcgcag cccgagagaa
     5941 ccaccacatc ggcgcgcagc ccactgcgcg agatttcgtt tgccgtgaga cgactgtccg
     6001 gcagctgcgc gccttccggt gtgtattcgc tgaggaaaag cgccgactgc tcgggtgaat
     6061 cctggcgtgc cacggcgtgg gtggcgaaat gcaggacggc aagttcgctc gacggcaatt
     6121 tcaacactcg ttcggcggtg gcgtcgaacc ccgacaactg gatcgtgtcg gcggatccga
     6181 acgccttcgt gacggcactg gcttcgagag ccgaataagg cagccgtgtg agattgttag
     6241 gcgacgcacg ccgcgcgcca cgcagggttc ccgcaccacc gccctgggcg attcgcaacc
     6301 ggcgatcgtc ggctgcgtaa accgggtcgg agatcaccgc cacgcgcgtg ttgtgcgact
     6361 tggccggacg ggccttctcc attgccagt
//
LOCUS       DASQJS010000125        11336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1635585, whole genome shotgun sequence.
ACCESSION   DASQJS010000125 DASQJS010000000
VERSION     DASQJS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11336
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635585"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2620)
                     /locus_tag="VEW08_05170"
     CDS             complement(<1..2620)
                     /locus_tag="VEW08_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ40159.1"
                     /translation="MNHSIKSPASSVAAGVRAALHGGGRAATATALGLSFVLSAPAFA
                     QTANEGGQGAASSEANDLGEIVVTGIRRQLETSQARKQDATELVDAITADDIGALPDR
                     SVTEVLQRIPGVAIGRVPQPRDADRIAIEGAGVTIRGLSWVRSELNGHTAFSAKNSRV
                     LGFEDVPPELLSGVDVYKNPSAQQIEGALSGTVNLRTWLPFDSEEHKISFSVQESWGD
                     LAEKWEPSGSALFSGRTETSFGEIGGLISASYSDLTARTDTLHIDKYYARTDLVPGQT
                     VYATGGIGWRQLLVDRNRTGVSAALQWQSPGESVEAHLQYFYSDATFEQDENAVWNTP
                     GGGLNGTGLTYSGDKLTGGTLNDGGYNGSARYNKREATNDDVSLHLTWSATDRLTFEA
                     DIQNSKATTKIIDLTMGPSAGPNFENAGPYSLQLNGSNEPTIVIPANNVLTNPNAVYH
                     GFAMDHHEDNDADAWAYRADAEYTFDNSDWLDKIRFGVRYEDYNSTTRETGYRWGSIS
                     QNWAGGPAIFAQQDVPYRQQDYSNWFHGGNAPSGFLFPDTGFFRNYQGWSEQVIAVST
                     APGVNNGCCGWTPFDGDYSTKFPANDGLGVNPQNQTTTAGYAQLSFGSGKWDGNVGVR
                     FVKTEASGSGQLVFTQGNFGPGADPDDVAFSNGAAVETSGTNTYDDVLPSFNLRYKAT
                     DNFFLRFAVGKGIARPDFPQLLPSININPQVGQITNGACLPLPPNVTVGDCVAGYNGF
                     AGNADLKPMESTNYDLSAEWYLNSTNSLTLALFDKEVSGFIETTLGVVVPYENNGVTR
                     DVNVLRPENQGDGFVRGAELAWNGFFDFLPGFGKHFGARAAFTYVDSGGTRNSAANPY
                     DPNQQVNSTAEDYPLEG"
     gene            complement(2828..3997)
                     /locus_tag="VEW08_05175"
     CDS             complement(2828..3997)
                     /locus_tag="VEW08_05175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ40160.1"
                     /translation="MSDTQAIDVIVIGAGIAGTSVAYFLSNHAKVVVLERESQAGLHS
                     TGRSAALFSETYGSSQVRALTRASRAFLERPPRGFVENPLLSPRGALVIGTQEQSAQI
                     ESDCAAMRVFTPQLQLLGEEQMRSLVPVLEPQFAQAGFFEPGAADIDVNSLHQGFIRG
                     LKQRGSRLECGVQIHSIERSGGEWLLEVAMRGAQPHSLRAPLLIDGAGAWADEVAALA
                     GVAPLGIEPRRRSAFLFQPPAGVATAHWPFVTSVGEDFYFKPDAGLLLGSPANADPVR
                     PHDVQAEELDIALAIDRIERATTMRIERPLRPWAGLRSFVSDGGLVGGFDPTARGFFW
                     VAALGGYGIQTCAAMGEACAHVVLGRPLPTQLSDAGLSTTMLGVERLRSSARAAI"
     gene            complement(4007..5230)
                     /locus_tag="VEW08_05180"
     CDS             complement(4007..5230)
                     /locus_tag="VEW08_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HYJ40161.1"
                     /translation="MSPTARVLTALLGGAALGIVLTGVQPALARDMAGVAQPIGDLWL
                     NALQMTVVPLVVALVVLGVASTSDAAASGRTARRAILVFLVLLCAAATFAAIYAPFQL
                     SLLPRDPEFIASLRAAIGTPPATDAAATGFAQRLVTIIPSNVIAAAAQSAMLPLVVCA
                     LLFGFALTRVEPRRRAQAIDLFQTLKDTTVTIVGWVLWAAPLGVFALAFSVCVKAGFS
                     VLSALGWYILAMCTLYLVVTLGLYIVAVLAGRQRLRQFAAGILPAQVIAVSTQSSLAT
                     LPAMVESAQNRLGCSPAVTALVLPMAVSLFRITSPVQYIGVSCFIAWIYGVDLTTSQL
                     AVGAALAVVISLGSVGLPGQVSLLAMNMPVVQAMGLPIAPLGLMLAVETIPDAFATLG
                     NVTGDLAATTVVARS"
     gene            complement(5241..6086)
                     /locus_tag="VEW08_05185"
     CDS             complement(5241..6086)
                     /locus_tag="VEW08_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HYJ40162.1"
                     /translation="MSSLVKIRAERDQMSAIERRIADFILENAHLLRDYSSQQLANSL
                     RISQSSVVKFSQKLGFKGYPDLKVSVSEAVARENGNGESRATPPSRSDPQSALAADLW
                     RSKTLAEEETRLINTPAKIAEIARALNVAGKVFVIGLGEDGVPARAFSMKLALLGIPV
                     ICHTDPVSLSAALAIAGKGDVLVLFSEHGRQPALGQIERQFHEQRGKVVSVTRHSANS
                     LRAHADMSLLVSAHDDRAHIQPLLYSAVLQHLLDLVYIMLCDESDRIDHLNASIKRAQ
                     RFNEP"
     gene            complement(6086..7183)
                     /locus_tag="VEW08_05190"
     CDS             complement(6086..7183)
                     /locus_tag="VEW08_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HYJ40163.1"
                     /translation="MKITDIRFGMLRVPLKTPFKTALRTVDTIEDVIITMHTDTGHIG
                     YGEAPATAVITGDTHGSIIEAIHKVIAPRIIGEEIANLNRITRLIQGALEKNSSAKAA
                     VEIAVYDLFAQLYGAPLYKILGGGDPVITTDITISVDYIDKMVADSLAAVERGFESLK
                     IKVGKDIELDVERVKAIYAAVEDRALIRVDANQAWTAKQAVFAIQTLENAGVRMELVE
                     QPVKANDLEGMKYVTERVHTPIMADESVFGPTEVIELIRMRAADIINIKLMKTGGISN
                     ALHIADIAAMYQIDCMIGCMVETSISVAAAAHIAVAKADVITKVDLDGPSLCQFNPVD
                     GGVLFNDSEITISDAPGLGIREIRGLEPIEV"
     gene            complement(7180..8559)
                     /locus_tag="VEW08_05195"
     CDS             complement(7180..8559)
                     /locus_tag="VEW08_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HYJ40164.1"
                     /translation="MSYALLAYLLASCSSQSPRTPAIPPHAVVGVQQEQLTPEFWIAR
                     ESNAKKIVLDAQAIALQNARLLQTDPSVHDIYAMPPALAGARVRSWIEGISSYPEKDM
                     FDAEGRKLERPDFDALMRNVALETVPESQPTRYGLVVKRADLRTFPAPLRGYNSADDR
                     DIDRFQESALFPGAPVIIVHQSRDGNWWFIVSSVYAAWVEKRFIAEGPAEAVLGYTRK
                     LPYLVVTGATAKTVFTPERPEVSELQLDMGVRVPVIADWPQARPVNGQHPYTAHVIEL
                     PERASDGTLKFTPALLPRTSDTSGDYLPLSRANLLRQGFKFLGERYGWGHSYNARDCS
                     GFVSDVYRSFGVQLPRNTRDQAVSPALNRITFTDADDHERRLAVLRNLETGDLVYIPG
                     HVMMVIGHDNGMPYVIHDTTGISYRDGTGEVARVQLNGVSVTPLTPLLFGRRDSIVEH
                     ITSILRVRP"
     gene            8749..10185
                     /locus_tag="VEW08_05200"
     CDS             8749..10185
                     /locus_tag="VEW08_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HYJ40165.1"
                     /translation="MTRVSCVVMMMLCSATRADTLEQVQKLVDAGQFKSAEGQITRSL
                     AQTSLSSADREALTFERERMRRIRLDFSLTADDAQSRLRRQIPDLTPAEFAAWDAAGL
                     LEHQVIDGQTLYFNRAPSNLFRLSEAAVKRRSSTTPPWTDGPMEAANAHHREVRDQAL
                     KTGKAGVAPRKVRVTYSLTVNADAVPGGETTRAWLPFPRVLPGQQENVRLIESSPAKH
                     VIAPESALQRTVYMEKLADAGKPTMFSITYELTVFGQYHLIDAAKIRPAARTPQLAPH
                     LAERAPHIVFTPAIREFSRKVVGTETNPFLIAQKLFAAVDRIPWAGAREYSTISNISD
                     YTLHAGHGDCGQQTLLLMTLLRLNGIPTRWQSGWVYSEGAYDNMHDWAQLYLPPYGWV
                     PMDVTFGRLASGDPQIDAFYLGGLDAYRIAFNDEFSQPFEPAKQHARSDTVDSQRGEV
                     EWRGGNLYFDQWDYKFEAQVLTVPQETG"
     gene            10210..>11336
                     /locus_tag="VEW08_05205"
     CDS             10210..>11336
                     /locus_tag="VEW08_05205"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ40166.1"
                     /translation="MFYKRFISARTPVLSCMALFIALLISVGTSQLVHAASGDGSLVG
                     HLNATDKNAVSGAEIAARNVDTGFTRKVKADADGSYRFPYLPVGTYTVEASKDGKSLG
                     SLDGVPVSLGVATRADLDLGDTTLETVTVVGSRVQNFVDVTSTESATNVTAEELERLP
                     VERDLMSVAQLAPGLSKGDSAFGGVSFGGSSVAENSVYINGLNVTDFYNRIGFSSVPY
                     AFYKEFQVKTGGYSVEYGRTTGGVINAVTRSGTNEFEFGAEMVWEPSSAQASGDNYYS
                     PDSTDADTLPQAYRILQYDEYDRMNATAYASGPIIKDKLFFFAIYEARNYEPVNTDNT
                     GANFFEAESDEGFWGAKIDWQISDKHLLELLAFSDENQKLS"
BASE COUNT         2163 a         3605 c         3481 g         2087 t
ORIGIN      
        1 acccctccag cggataatct tccgccgtcg agttgacctg ctggtttgga tcgtacgggt
       61 tggcggccga gttgcgcgtt ccgccgctat ctacataggt gaatgcagcg cgcgctccaa
      121 agtgcttgcc gaagcccggc aggaagtcga agaacccgtt ccacgcgagc tcggctccgc
      181 gcacgaagcc atcgccctgg ttttccgggc gcagcacgtt gacgtccctg gttaccccgt
      241 tgttttcgta aggcacgacg acacccagcg tggtctcgat gaatccggac acttccttgt
      301 cgaagagtgc cagcgtcagc gagttggtcg aattgaggta ccactcggca ctcaggtcgt
      361 agttggtgga ctccatcggc ttcaggtcag cgttacccgc gaaaccgttg tagccggcca
      421 cgcaatcgcc caccgtgaca ttcgggggta gtggcaggca ggcgccgttc gtgatctgac
      481 ccacctgggg attgatattg atcgacggca acaactgcgg gaaatcggga cgtgcaatgc
      541 ccttgcccac cgcgaatctc aggaagaagt tgtccgtggc cttgtaccgg aggttgaagc
      601 tcggcagcac gtcgtcgtag gtgttggttc cagaagtctc gacggctgcg ccatttgaga
      661 atgcgacgtc gtcgggatca gcaccgggcc cgaagtttcc ttgagtgaag accagctggc
      721 cggatcccga ggcctcggtc tttacgaacc gtacgcctac attgccgtcc catttgccgc
      781 tcccgaagga aagctgcgca taacccgcgg tcgtcgtctg attttgcgga ttgacgccca
      841 ggccgtcgtt cgcggggaat ttcgtggaat agtcgccgtc gaatggcgtc cagccgcagc
      901 aaccgttgtt cacgcccggt gcggtggaca cggcaatgac ctgctccgac cacccctggt
      961 agttgcggaa aaagcccgta tccgggaaca ggaaaccact cggcgcgttt ccaccgtgaa
     1021 accagttgga gtagtcctgc tggcggtacg gcacatcctg ctgggcgaaa attgcaggtc
     1081 caccggccca gttctggctg atcgagcccc agcggtatcc ggtctcacgc gtggtcgagt
     1141 tgtagtcttc gtagcgcacg ccgaatcgaa tcttgtccag ccagtcgctg ttgtcgaacg
     1201 tgtattccgc atcggcacga tacgcccagg catcggcatc gttgtcctcg tgatgatcca
     1261 ttgcgaatcc gtggtagacg gcattcggat tggtgaggac gttgttggcc ggaatcacga
     1321 tggtgggttc attggaaccg ttgagctgca gggagtacgg ccccgcattc tcgaagttcg
     1381 gcccggccga cggacccatg gtgaggtcga tgatcttcgt ggtggctttc gaattctgaa
     1441 tgtcggcttc gaaggtcagc cgatcagtgg cactccaggt gagatgcagc gacacatcgt
     1501 cgtttgtggc ttcgcgcttg ttgtagcgag ccgagccgtt gtagccaccg tcattgagcg
     1561 tgccgccggt cagcttgtct ccggaatacg tgagccccgt gccattcagg ccgccgcccg
     1621 gtgtgttcca cacggcgttt tcatcctgct cgaaggtcgc gtcggaatag aagtactgca
     1681 ggtgagcttc gacgctctcg cccggcgact gccactgcaa tgcagccgac acgcccgtcc
     1741 tgttgcggtc gacgagcagc tggcgccagc caatgccacc ggtcgcgtac acggtctgac
     1801 ccggcacgag gtcagtgcgc gcgtagtact tgtcgatgtg cagcgtgtcg gtcctggcgg
     1861 tgaggtcgga gtagctggcg gaaatgaggc caccgatttc gccgaacgaa gtctcggtcc
     1921 tgccgctgaa caatgccgat cccgaaggct cccacttttc agccaggtcg ccccaggatt
     1981 cctggaccga gaagctgatc ttgtgttctt cagaatcgaa tggcagccag gtacgcaggt
     2041 tcacggtgcc ggacagggcg ccttcgatct gctgagcgga agggttcttg taaacatcca
     2101 cccctgagag caactcggga ggaacgtcct cgaagccgag gacgcggctg tttttcgccg
     2161 agaacgcggt gtggccattg agctcgctgc gcacccagct caatccacga atggtgacgc
     2221 cggcgccttc gatggcgatg cggtccgcgt cacgcggttg cggcactcga ccgatagcca
     2281 cgccgggaat tcgttgcaac acttcagtga cgctgcgatc gggaagcgcg ccgatatcgt
     2341 cggcggtgat cgcatcgacc agctcggtgg cgtcctgctt gcgggcctga gacgtttcga
     2401 gctggcggcg aatgccggta acgacgattt caccgagatc gttcgcctcg gaactcgctg
     2461 ctccttgccc accttcgttc gccgtctgcg cgaatgccgg cgctgacaac acgaagctca
     2521 aaccgagcgc agtggccgtg gcagcccgtc cacctccatg cagcgcggcg cgtacgccag
     2581 cggcaacact cgaagcaggc gatttaattg aatgattcac agtgaccccc cgttgaagac
     2641 tgaaattggt gaatcgcagc gccatcgacc tccgtcgagc tggccccccg ctgcaccaat
     2701 gaaatccttt ttattagcga taccgtaccg cagaaattcc gtttcgtcca cacgggcgct
     2761 gtcccggatt tggtatccgg ggtttcgtta tcgacacata tctatagagg gggcgggcgg
     2821 gcagtttcta gattgctgct cgagcgctgc ttcgaagtcg ttccactccg agcatggttg
     2881 tcgacaagcc ggcgtcactc agttgtgttg ggagtggacg acccaacacc acgtgcgcac
     2941 aggcctcgcc catcgccgca cacgtttgta tgccgtagcc gccaagcgca gccacccaga
     3001 agaaacctcg cgccgtgggg tcgaaacccc cgacgagccc gccatcggac acgaaggaac
     3061 gcaggcccgc ccaaggacgt agcggacgtt cgatccgcat cgtcgtcgca cgctcgatgc
     3121 gatcgatcgc caatgcaatg tcgagttctt cggcctggac gtcgtgcggg cgcacggggt
     3181 ccgcattggc gggggacccg agcaggagtc cggcgtcggg tttgaagtag aagtcttcgc
     3241 cgacgctggt cacgaacggc caatgcgccg tggccacgcc tgcgggtggc tgaaacagga
     3301 aagccgagcg gcgccgaggc tcgataccca gaggcgcgac ccccgccagt gcggccactt
     3361 catcggccca ggcacccgcg ccgtcgatga gcaatggtgc ccgcagggaa tgtggttgcg
     3421 caccgcgcat ggcgacctcg agaagccact cccctcccga gcgttcgatg gaatgaatct
     3481 gtacgccgca ctcgaggcgg ctgccgcgct gcttcaggcc gcgaatgaaa ccctgatgca
     3541 gtgaattgac gtcgatgtcc gccgcaccgg gttcgaaaaa ccctgcctgc gcgaattgcg
     3601 gctcgagcac cggcaccagc gaccgcatct gctcctcgcc gagcagttgc aactgcggcg
     3661 tgaacacgcg catggccgcg cagtcacttt caatctgcgc ggactgttcc tgcgtgccaa
     3721 tcacgagtgc gccgcgaggg ctgagcaacg gattctcgac gaagccgcgc ggcggccgct
     3781 cgaggaacgc gcggctcgca cgcgtcaatg cgcgtacttg cgaagaacca taggtttcgc
     3841 tgaacagcgc cgccgagcgg cccgtcgaat gcaggccggc ctgcgattcc cgctccagca
     3901 cgaccacctt cgcgtggttc gagaggaaat acgccacgga ggtgccggcg attccggctc
     3961 cgatgacgat gacgtcgatt gcctgagtgt cactcatgcc cggagctcat gaccgcgcaa
     4021 cgaccgtggt cgccgccaga tcgcccgtga cattgccgag cgtggcaaac gcatcgggaa
     4081 tcgtctccac ggcaagcatc aggcccagcg gagcgatcgg cagccccatc gcctgcacca
     4141 ccggcatgtt catcgccaac agactcacct ggcccggaag cccgaccgaa cccaggctga
     4201 tcacgaccgc gagcgcggca ccgacggcca gctggcttgt ggtcagatcg acgccataga
     4261 tccacgcgat gaagcaggac acgccgatgt attgcaccgg gctcgtgatc cggaacaacg
     4321 acaccgccat cggcagaacg agcgccgtga cggccggcga gcatcccaac cggttctgcg
     4381 cgctttcgac catcgccggc agtgtcgcca gcgaagactg cgtgctcacg gcgatcactt
     4441 gcgcgggcag gatccctgcc gcgaattgcc gcaggcgttg cctgcccgcg agaacggcga
     4501 cgatgtacaa cccgagcgtc acgaccagat agagcgtgca catcgccagg atgtaccacc
     4561 ccagtgcgct cagcacactg aatcccgcct tcacacaaac gctgaacgcg agcgcgaaca
     4621 cgcccagcgg cgccgcccac aacacccagc caacaatggt gaccgtggtg tccttgagcg
     4681 tctgaaacaa gtcgatcgcc tgagcccggc ggcgtggctc gacacgagtc agcgcgaagc
     4741 cgaacagcag cgcgcacacg accagcggca acatcgcgct ttgcgcggca gccgcgatga
     4801 cattgctggg gatgatcgtc accagtcgtt gggcaaaccc ggtcgccgct gcatccgttg
     4861 ccggcggcgt tccgatggcc gcccgtagcg acgcgatgaa ctctggatcg cgcggcagca
     4921 gcgagagctg gaatggcgca tagatcgccg cgaaagtcgc cgcggcgcaa agcagcacga
     4981 ggaataccag gatggcccga cgcgccgtgc ggcctgacgc cgccgcatcg ctcgtggaag
     5041 cgacgccgag aaccacgagc gcaacgacca gcggcaccac cgtcatctgc agcgcgttga
     5101 gccacagatc gccgatcggc tgcgccacac ccgccatgtc ccgggcaaga gcgggttgaa
     5161 caccggtgag cacgatgccc aacgcggcgc cgccgagcaa agccgtcagt acgcgtgcgg
     5221 tgggagacat cgcgacctgg tcaaggttcg ttgaagcgtt gcgcacgctt gatgctggcg
     5281 ttcaggtgat caattcgatc gctttcatcg cagagcatga tgtaaacgag gtccaacagg
     5341 tgttgcagaa cggccgagta cagcagtggc tggatgtgcg cccggtcgtc atgcgcggag
     5401 acgagcagcg acatatctgc atgggcgcgc aatgaatttg cgctgtgccg ggtcaccgaa
     5461 acgaccttgc cccgctgctc gtggaactgg cgctcgatct ggcccagcgc gggctgccgg
     5521 ccatgctccg aaaacaggac cagcacgtcg cccttcccgg cgatcgccag tgcggcgctc
     5581 agcgacaccg ggtcggtgtg acagatcacg ggaattccca gcagcgcgag tttcatcgag
     5641 aacgcgcgcg cgggcacgcc atcctcgcct agcccgatga cgaatacctt gccagccaca
     5701 ttcaacgcgc gcgcgatctc cgcgatcttc gccggcgtat tgatgaggcg cgtttcctcc
     5761 tcggcgagcg tcttgctgcg ccacaggtcc gcggccagtg ccgactgcgg gtcgctgcgc
     5821 gacggcggcg tcgcgcgcga ttccccgttg ccgttctcgc gcgccaccgc ctcgctgacc
     5881 gacaccttca ggtcgggata acctttgaag cctaacttct ggctgaactt caccacgctc
     5941 gactggctga tgcgcaggga gttggcgagc tgttgggatg aatagtcccg cagcaggtga
     6001 gcattctcga ggatgaaatc cgcaatgcgt cgctcgatcg cagacatctg gtcacgctca
     6061 gcccggatct tgacgagcga ggacatcaga cttcgatcgg ctcgagcccg cgtatctcgc
     6121 ggataccgag gcccggcgca tcgctgatgg tgatttcgga gtcgttgaac agcacgccgc
     6181 cgtcgacggg attgaactgg cacagcgaag gcccgtcgag atccaccttc gtgatcacgt
     6241 cggccttcgc gacggcgatg tgcgccgccg ccgccacgct gatgctggtt tcgaccatgc
     6301 atccgatcat gcaatcgatc tggtacatcg ccgcgatgtc cgcgatgtgc agggcattcg
     6361 agatgccgcc cgtcttcatg agcttgatat tgatgatgtc cgccgcgcgc atgcggatga
     6421 gctcgatgac ctcggtcggt ccgaaaacgc tctcgtccgc catgatcggc gtgtgtacgc
     6481 gctcagtgac gtatttcatc ccttcgagat cgttcgcctt caccggctgc tcgaccagct
     6541 ccatgcgcac gcccgcattc tcgagcgtct ggatggcaaa caccgcctgt ttcgcagtcc
     6601 acgcctggtt cgcatccacg cggatgagcg ccctgtcctc gactgccgca tagatggcct
     6661 tgacgcgctc gacgtcgagc tcgatgtcct tgcccacctt gatcttcagg gattcgaagc
     6721 cgcgttcgac cgcggcgagc gagtccgcga ccatcttgtc gatgtaatcg acgctgatcg
     6781 tgatgtccgt ggtgatgacc ggatcgccgc cgcccaggat cttgtagagc ggcgccccgt
     6841 acagctgcgc gaacaggtcg tacaccgcga tctcgactgc ggccttggcg cttgagtttt
     6901 tttcgagggc tccctggatg agccgcgtga tgcgattcag attcgcaatc tcctccccga
     6961 tgatccgcgg cgcgatgacc ttgtgaatgg cttcaataat agagccatgt gtgtcgccgg
     7021 tgattacagc agtggcgggc gcctccccgt agccgatgtg cccggtgtcg gtgtgcatcg
     7081 tgatgatgac gtcttcgatc gtatccacgg ttcgcaaggc ggtcttgaat ggcgtcttga
     7141 gcgggacgcg cagcatgccg aaccggatat ccgtaatttt catggacgca ctcgcaaaat
     7201 gcttgttatg tgttcaacga ttgaatcgcg ccggccgaac agcagcggcg tgagcggggt
     7261 gaccgagact ccattgagct gcactcgggc cacctctccc gttccgtcgc gatagctgat
     7321 gccagtcgtg tcgtggatca cgtatggcat tccgttgtcg tgcccgatca ccatcatgac
     7381 gtgcccgggg atatagacga gatcgccggt ctcgaggttg cgcagcaccg cgagccggcg
     7441 ctcatgatca tcggcgtccg tgaaggtgat gcggttgagc gccggggaca ccgcctggtc
     7501 gcgcgtgttc cttggcaact ggaccccgaa gctgcgatag acatcggaca cgaagccgct
     7561 gcagtcgcgt gcgttgtacg aatggcccca cccataacgc tcaccgagga acttgaagcc
     7621 ctggcgcagc aggttggcac gactgagcgg tagatagtca cccgaggtgt ccgaggtgcg
     7681 gggcaataac gccggcgtga atttcagcgt accgtcgctg gcgcgctcgg gcagctcgat
     7741 gacgtgtgcc gtatagggat gctgaccgtt caccggcctg gcttgcggcc agtccgcaat
     7801 cacgggaacc cgcactccca tgtcgagctg caactccgaa acttcggggc gctccggcgt
     7861 gaagacggtt ttcgcggtgg cgcccgtcac gaccaggtac ggcaacttgc gtgtgtaacc
     7921 cagcactgct tccgccggac cttcggcgat aaaacgcttc tccacccagg ccgcatacac
     7981 gctgctcacg atgaaccacc agttcccgtc gcgactctgg tgaacgatga tcacgggcgc
     8041 gcccggaaac aatgcgcttt cctggaagcg atcgatgtcg cggtcgtcag ccgagttgta
     8101 cccgcgcagc ggcgcaggaa aggtgcgcag atccgcgcgc ttcacgacga gaccgtaacg
     8161 ggtgggctga ctctccggca ccgtctccag cgcaacgttg cgcatcagcg catcgaaatc
     8221 cggccgttcg agcttgcgcc cttcggcgtc gaacatgtcc ttctccggat aactcgaaat
     8281 cccttctatc caggagcgaa cccgtgcacc agccagtgcc ggcggcatgg cgtagatgtc
     8341 gtgaaccgac ggatcagtct ggagaaggcg cgcgttttgc aaggcaatcg cctgcgcgtc
     8401 gagcacgatc tttttcgcat tcgactcgcg cgcgatccag aactcgggcg tgagttgttc
     8461 ctgctgcacg ccgacgacgg catgcggagg aatggcggga gttcgcggac tttgactcga
     8521 gcacgaggcg agaaggtacg ccagtaacgc gtaactcaat tgcctcccaa gcgcgcactt
     8581 cattcgtcaa tgccccattt tgttccgagg aatagtctct tcccgcaaca ataaaatcaa
     8641 gaataaatta ttgacggtcg ccgcgaggcc atgttacaca gcactgaaca aggcggcgtg
     8701 tgcacagtga aagacgcgca attatcaaga ccgtcgactc tccagcaaat gacgcgagtc
     8761 agctgcgtgg tgatgatgat gctctgctcc gcaacgcggg ccgacaccct ggaacaggtg
     8821 cagaagctcg tggatgcggg ccagttcaag tccgccgaag ggcagatcac gaggtcgctc
     8881 gcgcaaactt ccctctcttc cgcggatcgc gaggcgctga ccttcgagcg cgaacgcatg
     8941 cggcgcatcc ggctcgactt ttccctcacg gccgacgacg cacagtcgcg tctccggcgg
     9001 cagattccgg acctcacccc cgcggaattt gccgcctggg acgcggcggg gctcctcgag
     9061 caccaggtca tcgacgggca gacgctctat ttcaatcgcg cgccttccaa tctctttcgg
     9121 ctgagcgaag ccgccgtcaa acgccgcagc agcacaacgc cgccgtggac cgatggaccc
     9181 atggaagccg ccaacgcgca tcaccgcgag gtccgcgacc aggcgctgaa aacgggcaag
     9241 gccggcgtcg cgccccgcaa ggtacgggtg acgtattcgc tgaccgtgaa tgcggatgcc
     9301 gttccgggcg gcgagacgac gcgcgcgtgg ctgccgtttc cgcgcgtgct gccaggccag
     9361 caggaaaacg tgcgcctcat cgagagctcg cctgcgaaac acgtcatcgc gccggaatcg
     9421 gccctgcagc gcaccgtgta catggagaaa ctcgccgacg ccggcaaacc cacgatgttt
     9481 tcgatcacgt atgagctgac ggtctttggc caatatcacc tgatcgatgc cgcgaagatc
     9541 cggccggcag cgcgcactcc gcagctcgcg ccccacctgg cggagcgcgc tccccacatc
     9601 gtgttcacac ccgcgatccg cgagttttcc cggaaggtcg tcggcacgga aaccaatcca
     9661 ttcctcatcg cgcaaaagtt gttcgcggcg gtcgatcgca ttccgtgggc cggcgcgcgc
     9721 gaatactcca cgatctccaa catcagcgac tacacgctgc acgccgggca cggcgattgc
     9781 gggcaacaga cgttgctgtt gatgaccctg ctgcggctga acggcatccc cacgcgctgg
     9841 caatcgggct gggtctactc cgagggtgcc tacgacaaca tgcacgattg ggcgcagctc
     9901 tatctaccgc cctacggctg ggtgcccatg gatgtgacct ttgggcgcct cgcatccggc
     9961 gatccgcaga tcgatgcgtt ctatctcggc ggactcgacg cctaccgcat cgcgttcaac
    10021 gacgaattca gccagccgtt cgagccggcc aagcagcacg cccgatccga caccgtggat
    10081 tcacagcgcg gtgaagtcga atggcgcggc ggcaatcttt atttcgatca gtgggattac
    10141 aagttcgaag cgcaggtgct cacggtcccg caggagaccg gctgagcgtt catgtcctag
    10201 ggagacaata tgttttacaa gcgattcatc agcgcccgca cgccggttct ctcgtgcatg
    10261 gcgctcttca ttgccctact gataagcgta gggacgtcgc aactcgtgca tgccgccagc
    10321 ggtgacggct cactcgttgg acatctcaat gccaccgaca agaatgccgt gagcggcgcg
    10381 gaaatcgcgg cgcgcaacgt cgataccggt ttcactcgca aggtgaaggc cgatgcggac
    10441 ggaagctacc ggttccctta cctgccggtg ggcacgtaca ccgtggaagc ttcgaaggac
    10501 ggcaagagcc tcggctcact cgacggcgtg ccggtctcac tgggcgtcgc gaccagggcg
    10561 gacctcgacc tcggcgacac gacgctcgaa accgtgactg tcgtgggatc gcgagtacag
    10621 aacttcgtcg acgtcacgtc caccgagagc gcgaccaacg tgacggcgga agaactggag
    10681 cgcctgcccg tggagcgcga cctgatgtcc gtggcgcaac tcgctccagg actgagcaag
    10741 ggagattccg cctttggcgg cgtctcgttc ggtgggtcgt cggttgccga gaactcggtc
    10801 tatatcaatg gactcaacgt cacggacttc tacaaccgca tcgggttctc gtcggtgccg
    10861 tacgcgttct acaaggaatt ccaggtcaag accggcggct actcggtaga gtacggtcgc
    10921 accaccggcg gcgtgatcaa cgccgtgacc cgctccggca ccaacgaatt cgaattcggg
    10981 gcggaaatgg tgtgggagcc gagctctgcg caggccagcg gcgacaacta ctactcgccg
    11041 gatagcaccg atgccgacac gcttccgcag gcgtaccgga tcctgcagta cgacgaatac
    11101 gaccgcatga acgcgacggc gtatgcatcg ggtcccatca tcaaggacaa gctgttcttc
    11161 ttcgcgatct acgaggcgcg caattacgag cccgtcaata ccgacaacac cggagcgaat
    11221 ttcttcgagg ccgaatcgga cgagggcttc tggggcgcga agatcgactg gcagatttcc
    11281 gacaagcacc tcctcgagct gctggcgttc tccgatgaaa accagaagct gagcga
//
LOCUS       DASQJS010000126        11765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1321536, whole genome shotgun sequence.
ACCESSION   DASQJS010000126 DASQJS010000000
VERSION     DASQJS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11765
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321536"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VEW08_05210"
     CDS             complement(<1..871)
                     /locus_tag="VEW08_05210"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40167.1"
                     /translation="MTPQLAAYCEQLLTRVRESGALALFEASAPELRDALPRVLAGSD
                     FVCNSLARDAGIAKWLIDGAELTRPLSHGEMERRLAAVIAEPDLAGFMAALRRQRERE
                     MVRIAWRDLAGWATLNETLADTSAFADAAIQAAVEYASQDLVRTYGEPLNTAGERQEF
                     IVLGMGKLGGGELNFSSDIDLIFLFPDKGTTAGARCIDNEDYFTRLGRLVIRLLGEGT
                     AEGSVFRVDMRLRPFGDSGPLTASGMFLDNYLQTHGRDWERYAWIKARAVTGTAAYKL
                     IQAESVRPFVYRRY"
     gene            968..1843
                     /locus_tag="VEW08_05215"
     CDS             968..1843
                     /locus_tag="VEW08_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40168.1"
                     /translation="MKMRLCSLVAAGGLVLVSACARQESAREIDWAKAALARNPAYEI
                     VATDDSAGVFTVRNTTTGQVETLKLHDLIAAPLPPRVTAQAAPAPAPAPAAPAEPVES
                     PEVEDDSGTLASRQTTEPEAGAALANGALAQGPGYSIQRGDVAAAPAAGTIEGPGYSI
                     TRQEPTTAPSNSGSEALASNVERRTDPIVCQGDRLMRIDGETMEFAGDAVIAEKGCDL
                     YISNARISAGGVGIIARQARVHIVNSTIGGTRGSYEASEGAEIYVARSTFTGIGRRFD
                     TATMNDLGGNAYEPN"
     gene            1863..2936
                     /locus_tag="VEW08_05220"
     CDS             1863..2936
                     /locus_tag="VEW08_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40169.1"
                     /translation="MRAFAIVVLASASLAAAADPGNSAALEQYRKLRCEPRSLSVQSV
                     EPLRFAATATCYKLPKSFAHDGWVQLVAVDVGAGTVRLVDLGRDNDAARTLQHDGHGG
                     LRWIARRRPHADQSRELEVWTLAASETQPRLTGQLELPFDVGNGLDATRGTQCMLIGG
                     SPQNHVLVKFIDGGLSVQPVAGLRGVRLWHSAHQAFVVQTDPESLAVHALLDCDGQTR
                     PLPEGDAARFAEFPREQILISSRSDWLVRIESSEGEGTELVIFQGDSRRSFGPFEWMP
                     KGCPDICVAEGLQLMDPGWSPGGGVFIAHEFFEAMIVSGEKLTRIASWNYRDNGAANH
                     TALLSDSLAMQLSKKRGVIFHRW"
     gene            2968..3699
                     /locus_tag="VEW08_05225"
     CDS             2968..3699
                     /locus_tag="VEW08_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HYJ40170.1"
                     /translation="MTYCVGVQVDQGLVFLSDSRTNAGVDQVSTFRKMTFFEMPGERV
                     IVLLAAGNLAVSQSVTGLLRDRVTQARNSLLTTRTMADAARLVGDAVRDVYHRDAEPL
                     REHGIEFNPTFIIGGQIGGEAPRLFHVYSAGNFIETTADTRYFQIGESKYGKPIIDRV
                     VKADTGLAAVAKCALISMDSTIRSNLSVGMPLDLAIVRRDALAISLHRSIGPDDAYFK
                     VIRDGWGESLRNAFHALPDPSIPQS"
     gene            3788..4792
                     /locus_tag="VEW08_05230"
     CDS             3788..4792
                     /locus_tag="VEW08_05230"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HYJ40171.1"
                     /translation="MRDPPTSRRTALQALLALGGSLTVRDLFAEPNIGTRLPGTPEDL
                     SGALADPEPLFAAPTRLDRIGRVMTHVKVNGKGPFRFVIDTGASRSTLAPHLARALAL
                     KPSVGRNVMLNGVTGAAEVPTVAVDSIEIGALRFEKQDLPVIFTSIMGNADGILGVAG
                     FQDQRIDVDFKRDRVSVLESNGKRPHYSMVTARATRNVNGLMIVDVRVGRRIRAKAVI
                     DTGAERSLGNLALQGAMNKNRKKRQVVSAVVHGATPDIADGDVQTIREATIGDMTLTN
                     LEVIFADFHVFKLWGLDQEPALLIGMDMLGVLERLVIDYRRNEVSMYGERATSRIVQR
                     "
     gene            complement(4804..5913)
                     /locus_tag="VEW08_05235"
     CDS             complement(4804..5913)
                     /locus_tag="VEW08_05235"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HYJ40172.1"
                     /translation="MASALKDQFGPSAPRAIAQMIRAVHADFPHDEFLRDALAGFAAM
                     TLTGRGFHIAAALRKHLPADYPQAIDVLLASASQPHAHRASGGMAAFLYMPHLFFVAR
                     HGLDHFEDSMRAQHALTQRFTAEYSIRAFLEKHPDATLARLREWTRDASPHVRRLVSE
                     GTRPRLPWAPRLRAFQKDPRPVLELLELLKDDSELYVRRSVANNLNDIGKDHPALVVT
                     VAKRWLRGATVERRWIVNHALRSAVKRADLGALGALGYGSKAEVALRDKRVTPPRVKI
                     GGAVTLSFTLVNKQQKPQRVMVDLVVHFVKARGTGAKTFKMKALNLAPGARVAVAKKI
                     GLRQLTTRKHYPGVHKVEALLNGRRVALGAFTLLR"
     gene            complement(5918..6547)
                     /locus_tag="VEW08_05240"
     CDS             complement(5918..6547)
                     /locus_tag="VEW08_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HYJ40173.1"
                     /translation="MLGIHNYWLFIATAILLVITPGQDTFFILGRSLAGGRSEGISAA
                     LGITAGSVIHTFAAALGLSALLATSPYAFMAVKFAGAAYLIYIGVRALLSRASGLPGD
                     EVRSDSGRWPAFRQGIFSNLLNPKVALFFLALMPQFIDAGSSRKVGAFLVLGLTFVTL
                     GVIWCLVLAIAAAKLRVAFLRRPSMANVLNKVAGAMFIALGLKLATARQ"
     gene            complement(6595..7179)
                     /locus_tag="VEW08_05245"
     CDS             complement(6595..7179)
                     /locus_tag="VEW08_05245"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HYJ40174.1"
                     /translation="MSFLDTRLFGNVLVARRTFLGVGGMGLSGAAVALLAGRDALAAA
                     AEKGANPASDVAILNTALAAELEAIAAYGVGAGSGLLKGAVLNLATTFQGHHKQHADV
                     LAGTVTKLGGTAAVAKAQYNFPVETLKTQNDVLRFAAGLEKGAVSAYLGAVPLFKDRE
                     LAKAAASILGDEAMHWAVLRQALGEEPVPAAFVS"
     gene            7310..8749
                     /locus_tag="VEW08_05250"
     CDS             7310..8749
                     /locus_tag="VEW08_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYJ40175.1"
                     /translation="MLSILVCHSYFLRFDQKQVKRAKPYPPLATLQVAAMLRNAGHEV
                     SFFDSMLADDLTRFERQLDVLRPQLVVFYEDNFNFLSKMCLGRMREACAEMIGLARAR
                     GVRLIAGGSDASDAPGSYLAAGADAVLHGEGLGALVALVDRINGDIHLPAHALAAGLA
                     DVSVAGISGPERGRAAGPLPEPAQASLPAWDLVDVEKYRRVWKKAHGHFSLNMASSRG
                     CSFRCNWCAKPIWGNQYVQRSPRETAAEMIYLRRHYRPDHIWFADDIFGFKAAWVNEF
                     AAALAEGGGGVPFTIQLRADLVSARMTEALRAAGCREVWIGAESGSQKILDAMNKGTT
                     VGETLQARKLLGAAGIRVGLFLQLGYLGEGLDDILATRKLVDEARPDDIGVSVSYPLP
                     GTKFYEQVKEQLGDKRHWRESDDLEMMFAGTYTSDFYRQLRNLLHDQVSHAGDRTLQQ
                     RWSDLIEREYEYRRNRQPEFGGGLSVAHA"
     gene            8742..10124
                     /locus_tag="VEW08_05255"
     CDS             8742..10124
                     /locus_tag="VEW08_05255"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYJ40176.1"
                     /translation="MPDILLTHGYFLAEDEKERQIMKPYPPLGLLYLSAFLKGAGFSV
                     EVFDSTFEEFSALEARMAATPGGVVGIYTNLMTRRNVLRIIQSARQHRWTVVLGGPES
                     ANYIPEYLDAGADVIVSGEGEITLTELLRTLPQQGVHRLQGVHGIAFRDESGTLVSTP
                     PRLRVPDIDSLPFPDREAIDHGRYLNAWKTHHGASSINLITARGCAYKCNWCSHAVYG
                     HSHRRRSPANVADEMQWIIDRYDPDQVWYADDVFTISHPWLASYTAELKRRGIHRPFE
                     TITRADRLQNQGAAALLRELGCYRIWIGSESGSQKILDAMQRGVKVDQVRLACRLAHE
                     QGIQVGMFLMWGYEGEEIEDIAATVEHVKQSNPDVFFTTVSYPIKGTGYFDKVRDRVE
                     MPIPWAEASDRDYVIAGRRGKDYYQLADVWLRNEVEAFRLESADPARAAELWGRAQQA
                     RESLQSWKVA"
     gene            10121..11347
                     /locus_tag="VEW08_05260"
     CDS             10121..11347
                     /locus_tag="VEW08_05260"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYJ40177.1"
                     /translation="MSNLHPDLPPFAVVEAALHKTTAFLAREFSAPSEAAPDWNEFEW
                     DVASSVSAMQGISALLAGRLKWVGPPAWQSFLADQAHHTRVRDERFGKLLATLEREMN
                     AAGIPILPLKGSALRDLQVHAPGVRPMSDIDLLVSPRDVGDSIMVLRRIGYVAKFSTH
                     RHLVLAPPDMRIPQCLGEHCDAPYKIELHTAIAEALPFTKVDITDRIRAQTQRAGINR
                     YPSAQALMCHLLLHAAGNMRANALRLMQLIDIAMLARRFGPADWLALLDTSPPAHAWW
                     MYPPLAMTARYLPGEIPPAALNMAYDICPRQLRRKAARHTLETVSWSNLRIAALPGYE
                     WSRNALELMRFARSRILPRRESTNELADAVNHQPDLLRIPWYQQSQANRIVRWLVSRP
                     PRVQTIMTLLAGQTPR"
     gene            11344..>11765
                     /locus_tag="VEW08_05265"
     CDS             11344..>11765
                     /locus_tag="VEW08_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40178.1"
                     /translation="MTLRDDEPPVSCEPWRSSSAPRRILAMRFQALGDTIITLPYLLS
                     LKRRFPEARLHFLTRKEVCQVPAAVDLFDRVIALGGGRNLKLQLLSAAWWMPWLLAQR
                     YDLVLDLQNHRISRWIRKLIAPRAWAEFDCYSPRSAGER"
BASE COUNT         2138 a         3941 c         3673 g         2013 t
ORIGIN      
        1 ggtatcgccg gtagacgaat ggccgcacgc tctcggcctg gatcaacttg taggccgcgg
       61 tgcctgtgac ggcgcgcgcc ttgatccacg cataacgctc ccagtcgcgg ccatgcgtct
      121 gcagatagtt gtcgaggaac atcccgctgg cggtcaacgg cccgctgtcg ccaaaagggc
      181 gcaaccgcat gtcgacacgg aacacgctgc cctcggcggt gccctcgccc agcagccgga
      241 tcacgagcct tcccaggcgc gtgaagtaat cctcgttgtc gatgcagcgc gcgcctgcgg
      301 tggtgccctt gtcgggaaac aggaagatca gatcgatgtc ggaagaaaaa ttgagctcgc
      361 cgccgccgag cttgcccatg ccgagcacga tgaactcctg gcgctcgccc gccgtgttga
      421 gcggttcccc gtaggtgcgt accagatcct ggctggcata ttccacggcc gcctggatgg
      481 ccgcgtccgc gaacgcgctg gtgtccgcca gcgtctcgtt caacgtggcc cagccagcaa
      541 gatcgcgcca agcgattcgc accatctcgc gttcccgctg gcggcgcagc gccgccatga
      601 accccgcgag gtccggctcg gcgatcaccg cggccaggcg tcgttccatc tccccatgcg
      661 aaagcggccg cgtcagctcc gcaccgtcga tgagccattt ggcgatgccg gcgtcgcgcg
      721 ccaggctgtt gcacacgaag tcgctgcccg cgagcacgcg cggcaacgcg tcacgcagct
      781 cgggagccga ggcctcgaat aacgcgagcg cgcccgattc gcggacgcgt gtcagtagct
      841 gctcacagta ggcggcgagt tgcggtgtca ccggcgcgga ctgtactaga aatatccgcc
      901 agaaaggccc accagaaaaa tcgccaatac cccttgcgga tggcgtgcag aggcctacga
      961 ttcgctcatg aaaatgcgtc tctgctcgtt agtcgctgcc ggtggtttgg tcctcgtcag
     1021 cgcctgcgcc cgccaggaaa gcgcgcgcga gatcgactgg gccaaggcgg cgctggcgcg
     1081 caatcccgct tacgaaatcg tcgccaccga cgactccgcc ggggttttca cggttcgcaa
     1141 caccacgacc ggccaggtcg agactctcaa gctgcacgat ctgatcgccg cgccgttgcc
     1201 gcccagggtc accgcgcagg ccgcgcccgc gcccgcgccc gcgccggccg cacccgccga
     1261 gccggtcgaa tctccagagg tcgaagacga ctccggcacg ctggcgtcgc gccaaactac
     1321 cgagcccgag gccggcgccg ctctcgcgaa tggcgcactt gcgcagggtc cgggctacag
     1381 cattcagcgc ggcgacgtcg cggcggcgcc ggccgccgga accatcgaag gtccgggcta
     1441 ctccatcacg cgccaggagc caaccacggc tccatcaaac tccggctccg aggcgttggc
     1501 gtccaatgtc gagcgccgca ctgatcccat cgtctgccag ggcgatcgcc tcatgcgaat
     1561 cgacggcgag accatggagt ttgcgggcga cgccgtcatc gccgaaaaag gctgcgacct
     1621 ctacatcagc aacgctcgca tcagtgccgg cggcgtgggc atcatcgcgc gccaggcgcg
     1681 ggtgcacatc gtcaacagca ccatcggcgg cacgcgcgga tcctatgaag cctccgaagg
     1741 cgccgaaatc tacgtggcgc gctccacttt caccggcatc ggtcgccgct tcgacacggc
     1801 caccatgaac gatctcggcg gcaacgcgta cgagccaaac taagctcacg cgaccgtacc
     1861 cggtgcgcgc gttcgccatc gttgttctgg cgtccgcttc gctggcggcc gccgctgatc
     1921 ccggaaactc ggcggcgctc gagcaatacc gcaagttgcg ctgcgagcct cgcagccttt
     1981 ccgtgcagtc cgtcgagcct ttgcgcttcg cggccaccgc aacctgctac aagctgccga
     2041 agtcgttcgc gcatgacggg tgggtacagc tcgtggcggt cgatgtcggc gccggcaccg
     2101 tgcgcctcgt cgacctcggt cgcgacaatg atgccgcaag gactttgcaa cacgacggcc
     2161 acggtgggtt gcgttggatc gcgcgccggc gaccgcacgc cgatcaatcc cgtgagcttg
     2221 aagtctggac gctggcggca tcggaaacgc aaccccggct gacaggccag ctcgaactac
     2281 ccttcgacgt cggcaacggg ctggacgcca cacggggcac gcaatgcatg ctcatcggcg
     2341 gaagtcccca gaatcatgtg ctggtgaaat tcatcgacgg cggactcagc gttcagcctg
     2401 tcgccggact gcgtggcgtg cggttgtggc attccgcgca tcaagccttc gtggtgcaga
     2461 cggatccaga atctctcgcc gttcacgcgt tactcgattg cgacgggcag acccggcccc
     2521 tgcccgaggg ggatgccgcg cgcttcgcgg agtttcctcg cgagcagatc ctcatttcga
     2581 gccgaagcga ctggctggtc cgcatcgagt ccagcgaggg cgaaggcact gagctcgtga
     2641 tcttccaggg cgacagccgc cgctcgttcg gccccttcga atggatgcca aaaggctgtc
     2701 cggatatctg cgtcgcggaa ggacttcagc tgatggaccc cggctggtct cccggcggag
     2761 gcgtgttcat tgcgcatgag ttcttcgagg ccatgatcgt gtccggcgag aaactgacgc
     2821 gcatcgccag ctggaattac cgtgataacg gcgccgccaa ccacacggcg ttgctgtcgg
     2881 actcacttgc catgcagcta tcgaaaaagc gcggagtcat cttccaccgt tggtaggatg
     2941 cggcctgatc acccacgagg aatttcgatg acttactgcg tcggcgtcca ggtcgatcaa
     3001 ggcctggtat ttctgtctga ctcaaggacc aatgccggcg tcgaccaggt cagtacattt
     3061 cgcaagatga ctttcttcga gatgccgggc gagcgggtca tcgtgttgct ggccgccggc
     3121 aacctggccg tgagccagtc ggtaaccggc ctgctgcgtg atcgggtgac ccaggccagg
     3181 aacagcctgc tgacgacgcg caccatggcc gacgccgcgc ggctcgtggg cgacgccgtt
     3241 cgcgacgtgt accaccgtga tgccgaaccg ctgagagagc acggcatcga gttcaacccc
     3301 actttcatca tcggcggaca gattggcggc gaggcgccgc gcctgttcca cgtgtattcc
     3361 gccggcaact tcatcgagac caccgcggat acgcgttatt tccagatcgg cgaatccaag
     3421 tacggcaagc cgatcatcga tcgagtggta aaggccgata ccgggctcgc cgcggtggcc
     3481 aaatgcgcac tcatttcgat ggattccacc atccgctcga atctgtcggt gggcatgccg
     3541 ctcgacctcg ccatcgtgcg ccgcgatgcg ctcgcgattt cgttgcatcg aagcattggc
     3601 ccggacgacg cctatttcaa agtcatccgt gacggctggg gtgagtcgtt acgtaatgcg
     3661 ttccacgccc tccccgaccc ctcgattccg cagtcctagt ggcgaaagtg ggacaacacc
     3721 gtgcggcgcg cacgttttgg cgcagccgtg tttccttccc gctgaatcag gcgtagcttc
     3781 ttttctcatg agagacccgc cgacaagccg tcgaacggca ctgcaggcgc tgctggctct
     3841 gggcggcagc ctgaccgtgc gggacctctt cgcagaaccg aacatcggca cccgacttcc
     3901 cggaacgccc gaagatcttt cgggtgcatt ggccgatccc gaacccctgt ttgccgcacc
     3961 cacgcgcctg gatcgcatcg gtcgcgtgat gacgcacgtg aaggtgaacg gcaagggccc
     4021 ttttcgtttc gtgattgaca ccggtgccag ccgctcgacg ctcgcgccgc atctcgcgcg
     4081 cgcgctcgcg ctgaaacctt cggtcggtcg caacgtcatg ctcaatggcg tcaccggagc
     4141 cgccgaagta cccacggtcg ccgtcgacag catcgaaatc ggcgcgctgc gttttgaaaa
     4201 gcaggatctt ccggtcatct tcacatcgat catgggcaac gccgacggca ttctcggcgt
     4261 ggccggtttt caggatcagc gcatcgacgt cgacttcaaa cgcgaccgcg tgagcgtgct
     4321 cgaatccaac ggcaagcggc cccactacag catggtcacc gcgcgggcca cgcgaaacgt
     4381 caacggactc atgatcgtcg acgtgcgtgt cgggcgccgc atccgcgcga aggcggtcat
     4441 cgacaccggc gccgaacgtt cgctgggcaa tctcgcgctt cagggcgcca tgaacaagaa
     4501 ccgcaagaaa cgccaggtcg tctcggcggt ggtgcacggc gcgacccccg acatcgccga
     4561 cggcgacgtg cagaccatca gggaagcaac catcggcgac atgacgctga ccaacctcga
     4621 agtgatcttc gccgacttcc atgtcttcaa gctctggggg ctcgaccagg aaccggcgct
     4681 gctcatcggc atggacatgc tgggcgtcct cgaacggctg gtcatcgact atcggcgcaa
     4741 tgaagtaagc atgtacggcg agcgcgcgac ctcgcgaatc gttcagcgct agcttcgttc
     4801 gcctcagcgc agcaacgtga aggcgccgag cgccacgcgc cggccgttga gcagcgcttc
     4861 caccttgtgt acacccgggt agtgtttgcg ggtagtcagt tgccgcagcc ctatcttctt
     4921 cgccacggcc acgcgcgcac ccggcgccag attgagcgcc ttcatcttga aggtcttggc
     4981 gcccgtgcca cgcgccttca cgaagtgcac caccaggtcg accatgactc gctgcggctt
     5041 ttgttgcttg ttgaccagcg tgaatgacag cgtgacggcg ccgccgattt tcactcgcgg
     5101 cggcgtcacg cgcttgtcgc gcagcgccac ctccgccttc gatccataac ccagcgcgcc
     5161 caacgcgccc aggtccgcac gtttcaccgc cgaacgcagc gcgtgattca cgatccaccg
     5221 ccgctcgacg gttgcgccgc gcagccatcg tttcgccacg gtgacgacca gcgcaggatg
     5281 atccttgccg atgtcattga ggttgttggc caccgagcgc cgaacataaa gctccgaatc
     5341 gtctttcagg agttcgagca gctcgagcac cggccgcgga tctttctgga acgcgcgcag
     5401 gcgcggcgcc cagggcaggc gcggccgcgt cccttcggaa accagtcgac gcacgtgtgg
     5461 gctcgcgtca cgagtccatt cgcgcaatcg ggccagcgtg gcgtcgggat gtttttcgag
     5521 aaacgcgcga atgctgtatt cggcggtgaa gcgttgcgtc aacgcgtgtt gcgcgcgcat
     5581 cgaatcttca aaatgatcga gtccgtgtcg cgccacgaag aacaggtgcg gcatgtacag
     5641 aaaagccgcc atgccgcccg aggcgcgatg cgcgtgcggt tgtgaggccg acgccagcag
     5701 cacgtcgatg gcttgcggat agtcggccgg caagtgtttg cgcagcgccg cggcgatgtg
     5761 aaatccgcgg ccggtcaggg tcatcgcggc aaatccggcc agtgcgtcgc gcaggaactc
     5821 gtcgtgcgga aagtccgcgt gcacggcgcg aatcatctgc gcgatggcgc gcggcgcgct
     5881 cggtccgaac tggtctttga gagcgctcgc caagcgacta ctgccgcgcg gtggccagct
     5941 tcaatcccag cgcgatgaac atggcgccgg cgaccttgtt gagtacgttc gccatggacg
     6001 gccggcgcag aaaagcgacg cgcagtttcg ccgcggcgat ggccagcacc aggcaccaga
     6061 ttacgccgag cgtcacgaac gtgagcccca aaaccaggaa cgcgccaacc ttccgcgaac
     6121 tgccggcatc gatgaactgc ggcatgagcg cgagaaagaa cagcgccacc ttgggattca
     6181 gcaggttgga aaagatgccc tggcgaaacg ccggccagcg gccggaatcg ctgcggactt
     6241 catcgccggg cagaccgctc gcgcggctca gcaatgcacg cacgccgata taaatgagat
     6301 aggcggcgcc cgcgaacttc accgccatga atgcataggg tgacgtggcg agcagcgccg
     6361 aaagcccgag cgcggcagcg aaggtgtgga tgacgctacc ggcggtgatc ccgagcgccg
     6421 cggaaattcc ttccgatcgc ccacccgcga gactgcgccc gagaatgaag aacgtatcct
     6481 gacccggcgt gatgaccagc aggatggccg tggcgatgaa cagccagtag ttatgaatgc
     6541 caagcatggg cggcactgtg ccgaactcgc ccctgcctgg caaacaagtt ttgcttacga
     6601 aacgaaagct gcgggcacgg gttcctcgcc cagcgcctgc cgcagcaccg cccagtgcat
     6661 cgcctcgtct ccgagaatgc tcgccgccgc cttggccaac tcgcgatcct tgaatagcgg
     6721 caccgcgcca agatacgcac tgaccgcgcc cttctcgagg cccgccgcaa agcgcagcac
     6781 atcgttctgc gtcttcagtg tttcgacggg aaagttgtac tgcgctttcg cgaccgccgc
     6841 cgttccaccg agcttggtca cggtgcccgc gagcacatcc gcatgttgct tgtggtggcc
     6901 ctggaacgtg gtggcgagat tcagcacggc gcccttgagc agcccgctgc cggctcctac
     6961 accgtatgcg gcgattgcct ctagttcggc cgcaagcgcg gtgttcagga ttgccacatc
     7021 agaagccggg ttcgcaccct tctccgccgc ggcggccagt gcatcgcggc cggcgagcag
     7081 tgccacggcg gcgcccgaaa gccccatgcc cccgacgccg aggaacgtac ggcgggcgac
     7141 gagaacattg ccgaatagcc gagtgtcaag gaaagacatg ggaatttctc cgtaggaaga
     7201 gtgcacctgt taaatgcctc gccgagcgga tttgttccca ggggaaccgg atttgagggc
     7261 cggggcactc acccctgaag gtcagctcat cgagcgggaa agggactcaa tgctctcaat
     7321 acttgtttgc cactcgtatt tcctgcgttt cgaccagaag caggtgaagc gcgccaagcc
     7381 ctatccgccg cttgcgacac tgcaggtggc agccatgttg cgcaatgccg gccacgaagt
     7441 gtcgttcttc gactccatgc ttgcggacga cctcacccgg ttcgaacgcc agctcgatgt
     7501 cttgcgcccg cagctcgtgg tgttctacga ggacaacttc aatttcctga gcaagatgtg
     7561 cctgggccgc atgcgtgaag cctgcgccga aatgatcggc ctggcccgcg cgcgcggtgt
     7621 acgcctcatc gccggcgggt cggatgcatc agacgcgcct ggaagttatc tcgccgccgg
     7681 cgcagacgcg gtgttgcacg gcgaaggtct cggcgctctt gtcgcgctcg tggaccggat
     7741 caatggcgac attcatctgc ccgcacatgc gctcgcggcg ggactggccg atgtgagcgt
     7801 cgcgggaatc tccgggcccg aacgtggccg cgccgcaggg ccactgcccg aacccgcgca
     7861 ggcgtccctg cccgcgtggg atctggtcga cgtcgagaaa taccggcgcg tgtggaaaaa
     7921 ggcgcacggc catttcagcc tcaacatggc gtcttcacgg ggctgttcgt tccgttgcaa
     7981 ctggtgcgcc aagcccatct ggggaaatca gtatgtgcag cgctcgccgc gcgaaacggc
     8041 ggcggaaatg atctacctgc gccggcacta ccgtcccgat cacatctggt tcgccgacga
     8101 catcttcgga ttcaaggccg cctgggtgaa tgaattcgcg gccgccctgg ccgaaggcgg
     8161 aggcggcgtg ccattcacca tccagttgcg cgccgacctg gtcagcgcac gcatgaccga
     8221 ggcgttgcgc gcggccggct gccgggaagt gtggattggc gcggagagcg gcagccagaa
     8281 gattctcgac gccatgaaca agggcacgac cgtcggtgaa accctccagg cgcgcaagtt
     8341 gttaggcgcc gcgggtatcc gcgtcggcct cttcctgcag ctcgggtacc tcggcgaagg
     8401 gctcgacgac atcctggcca cgcggaagct cgtggacgag gcgcgcccgg atgacatcgg
     8461 cgtcagtgtc tcctacccgc tgccgggcac gaagttctac gagcaggtca aagaacagct
     8521 cggcgacaag cgtcactggc gcgaaagcga cgatctggaa atgatgttcg ccggaactta
     8581 cacgtcggac ttttatcggc agttgcgcaa tctcctgcac gaccaggtga gtcacgcggg
     8641 cgatcgaacg ctgcagcagc gctggagcga tctcatcgag cgtgaatacg aataccgccg
     8701 caaccggcaa ccggagtttg gcggcggact gagtgtcgcg catgcctgac atcctgctca
     8761 ctcacggata tttcctcgcc gaagacgaga aagagcggca gatcatgaag ccgtatccgc
     8821 cgctcggcct gctctatcta tccgcattcc tcaagggcgc ggggttctcg gtcgaagtat
     8881 tcgacagcac gttcgaagag ttcagcgcgc tcgaagcgag aatggccgcc acgcccggcg
     8941 gcgtggtcgg catctatacc aacctgatga cgcgacgcaa cgtgctgcgc atcatccagt
     9001 ccgccagaca gcatcgctgg accgtggtgc tcggcggccc cgaaagcgcc aactacatcc
     9061 ccgagtatct cgacgccggc gccgacgtca tcgtctccgg cgaaggcgaa attacgctga
     9121 cggagttatt gcgcacgctg ccccagcagg gtgtgcaccg cctgcagggc gtacacggca
     9181 tcgcgtttcg cgacgagagc ggcacgctgg tgagcacccc cccgcgcctg cgcgtgcctg
     9241 acatcgactc cctgccgttt cccgaccgcg aggcgatcga ccacggccgc tacctcaacg
     9301 cgtggaaaac ccatcatggc gccagcagca tcaatctcat caccgcgcgc ggttgtgcgt
     9361 acaagtgcaa ctggtgctcg catgccgttt acgggcattc ccaccggcgg cgcagcccgg
     9421 ccaacgtggc cgatgaaatg cagtggatca tcgatcgcta cgaccccgac caggtctggt
     9481 acgccgacga cgtcttcacc atcagccacc cctggctcgc gagctacacc gccgagctca
     9541 agcgacgcgg aattcaccgg ccgttcgaaa cgatcacgcg cgccgatcgc ctgcaaaacc
     9601 agggcgcggc ggcgctgctg cgcgagctcg gctgctaccg gatctggatc gggtcggaaa
     9661 gtggcagcca gaaaatcctc gatgccatgc aacgcggcgt aaaagtggac caggtgcgtc
     9721 tcgcgtgccg cctcgcgcac gagcagggca tccaggtcgg catgttcctc atgtggggtt
     9781 acgagggcga agagatcgag gacatcgccg ccaccgtcga acacgtgaag caaagcaatc
     9841 ccgatgtgtt cttcacgacg gtgtcgtatc cgatcaaggg cacgggctac ttcgacaagg
     9901 ttcgcgatcg cgtggagatg ccgatcccct gggccgaagc gtccgaccgc gactacgtga
     9961 tcgccggccg tcgcggcaag gactactacc agctcgccga tgtctggcta cgcaacgaag
    10021 tggaagcgtt ccgtctcgag agcgccgatc ccgcgcgcgc cgcggagtta tggggccgcg
    10081 cgcaacaggc gcgcgagtcg ctgcaaagct ggaaggttgc atgagcaacc tgcatcccga
    10141 cctgccgccg ttcgccgtcg tcgaggccgc gttacacaag accacggcgt tcctcgcgcg
    10201 cgagttcagc gcaccctcgg aagccgctcc cgattggaac gaattcgaat gggacgtggc
    10261 cagttcggtc agcgcgatgc agggaatttc cgcacttctt gcggggcgcc tgaagtgggt
    10321 tggcccgccc gcgtggcaat ccttcctggc agatcaggcc caccacaccc gtgtgcggga
    10381 cgagcggttc ggcaaactcc tcgcgacgct ggaacgcgaa atgaatgctg ccggcatccc
    10441 catcttgccg ctcaagggct cggcgctgcg cgatctgcag gtccacgctc cgggagttcg
    10501 cccgatgagc gacatcgacc tgctggtcag cccgcgggac gtcggcgaca gcatcatggt
    10561 gttgcggaga atcggctacg tggccaagtt ctcgacgcat cgccatctgg tgctcgcacc
    10621 gccggacatg cggatcccgc agtgccttgg cgagcattgc gacgcgccat acaagatcga
    10681 gctgcacacg gcgatcgccg aagcactgcc tttcacgaag gtggacatca ccgaccgcat
    10741 ccgcgcgcag actcagcggg ccggcatcaa ccgctacccc tccgcgcagg cactgatgtg
    10801 tcacctgctg ctgcatgccg cgggcaacat gcgcgcgaac gcgttgcgcc tgatgcaatt
    10861 gatcgacatc gccatgctcg cgcggcgatt tggcccagcc gactggctgg ctctcctcga
    10921 cacatcgcca cccgcgcatg cctggtggat gtatccgccc ctggcgatga ccgcgcgtta
    10981 tctccccggc gaaattccgc cggccgcatt gaacatggct tacgacatct gcccacgcca
    11041 gctgcgccgg aaggcggcgc gacacacgct ggagaccgtc tcctggtcga atctccgcat
    11101 cgcggcgctg ccgggttacg agtggtcacg caatgcgctc gagctgatgc gcttcgcgcg
    11161 cagccgcatc ctgccgcggc gtgagtcaac caatgaactc gccgacgcag tcaatcatca
    11221 gcctgacctg ctgcgcattc cgtggtacca gcagtcgcag gcgaatcgga tcgtgcgatg
    11281 gctggtgtcc cgcccccctc gcgtgcagac gataatgacg ctgctcgccg gccagacgcc
    11341 gcgatgacgc tgcgcgacga cgaaccgccc gtttcctgcg agccgtggcg ctcgtcctcc
    11401 gcgccccgcc gcatccttgc catgcgcttc caggcgctgg gcgacaccat catcacgctc
    11461 ccctatctac tgagcctgaa gcggcgcttc ccggaggcga ggctgcattt cctcacacgc
    11521 aaggaagtct gccaggtgcc cgcggcggtg gacctgttcg accgcgtcat cgcgctcggc
    11581 ggaggccgca atctcaagct gcagttgttg agcgccgcct ggtggatgcc ctggctgctc
    11641 gcgcagcgtt acgacctcgt gctcgacctg cagaaccacc gcatcagccg ctggatccgc
    11701 aagttgatcg cgccgcgcgc gtgggccgag ttcgactgct attcgccgcg ctcggccggc
    11761 gagcg
//
LOCUS       DASQJS010000127        10258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4148949, whole genome shotgun sequence.
ACCESSION   DASQJS010000127 DASQJS010000000
VERSION     DASQJS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10258
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4148949"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..719)
                     /locus_tag="VEW08_05270"
     CDS             complement(144..719)
                     /locus_tag="VEW08_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYJ40179.1"
                     /translation="MVAGLHVAVLFVIARSLGIGAPVEVPPPLVTEIYKETRPPEELP
                     RIPEPDVTTTRESLVLPDPDIQTMAFETDTITGPPPDAVFDDDPPPATPQPKLVGVRQ
                     DPRHPLSKPEYSPRMIREGNQGTIEVEVYVMPNGRVADARVVKSTGFAELDLAAIAEA
                     KRNWRLLPATRDGEPFAQWHRLRVTFKLNER"
     gene            1001..2326
                     /gene="rimO"
                     /locus_tag="VEW08_05275"
     CDS             1001..2326
                     /gene="rimO"
                     /locus_tag="VEW08_05275"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HYJ40180.1"
                     /translation="MSRRQPKVGFVSLGCPKALVDSERIITQLRAEGYQVAASYDAAD
                     VVVVNTCGFIDSAVDESLDAIGEALEQNGKVIVTGCLGARPEKILDRHPRVLKVTGPQ
                     AYEDVMNAVHEHLPPKHDPFIDLVPPQGIRLTPRHYAYLKISEGCNNKCSFCIIPSMR
                     GLLASRPIDDVLREGEKLARAGVRELLVISQDTSAYGVDIRYREAEWRGQKYETRFLD
                     LARGLGELGIWVRMHYVYPYPHVDDVIPLMAERKVLPYLDIPFQHGSPTVLKRMRRPG
                     QVEKTLSRIKAWRGVCPDLTIRSTFIVGFPGETEAEFEELLAWLDEAQLDRVGAFKYS
                     PVEGAAANDVAPHVAPEIQEERLARFMQKSAGISAAKLAAKVGKSMRVLVDSIEGTKA
                     IARSEGDAPEIDGVVRIVKGGKLKIGEFADVEITGSSDHDLEAKVLRPN"
     gene            complement(2513..3670)
                     /locus_tag="VEW08_05280"
     CDS             complement(2513..3670)
                     /locus_tag="VEW08_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYJ40181.1"
                     /translation="MYMKSCTLLLALALGLTGPGDGHKPFTVTSGLFDQSKPDLGLHA
                     APKSETISIFKPLADTDHFSNGVVLLPFKGRLYAQWQSSPRDEDSQDTWVAYSVSDDG
                     SRWSAPAVLAPAGKSPQMHSSGGWWTDGKTLVAFINVWPTGFQSGEGGYTDYRLSTDG
                     QHWSAPRRVTGKDGKPVPGIIEQDPHAMSGGQLVTAFHMRPGTIVTPFYTDDALGISG
                     WRRGVMQNLPHEGNSSRELEPSTFMRGTCVVMVFRDQAESFQQLASESCDSGQTWTGP
                     AFTGMPDSRAKQSAGNLPDGTAFLVNAPRADRVRIPLAITLSRDGRIFDRAWTLRGTA
                     DLQPMRYEGLYKRPGYHYPKSVIWKEHLYVGYATNKEDVELTRVPLVSLRR"
     gene            complement(3681..4553)
                     /locus_tag="VEW08_05285"
     CDS             complement(3681..4553)
                     /locus_tag="VEW08_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HYJ40182.1"
                     /translation="MTSATYLPFILLLTAVLGLWVHRAVWIAALAAAVVAGIFTGALH
                     GLAAVWIALLVALALAYTGVRDHSTSPNKPILQTLAGLAFFVYALAMGLALLPGFHRV
                     VLAQPVVLSAGAAPHGISVGFPKVVTGIIILGLINPLLVHSWRELGRVLARALPVFAI
                     TATVGMAVVLLMGYTSLAPKWTALFLLFAPVNLFFTCLSEEAFFRGFVQHELSRVGSR
                     PALAAGIALTVAAILFGLAHFGGGTSYVIAAAVAGLGYGWAFLRTQRIEAAMAVHFGV
                     NAVHFLLFTYPRLA"
     gene            complement(4636..6270)
                     /locus_tag="VEW08_05290"
     CDS             complement(4636..6270)
                     /locus_tag="VEW08_05290"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HYJ40183.1"
                     /translation="MKKTTRRDALKSIVVGGAAVATSSAQAAKPKDPQDACAATLRTD
                     WSDLRWGKGFEGQRIADLGDGTFLNPVFSGDRPDPTILKDGADYWMTFSSFDSYPGIV
                     VWHSRDLVNWNAVGPALTKPVGSVWACDIAKHGGRYFIYFPARTAARRSNYVVHAESM
                     RGPWSEPIDLNLPAHIDPGHIVGEDGKRYLFLSGGDRVKLKDDGLATDGAVEHVYDPW
                     RYPDDWVVESFSPEGPKVLRHGQYFYMVTAVGGTAGPPTGHMVIAARSMSINGPWENC
                     PANPLVRTTDVREKWWSRGHASLVEGPGGDWWSVYHGYENAYWTLGRQTLLDRIEWTD
                     DGWFRFLGKDLSQPIPKPAKLRNQTHGIALSDDFSKDKFGIQWAFYSPGENERARVAY
                     EAGAMVIAGKGDSPSNCSPITCLTGDQAYRCEVEIEVEGAATGGVLFFYNKRMYCGLG
                     FNERQLVRHRTAIDAPRAKPKGIDRRLFIRFENDKHIVTFHTSPDGKTWTKYDVQMEV
                     SGYHHNVAYDFLSLRPGLYASGDGKVRFRNFKYQAI"
     gene            complement(6362..8908)
                     /locus_tag="VEW08_05295"
     CDS             complement(6362..8908)
                     /locus_tag="VEW08_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HYJ40184.1"
                     /translation="MIRNFRVAALAMPMLLVGCLYPYFPTADAGADQSATEGAVVHLK
                     GSGTDVDGKVVDYQWRQVSGPTVKLSNANKKNAKFTAPDVEIQTSLEFELVVKDNYGA
                     PSEPDTVTVLVDQIKFFGTAPGGPEDYEHLLTYFDQITPENAGKWGSVEGTRDTMNWT
                     ALDTAYQTAVANNLKFKFHTLLWGQQQPGWLTGLPAAEQLEEIEEWMAAVAARYPDLE
                     YVEVLNEPLNAPPPYREALGGAGTTGFDWVIKSFELARKHFPKARLILNEYNVIVENG
                     ITTNYLNIINLLNERKLIDGVGEQAHFYERANPEVLLGNLDRLTATGLPVYISEFDLN
                     LANDADQANVMSKLFPIFWDHPGVAGVTHWGHQQGSMWRANAFLVRTDGTTRPAMDWV
                     QCYIGGGGDACTVPEFVHPGWVGDQNGVTLDAVNHDEGSGVVSGGVIAYTDDGDWIKF
                     KGVQFRSDWNKVWVAYAKGNTTQGSISVHLDSLDSPAAVTVNLPPTAGWGSSATVEEA
                     LASISGTHDVFIRFNGGPGVANLSSVRFGKPIPPSTTNLLNNGGFEAGIAGWSNWGSG
                     SLSVSTAQAHGGTQSLLSTGRTGTGGFAAYNLTSLVQRGTTYAVGAWLLHTGAANTTG
                     RLAAKVECTVATTPAGHNTYPWLQNNGAVAPNTWTQLAGNLVIPNCDLVEVSIYFEGT
                     AAGVDVYLDDIRVVPPNNNLVNDGGFETGIAGWSSWNGSMLSASGLQKRNGAQSLRAT
                     ARPDANQFAVYNLTSRVTAGNTYAVSAWVFQTGAANDVVRLASKVGCSAGDTYPWLQN
                     NAAVVPNTWTQLSGNLVIPAGCTVVDVVIFLEGTPIGADVFVDDVSVTAP"
     gene            complement(9147..9404)
                     /locus_tag="VEW08_05300"
     CDS             complement(9147..9404)
                     /locus_tag="VEW08_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40185.1"
                     /translation="MERKIAEQIVALVNGTIDQLIATLEPVEAAVSPDEFAAYKRGIA
                     RVIAAFDTGVIDLVAREHPDLKPNDDDTEVPEAADPRVSRN"
     gene            complement(9479..>10258)
                     /locus_tag="VEW08_05305"
     CDS             complement(9479..>10258)
                     /locus_tag="VEW08_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HYJ40186.1"
                     /translation="ETADTLAALRLAKQAGYLSSLAICNVPESSLVRESELVMLTRAG
                     PEIGVASTKAFTTQLTALSMLVIALAKHRTADAERERSLVQRLIEIPSVIEKTLALDP
                     IIYDLAKRFADKQHALFLGRGALHPIAMEGALKLKEISYIHAEAYPAGELKHGPLALV
                     DADMPVITVAPNNDLLEKLKSNLMEVRARGGELIVFADPDSGFVSSDGVTVILMPKHV
                     SYFQAPAVYTVPLQLLAYHVAILKGTDVDQPRNLAKSVTVE"
BASE COUNT         1856 a         3164 c         3371 g         1867 t
ORIGIN      
        1 agtggccatg gatcctggtg cccattgccg cgttcagcat tttcttcggc ctgcgcgaat
       61 gccagcaccg gatcccgccc gccgaacacg cggcgccggt cgaaccggcg ccgccctctc
      121 cgacggaaca atagccccgc cgattaccgt tcgttcagct tgaaagtgac gcgcaggcgg
      181 tgccactgcg cgaacggctc gccatcgcgc gtggccggca gcaggcgcca gttgcgcttc
      241 gcttcggcaa tggccgcaag atccagttct gcaaagcccg tgctcttgac cacgcgggcg
      301 tcggccacgc ggccgttcgg catcacgtag acctcgactt cgatggtgcc ctggttgcct
      361 tcgcggatca tgcgaggcga gtactcgggc ttcgacaacg ggtggcgcgg gtcctggcgg
      421 acaccgacga gcttcggttg cggtgtcgcg gggggcgggt cgtcatcgaa gacggcgtcc
      481 ggcggtgggc ccgtaatcgt gtctgtctcg aacgccatcg tctgaatgtc ggggtccggc
      541 agcacgaggc tttcccgagt cgtggtcacg tccggttcgg gaatgcgtgg cagctcttct
      601 ggcggccgcg tttccttgta gatctcggtg accaggggtg ggggaacttc aacaggcgcc
      661 ccgatcccca ggctcctcgc gatgacaaac aacacggcga cgtgaagtcc cgcgaccaag
      721 cccattcgac ctaatgcgcc aagtgggcgc ctctgcgcag caacagcagc catgacggac
      781 ctccgcatgc acccggtgtt aggggtgtct tgagaggtga acgcgcgcaa aaatggatat
      841 gggttagagc tgaccgctgc acccacaatc tatcggattc aattgtcggg agtaagaata
      901 tggatcaaaa cagcgtggcg ggcctgtttt tacccgcaat tgccatatgt ccacgccgcg
      961 tcgcccggtg ggccgcgcgc gtgtaccatt gcgccccctc atgagccgcc gccagccaaa
     1021 agtaggattc gtcagcctcg ggtgccccaa agcgctcgtg gactctgaac gcatcatcac
     1081 gcagctgcgc gccgagggct accaggttgc ggcgagctac gacgcggccg acgtggtggt
     1141 cgtgaacacc tgtggtttca tcgactcggc tgttgatgaa tcgctcgacg cgattggcga
     1201 ggcgctggag cagaacggca aggtgatcgt caccggctgc ctcggtgccc ggccggaaaa
     1261 gattctcgat cggcatccgc gggtactgaa ggtcacgggg ccgcaggcct atgaagatgt
     1321 gatgaacgcg gtgcacgagc atctgccgcc gaagcacgat ccgttcatcg acctggtgcc
     1381 gccgcagggc attcgcctga cgccgcgcca ctacgcgtat ctcaagatct ccgagggctg
     1441 caacaacaag tgcagcttct gcatcatccc gtcgatgcgc gggttgttag ccagtcggcc
     1501 catcgacgat gtgctgcgcg aaggcgagaa gctggcgcgc gcaggagttc gcgagctgct
     1561 ggtgatttcg caggacacct cggcctatgg cgtggacatc cgctaccgcg aagcggagtg
     1621 gcgcgggcag aaatacgaaa cgcgtttcct ggatttggcg cgcggtctcg gcgagcttgg
     1681 catctgggtg cgcatgcact acgtgtatcc gtatccgcac gtggatgacg tgatcccgtt
     1741 gatggcggag cgcaaggttc tgccctatct ggatatcccg ttccagcacg gcagccccac
     1801 ggtgctcaag cgcatgcgcc gcccgggaca ggtggagaag acgctgtcgc gcatcaaggc
     1861 ttggcgcggg gtgtgcccgg acctgacgat tcgctcgact ttcatcgttg gcttcccggg
     1921 cgagaccgaa gccgagttcg aagagttgtt agcttggctg gacgaagcgc agctcgatcg
     1981 ggtgggcgcg ttcaagtatt cgccggtgga aggcgcagcc gcgaacgacg tggcgccgca
     2041 cgtggcgccc gaaattcagg aagagcgcct cgcgcgcttc atgcagaagt ccgcgggcat
     2101 cagcgcggcc aagctcgccg cgaaagtggg caagtcgatg cgcgtgctcg tggattcgat
     2161 cgaaggcacg aaagcgatcg cgcgaagcga gggcgatgcg ccggagatcg acggcgtggt
     2221 tcgcatcgtc aagggcggca agctcaagat cggcgagttt gcggacgtcg agatcaccgg
     2281 ctcgagcgac cacgatctcg aggcgaaggt actccgaccg aactagactg cgccgatgat
     2341 gcgatccatc gacgcagagc actacgttct cgcgctcaag ctgaagcgcg acaaaatccc
     2401 ttcgttcgat ttctatccct ggtcactcgc ggcgatccgg cgcctcggga ctctcgagtt
     2461 tcatcccgcg gttaccttca tcgttggcga gaacggcagc ggcaagtcga cctcagcgac
     2521 gcagactgac cagcggcacg cgcgtgagct ccacatcttc cttgttcgtg gcgtaaccca
     2581 cgtacaggtg ctctttccag atcacgctct tggggtagtg atatcccggt cgcttgtaca
     2641 acccctcgta ccgcatcggt tgcagatcgg cggttccacg caaagtccac gcgcgatcga
     2701 agatccggcc atcgcgactc agcgtgatag cgagcgggat gcgcacccgg tcggctcgcg
     2761 gcgcattgac aagaaacgct gtgccgtcgg gaagattgcc ggcactttgc ttggcgcgcg
     2821 aatccggcat gccggtgaaa gcgggcccag tccaggtttg gcccgaatcg cagctttcgg
     2881 aagccagctg ctgaaagctc tctgcctggt cgcggaacac catcaccacg cacgtgccac
     2941 gcatgaaagt gctgggctcg agctcgcggc tcgagtttcc ctcatgcgga aggttctgca
     3001 tcacgccgcg ccgccagccg ctgatgccca acgcatcgtc cgtgtaaaag ggagtgacga
     3061 tggtgccggg gcgcatgtga aacgcggtga cgagctgccc gccggacatg gcgtgtggat
     3121 cctgttcgat gatgccggga accggcttgc cgtctttgcc ggtcacgcga cggggcgctg
     3181 accagtgctg accatcggtg ctgaggcgat agtcggtata gccgccctca cccgactgaa
     3241 atcccgtggg ccacacgttg atgaaggcga cgagcgtctt gccgtcagtc caccagccgc
     3301 cgctcgaatg catttgcggc gacttgcctg cgggagccag caccgcggga gctgaccagc
     3361 gcgatccgtc gtcactgacg ctgtacgcaa cccaggtgtc ctgcgagtcc tcatccctgg
     3421 gagaactctg ccattgcgcg taaagcctgc ccttgaatgg cagcagcacg acgccgttac
     3481 tgaaatggtc agtgtcggcg agcggcttga agatggagat cgtctcgctt tttggagcgg
     3541 cgtgcaggcc cagatcgggt ttgctctggt cgaacaagcc cgaggtgacc gtaaacggct
     3601 tgtggccgtc accgggacct gtcaatccca gggcgagcgc aagtagcaag gtgcaggact
     3661 tcatgtacat cgcgacttgg tcaggccagg cgcggatagg tgaacagcag gaagtgcacg
     3721 gcgttcacgc cgaagtgcac ggccatcgca gcctcgattc gctgcgtacg caggaacgcc
     3781 cagccgtagc caaggccggc caccgcggcg gcgatgacgt agctagtccc accgccgaag
     3841 tgcgcgaggc cgaacaggat ggcggcgacg gtcagcgcga tacccgcggc cagcgccggg
     3901 cgggaaccga cacgcgaaag ctcgtgctgc acgaagccgc ggaaaaaggc ttcctccgac
     3961 aggcaggtga agaacaggtt gaccggcgca aacaggagga acagtgcggt ccacttgggc
     4021 gcaagcgagg tgtagcccat gagcagcacc acggccatgc ccaccgtcgc ggtgatggca
     4081 aaaactggca acgcgcgcgc cagcacgcgg ccgagctcgc gccaggaatg cacgagcaat
     4141 ggattgatga gcccgagaat gatgatgccg gtgacgacct tggggaagcc gaccgagatg
     4201 ccgtgcggcg cagcgccggc agacagcacc accggctgcg cgagaaccac gcgatggaag
     4261 cccgggagca gcgccaggcc cattgccagc gcgtagacga agaatgcgag gccagccagc
     4321 gtttgcaaga tgggcttgtt aggcgaggtg gagtgatcgc ggacgccggt gtatgcgagg
     4381 gccagcgcga cgagaagtgc gatccagacg gccgcgagcc cgtgcagagc gccggtgaag
     4441 atgcccgcga ccacggcggc agcgagcgcg gcgatccaga cggcgcgatg cacccacaag
     4501 ccaaggacgg cggtgagcag caggatgaat ggcaggtagg tggctgacgt cacgatgaga
     4561 ttgaggtgcg gcccattttg ctaggtaaac gagtggggac agacttaagt ctgtccccac
     4621 acatccatgt ccccgttaaa tcgcctgata cttgaagttt cggaagcgaa ctttgccatc
     4681 acccgatgca tagagaccgg ggcgcaggct caggaagtcg tacgcgacgt tgtggtgata
     4741 gccggagact tccatctgca cgtcgtactt ggtccacgtc ttcccgtcgg gtgaagtgtg
     4801 gaaagtgacg atgtgcttgt cattctcgaa gcggatgaac agccgccggt cgatgccctt
     4861 cggtttggcg cgcggggcgt cgatggcagt gcgatggcgc acgagctgac gctcgttgaa
     4921 gccgagcccg cagtacatac gcttgttgta gaaaaagagt actccgccgg tggccgcgcc
     4981 ctccacttcg atctcgactt cgcagcggta ggcctgatcg ccggtgaggc acgtgatggg
     5041 cgagcagttg gacgggctgt cgcctttgcc tgcgatcacc atggcgccgg cctcgtacgc
     5101 cacgcgcgcg cgttcatttt cgcccggcga gtagaaggcc cactggatac cgaacttgtc
     5161 cttgctgaag tcgtccgaca gcgcaattcc atgagtctgg ttcctgagct tcgcgggctt
     5221 cggaatgggc tgcgaaagat ccttgccgag gaagcggaac cagccgtcgt cggtccattc
     5281 gatgcgatcg agcagcgtct ggcggccgag cgtccagtac gcgttttcgt aaccgtgata
     5341 gacgctccac cagtcgccgc ccgggccttc gaccagcgac gcatgaccgc gcgaccacca
     5401 tttctcgcgc acgtcggtgg tgcgcaccag cgggttggca gggcagtttt cccagggacc
     5461 gttgatcgac atcgaccgcg cggcgatcac catgtggccg gtgggtggcc ccgccgtgcc
     5521 gccgaccgcg gtcaccatgt agaagtattg gccgtgcctg agcaccttcg gaccttcggg
     5581 cgagaaactt tccacgaccc agtcgtcggg atatcgccac ggatcgtaaa catgttcgac
     5641 ggcaccatcg gtagccagtc catcgtcctt gagcttcacg cggtcgccgc ccgagaggaa
     5701 caggtagcgc ttgccgtcct cgccgacgat gtgccccggg tcgatgtgcg ccggcaggtt
     5761 gagatcgatg ggctcgctcc acggtccgcg catcgactcc gcgtgcacga cgtagttaga
     5821 gcgccgggcc gcggtgcgcg ccggaaagta gatgaagtaa cggccaccgt gcttcgcgat
     5881 gtcgcaggcc cacaccgaac cgactggctt cgtcagtgcc ggacccacgg cgttccagtt
     5941 cacgagatcg cgcgaatgcc agacgacgat gcccgggtag gagtcgaacg acgagaaggt
     6001 catccagtag tcggcgccat ccttgaggat ggtgggatcg ggacgatcgc ccgagaacac
     6061 cggattgagg aaggtgccgt cgccgagatc ggcgatgcgc tggccttcga agcccttgcc
     6121 ccagcgcagg tccgaccagt cagtgcgcag ggttgccgca caggcgtcct gtggatcttt
     6181 cggcttcgca gcctgggcgg agctcgttgc cacggcagcg ccgcctacga cgatggactt
     6241 cagcgcatcg cgacgagtgg tttttttcat ggcgcgaacg atagccagaa ttgtcaccgg
     6301 tgtcatgacg ggcaaaaaga aagggggctg caatgcagcc ccctctcatc gagacgggat
     6361 gtcagggtgc cgtcaccgaa acatcatcca caaagacgtc cgctccgatc ggcgtgccct
     6421 cgaggaagat gaccacatcc accacggtac agcccgcggg aattaccaga tttcccgata
     6481 gctgcgtcca ggtgttcgga accaccgcgg cgttgttctg cagccaggga taggtgtcgc
     6541 cggccgaaca gcccaccttc gacgcgaggc gcacgacgtc gtttgcggcg ccggtctgga
     6601 acacccaggc gctcacggcg taggtgttgc cagcagtcac gcgactggtg aggttgtaca
     6661 ccgcgaactg gttggcgtcc ggccgcgccg tggcgcgcag gctctgcgca ccattgcgtt
     6721 tctggagccc gctggcgctc agcatcgagc cgttccacga actccagccg gcgatgccgg
     6781 tctcgaaacc accgtcattc accagattgt tgttgggcgg aaccacgcga atgtcgtcca
     6841 ggtacacgtc gacgccggcg gccgtgcctt cgaagtagat ggacacttcc acgagatcgc
     6901 agttgggaat gacgaggttg cccgcgagct gcgtccaggt gttcggtgcc accgcgccat
     6961 tgttctgcag ccacggataa gtgttgtggc ccgcgggcgt cgtcgccacg gtgcactcca
     7021 ccttggccgc gaggcgtccg gtggtgttgg ccgcgccggt atgcagcagc cacgcgccga
     7081 cggcgtacgt ggtgccgcgc tggaccaggc tggtgaggtt gtacgccgcg aagccgcccg
     7141 tgcccgtgcg gcccgtcgac agcaggctct gcgtgccgcc gtgcgcctgc gcggtgctga
     7201 cgctcaaact accgctgccc cagttgctcc agccggcaat gcccgcctcg aagccaccgt
     7261 tgttcagcag gttggtggtg gacggcggga tcggcttgcc gaagcgcacg ctgctgaggt
     7321 tggcgacgcc cggtccgccg ttgaagcgga tgaagacatc gtgcgtaccg ctgatcgagg
     7381 cgagcgcttc ctccacagtg gcggagcttc cccacccggc ggtgggcggc aggttcaccg
     7441 tgacggccgc gggactatcc aggctgtcga ggtgaacgga aatgctgccc tgcgtggtgt
     7501 tgcctttggc ataggccacc cagaccttgt tccagtcgct gcggaactgt acgcccttga
     7561 atttgatcca gtcaccgtcg tcggtgtagg cgatgactcc gccggagacc acgccggagc
     7621 cttcatcgtg attcaccgcg tccagcgtga cgccgttctg atcgcccacc cagccgggat
     7681 gcacgaactc cggcaccgtg caggcatcgc caccgccgcc gatgtaacac tgcacccaat
     7741 ccatggccgg acgcgtcgtg ccgtcggtgc gcaccaggaa ggcgttcgcg cgccacatgc
     7801 tgccctgctg gtgaccccag tgcgtgacgc ccgcgactcc cgggtgatcc cagaagatcg
     7861 ggaacagctt gctcatcacg ttggcctggt ccgcgtcgtt ggccaggttc aggtcgaact
     7921 ccgagatgta gaccggcagt ccggtcgcgg tgagacggtc caggttaccc agcagaacct
     7981 cgggattggc gcgctcatag aagtgcgcct gctcgccgac gccgtcgatg agcttgcgct
     8041 cgttcagcag gttgatgatg ttgaggtagt tggtcgtgat gccgttttcg acgatcacgt
     8101 tgtactcgtt gaggatgagc ctggccttcg ggaagtgctt gcgcgcgagc tcgaaggact
     8161 tgatcaccca gtcgaagccg gtggtgccgg cgccgccgag cgcttcgcgg tagggaggcg
     8221 gtgcgttcag cggctcattg agcacttcga cgtattcgag gtccggataa cgcgcggcta
     8281 ccgccgccat ccattcctcg atttcctcga gctgttccgc ggccggaaga cccgtcaacc
     8341 agcccggctg ctgctggccc cagagcagag tatggaattt gaacttgaga ttgttcgcga
     8401 ccgcggtctg gtaggcggtg tcgagcgccg tccagttcat ggtgtcgcgc gtgccctcga
     8461 cgctgcccca tttgccggcg ttttccggcg tgatctggtc gaagtaggtc agcaggtgtt
     8521 cgtagtcctc gggaccgcca ggcgccgtgc cgaagaactt gatctggtcc accaacaccg
     8581 tgaccgtgtc gggctcgctg ggcgcgccgt agttgtcctt gaccacgagc tcgaattcga
     8641 gcgaggtctg gatctcgacg tccggcgccg tgaatttcgc gttctttttg ttggcattgc
     8701 tgagtttcac ggtgggcccg gaaacctggc gccactggta atcgacgacc ttgccgtcga
     8761 cgtccgtgcc gctgcctttg aggtggacca cggccccttc ggtcgcggac tgatccgcgc
     8821 cggcgtcggc agtgggaaag taaggataca aacagccgac aagcagcatc ggcatcgcca
     8881 gcgcggcgac gcgaaaattc cgaatcatgg ttttccccct ctgattgacc gactcgtctg
     8941 agcgagtgtg tgacgtaacg cggggtgctc cgcgtgcacc ttaccgcgcc acccttgtac
     9001 cagaagccgg gcgatacccg aagcccgtac aaatacgcca gcgcgacgca gcatcaatct
     9061 actcgccggg cgaggcgagc ttcgactaaa ggttgagaat ccccgcctgg acgttctgcc
     9121 cgatgtcctc ggccaggtcc cgggtgtcag ttccgcgaga cgcgagggtc ggcggcttca
     9181 ggcacttcgg tatcatcgtc gttgggcttg agatccggat gctcgcgcgc gacgagatcg
     9241 atgacgccgg tgtcgaaggc agcgatgacc ctggcgatgc cacgcttgta ggccgcgaac
     9301 tcatcgggcg agacggctgc ttcgacgggc tccagcgtgg cgatcagctg atcgatggtg
     9361 ccgttgacga gcgcgacgat ctgttccgct atcttccgtt ccattgaggc ctgacccttt
     9421 ttgctaggta aaagcgtggg gacagactta agtctgtccc cacacatcca tgtccccatt
     9481 attcgacggt tacggacttc gcgaggtttc gcggctggtc gacgtcggtg cccttgagga
     9541 tggcgacgtg atacgcgagc agctgcagcg gcacggtgta cacggcgggg gcctggaagt
     9601 aactcacgtg cttgggcatg agaattaccg tgacgccatc cgaggagacg aagcccgaat
     9661 ccggatcggc gaacacgatg agctcgccgc cgcgtgcgcg cacttccatg agattgctct
     9721 tgagcttctc gagaaggtca ttgttgggcg ccacggtgat gaccggcatg tcggcatcca
     9781 cgagcgccag cggcccgtgc ttgagctcgc cggcgggata cgcctcggcg tggatatagg
     9841 aaatttcctt gagcttgagc gcaccttcca tcgcgatggg gtgcaaggcg ccgcgcccga
     9901 ggaacagcgc gtgctgcttg tcggcgaagc gcttggccag gtcgtagatg atcggatcca
     9961 gcgccagcgt cttctcgatg accgaaggaa tctcgatgag cctctggacg agactgcgct
    10021 cgcgctccgc gtcggcggtg cgatgcttgg ccaacgcgat gaccagcatg ctgagcgccg
    10081 tcagctgcgt ggtgaacgcc ttggtggatg ccacgccgat ctcggggccg gcacgcgtga
    10141 gcatgaccag ctccgattcg cgcaccagcg agctctcggg aacgttgcag atggccagcg
    10201 acgacagata accagcctgt ttcgcgagcc gcagcgcagc cagcgtgtcg gccgtctc
//
LOCUS       DASQJS010000128        15774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1321961, whole genome shotgun sequence.
ACCESSION   DASQJS010000128 DASQJS010000000
VERSION     DASQJS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15774
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321961"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..291)
                     /locus_tag="VEW08_05310"
     tRNA            complement(216..291)
                     /locus_tag="VEW08_05310"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(256..258),aa:Thr,seq:cgt)
     gene            complement(406..840)
                     /locus_tag="VEW08_05315"
     CDS             complement(406..840)
                     /locus_tag="VEW08_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HYJ40187.1"
                     /translation="MPMSDLDPGLVENLNQRLLGHGVRPTAQRLRIASLLLGRPQHLS
                     ADQVLAGLRGLGLRVSKATIYNTLNLFAASGLIRQLSVGSDRCWFDSNTDAHYHFHDL
                     DSGALMDVSLSDVEFQRLPEPPPGMQVDGIDLVIRLRRRTEA"
     gene            946..1446
                     /locus_tag="VEW08_05320"
     CDS             946..1446
                     /locus_tag="VEW08_05320"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HYJ40188.1"
                     /translation="MCIKNRDRGVTLVELCFGLAIVAVIAGLAAPGFRSTLRAAAVRS
                     ATFDLLAGLQTTRGNAILESRPLDLCPSDSTGKCLAAGVPTPYWRSSLEAGAAHSLRA
                     SHVLPAGVVVRASRSPLWFSPDALGASTGTLTICDQQGVAPPRAIVLSQSGRARLEAA
                     HAEACQ"
     gene            1443..1925
                     /gene="pilV"
                     /locus_tag="VEW08_05325"
     CDS             1443..1925
                     /gene="pilV"
                     /locus_tag="VEW08_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HYJ40189.1"
                     /translation="MNRTTRGFTLLETLVSLVVLSVGLLGAAALLLDNLRTHAGALRR
                     VAALSLVRDMADRIRANPRGGVYYDTRMPAPAAAPCGGPGGCDVAQLAAADLAYFEST
                     AHALFPRADTNTRVEYAPATGHATPARHIITLRWSDARNDAGTDSVALQVLTLSPVAG
                     "
     gene            1928..2890
                     /locus_tag="VEW08_05330"
     CDS             1928..2890
                     /locus_tag="VEW08_05330"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HYJ40190.1"
                     /translation="MRRPSRAGGFNLPELMISLTLGLFLVAAFLLVLQRCRASFAANE
                     SLARLQDAARHAFSVLVPDIEHAGFYGFSHARAVRLVGSLPAGAVACGTNFALSLSVP
                     VQGSDNRFRLGADARDCAPTASAGGARAETDTLTLRHASLSPVAPRAGRLQIYSQNLS
                     IATPPLLFADGRAPVPVDPDHVVHDLEVRTYYIANDSVQRPGWPALRVKALTESRGAA
                     QFRDEEILPGVEDLQIEMGVASIAADGIPRVTYVGPDSPQARDGNVVAVRFWLRIRAD
                     ATEPGFSDGRSMTYANVTFTPSAADAKQRRMLIERTVAIRNQLP"
     gene            2887..3408
                     /locus_tag="VEW08_05335"
     CDS             2887..3408
                     /locus_tag="VEW08_05335"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HYJ40191.1"
                     /translation="MNSRQRGTTLVVGLILLTLVTLLGLAGGATAHIEVQLAHNEQFR
                     ENAASAASAGVEVAIGRIVTMPDPASATFSASAALPGAASRFEIQVRFLGYDIALPQP
                     AGANLAGAHFEIVSTGYSSRRAIDRQRAIVMRVDAAAGPAAASECEPMIPGVHCHEAG
                     ELVRVSWQRLPSP"
     gene            3405..3839
                     /locus_tag="VEW08_05340"
     CDS             3405..3839
                     /locus_tag="VEW08_05340"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HYJ40192.1"
                     /translation="MKVRFTLAELLTAVVGLVVIAAIAVPMWRTHQLRIRRVDAADAL
                     RAVQSAQDEYFGKHARYADEAQLRTSAPQAMSGNALSARGYYRITLEKSDDELTYSAV
                     ARAVPLDDEIADTRCVELRIDQNGRRYAVDATGEDRSADCWR"
     gene            complement(4594..6027)
                     /locus_tag="VEW08_05345"
     CDS             complement(4594..6027)
                     /locus_tag="VEW08_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011788495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HYJ40193.1"
                     /translation="MSARPTFREGFRGVTFAVSLALGACTMAPRYEQPAAPVPAEFEH
                     TDVGSTAVPVAPPAGDIGWKEFFPDPELQDLLARALANNRDLRIATLNVEAARAQYRI
                     QRADLMPAIDAQGSSTNARVPASLSQTGQSELSRTYSAGFGVSAFELDLFGRVRSLRR
                     AALEDYFRLEETRTAAQLVLVSEVANAWLTLIADRELLRLALETRDSQRKSYDLTKMR
                     FDAGVSSEMDLHRSETPWREAEVDIAQQTRRVAQDRNALALLVGEPLRADIASGEHAI
                     DAQTFSKTLPAGIPAELLVRRPDVRAAEHALKAANADIGAARAAFFPSIELTGFYGQA
                     SDDLSALFQSGHTAWSFTPQVHLPIFSGGRNLATLDLANVRKRIEIAHYEQSIQIAFR
                     EVADALVTRATLDDQLRAQEALTRASDSSYRLADQRYRGGVDSYLSALIAQRDLYTAQ
                     RQLIVTRLAGAVNLVRLYQAMGGGWKE"
     gene            complement(6024..9185)
                     /locus_tag="VEW08_05350"
     CDS             complement(6024..9185)
                     /locus_tag="VEW08_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYJ40194.1"
                     /translation="MLSRFFIDRPIFAWVIAIIVMIAGAVSVVNLPVRQYPAIAPPSV
                     GINVNYPGASAQTVQDTVIQVIEQALNGIDNLTYITSEANSDGSAVITLTFAQGTVPD
                     VAQVQVQNKLQTAMPLLPQEVQQAGIRVNKPARNFLVVMGFISADGSMNNEDLTDYVA
                     TNILDPLNRTSGVGDVTMFGSQYAMRIWLDPAKLNNYGLTTSDVVAAIRAQNVQVSVG
                     SIGGLPASPGQAISASIIGPTRMSAPEQFRDILLRVNADGSQVRIGDVARVALNSESY
                     TRDTKYNGKPAAGVAIRLAAGQNALDTVNAIRATIDRLSPFFPRGLEVRYPLDTTPFV
                     RTSILEVVKTLVEAVVLVFLVMYLFLQNARATLIPTIAIPVVLLGTFGVLAAFGFSIN
                     TLTMFGMALSIGLLVDDAIVVVENVERVMAEEGLAPREATRKSMDQITGALVAIALVL
                     GAVFVPMAFFSGSIGVIYRQFSITLVSAMSLSVLVALILTPALCATILKPLPPSGPVE
                     HKGLFGWFNRTFDAGRARHERGLAGMLKRPRRSMLAYVAIVVVMGLLFTRIPSSFLPA
                     EDPGFANGQVTTPPGASKERTWAALDQAQKYLLEHEKDAVEGVLTVNGFNFAGPGQNS
                     GLLFIKLRDWDDRKKSELSVSAVLARANKYFAGIKDANVFAIPPPAVLELGNTAGFDL
                     MLQNRGGLDRAQFLAARNQLLGAAAQDKRLAGVRPNGVEDAPQFRLDIDREKASALGV
                     SVADINSTLQTGWASTYVNDFIDRGRVKRVYVQGNPESRMQPDDLNRWYVRNKNGGMV
                     PFSAFGRGEWTYGPQKLVRFNGVPAYQIQGGPAPGTSSGEAMQAMEELVAKLPAGIGL
                     EWTGLSYEEKASGSQAPLMYALSLAIVFLCLAALYESWSIPATVLLVVPLGVLGAVIA
                     TLARGLTNDAYFQVGLLVTIGLAAKSAILIVEFAKENFDHGMDLVEAVMHATRQRVRP
                     ILMTSLAFMAGVLPLAISSGAGSGAQHAVGTGVIGGMLASTFLAVFFVPVFFVVILRY
                     FKVKPVTMKVDETPADVPYAGAT"
     gene            complement(9231..10415)
                     /locus_tag="VEW08_05355"
     CDS             complement(9231..10415)
                     /locus_tag="VEW08_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ40195.1"
                     /translation="MLNRNSACGAAGLLVAAVMLVACGKEAGGPPPPPEVGVITLAPR
                     AIAITDQLPGRTTAYRVAEVRPQVTGIVQKRLFVEGSEVKAGEQLFQIDAGSYRAAYE
                     SAAAALKRAEAQAVTAKLLEERYAPLIAANAVSQQENDDAIAARARADADVAAARAQV
                     ANARINVVYTQVLSPITGRIGRALVTEGALVTSAQESPLATVQQLDPIYVDITQSSTE
                     MLRLQRQVASGELTKNDNNEAEVGLTLEDGTLYAERGRLKVSEVSVDPSTGSVLLRAV
                     FPNPKRELLPGMFVRAQLTRGTRSAALLVPQRGVSRNAKGEATVLVVDKEDMVSERVV
                     TADRAINGEWLITAGLAAGERVVLDGLQKVKPGAPVKAVPAAEQMDAAAHASAAAEVA
                     KR"
     gene            complement(10399..10893)
                     /locus_tag="VEW08_05360"
     CDS             complement(10399..10893)
                     /locus_tag="VEW08_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYJ40196.1"
                     /translation="MSATARRIRDNTDPVPPAEDDVHRMICRQLKFVWSSLIRRVDLH
                     MQPLELTGMQWEPVLMLWLKRADTVAGLARVSQMGVASMTRMLDRLEEKELLRRERSE
                     LDRRVVNLHLTPKGKKAASKIWPIVVEGMHVHLDGFKKEELALLNDLLGRMLANGARD
                     AEQK"
     gene            11214..11678
                     /locus_tag="VEW08_05365"
     CDS             11214..11678
                     /locus_tag="VEW08_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ40197.1"
                     /translation="MTRREISILALGLTAGAIATVFAAESARPAPLASGVYAWASLPV
                     EKTASGERRAVFDSTTATVDRLETHITTIDEGSASHAAHRHPDEELIFVREGVVEATI
                     NGTTQLAPAGSIIFYASNDLHGMRNAGKGQASYFVLRWTSPGKEGPKPGGSR"
     gene            11675..12934
                     /locus_tag="VEW08_05370"
     CDS             11675..12934
                     /locus_tag="VEW08_05370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ40198.1"
                     /translation="MSQAAADQAAPGLDRRMLLLMCAAAAVLMVTMGMRQSLGLYIAP
                     IIESTHVGYAAMSFALAVGQLMWGVAQPVFGALADRHGSRPVLIAGALLLAGGTALTP
                     FAGNEFALLLTIGVLTAAGAGAGSFSVLIGVVAQRVPAAKRSFASGFVNAGGSLGQFV
                     FAPLNQLLIATFGWMVAMWTMAVAALATIPIAGPLLVRRGAGEARGAANVGGVAPSAL
                     SLREQLAIASRDRSYWCLHAGFFTCGFHISFLVTHLPGEVKLCSLPASVAAGSLAIIG
                     LANVAGSLTAGWAGGVMRMKLLLFAMYFSRAVAVAVYLAAPKTAVTFYLFAAVLGFTW
                     LATVPPTAGLIGKLFGVRYLATLFGLATFTHQIGGFFGAWLGGKALTAFGDFSWMWYA
                     DIALALAAAFINLPIREAKLNPVPAPA"
     gene            complement(12983..14260)
                     /locus_tag="VEW08_05375"
     CDS             complement(12983..14260)
                     /locus_tag="VEW08_05375"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HYJ40199.1"
                     /translation="MKNPLSRRAFLETSLFAGASTLMASRLAFASAPTEARFVFVLLR
                     GALDGLSAVPPVGDSDYAGLRGQIALAKSGEGAALPLEGPFALHPALEFLHRSYAAKE
                     LAVLHAVATPYRERSHFDAQNVLESGDLRPHGSTSGWMNRALTALPAGKSREAGVALG
                     ANVPLAMRGPAEVASWSPTKIATLDEGTLARVADLYARDPLLSQRLADALESDAIANE
                     AQAAAQAEPELDAQMKNDVVARDAKEKRNYGARYAETARAAAGFLKRDDGPRVAMFDT
                     TGWDTHANEGGAQGQLALRLRGLDAALAALKESLGPVWRNTVVLVATEFGRTAAINGT
                     RGTDHGTGAAAFLLGGAVAGGRVLADWPGLARANLLEARDLKPTRDLRTVMKGVLRDH
                     LDVPADALDTHVFPNSAAARPMDGLIKGSGTVS"
     gene            complement(14277..15695)
                     /locus_tag="VEW08_05380"
     CDS             complement(14277..15695)
                     /locus_tag="VEW08_05380"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HYJ40200.1"
                     /translation="MSETQKPSAAAIAANRFGLGARPGELPGIGVDAAGWLLRQLKGA
                     PPVLKGEGLKPSAETLAKVIELRKNKKEASDEQEKVAAALKLPAIYRPVYIDEVHARF
                     AHSVATERPFLERLTQFWTNHFAVSVDKNVVLGLAGAMEREAIRPHVAGHFTDLLLAV
                     EKHPAMLLYLDNQTSIGPNSKAARFVGKRGNGRKVGINENLAREILELHTLGVDGGYT
                     QADVTTFAQAISGWSIGGQDNGRRLAKLGVDNGTPGEFLFREVFHEPGAKKLLGKGYG
                     DDGVRQGESMLRDLAQRRETARHISTKLARHFIADVPPKAAVDRITRAWLDSKAHLPT
                     VYQALVESPEAWEQPLAKFKTPADYIYSSYRALGIPLREKRRALQPFEALGQRSLSPG
                     SPAGWPDASADWDGSSALLKRIAWADVVAQRMGDARNARDLAPQLLGATLTDDTAKAI
                     ARAESGAQALTLLMASPEFMRR"
BASE COUNT         2655 a         5234 c         5089 g         2796 t
ORIGIN      
        1 tttccctcca ccacgtgacg gcggtaggga atgacgcgcc aggcaatgaa cgcgatgaat
       61 cgcgcgaaca gataccagag ggcgagcggg atgcgcgaga acacgcgaat ccacatggaa
      121 gatttggggg cggagttcac ctagcaactt tcctcaaggg cgcgatcctg caactcatag
      181 aaaaggccgc atctagcggc ctttcgtctt ggatctggtg ccctctatct gattcgaaca
      241 gatgacccct tcattacgaa tgaagtgctc taccagctga gctaagaggg cttatcaatc
      301 ggtcactcac gccatgagct accccggcgt gagggcgcgc attctaaatg cgacgggagg
      361 gctagggaag ctctgaaacg gcggcggggg tgcccgaacc gggcgtcagg cttcggttct
      421 gcgacgcagt cggataacga ggtcgatgcc gtcgacctgc atgcccggcg gcggctcggg
      481 cagccgctgg aactcgacgt cgctcaggga cacatccatc agcgcaccgg aatcgagatc
      541 gtggaagtgg tagtgcgcgt cggtgttcga atcgaaccag cagcgatcgc tgcccacgga
      601 aagctggcgg atgaggccag aggccgcgaa cagattcaga gtgttgtaga tcgtggcctt
      661 ggaaacgcgc aaaccgagcc cgcgcaggcc cgccaggacc tgatcggcag aaaggtgttg
      721 gggtcgcccg agaagcagcg aggcaatgcg caaccgttgc gcggtgggtc gtacgccatg
      781 acccaacagg cgctggttca ggttctcgac taggcccgga tccagatccg acattggcat
      841 gggcgcgagt atacgcgccg gaatcctgcg ggcaacgcga aacgccgcaa acacggcggt
      901 tttgcgaggc tgcccacaga cgcgaggcgg aacagaatcc acgccatgtg catcaagaac
      961 cgcgaccggg gtgtcaccct ggtcgagctg tgtttcgggc tggccatcgt ggcggtcatt
     1021 gccgggttgg ccgcgcccgg ctttcggtcg acccttcggg ccgccgcggt tcgttccgcc
     1081 acgttcgacc tgctggccgg cctgcaaacg acacgcggca acgccatcct ggagtcgcgg
     1141 cccctggacc tgtgcccgag cgactccacg ggcaaatgcc ttgccgcagg cgtgcccacc
     1201 ccttactggc gatcgtccct tgaagcgggc gctgctcatt cgctccgcgc cagccatgtg
     1261 ttgcccgcgg gcgtggtggt tcgcgcctcc cgttcgccac tctggttctc gcccgacgcg
     1321 ctgggcgcaa gcactggaac actaactatc tgcgaccagc agggcgtggc tccaccacgc
     1381 gccatcgtgt tgagccagag cggccgcgca cgcctggaag ccgcgcacgc agaggcgtgc
     1441 caatgaaccg cacaacgcgc ggcttcaccc tgctcgaaac gctggtatcc ctcgtggtgc
     1501 tgtcggtggg cttgttaggc gcggccgccc tgctgctcga caacctgcgc acccatgccg
     1561 gcgcgctgcg ccgggtcgct gcgctgagcc tcgtgcgcga catggccgat cgcatccgcg
     1621 cgaatccacg cggcggcgtt tactacgaca cgagaatgcc ggcgccggcc gcggcgccat
     1681 gcggtgggcc gggcggctgc gacgtcgcac agctcgcggc cgcagacctc gcctacttcg
     1741 aatccacggc gcacgccctc tttccgcgtg ccgacaccaa cacacgagtg gaatacgcgc
     1801 ccgccaccgg ccacgccacg cctgcccgtc acatcattac gttgcgctgg tccgatgcgc
     1861 gcaatgacgc cggcaccgac agtgtcgcgc tgcaggttct gacgctgtcg ccggtggcgg
     1921 gctgagcgtg cgccgcccat cacgcgccgg cggcttcaat ctacccgagc tcatgatctc
     1981 gctgacgctt gggttgttcc tcgtggctgc cttcctcctc gtcctgcagc gttgccgcgc
     2041 ctccttcgcc gccaatgaaa gtctcgcgcg cctgcaggat gccgcgcgac atgcgttttc
     2101 ggtgctggtc ccggacatcg aacacgccgg gttctacggc ttcagccacg cgcgcgccgt
     2161 tcggctcgtc ggcagccttc cggccggcgc cgttgcctgc ggcacgaatt tcgccttgtc
     2221 gttgtccgtt cccgtgcaag gctcggacaa ccgctttcgc ctcggcgcgg acgcgcgcga
     2281 ctgcgcacct accgcatccg cgggcggggc gcgcgctgaa accgacacac tgaccttgcg
     2341 ccatgcgtcg ttgagtccgg tggcgccgcg agcgggacgg ctgcagatct actcgcaaaa
     2401 tctttcgatc gcgacgcccc cgctgctgtt tgcggacggc cgcgcgccgg tgcccgtgga
     2461 tccggatcat gtggttcacg atctcgaggt tcgcacttac tacatcgcaa acgactcggt
     2521 gcagcggcca gggtggcccg cgttgcgtgt gaaggcactg acggagtcgc gtggcgccgc
     2581 gcagttccgc gatgaagaaa tcctgcccgg cgttgaagac ctgcagatcg agatgggcgt
     2641 cgcctcgatt gccgccgatg gaatcccgcg agtgacctac gttgggccgg attctccgca
     2701 ggcacgagac ggaaacgtgg tggcagtcag gttctggctg cggattcgcg ccgacgccac
     2761 cgaacccggt ttcagcgacg gccgcagtat gacgtatgcc aacgtcacct tcacaccgtc
     2821 ggccgccgac gcgaagcagc gccgcatgct catcgagcgt acggtcgcaa ttcgtaatca
     2881 gctgccatga actctcgtca acgcggcacc acactggtgg tcggcctcat tctgctgacg
     2941 ctggtaacgc tgttaggact tgccggaggc gccaccgctc acatcgaagt gcagctggca
     3001 cacaacgagc agtttcgcga gaatgccgcg tccgccgcca gtgccggcgt cgaagttgcc
     3061 atcggcagaa tagtgaccat gcccgatccc gcctccgcca cgttctccgc cagcgcggct
     3121 ctgccaggcg ccgccagccg ttttgaaatt caggtccggt tccttggcta cgacatcgcg
     3181 ctgcctcagc cggccggcgc caatctcgcc ggcgcgcatt tcgaaatcgt cagcaccggt
     3241 tactcgtcac gacgtgcaat cgaccgccag cgtgccattg tcatgcgcgt cgacgccgct
     3301 gccgggcccg cagcggcttc agagtgcgag ccgatgatcc ccggcgttca ctgtcatgaa
     3361 gccggcgaac tggtgcgcgt gtcctggcag cggctcccct cgccgtgaaa gtcagattca
     3421 cactggccga gctgctcacc gccgtcgtgg gtctggtggt catcgccgcc attgcggtac
     3481 ccatgtggcg cacgcaccag ttgcgcatcc gccgggtgga cgccgcggat gcccttcgcg
     3541 cggtacagtc tgcgcaggac gaatactttg gcaagcatgc gcgctacgcc gacgaagcgc
     3601 agttgcgcac cagcgcgccg caggcaatgt caggcaatgc gttgtccgcg cgtggctatt
     3661 accggatcac gcttgaaaag agcgacgacg agctgaccta ctcggccgtc gcgcgcgccg
     3721 ttcctctcga cgatgagatt gcggacacgc gctgtgtgga gctgcgcatc gaccagaatg
     3781 gccggcggta tgcggtggat gccaccggag aagacaggag cgcggactgc tggcggtaaa
     3841 tggggacatt cctcgtttcc tggcaaaagg ggacggggct catgggtgga gggtcgagcg
     3901 acgggcggcc ctgcatgcga tgagactcgc ttttcgccgg gcccttcgtt cagggtgaca
     3961 tcgctagttt gcgggccgcc aacacacgcg aagacatctt gtagtaagtt gagttgggcg
     4021 gcagcaaaga gtcctgaagg cgcagtgatc ggcttcgtgc ctagcgaggc caggtttccc
     4081 ggcccgctcg ctcatcgcat gcagggccac ccgtcgctcg atcgtgcacc gacgcgccgc
     4141 gactccgttc gtcagaaaaa gaggcgtgta accccacgcg gtgggaagac ctcaagtccg
     4201 tgcgtctctt ctcttaccac cgccgcggga aagcgggcgc ggttcggtga cgtatgagcc
     4261 ccagcgagca gtccgcgcac aggagctgag agagcgggcc gggatacctg gcctcgttag
     4321 gcacgaagcc gcctctccgc ttcattcgga ctctcgctga ctttccaccc caaccgcacg
     4381 aaagtgtcat tgcgtgtggc ggcggcccgc aaactagcga tgccaaccca agcgaagggc
     4441 ccggcgaaaa gcgaacgaaa tcctgtgcgc ggaatgctcg ctggggcacg cacaccccca
     4501 aaccgcccct ctttacgaaa ccccagcgaa aagcgaacga aatcctgcgc gcggactgct
     4561 cgctggggcc catgcccatc tatccaaccc ctcctattcc ttccacccgc cacccatcgc
     4621 ctgataaagc cgcacaagat tcaccgcccc cgccagccgc gtcacgatga gctgccgctg
     4681 cgcggtgtac aggtcgcgct gcgcgatcag cgcgctgaga tagctgtcca caccaccgcg
     4741 gtatcgctgg tccgccagcc ggtagctcga gtcactggcg cgcgtcagcg cttcctgcgc
     4801 gcgcaactga tcgtcgagcg tggctcgcgt aaccagggca tcggccactt cgcgaaatgc
     4861 gatctggatc gattgctcgt aatgcgcaat ctcgatgcgc ttgcgcacgt tggcgagatc
     4921 gagcgtggcg agattccggc cgccggaaaa aatcggcagg tggacctgcg gcgtgaaact
     4981 ccaggcggtg tgcccggact gaaacaatgc cgagaggtcg tcgctggcct gcccatagaa
     5041 gccggtgagc tcgatgctcg ggaagaatgc cgcgcgagcg gcgccgatat cggcgttggc
     5101 ggccttgagc gcgtgttccg cggcgcgcac gtcgggccgg cgcaccagca attcggccgg
     5161 aatgccggct ggcagtgtct tcgaaaacgt ctgcgcatcg atggcgtgtt cgccggaggc
     5221 gatatcggca cgcagcggtt ctcccaccag cagcgccagc gcattgcggt cctgcgccac
     5281 gcgccgggtc tgctgcgcaa tgtccacttc cgcctcgcgc cagggagtct cggatcgatg
     5341 cagatccatc tcgctcgaca cgcccgcgtc gaagcgcatt ttcgtcaggt catatgactt
     5401 gcgctggctg tcgcgcgttt cgagcgccag ccgcagcaac tcgcggtcag cgatcagcgt
     5461 gagccaggcg ttggcgactt ccgacaccag caccagctgc gccgcagtgc gcgtctcctc
     5521 gagccggaag tagtcctcca gcgccgcgcg gcgcaggctg cgcacgcggc cgaacagatc
     5581 gagttcaaaa gccgacacgc cgaaaccagc ggaatacgtg cgcgaaagct cactctgccc
     5641 ggtctgcgac aagctcgccg gcacgcgcgc gttggtcgac gaaccctgcg cgtcgatggc
     5701 cggcatgaga tcggcgcgct ggatgcggta ctgcgcacgc gccgcctcga cgttgagcgt
     5761 ggcgatgcgc aggtcccggt tgttagccag tgcccgcgcc agcaggtcct gaagttcggg
     5821 gtcgggaaaa aattctttcc agccgatatc gcccgcgggc ggtgctacgg gtacggcggt
     5881 cgagccgacg tcggtgtgct caaattccgc cggcaccggc gccgccggct gttcataacg
     5941 aggcgccatc gtgcaggcgc cgagcgccag cgagacagcg aacgtgactc cacgaaaccc
     6001 ttcgcgaaag gtcgggcgcg cgctcacgtg gcacccgcgt agggtacgtc ggccggtgtt
     6061 tcatccacct tcatggtcac cggcttcact ttgaagtaac gcaggatcac cacgaagaat
     6121 accggcacga aaaacaccgc caggaacgtg gaagccagca tgccgccgat cacacccgtg
     6181 cccaccgcat gttgcgcgcc ggaaccggcg cccgaggaga tggcgagcgg caaaacgccg
     6241 gccatgaacg cgagcgaggt catcaggatc ggacggacgc gttgcctggt cgcgtgcatc
     6301 accgcttcca ccaggtccat gccgtggtcg aaattctcct tcgcgaattc cacgatgagg
     6361 atcgcgctct tggcagcgag tccgatcgtg accagcaggc ctacctggaa gtaggcgtca
     6421 ttcgtgagtc cgcgcgccag cgtcgcgatg acggcaccga gcacgccgag cggcaccacc
     6481 agcagcaccg tcgccgggat cgaccagctc tcgtacagcg ccgcaaggca caggaacacg
     6541 atcgcgagcg acagcgcgta catcaggggc gcctgggagc cggacgcctt ctcttcgtac
     6601 gacaggccgg tccattccag accgatgccc gcgggtagtt tggcgacgag ctcttccatg
     6661 gcctgcatcg cctcgcccga acttgtgccc ggcgccggtc caccctggat ctgataggcc
     6721 ggcacgccgt tgaagcgcac gagtttctgt ggtccgtagg tccactcgcc gcggccgaac
     6781 gccgagaacg gaaccatgcc gccgttcttg ttgcgcacgt accagcgatt gaggtcatcc
     6841 ggctgcatgc gcgactcggg attgccctgc acgtacacac gcttgactcg gccgcgatcg
     6901 atgaagtcgt tgacataggt agacgcccaa ccggtctgca gcgtcgagtt gatgtccgcc
     6961 accgacacgc cgagcgcgct cgccttttca cgatcgatgt cgagcctgaa ctgcggcgcg
     7021 tcttcgaccc cgttcgggcg cacgcccgcc aggcgcttgt cctgggcggc ggcccctaac
     7081 aactgattgc gcgccgcgag aaactgggcg cgatcgagcc cgccacggtt ctgcagcatg
     7141 agatcaaagc cggcggtatt gccaagctcg agcacggccg gcggaggaat cgcgaagaca
     7201 ttggcgtcct tgatgccggc gaaatatttg ttggcgcggg cgagcacggc cgaaactgac
     7261 aactcgctct tcttgcgatc atcccagtcg cgcagcttga tgaagaggag gccggaattc
     7321 tggcccgggc ccgcgaaatt gaaaccgttg acggtgagta cgccttcgac cgcgtccttc
     7381 tcgtgttcga gcagatactt ctgcgcctga tcgagcgctg cccaggtacg ctccttggaa
     7441 gcgccaggag gcgtcgtcac ttgtccgttg gcgaagcctg gatcctcggc aggcaggaac
     7501 gacgacggaa tacgcgtgaa cagcaagccc atgaccacca cgatggcaac gtacgcgagc
     7561 attgaacgtc gcgggcgctt cagcattccc gcgaggccgc gttcatgacg cgccctgccc
     7621 gcgtcgaaag tacggttgaa ccagccgaac aaacccttgt gttccacggg tccggacggc
     7681 ggcagcggct tgaggatcgt ggcgcacagc gcgggcgtga ggatcagtgc cacgagcacc
     7741 gagaggctca tcgccgacac cagcgtgatg gagaactggc ggtagatgac gccgatggag
     7801 ccactgaaga acgccattgg cacgaatacc gcgcccagta ccagcgcgat ggcgaccagc
     7861 gcgcccgtga tctggtccat ggatttgcgg gtggcctcgc gcggcgcgag cccctcctcg
     7921 gccatgaccc gttcgacgtt ttccacgacc acgatggcgt catcgaccag caggccgatg
     7981 gagagcgcca tgccgaacat cgtcagcgtg ttgatcgaaa atccgaacgc ggcgaggacg
     8041 ccgaatgtgc ccagcaacac caccggaatg gcgatggtag gtatcagcgt ggctcgtgcg
     8101 ttctgcagga acaggtacat gaccaggaac acgagcacca ccgcctcgac gagcgtcttc
     8161 accacttcga ggatcgaggt cctgacgaac ggcgtcgtgt cgagcggata cctcacctcg
     8221 agaccgcggg ggaagaacgg cgacaggcga tctatcgtcg cgcgaatggc gttgaccgta
     8281 tcgagtgcgt tctgccccgc cgctagccgg atcgcaacgc cggccgcggg cttgccgttg
     8341 tacttggtgt ctcgcgtgta actttccgaa ttgagcgcga cgcgcgcgac gtcgccgatg
     8401 cgcacctgcg acccgtccgc attgacgcgc agcaggatgt cgcggaactg ttccggcgcg
     8461 gacatgcgcg tcggcccgat gatgctcgcg ctgatcgcct gtcctgggct ggctggcaag
     8521 ccaccgatcg aacccacgga aacctgcacg ttctgcgccc ggatcgcggc gaccacgtcg
     8581 ctggtggtga ggccgtaatt attgagcttc gccggatcga gccagatgcg catcgcatac
     8641 tgcgaaccga acatcgtgac gtcgccgacg cccgacgtgc gattcagcgg atcgaggatg
     8701 ttcgtcgcga cgtagtcggt caggtcctcg ttgttcatgc tgccatcggc ggagatgaag
     8761 cccatcacca cgagaaagtt gcgcgccggt ttgttcacgc ggatgccggc ctgctggact
     8821 tcctgtggca gcaacggcat ggcggtctgc agcttgttct gtacctgaac ctgggccacg
     8881 tcgggcaccg tgccctgcgc aaacgtcagc gtgatgaccg cggagccgtc ggagttggcc
     8941 tcggaggtga tgtacgtaag gttgtcgatg ccgttcaagg cctgctcgat gacctggatc
     9001 accgtgtcct gcaccgtctg cgcggaagcg cccgggtagt tcacgttgat gccgaccgac
     9061 ggcggagcga tggccgggta ctgccgcacc ggcaggttca ccaccgagac ggcgcccgcg
     9121 atcatgacga tgatcgcgat gacccacgcg aagatgggcc tgtcgatgaa aaatcgggac
     9181 agcatgacta ctcgtttatg cctgggttga tgcctgggtt gatgcccggg ttaccgcttc
     9241 gcgacttcgg cggcagcgct cgcgtgcgcg gcggcgtcca tctgctcggc cgcgggcact
     9301 gccttcacgg gcgcgcctgg cttgaccttc tgcagaccat cgagcacaac gcgctcgccc
     9361 gcggcgaggc ctgcggtgat cagccactcg ccgttgatgg cacgatccgc ggtgaccacg
     9421 cgctcgctca ccatgtcttc cttatccacc accaggacgg tggcctcgcc cttcgcgttt
     9481 cgcgagacgc cgcgctgcgg cacgagcagc gccgcggagc gagtgcctcg cgtgagctgg
     9541 gcgcgcacga acatgccggg cagcagttcg cgcttcggat tcgggaaaac ggctcgcagc
     9601 agcaccgagc ccgtgctcgg atccaccgac acctcggaga ccttgaggcg accgcgttcg
     9661 gcgtacaacg taccgtcttc gagcgtcagg ccgacttcgg cctcgttgtt gtcattcttc
     9721 gtcaactcgc cgctcgcgac ctggcgctgc aggcgcaaca tctcggtgga ggactgcgtg
     9781 atgtccacgt agatagggtc aagctgctgc acggtggcga gcgggctctc ctgcgcggaa
     9841 gtgaccagcg cgccttcggt caccagtgca cgtccgattc gcccggttat gggcgacagc
     9901 acctgcgtgt acaccacgtt gatgcgtgcg ttggcaacct gggcacgcgc ggcggcaacg
     9961 tcggcgtcgg cgcgcgcacg cgcggcgatg gcgtcgtcgt tctcctgttg ggacacggca
    10021 ttggccgcga tcagcggtgc atatctttct tcgaggagct tcgcggtgac cgcctgcgct
    10081 tcggcgcgct tgagtgccgc ggcagcggac tcgtacgcgg cgcgataact gccggcgtcg
    10141 atctggaaca actgctcgcc cgccttgact tcgctgcctt cgacgaacag gcgcttctgc
    10201 acgatgcccg tgacctgtgg ccggacttcg gcgacgcggt acgcggtggt gcggccgggc
    10261 agctggtcgg tgatcgcaat ggcgcgcggt gcgagcgtga tcacgcccac ttccggcggc
    10321 ggcggtggtc cgcccgcctc tttgccgcag gccacgagca tgacggccgc caccagcagg
    10381 ccggcggctc cgcaggcgct atttctgttc agcatcgcgc gctccattcg ccagcattcg
    10441 gcccaacaag tcgttgagca gggcgagttc ttctttcttg aatccatcga gatgtacgtg
    10501 cattccttcg accacgatcg gccagatctt cgaagcggcc ttctttccct tgggcgtgag
    10561 gtgcaggttg accacgcgcc ggtcgagttc gctgcgttcg cggcgcagca gttccttctc
    10621 ctcgagccgg tcgagcatcc gcgtcatcga ggcgacgccc atctggctca cccgcgccaa
    10681 tcccgcgacc gtgtccgcac gtttgagcca caacatgagc acgggttccc actgcatgcc
    10741 ggtgagttcg agcggctgca tgtgcaggtc cacgcgacgg atcagcgagc tccagacgaa
    10801 cttgagctgc cggcagatca tgcgatgcac gtcgtcttcg gccgggggta ccgggtcggt
    10861 attgtcgcgt atgcgtcggg cggtggcgct catgggtttt cggtcgattg gccagggagg
    10921 cgcgtagctt tcagtggcct tgcaatagtt gtcaaggcaa atattgcagg ggcaatctgt
    10981 cgacgaaagt cgtatgtgcg ccgcggtcag accctgaggt gtaacaaaag gcgctacaag
    11041 ctggctgata cgcaagggga tttccgtcag acgacgccgc gcccgcctcc ggcatgcaaa
    11101 agcaccgctt aaccgtgcga tagaaatccg cgtgaagtgt ctctggctta gaggatgccc
    11161 gtcgccgatc atccgtatcc ccacaggcca gcaacgcaaa cgggagcatc cggatgacgc
    11221 gtcgtgaaat ttccattctc gccctcggtc tgacggccgg cgccatcgcc acggtattcg
    11281 cagcggaaag cgcaaggcct gccccgctcg cctcgggcgt atatgcctgg gcatcgctgc
    11341 ccgtggagaa aaccgcgagc ggcgaacgcc gcgcggtctt cgattccacc accgccacgg
    11401 tcgatcgcct cgagacgcac atcaccacga tcgatgaggg atcggcttcg catgcagccc
    11461 atcgtcaccc ggatgaagaa ctgatcttcg ttcgcgaagg tgtggtcgaa gcgaccatca
    11521 acggcacgac tcagctggcg cccgccggct cgatcatctt ctatgcctcg aatgatctgc
    11581 acggcatgcg caatgccggc aaaggccagg cgagttactt cgtactgcgc tggacctcgc
    11641 ccgggaagga aggccccaaa cccggcggtt cacgttgagc caggcggcgg ctgaccaggc
    11701 ggcgccggga ctcgaccgtc gcatgctgct gttgatgtgc gcggcggcgg cggtgctcat
    11761 ggtcaccatg ggcatgcgtc agtcgctcgg gctgtacatc gcgccgatca tcgagagcac
    11821 gcatgtcggt tatgcggcca tgagcttcgc gctggcggtg ggccagctca tgtggggcgt
    11881 cgcgcagccg gtgttcggcg cgctggcgga tcgccatggc tcgcgaccgg tgctcatcgc
    11941 gggcgcgctg ttgttagccg gcggtacggc gctgacgccg ttcgccggca atgaattcgc
    12001 attgctgctc accatcggcg tgctcacggc agccggtgcc ggcgcgggca gcttttcggt
    12061 gctcatcggc gtcgtggcgc agcgcgtgcc ggccgccaag cgctcgtttg cgtccggttt
    12121 cgtcaacgcg ggcggcagcc tcgggcagtt cgtgttcgcg ccgctcaacc agttgctcat
    12181 cgcgaccttc ggctggatgg tggcgatgtg gaccatggcg gtcgccgcgc tggccaccat
    12241 ccccatcgcc ggtcccttgc tcgtacggcg tggcgccggc gaggcgcgcg gcgccgcgaa
    12301 cgtgggcggc gttgcgccga gcgcgctcag tctgcgcgag caactcgcga tcgcctcgcg
    12361 cgaccgcagc tactggtgcc tccatgcggg attcttcacc tgcggctttc acatttcgtt
    12421 tctcgtcacc cacctgcccg gtgaagtgaa gctctgcagc ctccccgcct ccgtggccgc
    12481 gggctcgctc gccatcatcg gcctcgcgaa tgtggccggc agcctcacgg cgggttgggc
    12541 cggtggcgtc atgcgcatga agctgctgct gttcgccatg tatttcagcc gcgccgtcgc
    12601 cgtggccgtg tacctggctg cgcccaagac cgcggtcacg ttctacctgt tcgccgccgt
    12661 gctgggtttc acctggcttg ccaccgtgcc gccgacggcc gggctcatcg gcaagttgtt
    12721 tggcgtgcgt tatctcgcga ccttgttcgg cctcgcgacg ttcacccacc agatcggcgg
    12781 gttcttcggc gcgtggctcg gcggcaaggc gctgaccgcc ttcggtgact tttcgtggat
    12841 gtggtacgcg gacatcgcgc tggcgctcgc cgcagcgttc atcaacctgc ccatccgcga
    12901 agcgaagttg aaccccgtgc ccgccccggc ctgaaatggg gtcaggcacc gtttgctaga
    12961 aaacggtgcc tggcaccgat tcctaggaaa cggtgcctga cccctttatc aatccatcca
    13021 tcggtcgcgc ggcggcgctg ttggggaaca cgtgggtgtc gagcgcatct gcaggcacat
    13081 cgaggtggtc gcgcagcacg cctttcatga cggtgcgcaa gtctcgcgtt ggcttgagat
    13141 cacgagcctc gaggaggttc gctcgcgcca ggcccggcca gtcggccagc acgcggccgc
    13201 cggcgacggc gccgcctaac aggaacgccg cagcgcccgt tccatggtcc gttccgcgcg
    13261 tgccattgat tgcggccgtt cggccgaact cggtcgcgac gagcaccacg gtgttgcgcc
    13321 acaccggtcc gagcgactcc ttcaatgccg ccaatgcggc atcgaggccc cgcagccgca
    13381 gcgccagctg accttgcgcg ccgccctcgt tggcatgtgt atcccagccc gtggtgtcga
    13441 acatggccac gcgaggacca tcgtcgcgct tgagaaatcc ggccgcggcg cgcgccgtct
    13501 ccgcgtaacg cgcgccgtag ttgcgctttt ctttcgcgtc gcgcgccacg acatcgtttt
    13561 tcatttgcgc atcgagttcc ggctccgcct gcgcggccgc ctgcgcttca ttggcgatgg
    13621 catcgctttc cagcgcgtcc gcgaggcgct gcgacagcag cggatcgcgc gcatacagat
    13681 ccgcgactcg cgccagcgtg ccctcatcga gcgtcgcaat tttcgtcggc gaccaggacg
    13741 ccacctcggc aggtccacgc atcgcgagcg gcacattggc gccgagcgcc acgccggctt
    13801 cgcgcgattt gcccgccggc aacgcggtca aagcgcgatt catccagccg ctcgtcgaac
    13861 catgcggacg caggtcaccg ctctcgagca cgttctgcgc gtcgaaatgc gatcgctcgc
    13921 gatacggcgt ggccacggca tgcagcaccg cgagttcctt cgcggcgtag ctgcgatgca
    13981 agaactcgag cgcaggatgc agcgcgaatg gaccctcgag cggcaatgcc gccccctcgc
    14041 ccgacttcgc gagcgcgatc tgtccgcgca agccggcata gtcggagtcg cccaccgggg
    14101 gcacggccga caagccgtcg agcgcgcctc gcagcagcac gaatacgaag cgggcttcgg
    14161 tgggcgcgct ggcgaacgcg agacgcgacg ccatcagcgt actggcgccg gcaaacagcg
    14221 aggtttcgag gaaggcgcgt cgcgacaggg ggttcttcat gtgcttctcc ggcgactcag
    14281 cgccgcatga actccgggct ggccatgagc agcgtcaacg cctgcgcgcc gctctcggcg
    14341 cgcgcgatgg ccttcgccgt gtcatcggtg agcgtggctc ctaacaactg gggcgccagg
    14401 tcgcgtgcgt tgcgcgcgtc gcccatgcgc tgtgcgacca catcggccca ggcaatacgt
    14461 ttgagcaacg ccgacgagcc atcccagtcc gcgctcgcat ccggccagcc agcgggcgaa
    14521 cccgggctga gactgcgctg gccaagcgct tcgaagggct gcaacgcgcg gcgtttctca
    14581 cgcagcggga tgccgagcgc gcgatatgag gaatagatgt agtcagccgg tgtcttgaac
    14641 ttcgcgagcg gctgttccca ggcctccggg gattcgacca gggcctggta gaccgtgggc
    14701 agatgcgcct tcgaatcgag ccaggcgcga gtgatgcgat cgacggccgc ctttggcgga
    14761 acgtcggcga tgaaatgccg tgcgagcttg gtcgatatgt gccgtgccgt ttcgcgccgc
    14821 tgggcgagat cgcgcaacat ggattcgccc tgccgcacgc catcgtcgcc gtaacccttg
    14881 cccaacaatt tcttcgcgcc gggttcgtgg aagacttcgc ggaacaggaa ttcgccgggc
    14941 gtgccgttgt cgacaccgag tttcgcgagc ctgcggccgt tatcctggcc gccgatcgac
    15001 catccggaaa tcgcctgcgc gaacgtggtc acgtccgcct gcgtgtagcc accgtccact
    15061 cccagcgtgt gcagctcgag gatttcacgc gccagatttt cgttgatgcc caccttccgg
    15121 ccattgcccc gtttgccgac gaaacgcgcc gccttcgagt tcggaccgat cgaagtctgg
    15181 ttgtccagat acagcagcat ggccggatgc ttctcgacgg ctaacaacag gtcggtgaag
    15241 tgacccgcga catgcggccg gatggcttcc cgttccatgg cgcctgcgag acccaggacc
    15301 acgttcttgt ccacggacac cgcgaaatga ttggtccaga actgggtcag gcgctcgagg
    15361 aacggccgct cggtggccac cgagtgagca aagcgcgcat ggacctcgtc gatgtacacc
    15421 gggcgataga tcgcgggcag cttgagcgcc gccgccacct tttcctgttc atcgctggct
    15481 tccttcttgt tcttgcgcag ctcgatgact ttcgcgaggg tctcggcaga aggcttcagg
    15541 ccttcgcctt tgaggacggg tggcgcgccc ttgagctggc gaagcagcca gccggcggca
    15601 tccacgccga tgccgggcaa ttctccgggc cgggcaccga gcccgaagcg gttggcggca
    15661 atcgccgcgg cggaaggttt ttgcgtctca gacatgggct tatttcccgc tcaacgcact
    15721 cgtctccttt cggttgacac gcagcaggcc gtcgacccag cgggccagtt gcgg
//
LOCUS       DASQJS010000129        14886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1322170, whole genome shotgun sequence.
ACCESSION   DASQJS010000129 DASQJS010000000
VERSION     DASQJS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14886
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322170"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1312)
                     /gene="nagA"
                     /locus_tag="VEW08_05385"
     CDS             complement(164..1312)
                     /gene="nagA"
                     /locus_tag="VEW08_05385"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HYJ40201.1"
                     /translation="MLLAFRNGKILTEAGIESGRCLLVRDGHIEAISGAREVVGADQT
                     IDLDGHLLAPGFIDTQVNGGGGVLFNGNPSVESIATIGRAHRRFGTTGFLPTLISDDL
                     HVVEQAILAVREAIARGVPGVLGIHIEGPFLSNERRGVHDASKLRVLDEDAMRLLATP
                     HGGVTMVTLAPERTTPAIIRALTDAGVIVSAGHTNATYDELQPALEAGVRGFTHLFNA
                     MSQLGNREPGAVGAALAHPDSWLGLIVDGYHVHAEVLKIALRAKRHDRFMLVSDAMPS
                     VGADVKEFRIQGRLITVDGDKIVDDEGRLAGAHLDMASAVRNTVNQLGVPLSDALRMA
                     SAYPAEFLKLGGRMGRIAPGQRADLVLVDEELTVLRSWIGGRADQLLA"
     gene            complement(1312..2337)
                     /locus_tag="VEW08_05390"
     CDS             complement(1312..2337)
                     /locus_tag="VEW08_05390"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HYJ40202.1"
                     /translation="MIAQTRMYQEAASAPQVVGAQLTHNEPLLLALGARLRKNPPRAV
                     VTCARGSSDHAATYAKYLIESHTHVLTSSAAPSVSSIYESQADLDGVLFIAISQSGMS
                     PDLLTATRNAREGGALTVALCNSPESPLMRIVDVAIPLHAGPEASVAATKSYIASLSC
                     IAQLLAHWTEDRKLLAALPQLPELLQRAWKCDWAPAVDALRGAVNLFVVARGYGLGVA
                     QEAALKLKETCGLHAEAFSSAEVKHGPMALVRAKFPVLMFTQRDDTRAGIEELAAEFA
                     ARGARVLLAGAEARGAVSLPTIEANPVIEPLLAIQSFYAMVDTLALARGFDPDRPPHL
                     NKITETV"
     gene            complement(2334..3077)
                     /locus_tag="VEW08_05395"
     CDS             complement(2334..3077)
                     /locus_tag="VEW08_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYJ40203.1"
                     /translation="MPTLSQVLPKLDESSSQPLYQQLQRALREAIENGVIGPDDALPP
                     ERDLADMLSVSRITVRKAIEELVEEGLLTRKQGSGTFVSNRVEKNFAKLTSFSEDMRA
                     RGREPRSVWLSRAEGTVTPEESLTLRSSPGTAVYRFSRIRYADDAPMALEYATVLRDC
                     LPSLEAVESSLYEALEQKGNRPVRALQRLRAVLLTAEQAKLLKAQERDAGLLVARVGY
                     LKDGRAVEFSQSYYRGDIYDFVAELSASS"
     gene            3273..3611
                     /locus_tag="VEW08_05400"
     CDS             3273..3611
                     /locus_tag="VEW08_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40204.1"
                     /translation="MKTELPNRFMSTTSLTLRANATPPATAKHVARKSGAEIETVVVP
                     GTHPSLESQTVIDVLTAYKNVAEALQRVSGASISREFGAGERASICGTAPRISGCLSA
                     FIRQDSVSLI"
     gene            3637..5763
                     /locus_tag="VEW08_05405"
     CDS             3637..5763
                     /locus_tag="VEW08_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HYJ40205.1"
                     /translation="MNKNQVSYLALSVVFGLAAGCGSPDATPAKPVQLDAPHLGLIPT
                     PAHSNGAVGTFRVAATTDVLFSGGDGASDVAQYFVELIKEQRPDLAFRKPREDTPSSG
                     SIAFVLAADEKSQGAEGYSLAVTPKGVTITARTPAGLFYGAVTLWQLITAEAMQGLSV
                     DIPALRITDAPRFAWRGVMVDSARHFQSPEYIKQFIDWMALHKLNVLHWHLVDDQGWR
                     LEIKKYPRLTSIGAWRVPAGQAPLTDIDTATGKPRQYGGFYTQDQVRDIVAHAAKRHI
                     TVVPEIEMPGHASAAVVAYPELGVTNKPPKAVPEEWGIFPTLFNIDDSTFTFLENVLA
                     ETIELFPSEYIHVGGDEALKDEWHASPKIQARMKELGIKDEHALQSYFIQRIGKYIDS
                     KGRKLIGWDEILEGGLAPNATVMSWRGIEGAIAAAKAGHDTVLSPAPDLYFDHWQSPG
                     DTSPGRSNTLSLKDVYLFNPLPAAIQPEQRKHVLGLQANLWAEFMRSEARVTYQAYPR
                     VAALAEVAWSPPEQINWDNFQKRLEPQLRRYATLGIGYARETPVVPGPRRRVSHDLEQ
                     CGGGYLLSLEDDAPLQGERAVFLVNITDPCWIWKGVDLSQVKAIRATVGQIPFNFQIG
                     ADAKKIPLHKPATKSGELEIRLDDCKGKVVASASLEPAVSNVGLTPLPPIEMPSQEGQ
                     HDICFTFTRAKVDPIWAIGSLELIGY"
     gene            5766..8240
                     /gene="ptsP"
                     /locus_tag="VEW08_05410"
     CDS             5766..8240
                     /gene="ptsP"
                     /locus_tag="VEW08_05410"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HYJ40206.1"
                     /translation="MPLIIRAPFAGWCAPLSEIPDAAFAQGLLGDGVGLDPTGNELRS
                     PCDGEVISVAASRHALALRTAAGAEILVHVGVDTVGLAGEGFNVHVRKGDRVHAGDLL
                     ITFDLDLLARKAPSLMTPVIVTNADRFRIAQANTRRKVASGDALFEIEQFAAGESGDT
                     GEPAVSGAVVVSESVVVDHAHGIHARPAAQIARIAKGLPFEIELRARGRGARARSTVA
                     LMSLGIRGGDEVVIVGFEPAAAVGIAAIVKAIRQLERVAPSAAVAPVAAAPVGDTSQL
                     RGVIASPGLGVGTAFYLHAAELPVTEAGRGVQQESTDFERARDAVRARLSRLATTAAP
                     TLREIMSAHLELLDDPDLVDGARAAIAGGKSAGFAWRESLHVSARALSATGDARLAER
                     VDDLRDLERQLLLALDGAQAPSFVIPPEGILLARDLTPSQLVDIDASKLAGIALAAGG
                     PTSHVAILAATLGVPMLVAMGPSLLEVSSGSKVILDADAGIMRADPDAQTLAETRRRL
                     ERGRALREQQTAAAQVDCHLASGERIEVFANLTGSEADATLAAAQGAEGCGLLRTEFL
                     FLERETAPDEEEQLRCYSQVARILGTRPLIIRTLDIGGDKPIPYLPLPPEDNPALGLR
                     GVRTSLWRPDLLEVQLRAMLRVQPAGVVRILLPMITDVAEIRAVRRLLDEACARLEVS
                     PPVLGAMIETPAAAVLADAIAREVDFLSIGTNDLAQYTLAMDRGHAELAARIDGVHPA
                     VLRMIDITCRGAAASGKPVAVCGGLAADPSAVPVLLGLGVSELSVVPTLIPQLKAQVR
                     ALTLEACRATAARALALESAAQVRAL"
     gene            8237..9982
                     /gene="nagE"
                     /locus_tag="VEW08_05415"
     CDS             8237..9982
                     /gene="nagE"
                     /locus_tag="VEW08_05415"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR01998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HYJ40207.1"
                     /translation="MNKIIGKLQPIGRALMLPIAVLPVAALLLRLGQPDLLDVPAIAA
                     AGDAIFSNLGLLFAIGVAVGLARENHGAAGLAAVVGYLVATQGAKVLIDVPPEVTAGI
                     PDQLKDMAAAAFRAKELSKLSVPVGLLSGLIAGWAYNRFSDIRLPSYLAFFGGRRFVP
                     IVSGFAGLGLALAFGFGWQWLESGMDATSQAVLGSESVGLFTYGVLNRVLIVTGLHHI
                     INNIAWFLLGDYQGVTGDLKRFFAGDPSAGAFMSGFFPVMMFGLPGACLAMYRTATPA
                     RRAAAGGLLLSMALTSFLTGVTEPIEFAFMFLAPVLYVIHAVLTGIAMVLMHALDVHL
                     GFGFSAGLFDYVLNYSLATRPLMLIPVGLGYFALYYFLFRYCIERFNLKTLGREADGA
                     AVATVAAGSETGPAAGWIRGLGQAANLRSVEACTTRLRLVLNDPSLIDEAALTALGSR
                     GVLRIGDGAVQVVVGPIADQLASEIRAQWRAPTPAVSAPAPVPSPAPALAAAPDRSIL
                     AGVLAALGGRGNITGMHANSTRLVISVRDPAAVDESALGRLANAIARPAPASLHLIVG
                     PEARSWLAVMETLTS"
     gene            10229..11374
                     /locus_tag="VEW08_05420"
     CDS             10229..11374
                     /locus_tag="VEW08_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ40208.1"
                     /translation="MIKRNRTLRQLGLVVSVISLLGLAGCGKGAAGDKPEKEATAKML
                     IAPEDVVTVSTNALASGPSITGSIEPERRADMRAEVSAVVLAVLKENGDPVKRGDLIV
                     RLDQTSIRDSLTAAEASARAADQAYEQAERQFQRMTTLQKTGVVSTQQVEDVEIRRNT
                     AQSDRASARTRMVTARQQLERTEVRAPFDGIVSDRKVSAGDTAAIGKELVKVIDPTSL
                     RFEGLVSADSIGQVKAGQHVWFKIHGFSTDEFTGVITRVNPAANVTTRQVEVLVAFDD
                     AKKQPNVAGLYAEGHVETQSSATLSLPSASIVKEGDSAFAWRVNGAKLQKVALTLGDR
                     DARTGAFALRGGLAEGDKVLRFPNSTLKDGQEVQTGAAPKPAIVAEK"
     gene            11387..14776
                     /locus_tag="VEW08_05425"
     CDS             11387..14776
                     /locus_tag="VEW08_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYJ40209.1"
                     /translation="MFLSNFSIRQPVTTVAIVIVLMCLGLLALKNLRVNQIPDVENPV
                     MAINFPYPGASPETVEREIINRVEKSLQSIPQVYEIWSNASESNANFTIIFDFKKDMS
                     EAGDEIRNAIASVRYKMPIEMREPVLFRRDPASEPIMNIALSSDKQTHAQISRLAEDK
                     LADEIRGVDGVSVVNIDGALHRELSVLLHAQKMREYNVSAGDVVNALRAQNTNAPVGR
                     IKGELGEQNIRLVGRIESPREFNDIVVKRQGGEIVRLGQVATVEDGFAEPGSYSLRNA
                     HPNVGMSVIRSREASTVTVAEKVREQIKEINKTLPEGTKLEVTYDGGEEASQNLNNVI
                     HALIFGAGLTIFVVYAFLNSWRSTLITGLSLPTSAVAAFIAIWLCGFTLNFMTLLGLS
                     LAIGVLIDDAIVVRENIVRHMERGEDRITAARNGTAEIGLAVTATTFSIVAVFVPVAF
                     MGGGAGEWFRPFAVTVVSSVMVSLFISFTLDPMLSAYWGDPPGHSTSERRGLGKVFAR
                     FNQWFDHQANGYGKVIEWALHHRVWMAVGAFALLILSLGYQGAFGRSEFLPKSDYGTL
                     AVEVRTPSSTSLEYARSKVEKAAELARELPEVESTDSRVNATGGRVWVNVGDKSDRSR
                     GLQELAADLRGRLKGLVGAEFVVLDDLNNGPRKPVQIQFYGTDSRKLMELTNQFMDKV
                     RQIPGAVDVGLSEQDPKDELRIELDRGLANQLGISVNDAAQTLRVAFAGVEVGDWVDP
                     TGESRDVAVRLSPEDRLDASNIEHLPISVAGTNMMVPLEQIATITIDKGPARIQHYNG
                     KRTVTVSANVQGVDAGTVTKEAKKIADAMEFPAGFGVGLGGASRDQNELFAEMGIALL
                     MGIFVMYLVLVMQFGSFTAPLAIMLSLLFSLIGVVLALNLTGGSLNLMSLIGVIMLMG
                     LVAKNAILLLDCAREEERQGIDREEALMHAGRVRLRPILMTTFALMAGMMPVAIGVGE
                     GGEFYSPMAVAIIGGTVVSTLLTLLVVPSFYDSIEIARERAAIKFHARSAYGNPLVAF
                     LSTLGEALITLLFIRFFYRSGRWLFDLDTPAEHPVERAARLVGHPVPAGFVARPKRWQ
                     VGRNSVVSTRTPPVSPDPQGIPTGPAPAPVPKPRWKPIKAS"
BASE COUNT         2590 a         4873 c         4821 g         2602 t
ORIGIN      
        1 tcagaagcag ctgtatcgcg ccgaacggcc caccacgacc tacgggtact acggcggcgc
       61 gccgggcaac atgatcctcg agggctttct gcagtgcggg tccggaccga tggatggcgc
      121 gcccacgcct gcgtcagcac ccgcgcccgc acccgcgcag gcgtcaggcc agtagctggt
      181 cggcgcggcc gcctatccag ctgcgcagga cggtgagctc ctcgtcgacg agcaccaggt
      241 ccgcgcgctg accgggcgcg atgcggccca tccggccgcc caacttcagg aactcggccg
      301 gatacgcgct cgccatgcgc agcgcatcgc tcagcggaac gccgagctgg ttcaccgtgt
      361 tgcggacggc gctggccatg tcgagatgcg cacccgccag ccgcccttcg tcgtcgacga
      421 tcttgtcgcc gtcgaccgtg atcagccggc cctggatccg gaactctttc acatccgcgc
      481 ccacactggg catggcgtcg ctcaccagca tgaagcggtc gtgccgtttg gcgcgcagcg
      541 caatcttcag cacttccgca tggacgtgat aaccatcgac gatcagtccc agccagctgt
      601 ccgggtgtgc cagcgccgcg cccacggcgc cgggttcccg gtttccgagt tgcgacatgg
      661 cgttgaacaa gtgcgtgaag ccgcgcacgc ctgcttcgag cgccggctgc agctcatcgt
      721 aagtggcgtt ggtgtgcccg gccgacacga tgactccggc gtccgtcagt gcgcggatga
      781 tcgcgggcgt cgtgcgttcg ggcgccagcg tgaccatggt gacgcctccg tgcggcgtcg
      841 ccagcagccg cattgcgtcc tcatcgagca cgcgcagctt gctggcatcg tgcacgccgc
      901 ggcgttcgtt gctgaggaat ggcccttcga tgtggatgcc gagcacgccg ggcacgccgc
      961 gcgcaatggc ctcgcgcacc gcgaggatgg cctgctccac gacgtgcaga tcgtcgctga
     1021 tcagcgtcgg cagaaatccc gtggtgccga accggcgatg cgcccggccg atcgtggcga
     1081 tggactcgac gctcggattg ccgttgaaca acacgccgcc gccgccgttc acctgcgtgt
     1141 cgatgaaccc cggcgccaga aggtgcccgt cgaggtcgat agtctgatcc gcgccgacga
     1201 cctcgcgtgc gccgctgatg gcttcgatat gcccgtcgcg caccagcagg caacgcccgc
     1261 tctcgatgcc ggcttcggtc aagatctttc cgttccggaa ggccagcagc atcagacggt
     1321 ctcggtgatc ttgttgagat gcggcggacg gtcgggatcg aaaccgcgcg ccagcgccag
     1381 cgtgtcgacc atggcgtaaa acgactgaat ggcgagcaac ggttcgatca ccgggttggc
     1441 ttcgatcgtg ggcagcgaca cggcaccgcg cgcctcggct ccggccagca acacgcgcgc
     1501 gccgcgcgcg gcgaactcgg cggcgagctc ctcgatgcca gcgcgagtgt cgtcacgctg
     1561 cgtgaacatg agcacgggaa atttcgcgcg caccagcgcc atgggtccgt gtttgacttc
     1621 ggcggagctg aaggcctcgg catgcagccc gcaggtttcc ttgagcttga gcgctgcttc
     1681 ctgcgcaacg cccaggccat aaccgcgggc gacgacgaac aggttcaccg cgccgcgcag
     1741 ggcatccacc gctggcgccc aatcgcactt ccacgcgcgc tgcagcagct cggggagctg
     1801 gggcaatgcc gccagcaact ttcgatcctc ggtccagtgg gcgagcagct gcgcgatgca
     1861 gctgagcgaa gcgatataag acttggtcgc cgccacgctg gcttcgggcc cggcgtgcag
     1921 cgggatcgcg acatccacga tacgcatgag cggggattcc ggtgaattgc acaacgcgac
     1981 ggtgagcgcg ccgccttcgc gcgcgttgcg cgtggcggtg agcaggtccg gactcatgcc
     2041 ggactgcgaa atggcgatga acaacacgcc gtcgagatcc gcctgcgatt cgtagatcga
     2101 gctcaccgaa ggcgcggccg acgaggtgag cacgtgcgtg tgcgactcga tgaggtactt
     2161 cgcgtaggtc gcggcgtgat cggagctgcc gcgcgcacag gtcaccaccg cgcgcggcgg
     2221 gttcttgcgc agccgggccc cgagtgccag cagcagcggc tcgttgtgcg tgagctgcgc
     2281 gcccaccact tgcggcgcag acgccgcctc ctggtacatc cgcgtctggg cgatcacgat
     2341 gatgcgctga gctccgccac gaagtcgtag atgtcgccac ggtagtaaga ctgcgaaaac
     2401 tccaccgcgc gcccgtcctt gagataaccg acacgcgcca ccagcaagcc cgcgtcgcgt
     2461 tcctgcgcct tgagcagctt ggcctgctcg gccgtcagca acacggcgcg cagccgctgc
     2521 agcgcacgca cggggcgatt gcctttctgt tccagggcct cgtacagcga ggattccacg
     2581 gcttcgagcg aaggcaggca gtcgcgcaac accgtcgcgt attccagcgc catgggtgca
     2641 tcgtcggcat aacgaatgcg actgaagcga tagaccgccg tgccgggact cgagcgcagc
     2701 gtcagggact cttcgggtgt gaccgtgccc tcggcacgac tcagccacac gctgcgcggc
     2761 tcgcgcccgc gtgcccgcat gtcttcggag aacgaagtca gcttcgcgaa attcttctcg
     2821 acgcgattgc tgacgaacgt gcccgaacct tgcttgcgcg tgagcagccc ttcctcgacg
     2881 agttcttcga tggctttgcg cacggtgatg cgcgacaccg aaagcatgtc ggcgagatcg
     2941 cgctccggcg gcaaagcatc gtcgggcccg atgaccccgt tctcgatcgc ttcgcgcagc
     3001 gcgcgttgca gttgctgata gagcggctgt gagctcgatt catcgagctt cggtaatacc
     3061 tgagaaagtg taggcaagac ttttaccccc tgatcctggt ggaaccatgc cattcagata
     3121 gaaccaatgc aataccaata tgaacctctg tgacgcatga gacccatata acgcactgtt
     3181 tttcagaaaa aaacctgctg atccgggcat tgcagacaag aaactggttt ctctgagacg
     3241 attggtacta tattggaccc atattggtct gtatgaaaac cgaactgcca aaccgattca
     3301 tgagcaccac aagtctgacc ttgagggcca atgccacgcc gccggccacc gcgaaacacg
     3361 tggcaagaaa atcgggcgcg gaaattgaaa ccgtcgtagt ccccggtacc caccccagtc
     3421 tcgagtccca gaccgttatt gatgttctca ctgcgtacaa gaacgtggcc gaagccttgc
     3481 agcgtgttag tggcgcgagc atctcacgcg agttcggcgc gggcgagcgc gcttcgatct
     3541 gcggtacggc gccgcgcatc tcgggttgct tgagcgcgtt tatcaggcag gattcagttt
     3601 ctttgatttg actcggattc cggggacagg caggcaatga acaagaacca ggtttcgtat
     3661 ttggcgctca gcgtcgtgtt cggattggcg gcgggctgcg gctcgcccga cgcaacaccg
     3721 gcgaagcctg tacagctcga cgcgccgcat ctggggctga tacccacgcc cgcccacagc
     3781 aacggggcag ttggaacctt ccgcgtcgcg gcgaccaccg atgtcctttt ctcgggtggc
     3841 gacggtgcgt ctgatgtcgc ccagtacttc gtcgagctca tcaaggagca gcggccggat
     3901 ctggcgtttc gcaagcctcg agaggacacg ccttcgtccg gcagcatcgc cttcgtgctg
     3961 gccgccgacg agaaaagtca gggtgcggaa ggttattcgc tcgcggtgac tcccaagggc
     4021 gtcaccatca cggcgcgcac gccggcgggc ctcttttatg gggcagtcac gctgtggcag
     4081 ctcatcacgg cggaagccat gcagggcctg tccgttgaca tacccgcgct ccggatcacc
     4141 gacgcgccac gcttcgcgtg gcgcggagtc atggtcgatt ccgcgcggca cttccagtcg
     4201 cccgaataca tcaagcagtt catcgactgg atggcactgc acaagctcaa cgtcctgcac
     4261 tggcatctgg tcgacgacca gggctggcgt ctcgagatca aaaagtatcc ccgccttact
     4321 tctattggcg catggcgcgt gccggctgga caggctccgc tcaccgacat cgacacggcc
     4381 accggcaagc cgcggcaata cggcggcttc tacacgcagg accaggtgcg cgacatcgtg
     4441 gcgcacgccg cgaagcggca catcaccgtc gtacccgaga tcgagatgcc gggacacgcg
     4501 agtgctgccg tggtcgcgta tcccgagctc ggggtcacta acaagcctcc gaaggccgtg
     4561 cccgaggagt ggggcatttt cccgacgctg ttcaacatcg acgactccac gttcacgttc
     4621 ctggaaaacg tgctcgccga aaccatcgag ctctttccga gtgagtacat ccacgtcggt
     4681 ggcgatgagg cgctgaaaga cgagtggcac gcttcaccga agatccaggc gcgcatgaaa
     4741 gagcttggga tcaaggatga acatgcgctg cagagctact tcatccagcg catcggcaag
     4801 tacatcgact cgaaggggcg caagctcatc ggctgggatg aaattctcga aggcggtctc
     4861 gcacccaacg ccaccgtcat gtcgtggcgt ggcatcgaag gcgccatcgc cgccgcgaag
     4921 gcgggccatg acaccgtact gtcgcccgcg cccgatctgt atttcgatca ctggcaaagt
     4981 cccggtgaca cttctcccgg ccgcagcaac acgctgtcgc tcaaggacgt atacctgttc
     5041 aatcctctgc ccgcggccat ccagcccgag cagcgcaaac acgtgttggg attgcaggcc
     5101 aacctctggg cggagttcat gcgctccgaa gcgcgcgtga cctaccaggc ctatccgcgc
     5161 gtggcggcgc ttgccgaagt ggcatggtcg cctccggagc aaatcaactg ggacaacttc
     5221 cagaagcgcc tcgaaccgca acttcgccgc tacgccacgc tcggcatcgg ctacgcgcgc
     5281 gaaacgccgg tggtgcccgg accgcgccga cgcgtgagcc acgatctcga gcagtgtggc
     5341 ggtggttacc tgctgtcgct cgaagacgat gcaccgctcc agggggagcg cgccgtgttc
     5401 ctcgtgaaca tcaccgatcc ctgctggatc tggaagggcg tggatctgtc gcaggtgaag
     5461 gcgattcgcg ccacggtagg ccagattcct ttcaacttcc agatcggcgc ggatgccaaa
     5521 aagattccgc tgcacaagcc cgcgacgaaa tccggcgagc tggaaatccg actcgatgac
     5581 tgcaagggca aagtggtggc gagcgcttcg ctcgaaccgg ccgtgtccaa tgtcggcctc
     5641 acaccgttgc cgcccatcga aatgccctcg caggaggggc agcacgacat ctgcttcacg
     5701 ttcacgcgag ccaaggtcga tcccatctgg gccatcggca gtctcgagct cattggttat
     5761 tgacgatgcc gctgatcatc cgcgccccat ttgccggttg gtgcgcgccg ctttcggaaa
     5821 ttcccgatgc cgccttcgcg caaggcttgc tgggcgatgg cgtggggctc gatcccaccg
     5881 gcaatgagct gcgttcgccc tgcgacggag aggtcatctc ggtcgccgct tcgcgccacg
     5941 cgctcgcact gcggaccgcc gccggcgcgg aaattctcgt gcacgtgggg gtcgacaccg
     6001 tgggactggc gggcgaggga ttcaacgtgc acgtgcgcaa gggtgaccgc gtgcatgccg
     6061 gcgacctgtt gatcaccttc gatctcgatc tgctggcacg caaggcgccc agtctcatga
     6121 caccggtcat cgtcaccaat gcggaccgct tccgcatcgc gcaggcgaat acgcggcgga
     6181 aggttgccag cggcgatgcg ctgttcgaaa tcgaacagtt cgcggccggg gaatcgggcg
     6241 acacggggga gcccgcggtg tccggcgcag tcgtggtcag cgaatccgtc gttgtggatc
     6301 acgcccacgg catccacgcg cggccggcag cgcagatcgc gcgcatcgcc aaggggctgc
     6361 cctttgaaat cgaattgcgc gcccgcggtc gcggcgcgcg cgcccgcagc accgtggcgc
     6421 tgatgtccct cgggatccgg ggcggtgacg aagtcgtcat cgtcggcttc gagccggcgg
     6481 ccgccgtcgg aatcgcggcg atcgtcaagg cgattcgcca gctcgaacgc gtcgcgccgt
     6541 ctgcggcggt cgcaccggtg gcggccgcgc cggtcggcga tacctcgcaa ctgcgcggcg
     6601 tcatcgccag cccggggctt ggcgtaggca cggcgttcta tctacacgca gcggaacttc
     6661 cggtcactga agccggccgc ggcgtgcagc aggaaagcac cgatttcgaa cgtgcccgcg
     6721 acgcggttcg cgcgcgcttg tcacggctcg cgaccaccgc tgcgccgacg ttgcgcgaaa
     6781 tcatgtccgc gcacctcgaa ctgctcgatg accccgatct cgtcgatggc gcgcgcgcag
     6841 ccatcgccgg cggcaagagc gcgggattcg cctggcggga gagtctgcac gtcagcgcgc
     6901 gggccctgag cgccacgggc gatgcgcgcc tggccgaaag agtcgacgac ctgcgcgacc
     6961 tggaaaggca gttgttgctc gcgctcgatg gagcgcaggc cccgagcttc gtgattccgc
     7021 ccgagggcat cctgctcgcg cgcgatctga cgccctcgca gctcgtcgat atcgatgcat
     7081 cgaagctcgc gggcatcgcg ctggccgcgg gcggtcccac ctcgcacgtt gcgattctcg
     7141 ccgcgactct cggcgttccc atgttggtgg ccatgggccc atccctgctg gaggtctcgt
     7201 cgggcagcaa ggtcatcctc gatgccgatg cgggcatcat gcgcgccgat ccggatgcgc
     7261 agacgctggc ggaaacacgg cgacggctcg aacgcggccg cgcgttgcgc gagcagcaga
     7321 ccgccgccgc gcaggtggat tgccatctcg cgagtggtga gcgcatcgaa gtgttcgcca
     7381 atctcaccgg ctcggaagcg gatgccacgc ttgccgcggc ccagggcgcc gaaggctgcg
     7441 gcctcctgcg cacggaattc ctgttcctcg agcgcgagac cgcgcctgac gaagaggagc
     7501 aactgcgctg ctacagccag gtggcgcgga ttctcggaac ccggccattg atcatccgca
     7561 cgctcgacat cggcggcgac aagcccattc catatctgcc gctgccgccg gaagacaacc
     7621 cggcgctggg attgcgcggt gtgcgcacca gcctgtggcg tccggatctt ctcgaggtgc
     7681 agctgcgcgc gatgctgcgc gtgcagcccg cgggagtggt gcgcatcctg ctgccgatga
     7741 tcaccgacgt cgcggagatt cgcgccgtgc ggcggttgtt ggacgaggcc tgcgccaggc
     7801 tcgaggtgtc gccgcctgtg ctcggcgcca tgatcgaaac gccggccgcg gccgtgctcg
     7861 ccgatgccat tgcgcgcgaa gtcgactttc tgtcgatcgg caccaacgat ctcgcgcagt
     7921 acacgctggc gatggatcgc ggtcacgccg agctggccgc gcgcatcgac ggggttcatc
     7981 cggcggtcct gcgcatgatc gacatcacct gcaggggagc ggccgcgtcc ggcaagccgg
     8041 tggccgtgtg cggcgggctc gccgcggatc cgtcggcggt tccggtcttg ctgggccttg
     8101 gcgtgagcga attgtcagtg gtgcccacgc tgattccgca gctcaaggcg caagttcgcg
     8161 cgctgacgct cgaagcctgc cgggcgaccg cggcccgcgc actggcgctg gaaagcgctg
     8221 cgcaggtgcg tgcgttatga acaagatcat cggaaagctg caacccatcg gccgcgcgct
     8281 catgttgccg atcgcggtgt tgccggtggc ggcgctgctg cttcggctgg ggcagcctga
     8341 cctgctcgac gtgccggcga tcgccgccgc gggcgacgcc atcttctcga acctggggtt
     8401 gctgtttgcc atcggtgtcg ccgtgggtct cgcacgggag aatcacggcg cggccggact
     8461 cgccgcagtg gtgggttacc tggtggccac gcagggcgcc aaggtgctca tcgatgtgcc
     8521 gccggaagtc accgccggca ttcccgatca gctgaaagac atggcggcag cggccttccg
     8581 cgcgaaggag ctgtccaagc tcagcgtgcc cgtgggcctg ttgtccggac tcatcgcagg
     8641 ctgggcctac aaccgcttca gcgacatccg cctgcctagc tacctggcgt tcttcggcgg
     8701 ccgccgcttc gtgcccatcg tcagcggatt cgcggggctc gggctggcgc tggctttcgg
     8761 tttcggctgg cagtggctcg agagcggcat ggacgcaacc agccaggcgg tgctcggttc
     8821 cgaatccgtc gggctcttca cctacggcgt attgaaccgc gtgctcatcg tcaccgggct
     8881 gcaccacatc atcaacaaca tcgcgtggtt cctgttaggc gactaccagg gcgtcaccgg
     8941 tgacctgaag cgtttcttcg ccggcgaccc gtcggccggc gcattcatgt cgggattctt
     9001 cccggtgatg atgttcgggc tgccgggcgc ctgcctcgcc atgtatcgca ccgcgactcc
     9061 cgcgcggcgc gccgccgccg gcggcctgct gctctcgatg gcgctcacgt cgttcctgac
     9121 cggcgtcacc gagcccatcg agttcgcgtt catgttcctg gcgccggtgc tgtacgtcat
     9181 tcacgcggtg ctcaccggca tcgccatggt tctgatgcac gcgctcgatg tgcacctggg
     9241 tttcggtttc tcggctgggc tattcgacta cgtgctcaac tattcgctgg cgacccgccc
     9301 gctgatgctc attcccgtcg gtctgggcta tttcgcgctg tattacttcc tgttccgtta
     9361 ctgcatcgag cgtttcaatc tgaagacgct gggccgcgaa gccgatggtg ctgcggtagc
     9421 caccgttgcc gccggatcgg agaccggacc cgccgccggc tggattcgcg gcctcggaca
     9481 ggcggccaat ctgcgcagcg tcgaggcttg cacgacgcgc ctgcgactgg tgttgaacga
     9541 tccgagcctg atcgacgagg ccgcgctgac ggcgctgggc tcccgcggcg tgctgcgaat
     9601 cggtgacggc gccgtgcagg tggtcgtggg ccccatcgcc gatcagctcg cctccgaaat
     9661 ccgcgcgcaa tggcgcgcgc cgacgcctgc ggtgtccgca cccgcaccag tgccatctcc
     9721 cgcgccggca ctcgcggcgg cgccggatcg atcgatactg gcgggagtcc tcgccgcgct
     9781 cggcggccgc ggcaacatca ccggtatgca tgccaattcg acgcgcctgg tcatctcggt
     9841 gcgtgatccg gcagccgtgg acgaatccgc gctcgggcgc ctggccaatg ccatcgcccg
     9901 gccggcgccg gccagcctgc atctcatcgt cgggccggaa gcgcgaagct ggctcgcggt
     9961 catggaaacc ctgacgagct gaaaaagggg tccgggcgag cctcggatgc gccgagccga
    10021 ccggaaatgc cggttcgccg tgacaactca cactttcaaa cgaatcagcg gcttactaaa
    10081 ctgccgccct tctgagaagg gaattgcggg ccgatccggc ggctgacgca tgaattccca
    10141 accgaaacaa cacatttatg tctggggcgg agtgcaaccc aaaaggtcgc tcacacgtcg
    10201 tatgtcgggg cccccgaagg aatatcgcgt gatcaaaaga aacagaactc tccgccagct
    10261 gggactggtg gtctccgtca tttcgctgct cggcctcgcc ggttgcggca aaggtgctgc
    10321 cggcgacaag ccggaaaaag aagcgaccgc caaaatgctg atcgcacccg aggacgtggt
    10381 cacggtcagc accaatgcgc tggcgtccgg accttccatc accggatcca tcgagcccga
    10441 acggcgcgcg gacatgcgcg cggaagtttc ggccgtggtt ctggcggtgc tcaaggaaaa
    10501 cggcgacccg gtgaagcggg gcgatctcat cgtgcgactc gaccagacgt cgatccgcga
    10561 cagcctgacc gcagccgaag cttcggcgcg cgccgccgac caggcgtatg aacaggccga
    10621 aaggcagttc cagcgcatga ccacgctgca aaagactggc gtggtgtcca cccagcaggt
    10681 cgaagacgtg gagatccgcc gcaataccgc gcagagcgat cgggcctcgg cccgcacgcg
    10741 catggtcacc gcccgccagc agctcgaacg caccgaagtg cgcgcgcctt tcgacggcat
    10801 cgtcagcgac cgcaaggtgt cggcgggcga cacggccgcc atcggtaagg aactggtcaa
    10861 ggtcatcgat ccgaccagcc tgcgtttcga aggactggtg tcggccgaca gcatcggcca
    10921 ggtgaaagcc ggtcagcacg tgtggttcaa gatccacggc ttttcgacgg atgaattcac
    10981 gggcgtgatc acgcgcgtca atccggccgc caacgtgacc actcgccagg tcgaagttct
    11041 cgtggccttc gacgatgcga agaaacagcc gaacgtggcc ggtctctacg ccgagggaca
    11101 cgtcgaaact caaagcagcg cgacactgag tcttcccagc gcatccatcg tcaaggaagg
    11161 cgacagcgcg tttgcgtggc gcgtcaacgg tgcgaaactg cagaaggtgg cgctgaccct
    11221 gggtgatcgt gacgcgcgca ccggtgcatt cgcgctgcgc ggcgggctcg ccgagggcga
    11281 caaggtgctt cgcttcccga actccacgct gaaagacgga caggaagtgc agacgggcgc
    11341 cgcgccaaag cccgcaatcg tcgccgagaa atagcggagg ctcccgatgt tcctttccaa
    11401 cttcagcatc aggcagccgg tcaccacggt tgccatcgtg atcgtgctca tgtgcctggg
    11461 gctgctggcg ctgaaaaatc tgcgcgtcaa ccagattccc gacgtcgaaa acccggtgat
    11521 ggcgatcaac ttcccgtatc cgggcgcatc gccggagacg gtggaacgcg aaatcatcaa
    11581 tcgcgtcgag aaatcgctgc agagcattcc gcaggtctac gagatctggt cgaacgcgtc
    11641 cgagagcaac gccaacttca ccatcatctt cgacttcaag aaggacatgt ccgaggcggg
    11701 tgacgagatc cgcaacgcca ttgcgtccgt gcgctacaag atgccgatcg agatgcgcga
    11761 gccggtgttg ttccggcgcg atccagcctc agaaccgatc atgaatatcg cgctgtcgtc
    11821 ggacaagcag acccacgcgc agatctcgcg gttggccgag gacaagctgg ccgacgagat
    11881 tcgcggcgtt gacggcgttt cggtggtcaa catcgacggc gcgctgcatc gcgagctctc
    11941 cgtgctgctg cacgcgcaga agatgcgtga gtacaacgtt tcggcgggcg atgtcgtgaa
    12001 cgcactgcgc gcgcagaaca ccaacgcccc ggtgggccgc atcaagggcg aactcggaga
    12061 acagaatatc cggctcgtcg gccgcatcga atcgccgcgg gagttcaacg atatcgtcgt
    12121 gaagcgccaa ggcggcgaaa tcgttcgcct gggccaggtc gcgaccgtgg aagacggctt
    12181 cgccgaaccc ggcagctact cgctgcgcaa cgcccatccc aatgtcggca tgtccgtgat
    12241 ccgttcgcgc gaggcgagca cggtgacggt ggcggaaaag gtgcgcgagc agatcaagga
    12301 aatcaacaag acgctgcccg aggggaccaa gctcgaggtc acctatgacg gtggcgaaga
    12361 agcgtcgcag aacctcaaca acgtgattca tgcgctcatc ttcggcgccg gcctcaccat
    12421 tttcgtcgtc tacgcgttcc tgaattcctg gcgctcgacg ctgatcacgg gtctgtcact
    12481 gcccacctcg gccgtcgccg cgttcatcgc catctggctg tgcggcttca cgctgaactt
    12541 catgacattg ttaggcctgt cgctggccat cggcgtgctc atcgacgacg ccatcgtggt
    12601 gcgcgaaaac atagttcggc acatggaacg cggggaagac cgcatcacgg ccgcgcgcaa
    12661 cggaaccgcg gaaatcggtc tcgcggtcac ggccaccacg ttctccatcg tcgcagtgtt
    12721 cgtgcccgtg gcgttcatgg gcggcggcgc gggtgagtgg ttccggcctt tcgccgtgac
    12781 cgtggtgtca tcggtgatgg tcagcctgtt catttcgttc acgctcgacc cgatgctgtc
    12841 ggcctactgg ggcgacccgc cgggacattc gacgagcgaa cggcgtggcc tcggcaaggt
    12901 gttcgcgcgc ttcaaccagt ggttcgacca ccaggccaat gggtatggaa aagtcatcga
    12961 gtgggcattg catcaccgcg tctggatggc ggtaggcgcg tttgcgttgc tgatcctctc
    13021 gctgggatac caaggtgctt tcggtcgcag cgaattcctt cccaagtccg actacggcac
    13081 gctggccgtc gaagtgcgca cgccgtcgtc caccagcctc gaatacgcgc gcagcaaggt
    13141 ggaaaaggcc gcggagctcg cgcgcgaact tcccgaagtg gagtccaccg acagccgcgt
    13201 caacgcgacc ggtggccgcg tgtgggtcaa tgtcggcgac aagtctgacc gttcacgtgg
    13261 gctccaggag ctggcggccg atttgcgcgg cagactcaag gggctggtcg gcgcggagtt
    13321 cgtggtgctc gacgacctca ataacggacc tcgcaagccg gtgcagatcc agttctacgg
    13381 gacggactct cgtaaactca tggagctcac gaatcagttc atggacaagg tgcggcagat
    13441 cccgggcgcc gttgacgtcg gcctctcgga acaggatccc aaagacgagc tgcgcatcga
    13501 actcgatcgc ggactcgcga accagctcgg catttccgtg aacgatgccg cgcaaacgct
    13561 gcgcgtggcg ttcgcgggcg tcgaggtggg cgactgggtg gatccgacgg gtgaatcgcg
    13621 cgacgtcgca gtccggctgt cacccgaaga tcgcctcgat gcgtccaaca tcgaacatct
    13681 gccgatctcc gtggccggca ccaacatgat ggtgccgctg gaacagatcg ccaccatcac
    13741 catcgacaag ggtccggcgc gcatccagca ctacaacggg aagcgcaccg tgacggtttc
    13801 cgcgaacgtg cagggcgtcg acgccggcac cgtgaccaag gaagcgaaga agattgcaga
    13861 tgccatggag ttccccgccg gtttcggtgt cggacttggc ggcgcttccc gcgatcagaa
    13921 cgagctgttc gccgagatgg gcatcgcact gctcatgggc atcttcgtga tgtacctggt
    13981 gctggtgatg cagttcggtt cgttcaccgc gccgctcgcc atcatgttgt cgttgctgtt
    14041 ctcgctgatc ggcgtcgtgc tcgcgctcaa tctcacgggc ggctcgttga acctcatgag
    14101 cctcatcggt gtcatcatgc tcatgggact ggtggccaag aacgccatcc ttttactcga
    14161 ctgcgcgcgt gaggaagaaa ggcagggcat cgatcgcgaa gaagcgctca tgcacgcggg
    14221 ccgggtacgc ctgcgcccga ttctcatgac cacgtttgcg ctgatggccg gcatgatgcc
    14281 ggtggccatc ggcgtcggcg aaggcggcga gttctattcg ccgatggcgg tggccatcat
    14341 cggtggcacg gtcgtatcga cgttgctcac cttgctggtc gtgccgagct tctacgacag
    14401 catcgagatc gcgcgcgagc gcgccgccat caagttccat gcacggtcgg cgtacggcaa
    14461 tccgctcgtg gcgttcctga gcacgctggg cgaagccctg atcaccctct tgttcatccg
    14521 cttcttctat cgatcgggcc gctggctgtt cgacctcgac acgccggccg agcatcccgt
    14581 ggagcgtgct gcgcgcctgg tcggccaccc ggttccggcc ggtttcgtgg cgcggcccaa
    14641 gcgttggcag gtcggacgca actcggtggt cagcacgcgg acgccgccgg tatcccccga
    14701 tccgcagggg ataccgactg ggccggcgcc ggcgcccgtg ccgaagccgc gctggaagcc
    14761 gatcaaggcg agctgatcag cggatcagtt cgcgacctgc agccattgct gcgtggtcat
    14821 gccgatcctg cccgcgcccg cgccctgcgc accggtgagc agggcgggca acgcgctaac
    14881 cggatc
//
LOCUS       DASQJS010000130         4549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3214567, whole genome shotgun sequence.
ACCESSION   DASQJS010000130 DASQJS010000000
VERSION     DASQJS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3214567"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VEW08_05430"
     CDS             <1..715
                     /locus_tag="VEW08_05430"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40210.1"
                     /translation="TYTARLRVMDSAGQMAETTRSVSIVAVGEVSSQVVVNYSGPGDG
                     LIEIRSTEPGFVPVRCSTSTPAGQPTDTGTCTATVRAGYDITVAAEYDPVRSTFSGWS
                     CRDVVTSTTPSGTRTECRFIANNRDLTFEATFLIAPPLTEILRVELHPFSQGGGEIFD
                     ASGRVYCTVQNQRIRTLQDGICSYSFDWGTTIHLYAAAQPFGSRFVGWQGCDSQPSPT
                     ECVVSMTRLREVTAHFSGG"
     gene            723..1619
                     /locus_tag="VEW08_05435"
     CDS             723..1619
                     /locus_tag="VEW08_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ40211.1"
                     /translation="MLELEPQLWHFHDAPSGTFTHLLACPITRRAALIDPVLGFEPGT
                     AHIQTAAAQRVAAVIRAEHLVLDWVLETHIHADHVSAAQFFKQRFGARVGIGYRVREV
                     QRLYAPLFWGARDLATDGSQFDRLWQDDDRIIVGGIEIHVLATPGHTLDGVTYRAGRH
                     AFVGDTVFRADFGTARTDLIGGDAATLYQSISRLYALQPGTILHFCHDYPRGGAPPTE
                     QMEVDRMRTENAQLSLATSEDEFVTLRQLRDATLPVPLLLIPAVQINIAAGQLPCAEA
                     NGISYLRVPLNSFGGVTVAMGE"
     gene            1713..3170
                     /locus_tag="VEW08_05440"
     CDS             1713..3170
                     /locus_tag="VEW08_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019096710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HYJ40212.1"
                     /translation="MPAVSRAVPVGEFYELEVGADLAASSRFNEDIAPTRVAQRTWNE
                     WHIAALWVGMAICVPTYTLGGVLTTYFGLSVGEALWAVFIANVIVLIPLTLNAFGGTK
                     FGIPFPVMLRSSFGIIGSNVPALIRALVACGWFGIQTLFGGIAVHLMLAALSESWAQL
                     GGVGEVIGFFIFWVANVWVVLRGSESIKILEVIAAPLLMLVGVGLLVWAWPQASVSEL
                     LARPPNRPANASFWGYFFAGLTAMVGFWATLSLNIPDFSRFARSQRAQIVGQIVGLPV
                     TMVLFAGLGVVMTAASAQLVGETVSDPITLIGKIDSPFWVVLSMMIIIIATISTNTAA
                     NIVSPTNDFQNLAPRLINQKKGVLLTGFIGIALMSWELLKRIGWLESDVSVDSLYSNW
                     LLGYSSLLGPISGIMVVDYFVVRRQLYDLPALYLNQGAYPAWNVAGFVALGIPVALTI
                     IAITTGALQWFYTYGWFTGSLLGGLIYYLMSRARS"
     gene            3192..>4549
                     /gene="hydA"
                     /locus_tag="VEW08_05445"
     CDS             3192..>4549
                     /gene="hydA"
                     /locus_tag="VEW08_05445"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020300566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HYJ40213.1"
                     /translation="MTVLVKGGTIVTADQTYRGDVLCADGKIVSIGEKVAAPVNAEVV
                     DAGGQYVMPGGIDPHTHMQLPFMGTVASDDFYSGTAAGLAGGTTMIIDFVIPNPKQRI
                     LHAHRQWREWAQKSVADYSFHVAITWWDETVREDMGTLVREFGVNSFKHFMAYKNAIM
                     CDDETLVNSFSTALSLGAIVTAHAENGDLVFKLQDEIYKRGITGPEGHPLSRPPEVEG
                     EAANRAIRIAEVLKVPIYLVHVSSRQGLEAITRARNEGQRVFGEVLAGHLLLDDSVYR
                     DPDFTRAAAHVMSPPFRAPEHQVALWRGLQSGNLQTTATDHCTFCADQKAAGKDDFRK
                     IPNGTAGIENRMEVLWHHGVNTGRLTMNEFVRVTSTNAAQIFNIYPRKGSVSVGADAD
                     LVVWDPAASKTISAKTHHQKVDFNIFEGMQVKGGASHTVSGGKLVYARGDLRVERGAG
                     NH"
BASE COUNT          816 a         1410 c         1446 g          877 t
ORIGIN      
        1 cacgtacacg gcgcgcctgc gagtcatgga ctcggccggg caaatggccg agaccacgcg
       61 tagcgtgagc atcgtggcag tcggtgaagt ctcgagccag gtcgtcgtga actactcggg
      121 gcccggagat ggactcatcg agattcgcag cacagagccg ggtttcgtac cggttcgatg
      181 ttcgacgagc acgccggctg ggcagccgac cgacaccggg acctgtaccg cgacggttcg
      241 cgcgggatac gacatcaccg tcgccgccga gtacgatccg gtgcgctcga ctttctcggg
      301 ctggtcgtgt cgcgacgtcg tgacgtcgac aacgccgtcg ggcacgcgaa ccgagtgccg
      361 cttcatcgcc aacaatcgcg atctgacgtt cgaagccaca ttcctgatcg cgccgccact
      421 caccgagata ctgcgcgtcg agctgcaccc tttcagtcag ggaggcgggg aaatcttcga
      481 cgccagtggc agggtctact gcaccgtgca aaatcagcgg atccgcacgc tgcaggatgg
      541 catctgcagc tacagcttcg actggggaac gacgattcat ctctacgccg cggcgcaacc
      601 gttcggcagc cgtttcgtgg gatggcaggg atgcgattcg cagccgagcc cgaccgagtg
      661 tgtggtctcg atgacgcgtt tgcgcgaggt cacggcacat ttttccggag gataggcgac
      721 gcatgctcga gctcgagccc cagctgtggc attttcacga tgcaccgagc ggcacgttca
      781 cgcacctgct ggcatgcccc atcacgcgtc gcgcggcgct catcgatccg gtgctgggtt
      841 tcgagcccgg caccgctcac atccagactg ccgctgcgca acgcgtcgcg gcggttatcc
      901 gcgcagaaca cctggtgctc gactgggtgc tcgaaactca catccacgcg gatcacgtct
      961 cggctgcgca gttcttcaag cagcgattcg gtgcgcgcgt gggcatcggt tatcgcgttc
     1021 gagaagtgca gcggttgtac gcaccgctgt tctggggtgc tcgtgacctt gcgaccgacg
     1081 gctcgcagtt cgatcgactg tggcaggacg acgatcgcat catcgtcggc ggtattgaaa
     1141 tccacgtgtt ggcgactccc ggccacacgc tcgacggagt gacctatcgc gccggccgtc
     1201 acgcgttcgt cggcgacaca gtcttccgcg cggacttcgg aacggcgcgt accgacctca
     1261 ttggcggcga cgcggcaacg ctataccaat ccatttcgcg gctgtatgcg ttgcaacccg
     1321 ggaccatcct tcatttctgc catgactatc cgcgtggcgg tgcgccgccc accgagcaga
     1381 tggaggtcga caggatgcgc accgaaaatg cccaactgtc gctggccacg agcgaggatg
     1441 aattcgtcac tctacgccaa ttgcgggatg cgactcttcc cgtgcctctg ctgctcattc
     1501 cggcggtgca gatcaatatc gcggcgggac agcttccttg cgccgaggcc aacggcatct
     1561 cctatctaag agtgccgctc aacagcttcg ggggcgtaac tgtcgcaatg ggcgagtgac
     1621 tttctggttc agcaagctat attggcgtgg ctcgcgcccg ggagttgaaa aggagcaacc
     1681 ttgctgtgcc tcgagaagat cttcacacgg ccttgcctgc ggtaagccgt gcggtaccgg
     1741 tcggcgagtt ctatgagctc gaggtaggcg cggatctcgc cgcgagctcg cgcttcaacg
     1801 aagacatcgc gccgacacga gtcgcgcaac gcacctggaa cgagtggcac atcgccgcgt
     1861 tgtgggtggg catggcgatc tgcgtgccga cctacacgct cggtggcgta ctgacgacct
     1921 acttcgggct ctcggtcggc gaggcgctgt gggcggtgtt catcgccaac gtcatcgtgc
     1981 tcataccgct gacgctcaac gccttcggcg gcacgaagtt cggcattcct ttcccggtga
     2041 tgctgcgctc gtcgtttggc atcatcggtt ccaatgtgcc cgcgctgatc cgcgcgctgg
     2101 tggcgtgcgg ctggttcgga atccagacgt tgtttggcgg catcgccgtg cacctgatgc
     2161 tggcggcatt gtcggaaagc tgggcgcaac tcggcggcgt gggcgaagtc atcggcttct
     2221 tcatcttctg ggtcgccaat gtctgggtgg tattgcgcgg ctcggagtcc atcaagatcc
     2281 tggaggtcat cgcagcgcca ctgctgatgc tcgtgggcgt ggggctgctg gtctgggcct
     2341 ggccgcaggc ttcggtcagt gagttgttag cgcggccacc caaccggccg gccaacgcct
     2401 ctttctgggg ctacttcttc gcaggcctca ccgcgatggt tggcttctgg gcaacgctgt
     2461 cgctgaacat tccggatttc agtcgattcg ccaggtcgca gcgcgcacag atcgtcgggc
     2521 agatcgtggg attacccgtc acgatggtgc tgttcgcggg tctgggcgtg gtgatgaccg
     2581 cggcgtccgc gcagctcgtc ggtgaaaccg tgtccgatcc gatcacgctg atcggcaaga
     2641 tcgacagccc gttttgggtc gtgctgtcga tgatgatcat catcatcgcc accatctcga
     2701 ccaacaccgc ggcgaacatc gtctcgccca ccaacgattt tcagaacctg gcgccgcggc
     2761 tcatcaacca gaaaaaaggg gtgctgctca ccggcttcat cggcattgcg ctgatgagct
     2821 gggaactgct caagcgcatc ggctggctcg aatcggatgt cagcgtcgac agcctgtatt
     2881 cgaactggct cctcggctac tcgagcctgc tcggccccat ctcgggcatc atggtggtgg
     2941 actactttgt ggtgcgccgg caactctacg acctgccggc cctgtacctg aaccaggggg
     3001 cgtatccggc ctggaatgtc gcgggtttcg tggctttggg gataccggtc gcgttgacga
     3061 taatcgcgat caccaccggc gctctgcagt ggttctacac ttacggctgg ttcacgggat
     3121 cgctcttggg cggactcatc tactatttga tgagccgcgc caggagttga gcaaggagct
     3181 gaggtagcgc aatgacagtt ctagtcaagg gcggcaccat cgtcacggcc gatcagacgt
     3241 atcgcggaga cgtcctgtgc gcggacggaa agatcgtgag catcggtgag aaggtcgcgg
     3301 cacccgtaaa cgccgaagtg gtcgacgccg gcggccagta tgtgatgccg ggcggcatcg
     3361 atccgcacac gcacatgcag ctgcccttca tgggcacggt ggccagcgac gatttctatt
     3421 ccggtaccgc ggcgggcctt gccggcggaa ccacgatgat catcgacttc gtcattccca
     3481 atccgaaaca gcggatcctc cacgcacacc ggcagtggcg cgagtgggcg cagaaatccg
     3541 tcgcggacta ctccttccac gtggccatca cctggtggga cgagaccgtg cgcgaagaca
     3601 tgggcacgct ggtgcgcgag ttcggcgtca acagcttcaa gcatttcatg gcgtacaaga
     3661 acgccatcat gtgcgacgac gagacgctgg tgaacagctt ttccacggcg ctgtcgctgg
     3721 gcgccatcgt gacggcgcac gccgagaacg gcgatctcgt gttcaagctg caggatgaga
     3781 tttataaacg cggcatcacc gggcccgaag gccatccgct gtcgcgcccg ccggaagtcg
     3841 agggtgaagc cgcgaaccgc gccatccgca tcgccgaagt gctgaaggtc cctatctacc
     3901 tggtgcatgt ttcctctcgc cagggactcg aggccatcac gcgggcgcgc aacgaaggtc
     3961 agcgcgtgtt cggcgaggtg ctcgccgggc acctgctgct ggacgattcg gtgtaccgcg
     4021 atccggattt cacgcgtgcc gcggcgcacg tgatgagccc gccgttccgc gcacccgaac
     4081 accaggtggc gctgtggcgc ggattgcaat ccggcaatct tcagaccaca gccaccgatc
     4141 actgcacgtt ctgcgcggat cagaaagccg cgggcaagga cgacttccgc aagattccga
     4201 atggcaccgc cggcatcgag aatcgcatgg aagtgctgtg gcatcacggc gtgaacaccg
     4261 gacgcctgac gatgaacgag ttcgtgcgtg tcacgtccac caacgccgcg cagatcttca
     4321 acatctatcc gcgcaaaggc agcgtctcgg tgggcgccga tgcggacctc gtggtgtggg
     4381 atcccgcggc aagcaagacc atttccgcga agacgcatca tcagaaagtg gacttcaaca
     4441 tcttcgaggg aatgcaggtc aaaggcggcg catcgcatac ggtgagcggc ggcaagctcg
     4501 tctacgctcg cggcgacctg cgcgtggaac gcggcgcggg caaccacat
//
LOCUS       DASQJS010000131         7224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1638266, whole genome shotgun sequence.
ACCESSION   DASQJS010000131 DASQJS010000000
VERSION     DASQJS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638266"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..661)
                     /locus_tag="VEW08_05450"
     CDS             complement(35..661)
                     /locus_tag="VEW08_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40214.1"
                     /translation="MTIPVEFVLFGLMLLGVAVFHRHTLMAALLGLAAITAYKIFFGD
                     FYGQPGLLGLWFHVIEEWVILANLFALLVGFALLSRHFEESHVPHLMPRFLPDDWKGG
                     LALLAMVFVLSAFLDNIAAAIIGATVAGTIYRHKLHIGFLAAIVAASNAGGAGSVLGD
                     TTTTMMWLDGVSPLHVLHAYVAAIAAFAVFAIPASRQQQRFSPIERNP"
     gene            complement(658..2166)
                     /gene="hutH"
                     /locus_tag="VEW08_05455"
     CDS             complement(658..2166)
                     /gene="hutH"
                     /locus_tag="VEW08_05455"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HYJ40215.1"
                     /translation="MFTIGNPLNFSALRDLSHGGVTLTISEAAYAAIERGSRAVAEIV
                     KRGEPAYGVNTGFGRLAQTHIPIDQLELLQTNLVLSHAVGVGAPLSAPVVRLTLALKI
                     ATLARGYSGVRRELIDALVKLYNADVLPRIPSKGSVGASGDLAPLAHLSAVLLGVGEV
                     ECAGKIMSADEGLTRAGLTRIKLAAKEGLALLNGTQISTALALVNLFSIDNLYATALV
                     AGALSVDAAEGSASPFDARIHELRGHPGQIAAAAAYRELLAGSPINSSHRDCGKVQDP
                     YSLRCQPQVMGACSDQIEGARAVLLREANAVTDNPLVFADTGEVLSGGNFHAEPVAFA
                     ADNLALAVAEIGALSERRIALLIDATLSGLPPFLVRDGGVNSGFMIAHVTAAALASEN
                     KLLAHPASVDSLPTSANQEDHVSMATFAARKLGELSANTANILAIELLAAAQGVDLRA
                     PHLTSEKLQRVMREIRARVPHYDIDRYLAPDIAAMADAVNGGVIAAHAPLPR"
     gene            2249..4417
                     /locus_tag="VEW08_05460"
     CDS             2249..4417
                     /locus_tag="VEW08_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYJ40216.1"
                     /translation="MASARQATLFFSGLALFASLTACKPNMKETPPALADTAPVAAKK
                     PYQVVSPFGSREDEYYWLRDDKRENPEMLAYVKAENAYADAMLAHIKPLETKVYNEII
                     GRLQQDDSTVPYLMNGYWYFRRYETGKEYPIYSRRAGTQEAPEEILLNGNELAKGHDF
                     FEIGDIAISPNSKLMAWAEDTVGRRQYVVKVMDLATRRIFPITLSNVENNVIWAGDNQ
                     TFFYIEKDPKTLLGFRVRSHRLDSANHTDVAADPVVWTQQDESFYTQLYRTKDEKYLL
                     IHTQSTVSTEVLYADAASKKLEFKVFLPRERDHEYQVEHANGRWIVRTNFQAKNFRIV
                     EVPMGAEADRGKWKDVVAHRDDAFVDAFEVARNFLTIEEHSGGLRKLRIRPWNGGEDV
                     FVTADEQSYTMALDVNREFDSDKLRYTYQSMVTPKTTYDYDFKTAKRELLKREPVLGG
                     YDPANYANEFAWATARDGVRIPVSIVYRKTTPRDGTAPLLQIGYGAYGSTYDPEFSYL
                     PPSLLDRGFVVAIAHIRGGQEMGRAWYENGKLLNKKNSFTDFIDVTRWLAASKYSDPK
                     RAFALGRSAGGLLMGAIANMAPSDYAGIVTGVPFVDVITTMLDESIPLTTNEFDEWGN
                     PKKKEFYDYMLSYSPYDQLGAHDYPAMFVHSGLWDSQVQYFEPTKYVARLRARRTGTS
                     LVVLRTNMEAGHGGKSGRYEHFREYAEQYAFIIDQAGIKD"
     gene            4541..5635
                     /locus_tag="VEW08_05465"
     CDS             4541..5635
                     /locus_tag="VEW08_05465"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HYJ40217.1"
                     /translation="MPTRKETVAPAPTVGFEPVNWSKLPGWRADDALAAWPAIIASCS
                     VMHAKPEWQSFCGFVVAASPVDSDFVRAFLEQHLQPYRVLRVTGRKVGKTGLVTGYYE
                     PLLRGSRERSDEFATPLYRRPEDLLIVDLAEVIPELKGKRVRGRVEGNKVVPYYSRAA
                     SREATALAGHEIVWIDNALDAFMLEVQGSGRVQLATGETIRLQYEDQNGHPYRSIGKY
                     LADQGIMTLDQVNMPAIRAWLAANPQRLHEVLDINPSMVFFREAPQDDPAVGPKGAMA
                     VPLTAGRSIAVDPKFLKLGAPMFLATTEPTTRLPLSRLVVAQDTGGAIRGPIRADLFF
                     GFGSEAGAQAGMMKYDGEMWLLWPKQAPPP"
     gene            complement(5645..6181)
                     /locus_tag="VEW08_05470"
     CDS             complement(5645..6181)
                     /locus_tag="VEW08_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40218.1"
                     /translation="MKIAIVKCSAVALSMMLSAAAPAADAVGEEPAVWKALAEFIASD
                     NTSRPFKVLYFQSDFETGPLVTSSMTDPTRREYKELCGLSVSDAKAMVAQLQAVNAEA
                     VAFEASVAEPAGLKIGKKKAPRSRYVALSRVVFDPSNQHAWLAVDLGGVAGSIMRLDK
                     VSGQWSKTARCGAWVRSE"
     gene            complement(6419..6970)
                     /locus_tag="VEW08_05475"
     CDS             complement(6419..6970)
                     /locus_tag="VEW08_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40219.1"
                     /translation="MNTSKTKKLSRPASALTCVMLMLVANVARPGAAVLGEELAVLRT
                     IAAIVNRDASRPYERLYFESEFEGASFVATSLENPDRNQFCGLSRSEALAMVSELTIV
                     TDRRVQLDKEIAKSAGLKIGRWKDPRFPYVILSRVLFGPGKQYAWVAVELNGKAGSVM
                     RLDKVDGEWKKTSRCGGWVKYEN"
BASE COUNT         1438 a         2333 c         2202 g         1251 t
ORIGIN      
        1 tgatcacgcg cgcccaatcc acttcgacgc cgaattacgg atttctttcg atgggcgaga
       61 agcgctgctg ctgccgcgac gccggaatcg cgaacaccgc gaacgcggca atcgccgcca
      121 cataggcatg gaggacgtgg agcggcgaga caccgtcgag ccacatcatg gtcgtggtgg
      181 tgtcgcccaa cacgctgccg gcgccgccgg cattggaggc ggcgactatc gccgcgagaa
      241 aaccaatgtg cagcttgtgg cggtagatag tgcccgcaac cgtggcgcct ataatggccg
      301 cggcaatgtt gtcgagaaag gccgacaaca cgaacaccat cgccagcagc gcaaggcccc
      361 ctttccagtc atcgggcagg aaccgcggca tcaggtgcgg cacgtggctc tcttcgaaat
      421 gccgggacaa cagcgcaaag cccaccagca gcgcgaacag gttggcgagg atcacccatt
      481 cttcgatgac gtggaaccac aggccgagca ggccgggctg accatagaag tcgccgaaga
      541 aaatcttgta cgccgtgatc gccgcgaggc cgagcagcgc ggccatgagc gtgtgccggt
      601 gaaacacggc cacgccgagc agcatcaggc cgaagagcac gaactcgacg ggaatggtca
      661 ccgcggcaag ggtgcgtgcg ccgcgatcac gccgccgttg acggcatcgg ccatggcggc
      721 gatgtccggc gcgaggtagc ggtcgatgtc gtagtgcggc acgcgcgcgc ggatctcgcg
      781 catcacgcgc tgcagttttt ccgaagtcag gtgcggtgcg cgcaggtcca caccctgcgc
      841 cgcggccagc agctcgattg ccagtatgtt tgcagtgttg gcagaaagtt cgcccagctt
      901 gcgcgccgcg aacgtggcca tcgaaacgtg atcttcctgg ttcgccgacg tgggcagtga
      961 atcgacgctg gccgggtgcg ccagcagctt gttctcggac gcgagcgccg cggcggtgac
     1021 gtgcgcgatc atgaacccgg agttcacgcc gccgtccctg acgaggaagg gcggcaagcc
     1081 cgagagcgtg gcgtcgatga gcaacgcgat gcgccgctcg gacagtgcgc cgatctcggc
     1141 cacggccagc gcaaggttgt cagccgcgaa cgccacgggt tcggcgtgga aattgccgcc
     1201 ggagagtact tctcccgtat ccgcgaaaac gagcggattg tcggtgacgg cgttggcttc
     1261 gcgcagcagc accgcgcgcg cgccttcgat ctgatcgctg caggcgccca tgacctgcgg
     1321 ctggcagcgc aggctgtatg gatcctgcac cttgccgcaa tcccggtggc tggaattgat
     1381 cgggctgccg gctaacaact cccgataggc ggcggccgcc gcaatctgcc cgggatgccc
     1441 gcgcaattcg tggatgcgcg cgtcgaacgg actcgccgaa ccctcggcgg catccaccga
     1501 cagcgcgccg gcgacgagcg cggtggcgta caggttgtcg atggagaaaa gattgaccag
     1561 cgccagcgca gtcgagatct gcgtgccgtt tagcagtgcg agaccctcct tggcggcgag
     1621 cttgatgcgc gtgaggccgg cgcgcgtcag gccttcgtcg gcgctcatga tttttccggc
     1681 gcactcgact tcacccacgc ctaacaatac ggccgacagg tgtgcgagcg gcgcgaggtc
     1741 gccactggcg cctaccgaac ctttcgaagg tatgcgcggc agcacatcgg cgttgtagag
     1801 cttgaccagc gcatcgatga gctcgcggcg cacgcccgag tagccgcgcg ccagcgtggc
     1861 gatcttcagc gccagcgtga gccgcaccac cggcgcggac aatggcgcgc cgacgccgac
     1921 cgcgtgcgac agcaccaggt tggtctgcag caactcgagc tggtcaatgg gaatgtgggt
     1981 ttgcgcaagc cggccaaagc ccgtattcac gccataagcg ggctcgccgc gtttcacgat
     2041 ctcggccacg gccctggaac cgcgctcgat ggcagcgtaa gccgcctccg aaatcgtcaa
     2101 ggtgacaccg ccgtgcgaca aatcgcgcaa ggcggagaaa ttcagcggat tgccgatggt
     2161 gaacatcgcg gcatgatgcc tcaactgccc ccgccagatc agtccccacc ggctagaatg
     2221 agtgcatcac ccgaacaagg tttcccccat ggcatcagct cgccaggcca cgctcttctt
     2281 ttccggattg gccctcttcg cttcactgac agcctgcaaa ccgaacatga aagaaacacc
     2341 gcccgccctc gctgataccg ctcccgttgc ggcaaagaag ccctaccagg tcgtctcccc
     2401 ttttggcagc cgcgaagacg agtactactg gctgcgcgac gacaaacgcg agaacccgga
     2461 aatgctcgcg tacgtgaagg cggagaacgc ctacgccgat gccatgctgg cgcacatcaa
     2521 gccgctggaa acgaaggtct acaacgagat catcggccgc ctgcagcagg atgactccac
     2581 cgtgccgtat ctgatgaacg gctactggta cttccgccgc tacgaaaccg gcaaggaata
     2641 tcccatctat tcgcgccgtg ccgggacgca ggaggctccc gaggaaattc tgctgaacgg
     2701 caacgagctg gcgaagggtc acgatttctt cgagatcggc gacatcgcca tcagcccgaa
     2761 cagcaagctc atggcctggg cggaagacac cgtcggccgg cggcagtacg tcgtcaaggt
     2821 catggacctg gccacgcgca ggattttccc catcacgctg tccaacgtgg aaaacaacgt
     2881 catctgggcg ggcgacaacc agaccttctt ctacatcgag aaagatccga agaccctgct
     2941 cgggttccgc gtgcgcagcc atcgcctgga cagcgcgaac cacaccgacg tggccgcgga
     3001 tccggtggtc tggacccagc aagacgagag tttctacacg cagctctatc gcaccaagga
     3061 cgagaagtac ctgctcatcc acacgcagag caccgtgtcg accgaagtgt tgtatgcaga
     3121 cgccgcgtcg aagaagctgg aattcaaagt gttcctgcca cgcgagcggg accatgaata
     3181 ccaggtggag cacgccaacg gccgctggat cgtgcgcacc aacttccagg caaagaactt
     3241 ccgcatcgtc gaagtgccga tgggcgcaga ggcggaccgc ggcaagtgga aagacgtcgt
     3301 ggcgcatcgc gacgacgcct tcgtcgacgc attcgaagtt gcgcgcaatt tcctgaccat
     3361 cgaagaacat tcgggcggcc tgcgcaagtt gcgcattcgc ccgtggaatg gtggtgaaga
     3421 cgtcttcgtc acggcagatg aacagagcta caccatggcg ctcgacgtca accgcgagtt
     3481 cgacagcgac aagctgcgct acacctacca gtccatggtc acgccgaaga ccacttacga
     3541 ctacgacttc aagacggcca agcgcgaact gctgaagcgc gaacccgtgc tcggcggcta
     3601 cgacccagct aactacgcca acgagttcgc ctgggccacg gcgcgtgatg gcgtcaggat
     3661 cccggtgagt atcgtctacc gcaaaaccac accgcgcgac ggcaccgcgc cgttgctgca
     3721 gatcggctac ggggcttatg gatccaccta cgaccccgag ttctcctacc tgccgccgtc
     3781 gctgctcgat cgcgggttcg tcgtggccat cgcgcacatc cgcggcggcc aggagatggg
     3841 ccgcgcctgg tatgaaaacg gcaagctgct gaacaagaag aattcgttca cggatttcat
     3901 cgacgtcacg cgctggctcg cggccagcaa atacagcgac ccgaagcgtg cgtttgcgct
     3961 cggccgctcg gccggtggcc tgctcatggg cgccattgcc aacatggcac cctccgacta
     4021 cgcgggcatc gtcaccggcg ttccgttcgt ggacgtgatc accaccatgc tcgatgagag
     4081 cattccgctc accaccaacg agttcgacga gtggggcaat ccgaagaaga aggagttcta
     4141 cgactacatg ctgtcgtact cgccctacga tcagttgggc gcgcacgact accccgccat
     4201 gttcgtgcac tccggtctgt gggacagcca ggtgcaatac ttcgagccca ccaagtacgt
     4261 ggcgcgcctg cgcgcgcggc gcacgggaac cagtctcgtg gtattgcgga ccaacatgga
     4321 agctggccac ggtggcaagt cggggcgata cgagcacttc cgcgagtacg cagagcagta
     4381 cgccttcatc atcgaccagg ccggcatcaa ggattaatgg ggccaggcgc cgcagcggcg
     4441 ccgttcctga tttgccagca aaaggggacg cgcctcgaac gtgatgacac tctttaatcg
     4501 gttcgccgct tgcgcagcag tcgcccttct cgcggcttgc gtgcccacgc gcaaggaaac
     4561 tgtcgcgccc gcgccgaccg tgggcttcga gccggtgaac tggtcgaagc ttcccggctg
     4621 gcgcgccgac gatgcgctcg ccgcgtggcc ggcgatcatc gcctcctgca gcgtcatgca
     4681 cgccaaaccc gagtggcagt cgttctgtgg attcgtggtc gcggcgagcc cggttgattc
     4741 cgacttcgtg cgcgcattcc tcgagcagca cctccagccc tatcgcgttc tgcgcgtcac
     4801 cggacgcaag gtcgggaaaa ccggcctcgt taccggctac tacgaaccgc tgctacgcgg
     4861 ttcgcgtgaa cgtagcgacg aattcgcaac accgctgtac cggcgccccg aagacctgct
     4921 catcgttgac ctcgccgagg tcatccccga gctcaagggc aaacgcgtgc gcggacgcgt
     4981 ggaaggcaac aaggtggtgc cctattacag ccgcgctgcg tcgcgcgagg cgaccgcgct
     5041 cgcaggccac gaaatcgtct ggatcgacaa cgcgctcgat gcattcatgc tcgaagtaca
     5101 gggatccggc cgggttcagc tagctaccgg cgagaccatc cggctgcagt acgaggacca
     5161 gaacgggcat ccctaccgct cgatcggaaa gtatctcgcc gaccagggca tcatgactct
     5221 cgaccaggtg aacatgccgg cgatccgcgc gtggctggcg gccaatccgc agcgcttgca
     5281 cgaggtgctc gacatcaacc cgagcatggt ttttttccgc gaggctccgc aggatgaccc
     5341 cgccgtcgga ccgaaaggcg ccatggccgt gccgttgaca gccggacgct cgatcgccgt
     5401 ggacccaaag ttcctgaagt tgggagcgcc gatgttcctt gcgaccacgg aacccacaac
     5461 gcggcttccg ctgagtcgac tggtggtggc gcaggacacc ggtggcgcca tccgcggacc
     5521 cattcgtgcg gatctcttct ttggcttcgg cagtgaggcc ggcgctcagg cgggaatgat
     5581 gaagtacgac ggcgaaatgt ggctgctgtg gccgaagcag gcgccaccgc cgtgaatcac
     5641 cgcattactc tgatctgacc caggctccac atcgagcggt cttgctccac tgacccgaca
     5701 ccttgtcgag tcgcatgatg gagcctgcaa cgccacccag atcgaccgcg agccacgcat
     5761 gctggttgct ggggtcgaac accacgcgcg acagcgcgac atagcgcgac cgcggagcct
     5821 tcttctttcc gattttcaaa cctgcaggct ccgcaaccga cgcttcgaat gccacagctt
     5881 cggcgttgac cgcctggagc tgagcgacca tggccttcgc atccgaaacg gaaagcccgc
     5941 atagttcttt gtattccctg cgtgtgggat ccgtcatcga gctggtgacg agcggaccgg
     6001 tttcgaagtc cgactggaag tacagaacct tgaatggacg ggaggtgttg tcactcgcga
     6061 tgaattcggc caacgccttc cagaccgccg gctcttcgcc gacagcgtcg gccgcgggcg
     6121 ccgcggcgct cagcatcatc gacagcgcaa ccgcgctgca tttaacgata gcaatcttca
     6181 tggcggcaaa ttacgcaagc tgtccgcgcc atgccactgt cgttgcagca gtttgcgacc
     6241 tgcgtcggcg tcgcacccga tgtgcgcgga agattccgcg ccgcgccgcc gtgggggcga
     6301 gctcacgagg ttttacccta gtgcttcgcc gcatggcgat cggcccatct accggacaat
     6361 tgtgatcgct tgcacgcatg attggatcga tcctggaaaa ccttcagtcg ccctgacgtc
     6421 agttctcata cttgacccag ccaccacatc gcgaagtctt tttccattcg ccgtcgactt
     6481 tgtcgagccg catgacggaa cccgccttcc cattcaattc aaccgcgacc cacgcgtatt
     6541 gtttgcccgg accgaagagc acgcgcgaca ggattacgta ggggaagcga ggatctttcc
     6601 agcgtccgat cttcaagccc gccgacttcg caatttcctt gtcgagttgc acgcgccggt
     6661 cggtaactat cgtgagctcg gagaccatcg caagcgcctc cgagcgtgaa agcccgcaaa
     6721 attgattgcg atccggattc tcgagggaag tggcgacgaa cgatgcgcct tcgaactccg
     6781 actcgaaata gaggcgctcg tagggacggg aggcatcgcg attgacgatc gcggcaatcg
     6841 tccgcagcac cgcaagctct tcgccaagaa cggcggcgcc aggtctcgcg acattggcca
     6901 ccagcatcag catcacgcac gtgagggcgg acgccggtcg agaaagtttt tttgttttcg
     6961 atgtgttcat ggcggcggaa cctacgcaag ccattcgcac catgccacta ccaaatcagc
     7021 ggtttgcgaa ctgcgtcagc ctcgcacatt ttgtgcgcgc ggttttttgc catcgctctg
     7081 gcgcggtgcg caatgacact ttgtcagcga tgaatttttc tcacgcaagc atggatttca
     7141 tgcggcctct tgctccgcct tcagcaggtt cgcggcattt ttttcgaccc accggctgaa
     7201 caaacccgcc agccgcgaca gcga
//
LOCUS       DASQJS010000132         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1323977, whole genome shotgun sequence.
ACCESSION   DASQJS010000132 DASQJS010000000
VERSION     DASQJS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323977"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VEW08_05480"
     CDS             <1..247
                     /locus_tag="VEW08_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HYJ40220.1"
                     /translation="VLILDEPTRGIDIAAKQELMNEILDLARGGTAVLFISAEIDEVV
                     RVSDRILVLRDRNKAGELPRGADENAVYELIAASHKA"
     gene            244..1263
                     /locus_tag="VEW08_05485"
     CDS             244..1263
                     /locus_tag="VEW08_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ40221.1"
                     /translation="MKRLTQHMLFWPAITLALLLVASSLFNPSFLALTWRDGHLYGSV
                     VDILNRAAPLMVVSLGMTLVIAVRGLDISVGAVVAISAAVVALMIGGSFSATGTVQSA
                     HPLWVALTAALAVAAACGLWNGLLVVKAGMQPIVATLILMVAGRGIAQLLTGGQIITV
                     YYPPFFALGNGYLIGLPVALWIAGSLWLALHLVMSRTALGLFVRSIGGNPEAARVAGV
                     RSAAITLCVYTFCGLAAGVAGLIVSSNVKSADANNAGQLLELDAILAVTLGGTALTGG
                     RFTLAGTVIGALIIQTLTSMIYSLGVPPEVNLVVKATLVFAVMLLQSAEFRQLVARAA
                     RGSTT"
     gene            1260..>2006
                     /locus_tag="VEW08_05490"
     CDS             1260..>2006
                     /locus_tag="VEW08_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40222.1"
                     /translation="MNRRLLPLTVTIVLFAAMAAFGAIRYEGFLSLQVFLNLLIDNAF
                     LCVVAVGMTFVILSGGIDLSVGAVIALTTMVSATLLDAGWNPFAAMALVLVLGAAFGA
                     LQGFLIQRFNLAPFLVTLAGMFLARGCCYLISTESIPIDDTTYAAIAQARIPVGFGAS
                     LTIGGLIAVAVVGIAMRIAHSSEFGRTVYAIGGGESSARLMGLAVDSTKIGVYLLSGA
                     CAALGGIVMTFYMLSGYSLHAVGLELDAIAA"
BASE COUNT          281 a          670 c          650 g          405 t
ORIGIN      
        1 cgtgctgata ctcgatgaac ccacgcgcgg catcgacatc gccgccaagc aggaattgat
       61 gaacgagatc ctggatctcg cgcgcggcgg cacggccgtg ttgttcattt ccgcggaaat
      121 cgacgaagtg gtgcgcgtgt cggaccgcat cctggtgctc cgcgaccgca acaaggcggg
      181 cgagttgccg cgcggcgcgg atgaaaatgc cgtctatgaa ctcatcgccg cgagccacaa
      241 ggcatgaagc gcctcacgca gcacatgctg ttctggccgg cgattacgct ggcgctgctg
      301 ctggtggcga gcagtctctt caatccttcc ttcctcgcgc tcacctggcg cgacggccac
      361 ttgtacggca gcgtcgtcga tatcctgaac cgcgccgcac cgctcatggt ggtgtcgctc
      421 ggcatgaccc tggtgatcgc ggtacggggt ctcgacatct cggtgggcgc cgtcgtcgcc
      481 atttccgccg cggtcgtcgc actcatgatc ggtggcagct tcagcgcgac gggcacagtg
      541 caaagcgctc atccgctgtg ggtcgcgctc acggccgcgc tggcggtcgc ggcagcttgc
      601 ggactctgga acgggttgtt agtggtcaag gccggcatgc agcccatcgt cgcgacgctc
      661 attctcatgg tggccggccg cggcatcgcg cagctgctca cgggcggcca gatcatcacc
      721 gtgtactacc cgccgttctt cgcgctcggc aacggttact tgattggcct gccggtggca
      781 ttgtggatcg cgggctcgtt gtggttggca ctgcacctcg tcatgtcgcg caccgcgctc
      841 gggttgttcg tgcgttccat cggtggcaac ccggaggcgg cgcgcgtcgc cggcgtgcgt
      901 tccgcggcga tcacgttgtg cgtgtacacc ttttgcggcc tggcagccgg cgtcgcaggg
      961 ctcatcgtga gctccaacgt gaaaagcgcc gatgccaaca atgccgggca gctgctcgag
     1021 ctcgacgcca tcctcgccgt cacgctcggc ggcaccgcgc tcacgggcgg ccggttcact
     1081 ctcgccggca cggtgatcgg cgcgctgatc atccagacgc tcacctcgat gatctactcg
     1141 ctcggtgtac cgccggaagt gaacctggtc gtgaaagcaa cgctggtctt cgcggtgatg
     1201 ctgctgcaat cggcggagtt caggcagctc gttgcacgag ccgcgcgcgg gagcacgacg
     1261 tgaatcgccg cctgctgcca ctcaccgtca cgatcgtgct gttcgccgcc atggcggctt
     1321 tcggcgccat ccgctacgag ggctttctct cgctgcaggt gttcctgaat ctgctcatcg
     1381 acaatgcctt cctgtgcgtc gtcgcggtgg gcatgacctt cgtcatcctg tcgggcggta
     1441 tcgacctgtc ggtgggtgcg gtcatcgcgc tgacgacgat ggtctccgcc acgctgctcg
     1501 acgccggctg gaaccccttt gccgccatgg cgctggtgct ggtgctcggc gcggccttcg
     1561 gcgcgctgca gggattcctc atccagcgct tcaatctcgc gccgttcctg gtgacgctgg
     1621 ccgggatgtt cctggcgcgc ggctgctgct atctcatcag taccgagtcc atcccgatcg
     1681 acgacaccac ctacgccgcc attgcccagg cgcgcatccc ggtgggcttc ggcgcatcgc
     1741 tgaccatcgg tggcctcatc gcagtggccg tggtggggat cgcgatgcgc atcgcacact
     1801 cgagcgagtt cggacgcacg gtgtacgcca tcggcggcgg tgaatcctcc gcgcggctca
     1861 tgggtctcgc cgtcgattcc accaagatcg gcgtctatct actgagcggt gcatgtgcgg
     1921 cgctcggcgg catcgtcatg acgttctaca tgctctcggg ctacagcctg cacgcagtgg
     1981 gcctggagct cgacgccatt gccgcc
//
LOCUS       DASQJS010000133        10926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4151725, whole genome shotgun sequence.
ACCESSION   DASQJS010000133 DASQJS010000000
VERSION     DASQJS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4151725"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VEW08_05495"
     CDS             complement(<1..221)
                     /locus_tag="VEW08_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ40223.1"
                     /translation="MTESAKNSRSILVTGSSRGIGRAIAVRLASSGFQLILHCRENLA
                     AAEETRATIVAAGGLNPRILRFDIADRTG"
     gene            complement(218..679)
                     /locus_tag="VEW08_05500"
     CDS             complement(218..679)
                     /locus_tag="VEW08_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40224.1"
                     /translation="MTTTPTLTIDAILPHVGRMRLLDELHDQGPEHVTCGVTIHAGTM
                     FCDGVHGVPAWVGLEYMAQTACAFSGVEEVRAGLRPSIGLLLGTRSYQCSTEWFPLGA
                     TLHIRADLLMRDDNDLVAFACSIHEGDRLLARGDLKAYRPKDVLALVGGTR"
     gene            complement(685..1257)
                     /locus_tag="VEW08_05505"
     CDS             complement(685..1257)
                     /locus_tag="VEW08_05505"
                     /inference="COORDINATES: protein motif:HMM:NF023089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HYJ40225.1"
                     /translation="MKPAGSLFARLSGCVALLALGACVGSPPPKAAVSLRPLVAPESL
                     GGERVVNQVVRGAFGARELTFNCVVTVKDGAMTLVGMNSLGVRLFTIRYDGKAVHSET
                     TPALQGSFVPERLLADMQLVFWPLSSLEKPMREAGWQLSESAPGIRRLRHGDQLTAEA
                     HYGTEDPWSGRSWLVNFEFGYSLQIDSQPM"
     gene            complement(1254..2471)
                     /locus_tag="VEW08_05510"
     CDS             complement(1254..2471)
                     /locus_tag="VEW08_05510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYJ40226.1"
                     /translation="MLIIGAGPAGSMSAALLASKGYEVLVLEKQRFPRFSIGESLLAS
                     TAELMNEAGMLEAVMACGFQYKNGAAFVRDELYSEFNFADKFSAGYPFTFQVPRAQFD
                     QVLANEAARRGATIRHEVEITAIDVSGERPMVTAKSGDEITTHRPRFVLDASGFGRTL
                     PRLLDLDRPSDFPERAALFVHVKDNIVSGEFDRQKIRVGVHPTKGDVWSWLIPFSNGN
                     ASLGVVASREHHAKFSGTPEERLWQLIAEEPPLRKLLKDAHTVRPVGEIVGYAAKVSR
                     LHGAGFALLGNAAEFLDPVFSSGVTIAMKSAKLAAELLDRQFRGEPADWQTQFAEPLM
                     YGVETFRSFVDGWYDGDLQDVIFFEHKDPVIRRMICSVLAGYAWDPKNPYTGPQSARR
                     LRALASTVRAVRS"
     gene            complement(2473..4824)
                     /locus_tag="VEW08_05515"
     CDS             complement(2473..4824)
                     /locus_tag="VEW08_05515"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYJ40227.1"
                     /translation="MTPGASLRVRFIGWSVLMLAMLGVFAFRVLPEPRVETDILALLP
                     QAQTDRGFDAALDEFSAQLARQQIFLIGSDTLADAKLAAAAFANTLETSGAFTRIQWQ
                     LDTDLTQRAAVYLAHRAFLVSPDDRAALEAGATERLVRRALRTAYTPTGLMQPLGLAE
                     DPLGFMNDFLRASTGVAGNARLEGNVLVAARDGREFVLVLTENAGHPFASATQERVIP
                     AFARARLAAQQAIDAPVEIVGSGAIQHASAAAVRAAHEVTTFGAIESIAVVLLLLLVF
                     GSMRPLLLSVLTLAFAITAAFTTVHLVFGEVHILALVFGSSLIGSVIDYSIHFFADRF
                     RDPEHWTPASAVPHVGPAILLGLTTTLVGYLVLAAVPFPGLEQIAVFCMTGLVVGCGC
                     VLCLYPVLARARGKLPRFGPVAGAAIDRMLRAWRWTRLELIVLAALAVVIGLGLARVR
                     IQDDVKALQQSPAQLVTEEQRVREVLGSGVETRFFLVSGDSAQAVLETEERLTHALDA
                     LVRAGDIASYKAVGTGVPSLELQRRNHELLATQVYAPGALLDRVMTELGFDPAAIERR
                     RVEFQAADAPLPVDEWLASPASQGARHLWLGEVGSRFATVVTLGGIANVESLKQLQLP
                     GVLLIDRIAQTTDTLSRYRYAMTLLLAAVYAVAALILLLRFGWRDALRMLLPSVAATL
                     TTVGIFGWLGVPFNLFTLLALWLVLGLGIDYGIFLRHGRDHRVTAILSVTLSACTTLI
                     AFGLLALSATPFIRSIGLTLLFAITLSWLFALLACMTENEIHG"
     gene            complement(4821..5504)
                     /locus_tag="VEW08_05520"
     CDS             complement(4821..5504)
                     /locus_tag="VEW08_05520"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HYJ40228.1"
                     /translation="MRSRRVMWCLCALFVMPAASGAPAADPVAHPLTDEAQLRSLLSR
                     PASKLRNAQVLTGKFQHSRHLSEIPKPLVAHGEFTFVRDLGVYWHTRQPFDSVVVLTG
                     TGLAQSDEGGVVQRISADEQPGVRLITNIFMALFTLDTQGLARDFDLSGGVADEKQNR
                     WIIGLKPRAKAIAGVFKEATVAGADDVEQVVLTDARGDRTVIDLNGITYSIDPPGADV
                     RALFALPRP"
     gene            complement(5501..5914)
                     /locus_tag="VEW08_05525"
     CDS             complement(5501..5914)
                     /locus_tag="VEW08_05525"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HYJ40229.1"
                     /translation="MTVREASVAITVPFHDVDSIGVAWHGHYAKYFEIARCSLLDSFN
                     YGYNAMRESGFSWPVIDMRIRYVKGVTFGQRILVKATLREWENRLLIAYLITDEASGE
                     RLTKGNTVQVAVDMKNGEMCFVSPPVLFERLGLAP"
     gene            complement(5911..6666)
                     /locus_tag="VEW08_05530"
     CDS             complement(5911..6666)
                     /locus_tag="VEW08_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYJ40230.1"
                     /translation="MKSCIVIPFYNHAGAIAHVVDALEPLDLPCFIVDDGSDAESQRV
                     LSLIAMRKSWVRLLRLPQNSGKGAAVIAGCDAALAAGFTHALQIDADGQHDFADAARL
                     LEVARQDPAAMVSGEAIYDSTVPRSRRYGRYLTHVWVWINTLSFDIRDSMCGLRVYPL
                     AATCEIWRRHRIGKRMDFDTEIMVRLHWSGVHIVAVPTRVTYPRDGVSHFRMLEDNVF
                     ISRMHTRLFFGMLWRLPALLGRRLMHRGPKAAA"
     gene            complement(6684..7025)
                     /locus_tag="VEW08_05535"
     CDS             complement(6684..7025)
                     /locus_tag="VEW08_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40231.1"
                     /translation="MLVLPDIDALERAQDHLNLRLIVAGTNLYFDGHFPGCPVLPGVV
                     QVGWAIEFARLHIPFSGRFRSLAAVKFMRVIQPNAALTLRLAADAAKRQLSFEYRLSG
                     EPCSSGRVLFH"
     gene            complement(7035..7304)
                     /locus_tag="VEW08_05540"
     CDS             complement(7035..7304)
                     /locus_tag="VEW08_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HYJ40232.1"
                     /translation="MSVQAKSREEILEHLRVVLSDQFELKAGQIVPEANLYTDLDLDS
                     IDAVDLFLTLKEITGRKIQPQAFKDVRTVNDVIDAIQTLTSNPAG"
     gene            complement(7330..8175)
                     /locus_tag="VEW08_05545"
     CDS             complement(7330..8175)
                     /locus_tag="VEW08_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYJ40233.1"
                     /translation="MSGADRKLNHVWRIFATGFVFMLFGIGALVISVTIFPVLRLSTW
                     KADTSRRRIQHAMQLTFRAFVETMRVLGIFTYRVEGAERLREPGRLIVANHPTLIDVV
                     LLVSQMPEVDCIVKRSLWRNPFLRWPVSWAGYLPAASGEELIEECAATLRHGRSLLVF
                     PEGTRSVTGRPLHMQRGAAHIALAADSDILPVTISCDPPTLSKGNPWYRVPAQRFHMQ
                     VVVGEPMAARDFIRDDEAPVRTARRITQWMLTQFDAARATQPGHVGATGLPWLAEPAQ
                     QHQVR"
     gene            8341..8640
                     /locus_tag="VEW08_05550"
     CDS             8341..8640
                     /locus_tag="VEW08_05550"
                     /inference="COORDINATES: protein motif:HMM:NF006617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYJ40234.1"
                     /translation="MNTEAATSPQTAEELLMAQRLVQVLGLEHIAPDAIDPAAPLFGS
                     AVDSIGLDSIDALEIALMIQQHYGVELRSEDVAARAAFGSLRLLTEFALRSRPAG"
     gene            8676..9269
                     /locus_tag="VEW08_05555"
     CDS             8676..9269
                     /locus_tag="VEW08_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40235.1"
                     /translation="MFWLALGYPLLAHLAVLTHDRRLEWLALVWLLGIAMGGALIQRR
                     LWAWGALLAGAGLLYWLVIEGNGLYALYIPAAAIPAALFMLFALSLRAGEIPLVTRIA
                     RLMHDGPFPDDLVVYTRHVTQLWCGVCAAMFLSAVLLALFASPGLWSLMTNVIHYVVL
                     GAVFVLEYGYRRWRYRHHEHSGLFQYLRRLTRTKLKV"
     gene            9275..10570
                     /locus_tag="VEW08_05560"
     CDS             9275..10570
                     /locus_tag="VEW08_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYJ40236.1"
                     /translation="MQALRLLAVDGLDAPIAYYRNATVRAGQFVAHVEHVARALPRAA
                     HVINLSENRYHFLLGWVAACLRDQVTLLPSGRTDDVLAGLRHQYPDHHTIDGESIERL
                     LSQAPTSGSKTIPANWQLPAERIVAIAFTSGSTGDPTPHPKTWGSLFHNSRLAAAEVL
                     GGPNRALVSTVPPQHMYGLEASLLSALTAQSVLFDDKPFYPADVRRALEAMTAPRVLV
                     TTPAHLKVLADADIELPPLDRVVSATAPLPVELARRIETGWNTQLVEIYGCTEAGVMA
                     HRRPAQTESWRTFAGGTMAAASGAAEYFAPQLGGAVPLQDVLELRGPTEFFLRGRTAD
                     MIKVAGKRASLQELTRQILSLPGVEDAVVFLPDTDARPAAAVVAPGLTAAAILRELRT
                     RLDGVFVPRPLVIVDELPRNSVGKLRREELLGLLAKARA"
     gene            10567..10890
                     /locus_tag="VEW08_05565"
     CDS             10567..10890
                     /locus_tag="VEW08_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40237.1"
                     /translation="MNDAITNEFVVPADHPSLAGHFPGQPVVPAVVLLDAVLAAIRTR
                     GDFVLRSIPVAKFLQPVLPDERVNLRIHLISTDGAQLRASFAGMRAAVLVFEGSFIVS
                     ASSES"
BASE COUNT         1990 a         3613 c         3500 g         1823 t
ORIGIN      
        1 cagccggtac ggtccgcgat gtcgaagcgc aggatgcgcg ggttcagccc gccggcggcc
       61 acgatggtgg cgcgcgtctc ctcggcggcc gcgaggtttt cgcggcagtg caggatcagc
      121 tgaaaaccgc tggacgccag gcgcacggca atggcgcggc cgatgccccg gctggagccg
      181 gtcaccagga tgctgcgact gttcttggcg ctctcggtca tcgtgttcct cccaccagcg
      241 cgagcacatc tttcggacgg tacgccttga ggtcgccgcg cgcgagcagg cggtctccct
      301 catgaatgga gcaggcgaac gcgacgagat cgttatcgtc gcgcatcagg aggtcggcgc
      361 gaatgtgcag cgtggcgccc aacgggaacc actcggtgga acactggtaa ctgcgcgttc
      421 ccagcaacaa gccgatgctc ggcctgaggc ccgcgcgcac ctcttcgacg ccggaaaatg
      481 cgcaggccgt ctgcgccatg tattcgagcc ccacccatgc gggcacacca tggacgccat
      541 cgcagaacat cgtgccggcg tggatcgtga cgccgcaggt cacatgctcc ggaccctggt
      601 cgtgcagttc gtcgagcagc cgcatccggc ccacgtgagg caggatggcg tcgatcgtca
      661 gtgtgggcgt cgttgtcatt ggagctacat cggctggctg tcgatctgca gcgaatatcc
      721 aaactcgaag ttcacgagcc agcttcggcc gctccacggg tcttcagtgc cgtagtgcgc
      781 ttcggcagtc agttgatcgc cgtggcgcag ccggcgaatg ccgggcgcgg attcgctcaa
      841 ctgccatccc gcctcgcgca tcggcttctc gaggctcgac agcggccaga agacgagctg
      901 catgtccgcg agcaggcgtt ccggcacgaa cgacccctgc agggccggtg tggtttcgga
      961 atgcaccgcc ttgccgtcat atcgaatggt gaagagccgc acgccgagcg aattcatgcc
     1021 gacgagcgtc atggcgccat ccttgacggt caccacgcag ttgaacgtga gttcgcgcgc
     1081 accaaatgcg ccgcgcacca cctggttgac gacccgttcg ccgccgagcg attcgggtgc
     1141 cacgagcggc cgaagagaga ccgcagcctt gggtggcggc gaacccacgc acgcgccgag
     1201 cgccagcagc gcgacgcatc cggacaatcg cgcgaacaaa ctacccgcgg gtttcatgac
     1261 ctcaccgccc tgaccgtact cgccagcgca cgcagacggc gcgcggattg cggacccgtg
     1321 tacggatttt tcggatccca cgcataaccg gcaagcaccg agcagatcat gcgccggatc
     1381 acgggatcct tgtgctcgaa gaagatgacg tcctgcaggt cgccgtcgta ccagccatcg
     1441 acgaaactgc ggaacgtttc gacgccgtac atgagcggct ccgcgaactg cgtctgccag
     1501 tcggccggtt cgccgcggaa ctgtcgatca agcaactcgg ccgccagttt cgcggacttc
     1561 atggcgatgg tgacgcccga ggaaaacacg ggatcgagaa attccgccgc gttgccgagc
     1621 agcgcgaaac ccgcgccgtg aaggcggctc actttcgcgg cgtatcccac gatctcgccc
     1681 accggacgca cggtgtgcgc gtccttcagc aacttacgca gcggcggttc ttcggcgatc
     1741 aattgccaca gccgttcttc gggcgtgccg ctgaacttcg catggtgttc gcgcgaggcg
     1801 accacgccga gcgaggcatt gccgttcgaa aacggaatca gccacgacca gacgtctccc
     1861 ttcgtcgggt gcactcctac gcggatcttc tgccggtcga actcgccgga aacgatgttg
     1921 tccttgacgt gcacgaacag cgccgcgcgt tcggggaagt cggacggccg atccaggtcg
     1981 agcaaccgcg gcagcgtgcg gccgaaaccg ctggcgtcga gtacgaaccg cggccggtgg
     2041 gtcgtgattt catcaccgga tttcgccgtg accatcgggc gctcgccgga cacatcgatc
     2101 gccgtgattt ccacttcgtg acggatggtc gcgccgcgcc gcgccgcttc attggcgagc
     2161 acctgatcga actgcgcgcg cggaacctgg aacgtgaacg gataacccgc cgagaacttg
     2221 tcggcgaagt tgaattccga atacagctcg tcgcgcacga acgccgcacc gttcttgtat
     2281 tgaaagccac aggccatgac cgcttcgagc atgcccgcct cgttcatcag ttcggcggtg
     2341 ctcgcgagca ggctctcgcc gatcgagaag cgcgggaagc gctgcttctc gagcacgagc
     2401 acttcgtacc ctttggacgc cagcaatgcc gcggacatgg aaccggcggg gcccgctccg
     2461 atgatcaaca catcaaccat gaatctcgtt ctccgtcatg caggccagga gtgcaaacaa
     2521 ccacgataac gtgatcgcaa agagcagcgt cagaccgatg ctgcggatga aaggggttgc
     2581 gctcaacgcc agcagtccga acgcgatgag cgtggtgcag gcggacagcg tgacgctgag
     2641 gatggccgtg acgcggtgat cccgcccatg gcgcaggaag atcccgtagt cgattccgag
     2701 ccccagcacc agccataacg cgagcagcgt gaagagattg aagggcacac cgagccagcc
     2761 gaaaattccg accgtcgtca gagtagccgc cacggacggc aacaacatgc gaagggcgtc
     2821 gcgccagccg aagcgcagca ggagtatcaa cgccgcgacc gcatacaccg ccgccagcag
     2881 cagcgtcatc gcatatcgat agcggctcag cgtgtccgtc gtctgcgcga ttcgatcgat
     2941 gagcaggacg cccggtagtt gcagctgctt cagcgactcg acgttggcaa tgccgccgag
     3001 cgtcaccacg gtggcgaaac gcgaccccac ctcgccgagc cacagatgcc gcgcgccttg
     3061 cgacgccggg ctggccagcc attcgtccac cggaagcggc gcatccgcgg cctggaattc
     3121 gacgcgacgc cgttcaatgg ccgccggatc gaagccgagc tcggtcatca cgcgatcgag
     3181 cagcgcgccg ggtgcgtaga cctgcgtcgc aagcagctcg tgattgcggc gctgcagttc
     3241 gagtgatgga acgccggtgc cgaccgcttt gtaggaagcg atatcgcccg cacgcaccag
     3301 cgcgtcgagc gcatgcgtga gacgctcttc cgtctcgagc actgcctgcg ccgaatcgcc
     3361 cgacaccagg aagaagcgcg tttcgacgcc actgcccagc acctcgcgca cgcgctgttc
     3421 ttctgtcacg agttgcgccg gtgattgctg caacgctttg acgtcgtcct gaatccggac
     3481 acgcgcgagt cccaacccga tcaccacggc cagcgccgcc agcacgatca actcgaggcg
     3541 cgtccagcgc catgcgcgca gcatgcgatc gatcgcagcc cctgcgaccg ggccgaacct
     3601 aggcagcttg ccgcgggcgc gagcgagcac cgggtacaaa cacagcacgc agccgcagcc
     3661 cacgacaaga ccggtcatgc aaaacaccgc gatctgctcg agcccgggaa acggcacggc
     3721 ggcgagcacg aggtaaccca ccagcgtcgt cgtgaggccg agcaggatcg ccggacccac
     3781 gtgcggcacg gccgacgccg gcgtccagtg ctccggatcc cggaagcggt cggcgaagaa
     3841 gtggatcgag tagtcgatga cgctgccgat gaggctcgaa ccgaatacca gcgcgaggat
     3901 gtgcacttca ccgaagacca ggtgcaccgt ggtgaaagcc gcagtgatcg cgaacgccag
     3961 ggtcaggacg ctgagcaaca gcggacgcat cgaaccgaat accagcagca gcagcaatac
     4021 caccgcgatc gattcgatcg cgccgaacgt cgtcacctca tgcgcagctc gcacggccgc
     4081 ggcgcttgcg tgctggatgg cgcccgaacc gactatctcc accggcgcat cgatcgcctg
     4141 ctgcgccgcg agccgcgcac gcgcgaaggc gggaatcacg cgttcctgcg tcgcggaggc
     4201 aaatggatgt cccgcatttt cggtgagcac cagcacgaac tcgcggccgt cacgcgccgc
     4261 gaccagcacg ttgccttcca ggcgcgcatt gcctgcgact ccggtgctgg cgcgcaggaa
     4321 atcgttcatg aaacccagcg gatcctcggc gaggcccaac ggctgcatga gacccgtggg
     4381 cgtgtatgcg gtgcgcagcg cgcgcctcac gagccgctcg gtcgcgcccg cctcgagtgc
     4441 cgcacggtcg tccggcgaaa ccaggaacgc gcgatgcgcc aggtagactg cggcacgctg
     4501 cgtcagatcc gtatcgagct gccactgaat gcgcgtgaat gcgccggaag tctcgagcgt
     4561 gttcgcgaac gcggcggccg ccagtttcgc gtcggcgagc gtgtcgctgc cgatcaggaa
     4621 aatctgctgg cgcgcgagtt gcgcggagaa ttcatccagc gccgcatcga aaccccgatc
     4681 cgtttgagcc tgcggcagca gcgccaggat gtccgtctcc acacggggct ccggcagtac
     4741 gcggaatgcg aacaccccca gcatggcgag catcaatacg ctccagccga tgaagcgcac
     4801 gcgcagggac gcgcccggcg tcatggccgt ggaagcgcga acagggcccg cacgtcggcg
     4861 ccgggagggt cgatagagta agtgatgccg ttgagatcga tcacggtgcg atcgccacgc
     4921 gcgtcggtga gcacgacctg ctccacgtca tcggcgcccg cgacggtggc ctctttgaac
     4981 acgccggcga tggccttcgc tcgcggcttg agtccgatga tccagcggtt ctgtttctcg
     5041 tccgccacgc caccggagag atcgaagtcg cgcgccaggc cctgggtgtc gagcgtgaag
     5101 agcgccatga agatgttcgt gatgaggcgc accccgggct gctcgtccgc ggaaatccgc
     5161 tgcactacac ccccctcgtc cgactgcgcg agacccgtgc cggtgagcac caccaccgaa
     5221 tcgaacggct gccgcgtgtg ccagtacaca ccgagatcgc gcacgaatgt gaattcgccg
     5281 tgcgcgacga gtggcttggg aatttcgctc aggtgccggc tgtgctggaa cttgccagtg
     5341 agcacttgcg cgttgcgcag cttgctcgcc ggacgcgaca acagcgagcg aagttgagcc
     5401 tcatcagtca atggatgcgc gaccggatcg gcggcgggcg caccagatgc ggcaggcatc
     5461 acgaacaacg cgcacaggca ccacatcacg cgccgactcc tcatggcgcc agccccaggc
     5521 gttcgaacag cacgggcggc gacacgaaac acatctcgcc gttcttcatg tcgacggcaa
     5581 cctgcaccgt gttgcccttg gtcagccgct cgccgctcgc ctcgtcggtg atcaggtagg
     5641 cgatgagcaa ccggttttcc cattcacgca aagtggcctt gacgaggatg cgctgcccga
     5701 acgtcacgcc cttcacgtag cggatgcgca tgtcgatcac cggccagctg aaccctgact
     5761 cccgcatcgc gttgtagccg taattgaaac tgtcgagcag cgagcagcgc gcgatttcga
     5821 aatacttggc gtagtggccg tgccaggcca cgccgatgga atcgacgtcg tggaacggca
     5881 ccgtgatcgc gacgctggct tcgcgcacgg tcatgcggcg gccttcggac cgcggtgcat
     5941 cagccgccgg ccgagcagcg ccggcaatcg ccacaacatg ccgaagaaga ggcgcgtgtg
     6001 catgcggctg atgaagacgt tgtcttccag catcctgaag tgcgagacgc cgtcgcgggg
     6061 ataggtcacg cgcgtcggaa ccgcgacgat gtgtacgccg ctccagtgca gccgcaccat
     6121 gatctcggtg tcgaaatcca tgcgcttgcc gatgcgatgc cgccgccaga tttcgcaggt
     6181 ggccgcgagt ggatagacgc gaaggccaca catcgaatcg cggatgtcga acgacagcgt
     6241 gttgatccac acccacacgt gcgtgagata gcggccgtag cgccgtgagc gaggcacggt
     6301 ggaatcgtag atggcctcgc cgctcaccat cgcggccgga tcctgacggg ccacttccag
     6361 caggcgcgcc gcatcggcga aatcgtgctg cccatcggca tcgatctgca gcgcatgcgt
     6421 gaagcccgcg gccagcgccg cgtcgcatcc ggcgatgacg gcagcgccct tcccgctgtt
     6481 ctgcggcagg cggaggagcc ttacccagct cttgcgcatt gcaatcagcg acagtacccg
     6541 ctgcgactcg gcatccgacc cgtcatcgac gatgaagcag ggcaggtcga gaggctcgag
     6601 ggcgtccacc acgtgcgcga tggcgcccgc gtggttgtag aacgggatga cgatgcagct
     6661 tttcattgtt caatggaaag ggttcaatgg aacagcacgc ggccactcga acacggttcg
     6721 ccggaaagcc ggtactcgaa ggacagctgg cgcttcgccg catccgcggc cagccgcagt
     6781 gtcaaggcag cgttcggctg gatgacacgc atgaacttca ccgccgccag cgagcggaaa
     6841 cggccggaaa aagggatgtg caggcgcgcg aactcgatgg cccacccgac ctgcacgacg
     6901 ccgggcagca cgggacagcc ggggaagtgg ccgtcgaagt agagattggt gcccgcgacg
     6961 atcaatcgca gattcagatg atcctgcgcg cgctcgagcg catcgatatc cggaagcacg
     7021 agcacgatgg cgtctcagcc cgccggattg gacgtcagcg tctggatcgc atcgatgacg
     7081 tcgttgaccg tgcgaacgtc cttgaatgcc tgcggttgaa tcttccggcc ggtgatttcc
     7141 ttgagggtca ggaacaggtc gacggcgtcg atgctgtcga ggtcgaggtc ggtgtataga
     7201 ttggcctcgg gcacgatctg cccggccttg agctcgaatt gatcactcag caccactcgc
     7261 agatgttcca ggatttcttc acgggatttc gcttgcactg acatctcgac ttctctatct
     7321 accaattctt tatctgacct ggtgctgctg cgcgggctcc gccagccacg gcaacccggt
     7381 ggcacccacg tgacccggct gcgttgcccg cgcggcatcg aattgcgtga gcatccactg
     7441 ggtgattcgc cgcgcggtcc gcaccggcgc ctcgtcgtcg cggatgaaat cgcgggcggc
     7501 catcggttcg ccgacgacca cctgcatgtg gaagcgctgg gcgggaacgc ggtaccacgg
     7561 atttcctttg gacagcgtcg gcggatcgca ggagatcgtg accgggagaa tatcggaatc
     7621 ggcggcgagt gcgatgtgcg ccgcgccgcg ctgcatgtgc agtggcctgc ccgtcacgct
     7681 tcgcgtgccc tcggggaaca cgagcaacga acggccgtgt cgcaacgtcg ccgcgcactc
     7741 ttcgatgagc tcttcgcccg aggcggccgg tagatagcca gcccacgaca ccggccagcg
     7801 caggaacgga ttgcgccaca ggctccgctt gacgatgcag tcgacctccg gcatctgcga
     7861 gaccagcaac acgacatcga tgagcgtggg atggttggcg acgatcaggc gccccggttc
     7921 gcgcaggcgc tcggcgccct cgacgcgata ggtgaagatg ccgagcacgc gcatggtttc
     7981 cacgaacgcg cggaacgtga gctgcatggc atgttgaatg cggcgacggg aggtatccgc
     8041 cttccacgtc gacagccgca acacggggaa gatcgtcacg ctgatgacca gtgcgccgat
     8101 tccgaacagc atgaacacga acccagtggc gaatatccgc cacacatgat tgagtttgcg
     8161 gtccgctccc gacattgaaa tgcaccctcc cgcccgcgct gccgtcatga atctcgttgt
     8221 aatccgcgtc gatgcaatcg cgcgatgtgg tgccttgagt atagactgtc gcgccgcgcg
     8281 cgtgaccccg gcagacccca tcccagatga accactacta gattgtcacc cacagacaac
     8341 atgaacaccg aagccgccac gtccccgcaa accgccgaag aattgctcat ggcccagcgc
     8401 ctggtgcagg tcctcggtct cgaacatatt gctccagatg caatcgatcc tgccgcgccc
     8461 ctctttggca gcgccgtgga cagcatcggg ctcgattcga tcgacgcact ggaaattgcc
     8521 ctcatgatcc agcagcacta cggcgtggag ctgcggtccg aagatgtcgc ggccagggcc
     8581 gcgttcggtt cgctgcgtct gctgaccgaa ttcgcgctgc gcagccgccc cgcgggctga
     8641 tctcgccggg gcgaactgac cgccggctcc gccacatgtt ctggctcgca ctcggttatc
     8701 ccctcctggc tcacctggcc gtgctcacgc acgatcgacg gctcgagtgg ctcgcgctcg
     8761 tctggttgct cggcatcgcc atgggcggcg cactgatcca gcgtcgcctg tgggcctggg
     8821 gcgcgctgct ggccggcgca gggctcctgt actggctggt catcgaaggt aacggtctgt
     8881 acgcactcta cattccggcg gccgccatcc ctgccgcgtt gttcatgctg ttcgcgctgt
     8941 cgctgcgggc gggcgagatt ccgctggtga cacgcatcgc gcgcctcatg cacgacggcc
     9001 cgtttcccga cgacctggtg gtttacacgc gccacgtgac gcagctctgg tgtggcgtct
     9061 gcgccgccat gttcctctcg gcggtgctgc tggcgctgtt tgcatcgccc gggttgtggt
     9121 cgctcatgac caacgtgatt cattatgtcg tcctcggcgc ggtgttcgtc ctggagtacg
     9181 ggtaccggcg ctggcgctac cgccaccatg agcattccgg cctcttccag tatctgcggc
     9241 gcctgacgcg caccaaactc aaggtctgaa gacgatgcag gcgcttcgat tgctggccgt
     9301 cgacgggctc gatgccccga tcgcgtacta ccggaatgcg acggttcgcg ccgggcagtt
     9361 cgtggcgcac gtggagcatg tggcgcgcgc gctgcctcgc gcggcgcacg tgatcaatct
     9421 ttcggaaaac cgctatcact tcctgctcgg ctgggtggcc gcgtgcctgc gggatcaggt
     9481 gacgctgctg ccttcgggcc ggacggacga cgtgctggca ggattgcgcc accaatatcc
     9541 ggaccatcac acgatcgacg gggaatcgat tgagcgcctc ctctcgcagg cgcccacgtc
     9601 gggcagcaaa acgattccgg cgaattggca attgcctgcg gagcgcatcg tcgcaatcgc
     9661 attcacatcc ggttcgacgg gcgaccccac accgcatccg aaaacctggg gcagcctgtt
     9721 tcacaattcg cggctcgccg ccgctgaagt gctgggcggc ccgaatcgcg cgctggtttc
     9781 cacggttcca ccgcagcaca tgtacgggct ggaagcgtcg ctgctgagtg cgctcaccgc
     9841 gcaaagtgtt ctcttcgacg acaagccctt ctacccggcc gacgtgcggc gcgccctcga
     9901 agccatgacg gctccgcgcg tgctggtgac cacgcccgcg catctcaagg tgctggcgga
     9961 cgccgacatc gaactaccgc cgctggaccg cgtggtctcg gccaccgcac cgttgccggt
    10021 cgagctcgcg cgacggatcg aaaccggctg gaatacgcag ctcgtggaga tctatggctg
    10081 caccgaagcc ggcgtgatgg cgcatcgaag acctgctcaa acggaaagct ggcgcacgtt
    10141 cgccggcggg acgatggcgg ccgcatcagg cgccgccgaa tatttcgcgc cgcagctggg
    10201 gggcgccgtg ccgctgcagg acgtactcga gctgcgcggc cccacggaat tttttctgcg
    10261 cggccggacc gcggacatga tcaaggtggc gggcaaacgc gcttcgctgc aggagctcac
    10321 gcggcagatt ctgtcgttgc caggcgtgga ggacgccgtg gtgttcctgc ccgacaccga
    10381 cgcacgcccc gccgccgccg tggtggcgcc cggcctcacg gcggccgcca tactgcgcga
    10441 gctgcgtaca cgcctcgatg gcgtgttcgt tccgcggccg ctggtcatcg tcgacgaatt
    10501 gccccgcaat tccgtgggca agctgcgccg cgaagagttg cttggcctgc tggcgaaggc
    10561 gcgcgcatga acgacgccat cacgaacgaa ttcgtcgtac cggcggatca tccttcgctc
    10621 gccggacatt ttcccgggca gccggtggta ccggcggtgg tgttgctcga tgcggtgctc
    10681 gcggcgatcc gcacacgtgg ggacttcgtg ctgcgatcga ttcccgtggc caaattcctg
    10741 cagcccgtgc tgccggacga acgcgtcaac ctccgcattc acttgatctc aaccgacggc
    10801 gcgcagttgc gcgcgagttt tgcaggcatg cgcgcggcgg tgctggtgtt cgagggatcg
    10861 ttcatcgtgt cggcaagttc ggaatcatga acgactggcg cagccacaag gagcgaagca
    10921 cgccgt
//
LOCUS       DASQJS010000134        13145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_382979, whole genome shotgun sequence.
ACCESSION   DASQJS010000134 DASQJS010000000
VERSION     DASQJS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382979"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1058)
                     /locus_tag="VEW08_05570"
     CDS             complement(108..1058)
                     /locus_tag="VEW08_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HYJ40238.1"
                     /translation="MSKVLIIAEHAGGRLNASTAKCVKAATALAGAEIVVAVFAAPGS
                     PVAAQAAQIVGVTRVIDVANSLHEHALAATIAPQVAELAAPFSHIFGPSTTFGKDLMP
                     RVAALLDAPQVSDVMSIESPSRFRRPIYAGNAITTVEVSADKVVATIRIASFEAAGTG
                     GSAAVEAGAPAVALPTHTRFVSLSAAGGDRPDLQSANKVVSGGRALASSDAFKLIYSL
                     ADKLGAAVGASRAAVDAGYVPSDMQVGQTGKIIAPELYVAIGISGAIQHLTGIKDART
                     IVAINKDGEAPIFEVADIGIVGDLFTIVPELEKLIDASRK"
     gene            complement(1055..1819)
                     /locus_tag="VEW08_05575"
     CDS             complement(1055..1819)
                     /locus_tag="VEW08_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HYJ40239.1"
                     /translation="MENSQPRRILVAIKRVVDYNVRIRVKPDGTGVALDGVKLSINPF
                     DAIAIEEALRIKERGRADEVVITTIGPADCQATLRNALAMGAQRAVHVVADTPLEPLD
                     NARVLHELVKREQPFLVILGKQAIDDDNNQTGQMLAALWNRPQATFASKLELTDTSAK
                     VTREVDAGLETLEVDLPAVITTDLRLNEPRFVKLPEIMKAKTKPLASIQLSEVGGAGK
                     ARVKVQKTEPPAGRQRGIMVKDTAELFAELSKRGLV"
     gene            1859..2782
                     /locus_tag="VEW08_05580"
     CDS             1859..2782
                     /locus_tag="VEW08_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYJ40240.1"
                     /translation="MLTYTLRRIAGLVPTLFVLVSLSFFIIRLAPGGPFDQPQELPPE
                     IRANLDAAYGFDKPLISQYGNYLAGVVQGDLGPSLRYRDFTVTELITRGLPLSLTIGL
                     AAALLAFFTGVPLGAYAAWRADSMADHAIMSFSMLGVVLPGFVVGPLLALLFGIYWPI
                     FRVGGYEPGVLSFLVLPVITLALPVSAYIARLMRNSVEEVLRANYIRTARAKGLAARD
                     VFLRHALRPALIPVVSYLGPAVAFVVTGSLVVESVFGLPGSGRFLIQGAVDRDYPMVM
                     GMILVYGVLTLLCNLVADLLYAWLDPRVRLI"
     gene            2779..3588
                     /locus_tag="VEW08_05585"
     CDS             2779..3588
                     /locus_tag="VEW08_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYJ40241.1"
                     /translation="MSRRTRAALIVLCLLAALALLAPWITPYSHEYIDWERQAAPPQL
                     EAAHWLGTDRLGRDLFARSMQALRVSLLIGVLAALVSVCIGVAWGATSGYVGGRVDAL
                     MMRFVDIVYALPYVFIVIILSTMFERGSIAALFIAIGAVGWLTMARIVRGQTLSLRRR
                     EFVDAAIVSGVRTRQILWRHIVPNVIGSVIAYATLAIPQLILFESFLSFLGLGVQEPF
                     ASLGSLINEGAQEMESSPWMLLVPATLLTVLLLAFTLLGYGLRATLESRDD"
     gene            3581..5401
                     /locus_tag="VEW08_05590"
     CDS             3581..5401
                     /locus_tag="VEW08_05590"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ40242.1"
                     /translation="MTEALLTVEDLSVSYGTTAAVDSISLEILPGRCLGIIGESGAGK
                     TQAFLAMMGLLPVQARIAGRARLEGHDLLGPAAAALRGRRVAMIFQDPMTSLTPHMRV
                     GDQIAEPLVTHQGLSWREARVRAATLLEQVRMSDVPRRLRQYPHELSGGMRQRAMIAM
                     ALACDPRLLIADEPTTALDVSVQAQILALLRQLGSERGMALAVVTHDMGVIAALADDV
                     AVMREGRIVERGPVARILDAPEHAYTRALLAATPRVETPGAGISRASGHAPTTTAPSP
                     LAIHNLRVSHRLRAGWLSRPSTLSAVDGVSLRIEAGEALGIVGESGCGKSTLSRALLR
                     LGPVTAGEIVWLGRPIQDLGGEELRTLRAGMQIVFQDPYASLDPTMRVKEIVAEPLRA
                     LRPDMDAAQRASRVAAMLTSVGLGPEYASRRSRELSGGQCQRVAVARAMILEPQLLVC
                     DEAVSALDVSIQAQLLELFAAIKRDHGTSMLFVSHNLAVVRKLCERVLVMYLGRVVEE
                     GPTEEVFLRPRHPYTRMLLESVPLLDPVRERARLAVLEVQGEAPSAFDRPSGCHFHTR
                     CPAAQPMCADRSPEPEEVAKSHQVACLRWRQIATKHGQES"
     gene            5495..6769
                     /locus_tag="VEW08_05595"
     CDS             5495..6769
                     /locus_tag="VEW08_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYJ40243.1"
                     /translation="MSPENHIRPSEGLRHVKYEIRGRLARRAQELERQGYEIVPLNIG
                     NPGAFGFRTPETMRLAMIENMAVAEGYCHQKGIFPAREAVVMQQQLRGVTGVSAEEVF
                     IGNGVSELIDLCLRALLNEGDEVLIPSPDYPLWSAAVTLNGGKAIYYPCRPENDFVPD
                     PAEIASLVTRKTRAIVVINPNNPTGAVYPRAVCEGIARLAETNKLVVFSDEIYDQMTY
                     EGAESIPMATLVRDTVCCTMSGLSKIYRACGYRVGWAAFSGNLRGAGDYLSALELLSS
                     LRLCSNVPAQWAVQTALGGYQSIKELLAPGGRLFESRRAILEATSSSRFLSLAPPKGS
                     MYAFVRVKTEEILNFDDQAFALDLLEQKHVLLAPGTSFNVPYRDHFRTTLLPDAALLK
                     KVFARIDELLSAYASQSAGGAPRVVHAESRFK"
     gene            6776..9415
                     /locus_tag="VEW08_05600"
     CDS             6776..9415
                     /locus_tag="VEW08_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HYJ40244.1"
                     /translation="MDKRLPAPVFDGLNAGATLIVPSPQRQAAVRAAWVQAQRDAGLT
                     WWNTPRILTFGQFAERLLQDQWARAGLPDQLLPAGAEWACIRELRREAGGAAEARALQ
                     NSVRTLFDWRIAASPRVLGLSPESELLAEALAALERLSVEQNRKPMRAWLDELQPPSE
                     KLVAAGVANLPAAARETLLRLGVLEKSTGSAIEAAGAAPVFVAAAQNDEHELELIASW
                     CRSQLELDPGRRLLIVDSKLRQRRGLYDRLLSQTLTPSDWVAPVARSASTVFAIEGGR
                     PLAEFPLIAHALLTLRLLTGRLRFDEVVRWLRMPFLDTSDVMAGAAVEAVLREGRKLE
                     FTGEALASSLERAKGVATGALAARLRVALTTLAGPRRTPAEWSPRLLGSLRQLGWHGA
                     RALRSDEQQTVNRWHALLDEYSALGPWLTQSTASDAVATLADLARERNFDAASVEAPV
                     TITESHDDPVVHYDGIWVAGLDAAQWPPAPRPDVFIPLRLQVAAGVPWASAAAQAAAA
                     KASLAAWRSSTSQLICSWARLEGDAQRSPSPLLIHLRERKEYTPTSCVAPLAGALRRP
                     EMEVIEDELGVRVDTSRPVAGGVKPLTLQAECGFRAYGEMRLRADELETPAPGLDARE
                     RGMLLHKALELVWRKLDHFTLTVTETQVLRPTIADSVAAAVVAVFHGDIPVDLRPAVD
                     RETMRLERLIANLLDVERTRASFTVEVLEARREVGIAGGQFEFRIDRIDSIEGGGYAI
                     LDYKSGEPRAPRWNGEAVRDPQLLAYLMAERGRNVQALANVSLVNGRATFSGKSSHRG
                     LLPGVSGLPGMNPNKVPPEQIAAAWEAETGRWLHGLQMVAADYISGHAPVQPAADVCR
                     NCHLTTLCRRVELAHLDVSGEDA"
     gene            9412..12768
                     /locus_tag="VEW08_05605"
     CDS             9412..12768
                     /locus_tag="VEW08_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HYJ40245.1"
                     /translation="MSELEAADALARERALDVSDSFIVQAPAGSGKTTVLTQRYLRLL
                     TTVDEPEQVLAITFTRKAAGEMRERVQKALEGDIENRSPADALTLELAAAVRAHGAQR
                     AWGLEDSAARLRIQTIDAFNGYLANSLPITSRNGFGRTIADAPDDLYAAAARETLRDA
                     ENDPDLRKDFELILRRLDDSWQRLELLIAGMLPKRAEWLPNLPQLSGEALVPKIEASL
                     AAIVSEELAQAAASIPASFVEQASALARFAAQHVDAVELPDVASWRDNTLALTSALED
                     LARWRGIKHLTLTEKGTARARLTKNEGIPADNSLGKRLRDDWLQRLVIIDPDLLAALG
                     AIGSLPDPIVSEKDRGALEALARLLLLAATQLTRLFNVHGECDHVEIAGAARRALTEN
                     SSPTPLAERIGTRLMHILVDEFQDTSRDQYELLRTLTQDWSPGDGRTLFLVGDPMQSI
                     YGFRNAEVGRFSTVRAAGLAGLPLIPLELRRNFRSAPALVHWCNDVFARVFPTADDVR
                     KGAVRHLASVAARTNLSGEPRLYRVEGDCGRQAEADHAADLIAGLKLTRPAETIAVLA
                     GARSHLRAIRESLEKRGVPFIGVKLEPLADVPVVRDLEALVRALDSPLDRVAWLAVLR
                     APFVGLGLADLTVVSESAGEGSILSALIDGIHGLSPDGMERLLRAKPLLLAAWQERER
                     ESRAQMVERTWLGLGGASAARAGELAHARRFLAALDDEDGKRLRGRQLDLDRLMGRLY
                     AEDPALPDAVSLMTIHGAKGLEFDHVFVVGVGLRGRADDPRLLNWLEVPREQGGDHLV
                     MAPIRYRGDEEDPGDDAINLYLKQLHRERSRAERARQAYVALTRARRSLHLFVHPRVK
                     ETDGALDFGADSNSLLHSLWPAIGADAASFHALGGDADSAEDVAPAATQTRQRLRRQP
                     APLEPPADVRAQGQILPSIADQDEIEFSWARQTARRVGTVVHEALERFGRAGLPPADE
                     LPRMRARLESRLQALGIEKQAAKDGAEFALKALSATLADTRGRWLFDPGHTQAESELA
                     LSGVRGADIINAVIDRTFVDAEGTRWVVDFKTSPHEGGDRENFLDEEVKRYSAQLTRY
                     VYLARQLGPQPVRAGLYYPLLAAWREVDVG"
     gene            complement(12765..>13145)
                     /gene="gspN"
                     /locus_tag="VEW08_05610"
     CDS             complement(12765..>13145)
                     /gene="gspN"
                     /locus_tag="VEW08_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HYJ40246.1"
                     /translation="RDQRGNVVAEFKRVVLSDGPALASLEGTIELHDFRQVGANPLEL
                     GSYELTFDGSPSTNGSVVGQLRDLGGPFMVSGTVTLSSPNSYLVQGFITGRTANAERL
                     VREITLGAPPDASGRSVFSFEGSF"
BASE COUNT         2126 a         4275 c         4364 g         2380 t
ORIGIN      
        1 gtttcgcact catcgtgaag gacggcgttg cgaccaccgt cgagatcgaa gcaccgaagg
       61 agttcaaagt ctcctcggcc gaatacatcc tctcgaaact ctaacgccta ctttcgagag
      121 gcatcgatca gcttctcgag ctccggcacg atggtaaaga ggtcgccgac gattccaatg
      181 tcggcgacct cgaagatcgg cgcttcgccg tccttgttga tggcgacgat cgtgcgcgca
      241 tccttgatgc cggtgaggtg ttgaatggcg ccggagatgc cgatggccac gtacagctcc
      301 ggcgcgatga tctttccggt ctgtcccacc tgcatgtcgc tcggcacgta accggcgtcc
      361 accgcggcgc gcgaagcgcc aacggcggca ccgagcttgt ccgccagcga gtagatgagc
      421 ttgaaggcat cgctgctcgc cagcgcccgg cctcctgaca ccaccttgtt tgcgctctgc
      481 aagtccggcc ggtcgccgcc ggccgcggac aacgaaacga aacgcgtgtg tgtcggcagg
      541 gccacggcgg gcgctcccgc ttcaaccgcg gcgcttccgc ccgtacccgc cgcctcgaac
      601 gaggcaatgc gaatggtggc aacgacctta tcggcgctca cttcgacggt ggtgatggca
      661 ttgcccgcgt agataggtcg gcgaaagcgc gaggggcttt cgatggacat gacgtccgag
      721 acctggggcg catcgagcag cgcggcgact cgcggcatca ggtctttgcc gaacgtggtc
      781 gaaggcccga agatgtgtga aaacggtgct gccagctcgg cgacctgggg ggcgatggtg
      841 gcggcgagcg cgtgttcgtg cagcgagttc gccacgtcga tgacccgcgt gacgccgacg
      901 atctgcgcgg cctgtgccgc cacgggcgaa ccgggcgccg cgaatacggc cacgacgatt
      961 tccgcgccgg cgagcgcagt ggccgccttc acgcacttcg cggtggaggc attcaacctg
     1021 ccgccggcat gctcggcgat gatgagtacc ttgctcatac cagaccccgc ttggaaagct
     1081 ccgcgaacag ttcagcggtg tccttgacca tgatgccgcg ctgccggccc gcaggcggct
     1141 cggtcttctg cacttttacg cgcgccttgc cggccccgcc gacttcactc aattgaatgc
     1201 tggcgagcgg cttggtcttg gctttcatga tttccggcaa tttcacgaac cgtggctcgt
     1261 tgagtcgcag gtcggtggta atgaccgccg gcaaatccac ctcgagcgtc tcgagtccgg
     1321 cgtcgacctc gcgagtgacc ttggccgagg tgtccgtgag ctcgagtttc gacgcaaacg
     1381 tggcctgcgg gcggttccac agtgccgcca gcatctggcc cgtctggttg ttgtcgtcat
     1441 cgatggcctg tttgccgagg atgacgagga atggctgttc gcgtttgacg agttcgtgca
     1501 aaacacgcgc gttgtcgagg ggctcgagcg gcgtgtcggc caccacgtgc acggcgcgtt
     1561 gcgcacccat ggccagcgca tttcgcagtg tggcctgaca atccgcgggg ccgatggtgg
     1621 tgatcacgac ttcgtcggca cgcccgcgct ccttgatgcg caacgcctct tcgatggcaa
     1681 tggcatcgaa gggattgatg ctcaacttga caccgtcgag cgcgacgccg gtgccatcgg
     1741 gtttcacgcg gatgcgtacg ttgtagtcga cgacgcgttt gattgcgacg agaattcgcc
     1801 ggggttgtga attttccatc gtttgaagtg tagcgggagt cgtcgaggtg ttcgagggat
     1861 gttgacctac acgctgcggc gtatcgccgg cctcgttccg acgctgttcg tgctcgtctc
     1921 gttgtcgttc ttcatcatcc ggctcgcgcc cggcggaccc ttcgatcagc cgcaggagtt
     1981 gccgcccgag atccgcgcca atctcgacgc cgcatacggc ttcgacaagc cgctgatttc
     2041 gcagtacgga aattacctgg ccggagtggt gcagggcgat ctcggtccat cgctgcgcta
     2101 cagggacttc accgtcaccg agctcataac gcgcggcctg ccgttgagcc taactatcgg
     2161 gctggcggcg gccctgctgg cgttcttcac cggcgtgccg ctcggcgcat acgcggcatg
     2221 gcgcgccgac tccatggccg atcatgccat catgagtttc tccatgctgg gcgtggtgtt
     2281 gccgggattc gtggtcgggc cgttgctggc actgctgttc ggtatctact ggccgatatt
     2341 ccgggttggc ggctacgagc cgggagtgtt gagttttctc gtgttgccgg tgatcacgct
     2401 ggcgctgccg gtgtcggcct atatcgcccg cctcatgcgc aacagcgtcg aggaagtgct
     2461 gcgcgcgaac tacattcgca ccgcgcgcgc caaaggtctt gccgcccgcg atgttttcct
     2521 gcggcacgcg ttgcgtccgg ccctgattcc ggtggtcagc tacctgggtc ccgcggtagc
     2581 tttcgtggtg acgggctcat tggtggtcga atcggtgttt ggtctgcccg ggtccggccg
     2641 attcctcatc cagggcgccg tcgatcgcga ctatcccatg gtcatgggaa tgatcctggt
     2701 ctacggcgtg ctcacgctgc tgtgcaatct cgtcgcggac ctgctgtacg cctggctgga
     2761 tccgcgcgtg cgtctcatat gagccgccgg acacgcgccg cgctcatcgt gctttgcctg
     2821 ctggccgcgc tggcgctgct tgcgccgtgg ataacgcctt acagccacga atacatcgac
     2881 tgggagcggc aggcggcgcc accgcagctc gaggccgcgc attggctggg cacggaccgg
     2941 ttgggccgcg acttgtttgc acgcagcatg caggcgctgc gtgtctcgct gctgatcggc
     3001 gtgctcgcgg cgctggtcag cgtgtgtatc ggtgttgcct ggggcgccac gtcgggttac
     3061 gtcggcggcc gcgtggacgc gctcatgatg cgtttcgtcg acatcgtcta cgcgttgccg
     3121 tacgtattca tcgtcatcat cctttcgacg atgttcgaac gcggcagcat cgccgcactg
     3181 ttcatcgcca tcggcgcggt cggctggctc accatggcac gcatcgtgcg tggccagaca
     3241 ctctcgctga gacgccggga gttcgtcgat gccgccatcg tcagcggcgt gcgtacgcgg
     3301 cagatcctct ggcgacacat cgtgcccaat gtcatagggt cggtgattgc gtacgcgacg
     3361 ctagccatcc cgcaattgat cctgttcgag agtttcctga gtttcctcgg gctcggcgtt
     3421 caggagccgt ttgccagtct cggcagcctg atcaacgaag gcgcacagga aatggaatcc
     3481 tcaccctgga tgttgctggt accagccacg ctgctcacgg tgctgctgct ggccttcacc
     3541 ctgctcggct acggattgcg cgccacgctc gagtctcgcg atgactgagg cgctgctgac
     3601 cgtcgaagac ctttctgtga gttacggcac cacggcggcc gtggattcca tttcgctcga
     3661 gatcctgccg ggccgctgcc tcggcatcat cggtgaatcc ggggccggca agacccaggc
     3721 gttcctcgcc atgatggggc tgctgccggt gcaggcgcgc attgccggcc gcgcacgcct
     3781 ggaagggcac gacctgctcg gacccgccgc ggccgcgctt cgcggccgga gggtggcgat
     3841 gatcttccag gatccgatga cgtcgctcac gccacacatg cgcgttggcg accagatcgc
     3901 cgagccgctg gttacacacc agggattgag ctggcgcgag gcccgggtac gcgccgcgac
     3961 gctgctcgag caggtgcgca tgagcgacgt gcctcgccgc ctgcgccagt atccgcacga
     4021 actgtcgggt ggcatgcggc agcgcgccat gatcgccatg gcgctcgcct gcgatcccag
     4081 actgctgatc gccgacgaac cgacgaccgc actcgacgtc tcggtgcagg cgcagattct
     4141 cgcgctgctg cgccagctcg gatcagaacg cggcatggcg ctggcggtgg tgacgcatga
     4201 catgggagtc atcgccgcac ttgccgatga cgtggcggtg atgcgtgaag gccgtatcgt
     4261 cgaacgcggg ccggtggcgc gcatcctgga cgcgccggaa cacgcctaca ctcgagcctt
     4321 gttagccgca acgccgcgcg tggagacccc gggcgcgggg atctcgcgcg ccagcggtca
     4381 cgctcccacg accaccgcgc cgagcccact cgcgatacac aacctgcgcg taagccaccg
     4441 gttgcgcgcc ggctggttga gccggccgtc gacgctgtct gcggtcgatg gtgtgtcgct
     4501 gcgcatcgaa gcgggtgagg cgttgggcat cgtcggtgag tcgggttgcg gcaagtcgac
     4561 gctgagccgc gcactgctgc ggctgggacc ggtaacggcc ggcgagatcg tgtggttagg
     4621 gcggccgatc caggatctcg gcggtgaaga actcaggacc cttcgtgccg gcatgcagat
     4681 cgtcttccag gatccgtatg ccagcctcga cccgaccatg cgcgttaaag aaatcgtcgc
     4741 cgaaccgctt cgcgccctgc gaccggacat ggacgccgcg caacgcgcct cgcgtgtggc
     4801 ggccatgttg accagtgtcg gcctcgggcc tgaatacgcg agccggcgca gccgcgaact
     4861 ttccggtggc cagtgtcaac gcgtggcagt cgcgcgcgcc atgatcctcg agccgcaact
     4921 actggtgtgc gacgaagcgg tcagcgcact cgatgtgtcg attcaggcac agttgctgga
     4981 gcttttcgcg gcgatcaagc gcgaccacgg caccagcatg cttttcgtga gtcacaacct
     5041 cgccgtggtg cgcaagctct gtgagcgcgt gctcgtgatg tacctcgggc gcgtggtcga
     5101 ggaggggccc accgaggaag tgttcctgcg gccccgtcat ccgtacacgc gcatgttgtt
     5161 agagtcggtg ccgctgctcg atccggtgcg tgagcgcgcg cgcctcgcgg tgctcgaagt
     5221 gcagggagaa gcaccctcgg cgttcgatcg gccgagcgga tgccatttcc atactcgttg
     5281 tccggccgcg cagcccatgt gcgccgaccg cagtccagag ccggaagaag tcgcgaaatc
     5341 gcaccaggtg gcctgcctgc ggtggcgcca gatcgccacc aagcatggac aagaatcgtg
     5401 aaatgatccg gattccggca cgccggactg cgcaaggcgt gtgggttcgc gttattctcc
     5461 ggtgactgtt tttctccttt tctacggtag cgtcatgtcc cccgaaaacc atatccgccc
     5521 cagcgaaggc ctgcgtcacg tcaagtacga gatccgcggc cgtctcgctc gacgcgccca
     5581 ggaactcgaa cgccagggtt acgagattgt gccgttgaac atcggcaatc ccggggcttt
     5641 cggcttccgc acacccgaga ccatgcgcct cgccatgatc gaaaacatgg cggtggccga
     5701 aggttattgc catcagaagg gaatctttcc ggcgcgtgaa gccgtggtca tgcaacaaca
     5761 gttgcgcggc gtcaccggcg tcagcgccga agaggtgttc atcggcaatg gcgtgagcga
     5821 gctcatcgat ctttgcctgc gtgcgctcct caacgagggc gacgaggtgc tgattccgag
     5881 ccctgactac ccgctgtggt ccgcggcggt cacgctgaat ggcggcaagg cgatttacta
     5941 tccgtgccgc cccgagaatg acttcgtgcc tgacccggcc gagatcgcct cgctggtgac
     6001 gcgcaagacg cgcgccatcg tcgtcatcaa tcctaacaac ccgaccggcg cggtctatcc
     6061 acgcgcggtg tgcgaaggca tcgcgcgcct ggcggaaacg aacaagctcg tggtcttcag
     6121 cgacgagatt tacgaccaga tgacctatga aggcgccgag tccattccga tggcgacgct
     6181 ggtgcgcgat accgtgtgct gcacgatgtc cggcctgtcg aaaatctacc gcgcctgcgg
     6241 ctatcgggtc ggctgggcgg cgttctccgg caacctgcgc ggcgccggcg actacctttc
     6301 cgcgctggag cttctctcgt cgttgcggct gtgctcgaac gtgccggcgc aatgggcggt
     6361 gcagaccgcg ctcggcggct accagagcat caaggaactg ctcgcacccg gcggccggtt
     6421 gttcgaatcc cgtcgcgcca ttctcgaagc cacttcctcg agccgctttc tctccctggc
     6481 gccgcccaag ggatccatgt acgcgttcgt gcgcgtaaag acggaagaga tactcaattt
     6541 cgacgaccag gcgttcgctc tcgatctgct ggagcagaaa cacgttctgc tcgccccggg
     6601 caccagtttc aacgttccgt atcgcgatca tttccgcacc acactgctgc cggatgcggc
     6661 gctgctcaag aaagtgttcg cccgcatcga cgaactgctt tccgcgtacg ccagccagag
     6721 tgccggcgga gcgccgcgtg tcgtccacgc ggaatcgcgt ttcaagtaac agttattgga
     6781 caagcgactt ccggcccctg ttttcgatgg cctgaacgcg ggcgcgacgc tcatcgtgcc
     6841 aagcccgcag cgccaggccg cggttcgcgc ggcctgggta caagctcagc gtgacgccgg
     6901 cctgacgtgg tggaacacac cacgcatcct gacgtttggc cagttcgcgg agcggttgct
     6961 ccaggaccaa tgggcgcgcg ccggtctccc ggatcaattg ctgccggcgg gtgcagagtg
     7021 ggcttgcatc cgtgaattac gccgcgaggc gggtggcgcc gccgaggcgc gcgcgttgca
     7081 gaattccgtg cggacacttt tcgattggcg catcgccgct tcgccgcgcg tgctcggact
     7141 ctcgccggaa tcagagctgc tggccgaagc tctggctgcg ctcgagcgcc tgtccgtcga
     7201 acagaatcgc aagcccatgc gcgcctggct cgacgaacta cagccgccgt cggaaaaatt
     7261 ggtggccgcg ggtgtagcca accttccggc cgctgcgcgg gagaccctgc ttcgcctggg
     7321 cgtgctggaa aaatctaccg gttctgcaat tgaagcggcg ggcgccgcgc cggtcttcgt
     7381 cgctgccgct cagaacgatg agcacgagct cgaattgatc gcttcgtggt gccgcagcca
     7441 gctcgaactg gatcccggtc ggcggctgct catcgtcgac tcgaagcttc gccagcgtcg
     7501 cgggctctac gatcgcctgc tgtcgcagac gctgacgccc tctgactggg tcgcgcctgt
     7561 ggcgcgcagc gcttcaaccg tgtttgcgat cgaaggtggc cggccgctgg ccgagttccc
     7621 actcatcgcc cacgcattgc tgaccttgcg cctgctgacg gggcgcttgc gattcgacga
     7681 agtcgtgcgc tggttgcgaa tgccgtttct ggacacgagc gacgtcatgg ccggcgccgc
     7741 cgtcgaagcg gtactgcgcg aaggaaggaa actcgaattc acgggtgagg cgcttgcgag
     7801 ctcgctcgaa cgcgcgaagg gcgtcgcaac cggcgctctc gcggcgcgcc tccgcgtggc
     7861 gttgacgacg ctggcagggc cgcgccgtac ccccgccgag tggtcaccgc gcctgctcgg
     7921 atcacttcga cagttgggct ggcacggcgc ccgtgcgctg cgcagcgacg agcagcagac
     7981 cgtgaaccgc tggcacgcgt tgctcgacga atattcggcg ctcggaccct ggctcacgca
     8041 atcgaccgcg tccgacgccg tggcaacgct ggccgacctg gctcgagagc ggaacttcga
     8101 cgcggccagc gtcgaagcac cggtgacgat caccgaatcg cacgacgatc cggtggtgca
     8161 ttacgacggc atctgggtgg ctgggctcga tgccgcgcag tggccgccgg cgccacggcc
     8221 cgacgtcttc ataccgctac gcctgcaagt ggcggccgga gtcccatggg ccagcgccgc
     8281 cgcacaggcc gccgccgcga aggcctcgct cgcggcatgg cgttcgagca cgagccaact
     8341 gatttgctcc tgggcgcgtc tcgaaggtga cgcgcaacgc tcaccgagcc cgctgctgat
     8401 tcacttgcgc gaacgcaagg agtacacgcc gacatcgtgc gtcgccccgc tggcgggcgc
     8461 cttgcgccgc cccgagatgg aagtgatcga agacgagctg ggcgtgcgag tggataccag
     8521 ccggccggtg gccggcggcg tcaagccgct cacgttgcag gccgagtgtg gcttccgcgc
     8581 gtacggcgag atgcgtctcc gcgccgacga gctcgagacg cccgcacccg gcctcgatgc
     8641 gcgcgagcgc ggcatgctgc tgcacaaggc gctcgaactc gtgtggagaa agctcgatca
     8701 tttcacgttg accgtcaccg agacgcaggt gttgcgcccg accatcgcgg actcggtcgc
     8761 ggccgccgtg gttgctgttt tccacggcga cattccggtg gatttgcgtc cggcggtcga
     8821 ccgcgaaacg atgcggctcg agaggctcat cgcaaacctg ctcgacgtcg agcgcacgcg
     8881 cgcatcgttt acggttgaag tactcgaggc gcgccgcgag gtcggcatcg ccggcgggca
     8941 gttcgaattc cgcatcgacc gcatcgattc catcgaaggc gggggttacg ccatcctcga
     9001 ctacaagtcg ggcgaaccgc gcgcgccgcg ctggaacggc gaagcggtac gtgatccgca
     9061 gttgctggcc tacctcatgg cagaacgcgg tcgcaatgtg caggcgctgg ccaatgtatc
     9121 tctcgtcaat ggccgcgcga cgttcagcgg caagagctcg caccgggggc tgcttccggg
     9181 cgtcagcgga ttgcccggca tgaaccctaa caaggtgccg ccggagcaga tcgccgctgc
     9241 ctgggaggcc gagacggggc gctggctgca cgggttgcag atggtggcgg ccgactacat
     9301 ctcgggccat gcgccggtgc agccggccgc ggatgtctgc cgcaattgcc acctcacgac
     9361 gctctgccgg cgcgtcgagc tcgcgcacct cgacgtcagc ggagaggacg catgagcgaa
     9421 ctcgaagctg ccgatgcgct tgcgcgcgaa cgcgcgctgg atgtctcgga ctcgttcatc
     9481 gtccaggcgc ctgcgggttc cggcaagacc acggtgctca cacagcgata tctccggttg
     9541 ctgacgacgg tggatgagcc cgagcaggtg ctcgccatca cctttacgcg caaggcggct
     9601 ggcgaaatgc gagagcgcgt ccagaaggcg ctcgaaggcg atatcgaaaa ccgctcaccg
     9661 gccgatgctc tcacgctcga actcgcggca gcggtgcgtg cgcatggcgc gcaacgtgcg
     9721 tggggtctcg aagacagcgc ggcgcgcctg cgcatccaga ccatcgacgc gttcaacgga
     9781 tacctcgcca actcgctgcc catcacttcc cggaatggct tcggtcgcac catcgccgat
     9841 gcgcccgacg acctgtacgc cgcggccgcg cgcgagactc ttcgcgacgc cgagaacgat
     9901 cccgatctgc gcaaggattt cgagctcatc ctgcgccggc tcgatgacag ctggcagcgc
     9961 ctggaattgc tgattgccgg gatgctcccg aagcgcgccg aatggctgcc caacctgccg
    10021 cagctttcgg gcgaggcgct ggtaccgaaa atcgaggcga gcctcgccgc catcgtgagc
    10081 gaagagctcg cgcaggctgc cgcctcgata cccgcgtcgt tcgtcgagca ggccagcgca
    10141 ctcgcacggt ttgccgcgca gcatgtcgac gccgtcgaac taccggatgt cgccagttgg
    10201 cgtgataaca cgctggcgct cacgagcgct cttgaagacc tcgcgcggtg gcgcggcatc
    10261 aagcatctca cgttgaccga gaagggcacc gcgcgcgcgc gtctcaccaa gaacgagggc
    10321 attccggccg acaattcact gggcaagagg ctgcgtgatg attggctgca gcggctggtg
    10381 atcatcgacc ccgacctgct ggccgcgctg ggcgccattg gttccttgcc cgatccgatc
    10441 gtgtccgaga aggatcgcgg agctctcgaa gcgctcgcca ggctgctact gctcgccgcg
    10501 acgcagctca cgcgtctgtt caacgtgcat ggcgagtgcg atcacgtcga gatcgccggc
    10561 gccgcgcgcc gcgcgctcac cgagaacagc agcccgacgc cgctcgcaga gcgtattggc
    10621 acgcggctga tgcacatcct cgtcgacgag ttccaggaca cttcgcgcga ccaatacgag
    10681 cttctgcgga cgctcacgca ggattggtct ccgggcgatg ggcgcaccct gttcctggtc
    10741 ggcgacccga tgcaatccat ctacggcttt cgaaacgcgg aggtaggccg cttctcgacc
    10801 gtgcgcgctg ccggcctcgc gggattaccc cttatcccgc tcgaacttcg ccgcaacttc
    10861 cgctcggcgc cggcgctggt gcactggtgc aacgacgtgt tcgcgagggt atttcccacc
    10921 gccgatgacg tgcgcaaggg cgcggtgcgt cacctggcga gcgtcgcagc gcggaccaat
    10981 ctgagcggcg agccgcgtct ctatcgcgtg gagggagatt gcggcaggca ggccgaggcc
    11041 gaccacgccg cggatctcat cgccgggttg aagctcacgc gaccagccga aaccattgca
    11101 gtgctcgcgg gcgcgcgttc acacttgcgc gcgatccgcg agagtctgga aaagcgtggg
    11161 gtgcccttca ttggcgtgaa gctcgagccg ctggcagatg tcccggtggt tcgagacctc
    11221 gaagcgctgg tgcgcgccct cgactcgccc ctcgaccggg tggcgtggtt ggcggtgctt
    11281 cgcgccccat tcgtcggact gggcctcgcc gatctcacgg tggtcagcga atccgctggc
    11341 gaaggctcga tactgtcggc cttgatcgat gggatccatg ggctgtcgcc ggatgggatg
    11401 gaacggctgc tgcgtgcaaa gccgctattg ctggcggcgt ggcaggagcg cgaacgcgaa
    11461 tctcgagctc aaatggtcga gcggacctgg ctggggctcg gaggcgcgtc ggccgcgcgc
    11521 gcgggtgaac tcgcgcatgc gcggcggttc ctcgcggcgc tcgacgatga agatggcaag
    11581 cgacttcgcg gccgccagct ggatctcgac cggctcatgg gacgtttgta cgcggaagac
    11641 ccggcgctac ccgacgccgt ctcgctcatg accatccacg gcgccaaggg gctggaattc
    11701 gatcatgtat tcgtggtcgg cgtggggttg cgaggacgcg ccgatgatcc gcgtctgttg
    11761 aactggctcg aggttccacg cgagcagggt ggcgatcacc tggtgatggc gccgattcgt
    11821 tatcgaggcg acgaggaaga cccgggagac gacgccatca atctctacct gaagcagttg
    11881 catcgcgaac ggtcccgcgc ggagcgcgcg cgacaggcgt acgtcgcgct gacgcgagcc
    11941 aggcgttcgc tgcacctgtt cgttcatccg cgcgtgaagg aaaccgacgg cgccctggac
    12001 ttcggcgcag attcgaactc cctgttgcac agcctctggc ccgcgatcgg agcagatgcc
    12061 gcgagctttc acgcgctggg cggcgatgcc gattcagctg aagacgtcgc gccagctgcg
    12121 actcagacgc gccaacgcct gcgccggcaa cctgcgccgc tcgagccacc cgccgacgta
    12181 cgggcacaag gccagatcct gccgtcgatc gccgatcagg acgaaatcga attcagctgg
    12241 gcgcgccaga ccgcacgccg cgtcggcacc gtggtgcacg aagcgttgga gagattcggg
    12301 cgcgcggggc taccgccggc ggacgagctg ccgcgcatgc gcgcgcgcct cgagtcgcgg
    12361 ctgcaggcat tgggcatcga aaagcaggcg gccaaggatg gcgccgaatt cgccctgaag
    12421 gctttgagtg cgacgctggc cgatacgcgt ggccgctggt tgttcgatcc tggccacacg
    12481 caagccgagt cggagctggc gctctcgggc gtacggggcg ccgacatcat caacgcagtg
    12541 atcgatcgca cgtttgtcga cgccgaaggc acgcgctggg tggtggattt caagaccagt
    12601 ccgcacgagg gcggcgatcg ggagaacttc ctcgatgagg aagtgaaacg atactcggct
    12661 cagctgacgc ggtacgtgta tctggcacgc cagctcggac cgcagccggt gcgggccggc
    12721 ttgtactatc cgctgctcgc ggcgtggcgc gaagtcgacg tcggttagaa ggatccttcg
    12781 aaactgaaga cgctgcgccc ggaggcgtcg ggtggcgcac cgagtgtgat ctcgcgcacc
    12841 agccgctcgg cgtttgccgt gcgcccggtg atgaacccct gcacgaggta cgagttcggc
    12901 gaggacagcg tgaccgttcc actcaccatg aatgggccgc cgagatcgcg cagctggccc
    12961 accacgctgc cgtttgtcga tggcgacccg tcgaacgtca gctcatacga acccagctcg
    13021 agagggtttg cacccacctg gcggaaatcg tgcaactcga tggttccctc cagcgatgcg
    13081 agggcggggc cgtcggacaa caccacgcgc ttgaactcgg cgacgacgtt gccgcgctga
    13141 tcgcg
//
LOCUS       DASQJS010000135         6465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3522788, whole genome shotgun sequence.
ACCESSION   DASQJS010000135 DASQJS010000000
VERSION     DASQJS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3522788"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1561)
                     /locus_tag="VEW08_05615"
     CDS             complement(26..1561)
                     /locus_tag="VEW08_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40247.1"
                     /translation="MRKFRNPLAAAVSSACLGLFANAATGADAETAITIYSSAQPGAI
                     SPEFYRPIPGQGVPNAMAVPGYALVRDDREMKIKQGRSQLSFTDVAALIDPTTVTFTS
                     LTDPATRVLEQNFQFDLVSTQKLLLKFIDKQITVDKPNGSSVTPITGTLLSASDGIVL
                     RGNDGSIYSLPAYNSVRFPDLPGGLNTRPTLVWDIQSPAGGTHKARVTYQTGGITWWA
                     DYNLIFNEGADANSGLLDLSAWVSIINQSGATYSDAKLKLIAGDVNRVQPEVDTRMRR
                     EVLAMSAKVADEVAGFAQKDFFEFHLYTLGRKTTLPNNSTKQIELFDQAKQIPAKKVL
                     LYYGAVQPYFYPSPYTDRNMGVEMNKKVDVYLEFKNDKQFGLGVPLPAGRLRVSQLDK
                     ADDSLEFIGEDTIDHTPKDEQVRVKLGSAFDVVGERRQVNFTVDTAGKVMEEEIEVKL
                     RNHKAQPVDVVVKENLYRWSNWKILTQTHKFDQEDSRTISFPVKVAKDGETIVRYRVR
                     YTW"
     gene            1864..2640
                     /locus_tag="VEW08_05620"
     CDS             1864..2640
                     /locus_tag="VEW08_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40248.1"
                     /translation="MEAASRIESSGVNQAPAAGGTAPGTPAPAMDREFIIKNQIVERY
                     LAGRLPPKGVTDFERLIRSRPQLIEELGLADKVNAGLRLLDAAGIAEPWAEKEKKAWE
                     KPAFAMGAAALAIAALIGCAVLGMQVSSRNAIIAKQKLENLERPIAPSTRKRTILVGV
                     ARNGPPAQATLSISHGEFAELKADLSWARYASYKVSIDRVDQGRVAVLDGLEKDSNGH
                     VRISLNAGAFGPGDYMVSIEGLNMRRESIPIAWYRVAVTR"
     gene            complement(2658..4289)
                     /locus_tag="VEW08_05625"
     CDS             complement(2658..4289)
                     /locus_tag="VEW08_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40249.1"
                     /translation="MAEGKPVKRRSMVGWYDPRVLAHSAYKLVIANIFGRHSDTRLIE
                     ALASQPQNEFDYSAAQGDFWFDYVADIGDGWNSTYAIADAIARPELEGTEAGRILIFG
                     GDEVYPYPTRAEYDVRTETPYSMAFAGRVHPDVFAIPGNHDWYDSLVAFSRIFCRPER
                     GFAGCATRQTRSYFALKLPANWWLLAIDLQLGADLDEPQVQYFQKVASRMDDAARLIF
                     CVPEPRWILEDAYPSHSSYEELSSTRFLEEKVFKRKARIFLTGDLHFYKRHENAEGIQ
                     KITSGGGGAFLHPTHAPSTFQLSNGFVQRAAYPDEKTSRRLSWRNFLFPALNPKSGWL
                     YAFLYAMSAWLASASLEAADVIDAQTALAAALNAAIRDPLNGMWLVSVIGGFIFFTDT
                     HVRSWRILGGACHALLHLAAAFAVGWLALLLTVNAFDLAYGSIAQLLFSGLVTFIVGG
                     PVGAFILGVYLFVSIRVFGRHSNEAFSSLRIQDFKQWLRLRIDAAGGLTIHAIAIDRV
                     PRRWRAAQRNGKPTIEAHDARATAPRLIDKVEIRP"
     gene            complement(4289..>6465)
                     /locus_tag="VEW08_05630"
     CDS             complement(4289..>6465)
                     /locus_tag="VEW08_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYJ40250.1"
                     /translation="AVSEKYPLRGRVVLSDEPFGAPQPANGIPARGEVWPDSRLAAAL
                     GAGVGTELNIGNSTFRISRILITRPDQGATSLELAPSLIMNEDDLAATQLIQPGSRLR
                     RAQLFAGPRDDIASFKTWLEANKKQNEEIEDVEDSAPQIKSAIDRSARFLSIASLMAV
                     LLCAIAVAMAARRYVHRHLDSVALLKTLGATRAFTLTVSMVQLTMVGLLAAILGSLVG
                     FGAQAWLVKALAGLLRGDLPAPGLAPLGIGFLTAIAVLGGFALPPLLQLSRTPALRVL
                     RRDVGPPQPLIVLAFGPAVIAIAFLIYWVLRDQRTAIAFVVGLSVFIVVVALAGWLLV
                     KLTTRFRGGVGISWRYGIANLGRRRTESVVQLTAFALGIMMLLLLAVVRNDLLNDWRR
                     SLPADTPNFFFINIPPDEHQAFTTFLEERGAQHARARPMVRARMTHINGTAVDDIKFA
                     TRRGREFANRDQNITWSETLGNDNVVKAGRWFTPEDFGKPLVSVSTEYMEEMKLKLGD
                     ELQFDIAGETRAARISSVRDVKWDSFQPNFFLMFAPGLLDGSQGTWMTAAHLSTTNPK
                     TISDLVRRFPSVSVFNVDDLLRQVRAVIDKAVAAVQSVFLFTLMAGLVVLVAAVQASR
                     EERRYESAILRTLGAQRSTVLKGLLAEFAALGVLSGTLAAAGASIAGVYIATQVLQIP
                     YTADPRVWFYGLVGGGLLVCFAGWLATRSVVNQPPVLTLRGG"
BASE COUNT         1215 a         2022 c         2068 g         1160 t
ORIGIN      
        1 tgggtgcccg gcaccgcctc tttaactacc aggtatagcg cacgcgatac cggacgatgg
       61 tctctccgtc cttggccacc ttcacgggga agctgatggt gcgggaatct tcctggtcga
      121 atttgtgcgt ctgcgtcagg attttccagt tgctccagcg atagagattt tccttcacca
      181 cgacgtccac cggctgcgcc ttgtgattgc gcagcttcac ttcgatttcc tcttccatca
      241 ccttgcccgc ggtgtcgaca gtgaagttca cctggcggcg ctcacccacg acgtcgaatg
      301 cagacccgag tttcacgcgc acctgctcgt ccttcggcgt gtgatcgatc gtgtcttcgc
      361 cgatgaactc gaggctgtcg tcggccttgt cgagctgcga cacgcgcagg cggcccgcgg
      421 gcagcggcac gccgagcccg aactgcttgt cgttcttgaa ctccaggtac acgtcgacct
      481 tcttgttcat ctccacgccc atgttccggt cggtgtaagg gcttggataa aagtacggct
      541 gcaccgcgcc gtaatagaga agcaccttct tcgccgggat ctgctttgcc tggtcgaaga
      601 gctctatctg cttggtggaa ttgttcggca gcgtggtctt gcggccgagc gtgtacaggt
      661 ggaactcgaa gaagtccttc tgcgcaaagc cagccacttc gtccgccact ttcgccgaca
      721 tcgccaggac ctcgcgccgc atgcgcgtgt ccacttccgg ctgcacgcgg ttcacgtcac
      781 ccgcgatgag cttgagcttc gcgtccgaat acgtggcccc ggactggttg atgatgctga
      841 cccacgccga aagatcgagc agcccgctgt tggcatccgc gccttcgttg aagatgaggt
      901 tgtagtcggc ccaccaggtg atgccgcccg tctggtaggt gacgcgcgcc ttgtgagtgc
      961 caccggccgg cgactgaata tcccacacca gcgtcgggcg tgtgttgagg ccgccgggca
     1021 gatccgggaa tcgcaccgag ttgtacgccg gcagcgagta gatagagccg tcattgccgc
     1081 gcaacacgat accgtcgctg gccgacagca gcgtgcccgt gatcggcgtc accgagctgc
     1141 cgtttggctt gtccactgtg atctgcttgt cgatgaactt gagcagcagc ttctgcgtgc
     1201 tcaccaggtc gaactggaaa ttctgctcga gcacgcgcgt ggcagggtcc gtcagcgatg
     1261 tgaaggtgac cgtcgtcgga tcgatgagcg cggccacatc cgtgaacgac aattgagaac
     1321 gaccctgctt gatcttcatc tcgcggtcat cacgcaccag cgcatagccg ggaacagcca
     1381 tggcgttggg tacgccttgc cccggaatcg gccgatagaa ttccggtgaa atcgcaccgg
     1441 gctgcgcgga gctgtaaatc gtgatggcgg tttccgcatc ggcacctgtt gccgcattgg
     1501 cgaagaggcc gaggcaggcg gacgacacgg cggcggcgag cggattacgg aatttgcgca
     1561 tgaaaggcga acctcgttgg gatggacatg gacactaacg gttgcggtgc gatcggcggg
     1621 ctaggcccat caccggtggg aaactgcgaa ccacgtcgca agattgtcgg gaacccattt
     1681 gtggaatgtg aagcggcgca gcggcttctg gacataagaa tcgcagcata tgcaggtgcg
     1741 atcctgtagg accaacggca gataccaacc cgagtggcgc ctggctttct tgcggcgctt
     1801 acgcatcggt ttcggaagcc gtgaagtccg cccgtccctg aatctgcgga attttgaacc
     1861 acgatggaag ctgcgagtcg tatcgaatcc tctggcgtaa accaggcacc tgccgccggc
     1921 ggcactgcgc ccggcacacc tgcgcctgcg atggatcgtg aattcatcat caagaaccag
     1981 atcgtcgagc gttacctcgc cggtcgtctg ccgcccaagg gcgtcaccga ctttgaaaga
     2041 ctgattcgct cgcgtccgca actcatcgaa gagctcgggc tcgccgacaa ggtgaacgcg
     2101 ggcttgcgtc tgttagacgc ggccggcatt gccgaacctt gggccgagaa agagaaaaag
     2161 gcatgggaga agccagcctt cgcgatgggc gctgcggcgt tggccattgc cgcactgatt
     2221 ggctgcgcgg tgcttggcat gcaggtgtcc tcgcgcaacg cgatcatcgc caagcagaaa
     2281 ctggaaaatc tcgagcgtcc gattgcgcca tccacgcgca agcgaaccat tctcgtcggc
     2341 gtggcacgca atggcccgcc cgcgcaggcg accctgtcca tcagccacgg tgagttcgcc
     2401 gaattgaagg cggacctgtc gtgggcgcgt tatgcctcgt acaaggtgtc gatcgatcgc
     2461 gtggaccagg gccgcgtcgc cgttctcgac ggcctggaga aggactccaa cgggcacgtg
     2521 cgaatttcgt tgaatgcggg cgccttcggc cctggcgact acatggtgtc catcgaaggg
     2581 ctcaacatgc gtcgcgagag cattccgatt gcctggtacc gggtcgccgt cacccgctga
     2641 agacccctcg tccctgatca ggggcggatt tcaaccttat cgatgagccg cggtgcggtt
     2701 gcacgcgcgt cgtgcgcctc gatcgtcggc tttccgttgc gttgcgccgc gcgccagcgc
     2761 cgcggaaccc gatcgatggc aatcgcatgg atcgttagac cgccggccgc gtcgatgcgc
     2821 agccgcagcc actgcttgaa gtcctggatg cgcagcgacg agaacgcttc attgctgtgg
     2881 cgcccgaaga cgcgtatcga cacgaataga tagaccccca ggatgaacgc acccaccggc
     2941 ccgcccacga tgaaagtgac gagaccggaa aacagcaatt gcgcgatgct gccgtaggcg
     3001 agatcgaagg cgttcaccgt cagcagcagc gcgagccacc cgactgcgaa cgccgccgcc
     3061 agatgcagca gcgcgtgaca ggcgccgccg aggatgcgcc acgagcggac atgtgtgtcg
     3121 gtgaagaaga tgaatccgcc gatgacactg accagccaca tgccgttcag cgggtcgcgg
     3181 attgccgcgt tcaatgcagc ggcgagcgcg gtctgcgcat cgatgacgtc cgcagcctcg
     3241 aggctggccg aggcaagcca ggctgacatg gcgtagagga acgcgtacag ccagccggac
     3301 ttcgggttga gggctgggaa caggaaattc cgccatgaga gccgtctcga tgtcttctca
     3361 tcggggtagg ccgcccgctg cacgaaacca ttgctcaact gaaatgtcga cggcgcatgc
     3421 gtgggatgca ggaatgcgcc acccccgccc gaggttatct tctggattcc ttccgcgttc
     3481 tcgtgccgct tgtagaagtg cagatcgccg gtcagaaaaa tgcgcgcctt gcgcttgaac
     3541 accttttcct cgaggaatcg cgtgctcgag agctcttcgt agctcgagtg gctgggatag
     3601 gcatcctcga gaatccagcg cggctccggc acgcagaaga tcaggcgggc ggcgtcgtcc
     3661 atgcgcgagg cgactttctg gaagtactgc acctgcggct catcgagatc ggcgccgagc
     3721 tgaaggtcga ttgccagcag ccaccagttg gcgggtagtt tgagcgcgaa gtagctgcgc
     3781 gtttgccgcg tggcgcagcc ggcgaagccg cgctcgggac ggcagaagat gcgcgaaaac
     3841 gccaccaggc tgtcgtacca gtcgtggttg ccgggaatcg caaacacgtc ggggtgcacg
     3901 cggccggcaa acgccatgct gtagggggtt tcggtgcgaa cgtcatattc cgcgcgagtc
     3961 ggatagggat agacctcgtc cccaccgaaa atcaggatgc ggccggcttc ggttccttcc
     4021 agctcgggcc gtgcgatggc gtccgcgatg gcgtacgtgg aattccaccc gtcgccgatg
     4081 tccgccacgt aatcgaacca gaagtcgccc tgggcggccg agtaatcgaa ttcgttctgc
     4141 ggctggctcg cgagcgcttc gatgaggcga gtgtccgaat ggcggccgaa aatgttggct
     4201 atgaccagct tgtatgccga gtgcgcgagc acgcgcgggt cgtaccaacc caccatcgac
     4261 cggcgcttga ccggtttgcc ttcagccatc acccaccccg caaggtcagc acgggcggct
     4321 ggttcactac ggaccgcgtg gccagccagc cggcgaagca gactaacaag ccgccgccca
     4381 ccaggccgta gaaccacacc cgcggatcgg cggtataggg aatctgcagc acctgcgtcg
     4441 cgatatatac acccgcgatc gacgcccctg cggcggcgag cgtgcccgac agcacaccca
     4501 gtgccgcgaa ttccgccagc aggcccttga gcaccgtgga tcgctgtgcg ccgagtgttc
     4561 gcaggatcgc gctttcgtag cggcgttcct cacgactggc ctgcaccgcc gcgaccagca
     4621 ccaccagccc ggccatgagc gtgaagagga agacgctctg gacggcggcc acggccttgt
     4681 cgatgacggc gcgcacctgg cgcagcagat cgtcgacatt gaacacagac acgctcggga
     4741 agcggcgcac caggtccgaa atggttttcg gattggtggt ggagaggtgt gccgcggtca
     4801 tccaggttcc ctgcgaacca tcgagcagac ccggcgcgaa catcaggaag aagttcggct
     4861 ggaagctgtc ccacttcaca tcgcgcacgc tggatatcct ggcggcgcgc gtttcaccgg
     4921 cgatgtcgaa ctgcagctcg tccccgagct tgagcttcat ttcttccatg tactcggtgg
     4981 aaacggaaac cagcggcttg ccgaaatcct cgggcgtgaa ccagcggccg gccttgacca
     5041 cgttatcgtt gccaagcgtt tccgaccacg tgatgttctg atcgcggttg gcgaattcgc
     5101 ggccgcggcg cgtcgcgaac ttgatgtcgt ccacggccgt gccgttgatg tgggtcatgc
     5161 gtgcgcggac catcggccgg gcccgcgcgt gctgcgcacc tcgctcttcg agaaaggtgg
     5221 tgaacgcctg gtgttcgtcg ggcggaatgt tgatgaagaa gaaattgggc gtgtcggccg
     5281 gcaggctgcg acgccagtca ttgagcagat cgttgcggac caccgcgagc agcagcagca
     5341 tcatgatgcc gagcgcgaac gccgtgagct gcacgacgct ttcggtgcgc cgcctgccca
     5401 ggttggcgat gccatatcgc caggagatcc ccactccacc ccggaagcgc gtcgtgagtt
     5461 tgacgagcag ccaaccggcc agggcaacca cgacgatgaa tacggagagg ccgacgacga
     5521 aggcgattgc cgtacgctga tcgcgcagca cccagtagat gaggaatgcg atcgcaatca
     5581 ccgccggacc gaatgccagc acgatcagtg gctgtggagg cccgacgtcg cgacgcagga
     5641 cgcgcaaggc gggcgtgcgc gacaactgca gcagcggtgg cagcgcgaat ccgcccagca
     5701 cggcgatcgc ggtgagaaac ccgatgccaa gcggtgcaag gccgggcgcc ggcagatcgc
     5761 cgcgcagcag ccccgcgagc gcctttacga gccaagcctg ggcgccgaag cccaccagcg
     5821 aacccagaat cgcggctaac aaccccacca tcgtgagctg caccatgctc accgtcagcg
     5881 tgaacgcgcg cgttgccccc agcgtcttga gcagcgcgac cgaatcgagg tgccggtgca
     5941 catagcgccg ggccgccatg gccaccgcga tcgcgcacag caggacggcc atgagactcg
     6001 cgatgctcag gaaccgcgct gaacgatcga tcgccgactt gatctgcggg gccgagtcct
     6061 cgacgtcctc gatctcttcg ttctgcttct tgttggcctc gagccaggtc ttgaacgagg
     6121 cgatgtcgtc gcgcggaccc gcgaacaact gggcgcgccg cagacggctg ccgggttgaa
     6181 tgagctgcgt ggccgccaga tcgtcctcgt tcatgatcag gctcggagcc agctcgaggg
     6241 aggtggctcc ctggtcggga cgcgtaatga gaatgcgcga gatgcggaac gtgctattgc
     6301 cgatgttgag ctcggttccc acgcccgcgc cgagcgccgc cgcgaggcgc gaatccggcc
     6361 atacctcgcc gcgtgccgga atcccgtttg ccggctgggg cgcgccaaag ggttcatcgg
     6421 aaagcactac ccggccacga agcggatatt tttccgacac ggctc
//
LOCUS       DASQJS010000136         3342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2583165, whole genome shotgun sequence.
ACCESSION   DASQJS010000136 DASQJS010000000
VERSION     DASQJS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2583165"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="VEW08_05635"
     CDS             <1..1452
                     /locus_tag="VEW08_05635"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HYJ40251.1"
                     /translation="LPIEIATFTRSLAAAEELARVEGRPPCQVDSTDAPFNGWIRRAG
                     ADIGMMMTPTSQGRIPAAGLPWLDAIFGRDAIITALETLWLWPEIARNVLRYLAATQC
                     TSSSSGSGAEIGKILNESRAGFPTDYYGVDTTPLFVMLAGAYYTRTADLDLMTELWPA
                     ITAALRWIDVYGDVDGDGFVEARPRGTGPAQHSWKSSPDAVFHADGRLATGPLAMCEV
                     QGYVYAARLGAARIARSLGEAPRAISLVESAQALQARFNTAFWCGDIEGYALALDGEK
                     QPCRVRASNPGHCLFTGIAHSERARQVIAGLGDEHFFSGWGVRSVGESELRYNPMSYH
                     NGSVWPHDNALIAAGAARYDDKTFALRILNAQFEAARHFELMRLPELFCGFRRKGQEA
                     PLHFPVACSPHSVSAGATFLLLQSVLGISIDALDRQIVLAHPVVPDGFDAICVKNLEV
                     QDASVDFTVRRHGGSVSVSVERREGKVDLVIRS"
     gene            complement(1771..2685)
                     /locus_tag="VEW08_05640"
     CDS             complement(1771..2685)
                     /locus_tag="VEW08_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40252.1"
                     /translation="MEISARGRAATARRRTPADAKAKVANGNGGGGPAAAPDLDLKKL
                     LRALQAVRDGDFSVRLAPDQTGLGGKIADAFNEMVSANERMASELERAGQMVGKEGKT
                     RTRVSAERRSGSWGAMESSVNTLIDDLLWPTAEVTRTITAVAKGDLTQSMRLEADGRP
                     LKGEFLRSANVVNEMITQMGVFTSEVNTPICVIISLTTFADLRNSPFNGRPSASSRML
                     CVRSPLATAVIVRVTSAVGHSRSSMSVLTDDSISLQAPVRRSLDTRTRVLPSLPTIWP
                     ARSSSVAMRSFALTISLNASAILPPSPV"
     gene            complement(2818..>3342)
                     /locus_tag="VEW08_05645"
     CDS             complement(2818..>3342)
                     /locus_tag="VEW08_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40253.1"
                     /translation="EHFFSGWGVRTVAESELRYNPMSYHNGSVWPHDNALIAAGAARY
                     DDKAFALRILNSQYEAARHFDLLRLPELFCGFKRRGREAPLQFPVACTPQAASAGATF
                     LLLQSVLGIGIDALDRQIVLAHPVVPDGFDAICVKNLQVQDASVDFTVRRHTGSVSVS
                     VERREGKVDLVIRS"
BASE COUNT          571 a         1078 c         1098 g          595 t
ORIGIN      
        1 cttccgatcg agatcgccac cttcacgcgg tcgctggccg cggccgaaga attggcgcgc
       61 gtcgagggcc ggccgccgtg ccaggtggat tcgaccgacg cgccgttcaa tggctggatc
      121 cggcgcgctg gcgcggacat cggaatgatg atgacgccga cttcgcaagg ccgcattccg
      181 gccgccggcc tgccgtggct cgatgccatc ttcggccggg acgccatcat caccgcgctc
      241 gagacgctgt ggttgtggcc ggagatcgcg cgcaacgtgc tgaggtatct cgccgccaca
      301 cagtgcacca gcagttccag tggttctggc gccgagatcg gcaagatact caatgagtcg
      361 cgcgccggct ttccgactga ctactacggc gtggacacca cgccgctgtt cgtgatgctg
      421 gccggcgcct actacacgcg cactgccgat ctcgacctga tgaccgagct gtggccagcg
      481 atcacggccg ctctgcgctg gatcgatgtc tatggcgatg tcgatggcga tggcttcgtc
      541 gaagcccggc cgcgcggtac cggccccgcg cagcacagct ggaagagctc tccggatgcc
      601 gtctttcacg ccgatgggcg tctggcgacg gggccgctgg ccatgtgcga agtgcagggc
      661 tacgtctatg ccgcgcgact cggcgcggcg cgcattgcgc gctcactcgg cgaggcgcca
      721 cgcgccatct cgctggtgga atccgcgcag gcgctgcaag cgcgattcaa taccgcgttc
      781 tggtgcgggg acatcgaggg atatgcgctg gcgctcgatg gtgaaaagca accctgccgc
      841 gtgcgcgcct ccaatccggg acattgcctg ttcacgggca tcgcacactc cgagcgtgcg
      901 cgacaggtca tcgcggggct cggcgacgag catttctttt cgggctgggg cgtgcggtcg
      961 gtgggcgaga gcgagttgcg ctacaacccg atgtcgtatc acaacggttc ggtctggccg
     1021 catgacaacg cgctgattgc ggcgggcgcc gcgcgttacg acgacaagac gttcgcgctg
     1081 cgcatcctga atgcacagtt cgaagctgcg cgacactttg agctgatgcg cctgccggaa
     1141 ctgttctgtg gtttccgacg caagggccag gaggcgcctc tgcatttccc ggtcgcctgt
     1201 tcaccgcatt ccgtgtctgc cggcgccacc ttcctgttgc tccagagcgt gctgggtatc
     1261 agcatcgatg cactcgatcg ccagatcgtg ctcgcgcatc cggtggtgcc cgacggcttc
     1321 gacgcaatct gcgtgaaaaa cctcgaggtg caggatgcgt cggtggattt caccgtgcgc
     1381 cgccacggtg gttcggtgag cgtgagcgtg gagagaaggg aagggaaggt ggatctggtg
     1441 atccggagct gatgcgcgag gggtgcttcc ccgcccgcca tgtcacacca tgctgtcagc
     1501 ggcttcctac gcgattgtgc ggagcagtcc cgcagggacg ccatttagac tctgacgcac
     1561 ctcgcaggaa cccaggcatt ccatggaaat ctccgcacgc ggacgcacta cggcggcgcg
     1621 ccgccgcacg ccgcaggtta acaagaccaa gcctgcgaac ggaaatggtg gtagcggaca
     1681 cactaccgcg ccagaactgg atctgaagaa gttactgcgt gcactgcagg cggttcgcga
     1741 cggcgatttc agcgtccgcc tcgctccgga tcagacggga ctcggcggca agatcgccga
     1801 tgcattcaat gaaatggtga gcgcgaatga gcgcatggcc accgagctcg agcgcgcggg
     1861 ccagatggtc ggtaaggaag gcaagacgcg agtgcgcgta tccagcgaac gccgcaccgg
     1921 ggcctggagc gagatggaat cgtccgtcaa cacgctcatc gacgatctgc tgtggccaac
     1981 ggccgaagtc acccgcacga tcacggcggt ggccaagggc gatctcacgc agagcatgcg
     2041 gctcgaagcc gacgggcgcc cattgaaggg agagttcctg aggtcggcaa atgtcgtcaa
     2101 cgagatgatc acgcagatgg gcgtgttcac ctcggaagtg aacacaccca tctgcgtgat
     2161 catctcgttg accacgttcg cggatcgcag gaattcgccc ttcagcggac gtccatcggc
     2221 ttcgagccgc atgctctgcg tgagatcgcc tttggcgacc gcggtaatgg ttcgcgtcac
     2281 ttcggcggtg ggccacagca gatcgtcgat gagcgtgttg acggacgatt ccatcgcgcc
     2341 ccaagaaccg ctgcgacgct cggccgacac gcgggtgcgc gtcttgcctt ccttgccgac
     2401 catctgcccc gcgcgttcga gctcggaggc catgcgctcg ttggcgctca ccatttcgtt
     2461 gaatgcatcc gcgatcttgc cgccgagccc ggtctgatcc ggtgcgaggc gcacgctgaa
     2521 atcgccgtcg cgcaccgctt gcagcgcgcg aagcaatttc ttcagatcca ggtcgggtgc
     2581 ggctgcgggg ccgcctcctc cattcccgtt ggcaaccttt gctttggcgt cagctggcgt
     2641 gcggcggcgc gcagttgcgg cgcgtccgcg tgcggagatt tccatggttt gtctgatcta
     2701 cccgggagcc tacgaggtgc gtcagagtct aaatgccgtc cacgcgggac tgctccgcac
     2761 actgatgtag gaggcgcctg acaaggtgga cgcggccgct cgcgaggcgc ggccccctca
     2821 gctccggatc acgaggtcca ctttgccttc ccgtctctcc acgctcacgc tgaccgaccc
     2881 ggtatgccgg cgcacggtga agtccaccga cgcatcctgc acctgcaggt tcttcacgca
     2941 gatcgcatcg aacccatcgg gcaccaccgg gtgcgccagc acgatctgcc ggtcgagcgc
     3001 atcgatgccg atgccgagca cgctctggag cagcaggaac gtggcgcccg ccgaagctgc
     3061 ctgcggcgta caggcgaccg gaaactgcag cggcgcctca cggccgcggc gcttgaagcc
     3121 gcagaacagc tccggcagcc gcaacaggtc gaaatgccgg gcggcctcgt attgcgagtt
     3181 caggatgcgc agcgcgaagg ccttgtcgtc ataacgcgcg gcgccggctg cgatcagcgc
     3241 gttgtcatgc ggccacaccg agccgttgtg atacgacatg gggttgtagc gcagctcgct
     3301 ctcggccacg gttcgtacgc cccaccctga aaagaagtgc tc
//
LOCUS       DASQJS010000137         5628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1013934, whole genome shotgun sequence.
ACCESSION   DASQJS010000137 DASQJS010000000
VERSION     DASQJS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013934"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /locus_tag="VEW08_05650"
     CDS             complement(<1..1718)
                     /locus_tag="VEW08_05650"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HYJ40254.1"
                     /translation="MSAWRYIYKSVRVVVLALFVWAGAAGAYADERILSFDETVTVNA
                     DGSLYVRERIRVRAEGRKIRRGIYRDFPTTYSGSDGHQYVVEFAFQGASRDGQAEPWR
                     AENRGNGVRIYIGNASKLVQPGEYTYELIFRTDRQLGYFADHDELYWNVTGNGWDFPI
                     DRVTARVELPQAIPATEIKLEAYTGPQGAKGQDYTAQMQASGPLFATTRGLRPNEGLT
                     IVAMWPKGFIMPPVESTRSAAAPPPASYMNDDGRSGWSPAEHLLDRDLPDNGMPALFA
                     ALGLSLLIIYYYFIWARVGRDPPGRITIPEYEFPAGQSPASMRFLMRMGYDNECFAAA
                     VLSLAVKGYLRIHEDSGVLGFGRTFTLHKTSDQDGKPLTADEQQLLTSLFSRGSTLVL
                     ESKNHERVSKARTGHNRAIKERFSSGFFKINGGWHLLGIVLSLVLAAPAVLLPGRTDH
                     YPEWHFTTPGGWFTIAAVLAMLVANGVFGKLLKAPTVAGQSIMDHIRGFKMYLEVAEG
                     EELKRMKAPPPPLTPQLYESYLPAALALGVEQRWAERFASVLDVQAPNYAPAWYAGSG
                     WNIHNMG"
     gene            complement(1715..2266)
                     /locus_tag="VEW08_05655"
     CDS             complement(1715..2266)
                     /locus_tag="VEW08_05655"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HYJ40255.1"
                     /translation="MLTTFIVLVVAALLWAAIVFNLLIRDRNRVAQAWSDVDVQLMRR
                     HDLIPRLVDMVKGYAGYERALLTSLAEMRGQSANADSARQRSEVEKGVSNGVTRLVAL
                     AEAYPDLKASENFRDLQNKLVDTENQIQFARRYYNGAVNLFNNRIQKFPDMLIANSFG
                     FKAAEFFDLEDPKAAAAPAVAMP"
     gene            complement(2300..3040)
                     /locus_tag="VEW08_05660"
     CDS             complement(2300..3040)
                     /locus_tag="VEW08_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYJ40256.1"
                     /translation="MNVGEKIRQLRTERNLTQPQLAESIGIEQSYLSKLENDKSVPSA
                     DIFQAILKAFGVDLATFLQGIDERIVHGDLRQIPEVSHHINAKLACRVHNIRRWLFGS
                     AVACVVGLSLVVAGAMGLIFSNEQYTYYSPGVVISGEATNIFSMHNHLLLAALAAGTI
                     THDERAKQMVAFNTHRVREDYLLSDSYRGEAFRVAVRDGSRGYELKKVAEHERVANRY
                     LTLFGSLLAFGGLFGFFVEYRLRKIIPG"
     gene            complement(3137..3595)
                     /locus_tag="VEW08_05665"
     CDS             complement(3137..3595)
                     /locus_tag="VEW08_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40257.1"
                     /translation="MSAVTIKESRRLLLPLETVLEALVHFDRRSNGPLSRGDVLQADF
                     VSDREGGDAIEVAVRMPGTSDIEWRRFDFGAIAAAIISFCHAKRIPLPLSGVKSIALT
                     QEGISFSIENQVNIAQRADVRSDIAGRPIQYAKGYEPHSIRPSGENVALA"
     gene            3738..4274
                     /locus_tag="VEW08_05670"
     CDS             3738..4274
                     /locus_tag="VEW08_05670"
                     /inference="COORDINATES: protein motif:HMM:NF018813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HYJ40258.1"
                     /translation="MALGATIYTFDIELADSDRAVYETLNLRVAQHPSETPDYLITRV
                     LAYCLEFSEGIAFSKGLSDPDEPAICVRDLTGALQVWVDIGLPEPERVHRASKAAPRV
                     AVYTHKDAAQWAMRIRDAKIHRAEKLEAFAFERAWLSAVVARLERRMKFSLARSEGEI
                     YLTLGADTSQTVLERPLD"
     gene            4342..5493
                     /locus_tag="VEW08_05675"
     CDS             4342..5493
                     /locus_tag="VEW08_05675"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYJ40259.1"
                     /translation="MLVSRRALLIGGVTLPVVSKAVNTPASAAARIDEARAVVTAMLA
                     AEPIPALSIAVIQGKEVIWAEAFGKANLDLGVPATTAHRFRLGSVSKILTATLAARLA
                     TRGVVDLDAPIANYLPDLPEQHRATTLRQLLTHRGGIRHYLDKDSMPGPAGAPIDYRD
                     YASNAEVLAIFINDPLVAKPGERVSYSTFGYTLISIVLETAAKTPFLELIQSEIARPM
                     ALASVGPDAPRAITANRVTGYHTARIAGAVHPPLEGPWANILPVNPAYKWAGGGLLAT
                     PTDLARFGAAHWAPGALDQRTLTDLFTIQTQATERSPPLGLGWRIDEDPNKRLRWHHA
                     GGQDGARASLVVYPKEKLAIAFATNLTGAPGDVNGPSAKIANALLEIRT"
BASE COUNT         1063 a         1814 c         1705 g         1046 t
ORIGIN      
        1 cccatgttgt gaatgttcca gccggaacct gcgtaccacg cgggagcgta gttcggcgcc
       61 tgcacgtcga gcacgctggc gaagcgttcg gcccagcgct gttccacacc gagcgccagc
      121 gccgccggta gataggattc gtagagctgc ggcgtgagcg gcggaggcgg cgccttcatg
      181 cgcttcagtt cttcaccctc ggccacttcc aggtacatct tgaagccgcg gatatgatcc
      241 atgattgact gcccggccac cgtcggcgcc ttcagcaact tgccgaacac gccattggcc
      301 acgagcatcg cgagcaccgc agcgatggtg aaccagcctc caggcgtcgt gaaatgccac
      361 tccggatagt ggtccgttct gcccggcagc agcacggcgg gcgccgccaa caccaggctg
      421 agcacgatgc cgagcagatg ccagccacca ttgattttga agaagcccga cgagaagcgt
      481 tctttgatgg cgcgattgtg gccggtgcgc gccttgctca cccgctcgtg gttcttgctc
      541 tcgagcacca gcgtgctgcc gcgtgagaac aggcttgtca gcaactgctg ctcatcggcc
      601 gtcagcggtt tgccgtcctg atcgctcgtc ttgtgcagcg tgaacgtgcg gccgaacccc
      661 agaaccccgg agtcttcgtg aatgcgcaga tagcccttca cggcaaggct cagcactgcc
      721 gcggcgaaac actcgttgtc ataccccatg cgcatcagga atcgcatgga ggccggcgac
      781 tggccggcgg gaaattcgta ttcagggatg gtgatgcgcc ccggcgggtc acggcccacc
      841 cgcgcccaga tgaagtagta atagatgatc agcagcgaca gacccagcgc ggcgaacagc
      901 gccggcattc cgttgtcagg caggtcccgg tcgagcaggt gttccgccgg tgaccagccg
      961 ctccggccgt catcattcat gtagctagca ggcggcggcg cagccgcgga gcgtgtggac
     1021 tcgaccggag gcatgatgaa acccttcggc cacatggcga cgatcgtcag gccttcattg
     1081 ggccgaaggc cgcgcgtagt cgcgaacagc ggcccactgg cctgcatctg ggccgtgtag
     1141 tcctgcccct tcgctccctg cggtccggtg tacgcctcga gtttgatctc ggtcgctgga
     1201 atcgcctgcg gcaattccac gcgcgccgtg acgcgatcga tggggaaatc ccagccgttg
     1261 cccgtgacgt tccagtacag ctcatcgtga tcggcgaagt agcccagctg gcggtcggtg
     1321 cggaagatga gctcgtaggt gtattcgccg ggctgcacca gcttggaagc gttgccaatg
     1381 tagatgcgca caccgttgcc gcgattttct gcgcgccatg gctccgcctg gccatcacgc
     1441 gatgcgccct ggaacgcaaa ctccaccacg tactgatgac catcgctgcc gctataggta
     1501 gttggaaagt cacgatagat gccgcgacgg atcttgcggc cttcggcacg cacgcggatg
     1561 cgttcgcgca cgtacaacga gccgtccgca ttcaccgtta cggtttcgtc gaacgacagg
     1621 atgcgttcgt cggcgtaggc gcctgcggca cccgcccaga cgaacagcgc caataccact
     1681 acacggacac tcttatatat gtatcgccag gcgctcatgg cattgccacc gccggagccg
     1741 ccgccgcctt cgggtcctcg aggtcgaaga attccgcggc cttgaagcca aagctgttgg
     1801 cgatgagcat gtccggaaac ttctgaatgc ggttgttgaa gagattcacc gcgccgttgt
     1861 aatagcggcg cgcgaactgg atctggtttt ccgtatcgac cagcttgttc tgcaggtccc
     1921 ggaaattttc gctggccttg aggtctgggt aggcttcggc caatgccacc aggcgggtaa
     1981 cgccgttcga aacgcccttc tccacctcgg agcgctgcct ggcggaatcc gcgttggcgc
     2041 tttgccctcg catctccgca aggctggtca gcaacgcgcg ctcgtagccg gcgtagccct
     2101 tcaccatgtc caccaggcgc gggatgagat cgtgacggcg catgagctgc acgtcgacgt
     2161 cgctccaggc ctgggccacc cggttgcgat cgcggatcag caggttgaag acgatggcgg
     2221 cccacagaag ggccgccacg accaggacaa taaaggttgt gagcatctgc gccatgctaa
     2281 tggccgcgga cgcacggcgc tatcctggaa tgattttccg caaccggtac tccacgaaga
     2341 agccaaacaa cccgccgaac gcgagcaggc ttccgaacag cgtgaggtag cggttcgcca
     2401 cgcgttcgtg ctcggcaacc ttcttcagct cgtagccgcg ggaaccgtcg cggacagcca
     2461 cccggaatgc ttccccgcga taactgtcgc tgagtagata gtcctcccgt acccgatggg
     2521 tattgaatgc gaccatctgc ttcgcgcgtt catcgtgggt aatcgtgccg gcggcgagcg
     2581 ccgcaagcaa aaggtggttg tgcatcgaaa aaatgttggt ggcttcaccg gatatgacta
     2641 cccccggcga gtagtacgtg tactgctcgt tcgaaaagat gagccccatg gcgccggcga
     2701 cgaccagcga caaccccacc acgcacgcaa ctgccgatcc gaacagccac ctgcggatgt
     2761 tgtgaacccg acaggccagt ttggcgttga tgtgatggct cacctcggga atctgccgca
     2821 ggtcgccgtg cacgattcgc tcatcgatac cctggaggaa ggtggcaagg tcgacgccga
     2881 acgccttgag gatggcctga aagatgtccg cggagggtac cgacttgtcg ttctcgagct
     2941 tggacaggta cgactgctcg ataccgatcg attccgccaa ctgaggctgg gtaaggtttc
     3001 gctcagtcct tagctgcctg atcttttcgc caacgttcac gttctgctcc ctgttagaag
     3061 gacccggagc cgtatgctcg ggaattcctg cggagattag aagatgaata ttggcgaata
     3121 gcgatgaatc tggcggtcag gcgagcgcga cgttctcgcc cgaaggcctg atcgaatgcg
     3181 gctcgtatcc cttggcatat tgaatcggcc ggcccgcgat atccgaacgc acgtcggcgc
     3241 gctgagcgat gttcacctgg ttctcgatcg aaaaggagat gccttcctgc gtgagcgcga
     3301 tcgacttcac gccggacaag ggcagcggaa tgcgcttcgc gtggcagaag ctgatgatgg
     3361 ccgcggcaat ggcgccgaaa tcgaagcggc gccactcgat gtcactcgtg cccggcatgc
     3421 gcaccgccac ttcgatggcg tcacccccct cccggtcaga cacgaaatcc gcctgtagaa
     3481 catcgccacg cgacaatggc ccgttcgagc ggcggtcgaa atgcaccagc gcttcgagga
     3541 cggtttccag cggcaacagc agtcgccggc tttcctttat ggtcactgcg gacatggggc
     3601 cagagtattc tcgctcggga aaactcctag taccgggcca caataatccg catgtaaagt
     3661 ggatatcacg cacgggatgt gaggcccgtc acgctcgggc ggcctcaact gccgctcatt
     3721 ttgttaccgg aggtctcttg gcgctcggcg caaccatcta cacattcgat atcgaactcg
     3781 ccgattctga tcgcgccgtc tacgaaacgc tcaacctgcg cgtcgcgcaa catccgagtg
     3841 aaacacccga ctacctgatc acgcgggtgc tcgcgtattg cctggagttc tccgagggca
     3901 tcgcgttttc gaaaggcctt tccgacccgg atgagcccgc aatctgtgtg cgcgacctca
     3961 ccggtgcgct ccaggtctgg gtcgacatcg gcctgcccga gcccgagcgg gtgcatcgcg
     4021 ccagcaaggc ggcgccgcgc gtcgcggtgt acacacataa ggatgccgcg cagtgggcca
     4081 tgcgcatccg cgacgcgaaa atccaccgcg cggagaagct cgaggcgttt gcgttcgagc
     4141 gtgcgtggct gtcggccgtg gttgcccgcc tcgaacgccg catgaaattt tcgctggccc
     4201 ggtccgaggg cgagatctat ctcacactgg gcgccgacac ctcgcagacc gtgctcgagc
     4261 ggccgctgga ttgaaacttt caaacgaaag atgccgcaag cgcatctaca gtctcacgcg
     4321 tttcctcccg gggagataaa catgctcgtc tcacgtcgcg cgctgctgat cggcggcgtt
     4381 acccttcctg tcgtcagcaa agccgtcaac acgcccgcgt cggcagcggc tcgcatcgat
     4441 gaagcgcgtg cggtcgtcac ggccatgctg gccgcggagc cgattcctgc gctgtccatc
     4501 gcggtgatcc agggcaagga agtcatctgg gccgaggcct tcggcaaggc caatctcgac
     4561 ctcggcgtgc cggcgacgac tgcacatcgg ttcaggctcg gcagcgtatc caagatactc
     4621 accgccacgc ttgccgcccg gcttgcgacg cgcggagtcg tcgacctcga cgcaccaatt
     4681 gcgaactacc tgccggacct ccccgagcag caccgcgcca ccacgttgcg ccagctcctc
     4741 acgcatcgcg gtggcattcg ccactatctg gacaaggatt cgatgccggg ccctgcggga
     4801 gctcccatcg actaccgcga ctacgccagc aatgccgaag tgctcgcgat cttcatcaac
     4861 gatccgctgg tcgccaaacc tggcgagcgc gtgtcgtatt ccacgttcgg atacacgctg
     4921 atttcgatcg tgctcgaaac cgcggcaaaa accccgttcc tcgagctcat acaaagcgag
     4981 attgcgcggc caatggcact ggcctcggtc ggccccgatg cgcctcgcgc cattacggcg
     5041 aatcgcgtta cgggttatca cactgccagg atcgccggtg cggtacatcc gccgctcgaa
     5101 ggcccgtggg cgaacattct tcccgtcaat cccgcttaca aatgggccgg cggcgggttg
     5161 ttagccacgc ccaccgatct tgcgcgtttc ggcgccgcgc attgggcgcc gggtgcgctc
     5221 gaccagcgca cgctcactga cctgttcacc attcagacgc aggcgaccga gaggtcgccg
     5281 ccgctcggcc tcggctggcg catcgacgag gatccgaaca agcggctgcg ctggcaccac
     5341 gccggcggcc aggacggcgc gcgcgccagc ctcgttgtgt atccgaaaga gaaactggcg
     5401 atcgcgttcg ccaccaacct caccggggct ccgggcgacg tgaatggacc gagcgcgaag
     5461 atcgccaatg ccttgttaga gatacgaaca taacgggcca cctgttattt cgctcacagt
     5521 ccggggactg ccctagggcg gaccgcacag aattccggca attcgattgc cggcagccgg
     5581 cgcgcgaaat agtttcgagc catgaaaact tcaagccttc gtcgactg
//
LOCUS       DASQJS010000138        18997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4154359, whole genome shotgun sequence.
ACCESSION   DASQJS010000138 DASQJS010000000
VERSION     DASQJS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18997
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4154359"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VEW08_05680"
     CDS             <1..853
                     /locus_tag="VEW08_05680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HYJ40260.1"
                     /translation="TGMLILDKDGAVQSCNPAFVRILGPAASVPQAPLRDLLAPHGAT
                     VAKLIEASQASGEPREADLEIDLAGPRGKVWVELSVNPLGPNLLQALVNDITERKRAE
                     SAAQELATRDTLTALLNRRGFDTALAGLMANSRRDSRLALLQIDLDYFKSVNDNYGHE
                     AGDLVLREVARIFERSVRRSDIVARVGGDEFAVLLPGIESAAKAEQIARNIISGVKVP
                     IDIGGGRLAQVGASIGIALLGDAPESPAAFARRADLAMYAAKQAGRGCAYIAPAANDE
                     PEATAAA"
     gene            964..2067
                     /locus_tag="VEW08_05685"
     CDS             964..2067
                     /locus_tag="VEW08_05685"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HYJ40261.1"
                     /translation="MKLFSCDNCQQVLFFENSRCMNCGMNVGYCVELAKIVTLQHQTG
                     SSQGSVYEVFFPNQGLRHYRQCKNGRDHDACNWLVPAESQQQFCNSCRLTEIAPDLSN
                     TNNLTAWAGIEAAKRRLLYTLYQLKLPLSSRQEWPNEGVAFHFLRSANGEPVMTGHDE
                     GVITLNIAEADSAFRENMREKMGEAYRTVLGHLRHEIGHYYWDRLIRNSPSLQGFRNL
                     FGDDSRDYDEAVQRHYEQGAPANWAESYISAYATMHPWEDWAETWAHYLHMIDTLETA
                     KSHALSLRVPAAERTGDQISTDTVAFRDFDSLTSNWNAVTLALNSLNRSMGLKDAYPF
                     VLSPRVLEKLRFVHDLIQSRRAEQKSESIRATA"
     gene            2100..4553
                     /locus_tag="VEW08_05690"
     CDS             2100..4553
                     /locus_tag="VEW08_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HYJ40262.1"
                     /translation="MRRHMFLALGFSLGPVAVVAADDFKLNAQEYFERPGINVMYGQD
                     YYPEGHQGGLSIIMHDERIASNGDVRLEPSPGQWAPIPKPGKREINATTQEIRVALAY
                     PDAERDRKGFNPIVYPDLKLNYTVRARPEGESIRVSVDFDAPIPKEWVGKVGFNFELF
                     PGLLFGKSYQLGKTEAVFQRQPSGPGEIPILARGAKLEVAPETELYHLTIEAVRGGEL
                     TLLDGRGNHNNGWFVVRALAPAGATTGAIEWLVTPHAKAGWLRTPVIQVSQVGYHPAQ
                     PKQAILEFDIRDTKRENVILQRIGQAGPVKVVVDRRPAEWGRFLRYQYAILDFSDVKE
                     PGLYVVRYGGQTSHPFRINADVFTREVWQPTLEYFLPAQMCHMKVLEKYRLWHGACHL
                     DDARMSPVNHNHFDGYLQGPTTLTNYQPGEHVPGVDRGGWHDAGDWDMRVESQADTIY
                     GLALAWELFHVDYDNTSIDQKTLVTEIHQPDGRPDVLQQLEHGVLSVVGGHESLGRLY
                     RGIIEPTLRQYVFLGDGALMSDNVVFDQGKTREEGAPEVGLPGSPDDRWVFTEENPRR
                     ELLTAASLAAASRALKGYNDDLAARALKAAIAIYDRATPKEPIARVGAATELYITTRD
                     PKYRALLLEQRDAIAADIERVGWVVGRTLPLIKDKAYSKTIRDAVAAYRLKVDALAKE
                     TPYAVPYKPDIWGAGWNIQRFGWDQWMLHLSFPDLFPRTYALQALSFILGVHPGSNTA
                     SFVSGVGAHSLTTAYGFNRADWSYIPGGSASGTALIRPDFPELKTWPFFWQQTEYVLG
                     RGTTDYLLLVLAADHVLNQ"
     gene            complement(4562..5812)
                     /locus_tag="VEW08_05695"
     CDS             complement(4562..5812)
                     /locus_tag="VEW08_05695"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HYJ40263.1"
                     /translation="MSSEATPVSPRERIYTLDVIRGFALLGIFIMNMPGFNTSFYANF
                     AGSDLFPANYDRWAENLRDVFFSGKFNSMFSMLFAIGFTIQLERLEARDPAHAKSIYL
                     RRIFWLFVFGAIHMCVFWTGDVLHIYALFGLLLLALRRAPERLLWTLFAFCMLFPVAM
                     GLYRLMVFTPEFKDYVVAVSKAWEATNNAAYGQGSWLDAAREHYRETVILYSEPFMMR
                     GMLGFYVQVFSTMVLGLMLGRRQFFQNAALHLPFVKRMQWSMLAFGIATGAVFGIWQA
                     TTTDFVTPTPARIVAIVCYFVCRVAIMIFYVATIVRCVHNDAWRRRLAPMASAGRMPL
                     TNYLMQTLMATFIFYGWGLGFWGKAGPALDLLLAFVLFFAVQVPLSKWWLSRYELGPM
                     EWLWRRLTYGKVKLSLRQGSAAPT"
     gene            5877..7046
                     /locus_tag="VEW08_05700"
     CDS             5877..7046
                     /locus_tag="VEW08_05700"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYJ40264.1"
                     /translation="MNIRSLTFLWFCLIAPLAGVAAEPAAFDSAIRDLGRAWLARNDG
                     VGLSIGVYENGQRHFYNLGATALDGNKTPTKETIYEIGPLAKTISGQLLARAIVEGRA
                     SINDEVTKYLEEPYPNLENGGEPVRLLHLANMTSQLVDNIPDVSQVRTVPGEPLTTTR
                     MRVLERYTRKEFLRQLHMVKPRRPPGSDPYHSNVASMLLGVVLEKLFDESFDTILSRE
                     IEKPLRMGSGTAPLTKLLARGYTNEGDAVPPFTAPTQYSSASLRYSTEDLLNYAAWQM
                     VERDASVKLAHQPTWTTPDKRYAVGFYWIVTQSPAGRRLQYSGSTYGFASACDLYPDA
                     KVAVVLLSNKAADGAQESLRAMSAKIVELVAPGKPTAPTGAVSPPSSAGALPPGH"
     gene            complement(7003..7470)
                     /locus_tag="VEW08_05705"
     CDS             complement(7003..7470)
                     /locus_tag="VEW08_05705"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HYJ40265.1"
                     /translation="MSKNFAEPGKMAPPAAPQKEVAAGTCILGPTLHFKGELYGEEDL
                     EFQGQLEGSIEHTRSLSIGKEGGVTGNIKAKFVIVEGRVDGDIYASESVSIRQSAKVK
                     GNIFAPRVGIADGANFSGSIDMNHKGAAVATRPATVQVTLSDPAAERLLTKAG"
     gene            complement(7484..7855)
                     /locus_tag="VEW08_05710"
     CDS             complement(7484..7855)
                     /locus_tag="VEW08_05710"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HYJ40266.1"
                     /translation="MNASNERMSVFGPTLRFKGELKAQEDIKIEGRIEGTIHHQQRVI
                     VGAKGEVVAAVNAASIDVDGKVQGDMVARKSVKVSQTAVVRGNIRAPSVSITEGANFN
                     GGVTMESNAGSQGSSTPGASR"
     gene            complement(8032..8667)
                     /locus_tag="VEW08_05715"
     CDS             complement(8032..8667)
                     /locus_tag="VEW08_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ40267.1"
                     /translation="MKRPRGRPPHADLLTPSEWRTVHAVQHGMTNAEIARRRGVSVNA
                     VKYHLANALAKLNLRNRRELRAWFRAPKGRFTMAVEAKLGPIGQIARSVSDIQASETW
                     YREVLGLPHLYTFGTLAFFDCGGTRLMLSQEGGAAKESILYLRVGDIAAAHQQLAARG
                     VKFTHAPHMIHRHANGIEEWMAFFEDPDGRPLALISQVPGGESGEKKPRTP"
     gene            8783..10537
                     /locus_tag="VEW08_05720"
     CDS             8783..10537
                     /locus_tag="VEW08_05720"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HYJ40268.1"
                     /translation="MTERENADESAEDSAGAGVAVALSALTLAACGGGGGSSTPPPVQ
                     NPPPVVPPVVPPPVTITDAEAARFLLQAQFAVTDTDLAAVKANGYAAWLTTNYNAAPG
                     QTGVAWLDSRGHNSITSEQRYFWPQFGDFMIWNQLLAGPDQMRKRFAFALSEFFVVSL
                     SPIDGFYPPYVIAAYWDVLCANAYGNFRTLLEAITLNAGMGFFLNTKGNLKEDANGRQ
                     PDENYAREVMQLFTIGLYELNADGTQRLDANNQPIETYGQSDITNLARVFTGYDWDYT
                     GNVNTTVAWHPYPVPNTRFATNPMRFIPGNHSNLAATFLGTTIPANTPGPEALRIALD
                     RLFNHANTGPFFARQMIQRLVTSNPSPAYVGRVAAAFADNGSGVRGDMKAVWTAILND
                     TEARTLPAASDTLSGKLREPVVRLVQWARTVGITSSNGAYEIYDLSGSDTSLGQSPLR
                     SPSVFNFFRPGYVPPNTAIAAAGKQAPEFQLLNETTTAGYINFMQGMTRNGYYSFDVN
                     PSYTTLMTIAHDVPAVIAWLNLRLTANQLSATTLAVLQATLSAFNVTAGSAENLKLDM
                     LATACFLILCSPEYLVQK"
     gene            10534..11946
                     /locus_tag="VEW08_05725"
     CDS             10534..11946
                     /locus_tag="VEW08_05725"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HYJ40269.1"
                     /translation="MKRPDVNAPGLWTRRVLLQRALHLGAMGVATPLAINLAAIGEAA
                     AFDNTDYKALVCIFLYGGNDYANTVVPYDATNYALYHQIRAGAVGENQAGIALARAAL
                     EPTALTPNNGQVLTDNLQYALAPQLTGLKSIWDAGRLAVQLNVGPLVQPTTLAQYQST
                     NRVANPLPPKLFSHNDQQSVWQSNGTEGSTVGWGGKLGDIAMSSNATNSLLTCISASG
                     NAVFVAGRDALQYQVSPGGAVRINALNTPLRDALNAIITRQSSHVLENEYAIVTRRSM
                     DMEGVVNTALSGVNPTTPFGTGNGLANQLKIVARLIGARATLGMRRQVFFVSLGGFDN
                     HNLLMQDHPNLMTRVNEAMSAFYAATVELGVAEKVTTFTASDFGRTLSSNADGSDHGW
                     GSHHFVMGGAVNGGRFYGTAPHVSIQTDDQVGQGRLLPSTSVDEFAATLARWFGCNAG
                     ELPGILPNVGNFTNTDLGFV"
     gene            11981..14728
                     /locus_tag="VEW08_05730"
     CDS             11981..14728
                     /locus_tag="VEW08_05730"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HYJ40270.1"
                     /translation="MNDHDLSRRDFLAAAGGTAAMALAPASTIAAAGSAKPLNLAIVA
                     APSGSYVSADTRLTSLNSGATPGSSADPAQLAFGTAPEKGTQWAQYDWNEPISTNAID
                     VYWWGKHWGIALPVASRLLYWDGEQFVPVKNARGFGLEPNAFNRTEFDAVTTQRLRLE
                     IDPAADKSVGVLQWRVWSVGDVPKFAPVVTAGVDRTVMLGAPTYLLGRAAWLDRKRGD
                     AARWRKISGPGVVTFADADAAQTAAQVSAPGDYTFELSAQGDGFTTRSTLRLRAEMPP
                     PRDRLDVVYTTPYAILSPLWKARAKALIVNWIPHCVRYCERTDLKIGEGGLDNFIEAA
                     KALRGEPHGRHKGYVFSNAWIHQTIESICIALMVDAQGDQEILAAQAGMRTTLEKWIP
                     IVLAAQEPDGYLQTAYTLADRAKWPSRWNPGNRGDHEGYVAGYFIESAINHYTLTGGK
                     DLRLYIAAKKLADCWVANLGPGKKVWFDGHQEMEQALVRFGRFVNDMEGPLPGTNKGK
                     GDAYIALARFLLESRRGGSEYDQSHLPPQQQYEAVGHAVRAVYFYSGMADIAAETHDR
                     DYQSAVRSLADNIVNRKYYVTGGVGSGDTAEGFGGDYVLGNDAYCESCSSCGLVFFDY
                     KLNLAYHDARYADLYEETIYNALLGATDIEGRNFCYTNPLVNTERAAWHVCPCCVGNI
                     PRTLLMLPTWSYVKDRASVYVNLFVGSRINVGEVAGTPVEMVQETDYPWKGAVAITVN
                     PRDARTFSVKVRVPDRATSALYSATPSVKGLGKLRVNGAPLEVRIEQGYAVITREWRR
                     GDRIDFELPLTPQRITADERVAATRGKVALRYGPLLYNVELADQPRLDRALGQAPLTA
                     EWRGDLLGGVITLKGKWDDGSALTAVPNFVRMNRGEIPAREYPTEKPLDKVYSQVWMK
                     A"
     gene            14842..15570
                     /locus_tag="VEW08_05735"
     CDS             14842..15570
                     /locus_tag="VEW08_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40271.1"
                     /translation="MTLILVPALLALGGCATADVAMKLAPDLAATTALPVTGRDPGTQ
                     LAFGRWHVAMPAPYRMDGWQISGTPRFVPANSELSVKAERASLEFEVVADAGSRPASA
                     GCLAQGRFASLTTFHARSEDETSVTIPGFPRLDCEFSGARTGTLALRADFATQRDWGD
                     ARFGADHWLVRSVNTHAKQRSNFPLTRFGYEIVLGERVVAAVETYGAGRVWVLPDLSR
                     EQQEQLSTVAAALLYYGRLLDLQE"
     gene            complement(15617..16549)
                     /locus_tag="VEW08_05740"
     CDS             complement(15617..16549)
                     /locus_tag="VEW08_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40272.1"
                     /translation="MLKFFALAAGLMLASAASAQQSLEAMHARVPAPPATAAAAPAWL
                     ASPELVALRKQLKDQRTFIEKLSTEAGAAAQPTAAQTGGVIDFQRAQRDPAYAEQVKA
                     QIAAMSQEEKMKLAMQMQQATQQNALKDVKAMAADPEAVTAAADHYADYQANPSTMRG
                     IQAQYAAVADIQQRVRAREAEIASKAGKSLKCSDGEGGCASAADQAADKVTLKAAFDQ
                     IIAEYNKALPAIAQQVEAARKSRLAAITAAQADLGPAQYGSAAKSSANRQLLAAYHNA
                     VLIEVEQLMTLTEDAAKWAATRYKDRTINFTKID"
     gene            16725..17888
                     /locus_tag="VEW08_05745"
     CDS             16725..17888
                     /locus_tag="VEW08_05745"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HYJ40273.1"
                     /translation="MPLTRWTSIRFVYGLALAMLFASPVPASEQPPTLAIGAAMPPFS
                     LPGIDGKTYTEKSFASADILVMVFTCAHCPTAQAYQERIKKLVTDYASKNVTLIAINP
                     NHADAVRLDELAYSDLSDSFEEMVERAKQEKFNFVWLDDGPKQEMSHAYGPVATPHVF
                     IFDKARKLRFEGRIDDSERESSATKHDTRAALDALLKGKAPPVVTTKVFGCSVKWADK
                     VPDYANYRKKWAAEPVTLEKADAAMLKAIRENKDSGKIRFINVWATWCGPCITEFDEL
                     VEHNLRFRIRGFEMVTIAAEFPDQEPQVRDFLTKKHASMKNYIYATKDNDAMSEALDP
                     AWKGPLPYTIIVDEKGNVIYREMGSINFLAMRRAIVPALNRLTPWQGLSPIKP"
     gene            18010..18552
                     /locus_tag="VEW08_05750"
     CDS             18010..18552
                     /locus_tag="VEW08_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ40274.1"
                     /translation="MRTPILASAAAVSGAAGEDIAALARQYGPVVFRAAWRVLGGRAA
                     AEDVQQQVFLRLIESPPGKVESWPAYLTTVATRLSIDHLRRQRRWKLLAPLWKSDEPE
                     PDDSPAEDAERAEASRFMRAALARLNPRDASCFVMRHLHGLAPAAIGAALGLSENHVS
                     VSVHRARKALEAIAAKELSR"
     gene            18549..>18997
                     /locus_tag="VEW08_05755"
     CDS             18549..>18997
                     /locus_tag="VEW08_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40275.1"
                     /translation="MNSLDRTLELFAMREPDLALVNGAQHKLEALVAARVSTAAARRP
                     VRAARGWLAAAATAAVVAVAMLWLPFGSAPVLAFAKIQEHFRDFRTLRFDVEQRMDGR
                     LVMESRVSVTYDGNVRTDVGDDVTVIVNSTERRVLTLMHRSHVAIPSP"
BASE COUNT         3508 a         6119 c         6015 g         3355 t
ORIGIN      
        1 tacgggcatg ctgattctcg acaaggacgg cgcggtgcag tcgtgcaatc cggccttcgt
       61 gcgaatcctg ggcccggcgg catccgtgcc ccaggcgcca ctgcgggacc tgctcgcgcc
      121 gcacggcgcc acggtcgcga agctcatcga agcgagccag gcgtccggag agccgcgcga
      181 ggccgacctc gaaattgatc tcgccggacc gcgcggcaag gtctgggtgg agttgtcggt
      241 gaacccgcta ggtcccaacc tgctgcaggc gctggtcaat gacatcaccg agcgcaagcg
      301 cgcggagtcc gcagcgcagg aacttgccac gcgcgacact ttgaccgctc tgctgaaccg
      361 ccgcggtttc gatacggcgt tagccggctt gatggcgaat tcgcgacgcg attcgcgcct
      421 tgcactgctg cagatcgatc tcgattactt caagtcggtc aacgacaact atggacacga
      481 ggcaggggat ctcgtgctgc gcgaagtcgc ccggatattc gaacgcagcg tacggcgcag
      541 cgacatcgtc gctcgcgtgg gtggtgatga gttcgcggta ttgctgccgg gcatcgagag
      601 cgccgccaag gccgagcaga ttgcccgcaa catcatcagc ggagtcaaag tgccgatcga
      661 catcggcggc ggcagactcg cgcaggtcgg tgcgagcatc ggaatcgcgc ttctcggcga
      721 tgcgccggaa tcgcccgcgg ccttcgcgcg ccgcgccgac ctcgcgatgt acgccgccaa
      781 acaggcgggg cgcgggtgcg cgtatattgc ccccgcggca aacgacgagc ccgaggcaac
      841 cgccgcagcc tgaccgtgta ggagttttga cgcatcacag gcgatttccg ctgagttgct
      901 cgaaagtcca ttgcgaccaa catcctccgc gtccaagcca cgcgatgcga acccggagcc
      961 tcgatgaagc tattttcctg cgacaactgc cagcaagtgc tgttcttcga aaacagccgg
     1021 tgcatgaact gcggcatgaa cgtgggttac tgcgtcgagc tcgcgaagat cgtcacgctg
     1081 cagcaccaga cgggttcatc gcagggctcg gtctacgagg tgttcttccc caaccagggc
     1141 ctgcgccact accggcagtg caagaacggc cgcgaccacg acgcctgcaa ctggctcgtg
     1201 cccgcggaga gccagcagca gttctgcaat tcctgccggc tcacggaaat tgcgcccgac
     1261 ctctccaata ctaacaacct tacggcctgg gccggcatcg aagcggcgaa gcgacgcctg
     1321 ctctacacgc tgtaccagct caagctgccg ctgtcgtcgc gccaggaatg gccgaacgaa
     1381 ggcgtggctt ttcatttcct gcgcagtgcg aatggcgagc ccgtcatgac cgggcacgat
     1441 gaaggcgtca tcacgctgaa catcgccgag gcggactcgg cctttcgcga aaacatgcgc
     1501 gaaaagatgg gcgaggccta tcgcacggtg ctcggccacc tgcgccacga aatcgggcac
     1561 tactactggg atcgcctgat caggaacagc ccgtcgcttc agggattccg caacctgttc
     1621 ggcgacgact cccgggacta cgacgaagcc gtgcagcgcc actacgagca gggcgcgccc
     1681 gccaactggg cagagtcata catcagcgcc tacgccacca tgcacccctg ggaagactgg
     1741 gccgaaacct gggcgcacta cctgcacatg atcgacaccc tcgagaccgc caagagccac
     1801 gcactgtcgc tgcgcgtgcc cgcggcggaa cgaaccggcg atcagatctc caccgacacg
     1861 gttgcgtttc gcgacttcga ctcgctgacg tcgaactgga acgcggtcac gcttgcgctc
     1921 aacagcctga accgcagcat gggcctcaag gatgcatatc cattcgtgct gtcgccgcgc
     1981 gtgctggaga agctgcggtt cgtacacgat ctcatccagt cgcggcgcgc tgaacaaaag
     2041 tcggagagta ttcgcgcgac cgcttgagtt acccttcgcc tcacaacgaa ggggatcgca
     2101 tgcgtcgaca tatgttcctg gcccttggct tttctctggg gccggtcgcg gtagttgctg
     2161 ccgacgattt caaactcaac gcgcaggaat acttcgagcg gcccggcatc aatgtgatgt
     2221 acgggcagga ctactacccc gaaggccacc agggcggcct cagcatcatc atgcacgacg
     2281 agcgcatcgc ttcgaatggc gacgtccggc tcgagccttc tccggggcag tgggcgccga
     2341 tcccgaaacc cggcaagcgc gaaatcaacg cgaccacgca ggagattcgc gtcgcgcttg
     2401 cctatcccga cgcggaacgc gatcgcaagg gcttcaaccc catcgtttat ccggatctga
     2461 agctcaacta cacggtgcgc gcgcggcctg aaggtgagtc catccgcgtc agcgtcgact
     2521 tcgacgcgcc cataccgaaa gagtgggtgg gcaaggtggg tttcaatttc gagctcttcc
     2581 cgggtttgct gttcggcaag tcctatcagc tcggcaagac cgaggcggtc tttcagcgtc
     2641 agcccagcgg ccccggtgaa attccgatcc tggcgcgtgg agcaaagctc gaagtcgcac
     2701 ctgaaaccga gctgtatcac ctcaccatcg aagccgtgcg cggcggtgag ctcacgttgc
     2761 tcgatggccg gggcaaccac aacaacggct ggttcgtggt tcgcgcgctg gcgcccgcgg
     2821 gcgcaaccac cggggcgatc gagtggttag tcacgccgca tgcgaaggct ggctggctgc
     2881 gcacgccggt catccaggtg tcgcaggtgg gttatcaccc ggcgcagccc aagcaggcca
     2941 tactcgaatt cgatattcgg gatacgaagc gcgagaacgt gatcctgcag cgcatcggcc
     3001 aggcagggcc ggtgaaggtg gtcgtcgatc gcaggcccgc ggagtggggc cgattccttc
     3061 gctatcaata cgcgattctc gacttcagcg acgtgaagga gccgggcctc tacgtcgtgc
     3121 gctacggcgg ccagacgagc cacccgttcc gcatcaatgc cgacgtcttc acgcgcgaag
     3181 tctggcagcc gacgctcgag tacttcctgc cggcgcagat gtgccacatg aaagtgctgg
     3241 agaagtaccg cctgtggcat ggcgcctgcc acctcgacga cgcgcgcatg tcgccggtca
     3301 atcacaacca tttcgacggt tacctgcagg gccccaccac gctcactaac taccagcctg
     3361 gcgaacacgt gccgggtgtc gaccgcggcg gctggcacga cgccggcgac tgggacatgc
     3421 gcgtggaatc ccaggccgac accatctatg gcctggcgct cgcctgggag ctgttccacg
     3481 tcgactacga caacaccagc atcgatcaga aaaccctggt gacggaaatc caccagcccg
     3541 acggcaggcc cgacgtgctg caacagctcg agcacggcgt gctctcggtg gttggaggcc
     3601 atgaatccct gggccggctg taccgcggca tcatcgagcc aacgctgcgc cagtacgtgt
     3661 tcctgggcga cggcgcgctc atgtccgaca acgtcgtgtt cgatcagggc aagacgcgcg
     3721 aggaaggcgc tcctgaagtc ggcctgccgg gatcgcccga cgatcgctgg gtattcaccg
     3781 aggaaaatcc gcggcgcgag ctgctcacgg ctgcgtcgct cgccgccgcg tcacgcgcgc
     3841 tcaagggata caacgacgac ctggccgcgc gggcgctgaa agcggccatc gcgatttacg
     3901 accgcgcaac gccgaaggag ccaatcgcgc gcgtgggcgc cgcgaccgag ctctacatca
     3961 ccaccaggga tccgaagtac cgcgccttgt tgcttgagca acgtgatgcc atcgcggcag
     4021 acatcgaacg cgtgggctgg gtggtgggcc gtacgctgcc gctcatcaag gacaaggcct
     4081 attcgaagac tatccgcgac gcagtggcgg cctatcgatt gaaggtagac gcgctcgcga
     4141 aggaaactcc ctacgcagta ccttacaagc cggacatctg gggcgcgggc tggaacatcc
     4201 agcgcttcgg ctgggaccag tggatgctgc acctgtcatt ccccgacctg ttcccgcgta
     4261 cctatgcgct gcaggcactg agcttcattc tcggcgtgca tccggggtcc aacaccgcgt
     4321 cgttcgtttc gggcgtgggc gcgcattcgc tgaccaccgc ttacggattc aaccgcgctg
     4381 actggtcgta catccccggc ggcagtgcct cgggaacggc gctcatccgc ccggatttcc
     4441 ccgagctcaa gacctggccg ttcttctggc agcagaccga gtacgtgctc ggccgtggca
     4501 ccacagacta tctattgctg gtcctggcgg cggatcacgt gttgaatcag tagcgggcac
     4561 tctacgtggg cgctgcggat ccctgacgaa gactcagctt cactttcccg taagtcagcc
     4621 ggcgccacag ccactccatg ggcccgagct cgtagcgcga cagccaccac ttgctgagcg
     4681 gcacctgcac ggcaaagaaa aggacgaagg ccagcagcag gtcgagcgcg ggaccggcct
     4741 tgccccagaa ccccagcccc cagccgtaaa agatgaacgt ggccatcagc gtctgcatca
     4801 gatagttggt gagcggcatg cgcccggcgg aggccatggg cgcgagccgg cggcgccacg
     4861 cgtcgttgtg cacgcagcgc acgatggtcg ccacgtagaa aatcatgatg gcgacgcggc
     4921 agacgaaata gcagacgatg gcgacgatgc gcgccggcgt cggcgtaacg aagtccgtgg
     4981 tggtggcctg ccatattcca aaaaccgcgc cagtggcgat accaaacgcc agcatcgacc
     5041 actgcatgcg tttgacgaag ggcaggtgca gggcggcgtt ctggaagaac tggcggcgcc
     5101 cgagcatgag gccgagcacc atggtgctga acacctgcac gtagaagccc agcatcccgc
     5161 gcatcatgaa cggctccgaa tagaggatca cggtctcgcg atagtgttcg cgcgcggcgt
     5221 cgagccacga gccttgaccg taggcggcat tgttggtggc ttcccaggct ttcgagactg
     5281 ccacgacgta atccttgaat tcgggcgtga acaccatcag gcggtacaga cccatggcga
     5341 ccgggaacag catgcagaac gcaaacaacg tccagagcag cctttccggc gcgcggcgca
     5401 gggcgagcag cagcagcccg aacaaggcgt aaatgtgcag cacatccccg gtccagaaca
     5461 cgcacatgtg gatggcgcca aacacgaata gccagaagat gcggcgcagg tagattgact
     5521 tcgcgtgcgc gggatcgcgt gcttccagcc gctcgagctg gatggtgaaa ccgatggcaa
     5581 acaacatgct gaacatgctg ttgaatttgc cggagaagaa cacatcgcga aggttctcgg
     5641 cccaccggtc gtagttggcg gggaacaggt cgctgcccgc gaagttggca tagaacgagg
     5701 tattgaaccc cggcatgttc atgatgaaga tgccgagcag ggcgaatcca cggatcacat
     5761 ccagcgtata gatgcgttcc ctcggcgaga ctggcgtggc ttctgagctc atgacctgct
     5821 cccaagtaat cgggcgatgt tgttttggcg gagtgtacgc gcgaggatgc gcgccaatga
     5881 atatccgcag cctgactttt ctatggtttt gcctgatcgc tccattggcc ggcgtcgccg
     5941 cggagcccgc agcctttgac agtgccatcc gcgacctggg acgcgcctgg ctcgcgcgga
     6001 atgacggcgt gggcctgagt attggcgtct acgagaatgg tcagcggcat ttctataatc
     6061 tcggcgccac ggcgctcgac ggcaacaaga cgccgaccaa ggaaacgatc tacgaaatcg
     6121 gacccctggc caaaaccatc tccggacaat tgctggcgcg cgccatcgtc gaagggcggg
     6181 cgtcgatcaa cgacgaagtc acgaagtatc tcgaggagcc ctacccgaat ctcgagaacg
     6241 gcggggagcc cgtgcggctg ctgcacctgg ccaacatgac ctcgcaactc gtcgacaaca
     6301 tccccgacgt gagccaggtg cgcaccgtgc ccggtgaacc gctgaccacc acgcgcatgc
     6361 gagtgctcga gaggtatacg cgcaaggaat tcctgcgcca gctgcacatg gtgaagccgc
     6421 gccgcccgcc cggatccgac ccgtaccact cgaacgtggc gagcatgctg ctcggcgtgg
     6481 tgctggaaaa gctgtttgac gaatcgttcg atacgatcct gtcgcgcgag atcgaaaagc
     6541 cgctgcgcat gggcagcggc acggcgcccc tcaccaaact actggcgcgc ggatatacga
     6601 acgagggtga cgcggttccg ccgttcacgg cgccgacgca gtactcgtcc gcgtcgctgc
     6661 gctacagcac cgaagacctg ttgaactatg ccgcctggca gatggtggaa cgtgatgcgt
     6721 cggtgaaact cgcgcatcaa cccacgtgga cgacgcccga caagcgctac gcagtgggtt
     6781 tctactggat cgtcactcag tcgcccgcag gccgccgact gcagtattcg ggcagcactt
     6841 acggattcgc gagtgcctgc gacctgtatc cggacgcgaa ggtggcggtg gtcttgctgt
     6901 cgaacaaggc cgcggacggc gcgcaggagt ccctgcgcgc gatgtccgcg aagattgtcg
     6961 agctggtggc gccggggaag cccacggcgc cgaccggtgc tgtcagcccg ccttcgtcag
     7021 caggcgctct gccgccgggt cactgagcgt gacctgcacg gtcgccgggc gagtcgccac
     7081 ggccgcaccc ttgtgattca tgtcgatgct gccggagaag ttggcgccat ccgcgatgcc
     7141 gacgcgcggc gcgaagatgt tgcccttcac cttggctgac tgacggatcg agaccgattc
     7201 gctggcgtag atatcgccat cgacacggcc ttccacgatc acgaacttgg ctttgatgtt
     7261 tccggtcacg ccgccttcct tcccgatgga aaggctgcgg gtgtgttcga tcgagccttc
     7321 cagctggccc tggaactcca ggtcttcctc gccgtagagc tcgcctttga aatgaagtgt
     7381 cgggccgaga atgcaggtgc ccgcggcgac ttctttctgc ggagccgccg gcggcgccat
     7441 cttgccgggt tcagcgaaat tcttgctcat ggttttctcc ggctcagcgg ctcgcgccgg
     7501 gggtggagga tccttgcgac ccagcattcg attccatggt gactccgcca ttgaaattcg
     7561 ctccctctgt gatgctgacg ctcggggcgc ggatattgcc gcggaccacc gccgtctgac
     7621 tgaccttgac gctcttgcgg gcaaccatgt cgccctgcac tttgccatcg acatcgatgc
     7681 tcgcggcatt gaccgcggca acgacttcgc ccttggcgcc gacgatcaca cgttgctgat
     7741 gatggatcgt gccttcgatg cggccttcga tcttgatgtc ttcctgggct ttgagctcgc
     7801 ccttgaagcg cagcgtcggg ccgaataccg acatgcgttc attcgatgcg ttcatgaaat
     7861 ctccctgcgt tgaggcgcga agcgcggcat agattgcgcc ggctgtccta cgtaaaaccg
     7921 tgtcgcgatt cgcaggcgta cgcgattccg tcaaaggaaa aaacaatatg taacgtgggt
     7981 gccgggtggg gaaagtgggg agaacaagcc cttcatctga tttgctacaa ctcagggcgt
     8041 ccgcggcttc ttctccccac tttccccacc cggcacctga ctaatcagcg cgagggggcg
     8101 gccatcagga tcctcgaaga aggccatcca ctcttcgatg ccgttggcgt ggcggtggat
     8161 catgtgcggc gcgtgcgtga acttcacgcc gcgcgctgcc agttgctggt gcgccgccgc
     8221 gatgtcgcca acgcgcagat aaagaatgga ttccttggcg gcgccgccct cctggctcaa
     8281 catcaggcgc gtgccgccgc agtcgaagaa tgccagcgtg ccgaaggtat acaggtgagg
     8341 caggcccaac acctcgcgat accaggtttc cgaagcctgg atgtcgctga ccgaacgcgc
     8401 gatttgcccg atcggcccga gtttcgcttc aaccgccatc gtgaatctcc ctttgggtgc
     8461 gcgaaaccag gcccgcagct cgcgccgatt gcgcaggttc aacttcgcca gcgcgttggc
     8521 caggtggtat ttcaccgcgt tcacactcac gccgcgccgg cgcgcaatct cggcgttggt
     8581 catgccgtgc tgaaccgcgt gcaccgtgcg ccactcagac ggtgtcagca ggtcggcatg
     8641 cggcgggcgg ccgcgagggc gtttcatgag accaatcgta gtgtcacgcc cctcgatttt
     8701 ccctacccgt gtcgccgatc accggctgtt tgcccggact cgtgccccta aggtaccgtg
     8761 gtacgccaag cgaggggggc tcgtgactga acgcgaaaat gccgacgaga gcgccgagga
     8821 ttcagccggt gccggtgtcg cggttgcgtt gtccgcactg acgctggccg cgtgtggcgg
     8881 aggcgggggc agttctacgc ccccacccgt gcagaatccg ccgccggtcg tcccgcccgt
     8941 cgttccgccg cccgtcacca tcaccgacgc cgaggccgcg cgtttcctgc tgcaggccca
     9001 gttcgcggtc acggacacgg acctcgcggc ggtcaaagcc aacggctatg ccgcctggct
     9061 caccactaac tacaacgctg cgccgggaca gacgggcgtc gcctggctcg actcgcgcgg
     9121 ccacaactcc atcaccagcg aacagcgtta cttctggccg cagttcggcg acttcatgat
     9181 ctggaaccag ttgctcgccg ggcctgacca gatgcgcaag cgctttgcgt tcgccctgtc
     9241 ggaattcttc gtcgtgtccc tgagccccat cgacgggttc tacccgccct acgtgatcgc
     9301 cgcgtactgg gatgttttgt gcgcgaacgc gtacggcaat ttccgcacgc tgctcgaggc
     9361 gatcactctg aacgccggca tggggttttt cctcaacacc aagggcaacc tcaaggaaga
     9421 cgccaacggc cggcaacccg atgagaacta cgcgcgcgag gtcatgcagc tcttcaccat
     9481 cgggctgtac gagctcaatg ccgacggcac gcagcgcctc gacgccaaca accagccgat
     9541 cgagacctac ggccagagcg acatcaccaa tctggcgcgc gtgttcacgg gttatgactg
     9601 ggactacacc ggcaacgtca atacgaccgt cgcctggcac ccgtatccag tacccaacac
     9661 gcgcttcgcc acgaacccga tgcgcttcat cccgggcaat cattcaaacc tggcggcgac
     9721 cttcctcggc acgaccattc ccgccaacac gccgggtccc gaagcgttgc gcatcgcgct
     9781 cgatcgcctg ttcaatcacg ccaacaccgg cccgttcttc gcgcggcaga tgatccagcg
     9841 gctggtcacc agcaatccat cgcctgccta tgtgggccgc gtggccgctg catttgccga
     9901 caacggttcg ggcgtgcgcg gcgacatgaa ggcggtgtgg accgccatcc tcaacgacac
     9961 cgaggcgcgc acgttgcctg ccgcatcgga cacactgtcg ggcaagttgc gcgagccggt
    10021 ggtgcgcctc gtgcaatggg cgcgcaccgt gggcatcacg tcctcgaacg gcgcatacga
    10081 gatatacgac ctctcgggca gcgatacctc gctcggtcag agcccgctgc gttcaccgtc
    10141 ggtattcaat ttctttcgcc cgggttatgt gccacccaat acggcaatcg ccgcggccgg
    10201 caaacaggcg ccggaattcc agctgctgaa tgaaacgact accgcgggtt acatcaactt
    10261 catgcaggga atgacgcgca acggttacta cagcttcgat gtgaacccga gttacaccac
    10321 gttgatgacc attgcgcacg atgtacctgc cgtgatcgcc tggctgaatc tgcggctcac
    10381 tgcgaatcag ctgtcggcca cgactctggc agtgctgcag gccacgctga gcgcgttcaa
    10441 cgtgaccgcc ggcagcgcgg aaaacctcaa gctcgacatg ctcgccaccg cctgttttct
    10501 catcctgtgc tcacctgaat acctggtgca gaaatgaagc gccctgacgt aaacgccccc
    10561 gggttgtgga cgcggcgcgt gctcctgcag cgcgcgctgc acctgggtgc gatgggtgtg
    10621 gccacgccgc tcgccatcaa cctcgccgcc atcggcgaag ctgcggcctt cgacaacacc
    10681 gactacaagg cgctggtctg catctttctt tacggcggca acgactacgc caatacggtc
    10741 gtaccgtacg atgcgaccaa ctacgcgctg tatcaccaga ttcgcgccgg tgctgtcggc
    10801 gaaaaccagg cgggcatcgc actcgcgcga gcggcgctag agccgactgc gctcacgccg
    10861 aacaacggcc aggtgcttac agacaatctt cagtacgcgc tggcaccaca gctgacgggg
    10921 ctgaaatcca tctgggacgc gggacggctc gcggtgcaac tcaacgtcgg accgctcgtc
    10981 cagccgacga cgctggcgca gtaccagagc acgaatcgcg tggcgaatcc gctgccgccg
    11041 aaattgttct cgcacaacga tcagcaatcc gtatggcagt cgaacggaac ggaaggctcg
    11101 acggtgggct ggggtggcaa gctcggcgac atcgccatgt cgagcaacgc caccaactcg
    11161 ctgctcacct gcatctccgc gtcgggcaac gccgtgttcg tcgcgggacg cgatgcattg
    11221 caataccagg tgagccctgg cggcgcagtg cggatcaacg cattgaacac gccgctgcgg
    11281 gatgcgctga atgccatcat cacgcgccag agcagccacg tgctcgaaaa cgaatacgcc
    11341 atcgtcacgc ggcggtcgat ggacatggag ggcgtggtca acaccgcgct ctcgggcgtg
    11401 aacccgacga ctccgtttgg caccggcaat gggctcgcga accagctcaa gatcgtggcg
    11461 aggctcatcg gcgcgcgcgc cacgctcggg atgcgccggc aggtgttctt cgtgtcgctc
    11521 ggcggtttcg acaatcacaa cctgctcatg caggaccatc cgaatctcat gacgcgcgtg
    11581 aacgaggcga tgagcgcgtt ctatgcggcc accgtcgagc tgggtgtggc tgagaaagtg
    11641 actacgttca ccgcctcgga tttcgggcgc acgttgagtt ccaacgcgga tggttcggat
    11701 catggctggg gcagccatca cttcgtcatg ggcggcgccg tgaatggcgg gcggttctac
    11761 ggcaccgcgc cgcacgtctc cattcagacc gacgaccagg tggggcaggg gcgcctgtta
    11821 ccgagcacgt ccgtcgatga gttcgctgca acgctggcgc gctggttcgg ctgcaacgcc
    11881 ggcgagttgc ccggcatcct gcccaacgtc ggcaacttca ccaacaccga cctgggcttc
    11941 gtgtagatcg gctgaatcgg atttcgtata cgatgtggcc atgaacgacc acgacctgag
    12001 ccggcgcgat ttcctcgccg ccgccggtgg caccgccgca atggcgcttg ctcccgcctc
    12061 gacgattgcg gcggccggtt ccgcgaagcc gctcaatctc gccatcgtcg cggccccgag
    12121 cggctcgtat gtgtccgcgg acacgcgcct cacctcgctg aacagcggcg caacgcccgg
    12181 cagctccgcg gatccggcgc aactcgcctt cggcaccgcg cccgagaagg gcacgcaatg
    12241 ggcgcaatac gattggaacg agcccatctc gacgaacgcc atcgacgtgt actggtgggg
    12301 caagcactgg ggcattgcat tgccggtggc gagccgattg ctctactggg atggcgagca
    12361 gttcgttccg gtgaagaacg cgcgcgggtt cggtctcgag ccgaacgcct tcaatcgtac
    12421 tgaattcgat gccgtcacga cgcagcgtct gcgcctcgag atcgatccgg cagccgacaa
    12481 atctgtcgga gtcctgcagt ggcgcgtgtg gagcgtcggc gacgtgccga agttcgcacc
    12541 ggtggtgacg gccggcgtgg accgtaccgt catgctcggc gcgcctacct atctactggg
    12601 tcgcgccgcg tggctcgatc gcaagcgcgg cgacgccgcg cgctggcgca agatttcggg
    12661 gccgggcgtc gtgacgttcg ccgatgcgga cgccgcgcag accgcggcgc aggtttccgc
    12721 gccgggcgac tacaccttcg agctcagcgc gcagggcgat ggcttcacga ccaggtcgac
    12781 gctgcgactg cgcgccgaaa tgccgccacc gcgggaccgt ctcgatgtgg tctacaccac
    12841 gccgtatgcc atcctgagcc cgttgtggaa ggcgcgcgcg aaggctctca tcgtcaactg
    12901 gattccgcat tgcgtgcgct actgcgagcg caccgacctc aagatcggcg aggggggtct
    12961 agacaacttc atcgaagccg cgaaagcgtt gcgcggcgag ccacacggca ggcacaaggg
    13021 ctacgtgttc tcgaacgcgt ggatccacca gaccatcgag tcgatctgca tcgcactgat
    13081 ggtcgatgcg cagggcgacc aggaaatcct tgcggcgcag gcggggatgc gcacgacgct
    13141 cgagaagtgg attccgatcg tgctcgcggc gcaggagccc gatggttacc tgcaaaccgc
    13201 gtacacgctc gcggatcgtg ccaagtggcc gtcgcgctgg aatccgggga atcgcggcga
    13261 tcacgaaggg tacgtcgcgg gatacttcat cgagtccgcg atcaatcact acacgctcac
    13321 cgggggcaag gacctgcggc tctacatcgc cgcgaagaaa ctcgccgatt gctgggtggc
    13381 gaacctcggt cccgggaaaa aggtgtggtt cgacggacac caggagatgg agcaggcgct
    13441 cgtgcgcttc ggccggttcg tgaatgacat ggaaggcccg cttccgggaa caaacaaggg
    13501 caagggcgat gcgtacatcg cgttggcgcg gtttctgctc gaatcgcgcc gcggcggttc
    13561 ggaatacgac cagagccacc tgccgccgca gcagcagtac gaggccgtgg gccatgcggt
    13621 acgcgcggtg tatttctact ctggcatggc ggacatcgcg gcggaaacgc acgaccgcga
    13681 ctaccaaagc gcggtgcgct cgctcgccga caacatcgtg aatcgcaagt actacgtaac
    13741 gggcggcgtg ggcagtggcg acaccgcgga gggctttggc ggcgactatg tgctcggcaa
    13801 cgacgcctat tgcgaatcct gctcgagctg cggactcgtg ttcttcgact acaagctaaa
    13861 tctcgcgtac cacgacgcgc gctacgccga tctctacgag gagacgatct acaacgcgct
    13921 gcttggcgca accgacatcg agggacgaaa cttctgctat acgaatccgc tcgtaaacac
    13981 cgagcgtgcg gcgtggcatg tttgtccgtg ctgcgtaggc aacatcccgc gcacgctgct
    14041 catgctgccc acgtggtcgt acgtgaaaga tcgtgcaagt gtgtacgtga atctcttcgt
    14101 cggcagccgc atcaacgtgg gtgaggtggc gggcacgccg gtcgagatgg tccaggaaac
    14161 cgattacccg tggaaaggcg cggtagcgat caccgtcaat ccgcgcgatg cgcgcacgtt
    14221 ttccgtcaag gttcgcgtgc ccgatcgtgc gacgagtgcg ctgtactcgg ccacgccgtc
    14281 cgtgaagggc ctcggcaagc tgcgcgtgaa cggcgcgccg ctcgaggtgc gcatcgagca
    14341 aggctacgcc gtgatcacgc gcgagtggcg ccgcggcgat cgaatcgact tcgagctgcc
    14401 gctcacgccg cagcgaatca ccgcggacga gcgcgttgca gccacgcgcg gcaaggtggc
    14461 gctgcgctac gggccgttgc tatacaacgt cgagcttgcg gaccagccgc gcctggatcg
    14521 cgcgctgggg caagcgccgc tcaccgcgga gtggcgcgga gatttgttag gcggcgtaat
    14581 cacgctcaag gggaagtggg acgacggctc ggcactcacg gccgtgccaa acttcgtgcg
    14641 catgaatcgc ggcgagattc cagcgcgcga gtatccgacg gagaagccgc tcgacaaggt
    14701 gtactcgcag gtgtggatga aggcctgatg atgcatcttc agtttccgta caggaagggc
    14761 gcgccatact catccatcgc agccttccct gtggagatcc cggtgcgctc actaacaaca
    14821 atcaagacat tccgcggtgc aatgacgctg atactcgtcc ctgcgttgct ggcgctcggt
    14881 ggctgcgcca cggccgatgt agccatgaaa ctcgcgccgg atctggcggc gacgaccgcg
    14941 ttgccggtga cgggccggga cccggggact caactcgctt tcgggcgctg gcacgtcgcg
    15001 atgccggccc cgtaccggat ggatggctgg caaatctcgg gcacgccgcg attcgtgccc
    15061 gccaactccg agctatcggt caaggctgag cgtgcatcgc tcgaattcga ggtcgtggcg
    15121 gacgccggga gtcgccccgc ctcggccggc tgcctcgcgc agggcaggtt tgcgagcctc
    15181 accacttttc acgcgcgctc ggaagacgaa acttccgtca cgattccggg tttcccgcgg
    15241 ctcgactgcg agttctcggg cgcacgcacc ggcacgctcg cgctgcgtgc ggacttcgcc
    15301 actcaacgcg attggggcga cgccaggttc ggcgccgacc actggctcgt gcgctcggtg
    15361 aacacgcacg cgaagcagcg ttccaacttc ccgctgactc ggtttggcta cgaaatcgtg
    15421 ctcggcgaac gcgttgtcgc cgcggtggaa acctacggcg ccgggcgtgt ctgggtactg
    15481 ccggaccttt cgcgcgagca gcaggagcag ctgtcgaccg tcgccgcggc gctgctctac
    15541 tacggcaggc tgctggacct gcaggaataa tggtgtcagg caccgtttcc taggaatcgg
    15601 tgcctgacac catttatcag tcgatcttgg tgaagttgat cgtccggtcc ttgtagcgcg
    15661 tggcggccca cttggcggca tcttcggtca gagtcatcaa ctgctcgacc tcgatgagca
    15721 cggcattgtg atatgcggcg agcagctggc gattggccga gctcttcgcg gcgctgccgt
    15781 attgcgcagg gccgaggtcg gcttgagcgg cggtgatggc ggcgaggcgt gacttgcgcg
    15841 cggcttccac ctgctgggca atggccggca gcgccttgtt gtactcggcg atgatctgat
    15901 cgaacgcggc cttcagcgtc actttgtccg ctgcctggtc ggccgcggac gcgcatccac
    15961 cttcgccgtc gctgcacttg agcgacttgc cggccttgct cgcaatctcg gcctcgcgcg
    16021 cgcgaactcg ctgctggata tcggcgactg cggcgtactg cgcctggatg ccgcgcatcg
    16081 tcgacggatt cgcctggtaa tccgcgtagt gatcggccgc agcggtcacg gcttccggat
    16141 cggcggccat ggccttcacg tccttcagcg cgttctgctg ggttgcctgc tgcatctgca
    16201 tggcgagctt catcttttct tcctggctca tggccgcaat ttgcgccttc acctgctcgg
    16261 cgtaagccgg atcccgctgg gcacgctgga agtcgatgac gccaccggtt tgcgcggctg
    16321 tgggttgggc cgcggcgccg gcttcggtgc tgagcttctc gatgaaggtg cgctgatcct
    16381 tgagctgctt gcgcagcgcg acgagttccg ggctggcgag ccaggcaggg gccgcggcgg
    16441 ccgtggccgg gggtgccggc acgcgcgcat gcatggcctc gagggattgc tgggccgagg
    16501 cggcgctcgc gagcatcagg ccggccgcga gggcgaagaa cttcaacatg aacagaactc
    16561 ccggtaattc agtggatttc gaagggcgcg gagtatgggg aacgccctcg gctcgcggaa
    16621 gtgaccggtg tcaacgcagt tatgaaaaat gagactttag ttgtggttgg gtttgcgggc
    16681 ccctgccttg ggctatcctc tggtgcttcc tgagggggaa aaacatgccg ctgacacgct
    16741 ggacttcgat ccgtttcgtt tatgggctgg ccctggccat gttgtttgct agcccggtgc
    16801 ctgcttccga gcagccgccc acgctcgcca taggcgccgc catgccgcca ttctcgctgc
    16861 ccggcatcga tggcaagact tacaccgaga agagctttgc cagcgccgac attctcgtca
    16921 tggtgttcac ctgcgcgcat tgccccacgg cacaggccta ccaggagcgc atcaagaagc
    16981 tggtgaccga ctacgcttcg aagaacgtca cgctgatcgc catcaatccg aatcacgccg
    17041 acgccgtgcg cctcgatgag ctcgcgtact cggacctctc ggactcgttc gaggaaatgg
    17101 tggagcgcgc caaacaggag aagttcaact tcgtgtggct cgacgacggc ccgaagcagg
    17161 agatgtcaca cgcctatggc cccgtcgcca cgcctcacgt gttcatcttc gacaaggcgc
    17221 gaaagctccg cttcgaaggg cgcatcgacg actccgagcg tgaatcgtcg gccaccaaac
    17281 acgacacgcg cgcggcgttg gacgcgctgt tgaagggcaa agcgccgccg gtagtcacga
    17341 ccaaagtgtt cggttgttcg gtgaagtggg ccgacaaggt gcccgactac gccaactaca
    17401 ggaagaagtg ggccgcggaa ccggtgacgc tggaaaaagc cgatgccgcc atgctcaagg
    17461 ccattcgcga gaacaaggac tcggggaaaa tccgcttcat caatgtgtgg gccacctggt
    17521 gtggtccatg catcaccgag ttcgacgaac tggtggagca caacctgcga tttcgcatcc
    17581 gcggattcga gatggtgacc attgcggcgg agtttcccga ccaggaaccg caggtgcgcg
    17641 acttcctcac caagaagcat gcgtcgatga agaactacat ctacgccacc aaggacaacg
    17701 atgcgatgag cgaggccctg gatccggcct ggaagggccc gttgccttac accatcatcg
    17761 tcgacgagaa gggcaacgtg atctaccgcg agatgggctc catcaacttc cttgcgatgc
    17821 gccgcgccat cgtcccggcg ctgaatcgcc tgacaccttg gcagggcctc tcacccatca
    17881 agccttgaat ggtccctgga atggtgccag gcaccaattc ctggaaatcg gtgcctggca
    17941 ccgtttccgg agcgtaagaa acgccacgtc cgttcgtcat ctgtctgaac ccagacagga
    18001 ggcgaaagtg tgcgtacccc tatccttgca agtgcggcgg ccgtgagcgg cgctgccggc
    18061 gaagacatcg ctgcgctcgc gcggcaatac ggccccgtgg tttttcgcgc tgcgtggcgc
    18121 gtgctgggcg gccgcgctgc ggcggaggac gtgcagcaac aggtattcct gcggctcatc
    18181 gaatccccac ccggaaaggt ggaatcctgg ccggcgtacc tgacgaccgt cgccacgcgc
    18241 ctctccatcg accatctgcg ccggcaaagg cgctggaagt tattagcgcc gttgtggaaa
    18301 tcggatgagc cagagcccga tgactccccg gccgaggacg ccgagcgcgc cgaagccagc
    18361 cggttcatgc gcgccgcgct ggcgaggctc aatccgcgcg acgcatcgtg tttcgtgatg
    18421 cgccacctgc atggtctcgc gcccgctgcg atcggcgcgg cgctgggcct ttccgaaaat
    18481 cacgtcagcg tcagtgtgca ccgcgcgcgc aaggcgctcg aagccatcgc tgccaaggag
    18541 ctatcccgat gaactctctc gaccggaccc tggaattgtt cgccatgcgc gagcccgatc
    18601 tggcgctggt gaatggcgcg caacacaaac tcgaggcgct ggttgccgcg cgtgtttcga
    18661 cggctgccgc gcggcggccg gtgcgcgccg cgcgcggctg gctcgcggcc gcggccaccg
    18721 ccgctgtggt ggcagtggcg atgctgtggc tgccgttcgg atccgcaccg gtgctggctt
    18781 tcgcgaagat ccaggagcac ttccgcgact tccgcacgct gcgcttcgac gtcgagcagc
    18841 gcatggacgg ccggctcgtg atggagtcgc gcgtcagcgt gacgtacgac ggcaacgtgc
    18901 gcaccgatgt cggcgacgac gtcaccgtga tcgtgaactc cacggagcgg cgggtgctca
    18961 cgctgatgca tcgctcgcac gtggccatac cgtcacc
//
LOCUS       DASQJS010000139         7235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_385256, whole genome shotgun sequence.
ACCESSION   DASQJS010000139 DASQJS010000000
VERSION     DASQJS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385256"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..698)
                     /locus_tag="VEW08_05760"
     CDS             complement(21..698)
                     /locus_tag="VEW08_05760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYJ40276.1"
                     /translation="MTEKITTLAANIDARLPGLLTRITSLPDELCYEVQPENLKEVCL
                     SLRDSPELKFEILIDVAGIDYLDYGTTEWKTYSATASGFSRGVNRDAPRKPHDGARFA
                     VAYHLLSITHNQRVRLRSRCVDAEDPIIDSVVDIWPGANWFEREAFDLFGILFTGHPD
                     LRRILTDYGFIGHPFRKDFPLIGNVEVRYDADKQRVVYEPVSIELRTLVPKVIRDDNR
                     YAPVLKK"
     gene            complement(695..1219)
                     /locus_tag="VEW08_05765"
     CDS             complement(695..1219)
                     /locus_tag="VEW08_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HYJ40277.1"
                     /translation="MGLENQGVDENAAAGEFAERGFVTTTVDTVMNWARTGSLWPMTF
                     GLACCAVEMMHAGASRYDLDRFGVVFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMP
                     EPRWVISMGSCANGGGYYHYSYAVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQKK
                     IERTSSIANIPKRA"
     gene            complement(1210..1578)
                     /gene="ndhC"
                     /locus_tag="VEW08_05770"
     CDS             complement(1210..1578)
                     /gene="ndhC"
                     /locus_tag="VEW08_05770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HYJ40278.1"
                     /translation="MLANYLPVLIFLTVATGLALLLMTLGLIIGRWQAKARNDAQKLS
                     PYECGFEAFEDSRMKFDVRYYLVAILFIVFDLEIAFLFPWAVVLDSIGTFGLVSMGIF
                     LFILVVGFIYEWKKGALEWD"
     gene            complement(1696..1780)
                     /locus_tag="VEW08_05775"
     tRNA            complement(1696..1780)
                     /locus_tag="VEW08_05775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1744..1746),aa:Leu,seq:gag)
     gene            complement(1821..2201)
                     /gene="secG"
                     /locus_tag="VEW08_05780"
     CDS             complement(1821..2201)
                     /gene="secG"
                     /locus_tag="VEW08_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HYJ40279.1"
                     /translation="MLRTILTILQVLSAFAIVGLVLLQRGKGADAGAGFGAGASGTVF
                     GARGATTGLSRMTAIFAAIFMLNSLCLTYLFQRDARVQPTSLLDKVPVSTPAPAPAPP
                     PVDANAVPVPAAPVEQATPEPAKP"
     gene            complement(2217..2969)
                     /gene="tpiA"
                     /locus_tag="VEW08_05785"
     CDS             complement(2217..2969)
                     /gene="tpiA"
                     /locus_tag="VEW08_05785"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HYJ40280.1"
                     /translation="MRRQIIAGNWKMHGSRAENAALVESIVHKLPNSSAEVWVCPPFV
                     YLAELSRALAGSRISLGSQDVCAETQGAFTGEVSAAMLKDVGCAGAIVGHSERRALFG
                     ETDQLVARKFAAVLAAGLTPILCVGELLAEREAGRTFEVVDRQLDAVLDLSGVAAFSR
                     AVIAYEPVWAIGTGKTASPQQAEEVHAHIRGRIAGRDAKIAALVRIQYGGSVKAANAA
                     ELFSMPNVDGGLIGGASLKAEEFLAIAAAARA"
     gene            complement(3036..4370)
                     /gene="glmM"
                     /locus_tag="VEW08_05790"
     CDS             complement(3036..4370)
                     /gene="glmM"
                     /locus_tag="VEW08_05790"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004639966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HYJ40281.1"
                     /translation="MARKYFGTDGIRGRVGEHPMTVDFALRLASAAARVLAPNGGTVL
                     IGKDTRLSGYMFEAALEAGFVAAGVNVMLIGPLPTPGIAFLTRKFECSFGIVISASHN
                     LYDDNGIKFFDADGGKLNDEIEEAIEAELDSGPITRSSVALGRASRVDKSRRHYQDFC
                     RESLPKGMTLEGMKLVVDCANGAGYKVVPRTLTDLGAEIIPIGCSPNGRNINDGCGST
                     APSLLQLTVPGVRAAAGIALDGDGDRLVMVDGLGRIVDGDQLIYIIATARKRAGTLRG
                     PVVGTVMSNLGLEVALREQGIEFKRAQVGDRYVLAMLRENGGSLGGESSGHILLLDKT
                     TTGDALMAGLQVLAVMRETGQGLAELAAGMSRFPQTMINVKVAKRFDPKTIPEVEDTV
                     QKVSLRLGGEGRVVLRPSGTEPVIRVMVEARDETDTVSCAEEIAAVVRRVAS"
     gene            complement(4360..5217)
                     /gene="folP"
                     /locus_tag="VEW08_05795"
     CDS             complement(4360..5217)
                     /gene="folP"
                     /locus_tag="VEW08_05795"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HYJ40282.1"
                     /translation="MSTVARWQCRDRVLDLGAPVVMGVLNVTPDSFSDGGRYSELDSA
                     LRHACRMIEEGAAIVDVGGESTRPGAAAATLDDELARVIPVIEAVRRESTAFISVDTS
                     KPEVMRAACDAGADIINDVRALSAPGALAAAASTRAGICLMHMQGEPRTMQQAPRYSD
                     VVGEVAAYLTARVTACREAGIVAARLAVDPGFGFGKRVVDNLALLKHLARFRELGVAL
                     AVGLSRKSMLATLTGRDVGDRTAGSVALAAIAVLNGARIVRAHDVAATVDAIRVAAAV
                     SQGENFDGT"
     gene            complement(5255..7171)
                     /gene="ftsH"
                     /locus_tag="VEW08_05800"
     CDS             complement(5255..7171)
                     /gene="ftsH"
                     /locus_tag="VEW08_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HYJ40283.1"
                     /translation="MNDLTKNILLWVVIVTVMVVVFSRYVPSGAEPTQVSYSTVLDDV
                     RNGRVDSVTLQGNEILGLRKDKTKFRAYNPETDNTVLIGTLDKARVDFKGEPPKQQNF
                     LVQLLLQLAPALLLILVFVYMLRQMQGSAGGRGAMSFGKSRARLLGEDQVNVTFADVA
                     GVDEAKQEVGEIVDFLKDPSKFQKLGGKIPKGVLMVGSPGTGKTLLARAIAGEAKVPF
                     FTISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGMGGGHDER
                     EQTLNQLLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDVRGREQ
                     ILRVHMRKVPLGEDVKPAYIARGTPGFSGADLANLVNEAALFAARANKRTVSMDELER
                     AKDKIMMGAERRSMVMSEKEKLNTAYHEAGHAIVGVNLPEHDPVYKVTIIPRGRALGV
                     TFFLPEEDRYSYSKQRLESSIASLFGGRIAEELAFGPAAVTTGASNDIERATMLARNM
                     VTKWGLSDKLGPLTYAEESGEIFLGRQVTQHKQVSDETAHVIDVEVRRVIDTAYRKAQ
                     NILETNRDKLDAMASALIKYETIDEQQIKDIMAGRAPKPPADWDDSIGTPPPKRSEGE
                     PEGGSTIGSPAGQH"
BASE COUNT         1346 a         2321 c         2272 g         1296 t
ORIGIN      
        1 cgtgaaattg cggatctcgt tcatttcttc agcaccggcg cgtagcggtt gtcgtcgcgg
       61 atgactttcg gcaccagcgt gcgcagctcg atcgacacgg gctcgtacac cacgcgctgc
      121 ttgtcggcgt cgtagcgcac ttcgacgttg ccgatgagcg ggaaatcctt gcggaacgga
      181 tgcccgatga agccgtagtc cgtgaggatg cggcgcagat ccgggtgacc ggtgaacagg
      241 atgccgaaca ggtcgaacgc ctcgcgctcg aaccagttgg cgccgggcca gatgtcgacc
      301 acggaatcga tgatgggatc ttcggcatcg acacagcgcg aacgcaggcg cacgcgctga
      361 ttgtgcgtga tcgacagcag gtgatacgcg acggcgaaac gcgcgccgtc atgcggcttg
      421 cgcggtgcgt cgcgattcac gccgcggctg aaccccgaag ccgtggccga atacgtcttc
      481 cactcggtcg tgccgtaatc gagatagtcg atgccagcga cgtcgatgag gatttcgaac
      541 ttgagctcgg gcgagtcccg caggctcagg cacacttctt tcaggttctc cggctgcact
      601 tcgtagcaca actcatcggg gagcgacgtt atgcgcgtga gcaagcccgg caggcgggcg
      661 tcgatattcg cggcaagggt cgtgatcttt tcggtcatgc gcgcttcggg atattcgcga
      721 tcgagctcgt gcgctcgatc ttcttctgca gctggatgat gccgtacagc agcgcttcgg
      781 ccgttggcgg acagccggga acatagacat ccacggggac gatgcggtcg cagccacgaa
      841 cgaccgcata ggaatagtgg tagtagccgc caccattcgc gcaggagccc atggagatga
      901 cccagcgcgg ctcgggcatc tggtcgtaga ccttgcgcaa cgccggcgcc atcttgttga
      961 ccagcgtgcc ggcgacgatc atgacgtctg actgtcgcgg actcggacga aataccacgc
     1021 cgaaccggtc gaggtcatat cgcgacgcgc cggcatgcat catttcgacg gcgcagcacg
     1081 ccagaccgaa agtcatgggc cacagcgaac cggtgcgcgc ccaattcatg acggtgtcga
     1141 cggtggtcgt gacgaaaccg cgctcggcga attcgcccgc ggcggcgttt tcgtctacac
     1201 cctggttctc taatcccatt cgagcgctcc cttcttccac tcgtagatga atccgaccac
     1261 gaggatgaac aggaagatgc ccatggacac gaggccgaag gtaccgatgc tgtcgagcac
     1321 cacggcccac ggaaacagga atgcgatttc gagatcgaac acgatgaaga ggatggcgac
     1381 gaggtagtaa cgcacgtcga atttcatgcg gctgtcttcg aaagcttcga agccgcattc
     1441 atagggggag agtttctgcg cgtcgtttct ggctttcgcc tgccagcggc cgatgatcag
     1501 gcccagcgtc atcagaagga gcgcaagccc cgtcgcgacg gtgaggaaaa tcagaacggg
     1561 cagatagttg gcaagcatct gttgagccct aaaccgtcat cgagaaccat cgcgaaaagg
     1621 ggcagcaacc tctcaacgat gagagacaac cgcccccgga aaattcgggc gcgattgtaa
     1681 cagggttaac aaggatggtg ccgacgggga gactcgaact cccacggatc gctccgctag
     1741 cccctcaagc tagtgtgtct accaattcca ccacgtcggc gcccaggaaa ttttttaagg
     1801 tcaaatcttc aaggcttaat tcaaggcttc gccggttccg gggtcgcctg ttccaccgga
     1861 gctgcgggca cgggcaccgc attcgcatcc accggcggcg gtgctggcgc gggcgccggc
     1921 gtgctcaccg gcaccttgtc cagcagcgag gtcggttgca cccgggcatc gcgctggaag
     1981 agataagtga ggcacaggct gttcagcatg aaaatcgcgg caaatatcgc ggtcatgcgc
     2041 gacaggccgg tagtcgcgcc acgcgcgccg aacaccgtac ccgaagcacc ggctccgaag
     2101 cccgcgccgg catccgcacc cttgcctcgc tgcaggagca cgagcccaac gatggcgaat
     2161 gccgaaagaa cctgcagaat tgtgagaatc gttcgcaaca tctatgtgta cctaaatcag
     2221 gcgcgcgccg cggcggcaat cgcgaggaat tcttcggcct tgagcgaggc gccgccgatg
     2281 agcccaccgt ccacgttcgg catcgagaag agctccgcgg cgttcgcggc cttcacgctg
     2341 ccaccgtact ggatgcgcac gagggcggcg atcttagcat cgcgacctgc gattcgcccg
     2401 cggatgtggg catgcacttc ctcggcctgt tgcgggctcg ccgtcttgcc ggtaccgatg
     2461 gcccacacgg gctcgtaggc gatgacggca cgcgagaacg cggcgacgcc ggacagatcg
     2521 agcacggcgt ccagctgacg gtccaccacc tcgaacgtgc ggccggcttc gcgttcggcg
     2581 agcagttcgc ccacgcacag aatgggggtc agtcccgcgg cgagcacagc agcaaatttt
     2641 cgcgccacca gctggtcggt ctcgccgaac agcgcgcgcc tttcggaatg gccaacgatc
     2701 gcgccggcgc aacccacgtc tttgagcatg gcggcggaca cttcaccggt gaatgcaccc
     2761 tgggtttcgg cacacacgtc ctgggacccc aggctgatgc gcgaaccggc gagcgcacgg
     2821 gaaagctcgg ccaggtagac gaacggcgga cacacccaga cttccgccga cgagttcgga
     2881 agtttgtgca cgatggactc gaccagcgcc gcgttttcgg cgcgcgagcc gtgcattttc
     2941 cagttgcctg cgatgatctg gcggcgcatt tgtttagcca tttagtggcc gtttcggccg
     3001 ctcaaaaaag gggcgcgatg atagcggcgc gcccctcagg acgcaacgcg gcgaaccacg
     3061 gcggcgattt cctcggcaca gctgaccgta tcggtctcgt cgcgcgcctc gaccattacc
     3121 cggatcacgg gttcagtgcc ggatgggcgc aggaccacgc gcccctcgcc ccccaggcgc
     3181 aacgacacct tctgcacggt gtcttcgacc tcgggaatgg tcttcggatc gaaccgtttc
     3241 gccactttca cattgatcat ggtctgcggg aaacgtgaca ttcccgcggc gagttccgcc
     3301 aggccctgcc ccgtttcacg catgacggcc aggacctgaa gtcccgccat caacgcgtcg
     3361 cccgtggtgg tcttgtcgag cagcagaatg tgccccgaag actcgccacc caacgatccg
     3421 ccgttctcgc gcagcatggc cagcacgtag cggtcgccta cctgcgcgcg cttgaattca
     3481 atgccctgct cgcgcaacgc cacttcgagt ccgagattgc tcatgacggt tccgactacc
     3541 ggaccccgca gtgttccggc gcgcttgcgc gccgtcgcga tgatgtaaat gagctggtcg
     3601 ccgtcgacga tgcgcccgag cccatcgacc atgacaaggc ggtcgccatc accatcgagc
     3661 gcaatgccgg cggccgcgcg cacacccggc acggtgagct gcagcaggct cggcgcggtc
     3721 gagccgcagc cgtcgttgat gttccggcca ttcggcgaac agccgatggg gatgatctcg
     3781 gcgccgagat ccgtgagcgt gcgcggcacc actttgtagc cggcaccatt cgcgcaatcg
     3841 accaccagct tcatgccttc gagcgtcatg cccttgggca gcgactcgcg acagaagtcc
     3901 tgatagtggc ggcgcgactt gtcgacgcgc gaggcgcgac cgagggcaac cgatgaacgc
     3961 gtgatggggc cgctgtcgag ctcggcctcg atggcttctt cgatttcatc gttcagcttg
     4021 ccgccgtccg catcgaaaaa cttgatgccg ttgtcgtcgt aaagattgtg tgacgcgctg
     4081 atgacgatgc caaaactgca ttcgaatttt cgcgtgagga acgcgatgcc cggcgtgggc
     4141 agcggcccta tcagcatgac gttgacgccg gccgccacga agccagcctc gagtgccgcc
     4201 tcgaacatgt atccggaaag gcgcgtgtcc ttgccgatga gcacggtgcc accattgggc
     4261 gcgagcaccc gcgccgcggc gctggcgagc cgcaatgcga agtccaccgt catgggatgc
     4321 tcgccaaccc ggccacgaat tccgtcggtg ccgaaatact tacgtgccat cgaaattctc
     4381 tccttgcgat accgcggcgg ccacgcggat cgcgtcgacc gtggcagcca cgtcgtgggc
     4441 gcgcacgata cgcgctccgt tgagaaccgc aatggcggcc agtgcgacgc tgcccgcagt
     4501 tcgatcaccc acatcacggc cggtcagcgt ggcaagcatg gacttgcgcg acagacccac
     4561 cgccagcgca acgcccaact cgcggaatcg cgcgaggtgc ttgagcagcg caagattgtc
     4621 gaccacgcgt ttgccgaagc cgaaacccgg atcgacggct agccgcgcgg caacaatgcc
     4681 agcctcgcgg caggcggtga cacgcgcggt gagatacgca gccacttcgc ccaccacgtc
     4741 cgagtaccgc ggcgcctgct gcatggtccg tggctcgcct tgcatgtgca tgaggcagat
     4801 gccagcccgc gtgctcgcgg ccgccgccag cgccccaggg gcggacaagg cacgtacgtc
     4861 gttgatgatg tctgcgccgg catcacaggc cgcgcgcatg acttccggct tgctggtgtc
     4921 gaccgagatg aaagctgtgg attcgcgccg tacggcttcg atgaccggga tgacgcgcgc
     4981 caactcatca tcgagcgtcg cggcggcggc accgggccga gtggattcgc caccgacatc
     5041 gacgatggcc gcgccctcct cgatcatccg gcatgcatgg cgcagggcag agtcgagctc
     5101 ggaataccgg ccaccgtcag aaaaggaatc cggcgtgacg ttgagcacgc ccatcaccac
     5161 gggggcgccg agatcgagta ctcgatcccg gcattgccat cgcgcgaccg tgctcatggc
     5221 gaacgaccgc cggtactttc ccgactaact aatctcagtg ctgaccagcg ggcgaaccga
     5281 tggtggatcc accctcgggc tcgccttcag aacgcttcgg cggcggagtg ccgatcgaat
     5341 cgtcccaatc tgcaggcggc ttcggcgcgc gcccggccat gatgtccttg atctgctgtt
     5401 cgtcgatcgt ctcgtacttg atgagcgcag acgccatcgc atccagcttg tcgcgattgg
     5461 tctcgagaat gttctgcgcc ttgcggtacg cggtgtcgat gacacgacgg acttccacgt
     5521 cgatgacgtg cgcggtttcg tcggacacct gcttgtgctg cgtgacctga cggccgagga
     5581 atatctcgcc gctttcttcc gcgtaggtca acgggccgag tttgtcggac aggccccact
     5641 tcgtgaccat gttgcgggcg agcatggtgg cgcgctcgat atcgttcgac gcgccggtag
     5701 tcaccgccgc cgggccgaac gcgagctctt cggcgatgcg cccgccgaac aggctggcga
     5761 tcgacgattc gagtcgctgc ttgctgtagc tgtagcggtc ttcctcgggc aggaagaacg
     5821 tgacgcccag cgcgcggccg cgcggaatga tcgtgacctt atacaccggg tcgtgctcgg
     5881 ggaggttcac gcccacgatg gcgtgaccag cctcgtgata ggcggtgttg agcttctctt
     5941 tctcgctcat gaccatggag cgacgttcgg cgcccatcat gatcttgtcc ttggcgcgct
     6001 cgagttcgtc catggatacg gtgcgcttgt tggcgcgcgc ggcaaacaac gccgcttcgt
     6061 tcaccaggtt ggcgagatcc gcgcctgaaa atccgggcgt gccgcgtgcg atgtatgcgg
     6121 gcttcacgtc ttcgcccagc gggaccttgc gcatgtgcac gcgcaggatc tgctcacggc
     6181 cgcgcacgtc aggcagcggc accacgacct ggcggtcgaa gcggcccgga cgcagcagcg
     6241 ccggatcgag cacgtcggga cggttggtgg cggcgatgac gatgatgcct tcgttgcctt
     6301 cgaagccgtc catctcgacc agcaactggt tcagggtctg ttcgcgttcg tcgtgaccgc
     6361 cgcccatgcc tgcgccacga tgacgaccca ccgcgtcgat ttcatcgatg aagatgatgc
     6421 agggcgcgtg cttcttggcc tgctcgaaca tgtcgcgcac gcgcgaggcg ccgacgccga
     6481 cgaacatttc gacgaagtcg gagccggaaa tcgtgaagaa cggcaccttg gcctcgccgg
     6541 cgatggcgcg cgcgagcagc gtcttgccgg tgccgggcga gcccaccatg agcacgccct
     6601 tcggaatctt gccgcctaac ttctggaact tggatgggtc cttgaggaag tcgacgatct
     6661 cgcccacttc ctgcttggct tcgtccacgc ccgcgacatc cgcaaaggtg acgttgacct
     6721 ggtcctcgcc gagcagccgg gcgcggctct tgccgaacga catggcgccg cggccaccgg
     6781 ccgagccctg catctgccgc agcatgtaca cgaacacgag gatgagcagc agcgccggcg
     6841 cgagctgcaa cagcaactgc acgaggaaat tctgctgctt cggtggttcg cccttgaagt
     6901 cgacgcgcgc cttgtccagc gtgccgatga gcaccgtgtt gtccgtttcg gggttgtaag
     6961 cgcggaactt cgtcttgtcc ttgcgaagcc cgaggatctc gttgccttgc agagtgaccg
     7021 aatcgacgcg gccatttcgc acgtcatcga gtacggtcga atatgaaacc tgcgtgggtt
     7081 cggcaccgct ggggacgtag cggctgaaca ccaccaccat caccgtcacg ataaccaccc
     7141 agagcaggat atttttcgtc agatcattca aaggcttacc ctcgcaaggc gaccggcctg
     7201 attaaggagt gaggtcacgc tacaccaatc gaaaa
//
LOCUS       DASQJS010000140         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2585256, whole genome shotgun sequence.
ACCESSION   DASQJS010000140 DASQJS010000000
VERSION     DASQJS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2585256"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1134
                     /gene="mutY"
                     /locus_tag="VEW08_05805"
     CDS             73..1134
                     /gene="mutY"
                     /locus_tag="VEW08_05805"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF008132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HYJ40284.1"
                     /translation="MNTRAKGIASRLLAWHSKKGRHDLPWQKDRSAYRVWVSEIMLQQ
                     TQVTTVIPYFDRFMRRFPDVRALANATVDEVMHLWTGLGYYARARNLHRAAQVIRDEH
                     RGIFPTGLDAVMALPGIGRSTAGAILAISAGSRHPILDGNVKRVLARYYAVDGAPEEK
                     ATLETLWRLADDNTPAKNVAVYTQAIMDLGATLCTRGKPRCDDCPIAIDCRARIEGRQ
                     GELPAAKRKRGARRQKHAVMLVARRATEVLLVQRPPSGIWGGLWCLPEFDRRDEAELY
                     AATTLASAKLARAPMPDIAHSFTHFDLVITPVVAKCHGETRVHDGTALWYDLTRPARV
                     GLPAPIKALLGTLLERSES"
     gene            1131..1400
                     /locus_tag="VEW08_05810"
     CDS             1131..1400
                     /locus_tag="VEW08_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HYJ40285.1"
                     /translation="MTRMVNCVLLKKEAPGLDRPTYPGELGKRVFENVSKEAWQQWVK
                     HQVMLINEYRLTPIEPAHRKRLEFEMEKFFFGEGSAAPKEYVKPD"
     gene            complement(1408..1899)
                     /locus_tag="VEW08_05815"
     CDS             complement(1408..1899)
                     /locus_tag="VEW08_05815"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYJ40286.1"
                     /translation="MRSRLAFLLLLLPFWHTPSFAQCAPAPDSPYFFRNLAEQRAEAK
                     LASDRGFYEGLLSEHFAGKGHDGKALPRQEFIESELAVKRDSTRKGFYAVRNFSLVEH
                     RKGLAITSYLLVEGDIGEGNSNARETWQREVFEVQDGKWRLVSIEDTEPRPAQQIQDS
                     PGG"
BASE COUNT          393 a          620 c          662 g          362 t
ORIGIN      
        1 agatgtaacg cttcggctgt tgctcgacga gcagggtctg cgcagctcac caaaaccacc
       61 cgcggtgacg ccatgaacac gcgagcaaag ggaattgcct cgcgcctgct ggcgtggcat
      121 tcaaaaaaag gtcgtcacga cctgccgtgg cagaaagatc gcagcgcgta tcgcgtgtgg
      181 gtcagcgaaa tcatgttgca gcagactcag gtgaccacgg tcatcccgta tttcgaccgc
      241 ttcatgcggc gtttccccga tgtgcgggcg ctggcgaacg ccaccgtgga tgaagtgatg
      301 cacttgtgga ccgggctcgg ctactacgcg cgagcgcgca atctgcatcg cgcggcgcag
      361 gtgattcgcg atgaacaccg cggaatattt cccacgggac tcgacgcggt gatggcgctg
      421 ccgggaatcg gccgctcgac ggccggggcc attctcgcga tctccgcagg cagccggcat
      481 cccatactcg atggcaacgt gaagcgggtt ctcgcgcgct actacgccgt cgatggcgcg
      541 cccgaagaaa aagccacgct cgaaactctc tggcgcctgg ccgacgacaa cacgccggcg
      601 aagaacgtgg cggtctacac gcaggccatc atggatctgg gagccacctt gtgcacgcgc
      661 ggcaagccgc gttgtgatga ctgtccgatt gccattgatt gccgagcacg catcgagggg
      721 cggcagggcg aactgccggc ggccaaacgc aagcggggtg cgcgcaggca gaaacacgcc
      781 gtgatgctgg tggcgcgtcg cgctacggag gtgctgctgg tgcagcgccc gccaagcgga
      841 atctgggggg gtctctggtg tctgcccgag ttcgatcgtc gagacgaggc ggaactgtat
      901 gccgccacca cgctcgcgag cgcgaagctg gcgcgtgcgc cgatgccaga catcgcgcac
      961 agcttcacgc atttcgacct ggtgatcact cccgtcgtcg cgaaatgcca cggcgagact
     1021 cgcgtgcacg acggcaccgc gctgtggtat gacctcacgc ggcccgcgcg tgtcgggctg
     1081 cccgcgccga tcaaggcgtt gctcggaacc ttgttagaga gaagtgaatc atgacccgga
     1141 tggtgaattg cgttctgttg aagaaagagg cgcccggtct cgaccggccg acctatccgg
     1201 gcgagctcgg caagcgagtc ttcgagaacg tgtcgaagga agcctggcag cagtgggtca
     1261 aacaccaggt catgctcatc aacgaatacc ggttgacacc gatcgaaccc gcgcaccgca
     1321 agcggctcga gttcgagatg gaaaaatttt tcttcggcga gggttcggcg gcgccgaagg
     1381 agtacgtgaa gccggactga tcgggcgtca accgcccggc gagtcctgaa tctgttgcgc
     1441 cggccgcggc tcggtgtcct cgatcgacac cagtcgccac ttcccgtcct gcacttcgaa
     1501 gacttcgcgt tgccaggtct cgcgagcgtt cgaattgcct tctccgatgt cgccttcgac
     1561 gagcaggtag ctggtgatgg cgaggccttt gcggtgctcg accaggctga agttcctgac
     1621 agcgtagaag cctttacgcg tggagtcgcg cttcaccgcc agctcactct cgatgaattc
     1681 ctgcctgggc agcgccttgc cgtcgtggcc tttgccggca aaatgttcgc tgagcaggcc
     1741 ttcatagaag ccgcgatcgg aagccagttt cgcttctgca cgctgctcgg cgaggttgcg
     1801 gaagaagtaa ggcgaatcgg gcgccggagc acattgcgca aatgacggcg tgtgccagaa
     1861 gggcagcaac aggagcagaa atgccaggcg cgaacgcatg gcacccatca tatgagagtg
     1921 gtgccgggcc gggaaaccgg gataactcgg cgtttgccag tcatccggtt tctcgatgcc
     1981 gagttatccc ggtttcccgg cccggcacca caagacgccc tagaacttgc cggcctg
//
LOCUS       DASQJS010000141        11437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3527049, whole genome shotgun sequence.
ACCESSION   DASQJS010000141 DASQJS010000000
VERSION     DASQJS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11437
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3527049"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2295)
                     /locus_tag="VEW08_05820"
     CDS             complement(<1..2295)
                     /locus_tag="VEW08_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ40287.1"
                     /translation="MSFRYSVRRAVRRALWAGAFASAVPVLPALAADPSSPAAEVVEE
                     VVVTGSRIRVRDYEAISPVTTVSADAIQATGQLSVEEVLNRLPQVVPGLTANSNNPAN
                     GTSTVDLRGIGTPRTLVLINGRRLTPSTQTGVVDLNNVPVRLIDRVEVVTGGASAVYG
                     SDALAGVVNFIMKEDYQGFDFGIQHGQSAQGDGAQSQFDVLLGGNLGEDRGNITVFAS
                     YFDRDSVLQSEREFTRVDRSGNGSPTGNARLPNVGLNPYPTVGDFLGGGSTSRDYAFN
                     LNNNGGVRVWNNTLPETSADGQGDKYNFSPVNFLLTPGERINIGAMGNLKITDKLTAY
                     MDIMYVDSRNAAQLAPTPAVNVPFDPDSPLLSAQARALMDARPNPNAPGFLTRRMTEI
                     GPRLQENKSKLQQADVGFRGDLPYKDWQFDVSYQFGRTDFINVTHNDVSRSKFAAGLS
                     NCPAEYVKFVPGCVPVNAFGVGSITPEMANFIRLDFSDSTIFERQLANASINGTLAEL
                     PAGPLGFALGAEYRKDSSEYVPDLAKKQGDILGFNAAQPVAGEFDVTELFAETVVPLL
                     KDLPAVKSLSLEAGLRYSDYSSVGEVTSYKAGLDWAPMSSLHVRGMYQRAVRAPSVFE
                     LFQAGDKGFPIVNDPCASTRANGTTRVVPADVAAFCQQTWGINAATYTQTNSQIEALF
                     YGNTNLSEETSDTLTFGFALTPEAIPDLHLSVDYYKIKVRDFVNTLEGGPTGVVLACF
                     ASLDIDSDACFSRDLNEALVFRDAV"
     gene            complement(2399..3289)
                     /locus_tag="VEW08_05825"
     CDS             complement(2399..3289)
                     /locus_tag="VEW08_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYJ40288.1"
                     /translation="MLLLRSLPELSAPESALSESFRERWSRESSIIWGRSRSAEFGPY
                     PQLLSIRAVWGGAQFCHVDGRTLAVDDDNFLILNPGRNCATSIHAADPVESLTICFAP
                     QPGGLATQEPEFLENLQPHDSAVSPVLRFIRAHLARGFVEEDWYEEQLVFLLERMRTR
                     QAKLHEQIDQIDLIRPATRREAFRRIAMATDYLHANYAQDVDLGTLARMAYLSKYHFL
                     RLFTLIHGITPRKFLQRKRVDVAVRLLESTRLPISEVTARVGFAFESTLLRHVRRRTK
                     LSPSQLRIRMCDAAASRTSA"
     gene            complement(3310..5898)
                     /locus_tag="VEW08_05830"
     CDS             complement(3310..5898)
                     /locus_tag="VEW08_05830"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HYJ40289.1"
                     /translation="MLVASLLLVSCGGSGGAGAPPPASYFVGFTVTGLVGSGLALRNG
                     VDTVSITANGQSRFPTALPDGAPFAVTVASQPAAPAQACTVSPDTGAVANADAAVAVR
                     CVTTAAPRFAYAFNFGSDSISIYNVDAATGQLRARGYVKTGAGGQRPVQDPAGKFSFV
                     LNSGIDRDPAAPLSFSSISVFAHDNVNGDLQEIDGSPFVTSHSLVGASALTVHPSGKF
                     VYVTTRDDAIFQWSVAPDGALTPINDDLVPAGNAPRELVFDARGRFAYLTHVDHGSDG
                     IYVYEADAATGALAERAGLRVQFTNNDLRTSNFVFHPGGKFAYVVNQDFAGNPGSLAA
                     FAVNATTGELTPLAGQPHDLPVQASGVPVFDVSGRFAYVMSFGTPAVRGAVAGFSVNL
                     TTGVLTPLPGSPYEAGFSPGSLSLTPSNEFLYVANRSRGPAEPAGTGSVTAFRVDRDT
                     GVLARIPGIDALQPAPFTASVDPGGRYLYAAGLSGDRIHAYSIGAAGELQPISRGAVI
                     RAGSQPTSIDAFVSPVVATPAVFLPRFLYMADTPGNSVTSFVIDPTTAPLRVSGSISL
                     PDTLPRALVASTDGKFLHVAGLAGSVTTLAIDNANGALTAIEAGTTPAGASPAAIVAD
                     PGNRFVYVGNFDEGTVTAYRRARNGGSLTAIGPPFATGPGPLSMVIDPTGRNLYVMSF
                     ARVRVFGLDSVTGEAHALPDEQGGLKELASALGTQAAFDPSGRWLYVVSPQGMLERFP
                     VDSRSGALGDGSVLGIDPGSLSLTIDPSGRFLYTAGSNSSTISAFSLDQRTGAPVEIG
                     RTPLVTEPRQVLADFSGNAIWVLSFDNSVASFGIDPLSGALTGISQQPQPANRATQLA
                     TVGVVR"
     gene            6117..6692
                     /locus_tag="VEW08_05835"
     CDS             6117..6692
                     /locus_tag="VEW08_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HYJ40290.1"
                     /translation="MSEIAFLRALEAGRLDDHQTLGDLFTSFYGELHRRAKWELRQNT
                     PLSLGPTTLLHETFLDMSNRESVSFTDHRKFIAYAARAMRGLIVNHFRNRHAQKRGGR
                     LEFTALHTDLPEDRQSIDIEGLGEALESLATLDPRLAECVDMKFFCGLSFSEIANLRN
                     VSERTVQRDWDKARLLLNQLMMVHDDRPKAG"
     gene            complement(6718..7728)
                     /locus_tag="VEW08_05840"
     CDS             complement(6718..7728)
                     /locus_tag="VEW08_05840"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HYJ40291.1"
                     /translation="MNSLNLPVCAFALLLAHAAVPQLHAAELTLPREGWASWQVSAAE
                     NAPAWCCWSSWKDRDVSPTSCRLDDRPDHYGTRSDEKTDSIKVYARVAGGRIDRLQVL
                     SATCQVETRTPVNELGNVTVEDSARWLIARARQSGNDAATGNPISESALAALAMHRGD
                     FARDALAEFSRDPRIETRKWAVFWLAQLRGDEGAAITSTVMFNDKDEDVRKHAAFALA
                     QTKSPRAAADLIRQGNTDKVGDVRAQAWFWLAQTGAAEAEQAIAAALRKDADEHVREH
                     AVFALSQLPDDRAPRALIATAEDQSLSREQRKRAVFWLSQSESDSAQAYLEKVLARNT
                     AN"
     gene            complement(7725..8519)
                     /locus_tag="VEW08_05845"
     CDS             complement(7725..8519)
                     /locus_tag="VEW08_05845"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HYJ40292.1"
                     /translation="MAQPAERALPIIKKVLAGSQTTLVKQRALFVLSQIGTPEAQAIL
                     AQTSRSSDAALRGEAIRSIGIGGDPKALDALQEVYNAGDPDVKRDVLQAWMIAGRKEA
                     VYQAALNGKSEEESNEAIRMLAVMGATDELRKLGDRPNAASGLVDAFAISGDLASLRK
                     IAEGSGDRSLRLDAIRKIGMVQGDGARAALRDIYSRSTDEEIKEAALQGMLMSGDEQG
                     VLTLYRGAKASNEKRMLLRYLSMMDGDAALQAIDAALEGKNQEGKK"
     gene            complement(8700..9419)
                     /locus_tag="VEW08_05850"
     CDS             complement(8700..9419)
                     /locus_tag="VEW08_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40293.1"
                     /translation="MISDEELLLYYYRELDSGERARIGAAVSNDPQLSRRLHNLVARL
                     DAAAAMPEVPVPAHTQRRWQAALARATSDKRVAPPTRGFFTQLRWQAAAAAVAVVAIT
                     LTIQNVKSPSSIKTAGETVEASPPGATSAGDASAYEHGLKWHLASTEKRLASLGDATP
                     EERARLVETIIGQNRIYALAAERAGEPQLARVLRAFTPVLESVADGRVESASSVAQLN
                     FELRVMQARLAADAAAPANTL"
     gene            complement(9416..9949)
                     /locus_tag="VEW08_05855"
     CDS             complement(9416..9949)
                     /locus_tag="VEW08_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYJ40294.1"
                     /translation="MLTLLKSWVRPDAFHAVLSERSGRWYNACLRITRDADLAADAVQ
                     DALLKAWDRRDDFRGEAALDSWIHRIALNAAIDLTRRRKTRAENELDDDSFEAAPSAS
                     PESEYVVEALSKDLATAMQRLSAMERQVFLLKHIEGWRLDEIAESLQTTINGVKHALF
                     RAVSKLRVDLHVWRGES"
     gene            complement(10045..11337)
                     /locus_tag="VEW08_05860"
     CDS             complement(10045..11337)
                     /locus_tag="VEW08_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HYJ40295.1"
                     /translation="MRRAHNSLVVLFGFFFLLVGVTLSGCGGSEGSPPDDAPPVNEIS
                     SNKARVTAPVVSSGDAATFANDNLAFSVDMYLALRSSQPGNFLFSQTSISTALAMLYA
                     GAGTTTAAQMSDTLHFSLPAARLHAAFNALDLALTTPPSTSNGFQLTFANSLWIQKDF
                     SVLPGFLDTLAENYGAGLFVEDFQSAPEPARNTINGWVAHRTEGQIPSLFPQGSINTL
                     TRLVLANAVYFHGDWKVKFKKDSPNEIFHTLSGDVSVPTMHGANNAAIWSGAGWNAGV
                     LDYVGNTTSMIIVVPDAGTFAAFESGLTFESLSAILAGAGSGGRADLIMPRFKFSTDV
                     GLNSTLSALGMPEAFSDGADFSGINGGRDLRIQSVIHQAIINVDESGTTASAATGVSV
                     GVTSLPPTLVVNRPFLFFVRHNPTGAILFQGRVVDPSK"
BASE COUNT         2081 a         3631 c         3667 g         2058 t
ORIGIN      
        1 gacggcgtca cgaaacacca gcgcctcatt gaggtcacgg ctgaaacatg cgtcgctgtc
       61 gatatcgagg ctggcgaaac acgccagtac aacgcccgtg ggcccgcctt cgagcgtgtt
      121 cacgaaatcc cgcaccttga tcttgtagta gtccaccgag aggtgcaggt cgggaatggc
      181 ttccggcgta agggcaaaac cgaaagtcag cgtgtccgaa gtttcctcgg agagattcgt
      241 gttgccatag aagagcgcct cgatctggct gttggtctgc gtgtaggtcg ccgcattgat
      301 tccccaggtc tgctggcaga atgccgcgac atccgccgga acgacacgag tcgtgccgtt
      361 ggcgcgcgtc gatgcgcacg gatcgttgac aatcggaaat cccttgtctc cggcctggaa
      421 cagttcgaac acgctgggcg cccgcacggc gcgctggtac atgccgcgca cgtgcaacga
      481 cgacatcggc gcccagtcga ggccggcctt gtatgaagtg acttcgccca ccgacgaata
      541 gtcggaatag cgcagaccgg cttcgaggct cagcgacttc accgcgggaa gatccttcag
      601 cagcggcaca acggtctcgg cgaacaattc ggtgacgtcg aattcccccg cgacgggttg
      661 cgccgcattg aagccgagaa tgtctccctg cttcttcgcg aggtccggta cgtactccga
      721 actgtccttg cgatattccg cgccaagtgc gaagcccagc gggccggctg gcaattcggc
      781 gagggtgcca ttgatggacg cgttcgccag ctgccgctcg aagatggtcg aatcggaaaa
      841 atcgagacgg atgaagttcg ccatctcagg cgtaatcgat ccgacgccga atgcgttgac
      901 gggcacgcag cccggtacga atttcacgta ctcagccgga cagttggaca atccggccgc
      961 gaacttcgaa cgcgagacgt cattgtgcgt gacgttgatg aagtcggttc gtccgaactg
     1021 gtagctcacg tcgaactgcc agtccttgta cggcaggtcg ccgcggaaac ccacgtccgc
     1081 ctgctgcagc ttggatttgt tttcctgcag acgggggccg atttcggtca tgcgacgcgt
     1141 gaggaacccc ggtgcattcg gattcggccg tgcatccatg agcgcgcgcg cctgcgcgga
     1201 cagcagcggg ctgtccggat cgaacggcac gttcaccgcc ggcgtgggcg cgagctgcgc
     1261 tgcgttgcgg ctgtcgacgt acatgatgtc catgtaggcc gtgagcttgt ccgtgatctt
     1321 cagattgccc atggcgccga tgttgatgcg ctcgccaggc gtgagcagga agttgacggg
     1381 cgagaagttg tacttgtcgc cctgtccatc cgcgctggtt tccggcagcg tgttattcca
     1441 cacgcgcacg ccaccgttgt tgttcaggtt gaaggcgtag tcacgcgaag tgctgccacc
     1501 gccgaggaag tctcccacag tcgggtaagg attcaagccg acattgggca gccgcgcgtt
     1561 gccggtgggc gagccgttcc cgctgcggtc cacgcgcgtg aactcgcgtt ccgactgcag
     1621 caccgagtcg cggtcgaaat agctggcgaa gacggtgatg tttccacggt cttcacccag
     1681 gtttccacct aacaacacgt cgaactggct ctgggcgccg tcgccctgcg cggactggcc
     1741 gtgctgaatg ccgaagtcga agccctggta gtcctccttc atgatgaagt tgaccacgcc
     1801 ggcgagcgcg tccgagccgt acaccgccga ggcgccgccc gtgaccactt cgacgcgatc
     1861 gatcaggcgc accggcacat tgttcaggtc gaccacgccg gtctgcgtcg atggcgtcag
     1921 ccgccggccg ttgatgagca ccagcgtgcg cggcgtgccg atgcctcgca gatccaccgt
     1981 cgacgttcca ttcgccggat tgttcgaatt ggcggtgagt cccggcacca cctggggcaa
     2041 ccggttgagc acctcctcga cgctgagctg gccggtggcc tggatcgcgt cggccgacac
     2101 ggtcgtcacc ggactgatgg cctcgtagtc gcgaacgcga attcgggagc cggtcaccac
     2161 gacttcttcg acaacctcgg cggcgggcga agacggatcc gcggcaagag ccgggagcac
     2221 aggcacggcc gacgcaaacg ccccggccca caatgcgcgc cgcacggcgc gtcgaacgga
     2281 ataacgaaac gacatcagat ttctcctcct gcgagagcag ctggtgtgtg accgtaacaa
     2341 cagcgcctgg cgccattcgt gcagcaattg cgaattcagc gggctgcgat tgcgaatctc
     2401 acgccgaggt tcgcgaggcc gcggcgtcgc acatgcgtat ccgcaactgg ctcggcgaca
     2461 acttcgtccg gcgtcgcacg tgccgcagca gtgtcgactc gaatgcaaag ccgactctgg
     2521 cggtcacttc gctgatcggc agccgcgttg attccagcag gcgcaccgcc acgtccacgc
     2581 gcttgcgctg caggaatttg cgcggcgtga tcccgtggat cagcgtgaac agcctgagga
     2641 agtgatattt cgatagatag gccatgcggg ccagcgtgcc gagatcgaca tcttgcgcat
     2701 agttcgcgtg aagatagtcg gtggccatcg caatgcgccg gaaagcttcg cggcgcgtcg
     2761 ccgggcgaat caggtcgatc tggtcgatct gctcgtgcaa cttcgcctgc cgggtgcgca
     2821 tgcgctcgag caggaacacc agctgttcct cataccagtc ctcttcgaca aaaccacgcg
     2881 ccagatgcgc ccggatgaaa cgcagcactg gactcacggc gctgtcgtgc ggctgcaggt
     2941 tctccaggaa ttccggctct tgtgtcgcaa ggccgccggg ctgcggggca aaacagatgg
     3001 tgagcgactc cactggatcg gccgcgtgaa tactcgttgc gcagttccgg ccggggttca
     3061 ggatgaggaa attgtcatcg tcgaccgcaa gggttcggcc atccacgtgg cagaactgcg
     3121 cgccgcccca caccgcccgg atagagagca attgcggata cggcccgaac tccgcgctcc
     3181 tcgaacgtcc ccagatgatg gaactctccc gtgaccacct ctcacggaag gattcgctca
     3241 gcgcggactc cggcgcggaa agctcgggaa gcgaacgcag tagcaacatc tcaggtcatc
     3301 cgaagcgggt cagcgcacga cgccgacggt ggccagttga gtggcgcggt tggcgggctg
     3361 cggctgttgc gagatgccgg taagcgcgcc cgacagcgga tcgatgccga aactcgcaac
     3421 gctgttgtcg aacgacagca cccagatggc attgccgctg aaatccgcca gcacctggcg
     3481 tggctccgtc accagcggcg ttcgcccgat ctcgacgggg gcgccggtgc gctgatccag
     3541 cgagaacgcc gaaatcgtgc tcgaattcga gcccgcggtg tacaggaatc gcccgctcgg
     3601 atcgatcgtg agcgacaggc tgccggggtc gatgcccagc acgctgccat cgcccagcgc
     3661 gccgctgcgc gaatcgaccg gaaaccgctc cagcatgccc tgcgggctaa ctacataaag
     3721 ccatcggccc gacggatcga aggccgcctg cgtgccgagc gcgctcgcaa gttctttcaa
     3781 gccgccctgt tcgtccggga gcgcgtgcgc ctcacccgtg accgaatcga gtccgaagac
     3841 ccggactcgc gcgaaactca tcacgtagag attgcggccg gtcggatcga tgaccatcga
     3901 cagtggcccg ggtccggtcg cgaagggagg tccgatcgcg gtgagtgatc caccgttccg
     3961 ggcgcggcgg tatgccgtca ccgtgccttc atcgaaattg ccgacataca cgaaacgatt
     4021 gccgggatcc gcgacgatcg cggccgggct cgcgccggcg ggcgtggtac ctgcctcgat
     4081 cgccgtcagt gcgccgttcg cgttgtcgat ggcgagcgtc gtgacggatc ctgcgagtcc
     4141 ggccacgtga agaaacttgc catccgtgct ggcaacgagc gcgcgcggca gcgtgtcggg
     4201 aagcgagatc gagccgctga cccgcagcgg cgccgtggtg ggatcgatga cgaacgaagt
     4261 gaccgagttt ccgggcgtgt cggccatgta cagaaacctg ggcaggaata ccgcgggcgt
     4321 cgcgacgact ggcgagacga atgcatcgat cgaggttggc tggcttcccg cgcgaatcac
     4381 cgcgccgcgc gaaatcggct gcaattcacc ggcggcgccg atgctgtacg catgaatgcg
     4441 atcgcccgag agaccggctg catacagata tcgcccgccg ggatccacac tcgcggtgaa
     4501 tggcgctggc tgcagcgcat cgatgccagg aatcctggcc agcacaccgg tgtcgcgatc
     4561 gaccctgaac gcggtgacgg atcctgttcc agctggctca gctggacctc gtgaacgatt
     4621 ggcgacgtaa agaaattcat tggacggtgt cagcgagagg ctgcccgggc tgaagccggc
     4681 ctcatacggg ctgcccggca gtggagtgag aacaccggtc gtcagattga ccgagaagcc
     4741 ggcgacggca ccgcgtacgg ccggcgtacc gaagctcatc acatacgcaa accggccgct
     4801 gacatcgaaa acaggaacac cgcttgcctg caccggcaga tcgtgtggct gtccggcgag
     4861 cggcgtgagc tcgccggtcg tcgcgtttac ggcaaatgcc gcgaggcttc ccgggttgcc
     4921 cgcaaagtcc tggttcacca cgtaggcgaa tttgccgccc ggatgaaaca cgaaattcga
     4981 ggtgcgcaga tcgttgttgg tgaactgcac gcggagtccg gcgcgttccg caagcgcacc
     5041 ggttgccgcg tccgcttcgt agacgtagat gccgtcgctg ccatgatcca cgtgcgtcag
     5101 ataggcaaat cgcccgcgag cgtcgaatac gagttcgcgc ggcgcatttc cggcgggaac
     5161 gaggtcgtcg ttgatcggcg tcagcgcacc gtccggcgcg acggaccatt gaaagatcgc
     5221 gtcgtcacga gtcgtgacgt acacgaattt tccgctcggg tgcaccgtca aagcgctggc
     5281 cccgacgagc gaatgcgagg tgacgaacgg actgccgtcg atctcctgca gatcgccatt
     5341 cacgttgtca tgagcgaaca ccgaaatgga cgagaaggac agcggagccg cgggatcgcg
     5401 gtcgatgccc gagttgagca cgaagctgaa ttttcccgcc gggtcctgca cgggccgctg
     5461 tcccccggcg ccggtcttga cgtatccgcg cgcacgaagc tggccggtgg ccgcatcgac
     5521 attgtagata gagatggagt ccgatccgaa gttgaatgca taggcgaagc gcggcgccgc
     5581 ggtggttaca cagcgcacgg cgacggctgc gtcggcgttc gcaaccgcgc cggtgtccgg
     5641 cgataccgtg catgcctgcg ccggcgcggc tggttgcgaa gccaccgtca ccgcaaatgg
     5701 ggctccgtcg ggcagcgcgg tgggaaacct ggattgtccg ttggccgtga tcgaaaccgt
     5761 gtcgacgccg ttcctgagcg caagtcccga gccaacgaga cccgttaccg tgaagccaac
     5821 aaagtagcta gcgggtggtg gcgcccctgc gcctccggat ccgccgcagc tcacgagcaa
     5881 cagcgatgcg accagcatcg acagggccct tgctcccgcg ccgggcctgc ggcgattcca
     5941 ttgaagactc atctgatacc ccaggttcgt cggccgcgtt tgcgcccgct ctatgggtaa
     6001 tagtcgccat cgactcccca tttacgacag acgcaaaaaa gcttccaaac cggcgaaccg
     6061 cgcgacaatt cggcggtaca taccgtggga gtcttcgtga acaatggggt accgcagtgt
     6121 cggagatcgc gtttctcaga gccctggaag ccgggaggct cgacgatcac cagacattgg
     6181 gcgatctgtt cacctcgttc tatggagagc tccaccggcg ggcaaaatgg gagttgcggc
     6241 agaacacacc gctttcactg gggccaacca cgctgctgca cgagaccttt ctggacatgt
     6301 cgaatcgtga atcggtgtcg ttcaccgatc accgcaaatt catcgcctac gccgcgcgcg
     6361 cgatgcgggg gctcatcgtc aaccacttcc ggaatcgcca tgcccagaag cgcggcgggc
     6421 ggctggaatt cacggcgctg cacacggatc tgcctgagga caggcagtcg atcgacatcg
     6481 agggtctggg cgaagccctc gaatcgctgg caaccctcga tccccgcctc gccgaatgcg
     6541 tcgacatgaa attcttctgc ggcctttcgt tcagcgagat cgcgaatctg cggaacgtgt
     6601 ccgagcgcac agttcagcgc gactgggaca aggcgcgact tctgctcaac cagctgatga
     6661 tggttcacga tgaccggccg aaggccgggt gaagagcatc gacagcctca gcgcgtttca
     6721 gttggctgtg ttccgcgcga gcaccttttc caggtacgcc tgcgctgaat ccgactccga
     6781 ctgtgacaac cagaacacgg cccgcttgcg ttgctcgcgg gagagggact gatcttccgc
     6841 cgtggcgatg agcgcgcgcg gcgcccggtc gtccggcaac tgcgacagcg cgaacactgc
     6901 gtgctcgcga acatgttcgt cggcatcctt ccgaagcgct gcggcaatcg cctgttccgc
     6961 ctcggccgct cccgtctgcg cgagccagaa ccacgcctgg gcgcgaacat cgccgacctt
     7021 gtcggtgttg ccctgccgga tcaggtccgc ggcggcccgc ggtgatttgg tttgggccag
     7081 cgcgaaggcc gcgtgtttgc gcacgtcctc atccttgtcg ttgaacatca cggtgctcgt
     7141 gatggccgcg ccttcgtcgc cgcgcagctg ggcgagccag aaaaccgccc acttgcgggt
     7201 ctcgatacgt ggatcacgcg agaactctgc caatgcatcg cgcgcaaagt caccgcggtg
     7261 cattgccagc gcggccagcg cactctcgct gatcgggtta cccgtcgccg catcattgcc
     7321 gctttgcctc gcgcgggcga tgagccaacg ggcactgtct tctaccgtga cattgccgag
     7381 ctcgttgacc ggcgtcctgg tttccacctg gcacgtcgcc gacagcacct gcaggcgatc
     7441 gatcctgccg ccggctacgc gcgcgtacac cttgatcgag tcagtcttct cgtcgctccg
     7501 tgtaccgtaa tggtcgggcc tgtcatcgag tcgacatgac gtcggcgaga catcgcgatc
     7561 cttccagcta ctccagcagc accaggccgg agcattctca gcggcggaaa cctgccagct
     7621 cgcccaaccc tcgcgaggca gcgtcaactc agccgcatgc aactgcggga cggccgcgtg
     7681 agccagcaac agtgcaaacg cgcacaccgg aagattgagg gagttcattt cttcccctcc
     7741 tggttctttc cttccagggc ggcatcgatc gcctgcaacg cggcgtcccc atccatcatc
     7801 gacaggtagc gcaacagcat gcgtttctca ttgctcgcct tggcgccccg gtagagcgtc
     7861 aacaccccct gctcgtcgcc ggacatcaac atgccctgca gcgcagcttc cttgatctcc
     7921 tcatcggtgc tgcgtgaata gatatcgcgc aacgcggcgc gtgccccgtc gccctgaacc
     7981 atgccgatct tgcgaatcgc atcgaggcgg agggaacgat ctccacttcc ttcggcgatc
     8041 ttgcgcaggc tggcgaggtc gccgctgatt gcaaacgcat ccaccaagcc actggcggcg
     8101 ttcggccggt caccgagctt gcggagttcg tccgtagcac ccatcaccgc gagcatgcga
     8161 atcgcttcgt tcgactcctc ttccgatttg ccattcagcg cggcctggta aacagcctcc
     8221 ttccgcccgg cgatcatcca ggcctgcaac acgtctcttt tcacgtcggg atcgccggcg
     8281 ttgtagacct cctgcagggc atcgagcgcc ttcggatcgc caccgatgcc aatgctgcga
     8341 atcgcttcac ctcgcagggc ggcgtccgat gagcggctcg tctgggccag gatcgcctgc
     8401 gcttccggcg tgccgatctg gctgagcacg aacagcgcgc gctgcttgac cagcgtggtc
     8461 tgcgatcccg ccagcacttt ttttatgatg ggcagcgcgc gctcggcggg ttgcgccatc
     8521 aggccttcga gcgctgccag cgcgagatct tcttcggccg agggcgcacg ctctgttttg
     8581 cccgggcgtt cgcgttgcgc ggccggcgcg gccgccggtg gcgtctgtgc gttcgccgtg
     8641 ccgcagatcg ccagcgcgac cagaatagtt agtaagtaac gcatgattgt tcctcgaagt
     8701 taaagggtat tggctggcgc tgcggcgtcg gcggccagcc gggcctgcat gactctcaat
     8761 tcgaaattca gttgcgcgac gctgctcgcc gactcgacgc ggccgtccgc gacactttcg
     8821 agaacgggcg tgaacgcgcg cagcacgcgc gcgagctgag gttcaccggc gcgctcggcc
     8881 gccagtgcgt aaatgcggtt ctgcccgatg atcgtctcga ccagccgggc ccgctcctcg
     8941 ggagttgcgt cgcccagcga cgcgagccgc ttctcggtgc tcgcgagatg ccatttcaat
     9001 ccgtgttcat aggcggacgc atcgcccgcg ctggtggcgc ccggcggtga agcttcgacc
     9061 gtctccccgg cagtcttgat cgacgatggc gacttcacgt tctggatggt cagggtgatc
     9121 gcgacgaccg ccacggcggc cgcggccgcc tgccatcgca gctgcgtgaa gaacccacgg
     9181 gttggcggcg ccacgcgttt gtcacttgtc gcgcgtgcaa gcgcagcctg ccaacgccgc
     9241 tgcgtgtgtg cgggcaccgg aacttcgggc atcgccgcgg ccgcatcgag cctcgccacg
     9301 agattgtgca atcggcggga gagctgcggg tcattggaga ctgccgcgcc gatgcgcgcg
     9361 cgttcgcccg agtcgagttc gcggtagtaa tagagaagga gttcctcatc ggaaatcatg
     9421 attcgcctct ccagacatgc agatcgacgc gcagcttgct gaccgcgcgg aatagcgcgt
     9481 gcttcacgcc gttgatggtg gtttgcaggc tttcggcgat ctcatcgaga cgccagcctt
     9541 cgatgtgctt gagcaggaag acctggcgct ccatcgccga caggcgctgc attgccgtcg
     9601 ccaggtcctt gctcaacgcc tccacgacat attcgctttc gggagaagcc gacggagcgg
     9661 cctcgaagct gtcgtcgtcg agctcgtttt ctgcccgcgt cttgcgccgc cgcgtcagat
     9721 cgatggccgc gttgagcgcg atgcgatgaa tccaggaatc gagggctgcc tcgccgcgga
     9781 aatcgtcgcg acggtcccag gctttcagca gcgcatcctg gaccgcgtcc gcggccaggt
     9841 ccgcatcgcg cgtgattcgc aggcaggcgt tgtaccagcg tccgctgcgc tcgctgagca
     9901 cggcatggaa ggcgtcaggc cgtacccatg atttgagaag tgtgagcacg gaacttggac
     9961 ggcggcggcg ccaaaaggtt aggtaccgaa gattctggcg ctgttcaggg ccctcccggc
    10021 gtcgggttcg ggcagcgcct ggccctactt cgacggatcg acgactcggc cctggaacag
    10081 tatcgcgccg gtcgggttgt ggcggacgaa aaacaggaac gggcggttga cgaccagcgt
    10141 gggcggcagc gacgttacac caaccgaaac gccggtggcg gcagaggccg tcgttcccga
    10201 ctcatcgacg ttgatgatcg cctggtggat gacggactgg atgcgcaggt cgcgcccgcc
    10261 gttgatacca gagaagtcgg ctccgtcact gaacgcttcg ggcatgccca gcgccgacag
    10321 tgtgctgttc aagccgacgt cggtggagaa cttgaaccgc ggcatgatca ggtcggctct
    10381 gccgcccgaa ccggcgcccg ccaggatggc gctcagcgat tcgaaggtca gcccggattc
    10441 gaaggcggca aacgtccccg cgtccggaac cacgatgatc atcgaggtcg tgttgccgac
    10501 atagtcgagg acgcccgcat tccagccggc cccgctccag atcgcggcgt tgttggcgcc
    10561 gtgcatggtc ggaacagaga catcgccgga gagcgtatgg aagatctcgt tcgggctgtc
    10621 cttcttgaat ttcaccttcc agtcaccgtg gaagtagacg gcattggcca gcacaagccg
    10681 tgtgagcgtg ttgatcgaac cctgggggaa cagcgaagga atctgaccct ccgtgcgatg
    10741 cgcgacccag ccgttgatcg tgttgcgtgc gggctccggc gccgactgga agtcctcaac
    10801 gaatagtccg gcaccgtagt tctcggcaag cgtgtcgaga aagccgggca gaacggaaaa
    10861 gtctttctga atccagagtg agttggcgaa cgtgagctgg aaaccgttgc tcgtggacgg
    10921 cggggtggtc agggccaggt ccaatgcgtt gaacgcggcg tgcagccgtg cggcgggcag
    10981 gctgaagtga agtgtgtcgg acatctgcgc cgcagtggtc gtcccggcgc ccgcgtacaa
    11041 catcgcgagc gcggtcgaga tgctcgtctg cgagaaaagg aaattgcccg gctgactcga
    11101 ccgcagcgcg agatacatgt cgacagagaa tgcgaggttg tcgttcgcga aggtggcggc
    11161 atccccgctg ctgaccaccg gggcggtcac gcgcgccttg ttggagctga tctcgttcac
    11221 gggcggggcg tcgtccggcg gggaaccctc agagccgccg cagcccgaca gcgtcactcc
    11281 gaccaacaga aaaaagaaac caaacaacac cactaacgag ttgtgcgcac gacgcatggc
    11341 cagtccctca caaaatgacg ggcgagcgtt gcacgggacg cgtcttgtgt ctgtcaagaa
    11401 ctgtggtggt ggaacgcagc caactacttg tttggga
//
LOCUS       DASQJS010000142         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1644747, whole genome shotgun sequence.
ACCESSION   DASQJS010000142 DASQJS010000000
VERSION     DASQJS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644747"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..338)
                     /locus_tag="VEW08_05865"
     CDS             complement(9..338)
                     /locus_tag="VEW08_05865"
                     /inference="COORDINATES: protein motif:HMM:NF023232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3325 domain-containing protein"
                     /protein_id="HYJ40296.1"
                     /translation="MYFLSALLCAYAGFAALCAITARNGALVWRKPPSQGVTRALRAA
                     GFLLLALAWVPCYLNWDVPMGTAAWVMILAASGFALVFPFAYAPKRSVQAGGIAAPLA
                     LIALLLG"
     gene            complement(338..2047)
                     /locus_tag="VEW08_05870"
     CDS             complement(338..2047)
                     /locus_tag="VEW08_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HYJ40297.1"
                     /translation="MEKSFTQRMDWLHTWFGLVLGWLAFALFLTGTIAVFWFEIQHWA
                     QTELHGAKMLSLEDTVQYSVDYLEKVAPDSRRWAVTLPDLDRHPLLVLNWLDENDAQQ
                     FVRLVPDGSGTEVTTVTQGGRFFVDFHWTFNRPVHDLTGGTFQWTYMLSGAVGLAFLI
                     CTITGLVVHKKIFRNFFTFRRQAKSVQTRWLDAHNVLGVISWPFQFVIVLTGLAFYCY
                     LYIPSGMQMASRYSEPPRAAVVGGGGPSMLWGRFTADAVGRVPGNIVAPGNPAPNVPV
                     VELFARAQAEMGTVSSFTVNNPHRDNATVRFGATRAPTHEITFTSDNMVFDGATGAVI
                     SPAGNTINAVQKVTNVLGGIHFAFFGGTAMRVLYFLCGLAGTIMIGAGLVMFMAKRRA
                     KARSAAAARFYGFVDRMNVAAIGGALFACASYFWALRLLPQSLSDPSGGFPGVGVYAA
                     IRAVPLDQAARSDLELYAFYFAWGVAGAHALLRAPHKAWFEQFAAVAALCIGLPVIGY
                     LVPNSDIVSMIAAGDWKVVSVDLAAFGIGLALASAAWKMRATRADVREGRAVTTPVLS
                     PAD"
     gene            complement(2088..2372)
                     /locus_tag="VEW08_05875"
     CDS             complement(2088..2372)
                     /locus_tag="VEW08_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40298.1"
                     /translation="MSYTRLVVSRVAAAVLGGYAVTYWAGAAAAKLTIEWNIFNSVDA
                     GMFSGLVQLVVYTTAIISVCAAASMKRAWWALAACTALLIGIVVLMPGPP"
BASE COUNT          527 a          893 c          862 g          410 t
ORIGIN      
        1 ctgaaccctc agccgagcag caacgcgata agcgccagcg gcgccgcgat accgccggcc
       61 tgcacgctgc gcttcggcgc gtaggcgaac ggaaacacga gagcgaaccc gctcgcggcc
      121 agaatcatca cccaggcggc cgtgcccatc ggcacgtccc agttcaggta gcacggaacc
      181 cacgcaagcg cgagcagcag aaaacccgcc gcgcgcagcg ctcgcgttac gccctgcgat
      241 ggcggcttcc gccaaacgag cgccccgttg cgcgcggtga tcgcgcagag cgcggcaaag
      301 ccggcatacg cgcaaagaag ggcggacaga aagtacatca gtccgccggc gacagaacgg
      361 gagtggtcac cgctcttccc tcgcgcacgt cggctcgcgt ggcgcgcatc ttccacgccg
      421 cactcgccag cgcgaggccg atcccgaacg cggcaagatc gacagacacg actttccaat
      481 cgcccgcggc aatcatcgag acgatgtcgc tgttcggcac gagatagccg atgacgggca
      541 gcccgatgca cagcgcagcg accgccgcga actgctcgaa ccacgctttg tgcggcgcgc
      601 gtagcaacgc atgcgcgccg gcaacgcccc acgcgaagta gaacgcgtac agctcgaggt
      661 ccgagcgcgc ggcctgatcg agcggcacgg cgcggatcgc cgcgtacacg ccgacgccgg
      721 gaaagccccc tgaaggatcc gagagcgact gcggcaacag ccgcagcgcc caaaagtagc
      781 tcgcacaggc gaacagggcg ccgccgatgg cggcgacgtt catgcgatcg acgaagccgt
      841 agaaacgcgc tgccgcggcg gaccgcgcct tcgcgcggcg cttcgccatg aacatcacga
      901 ggccggcgcc gatcatgatc gtgcccgcca ggccgcagag gaagtagagc acccgcatcg
      961 cggtgccgcc gaagaacgcg aaatggatgc cgccgagaac gttcgtgacc ttctgcacgg
     1021 cgttgatcgt gttgcccgcg gggctgatca cggcgccggt agcgccatcg aacaccatgt
     1081 tgtcggacgt gaaggtgatt tcgtgcgtgg gcgcgcgcgt ggcgccgaat cgcaccgtgg
     1141 cgttgtcgcg gtggggattg ttcaccgtga agctcgagac ggtacccatc tcggcctgcg
     1201 cccgtgcgaa gagctccacg acgggcacgt tcggcgccgg gttgccgggc gcgacgatgt
     1261 tgcccggtac gcggccgacc gcgtcggccg tgaaacggcc ccacaacatc gagggcccgc
     1321 caccaccgac cacggccgcg cgcggcggct ccgagtagcg cgaggccatc tgcatgccgc
     1381 tcggaatgta gagatagcag tagaacgcca agccggtcag cacgatcacg aattggaacg
     1441 gccaggagat gacgccgagc acgttgtgcg cgtcgagcca gcgggtctgc acggattttg
     1501 cttggcgccg gaacgtgaag aagttgcgga agatcttctt gtgaacgacg agccccgtga
     1561 tcgtgcagat caggaacgcg aggcccacgg cgcccgacag catgtacgtc cactggaacg
     1621 tgccccccgt caaatcgtgc acgggacggt taaacgtcca atggaagtcg acgaagaagc
     1681 ggccgccctg cgtgacggtg gtgacctcgg ttcccgagcc gtcgggcacg aggcgcacga
     1741 attgctgcgc gtcgttctcg tcgagccagt tcagcaccag cagcggatgg cgatcgaggt
     1801 ccggcagcgt gacggcccag cgccgcgagt cgggcgcaac cttctcgagg tagtccacgc
     1861 tgtattgcac cgtgtcctcg agcgacagca tcttggcgcc gtgcagctcc gtctgcgccc
     1921 aatgctgaat ctcgaaccag aagacggcaa tcgtgcccgt aagaaagagc gcgaacgcca
     1981 gccaaccgag aaccaggccg aaccacgtgt gcagccaatc catgcgctgc gtgaagctct
     2041 tctccatgat tctgtctccc cccggagcgc tgccgttctt atggagccta aggtggtccc
     2101 ggcatcagca cgacgatacc gatcagcaga gccgtacacg cagccagtgc ccaccacgct
     2161 cttttcatag aggccgctgc gcacacagag atgatggccg tggtgtagac cacgagctgc
     2221 acgagaccgg agaacattcc ggcgtccacg ctattgaaga tgttccactc gatggtgagc
     2281 ttggccgcgg ccgcgccggc ccagtacgtg acggcatagc cgccgagaac tgcggcggcc
     2341 accctgctca cgacgagccg ggtgtacgac atatcggaag tcttggtatt ccgaacgctg
     2401 acggccaagg catcccccgt gttttgccgt attatgcgcg gtttgcttgg ctgttcaagc
     2461 gcgcccgctt cctgacactg gctcagtttg aatatctaag cgatgagcgc cagtgatgct
     2521 atcgctcggc caccgcgctc ctcagcgcgt cctgcagggc agacggcggc atacctacct
     2581 gctgagcggt gttcaattgg caggtcatca acttcgctgt gatggcatac atccgatcct
     2641 ccagcgcgtc cgccggggtt tcagtcagta ccccgggtgt gttcatcgct tg
//
LOCUS       DASQJS010000143         3832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_703256, whole genome shotgun sequence.
ACCESSION   DASQJS010000143 DASQJS010000000
VERSION     DASQJS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703256"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="VEW08_05880"
     CDS             complement(<1..1589)
                     /locus_tag="VEW08_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HYJ40299.1"
                     /translation="MSSSPNCWHCGEALQKGVVIHARVAGASHPMCCQGCRAAAEWIE
                     QLGLSDYYALRTAAAQKPARDADPETHDYWQTDNARHVIRDLGDGLRESLLLVEGVRC
                     TACVWLIERSLGAMPGVMSVQVNAVAQRARVIWRDSKISLPQLLQGLARTGYHALPLD
                     ARGLDDLRRRESRGALKRLLVAGFGAMQAMMFATAIYLGAAASLDASTRDLLRWLGLM
                     VATPVVFYSPRPFFAGAWRSIKTKRIGMDVPVALAIAAVYAASVIEALRGSGEVYFDS
                     ISMFVFFLLAGRYLEMRARHRSCDLTDALARLWPPFADRKRDDGTLQRVGIHELRVGD
                     CVRVGEGGIVPADGVLLTERCRVDEALLSGESAPVTKRCGELLIAGSVLEDGPVLVRI
                     ERTGADTTLAGIAALVGRASAARPQLQRAGELAAGRFVTRVLALTALTAAVWAVVDPA
                     RAFSAAIAVLVISCPCAFALAVPAALTRALGALARRGVLVVKPDAIQTLAECTHAMFD
                     KTGTLTEVTLSADVQTFGGVSR"
     gene            complement(1712..2632)
                     /locus_tag="VEW08_05885"
     CDS             complement(1712..2632)
                     /locus_tag="VEW08_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYJ40300.1"
                     /translation="MSGFWSAWVIVLACVTVGISVALFVWALWMRIPTASDGTTGHVW
                     AHGALREAVRPLPWWWVLISIGAFMFGVAYLSLYPGFGSFAGRLAWTSKGELQRHTAA
                     NDVLLEVRLAPLRGLSFEELAVHKEAVSFGHRLYLDNCAACHGREAQGNQAVGAPDLT
                     DADWLYGGDAETILTSILDGRNGVMPPLAGALGHNGTNEVAAYVVSVSGTQVPPDWVA
                     AGKVRFDALCVACHGADGRGNPAMGAPNLFDSAWLYGGNIENVVASVRDGRNGVMPPW
                     RQRLSADEARLITAWILAQGRRVAPQVAAK"
     gene            complement(2629..2778)
                     /locus_tag="VEW08_05890"
     CDS             complement(2629..2778)
                     /locus_tag="VEW08_05890"
                     /inference="COORDINATES: protein motif:HMM:NF017366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HYJ40301.1"
                     /translation="MSELYGHFIGVMIVVIMLIFIGIWIWAWLPHHEKDFSELARLPL
                     DEDSP"
     gene            complement(2775..3410)
                     /gene="ccoO"
                     /locus_tag="VEW08_05895"
     CDS             complement(2775..3410)
                     /gene="ccoO"
                     /locus_tag="VEW08_05895"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HYJ40302.1"
                     /translation="MGSYKHWEKLEKNALLLGVLIAVVVSFGGLAEIIPLFNDAQTVR
                     PAPGVTPYEPLRLAGRDVYVREGCYLCHSQMIRTLSFETQRYGARSEAGEFVYDRPFQ
                     WGSKRTGPDLARVGGKYSDAWQRAHLLAPRQLVPRSNMPGFPWLAETKVDGEYLQAKM
                     RTLRTLGDPYTDAQIAGAAEAVLDKTELDALIAYLQGLGVKAVPATAAVTP"
     gene            complement(3413..3757)
                     /locus_tag="VEW08_05900"
     CDS             complement(3413..3757)
                     /locus_tag="VEW08_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HYJ40303.1"
                     /translation="MNLHFWLHTTGLLLYIVAMWAAGVTQGLMWRETNADGSLAHDFL
                     SSIIATQPYYLARFLGGVLVLSGMVVMAWNLWHTAANARAGVIKPILVPAPETIPEPV
                     PDQFPAPLPERV"
BASE COUNT          707 a         1301 c         1244 g          580 t
ORIGIN      
        1 cgcgacacac cgccgaacgt ttgcacgtcc gctgacagcg ttacttcggt gagcgtgccg
       61 gttttgtcga acatcgcatg ggtgcactcc gccagcgtct ggatggcatc cggcttcacg
      121 accagcaccc cacggcgcgc cagtgcgccg agcgcgcgcg tgagcgctgc gggaacggcg
      181 agcgcgaacg cgcaggggca ggaaatcacc agcacggcga tggccgcgga gaatgcgcgc
      241 gccggatcga ccacggccca cacggcggcg gtgagcgcgg tcagcgccag gacgcgagtg
      301 acgaatcggc ccgcggccag ttcgcccgcc ctctgcaatt gcggacgcgc agccgacgcg
      361 cggccgacga gcgcggcgat acccgcgagc gtcgtatccg cgccggtgcg ctcgatgcgc
      421 actaacacgg gaccatcctc cagcacgctg cccgcgatca gcagctcgcc gcagcgcttc
      481 gtcaccggcg cggactcgcc gctgagcagc gcttcgtcca cgcggcagcg ctcggtcagc
      541 agcacgccgt cggccggcac gatgccccct tcgcccacgc gcacgcagtc cccgacgcgc
      601 agctcgtgaa tgccgactcg ttgcaacgtc ccgtcgtcac gcttccggtc ggcgaacggc
      661 ggccacagcc gggccagtgc gtcggtgaga tcgcacgagc gatgccgcgc ccgcatttcc
      721 aggtagcggc ccgccagcag gaagaacacg aacatcgaaa tcgaatcgaa gtacacctcg
      781 ccgctgccgc gcagggcttc gatgacgctg gccgcgtaca cggcagcaat ggccagcgcc
      841 accggcacgt ccattccgat gcgctttgtc ttgatcgatc gccaggcgcc cgcgaagaac
      901 ggccgcggcg aatagaagac caccggcgtc gccaccatca atccgagcca gcgcagcaga
      961 tcgcgcgtgg aagcatcgag cgacgccgcg gcgccgaggt agatcgcagt ggcgaacatc
     1021 atggcctgca tggcgccaaa gcccgcgacc agcaaccgct tgagggcacc acgcgattca
     1081 cggcgccgca ggtcatcgag accacgcgcg tcgagtggca gagcgtgata cccggtgcgg
     1141 gcgagaccct gcagcagttg cggcaacgaa atcttcgagt cgcgccagat gacgcgggcg
     1201 cgttgcgcga ccgcattcac ctgcacgctc atcactccgg gcattgcgcc gagcgatcgt
     1261 tcgatgagcc agacgcaggc ggtgcaacgc acgccttcga ccagcaataa cgattcacgc
     1321 aggccgtcac ccaggtcgcg gatcacgtgg cgggcgttgt ccgtttgcca gtagtcgtgg
     1381 gtctccgggt ccgcatcgcg ggccggcttt tgcgcagccg ccgtacgcaa ggcgtagtaa
     1441 tcagagaggc cgagttgctc gatccactcg gccgcggcgc ggcaaccctg gcagcacatc
     1501 gggtgcgacg cgccagccac tcgtgcatgg atgacgacgc ccttctggag cgcttcgcca
     1561 cagtgccagc agttaggcga actcgacacg agtgtggctc atccggagct cgccgcgacg
     1621 atcagcgcaa tgcaaccggc gatggaggcg agcagaatgg cggtgcccag ccacatcacc
     1681 ggcggccgat accagggctc accatcgggt ttcatttggc cgccacttgc ggggccactc
     1741 gcctgccctg ggcaaggatc cacgcggtga tcagccgtgc ctcatcggcc gagagccgct
     1801 ggcgccaggg cggcatcacc ccgttgcggc cgtcgcgcac gctggccacg acgttttcga
     1861 tgttgccgcc ataaagccat gcgctatcga aaagattggg tgcacccatc gccggattcc
     1921 cgcggccatc ggcgccgtga caggcgacgc acagggcgtc gaaccgcacc ttgcccgcgg
     1981 cgacccagtc cggcgggacc tgcgtgccac tgacgctgac gacatacgcc gccacttcgt
     2041 ttgtgccgtt gtgaccgagc gcgccggcga gcggcggcat cacgccgttg cggccatcga
     2101 gaatgctggt gaggatggtc tcggcatcgc cgccgtacaa ccagtcggca tcggtgagat
     2161 ccggcgcacc cacggcttga ttgccctgcg cctcgcggcc atggcaggcc gcgcaattgt
     2221 cgagatacag gcgatgacca aagctcacgg cctctttgtg aaccgcgagc tcttcgaagc
     2281 tgagcccgcg caatggagcc aggcgcacct cgagcaatac gtcgttggcg gcagtgtggc
     2341 gttgcagctc gccctttgag gtccaggcaa gtcggcctgc gaaactgccg aatcccggat
     2401 acagagatag ataggcaacg ccgaacatga aggcgccgat cgatatgagc acccaccacc
     2461 atggcaacgg gcggaccgct tcgcgcagtg cgccgtgcgc ccacacgtga cccgtggtgc
     2521 cgtccgatgc cgtgggaatc cgcatccaca gcgcccacac gaacaacgcc acgctgatgc
     2581 caaccgtcac gcaggccagg actatcaccc aggcggacca gaagccgctc atggcgaatc
     2641 ctcgtcgagc ggcaaccgcg ccagttctga aaaatctttc tcatggtgcg ggagccacgc
     2701 ccagatccag atgccgatga atatcagcat gatcaccacg atcatcacgc cgatgaaatg
     2761 gccgtaaagc tcgctcatgg cgtcaccgca gcagtggccg gcacggcctt cacgcccaga
     2821 ccttgtagat aggcgatcag cgcgtcgagc tcggtcttgt cgagcacggc ctcggcagca
     2881 cccgcgatct gcgcatcggt gtacggatcg ccgagggtgc gcagcgtgcg catcttggcc
     2941 tgcaggtatt caccgtcgac cttggtttcc gccagccatg gaaaacccgg catgttcgac
     3001 cggggcacga gctggcgcgg cgccaaaagg tgcgcgcgct gccacgcgtc ggagtatttg
     3061 ccgcccacgc gcgccaggtc cggcccggtg cgcttcgagc cccactggaa cggtcggtcg
     3121 tacacgaatt caccggcttc ggaacgggcg ccgtagcgct gcgtttcgaa gctcagcgtg
     3181 cggatcatct gcgaatggca gaggtagcag ccctcgcgca cgtagacgtc ccggccggca
     3241 aggcgcagcg gttcatacgg tgtgacgccg ggagcgggcc tgacggtctg cgcgtcgttg
     3301 aacagcggga tgatctcggc gaggccaccg aaagaaacca cgaccgcgat cagcacgccg
     3361 agcaggagcg cgttcttctc gagtttttcc caatgtttgt agctgcccat gatcagaccc
     3421 tttccggcag cggcgccggg aactggtcgg gcacgggttc cggaatcgtt tcaggtgctg
     3481 gaaccaggat gggcttgatc acgccggcgc gcgcgttggc agcggtgtgc cacaggttcc
     3541 acgccatgac gaccatgccg ctgagcacca gcactccgcc caggaagcgc gccaggtagt
     3601 aaggctgggt ggcgatgatg ctcgacagga agtcgtgggc gagcgacccg tctgcgttcg
     3661 tctcgcgcca catcagtccc tgcgtgacgc ccgcggccca catggcgacg atgtacagga
     3721 gcagtccggt cgtgtgcagc cagaaatgca ggttcatcgc gggtgtctaa tgcatggccg
     3781 gacgatcgag cgcgcgtggc gccatcgcat acagcgaacc gatggtgatc at
//
LOCUS       DASQJS010000144        10740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1646142, whole genome shotgun sequence.
ACCESSION   DASQJS010000144 DASQJS010000000
VERSION     DASQJS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10740
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646142"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="VEW08_05905"
     CDS             <1..1793
                     /locus_tag="VEW08_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HYJ40304.1"
                     /translation="ILAWLVLCPIVSVSLAAPDFRARAPEDEVVYFVLPDRFENGDVN
                     NDLGGLQGDRLTTGFDPAHKGFYHGGDLKGLIARLDYIQGLGATAIWLGPIYKNKPVQ
                     GGPGHESAGYHGYWITDFLSVDPHFGNEADMKAFVAAVHARGMKVYLDIITNHTADVI
                     QYRECMGVACPYRSKGDYPYSRKGGAQGPAINAGFAGDGVRTAANFAQLTRDDFAYTP
                     FVAPSEANVKVPAWLNSTIYYHNRGNSEWWGESSLLGDFVGLDDLMTENPRVVQGFID
                     IYGEWIDRYGIDGFRIDTARHVNPEFWQSFAPAMLNRARTRGIPNFHIFGEVAAESVD
                     VAQLARHTRVDKIPSVLDFAFARAVEKVVAGKDGTDVLARLYQDDGLYEGGAQAALRL
                     PTFTGNHDFGRLAWMIRTARPGASDEEILKRAMLSNAMLFLLRGVPVVYYGDEQGFVG
                     HGIDQDSRQDMFASRVASYNDQALLGTSTTTAVANFNPQHPLYAQIAGLAKLRQSHVS
                     LRRGRQVVRAFARTPGVFAASRIGSDGREIVVAFNTSMETSVAQIEVEPNSKVFRTLR
                     GGCAAKASAPGSFRVEVPPLSFVVCEGMAQ"
     gene            1790..3892
                     /locus_tag="VEW08_05910"
     CDS             1790..3892
                     /locus_tag="VEW08_05910"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HYJ40305.1"
                     /translation="MRLRLALTLLLLCGSAAHAQVVASDATVRSPDGKMTVTMRVSGE
                     GRPEYSIQRNGRNVIDWSRLGFILADGPKLERNFELVQDDGKAIDETWEQPWGERRFV
                     RNRCTEQRVTIREKTGQRRAITVVFRVFDDGVGFRYEFPEQASLAQVGIVEELTEFAV
                     AEPATAWWIPGGEWNRYEYLYNRTPLTEVGQAHTPITLRTASGLHIAIHEAALVDYAG
                     MWLRRVSGQRLKAVLSPSSSGPKVSRKAPFATPWRTLQIADTAAGLYMSDLILNLNEP
                     NKLGDVSWVKPFKYVGIWWEMHLGTKTWSSGPKHGATTAETRRYIDFAAANGFRGVLV
                     EGWNKGWDGDWFAKGDEFSFTEAYPDFDIVALAAYAKKKGVRLVGHHETAGNIAVYEK
                     QLGAALDLYAKLGIDAVKTGYVADAGGVQALGDDGRIHFEWHDGQLQSRHHLRVVEEA
                     AKRHIAVNAHEPIKDTGLRRTYPNWVAREGARGMEYNAWGDPPNSPEHEVNLVFTRML
                     AGPMDYTPGVLSLTGAGGKRIQSTVARQLALYVVLYSPIQMAADLPENYAKYPKPFQF
                     IKDVPVDWSDTRVLNGEVGDYATIARKDRHSNDWYLGAITDEQPRTLQVSLGFLDDGR
                     KYYATIYRDGRNAGFEGDGQSLEIETRPVSRYDTLQLKLAPGGGQAIRISTKPASRGP
                     HSSPKPAPKKPQPEFDVE"
     gene            3889..5361
                     /locus_tag="VEW08_05915"
     CDS             3889..5361
                     /locus_tag="VEW08_05915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ40306.1"
                     /translation="MNASAFKPQVSFWQIWNMCFGFLGLQFGFALQNANVSRIFQTLG
                     ANIDDIPILWIAAPLTGLLVQPVVGYYSDRTWNGLGRRRPYFLAGAILASLALIAMPR
                     SPVLWMAAGMLWILDASINIAMEPFRAFVGDQLSEKQRPAGYLMQSFFIGVGSVVASM
                     LPWIFAHAGVSNVSTGEGAIPDTVRYSFEIGAVVLAVTMLWTIFSTREYPPGELHGFA
                     DATPDAAGVSPGSAARARSRGLAWFAAGLLGAYLVWRFALDKQLYLLCGGIAVWGLAL
                     LINGGSAGSSMFSILMRDIDNMPGRMRQLVPVQFFSWLALFAMWIYTTPAVARVHFGA
                     TDVVGQQFNEGANWVGVLFGAYNGFAALAAMVIPWMVRSFGINLSHLVNLLLGAVGLA
                     SFAVIRDPQWLLASMIGVGFAWASILSLPYALLAGSVPSRKMGVYMGIFNFFIVIPQL
                     VAATVLGTALKHLLGGQPVYVLVVGGVSFALAGVLALRVK"
     gene            5494..6012
                     /locus_tag="VEW08_05920"
     CDS             5494..6012
                     /locus_tag="VEW08_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40307.1"
                     /translation="MINRVLIAVLAMALTGHAVAGSIWKTGITEEDFQKKVDAGPALK
                     PLVKTHFDARPAGTKIEVYYKRFSIMHLAHNPDAPNAHHFIMGKPGEPSWGYVKIAEI
                     EIYRAMRADPMVIALMKKEAAAIGGDALTDCQRDPLLLEDSQTRNRQDWRATDFEVVG
                     YQFSCTIVRRKD"
     gene            complement(6060..7076)
                     /locus_tag="VEW08_05925"
     CDS             complement(6060..7076)
                     /locus_tag="VEW08_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYJ40308.1"
                     /translation="MHEKPTSFDIAFLAGVSQPTVSRALRGSPVVSLETRKRIEEIAR
                     QLNYRVDKNASNLRSQHSNTLALLLFEDPTPDDSQINPFFLSMLGSLTRASARKGYDL
                     LISFQQLSSDFCQDYEDSRKADGIILLGYGDYEEYRPRLDRLVEQGTHFVRWGPVLTD
                     EPGVSVGSDNAQGGFDVTRHLLAQGRRRIAFFGSATTHSPEFFDRYRGYERAMIEAQV
                     STSSALQVDAINLEESGFQAANELRARGVEFDAIVAASDLIAIGALRALQESGIDVPR
                     QVAVVGFDDIPAASLTNPPLTTVMQDTRRAGELLVETLLRQIAGEAASNSMIPTRLVV
                     RKSG"
     gene            complement(7185..8846)
                     /locus_tag="VEW08_05930"
     CDS             complement(7185..8846)
                     /locus_tag="VEW08_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase family protein"
                     /protein_id="HYJ40309.1"
                     /translation="MNTAAQRLTPPAVAPANPWWRGATIYQIYPRSFLDTNGDGVGDL
                     PGIIQRLDYVASLGVEAIWVSPFFKSPMADFGYDIADYCAVDAIFGTLEDFDRLIAKA
                     HGLSLKVIIDQVPSHTSDQHAWFEESRQSRTNPKADWYVWADAREDGTPPNNWLSIFG
                     GVAWTWEPRRGQYYLHNFLSEQPDLNFHNPEVRQATLDNMRFWLDRGVDGLRLDAINF
                     CFHDAKLRDNPARPRHLRKAKGVSADNPYGFQWHRFNNTQPEMLPFLEDIRRMLDEYP
                     DVVALGEISSDDSTATVAEYTQPGRLHMAYSFELLSNDSSPAHIRSTVETLRQRAPQS
                     WPCWTISNHDVERVVSRWGRSASAPHMATQLSCLVACLRGSVCVFQGEELGLAEADVP
                     FEALRDPYGIAFWPTFKGRDGCRTPMPWDASERAGFSSVPPWLPVPHQHLQLSVQAQE
                     NDKSSALQGFRRLLEWRKTLPLLINGDIEFLAATESVLAFRRFDSQGSMLAAFNLSGE
                     PASVALPGLAVRELIGGHGLPEGSFANATLHLPGHGVAFFTLAAP"
     gene            9126..10559
                     /locus_tag="VEW08_05935"
     CDS             9126..10559
                     /locus_tag="VEW08_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HYJ40310.1"
                     /translation="MRLNSDRARAFSGASIVLLALAAFAAAPLSRAADAASSRPAARQ
                     SPDWLRDAVVYEVFPRAFSKEGDFKGVTAQLDRLKDLGVTVLWLMPIHPVGKLKAKGT
                     LGSPYAVRDYDAINPEYGNGDDLKALVAGAHQRGMKVFIDIVANHTAWDSVLIGKHPD
                     WYTHDAGGRIVPPNPDWVDVADLDYSKPALRKYMSGMLVRWLRDYGIDGFRCDYASGV
                     PTDFWESVRPELDRVRPGLAFLAESDDPALLNKAFDIDYAWDFYHAMSEALVGRAPAS
                     SVRETWERAEAKYPRGALRLRFSDNHDQLRTTGAAGLPAALAASAVMFTLDGAPLLYN
                     GMEVGDGTESAAPGLFERAPIAWEMAERRPQVVPYYRALAALRRAHPALTRGAVHWLR
                     NGDEQRILTYERAGAGEALLVAVNLSSLPYAGKVEAPSGEYQEITPVLPAAAPVSPAQ
                     QDSKRAAKLPALVLAPWEFRVFRRAAP"
BASE COUNT         1928 a         3288 c         3569 g         1955 t
ORIGIN      
        1 gaattctcgc gtggctggtg ctgtgtccga tcgtctccgt ttcattggcc gcgccggact
       61 ttcgcgcgcg tgctcccgaa gacgaggtcg tctacttcgt ccttcccgac cgcttcgaga
      121 acggcgacgt caacaatgac ctcggcgggc ttcagggcga tcggctaacc accggcttcg
      181 atcccgcgca caagggcttc taccatgggg gcgacctcaa gggcctgatc gcaaggctcg
      241 actacatcca gggcctgggc gccacggcca tctggctggg gcctatatat aagaataagc
      301 cggtgcaggg cggccccggc cacgaatccg ccggctacca cggttactgg atcaccgact
      361 tcctgagcgt ggatccgcat ttcggcaacg aggccgacat gaaggccttc gtcgccgcgg
      421 tgcatgcgcg tggcatgaag gtgtacctgg acatcatcac caaccatacc gccgacgtca
      481 tccagtaccg tgagtgcatg ggcgtcgcct gcccgtatcg ctcgaagggc gactatccgt
      541 attcgcgcaa gggcggcgcg caggggccgg ccatcaacgc cggatttgcc ggcgatggcg
      601 tgcgcaccgc cgccaatttc gcgcagctga cgcgcgatga ctttgcgtac acacctttcg
      661 tggcgccctc cgaggccaac gtgaaggtgc cggcctggct gaactccact atctactacc
      721 acaatcgcgg caattcggaa tggtggggcg agagctcgct gttaggcgat ttcgtcggac
      781 tcgacgatct catgaccgag aacccgcgcg tcgtgcaggg cttcatcgat atttacggcg
      841 aatggatcga ccgctacggc atcgatggtt ttcgcatcga caccgcgcga cacgtgaacc
      901 ccgagttctg gcaatcgttt gcgccggcga tgctcaaccg agcgcgcacg cgcggcattc
      961 ccaacttcca catcttcggt gaggtggccg ccgagagcgt ggacgtggcg caacttgcgc
     1021 gccacacgcg cgtcgacaag atcccatcgg tgctcgactt cgcgttcgcg agagcggtag
     1081 aaaaggttgt ggcgggcaag gacggcaccg acgtgctcgc gcgcctgtac caggatgacg
     1141 gcctgtacga aggcggggcg caggcggcac tgcggctgcc cactttcact ggcaaccacg
     1201 atttcggccg gctcgcgtgg atgatccgca ctgcacgccc gggtgcgagc gacgaagaga
     1261 tactgaaacg cgcgatgctg tcgaatgcca tgttgttcct gttgcggggc gtgccggtgg
     1321 tgtattacgg cgacgaacag ggcttcgtgg gtcacggtat cgaccaggac tcgcgccagg
     1381 acatgttcgc gagccgggtg gcgagttaca acgaccaggc cttgttaggc actagcacca
     1441 ccaccgcggt ggcgaacttc aatcctcagc atcccttgta tgcgcagatc gcgggcctcg
     1501 cgaagctgcg gcagtcgcat gtgagcctgc ggcgcggtcg acaggtagtt cgcgcgtttg
     1561 ccaggacgcc gggggtgttc gccgcttcac gaatcggcag cgacggccgg gaaatcgtcg
     1621 tggcattcaa cacgtcgatg gagacttcag tggcgcagat cgaggtcgag cccaattcga
     1681 aggtattcag gacgctgcgt ggcggctgcg ccgccaaggc aagcgcgccg ggcagcttcc
     1741 gcgtcgaagt gccgccgctg agctttgtcg tgtgtgaagg aatggctcag tgaggttgcg
     1801 tctcgcgctg acgttgctgc ttctgtgcgg atctgccgcg catgcccagg tggtggcgtc
     1861 ggacgccacg gttcgctcac ccgacggcaa gatgaccgtc acgatgcgtg tcagcggaga
     1921 ggggcggcct gaatattcaa tccagcgcaa tggccgcaac gtcatcgact ggtcgcgcct
     1981 cgggttcatt ctcgcggatg gtccgaagct cgagcgcaat ttcgagctgg tgcaggacga
     2041 cgggaaagcg atcgacgaaa cctgggagca gccctggggc gagcggcgtt ttgtccgcaa
     2101 tcgatgcacc gaacagcgcg tcaccattcg cgagaagacc gggcagcgac gcgccatcac
     2161 cgtggtattt cgagtattcg acgatggcgt ggggtttcgc tacgaatttc ccgagcaggc
     2221 atcgctggcg caggtgggaa tcgtcgagga actcaccgag ttcgccgtgg cggagcccgc
     2281 caccgcgtgg tggattccgg gcggcgaatg gaaccgctac gagtatctct acaacaggac
     2341 gccgctgacc gaggtgggcc aggcgcacac gccgatcacc ctcaggaccg cgagtggctt
     2401 gcacatcgcc atccacgaag ccgcgctcgt cgactacgct ggcatgtggc tgcgacgcgt
     2461 gtctggtcag cgcctgaagg cggtactgtc accttcgtcg agcgggccga aagtaagccg
     2521 gaaagcgccg ttcgcaacgc cgtggcgcac gctgcagatc gccgacacgg cggccggact
     2581 ctacatgtcg gacctgatcc tcaacctcaa cgagcctaac aagcttggcg atgtgtcgtg
     2641 ggtgaagccc ttcaagtacg tgggtatctg gtgggaaatg cacctcggca ccaagacctg
     2701 gagctcgggg ccgaaacacg gcgcgaccac cgcggagacg cgccgctaca tcgatttcgc
     2761 ggcagccaat gggttccgcg gtgtgctggt ggaagggtgg aacaagggct gggatggcga
     2821 ctggttcgcg aagggcgatg agttcagctt caccgaggcc tatccggatt tcgacatcgt
     2881 tgccctggcg gcttacgcga agaagaaagg cgtgcgcctg gtgggtcatc acgagaccgc
     2941 cggcaacatc gcggtgtacg aaaagcagtt aggcgcggcg ctcgatctgt atgcgaagct
     3001 cgggatcgac gccgtgaaga ccgggtacgt agccgacgcc ggtggcgtgc aggcgctggg
     3061 cgacgatggc cgcatccatt tcgaatggca tgacggccaa ctccagtcgc gtcatcacct
     3121 gcgcgtggtc gaggaagcgg cgaaacgcca catcgccgtg aatgcgcacg agcccatcaa
     3181 ggacacgggt ctgcggcgca cctacccgaa ctgggtcgcg cgcgagggcg cgcgtggcat
     3241 ggagtacaac gcctggggcg acccgcccaa ttccccggaa cacgaagtga acctcgtatt
     3301 cacacgcatg ctcgcgggac ccatggacta cacgcctgga gtgctgagcc tgaccggcgc
     3361 gggcggcaag cgcattcagt cgaccgtggc ccggcagctg gcgctgtacg tggtgctcta
     3421 cagcccaatc cagatggccg ccgatttgcc ggaaaactac gcgaagtatc cgaagccgtt
     3481 tcagttcatc aaggatgtgc cggtggactg gagcgacacg cgcgtactca acggcgaggt
     3541 gggcgactac gccaccatcg cgcgcaagga ccgacacagc aacgactggt atctcggcgc
     3601 catcacggac gagcagccgc gcacgctgca ggtgtcactg ggcttcctcg acgacggcag
     3661 gaagtactac gccacgattt atcgcgacgg gcgaaacgcg ggcttcgaag gcgatggcca
     3721 gtcgctcgaa atcgagacgc ggccggtgtc gcgttacgac acgctgcaac tcaagctcgc
     3781 gccgggcggc ggccaggcga ttcgcatttc gacgaaaccg gcgagccgcg gaccacactc
     3841 atcgccgaag cccgcgccga agaagcccca accggagttc gacgtcgaat gaacgccagc
     3901 gcattcaagc cacaggtctc gttctggcag atctggaaca tgtgtttcgg attcctgggg
     3961 ctgcaattcg gattcgcgct gcagaacgcc aacgtcagcc gcatctttca gacgctgggc
     4021 gcgaatatcg acgacatccc gatcctgtgg atcgcagcgc cgctgaccgg gttgttggtc
     4081 cagcctgtcg tgggctacta ctcggaccgc acctggaacg ggctgggcag gcggaggccg
     4141 tacttcctgg caggagccat attggcgtcg ctggcgctca tcgccatgcc gcgctcgccg
     4201 gtgttgtgga tggcggcggg gatgctgtgg attctcgatg ccagcatcaa catcgccatg
     4261 gagccgttcc gcgcttttgt cggcgaccag ctctccgaga agcagcggcc cgccggctat
     4321 ctcatgcaga gctttttcat cggcgtgggt tcggtggtcg cgagcatgct gccgtggata
     4381 ttcgcgcacg cgggtgtcag caacgtgagc acgggtgaag gtgcgattcc cgatacggtg
     4441 cgctattcgt tcgagattgg cgccgtggtg ctggccgtca cgatgttgtg gacgatcttc
     4501 tcgacacgcg agtatccgcc cggcgagctc catgggtttg ccgatgccac gccggacgcc
     4561 gcgggggtga gccccggcag cgccgcccgg gcgcgttccc gcggattggc ctggttcgcc
     4621 gccggcttgc tgggtgcgta cctggtctgg cgcttcgcgc tcgacaagca gctctacctg
     4681 ctgtgcgggg gtatcgcggt atgggggctg gctctgctga tcaacggcgg cagcgctggc
     4741 agcagcatgt tctcgatcct gatgcgcgac atcgacaaca tgcctggtcg catgcgccaa
     4801 ctcgtacccg tgcagttctt ctcctggctg gcgctgttcg cgatgtggat ctacacgaca
     4861 cccgcggtag cgcgagtgca cttcggcgca acggacgtgg tggggcagca gttcaacgag
     4921 ggcgcaaact gggttggcgt cttgtttggc gcctacaacg ggttcgcggc actcgctgcc
     4981 atggtgattc cctggatggt gcgcagcttc ggcatcaacc tgagccatct cgtcaacctg
     5041 ctgctgggtg cagtggggct ggcatccttc gcggtgatcc gtgatccgca gtggctgttg
     5101 gcatcgatga ttggtgtggg ctttgcgtgg gcgtcgatcc tttcgttgcc gtacgcactg
     5161 ctggccggca gcgtgccctc gcgcaagatg ggcgtgtaca tgggcatctt caatttcttc
     5221 atcgtcatac cgcagctggt cgccgcgact gtcctgggaa cggcgttgaa gcacctgctc
     5281 ggcggccaac ccgtttacgt gctcgtggtc ggcggcgtga gctttgccct ggccggagtg
     5341 ctggcgcttc gcgtgaagtg aagcccaaac ccgggctttc tcgactatct acacacgcat
     5401 cggcgatgcg cccggatggt tgtgcgtggc tggcgctttg gcgatgctcg tgggagcatt
     5461 cgtgcggagg cggagagcgg ggggaggcag ctgatgatca atcgggtgtt gattgccgtg
     5521 ctggcgatgg cactgaccgg gcatgcggtt gccggcagca tctggaagac gggcattacc
     5581 gaggaagatt tccagaagaa ggtcgacgcg ggtccagccc tgaagccgct cgtgaagaca
     5641 cacttcgacg cccggcctgc tggcacgaag atcgaggtct actacaagcg cttcagcatc
     5701 atgcatctcg cgcacaaccc ggacgcgccg aacgcgcatc acttcatcat gggaaaaccc
     5761 ggcgagcctt cctggggcta cgtcaagatc gcggaaatcg agatctaccg cgcgatgcgc
     5821 gccgatccga tggtcatcgc gctgatgaag aaggaggcgg cggcgatcgg cggcgatgca
     5881 ttgaccgact gccagcgcga tccgctcctg ctggaagaca gccagactcg caacaggcag
     5941 gactggcgcg ccaccgactt tgaagtggtc ggctaccagt tttcctgcac cattgtccgg
     6001 cgaaaggatt gaaatttggg gtcaggcacc gttttccagg aaacggtgcc tgaccccatt
     6061 tagccggact ttcggaccac caggcgcgtg ggaatcatgc tgttgctggc cgcttcaccg
     6121 gcgatctgcc gcagcagcgt ttcgaccagc aattcaccgg cgcggcgcgt gtcctgcatg
     6181 acggtagtca ggggcgggtt cgtcaggctc gcggccggga tgtcatcgaa tcccacgact
     6241 gcaacctggc gcggcacgtc gatgccggat tcctgcagcg cgcgcaacgc tccaatggcg
     6301 atgagatcgc tggctgcgac gatggcatcg aactcgaccc cgcgcgcacg cagctcgttg
     6361 gccgcctgaa agcccgactc ttcgagattg atggcatcca cctgcagcgc ggatgacgtg
     6421 gaaacctgcg cttcgatcat ggcgcgctcg taaccacgat agcgatcgaa gaactccggc
     6481 gagtgggtgg tggcgctgcc gaagaacgcg atcctgcggc gaccctgcgc gagcagatgg
     6541 cgcgtgacgt cgaagccgcc ttgcgcattg tccgacccga cggacacgcc gggctcgtcg
     6601 gtgagcaccg gtccccagcg cacgaaatgc gtgccctgtt cgaccagccg gtcgagccgc
     6661 ggacgatatt cctcgtagtc gccgtagcct aacaagatga tgccgtcggc ctttcgactg
     6721 tcttcgtagt cctggcagaa gtcgctggag agctgctgaa acgaaatgag caggtcgtaa
     6781 cccttgcgcg ccgaggcgcg tgtaagcgag cccagcatcg acaggaaaaa cggattgatc
     6841 tgcgagtcgt cgggcgtcgg gtcttcgaac agcaacagcg ccagcgtgtt cgagtgctgc
     6901 gagcgcaggt tggacgcatt tttgtcgacg cggtagttga gctggcgggc gatctcctcg
     6961 atccgcttgc gcgtttcgag actgaccacg ggactgccgc gcaaagcgcg agacacagta
     7021 ggctgtgaga cgccggcgag aaacgcgatg tcgaacgatg tcggcttttc gtgcattgat
     7081 cgcggcgctt ttttgagcgc cgtcccctcg agtggcgcga tcatgcatgg gcccatcggg
     7141 gaaaagctac tgcaccggcg agatgtaaaa cacaggcagc aaaatcaagg cgctgcgagt
     7201 gtgaaaaagg ccacgccgtg cccgggcagg tgcaaggtag cgttagcaaa actgccttcc
     7261 ggaagtccgt ggccaccgat gagctcgcgc accgcgaggc cgggaagggc gacgcttgcc
     7321 ggctcacccg agagattgaa tgcggccagc attgaaccct gcgaatcgaa gcggcggaac
     7381 gccaacaccg attcggtggc cgccaggaat tcgatatcgc cgttgatcag cagcggcagc
     7441 gtcttacgcc actcgagcag tcgccggaag ccctgcaatg ccgacgactt gtcgttctcc
     7501 tgggcctgga ccgacaactg caggtgctgg tgcgggacgg gcagccacgg cggcacgctc
     7561 gagaacccgg cgcgttcgga ggcatcccag ggcatgggcg tgcggcagcc atcgcgtccc
     7621 ttgaacgtgg gccagaacgc gattccgtac ggatcgcgca gcgcttcgaa aggcacgtct
     7681 gcttccgcga gcccgagctc ttcgccctgg aacacgcaca ccgaaccgcg caggcaggca
     7741 acgaggcacg acaactgcgt ggccatgtgc ggcgcggagg cgctccggcc ccagcggctg
     7801 accacgcgct caacatcgtg gttcgagatc gtccagcaag gccagctctg cggagcgcgt
     7861 tgcctcaagg tttcgacggt ggatcgaatg tgcgccgggg agctgtcgtt gctcagcagc
     7921 tcgaagctgt aggccatgtg cagccgtccc ggctgcgtgt actcggccac cgtcgccgtg
     7981 gaatcatccg acgagatttc ccccagcgcg accacgtcgg gatactcgtc gagcatgcgc
     8041 cggatgtcct cgagaaaggg caacatttcc ggctgtgtgt tgttgaagcg gtgccactgg
     8101 aatccgtagg gattatcggc gctgacaccc ttggccttgc gcaggtgcct gggccgcgcc
     8161 gggttgtcgc gaagcttcgc gtcgtgaaaa cagaagttga tggcatcgag gcgcaggccg
     8221 tcgaccccgc ggtcgagcca gaagcgcatg ttgtcgagcg tcgcctgccg cacttccggg
     8281 ttatggaagt tcaggtccgg ctgttcactc aggaaattgt gcaggtagta ctgcccgcgg
     8341 cgaggctccc aggtccacgc gacgccgccg aagatcgaca gccagttgtt aggtggcgtg
     8401 ccgtcttccc gggcgtccgc ccagacgtac cagtcagcct tcggattggt gcggctctgc
     8461 cggctttcct cgaaccaggc gtgctggtcg gaggtgtggc tcggcacctg gtcgatgatg
     8521 accttgagcg acagcccgtg cgccttggcg atgaggcggt cgaaatcctc gagcgtgccg
     8581 aagatggcat ccaccgcgca gtagtcggcg atgtcgtagc cgaagtccgc catgggcgac
     8641 ttgaagaacg gcgaaaccca gatggcttcg acgccgagcg acgccacgta atcgaggcgt
     8701 tgaatgatac cgggcaggtc gccgacgccg tcgccgttgg tgtcgaggaa gctgcgcgga
     8761 tagatctggt agatagtcgc gccgcgccac caggggttgg ccggcgccac ggcgggcggg
     8821 gtcagtcttt gagctgctgt gttcatgcgc gcggtgcggg agcttaccgt agacggggaa
     8881 acactctcac aagtgcatac gtattcagcc tgaatacgta tgcaagccgt tgtttgtgca
     8941 ccagcgtggc tcctaccatg acccgctctc gatagattga acgtctgtat gacttttgcg
     9001 ttaggcctct ccggctgccc ccctcggccg gcgaggcttt cttttatctg catacgtatt
     9061 caacgggagc gccttccggg cgctgctaat gtcgccgcga ttcgtcaccg gctgagggag
     9121 gctggatgcg attgaattcc gatcgggcgc gcgcattttc cggtgcgtcg attgtcttgc
     9181 tggcgctggc cgcgtttgcc gcggccccgc tttctcgcgc cgccgacgcc gcaagcagcc
     9241 gtccggccgc gcgccagagt cccgactggc ttcgcgacgc ggtggtctac gaagtgttcc
     9301 cgcgcgcgtt ctcgaaggaa ggggacttca aaggggtcac cgcgcagctc gatcgcctga
     9361 aagatctcgg cgtgaccgtg ctctggctga tgcccatcca cccggtgggc aagctcaagg
     9421 ccaaaggcac gctcggcagt ccctatgccg tacgcgatta tgatgcgatc aatcccgaat
     9481 acggcaacgg cgacgatctg aaggcgctcg tggccggcgc gcaccagcgc ggcatgaagg
     9541 tgttcatcga catcgttgca aaccacaccg cgtgggattc ggtgctgatc ggaaaacatc
     9601 cggattggta tacgcacgac gcgggcggcc gcatcgtgcc gccaaatccg gattgggtcg
     9661 acgtcgccga cctcgactac tccaagccgg cgttgcgcaa gtacatgagt ggaatgctcg
     9721 ttcgctggct gcgcgactac ggcatcgacg gttttcgttg cgactacgcg agcggcgtgc
     9781 cgaccgactt ctgggaatcg gtgcggccgg agctcgaccg cgtgcgcccc gggctcgcgt
     9841 tcctcgcaga gtccgatgat ccggcactgc tcaacaaagc attcgacatc gactacgcct
     9901 gggacttcta tcacgcgatg agcgaggcgc tcgtgggacg cgcgccggct tcatccgtgc
     9961 gcgagacctg ggagcgcgcc gaggcgaagt atccgcgtgg cgccttgcgc ctgcgcttct
    10021 cggacaacca cgatcagctc cgcaccaccg gggcggcggg tcttccggcc gcactggctg
    10081 cttccgcggt gatgttcacg ctggacggcg cgccgctgct ctacaacggc atggaagtgg
    10141 gggacggcac tgaatcggct gcgcccggcc tcttcgagcg cgcacccatc gcctgggaga
    10201 tggcggagcg gcgcccgcaa gtggtgcctt actaccgcgc gctggcggca ctgcgccgcg
    10261 cgcatccggc attgacgcgt ggtgcggtgc actggctgcg caacggcgac gaacagcgga
    10321 ttctgaccta tgaacgcgcg ggcgccggcg aggctctgct ggtcgcagtc aacctgtcgt
    10381 cgctgcccta cgcgggcaag gttgaggcgc cgtcgggcga gtaccaggag atcacgccgg
    10441 tactgccagc cgccgccccg gtatcaccgg cacaacagga ttcgaaacgc gcggcgaagc
    10501 tgccagccct ggtgctcgcg ccgtgggaat tcagggtttt ccggcgggcg gcgccgtgaa
    10561 tacgtatgca tgcgattgcc aagtgtgtgc cgctggcaac atccttgcgg ccaggggttc
    10621 tcacgaaagt ttgggatgtc gcgcgctgtc ggcagcggcg atgcatcaac attagttagg
    10681 agaggggacg aatgaagaca ctaaagaaga agggcaagag ctccaagcct gcccagcgca
//
LOCUS       DASQJS010000145        20776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1331221, whole genome shotgun sequence.
ACCESSION   DASQJS010000145 DASQJS010000000
VERSION     DASQJS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20776
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331221"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VEW08_05940"
     CDS             <1..420
                     /locus_tag="VEW08_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40311.1"
                     /translation="PLISVINGAEKAFGARLEVTLPAEVTLVSVSASNATCSGTRVLS
                     CDFTELDPLASATVSLNVRGTATGNFVSALKLTASNDNNTANDSREVAVDIAGGDPAA
                     VSDGGRSGGGGRMEWLALLLLGAMAWRRTKMGSGTLS"
     gene            802..3387
                     /locus_tag="VEW08_05945"
     CDS             802..3387
                     /locus_tag="VEW08_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ40312.1"
                     /translation="MHKKRWTANSWLLPLACTPALLHAQDADTAATASTTASAAMTME
                     ASEVTETVIVTGTRATGVDTFSSSSPIQVLDADELQSAGKPDLMNALANMVPSFTAQA
                     FGGDMANQTLQAKMRGLSPNHTLVLVNGKRRHTTGSLAILGGPYQGGAGVDLNFIPVD
                     SIDHVEVLTEGAAAQYGTDAIAGVINIIMKKKPSGGSLSVSQGGNYAGDGETSQFSAN
                     FGLEANSGSYINFAAEYREHEHTNRGDIDPRVVDPANVDPDEGTFPNTNMPFAPGYPY
                     LNQIFGDAAYEIKLLSINAGIPIGDDTEIYATTTVGEKHAASFENYRLPSRVSYTDPD
                     TEEVTYFRPYGFSPREETEETDFSVGTGIKGEVGTWNWDVATTYGKDDIDMFTRDSAN
                     ASLYANTGQTPVNFYDGTYTATQWTTNADLSKEIDIGLEEPMNLAFGAEYREETWEAA
                     PGDEGSRYFEGGQSFPGISLSDAGAHDRDVVAAYLDVVVEPLSGLRLDLAGRYEDYSD
                     FGDATVGKLSGRYEITPSFALRGTVSNGFRAPTLAEEYYSATNVGPTTAFVQMPPNAE
                     ATALLGLGAGLQPEKSTNYSLGFVWRSEKAIGLSFDIYQVEVRNRIAATSTFYGTING
                     ELYSQAIVDAIIANGNVLDPAVVEEGDTGINLFTNGVTTETRGAELMFDYNTDLGSAK
                     TTFSVTATYNETEVTKVRETPPELGTQPLFDKVALSDLQDTAPKYLLNFGAVFEWDRV
                     TFSIHEMIYGESSQFENDGGGTPNPPPAGAPEPDPSGLRFYQTEIGVTPITNFEVTFQ
                     AMDALAITVGATNAFDEYPEKRNDMLREIQFNNNDNSAVAGYPSFSPFGINGAYYYGK
                     LVYSF"
     gene            complement(3455..3970)
                     /locus_tag="VEW08_05950"
     CDS             complement(3455..3970)
                     /locus_tag="VEW08_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYJ40313.1"
                     /translation="MTRQHRSSHVRAAVTCALGFASIGAAHAADVVRHPLPGGSTFPI
                     ARAVEVPAGKTIIFHSGTTPAPLDPKAEAGTAAYWGDTKTQALSTFARIKESLDAMKL
                     DFGNVVAMTVYLVGDPAKGGRMDFEGFMAAYTQFFGTKEQPKLPARSTVQVAGLVAPG
                     MLVEIEVQLVK"
     gene            complement(4075..4677)
                     /locus_tag="VEW08_05955"
     CDS             complement(4075..4677)
                     /locus_tag="VEW08_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYJ40314.1"
                     /translation="MKRRNALAAGIALALIAGSASAGNDFDYCLLCHGTNANGNYGIR
                     APKLSGMEPWYLARQLENFASGARGTPAEDAPGHEMGPVGMRLKAEGTLDAAVQFIGK
                     LDSKRPAPTITGDVAHGKKLYENCASCHGAKGEGNASLNSPALAARSDWYLVAQLVNY
                     QKGLRGTDERDTYGAQMRAIVTTLPDEKAIKDVVAYINTL"
     gene            complement(4674..6242)
                     /locus_tag="VEW08_05960"
     CDS             complement(4674..6242)
                     /locus_tag="VEW08_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HYJ40315.1"
                     /translation="MTNKLSRRGFLNMAGAVGGSTTVYRAALALGLLPAIAHAERPEL
                     GTLRKPKKVLILGAGISGLTAAYELSRKGYDVQVLEASFRAGGRNMTLRNGDRIDETG
                     FPQVCNFDKDPDLYFNCGPARIPGHHKALLNYCKELEVELAPFINDNRLAWVQDDAMF
                     GGKPVRAREYMTDSRGFVAELMSKSMTPEQFAAPMTKDDYERLREYVRFLGNLDPSFK
                     YAGSSNAGLAVHDYTNPDVLKKPLDGRELLKSRYMMIMNFGETDDQAAMMMEPVGGMD
                     RIPAGFLRKIGHLVQLESQVQSIKVLPKGVEVTYRRNGEYVRAKADYVLNCIPMHLLA
                     GIENNFPKEYVAGFTAIPRGKLFKIGFQMKERFWEKEGIYGGISWTLQDIQQLWYPAH
                     GINRQKGVVLGAYTFDPNAGEKFARLPPAERFKLAIAQGEKIHPGYGSYVETGVSVAW
                     HQMNHMLGCAAQWDEELWKKWFKTLQTPVGNHYLMGDQVSYHPGWQEGAMASAHHAIA
                     NIDARERAAGAVAA"
     gene            complement(6866..8425)
                     /locus_tag="VEW08_05965"
     CDS             complement(6866..8425)
                     /locus_tag="VEW08_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40316.1"
                     /translation="MKRTAWLLVTLALPALVVASPNGGPLGDFEAHADVGSPKIAGYS
                     TWNAASQVYTLSAGGVNIWAKRDEFQFAYRRMKGDFIVQAQVEFQGKGVDPHRKAGIM
                     VRTSIADFDSAYVDGALHGDGLTSLQFRKAKGEDTGQTAMPAAKGANVIQLERRGNVF
                     IFSAAKFGEPFEVAEIKDVPNVSEDAYVGLFLSSHNPDVKETVVFRHVRVIKPVKVGF
                     QPYRDYIGSRLEVMNVTSGHRNVYYTSRVPFEAPNWLPDGSALIYNASGNDPATRGRL
                     WKFDLATRTPTLIDTGFAIRNNNDHVLSFDGTQLAISDQSTGGGQSTIFTIPTRGGIP
                     KRITPLTPSYLHGWSPDGKWLTYTAGRPTKKDPKVLEWDIHKIASDGSGKEINLTNSP
                     GLDDGPEYSPDGQWIYFNSTRSGQMQIWRMKPDGTAQERVTRDDKFNDWFPHISPDGK
                     WIVIISYGLDVAAADHPYYKHCYLRIMPTDGSAPPKVIGYVYGGQGTINVPSWSPDGT
                     HIAFVSNSDVL"
     gene            complement(8574..9659)
                     /locus_tag="VEW08_05970"
     CDS             complement(8574..9659)
                     /locus_tag="VEW08_05970"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HYJ40317.1"
                     /translation="MKFFKTSMIAFAFVFASAAGAQDKYFTDWPAGTAPAEVGKRVAE
                     RFIPTPNMEMNSHGPQALHYSHVATWTGALQFANLTKDADLKKRLVDRFDPFLTANGT
                     LVPRADHVDGTVFGSLPLELYMQDARFRYRVMGLAFADAQWDRPLPDGLSYQTRWWID
                     DMYMITALQLAAFRATADSRYLERATRSMVAYLAKLQQPNGLFFHAPDVPFYWGRGDG
                     WVAVGMAELLRDLPPKHKSYKTVLSAYRRMMATLLTHQADNGMWRQLIDHPESWEETS
                     STAMFSYAFVTGVKNGWLAEDTYGPAARKAWIALVGYLTPEGLLREVCVGTGKRNDLQ
                     HYLNRPRVTGDTHGQAPMLWTATALLR"
     gene            complement(9656..10867)
                     /locus_tag="VEW08_05975"
     CDS             complement(9656..10867)
                     /locus_tag="VEW08_05975"
                     /inference="COORDINATES: protein motif:HMM:NF027479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HYJ40318.1"
                     /translation="MKLISLGVALAALSSNVFAAGATIAVTHDLGAARPREIISVPFT
                     QVAAVMPEARMYHLVVRDSKGRVIPSQITNYEHDHRGASYDELVFTYDFAAGEKRATF
                     TLENLSTASAPLTPCVYARLVPERFDDMAWENDRIAHRMYGLALNSPGAGGERLRGSG
                     IDVWAKRVSYPILDRWYAKGHDQFHKDEEGEGLDLYSIGGSRGAGGTGVWDGAKLWTS
                     DNFVNAQVLSNGPQRAAFKLSYAPWDVGPAGQVVETKRFTVDCGRNFDAVESVFDFPA
                     NESMVGIGISEHPQAAGFPAAVLTRDPDGRWMSFWEENKDGGLGIAVILANDATPAGF
                     AHEDAPGGKGNANNLLLVKAIDGVPLRYFTGAGWTKSGQFEGRVAWETYVKDFAARAQ
                     KPLVISVSASK"
     gene            complement(10952..12202)
                     /locus_tag="VEW08_05980"
     CDS             complement(10952..12202)
                     /locus_tag="VEW08_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HYJ40319.1"
                     /translation="MMRKVLFGAAVAATLLGGCGGGGDEPFTPPPGGGGPPPTGLPSP
                     GSAPALKTVLAAKYGVVNFPIGAAIEPASTTNADSALLLKHFSSITAENAMKPLSIWP
                     NQPGSSPAQPAPTPNFTQADIIADFAVANGIRLRGHTLLWHRSAPTWLFVGDLNNPPS
                     YRASVQQHLRSYITAVMQRYPEVYAWDVVNEAATDTPNATNPYRQDSVWYQAYLLAGR
                     PGGEYVIDAFRIAGEVRAQLGNPGMKLMLNDYNTELPGKRANVMSIVQDLINASVPID
                     GVGHQFHLQLGADVAQVTAAFTAVEAMSSTLVNHVTELDVSIYADPGTCYSQQTIPPC
                     LADLGANPPQSVLSQQATLYRALFNAFNRPSVQSVSFWGIADNHTWLNTYPVARTNRP
                     LLFDTARDPKWAFWTVVDPTIVVP"
     gene            12360..14312
                     /locus_tag="VEW08_05985"
     CDS             12360..14312
                     /locus_tag="VEW08_05985"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HYJ40320.1"
                     /translation="MLQRMAGRLALGMVVLAAACTSKAEEPLMSGIFVEHAVLQRDRP
                     IAVFGRAAAGEEVSVTLAGASASAKADAKGAWSLTLPAMSAGGPHTLTARSASRTQTA
                     SDVLVGDVWLCSGQSNMEWPVRATLDAGSEVALSANDRIRQVTIARAISAAPRTDFDQ
                     VLEWKVAGPATTEHFSATCYYFVRELQKTVNVPQGIISSNWGGSRIEPWMSEQALRGV
                     DGYQAQLDLLGQYRSQRPLAFQEWGENWQKWWLGQHAVTRGTQPWAVKGDSTAWQPAP
                     AALGYWENWGVPALGRYDGLMWFRAHVKLSKAQAQEAERLSLGPVDDVDVTWVNGRPV
                     GNGFGDEARVYALPPSVLKAGDNLVVVNVHDFWGNGGFHGSADQRALLLADGTRVPLT
                     DWEYSVAPSGLWPPHAPWESLSGVSVLYNAMIAPLGKYGLRGVAWYQGEANAGLDDAR
                     KYQALLAAWMADWRRQFDAPLPFLIVQLANFGPLSKMPVDSGWAQLREAQRRAAVADG
                     NAAIAVTIDIGNRDDIHPANKQDVGRRLARAARQVVYGEKISAYGARPRSATRAGGEV
                     AVTLDDFDGNLLVVGAKDPTGFELCGAAQESCRFVRAQLAGGGKLLLADAAAATRVRF
                     CWADSPLCNLYDSTGLPVGPFEMAIQ"
     gene            complement(14330..15421)
                     /locus_tag="VEW08_05990"
     CDS             complement(14330..15421)
                     /locus_tag="VEW08_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYJ40321.1"
                     /translation="MSETDRRQFLTRTAALAALPLLSSIGNTQAAGRKLGVALCGLGS
                     LSTNQIAPALQKTRNCRLAGIVTGTPSKAVEWQKKYGLSDKSVYSYQNMAQMASNPDI
                     DIVYVVTPNALHMEHTLAAAAAGKHVFCEKPLEISVERGQRMLDACKKAGKMLGVGYR
                     LQFDPNHLECIRLAREKVLGDVKIIDANFGFPIGDPKQWRLNRALAGGGSLMDVGIYC
                     LQTTRMLTGEDPIWVNATEIKTDTVKFKDVDETILWQSKFPGGVVANCVSTYSASGLA
                     GFRAATTRGWFAMDPAYFYNGNRGKRSDGTEINFPVADQFATELDDFADCIINKRPTK
                     VSGEMGLADVRFLMAIYESIKKGRPVSLA"
     gene            15575..16423
                     /locus_tag="VEW08_05995"
     CDS             15575..16423
                     /locus_tag="VEW08_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40322.1"
                     /translation="MTIQFNELLPSRRILGLSVVLALVSCTAGDGTGLDAGGRPIPPG
                     PPANNDFQQIQDTIFGPICSGCHQGANAPQGLRLDAGNSYALLVNVASAEVPGLMRVN
                     PGNPDTSYLVQKIQGNAAVGARMPANGPPYLSQTQIDLVRGWIAAGAPQSAAPADQLV
                     VVSSIPAVSEQAGAGLGKLTIIFNGDVDSSLANGNAFELRDANDRLVNAAVRVPSGRP
                     NVVEITTPQPLAAGSYQLAILGDGPSPLADQSGHVLDGDADGKPGGDTLIPFDVKAIA
                     KEGSVR"
     gene            16420..17511
                     /locus_tag="VEW08_06000"
     CDS             16420..17511
                     /locus_tag="VEW08_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40323.1"
                     /translation="MMRLTTLRLAGALALVAFASAAQAEPYLAAQMGLKCVQCHVNPT
                     GGGMRTVFGNAFAQTQLAAKRLGDEGDMWTGQVMKFLSVGGNARANYNFTDVPNQESS
                     NDFAVEEARAYLDFGVIPNRLSVYIDQRFAPGNSTNLEANIRYWISENSVYVKAGRMY
                     LPFGYRFEDDNAFVRQLSGINMQAPDEGVEIGFEKGSWSTQFAISNGTAGAPEVDSGK
                     QLTARTEYVGSAWRAGVSAVANDTDAGDRMGGSVFGAYRLGPVTLLGEVDYFDDDSIG
                     VEGRKLMASLVEADWKLRQGHNLKLTYEWLEPDDDVDEDEQTRTSLAYEWSPIQFVQL
                     RGGVRFYDGIPQSDLQNRTQAFLQLHGFF"
     gene            complement(17516..18535)
                     /locus_tag="VEW08_06005"
     CDS             complement(17516..18535)
                     /locus_tag="VEW08_06005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYJ40324.1"
                     /translation="MDISVVIPTRNRPGRVRNALSSLAQQTSPPAEVIVVDASDEGFH
                     DAKALASEFHTLPLILLSSAPSVCIQRNKGIEAAKSDWIFLCDDDIELPRDHLQHLAN
                     HVAKHPGCGAVAGRLRQLESGVWVDQYPPRRFSTALYHFVFQLSVWGPIDGVKTNRFT
                     RPVLAWMKEWYRKRGNRESLAGWPLITQWQRPFLTTRFYSLGADLVRREFLLSSPYDP
                     VLDPGGIGDNYGVALGLPNGIDVIDSTYALHHREQDNRVNRKVAYYRRILALHYFVRA
                     RFSNATITTLWLCWSIIGLSLLQLRHLDVSLLKLSVKAFFQIVAGRNPYVVAKNEGRI
                     VISPS"
     gene            18774..19571
                     /locus_tag="VEW08_06010"
     CDS             18774..19571
                     /locus_tag="VEW08_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ40325.1"
                     /translation="MDKPVQSQPAAFDSMAKGYDAEFTASVLGRVLRPMVWERFAACF
                     APGSSLLELGCGTGEDAIHLAKSGHRVLATDASAQMVDCARRKAEEAGVADRIRFQVV
                     PMESLSAVLGTEVFDGVYSNFGAVNCAPDLAALAGVLAPRLAPNAPLVWVIMGRFVPW
                     EMAWYLLRGDRRRAFRRFHRGGSEWRGLTIHYPTPGNCARRLEPHFVVNVARPLGVIL
                     PPSYAAGWLERTPRMRAWLSRLERTLFERALGAGLSDHYVLEARRAA"
     gene            19568..>20776
                     /locus_tag="VEW08_06015"
     CDS             19568..>20776
                     /locus_tag="VEW08_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40326.1"
                     /translation="MKTFRAFWTLLPPQLRRRCLALFGLALAMAATTVAGLVSVIPFF
                     QLLSDSEATPTTRWLHGIRATLGIADERVFLAATGVVFVLTLIVANVINYFGTRSIHS
                     FALGAADAIRVRLFGVYLRSDYLFHTRHDGARLGNDVLHESDRIIVLAAVFFQAVTAT
                     GVVVLIAVSLIIMNPWVLLGTLLVFGSAYALIFARSRRQLRVNSIELTRESARRAMVV
                     RQAFAAIKELLVMRLDAPFQRQFGEATARVSRSLAHTNAIGLRPKYVLECLAAFGLVT
                     VALVLHVQEGAPWLPQLTFLALCAYRLLPSLQQLFVGLVAVRAHRDVLFRMLKSVEEE
                     PASGGAVSVPKLEGAPARDIVFEDVTFRYGAEAPAVFEHLNLRIRAGEAIGVTGQNGS
                     GKSSFADLLLG"
BASE COUNT         3702 a         6390 c         6720 g         3964 t
ORIGIN      
        1 ccgctgatct cggtgatcaa cggcgcggag aaggcgttcg gtgcacggct cgaagtgacg
       61 ctgcccgccg aggtcacgct ggtgagtgtg tcggcgtcga atgccacctg cagcggcaca
      121 cgtgtgctga gctgcgactt caccgagctc gatccgctcg cgtcggccac cgtgtcgctg
      181 aacgtgcgcg gcaccgccac cggcaatttc gtgagcgcac tcaagctcac tgccagcaat
      241 gacaacaaca ctgccaacga cagccgcgaa gtggccgtcg atatcgccgg aggcgatccg
      301 gcggcggtaa gtgatggtgg ccgcagcgga ggcggcggcc gcatggagtg gctggccctg
      361 ttgttgctcg gcgcgatggc ctggcgtcgc acgaaaatgg ggtcaggcac cctttcctag
      421 gaaacggtgc ctggcaccgt ttccagtgag cgtccccatt ttcgtgcagg cgcttcacgc
      481 gccgttctcg tgctttcacg ctcctcgctt gcaccacgaa agcaaccttc ggattgtttt
      541 tgaggggtcg tgcgttttta tccacgcccg caatcgctga aattcttccg cgatctgatt
      601 gcggcgaacg aatccaactc cgcgtgatca cgcgcaattt ccaaggttgt taggccgcgc
      661 atcgcggcgc gcggcgcgcg tgcgtccggc ggctggccgg attattgcaa cactcaacta
      721 cagcaagaat gaccgggtga ttgcctgctc catatgcggg cagtcagatg aataaaactt
      781 gatacccagg gagatttttt catgcataag aaacgttgga ccgccaacag ctggctgcta
      841 ccactggcct gcacgccggc gttgctacat gcacaggatg cggacaccgc cgccacagct
      901 tcgaccaccg ccagcgccgc gatgacgatg gaggcgtcgg aagttaccga aaccgtcatc
      961 gtcacgggca cgcgcgccac gggcgtcgac acgttcagca gctcatcgcc gatccaggtg
     1021 ctggacgccg atgaactgca atcggccggc aaacccgacc tcatgaacgc actggccaac
     1081 atggtgccgt cgtttaccgc gcaagcgttt ggcggtgaca tggccaacca gacgctgcag
     1141 gcgaagatgc gtggcctgag ccctaaccac acgctcgtac tggtgaacgg caagcgccgc
     1201 cacaccacgg gcagcctcgc gattcttggt ggcccctacc agggcggcgc cggtgtcgac
     1261 ctcaacttca ttcccgtcga ctccatcgac cacgtcgaag tgctcaccga aggcgcagcg
     1321 gcgcaatacg gcacggacgc catcgccggc gtcatcaaca tcatcatgaa gaagaagccg
     1381 tccggcggca gcctcagcgt ctcgcagggc ggcaactacg ccggcgacgg cgagacgagc
     1441 cagttctcgg ccaacttcgg gctcgaggcc aacagcggct cgtacatcaa cttcgcggcc
     1501 gagtatcgcg agcacgagca cacgaaccgc ggcgacatcg atccgcgcgt cgtcgatccg
     1561 gcgaacgtcg atccggatga aggtacgttc ccgaacacga acatgccgtt cgcgccgggc
     1621 tacccgtatc tcaaccagat ttttggcgat gcggcttacg aaataaaact gctgtcgatc
     1681 aatgcgggca tccccatcgg cgatgacaca gagatctacg ccaccacgac cgttggcgag
     1741 aagcatgccg cgtcgttcga aaactatcgg ctccccagcc gcgtctcgta taccgatccg
     1801 gacacggagg aggtcaccta cttccgcccg tacggtttca gcccgcgtga agaaacggag
     1861 gagacggact tctcggtcgg caccggcatc aagggcgaag tcggcacctg gaactgggat
     1921 gtcgcgacga cctacggcaa ggacgacatc gacatgttca cgcgtgactc ggccaacgcg
     1981 tcgctgtacg cgaacaccgg gcagacaccc gtgaacttct acgacggcac gtacacggcc
     2041 acgcaatgga ccacgaacgc ggatctctcg aaagagatcg acatcggcct cgaggagccg
     2101 atgaacctcg cattcggcgc agagtatcgt gaggaaacct gggaagccgc tcccggcgat
     2161 gaaggctcgc gatacttcga aggcggccag tcgttcccgg gcatcagcct gtcggatgcc
     2221 ggcgcgcatg accgcgatgt cgtcgctgct tacctcgacg tggtggtcga gccgctgtcc
     2281 ggcctgcgcc tggacctcgc cggccgctac gaggactaca gcgatttcgg tgatgccacg
     2341 gtgggcaagc tgagcggccg ttatgaaata acgccgtcgt tcgcgctgcg tggcacggtc
     2401 agcaacggat tccgtgcgcc gacactggcc gaagagtact actccgcgac caacgtggga
     2461 ccgaccacgg cattcgtgca gatgccaccg aacgcggagg ccacggccct gctgggactg
     2521 ggcgccggcc tgcagcccga gaagtcgacc aactactcgc tgggcttcgt ctggcgcagc
     2581 gagaaggcga tcgggctgtc tttcgatatc taccaggtgg aagtgcgcaa ccgcatcgcg
     2641 gcgaccagca cgttctacgg caccatcaat ggcgagctct actcgcaggc catcgtcgac
     2701 gccatcatcg ccaacggcaa cgtgctcgat ccggcggtcg tggaggaagg cgacacgggc
     2761 atcaacctgt tcaccaacgg tgtgaccacg gaaacccgcg gtgcggagct gatgttcgac
     2821 tacaacaccg acctgggcag tgcgaagacc acgttcagcg tgacggcgac ctataacgaa
     2881 accgaagtga cgaaggtgcg tgagacgccg cccgagctgg gaacgcaacc gctgttcgac
     2941 aaggtggcgc tctcggatct gcaggacacg gcgcccaagt acctgctgaa tttcggggcg
     3001 gtgttcgagt gggaccgcgt cacgttcagc atccacgaga tgatttacgg cgagtcatcg
     3061 cagttcgaaa acgacggtgg tggaacgccg aatccgccgc ccgcaggtgc tcccgagccg
     3121 gacccgagcg gtctgcggtt ctaccagacc gagattggcg tgacgccgat caccaacttc
     3181 gaggtgacgt tccaggccat ggacgcgctg gcgatcacgg taggcgccac caacgcgttc
     3241 gacgagtatc ccgagaagcg caatgacatg cttcgcgaga tccagttcaa caacaacgac
     3301 aacagtgcgg ttgcgggtta tccgtcgttc tcgccgttcg gcatcaacgg cgcgtactac
     3361 tacggcaagc ttgtctactc gttctgagca agtcaggcag gcaaaaaaaa gagggcgggc
     3421 aacggtgccc gccctctctc gttttcgagt caaactactt gacgagctgg acttcgatct
     3481 ccaccagcat gcccggcgcc accaggcccg cgacctgcac cgtggagcgc gccggcagtt
     3541 tcggttgctc cttggtgccg aagaactgtg tgtaggcggc catgaaacct tcgaagtcca
     3601 tgcggccacc cttggcggga tcaccgacca ggtagacagt catcgccacg acgttgccga
     3661 agtcgagctt catggcgtcg agcgactcct tgatgcgggc gaaggtgctc aacgcctggg
     3721 tcttggtgtc gccccagtaa gccgccgtac cggcctccgc cttggggtcg agcggggcgg
     3781 gcgtggtgcc gctgtggaag atgatcgttt tgccggccgg cacctcgacc gcgcgcgcga
     3841 tcgggaaagt gctgccgccg ggcagcggat gacgcacgac gtcggccgcg tgcgccgcgc
     3901 cgatcgacgc gaatccgagc gcgcaggtga ccgcggcacg cacgtgactg gaacgatgtt
     3961 gtctggtcat ggagtctcct tagttagagc ggtgccgggc cgggaaaccg ggaaaacttt
     4021 ttctgggcga gtcgaagaaa agttttcccg gtttcccggc ccggcaccgc acgtttacag
     4081 cgtgttgatg taggccacga catctttgat ggccttttca tcgggaagtg tcgtaacgat
     4141 agcgcgcatc tgcgcgccgt aggtatcgcg ttcatcggtg ccgcgcagac ccttctgata
     4201 gttgacgagc tgcgcaacga gataccagtc cgagcgcgcc gccagcgcgg gcgagttcaa
     4261 acttgcattg ccttcgccct ttgcgccgtg acaactggcg cagttctcgt agagcttctt
     4321 gccatgcgcc acgtcacccg tgatcgtcgg cgcgggccgc ttcgaatcga gcttgccgat
     4381 gaactgcacg gcggcgtcga gtgtgccttc cgctttcagg cgcatgccca ccggtcccat
     4441 ctcgtgtccg ggcgcatctt cggcgggcgt gccgcgtgcg ccactggcga agttctcgag
     4501 ctggcgcgcg agataccagg gctccatacc cgaaagtttc ggtgcgcgga ttccgtagtt
     4561 gccgttggca ttggtgccgt ggcaaagcag gcagtagtcg aagtcattgc cggccgaggc
     4621 gctgccggcg atcagcgcca gcgcgatacc cgctgcgagt gcgttgcgcc gcttcatgct
     4681 gccaccgccc cggcggcgcg ttcccgcgcg tcgatattcg cgatagcgtg atgcgccgaa
     4741 gccatcgcgc cctcctgcca gcccgggtga taactcacct ggtctcccat gaggtagtgg
     4801 ttgcccaccg gcgtttgcag ggtcttgaac catttcttcc agagctcttc atcccattga
     4861 gccgcgcagc cgagcatgtg gttcatctgg tgccaggcga cgctcacgcc ggtctcgacg
     4921 taactgccat agccgggatg aattttctcg ccctgcgcga tcgcgagctt gaagcgttcc
     4981 gccggcggga gccgcgcaaa tttttcgccg gcgttcggat cgaacgtgta cgcacccagc
     5041 accacacctt tctgccggtt gatgccatgc gccggatacc acagctgctg gatgtcctgc
     5101 agcgtccatg aaatgccgcc gtagatgcct tccttctccc agaaccgctc cttcatctgg
     5161 aatccgatct tgaagagttt gccgcgcgga atggcggtga atcccgcgac gtattccttc
     5221 gggaaattgt tttcgattcc ggcgagcaga tgcatcggga tgcaattgag cacgtagtcg
     5281 gctttcgccc tgacgtactc gccgttgcgg cggtaggtga cctcgactcc cttggggagc
     5341 accttgatcg actgcacctg cgactcgagc tgcaccaggt gcccgatctt gcgcaggaaa
     5401 cccgccggaa tgcggtccat gccgccgacc ggctccatca tcatcgcggc ctgatcgtcc
     5461 gtttcgccga agttcatgat catcatgtat ctggatttca gcagctcacg cccatcgagc
     5521 ggcttcttga gcacatccgg attcgtgtaa tcgtgtaccg cgaggcccgc gttgctcgag
     5581 ccggcgtact tgaagctcgg gtcgaggttt cccaggaacc ggacgtattc gcgcaaacgt
     5641 tcgtaatcat ccttcgtcat cggcgccgca aactgttccg gcgtcatgga cttggacatg
     5701 agttccgcga cgaagccgcg cgagtccgtc atgtattcgc gcgcgcggac cggtttgccg
     5761 ccgaacatcg cgtcgtcctg cacccaggcc aggcggttgt cgttgatgaa tggcgcgagc
     5821 tcgacttcga gctccttgca atagttgagc agcgccttgt gatggcctgg aatgcgcgcc
     5881 gggccgcaat tgaaatagag atcgggatcc ttgtcgaaat tgcagacctg gggaaatccg
     5941 gtctcgtcga tgcgatcgcc gttgcgcaaa gtcatgttgc ggccgccggc gcggaatgac
     6001 gcttcgagca cctgcacgtc gtagcccttg cgcgacagct cgtaggctgc ggtgagtccc
     6061 gagattcccg cgccgagaat caggaccttc ttcggcttcc tgagagttcc cagttcggga
     6121 cgctccgcgt gtgcaatggc tggcagcaac ccaagggcca aggccgcgcg atataccgtc
     6181 gtcgatccgc cgaccgcgcc tgccatgttg agaaagccgc ggcgcgagag tttgttcgtc
     6241 atgttttgat tttccaccaa tcggtggttt taatttgttg cactgagcat acaagtaatg
     6301 tgccagcgcg aactggtcaa tgaaggcttt ctgatcggga gtgaagtttt tcttggcctt
     6361 gcggctgcac cgcagcgcgg cggtcgcggt gcggctgctt cagcgcaggg cagcatgatg
     6421 tagcaacccg gttaacgaaa accatgagac gaaacatcaa gacccaccgg acgcacgaag
     6481 caagttccga ttcccaatct ccgatttccc agcaagcggg atcgacgcac gacgttggcg
     6541 cgtcagcgcg tgaagaagga caggcttccg gcgcgaacat cgagggcggc gcagcgcgag
     6601 gcgatcagcg cgcaggtact ctttggtggt tctttcctac cgtgtgagat cagcgtttta
     6661 tctaattgag cgctgacagc tcaggcgcgc cgcctcgcgc aagcgaacgc gagtaataga
     6721 aatggtctcc atttctatta ctcgagcgcg cccgagagtg agcgtcggaa gcctgtcctt
     6781 cgtcacgcgc tgacgcgcca ccactgagcc ccgtcccctt ttgccagcaa atcaggaacg
     6841 gccccgaagc ggggcctgtc cccattcaaa gcacgtcgga gttcgatacg aacgcgatgt
     6901 gcgtgccgtc cggagaccat gacggcacgt tgatcgtgcc ctggcctccg tatacgtagc
     6961 cgatcacctt cggtggcgcg ctgccgtcgg tcggcatgat gcgcaggtag cagtgcttgt
     7021 agtacggatg atccgccgcc gcgacatcca gtccatacga aatgatgacg atccacttgc
     7081 cgtccggcga gatgtgcggg aaccagtcgt tgaacttgtc atcgcgggtc acgcgttcct
     7141 gggcggtgcc gtcgggcttc atgcgccaga tctgcatctg tcccgaacgc gtggagttga
     7201 agtagatcca ctggccgtcg ggcgaatact cggggccgtc atcgaggccg ggggagttcg
     7261 tgagattgat ctccttgccg ctgccgtcgg acgcgatctt gtggatatcc cactcgagca
     7321 ctttcgggtc cttcttcgtc gggcgcccgg cggtataggt cagccacttc ccgtcgggtg
     7381 accagccgtg caggtacgat ggtgtgagcg gggtgatgcg cttcggtatg ccgccgcgcg
     7441 tgggtatcgt gaagatggtc gattgcccgc ctccggtgct ctggtcgctg atcgcgagct
     7501 gcgtgccgtc gaacgacaac acgtggtcgt tgttgttgcg gatggcaaaa ccggtgtcga
     7561 tgagtgtggg cgtgcgcgtg gcgagatcga atttccacaa gcgcccgcgc gttgcgggat
     7621 cgttgccgct ggcgttgtag atcagggcgg agccgtcggg cagccagtta ggcgcttcga
     7681 aaggcacacg cgacgtgtag tacacattgc gatggccgct cgtgacgttc atcacctcga
     7741 gccggctgcc gatgtagtcg cggtagggct ggaagccgac cttgaccggc ttgatgacgc
     7801 gcacgtgtcg gaacactacg gtctctttca cgtccggatt gtgcgaggag aggaacaggc
     7861 cgacgtaggc gtcctcggag acgttgggca catccttgat ctcggccacc tcgaaaggtt
     7921 cgccaaattt tgcggcagag aaaatgaaca cattgccgcg gcgctcgagc tggatgacgt
     7981 tcgcaccctt cgcggccggc atggcggtct gcccggtgtc ttcgcccttt gccttgcgga
     8041 attgcagcga ggtgagcccg tctccatgca atgccccgtc gacgtaggcc gaatcgaaat
     8101 cggcgatcga ggtgcgcacc atgatgccgg ccttgcgatg cggatccacg cccttgccct
     8161 ggaactccac ctgcgcctgc acgatgaagt cgcccttcat gcgccggtag gcaaactgga
     8221 attcgtcgcg cttcgcccag atgttcacgc cgcctgcgct gagcgtgtac acctgcgatg
     8281 ccgcgttcca ggtggagtaa cccgcgatct tgggcgagcc cacgtccgcg tgcgcctcga
     8341 aatcgcccag cgggccgccg ttgggtgagg ccacgacaag tgccggcaat gccagcgtca
     8401 ctaacaacca tgcagttcgt ttcacagttc cccctcgttg aacgcccacc gattgttaca
     8461 gggaaatgag caacgtcccc atttcctacg acttaggtcg cagccaacag ggtgccgggt
     8521 ggggaaagtg gggagtgcca cactccccac tttccccacc cggcaccgtc tatctagcga
     8581 agcagggcgg tggcggtcca cagcatgggt gcctgtccat gcgtatcacc cgtcacgcgc
     8641 gggcgattca ggtagtgctg caggtcgttc ctcttgcccg tacccacgca cacctcacgc
     8701 agcaggcctt cgggagtgag gtagccgacg agcgcgatcc acgccttgcg cgccgcggga
     8761 ccgtaagtgt cttcggccag ccagccgttc ttcacgcccg tgacgaacgc atacgagaac
     8821 atggccgtgc tcgaggtttc ttcccagctt tccgggtgat cgatgagctg gcgccacatt
     8881 ccgttgtccg cctggtgggt gagcagcgtg gccatcatgc ggcgataggc cgagagcacc
     8941 gtcttgtacg atttgtgctt cggcggcaga tcgcgcagca gttcggccat gcccacggcg
     9001 acccagccat cgccgcgccc ccagtagaac ggcacgtccg gcgcgtggaa aaagagacca
     9061 tttggctgtt gcagcttggc gaggtacgcg accatcgagc gtgtggcgcg ctcgagatac
     9121 ctggagtcag ctgtggcgcg aaatgccgcg agctgcagcg cggtgatcat gtacatgtcg
     9181 tctatccacc agcgcgtctg gtaggaaaga ccatcgggca gcggccggtc ccattgcgcg
     9241 tcggcgaagg ccaggcccat gacgcgataa cgaaagcgcg cgtcctgcat gtacaactcg
     9301 agcggtagcg agccgaatac cgtgccgtcg acgtgatcgg cgcgcggcac gagcgttccg
     9361 ttggcggtca ggaacggatc gaaacgatcg acgaggcgct tcttcaggtc agcgtctttt
     9421 gtgaggttcg cgaactgcaa cgcgccggtc caggtggcca cgtgcgaata gtgcagcgcc
     9481 tgcgggccgt ggctgttcat ctccatgttt ggcgtgggga tgaatcgctc cgccacgcgc
     9541 ttgccgactt cggcgggcgc ggtgccggcc ggccagtcgg tgaaatactt gtcttgtgcg
     9601 ccagccgcgg acgcgaacac gaaggcaaag gcgatcatcg acgtcttgaa gaacttcatt
     9661 tgctggcact cacggagata acgaggggct tttgcgctcg cgcggcaaaa tccttcacat
     9721 aggtttccca ggcgacgcgg ccttcgaact gaccactctt cgtccatccc gcgccggtga
     9781 aatagcgcag cggtactccg tcgatggcct tgaccagcag caggttgtta gcgttgccct
     9841 tgccgcccgg cgcgtcctcg tgcgcaaagc ccgccggtgt cgcgtcgttc gcgaggatga
     9901 cggcaatgcc gagtccgccg tccttgttct cttcccagaa actcatccag cgcccgtcgg
     9961 gatccctggt gagcacggcc gccgggaaac ccgctgcctg tgggtgctcg ctgatgccga
    10021 tgccgaccat cgactcgttg gccgggaaat cgaaaacgct ttccactgca tcgaaattgc
    10081 ggccgcagtc gacggtgaag cgtttggtct cgacgacctg gccggcgggg ccgacgtccc
    10141 agggcgcgta gctgagtttg aatgccgctc gctggggtcc gttcgagagc acttgcgcgt
    10201 tcacgaagtt gtcggaggtc cacaatttcg cgccatccca gacgcccgtg ccgccggcgc
    10261 cgcgcgagcc gccgatgctg tacaggtcga ggccttcgcc ttcctcgtcc ttgtggaact
    10321 gatcgtgacc cttggcgtac cagcgatcga gaattggata gctgacgcgc ttggcccaga
    10381 cgtcgatgcc gctgccgcgc aggcgttcac cacccgcgcc cggtgaattg agcgccaggc
    10441 catacatgcg gtgcgcgatg cgatcgtttt cccaggccat gtcgtcgaag cgctcgggca
    10501 cgagacgcgc gtagacgcac ggcgtgagcg gcgccgaggc ggtggacaga ttttccagcg
    10561 tgaacgtggc gcgcttttca cccgccgcga aatcgtaggt gaacaccagc tcgtcatagg
    10621 acgcgccgcg atggtcgtgt tcgtagttgg tgatctgcga aggaatcacg cggcctttcg
    10681 agtcgcgcac cacgaggtgg tacatgcgcg cctcgggcat gacggcagcg acctgcgtga
    10741 atggaacgga gatgatttca cgcgggcggg ccgcgccgag gtcgtgcgtg acagcgatcg
    10801 tggcgccggc agcgaacacg ttcgaactca gcgcggccag ggccacgccc agagagatca
    10861 acttcatgtt tcccccaccg gccacggccg gaatgatttt cgctcggccg cgattgtcgc
    10921 ctattcgggg gcggttgggt tcggtgcgtg cctacggcac cacgatcgtc ggatcgacca
    10981 cagtccagaa cgcccattta ggatcgcggg ccgtatcgaa gagcagcgga cggttggtgc
    11041 gtgccaccgg ataggtgttg agccaggtgt gattgtccgc gatgccccag aagctcaccg
    11101 actgcacgct cgggcggttg aacgcgttga acaacgcgcg atacagcgtc gcctgctggg
    11161 agagcacgct ctgcggcggg ttcgcgccaa ggtcggcgag gcagggcgga atggtttgct
    11221 gcgagtaaca ggtgcccggg tcggcgtaga tggacacgtc gagctcggtc acgtgattga
    11281 ccagcgtgct cgacatggct tccaccgcgg tgaatgccgc cgtgacctgc gccacatcgg
    11341 cgcccagttg cagatggaac tggtgaccca cgccgtcgat ggggacgctg gcattgatca
    11401 ggtcctgcac gatgctcatc acgtttgcgc gcttgccagg aagctcggtg ttgtagtcat
    11461 tgagcatgag tttcatgccg gggttaccca gttgcgcgcg aacttcgccg gcaatccgga
    11521 acgcgtcaat cacgtattcg ccgccgggtc tgccggcgag cagataggcc tggtaccaca
    11581 cactgtcctg gcggtacggg ttggtggcgt tcggcgtgtc cgtggccgct tcgttgacga
    11641 cgtcccacgc atagacctcg ggataacgct gcatcaccgc tgtgatgtag gaccgcaaat
    11701 gctgctgcac ggaagcacgg tagctaggcg gattgttgag gtcaccaacg aacagccagg
    11761 ttggcgcgct ccgatgccag aggagcgtgt gcccgcgaag ccggatgccg ttggcaacgg
    11821 caaagtccgc gatgatgtcg gcctgcgtga agttcggagt cggtgccggc tgtgccgggg
    11881 agctgcccgg ctggttcggc cagatggaga ggggcttcat cgcgttttcg gccgtgatgc
    11941 tgctgaagtg cttgagcagc aacgcggagt ccgcgttcgt ggtggacgcg ggttcgatgg
    12001 ccgcgccgat cggaaaattg acgacgccgt actttgcggc gaggacggtc ttgagcgccg
    12061 gcgcgctgcc cgggctgggc agcccggtcg ggggaggtcc accaccaccg ggcggcggag
    12121 tgaatggttc gtcgccacca ccaccacagc cgccgagaag ggtggccgct accgccgcgc
    12181 cgaacagaac tttccgcatc attgaatcct ttcgtacctg gtgtggtggg ctcgatcctg
    12241 actcagttgc agtcgtcaat tcgtggaaaa gtgtcatcgc gcgcaagtcg tcgcgataga
    12301 ccttgagatc gacggcgcgc cagatgagta tctggcgagt ccagacatgg gagggggcga
    12361 tgttacagcg aatggccggg cggctggcat tgggaatggt tgtgctggct gcagcctgca
    12421 cgtcgaaggc cgaagagccg ttgatgagtg ggatattcgt cgaacacgcc gtgctccagc
    12481 gcgaccggcc catcgcggtg tttggccgcg cggcggcggg cgaagaagtc agcgtgacac
    12541 tggcgggtgc ttcagccagc gcgaaggccg atgcgaaggg cgcgtggtcg ctgacgctgc
    12601 ccgcgatgtc cgccggcggt ccgcacacgc tcacggcgcg cagcgcgtcg cgcacgcaga
    12661 ccgcgagcga cgttctcgtc ggcgacgtgt ggctgtgctc cggccagtcg aacatggagt
    12721 ggccggtgcg cgcgacgctc gatgccggct ccgaagtggc gctgtcggcc aatgaccgca
    12781 ttcgccaggt gaccattgcg cgggcgatca gtgcggcgcc gcggacggac ttcgaccagg
    12841 tgctcgaatg gaaagtggcg ggccccgcca ccacggaaca cttctccgcc acctgttact
    12901 acttcgtacg cgagctgcag aagaccgtga acgtgccgca gggaatcatt tcctcgaact
    12961 ggggtggctc gcgcatcgag ccgtggatga gcgagcaggc gctgcgcgga gtcgatggtt
    13021 accaggcgca actcgacttg ttaggccaat accgttccca gcggccgctg gcgttccagg
    13081 aatgggggga aaactggcag aagtggtggc tcgggcagca cgcggtgacc agaggtacgc
    13141 aaccctgggc agtgaagggc gactcgaccg cgtggcaacc cgcgccggcg gcgctgggct
    13201 attgggaaaa ctggggcgta cctgcgctcg gtcgttacga cggcctgatg tggtttcgcg
    13261 cgcatgtgaa gctgagcaaa gcgcaggcgc aagaagccga gcggctgtcg ttgggtcccg
    13321 tggacgacgt ggatgtgacc tgggtgaacg gccgcccggt cggcaacggc ttcggtgacg
    13381 aggcgcgcgt gtatgcgttg ccgccgagtg tgttgaaggc cggtgacaac cttgtcgtcg
    13441 tgaacgtgca cgacttctgg ggcaacggcg ggtttcatgg ttccgcggat caacgcgcgc
    13501 tgttgttggc cgacggcacg cgcgtgccgc tgacggactg ggagtacagc gtcgcgccgt
    13561 caggattatg gccgccgcat gcgccctggg agtcactctc cggcgtcagc gtgctgtaca
    13621 acgccatgat cgcgcctctc ggaaaatacg gcctgcgcgg cgtcgcctgg tatcagggtg
    13681 aggccaacgc cggcctcgac gatgcgcgca aataccaggc gctgctcgcg gcatggatgg
    13741 cggactggcg ccggcagttc gatgcgccgc tgccgtttct catcgtgcag ctcgccaatt
    13801 tcgggccgtt gtcaaagatg ccggtagata gcggctgggc gcaactgcgc gaggcacagc
    13861 gccgcgcggc ggtggcggat ggcaatgcgg cgatcgcggt aaccatcgac atcggcaatc
    13921 gcgatgacat ccatccggcg aacaagcagg acgtgggccg gcggctggca cgtgcggcgc
    13981 gccaggtcgt gtatggcgaa aaaatctcgg cgtacggtgc gcggcccagg tcggcgacgc
    14041 gcgctggcgg tgaggttgca gtcacgctgg atgattttga cggcaacctg ctggtggtcg
    14101 gggcgaaaga cccgaccggc ttcgagctgt gcggcgcggc gcaggagagt tgccgcttcg
    14161 tgcgcgcgca gcttgccggg ggcggcaaac tactgctggc cgatgccgcg gcggcaacgc
    14221 gagtgcgctt ctgctgggca gacagcccgt tgtgcaatct ctacgacagc acgggtctgc
    14281 cggtggggcc gtttgagatg gccatccagt aaatggggtc agaccccatt tacgcgagcg
    14341 agacgggccg ccctttcttg atcgactcgt agatagccat gaggaacctg acgtcggcga
    14401 gccccatctc acccgacacc ttggtcggcc gcttgttgat gatgcaatcg gcaaagtcat
    14461 cgagttcggt ggcgaactgg tcggcgaccg ggaaattgat ctcggtgccg tcgctgcgtt
    14521 ttccacgatt gccgttgtag aaatacgcgg ggtccatcgc gaaccagccg cgcgtggtgg
    14581 cggcgcggaa gccggcgagg ccgctggcag agtaggtgga gacgcagttc gcgaccacgc
    14641 cgcccggaaa cttcgactgc cagagaatgg tttcgtcgac gtccttgaac ttgacggtgt
    14701 cggtcttgat ctcggtggcg ttgacccaga tggggtcttc gccggtgagc atgcgcgtgg
    14761 tctgcaggca gtagatgccc acgtccatga gtgagccgcc gccggcgagc gcgcgattca
    14821 ggcgccactg cttcggatcg ccgatgggga atccaaagtt ggcgtcgatg atcttgacgt
    14881 cacccagcac tttttcacgc gccagccgga tgcactcgag atggttgggg tcgaattgca
    14941 ggcggtagcc cacgcccagc attttccccg ccttcttgca ggcgtcgagc atgcgctgcc
    15001 cgcgctccac cgaaatctcc agcggctttt cgcagaacac gtgtttgccg gcggcggccg
    15061 cggcgagcgt gtgctccatg tgcagcgcgt tcggcgtcac cacgtagacg atgtcgatat
    15121 ccgggttcga cgccatctga gccatgttct ggtagctgta gacagacttg tctgacaaac
    15181 cgtacttctt ctgccactcc accgccttcg atggcgtgcc cgtgacgatg cccgccaggc
    15241 ggcagttgcg tgtcttctgc agcgccggcg cgatctggtt cgtggacagg ctgccgagcc
    15301 cacaaagcgc aacgcctaac ttgcggcccg ccgcctgtgt gttgccaatg ctgctgagca
    15361 gcggcagggc ggcgagggcg gctgtccgtg taagaaattg gcggcgatcg gtttcggaca
    15421 tgactgatcc ttgcaaaggc ggggttcgga aaggcctgcg agtctagccg aacccacata
    15481 ccggtcgtat cgtcccaaag tgctgtcgca aggaaccgcg gactcacttg ccgttactca
    15541 accattgcgt gagtacgaca cacaagggac tgccgtgact attcaattca acgagttgct
    15601 accctccagg cggattcttg gtctgtcggt cgtgctggca ctggtcagct gcaccgcagg
    15661 cgatggaacg ggactcgacg caggtggtcg cccgattcct cccggaccac ctgccaacaa
    15721 cgacttccag cagatccagg acacgatatt cgggccgatc tgcagcggtt gccatcaggg
    15781 cgcgaatgcg ccgcaagggc tgcggctcga tgcgggcaat agttacgcgt tgctggtgaa
    15841 tgtggcgagc gccgaggtgc cgggcctcat gcgcgtcaac cccggcaatc cggacaccag
    15901 ctacctggtg cagaaaattc aaggcaacgc cgccgtcggc gctcgcatgc cggcgaacgg
    15961 tccgccgtat ctctcgcaga cgcagatcga tctcgtgcgc ggctggattg ccgcgggtgc
    16021 accgcagagc gcggcgcccg ccgatcagct ggtcgtcgtc agcagcattc ccgccgtctc
    16081 ggagcaggcg ggcgcgggtc tcggcaaact caccatcatc ttcaatggcg atgtggacag
    16141 ttcgctcgcg aacggcaacg cgttcgagtt gcgcgatgca aacgatcggc tcgtcaacgc
    16201 cgccgtgcgt gttccgtccg gacggccgaa cgtggtcgaa attacgacgc cgcagccgct
    16261 cgcggccggc agctatcagc tggcgatcct cggtgacgga ccctcgccac tcgccgatca
    16321 gtccggtcac gtgctcgacg gcgatgccga tggcaagccc ggtggcgata cgctcattcc
    16381 cttcgacgtc aaagccatcg ccaaagaagg atccgtccga tgatgcgcct aacgacttta
    16441 cgactcgcag gcgcgctcgc gctcgttgct ttcgccagcg cggcgcaggc ggaaccgtac
    16501 cttgcagcgc agatgggtct gaagtgcgtg cagtgtcacg tgaatcccac gggcggcggc
    16561 atgcgcacgg tgttcggcaa cgcgtttgcg caaacccagc tcgcggccaa acgcctgggc
    16621 gacgaggggg acatgtggac cggccaggtc atgaagttcc tgtcggtggg tggcaacgct
    16681 cgcgctaact acaacttcac ggatgttccg aaccaggaaa gctccaacga ctttgcagtc
    16741 gaggaggcgc gcgcctacct ggacttcggc gtgattccaa accgtttgtc ggtatacatc
    16801 gatcagcgtt tcgcgcccgg caattccacc aacctcgaag ccaacatccg ttactggatc
    16861 agcgagaaca gcgtgtacgt gaaggcgggg cgcatgtacc tgccgttcgg ctaccgcttc
    16921 gaagacgaca acgcgttcgt tcgccagctg tcgggcatca acatgcaggc gcccgacgaa
    16981 ggcgtcgaaa tcgggttcga gaagggatcg tggagcacgc agttcgcgat cagcaatgga
    17041 accgccggcg ctcctgaagt cgacagcggc aaacagctca ccgcacgcac ggagtacgtc
    17101 ggctcggcgt ggcgcgcagg ggtgagcgcg gtggccaacg acacggatgc gggcgatcgc
    17161 atgggcggca gtgtcttcgg cgcctatcgt ctgggtccgg tcaccttgct tggcgaggtc
    17221 gactacttcg atgacgacag catcggagtc gaaggccgga agttgatggc ttcactcgtc
    17281 gaggccgact ggaagcttcg gcagggccac aacctcaagc tcacctacga atggctcgag
    17341 cccgatgacg atgtggacga ggacgagcag acgcgcacca gcctggcgta cgaatggtcg
    17401 cctatccagt tcgtccagtt gcgcggtgga gtccggtttt acgacggcat cccgcagagc
    17461 gacctgcaaa accgcacaca ggcattcctg cagctccatg ggttcttcta gccggctagc
    17521 taggggagat gacgattctt ccctcgttct ttgcaacgac gtaggggttt cgcccggcaa
    17581 cgatctggaa gaacgctttc acgctgagct tcagcaggga tacgtcgagg tggcgaagct
    17641 gcagcagcga caggccgatg attgaccagc acaaccagag agtcgtgatc gtggcgttcg
    17701 agaagcgggc cctgacaaag tagtgcaacg ccaaaatgcg gcgatagtag gcgacctttc
    17761 ggttgaccct gttgtcctgc tcgcgatgat gcagggcgta ggtgctgtcg atgacgtcga
    17821 tgccgtttgg aagtcccagt gccacgccgt agttatcgcc gatgcccccg gggtcgagca
    17881 ccgggtcgta gggcgacgac aggagaaact ctctccgtac gaggtctgcg ccaagagaat
    17941 agaacctggt ggtcaggaaa ggtcgctgcc attgtgtaat gagaggccac cccgcaaggc
    18001 tctccctgtt acctcttttc cgataccact ccttcatcca ggcgaggaca ggccgtgtaa
    18061 atcgattcgt tttcacgccg tcgatgggcc cccagaccga gagctgaaat acgaagtgat
    18121 agagcgcggt ggagaatctc cggggtgggt attggtcgac ccagactccg ctttcgagct
    18181 gccgcagtct tcccgcgaca gcgccgcatc cgggatgttt cgcaacatgg tttgccagat
    18241 gctgaagatg gtccctgggc aattcgatgt cgtcgtcgca caggaatatc cagtcgcttt
    18301 tcgcagcttc gattcccttg tttctctgga tgcataccga gggcgccgac gagagcaata
    18361 tcaacggcag cgtatgaaac tcacttgcca gcgcttttgc gtcatggaag ccctcatcgc
    18421 tggcatcgac gacgatgact tcggcgggtg gcgacgtctg ttgggccaac gacgagagcg
    18481 cgttgcgaac ccgtccggga cgattcctgg taggaatgac cacactgatg tccactacgc
    18541 atttcctcgt gctacgcggc tgtacccgtg gcggatagta ctttcaacac ccgggcgagt
    18601 ttcagcaaac actcgcgggc cgcggggaac cgcctcgagt tgtcgcggta ctcatcggga
    18661 tggctggcgc gcccgaggtc ggcacgcagc tcaccacgcg attgctgcga cgtcgacgca
    18721 ttgcgtgggc cctgggcgga gggctaactt ccgtccacag ggagcgagac gcaatggata
    18781 agcctgtgca gtcgcaaccg gccgctttcg attccatggc caagggttac gacgcggaat
    18841 tcaccgcgtc ggttctcggt cgtgtcctgc gccccatggt ctgggagagg ttcgcagcct
    18901 gtttcgcgcc cgggtcgtcg ttgctcgagc tcggctgtgg cacgggcgaa gacgccatcc
    18961 acctggcgaa gtcgggtcac cgcgtattgg ccaccgatgc ttcggctcaa atggtcgact
    19021 gcgcgcgccg caaggctgag gaggccggtg tcgccgatcg catccggttt caggtcgtgc
    19081 ccatggagtc gctgtccgcg gtgctcggta ccgaagtctt cgacggtgtg tattcaaatt
    19141 tcggcgccgt gaactgtgcg ccggacctcg cggcgctcgc aggcgtattg gcgccgcggc
    19201 tcgcgccgaa tgcgccgctc gtctgggtga tcatgggccg cttcgtcccc tgggagatgg
    19261 cttggtatct gttgcgcggt gaccgtcgtc gcgcgtttcg caggtttcac cgcggcggaa
    19321 gcgagtggcg cggtctgact atccactacc ccacgcctgg caattgcgcg cgccgcctcg
    19381 agccgcattt tgttgtcaac gtggcgcggc cgctcggcgt gatattgccg ccgagttacg
    19441 ccgcgggctg gctcgagcgg actccacgaa tgcgtgcgtg gctgtcgcgc ctggagcgca
    19501 cattgttcga gcgggcgctg ggagcgggct tgagcgatca ctacgtgctc gaagcacgcc
    19561 gcgctgcatg aagacattcc gcgcattctg gacattgctg ccgccgcaac tgcggcgccg
    19621 ctgtcttgcg cttttcggtc tggcgctcgc catggcggcc accacagtcg ccgggctcgt
    19681 gtccgtgatt ccgttcttcc aactgctttc tgattccgag gccaccccca cgacgcgctg
    19741 gctgcatggc attcgcgcca cgctcggcat tgccgatgag cgggtgttcc tcgccgccac
    19801 gggtgtggtc ttcgtgctca cgctcatcgt cgccaacgtc atcaactact tcggtacgcg
    19861 cagcattcac tccttcgcgc tgggcgctgc cgacgccatc cgcgtgcggc tattcggcgt
    19921 ctacttgcgc agcgactatc tcttccacac ccgtcacgat ggtgcgcgcc tcggcaacga
    19981 cgtgttgcac gagtcagacc gtatcattgt gctggcagcc gtcttctttc aggccgtcac
    20041 ggcgacgggc gtggtggtgc tcatcgcggt gtcgctgatc atcatgaatc cctgggtgtt
    20101 gctcgggaca ttgctcgtgt tcggttcagc ctacgcgctc atcttcgcgc ggtcgcgcag
    20161 acagctccgg gtgaacagta tcgaactcac ccgcgagtcg gcgcgccgcg ccatggtagt
    20221 gaggcaggcg ttcgcggcca tcaaggaact gctcgtgatg cgactcgatg cgccgttcca
    20281 gcgccagttt ggtgaggcca cggcccgggt gtcgcggtcg ctggcccata cgaacgccat
    20341 cggcctgcga ccgaagtatg ttctcgaatg cctcgcggcc ttcgggctcg tcaccgtcgc
    20401 gctcgtgctg cacgtccagg agggtgcccc gtggctgccg caactcacct tcctggcgct
    20461 ttgcgcctat cggctgttgc cgagtctgca gcagctcttt gtgggcctcg tggccgtgcg
    20521 ggcgcaccgc gacgtgctgt tcagaatgtt gaagagtgtg gaagaggaac ctgcatcggg
    20581 cggtgcggtt tccgtgccga agctcgaggg cgcgccagca cgcgacatcg tgttcgaaga
    20641 cgtcaccttt cgctacggag ccgaggcgcc cgccgtgttc gagcatctga atctgcgtat
    20701 ccgcgcgggc gaggccatcg gtgtaactgg gcagaacggc tcgggaaagt cctcattcgc
    20761 cgatctgctg ctcggc
//
LOCUS       DASQJS010000146         8208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2589196, whole genome shotgun sequence.
ACCESSION   DASQJS010000146 DASQJS010000000
VERSION     DASQJS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2589196"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..897
                     /locus_tag="VEW08_06020"
     CDS             58..897
                     /locus_tag="VEW08_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HYJ40327.1"
                     /translation="MELRFRILNVFTSGSRLSGNPLCVVEDGSGLEPAAMQALARQFN
                     VSETTFILPSKRAAALVRIFTPAYEMPFAGHPTLGTAHVCRALGLGGDQLGLEMRAGI
                     IPVTANGDHWTLRAAAATSRLVEVQPPELARLLGLEPSDIGFQPLWMKAGREQLVIPL
                     TSEAAVRRVRPKAELFERLKSVDGAGQAYVFASVGAKKILSRFFFPDNGAILEDPATG
                     SACANLGAWFVAMGRRPPLNFEVSQGEFIGRPSTLQLEVNSDNEVFVGGEVIEIGSGI
                     VNL"
     gene            907..2208
                     /locus_tag="VEW08_06025"
     CDS             907..2208
                     /locus_tag="VEW08_06025"
                     /inference="COORDINATES: protein motif:HMM:NF005455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HYJ40328.1"
                     /translation="MSADSYHKRKLGSRVLQPETQMMGYGYDPKLSEGSLKPPIFLTS
                     TFVFRNAQDGKDFFDYTSGRRQPPPGQAAGLVYSRFNNPNLEVLEDRLGLWEGGEAAC
                     VFSSGMSAISTTLWAYLKPNDMLLMSEPLYGGTETLIDKTMPAFGIGALRVADAINRE
                     AVMGGAKAAVEKAKARGGKVGLLVTETPANPTNSLVDLQLWREASEWIAGQQGGARPP
                     VAVDNTFLGPVFQKPLEHGADLVMYSLTKYVGGHSDLVAGGVIGPKAMVAPVRALRSS
                     LGTQLDPNTSWMIMRSMETLALRMRASADNAGRVAGYLAQHPKVASVNYLGHLKSGDP
                     RAEVFARQCLSAGSTFAFSIRGGEAEAFRMLDALQVMKLAVSLGGTETLISHPASTTH
                     SGVPKETRDRMGVTDALIRISVGIENADDLIADLEQALSKI"
     gene            2312..3805
                     /locus_tag="VEW08_06030"
     CDS             2312..3805
                     /locus_tag="VEW08_06030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ40329.1"
                     /translation="MLSKPQLASWHIFSICLGAFGIQFGFALPQANATRIFQNLGASL
                     DDVPLYFLAGPITGLIVQPLVGYYSDRTWTRFGRRRPYFLAGAALAAVTLLAMPNATT
                     LWVAVLMLWVLDASLNFTMGPFRAFVADQMSTEQRATGYLMYMFFASVGAVIGSLLPW
                     MFTSLGVSSHAPPGEISLAVKYAFGVGATLMVLAVCWSAFTTREYPPEMLEKFDSAEL
                     ERDSDRSPAKMRRHAFVWLALGSTGLLFAWLSGVRPALYVLVCASLAYGAFLLVASRM
                     KSNNAFTTILGELDSMSASMRWLALVQFCSWFTLFAVFVYTTPAVAMMQFSGSVPGSE
                     GYEAGANWVGVLFATYNGLAAVAAFFIPYFVRRSGLRKVHRVNLWIGTVGLLSMMLIR
                     EPEWLLLSMVGLGVAWASIIALPYAMLANSLPSRKMGVNIGIFNIFIVIPQLLAASVL
                     PLLLDVFAGGDASYALVIGAIGWFLAGVAVLHVKDNTSLADRAEAHR"
     gene            complement(3773..5791)
                     /locus_tag="VEW08_06035"
     CDS             complement(3773..5791)
                     /locus_tag="VEW08_06035"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HYJ40330.1"
                     /translation="MFTALAAQASAAAQARDGTCVDSPQKVLTVCVAVDEGGPYYDVN
                     RGDREIIAHARLGLVLEGFGNRPATHVANARRAATDQRWEQPWGEQRYIQDRHNELRV
                     TLSGADRALTEPYDLAVRVFDDGFGFRYEFNQIAATRDVAIVDELTEFRLAGAFDAWW
                     YPARHADRDEYLYHRGPLYEVNLAETPLTLQSPGLYLSIHEAALVDYASMNLRRTAER
                     TLKADLMPWSDGVLVRRRGSFTTPWRTMLVGETPMALADSRMELNLNEPNGIADTSWI
                     HTGKYVGIWWEMHLNRSTWASGEKHGANNANVKRYIDFAAKNGFGGVLVEGWNRGWDG
                     DWIANGKHFSFTESYPDFDLPQLSAYARERGVRLIGHNETAGAIENYERQLDAAMALY
                     AANGVNVVKTGYVKPSGSIERTLADGTIGHEWFAGQYRVDHEVKVAQVAATHRISIDA
                     HEPVKDTGLRRTWPNMLSREGARGQEFNAWGRPTNPPEHLTILPFTRLLAGPMDFTPG
                     IFDLTFGKTELKERVQSTLATQLALYVLIHSPVHMAADLPENYEKRPDAFQFIRDVPT
                     DWETSRTLQGEIGDFVVIARQQRGAADWYLGATTDENARRLDIPLDFLERGRRYEAQI
                     YRDAADADFRTNPAALVIEKRTVTAKDHIAAALAPGGGLAVRFRAIGK"
     gene            complement(5884..6906)
                     /locus_tag="VEW08_06040"
     CDS             complement(5884..6906)
                     /locus_tag="VEW08_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYJ40331.1"
                     /translation="MNLSKDEVGGPVTLDKLAKLAKVSVSTVSRALNDHPSISSPMKK
                     RLWALARKHGYVFRRHMPASPIGAEGAITIVTPRVRGRPLPLSHPFFLELLASIGEAA
                     RDRGCDFTVSHLTPANYEDLVHATTTGRANGVIFLGQGILHDEFNRLAQTDSRFVVWG
                     AQFPGQRYCTVGTDNMLGGKRATQHLLKLGRKRIVFLGGSDPEATQRHRGYTEALTEA
                     KVKIDDALIGRVEFEEESAEAAIDALQMRKIPFDGVVAASDLIALGAIRALSRAGRKV
                     PRDVSVVGYDNMLLSRLSTPTLSTVQQDIPKAGRLLVAKLLDWTGDVRPEMLSTELII
                     RESCGA"
     gene            7191..>8208
                     /locus_tag="VEW08_06045"
     CDS             7191..>8208
                     /locus_tag="VEW08_06045"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYJ40332.1"
                     /translation="MKPIQNFTSRALFTIVGGTVLINPAFAQDQPAEAVPEVVVTGLR
                     ASLESAQGIKQNSDTFVDSITATDIGAFPDKSVAEALQRVPGITVSRLQSSDDSNHFS
                     AEPATVLIRGLTFVRTEFNGRDSFSADGYRGLNFNDVSPELMQGVDTYKNQTAEMIEG
                     GIAGVVNLRTRTPFDSDGQTFALTGRVNYGTLSEEPTYEASGVYSNTWETDSGRFGLL
                     ANAAYSNIVTRTEAVNMTRIATFCSDYPQVGGAFPNANLDADGNVVCNNNPYGGTGWR
                     YAPGQVNFSQVDYDRTRTGAALTLQYENDAKNLLLTLTGVHSKYENPWIERSANISWP
                     TGAGF"
BASE COUNT         1416 a         2587 c         2598 g         1607 t
ORIGIN      
        1 attacgaggg tttctctcac gctacttggc cgaagggttg tgaaataatc ctgcgccatg
       61 gagcttcgct ttcgcattct caatgtcttc acgtcaggca gccgcctctc cggaaacccg
      121 ctgtgcgtgg tcgaagacgg ctcggggctg gaacccgccg ccatgcaggc gctggcgcgc
      181 cagttcaacg tgtcggaaac cacgttcatc ctgccttcga agcgggccgc ggcgctggtg
      241 cgcatcttca cgccggccta cgaaatgccg tttgccggtc accccacgct tggaaccgct
      301 cacgtctgtc gcgcgctcgg gctgggcggc gaccagctag ggctcgaaat gcgcgccggc
      361 atcatcccgg tcaccgcaaa tggagatcac tggacactgc gcgccgcggc agcaacttca
      421 cggctggtcg aagtccagcc gccggagctg gcgcgcttgt tagggctcga gccgtccgac
      481 atcggcttcc agccgctgtg gatgaaggcg ggtcgcgagc agctggtcat tccgctcacc
      541 agcgaagcgg cggtgcggcg ggttcggccg aaggccgagt tgttcgagcg gctcaagagc
      601 gtcgatggcg cgggccaggc gtacgtgttc gcctcggtgg gcgcgaagaa gatcctcagc
      661 cgctttttct ttcccgacaa cggcgcgatt ctcgaagatc cggcgaccgg ttctgcctgc
      721 gccaatctcg gcgcctggtt cgtggccatg ggtcgccggc cgccgctcaa cttcgaagtg
      781 agccagggtg aattcatcgg ccgtccgtcc actttgcaac tggaagtgaa ttccgacaac
      841 gaagtattcg ttggaggtga ggttatcgag ataggatcgg gcatcgtaaa tttgtaagga
      901 gcgctcatga gcgccgatag ctaccacaag cgcaaactcg gcagtcgagt gttgcagccc
      961 gaaacccaga tgatgggtta cggttacgat ccgaaacttt ccgaaggatc gctgaaaccg
     1021 ccgattttcc tcacgtccac gttcgtcttc cgcaatgcgc aggacggcaa ggatttcttc
     1081 gactacacct ctggccggcg ccagccgcca ccgggtcagg ccgcgggcct ggtgtactcg
     1141 cgcttcaaca atccgaatct cgaagtgctc gaagaccggc tgggcttgtg ggaaggcggt
     1201 gaggctgctt gcgtcttttc gagtggaatg tcggcgattt ccaccacgct ttgggcctac
     1261 ctcaagccga atgacatgct gctcatgagc gagccgctgt acggcggcac cgaaaccctc
     1321 atcgacaaga ccatgcccgc attcggcatc ggcgcactgc gcgtggccga tgccatcaat
     1381 cgcgaggccg tcatgggcgg cgcgaaagcg gcagtggaga aggcgaaggc caggggcggc
     1441 aaggtcgggt tgttagtcac cgaaaccccg gcgaacccca ccaacagcct ggtggacctg
     1501 caactctggc gcgaggcatc ggaatggatt gccgggcagc agggcggtgc gcgcccaccg
     1561 gtcgccgtgg acaacacttt cctcggcccc gtattccaga agccgctgga acatggcgcc
     1621 gatctggtca tgtattcact gacgaaatac gtcggcggcc attcggacct ggtggctggc
     1681 ggtgtcatcg ggcccaaggc catggttgcg ccagtacgtg cgctgcgcag tagtttggga
     1741 acccagctcg atccaaacac ctcgtggatg atcatgcgct ccatggaaac gctggcgctg
     1801 cgcatgcgcg cgtcggccga caacgccggg cgcgtcgccg gatacctggc gcagcatccc
     1861 aaggtcgcga gcgtcaacta cctggggcac ctcaagagtg gcgatccgcg cgcggaggtg
     1921 ttcgcacggc aatgcctgag tgccggttcc acgttcgcgt tttcgattcg cggcggagaa
     1981 gccgaagcct tccgcatgct ggatgcgctg caggtgatga aactcgcggt gagcctcggt
     2041 ggcaccgaga cactgatttc acacccggca tccaccacgc attcgggagt gcccaaggaa
     2101 acccgcgacc gcatgggcgt caccgacgca ttgatacgta tttccgtggg tatcgagaac
     2161 gccgacgacc tgatcgccga cctcgagcag gccctctcca aaatatgacg gacgccgccg
     2221 cttcggcggc gttttccgat gtggggcgca gcccctgaca agtcgcagcc cctgacaagt
     2281 cgcagcccct ggcaatgcag cccctgggaa tatgctttcg aaaccccaac ttgcctcatg
     2341 gcatattttc agcatctgcc tgggggcttt cggcattcag ttcggtttcg cgctgccgca
     2401 ggccaatgcc acgcgaattt tccagaatct cggcgcttcg ctcgatgacg taccgctgta
     2461 tttcctggcg gggccaatca cgggcctgat cgttcagccg ctcgtcggct actactcgga
     2521 tcgcacgtgg acgcgatttg gccggcgccg cccgtacttt ctcgctggcg cggcgcttgc
     2581 ggcggtcacg ctcctcgcca tgccgaatgc aacgacgctg tgggtcgcgg tgctcatgct
     2641 gtgggtgctc gacgcttcgc tcaatttcac gatgggtccg ttccgcgcct tcgtcgccga
     2701 ccagatgtcg acagagcagc gggccaccgg ctatctcatg tacatgttct ttgcgagcgt
     2761 gggcgccgtc ataggcagtc tgctgccatg gatgttcacg agccttggcg tctcgtcaca
     2821 cgcgccaccg ggcgaaatca gcctggcggt gaagtacgca ttcggggtgg gagccacttt
     2881 gatggtcctc gcggtgtgct ggtccgcctt cacgacgcgc gagtatccgc ccgagatgct
     2941 cgagaagttc gatagcgccg aactcgaacg cgattcggac cgctcgcctg ccaagatgcg
     3001 ccgccacgcc ttcgtctggc tcgcgctcgg atcgacaggg ctgctcttcg cctggctgtc
     3061 cggcgtgcgc cccgcgctgt atgtgctggt gtgcgcttca ctcgcctacg gcgcattcct
     3121 gctggttgcc agccggatga aaagcaacaa cgcattcacc accattctcg gcgagctgga
     3181 ttcgatgtcc gcctccatgc gctggctcgc cctggtgcag ttctgctcct ggttcacgtt
     3241 gtttgcggtc ttcgtgtaca cgacgcccgc cgtggcgatg atgcaattca gcggcagcgt
     3301 tccgggctcg gaaggttatg aggccggcgc caactgggtg ggcgtcctgt tcgccaccta
     3361 caacggcctc gccgccgtcg ccgcgttctt cattccgtat ttcgtgaggc gatccggcct
     3421 gcgcaaggtt caccgcgtca atctgtggat cggcaccgtg ggcctgctgt cgatgatgct
     3481 gatccgcgag cccgagtggc ttctgctgtc gatggtcggt ctcggggttg cctgggcctc
     3541 catcattgcc ttgccctacg cgatgctcgc caacagcctt ccgtcgagga agatgggcgt
     3601 gaacatcggc atcttcaata tcttcatcgt gatcccgcaa ctgctcgccg cgagcgtgct
     3661 gccgctgcta ctcgatgtgt tcgccggcgg agacgcttcc tatgcgctgg tcatcggcgc
     3721 catcggatgg ttcctcgccg gcgtcgccgt gcttcacgtc aaagacaaca cgtcacttgc
     3781 cgatcgcgcg gaagcgcacc gctaatccgc cacctggcgc cagcgccgcc gcgatgtggt
     3841 ctttcgccgt cacggttcgc ttttcgatga ccagcgcggc ggggttcgtc cggaaatccg
     3901 cgtcggccgc gtcgcgatag atctgtgctt catagcgccg gccgcgttcg agaaagtcga
     3961 gcggaatgtc gagcctgcgc gcgttttcgt cggtggtggc acccaggtac cagtcggccg
     4021 cgccgcgctg ctggcgcgcg atcaccacga aatcgccgat ctcaccttgc agagtgcgcg
     4081 aagtttccca gtcagtaggc acgtcacgga tgaactgaaa ggcatcgggg cgtttttcat
     4141 agttttcggg caggtcggcc gccatgtgca ccgggctgtg gatgagcaca tagagcgcga
     4201 gttgcgtcgc gagcgtcgat tgcacgcgct ctttcagttc tgtcttgccg aaggtgaggt
     4261 cgaagatccc cggcgtgaaa tccatggggc cggcgagcag tcgcgtaaac ggcaggatgg
     4321 tcagatgctc gggcggattc gtggggcggc cccacgcatt gaattcctgc ccgcgggcac
     4381 cttcgcggct cagcatgtta ggccaggtgc gtcgcaggcc ggtgtccttc acgggctcgt
     4441 gcgcgtcgat ggagatgcgg tgcgtggcgg cgacctgggc caccttcact tcgtggtcga
     4501 cacgatactg gcccgcgaac cattcgtggc caatggtgcc gtcggcgagc gtgcgttcga
     4561 tggagccact cggtttcacg taaccggtct tgaccacgtt gacgccgttc gctgcgtaca
     4621 gcgccatggc cgcgtcgagc tgacgctcgt agttctcgat cgcaccggcg gtttcgttgt
     4681 ggccgatcag ccgcacgcca cgctcacgtg cgtaggccga gagctgcggc agatcgaaat
     4741 ccgggtacga ctcggtgaag ctgaagtgtt tgccgttggc gatccagtca ccgtcccagc
     4801 cccggttcca gccttcgacc agtacgccgc cgaagccatt tttcgcggcg aaatcgatgt
     4861 agcgtttcac gttggcattg ttggcgccgt gtttctcgcc gctggcccag gtcgaacggt
     4921 tgaggtgcat ttcccaccag ataccgacgt atttgccagt gtggatccac gaggtgtcgg
     4981 cgataccgtt cggctcattg aggttcagtt ccatgcgcga gtcggcaaga gccatcggag
     5041 tttcaccgac gagcatcgtg cgccatggtg tcgtgaaaga tccccgcctg cgcaccagca
     5101 cgccatcgga ccacggcatg agatcggcct tgagcgtgcg ctcggcggtg cgtcgcaggt
     5161 tcatgctggc gtagtccacg agcgccgctt catggatcga tagatagagt cccggggact
     5221 gcagtgtcag cggagtctcc gcgaggttca cctcgtacag cggtccgcgg tggtagagat
     5281 attcgtcgcg gtcggcatgg cgcgccgggt accaccacgc gtcgaaggcg cctgccagcc
     5341 tgaactcggt gagctcgtcg acgatggcaa cgtcgcgcgt tgcagcgatc tggttgaact
     5401 cgtagcgaaa gccgaagccg tcgtcgaaca cgcggacggc gaggtcgtag ggctcggtga
     5461 gcgcgcggtc ggcgcccgac agcgtcacgc gaagctcgtt gtggcggtcc tggatgtatc
     5521 gctgctcgcc ccagggttgt tcccagcgct ggtcggtggc cgcgcggcgc gcgttggcca
     5581 cgtgggtggc cggccgattg ccaaagcctt cgagcaccag gcccaggcgt gcatgcgcga
     5641 tgatctcgcg gtcgccgcga ttcacatcgt agtagggacc cccctcatcg acggccacgc
     5701 acaccgtcag cactttctgt ggcgagtcca cgcaggtgcc atcgcgtgct tgcgccgcgg
     5761 ccgaggcctg cgcggccagg gcagtgaaca cgaggccaca agcagcagcc gaagccatcc
     5821 tcataaacgg tttcctcgag actaactatg gttgttcatg acgcgtgcct gaacgattgc
     5881 aggtcaggcg ccgcaggatt cgcgaatgat caactccgtc gacaacattt ccggacggac
     5941 gtcgccagtc cagtccagga gcttggcgac cagcaggcgg ccggctttcg ggatgtcctg
     6001 ctgcacggtg ctcagcgtgg gcgtgctcag ccggctgagc agcatgttgt cgtaaccgac
     6061 caccgatacg tcgcgcggca ccttgcgccc cgcgcggctc aaggcgcgga tggcgccgag
     6121 cgcgatgagg tcgctggcgg caacgacgcc atcgaacggg atcttgcgca tctggagcgc
     6181 gtcgatcgcc gcttcggccg attcctcttc gaactccacg cggccgatca gcgcgtcgtc
     6241 gatcttcacc tttgcctcgg tcaacgcttc ggtgtagccg cgatgacgct gcgtcgcctc
     6301 cgggtcgctt cccccgagga acacgattcg ctttcgccca agcttcagca gatgctgggt
     6361 ggcgcgtttt ccgcccagca tgttgtcggt gcccaccgtg cagtagcgct ggcccgggaa
     6421 ctgcgcgccc cagacgacga agcggctgtc ggtctgcgcc agccggttga actcgtcgtg
     6481 cagtattccc tggcccagga atatgacccc gttggcgcgg cccgtcgtgg tcgcgtgtac
     6541 caggtcctcg tagttagccg gcgtcaggtg gctcacggtg aaatcacagc cgcgatcacg
     6601 cgcggcttca ccgatgctgg cgagcagttc caggaagaag gggtgggata gtggcaatgg
     6661 acgtccgcgc acgcgcggcg tcacgatggt gattgcgcct tcggcgccaa tgggactcgc
     6721 cggcatgtgg cggcggaata cgtagccgtg cttgcgtgcc agcgcccaca gccgcttttt
     6781 catggggctg ctgatcgagg gatggtcgtt gagcgcacgg gagaccgtgg agaccgaaac
     6841 cttcgcgagc ttcgccagct tgtcgagggt gaccgggccg cccacttcat ccttcgaaag
     6901 attcataacg cctcaaatcc tttcctaaac cgaccttacg cggtgcagca ggtgtacccg
     6961 tttacgcaaa tttacgcaaa aacccttggg actattgcgc aatttttcgc aacttgggta
     7021 gtgtacacgc gtcgtatcgc tgaaatcgcg gaattagcga acgcaattat acgcaaaacg
     7081 atttcaaggg ggctcatgaa aactcgcaac tgtggtctaa cccaatggct ttgtgctgcg
     7141 cgcgccgtgt tcgcgctgcc gacaaacaac cagtcagtgg aggtggcgtc atgaagccga
     7201 tccagaattt cacgtcgcgg gcgctgttca ccatcgtggg cggcacagtc ctgatcaatc
     7261 ctgcgttcgc acaggatcag ccggcggagg cggtgcccga ggtcgtcgtc acgggcctgc
     7321 gcgcatcgct cgaatcggcg cagggcatca agcagaactc cgacaccttc gtcgattcga
     7381 tcacggccac cgacatcggc gcattcccgg acaagtcggt cgccgaagcg ctgcagcgcg
     7441 ttcctggcat caccgtctcg cggttgcagt cgagcgatga cagcaaccac ttctcggcgg
     7501 aaccggccac ggtgctcatc cgcggcctga cgttcgtgcg cacggagttc aatggccgcg
     7561 acagtttctc ggccgacggc tatcgcggtc tcaacttcaa cgatgtgtcg cccgagctca
     7621 tgcaaggtgt ggacacctac aagaaccaga ccgcggagat gatcgaaggc ggcatcgccg
     7681 gcgtagtgaa cctgcgcacg cgcacgccgt tcgattcgga cggccagact ttcgcgctga
     7741 ccggacgcgt caactacggc acgctttccg aggagccgac ctacgaggcg tcaggtgtgt
     7801 acagcaacac ctgggagacc gacagcggcc gattcggttt gctggccaac gccgcgtact
     7861 cgaacatcgt gacgcgcacc gaagcggtca acatgacgcg catcgccacg ttctgctctg
     7921 attacccgca ggttggcggt gcattcccga atgccaatct cgatgccgac ggcaatgtgg
     7981 tgtgcaacaa caatccgtac ggcggcacgg gctggcgcta tgcgccgggc caggtgaact
     8041 tctcgcaggt ggactacgac cgcacgcgca ccggcgcagc gctcacgctg cagtacgaga
     8101 acgacgcgaa gaacctcctg ctgacgctga ccggcgttca ctcgaaatac gagaatccgt
     8161 ggatcgagcg cagcgccaac atcagctggc cgaccggcgc tggattcg
//
LOCUS       DASQJS010000147         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1965576, whole genome shotgun sequence.
ACCESSION   DASQJS010000147 DASQJS010000000
VERSION     DASQJS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965576"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1257)
                     /locus_tag="VEW08_06050"
     CDS             complement(214..1257)
                     /locus_tag="VEW08_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HYJ40333.1"
                     /translation="MKFLRQLAAALFALQFSLAAIADGSATATRAAFLRVIDRPRVAL
                     KPELAEMAPVEGLRKYHLWFSSDATERVPGYLVLPDGESFKGKRPVVIVLHGTGGTKE
                     QVAATVLKAVQAGFIGVAIDGRFHGERTKAGTGAAEYNLAIAQAFRTGEGHPFYYDTT
                     WDVMRLIDYLGTRKDVDFARIGLTGFSKGGIEAYLTAAADPRIAAVVPYIGVQSFKWA
                     LENGQWAARIATIQDAFNAAAAAAGKPARSVDFVREFYARVVPGIDGQFDGPAMLAAI
                     APRPLLVINGDSDANTPVAGVRLSVAAAKPIYESANAGDKLQLIIQENTPHRVNPESI
                     DAGIAWFVRWLKP"
     gene            complement(1292..2017)
                     /locus_tag="VEW08_06055"
     CDS             complement(1292..2017)
                     /locus_tag="VEW08_06055"
                     /inference="COORDINATES: protein motif:HMM:NF028481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYJ40334.1"
                     /translation="MITLYTFGPLFGLPDASPFVMKGEMLLKLAKLEYQTSNKGFMRA
                     PKGKLPYIDDNGTIVADSTLIRLYLEEKYGVDFDRNLSAREKGIAWATEKMLEDHLYW
                     VLVYWRWMKDANFEKGPANFFRRAPAFIRPLVIWKVRGNVRRSLHAHGIGRHQEAEMT
                     AMSDRAFDALSKLLGENKYLMGSEPCGADATAFAFIAGSLCPLFDSPAHARARTLPNL
                     TAYRDRMMAEFYPGFEKGAAPIS"
     gene            complement(2023..2247)
                     /locus_tag="VEW08_06060"
     CDS             complement(2023..2247)
                     /locus_tag="VEW08_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HYJ40335.1"
                     /translation="MRTLKLKVKPGSREEALSELDDGSWLARVKAPPVDGKANAAVVA
                     LVAKHFGLRKAQVHIKSGASGRSKLVQLED"
     gene            complement(2314..>2675)
                     /locus_tag="VEW08_06065"
     CDS             complement(2314..>2675)
                     /locus_tag="VEW08_06065"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYJ40336.1"
                     /translation="DLKPGGKFRCIANEQMRSFGLPEVIVDGEVIEVKPPYKLVQTYR
                     FLFNEEHKKEGFTRISFEIEQTAGGFCRLTVTHDTAGAPMMTRATTSNFSPDGGGGWD
                     WILSDIKTLLETGKTMS"
BASE COUNT          463 a          835 c          847 g          530 t
ORIGIN      
        1 caacgtggcc gtgttcagcg tggatgcgaa gacgggagag cttgcgccga agggtgagcc
       61 cgtgccgctg ccgaaaccga tggccgtggt cttcctgaag taatggggtc aggcaccgtt
      121 tcctgggaat cggtgcctgg caccgtttac taggaatcgg tgcctggcac cgtttactag
      181 gaatcggtgc ctggcaccgt ttagtggcac cgtttagggt ttgagccacc gcacgaacca
      241 cgcgatgccg gcgtcgatgc tctcggggtt cacgcgatgc ggcgtgttct cctggatgat
      301 gagctggagt ttgtcgccgg cattcgcgga ttcgtagatg ggcttcgcgg cagcgaccga
      361 caaccgcacg ccggcaaccg gcgtgttcgc atccgaatct ccgttgatca cgagcagcgg
      421 ccgcggcgcg atggcggcga gcatcgccgg tccatcgaac tgcccgtcga tgccgggcac
      481 cacgcgcgcg tagaactcgc gcacgaaatc gacgctgcgc gcgggcttgc cggcggccgc
      541 cgctgccgca ttgaaggcat cctgaatcgt cgcgatgcgc gcggcccatt ggccgttttc
      601 cagcgcccat ttgaaactct gcacgccgat gtagggtacg accgcggcaa tgcgcggatc
      661 cgccgcggcg gtcaggtacg cctcgatgcc acccttggaa aatccggtga ggccaatgcg
      721 cgcaaagtcg acgtccttgc gcgtgccgag gtagtcgatg agccgcatga cgtcccacgt
      781 ggtgtcgtaa taaaacggat gaccttcgcc ggtcctgaac gcctgggcga tggccaagtt
      841 gtactccgcc gcacccgtgc cggccttcgt gcgttcgccg tgaaaacggc cgtcgatggc
      901 gacgccaatg aatccggcct gcactgcttt gagcacggtt gccgcgacct gttccttggt
      961 cccaccggtc ccgtgcagca cgatgaccac ggggcgcttt cccttgaagc tctcgccatc
     1021 cggcagcact agatagcccg gcacgcgctc ggtggcatca gacgagaacc acaggtgata
     1081 tttccttagc ccctcgaccg gcgccatctc ggcaagctca ggcttcaacg ccactcgcgg
     1141 ccgatcgatg acgcgcagga acgcggcgcg tgtggccgtt gcggatccat cagcaatcgc
     1201 tgcgagcgaa aactgcagcg cgaacagcgc ggctgcgagt tggcgcaaga atttcatgga
     1261 ggatgttttc attcaggcct gacccctttt gctaggaaat aggggctgca cccttttcga
     1321 agccggggta gaactcggcc atcatgcggt cgcggtacgc ggtgagattg ggcagggtgc
     1381 gcgccctggc gtgcgccgga gaatcgaaca acggacacaa cgagccggcg atgaaggcaa
     1441 atgcggtggc atcggcgccg cacggctcgc tccccatgag gtacttgttt tcgccgagca
     1501 acttcgacag cgcgtcgaag gcgcgatcgg acatcgccgt catttcggct tcctggtgac
     1561 ggccaatgcc atgcgcatgc agactgcgcc gcacatttcc gcgcaccttc cagatgacca
     1621 gcggccggat gaacgcgggt gcgcgcctga agaagttcgc gggtcccttt tcgaagttgg
     1681 cgtccttcat ccaccgccag tagaccagca cccaatacag gtggtcctcg agcatcttct
     1741 ccgtcgccca cgcgatgccc ttctcgcgcg cggagaggtt gcggtcgaag tccaccccgt
     1801 acttttcctc gagatacaac cggatcagcg tggagtccgc gacgatggtg ccgttgtcat
     1861 cgatgtacgg cagcttgcct ttcggcgcgc gcatgaagcc cttgttgctc gtctggtatt
     1921 cgagcttcgc gagcttgagc agcatttcgc ccttcatgac gaacgggctg gcatcgggca
     1981 gtccgaacaa cgggccgaag gtatacagcg tgatcacggt agttagtcct ccaactgcac
     2041 gagcttggag cggccggaag cgccgctctt gatgtgcacc tgggccttgc gaagtccgaa
     2101 atgcttcgcg accagcgcga cgactgccgc gttggccttg ccatctaccg gaggcgcttt
     2161 cacgcgcgcg agccagctgc cgtcgtcgag ctccgacaac gcctcttcgc gtgagcctgg
     2221 tttgaccttg agcttgagtg ttcgcatcgc cctgcccggt gccgcagcat tatgcggcac
     2281 cgggcaggcg atagtccgat cgaaggggcc ggcctacgac atcgtcttgc cggtctcgag
     2341 cagcgtcttg atgtcgctga ggatccagtc ccatccgcca ccaccgtcgg ggctgaagtt
     2401 ggaagtggtc gcacgggtca tcatcggcgc gccggccgtg tcgtgcgtga ccgtgaggcg
     2461 gcagaagcct cccgcggttt gctcgatttc gaacgagatg cgtgtgaatc cttccttctt
     2521 gtgttcctcg ttgaacagga agcggtaggt ctgcacgagt ttgtagggcg gcttcacctc
     2581 gatgacttcg ccgtcgacga tgacttccgg caggccgaaa ctgcgcatct gctcattggc
     2641 gatgcagcgg aattttccac ccggcttcag gtcgt
//
LOCUS       DASQJS010000148         2998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_79880, whole genome shotgun sequence.
ACCESSION   DASQJS010000148 DASQJS010000000
VERSION     DASQJS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79880"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..2022
                     /locus_tag="VEW08_06070"
     CDS             271..2022
                     /locus_tag="VEW08_06070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HYJ40337.1"
                     /translation="MTRLSTRIVIGLMLCAPVAAMAADAGFTDRIIVKYRNTSAVTAA
                     AGQATQLRGTELPAARLGVTMSRVRSTALGSQVLRLDRRLSLVEAEQLARDIVASDPN
                     VEYAEPDRIMRRTLTPNDPRYNEQWHYFEAAGGINAPPAWDKSTGTGVVVAVIDTGYR
                     PHGDLAANILPGYDFISDTFVANDGGGRDSDASDPGDWINPSECGPGDPAAFEASSWH
                     GTHVAGTIAARTNNSLGVAGVAFNARVIPARVLGKCGGFTSDIADAIIWTSGGVVPGV
                     TTLANPAKVLSISLGGSGACGSTTQNAINLARSRGASVVVAAGNSNANAANFSPANCT
                     GVVTVAAVGRNGGKASYSNFGATVEVAAPGGSGGANSVLSTLNAGTMGPGADSYALYN
                     GTSMATPHVSGVVALMLSVKPTLTPDQVTSILQGTARTFPATCSQCGSGIVNANAAVD
                     AAIGGTTPPDPPGTIAEVEPNNSRAASQLISTDNTTVNGIIGSSSDNDYYRLTLPAGG
                     TLVATLTPNATSDYDLYVYNSNGALIGSSELGTGAVDTVTVTNTGSSAFVRYVRVIRF
                     SGGTGATNGKYTVNMNW"
     gene            complement(2083..2361)
                     /locus_tag="VEW08_06075"
     CDS             complement(2083..2361)
                     /locus_tag="VEW08_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40338.1"
                     /translation="MEATMKYQLIVLCSAALASTAALAEDPDRTKGSGDLFVTLDSDS
                     DGKVSKEEAAANTSFASNFSALDKDSDGFVTKKEFRRNTMPQSKPQYR"
     gene            complement(2483..>2998)
                     /locus_tag="VEW08_06080"
     CDS             complement(2483..>2998)
                     /locus_tag="VEW08_06080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYJ40339.1"
                     /translation="AEDEARVAAEQKKVRAALDGFSSAAPATLRLEQPVPGRRSSSFG
                     LRRMFNGESRNPHSGMDIAAPTGTPIKAPLAGRVVDVGTYFFNGNNVVIDHGQGLLTM
                     YCHLSKIGVEVGQVLKRGEVLGDVGATGRVTGPHLHWGVSLNGAMVDPALFLPPPPPK
                     KKPVVTATDKK"
BASE COUNT          549 a          982 c          939 g          528 t
ORIGIN      
        1 tttttcgcgt cgcttgaatt gttcgcttca tcgtcctagg tttggttcca cgcgcaggtc
       61 gaccatggaa ggtctttgcg accctacgga agattctgca gaagttagtt gagcctcacg
      121 gccacctcgg ccgcgggcgc cgaacaggga gcagtgcagg atgcaccttt gcaacgcgca
      181 gattcgcgcc gggcgttctt cgtccgcgct ttcgtcgcta cacctggctt ttgcggaatc
      241 tcccggccaa ccaatcgtca agggagattt atgacacgtc tatctaccag gatcgtgatc
      301 ggactcatgt tgtgcgcacc ggtggccgcc atggccgccg atgcgggctt caccgatcga
      361 atcatcgtca agtaccgcaa tacgtccgcg gtcaccgccg cggcggggca ggccacgcaa
      421 ttgcgcggca cggaactgcc cgccgcacgc ctcggcgtca ccatgagtcg cgtgcgctcg
      481 accgcgctcg ggtcgcaggt gctcaggctc gatcgccggc tgtcgctcgt ggaagccgaa
      541 cagctcgcgc gcgacatcgt cgccagcgat ccgaacgtcg agtacgccga accggatcgc
      601 atcatgcgac gcacgctcac ccccaacgac ccgcgctaca acgaacagtg gcactacttc
      661 gaagcggcgg gtggcatcaa cgctccgccg gcctgggaca agtcgacggg cactggcgtg
      721 gtggtcgccg tgatcgacac gggatatcgc ccgcatggcg atctcgccgc caacatcctg
      781 ccgggttacg acttcatctc ggacacgttc gtggccaacg acggcggcgg gcgcgacagc
      841 gatgccagcg atcccggaga ctggatcaac ccgagtgaat gcggacccgg tgaccctgcg
      901 gcattcgagg cgagcagctg gcacggtacc cacgtcgccg gaacgatcgc ggcgcgcacc
      961 aacaactccc tcggcgtcgc gggcgttgcg ttcaatgcca gggtgattcc ggcgcgcgta
     1021 ctcggcaagt gcggcggatt cacctcggac atcgccgacg ccatcatctg gacctcaggt
     1081 ggcgtggtcc ccggggtgac gacgctcgcc aatccggcca aggtgctcag catttcgctg
     1141 ggcggcagcg gtgcctgcgg ctccaccacg cagaacgcca tcaacttggc gcgatcgcgc
     1201 ggcgcgtccg tggtcgtggc ggcgggcaac agcaacgcca atgcggccaa cttctcaccg
     1261 gcgaattgca cgggtgtggt caccgtcgcg gccgtcggcc gcaatggcgg caaggcctcg
     1321 tactccaact ttggcgcgac cgtggaagtg gcggcgccgg gtggcagcgg cggcgccaac
     1381 agcgtgctgt cgacgctcaa cgcgggcacg atgggtccgg gcgcggacag ctacgcgctg
     1441 tataacggca cctcgatggc cacgccgcat gtctcgggcg tggtggcgct catgctgtcg
     1501 gtcaagccca cgcttacgcc cgaccaggtg acgtccatcc tgcagggcac ggctcgcacc
     1561 ttcccggcga cgtgttcgca atgcggctcc ggcatcgtga acgccaatgc cgccgtggat
     1621 gcggccatcg gcggcaccac gccgccggat ccgccgggca ccatcgccga agtggaacct
     1681 aacaactcgc gggccgcctc gcagttgatc tccaccgaca acaccacggt gaacggaatc
     1741 atcggcagca gcagcgacaa tgactactat cgcctgacat tgccggccgg aggcacactg
     1801 gtggcgacgc tgacgccgaa cgcgacgtcg gactacgacc tatacgtgta caacagcaat
     1861 ggagcgctga tcggtagcag tgagctcggc acgggcgcag tcgacacggt caccgtgacg
     1921 aataccggct cgagcgcgtt cgtgcgctac gtccgcgtca ttcgcttcag tggtggcacg
     1981 ggcgccacca acgggaaata caccgtcaac atgaactggt gagacagtgg gggacagggc
     2041 ttcggctctg tcccccattt gcttccggtg gcgctgcagc tttcagcggt actgaggttt
     2101 cgactgcggc atcgtgttgc ggcgaaattc tttcttggtc acgaaaccat ccgaatcctt
     2161 gtccagtgct gaaaaattac tcgcgaagct ggtgttggca gccgcctcct ctttgctgac
     2221 ctttccgtcg gaatcggagt cgagggtcac gaacaagtct cccgaacctt tggtgcgatc
     2281 cgggtcctcg gcaagtgcgg cggtggacgc cagcgcagcc gaacacaaca caattagctg
     2341 atatttcatg gtggcctcca tggcatcggc atgattgacg ccataagtga cgaatggcgc
     2401 acgccgaagt tccggtgcgt gcgcgggcta acgccaaccc aagatggatt ccgacattcg
     2461 aaattgccgg cgaaagccgg tcctactttt tgtcggtcgc cgtaaccacg ggctttttct
     2521 tgggcggggg cgggggcaga aacagagcgg gatccaccat cgcgccattc agcgacacgc
     2581 cccagtgcaa gtgcggtccc gtcacgcgac ccgtggcgcc gacgtcaccg agcacctccc
     2641 cgcgcttgag aacctggccg acttccacgc caatcttcga cagatggcag tacatggtca
     2701 gtaatccctg cccgtgatcg atcaccacat tattgccatt gaagaagtag gtaccaacgt
     2761 cgaccacgcg acccgcgagc ggcgccttga tgggagtgcc ggtgggcgcg gcgatgtcca
     2821 ttcccgaatg ggggttcctc gactcaccgt tgaacatgcg ccgcaggcca aaagagctcg
     2881 aacgccggcc gggcactggc tgctcgaggc gcagcgttgc gggcgcagcg gatgaaaacc
     2941 catcgagcgc cgcgcgcacc tttttctgtt ccgcggccac gcgcgcttcg tcttcagc
//
LOCUS       DASQJS010000149        19274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_80192, whole genome shotgun sequence.
ACCESSION   DASQJS010000149 DASQJS010000000
VERSION     DASQJS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19274
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80192"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VEW08_06085"
     CDS             complement(<1..821)
                     /locus_tag="VEW08_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40340.1"
                     /translation="MRSKLLAAAALAIPMLLAGCVTSEVKPIPKVAAKQATVHIPQAE
                     LLDVGIRVFDAGIPKNLEGDDEALAKKRIYPDLRKAEARYMPTLLRATLEETAQWGAV
                     RVIPNSADFVDVIVTGKVVDSNGGFMALEISASDAAGRVWIKNKQYNSLVDLGAYKTT
                     AALKARDPFQNVYSEIANDLVLARDKLSALDRENIRKVASLRFAQDLAPDAMQGMTTV
                     DKNGITQVARLPAEGDPTLTRIERIRERDTAVVDTVNDYYASFAESMDDSYDSWR"
     gene            complement(855..1748)
                     /locus_tag="VEW08_06090"
     CDS             complement(855..1748)
                     /locus_tag="VEW08_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40341.1"
                     /translation="MRAPILISLAAAAAILAGCASSSGGMPSPPSGGGGSPSGGGGGG
                     MPGGQSGGMPGGMPGGSSGMPGGSSGMPGGSSGMPGMPGGSSGMPGMPGESSPSASMP
                     GLPGESGESGGKSGGASGSGDSDSKSGKDGEAGSASTEGPGGGAQTSEERRQAGEKSL
                     DDSLGDFDKTLKKEQERVAKERDSRGTAEGEGDSGEAGEGGEAGEGASGSGSQQRAGD
                     LKSEGNAPKSASNNSGDQSSSDQSGKGSGSGGGGGGKERVVNDDDIVAKRLRKAAEEE
                     TDPELKERLWKEYNDYKKSAS"
     gene            complement(1767..2447)
                     /locus_tag="VEW08_06095"
     CDS             complement(1767..2447)
                     /locus_tag="VEW08_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40342.1"
                     /translation="MNAIYMTTACLGAVLLGGCMQARIEESREMPTHLAKGERVVILA
                     KPQIEGAGAEDDFMDCIGENLGNGKGSLLVHDNNDFVDQMFPWFEPSTAPGKPEAMSE
                     LLARPGVADQVTKSGVRYVVWLDGSTRKTDGGGSLACGAAPGGAGCIGFGWWQKESAY
                     EATIWDLKQAKSAGSVGTNVTGTSAIVGAIVPLPFIARVQATACNRMSTQLRSFFSGT
                     DGIPAGTH"
     gene            complement(2574..3626)
                     /locus_tag="VEW08_06100"
     CDS             complement(2574..3626)
                     /locus_tag="VEW08_06100"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HYJ40343.1"
                     /translation="MRLVGGLVEHLRFFHTVDPAISRKRDIAGQAVKSEAKLGVVSIE
                     PLGKASRLYDITTGTEDFIANGVVSHNCYARPSHAYVGLSPGLDFETKLFYKADAAAV
                     LRKELAAPSYKCAPIMLGANTDPYQPLEKAHKVTRSLLEVLLEHKHPVSITTKGALVA
                     RDVDLLAQLARDGLTRVMFSIPTLDNEMKRVLEPRAASAGAKLKTMRVLAEAGVPVGV
                     LVAPIIPVLTEHEIEAVLEASREAGASLAGYTLLRLPWEVKDLFREWLAEHFPDRAAH
                     VMSIVRGMRGERDNDPSFGTRMHATGPVAQLIRQRFQLACRRLGFPPERENALPTNLF
                     RPPLRTHPQLSLDLPP"
     gene            3597..4055
                     /locus_tag="VEW08_06105"
     CDS             3597..4055
                     /locus_tag="VEW08_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40344.1"
                     /translation="MFDEATDEPHDIDGFRRYTVGVLNDEPEIQLAQRAVNAAYYFLV
                     GVGDAENAPAVASFCVENSGEETRNPLNAWSLGPRNELLHTFNAGTRVGADRVHRNSS
                     FTGGRENERSSFYALLPQVFLSSNQCLAIDQRKTELVLIAMGSAFAFVVI"
     gene            complement(4799..6031)
                     /locus_tag="VEW08_06110"
     CDS             complement(4799..6031)
                     /locus_tag="VEW08_06110"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ40345.1"
                     /translation="MISNSRSPGRQALSHPDFARYAYGRFAATLAWQMIDVVLGYQVW
                     ELTRKEEYLGLIGLAQFLPFVVLLLPGGQIADRFDRRLIIALAYGLELVAAVALLWFT
                     LSGRHEIILLFFIAALLGVGRAFWAPAGQAMVPNLVPKELLSGAISMNTLMFTIATIS
                     GPAIAGLTLILGIDWSYGITVALLIGAIFMMLGVKPVLAKSSAEWRWRDVLGGFVFVW
                     QKKPVLGAISLDLFAVLFGGVVGLLPAVADKILHVGPVGLGLLRAAPGVGAAIVAFWL
                     GMRPIQRHAGTWMFGGVALFGVCMITVGLSTSIWLTLAALIIAGAGDMISVFVRGMLV
                     QLETPDSIRGRVSAVNSMFIGASNELGVFRAGMQAAWMGIAPSMIWGGVCTLAVVGSY
                     LGLFPQLRKLDRFPEPAH"
     gene            6157..7941
                     /locus_tag="VEW08_06115"
     CDS             6157..7941
                     /locus_tag="VEW08_06115"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HYJ40346.1"
                     /translation="MAGFVAMLVCACAPTPPRADPPPPPPPPPPTADQQFEALARRYL
                     QEAPEQSPVGATSLGDHRFDDRLDDVSAAGWQARLTFTELYLAALEAIDRGNLSRANQ
                     VDALLLVHDLQGDRWKYQTLQDWRWNPLIYTRLAGDGLYNLLAREFAPAPERLKNLGK
                     RLDELPRFLAQVREVLEPARVPKVFAETAAKQNAGLKSMLEGEITAQIATLPMEEQDA
                     FKASVAKARSAVSQHQIWLEKRLTPEAKGDFRLGPELYDQKLSFALFSPLTRQQIRER
                     AESELAATRAAMYEIATVVLKGKRGSVPTPAKPSAAQQQRAIKAALELAYAERPKRDA
                     VLDTARASLVDATQFVREKNIVTVPDEPLEIIVMPEFQQGVALAYCDSPGPLEAGQKT
                     FYAVSPIPAQWTRAQTDSFLREYNTRSIHNLTIHEAMPGHYLQLAHANKYPSTLRKVL
                     ASGPFIEGWAVYTERVMIDQGYMNGDPLMKLIQLKWYLRSIVNALLDQAVHVDGISRE
                     AAMKLMTESSFQEEREAAGKWTRAQLTSAQLPVYFVGAQEHMALRAEAEQRLGAAFSM
                     REYHDKVLSYGSPPVRFARVLMFDLPIE"
     gene            complement(7957..8235)
                     /locus_tag="VEW08_06120"
     CDS             complement(7957..8235)
                     /locus_tag="VEW08_06120"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HYJ40347.1"
                     /translation="MFDKKHPFYGSYRMAKRIVIGIVGGTIVIAGIILAVMPILPGWP
                     AIFIGLGILGLEFAWARSWLRKAKEKAEDVGRSISGKPAIDRSKSSDR"
     gene            complement(8342..9862)
                     /gene="ilvA"
                     /locus_tag="VEW08_06125"
     CDS             complement(8342..9862)
                     /gene="ilvA"
                     /locus_tag="VEW08_06125"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HYJ40348.1"
                     /translation="MTDTYIERILRARVYDVATETPLEFAARLSRRIGNSVLLKREDM
                     QPVFSFKLRGAYNKIAHLSESVAKRGVICASAGNHAQGVALAARRREIPAVIVMPQTT
                     PNIKVSAVRDLGGEVVLHGDDYDSAYEHAQSIVRDRNLAFVHPFDDPDVIAGQGTIAV
                     EILRQARGEVDAIFVPVGGGGLISGIALYAKYFNPKIRIIGVEPVDAAAMHDSLRAKK
                     RVVLDRVGVFADGVAVKKVGEETFRLCREHVDEIVLVDTDEICAGMQDIFEDTRSIVE
                     PSGALAVAGIKKYCALNQWNDKRVVAINSGANMNFDQLRYVAERSDVGQAREALFAVE
                     IPEQPGSFLRFCEILGQRAVTEFNYRHGAKERAQIFVGFALARGKEERDEVIGKLKAA
                     GYGVTDMTDNEIAKLHVRYMVGGHVNHGVTSERLYRFEFPERKGALLKFLQSVGTRWN
                     ITLFHYRNHGSDYGRVLAGIDVPSSDTADFLDHLNVLHYSYSDETNNPAYRIFLGA"
     gene            complement(9886..11418)
                     /locus_tag="VEW08_06130"
     CDS             complement(9886..11418)
                     /locus_tag="VEW08_06130"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HYJ40349.1"
                     /translation="MPPVNTSASPSDVDNEATAELPVLDVAAYESTLNDPISHTDSWV
                     LPTGAQTQTASVSAEATLQLPVMSSAPKPAYDIDHSGTHEMPQMSLSVPNSARKAARP
                     SAVPTPAPASAPVITATPTHPVASSPVQITLPPSPPLIEELRRSLATAERRIEELNER
                     ARIADAERGVAVARANAETAQLREQLAAHLESLHTTKERLGVQRADIPELEDQLYARA
                     DRIAALEKALAADAAVLADHAARLANSEQRCEVLECESMNLRATVARRNAQISTLEAD
                     LAARQQRESQISARLAEALASTDPYVPQLRSELHALDEQIRLLKLDVEIARSHAAEKD
                     HDLQVAEECIRNLEIDVRDKTSKLEEANVTVEEWRAVIAESQRSILQRDNQIQQLQSD
                     LEKRIASVRDSAAQTGEEVALEGPARVLIRTDGNTEFVHVLGRRTRIGRGSDNELVLD
                     TKHISRYHAVLLAGPVHTSIEDLNSTNGVFVNGKRVSRQVLKDGDRVTVGRTQFRYTV
                     RD"
     gene            11596..12174
                     /locus_tag="VEW08_06135"
     CDS             11596..12174
                     /locus_tag="VEW08_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ40350.1"
                     /translation="MDTAPPRLRRAYFECRHGQLHVHNAIPVGGGFDELTTLICLHGA
                     NGTGRMFLEVSKRLGSSRSIYSPDLPGCGESDASTTRLSIGGFADAIADFLDSMRFRQ
                     VDLLGVGAGAAVAVELAVSRPKVVRKLYLLGVAGRETAAPGPDAQALQAAAADWHADR
                     RQLVKQPVLALEDESVETPALAGKIAEFFAGH"
     gene            complement(12168..12911)
                     /locus_tag="VEW08_06140"
     CDS             complement(12168..12911)
                     /locus_tag="VEW08_06140"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HYJ40351.1"
                     /translation="MKTGEIQRFNEFGQPIGFDLPGWKPPPFPPHATLQGRYCRIEPL
                     NAAKHARDIYEAQREDTDGARWTYLFHGPYDNFDDYEKWALGAEASRDPQFYAIVDAA
                     TARAVGSCAYMRIEPQHGVIEVGNIYFSPRLGRTRAATEAMYLLLANAFDLGYRRYEW
                     KCDSCNLPSRAAATRFGFTYEGLFRQAIVNKGRNRDTTWYAIIDRDWNGGLEDAYLRW
                     LDPANFDAAGTQRQKLSGLTAPHVHVLSQ"
     gene            13023..15059
                     /locus_tag="VEW08_06145"
     CDS             13023..15059
                     /locus_tag="VEW08_06145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYJ40352.1"
                     /translation="MNRSAILLLALVFSTSAFAADDSRLLTAEKMWALKRLGDPAITP
                     DGLAAVLPVTTFDIAENKGLTDLWLIPLPGGPGRQLTSDKANDTQPTVSPDGKWVAFV
                     SKRGDDAESQIYVIAVDGGEARRVTNVPTGASVPKWFPDSSRLAFVSEVWPDLVRWED
                     QGARKKQRADSKMTARVWTKSPISYFDHYLDDREPHLFSIARDGGEPAAITRMSGYHL
                     SKQEYDASSYDISPDGLEMAFAAETDRTGIDSNFDLILLETCGCKPPRDISTANKADE
                     GSPRYSPDGRRLAFTQQRERRFYADRARLMIYDRAAGTTVGVTENWDRTVDGLVWERD
                     SRTLLGSIDDAATRRIYRFRLDGGAPVAVTKASSFSSLALSGNGKALVGIRQSFSEPP
                     TLVSIAPRTGAATKLSTFNDTALASFDQGKVESVVYKGARDADIQMWVVYPPGFDSTK
                     KYPAMMLLHGGPHSGITDAVQWRWNAQVFASWGYVVTWHNFHGSSGFGNDFTDSINPD
                     QITMPYEDTIKAAGWLMAKPFIDKERMVAAGGSYGGFLAATLLGRDHPFKAMVAHAAV
                     YNRFTQIGADYGAETERFFNFWDKPEEFARYSPHSSAGNFKTPTLVIHNQNDLRVPLN
                     HGIELFNTLQKRGVPSKLVYFPDENHWVLKPQNSLFWYQTVRDWVATYAKPGPY"
     gene            15074..16180
                     /locus_tag="VEW08_06150"
     CDS             15074..16180
                     /locus_tag="VEW08_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYJ40353.1"
                     /translation="MKRRTLLALTTAGAAALALPSWAARKPKPLKILILGGTRFIGLH
                     MTALALKRGHTLTFFNRGKTKTDRYPEIERIKGDRNGEIDGLKDREWDVVIDNSGYVP
                     RHVRLSAELLAPKVKQYVFVSSISVYPDFSVPRDEKSPVGKLADETVEKVDGETYGPL
                     KALCEQAVEKALPGRTTIIRPGLIVGPDDNTDRFTYWPARAARGGEFIAPGAPSDAFQ
                     VIDVRDLAAFTINAVENNFIGTYNLVSNVNEFKFGELTDACVAAARKQAKPADAPRAT
                     YLPTAFLEEQKVEPWSEMPVWLPAKGDETAFAGTSNKAAKARGLKITPIKKTVDDTLA
                     WHLTRPEEERVKLKAGIAPEKEAAVLAAWKAKKA"
     gene            complement(16183..16659)
                     /locus_tag="VEW08_06155"
     CDS             complement(16183..16659)
                     /locus_tag="VEW08_06155"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ40354.1"
                     /translation="MDKPSLNIVRAADIAAHSQEFSHPWNPNSELHGTQLARSVGLKR
                     TGVNFIRVPAGKESYVYHSHQCEEEWIYVLSGKAIALIDDVEYEVNAGDFIGFPAPSV
                     AHHMRNPGPADLLYLAGGENRDFEVADFPKLGKRMVRSGKQLDIYDIADAQPFGPL"
     gene            complement(16728..16934)
                     /locus_tag="VEW08_06160"
     CDS             complement(16728..16934)
                     /locus_tag="VEW08_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40355.1"
                     /translation="MQGLGGFLVLMGAGSFVLHYMDMEFTLLSWVDNWGLGIGNGIRI
                     ALIVVGGIIWFLGKNSAEKEAPPA"
     gene            complement(17018..17359)
                     /locus_tag="VEW08_06165"
     CDS             complement(17018..17359)
                     /locus_tag="VEW08_06165"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HYJ40356.1"
                     /translation="MSDPVTGWALAVVIAGLALSTFVTRTSFLIAGAKLRLGARVEVA
                     LRYAPVCTLAAIIVPDILIHPPGAAIDLSLLNPRLIGALAAGLWLCWSRNILGCLGLG
                     MIAYTLARLYL"
     gene            complement(17356..18087)
                     /locus_tag="VEW08_06170"
     CDS             complement(17356..18087)
                     /locus_tag="VEW08_06170"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HYJ40357.1"
                     /translation="MVSAQNLERRAAFRLGQRAILPSLPGAFAWGMVSGVAMVKGGLA
                     VHWAIMMSLLVYAGSAQLAALPLIVAGAPLWVIVATGLITNLRFVIYSAALRPHFANL
                     PWRRRAALGFFMTDFTFTRFMRSAQEGALPEHRDAWFAGVCANNWITWQLSAITGIIG
                     ASYVPTDWGLEFTGTLALVALVGPSLNARPAIVGAAAAAVVALIAYPMPFKLGLFCGA
                     IAGIVAATLTDVAQQRSPPQSAEDT"
     gene            complement(18081..18500)
                     /locus_tag="VEW08_06175"
     CDS             complement(18081..18500)
                     /locus_tag="VEW08_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ40358.1"
                     /translation="MKYLHTMVRVTDVDASLKFYCDALGLEVVKRTDYPQGKFTLIFL
                     AAPGDHTAQVELTHNWEPEAYGGGRNFGHLAYAVDDIYATCERLQKHGVTINRPPRDG
                     RMAFVRSPDNISIELLQKDGSLPPREPWVSMANTGVW"
     gene            complement(18515..>19274)
                     /locus_tag="VEW08_06180"
     CDS             complement(18515..>19274)
                     /locus_tag="VEW08_06180"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ40359.1"
                     /translation="HGRRAGFKRDRSKWLAYLADLRRTYAMNYDRDSEKIQPYYVIEE
                     LNRVTKGEAIISTGVGQHQMWAAQYCDFRNPRQWLTSGSMGTMGFGLPAAIGAQFARR
                     DKLVIDIDGDASVRMNFGELETVTTYDLPVKVVILNNNGDGMVKQWQKLFFKGRLSAS
                     EKTLHTKDFIKAAQADGFKYAVRLDRKADVPRVIEEFLAFKGAAFLEVIIDPDAGVYP
                     MVGPGQPYEAMITGDWIPSRKKVDVKPPGASEMF"
BASE COUNT         3527 a         6070 c         6114 g         3563 t
ORIGIN      
        1 tggcgccagc tgtcgtaaga gtcgtccatc gactcggcga agctcgcgta atagtcgttg
       61 accgtgtcga ccaccgcggt gtcgcgttca cgaattcttt cgatgcgggt gagcgtgggg
      121 tcgccctccg cggggaggcg cgccacctgc gtgatgccgt tcttgtccac ggtggtcatg
      181 ccctgcatgg catcgggtgc gagatcctgg gcgaaccgca ggctggcgac cttgcggatg
      241 ttctcgcgat cgagcgccga gagtttgtcg cgcgcgagca cgaggtcgtt ggcgatttcc
      301 gagtagacgt tctggaaagg atcgcgcgcc ttgagcgcgg cggtggtctt gtacgcgccg
      361 agatcgacca ggctgttgta ttgcttgttc ttgatccaca cgcgccctgc cgcatcggac
      421 gcgctgattt ccagcgccat gaagccgcca ttcgaatcga ccaccttgcc ggtgacgatg
      481 acgtcgacga aatccgcgct gttcgggatg acgcgcaccg caccccactg cgcggtttct
      541 tcgagcgtcg cgcgcagcag cgtcggcatg tagcgcgctt cggccttgcg cagatccgga
      601 taaatgcgtt tcttggcgag agcttcgtcg tcgccttcga gattcttggg aatgcccgca
      661 tcgaacacgc gaatgccgac gtccagcagt tcggcttgcg ggatgtgcac cgtggcctgc
      721 ttggccgcga ccttcgggat tggcttgacc tcggaagtga cgcatccggc gagcaacatc
      781 ggaattgcca gtgccgccgc cgcaagtagt ttggatctca tgacgtcacc ttcgtcgatt
      841 cctctgggat cgaatcaact cgccgacttc ttgtagtcgt tgtattcctt ccagagccgc
      901 tctttgagct ccggatcggt ttcctcttcc gcggccttgc gcaaacgctt ggcgacgatg
      961 tcgtcgtcgt tgacgactcg ctctttcccg cctccaccgc cgccactgcc tgagcccttg
     1021 ccgctctggt ctgaggaaga ctggtcacca ctgttgttgc tggcggactt cggagcattg
     1081 ccttcggatt tcaggtcgcc ggcacgctgc tggctaccgg agccgctggc gccttcccca
     1141 gcctcgccgc cttccccagc ctctccggaa tcaccttcgc cctcggccgt gccgcgcgaa
     1201 tcgcgttctt tcgcgacccg ctcctgttcc ttcttgagcg tcttgtcgaa atcgccgagc
     1261 gaatcgtcga ggctcttttc gcccgcctgc cgccgttcct cggaagtttg cgcgccaccg
     1321 ccgggacctt cggttgaagc gctgcccgcc tcaccgtctt tcccggattt cgaatccgaa
     1381 tcgccggaac cgctggctcc acccgatttc ccaccggact cgcctgactc accaggcaac
     1441 ccgggcatgg atgcggaggg agatgattcg ccgggcattc caggcatgcc ggaactgcca
     1501 ccgggcatgc caggcatgcc actgctgccg ccaggcattc cgctgctgcc accgggcatc
     1561 ccgctgctac cgccgggcat tccacccggc atgccgccgc tttgaccacc gggcattccg
     1621 ccgccgcctc caccgctggg cgaaccgccg ccgccactcg gagggctcgg cataccgccg
     1681 ctggaggacg cacaccccgc gaggatcgct gctgcggcag cgagggaaat caggattgga
     1741 gcgcgcatgc gaatctcctg ccgtaatcag tgggtaccgg cgggaatacc gtcggttccc
     1801 gagaagaaac tgcgcagctg ggtcgacatg cgattgcatg cggtggcctg cacccgcgcg
     1861 atgaatggca acggaactat tgccccaacg atggcggaag ttccagtgac atttgtaccg
     1921 acgctacccg ctgatttggc ttgtttcaaa tcccagatag tcgcctcgta agccgattcc
     1981 ttctgccacc agccaaagcc gatgcagccc gccccgccgg gggccgcgcc acaggccagg
     2041 ctgccgccac catcggtctt gcgggtgctg ccgtcgagcc agactacata acgcacaccc
     2101 gacttcgtga cctggtcggc cacgccaggg cgcgcgagaa gctcactcat ggcttcgggc
     2161 ttgccggggg cggtgctcgg ctcgaaccag ggaaacatct ggtcgacgaa atcgttgttg
     2221 tcgtgaacca gcaggctgcc cttgccgttg ccgagatttt cgccgatgca atccatgaag
     2281 tcgtcttccg cgcctgcgcc ttcgatttgc ggcttggcga ggatgaccac gcgttcgccc
     2341 ttggcgaggt gagtgggcat ttcacgcgat tcttcgatac gagcctgcat gcatcccccg
     2401 aggagaacgg cacccaggca cgcagtagtc atgtagatcg cgttcatgga cgaactccca
     2461 gggcctcgat gcgggatgtc tatgggcgca gcaagtatgc cacgtcgcag caccgtcact
     2521 ttgagacctc gcccgccgct ccggggttcc cgactccgcg tgggaccatc gacctacggc
     2581 ggcagatcca gcgacaactg agggtgagta cgtagtggcg ggcggaaaag attcgttggc
     2641 agcgcatttt cccgctccgg cggaaagccg aggcgccggc aggcgagctg gaaccgctgg
     2701 cgaatcagct gcgccacggg tccggtggcg tgcatgcgcg tcccaaagct ggggtcgttg
     2761 tcgcgttcgc cgcgcatgcc acgcacgatc gacatcacgt gcgcggcgcg gtcggggaaa
     2821 tgctcggcca gccattcacg gaacagatct ttcacttccc agggaagtcg taacaatgtg
     2881 tatccggcga gcgacgcgcc ggcctcgcga gacgcttcga ggacggcttc gatctcgtgt
     2941 tcggtgagca cgggaatgat gggcgccacc agcacaccca cgggcacacc cgcttccgcc
     3001 agcacgcgca ttgttttcaa ttttgccccg gcggaagccg cgcgcggttc gagcacgcgt
     3061 ttcatctcgt tgtcgagcgt agggatgctg aacatcacgc gcgtgaggcc atcgcgtgcg
     3121 agctgcgcca gcaggtcgac gtcgcgcgcg accagcgcgc ccttggtggt gatgctcacc
     3181 ggatgtttgt gctcgagcag cacctcgagg agcgaacgtg tgactttgtg tgccttctcg
     3241 agcggctggt acggatcggt gttggcgccg agcatgatgg gcgcgcattt gtagctgggc
     3301 gcggcgagtt cttttctcag caccgccgcg gcgtcggctt tgtaaaaaag tttggtttcg
     3361 aaatcgagac cgggagagag gccgacgtag gcgtggctgg ggcgggcgta gcagttgtga
     3421 ctgacgacgc cgttagcgat gaagtcttcg gttcctgtag tgatgtcgta gagccgcgaa
     3481 gcctttccga ggggctcgat gctgaccact ccgagtttcg cttcgctctt gacggcctgg
     3541 ccggcaatgt cgcgcttgcg ggagattgcg gggtcgacgg tgtgaaagaa ccgcaggtgt
     3601 tcgacgaggc caccgacgag ccgcacgaca tcgatgggtt tcgtcgctat acggtcggtg
     3661 tgctcaatga cgaacctgaa attcaactcg cgcagagagc ggtcaatgca gcctattatt
     3721 tccttgtcgg tgttggagat gcggagaatg ccccggctgt agcttccttc tgcgtcgaaa
     3781 attccggcga ggaaacccgc aatccactca atgcttggag ccttgggcca cgcaatgagt
     3841 tgcttcacac cttcaacgcc ggcacgcgcg tgggtgcgga tcgcgttcat cgcaattctt
     3901 ctttcacggg cggccgcgaa aacgaacgat cgagtttcta tgctctcttg cctcaggtat
     3961 tcctgagttc gaatcaatgc ctcgctatcg atcagcgcaa gacggaattg gtgttgatcg
     4021 ccatgggctc ggccttcgcg ttcgtagtga tatgaagcta gcaggccgtc accgcgaatt
     4081 acgccgcaga ggtaaccgcg acggtactca ggacctttgt ttggacctgc ggcaaaaccc
     4141 cccgttccca tcagcttgtt gttgttggtc aggaacgggc gttgggaatt tccgctttca
     4201 gtcccagtaa cgaatttcca gcctcggtcc gtcaggaatc gatgatcagg cccggcgacc
     4261 agtgtcgtgc catcttcgag cgtaatccga tatgcaggct tgatgacgct ccaatgagcg
     4321 agaactcgcg tcttcacgta tcggcgatac cagccaattc tttcggtgcc gtagatcgca
     4381 tcaccaactt tcatttcggc gatgactttc gttgtgccgt ccgccatgag tatcggcgta
     4441 tcgccccaca ggcagtacac acagccgtgt tcacatcctc gatatggatt gatggactgg
     4501 gagaaatgga tgtcgggtga ttgattgcgg ctgatgatcg agcgggcatg ttcaggcaac
     4561 accacggtct cgcgcttctc cggctgttct tcttcgtacc agccgtcgtg ctcgagagtt
     4621 tgtgtgagtt tgtcgaagcg gccgggcggc tgagacagcg cgcctcggcc tttgatcgcg
     4681 cttgccatgc gcgaactgta tttacgtaca gggcgcgagt aaatccagta cgaccaccgg
     4741 tccgacactg tatgaacgcc gccgcttcgg cggcgatttc cgacgtgggc gtcagccctc
     4801 aatgagccgg ttcggggaag cggtccagct tgcgcagctg ggggaacagg ccgagataac
     4861 taccgaccac cgcgagcgtg cacacgccgc cccagatcat cgacggcgcg attcccatcc
     4921 acgcagcctg catgccggca cggaacactc cgagctcatt ggaggcgcca atgaacatgg
     4981 aattgactgc gctcacccga ccgcggatcg agtccggcgt ctccagctgc acgagcatcc
     5041 cgcgcacgaa cacgctgatc atgtcgccgg cgccggcaat gatcagcgcc gcgagcgtca
     5101 gccaaatgct ggtcgacagc cccacggtga tcatgcagac gccgaacaac gcaacgccgc
     5161 cgaacatcca ggttcccgcg tgccgctgga tcggtcgcat accgagccag aacgcgacga
     5221 tggcggcgcc cacgccgggc gcggcacgca gtaatccgag gcccacggga ccgacgtgga
     5281 gaatcttgtc cgcgaccgcc ggcaagagac cgacgacgcc gccgaacagc acggcgaaaa
     5341 gatcgagcga gatcgcgccg agtaccggct tcttctgcca gacgaatacg aatccgccga
     5401 ggacgtcgcg ccagcgccat tccgctgacg acttggcgag caccggcttg acgccgagca
     5461 tcatgaaaat cgcaccgatc agcagtgcaa ccgtgatccc gtacgaccag tcgatgccga
     5521 ggatcagcgt aaggccggcg atggccgggc cgctgatggt cgcgatggtg aacatcagcg
     5581 tgttcatgga aatggcgccg gacagcagct ccttcggcac caggttcggc accatcgcct
     5641 ggcccgcagg cgcccagaag gcgcggccca cgcctaacaa ggcggcgatg aagaacagca
     5701 ggatgatttc atggcggccg gagagggtga accacagcaa ggccaccgcc gcgaccagtt
     5761 cgagtccgta tgcgagtgcg atgatgagcc ggcgatcgaa gcggtcggcg atctgtccgc
     5821 cgggcagaag caacacgacg aacggcagga actgcgcgag gccgatgagc cccagatatt
     5881 cctctttccg ggtcagctcc cacacctggt agccgaggac cacgtcgatc atctgccagg
     5941 ccagcgtcgc ggcgaatcgg ccatacgcgt agcgcgcgaa atcgggatgg ctcagtgcct
     6001 gtcggccagg cgatctggag ttgctgatca aggtcgtcgg gtggaaaacg agaggtgttg
     6061 ggagatgtgc actttaactg gtaacgtcgc gatccgttgg ccattgcgcc cggatctgcg
     6121 agaggctctg cgatgaagac ggcatctgga actgtcgtgg ctggattcgt ggcgatgctg
     6181 gtgtgcgcgt gcgcgcccac accgccgcgc gctgatccgc cgccacctcc gccgccgcca
     6241 ccacccacgg ccgaccagca attcgaagcg ctggcgcggc gctacctgca ggaagctccg
     6301 gaacaatcac cggtcggcgc gacgagtctc ggcgatcacc ggttcgacga tcgtctcgac
     6361 gatgtcagcg ctgccggctg gcaggcgcga ctcacgttca ccgagctcta tctagcggcg
     6421 ctcgaggcca tcgaccgcgg gaatctgtcg cgcgccaacc aggtcgacgc gctgctgctg
     6481 gtccacgatc tccagggtga ccggtggaaa taccagacac tgcaggactg gcgctggaat
     6541 ccgctgatct acacgcgcct cgccggcgac ggcctgtaca acttgctggc gcgggaattc
     6601 gctcccgctc ctgaacgtct aaagaacctg ggcaaacggc tcgacgagct gccgcgcttc
     6661 ctggcgcagg ttcgcgaggt gctggaaccc gcgcgtgttc cgaaagtatt cgcggaaacc
     6721 gccgcgaagc agaatgccgg attgaaatcg atgctcgagg gcgagatcac ggcgcagatc
     6781 gccacgcttc cgatggagga gcaggacgca ttcaaggcca gcgtggccaa ggcacgcagc
     6841 gccgtctcgc agcaccagat ctggctcgaa aagcgtctca cgcccgaagc caagggtgat
     6901 ttccgacttg ggcctgagct ctacgaccag aagctgtcgt tcgcactgtt ctcgccgctg
     6961 acgcggcagc agatccgcga gcgcgcggag agtgagctcg cggccacgcg cgcggcgatg
     7021 tacgaaatcg ccaccgtcgt tctcaagggc aagcgcggca gcgtaccgac tcccgcaaag
     7081 cccagcgccg cgcaacagca gcgggccatc aaggccgcac tcgagctcgc gtatgcggaa
     7141 cgcccgaaac gcgacgcggt gctcgacacc gcacgcgcct cgctggtcga tgccacgcag
     7201 ttcgttcgcg agaagaacat cgtgacggtg cccgatgaac cgctcgagat catcgtgatg
     7261 cccgagttcc agcagggagt ggcgctggcg tactgcgatt cacccggccc actcgaagcg
     7321 ggccagaaaa ctttctacgc ggtctcgccg attcccgcgc agtggacgcg cgcgcagacc
     7381 gattcgttcc tgcgcgagta caacacgcgc tcgatccaca acctcaccat ccacgaagcg
     7441 atgccggggc attacctgca gctcgcgcac gccaacaagt atccgtccac actgcgcaag
     7501 gtgctggcgt ccggcccgtt catcgagggc tgggcggtgt acaccgaacg cgtgatgatc
     7561 gaccagggct acatgaatgg cgatccgctc atgaagctca tccagctcaa gtggtacctg
     7621 cgcagtatcg tcaacgcctt gctcgaccag gcagtgcacg tcgatggcat ctcgcgcgag
     7681 gcggccatga aactcatgac ggaatccagc ttccaggagg agcgcgaggc ggcgggcaag
     7741 tggacgcgcg cgcagctcac ctcggcccaa ctaccggtct acttcgtcgg cgcgcaggaa
     7801 cacatggcat tgcgcgccga agcggagcag cgcctgggcg ccgcgttctc gatgcgcgag
     7861 tatcacgaca aggtgctgtc gtacgggtcg ccgccggtgc gtttcgcgcg cgtgctgatg
     7921 ttcgacctgc cgatcgagtg accgtccaac gattgtctat ctatctgatg acttgctgcg
     7981 gtcgatagcg ggcttgccgc tgatgctgcg gccgacgtct tcggcttttt ccttcgcttt
     8041 cctgagccag cttcgcgccc aggcgaattc caggccgaga atgccgaggc cgatgaaaat
     8101 tgccggccag cccggcagga tgggcatgac cgcgagaatg attccggcga tgacgatggt
     8161 cccgccgacg atgccgatga cgatgcgctt cgccatgcgg tacgacccat agaagggatg
     8221 ctttttgtca aacacgagca gagacctgat tgcggcggcc gggggcattc tacacagaca
     8281 ggtatgactg cccggcgttc ccccgactcc cgactatttc cttaccggga atccccggcg
     8341 atcaggcacc gagaaaaatg cggtacgccg gattgttagt ctcatcgctg taggaatagt
     8401 gcaggacgtt caggtgatcg aggaaatcag cggtgtcgga cgaaggcacg tcgattccgg
     8461 cgagcacacg tccgtagtcg gaaccatggt tgcggtagtg gaacagagta atgttccagc
     8521 gcgttcccac ggactgcagg aatttcaaca gcgcgccctt gcgctccgga aattcgaaac
     8581 ggtagagccg ctcgctggtc accccatggt tgacgtggcc gccgaccatg taacgcacgt
     8641 gcagtttcgc aatctcgttg tcggtcatgt cggtcacgcc gtagcccgcg gccttcagct
     8701 tcccgatgac ctcgtcgcgc tcttccttgc cgcgcgccag cgcgaagccc acgaaaatct
     8761 gcgcgcgttc cttcgcgcca tggcggtagt tgaattcggt gacggcgcgc tgcccgagga
     8821 tttcgcaaaa cctgagaaag ctgccgggct gctccggaat ttccaccgcg aacagcgctt
     8881 cgcgcgcctg gcccacgtcg gagcgctccg ccacgtagcg caactggtcg aagttcatgt
     8941 tcgcgccgct gttgatggcg actactcgtt tgtcgttcca ctggttgagc gcgcagtact
     9001 tcttgatgcc ggcaacggcc agagcgccgg acggctcgac gatggaccgc gtgtcttcga
     9061 agatgtcctg catgccggcg cagatttcgt cggtgtcgac cagcacgatt tcatcgacgt
     9121 gctcccgaca cagccggaac gtttcttcac ccaccttctt gaccgcgacg ccgtcggcaa
     9181 acacgccgac gcggtcgagc accacgcgct tcttcgcgcg cagcgagtcg tgcatggccg
     9241 cggcatccac gggctcgacg ccgatgatcc tgatcttcgg gttgaaatat ttcgcgtaca
     9301 gggcaatgcc cgagatgagc ccgccgccgc ccacgggcac gaagatcgcg tccacttcgc
     9361 cgcgcgcctg gcgcaggatc tccaccgcaa tcgtaccctg cccggctatc acatcggggt
     9421 catcgaacgg atgcacgaag gcgaggttgc ggtcgcgcac gatggactgc gcgtgttcgt
     9481 acgcgctgtc gtaatcgtcg ccgtgcagca ccacttcacc gccgagatcg cgcaccgcgc
     9541 tcaccttgat gttaggcgtg gtctgcggca tcacgatgac tgccggaatt tcgcgccgcc
     9601 tggccgccag ggccacgccc tgcgcatggt tgcccgccga cgcgcagatg acgccgcgct
     9661 tcgccaccga ttccgacagg tgcgcgatct tgttgtaggc gccgcgcagc ttgaacgaga
     9721 acaccggctg catgtcctcg cgcttcagca gcacggagtt gccgatgcgg cgggagaggc
     9781 gcgcagcgaa ttccagtggt gtttccgtgg caacgtcgta gacgcgggca cgaaggatgc
     9841 gttctatgta agtgtcggtc atggcgaccg acatattagc gtcactcagt ctctaactgt
     9901 gtaacggaac tgggtgcgtc cgacggtgac ccggtcgccg tccttcagga cctggcgcga
     9961 cacgcgtttg ccgttcacga acacgccatt ggtgctgttc aggtcctcga tcgaggtgtg
    10021 caccggcccg gccagcagca cggcgtgata gcggctgatg tgtttggtgt cgagaaccag
    10081 ctcgttgtcg gagccgcggc cgatgcgcgt ccggcggccg agcacgtgca cgaattccgt
    10141 gttgccgtcg gtgcggatga gcacgcgcgc gggtccttcg agcgccactt cttcgccggt
    10201 ctgcgccgcg gaatccctga ccgaggcgat gcgcttttcg aggtccgact ggagctgctg
    10261 aatctggttg tcacgctgca ggatggatcg ctggctttcg gcaatcacgg cgcgccactc
    10321 ctccaccgtg acgttggctt cttcgagctt ggaggtcttg tcgcgcacgt cgatttcgag
    10381 gttgcgaatg cactcttccg ccacctggag gtcgtggtcc ttttccgccg cgtgggatcg
    10441 cgcgatttcc acgtccagct tgagcaggcg aatctgctcg tcgagcgcat ggagctcgga
    10501 gcgcaactgc ggcacgtacg ggtcggtgga ggccagcgct tcggccaggc gcgcgctgat
    10561 ctgcgactcg cgttgctgcc gcgccgcaag gtcggcttcg agagtcgaga tctgcgcgtt
    10621 gcgccgcgcg acggtcgcgc gcaggttcat gctttcgcat tcgagaactt cgcagcgctg
    10681 ttcgctgttc gcgagccggg cggcatggtc cgcgagcacg gcggcatccg cggccagggc
    10741 tttctcgagc gcagcgatgc gatccgcgcg cgcatatagc tggtcttcga gctccggaat
    10801 gtccgcgcgt tgtacgccga ggcgttcctt cgtggtgtgt aatgactcga ggtgcgcggc
    10861 cagttgctcg cgcagttggg cggtctcggc gttggcgcgc gccaccgcga cgccgcgttc
    10921 cgcgtcggcg atacgtgcgc gttcattgag ctcttcgatg cgtcgctcgg cggtcgcgag
    10981 tgacctgcgc aactcttcga tgagcggcgg cgaaggcggc agtgtgattt gcaccggcga
    11041 cgatgccacc gggtgtgtcg gcgtggccgt gatgaccggg gcggacgccg gcgcgggtgt
    11101 gggaaccgcc gaaggcctgg cggctttcct tgcggaattg ggcaccgaca aggacatctg
    11161 gggcatctcg tgcgtgccgc tgtgatcgat gtcgtaagcc ggcttggggg ccgacgacat
    11221 cacgggcaac tgcagggtgg cttcagcaga aacagaggcc gtctgggtct gcgcgcccgt
    11281 cggcagaacc catgaatcgg tatgcgagat gggatcgttc agcgtggact cgtacgcggc
    11341 aacgtccaga accggcaatt cagccgtggc ttcattgtcg acgtccgacg gggacgcgct
    11401 ggtattcacc ggaggcatcg cggggttccc caattcttcg atttctgcgg ggattctagc
    11461 ccccgccccg cggcggatgg ttgaagactt ttcgggtacc tcgggaaatg cgaatgtgct
    11521 tctcagttct gtcaaaaccc cctaatcagg gcgccgaatc ttcgaaagcc cgacattggg
    11581 ttatggttgc gtcctatgga caccgcccca ccgcgcctgc ggcgcgcgta ttttgagtgc
    11641 cgccacggcc agcttcacgt gcataacgcc atcccggtgg gtggcgggtt cgacgagctg
    11701 accacgctca tctgcctgca cggagccaat ggaaccggcc ggatgttcct ggaagtctcg
    11761 aaacggctcg gaagcagccg ctcgatctac agcccggacc tgcccggctg cggcgaatcc
    11821 gacgcctcga ccacgcggct ttcgatcggc gggttcgccg atgccatcgc cgatttcctc
    11881 gattccatgc gcttccgcca ggtcgacttg ttaggcgtgg gcgccggcgc cgcagtggcg
    11941 gtcgaactcg ccgtgagccg gccgaaggtg gtgcgcaaac tgtacctgct gggtgtagcc
    12001 ggcagggaaa cagcggcccc ggggcccgac gcccaggcat tgcaggccgc cgctgccgat
    12061 tggcacgccg accgccggca actcgtcaaa caacccgtgc tcgcgcttga ggacgaatcg
    12121 gtcgagacgc cagcgctggc cggcaagatc gcggagtttt ttgccggtca ctgactcagg
    12181 acgtgcacgt gcggtgccgt gagtccggag agtttctgcc tttgggtgcc ggccgcatcg
    12241 aaattggcgg ggtcgagcca ccgtagatag gcgtcctcga gtccaccgtt ccagtctcgg
    12301 tcgatgatcg cgtaccaggt ggtatcgcga ttgcggcctt tgttgacgat ggcctggcga
    12361 aacagccctt cgtaagtgaa accgaaacgc gtagctgccg cgcgcgacgg cagattgcag
    12421 ctgtcgcatt tccactcgta acggcgatat cccaggtcaa aggcattcgc caacaggaga
    12481 tacatcgctt cggttgccgc gcgcgtacgc ccgaggcgcg gcgaaaaata aatattgccg
    12541 acttcgatga ccccatgttg gggctcgata cgcatgtagg cgcaggaacc cacggcgcgc
    12601 gccgtagccg catcgacgat ggcgtagaac tgcggatcgc gcgacgcttc cgcacccagc
    12661 gcccactttt cgtagtcgtc gaagttgtcg tacggtccgt ggaacaaata tgtccagcgc
    12721 gcgccgtcgg tgtcctcgcg ctgtgcttcg tagatatcgc gcgcgtgttt ggcggcgttc
    12781 agcggctcga tccggcagta gcggccctgc aaggtcgcgt gcggcggaaa cggcggcggc
    12841 ttccagccgg gcaaatcgaa gccaatcggc tggccgaatt cgttgaagcg ctgtatttca
    12901 cccgttttca tgagtgcgat tctcgcacca cgcacgggac caagcgaagg gacactacgg
    12961 caaggcgatc gggaaaagct tgcctcgaca aatcgaatcc gcagaatgcg gaaatgaaaa
    13021 acatgaatcg ttccgcaatt ctcctgttgg ctctggtttt ctcgacttcg gccttcgcgg
    13081 ccgacgactc gcgactgctc accgccgaaa aaatgtgggc actcaaacgg ctcggcgatc
    13141 cggcgatcac gccagacggg ctggctgcgg tgttgccggt gaccaccttc gacatcgcgg
    13201 agaacaaggg gctcaccgac ctgtggctga ttccattgcc gggcggcccc ggccgccagc
    13261 tcaccagcga caaagccaac gacacgcagc ccacggtcag ccccgatggc aagtgggtcg
    13321 ccttcgtatc caagcgcggt gatgacgccg aaagccagat ctacgtcatc gcggtcgatg
    13381 gcggcgaagc gcgccgggtc accaatgttc cgaccggcgc ctcggtgccg aagtggttcc
    13441 cggacagcag caggctggcg ttcgtcagcg aagtctggcc cgacctggtt cgctgggaag
    13501 atcagggggc gcgcaagaaa cagcgtgccg attccaaaat gaccgcgcgc gtctggacga
    13561 aatcgcccat ctcatacttc gatcactatc tcgatgatcg tgaaccgcat ctgttttcca
    13621 tcgcgcgcga cggcggcgag ccggcggcca tcacgcgcat gtccggttac cacctgtcga
    13681 agcaggaata cgatgcgtct tcctacgaca tttctcccga tgggttggaa atggcttttg
    13741 cagcggagac cgaccgcacc ggcatcgaca gcaatttcga tctcatcctg ctcgagacct
    13801 gcgggtgcaa gccgccgcgc gacatctcca cggccaacaa ggcggatgaa gggtcgccgc
    13861 gctacagccc ggacggtcgg cggctcgcgt tcacgcagca gcgcgaacga cgtttctacg
    13921 ccgatcgcgc acgcttgatg atctacgacc gcgccgccgg aaccacggtg ggtgtcacgg
    13981 aaaactggga ccggaccgtg gacgggttgg tctgggaacg cgattcgcgc accttgttag
    14041 gctccataga cgacgcagcc acgcgccgca tataccgctt caggctcgat ggcggtgcac
    14101 cggtggcggt tacgaaggct tcgagcttca gttcactggc gttgtcgggc aatggcaagg
    14161 cgctggtggg aattcgccag agcttcagcg aaccgcccac cctggtgtcg attgcaccgc
    14221 gcaccggcgc cgccacgaag ctttcgacat tcaacgacac ggcgctggcg agcttcgatc
    14281 agggcaaggt cgagagcgtc gtctacaagg gcgcccgcga tgcggacatc cagatgtggg
    14341 tcgtctatcc accgggcttc gactcgacga agaaatatcc ggcgatgatg ctgctgcacg
    14401 gagggccgca cagcggcatc acggatgccg tgcagtggcg ctggaacgcg caggtgttcg
    14461 caagctgggg atacgtggtg acgtggcaca acttccatgg gtcgagcggc ttcggcaatg
    14521 acttcacgga ttccatcaat ccggaccaga tcaccatgcc ctacgaggac acgatcaagg
    14581 ccgcggggtg gctgatggcg aagccgttca tcgacaagga acgcatggtg gcggccggcg
    14641 gcagttatgg cggcttcctc gccgccacgt tgttaggccg ggaccatcct ttcaaggcga
    14701 tggtggcgca cgccgccgtg tacaaccggt tcacgcagat cggcgccgat tacggggccg
    14761 agaccgaacg cttcttcaac ttctgggaca agcccgaaga gtttgcgcgc tattcgccac
    14821 acagctccgc cggcaacttc aagacaccca cgctggtcat ccacaaccag aacgacctgc
    14881 gggtgccgct gaaccacggc atcgagttgt tcaataccct gcagaaacgc ggcgtaccat
    14941 cgaagctggt gtatttcccg gatgagaacc attgggtgtt gaagccgcag aattcactgt
    15001 tctggtatca gacggtgcgc gactgggttg caacttacgc caagcctggc ccgtactagt
    15061 tgtggagacg cgcatgaaac gacgcacgct gcttgccctc accaccgccg gcgccgccgc
    15121 cctcgcgctg ccgtcatggg cggccagaaa accgaagccg ctgaagatcc tcatccttgg
    15181 cggaacacgt ttcatcgggc tgcacatgac ggcgctggcg ctgaagcgtg gccatacgct
    15241 caccttcttc aatcgcggca agaccaagac ggaccggtat cccgaaatcg aacgcatcaa
    15301 gggcgatcgc aatggcgaga tcgacgggct caaggatcgg gaatgggacg tggtcatcga
    15361 caactccggc tacgtgcctc gccatgtacg gctctcggcg gagctgctgg cgcccaaggt
    15421 gaagcagtac gtgttcgtgt cgtcgatctc ggtgtacccg gacttcagcg tgccgcgcga
    15481 cgagaaatcg cccgtgggca agctcgccga tgaaaccgtc gagaaagtgg acggggaaac
    15541 ctacggaccg ctgaaggcgc tgtgcgaaca ggcggtggag aaggcgctgc ccggccgcac
    15601 gacgatcatc cggcccgggc tcatcgtcgg ccccgacgac aacaccgatc gcttcaccta
    15661 ctggcccgcg cgcgccgcgc gcggcgggga gttcatcgcg ccgggcgcgc ccagcgatgc
    15721 gttccaggtc atcgacgtgc gcgacctcgc ggcgttcacg atcaacgccg tcgagaacaa
    15781 ctttatcggc acctacaacc tcgtgtcgaa cgtcaacgag ttcaagttcg gcgagctcac
    15841 cgacgcctgc gttgcagccg cgcgcaagca ggcgaaaccc gccgatgcgc cgcgcgccac
    15901 ctatctaccg acggccttcc tcgaggaaca gaaggtcgag ccgtggagcg agatgccggt
    15961 atggttgccg gccaagggcg acgaaacggc gttcgccggc accagcaaca aggcggcgaa
    16021 ggcccgcggt ctcaagatca ccccgatcaa gaagaccgtc gatgacacgc tcgcgtggca
    16081 cctgacgcgt cccgaggaag aacgcgtcaa gctcaaggcc ggcatcgcgc ccgagaagga
    16141 agccgcagtg ctcgcggcgt ggaaggctaa gaaggcctga actcaaagcg gcccgaaggg
    16201 ctgcgcatcg gcgatgtcgt agatgtcgag ctgcttgccg gagcgcacca tgcgcttgcc
    16261 gagcttgggg aaatccgcca cttcgaaatc acggttttcg ccgcccgcca gatacaagag
    16321 gtccgcgggc ccgggattgc gcatgtgatg cgccacggaa ggcgccggaa aaccgatgaa
    16381 gtcaccggcg ttcacctcgt attccacgtc gtcgatgagc gcgatcgcct ttcccgacag
    16441 cacgtagatc cactcttctt cacactgatg tgagtggtag acgtacgact ccttgcccgc
    16501 cggtacgcgg atgaaattca cgccagtgcg cttcagtccg acgctgcgcg ccagctgcgt
    16561 accgtggagt tcggagttcg ggttccaggg atgggaaaat tcctgggagt gcgcggcgat
    16621 gtccgcggcg cgcacgatgt tgaggctggg tttgtccatg ccgcaacctt agcaatttcg
    16681 tcgggaattc accggccgga agtacagctt ccggccggtg tgtcacatca ggctggcggt
    16741 gcctctttct cggcgctgtt cttgccgaga aaccagatga ttccgccgac cacgatcagc
    16801 gcgatgcgga taccgttgcc gatgccaagg ccccagttgt cgacccagct cagcaacgtg
    16861 aactccatgt ccatgtagtg cagcacgaaa gagccggcac ccatgagtac cagaaaacca
    16921 cctaaacctt gcattgtttc tcccctcgtc gcttgtagtt gttatcgacg gaacgggaca
    16981 gtacgccccc aggctagcta gggcaagcct cgagccttca tagatagagt ctagcgagcg
    17041 tgtacgcaat catgcccagg ccgaggcatc cgagaatgtt gcgcgaccag caaagccaca
    17101 gtcccgcggc cagtgcgccg atgagccgcg gattgagaag cgacagatcg atcgcggccc
    17161 cgggcggatg gatcaggatg tccggcacga tgatggcggc gagtgtgcac accggcgcgt
    17221 aacgcagcgc aacttcgact cgcgccccaa ggcgcaactt cgcgccggca atcaggaacg
    17281 aagttcgcgt tacaaaggtg gacagtgcga ggccggcgat caccacggcg agcgcccaac
    17341 ctgtgaccgg atcgctcatg tgtcctccgc cgattgcggt ggcgagcgct gctgtgccac
    17401 atcggtgagc gtagcggcca cgatgccggc gatggcgcca caaaagaggc cgagcttgaa
    17461 cggcatcgga taggcgatga gcgcgaccac cgccgccgcc gcggctccga cgatggcggg
    17521 ccgcgcattc aggctcgggc cgacgagcgc cacgagcgcg agcgtgccgg tgaattccag
    17581 gccccagtcg gtgggcacgt aactcgcgcc gatgattccc gtaatcgccg acaactgcca
    17641 ggtgatccag ttgttggcgc agacgccggc gaaccacgcg tcgcgatgtt ccggcagcgc
    17701 gccttcctgg gctgatcgca tgaagcgggt gaacgtgaag tccgtcatga agaagcccag
    17761 cgccgcgcgc ctgcgccagg gcaggttggc gaaatgtggg cgcagggccg cgctgtagat
    17821 gacgaagcga agattggtga tcagcccggt ggcgacgatg acccagagcg gcgcgccggc
    17881 gacgatgagt ggcagcgccg cgagttgtgc cgaacctgcg tacaccagaa gcgacatcat
    17941 gatggcccag tgaacggcaa gaccgccctt gaccatggcg acaccactga ccattcccca
    18001 ggcgaacgcg ccgggcagcg aaggaagaat ggctcgttga ccgagccgaa acgccgcgcg
    18061 gcgctccagg ttctgcgcgc ttaccacacg ccggtgttgg ccatcgacac ccagggctcg
    18121 cgcggcggca gcgagccatc cttctgcagt agttcgatcg agatgttgtc aggcgagcgc
    18181 acgaatgcca tgcggccgtc gcgcggcggg cggttgatgg tcacgccgtg tttctgcaac
    18241 cgctcgcagg tggcgtagat atcgtccacc gcatacgcca ggtggccgaa attgcggccg
    18301 ccgccgtaag cttccggttc ccagttgtgc gtgagctcca cctgcgccgt gtggtcaccg
    18361 ggcgcggcca ggaagatgag cgtgaacttg ccttgcgggt agtcggtgcg cttgacgact
    18421 tccaggccga gcgcgtcgca gtagaacttc agcgaggcat cgacgtcggt gacacgcacc
    18481 atggtgtgca ggtacttcat cgccactcga ccagttagaa catctccgag gcgccgggtg
    18541 gcttcacgtc caccttcttg cgcgacggaa tccagtcgcc ggtgatcatg gcttcgtacg
    18601 gctgtcccgg gccgaccatg gggtacacgc ccgcatcggg atcgatgatg acttcgagga
    18661 aggccgcgcc cttgaacgcg aggaattctt cgatgacgcg cggaacgtcg gctttcctgt
    18721 cgagccgcac ggcgtacttg aagccgtccg cctgggccgc cttgatgaag tccttggtgt
    18781 gcagcgtctt ctcggaggcc gacagccggc ccttgaagaa cagcttctgc cactgcttca
    18841 ccatgccatc cccattgtta ttgaggatga cgaccttcac gggcaggtcg taggtggtga
    18901 cggtttcgag ctcgccgaag ttcatgcgca ccgaagcgtc gccatcgatg tcgatgacca
    18961 gtttgtcgcg tcgcgcgaac tgcgcgccga tggcggcggg caagccgaag cccatcgtgc
    19021 ccatggagcc ggaggtgagc cattggcgcg gattacggaa atcgcagtac tgcgcagccc
    19081 acatctggtg ctggccgaca cccgtggaga tgatggcctc gcctttcgtg actcggttga
    19141 gctcttcgat gacgtagtaa ggctggatct tctcgctgtc gcgatcgtag ttcatcgcgt
    19201 aggtgcgccg caggtcggcg agatacgcga gccacttcga ccggtcccgc ttgaagccgg
    19261 cgcgacggcc gtgc
//
LOCUS       DASQJS010000150         4830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4476560, whole genome shotgun sequence.
ACCESSION   DASQJS010000150 DASQJS010000000
VERSION     DASQJS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4476560"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..1158
                     /gene="cysD"
                     /locus_tag="VEW08_06185"
     CDS             193..1158
                     /gene="cysD"
                     /locus_tag="VEW08_06185"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HYJ40360.1"
                     /translation="MQPQIQSVTPLKPVPGSRARPAASKLTHLARLEAESIHIFREVA
                     AEFAKPLLMYSIGKDSSVLLHLARKAFFPGRIPFPLLHIDTGWKFKEMISFRDETARR
                     LNAELIVHTNEAGKTAGVSPFTHGARYTDIMKTEALKQALDKYGCDAAIGGARRDEEK
                     SRAKERVFSFRDAQHRWDPKNQRPELWNLYNGRLRRGDSIRVFPLSNWTEVDIWTYIH
                     TENIPVVPLYFAKPRPIVDRDGLKIMVDDDRFPLNPGEKPHLETIRFRSLGCYPLTGA
                     QVSKAANVPEIIEEMLLTTTSERQSRAIDADSAAAMENRKREGYF"
     gene            1158..3116
                     /gene="cysN"
                     /locus_tag="VEW08_06190"
     CDS             1158..3116
                     /gene="cysN"
                     /locus_tag="VEW08_06190"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HYJ40361.1"
                     /translation="MAAVPVKPAASTPVEAPRTLEQFLAQQREIDLLRFITCGSVDDG
                     KSTLIGRLLHDTRQVFDDQLSAVANDSRRWGTTGEVPDLALLVDGLQAEREQGITIDV
                     AYRYFQTPRRKFIVADCPGHEQYTRNMATGASTADAAVVLVDAEKGVRVQTRRHTHIV
                     SLLGVQNVILAVNKMDRIDYDKSKFEAIAGEFVLYARKLGVKNIEVIPVAALVGVNVV
                     SRGTKETPWYTGRTLLEALEGIEAGRNDLLTELRFPIQLVSRPDANFRGYAGTIASGE
                     IAVGDAVLALPSRKSSKVREITTADGPLEKAGTHQAVTIVLEDEIDLSRGDVLVHPDH
                     RPTLARTFDANLIWMGEAPLLPGKRYEFKIGSRYVKGLVDSIQHRIDVNDLTTRSADA
                     LDLNGLARVRIALEEPVAVDDYKLSRATGSFIIIDLLHFGTVGAGMVIAPANQAGRAN
                     SDIVWQPTRVTAAIRANQKSQKPAVVWFTGLSGSGKSTISNALEQALVQRGHHSYLLD
                     GDNIRHGLNIDLDFSENGRNENIRRVGEVSALFVDAGLIVITAFISPFRADRNRIRER
                     IGDAQFIEVYLSTSLEECERRDPKGLYVKARAGEIREFTGIDSPYEPPIAPQLTIDTS
                     KQDIGASVDQILRYLENRGFLHQTDPGL"
     gene            3127..>4830
                     /locus_tag="VEW08_06195"
     CDS             3127..>4830
                     /locus_tag="VEW08_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HYJ40362.1"
                     /translation="MSGGVVIQGNFPIPEDRRDQLQQLATELNREQLMWLSGYFAGAA
                     AAAPSSIPGFQANLLGGQSRAQLSSAAAPAAFAPAAAAAAAPLPTVTVISASHTGNGR
                     KISEKLTAAVQALGMQARMIKAGDYQPREIAKEKLLYVVISTHGDGDPPDEARGLYEF
                     LGTKRAPQLPELQYSVLALGDTSYPKFCEAGRVIDERLAKLGAKRLLPRVDCDVDFEK
                     LAQTWADDALARVREYKEKHAPVGGVATGVAQTSAPAAAIPELTRANPATAEVLANQR
                     IVGRGALREVRHVEVSFPGLGNLYKAGDALGIVHRNPEPAIDAVLKATRLDAAAAVTH
                     EGKTHTLLEWLRDERELTRIARPLLVQVAKHANVDISELLGNAQAMAKLTATCQVSDI
                     LGTYPAEWTPEALVNVLRPVTGRVYSIASSPAVVGDEAHLTVAVVGSDTDRKLLAGAA
                     SHFVVNTPADSKVQVWIEKNERFRVPADSSRDMIMVGPGTGIAPFRGFVQEREATGAT
                     GRNWVFFGGRSLYHDFLYQLEWQQALKRKSVHRVDVAFSRDQAEKIYVQHRIRQAGKD
                     LF"
BASE COUNT          905 a         1640 c         1482 g          803 t
ORIGIN      
        1 ggggacgcgc cagggggcgc gtccccgccc cctgattact tgcacccagg ctcccgaaca
       61 cccctgtaac cgccctgtta ttcctgcata accagaaatg ctttccgggc agggggttgc
      121 gacctataaa gtcccccgca ctttggtaag ccggccctct ggccggctgc tgctattgcc
      181 ggaaagggat cgatgcaacc gcaaattcaa tcagttacgc cgctgaaacc cgttcctggg
      241 agccgtgctc gtccggccgc gtccaagctg acccacctgg cccggctcga ggccgaaagc
      301 atccacatct tccgcgaagt ggcggccgag ttcgccaagc cgctgctcat gtattccatc
      361 ggcaaggact cctcggtcct gctgcacctc gcccgcaagg cctttttccc gggccgtatt
      421 cctttcccgc tgctgcacat cgacacgggc tggaagttca aggagatgat ttcgttccgc
      481 gacgagaccg ccaggcggct gaacgccgag ctcatcgtcc acacgaatga agccggcaag
      541 accgccggcg tttcgccgtt cacgcacggt gcgcgttaca ccgacatcat gaagaccgag
      601 gcgctcaagc aggcgctcga caaatacggc tgcgacgccg ccatcggtgg cgctcgccgc
      661 gacgaagaga agtcccgcgc caaggagcgc gtgttcagct tccgcgatgc gcagcatcgc
      721 tgggacccga agaaccagcg ccccgaactc tggaacctct acaacggccg gctgcgccgc
      781 ggcgacagca tccgcgtgtt cccattgtcg aactggaccg aggtcgacat ctggacctac
      841 atccacaccg aaaacatccc ggtggtaccg ctgtatttcg ccaagccgcg tccgatcgtc
      901 gaccgcgacg ggctcaagat catggtcgac gacgaccgct tcccgctcaa cccgggtgag
      961 aagccgcacc tggagaccat tcgcttccgt tcgctcggct gctatccact gaccggcgcg
     1021 caagtgtcca aggccgcgaa cgtcccggag atcatcgagg agatgctgct caccacgacg
     1081 tccgagcgcc agagccgcgc catcgacgct gactccgcgg ccgccatgga aaatcgcaag
     1141 cgcgaagggt atttctgatg gccgccgtcc cagtcaaacc ggctgccagc actccggtgg
     1201 aggcacctcg caccctggaa caattcctcg cgcaacagcg cgagatcgac ttgctgcgtt
     1261 tcatcacctg cggatccgtc gatgacggca agagcacgct gatcggccgc ctgctgcacg
     1321 acacgcgcca ggtattcgac gaccagcttt cggccgtggc gaatgacagc cgccgctggg
     1381 gcaccaccgg cgaagtgccg gacctcgctt tgctggtcga cggcctccag gccgagcgtg
     1441 agcagggcat cacgattgac gtcgcatacc gctacttcca gacgcccagg cgcaagttca
     1501 tcgtcgccga ctgccccggc cacgagcagt acacgcgcaa catggccacc ggcgcctcga
     1561 ccgccgatgc cgccgtggtg ctcgtcgacg cggagaaggg cgtgcgcgtg cagacgcgcc
     1621 gccacacgca catcgtctcc ttgttaggcg tgcagaacgt gattctcgcc gtcaacaaga
     1681 tggaccgaat cgactacgac aagtcgaagt tcgaagcgat cgccggcgag ttcgtgctct
     1741 acgcgcgcaa gctcggcgtg aagaacatcg aggtgattcc ggttgcagcg ctggtcggtg
     1801 tgaacgtcgt ttcgcgcggc acgaaggaaa cgccctggta caccgggcgg acgctgctcg
     1861 aggctctcga aggcatcgag gcgggccgca acgacctgct cacggaactg cgtttcccga
     1921 tccagctagt gagccgcccc gatgccaact tccgcggtta cgcgggcacc atcgcgagcg
     1981 gcgaaattgc cgtgggcgac gcggtgctcg cattgccctc gcgcaagtcg agcaaggtgc
     2041 gcgagatcac cacggccgat ggcccgctcg agaaggccgg cacgcaccag gccgtcacca
     2101 ttgtcctcga ggatgaaatc gacctgtcgc gcggcgacgt gctcgtgcat ccggatcatc
     2161 gcccgacact ggcgcgcacg ttcgatgcga acctcatctg gatgggtgag gcgccgctgc
     2221 tgccgggcaa gcgttacgaa ttcaagatcg gcagtcgcta cgtcaaaggc ctggtcgatt
     2281 cgatccagca ccgcatcgac gtgaacgatc tgacgacccg cagcgccgat gcgctggatc
     2341 tcaacggcct tgcgcgcgtg cgcatcgcgt tggaagagcc cgtggctgtc gatgactaca
     2401 agctgagtcg cgccaccggc tctttcatca tcatcgacct gctgcacttc ggcaccgtgg
     2461 gtgccggcat ggtcattgcg cctgccaacc aggcaggccg cgcgaactcc gacatcgtgt
     2521 ggcaacccac acgcgtcacg gccgcgatcc gtgccaacca gaagagccag aaacccgccg
     2581 tggtatggtt caccggtctg tccggctcag gcaagtccac gatctccaat gcgctcgaac
     2641 aggctctggt gcaacgcggc catcacagct acctgctgga cggcgacaac atccgccatg
     2701 gcctcaacat cgatctcgat ttctccgaaa acgggcgcaa cgagaacatc cgccgcgtcg
     2761 gtgaggtgtc ggcgctgttc gtggatgccg ggctcatcgt catcaccgcc ttcatctcgc
     2821 cgttccgtgc ggatcgcaac aggatccgtg agcgcatcgg cgatgcgcaa ttcatcgagg
     2881 tctatctgtc cacttcactc gaagagtgcg agcgccgcga tcccaagggc ctctacgtga
     2941 aagcgcgtgc cggcgagatc cgcgagttca ccggcatcga ttcgccatat gagccgccga
     3001 tcgcgccgca attgaccatc gacacttcga agcaggacat cggtgcatcg gtggaccaga
     3061 tcctccgcta tctcgaaaac cggggcttcc tgcaccagac ggaccctggt ctatgaagag
     3121 gggactatga gcggcggcgt cgtcatccag ggaaatttcc cgattccgga ggatcgccgg
     3181 gatcaactgc agcagctcgc caccgagttg aatcgcgagc agctgatgtg gctgtcgggt
     3241 tacttcgccg gcgctgccgc ggccgcgccg tccagcattc cagggttcca ggccaatctg
     3301 ctcggcggtc agtcacgcgc gcaactgtcg tccgccgccg cgcccgctgc gtttgcaccg
     3361 gcggctgccg ccgctgccgc gccgctgccc acggtgaccg tcatctccgc ttcgcacacc
     3421 ggtaacggcc gcaagatttc ggagaagctg acggccgcgg tccaggcgct cggcatgcag
     3481 gcgcgcatga tcaaggccgg cgactaccag ccgcgcgaga tcgccaaaga gaaactgctc
     3541 tacgtggtca tcagcacgca cggtgatggc gacccgccgg atgaggcgcg cggactctac
     3601 gaattcctcg gcaccaagcg ggcgccgcaa ctacccgagc tgcagtactc ggtgctcgcg
     3661 ttaggcgaca ccagctatcc caagttctgc gaagccggac gcgtcatcga tgaacgtctc
     3721 gccaagcttg gtgccaagcg cctgctgccg cgcgtcgatt gcgacgtgga cttcgagaag
     3781 ctggcgcaga cctgggctga tgacgcgctc gcgcgcgtgc gcgaatacaa ggaaaagcat
     3841 gcgccggtgg gcggcgttgc taccggtgtc gctcaaacct ccgcgccggc cgccgccatt
     3901 cccgaactca cgcgcgcgaa ccccgcgact gccgaggtgc tggccaacca gcgcatcgtg
     3961 ggccgcggcg cgctgcgcga agtgcgccac gtggaagtgt cgtttccggg actcggcaat
     4021 ctctacaagg ccggtgatgc gctcggcatc gtgcatcgca atcccgaacc ggccatcgac
     4081 gccgtattga aagcgaccag gctcgacgcg gccgccgcgg tgacgcacga aggcaagacg
     4141 cacacgctgc tcgagtggct gcgcgacgag cgtgagctca cgcgcatcgc gcgtccgctg
     4201 ctggtgcagg tggccaagca cgcaaacgtc gatatttccg agttgctcgg caacgcgcag
     4261 gcgatggcga aactcaccgc cacttgccag gtttccgaca tcctgggcac ttacccggcg
     4321 gaatggactc ccgaggcgct ggtgaatgtg ctgcgtccgg tcacaggtcg cgtgtactcg
     4381 atcgcctcca gccccgcggt cgtcggcgac gaggcgcatc tgacagtcgc cgtggtgggc
     4441 agcgataccg atcgcaaact actcgccggt gccgcctcgc atttcgtggt caacacaccg
     4501 gccgacagca aggtccaggt gtggatcgag aagaacgaac gcttccgtgt gccggccgac
     4561 agctcgcgcg acatgatcat ggtggggccc ggcaccggca tcgcgccgtt ccgcgggttc
     4621 gtacaggaac gcgaagccac gggcgcgacg gggcgcaact gggtgttctt cggcggccgc
     4681 tcgctgtatc acgacttcct ctaccagctc gagtggcagc aggcgctcaa gcgcaagtcc
     4741 gtgcatcgcg tggacgtggc gttctcgcgc gaccaggccg aaaaaatcta cgtgcagcac
     4801 cgcatcaggc aggccggcaa agatctcttt
//
LOCUS       DASQJS010000151         5171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1022583, whole genome shotgun sequence.
ACCESSION   DASQJS010000151 DASQJS010000000
VERSION     DASQJS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022583"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /gene="purH"
                     /locus_tag="VEW08_06200"
     CDS             <1..1051
                     /gene="purH"
                     /locus_tag="VEW08_06200"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HYJ40363.1"
                     /translation="KNHESVTVVVDPADYGQVLGELDANDGATSIDTRSSLAAKAFAH
                     TAKYDTMVSNYLLGRESDDAASFPATLPLVYEKIQDLRYGENPHQKAAFYRELGLTGA
                     CVAGATMLQGKELSFNNIADTDTAVECVRVFNEPACVIVKHANPCGVATGVSLADAYD
                     RAYRTDPTSAFGGIIAFNRELDAETAIAISGRQFVEVIAAPSVSAEAAAVLAGKANVR
                     VLVLGPLASKTPTELEYRSVVGGLLVQSRDTALAQPDTFKIVTEKRPTPTQYADLWFA
                     WRVCKYVKSNAIVFARDGMTIGVGAGQMSRVYSTHVAALKAKDEGLEVEGSSMASDAF
                     FPFRDGIDAAAKYGI"
     gene            1162..2460
                     /gene="purD"
                     /locus_tag="VEW08_06205"
     CDS             1162..2460
                     /gene="purD"
                     /locus_tag="VEW08_06205"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HYJ40364.1"
                     /translation="MKILIVGSGGREHALAWKCAQSPRVSEVLVAPGNGGTATEPRVR
                     NVNVAAEDIAGLVALAKAERVGLTIIGPEGPLVIGVVDAFAAAGLRCFGPRKAAAQLE
                     GSKAYTKEFLKRHGIPTAAYATFTRETFDPAWVRAQRTPIVVKASGLAAGKGVVIVDT
                     AEEAIATAQSMFGGQFGAAGDQVVIEEFLPGEEASFIVMADGKNIVTMATSQDHKRLR
                     DADAGPNTGGMGAYSPAPVVDRAMHERIMREVIEPSIRGLAKDGTPYTGFLYAGIMVH
                     PDGTPNVLEYNCRFGDPETQPIMMRLKSDLVELCEAALDHRLDSVHADWDSRAALGVV
                     LAAEGYPDSVRKGDEIAGLGAAAKLPGKVFHAGTKAEGGKVLTHGGRVLCAVGLGDSV
                     SAAQSQAYGLVDAISWQGMQCRRDIGYRAIERERAKPTRS"
     gene            2550..4175
                     /locus_tag="VEW08_06210"
     CDS             2550..4175
                     /locus_tag="VEW08_06210"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HYJ40365.1"
                     /translation="MQKSTEQEWQFTAPGLDVVRAWLAAQPHEITDRRLAIQPTLELT
                     DIYFDSPDWMIFRAGFALRMRSERTGADVEQTEVTLKSLSAPHNGLARRTEFSQLVDG
                     ADMNAVVANMTGIGVRIRELIGERPLTPLFKANTRRERRQLLEAGSDLALAEVDLDET
                     SIEAPSGALQQLKRVEVECINATPAALEPFVMQLRDAAQLEPVQMSKFRAGLNAAGLQ
                     PTGQLELASVEISAAQPFAETQFALLRRYFAVVLEKEPLVRSGSPAAVHEMRVAARRL
                     DVLLRAFSGYGPRWAAASRGTLRAIIKALGEVRDRDVQLEYLHNSSAALTGTDRAAIE
                     PLRERLAQQRVRARARLLHTLDSTRTRSWVAHWLDQLRLGTDSGARARAATTGSVARE
                     LIRSSAGKLRKRADRLNDNSSAKDFHGVRIRAKRLRYTLDAFGSLYGDAAREYLDALA
                     KLQHVLGEYHDSTVRAGLFAEIVTSGRRVPAATSFLVGRLVERDQGKFRECQRKFSKA
                     YRRIKRRRWRELLAAMREQERAAVKIPGGAGIT"
     gene            4175..4672
                     /locus_tag="VEW08_06215"
     CDS             4175..4672
                     /locus_tag="VEW08_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYJ40366.1"
                     /translation="MDVYLVRHAIAHERNSTRWPNDALRPLTTAGLRKFRKAAAGLAR
                     CVPKTAAVFTSPYVRARETAAVLSESAKFRAAIEATELAPGKTPRAVFDLLRTRRRAA
                     VVLVGHEPDLSAWVSAALAVEPLRVKSEFKKGGAVCVRFEKTIGPGSAMLVWMLPPRV
                     LRAMR"
     gene            complement(4683..>5171)
                     /locus_tag="VEW08_06220"
     CDS             complement(4683..>5171)
                     /locus_tag="VEW08_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40367.1"
                     /translation="TEVVASTPVTRFNVAKLPATALNRASPNADQAAMRDAVDKMDSF
                     LGIVQVHVEACAETFPDMKPRLAPLLVDWRKRHATLREKTDILYTDLIRQRFPGISND
                     GVLQFREAMRTGMRNALRKTAEALSRRNCELMPGRLTGDSYDPAKAKPEAYQLIINFP
                     MR"
BASE COUNT          941 a         1668 c         1687 g          875 t
ORIGIN      
        1 caaaaatcat gaatcggtga cggtcgtcgt cgatccggcc gactacggcc aggtgcttgg
       61 cgaactcgac gccaacgatg gcgcaaccag catcgacacg cgatcgagtc tcgccgccaa
      121 ggcgttcgcc cacaccgcga aatacgacac catggtgtcc aactatctgc tgggccgcga
      181 gagcgacgac gccgcatcgt ttccggcaac cctgccgctg gtgtacgaga aaattcagga
      241 cctgcgttat ggcgagaacc cgcaccagaa agcggcgttc tatcgcgagc tcgggctcac
      301 cggtgcgtgc gtggctggcg ccaccatgct ccagggcaag gagctttcgt tcaacaacat
      361 cgccgacacg gacaccgccg tggaatgcgt ccgcgtgttc aatgaacccg cctgcgtcat
      421 cgtcaagcac gccaacccct gcggggtcgc caccggcgtt tcgttggccg acgcgtacga
      481 ccgcgcctac cgcaccgatc ccacgtccgc attcggcgga atcatcgcgt tcaatcgcga
      541 gctcgacgct gaaacggcca ttgccatcag cggccgccag ttcgtggaag tcatcgccgc
      601 gccctcggta tccgcggaag ccgcagcagt gctcgctggt aaggcgaatg tccgcgtgct
      661 ggtgctcggc cctctcgcca gcaagacgcc gaccgagctc gaatatcgca gcgtggtggg
      721 gggtctcctg gtgcagagcc gggatacggc gctcgcgcag cccgacacct tcaagatcgt
      781 cacggaaaag cggccgacgc caacccaata cgcggacctg tggttcgcct ggcgcgtgtg
      841 caagtacgtg aaatcgaatg ccattgtgtt tgcgcgcgac ggcatgacga taggtgtcgg
      901 cgccgggcag atgtcacgcg tgtactccac gcacgtggcg gcgctcaagg cgaaggacga
      961 agggctggaa gtggaaggca gctccatggc ctcggatgcc ttctttccgt ttcgggacgg
     1021 catcgatgcc gccgccaaat acggcatcta ggccatcgtt caacccggtg gcagcaagcg
     1081 cgacgatgaa gtcatcgccg cggccaatga acacggcatg accatggtgt tcacgggcat
     1141 gcgccacttc cggcactagc catgaagatc ctgatcgtgg gttccggcgg gcgcgaacat
     1201 gcgctcgcgt ggaagtgcgc gcagtcgccc agggtttcgg aggtgctggt ggcgcccggc
     1261 aacggcggca cagccacgga gccacgcgtg cgcaacgtca acgtcgccgc cgaagacatc
     1321 gccggcctgg tggcgctcgc caaggccgaa agggtcgggc tcaccatcat cgggcccgag
     1381 ggtccgctgg tcattggcgt ggtcgacgcc ttcgctgccg caggcctgcg ctgcttcgga
     1441 ccgcgcaagg cggccgcgca gctcgaaggc tccaaggcgt acaccaagga attcctcaag
     1501 cgccacggca ttccgaccgc ggcctatgcc acgttcactc gtgaaacctt cgaccccgcc
     1561 tgggttcgcg cgcagcgcac accgatcgtg gtgaaagcca gtggacttgc cgcgggcaag
     1621 ggtgtggtca tcgtcgacac ggcggaagaa gccatcgcaa ctgcgcaatc gatgttcggc
     1681 ggccagtttg gcgcggccgg cgaccaggtg gtcatcgaag aattcctgcc cggcgaggaa
     1741 gccagcttca tcgtcatggc cgacggcaag aacatcgtga ccatggcgac gtcgcaggat
     1801 cacaagcggc tgcgcgatgc ggatgccggt cccaacactg gcggcatggg cgcgtattca
     1861 cccgcgccgg tcgtggatcg cgccatgcac gaacgcatca tgcgcgaggt gatcgagcct
     1921 tccattcgcg ggcttgcgaa ggacggcacg ccgtacacgg gctttctgta cgccggcatc
     1981 atggtgcatc ccgacggcac tcccaatgtg ctcgagtaca actgccgctt cggtgatccg
     2041 gagacgcagc ccatcatgat gcggctgaag agcgacctgg tggaactgtg cgaagccgcg
     2101 ctggatcacc ggctggactc tgtccatgcc gactgggact cgcgcgccgc tctcggcgtg
     2161 gtgctggcgg ccgaaggtta tccggacagt gtgcgcaagg gcgatgagat cgcggggctt
     2221 ggcgccgcgg ccaaactacc cggcaaggta ttccatgccg gcacgaaggc cgagggcggc
     2281 aaggtgctca cgcacggcgg gcgcgtgttg tgcgcggtcg gcctgggcga cagcgtctcg
     2341 gcggcgcaaa gccaggctta cggtctggtg gacgccatca gctggcaagg catgcagtgc
     2401 cggcgggata tcggctatcg cgccatcgaa agagagcgag cgaagccaac ccgctcctga
     2461 actgagccca ttgcggtggt ggactcgatc cgcgtgccgg gcctacagtt ccggcgggaa
     2521 ttcgcgcatg gcgcgatggt cagaaaaaca tgcaaaagag caccgagcag gaatggcagt
     2581 tcaccgctcc cggcctcgac gtcgtgcgtg cctggcttgc agcgcaaccc catgaaatca
     2641 ctgatcgccg gctggccatt cagccgacgc tcgaactcac cgacatttac ttcgattcgc
     2701 ccgactggat gatcttccgc gccggctttg cgctgcgcat gcgcagcgaa cgcaccggcg
     2761 ccgacgttga acaaaccgag gtcacgctga agtcgttgag cgccccgcac aacggcctgg
     2821 cgcgtcgcac ggaattttcg caactcgtcg atggcgcgga tatgaacgcc gtggtcgcca
     2881 acatgaccgg catcggcgtg cgaatccggg aactcatcgg cgaacgtccg ttgacgccgc
     2941 tgttcaaggc caacacacgt cgcgagcgca ggcagttgct cgaagcgggt tcggacctgg
     3001 cgctcgcgga agtggacctc gacgagacat ccatcgaagc tccttccggc gctttgcaac
     3061 aactgaaacg agtcgaggtc gagtgcatca acgcgacacc cgcggctctc gaacctttcg
     3121 tgatgcaact gcgcgacgcc gcccagctcg agcccgtgca gatgtccaag tttcgcgcgg
     3181 gcctgaatgc agccggcctg cagcccaccg ggcagctcga gcttgcgagt gtcgaaatct
     3241 ccgcggcgca gccgttcgcg gagactcagt ttgcattgct gcgccgttac ttcgcagtgg
     3301 tcctggaaaa ggagccgctg gtacggtccg gttccccggc cgcggtgcac gagatgcggg
     3361 tagccgcgcg ccgtctcgac gtcctgctgc gtgcgttttc ggggtacggc ccacgctggg
     3421 cggcggcgtc acgtggcacg ctgcgcgcaa tcatcaaggc gctgggcgaa gtgcgcgacc
     3481 gcgacgtgca gctcgagtat ctgcacaact cgtccgccgc gctcaccgga actgatcgcg
     3541 cggcgatcga acccttgcgc gagcgcctgg cgcaacagcg tgtccgggcg cgggcgcgac
     3601 tgctgcacac gctcgattcg acccggaccc gttcctgggt cgctcactgg ctggatcagc
     3661 tgcgcctcgg caccgacagc ggcgcccgcg cacgtgcggc gaccaccggc agtgtcgcgc
     3721 gcgagctcat ccgttcgtcg gcgggcaagc tgcgtaaacg tgccgaccgc ctcaacgaca
     3781 actcgagcgc caaagacttc cacggagtgc gcattcgcgc caagcgcttg cgctacacgc
     3841 tcgacgcgtt cggcagcctg tatggcgacg ccgcgcgcga atacctggac gcgctagcaa
     3901 aactgcaaca tgtactcggc gaatatcacg actcgacggt gcgtgccgga ttgtttgcgg
     3961 aaattgtcac gagcgggagg cgcgtgccgg cagccacatc gttcctcgta gggcgtctgg
     4021 tggagcggga tcagggcaaa ttcagggaat gtcagcgcaa attctcaaaa gcctaccggc
     4081 ggataaagcg ccgccgctgg cgcgagctgc tggcggcgat gcgcgaacag gaacgcgccg
     4141 cagtcaaaat tccgggtggc gctggaatca cgtgatggat gtctatctcg tcaggcatgc
     4201 cattgcgcac gagcgcaatt ccacgcgctg gccgaatgac gcattgcggc cgctgaccac
     4261 cgccggcttg cgcaaatttc gcaaggcagc ggcggggctg gctcgctgcg tgccgaaaac
     4321 cgcggccgta ttcacgagcc cctacgttcg cgcacgcgag acggccgcgg tcctttcgga
     4381 atccgccaag ttcagggctg ccatcgaagc gaccgaactc gcacccggca agacaccgcg
     4441 cgcggttttc gacctgctgc ggacgcggcg tcgtgccgca gtcgtactcg tcgggcacga
     4501 gccggatttg agtgcctggg tgtcggcggc attggccgtc gaaccgttgc gcgtcaagag
     4561 tgaattcaag aaaggtggcg cggtctgcgt acgcttcgag aaaacaatcg gacccgggtc
     4621 ggccatgctc gtctggatgc tgccgccgcg cgtgttgcgc gcgatgaggt agcagcccgg
     4681 gcctaacgca tcgggaaatt gatgatcaac tgatacgcct cgggtttggc cttggccgga
     4741 tcgtagctgt cgccggtcag cctgccgggc atcagctcgc aattgcgccg cgaaagcgcc
     4801 tcggccgttt tgcgcagcgc attgcgcatt ccggtgcgca tggcctcccg aaattgcagt
     4861 accccatcgt ttgaaattcc cggaaatcgc tgccggatca ggtccgtata caggatgtcg
     4921 gttttttccc gcagcgtggc gtggcgcttg cgccagtcca ccaggagcgg ggccagcctg
     4981 ggcttcatat ccggaaacgt ctctgcgcag gcctcgacgt gcacctgcac gatcccgagg
     5041 aatgaatcca tcttgtcgac ggcgtcgcgc atcgcggcct ggtcggcgtt cggtgaagcg
     5101 cgatttaatg ccgtggccgg cagcttcgcg acgttgaacc gggtgacggg tgtgctcgcg
     5161 accacctccg t
//
LOCUS       DASQJS010000152         5317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1023295, whole genome shotgun sequence.
ACCESSION   DASQJS010000152 DASQJS010000000
VERSION     DASQJS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023295"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /gene="fmt"
                     /locus_tag="VEW08_06225"
     CDS             complement(<1..713)
                     /gene="fmt"
                     /locus_tag="VEW08_06225"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010117311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HYJ40368.1"
                     /translation="MTSLRIAFAGTPQFALPALRALLKSRHQVVGVLTQPDRPAGRGQ
                     HLRASPVKLLAAEHRLPIAQPSTLKTHEGRAELSQWAPDVLVVVAYGLILPQAALDLP
                     RLGCLNIHGSLLPRWRGAAPIQRAILAGDAETGVTIMQLDAGLDTGPMLLARRHPIGS
                     HDTAGDLHDVLAELGAAALMEALDGLAAGTLAATPQPAAGATYAPKLEKSEARIDWNL
                     PAPEVDRRVRAFNPWPVAET"
     gene            complement(746..1285)
                     /gene="def"
                     /locus_tag="VEW08_06230"
     CDS             complement(746..1285)
                     /gene="def"
                     /locus_tag="VEW08_06230"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009682343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HYJ40369.1"
                     /translation="MARLQILEYPDPRLRTKAKPVEVFDAALARLADDMLETMYAAPG
                     VGLAATQVDVHKRLIVMDISEGKASPQVFCNPEILTREGVGITEEGCLSVPGIFDEVK
                     RAATIRARAQDVKGKVFEVDLDGLAAVCLQHEMDHLEGKLFVDYLSDLKRERIRKKLE
                     KDRKERAGKAREPASARSY"
     gene            1502..2710
                     /locus_tag="VEW08_06235"
     CDS             1502..2710
                     /locus_tag="VEW08_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HYJ40370.1"
                     /translation="MLAILNPRVIALMVFLAAALIGAGCKSAPPPVAQPISTLPPGVA
                     EGPAPTADDMTATEAAVAAMNEAPVAETATPTAQLIRPDAPMNYTVKRGDTMWDISAV
                     FLKDPWFWPEIWQINPQVENPHLIYPGDVLSLAYGANGDATVSISQYSGARLQPRLRS
                     EGLEGPIAEIPFSAIAAFLTRPSILTRDQAVAAPHILAFRDRHIIGGTGHEFYVKDLN
                     ATLDQRFTVMHVGEPIRDIGSNEILAYQANYVAVAVVKAPGEVSKAVLTEGAREALPG
                     DRLITEEGDRSLAFRPHAPQTAVDGQIIGLADGATQMGPYQVVVLNRGANDGIAQGTV
                     LAVDQQGEVVLDRNRDYPWSVEGFANEVQLPYERAGTLIVFKVYEKLSYGLIIGARAP
                     IRVADRVYNP"
     gene            2780..3907
                     /gene="dprA"
                     /locus_tag="VEW08_06240"
     CDS             2780..3907
                     /gene="dprA"
                     /locus_tag="VEW08_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HYJ40371.1"
                     /translation="MNSIPAATLRRVAIVARAPSLNAAQLAQLAAVEPDLAMLEWRDL
                     RAFAQLGLTTHTAAWLATPDEALIDSDLRWLESSGTGLLPATSADYPSLLRESPDAPA
                     VLFVRGDVRILGDPQLAMVGSRNPTAGGRATAREFASFFARAGLTVTSGLALGIDAAC
                     HEGALAAGGPTVAVLGCGLDQIYPRNHEDLAERIAASGALISEFPPGTEPMPACFPQR
                     NRIIAGLSHGTLVIEAAQRSGSLITARLAGVAGREVFAIPGSIHNPLARGCHELIRQG
                     AKLVERPEDVLCELKISLAAQLVASAPDEPSLPVEGVPTLDKEYKILLDALAFEPASV
                     DSLIERTGMNSESIASMLLILELDGHVAPHPGGRYSRMAGQ"
     gene            3904..4404
                     /locus_tag="VEW08_06245"
     CDS             3904..4404
                     /locus_tag="VEW08_06245"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HYJ40372.1"
                     /translation="MKPTTTGDSMTGSVLDILIYVFDQYMLAEAPEVPERETLAADLE
                     RAGFGRHNVERALDWLADLAGERSRPDIDSTQPSLRVFSADELERISTSCRGYLLQLE
                     QDGILSPLQREIVIDRLVALDGDDVDIDRLKWLVLMVLSSQPGQEQACARMEDLLFDE
                     TDSLPN"
     gene            4404..>5317
                     /locus_tag="VEW08_06250"
     CDS             4404..>5317
                     /locus_tag="VEW08_06250"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HYJ40373.1"
                     /translation="MAENLVIVESPAKAKTIKKYLGKDFEVLASYGHVRDLIPKEGAV
                     DPDNHFAMSYRSIERNEKHVDAISRALKKSKNLYLATDPDREGEAISWHLRELLKERG
                     ELKDKTVKRVVFYEITKGEVQRAVAEPRDLSLDLVNAQQARRALDYLVGFNLSPLLWK
                     KVRRGLSAGRVQSPALRMICEREHEISIFKPQEYWTLEAEGEHSAQSFPLKLIEFNGQ
                     KVEQFSFTNEGQARDVEKALNDTSQGFLHVLSIDRKQRRRNPSPPFTTSTLQQEAARK
                     LGYSAQRTMRLAQQLYEGVDFGEGAVGL"
BASE COUNT          982 a         1724 c         1672 g          939 t
ORIGIN      
        1 gtctcggcga ccggccaggg gttgaaggcg cgaacgcgcc gatccacctc cggcgccggc
       61 aggttccagt cgatgcgcgc ctccgatttc tcgagttttg gcgcgtaggt cgcgccggcg
      121 gcgggctgcg gtgtggcggc gagcgtgccc gcggcgagcc cgtcgagagc ctccatcagc
      181 gcggccgcac cgagctccgc gagcacgtcg tgcaaatcgc cggcggtatc gtgactgccg
      241 atgggatggc gacgcgcgag cagcatcgga cccgtgtcga gtccggcatc gagctgcatg
      301 atggtgaccc cggtttccgc gtcgcccgcg agaatggcgc gctggatcgg cgccgcgccg
      361 cgccagcgcg gcagcagcga accgtggatg ttcaggcatc ccaggcgagg aaggtcgagc
      421 gccgcttgcg gaagaatgag cccgtaggcg acgaccacga gcacgtccgg agcccattgc
      481 gagagttcgg cgcgtccctc gtgggtcttg agcgtcgaag gctgggctat cggaagccgg
      541 tgttcggcag ccagtagttt gaccggcgac gcgcgcagat gttgtccgcg tcccgcgggg
      601 cggtccggct gggtaagcac gccgacgacc tgatgcctgg acttgagcag cgcgcggaga
      661 gcgggcaacg caaactgcgg tgtgcccgcg aaggcaatac gtaacgaggt catgggcacg
      721 cccgaaggct tggggtgaat cgtcgtcaat aagaccgtgc ggatgccggt tcgcgtgcct
      781 tgccggcacg ctctttgcga tctttttcca gcttcttgcg aatgcgctcg cgcttgagat
      841 cggagagata gtcgacgaac agttttcctt cgagatggtc catctcgtgc tggaggcaca
      901 cggccgcgag cccatcgagg tcaacctcga agaccttgcc cttcacgtcc tgcgcgcgtg
      961 cacgaatggt ggcagcgcgc ttcacctcgt cgaaaatgcc gggcaccgac aggcagcctt
     1021 cctcggtaat ccccacccct tcgcgggtca gaatctccgg attgcagaac acctgcggac
     1081 tcgcctttcc ctctgaaata tccatgacga tcaatcgctt atggacatcc acctgagtag
     1141 ccgccagccc gacgccgggt gccgcataca tggtctccag catgtcgtcg gccagacgcg
     1201 cgagcgccgc gtcgaacacc tcgacaggct tggccttggt gcgaagacgg gggtccgggt
     1261 actcaagaat ttgcaggcgc gccatgatgt tccgctcgat tgctgctaat gtttaggcga
     1321 ttcgaccgtg gacaaggttt gcgtggcgcg gatgggggaa gatcccgtga tccagcgcac
     1381 aaacttgaca atagatcgta ggccctcgtg ctgcggcgga ttataacagt ggggtccgct
     1441 tgccccaccc aggattgagg agtggaggat gtctttcaat caacaactta gggagccgcg
     1501 cgtgctcgca attctgaacc cgcgggtcat cgccctcatg gtctttctcg ctgccgcgct
     1561 gatcggtgcc ggatgcaaat ccgcgcctcc gccagtggca cagcctatct cgacgctgcc
     1621 tccgggtgtc gccgaggggc ctgcaccgac tgccgacgat atgacggcca cggaagccgc
     1681 cgtggcggcc atgaacgaag cacccgttgc ggaaaccgct acgcccaccg cgcagctcat
     1741 ccgccccgat gcgcccatga actacaccgt gaagcgcggt gacacgatgt gggatatctc
     1801 cgcggtattt ctcaaggatc cctggttctg gcccgagatc tggcagatca atccgcaggt
     1861 ggaaaacccg cacctcattt atcccggcga cgtgttgtca ctggcctatg gcgccaatgg
     1921 cgatgcgacc gtgagcatct cgcagtacag cggcgcgcgc ctgcagccgc gtctgcgcag
     1981 cgaaggcctg gagggcccga tcgccgagat ccccttctcg gccatcgccg catttctcac
     2041 gcggcccagc atcctgacga gggatcaggc tgtggcagct ccgcacattc tcgctttccg
     2101 cgatcgccac ataatcggcg ggacgggaca tgaattttac gtcaaggatc tgaacgcaac
     2161 gctcgaccag cgcttcaccg tcatgcacgt gggcgaaccg atccgcgata tcggctcgaa
     2221 cgagatcctc gcctaccagg ccaactacgt cgcggtggcg gtggtgaagg ctccgggtga
     2281 agtgtccaag gcggtcctca ccgaaggcgc tcgcgaagcg ctgccaggcg atcggctcat
     2341 tacagaagaa ggcgacaggt cgctcgcatt cagaccgcat gcgccgcaga ccgcggtcga
     2401 cggccagatc atcggcctcg cggatggtgc cacacagatg gggccatacc aggtggtggt
     2461 cctgaatcgt ggcgcgaatg acgggattgc ccagggcacg gtactcgccg tcgaccagca
     2521 gggcgaagtg gtactcgaca ggaatcgcga ttatccgtgg agtgtggaag gcttcgcgaa
     2581 tgaggtacaa ctaccctacg agcgcgccgg cacgctcatc gtgttcaagg tgtacgaaaa
     2641 actcagctac ggcctgatca tcggtgcacg cgctcccatc cgcgtcgcgg atcgcgttta
     2701 caacccgtaa cgcatttctc gacatacaga cgagataggg ccagccatcg cgctggccct
     2761 ttcgttttag gctgccgcga tgaattcaat tccggcagcc acgcttcggc gcgtggccat
     2821 cgtggcgcgc gcgccctcgt tgaatgccgc gcagctggcg caactcgctg ccgttgagcc
     2881 cgatctcgcc atgctcgagt ggcgcgacct acgtgcattc gcgcagctcg ggctaactac
     2941 gcacaccgcc gcgtggctgg ccacgcccga cgaggcgctc atcgactccg acctgcgctg
     3001 gctcgaatcc tcgggcaccg ggttgttgcc ggcgacgtcc gccgactacc cgtcgttgtt
     3061 gcgcgagtcg ccggatgcgc cggccgtgct gttcgtgcgg ggtgacgtcc gcatcctggg
     3121 tgatccgcag ctggccatgg tcggcagccg aaatccgacc gcgggtggcc gcgccacggc
     3181 gcgcgagttc gcgtcgtttt tcgcgcgggc cggcctcacc gtcaccagtg gtctcgcgct
     3241 cggcatcgac gccgcctgtc acgagggcgc actcgccgcg ggcggcccca ccgttgccgt
     3301 actcggctgc gggctcgacc agatctatcc gcgcaatcac gaagacctgg cggagcgcat
     3361 cgcggccagc ggcgcgctga tctcggaatt cccgccgggc accgaaccaa tgccggcctg
     3421 ctttccgcag cgcaatcgca tcatcgcggg cttgagccac ggcaccctgg tcatcgaagc
     3481 ggcgcagcgg tcggggtcac tgatcaccgc ccggctggca ggtgtcgcgg gccgggaggt
     3541 ctttgccatc cccggttcca tccacaatcc actcgcccgg ggctgccatg agctcatccg
     3601 gcagggcgcc aagctggtcg agcgacccga ggatgttctc tgcgagctca agatttccct
     3661 tgcagcgcaa ctagttgcga gcgcccccga tgagccttct cttcctgtgg agggggttcc
     3721 aacgttggac aaggaataca aaatcctgtt agatgcgctc gccttcgagc cagcaagcgt
     3781 cgacagcctg atcgaacgca cggggatgaa cagcgaatcg atcgcttcga tgctgctgat
     3841 tctggagctc gacgggcacg tggcgccgca ccccggcggc cgttacagcc gcatggcggg
     3901 acaatgaagc caacgacaac cggcgacagc atgaccggaa gtgttttaga cattcttatt
     3961 tacgtgttcg accagtacat gctggctgaa gccccggaag tgcccgaacg cgaaaccctc
     4021 gccgccgatc tcgaacgcgc cggattcggc cgtcacaatg tcgagcgcgc cctcgactgg
     4081 ctggcggatc tcgccggcga acgttcacgg cccgacatcg attcgacgca gccttcgctg
     4141 cgcgtctttt ccgccgatga gctcgagcgt atctccacca gctgccgtgg ttacctgctc
     4201 cagctcgaac aggacggcat cctctcgccg ctgcagcgcg agatcgtcat cgaccgtctc
     4261 gtcgccctcg atggcgacga cgtggatatc gacaggctca agtggctggt gctcatggtg
     4321 ctgtcgagcc agccgggcca ggaacaggcc tgcgcgcgca tggaagacct gttgttcgat
     4381 gaaacggatt cgttgcccaa ctgatggctg aaaatctagt catcgtcgaa tcgccggcga
     4441 aagcaaaaac catcaagaag tacctcggca aggatttcga ggtgctggcc agctatggtc
     4501 acgtgcgcga cctcattccg aaggaaggcg cggtcgatcc ggacaatcac ttcgcgatga
     4561 gttatcgctc catcgagcgc aacgagaaac acgtcgacgc catctcgcgg gcgctgaaga
     4621 aatccaagaa cctctacctc gcgaccgacc cggaccgtga aggcgaagcc atttcctggc
     4681 acctgcggga actgctcaag gaacgcggcg agctcaagga caagacggtc aaacgcgtcg
     4741 tgttctacga gatcaccaag ggtgaagtgc agcgtgccgt cgccgaaccg cgcgatctgt
     4801 cgctggacct cgtgaacgcg cagcaggcgc ggcgcgcgct cgactacctg gtcggcttca
     4861 atctctcgcc gctgctgtgg aagaaggtgc gccgcggtct gtccgcaggg cgcgttcaaa
     4921 gtccggcgct gcgcatgatc tgcgagcgcg aacacgagat cagcatcttc aagccgcagg
     4981 agtactggac gctcgaagcc gaaggcgaac acagcgcaca gtcattcccg ctcaagctca
     5041 tcgaattcaa cggccagaag gtggagcagt tcagcttcac caacgagggc caggcacgtg
     5101 acgtcgaaaa ggcgctcaac gatacgtcgc agggtttcct gcacgtgttg tccatcgacc
     5161 gcaagcagcg ccgccgcaac ccgtcgccgc ctttcaccac ctccacgctg cagcaggaag
     5221 ccgcgcgcaa gctcggatat tccgcccagc gcaccatgcg cctggcccag cagctatatg
     5281 aaggtgtgga cttcggcgaa ggcgccgtcg gtctcat
//
LOCUS       DASQJS010000153         7467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3853478, whole genome shotgun sequence.
ACCESSION   DASQJS010000153 DASQJS010000000
VERSION     DASQJS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3853478"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VEW08_06255"
     CDS             complement(<1..363)
                     /locus_tag="VEW08_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40374.1"
                     /translation="MLERAVRERIESSAVRHGMVCRIGSVHVGVWPFLRLTGFDLDLG
                     HGVRLHADMIAATYPGRLRLTVQAANLVGPAGLKVSSPITAWYVRGTRGEDFQLTLIE
                     PQAGLSIRQQADSVSNGWN"
     gene            638..1039
                     /locus_tag="VEW08_06260"
     CDS             638..1039
                     /locus_tag="VEW08_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40375.1"
                     /translation="MKPEREAVRKVDSAPRAEMIERQLDDVCVLNGDHFVIEQHVDGG
                     CDFLASQVVDRIENPKRFSQHEVGNPRALGDGQFRCPRLDGVISHDQANQDIGVNRES
                     NANLSIVLTRWSKNRPADRGLNTASERDALG"
     gene            complement(1036..1827)
                     /locus_tag="VEW08_06265"
     CDS             complement(1036..1827)
                     /locus_tag="VEW08_06265"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HYJ40376.1"
                     /translation="MKTKKEIVDNWLPRYTGTAIEGFGEYILVTNFGNYVEIFARRTG
                     AEIMGRDRPMPNATADGITMINFGMGSANAATIMDLLSARMPKAVLLLGKCGGLKKKA
                     GIGDLVLPIAAIRGEGTSNDYLMPEVPALPAFSLQRAVSTVIRDLGLDYWTGTVYTTN
                     RRVWEHDDDFKEYLRRTRAMAIDMETATIFASGFANRIPTGALMLVSDQPMVPEGVKT
                     EASDRKVTDIYVQRHIEIGVEALQLIRRNGKSVRHLRFEDGPGGD"
     gene            complement(2469..2545)
                     /locus_tag="VEW08_06270"
     tRNA            complement(2469..2545)
                     /locus_tag="VEW08_06270"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2509..2511),aa:Met,seq:cat)
     gene            2820..5636
                     /locus_tag="VEW08_06275"
     CDS             2820..5636
                     /locus_tag="VEW08_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ40377.1"
                     /translation="MKRLQFAHLPLRTKLVAASALTASIALLIAALTQGVTSYFYSHS
                     EAYEHLHAVARVIAGRSTTAIQSQDFGQAEALVSALRVEPNVEEALLIDRQNRVLMHY
                     AGSKTLLIASKPGELTPVQKWQHDAIQSGTHQHRFDGLTALHLVYPITDQGKLIGHLY
                     VRANLVELQEKILVELGILLGSSGIALGLAYLLARRVQRQIASPLLNLVETMQAAERG
                     DYSRRADVTSDDEIGSLMHGYNGMLAKIEQREVELARQHELAEAQVVERTKSLAEANK
                     TLRQAMNDSVEACRIAEAASKAKSEFLARMSHEIRTPMNGVLGMTELLLDSKLDPRQR
                     RFAATIQSSADALLAIINDILDFSKIEAGKLRLEAQDLDIRQVVEEVIDLFAQRAHQK
                     GIELLLDIDPYLHRWAKGDELRIRQILMNLVSNALKFTASGHVLIRARGVTPSETHVS
                     LVIDVVDTGTGILPENQAAIFDAFVQEDGSTTRRFGGTGLGLAISRQLATLMGGDITV
                     VSQPGVGSTFSLAITLPAAPAIASDSSQGLMLAPGTRVLVVDDSAINVEILGSQLEAW
                     GFIVTTAASALEAQMHLDALQAENTPPKIIVLDWHMPEQNGVDWLLDVRRNPQWKSIP
                     AIMVSSVADDIEGGLVTQLAPMRRLSKPVRQSMLRRALHEALQATPFHDVSSAPAMLA
                     PENEPVLDHLSVLLVEDNMVNRTLALEMLQRLGCDAMHAGNGVEALDALEKQEFDVVL
                     MDCQMPVMDGFIATRKLREREVARKLEPTRVVALTANALAGDREACLAAGMNDYLAKP
                     FTLAQLRNLLLPSKVSRSAANKVTLDHSAIDAVRQLDPDGQDRLLSRLIALYRDDSSQ
                     LLADMENALKVGNTENVARAAHTLKSSSANLGATNVAAIARQIEHLARGGDITDLPAA
                     MTKLRAQRTVALSELEALDNARVA"
     gene            5638..>7467
                     /locus_tag="VEW08_06280"
     CDS             5638..>7467
                     /locus_tag="VEW08_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ40378.1"
                     /translation="MPSRTPSEPIHVLIVDDDAGSRLLMRRALERSGFTVSEAADGTA
                     AVAAVEAGECNAVLMDVEMPSMNGYEACKAIRAREGGAHLPIMMVTGRDDVASINTSY
                     QAGATDFLAKPINWNLLGHRVQYLVRGGRLVQELEESQGRQRAMLDALPDLLLVVDLY
                     DRIVDRLGAADNHPFLAGGIRHEQKLAELVSGDAARSIAHALDDCRVRNERTEVEFEC
                     IRDGVERRFETRIVPYDRGRLLLVLRDVTERHRAANRIRELAFFDPLTQLPNRQYLLQ
                     LLEEARREAATTGERFAVLRINLDQFKRINDSIGHGAGDALLQAVALRLGTFLKLESD
                     GAVTMPLARLAGDEFAVAIRHLQSDSDVQKAIINISAAFASPYLIRHRDFFVTCTIGI
                     AIFPDHGSFADELLRTAGLALHEAKTSGGNRGVIYSEHMRARSVERLELETELRRALE
                     QNELFLVYQPQIEVATGRIASVEALARWRHPTRGIVPPDEFIPVAEESGLIVQLSDQV
                     MRQALKQISEWWQAGCRDLRVAVNVSGAQFEAPGFPDWVFAHLDEAGLPGSCLELEIT
                     ESLLMADEAAAALAVRAVRELGVHVAIDDFGTGYSSLAYLKHFP"
BASE COUNT         1432 a         2438 c         2315 g         1282 t
ORIGIN      
        1 gttccacccg ttgctcaccg aatccgcctg ctggcgaata gacaggcctg cctgtggttc
       61 tatcagcgtg agttggaaat cctcgccgcg agtgcccctc acataccatg cagtgattgg
      121 cgagctgacc ttcaggcctg ctgggccgac tagattggcc gcctgaactg tgagtcgcag
      181 gcggccggga tacgtggctg cgatcatgtc ggcatgcagt cgcactccat ggccgaggtc
      241 gagatcgaag cctgtaaggc gcaagaacgg ccatacgccg acgtgaaccg agccgattcg
      301 acagaccatt ccgtggcgta cggctgagga ctcgatccgc tcgcgaaccg cgcgctcgag
      361 catgggcctt agcaacgcgg ccaggcttgc gaccagtgcg gcgacgattg ctgctgcgag
      421 ccaccttcgt ccgcgtgttc caaacgacat cgccgcagga tgccacaccc ggacagcggg
      481 ccggctggcg ggcgtccggc cacggtcggc gtcgatcagg tccgcttgag ttcgtcgtaa
      541 aaatggtgga aagggaggaa tcaatacccg cggcgcatcg cgatggtttc taaggaaatt
      601 ctcatacgtc gcttacggca ctaagcaaca cccgtgagtg aagccggaac gtgaggcagt
      661 cagaaaagtc gattccgccc cgcgcgcgga aatgatcgag cgccaactgg acgacgtctg
      721 cgtcctgaac ggtgatcact tcgtgattga gcagcatgtc gacggcggtt gcgattttct
      781 cgcgagccag gtcgtagacc gcatcgagaa cccaaagcgt ttcagccagc acgaggtggg
      841 aaatccacgc gcccttggcg acggccaatt ccgctgtccg cgcttggacg gcgtcatctc
      901 gcacgatcag gcgaaccagg atattggtgt caaccgcgag agtaatgcaa atctgtcaat
      961 cgttctgacc aggtggtcca aaaatcgacc ggcagatcgc gggctgaaca cagcaagcga
     1021 gcgcgatgca cttggctaat cgccgcccgg tccgtcctcg aacctcaggt gtctcacgct
     1081 cttgccgttc cgccgaatca attggagggc ttcgacaccg atctcaatgt gccgctgaac
     1141 gtagatgtcg gtgaccttcc gatccgaggc ctctgtcttc actccctccg gtaccatcgg
     1201 ttggtcactg acaagcatta gcgccccggt cggaatccgg ttggcgaaac ccgaggcgaa
     1261 gatcgtcgct gtctccatgt cgatcgccat tgcgcgtgtg cggcggagat actccttgaa
     1321 atcgtcgtcg tgttcccaaa cgcggcggtt ggtcgtgtag acggtgccgg tccagtagtc
     1381 gagacccaag tctcggatca ccgtggatac cgcacgttgc aaactgaacg ccggcaaggc
     1441 tggcacctcg ggcatcaggt agtcgttgct agtaccctcg ccgcgaattg cagcgatcgg
     1501 cagcaccaga tcgccgatgc ccgctttctt tttgagcccg ccacacttgc ccaagagcag
     1561 tacggctttc ggcatccggg cggacagcaa atccatgatc gttgcagcgt ttgcactccc
     1621 catgccgaaa ttgatcatcg tgattccgtc ggccgtcgcg ttcggcatcg ggcggtcgcg
     1681 gcccatgatc tcggcgccgg tacggcgcgc gaatatctcc acgtagttgc cgaaattcgt
     1741 taccaggatg tactcgccga atccctcgat agcagtaccc gtgtagcggg gcagccagtt
     1801 gtcgacaatt tccttcttgg tcttcacttt tcaaccaccg taaacgttgc cattcgccgc
     1861 gagcgtagca tcgaaccgaa atgcggcatg tggtggcttt catcatcgtc cggcctggtg
     1921 tcgaccgcgt ggacgagtac gtggcccgtg gcacgcgttt cgagctggaa ttggcggcct
     1981 cgagcgccgg gcactgttgt cggaatcaac gtggcaggag gcttggcgaa catcgatcct
     2041 tggcctgacc tgagatttcc ggacacatcg gatcgatgta gtgatcgact tggaggtgcg
     2101 agatgggcaa acggatttac agctggaatt caggcgcgaa gcggttcgaa gggcgaccga
     2161 acctggtgcg atgctctcgc aagtggcgcg ggagatcggg tcaagcagca gacgctgcat
     2221 cgctggtaga agcgcgacgg gcgtctgccg agcgagaagg aagccgacgc gaaatccggt
     2281 agttcggagc tcgggcgaat gcggcgcgag aacatcaaag atacgtgcag agcgcgacat
     2341 cctaaaaaag cgctcggcta cttcgagaag gaaccgggct gtagttcggg ttcattgcga
     2401 agcatcgagc gatctggccg acgcgatata agacgttacc gatgtgtccg gaacggacct
     2461 cgtaaaaatg gtggaaaggg agggaatcga accctcgacc ccggcattat gagtgccgtg
     2521 ctctaaccag ctgagctacc tttccacgtc ccggaaaatc gcttgggact ggcccaaggc
     2581 gaaggggccg cgattctccc ggcgccgcgt gcgggtgtca aggcaatccc cacgaaaaca
     2641 accacttagc cctccgccct gacaccggtg cccgggccgc ctgtcaaccg gggaaaagag
     2701 cgattcgcaa cgctgcgata cgtctctcaa ttcgggcccg ccggatcaag tgtgagcgga
     2761 atcaagccga tagctggctt tggggcgctc tcggtcgggc gccaggatgt ctaccgccca
     2821 tgaaaaggct gcagttcgct cacctgccgt tgcgtacgaa gctcgttgcc gccagcgcgt
     2881 tgacggcgtc gatcgccttg ctgatcgcgg cgctgacgca gggcgtcacc tcctatttct
     2941 acagccacag cgaagcttac gaacacctgc atgccgtcgc gcgcgtcatc gcgggccgca
     3001 gcaccacggc gatccagagc caggacttcg gccaggccga agcactggtc agcgcactgc
     3061 gcgtcgagcc gaacgtggaa gaggcactgc tgatcgatcg gcagaaccgc gtgctcatgc
     3121 actacgcggg cagcaagacg ctgctcatcg ccagcaaacc gggcgagctc acgccggtcc
     3181 agaagtggca gcacgacgcg atccagagtg gcacgcacca gcatcgtttc gatggtctca
     3241 ccgcgctgca cctggtctac ccgatcaccg accagggcaa gctcatcggc cacctgtacg
     3301 tgcgcgcgaa cctggtcgag ctgcaggaaa agatcctcgt cgagctcggt atcctgctcg
     3361 gcagttcggg cattgcgctc ggcctagcct atctactggc gcggcgcgtg caacggcaga
     3421 tcgcctctcc cctgctcaac ctggtggaga cgatgcaggc cgccgagcga ggcgactatt
     3481 cacggcgcgc cgacgtcacc tcggatgacg aaatcggctc gctcatgcac ggctacaacg
     3541 gcatgctggc caagatcgag caacgcgagg tcgagctcgc gcggcaacac gagctggccg
     3601 aggcccaggt ggtcgagcgc accaagagcc tggccgaggc gaacaagacc ctgcgccagg
     3661 ccatgaacga cagcgtcgag gcctgccgca tcgccgaagc cgcgagcaag gccaagagcg
     3721 aattcctggc gcgcatgtcg cacgaaatcc gcacgcccat gaacggcgtg ctcggcatga
     3781 ccgagctgct gctcgattcg aagctcgatc cgcgccagcg ccgcttcgca gcgaccatcc
     3841 agagctcggc cgatgcgctg ctcgccatca tcaacgacat tctcgacttc tcgaagatcg
     3901 aagccggcaa gctgcgcctg gaagcgcaag acctcgatat ccggcaggtc gtcgaggaag
     3961 tgatcgacct cttcgcgcag cgcgctcacc agaaaggcat cgagctgctg ctcgacatcg
     4021 acccttatct tcatcgctgg gccaagggcg acgagctgcg catccgccag atcctcatga
     4081 acctggtgtc gaacgcactc aagttcaccg ccagcggcca cgtgctgatc cgcgcgcgcg
     4141 gcgtgacgcc atccgaaaca catgtgtcac tggtcatcga cgtggtcgac acgggaaccg
     4201 gcatccttcc cgagaaccag gcggcgatct tcgatgcgtt cgtgcaggaa gacggctcga
     4261 ccacgcgtcg cttcggcggc acgggactcg ggctcgccat cagccgccag ctcgccacac
     4321 tcatgggcgg cgacatcacg gtggtcagcc agcccggcgt cggttcgacg ttctcgctgg
     4381 ccatcacgct gccggcggcg cccgccatcg cgtcggattc ttcgcaggga ctgatgctgg
     4441 cgcccggcac gcgcgtgctg gtcgtggacg acagcgccat caacgtggaa atcctgggct
     4501 cgcagctcga agcctggggc ttcatcgtga ccaccgcggc cagcgcgctc gaagcacaaa
     4561 tgcatctcga cgcactgcag gccgagaaca cgccgccgaa gatcattgtg ctcgactggc
     4621 acatgcccga acagaacggc gtggactggt tgttagacgt gcggcgcaat ccgcagtgga
     4681 aatccatccc cgccatcatg gtgagctcgg tcgcggacga tatcgaaggt ggcctggtca
     4741 cgcagctcgc gccgatgcgc cgcctctcca aacccgtgcg ccagagcatg ctgcgccggg
     4801 ccctgcacga agcgctgcag gcgacacctt tccacgacgt cagctccgct cccgccatgc
     4861 tcgcaccgga aaacgaaccc gtactcgatc atctctcggt cctgctggtc gaagacaaca
     4921 tggtcaaccg cactctcgcg ctcgagatgc tgcagcggct tggctgcgat gccatgcacg
     4981 ccggcaatgg cgtcgaggcg ctggacgcgc tcgagaaaca ggaattcgac gtggtgctca
     5041 tggattgcca gatgcccgtc atggacggct tcatcgccac gcgcaagctg cgcgaacgcg
     5101 aagtggcccg caagctcgag ccgacgcgcg tcgtggcgct gaccgccaat gcgctggccg
     5161 gcgatcgcga agcctgcctc gccgccggca tgaacgacta tctcgccaag cccttcacgc
     5221 tggcgcagct gcgcaacctc ctgctgccgt ccaaggtatc gcgctcggcg gccaacaagg
     5281 tgacgctgga ccattcggcc atcgacgccg tgcgccagct cgacccggat ggacaggacc
     5341 ggttgctgtc gcggttgatc gcgctctatc gcgacgattc ctcgcagctg ctcgccgaca
     5401 tggaaaatgc cctgaaggtc ggtaataccg agaacgtcgc gcgcgcggcg cacaccctga
     5461 agagctcgag cgccaacctc ggcgccacga acgtcgcggc gatcgcgcgt cagatcgaac
     5521 acctggcgcg aggcggtgat atcaccgacc tgcccgccgc catgaccaag ctgcgcgctc
     5581 agcgcaccgt cgccctctcc gagctcgaag cgctcgacaa cgccagggta gcctgaagtg
     5641 ccgagcagaa ctccttccga acccatccac gtactcatcg tcgacgatga tgccggctcg
     5701 cgcctgctca tgcggcgggc gctggagcgc agcggcttca ccgtctccga agcggccgac
     5761 ggcactgcgg ccgtcgcggc ggtcgaggcc ggcgagtgca acgctgtgct catggacgtc
     5821 gaaatgccgt cgatgaacgg ctacgaagcc tgcaaggcga tccgcgcgcg cgagggcggc
     5881 gcgcacctgc ccatcatgat ggtgacgggc cgcgacgacg tggcttcgat caacacttcg
     5941 taccaggccg gcgccacgga cttcctcgcc aaacctatca actggaacct gttaggtcat
     6001 cgcgtccaat acctggtgcg cggtggtcga ctggtgcagg aactcgagga aagccagggg
     6061 cgccagcgcg ccatgctcga cgccctgccc gacctgctgc tggtggtcga cctctacgac
     6121 cgaatcgtcg accggctggg cgcggccgac aatcatccat tcctcgccgg tggcatccgc
     6181 cacgagcaaa agctggcgga actcgtcagc ggcgatgcgg ctcgttccat cgcgcacgcg
     6241 ctcgatgact gccgcgtacg caacgagcgc accgaagtgg agttcgaatg tatccgcgac
     6301 ggcgtcgagc gccgtttcga aacgcgcatc gtgccctacg accgtgggcg cctgttgctg
     6361 gtgttgcgtg acgtgacgga acgccaccgc gccgcgaacc ggatccgcga gctcgcgttc
     6421 ttcgatccgc tgacgcagct gccgaaccgg cagtacctgc tgcagctgct ggaagaagcg
     6481 cgccgcgaag cggccaccac cggcgagcga ttcgcggtgt tgcgcatcaa tctcgaccag
     6541 ttcaaacgca tcaacgacag catcggccat ggcgccggtg acgcgctgct gcaggccgtg
     6601 gcactgcgcc tcggcacatt cctcaaactg gagagcgatg gcgcggtgac catgccactg
     6661 gcgcggctcg cgggcgacga attcgcggtc gcgatccggc acctgcagag cgattccgac
     6721 gtgcagaagg cgatcatcaa catcagcgcc gcattcgcct cgccgtacct catccggcac
     6781 cgcgatttct tcgtgacctg caccatcggc atcgccatct ttcccgacca cggcagtttc
     6841 gccgacgaac tgctgcgaac cgcgggcctg gcgttgcacg aagcgaagac ttcgggcggc
     6901 aatcgcggcg tcatctacag cgagcacatg cgcgcgcgtt ccgtggaacg gctggagctc
     6961 gaaaccgaac ttcggcgcgc gctcgaacag aacgagctgt tcctcgtcta tcaaccgcag
     7021 atcgaagtcg ccaccggccg catcgccagc gtcgaggccc tggcccgctg gcgccatccc
     7081 acgcgcggca tcgtgccgcc ggatgaattc atcccggtgg ccgaagagtc cggcctcatc
     7141 gtgcagctct ccgaccaggt gatgcgccag gcgttgaagc agatttccga atggtggcag
     7201 gccggatgcc gcgacctgcg cgtggccgtc aacgtttcgg gggcgcagtt cgaggcgccg
     7261 ggcttcccgg actgggtgtt cgcgcatctg gatgaagccg gattgccggg ctcgtgcctc
     7321 gaactcgaga tcaccgagag cctgctcatg gccgacgaag ccgccgcggc gctcgcggtg
     7381 cgggcggtgc gcgaactcgg cgtccacgtc gcgatcgacg acttcggcac tggttactcg
     7441 tcgctggcct atctcaaaca ctttccg
//
LOCUS       DASQJS010000154        10571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3228832, whole genome shotgun sequence.
ACCESSION   DASQJS010000154 DASQJS010000000
VERSION     DASQJS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10571
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3228832"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1297
                     /gene="coaBC"
                     /locus_tag="VEW08_06285"
     CDS             74..1297
                     /gene="coaBC"
                     /locus_tag="VEW08_06285"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HYJ40379.1"
                     /translation="MQGKRILLGVTGGIAAYKSPDLVRRLREQGAEVQVVLTAGAREF
                     VTPMTFQAVSGREVRSDLWDPEAEKAMSHIELARWADFVMIAPATADFIARLATGQAD
                     DLLTTLCLATSAPIALAPAMNRLMWANAATQANVATLKARGVHVWGPGEGDQACGEVG
                     AGRMLEPTQLAGNVVNAIAPTGPLAGKRVLLTAGPTRERIDPVRFISNRSSGKMGFAL
                     AAAAREAGADVTIVCGPVALATPAGVKRIDVESAADMLNAVMKNIDGVDIFVATAAVA
                     DYRPANPVECKIKKTSESMDLKMERTVDILATVAARALRPFVVGFAAETDSVEQYARQ
                     KLLKKNLDMIAANEVGHDKVFEKDDNALLVLWRDGRRELPHAPKVALARDLVALIAEL
                     YAERKKSPRLTSVSA"
     gene            1294..1791
                     /gene="dut"
                     /locus_tag="VEW08_06290"
     CDS             1294..1791
                     /gene="dut"
                     /locus_tag="VEW08_06290"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HYJ40380.1"
                     /translation="MNAQVSAQQSGIRADRALKIRILDARIGREFPLPAYATAGSAGL
                     DLRALLDAPLVLAAGKAELIPTGLAIHLDDPGLAAVILPRSGLGHKHGVVLGNLVGLI
                     DSDYQGQLMVSCWNRSATDFTVQPGERIAQMIIVPVVQVSLEVVEDFAASQRGAGGFG
                     HTGTR"
     gene            complement(1831..2505)
                     /locus_tag="VEW08_06295"
     CDS             complement(1831..2505)
                     /locus_tag="VEW08_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HYJ40381.1"
                     /translation="MRKVTFFLATLLSSTLLASVAQAQSTPAGQSKGTYKWTDENGVV
                     HYGDHVPAEYSQREQRLLNSQGLEVGKKQAEMNPKEAAEYSARQKEESRRKQHDMFLI
                     STYPSVKEIENVRDVRLDQINGQIIAAEAYISSLTTRVEGLKTRALIFAPYNTKPSAR
                     RMPDDLAEEMVRAMSELRTQNSALNAKKTEHQKVVTQFDADIKRFKELRTSAAARVNQ
                     ANTPKQ"
     gene            complement(2510..3202)
                     /gene="pyrE"
                     /locus_tag="VEW08_06300"
     CDS             complement(2510..3202)
                     /gene="pyrE"
                     /locus_tag="VEW08_06300"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HYJ40382.1"
                     /translation="MQEYQRTFVELAMSRTALRFGSFKLKSGRESPYFFNAGLFNDGE
                     AIAVLGQCYAAALARSQIGFDMLFGPAYKGIPLATTTAVALAEHHHRSVPWAFNRKEA
                     KDHGECGNIVGAPLKGRVIIVDDVITAGTAIRESVEIIRAAGATPVGVALALDRQERG
                     QGAQSAVQEVSEQYGLRCVSILTLAELIETFAQGPSDAVRISSEQIAALRAYQRDWGV
                     GAVSPDASTGAK"
     gene            3256..4536
                     /locus_tag="VEW08_06305"
     CDS             3256..4536
                     /locus_tag="VEW08_06305"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ40383.1"
                     /translation="MSAETTAVPAAKPRISEVLTQPRMLAILLLGAASGLPNPLSEAV
                     MQAWLTDLGLSNTKIGLLMYVALPYLLKPLWAPLLDRYTLPWLGRRRGWIVFIQLLLA
                     AAIGSLALFGGAHQITAIALVLLTVVFLSASQDIVIDAYRTDVARPAERGVAAAAANL
                     GYRAFSYGALAVALIVADNVSWRAAFLTLAVAMGLMTLATLWAPEPDDPRGKSLPTLA
                     DSVLIPLKELFGAPGTIALIVLIILYKVGDAFALKFFTAFMINVGFTKTEIGVVVKAV
                     LTTGSIVGALLGGIWMIKLGLRKAMMLFAIVQALTNLGYLLLYAMGKNYVVMVGAVAL
                     DALASGMGNIASVALMMALCDKRFSAFQYALLSMMALLPRYTLGGPAGYIADHGGWGA
                     YYWTSFGLAIPGIVLVWIMRKRIEALDAPQNGHA"
     gene            4511..4912
                     /locus_tag="VEW08_06310"
     CDS             4511..4912
                     /locus_tag="VEW08_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ40384.1"
                     /translation="MHLRTGMREIDTTRVLCSMAELEETGSRGFAVGEGDWPLRGFIV
                     KTADGIAAYVNYCPHAGHPLNFRPHKFLTPDRNLIMCASHGALFARDNGLCIAGPCPG
                     QSLTPVPVEVLSGYVLLAEGVDVERLANAPL"
     gene            complement(4955..5266)
                     /locus_tag="VEW08_06315"
     CDS             complement(4955..5266)
                     /locus_tag="VEW08_06315"
                     /inference="COORDINATES: protein motif:HMM:NF042799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6763 family protein"
                     /protein_id="HYJ40385.1"
                     /translation="MKPTIGNWYRIQGSDSFEVVAFDEDDGTIEMQYFDGTVEEMDLE
                     DWQAAKEIGSLEEIEPPEDWTGSVDVDPEEDDARGTVGYDKEDDRRQSAGKLEGLDLF
                     E"
     gene            5326..6012
                     /locus_tag="VEW08_06320"
     CDS             5326..6012
                     /locus_tag="VEW08_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HYJ40386.1"
                     /translation="MLLRKGVTYKNEYLAQSTMLEQALKFFIVFFVVVEPISLIPVFS
                     GLTEGANDGYKHKMALKSVLVASGILLVFALVGAGFLSAMGIAIDSFRIFGGLLLFLI
                     ALEMVFARESGTRTSSDEKVESKKRADISVFPLAFPFMAGPGALTTLLLWFGPIPVTE
                     HPVQFLLMFACALVVLALCLLAMWVAGPLMRVIGVTGTNVANRLFGVVLGALAVQFVV
                     DGLRASFTGL"
     gene            6023..6931
                     /locus_tag="VEW08_06325"
     CDS             6023..6931
                     /locus_tag="VEW08_06325"
                     /inference="COORDINATES: protein motif:HMM:NF022431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HYJ40387.1"
                     /translation="MRASSIACVLALLLPALAHPLWAGGCPPQGFDRAALETLKRSGF
                     ELADSARRQKLALALLPCLSDPDPAMRDGITFEAYYTWMRAKLLDVPTRTELLVQLGA
                     MLEAGQADRDGFRQPFAALVLAEVARTDRIEPWLTPAQRAAFIDSAARYVTSVRDYRG
                     FDPKEGWRHGVAHGADLLLQLALNPAVDRAGLDRLLGAVAAQVAPGGEHSYIYGESER
                     LARPVLFAAQRGLHTPDEWNAWLLGVAAPAPFDSWNAAFASSAGLARRHNVHAFIYAL
                     YANARASDDPKIQALVPGLQSVLKTL"
     gene            7064..8743
                     /locus_tag="VEW08_06330"
     CDS             7064..8743
                     /locus_tag="VEW08_06330"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HYJ40388.1"
                     /translation="MRKSLIGICFAIAMSFALVHSANAQVAPSHDPSRMIRNTDGRYW
                     IFTTGDGVFAMSSPNADFSGWRAEAPVFPVGTWPGWINNYVTGFGGTFWAPDVIKVGS
                     QYRLYYSCAGQGAPAAIGLATANNLAGPWTDQGLVVAGNNAIDPAPLVDGSNQWLTWG
                     NWQTGIDLLQLNTSSGKPLNSTRWDLVPGQVEAPYLHKDGSFYYLFFQRGLCCQGVNS
                     GYYTVMGRSTSVTGPYLDKNGVSVLTGGSGAGSIFLPNRDGRYIGPGHVGYGEGKLTY
                     HFYDGNDNGAPKLRVTTMSFSNGWPVAGGVAPPPSGQPPANGTYKLRNRASGKYLDNL
                     GSTADGANVAQWAGGSSNNQRWVLTTSGGFSRLRCVTGGKYLDSIGRTTDGSIVGQWA
                     SGSSPNQQWTVVPTGSYYKVINRANGKALDTGGQTADGTAMQMWFDNTSNNQQWTFEF
                     ISSTTSLAATATAATRNEVTATRDPGAALSAVQIRIDPMSRELSVRLAKGFAGEKRVS
                     VEGRNGLVWAEGAFNGVTHTLSTADIPAGSYTLRVTGNSGTIEERKILIRP"
     gene            complement(8802..9488)
                     /gene="trmB"
                     /locus_tag="VEW08_06335"
     CDS             complement(8802..9488)
                     /gene="trmB"
                     /locus_tag="VEW08_06335"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HYJ40389.1"
                     /translation="MNELRNRSIRSFVMRAGRMTVGQTRALEDLWPRYGVEFSPYVLA
                     LDEIFGRSAPRTLEIGFGNGEHLAALASAHPERDYFGIEVHRPGVGHLLMLAAANNLT
                     NVRASTHDAVEVLREQIAPDSVDEVLVLFPDPWHKKRHHKRRLIQPPFVELIASRLHA
                     GGVLRLATDWEEYALQMLEVLGAAKSLFTNLSPTGDWMPRPEERAPTRFEKRGARLGH
                     GVWDLAFQRT"
     gene            complement(9489..10076)
                     /locus_tag="VEW08_06340"
     CDS             complement(9489..10076)
                     /locus_tag="VEW08_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleosidase"
                     /protein_id="HYJ40390.1"
                     /translation="MQPEKLLIVMALPQESRGLLEHAGAEILYTGVGKVNAAAALARR
                     LAEMRCTGAGQPLVVNLGTAGSRTIPAHTVAACNRFAQRDMDVSGMGLAPGVTPFDET
                     PPVIEFPALFTHLPQVLCSTADSFATHRHDVAGDVVDMEAFALARVCLAENARFACVK
                     YITDGADSDSAAHWEAALDNAARAFLTVYQGLIAY"
     gene            10148..10221
                     /locus_tag="VEW08_06345"
     tRNA            10148..10221
                     /locus_tag="VEW08_06345"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10180..10182,aa:Gly,seq:ccc)
     gene            10298..>10571
                     /locus_tag="VEW08_06350"
     CDS             10298..>10571
                     /locus_tag="VEW08_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40391.1"
                     /translation="MKVTASVLVGVLSFGWSPLCAADETTNPVSEATPPAGTSKFRDP
                     DDGQIDLSRFLATPRRFMPIPLVVTEPAVGYGGGLAAMFLRPRTSAG"
BASE COUNT         1796 a         3327 c         3400 g         2048 t
ORIGIN      
        1 ataactgcag gaactattgg cgaaacgaaa ccgactggta aacctttgac gtgactgccg
       61 ataatgccgg cccatgcaag gcaaacggat acttctcggc gtcaccggcg gaatcgccgc
      121 gtacaagagc cccgacctcg tccggcgtct gcgcgaacag ggcgcggagg tgcaggttgt
      181 gctcaccgcc ggcgctcgcg agttcgtcac cccgatgacc ttccaggccg tctccggccg
      241 cgaagttcgc agcgacctgt gggatccgga agccgagaag gcgatgtcgc atatcgagct
      301 cgcgcgctgg gcggatttcg tgatgattgc gccggccacc gcggacttca ttgcccggct
      361 cgccaccggc caggcggacg atctcctaac tacattgtgt ctggcgactt cggcgccgat
      421 cgcgctggcg ccggccatga accgcctcat gtgggccaat gccgccaccc aggccaacgt
      481 cgccacgctg aaggcgcgcg gcgttcacgt ctgggggccc ggcgaaggcg accaggcctg
      541 cggcgaagtg ggagccgggc gcatgctcga gccgacacag ctcgcgggca atgtcgtcaa
      601 cgcgatcgcg cccacgggcc cactcgccgg caagcgcgtg ctgctcacgg cgggtcccac
      661 gcgcgaacgc atcgatccag tgcgcttcat cagcaatcgc agctccggaa aaatgggctt
      721 cgcgctcgcg gccgcggcac gcgaggccgg cgccgatgtc acgatcgtct gcggtcctgt
      781 cgcgctggct acgcccgccg gcgtgaaacg catcgacgtc gaaagtgccg cggacatgct
      841 caatgcggtg atgaagaaca tcgacggcgt cgacatcttc gtcgccacgg ccgctgtcgc
      901 cgactatcgg ccggcgaatc ccgttgaatg caagatcaag aagacatccg agtcgatgga
      961 tctcaagatg gagcgcaccg tcgacattct tgccaccgtc gcggcgcgtg cgctgcggcc
     1021 attcgtcgta gggttcgcgg cggagacgga ttccgtggaa caatacgcgc ggcaaaaact
     1081 cctcaagaag aacctcgaca tgatcgccgc gaatgaagtc ggacacgaca aggtgttcga
     1141 gaaggacgac aacgcattgc tggtgctctg gcgcgacgga cgtcgcgagt tgccgcacgc
     1201 acccaaggtt gcgctggcac gcgatctcgt ggcgctcatc gccgagctct acgccgagcg
     1261 caagaagagc ccgcgtctca ccagcgtgtc cgcatgaacg cgcaggtttc cgcccagcag
     1321 tcgggcattc gcgcggatcg cgcgctcaag atcaggattc tcgatgcgcg catcggcagg
     1381 gaatttcctt tgcctgccta cgccaccgcg ggttccgcgg ggcttgacct gcgcgctttg
     1441 ctcgatgccc cgctggtgct ggccgctggc aaggcggaac tgattcccac cggtctggcc
     1501 attcatctgg atgacccggg cctcgccgcc gtgatccttc cgcgctcggg tttgggccac
     1561 aaacacggcg tggtgctggg caacctggtg ggcctcatcg attccgacta ccaggggcaa
     1621 ctcatggtgt cgtgctggaa tcgatccgca acagacttca cggtgcagcc gggcgagcga
     1681 atcgcccaga tgatcatcgt gcccgtggtg caggtctcgt tggaggtcgt ggaggatttt
     1741 gccgcgagcc agcgcggcgc cggcggcttc ggccacactg gcacgcgctg aagcggctgc
     1801 gaagcccgtg ctttttcgcg agggcccgaa ctactgcttc ggggtgttgg cctggttaac
     1861 tcgcgcggcc gccgacgtgc gcagctcctt gaaccgcttg atgtcggcgt cgaactgcgt
     1921 caccactttc tgatgctcgg tcttcttggc gttcagcgct gaattctgcg tgcgcagctc
     1981 gctcatggcg cgaaccattt cctcggcgag atcgtcgggc atgcggcgcg cactgggctt
     2041 cgtgttgtag ggcgcgaaga tgagcgcgcg agttttcaat ccctcgacgc gagtggtgag
     2101 actcgaaatg tacgcctcgg cggcgatgat ctgaccgttg atctggtcga gccgcacgtc
     2161 gcgcacgttc tcgatttcct tcaccgacgg gtaggtggag atcaggaaca tgtcgtgctg
     2221 cttgcgccgg gattcttcct tctgcctggc ggagtattcg gcagcttcct tcggattcat
     2281 ctccgcctgt ttcttgccca cctcgagacc ctgggaattc agcagcctct gttcgcgctg
     2341 cgaatactcg gctggtacat ggtcaccgta gtgcacgacc ccgttctcgt cggtccactt
     2401 gtaggtccct ttcgactgac ccgccggcgt gctttgggcc tgcgccacgg atgccagcag
     2461 ggtgctcgag agcaatgtcg ccaggaagaa cgtgactttg cgcatgcggc tattttgccc
     2521 cagtgctagc gtccggcgat accgcgccta cgccccaatc gcgctggtaa gcccgcaatg
     2581 ccgcaatctg ttcgctggaa atgcgaactg catcactggg gccctgggcg aaggtctcga
     2641 tgagctccgc cagggtgagg atgctgacgc accgcaaacc gtactgctcc gagacctctt
     2701 gcaccgccga ttgcgccccc tggccgcgct cctggcggtc cagggcgagg gccactccga
     2761 cgggtgtggc acccgccgcg cggatgattt ccacggactc gcggatggcg gtgccggcgg
     2821 tgatcacgtc atcgacgatg atcacgcgtc ccttgagcgg cgcgcccacg atgttgccgc
     2881 actcgccatg atccttggct tccttgcggt tgaatgccca cgggacgctg cggtgatggt
     2941 gttcggcgag cgccacggcg gtggtggtgg cgagcggaat gcccttgtag gccgggccga
     3001 acaacatgtc gaacccgatt tgcgaacgcg ccagcgcggc cgcgtagcac tggccgagga
     3061 ccgcgatggc ttcgccgtca ttgaacaggc cggcattgaa gaagtacgga ctctcgcggc
     3121 cggacttcag cttgaacgag ccgaagcgca gcgcggtgcg cgacattgcg agctcgacga
     3181 aggttcgttg atattcctgc acggtcagag tcttccgtat gatgcggcgg tgcgcatgat
     3241 aacggacaac cagacttgag cgcagaaacg accgccgtgc cggctgccaa gccgcgaatc
     3301 agcgaagtgc tcacgcagcc tcgcatgctc gcgatcctgt tgttaggcgc ggcgtcgggg
     3361 ctgccaaatc cattgtccga agccgttatg caggcgtggc tcaccgatct ggggttgagc
     3421 aacaccaaga tcggtttgct gatgtacgtc gcgttgccct atctgctcaa gccgctgtgg
     3481 gcgccgctgc tcgatcgcta cacgctgccg tggctcggcc ggcgccgtgg gtggatcgta
     3541 ttcatccagc ttcttctcgc ggccgcgatc ggttcgctgg ctctttttgg cggtgcgcac
     3601 cagatcacgg cgatcgcgct ggtgctgctc acggtggtgt ttctgtcggc atcacaggac
     3661 atcgtcatcg atgcgtaccg caccgatgtc gcgcggccgg cggaacgcgg cgttgccgcc
     3721 gctgctgcca acctggggta ccgcgcgttt tcctacggcg ccctcgcggt ggcgctcatc
     3781 gtcgccgaca acgtgagctg gcgcgcagcc ttcctgacgc tggcagttgc gatgggattg
     3841 atgacactcg cgaccctctg ggccccggag ccggacgacc cgcgcggcaa gtcgctgccg
     3901 acgctggcgg actcggtgct gataccactt aaggagttgt tcggtgcccc gggcaccatc
     3961 gcgctcatcg tcctgatcat cctctacaag gtgggtgatg cgttcgcgct gaagtttttc
     4021 accgccttca tgatcaacgt cggtttcacc aagacggaaa tcggcgtggt ggtcaaggct
     4081 gtgctcacca ccggctcgat cgttggcgcg ctgcttggcg gcatctggat gatcaagctc
     4141 ggcctgcgca aggcgatgat gttgttcgcg atcgtgcagg cgctcaccaa cctgggctac
     4201 ctgttgctat acgcgatggg caagaactac gtggtcatgg tcggcgcggt ggcgctcgat
     4261 gcgctcgcga gcggcatggg taacatcgcg tccgtcgcac tcatgatggc gctgtgcgac
     4321 aagcgcttca gcgcattcca gtacgcgctg ctgtcgatga tggccttgct gccgcgctac
     4381 acgctcggtg ggcccgcggg ctacatcgct gatcacggag gttggggcgc gtactactgg
     4441 acgagtttcg ggctcgcgat tccgggaatc gtgcttgtct ggatcatgcg caagcgcatc
     4501 gaggcgctgg atgcacctca gaacgggcat gcgtgagatc gacacgacac gagtgctttg
     4561 ttcgatggcc gagctcgagg agaccggcag tcggggtttt gcagtaggcg agggcgactg
     4621 gccgctgcgc ggattcatcg tcaagaccgc ggatggcatt gccgcctacg tgaactactg
     4681 cccgcatgcc gggcatccgc tcaacttccg gccgcacaaa ttcctgaccc cggatcgcaa
     4741 tctcatcatg tgcgcatcgc atggcgcgct gttcgcgcgc gacaacgggt tgtgcatcgc
     4801 cgggccgtgc ccggggcaat cgctgacgcc ggtgccggtc gaggtgttga gcgggtatgt
     4861 gctgctggcc gaaggagtcg acgtcgagcg actggcgaac gcgccgctct agttagggag
     4921 gtccgactgg agagatgtgc tcgatccgaa aggcttactc gaacaggtcg agaccttcga
     4981 gcttgcctgc gctctggcgt cggtcgtctt ccttgtcgta gcccacggtg ccgcgtgcat
     5041 cgtcttcttc ggggtcgaca tcgaccgagc cggtccagtc ttcgggcggt tcgatttctt
     5101 cgagcgagcc tatttctttc gcagcctgcc agtcttcgag gtccatttct tcaaccgtgc
     5161 catcgaagta ctgcatctcg atggtaccgt cgtcttcatc gaaggcgacg acttcgaaag
     5221 agtcgcttcc ttgaatgcgg taccagttgc cgatggtggg tttcatcgaa tccctccgaa
     5281 tcgtgatgct gctatgctgc tcgcgccatt tcctgagtcg agttcatgtt gctccggaaa
     5341 ggtgtcactt acaagaacga atatcttgcg caaagcacca tgcttgaaca ggccctgaag
     5401 tttttcatcg tgttcttcgt cgtggtggaa ccgatctcgc tgatcccggt gttctccggc
     5461 ctgacggaag gcgcgaacga cggctacaaa cacaagatgg ccctgaagtc ggtgttggtt
     5521 gcaagcggaa ttctgctggt tttcgcgctg gtgggcgccg gctttttgtc ggcgatgggc
     5581 atcgccatcg actcgttcag gatttttggc ggtctgttgc ttttcttgat cgcgctcgag
     5641 atggtatttg cgcgtgaatc cgggacgcgt acgtcgagcg acgaaaaagt cgagagcaag
     5701 aagcgtgcgg acatttcggt tttccctctt gcattcccgt tcatggctgg tcctggtgcg
     5761 ctgacaaccc tgctgctctg gtttggaccg attcccgtga ccgagcatcc ggtccagttc
     5821 ctcctcatgt tcgcgtgcgc gctggtggtg ctggctttgt gcctgctcgc catgtgggtg
     5881 gcgggaccgc tgatgcgcgt catcggcgtc accggcacga acgtggcgaa tagattgttc
     5941 ggtgtggtgc tcggcgcact ggccgttcaa tttgtcgtcg atggcctacg cgccagcttc
     6001 accggacttt gaagcaggcc ccatgcgcgc ctcatccatc gcgtgtgtgc tcgccctgct
     6061 gttgcccgct ctcgcgcatc cgctgtgggc cggtgggtgt cccccgcagg gattcgatcg
     6121 ggcggcgctc gaaactctga agaggtcggg tttcgagctc gccgactcgg cgcggcgcca
     6181 gaaactcgcg ctggcgctcc tgccttgtct gtcggacccg gatccggcca tgcgcgacgg
     6241 cattaccttc gaagcctatt acacgtggat gcgcgcgaag cttctggatg tgccaacgcg
     6301 taccgaacta cttgtgcaac tgggcgccat gctcgaggcc ggtcaggccg atcgcgacgg
     6361 tttccgacag ccgtttgcgg cgctggtcct ggccgaggtc gcgcgcaccg accgcatcga
     6421 accctggctg acgcctgcgc agcgcgccgc gttcatcgac agcgctgcgc gctacgtaac
     6481 ctcggtgcgc gactatcgcg ggttcgatcc gaaggagggc tggcggcacg gcgttgcgca
     6541 tggagccgac ctgctgctgc aactcgcgct gaatcccgcc gtggaccgcg ccgggctcga
     6601 tcgcctgctg ggcgcggttg ccgcgcaggt ggcgccgggc ggcgagcact catatatcta
     6661 tggcgagtcc gagcggctgg cgcgcccggt gttgttcgcc gcccagcgcg gcctgcatac
     6721 gccggatgaa tggaacgcct ggctgctcgg agtggcggct ccggcaccgt tcgacagttg
     6781 gaacgcggcg tttgccagca gcgccggcct cgccaggcgt cacaacgtgc atgccttcat
     6841 ctacgcgcta tacgcgaatg cgcgcgcaag cgacgatccg aaaatccagg cgctggtgcc
     6901 ggggctgcag tcggtattga aaaccctcta gccttctagt tagagacaat ctcccatatc
     6961 gcaaacgaca gcgattgtta tgactttggg tatgttgggt gtgccgcgcg aaatcgcggc
     7021 gtagacttcc gcgcggcaac tcagacgcta cagggagtac gtcatgcgca agagtctcat
     7081 agggatatgt ttcgccatcg cgatgtcgtt cgcgctggtt cattccgcga acgcgcaggt
     7141 ggctccgagc cacgacccgt cgcgcatgat ccgcaacacc gacggccgct actggatctt
     7201 caccaccggc gacggcgtct tcgcgatgtc ctcgcccaac gccgacttca gcggctggcg
     7261 tgcggaggcc ccggtgtttc cggtcggcac atggccgggc tggatcaaca actacgtgac
     7321 gggtttcggc ggaaccttct gggcgcccga cgtcatcaag gtgggcagcc agtaccgtct
     7381 ctactattcc tgcgccggcc agggagcgcc ggctgccatc gggctcgcga cggctaacaa
     7441 cctcgccggg ccgtggacgg accaggggct cgtggtcgcc ggcaacaacg ccatcgatcc
     7501 tgcgcccctc gtcgacggca gcaaccagtg gctcacgtgg ggcaactggc aaaccggcat
     7561 cgatctgctg cagctgaata cgtccagcgg caaaccgctg aactcgacgc gctgggatct
     7621 cgtgccggga caggtggagg cgccctatct acacaaggat ggaagtttct actacctgtt
     7681 cttccagcgg gggctgtgct gccagggcgt gaacagcggc tactacacgg tgatgggccg
     7741 ctcgaccagc gtgacgggtc cgtacctcga caagaacggc gtcagcgtac tcacgggcgg
     7801 cagtggcgcc ggcagcattt tcctgccgaa ccgcgacggc aggtacatcg ggcccgggca
     7861 cgtgggatac ggcgaaggca agctgacgta tcacttctac gacggcaacg acaacggcgc
     7921 gccgaagttg cgcgtgacca cgatgtcgtt cagcaatgga tggccggtgg caggtggggt
     7981 cgcgccgccg ccttcgggac agccgccggc gaacggcact tacaaactgc gcaatcgcgc
     8041 gagcggcaag tacctcgaca atctcggttc aaccgccgat ggcgcaaatg tcgcgcagtg
     8101 ggccggtggg tcgagcaata accagcgatg ggtgttaacg acgtcgggag gcttctccag
     8161 gctccgctgc gttaccggcg gaaaatacct ggacagcatc gggcggacga ccgacggttc
     8221 catagtcggt caatgggcca gcggcagcag cccgaaccag caatggaccg tcgtgcccac
     8281 gggctcctat tacaaggtga tcaaccgcgc gaacggcaag gcgctcgata cgggcgggca
     8341 gaccgcggat ggaaccgcca tgcagatgtg gttcgacaac acgagcaaca atcagcagtg
     8401 gacgtttgaa ttcatcagct cgacgaccag cctggcggcc accgccaccg ctgcgacgcg
     8461 aaacgaagtg acggccacgc gcgacccagg cgcagcgctg agtgccgtgc agattcgcat
     8521 cgatccgatg agccgcgaac tcagcgtcag gctagccaag ggattcgcgg gcgagaaacg
     8581 ggtcagcgtc gaaggtcgca acggattggt gtgggccgaa ggcgccttca acggagtaac
     8641 gcacacgttg agcaccgctg acataccggc cggcagctac acgctgcgcg tgaccggaaa
     8701 ctcgggaacc atcgaggaac gcaagatcct catccgcccg tagtgggggg cagccccctt
     8761 ttcccagcaa aaggggtcgg gcctcacgag gtctgacccc ttcaggtgcg ctgaaacgcc
     8821 agatcccaaa cgccatgtcc cagccgcgcg ccacgctttt cgaaacgcgt cggcgcgcgt
     8881 tcctccggcc gcggcatcca gtcaccggtg ggcgaaagat tggtgaacag gctctttgcg
     8941 gcgccgagca cctcgagcat ctgtagcgcg tactcttccc agtcggtagc tagccgcaac
     9001 acgccaccgg cgtgcaggcg cgacgcgatc agttccacga acggtggctg gatcagccgg
     9061 cgtttgtgat gtctcttctt gtgccagggg tcagggaaca gcaccagcac ttcgtcgacg
     9121 gaatccggcg cgatctgctc gcgcagcacc tctaccgcgt cgtgtgtgct cgctcgcaca
     9181 ttcgtgaggt tgtttgccgc ggccagcatg agcaggtgac ccacgcccgg gcgatgcact
     9241 tcgatgccga agtagtcgcg ctccggatgc gcactcgcta acgcggcgag atgttcgccg
     9301 ttgccgaagc caatctcgag agtgcggggc gcggagcgac cgaagatttc gtccagcgcc
     9361 agcacgtacg gcgagaactc gacaccatat cgcggccaca gatcctcgag tgcgcgtgtc
     9421 tggccgacgg tcatgcggcc ggcgcgcatc acgaagctgc gaatggatcg gttgcgaagt
     9481 tcgttcattc agtatgcgat gagaccctgg tagacggtaa ggaaggcacg cgcggcatta
     9541 tccagtgccg cttcccagtg tgcggcggaa tcgctatcgg ctccgtcggt gatgtacttc
     9601 acgcaggcga agcgcgcgtt ctccgcgagg cacacgcgcg cgagggcgaa ggcttccatg
     9661 tcgacgacgt cgccggcgac atcgtgccta tgcgtcgcga acgaatccgc ggtcgaacac
     9721 aacacctgcg gcaaatgcgt gaacagcgcc ggaaactcga tgaccggcgg cgtctcgtcg
     9781 aagggcgtaa cgcccggcgc gagccccatt ccgctcacgt ccatgtcacg ttgcgcgaaa
     9841 cgattgcagg ccgccacggt gtgcgcggga atcgtccggc tgcccgcggt gcccaggttc
     9901 accaccaggg gctggccggc gccggtgcag cgcatctccg ccagtcgtcg ggccagcgcc
     9961 gccgcggcat tgaccttgcc gacgccggtg tacaggattt cggcgcccgc gtgttccagc
    10021 aggccgcgcg attcctgtgg caatgccatg acgatcaaca atttttcggg ttgcatgctt
    10081 ccacgtggtt gttcccccca agttcgctcg gctattctcg cccctctcgt ttcgaagacg
    10141 ggcgaacgcg ggtgtagttc aatggtagaa ctgtagcttc ccaagctact aacgtgggtt
    10201 cgattcccat cacccgctcc acctctgctt ttgcgacaac gtctgtgggt cgatgggctt
    10261 attgagcccg cactcttcag tgccgagtat gctgccgatg aaagttacgg cgtcggttct
    10321 ggtgggagtg ctctcattcg ggtggagccc gctgtgcgcc gcggacgaaa ccaccaatcc
    10381 cgtgtccgag gccacacctc ctgcgggtac atccaaattc cgcgatcccg acgacgggca
    10441 gatcgatctc agccggtttc tcgctactcc gcgtcgcttc atgcccattc cgctggtggt
    10501 caccgagccc gcggtgggtt acggcggcgg tctcgcggca atgttcctgc ggccgcgcac
    10561 gtcggccggc a
//
LOCUS       DASQJS010000155        35505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4479715, whole genome shotgun sequence.
ACCESSION   DASQJS010000155 DASQJS010000000
VERSION     DASQJS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 35505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35505
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4479715"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VEW08_06355"
     CDS             complement(<1..309)
                     /locus_tag="VEW08_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ40392.1"
                     /translation="MIAADRHLSVPGARLRYRDEGAGPAVVLVHGWTLDLDMWEPQAL
                     LAAQLRIVRFDRRGFGLSSGEASMRADVQDLHALITRLELPRPLLVGMSQGARVVLDF
                     A"
     gene            complement(403..1209)
                     /locus_tag="VEW08_06360"
     CDS             complement(403..1209)
                     /locus_tag="VEW08_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HYJ40393.1"
                     /translation="MPSKSSAPRREVMGGLAKGLSVIKAFSRDHSTLTLSEIAATARM
                     PAATARRCLLTLEELGYVTRRGRAFLLRPKVLELGAAYLESMDIEHLTLTHLEELARV
                     TGDSAALSVLDGNDIVYVARTSVRTLMRLEAHVGSRFPAHVTSMGRVLLAGVSAEHLD
                     QYFRTQKFVALTSQTVTEPKKLRQLIEECRRNGYSAVEDELAYGVVAVAVPVFDQAGR
                     VVAALNSSSHSRRASKASLVKQRLAMLRKASKEISVELARVPGIALSAQI"
     gene            1367..2173
                     /locus_tag="VEW08_06365"
     CDS             1367..2173
                     /locus_tag="VEW08_06365"
                     /inference="COORDINATES: protein motif:HMM:NF004868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HYJ40394.1"
                     /translation="MKIGTQAQRTSAISASNADAIVVRGRNLCTELVGVVTFTDHAWL
                     LVAGDLPSAGQRRILDATLVAIAEHGLVPSVAASRMTLAAAPEALQGAVAAGILGCGS
                     VILGAADAAGRLFNAIVTRASNEPLEAAARSVLGEMRAARQPVPGYGHPLHKKQDPRA
                     ARLLAVATEAGTSGKHIEAAILVERLVPQIWGKPLAMNVSAAIAAVLLDAGYPLLAIK
                     GVPMLARTASLIAHLLEEQQRPIGFVLAHEGAASIGYDGTCPDGFVPGEE"
     gene            2176..3360
                     /locus_tag="VEW08_06370"
     CDS             2176..3360
                     /locus_tag="VEW08_06370"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYJ40395.1"
                     /translation="MQPVLRGVKIVEQGTFITGPCAGMMLADLGADVIKIESPGGDPY
                     RAYQGGLYSPHFQAYNRNKRSVCCDLKQSADRELFDQLILDADVYIQNFRPGTAEKLG
                     AGAERLRELNPKLVYCSISGFGGSGPYVERPSYDSVAQALSGFLSVVVDEDRPRFLGP
                     ALADAITGIYAAFGVLGALLERAGSGRGKLVEVSMLEAMAHFAVEPFAAFFALGAVPK
                     SADRPRLAQAYILRTSDNRLIAIHLSSLEKFWLGLVKALEADELTSDARFSERLARIS
                     NYETLGAELDRRFRGRTLNDWSARLSANDVPFAPINGIDSVVEDPQVGHLGLIVPVNG
                     VQQGGRQAVRPPLQFDGERAHTVSAAPLLDQHGIAIRAAMTESRRWPAMSDDAPPRRA
                     TG"
     gene            3367..4419
                     /locus_tag="VEW08_06375"
     CDS             3367..4419
                     /locus_tag="VEW08_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HYJ40396.1"
                     /translation="MSETRGKLFPTTLVGSMPQPEWLIDRQKLAGRFPPRVRALELWR
                     IPEAYLAEAQNDATVLAIRAQEKAGLDIVTDGEIRRESYSNRFATALEGVDLDNPGTA
                     LDRSGHPNPVPRVVGPIRRKHPVQVDDLKFLRANTTRQVKVTVPGPFTMAQQAQIDAY
                     GGSREAAAMDYAKAVNAEIRDLFAAGADVVQIDEPYMQARPDEARAFGLKALNQALDG
                     VTGTTAVHICFGYAAIIHARPSAYSFLPELAGCSCRQVSVETAQSNLDCSALLPLKDK
                     KVMLGVLDLADMSVETPELVAERIRRALKYVPAENIILAPDCGMKYLPREIAVGKLKA
                     MVAGAAIMRTELSRAA"
     gene            4428..6632
                     /locus_tag="VEW08_06380"
     CDS             4428..6632
                     /locus_tag="VEW08_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ40397.1"
                     /translation="MHLPNKLSPMGLIGVAVAAALAATPAQAQTPPEDIDEVVVTARK
                     RNEASTDVPVTMSVFTREDITAAGIEKPGDFIARVPNMTLVETQNAGNAFVVVRGISQ
                     ARNSEPSVAVLVDGVLETNPAEFNQELFDIEQIEVLKGPQGALYGRNAIGGAIIIQTR
                     DPGDEFEGRVKLGVGNGSSVRAQAGVSGPIGEGSLRYRASLSYYDTDGYIPSTFLGGE
                     ADPVQDFSGRVRLSWAPSETFSGDLRVSASKLETRGFYFVIPRDDEANPFSTFTTPPD
                     ANNVTSPIQVNNAGIDDRDLFTGALRLNFELGGGTLTSVSAYNTTEETITGDAYDFRP
                     RDTSIFNVLLGTDLNQSQFLDLKSYSQEVRFTSNDEGRVRWIAGAYYVHTDRFISTGN
                     MVDTGNGVFPVYEEPRFGGNNPSATFLADSQDNDAWAVFADVTFELSDKFELDTAVRY
                     DEDKRENTTETPTAFLPDPSASEGEVRTHTWSETQPKVTLRYKPSEDVTFFGGWSRGF
                     RSGGFNQTGVGAVADDNGIAGVNDLFEAEVADTWEVGFKGEFLDGRVSTGVSLFQTKS
                     TNGYFFVFLAANSTQNLGNLDADYEGAEFDISVKVTDNFELFGSYGFTDSEITDMEDP
                     SVIGNQAPLVSEDTFNLGAQWRQPIGSALHLALRADYRHTGETWWEPYNVTSRDPIDI
                     VDMRLGLEGEKWAVTAWSTNLTDEEYNAEFSPGGFLFRALPIRYGLEFTYSF"
     gene            6649..7185
                     /locus_tag="VEW08_06385"
     CDS             6649..7185
                     /locus_tag="VEW08_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ40398.1"
                     /translation="MATKASLPSRLHHTAYVSRDLEATRKFYEDIIGLPLLATWCEKD
                     LLFGAERTYCHVFFGLADGGALAFFQFAEPADQEQFGPKMPFSPFHHIALHVDKETQA
                     GIEKRLKDAGFQEPQSFVLEHGYCRSVYVTDPNGMIVEFTHDHPDVENINRIALGRAH
                     SELKRWLAGDHTSNNTFR"
     gene            7185..8330
                     /locus_tag="VEW08_06390"
     CDS             7185..8330
                     /locus_tag="VEW08_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HYJ40399.1"
                     /translation="MRTSTDRILTTHVGSLPRSQAVTDVLFARERNDVPDTAAAAKVI
                     ADAVVEVVRKQVAAGVDVVSDGEMSKISYATYIADRFTGFDGDTPREPGQDLVEFPRL
                     LEKLAKLGSTAKYRRPRCVGEIRNKTFAPLQEDLANFGRGVKAGGPTDAFLNAASPGV
                     IALFQPNDFYKTADDYLEAVAEALRGEYEAIVAAGFLVQIDAPDLGMGRHTMYRNATV
                     AEYLARAERHVEVLNHALRNVPASSVRMHCCWGNYEGPHHHDVPMRDLLPVLLKARPQ
                     ALLFEAANPRHAHEWTVFRDTRLPDDKILIPGVLSSTTNYVEHPELVAERLLRFADIV
                     GRERVMAGSDCGFSTFAGFGAVDPDIVYLKLAAMAEGARLASQKLWS"
     gene            complement(8426..9730)
                     /locus_tag="VEW08_06395"
     CDS             complement(8426..9730)
                     /locus_tag="VEW08_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HYJ40400.1"
                     /translation="MIVAFWNWLDKCLFGEFSEGKTPGALALRLLRYPYALLRDLSRG
                     QINLYAMGLVYATLLSLVPLIAFAFAVLKAFGAHRELQPVILEFFKPMGSSAAEFTAK
                     VMEFADSVSTGLVGSVGLALLLWTLLGTIKKVEDGFNFLWRVEHARSFTRRVTEYVAL
                     LIVGPIVVVSFLGLSHNALESASVGIGRYMPFLERVVQFSKWISPYFMVAAIFTAVYM
                     FVPNTRVKWKPALIGGLTAGILWAAVGNVFTALVVYSTRLTVVYAGFAIIVAALLWTY
                     FGWLILLIGAQLSFYVQNRNYLRMGLTELRMSAVQREQLTLKMMFLIARAYHDGKTRW
                     SVDTLAHELAMPGIAISRIVHGLEDAHLLTMTDDEKLLPARDLGQISIQEIVDIARNE
                     KAGQVGWRNLKIPAVDAISKTMDDAWRKSCGEMTLRDLIEES"
     gene            9786..10622
                     /locus_tag="VEW08_06400"
     CDS             9786..10622
                     /locus_tag="VEW08_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HYJ40401.1"
                     /translation="MGSTYPTRIVCLTEETTETLYLLGEEARIVGISGFTVRPPRARR
                     EKPRVSAFTSAKIDRIVELKPDLVLGFSDLQADIAAELIRLGIEVHVFNQRSVAEILR
                     MIGMLGGMVGCEAKARTLIEELEAGIEAVRASAAKFARRPRVYFEEWDEPQISGIAWV
                     SELVGIAGGDDVFPELARESLGKNRIIADPLEVPRRAPEVIFGSWCGKKFQPASVAER
                     AGWSDVPAVRDGHLYEVKSSIILQPGPAALTDGLAELHRRLASWNGGAVPGRETGKTP
                     IN"
     gene            complement(10665..11576)
                     /locus_tag="VEW08_06405"
     CDS             complement(10665..11576)
                     /locus_tag="VEW08_06405"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HYJ40402.1"
                     /translation="MPTMLPAKTAAPPVHTTALRGLRKIHTGKVRDIYEIDAHSMLIV
                     TTDRLSAFDVVLPDPIPDKGRVLNSISNFWFDRTKHIVPNHLTGRALSEVISDPDELA
                     LLADRAVIVKRLKTVPLEAVVRGYLIGSGWKDYQKSGAISGIKLPAGLKQAGRLPQTL
                     FTPSTKAAIGDKDETISFGQAADLVGKDIAARVRDTAIAIYEFAAKYALERGIIIADT
                     KFEFAQGSDGSLVLIDEVLTPDSSRFWPADTWREGTSPPSFDKQFVRDYLETLDWNKT
                     APGPKLPPEVIAGTRAKYLEALTRLTG"
     gene            11598..12425
                     /gene="djlA"
                     /locus_tag="VEW08_06410"
     CDS             11598..12425
                     /gene="djlA"
                     /locus_tag="VEW08_06410"
                     /inference="COORDINATES: protein motif:HMM:NF006948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HYJ40403.1"
                     /translation="MSITGKVVGVIVGLLIFRSPWGAIIGLILGHFYDQSVVTVRRTG
                     GGAGVLEIGERFFTATFEVMGHVAKADGRVSEAEIAAARKVMAELRLNGVQIHAAIAH
                     FTRGKSRDFDLQTTMQEFTEACANRPDLVRVFLEVQVRAALEGVDMKGPAALAIQKVA
                     DLLDVSRIELAHMEAVLRLRREQFKAGEGNGNRGGSAGQAQPRSGAQISAAYQILEVD
                     PKATDEEVAKAYRRQLSKHHPDKLKANGLPDSMLEHAKQRTQQIIEAYELIKSVRGA"
     gene            12437..13117
                     /gene="rpe"
                     /locus_tag="VEW08_06415"
     CDS             12437..13117
                     /gene="rpe"
                     /locus_tag="VEW08_06415"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005803358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HYJ40404.1"
                     /translation="MSSKHAPWIAPSILSADFARLGDEVNAVLAAGADVIHFDVMDNH
                     YVPNLTIGPLVCEGLRKHGITAPLDVHLMVKPVDRIIPDFAKAGATYISFHPEASEHV
                     DRTIELIRENGCKPGLVLNPATPLSCLDHTLKKLDLVLLMSVNPGFGGQKFIGSVLDK
                     VRSVRKLIDASGRDIRLEIDGGVKVDNIAEIARAGADMFVAGSAIFGAKDYKATISAM
                     RDQIATAK"
     gene            complement(13151..14065)
                     /locus_tag="VEW08_06420"
     CDS             complement(13151..14065)
                     /locus_tag="VEW08_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ40405.1"
                     /translation="MRKAALRFALMAALVGTPALAQQPAPQPSLVREGVTEKLTRYVW
                     AIPDGGASLVPNVGIVVGKKAVLVIDTGMGTRNAETVLREVAKVGAGKPIYLVTTHVH
                     PEHDMGAHAFPRDSKLIRSKDQIEDIAAGAGMNLVPVFAQRSALNVELLEGAKHREAD
                     IVFDRDYTLNLGGVSAKIYAMGTNHTHGDTVILVDGVLFSGDVAMRPQPSFANPTAKI
                     SHWLESLDRLEALKPKQLVPSHGSFGDTSIIGGYRGYLTHIRDRAAELKKAGKTQDEA
                     VQIITDEMSRQYPDKNRLAGAIRAAYSE"
     gene            complement(14304..14939)
                     /gene="crp"
                     /locus_tag="VEW08_06425"
                     /pseudo
     CDS             complement(14304..14939)
                     /gene="crp"
                     /locus_tag="VEW08_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016506431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
     gene            complement(15073..15741)
                     /gene="coq7"
                     /locus_tag="VEW08_06430"
     CDS             complement(15073..15741)
                     /gene="coq7"
                     /locus_tag="VEW08_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004720993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HYJ40406.1"
                     /translation="MAPQLDSLLSAADRALRALLAPPAGGRPVPPSATPGATPVTGRD
                     AQKADSALSAEDKRESAALMRVNHAGEMAAQALYHAQALFARDPEIREFMLHAAREET
                     DHLAWCETRLKELGGRPSVLNPLWYAGSFGIGALAALLGDRASLGFVAETERQVEGHL
                     KSHLERLPAEDLRSRAIVEAMCHDEVGHGQQAQSAGAATLPGPVRELMRRTARIMTHT
                     SYRL"
     gene            15912..16340
                     /gene="rplM"
                     /locus_tag="VEW08_06435"
     CDS             15912..16340
                     /gene="rplM"
                     /locus_tag="VEW08_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HYJ40407.1"
                     /translation="MTTVFAKSESVRADWYVVDAADKVLGRLATQIAMRLKGKHKAIF
                     SPHVDMGDHIVVLNAEKIKVTGNKLEDKKYYHHTGAIGGIKEIALGKLLKEHPERAIE
                     FAVKGMLPKGPLGRKMYKKLHVFAGDKHPHAAQQPKQLEI"
     gene            16353..16751
                     /gene="rpsI"
                     /locus_tag="VEW08_06440"
     CDS             16353..16751
                     /gene="rpsI"
                     /locus_tag="VEW08_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HYJ40408.1"
                     /translation="MPATAKPNYGTGRRKTAAARVYMKAGTGKITVNERPLDEFFGRE
                     TGRMIVRQPLELVQLANKFDFTVSVSGGGITGQAGAIRHGITRALMEYDPELRKALRI
                     AGFVTRDAREVERKKVGRRKARRGPQYSKR"
     gene            16785..16858
                     /locus_tag="VEW08_06445"
     tRNA            16785..16858
                     /locus_tag="VEW08_06445"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16817..16819,aa:Gln,seq:ctg)
     gene            16923..18710
                     /locus_tag="VEW08_06450"
     CDS             16923..18710
                     /locus_tag="VEW08_06450"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HYJ40409.1"
                     /translation="MKRFALLASMFLGAATSGVVHAAESRAAQLNTMYAEFWEEYLKL
                     NPITATFAGDPRYNAELPNFLTTGFEQQSKSFHKKYLERAKAIGTDGLTGQDRLSYDI
                     FTLNRESTLEQFEFPDRLLPIDQFQNIANYFAQFGSGTSAQPFATVKDYDDWLKRAVL
                     FSAVLDQAIVNMREGVKAGIVQPRVLMEKVLPQLDANIVDDVEKSIFWGPITNLPKDF
                     SAADRERLTAAFRTLFGTQVIPTYKRLRAYIADEYLPRTRATFGMGALPNGAAWYAHR
                     VHDNTTHSMTPAQVHQIGLDEVARIQDGMRAVAKDLGYTKPTKSLLELKAFFEWMKAR
                     NDMYFSSREELLSAYQKFYSDVDPLLPAYFNLRPKTSYEVRLVEPFREASASSGQYQG
                     PSLDGKRGGVFFVNAYDLKARPRWALASLSLHEAAPGHHFQISLQRELGDLPMFRRFS
                     SDTAYIEGWGLYAEYLGYEMGIYKDPVARFGALDAELWRSIRLVTDTGIHFKGWTRQQ
                     TLDYMYANSPAEETRAVSEAERFAAIPGQALAYKIGQLKIVELRKRAEKALGAKFDVK
                     KFHDEVLKDGAIPLDVLDAKIDRWIKANR"
     gene            complement(18770..20038)
                     /locus_tag="VEW08_06455"
     CDS             complement(18770..20038)
                     /locus_tag="VEW08_06455"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYJ40410.1"
                     /translation="MNVASKVRTLLAAATLSLLAANAQADTVRDGFSVERLQRMDALL
                     DGYVNDGRIGGIVALVLRDGKPVYQHATGWRDKEAGQKMTMDTIFRIASQSKALTSVA
                     ILSLMEEGKLTVNDRAGKWIPTFERTSVLTRVENSTTWTTTAAKRPITIKDLLTHTAG
                     INYGQQPEFNSLYEPKGLGLAAGIGWYFADKDEPICTTMERLGTLPFEAQPGERFVYG
                     YNTDVLGCIVEKASGVSLDEYIRSRITGPLGMPDTVFYLPADKAGRLAVVYGSGPGGK
                     IVKAPEGPRGQGDYVKGPRKSYSGGAGLVSTARDYATFLEALRNGGALGKVRILSPHA
                     VKLMTTNQIADLKSPRGLGFGYGFETHDKYGVSGMSSVGSWGWGGAYGTWYRVDPEER
                     LTIVLMLQMIPNDTDIRDKFDATLFQALID"
     gene            complement(20055..21617)
                     /locus_tag="VEW08_06460"
     CDS             complement(20055..21617)
                     /locus_tag="VEW08_06460"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HYJ40411.1"
                     /translation="MTKRILLAIVATLSCAVHGASNPEPVSAQLTIRADQPGAKIDAN
                     IYGQFMEHLGRNVYEGIWVGENSTIPNTRGFRNDTLAALKKLKVPVLRWPGGCFADEY
                     HWRDGIGARDKRPHRVNTFWGGVIEPNEFGTHEFFELAEMLGAKTYLAVNVGSGTVEE
                     MSQWVEYITSPSQSALANERRKNGRDKPWKIDYIGVGNEPWGCGGDMRAEYYTDEYKK
                     YALNIKAPRDSAPIEVASGPYGDGYDWTEVVMKNAVRQMGAYSLHYYTLPSGSFETKN
                     KGPALSFNEADWIITLKHTLAIDEMVKKHSAIMDKFDPQKKVALYVDEWGTWYDVEPG
                     TNPGFLFQQNTLRDAVVAGLNLNVFHSHADRVRMTNIAQMINVLQAMILTDKEKMLLT
                     PTYHVFEMYQPFQNATLLPTELMTPEYKHGEVAIPAVSVSAARATDGQIALALVNTDP
                     NRPARVTLKIAGGSAGKASARVLTTAAMNAHNTFDKPNNVQPAVFNGAKRKGDVWVFE
                     LPAKSVVVATLN"
     gene            complement(21678..22130)
                     /locus_tag="VEW08_06465"
     CDS             complement(21678..22130)
                     /locus_tag="VEW08_06465"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HYJ40412.1"
                     /translation="MFLRALFAFLVLPGVVALLIPATIGIRELNTGGRFAPVGLLPLA
                     AGLALLLWCVRDFYVSGKGTLAPWSPPQHLVCVGLYRYSRNPMYVAVTMMLAGWALCF
                     WSTTLAIYTCFVIVAFHLRVVFGEEPWLARTHGAAWDEYKSKVPRWLL"
     gene            complement(22138..22821)
                     /locus_tag="VEW08_06470"
     CDS             complement(22138..22821)
                     /locus_tag="VEW08_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYJ40413.1"
                     /translation="MFDLFIGNKNYSSWSLRPWVLLRELNIPFRENLVPFGGSQNPHA
                     FRDFSPTGKVPCLVDGSITVWDSLAITEYVGERHAGVWPAEAVARAWARSASAEMHSG
                     FFGLRNTCTMNCGLRIKLRTIDAALNRDLARLEELWADGLNRFGGPFLTGPRFCAVDA
                     FYAPVALRIQTYDPPIGSGARRYAAQLLALASMKDWYSAALQETWRDEEHEVEARAVG
                     EWREDLRAR"
     gene            complement(22827..23315)
                     /locus_tag="VEW08_06475"
     CDS             complement(22827..23315)
                     /locus_tag="VEW08_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ40414.1"
                     /translation="MQISTETSPGTEAVSIQVAAPSDVEVLLPMVEQYWRFEAIEGFD
                     ATRMRKLLTRVLEDQTLGRAWVATLYGEPAGYLLAVYVFSLEHQGLTAEIDEFFVVPA
                     HRGLGLGHQILAIAEAQFRLEGCTNVSLQLGRSNEAARNFYRRHGFDDRAGYELVSKN
                     LK"
     gene            complement(23354..24652)
                     /locus_tag="VEW08_06480"
     CDS             complement(23354..24652)
                     /locus_tag="VEW08_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ40415.1"
                     /translation="MAGWATAWLGGVALAALGALATARYFIASPWLATVAAALVLTPL
                     VAWSGSVLAARWSRMARALNDGITSLKDHDFSVSVTRATQDEMGDLVTTYNTLGDRLR
                     VERQSLYQRELMLDTVIQTTPLALVLTNEGDAVLYSNTAARQLFGAGRKLEGERFIRF
                     LEAAPAPLREAIERGGDTLFTIELGGEPQVYHVSQRRFQLNALPHRLLLLKQLTREIN
                     SQEVATWKKVIRVIAHELNNSLAPISSLAHSGQILARGPPDPEQLNRVFATIEERARH
                     LAGFIEGYSQFAKLPQPRVAPVAWDVLLERLRVVVQFTPVGTPPHRAISFDAAQIEQA
                     LINLLKNARESGSEAADIELAVSLAGGGCTVEIRDRGPGFSVAALENALVPFYSTKQT
                     GTGLGLTLCREIVEAHGGRLRIANRDGGGAIVALWLPDGN"
     gene            complement(24716..26140)
                     /locus_tag="VEW08_06485"
     CDS             complement(24716..26140)
                     /locus_tag="VEW08_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYJ40416.1"
                     /translation="MSAACNPKAFLGVLRMTSVTSHVEDRLAHRTVLVADDSEAVRTA
                     FQVLLSLHGARVLGASSPAEALATVRSQEVDLIIQDMNFRREATSGEEGVALFRELRA
                     LHPDVPVILLTAWTHLETAVDLVKAGAADYIAKPWDDARLLTTVRNLLELRAAVEENV
                     ATRVQRAEARARLAERYDLRGLVYESDALHTVASMAAQVAHADVPVLITGPNGAGKEV
                     LADIVHANSARKSQPYVKVNAGALPDQLIEAELFGTEAGAFTGAKQRPGRFEAADGGT
                     LFLDEIGNLPLAGQAKLLRVLQTGEYERLGSNTTRRASVRIVAATNMPLRDAMREGRF
                     REDLYYRISVIELALPPLAERRDDVLPLTRAFLPVGARLTSDAERALLNYSWPGNVRE
                     LRNVLQRAALLAGEAPINAAALNLPAVLPARSDDPVLDRATIERVLAENGSIVAQAAR
                     QLGISRQALYRRMEKLGIKESPSR"
     gene            complement(26171..27385)
                     /locus_tag="VEW08_06490"
     CDS             complement(26171..27385)
                     /locus_tag="VEW08_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYJ40417.1"
                     /translation="MGIRPIFSALLRNRAGAILVALQIAITLAIVVNAVYLTQQRVSK
                     IGRPSGIDDQNIFAFWVNTYQKDYDFLGMVREDMAMLRQMPDIVDVSPMQQVPLSGSG
                     SSSGFYSLPDKKGEESPANTYSVDDHGIKTLGVTLSAGREFDASDVEYKKEDQKEPGY
                     PPKAIISQAFAKALFKDEPALGRNFYDDQSRPIRIVGVISHMQGSWVGWDKVDRTMLY
                     PQVGPYGSTQYVIRTRPGSIDRVMANVEVALRKRDPNRVIATPMTKMSDMKRRSYSSD
                     SLMAVTLSTVTGLVLVFSSLGIFGLATFNVNSRTRQIGTRRAVGARKLDIVQYFLAEN
                     WIVTSAGVVVGCSLALAAGYWLSTQYQLPRLDLYYLVGGVLGLWVLGQVAAWQPALKA
                     AKVSPAMATRNV"
     gene            complement(27388..28716)
                     /locus_tag="VEW08_06495"
     CDS             complement(27388..28716)
                     /locus_tag="VEW08_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ40418.1"
                     /translation="MFAYYLRLALASFRRNPGLTALMVFAIALGISVCMITLTTYRAA
                     ANNPAGERSGIVFAASIDNWDPEEPYEEYDKSLAPTMLTYRDARALHASNIPDRKVIM
                     YRSGEIYTRADKGMDPQYFATRMTTSDFFPMFEVPFLYGGPWDAKADAGPEPVVVLSK
                     EANQKAFGGINSVGKTLQWAKREFRVIGVLDEWEIMPKYFDVSNGAFQKMEGAYMPFS
                     WGQILELGSHGNTNCWKTEVVDSFQAFLNSECIWTHAWFELRTAEKQRAFREFLDNYV
                     LDQKKHGRFPRPLNNYLYDVDGWLKRNKVVGDDNKAMLVLAFAFLAVCLVNTVGLLLA
                     KFLNAAPTSGVRRALGASRRDIFWQHLTESGVVALAGGLVGGLLGIAGTWALRAWYGR
                     FVDEALRALPFDSTNFLIIIAIAVAAGVLAGLYPAWRIGRAAPASYLKVQ"
     gene            complement(28720..29451)
                     /locus_tag="VEW08_06500"
     CDS             complement(28720..29451)
                     /locus_tag="VEW08_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ40419.1"
                     /translation="MLSMKNVSKVFRTDLIETHALRDFSLEVKAGEFLAVTGPSGSGK
                     TTFLNIAGLLEPYETGSYKLDDVEVTALSDDARSALRNQKIGFIFQSFNLMPDLNVYD
                     NVDVPLRYRRMKFAERDKRITNALEIVGLAARMKHHPSQLSGGQQQRVAIARAIAGDP
                     KLVLADEPTGNLDSLMARQVMDLLERINEMGTTIIMVTHDPELARRAHRNVQVVDGQI
                     SDFKPYEPHAAPVPVSVIAPAPVGA"
     gene            complement(29463..30755)
                     /locus_tag="VEW08_06505"
     CDS             complement(29463..30755)
                     /locus_tag="VEW08_06505"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HYJ40420.1"
                     /translation="MSLLLPEIAMRGTESQDRTVTASRSKRTFLRRYGVMAGIAAALL
                     FLVVWSARAWLSSEKVIPRERIRTAVAERGPFVRDVAATGMVVAAVSPTLFAEAPGTI
                     IYKVRAGDSVRAGDVLGTVDSAALTNEYEQERATLAGTEATLNRQTIEVRRQILKSSQ
                     DTDLAKVQITAAEREFKRAEDAWGIHVIPQRDFERARDDLETARLNYAHAMETGGLEK
                     DSLQLELRSLSSQRDAQALKVARLRERVDALTLRSPVAGMVATLSQQERAQVAQNAPL
                     VTVVDLTALEIEFQVSESYANEIRSGMNAGIALDGRMFQGTVAGISPDVRNSQVTGRV
                     RFTAQPKGLRQNQRASVRIVLDERNDVLKVARSPFNDSDSKFAYVVRDDAAVRTPVEF
                     GAAAIGEIEIRNGLNVGDIVVLSDMREYKDAPSVLIGN"
     gene            complement(30917..31600)
                     /locus_tag="VEW08_06510"
     CDS             complement(30917..31600)
                     /locus_tag="VEW08_06510"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HYJ40421.1"
                     /translation="MNRREFVPGCLIASALALPVAARPAPRVPLPARASIKVAFLLGA
                     DTNVIDTAGPWEVFQDVMVMRAGTHANPFEIFTVGPDAGPVAMTGGLRVIPAYGIASA
                     PKPEVIVVPAQRAQAAALEWLRASATSADMIMSVCTGAYQLARAGLLDGLKATTHHDF
                     WDDFEHEFPKVRLQRGARFVDNGRIATSAGLTSGIDLALHVVARYLGDDAAAATAKYM
                     EHASEAWRS"
     gene            complement(31677..32330)
                     /gene="rpiA"
                     /locus_tag="VEW08_06515"
     CDS             complement(31677..32330)
                     /gene="rpiA"
                     /locus_tag="VEW08_06515"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HYJ40422.1"
                     /translation="MNKNTGKQGAARAALEFVVEGKVLGVGTGSTVNFLIDELALQRM
                     RIPGAVSSSNGSTERLKAAGIPVLDLNDVGTIDTYIDGADETNHRRQLIKGGGAALTR
                     EKIIAAASGRFICIADEGKLVDTLGAFPLPVEVIPMARSYVDRELARLGGRPVWRENV
                     ITDNGNHILDVHDLRITDPVGLESAINQIVGVVTVGLFAARPADLVILGGPSGIRRF"
     gene            complement(32634..33083)
                     /locus_tag="VEW08_06520"
     CDS             complement(32634..33083)
                     /locus_tag="VEW08_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HYJ40423.1"
                     /translation="MKTEPSTFGVDDLARRPKKTAMWDGVRNFQARNMLRDDFKKGDQ
                     AFLYHSSCDVPGIAGIVEVVRTAYPDPTAFDKKHDHYDEGSDPKNPRWLVVDVQLKRR
                     LKRVITLDELRKHEQRELDGMLLLKRGNRLSITPLSAAHWKFILSME"
     gene            complement(33049..33663)
                     /locus_tag="VEW08_06525"
     CDS             complement(33049..33663)
                     /locus_tag="VEW08_06525"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HYJ40424.1"
                     /translation="MQTKSSLRFEMLQRRRAVPRAARTTAARSIASILAHTHWLRPGK
                     RIGVYASMPQEIGTGPLIQLALTRGCDVYLPRITSLRSRMMRFVRFSHGGTRHALGMH
                     EPDGVEYLSARFLDTIFVPAVALDRRGARLGHGLGFYDRTLGFRQFRQHWRGPRLVGL
                     VYSFQVVPLVPVTATDVMMDTIVTERGIDELLAHEDRTVDLRGR"
     gene            complement(33679..33865)
                     /gene="ssrS"
                     /locus_tag="VEW08_06530"
     ncRNA           complement(33679..33865)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VEW08_06530"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(33955..34272)
                     /locus_tag="VEW08_06535"
     CDS             complement(33955..34272)
                     /locus_tag="VEW08_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HYJ40425.1"
                     /translation="MSEKEPATVTVRILEKEYFVSCPQDERADLLDSAEYLNKKMREI
                     RDTGKVVGADRIAVMAALNMANELLRLRKLDHDLQGNVTGRVKQMRERVEGALQRTRQ
                     LDL"
     gene            complement(34269..34610)
                     /locus_tag="VEW08_06540"
     CDS             complement(34269..34610)
                     /locus_tag="VEW08_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HYJ40426.1"
                     /translation="MFDAASAAVSLVALAPEYIGGKAPGALYSPPMNDTSDNNKTGKS
                     VFATEIALLERRVEELLTTVDTLKEENRALRQRQESLAAERSAFAQRNDQVRARVEAM
                     IGRLKTMEHTA"
     gene            34656..35222
                     /locus_tag="VEW08_06545"
     CDS             34656..35222
                     /locus_tag="VEW08_06545"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HYJ40427.1"
                     /translation="MLPATYDEFERVLRDARALPEPAEAHGTLAGALCSSRDYGLIEW
                     LREILPEDSPEDAALQSSVLQNVYNSMVNTLVGSDADFEPLLPDDDSPLTLRADALSQ
                     WCQGFLYGLGSGTTSDPGKVSIEAGEIIRDFTEITHVGVDADEENEENEVAFAEVVEF
                     VRVGVQLLFAELAPARGEEPEPGAASIH"
     gene            35263..>35505
                     /locus_tag="VEW08_06550"
     CDS             35263..>35505
                     /locus_tag="VEW08_06550"
                     /inference="COORDINATES: protein motif:HMM:NF017046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HYJ40428.1"
                     /translation="MPSVGREEFKRRRRALLKQMGRDSIAIVPTSPVRMRNNDVEYAY
                     RPDSDFFYLTGFVEPESVAVLVPGRPQGEYILFVRDR"
BASE COUNT         6498 a        11087 c        11397 g         6523 t
ORIGIN      
        1 cgcgaaatcg aggaccacgc gcgcgccctg cgacatgccc accagcaacg gcctggggag
       61 ttcgagcctg gtgatcaacg cgtgcaggtc ctgcacgtcg gcacgcatgg acgcttcgcc
      121 ggaggaaagt ccgaagccac ggcgatcgaa gcgcacgatg cgcagctgcg cggcgagcag
      181 cgcctgcggt tcccacatgt cgaggtcgag ggtccaccca tgcaccagca ccacggcggg
      241 gcccgcgcct tcgtcgcggt aacgcagacg ggcgccgggc acggacaggt gacggtcggc
      301 ggcgatcatg ggatttcgaa taccgaaaaa aatttcgcat tcaggctacg agtgcgaacc
      361 tgcagcgtca agcggcgtgc gcctcgatcc cgcgagaggt tgctagatct gggcgctgag
      421 cgcgatgccc ggcacgcgcg cgagctccac ggagatctcc ttgctggcct tgcgcagcat
      481 cgccaggcgc tgtttgacca gtgaggcttt cgaagcgcga cgcgagtggc tggagctgtt
      541 gagcgcggct accacgcgtc ccgcctggtc gaacaccggc accgctacgg ccacgacgcc
      601 gtacgcaagc tcgtcttcca ccgccgagta accattgcgg cggcactcct cgatgagttg
      661 gcgcagtttc ttcggttcgg tgacggtctg gctggtcagc gcgacgaact tctgcgtgcg
      721 gaaatactga tcaaggtgtt cggcgctcac gcccgcgagc aacacccgcc ccatcgaggt
      781 cacatgcgcg ggaaagcggc tgccaacgtg cgcttcgagc cgcatgagcg tacgcaccga
      841 agttcgcgcc acgtacacga tgtcattgcc gtccagcacc gagagtgcgg ccgaatcacc
      901 ggtgacacgc gccagctctt cgagatgcgt cagggtcaga tgctcgatgt ccatcgactc
      961 gagataggcc gcaccgagct cgagcacctt ggggcgcaac agaaatgcgc ggccgcgacg
     1021 cgtgacgtag ccgagctcct cgagcgtgag caggcagcgg cgcgcggtcg ccgccggcat
     1081 gcgcgcggtc gcggcgattt cactgagcgt gagcgtcgag tggtcacgcg agaaagcctt
     1141 gatgacggag aggcccttgg cgaggcctcc catgacttcg cggcgcggtg ccgatgattt
     1201 cgatggcatg cggacgatca tatttcggta tgcgaagaaa aagaaacggc cggcgcgcgc
     1261 gtcttgacgc acgaggccgg tggggtacag catgctcccg cgcgtattcg ttaatcgaac
     1321 tctatttcgc tatacgaaaa gtaatgtcgc gcgaggggct caaaaattga agatcggaac
     1381 ccaggcgcag cgcacgtcgg ccatttccgc gtccaacgcg gacgcgatcg tcgttcgcgg
     1441 gcgcaacctg tgcaccgagc tcgtgggcgt cgtcactttc accgatcacg cctggctgct
     1501 ggtggccggc gacctgccga gcgccgggca acgccgcatt ctcgacgcca cgctggtggc
     1561 catcgccgag cacggcctcg tgccgagcgt ggccgcgagt cgcatgaccc tcgctgcagc
     1621 gcccgaggcg ctgcagggtg cggtggccgc cggaatcctg ggctgtggct cggtgattct
     1681 cggagccgcg gatgccgcgg ggcggctgtt caacgccatc gtcactcggg cttcgaacga
     1741 gccgcttgaa gcggcggctc gcagcgtgct cggcgagatg cgagccgcgc gccaacccgt
     1801 gcccggttat ggccaccccc tgcacaagaa gcaggatccg cgggcggcgc gcctgcttgc
     1861 cgttgccacg gaggccggca cgtccggcaa gcacatcgaa gcggcgattc ttgtcgagcg
     1921 tctcgtgccg caaatctggg gcaagccgct ggcgatgaat gtttccgccg ccatcgcggc
     1981 ggtgctgctc gatgccggct atccactgct ggccatcaaa ggcgtgccca tgctggcgcg
     2041 caccgcgagt ctcattgcgc atctgctcga agagcagcag cgcccgatcg gtttcgtgct
     2101 cgcgcacgaa ggcgccgcgt ccatcggcta cgacggcacg tgtcccgacg gcttcgtgcc
     2161 gggcgaggag tgaccatgca gccggtactt cgcggtgtga agatcgtcga gcagggcacg
     2221 ttcatcaccg gtccctgcgc cggcatgatg ctggccgacc tcggcgccga cgtgatcaag
     2281 atcgaaagcc ccggtggcga cccttaccgc gcctaccagg gcggcctgta ctcaccgcac
     2341 ttccaggcct acaaccgcaa caagagaagc gtgtgctgcg atctcaagca atccgccgat
     2401 cgtgagttgt tcgaccagtt gatcctggac gcggacgtgt acatccagaa ctttcgtccg
     2461 ggcaccgcgg aaaaactcgg cgccggcgcg gagcggctgc gcgaactcaa cccgaaactc
     2521 gtttattgct ccatcagcgg gttcggcggc agcgggccgt acgtcgagcg gcccagctac
     2581 gattcggtgg cgcaggcctt gtccggtttc ctgagcgtcg tagtcgatga agaccggccg
     2641 cggtttctcg ggccggcgct ggcggacgcg atcaccggca tttacgccgc gttcggtgtg
     2701 ctcggggcgc tgcttgaacg ggccggcagc ggccgcggca agctggtgga agtgtcgatg
     2761 ctcgaagcga tggcgcattt cgccgtcgaa ccttttgccg cgttcttcgc gctcggtgcc
     2821 gtgccgaaat ccgccgatcg cccgcggctc gcgcaggcct acattttgcg cacctccgac
     2881 aaccggctca tcgcgatcca cctgtcgtcg ctggaaaagt tctggctggg actcgtcaag
     2941 gcgctcgagg cagatgagct cacgagcgac gcgcgcttca gcgagcgcct ggcacgcatc
     3001 agcaattacg aaacgctggg tgcggagctc gaccgtcgtt ttcgcggccg cacattgaac
     3061 gattggtcgg cgcgcctgag tgcgaacgac gtgccgttcg cgccgatcaa cggcatcgat
     3121 tcggtggtcg aggatcctca ggtcggccat ctcgggctca tcgttccggt gaacggagtg
     3181 cagcagggcg gacggcaggc ggtgagaccg ccactgcaat tcgatggcga gcgtgcacac
     3241 acggtctccg ccgcgccgct gctcgaccag catggcatcg cgattcgcgc ggcgatgacc
     3301 gaaagccggc gttggccggc gatgtccgac gacgcgccgc cgcgccgcgc aacggggtag
     3361 ctggaaatgt cagagacgag gggcaaactc tttccgacca cgctggtggg cagcatgccg
     3421 caaccggagt ggctcatcga tcgccagaaa cttgctggcc ggttcccgcc gcgggtacgc
     3481 gcgctcgagc tgtggcgcat tcccgaggcc tacctggcgg aagcgcaaaa cgacgcgacg
     3541 gtactcgcaa tccgcgccca ggagaaagcc ggactcgaca tcgtcaccga cggcgagatc
     3601 cgccgtgaaa gttattcgaa ccgattcgca acggccctcg aaggcgtgga cctcgacaat
     3661 cctggaaccg cactcgatcg cagcggtcat cccaatcccg tgccgcgcgt cgtcggtccg
     3721 atccgccgca aacaccctgt gcaggtcgat gacctgaagt tcctgcgcgc gaataccacg
     3781 cggcaggtca aggtgaccgt gcccggccca ttcaccatgg cgcagcaggc gcagattgac
     3841 gcatacggcg gcagccgcga ggccgccgcc atggactatg caaaggccgt gaatgccgag
     3901 atccgcgacc tgttcgccgc gggcgccgac gtcgtgcaga tcgacgagcc ctacatgcag
     3961 gcgcggccgg acgaggcgcg tgcattcggc ctgaaggcgc tcaaccaggc gctcgatggc
     4021 gtcaccggca cgaccgccgt gcacatctgc ttcggctacg ccgcgatcat tcacgcgcgg
     4081 ccttcggcat attcattcct gccggaactc gcgggctgca gttgccgcca ggtctcggtg
     4141 gaaaccgcgc agtcgaacct cgattgcagc gcgctgcttc cgctcaagga caagaaggtc
     4201 atgttaggcg tgctggacct cgccgacatg agcgtcgaaa cccccgagct ggtcgccgag
     4261 cgtattcgcc gcgcgctcaa atacgtgccc gccgaaaaca tcattctcgc cccggattgc
     4321 ggcatgaaat atctgccgcg ggaaattgcc gttggaaaac tcaaagccat ggtcgccggc
     4381 gccgcgatca tgcgcactga actgtcgcgc gctgcgtagg agttcacatg caccttccga
     4441 acaagttgtc gccgatggga ttgattggtg tggccgtcgc tgccgctctg gcggcaacgc
     4501 cggcgcaggc acaaacgccg ccggaagaca tagacgaagt cgtcgtcacc gcgcgcaagc
     4561 gcaacgaagc gtccaccgac gtgcccgtca ccatgagtgt gttcacgcgt gaagacatca
     4621 cggccgccgg catcgaaaag ccgggcgact tcatcgcgcg cgtgccgaac atgacgctgg
     4681 tggaaaccca gaatgccggc aacgccttcg tggtggtgcg cggcatctcg caggcgcgca
     4741 acagcgagcc gtcggtggcg gtgctggtgg atggcgtgct cgagacgaat ccggccgaat
     4801 tcaaccagga gctgttcgac atcgaacaga tcgaagtact caagggtccg cagggtgcgc
     4861 tttacgggcg caacgccatc ggtggcgcga tcatcatcca gacgcgagat ccgggtgatg
     4921 agttcgaagg gcgcgtcaag ctgggtgttg gcaatggttc gtcggtgcgc gcgcaagcgg
     4981 gagtcagtgg tccgatcggc gaagggtcgt tgcggtaccg cgcgagcttg agttactacg
     5041 ataccgacgg gtacatcccg agcacgttcc ttggcgggga ggcagatccc gtccaggatt
     5101 tctcgggccg ggtccgtctc tcctgggcgc ccagcgaaac cttctcgggc gaccttcggg
     5161 tgtcggcgag caaactcgag actcgcggtt tctacttcgt gatcccgcgc gacgacgagg
     5221 ccaacccgtt cagcacattc accacgcccc ccgatgcgaa caacgtgacc agtcccatcc
     5281 aggtcaacaa tgccggcatc gacgatcgtg acctgttcac gggcgcgctg cgcctgaact
     5341 tcgagttggg tggtggcacc ctgacctcgg tgtcggccta caacaccacc gaggagacca
     5401 tcaccggcga cgcgtacgac ttccggccgc gcgacacctc gattttcaac gtcttgttag
     5461 gcaccgatct caaccagagc cagttcctcg atctgaaatc ctacagccag gaagtgcgct
     5521 tcacgtcgaa cgacgagggg cgcgtgcgct ggatcgccgg tgcctactac gtccacaccg
     5581 acaggttcat ttccacgggc aacatggtgg ataccggcaa cggcgtgttc ccggtgtatg
     5641 aggagccgcg attcggaggc aacaatccga gcgccacgtt cctcgccgat tcgcaggaca
     5701 acgacgcgtg ggcggtcttc gccgatgtga ccttcgagct cagcgacaaa ttcgagctcg
     5761 acacggcggt acgttacgac gaggacaagc gcgagaacac caccgaaaca ccgacggcat
     5821 ttctgcctga cccttcggca tccgaaggtg aagtgcgcac gcacacctgg agcgagaccc
     5881 agcccaaggt caccttgcga tacaagccca gcgaagacgt cactttcttc ggcggctgga
     5941 gccgcggctt tcgcagcggt gggttcaacc agaccggagt tggcgccgtg gccgatgaca
     6001 acggcatcgc gggcgtgaac gatctgttcg aagccgaagt ggccgatacc tgggaggttg
     6061 gcttcaaggg cgagttcctc gacgggcgcg tcagcaccgg ggtgagcctg ttccagacca
     6121 agtcgaccaa cggctatttc ttcgtgttcc tcgcagccaa ctccacgcag aacctcggca
     6181 atctcgacgc cgactacgag ggcgcggagt tcgacatcag cgtgaaagtc accgacaact
     6241 tcgagctgtt cggcagctac ggtttcaccg acagcgagat caccgacatg gaggatcctt
     6301 ccgtgatcgg caaccaggcg ccgctggtgt ccgaagacac cttcaatctc ggagcgcagt
     6361 ggcgccagcc gatcggcagt gcgctgcacc tggcgctgcg cgccgattac cggcacaccg
     6421 gagagacctg gtgggagccg tacaatgtga cctcgcgcga tccgatcgac atcgtcgata
     6481 tgcgcctggg gctcgaaggt gagaagtggg cggtcaccgc ctggtcgacc aacctcaccg
     6541 acgaggaata caacgccgag ttctcgcccg gcggattcct gttccgtgca ctgccgattc
     6601 gttacggtct cgagttcacc tattcgttct gagctggagt agatagtcat ggccacgaaa
     6661 gcatccctgc cttcgcgctt gcaccacacc gcctacgtgt cgcgtgacct ggaggcgacc
     6721 cggaaattct acgaggacat catcgggctg ccgttgctcg cgacgtggtg cgagaaggac
     6781 ctgctgttcg gtgcggagcg cacctactgc cacgtattct tcggtctcgc tgacggcggc
     6841 gcgctggcgt tcttccagtt cgccgagccc gccgaccagg agcagttcgg gccgaagatg
     6901 ccgttctcgc cgtttcatca catcgcatta cacgtggaca aggaaaccca ggccggcatc
     6961 gagaagcgcc tcaaggacgc gggcttccag gagccgcaat cgttcgtgct cgaacatggc
     7021 tactgccgct cggtgtacgt caccgacccg aacggcatga tcgtcgagtt cacgcacgat
     7081 catcccgacg tcgagaacat caatcgcatc gcgctcggcc gggcgcactc cgagctcaag
     7141 cgctggcttg ccggcgacca cacttcgaac aacacgtttc gttgatgcgg accagtaccg
     7201 atcgaatcct gaccacgcat gtcggcagcc tgcctcgttc gcaggccgtc accgacgtgc
     7261 tgttcgcgcg cgaacgcaac gatgtgcccg acaccgcagc ggccgcgaag gtgatcgcgg
     7321 acgcggtggt ggaagtcgtg aggaaacagg tcgccgccgg cgtcgacgtg gtgagcgacg
     7381 gcgagatgag caagatcagc tacgccacct acatcgccga tcgcttcacg ggcttcgacg
     7441 gtgatactcc gcgcgagccc ggccaggacc tggtggagtt tccgcggttg ctcgagaagc
     7501 tagcgaagct cggttcgacg gcgaaatacc gcaggccgcg ctgcgtcggc gagattcgca
     7561 acaagacatt cgcgccattg caggaggatc tggcgaactt cgggcgtggc gtgaaggcag
     7621 ggggcccaac ggacgcgttc ctcaatgccg cctcaccggg cgtgattgcg ctgttccagc
     7681 ccaatgattt ctacaagacc gccgacgatt acctggaagc cgtggcggaa gcgttgcgtg
     7741 gcgagtatga agccatcgtc gccgccggat tcctcgtgca gatcgacgcg cctgacctcg
     7801 gcatgggtcg gcacaccatg tatcgcaatg ccaccgtcgc cgaatatctc gcgcgcgcgg
     7861 aacggcacgt cgaagttctc aaccacgcgt tgcgcaacgt gccggcgtcg agcgtgcgca
     7921 tgcactgctg ctggggcaac tacgagggtc cacatcacca cgacgtgcca atgcgcgacc
     7981 tgctgcccgt gctgctcaag gccaggccgc aggcactgct gttcgaagcc gcgaatccgc
     8041 gtcatgcgca cgagtggacg gtattccgcg atacgcgcct gccggacgac aagatcctga
     8101 tcccgggtgt gctgagctcg acgaccaact acgtcgaaca cccggaactc gtcgccgagc
     8161 ggctgctgcg cttcgccgat atcgtggggc gcgagcgcgt catggcgggc tcggattgcg
     8221 gcttcagcac ctttgcgggc tttggtgccg tcgatccgga catcgtctac ctgaagctcg
     8281 ccgccatggc cgaaggcgcg cggctcgcga gccagaaact ttggagttag aggtgccggg
     8341 ccggattgaa gtccggcaat gcgacgcagt gggttcggtg gtggagtttt cccggtttcc
     8401 cggcccggca ccgtcagcgg caccgtcagg attcctcgat gaggtcccgc agcgtcattt
     8461 ccccgcagct cttgcgccag gcgtcgtcca tcgtcttgct gatggcgtcc accgccggaa
     8521 tcttgagatt gcgccagccg acctggccgg ccttctcgtt gcgcgcgatg tcgacgattt
     8581 cctgaatcga gatctggccg agatcgcgcg ccggcagcag cttctcgtcg tcggtcatgg
     8641 tgagcaaatg cgcatcttcg agcccgtgca cgatgcgcga gatggcgatg ccgggcatgg
     8701 caagctcgtg cgctagggtg tcgaccgacc agcgcgtctt gccatcgtga tacgccctgg
     8761 cgatgaggaa catcatcttg agcgtgagct gctcgcgttg aacggccgac atgcgcaact
     8821 ccgtgagccc catgcgcagg tagttgcggt tctgcacgta gaaggacaac tgcgcgccga
     8881 tcagcaggat cagccagccg aaataggtcc acagcagcgc ggccacgatg atggcaaagc
     8941 ccgcgtacac caccgtgagg cgcgtggaat acaccacgag ggcggtgaac acattgccca
     9001 cggccgccca gagaatgccg gcggtgaggc cgccgatgag cgccggcttc cacttcaccc
     9061 tggtgttggg cacgaacatg tacaccgcgg tgaaaatggc cgcgaccatg aagtagggcg
     9121 agatccattt gctgaactgc accacccgct cgagaaacgg catgtagcgg ccgattccca
     9181 ccgacgcact ctcgagcgcg ttgtgcgaca ggccgaggaa gctgaccacc acgatcgggc
     9241 cgacgatgag caacgcgacg tattcggtga cgcgccgcgt gaagctgcgg gcgtgctcga
     9301 cgcgccacag gaaattgaag ccgtcttcga ctttcttgat ggtgcccagc agggtccaca
     9361 gcagcaacgc gagacccacg gagccgacga gcccggtgct gacgctgtcg gcgaattcca
     9421 tgaccttcgc cgtgaattcc gcggcgctcg aacccatggg cttgaagaac tcgaggatga
     9481 cgggttgcag ctcgcgatgg gcgccgaagg ccttgagcac cgcgaacgca aaggcgatga
     9541 gcggcaccag cgacagcagc gtggcgtaca ccaggcccat ggcgtacagg ttgatctgcc
     9601 cgcgcgagag atcgcgcaac aatgcatagg ggtagcggag caaccgcagc gccagcgcgc
     9661 ccggggtctt cccttcggag aactcgccga agagacattt gtcgagccag ttccagaagg
     9721 cgactatcat tttcttgccg cagaataagt ggcccgcagc cctaagtcac gattagattg
     9781 gtcccatggg cagcacctat ccgacacgca tcgtgtgtct aaccgaagag acgactgaaa
     9841 cgctctacct gctcggcgag gaagcgcgca tcgtcggaat ctccggtttc accgtgcgac
     9901 cgccgcgcgc gcggcgtgag aaaccgcgcg tgtccgcatt cacgagcgca aagatcgaca
     9961 ggatcgtcga actgaagccc gacctggtgc tgggtttttc cgacctgcag gcggatatcg
    10021 ccgcggagct gatccggctc ggcatcgaag tgcacgtgtt caatcagcgc agcgtggcgg
    10081 agatcctgcg catgatcggc atgctgggcg gaatggtggg ttgtgaggcg aaggcacgga
    10141 cgctcatcga agaacttgaa gcgggcatcg aagccgtgcg cgcgagcgcg gcgaagtttg
    10201 cgcgccggcc gcgcgtctac ttcgaggaat gggacgagcc gcagatctcc ggcatcgcct
    10261 gggtgtcgga gctggttggc atcgccggcg gcgacgacgt gtttccggag ctggcgcggg
    10321 agtcgcttgg aaagaatcgc atcatcgccg accccctcga agtgccgcgg cgggcaccgg
    10381 aggtgatctt cggttcctgg tgcggcaaga aattccagcc cgcatccgtg gctgagcgtg
    10441 ccggctggag cgacgttccc gcggtgcgag acgggcatct ctacgaggtc aaatcctcga
    10501 tcattctgca gccaggaccc gcggcgctga ccgacggcct cgccgagctg catcggcgac
    10561 tagctagctg gaacggtgga gcggtgccgg gccgggaaac cgggaaaact ccaatcaatt
    10621 agtcttgcgc cgggagtttt cccggtttcc cggcccggca ccggtcaacc ggttaaccgc
    10681 gtcagggctt cgagatactt cgcgcgcgtg ccggcgatga cttccggagg cagcttcggt
    10741 cccggcgccg tcttgttcca gtcgagcgtc tcgagatagt cgcgcacgaa ctgcttgtcg
    10801 aacgacggcg ggctggtgcc ctcgcgccag gtgtcggcgg gccagaaacg cgaggaatcg
    10861 ggcgtgagca cttcgtcgat gagcaccagg gagccgtcgg atccctgcgc aaactcgaac
    10921 ttggtgtcgg cgatgatgat gccgcgctcg agcgcgtatt tcgccgcgaa ctcataaatg
    10981 gcgatggccg tatcgcgtac gcgcgcggca atgtccttgc ccacgaggtc cgcggcctgc
    11041 ccgaagctga tggtttcgtc cttgtcgccg atggccgcct tggtcgaggg cgtgaacagc
    11101 gtctgcggca aacgccctgc ttgtttcaag ccggcgggta gtttgatgcc ggagatcgcg
    11161 ccggacttct ggtagtcctt ccagcccgag ccaatcaggt agccgcgcac cacggcctcg
    11221 agcggcacgg tcttcaggcg tttgacgatg acggcgcggt cggcgagcag ggccagctca
    11281 tcaggatcgc tgatgacctc ggacaaggcg cgtccggtca ggtggttcgg cacgatatgt
    11341 ttcgtgcggt cgaaccagaa attcgaaatg gaattgagga cacgaccctt gtcgggaatg
    11401 ggatcgggca gcaccacatc gaaggccgac agccgatcgg tggtcacgat gagcatggaa
    11461 tgcgcgtcta tttcatagat gtcgcgcact tttccggtgt gaatcttgcg gagcccgcga
    11521 agtgcggtcg tatggacggg cggggcggcg gttttggctg gtagcatagt tggcatgaat
    11581 cttatcaagc ggccccgatg agcatcaccg gaaaagtcgt cggcgtcatc gtcggtctgt
    11641 tgatcttccg gagtccctgg ggcgccatca tcggcctcat cctcggccac ttctacgacc
    11701 agagcgtcgt gaccgtgcgc cgcacgggcg gtggcgccgg cgtactcgaa atcggcgagc
    11761 gcttcttcac ggccacattc gaagtcatgg ggcacgtggc caaggccgat gggcgcgtct
    11821 cggaggcgga gattgccgcc gcgcgcaagg tgatggcaga gctgcgcctc aacggcgtgc
    11881 agatacatgc ggccattgcg cacttcacac gcggcaagag ccgggatttc gacctgcaaa
    11941 ccaccatgca ggagttcacc gaagcctgcg cgaatcggcc ggacctggtg cgcgtattcc
    12001 tcgaggtgca ggttcgcgca gcgctggaag gcgtggatat gaaaggcccg gcggccctcg
    12061 cgatacagaa ggtggcggac ctgctcgatg tgtcgcgcat cgagctcgcg cacatggagg
    12121 ccgtattgcg tctccgacgc gagcagttca aggcgggtga gggcaacggc aatcgtggcg
    12181 gttccgccgg gcaggcccag ccgcgcagcg gcgcgcagat cagcgctgcc taccaaattc
    12241 tcgaggtgga cccgaaggca acggacgagg aggtcgccaa ggcctatcgt cgccagctct
    12301 ccaaacatca tccggacaaa ctcaaggcca acggcctgcc cgactccatg ctcgaacacg
    12361 ccaaacaacg tacgcagcag atcatcgagg cgtacgagct catcaagtcc gtgcggggcg
    12421 cgtaaactcc atggccatgt catccaagca cgcgccctgg atcgcgcctt ccatcctctc
    12481 cgcggatttc gcgcgtctcg gcgacgaagt gaacgcagtg ctcgccgccg gtgccgacgt
    12541 catccacttc gacgtgatgg acaatcacta cgtgcccaac ctcaccatcg gtccgctggt
    12601 gtgcgagggg ctgcgcaagc acggcatcac ggcgccgctc gacgtgcacc tgatggtcaa
    12661 gcccgtcgat cgcatcatcc cggacttcgc gaaggccggg gccacctaca tttcgttcca
    12721 tcccgaagcc agcgaacacg tggatcggac catcgagctg atccgtgaaa atggctgcaa
    12781 gccgggcctg gtgctgaatc cggcgacccc gctttcttgc ctcgatcaca cgctcaagaa
    12841 gctggatctc gtgctgctga tgtcggtgaa tcccggcttc ggcgggcaga agttcatcgg
    12901 cagcgtgctc gacaaggtgc gcagtgtgcg caaactcatc gacgccagcg ggcgggacat
    12961 ccgcctggaa atcgacggtg gcgtgaaggt ggacaacatc gccgagatcg cacgcgccgg
    13021 cgccgacatg ttcgtggcgg gttcggccat cttcggcgcg aaagactaca aggccacgat
    13081 cagcgcgatg cgcgaccaga tcgcgacggc gaagtaacgc ggcccggcgc gacggcgctc
    13141 cgtttcccgt ctactcggaa taggctgcgc ggatcgcgcc cgcaagccgg ttcttgtccg
    13201 ggtactgcct ggacatttca tccgtgatga tctgaaccgc ttcgtcctgc gtcttgccgg
    13261 ccttcttcag ctccgcggcg cgatcgcgga tgtgggtgag gtacccacgg tagccgccga
    13321 tgatggacgt atcgccgaaa gacccgtggc tgggcacgag ttgtttgggc ttgagcgcct
    13381 cgagtcggtc gaggctttcc agccagtggc tgatcttcgc ggtcggattc gcgaacgacg
    13441 gttgcgggcg catggccaca tcgcccgaga acaacacgcc gtcgacgagg atgacggtgt
    13501 cgccgtgcgt gtgattggtg cccatggcgt agatctttgc cgagactccg ccgagattca
    13561 gcgtgtagtc ccgatcgaac acgatgtcgg cttcgcgatg tttcgcgccc tcgagcagct
    13621 cgacgttgag cgccgagcgc tgcgcgaata ccggcaccag gttcatgccc gcgccagcgg
    13681 cgatgtcctc gatctggtct ttcgagcgaa tcagcttcga gtccctgggg aaggcgtgcg
    13741 cgcccatgtc atgctcgggg tgcacatgcg tggtgacgag gtagataggc ttgcctgcgc
    13801 ccaccttggc gacttcgcgc aacacggttt ccgcgttgcg cgtccccatg ccggtatcga
    13861 tgaccaacac ggccttcttg ccaacgacga tgccgacgtt cgggacgaga gaggcgcccc
    13921 catcaggtat ggcccatacg tagcgtgtga gcttctcggt gacgccttcg cgcaccagcg
    13981 agggctgggg tgcgggttgc tgggcgaggg ccggagtccc gaccagtgcc gccatcaatg
    14041 cgaaccgtag cgctgctttt cgcatgaatg gtctccaggc gcttgcgcgc cacaacggaa
    14101 aactatttcc aggcgctcac gcgccacaac ggaaaacgcc ggccaggggc cggcgtccgt
    14161 caagcggaag aggcagggcc tctggcgttc tattcgtcgt cgtcgtcgtc ttcatcgtcg
    14221 tcgtcctcga cgcgcgtgct gcgcactgcg ccgcccttgc cgccctgcac gggtacgacg
    14281 accgggcgcg tcttcatctt cggacgcgca ttgaatacga cgatggtctt gccggtggcg
    14341 cgaagcaggc cctgatcttc cagaaccttg agtacgcggc ccgccatttc gcgcgagcaa
    14401 ccgaccaggc gcgagagctc ctggcggctc accttgatct gcatgccctc gggatgcgtc
    14461 atggcatcgg gttcgccgca cagatccatg atggcgtgag ccacacggcc ggtgacgtcg
    14521 acgaaggcca ggtcggacag tttgcggttg gtgcgatcga gccgggtcgc cagctgcgtg
    14581 gcgagctcga acaccaggcc gggactttcc gccgcaatct ggcgaaagcg cgggtaggtc
    14641 atttccgcga gctcgcagtg gttgcgcgta cgcacccagg cgctgcgcaa cggcgattcg
    14701 tggaacagac ccatctcacc aaagaactga cctttgttga gataggcgag gaccatttcg
    14761 ttgccttcct cgtcttccac catcacttcg accgagccgc tgatgatgta gtagaggacg
    14821 tcgggcaggt cgcccgcgtg gatcacgacc gtttttgacg gaactgtgcg aatgcggcag
    14881 taactcaaaa atcggttgat tgccggaatg tcactgaggg gttttaccgc ctcttccatc
    14941 tgaatctccc ttgctgcttg catatatcgc cctgcctgcg cattaagtct tattacataa
    15001 cttaatgcca agtcaggcaa aagaacacgg accaacagtg acccgtgtca caggcgcccc
    15061 atcaaagccc aatcaaagac gatacgaagt atgcgtcatg atccgggccg ttcgccgcat
    15121 caattcgcgt accgggcccg gtaaggtcgc ggccccggca ctttgcgcct gctggccgtg
    15181 cccgacctcg tcatggcaca tggcctcgac gatagcccgg ctgcgcaggt cttcggccgg
    15241 caatcgctcg agatgactct ttaaatggcc ttccacctgg cgctcggttt cggccacgaa
    15301 ccccaggctg gctcggtctc cgagcagggc ggcaagcgcg ccaatgccga aggatcccgc
    15361 ataccacagg gggttgagca cgctagggcg gccgcccagc tccttgagcc gggtttcgca
    15421 ccaggcgaga tgatcggttt cctcgcgggc cgcgtggagc atgaattccc ggatttccgg
    15481 gtcacgcgcg aacagggcct gggcgtgata cagcgcctgc gcggccatct ccccggcgtg
    15541 gttgacccgc atcagggcgg cagattcgcg tttatcctcg gccgaaaggg cggaatccgc
    15601 cttctgggca tctcttcccg ttaccggggt ggcgcctggg gtggccgaag ggggtacggg
    15661 ccggccaccg gcggggggcg caagcagggc ccgcagggcg cgatccgccg cggataacaa
    15721 ggagtccagt tggggcgcca tggggcggaa gtatagggag ttttccaaag cctgttttgc
    15781 aaaggctagg ggcgttgagt acactctgcg ccctttttcc aggggcccaa acagatcaga
    15841 tcgttagctg aatcttcagc tgctttctgg gtgcgaggtg tgggctcttt ccggtggttg
    15901 gagttttagt gatgactact gtttttgcga aatccgagtc tgtgcgcgct gactggtacg
    15961 tggtggatgc cgcggacaag gtcctcggcc gcctcgcgac ccagatcgcc atgcgcctca
    16021 agggcaagca caaggccatc ttcagcccgc acgtggacat gggcgatcac atcgtcgtgt
    16081 tgaatgcgga gaagatcaaa gtcacgggca acaagctcga agacaagaag tactaccacc
    16141 acactggcgc catcggcggc atcaaggaaa tcgctctcgg caagctgctc aaggagcatc
    16201 ccgagcgcgc catcgaattc gccgtgaagg gcatgctccc caagggcccg ctcggtcgca
    16261 agatgtacaa gaagctgcat gtgttcgcgg gcgacaagca cccgcacgcc gcccagcagc
    16321 cgaagcagct cgaaatttaa ggaactgaaa gaatgcccgc tactgcaaag ccgaactacg
    16381 gcaccggccg ccgcaagacc gccgccgccc gcgtctacat gaaggccggc accggcaaga
    16441 tcacggtgaa cgagcgtccg ctcgacgaat tcttcggtcg tgaaaccggt cgcatgatcg
    16501 ttcgccagcc gctcgaactc gtgcagctcg ccaacaagtt cgatttcacc gtgtcggttt
    16561 ccggcggcgg catcaccggc caggccggcg ccattcgcca cggcatcacg cgcgcactga
    16621 tggaatacga cccggagctg cgcaaggcac tgcgcatcgc gggcttcgtc acgcgcgacg
    16681 cgcgcgaagt cgaacgcaag aaggtcggcc gccgcaaggc gcgtcgtgga ccgcagtact
    16741 ccaagcgtta acaacgctgg tattgttcgc agacggccgg tttttgggag atcgtctagc
    16801 ggtaggacaa cggactctga ctccgtttac ctaggttcga atcctagtct cccaaccaaa
    16861 caggaaaccc gcccgcttct tcgaaagaag gggcgggttt cttttttagg gagtagttag
    16921 tgatgaagcg atttgcgttg ctcgcctcga tgttcctcgg cgccgccaca tccggcgtcg
    16981 tgcacgccgc ggagtcacgc gccgcgcagc tgaacaccat gtacgccgag ttctgggagg
    17041 agtacctcaa gctcaacccg atcacggcca ccttcgccgg cgatccccgc tacaacgcgg
    17101 aactgccgaa tttcctgact accgggttcg agcagcagtc gaagagcttc cacaagaagt
    17161 acctcgaacg cgccaaggcc atcggcaccg atggactcac cggccaggac cgcctctcgt
    17221 acgacatctt cacgctgaat cgcgagtcga cactcgagca gttcgagttt cccgaccggc
    17281 tgctgcccat cgatcagttt cagaacatcg ccaactactt cgcccagttc ggttccggca
    17341 cgagcgcgca acccttcgcc accgtcaagg actacgacga ctggctgaaa cgcgcggtgc
    17401 tgttttccgc agtgctcgat caggccatcg tgaacatgcg cgagggcgtg aaagccggca
    17461 tcgtgcagcc gcgtgtgctc atggagaagg tgctgccgca gctcgatgcc aacatcgtcg
    17521 acgacgtgga gaagagcatc ttctggggtc ccataacgaa ccttccaaag gatttttccg
    17581 cagcggaccg tgaacgcctg accgcggcat tccggacttt gttcggcacg caggtcattc
    17641 cgacctacaa gcgcctgcgc gcctatatcg ccgacgagta cctgcccagg acgcgcgcga
    17701 ccttcggtat gggcgcactg ccgaacgggg ctgcctggta cgcccaccgg gtgcacgaca
    17761 acaccacgca ttcgatgaca cccgcgcagg tgcatcagat cggccttgat gaggtggcgc
    17821 gcatccagga cggcatgcgc gccgtggcga aggacctcgg ctataccaag ccaacgaagt
    17881 cattgctcga actcaaggct ttcttcgaat ggatgaaggc gcgcaatgac atgtacttct
    17941 cctcgcgcga ggagttgtta tccgcatacc agaagttcta cagcgacgtg gatccgctgc
    18001 tgccggccta tttcaatctg cggcccaaga ccagctacga agtgcgcctg gtcgagccat
    18061 tccgcgaagc ctcggcgtcc tccggccagt accagggccc ctcgctggat ggcaaacgcg
    18121 gcggcgtttt cttcgtgaac gcctatgacc tcaaggcccg tccgcgctgg gcgctggctt
    18181 cgctgtcgtt gcacgaagcc gcgccgggcc atcatttcca gatctcgttg caacgtgagc
    18241 tcggcgacct gccgatgttc cgccgcttct cgagcgatac cgcctacatc gaagggtggg
    18301 ggctatacgc cgagtacctt ggctatgaaa tgggcatcta caaggatccg gtggcgcgct
    18361 tcggcgcgct cgatgccgag ttgtggcgct cgatccgcct cgtgaccgat acggggattc
    18421 atttcaaggg atggacgcgc cagcagactc tcgactacat gtacgcgaac tcgccggcgg
    18481 aggagacgcg cgccgtgtcc gaagcagaac ggtttgccgc cattcccggc caggcgctcg
    18541 catacaagat tggccagttg aagatcgttg aactacgcaa gcgtgccgaa aaggcgctgg
    18601 gtgcgaagtt cgacgtaaag aagttccacg acgaagtgct gaaagacggc gccattccgc
    18661 tcgacgtgct ggacgccaag atcgatcgct ggatcaaggc gaatcgctga tcggagtgaa
    18721 aaaggggtca ggcaccgttt tccaggaaac ggtgcctggc accatttttt cagtcgatga
    18781 gggcttgaaa aagcgtcgcg tcgaacttgt cgcggatgtc cgtgtcgttc ggaatcatct
    18841 gcagcatgag cacgatggtc aggcgctctt cgggatccac gcgataccag gtgccgtacg
    18901 cgccacccca gccccaggaa cccaccgacg acatgccgct cacaccgtac ttgtcgtggg
    18961 tttcgaagcc atagccgaat cccaggcccc gcgggctctt gagatcggcg atctggttgg
    19021 tggtcatgag cttcactgca tgcggtgaga ggatgcgcac tttgcccagt gcgccgccat
    19081 tacgcagcgc ctcgaggaag gtggcgtagt cgcgcgccgt ggaaaccagg ccggccccgc
    19141 ccgagtagct cttgcgtgga cccttgacgt agtcaccctg gccgcgaggc ccttcgggtg
    19201 ccttcacgat cttgccgccg ggaccgctgc cgtacacgac cgcgagccgg ccggccttat
    19261 cggcgggtag atagaacacg gtgtccggca tccccagtgg cccggtgatg cgtgagcgga
    19321 tgtactcatc cagggaaacg cccgaagcct tctcgacgat gcacccgagc acatcggtgt
    19381 tgtagccata cacgaagcgt tcgccgggtt gcgcctcaaa gggcagggtc ccgaggcgct
    19441 ccatggtcgt gcagatgggc tcgtccttgt cggcgaaata ccagccgatg ccagccgcca
    19501 gccccaggcc cttgggttcg taaagcgaat tgaactccgg ctgctggccg tagttgattc
    19561 ccgcggtgtg ggtgagcagg tccttgatgg tgatggggcg cttcgcggcg gtggtggtcc
    19621 aggtggtgct gttctcgacg cgcgtgagca cgctggtcct ctcgaaagtg ggaatccact
    19681 ttccggcgcg atcgttcacc gtgagtttgc cttcctccat gagcgaaagg atggccacgc
    19741 tggtcagcgc cttggactgc gaggcgatgc ggaagatggt gtccatggtc atcttctggc
    19801 ccgcttcctt gtcgcgccag cccgtggcgt gctggtacac gggttttccg tcacgcagta
    19861 ccagtgcaac gatgccgccg atgcggccgt cgttcacata gccatcgagc aacgcgtcca
    19921 tgcgctgcag gcgctcgacg gagaagccat cgcgaaccgt gtccgcctgc gcgttggcgg
    19981 ccagcagcga cagtgtggcg gcggccagca gagtgcgcac tttcgaagca acgttcatcg
    20041 tgaaacctcg ttcatcagtt cagggtggcc acgaccacgg attttgcagg caactcgaac
    20101 acccatacat cgcccttgcg cttcgcgccg ttgaatacgg cgggctgcac gttattgggc
    20161 ttgtcgaacg tgttgtgtgc gttcatggcg gctgtggtga gcacgcgggc ggatgctttg
    20221 ccagcgctcc cgcctgcgat cttgagcgtc acgcgcgcgg gccggttggg gtcggtgttc
    20281 accagcgcca gcgcgatctg gccgtccgtg gcgcgcgcgg ccgagacact gaccgccgga
    20341 atcgccacct cgccgtgctt gtattccgga gtcatgagct ccgtgggcag cagcgtggcg
    20401 ttctgaaagg gctggtacat ctcgaacacg tggtacgtgg gcgtgagcag catcttttcc
    20461 ttgtcggtga ggatcatcgc ctgcagcacg ttgatcatct gcgcaatgtt ggtcatgcgc
    20521 acgcggtccg cgtggctgtg aaacacgttg aggttgagtc ccgcgacgac ggcgtcgcgc
    20581 agcgtgttct gctggaacag gaagccgggg ttcgtgccgg gctcgacgtc gtaccacgtg
    20641 ccccactcat ccacgtaaag cgccaccttc ttctgcggat cgaacttgtc catgatcgcg
    20701 gagtgcttct tcaccatctc gtcgatcgcg agcgtgtgct tgagcgtgat gatccagtcg
    20761 gcttcgttga acgacagcgc cgggcccttg ttcttcgtct cgaaactgcc ggaaggcagc
    20821 gtgtagtaat gcagtgaata cgcgcccatc tgccggacgg cattcttcat gacgacttcg
    20881 gtccagtcgt acccgtcgcc gtagggtccg ctggccacct cgatgggcgc gctgtcgcgc
    20941 ggcgccttga tgttgagcgc gtatttcttg tattcgtcgg tgtaatactc ggcacgcatg
    21001 tcgccgccgc agccccaggg ctcgttaccc acgccgatgt agtcgatctt ccacggcttg
    21061 tcgcggccgt tcttgcgacg ctcattggcc agcgcggact gcgagggcga agtgatgtac
    21121 tcgacccact gcgacatttc ctccaccgtg cccgagccca cgttcacggc caggtaggtc
    21181 ttggcgccca gcatttctgc cagctcgaag aactcgtgag tgccgaactc attcggctcg
    21241 atgacgccgc cccagaacgt gttcacgcga tgcgggcgtt tgtcgcgcgc gccgatgccg
    21301 tcgcgccagt ggtattcgtc ggcgaagcag ccgcccggcc agcgcagcac cggaaccttg
    21361 agtttcttaa gcgccgcgag agtgtcattg cgaaatccgc gcgtgttcgg aatcgtcgag
    21421 ttctcgccca cccagatgcc ttcgtagaca ttgcggccga ggtgttccat gaactggccg
    21481 tagatgttgg cgtcgatctt ggcgcccggc tgatccgcgc ggatggtcaa ctgcgcgctg
    21541 acaggctcgg ggttcgacgc cccgtgaaca gcacagctca gcgtggccac gatggccaac
    21601 aggattctct tcgtcattcg attcccctct gccggtgagg ttgcggcaac gcgtcgaaga
    21661 ctagcggatt tatccgctca cagcagccag cgcggcactt tcgatttgta ttcgtcccat
    21721 gccgcgccgt gtgtgcgcgc aagccacggc tcttcgccaa acaccacgcg caaatggaat
    21781 gccacgatca caaaacaggt atagatcgcg agtgtcgttg accagaaaca caaggcccag
    21841 cccgcgagca tcatcgtgac tgcgacatac atgggattac ggctgtagcg gtacaggccg
    21901 acgcagacca ggtgctgcgg tggcgaccac ggtgccagcg tgcctttgcc ggaaacgtag
    21961 aaatcgcgaa cgcaccacaa cagcagtgcg aggccggcgg cgagcggcag caacccgact
    22021 ggcgcgaatc gtccaccggt attcagttca cgaatgccga tggtggcggg gatcagcagc
    22081 gccaccacgc cgggcagcac gagaaacgcg aagagggcgc gcaggaacat gaaccaacta
    22141 ccgcgcgcgc aggtcttccc gccactcgcc cacggcgcgc gcctcgactt cgtgctcctc
    22201 gtcgcgccag gtttcctgca gcgccgccga gtaccaatcc ttcatcgagg ccagcgccag
    22261 gagctgagcc gcatagcggc gcgcgccact gcctatcggc gggtcataag tctggatgcg
    22321 cagcgccacg ggcgcgtaga aggcatcgac ggcgcagaag cgcggtcccg tcaggaacgg
    22381 accgccgaag cgattcaggc catcggccca gagttcttcg agtctggcga ggtcgcgatt
    22441 gagtgccgcg tcgatcgtac ggagcttgat gcgcagcccg cagttcatcg tgcaggtatt
    22501 gcgaagcccg aagaagccgg aatgcatctc ggcgctggcc gagcgcgccc aggcgcgggc
    22561 gaccgcttcg gccggccaga cgcctgcgtg tcgctcgccc acgtattccg tgatggcgag
    22621 cgagtcccaa actgtgatcg agccgtcgac gaggcacggc actttgcccg tcggagagaa
    22681 gtcgcgaaac gcgtgcgggt tctgcgagcc cccgaagggc accaggtttt cgcgaaacgg
    22741 gatgttcaac tcgcgcagga gcacccaggg ccgcagcgac cacgacgagt agttcttgtt
    22801 gccgatgaaa aggtcgaaca cgggttttac ttcagattct tgctcacgag ttcatacccg
    22861 gcgcggtcgt cgaacccgtg gcgccggtag aagtttcgcg ccgcctcgtt gctgcgaccg
    22921 agctgcagcg aaacattggt gcagccctcg aggcgaaact gcgcttcggc gatggccagg
    22981 atctggtgcc ccagccccag gccgcgatgc gccggcacca cgaagaactc gtcgatctcg
    23041 gccgtcaggc cctggtgctc gaggctgaac acgtagacgg ccagtagata gccggcaggt
    23101 tcgccgtaca gcgttgccac ccacgcgcgc cccagggtct ggtcttccag cacacgcgtg
    23161 agcagcttgc gcatgcgcgt ggcgtcgaat ccctcgatgg cttcgaagcg ccagtattgt
    23221 tcgaccatgg gcagcagcac ttcgacgtcg gaaggtgccg cgacctgaat gctgaccgct
    23281 tcagtcccgg gagaggtttc ggtggagatt tgcattgcgc gattctcgca cacaggattt
    23341 cgcaggcgtc acgttagttt ccgtccggca gccacaacgc cacgatggcg ccaccaccgt
    23401 cgcggttggc gatgcgcagg cggccgccgt gcgcctcgac aatttcacgg cacaacgtga
    23461 gtccgaggcc tgtgccggtc tgtttggtcg aatagaacgg caccagcgca ttctccagcg
    23521 cggccaccga aaatccgggg ccgcgatcgc gaatctcgac ggtacaacca ccgccggcaa
    23581 gcgagaccgc cagttcgatg tcggccgcct cggaacccga ctcgcgggcg tttttcagca
    23641 ggttgatgag cgcctgctcg atctgcgcgg cgtcgaagct gatggcgcga tgcgggggag
    23701 tacccacggg cgtgaactgc actacgacgc gcaatcgctc cagcaggacg tcccatgcca
    23761 cgggcgccac gcgcggctgc ggcagcttgg cgaactgcga ataaccttcg atgaacccgg
    23821 cgaggtgccg cgcgcgttct tcgatcgtgg cgaacacgcg gttcaactgc tcgggatcgg
    23881 gcggcccgcg tgccaggatc tgtcccgaat gcgccagcga ggatatgggc gccagggagt
    23941 tgttcagttc gtgcgcgatg acgcggatga cttttttcca ggtcgccacc tcctgcgaat
    24001 tgatttcccg cgtgagttgc ttgagcagca gcagcctgtg cggcagggcg ttcaactgga
    24061 agcgccgctg cgacacgtga tacacctgcg gctcgccgcc gagttctatc gtgaacaaag
    24121 tatcaccgcc gcgttcgatg gcctcgcgca gcggcgcggg tgcggcctcg agaaaacgta
    24181 tgaaacgttc accttcgagc ttgcggcctg cgccgaacag ctggcgtgcc gccgtgttgc
    24241 tgtagaggac cgcgtcgccc tcgttggtga gcaccagcgc gagcggcgtg gtctggatca
    24301 ccgtgtccag catcagttcg cgctggtaga ggctctggcg ctccacgcga aggcgatcgc
    24361 cgagggtgtt gtaggtggtc accaggtcgc ccatctcgtc ttgcgtggcg cgggtgacgc
    24421 tcacgctgaa atcgtggtcc ttgaggctgg taatgccgtc gttgagcgcg cgggccatgc
    24481 ggctccagcg ggcggccagc actgaaccgg accaggctac gagcggggtg agcacgagtg
    24541 ctgcggcgac ggtcgccaac cacggagagg caatgaagta tcgcgccgtg gcgagcgcgc
    24601 ccaacgccgc gagcgcgacg ccgccgagcc aggcggtggc ccagccggcc atggatcctc
    24661 tgaatgccca gtgcatgaat cccgggaatc gccttttgtt agtccctggt gtcgatcacc
    24721 gggacgggga ctccttgata ccgagttttt ccatgcggcg atagagggcc tgccggctga
    24781 tgccgagctg gcgcgcagcc tgcgcaacga tactgccgtt ttcggcgagc acacgttcga
    24841 tggtggcccg atcgagcacc ggatcatcgg agcgcgcggg caagacggcc gggaggttga
    24901 gcgctgcggc gttgatgggc gcttcacccg cgagcagcgc cgcgcgttgc agcacattgc
    24961 gaagctcgcg gacgttaccc ggccaggaat agttgagcag cgcgcgctcg gcgtcactgg
    25021 tgagccgcgc gccgacgggc aggaatgcgc gggtgagcgg cagcacgtcg tcgcggcgct
    25081 cggccagcgg cggcagcgcg agttcgatca cgctgatccg gtagtacaga tcctcgcgga
    25141 agcggccttc gcgcatcgcg tcgcgcagcg gcatattggt cgccgcgacg atgcgcacgc
    25201 tggcgcgtcg cgtggtgttg gagcccaggc gctcgtattc gccggtctgc agcactcgca
    25261 gtagtttggc ctggccggcg agcggcaggt tgccgatctc atcgaggaac aacgtgccgc
    25321 cgtcggcagc ctcgaaccgg ccggggcgct gtttggcgcc cgtgaacgcg cccgcttccg
    25381 tgccgaagag ctcggcttcg atgagctggt cgggcagggc accggcattc accttcacat
    25441 agggctggct cttgcgcgcg gagttcgcat gcacgatgtc ggcgagcact tccttgccgg
    25501 cgccattggg tccggtgatg agcaccggta cgtcggcatg tgcgacttgc gcggccatgg
    25561 acgccaccgt gtgcagcgcg tcgctttcgt agaccaggcc ccgcaggtcg tagcgctcgg
    25621 cgaggcgggc gcgtgcttcg gcgcgctgta cgcgcgtcgc cacgttctcc tccacggccg
    25681 cgcgcaactc gagcagattg cgcaccgtcg tcagcaggcg tgcgtcatcc cagggcttgg
    25741 cgatgtagtc ggcggcgccg gccttgacga ggtccacggc cgtctccaga tgggtccacg
    25801 cggtcagcag gatcaccggt acatcaggat gcagcgcgcg cagctcacga aaaagcgcga
    25861 caccttcctc gccggaagtc gcttcgcggc ggaagttcat gtcctggatg atcaggtcca
    25921 cttcctggga acgcacggtc gcgagggcct cggccggaga tgaagcgccg agaacccgcg
    25981 cgccgtgcag ggacaacagg acctggaagg ccgttctcac ggcttcacta tcgtcagcaa
    26041 ccagtactgt ccggtgtgcc aggcggtcct ctacgtgtga agtgactgaa gtcatacgta
    26101 agacgcccag gaacgcttta gggttgcaag ccgcggacat atttgcactg tcgaaggatc
    26161 ggactcgagg tcacacgttt cgagtggcca tggcaggcga caccttcgcc gccttgagcg
    26221 cgggctgcca tgccgcgacc tggcccaaca cccacaggcc cagaacgccg cccaccaggt
    26281 agtacagatc gagccgtggc aactgatatt gagttgacag ccagtagccc gcggccagcg
    26341 cgagggaaca tccgaccacc acgcccgcgc tggtgactat ccaattctca gccaggaaat
    26401 actggacgat gtcgagcttg cgcgcgccta ccgctcggcg agtgccgatc tggcgcgtgc
    26461 gactgttcac gttgaatgtg gcgagtccaa aaatgcccag cgagctgaat accagcacaa
    26521 gaccggtcac cgtcgacaaa gtcacggcca tgagcgagtc gctggagtag ctgcggcgct
    26581 tcatgtccga catctttgtc atcggcgttg cgatgacgcg attgggatca cgcttgcgca
    26641 gcgcaacctc cacgtttgcc atgacgcggt cgatcgaacc aggcctggtg cgaattacgt
    26701 attgagtcga tccgtaggga cccacctgcg ggtagagcat ggtgcgatcg actttgtccc
    26761 agcctaccca cgacccctgc atgtgcgata tgacgccgac gatgcggatg ggacgactct
    26821 ggtcgtcgta gaagttcctg cccagggcgg gctcatcctt gaacagcgct ttggcgaatg
    26881 cctggctgat gatggccttc ggcggataac cgggttcctt ctggtcctcc ttcttgtact
    26941 cgacgtcgct cgcatcgaat tccctgcctg ccgacagcgt gacgcccagc gtcttgatgc
    27001 catgatcgtc cacggagtac gtgttggccg gcgattcctc ccccttcttg tccggcagcg
    27061 aatagaaacc gctggagctg ccgctgcccg ataacggcac ctgctgcatg ggtgagacgt
    27121 cgacgatgtc cggcatctgg cgcagcatcg ccatatcctc acgaaccatg ccgaggaagt
    27181 cgtagtcctt ctggtaggtg ttcacccaga acgcaaagat gttctgatcg tcgattcccg
    27241 atgggcgccc gatcttgctc acgcgttgtt gggtcaggta gacggcgttg acgactatgg
    27301 ccagggtgat cgcgatctgc agagcgacca ggatggcgcc cgcgcgattg cgcagcagcg
    27361 cggaaaaaat aggtcgaatt cccatgacta ttgcaccttg agatacgagg cgggagctgc
    27421 acgtccaatg cgccatgcgg gataaagtcc ggcaagcact ccagcggcaa cggcgatggc
    27481 gatgatgatc aggaaattgg tggagtcgaa tggcagcgca cgcagtgctt cgtcgacgaa
    27541 gcgtccgtac caggcacgca gcgcccaggt gccggcaatg cccagcagcc cgccaaccag
    27601 ccccccggcg agcgccacca cgccggattc cgtgaggtgt tgccagaaga tgtcgcgcct
    27661 gctggcgcca agcgcacggc gtaccccgga tgtcggcgcg gcattgagaa atttcgcgag
    27721 cagcaggcct acggtattga ccaggcacac cgccagaaat gcgaacgcca gcaccagcat
    27781 ggctttgttg tcgtcgccga cgaccttgtt gcgcttgagc cagccgtcca catcgtaaag
    27841 gtagttgttg agcggacgcg gaaagcggcc gtgcttcttc tggtcgagca cgtagttgtc
    27901 gaggaattcg cggaaggcgc gctgtttttc cgcggtgcgc agctcgaacc aggcatgcgt
    27961 ccagatgcat tccgaattga ggaatgcctg gaagctgtcg acgacctcgg tcttccagca
    28021 attcgtgttg ccgtggctgc ccagctcgag gatctggccc caggaaaacg gcatgtatgc
    28081 gccctccatc ttctggaatg cgccattact cacgtcgaag tacttcggca tgatctccca
    28141 ctcgtcgagt acgccaatca cgcgaaactc gcgcttcgcc cactgcagcg tcttgccaac
    28201 gctgttgatg ccgccgaacg ccttctggtt cgcctccttg ctgagcacga cgaccggctc
    28261 cggtcccgca tccgccttgg catcccaggg gccgccgtac aggaacggca cttcgaacat
    28321 cgggaagaag tccgaagtcg tcatgcgcgt cgcgaaatat tgagggtcca tgcccttgtc
    28381 ggcgcgggta taaatctcgc ccgacctgta catgatcacc ttgcgatcgg ggatgttgga
    28441 ggcgtgcaag gcacgcgcgt cgcggtaggt gagcatcgtc ggcgcgagcg acttgtcgta
    28501 ttcctcgtac ggttcctcgg gatcccagtt gtcgatggac gccgcaaaga cgattccact
    28561 gcgttcgccg gccggattgt ttgctgccgc gcgataggta gtcagcgtga tcatgcacac
    28621 cgagataccc agtgcgatcg cgaacaccat gagggcggtg agcccgggat tgcgccggaa
    28681 actggcgagt gccagtcgca ggtagtaggc gaacatggct caggctccca caggcgctgg
    28741 cgcaatcacg gaaaccggga ccggtgcggc atgcggctcg tagggcttga agtcggagat
    28801 ctggccgtcg acaacctgca cgttgcgatg cgcgcggcgc gcaagctccg gatcgtgcgt
    28861 gaccatgatg atggtggtgc ccatctcgtt gatgcgttcg agcaggtcca tgacctgccg
    28921 cgccatcagg gaatcgagat tgcccgtggg ttcatcggcg agcaccagct tcggatcgcc
    28981 ggcaatggca cgtgcaatcg ccacgcgctg ctgctggccg cccgacaact gcgatggatg
    29041 gtgtttcatg cgcgcggcca ggccgacgat ctccagcgca ttggtgatgc gcttgtcgcg
    29101 ttccgcgaac ttcatgcgcc ggtagcgcag cggcacgtcg acgttgtcgt atacgttgag
    29161 gtctggcatc aggttgaagc tctggaagat gaagccgatc ttctgattgc gcagggcgga
    29221 acgcgcatcg tcggagagcg cggtcacttc cacgtcgtcg agcttgtagc tgccggtctc
    29281 gtacggctcg agcagtcccg cgatgttcag aaacgtggtc ttgcccgaac ccgatggccc
    29341 ggtgaccgcc aggaactcac cggccttcac ctccagcgag aaatcgcgca acgcatgcgt
    29401 ctcgatgaga tcggtgcgaa acactttgct gacgtttttc atggagagca tggagattcc
    29461 tcttagttac cgatgaggac gctgggcgcg tccttgtatt cgcgcatgtc ggagagcacg
    29521 acgatgtcgc cgacattgag tccattgcgg atttcgatct cgccgatggc cgccgcgccg
    29581 aattccacgg gcgtgcgtac ggccgcgtcg tcgcgcacca cgtaagcgaa cttcgaatca
    29641 gagtcgttga aggggctgcg tgccaccttg agcacatcgt tgcgctcgtc gagcacgatg
    29701 cgcaccgagg cacgctggtt ctggcgcagg cccttgggct gcgcggtaaa gcgcacgcgg
    29761 ccggtcacct gcgaattgcg cacgtcgggg gagatgccgg cgacggtgcc ctggaacatg
    29821 cgcccgtcga gtgcgatccc ggcgttcatg cccgaacgga tctcgttggc gtagctctcc
    29881 gagacctgga attcgatttc gagcgccgtg aggtcgacca ccgtgaccag tggcgcgttt
    29941 tgcgcgacct gggcgcgttc ctgctggctc agggtggcga ccatgcccgc gaccggcgat
    30001 ctgagcgtca gcgcatccac gcgttcgcgc aggcgggcca ccttgagggc ctgggcgtca
    30061 cgctgcgagc tcaggctgcg cagctcgagt tgcaggctgt ccttctcgag gccgccggtc
    30121 tccatggcgt gcgcgtagtt gagcctggcg gtttcgagat cgtcgcgggc acgctcgaag
    30181 tcgcgttggg gaatgacatg gatgccccag gcatcctccg cgcgtttgaa ctcgcgctcg
    30241 gcggccgtga tctggacctt ggcaaggtcg gtgtcctggc tgcttttgag gatctggcgc
    30301 cgcacttcga tggtctggcg gttgagcgtg gcctcggtgc ccgcgagcgt cgcgcgttcc
    30361 tgctcgtatt cgttggtgag cgcggcgctg tcgaccgtgc ccaacacatc gccggccctg
    30421 accgagtcgc ccgcgcgcac cttatagatg atggtgccgg gtgcttcggc gaacagcgtg
    30481 gggctgaccg cggcgaccac catgccggtg gcggccacgt cgcgaacgaa agggccgcgc
    30541 tcggcgacgg ccgtgcggat ccgttcgcgc ggaatcactt tctcggacga cagccaggcg
    30601 cgcgcgctcc acacgaccag gaacagcagc gccgccgcga tgccggccat gaccccgtag
    30661 cggcgcagaa acgttcgctt gctcctggag gcggtgacgg tccggtcttg cgactcggta
    30721 ccgcgcatgg ctatttcggg cagaagcagt gacataccgg ggatagagca catcccgtgc
    30781 caagcggtcc cgaggagcgc gtcgcctgcc taactggttg aatttcccgg gcgacgcgct
    30841 cggagggttc cgcgttatcc ccacgaccgg ggtgtccgcg agcggacagc ggacaggagc
    30901 ggacgtacac atttccttac gagcgccagg cctcgcttgc gtgctccatg tacttcgccg
    30961 ttgccgccgc ggcgtcgtct ccgagatacc gagccaccac gtgtaatgcg aggtcgatgc
    31021 cggaggtgag tcctgcggaa gttgcgatgc gcccgttgtc cacgaatcgg gcgccgcgtt
    31081 gcaggcgcac cttgggaaac tcgtgctcga agtcatccca gaagtcgtgg tgggtggttg
    31141 ccttcaggcc gtcgagcaat ccggctcgag cgagctgata ggcgcccgtg catacggaca
    31201 tgatcatgtc cgcgctggtc gcggatgcgc gcagccattc gagagccgca gcctgggcgc
    31261 gctgcgccgg aacgacgatc acttcgggct tcggtgcgct cgcaatgccg taggcaggga
    31321 tcacccggag cccaccagtc atcgcaaccg gccccgcatc tggacccacg gtgaatattt
    31381 caaacgggtt cgcatgtgtc cccgcgcgca tcaccatgac gtcctggaag acttcccagg
    31441 gtccggcggt gtcgatgaca ttggtgtcgg cacccagcag gaaggccacc ttgatgcttg
    31501 cgcgcgctgg aagcggcacg cgtggggcgg gtctggcggc aacgggcaat gccaatgcgc
    31561 tggcgatgag gcaacccgga acgaattcgc gacggttcat gtgctttcct ccacggatat
    31621 tcatccgagg tgcacgccgg ggcattaccg tcgcggcgga tgcgatgagc cgagaactag
    31681 aagcgtcgaa tcccggatgg cccgccgagg atgacgaggt ccgcgggacg cgccgcgaac
    31741 aaacccacgg tcaccacacc cacgatctgg ttgatcgcgc tttcgaggcc cacggggtcg
    31801 gtgatacgca ggtcatgcac gtcgagaatg tgattgccgt tgtcggtgat cacgttctca
    31861 cgccacacag ggcgcccgcc caggcgcgca agttcgcgat ccacgtaact gcgtgccatc
    31921 gggatgactt ccaccggcag ggggaatgcg ccgagtgtgt ccaccagctt gccctcatcg
    31981 gcaatacaga tgaaccggcc ggaggctgcg gcgatgatct tctcgcgggt gagggcggcc
    32041 ccgccgccct tgatcaactg ccgccgatgg ttggtttcat ccgcgccatc gatgtaggtg
    32101 tcgatggtcc cgacgtcgtt caggtcgagt accgggatgc cggcggcctt gagtctttcg
    32161 gtggatccat tggagctcga caccgctccg ggtatgcgca tgcgttgcag cgcgagttcg
    32221 tcgatgagga agttcaccgt tgatcccgtg ccgacgccga gcacttttcc ttccaccacg
    32281 aattccagcg cagcgcgcgc ggcgccttgt tttccagtgt ttttgttcac ttcctgggca
    32341 ctcacttcgc aactccgaaa atctgaaccc tgaaacgaca ctgcccgctt ggtagcgggc
    32401 agtgtcttcg aatttgaagc ggcgagcgag agtgatctcg ctcgccattg gcacaaggcc
    32461 agaagaatta cttcttcttg gctttcttct tggccttttt cttggctttc gccatggctc
    32521 atctccagta gttacgggtt agtccgaggg cgagtatacg caggcgttga caaatttcaa
    32581 gcgagggtca ctgaacgacg acgatcgaat cacatcgaca cgtatcgcga tgatcattcc
    32641 atcgagagga tgaacttcca atgagcggca ctcagaggcg tgatcgagag tctgttgccg
    32701 cgtttcaaca gcagcatccc atctagttct cgctgctcat gcttgcgcag ttcatcgagt
    32761 gtgatcacgc gcttcaagcg tcgtttgagt tgcacgtcga caacaagcca gcgcggattt
    32821 tttggatcac ttccctcgtc gtagtggtcg tgcttcttgt cgaaggccgt cggatcggga
    32881 tacgcggtgc ggaccacctc gacgatgccc gcaatgccgg gtacgtcgca gctcgagtga
    32941 tagaggaacg cctggtcccc cttcttgaag tcgtcacgca gcatgtttcg ggcctggaag
    33001 ttccgcacgc catcccacat ggccgttttc ttcggacgcc tggcgagatc atcgaccccg
    33061 aaggtcgacg gttcggtctt catgagccaa tagttcatcg atacccctct ccgtgacgat
    33121 ggtgtccatc atgacgtcgg tcgcggtcac gggaaccagc ggcacgacct ggaaagaata
    33181 gacgagaccc accaggcgtg gacctcgcca atgctggcgg aactgccgga agccgagcgt
    33241 gcgatcgtag aagccgaggc cgtgcccgag ccgcgcgccg cggcggtcga gagcgaccgc
    33301 gggtacgaag atggtgtcga gaaaacgggc gctgaggtat tcgacgccgt cgggttcgtg
    33361 cattccgagc gcatggcggg taccaccatg gctgaatctc acgaagcgca tcatgcggct
    33421 gcgcagcgaa gtgatgcggg gcagataaac gtcgcaacca cgggtgagag cgagctggat
    33481 caacgggccc gtgccgatct cctgcggcat cgatgcatag acgccgatgc gtttgccggg
    33541 tctgagccaa tgagtgtggg cgaggatgct ggcgatggaa cgcgcggcgg tcgtccgcgc
    33601 ggcgcgcgga acagcgcggc ggcgctgaag catttcgaag cggagggagg acttcgtttg
    33661 cacaagaaga aaggaaaagc gctacccgat agtgccggtg caagccattg ctctcgaacc
    33721 agagcaacaa ggtgggaccg gccctgacat ttaaggcttt ccgccagagg cggacctgca
    33781 caatgctgtc agaaagcgtt ggtcccgggc gtttacatta cggtcgagag gtaacccgac
    33841 ttactgtcga acaccacagg cagcgccaga acactctacc atcctcaggc gcaagcgcca
    33901 cgtcggaaaa cgccgccaat gaggcggcgt tcgtgaagtg gttggggatt tcgctcaaag
    33961 atccagttgg cgggttcttt gaagcgcacc ttcgacacgt tcgcgcatct gcttcacacg
    34021 gccggtgaca ttgccttgca gatcgtggtc gagtttgcga agacgcagaa gttcattggc
    34081 catgttgagc gcggccatca ccgcgatgcg atctgcaccc accacttttc cggtgtcgcg
    34141 gatctcgcgc atcttcttgt tcaggtattc ggcggagtcg agaaggtcgg cgcgttcgtc
    34201 ctgcgggcag gagacgaaat attccttttc caggatccgt acggtgacgg tcgccggttc
    34261 tttctcgctc atgcagtgtg ctccatcgtt ttcaggcggc cgatcatggc ctccacgcgg
    34321 gcgcgcacct ggtcgttgcg ctgcgcgaac gcggagcgtt cggcggcgag cgattcctgg
    34381 cgctgtcgca aggcgcggtt ctcttctttg agagtgtcga cggtggtgag aagttcttcg
    34441 acgcggcgtt caagcagcgc gatttccgtg gcgaacacgc ttttgccggt cttgttgtta
    34501 tcagatgtgt cgttcatggg cggggaatat agggcacccg gtgccttacc tccaatatat
    34561 tcaggcgcga gcgccactaa ggaaaccgcc gccgaagcgg cgtcgaacat ggatatttgg
    34621 aggttgctct cgcggggctc gggcataatt cccccatgtt gcccgccact tacgatgagt
    34681 tcgaacgcgt gctccgggat gcccgagcat tgccggagcc ggccgaggcg cacggaacgc
    34741 tggccggcgc gctctgcagt tcgcgcgatt acggcctgat cgaatggctg cgcgagatcc
    34801 ttcccgagga ctcacccgag gatgcagccc tgcaaagctc cgttctccag aacgtctaca
    34861 actccatggt gaacaccctg gtcggaagcg atgcggattt cgagccgctg ttgcccgatg
    34921 atgattcacc gctcacgctg cgcgccgatg cactgagcca gtggtgtcag ggctttctat
    34981 acggcctcgg ttccggcacc accagcgatc caggcaaggt gtcgatagag gccggcgaaa
    35041 tcattcgcga tttcaccgag atcactcacg tgggcgtcga cgcggacgaa gagaacgaag
    35101 aaaacgaagt cgccttcgcc gaagtggtcg agttcgtgcg cgtgggtgtg cagttgttgt
    35161 tcgcagagtt ggcgcccgcc cgtggcgagg agccggagcc cggtgccgcg tccatccatt
    35221 agcaagctca cgccccgaag acctgcgccg agcctggcgc gcgtgccttc cgtcgggcgc
    35281 gaagaattca aacgccgccg ccgcgccttg ctcaagcaga tgggccggga ttcgatcgcg
    35341 atcgtgccca cctcacccgt gcgtatgcgc aacaacgacg ttgaatacgc gtaccgtccc
    35401 gacagcgatt ttttctacct caccggtttc gtagagcctg aatcggtggc ggtgctggtg
    35461 ccgggccgcc cgcagggcga atacattctt ttcgtgcggg atcgc
//
LOCUS       DASQJS010000156         9806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3854402, whole genome shotgun sequence.
ACCESSION   DASQJS010000156 DASQJS010000000
VERSION     DASQJS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9806
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3854402"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(530..1207)
                     /locus_tag="VEW08_06555"
     CDS             complement(530..1207)
                     /locus_tag="VEW08_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ40429.1"
                     /translation="MKILVADDDLDLLGLVAYSLSQTGYLVVKASDGHAAISTFDAEA
                     PDLVILDINMPGASGFEVCTAIRARGDVPVMMLTARGEEQDLVKALELGADDYLTKPF
                     SPRTLLARVKALLRRASADRAHAPLSAGRVALDLEGYAVRIGDGGPISLTKLELRLMQ
                     MLLAQAGRAVNSDRLLVQVWGHRGSGDRQLLKQLVHRLRHKIEANPAVPQLLQTAAPS
                     GYKLVVD"
     gene            complement(1204..3264)
                     /locus_tag="VEW08_06560"
     CDS             complement(1204..3264)
                     /locus_tag="VEW08_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ40430.1"
                     /translation="MKRNSLGWWLAASNVAIVLLVAAGISYFAIDMLRGLADSQQKIR
                     VQLAGATARQEITRMAEDTLTYARVLAERPPIGRLLAEPGREGLVPFLRRFCETSELD
                     ACAVFDGAQLVASSGVTLPWPLIFTVSREQGERFMAVPAGTQFSVVGAVAKLPGQYSS
                     FSVMVVHLLDDSVAAVLAEHSGFETRLINYRAFNALPDNEFTSLHSQAMTDGRFAVQR
                     IDSLGLYASSFPVFSSSGEGIALIETRASARETDESVSKLVWRLIVTAFVFAILALVA
                     GVLLGWRVTRPAEALTDAAVRLGQGDFATSIPTAGPAEIGQLARTMDDMRRNLVELNS
                     ELRHREAEAQVVLEAVVEGVYAVDQERNIRYLNPQAARLLGVDTQDAIGRFCGDVLKP
                     FGPNGTRPCTTTACPILRARQAGSAQAVEQLQTSSGTRQTVITSSRVVNGLQVQVLRD
                     ETELEGVRRARDTVLANVSHEFRTPLAAQLASIELLREGLKTSPPEKLDELVLSLERG
                     TLRLTRLIDNLLESVRIESGQLDVRRQSIELADVVGDARALVEALLRQRRQSLEVSVP
                     AGLELQGDGTRLTQVFVNLIANASKFAPEDTAVRVGARADGNQISAWVEDAGPGLPDG
                     DAVSIFERFKRGGSQEPEPGGLGLGLWISRSIVERHGGSITAARTDDGYTRFTITLPT
                     EAAA"
     gene            complement(3261..4136)
                     /gene="cysW"
                     /locus_tag="VEW08_06565"
     CDS             complement(3261..4136)
                     /gene="cysW"
                     /locus_tag="VEW08_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HYJ40431.1"
                     /translation="MGGPSSHADVAAELATRPGHTNLRSTLVRWLLITVSLTFLGALL
                     AAPLAAVFAMALEQGWAAYFSSFSDEATRASIRLTLTTAAIVVPLNTVFGITAAWCIA
                     KFEFRGKSILITLIDLPLAVSPVVSGLIYVLLFGLNGWFGAWLQDHDISVVYALPGIV
                     LATMFVTFPYVARELIPLMQQLGNDEEEAAITLGAGGWFTFFRVTLPRIRWGLIYGVI
                     LCNARAMGEFGAVSVVSGNVRNITMTMPLQIEALYNEYNMVGAFAVASVLSLLAVATL
                     IIKTLVEGRAGYRRR"
     gene            complement(4139..4981)
                     /gene="cysT"
                     /locus_tag="VEW08_06570"
     CDS             complement(4139..4981)
                     /gene="cysT"
                     /locus_tag="VEW08_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HYJ40432.1"
                     /translation="MKNVGRREPNVIPGFGITLGFTTFFLSAIVLLPLSALVLKAAGL
                     DWTRFVEVIASPRALASYRLSFGASLVAASVNMVFGFALAWTLVRYEFPGRKLVDAII
                     DMPFALPTAVSGIALATIFSVNGWIGQYLEPRGIFVAYTPLGITVALTLIGMPFVVRT
                     VQPALQEVQRDLEDAAETLGAGRFYVFRRIIVPTVLPALLTGFTLAFARAVGEYGSVI
                     FIAGNKYMQTEITPLLIIFRLEEYDYAGAAALGFVMLAISFVMLLAINLLQVWGRKRM
                     MVSR"
     gene            complement(4978..6765)
                     /locus_tag="VEW08_06575"
     CDS             complement(4978..6765)
                     /locus_tag="VEW08_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ40433.1"
                     /translation="MSIALDQVTKRYQDQPVVNDVSIEVGEGEFFVLLGPSGSGKSTL
                     LRAIAGLTGIDHGRISLHGRDVTHVRARDRGVGLVFQNYALFRHMTVADNIEFALRVR
                     RMKAADRRERRKELLKLVALEGMDERLPGQLSGGQQQRVAVARALAHKPQVLLLDEPF
                     GALDAKIREELRRTIRAVQRELGITTVLVTHDQEEAFALADTIGVMNHGRLLEVGNPN
                     DLYAQPATRFVATFLGAANLLLARQGADGIRFGTTPVNAEGAEPVQAGREHEVVAVLR
                     PEEVEIAGTREELTSNYIARASVEEIVFTGALERMRVRLLDEPSAAQLLGPSGNGDAR
                     LEITRTQHERRSLPLAQGAHIAIGARRLHVLPTPLSSFLACGATEADAAALAAEPLLE
                     SLSTRMKTRVGLRAIDGLTGTPAPFAGVAAISGGGNAAATVEWLLQHGASEVLVLPRN
                     APSPTRVFIHWTDEGARRATLAVAASLLRHVSAEAVYLGIVPEGRPGSARPAPMRALL
                     DARSEAHQTHGLEMRTELAIGETVTELTRNLSTGSAQMLVLGITDVNQAGSAYRSLLA
                     AQPGWPVLIVYRPVETRTAPELVASGTAA"
     gene            6941..7816
                     /locus_tag="VEW08_06580"
     CDS             6941..7816
                     /locus_tag="VEW08_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYJ40434.1"
                     /translation="MATENAPLNLERTAADKRRERPHWVLDDAVYSISALLLSVIIIH
                     SIWTLIIRPRAEAVLSQRVTVTQTGTNVKAQVLQLRSIYVILKDHEQEICIILCLWSL
                     LLLARQAQDTRLARQLLDRDYVKIGDGQVVLPEDARVYSRPLESLQAAEASRFLPRLL
                     MVAYNRFGATRSVQDAAEAVRDECDFEVASMDTKLSMVRFTAWAIPAVGFVGTVRGIG
                     AALQEAQGAMGGDISGVTMGLGVTFNATLVALVSCIIVMFWMHQLQQFQDRLVLDCRT
                     YVDRQLLRKLRVVSK"
     gene            7849..8208
                     /locus_tag="VEW08_06585"
     CDS             7849..8208
                     /locus_tag="VEW08_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40435.1"
                     /translation="MKTWRSIRIRSFVLPLLLGALALRFLIPPGFMPGGDEGITLQVA
                     MCSTESKNESIEIPGEPAKPHCDHCLMPALDAPLSPVNIAGPIQFPQRLLPWQQESQI
                     PEAPLARAQIPRAPPRA"
     gene            8291..8746
                     /locus_tag="VEW08_06590"
     CDS             8291..8746
                     /locus_tag="VEW08_06590"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HYJ40436.1"
                     /translation="MLRLLLTMLAASALAAAAHAQQFSVGNLVIANPWSRATVSGMLT
                     GAAYLSITNKGSQQDTLFAASTPVAARVEFHRTSLEGGMARMRPAAALVIGPNATVTA
                     EPGGLHLMLVDLKSPLVAGTSIPLVLQFELAGKVTVQLKVEPQGFAPHH"
     gene            8763..>9806
                     /locus_tag="VEW08_06595"
     CDS             8763..>9806
                     /locus_tag="VEW08_06595"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYJ40437.1"
                     /translation="MKQHGRCLLLFAITTSLAAFADDEPARSLGTVTVTATRPSSLPT
                     QIPTTIEGITGGEIAEIINATDSEDALKYFPSLLVRKRYVGDYDHAVLASRASGTGNS
                     ARSLVYADGILLSNLLGNGATFTPRWGLVTPEEIERVDVLYGPFSAAYSGNSVGAVVD
                     YVTRMPEKLEMHAKVASFGQDFGIYDSHGRFTGWQASASAGDRTGNFSWWLNFNHLDS
                     DGQPLSFANRLVSSGVAGTAGTPVTGALADRNPRNQDWLILGSTTQIHTVQEHGKAKL
                     SYDFSPALRASYTLGVWHNDAVRASDSYLRDAAGNPVYSGTINVDGRSYAVTAADFAP
                     GRGELTHVMHGLSL"
BASE COUNT         1767 a         3246 c         3152 g         1641 t
ORIGIN      
        1 tggaaagtca gcgagagtcc gaatgaaacg gagaggcggc ttcgtgccta acgaggccag
       61 gtatcccggc ccgctctctc agctcctgtg cgcggactgc tcgctggggc tcatacgtca
      121 ccgaaccgcg ccccgctttc ccgcggcggt ggtaagagaa gagacgcacg gacttgaggt
      181 cttcccacag cgtggggtta cacgcctctt tttctgacga acggagtcgc ggcgcgtcgg
      241 tgcacgatcg agcgacgggt ggccctgcat gcgatgagcg agcgggccgg gatacctggc
      301 ctcgttaggc acgaagccga tctccgcgtc acacggactc cttgctgccg caccactcaa
      361 ccaagaacaa gatgtcatcg tgtgtgtcgg cggccagcaa actggcgatg tcacaccgaa
      421 cgaagggccc ggcgaaaagc gagtctcatc gcatgcaggg ccgcccgtcg ctcgaccctc
      481 cacatacgag ccacgtcccc tcttccaagc aaaagaggaa tgcccccatt tagtcgacga
      541 cgagcttgta tccggaagga gccgcggtct gcagcaactg cggcacggcg gggttggctt
      601 cgatcttgtg gcgcaaccgg tgcacgagct gcttgagcag ctgccggtcg cccgaaccac
      661 ggtgacccca gacctgcacc agcaggcggt cggaattcac ggcacgaccg gcctgcgcca
      721 gcagcatctg catgagccgc aactcgagct tggtcaacga aatcggtcca ccgtcgccga
      781 tacgcaccgc gtacccctcg agatcgagcg cgacgcggcc ggcggacagt ggcgcgtgcg
      841 cacgatcggc actggcgcgg cgcagtagcg ccttgacgcg cgccagcaag gttcgcggac
      901 tgaacggttt cgtcaggtaa tcgtcggcgc cgagctcgag cgccttcacc aggtcctgct
      961 cttcgccgcg agccgtgagc atcatcacgg gcacgtcacc gcgcgcgcga atggcggtgc
     1021 acacctcaaa accggaagcc ccaggcatgt tgatgtcgag gatcacgaga tccggcgcct
     1081 cggcatcgaa cgtggagatc gccgcgtgac catcgctggc cttcaccacc aggtagcctg
     1141 tttgggacag cgaatacgcc accaggccta acaggtcgag gtcgtcgtcg gccaccagga
     1201 tcttcatgca gcagcctcgg tgggcagcgt gatcgtgaag cgcgtgtagc cgtcgtcggt
     1261 ccgcgccgcg gtgatgctgc ccccgtgacg ttccacaatg gaacgcgaga tccagagtcc
     1321 cagccccagg ccacctggct cgggttcctg cgaaccgccc cgcttgaagc gttcgaagat
     1381 gctcacggcg tcaccgtccg gaagtcccgg cccggcgtcc tcgacccagg cacttatctg
     1441 gttgccatcg gcgcgtgcgc cgacacgcac cgccgtgtcc tcgggtgcga atttgctggc
     1501 attggcgatg aggttgacga atacctgcgt gagccgcgta ccgtcgccct gcagctccag
     1561 ccccgccggt acgctgacct cgagcgactg gcgccgctgg cgcagcagtg cttcgaccag
     1621 cgcgcgcgcg tcacccacca cgtcggcgag ttcgatgctc tgccggcgca catcgagctg
     1681 gcccgattcg atgcgcacgc tctcgagcag gttgtcgatg agccgcgtga gtcgcagcgt
     1741 gccgcgttcc agcgacagga ccagctcgtc cagtttctcg ggcgggctcg tcttcagtcc
     1801 ttcgcgcaac agttcgatgg atgcgagctg cgccgcgagc ggcgtgcgga attcgtgcga
     1861 tacgttcgcg agcaccgtgt cgcgtgcacg gcgcacgccc tcgagttcgg tctcgtcgcg
     1921 caacacctgc acctgcagcc cgttgaccac gcgcgaactg gtgatgacgg tctggcgcgt
     1981 gccggagctg gtctgcagct gttccactgc ctgcgcactg ccagcctggc gcgcgcgcag
     2041 gatggggcac gcggtggtcg tgcaggggcg cgtgccgttg ggcccaaagg gtttgagcac
     2101 atctccgcag aacctgccga tcgcatcctg ggtatcgacg cctaacaatc gcgcggcctg
     2161 cggattgagg taacggatgt tgcgttcctg gtcgaccgcg tacacgccct cgaccacggc
     2221 ttcgagtacg acctgcgcct cggcttcccg atgccgcaat tcggagttga gctcgacgag
     2281 gttgcgacgc atgtcgtcca tggtgcgtgc cagttgcccg atctcggccg gcccggcggt
     2341 gggaatggaa gtggcgaagt cgccctgccc gaggcgcacc gcggcgtcgg tcagcgcctc
     2401 ggccggccgc gtcacgcgcc atcccaagag gacacccgca acgagcgcca atatcgcgaa
     2461 cacgaaggcg gtgacgatca gccgccacac cagtttgctc accgactcgt cggtttcccg
     2521 cgccgaagcg cgggtctcga tgagcgcgat gccttcgccg ctcgaggaga acaccggaaa
     2581 actggaggcg tacaggccaa ggctgtcgat gcgctgcacg gcgaagcgac cgtcggtcat
     2641 cgcctgcgaa tgcagcgagg tgaattcatt gtcaggcagg gcattgaatg cgcggtaatt
     2701 gatgagccgg gtctcgaaac cgctgtgctc ggcgagtacc gccgccaccg agtcatcgag
     2761 caggtgcacc accatcacgc tgaacgagct gtactgcccc ggtagtttgg ctactgcgcc
     2821 gaccacggaa aactgcgtgc cggccggcac cgccatgaag cgttcgccct gctcccggga
     2881 taccgtgaag atcaggggcc atggaagtgt cacgccgctc gacgccacca actgcgcgcc
     2941 gtcgaatacg gcacaggcat cgagctcgga ggtctcgcag aatcgccgca ggaaaggcac
     3001 cagcccctca cgcccgggtt cggccagcag gcgtccgatc ggcggtcgtt cggcgagtac
     3061 gcgcgcgtag gtaagcgtgt cctcggccat gcgcgtgatt tcctggcgcg cggtggcgcc
     3121 ggcgagctgc acgcggattt tctgctggct gtcggcaagg ccgcgcagca tgtcgatggc
     3181 gaaataggaa atacccgccg ccaccagcag cacgatggcc acgttgctgg ccgccagcca
     3241 ccagccgagg ctgttgcgct tcatcggcgc cgataccccg cgcgtccttc gaccagggtc
     3301 ttgatgatca gcgtcgcgac ggcgagcagc gacagcaccg acgccaccgc gaatgcgccc
     3361 accatgttgt actcgttgta cagcgcttcg atttgcagag gcattgtcat ggtgatgttg
     3421 cgaacgttgc cggagaccac ggacacggcg ccgaactcgc ccatcgcccg cgcgttgcac
     3481 aggatgacgc cataaatgag gccccagcga atgcgcggca gcgtaacacg gaagaacgtg
     3541 aaccagccgc cggcccccaa cgtgatcgcg gcttcttcct cgtcgttgcc gagctgctgc
     3601 atgagcggaa tgagctcgcg cgccacgtac gggaacgtga cgaacatcgt cgcgaggacg
     3661 atgcccggca gcgcgtagac gacgctgatg tcgtggtcct gcagccaggc gccgaaccag
     3721 ccgttcaggc cgaacagcaa tacatagatg agacccgaca ccaccggcga caccgccagc
     3781 ggaagatcga tgagcgtgat gaggatgctc ttgccgcgaa attcgaactt ggcgatacac
     3841 caggcggcgg tgatgccgaa cacggtattg agcggaacga caatagccgc agtggtcagc
     3901 gtcaggcgga tggaggcacg cgtggcttcg tcgctgaaac tcgagaaata cgcggcccag
     3961 ccctgctcca gggccatggc gaacaccgcg gccagcggcg cggccagcag tgcgcccagg
     4021 aaggtgagcg agacagtgat gagcagccag cgcaccagcg tagagcgcag attcgtgtgc
     4081 ccgggccggg tagctagttc ggcggcgacg tcggcgtgtg aggaaggacc gcccatgact
     4141 aacgactcac catcattcgc ttgcggcccc agacctgcag caggttgatg gccagcaaca
     4201 tcacgaaaga aatcgccagc atcacgaaac cgagcgcggc ggctcccgcg taatcgtatt
     4261 cctcgaggcg gaatatgatg agcagcgggg tgatctcggt ctgcatgtac ttgttgccgg
     4321 cgatgaagat gaccgagccg tattcaccca cggctcgcgc gaatgccagc gtgaatccgg
     4381 tgagcagtgc cggcaatacc gtgggcacga tgattcgccg gaacacgtag aagcggccgg
     4441 cgccgagcgt ctcggcagcg tcttcgagat cgcgctgcac ttcctgcagg gccggctgca
     4501 cggtgcgcac cacgaagggc atacctatga gagtgagtgc gacggtgatg ccgagcggcg
     4561 tatacgcgac gaagattccg cgcggctcga gatactggcc gatccagccg ttgaccgaaa
     4621 aaatggtggc cagggcgatg cccgatacgg cggtgggcag tgcgaacggc atgtcgatga
     4681 tcgcatcgac cagtttgcgg cccggaaact cgtaacgcac cagcgtccac gccagggcga
     4741 aaccgaagac catgttcacg ctggccgcga ccagtgacgc gccgaacgac agcctgtatg
     4801 acgccagcgc gcgcgggctg gcgatcacct cgacgaaacg cgtccagtcg agacccgcgg
     4861 ccttgagcac cagcgccgac aacggcagca gcacgatggc cgacagaaag aacgtggtga
     4921 accccagtgt gatcccgaag ccgggaatga cgttgggttc gcgccggccg acatttttca
     4981 tgccgcggtt cccgatgcga cgagttcagg tgctgtgcgt gtttccaccg gacggtagac
     5041 gatgagcaca ggccagcccg gctgtgcggc taacaagctg cggtacgcgc tgcctgcctg
     5101 gttgacgtcg gtgatgccga gcaccagcat ctgcgcactg cccgtggaca ggttgcgcgt
     5161 gagctcggtg acggtctcac cgatggcaag ttccgtgcgc atctcgaggc cgtgagtctg
     5221 atgcgcttcg gagcgcgcgt cgagcagcgc acgcatgggc gcggggcggg cgcttccggg
     5281 acgaccttcg ggcacgatgc ccaggtacac cgcttccgcc gacacgtgac gcagcaggct
     5341 ggccgccacg gcaagcgtcg cgcggcgggc gccttcgtcg gtccagtgga tgaacacgcg
     5401 ggtcgggctc ggagcattgc gcggcaggac caggacctcc gaggcgccgt gctgcaacag
     5461 ccattcgacg gtcgccgcag cgttgccgcc gccggaaatg gcggccacac cggcgaatgg
     5521 cgccggcgtg ccggtgagcc catctatggc gcgcaggccg acgcgcgtct tcatgcgcgt
     5581 ggaaaggctc tcgagcaaag gttccgccgc cagtgccgcc gcgtcggcct cggtggctcc
     5641 gcaggcgagg aagctggaca aaggcgtggg cagcacgtgc agccggcgcg caccgattgc
     5701 aatatgcgcg ccctgcgcca gcggcaggct gcgacgctcg tgctgggtgc gcgtgatttc
     5761 gagccgcgca tcgccattgc ccgaggggcc caacagctgt gccgcactgg gttcatccag
     5821 aaggcgcacg cgcattcgtt cgagcgcgcc ggtgaagacg atttcctcca cggaagcgcg
     5881 cgcgatgtag ttggaggtca gttcttcgcg cgtcccggcg atttcgactt cttcggggcg
     5941 caggaccgcc accacttcgt gctcgcgacc ggcctgcacg ggctcggcgc cttcggcatt
     6001 caccggcgtc gtaccgaagc ggatgccgtc tgcgccctgg cgggccagca gcagattggc
     6061 ggcgccaagg aaagtcgcca cgaagcgcgt ggcgggttgc gcgtacaggt cgttcggatt
     6121 gcccacttcg agcaggcggc cgtggttcat gacgccgatg gtgtcggcca gcgcgaatgc
     6181 ctcttcctgg tcgtgcgtca cgaggacggt ggtgatgccc aactcgcgct gcacggcgcg
     6241 gatggtccgg cggagttctt cgcggatctt ggcgtcgaga gcgccgaagg gttcatcgag
     6301 caacagcacc tgcggcttgt gcgccagcgc gcgcgccacc gcgactcgtt gctgctgacc
     6361 gcccgagagc tgtcccggca gacgctcgtc catgccctcg agcgccacca gcttgagcag
     6421 ttccttgcga cgctcgcggc ggtcggcggc cttcatccgg cgcacgcgca gtgcaaattc
     6481 gatgttgtcc gccaccgtca tgtggcggaa cagcgcgtag ttctggaaca ccagtccgac
     6541 gcctcggtcg cgcgcccgca cgtgggtgac gtcgcggcca tgcagggaga tacgcccgtg
     6601 gtcgatgccg gtgaggccgg caatggcccg aagcagggtg ctcttgccgc tgcccgaggg
     6661 acccagcaac acgaagaact cgccctcgcc cacctctatg gaaacgtcgt tgacgacggg
     6721 ctgatcctga taccgtttgg tgacttggtc gagtgcgatg gacatgagtg gaaggcgggc
     6781 tgaaaaccgg cggtgatgct atcagcatgc cggtatccgc ccgaatatcg cggtggttac
     6841 aatgaggtga caaacggttc taaggccgtg gaacttaatc ggttacctcc ccgactaacg
     6901 agtagacttt ttgccctctt acccgcagcg agaaacctga atggccaccg agaacgcgcc
     6961 tctcaacctg gaaagaaccg ccgcggataa gcgtcgcgaa cggccgcact gggttctgga
     7021 cgacgcggtc tacagcatca gcgccctgct gctctcggtc atcatcatcc acagcatctg
     7081 gacgctcatc atccgcccgc gcgccgaggc cgtgttgtca cagcgggtca ccgtcaccca
     7141 gaccggcacc aacgtgaagg cccaggttct gcagctgcgg tcgatctacg tcattctcaa
     7201 agaccacgag caggagatct gcatcatcct gtgcctgtgg tcgctgctgc tgctcgcccg
     7261 tcaggcgcag gacacgcgac tcgcccgcca gctgctcgat cgcgactacg tgaagatcgg
     7321 cgacggccag gtggtgctgc ccgaagacgc gcgcgtctac tcgcggccgc tcgaatccct
     7381 gcaggccgcc gaagcgagcc gcttcctgcc gcgccttctc atggtggcgt acaaccgctt
     7441 cggcgccacg cgcagcgtgc aggacgccgc cgaggcggtg cgcgacgaat gcgacttcga
     7501 agtcgccagc atggacacca agttatccat ggtccgtttt acggcgtggg caattccggc
     7561 cgtgggcttc gtcggcacgg tgcgcggcat cggcgcggca ctgcaggagg cgcaaggcgc
     7621 catgggcggc gacatctccg gtgtcaccat gggcctgggc gtcacgttca acgctactct
     7681 ggtcgctctt gtgtcctgca tcatcgtcat gttctggatg caccagctgc agcagttcca
     7741 ggatcgcctg gtgctcgact gccgcaccta cgtcgatcgc cagctgctac gtaaactccg
     7801 cgtcgtcagc aagtagcaaa ccccggcgaa ggggttaaaa tgcgccgcgt gaagacttgg
     7861 cgctcgatcc gcattcgctc ttttgtgctg ccgctgctgc tcggcgcgct tgcactgcgt
     7921 tttctgatcc cacccggctt catgcccggc ggagatgaag gaatcacgct gcaggtggcc
     7981 atgtgttcca ccgaaagcaa gaacgaatcc atcgaaatcc ccggcgagcc ggcgaagccc
     8041 cattgcgatc attgcctgat gccggcgctg gacgctccgc tctcgcccgt caacatcgcg
     8101 ggtccaatcc agtttccgca gcgcctgcta ccgtggcagc aggaatccca gatccccgaa
     8161 gcgccgctcg cccgcgcgca gattcctcga gcccctcccc gcgcctgacc gctccggttt
     8221 cgcctgagga accgcccgtc agtttcatgc gtgctcttgc acgcgtgtgt tcacgcgatc
     8281 gaggattcac atgctccgtc tcctgctcac aatgctggcg gccagtgcgc tggccgctgc
     8341 cgcgcacgct cagcaatttt cggtcggtaa cctggtcatc gccaatccgt ggtcgagagc
     8401 cacggtctcc ggcatgctga ccggcgccgc ctatctatcc atcaccaaca agggctcgca
     8461 gcaggacacg ctgttcgcgg caagcacgcc cgtcgcggca cgcgtcgaat ttcatcgcac
     8521 cagtctcgaa ggaggcatgg cgcgcatgcg gcccgccgcc gccctggtca tcggccccaa
     8581 cgccaccgtc accgccgaac cgggcggcct gcacctgatg ctggtcgacc tgaaatcgcc
     8641 gctggtggcg ggcacatcca ttccgctggt gctgcagttc gagctggccg ggaaagtcac
     8701 cgtgcaattg aaggtcgaac cgcaagggtt cgcgccacac cactgaagcg cggggaatac
     8761 tcatgaaaca gcatggacgc tgcctgctcc tcttcgcgat cacgacctca ctcgcggcat
     8821 tcgccgatga cgaaccggcc cgctcgctcg gcaccgtgac cgtcacggcc acgcggccga
     8881 gctcactgcc cactcagata cccaccacca tcgagggcat caccgggggc gagatcgccg
     8941 aaatcatcaa tgccaccgac agtgaagacg cgctgaagta cttccctagc ctgctggtgc
     9001 gcaagcgcta cgtcggcgac tacgaccacg ccgtgctcgc cagccgcgct tcgggcaccg
     9061 gcaatagcgc ccgctctttg gtgtatgccg acggcatcct gttgtcgaac ttgttaggca
     9121 atggcgccac gttcacaccg cggtggggcc tggtgacacc cgaggagatc gagcgggtcg
     9181 acgtgctcta cggcccgttc tccgccgcgt attccggcaa ctccgtgggg gccgtggtcg
     9241 actacgtcac gcgcatgcca gaaaagctcg agatgcacgc gaaggtcgcg agctttggcc
     9301 aggatttcgg gatttacgac agccatggtc gattcaccgg ctggcaggcg agcgcctcgg
     9361 cgggggatcg caccgggaac ttttcctggt ggctcaactt caaccatctc gacagtgacg
     9421 ggcaaccgct gtcattcgcc aacagactgg tgagcagcgg cgtcgcgggc acggccggca
     9481 cacccgtcac cggcgcactc gcggaccgca atccacgcaa ccaggactgg ctcattctcg
     9541 gttcgacaac ccagattcac acggttcagg aacacggcaa ggcgaaactg agttacgact
     9601 tctcgcccgc gctgcgtgcg agttacacgc tcggcgtgtg gcacaacgat gccgttcgcg
     9661 cctccgactc ctatctgcgc gatgccgccg gcaacccggt ctacagcgga accatcaatg
     9721 ttgacggccg ttcctatgcg gtgaccgcgg cggatttcgc gccgggccgt ggcgaactca
     9781 cgcacgtgat gcacgggctg tctctc
//
LOCUS       DASQJS010000157         8720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3229751, whole genome shotgun sequence.
ACCESSION   DASQJS010000157 DASQJS010000000
VERSION     DASQJS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3229751"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VEW08_06600"
     CDS             complement(<1..418)
                     /locus_tag="VEW08_06600"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYJ40438.1"
                     /translation="MRVFYLLLAVLVTACSGNDASSASTKPGAVRIGNAFTATPVADF
                     QEPWAMAFLPDGRLLVTEKKGALKLLDVNSKRVGKISGVPEVAYGGQGGFGDVILHPQ
                     YATNSIVYVSYSEPGDGPGESNVNGAAVARARLQLDR"
     gene            complement(418..1140)
                     /locus_tag="VEW08_06605"
     CDS             complement(418..1140)
                     /locus_tag="VEW08_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HYJ40439.1"
                     /translation="MKKLTVIVLATLSVAYAGYVDAATPKKRTRSSNRIGAYGGVQVG
                     YSSFAAENSAADEEGLENTLINSGAITIENLRSSSEDTDIGYQAAFGYRFHRYFAAEL
                     GLAQFGEMSSTASADMDFDDGAGVVPVSIKVSYRAGGPIMSAIGILPLHEKFELFARL
                     GYMFTSSEREFSARIDGQSGGSGSAKGDSQDPVYGVGFAWHINQVYSIRGEFQKLDSL
                     GQENRTGEEDLSVFGLGLVIRF"
     gene            complement(1201..1911)
                     /locus_tag="VEW08_06610"
     CDS             complement(1201..1911)
                     /locus_tag="VEW08_06610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYJ40440.1"
                     /translation="MREQHIEYRDGEALLEGFLCYDEAQPGPRPTVLISHAWAGRNDF
                     VERKARRLAWQGYACFALDNYGKGKRGTTPEECSALMTPLMKDRKLLLKRLQAGLATA
                     RSMPIVDSRRVAIMGFCFGGLCALDLARSNADIRAAVSFHGMLTPSGFTEPKVRARVL
                     MLHGYDDPLAPPQDLLAVAKEFTEAGADWQLHAYGHTVHAFTNPVAQDRDGGMQYDES
                     ADRRSWHALEEFLAETLK"
     gene            complement(1949..5065)
                     /locus_tag="VEW08_06615"
     CDS             complement(1949..5065)
                     /locus_tag="VEW08_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HYJ40441.1"
                     /translation="MIDSLLQFAIARRWLIIACTLMLAALGVYNYARLPIDAVPDITN
                     VQVQVNAIAAGFTPLEVEQRVTVPIEAAIAGLPHVEYSRSLSRYGLSQVTVIFDEGTD
                     IYFARQMISERLTEAADQLPENVDATLGPVATGLGEIFLYTVHADPDARQANGQPYDS
                     MALRSLQDWVIKPQLRQVRGVAEINSIGGYAHQIHVTPDPLRMLGFGVTFEDIVEALE
                     HDNGNAGAGFIERNGEELLVRATGQAASASDIASVVIADRGGRPVRVADVAGVGIGKE
                     LRTGAATRDGAETVLGTALMLVGENSRTVSQRVARKLAEIEGSLPKGVHTEAVYDRTT
                     LVNATIRTVQANLAEGALLVVVVLLVMLGHVRAALLTALVIPFSMLMLVSGMVTTHVS
                     ANLMSLGALDFGLIVDGAVIIVENCLLRLGQEQQRLGRILALPERLNAVFTATREVVT
                     PSAVSVLVVILVNLPLLALSGVEGKMFHPMALTVIMALMAALLLSLTFVPAACALLLK
                     GRLSHEENRVVAGLRRGYAPALTWAIRHRGAVVVSAIVFTMLGAWGATRLGTEFVPSL
                     DEGDIAVQASRIPGTSLSQSIALQTAVEKAVMTLPEVRTAFSRIGTAEVATDPMPPSI
                     ADGLVMLKPRSEWPDPEKPKDELLEDLDRALKTVPGSNYEISQPIQLRFNELISGVRS
                     DLGIKIYGDDLDVLLRQGSRIAQVLRGVKGASDVKLEQVTGLPMLEVAPRREALARHG
                     VHVGDLQNLVVTAIGGHEAGQVFDGDRRIDVIVRMPEALRSDLRVLARLPVELNRGGY
                     VPLGELADLREVVRPNQVSRENGKRRLVVSANVSGRDLGGFVADARAAISREIKLPAG
                     YWLDYGGTFEQLESASARLALLVPLTLLLIFGLLFATFHSARDALLVFSGVPLALVGG
                     VAALALRGMPLSITAGVGFITLSGVAVLTGVIVVTAFRDLTRAGRSLDEAVVEGALLR
                     LRPVLLIGLVASLGFLPMALNTGTGAEVQRPLATVVIGGILSATLLSLFVLPALYRWA
                     ARPATGVT"
     gene            complement(5067..5963)
                     /locus_tag="VEW08_06620"
     CDS             complement(5067..5963)
                     /locus_tag="VEW08_06620"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ40442.1"
                     /translation="MKQTLLFLLASAMLSACGDSGQRDEHAEAEEHGAAVPAHVATAT
                     VENAGSARIRDIVQAYGTVALNAERTRHVSARFAGVVTAVAKAPGDPVRAGDTLASIE
                     SNESLQVYAVRAPIAGVVMDRTVNAGETVEQQSLFTVSDLSSLWAELLVFPGDVARLR
                     TGQPVSMRSADGALKATATISHIAPAGDRDTQAIVVRVPIDNRAGQWRPGVGISADIA
                     VAEIEVPVSVRSTALQTVDGERVVFVVTEAGYEPRPVVTGRADEEHTEIASGLAAGER
                     YVATDSFVVKAELEKSEAGHEH"
     gene            complement(5960..7228)
                     /locus_tag="VEW08_06625"
     CDS             complement(5960..7228)
                     /locus_tag="VEW08_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYJ40443.1"
                     /translation="MKISKAGAWCASLMCGLASVCATADDGGLTLADAINNALTRNPE
                     LRASAFRISRAQAELEEARLRPAPELALELENFAGSGDLHGTEALESTLSFTRVLELG
                     GKRSARVEAAELAGDLAALDQRARELDILAEVTRRFLAAAAAAEQLSLAKETEHLAQE
                     TLHAIARRVAAARSPLADESRARIAATRANLDVEQATSTSRTRRYALAASWGETEPQF
                     GALRADLFSFESDPGFAALMKRMERNPAFLRFAGEIRFRDAQLRLAQAQNRPDLALTL
                     GLRQLQEGGDTALVAGVSMPLNLPKRNETTLRRARVDLAQAQAEQDAAAVLARTTLFG
                     LYEEMSRSRRRAEALRADALPLARTALEQTQAGYDRGRFAFSELAAARQELLALQSAA
                     IESAATYHELRTEIQRLTGEPLNSIEATQP"
     gene            complement(7288..7683)
                     /locus_tag="VEW08_06630"
     CDS             complement(7288..7683)
                     /locus_tag="VEW08_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40444.1"
                     /translation="MARFGNQSRFLIATLCVAMLVLRIGGLHVHMCMDGTEPPLSFHV
                     EDSGIHHLDETSAAEEHADRDVGLVSEVVLKKPFGGLDLGLVAAFFALILFLLTRQHE
                     LPAFPSLPAKVRSAWNRLRPPLRGPPRLA"
     gene            complement(7731..8561)
                     /locus_tag="VEW08_06635"
     CDS             complement(7731..8561)
                     /locus_tag="VEW08_06635"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HYJ40445.1"
                     /translation="MKKLLVTLLFAAAGVAGVVQPTFASAREEARLIEASGVLEELRA
                     QRDTLIPDRLMARAYGIAVIPNVVKIAVGVGGRRGSGALVVRDVNGRFSNPIMVSITG
                     GNVGWQIGVQSTDIVLVFTTRKSIEGITDGKLTLGGDASVAAGPVGRAASAATDQNFT
                     AEVYSYSRNRGLFAGVSIDGSVIAIDTKSNNKLYGKTAPATDIIAGHITTDADAAKRF
                     QRAILASTSGAQPAIAPHSAATSTPDGAVPAATENELPPSGAQTFPMEDAQPGAEPPR
                     "
BASE COUNT         1510 a         2779 c         2876 g         1555 t
ORIGIN      
        1 cgcgatcgag ctggagtcgg gcacgcgcga ccgcggcgcc attcacattg ctttcgcccg
       61 ggccatcgcc gggttcggaa taactcacgt agacgatgga gttggtcgcg tactgcggat
      121 gcaggatcac gtcgccgaag ccgccctgac cgccataggc gacttcaggc actccgctga
      181 ttttgccgac gcgtttgctg ttcacgtcca gtagtttgag tgcgcctttc ttctccgtga
      241 ccagcaggcg cccgtcggga aggaaggcca tggcccaggg ttcctggaaa tccgccacgg
      301 gcgtggcggt gaaggcattg ccgatgcgca cggcgccagg cttcgtggat gcgctggaag
      361 cgtcgttgcc gctacacgca gttaccagca ccgcaagcag gaggtaaaag actctcatca
      421 gaagcggatc acgagtccca ggccgaatac cgagaggtct tcctcaccgg tgcgattttc
      481 ctggcccagc gagtccagtt tctggaattc accgcggatg gagtaaacct gattgatgtg
      541 ccaggcgaag ccgacgccgt acacgggatc ctgcgagtct cctttcgcgc tgccggagcc
      601 gccggattgg ccgtcgatcc tcgcgctgaa ctcgcgttcg gaactggtga acatgtagcc
      661 gagacgggcg aacagttcga acttctcgtg caagggcagg atgccgatcg ccgacatgat
      721 gggtccgccc gcgcggtatg aaaccttgat gctcaccgga acgacgccgg cgccatcgtc
      781 gaagtccatg tcggcgctcg cggtggagga catctcgccg aattgcgcga gaccgagttc
      841 cgccgcgaag tagcgatgga agcgatagcc gaacgccgcc tggtagccga tatcggtgtc
      901 ttccgagctc gaccgcaaat tctcgatggt gatggcgccg gaattgatca gcgtgttttc
      961 gaggccttct tcgtcggccg cggaattttc ggcagcgaag ctgctgtagc ccacctgcac
     1021 gccgccatag gcgccgatgc ggttggagct gcgcgtgcgt ttcttcggtg tggctgcatc
     1081 gacgtatccg gcgtacgcaa cgctcaaggt tgcgagcacg attaccgtta acttcttcat
     1141 gcagaccgct cccctgggaa gtccgggatc acgctaacac gcgacgaagg aattcgccgc
     1201 tcatttgaga gtttcggcga ggaactcctc gagcgcatgc cacgaacgcc gatctgcgct
     1261 ttcgtcgtat tgcattccac cgtcgcgatc ctgcgccacg ggattggtga atgcgtggac
     1321 ggtgtgacca taggcatgaa gctgccagtc ggcgcctgcc tcggtgaact ccttcgccac
     1381 ggcgagcagg tcttgaggtg gcgccagcgg atcgtcatag ccatgcagca tgagtaccct
     1441 ggcccgaact ttcggttccg tgaacccgga gggtgtcagc atgccgtgaa aactcacggc
     1501 ggcgcgaatg tcggcgttgc tgcgcgcgag atcgagcgcg cacaggccgc cgaaacagaa
     1561 gcccatgatg gccacgcggc gtgaatcgac gatgggcatg gacctggcag tggcgagtcc
     1621 ggcctgcagg cgcttcagca gcagcttccg gtccttcatc aacggagtca tgagcgcgct
     1681 gcattcctcc ggggtcgtgc cgcgtttgcc cttgccgtag ttgtcgagcg cgaaacacgc
     1741 gtagccctgc caggcgagtc gccgtgcctt gcgttccacg aagtcgttcc ggccggccca
     1801 ggcgtggctg atcagcaccg ttggacgcgg ccccggctgc gcttcgtcgt agcagaggaa
     1861 accttcgagc agcgcttcgc cgtcgcggta ttcgatgtgt tgctcacgca tgttcgatcc
     1921 cccactgccg cgtatatata tgaatcgatt atgtaacacc cgtagccggc ctggcagccc
     1981 atcgatacag cgcgggcagc acgaacaagc tgagcaatgt ggccgagagg atcccgccga
     2041 tcaccaccgt ggcgagcgga cgctgcactt cggcgccagt tcccgtgttg agcgccatgg
     2101 gcaggaaacc cagtgaagcg acgagtccga tcaagagtac gggccgcaat ctcaacagtg
     2161 ctccctccac gacggcttcg tcgaggctgc ggcccgcgcg cgtcagatcg cggaacgccg
     2221 taacgacaat gacgcctgtg agaaccgcga ctcccgaaag cgtgatgaag cccacgcccg
     2281 cagtgattga cagcggcatg ccgcgcaatg ccagtgccgc gacacctccc acgagtgcca
     2341 atggcacgcc gctgaatacc agcagcgcat cccgcgccga gtgaaatgtg gcgaacagaa
     2401 gtccgaagat cagcagcaag gtgagcggca cgagcagggc gagccgggcg ctcgccgatt
     2461 cgagttgctc gaaggtgccg ccgtagtcca gccagtaccc ggcaggcagc ttgatctcgc
     2521 gcgagatggc tgcgcgcgca tcggccacga agccgccgag gtcacggccg ctcacattgg
     2581 ccgagaccac gaggcggcgt ttgccatttt cgcggctgac ctgattcggg cgcaccacct
     2641 cgcgcaggtc ggcaagctcg ccgagcggca cataaccacc gcggttcagc tcgactggca
     2701 gtctcgcgag cacgcgcagg tcggatcgca acgcctcggg catgcgtacg atcacatcga
     2761 tgcggcgatc gccgtcgaag acttgtccgg cctcatggcc gccgatggcc gtcaccacga
     2821 gattctgcaa gtcgccgaca tgcacgccgt gtctcgcgag tgcctcgcgc ctgggcgcca
     2881 cttccagcat gggtagtccg gtcacctgct cgagtttcac gtccgacgca cctttgactc
     2941 cacgcaggac ttgcgcgatg cggctgccct ggcgaagcaa cacgtcgaga tcatcgccat
     3001 agatcttgat accgagatcg gagcgcacac cggaaatgag ttcattgaag cggagctgaa
     3061 tgggctggct gatttcgtag ttgctcccgg gcacagtctt gagtgcccga tcgagatcct
     3121 cgagcaattc gtccttgggt ttttccgggt ccggccattc gctgcgcggc ttcagcatga
     3181 cgaggccatc ggcaatgctg ggcggcatcg gatcggtggc gacttcggca gtgccgatgc
     3241 ggctgaacgc cgtgcggact tcgggcagcg tcatcaccgc cttttccacg gccgtctgca
     3301 gcgctatcga ctgactcagg ctcgtgcccg gaatgcggct ggcttgcacg gcgatgtccc
     3361 cttcgtcgag gctcggcacg aactccgttc ccagccgtgt cgcgccccag gcgccgagca
     3421 tcgtgaacac gatcgcacta actacgacgg caccgcgatg gcgaatggcc caggtgagcg
     3481 ccggcgcgta gccgcgccgc agaccggcga cgacgcgatt ttcctcgtgc gaaagcctgc
     3541 ccttgagcag cagtgcgcag gccgccggca cgaacgtcag cgacagcagc agggcggcca
     3601 tcaaggccat gatgaccgtg agtgccatcg ggtgaaacat tttcccttcg acccccgaca
     3661 gcgccagcag cggcagattg acgagtatca cgacgagcac gctcaccgcg ctcggcgtca
     3721 cgacttcgcg ggtcgcggtg aacacggcgt tcaggcgctc gggaagcgcg agtatgcgac
     3781 ccaggcgttg ctgttcctgg ccgagacgca acaggcagtt ctcgacgatg atcaccgcac
     3841 cgtcgacgat gaggccgaaa tcgagcgcgc ccagcgacat gaggtttgcg ctgacgtgcg
     3901 tcgtcaccat gccgctgacc agcatgagca tcgagaaagg aatcaccagc gcggtgagca
     3961 gtgcggcacg cacatgcccg agcatgacca gcaggaccac gacgaccagc aacgcacctt
     4021 cggccaggtt cgcctgcacc gtgcggatgg tggcgttgac gagggtggtg cgatcgtaga
     4081 ccgcttcggt gtgtacgcct ttgggcagcg agccctcgat ctcggcgagc ttgcgggcta
     4141 cgcgctggct gacggtacgg ctgttctcgc cgaccagcat gagcgctgtg ccgagcacgg
     4201 tttccgcacc gtcgcgcgtg gctgccccgg tgcgcaactc cttgccgatc ccgacgccag
     4261 caacgtccgc cacgcgcacc gggcggccgc cgcgatcggc gattaccacg ctggcgatgt
     4321 cactggcgct cgcggcctgg ccggtagcgc gcaccagaag ttcttcaccg ttgcgctcga
     4381 tgaagccggc gcctgcgttg ccattatcgt gttcgagtgc ctcgacgatg tcttcgaagg
     4441 tgacgccgaa gccgagcatg cgcagcggat ccggcgtgac gtggatctgg tgcgcatagc
     4501 cgccgatgga attgatttcg gcaacgccgc gcacctgccg aagctgcggc ttgatcaccc
     4561 agtcctgcag ggaacgcaat gccatgctgt cgtaaggctg gccattggcc tgacgcgcgt
     4621 cggggtcggc gtgaaccgtg tagaggaaga tctctccgag gccggtggct accggcccaa
     4681 gtgtcgcgtc gacattctcc ggcaactgat cggcggcttc ggtgagcctc tcggaaatca
     4741 tctggcgcgc aaagtagata tccgtgccct cgtcgaagat cacggtgacc tgcgacaggc
     4801 cgtaccgtga cagcgaccgc gaatattcca cgtgcggaag accggcgatc gccgcttcga
     4861 ttggcacagt cacacgctgc tcgacttcga gcggcgtgaa gccggcggcg atggcgttga
     4921 cctgcacctg gacgttggtg atgtccggca cggcatcgat gggcaggcgg gcgtagttat
     4981 agacgcccag ggccgccagc atcagcgtgc aggcgatgat gagccaccgg cgggcgatgg
     5041 cgaattgcag cagtgagtcg atcatgtcag tgctcgtgac cggcttctga cttctcgagc
     5101 tcggctttca ccacgaagct gtcggtcgcc acgtaacgct cacccgcggc gagccccgac
     5161 gcgatttcgg tgtgctcttc gtcggcgcgg ccggtcacga cgggacgagg ttcatatccg
     5221 gcttcagtca cgacgaacac cacgcgctcg ccgtccacgg tctgcagcgc cgtgctccgc
     5281 acgctcaccg gcacttcgat ttcggcgacg gcaatatccg cagagattcc aacgccgggg
     5341 cgccactggc cggcgcggtt gtcgatcggg acacgcacga cgatggcctg cgtgtcgcga
     5401 tcgcccgccg gggcaatgtg cgaaatggtc gcggtggcct tgagtgcacc gtcagcggac
     5461 cgcatcgaca cgggctgacc ggtccgcaga cgcgccacat cgcccggaaa aaccagcagc
     5521 tcggcccaca gagaactcag gtccgaaacc gtgaacagcg attgctgctc cacggtctcg
     5581 ccggcgttca cggtgcgatc catcaccacg cccgcaatcg gcgccctcac ggcgtagacc
     5641 tgcaggctct cgttgctttc gatgctggcc agcgtatcgc cggctctcac cgggtcgccc
     5701 ggcgctttcg cgacggcggt taccacgccg gcaaatcgtg ccgacacgtg gcgggttcgt
     5761 tcggcgttca aggcaacagt gccataggcc tgcacgatgt cgcggattcg cgcgctgccg
     5821 gcgttttcga cggtggctgt cgcgacatgg gcaggtactg cggcaccgtg ttcttcggcc
     5881 tctgcatgtt catcgcgctg gcctgagtct ccgcaagcgg aaagcatcgc gctggctaac
     5941 aggaaaagca gggtttgttt catggctggg tcgcctcgat ggagttgagc ggctcgccgg
     6001 tgagtcgttg gatctcggtg cgaagctcgt ggtaggtggc cgccgactcg atggcggcgc
     6061 tttgcagtgc cagcagttcc tggcgggcgg cggcaagttc ggaaaacgcg aatcgcccgc
     6121 ggtcgtagcc ggcctgcgtt tgttcgagcg cggtgcgtgc caggggcagg gcatccgcgc
     6181 gcagcgcttc ggcacgccgc cgcgacctgc tcatttcttc gtagaggcca aacaaggtgg
     6241 tgcgcgccag taccgcggcg gcgtcctgct cggcctgggc ctgcgccagg tccacgcgcg
     6301 cgcggcgcag ggtggtctcg ttgcgcttcg gcaggttgag cggcatggag acgcctgcca
     6361 ccagggcggt gtcgccgcct tcctgcagct gccgaagccc caatgtcagt gccaggtccg
     6421 gacggttttg ggcctgcgca aggcgcaact gcgcatcgcg gaaccggatt tcgccggcga
     6481 agcgcaggaa ggcaggattg cgctccatgc gtttcatgag cgctgcgaag ccggggtcgg
     6541 actcgaacga gaacaggtct gcgcgcagcg caccgaattg cggttcggtt tcaccccagc
     6601 tcgccgccaa cgcgtagcgc cgcgtgcgcg acgtgctggt cgcctgctcg acgtcgaggt
     6661 tggcgcgggt ggcggcgatc cgcgcgcgac tctcatccgc cagcggcgat cgtgccgccg
     6721 cgacgcggcg cgcgatggcg tggagcgttt cctgggcgag gtgttcggtc tccttcgcca
     6781 ggctcaattg ctcggcggcc gcggccgcgg ccaggaatcg ccgcgtcact tcggccagga
     6841 tatcgagctc acgagcgcgt tggtcgagcg ccgcgagatc gcccgccagt tcggcagctt
     6901 ctactcgtgc ggatcgcttg ccaccgagct cgagaaccct ggtgaagctc agggtgcttt
     6961 ccagtgcttc cgtgccgtgc aggtcgccac tgccagcaaa gttttccagt tccagcgcga
     7021 gttcgggcgc gggccgcaat cgggcctctt caagttctgc ctgcgcgcgc gaaatccgga
     7081 atgcactggc ccgcaactcc ggattgcgcg tgagcgcatt gttgatggca tccgccagcg
     7141 tgagcccgcc gtcatcggcg gtagcgcaga cactcgcgag tccgcacatc agactggcgc
     7201 accaggcgcc ggccttcgag attttcatga tccactcccg cgcacgccca tgcgtgcgcc
     7261 gacacaccta actaccgtcg aatcaggtca ggcgaggcgg ggaggtccgc gtagcggcgg
     7321 ccgcaggcga ttccaggcgg agcgcacttt ggcgggcagg gagggaaatg cgggcagctc
     7381 gtgctggcgc gtgagcagga acagtatcag cgcgaaaaac gcggccacca gccccaggtc
     7441 gaggccgcca aagggcttct tgagtactac ctcggacacg aggccgacgt cacgatcggc
     7501 atgttcttcg gcagcgctgg tttcatcgag atgatgaatg ccggagtcct cgacgtggaa
     7561 ggacagcggt ggctcggtgc cgtccatgca catgtggacg tgcaggccgc cgatgcgcaa
     7621 cacgagcatc gccacgcaga gcgtggcgat gaggaaccgg gattggttac cgaagcgagc
     7681 cattcctgca gtatgtacca gagggattgg cgctgaccgg cgaactgggc ctaccggggc
     7741 ggctccgcgc ccggctgcgc atcttccatc gggaacgtct gcgcgcccga gggcggcaat
     7801 tcgttttcgg tggccgccgg gacagcgcca tccggcgtgc tggtggcggc gctgtgcggt
     7861 gcaatggccg gctgcgcgcc ggaggtgctc gcgagaatgg cgcgctggaa acgtttcgcc
     7921 gcatcggcgt cggtggtgat gtggccggcg atgatgtccg tggccggcgc agtcttgccg
     7981 tagagcttgt tgttggactt ggtatcgatg gcgatcacgc tgccgtcgat ggagacgccg
     8041 gcaaacaacc cgcgattgcg cgagtatgaa tacacctcgg cggtgaagtt ctgatcggtg
     8101 gcggccgaag ccgcccgtcc caccgggccg gctgcgacgg aagcatcccc acccagcgtg
     8161 agcttgccgt cggtgatgcc ttcgatgctc ttgcgcgtgg tgaacaccag cacgatgtcg
     8221 gtggattgca cgccgatctg ccaacccacg ttgcccccgg taatagagac catgatgggg
     8281 ttgctgaatc ttccgttgac gtcgcgaacc accagcgcgc cgctgccgcg ccggccgcca
     8341 acgccgactg cgatcttgac gacattgggg atgacggcga tgccgtaggc gcgcgccatg
     8401 aggcggtcgg gtatgagtgt gtcgcgctgc gcgcgcaact cttcgagtac gcccgaggcc
     8461 tcgatgagcc gggcttcttc acgggcgctc gcgaaggtgg gctgaacgac gccggcgacg
     8521 ccggcggcgg caaacaacaa tgtgaccagg agctttttca tgcgcgattc ctcgctgaca
     8581 tccagatggg ccgggagctt aacgcatgcg gcataatcgc caccatgcac aggccaacat
     8641 gcactttcga ccgcgctcgc gctatcgggt tccgcctctt gttcgtggcg atttttctgc
     8701 cggctgcagc gcatgcggag
//
LOCUS       DASQJS010000158         6196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4481344, whole genome shotgun sequence.
ACCESSION   DASQJS010000158 DASQJS010000000
VERSION     DASQJS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4481344"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VEW08_06640"
     CDS             <1..296
                     /locus_tag="VEW08_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40446.1"
                     /translation="AARNVTATFNTTGTGTTCANPVTFTNNTGNFNTTGAACYRTANT
                     VNGWGCSNFDGRTVTVGGQARTCGQLPVIRSSDGYYYFSVTAGTYPWASLYTW"
     gene            complement(368..817)
                     /locus_tag="VEW08_06645"
     CDS             complement(368..817)
                     /locus_tag="VEW08_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40447.1"
                     /translation="MTDEEDLYGEVKTDVRALAQPLFELSEKALRERGSFLPHAAALT
                     EGKIRLVGAMCNTAGGVANSTHIKPLLRQGLRSMAHERPVTAIGVAETVTTTPDGQSQ
                     QAIKVLLEHHRGLTMALYLPFAQDTSGDIKFGSTLSMFVPPEINPWD"
     gene            910..1731
                     /locus_tag="VEW08_06650"
     CDS             910..1731
                     /locus_tag="VEW08_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ40448.1"
                     /translation="MNTTATPSRRMALITGASGGIGAGLAREFARRGFGVALVARRVE
                     QLETLAEDLRNAGGQASAHRGDVTVDGDIARVVAELAAMGRVPDIVIANAGFGVVGNA
                     QALSIDDYQRQFETNVFGVLRTLHETYDSLRARAGRFVIMGSVAGYLSAAGSSPYTMS
                     KFAVRALAEALHGELRPAGVSCTLVSPGFVDSDIRRVDNRGGLHADARDPIPAWLRMR
                     TDEAARVMVSGILRGKKEVIVTMHARVIVFLARHMPRLTRWILLRANRGSRPEPG"
     gene            complement(1728..2090)
                     /locus_tag="VEW08_06655"
     CDS             complement(1728..2090)
                     /locus_tag="VEW08_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40449.1"
                     /translation="MPALMILVAGPYRSGTNDDPALIQKNVDAMTDASLALFRAGHLP
                     VMGEWFALPLIEHAGSSGIGDAVFNEIFHPVSRRLVSKCDGVLRIGGPSAGADEMVAI
                     ARAHGKAVYRSMGDIPAA"
     gene            complement(2100..2864)
                     /locus_tag="VEW08_06660"
     CDS             complement(2100..2864)
                     /locus_tag="VEW08_06660"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HYJ40450.1"
                     /translation="MLLKRFGKRRVMLVALAGFFCVTGLLLATERGSVIVNFVRIKLG
                     GGFTTEERLEMHGADVESRLQPDFAAANLPYPPWEIAFIAIKQKRELQLYARPSPDTV
                     WTHVRTYPVLAQSGHLGPKLREGDGQVPEGIYRAEFLNPNSLYHLSIRLNYPNAVDRA
                     AARADGRTNLGSDIMIHGSKYSIGCIAIGNEGAEDLFILAAVVGREHVTVLITPVDFR
                     VPGTQTPANDLPWVQSLYRTLARELGNFPLASASTR"
     gene            complement(2891..3511)
                     /locus_tag="VEW08_06665"
     CDS             complement(2891..3511)
                     /locus_tag="VEW08_06665"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYJ40451.1"
                     /translation="MKVERKADQAVSETSCKSATGKTLADWFKALDKQGGVALGRRAL
                     GQWLMTEQKVEPWWSATIVGEYEISRGDFAKDGKPKGYSICPTKSIKASANDCFAAFA
                     TATALDQWFGPKHDIDLREGGHWRNADGNRATIKKVNAGKNIRLVAGDEGLTLPTPVE
                     IKFDGKGDKCTVMVSIERLQTRAEADGYRRAWGEALDRLKAKLEAG"
     gene            complement(3508..3939)
                     /locus_tag="VEW08_06670"
     CDS             complement(3508..3939)
                     /locus_tag="VEW08_06670"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HYJ40452.1"
                     /translation="MPEINWIAVVVAAVACFLLGGLWYSPMLFGKAWQRETGLTDEQL
                     KNGNMVKIFGLSLVLSLLAAWNFANFLGPRPSIAFGAGVGFSAGLLWVASSFGINYLF
                     ERKSLKLFLINGGYHTFQFTIIGLALALLPCTECAQLPCPQ"
     gene            complement(4030..4992)
                     /locus_tag="VEW08_06675"
     CDS             complement(4030..4992)
                     /locus_tag="VEW08_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40453.1"
                     /translation="MLLLAAVESSADEQPVECSARNAERATVAQIAANPDAYEGRCVA
                     VDATRRGSSLFESVDGVYLQPTDSQDPATSGLTIGLDNISRHDHESFRSVSIVGRVQD
                     CETIRNCLYSSAGENEVVMITGYCHYFDGPYLWVEKMRSRRGQPFLRRMGSGARSDYG
                     DLVPAPDAWPYRSRVAKLASEFLQALQTNDRNKLADIHFRNVGLEWEDDEAAMLRFLL
                     KDRRSPFGTIRTATTPPQQIILVERSRLEEPQFVEDYAATVCFCREKDCTGRWPIATF
                     DADNVPTRPYACTRVEPYFSRKEWVPHFTTHIGKGGLVEPDSPP"
     gene            complement(5035..5901)
                     /locus_tag="VEW08_06680"
     CDS             complement(5035..5901)
                     /locus_tag="VEW08_06680"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYJ40454.1"
                     /translation="MNEGRIQTFTHQSPIGHWSVSVWKTDPRLSEIVASMWFGEGKTA
                     YQRDRILPSGQSQLLINLGPPQYRVEPGPPEKRVPFVDVWYSGLHQGPIDTEAPHGNA
                     LLGVAFSAHGTFPWLGEHMEDLSDRIIALADALGDGALALRERLLNTATLEARFKCVE
                     RWLLARLKPRTIVHPAVRWAVDRIAATGGRIPIEALATQTGFTRKHLGNLFQQQVGVS
                     PKSLARIHRFRGALALLNRGDGQVPWAELAEQCGYYDQSHLINEFRRFTGFSPVELVR
                     RDRPDSGSVVLR"
     gene            complement(5901..>6196)
                     /locus_tag="VEW08_06685"
     CDS             complement(5901..>6196)
                     /locus_tag="VEW08_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40455.1"
                     /translation="MTPGGDLYPTLIRKNPIKPIRIYLQDGENDLSNEHGSWFLANQQ
                     MLSAFEYANAKADKDGTLGTRYEVKHNWGDGAHSDAHGGALLPDILTWIWNND"
BASE COUNT         1099 a         1913 c         2010 g         1174 t
ORIGIN      
        1 cggcggcgcg caacgtgacc gccacgttca acaccaccgg aaccggcacg acctgcgcca
       61 acccggtcac gttcacgaac aacacgggca acttcaatac caccggcgcg gcctgttatc
      121 ggaccgccaa tacggtgaac ggctggggat gctcgaactt cgatggacgg accgtgacgg
      181 tgggcggcca ggcgcgcact tgcggccagc tgcccgtaat tcgctcatcc gacggttact
      241 actacttctc ggtgaccgcc ggaacgtatc cgtgggcgag cttgtatacc tggtagcacg
      301 gcagggaacg ctctaactac cagagtgtgg cgcgagctgc cggggatgga tccccggcag
      361 ctcagcctta atcccagggg ttgatttcgg gcggcacgaa catcgacagg gtcgagccga
      421 acttgatgtc gcccgaggtg tcctgcgcga agggcaggta gagggccatc gtcagccccc
      481 ggtggtgttc cagcaggact ttgatcgcct gctgcgactg cccgtcggga gtggtggtga
      541 cagtttccgc cacgccgatc gccgtgaccg ggcgctcgtg cgccatcgac cggaggccct
      601 ggcgaagcaa cggcttgata tgggtggagt tcgcgacgcc accggccgtg ttgcacatgg
      661 cgccgacgag tctgatcttg ccttcggtga gcgcggcggc atgcggaagg aaacttccac
      721 gctcgcgcag cgccttttcc gacaattcga acaacggttg tgcgagcgcc cgcacgtcag
      781 ttttgacttc gccgtagaga tcttcttcgt cggtcaagcg aggcctccct ggctcttgtt
      841 gttgtggccg gcacttcacc acgggtgcac taacccgtca acggcggggc gtgttagaac
      901 ctgcgccgca tgaataccac agcaacaccc tcgcggcgga tggcgctcat tactggtgct
      961 tcgggcggga taggcgccgg cctggcgcgc gaattcgccc gccgcggctt cggcgtcgcg
     1021 ctggtggcgc gtcgcgtcga acagctcgag acgctcgcgg aggatttgcg caacgcgggc
     1081 gggcaggcaa gcgcccatcg cggtgacgtt acggtggacg gcgacatcgc ccgggtggtg
     1141 gcggaactcg cagcgatggg gcgggtgccg gatattgtca ttgcgaatgc cggtttcggc
     1201 gtggtcggaa acgcgcaggc cctctcgatc gatgactacc agcgtcagtt cgaaaccaac
     1261 gtgttcgggg tgctgcgaac gctgcatgag acatacgaca gcctgcgtgc acgtgccggc
     1321 cgctttgtga tcatgggcag cgtcgcaggc tacctgtccg cggcgggcag ttcgccctac
     1381 accatgagca agttcgcggt acgcgcgctc gccgaggcgc tgcatggcga actgcgcccg
     1441 gcgggcgtca gttgcacgct ggtgtcgccc ggattcgtgg actcggatat ccgccgggtc
     1501 gacaaccggg gtggcctcca cgccgacgca agggatccga tccccgcgtg gctgcgcatg
     1561 aggaccgacg aggcggcgcg cgtcatggtc agcggcatcc tgcgcggcaa gaaggaagtg
     1621 atcgtcacca tgcacgccag ggtcatcgtg ttcctggcgc gacatatgcc gcgactcacg
     1681 cgttggatcc tgctgcgcgc caatcggggc tcgcgcccgg agcctggtta ggcggcggga
     1741 atgtcgccca tcgatcgata cacggccttg ccgtgcgcgc gcgcgatggc aaccatttca
     1801 tccgcgccgg ccgatggccc gccgatgcgc agcacgccgt cgcatttact taccaaccgc
     1861 cgcgaaaccg gatggaatat ttcgttgaac accgcatcgc cgatgccgct cgagccggcg
     1921 tgttcgatga ggggcagggc gaaccactcg cccatcaccg gcaaatgccc cgcgcgaaac
     1981 aacgcgagcg aggcatcggt catggcatcc acgtttttct ggatgagcgc ggggtcgtca
     2041 ttggtgccgg agcggtacgg tccggcgacg aggatcatga gtgcgggcat ggacgtacct
     2101 tatcgcgtgg acgctgaagc cagtggaaaa ttaccgagct cgcgtgccag cgttctgtag
     2161 agagactgaa cccagggcag gtcgttggcg ggtgtctgcg taccggggac tcgaaaatcc
     2221 acgggcgtaa tcagcaccgt cacatgctct cgccccacga cggccgcgag tatgaacagg
     2281 tcttccgcgc cttcattgcc gattgcaatg cagccgatcg aatatttcga accgtggatc
     2341 atgatgtccg agccgagatt ggtgcggccg tcggcacgcg ccgctgcccg gtcgactgcg
     2401 ttcggatagt tgagccggat cgagaggtga tacaggctgt tcggattcag aaactcggcg
     2461 cggtagatgc cttcgggtac ttgcccgtca ccctcgcgca gtttgggccc gaggtgtccg
     2521 ctttgcgcca gcacgggata ggtgcgcacg tgcgtccaca cggtgtcagg ggacggccgc
     2581 gcatacagct ggagttcgcg cttctgcttg atcgcaatga atgcgatttc ccagggcgga
     2641 tacggaaggt tggccgccgc gaagtccggt tgcagccggc tctcgacgtc agcgccatgc
     2701 atttcgagtc gctcttcggt agtgaacccg ccgccaagct tgatacgcac gaaattgacg
     2761 atgaccgaac cgcgttcggt cgcgagaagc aggcccgtga cacagaaaaa accggcgagg
     2821 gcgacgagca tgacccgccg cttgccgaag cgtttcagca gcatggcgcg caacctaaca
     2881 cgcgcccgcg tcaaccagct tcgagttttg cctttagtcg gtcgagcgct tcgccccagg
     2941 cccggcgata gccgtcggct tcggctcgtg tctgcagtcg ctcgatgctg accatgacgg
     3001 tgcacttgtc gcctttgcca tcgaatttta tttcgactgg cgtgggcaac gtgagacctt
     3061 cgtcgccggc aacgagccgg atgttcttgc cggcgttcac ctttttgatg gtggcgcggt
     3121 tgccatccgc gttacgccag tggccgccct cgcgcaggtc gatgtcgtgt ttcggtccga
     3181 accattggtc gagcgccgtg gcggtggcga aagcggcgaa acagtcgttc gcgctggcct
     3241 tgatgctctt ggtcgggcag atggagtacc ccttcggctt gccatccttg gcgaagtccc
     3301 cgcgtgagat ctcgtattca ccgacgatgg tcgcgctcca ccacggctcg accttctgtt
     3361 cggtcatgag ccattggccg agagcgcggc gtccgagggc gacgccgcct tgtttgtcga
     3421 gcgccttgaa ccagtcggcc agcgtcttgc cggtggcact cttgcaggag gtttcgctga
     3481 ccgcctggtc ggctttgcgc tcgactttca ttgggggcag ggcaactgcg cgcattccgt
     3541 gcagggcaac agcgccaggg ccaggccgat gatggtgaac tggaacgtgt ggtaaccacc
     3601 gttgatcagg aacagcttca ggctcttgcg ttcgaataga tagttgatgc caaaactgct
     3661 cgcaacccag agcaggccgg ccgaaaagcc gacgccggcg ccgaacgcga tcgacggacg
     3721 cggtccgaga aagttcgcga agttccatgc ggcgagcaac gacagaacga gcgagaggcc
     3781 gaatatcttc accatgttgc cgttcttgag ctgttcgtcg gtgagcccgg tctcacgctg
     3841 ccaggctttg ccgaacagca tcggcgaata ccacaggccg cctagcagga aacacgcaac
     3901 agcagccacg acgaccgcga tccagttgat ttcaggcatg gtgccctccc ttgtttgttg
     3961 tgacgggatt ttgcgcgccg gtgcgcgccg cgtcttggaa aaaagggaag tcaggccagt
     4021 cggacgggcc tacggcggtg aatcaggctc cacgaggcca ccctttccga tgtgcgtcgt
     4081 gaagtggggc acccactcct tcctggaaaa atagggctcg acccgggtgc acgcatacgg
     4141 cctggtggga acgttgtcag cgtcaaacgt ggctatcggc cagcgaccgg tgcagtcctt
     4201 ctcgcggcag aaacagacgg tcgctgcata gtcttcgacg aactgaggct cttccagccg
     4261 cgatctttcc accaggatga tctgttgtgg gggtgtggtc gcggtccgga tcgtgccgaa
     4321 aggcgatcgg cgatccttca acaggaatcg aagcattgcg gcttcatcgt cttcccattc
     4381 cagtcccacg ttgcggaaat ggatgtcggc cagcttgttg cgatcattgg tttgcagcgc
     4441 ctgcaggaac tcgctcgcaa gttttgcgac tctcgagcgg taaggccagg catcgggcgc
     4501 cggaacaaga tcgccgtagt cgctgcgagc gccgctgccc atgcgcctca ggaaaggctg
     4561 gccgcgccgg gaccgcatct tttccaccca caagtaaggt ccgtcgaagt aatggcagta
     4621 gccggtgatc atcacgactt cgttttcgcc ggcggatgaa tacaggcagt tgcgaattgt
     4681 ttcgcaatcc tgcacacgcc ccacgattga aacgctccga aaactttcgt ggtcgtgacg
     4741 cgaaatgttg tcgaggccga tcgtcagccc ggaagtcgcc ggatcctggg aatcggtcgg
     4801 ctggaggtag acaccgtcga cgctttcgaa cagcgagctt ccccgcctgg ttgcgtcaac
     4861 ggccacacag cgcccctcgt aggcgtccgg attcgcggcg atctgcgcca ccgtcgcccg
     4921 ctccgcattg cgtgcggaac actccacggg ctgctcatcg gctgaagatt caacggcggc
     4981 caggagaagc acggccgcaa tcgcccgcaa caacctggtt cgtgcctgca cgtcctacct
     5041 cagcaccacg ctgccggaat cgggccgatc gcgacggacg agctcgacgg gcgaaaagcc
     5101 ggtgaagcgg cggaactcgt tgatgaggtg cgactggtcg taatacccgc attgctcagc
     5161 caattcggcc cagggcactt gcccgtcacc tcggttgagc aaagcgaggg cgccgcggaa
     5221 tcgatgaatg cgcgcgagcg acttggggct cactcccacc tgttgctgga agaggttgcc
     5281 gaggtgtttg cgcgtgaaac ccgtctgggt cgccagcgct tcgatgggga ttcgccctcc
     5341 cgtggccgca atgcgatcca cggcccagcg caccgccgga tgcacgatgg tgcgcggttt
     5401 caggcgcgcc agcagccagc gttcgacgca cttgaaccgc gcttcgagag tcgccgtatt
     5461 cagcaggcgc tcgcgcaggg ccagtgcacc gtcgcccagc gcatccgcga gcgcgatgat
     5521 gcggtcgctc agatcttcca tgtgttcgcc cagccatgga aacgtgccat gcgccgagaa
     5581 tgcaacacct aacaatgcat tgccgtgcgg tgcttcggtg tcgatggggc cctggtgcag
     5641 gcccgaatac cagacatcga caaagggcac acgcttctcg ggcggaccgg gctcgacgcg
     5701 gtattgcggc ggccccaggt tgatcagcag ctggctctgg cccgacggca agatgcggtc
     5761 gcgctggtac gcggtcttgc cctcaccgaa ccacatgctg gcgacaatct cgctgaggcg
     5821 ggggtcggtc ttccacacgc tcacgctcca gtgcccgatg ggcgactggt gggtgaaagt
     5881 ctggatgcgg ccttcgttca tcagtcgttg ttccagatcc aggtgaggat gtcgggcagc
     5941 aacgcaccgc catgcgcgtc cgaatgcgcg ccgtcgcccc agttgtgctt cacctcgtag
     6001 cgcgtgccga gcgttccgtc cttgtcggcc ttggcgttcg catattcgaa cgccgaaagc
     6061 atctgctggt tggccaggaa ccagctgcca tgctcgttcg acaggtcgtt ctcgccgtcc
     6121 tgtagataga tgcggatggg cttgatggga ttcttgcgga tcagcgtggg atacaggtcg
     6181 ccgcccggcg tcatgg
//
LOCUS       DASQJS010000159        14849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3540003, whole genome shotgun sequence.
ACCESSION   DASQJS010000159 DASQJS010000000
VERSION     DASQJS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14849
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3540003"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VEW08_06690"
     CDS             complement(<1..502)
                     /locus_tag="VEW08_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004654475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ40456.1"
                     /translation="MASAGVFDVIIVGGGHAGTEAALAAARMGARTMLLTQSIETLGQ
                     MSCNPAIGGIGKGHLVKEIDALGGVMARGADAAAIQSRTLNASKGPAVRATRVQADRV
                     LYKAFIRRTLENQPNLTLFQQECADLIVENGAVCGVVTVTGIEFRARTVVLTVGTFLG
                     GRIHVGL"
     gene            626..949
                     /locus_tag="VEW08_06695"
     CDS             626..949
                     /locus_tag="VEW08_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40457.1"
                     /translation="MKKSNVLAGIGLVLGMAMVGSANADTPRYDARQHNQRQRIVNGV
                     QNGELTMRETRRLAGGQVHLNRVERRAEADGVVTRRERAHMQHEENQQSRRIYRQKHD
                     AQDRS"
     gene            1085..2557
                     /locus_tag="VEW08_06700"
     CDS             1085..2557
                     /locus_tag="VEW08_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ40458.1"
                     /translation="MMRLCVLAALIGLAGPAAATSVPSQLALKPCRLEHPARMLALTA
                     ECGTLTVAENPGQPDGRKIDLFVARVPAISLNKKPDPLFLIAGGPGTSAVDLYTSSAG
                     SFGRVRRDRDIILVDQRGTGRSHRLDCDYGDQNIFERIDEIEVGEANIKCRDELSRNA
                     DLRMYTTSVAVRDLEHARQALGYERINLYGNSYGTRVAQHYARRYPKSTRTVILDGVV
                     NPEVVLGPAIAIDAERALDRILERCTANADCARAFADPAADYRDLRARLTAKPERTMV
                     SDVATGRPIHFDFTSRHLSAVLRFASYNDDQAALLPLSLHLATHEGNFTPLASQFRVF
                     ANSLESSFAYGMHNSVTCSEDAPLIDASKLDLTVLKATHMGAEQVQQLIEACKDWPKG
                     VMDSDLHASFKSAAAALLLSGADDPVTPPQSATIAQRAFADSKHVIIAGHGHGQLGAP
                     CVDRIIEDFVTAGTARGLDTSCTSKLKPMPFFITLAGPAP"
     gene            2562..3296
                     /locus_tag="VEW08_06705"
     CDS             2562..3296
                     /locus_tag="VEW08_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ40459.1"
                     /translation="MLEARGLFKSFGKVEAVRGVSFAARDGQITGLLGPNGAGKSTTL
                     RMLYTVLKADQGDAFIDGTSVTADPLAARRSIGVLSHGAGVYNNLTARENITYFGELH
                     GMERADRDARAAELIEMLEMQPFADRLAKGFSQGQKLKTALARALVHRPRNLLLDEPT
                     NGLDVMAVRNLRKLLGQLRDAGHCVLFSSHVMQEVEHLCDEVVVINGGQVVAAGTLAD
                     IRARVANSHLEDAFVSLMDGDGGESK"
     gene            3293..4477
                     /locus_tag="VEW08_06710"
     CDS             3293..4477
                     /locus_tag="VEW08_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ40460.1"
                     /translation="MSLRGIWTVFRKEFRENLRDRRTLLSALVFGPVISPILVAVLVQ
                     FLISRTETQADQDIPLAVIHAEHAPNLLNYLEARGINIVKVKFDEAGARLAVAKQTHN
                     IVMEIPPDFGQRLQAGEPAPVVLYSDSSRGFERQGVSRVRALVANYGIEIAQLRFLAR
                     GIDPISVLPISVQEIDVSTPSGRSVFVLNMMTYLVLLSMLFGGLYLAIDATAGERERG
                     SLEVLLTSPVPREHLIYGKILAAAAYMFISLVLTVTMFALAMSFVGLERLGASANLGP
                     APAALLVGCCASLILFGSAYLTIISAFAKTYREAQTYLQLVVTIPTMPLIFAGLLGLQ
                     TKTSLMFVPFLSQHLLMTTVVRAEQIEPLHILVSIGSTLVYGAVLTYIAGRLYRREAL
                     LG"
     gene            complement(4469..4897)
                     /locus_tag="VEW08_06715"
     CDS             complement(4469..4897)
                     /locus_tag="VEW08_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40461.1"
                     /translation="MWFGNRCYPPRDVQLALESLFGESVGKIKVVQYSIFVRFHWRAI
                     ATTRPNRIYLRGSGKEFFADGPLVLHEYFHVIRQWGTGELTIPRYLIECFRRGYWDNR
                     FEIEAREYTDTEIFRYRALLAQHRTAELPPGVHPIRKKSA"
     gene            complement(4899..6251)
                     /gene="mnmE"
                     /locus_tag="VEW08_06720"
     CDS             complement(4899..6251)
                     /gene="mnmE"
                     /locus_tag="VEW08_06720"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019580343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HYJ40462.1"
                     /translation="MSHPDTIVAAASPPGRGGVGVVRVSGPKTPEIAATLVGELPKPR
                     HATLSRFVDRAGNPIDIGIALFFPGPLSYTGEHVLELQGHGGPVIVEQLIERCLELGA
                     RRARPGEFTERAFHNDKIDLAQAEAVADLIDAGSREAARAAMRSLQGEFSGMVQGLTD
                     SLIDLRTYVEAAIDFPEDEVDFLGDLELQERLATVRGHFDAVSAAASQGRLLRDGMTV
                     VLAGRPNSGKSSLLNRLAGYEAAIVTAIPGTTRDVLRERIDIDGLPLHVLDTAGLRDS
                     DDVVEREGVKRATAEMSRADRVLFVIDTSTDPDGRAFDDERARLPANVPVTLLFNKID
                     LGEGLPIADTLAGPPRIHVSAVTGLGLDVLRAHLKDSVSFQVAGSGTISARRRHLEAL
                     ARARECVETAARTLTQRRAGELVAEELRQGQQALEEITGVFTADDLLGRIFGSFCIGK
                     "
     gene            complement(6381..8096)
                     /gene="yidC"
                     /locus_tag="VEW08_06725"
     CDS             complement(6381..8096)
                     /gene="yidC"
                     /locus_tag="VEW08_06725"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HYJ40463.1"
                     /translation="MTNNIRVFLWLAVALALWLNYSQWQIDYGAKPVTTSTGAVETDS
                     SGAPKPSDIADAVPQVVQPATDAPVASDAVPAVATQQAAAGPDIESAKTRKIRVTTDV
                     LVLDISLTGGTLVRAELPGYPLIKGEAAPVVLFNTDAPTTNYVLRTGLASSDDPNGPS
                     HLATFTAPADSFTLAAGQDELRVPLTWTDGKGVTVTKTFVFKRSMYAIDLEYQIVNGT
                     QAPWHAVSYARIVRSDPPVERSMFKVESYATRGPAFGEGDKYKKLNIEKTDDATLSIK
                     VTNGWIAGMQHHFVSAVVPDHETPHTFTMGVKGREYVLTSLGEVQTVAPGATRTIKET
                     LFVGPKLQKQLETLHPELSRVADYGVLTLISRPLFWLLDHAHSIVKNWGLAIILVTFM
                     LKLLFYPLSEKAGRSMARMRALAPRMKILQETYKDDRTKLGQATMELYKQEKVNPLAG
                     CLPMLIQMPVFLAFYWVLLESVEMRQAPFFGWINDLSARDPYFILPVLNGLAMWGQSK
                     LNPPPPDPVQARIFQLMPIVFSVMFALFPSGLVLYWVTNTGLSILQQWNINRKITAED
                     AARKR"
     gene            complement(8086..8322)
                     /gene="yidD"
                     /locus_tag="VEW08_06730"
     CDS             complement(8086..8322)
                     /gene="yidD"
                     /locus_tag="VEW08_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HYJ40464.1"
                     /translation="MRFVLKYLIRGYQLAISPLLGPRCRFYPTCSQYAIEAIETHGAL
                     RGVWLTIKRISRCHPWHEGGFDPVPACLNHSHDQ"
     gene            complement(8307..8678)
                     /gene="rnpA"
                     /locus_tag="VEW08_06735"
     CDS             complement(8307..8678)
                     /gene="rnpA"
                     /locus_tag="VEW08_06735"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HYJ40465.1"
                     /translation="MQPTPRRLGFGAELRLRSKLQFDAIYAGGRRIDDRFFGLRVKPN
                     GLTHARVGLAVAVKTAGNAVARNRLRRLVRESFRLAQHGLPAVDIVVAAKFPAREAPA
                     ATLRASLATLWQRVATTCASS"
     gene            complement(8710..8844)
                     /gene="rpmH"
                     /locus_tag="VEW08_06740"
     CDS             complement(8710..8844)
                     /gene="rpmH"
                     /locus_tag="VEW08_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HYJ40466.1"
                     /translation="MKRTYQPSKIKRKRATGFRARMKTTGGKKVLARRRSKGRKKIVP
                     "
     gene            9040..10362
                     /gene="dnaA"
                     /locus_tag="VEW08_06745"
     CDS             9040..10362
                     /gene="dnaA"
                     /locus_tag="VEW08_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HYJ40467.1"
                     /translation="MRCMRALEDELPGDHFNMWVRPLQAVESSGALKLLAPNRFAVDW
                     VNAHLLSRLGEIVRRFVEGDAPIVTVEVGSRAPELVQPVEAAANGAARPKRSEGIVVG
                     VRLNPDFTFNGFVEGKSNQLAKAAAVQVAENPGRAYNPLFIYGGVGLGKTHLMQAVGH
                     TIKARDPEARVAYIHSERFVSDMVIALQHNKMNEFKTAYRSLDALLIDDIQFFANKDR
                     SQEEFFHTFNALLEGQQQVIVTADRYPKEVSGLEERLKSRFGWGLTVAIEPPELETCV
                     AILIGKAQAANVMLPEEVAFFIAKRIRSNVRELEGALKRVIANSRFTGKAITLDFTKE
                     ALKDLLTLQAKLITIENIQKTVADYYKVRVADLLSKRRSRSIARPRQVAMALAKELTT
                     HSLPEIGDAFGGRDHTTVLHACGRIRELRVAEQRVGEDYQNLLRTLTG"
     gene            10567..11664
                     /gene="dnaN"
                     /locus_tag="VEW08_06750"
     CDS             10567..11664
                     /gene="dnaN"
                     /locus_tag="VEW08_06750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HYJ40468.1"
                     /translation="MKLSATREQILAPLQSVIGVVERRQTMPVLSNVLLAARNNRVSV
                     TGTDLEVELVATSDATVQQPGDITVPGRKLLDIFRALPEKTAVTVATDGERVSIKAGK
                     SRFTLSTLPAAEFPIVEEINAQQTLTLSQGDFRRLIDKTHFSMAQQDVRYYLNGLLLE
                     TDGKILRAVATDGHRLALCEAELESKAKTNQQVILPRKGVLELQRILGGDGNVDLAIG
                     TSHVRAQIGDIRFTSKLIDGRFPEYGRVIPANPTKQVEADREGLRQALQRTAILSNEK
                     YRGVRINLKSDLVTIQAHNPEQEEAEDQVEVTYTGDEVEIGFNVTYLLDALAAVDSDK
                     VILGLTDGNSSCLVRAAGTTNCKYVVMPMRL"
     gene            11689..12744
                     /gene="recF"
                     /locus_tag="VEW08_06755"
     CDS             11689..12744
                     /gene="recF"
                     /locus_tag="VEW08_06755"
                     /inference="COORDINATES: protein motif:HMM:NF000819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HYJ40469.1"
                     /translation="MPLAELRVENLRCIESAVLEFSPELNLIAGENGAGKTSILEAIF
                     LLGRGRSFRTRSSERLIRYHQPKLTVFGRTDDSPTRQAGIEVAADGGTRARIDGEAAT
                     SLLELSGVLPVQAIDPEIHKLVDQGPERRRRWLDWLVFHVEPSFGIQWARYNRALKQR
                     NASLRVGGESSAWDAELIRNGIAITESRQRALSRLVPCLEQTFERFGGLDVSVGFTTG
                     WLAGTSFEEALRTHAERDRARGTTTAGPHRADVTLRRNQRIARETLSRGQQKLTAVAM
                     IVSQLRLLQEELNLRAVLLLDDPAAELDDKNLQRLFEELSALRCQMIATSLTQETALF
                     QAPKATFHVEQGRVIRV"
     gene            12771..>14849
                     /gene="gyrB"
                     /locus_tag="VEW08_06760"
     CDS             12771..>14849
                     /gene="gyrB"
                     /locus_tag="VEW08_06760"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HYJ40470.1"
                     /translation="MANDDVYDSSKIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFE
                     VVDNAIDEALAGHCDRIVVTIHADESVTVSDNGRGIPTDIHPEEGRSAAEVIMTVLHA
                     GGKFDDSSYKVSGGLHGVGVSVVNALSDHLWLTINRDGKAHKMEFRLGDPVAPLAIAG
                     DTTEKGTVVRFKASPTIFTNITFDYEILAKRLRELSFLNSGVRIELIDEREAKNDVFQ
                     HEGGLQEFVKHLNRSRTGIHPSVFWFRTQEGPIGVEVAMQWNDSYTESMFCYTNNIPQ
                     KDGGTHLSGFRAALTRTLNDYIEKEMAAKKEKVPTTGDDSREGLTAILSVKLPDPKFS
                     SQTKEKLVSSEVKGVVESAVTEKLSEFLLEHPNEAKAIVGKIIDAARAREAARKAREM
                     TRRKGALDIAGLPGKLADCQEKDPALCEIFIVEGDSAGGSAKQGRDRRTQAILPLKGK
                     ILNVEKARFDKMLQSAEVGTLITALGCGIGRDEFDINKLRYHRIIIMTDADVDGSHIR
                     TLLLTFFYRQIPLLIERGHIYIAQPPLFKVKRGKQEVYVKDELELNTMLLASALDDAA
                     LHVNSAAPALSGPGLQGLARQYMEVQAIIKRWARRYDERLLDQLIYMPAVSAAEFERA
                     DWMRDWTRDLALHLNGLSDSPRSYRVELRQVDGHPRRVLVSKTEHGSITEKALPREFF
                     ESAEYRRIVEL"
BASE COUNT         2878 a         4624 c         4634 g         2713 t
ORIGIN      
        1 cgagaccgac gtgaatacgg ccgccgagga atgtgccgac ggtgagcacc accgtgcgcg
       61 cgcggaattc aatgccggtc acggtgacca cgccacacac cgcgccgttc tcgacgatga
      121 gatcggcgca ctcctgctgg aacaacgtga gattgggctg gttctcgagc gtgcggcgaa
      181 tgaaggcctt gtacagcacg cgatccgcct gcacgcgcgt cgcgcgcaca gccggaccct
      241 tgctggcgtt caacgtgcgt gactggatag ccgccgcatc ggcgccacgc gccatgacgc
      301 cgcccagcgc gtcgatctct ttgaccaggt ggcctttgcc gatgcccccg atggcggggt
      361 tgcagctcat ctggcccagc gtctcgatgc tctgcgtgag cagcatcgtg cgcgcgccca
      421 tgcgcgctgc cgcgagcgcg gcttctgtgc ccgcatgccc gccgcccacg atgattacgt
      481 caaaaacgcc tgcagaagcc acggaatcgc cgccagatca aggagttaag gcggcggatg
      541 gtatatcggc caaggtgtca acgccaccgg gggcacggcg ttttctatct caacagagcc
      601 gaattccggc ttcaggagag aagacatgaa gaagtcgaac gttttggccg gtatcggtct
      661 ggtccttggg atggcgatgg tgggttccgc gaatgccgat accccgcgct atgacgcgcg
      721 ccagcacaac cagcgccagc gcatcgtgaa cggtgtgcag aacggcgagc tgaccatgcg
      781 cgagacgcgc cgccttgccg gtggccaggt gcatctgaat cgggtcgagc gccgcgccga
      841 ggccgatgga gtggtgacgc gccgtgagcg agcccacatg cagcacgaag agaaccagca
      901 gtcgcgccgc atctatcgcc agaagcacga cgcccaggac cgcagctgaa cgtagaaccg
      961 taagaatgaa gggcacgtat cgcagggtac gtgccctttt ttcacattca cggacccggc
     1021 gcagccggtt tttaatcccg ggcgcagccc gtgggaacgc cgtggcatgt atcctgcgcg
     1081 ccgcatgatg cgactttgcg ttctggcagc gttgataggc ttagccggcc ctgcggccgc
     1141 gaccagcgtc ccgtcgcagc tcgcgctcaa accgtgccgc ctcgagcatc cggcgcgcat
     1201 gctggcgttg accgcagagt gcggcacgct gaccgtggcg gagaatcccg ggcaacccga
     1261 cggtcgcaag atcgacctct tcgtcgcgcg cgtcccggct atcagcctca acaagaaacc
     1321 cgacccgctg tttctcatcg ccggaggccc cggcacgtcg gccgtcgacc tgtacacctc
     1381 gtccgccggt tcgttcggtc gcgtgcgccg cgatcgcgac atcattctcg tggaccaacg
     1441 cggcacgggc cgctcgcatc ggctcgattg cgactatggc gatcagaaca tcttcgagcg
     1501 catcgacgaa atcgaagttg gcgaggccaa catcaagtgc cgcgatgagc tttcgaggaa
     1561 tgcggatctg cgcatgtaca cgaccagtgt cgcggtgcgt gatctcgagc acgcgcgaca
     1621 ggctctcggg tacgagcgca tcaatctcta tggcaattcc tacggcaccc gcgtggcgca
     1681 gcactatgcg cgccgctatc cgaaatccac gcgcaccgtg attctcgacg gcgtcgtaaa
     1741 tccggaagtg gtgctggggc cggccatcgc catcgatgcg gagagagcgc tggatcgcat
     1801 cctcgagcgc tgcactgcaa acgccgactg cgccagggcg ttcgccgacc ctgccgcgga
     1861 ctatcgcgac ctgcgcgcgc ggctcacggc caagcccgaa cgaacgatgg tcagcgacgt
     1921 cgccacgggc aggccgattc atttcgactt cacctcgcgg catttgtccg ccgtactgag
     1981 attcgcgagt tacaacgacg accaggccgc actcctgccg ctgtcgttgc acctggcgac
     2041 gcacgaaggg aatttcacgc cgctcgccag ccagttccgc gtattcgcga attcgctcga
     2101 gtcgtcattc gcctacggca tgcacaacag cgtgacctgc agcgaagatg cgccgctgat
     2161 agacgcgtcg aaactcgatc tgactgttct caaggccacg cacatgggcg ccgagcaggt
     2221 gcagcagctc atcgaagcct gcaaggactg gccgaagggc gtcatggata gcgaccttca
     2281 cgcctctttc aagagtgcgg ccgccgccct tctgttgtcg ggcgcggatg acccggtgac
     2341 tccgccgcaa tccgccacga ttgcccagcg cgcattcgcc gacagcaaac acgtgataat
     2401 cgccgggcat ggccacgggc agctgggcgc tccctgcgtg gaccggatca tcgaggactt
     2461 cgtgacggcg ggtacggccc gcgggctcga cacgagttgc accagcaaac tcaagccgat
     2521 gccgtttttc atcacgctgg cgggacccgc accgtgaagc catgttagaa gcccgcggcc
     2581 tgttcaagtc tttcggcaag gtcgaagcgg tccgcggcgt gagcttcgcc gcgcgcgatg
     2641 gacagatcac cgggttgtta ggcccgaacg gcgccggcaa gtccaccacg ctgcgcatgt
     2701 tgtacacggt gctgaaggcg gaccagggcg acgcgttcat cgatggcacc agcgtcacgg
     2761 cggatccgct ggcggcgcgg cggtcgattg gcgtgctgtc gcacggggcc ggcgtctaca
     2821 acaacctcac cgcgcgcgag aacatcacct acttcggcga gctgcacgga atggaacgcg
     2881 ccgatcgtga cgcgcgcgcc gccgaactca tcgaaatgct cgagatgcag ccgttcgccg
     2941 atcggctggc gaagggattt tcgcaggggc agaagctcaa gaccgcgctg gcacgcgcgc
     3001 tggtgcaccg gccgcgcaac ctgttgctcg atgaacccac caacggcctc gatgtgatgg
     3061 cggtgcgcaa tctgcgcaag ttgctgggcc agttgcgcga cgcgggtcac tgcgtactgt
     3121 tctcgagcca cgtcatgcag gaagtcgagc atctctgtga cgaagtcgtg gtgatcaacg
     3181 gcggccaggt ggtggccgcg ggcacgctgg cggatatccg tgcgcgtgtc gccaacagtc
     3241 atctcgagga cgcgttcgtc agcctcatgg atggcgatgg cggtgaatcg aaatgagcct
     3301 gcgcggcatc tggacggtgt tccgcaagga attccgcgag aacctgcgcg accgacgcac
     3361 gctgctgtcg gcgctggtgt tcgggcccgt gatcagtccg attctcgtcg cggtgctggt
     3421 gcagttcctg atttcgcgca ccgagacgca ggctgaccag gacattccgc tggcggtgat
     3481 tcatgccgaa cacgcaccca acctcctcaa ttacctggag gcacgcggca taaatatcgt
     3541 gaaggtgaag ttcgacgagg cgggtgcgcg cctggcggtc gccaaacaga cgcacaacat
     3601 cgtgatggag atccccccgg acttcggtca gcgcctgcag gccggcgaac ccgcgccggt
     3661 ggtcttgtac tcggacagct cacgcggctt cgaacgccag ggcgtatcgc gcgtgcgcgc
     3721 gctggtggcc aactacggca tcgagattgc gcagctgcgt tttctcgcac gcggcatcga
     3781 tccgatttcg gtgctgccga tttccgtgca ggagatcgac gtctccacac ccagcggccg
     3841 ttcggtattc gtgctcaaca tgatgactta cctggtgctg ctgtcgatgt tgttcggcgg
     3901 cctgtacctg gccatcgacg ctaccgccgg tgagcgtgaa cgcggctcgc tcgaagtgct
     3961 gctcacttca cccgtaccgc gcgagcacct gatctacggc aagatcctcg cggcggcggc
     4021 atacatgttc atctcgctgg tgctcacggt gacgatgttc gcgctggcca tgagcttcgt
     4081 gggcctggag cggctggggg cgtccgcaaa cctggggccc gcacccgcgg cactgctcgt
     4141 gggctgttgc gcgtcgctga tcctgttcgg ttcggcctat ctcaccatca tctccgcctt
     4201 cgccaagacc tatcgagagg cgcagaccta tctgcaactc gtggtgacga tcccgaccat
     4261 gccgctgatc ttcgcgggac tgttgggcct gcagaccaag acgtcgctca tgttcgtgcc
     4321 gttcctgagc cagcacctgt tgatgacgac cgtcgtacgt gctgaacaga tcgaaccgct
     4381 gcacattctg gtgtcgatcg gaagcactct ggtgtacgga gcggtgctca cgtacatagc
     4441 cggccggctg taccggcgcg aggcgctgtt aggctgactt cttgcggatc gggtgaacgc
     4501 ccggcggcaa ctcggccgtg cggtgctgcg cgagcaacgc gcggtagcga aagatttccg
     4561 tatcggtgta ttcgcgcgct tcgatttcga agcgattgtc ccagtagccg cggcgaaaac
     4621 attcgatgag ataacgcggg atggtcagct cgcccgtgcc ccactggcgg atgacgtgga
     4681 agtattcgtg cagcaccagc ggaccatcgg cgaaaaactc cttgccgctg ccgcgcaggt
     4741 agatccggtt cgggcgcgtg gtggcgatgg cccgccagtg gaagcgaacg aagatgctgt
     4801 actggacgac cttgattttc ccgacggatt cgccgaacag gctctcgagt gcgagctgaa
     4861 catctctcgg gggataacaa cgattaccaa accacatgtc acttccctat gcagaaggaa
     4921 ccgaagatac ggcctaacaa gtcgtccgcg gtaaagacac cggttatttc ctcgagcgcc
     4981 tgctggccct ggcgcaattc ctcggccacc agttcgccgg cgcgccgctg ggtgagcgtg
     5041 cgggcggccg tctcgacgca ttctcgcgcc cgcgccagcg cttcgagatg ccggcgccgt
     5101 gcggaaatgg ttccactgcc cgcgacctgg aagctcacgc tgtccttgag gtgcgccctc
     5161 aagacgtcga gccccaggcc cgtgacggcc gacacgtgaa ttcgcggcgg acctgccagg
     5221 gtgtccgcga tgggcaatcc ctcacccagg tcgatcttgt tgaacagcag cgtgacgggc
     5281 acgttcgccg gcagtcgagc gcgctcgtcg tcgaaggcgc ggccatcggg atcggtggag
     5341 gtatcgatga cgaacagcac gcggtcggcg cgggacatct cggcggtcgc gcgcttcacg
     5401 ccttcccgct ccacgacatc gtccgaatcc cgcaggcctg cggtgtcgag cacgtgcagc
     5461 ggcaatccgt cgatgtcgat gcgctcgcgc agcacatcgc gcgtggtgcc cggaatggcg
     5521 gtgacgatcg ccgcttcgta tcccgccaac cggttgagca ggctggattt gccggagttc
     5581 ggccggcccg cgagcaccac ggtcatgccg tcacgcagca gccggccctg gctcgcagcc
     5641 gcggaaacgg catcgaaatg gccgcgcacg gtagctagcc gctcctgcag ctccaggtcg
     5701 ccgagaaagt cgacttcatc ctcgggaaaa tcgatggcgg cttccacgta agttcgcaag
     5761 tcgatcagtg aatcggtgag tccctggacc atgcccgaga actcaccttg cagggagcgc
     5821 atggcagcgc gtgccgcttc gcgcgagccc gcatcgatga ggtcggccac ggcttcggcc
     5881 tgggcgagat caatcttgtc gttgtgaaaa gcgcgctcgg tgaactctcc cgggcgcgcg
     5941 cgccgcgcgc cgagctcgag acagcgctcg atcagctgct ccacgatcac gggtccaccg
     6001 tggccctgca gctcgagcac atgctcgccg gtgtacgaca gcgggccggg gaaaaacagc
     6061 gcaatgccaa tgtcgatcgg attgccggct cggtcgacga agcgcgaaag agtcgcgtga
     6121 cgcggctttg gcagttcacc gacgagcgtc gcggcgattt ccggtgtctt ggggcccgac
     6181 acgcgcacga cgccgacccc gcctcgtccg ggcggtgatg ccgctgccac tatggtgtcg
     6241 gggtgactca tacatgcagc ggccctggcg ccgctggtga gcagtctacg cccgttgccg
     6301 catgccgccg ttcgctgcag cgcgtcagcc gatgagagaa ggcgcggtta tttcttccgc
     6361 gcccggcccg cgttagtccg ctatctcttg cgggccgcgt cttcggcagt gattttccgg
     6421 ttgatgttcc actgctgcag gatggacaag cccgtgttgg tgacccagta caacaccagg
     6481 ccggacggga acagcgcgaa catcacggag aacacgatgg gcatgagctg gaagattcgc
     6541 gcctgcaccg gatccggggg cggcgggttc agcttcgatt gaccccacat cgcgaggccg
     6601 ttcagcaccg gaaggatgaa gtacgggtca cgcgccgaca ggtcgttgat ccagccgaag
     6661 aacggcgcct ggcgcatttc gacgctctcg agcagcaccc agtagaacgc caggaacacc
     6721 ggcatctgga tgagcatggg caggcagccg gcgagagggt tcaccttttc ctgcttgtac
     6781 aactccatcg ttgcctggcc gagcttggtg cggtcgtcct tgtaggtttc ctgaaggatc
     6841 ttcatgcgcg gcgcgagcgc gcgcatgcgc gccatcgagc ggccagcctt ctccgacaac
     6901 ggatagaaga gcagcttgag catgaacgtc acgagaatga tcgcaaggcc ccagttcttg
     6961 acgatcgagt gcgcatgatc gagcagccag aacagcggcc gcgagatcag cgtgagtaca
     7021 ccgtagtccg cgacacggct gagttccgga tgcagggtct cgagctgctt ctgcagcttc
     7081 gggccgacga acagagtttc tttgatcgtc ctggtagctc ccggcgccac ggtctgcact
     7141 tcacccagcg acgtcaaaac gtattcacgg cccttcacgc ccatggtgaa cgtgtgcggt
     7201 gtttcgtgat cggggacgac cgcgctcacg aaatgatgct gcatgccggc gatccagccg
     7261 ttcgtgacct tgatcgacag ggtcgcgtcg tcggtctttt cgatattgag cttcttgtac
     7321 ttgtcgcctt cgccaaaggc cggtccacgc gtcgcgtagc tttcgacctt gaacatggaa
     7381 cgctcgaccg gcgggtccga tcgcacgatg cgcgcgtatg acacggcatg ccacggggcc
     7441 tgtgtgccgt tcacgatctg atactcgaga tcgatggcgt acatgcttcg cttgaagaca
     7501 aaggtcttgg tgaccgtgac gcctttgcca tcggtccagg tcagcggcac acgaagctcg
     7561 tcctggccgg cagcgagcgt gaagctgtcg gcgggagccg tgaacgtcgc caggtgcgag
     7621 ggcccgttcg gatcatccga actggcgagg ccagtgcgca gcacgtaatt ggtggtcggc
     7681 gcatcggtgt tgaacaacac cacgggcgca gcttcaccct tgatcagcgg ataaccgggc
     7741 agctcggctc ggacaagggt cccacccgtc agcgagatat cgagcaccag cacgtccgtc
     7801 gtcacgcgaa tcttgcgggt cttggcgctc tcgatgtcgg ggccggcggc cgcctgctgg
     7861 gtcgccacgg cgggcaccgc gtcgctggca accggcgcgt cggtcgctgg ttgtaccacc
     7921 tgcgggacag catccgcgat atccgacggc tttggcgcgc cggagctgtc agtttcaacg
     7981 gctccggtgg aggtcgttac cggcttcgca ccgtagtcga tctgccactg cgaatagttg
     8041 agccacaagg cgagcgcgac ggccagccat aaaaagacgc gaatgttatt ggtcatgcga
     8101 atgattgaga caggccggga ccggatcgaa tccgccttcg tgccaggggt gacaacggga
     8161 aatccgtttg atggtgagcc acacgccgcg cagggcgccg tgggtttcga tggcttcgat
     8221 cgcgtactgc gagcacgtcg ggtagaaacg gcagcgcggc ccgagcagcg ggctgatggc
     8281 gagctggtac ccgcggatca gatatttcag gacgaagcgc atgtcgttgc gacgcgttgc
     8341 caaagggtcg cgaggctagc acgcagcgtt gccgccggtg cttcacgcgc gggaaatttt
     8401 gcggccacca cgatgtccac tgccggaagc ccgtgctggg ccagtcggaa actctcgcgc
     8461 accaggcgtc gcagtcgatt tcgcgccacg gcattgccag cagttttcac cgccacggca
     8521 agtcccacgc gggcatgcgt gagcccgttg ggcttgacgc gaagtccgaa gaatcggtcg
     8581 tcgatgcgcc gcccaccggc atagatcgca tcgaactgca gcttgctgcg caggcgcagt
     8641 tctgcgccaa aacctaaacg acgtggtgtg ggctgcattg cggcccgctg tggacgagct
     8701 cccgcgtctt taagggacga tcttcttgcg gcccttggag cggcggcgtg caaggacctt
     8761 tttcccaccc gtggttttca tgcgggcgcg aaaacccgta gcgcgtttgc gcttgatctt
     8821 gctcggctga taagtgcgtt tcatggtgat cggcccgaag ccgctgatat cccggaaaaa
     8881 agggcgggaa ggttacgcag cagggcgtcc gggtgtcaat acaatccggt caggaacggc
     8941 tagacttcgc accctttttt ccggcccgag tcccaaacgg atccatctgt gactgcagcg
     9001 tctcgcgata catcacagct ggatggcggc acgctctgga tgcgctgcat gcgcgccctg
     9061 gaggacgagc tgccgggcga tcacttcaac atgtgggttc gccccctgca ggcggtcgaa
     9121 agcagcgggg ccctcaagtt gctcgctccg aaccgctttg ccgtggactg ggtgaatgcc
     9181 cacctattgt cgcgtctggg cgagatcgtg cggaggtttg tcgaggggga tgctccgatc
     9241 gtgaccgtcg aagtgggttc gcgcgccccg gagctggtgc agccggttga ggcggccgcc
     9301 aacggcgctg cgcgccccaa gcgcagcgaa ggcatcgtcg tcggggtccg gctgaatccg
     9361 gatttcacgt tcaacggttt cgtcgagggc aagagcaatc agctcgcgaa ggccgcggcg
     9421 gtgcaggtcg ccgagaatcc aggacgagcc tacaacccgc tgttcatcta tggcggtgtc
     9481 ggtctcggca agacgcatct catgcaggcc gtgggtcaca ccatcaaggc gcgcgatccg
     9541 gaagcgcggg ttgcctacat tcactccgag cgtttcgtga gcgacatggt gatcgcgctt
     9601 cagcacaaca agatgaacga gttcaagaca gcgtatcgct cgctcgacgc gctgctcatc
     9661 gacgacattc aattttttgc gaacaaggat cgctcgcagg aagaattttt ccacacgttc
     9721 aacgcgctgc tcgaagggca gcagcaggtc atcgtcacgg ccgaccgtta tccgaaagaa
     9781 gtttcgggcc tggaggaaag gcttaaatcc cgcttcggct gggggctgac ggtagccatc
     9841 gagccgccgg aactcgaaac ctgcgtcgcg attctcatcg gcaaggccca ggccgcgaac
     9901 gtgatgcttc cggaagaagt ggccttcttc atcgcgaaac gaattcgttc caacgtgcgc
     9961 gagctcgagg gcgcattgaa gcgcgtgatc gcgaattcgc gattcacggg caaggccatc
    10021 acgctggatt tcaccaagga agcgttgaaa gacctgctga cattgcaggc gaaattgatc
    10081 actatcgaaa acatccagaa gacggttgcc gactactaca aagttcgcgt ggcagatctg
    10141 ctttcaaaac gccgtagtcg ttccatcgcg cgaccgcgcc aggttgctat ggcgctcgcg
    10201 aaagaattga cgacgcatag ccttccggaa atcggtgacg ctttcggcgg gcgagatcac
    10261 accacggtgc tgcatgcctg cggtcgaatc cgggaattgc gcgtggctga acagcgagtg
    10321 ggtgaggatt atcaaaacct gttgagaacc ctgaccggat gactgtggaa tgcctgtgga
    10381 cagttttgtg gacaactttg tccacagatc acacacaggg gatcatcagg ctgtgggtaa
    10441 gttcgttagg tagggaatga ggggcaagag actgattggc agatggtttt ggattaatcc
    10501 ccatcatcca caggctctac tgctgctact actggcttta aatcattaag aacagaaggc
    10561 aatcgcatga agctctccgc gacccgcgag caaattcttg ctccgctcca aagtgtcatt
    10621 ggtgtcgtcg aacgtcggca aaccatgccg gttctctcca atgtgctgct ggctgcgcga
    10681 aataaccgcg tcagcgtgac cggtacggat ctcgaggtgg agctggtcgc gaccagcgat
    10741 gcaaccgttc agcagccagg ggacatcacg gtcccgggcc gaaaactgct ggatatcttt
    10801 cgtgcgctgc ccgagaaaac ggcggtaacg gtcgcgactg acggtgaacg tgtatcgatc
    10861 aaggcgggaa aatcgcgttt cacgctctcc actcttccgg ccgctgagtt tcccatcgtc
    10921 gaagaaatca atgcccagca aacgttgacc ctctcgcagg gagatttcag acgtttgatc
    10981 gataagacgc acttttcaat ggcacagcaa gacgttcgct actatttgaa cggacttttg
    11041 ctcgaaaccg acggaaaaat cctgcgcgcc gtggctacag acggccaccg gctggcgctg
    11101 tgcgaggcgg agctcgaatc gaaggcgaag acgaaccagc aggtcatcct gccgcgcaag
    11161 ggtgtgctcg agcttcagcg cattctgggc ggcgatggca acgtagactt ggcgatcggt
    11221 accagccacg ttcgtgccca gatcggcgac attcgcttta cttcgaagct cattgacggc
    11281 cgtttcccgg aatatggtcg ggtgattcca gcgaatccaa cgaagcaggt cgaagcggat
    11341 cgagaaggat tgcgccaggc gctgcaacgg accgccattc tctcgaatga gaagtatcgc
    11401 ggagttcgca tcaatttgaa gtcagatctc gtcacgatcc aggcacataa ccccgaacag
    11461 gaagaagccg aggatcaggt cgaggtcacg tacacgggcg atgaagttga gatcggcttc
    11521 aatgtcacct acctcctcga cgcgctcgcg gctgtggatt ccgacaaggt catcctcgga
    11581 cttaccgacg gcaatagcag ttgcctggtt cgcgcggccg gcacgaccaa ctgcaagtac
    11641 gtcgtcatgc cgatgcggct ctaaagctcc cagggcgttg cggttccgat gcctctcgca
    11701 gagctgcgcg tcgagaacct ccgctgcatc gagtccgcgg tcctcgagtt ttcgcccgag
    11761 ctgaatctca tcgccggcga aaatggcgcc ggcaaaacct cgattctcga ggcgatcttc
    11821 ctgcttgggc gtggacgttc gttccggacc aggagcagcg agcggctcat tcgttaccac
    11881 cagccgaaac tcaccgtatt cggccgtacc gacgattcgc caaccaggca ggccggaatc
    11941 gaagtcgccg cagatggcgg cacacgcgcc cggatagacg gggaggcagc cacgtcgctg
    12001 ctcgagctct ccggagtcct gccggtccag gcgatagatc ctgaaatcca caagcttgtg
    12061 gaccaggggc cggaacggcg ccgccggtgg ctcgactggt tggtgttcca cgtggaacca
    12121 agttttggca tccagtgggc caggtacaac cgggcactca aacaacggaa tgcgtccctg
    12181 agggtcgggg gagagagctc cgcttgggat gccgagctga tccgaaatgg catcgccatt
    12241 actgaatcga ggcagcgcgc actcagccgg ctcgtcccgt gcctcgaaca gacctttgag
    12301 cgcttcgggg gactggacgt gtccgtcggc ttcaccacgg gctggctggc tggcacttcg
    12361 ttcgaggaag cactgcgcac gcatgcggag cgggatcgcg ctcgaggcac cacgaccgca
    12421 ggcccccaca gggccgatgt aactcttcgt cggaaccagc ggattgcacg cgaaactctt
    12481 tcacgtgggc agcagaagct cacggccgta gccatgatcg tgtcgcaact gcggcttctg
    12541 caggaggagc tcaacctgcg cgcggtactg cttctggatg acccggcggc agaactcgac
    12601 gataaaaacc ttcagcgatt gttcgaagaa ctctcggctc ttcggtgcca aatgatcgcc
    12661 acttcgctca cgcaagaaac cgcgcttttt caagcaccta aagcgacgtt tcacgtggaa
    12721 cagggaaggg ttatccgggt ataattcgcg cccgttttcc cgagtcagtc atggccaacg
    12781 acgacgtcta cgactccagc aaaatcaagg tcttaaaggg tcttgatgca gtgcgcaaaa
    12841 gacccggcat gtacatcggt gacaccgatg acggcacagg ccttcaccac atggttttcg
    12901 aggttgtgga taacgccatc gacgaggcgc tggcgggcca ttgcgaccgc atcgtcgtca
    12961 ccatccacgc tgacgaatcc gtcaccgtct cggacaacgg tcgcggcatt ccgaccgaca
    13021 tccatcccga agagggccgt tctgcagccg aggtcatcat gaccgtcctg cacgctggcg
    13081 gtaagttcga tgacagctct tacaaagtct ccggcggcct gcacggcgtc ggcgtctcgg
    13141 tcgtcaatgc gctttccgac cacctctggc tgaccatcaa tcgggatggc aaggcccaca
    13201 agatggaatt ccgcctcggt gaccccgtgg cgcccctggc catcgccggc gataccaccg
    13261 agaagggcac ggtcgtccgc ttcaaggcca gcccgaccat cttcacgaac atcacgttcg
    13321 actacgagat cctcgccaag cggcttcgcg aactctcgtt cctcaactcg ggcgtgcgaa
    13381 tcgagctcat cgacgagcgc gaagccaaga acgacgtgtt ccagcacgaa ggcggactgc
    13441 aggagttcgt caaacacctc aaccgttccc gcaccggcat ccacccctct gtgttctggt
    13501 tccgcacgca ggaaggcccc atcggcgtgg aagtggcgat gcagtggaac gactcgtaca
    13561 ccgagtccat gttctgctac acgaacaaca tcccgcagaa agacggcggc acccatctct
    13621 ccggtttccg cgcggcgctc acacgcacgc tgaacgacta catcgaaaaa gagatggcgg
    13681 cgaaaaagga gaaggtcccg accacgggtg acgactcccg cgaaggcctc acggccatcc
    13741 tctcggtcaa actacccgac ccgaagttct cttcgcagac caaggaaaag ctggtctcgt
    13801 cggaagtgaa gggcgtggtc gagtccgcgg tcaccgagaa gctttccgaa ttcttgttag
    13861 agcatccgaa cgaagcgaag gccatcgtcg gcaagatcat cgacgctgcg cgtgcacgcg
    13921 aagccgcgag aaaagcccgt gaaatgacgc gccgtaaagg cgcgctcgac atcgccggac
    13981 ttccgggaaa actcgccgac tgccaggaaa aagatccggc gctgtgcgaa atcttcatcg
    14041 tcgagggtga ctcggcaggc ggctccgcca agcagggccg tgatcgccgc acgcaggcca
    14101 tcctgccgct caagggaaag atcctgaacg tcgagaaggc gcgcttcgac aagatgctgc
    14161 agtccgccga agtcggcacg ctgatcaccg cgctcggctg cggcatcggg cgcgacgagt
    14221 tcgacatcaa caagctgcgc tatcaccgca tcatcatcat gacggacgcg gacgtcgacg
    14281 gttcgcacat ccgcacgctg ctgctcacgt tcttctaccg gcagattccg ctgctcatcg
    14341 agcgcgggca catctacatt gcccagccgc cattgttcaa agtgaagcgc ggcaagcaag
    14401 aggtctacgt caaggacgag ctcgagctca acaccatgtt gctggcctcg gctctcgacg
    14461 acgcggcgct gcacgtcaac agcgcggcac ccgcgctctc gggccctggt ctgcaggggc
    14521 tcgcacgcca gtacatggaa gtgcaggcca tcatcaagcg ctgggctcgt cgctacgacg
    14581 aacgattgct cgaccagctc atctacatgc cggcggtcag cgccgccgag ttcgaacgcg
    14641 ccgactggat gcgtgactgg actcgcgatc tcgccctgca tctgaacgga ctgtccgaca
    14701 gcccgcgcag ctaccgcgtc gagctgcgcc aggtcgacgg ccaccccaga cgcgtgctcg
    14761 tcagcaagac cgaacatggt tcgatcaccg agaaggcgct gcccagggaa ttcttcgaat
    14821 cggccgaata caggcgcatc gtcgagctc
//
LOCUS       DASQJS010000160         5576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2288349, whole genome shotgun sequence.
ACCESSION   DASQJS010000160 DASQJS010000000
VERSION     DASQJS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2288349"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VEW08_06765"
     CDS             complement(<1..180)
                     /locus_tag="VEW08_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40471.1"
                     /translation="MAARYYTHFVTSAEPAHSSEWSGVIELTHPLGERRDSKELRRLL
                     AANFEIDAEDIKILQV"
     gene            291..788
                     /gene="greB"
                     /locus_tag="VEW08_06770"
     CDS             291..788
                     /gene="greB"
                     /locus_tag="VEW08_06770"
                     /inference="COORDINATES: protein motif:HMM:NF002506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HYJ40472.1"
                     /translation="MSRYRPTEKPGSQFVTPAGAARLKQELDELWRVERPRVTQAVSE
                     AAAQGDRSENAEYTYGKRRLREIDRRVRFLRKRLDGMTVVDKPPSDPKRVFFGAWVVL
                     EDDDGKQTKYRIVGPDEFDMADGYISMDAPLARALMRKGLDDEVKVALPGGERTYVIV
                     EISYK"
     gene            862..1386
                     /locus_tag="VEW08_06775"
     CDS             862..1386
                     /locus_tag="VEW08_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYJ40473.1"
                     /translation="MHLTDTMQRYIVHWGEMGSRWGMNRSVAQIHALLYLSEKPLNAD
                     EIGETLGIARSNVSTGLKELASWELVQVTHVLGDRRDFFAAQHDTWEVIRVILEGRKR
                     REIDPTLAALRECAAQLKTDKETPEAVRDRIVTQLEFLETVTRWYDSVKRLPRKTLLK
                     MMRMGERIAKVIGD"
     gene            1397..2059
                     /locus_tag="VEW08_06780"
     CDS             1397..2059
                     /locus_tag="VEW08_06780"
                     /inference="COORDINATES: protein motif:HMM:NF025135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HYJ40474.1"
                     /translation="MTQATTFTPPQTHPLTNDGVGDTARHGVREVLGTEAWNRLPEAV
                     RERFADTAVAASYAGAFEIVRASLLGRVFAWLGTLFGTPVAPRAALNVEAHVRVRPDA
                     NGVAWDREYRWADGARDLVRSTKVVDEHGKLIEKLPARLCMPLDTFEEGGVLHFVSRG
                     YYFDLGIRLWLPALLSPGVTHVEHIDLGHGWFRFTMTVMHPLFGEVFFQTGRFSASEE
                     LP"
     gene            2056..3906
                     /locus_tag="VEW08_06785"
     CDS             2056..3906
                     /locus_tag="VEW08_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HYJ40475.1"
                     /translation="MTLVFSLLTVQTLLGALDTLWNHEFAERLPARRAARHELALHAT
                     RQFIYGFLFVALAWREWHGAWAALIAACLLLEVGVTIADFVVEDRTRRLPAFERVLHT
                     VLTLLFGVLLMAIAPVLLDWWRLPTGVFATDYGGFSVLLALFAVGMTAWGVRDAIAAL
                     SHFRPAEWLRDPIESAPQASGRGILVTGATGFIGGHVVRTLRKRGDVVWVWTRDADRA
                     LARFGPNVHVVTKLGDIPPAARIDAIVNLAGAPVIGMPWTQARRQLLIDSRVKTTQAV
                     LDWTATRREPPRVLVSASAIGFYGPADDAWLTEDSPPTAVFQSRLCVEREAAANAAEA
                     QGIRAVNLRIGLVLGKDGGILPQLARPAKFGMAARIGDGRQWMSWIHIVDLLRIVELA
                     IDAPNLRGAINAVAPAPERQGDFQRALARSLRRPYFMRIPAGALRFALGEMAELLVLG
                     QRVAPRRLLNAGFEFRHYTLASALRDLVPDRVGAPALRAADSNCEVWFNGDCPVCSME
                     IGSYQKLARRRDLPVRFHDAMRVAQPLSAYGLRREHLERRLYLRDEHGHIQSGFRAVL
                     ALWARLPGYRHLVGVFSWPPLLALCETIYDHALAPGLAYWARVRQTGART"
     gene            3903..4274
                     /locus_tag="VEW08_06790"
     CDS             3903..4274
                     /locus_tag="VEW08_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40476.1"
                     /translation="MNESFALTSVIVISIALSSATVFAIYHPLHKLLEAVCPLGFTAE
                     FWTRAAVTVIYLLPLWVVLVFGLPDLQRVDYFSAGEIARRALAAASFALVGIVIATGL
                     RLSTLSPPAGYDSSFKDRAVR"
     gene            complement(4296..>5576)
                     /locus_tag="VEW08_06795"
     CDS             complement(4296..>5576)
                     /locus_tag="VEW08_06795"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HYJ40477.1"
                     /translation="GDDRGRKVAESYGVKFVVLPLTRDNFRRELDQYIGRGDFVLNVS
                     VDVSSIALIKFCWSKGAMYLDTCIEPWPGGYTDATIPHAKRTNYALREEALGLRANNA
                     RAPTAVITHGANPGLVSHFVKQALLNIAADTGVDAGSPRSKDDWAQLSRTLGIKAIHI
                     AERDTQLSSKPKEFNEFVNTWSVDGFVSEGQQPSELGWGTHEKNFPRDGKRFDFGCGA
                     AIYLMQPGAATRVRTWTPKAGHFFGFLITHGEAISIADYLTVRQSSEAVYRPTVHYSY
                     HPSDSAIVSLHEFAGRNWHLQDRQRILLDEITSGVDELGVLLAGHKKNAYWYGSRLSI
                     EEARKLAPHNNATSLQVTVSCLSGMIWAMENPNAGLVEPDEIDYVRNLEICMPYLGAV
                     AGAYTDWTPLHDRERLFPEDLDKTDPWQFKNVRV"
BASE COUNT          943 a         1814 c         1804 g         1015 t
ORIGIN      
        1 cacctgcaag atcttgatgt cctcggcgtc gatctcgaag ttcgcggcca gcaagcggcg
       61 cagttccttc gagtcccggc gttcacccag cgggtgtgtc agctcgatta cgccgctcca
      121 ctcgctcgag tgcgccggct cagcgctcgt gacaaagtgc gtgtaataac gggctgccat
      181 ggggataaag cgtaaattga ccaccccaaa acagctgtga gccgctgcac agaacccccc
      241 ggcagactgc cgccgcgatt gagaccccca tcacaactga tttaaaagtc gtgagtcgct
      301 accgccctac agagaaaccc ggatcgcaat tcgtcacccc cgcgggtgca gcacgcctca
      361 aacaagagct ggacgagctg tggcgggtcg agcgcccgcg ggtcactcag gcggtctccg
      421 aagccgccgc acagggcgat cgctccgaga acgccgaata cacctacggc aaacgccgcc
      481 tgcgtgaaat cgaccggcgc gtgcgattcc tgcgcaagcg gctggacggc atgaccgtgg
      541 tcgacaagcc gccctcggat ccaaagcggg tatttttcgg cgcctgggtg gtactcgaag
      601 atgacgatgg caaacaaaca aagtaccgca tcgtcgggcc cgatgaattc gacatggctg
      661 atggctacat cagcatggac gcgccgctgg cgcgcgcgct catgcgcaag ggactggatg
      721 acgaggtgaa agtggccctg ccgggcggcg agcgcacgta cgtgatcgtc gaaatttcct
      781 acaaataagt acaaccgagg cttgccgtgg ggccgcaatg gatgtaattt ctgtaacgac
      841 agaaattaca ggcggcacga tatgcacctc accgacacca tgcagcgtta catcgtccac
      901 tggggcgaaa tgggcagccg ctggggcatg aaccgctccg tggcgcagat ccacgccctg
      961 ctctatctct ctgaaaaacc cctcaacgcc gatgagatcg gcgagacgct gggcatcgcc
     1021 cgctccaacg tcagcaccgg cctgaaggaa ctcgcaagct gggaactggt gcaggtcacg
     1081 cacgtgctcg gcgaccggcg cgacttcttc gcggcccagc acgacacctg ggaagtcatc
     1141 cgcgtgattc tcgaaggacg taaacgccgc gagatcgatc cgacgctcgc cgcgctgcgc
     1201 gaatgtgcgg cgcagctcaa gaccgacaag gagacgccgg aagccgtacg cgacaggatc
     1261 gtcacgcagc tggagtttct cgagacggtg acgcgctggt acgactccgt gaaacgcctg
     1321 ccacgcaaga cgctgctcaa gatgatgcgc atgggcgagc gcatcgcgaa ggtcatcggc
     1381 gactgacggc acccatatga cgcaggccac cacattcacg ccgccccaga ctcaccctct
     1441 gacgaacgat ggagtcggtg acacggcccg ccatggcgtg cgtgaagtgc ttgggaccga
     1501 ggcctggaat cgcctgcccg aagcggtccg cgagcgcttc gccgataccg ccgtcgcggc
     1561 cagctatgcg ggcgcgttcg aaatcgtccg cgcgagcctg ctcgggcggg tgtttgcgtg
     1621 gctgggcacg ctgttcggca caccggtggc gccgcgcgct gcgctcaatg tcgaggcgca
     1681 cgtgcgggta cggccggacg cgaacggcgt tgcctgggat cgcgaatatc gctgggccga
     1741 cggcgcacgc gatctcgttc gatccacgaa ggtggtcgat gaacacggaa aactcatcga
     1801 gaaactgccg gcgcgcctgt gcatgccact agatacattc gaagaaggcg gcgtccttca
     1861 cttcgtcagc cgcggctact attttgacct cgggatccgg ctgtggctgc cggctttgct
     1921 ctcccccggc gtcacccacg tagaacacat cgatctcggc cacggctggt tccgcttcac
     1981 gatgaccgtg atgcatccgc tgttcggcga agtgtttttt cagaccgggc gcttcagcgc
     2041 cagcgaggag ttgccatgac actggtcttt tccctgctca ccgtccagac gttgttaggt
     2101 gcgctggaca cgctctggaa ccacgaattc gcagagcgcc tgccggcgcg ccgtgccgcg
     2161 cgccacgagc tggccctgca tgccacgcgc cagttcatct atggctttct cttcgtggct
     2221 ctggcctggc gcgagtggca cggcgcgtgg gcggcgctca tcgccgcgtg cctgctgctg
     2281 gaagtgggcg tcaccatcgc ggacttcgtg gtcgaagatc gcacccggcg gctgcctgct
     2341 ttcgagcgcg tgctgcacac cgtgctgacg ttgttgtttg gcgtgctgct catggcgatc
     2401 gcgcccgtgc tgctcgactg gtggaggctg cccaccggcg tgttcgccac cgactacggt
     2461 ggattctccg tgctgctcgc cctgttcgcg gtcgggatga ccgcctgggg cgtgcgcgat
     2521 gccatcgcgg cgctctcaca cttccggccg gccgaatggc tgcgcgaccc gatcgagagc
     2581 gcaccacagg cttccggccg cggaatcctc gtcaccggcg cgaccggctt catcggtggc
     2641 cacgtggtgc gcacgttgcg caagcgcggc gacgttgtct gggtatggac tcgcgacgcc
     2701 gatcgcgccc tggcgcgttt cgggccaaac gtgcatgtgg tcacgaagct gggtgacatt
     2761 ccgccggccg cgcgcatcga cgcgatcgtg aatctggcgg gcgctccggt catcggcatg
     2821 ccatggaccc aggcgcgccg tcagctgctc atcgacagcc gtgtcaaaac cactcaggcg
     2881 gtgctggact ggaccgccac gcgccgcgag ccgccccggg ttctggtgag cgccagcgca
     2941 atcgggttct atggaccggc cgacgatgcc tggctcaccg aagactctcc gcccacggcc
     3001 gttttccagt cacgcctgtg cgtcgaacgc gaagcggccg cgaacgcggc ggaagcgcag
     3061 ggcatccgcg ccgtgaacct tcgcatcggg ctcgtgctcg ggaaagacgg tggaatcctt
     3121 ccgcaactcg cgcgacccgc gaagttcggc atggccgcga gaatcggtga cggccggcag
     3181 tggatgtcct ggattcacat cgtcgacctg ctgcgcatag tcgagctcgc cattgacgcg
     3241 ccgaatctgc gtggcgcgat caacgcggtg gcgcccgcac ccgagcgcca gggagacttt
     3301 cagcgcgcgc tggcgcgcag cctgcgccga ccgtacttca tgcgcatccc cgccggggca
     3361 ctgcgcttcg cgctcggcga gatggcggag ttgctggtgc tgggtcagcg cgtggcacca
     3421 cggcgcctgc tgaatgcggg cttcgagttc cgccactaca cgctggcgag cgcgctgcgc
     3481 gatctcgttc ccgatcgcgt gggcgcgccc gcgttgcgcg ccgcggacag caattgcgaa
     3541 gtctggttca acggcgattg cccggtttgt tcgatggaga tcggcagcta ccagaagctc
     3601 gcccggcgtc gcgacctgcc ggtgcgtttc cacgatgcga tgcgcgtggc gcagccgctg
     3661 tccgcctatg gattgcgccg cgagcatctg gagcgccgcc tctacctgcg cgacgagcac
     3721 ggccacatcc agagcggatt tcgcgccgtg ctcgcgctct gggcgcgact ccccgggtac
     3781 cgccacctgg tcggggtttt ttcctggccg ccattgctcg cactctgcga aacgatttac
     3841 gaccacgcct tagcaccagg actcgcgtat tgggcgcgcg tccgccagac aggagcaaga
     3901 acatgaacga gtctttcgcc ctcacctccg tcatcgtgat ttccatcgcg ctcagcagcg
     3961 ccacggtgtt cgcgatctac cacccgctgc acaaactgct cgaggcagtc tgcccactcg
     4021 gctttaccgc cgaattctgg actcgcgccg cggtgacggt gatctacctg ctgccgctgt
     4081 gggtggtgct ggtgttcggc ttgcccgacc tgcagcgcgt ggactatttc tccgccggcg
     4141 agatcgcgcg ccgcgcgctg gccgcggcgt ccttcgcact ggtcggaatc gtcattgcca
     4201 cgggcctcag gttgtccacc ctgagcccgc cggctggcta cgactcttct ttcaaagacc
     4261 gggcggtgcg ctgaactgct cgcgtcgccc gagcctcaca cgcggacgtt cttgaactgc
     4321 cagggatcgg tcttgtcgag gtcttccggg aacagccgct cgcgatcgtg cagcggcgtc
     4381 cagtccgtgt aagcgccggc gaccgcgccg agatacggca tgcagatttc gagattgcgc
     4441 acgtagtcga tttcgtcggg ctcgacgaga ccggcgttcg gattctccat tgcccagatc
     4501 attcccgaca ggcacgacac cgtgacctgc aggctggtgg cattgttgtg aggcgcgagc
     4561 ttgcgagctt cttcgatgga cagccgcgag ccgtaccagt acgcgttctt cttgtggccg
     4621 gcgagcagca cgccgagttc gtcgacgccg ctggtgattt catcgagcag gattcgctgg
     4681 cgatcctgca ggtgccagtt gcggccggcg aattcgtgca gcgagacgat ggccgaatcg
     4741 ctggggtgat acgagtaatg cacggtcggc cgatacacgg cctcggagct ctgccgcacc
     4801 gtgaggtagt cggcgatcga aatggcttcg ccgtgcgtga tcaggaagcc gaagaaatga
     4861 ccggccttgg gtgtccaggt gcgcacgcgc gtggccgcgc ccggctgcat gaggtagata
     4921 gccgcgccac agccgaagtc gaagcgcttg ccgtcgcgcg ggaaattctt ttcgtgtgtg
     4981 ccccagccga gctcggatgg ctgctggcct tcgctgacga agccgtcgac cgaccaggtg
     5041 ttgacgaatt cgttgaactc cttcggcttc gacgagagct gtgtgtcgcg ctcggcgatg
     5101 tggatggcct tgatgccgag cgtgcgcgac agttgcgccc agtcgtcctt tgacctgggc
     5161 gaaccggcgt cgacgcccgt atccgcggcg atgttgagca gcgcctgctt gacgaagtgc
     5221 gagaccaggc ccgggttggc cccgtgggtg atgaccgccg tcggcgcgcg tgcattgttc
     5281 gcgcgcaacc ccagcgcctc ttcgcgcagc gcgtagttgg tgcgcttggc gtggggaatg
     5341 gtggcgtcgg tatagccacc cggccacggc tcgatgcagg tgtcgaggta catcgcgccc
     5401 ttggaccagc agaacttgat caacgcgatc gaagacacat cgactgacac gttgagtacg
     5461 aagtcaccac ggccgatgta ctgatcgagc tcgcggcgga agttgtcgcg cgtcagcggc
     5521 aggaccacga atttcacgcc ataagattcg gcgactttcc ggccgcgatc gtcacc
//
LOCUS       DASQJS010000161         8368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_88493, whole genome shotgun sequence.
ACCESSION   DASQJS010000161 DASQJS010000000
VERSION     DASQJS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88493"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..2980)
                     /gene="polA"
                     /locus_tag="VEW08_06800"
     CDS             complement(194..2980)
                     /gene="polA"
                     /locus_tag="VEW08_06800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HYJ40478.1"
                     /translation="MNSRPDLVLVDGSSYLYRAFHALPPFSNSRGEPTGAVFGVLNML
                     AKFLKDYDPERIAVVFDAPGKTFRDELFAEYKAHRPPMPDDLRSQIEPLFEAVRGMGL
                     PILRETGVEADDVIGTLACAAAKQNLSVLISTGDKDMAQLVSPKITLINTMSGTVLDR
                     EGVKTKFDVFPEQIIDYLALVGDSSDNIPGIDKVGPKTAAKWLGKYSTLDALVANAAS
                     VEGKVGENLRAGLATLELARKLATIRTDVPLPVTLQELKRNPPDIDALRVLYSRLELR
                     SLLKQLDGSGAPGGPAPTAPDAPAATGQPAIVGAPAAAGISDGAQIIAAAPRLYETIT
                     TEAQLDAWLKKLEGAELFAFDTETTSLEYMHASIVGVSFAISPGVAAYLPLRHDYAGA
                     PDQLDRDSALAKLKPLLESEKHAKVGHHLKYDAHVLANHGIRLAGMRYDSMLESYVWN
                     SVAIRHDMDSAAERYLGIRTIHYEDVAGKGAKQISFSQVPVDKAAEYSSEDSDVTLRL
                     HQVLWPQISSVPALKTLYEECEQPLVPVLLRMEEHGVLLDRQMLRTQSTELGKRLMEL
                     LGEAHKEAGAPFNLDSPKQLGNILFEKMQLPVVRKTPTGQPSTAEDVLEELAETYALP
                     KLILEHRNLSKLKSTYTDKLPEQINARTGRVHTSYHQAVAATGRLSSQDPNLQNIPIR
                     TPEGRRIRQAFIAPDGHVLLAADYSQIELRIMAHLSGDEGLLRAFASDLDIHQATAAE
                     VFGATLDTVTSDQRRSAKAINFGLIYGMSAFGLAKQLGIPRNAAQEYIDLYFARYPGV
                     KRYMEETRAQAKAQGYVETVFGRRLYLPDINARNKQFQQAAERAAINAPMQGTAADII
                     KRAMITVDAWCGSGERGARIIMQVHDELVLEVETARIAEVTDAVRQRMMAAADLRVPL
                     KVDVGTGSNWDEAH"
     gene            complement(2997..3284)
                     /locus_tag="VEW08_06805"
     CDS             complement(2997..3284)
                     /locus_tag="VEW08_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40479.1"
                     /translation="MADVSMRRITLLCLLLTALPGCGKKGPLYLPDAAGEVVTRPAEV
                     PPPAESTEAPNSPQTVDSQPSPPAAAPEVSAPESNEESPKKDKGGTPPPPK"
     gene            complement(3277..3744)
                     /locus_tag="VEW08_06810"
     CDS             complement(3277..3744)
                     /locus_tag="VEW08_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40480.1"
                     /translation="MASQVNQHPLSGYTHMMYALHALAGFIGVTSGATIVGAFVFGLP
                     SIIAILMNYARRDAVRGSWLESHFLWQARTFWTAVVLGAGLFCVALVLSAFGLVSLAS
                     VPLTGGVGAAGAGAGFAGALVALTLGALLAGIWILYRVVRGWLALRDGKVMHG"
     gene            complement(3729..4160)
                     /locus_tag="VEW08_06815"
     CDS             complement(3729..4160)
                     /locus_tag="VEW08_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HYJ40481.1"
                     /translation="MAIWFGNPTIDDARASHVGLLASHLGIEFIEWGEDFLRGTMPVE
                     PRTRQPMGYLHGGASMALAETLGSVAANYVVDYAKFRCLGQEINANHLRPVTDGLVIG
                     TARPMHLGSRSQVWSIEIRDPRERLVCISRLTVAVVEWHPK"
     gene            complement(4178..5362)
                     /gene="argH"
                     /locus_tag="VEW08_06820"
     CDS             complement(4178..5362)
                     /gene="argH"
                     /locus_tag="VEW08_06820"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HYJ40482.1"
                     /translation="MSRLWDKGVDLDQRVLAYTAGDDYALDERLVQYDVRASIAHAEM
                     LNAQKLLADADLKAIREGLIAIGDEHAAGHWKIELADEDGQTALEKRLTARIGAAGGR
                     VHLGRSRNDQVLTAVRLYLLDAACELAAAAEGCAAALDALAAREGTIALPGYTHMQQA
                     MPSSVALWAQGFAAELRDDAQGLGSMTRRASRSPLGSAAGYGTPGLPLDRELTRSKLG
                     FDSVHSPVTAVQLARGKAEAQLLFECTLLMQDLGRLAADLLLFYTQEFAFVTLPASFT
                     TGSSIMPQKRNPDVFELVRGRSAVAQSCLSEVLGIFAKLPSGYQRDLQLIKPPLFRGI
                     DLAFATASIMSIAIDGVRFRSDQIKLDPAIHAAEEANALVAREGIPFREAYRRIGEKF
                     RK"
     gene            complement(5369..6304)
                     /gene="argB"
                     /locus_tag="VEW08_06825"
     CDS             complement(5369..6304)
                     /gene="argB"
                     /locus_tag="VEW08_06825"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HYJ40483.1"
                     /translation="MIMHRPDPSAAIRSLRSAAPYIRMYKGKTFVVKAGGGVFADAAA
                     VRGLIEQIAILHYFGVRVVFVHGGGPQLTEITEALGVPTRMVQGRRVTDQKAIDATSM
                     VLNGLINTRLLAFCREMDIDAVGISGVDAGLIRAHKRPPVRLADTGETVDYGFVGDID
                     SIDTTVIKKLLDNGLMPVVSPLSADDQGTLLNINADTVAAAIGAALSAEKLMLCTGAP
                     GILERVDDPSSIISYTDLNGLKRLREEKKIVDGMLPKAKAIEDAIRGGVRRVHVMSYK
                     SPEGILAEVFTNEGAGTLIVADVNALSPAEQQVEK"
     gene            complement(6333..7340)
                     /locus_tag="VEW08_06830"
     CDS             complement(6333..7340)
                     /locus_tag="VEW08_06830"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HYJ40484.1"
                     /translation="MNNFLDLADLQREQVIELLELAERLAQRPEPSALAGKILGLLFF
                     NPSLRTLASMQAAMARLGGNSFVISAGQGTWQLETRRGAVMNGAPAEHIREGVPVLAH
                     YCDALGIRAFAGGADLEADLNETSFKEMVGLVGKPVINMESAANHPCQALADWKTLDD
                     LAVPRKARFVLSWVNHPRALPLAVPAATVHMAAMRGMDVVVLRPEGFALPPAIMQKAA
                     AAAAAAGGSVSETNDRDEALTGAHVVYAKEWGSTSHYGNAEIDARQRTSLADWCVRDT
                     WFSRAAVDCKLMHCLPVRRNVAVADEVLDGRRSVVQREAYNRLVVQTAVLHRLLSTPQ
                     H"
     gene            complement(7337..>8368)
                     /locus_tag="VEW08_06835"
     CDS             complement(7337..>8368)
                     /locus_tag="VEW08_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYJ40485.1"
                     /translation="YGGHAVASLGYGHKGWTKALADQAAQMNFQTNAVPMEIRARAAQ
                     KLLKFSRLPFDSVFWINSGAEANENAFKMAFKMRPGRTHVAAVEQSFHGRTAACASTT
                     WQAPQKWFFLPRAPFDVSWIKRRDVADIANHVTENTAAVIVEPVQGVGGAFDMGQEFL
                     AALRKRCDETGALLIFDEVQCGVGRTGAPFAANYYGVMPDMITTAKALGNGFPVSALL
                     LTPAITASLKLENLGTTFGGGPMACAVAEATLSAIEKEDLLANVRRVSAYLTKTCAVG
                     PVVGFQGAGFLAGLKMNKPAKDIQKALLSRDILAGTAGDPSILRLLPAFILKEEHIDR
                     LRAALLELA"
BASE COUNT         1426 a         2638 c         2798 g         1506 t
ORIGIN      
        1 ccgcagcgaa cttcatgttc atgagagacc tctccagtgt tttagggcta acgattagag
       61 cgcaggttaa cttaaccaca cctgaactat ctgccgccat ccgccgcaat gtgacgcgct
      121 gcacattagg gcgccctggc agcgccacgg gacccggttg gcctatccac cgggcagtta
      181 ggtccaacta gtcctagtgc gcctcgtccc agttagaccc ggtcccgacg tccactttga
      241 gcggaacgcg caggtcggcc gcggccatca tgcgctgtcg gaccgcgtcg gttacttcgg
      301 cgatcctcgc ggtctcgact tccaatacca gttcgtcatg cacctgcatg atgatgcgcg
      361 cgccgcgctc accggacccg caccaggcgt ccaccgtgat catggcgcgc ttgatgatgt
      421 cggccgcggt gccctgcatg ggcgcgttga tggccgcgcg ttccgccgcc tgctggaact
      481 gcttgttgcg cgcgttgatg tccggaaggt agaggcgccg cccgaacacg gtctcgacgt
      541 acccttgcgc cttcgcctgc gcgcgcgtct cttccatgta gcgcttcacg cccggatatc
      601 gcgcgaagta caggtcgatg tactcctgcg ccgcattgcg cggaatgcct aactgcttgg
      661 cgaggccgaa tgcgctcatg ccgtagatga gaccgaagtt gatggccttg gcggagcgcc
      721 gctggtccga ggtgactgtg tcgagcgtgg cgccgaaaac ttcggcggcg gtggcctggt
      781 ggatatcgag atcgctggcg aaagcgcgca gcagaccttc atcaccagac agatgcgcca
      841 tgatgcgcag ctcgatctgc gaataatccg ccgccagcaa cacatgtccg tccggcgcga
      901 tgaaggcctg gcgtatgcgc cgtccttccg gcgtgcggat cgggatgttc tgcaggttgg
      961 gatcctgcga agacaatcgg ccggtggctg ccaccgcctg gtggtaactg gtatgcacgc
     1021 gtccggtgcg ggcattgatc tgctcgggta gtttgtcggt gtaggtggac ttgagcttgg
     1081 acagattgcg atgctcgagg atcagcttgg gcagcgcgta ggtttccgcg agctcctcga
     1141 gcacatcctc cgctgttgat ggctgccccg tgggagtctt gcgaaccacg ggcagctgca
     1201 tcttttcgaa caggatgttg ccgagctgct tgggtgaatc gaggttgaat ggggcgcccg
     1261 cttccttgtg cgcctcgccc agcaattcca tcagccgctt gccgagctcc gtactctggg
     1321 tgcgcaacat ctggcggtcg agcaatacgc cgtgctcctc catgcgcagc agcacgggca
     1381 cgagtggttg ctcgcattct tcatagagag tcttgagcgc ggggacactc gagatctgcg
     1441 gccacagcac ctggtgcagg cgcagcgtca cgtcggaatc ttcggaggag tactcggcgg
     1501 ccttgtccac cggcacctgg ctgaaggaaa tctgtttcgc gcccttgccc gccacgtctt
     1561 catagtgaat ggtgcggatg ccgagataac gctccgcggc ggagtccatg tcgtggcgaa
     1621 tggccacgct gttccagacg taggactcga gcatggagtc gtagcgcata ccggctagtc
     1681 ggatgccgtg attggcgagc acgtgtgcgt cgtacttgag atggtggccg accttggcgt
     1741 gtttttccga ctcgagcagc ggcttgagtt tcgcgagagc cgagtcgcgg tcgagctggt
     1801 cgggtgcgcc ggcatagtcg tgccgcaggg gcagataggc ggcgacgcct ggcgaaatgg
     1861 cgaacgagac gccgacgatc gacgcgtgca tgtactcgag gctcgtagtc tcggtgtcga
     1921 aggcaaagag ctcggcgccc tcgagcttct tcagccatgc gtcgagctgt gcttcggtgg
     1981 tgatggtctc gtagagacgc ggcgcggccg caatgatctg cgcgccgtcg ctgattccgg
     2041 cggccgcggg tgcgcccacg atggccggct ggccggtcgc ggccggtgca tccggagcag
     2101 tgggagcagg cccgcccggt gcgcccgacc catcgagctg tttgagcagc gaacgcagct
     2161 cgagtcggga gtacaacaca cgcaatgcgt cgatgtcggg cggattgcgc ttgagctcct
     2221 gcaacgtcac cggcagcggc acatcggtgc ggatagtcgc gagcttgcgc gcgagctcga
     2281 gagtcgcgag gccggcacgc aggttttcgc cgaccttgcc ctcgacgctg gcggcgttgg
     2341 ccaccagtgc atcgagcgtc gaatatttcc ctaaccactt cgccgcggtc ttcggcccga
     2401 ccttgtcgat gccgggtatg ttgtcggagg agtcgcccac cagcgcgagg tagtcgatga
     2461 tctgctcggg gaagacgtcg aacttcgtct tgacgccttc gcgatctagc accgtgccgc
     2521 tcatggtgtt gatcagcgtg atcttcggcg agaccagctg ggccatgtcc ttgtcgccgg
     2581 ttgaaatgag cacgctgaga ttttgcttcg cggctgcgca ggcaagcgtg ccgatcacgt
     2641 cgtcggcctc cacgcccgtc tcgcgaagga tcggcaggcc catgccgcgc acggcttcga
     2701 acaaaggttc gatctgggat ctcaaatcgt ctggcatcgg cgggcgatgc gccttgtact
     2761 cggcgaacag ttcgtcgcga aacgtcttgc ccggggcgtc gaagaccacc gcgatgcgtt
     2821 cggggtcata gtccttgaga aacttggcga gcatgttcag cacgccgaac accgcgccag
     2881 tcggttcgcc gcgcgaattg ctgaatggcg gcagcgcgtg gaacgcgcga tacagatacg
     2941 aagaaccgtc gacgaggacg agatccgggc gggagttcat gcgagcggcg ttccgtttat
     3001 ttcggcggag gcggagtgcc gcccttgtct ttcttcggcg attcttcgtt cgactccggc
     3061 gccgagactt ccggagcggc cgccggcggc gaaggctgcg agtccacggt ctgcggagag
     3121 ttcggtgctt cggtgctttc agcgggcggt ggtacttcgg cgggccgtgt caccacctcg
     3181 ccggcagcgt cgggtagata gagcgggccc ttcttgccgc agcccgggag cgcagtcaac
     3241 aggaggcaga gcagggtaat gcgccgcatg gagacgtcag ccatgcatca ccttgccgtc
     3301 gcgcagcgcc aaccagccgc gcacgacgcg atagaggatc cagatgccgg cgagcagggc
     3361 gcccagcgtc aaggccacga gcgcgccggc aaagcccgcg cccgcgccgg cggcgccgac
     3421 gccgccggtc agcggcactg acgcgaggct gaccaggccg aaggcgctca gcaccagcgc
     3481 aacgcagaac aggccggcgc cgagtacgac cgcggtccag aacgtgcgcg cctgccacag
     3541 aaaatgggat tcaagccacg agccacgcac cgcatcgcgg cgcgcgtagt tcatgaggat
     3601 ggcgatgatg gacggcagcc cgaagacgaa ggcgccgacg atcgtggcgc cagaggtgac
     3661 accgatgaag cccgcgagcg catgcagcgc gtacatcatg tgggtgtagc ctgacaaagg
     3721 atgctggttc acttgggatg ccattcgacc acggccacgg taagacggga tatgcatacc
     3781 agtcgttccc ggggatcgcg gatttcgatg ctccagacct ggctgcgcga acccaggtgc
     3841 atcgggcgtg cggtgccaat gacgagtccg tcggtcaccg gacgcaaatg attggcgttg
     3901 atctcctgtc cgaggcaacg aaacttcgcg tagtcgacca cataattcgc cgccacgctg
     3961 cctagagttt cggcgagcgc catcgacgca ccaccgtgca ggtatcccat gggctggcgg
     4021 gtgcgcggct ccaccggcat cgtgccgcgc aggaagtctt cgccccattc gatgaattcg
     4081 atgccgagat gcgacgcgag caaccccacg tggctggcgc gggcgtcgtc gatcgtcgga
     4141 ttgccgaacc agattgccat cgtcactcca ggcgacgtta cttgcggaat ttctcgccga
     4201 tgcggcgata tgcctcgcgg aacgggatgc cttcgcgcgc gaccagcgca ttcgcttcct
     4261 cggcggcgtg aatggcgggg tcgagtttga tctggtcgga acggaaacgc acgccatcga
     4321 ttgcaatcga catgatgctg gcggtggcga acgcgaggtc gatgccgcgg aacaacggcg
     4381 gcttgatgag ctgcaggtca cgctggtatc ccgagggcag cttggcaaag atacccagca
     4441 cttcggagag gcaggattgg gccacggcgc tgcggccgcg caccagctcg aacacgtcgg
     4501 ggttgcgctt ctgcggcatg atcgacgagc cggtcgtgaa tgaagccggc agcgtcacga
     4561 atgcaaattc ctgcgtatag aagagcagca ggtctgccgc gagcctgccc aggtcctgca
     4621 tgagcagcgt gcattcgaac agcagctgcg cttcggcctt gccacgtgcg agctgcacgg
     4681 cggtcaccgg cgagtgcacc gaatcgaaac cgagcttcga acgcgtgagc tcgcgatcca
     4741 gcggcagtcc gggagtgcca tagccggcgg ctgaacccag cgggctgcgc gacgcgcgcc
     4801 gcgtcatgga gccgaggccc tgcgcatcgt cgcgcagttc cgccgcgaac ccttgcgccc
     4861 acagcgccac cgaactcggc atcgcctgct gcatgtgggt ataacccggc agcgcgatgg
     4921 tgccttcacg cgcggccagc gcatcgagtg cggcggcgca gccttcagcg gccgctgcca
     4981 gctcacaggc cgcatcgagt agatagagac ggaccgcggt caggacctgg tcattgcgcg
     5041 agcggccgag atgcacgcgg ccgccggcgg cgccgattcg cgcggtgagc cgcttctcga
     5101 gcgcagtctg gccatcctca tcggcgagct cgatcttcca gtgacccgca gcgtgctcgt
     5161 cgccgatggc gatgagccct tcacgaatcg ccttcagatc cgcatcggcc agcagcttct
     5221 gggcattgag catttcggca tgggcgatgg aggcgcgcac gtcgtactgg accaggcgct
     5281 cgtcgagcgc gtagtcgtcg cccgcggtgt acgcgagtac ccgctggtcg agatcgacgc
     5341 ctttgtccca gagccggctc atgtcctgct acttttcgac ctgctgctcg gccggcgaca
     5401 gcgcgttgac gtcggcgacg atcagcgtgc cggcgccttc gttggtgaac acttcggcca
     5461 ggatgccctc gggcgatttg tacgacatca cgtgcacgcg gcgcacgccg ccgcggatgg
     5521 cgtcttcgat cgccttggct ttcggcagca tgccgtcgac gatcttcttc tcctcgcgca
     5581 ggcgcttgag gccgttcagg tccgtgtacg aaatgatgga agacggatca tcgacgcgct
     5641 cgaggatgcc cggcgcgccg gtgcacaaca tgagcttctc ggccgacagg gccgcgccaa
     5701 tggccgcagc caccgtgtcg gcattgatgt tgagcagcgt gccctggtca tcggccgaca
     5761 gcgggctgac caccggcatc agcccgttgt cgagcagctt cttgatgacg gtggtgtcga
     5821 tggagtcgat gtcgccgacg aatccgtagt ccaccgtttc gccggtgtcc gcaagtctga
     5881 ccggcgggcg cttgtgggcg cggatcaggc ccgcgtctac gccgctgatg cccacggcgt
     5941 cgatgtccat ctcgcggcag aacgccagca ggcgggtgtt gatgaggcca ttgagcacca
     6001 tggatgtggc gtcgatcgct ttctgatcgg tgacgcggcg gccctgcacc atgcgcgtgg
     6061 gcacgcccag cgcctcggtg atttccgtga gctgcggtcc gccgccgtgc acgaacacca
     6121 cacgcacccc gaagtagtga aggatggcga tctgttcgat gaggccgcgc accgcggcgg
     6181 catcggcgaa cacgccaccg ccggctttca cgacgaaggt cttgcccttg tacatgcgga
     6241 tgtagggcgc cgcgctgcgc aggctgcgaa tggcggctga aggatcgggg cgatgcatga
     6301 tcatgcgtga attcccgggt aaggcagagt agttagtgtt gcggcgtcga gagcaggcgg
     6361 tgcaataccg cggtctgcac caccagtcgg ttgtaggcct cgcgctgcac gacgctgcgc
     6421 cggccatcca gcacctcgtc ggcgacggcg acattacgcc gcaccggcag gcaatgcatg
     6481 agcttgcagt ccaccgctgc gcgcgagaac cacgtgtccc gcacacacca gtctgcgagc
     6541 gaggtgcgct ggcgagcgtc gatttcggcg ttgccatagt gggacgtaga gccccactcc
     6601 ttggcgtaca ccacgtgcgc gccggtcagg gcttcatcac ggtcgttggt ctcgctcact
     6661 gagccgcccg cggcggccgc ggcagcggcg gccttctgca tgatggctgg tggaagcgcg
     6721 aacccttcgg gccgcagcac cacgacgtcc atgccgcgca tggccgccat gtgcaccgtg
     6781 gcggccggca ccgcgagcgg tagcgcgcgc ggatggttga cccatgacag aacgaagcgc
     6841 gcctttcgcg gcacggcgag atcgtcgagc gtcttccagt ccgccagggc ctggcaggga
     6901 tgattggccg ccgattccat attgatgacg ggcttgccca cgaggccgac catttccttg
     6961 aacgaggttt cgttcagatc cgcctcgagg tcagcgccgc cggcgaatgc ccggataccc
     7021 agagcgtcgc aatagtgggc caatacaggc actccttccc gtatatgctc cgcaggtgcg
     7081 ccattcatga ccgcgccacg ccgtgtctcg agttgccacg ttccctggcc ggcggagatc
     7141 acgaaggaat tgccacccag ccgcgccatc gccgcctgca tggatgcgag cgtgcgcagc
     7201 gagggattga agaacagcag gccgagaatt tttccggcaa gcgcactcgg ctccggcctc
     7261 tgcgccagcc gctcggcaag ctcgagaagc tcgatgacct gctcgcgctg aaggtccgcc
     7321 aggtccagaa aattgttcat gcgagttcca gcagggcagc ccgcaaccgg tcgatgtgct
     7381 cttccttgag gatgaaggcc ggcagtaaac gcaggatcga ggggtcgccc gcggtgcccg
     7441 cgaggatgtc acgcgacagc agcgccttct gaatgtcctt cgcaggtttg ttcatcttga
     7501 ggcccgccag gaagccagcg ccctgaaagc ccacgaccgg acccaccgcg caggtcttcg
     7561 tgagatacgc cgacacgcgg cgcacgttgg ccagcaggtc ttccttctcg atggcggaga
     7621 gcgtggcttc cgccacggcg caggccatgg ggccgccgcc aaacgtcgtg cccaggtttt
     7681 cgagcttgag cgaggcggta atggccggcg tcagcagcag cgccgaaacc gggaagccgt
     7741 tgcccagcgc cttggcggtg gtgatcatgt ccggcatgac gccgtaatag ttagccgcga
     7801 agggagcgcc cgtgcggccg acgccacatt gcacttcgtc gaagatgagc agcgcgccgg
     7861 tctcgtcgca tcgcttgcgg agggcagcca ggaattcctg gcccatgtcg aaagcgccac
     7921 ccacgccctg cacgggctcg acgatgaccg ccgccgtgtt ctcggtgacg tgattggcga
     7981 tgtcggcgac gtcgcggcgc ttgatccacg agacatcgaa aggcgcgcgc ggcaggaaga
     8041 accacttttg tggcgcctgc caggtggtcg acgcgcaagc ggcggtgcga ccgtgaaagc
     8101 tctgttccac ggcggcgacg tgcgtgcggc cgggacgcat cttgaacgcc atcttgaagg
     8161 cgttttcgtt ggcctccgcg ccggagttga tccagaacac gctgtcgaac ggcaggcggg
     8221 agaatttcag cagtttctgc gccgcgcgcg cacggatctc catgggcacg gcattggtct
     8281 ggaaattcat ctgcgcggcc tggtcggcga gcgccttcgt ccagcccttg tggccgtagc
     8341 ccagcgacgc gacggcgtgt ccgccgta
//
LOCUS       DASQJS010000162         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_89827, whole genome shotgun sequence.
ACCESSION   DASQJS010000162 DASQJS010000000
VERSION     DASQJS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89827"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VEW08_06840"
     CDS             <1..518
                     /locus_tag="VEW08_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYJ40486.1"
                     /translation="HEWAYANDYNPAERRLMPHVSLKERFKKLNIEVELGFTVEQIEK
                     EVQRCLNCDVQTVFETKLCIECDACIDICPVDCLTITQNAEEPALRTQLKAPAKNVTQ
                     ALYVSSALKHTGRVMVKDEDLCVHCGLCAERCPTAAWDMQKSTIQWPHAVDNAAADAA
                     KLPLIASRTGA"
     gene            520..>2259
                     /locus_tag="VEW08_06845"
     CDS             520..>2259
                     /locus_tag="VEW08_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HYJ40487.1"
                     /translation="MPERVNNFAIKIATVNGTGSASANTLLMKSIFRSGVPVMGKNYF
                     PSNIQGLPTWYEIRVTKDSYVARSGKIDIMVAMNAETYTKDVREVVSGGYLVYDSTWP
                     RPALLARDDITVLGVPLAKMCNENFNGVRTRILMKNICYAGVLAALLDLDIAKIRELL
                     AETYAKKPALVDSNMKAIELGYNYTRDNFKCPLPMHVAPGLLGGSKTAGHVLIDGNTA
                     AGLGCVFAGATVGAWYPITPSTSLMDAYKAFADRLRVDPATGKKNFAFIQAEDELAAI
                     GMVLGANWNGARAFTATSGPGLSLMNEFLGLAYYAEVPAVLFDIQRVGPSTGMPTRTQ
                     QCDILEAAYASHGDTKHVCLFPRDPEECFYFTRDAFDLAERLQTPVMVMSDLDIGMND
                     WMCPDLKWDDSYVPDRGKVYSKEEIAKLEKFHRYLDVDGDGIPWRTLPGVHPKGAYFT
                     RGSGHTQYGVYTEDSAEYQVVLDRLTRKFATAKRLVPKPVIEQDAHIDIAVVAYGSSD
                     GAVREALDILAEHGIKTNYMRLRSFPFGDEVEKFLEQQKLVFVVEQNRDGQMRSLLTL
                     ETRVEKAKLRSLLH"
BASE COUNT          474 a          716 c          687 g          382 t
ORIGIN      
        1 tgcacgagtg ggcctatgcc aacgattaca acccggcaga acggcggctc atgccgcacg
       61 tctcgctcaa agagcgattc aagaagctca acatcgaagt ggagctgggt ttcaccgtcg
      121 agcagatcga gaaagaggtg caacgctgcc tcaactgcga cgtgcagacg gtgttcgaga
      181 ccaagttgtg catcgagtgc gacgcctgta tcgacatctg cccggtggat tgcctgacca
      241 tcacgcagaa tgccgaggaa ccggcgctgc gcacgcagct gaaagcaccc gcgaagaacg
      301 tcactcaggc tttgtacgta tcgtcggcgc tgaaacatac cggtcgcgtc atggtcaaag
      361 acgaagacct gtgtgtgcac tgcggcctgt gcgccgagcg ctgccccacc gcggcatggg
      421 acatgcagaa atccacgatc cagtggccac acgccgtcga caatgcagca gccgacgccg
      481 ccaaactgcc ccttattgca tcaagaacgg gcgcctgaga tgccagaaag agtaaataac
      541 ttcgcgatca agatcgcgac ggtcaacggc actggttcgg cgagcgccaa cacgctgctc
      601 atgaagtcca tcttcagaag cggtgtgccc gtcatgggca agaactactt tccctcgaac
      661 atccagggcc tgcccacctg gtacgaaatc cgcgtcacca aggattcgta cgtcgcgcgt
      721 tcggggaaaa tcgacatcat ggtcgcgatg aacgccgaga cgtacacgaa agacgtgcgc
      781 gaggtggtct ccggcggcta cctggtctac gactccacct ggccccgtcc ggccctgctg
      841 gcgcgcgatg acatcaccgt gcttggtgtg ccgctggcaa agatgtgcaa cgaaaacttc
      901 aacggcgtgc gcacgcgcat cctgatgaag aacatctgtt acgccggcgt gctcgcggcg
      961 ctgctcgacc tcgacatcgc gaagatccgc gaattgctgg cggaaaccta cgcgaagaaa
     1021 ccggcgctcg tggattcgaa catgaaggcc atcgagctcg gctacaacta cacgcgcgac
     1081 aatttcaaat gcccgctgcc catgcacgtg gcgccgggcc tgctgggcgg ctcgaagact
     1141 gcgggccacg tcctcatcga cggcaacacc gcggcggggc tgggctgcgt gttcgccggt
     1201 gccaccgtgg gtgcgtggta tccgatcaca ccctcgacgt cgctcatgga cgcgtacaag
     1261 gcgttcgccg accggttgcg ggtcgacccg gccaccggca agaagaactt tgcgttcatc
     1321 caggccgaag atgaactcgc ggccatcggc atggtgctgg gcgccaactg gaatggcgca
     1381 cgcgcattca ccgcgacctc cggcccgggc ctgtcgctca tgaacgaatt cctggggctc
     1441 gcgtactacg cggaagtgcc cgcggtgttg ttcgacatcc agcgcgtggg gccttcaacc
     1501 ggcatgccca cgcgcacgca gcagtgcgac atcctcgagg cggcttatgc gtcgcacggc
     1561 gacaccaagc acgtctgcct gttcccgcgc gatccggagg aatgtttcta cttcacgcgc
     1621 gacgcgttcg atctcgccga gcggctgcag actcccgtca tggtgatgtc ggacctcgac
     1681 atcggcatga acgactggat gtgtcccgat ctgaaatggg acgacagcta cgtgcccgac
     1741 cgcggcaagg tgtacagcaa ggaagaaatc gccaagctcg agaagtttca tcgctacctc
     1801 gacgtggacg gcgacggcat tccctggcgc acgctgcccg gcgtacatcc gaagggcgcg
     1861 tatttcacgc gcggctccgg ccacacgcaa tacggcgtgt acaccgaaga ttccgcggaa
     1921 taccaggtgg tgctcgaccg gctcacgcgc aagttcgcca cggcgaaacg actggttccc
     1981 aagcccgtca tcgagcagga cgcacacatc gacatcgcgg tggtcgccta cggcagctcc
     2041 gatggcgcgg tgcgcgaggc gctcgacatc ctcgcggaac acggcatcaa gactaactac
     2101 atgcggctgc gctcgtttcc gttcggcgac gaagtggaga aattcctcga gcagcagaaa
     2161 ctggtgttcg tcgtcgagca gaaccgcgac gggcagatgc gctcgctgct cacgctggaa
     2221 acgcgcgtgg agaaggccaa gctgcgttcg ctgctgcac
//
LOCUS       DASQJS010000163         4427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2601332, whole genome shotgun sequence.
ACCESSION   DASQJS010000163 DASQJS010000000
VERSION     DASQJS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2601332"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VEW08_06850"
     CDS             complement(<1..179)
                     /locus_tag="VEW08_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HYJ40488.1"
                     /translation="MSDLTPRQTEILRLIQRQINETGMPPTRAEIAGELGFKSPNAAE
                     EHLRALARKGVIALVP"
     gene            225..1847
                     /locus_tag="VEW08_06855"
     CDS             225..1847
                     /locus_tag="VEW08_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYJ40489.1"
                     /translation="MNISRSSVKTSFDVVIVGGGHNGLTCACYLAKSGLRVGVFERRS
                     ILGGAAVTEEFHPGFRNSTASYTVSLLNPKVIADLRLVEHGLKVLERPMQNFLPLPDG
                     RALVAGPESARTTASVRSFSARDAERLPAYYKMLDAAVTLLRSQLLETPPADPRRLRD
                     LWGALQMGRRFRKLDAAVQRDIHELFTRSAGDLLDHWFESDPVKALYGFDAVVGSYAS
                     PYTPGSAYVLLHHVFGEVNGKTGVWGHAVGGMGAITQAMATEARRLGVTLETDAPVKR
                     ILVERGRATGIELEDGRVIQGNAIAANLGPKPLFLKLLADAELPAEFRARIERLRVGS
                     ATFRMNVALSELPKFTAAPRDDGGAHLGSGIILAPTLEYMDHAFADARLTGMSRKPIV
                     EMLIPSTLDTSLAPKGAHVASLFCQHFAYKLPGGRDWRQEKSRAEDLIFATVESYAPN
                     FRASILGHSSLSPLDLEEKLGLAGGDIFHGALGLDQLWAARPMLGYGDYRTPLKSLYL
                     CGSGAHPGGGVTGLPGHNAAREIMRDGVSPNS"
     gene            2031..2342
                     /locus_tag="VEW08_06860"
     CDS             2031..2342
                     /locus_tag="VEW08_06860"
                     /inference="COORDINATES: protein motif:HMM:NF023267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HYJ40490.1"
                     /translation="MKSAIIKVAAVAAFTVLAGCQDLKPLQAEVDNLKQQVSRLTADL
                     AAAKSSADAANAAAQSASSTASGAQSAANQALAAAQASQSCCDATNEKIDRMFRRSIS
                     K"
     gene            2476..3384
                     /locus_tag="VEW08_06865"
     CDS             2476..3384
                     /locus_tag="VEW08_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40491.1"
                     /translation="MSCTGAAAPQNFLFTNSDDLKAQDSLLRRPDIAGAQLVYTWKSL
                     ETARDQYDFSQIERDLAYLTGLDRRLFIQIQDRFFEVRHRNVPAYLLQEPIYRGGLVA
                     QSDNPGENQPEGHGWVAQQWNPAVRERFQRLLSALARKFDGRVWGVNLPESSADVDTK
                     NDKTGFTCDKYFEATLENIAFARKVFSRSHVVQYTNFWPCEWDNDRKYMSRAFEFAKR
                     NRIGLGGPDIVPYRKGQMKNSYPFFNRYKGQLSLVAMAIQEPTLTYTNPETKKKFTRE
                     EFVAFAQDYLGVDVIFWAVSSPWLKD"
     gene            complement(3434..3724)
                     /locus_tag="VEW08_06870"
     CDS             complement(3434..3724)
                     /locus_tag="VEW08_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40492.1"
                     /translation="MNSSDRLRRANALWHAYLAVTGQRFELASFLRDPEVEKQTVAQA
                     LASGDQKLVVLAQDWLRDTGQAVPSVAAARVMPAQAAPAEAAKPSRYLRGVR"
     gene            complement(3740..>4427)
                     /locus_tag="VEW08_06875"
     CDS             complement(3740..>4427)
                     /locus_tag="VEW08_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40493.1"
                     /translation="INAGVTGLACVIMLGERKGYGTDPIFPHNLALTITGAALLWVGW
                     FGFNAGSALSADGRAGLAFANTHCAAATAALTWMAIEWAARGKASVLGIASGAVAGLV
                     AITPAAGYVGIGAALVIGIAASLACFGAVVGLKSWLGYDDSLDAFGIHAVGGVVGALL
                     TGVFASVAIGGVEGNIMAQLIGIGACAAYALVVSLILLMLISVTIGLRVSEEAEVAGL
                     DVSTHGESVE"
BASE COUNT          873 a         1410 c         1356 g          788 t
ORIGIN      
        1 ggcacgagcg cgatgacgcc cttgcgtgcg agcgcccgca ggtgttcttc ggcggcgttc
       61 ggggatttga aaccgagctc gccggcgatt tccgcccggg tgggcggcat gccggtttcg
      121 ttaatctggc gctggatgag acgcaggatt tctgtttggc gcggggtgag atcggacatg
      181 acggattcct ttaaagaaga atcagggctg aggatttgta cactgtgaat atatccaggt
      241 caagcgtcaa aaccagcttc gatgtagtca tcgtcggcgg cggccacaac ggcctcacct
      301 gcgcctgcta cctcgccaaa agcggcctcc gggtcggcgt attcgagcgc cgctccatcc
      361 tgggcggcgc cgccgtgacc gaggaattcc accccggctt ccgcaattcc acggcgagct
      421 acaccgtcag cctgctgaac cccaaggtca tcgccgacct gcgcctggtc gaacacggcc
      481 tgaaggtgct cgagcggccc atgcagaact tcctgcccct gcccgatggc cgcgcgctcg
      541 tcgccggccc ggaaagcgca cgcaccaccg catcggtccg cagcttctcg gcgcgcgacg
      601 cggaacgcct gcccgcctac tacaagatgc tcgacgcggc cgtcaccctg ctgcggtcgc
      661 aactgctgga aacgccaccc gccgatccgc gccggctgcg cgacctgtgg ggcgcgctgc
      721 aaatgggccg ccgtttccgc aagctcgacg ccgcggtgca gcgcgacatc cacgagctgt
      781 tcacgcgcag cgccggggac ttgttagacc actggttcga gtccgaccct gtcaaggcgc
      841 tgtacggttt cgatgcagta gttggcagtt acgcgagccc gtacaccccg ggcagcgcct
      901 acgtccttct ccatcatgta ttcggtgaag tgaacggcaa gaccggagtc tggggacatg
      961 cggtcggcgg catgggagcg ataacgcagg ccatggcaac cgaggcgcgc cggctgggcg
     1021 tcaccctcga aacggatgca ccagtcaaac gcatactcgt tgaacgcggc cgggccacgg
     1081 gtatcgagct cgaagatgga cgcgtgatcc agggcaacgc gatcgccgcc aatctcggac
     1141 ccaagccact tttcctgaaa ctgttagccg atgccgaact gcccgccgag ttccgcgcgc
     1201 gtattgaacg cctgcgcgtg ggctcggcca ccttccgcat gaacgtggcg ctgtccgaac
     1261 tgccgaaatt caccgccgca ccgcgagatg acggcggtgc tcacctcggt tccggaatca
     1321 ttctcgcgcc gacgctcgaa tacatggacc acgcttttgc cgatgcgcga cttacgggaa
     1381 tgtcgcgcaa gcccatcgtg gaaatgctta ttccctcgac gcttgacaca tccctggccc
     1441 cgaagggagc tcacgttgcc tcgttgttct gtcagcattt cgcctacaaa ttgccgggcg
     1501 gccgcgactg gcgtcaggaa aagtcgcgtg ctgaagacct catctttgcg acggtggaat
     1561 cgtacgcgcc gaactttcgc gcgagcattc tcggacattc gtcactgagt cctctcgatc
     1621 tcgaagaaaa actcggccta gcaggcggag atattttcca tggagcgttg ggcctcgatc
     1681 agttgtgggc cgcgcgtccg atgttaggtt acggtgatta ccgcacaccg ctgaaaagcc
     1741 tctacttgtg tggttctggt gcacacccgg gtggcggcgt caccggactt cccggtcaca
     1801 acgcggcgcg agaaataatg cgagatggtg ttagtcctaa tagctgagca gctgacaatc
     1861 ttgctattgt cgccagtggc cgacacgaaa caaatttcag ttgcaaacaa ctcgcgaatt
     1921 tgttgcagtt gcgcccgcct aatagacgtc taccttcact cagatcgcat tcgatgcgag
     1981 ttgatggcga agcggacttg ctcaaccacc aataaagggg atttgtcgtt atgaagagtg
     2041 cgattatcaa ggttgctgcc gttgcagcgt ttaccgtgtt ggccggctgc caggacctga
     2101 agcctctgca agccgaagtc gacaatctca aacaacaggt gagccgtctc accgccgacc
     2161 tcgccgccgc caagagctcg gctgacgctg cgaatgccgc cgctcagtct gcttcatcga
     2221 ccgcgagcgg tgcccagagc gctgccaatc aggcgctggc cgccgcgcaa gcttcgcagt
     2281 cgtgctgcga tgccaccaac gagaagatcg accggatgtt ccgtcgctcg atctcgaaat
     2341 aggttggacg gcgccgcttc ggcgccgctt tccgacgtgg cgcgtaagcg cctgacaaag
     2401 aaacgccgcg cacctgtgcg gcgttttttt tcgcccgcaa tctgcgcagg attcctgcac
     2461 ggactgctgc tctgcatgtc ttgcacgggc gccgccgcgc cgcagaactt cctcttcacg
     2521 aactccgacg acctgaaggc gcaggattcg ctgctgcgca ggcccgacat cgccggcgcg
     2581 cagctggtct acacctggaa gtcgctggaa accgccaggg accagtacga cttttcacag
     2641 atcgagcgcg acctcgccta cctcacgggc ctcgaccgca ggcttttcat ccagatccag
     2701 gatcgtttct tcgaggtgcg gcaccggaac gtgccggcct atcttctgca ggagcccatc
     2761 taccgcggcg gcctggtggc gcagtcggac aacccgggag agaaccagcc ggaaggccac
     2821 gggtgggtcg cccagcaatg gaatccggcg gtgcgcgagc gatttcaaag gctgctgtcg
     2881 gcactggccc ggaaattcga tggccgagtg tggggagtca acctgcccga gagttccgcc
     2941 gacgtcgaca cgaagaacga caagaccgga ttcacctgcg acaagtattt tgaagccacc
     3001 ctcgagaaca tcgcgttcgc gagaaaggtc ttcagcaggt cacatgtcgt tcagtacacc
     3061 aacttctggc cgtgcgaatg ggacaacgac cggaaataca tgtcgcgcgc attcgagttc
     3121 gcgaagcgca accggatcgg cctcggcggt ccggacatcg ttccgtacag gaagggacag
     3181 atgaagaact cctatccgtt cttcaaccga tacaaggggc aactgagcct ggtggccatg
     3241 gcgatccagg aaccgacgct gacgtacacc aaccccgaga cgaaaaagaa gtttacgcgc
     3301 gaagaattcg tcgcgttcgc gcaggactac ctgggagtcg acgtgatctt ctgggccgtc
     3361 tcgagtccct ggctaaaaga ctaatgaacg ccgtcgcttc gacggcgttt tccgacgtgg
     3421 cgcgcagcac cttttaacga acgccgcgca gatagcgcga aggcttggca gcctcggcgg
     3481 gcgcggcctg cgccggcatc acgcgagccg cggccacgga aggcacggcc tggccggtat
     3541 cgcgcaacca gtcctgggca agaaccacca gcttctgatc gccgctcgcc agcgcctgcg
     3601 cgacggtctg cttctcgacc tcgggatccc tcaggaatga ggcgagttcg aaccgctggc
     3661 cggtgactgc aaggtaggca tgccacaggg cgttggcgcg ccgaagtctg tctgagctgt
     3721 tcatgagact catggatggc tactccacac tctcgccatg agtgctgacg tccaggcccg
     3781 cgacctcggc ttcttcgctg acgcgcaaac cgatggtgac cgaaatgagc atcaggagaa
     3841 tcaggctgac gaccagggcg tacgcggcgc atgcgccgat gccgatgagc tgcgccatga
     3901 tgttgccttc gactccgccg atcgcgaccg aagcaaacac gccagtgagc aacgcgccaa
     3961 cgacgccgcc gacggcgtgg ataccgaagg cgtcgagcga atcgtcatat cccaaccagg
     4021 acttgaggcc gacgaccgcg ccgaaacaag cgaggctggc ggcgataccg atgacgagcg
     4081 ccgcaccaat accgacgtag cccgccgcgg gcgtgatggc gacgagtccc gcgaccgcgc
     4141 ccgatgcaat tccgagcacg ctggccttgc cgcgcgccgc ccattcgatg gccatccagg
     4201 tgagcgcggc ggtggccgcg gcgcaatgcg tgttggcgaa cgcgagacca gctcggccat
     4261 cggcagacag cgccgaaccc gcgttgaatc cgaaccagcc gacccacagc agtgcggcgc
     4321 cggtgatggt gagcgcaagg ttgtgaggga agatgggatc ggtgccgtaa cctttgcgct
     4381 cgccgagcat gatcacgcag gcaagaccgg tgacaccggc gttgatg
//
LOCUS       DASQJS010000164         8919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1031460, whole genome shotgun sequence.
ACCESSION   DASQJS010000164 DASQJS010000000
VERSION     DASQJS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031460"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VEW08_06880"
     CDS             complement(<1..952)
                     /locus_tag="VEW08_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYJ40494.1"
                     /translation="MNLSRSSIKTSFDVVIVGGGHNGLTCACYLAKAGLSVGVFERRS
                     ILGGAAVTEEFHPGFRNSTASYTVSLLNPKVIHDLRLVEHGLTVLERPMQNFLPQPDG
                     RALTAGPDTARTTASVRSFSARDAQRLPAYYKMLEAAVTLLRSQLLETPPANPRRLRD
                     LWGALQMGRRFRQLDASVQRDIHELFTRSAGDLLDHWFESDAVKALYGFDAVVGSYAS
                     PYTPGSAYVLLHHVFGEVNGKPGVWGHAVGGMGAITQAMANEARRLGVTLETDAPVKR
                     IVVERGRATGIELEDGRVILGNSVAANLGPKPLFLKLLADA"
     gene            998..1630
                     /gene="lexA"
                     /locus_tag="VEW08_06885"
     CDS             998..1630
                     /gene="lexA"
                     /locus_tag="VEW08_06885"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HYJ40495.1"
                     /translation="MSDLTPRQTEILRLIQRAINETGMPPTRAEIAGELGFKSPNAAE
                     EHLRALARKGVIALVPGASRGIKLMDTMREQMGLPLIGRVAAGRPILAEENIESRLDI
                     DPAIFQPKPHYLLKVVGMSMKDAGILDGDLVAVHRTPEVRNRQIIVARLENEVTVKRY
                     KQDGAVVWLLPENADFEPIRVNIKEDPLIIEGVVVGVLRRGDPLIKKGLS"
     gene            1654..2145
                     /locus_tag="VEW08_06890"
     CDS             1654..2145
                     /locus_tag="VEW08_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ40496.1"
                     /translation="MYAAASAALRVARERLEIGARAPGNALEIHMLLGLRSAFCYVDD
                     IAAAKLWYTKVLGFPPYFDQPFYVGFNVGGFELGLSPDGGPDGGPDGGRGKGPGNSPR
                     AYGAYWGVADVKSVYARLLELGAAPRGEIQDVGEGILIADVLDPWGNVFGIIENPNFK
                     LSP"
     gene            2154..2552
                     /locus_tag="VEW08_06895"
     CDS             2154..2552
                     /locus_tag="VEW08_06895"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HYJ40497.1"
                     /translation="MKRASGSFEVSMQSLANSEVSADPMLGRFLLTKKFTGDLDASAR
                     GHMLSAGTGVKGSAGYVAIDQVTGSLDGRQGSFVLQHSGSMKRGAPTLSVMVVPDSGT
                     GELQGLTGTLSINIVEGKHFYDFLYSFPAS"
     gene            2569..3210
                     /gene="imuA"
                     /locus_tag="VEW08_06900"
     CDS             2569..3210
                     /gene="imuA"
                     /locus_tag="VEW08_06900"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HYJ40498.1"
                     /translation="MLETHEKQLEELLAHPSVWRGRSRAAVETFPTGFAALDAGLPGG
                     GWPRYGLVEILTPQPGVGELYLLLPALAALSHLLPARWCTWVSPPHEPFAPALAAHGV
                     ALDRMLIVRTHLPLWAHEQALRSGACEVALAWLPRASPRAVRRLQLAAEQGRALGVLY
                     RSQKFASEASPAMLRVLLEPRVAAGRQGARLSLLKSRGGSREPIDLTWAPAAA"
     gene            3223..4842
                     /locus_tag="VEW08_06905"
     CDS             3223..4842
                     /locus_tag="VEW08_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HYJ40499.1"
                     /translation="MRHAKTLFEPGDVSAAPSVLRVRPAPPPPTGPGSPGQAALALDP
                     RELWIAAHVPHLSLLSLPAGSSLAPLVVLDEARNPRIVDADPRAQAAGIRIGMTLGAA
                     LAAAPHIDARPRDVARELALMQRLAGIAAAFTPQVSIEAPDGLLLEIKPSIRLFGGLK
                     ELCRQLRTACLADAAFASSGAQPHFTLAPTALAALAAARAGARCFITDPRQLPARLKL
                     LPAGVLRWPGDANARLLAMGVRTLGDLLRLPRAGFAKRFGPALLADLDRLQGRSADPR
                     RRITRRERYAGRVDLDHEIEDHDRILDALRPLLDELEQFLRKRQRGITALQCRFHHYR
                     APPTTCALRLAAPEADATRLQSLLRERLASLRLPEPVRRCELRGGALTERPLASKPLW
                     SAGERGHAPANEMPALIEHLRARLGADAVYGIGRVPEHRPENAWRVAEPALAAVVSKD
                     AAGDPSVTPFHRPLWLLHAPQELDSQRGRPRRGGALELLAGPELIESGWWDGADARRD
                     YYVAGDARGSRLWIYRECAGARKWYLHGIFG"
     gene            4839..8075
                     /locus_tag="VEW08_06910"
     CDS             4839..8075
                     /locus_tag="VEW08_06910"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HYJ40500.1"
                     /translation="MSAQTPVNTAPAYAELHCLSNFSFLRGASHAEELVVRANELGYT
                     ALAITDECSLAGVVRAHSAVKKVGGVELPIKPSIKPSIKLIIGAEFALTCGLRVVVIA
                     RNRAGYGRLSRLITRGRRAAVKGSYALTREDVEEFIGDAALPREGDATLPREGAAAPP
                     RDHEAVAEGTLALWLPALDADIAGQNTDEGEWMARTFAGRAWIAVELTRDGRDRERLA
                     QCRALGAALNLPLTAAGDVHMHVRERRRLQDALTAVRHGVPVAEAGTRLHPNGERHLR
                     ERARLARLYPPELLAETVKIAALANFSLDELRYEYPHELVPAGETAASHLRKLTEAGA
                     RKRWPAGIPAKMRAQIDYELALINELTYESYFLTVHDIVNEARRLNILCQGRGSAANS
                     VVCYSLGVTEVDPSRGELLMERFISRERNEPPDIDIDFEHERREEVIQYIYRKYGRDR
                     TALTATVICYRPRSALRDMAKVMGLDTVQAERLAGAMQWWDTAVDEERVREAGFEPGN
                     RQVRLLLELAGELQGFPRHLSQHVGGFVISQGLLEELVPIENAAMQDRTVIEWDKDDL
                     DELGLLKVDVLGLGMLSALRRGLALVSDFRRKPFTLADIPKEDPRVYDMISRADTIGV
                     FQVESRAQMSMLPRLQPKEFYDLVIEVAIVRPGPIQGGMVHPYLRRRQDLEPVTYPSD
                     AVKGVLSRTLGVPIFQEQVMQLAIVAAGFTPGEADQLRRSMAAWKRRGGLGHFERRLI
                     DGMRARGYDESFARQIFSQIQGFGEYGFPESHAASFALLVYSSAWLKCHEPAAFTCAL
                     INSQPMGFYAPAQLVRDARDHGVEVRPISVCSSHWDCTLERRADDEPALRLGLRLVKG
                     LNEEAALRIVAARVQAEVASVQDLTLRANLARRELEALADAGALRELSGNRHLTFWQV
                     AGSEQELPLAPVPRAAEGTPLLAPPTEGQNIAADYRTQGLTLGRHPLALLRADLSAAH
                     IVTSADLRDLPHGRVVRVAGIVTARQRPQSAGGVMFITLEDETGCVNLIVWERVWSRA
                     RRIASGARLLEAAGMLQKEGLVTHVVAQKLFDRTSMLGVIVTQSRDFR"
     gene            complement(8122..8640)
                     /locus_tag="VEW08_06915"
     CDS             complement(8122..8640)
                     /locus_tag="VEW08_06915"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ40501.1"
                     /translation="MTQTHTRKPIVLGPGEGRSYPMGRIDAVFKADGAETGARFSISE
                     WWLEPHTAGPGPHSHPEDDIFYVIGGTMSVLVGNEWTQAAPGSFVLIPGGVTHDFENR
                     GEVRAGVLNFFAPGTFEEHMPEIARWWRDNPPKELVPKELGSGTDSPLRGQMGSGLLS
                     ASRSSDDNMNEV"
     gene            complement(8708..>8919)
                     /locus_tag="VEW08_06920"
     CDS             complement(8708..>8919)
                     /locus_tag="VEW08_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40502.1"
                     /translation="TSEVDFDRLAKDLDAAKKRGQKLGDPAWRRLERRFEDKHTAELT
                     SDFDDYNIDDDSTAVRKMNNSKNAS"
BASE COUNT         1447 a         2887 c         3017 g         1568 t
ORIGIN      
        1 cggcatcggc cagcagtttg aggaagagtg gtttgggccc gagattcgcc gcgaccgagt
       61 tgccgaggat cacgcgtcca tcctcgagct cgatgccggt ggcgcggccg cgttcgacga
      121 cgatgcgttt cacgggtgca tcggtttcga gtgtgacgcc gagccgtcgc gcctcgttgg
      181 ccatggcttg cgttatcgct cccattccac cgaccgcgtg tccccagaca ccgggcttgc
      241 cattcacttc accgaacacg tggtggagaa gtacgtaggc gctgccgggc gtgtagggac
      301 tcgcgtagct gccaactacc gcatcgaagc catacagcgc cttgaccgcg tcgctctcga
      361 accagtgatc taacaagtcg ccggcgctgc gcgtgaacag ttcgtggatg tcgcgctgca
      421 cactggcgtc gagttggcgg aagcggcgtc ccatctgcag cgcgccccac aggtcgcgca
      481 gtcggcgcgg attcgcgggc ggcgtttcca gcagttgcga tcgcagcagg gtcactgccg
      541 cttcgagcat cttgtagtag gcaggcaggc gttgcgcatc gcgtgccgag aagctgcgca
      601 cggaggccgt ggtgcgggcg gtgtccgggc cggcggtgag cgcgcggcca tcgggctgcg
      661 gcagaaagtt ctgcatgggc cgctcgagga ccgtgaggcc gtgttcgacc aggcgcagat
      721 cgtggatcac cttggggttc agcaggctga ccgtgtagct cgccgtggag ttgcggaagc
      781 cggggtggaa ttcctcggtg acggcggcgc ctccaaggat ggaacggcgc tcgaagacgc
      841 cgacggagag gcccgccttg gcgagatagc aggcgcaggt aaggccgttg tggccgcctc
      901 cgacgatgac cacatcgaag ctggttttga tgcttgacct ggataaattc acagtgtaca
      961 aatcctcagc cctgattctt ctttaaagga atccgccatg tccgacctca ctccacgcca
     1021 aaccgaaatc ctgcgtctca tccagcgcgc catcaacgaa accggcatgc cgcccacccg
     1081 cgcggaaatc gccggcgaac ttggtttcaa atccccgaac gccgccgaag aacacctgcg
     1141 ggcgctcgca cgcaagggcg tcatcgcgct cgtgccgggc gcttcgcgtg gcatcaagct
     1201 catggacacc atgcgcgaac aaatgggtct gccgctcatc ggccgtgtcg ccgcgggccg
     1261 ccccattctg gccgaggaga atatcgaatc ccggctcgac atcgaccccg ccatctttca
     1321 acccaagccg cattacctgc tcaaggtcgt cggcatgtcg atgaaagacg ccggcattct
     1381 cgatggggac ctggtggccg tgcatcgtac gcctgaagtc cgcaaccggc agatcatcgt
     1441 cgcgcgcctc gaaaacgaag tcaccgtgaa gcgttacaag caagacggcg cggtggtctg
     1501 gctgctgccg gagaacgcgg acttcgaacc catccgcgtc aacatcaagg aagacccgct
     1561 catcatcgaa ggtgtggtgg tgggcgttct gcggcggggc gatcctttga taaagaaagg
     1621 gctgtcgtga tccgtcgtcg tcttcgaaag tagatgtacg ccgccgcttc ggcggcgtta
     1681 agagtggcgc gtgagcgcct ggagataggg gcgcgagccc ctgggaatgc cctggaaata
     1741 cacatgttgt taggactgcg ttccgccttt tgttatgtcg atgacattgc cgcggccaag
     1801 ctctggtaca cgaaagtgct gggctttccg ccttacttcg accagccgtt ctacgtgggt
     1861 ttcaatgtcg gcggtttcga gctcgggctc tcgcctgatg gagggcctga tggagggcct
     1921 gatggagggc gtggcaaagg gccgggtaac tctccgcggg cctacggtgc ctactggggc
     1981 gttgcggacg tcaaatcggt gtacgcccgg ctgttagagc ttggggctgc gcctcgcggc
     2041 gagatccagg acgtgggcga aggcatcctc atcgccgacg tgctcgaccc ctggggcaat
     2101 gtgttcggga tcatcgagaa cccgaacttc aagttatctc catagaagtg gatatgaaac
     2161 gcgcgtccgg aagttttgaa gtcagcatgc agtcgctggc gaatagtgaa gtatcggcag
     2221 atcccatgct gggccgcttc ctgctcacca agaaattcac cggagatctc gatgcctcgg
     2281 cgcgtggaca catgttgtcc gccggcactg gcgtcaaagg ctccgcaggt tacgtggcca
     2341 tcgatcaggt cacgggctcc ctcgacgggc gccagggcag tttcgttctg cagcactcgg
     2401 gaagcatgaa gcgcggtgcg ccaactctct cggtcatggt ggtgccagat tcgggaaccg
     2461 gtgagctcca ggggctcacg ggcacgctca gcatcaacat cgtggaaggc aaacacttct
     2521 acgacttcct ttactcgttc cctgcgagct gatgtcgcca gcacttccat gctcgaaacc
     2581 cacgaaaagc aattggaaga gttgctcgcc cacccgtcgg tgtggcgcgg gcgcagccgc
     2641 gccgcggtgg aaactttccc caccggtttc gctgcgctcg atgcggggct gcccggcggc
     2701 ggctggccgc gctacggcct cgtagaaatc ctcacgccgc agcccggcgt gggcgagctc
     2761 tatctactgc tgcccgcgct cgcggcgctg tcgcatcttc tgccggcgcg ctggtgcacc
     2821 tgggtgtcgc cgccgcacga gcccttcgca ccggcgctcg cggcgcatgg cgtggcgctc
     2881 gatcgcatgc tcatcgtgcg cacgcatctg ccgctgtggg cgcatgagca ggcgctgcgt
     2941 tcgggtgcgt gtgaggtggc gctggcctgg ctgccgcgcg ccagcccgcg cgctgtgcgc
     3001 cgcctgcagc tggcggccga gcagggccgg gcgcttggcg tgctttatcg cagccagaaa
     3061 ttcgcgagcg aagcttcacc ggccatgttg cgggtgttgc tcgagcccag ggtcgcggcg
     3121 ggcaggcaag gcgcgcggct gagtctgctc aagagccgcg gtggcagccg cgagcccatc
     3181 gacctcacgt gggctcccgc cgcggcatga gtgggcgcag cgatgcgaca cgcaaaaacg
     3241 ctttttgaac cgggggatgt gagtgcggcg ccgtccgttt tacgggtgcg gccggcgccg
     3301 cctccgccca cggggccggg gtctcccggc caggcggcgc tggcactcga tccacgcgag
     3361 ttatggatcg ccgcgcatgt gccgcacttg tcgctgctct cgttgcctgc gggttcgtcg
     3421 ctcgcgccgc tcgtggtgct cgatgaggcg cgcaatccgc gcatcgtcga cgcggacccc
     3481 cgggcgcagg cggcggggat ccgaatcggc atgacgctgg gtgcggcgct tgcggccgcg
     3541 cctcacatcg acgcccggcc gcgtgacgtg gcgcgcgaac tggcgctcat gcagcggctg
     3601 gccggcatcg ctgccgcgtt cacaccgcag gtgtccatcg aagctccgga cggtctgctg
     3661 ctcgaaatca agccgagcat ccgcctgttc ggaggcctga aagagctgtg ccgccagctg
     3721 cgcacggcct gcctcgcgga tgccgcgttt gcgagcagcg gcgcgcagcc gcatttcacg
     3781 ctggcaccca cggcgctggc ggcgttggcg gccgcgcgtg ccggcgcacg ctgtttcatc
     3841 accgatccgc gtcagttgcc ggcgcggctc aaactactcc cggccggggt cctgcgctgg
     3901 cccggagacg ccaatgcgcg gttgctcgcc atgggcgtgc gtacgctcgg cgatctgctg
     3961 cgcctgccgc gcgccgggtt tgccaaacgt ttcggcccgg cacttctggc agatctggat
     4021 cgcctgcagg ggcggagcgc cgatccgcgc cggcggatca cgcggcgcga gcgttacgcg
     4081 gggcgcgtgg atctcgatca tgaaatcgaa gatcacgatc gcatcctcga tgcgctgcgg
     4141 cccttgctgg acgaactcga gcaattcctg cgcaaacggc agcgtggcat caccgcgttg
     4201 caatgccgtt tccatcacta ccgcgcgcca ccgacgacat gtgcgctgcg cctggcggcg
     4261 cccgaggcgg atgccacgcg gcttcagtcg ctgctgcgtg aacgcctggc aagtctcagg
     4321 ttgcccgagc ccgtgcggcg ttgcgaactg cgtggcggcg cgctcacgga gcggccgctc
     4381 gcgagcaaac cgctgtggtc cgccggtgag cgtggccacg cgccggcgaa cgagatgccg
     4441 gcgctcatcg agcatctgcg cgcgcgcctc ggcgccgatg cggtgtatgg catcggccgc
     4501 gtgccggaac accggcccga gaacgcctgg cgggtggcgg agcccgcgct cgccgcggtc
     4561 gttagtaagg atgccgccgg cgatccgtcc gtgacgccgt ttcatcgtcc cctgtggctg
     4621 ctgcacgcgc cgcaggaact cgattcgcag cgcgggcggc cgcgccgtgg cggagcgctc
     4681 gaattgctgg ccggccccga gctcatcgag agcggctggt gggatggcgc ggatgcccgg
     4741 cgcgactact acgtggcggg tgatgcgcgc ggctcccgtt tgtggattta ccgcgaatgc
     4801 gccggcgcca ggaaatggta cctgcacgga attttcggat gagcgcccag actccagtca
     4861 atacggctcc tgcctacgcc gagctgcatt gcctcagcaa cttcagtttc ctgcggggcg
     4921 cctcgcatgc ggaagagctg gtcgtgcggg cgaacgagct cggatacacc gcgcttgcca
     4981 tcaccgacga atgttcgctc gccggcgtgg tgcgcgcgca ttcggcggtc aagaaggtcg
     5041 gtggcgtcga actacccata aagccatcca taaaaccctc cataaaattg atcataggcg
     5101 cggagttcgc gctcacctgc ggactgcgcg tggtggtcat cgcgcgtaac cgcgccggat
     5161 acgggcggct gtcacggctg atcacgcgtg gacggcgtgc ggccgtcaag ggcagctacg
     5221 cgctcacgcg tgaagacgtc gaggaattca ttggtgatgc cgcgctgccg cgcgagggtg
     5281 atgccacgct gccgcgcgag ggcgccgccg ccccgccacg cgatcacgaa gccgtggcgg
     5341 aaggcacgct cgcgttgtgg ctgccggcgc tcgacgccga cattgcaggc cagaatactg
     5401 acgagggcga atggatggcc cgcactttcg ccggtcgcgc ctggatcgcg gtggagctca
     5461 cgcgtgatgg ccgcgatcgc gaacggctgg cgcagtgccg cgcgctcggc gccgccctca
     5521 acctgcctct gaccgcggcc ggtgacgtgc acatgcacgt gcgcgagcgg cgccgcctgc
     5581 aggatgcgtt gaccgcggtc cgtcacggag tcccggtggc cgaggcgggc acccggctgc
     5641 atcccaatgg cgagcgccac ctgcgcgaac gagcgcggct ggcccggctc tatccacccg
     5701 aactgctggc cgaaaccgtg aagatcgccg cgctcgcgaa cttctcgctc gatgagctgc
     5761 gctacgagta cccgcatgaa ctggtgccgg ccggcgagac cgcggcctcg cacctgcgca
     5821 aactcacgga agccggcgcg cgcaagcgct ggccggcggg cattccggcg aagatgcgcg
     5881 ctcagatcga ctacgagctc gcgctcatca acgagctcac ctacgagtcc tacttcctca
     5941 cggtgcacga catcgtgaat gaggcccgcc gcctcaacat cctctgccag gggcggggtt
     6001 cggccgccaa ctcggtggtg tgttattcgc tgggcgtcac cgaggtggac ccatcgcgcg
     6061 gtgagctgct catggagcgt ttcatttcac gcgagcgcaa cgagccgccc gacatcgaca
     6121 tcgacttcga acacgagcgg cgcgaagagg tcattcaata catctatcgc aagtacggcc
     6181 gcgatcgcac cgcgctcacg gccacggtca tctgctatcg gccgcgcagc gccttgcgcg
     6241 acatggccaa ggtgatgggg ctcgacaccg tgcaggccga acgtctcgcg ggcgccatgc
     6301 aatggtggga caccgcggtc gacgaagagc gggtgcgcga ggccggtttc gagcccggca
     6361 accgccaggt caggttgctg ctcgagctgg ccggcgagct gcagggtttt ccgcggcacc
     6421 tgtcccagca cgtcggcgga ttcgtgatct cgcaagggct gttggaagag ctcgtcccca
     6481 tcgaaaacgc cgccatgcag gatcgcacgg tcatcgaatg ggacaaggac gatctcgatg
     6541 agctcggcct gctgaaagtg gacgtgctcg gcctcggcat gttgtcggcg ctgcgccgcg
     6601 ggctcgcgct ggtgtcggac ttccggcgca aacccttcac gctcgccgac attccgaagg
     6661 aagacccgcg cgtctacgac atgatttcgc gcgccgacac catcggcgtg ttccaggtgg
     6721 agtcgcgcgc gcagatgtcc atgttgccgc gcctgcagcc aaaagagttc tacgacctgg
     6781 tcatcgaagt ggccatcgtg cgtcccgggc ccatccaggg cggcatggtg cacccctatc
     6841 tacgccggcg gcaggatctc gagccggtca cgtatccgag cgacgccgtg aagggcgtgc
     6901 tgtcgcgcac cctgggggtg cctatcttcc aggaacaggt gatgcagctc gccatcgtgg
     6961 ccgccggctt cacgccgggc gaggcggatc agctgcgccg gagcatggcg gcctggaagc
     7021 ggcgcggcgg tctcgggcat ttcgagcggc ggctcatcga cggcatgcgc gcgcgcggtt
     7081 atgacgaaag cttcgcgcgg cagatcttca gccagataca gggtttcggc gaatacggtt
     7141 ttcccgagtc gcacgccgcg agtttcgcgc tgctggtgta cagctccgcg tggctcaagt
     7201 gtcatgaacc cgcggctttc acctgcgcgc tcatcaacag ccagcccatg ggcttctatg
     7261 cgccggcgca gctggtgcgt gacgcgcgcg accacggcgt tgaagtgcgg ccgatcagcg
     7321 tctgcagcag tcattgggat tgcacgctcg agcgtcgcgc agatgacgag cccgcattgc
     7381 gactggggct gcggctggtg aaaggcttga atgaagaggc cgccctgcgc atcgtcgcgg
     7441 cgcgtgtcca ggcggaagtc gcgagtgtgc aggacctcac gctgcgcgcg aatcttgcgc
     7501 gccgcgaact cgaagcgctg gcggacgcgg gcgcgttgcg cgagttgtcg ggaaatcgtc
     7561 atctcacttt ctggcaggtg gcgggcagcg agcaggaact gccgctcgcg cccgtgccgc
     7621 gcgcggccga aggcacaccc ttgctcgccc cgcccaccga agggcagaac atcgccgcgg
     7681 actaccgcac ccagggcctc actctggggc gccatccgct ggccctgctg cgtgccgacc
     7741 tctcggctgc ccacatcgtc acctcagccg atctgcgcga cctgccgcac gggcgcgtgg
     7801 tccgtgtggc cggtatcgtc acggcgcgcc agcgcccgca gagtgcgggt ggcgtcatgt
     7861 tcatcacgct cgaggacgaa accggctgcg tgaacctcat cgtctgggaa cgcgtgtggt
     7921 cgagggcgcg gcgcatcgcc agtggagcgc ggctgctcga agcggccggc atgctgcaga
     7981 aagaggggct ggtcacccac gtggtggcgc agaagctgtt cgaccgaacg agcatgctcg
     8041 gggtgatcgt gacgcagtcg cgtgacttcc gctgaaatgc caaaggaatc ggggtcaggc
     8101 accgattcct agcaaatggg gtcagacctc attcatgttg tcgtcgctcg atcgcgatgc
     8161 gctcaagaga cctgacccca tttgcccgcg aagcggggaa tcggtgcctg accccaattc
     8221 ctttggcacc aattccttgg gcgggttgtc acgccaccag cgcgcgatct ccggcatgtg
     8281 ctcctcgaag gttcccggcg cgaagaagtt gagcaccccg gcgcgaacct cgccgcgatt
     8341 ctcgaaatcg tgcgtgaccc cgccaggaat gagcacgaat gagccgggcg cggcctgggt
     8401 ccattcattc cccacgagca cgctcatcgt gcccccgatc acatagaaga tgtcgtcctc
     8461 gggatgcgaa tgcgggccgg ggcccgcggt gtgcggctcg agccaccatt cggaaatgga
     8521 gaaccgcgcg cccgtttcgg cgccatcggc cttgaacacg gcgtcgatgc ggcccatggg
     8581 ataggagcgg ccctcaccgg ggccaagcac tatgggttta cgtgtgtgag tttgtgtcat
     8641 ggcgcggatc gtaaacgccc ggccagcctg caaacgtcaa gaagtgtcgt cgacccgaag
     8701 gagtgcgtca gctcgcgttc ttcgagttgt tcatcttgcg cacggccgtg ctgtcgtcgt
     8761 cgatgttgta gtcgtcgaag tcactggtga gttcggcggt gtgcttgtct tcgaaacgcc
     8821 gctcgagacg gcgccaggca gggtcgccca gcttctggcc gcgcttcttg gctgcgtcca
     8881 gatctttcgc aagacgatcg aagtcgactt cgctggtag
//
LOCUS       DASQJS010000165         8015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4487620, whole genome shotgun sequence.
ACCESSION   DASQJS010000165 DASQJS010000000
VERSION     DASQJS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4487620"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..523
                     /gene="mreD"
                     /locus_tag="VEW08_06925"
     CDS             44..523
                     /gene="mreD"
                     /locus_tag="VEW08_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HYJ40503.1"
                     /translation="MNFARREPRWAVTFTVLLALIAQVVPLPDWLAGIRPSFIALVII
                     YWSIYAPHAGGIFAPWLAGLALDLFKGEVLGQNALAIALIAYIAMSLHQRLRNQTLVQ
                     QSLFVFAMLFLNETVVWGIEGWSGHSAASPWRWIQPMIGAMLWPFIALLVGRTHTRS"
     gene            542..2506
                     /gene="mrdA"
                     /locus_tag="VEW08_06930"
     CDS             542..2506
                     /gene="mrdA"
                     /locus_tag="VEW08_06930"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYJ40504.1"
                     /translation="MGTVRIKDHWGEQRLFESRALAAAFIIAALTIVLVGRLVVLQVM
                     RYDYYAELSQGNRVRVEPLPAQRGLILDRNGSVLAENRPAFQLELVRERVPDVEATLK
                     RLIEIGVLPLDDLDETRKLVKSRRSFESVPIRLRLTDEEIARFAVHRFEFPGVDIATR
                     LTRFYPHGEHAVHALGYVAAISESDVKKLEKEKLLSKYAGTSLIGKLGIEASFEETLH
                     GVDGSRQILVNAAGRSVQRHGTVKSDLKEIDPVPGRDLITGIDLPTQLVAEQGLINRR
                     GAVVAIDPNTGDVIALVSTPGFDPNPFGRGLTTAEYGALRDNIDIPLLNRAIRGEYPP
                     GSTVKPAIALGGLTLGVTTPQHTKSCPGYFMLPGSRHRYRDWRPSGHGVVAMETAIAQ
                     SCDVYFYELSSMLGVEKLAGWLAHFGLGAATNIDIDGERRGILPTPAWKKARFKNPAD
                     QIWFPGETVSFGIGQGYLIVTPIQAAHMASILASRGKSFQPRLVTAYREQGTGKIEKI
                     APKLLETVEASPENWKIITDGMVKVMSGNGGTGRRSQVGAEYQIAGKTGTAQVFTVKQ
                     NESYKEMEARGLNERLLDHGWFIAFAPADAPKLAVAVIIENGKHGTTAAPIARRVLDQ
                     YLLGRTTTPEIPVYVSAQGPAAAADAPGDE"
     gene            2506..3645
                     /gene="rodA"
                     /locus_tag="VEW08_06935"
     CDS             2506..3645
                     /gene="rodA"
                     /locus_tag="VEW08_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HYJ40505.1"
                     /translation="MYEPIEIGSRSRRTFTAAGRILGALKLDGPLLVGLGLITLYGLV
                     VLYSASGQNWNRVIDGAIRAALGGVAMCALAQVKPAFLRRLSLPLWVLGVILLIVVDI
                     TGHIGKGAQRWLDLGFIRFQPSEIMKLAVPMTCAWWLHERPLPPNIPSLAALAFIILL
                     PTGLTIVQPDLGTGGLILIGGTMVVILAGLQFRVIFGLAAAGAAAGWFAWHYLLYDYQ
                     KQRVFTLLDPESDKLGAGYHIIQSQIAIGSGGVFGKGLLNGSQGQLEFLPERTTDFIY
                     AMVGEELGLLGATILLLLYVFVVGRALYLAVETQDTFARLLAGSIALTFFVYVFINAG
                     MVSGLLPVVGVPLPLVSYGGTSMVTLLASFGILMSLYSHRKLVAS"
     gene            3645..4715
                     /gene="mltB"
                     /locus_tag="VEW08_06940"
     CDS             3645..4715
                     /gene="mltB"
                     /locus_tag="VEW08_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HYJ40506.1"
                     /translation="MRNAPALTAILLFSSGSALADGSFVGRTRFDLDRTEIQQFVEAT
                     AATNKMEPLDVYRLLAKAEPQPKIIELITRPAEKVSPWWEYRKNFLTEQRVTEGMQFM
                     REHRARLEKARKETGVAPEYIVAIIGVETYYGRITGKYRVLDALATLAFDYPQRGEYF
                     RGELAQFIALSREEAVDPLTALGSYAGAMGASQFMPSSYRAYAVDGGNDKQRDLFADW
                     DDIIASVANYFRKHGWVTNGPVLAEASLQPDAPVTADPGNLSLNETVAGLKSKGVDFD
                     TAQAETTPVLLIPAETELGPSYRVGFKNFEVITRYNRSVRYAMAVHDLATTIADRVAA
                     AGPAPGGETKLAAQPGSAAATR"
     gene            4715..5611
                     /locus_tag="VEW08_06945"
     CDS             4715..5611
                     /locus_tag="VEW08_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HYJ40507.1"
                     /translation="MGNVPRAAIALTLTAFLLSACSLARRQPDKPLPDKQSTSIPSGS
                     AVPQPPPDVFSIPDAVPRAEPRGTRGNPPFYEVFGKRYYVLASSEGFVERGTASWYGP
                     GFHAASTSLGERYDMYAMTAAHKTLPIPAYAEVTNLKNGRKVVVRINDRGPFVGDRII
                     DLSYTAAAKLDMLLAGTAPVEVRVITARGAGSALPQPGVPAPVAPALPPVAVVNAGIK
                     PGAPAMFIQAGVFSDHENARRRVEVLLASGIELASLDEIPRNERALHRVRVGPFASVE
                     EFDFNMKRLRELGINDARLLTD"
     gene            5707..6843
                     /locus_tag="VEW08_06950"
     CDS             5707..6843
                     /locus_tag="VEW08_06950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HYJ40508.1"
                     /translation="MPSSRLISYLACIFLANSALGAVPIPKPPAIDARAYVLIDFQSG
                     RVLAAEKADVRNEPASITKVMTGYGVFRAVREKRLSLTDSVTISEHAWKAEGSRTFAQ
                     VGTQIPVDTLIKGMIVQSGNDATIALAEKLGGTEDGFVQMMNAYAAELGLKNTHFDNS
                     WGGPGPTHYMSAMDIALLARILIRDYPEEYKLYSLREFEWNGIKQQNRNGLLARDPSV
                     DGIKTGHTESAGYCLASSATRNGMRLISVVLGTQSFKAREDASAALLNYGYTFYETVQ
                     VKKAGDVVLKPEVYKGEEDRVTLVPARDVFVTIGRGSAAQLKTTATVKTPVIAPIIAG
                     QTLGELTVSDGKDIIARVPLVAQAAVPEGGWWTRMVDGISLWME"
     gene            6854..7732
                     /locus_tag="VEW08_06955"
     CDS             6854..7732
                     /locus_tag="VEW08_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HYJ40509.1"
                     /translation="MGEPLPICFLNDDFLPLREARISPLDRGFLYSDGAYEVTPVYAG
                     RPFRFEQHHARLTRSLREIRMEDPLSREAWRDLYRELISRNGAESSDAYIYVQVTRGA
                     EHGRNHAPLPDVPRTVFAFVAPWPTGRPGAFENGMPAVTADDTRWARCDIKSVSLLAN
                     VLLRQLAVDENASETILIREGFLTDASASSVHVVQGGVLCTPPNSWKLLPGTTRGVIE
                     EIAERAGVIWRSVAISEPDLRSASEILLGAATREIQPVTTLDGKPVGDGKPGPVWRRL
                     YDAYQAYKHEIAGTPW"
     gene            7726..8004
                     /locus_tag="VEW08_06960"
     CDS             7726..8004
                     /locus_tag="VEW08_06960"
                     /inference="COORDINATES: protein motif:HMM:NF016262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HYJ40510.1"
                     /translation="MVDEIPPSEELLKFPTSYPIKVVGRRSEHMRANIDAIVRAHVPD
                     LTADQIKERESTQQHFLAITYTINAQSKEQVVALAHALQASKDVIMLI"
BASE COUNT         1455 a         2701 c         2478 g         1381 t
ORIGIN      
        1 cgcccgaggc tctggcgatc gaaccgccgg gggttaaaac accatgaact tcgctcgccg
       61 cgaaccgcgc tgggcggtca cgttcacggt gctgctggcg ctcatcgcgc aggtggtgcc
      121 actgccggac tggctggcgg gtatccggcc gtcgttcatt gcgctggtca tcatctactg
      181 gagcatctac gcgccccacg ccggcggcat cttcgcgcca tggctcgcgg gtctcgcgct
      241 cgacctgttc aagggcgagg tgctggggca aaacgcgctc gccatcgcgc tgatcgccta
      301 catcgccatg agcctccatc agcggctgcg caaccagacc ctggtgcagc agtcactgtt
      361 cgtgttcgcc atgttgttcc tcaacgagac ggtcgtctgg ggcatcgagg gctggagcgg
      421 ccattcggcg gcgtccccgt ggcgctggat acagccgatg atcggcgcca tgttgtggcc
      481 gttcatcgcg ttgctggtcg gccgcacgca cacgcgtagt tagaggcgcg gcagaaaacc
      541 catgggcacc gttcgcatca aggatcactg gggcgagcag cggctgttcg agagccgcgc
      601 gctggccgcg gctttcatca tcgccgcgct caccatcgtg ctcgttggcc gcctggtcgt
      661 cctgcaggtc atgcgctacg actactacgc cgagctctcg caggggaatc gcgtgcgcgt
      721 cgaaccgctg cccgcgcagc gcgggctcat tctcgaccgc aatggatcgg tgctggcgga
      781 gaaccgtccc gccttccagc tcgaactggt gcgcgagcgc gtgccggacg tcgaagccac
      841 cttgaagcgg ctcatcgaga tcggcgtgtt gcccctcgac gacctcgatg aaacccgcaa
      901 gctcgtgaaa tcacgccgca gcttcgaaag cgtgccgatc cgcctgcgcc tcacggacga
      961 ggaaatcgcc cgcttcgccg tgcaccgttt cgaatttccc ggcgtggaca tcgccacgcg
     1021 actcacgcgt ttctatccgc acggcgaaca cgccgtgcac gcgctcggct acgtcgcggc
     1081 gatttccgaa agcgacgtga agaaactcga aaaggaaaaa ctgctttcca agtacgccgg
     1141 cacctcgctc atcggcaagc tcggcatcga ggcgtccttc gaggaaacgc tgcatggcgt
     1201 ggacggttcg cgccagatcc tggtcaacgc cgccgggcgt tcggtgcaac gccatggaac
     1261 ggtcaagtcg gatctcaagg agatcgatcc ggtgccgggt cgcgatctca tcacgggcat
     1321 cgacttgccc acgcagctcg tggccgaaca gggcctcatc aaccggcgcg gcgcggtcgt
     1381 ggccatcgat ccgaacaccg gtgatgtcat tgccctcgtg tccacgccgg gctttgaccc
     1441 gaatcctttc ggccgcggtc tcaccacggc cgaatatggc gcgctgcgcg acaacatcga
     1501 tataccgctg ctcaatcgcg cgatccgcgg cgaatatcca cccggctcga ccgtgaagcc
     1561 ggcgatcgcg ctcggcggcc tcacacttgg cgtcacgacg ccgcagcaca ccaaatcctg
     1621 ccccggttat ttcatgctgc ccggctcccg ccaccgctat cgcgactggc gcccgagtgg
     1681 tcacggcgtc gtcgcgatgg aaaccgcgat cgcgcagtcc tgcgacgtgt acttctacga
     1741 actctcctcg atgctgggcg tcgagaaact cgcgggctgg ctcgcgcatt tcggtctcgg
     1801 cgctgccacc aatatcgaca tcgacggcga gcgccgaggc atcctgccaa cgcccgcgtg
     1861 gaagaaggca cgcttcaaga atccggccga ccagatatgg ttccccggtg agaccgtgag
     1921 cttcggcatc ggccagggat atctcatcgt cacgccgatc caggccgcgc acatggcgtc
     1981 gatcctcgcg tcgcgcggca agagcttcca gccgcgcctc gtcaccgcct accgcgaaca
     2041 gggcacgggc aagatcgaga agatcgctcc caaactactc gagacggtcg aagcatcgcc
     2101 cgagaactgg aagatcatca ccgacgggat ggtcaaggtg atgagtggca atggcggcac
     2161 cggccggcgc agtcaggtgg gcgcggaata ccagatcgcc ggcaagacgg gcaccgcgca
     2221 ggtattcacg gtcaagcaga acgagagcta caaggaaatg gaggcgcggg ggctcaacga
     2281 gcgcctgctc gatcacggct ggttcatcgc gttcgcgccc gcggacgctc cgaagctagc
     2341 ggtggcggtg atcatcgaaa acggcaaaca cggcacgacg gccgcgccca tcgcgcgacg
     2401 cgtgctcgac caatacctgc tcggcaggac gaccactccc gagatcccgg tctacgtttc
     2461 agctcagggc ccggcggccg cggccgacgc gccaggagac gagtgatgta cgaacctatc
     2521 gaaatcggtt cgcgcagtcg ccgcacattc accgccgccg gccgcattct cggcgcgctg
     2581 aagctcgacg gcccgttgct cgtgggcctg ggactgatca cgctttatgg actggtcgtg
     2641 ctgtacagcg cctcgggcca gaactggaat cgcgtcatcg acggcgccat ccgtgccgcg
     2701 ctcggtggcg tcgccatgtg cgcactggcg caggtcaaac ctgccttcct gcgccggctt
     2761 tccttgccgc tatgggttct cggcgtgatc ctgctgatcg tggtcgatat caccggccac
     2821 atcggcaagg gcgcacagcg ctggctcgac ctgggattca tccgtttcca gccgtctgaa
     2881 atcatgaagc tggccgtgcc catgacctgc gcctggtggc tgcacgaacg gcccctgccg
     2941 ccaaacattc cctcgctggc ggcgctcgca ttcatcatcc tgcttccgac tggattgacc
     3001 atcgtacagc ccgatctcgg cactggtggc ctcatcctca tcggcggcac catggtggtg
     3061 attctggccg gcctccagtt ccgcgtgatc ttcgggcttg ctgccgcggg cgccgctgcc
     3121 ggctggttcg cctggcacta cctgctgtac gactaccaga agcagcgcgt cttcacgctg
     3181 ctcgatcccg aatccgacaa gctcggcgcc ggctaccaca tcatccagtc gcagatcgcc
     3241 atcggctcgg gcggcgtgtt cggcaagggc ctgctgaacg gctcgcaggg ccagctcgag
     3301 ttcctgccgg agcgcaccac cgacttcatc tacgccatgg tcggcgagga actcggtctg
     3361 cttggcgcga caatcctgtt gctgctctac gtgttcgttg taggacgagc cctgtatctg
     3421 gcggtggaga cgcaggacac attcgcgcga ttgctggccg gcagcatcgc cctgaccttc
     3481 ttcgtgtacg tgttcatcaa tgcgggcatg gtcagcggat tgttgccagt tgtcggtgtt
     3541 ccgctaccgt tggtcagtta cggcggaaca tcgatggtga ccctgctggc cagcttcggt
     3601 atcctgatgt ccctctactc gcatcggaag ctcgtcgcct cgtgatgcgc aatgcgcctg
     3661 ctctgacagc aatccttctt ttttcttcgg gttccgcact ggcggacggc tcgttcgtcg
     3721 gtcgcacccg tttcgacctc gatcgcaccg aaatccagca attcgtcgaa gcaaccgccg
     3781 cgacgaacaa gatggaaccg ctcgacgtct accgtttgct cgccaaggcg gagccccaac
     3841 ccaagatcat cgagctcatc accaggcccg ccgagaaagt gtcgccctgg tgggagtacc
     3901 gcaagaactt cctgaccgag cagcgcgtga ccgaaggcat gcagttcatg cgcgagcatc
     3961 gtgcgcgcct cgagaaagcc cgcaaggaaa ccggcgtggc gccggaatac atcgtggcca
     4021 tcatcggcgt cgaaacctac tacggtcgca tcaccggcaa ataccgcgtg ctcgatgcac
     4081 tggccacgct cgcgttcgac tatccacagc gtggcgaata tttccgcggc gagctcgcgc
     4141 agttcattgc gctgtcgcgt gaagaggccg tggatccgct gaccgcgctc ggctcctatg
     4201 ccggcgcgat gggcgcgagc cagttcatgc cgtcgagcta ccgtgcgtac gccgttgacg
     4261 gcggcaatga caaacaacgc gacctgttcg ccgactggga cgacatcatc gccagcgtcg
     4321 ctaactactt caggaagcac ggctgggtga ccaatggccc ggtgctggcc gaagcctcgc
     4381 tgcagcccga cgcgccggtg accgccgatc ctggcaacct gtcgctcaat gaaaccgtcg
     4441 ccggcctgaa atccaagggc gtggacttcg acaccgcgca ggcggaaacc acgccggtgc
     4501 tgctgatccc cgcggaaacg gagctcgggc cctcctatcg cgtgggcttc aagaatttcg
     4561 aagtcatcac tcgttacaac cgcagcgtgc gttacgccat ggcggtgcac gatctcgcca
     4621 cgaccattgc cgatcgcgtg gcggcggcag gccctgctcc gggcggcgag accaagctgg
     4681 cggcgcagcc cggttcggcg gccgcgaccc ggtgatggga aatgtcccgc gcgcggcgat
     4741 tgcgctcaca ctgaccgcgt tcctactctc ggcctgcagt ctcgccaggc ggcagccgga
     4801 caagccgctg ccggacaagc aaagcaccag catcccgtcc gggtcggcgg tgccgcaacc
     4861 gcctcccgat gtcttctcga ttcccgatgc cgtgccgcga gccgagccgc gcggaacgcg
     4921 tggcaatccg ccgttctacg aagtttttgg caagcgctac tacgtgctgg cgagctccga
     4981 aggattcgtc gagcgcggca ccgcctcctg gtatggcccg ggctttcacg ccgcgtcgac
     5041 ctcgctgggc gaacgctacg acatgtacgc catgacggca gcgcacaaga ctttgcccat
     5101 cccggcctac gccgaagtca cgaacctgaa gaacggccgc aaggtcgtcg tacgcatcaa
     5161 tgaccgcgga ccgttcgtgg gtgaccgcat catcgacctc tcctacaccg cggcggccaa
     5221 gctcgacatg ctgctcgcgg gcactgcacc agttgaagtg cgcgtcatca cggcgcgcgg
     5281 cgccggatcg gcgctgccgc agccaggtgt gccagcgccc gtggctcccg cgttgccgcc
     5341 ggtggctgtg gtcaacgccg gtatcaaacc cggggcgccg gcgatgttca tccaggccgg
     5401 cgtgttctcg gatcacgaga atgcgcggcg gcgcgtcgaa gtgctgctcg cctccggcat
     5461 cgagctcgcg agcctggatg agatcccgag aaacgaacgc gcactgcatc gcgtgcgcgt
     5521 aggaccgttc gccagcgtcg aggaattcga tttcaacatg aaacggctgc gcgagctcgg
     5581 catcaacgac gcgcgcctgc tcaccgacta aaagggtgtc aggcaccgtt tcccaggaaa
     5641 cggtgcctga caccttttcc aggcgcgtac aatcgcgccc tgacgctttc ctcggagttc
     5701 catgcgatgc cgtcatcccg gctgatctcg taccttgcct gcattttcct tgctaacagc
     5761 gcgctcggcg ccgtgcccat tcccaaaccg ccggccatcg acgctcgtgc ctacgtgctc
     5821 atcgacttcc agagcggccg ggtgctggca gcggagaagg ccgacgtgcg gaacgaaccg
     5881 gccagcatca ccaaggtcat gaccgggtac ggggtgttcc gcgccgtgcg cgaaaagcgg
     5941 ctcagcctca ccgacagcgt caccatcagc gaacatgcat ggaaagccga aggctcgcgc
     6001 accttcgcgc aggtgggaac gcagattccg gtggacacgc tgatcaaggg catgatcgtg
     6061 cagtccggca acgacgccac catcgcgctg gccgagaagt taggcggcac cgaagacggc
     6121 ttcgtgcaga tgatgaacgc ctacgccgcc gagctcggcc tcaagaacac gcacttcgac
     6181 aacagctggg gcggcccggg gcccactcac tacatgagcg ccatggacat cgcgctgctg
     6241 gcgcgcatcc tgattcgcga ctacccggaa gagtacaagc tgtattcgct gcgcgagttc
     6301 gagtggaacg gcatcaagca gcagaaccgc aatggcctgc tggcgcgcga tccgtcggtc
     6361 gacggcatca agaccggcca caccgaaagc gcgggttatt gcctggcgag ctcggcaact
     6421 cgcaacggca tgcgactcat ctccgtcgtg ctgggcacgc agtcgttcaa ggcacgcgaa
     6481 gacgcgagcg ccgcactgct caactacggc tacacgttct acgaaaccgt gcaggtcaag
     6541 aaggccggtg acgtggtact caagcccgag gtttacaagg gcgaagaaga ccgcgtcacg
     6601 ctggttccgg cgcgcgacgt ctttgtcacc atcgggcgcg gctcggccgc gcagctcaag
     6661 accaccgcca ccgtgaaaac tccggtcatc gcgcccatca tcgccggcca gaccctcggc
     6721 gagctcaccg tatccgacgg caaggacatc atcgcgcgcg tgccgctggt cgcccaggcg
     6781 gccgtacccg aaggcggctg gtggacccgc atggtcgacg gaatttcgct ctggatggaa
     6841 taggattcga gagatgggcg aaccgctgcc gatctgcttc ctcaacgacg attttctgcc
     6901 gctgcgcgaa gcgcgcatct cgccgctcga tcgcggcttt ctgtattccg acggcgcgta
     6961 tgaagtcacg cccgtgtacg ccggccggcc gttccgtttc gagcagcatc acgcccgcct
     7021 gacgcgcagc ctcagggaaa tacgcatgga agatccgctg tcgcgcgaag cgtggcggga
     7081 tctgtaccgc gagctcatca gccgcaatgg cgccgaatcg tcagacgcct atatatatgt
     7141 gcaggtcacg cgcggcgcgg agcatggccg caaccatgcg ccactgccgg acgtgccgcg
     7201 caccgtgttc gcattcgtcg ccccctggcc caccggtcgg cccggcgcgt tcgagaacgg
     7261 catgcccgcc gtcacggccg atgacacgcg ctgggcgcgc tgcgacatca agtccgtgtc
     7321 gttgctggcc aacgtgctgc tgcgccagct cgcggtggat gaaaacgcca gcgagacaat
     7381 cctgattcgt gagggcttcc tcaccgacgc cagcgcttca agcgtgcacg tggtccaggg
     7441 cggcgtgctc tgtacgccac cgaattcctg gaagctgctc ccgggtacca cgcgcggggt
     7501 catcgaagaa attgcggaaa gggctggcgt catctggcgc tcggtggcga tatccgagcc
     7561 ggacctgcgc tcagcgtccg agatcttgtt aggcgcggcc acgcgcgaga tacagccggt
     7621 caccacactc gatggcaaac cggttggcga tggcaaaccc gggcccgtat ggcgcaggct
     7681 ctacgatgca taccaggcgt acaaacacga gatcgcgggg acaccgtggt agacgagatc
     7741 ccaccgagcg aggaattgct gaagttcccg acgagctacc ccatcaaggt ggtcggccgg
     7801 cgcagcgaac acatgcgcgc caacatcgac gccatcgtgc gcgcccacgt gcccgatctc
     7861 accgcggatc agatcaagga acgcgagagc acgcagcagc acttcctggc gatcacctac
     7921 accatcaacg cgcagagcaa agagcaggtc gtcgcgctcg cgcatgcgct gcaggcgagc
     7981 aaagacgtca tcatgctgat ctgacggcga atcgc
//
LOCUS       DASQJS010000166         8002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2292767, whole genome shotgun sequence.
ACCESSION   DASQJS010000166 DASQJS010000000
VERSION     DASQJS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8002
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2292767"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..741)
                     /locus_tag="VEW08_06965"
     CDS             complement(112..741)
                     /locus_tag="VEW08_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40511.1"
                     /translation="MTRVETLTVAGPAGPLETRLEFPDDSPAMPRVFGVACHPHPLFD
                     GTMNNKVTHVLARSMMECGAPAFRFNFRGVGASAGTFDNGRGETDDLAAVVAEGRRRF
                     PGAALWLGGFSFGAFVALRGATDLAPAKLVAVAPPVARFELGSVAHPDCDWMLAQGDA
                     DDVVPPEAVLAWAAEQPRKPRLHVLAGAGHFFHGKLHELKPLIIDFLKS"
     gene            complement(738..2024)
                     /locus_tag="VEW08_06970"
     CDS             complement(738..2024)
                     /locus_tag="VEW08_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ40512.1"
                     /translation="MRLLGGLALALLLGAGAASQAATLIHAGRVIDGVSDTVRTNQTV
                     VVVDGRITAIEAGFRAPAAGDRVIDLRQGTLLPGLFDMHVHLTSEYSKNSEIDTYKKN
                     EGDVAIDGVMYAQRTLLAGFTTVRDLGDSYRASIPLRNAINAGKVPGPRIVAAGKSIA
                     STGGHADPTNGWARKFPADPGPDDGVINSVEDARKAVRQRYKEGSDTIKITATGGVLS
                     IAKNGLNPQMTEEEIRAVVTTSRDYGFKVAAHAHGAEGIKRAVRGGVDTIEHGTFMDD
                     EGMKLMKERGTFYVATLSAGRWVFDQAQDPNFFPPIVRPKALQVGPQIQGTFAKAYKA
                     GVKFLFGTDTGVGKHGDNAREFKLMVDGGMPAMEAIRVATSVPARFLDLDDKVGSIVV
                     GKLGELVGVPGNPLEDITAMERVVFVMKDNTVFKAP"
     gene            complement(2024..2539)
                     /locus_tag="VEW08_06975"
     CDS             complement(2024..2539)
                     /locus_tag="VEW08_06975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HYJ40513.1"
                     /translation="MHRKSLANTVTMTLTALAVVACGRSEKPDSEEVPAPVAEAASAA
                     ASAPAATPSTGTEAMPLTKTDLTPGAGAEIKSGQNALVHYTGWLYDAAAPENKGKQFD
                     SSVGGEPFDFPLGGGRVIPGWDQGVVGMKVGGKRRLVIPPELAYGNAGAGGVIPPGAT
                     LVFDVELVEIQ"
     gene            complement(2585..4480)
                     /gene="htpG"
                     /locus_tag="VEW08_06980"
     CDS             complement(2585..4480)
                     /gene="htpG"
                     /locus_tag="VEW08_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HYJ40514.1"
                     /translation="MTTPQSDENKGKETLGFQAEVRELLKLMIHSLYSHREIFLRELI
                     SNASDANDKLRFEALSKPDLMAGDTELAISIEADAAKKTLTIRDNGIGMSREEAISHL
                     GTIARSGTAEFFKKLSGDQQKDSQLIGQFGVGFYSAFIVADRVEVLTRRAGLSASEGV
                     HWESAADGDFTVETLERAERGTTIVLHLKDDAKEFLDAWHLRSLVRRYSDHIAFPVRM
                     PKEGEATVEYEAVNRGTALWARSKAEIKDEEYVEFYRHLTHDAGEPLTWSHNRVEGKR
                     DYTSLVYIPGAAPFDLWQRDGARGLKLYVRRVFIMDDAEQFLPLYLRFVKGVLDSADL
                     PLNVSRELLQSDSEVEAMRAALTKRVLDLLARLSKDDAAKYQGFWNEFGAVLKEGLAE
                     DHGNRDKLLPLLRFASTNREGDAEEVSLSDYVARMQTGQDKIYYVVGESFAAARSHPA
                     IEGLRARNVEVLLLGKRIDAWVMDHLQDFEGKQFKDATRGDLDLGGLASAADKEKAEA
                     QLKENKPLLKRVKDALGERVSEVRVSTRLKDSAAVLVAGEHDISAPLRRVLEAAGQKA
                     PSGKPVLELNVEHHLVKYLENREDAADFGELALVLFEQALLAEGAQLPDPGAFVQRLN
                     RMWMRLQ"
     gene            4813..5595
                     /locus_tag="VEW08_06985"
     CDS             4813..5595
                     /locus_tag="VEW08_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HYJ40515.1"
                     /translation="MQRIVVLNPKGGSGKTTLAINLAAYFAGRGDATLLIDRDPQGSA
                     TRWLRKRKPPQPVITGIATFEHDARTTRSWQMRVPEGTEKVVVDSPAAVEARLMPELT
                     RDANKIIVPVLPSDIDIHAASRCIADLLLVAKIKRAENRIGVIANRVRKNTLMFQALM
                     RFLEKLEIPIVATMRDSQNYVRAAEQGVGIHEMKPYLVKEDMAQWQSLLAWLEPERVL
                     AEQPAPMNEQPAPEAVAVATEAAIEDTDGTAAHDARFITQAW"
     gene            5758..7911
                     /locus_tag="VEW08_06990"
     CDS             5758..7911
                     /locus_tag="VEW08_06990"
                     /inference="COORDINATES: protein motif:HMM:NF021021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HYJ40516.1"
                     /translation="MSRFRLPLILEICVPIMVLPGCGGDDAARTGVSEAAVMPTGTAP
                     VAISAGLTTFEDQWVRSKLQATDAEGDALTFSIVRAPDHSTLSLDPVTGAYELQPNAN
                     YFGTDAFEFAVSDGHGNAARAQVDVSVVPVRDPPVIDTRAMASVVAAGREAQLNFGIS
                     DPDGDAVALSVSQVGGTSVLPSLQVGEQVVRFVAPDVPAAESVELLFEAADPSGLSTR
                     TRKVITLSPVSQSGKLFTVLGSPQSAGMHWVITGDGFTAGEQQDLLRASITLAESVAG
                     VPELARHAKILNVHVLTAVSLDSGVTTAGASRTLRTAFDAIVGCADVERVACVNWGKV
                     YAALLSEQAPFDEVAVILNTSVYAGSGSNEGVVVSRNAYAPAIALHEMGHTMAGLADE
                     YIDDVAASAFVPGYREGQFPNVTTATDPARIPWRHWFADSTHLPDGPGDKGVGRFEGA
                     FYSASGFFRPKQDSIMRSLEGAVGEVNAEAWLRAMYRAVPPISAAYPMQSVVAGPAGA
                     TIEFELVSPWPPELVAIRWFIDGLEVDQARGAYRYALHADGGPHEVRVSVEDCSGSIR
                     APDAREHLGGFAWTVRNEPELQAHKAQPQSPRIGGWIRMRVDPTGHNVLGLSSSGPRR
                     VKVRQWADASDYEYALYDGGGAMVSQGRIADPRAIHGPLAPPGASETGHSVLTLQSGH
                     YLIGVPAGVDARRLRIRRFDGSMKKTAQSEQWLDL"
BASE COUNT         1397 a         2607 c         2555 g         1443 t
ORIGIN      
        1 ctgcccctga agagcctcaa ggcgctcgtg aactagcaat tgcgcgcgct acggcgcgtg
       61 catgcatgct acgaaagccc ggcatcgcaa ggtgcccggg cttttgtgca ttcaagactt
      121 cagaaaatcg atgatcaacg gcttcagctc gtgaagcttg ccgtgaaaga agtgcccggc
      181 gcccgccagg acatgcaatc gaggcttccg cggctgttcc gccgcccagg ccagtaccgc
      241 ctcgggcggc accacgtcgt ccgcgtcacc ctgcgccagc atccagtcgc agtccgggtg
      301 cgccacgctg ccgagttcga agcgcgccac gggcggggca actgccacca gcttcgccgg
      361 ggccagatct gtcgcgccgc gcagcgccac gaacgcgcca aacgagaatc cgcctaacca
      421 cagcgccgca ccggggaacc gccggcggcc ctcggccacg acggcggcaa gatcgtcggt
      481 ctcaccgcga ccgttgtcga acgtcccggc gctggcgccc acgccgcgaa aattgaaccg
      541 gaaggccggc gcgccacact ccatcatcga gcgtgccagc acgtgagtaa ccttgttgtt
      601 catcgttccg tcgaataacg gatggggatg acaggccaca ccgaacacgc gcggcatggc
      661 tggcgagtcg tcagggaact cgagtctggt ctccagcggc ccggcggggc ccgcgaccgt
      721 cagcgtctcg acgcgtgtca cggcgccttg aagacggtgt tgtccttcat cacgaacacc
      781 acccgctcca tggccgtgat gtcctcgagc ggattgcccg gcactcccac cagctcgccg
      841 agcttgccga cgacaatgct gcccaccttg tcatcgaggt cgaggaacct ggccggcacg
      901 gaagtcgcca cccggatcgc ctccatcgcc ggcatgccgc cgtcgaccat gagcttgaac
      961 tcgcgcgcgt tgtcgccgtg tttgccgacg cccgtgtcgg tgccgaagag gaacttcacg
     1021 ccggccttgt acgctttggc gaacgtaccc tggatctgcg gacctacctg cagcgccttc
     1081 ggtcgcacga tgggcgggaa gaagttcggg tcctgcgcct ggtcgaacac ccagcggccc
     1141 gcggacagcg tcgcgacgta gaacgtgccg cgctctttca tgagtttcat accctcatcg
     1201 tccatgaagg taccgtgctc gatggtgtcg acacccccgc ggacggcgcg cttgatgcct
     1261 tctgcaccgt gagcatgcgc tgccaccttg aagccatagt cgcgcgaggt cgtaaccaca
     1321 gcgcggatct cttcttcggt catctgcgga ttcagaccgt tcttggcgat cgacaggacg
     1381 ccgcccgtcg ccgtgatctt gatggtgtcg gagccttcct tatagcgctg ccgcacggcc
     1441 ttgcgtgcgt cctcgacgct gttgatgacg ccgtcatcgg gacccggatc ggccggaaat
     1501 ttgcgtgccc acccattggt ggggtccgca tgaccgccgg tgctggcgat ggacttgcct
     1561 gcggcgacga ttcgcggacc cggcaccttg ccggcgttga tcgcgttgcg cagtggaatc
     1621 gaggcgcgat aactgtcgcc aaggtcgcgc accgtggtga acccggctaa caacgtgcgc
     1681 tgcgcgtaca tcacgccgtc gatggcgacg tcaccttcat tcttcttgta ggtgtcgatc
     1741 tcggaattct tgctgtactc gctggtgaga tggacgtgca tgtcgaacag gccgggcagc
     1801 agcgtgcctt gcctgaggtc gattacccgg tcgccggcgg ccggcgcacg aaatcctgct
     1861 tcgatggcag tgatcctgcc gtcaaccacc acgacggtct ggttggtccg gaccgtgtcc
     1921 gagaccccgt cgatgacacg gcccgcgtgg atcagcgtgg cggcctggga ggcggcgcca
     1981 gcgccgagca gcagcgcgag cgcaagtccg cccagaagtc gcattactgg atttccacca
     2041 gctcgacgtc gaacaccagc gtcgcccccg gcggaatgac gccgccggcg ccggcattgc
     2101 cgtaggccag ttccggcggg atgacgaggc gtcgcttgcc gcccaccttc atgccgacca
     2161 cgccctggtc ccaacccgga atcacgcgtc cgcctccgag cgggaagtcg aagggttcac
     2221 cgcccaccga actgtcgaat tgtttgcctt tgttctcggg agcggccgcg tcgtacagcc
     2281 acccggtgta gtgcaccagc gcattctggc cggatttgat ttcggcgccg gcgccgggcg
     2341 tgaggtccgt cttcgtcaag ggcatggctt cagttcctgt agatggagtg gctgcgggtg
     2401 cagatgcggc cgcagacgcg gcttcagcaa ccggcgcggg cacctcttcg ctgtctggtt
     2461 tctcgctcct gccgcaggca acaacggcca gcgcggtcaa agtcatcgtg actgtattgg
     2521 ccagcgactt gcggtgcatc gcgagactcc ggtattgcgg gagcggcatt attgcaaaca
     2581 atctctactg caaccgcatc cacatgcgat tgagccgctg gacgaacgca cccgggtcgg
     2641 gaagctgcgc accttcggcg agcaaggcct gctcgaagag caccagcgcc agctcaccga
     2701 agtccgcggc gtcttcgcga ttctccaggt acttgaccag gtgatgctcg acgttcagct
     2761 cgagcaccgg tttgcccgaa ggcgcctttt gtccggccgc ttccagcacg cgacgcagcg
     2821 gcgcggagat gtcgtgttcg ccggcgacca gcacggcggc ggaatccttg agccgcgtgc
     2881 tcacgcgcac ctccgagacg cgctcgccga gcgcatcctt gacccgcttg agcaacggct
     2941 tgttttcctt gagttgcgct tcggccttct ccttgtcggc ggcgctcgcg agcccgccga
     3001 gatccaggtc gccgcgcgtc gcgtccttga actgcttgcc ttcgaagtcc tgcaggtggt
     3061 ccatgaccca cgcgtcgatg cgctttccta acaacagcac ctcgacgttg cgcgcgcgca
     3121 ggccctcgat ggcggggtgg ctgcgggccg ccgcgaaact ctcaccgact acgtagtaaa
     3181 tcttgtcctg gcccgtctgc atgcgcgcca cgtagtcgga gagcgagact tcctcggcat
     3241 cgccttccct gttcgtggaa gcgaaccgca acagcggcag caacttgtca cggttgccat
     3301 gatcctcggc caggccttcc ttgaggacag cgccaaactc gttccagaag ccctggtatt
     3361 tcgccgcgtc gtccttcgac agcctggcca gcaggtcgag cacgcgcttg gtcagcgcgg
     3421 cgcgcatggc ttccacttcg ctgtccgatt gcagcaattc tcgtgagacg ttcagcggca
     3481 ggtccgccga atccagcacc cctttgacga agcgcaggta cagcggcagg aactgctccg
     3541 cgtcatccat gatgaatacg cgccgcacgt acagcttgag cccgcgggcg ccgtcgcgtt
     3601 gccacaggtc gaacggtgcc gcaccgggga tgtatacgag actggtgtaa tcgcgcttgc
     3661 cctcgacgcg gttgtgcgac caggtcaatg gctcgcccgc gtcgtgcgtc aggtgacggt
     3721 agaactcgac gtactcctcg tccttgatct ccgccttgga acgcgcccag agcgccgtgc
     3781 cgcggttgac ggcctcgtac tcgaccgtgg cttcgccctc cttcggcatg cgcacgggga
     3841 aggcgatgtg gtccgaatac cggcgcacca gcgaacgcag gtgccaggca tcgagaaact
     3901 ctttcgcgtc gtccttgagg tgcagaacga tggtggtgcc gcgctctgcg cgctcgaggg
     3961 tctccaccgt gaagtcgcca tccgccgccg actcccaatg caccccttcg ctggcggaca
     4021 ggcccgcgcg ccgggtcagg acctcgaccc gatccgcgac gatgaacgcc gagtagaagc
     4081 ccacgccgaa ctggccgatg agctgcgaat ctttctgctg gtcgcccgag agcttcttga
     4141 agaactccgc ggttccggaa cgggcgatgg tgcccaggtg cgaaatggct tcttcgcggc
     4201 tcatgccgat gccgttgtcg cgaatggtca gtgtcttctt cgcggcatcc gcttcgatcg
     4261 agatggcgag ctcggtgtcg ccggccatca gatcgggctt gctcaaagcc tcgaaacgaa
     4321 gcttgtcgtt ggcgtcggag gcgttcgaga tcagctcacg caggaagatt tcccggtggc
     4381 tgtacagcga gtggatcatg agcttcagca gctcacggac ttcggcctgg aaacctaacg
     4441 tttccttgcc cttgttttcg tcggattggg gggttgtcat ggcgccggca gactgcgggc
     4501 gcgcaaatca gaattcaagc cctcggaggg gcctcgggaa tcaacggggg tcgatcagcg
     4561 cgggcggacg gacgtccaga tggccatcgc ggtgttcttc acgtagtacc ggctggtgcc
     4621 tacgaggtcg tcgatttcgt cccagagcag cagtagacgt tccatggagc cgatcgtagg
     4681 aacatgtctg cggcatcgct gtgtccgcag tcacagtcgt ccggtcgttt ggttgcgcca
     4741 tacgagcggc atcgctagtc tttggagtgc ttcacatcgc aaccacattc tgacaaacag
     4801 tccgaaggaa gcatgcaacg catcgtcgtc cttaacccta aagggggctc tggcaagacg
     4861 acgctcgcga tcaacctcgc ggcgtatttc gccggacgtg gcgacgccac cctgctcatc
     4921 gaccgcgacc ctcaagggtc ggccacgcgg tggctgcgca agcgcaaacc cccgcagccg
     4981 gtcatcaccg gcatcgccac cttcgagcac gacgcccgca ccacgcgctc ctggcagatg
     5041 cgcgtgccgg agggcacgga aaaggtcgtg gtggacagcc cggccgcggt tgaagcgcgc
     5101 ctcatgccgg agctcacgcg cgacgcgaac aagatcatcg ttccggtgct tccctcggac
     5161 atcgacatcc acgccgcttc gcgttgtatc gccgacctgc tgctggtggc caagatcaag
     5221 cgtgcggaga atcgcatcgg ggtaatcgcc aaccgggtgc gcaagaacac gctcatgttc
     5281 caggcactga tgcggttcct ggaaaagctc gagatcccga tcgtcgcgac gatgcgcgac
     5341 tcccagaact acgtgcgtgc cgccgagcag ggagtcggca tccacgagat gaaaccctac
     5401 ctggtgaaag aagacatggc ccagtggcag tcgctgctcg cctggctgga accggaacgg
     5461 gtgctcgcgg aacagcccgc ccccatgaac gagcagcccg cgcctgaagc cgtcgcggtt
     5521 gcgaccgagg cagccatcga agacaccgat ggcacggcgg cacatgacgc caggttcatt
     5581 acccaagcct ggtgatataa aacgacgacg ggaaacccgt agtctttcgc gcaaccgcgg
     5641 cgccatactg ccgcgcgcgt gatccagccc ccgacccgca gaaactcacg gctcgcgacg
     5701 cgcaaatctg tttgatcgcg cacgtccggg aattctcatc gatggcgtta tgcgcgcatg
     5761 tcgcgatttc gcctgcccct gatactcgaa atctgcgtgc cgatcatggt gctgcctggc
     5821 tgcggcggcg acgacgcggc cagaaccggc gtgtcggaag cggccgtgat gccgacgggc
     5881 accgcgccgg tcgcgatatc cgcaggtcta accactttcg aggaccagtg ggtgcgctcg
     5941 aagctgcagg cgaccgatgc agaaggtgac gccctcacct tttcgatcgt gcgcgctcca
     6001 gaccactcga ccctctcgct cgatccggtg accggtgcgt acgagttgca acccaacgcg
     6061 aactacttcg gtacggacgc attcgagttc gcggtcagcg acggccatgg caacgccgct
     6121 cgcgcccagg tcgatgtttc ggtggtgccg gttcgcgacc ccccggtgat cgacacccgt
     6181 gcaatggcgt cggtcgttgc cgccggacgc gaggcgcaac tgaatttcgg gatatccgac
     6241 ccggatggtg atgcggtggc tctctcggtc tcacaagtgg gcgggacttc agtgctgccg
     6301 agtctgcagg tcggcgagca agtagtgcga ttcgttgcgc cagacgtccc cgccgcagaa
     6361 tcggtggaac tgttgttcga agctgccgac cccagcggcc tctcgacccg cacgcgcaag
     6421 gtcataactc tgagcccggt gtcgcagtcg ggaaaattgt tcaccgtgct cggcagtccc
     6481 cagtccgctg gcatgcattg ggtgatcacc ggcgatggct tcaccgcggg tgaacaacag
     6541 gatctcctgc gcgcctcgat caccctggcg gagagcgtgg cgggcgtgcc cgagctcgcg
     6601 cgccacgcga agatcctgaa cgtccatgtg ctcaccgcgg tctcgctcga cagcggtgtg
     6661 acgacggcgg gcgcttcacg aacgttgcgg acggcgttcg atgccattgt gggttgcgcc
     6721 gatgtcgaac gagtcgcttg cgtgaactgg ggcaaggtct acgcggcctt gctgtcggaa
     6781 caggcacctt tcgacgaagt ggcagtgatc ctcaacacca gcgtctacgc tggaagcggt
     6841 tcgaatgaag gtgtcgtcgt ttcgcgcaat gcgtatgcgc cggcgatcgc gttgcacgag
     6901 atgggacaca cgatggccgg ccttgccgac gaatacatcg acgacgtcgc cgcgagcgca
     6961 ttcgtgccgg ggtaccgcga agggcaattt cccaacgtga ccacagccac cgatcccgcg
     7021 cgaattccat ggagacactg gttcgccgat tccacgcacc tccccgacgg acccggagac
     7081 aagggtgttg gccgattcga gggcgcgttc tattcggcaa gcgggttctt ccgtccgaag
     7141 caggacagca tcatgaggtc actggagggc gcggtcgggg aagtgaatgc cgaagcgtgg
     7201 ctgagagcga tgtaccgtgc cgtcccgccg atcagcgccg cctacccgat gcaaagcgtc
     7261 gtggccggac ccgcgggcgc cacgatcgag ttcgagctag ttagcccgtg gccgccggaa
     7321 ctggtggcga tacgctggtt catcgacggc ctggaagtcg atcaggcgcg cggcgcgtac
     7381 cggtatgcgt tgcacgccga cggtggcccg cacgaagtgc gggtgtctgt cgaggattgc
     7441 agcggcagca tccgggctcc ggacgcacgc gagcacctgg gtgggttcgc ctggaccgtg
     7501 cggaacgaac cggaactcca ggcgcacaag gcgcagcccc agtcgccgcg gattggtggg
     7561 tggatccgga tgcgcgtcga cccgaccggg cataacgtcc tggggttgag tagcagcggg
     7621 ccgcggcgcg tgaaggtccg gcaatgggcc gatgcatcag actacgaata cgcgctctac
     7681 gacggcggag gtgcgatggt ctcgcaaggc cggatcgccg acccgcgcgc gatccatggc
     7741 cccctggcgc cacccggggc gtcggagacg ggccacagcg tgctgacgct gcagagtggt
     7801 cactacctga tcggggttcc agccggagtc gacgcgcgca gactcagaat tcggagattc
     7861 gacggatcga tgaaaaagac ggcgcagagc gagcagtggc tcgatctgta ggcgggttat
     7921 ttcttggtgg gagccggaac aggctcgtct tcccaggaac ccttcacctt gtcggcccaa
     7981 tgcttgtagc tagaccaggt gt
//
LOCUS       DASQJS010000167        14365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1346046, whole genome shotgun sequence.
ACCESSION   DASQJS010000167 DASQJS010000000
VERSION     DASQJS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14365
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346046"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VEW08_06995"
     CDS             <1..1037
                     /locus_tag="VEW08_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HYJ40517.1"
                     /translation="ALLIGLFLWLGLMVRALLPALRRNDEQKPILTMLIISSTAIAGF
                     YGAALGYGRTTNLAVAEYWRWWVVHLWVEGFFEVFATAVIAFLFTRLGLLRVSTATVA
                     VLFATIVFLSGGVLGTLHHLYFTGTPTAVIALGASFSALEVVPLAFIGFEAYQTFKLG
                     SATPWMRRYRWPIMFFTAVAFWNLVGAGLFGFLINPPLPLYYMQGLNLTPLHGHTALF
                     GVYGFLGLGLMLFCLRGLKPNVQWNERALNTAFWSFNIGLAGMSLLTLLPIGLIQLNA
                     AIEHGYWYARSADLMQKPLIQTLVWMRVPGDIIFSVGAFSIAWFVLRLWVAPKQVAEE
                     LPVGTEPANR"
     gene            1067..1468
                     /locus_tag="VEW08_07000"
     CDS             1067..1468
                     /locus_tag="VEW08_07000"
                     /inference="COORDINATES: protein motif:HMM:NF016161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HYJ40518.1"
                     /translation="MIELYPEIKWVHIAAVLTSGGLFALRGVAMLAGARWYMAAPLRY
                     LSYTVDTVLLTAALMLATMLHQYPFVQSWLTVKVLLLVVYVVLGSYALKRGATRAART
                     LALGAALLVFAFIISVARAHDPRGVFALPGM"
     gene            1624..3126
                     /gene="nirK"
                     /locus_tag="VEW08_07005"
     CDS             1624..3126
                     /gene="nirK"
                     /locus_tag="VEW08_07005"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HYJ40519.1"
                     /translation="MARTTMTMLLAAALLLAGCGATDKTAPSSDVAADTGGSRKGDFG
                     PPQGEPVHAVLTSPPLVPPATNRKAPAKVIVELEVIEVDKQIAEGVNYTFWTFGGTVP
                     GSFIRVRQGDTVEFHLKNHPGSKMPHNIDLHGVTGPGGGAASSFTAPGHQSTFTFKAL
                     NQGLYVYHCATAPVGMHVANGMYGLILIEPPEGLSPVDQEFYVMQGDFYTVGKYREKG
                     HQAFDMQKAIDENATYVLFNGAESALTGPSALKAKTGQHIRMFVGNGGPNLVSSFHLI
                     GEIFDKVQYEGGTRYQENVQTTLIPAGGAVTVEFHTEVPGNYVLVDHSIFRAFNKGAL
                     AILEVSGEPRPEIYSGKQGDEVYLSDKLSNPEAVATAAVASASGTLTREQQVKAGEVL
                     FKGTCSACHQENGAGIENVFPPLAKSDYLLASRKRAIGVALNGLTGPVTVNGVSYNSV
                     MPPMSQLNDDEIANILTFTLNSWGNNGGVVTSAEVKAERANTKRTEGAAH"
     gene            3123..3908
                     /locus_tag="VEW08_07010"
     CDS             3123..3908
                     /locus_tag="VEW08_07010"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HYJ40520.1"
                     /translation="MRLAGNITLLFALLPGITAFAGGTGSVPIPGARFESVLPEAPGT
                     KIANVAPFRLDVSLVSNADFARFVRAHPEWRRGSVKPLLADAGYLQHWANANEPGESS
                     ARKPVTQVSWFAASAYCEARGSRLPRWHEWELAAAASDAVPDARADPAWRQRILAWYS
                     TSARGVLPDAGMSQANYYGVRDLHGVAWEWVLDAGSMMVSDDSREQGDPDLQRFCGSG
                     ALSLEQKENYALMMRIAMLSSMKASYTSGSMGFRCATDIEERR"
     gene            3905..4465
                     /locus_tag="VEW08_07015"
     CDS             3905..4465
                     /locus_tag="VEW08_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYJ40521.1"
                     /translation="MKVLLTCALLLAASLVSAASPEAAPGPSLYDLSERLSNQDGAPV
                     GLDVHRGNKVLVSMFYSSCPATCPLIIDTLRAVERGLDSSQRSDLRVLMISFDPARDT
                     AEALRELAKTRRIDTQRWTLARADDAAVRRIAAALEIQYRHLPDGQISHSNVITALGR
                     DGKILARSTEIGHADESLVRALATAP"
     gene            4632..5189
                     /locus_tag="VEW08_07020"
     CDS             4632..5189
                     /locus_tag="VEW08_07020"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYJ40522.1"
                     /translation="MQPTMYSSEASESGRPRRLRRVVQRSLRAPPRLARIAAMLIAAA
                     MVGTVSGASPSGPESLDLARHRGRVVIVDFWASWCKPCRQSIPWLNSMRERYGASGLT
                     IIGVNVDAERADADRFLRNVPIEFEIVFDPNGDLARQFEVQGMPSSYVFDRAGKMVET
                     HLGFREAKKDEKEAALRNLLNQPVR"
     gene            5203..5418
                     /locus_tag="VEW08_07025"
     CDS             5203..5418
                     /locus_tag="VEW08_07025"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HYJ40523.1"
                     /translation="MRKLILFVLACAALEGCASLGVQPWEREVLARDDMQLDTNALDT
                     AFDDHIYFSKEGTSGGRGFGGGGCGCN"
     gene            5420..6748
                     /locus_tag="VEW08_07030"
     CDS             5420..6748
                     /locus_tag="VEW08_07030"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HYJ40524.1"
                     /translation="MADKEMKIGAALAAATCSLLGTATPGVSIAADAEPKWDIESAFL
                     YYGESDDRVKDLSLSAHATRDFGDERTLGLDLSVDSLTGASPSGAIASGGAQTFTSPS
                     GRETYSTPAGEIPLDDTFLDTRFALNGSWSQPLARLYTVNAGLGFSSEYDYQHIGANF
                     GLTRDFNQRNTTLSAALAYAQDTVKPVGGIPLPLAQMTDVVDDDVSPGNRGDGSDTKN
                     VLDVLLGVTQVLGRHSVLRVNLSYSDSSGYLTDPYKILSVVDPVTGDLIGRTPAPGAS
                     GPTGVYLHESRPDSRRKEALYAELRHDFSGKVLQVGYRYSTDDWEVDSHTVESRLRLP
                     FGSSSYLEPHLRYYQQTAASFYRYSLANVTPLPGFASADARLSEMNALTFGLKYGHVN
                     AGGNEWNARLELYRQTAKAPNEVLIGNQIGNAQMPDFDAVILQFGYHFKL"
     gene            6793..7632
                     /locus_tag="VEW08_07035"
     CDS             6793..7632
                     /locus_tag="VEW08_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HYJ40525.1"
                     /translation="MGGPCEVLAEVADETEARKITESVAACAWRVENKFSRYRSDNIV
                     ARINTAGGKPVEVDVETANLLDFAALMHRLSDGLFDITSGVLRKAWTFDGGRRLPTQS
                     GIDALLPLVGWSKVRWVRPMIALSPGMEIDFGGIGKEYAVDQATQLAAQMSSAAVLVN
                     FGGDLAVTRSRKENRAWRVGIESIDAGDRRAAMLVDLAAGALSTSGDTYRYVESEGRR
                     LSHILDPRTGWPVNGAPRSVTVAAPTCTNAGMLTTLAMLRGPGAEAFLESEGVRHWVQ
                     RGT"
     gene            7725..8717
                     /locus_tag="VEW08_07040"
     CDS             7725..8717
                     /locus_tag="VEW08_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYJ40526.1"
                     /translation="MKAIRKILVAVKNPDARRQPAIDKAIRIAKTIGASIEFFHAIAE
                     PVFLEVQPLTGHTIAELKREALEIRQKRLDKLIAQARKLGVEAEGSVEWDFPPHEAIV
                     RRSVRCRADLIIAECHEGRRLGWLMRLTDWELLRASPVPVLLLKSARPWRRHIVLAAV
                     DPSHAHAKPSRLDSLIVEHAAQLAGALRGSLHVMHANYPQVLGLTLGDPAIDALTLAA
                     TYEQQKAKSREDFDLFAKKVKIPRGRRHVIDRDPVTGISTVARKVRAEVVVMGAVSRS
                     GLKRIFIGNTAERVLNSLPCDVLVVKPAHFEKRVAAKSRGMRMIAPQPLMPLPV"
     gene            complement(8744..9418)
                     /locus_tag="VEW08_07045"
     CDS             complement(8744..9418)
                     /locus_tag="VEW08_07045"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYJ40527.1"
                     /translation="MRKSEWREYSHMARNRTESLSPRKKPQQRRSRVTIDTIFEATIQ
                     VLLANGLDRITTIQIADRAGVSVGSLYQYFPNKRALMAAVVKRHVGQVADATIAACDS
                     MHGKSIGEMCATMMTAFVDAKTRRPEVSRALYLPSAAVNADAIVKEESIRCARAVHDM
                     LITASDAHFAQPQLVSGVLIASIVGPTRATIEAGGDRNTFERLKLHLTALCVGYLKEM
                     SAAAVD"
     gene            9429..10052
                     /locus_tag="VEW08_07050"
     CDS             9429..10052
                     /locus_tag="VEW08_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40528.1"
                     /translation="MHTYQYRFHFVLAVAGVLLAGQSAACEPPPGFVNPPRPEIAPLE
                     DLLSHTEVKEIARPLSAVMQAANRPLRIDATKDLAGVTGTFRLSNGPYGTVGARRLVC
                     KTDGTIAVEEVLLTESNGDGSRFRYVVWNHTGAKYRDVDYAVGEFVRSQPEPGKTLVN
                     WTYRFALKSGLSEEERSRFQKVFLERELSLWMRTQLDRDEPNAEAKP"
     gene            10225..10566
                     /locus_tag="VEW08_07055"
     CDS             10225..10566
                     /locus_tag="VEW08_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HYJ40529.1"
                     /translation="MAASAETAKAEAEPTMSFNVVYPNHEGARFDTSYYRSTHIPLVM
                     KVMKAANVMLIEGVPNGSSAAPYAMIAHFQFASSEAIKAALANPAMAEVRADVARFTD
                     IKPTIMLGKTT"
     gene            10663..11793
                     /locus_tag="VEW08_07060"
     CDS             10663..11793
                     /locus_tag="VEW08_07060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HYJ40530.1"
                     /translation="MLEQKINEMFGDDGPTRFGTGWWSGVLSAFFGMLAFGAVICLHF
                     PQLLSSPELRPYYPMDLMRLLIQAVIVGAILCGVISAMLRKKKVLALTGLLFALGATL
                     LGGSSVPINADLHGGPAIGLDWFLLDILLMTLIFSPIEVLWPAYPQQSVFRSEWLNDI
                     VYFLSTHLPIQITSFLILLPATQLTTRLNVAFISEAVGKLPWLVQFLLAILVADLAQY
                     WIHRGFHKISFMWRFHAIHHSSRALDWIAGSRAHLVEDVVVRGGILIPMMLIFPQSIN
                     VAYLFFVNFHATWAHCNFGPTVKWLEPFLIQPRFHHWHHTSQVESIDKNFAIHFPWID
                     KIFGTYYFPKDKWPDEYGLHNEKLPPGFWGQFFYPLTPRKSA"
     gene            complement(11790..12644)
                     /locus_tag="VEW08_07065"
     CDS             complement(11790..12644)
                     /locus_tag="VEW08_07065"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HYJ40531.1"
                     /translation="MKIYNAVGYACLLIYAVASAYFAPAHLGPWVGLAVGMAYLVVCW
                     FFAGLYLSCVIHMGIAHRALDYREWFVQSLTLVNNAFGVYIDPITWVNRHRLHHKYSD
                     HPGDPNKLAADGFWKTLHLCNFPYKTDVNVANDAILQTWPMRLAANPVFFVVTQAGNF
                     VLLWWLVGSLPFAALLWVGTRVFAVWVNMVQNYWTHEKRWGYRRYEDDDNAMNIGDWL
                     PVMATFSACLQNNHHHSPQFLRLSHEDSEFDFGFMTVKVMKKLGLVKASNYGAVLPKD
                     IPLKALEF"
     gene            12739..14319
                     /locus_tag="VEW08_07070"
     CDS             12739..14319
                     /locus_tag="VEW08_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40532.1"
                     /translation="MPLADIPIVVARDAEPAKARSVPPPARSASEPYRAYKPQPFTRE
                     ERDKVTILFGNLHWRAERVMQGVFSNLGYRTQILPTATRADLLAGREVADIGQCCPTS
                     FTTGNLVNTLRSEARRIGPEEVSKNFIYLTAGSCGACRFGQYHQSYELALRNSGLSGF
                     RMFLMGQGNLDQGAADGGGLDLNLPLLLGSAWSVLLADVVQDIEYQTRPYETKSGETV
                     RASKDAIEILHRAMLERPARGKKWRSLAWHLASPYFVNAMKDVRRRFDEVEVDRLRMK
                     PKVKITGEFYLQTVEGDPNYNIHRWLEAEGAEVYPAVIVIWFDYLMRMAAQDFEDHIG
                     INRGAYLKMKSIHTAQGLLRWTYGRMRNALGGVPHELPHQSTLRDLARPYYHSRLNGG
                     EGDMLVGKALWAHQKKKAHMICELSPYSCMPNTMSIGAMSAVVGKHPDLLYAPLEIKG
                     DAEVHALSRCQMILTEAKKRAQKEYDEALARTGKTANQLRERETAEMRRATYRVPRYG
                     VVGTAANYALHLAQCPPK"
BASE COUNT         2739 a         4458 c         4411 g         2757 t
ORIGIN      
        1 tcgcgctgct gatcgggctg ttcttgtggc tgggcttgat ggtgcgcgcg ctcttgcccg
       61 cgttgcggcg caacgacgag caaaagccca tcctcaccat gctcatcatc tcgtcgaccg
      121 cgatcgccgg cttctatggc gccgcgctcg gctacgggcg gaccacgaac ctcgcggtgg
      181 ccgagtactg gcgctggtgg gtcgtgcacc tgtgggtcga aggcttcttc gaagtgttcg
      241 cgaccgcggt catcgccttc ctcttcacgc ggcttggcct gctgcgagtc agcaccgcaa
      301 ctgtcgccgt attgttcgcg acaatcgtgt tcctgtccgg cggcgtgctg ggcacgctgc
      361 atcacctgta cttcacgggc acgcccaccg cggtcatcgc gctcggtgcg agcttctccg
      421 cgctcgaagt ggtgccgctg gcgttcatcg gcttcgaggc ctaccagacc ttcaagctgg
      481 gcagcgcgac gccgtggatg cggcgctatc gctggcccat catgttcttc accgccgtcg
      541 cgttctggaa cctggtgggc gcgggcctct tcggcttcct catcaatccg ccgctgccgc
      601 tctattacat gcagggactc aatctcacgc cgctgcatgg ccacaccgct ttgtttggtg
      661 tgtacggctt cctcggcctt gggctgatgc tgttctgcct gcgcgggctc aaacccaacg
      721 tgcagtggaa cgagcgcgcg ctcaacacgg cattctggtc gttcaacatc ggcctggccg
      781 gcatgtcgct gctcacgctg ctgcccatcg gcctcatcca gctgaacgcg gcaatcgagc
      841 acggctactg gtacgcgcga tccgccgacc tcatgcagaa gcctctcatc cagacgctgg
      901 tgtggatgcg ggttcccggc gacatcatct tcagcgtcgg cgctttcagc attgcctggt
      961 tcgtcctgcg actctgggtc gcaccgaagc aggtggcgga agagctgccg gtggggactg
     1021 agcccgccaa ccgctgagaa cgcgaaggaa tggtcagaat cgccgcatga tcgagctcta
     1081 tcccgagatc aagtgggttc acatcgcggc ggtgctgacc agcggtgggc tgtttgcgct
     1141 gcgcggtgtc gcaatgctcg ccggagcgcg gtggtacatg gcagcgcccc tgcgttacct
     1201 gagctatacg gtcgacacgg tgttgctcac cgcggcactc atgctcgcga cgatgcttca
     1261 tcagtatcca ttcgttcagt cgtggctcac cgtgaaggtg ctgctgctgg tggtgtacgt
     1321 ggtactgggc agttatgcgc tgaagcgcgg tgcgacgcgg gctgccagga cgcttgccct
     1381 cggggccgcg ctgctggtgt tcgcgttcat catcagcgtc gcacgcgcgc acgatccgcg
     1441 cggtgtgttc gcgctgcccg gaatgtgaac ctttcgggtc gggtcgcgcc ctggtcctac
     1501 gcagtcccgc cgcaagtcgc gggttactac gtatacacgc gtcagccgat ctgaatcaga
     1561 gtgcttgccg tcaccgcgcg ctaactatca tggcgacggg caaactaact cgaggttcct
     1621 tacatggcac ggactacgat gacgatgttg ctcgcggcag cgctgctgct tgccgggtgc
     1681 ggcgcaacag acaaaaccgc gccctcaagc gacgtggccg ccgataccgg tggctcgcgc
     1741 aagggcgact tcggtccgcc gcagggtgaa cccgtccacg ccgtgctcac cagtccgcca
     1801 ctggttccgc cggccaccaa tcgcaaggcg ccggccaaag tcatcgtcga gctcgaagtc
     1861 atcgaagtcg acaagcagat cgccgaaggc gtgaactaca ccttctggac tttcggaggc
     1921 acggtccccg gcagcttcat ccgcgtgcgc cagggcgata cggtcgagtt tcacctcaag
     1981 aaccatccgg gcagcaagat gcctcacaac atcgacctgc acggagtcac cgggcccggc
     2041 ggcggcgccg catcgagctt cacggctccg ggacatcagt cgacgttcac cttcaaggcc
     2101 ctgaaccagg gtctgtacgt ctatcactgc gccaccgcgc ccgtgggtat gcacgtggcc
     2161 aacggcatgt acggcctcat tctgatcgag ccgcccgaag gcctgtctcc cgtcgaccag
     2221 gagttctacg tgatgcaggg cgacttctac accgtcggca agtaccgcga gaaaggtcac
     2281 caggccttcg acatgcagaa ggctatcgat gaaaacgcga cctacgtgct gttcaatggc
     2341 gccgaatcgg cgctcacggg accgagcgcg ctcaaggcga aaaccggcca gcacattcgc
     2401 atgtttgtcg gcaacggcgg tccgaacctg gtctccagct tccacctgat cggcgaaatc
     2461 ttcgacaagg tgcaatacga aggcggcacg cgataccagg aaaacgtgca gaccacgctg
     2521 attccggccg gtggcgcggt caccgtcgag tttcacaccg aagtccccgg caactacgtg
     2581 ctcgtcgatc actcgatttt ccgtgccttc aacaagggcg ccctcgccat tctcgaagtg
     2641 agcggcgagc cgcgaccgga gatctactcc ggcaagcagg gtgacgaggt gtacctgagc
     2701 gacaagctgt cgaatcccga ggcggtcgcg accgccgcgg tggcgagtgc cagcggcaca
     2761 ctcaccaggg aacagcaggt gaaggccggt gaggtgttgt tcaagggcac gtgttcggca
     2821 tgccaccagg agaatggcgc cggcatcgaa aacgtcttcc cgccgctcgc caagtctgac
     2881 tatctactgg cgagccgcaa gcgcgcgatc ggcgttgcgc tcaatggcct cacgggcccg
     2941 gtgaccgtga acggcgtgag ctacaactcg gtgatgccgc cgatgagcca gctcaacgac
     3001 gacgagatcg cgaatatcct gacgttcacg ctcaacagct ggggcaacaa cggcggcgtg
     3061 gtcaccagtg ccgaagtgaa agcggagcgc gcgaacacca agcgcaccga gggagccgcg
     3121 cattgaggct cgctggcaac atcacgttgc tattcgcgct gctgccaggt atcacggcct
     3181 tcgctggcgg caccggcagc gtgccgattc ccggcgcgag attcgaatcg gtgctccccg
     3241 aagcgcccgg aacgaagatc gcgaacgtcg caccgttccg gctcgacgtt tcgctcgtga
     3301 gcaatgcgga cttcgcgcgt tttgttcgcg cacatccgga gtggcgtcga ggctccgtca
     3361 agccgttgtt ggcggacgcc ggatatcttc agcattgggc gaacgccaac gagccgggcg
     3421 aaagttccgc ccgcaagccc gtgacgcagg tgagctggtt cgccgccagt gcctactgcg
     3481 aagcccgcgg ctcgcgtctg ccccgctggc atgaatggga actcgcggca gcggcgagtg
     3541 acgccgtgcc tgacgcacgg gccgatcccg cgtggcggca gcgcattctc gcctggtact
     3601 cgacatcggc gcgcggtgtg ctgccggatg cgggcatgtc acaagccaac tactacggcg
     3661 tgcgcgacct gcacggagtt gcctgggaat gggtgctgga cgcgggctca atgatggtca
     3721 gtgacgacag ccgcgagcag ggtgatccgg atctgcagcg cttctgcggt agcggcgcgc
     3781 tgagtctcga acagaaggag aactacgcgc tgatgatgcg tatcgccatg ttgtccagca
     3841 tgaaagcctc gtacacgtcg ggatcgatgg gattccgctg cgcaaccgat atcgaggaac
     3901 gccgatgaaa gtccttttga cctgcgctct cttgctggcg gcgagcctcg tgagcgccgc
     3961 atctcccgaa gcggcccccg gtccgtcgct gtacgacctg agcgagcgac tctcgaacca
     4021 ggacggcgcg ccagtgggcc tcgacgttca tcgcggcaac aaggtgctgg tgtcgatgtt
     4081 ctactcgagt tgccccgcaa cctgcccgct gatcatcgac accctgcgcg cggttgagcg
     4141 tgggctcgat tcttcccagc gctccgacct ccgggtgctg atgatttcct tcgacccagc
     4201 acgtgatacc gccgaggcgc tgcgcgagct cgcaaagaca cggcgtatcg acacccagcg
     4261 ctggacgctt gcgcgcgccg acgacgctgc ggtccggcga attgccgcgg cgctcgagat
     4321 ccagtacagg cacctgcccg atgggcaaat cagccattcg aacgtcatca cggcgctcgg
     4381 acgcgatgga aaaattctcg cccgcagcac cgagatcgga catgcggatg aaagcctggt
     4441 gcgcgcgctc gcgactgccc cctgatcgcg cccccggagc ggtccgattg cgcggtgcga
     4501 ttttggacac gcgcaaccta ttgaatatat tggtcacgcg attaaattcc ggggaaccgc
     4561 ggttgccgcg acggagtttg ggatcggcgg tcgtctaacc cgccagtgca atccttccgc
     4621 aaggacgatt cgtgcagcca acgatgtatt cgagcgaagc cagcgagagc gggcgaccgc
     4681 ggcgtctccg ccgcgtggtc cagcgttcat tgcgtgcgcc gccgagactt gcgcgcatcg
     4741 cggcgatgct gattgctgcc gccatggtcg gcacggtttc cggagcgtcc ccctccggtc
     4801 cggagtccct cgatcttgcg cgccatcgcg gccgtgtggt catcgtggat ttctgggctt
     4861 cgtggtgcaa gccatgtcgc cagtcgatcc cctggctcaa ctccatgcgc gaacgttacg
     4921 gtgccagcgg cctgaccatc atcggcgtga acgtggacgc ggagcgtgct gacgccgatc
     4981 gcttcctgcg aaacgttccc atcgaatttg agatcgtttt cgatcccaac ggcgacctcg
     5041 cgaggcaatt cgaagttcag ggcatgccca gttcctatgt cttcgatcgc gccggcaaga
     5101 tggtcgaaac ccaccttgga tttcgcgagg caaagaagga tgagaaggaa gcggcactca
     5161 gaaatctgct gaatcaacct gtccgatgat caaggaaatc tcatgcgtaa gttgatcctg
     5221 ttcgtgctcg cctgcgctgc ccttgaagga tgcgccagtc ttggtgtgca gccatgggag
     5281 cgcgaagtcc tggcccgcga tgacatgcag ctcgatacca acgcgctcga cacggcgttc
     5341 gacgatcaca tctatttcag caaggaaggc accagtggcg ggcgcggctt cggcggcggc
     5401 ggctgcgggt gtaactgaca tggctgacaa ggagatgaag atcggcgccg cgcttgcggc
     5461 ggccacctgc agcttgttag gcacggcgac gcctggagtt tccattgcgg cggacgcgga
     5521 acccaagtgg gacatcgaat ccgcgtttct gtactacggt gaaagtgatg atcgcgtaaa
     5581 ggatctgagc ctgagcgcgc atgccacccg cgatttcggc gacgagcgaa cactcggcct
     5641 cgacctctcc gttgactcgc tcaccggtgc ctcgccgagt ggcgcgatag ccagcggtgg
     5701 cgcacagact ttcaccagcc cgtcgggccg cgagacttac agcacgcccg cgggtgagat
     5761 ccctctcgac gacacctttc tcgacacccg gttcgcgctc aacggcagct ggtcgcagcc
     5821 gctcgcacgt ctctacactg tgaacgccgg actcgggttc tcttcagagt acgactacca
     5881 gcacatcggc gcgaacttcg gtctgacgcg cgatttcaac cagcgcaata ccacgctgag
     5941 cgcggcgctc gcgtacgccc aggacaccgt caagcccgtc ggtggaatac ctctgccgct
     6001 ggcgcaaatg accgatgtcg tggatgacga cgtctcgccg ggcaatcgcg gcgatggcag
     6061 cgataccaag aacgtcctcg acgtgctgct cggagtcacc caggtgctcg gccggcactc
     6121 ggtactgcgc gtcaatctct cgtattccga ttcgagcggt tatctcaccg atccttacaa
     6181 gatcctcagt gtcgtcgacc ctgtcaccgg cgatctcatt gggcgaacgc cggcgcccgg
     6241 cgcatccggt cccactggcg tttatcttca tgagagccgc ccggattcgc gccgcaagga
     6301 agcgttgtac gcggagctgc gccacgactt ttccggcaag gtgctgcagg tgggctatcg
     6361 ttactccacg gacgactggg aagtggattc gcacacggtg gaatcgcgcc tgcgactgcc
     6421 gttcggatcg tccagctatc tcgaaccaca cctgcgctac taccagcaga ccgcggcgtc
     6481 gttctatcga tacagcctgg cgaatgtcac gcccctgccc ggcttcgcct cggccgacgc
     6541 tcgcctgtcc gagatgaacg cactcacgtt tgggctcaag tacggccacg tgaatgccgg
     6601 aggcaacgaa tggaacgcga gactggaact ttaccggcag acggccaagg ctccgaacga
     6661 agtgctgatc ggcaatcaga tcggcaacgc gcagatgccg gacttcgacg cggtgatcct
     6721 gcagttcggt taccacttca agttgtagag ctttcacacg acgacgaact gtggataggc
     6781 cgttttcgcg ccatgggcgg gccttgcgag gtactcgcgg aagtggccga tgagacggag
     6841 gcacgcaaga ttaccgaaag cgtggctgcc tgtgcgtggc gcgtcgagaa caagttcagc
     6901 cgttatcggt cggacaacat cgtcgcgcgc atcaataccg cgggcggcaa gcccgtcgaa
     6961 gtcgacgtcg aaaccgcgaa cctgctcgat ttcgcggcgc tgatgcaccg gttatccgat
     7021 ggcctgttcg acatcacctc gggcgtgttg cgtaaggcat ggacctttga tggcggcagg
     7081 cgattgccca cgcaatccgg gatcgacgca ctgcttccgc tcgtgggctg gtcgaaggtt
     7141 cgctgggtcc gcccgatgat cgccttgtct cctggtatgg agatcgattt cggcggcatc
     7201 ggcaaggaat atgcggtcga tcaggcgacg caacttgcgg cgcaaatgag cagcgccgcg
     7261 gtactcgtca actttggcgg cgatctcgct gtcacacgtt cgcgcaagga gaatcgggcg
     7321 tggcgcgtcg gcatcgaaag catcgatgca ggcgaccgac gcgccgcgat gctggtggac
     7381 ctggcggccg gtgcgttatc aaccagcggc gatacttacc ggtatgtcga gtcggagggc
     7441 cggcgactgt cacacatcct cgatccgcgg accgggtggc cggtgaacgg cgcaccgcgc
     7501 tcggtcaccg tcgccgcgcc cacgtgcacc aacgcgggca tgctcaccac gctcgccatg
     7561 ctgcgcggac cgggggccga agcattcctg gaatccgagg gggtgcggca ctgggtgcag
     7621 aggggtacgt agaactccgg acaggttagg cgttgctacg aatcgcgaga tcgcccggtc
     7681 gcgcccacga tgcgttcctc aggagcaaca ccgaggatcg caatatgaaa gccatccgca
     7741 agattctggt agcggtgaag aacccggatg cgcgtcgcca gccagccatc gacaaggcca
     7801 ttcgcattgc aaagacgatt ggtgcgtcga tcgaattttt tcatgcgatt gcggaaccgg
     7861 tgtttctcga agttcagcct ctcaccggcc ataccatcgc ggagctcaag cgcgaggcgc
     7921 tcgagatccg gcagaagcgg ctggacaagc tgattgcgca ggcgcgcaag ctcggtgtgg
     7981 aagctgaggg ttcagtcgaa tgggatttcc ctccgcacga agcaatcgtc aggcgatcgg
     8041 tacgctgccg cgcggatctg atcattgccg aatgtcacga agggcgacga ctggggtggc
     8101 tgatgcgtct cacggactgg gagcttttgc gggcaagccc ggtacccgta ttgctgctca
     8161 agagtgcccg gccgtggcgc cggcacatcg tgcttgctgc cgttgaccct tctcatgctc
     8221 atgcgaagcc atcccgcctg gattcactga tcgtcgagca tgccgcgcag ctcgccggtg
     8281 ctctgcgtgg ctcattgcat gtgatgcacg cgaactatcc acaggtgctt ggcttgacgc
     8341 tgggagaccc ggcaattgac gctttgacac tcgcggcgac gtacgaacag cagaaggcca
     8401 agagtcgaga agacttcgac cttttcgcca aaaaagtaaa gataccgcgt ggtcgacggc
     8461 acgtgatcga ccgcgacccg gtgacgggaa tttccaccgt ggcgcgaaag gtgcgagccg
     8521 aagtggtggt gatgggtgcc gtatcgcgtt ccggattgaa aagaatattc attggcaaca
     8581 ccgcggagcg ggtactcaac agtttgccct gcgacgtcct ggttgtgaaa ccggcgcatt
     8641 tcgagaaacg tgtggctgcg aagtctcgcg gcatgcgcat gatcgcgccg cagcctctca
     8701 tgccgctgcc ggtctgaggg ttccgccgaa cggagcgtgg atactagtca accgcagcag
     8761 ccgacatctc ttttagatag cctacacaca gcgcggtgag gtgcagcttc agtctttcga
     8821 acgtgttgcg atcaccacca gcctcgatgg tcgcccgcgt gggtcccacg atggatgcga
     8881 tcaacacgcc actcaccagt tgtggctgcg caaagtgcgc gtcactggcc gttatcaaca
     8941 tgtcgtgcac tgcccgggca cagcgaatcg actcctcttt gacgatggca tcggcattca
     9001 ccgccgcgga cggcaggtag agcgcgcggg atacttccgg cctgcgagtc tttgcatcga
     9061 cgaaggcggt catcatcgtc gcgcacattt cgccgatcga ctttccatgc atcgaatcgc
     9121 aagccgcgat cgtggcgtca gcaacctgac cgacatgccg ctttacgact gccgccatca
     9181 gggcacgttt gttcggaaag tattggtaga gagaaccgac ggaaacgccg gcccggtcgg
     9241 caatctggat agtggtgatc ctgtcgagcc cgttggcgag caggacctga atagtcgctt
     9301 cgaaaatagt atcgatggtg acgcgcgacc gtcgctgttg cggctttttc cttgggctca
     9361 gcgattcggt tcggttgcgt gccatatgcg aatattcccg ccattccgat ttgcgcaaga
     9421 tacctcgcat gcacacatac caatatcgat ttcacttcgt gctggccgtc gccggcgtgc
     9481 tgctggcagg ccagagcgcg gcttgcgaac cgccgcctgg attcgtcaat ccgccgcgtc
     9541 ccgaaatcgc gccgctcgag gatctgttga gtcacacgga ggtgaaggag attgccaggc
     9601 cgctctcggc cgtcatgcag gcggccaatc gtccgctgcg gattgatgcg acgaaagacc
     9661 tggccggtgt caccggcacc ttcaggctgt cgaacggccc ctatggcact gtcggcgcac
     9721 gccggcttgt ctgcaaaact gacggaacca tcgccgtcga agaagtgctg ctcacggaat
     9781 cgaacggcga tggctcacgt ttcaggtatg tggtctggaa ccacaccggt gcgaagtatc
     9841 gtgacgtcga ctatgccgtg ggtgagttcg tccgctcgca acccgagccc ggcaagactc
     9901 tggtcaactg gacgtatcga ttcgctctca agagcgggct cagcgaagag gaaaggtccc
     9961 gattccagaa ggtctttctc gagcgcgagc tctcattgtg gatgcgcacg caactagacc
    10021 gggatgaacc aaatgccgag gccaagccct gaacggacgt cagtctgcag tcgcgaggcg
    10081 cggagtcatg acctgttcgg cgaaattgtt tcgtcgacgc ggaaccccta tccttcagcg
    10141 acgactcttc tcaccagacg ggagatgaca acgatgttga acaggcgcgg agttattttg
    10201 ggcgtggcga ctggtgttgc cgcgatggcg gcctcggccg agacggcaaa ggcggaagcg
    10261 gaaccgacga tgagcttcaa cgtggtatac ccgaatcacg agggtgcacg tttcgacact
    10321 agctactatc ggtccacgca catcccgctg gtgatgaagg tcatgaaagc cgccaacgtc
    10381 atgctcatcg agggcgtccc gaacggtagt tcggccgcac cgtacgcgat gattgcccac
    10441 tttcaattcg cctcgtccga ggccatcaag gccgctctcg ccaatcccgc aatggccgaa
    10501 gtccgggccg atgtcgccag gttcaccgac atcaagccga cgatcatgct gggcaaaacg
    10561 acgtagagcc gacgaatcgt acgtagatcg catttattgc acggaaccgt gtgtcgcggc
    10621 cgggattgca gtagttttcg gcctggtcaa cgacccaaac tgatgctcga acaaaaaatc
    10681 aatgagatgt tcggcgacga cggccccacc aggttcggaa ccgggtggtg gagcggcgtc
    10741 ctgtcggcgt tttttggaat gctggcattc ggggcggtta tctgcctgca cttcccccaa
    10801 ctgctctcct cccccgagct tcgcccttac tacccgatgg acctgatgcg cctgttgatc
    10861 caggcggtca tcgtgggcgc aattctctgc ggcgtgatct ccgcgatgtt gcgaaagaag
    10921 aaggtcctcg ctttgacggg gttgctattc gcgctgggcg cgactctgct gggcggatcg
    10981 agtgttccta ttaatgccga tctgcacggt ggtccggcaa tcggcctgga ctggtttctg
    11041 ctcgacattc tgttgatgac gctcatcttt tctccgatcg aagtcctttg gcccgcctat
    11101 ccgcagcaga gcgtctttcg cagcgagtgg ctgaacgaca tcgtgtattt cctgtccacg
    11161 catttgccga tccagatcac gagtttcctg atcctgctgc cagccacaca gctcacgaca
    11221 aggctgaacg tcgcgttcat ttcggaggcg gtgggcaaac taccctggct ggtgcagttc
    11281 ctgctggcga tcctggtcgc ggacctggcc cagtactgga ttcatcgcgg tttccacaag
    11341 atttcattca tgtggcgctt tcatgcaatc caccattcct ccagggcgct cgactggatt
    11401 gcgggctcac gcgcccatct cgtggaagac gtcgtggtga ggggcggcat cctcattccg
    11461 atgatgctga tatttccgca aagcatcaac gtggcctatc tgttcttcgt gaacttccat
    11521 gccacctggg cgcattgcaa tttcggcccg acggtcaagt ggctggagcc gttcctgatc
    11581 cagccacgtt tccaccactg gcatcacacc tcgcaggtgg aatccatcga caagaacttc
    11641 gcgatccact ttccgtggat cgacaagatc ttcggaacgt attacttccc gaaggacaag
    11701 tggccggatg aatacggcct gcacaacgag aagctaccgc ccggtttctg ggggcagttc
    11761 ttctatcctt tgacgccgcg gaagtcggcc tagaattcca gcgccttcaa cggaatgtcc
    11821 ttcggcaaca ccgcgccgta gttcgaagcc ttcaccagcc ccagtttctt catgaccttg
    11881 accgtcatga aaccgaagtc gaactccgaa tcctcgtggc tcagccgcag gaattgcggc
    11941 gaatggtggt ggttgttctg caggcacgcg ctgaaggtgg ccatcacggg caaccagtcg
    12001 ccgatgttca tcgcattgtc gtcgtcctcg tagcggcgat atccccagcg cttttcgtga
    12061 gtccagtagt tctggaccat gttgacccac accgcgaaca cgcgcgtgcc aacccacagc
    12121 agcgcagcga acggcaggct gcctaccagc caccacagca gcacgaagtt gcctgcctgc
    12181 gtcaccacga agaacaccgg gttcgccgcg aggcgcatgg gccaggtctg gaggatggcg
    12241 tcgttggcca cgttgacgtc ggtcttgtaa gggaaattgc acaggtgcaa ggttttccag
    12301 aagccgtcgg cggcgagctt gttagggtcg ccgggatgat ctgaatactt gtggtgcagg
    12361 cgatggcgat tcacccaggt gatgggatcg atatagacgc cgaaggcatt gttcacgagc
    12421 gtgaggctct ggacgaacca ttcccggtag tcgagcgcac gatgcgcgat gcccatgtga
    12481 atgacacagg acaagtacag ccctgcgaag aaccagcaca ccacgagata ggccatgccg
    12541 accgccagac ccacccaggg accgagatgc gccggcgcga agtacgcgga agccaccgca
    12601 tagatcagaa gacacgcata cccgaccgcg ttatagatct tcatttactc cctcccgaaa
    12661 gttggccgcc aaattctacg tcgcgtagcg gatcattgag gcagtattgg cgtcaaggca
    12721 ttcgtgggag agctagctat gccgcttgct gatattccaa tcgttgtcgc cagagacgcc
    12781 gagcccgcga aagcgcgcag cgtcccgccg ccggcccgaa gtgcctcgga gccctaccgg
    12841 gcttacaagc cacaaccctt tacccgcgag gaacgcgaca aggtcacgat tcttttcgga
    12901 aacctgcact ggcgcgcgga gcgggtcatg caaggtgtgt tttcgaacct cggctaccgc
    12961 acgcagatat tgccgaccgc gacgcgcgcg gacctgctcg cggggcgcga agtcgccgac
    13021 atcggtcaat gctgtcccac cagtttcacc accggcaacc tcgtcaatac tctgcgcagc
    13081 gaagccaggc gcatcgggcc tgaggaagtc tcgaagaact tcatctacct cactgccggt
    13141 tcctgcggcg cctgccgctt cggtcaatac caccagagct atgaactcgc gctgcgcaac
    13201 tccggccttt ccgggttccg catgttcctg atggggcagg gaaatctcga ccagggtgcg
    13261 gcagacggcg gcgggctcga tctcaatcta ccgctccttc tcgggtctgc atggtcggtg
    13321 ttgcttgccg acgtggtgca ggacatcgaa taccagaccc gtccctatga gacgaagtcg
    13381 ggcgaaaccg tgcgtgcctc gaaggacgcc atcgaaattc tgcatcgggc gatgctcgag
    13441 cggcctgcgc ggggcaagaa atggcgttcg ctcgcctggc acctggcctc gccttacttc
    13501 gtcaatgcga tgaaagacgt ccgccggcgc ttcgacgagg tcgaggtcga tcggctgcgc
    13561 atgaagccca aggtgaagat caccggcgag ttctacctgc agaccgtgga aggcgacccc
    13621 aactacaaca ttcaccgctg gctggaagcg gaaggtgcgg aggtctaccc ggcggtgatc
    13681 gtcatctggt tcgactacct gatgcgcatg gccgcgcagg atttcgaaga ccacatcggc
    13741 atcaaccgcg gcgcatatct gaagatgaaa tcgattcaca ccgcgcaggg attactacgc
    13801 tggacctatg gccgcatgcg caacgcgctc ggcggtgttc cgcacgagct gccgcaccag
    13861 tccacgttgc gcgatctcgc caggccctac taccacagcc ggctgaacgg cggcgaaggc
    13921 gacatgctgg tcggcaaggc gctgtgggcg caccagaaga agaaggcgca catgatctgc
    13981 gaactctctc cctactcctg catgccgaac accatgagca tcggagcgat gtcggcggta
    14041 gtcggaaagc atccggacct gctgtacgca ccgctcgaga tcaagggcga cgccgaagtg
    14101 catgcgctgt cgcgctgcca gatgatcctg accgaagcga agaaacgcgc gcagaaggaa
    14161 tatgacgaag cgttggcgcg caccggaaaa accgcgaatc aattgagaga acgcgagacc
    14221 gcggaaatgc ggcgcgccac ctaccgtgtg ccgcgctacg gcgtggtggg tacggcggcc
    14281 aactacgcac tgcatctggc gcaatgcccc ccgaaatgat gatcgttggc ctcgacgtcg
    14341 ggtccaccac cgtcaaagcg gcggt
//
LOCUS       DASQJS010000168         4189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1979071, whole genome shotgun sequence.
ACCESSION   DASQJS010000168 DASQJS010000000
VERSION     DASQJS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1979071"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VEW08_07075"
     CDS             complement(<1..314)
                     /locus_tag="VEW08_07075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="catalyzes the transcription of DNA into RNA using
                     the four ribonucleoside triphosphates as substrates.
                     Dimerization of the alpha subunit is the first step in the
                     sequential assembly of subunits to form the holoenzyme;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HYJ40533.1"
                     /translation="MQASVGEFLKPRVVKVTPTTAKQAKVVIEPFERGFGHTLGNALR
                     RVLLSSMPGCAITEAEIDGVLHEYTSIEGVQEDVVDILLNLKQVAIRLNAREETQLRL
                     HK"
     gene            complement(401..1024)
                     /gene="rpsD"
                     /locus_tag="VEW08_07080"
     CDS             complement(401..1024)
                     /gene="rpsD"
                     /locus_tag="VEW08_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HYJ40534.1"
                     /translation="MARYLGPKCKLSRREGTDLFLKSGVKPLESKCKLEVPPGGVKGE
                     RRGRLSDYGVQLREKQKLRRMYGVLERQFRNYYIKASGRPGATGATLLQLLEGRLDNV
                     VYRMGFAATRNEARQLVSHAAIQVNGEKVTIASYQCKAGDVIAVREKSQKQLRIQTAL
                     QIKSQVGFPAWVEVDEKKFTGTFKQLPDREEILPDINENLVVELYSK"
     gene            complement(1035..1457)
                     /gene="rpsK"
                     /locus_tag="VEW08_07085"
     CDS             complement(1035..1457)
                     /gene="rpsK"
                     /locus_tag="VEW08_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HYJ40535.1"
                     /translation="MADQKQQQGQAGGGTQKPGRKKVKKHVTDAIAHVHASFNNTIVT
                     ITDRQGNTLSWATSGGCGFRGSRKSTPFAAQVAAEKAGVAAQEHGVKMVEVRVKGPGP
                     GRESAVRALNACGLKITNISDVTPIPHNGCRPPKKRRV"
     gene            complement(1476..1856)
                     /gene="rpsM"
                     /locus_tag="VEW08_07090"
     CDS             complement(1476..1856)
                     /gene="rpsM"
                     /locus_tag="VEW08_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012519216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HYJ40536.1"
                     /translation="MARIAGINIPMNKHVWVGLTSIFGVGRTQALKACTGANVKPNTI
                     VKDLTESEVAALRSQIGKLTVEGDLRREVSMNIKRLMDLGCYRGIRHRRGLPLRGQRT
                     RTNARTRKGPRKAAIKMNSPAGKA"
     gene            complement(1962..2078)
                     /gene="rpmJ"
                     /locus_tag="VEW08_07095"
     CDS             complement(1962..2078)
                     /gene="rpmJ"
                     /locus_tag="VEW08_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HYJ40537.1"
                     /translation="MKVRASVKKICKNCKIVRRKRVIYVICTSDARHKQRQG"
     gene            complement(2093..3388)
                     /gene="secY"
                     /locus_tag="VEW08_07100"
     CDS             complement(2093..3388)
                     /gene="secY"
                     /locus_tag="VEW08_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HYJ40538.1"
                     /translation="MTRFAELRQRLFFLLGALVVYRVGTFIPAPGIDPVALARFFQEQ
                     QGTILDIFNMFSGGALERLSIFALGVMPYISASIIIQMMAVVMPQLAAIKKEGESGRR
                     KLTQYTRYGTVLLATVQGASAAYAFQNQGVVINPGPQFIFSATITLIAGTMFLMWLGE
                     QITERGLGNGISMIILAGIVAGLPSAVGGTLEIVRTGEMNPAFALILFAAIIGVTAFV
                     VFVERGQRRIQVNYAKRQQGRRMYAGQSTHLPFKLNMSGVIPPIFASSLLLFPATIAS
                     WSGTSGGFSWLQNVSAAMTPGQPAHMILYGGLIIFFCFFYTALVFNSRETAENLKKSG
                     AFIPGIRPGQQTGEYIDKVLTRLTVWGALYVTTVCLLPEVLMSRFSVPFYFGGTSLLI
                     MVVVIMDFQAQVAAHLMSHQYEGLMKKANLRGYGRPGQS"
     gene            complement(3449..3892)
                     /gene="rplO"
                     /locus_tag="VEW08_07105"
     CDS             complement(3449..3892)
                     /gene="rplO"
                     /locus_tag="VEW08_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HYJ40539.1"
                     /translation="MRLNDMSPATGSKKIRLRVGRGASAGQGKTCGRGVKGQRARKGG
                     YHKVGFEGGQMPLQRRLPKVGFRSKIAPLVAEVRLSELAKIKGDLIDLASLKAANLIP
                     EQSLRARIVLSGTVARAVTVMSTNGVVHVTKGARSAIEGAGGKIE"
     gene            complement(3895..4080)
                     /gene="rpmD"
                     /locus_tag="VEW08_07110"
     CDS             complement(3895..4080)
                     /gene="rpmD"
                     /locus_tag="VEW08_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HYJ40540.1"
                     /translation="MANKQIKVTLVKSVFGQLKSHRATVRGLGLRRIRDTATVADTPE
                     VRGMIRASGHLLKVEEA"
     gene            complement(4098..>4189)
                     /gene="rpsE"
                     /locus_tag="VEW08_07115"
     CDS             complement(4098..>4189)
                     /gene="rpsE"
                     /locus_tag="VEW08_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319713.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HYJ40541.1"
                     /translation="RATMNALTSTQSPDEIAAKRGKRVEEILG"
BASE COUNT          751 a         1324 c         1305 g          809 t
ORIGIN      
        1 ttcttgtgga gacgcagctg cgtctcttcg cgcgcgttca gacggatcgc gacctgcttc
       61 aggttcagca gaatgtcgac gacgtcttcc tgcacgcctt cgatgctcgt gtactcgtgc
      121 aacacgccgt cgatctcagc ctcggtgatt gcacaaccgg gcatggacga cagcagcacg
      181 cgccggagcg cgttgccgag agtgtggcca aagccacgct cgaacggctc gatcacgacc
      241 ttcgcctgct tggcagtggt cggggtaact ttgacgacac gcggcttcag gaattcgcca
      301 acggatgcct gcatggagga ctcacctgct tggtagcggt taggactgcg agcgctcgcc
      361 tcgttacagc gataacgcct gcagtgaact aaagaaacga ctacttcgaa tacaactcga
      421 cgacgagatt ctcgttgatg tcgggcagaa tctcttcgcg gtcgggcagc tgcttgaacg
      481 tgccggtaaa tttcttctcg tcgacctcga cccacgccgg gaacccgacc tgggacttga
      541 tctgcagggc tgtctggatg cgcagctgct tctgcgactt ctcacgcacg gcgattacgt
      601 cgcccgcctt gcactgatac gaggcgatag tgaccttctc gccgttcacc tggatcgccg
      661 cgtggctcac cagctggcgc gcctcgttgc gcgtcgctgc gaagcccatg cgatagacca
      721 cgttgtcgag ccgtccctcg aggagctgca ggagcgttgc gcctgtagca ccgggccggc
      781 cagaagcctt tatgtagtag ttgcggaact gtctttcaag taccccgtac atgcggcgca
      841 gcttctgctt ctcgcgcagc tgcacgccgt agtcggaaag acgaccgcga cgctcgccct
      901 tgacgccgcc gggcggcact tcgagcttgc acttcgactc gagcggtttc acgccgctct
      961 tcaggaacag atccgttccc tcacgacgcg acagcttgca tttcggaccg agataacgag
     1021 ccatgaatct tcctctacac ccgccgcttc ttgggcgggc ggcagccgtt gtgcgggatc
     1081 ggcgtgacgt cggaaatgtt ggtgatcttc aagccgcagg cgttgagcgc gcgcaccgcg
     1141 gactcgcggc cggggcccgg gcccttgacg cgaacttcga ccatcttcac gccgtgttcc
     1201 tgcgcggcca cgccagcctt ctcagcggcg acctgcgcgg cgaacggcgt gctcttacgc
     1261 gagccgcgga aaccgcagcc gcccgacgtc gcccacgaca gcgtgttgcc ctggcggtcg
     1321 gtgatcgtga cgatcgtgtt gttgaaggac gcatgcacgt gcgcaattgc gtccgtgacg
     1381 tgcttcttga ccttcttgcg accgggcttc tgggtgccac cgcccgcctg gccctgctgt
     1441 tgcttctgat cagccatagt tcctctgcaa aacctttatg ccttaccggc cggcgaattc
     1501 atcttgatcg ccgccttgcg cgggcctttg cgtgtgcgtg cgttcgtgcg cgtgcgctga
     1561 ccacgcagcg gcaagccgcg acgatgacgg atgccgcggt agcaaccgag gtccatcagg
     1621 cgcttgatgt tcatcgacac ttcgcggcgc agatcgcctt cgaccgtcag cttgccgatc
     1681 tgcgagcgca gtgctgcgac ttcagactcg gtgaggtcct tcacgatggt gttcggcttc
     1741 acgttggcgc ccgtgcaagc cttgagcgcc tgcgtgcgac cgacgccaaa gatcgaggtg
     1801 agcccgaccc agacgtgctt gttcatcgga atgttgatgc cggctatacg agccattttg
     1861 gtgagctctc gtgcgcgtga agaaaagcgc ggaaatttat accaaaaacc ttagaaactc
     1921 aatgctttaa gagtcgggct aaagcccgac ctacgcaccc atcaaccctg ccgctgcttg
     1981 tggcgggcgt ccgaagtgca gatgacatag atgacccgct tgcggcggac gatcttgcag
     2041 ttcttgcaga tctttttgac tgaggcacga actttcatga caaaacaact ccttacgact
     2101 gccctggacg gccatagccg cgcaggttgg ccttcttcat caggccttcg tactggtgcg
     2161 acatcaggtg cgccgcgacc tgcgcctgga agtccatgat cacgacgacc atgatcagga
     2221 gcgaggtgcc gccgaaatag aagggcacgc tgaagcgcga catcagcact tccggcagca
     2281 gacacactgt agtgacgtac agggcgcccc agacggtcag gcgggtcagc accttgtcga
     2341 tgtactcgcc cgtctgctgg cctgggcgga tgcccgggat gaaggcgccg gacttcttca
     2401 ggttctcggc cgtctcgcgc gagttgaaca ccagcgcggt atagaagaag cagaagaaga
     2461 tgatcaggcc gccgtacagg atcatgtgcg ccggctgacc gggggtcatg gccgccgaga
     2521 cgttctgcag ccagctgaat ccgcccgagg tgcccgacca gctggcaatc gtcgccggga
     2581 acagcagcag gctggaggcg aagatcggcg ggatcacgcc cgacatgttc agcttgaacg
     2641 gcaggtgcgt gctctggccc gcatacatgc ggcggccctg ctggcgcttc gcgtagttca
     2701 cctggatgcg acgctgaccg cgctcgacga acacgacgaa ggccgtcacg ccgatgatcg
     2761 cggcgaacag gatcagtgcg aacgccgggt tcatttcgcc ggtgcggacg atctccagcg
     2821 tgccgccgac agccgatggc agaccggcga cgatgccggc caggatgatc atcgagatgc
     2881 cgttgccaag gccgcgctcg gtgatctgct cgcccagcca catcaggaac atcgttcctg
     2941 cgatcagggt gatggtcgcg gagaagatga attgcgggcc cgggttgatc accacgccct
     3001 ggttctggaa cgcgtacgcc gccgaggcgc cctgcacggt cgccagcaac acggtgccgt
     3061 agcgcgtgta ctgggtcagc ttacggcggc ccgactcgcc ttctttcttg atcgcggcga
     3121 gctgcggcat gaccaccgcc atcatctgaa tgatgatcga cgccgagatg tacggcatga
     3181 cgccgagcgc gaagatcgac agacgctcca gcgcgccgcc cgagaacatg ttgaagatgt
     3241 ccaggatcgt gccctgctgc tcctggaaga agcgcgcgag ggcaaccggg tcgatgccag
     3301 gtgccggaat gaacgtgccg acgcgataga cgaccagcgc acccagcagg aaaaacaggc
     3361 gctgacgcag ctccgcaaag cgggtcatct ctccgaatgc ggagagcgaa gcagtgctgg
     3421 cggttttgct gctggtggcc actgaaactt actcgatctt cccgccggcg ccttcgatgg
     3481 cggagcgcgc acccttggtg acgtggacga ctccgttagt cgacatgacc gtcacggccc
     3541 gagccacggt gccggacaag acgatacgcg cgcgcaggga ctgctccgga atcaggttcg
     3601 cggctttcag ggatgcaagg tcgatgaggt cgcccttgat ttttgcaagc tccgacaggc
     3661 gcacttcagc aaccagcggt gcaattttcg agcgaaaacc gactttgggc aggcggcgct
     3721 gcagcggcat ctggccgcct tcgaagccga ccttgtgata gccgccttta cgcgcgcgct
     3781 ggcccttgac gccgcggccg caggtcttgc cctggccggc ggaggcgccg cgtccgacgc
     3841 gcagtcggat tttcttgctg cccgtcgcgg gcgacatgtc attcagacgc atggttacgc
     3901 ttcctcaacc ttcaacaggt gcccggacgc gcgaatcatg ccgcgcactt ccggcgtgtc
     3961 ggcgaccgtc gcggtgtcgc ggatgcggcg caggccgagc ccgcgcacgg tcgcgcggtg
     4021 cgacttcagc tgcccgaaaa cgctcttcac gagcgtcacc ttgatctgtt tattggccat
     4081 ggctcgatgc ttccggatca gcccaggatc tcttcgacgc gcttgccgcg cttcgcggcg
     4141 atctcgtcgg gcgactgcgt gctggtgagc gcgttcatcg tcgcgcgca
//
LOCUS       DASQJS010000169        12850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1347120, whole genome shotgun sequence.
ACCESSION   DASQJS010000169 DASQJS010000000
VERSION     DASQJS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12850
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347120"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="VEW08_07120"
     CDS             complement(<1..1375)
                     /locus_tag="VEW08_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYJ40542.1"
                     /translation="MPQFFIDRPVFAWVIAIIITLGGVFAIRELPAEAYPDVAPPQVS
                     INATYPGANADVVERTVIQVIEQQLTGIDNLMYFSSSSSNGGGSITLFFESGTNPDTA
                     AVQTQNRVTLAEPRLPRDVVLQGLQVAKVNSGFLMAVSLRSKDNSVPSDVLNNIVAAQ
                     VLDPISRIPGVGQTQQFGAEYSMRIWLNPDKLRSFRMSAADVLARVRGQNVQFATGAL
                     GEQPAVAGQQVVAPVSAEAQFSSKEEFENVILRTEPNGTSVRLKDVGRVELGLNTYSF
                     DVRLNDQPVAGFGVLLSPGANALDVARLVKERMDELAPSFPAGVEWFLPFDATLFINA
                     AIHEVVVTLCIAVALVFIVMLVFLQSFRATLIPTLVVPVALMGAFIGMSIFGFSINQL
                     TLFGMVLAIGIVVDDAIVVIESVERIMREEHLSPKEATRKAMTQITNPIIAISVVLAA
                     VFVPSALQ"
     gene            complement(1404..2609)
                     /locus_tag="VEW08_07125"
     CDS             complement(1404..2609)
                     /locus_tag="VEW08_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ40543.1"
                     /translation="MPSRVQLIFAAVAALFVAACSKQEAQQQPPPPEVGVIVVHSGPV
                     PLVREASGRLAPTRASDVRARVPGVLQKRIYKEGSMVKEGDPLMIIDTAPYRAALAQA
                     TAALEQAEASATNAKVTADRNRELMKQNLVSRMQLDDSAALERSTAAQVSAARAQVQT
                     ARINLGYATITAPISGRAGQMRVLEGALVGQGEATLLTTVEQVDPIFVFFDQPASDFE
                     RLMRAQASGDVTLAQGNLAEVRLLRDDGTLYEEKGTLDFSDYSVNPTTGSVAFRGRVA
                     NPGRVLLPGMYVKVRLSVGTLNKGFRVPQLAVQRDGQGPFVLVAGGDGMVAQKRIVTA
                     SAVGGDWVISSGLADGDQVIVDNLQKIGPGMPVKAKPAQAEGVEAPATPAAAPQEKPA
                     EGSAAATAS"
     gene            complement(2670..3434)
                     /locus_tag="VEW08_07130"
     CDS             complement(2670..3434)
                     /locus_tag="VEW08_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HYJ40544.1"
                     /translation="MSRPRITRTRTHISTAEPNPLPGGADAQASLQLLRLLHLASPAL
                     PIGAFHFSQGLEYAVEAGWVTDEATAHEWIGGIAGASLGTLDLPVLLRLQAAWKQGDH
                     GGVLRWNSFLIACRETEELRAEDRHLGAALLRVLVEMDLTTGLFSANAAKSVVGVSHA
                     TAFAFACARWNIEPRASLQTFAWAWIENQVLAAVKLVPLGQSAAQRMLHRLVARIPPL
                     VEQAHKLTDSDVGISTASNAVASGRHETQYSRLFRS"
     gene            complement(3385..3858)
                     /gene="ureE"
                     /locus_tag="VEW08_07135"
     CDS             complement(3385..3858)
                     /gene="ureE"
                     /locus_tag="VEW08_07135"
                     /inference="COORDINATES: protein motif:HMM:NF009751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HYJ40545.1"
                     /translation="MLKINLKLDRATDHPRVADFKLVLPFGERSKSRLRAVLDNGEEA
                     GLFLERGSVLRHGDLLLADDGRVVEVQAAPETVSTVRTEDSLMLARACYHLGNRHVAL
                     QIGTAWLRYQHDHVLDDMLRGFGLEVVIEQLPFEPEGGAYVATSHHTHPHAHQHG"
     gene            complement(3873..5576)
                     /gene="ureC"
                     /locus_tag="VEW08_07140"
     CDS             complement(3873..5576)
                     /gene="ureC"
                     /locus_tag="VEW08_07140"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HYJ40546.1"
                     /translation="MTRIDRRAYAEMYGPTTGDRVRLGDTELIIEVERDHTIYGEEVK
                     FGGGKVIRDGMGQGQRSAKEVADTVITNALIVDHWGIVKADIGLKEGRIAGIGKAGNP
                     DVQPGVDIIIGAGTEIIAGEGLIATAGGIDSHIHFICPQQVDDAMMSGITTMIGGGTG
                     PAAGTSATTCTPGPWYIGRMYQALDELPINFGLLGKGNASLPAPLDEQIEAGVMGLKL
                     HEDWGTTPAAIDNCLNVAEKYDIQVAIHTDTLNESGFLEDTLAAFKGRAIHTYHTEGA
                     GGGHAPDIIKACGESNVLPSSTNPTRPYTVNTVDEHLDMLMVCHHLDPGIAEDVAFAE
                     SRIRRETIAAEDILHDKGAMSMISSDSQAMGRIGEVITRTWQTAHKMKVQRGPLKPDS
                     ARNDNFRVRRYIAKYTINPAITHGIAHEVGSVEKGKLADIVLWKPAFFGAKTAMIIKG
                     GMIVAAPMGDPNASIPTPQPVHYRPMFGALAGARHATCVHFVSKAGVANGMAGNLDLK
                     KRLVAVQNTRAIRMKDLILNDYQPKIEVDSQTYEVRADGEALRCEPAAVLPLAQRYFL
                     F"
     gene            complement(5573..5881)
                     /locus_tag="VEW08_07145"
     CDS             complement(5573..5881)
                     /locus_tag="VEW08_07145"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004644206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HYJ40547.1"
                     /translation="MNPGEIFCENGDIEINAGRATRKVKVANTGDRPIQVGSHYHFFE
                     TNRALRFERAPTKGFRLNIAAGTAVRFEPGQEREVTLVAYGGAREVFGFDGQIMGKLP
                     "
     gene            complement(5889..6191)
                     /gene="ureA"
                     /locus_tag="VEW08_07150"
     CDS             complement(5889..6191)
                     /gene="ureA"
                     /locus_tag="VEW08_07150"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008054002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HYJ40548.1"
                     /translation="MELTPREKDKLLIFTAGLVAERRKARGLKLNYPEAVALISAAIL
                     EGARDGRTVAELMSFGTTILSRADVMDGVPEMIPEVQVEATFPDGTKLVTVHHPIP"
     gene            complement(6202..7104)
                     /locus_tag="VEW08_07155"
     CDS             complement(6202..7104)
                     /locus_tag="VEW08_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HYJ40549.1"
                     /translation="MSAAPGDPVLPGWQAELRLRFNGDAPQASTRTRLVERQHKGPLI
                     VQRPFYPEGDPCHVYLVHPPGGVVGGDELRIDVQVDAGAHALITTPAATKFYRCDGRI
                     SSQTQELRAAGATLEWLPQENIFYRGADARTATRVHIDADSRFIGWEINCLGLPARGE
                     SFDAGALRLDLELWRSLPALEPAPIKPDPFCRDKGAAPIFLDRVRLTGESPSRTARWG
                     LAGQEAIGTLLATPATREAVESVRELIADAPYAAVSLVDGVLVLRALAPQAEAVRNLF
                     ISAWQSLRPRILGRGAVLPRIWAT"
     gene            complement(7107..7817)
                     /gene="urtE"
                     /locus_tag="VEW08_07160"
     CDS             complement(7107..7817)
                     /gene="urtE"
                     /locus_tag="VEW08_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HYJ40550.1"
                     /translation="MLAIHGLNQFYSGSHILWDVNLEVPEGSCTCLMGRNGMGKTTLL
                     KCIMGLLPIASGGMSYGFGAETADLTKVSADRRARLGIAYVPQGREIFSQLTVEENLR
                     IGFGARRGPAINDIPEHIFEWFPVLRKMLRRRGGDLSGGQQQQLAIGRAMVLDPKLLI
                     LDEPAEGIQPNIVAEIGDIIMRLNREAKLTVLLVEQKLPFARRVASEFRIIDKGRIVN
                     GGEIGALTDNLVHQHLTV"
     gene            complement(7820..8713)
                     /gene="urtD"
                     /locus_tag="VEW08_07165"
     CDS             complement(7820..8713)
                     /gene="urtD"
                     /locus_tag="VEW08_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HYJ40551.1"
                     /translation="MNAAQVVKQGWQKVFGAGAGSDRPMPGDASHGTILYVEDITVSF
                     DGFRALNNLTLYIDVGELRCIIGPNGAGKTTMMDVITGKTRPDAGSAWFGTTTDLLTL
                     TEPEIAEAGIGRKFQKPTVFEQHSVLQNLALAAAGNKSVWHTLFKPIARAQFARIDEV
                     LVTVGLKEERNSRAGILSHGQKQWLEIGMLLMQDPRLLLVDEPVAGMTPQEVERTAEL
                     LLSLAGKHSVVVVEHDMEFVRSIARTVTVLHEGSVLAEGSMDAVQNDPRVVEVYLGEP
                     QEPGKPRKPASKAHSAPMGSS"
     gene            complement(8710..9858)
                     /gene="urtC"
                     /locus_tag="VEW08_07170"
     CDS             complement(8710..9858)
                     /gene="urtC"
                     /locus_tag="VEW08_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HYJ40552.1"
                     /translation="MKRGPILNLLVGERGTAVFLAIVAIVGIVVPVMHLLPDEGSVLQ
                     LSGYTMTLIGKYMTYALLAVAVDLVWGYCGILSLGHAAFFALGGYAMGMYLMRQIGTR
                     GVYGDPVLPDFMVFLNYKELPWFWHGFDMFWFAMLMVAVVPGLLALLFGWLAFRSRVT
                     GVYLSIITQALTYALMLAFFRNDMGFGGNNGMTDFKDILGFAINTPATRVALFIASAI
                     ALILGYLACRYITMSRAGRVVQAIRDAESRTRFIGYRVESYKLWIFTFSAVLAGIAGA
                     LYVPQVGIINPSEFQPINSIEVVIWVAVGGRGMLYGAAAGAVIVNYAKTFFTAALPEF
                     WLYALGLLFVVVTLFLPRGFVGLLDRFGGKPPAAPPSTTSETARAATA"
     gene            complement(9860..11509)
                     /gene="urtB"
                     /locus_tag="VEW08_07175"
     CDS             complement(9860..11509)
                     /gene="urtB"
                     /locus_tag="VEW08_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HYJ40553.1"
                     /translation="MLLRRFGLVAAFVLASVLFAVTATAQTTAPTAGDTFAAAVAGLA
                     AESFPEKSAAVTALSELKHPNGRAVLAALLDGKLYFRVADNKVYITDGGETALSLTDP
                     LTLAAAGSGEPDDFSRITTNNSLRKTLRSAVATFALSDPDADVRLAAVREMLRSIDDD
                     SVAILRTSMEKEADADVRKAMREGLALADLDSADADTRLTAVAALKDSLSPDVYNRLT
                     IMVTADDAGSFPEPDERVRTTAREVISAIDSSRQVYATLETLFFGLSLGSVLVLAAIG
                     LAITFGVMGVINMAHGELMMLGAYTTYVVQQAMPNHIGLSIFIAIPLAFLVSACVGML
                     IERGVVRFLYGRPLETLLATFGVSLILQQVVRDVFSANNRPVETPAWMAGSMQINEAL
                     SITYNRLYVILFMIVVFVVLQMVLQKTSLGLKVRAVAQNRNMARAMGVRTQWVDAMTF
                     GLGSGIAGVAGVALSQLTNVGPNLGQQYIIDSFMVVVFGGVGNLWGTLIGGMSLGVVS
                     KVLEPWAGAVLAKILVLIALILFIQRRPRGLFPQKGRAAEN"
     gene            complement(11579..12817)
                     /gene="urtA"
                     /locus_tag="VEW08_07180"
     CDS             complement(11579..12817)
                     /gene="urtA"
                     /locus_tag="VEW08_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HYJ40554.1"
                     /translation="MTMASAAQAADTIKVGVLHSLSGTIAISETTLKDTVLMLIDEQN
                     KKGGLLGKKLEAVVVDPASNWPLFAEKAEQLLVKDKVAVVFGCWTSVSRKSVLPVFEK
                     NNGLLFYPVQYEGEESSKNVFYTGAAPNQQAIPAVDYLMKDLGVKRWVLAGTDYVYPR
                     TTNKILEAYLQAKGVAPADIMINYTPFGHSDWQSIVADIKKFGSAGKKTAVVSTINGD
                     ANVPFYKELGNQKVSAEDIPVVAFSVGEEELAGIDTKPLVGHLAAWNYFMSVKSPVNS
                     EWIKKWVAYTKNPKRTFNDPMEAHVIGFQMWVKAVEKAKTTDVTKVTDAMIGLEAPNL
                     TGGIAKMLPNHHLTKPVLIGEVRADGQFDTVWKTTGLVPGDAWSDFLPGSKDIEADWV
                     TLKCGNYNTKTKQCSGQNYK"
BASE COUNT         2358 a         4050 c         4057 g         2385 t
ORIGIN      
        1 attgcagcgc gctggggacg aacaccgccg cgagcaccac cgagatcgcg atgatggggt
       61 tggtgatctg cgtcatcgcc ttgcgcgtag cttccttggg cgacaagtgc tcctcgcgca
      121 tgatgcgttc gacggattca atgaccacga tggcatcgtc gaccacgatg ccgatggcca
      181 gcaccatgcc aaacaaggtc agctggttga tcgagaaacc gaagatcgac atgccgatga
      241 atgcgcccat cagcgccacc ggcaccacca gcgtgggaat gagggtggcg cggaagctct
      301 gcaggaacac cagcatgacg atgaacacca gcgccacggc gatgcagagc gtgacgacca
      361 cctcgtgaat cgcggcgttg atgaacagcg tcgcgtcgaa aggcaggaac cactccacgc
      421 cggccgggaa gctgggcgcg agttcgtcca tgcgctcctt gaccagcctg gcgacatcga
      481 gcgcattcgc accgggtgac agaagaacgc cgaaaccggc gacgggctgg tcgttcaatc
      541 gtacgtcgaa cgaataggtg ttgagaccga gctcgacgcg cccgacgtcc ttcagccgca
      601 cgctggtgcc gttcggctcg gtgcgcagga tgacgttttc gaattcctct ttggaactga
      661 actgcgcttc cgccgacacg ggcgccacca cctgctgtcc cgcgacggca ggctgttcac
      721 ccagcgcgcc ggtcgcgaac tgcacgttct ggccacgcac gcgggcgaga acatcggcgg
      781 cactcatgcg gaagctgcgc agcttgtccg gattcagcca gatgcgcatc gagtattcgg
      841 cgccgaactg ctgcgtctgt cccactcccg ggatacgcga gatcgggtcg agcacctggg
      901 cggcgacgat gttgttgagc acgtccgaag gcacgctgtt gtccttcgaa cgcagactca
      961 cggccatgag gaagcccgag ttcaccttcg ccacctgcag gccctgcaac accacgtcgc
     1021 gcggcaggcg cggctcggcc agtgtcacgc ggttctgggt ctgcaccgcc gcggtgtcgg
     1081 gattcgtgcc ggactcgaag aagagcgtga tgctgccgcc gccattggag gacgacgagg
     1141 agaagtacat caggttgtcg atgccggtga gctgctgttc gatgacctgg atgaccgtgc
     1201 gctcgaccac gtcggcgttg gcgccagggt aagtcgcgtt gatggagacc tgtggcggcg
     1261 cgacgtccgg gtacgcttcc gccggcagct cgcgaatggc gaacacacca ccgagggtga
     1321 tgatgatggc gatcacccat gcgaataccg ggcgatcgat gaaaaattgc ggcattggag
     1381 tcagccctgc gagtagtgtg gcgctaacta gcggtggctg cagcggagcc ttcggcaggc
     1441 ttctcttgcg gagccgccgc tggagtggcc ggagcttcaa cgccctccgc ctgtgccggc
     1501 ttcgccttca caggcatgcc cggcccgatc ttctgcagat tgtcgacgat gacctggtcg
     1561 ccgtcggcga gaccgctgga gatcacccag tcgccgccaa cggccgaggc agtcacgatg
     1621 cgcttctgcg ccaccatgcc gtcgccgccg gcgaccagca cgaacggacc ctggccgtca
     1681 cgctgcaccg cgagttgcgg cacgcggaac cccttgttca aggttccgac ggagagccgc
     1741 accttcacgt acatgcccgg cagcagcacg cggccgggat tcgccaccct gccccggaac
     1801 gccacagagc cggtagtcgg gttcacgctg tagtcggaga aatcgagcgt gcccttctcc
     1861 tcgtaaagcg tgccgtcgtc gcgtaacagc cgcacctcgg cgaggttgcc ctgggcaagc
     1921 gtcacgtcac ccgatgcttg cgcgcgcatc aggcgctcga agtcggatgc cggctggtcg
     1981 aagaacacga agatgggatc gacctgttcg acggtagtta gcagggtcgc ctcgccctgc
     2041 ccgaccagcg cgccttccag cacacgcatc tgcccggcgc ggccggagat gggcgcggtg
     2101 atggtggcgt agccaaggtt gatgcgcgca gtctgaacct gcgcgcgggc cgcggacacc
     2161 tgcgcggcgg tcgagcgttc gagcgccgcg gaatcgtcga gctgcatgcg cgacaccagg
     2221 ttctgcttca tgagctcccg gttgcgatcg gcggtcacct tcgcgttggt cgcggaggct
     2281 tcggcttgtt cgagcgcggc ggtcgcttgc gcgagcgcgg cgcgatacgg cgccgtgtcg
     2341 atgatcatga gggggtcgcc ctctttgacc atgctgccct ccttatatat gcgcttctgg
     2401 agcacacctg ggacacgggc gcgaacgtcc gatgcgcgcg tgggcgcaag tcgcccggag
     2461 gcttcgcgca ccagcggtac cggcccggaa tgcacgacga tgacgccgac ttccgggggt
     2521 ggtggttgct gctgtgcctc ctgctttgag caggcggcga caaacaaagc ggcaacagcc
     2581 gcgaatatca gctgaacgcg tgaaggcatt ttgtttttag gcacggcggg ggttggaatg
     2641 tccgcctgcg gtggcggaga ttctagccct tacgaacgga acagtcgcga gtactgagtt
     2701 tcatgtcggc cactggccac ggcgttagag gcggtggaga ttccgacgtc ggaatctgtg
     2761 agtttgtgtg cctgctctac caaaggaggg atgcgcgcga cgagccgatg cagcattctt
     2821 tgcgcggcgc tttgtcccag tggaaccagc ttcacggccg ccaacacctg gttctctatc
     2881 cacgcccagg caaaggtctg caaggacgcc cggggctcga tattccaccg cgcgcacgcg
     2941 aatgcgaacg cagtcgcgtg tgacacaccg acgacgctct ttgcggcatt cgcggaaaac
     3001 agcccggtcg tcaggtccat ctcgacgagc acacgcagca gtgcggcgcc caggtgccga
     3061 tcctcggcgc gcaactcttc ggtttcacgg caggcgatga gaaaggaatt ccatcgcagg
     3121 acgccaccgt ggtcaccctg tttccacgcg gcttgcagac gcagcagcac cggcagatcg
     3181 agtgtgccca ggcttgcccc ggcaatgccg cctatccact cgtgcgccgt cgcttcgtcg
     3241 gtcacccatc cggcttcgac cgcgtactcc agaccttgcg aaaaatgaaa cgcaccgatg
     3301 ggcagcgcgg ggctcgccag gtgcaacagg cgcagcaact gcagcgatgc ctgcgcatcc
     3361 gcacctccgg gcagcggatt cggttcagcc gtgctgatgt gcgtgcgggt gcgtgtgatg
     3421 cgaggtcgcg acatacgcgc ctccttcggg ctcgaacggc aactgttcga tgacgacctc
     3481 gagtccgaat ccgcgaagca tgtcgtcgag cacgtgatcg tgctggtagc gaagccacgc
     3541 ggtgccgatc tgcagtgcca cgtgtcggtt gccgaggtga taacaggcgc gcgccagcat
     3601 caggctgtct tcggtgcgga ccgtcgacac ggtttcgggc gcggcctgca cttcgaccac
     3661 gcgaccatca tcggcgagca gcaagtcgcc atggcgcagc actgagcctc gttcgagaaa
     3721 cagacctgct tcctccccgt tgtcgagcac cgcgcgaagc cggctcttcg agcgctcacc
     3781 gaaaggcagc accagcttga aatcggcgac acgagggtga tcggtggcgc gatcgagctt
     3841 gaggtttatc ttgagcatcg gaacagacca aatcagaaca ggaagtatcg ctgcgcaagc
     3901 ggcagcacag cagccggttc gcagcgcagc gcctcgccgt ccgctcgcac ttcgtaagtc
     3961 tgcgaatcca cttcgatctt cggttgatag tcgttgagga tgagatcctt catgcgaatg
     4021 gcacgcgtgt tctgcacggc cacgagccgc ttcttcaggt ccaggttccc ggccatgcca
     4081 ttggcgacac cggccttgga cacgaaatgc acgcaggtcg catgccgcgc gcccgcgagc
     4141 gctccgaaca tgggacggta gtgcacgggc tgcggcgtgg ggatggatgc attgggatca
     4201 cccatgggtg cggcgacgat catgccgccc ttgatgatca ttgcggtctt ggcgccgaag
     4261 aacgccggct tccacaacac gatgtcggcg agcttgccct tctcgaccga accgacttca
     4321 tgcgcgatgc cgtgggtgat tgccgggttg atcgtgtact tggcgatgta gcgccgtact
     4381 cggaagttgt cgttgcgcgc gctgtcgggc ttcaatggcc cgcgctgcac cttcatcttg
     4441 tgcgcggtct gccaagtacg cgtgatgact tcaccgatgc ggcccatggc ctgcgaatcc
     4501 gaggagatca tcgacatcgc gcccttgtcg tgcaggatgt cttcggcggc gatggtctcg
     4561 cggcgaatgc gcgactcggc aaacgccacg tcctcggcga ttccgggatc gaggtggtga
     4621 cacaccatga gcatgtcgag atgttcatcg acggtgttca cggtgtacgg acgagtggga
     4681 ttcgtggacg acggcaacac gttcgattcg ccgcaagcct tgatgatgtc gggtgcgtgc
     4741 ccgccgccgg cgccttcggt gtgataggtg tgaatcgcgc ggcccttgaa tgcggcgagc
     4801 gtgtcttcga gaaatccgga ttcgttgagc gtgtcggtat ggatagccac ctggatgtcg
     4861 tacttctcag cgacgttcag gcagttgtcg atggcggcgg gcgtggtgcc ccagtcttcg
     4921 tgcagcttga gccccatgac accggcttct atctgctcgt cgagtggtgc gggcaggctc
     4981 gcattgcctt tgcctaataa accgaaattg atgggcagct cgtccagtgc ctggtacatg
     5041 cgcccgatat accagggccc gggtgtacag gtggtggcgc tggtgcccgc cgcggggcca
     5101 gtgccaccac cgatcatggt cgtgatgccc gacatcatcg cgtcgtccac ctgctgcgga
     5161 cagatgaagt ggatgtgcga gtcgatgccg ccggcggtgg cgatgagccc ctcgcccgcg
     5221 atgatctccg tgcccgcgcc gatgatgatg tccacgccgg gctggacgtc cgggttgcca
     5281 gccttgccga tgcccgcgat gcgcccttcc ttcagtccga tgtcggcctt gacgatgccc
     5341 cagtgatcga cgatgagcgc gttggtgatg accgtgtcgg caacttcctt cgcgctgcgc
     5401 tgaccctggc ccatgccatc gcgaatcacc ttgccgccgc cgaatttcac ttcctcgccg
     5461 tagatggtgt ggtcgcgctc gacttcgatg atgagctcgg tgtcgccgag ccgaacgcga
     5521 tccccagtgg tggggccgta catctccgca taggcgcggc ggtcaattcg agtcacggca
     5581 gtttccccat gatctgtcca tcgaagccga acacctcgcg cgcaccgccg taggccacca
     5641 gcgtgacctc gcgttcctgg cctggctcga agcgaacggc ggtgccggcg gcgatgttga
     5701 gacggaatcc cttcgtgggc gcgcgttcaa accgcaaagc cctgttggtc tcgaagaagt
     5761 ggtagtgcga gccgacctgg atcggccggt cgccggtgtt ggcgaccttc accttgcgcg
     5821 tagcccggcc cgcgttgatt tcgatgtcac cgttttcgca gaaaatttcc ccggggttca
     5881 tggtgctctc acggaatggg atggtgaacg gtgacgagct tggtgccgtc tgggaatgtg
     5941 gcctcgacct gcacctcggg gatcatctcg ggcacgccat ccatgacgtc ggcgcggctc
     6001 agtatggtag tgccgaagct catgagctcg gcgacggtgc ggccatcgcg tgcgccttcg
     6061 agaatggccg cggagatcaa cgccaccgct tccggatagt tgagcttcag cccgcgcgcc
     6121 ttgcggcgct cggcgaccag gccagccgtg aagatgagta gtttgtcttt ctcgcgcggt
     6181 gtcagttcca tgtcgttcga ctcacgtggc ccagatgcgc ggcagcacgg ccccacgccc
     6241 gaggatgcgg gggcgcaggc tttgccacgc ggagatgaaa agattgcgca ccgcctccgc
     6301 ctgcggcgcc agcgcacgca gtaccagcac accgtccacg agcgaaaccg cggcataggg
     6361 tgcatcggcg atgagttcgc gaacggattc cacggcctcg cgagttgccg gcgtcgccaa
     6421 cagggttcca atcgcttcct ggcccgcgag accccagcgc gcggtgcgcg aggggctctc
     6481 gcccgtgagc ctgaccctgt cgagaaatat gggggcagcc cccttgtccc ggcaaaaggg
     6541 gtcaggcttg ataggcgcag gctcaagcgc tggaagcgat cgccacaact ccagatcgag
     6601 tcgcaacgcg cccgcatcaa acgattcgcc gcgcgcggga agacccaggc agttgatctc
     6661 ccagccgatg aagcgggaat ccgcgtcgat gtgcacgcga gtcgcggtgc gcgcatcggc
     6721 cccgcggtag aagatgttct cctgcggcaa ccattcgaga gtagcgccag cggcgcgcaa
     6781 ctcctgggtc tgcgatgaaa tgcggccatc gcagcgatag aacttcgtgg cagcgggtgt
     6841 cgtgatgagt gcgtgggcac ccgcatctac ctggacatcg atgcgcagct catcgccgcc
     6901 cactacgcct ccgggtggat gcacgagata gacgtggcag ggatctccct cgggatagaa
     6961 cggccgctgg acgatgagcg gacctttgtg ttgacgctcg acgaggcgcg tgcgcgtgct
     7021 cgcttgaggc gcgtctccat tgaatcgcag gcgaagctcg gcctgccaac cgggaaggac
     7081 gggatcaccg ggtgcagcgc tcatcgtcac acggtgagat gctggtggac gagattgtca
     7141 gtcaatgcgc cgatctcacc gccgttcaca atgcggcctt tgtcgatgat gcggaactcg
     7201 ctggccacgc ggcgcgcaaa cggcagtttc tgttccacta acagcacggt gagtttcgcc
     7261 tcgcggttga ggcgcatgat gatatcgccg atctcggcga cgatgttggg ctgaatgccc
     7321 tcggccggct catcgagaat cagcagcttc ggatcgagca ccatcgcgcg gccgatcgca
     7381 agctgctgct gctgcccgcc tgacaagtct cctccccttc gccgcagcat tttcctgagc
     7441 acgggaaacc attcgaaaat gtgttcggga atgtcgttga tcgcgggtcc gcgccgcgcc
     7501 ccgaagccaa tgcgcaggtt ttcctcgacc gtgagctgtg aaaagatttc gcggccctgg
     7561 ggcacatagg caatgcccag ccgcgcccga cgatccgcgg acactttcgt caggtctgca
     7621 gtttcggcgc cgaatccgta actcatgccg cccgaagcaa tcggcagcag ccccatgatg
     7681 cacttgagca gcgtggtctt gcccatccca ttgcgtccca tgaggcatgt gcacgagcct
     7741 tcgggcacct cgagattcac atcccacagg atgtgactgc cgctgtagaa ctggttgagc
     7801 ccgtggatgg ccagcatcgc tagctacttc ccatgggagc cgagtgcgct ttcgatgccg
     7861 gtttccgcgg cttcccgggc tcctgaggct cgccgagata gacctccacg accctcggat
     7921 cattctgcac ggcatccatg ctgccttcgg cgagtacgga gccttcgtgc agcaccgtca
     7981 cggtgcgcgc gattgagcgc acgaactcca tgtcgtgctc cacgacaacc acggaatgtt
     8041 tgccggcgag cgagagcagg agctcggccg tgcgttcgac ttcctgcggc gtcatgcctg
     8101 ccaccggctc gtcgaccagc agcagccgcg gatcctgcat cagcaacatg ccgatctcga
     8161 gccactgctt ctggccgtgc gagagaattc cggcgcgtga attgcgttcc tctttcagtc
     8221 cgaccgtgac cagcacttca tcgatgcgcg cgaactgcgc tcgcgcaatc ggcttgaaca
     8281 atgtgtgcca gaccgacttg tttcccgcgg ccgcgagcgc caggttctgc aggacactgt
     8341 gctgttcgaa taccgtcggc ttctgaaatt tccgtccaat tccggcctcc gcaatttcag
     8401 gttcggtgag cgtgagaagg tctgtcgtcg taccaaacca ggcggagcct gcgtccgggc
     8461 gcgttttgcc ggtgatcacg tccatcatcg tcgtcttgcc ggcaccgttt ggtccgatga
     8521 tgcagcgaag ctcgccgaca tcgatgtaca gcgtgagatt gttgagcgca cgaaagccat
     8581 cgaaactcac cgtgatgtct tcgacataca ggatcgtgcc gtgcgaagca tcgcccggca
     8641 tggggcggtc gctgccggca ccggcgccga atactttctg ccagccctgt ttgaccacct
     8701 gcgcggcgtt catgcggtcg ccgccctggc agtttcgctc gtcgtggaag gcggcgcagc
     8761 cggcggcttg ccgccgaagc ggtccaacaa gcccacgaat cctcgcggca ggaacagcgt
     8821 gaccaccacg aacagaaggc cgagcgcgta taaccagaac tccggcagcg cggcggtgaa
     8881 gaaggtcttc gcatagttga cgatgacggc gccggccgcg gcgccgtaca gcatgcctcg
     8941 ccctcccacg gccacccaga tcaccacttc tatggagttg atgggctgga actcgctggg
     9001 attgatgatg ccgacctgcg gcacgtaaag cgcaccggca atgccggcga gcaccgccga
     9061 gaaagtgaaa atccagagct tgtacgactc gacgcggtag ccgataaagc gcgtgcgact
     9121 ctctgcatcg cgaatcgcct gcaccacgcg tccggcgcgc gacatcgtga tgtagcggca
     9181 cgcgagatac ccgaggatga gcgcaatggc gctggcaatg aagagcgcca cgcgcgttgc
     9241 cggagtattg atggcaaacc ccaggatatc cttgaagtcc gtcatgccgt tgttgccgcc
     9301 gaagcccatg tcattgcgga aaaacgccag catcaacgcg tatgtgagcg cctgcgtgat
     9361 gatggataga tagacaccgg tgacgcgtga gcggaacgcc agccagccga acagcagcgc
     9421 cagcaacccg ggcaccaccg ccaccatcag catggcgaac cagaacatgt cgaagccgtg
     9481 ccagaaccac ggcagctcct tgtagttcag gaacaccatg aaatccggca gcaccggatc
     9541 cccgtacacc ccacgcgtgc cgatctgccg catgaggtac atgcccatgg cgtaaccgcc
     9601 gagcgcgaag aacgccgcat ggccgagcga caggatgccg cagtagcccc acacgagatc
     9661 cacggccacg gccagcaacg cgtacgtcat gtacttgccg atgagcgtca tggtgtaacc
     9721 cgacagttgc agcacggagc cttcgtccgg caacaggtgc atgaccggaa cgacgatgcc
     9781 gacgatcgcg acgatggcga gaaacacggc cgtgccgcgt tcaccgacca gcaggttgag
     9841 aatgggaccc cgtttcatcc tagttctccg ccgcccggcc cttctgcggg aataacccgc
     9901 gcggccggcg ctggatgaac aggatgagcg cgatcaacac caggattttg gccagtaccg
     9961 caccagccca gggctcgagc accttgctga ccacgcctaa cgacatgcct ccgatcaatg
    10021 tgccccacag atttccgacg ccgccgaaca ccaccaccat gaaggaatcg atgatgtatt
    10081 gctgacccag gttgggaccg acgttggtga gctggctgag cgcgacgccg gctacacccg
    10141 cgatcccgga ccccagaccg aaggtcatcg catccaccca ttgcgtgcga acgcccatgg
    10201 cacgtgccat gttgcggttc tgtgccaccg cgcggacctt gaggcccagc gaggttttct
    10261 gcagcaccat ctgcaggact acgaacacca cgatcatgaa caggatgacg tacaggcggt
    10321 tgtaggtgat cgacagtgct tcgttgatct gcatcgagcc cgccatccag gccggcgtct
    10381 ccaccggacg attgttggca ctgaaaacgt cacgcaccac ctgttgaagg atgaggctga
    10441 cgccgaacgt ggctaacaag gtttcgagcg gccggccgta caggaaacgc accacgccgc
    10501 gttcgatgag catgccgacg caggcggaca ccaggaatgc gagcggaatt gcgatgaaga
    10561 tcgacaggcc gatgtgattg ggcatcgcct gctgcaccac gtaggtggtg taggcaccga
    10621 gcatcatgag ctcgccatgc gccatgttga tgacgcccat gacgccaaac gtgattgcga
    10681 gcccgatggc ggcgagcacc agcacggaac cgagactcag gccgaagaac agggtttcga
    10741 gcgtcgcgta gacctggcgc gaggaatcga tcgccgaaat cacctcgcga gccgtggtgc
    10801 gcacgcgctc atcgggttca ggaaaggaac ccgcatcgtc ggctgtcacc atgatcgtca
    10861 gccggttgta aacatcgggg ctcagcgagt ctttcagggc agccacggcg gtgagccgcg
    10921 tgtccgcatc cgcgctgtcg agatcggcga gcgcgagacc ctcgcgcatt gccttcctga
    10981 cgtcagcgtc ggcctccttc tccatgctgg tccgcaggat ggccacgctg tcgtcgtcga
    11041 tcgagcgcag catttcgcgc accgcggcga gccgcacgtc ggcatccgga tccgaaagcg
    11101 cgaaggttgc gacggcactg cgaagtgtct tgcgcaggct gttgttggtc gtgatgcgcg
    11161 agaagtcgtc cggctcgccg ctgccggccg ccgcgagcgt caacggatcg gtcagcgaga
    11221 gcgctgtctc accgccgtcg gtgatgtaaa ccttgttatc cgcaacgcgg aaatagagct
    11281 tgccgtcgag cagcgccgcg agaacggcgc ggccgttggg gtgtttgagt tcactcagcg
    11341 cggttaccgc cgcggacttc tccggaaagc tttccgccgc gagtccggcc acggccgcgg
    11401 cgaatgtatc gcccgccgtc ggagcggtag tttgagctgt cgcggtcacg gcgaacaaca
    11461 cgctggccag cacgaaggca gcgaccaggc cgaagcggcg cagaagcaaa agagaaggtc
    11521 ccgggattgc tcccgggacc tcaattgcct tttcaacgga ctttttcaga agttcttgtt
    11581 atttgtagtt ctggcccgag cactgcttgg tcttggtgtt gtagttgccg cacttcaacg
    11641 tgacccagtc agcctcgatg tccttgctgc ccggaaggaa gtcggaccat gcgtcgcccg
    11701 gaacgagtcc cgtggtcttc cacaccgtgt cgaactggcc atcggcacgc acttcgccga
    11761 tcagcacggg cttggtcagg tgatggttag gcagcatctt ggcgattcca ccggtgaggt
    11821 tcggtgcttc gagaccgatc atcgcgtcgg tgactttcgt cacgtccgtg gtcttggcct
    11881 tctccacggc cttcacccac atctggaagc cgatcacgtg cgcttccatc ggatcgttaa
    11941 aggtgcgctt cgggttcttg gtgtacgcga cccacttctt gatccactcc gaattcacgg
    12001 gactcttgac gctcatgaag tagttccacg cggcgagatg gcccaccagc ggcttggtgt
    12061 cgatgcccgc gagttcctct tcacccaccg agaaagccac aaccggaatg tcttccgccg
    12121 acactttctg attgccgagt tccttgtaga acggcacgtt ggcgtcaccg ttgatggtcg
    12181 aaaccaccgc ggtctttttg ccagccgagc cgaacttctt gatgtcggcc acgatcgact
    12241 gccagtcgga gtgaccgaaa ggcgtgtaat tgatcatgat gtcggcgggc gcgacgccct
    12301 tggcttgcag gtaggcctcg aggatcttgt tggtggtgcg cggataaacg tagtccgtac
    12361 cagcgagcac ccagcgtttc acgccgagat ccttcatcag gtagtcgacg gccgggatag
    12421 cctgctggtt cggcgccgcg ccggtgtaga acacgttctt cgacgattct tcaccttcgt
    12481 actgcaccgg gtagaacagc aggccgttgt tcttctcgaa caccggcagc accgacttgc
    12541 gcgacacgct ggtccagcag ccgaacacca cggcgacctt gtccttcacc agcagctgct
    12601 cggccttctc ggcgaacagc ggccagttgg acgccggatc gacgaccacg gcttcgagct
    12661 tcttgccgag cagtccgccc ttcttgttct gctcgtcgat gagcatcagg accgtgtcct
    12721 tgagcgtggt ctcggagatc gcgatggttc ccgacagcga gtgcaacacg ccgaccttga
    12781 tggtgtcggc ggcttgcgcc gcggaggcca tggtcatcgc aacagccgtg ccgagaatcg
    12841 ccaatctgcg
//
LOCUS       DASQJS010000170         4014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2605858, whole genome shotgun sequence.
ACCESSION   DASQJS010000170 DASQJS010000000
VERSION     DASQJS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2605858"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VEW08_07185"
     CDS             <1..618
                     /locus_tag="VEW08_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40555.1"
                     /translation="VAECAKQLSSYGTTLALRYMADFPKAVHAAVLMGTVPASRMPPR
                     YHASAAQAALEQLMVDCAADSACRAMGDMRANLSAALQMVVAIMTAPVFLEKLRNQLY
                     LPASRVHIPNLLVQAAQGDFRGFTGSAGSGRAFADGLYLSITCAESFAHMDVDAAIAV
                     ARATIFGAYRLERQRDACEHWPKGWSSGISTAAPHVTPTPPASQP"
     gene            644..1840
                     /locus_tag="VEW08_07190"
     CDS             644..1840
                     /locus_tag="VEW08_07190"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HYJ40556.1"
                     /translation="MSKHTGLLRSRDTHARVTFVELFFDLVFVFAVTQLSHTLVEHLT
                     LGGALQTLFLLLAVWWVWMYTCWFTNWIDPDKPSVRMLLFTLMLAGLLMSAAIPNAFG
                     HEGLLFALAYAFIQVTRSVFMIFATRRHDPVNHRNFQRITTWLAISAVFWIGGGMAEG
                     YERVAAWVVALSLEIGGPFLGYFVPGLGHSNTTDWKVDGTHMAERCALFVIIALGESI
                     LVTGATAASLPATTAGVCAFLVAFLGSVAMWWIYFNIGAARGSREISGSADPGRYARA
                     VYTYFHIPIVAGIVVSAVADELTIAHPGGHLELPAAMALLGGPALYLAGNVFFKRASA
                     KYYPLSHLVGLGLLAAIAPFALLFTPLALGTATTAVLITVAVWETRSFASGVAPASIA
                     GGGHPE"
     gene            1837..2943
                     /locus_tag="VEW08_07195"
     CDS             1837..2943
                     /locus_tag="VEW08_07195"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HYJ40557.1"
                     /translation="MKYSFVAAGVATAILCAINHARAAAPEGAIDSAAATRFAGLALK
                     CLHQEFPNKIAHTLAGDADVRPPRELFPAFYGCYDWHSAVHGHWLLVRLAQRFPDAAF
                     VPAARAELARSFTKENIAVEAAYLQRAGRASFERPYGLAWLLQLTAELRSWKDPQAQQ
                     WSANLIPLETEAAARIKRWLPDLYYPIRVGEHDQTAFSFGLIWDWAGVAGDAEMRRLL
                     TDAAQRFYAADRNCPLAYEPSGQDFLSPCLAEADFMRRVLDQTAFSRWLKTFLPKIPN
                     DARAEWLTPAVVTNRSDPKLAHLDGLNLSRAWMLDGIASGLALGDRRIAALTDAAKRH
                     RDAALPQVTGEHYEGGHWLGTFAVYLMTRAGSDR"
     gene            3062..3138
                     /locus_tag="VEW08_07200"
     tRNA            3062..3138
                     /locus_tag="VEW08_07200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3096..3098,aa:Arg,seq:acg)
     gene            3316..3861
                     /locus_tag="VEW08_07205"
     CDS             3316..3861
                     /locus_tag="VEW08_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40558.1"
                     /translation="MKRIFAVMALLTTSAFPLVGFAKGNLVRIEVVGANLGAPLVVSD
                     ADTLKQLFIWSGPGAGQPIGSPQSADSGSFIDWHTGIANDVPKGMDTYDVYFFCVFDT
                     QKPDGSLTYSVKYAFDPSTNRGYIYLPGPREKNYGINVSTVFHDVEGHWFNASNRWES
                     LVAPLIRERAVQRVGEVAAAH"
BASE COUNT          676 a         1275 c         1229 g          834 t
ORIGIN      
        1 gtagccgaat gcgctaagca gctgagttcc tacggcacca cgctcgcatt gcgctacatg
       61 gcggactttc cgaaggccgt gcacgccgcg gtgctcatgg gcaccgttcc cgccagccgc
      121 atgccgccgc gttatcacgc atcggcggca caggccgcgc tcgagcagct catggttgat
      181 tgcgccgcgg attccgcgtg ccgcgcgatg ggcgatatgc gcgcgaatct ctccgcggcc
      241 cttcagatgg tcgtcgccat catgaccgct cctgtcttcc tggagaaact gcgcaatcaa
      301 ctctatctac cggccagtcg cgtgcacatt cccaacctgc tggtgcaggc agcacaaggg
      361 gattttcgcg gtttcaccgg gtccgcgggc agcggccgcg cattcgctga cggcctgtac
      421 ctgtcgatca cctgcgccga gtcctttgcg cacatggacg tcgatgctgc gatcgccgtt
      481 gcacgcgcga cgatttttgg cgcgtatcgt ctcgagcgtc aacgggacgc ttgtgagcac
      541 tggcccaagg ggtggtcctc gggtatttcg acagcggctc cgcacgtgac accgacacct
      601 cctgcttcgc agccatgatg ccgccgccat ttggagcttc accttgagta aacacaccgg
      661 cctgctgcgc tcacgcgata cgcacgcgcg cgtcactttc gtagagctct tcttcgacct
      721 ggtgttcgtg ttcgcggtca cgcagctctc gcacacgctg gtcgagcacc tgacgctggg
      781 cggcgcgctg cagacgctgt tcctgttgct ggcggtgtgg tgggtgtgga tgtacacctg
      841 ctggttcacc aactggatcg atcccgacaa accgtcggtg cgcatgctgc tgttcacgct
      901 gatgctggcg ggcctgctga tgtcggcagc cattccgaat gccttcggcc acgaagggct
      961 gctgtttgcg ctggcctacg ccttcatcca ggtcacgcgt tcggtattca tgatctttgc
     1021 cacacgcagg cacgacccgg tcaaccatcg caactttcag cgcatcacca cctggctcgc
     1081 catctcagcc gtgttctgga tcggcggtgg aatggcggaa gggtatgagc gcgtggcggc
     1141 ctgggtcgtc gcgctctcgc tcgaaatcgg cgggccattc ttgggctatt tcgtgccagg
     1201 cctcggccac tcgaacacca cggactggaa agtggacggc acgcacatgg ctgagcgctg
     1261 cgcgctgttc gtcatcattg ccctcggcga atccatcctc gtcaccggcg caacggccgc
     1321 ttcgctgccg gcaaccaccg ccggggtttg cgccttcctc gtggcattcc tcggcagcgt
     1381 ggccatgtgg tggatctact tcaacatcgg cgcggcgcgc ggcagccgtg agatcagcgg
     1441 ctcggcggat ccggggcgat atgcacgagc cgtctacacg tatttccaca ttccgatcgt
     1501 ggcggggatc gtggtgtccg ccgtggccga cgaactcacc atcgcgcacc cgggaggaca
     1561 cctggaactg cccgcggcca tggctttgtt aggcgggccg gcgctgtatc tcgccggcaa
     1621 cgtgtttttc aagcgcgcca gcgcgaagta ctacccgctc tcgcacctgg tcgggcttgg
     1681 cttgttggcg gccatcgcgc cgttcgcgct gttgttcacg ccgctggcac tcggcaccgc
     1741 cacgacagcc gttctcatca ccgtcgcggt ctgggagacg cgctcgttcg cgagcggcgt
     1801 ggctcccgcg agtattgccg ggggagggca tccggagtga agtactcgtt cgttgctgcc
     1861 ggcgttgcga cggcaatcct atgcgcaatc aaccatgcgc gggcggccgc tcctgagggt
     1921 gccatcgatt ccgcggcggc cacccgtttt gccgggctag cgctcaaatg cctgcaccag
     1981 gaatttccta acaagatcgc gcatacgctg gccggtgacg cggacgtccg gccaccgcgt
     2041 gaattgttcc cggctttcta cggttgttat gactggcact ccgccgtgca cgggcattgg
     2101 ctgctcgtgc ggctcgcgca gcgctttccg gacgcagcct ttgtaccggc cgcgcgtgcg
     2161 gagctcgcgc gcagcttcac gaaagagaac atcgccgttg aagccgcgta tctccagcga
     2221 gcggggcgcg cttccttcga gcggccatat ggccttgcgt ggctgctgca gctcacggcc
     2281 gagttgcgct cgtggaaaga cccgcaggca cagcagtggt cggcgaatct catcccgctc
     2341 gagacggaag ccgccgcacg catcaagcgc tggctgccgg atctgtacta cccgatccgc
     2401 gtgggcgaac acgaccagac cgcattctca ttcgggttga tctgggactg ggcgggtgtg
     2461 gccggcgatg cggagatgcg tcggctgctc acagacgccg cgcaacgttt ctacgccgcc
     2521 gaccggaatt gcccgttggc ctacgaacct tcgggacagg attttctgtc accctgtctc
     2581 gcagaagccg acttcatgcg ccgcgtgctc gatcagaccg ccttctcgcg ttggctgaaa
     2641 acgtttctgc ccaagattcc gaacgacgcg cgtgccgagt ggctgacgcc cgcagtcgtc
     2701 acgaatcgca gcgatccgaa gctcgcgcac ctcgatggcc tgaatctcag ccgcgcgtgg
     2761 atgctcgacg gcatcgccag cggccttgcc ctcggcgacc ggcgcatcgc ggcgctgacc
     2821 gatgccgcga aacgtcaccg cgatgcggca ctaccgcagg tgaccggcga acactacgag
     2881 ggtggacact ggctcggcac attcgccgtt tacctgatga ccagagccgg cagcgaccgg
     2941 tagcgtcgct catccggcga tgggggtggg gctgcccaag tacttgttcg gcttggggtt
     3001 atctttgtcc tcgaccccga aaccctttgt ggtaggatcg ccgcccttcg atttgagcca
     3061 cgcgcccgta gctcagctgg atagagtacc tggctacgaa ccaggtggtc gggagttcga
     3121 atctctccgg gcgcgccatc tcccctgatt gaaatcaacg atctgcattc gcagacgttg
     3181 attttttggt cacgtttggt cacgcagagc gaagcgcaga actctaccga ccaactgctc
     3241 gttagagttc caaggcgtgt tcgggcgtat gctcgcggtg ttgagggaag tcaaaagcga
     3301 accaaggaga tggctatgaa acgcatcttc gctgtgatgg ctctgttgac gacgtccgcg
     3361 tttccgctcg tgggtttcgc caagggcaac ctggtcagga ttgaagttgt cggcgccaat
     3421 ctcggtgcgc cgcttgtggt ctccgacgcc gacactctaa aacagctttt catctggtcc
     3481 ggtcccggcg ccggtcagcc aattggctcg ccgcaaagcg ccgattcggg ctctttcatc
     3541 gattggcaca cgggcattgc taatgatgtg ccgaaaggaa tggatacgta cgacgtctac
     3601 ttcttttgcg tcttcgacac acaaaaaccc gacgggagtc tgacgtactc ggtgaagtac
     3661 gcattcgatc catcgacaaa ccgtggctac atctatcttc ccgggccgcg tgaaaagaac
     3721 tacgggatta acgtgagtac agtatttcac gacgttgaag gtcactggtt caatgcatcc
     3781 aaccgttggg aatcgctcgt cgctccactc attcgtgagc gcgctgtgca gcgggtaggt
     3841 gaagtcgctg cggctcattg attcaaattt ttaaggccgg tcacgcgtga ccaacgtgac
     3901 caatgcgtgc ccggattggg cacggcgcgt gaccagcacc tttgcaactc attgaagcgc
     3961 aagggccgcg gagagatcga atctctccgg gcgcgccaat cctttcaagc agtt
//
LOCUS       DASQJS010000171         4038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2921657, whole genome shotgun sequence.
ACCESSION   DASQJS010000171 DASQJS010000000
VERSION     DASQJS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2921657"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2426)
                     /gene="gyrA"
                     /locus_tag="VEW08_07210"
     CDS             complement(<1..2426)
                     /gene="gyrA"
                     /locus_tag="VEW08_07210"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HYJ40559.1"
                     /translation="MNEIAREILPVNLEDEMRQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRILHAMRELGNDWNKAYKKSARVVGDVIGKYHPHGDNAVYDALVRMAQPFSMRYLL
                     VDGQGNFGSVDGDAPAAMRYTEVRMSRIASELLADIEKETVDFQPNYDESETEPTVMP
                     ARFPNLLVNGSAGIAVGMATNVPPHNLTEIINACLAVLDDGEVTLATLMQHVPGPDFP
                     TAGIINGATEIATAYRTGRGRLSIRARTNFEDIDKVGGRQAIIVTELPYQVNKARLLE
                     RIAELVRNKEIDGISELRDESDKDGMRVVIELKRGEVSEVVLNNLFAQTPLETVFGIN
                     MVALIDGRPRLLGLKEMLEAFLRHRREIVTRRTIFDLAKARDRAHILEGLAVALANID
                     EIIALIKASPTPVEAKVKLAAREWSSGVVPQLLARAGDISTRPQGLAAEFGITVVGGG
                     YKLSETQTQAILDMRLHRLTGLEQDKIIGEYQELLALIVDLTDILARPERLMQVIREE
                     LIAIREQYGDARRTEINRDHLDLSTEDLIEPQDVVVTLSHAGYAKSQPVTEYQVQRRG
                     GRGKAATSVKDEDFVEKLFVAHTHDTILCFSNRGKVYWKRVFELPQAGRGSRGKPMVN
                     LLPLEEGERINAVLPIKQFDDDHFVFMATSQGTVKKTPLSAFSRPRASGIIALGLDTE
                     DRLVGVALTDGTREIILSSSGGKSIRFNEEEVRPMGREATGVRGIKLAAGQELISLIV
                     VGEGLVLTASANGYGKLTPLEDFPQHGRGGQGVIALQTTDRNGEMVAALQVNSEHELM
                     MISSTGTLVRT"
     gene            2541..3257
                     /gene="ubiG"
                     /locus_tag="VEW08_07215"
     CDS             2541..3257
                     /gene="ubiG"
                     /locus_tag="VEW08_07215"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003172645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HYJ40560.1"
                     /translation="MSTTQTPNSDAAEIAKFEAAAHRFWDIDGEFKPLHKLNPVRARY
                     VAERCTLAGARVLDVGCGGGLLAETLARAGAQVTAIDLAPSMVETARLHALDSGLTID
                     YRVEEAAALLKSPVKYDVVTCMEMLEHVPDPAATVDVLGKLVRPGGHVFISTINRNFR
                     SFALAIVAAEYVANLVPRGTHEYERLLKPSEVARFARAAGLEVMDIAGLRYDPLREQC
                     SLDSNPSVNYLIHLTRRGTA"
     gene            3254..3946
                     /locus_tag="VEW08_07220"
     CDS             3254..3946
                     /locus_tag="VEW08_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYJ40561.1"
                     /translation="MSGVIKGVLFDLDGTLLDTAQDFHESLNQLRAEEQLAPLSFEIV
                     RAQVSHGGHALVRLGFGTLDPEAHEAMRMRLLNIYRKRLAKYTRLFEGGEEMLGALEQ
                     RGLAWGIVTNKPGWLTEPLLIEVNLHGRVRAVVSGDTLSERKPHPLPLLHAAKTIGVE
                     PGECVFVGDAERDAQAAQAAGMYSVIAGFGYIGDDDRADEWFQHGWLNTPLDLLAWLD
                     EPSRAVKVNGKA"
BASE COUNT          690 a         1267 c         1322 g          759 t
ORIGIN      
        1 gtacgcacca gcgtgccggt agaactaatc atcatgagct catgctcaga attcacttgc
       61 agcgcggcga ccatttcacc gttgcggtcg gtggtctgca gcgcgatgac gccctgcccg
      121 ccgcgaccgt gctgcggaaa gtcctcgagc ggtgtgagtt tgccgtaccc gttggccgag
      181 gccgtgagaa cgaggccttc gccaaccacg atgagcgaga tgagctcctg gcccgcggcg
      241 agcttgatgc cgcgcacgcc ggtggcttcc cggcccatgg gccgcacttc ttcttcgttg
      301 aagcggatgg acttgccgcc gctcgacgag agaatgattt cacgcgtacc gtcagtcagc
      361 gcgactccga ccagcctgtc ttcggtgtcc agccccagcg cgatgatgcc gctggcgcga
      421 ggacgcgaga acgccgacaa cggcgttttc ttgaccgtgc cctggctggt ggccatgaac
      481 acgaagtggt cgtcgtcgaa ctgcttgatg ggcagcacgg cattgatgcg ttcgccctct
      541 tcgagcggca gcaggttgac catgggtttg ccgcgcgagc cacggcccgc ctgcggcagc
      601 tcgaacacgc gcttccagta gaccttgccg cgattgctga aacacagaat ggtgtcgtgc
      661 gtatgcgcga cgaagagctt ctcgacgaag tcttcgtcct tgacggacgt ggccgccttg
      721 ccgcggccgc cgcggcgctg cacctggtat tcggtgaccg gctggctctt ggcgtaaccg
      781 gcgtgcgaca gcgtgaccac cacgtcctgc ggctcgatga gatcttcggt agatagatcg
      841 aggtgatcgc ggttgatctc ggtgcggcgc gcgtcgccgt actgttctcg tatggcaatc
      901 agctcttcgc ggatcacctg catgagtcgt tcgggacggg caagaatatc ggtgagatcg
      961 acgatgagcg ccagcagctc ctggtattcg ccaatgattt tgtcctgctc gagacccgtg
     1021 aggcggtgca ggcgcatgtc gagaatggcc tgtgtttggg tctcggagag cttgtaccca
     1081 ccacccacaa ccgtgatgcc gaattccgcc gcgagaccct gggggcgcgt cgagatgtca
     1141 ccggcgcgcg cgagcagctg cgggaccacg cccgaactcc attcgcgcgc ggccagcttg
     1201 accttcgcct ccaccggcgt gggagaagcc ttgatgagcg cgatgatctc gtcgatgttg
     1261 gcgagcgcga ccgcgaggcc ctcgagaatg tgggcgcgat ccctggcctt ggccaggtcg
     1321 aaaatcgtgc ggcgcgtgac gatttcgcgc cggtgccgca ggaatgcctc gagcatttcc
     1381 ttgaggccga gaagcctggg ccggccgtcg atgagcgcga ccatgttgat gccgaacacg
     1441 gtctcgagcg gcgtctgcgc gaagaggttg ttcaacacca cctcggagac ttcgccgcgc
     1501 ttcagctcga tgaccacgcg catgccgtcc ttgtcggact cgtcgcgcag ttcggagatg
     1561 ccgtcgattt ccttgttgcg taccagctcg gcgatgcgct cgagcaggcg cgccttgttc
     1621 acctggtaag gaagctcggt gacgatgatg gcctggcgcc cgccgacctt gtcgatgtct
     1681 tcgaaattgg tgcgcgcgcg gatcgacagg cggccgcggc cggtgcgata ggcggtggcg
     1741 atttcggtgg cgccgttgat gatgccggcc gtcgggaaat cagggcccgg cacgtgctgc
     1801 atgagcgtgg ccaacgtgac ttcgccgtcg tcgagcaccg ccaggcaggc gttgatgatt
     1861 tccgtgaggt tgtgtggcgg gacgttggtg gccatgccca ccgcgatgcc ggccgaaccg
     1921 ttgacgagaa gattggggaa ccgcgccggc atgaccgtgg gttccgtctc gctttcgtcg
     1981 taattcggct ggaaatcgac ggtttctttt tcgatgtcgg cgagcagctc gctcgcgatg
     2041 cgcgacatgc gcacttcggt gtaacgcatg gcggccggcg cgtcgccgtc caccgagccg
     2101 aagttgccct ggccatcgac cagcaggtaa cgcatggaaa acggctgcgc catgcgcacg
     2161 agggcgtcgt aaaccgcgtt gtcgccgtgc gggtggtact tgccgatgac gtcgcctaca
     2221 acgcgtgccg atttcttgta ggccttgttc cagtcgtttc cgagctcgcg catggcgtgc
     2281 aggatgcgtc gatgcacggg cttcaggccg tcgcgcacgt cgggcagcgc gcggccgacg
     2341 atgacgctca tggcgtagtc gagatacgac tggcgcatct cgtcttcgag attcacggga
     2401 agaatttcgc gcgcgatttc gttcatggaa gagtggtggc agggcccgaa aaaaggtgcg
     2461 gaattctatc acgtaggcct aaccaggaag cctgttttaa cgcgcgaaat accccttgcg
     2521 cgtctatact tccggccctc atgtccacca ctcagacgcc aaacagcgac gccgcggaaa
     2581 tcgcgaaatt cgaagccgcg gcccaccgtt tctgggacat cgacggtgag ttcaagccgc
     2641 tgcacaagct caatcccgtg cgtgcgcgat acgtcgccga acgctgcact ctcgcgggcg
     2701 ccagggttct cgatgtggga tgcggcggcg gattgctcgc cgagacactc gcgcgcgcgg
     2761 gcgcgcaggt tacggccatc gatctcgcac cgagcatggt ggaaaccgct cgcctgcacg
     2821 cgctcgattc cggcctcacc atcgactatc gcgtcgagga ggccgcggcg ctgctgaaga
     2881 gccccgtcaa atacgacgtg gtcacgtgca tggaaatgct cgaacacgtg cccgatccgg
     2941 cagcgacggt ggatgtgctc ggcaagctgg ttcggcccgg tggccatgtc ttcatttcaa
     3001 cgatcaaccg caatttccgg tcgttcgcgc tggccatcgt cgccgccgaa tacgtcgcca
     3061 acctggtgcc gcgcggcacg catgaatacg aacggctgct caaaccatcg gaagtcgcgc
     3121 gcttcgcgcg cgccgccggc ctcgaggtca tggacattgc cggcctgcga tacgatccgc
     3181 tccgcgaaca gtgctcgctc gactcgaatc cctcggtcaa ctacctcatc catctcacgc
     3241 gccgggggac ggcatgagtg gcgtcatcaa aggggtgctg ttcgatctcg atggcacgct
     3301 gctcgacacc gcgcaggatt ttcacgaatc actgaaccag ttgcgcgcgg aagaacaact
     3361 cgcgcccttg tcgttcgaga tcgtgcgcgc gcaggtgtcg cacggcggcc atgcgctggt
     3421 ccggctcggc ttcggcacgc tggatcccga ggcgcatgag gcgatgcgca tgcggctgct
     3481 gaacatctac cggaagcgcc tcgccaagta cacgcgcctc ttcgaagggg gcgaagagat
     3541 gctcggtgcg ctggaacagc gtgggctcgc ctggggcatc gtgaccaaca agccgggctg
     3601 gctcaccgag ccgctgctca tcgaagtgaa tctgcacggt cgggtgcgcg ccgtggtcag
     3661 cggcgacacg ttgtccgaac gcaagccgca cccgcttccc ctgctgcacg ccgcgaagac
     3721 gatcggcgtg gaaccgggcg aatgcgtatt cgtcggggat gccgaacgcg atgcgcaggc
     3781 cgcgcaggcc gccggcatgt attcggtaat cgcgggtttc ggatacatcg gcgacgacga
     3841 ccgcgctgac gagtggtttc agcacggctg gctcaacaca ccgctcgatc tgctcgcctg
     3901 gctcgacgag ccgtcgcgcg cagtcaaggt gaatggaaag gcatgacctc ggcctgggcc
     3961 atcatcctca tcgccgcact cgccgcggcg atcatcggct acctgatcgg cacgctgcgc
     4021 gcggcgaagc gcgccgag
//
LOCUS       DASQJS010000172        19460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1347972, whole genome shotgun sequence.
ACCESSION   DASQJS010000172 DASQJS010000000
VERSION     DASQJS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19460
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347972"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..499)
                     /locus_tag="VEW08_07225"
     CDS             complement(5..499)
                     /locus_tag="VEW08_07225"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ40562.1"
                     /translation="MQFSQKIAPCLWFDSQGEEAANFYVGIFRNSRITSISRYGKAGQ
                     ETHKKPEGSVLTVAFELDGVPFTALNGGPIFKFSEAISLQIFVDDQKELDYYWNKLTP
                     GGDPKAQVCGWLKDKYGLSWQVVPTKMLDWWVKPDERSQRAFEAMMHMGKLDIAALER
                     AYNG"
     gene            complement(613..1974)
                     /locus_tag="VEW08_07230"
     CDS             complement(613..1974)
                     /locus_tag="VEW08_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HYJ40563.1"
                     /translation="MPANRHRAIGTILIVALSGFLMGFDGSLFTGAVGFIQVEFALNE
                     FELGWAVTSMAVAATVSIFLSGPLADRFGRRTVLRLATCIFGVSALLAALSENFATLI
                     VARLLGGLGVGAVLITAPMYIAEISPSAWRGRMVSFNQLFIVLGALAAFVSNYFIVRF
                     GGAADWNWRWMLGIGVVPAIPYFLALLLVPESPRWLAMHNQLPAARGVLARITGSERA
                     DRELEAVLESLEQEAARAKPSLVEILNPALRGVLIVGLLVGILQQITGISSVLSYAVV
                     IFERAGAGANASFMQTVFVGLVNVVFTVLALLLIDRVGRRPLLMFGCFGIGAFMLLAA
                     YGFQVEDEAQSSLLVLIGMLGFVASFAFSLGPGMWVLFSEIFPNRIRGLAISCVGVVN
                     STVCVIVQWVFPWEMKTLGGSHTFLIYGVFAFAGIAFIWRLVPETRGRSLEELEDALV
                     RHT"
     gene            complement(1974..2975)
                     /locus_tag="VEW08_07235"
     CDS             complement(1974..2975)
                     /locus_tag="VEW08_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYJ40564.1"
                     /translation="MANIYEVAELAGVSLATVSRVINPGAKVSEKTRQKVLDAMQQLG
                     FQPNSIAQSLATRTSNAVGVLVSELHGPFFGAMLSAIEQSLKAAGKFVLVAAGHSNEE
                     QEREAIRFLVSRNCDALIVHVERLPDKFLVDHDHNTTPLVVMNRKVRGLADHCFSLNN
                     ELGGYLATQSLVKRKHKRIAYISGPLDWVDAKQRFAGHKRALAEGGIKYDERLLHEGD
                     YHETGGTDALNALFEKNIPFTAVVCANDEMAAGAMAAAHERGLELPADLSIVGFDDAP
                     ISRYVYPKLTTVHYPIADMARMASRWVLKHVYDQPQLEVQQVFEPKLVERDSVGRPA"
     gene            complement(2975..4495)
                     /locus_tag="VEW08_07240"
     CDS             complement(2975..4495)
                     /locus_tag="VEW08_07240"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HYJ40565.1"
                     /translation="MQNLSQTQHRVRRVVIAGGGTAGWMVAAGLSKTLGKLLDIRLVE
                     SDEIGTVGVGEATIPTLVTFHRLLGINEQEFMAATQATFKLGIGFESWRQLPSKEGGE
                     NYIHSFGITGKDHWTAGFQHFWLKGRDRKLATDYGDYCLELRASLESRFAHLPQNGMN
                     YAFHIDAGLYAKFLRKFAEGFGVKRTEGKIVDVKVDETSGNIASIKLDSGDVLEGDLF
                     IDCTGFRGLLIGNALHVGYEDWSHHLFCDSAVALQTESMGPAIPYTRSIAREAGWQWR
                     IPLQHRVGNGMVYCSRYLSDDAAKQGLLANVEGKVLTEPRIIKFRPGQRRQTWRRNCI
                     AIGLASGFIEPLESTSIHLIQRGIMWLLQMFPRDGIRQSDVDEYNRQTNYEIEHIRDF
                     IVLHYHVTRRQDTPFWRSCRAMDIPDSLRHRIQLFRETGRVFRMPSELFAENSWIQVM
                     LGQGIEPLQHHPVADLMGDEELGRFLDGIKSSVEKTVAQLPAHQTYVEQYCRAHSE"
     gene            complement(4507..5520)
                     /locus_tag="VEW08_07245"
     CDS             complement(4507..5520)
                     /locus_tag="VEW08_07245"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HYJ40566.1"
                     /translation="MFPSAERIGEVAGVDPRALPDEVLESTEPLVLRGLATHWPMVRA
                     GLESQAAASAYLRRFNKDATVVVMSGEPEIGGRFFYNDDLSGFNYKMYRTRLDGVLGD
                     LTRLSADTNAASMYVSSTAIDTALPGFRAENDFNFGVRKPVASIWIGNRTRIAAHHDL
                     PDNLACVVAGHRRFTLFPPEQIANLYVGPLEFNPAGQPISLVDFAQPDYDRFPRFAEA
                     QKAARVAELGPGDAIFIPSMWWHHIESLDAFNVLVNYWWRQSPAHMDSPMGALMLALM
                     TVRDLPPAQRSAWENLFRHYVFQANDETVTHIPEYARRALGPLDANLVRELRANVLAR
                     FNR"
     gene            complement(5504..6211)
                     /locus_tag="VEW08_07250"
     CDS             complement(5504..6211)
                     /locus_tag="VEW08_07250"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HYJ40567.1"
                     /translation="MTQHTLLSNTEHRDLRVVTARGAAYGDDVMYSLTFPAEFRSVQA
                     NYPIVFGRSRDGTYTPLALFGFREKQNLFLVDGRWDAPYLPMMIDRQPFLIGASTTGK
                     VVHIDLDHPRVSRTEGERVFDDLGGNTEYLKRAGSMLAAIDEGVSATPAFITAIQEYN
                     LLESFALDIAFSDGTKHRFAGFHTIQEDRLRQLGPDALGKLHSHGYLQAVFMVIASLS
                     NFRELIERASRLDVSQR"
     gene            complement(6219..7235)
                     /locus_tag="VEW08_07255"
     CDS             complement(6219..7235)
                     /locus_tag="VEW08_07255"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HYJ40568.1"
                     /translation="MEVDRRIAEITGIDPHSLPDGLLKSGQPVVLRGLVAHWPMAQAG
                     RAGASEAVAYLRRFEREGAPPAVVTVGPPDIGGRIFYNHDLSDFNFRREHVPLGVVLK
                     TLLKYATQDSPPTIYVASTTIDTWLPGFRAENDLALGVSDPLASIWIGNRTRIPAHQD
                     LPDNLACVVAGRRRVTLFPPQQLSNLYIGPLDFNPGGQAISLVDFDAPDFDRFPRFAE
                     ALKHAQVAELGPGDAVFIPSMWWHHIQALEDFNVLVNYWWRQAPAWMDTPMNTLMLAL
                     MTIRDLPPQQREIWDDVFRHYVFGANESTAAHIPENARRVLGPFDDDKARALRARLLQ
                     RLNR"
     gene            complement(7364..10345)
                     /locus_tag="VEW08_07260"
     CDS             complement(7364..10345)
                     /locus_tag="VEW08_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ40569.1"
                     /translation="MNKTNAFIRRPVAQAISVILGSSLLTPVFAQEANDTPEDVGEIV
                     VTGIRASLESSMNLKRTGQGVVDGIIAEDIGKFPDTNLAESLQRISGVSINRTASGEG
                     QQITVRGVGPDFNLILLNGRQMPASNLGPGGGGTTNSRAYDLSNLASDSVSAIEVYKT
                     SRAETPTGGIGATVNIKTARPLDHPGLVTTFGVKGVYDTSASNLPDSYSGSDLTPEVS
                     AIFSNTFADDKFGIGLSATYQERDSGFSQAAVANGWKTMTGASTDGAHLARPGDPTYP
                     AYTIENPPDATDVYGRPQNFGLSVNGVQRKRTNAQLVLQFAPTDSVTTTLDYTYAENK
                     VQTNRSELSVWFNWGPGTSSWTDGPVVGPIIYSEDMTGSDLSMGGMSLGTKNENNSLG
                     FNVDWEVNDALTLALDYHNSTAESSPDSEFGSAGVLGVAAFIRGTTTIDFSNDFPILN
                     VALPGSVDAADAKVTGSVFQSSFNKSEIQQIQASGRWEFQNNSGLDFGISATEVNNRT
                     AAAVMQQNNWGGLGTEDDYSDDIWTNDNMGGYFDLMSGHNDSRFTDQFLVFDFNRLRA
                     EAAAVAGDEAMFRAPDDYTSDLRSEEKSKSAYVQFTKTFESKMPFHVAAGVRYEETEV
                     FSESLVRIGQTISWDSANEFNIGFAPEQGFDSGTGKYDYVLPSLDLKLDVKDNLVVRA
                     SYSETIGRPGWNDIQGGANLAGQLRFDGGTGSSGDPSLKPLESTNYDLSVEWYYGEGS
                     YASVGYFRKDISNFISIIETVETPFEMHTPAGGALWNEAHTVGGCVVGSANERPCIRD
                     YIFANHPTAPGVTVGAQPGTGSIVGQPGDPIATFRMSRPANMRSDELDGWEFNIQHMF
                     GDSGFGVQANYTMVDSGFKYDDTNTTTPQYPMVGLADSYNVVAFFDKFNWQVRAAYNW
                     RDEFLTATGDGQGANPAYTDAYGQLDLNVTYGVNEHLSVFGEAINLTNETQRLHGRND
                     NQLLFATQIGARYMFGARYKF"
     gene            10534..11418
                     /locus_tag="VEW08_07265"
     CDS             10534..11418
                     /locus_tag="VEW08_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HYJ40570.1"
                     /translation="MIAVTAALFSSACGGAGNDSPPVPSPNPPQPTGPGTPGISETGG
                     IPGGFRLVWADEFDVAGLPDSTKWSYDTERNTAGWYNNELQYYGNARIENSRVENGML
                     AITARREDLSTSMLPDWGGQRYSSARLITRGKASWTYGFIEVRAKLPCGVGTWPAIWM
                     LSAPPQTQWPDDGELDIMEHVGFDPGVVHATAHTGAFNHTRNNHRTATTTIPDACTAF
                     HRYQLHWTANRITVGVDDRNYYQYSNDNSGNAEWPFDSPQFLILNIAIGGDWGGQMGV
                     NDAIFPVTMEVDYARVYQ"
     gene            complement(11450..13096)
                     /locus_tag="VEW08_07270"
     CDS             complement(11450..13096)
                     /locus_tag="VEW08_07270"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40571.1"
                     /translation="MNRRELLLTIGGAALAGSASAQTESAATLPLGPPPSGDALKAWA
                     AKAESLIPELLETDQLPVHLVRAVAAPETPLRFRMETEAPASALAGRQFKRGDSVIVD
                     FEGHRTGYLSFRLATEGREPDAPVRLRFTFGEVPTDVAEPFYPYKGELSEAWLPDELI
                     NVDYLPQAVRMPRRYAFRYVKIEVLDTSPEFRIRFEDLKAHAVTSARGVPAPLTKETP
                     LMQRIDAVSIATLRDCMQTTFEDGPRRDRRLWVGDLRLQALTNYHTFKSNDVVRRCLY
                     LFAALPRADGLVAACVFEKPAARYGGLHIVDYAALFNVTLNDYVEATGDVETGRDLWP
                     AAKRQLEIIGRDVNAEGLYVDPKNMWIFIDWSDKLDRNAAIQGVLVYAYRKTLALARR
                     IGREGEVAEYGARIERMVAAARKHYWDASKRVFVSGPNRQVSWGSQAWMAIAGVSSRR
                     ESADAMRAAVADPNAVRPVTPYLYHYVVEGMLAGGLKKEARALLESYWGGMIAAGADT
                     FWEIYDPAQPLRSPYGDIHINSYCHAWSCTPSYLLRSGLL"
     gene            complement(13281..13820)
                     /locus_tag="VEW08_07275"
     CDS             complement(13281..13820)
                     /locus_tag="VEW08_07275"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HYJ40572.1"
                     /translation="MSKPFTRSTSKVDRGFAPVAMEDAELLILGSLPGQLSLQMQQYY
                     AHPRNVFWKLIERIYERASSLPYMHRVEILTANRIALWDVLAAAERPGSLDSSIVRAS
                     ARANDFAAFFRAHPQISRVYFNGRKAEDLYRRFVLPGVSAEFAAIRYVSMPSTSPAHA
                     GMPFAEKLVRWKRIKEKAP"
     gene            13946..14689
                     /locus_tag="VEW08_07280"
     CDS             13946..14689
                     /locus_tag="VEW08_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HYJ40573.1"
                     /translation="MRQLRTYPVWDAGVRWFHWINVICVLGLVAVGVVILNDRALGVS
                     NDGKVLLKTVHVWIGYVFALNLCWRLVWAFVGGAHSRWRELLPVGRGYPAALRRYVAA
                     LRAGEHPQYLGHNPLGRIAVAVLLFLLLAQAVTGLVLAGTDLFMPPFGASIANGIAAA
                     GVDPRTLVPYAPEMYDKDAYAAMRAMRAPFVRIHGYGFYCLLFAIGAHIAAVVITEVR
                     GGGNLVSAMFTGKKILRAPLSDQTKDASP"
     gene            14807..17650
                     /gene="uvrA"
                     /locus_tag="VEW08_07285"
     CDS             14807..17650
                     /gene="uvrA"
                     /locus_tag="VEW08_07285"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HYJ40574.1"
                     /translation="MTIQQIKIRGARTHNLKNVSVDLPRDKLIVITGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSSYARQFLSMMDKPDVDSIEGLSPAIAIEQKATSHNPRSTVGT
                     VTEIYDYLRLLFARAGIARCPDHGIDLAAQTVSQMVDQIVKMPDGLAVLLLAPIVIDR
                     KGEHAEVFEQLRGQGFVRVRIDGRVHELDALPKIDAKKKHTIEVVVDRLRVRADAAQR
                     LSESLETALKLAGGTARLAPYETDKVTKPEEETLFSARQACPVCGYSVAALEPKMFSF
                     NSPAGACPTCDGIGLKDFFDPLRVVAYPHLSLAGGAIKSWDRKNAYYFALITAMAKHF
                     KFDPETPWEDLSKKAQQVMLFGSGNDEVEFRYVHGNGRATKKKHRFEGIIPNLERRYK
                     ETDSLPVREELAKYLGSQPCPDCNGTRLNRTARNVFVGSHSLPEIAHLTVGDAKKMFS
                     TFDVAGWRAEVADKIVKEVGDRLKFLVDVGLDYLTLDRSAESLSGGEAQRIRLASQVG
                     SGLTGVMYILDEPSIGLHQRDNQRLLATLKHLRDLGNTVMVVEHDEEAIRAADEVLDI
                     GPGAGVHGGKIVAQGTPADIEKHPDSLTGKYLSGRVKIALPGLRTPGDPRRVIKVIGA
                     TGNNLKKVDAEFPLGLFTCVTGVSGSGKSTLVNDTLFTHANHKLNGTVMDMAPCQEIK
                     GLEEIDRIIDIDQSPIGRTPRSNPATYTGLFGPLRELFAEVPESRTRGYDAGRFSFNV
                     KGGRCEACQGDGLIKVEMHFLPDVYVPCDVCQGKRYNRETLDIRWRGKNIHEVLDMNV
                     EDALRLFQNVPGVGSRLQTLMDVGLSYLQLGQSATTLSGGEAQRVKLARELAKRATGR
                     TLYILDEPTTGLHFHDVAQLLDVLHRLRDEGNTVVVIEHNLDVIKTADWIIDLGPEGG
                     AGGGTILVTGTPEEVARNKDSYTARYLQPLLVP"
     gene            17892..>19460
                     /locus_tag="VEW08_07290"
     CDS             17892..>19460
                     /locus_tag="VEW08_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYJ40575.1"
                     /translation="MNDFNSFRRGLSWCATALLATLAAGCTGDSILGAGGRVATAPIA
                     PVVTAVSPLDNAIGVAVGSTVAITFDKAMVAGTTFTVTCAAPCVNPNGTVALSAGGTV
                     ATYTLAATTSFAPLTLYTVTANAPMSAGGLPLVTPFVSHFITAGTTADTNRPRVLNTS
                     PLTSSPGPTLAVATNASVAAVFSKDMNQASIEATAPATFTVTCVTPCVSPTGVVSYSV
                     GSRTAVFIPSAPLTTATTYTATISTAAMDLAGNALAGNQAALPAASAYSWTFTTAAAA
                     ATGAITVLSTSPLAAATGVCPSATVNATFTVPSGLRLNPLTVNATNFTVVGPAPGLVP
                     VTAATISVDSATGRIATFTPAAALAANTSYSATLIGGATGVKDLAIPANAMAANSTWT
                     FTTGAATCVTPPAIPLNSAAVFGTFGGTAGMTNSGILTVVNGNIGTIATGTSSITGFH
                     DTAGDIYTESIGANAGAVNGTIYTCTNSTTGPTSAGPNAVKCAVATQARLDAQAAYLQ
                     LAGLAPGANPGANLG"
BASE COUNT         3738 a         6120 c         5984 g         3618 t
ORIGIN      
        1 gctgttagcc attgtaggca cgttcgagcg cggcgatgtc gagcttgccc atgtgcatca
       61 tggcctcgaa ggcacgctgc gatctctcat cgggcttgac ccaccagtcg agcattttcg
      121 tcgggaccac ctgccaggac aggccgtact tgtccttgag ccagccgcaa acctgcgcct
      181 tggggtcgcc gccgggggtc agtttgttcc agtagtagtc gagctccttc tggtcatcga
      241 cgaagatctg caacgagatg gcctcggaaa acttgaagat cggcccacca ttgagcgcgg
      301 taaatggcac accgtcgagc tcgaaagcca cggtcagcac cgagccctcg ggcttcttgt
      361 gcgtttcctg acccgccttg ccgtagcgcg agatggaggt gatgcgcgaa ttcctgaaaa
      421 tgccaacgta aaaattggca gcttcttcgc cctggctgtc gaaccacagg cagggagcaa
      481 tcttctggct gaattgcatg aatgtgtctc cttcgtgatg tgttcactgc atagccgaat
      541 tggggcaacg cagatcgaca gcctccggaa attattgaat cgaggcggac ccgactgtcg
      601 gacagcgccg gctcaggtgt gccgcaccag agcgtcttct agttcttcga gactacggcc
      661 gcgggtttca ggcaccaggc gccagatgaa ggcgattccc gcgaacgcga acaccccgta
      721 gatcaggaag gtgtgcgagc caccgagcgt tttcatctcc cacggaaata cccactgcac
      781 gatgacacac accgtggaat tcacgacgcc cacgcaggaa atcgcgagcc cgcggatccg
      841 gttgggaaag atctccgaga acaacaccca cattcccggt cccagcgaga acgcaaaact
      901 cgcgacgaac cccagcatgc cgatcagcac cagcagtgag ctttgtgctt cgtcttcgac
      961 ctggaaaccg taggccgcca gcagcatgaa tgcgccaatt ccaaagcaac cgaacatcaa
     1021 caagggcctg cggccaaccc ggtcgatgag caacagcgcc agcactgtga acaccacgtt
     1081 cacgaggccc acgaacaccg tctgcatgaa tgaagcattc gcgccggctc cggcgcgctc
     1141 gaatatcacg acggcgtacg aaagcacgga gctgatgccg gtgatctgct gcaggatgcc
     1201 aaccaacaag ccgacgatca gcacgccacg cagagcgggg ttgaggattt ccacgagcga
     1261 gggtttagca cgcgccgctt cctgctcgag tgactcgagc accgcttcca gctcgcggtc
     1321 agcgcgctcg gagccagtga tgcgcgccag cacgccacgc gcggcgggca actggttgtg
     1381 catcgccagc cagcgcggac tctctggcac caacagtaat gccagaaaat atgggatcgc
     1441 cggcaccacg ccgataccga gcatccagcg ccagttccag tccgcggcgc cgccgaaacg
     1501 cacgatgaag tagttgctga cgaaggcagc cagcgctccg agcacgatga acaactggtt
     1561 gaaggacacc atgcggccgc gccatgcaga cggcgaaatt tccgcgatgt acatcggcgc
     1621 ggtgatgagc acggcgccga ctcccaaacc acccaacagc cgcgcaacga tcagcgtcgc
     1681 gaagttctcc gacaacgccg cgagcaaagc cgacacgccg aatatgcagg tcgccagacg
     1741 caggaccgtg cgccgcccga agcgatcggc gagtggaccc gagaggaaaa tggatacggt
     1801 ggcggccacg gccatcgagg tgacggccca accgagctcg aactcgttca gcgcgaactc
     1861 gacctggatg aacccgacgg cgccggtgaa cagcgatccg tcgaagccca tgagaaaacc
     1921 gctgagtgcc acgatcagga tggtcccgat cgcgcggtgg cggttcgccg gcatcacgca
     1981 ggccggccga cggaatctcg ctccacgagc ttgggttcga acacctgctg tacttcgagc
     2041 tgcggctggt cgtacacgtg cttgagaacc cagcgcgacg ccatccgggc catgtcggcg
     2101 atcggatagt gaaccgtggt gagtttcggg tagacgtagc gggaaatggg cgcgtcgtcg
     2161 aacccgacga tggacaggtc ggcgggcaac tcgagcccgc gctcgtgcgc ggccgccatc
     2221 gcaccggcgg ccatctcgtc attggcgcag accacggcgg tgaacggaat attcttctcg
     2281 aacagcgcat tcagagcgtc ggtaccgccg gtctcgtggt agtcgccctc gtgcagcagg
     2341 cgttcgtcgt atttgatgcc gccttccgca agcgcgcgct tgtggccggc gaagcgctgc
     2401 ttggcatcca cccagtcgag cggaccggag atgtaggcga tgcgtttgtg tttacgcttc
     2461 accagcgact gcgtggcgag gtagccaccg agctcgttgt tgagactgaa gcagtgatcg
     2521 gcgagcccgc gcaccttgcg gttcatgacc accagcggcg tcgtgttgtg atcgtgatcg
     2581 acgaggaact tgtccgggag ccgctcgacg tgaacgatga gcgcgtcgca gttgcggctc
     2641 acgaggaagc ggatcgcttc gcgttcctgc tcttcgttgc tgtgtccggc ggccaccagc
     2701 acgaattttc cggccgcctt cagcgactgc tcgatggcgc tcagcatggc gccgaagaac
     2761 ggaccatgca gctccgagac cagcacgccg accgcattcg aggtgcgcgt ggccagcgac
     2821 tgcgcaatgg aattgggctg gaagcccagc tgttgcatgg catcgagcac cttctggcga
     2881 gtcttctcgc tgaccttggc acccggattg atgactcgcg aaacggtggc gagggaaacg
     2941 cccgccagct ccgcgacttc atagatgttg gccattactc gctatgcgcc ctgcaatatt
     3001 gctcgacgta ggtctggtgc gcgggtagtt gggccacggt cttctcgacc gaagacttga
     3061 tgccgtccag aaaacgcccc aactcctcgt ctcccatcag atcggccact ggatgatgct
     3121 gcagcggctc gattccctgc cccaacataa cctgaatcca cgagttttcg gcgaatagtt
     3181 cgctcggcat gcggaaaacc cggcccgttt cccggaacag ctggatgcga tgtcgcaggg
     3241 agtcaggaat gtccatggca cgacatgatc gccagaatgg cgtgtcctgc cggcgagtca
     3301 cgtggtagtg cagcacgatg aaatcgcgaa tatgttcgat ctcgtagttg gtctgccggt
     3361 tgtactcgtc gacgtcggac tggcggatgc cgtcccgcgg gaacatctgc aggagccaca
     3421 tgatgccgcg ctggatcagg tgaatgctgg tcgattccag cggctcgatg aacccgctgg
     3481 cgaggccgat ggcaatgcag ttccgtcgcc aggtctggcg gcgctggccg ggccggaact
     3541 tgatgatccg cggctcggtg agcaccttgc cttccacgtt cgccaacaaa ccttgtttgg
     3601 ccgcatcgtc ggaaaggtag cggctgcagt acaccatgcc gttgccaacg cgatgctgca
     3661 gcgggatgcg ccactgccag ccggcctcgc gggcgatgga ccgcgtgtaa ggtatggccg
     3721 gccccatgga ctcggtctgc agcgccactg cgctgtcgca gaacaggtgg tgagaccagt
     3781 cctcgtagcc cacgtgcagc gcgttgccga tgagcaggcc gcgaaagccc gtgcagtcga
     3841 tgaacaggtc gccttcgagc acatcgccgg aatcgagctt gatggaggcg atgttgcccg
     3901 acgtttcatc caccttcaca tcgacgatct tgccttcggt gcgcttgacg ccgaatcctt
     3961 cggcaaattt gcgcaggaat ttcgcgtaca ggccggcatc gatgtggaaa gcgtagttca
     4021 tgccgttctg cggcaaatgc gcgaatcggc tttcgagcga cgcacgcagc tccaggcagt
     4081 agtcgccgta gtcggtggcc agcttgcggt cgcggccctt gagccagaag tgctggaagc
     4141 ccgccgtcca gtggtctttg ccggtgatgc cgaaggaatg aatgtagttt tcgccgccct
     4201 ctttcgaagg aagctggcgc cagctttcga agccgatgcc gagcttgaat gtagcctgcg
     4261 tggcggccat gaactcctgc tcattgatcc ccagcaggcg atgaaacgtc acgagagttg
     4321 gaatggtcgc ttcgcccacg ccgactgtcc cgatctcatc ggactccacc agcctgatgt
     4381 cgagcagctt gccgagcgtc ttcgacagcc ccgcagccac catccagccc gccgtaccac
     4441 cgccggcgat gactacgcgt cgcacccggt gctgcgtctg gctcaggttc tgcatgccca
     4501 taccttctat ctattgaatc gtgcgagtac gtttgcgcgc aattcgcgca cgaggttcgc
     4561 gtccaggggc ccgagcgcgc gccgcgcgta ttcgggaatg tgggtcaccg tttcgtcgtt
     4621 cgcctggaat acgtagtgcc ggaacaggtt ttcccacgct gagcgctgcg ccggcggaag
     4681 gtcgcgcacc gtcatcaatg cgagcatcag cgcgcccatc ggtgagtcca tgtgcgccgg
     4741 agactggcgc caccagtagt tgaccagcac gttgaaagca tcaagtgact cgatgtggtg
     4801 ccaccacatg ctcgggatga agatggcatc gccggggcca agctcggcaa cacgggcggc
     4861 cttctgcgcc tcggcaaaac gcggaaagcg atcgtagtcc ggctgggcga agtccacgag
     4921 actgatcggc tgtccggcgg ggttgaactc gagcggcccc acgtacaggt tggcaatctg
     4981 ttccggcgga aacagcgtga agcggcgatg ccccgcgacg acgcaggcaa ggttgtcagg
     5041 caggtcatga tgcgcggcaa ttcgtgtgcg gttgcctatc cagatgctcg ccaccggctt
     5101 gcgtacgccg aaattgaagt cgttctccgc gcgaaaaccc ggcagcgccg tgtcgatggc
     5161 ggtggagctc acgtacatcg atgccgcgtt cgtgtcggcc gaaaggcgcg tgaggtcgcc
     5221 gagcacgcca tcgagccgcg tgcggtacat cttgtagttg aagccggaaa ggtcgtcgtt
     5281 gtagaaaaag cgcccgccaa tttcgggctc accggacatg acgaccacgg tcgcatcctt
     5341 gttgaaacgc cgcaggtagg ccgacgcggc cgcctgcgat tccagtcccg cacgcaccat
     5401 gggccagtgg gtggcgagtc ctcgcagcac cagcggctcg gtggactcca gcacctcatc
     5461 gggcaacgcg cgtgggtcca cgcccgccac ctcgccgatt cgctcagcgc tgggaaacat
     5521 cgagccggct cgcgcgctca atcagctcac gaaaattcga aagcgaggcg atcaccatga
     5581 acaccgcctg cagatagccg tggctgtgca gtttgccgag cgcatcgggg ccgagctgtc
     5641 tcaggcgatc ttcctggatc gtgtggaagc ccgcgaaccg gtgctttgtg ccatcgctga
     5701 acgcgatgtc gagcgcgaac gactccagca ggttgtattc ctggatcgcg gtgatgaatg
     5761 ccggcgtcgc gctgacgcct tcgtcgatcg ccgccagcat ggaacccgcg cgcttgagat
     5821 attcggtatt gccgccgagg tcgtcgaaca cccgctcgcc ctcggtgcgg ctgacgcgcg
     5881 gatgatcgag atcgatgtgt acgaccttgc cggtggtcga tgcaccgatg agaaacggct
     5941 gtcgatcgat catcatcggt agatagggcg cgtcccagcg gccgtcgacg aggaacaggt
     6001 tctgcttctc gcggaagccg aacagggcca gcggcgtgta ggtgccatcg cgcgaccggc
     6061 cgaacacgat gggatagttc gcctgcacgc tgcggaattc ggccggaaac gtgagcgagt
     6121 acatcacgtc gtcaccgtag gcagcgcctc gggcggtgac cacgcgcaag tcgcgatgtt
     6181 cggtgttgct cagaagcgta tgttgagtca cgagttcctc agcggtttag gcgttgaagc
     6241 aggcgcgccc gaagtgcccg tgccttgtcg tcgtcgaacg ggccgagcac gcggcgcgca
     6301 ttctccggta tgtgcgcggc agtggattcg ttggcgccga acacatagtg gcgaaaaacg
     6361 tcgtcccata tttcgcgctg ttggggcggc agatcgcgaa tcgtcatgag agcgagcatc
     6421 agcgtgttca tcggcgtatc catccaggcc ggcgcctgcc gccaccagta gttgaccagc
     6481 acgttgaagt cctcgagcgc ctggatgtga tgccaccaca tgctcggaat gaacaccgcg
     6541 tcgccgggac cgagctccgc gacctgcgcg tgtttgagcg cttcggcgaa cctggggaag
     6601 cgatcgaagt ccggtgcgtc gaagtcgacc aggctgatgg cctggccgcc tggattgaaa
     6661 tcgagtggcc cgatgtacag gttggacaat tgttgtggag gaaacagagt aacgcgccgc
     6721 cgtccggcga ccacgcaggc aaggttgtcc ggcagatcct gatgcgcggg aatgcgcgtg
     6781 cgattgccga tccagatgct ggcgagcgga tcgctgacgc caagcgcgag gtcgttctcg
     6841 gcgcggaaac ctggcagcca ggtgtcgatg gtggtggaag ccacgtagat cgtgggcggc
     6901 gagtcctggg tggcgtattt gagcaacgtc ttcagcacca cgcccagtgg cacatgttcc
     6961 cgccggaaat tgaaatcgct caggtcgtgg ttgtaaaaaa tgcgcccgcc gatatccggc
     7021 ggtccgacgg tgaccaccgc cggcggcgcc ccttcccgtt cgaagcgccg gaggtacgcc
     7081 acagcttcgc tggcccccgc acgcccggcc tgcgccatgg gccaatgcgc gacgagtcca
     7141 cgcagcacca ccggctgccc ggacttcagc aacccgtcgg gcagcgaatg cggatcgatg
     7201 cccgtgattt cggcgatgcg cctgtcgact tccatattgt gatgagaatc taccggcaat
     7261 tcgtcgatgg gaacaggaag cccaaaaaaa ggagccgctg acgggcagcg gctcctccct
     7321 ggcatacgtc actatgaccg cataccagtc ttcttctccg cactcagaac ttgtatcgag
     7381 ctccgaacat gtatcgggcc ccgatctggg tcgcgaacaa cagctggttg tcgttacgtc
     7441 catgtagccg ctgcgtctca ttggtgaggt tgatggcctc accgaacacg gacaggtgtt
     7501 cgttgacccc ataagtcaca ttgagatcca gctgaccgta agcatcggtg taggcagggt
     7561 tcgcgccctg accatcgccg gtagccgtca ggaactcatc gcgccagttg taggcggcac
     7621 gaacctgcca attgaacttg tcgaagaacg ccaccacgtt gtacgaatcc gccagtccca
     7681 ccatcggata ctgaggagtc gtcgtattgg tgtcgtcgta cttgaagccc gaatcgacca
     7741 tcgtgtagtt agcctggacg ccaaagccgc tgtcgccgaa catgtgctgg atgttgaatt
     7801 cccatccgtc cagttcgtcc gaccgcatgt tcgccggtct cgacatccgg aatgtcgcaa
     7861 tcggatcacc tggctggcca acaatgcttc ccgttccggg ttgagctcct accgtaacgc
     7921 ccggagccgt tgggtgattc gcgaaaatgt agtctcggat gcacggcctc tcgttggcag
     7981 agccaacgac acaaccgcca acggtgtgag cctcgttcca taacgcgcct ccggcgggcg
     8041 tatgcatttc gaacggagtc tcaacagttt cgatgatgct gatgaagttc gagatgtcct
     8101 tgcggaaata gccaaccgac gcgtagctgc cttcgccgta ataccactcg acagaaaggt
     8161 catagttggt cgattccagc ggcttgagac tgggatcacc cgacgaaccg gtaccaccgt
     8221 cgaagcgaag ctgaccggcg aggttggctc cgccctggat atcgttccag ccaggtcgac
     8281 cgatggtctc gctgtagctg gcgcgcacca ccaggttgtc cttcacgtcg agtttcagat
     8341 ccagagacgg cagcacgtag tcgtatttgc cagtgcccga gtcgaaccct tgctcgggtg
     8401 caaacccgat gttgaattca ttggccgagt cccacgaaat agtctgtccg attcgcacca
     8461 gtgactccga aaagacttcg gtttcctcgt aacgaactcc cgccgcgacg tggaacggca
     8521 tcttgctctc gaacgtcttg gtgaactgca cgtacgcgct cttggacttc tcttcgctgc
     8581 gcaggtcgct cgtgtagtca tccggggcac ggaacatggc ctcgtcaccg gcgaccgcag
     8641 ccgcctctgc gcgcaggcgg ttgaaatcga acaccaggaa ctgatccgtg aatcgggaat
     8701 cgttgtgtcc cgacatcaga tcgaaataac cacccatgtt gtcgttcgtc cagatgtcat
     8761 cgctgtagtc gtcctcggtg cctaggccgc cccagttgtt ctgctgcatg acggccgctg
     8821 cagtgcggtt gttcacctcg gtcgcgctga ttccgaaatc cagcccggag ttgttctgga
     8881 actcccaacg tccgcttgcc tggatctgct ggatctcgga cttgttgaag ctgctctgga
     8941 acaccgatcc cgtgaccttc gcgtcagccg cgtcgaccga gccgggcagt gcgacgttca
     9001 ggatcggaaa gtcattggaa aaatcgatcg tggtggtgcc gcggatgaag gctgcaacgc
     9061 ccagcacgcc ggcggaaccg aactcgctgt ctggcgacga ttcggcggtg gagttgtgat
     9121 agtcaagcgc cagggtcagc gcatcgttca cttcccagtc gacgttgaag ccaagcgagt
     9181 tgttttcgtt cttcgttccc aggctcatgc cgcccatgga caggtctgag ccggtcatgt
     9241 cttccgaata gatgatcggg cccacgacag gcccatcggt ccacgaactg gttcccggcc
     9301 cccagttgaa ccacaccgac agctcgctgc ggttcgtctg gaccttgttc tcggcatagg
     9361 tgtagtcgag cgtcgtggtg acgctgtcgg tcggcgcgaa ctgcagcacc aattgggcgt
     9421 tggtgcgctt ccgctgcacg ccgttgacgc tcaatccgaa attctgagga cgtccgtaga
     9481 catcggtggc gtccggcgga ttttcgatcg tataggctgg gtaggtaggg tcacccggtc
     9541 gcgccagatg tgccccgtcg gtcgaggcgc cggtcatcgt cttccagccg tttgccacgg
     9601 cggcctggct gaatcctgaa tcgcgctcct gataagtcgc gctcaggccg atgccgaact
     9661 tgtcgtcggc gaaggtgttg ctgaagatgg ccgacacttc aggcgtaaga tcgctgcccg
     9721 agtagctgtc gggcaggttg gacgcggaag tatcgtagac gcctttcacg ccaaaagtgg
     9781 tgacgaggcc ggggtggtca agcggacgcg ccgtcttgat gttcaccgtt gcgccgatgc
     9841 cgcctgtggg cgtctcggca cgggaggtct tgtacacctc gatcgcagac accgaatcag
     9901 acgccaggtt ggaaagatcg taggcgcgcg agttcgtcgt gccgccgcca cccgggccga
     9961 ggttggaagc cggcatctgc cggccgttga gcaatatcag attgaagtcg gggccaacgc
    10021 cgcgaaccgt gatctgctgc ccctcaccac tcgccgtgcg attgatggat acgccgctga
    10081 tgcgttgcag cgactccgcg agattggtat ccgggaattt gccgatatct tcggcgatga
    10141 ttccgtcgac gacgccctgg cctgtgcgct tgagattcat cgatgattcg agtgatgcgc
    10201 gaataccggt gacgacgatc tcgccgacat cctccggagt atcgttcgcc tcttgcgcaa
    10261 agaccggcgt caagagagat gatccgagga tcaccgaaat cgcctgcgcg acaggcctgc
    10321 gaatgaatgc gttcgtcttg ttcatatgcc ccctcagtaa aaaaggtgta agcgtcagac
    10381 aaaaaaatgt aagcgcttac aatttttacg ctagcatgca ctccgaactc aggcaagcaa
    10441 tcaagtgctg acaaacccat gtcttgtatg tcttttttga tgtcttcaat ttcgactttc
    10501 attagtcaaa cgaattcagc ccgctggttt gccttgatag cggtaaccgc ggcacttttc
    10561 tcgtcggctt gcggaggtgc aggcaacgac agtccccctg ttccctcacc taatcctccg
    10621 cagccgacgg gacctggcac gccgggtatt tccgaaactg gcggaattcc cggcggattt
    10681 cgtctcgtct gggccgatga gttcgatgtc gcgggcctgc ccgactccac gaagtggagc
    10741 tatgacaccg agcgcaatac ggcaggctgg tacaacaacg agctgcagta ctacggcaac
    10801 gcgcgtatcg aaaattcgcg cgtcgaaaac gggatgctgg ccatcaccgc gcgccgcgaa
    10861 gacctatcta cctcgatgtt gccggactgg ggcggacagc gttattcctc ggcacggctc
    10921 atcacgcgcg gcaaggcaag ctggacctac ggcttcatcg aggtgcgcgc caaactaccc
    10981 tgcggcgtcg gcacctggcc cgcgatctgg atgttgtccg ccccaccaca aacccagtgg
    11041 cccgacgacg gcgagctcga catcatggag cacgtgggct tcgaccccgg cgtggtgcac
    11101 gccaccgcgc acaccggcgc gttcaaccac acgcgcaaca accatcgcac cgccaccacc
    11161 acgatccctg acgcctgtac tgcgttccac cggtaccagc tgcactggac ggcgaatcgc
    11221 attaccgtgg gtgtcgacga tcgcaactac tatcaatact cgaacgacaa ctccggtaat
    11281 gccgaatggc cttttgacag cccgcagttc ctgatcctca acatcgccat cggcggcgac
    11341 tggggcgggc agatgggcgt caacgacgcc attttcccgg tgaccatgga agtggactac
    11401 gcgcgcgtct accagtaggt gccgggtgag gaaagtgggg agtggctcat cacagcagcc
    11461 cgctgcgcag tagataggac ggcgtgcagc tccaggcgtg gcagtagctg ttgatgtgaa
    11521 tgtcgccgta cggtgaacgc agcggctgcg ccggatcgta tatttcccag aaggtgtcgg
    11581 cgcctgcggc gatcatgccg ccccagtacg actccagcag ggcgcgcgct tctttcttga
    11641 ggccgcccgc cagcatcccc tcgaccacgt agtggtacag ataaggagtg accggccgga
    11701 ccgcatttgg atccgcgacc gcggcgcgca tggcatcggc actttcgcga cgcgaggaaa
    11761 cgccagcgat ggccatccac gcctgcgagc cccacgaaac ctggcggttc ggaccgctca
    11821 cgaacacgcg cttggacgca tcccagtagt gtttgcgcgc cgcggcgacc atgcgctcga
    11881 tgcgtgcgcc gtattcggcg acttcgcctt cccggccgat tcggcgagcc agcgccagcg
    11941 tcttccggta cgcgtagacc agcacaccct gaatggccgc gttccggtcg agcttgtcgg
    12001 accagtcgat gaagatccac atgttcttcg gatcgacgta gagaccttcg gcattcacgt
    12061 cacgaccgat gatctcgagc tggcgcttcg cggccggcca caggtcgcgg ccggtttcga
    12121 catcaccggt ggcttccacg tagtcgttga gcgtcacgtt gaacagcgcg gcgtagtcga
    12181 cgatgtgcag gccgccgtat cgggccgctg gcttttcgaa gacgcacgcc gccaccagcc
    12241 cgtcggcgcg cggcaaggca gcgaagagat acaggcagcg ccgcacgacg tcgttggact
    12301 tgaatgtatg gtagttagtg agcgcctgca ggcgaaggtc accgacccac agccgccggt
    12361 cgcggcgcgg gccgtcttcg aacgtggtct gcatgcagtc gcgcagcgtg gcgatcgaca
    12421 ccgcgtcgat gcgctgcatg agcggcgttt ccttcgtcag cggcgccgga acgccgcgtg
    12481 cggacgtgac cgcgtgggcc ttcaggtcct cgaaacgaat ccggaattcc ggcgacgtat
    12541 ccagaacctc aatcttcacg tagcggaacg cgtaccgccg cggcatccgc acggcctgag
    12601 gaaggtaatc gacattgatc agctcgtcgg gcagccaggc ctcgctcaac tcgcctttgt
    12661 aaggatagaa aggctcggcg acatccgtcg gaacttcacc gaacgtgaag cgcaggcgaa
    12721 ccggggcgtc cggctcgcgc ccctccgtgg ccaggcgaaa tgagagatag cccgtgcgat
    12781 ggccttcgaa gtcaacgatg acgctgtcgc cacgcttgaa ttgccggccc gcgagcgccg
    12841 atgccggcgc ttcggtttcc atgcggaacc gaagcggcgt ttcgggagct gcaacggcac
    12901 gcaccaggtg caccggcagt tggtcggttt cgagcagctc aggtatcagc gactcggcct
    12961 tggccgccca ggctttcagc gcgtcgcccg agggtggtgg cccgagcggc agcgtcgcag
    13021 ccgattcagt ctgcgcggac gccgaacccg cgagggcggc gcctcctata gtcaacagca
    13081 gttctcggcg attcatggcg ccacattaac gtggcacctc cgagccgtca ccgcgtttta
    13141 catttatccg cgccagcgca gtgtccgccc ggctcgatgc gtcctttgac gagtcgccag
    13201 cccgccttcg ggtgctcgaa agcgagaatc cgctgcagcg aaggatcgcg aaataccact
    13261 cgcaggcctt gtccgcaagc tcatggggct ttctccttga tccttttcca tctcactaac
    13321 ttctctgcaa agggcatgcc cgcgtgcgcg gggctggtcg atggcatgga cacataacgg
    13381 atcgcggcga attcggctga caccccgggc agcacgaatc gccggtacag gtcctcggcc
    13441 ttgcggccat tgaagtagac gcggctgatc tgcggatggg cccggaagaa tgccgcgaaa
    13501 tcgttcgccc gcgccgacgc gcggacgatc gaactatcga ggctgccggg tcgctcggcc
    13561 gcggccagaa catcccatag ggcaattcga ttggccgtca atatttctac tcggtgcatg
    13621 tagggcagcg atgacgctct ttcgtagatc cgctcgatga gtttccagaa cacattccgg
    13681 ggatgcgcgt agtactgctg catctgcagg gaaagctgcc cgggaagcga gccgaggatg
    13741 agcaattcgg cgtcttccat cgcgacgggg gcgaatccgc ggtcaacctt cgacgtcgat
    13801 cgagtgaatg gcttgctcac gtgcttgccg gattgagtct ttcaggagtc ggctgggtca
    13861 cgaccgtgtg cctcaatacg tgtgaatacg aacgcgaacc cgcgccggtt cgctcatagt
    13921 gaactcccga cggagagttc gatcgatgcg ccaattgagg acctatcctg tttgggacgc
    13981 cggcgtacgc tggttccact ggatcaacgt catctgtgtg ctcggcctcg tcgccgttgg
    14041 cgtggtgatt ctcaacgaca gagcgctggg tgtcagcaat gacggaaaag tcctgctgaa
    14101 gacggtgcac gtctggatcg gctacgtgtt cgcgctcaat ctttgctggc gcctggtctg
    14161 ggcattcgtc ggcggcgccc attcacggtg gcgcgagctc ctgcctgtcg gtcgcggcta
    14221 tcccgcagcg ttgcgcaggt atgtcgctgc gttgcgcgcc ggtgaacacc cacaatatct
    14281 cggacacaac ccattgggtc gcatcgcggt ggcggtgttg ttgttcctgc tgctggcgca
    14341 ggcggtcacc ggcctggtgc ttgccggcac cgaccttttc atgccgccct tcggtgcctc
    14401 gatcgcaaac ggcatcgcag ccgccggagt ggatccgcgc acgctggtac cgtatgcgcc
    14461 ggagatgtac gacaaggacg cctatgcagc catgcgcgcc atgcgtgcac cgttcgtacg
    14521 cattcatggt tatggcttct attgtctgct gttcgcgatc ggcgcccaca tcgccgcggt
    14581 cgtgatcacc gaagtgcgcg gtggcggtaa cctggtgtcg gcgatgttca cgggaaagaa
    14641 gattctgcgg gcgccgctat cggaccagac caaggacgca agcccctgat ccaacgtagg
    14701 taacggcgtt catacagtac tcagcgcggc caagtactgg ttatgcacgg gccggggtcg
    14761 ccgattaaac tccgcggttt ctctccactc gcacgagaaa ccgcgcttga ccatccagca
    14821 gataaagatt cgtggcgccc gcacgcacaa tctcaagaac gtctccgtcg acctgccgcg
    14881 cgacaaactc atcgtgatca ccggtttgtc cggctccgga aaatcctcgc tggccttcga
    14941 cacgttgtac gcggaaggcc agcgccgcta cgtcgaatcc ctgtcgtcat atgcgcggca
    15001 gttcctttcg atgatggaca agccggacgt cgacagcatc gagggtctgt cgcccgccat
    15061 cgccatcgaa cagaaggcga cctctcacaa tccgcgctcc accgtcggca cggtcacgga
    15121 gatctacgac tatctacgcc tgctgttcgc acgcgcgggc atcgcgcgct gccccgatca
    15181 tggcatcgat ctcgcggcgc agaccgtgag ccagatggtg gaccagatcg tgaaaatgcc
    15241 ggatggcctg gcggtgctgc ttctggcgcc catcgtcatc gatcgcaagg gcgaacacgc
    15301 ggaagtattc gagcaactcc gtggccaggg tttcgtgcga gtgcgaatcg acggccgcgt
    15361 gcatgagctc gatgcgctgc cgaagatcga cgccaagaag aaacacacca tcgaggtagt
    15421 ggtggacagg ttgcgcgtcc gcgccgacgc cgcacagcgc ctgtcggaat cgctggagac
    15481 ggcgctgaag ctcgccgggg gcaccgcgcg actcgcgccc tacgaaacag acaaggtcac
    15541 gaagcccgaa gaggaaaccc tgttctccgc gcgccaggct tgccccgttt gcggctacag
    15601 cgtggcggcg ctcgagccga aaatgttttc gttcaacagc cccgcgggcg cctgccccac
    15661 ctgtgatggc atcggcctca aggatttctt cgatcccctt cgagtcgtcg cctatccaca
    15721 cctgtctctc gcggggggcg ccatcaagag ctgggatcgc aagaacgcct attacttcgc
    15781 gttgatcacc gcgatggcga aacacttcaa gttcgatccg gaaacaccgt gggaagacct
    15841 gtcgaagaaa gcacagcagg tcatgttgtt cggcagcggc aacgacgaag tggagtttcg
    15901 ctacgttcac ggcaatggtc gcgccacgaa aaagaaacat cgcttcgagg gcatcattcc
    15961 gaacctcgaa cggcgctaca aggaaacgga ctcgttgccc gtgcgcgaag agctggcgaa
    16021 atatctcggc agccagccct gccccgactg caacggcacg cgactcaatc gcacggcgcg
    16081 caacgtcttc gtgggcagtc acagcctgcc cgagattgcg catctcaccg tcggcgacgc
    16141 gaaaaagatg ttcagcacgt ttgatgtcgc gggctggcgc gcggaagtcg ccgacaagat
    16201 cgtcaaggaa gtcggcgacc gactcaagtt cctggtggac gtgggcctcg actacctcac
    16261 gctggatcgc agcgccgaat cgctgtccgg tggcgaagcg cagcgcattc gcctcgcgag
    16321 ccaggttggc tcgggcctga ccggcgtcat gtacattctc gacgaaccat cgatcggcct
    16381 gcatcaacgc gacaaccagc gcttgttggc cacgctcaaa cacctgcgcg atctcggcaa
    16441 cacggtgatg gtggtcgaac acgatgaaga agcgatccgc gctgcggacg aggtgctcga
    16501 catcgggcct ggcgccggcg tgcatggagg caagatcgtg gcgcagggca cgcctgccga
    16561 catcgagaaa caccccgatt cactgacggg caaatatctt tcgggccgcg tgaagatcgc
    16621 gctgcccggc ctgcgcacgc ctggagaccc caggcgtgtc atcaaggtga taggcgccac
    16681 cggaaacaac ctcaagaagg tggacgcgga atttccgctg ggactgttca cctgcgtcac
    16741 cggcgtgtcg ggctccggaa aatccacgct ggtcaacgac acgttgttca cgcatgcgaa
    16801 ccacaaactc aatgggaccg tgatggacat ggctccctgc caggagatca agggactgga
    16861 ggaaatcgat cggatcatcg acatcgacca gagccccatc ggccgcacgc cgcgctccaa
    16921 cccggccacc tacaccggct tgtttggccc gttgcgcgag ttgtttgccg aggtgccgga
    16981 gtcgcggact cgcggttacg acgccgggcg gttcagcttc aacgtgaaag gaggccgctg
    17041 cgaggcctgc cagggtgacg gactcatcaa ggtggaaatg catttcctgc ccgatgtgta
    17101 cgtgccctgc gacgtgtgcc agggaaaacg ctacaaccgc gaaaccctgg atatccgctg
    17161 gcgcggcaag aatatccacg aagtgctcga catgaacgtc gaggacgcac tgcggctgtt
    17221 ccagaacgtg cccggtgtgg gatcgcggct acagacgttg atggacgtag gcctgtccta
    17281 cctgcaactt gggcaaagcg cgaccacgct ctcgggcggt gaggcgcagc gtgtgaagct
    17341 ggcgcgtgaa ctcgccaagc gggccaccgg gcgcactcta tatatattgg acgagcccac
    17401 caccggcctg cacttccacg acgtggccca gcttctcgat gtgctgcacc ggctccgtga
    17461 tgaaggaaac acggtcgtgg tcatcgagca caatctcgac gtgatcaaga ccgcggactg
    17521 gatcatcgat ctcggcccgg aaggcggcgc gggcggcggc acgatcctgg tgacgggaac
    17581 tcccgaggaa gtcgcccgaa acaaggattc ctacacagcg cggtatctgc agcccctgct
    17641 ggtcccttag tgccgggcct ggcgccaccg gggtaacacg gcccgtcccg cctcgatgta
    17701 ggcgggacgg acctcttcac cgtcgatgat tccacaacta acaaagttgt gacgcgcgcg
    17761 cgtttccgga tggttcgcgc gcctatgtgc gatagcgtac agcgattccc cgcctgatta
    17821 cccaaactcc cgccacgcat atctgcgggc gcacatgcgc gcctatctac ttccgatgta
    17881 aagggacata catgaacgac ttcaattctt ttcgccgcgg tctatcgtgg tgcgcgacag
    17941 cgctgctcgc cactctggcc gcgggatgta ccggggactc gatcctcggc gccggcggcc
    18001 gcgtcgcaac ggcgcccatc gcgcccgtgg tcaccgcggt gtcgcctctc gacaatgcca
    18061 tcggcgtggc ggtgggcagc accgtggcca tcactttcga caaggcgatg gtagcgggga
    18121 cgacctttac ggtgacctgc gcggcgccct gcgtgaatcc caacggtacg gtcgctctca
    18181 gcgccggcgg caccgtcgcc acgtatacgt tggcagcgac aacctcgttc gcgcccctga
    18241 cgctctacac cgtgacagcc aacgcgccca tgagcgcggg cggccttccg ctggttacgc
    18301 ccttcgtatc gcacttcatt acggcgggca ccacggcgga cacgaaccgg cctcgcgtgt
    18361 tgaacacgtc gccgctgact tcgagtcccg gcccgacgct tgccgtcgcg acgaacgcct
    18421 cggtagccgc ggtgttcagc aaggacatga accaggcttc catcgaagcc actgcgcctg
    18481 ccacgttcac agtgacgtgc gtgactccct gcgtgtcgcc aaccggcgtg gtgagctact
    18541 cggtgggtag caggactgcg gtcttcatcc cgtcggcgcc gctcacgacg gccacgacgt
    18601 atacggcgac catcagcact gcggccatgg atctcgccgg caacgcgctc gccggcaacc
    18661 aggctgcgct gcccgcggcg agtgcctaca gctggacctt caccacggcc gctgcggccg
    18721 ctaccggcgc aatcaccgtg ttatcgacga gcccgctcgc cgcggcgacc ggggtctgcc
    18781 ccagcgcgac cgtcaatgcg acattcacgg tgccttccgg tctgcgcctg aatccgctca
    18841 ccgtgaacgc gaccaatttc accgtcgtcg ggcccgcacc cggtctcgta ccggtgaccg
    18901 cggcaaccat cagtgtcgac agcgccaccg ggcgaatcgc gactttcaca ccggcggcag
    18961 cgcttgcggc caataccagc tactcggcca cgctgatcgg cggtgccacg ggcgtcaagg
    19021 acctcgcgat ccccgcgaac gcgatggcgg caaattcgac ctggacattc accaccggcg
    19081 ccgccacctg cgttacgcca ccggccatcc cgctcaactc ggcggcggta ttcggtacgt
    19141 tcggcggcac cgccggcatg accaactccg gaatcctgac cgtggtcaac ggcaacatcg
    19201 gaacgatcgc gacgggcact tcgtcgatca ccgggttcca tgacaccgcg ggtgacatct
    19261 acaccgaatc gatcggcgcg aatgccggcg cggtgaacgg gactatctac acctgcacaa
    19321 actccacgac gggcccgacc tccgcaggtc cgaacgcggt caagtgcgcc gtcgccacgc
    19381 aggcgcggct cgatgcgcaa gcggcgtatc tgcaactggc cggcctcgca ccgggcgcga
    19441 atccgggcgc caatctcggt
//
LOCUS       DASQJS010000173        17458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3241428, whole genome shotgun sequence.
ACCESSION   DASQJS010000173 DASQJS010000000
VERSION     DASQJS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17458
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3241428"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VEW08_07295"
     CDS             <1..290
                     /locus_tag="VEW08_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40576.1"
                     /translation="ISVGRSHDEEPLSLVLPQREVRELAALLGFASARVRSSSPGAGS
                     DAVLALTDALSRVIAKYEAAVREDVLTVRETETQREALAEIARLARELDKY"
     gene            986..1327
                     /locus_tag="VEW08_07300"
     CDS             986..1327
                     /locus_tag="VEW08_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HYJ40577.1"
                     /translation="MAKKVNHQPVNPFHPGEVLLEEFLLPGNITQTEFAERLGWTRAR
                     LNELIKGKRGITAEAALDLAEALGTSPRLWMNLQATFDLAAAEKNAQLEAFKEAQDGA
                     AHVLMPFDIST"
     gene            complement(1373..3124)
                     /locus_tag="VEW08_07305"
     CDS             complement(1373..3124)
                     /locus_tag="VEW08_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYJ40578.1"
                     /translation="MTAGAKAALRTPAAEVAEAQPLRARDRLIFDVAPHPIFIADEAG
                     NTLAVNPAFVELLGYTAEELPDREQRMLKLVPDAMERARLTELWMAGVRVFKATGSQP
                     EPLVARVTCKDGGERTVEIRTSLAANESVTILTDITEALRGELDRKRIRAERDDLRAE
                     LGLRSDVLPIALVIADPTDELTTREWNPAAERIFGYSRAEMLGATPYDSIIPAEGHGY
                     VREVIRHVVAGSATTTVVAQNRTKDGRLIWCEWSSTIVRDSVGAPAHVISTVQDVTER
                     LLAEERKRLWTSVLEQSGEGIMICDTKRKILLVNAAFERLTGFSAAEAVGQSPAILRS
                     GRQSAAFYSQMWETLASVGYWSGEMWNRRKGGELYVEWLSISAVRDNQGAISHYVGIF
                     ADISERKAAADRILHFAQYDLLTELPNRVLLLDRLEQVIASSRRESRHAAVLFLDLDR
                     FKEVNDSMGHEVGDLLLQSVARRISGCVRESDTVARMGGDEFVVLLPAIGGSDGAANV
                     ARQLLDAVCAPQSLNGHEVSISASIGIAIFPDDGISTEDLLRNADAAMYHSKKNGRNA
                     FTFYTTELDLRVLEQPT"
     gene            complement(3121..4890)
                     /locus_tag="VEW08_07310"
     CDS             complement(3121..4890)
                     /locus_tag="VEW08_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYJ40579.1"
                     /translation="MTPATTATLSPRHASGKPSENRAAATNRAELRAALALPLRVHRE
                     PLGTLLADAGSLSDTGVNRALALQEASPGERLGDLLLSSGEIGEDDLYRALADQMGVL
                     YVRLGDFDADPAALAALAPEHARAHRLLPLMFERGRLIVATDDPSDSAALNALKFSTQ
                     YPVEAVLASPEDLDVAIATRYPAIGSETLDLKVDHAPAAPPIERLALDKPIVRLLNNL
                     LIDAVRRRASDVHLRAREHKAEVLYRIDGSLVAVGEFKRTVLPSVVARIKVLAGMNVA
                     EHRVPLDGAIHFKAARGSVDMRVSSIPTIYGENVVIRILDPSAGLRRLSDVGFTAGDE
                     KRMRSLIDRNQGLVLVTGPTGSGKTTTLYAALQELNNGEFHIVTVEDPVEYRLDGVVQ
                     IQVQPGIDFTFGKALRHILRHDPDIILLGEIRDAETAKIAVESALTGHLVLSTLHTNS
                     AAQAVTRLIEIGIPPYLVNATLAGVLAQRLVRRNCDRCKAVETVSVEVRATLGVAPSE
                     QFYCGKGCDECSGTGYRGRIAVYELLELSPAIRLLVRSGEPHERIEAQAVKEGMVRLT
                     AQALELARTGVISLTEVFRARLE"
     gene            5145..5432
                     /locus_tag="VEW08_07315"
     CDS             5145..5432
                     /locus_tag="VEW08_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40580.1"
                     /translation="MNIVDIAIAPGQHVSKTERHRMQAHALWEISASKYMLGGNVDAM
                     ARQITEMAAQATGVARANVWLFNDAEDELRCIDLYEARASRHSSGSVLREH"
     gene            5436..7391
                     /locus_tag="VEW08_07320"
     CDS             5436..7391
                     /locus_tag="VEW08_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYJ40581.1"
                     /translation="MNEFRALKLAPYVDANEPFTDPRTRGYVEGYIKPLGITSMLDTV
                     VAVSGKHLGLLCLEHVGQPHIWQPDEIAFAARLADKLAVTLALRLARDAEAASRAVAR
                     LAHESPNSVMRVRRDGQVLYANPASRDLQALFGAIVGEKANRRCLDIIDECISRGASH
                     EVELAHEERVYSVVASPILAEGYVNIYGTDISARKVAEVALRESRDLLLSVVENVPAR
                     VFWKDHDLRFLGCNSLFARDAGLASPQDLIGKNDFQMPWSAQAERYRADDRGVIESGV
                     PKLGIEEPQSSPGGRTVWVRTSKVPLRGPDATHVGVLGMYEDVTDRKAADDELRKSEE
                     RFRGYFELGLLGMAITMPDRRFSEVNGKLCEMLGYSRDELISMNWASLTHPEDLADDV
                     ARFEQLLSGATEGYSLDKRYIRKDGSTVYCTLSVSCVRRVDLTVEYIVALIQDITERK
                     LAEGTLHGALVATVEAIAATVEARDPYTAGHQRGVAVLAEAIAREMGLSAAVADGVRF
                     GALLHDLGKVQVPAEILSKPTKLSKPEFELIKTHPQAGYDIVKGIKFPWPVAEMILQH
                     HERIDGSGYPQGLKGDQITLGARILAVADVMEAMASHRPYRAGRGIDAALEELQGKRG
                     RWFDPAAVDACVRLFREKGFRLEKNLI"
     gene            complement(7590..8327)
                     /locus_tag="VEW08_07325"
     CDS             complement(7590..8327)
                     /locus_tag="VEW08_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40582.1"
                     /translation="MYAGTYDRPFIVDNGPRRFLHFDFGAIQSVMELSNPVRLALAYT
                     RKMMAFLLFNRAPKRILLLGLGGGSLAKFCYANLPTASLTAVEVNQDVIALRDEFSVP
                     EDDHRFRVINADGASYVSVLTHYKDVILADACDRAGIAAELGSVEFYRRARQHLSSDG
                     VFVANICGDRFSTATQLAKIREAFEDELLSLQVQTDGNIIVFGFKKRRPDRHWERIEA
                     GAADLKRRFHLDFPRYAQSIAVDSKLR"
     gene            complement(8438..9076)
                     /locus_tag="VEW08_07330"
     CDS             complement(8438..9076)
                     /locus_tag="VEW08_07330"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HYJ40583.1"
                     /translation="MSSLSKRYRLARPDPGEFMPPNLLTFGAAPFGHPQQARLTAWLG
                     EAGWPRGHMEIAELEGYLVALIAWPVGISSGAWLPRIWGHPGWKVPTKIATRSQYDEF
                     VALIVGFMQDLDRELSLQPPRFESSVLRGLREREHAETLHGWGRGFMTALTLGSQGLK
                     GRSASAGAAVHTIATSTASSAPFRSRTVEDIESAVLSLIEQRSSRGPLGPLG"
     gene            complement(9076..9282)
                     /locus_tag="VEW08_07335"
     CDS             complement(9076..9282)
                     /locus_tag="VEW08_07335"
                     /inference="COORDINATES: protein motif:HMM:NF039406.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40584.1"
                     /translation="MALEERTARLTILIDPRKKAVFERLCAEEDSTPSQVVRRLMRAY
                     IESRTGKPWSPEAEGKSLSRRKTA"
     gene            9385..11391
                     /gene="hyfB"
                     /locus_tag="VEW08_07340"
     CDS             9385..11391
                     /gene="hyfB"
                     /locus_tag="VEW08_07340"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HYJ40585.1"
                     /translation="MAPGSFSPLIACISLLAAWGVIGVLGLLRPSSLALSRRVLFPLG
                     ALAGLGLAVAALAGIAVPAERFVLPIGLPDLPMHLRRDALSSVFLFLLGAASAGISIF
                     GAGYFRAGEGTAPGLLCLQYHLFLASMGFVLLADDAYSFMVSWESMALSSYFLVTTQH
                     RIPEIRRAGFLYLLIAHLGAIAILLSFGVMQGGSWQFTFDAMRSATLTPTWAAVAFSL
                     ALFGFGAKAGLVPLHVWLPEAHPAAPSPVSALLSGVMLKTALYGMLRVTFDLLDHPVW
                     WWGLIPLVLGLFTAIYGVVFAAAQTDMKRLLAFSSIENIGILFTGVGLAIVFLGVGMS
                     TLAALALVAVLYHAINHAFMKSLLFLGTGSVLHATGERNLGRLGGLIHRMPWVAWLTL
                     VGALAIAGLPPLNGFVSEWLLLQAFLSAHEVPRSFVNMLLPLGAAVIALAAALSAYVM
                     VKFFGVVFLGQPREAGLAKAQEAGWLERAGLLWLAAGCVALGLMPTQVIALLSLVPEQ
                     LGIVDALPTTSTWWHLVPVAGRQAAYAPLVFLLAIIATIVITVALVSMFYHRRIRRSA
                     AWDCGFARLDARMQDTAEGFGQPIRHIFLPFFEMQRELPTPFDRAPQYRVVIGDRFWR
                     AIYLRLARWVQRIAEAVAWLQQGRIATYLLYSFVTLLVLLALVL"
     gene            11388..12341
                     /locus_tag="VEW08_07345"
     CDS             11388..12341
                     /locus_tag="VEW08_07345"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HYJ40586.1"
                     /translation="MTSLHWVTQVLEVLAALVAAPLFVGWINQCRAWLQNKSAPPLLQ
                     PYRGIRKLFHKDAVLATHASPLFRIAPYVVFGAMVCAAAVIPSLGTRLPFSDAADAIA
                     LVGLFATARVFMALAAMDVGTAFGSLGARREMLVGFLAEPALLMVIFLASLIAASTSL
                     PAISEGLATRTLALYPSLAFTGAAFLLVLLAENARIPVDNPATHLELTMIHEAMLLEY
                     SARHLALMEWAAALKLFNYACIGFALFLPWGSATGDSPSPVTLLASIPILGLKLLAAG
                     AVLALIETVSAKLRIFRAPEFLATAFLFAVLGLLVHLLLGS"
     gene            12346..13014
                     /locus_tag="VEW08_07350"
     CDS             12346..13014
                     /locus_tag="VEW08_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40587.1"
                     /translation="MSNLSLDLQLLNTFAALLLLSSFAMLSQRRVVTMVNLLAWQGVL
                     LGLATLLLAWRTGNSHLYVSAAMTFGLKALFLPWLLHRLIRRLGVYWDSEPLLNIAAT
                     MLVGLLIVIFAFGLAQPIAALATTSTRSSIGIAVAVVLLAFLTMITRRKAMSQVVGFL
                     SMENGLFFGAMSATYGMPMIIELGVALDVLVAMLVLGVFFFQIREQFDSLDLHHLESL
                     KEEK"
     gene            13016..14473
                     /locus_tag="VEW08_07355"
     CDS             13016..14473
                     /locus_tag="VEW08_07355"
                     /inference="COORDINATES: protein motif:HMM:NF005045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HYJ40588.1"
                     /translation="MEFLFLLTTPVLGALFLAFFGARRWAPEANAAFSLGTFVAACAL
                     TARVIGEGNLSAAREQFFIDPFNVFLVALTAFVSLTTSLFSRPYMRIEREHGRVRPAQ
                     LRLYHSMYQVFIAAMLIALTTNNMGLMWVAMEAATLSTVLLVTLYRTPASLEAGWKYF
                     ILCGVGIAQALFGTILLYFAAEKVLGAEGMSALLWTHLNEVKGQLEPTVLSLAFVFLL
                     VGYGTKVGLAPLHNWLPDAHAEGPTPISAVLSGLLLNVAIYAVVRCKVLVEGSLHSPL
                     PSRMLMGFGLVSMVLAAFFLWRQRDIKRLFAYSSIEHMGIITFAFGMGGAVANFAALL
                     HMTVHSLTKSAIFFAVGHAAQKAGTQVMDDIRGLITLSPTVGWGLMIGTLAILGLPPF
                     GVFASEFLILTTAMREQPWATPILLMALGVAFAAIFRRVQPMVFGETTARRLPHPPAL
                     LPVFVHLGLVLVLGLFIPSYLADWFRLAAKLIAGA"
     gene            14475..16004
                     /locus_tag="VEW08_07360"
     CDS             14475..16004
                     /locus_tag="VEW08_07360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYJ40589.1"
                     /translation="MELQNWIDQSTPLPGAPGLRQLDVTPDTWRACSMDVAASGGRLF
                     SLWADCAGEVTVFAALVAEPVVLLLVLELPAASTYPSLADIFPAAARMERAVADLCGV
                     SARDTDTRPWLRHVAWPANYFPLAGRSPSGVSNQSLIDDYPFVRVAGDGVHEIAVGPV
                     HAGIIEPGHFRFSVVGEKVLRLEERLGYTHKGVEQRFTQLQLPDGHRLAARVSGDSAV
                     AYSWAYCQALEGMAQTSIPARAAFLRALALECERIANHLGDLGALGNDAGFAFGLAQF
                     SRLKESWLRANESAFGQRYLMDFIVPGGTQGDPPATALAGLVDCAGMIAGKAALFRTI
                     YDEHAGVRDRFAGAGIVSPQLAAQLGLTGFAGRASGQAHDLRIDLPCEPYARLAVRKM
                     TSTEGDVFARVAVRFDELQESLRLVEQIVRQLPDDGHRTIVATPPDGSLGIGLIEGWR
                     GPVMMALTAGPAGQIRRCHPHDPSWQNWPVLEHAVIGNIVPDFPLINKSFNLSYSGHD
                     L"
     gene            16007..16522
                     /locus_tag="VEW08_07365"
     CDS             16007..16522
                     /locus_tag="VEW08_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40590.1"
                     /translation="MLKTLHQICRAGIVTESPPAIEEALRLGDTLQDRIRAILGRALC
                     IRHVDAGSCNGCELEIHALNNPVYNIEGLGIKFVASPRHADLLLVTGPVSRSMEAALR
                     RTYDATPEPKLVVAIGDCGCTGGVFGENYASVGRVGNVIPVNVAVPGCPPSPNRILAG
                     ILTAISPPAAK"
     gene            16715..>17458
                     /locus_tag="VEW08_07370"
     CDS             16715..>17458
                     /locus_tag="VEW08_07370"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HYJ40591.1"
                     /translation="MHFTNEATQSAMLFSDPDALISQYTRKMMAFLLFNPNPKRIVMI
                     GLGGGSLAKFCYRHLSRSHITVVEINEDVIALREEFCIPKDDDRFRVVHDDGARYVER
                     LDEKVDVLLIDAFDADGIALSLAESDFYSCAARQLTQSGMLVMNFWGPCDRYVDNLVQ
                     ARAAFGDSLLLVPVSGDVNVLLFAFKQAPPHSITDELERLAKRLQMRLLLDFPRYLRR
                     ICQGITLADSQTSTELQIAAAFCLPAHTGG"
BASE COUNT         2994 a         5347 c         5495 g         3622 t
ORIGIN      
        1 cgattagcgt tggtcgctcg cacgacgagg agccgttgtc gctcgtcctc ccgcagcgcg
       61 aagtgcgtga gcttgctgcg cttcttggct tcgctagcgc tcgggtgcgt tcttcgagtc
      121 ccggggccgg aagcgacgcg gtgcttgcgc ttaccgatgc gttgtccaga gtcatcgcga
      181 agtacgaggc ggccgtgaga gaagatgtgc tcaccgtccg cgaaacagaa acgcaacgcg
      241 aggcgctagc ggaaattgca cgactggcgc gagaactcga caagtactga tgttcgatcg
      301 tggcgaccac caccgtcttg agctggaaca cccctgttcc agagggtgtt ccagcaggac
      361 caaaacgttc caaagtgttc cagacggtgg cgcctaagtc cttgtctcca ttgatgcgct
      421 tggtgatcga atctctccgg gcgcgccaat ttctcttacc gtgacaatgt cttgccgcgg
      481 ttcacttctc ccactcgtcg ccgtaaccag gtggttgcgg gggaattgcg tgctcctggt
      541 gttttgccag gtagtcgcgc ctgttccgta ccctgttgtg agactcgcgc gccgccggaa
      601 cccgttacat cgcccgccat cacggcgacg atccgatcgt cgaaccggta gctctcgtcg
      661 ccagcgatgt tgggttattg gtgaagcgct actacgccta tctgcgcaat aacctagaaa
      721 ccgcgcgagg cccaaccccg gatctggcga aggccgtggc ggcgccgacc gacaactggc
      781 cgcctccccg gcccgatcgc cggcatgcga aatgtggaat tgctcactcc tgcgaagagt
      841 ccgggttgcc caagttaggg cggtggaagg gtttctactc gattcgaatc aatgatcact
      901 ggcgtgtcgt tttccgattc gaaagcggca atgcctcgga tgttcgagtt gtcgactatc
      961 actaggaaga gggctaggag gagatatggc caagaaagta aaccatcagc cggtgaatcc
     1021 gtttcatcct ggcgaagttc tgctcgaaga atttctgctg cccggaaaca tcacgcagac
     1081 agagtttgcg gagcgcctcg gatggacgcg cgcgcgtctc aatgagctga tcaaggggaa
     1141 gcgtggcatt accgcggaag cggcactcga tttggccgag gcgctcggta cgtcgccgcg
     1201 actgtggatg aatctgcagg caacttttga tctcgcggct gcggaaaaaa acgcgcagct
     1261 tgaagcattc aaagaagctc aggacggggc cgcgcatgtc ctcatgccgt tcgatatatc
     1321 cacttgacat gggtcggatc gttgttggtc agaccctcca ttccattcca attcaagtcg
     1381 gctgctccaa tacgcgtaga tcgagttccg ttgtgtagaa cgtgaacgca ttgcggccgt
     1441 tcttcttcga gtgatacatc gccgcgtcgg cgttcctgag cagatcttcc gtcgaaatcc
     1501 catcatccgg aaagatggcg attccaatgc tcgccgagat gctgacttca tgtccgttga
     1561 gcgactgtgg tgcgcaaaca gcgtcgagga gttgccgggc gacattcgcg gcgccgtcag
     1621 agccgccaat cgccggcaac aacacgacga actcgtcgcc gcccattcgt gccacggtgt
     1681 ccgattcacg cacgcatccg gagatgcgcc gtgccaccga ctgtaacagc aggtcgccaa
     1741 cttcatgtcc catggagtcg ttgacttcct tgaaccggtc gagatccaga aacaagaccg
     1801 ccgcgtgccg ggactcccgt cgtgaagagg cgatcacttg ctcgaggcga tcgagcaaca
     1861 acacccggtt cggcagctcg gtcagcagat cgtattgcgc gaaatgcagg atgcggtcgg
     1921 ccgcggcctt gcgctcgctg atgtcggcaa agatgccaac gtagtggctg atcgcgccct
     1981 ggttgtcgcg aaccgcactg atcgacagcc actcgacgta cagctctccg cccttgcggc
     2041 gattccacat ctcgccgctc cagtagccca cggatgccag cgtctcccac atttgtgaat
     2101 agaacgcggc gctttgccgg cccgatcgca ggattgcggg agattggccg actgcctccg
     2161 cggcggaaaa acccgtcagc cgctcgaagg ccgcgtttac cagcaggatc ttccgcttcg
     2221 tatcgcagat catgatgcct tcgccgctct gctcgagcac cgaagtccac aatcgcttgc
     2281 gttcctccgc gagcagtcgc tcggtcacgt cttgaaccgt ggagatgaca tgcgctggcg
     2341 caccgactga gtcgcgtacg atggtgctcg accattcgca ccagatcagg cgaccgtcct
     2401 tggtccgatt ttgcgcgacg acggtggtgg tagcggatcc cgcgaccacg tgacgaatga
     2461 cttcacgtac gtagccgtga ccctcggcgg gaattatgga gtcgtacggt gttgcgccaa
     2521 gcatttccgc acggctgtag ccaaatattc tttccgccgc ggggttccac tcgcgcgtgg
     2581 tcaattcatc cgtagggtcg gcgatcacga gcgcgatcgg caacacatcg ctgcgcaagc
     2641 cgagttcggc gcgcaggtcg tcgcgctccg cgcggatgcg cttgcgatcg agttctccgc
     2701 gcagggcttc ggtaatgtcg gtcaggatcg tgaccgactc gttcgcggcg aggctggtgc
     2761 ggatttccac cgtgcgttcc ccgccgtcct tgcaggtcac tcgcgcgaca aggggttcgg
     2821 gttggctgcc ggttgccttg aagacgcgaa cgcctgccat ccacaactca gtcagtcgtg
     2881 cgcgctccat agcgtcgggt accagcttga gcatccgctg ctcgcgatcc ggaagttcct
     2941 cggccgtata accaagcagt tcgacaaagg cgggattcac cgccagggta ttacccgctt
     3001 cgtcggcaat gaaaattgga tgcggtgcga catcgaatat caggcggtct ctcgcgcgca
     3061 aaggctgcgc ctcagcgact tcggccgcgg gcgttcgcag ggctgccttc gcgcccgctg
     3121 tcattcgagc ctggctcgaa agacttcggt gagggaaatc acaccggtgc gtgcgagttc
     3181 gagtgcctgc gccgtgagcc tcaccatgcc ttccttcacg gcctgcgcct cgatgcgctc
     3241 gtgaggttca ccgctgcgca ccagcaggcg aatcgccgga gacagttcca gcagctcgta
     3301 taccgcgatt cggccgcgat agccggttcc cgaacattca tcgcagccct tgccacaata
     3361 gaattgttcg gacggcgcaa ccccgagcgt cgcccgcacc tcgacggaaa cagtttccac
     3421 cgccttgcag cgatcacagt tgcgcctgac tagtcgctgt gcgagtactc cggcgagggt
     3481 cgcattgacg aggtacggcg gaataccgat ctcgatgagt cgcgtcaccg cttgcgcggc
     3541 gctgttcgtg tgcagcgtcg ataacaccag gtgtccggtg agtgcgcttt ccacggcgat
     3601 ctttgcggtc tcggcgtcgc ggatttcgcc gagcaggatg atgtccggat cgtgccgcag
     3661 gatgtgccgc aacgccttcc caaaggtgaa gtcgattccg ggctgcacct ggatctgcac
     3721 cactccgtcg agccggtact cgaccggatc ctcaacggtc acgatgtgaa attcgccgtt
     3781 gttgagttcc tgaagcgctg cgtacagtgt cgttgtcttt cccgatcccg tcgggccggt
     3841 gacgagcaca agtccctggt tgcggtcgat gagtgagcgc atgcgctttt cgtcgcctgc
     3901 agtgaagccc acgtcggaca ggcgtcgcag tccggcgctg gggtcgagaa ttcgtatcac
     3961 gacgttttcg ccgtaaatgg tggggatcga ggagacgcgc atgtcgacgg aaccgcgcgc
     4021 ggccttgaag tggatagcgc catcgagggg aacgcggtgc tcggcgacat tcatgccggc
     4081 gagcaccttg atgcgtgcga ccaccgatgg cagcaccgtg cgcttgaact cgccgacagc
     4141 tacgagcgag ccgtcgatgc gatagagcac ctccgccttg tgttcgcgcg cgcgtagatg
     4201 gacatccgag gcgcggcgcc gtacggcgtc gatcaacagg ttattgagga ggcgcacgat
     4261 cggcttgtcg agcgccaagc gctcgatggg cggcgccgcc ggagcgtggt ccaccttgag
     4321 atcgagagtt tcgctcccga tcgccggata gcgggtcgcg attgcgacgt cgagatcttc
     4381 cgggctcgcg aggaccgctt cgaccgggta ttgagtcgag aatttcagtg cgttcagcgc
     4441 ggccgagtcg gagggatcgt ccgtcgccac gatcagtcgt ccgcgttcga acatcaatgg
     4501 cagcaagcgg tgcgcacgcg catgctcggg cgccagtgcc gccaacgccg ccgggtctgc
     4561 gtcgaaatct cccaggcgca catacagcac acccatctga tcggcgagtg cccgatacag
     4621 gtcgtcttcg ccgatctcgc cggatgacag gagcaggtcg ccgagccgct cgccggggct
     4681 cgcttcctgc agggccagcg cgcgattcac gccggtgtcg ctcaagctgc ccgcatccgc
     4741 cagcaatgtg cccagaggtt ctcgatggac gcgcagcggc aatgcgagcg ccgcgcgcag
     4801 ttcggcacgg ttggtggccg ctgcacgatt ttccgacggc ttgccgctcg catggcgcgg
     4861 cgagagggtg gcagtggtgg caggggtcat gtcaataatc cttcgctgga gtgggacgtt
     4921 gaccaagtat cggccggcaa gtggcatgca gcgcccattc gggtcgcatt tcggtgaact
     4981 acgtacatac ctgcggagat cacggatctc cgcaccgcgc agagatgcgc ctgttgagcc
     5041 ttgctcccgt gttcccttac tacttttcca ccgcgacctg cttcctcgac ttgtctgcct
     5101 gcttgcggat aattttcacg agcacgcatt gagaatcgga cgccatgaac atcgtcgaca
     5161 tcgccatcgc ccccggccag cacgtcagca agactgaacg ccaccgcatg caggctcacg
     5221 ccttatggga gatatcggcg tcgaaataca tgctcggagg aaatgtcgat gcgatggcgc
     5281 gccagatcac ggaaatggcc gcgcaggcaa cgggcgtggc gcgcgccaac gtctggttgt
     5341 tcaacgacgc ggaagacgaa ctacgctgca tcgatctgta cgaggcgcgg gcaagccggc
     5401 attcgtccgg ctcagtgctg cgcgagcact agttcgtgaa tgaattccgg gcgctgaaac
     5461 ttgcgcccta tgtcgacgcg aacgagccat tcacggatcc acgtacgcgc ggttacgtcg
     5521 agggttacat caagccgctg ggcatcactt ccatgctgga taccgtcgtc gcggtttcgg
     5581 gcaaacacct cggtctgttg tgcctggaac atgtcggcca gccccatatc tggcagcccg
     5641 atgagatcgc cttcgctgcg cgcctcgccg acaagctggc ggtgacgctg gcactgcgtc
     5701 tcgcccgcga cgcggaagcg gcatcgcgcg cggtcgcacg cctggcccac gagagtccga
     5761 actcggtgat gcgagtgcgt cgcgacgggc aggtgctata tgccaatccg gcgagtcgcg
     5821 acctgcaggc gctgttcggc gccatcgtcg gcgaaaaggc caatcgacga tgcctcgaca
     5881 tcatcgacga atgcatttct cgcggcgcgt cgcacgaggt cgaactggcg cacgaagaac
     5941 gggtctactc ggtggtcgcc tcgccgatcc tggcggaagg ctatgtgaac atctacggca
     6001 ccgacatcag cgcccgcaaa gtggccgaag tcgcgctgcg tgaatcgcgg gatctcctcc
     6061 tttcagtcgt ggaaaatgtg cccgcgcgcg ttttctggaa ggaccatgac ctgcgcttcc
     6121 ttggctgtaa cagccttttc gcgcgcgatg cgggccttgc aagcccccag gatcttatcg
     6181 gcaagaacga cttccagatg ccctggagcg cgcaggctga gcgttatcgc gccgacgaca
     6241 ggggcgtcat cgaatccgga gtgccaaagc tcggtatcga ggaaccgcag tcatcgcccg
     6301 gcgggcgtac cgtctgggtg cgaacctcaa aagtaccgtt gcgcggtccg gacgcaacgc
     6361 atgtgggcgt cctcggcatg tacgaggacg tcaccgatcg caaggccgcg gatgatgagc
     6421 tgcgcaagag tgaggaacgt ttccgcggct atttcgagct cgggctgctc ggcatggcga
     6481 tcaccatgcc ggacaggcgc ttctcggagg tcaatggcaa gctctgcgag atgctcggtt
     6541 actcgcgcga cgaattgatc tccatgaatt gggcgtccct cacgcatccg gaggatctcg
     6601 ccgatgacgt cgcccgattc gagcagctgt tgtcgggcgc gacagaaggt tattcgctcg
     6661 acaagcgcta catccgcaaa gatggctcga ctgtctactg cacgctgtcg gtcagctgcg
     6721 tgcgccgggt ggacctcacc gtggaataca tcgtcgcgct gatccaggac atcacggagc
     6781 gaaaactggc agaaggcacg ctgcacggtg cgctggtcgc cacggtcgag gccatcgcgg
     6841 caaccgtcga ggcacgtgat ccctatacgg cgggccacca gcgcggagtt gcggtgctgg
     6901 ccgaagcgat tgcgcgggag atgggattgt ctgccgcagt ggcggacggc gtaaggttcg
     6961 gagccctgct tcacgacctg ggcaaagtac aggtcccggc ggagatcctg tcgaaaccga
     7021 ccaaactttc gaagcccgaa ttcgagctga tcaagacgca tccccaggcg ggctacgaca
     7081 tcgtcaaggg aatcaaattc ccctggcccg ttgccgagat gatcctccag caccacgagc
     7141 gaatcgacgg atcgggctat ccgcagggac tcaagggcga ccagataacc ctcggagcgc
     7201 gaattctcgc ggtcgcggac gtcatggagg cgatggcctc gcatcgcccg tatcgcgccg
     7261 ggcgcggaat cgatgcggcg ctggaagagc tccagggtaa gcgcggacgg tggttcgacc
     7321 cggccgcggt ggacgcctgt gtgcggctgt tccgcgagaa gggcttcagg ctggagaaaa
     7381 acctgatctg atgaggctca tcggcgatct gcgcgaacgg tcagcgtcgg ttcttcggcg
     7441 ctcgagtgag ggtcggccag gcgaacagtc gacggcacca gccgctggga gacgactccc
     7501 ttgaggttca tcagtcccga ggtgagtgcg ggctcagtaa gggcgtcagg tcggtcgtac
     7561 ctaattcacg agtctcgagc tgcgcgcgct catctcaact tagagtcgac ggcgatgctc
     7621 tgcgcgtatc tcgggaaatc gaggtggaat cgccgcttga gatcggccgc acccgcttcg
     7681 atccgctccc aatgccgatc gggacggcgc ttcttgaagc caaagacgat gatgttgccg
     7741 tctgtctgaa cctgcagcga caacagctcg tcttcaaagg cttcacgaat tttcgccaat
     7801 tgagtggcag tggagaatct gtcgccacag atgttggcga caaatacccc gtcagaagac
     7861 aagtgttggc gcgccctccg gtagaactcg actgacccga gttctgcggc gatgcctgcc
     7921 cggtcacagg cgtccgcgag gatcacgtcc ttgtagtgcg tcagtactga aacataggac
     7981 gcgccatcgg cgttgatcac gcgaaaacga tgatcgtcct cgggaacact gaattcatcg
     8041 cgtagtgcga tgacatcctg attcacctcg acggcggtga gcgatgcggt gggcaggttg
     8101 gcgtagcaga acttggcgag agatccgccg ccgaggccga gcagcaggat gcgctttgga
     8161 gcgcggttga agaggaggaa cgccatcatc ttgcgtgtat aggcgagggc cagccttacc
     8221 ggattgctca gctccatcac gctttgaatc gcgccgaaat cgaaatgcag aaatcgccgc
     8281 ggaccgttgt cgacgatgaa cggcctgtca taagtgcccg cgtacacccg ctctcgaagc
     8341 gtctcgtgac aactgtccgg tggctcgaga aagcgcaacc tgacgtttgc gcccgggaac
     8401 ggactctcga ttgccaggag ccgcgacgag cggcgtttca accgagcgga cccaggggcc
     8461 cgcgcgaaga tcgctgttca atgagcgaga gcacagcgct ctcgatgtct tcgactgtgc
     8521 gcgagcggaa cggggcggac gatgctgtgc tggtggcgat ggtgtggaca gcggcgccag
     8581 cactggcact gcgccccttg agcccctgtg agcccagcgt caacgccgtc atgaatccac
     8641 gtccccagcc gtgcagtgtt tcggcgtgtt cgcgttctcg cagaccgcgc agcaccgacg
     8701 attcgaatcg cggcggctga agagaaagct cgcgatcgag atcctgcatg aagccgacga
     8761 tcaacgcgac gaactcatcg tattgcgatc gcgttgcaat tttcgtcggc accttccagc
     8821 cggggtgacc ccagatgcgc ggcaaccatg cgccggagga aatacccacc ggccaggcaa
     8881 tcagtgccac tagatagccc tcgagctccg cgatctccat gtggccgcgc ggccagccgg
     8941 cttcgcccag ccaggccgtc aggcgcgctt gctgcggatg gccgaaaggc gcggcgccaa
     9001 acgtgagcag gttcggcggc atgaactcac ctggatcggg ccgcgccagg cgataccgtt
     9061 tggacaaact actcatcagg cggtttttcg gcgcgacaag ctcttgcctt ccgcttccgg
     9121 cgaccagggc ttcccggtgc gcgattcgat ataggcgcgc atgagtcgcc gcacaacctg
     9181 ggagggcgtc gagtcctctt cggcacagag acgttcgaac acggctttct tgcgcgggtc
     9241 gatcaggatt gtcaggcgcg cggtgcgttc ttccagggcc aaggagtttc tctcgtgagg
     9301 gggatgcgaa tcacattatc atactattca catttgatcg tcatcctgca acactaaggc
     9361 ggattcaacg taagttggag cgtggtggca cccgggtcgt tttctccttt gattgcatgt
     9421 atttccctgc tcgctgcctg gggagtcatc ggcgtgctcg ggcttctgcg accgtcgagc
     9481 ctggctcttt cgcgtcgtgt cctgtttcca ctgggcgcgc tcgcgggcct gggcctggcc
     9541 gtggcggccc ttgccggcat cgcggttccc gctgagcgct ttgttctacc catcggcttg
     9601 cccgacctgc cgatgcacct gcgacgcgat gcgctcagca gcgtgttcct gttcctgtta
     9661 ggtgcggcgt ccgccggcat ctccattttc ggtgcgggtt attttcgcgc gggtgagggg
     9721 accgctccgg gcctgctgtg cctgcaatac cacctgttcc ttgcaagcat gggttttgtg
     9781 ctgcttgcgg atgacgccta cagcttcatg gtgtcgtggg aatcaatggc gctgtcgtcc
     9841 tattttctcg taaccacgca gcatcgaata cccgaaatcc gtcgcgccgg attcctctac
     9901 ctgctcatcg ctcacctggg cgccatcgcc atcctgctgt ccttcggagt catgcagggc
     9961 gggagctggc aattcacatt cgacgccatg cgttcggcca cgctcacacc cacgtgggcc
    10021 gcggtcgctt tttcactggc cttgtttggc ttcggcgcga aagcgggatt ggtcccgttg
    10081 cacgtatggc tgccggaggc gcatccggcg gcgccgtcgc cggtgtctgc gcttctgagc
    10141 ggcgtgatgc tcaagacggc gttgtacgga atgctgcggg ttacgttcga cttgctggat
    10201 catccggtct ggtggtgggg cctcattccg ctcgtgctcg ggctgttcac ggcgatctat
    10261 ggcgttgtgt tcgcggccgc gcaaaccgac atgaagcgcc tgctggcgtt ttcatccatc
    10321 gagaacatcg gcattctgtt caccggcgtc ggcctcgcca tcgtctttct tggcgttgga
    10381 atgtcgacgc tcgcggcgct ggcgttggtg gcggtgctct atcacgcgat caaccacgca
    10441 ttcatgaaga gcctgctgtt ccttggaacg ggctcggtgt tgcacgcaac gggtgagcgc
    10501 aatcttggcc gcctgggcgg gctcattcac cgcatgccct gggtcgcctg gctgaccctg
    10561 gtcggcgcgc tggcgattgc ggggctgccg ccgctcaatg gctttgtttc cgaatggctg
    10621 ctgttgcagg cattcctctc ggcgcacgaa gtgccgcgct cgttcgtcaa catgttgctg
    10681 ccgcttggcg cggccgtcat tgcgctcgcg gcggcgctct cggcctacgt catggtcaaa
    10741 ttcttcggcg tggtgtttct gggccagccg cgcgaggctg ggctggccaa agcgcaagaa
    10801 gcgggatggc tcgagcgtgc gggactgttg tggcttgcgg cgggctgcgt ggcgcttggc
    10861 ctgatgccga cgcaggtgat cgcgctgctc tccctggtgc ccgagcagct gggcatcgtc
    10921 gatgccttgc cgacaacttc gacctggtgg catctcgttc ccgtcgccgg tcgccaggcc
    10981 gcttatgcgc cgctggtgtt cctgctggcc atcatcgcca cgattgtcat caccgtggcc
    11041 ctggtcagca tgttctatca ccgcaggatc cgtcggagcg cggcctggga ctgcgggttt
    11101 gcgcggctcg atgcgcgcat gcaggacacg gcggaaggct tcggccagcc gattcgccac
    11161 attttcctgc cgtttttcga gatgcagcgg gaactgccga ctcccttcga tcgcgcgccg
    11221 caataccgtg tcgtgatcgg tgatcgcttc tggcgcgcga tctatctacg gctcgcgcgg
    11281 tgggtgcagc ggattgcgga ggccgttgca tggctgcagc aggggcgcat cgccacctat
    11341 ctgctgtaca gcttcgtcac tctgctcgtg ttgctggcgc tggtgttatg acttcactgc
    11401 actgggtcac gcaggtcctc gaagtgctgg ccgcgctggt cgccgcaccg ctgttcgtgg
    11461 ggtggatcaa ccagtgccgt gcctggctgc agaacaagtc ggcgccgccg ctgctgcagc
    11521 cgtatcgcgg aatccgcaag ctgttccaca aggacgccgt gctcgccacg cacgcctcgc
    11581 cactgtttcg cattgcaccg tacgtggtat tcggcgccat ggtgtgcgcc gcggcggtga
    11641 ttccttcgct cggcactcgt ctgccgttct ccgacgcggc cgatgcgatc gcgctcgtcg
    11701 gcctgttcgc caccgcgcgc gttttcatgg cactcgcggc gatggatgtg ggcacggcat
    11761 tcggctcgct cggcgcccgg cgcgaaatgc tggtgggctt cctcgctgag cccgcgttgc
    11821 tcatggtgat cttcctggca tcactgatcg ccgccagcac ctcgctgccg gcgatttccg
    11881 agggattggc aacgcggact ctcgcgctgt atccgagtct ggcgtttacc ggggccgcat
    11941 ttctgctggt gctgctggcg gaaaacgcgc gcattccggt cgacaatccg gcgacccatc
    12001 tcgaactcac gatgattcac gaagcgatgc tgctcgagta ttcagccagg cacctggcgc
    12061 tgatggaatg ggccgcggcg ctcaagctgt tcaattacgc ctgcatcggt tttgcgctct
    12121 tccttccatg gggctccgct accggagact caccaagccc ggtgacactg ctggcgtcca
    12181 tcccgatcct gggcttgaag ctgctcgcgg ctggcgccgt gctggcgctc atcgaaaccg
    12241 tttcggcaaa actgcgcatc tttcgcgcgc cggagttcct cgccacagcc tttctgttcg
    12301 ctgtgttagg cctgctcgtc catttgctcc tggggtcatg aaaagatgag caacctgagc
    12361 ctcgatctgc aactgctgaa taccttcgcc gcgttgttgc tgctgtcttc attcgccatg
    12421 ttgtcgcagc gacgggtcgt gaccatggtc aatctgctgg cgtggcaagg tgtgctgctc
    12481 gggcttgcga cactgctgtt ggcctggcgc acgggcaata gtcacttgta tgtctcggcg
    12541 gcgatgacct ttggcctcaa ggcgctgttc ctgccgtggc tgctgcatcg attgatccgg
    12601 cggctcggcg tctattggga cagtgagccg ttgctgaaca ttgccgcaac gatgctggtg
    12661 gggttgctga tcgtgatctt cgcgttcggc ctggctcagc cgatcgccgc gctcgcgacc
    12721 acgtccacac gcagttcaat cggcatcgcg gtcgcggtcg tattgctcgc gttcctcacc
    12781 atgatcacgc ggcgcaaggc gatgtcacag gtggtgggat tcctgtcgat ggagaatgga
    12841 ttgttcttcg gcgccatgag cgccacgtat ggcatgccga tgatcatcga gctgggagtc
    12901 gcgctcgacg tgctggtcgc catgctggtg ctgggcgtgt tcttcttcca gatccgcgag
    12961 caattcgaca gtctcgatct gcatcacctc gagtcgctca aggaagagaa atagcgtgga
    13021 gttccttttc ttattgacca ccccggtcct tggcgcgctg tttctggcat ttttcggggc
    13081 gcggcgctgg gcgccggagg cgaatgcagc gttcagcctg ggcacgttcg tggccgcctg
    13141 cgcgctcacc gcgcgagtaa tcggcgaggg gaatctgagc gcggctcgcg aacagttctt
    13201 catcgatcct ttcaacgtgt tcctggttgc cctgacggcg ttcgtgagcc tcaccacctc
    13261 actgttctca cgaccctaca tgcgcatcga gcgtgagcac ggacgcgtca gacctgcgca
    13321 actgcgcctg tatcacagca tgtaccaggt gttcatcgcg gccatgctga tcgcgctgac
    13381 caccaacaac atggggctga tgtgggtggc gatggaggcg gcgacgctgt cgacggtgct
    13441 gttggtaacg ctttaccgca cgcccgcgag cctcgaagcg ggctggaagt atttcatcct
    13501 gtgcggcgtt ggaattgcac aggcactgtt cgggacgatc ctgttgtatt tcgccgccga
    13561 gaaagtgctg ggggcggaag gcatgagcgc gctgctctgg acacatctaa atgaggtcaa
    13621 gggtcagctg gaaccgaccg tcttgagcct ggcgttcgta tttctcctgg tcggttacgg
    13681 caccaaggtc ggtctcgcgc cgctgcataa ctggttgccc gacgcgcatg ccgaaggccc
    13741 cacgccgatt tccgcggtgc tctcgggact cctgttgaat gtcgccatct acgctgtcgt
    13801 gcgctgcaag gtgctggtcg aaggctcgct gcactcgccg ttgccttcgc gcatgttgat
    13861 ggggttcggg ctggtgtcca tggtcctggc ggcgttcttc ctctggcgcc agcgtgacat
    13921 caagcgtttg tttgcctatt cgtccatcga gcacatgggc atcatcacct tcgcgttcgg
    13981 catgggtggc gcggtggcaa atttcgccgc gctgctgcac atgacagtgc actcgctgac
    14041 gaagtccgca attttcttcg ccgtgggtca tgccgcgcag aaggccggta cccaggtcat
    14101 ggacgacatt cgcggattga tcacgctcag tccgaccgtg ggttggggat tgatgatcgg
    14161 gacgctggct attctcggcc tgccaccgtt tggcgtgttt gccagcgaat ttctgattct
    14221 caccacggcg atgcgcgagc aaccgtgggc cacgccgatc ctgttgatgg cgctgggcgt
    14281 cgcatttgcg gcgatcttcc gccgggtgca accgatggtc tttggcgaga ccaccgccag
    14341 gcgcttgccc catccgcccg cattgctgcc cgtgttcgtt cacctggggc tggtcctggt
    14401 gcttggattg ttcattcctt cctaccttgc tgactggttc cggctggcgg caaaactgat
    14461 tgcgggcgcc tgacatggag ctgcaaaact ggattgatca gtccacgccg ctgcccggag
    14521 cgcccgggtt gcggcaattg gatgtgactc cggatacctg gcgcgcctgc tcgatggatg
    14581 tggccgcgag cggcggccgc ttgttttcct tgtgggcgga ttgcgcgggc gaagtcacag
    14641 tgttcgccgc gctggtcgcc gaaccggttg tgttgcttct cgtgctcgaa ctgccggcgg
    14701 cgtcaaccta tccgagcctt gccgacattt tccccgccgc cgcgcgcatg gagcgcgccg
    14761 tcgcggatct ttgtggtgtc agcgcgcgtg acaccgacac caggccgtgg ttgcgccatg
    14821 tcgcgtggcc ggcaaactat ttcccgctgg cgggtcgttc gccgtctggc gtgtccaatc
    14881 aatccctcat cgacgactat ccattcgtcc gcgttgccgg cgatggcgta cacgagatcg
    14941 cggtcggccc cgtgcacgcg ggcatcatcg aaccggggca tttccgattc tcggtcgtcg
    15001 gggagaaggt gctcaggctc gaagagcgcc tgggctacac ccacaaaggc gttgagcagc
    15061 gattcaccca actgcaattg cccgacgggc atcgcctggc tgctcgagtc tctggcgatt
    15121 ctgccgtcgc gtattcgtgg gcttactgcc aggcgctcga agggatggcg cagacatcga
    15181 ttccggcccg cgccgccttc ttgagggcct tggcgctcga gtgcgaacgc atcgcgaatc
    15241 atctcggcga tctgggagcg ctgggtaatg acgccggttt cgcattcggg ctcgcgcagt
    15301 tttcgcggct caaggagagc tggctgcgtg cgaacgagtc ggctttcggg cagcgatacc
    15361 tgatggattt catcgtgccg ggcggtacgc aaggcgatcc gccggccact gcgctcgccg
    15421 gtcttgttga ctgcgccgga atgatcgccg ggaaagcggc cttgtttcgc accatctacg
    15481 acgaacacgc aggtgtgcgc gatcgttttg ccggcgcagg catcgtgtcg ccgcagctcg
    15541 cagcgcagct tggtctcacg ggttttgcgg ggcgcgcaag cgggcaggca cacgacctgc
    15601 gcatcgatct gccgtgcgaa ccatacgcaa ggctggcggt gcgcaaaatg acgagcaccg
    15661 aaggcgatgt gttcgcgcgt gtcgcggtgc gtttcgatga actgcaggaa tcgctgcggc
    15721 tcgtcgagca gatcgtcagg caactgccgg atgacggtca ccgcacgatt gtcgcgactc
    15781 cgccggacgg cagcctggga atcggcctga tcgaagggtg gcgcgggccc gtgatgatgg
    15841 cgctcacggc aggtcctgca ggccagatcc gtcgctgcca cccccacgac ccgtcctggc
    15901 agaactggcc ggtgctcgag cacgccgtca tcggcaacat cgtgccggat tttccgctca
    15961 tcaataagtc gttcaatctg tcctacagcg gacacgatct ctaaccatgt tgaagaccct
    16021 gcatcaaatc tgccgcgcgg gcatcgtcac cgaaagccca cccgccatcg aggaagcgct
    16081 gcgcctcggt gatacgttgc aggatcgcat tcgtgccatt ctcggacgcg cgctgtgtat
    16141 ccggcacgtg gatgccggat cctgcaatgg ctgcgagctc gaaatccatg ccctcaacaa
    16201 tcccgtgtac aacatcgagg gactgggcat caaattcgtc gccagcccgc gtcatgccga
    16261 cttgttgtta gtcacgggtc cggtctcgcg cagcatggaa gccgcattgc gtcggaccta
    16321 cgacgccaca ccggagccca agctcgtcgt cgcgatcggc gattgcggtt gcaccggcgg
    16381 agtctttggc gagaactacg ccagcgtcgg ccgggtcggc aacgtgattc cagtcaacgt
    16441 ggcggtgccg ggatgtcctc cgagcccgaa ccggatcctg gccgggatcc tgacggcgat
    16501 ttcaccgccg gcggcgaaat aggactcgca tcagcacatg tccaagtcaa caaaacccca
    16561 gtggctggaa ttcgagagtc cgtgggcttg ccaacccggc aaggtacgcg tgctccaatc
    16621 ggcagaagac gtcagccgcg acgaactcgc gaagcgagcc tggcgcgacg agcttgcgcg
    16681 gccatacatc ttcgagaccc tgttcgaacg gcgaatgcat ttcacgaacg aagccacgca
    16741 aagcgccatg ctgttcagcg accccgatgc gctgatttcc cagtacaccc gcaagatgat
    16801 ggcgttcctg ctgttcaatc caaatcccaa gcggattgtg atgattgggc tgggtggcgg
    16861 ttcgctggcc aagttctgtt atcgccatct gtctcgttcg cacatcacgg tcgtcgagat
    16921 caatgaagac gtcatagccc tgcgcgagga attttgcatt ccgaaggacg acgaccggtt
    16981 tcgcgtcgtt cacgatgacg gtgcccggta tgtcgaaagg ctcgacgaga aggtggatgt
    17041 actgttgatc gacgcctttg acgcagatgg aatcgcgtta tccctggcgg aatccgactt
    17101 ctattcctgc gctgcgcgcc agctcacgca aagcggaatg ctcgtgatga atttttgggg
    17161 gccgtgcgac cgctacgtcg ataacctcgt gcaagcgcga gctgcctttg gggacagcct
    17221 gctgctcgtt ccagtatcgg gcgacgtgaa tgtcttgttg tttgcgttca agcaggcgcc
    17281 gccgcactcg atcacggacg agctcgagag gctcgcgaag cggctacaga tgcgtcttct
    17341 gctggatttc ccccggtatc ttcggcgcat ttgccaggga attactctcg ccgattcgca
    17401 gacatcgacg gagctgcaaa tcgcggcggc gttttgcctg cccgcccaca ctggagga
//
LOCUS       DASQJS010000174         7419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1666641, whole genome shotgun sequence.
ACCESSION   DASQJS010000174 DASQJS010000000
VERSION     DASQJS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666641"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VEW08_07375"
     CDS             <1..1158
                     /locus_tag="VEW08_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HYJ40592.1"
                     /translation="RIGPTAMGPAEKFFNSYPLVHTGGVYEGSRAVSPDRRVFILTRS
                     GFAGLQRHAAAVWSGDIASRWDDLYNQISAGVSVGYSGLPNWTFDIGGFANEGRYSTA
                     KPAAADLEEWRELNLRWFQFGAFAPLFRSHGEFPFREIFNLAPQGSEVFASLVWHDKL
                     RYRLLPYTYTLAADTFHRDGTIMRGLAMDFADDVAARDVRDEFLFGKAFLVAPVHEHK
                     ARTRQVYLPAGADWYDFHSGTKTAGGQSVEAAAPLARMPLYVRAGSIVPVGPEVQYTS
                     EKPGAPITLFVFTGTDGSFDYYEDDGVSYGYERGEFARIPLRYDAAKGALTIGARSGS
                     YAGMPEERTFKVRWIRAGGKAPADLDAAADATVEYKGAELTVPGGESGENK"
     gene            1275..2738
                     /gene="zwf"
                     /locus_tag="VEW08_07380"
     CDS             1275..2738
                     /gene="zwf"
                     /locus_tag="VEW08_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HYJ40593.1"
                     /translation="MAKVPAGVGPRFPQVVVLVGATGDLARRKLIPGLMHLARVGFIP
                     GCRIIGVSLDQLDTDSFRNFARESLTEFSARRVSDDDWSSFASSIDYVSLSAGPAALA
                     EAVTRAEASIGGESRRLHYLSVPPSAALSAVRMLGDAGLVQNSRIIMEKPFGTDLASA
                     VQLNAKLHEVFAEEQIFRIDHFLGKEPAQNILAFRFGNGLFEPIWNRNFIDHVQIDVP
                     ETLGLGKRSGFYESTGAYRDMVVTHLFQILGFMAMEPPTHLAPKPISEEKNKVFRSML
                     PIQPADVVRGQYIGYRDEEGVAPESDTETFIALKCSIDNWRWAGVPFYLRTGKRLAEG
                     QRIISIAFREPPKSMFPEGSGVGAHGPDHLTFDLADAAKTSLSFYGKRPGPGMRLDKH
                     SLQFAMNDTGQIGDVLEAYERLILDAMRGDHTLFTTAEGIERLWEVSVPLLESPPPVR
                     TYSPGSWGPNAIHQLVAPHAWRLPFERGWRDPNKQGS"
     gene            2958..5225
                     /locus_tag="VEW08_07385"
     CDS             2958..5225
                     /locus_tag="VEW08_07385"
                     /inference="COORDINATES: protein motif:HMM:NF007349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HYJ40594.1"
                     /translation="MSHLSIAVAVSLALSAAAAKAQTANEEAKVMPKVSVAAPEPEET
                     VKVDRVSSPKFTQELLDTPQTIAVVSKEVLKQQGATTLSQALRNTPGVTFLLGENGNT
                     ATGDSIFMRGFDTQGSIFIDGIRDLGTVTRDTFNTEQVEIAKGPAGPDYGRGAASGYV
                     NMASKVPTVDSYMGGTASYGTANNGRITGDINHEFGDSGTAVRLNVMGQDGEVDGRDF
                     IERKGWAVAPAIAFGLDGDTRSYFYLLHTEQNNIPDGGVTTLGLDGFYNRDLDPNPPA
                     TTPPLPPPRAPGVIPAPVDTENYYGYRSDFEETSGTMFTARFEHDFSENVSLRNSTRY
                     GRLEQFYILTGVNLVIIPSPDPDTWTVNRTRQSKFQENTLMTNQTNLTINVATGSVSH
                     AITTGVEFIDEEQYNPAYAPASLGTLVPANPYDPGLTDAPPNYAPARNGAYTRGETQT
                     IGVYAFDTLSFAEKWEVTGGFRVDSYDTDFEGVTASVATPPVLTTSRLGTDDSLFSYK
                     VGVLYKPQPNGSIYLSHAISKQPPGGANFTLTPPNGANNANRTDLEPTEGENLELGTK
                     WEFRDGALAVTAALFDSSNKNELVPDATNPTVFVQVGEREVKGIELGIVGKVTENWDL
                     TAGIAKMDTEVVKGSTTQQGSQINWSPELTFSSWTTYRTPFGLSIGGGARYIDTVARS
                     ISNNVLPTANVPTAPEYWVVDAMLGYQVNDKVNIQLNGYNLLDEEYIATLNNGGSRFI
                     PGAPLSALLTVNFTF"
     gene            5251..5931
                     /locus_tag="VEW08_07390"
     CDS             5251..5931
                     /locus_tag="VEW08_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004757131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HYJ40595.1"
                     /translation="MMIHVPKVLTPEQVAECRRLLDTAEWTDGKATVGEQGALVKKNR
                     QLPELSPVGRQLGEMILTALARNPLFFSAALPLKTVPPLFNRYEGGEQYGTHIDGAVR
                     AVPGSSHFIRTDLSSTLFLTDPSAYDGGELVVFDTFGQHEVKLPAGDLVLYTSGSLHR
                     VNPVTRGARVSSFFWTQSMVADERHRETLYRLDQTIQKVRGKHGETDETVALAGHYHN
                     LLRMWSEI"
     gene            complement(5928..7076)
                     /locus_tag="VEW08_07395"
     CDS             complement(5928..7076)
                     /locus_tag="VEW08_07395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYJ40596.1"
                     /translation="MKTNSNPATIILLVAAGTFLGSCGGGDNGIQSWAPPPDANPGLP
                     ETVSVAPADTGDGWSVSSPTAESMDSPAVLGTLQSIRDGRFSGVDALVVVRHGRLAAE
                     GYFNGYGPETLHDLRSTGKSFTSALAGIAIEQRLFALDDPIATLIPQFENHANMDARK
                     RAITIRHLLDMRSGLECNDWSSASRGNEERMYNTRDWVGFILDLPMVNDPGAVASYCT
                     GGVVVLGQVIAWRSGMALDAYAAAYLFAPLGIQQSTWRRSPDGSATGGGGLRLRPRDA
                     ARFGQLYLNGGVWNGARVVPADWVTRSRERVTTLGNDGYGLLWWKRSFAHRGVMVESF
                     FTSGNGGNFIFVFPALDLIAVFTGSNYNSPMGDQPFDIVGNYILPAVR"
     gene            7172..>7419
                     /locus_tag="VEW08_07400"
     CDS             7172..>7419
                     /locus_tag="VEW08_07400"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HYJ40597.1"
                     /translation="MNEIDQELVGRLLRGDRAAFDEFFNEYYPKLYRFVKRRMPFDAA
                     ACEDVAQATLCRALESLHGYRGEAALMTWLCTLCRRELS"
BASE COUNT         1398 a         2411 c         2332 g         1278 t
ORIGIN      
        1 cgcatcggcc ccacggcaat gggaccggcc gagaagttct tcaactccta tccactggtg
       61 cataccggcg gcgtttatga aggttcacgc gcggtgagcc ccgacaggcg cgtgttcatt
      121 ctcacgcgct ccggattcgc cggcctgcag cgtcatgccg ccgcggtgtg gagcggtgac
      181 atcgcatccc gatgggatga tctctacaac cagatttccg cgggcgtgag tgtgggctat
      241 tcggggctgc ccaactggac gttcgatatt ggcggattcg ccaatgaagg gcgctacagc
      301 acggcaaaac ccgcggccgc ggatctcgag gaatggcgag agctcaactt gcgctggttc
      361 cagttcggcg cgttcgcacc gctattccgt tcgcacggtg agttcccgtt ccgtgagatc
      421 ttcaatctcg cgcctcaagg atcggaagtc ttcgccagcc tggtgtggca cgacaagctg
      481 cgttatcgac tgctgccgta cacctacacg ctggctgcgg ataccttcca ccgcgatggc
      541 accatcatgc gcggcctcgc catggacttc gccgatgacg tcgccgcgcg cgacgtgcgc
      601 gatgagttcc tgttcggcaa ggccttcctg gtcgctccgg tgcatgagca caaggcccgc
      661 acccgccagg tctatctacc ggccggggcc gactggtatg acttccattc gggcacgaag
      721 accgcgggcg ggcagagcgt ggaggcggcc gcgcccctgg ctcgcatgcc actttatgtg
      781 cgtgcaggat ccatcgtccc ggtggggccg gaggtgcagt acacgtccga gaagcccggc
      841 gcgccgatca cgctgttcgt gttcaccggt accgacggca gcttcgacta ctacgaagat
      901 gacggcgtga gctatggtta tgaacgcggc gaattcgcgc ggattccgct gcgctacgac
      961 gccgccaagg gcgcgctgac catcggcgcg cgatcgggca gctatgccgg catgccggag
     1021 gagcgcacgt tcaaggtgcg ctggatcagg gcgggcggca aggcgccggc ggatctcgac
     1081 gcggcggcgg atgccaccgt ggagtacaag ggcgcggaac tcacggtgcc gggtggggaa
     1141 agtggggaga acaagtgacg ggctgagata tgtggggcgt acagttgctc tccccacttt
     1201 ccccacccgg caccgaccgt ggtaaaacct tcgtcacgag gggtgttctc tactaataaa
     1261 aatgggagtg caccgtggcg aaggttccag caggggtcgg accgcgtttt ccgcaagtgg
     1321 tcgtgctcgt cggcgcgacc ggcgatctcg cgcgccgcaa gctcatcccg gggctcatgc
     1381 acctggcgcg cgtcggtttc attcccggct gccgcatcat cggtgtgtcg ctcgaccagc
     1441 tcgacaccga cagcttccgt aatttcgcgc gcgagtcgct gactgaattt tcggcgcgca
     1501 gagtttccga cgacgactgg agcagcttcg cgagctcgat cgactacgtg tcgttgtccg
     1561 ccggcccagc cgcgctggcc gaggcggtta cgcgcgccga agcgtccatc ggtggggaga
     1621 gccgccgcct gcattacctg tccgttccgc cgagcgcggc gctctccgcc gtgcgcatgt
     1681 tgggcgacgc gggcctggtg cagaactcgc gcatcatcat ggaaaagccg ttcggtaccg
     1741 accttgcgag cgcagtgcag ctcaacgcca aactccacga ggtgttcgcc gaagagcaga
     1801 tattccgcat cgaccatttc ctcggcaagg aacccgcgca gaatattctc gcgttccgtt
     1861 tcggcaacgg cttgttcgag ccgatctgga atcgcaactt catcgaccac gtgcagatcg
     1921 acgtgccgga aacactcggg ctcggcaaac gctcgggatt ctatgaatcc acgggcgcgt
     1981 accgcgacat ggtggtgacg cacctgttcc agatcctggg gttcatggcg atggagccgc
     2041 ccacacacct ggcgccgaag cccatcagcg aagagaagaa caaggtgttt cgcagcatgc
     2101 tgccgatcca gcccgccgac gtggtgcgcg ggcagtacat cggctatcgc gacgaggagg
     2161 gcgtcgcgcc ggaatccgac accgagacct tcatcgcgct gaaatgctcg atcgacaact
     2221 ggcgctgggc cggcgtgccg ttctatctac gcaccggcaa acggctggcg gaaggccaac
     2281 gcatcatctc catcgcattt cgcgagccgc cgaagagcat gtttcccgaa ggctcaggcg
     2341 tcggcgcgca tggccccgat cacctgacct tcgatctggc cgacgccgcg aagacctcgc
     2401 tctcgttcta cggcaagcgc ccggggcccg gcatgcgcct cgacaagcac agcctgcagt
     2461 ttgcgatgaa cgacacgggg caaatcggcg acgtcctgga ggcctacgaa agactgatcc
     2521 tcgatgccat gcgcggcgat cacaccctct tcaccacggc cgagggcatc gagcggcttt
     2581 gggaagtgtc cgtcccgctg ctcgagtcgc cgccgccggt gcgcacttat tcgccgggtt
     2641 cgtgggggcc gaacgccatc caccagctcg tcgccccgca cgcgtggcgg ctgccgttcg
     2701 aacgcggctg gcgcgatccg aacaagcagg ggagctagcg cggacataat ccgcccgggc
     2761 cggagccatt aataagagtc ggcccggatg acaatcattc ttgattgcaa tcaagaatga
     2821 ttcgcaataa gatctggccg ctgctggaat ggagtgatcg ccgtttcagc cgctgccccg
     2881 gcagcaatgc cagccactcc cgaccgggat agccagagag cgcggggacc gcatcaaccg
     2941 catttgggag ctaaaccgtg tcgcaccttt ccatcgctgt ggccgtgagt cttgcgctgt
     3001 ccgcagccgc cgccaaagca caaaccgcca acgaagaagc gaaggtcatg ccgaaggtgt
     3061 cggtggcggc gcccgagccc gaagagacgg tgaaggttga ccgcgtgtcg tcaccaaaat
     3121 tcacacagga actcctcgac acgccacaga ccatagccgt cgtgagcaag gaagtgctca
     3181 agcagcaggg cgccaccacg ctctcacagg cgctgcgtaa cacacccggc gtcacgttcc
     3241 tgctgggcga gaacggcaac accgcaaccg gcgattccat cttcatgcgt ggcttcgaca
     3301 cgcagggttc catcttcatc gacggcatcc gcgatctcgg caccgtcacg cgcgacacct
     3361 tcaacaccga acaggttgaa atcgccaagg gtccggcggg tccggactat gggcgcggcg
     3421 ccgcgtcggg ctacgtgaac atggccagca aggtaccgac cgtggatagt tacatgggcg
     3481 gcacggccag ctacggcacc gccaacaacg gccgcatcac cggtgacatc aaccatgagt
     3541 tcggggacag cggcaccgcc gtgcgcctca atgtcatggg ccaggatggc gaggtcgacg
     3601 gccgcgactt catcgagcgc aagggctggg ccgtggcgcc ggcgatcgcg ttcgggctcg
     3661 atggcgacac gcgcagttac ttctatctac tgcacacgga gcagaacaac attcccgacg
     3721 gtggcgtcac cacgctcggg ctggatggct tctacaaccg cgatctcgat ccgaatccgc
     3781 ccgcgaccac gccgccgctg ccgccgccgc gcgcaccggg cgtgatcccc gcgcctgtcg
     3841 acaccgagaa ttactacggt tatcgaagcg atttcgaaga gacctcgggc acgatgttca
     3901 cggctcgttt cgagcatgat ttcagcgaga acgtgtcgct gcgcaacagc acgcgctacg
     3961 gccgtctcga gcagttctac atcctcacgg gtgtgaacct cgtgatcatc ccgagtccgg
     4021 acccggatac gtggacggtc aaccgcacgc gtcagagcaa gttccaggag aacacgctca
     4081 tgacgaacca gaccaacctc acgatcaacg tcgcaacggg ttccgtgagc cacgccatca
     4141 cgacgggcgt cgagttcatc gatgaggagc agtacaaccc cgcctatgct cccgcgagcc
     4201 tcggtacgct cgtgccggcg aacccgtacg acccgggtct caccgacgcc ccgccgaact
     4261 atgcgccggc gcgcaacggc gcgtacacgc gcggcgagac ccagaccatc ggcgtctatg
     4321 cgttcgatac cctgagcttc gccgagaagt gggaagtgac gggcggattc cgcgtcgact
     4381 cctacgacac ggacttcgag ggcgtcacgg cgagcgtcgc gacgccgccg gttctgacca
     4441 cctcgcggct cggaaccgac gactccctgt tctcgtacaa ggtgggcgtg ctgtacaagc
     4501 cgcagccgaa cggcagcatc tatctgtcgc atgccatctc gaagcagccc ccgggcggcg
     4561 ccaacttcac tttgactccc cccaacggcg ctaacaacgc gaaccgcacc gatctcgagc
     4621 cgaccgaggg cgagaacctg gagctcggca cgaaatggga gttccgcgat ggcgcgctgg
     4681 ccgtcaccgc cgcgctcttc gacagcagca acaagaacga actggtgccg gacgccacca
     4741 atcccaccgt gttcgtgcag gtgggcgagc gtgaagtgaa gggcatcgag ctgggtatcg
     4801 tgggcaaggt gaccgaaaac tgggatctga ccgcaggcat cgccaagatg gataccgaag
     4861 tcgtgaaggg cagcaccacg cagcagggct cgcagatcaa ctggtcgccc gagctcacgt
     4921 tctcgagctg gaccacctac cgcacgccgt tcggcctctc catcggcggc ggcgcgcgct
     4981 acatcgacac tgtggcgcgg tcgatctcca acaacgtgct gcccaccgcg aatgtgccaa
     5041 ccgcccccga gtactgggtg gtggatgcca tgctgggata ccaggtgaac gacaaggtga
     5101 acatccagtt gaacggttac aacctcctcg acgaggagta catcgccacg ctcaacaacg
     5161 gtggctcgcg attcatcccg ggtgcgccgc tgtcggcgct gctaaccgtg aacttcacgt
     5221 tctaacagcc actgtgcgag cattcggccc atgatgatcc atgtgccgaa ggtcctgact
     5281 cccgagcagg tggcggaatg ccgtcgcctg ctcgataccg ccgaatggac tgacggcaag
     5341 gcgaccgtcg gagagcaggg ggcgctggtt aaaaagaacc gccagttacc cgagctgtcg
     5401 ccggtcgggc gccagttggg cgagatgatc ctcaccgcgc tcgcgcgcaa tccgctgttt
     5461 ttctcggcag cgctgccgct caagaccgtg ccgccgttgt tcaatcgcta cgagggcggc
     5521 gagcagtacg gcacccatat agacggcgcg gtgcgcgcgg tgcccggcag ctcgcacttc
     5581 attcgcacgg acctttcgtc cacgctgttc ctcaccgatc cgtccgcgta tgacggtggt
     5641 gagctcgtgg tgttcgacac ctttggccag cacgaagtca aactaccggc gggcgacctc
     5701 gtgctgtaca cgtcgggaag cctgcaccgc gtgaatcccg tgacgcgcgg cgcacgcgtc
     5761 agctcgttct tctggaccca gagcatggtg gcggacgaac gccaccgcga aactctctac
     5821 cggctcgacc agaccatcca gaaggtccgc ggcaaacacg gcgagacgga cgagaccgtg
     5881 gctctggccg ggcactatca caacctgctt aggatgtggt cggagatcta gcgaaccgcg
     5941 ggcagaatat agtttccgac gatgtcgaag ggctgatcgc ccatcggcga gttgtagttg
     6001 gagccggtga acaccgcgat caggtcgagc gccggaaaga cgaagatgaa attgccgccg
     6061 ttgcccgacg tgaaaaagct ctccaccatg acgccgcggt gcgcgaagga gcgtttccac
     6121 cacaacaacc cgtatccgtc gtttcccagc gtggtgactc gctcgcgcga tctcgtcacc
     6181 cagtcggctg gaaccacgcg ggcgccattc catacgccgc cgttgaggta gagctgtccg
     6241 aacctcgcgg cgtctcgtgg ccggagcctc aggcctccac cgcccgttgc gctgccatct
     6301 ggcgatctcc gccatgtgga ctgctgtatg ccgagaggcg caaatagata ggcggcggcg
     6361 tatgcgtcca gcgccatgcc cgagcgccac gcgatcacct gccccaggac gacgactccg
     6421 ccagtgcagt acgacgcgac cgcgccggga tcgttcacca tgggcaggtc gaggatgaag
     6481 ccgacccagt cgcgcgtgtt gtacatgcgc tcctcgttgc ctcgcgaagc gctgctccag
     6541 tcgttgcact cgaggccgga gcgcatgtcc agcaggtgcc ggatggtgat cgcgcgcttg
     6601 cgcgcgtcca tgtttgcgtg attctcgaac tgcgggatca acgtcgcgat gggatcgtcg
     6661 agcgcaaaca accgctgttc aatggctatg ccggcgagcg cggaagtaaa gctcttgcct
     6721 gtcgagcgca ggtcgtgaag cgtttcgggt ccatatccgt tgaaatagcc ttctgccgcg
     6781 agccgcccgt gtcgcaccac caccagcgcg tcgactccgg aaaagcgtcc gtcacggatt
     6841 gactggaggg tgccgagcac ggcgggggag tccatgcttt cagcggtcgg ggaggaaacg
     6901 gaccaaccat cgccggtatc tgcgggtgcg acgctcactg tctcgggcag tcccggattg
     6961 gcgtccggcg gcggagccca ggattggatc ccgttgtccc cgccgccgca ggaacccaga
     7021 aaggtgcccg ccgcgaccaa caggatgatg gttgccgggt tcgagttggt tttcatatga
     7081 gctgagtggt gtttgcgggc cacaatcctt cgtctcactt tcgcgcaatg gggcgcgaag
     7141 gatttttcgc cgccgggcac actaaccccc gatgaacgaa atcgaccagg agctggtcgg
     7201 acgcctgttg cgcggggacc gtgcggcgtt cgatgagttc ttcaatgagt actacccgaa
     7261 gctgtatcgc ttcgtgaagc gccgcatgcc gttcgacgcg gcagcctgcg aagacgtggc
     7321 ccaggccacg ctgtgccgcg cgctcgagag cctccatggc tatcgcggcg aggcggcgct
     7381 catgacctgg ctttgcaccc tctgccggcg cgagctctc
//
LOCUS       DASQJS010000175         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2925145, whole genome shotgun sequence.
ACCESSION   DASQJS010000175 DASQJS010000000
VERSION     DASQJS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2925145"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /locus_tag="VEW08_07405"
     CDS             <1..1541
                     /locus_tag="VEW08_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HYJ40598.1"
                     /translation="RMYLVNWIGERVVADLRSAVYARVIRMDPAFFEVTKTGEVLSRL
                     TTDTTLIQSLSGIGLSILLRSTVSFLGSLVLLLLTSLKLALIIFALIPTVLAPMLLFG
                     RRVRRLSRDSQDRVADTSGLAGETLNAIQTVQAFTLEGLQGSRYDAAVEESFSTAVRR
                     TRVRAWLIAFAIMTMFSAVTLVLWLGARSVLDGTMSGGDLAKFLSYALFMGTAVASLS
                     EMWGELQRASGAMERTIELLQSRPSIVTPTAPIALEVTRGAIAFEHLSFNYPSRPDTR
                     ALDDFHLEIAPGETVAFVGPSGAGKSTVFQLLLRFYDPASGRILIDGLDIARADPSVV
                     RAHIGLVPQETMIFGASARENIRYGRPGASDTDIEAAARAAAADEFLERLPEGYDTFL
                     GERGARLSGGQKQRIAIARAILKNPPILLLDEATSSLDSESEQLVRDALEKLKRNRTT
                     LVIAHRLATAINSDRIVVVDRGRIAGIGRHEELLRDNALYARLAALQFGERGDSPWPI
                     SMDS"
     gene            1517..1876
                     /locus_tag="VEW08_07410"
     CDS             1517..1876
                     /locus_tag="VEW08_07410"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HYJ40599.1"
                     /translation="MAYIDGFLIPVPKRNAAKYKKMSTQAGKVWMDHGALEYHECVAD
                     DVMYGKYTSFPQGVKLKKGEQVWFSWIVYKSRKDRDRVNAKAMKDPRLAHMMDPKAMP
                     FDGKRMVFGGFKMVVDL"
     gene            1919..2506
                     /locus_tag="VEW08_07415"
     CDS             1919..2506
                     /locus_tag="VEW08_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HYJ40600.1"
                     /translation="MSKVLVVDGDRAARDALVTCLKFVGMEAHGAENAAAARCWLETD
                     VADVLVLADEIADGVSTDPGRSIIVLTRPTISPGRVVERVEWLIEERRLHQDVILTFG
                     GLSLDVSAGRVSSAGTEVALGHIETRLLAFFMGIPDKVMSRAQLSRRMWPANVRVAER
                     TVDMHIRRLRLALAQLDCAGYIQTVRCSGYRFSAF"
     gene            2572..3372
                     /locus_tag="VEW08_07420"
     CDS             2572..3372
                     /locus_tag="VEW08_07420"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HYJ40601.1"
                     /translation="MHDPDRIHKVRFRSVFVSDVHLGSRDCRAGELLKFLESIEADYL
                     FLVGDIVDFWSLRRSFYWPEAHNEVLRAILAMAKEGTRVIYVPGNHDENIREFCGSLF
                     GNVSIRRRYVHATADGREFLVMHGDEFDSAVKCSRWLARFGATAYEFMMRINRGVNLC
                     RRAVGLPHWSLASYLKLRLANAVRYVEAFEHAAAQAALSRNLAGIVCGHIHRPGLRQI
                     DGVLYCNDGDWVESCTALVETSSGELSLWYAEKMYPHQQQEDLVGIAA"
BASE COUNT          631 a         1086 c         1130 g          687 t
ORIGIN      
        1 tgcgcatgta cctcgtgaac tggataggcg agcgcgtggt ggcggacctg cgcagcgcgg
       61 tgtacgcgcg ggtcattcgc atggacccgg cgtttttcga ggtgaccaag accggcgagg
      121 tgctctccag gctaactacc gacaccacgc tgatccagtc gctgtccggc atcggtttgt
      181 cgatcctgct gcgctcgacg gtgagcttcc tggggtcgct ggtcctgctg ctgctgacca
      241 gcctgaaact cgcgctcatc atcttcgcgc tgatcccgac cgtgctcgcg cccatgctgt
      301 tgttcggccg gcgcgttcgc cgcctgtcac gcgattcaca ggatcgcgtg gccgacacct
      361 ccggtctcgc cggcgaaacc ctgaatgcca tacagacggt gcaggcgttc acgctcgaag
      421 ggctgcaggg cagccgttat gacgcggcgg tcgaggagag tttcagcacg gccgtacgcc
      481 ggacgcgggt ccgggcctgg ctgatcgcgt ttgccatcat gaccatgttc tcggcggtca
      541 cgctggtgct gtggctgggc gcacgctcgg tgctcgacgg caccatgtcc ggcggcgacc
      601 tggccaaatt cctgagctac gcgctcttca tgggcacggc ggtcgcgtca ctctccgaga
      661 tgtggggcga gttgcagcgg gcatcgggcg ccatggagcg caccatcgag ctgttgcaat
      721 cgcggccgtc catcgtgacc ccgactgcgc ccatcgctct cgaagtcacg cgcggcgcta
      781 tcgccttcga gcacctgtca ttcaactacc cgtcgcggcc cgacacgcgc gccctcgatg
      841 atttccacct cgagatcgcg cccggcgaaa ccgtggcttt cgtcggtccc tcgggtgctg
      901 gcaagagcac ggtgttccag ctgctgctgc gattctatga cccggcgtcg ggacgaatcc
      961 tcatcgacgg gctggacatc gccagggcgg atccatccgt cgttcgggcg cacatcggcc
     1021 tcgtgccgca ggaaaccatg attttcggcg cgagcgcgcg cgagaacatt cgttatggcc
     1081 gccccggcgc cagcgacacc gacatcgagg ccgcggcgcg cgcagcggcc gcggacgaat
     1141 tcctcgaaag actgccggag ggttacgaca ccttcctcgg cgaacgcggc gcgcggctgt
     1201 cgggtggtca aaagcagcga atcgccatcg cgcgggcgat cctcaagaac ccgcccatcc
     1261 tgctgctcga cgaggccacc agctcactgg attccgagag cgagcagctg gtgcgcgacg
     1321 cgctcgagaa gctcaagcgc aaccgcacga cgctggtcat tgcgcaccgg ctggccacgg
     1381 ccatcaattc ggaccggatc gtggtcgtcg accgcggacg catcgccggc attggccggc
     1441 acgaagaact gctgcgggac aacgcgttgt atgcgcggct cgcggcgcta caattcggcg
     1501 aaagaggaga cagcccatgg cctatatcga tggattcctg atcccggtgc cgaagcgaaa
     1561 cgcggcgaaa tacaaaaaga tgtctaccca ggcgggcaag gtgtggatgg accacggcgc
     1621 gctcgaatat cacgagtgcg tggccgatga cgtcatgtac ggcaagtaca cctcgttccc
     1681 ccagggcgtg aagctcaaga agggcgagca ggtttggttc tcatggatcg tctacaagtc
     1741 gcgcaaggat cgcgatcgcg tgaacgcgaa ggcgatgaag gatccgcggc tcgcgcacat
     1801 gatggacccg aaggccatgc cattcgacgg caagcgcatg gtgttcggcg gcttcaagat
     1861 ggtggtcgat ctctagacgc tccatcaatc tgcaacgtca tccctgtcta atcgccggat
     1921 gtcaaaagtc ctggtggttg acggcgatcg tgcggcgcgc gatgcgttgg ttacctgtct
     1981 gaagttcgtc ggcatggagg cgcacggcgc ggaaaatgcg gccgcggcac ggtgttggct
     2041 cgagactgat gtcgccgacg tgctggtcct ggccgatgaa atcgcggatg gagtgtccac
     2101 cgatccgggc cgatcgatca tcgtgttgac gcggccgacg atatcgcccg gtcgcgtggt
     2161 ggagcgagtc gagtggctga tcgaagaacg caggctgcac caggacgtca tcctgacgtt
     2221 tggtgggcta tcgctcgacg tttcggctgg tcgtgtctca tccgcaggga ctgaagtcgc
     2281 gctgggccac atcgaaactc ggctgctggc ctttttcatg ggtattcccg acaaagtcat
     2341 gtcgcgtgcc caattgtcgc ggcgaatgtg gcccgcaaat gtgcgtgtcg ccgagcgtac
     2401 ggtggacatg catatccgcc gtctgcgcct ggcgctcgcg cagctcgatt gcgccggcta
     2461 catacaaacg gtgcgctgct ccgggtaccg cttctcagcc ttttagcgaa gacagtcgtg
     2521 taagtcgcgg agtcataaag atgaaatgga ccgcgagcac actttgtttc catgcacgat
     2581 ccggatcgca tccacaaagt ccgcttccgt tcggtgttcg tatccgatgt ccatctcggt
     2641 tcgcgggatt gccgggccgg cgagctgctg aaattcctgg aatcgatcga agccgactat
     2701 ctgtttctcg tcggcgacat cgtggacttc tggagtcttc gccggtcctt ctactggccc
     2761 gaagcgcaca acgaggtgtt gcgcgcgatt ctcgccatgg ccaaggaagg cacgcgggtc
     2821 atctacgtgc ccggaaacca cgacgaaaac atccgtgagt tctgcggctc gctgtttggc
     2881 aatgtgagca ttcgccgccg ctatgtgcat gccacggccg acggcaggga attcctggtc
     2941 atgcatgggg acgagttcga ctcggcggtg aaatgcagcc gctggctcgc gcgttttgga
     3001 gctaccgcgt acgaattcat gatgcgcatc aatcgtggtg tgaatctgtg ccggcgcgcc
     3061 gtcggactgc cgcactggtc gctcgccagc tacctcaaac tacgactcgc gaacgccgtg
     3121 cgctacgtcg aagcattcga acatgccgcg gcgcaagccg cgctcagccg taatctcgcg
     3181 ggcatcgtct gcggccacat ccaccgcccg gggcttcggc agatcgacgg tgtgctctat
     3241 tgcaacgatg gcgactgggt cgaaagctgc acggcgctcg tggagaccag cagcggcgaa
     3301 ctgtcgctgt ggtatgcgga aaagatgtac ccgcatcagc agcaggagga tctcgtcggg
     3361 atcgccgcgt aagccgacag tacagttgtc aggattacga gggtttctct cacgctactt
     3421 ggccgaaggg ttgtgaaata atcctgcgcc atggagcttc gctttcgcat tctcaatgtc
     3481 ttcacgtcag gcagccgcct ctccggaaac ccgctgtgcg tggtcgaaga cggc
//
LOCUS       DASQJS010000176         9629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1667306, whole genome shotgun sequence.
ACCESSION   DASQJS010000176 DASQJS010000000
VERSION     DASQJS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667306"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2076
                     /locus_tag="VEW08_07425"
     CDS             <1..2076
                     /locus_tag="VEW08_07425"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40602.1"
                     /translation="NADGTGIHQISFNPSSDLYPQVLQNGRVLFTRWDRAPGRDGLHL
                     YTSNPDGTDMQLHYGAVSHLTGTVDPTTGQPTSIEFMRAREMSDGRLMVLTRQRTDVD
                     FGGNLTIIDTNRFVENTQPLLTNAGMPGPAQTPATLNPVSTLPGPSEGGRFQSGFPLW
                     DGSGRILASWSQCRLLDTVDPTRIVACTDERLANPNAQAAPPLYSIWMLNPAQNTLLP
                     VMQPVEGIMVTEAVAAQPRTPLPSVIVDQQPPALDADLVAEGAGLLSIRSVYDIMGVD
                     QARTTAGTATSIANVSNPSLAQYGSRLARFVRIEKPVSIPDDDDIADPDNSAFGPTGV
                     MREIVAYAPVEPDGSVRMKVPANVAFQISVLDQNGRRFSPIHRSWLQVRPGEVIECNG
                     CHVRNAVTPTTSPLVHGRKGLFNAVNTGAPAGPFPGAVTSISSDGGDTMARARSRAAS
                     SCVDPITMVENCGLQSITPSANVVYNEIWKPAALPTDSFSYSYQQLTTASPANVGCFP
                     VWTPTCRITLHYATIGTRAGQIHPLWSVPRPAAGVDTNNDGILEFPQTCTNCHSRVDP
                     VDAAVVQLPAASLELTDEVSDEDALQLRAYRHLLFNRPQLELVMGAIQQVTIPGPPDD
                     NGIPSQIPQTFPPSMGASNARGSRFFTVMNNTTHAGMLSTAELRLLSEWLDIGAQYYN
                     DPFPPTPQN"
     gene            2189..2848
                     /locus_tag="VEW08_07430"
     CDS             2189..2848
                     /locus_tag="VEW08_07430"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HYJ40603.1"
                     /translation="MFVSEPYLELAIGPGRGYAVFHVVEREQSVDVLFRRTDWFKVRT
                     EQGVEGWARARDMRRTKLADGSPFVFNLGDRAGFTTHDWEIGIGGGDYGGANLIAAYG
                     SYSLTDNMKIDLSLSQFLGNSSNGWKAEIGLQHVLFPEWRFSPFLTLGTGYVETSPRA
                     TIVLPLDRADQTAYAGAGARFYLTRRFFLRADYRWHTVFTSRDDNEELEEWKVVIACF
                     F"
     gene            2818..3540
                     /locus_tag="VEW08_07435"
     CDS             2818..3540
                     /locus_tag="VEW08_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HYJ40604.1"
                     /translation="MESRNRLLLLTAVCCATLMLSGCASFRWPWGKEKVEEQQTDTEA
                     AEEEAEEQDVSPPRVIEPNVTRRKIKTPRIDNENWEVGAGLGFLSIEDFGTSSTYAAQ
                     LSYHVTEDFFFRLDAGQSTAGETSFETLGGDIELLTGDERRFTYYSLSLGYNFLPGEV
                     YLGRNLAMNSSFYLLGGIGSVDFAGDDRLTINLGAGYRVLPTDWLAISIGVQDRVFDS
                     DLLGETKVTNNIEMLLSATVFF"
     gene            3554..4078
                     /locus_tag="VEW08_07440"
     CDS             3554..4078
                     /locus_tag="VEW08_07440"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYJ40605.1"
                     /translation="MMARTLKAKGIGRVAKGLALALVFVLPAFAASSSGPAPAFQLAG
                     RNGKAIDLSQFKGQVVMINFWATWCKPCRDEMPLLEDIYKKYKPMGFTLVGVNVEPDA
                     KDAEAWLGKLAKPVSFPIAFDTDSKVSKMYKVAVMPSTVFVDRKGNIRIMHKGYKAGD
                     ENTYLSQIRSMLKE"
     gene            4103..4330
                     /locus_tag="VEW08_07445"
     CDS             4103..4330
                     /locus_tag="VEW08_07445"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HYJ40606.1"
                     /translation="MRTRGWLVLSALAVLSAGCSSIEPWVKPYDRENLADPIMAFTRA
                     PISQDFLDHVFESREGSRGATGGGGGGCGCN"
     gene            4346..5536
                     /locus_tag="VEW08_07450"
     CDS             4346..5536
                     /locus_tag="VEW08_07450"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HYJ40607.1"
                     /translation="MKAHYTRLVLFVITCLAGTATAGVLPEDRADLLYFRYDGGGVEI
                     TGPSVLVRKSIGEHFSVAANYYIDMVSSASVDVETSASPYEDERTQSSLALDFLHGKS
                     TYSVGVVKSDESDYVADTWYANVSHDMFGDLTTISFGFKNGQNEVFRNVKIDGVKVND
                     PNFADEMESKSFNVSVSQIITKNLVMIGQYEVITDEGFLRSPYRSVRFFIDPFTQGQQ
                     SEIYPNTRSSNAASVRAKYFLPYRAAVDAMYRFYTDTWGVLGHTGELGYVHPLPNHWI
                     FEGRVRYYAQTSPADFYQDIFPRADYANFMARDKELATYDAITAGVSATYEFKIDRFP
                     WLAKGALNLRYDYMTVNYDDFRDARYSLGSFGTLPDEPLQPGTEPLYKLNANIIQFYI
                     SAFF"
     gene            6126..6752
                     /locus_tag="VEW08_07455"
     CDS             6126..6752
                     /locus_tag="VEW08_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40608.1"
                     /translation="MSSLQLPRSSPARIEDFEREQLPEVVGQDPEAAARFAEMQTESE
                     LRKLAIRQVAQLWGALDANRAVNWARTLPDAAERDAAFIDIANGLSSVDPGRGVELRE
                     QFVAATRPDSALNNLVQQWAGYDFDAALAWTNARAPGAQRDDLMQRLIYVRAAAGKPA
                     DAARLVTESSLSGAARTAAIASVAREWASRDGDAAQEWIQTLEPATLN"
     gene            complement(6755..8191)
                     /locus_tag="VEW08_07460"
     CDS             complement(6755..8191)
                     /locus_tag="VEW08_07460"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HYJ40609.1"
                     /translation="MPHTSTPQLRAANYLLFALTAAVCCSHPALAQSTPTQIHTPPQQ
                     DPGVYSGRLKVDYPTPYEPATVEQIRATLERVHAYVDQAAPVQVIDGETGAPVTDLTN
                     LPTRVALARTDMQILTYEWGVTYAGMLRSAQVTGDKRYSDYTHQRVLAIATLAAHAKK
                     NLPPGTTHANYPVSQHGLGLRSILLPGRLDDSGAMCAALIKAELAGLGDRKLRPWIDN
                     YVQYVSSGQFRLKDGTLARNRPLPNSLWLDDLYMSVPCLAQAGKLTGERRYYDDAAKQ
                     ILQFSDRMFVKDKGLFMHAWVQDMKHHPAFHWARANGWAIMATVELLEVLPEDHQARP
                     RILALLRAHAAGLTAVQGHAGRWHQLLDRSESYEETSASAMFVFAIARGINRGWLDPL
                     AFGPAVSLGWNAVVTRVNAKGQVEGTCVGTGVGWDPMFYMYRPVHVLAAHGYGPVLLA
                     GAEMIELRKVYPSITHDGGVQFGKAKEN"
     gene            complement(8300..9001)
                     /locus_tag="VEW08_07465"
     CDS             complement(8300..9001)
                     /locus_tag="VEW08_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40610.1"
                     /translation="MIRISLFAAVLAATATLCACKPNTETPEAAEPPAEEAQGTEAAA
                     PTLALLDACKIRMTLPEAHEWETKWNPAHIQTAGQNPSNVRSAHWGDEAEKKTAREMG
                     TAIPLDLVCGNDDDVKPSIKFDITAFDSSPEEVPLQPGIYKIAPKASPAKNKPGEFIV
                     GFMGFGDSMFQATSGTLKLDRFDMNGASGSFVIDGNEILMGSRPMHIEGTFDMPCRKE
                     MLQSACQSGKAEELN"
     gene            complement(9116..>9629)
                     /locus_tag="VEW08_07470"
     CDS             complement(9116..>9629)
                     /locus_tag="VEW08_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HYJ40611.1"
                     /translation="FFTRPGMRIDLGGIAKGYSVDRGIEILQKQGITRAMVNAGGDTR
                     IIGDRFGKPWVVGVRDPDHAGKVFLRLPLSDTAFSTSGDYERYFDEDGKRFHHIIDPK
                     TGDSARKVRSVTIISGTATRTDALTKSVFIMGAEEGIAFINTLPDVDAVTVAPDGKVT
                     YSRGLAPPGQ"
BASE COUNT         1738 a         3165 c         2939 g         1787 t
ORIGIN      
        1 aatgccgacg gcaccggcat ccaccagatt tccttcaacc cgagcagcga cctctacccg
       61 caggtactgc agaacggacg cgtgttgttc acgcgctggg accgcgcccc gggccgtgac
      121 ggattgcacc tgtacacctc gaaccccgac ggcaccgaca tgcagctgca ctacggtgcg
      181 gtttcgcatc tgaccggcac cgtcgacccc accaccggcc agccgacctc catcgaattc
      241 atgcgcgcgc gcgagatgag cgatgggcgg ctcatggtgc tcacgcgcca gcgcaccgac
      301 gtcgacttcg gcggcaacct cacgatcatc gacaccaacc gcttcgtcga gaacacccag
      361 ccgctgctca cgaacgccgg catgcccggc cccgcgcaaa ctccggccac gttgaacccc
      421 gtgagcacat tgcccggccc gtcggaaggt ggacgttttc aatcgggctt cccgctttgg
      481 gatggctccg gacgcattct cgcgagctgg tcgcagtgcc gcctgctcga caccgtcgac
      541 ccgacgcgca tcgtggcctg caccgacgag cggcttgcga atccgaacgc gcaggcagcg
      601 ccgccgctgt acagcatctg gatgctgaac cccgcgcaga acaccctcct gccggtcatg
      661 cagccggtcg aaggcatcat ggtgaccgag gcagtggccg ctcagccgcg cacgccgctg
      721 ccctccgtca tcgtcgacca gcagccaccc gcgctcgatg ccgacctcgt cgccgaaggc
      781 gcgggcctgc tgtcgatccg cagcgtctac gacatcatgg gtgtcgacca ggcgcgtacc
      841 accgccggca cggccaccag catcgccaac gtttccaatc cctcgctcgc gcagtacggc
      901 agccgcctgg cgcgcttcgt gcgcatcgag aagccggtgt cgattccgga cgatgacgac
      961 atcgccgatc ctgacaactc cgcgttcggc cccacgggcg tgatgcgcga gatcgtcgcg
     1021 tacgcacccg tcgagcccga tggttcggtg cgcatgaaag tgccggcgaa cgtcgccttc
     1081 cagatctcgg tgctcgatca gaacggccgc cgcttcagcc ccatccatcg ttcctggctg
     1141 caggttcgac ccggtgaagt catcgagtgc aatggctgcc acgtgcgcaa cgccgtgaca
     1201 ccgacgacgt caccgctggt tcacggccgc aagggcctgt tcaatgccgt gaacacaggt
     1261 gcccctgccg gcccattccc cggcgccgtc acgtcaattt cgtccgacgg cggcgacacc
     1321 atggctcgcg cgcgctcacg cgccgcctcg tcgtgcgtcg acccgatcac catggtcgag
     1381 aactgcggcc ttcagtccat cacgccgagc gccaatgtcg tttacaacga gatctggaag
     1441 ccggcagcgc tgcccaccga ttcgttttcg tacagctatc aacagttgac gacggcctcg
     1501 ccggcgaatg tcggctgctt cccggtgtgg acgccgacct gccgcatcac cctgcactac
     1561 gccaccatcg gcactcgcgc gggtcagatc catcctctgt ggtccgtgcc ccggcctgcg
     1621 gccggcgtgg acaccaataa tgacggcatc ctggaatttc cgcagacctg caccaactgc
     1681 cactcgcgcg tcgatccggt ggatgcggcc gtggtgcaat tgcctgccgc ctcgctcgag
     1741 ctcaccgacg aagtgtcgga cgaagatgcc ctgcaattgc gcgcgtaccg gcacctgctg
     1801 ttcaatcggc cgcagctgga attggtcatg ggcgcgatcc agcaggtgac cattccggga
     1861 ccgccggacg acaacggcat cccctcgcag atcccgcaga cctttccgcc atcgatgggg
     1921 gcaagcaatg cgcgcggctc acggttcttt accgtgatga acaatacgac gcatgcaggt
     1981 atgctctcga ccgctgaact gcgcctgctt tcggagtggc tggatattgg agcccagtat
     2041 tacaacgacc cgttcccgcc gactccgcag aactagtctc gcgcttgctt tcgtcttcgc
     2101 gtgttctctt tcggtcacag cgttagcgaa gcagaccgag gaagaaaagg ccgccaaggc
     2161 agccgcggcg caggagaagc tgctgcaggt gttcgtcagc gagccgtacc tcgagttggc
     2221 gatcggacct ggtcgtggct atgcggtgtt ccacgtggtg gagcgcgagc agtcggtgga
     2281 cgtcctgttt cggcgcacag actggttcaa ggtgcgcacc gaacagggcg tggaaggctg
     2341 ggcccgtgcg cgcgacatgc gccgaacgaa gctcgccgac ggatctccat tcgtcttcaa
     2401 tctcggcgac cgcgccggtt tcacgacgca cgactgggaa atcggcattg gcggtggcga
     2461 ctatggcgga gcgaacctga tcgccgcgta cggttcgtac tcgctcaccg acaacatgaa
     2521 gatcgacctg tcgttgtcgc agttcctcgg caattcgtcg aatggctgga aagccgagat
     2581 cggactgcag cacgtactgt tccccgaatg gcgtttctcg ccgttcctga cgctgggcac
     2641 tggttatgtg gagaccagcc cccgcgccac gatcgtgctg ccgctcgatc gcgcggacca
     2701 gaccgcctac gccggggcag gggcacgttt ttatctcacc cggcgctttt tcttgcgcgc
     2761 cgactatcgc tggcacacgg tatttaccag ccgcgatgac aacgaggagt tggaagaatg
     2821 gaaagtcgta atcgcctgct tcttctgacg gcggtttgct gcgccacgct gatgctgagc
     2881 ggctgtgcgt cgtttcgctg gccgtggggc aaggaaaagg tcgaagaaca gcagaccgac
     2941 accgaggccg ccgaagaaga agcggaggaa caggacgtgt cgccaccacg cgtcatcgaa
     3001 ccgaacgtga cgcgccgcaa gatcaagact cccaggatcg acaacgaaaa ttgggaagtc
     3061 ggcgcaggcc tcgggttcct gtcgatcgag gactttggta cgagctccac ctacgccgca
     3121 cagcttagtt accatgtgac agaagacttc tttttccgcc tcgacgcagg gcaatccacg
     3181 gctggcgaga ccagtttcga gacgctcggc ggcgacatcg agcttctgac cggagatgag
     3241 agacgcttca cgtattacag cctctcgctg ggctacaact tccttcctgg cgaggtctat
     3301 ctcggtcgca atctcgccat gaacagtagt ttttacctgt tgggtggcat cggtagcgtg
     3361 gatttcgccg gcgacgatcg gttgacgatc aatctcggcg cgggctaccg cgtgttaccg
     3421 accgactggc tggcgataag tatcggagtt caggaccgcg tgttcgactc cgacttgtta
     3481 ggcgagacca aggtcaccaa caacatagaa atgctgctca gcgcgaccgt cttcttctga
     3541 cggagtgagc tttatgatgg cacgtacgct gaaagccaaa ggaatcggac gagtcgctaa
     3601 aggactggcc ctcgcgctgg tgttcgtgct gccggcattt gccgcatcgt cctcgggccc
     3661 cgctccggcg ttccagctcg ccgggcgcaa tggcaaggcc atcgacctct cccagttcaa
     3721 gggccaggtc gtcatgatca atttctgggc cacctggtgc aaaccgtgcc gcgatgaaat
     3781 gccgttgctc gaagacatct acaagaagta caagcccatg ggcttcacgc tggtcggcgt
     3841 gaatgtcgag ccggatgcca aggacgctga agcctggttg ggcaagctcg cgaagccggt
     3901 gagcttcccg atcgcgttcg acactgacag caaggtcagc aagatgtaca aggtagccgt
     3961 catgccgagc accgtgttcg tcgaccgcaa gggaaacatc cggatcatgc acaagggcta
     4021 caaggccggc gatgagaaca cctatctctc gcagatccgt tcgatgttga aggaataggc
     4081 caggagaatg ggagatacga ttatgcgcac ccgtggttgg ctagttctgt ccgccctcgc
     4141 cgtcttgtcg gcgggctgca gctccatcga gccgtgggtg aagccgtacg atcgcgagaa
     4201 cctcgccgat ccgatcatgg cgttcacgcg cgcacccatc tcgcaggact tcctcgacca
     4261 cgtgttcgaa tcgcgcgaag gttcgcgcgg cgccaccggc ggcggtggcg gcggctgcgg
     4321 ttgtaactaa caaagcagcc gcttgatgaa agcccactac acgcgtctcg ttttattcgt
     4381 cataacctgc ctcgccggta ccgcaacggc gggcgtgctt cccgaagatc gcgccgacct
     4441 cctctacttc cgctacgacg gcggcggcgt ggaaatcacc ggcccctcgg tgctggtgcg
     4501 caagagcatc ggcgaacact tctccgtggc cgccaactac tacatcgaca tggtgtcgtc
     4561 ggcgtccgtg gacgtggaaa cctccgccag cccctacgaa gacgaacgca cgcagtcgag
     4621 cctcgcgctc gacttcctgc acggcaagtc cacctacagc gtcggcgtgg tgaagagcga
     4681 cgaaagcgat tacgtggccg acacctggta cgcgaacgtc agccacgaca tgttcggcga
     4741 cctcaccacc atttctttcg gcttcaagaa cgggcagaac gaagtgttcc gcaacgtgaa
     4801 gatcgacggc gtgaaggtca acgaccccaa tttcgccgac gagatggagt cgaagagctt
     4861 caacgtctcc gtttcgcaga tcatcaccaa gaacctggtc atgatcggcc agtacgaggt
     4921 gatcaccgac gaaggcttcc tgcgcagccc ctatcgctcg gtgcgcttct tcatcgaccc
     4981 gttcacccag ggccagcagt ccgagatcta cccgaacacg cgctcgagca acgccgcgtc
     5041 ggtacgcgcc aaatatttcc tgccttatcg cgccgccgtc gacgccatgt accgcttcta
     5101 caccgacacc tggggcgtcc tcggccatac cggcgaactc ggctacgtgc atccgctgcc
     5161 caaccactgg atcttcgaag gccgcgtgcg ttactacgcg cagaccagtc ccgccgactt
     5221 ctaccaggac atcttcccgc gcgccgacta cgccaacttc atggcgcgcg acaaagagct
     5281 tgcgacctac gacgcgatca ccgccggcgt ctcggctacc tacgaattca agatcgaccg
     5341 gttcccgtgg ctggccaagg gcgcgctgaa cctgcgctac gattacatga cggtgaacta
     5401 cgatgacttc cgcgatgcgc gctacagcct cggaagtttc ggcacgttgc ccgatgagcc
     5461 gctgcagccg ggcactgagc cgctgtacaa gttgaacgcc aacatcatcc agttctacat
     5521 ctccgcgttc ttctgattgg gggcagcccc cttttcccgg caaaagggtc aggcgtcgcc
     5581 agccggacgc atgagcgttt ctgacatcca tcgcgcgtcg gattcctccg acatgcgtcg
     5641 ctcaccgagc gcctagcaag aatttcccgc tcgcttttca taagtgaacc tgcgactccg
     5701 cgccccgccc gcttcccgcg ggctttcgcg cacttagaca attcgctaat ggacttcgcg
     5761 ccggaaaggc cgcgctcccc gagcgtgcga gcgcaaccac tggttttccc gcgttgaccc
     5821 tcgcgcagga caggcgtatt cctgcgtttc acgcaatctt ccgtgcgtgt cgtagataga
     5881 ggggacataa cggacttttc tccttcgcgt ccggcgcccg gcgtcggcgc tttgctgcgc
     5941 cttggccagt caggaatgaa atgacgagaa ccaggatggc gttgattgcg gtggcctgca
     6001 taacagcggg cgccacagtg aatgtcgcca cggatggcgg cgggcctccg cgcgtaggga
     6061 cgcgcgcgga ggctccgcct tacgcagcgc cggtggcggc tgcgccatcc cgccctgcac
     6121 gctcaatgtc atcgttgcag ttgccgagat cttcgcccgc gcgcatcgaa gacttcgaac
     6181 gcgagcagct cccggaagtg gtcggacaag atccggaagc cgccgcgcgt ttcgccgaaa
     6241 tgcagaccga atccgaattg cgcaagctgg ccattcgcca ggtggcgcag ctctggggtg
     6301 cgctggatgc caaccgcgcg gtcaattggg cccgcacgct tcccgatgcg gccgaacgcg
     6361 acgcggcgtt catcgacatc gccaatggac tttcgagcgt cgatccgggc cgaggtgtcg
     6421 aactgcgcga gcaattcgtt gccgcgaccc ggcccgacag cgcgctcaac aatctcgtgc
     6481 agcaatgggc aggctacgac ttcgacgcag cactggcctg gacgaatgcg cgcgcgcccg
     6541 gcgcgcaacg cgacgacctg atgcagcgac tcatctacgt gcgggccgcc gcgggcaagc
     6601 cggccgacgc tgcccgcctc gtgaccgagt cgtcgttgtc aggcgcggcg cgaaccgccg
     6661 cgatcgccag cgtcgcccgg gagtgggcca gccgcgacgg cgacgctgcg caggagtgga
     6721 tacagacgct cgagccggcg accctgaact gacctcagtt ctctttcgcc ttgccgaatt
     6781 gcacgccacc gtcgtgggtg atgctcggat agaccttgcg cagctcgatc atctccgcgc
     6841 ccgccagcaa taccggcccg tagccgtgcg cggccagcac gtgcaccggg cgatacatgt
     6901 agaacatcgg atcccagccc acgcccgtgc ccacgcaggt gccttccacc tgtcctttcg
     6961 cattcaccct ggttacgacc gcgttccagc cgagcgacac ggcagggccg aacgccagcg
     7021 ggtcgagcca gccgcgattg atgccacgcg cgatcgcaaa cacgaacatc gcgcttgccg
     7081 aagtctcctc gtatgactca ctccgatcga gcagctggtg ccagcgcccc gcgtgaccct
     7141 gcacggcggt gaggcctgcg gcgtgcgcac gcagcagcgc gaggatgcgg gggcgcgcct
     7201 gatgatcctc gggcagcacc tctaacaact ccaccgtcgc catgatggcc cagccattgg
     7261 cgcgcgccca gtgaaacgcc ggatggtgct tcatgtcctg cacccaggcg tgcatgaaca
     7321 gccccttgtc cttgacgaac atgcgatccg agaactgcag gatctgtttc gccgcgtcgt
     7381 cgtaataacg gcgctcgccg gtgagttttc cggcctgcgc gaggcaggga acgctcatgt
     7441 agaggtcgtc gagccacaac gaattcggca gcgggcgatt gcgcgccagc gtgccgtcct
     7501 tcaggcgaaa ctggccggac gatacgtact gcacatagtt gtcgatccag gggcgcagct
     7561 tcctgtctcc gagtcccgca agctccgcct tgatgagagc ggcgcacatc gctcccgagt
     7621 cgtcgagcct gccgggcagc aggatggatc gcagaccgag gccgtgctgc gataccgggt
     7681 agttagcatg cgtggtgccg ggcggcaggt tcttcttcgc atgagccgcc agcgtcgcaa
     7741 tggcgaggac tcgctggtgc gtgtagtcgc tgtagcgttt gtcgccggtc acctgcgcgc
     7801 tgcgcaacat gccggcatag gtgactcccc actcgtaggt gaggatctgc atgtcggtgc
     7861 gcgccagcgc caccctggtg ggcaggttcg tgagatccgt gacgggcgcc cccgtctccc
     7921 cgtctatcac ctgcaccgga gcggcctggt ccacgtacgc atgcacgcgt tcgagcgtgg
     7981 cgcggatctg ttcgacggtg gcgggctcat aaggcgtggg gtagtccacc ttgaggcggc
     8041 cggaatacac accggggtcc tgctgcggcg gtgtatgtat ctgcgtcggc gtggattgcg
     8101 cgagagccgg atgggagcaa cacaccgcgg ccgtcagcgc gaacagaagg taattcgcgg
     8161 cgcgaagctg cggggtcgac gtgtgaggca tggagttgtt cctgaagtta ccgcgatgca
     8221 gatcgcgcgg aacaatagct tcaagcaacg tcgctgcata gcgccgcgcg acccgctcgc
     8281 gcggcgctaa caatacgcct cagttcagct cttcggcctt gcccgactgg caggcgctct
     8341 gcagcatttc ctttctgcat ggcatgtcga acgtgccttc gatgtgcatc gggcggctgc
     8401 ccatgaggat ttcattgccg tcgatgacga atgaaccgga ggcgccgttc atgtcgaagc
     8461 ggtcgagctt cagcgtgcca ctggtggcct ggaacatgct gtcgccgaaa cccatgaaac
     8521 caacgatgaa ctcgccgggc ttgttcttcg cgggactcgc cttgggggcg atcttgtaga
     8581 tgccgggctg cagcggcacc tcttccggtg aggagtcgaa cgccgtgata tcgaacttga
     8641 tcgacggttt cacatcgtcg tcgttgccgc agaccaggtc gagcgggatg gcagtgccca
     8701 tctcgcgtgc ggtctttttt tccgcctcgt cgccccagtg cgccgatcgc acgttactcg
     8761 ggttctggcc cgccgtctgg atatgtgcgg gattccattt cgtttcccat tcgtgcgctt
     8821 cgggaagagt catcctgatc ttgcaggcgt ccagcagcgc gagagtgggc gctgcggcct
     8881 cggtgccttg cgcctcttcc gcgggtggct ccgccgcttc cggcgtttcg gtgttcggtt
     8941 tgcaggcgca aagcgtggcg gtcgccgcca gcactgcggc gaaaagactg atacggatca
     9001 ttcgagacct ctgggttaag gcgcactccc ttgtgcgcgc ggcgaatgct cccactccac
     9061 gggagtcagc accaatgtcg gtagctagcg acgtattcgc tagttagagg cggtctcact
     9121 gcccgggcgg cgccagtccc cgtgaataag tcaccttgcc atcgggagcc acggtgaccg
     9181 cgtccacgtc cggcagcgtg ttgatgaacg cgatgccctc ttccgcaccc atgatgaaga
     9241 cgctcttggt cagtgcgtcg gtacgcgtgg ccgtgccgct gatgatcgtc acgctgcgca
     9301 ccttgcgcgc cgagtccccg gttttcggat cgatgatgtg gtggaaccgc ttgccgtctt
     9361 catcgaagta gcgctcgtag tcgcccgagg tcgagaaggc ggtgtccgac agcggcagcc
     9421 gcaggaacac cttgcctgcg tggtcgggat cgcgcacgcc gactacccag ggcttgccga
     9481 aacgatcgcc gatgatgcgc gtgtcccctc cggcgttgac catggcgcgc gtgatgcctt
     9541 gcttctgcag gatctcgatg ccgcgatcga ccgagtaacc cttggcgatg ccgccgaggt
     9601 cgattcgcat gccgggacgc gtgaagaac
//
LOCUS       DASQJS010000177         4763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_409462, whole genome shotgun sequence.
ACCESSION   DASQJS010000177 DASQJS010000000
VERSION     DASQJS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409462"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VEW08_07475"
     CDS             complement(<1..1252)
                     /locus_tag="VEW08_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like protein"
                     /protein_id="HYJ40612.1"
                     /translation="MNESRISREALIHQLYEAAELEHNLMCTYLYAAFSLRSGTEEGL
                     SAEEADATARWRRAILKVAIEEMGHLTAVWNITAALGGSPRFGRLNFPLDPGGLPANV
                     VVRLAPFSEEVLQHFIYLERPLGSAEPDGAGFEADFKFTRGDSRPRVTPMPIDYDTVG
                     AFYETLSGNVRAFVARVGEKEAFCGDRNLQLSRKEIDFQGCDPVICSITALKAFDAIV
                     SQGEGASTENADSHYCRFKSIREELKALRAKNPDFRPAFPAAVNPVLRRPVRAGARVW
                     LEHEETAATVDLANTAYMLMLRLISHSYLLPRPHPAKALCVDLGLGLMRVMSPLAERA
                     ARLPAGPSNLDCNGGMSFTALRDAAPLPPGISAGKFFLERLRELLAGMQALAANGDAR
                     VAQALKILNDLAQRAERTFANIEKD"
     gene            1772..4429
                     /locus_tag="VEW08_07480"
     CDS             1772..4429
                     /locus_tag="VEW08_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ40613.1"
                     /translation="MGINRDLRPLRNLGYAFALFATCAAIVLAAPAHQSPTLHTAFDG
                     GIFVLTGVLALLILDMAWRANDGFAKLLAVAFAIAAAFELVHVAAAVEYSANPDEVAA
                     IAARLRPSTWPPATYALPLGLIAAFVLRTRAGRTDLVLAAGLSAAGLALAVIFNFIPP
                     YAPPGLFGMTRPSLVLVPMMWIIVLLLYWPLRVHERLARYIALFSILAIVSNVAMLYS
                     AYPADDPALLAHAGKFANRMFLLFTLMQIGTLETARRIQGERDLTRLNDALEDRVHDR
                     TVDLQAANLALRSEAETRANAEAKALAQLRRLRLLQQITHAIAERQDLRSIFQVVVSS
                     LEDHLKVEFTLFCNYDAVDKQLSVAQVGPKSTFIAQQLGLCEGAKVEIGSNGLSECVR
                     GRLVYEADLSLLDFQFSQRLAAAGLRSLVAVPLVVEQRSGVFGVLIVARNEPAGFGSD
                     DCEFLAQLGENVALASNQTQLNVALQHAYDELRSTQHAIMQQERLRALGQMASGIAHD
                     INNAISPATLYLDSILVRDTLLEPGTRRKLETIQRAIGDVAQTVARMGEFYRQRDAQL
                     KPTPVNINVVLAQVPELTRARWSDVALAAGTSIKLKVEVAPDAPTIMAIESEIREALI
                     NLVFNAADALKTGGTIILRARRASANPHSGPQRVVVEVCDDGMGMDDEVRSRCLEPFF
                     TTKGERGTGLGLAMVYGIAQRHRAELQIDSESGKGTTVRLIFPDDEVMEHVERERSVA
                     ADAPKLKILLIDDDPLILHSLRDALAYEGHNVACANSGQTGIDMFNGAHDRKEPFAAV
                     ITDLGMPHMDGRQVAAAIKAISGSTRVIMLTGWGQRLAENGETAGRADTILSKPPNMW
                     DLRDALYDVVRASVQEPLKARTVPLDRVG"
     gene            complement(4490..>4763)
                     /locus_tag="VEW08_07485"
     CDS             complement(4490..>4763)
                     /locus_tag="VEW08_07485"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HYJ40614.1"
                     /translation="LAIGKPVGFILASWLAVKLGIAEKPAAYSWRQLAGAGALAGIGF
                     TMSLFIAGEAFPVMDDFAAAKIAVFAASVLSAVAGVAILWGAKPPE"
BASE COUNT          880 a         1550 c         1498 g          835 t
ORIGIN      
        1 aatctttttc gatgttcgca aacgtgcgtt cggcgcgctg tgcgaggtcg ttgagaatct
       61 tgagcgcttg cgccacgcgc gcatcgccat tcgccgccag cgcctgcatg cccgccagca
      121 attcgcgcag ccgctcgaga aagaactttc cggcactgat gccggggggc agtggcgcgg
      181 catcgcgcag cgccgtgaat gacatgccgc cgttgcagtc cagattcgac gggcccgcgg
      241 gcagccgcgc ggcgcgctcg gcgagcggcg acatgacgcg catgaggccc aggcccaggt
      301 ccacgcacaa tgccttagcg ggatgcgggc gcggcaacag atacgaatgc gaaatcagcc
      361 gcagcatcag catgtacgcg gtgttggcga gatcgaccgt ggccgcggtt tcttcgtgct
      421 cgagccacac gcgcgcgccc gcgcgtaccg gccggcgcag cacgggattc acggccgccg
      481 gaaacgcagg ccggaaatcc ggattcttcg cgcgcagcgc tttcaattct tcgcggatgg
      541 acttgaagcg gcagtagtgc gagtccgcgt tctccgtgga ggctccttcg ccctggctga
      601 caattgcgtc gaacgccttg agcgcggtga tcgaacagat gaccgggtcg cagccctgga
      661 agtcgatttc ctttcgcgac agctgcaggt ttcggtcgcc gcagaacgcc tctttctcgc
      721 cgacgcgtgc gacaaaagcc ctcacgttgc ctgacagggt ttcgtagaac gcgccgacgg
      781 tgtcgtagtc gatgggcatc ggcgtgacac gcggccgcga atcgccgcgc gtaaatttga
      841 agtccgcttc gaagcccgcg ccgtcgggtt cagccgagcc cagcggccgt tcgaggtaga
      901 tgaagtgctg cagcacctct tcgctgaacg gcgccagtct aactactaca ttggcgggca
      961 ggccgcccgg gtcgagcgga aaattcaacc gtccgaatcg cgggctgccg ccaagcgcgg
     1021 ccgtgatgtt ccacaccgcc gtgagatgcc ccatctcctc gatggccacc ttgagaatcg
     1081 cgcggcgcca gcgcgcggtg gcgtcggctt cttccgcact caatccttcc tcagtgccgc
     1141 tgcgcaggct gaatgccgcg tacaggtacg tgcacatgag gttgtgctcg agctcggccg
     1201 cctcgtacag ctggtgtatg agcgcctcgc gggaaattcg tgattcattc atgatttcga
     1261 gagcgtactg gcccagtacc gaagagggaa gtcgtcgcac gaaccccgat gccatcgtct
     1321 tcgactcgtg gccaccggtt ttgtggcaag aagggggcat ggacaaggcg aaccgcgtgg
     1381 ctatcgagcg ccccgcgtac gccgggatgc gcattgacgc aaaagagggc attcgcgtga
     1441 tcgatgtggc gctccagtgc atcgcacccg gcgtgcctgg gatgcgcttc ccctcgggtc
     1501 atttgccgcg accggtgcgg ggaccgcgcg ggcgatcctt taccgcaaac tgacgggccg
     1561 cggaatacgt atcgaacgcg aaacctgtgc tttcgccggg gttgtttgtc ctgcgcctct
     1621 cttgcccggc agcccttacg tatgagctca tcacaatcaa ccacggggcg cgcaccgata
     1681 ttagttagac atgtagtgcc agcgccggcg caggagtacg cttcaccgac agaatcagaa
     1741 ttcgccgcca tgcaggacgg gagatcatcg catgggcatc aatcgcgacc ttcggccgct
     1801 gcgcaacctc ggctacgcat tcgcgttgtt cgcgacgtgc gcggccatcg tgcttgctgc
     1861 acccgcacac cagtcgccaa ctctgcatac ggccttcgat ggcggcatct tcgtgctgac
     1921 cggcgtgctg gccctgctga tcctcgacat ggcatggcgg gcgaacgacg gattcgcgaa
     1981 actcctggcc gtcgcgttcg cgatcgccgc cgcgttcgag ctcgtccacg tggccgcggc
     2041 agtcgaatat tcggcaaatc ccgacgaagt tgcggcaatc gccgccaggc ttcgaccttc
     2101 gacatggccg cccgccactt atgcgctgcc actcgggttg atcgccgcgt tcgtgctgcg
     2161 cacgcgggcc ggacgaacgg atctcgtact ggcggccggc ttgtcggcag ccgggctggc
     2221 gctggccgtg atcttcaatt tcatcccgcc ctacgcaccg cctggcctgt ttggcatgac
     2281 ccggccgtcg ctcgtgctgg tgccgatgat gtggatcatc gtgttactgc tctactggcc
     2341 gcttcgcgtg cacgaacgcc tcgcgcgcta catcgcactg ttttcgattc tcgcaatcgt
     2401 cagcaatgtc gcgatgctct actcggctta tcccgcggac gaccccgcat tgctcgcgca
     2461 cgccgggaaa ttcgccaacc gcatgttcct gctgttcacg ctgatgcaga tcgggacgct
     2521 cgaaaccgcg cgaaggattc agggtgagcg cgatctcacg cgtctcaacg atgcgctcga
     2581 ggaccgcgtc cacgacagaa ccgtcgacct tcaggcggcc aatttggcgc ttcgatccga
     2641 agccgagacg cgcgcgaacg cggaagccaa ggcacttgcg cagttgcggc gcctgcggct
     2701 gcttcagcag atcacgcacg ccatcgccga gcggcaggat ctccgcagca ttttccaggt
     2761 cgtcgtgagc agcctcgagg accacctgaa agtggaattc acgctgttct gcaactacga
     2821 cgccgtcgac aaacaactgt cggtggcgca ggtcggcccg aagagcacgt tcatcgcaca
     2881 gcagctcggg ctctgcgagg gtgcgaaagt cgagatcggc agcaatggcc tgagcgaatg
     2941 cgtgcgtggc cgcctcgtct atgaagcaga cttgtcgctg ctcgacttcc agttctcgca
     3001 gcggctcgcc gctgccggcc tcaggtcgct cgtggcggtt ccgctggtcg tggagcaacg
     3061 cagcggagtc ttcggggtac tgatcgtcgc gcggaacgag cccgcaggtt tcggcagcga
     3121 tgattgcgaa ttcctcgccc agctcggcga gaacgtcgcg ctcgcgagca accagacgca
     3181 actgaacgtt gcgctccagc atgcgtacga cgagctgcgc agcacgcagc atgccatcat
     3241 gcagcaggag cgccttcgcg cgctcgggca gatggcgagc ggaatcgcgc acgacatcaa
     3301 caacgccatc tcccccgcga cactctacct cgattcgatc ctggttcgag acacgctgct
     3361 cgagccgggg acgcgccgga aactcgaaac gatccagcgc gccatcggag acgtggcgca
     3421 gaccgtggcc cggatgggcg agttctatcg ccagcgcgac gcccagctga agccgacacc
     3481 cgtcaacatc aatgtggtcc tggcgcaggt gcccgagctc acgcgtgcgc gctggagcga
     3541 cgtcgcattg gcggcgggca cctctatcaa actgaaggtc gaggtggcgc ccgatgcgcc
     3601 gacgatcatg gccatcgaaa gcgagattcg cgaagcactg atcaatctcg tgttcaacgc
     3661 cgccgacgca ttgaaaaccg gaggcactat catcctgcgc gcgaggcgcg cgtctgcgaa
     3721 cccgcattcc ggtccgcagc gcgtggttgt cgaagtttgc gacgacggca tgggcatgga
     3781 tgacgaggtc cgcagccgct gcctcgagcc cttcttcacc acgaagggcg agcgcggcac
     3841 cggcctcggt ctggcgatgg tctatggaat cgcgcagcgc catcgcgccg agctgcaaat
     3901 agacagtgag tccggcaagg gaacaaccgt tcgcctgatt tttccggacg atgaagtgat
     3961 ggagcatgtc gagcgcgagc gaagtgtcgc ggccgatgcc ccgaagctca agattctgct
     4021 catcgacgac gatccgctga tcctgcattc actccgcgac gcgctcgcat acgaaggcca
     4081 taacgtggcc tgcgcaaata gcgggcaaac cggaatcgac atgttcaatg gcgctcacga
     4141 tcgcaaggag ccgtttgccg ccgtcatcac cgatctcggc atgccgcaca tggatgggag
     4201 gcaggttgcg gcggcgatca aggcgatatc cgggagcaca cgagtgatca tgcttaccgg
     4261 gtgggggcaa cggctggcgg aaaacggcga aacggccggg cgggcggaca cgattctcag
     4321 caaaccaccc aacatgtggg acctgcgcga tgcgctctac gatgttgtcc gcgcgtccgt
     4381 gcaggagccc ctcaaggcca gaactgttcc tctcgatcgt gtgggctgaa aagccaggca
     4441 agaacggcgg aaacaagttg cccggccggc gcggtgagcc ggcagacgtt cactccgggg
     4501 gcttcgcgcc ccagagaatc gcgaccccgg ccaccgccga cagcaccgac gccgcgaaca
     4561 ccgcaatctt cgccgcggca aaatcgtcca tcaccgggaa cgcctcgccg gcgatgaaca
     4621 gtgacatcgt gaatccgatg cccgccagcg caccggcgcc ggccagttgc cgccaggagt
     4681 acgccgcagg cttctcggcg atgcccaact tcaccgccag ccacgacgcc agaatgaacc
     4741 cgacaggttt tccaatggcg aga
//
LOCUS       DASQJS010000178         4780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2611580, whole genome shotgun sequence.
ACCESSION   DASQJS010000178 DASQJS010000000
VERSION     DASQJS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2611580"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4289)
                     /locus_tag="VEW08_07490"
     CDS             complement(<1..4289)
                     /locus_tag="VEW08_07490"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HYJ40615.1"
                     /translation="MIRIRVPALAVMAAAFLLVGCSGNDSVATRPNVTAPDPQWVTSI
                     SQHSNGAISRHSPVRVLFTSDVVPQERVGSSASANITITPAVKVTATFASRREIVLRA
                     TPEFAPGTDYRISVRAQGLTGVPADTKPFEFTVKTLDVNFDVRTYGLDVEQDRNELMV
                     LRGAILTADSEDRERIEKIVTATLDGKPLVLVWTAGEHEHRFTINDIVRKREEQEVVL
                     KWDGAPLNLRNAGSQSWRIPGLGEFAVTQAQAVQQNDQRQVQVRFSDSLDARQDLKGR
                     VRLSQGEFTTSINSNLLTVYLNEDVVGEVTLTLEAAIRSRSGQALVGTREFKLEFTST
                     RPQVRFVGTGVILPDAKTLSVPFEAVSARAVRVTALQVYEANIPQFLQVNALSGSQEL
                     GRVGRVLWRKTIPLASPVPGKWTRYDLDVTELTAKHPGGLFQLTLSLAPADALYDCPG
                     STETASPEPDPASQDGNDTADYSNWDYYSEEYYEGEINWNERDDPCKPAYYRYGRNIR
                     AARNLLASNIGLIAKRAQRGRLLAVATALDTAKPLAGVKVDAMSYQNQVLASGRTDNN
                     GMVELDPRGTPFALIADDGGRKGYLRVANGAALPVSHFDVGGETVIAGLKGHFYGDRG
                     VWRPGDTIHLTFALQDRTHTLPPDHPVTVELRNPRGQLVQTLINSKPVGQFYTFELKT
                     AADAPTGDWNAVALVGGATFGKTLKVETVMPNRLKIDLDLGEKPVIESSPLKGSLNAQ
                     WLSGATAASLRAAVEVRLSSAPTRFTRNADYVFDDPARSFQGAPITVFDGELDAEGKA
                     RVEKDLDLPRDAPGMLNATFITRVFERGGAFSINRETRTVAAFDRYVGLKLPKGDAAR
                     DMLLTDTRHVVELATLDADGAPVSVPRLQVTIYKVQWRWWWESGGESLAQYAQGESTS
                     QIKQGTIASKDGRAQWDFEIKYPEWGRYLVRACDLDGGHCTGRVFYVDWPSWAGAARD
                     QSGPAANILMLTSDKQEYRVGETATVQLPEASQGRALLTLESGSAILEQRWLEAKPGA
                     NRVSIPITAGMAPGIYAAVTMVQPHAGKTNDRPIRLYGVIPLKVTDPQSHLAPLVTTA
                     AEWAPQSKASVTVSEKAGRAMDYTLAVVDEGLLGLTGFRTPDLHAEFYRREALGVSTW
                     DLFDEVAGAYGGQLDRMLALGGSDASAATNPDDGKSRFPPVVRFLGPFSLKAGEKRAH
                     SVQLPQYVGAVRVMVVAGDGSAYGSADKSVFVRQALMILPTLPRVIGPDEQFSLPVSV
                     FTSEASIKSVKLEVQTDARFSAVGSAAQQIAFTRPEEKLGFLTLRSGSALGKGKIHVV
                     ATSGRYRAESDVWLEVRAPNVPSSRYQRAMLAPGQSWTGDLAAFGLPGTQVANLEVSA
                     LPPVNLDGRLEYLIRYPYGCLEQTTSSVFPQLYLPALIRLDQNRRLEVENN"
BASE COUNT          785 a         1463 c         1627 g          905 t
ORIGIN      
        1 atgttgtttt ccacctcgag ccggcggttc tggtcgagtc tgatcagcgc cggtagatag
       61 agctgcggga atacgctgga ggtggtctgc tccaggcacc cgtacggata gcgaatgagg
      121 tattccagcc gtccatcgag attgacaggc ggcagcgccg agacttccag gttcgccacc
      181 tgggtccccg gcagtccgaa agcggcgagg tctcccgtcc aggattgccc gggcgccagc
      241 atcgcgcgct gatagcgcga ggagggtaca ttcggcgcgc gcacttcgag ccacacgtca
      301 ctttccgcgc gatacctgcc gctggtggcg accacgtgga tcttgccttt gcccagcgcc
      361 gagccggagc gaagcgtgag gaacccgagc ttttcttcgg gacgcgtgaa agcgatctgc
      421 tgggcggcgc tgccgacggc gctgaaacgc gcgtcggtct gcacctcgag tttcacggac
      481 ttgatcgacg cctcgctggt gaacaccgag accggcagcg agaactgttc gtccggtccg
      541 atcacgcgcg gcagggtggg caggatcatc aacgcctggc gtacgaacac cgacttgtcg
      601 gcagagccgt aggcgctgcc gtcaccggcc accaccatca cgcgtaccgc tcccacgtat
      661 tgcggaagct gcacgctgtg ggcgcgtttc tcgccggcct tgagcgagaa gggcccgagg
      721 aaccgtacca ccggcggaaa tcgcgacttg ccgtcgtcgg ggttggtggc ggcggaagca
      781 tcgctgccgc ccagtgcgag catgcggtcg agctgcccgc cgtaggcacc cgcgacttca
      841 tcgaacaggt cccaggtgga gacacccagc gcttcgcgcc ggtagaactc ggcatgaaga
      901 tccggcgtcc tgaagccggt cagtcctaac aacccttcgt cgaccacggc gagcgtgtag
      961 tccatggcgc ggccggcttt ttcactgacg gtgacgctgg ctttcgattg cggtgcccat
     1021 tcggcggcgg tggtgacgag gggagcgagg tgcgattgcg gatcggtgac tttcagagga
     1081 atgacaccgt agagccggat cggccgatca ttggtcttgc cggcgtgcgg ctgaaccatg
     1141 gtgaccgcgg cgtagatccc gggcgccatg cccgcggtga tcggaatgga tacgcgattg
     1201 gcgccgggtt tcgcttcgag ccagcgctgt tcgagaatgg cgctgccact ttcgagtgtc
     1261 agcagcgcgc ggccctggga agcctcgggc aattgcacgg tggcggtttc gccgacgcgg
     1321 tattcctgct tgtcggaggt aagcatgaga atgttggcgg cgggacctga ctggtctcgc
     1381 gccgcgcctg cccacgatgg ccagtcgacg tagaagacgc ggccagtgca atgcccgccg
     1441 tccagatcgc aggcccggac caggtatcga ccccattccg ggtacttgat ctcgaaatcc
     1501 cattgggcgc gtccatcctt cgaggcgatg gtgccctgct tgatctggga agtgctttcg
     1561 ccctgcgcgt actgcgcgag cgattccccg cccgactccc accaccagcg ccactgcacc
     1621 ttgtagatag tgacctggag gcgtggcacc gacaccggcg cgccatcggc gtccagcgtg
     1681 gccagctcga ccacgtgcct ggtgtcggtg agcagcatgt cgcgcgccgc gtcgcccttg
     1741 ggcagcttga gtcccacata ccggtcgaac gccgccacgg tgcgcgtttc gcgattgatg
     1801 ctgaaggcgc cgccccgctc gaacacgcga gtgatgaaag tggcattgag catgcccggc
     1861 gcgtcgcgtg gcagatcgag atccttctcg accctcgcct tgccttcggc gtcgagctca
     1921 ccgtcgaaaa ccgtgatcgg tgcgccctgg aatgagcgcg ccggatcatc gaacacatag
     1981 tcggcgttac gcgtgaagcg cgtgggcgcg ctcgacaggc gtacttcgac tgcggcacgc
     2041 aaggatgctg ccgtggcgcc ggacaaccac tgcgcattca gcgaaccctt gagcggcgag
     2101 ctttcgatga ccggcttctc gccgagatcg aggtcaatct tgagacgatt gggcatcacc
     2161 gtctcgacct tcagtgtctt gccgaaagtg gcgccaccga cgagggcgac tgcgttccag
     2221 tctccggtgg gtgcgtccgc ggcagtcttg agctcgaaag tgtagaactg cccgacaggt
     2281 ttggagttga tcagcgtctg caccagttgt ccgcgtggat tgcgcagctc gacggtcacc
     2341 gggtggtcag gcggaagcgt gtgtgtccgg tcctgcaacg cgaaggtgag gtggatagtg
     2401 tcgcctgggc gccagacgcc gcggtcgccg tagaagtgcc ccttgagtcc cgcgatgacg
     2461 gtttcgccac ccacatcgaa gtgactcacc ggcaaggcgg caccattggc gacgcgcaga
     2521 tagcccttgc gaccgccgtc gtcggcgatg agcgcgaacg gcgtgccgcg cgggtcgagc
     2581 tccaccatgc cgttgttgtc ggtgcggccg ctcgccagca cctggttctg gtaactcatg
     2641 gcatcgactt tcacgcccgc cagtggtttg gcggtgtcga gcgccgtggc gacagcgagc
     2701 agcctgccgc gttgcgcacg cttggcgatc aggccgatgt tggaggcgag caggttgcgc
     2761 gcggcgcgga tattgcgtcc gtagcggtaa tacgccggct tgcagggatc gtcgcgttcg
     2821 ttccagttga tttcgccttc gtagtactcc tcgctgtagt agtcccagtt ggagtagtca
     2881 gccgtgtcgt tgccgtcctg gctcgccgga tcgggttcag gagaggcggt ttcggtactg
     2941 ccagggcagt cgtaaagcgc gtcggcgggc gcgagtgaca gcgtcagctg gaacaagcca
     3001 cccgggtgtt tggcggtgag ctcggtcacg tcgaggtcgt accgcgtcca cttgcccgga
     3061 accggtgagg ccagcggaat ggtcttgcgc cacaacacac gacccacgcg gccgagttcc
     3121 tgcgagccgc tgagagcatt gacctgcagg aattgcggga tgttcgcctc gtatacctgc
     3181 agcgcggtca cgcgcacagc gcgtgcgctc acggcttcga agggcacgct gagagttttg
     3241 gcgtcgggca ggatcacacc cgtgccgacg aagcgcacct gtggccgggt gctggtgaac
     3301 tcgagtttga attctcgagt tccgaccagt gcctggcccg agcgactgcg gatggcggct
     3361 tcgagcgtga gcgtgacttc gcccacgacg tcttcattga gatagacggt gagcaggttg
     3421 ctgttgatgg aggtggtgaa ctcgccctgc gacaggcgga ctcgtccctt gagatcctgg
     3481 cgcgcatcga gcgaatcgga gaagcgcacc tggacctggc gctggtcgtt ttgctgaacc
     3541 gcctgggcct gtgtgaccgc gaattcgccc aggcccggaa tgcgccagct ctgcgaacct
     3601 gcgttcctga ggttgagtgg agcgccgtcc catttcagca ccacctcctg ttcctcacgc
     3661 ttgcgcacga tgtcgttgat ggtgaagcga tgctcgtgtt cgcccgccgt ccagaccagg
     3721 accaggggct tcccgtcgag cgtcgcggtg acgatcttct cgatacgctc gcggtcctcg
     3781 gaatccgcgg tgagaatggc gccgcggagc accatcaatt cgttgcgatc ctgctcgacg
     3841 tcgagcccgt aggtccggac atcgaaattc acgtccagcg tcttcaccgt gaactcgaag
     3901 ggcttggtgt cggccgggac ccccgtgagt ccctgggcgc gcacgctgat ccggtagtcc
     3961 gtgcccggcg cgaattcggg cgtggcgcgc aatacgattt cgcggcggct tgcgaacgtg
     4021 gcggtcacct tcacggctgg ggtgatggtg atgttggccg acgcactgga gccgacgcgc
     4081 tcctgcggca ccacgtcgct ggtgaacagc acgcgaaccg gtgagtgacg cgagatggcg
     4141 ccgttgctgt gctggctgat cgaggtcacc cattgtggat cgggtgcggt cacgttcggg
     4201 cgggtggcaa cgctgtcgtt gccgctgcag cccaccaaca gaaaagccgc cgccatgaca
     4261 gccagggcgg ggacgcgaat tcgaatcatg gaagctccga cgggaggggt ggatcgaggc
     4321 agcgtcagtt caacgcagga agctgtcgat tgcgtgcggc aattgtggca aatgaagacg
     4381 cggtttgggg gggtgcgggc cccagcgagc agtccgcgca caggatttcg ttcgcttttc
     4441 gccgggccct tcgttcgggg tggcatcgcc agattgcggg ccgccgccac acgcaatgac
     4501 actttcgtgc ggttggagtt gtaaggcggc gagagtccgc atgaattaga gaggcggctt
     4561 cgtgcctaac gaggccaggt atcccggccc gctctctgag ctcctgtgcg cggactgctc
     4621 gctggggctc atacgtcacc gaaccgcgcc ccgcttttcc gcggcggtgg taagagaaga
     4681 gacgcacgga cttgaggtct tcccacagct tggggttaca cgcctctttt tctggcgaac
     4741 ggagcggcga cgcgtcggtg cacgatcgag cgacgggtgg
//
LOCUS       DASQJS010000179         6944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2611935, whole genome shotgun sequence.
ACCESSION   DASQJS010000179 DASQJS010000000
VERSION     DASQJS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2611935"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /locus_tag="VEW08_07495"
     CDS             complement(<1..1769)
                     /locus_tag="VEW08_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HYJ40616.1"
                     /translation="MRAPVRRALIYRRFGASLVALSLLFATTLAAPARAEKLTLERLF
                     AAPDLSGPTLRGVEISPDGRLVAYLKAREDDKDRFDLWAFDVRLSRDRLLVDSRTLAG
                     ADRALSAEEESRRERQRTSAYSGIVEYSFAPDSRHLLIPLNGDLYVYDLGRKPADAVR
                     RLTNTDAFETDARFSPHSSYVSFVRDQNLHVIDLATGKERAVTSKGGGAVSFGMAEFI
                     AQEEMDRDTGYWWSPDERHIALARVDESQVAEVERFEIQASGARVMRQRYPATGARNA
                     RVDLFVADLAAESRLQLDLGAETDIYLPRVDWFPDSRGIAVQRQSRDQKTLELMRFDA
                     LSGRGRVLLTERSEHWVPLHRELTFLQHSPQFIWASSRSGYQHLYLYGNGGNLIRQLT
                     SGEFMVVGEPPEAAIRSVDERARRVYFTANQPSPVERQLFWVSLDEPAGAQRVTAGAG
                     WHSIAMSQDARVFVDTFSEANLPRNVTLRTSGGRRLSAMLPNQLDAAHPYGRFLDEHV
                     RPEFGTFAAADGQAMHYKLLKPGTLEAGKRYPVLIEVYGGPGVQRVTNSWGSLFHQYL
                     VQHGYVVFVLDNRGSGMRGERFET"
     gene            1815..4010
                     /locus_tag="VEW08_07500"
     CDS             1815..4010
                     /locus_tag="VEW08_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYJ40617.1"
                     /translation="MLNNPRHLGALVATTLAALIAACAMSPKAQHTPVPSPAAISSFQ
                     YPAAVRGEVVDTYNGVSVPDPYRWFEDAMAPATRDWVTAENALSQPYLEKLPQRAWLG
                     ERLKQLWTYERFGVPQREGDKYFYMRNDGTQDQSVLYVADALNATARVLVDPNGKRDD
                     ATIALSQWVPSPDGNLLAYALSDGGTDWNTWHFRRVADGTDLPVILKFSKFYAVSWAR
                     DSSGVYYSRYPLKPGQKAEEAERGDDAGRPDVYFHKLVEAQSADRLVYQVTDHPTRVP
                     SGQVTEDGRYLVIGLFDGYESNAILVQDLRKPDAKPQPVFTAWDALYNFIGAKGDELY
                     FQTTNNAPRGRVIAVNANDPAPARWRTIVPHGDIAMANVSYIGGRVIVEYSRDARSVV
                     RLFEVSGAQAGEVKLPGLGTATAFQGTGANPEAFFSYSDYLSPTRILRLDVASNTVSE
                     FRTPKVPADFSPYMTEQVFYPSKDGTRVPMFITRRKDAPRDGNRPVMLYGYGGFNVTL
                     SPSFSPSIQSWLEMGGVYAVANLRGGGEYGEEWHQAGTRLKKQNVFDDFIAAGEYLIR
                     EKYTNPKRLAILGRSNGGLLVGAALTQRPDLFGAALPGVGVMDMLRYHTASANARQWS
                     SDFGISEDSAEFKALHAYSPVHNIKTGTCYPPTLVTTADRDDRVVPWHSYKFAAEMQR
                     VQACANPVLIRIETRAGHGAGKPVWMQIEDIADQFGFVANALGMPAPGG"
     gene            complement(4098..4994)
                     /gene="zapD"
                     /locus_tag="VEW08_07505"
     CDS             complement(4098..4994)
                     /gene="zapD"
                     /locus_tag="VEW08_07505"
                     /inference="COORDINATES: protein motif:HMM:NF003656.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HYJ40618.1"
                     /translation="MSAEDNELPPIVFTEGAAVAQNSPMSEEAQKTSDQQHESAAAAA
                     VAPLIFEQPLNERMRTFLRLDFLYSQALYHNEMQSQWGSRAAMGSLLDILAIAARGDV
                     RADVQKELERHLAQLNVFQSKPGVDSARLRTVMSNLLRLRADLVACGSNYLAGLKDSE
                     FLSAIKHRSAIPGGTCEFDLPDFFFWLNQATDARARSFNDWLARLRPLCDAIAELLWI
                     TRQHGKPRQEIARGGVYHITFERDTPIQLVRIALPADAGLYPETSGSHYRCSVRLLAW
                     NGLQTRPVQTTDDVPFTLTCCT"
     gene            complement(4991..5617)
                     /gene="coaE"
                     /locus_tag="VEW08_07510"
     CDS             complement(4991..5617)
                     /gene="coaE"
                     /locus_tag="VEW08_07510"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HYJ40619.1"
                     /translation="MHGGFRIGLTGGIASGKSTVANLFAALGVPIVDTDLLAREVVAP
                     GSPLLRKITDHFGMQVQGADGSLDRQELRKRIFEDPAQRKWLEALLHPAIRELTDVRC
                     EAATGPYVIVAIPLLVETGGEARFDRVLVVDCEPELQLARLTARDGSTHEEARRMLAA
                     QAPRAARLAAADDVIHNDGDIAQLRDQVEKLHRQYVAAAKAQSDKAGS"
     gene            complement(5626..6519)
                     /locus_tag="VEW08_07515"
     CDS             complement(5626..6519)
                     /locus_tag="VEW08_07515"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004710834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HYJ40620.1"
                     /translation="MELLAPLADSPALFITCTVLLGLLVGSFLNVVIYRVPVMMDNAL
                     RAECESLANPDAPPPPAFNLVTPRSACPKCKAPITALQNIPVVSWLALGRKCANCKTP
                     ISARYPLIELFTGLASGLIAWRFGYGTLALAGLFFTWSLIALTMIDFDTQLLPDDLTY
                     PLLWVGLLLSTTHPVWSQGAAPVTPADSIVGAMCGYLSLWTVYWIFKLITKKEGMGYG
                     DFKLFAAFGAWFGWRMLLPIILFASLVGCVAGVYIIYRQRKGMDTPIAFGPFLAAAGW
                     LFLLVGHQTVDRYLALYASPP"
     gene            complement(6615..>6944)
                     /locus_tag="VEW08_07520"
     CDS             complement(6615..>6944)
                     /locus_tag="VEW08_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYJ40621.1"
                     /translation="SGACGNIVFENAVLKMRDEVATGQRLQRSMENTGLFPNMVIQMI
                     AVGEEAGALDAMSGKVATFYEAEVDNAVDSMSALLEPLIMAVLGVLVGGMVIAMYLPI
                     FKLGAVV"
BASE COUNT         1263 a         2210 c         2259 g         1212 t
ORIGIN      
        1 gtctcgaagc gttcgccgcg catcccgctg ccgcgattgt cgagcacgaa gacgacatag
       61 ccgtgctgca ccaggtattg atgaaacagg ctgccccagg aattcgtgac gcgctgtaca
      121 ccgggaccgc catacacctc gatgagcacg ggataacgct tgcccgcctc gagggtgcct
      181 ggcttcagca atttgtagtg catcgcctgg ccgtcggccg ccgcgaaggt gccgaactcc
      241 gggcgcacat gttcatcgag aaagcgccca tacggatgcg cggcatcgag ctggttgggc
      301 aacattgccg acaaccggcg gccacccgag gtccgcagcg tgacgttgcg tggaagattc
      361 gcttcggaga aggtatcgac gaacacacgc gcatcctgcg acatggcgat gctgtgccat
      421 ccggcgcccg cggtgactcg ttgcgcgccc gcgggctcat cgagcgaaac ccagaacaac
      481 tgccgctcga cgggcgaggg ctgattggcg gtgaaataca cgcgccgcgc gcgttcgtcg
      541 acggaacgaa tcgctgcctc tggtggttcg cccaccacca tgaactcgcc cgaggtgagc
      601 tgccggatca ggttgccgcc gttgccatac aaatacaggt gctgatagcc gctgcgcgac
      661 gatgcccaga tgaactgcgg cgagtgctgc aggaaagtga gttcgcgatg cagcggcacc
      721 cagtgttcgc tgcgttcggt cagcagcacg cgaccgcgtc ccgagagcgc gtcgaaacgc
      781 atcaactcca gggtcttctg gtcacggctc tggcgttgca cggcgatgcc ccggctgtcc
      841 gggaaccagt ccacgcgagg tagatagatg tcggtttccg cgccgaggtc gagttgcagg
      901 cggctctcgg ccgccaggtc cgcgacgaac aggtccacgc gggcattgcg cgccccggtg
      961 gccgggtatc gctggcgcat cacgcgggcg cccgacgcct ggatttcgaa gcgttccacc
     1021 tcggcaacct gcgactcgtc gacgcgcgcg agcgcgatat gccgctcgtc gggcgaccac
     1081 cagtagccgg tgtcacgatc catctcttcc tgcgcgatga attcggccat gccgaagctc
     1141 actgcgccgc cacctttact cgtcaccgcg cgctccttgc cggtggcgag atcgatgacg
     1201 tggagattct gatcgcgaac gaagctcaca taactcgagt gcggggagaa ccgcgcatcg
     1261 gtttcgaagg catccgtatt cgtgagccgg cgcacggcat cggcaggttt gcggccgagg
     1321 tcgtacacgt acaggtcgcc attgagggga atcagcaggt gacggctgtc gggcgcgaag
     1381 ctgtattcca cgatccctga gtacgcagag gtccgctgcc gttcgcggcg cgattcttct
     1441 tcagccgaga gcgcgcggtc cgcgcccgcc agcgtgcgcg aatccacgag cagccggtcg
     1501 cgcgacaggc gcacgtcgaa cgcccagaga tcgaaacgat ccttgtcgtc ttcgcgtgcc
     1561 ttcaggtagg ccacgagcct gccgtccggc gagatctcca cgcctcgcag ggttggcccc
     1621 gacagatccg gcgcggcaaa cagtcgctcg agcgtgagct tctccgctcg cgcgggcgcg
     1681 gcgagagtag tggcgaagag cagcgacaga gccacgaggc ttgcgccgaa acgacgataa
     1741 atcagggcgc gccggacagg cgcgcgcaca ccgtttgata cactttggtt caatttcatt
     1801 tggggagttt agggatgctc aacaatcctc gccacctggg cgcgctcgtc gcgaccacgc
     1861 tggccgcact cattgccgca tgcgcgatgt caccgaaggc gcagcacacc cctgttcctt
     1921 cacccgctgc catttcttcg ttccagtatc cggcggccgt gcgcggcgaa gtcgtcgaca
     1981 cctacaacgg cgtcagcgtt cccgatccct accgctggtt cgaagacgcg atggcaccgg
     2041 caactcgcga ttgggtgacc gccgagaacg cgctgtcgca gccctacctc gaaaaacttc
     2101 cgcagcgtgc gtggctcggc gaacgcctca aacaactctg gacctacgaa cgcttcggtg
     2161 ttccgcagcg cgaaggcgac aaatacttct atatgcgcaa tgacggcacg caggaccaga
     2221 gcgttttgta cgtggccgac gcgctcaacg ccacggcgcg cgtgctcgtc gatcccaacg
     2281 gcaagcgcga cgatgccacc atcgcgctgt cccaatgggt gccgagcccc gacggtaatc
     2341 tgctcgcgta tgcgctttcc gatggcggca ccgactggaa tacctggcac ttccgccgcg
     2401 tcgccgacgg cacggatttg ccggtgattc tcaagttcag caagttctac gcggtctcgt
     2461 gggcgcgtga cagctcgggc gtctactaca gccgttatcc gctcaagccg ggccagaagg
     2521 ccgaggaagc cgagcgtggc gatgacgccg gccgtccgga cgtgtacttc cacaagctgg
     2581 tcgaagcgca atcggccgat cgcctggtgt accaggtcac cgatcacccg acacgcgtgc
     2641 cctcgggcca ggtcaccgaa gacgggcgct atctcgtcat cggcttgttc gacggctacg
     2701 aatcgaacgc catcctcgtg caggacctgc gcaagccgga tgcgaagccg cagcccgtgt
     2761 tcacggcctg ggacgcgctc tacaacttca tcggcgccaa gggtgacgag ctctatttcc
     2821 agaccaccaa caatgcgcca cgcggacgcg tgatcgcggt caacgcgaac gaccccgcgc
     2881 ccgcgcgctg gcgcaccatc gtgccgcatg gcgacatcgc gatggccaac gtcagctaca
     2941 tcggcggccg tgtgatcgtc gaatattccc gcgatgcgcg cagcgtcgtg cggctgttcg
     3001 aagtgagtgg cgcgcaagcc ggcgaagtga aactgcccgg gctcggcacc gccacggcct
     3061 tccagggcac gggcgcgaac ccggaagcgt tcttctccta ttcggactat ctatcgccga
     3121 cgcgcatcct gcgcctcgac gtcgcgtcga acaccgtcag cgagttccgt acgccgaaag
     3181 tgcctgcgga tttttcacca tacatgaccg agcaggtgtt ctatcccagc aaggacggta
     3241 cgcgcgtgcc catgttcatc acgcgccgca aggacgcgcc gcgcgatggc aaccgtcccg
     3301 tgatgttgta cgggtacggc ggcttcaacg tcacactgtc gccgtcgttc agtccgtcca
     3361 tccagtcgtg gctggagatg ggtggcgtct acgcggtggc caacctgcgc ggcggcggcg
     3421 aatacggcga ggaatggcac caggccggta ccaggctcaa gaagcaaaac gtgttcgacg
     3481 acttcatcgc cgccggtgaa tacctgatcc gcgagaagta caccaatccg aagcgcctcg
     3541 cgattctcgg ccgcagcaac ggcggattgc tggtcggtgc cgcgctcacg caacggcctg
     3601 atctgttcgg cgccgcattg ccgggtgttg gtgtcatgga catgctgcgt tatcacaccg
     3661 ccagcgccaa cgcgcgccag tggtcgagtg acttcggcat ctccgaagac tctgcggagt
     3721 tcaaggcgct gcacgcgtac tcacccgtgc acaacatcaa gacgggcacc tgttatccgc
     3781 ccacgctcgt gaccacggcc gatcgcgacg atcgcgtggt gccgtggcac agctacaagt
     3841 tcgccgccga gatgcagcgc gtgcaggctt gcgcgaatcc cgtgctgatc cgtatcgaga
     3901 cgcgcgctgg ccacggtgcc ggcaagccgg tgtggatgca gatcgaagat atcgccgatc
     3961 agttcggatt cgtggcgaat gcgctgggga tgccggcgcc cggcggctag aacgtcttac
     4021 ctgtctggaa agtcttgatt atctggacgt acgccgccgc ttcggcggcg ttttccgtcg
     4081 tggcgcgcag cgcctgacta agtacagcag gtgagcgtga acggcacatc gtcggtagtt
     4141 tgaaccggcc gcgtctgcag gccgttccac gcgagcagcc gcaccgagca gcggtagtga
     4201 gagccgctgg tttccggata caggccggcg tccgccggca gtgcgatgcg cacgagctgg
     4261 atcggcgtgt cacgctcgaa ggtgatgtga taaacgccgc cgcgcgcgat ttcctggcgc
     4321 ggcttgccgt gctggcgcgt gatccacagc agctcggcga tggcgtcgca cagcggccgc
     4381 agcctggcta accagtcgtt gaacgagcgg gcacgcgcgt cggtggcctg gttgagccag
     4441 aagaagaaat ccggcagatc gaattcgcag gtaccgccgg ggatggcgct gcgatgtttg
     4501 atggcggaga ggaactccga atccttgagg ccggcgagat agttagagcc gcaggccacg
     4561 aggtcggcac gcagccgcag caggttgctc atgacggtgc gcaggcgggc ggagtcgact
     4621 cccggcttgc tctggaacac gttgagctgc gcgagatgac gctcgagttc cttctgcacg
     4681 tcggcgcgca cgtcaccgcg cgcggcgatg gcgaggatgt cgagcaggct gcccatggcg
     4741 gcgcggctgc cccactggct ctgcatttcg ttgtggtaca gcgcctggct gtagagaaaa
     4801 tcgagacgca ggaaggtgcg catgcgctcg ttgagcggct gctcgaatat cagcggcgcg
     4861 accgcagccg cggccgcgct ctcgtgctgt tgatcgctcg ttttctgggc ttcctcggac
     4921 atcgggctat tctgcgcgac cgcggcgccc tccgtaaaca cgattggcgg cagctcgttg
     4981 tcttcggcgc tcatgagccg gccttgtctg attgcgcttt ggctgcggct acatactggc
     5041 ggtgcagctt ttcgacctga tcgcgcaatt gcgcgatgtc gccatcgttg tggatgacgt
     5101 cgtctgccgc ggcgagccgc gccgcgcgcg gcgcctgggc ggcgagcatg cggcgagctt
     5161 cttcatgcgt tgatccgtcg cgcgccgtga gccgcgcgag ctgcagctcg ggctcgcagt
     5221 cgaccaccag gacccgatcg aagcgcgctt cgccgccggt ttcgaccagg agagggatcg
     5281 cgacgatcac atacggcccg gtcgccgctt cgcaacgcac atcggtaagt tcgcggatgg
     5341 cgggatgcag caacgcctcg agccactttc gctgcgccgg gtcctcgaaa atccgtttgc
     5401 gcagctcctg gcgatcgaga gatccgtcgg cgccctgtac ctgcatgccg aaatggtcgg
     5461 tgatcttgcg tagcagcggt gatccgggcg ccaccacctc tcgcgccagc agatcggtgt
     5521 cgacgatcgg cacaccgagc gcggcaaaga ggtttgcgac ggtgctcttg ccggaggcga
     5581 tgccgcccgt gagcccgatg cgaaacccgc cgtgcatcgt tgcgctcagg gcggagacgc
     5641 gtacagcgcg agatatcggt cgacagtctg gtggccgacg agcaggaaca gccagcccgc
     5701 ggccgccagg aaggggccga aggcaatcgg cgtgtccatg cccttgcgct ggcgataaat
     5761 gatgtagacg ccggccacgc aacccacgag cgaagcaaac aggatgatgg gcagcagcat
     5821 gcgccagccg aaccaggcgc cgaaggcagc gaacagcttg aagtcgccgt atcccatgcc
     5881 ttccttcttc gtgatgagct tgaagatcca gtagacggtc cagagcgaca ggtagccgca
     5941 catggctccc acgatgctgt ccgctggcgt gactggtgcg gcaccctggg accataccgg
     6001 gtgggtggtg ctgaggagga gaccgaccca cagcagcggg taggtgaggt catccggcag
     6061 caactgtgtg tcgaagtcga tcatcgtcag ggcgatgaga gaccaggtga agaacagtcc
     6121 ggcgagcgcc agggtgccat aaccaaatcg ccaggcgatg agcccggacg ccaaaccggt
     6181 gaagagctct attaacggat aacgcgcgct gatcggcgtc ttgcagttgg cgcatttacg
     6241 ccccagcgcg agccagctca ccaccgggat gttttgcaga gcggtgatgg gcgccttgca
     6301 cttcggacag gccgagcgcg gcgtgacgag attgaaggcg ggcggcggcg gagcgtcggg
     6361 attggccagc gactcgcatt cggcacgcaa cgcgttgtcc atcatcaccg ggacgcgata
     6421 gatcacgacg ttgaggaagc tgcccaccag caatcccagc aggacggtgc aggtgatgaa
     6481 cagcgcgggg ctgtcggcga gcggtgcgag tagttccatg gttcgatctg ggccttcgtt
     6541 aaaaagaaac gccgccccca tctttcagaa atgggcgcgg cgtttcatcg actcgatgcg
     6601 gggtcggtgt gcctttacac cacggcgccc agtttgaaga tgggcaggta catggcgatg
     6661 accatgccac cgacgagcac gcccagcacg gccatgatca gcggttcgag cagcgccgac
     6721 atgctgtcca cggcattgtc cacttcggct tcgtagaacg tggcgacctt gcccgacatg
     6781 gcgtcgagcg cgccggcttc ttcaccgacc gcgatcatct ggatgaccat gttcgggaac
     6841 agtccggtgt tttccatgga ccgctgcaga cgctgacccg tggcgacttc gtcgcgcatc
     6901 ttcagcaccg cgttttcgaa cacgatgttg ccgcaggcgc cgga
//
LOCUS       DASQJS010000180         3371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_102262, whole genome shotgun sequence.
ACCESSION   DASQJS010000180 DASQJS010000000
VERSION     DASQJS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102262"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VEW08_07525"
     CDS             <1..791
                     /locus_tag="VEW08_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ40622.1"
                     /translation="RLQADTRPVVQILVDGSQPQLAVVAGGIAAVPLLTRHGEGPYSG
                     GKRMAPRRIEIRTLYNPEKRTAVQIVPALIGVILTMTMVLFTSGAIVRERERGNLELL
                     IATPLSSLELMVGKLLPYVVIGLIQTTIILIVGAWLFSVPVVGRLVDLYAAALVFIAA
                     TLGLGLFVSTLAQTQFQAFQLAFLTLLPSILLSGFMFPFEGMPVAAQWLAELLPLTHF
                     NVIIRGIMLRGADLPEAWLQLVKLAAFLVVMLMVAVVRFKKRLD"
     gene            852..1682
                     /locus_tag="VEW08_07530"
     CDS             852..1682
                     /locus_tag="VEW08_07530"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HYJ40623.1"
                     /translation="MDEPTQEERAHRFRALHTSGTFVIANCWDGGSARILTSLGFSAL
                     ATSSGASAGTLGRLDGNVSIDEAMAQARIICGATSLPVSADLEKGFGDDPAATAKAVQ
                     LAARAGLVGGSIEDASGHGNAPIFDIGLATERVAAAVDAARKLPFPFMLTARAENYLH
                     GRPDLDDTIKRLQAFESAGADVLFAPGLPDLAAVRAVCSAVSKPVNFMVGIRGRSFSV
                     TELGQAGVRRISLATSLYRAAMTGLTEAAREIRDSGTFHYLDDTMSSGEFAKYIQGGS
                     "
     gene            1679..2200
                     /locus_tag="VEW08_07535"
     CDS             1679..2200
                     /locus_tag="VEW08_07535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ40624.1"
                     /translation="MKLIDCEPGRHSAPILAIFNEAIANSTALYDYRPRTADSMAGWF
                     EAKARGRYPVIGIEDEAGTLVGFGSYGPFRAWPAYKYTVEHSIYIDTRFRGQGHGRVL
                     LEAIIAAAVKQDYHVMVGGIDASNAVSIRLHESLGFTHCGTVQQAGFKFGRWLDLAFY
                     QRILATPVAPVDG"
     gene            2291..>3371
                     /locus_tag="VEW08_07540"
     CDS             2291..>3371
                     /locus_tag="VEW08_07540"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HYJ40625.1"
                     /translation="MKLLALGALLATLASSAIAQTPVVPSPNGMGDADKEDWIVLFNG
                     KDLNGWTPKLAKHELGDNFGNTFRVEDGLLKVRYDKYKSFDGQFGHLFYKDPYSYYRL
                     VVEYRFVGDQAPGNPGAWAFRNSGAMLHSPDPHTMPRDQTFPISIEAQFLGGKSDGNP
                     RSTANMCSPGTEVVYRGTIYKDHCLNSTSPTFDGEQWVRAEMVVHGSGTITHYANGQK
                     VLEYSMPQYGGGVVDNYDATTKLDGKLVDGGYISLQSESHPIDFRKVELLNLAGCTDK
                     AATNYKSYYVKPVPEACRFAEGVKPAMRRPGEYPVSAEALPQDGIPKGRLEGPFEFHS
                     KIIANTVRRYWIFVPAQYNDKKPKPA"
BASE COUNT          634 a         1138 c          990 g          609 t
ORIGIN      
        1 gccgcctgca agccgacacg cgcccggtgg tgcagatcct ggtcgatgga tcgcaaccgc
       61 aactggctgt cgtcgccggc ggcatcgcgg cggtgccgct gctcacccgc cacggcgaag
      121 gcccatattc gggcggcaag cgcatggcgc cgcggcgcat cgaaatccgc acgctctaca
      181 acccggagaa gcgcaccgcg gtgcagatcg tgccggccct catcggcgtg atcctgacga
      241 tgaccatggt gctgttcacc tcgggcgcca tcgttcgcga acgcgagcgt ggcaacctgg
      301 aattgctgat cgccacaccg ctcagcagcc tcgagctcat ggtgggcaaa ctccttccct
      361 atgtcgtcat cggcctgatc cagacgacga tcatcctgat cgtcggcgcc tggctgttca
      421 gcgtgccggt cgtgggaagg ctcgtcgatc tgtatgccgc ggcgctggtg ttcatcgcgg
      481 ccacgctggg cctgggactc ttcgtctcca cgctggcgca aacgcagttc caggctttcc
      541 agctcgcttt cctcacactg ctgccttcga tcctgctgtc cgggttcatg ttccctttcg
      601 aaggcatgcc cgtggcggcg cagtggctcg ccgagctact gccactcacc catttcaacg
      661 tgatcatccg cggcatcatg ctgcgcggcg cggatctgcc cgaagcctgg ctgcagctcg
      721 tcaagctcgc ggcatttctc gtggtgatgc tcatggtcgc agtcgttcga ttcaagaagc
      781 gcctcgacta aaaagtggtg ccaggcaccg tttcctagga atcggtgcct gacccctttt
      841 agactagtgg catggacgaa ccaactcagg aagaacgggc acaccgcttt cgcgcgctgc
      901 acaccagcgg caccttcgtc atcgccaact gctgggacgg cggctcggcg cgcatcctca
      961 cctcgctcgg cttttcggcg ttagcgacat ccagcggagc ctcggccggc acgttaggcc
     1021 gtctcgacgg caacgtctcc atcgatgaag cgatggcgca ggcgcggatc atctgcggcg
     1081 ccacgtcgct gcccgtgtcc gcggatctcg agaagggttt tggcgatgac ccggccgcca
     1141 cggcaaaagc cgttcagctc gcggcccgtg ccgggctcgt gggcggctcc atcgaagatg
     1201 cctcgggcca tggcaacgca cctatcttcg atatcggtct cgccaccgaa cgcgtcgcgg
     1261 ccgccgtgga cgccgcccgc aaactgccgt ttccgttcat gctcacggcg cgcgccgaga
     1321 actatctaca cggccggcca gacctcgacg acacgatcaa acgcctgcag gcgttcgaaa
     1381 gtgccggcgc cgatgtgctc ttcgcccccg gccttccgga tctcgcggcc gtgcgcgcgg
     1441 tgtgttccgc ggtcagcaaa cccgtgaact tcatggtggg cattcgtggc cggtcgttct
     1501 cggtgacgga actcgggcag gcgggcgttc gccgcatcag cctcgccact tcgctgtatc
     1561 gcgccgccat gaccgggctc accgaggccg cgcgcgagat ccgcgactcc ggcacgttcc
     1621 actacctgga cgacaccatg tcgagcggcg aattcgcgaa atacatccag ggcggctcgt
     1681 gaaactcatc gactgcgagc ccggacgtca ttccgcgccc atcctcgcaa tcttcaacga
     1741 agcgatcgca aactccaccg cgctgtacga ctacaggccg cgaaccgctg attccatggc
     1801 gggctggttc gaagccaagg ccagaggccg ttatcccgtc atcggcatcg aggacgaggc
     1861 cggcacactc gtgggcttcg gcagctacgg cccgtttcgc gcctggcccg cctacaagta
     1921 cacggtcgaa cactctatat atatagacac gcgcttccgc ggccagggtc acgggcgagt
     1981 actgctcgaa gccatcattg cggccgcggt caaacaggac taccacgtca tggtcggcgg
     2041 catcgatgcg tcgaatgccg tgagcatccg gctgcacgaa agcctcgggt tcactcactg
     2101 cggcaccgtg caacaggcgg gattcaagtt cgggaggtgg ctcgatctcg cgttctatca
     2161 gcgcatactc gcgacgccag tcgcgccggt ggacggataa gaaatcaact ccggggcact
     2221 tttgagattc atcgtgttgt cagccgtgat cgccacagca ccccttcgcc agacaggaat
     2281 aaacatggcc ttgaaactac tcgcactcgg cgcgctgctc gccactcttg cttcctccgc
     2341 catcgcgcaa acaccggtag tcccttctcc caatggaatg ggggacgccg acaaggaaga
     2401 ctggatcgtc cttttcaatg gcaaggacct gaatggctgg actccgaagc tcgccaaaca
     2461 cgaactcggc gacaacttcg gtaacacctt ccgcgtcgaa gacggcctgc tcaaggtccg
     2521 ctacgacaag tacaagagtt tcgacgggca gttcggacac ctcttctaca aggaccccta
     2581 ttcgtattac cggctcgtgg tcgagtaccg tttcgtcggc gaccaggcgc ccggaaatcc
     2641 tggcgcctgg gcgttccgca acagtggcgc catgctgcac tcccccgatc cgcacaccat
     2701 gccacgcgac cagaccttcc ccatttccat cgaagcgcag ttccttggcg gcaagagcga
     2761 cggcaatccg cggtccacgg ccaacatgtg cagcccgggc accgaagtgg tgtaccgggg
     2821 gactatctac aaggaccatt gcctgaactc gacctcgccc actttcgacg gcgaacaatg
     2881 ggtgcgcgcc gagatggtgg tacacggatc cggcaccatc acgcactacg ccaatggcca
     2941 gaaagtgctc gagtactcca tgccgcagta cggcggcggc gtggtcgaca attacgacgc
     3001 cactaccaaa ctcgatggca agctcgtcga cggcggctac atctcactgc agagcgagtc
     3061 gcatcccatc gacttccgca aggtagagct gctgaatctt gccggttgca ccgacaaggc
     3121 agccaccaac tacaagagct actacgtcaa acccgtgccg gaggcctgcc ggttcgccga
     3181 aggcgtgaag cccgcgatgc gccggcctgg agagtacccg gtgagcgccg aggcgctgcc
     3241 gcaggacggg attccgaagg gccgtctcga gggaccgttc gagttccata gcaagatcat
     3301 cgccaacacg gtgcggcgtt actggatttt cgtgcccgcg cagtacaacg acaaaaaacc
     3361 caagcccgcg a
//
LOCUS       DASQJS010000181         3481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1671227, whole genome shotgun sequence.
ACCESSION   DASQJS010000181 DASQJS010000000
VERSION     DASQJS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671227"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VEW08_07545"
     CDS             complement(<1..1101)
                     /locus_tag="VEW08_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40626.1"
                     /translation="MAFCTTAAAQAASPADLLAQLETTVANEDEKAAQELWKRLTPEF
                     DHLSRNDQGRFLVVQGLIQEDILRDINSADQSFNRVITLLDASPEPTQALADAYYERA
                     YIKYIRTNNTADYCPDREKAVALTRKLNTRSKLPKYLTALSFCYSDSPARLQQGLAVL
                     NEALTLAEAMQLPPQERGLIYNATAILYRKNQLYSQAYEYSGLAYAQWKSIGALPSMD
                     TQQHNLLINAIGMGDLDKAELHGEELFALADAAPQYKDFRFFANLDSGLVAFARNDLP
                     RAIRLFEEARREERNTEETAFIAGNRAQLAAAYFLHGDLNAALREAAAVTQLPGYPSL
                     DPDQKQWVQALQQYSSKRPVQAMQGLFLLYRAQ"
     gene            1303..1887
                     /locus_tag="VEW08_07550"
     CDS             1303..1887
                     /locus_tag="VEW08_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40627.1"
                     /translation="MGNEDLLPVLNAKPGQRPLAARSDRDLTTEFERALAAGDGITGA
                     HCIHERWMRGDFAAHIETALDLLWKRAGASIPDWLPMRYVSWLPTAYEVAARFHANSG
                     RSNIYLILLDYSDRRGDDHGVYVGMSRYSPSQRFDQHKAGIRAAGSVLKRGIEVLTGP
                     TLHLQHIRRGEAARIESALAAALGDAGIHVEGGH"
     gene            2058..2840
                     /locus_tag="VEW08_07555"
     CDS             2058..2840
                     /locus_tag="VEW08_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40628.1"
                     /translation="MSRNCFCHRTLGLVVLVTLLQACGDPKSSSTSGPDGGPVPTSKM
                     TIAMKVESGDSGIAVVRATLNDGSALGDSYRLDGGDFFRACVNGVCRTMADNDSLDSP
                     DYIARFDYQPGVDFVVSFNRQEAQNAPDSRAALPQPFTIVTPANHQQVTDGETVLVSW
                     TPTGAPARVSLRYAVNCTLLSGNVVTSGELGTDSNGDGREAVRINPIVDAARSGTTST
                     ITRCSIDVTVSHELPGRIDPAFRNGTAVGIVSRKVNLDYVPR"
     gene            complement(2837..>3481)
                     /locus_tag="VEW08_07560"
     CDS             complement(2837..>3481)
                     /locus_tag="VEW08_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40629.1"
                     /translation="YETSGVHGALQFLEQRAADYPINLLTVPCVSPWAYERVQRWNAD
                     AVDPNRSFKEGTTAAESAALLRLVAPLRGRFLVHVDLHETTDSDESEFRPALAARDGK
                     PFEPGLIPDGFYVVDDSENPQRQFQRAIIEAVARITHIAPADHKDEIIGSPVISPGVI
                     DYPLQKLGLCAGITGARYTTTTEVYPDSPRATPEQCNAAQVAACCAAIEFALRR"
BASE COUNT          643 a         1064 c         1105 g          669 t
ORIGIN      
        1 ttgcgcgcga tatagcaaaa acaaaccctg catggcttgt acggggcgct tgctgctgta
       61 ttgctgtaag gcctgcaccc attgcttctg gtccggatcg agggacggat acccgggtag
      121 ttgggtcacg gccgccgcct cgcgcagcgc agcgttcagg tcaccgtgca gaaagtaggc
      181 ggcggccagc tgtgcgcggt tgccggcgat gaatgccgtt tcttcggtat tccgctcctc
      241 acggcgagcc tcttcgaaca gacgaatggc gcgtggcaga tcgtttcgcg cgaaagcgac
      301 caacccggaa tcaagattgg caaagaaacg aaaatccttg tactggggcg cggcgtccgc
      361 gagggcaaac aactcctctc catggagttc cgccttgtcg agatctccca tgccgatcgc
      421 gttgatcagc aggttgtgct gctgggtgtc catgctgggc agggcgccga tggatttcca
      481 ttgggcgtag gcgagcccgg aatactcgta cgcctggctg tacaactggt tcttgcgata
      541 gaggatggcc gtggcgttgt agatcaatcc gcgttcctgg ggaggcagct gcatggcttc
      601 cgcgagcgtc aacgcctcgt tcaggaccgc caagccctgc tggaggcgcg ccggtgaatc
      661 cgaatagcag aaggacaacg ccgtcaggta cttgggcagt ttgctgcgcg tgttcagctt
      721 gcgcgtcagc gcgaccgcct tctcacggtc gggacagtaa tccgccgtgt tgttggtgcg
      781 gatgtacttg atataggcgc gctcgtagta cgcatcggcc agggcttgcg tgggttccgg
      841 ggaagcatcc agcagtgtga tgacgcgatt gaaggattgg tccgcgctgt tgatgtcgcg
      901 gaggatgtct tcctggatga gaccctgcac caccaggaag cgcccctgat cgttcctgga
      961 cagatgatcg aactctggag tcagccgctt ccaaagttcc tgcgctgcct tctcatcttc
     1021 attggcaacg gttgtctcca gctgcgccag caggtcggcg ggtgatgcgg cttgtgccgc
     1081 ggccgttgta cagaaggcca tgggccacaa ggccgtcagc aagaaaacga gaacgtggtg
     1141 cgagaaatcc ctgggctgca agatccgact ccctttcggc tcatcgcgcg tcgtctaaca
     1201 tggagttcgc gcgcgcgcac tggcgcgtgg gcgttgatgg tagcaaacgg attcgaatgg
     1261 agacgtgaac taacgcaatc tacagagcta tagaatcatt gcgtgggaaa cgaagacttg
     1321 ttacctgtgt tgaacgcaaa gccgggccaa cgaccgctgg ccgcgcgatc ggaccgcgac
     1381 ctcacaacgg agttcgaacg cgcgcttgcg gcgggcgacg gcatcaccgg cgcgcactgc
     1441 atccacgaac gctggatgcg cggcgacttc gccgcgcaca tcgaaacggc cctcgatctc
     1501 ctgtggaagc gtgcgggcgc atcaatcccc gactggttgc ccatgcgata cgtcagttgg
     1561 ttgcccaccg cgtacgaagt cgccgcgcgt tttcatgcca acagcggccg ttcgaacatc
     1621 tatctcatcc tgctcgacta ttccgatcgg cgcggcgatg accacggcgt gtacgtcggc
     1681 atgagtcgtt attcaccctc ccaacgtttc gaccagcaca aggccgggat tcgcgccgcc
     1741 gggagtgtgt tgaaacgtgg tatcgaagtg ctgacggggc cgacgcttca cctccagcac
     1801 atcaggcgcg gcgaggcagc gcgcatcgaa tcggcgttgg ccgcggcgct cggggatgcc
     1861 ggcatccacg tcgagggagg tcactgaggt tcccacgact cctgggcaat cgcgcagctg
     1921 gccggtagca catttcgccg ccgacagtat cggcttccgc tactaacttc cgggcaattg
     1981 tttcacaata caaccggagt aaaagttcac cggcaaacaa acaaacaaac gcctagccac
     2041 ttcagggaga gcgggccatg tcacggaact gcttttgtca tcgaacgctt gggctggttg
     2101 tcctggtcac actgctgcaa gcgtgcggcg atccaaagag cagttccacc agcgggcccg
     2161 atggcggacc ggtgccgacc agcaagatga ccatcgccat gaaagtggaa tccggggact
     2221 cgggaatcgc ggtggtgcgc gcgaccctga acgacggcag cgctctcgga gattcgtacc
     2281 ggctggatgg cggcgacttt ttccgcgcgt gcgtcaacgg ggtgtgccgc accatggccg
     2341 acaacgattc gctggattcg ccggattaca tcgcccgctt cgactaccag cctggagttg
     2401 acttcgtggt ctcgttcaat cgccaggagg cgcagaacgc tccggactcg cgcgctgccc
     2461 tgccgcagcc tttcaccatc gtgacgcccg ccaaccacca gcaagtcacc gatggcgaaa
     2521 ccgtgctggt ctcgtggacg ccaaccggcg cgcccgcgcg tgtgtcgctg cgctacgccg
     2581 tgaactgcac ccttctttcc ggaaatgtcg ttacgagcgg tgagctcggc accgactcga
     2641 acggcgatgg ccgcgaagcc gtccgcatca atcccatcgt ggacgctgcg cgatcgggca
     2701 caacgtcgac gatcacccgc tgcagcatcg atgtgaccgt gagccatgaa ctgccgggtc
     2761 gaatcgatcc ggctttccgc aacggcaccg cggtgggcat cgtgtcgcgc aaggtcaatc
     2821 tggactacgt cccgcgctag cggcgcaacg cgaactcgat cgccgcacag cacgcggcga
     2881 cctgggccgc attgcactgc tcgggtgtgg cgcgcggact gtcgggatac acctcggttg
     2941 tcgtcgtgta tcgcgcgccc gtgatgcccg cgcacagtcc gagtttttgc agcggatagt
     3001 cgatcacgcc cggcgaaatg actggcgagc cgatgatctc gtccttgtga tccgccggtg
     3061 cgatgtgcgt gatcctggcg acggcctcga tgattgcgcg ctggaattgg cgttgtggat
     3121 tctcgctgtc gtcgaccaca tagaacccgt cagggatgag gcccggctcg aacggcttgc
     3181 cgtcgcgcgc ggctagcgcg gggcggaatt cgctttcatc ggagtcggtc gtctcgtgga
     3241 ggtccacgtg caccaggaag cgtccgcgca gcggcgcaac caggcgcagc aacgccgccg
     3301 actcggcagc ggtggtgcct tccttgaacg accgattcgg atcgacggcg tccgcgttcc
     3361 agcgttgaac gcgctcgtag gcccacggac taacgcaggg caccgtcagg agatttatcg
     3421 gatagtccgc tgcgcgttgc tcgaggaact gcagcgcgcc atgcacgccg ctcgtctcgt
     3481 a
//
LOCUS       DASQJS010000182         6395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4498427, whole genome shotgun sequence.
ACCESSION   DASQJS010000182 DASQJS010000000
VERSION     DASQJS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4498427"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..584)
                     /locus_tag="VEW08_07565"
     CDS             complement(162..584)
                     /locus_tag="VEW08_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HYJ40630.1"
                     /translation="MTKRSWIIMAAGLVLGASLQMTLATGATPAGKVFEIRTYHTFPG
                     RLDALHKRFREHTMKLFEKHGMTNVGYWVPQDSPARETTLIYVISHASREAAKANWAA
                     FIADPEWKKISEASQVDGKIVERIESVFMDATDYSPIR"
     gene            662..2110
                     /gene="pyk"
                     /locus_tag="VEW08_07570"
     CDS             662..2110
                     /gene="pyk"
                     /locus_tag="VEW08_07570"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HYJ40631.1"
                     /translation="MPERQMHSRTKIVATLGPSTDSIEVLTEMVRAGLDVARVNFSHG
                     KHEEHGRRIDMVHEAARRAGRYVGILADLGGPKIRIESFTNKKVDLKDGQAFALDTAH
                     DINAGNEQVVGCAYKDLPKDVKAGNILLLNDGLIELEVTQVSGTRIDTKVIAGGELSN
                     RKGVNLKGGGISAPALTDKDREDIKFACNRGVDYVAVSFPRDAADMNLARDLVRAAGG
                     HAHLCAKVERHEAIHNLTEIIEASDVIMVARGDLGVEMGYAAITGLQKTIIHESRTRN
                     KVVITATQMMESMITSPVPTRAEVSDVANAVMDGTDAVMLSAESAVGKHPIKAIQAME
                     EVIAGAEKYEITHNRMRHRAEGIVDRSEEAIAMAVMYTSNHMKVRAIVALTESGTTPL
                     WMSRARSDIPIYAFTRHESTRRRVTLYRGVFPVPFDIPVVRTNELFKAILTMLLQLEL
                     VEKGELVIVTKGELTGITGGTNAMTILRVPKD"
     gene            2119..4500
                     /locus_tag="VEW08_07575"
     CDS             2119..4500
                     /locus_tag="VEW08_07575"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HYJ40632.1"
                     /translation="MKWLRWLRWTLGFIVVCVALAVGGAWWAVRQSLPQIEGTANAPA
                     LGKEATIDRDARGIPVITATTRADLAYALGYAHGQDRFFQMDLSRRLAAGELSEMFGA
                     VALKQDRRTRRFGFRAVARRVIEATPAEERGIVEAYAHGVNAGVASLHARPWEYLLLR
                     VQPREWLPEDSVLVVHSMWWQLQYGTLRDEVARRRLERAAAARSDARAAHELIAFVYA
                     GHSDWDSPNYAADSPCVQAACTDSAGVLTLSFPGQMRFTGAADEPDSEPTAPGSNNWA
                     VAGIHTRSGVALIANDMHLDLGVPAVWYPARLRVTGNPAVDITGVTLPGTPAVAAGSN
                     GQVAWGFTNSYGDFADARWGKCVSADYGVRREHIAVKGGDAQEVTYREVGEGVVLDGE
                     EYADDEKGGECLQAAWLATRPEATNFGLLRLEGARNIDDVLAVAPLVGIPGQNLVVGD
                     TRGRIAWTLLGRVPANHGPDRLFGPIEFLDGVDHPRITDPPVGRLWTANQRVVSGPLE
                     TVMGDDEVDVGAGGYDIGARARQIRDDLLSLPHPATEADMLAIQLDARALFAARWRDL
                     LLALIDEDAMHDAPLRREFRELVSKGATEASPEAVGYRLVRTYRSNTLNAMWRSLVTG
                     LLGEKAVVRRAAQFEAAGWRLVNERPSAVSPPGGGDWRAFLLRRLDATIAELLKDCAS
                     LETCEYGQMEPVEVSHPLSGALPLLPRLLDMPTRALAGDQHMPRVQNGSFGASERFAV
                     SPGRERDGYLQLPGGPSGNPLSPFYRSGFEDWANGVPTPFLPGSVAYRLTLRP"
     gene            4582..4986
                     /locus_tag="VEW08_07580"
     CDS             4582..4986
                     /locus_tag="VEW08_07580"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HYJ40633.1"
                     /translation="MPHNVSDLGDLEREVLQLVWGHGPSSADWVQKALSRPLKESTVR
                     TVLKRLEAKGHLTHRVDQRTFIYEAADTRGRAAARAVKRIVDRFCSGSLEEVLVGLVD
                     TRILDRRELQRLAEKIARAQSDSDGQPKRGKK"
     gene            4983..6203
                     /locus_tag="VEW08_07585"
     CDS             4983..6203
                     /locus_tag="VEW08_07585"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HYJ40634.1"
                     /translation="MTPEILLSSALRLLLLGAAVWLALRVIPVRNPHVEVLAWRMILI
                     AGLALPALLYWRLAPSFATSLELPVIAAAGVGGTNAPPAMASTSRLTAGVLVTIYLII
                     ALLLLVRLLSGLAAMWRVSRAARPMTTPDDVRMSALVRSPATFGTIILLPADAHTWPA
                     GRLDAVLAHERAHVSSRDGFWSWLAQLHAAIFWFSPIAWWLRRRLEALAETTSDDAVV
                     AARHDPVAYAALLLDFARHPNSRRVAMSVAESNVPMRIERLLARTPPATALPRVARWS
                     AFAALIPIAVFAASTTRATPPAEPAASAAAAVPTPAPIHVVGVRLIRPANPDIFYPAI
                     AKHEKVSGYAIVEVDVDVLGQLVDARVLKVQPTDPQFGFADAALQVARNTTYGNTTQQ
                     VGSMKFMVKFELAH"
     gene            6267..>6395
                     /locus_tag="VEW08_07590"
     CDS             6267..>6395
                     /locus_tag="VEW08_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004879284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ40635.1"
                     /translation="MTETRFTGTERYVATPDLKMAVNAAVTLGRPLLIKGEPGTGKT"
BASE COUNT         1112 a         2123 c         2063 g         1097 t
ORIGIN      
        1 tgagcgccat cgatcagctg acgatcgact cctcgaagtg aacgagatca gcggctcacg
       61 atcgatctga gttcgcggaa accttgcgcc ttcggcgttg tagtccatgt cacgccagcc
      121 ggcggtgagt ggttcctcga actctcccaa gtcggcaacc ttcagcggat cggcgagtag
      181 tcagtggcgt ccatgaacac cgactcgatg cgctcgacga tcttgccgtc cacttgcgac
      241 gcttcggaga tcttcttcca ttccgggtcg gcgatgaacg ctgcccagtt ggccttggcc
      301 gcttcgcggc tggcatgcga gatcacatag atcaatgtcg tttccctggc cgggctgtcc
      361 tgcggcaccc agtacccgac attggtcatg ccgtgtttct cgaacagctt catggtgtgt
      421 tcgcggaaac gcttgtgcag ggcgtcgaga cgcccgggaa aggtgtgata cgtgcggatt
      481 tcaaacacct ttcccgcggg cgtggcgccc gtggcgagcg tcatttgcag gctcgcaccc
      541 aaaacgagcc ccgcggccat gattatccag gaacgctttg tcatcgaact gccccttcga
      601 cttgtaatta cgtctttcga ttatgcgcca ggcctcaaaa cacatggcag aatgcgcgcc
      661 catgccagaa cgtcagatgc attcgcgcac caagattgtc gcgaccctag gcccgtccac
      721 ggattccatc gaagtgctga ccgaaatggt ccgcgccgga ctcgacgtcg cgcgcgtgaa
      781 tttctcgcac ggcaaacacg aagagcacgg tcgacgcatc gacatggtgc acgaggctgc
      841 acgccgcgcc gggcgctatg tgggcatcct tgcagacctg ggcggcccca agatccgtat
      901 cgagagcttc actaacaaga aggtggatct caaggacggc caggctttcg cgctcgacac
      961 cgctcacgat atcaacgcgg gcaacgagca ggtcgtcggc tgcgcgtaca aggatctgcc
     1021 gaaggacgtg aaggccggca acatcctgtt gctgaacgac ggactcatcg agctcgaggt
     1081 cacccaggtt tccggcacgc gcatcgacac gaaagtgatc gcgggcggcg agctgtcgaa
     1141 ccgcaagggc gtgaacctca agggcggcgg catctccgcg cctgcgctca ccgacaagga
     1201 tcgcgaagac atcaagttcg cgtgcaatcg gggtgtcgat tacgtggccg tatctttccc
     1261 gcgcgacgcc gccgacatga acctcgcgcg cgatctcgtg cgcgccgccg gcggtcatgc
     1321 gcacctctgt gcgaaagtcg agcgtcacga ggccatccac aatctcaccg agatcatcga
     1381 ggcgtcggat gtgatcatgg tcgcgcgcgg cgacctgggc gtggaaatgg gctacgcggc
     1441 catcaccggc ctgcagaaaa ccatcatcca cgaatcgcgc acgcgcaata aggtcgtgat
     1501 cacggccacg cagatgatgg agtccatgat cacgagcccg gtgccgaccc gcgccgaagt
     1561 ttcggacgtc gccaatgccg tcatggacgg cacggacgcg gtgatgctct cggcggagag
     1621 cgccgtcggc aaacacccga tcaaggcgat ccaggcgatg gaagaagtca tcgccggcgc
     1681 cgagaaatac gagatcacgc acaaccgcat gcggcatcgc gccgagggca tcgtcgatcg
     1741 cagcgaagaa gccatcgcca tggcggtcat gtacacctcc aatcacatga aggtgcgcgc
     1801 catcgtcgcg ctgaccgaat ccggcaccac gccgctgtgg atgtcgcgcg cccgctcgga
     1861 catccctatc tacgcgttca cgcgccacga atcgacgcgc cgccgcgtca cgctgtaccg
     1921 cggcgtgttc ccggtgccct tcgacatccc ggtcgtgcgt accaacgagc tgttcaaggc
     1981 catcctcacg atgctgctgc agctcgagct ggtggaaaaa ggcgagctcg tcatcgtcac
     2041 caagggtgag ctcaccggca tcaccggcgg cacgaacgcg atgaccatcc tgcgcgttcc
     2101 gaaggactaa ccagtctcat gaagtggctg cgctggctgc gatggacctt gggcttcatc
     2161 gtcgtctgcg tcgccctggc agtgggaggc gcctggtggg ccgtacggca gagcctgccg
     2221 cagatcgaag ggacggcgaa cgcacccgct ctcggcaagg aagccaccat cgaccgcgat
     2281 gcgcgcggca tccccgtcat cactgccacc acgcgtgctg atctcgccta tgcgctgggt
     2341 tatgcacacg gccaggaccg cttcttccag atggacctgt cgcgccggct cgcggccggc
     2401 gagttgtccg agatgttcgg cgccgtggcg ctcaaacagg atcggcgaac gcggcgtttc
     2461 ggctttcgcg cagtggcgcg acgcgtgatc gaagcaaccc cggcggaaga gcgcgggatc
     2521 gtcgaggcat atgcgcacgg cgtcaacgcg ggcgtcgcct cgctgcacgc gcggccctgg
     2581 gaatatctgc tgttgcgcgt ccagccgcgc gaatggcttc cggaagattc cgtgctggtg
     2641 gtgcactcga tgtggtggca gctgcaatac ggcacgttgc gcgacgaggt tgcgcggcgg
     2701 cgcctggaac gcgccgcggc cgcgcgcagc gacgcccgcg ccgcgcatga gctcatcgcg
     2761 ttcgtctacg cgggccactc cgactgggac tcgcctaact acgccgccga ttcaccctgc
     2821 gtgcaggcgg cctgcaccga ttcggctggc gtgctcacgc tgtcattccc cgggcagatg
     2881 cgcttcaccg gagccgccga cgagccggac tccgagccga cagcgcccgg cagcaacaac
     2941 tgggcggtcg ccggcatcca tacccgttct ggcgtcgcac tgatcgccaa tgacatgcat
     3001 ctcgacctgg gcgtgccggc cgtgtggtat ccggcgcgct tgcgagtcac gggcaatcct
     3061 gcggtcgata tcaccggtgt cacgctgccg ggcacgcccg cggtcgcggc cgggtccaac
     3121 ggccaggtgg cctggggatt caccaacagc tatggcgact tcgccgatgc gcgctggggc
     3181 aagtgcgtga gtgccgacta tggcgtgcgc cgcgagcaca tcgcggtgaa aggcggcgac
     3241 gcgcaggagg taacgtatcg cgaagtcggt gagggggtcg tgctcgacgg cgaggaatac
     3301 gcggacgacg agaagggcgg cgaatgcctg caggcggcgt ggctcgcgac gcggcccgaa
     3361 gccaccaact tcggtctgct gcgcctcgag ggagcgcgca acatcgacga cgtactggcg
     3421 gtcgcgccgc tcgtcggaat ccccggacag aacctggtcg tcggcgacac gcgcgggcgc
     3481 attgcctgga ccttgttagg ccgggtgccc gcaaaccatg ggcccgatcg cctgttcggc
     3541 ccgatcgaat tcctggatgg tgtcgatcac ccgcgcatca ccgatccgcc ggtggggcgc
     3601 ctgtggaccg cgaatcagcg cgtggtgagc gggccgctcg aaaccgtgat gggcgacgac
     3661 gaagtcgatg tgggcgcggg cggctacgac atcggcgcgc gtgcgcgcca gatccgcgat
     3721 gacctgctga gtctccccca tccggccacc gaggccgaca tgctcgccat acaactcgat
     3781 gcgcgcgcgc tgttcgccgc gcgctggcgc gacctgttgc tggcgctcat cgatgaagac
     3841 gccatgcatg acgcgccgct gcgccgcgag tttcgcgagc tggtcagcaa gggcgcaaca
     3901 gaggccagtc ccgaggcggt tggctaccgc ctggttcgca cctaccgcag caatacactc
     3961 aatgcgatgt ggcgcagtct cgtcactgga ttgttaggtg agaaagccgt ggttcgcagg
     4021 gcagcgcagt tcgaagcggc ggggtggcgg ctggtcaacg aacgtccctc ggcggtttca
     4081 ccgccgggcg gtggtgactg gcgcgctttc ctgttgcggc gcctggacgc caccatcgcg
     4141 gaattgctga aggactgcgc gagtctggag acctgcgaat acgggcagat ggagccggtc
     4201 gaagtgagcc atccgctgtc gggtgcactg ccgctgctgc ccaggctgct cgacatgccc
     4261 acgcgtgcac tcgccggcga tcagcacatg ccgcgcgtcc agaacggctc gtttggcgcg
     4321 tcggagcgct tcgcggtgtc gcccggacgc gagcgcgatg gctatcttca gcttcccggc
     4381 gggccatccg gcaatccgct gtcgccgttc tatcgcagcg gcttcgagga ttgggccaac
     4441 ggtgtgccta cgccattcct gcccgggtcg gtagcgtacc ggctcacgct caggccctga
     4501 tcccacgccg cgtaaaaaag tgctggccgc gcgtagtccg gtagtgctac gctgtgtagc
     4561 attacaaagg gtagcaacac aatgccacac aacgtttccg acctgggtga tctcgaacgc
     4621 gaagtcctgc aactcgtgtg gggccacggt ccttcctctg cggattgggt gcaaaaggcg
     4681 ctgtcgcgcc cactcaagga atcgacggtg cgcacggtgc tcaaacggct cgaggccaaa
     4741 ggccacctca cgcatcgcgt ggatcaacgc actttcatct acgaggcggc cgacacgcgc
     4801 ggccgtgccg ccgcgcgcgc ggtgaaacgc atcgtcgacc gcttctgcag cggttcgctc
     4861 gaagaggtgc tcgtcgggct cgtcgatacg cgcatcctcg accggcgtga gctgcagcgc
     4921 ctcgcggaga aaatcgctcg cgcgcagtcg gactcggatg gccagccgaa aaggggaaag
     4981 aaatgacgcc ggaaatcctg ctgagcagcg cgttgcgcct gctgcttctt ggcgccgccg
     5041 tctggctcgc gttgcgagtc atcccggtgc gcaacccgca cgtcgaagtt ctggcctggc
     5101 ggatgatcct gatcgcgggc ctggccctgc cggcgctgct ttactggcgc ctggctccga
     5161 gtttcgcgac ttcgctcgag ctgcccgtga tcgcggcggc cggtgtgggc ggcaccaacg
     5221 caccgccggc gatggcgtcc acctcccggc tgaccgcagg cgtcctcgtg actatctacc
     5281 tgatcatcgc gctgctgctg ctcgtacgcc tcttgtcagg tctcgccgcc atgtggcgcg
     5341 tcagccgcgc ggcgcgaccg atgacgacac cagacgacgt gcgcatgagc gcgctcgttc
     5401 gcagtcccgc gactttcggc accatcatcc tgttgccagc cgacgcgcac acctggcccg
     5461 ccggcaggct ggacgccgtc ctcgcgcacg agcgcgcgca cgtgagttca cgcgacggct
     5521 tctggtcctg gctggcgcag cttcacgccg cgatcttctg gttcagcccg atcgcctggt
     5581 ggctacggcg ccgcctcgaa gcgctggcgg aaaccaccag cgacgacgcg gtggtcgctg
     5641 cacgccacga tcccgtggcc tacgccgcgc tgctgctcga tttcgcccgt caccccaact
     5701 ccaggagagt cgccatgtct gtcgcagagt cgaatgttcc aatgcgcatc gaacggcttc
     5761 tcgcgcgcac accgcccgcc accgcgttgc cgcgagtcgc acgatggagc gctttcgccg
     5821 cgctgattcc catcgctgtg tttgctgcct cgaccacgcg ggctacgcca ccggcggaac
     5881 ccgcggcgtc ggccgcggcc gcagtgccga cgcccgcgcc gattcacgtc gtgggcgtgc
     5941 gccttatccg tcccgccaat ccggacattt tctatccagc gatcgccaag cacgagaaag
     6001 tgtcgggcta cgccatagtc gaggtcgacg tggatgtgct ggggcaattg gtggatgccc
     6061 gggtgttgaa agtgcagccg accgaccccc aattcggttt tgccgacgcg gcgctgcagg
     6121 tggcccggaa cacgacatac ggaaatacga cccagcaggt cggttcgatg aagttcatgg
     6181 tgaaattcga attggcgcat tgagagtccc tcgagtgccc gccgggtggt ggccaccgcc
     6241 acccggcacg gcataagctc gcgggcatga ctgaaacccg cttcaccggc actgaacgct
     6301 acgtcgccac acccgatctc aagatggcgg tgaatgccgc cgtgaccctc ggccggccac
     6361 tcctcataaa gggcgaaccc ggcacgggca agaca
//
LOCUS       DASQJS010000183        12652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2302430, whole genome shotgun sequence.
ACCESSION   DASQJS010000183 DASQJS010000000
VERSION     DASQJS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12652
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2302430"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1360
                     /locus_tag="VEW08_07595"
     CDS             146..1360
                     /locus_tag="VEW08_07595"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HYJ40636.1"
                     /translation="MTPARDSDAAPQLTALNGMRFFAVFHIFLYHLWSVRFESPRQEG
                     PWANAYANMDGFPSWFNNLIAHGYLSTSFFFLLSGFILAYLYWSPGGELATTRQYFWW
                     QRLTRIYPAHLIALGITILLLLPRWFFDPSAPPIPLAVASAVATATLTQAWVPPLVPI
                     WSWPTWALSAVVFLYLIMPWLMSVLSKLSRGQQIGLLVASPLISLLPTIIFLQFFPDG
                     GKDSLNWQIFLGSNPLFWVAHFVAGMLMSRIFGISKFETAWRGKSKPWLSLGDLALAA
                     VVVLSLMEPHDKVWRHILRHGALLPVYMLVLYDLALGRGLVARVFSLPGMNFVGQLSF
                     SIFIWQNLFLMLGFAVLGAAPQSTSLSFWFAVIGLLVMSVISTRFIEKPLARRLRKRG
                     PTREIVSPATVT"
     gene            1444..2730
                     /locus_tag="VEW08_07600"
     CDS             1444..2730
                     /locus_tag="VEW08_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HYJ40637.1"
                     /translation="MELVRDPLLDAVTALASARDEAGVCNIVRRAARELTGADGVTFV
                     LRRGNHCYYADEDAIGPLWKGRQFPIETCISGWAMIHRQHVVIPDIYSDDRIPHDAYR
                     PTFVKSLMMMPVRREDPIAAIGAYWATYHVATPMEIGVLQSLADAAALALYNVQLDAN
                     LKEALRHEREARVSAESASRLKDEFLALLSHELRTPLHVINNWLWQLKQGKSIPPMIL
                     SRALDVIERNTALQSRLVDDLLDVSRAAAGKMSIDSQLVQVGTLCASVVELSQPAARE
                     KSIALTLTLTQKDDPRIWGDPDRLQQVLNNVVSNAIKFSTKGGRVDVQVSREGSLVSV
                     VVKDEGEGVPADFLPHMFDRFRQADASMTRRQGGLGLGLTIVREIMSLHGGTVRAESA
                     GPGKGTTIYLEFAVAEEPQAGSDVHRPSLHPKAPVH"
     gene            2731..3093
                     /locus_tag="VEW08_07605"
     CDS             2731..3093
                     /locus_tag="VEW08_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ40638.1"
                     /translation="MKTTTVLVAEDYDDARDIMSMVLQSAGFQVLEARDGIEALEAAR
                     SFRPTVIVMDMFMPNMDGLTATRALRDEPALRRIPVIAQTAQPSVISGSRELFDAVLA
                     KPCSPDLLIRTISELIAR"
     gene            complement(3142..4263)
                     /locus_tag="VEW08_07610"
     CDS             complement(3142..4263)
                     /locus_tag="VEW08_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYJ40639.1"
                     /translation="MTTPAASQGRFGAAFEWLGIERAWHRTRGSADVVIGIVDEGVQV
                     DHPLLGPNIQRDLAHNPASGGSHEIAGTHAAGVAAGRFSEVERFSGVAPDARILPVRF
                     SNGIGPQALDLAHAIEYAVEMGACIINVSPTGEVAESSVQRAIQYAAARNVLVVCSAP
                     DAISPMALADEDRVPNQINVLSIGDQWQPMVICAHQSAHLAAPGFARVPQWRGSGHDT
                     LLGNAIGAAYVSGCAALVKALNPTWGYHEIKEHLLASCIVHPELEGKCRTAAFLNVAN
                     AVLGPIELAGDARPITWQSLSDVVLEWKLRYRAPLCAKVAALFRPHGDAHWRELGVAR
                     ASALKMTIPGHELRRSSGTLRIACRESNFSTEEVALTIA"
     gene            complement(4306..4935)
                     /locus_tag="VEW08_07615"
     CDS             complement(4306..4935)
                     /locus_tag="VEW08_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40640.1"
                     /translation="MNQEQIEQYIAGRMGEAEALAFEEYCLSNPEFARQVEYEQRLKA
                     GLAQVASGSTAEFVRSEHPLRWSLAAAAGLLVAFIAVISIWTSGLHAPVPTIMAAVTD
                     SSPQGDSSLRLALVRGSDTAPALPPGLVRVEIVGLFDAGLFYSVALDRREQNQKIDTI
                     ATLNDQRPTSPMSLEVMIDSKQLRAGSYSLRVRKQASGEEALDFGFAKF"
     gene            complement(4932..5525)
                     /locus_tag="VEW08_07620"
     CDS             complement(4932..5525)
                     /locus_tag="VEW08_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ40641.1"
                     /translation="MDFIPGGELSRRIRDGDVSAESELIRQFEPGLRVLLRRRTGGDY
                     GLLQDLVQETLLVVIQRLRGEGIDDPQKLAAFAAQTARNLAIASLRKAERQKTDVDSD
                     ATDRNADPTQSVDRIASDLEAALAVRALLRELPQARDRLMLKRYYLDDHDKDLICQEL
                     DLSEAAFNQALSRARRRFRQILEERGFAKRDLLSLGL"
     gene            5627..6019
                     /locus_tag="VEW08_07625"
     CDS             5627..6019
                     /locus_tag="VEW08_07625"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HYJ40642.1"
                     /translation="MLKTYHGSCHCGAVRFEADIDLTQPTYRCNCSICSRNRFWAAVV
                     TPGAFRLLEGKSAVQQYLFNSMRNEHYFCKTCGVRAYGFGNKGEMVGVNIGCLEDTTP
                     EELAAAPIRHCDGKHDNWQNPPAITSYL"
     gene            complement(6003..7265)
                     /locus_tag="VEW08_07630"
     CDS             complement(6003..7265)
                     /locus_tag="VEW08_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ40643.1"
                     /translation="MNSLPQPNPADSADSDDSPSAERRNLADALAHERNVLRTMIDLI
                     PAFIYAKDAQSRFTAANKLVANRMGVEPADLIGKTDFDFFPREHAEKFFADEQALIRS
                     GVPLIDHEELAFDKTRGKDRVILTSKIPLRDEAGNLTGIVGTGLDITDRKDAEARMAS
                     SDRLESIGRLAAGVAHEINTPIQYLNDSVTFIRDGVGELLVYIDELHKATDARPASSE
                     DVDYMREELPPALTRVAEGLSRITEIVRSMKVFSHADQREMSEVDLNCSISSTLVVAR
                     SEYRDVADVETDFGEIPRVTCHGGQINQVVLNLVVNAAHAIGDQIKKGGTRGRISIRT
                     HEENGRVVISIADTGGGIPEAIRAHIFDPFFTTKEVGRGTGQGLSVSRNVVIKGHGGE
                     LDFITEPGKGTTFFVRLPIGGAPSTSSS"
     gene            complement(7360..8019)
                     /locus_tag="VEW08_07635"
     CDS             complement(7360..8019)
                     /locus_tag="VEW08_07635"
                     /inference="COORDINATES: protein motif:HMM:NF018749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HYJ40644.1"
                     /translation="MSPTRRIFLQQSAGVALVAHAGATAAAESASARTGGDLLSRMTW
                     LNPPATEHYSQGVLTVRSKGKTDFWRKTFYGYVTDNGHFMHVPLIGEFMMQARINGNY
                     SALYDQAGLMVRLDDKHWMKCGSEFVEGKRWASVVFTHDYSDWSTMEDLSQSGAVWWR
                     VVRKKDSIEAQCSKDGEKFLTIRQGYFPADVKVMAGVMCAAPEGAGFDAVFDQLTIEK
                     A"
     gene            complement(8034..8648)
                     /locus_tag="VEW08_07640"
     CDS             complement(8034..8648)
                     /locus_tag="VEW08_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HYJ40645.1"
                     /translation="MNVRNVVFDMGGVLLEWNPVRTIAELYPDAATQAVIRQQMFEHA
                     DWHEFDRGSLSYEVAIQHFAKQTGRSPDETRALIHATRESLTPIAGSIKLLEELAAAG
                     VHLYLLSNMPVSTFDYLIRQHQFFSHFKHLVISGAILLVKPEPAIYKHLVEQTGIVPA
                     ESVFIDDLLKNVIAARECGFHAIQFSDPESCREQLRTYLPRIPL"
     gene            complement(8713..9336)
                     /locus_tag="VEW08_07645"
     CDS             complement(8713..9336)
                     /locus_tag="VEW08_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HYJ40646.1"
                     /translation="MALNTFLLYLATWTLVALSPGPAVFFSMSQAARHGMRGAAAGTA
                     GILLGHVLVFAAVALGLAALLASYGGAVTTIRIVGAVYLMYLGAKMLFSKPREAQAIA
                     AVPAPPAHGGLVLQGLVVQLTNPKMLLFVLALLPQFISPDYPLPLQLLIMLTITVVID
                     GIALLAYAKLASHGARALKGSRALAWLERAFGGALIYFGLNLLVARK"
     gene            9433..10431
                     /locus_tag="VEW08_07650"
     CDS             9433..10431
                     /locus_tag="VEW08_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40647.1"
                     /translation="MIVPKYWAEGRAHHREKGKQVTVRRYGWSDASQADAQTSADARA
                     QEALARVLSGEKLHRRDHKVAYNGAEGLPIREEILSTHGDAVITRNSYGAHCLNTPNV
                     LFADVDFGESLWWKSTSYLIAAAITMFVFLWMTADCCEERSSRFWLFVGTLIGTAIVC
                     RKLSEWLRKLLLNRAGGAANATQNRIRGFAARHPDWGLRVYRTPAGFRVVASHATFTA
                     SQPEVAECFSSLRVDRIYVRMCLNQQCFRARLTAKPWRMGMSGHMRPRPGVWPIKPEQ
                     VPVRAAWVAEYERVANGFAACRFIEKLGNAIVHPDVRSVIELHDAQSRAHSELPLA"
     gene            complement(10493..11926)
                     /locus_tag="VEW08_07655"
     CDS             complement(10493..11926)
                     /locus_tag="VEW08_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYJ40648.1"
                     /translation="MSDLTFDRRAMLKTAGAAAVGLSAIATGATPRRKFVNVGLGSRS
                     RMYLNAITKTFAEGNELVGICDVNLGRLDVAAKLVAPNGGKPKKYLATDFDKMLKELK
                     PDCVIVTCPDAFHDDYIVRALDAGCDCITEKPLTTTPEKAQRIVDACKRNNRHVRVLF
                     NYRYSPPRTQVKDLLMSGAIGDVMSVDFHWLLNTLHGADYFRRWHSQKEISGGLMIHK
                     ATHHFDLVNWWLGSEPEIVQAYGKREFYTPAMAKRMGLESHHERCLTCPEKAKCTFYL
                     DIGADRNFKELYLDNEKYDGYFRDRCVFRPEIDIEDTMNVIVKYKTGATLSYSLNAFN
                     AWEGYTIAFNGTKGRIEHSIVEGGAVSAGATNYQGTDSDRERTRVIPLRGPAQDLEVW
                     TGKGGHGGGDTVMLTEVFGTSEPDKYKRAADERSGLYSCLIGASANKSFLSGNAVKVA
                     DLVTGLTSPMVAPMPTRNDPVPMPIKA"
     gene            12046..>12652
                     /locus_tag="VEW08_07660"
     CDS             12046..>12652
                     /locus_tag="VEW08_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40649.1"
                     /translation="MFPLRVFICLLTTLVVPQFATAAPIDRHALVTRHNPTITAVDKS
                     APFMVGNGNLAFTADITGLQTFPEQYSTLVPLMTQAQWAWHSFPNPKGFTLAQAEVPI
                     KVRGKTQKYPYLSDWEQAKRPEVQWLRENPHRFSLARLGLYLANTGGKPVPFSDLSAT
                     RQTLDMWTGRLSSSFVFDGVPVDVESSVHPERDLIIVRLRSN"
BASE COUNT         2254 a         3927 c         4015 g         2456 t
ORIGIN      
        1 tgctcctgcg atgtcttgtg aacgggggtg gatggtaagg accagggcgc ccacgcccca
       61 tcagcccatg tcccgcccca gtgtcagacc ccatgcaacc ggatctccat ccggtgtgta
      121 taaaatgcaa tcggggagaa ctaccatgac gcctgcacgc gattccgatg ctgcgccaca
      181 gctcactgcc ttgaatggca tgcgcttctt tgcggtattc cacatctttc tgtaccacct
      241 gtggtctgtc cgcttcgaat cgccgcgcca ggaggggccc tgggctaatg cctacgccaa
      301 catggacggc ttcccgtcct ggttcaacaa cctgattgcg cacggctatc tatccaccag
      361 cttcttcttc ctgctctcgg gattcatcct ggcgtacctg tactggtcgc ccggtggcga
      421 actggccacg acgcggcaat atttctggtg gcagcgcctg acgcgaatct atcccgcgca
      481 cctgatcgcc ctcggcatca cgatcctgct tttgctgccg agatggttct tcgatccatc
      541 cgcaccgccg attccgctcg ccgtcgcgag cgccgtggcc acggcgacgc tgacgcaggc
      601 gtgggtgccg ccgctggtgc cgatctggag ctggcccacc tgggcgttgt ccgcggtggt
      661 gtttctttac ctgatcatgc cctggctcat gagcgtgctg tcgaagctct cgcgtgggca
      721 gcagatcggc ttgttagtgg cgagcccgct gatttcgctc ctgcccacga tcatcttcct
      781 gcagttcttc cctgatggcg gcaaggacag cctgaactgg cagatcttcc tcggctcgaa
      841 tccgctgttc tgggtggcgc acttcgtcgc gggcatgctg atgtcgcgca ttttcggcat
      901 cagcaagttc gagaccgcct ggcgcggcaa gtcgaagccg tggctgtcgt tgggcgacct
      961 ggcgctcgcc gcggtagtgg tgctgtctct catggagccg cacgacaagg tatggcgcca
     1021 catcctgcgc cacggcgcgc ttctgccggt gtacatgctg gtgctgtacg acctggcgct
     1081 gggccgcggt ctcgtcgcac gcgtgttttc gttgccagga atgaacttcg tgggccagct
     1141 ctcgttctcc atcttcatct ggcagaacct gttcctgatg ctgggctttg cggtactcgg
     1201 cgcggcaccg cagtcgacca gcctgtcatt ctggtttgcc gtgatcgggc tgctcgttat
     1261 gtccgtgatc agcacacgct tcatcgagaa gccgctggct cgacgcttgc gaaagcgcgg
     1321 tccaacccgg gagatcgtga gccccgcaac ggtgacctga aaatttcgca gggagtcacc
     1381 gattgcccca gtgtcgcaac acaggcattc tgaccacgcc ccaatcaacc cgagcccgat
     1441 aatttggagc tggtccgcga tcccctcctg gatgccgtca ctgccctagc ttccgcgcgg
     1501 gacgaggcag gcgtctgcaa catcgttcgc cgggcagcgc gcgagctcac gggcgcggac
     1561 ggcgtcacct tcgtgctccg ccggggaaat cactgctact acgccgacga agacgccatc
     1621 gggccgctgt ggaaaggccg gcagtttccg atcgagacct gcatctccgg ctgggcgatg
     1681 atccatcgcc agcatgtggt gatcccggac atctactccg acgatcgcat cccgcatgat
     1741 gcctatcggc ccaccttcgt caaaagcctg atgatgatgc cggtgcggcg tgaagacccg
     1801 atcgccgcca ttggcgccta ctgggctacc tatcacgtgg ccacacccat ggagatcggc
     1861 gtgctgcaga gcctcgcgga tgccgccgcg ctcgcgctgt acaacgtgca gctcgatgcg
     1921 aacctgaaag aggcgttgcg tcacgaacgc gaagcgcggg tatcggccga gagcgcgagc
     1981 cggctcaagg acgaattcct ggcgttgttg tcgcacgagc tgcgcacacc gctgcacgtg
     2041 atcaacaact ggctctggca gctcaagcag ggcaagtcga taccgccgat gatcctgtcg
     2101 agggcgctcg atgtcatcga acgcaacacg gcgctgcagt cgcgcctggt cgatgacctg
     2161 ctggacgtgt cgcgtgcggc tgcgggcaag atgtcgatcg actcacaact cgtgcaggtc
     2221 ggcacgctgt gtgcgtccgt cgtggagctg agccagccgg cggcaaggga aaagtccatc
     2281 gcactgaccc tgaccctgac ccagaaagac gatccccgga tctggggtga tccggaccgg
     2341 ctgcagcagg tgctcaacaa cgtggtgtcg aacgccatca agttttcgac gaagggcggc
     2401 cgcgtggatg tgcaggtatc gcgcgaaggt tcgctggtca gcgtggtggt gaaggatgaa
     2461 ggcgagggcg taccggctga tttcctgccg cacatgttcg atcgctttcg tcaggccgat
     2521 gcgagcatga cgcgccggca gggtgggctc ggtctggggc tgactatcgt gcgcgaaatc
     2581 atgtccctgc atgggggcac ggtgcgcgcg gaaagcgcgg gaccgggtaa ggggacgacg
     2641 atctacctgg agtttgcggt cgccgaagag ccgcaggccg gcagtgatgt gcatcgcccg
     2701 tcgttgcacc cgaaagcgcc ggtgcattga gtgaagacca cgacggtgtt ggtcgcggaa
     2761 gactacgacg acgcgcgcga catcatgtcc atggtgctgc agtcggcggg attccaggtg
     2821 ctggaagccc gcgacggcat cgaggctctg gaagcggcgc gcagttttcg cccgacggtc
     2881 atcgtgatgg acatgttcat gccgaacatg gacggtctga ctgccacgcg cgcactgcgt
     2941 gacgagcctg cgctgcgacg cattccggtg attgcgcaaa cggcgcagcc gagcgtgatc
     3001 agcggttcgc gggagttgtt cgatgcggtg ctcgccaagc cctgttcgcc cgacctgctg
     3061 atccgcacta tctccgagct gattgcccgc tgagaaatgg tgccaggcac cgtttcctag
     3121 gaaacggtgc ctgaccccat ttcaggcaat cgtcagcgcg acttcctcgg tcgagaagtt
     3181 ggactcgcgg cacgcgatgc gaagcgtgcc cgaagaccgg cgcaactcgt ggccggggat
     3241 ggtcatcttc agcgcgctgg cacgcgcgac gccgagttcg cgccagtggg cgtccccatg
     3301 cggacgaaac agcgccgcga ccttggcgca cagcggcgcg cgataacgca gcttccattc
     3361 gagcaccacg tcgctgagcg actgccacgt gatgggccgc gcatcgcctg cgagttcgat
     3421 ggggcctaac acggcgttgg ccacgttgag gaaagctgcc gtccggcact tgccttcgag
     3481 ctcgggatgc acgatgcagc tcgcgagcag gtgctccttg atctcgtgat agccccacgt
     3541 gggattcagt gccttgacga gcgcagcgca gccgctcacg tacgccgccc cgatcgcgtt
     3601 gccgagcagt gtgtcgtgtc cgctgccgcg ccattgcggc acgcgcgcaa acccgggcgc
     3661 ggccaggtgt gcggactggt gcgcgcaaat caccatcggc tgccattggt cgccgatcga
     3721 cagcacgttg atctggttag gcacgcggtc ttcgtcggcc agcgccatcg ggctgatcgc
     3781 gtcgggcgcg gaacacacca ccagcacgtt gcgcgcggcg gcgtactgga tggcccgttg
     3841 caccgacgat tcggcgactt cacccgttgg cgatacgttg atgatgcagg cgcccatttc
     3901 caccgcgtat tcgatggcgt gcgccagatc cagtgcctgc gggccgatgc cgttgctgaa
     3961 cctgaccggc aggatgcgcg catcgggcgc gacgccggaa aagcgctcga cttcgctgaa
     4021 gcgcccggcc gcgactccgg cggcgtgtgt gccggcgatc tcatggcttc cgcccgatgc
     4081 cgggttgtgt gcgagatcgc gctggatgtt gggaccgagc aggggatggt cgacctgcac
     4141 gccttcatcg acgatgccga tgactacgtc ggcgctaccg cgagtcctgt gccaggcgcg
     4201 ctcgatcccg agccactcga aggcggcgcc aaagcgcccc tggctggccg caggagtcgt
     4261 cataaatgag cttctgcgac gagatcgcca gccgtgacac cgatctcaga actttgcgaa
     4321 gccgaaatcc agcgcttcct cgcccgaggc ctgtttgcgc accctgagcg aataagaccc
     4381 agcgcgaagt tgtttgctgt cgatcatcac ttcgaggctc atgggcgagg tcggacgctg
     4441 gtcgttgagc gtggcgatgg tgtcgatttt ctggttctgc tcgcgccggt cgagcgcgac
     4501 gctgtagaaa agaccggcgt cgaacaaccc gacgatttcg acccggacga gcccgggcgg
     4561 aagggccggt gcggtatcgg agccgcgaac cagcgccagg cgcagcgacg agtcgccctg
     4621 cggcgagctg tcggttacgg ccgccatgat ggtggggact ggcgcatgca gtccggaagt
     4681 ccagatagag atcaccgcaa tgaacgcaac cagcagtccg gctgcggcag ccaggctcca
     4741 gcgcagcgga tgttcggagc gcacgaattc cgccgtggaa ccgctggcaa cctgcgccaa
     4801 cccggccttc agtcgctgct catattcgac ctggcgcgcg aattcagggt tcgaaaggca
     4861 atactcttcg aaggcaagcg cttcggcttc gcccatgcgt ccggcgatgt actgctctat
     4921 ttgttcctga ttcataaacc gaggctcagg agatcgcgct tggcgaagcc ccgttcttca
     4981 agaatctgcc ggaatcgccg ccgggcacgg ctcaacgcct ggttgaacgc cgcttcggac
     5041 aagtcgagtt cctgacaaat caagtctttg tcatggtcat ccagatagta gcgtttgagc
     5101 atcaggcgat cccgggcttg cggcaattcg cgcaacagcg cgcggaccgc gagcgccgcc
     5161 tcgaggtcgg acgcgatcct gtcgacactc tgcgtcggat ccgcatttcg atccgtagca
     5221 tcgctgtcga catctgtctt ctgtcgctca gccttcctca gtgacgcgat tgcgagattt
     5281 cttgcagtct gtgccgcaaa agccgcgagc ttctgggggt cgtcgatgcc ttcgccacgt
     5341 aggcgctgga tgacaaccag cagagtctcc tgaaccaggt cctgcaatag gccgtaatcg
     5401 ccgcccgtac gacggcgcag aagcacccga aggccgggtt cgaactgtcg aatgagttcg
     5461 ctttcggcgg agacatctcc gtcccgaatt cgacgcgaca gctcgccgcc aggaatgaaa
     5521 tccataccct aaatttcctt ggaccccgcg ctcttcgatt atcagatagg agcggggtag
     5581 gattctgcca cggcccggtc gcgcaatctg agaagtaggg aatcctgtgc tcaagacata
     5641 tcacggctcc tgccactgtg gcgccgtacg ctttgaggcg gatatcgacc tcacgcagcc
     5701 cacttatcgc tgcaattgct cgatttgctc gcgaaatcgc ttctgggcgg cggtggtcac
     5761 gcccggcgca ttccggctcc tggagggcaa atcggcggtc cagcagtacc tgttcaactc
     5821 catgcggaac gagcactact tctgcaaaac ctgtggagtc cgtgcctacg gcttcggcaa
     5881 caagggcgag atggtgggcg tcaacatcgg ctgcctggaa gacacgacgc ccgaggagct
     5941 ggcggcggcg cccattcgcc actgcgatgg caaacacgac aactggcaga acccgccagc
     6001 gatcacgagc tacttgtaga cggcgctccg ccgatcggca ggcgcacgaa gaaagtggtg
     6061 cctttgccgg gttcggtgat gaagtcgagc tcgccgccgt ggcctttgat cacgacgttg
     6121 cgtgacacgg agagtccctg gcccgtgccg cgtcccactt ccttggtggt gaagaacgga
     6181 tcgaagatgt gtgcgcgtat cgcttcggga atgccgccgc ccgtatcggc gatcgaaatg
     6241 accacgcggc cgttttcttc gtgcgtgcga atgctgatcc ggccgcgcgt gccgcctttc
     6301 ttgatttgat cgccgatggc gtgcgccgca ttcaccacga ggttcagcac tacctggttg
     6361 atctgtccgc cgtgacaggt cacgcgcgga atttcaccga agtcggtttc cacgtcggcg
     6421 acgtcccgat attcactgcg cgccaccacc agcgtgctgc tgatggagca attcagatcc
     6481 acttcgctca tctcgcgctg gtcggcgtgc gagaagacct tcatcgaacg cacgatttcc
     6541 gtgatgcgtg agaggccttc cgccacgcgc gtgagcgccg gcggcagttc ttcgcgcatg
     6601 tagtccacgt cttcgctgga cgcaggtcgc gcgtcggtgg ccttgtgcag ctcatcgatg
     6661 tacacgagca actcgcctac gccatcgcgg atgaaggtca cgctgtcgtt gaggtattga
     6721 atcggcgtgt tgatctcgtg cgcgacgccc gcggccagcc ggccgatgga ttcgagccgg
     6781 tcgctcgagg ccatccgcgc ttcggcgtcc ttgcgatccg tgatgtcgag gcccgtgccg
     6841 acgatgccag tgaggttgcc ggcctcgtca cgcagcggaa tcttggacgt gaggatgacg
     6901 cggtccttgc cgcgcgtctt gtcgaaagcg agctcctcgt gatcgattag aggaacgccg
     6961 gagcggatca gcgcctgttc gtccgcgaag aatttctcgg catgctcgcg cggaaaaaaa
     7021 tcgaaatcgg tcttgccgat gaggtcggca ggctcgacgc ccatgcgatt cgcgaccagc
     7081 ttgttggcgg ccgtgaagcg gctttgcgcg tccttcgcgt agatgaaggc ggggatcagg
     7141 tcgatcatcg tccgcaacac gttgcgctcg tgcgcgagcg cgtccgcgag attgcgacgc
     7201 tcggcgctgg ggctgtcgtc cgagtctgcg gaatcggccg gattgggttg cggaaggctg
     7261 ttcacggccc tgggtgtaac ggcctccggg ccttgatctg tagtagtagg ggcggaaagg
     7321 tggattggct cacagacggg ccgaccgccg tgatcgaagt taagcctttt cgatcgtaag
     7381 ttgatcgaaa accgcgtcga agcccgcacc ttctggcgca gcgcacatca cgcccgccat
     7441 cactttcacg tcggccggga aatagccctg gcggatggtg aggaattttt cgccatcttt
     7501 cgagcactgc gcttcgatgg agtctttctt gcgcacgact cgccaccaga ccgcgccaga
     7561 ttgggacaag tcttccatcg tcgaccagtc ggagtaatcg tgcgtgaaaa ccacgctcgc
     7621 ccagcgcttg ccctcgacga attcggaacc gcacttcatc cagtgcttgt cgtcgaggcg
     7681 caccatgagt cccgcctggt catagagcgc ggaatagttg ccgttgatgc gcgcctgcat
     7741 catgaactcg ccgatcaacg gcacgtgcat gaagtggccg ttgtcggtga cgtacccgta
     7801 gaaagtcttg cgccagaaat cggtcttgcc cttcgaacgc accgtgagga cgccctggct
     7861 gtagtgctcg gtcgccggcg gattgagcca ggtcatgcgg ctcagcaggt cgccaccggt
     7921 gcgcgccgaa gcgctctcgg ctgccgcggt cgcgccggca tgcgccacca gcgcgacgcc
     7981 ggccgactgc tgcaggaaaa ttcttcgggt gggactcatg agctctctcc tggtcataac
     8041 ggaatccgcg gtagataggt gcgaagctgt tcacggcagc tttccggatc gctgaactgg
     8101 atggcatgga agccgcactc gcgcgcggcg atcacgttct tgagcaagtc gtcgatgaac
     8161 acgctctcgg cgggcacgat gccggtctgc tccaccaggt gcttgtagat cgccggctcc
     8221 ggcttcacca gaagtatcgc gccggagatc accaggtgtt tgaagtggct gaagaactgg
     8281 tgttgccgga tgaggtaatc gaacgtgctg accggcatgt tcgacagcag gtacagatgc
     8341 acgccggcgg cggcaagctc ttcgagcagc ttgatgctgc ccgcgatggg cgtgagcgac
     8401 tcgcgggtgg cgtgaatcag cgcacgcgtc tcatccgggg aacgaccggt ctgtttcgcg
     8461 aaatgctgaa tggcgacttc atagctgagg gagccgcgat cgaattcgtg ccagtcggcg
     8521 tgttcgaaca tctgctggcg gatgaccgcc tgcgtggcgg catcgggata aagctccgcg
     8581 atggtgcgga caggattcca ctcgagcagc acaccgccca tatcgaacac gacgttccgg
     8641 acattcatgt ccggattatg gcgggagaaa aggtgccagg caccgtttcc tggaaaacgg
     8701 tgcctggcac cattatttgc gggcgacgag caggttcagg ccgaagtaga tgagcgcgcc
     8761 gccgaaagcg cgttccagcc atgcgagggc gcgcgagcct ttgagcgcac gcgcgccatg
     8821 cgatgcgagc ttcgcgtacg ccagcagcgc gatgccgtcg atgaccacgg ttatcgtcag
     8881 catgatcagg agctgcaacg gcaacgggta gtccgggctg atgaactgcg gcaacaacgc
     8941 cagtacgaac agcaacatct tgggattggt gagctgcacc acgagaccct gcaacaccag
     9001 cccgccgtgc gctggcggtg ccggtacggc ggcaatggct tgcgcctcgc gcggcttcga
     9061 gaacagcatc ttcgcaccga gatacatgag gtagaccgcg ccgacgatgc gaatcgtcgt
     9121 gactgcgccg ccataactcg ccagcagggc cgcgagaccc agcgcgaccg cggcgaacac
     9181 gaggacgtgg cctagcagaa ttcccgcggt tcccgccgcc gcgccgcgca tgccgtggcg
     9241 cgccgcctgc gacatggaaa aaaagaccgc agggccgggc gagagcgcga ccagcgtcca
     9301 ggtcgcgaga tagagaagga aagtgttgag tgccatggtc aataggttag taaagattca
     9361 ccgggcctgt cgcgagtcac tttgtgggat attcgcggcc accgtattgg gggtagttgg
     9421 agtgcggcac gaatgatcgt tcccaaatat tgggcggaag gccgggcgca ccatcgcgag
     9481 aagggcaaac aggtgaccgt ccggcgttat ggctggtcag acgcgagcca ggcagatgcg
     9541 caaaccagcg cggatgcgcg agcccaggaa gcgttggccc gcgtgctgtc cggcgaaaag
     9601 ctgcatcgac gggaccacaa ggtcgcctac aacggcgccg aaggcctgcc gattcgtgaa
     9661 gagatcctgt cgactcacgg cgacgccgtc atcacgcgca attcctatgg cgctcattgc
     9721 ctcaacacac ccaacgtcct gttcgccgat gtggatttcg gggaatccct ttggtggaaa
     9781 agcacttctt atctgattgc cgcggcgatc accatgttcg tcttcttgtg gatgacggcc
     9841 gactgttgtg aggagcggag ctccagattc tggttgttcg ttggaaccct catcggcaca
     9901 gcaatcgtct gcaggaaatt gagtgagtgg ctgcggaagc tcctgctgaa tcgggcgggc
     9961 ggggcagcaa atgcaacaca gaaccgcatt cgcgggtttg ctgcccgcca tccggattgg
    10021 ggcctgcgcg tctatcgcac accggccggc ttccgcgtgg tggcctcgca cgccacattc
    10081 acggcgagtc agcccgaagt ggcggaatgc ttctcgagcc tgcgagtcga ccgcatctat
    10141 gtgcgcatgt gcctcaatca gcagtgcttt cgcgctcggc tgactgcgaa gccgtggcgc
    10201 atggggatgt cgggacacat gcggccacgg cccggtgtct ggccgatcaa gcctgagcag
    10261 gttccggtgc gcgctgcctg ggtggccgaa tacgagcgag tcgcgaacgg attcgcggcg
    10321 tgccgcttca tcgagaagct cggcaacgcc atcgtgcatc ccgatgtccg ttccgtcatc
    10381 gagctgcacg acgcgcaaag ccgtgcgcat agcgagctgc cgctcgcttg acggtgccgg
    10441 gtggggattg tggggagtgg actccccaca atccccaccc ggcaccaacc cactacgcct
    10501 tgatgggcat cggcaccggg tcattgcgcg tcggcatcgg cgcgaccatg ggcgaggtca
    10561 agccggtcac gaggtcagcg accttcacgg cgttgccgct caggaaactc ttgttcgcgg
    10621 acgcgccgat gaggcacgaa tacaggccgc tgcgctcatc ggcggcgcgc ttgtacttgt
    10681 cgggctcgga cgtgccgaac acttccgtca gcatgacggt gtcgccgccg ccgtggccgc
    10741 ccttgccggt ccacacctcg aggtcctgcg ccggcccgcg caacgggatc acccgcgtgc
    10801 gctcgcgatc gctatccgtg ccctggtagt tagtggcgcc ggccgagacc gcgccgccct
    10861 cgacgatcga gtgctcgatg cggcccttgg tgccgttgaa cgcgatcgtg taaccctccc
    10921 acgcattgaa cgcgttcagc gagtacgaca gcgtggcgcc ggtcttgtac ttcacgatga
    10981 cgttcatggt gtcttcgatg tcgatttccg gccggaacac gcagcgatcg cggaaatagc
    11041 cgtcgtactt ctcgttgtcg agatacaact ccttgaagtt cctgtcggcg ccgatgtcca
    11101 ggtagaacgt gcacttcgcc ttctcgggac aggtgaggca gcgttcatgg tggctctcga
    11161 gccccatgcg tttcgccatg gccggcgtgt agaactcgcg tttgccgtag gcctgcacga
    11221 tctcgggctc gctgcctaac caccagttca ccaggtcgaa gtggtgcgtc gccttgtgga
    11281 tcatcaggcc accggagatt tccttctggc tgtgccagcg gcggaaatag tccgcgccgt
    11341 gcagagtgtt caacagccag tggaaatcca ccgacatcac gtcgccaatg gcgccgctca
    11401 tgagcaggtc cttgacctgc gtgcgcggcg gcgaataacg gtagttgaac aacacgcgca
    11461 cgtggcggtt gttgcgcttg caggcgtcga cgatgcgctg cgccttctcc ggcgtcgtgg
    11521 tcagcggctt ttcggtgatg cagtcgcagc cggcgtccag cgcacgcacg atgtaatcat
    11581 cgtgaaaggc gtcggggcag gtgacgatga cgcagtcggg cttcaattcc ttcagcatct
    11641 tgtcgaaatc ggtggcgaga tattttttcg gcttgccacc gttgggcgcg acgagcttcg
    11701 cggccacatc gaggcggccc aggtttacgt cgcagatgcc gacgagctcg ttgccttcgg
    11761 caaaggtctt ggtgatcgcg ttgaggtaca tgcgcgaacg cgagcccagg ccgacgttca
    11821 cgaacttgcg gcgcggtgtg gcgcctgtgg cgatggccga aaggcccacg gccgcggctc
    11881 cggcggtctt gagcatggcg cgtcggtcga aagtcagatc actcatctat atgtccccca
    11941 cgttcgtcgt gacgtacgat ccgatgcgaa gatacctcaa tttcgatcaa cagcgatcac
    12001 gggcgcgcac gcccaacatc aattttcagc acatactttg ccgccatgtt tccactccgc
    12061 gtattcatct gcctgcttac gactttggta gtgccgcagt ttgcgactgc cgctcccatc
    12121 gaccggcatg cgctcgtcac ccgccacaac cctacgatca ccgcggtcga caagtccgca
    12181 cccttcatgg tgggcaacgg gaatctggcg ttcacggccg acatcaccgg cctgcagacc
    12241 tttcccgagc aatattcgac gctcgtgccg ctcatgaccc aggcgcagtg ggcctggcac
    12301 agcttcccga atccgaaagg cttcacgctt gcgcaggctg aagtgcccat caaggttcgg
    12361 ggcaagacgc agaagtaccc ttacctgagc gactgggagc aggcaaagcg gcccgaggtg
    12421 cagtggttgc gcgagaaccc gcatcgtttt tcactggcgc ggctcggcct gtatctggcg
    12481 aacaccggcg gcaagccggt gccgttcagc gatctatccg cgacccgcca gaccctcgac
    12541 atgtggacgg gccggctttc gagcagcttc gtgttcgatg gcgtaccggt ggatgtcgaa
    12601 tcctccgttc atccggagcg cgatctcatc atcgttcgct tgcgctcgaa cc
//
LOCUS       DASQJS010000184         5281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_103571, whole genome shotgun sequence.
ACCESSION   DASQJS010000184 DASQJS010000000
VERSION     DASQJS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103571"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /gene="kaiC"
                     /locus_tag="VEW08_07665"
     CDS             <1..1308
                     /gene="kaiC"
                     /locus_tag="VEW08_07665"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HYJ40650.1"
                     /translation="NGAQQFAEPGVLMTFEENAAELAQDVRSLGYNLDSLVAQKKLIV
                     DYVHVDRSEIEETGEYDLEGLFVRLEHAIKEIGARRVMLDTVETLFGGLKDTGILRAE
                     LRRLFRWMRDRGITAVVTAERGEQMFTRQGLEEYISDAVISLDHRVHEQISTRRIRVV
                     KYRGSTHGTNEYPFLIDRDGITVLPVTSLLLEHEAPNERISTGLKSLDDMLGGKGYFR
                     ASTVLLSGTAGTGKTTLAAQFIEAACARGEKCMYFLFEESPSQLIRNMRSAGIDLQKW
                     LDKGLLKFHADRPNRHGLETHLLEMHHAVEVFDPDVVVLDPITNLMAVGTQSDVRAML
                     TRVIDYFKTRGVTAVFTSLTSSTQSLEATESMISSLMDTWILVAVAEEARKRNRWIYV
                     LKSRGMPHSNDVRAFEITDHGVAITPLGRNGLAGKPKPARKRK"
     gene            1305..1595
                     /locus_tag="VEW08_07670"
     CDS             1305..1595
                     /locus_tag="VEW08_07670"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HYJ40651.1"
                     /translation="MNENYALKLYVAGQTPKAVRAFSNLRKICEEHLQGRYSIEVVDL
                     IENPALGRGDQILALPTLVRRLPTPIKKIIGDLSNTERVLVGLDLRPRRPST"
     gene            1592..1927
                     /locus_tag="VEW08_07675"
     CDS             1592..1927
                     /locus_tag="VEW08_07675"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HYJ40652.1"
                     /translation="MNETPLHTLADLADQAERQPYVLRLYVTGMTPRSTRAISAVRAI
                     CEEFLAGRFDLKIIDVYQQPALIRDEQIFATPTLVRKGPEPERRMIGDMSNRARLLAG
                     LGLLEVTPA"
     gene            1924..3543
                     /locus_tag="VEW08_07680"
     CDS             1924..3543
                     /locus_tag="VEW08_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ40653.1"
                     /translation="MNIEDLDIEELRARLVEAEGNLQAIISGGIDALLLADENGERRV
                     FTLRSADAPYRALVERMQEGAATLSIGGDIVYCNQRFADLVQMPLEQVFGAPIGKFLP
                     AAEGEILRAMLEHGSGRYQTRLVLGGQESTEVQVTFSSIVLDDVEHRTLILSDISSLA
                     RAQRENRSKDEFLAMLAHELRNPLGAIQGAVHALALMNQTEPMALRATGIIKRQVVHM
                     ARLVDDLLDVGRAVTGKIVLKRVPVNLAEAVKASISALASGNASHQGRIDLVSEPVWV
                     SGDPVRLEQIVGNLVSNALKFTRGEGVISVFVGREGDDAVLRIADQGIGIPADMLPKI
                     FDLFVQAHHTIDRTRGGLGIGLTLVRRLAELHGGTVHAASEGDGHGSTFTVRMPTVAA
                     PKVADEAVAVTSNGNASRRAFESRHVLLVDDNHDSREMYRAVLRAHGHDVCEAPDGER
                     ALALLQQRANDHPLDIAFIDIGLPGGMDGYELARRIRSLPTGRYVRLVALTGYGFPED
                     RQQSKQAGFARHLVKPVEPEALQRELNAVTR"
     gene            complement(3568..4872)
                     /locus_tag="VEW08_07685"
     CDS             complement(3568..4872)
                     /locus_tag="VEW08_07685"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HYJ40654.1"
                     /translation="MRIRNGATLALGLVAALALNACGSSDEDGGEDPKIRLLNLSAGY
                     TALDLMTNLDSDDDDDDATQATAVALESVSDYTTLDPDDYTVKVRRTGSGSVLRSFAG
                     EELVEDTINTYVAYGEVGNFGALRIDDTLDEADAGDSKLSVANLSSAGSLDVYLTEAN
                     TDLDDTTPVLSNIGAALSLVIADSGSFRLRVTAVGDTADVRLDVPNFTLADRGVATLI
                     LTSTQGGMLANAIFLPQEGQPTKYTNTKARLRGAVGLASGANASIQVGGQSVLSAATA
                     GVIGSRYTLLDAGSVPVSLSVNGAAVPVSNLNLTAGADYTLLVWSNTVGPQTSLVNDD
                     NRLPSGGGTKLRLLNGMSTLAAPITLSVDFSPVIEGTLTGQVSDEIEISSGTDRQFDV
                     SNTSTAQNVLTRSAITLQPSSVYTFFMTDNGATPIGVLRRDR"
     gene            complement(4894..>5281)
                     /locus_tag="VEW08_07690"
     CDS             complement(4894..>5281)
                     /locus_tag="VEW08_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HYJ40655.1"
                     /translation="RGFRDGSLGPRDSLGNPYGGDAGVSAQFEAIVPLPAKFASAARV
                     SLFYDIGQSFYLGDTEFRNKRGDRTDYRFDLGELRSSAGIAVQWLSPMGLFRFSWAVP
                     LRYQGETRREFGDELEPFQFSVGTAF"
BASE COUNT         1017 a         1726 c         1629 g          909 t
ORIGIN      
        1 aacggcgctc agcaattcgc cgagccagga gtactcatga cattcgagga aaacgccgcg
       61 gagctcgccc aggatgtgcg ctcgctcggc tacaacctcg attcactggt ggcgcaaaag
      121 aagctcatcg tcgactacgt ccatgtcgat cgcagcgaaa tcgaggaaac cggcgaatat
      181 gacctcgagg gtctgttcgt gcggctcgaa catgccatca aggaaatcgg cgccaggcgc
      241 gtgatgctcg acaccgtcga gacgcttttc ggcggcctga aggacaccgg catcctgcgc
      301 gccgagcttc ggcggctgtt tcgctggatg cgcgatcgcg gcatcaccgc cgtcgtcacg
      361 gccgaacgcg gcgagcagat gttcacgcgc caggggctcg aggaatacat ctccgacgcc
      421 gtgatctcgc tcgatcaccg cgtgcacgaa cagatctcca ctcgccgcat ccgcgtcgtg
      481 aaataccgtg gctccacgca cggcacgaat gaatatccgt ttctcatcga tcgggacggc
      541 atcaccgtgc tgcccgtcac ctcgctgctg ctcgagcacg aagcgccgaa cgagcgcatt
      601 tcgaccggcc tcaagtcgct cgacgacatg ctgggcggca agggctactt ccgcgccagc
      661 acggtactgc tctccggcac ggccggcacg ggcaagacca cgctcgccgc gcagttcatc
      721 gaggccgcct gcgcgcgtgg cgagaagtgc atgtacttcc tcttcgagga gtcgccctcg
      781 cagttgatac gcaacatgcg ttcggccggc atcgacctgc aaaagtggct ggacaagggc
      841 ttgctcaagt tccatgccga ccgccccaat cgtcacggcc tcgagaccca cctgctggag
      901 atgcaccatg ccgttgaagt gttcgacccc gatgtggtgg tgctcgaccc gatcaccaat
      961 ctcatggccg ttggcacgca atccgatgtg cgcgccatgc tcacgcgagt catcgactac
     1021 ttcaagacgc gcggcgtcac cgcggtgttc accagcctca cctcttcgac gcaaagcctc
     1081 gaggcgaccg agtccatgat ctcctcgctg atggacacct ggatcctcgt ggccgtcgcg
     1141 gaagaagcgc gcaagcgtaa ccgttggatc tacgtgctca agtcacgcgg catgccgcat
     1201 tccaacgatg tacgcgcgtt cgagatcacc gaccacggcg ttgccatcac gcctctcggc
     1261 aggaacggct tggccgggaa acccaagccc gcaaggaaac ggaaatgaat gaaaattacg
     1321 cgctgaaact gtacgtcgcc ggccagacgc cgaaggccgt gcgcgcgttc tccaatcttc
     1381 gcaagatctg cgaggagcac ctgcaagggc gctacagcat cgaagtcgtc gacctcatcg
     1441 agaacccggc actgggtcga ggcgaccaga tcctggcatt gccgacactg gtgcgccgac
     1501 tgccgacccc aataaagaag atcatcggcg acctgtccaa cactgaacgt gtgctcgtgg
     1561 gtctcgatct ccgccctcgc cggcccagca cgtgaatgag accccactcc acaccctggc
     1621 cgacctggcc gatcaggcag aaaggcagcc atacgtgctg cgtctgtacg tgaccggcat
     1681 gaccccgcga tccacccggg ccatcagcgc cgtgcgcgcg atttgcgagg aattcctcgc
     1741 gggccgcttc gatctcaaga tcatcgacgt gtaccagcaa cccgcgttga tccgggacga
     1801 acagatattc gcaaccccca ctctcgtcag gaagggcccc gagcctgagc gccggatgat
     1861 cggcgacatg tcgaaccgcg cacgcctcct cgcagggctc ggccttctag aagtgacccc
     1921 tgcatgaaca tcgaagacct cgacatcgaa gaacttcgcg cccgcctggt agaggcggag
     1981 ggcaacctgc aagccatcat cagcggcggc atcgacgccc tgttgctggc cgacgaaaac
     2041 ggcgagcggc gcgtatttac gctgcgcagt gccgacgcac cgtatcgcgc gctggtcgag
     2101 cggatgcagg agggcgcggc gacgctctcg atcggcggcg acatcgtgta ttgcaaccag
     2161 cgtttcgccg acctggtgca gatgccgctc gagcaggtct tcggcgcgcc gatcggaaaa
     2221 tttctgccgg cggccgaggg cgaaatcctg cgcgccatgc tcgaacatgg ttcggggcgt
     2281 taccagacgc gcctggtgct gggcggccag gaatcgaccg aggtgcaggt gacgttctcg
     2341 agcatcgtgc tcgacgacgt cgaacatcgc acgctgatcc tctccgacat cagctcgctg
     2401 gcacgagcgc agcgcgagaa ccgttcgaag gacgagtttc tcgccatgct ggcgcatgag
     2461 ctgcgcaacc cgctcggcgc catccagggc gcggtgcacg cgctcgccct gatgaatcag
     2521 accgagccca tggcgttgcg cgccacggga atcatcaagc ggcaggtagt gcacatggct
     2581 cggctggtgg atgacttgct cgatgtcggc cgcgcggtca ccggaaagat cgtgctgaag
     2641 cgcgtcccgg tgaatctcgc ggaggctgtc aaggcttcga tctctgccct tgcctcgggc
     2701 aatgccagcc accagggccg catcgacctc gtgtccgaac ccgtgtgggt gagtggcgac
     2761 ccggtgaggc tcgagcagat cgtcggcaat ctcgtgtcca acgcgctcaa gttcacgcgc
     2821 ggcgaaggcg tgatcagcgt gttcgtcggg cgcgaaggtg acgacgccgt gctgcgtatt
     2881 gccgaccagg gcatcggcat tcccgccgac atgttgccga agatcttcga cctgttcgtg
     2941 caggctcacc acaccatcga tcgcacgcgc ggcggtctgg gaatcgggct cacgctggta
     3001 cggcggctgg cggaacttca cggtggtacc gtacacgccg ccagcgaagg cgacggccat
     3061 ggctccacgt tcacggtgcg catgccgacg gttgccgcgc cgaaggtggc ggacgaagct
     3121 gtggcggtga cttcgaacgg caatgccagc aggcgcgcct tcgaatcccg ccacgtgctg
     3181 ctcgtggacg acaaccacga ttcgcgcgag atgtaccgcg ccgtgctgcg cgcgcacggc
     3241 cacgatgtgt gcgaagcgcc agatggcgaa cgcgcgctgg cgttgctgca gcagcgcgcg
     3301 aacgatcacc cgctcgacat cgcattcatc gacatcggtc tgcccggtgg catggatggt
     3361 tatgagctcg cgcgccggat ccgcagcctg cccacgggtc ggtatgtgcg gctcgttgcg
     3421 ctcaccggct atggttttcc cgaggaccgc cagcaatcga aacaggccgg gttcgcgcgg
     3481 cacctcgtga agcccgtcga acccgaggcg ttgcagcgcg agctgaatgc cgtcacccgc
     3541 tagatgggga tactccccat ttccaattta gcggtctcgg cgcaacaccc cgatgggcgt
     3601 cgccccgttg tcggtcatga aaaatgtgta cacggagctg ggctgcaacg tgatagcgct
     3661 gcgcgtcagc acattctgcg ctgtcgaggt gttcgacacg tcgaactgcc ggtcggtgcc
     3721 cgaggagatc tcgatctcgt ccgacacctg gcccgtcaac gtgccttcga tgacgggtga
     3781 aaaatccacc gacagggtaa tcggcgcggc cagtgtcgac atgccgttca acaaacgcag
     3841 cttggtgccg ccgcccgaag gcagccggtt gtcgtcattg accaggctgg tctgcggacc
     3901 aaccgtattg ctccacacca acaacgtgta gtcggcaccc gccgtgagat tgagattcga
     3961 caccggcacg gcagcgccat tcaccgaaag cgacaccggc acagacccgg catcgagcaa
     4021 cgtgtagcgg ctgccgatca cgcccgcggt ggcggcactc agcaccgatt ggccacccac
     4081 ctggatcgaa gcgttcgcgc cactggcaag acccaccgcg ccacgcagtc gtgccttggt
     4141 gttggtgtac ttggtgggct ggccttcttg cggcaggaag atggcgttcg caagcatgcc
     4201 gccctgtgtc gaggtaagaa tgagcgtggc aacgccgcga tcggccagcg tgaagttcgg
     4261 gacatcgaga cgcacatcgg cagtgtcgcc aaccgcggtc acgcgcaggc gaaagctgcc
     4321 actgtcggcg atgaccaggg acagcgccgc accgatgttc gacagcacgg gcgtagtgtc
     4381 atcgaggtcc gtgttcgctt cagtcaggta gacgtcgagc gaacccgccg aggacaagtt
     4441 ggccacgctg agcttgctat cgccggcgtc cgcctcgtcg agcgtgtcgt cgatgcgcag
     4501 cgcgccgaag ttgcccactt cgccataagc gacataagta ttgatggtgt cttcgaccag
     4561 ctcttcaccc gcgaacgagc gcagcacgct gcccgaaccg gtgcgccgca cctttaccgt
     4621 gtagtcatcg ggatcgagcg tggtgtagtc gctaacggat tcgagcgcca cagctgtcgc
     4681 ctgggtcgcg tcgtcatcgt cgtcatccga atccagattg gtcatgaggt cgagcgcggt
     4741 ataacccgcg ctcaggttga gcaagcggat cttcgggtct tcaccaccat cctcatcact
     4801 gctaccgcaa gcgttgagcg cgagtgccgc cacgagaccc aaggccaaag ttgctccgtt
     4861 acgaattctc attccattca ctccatctat gcgtcagaac gcggtgccga ctgaaaactg
     4921 aaaaggctcg agctcgtcgc cgaactcgcg gcgggtctcg ccctggtaac gcagtggtac
     4981 tgcccaggaa aatcggaaca ggcccatggg cgacagccac tgcacggcga tgccggccga
     5041 actgcgcagc tctccgagat cgaagcgata gtcggtgcgg tcgccgcgct tgttgcggaa
     5101 ttcggtgtcg cccaggtaga acgactgccc aatgtcgtag aacaggctca cgcgcgccgc
     5161 gcttgcgaac ttcgccggca gcggcacgat ggcttcgaac tgcgccgaca cacccgcatc
     5221 accgccgtaa ggattgccca acgaatcgcg cggcccgagc gagccgtcgc ggaatccgcg
     5281 c
//
LOCUS       DASQJS010000185        10619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2615545, whole genome shotgun sequence.
ACCESSION   DASQJS010000185 DASQJS010000000
VERSION     DASQJS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10619
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2615545"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1246)
                     /locus_tag="VEW08_07695"
     CDS             complement(236..1246)
                     /locus_tag="VEW08_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ40656.1"
                     /translation="MTGFPLDPSFRPRRWLRGRHFQTILSTLPPRRARVERRAIPLRA
                     ASEELLLECGDGTVLQAFHASPARRGRDPGKRLAVLLHGWEGSADSTYVLSASQTMFD
                     LGFEVVRLNLRDHGATHHLNRDLFHSCRLPEVVGAVRSLASRFAGMPMVLGGFSLGGN
                     FMLRVAADREARDLPLQRVVAVSPVLDPAVTMTTLERGWPLYHAYFVRKWSRSLAKKQ
                     QAWPDLYDFEELLRTRSLREMTRQLVQRHTEYPSMDAYLAGYAITGDRLTTLAAPATV
                     LTSLDDPIIEAGDLARLARTPRLDIVTTAHGGHCGFLENLSGTNWTDAQLVKLLSRNP
                     DS"
     gene            complement(1243..2352)
                     /locus_tag="VEW08_07700"
     CDS             complement(1243..2352)
                     /locus_tag="VEW08_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ40657.1"
                     /translation="MSSKRALVVDDSKSARAFLSRILERHEIMVDAAESAEAAIDYLT
                     RNRPDVIFMDHMMPGMDGFQAVQSIKNNPRTAAIPILMYTSQEGDLYLGQARALGAEG
                     VLPKQIKQADVTKMLFQLQLVSDRRTRERSVFTAADQAPNLVPANESAIEVDQPAVAS
                     SAVTETATVAADQQFALLLPKMTLEIRAALDHSLQKEIAALRGFIGTTLDAHAERLQG
                     DLATLLPATRTPDLDLPTVMPERRSWGAIFGWSLAVIAAAGAAFMTWLWWNQGGEMAA
                     LRTDLSAAYAELEALRARPAVVSPAPLPVEPAAEPAADPSVVLPDEDAPDAAAVATPT
                     VELVPTGTAATPVVPPATMPATTAPAAAPPASQAQ"
     gene            complement(2489..3709)
                     /locus_tag="VEW08_07705"
     CDS             complement(2489..3709)
                     /locus_tag="VEW08_07705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYJ40658.1"
                     /translation="MRVLFVSDVYFPRVNGVSTSIRTFRSDLAQLGVETMLVAPSYPG
                     APDDADSSIIRVPSGAVPRDPEDRRFLSGPLRRVLDATLAARVDLVHIHTPFIAHYAA
                     ARFARTHGLPVVATYHTFFEDYLHHYVPILPRGIGRWIARRFTLSQCDDVAELISPSA
                     PMRDALLAYGVITPIEVLPTGLPAESFLQGDGARFRKSFDLPPDRPLLLYVGRVAFEK
                     NIDFLLRMFARLRARRPDALFVIAGEGPALPHLTRLARELGVESHVRFIGYLDRATEL
                     PDCYAAGNAFVFASRTETQGLVLLEAMAQGTPVVSIAELGTRSILTADCGAYVVPEDE
                     EVFATAVAEALQLETKSPRRAQLRAHAESWASLAMARRLLAFYERVRASSAQRLSNDE
                     RGPIGTPLGVERSS"
     gene            complement(3709..4242)
                     /locus_tag="VEW08_07710"
     CDS             complement(3709..4242)
                     /locus_tag="VEW08_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYJ40659.1"
                     /translation="MDSHGSLLARFDAAEYRLCRRLNRGVANAGIRRFFKTASRLGDG
                     VIWYVLMVALPFFYGAEGLEVALIMLATSAVGLAIYKFLKRTFVRERPFIRHAGISLA
                     GAPLDRYSFPSGHTLHAVAFTWQGCAAFPELSVVLVPLALAIAASRVVLGLHYPTDVL
                     VGALLGATTGSIGASFL"
     gene            4409..6490
                     /locus_tag="VEW08_07715"
     CDS             4409..6490
                     /locus_tag="VEW08_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40660.1"
                     /translation="MNWCRMARLGKSGAALLLLSALAPAVHAVDTIVLEVGEMSVDGI
                     PVEAAAARLDLLSDKQTRVTLSARAVSLPDPAGKLTDLTLVCNSPVIAEPRFGCDAGQ
                     LTGRGGPTGSIDAAMTAMMNTDTGVTTFGGRGLKLAGTTASFDGTLDEKGWQVKGRTG
                     TTTVEALRKFAAPWFELPKDITGDGKVVVEGNAVDSGAGTVLDTTLNLDAVTLTNEAS
                     TIVTDSLSAVVRLRARLNDADTALELNLTGKQGQVLVNPVLFDFGKNPMTFESRANLK
                     GDVMAVESLRLAQKDLIELTGKGSVNLAGETPLVSGDFQLAKLQFPAAYTSYLQITLA
                     TTRVLSDLKMGGSLNGEFTVRDNGITAMRISPKDLQVNDEKDSLHLTKVNGDVYWRPE
                     GTANAPASKLSWASGGAYGLSGGAASFDFLAQGAGFHITHPTKLPIFDGALAIDTLSL
                     GNLDGENMEVAFKGNVEPISMPLLTKAFGWPEFSGTLAASIPGVTLKDNVLTFQGNVE
                     SQVFGGRIVGSNIRMQDPLGRFPQFFADVRARDLDLGLLTQTFEVGSITGKLEVDVLG
                     LELFAWSPTAFSARLATPKGDKSRHRISAKAVSSLSNVGGGGGGVVQALQSGVLKFFD
                     EYNYDKLGITCTLVGDVCEMSGIEPAGVGYYIVKGAGLPRIDIIGSQGRVNWNQLLSS
                     IPTDFSTATVD"
     gene            6632..7261
                     /locus_tag="VEW08_07720"
     CDS             6632..7261
                     /locus_tag="VEW08_07720"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HYJ40661.1"
                     /translation="MRRVLPLKIALASLLVAACVTINVYFPAAAADKAADQVINDITG
                     GGARSGTTTPPQSNMVPASSRAEPNFLVAAFGNALYALVPTANAQDADAALNVSSPAV
                     TRIKQSMGARFGELEKFFASGAIGLTRDGMVDVRDLNAVALPDRATVKRLVSEDNADR
                     AQLYAEIAKANNHPEWEADIKKSFAKRWIATGAKPGWYYQGDDGSWKQK"
     gene            7271..8152
                     /locus_tag="VEW08_07725"
     CDS             7271..8152
                     /locus_tag="VEW08_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ40662.1"
                     /translation="MTTTGPVPHRGAGLSFFRRRAVLFGIATIALVVIAAVWLVIALQ
                     RIVTFPGAHPSVQQPAALAAAGGEAVWFDIDGARVEAWFLPAQSRKLPVPLMIHAHGN
                     GELIDIQTQSVQAIRAAGFAVLLLEYPGYGRSGGNPSEETVTDTWVAAYDWAKSDPRV
                     DAARIVGYGRSLGGGAVAQLAARRPIAALVLESSFTSIGELVRAEGIPGWLVVNEFDT
                     RAVLAKYSGPVLILHGTHDQTFPVEHAHSLRAASPHATLHLQECGHNDCPPQWELVLG
                     FLAQNGVISEPVSGDSR"
     gene            8149..8385
                     /locus_tag="VEW08_07730"
     CDS             8149..8385
                     /locus_tag="VEW08_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40663.1"
                     /translation="MTTSTIFGLRSGLAVVAATLLLTGCGLAETTAVAATQAEAAAEQ
                     AKEGKKMEEKVQRDIAAADQAAADARAKAEAANE"
     gene            complement(8876..9694)
                     /locus_tag="VEW08_07735"
     CDS             complement(8876..9694)
                     /locus_tag="VEW08_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ40664.1"
                     /translation="MNDVAAQEPAADSVRSLRQARWTGLKTIIIREYGRIIRIWAQTI
                     VPSAVTSALYFVIFGSLIGQRVGLMDGVPYMQYIAPGLIMMAVITNSYGNVVSSFFGA
                     KFGKHVEEMLVAPLPNWIIILGYVGGGLVRGFLVGGAVTAVSLIFTQLTVHHAFVILA
                     AVVLTSVVFSLGGFINAQFAKNFDQVNWIPTFVLTPLTYFGGVFYSISLLPEWARHLS
                     YANPILHMVNAFRYGFLGTSDVNVFVAFGIMLLSAVVLFAVALIFMNRGTGMRE"
     gene            complement(9691..>10619)
                     /locus_tag="VEW08_07740"
     CDS             complement(9691..>10619)
                     /locus_tag="VEW08_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ40665.1"
                     /translation="LSIRGLTKTYKNGVQALKGVDLDVETGDFFALLGPNGAGKTTLI
                     GILTSLVNKSAGTVSVFGHDLDRELEAAKACIGIVPQEINFNQFETPQTIMVNQAGFY
                     GIERPLARERTEKYLSLLQLSDKRNKIARGLSGGMKRRLMIARALVHEPRLLILDEPT
                     AGVDIEIRRSMWDFLREINARGTTIILTTHYLEEAETLCRNIAIINGGLIAERDRMST
                     LLRKLQAETFVLNLREAVRTVPILPGYDFELIDEHTLEVEVSKEQSLNEVFSRLTEQG
                     IGVLSMRNKVNRLEELFMKLVERKPGVAASSS"
BASE COUNT         2022 a         3293 c         3484 g         1820 t
ORIGIN      
        1 cggtgacgcc gccgggaacg ctgggtgggg tggtatcggt gaccggtggc ggcggcgggg
       61 gtggatcttc gctcccgggg ccgttgctgc agcctccgag caatacggca agcgcctgaa
      121 ttcccaggct cgctgcgagc cgcggcgtaa tccgcaccat acgagagacc tccgaaattc
      181 ccgggctttg cgcccgtggg gttgttagca gctcagcaca ctcgtgccgg gccgcctagc
      241 tgtccgggtt acgcgacagc aatttgacca actgggcatc agtccagttg gtgccgctca
      301 ggttctcgag aaagccgcaa tgcccgccat gtgccgtggt gacgatgtcc aggcgcggcg
      361 tgcgcgccag ccgcgcgaga tccccggctt cgatgatggg atcgtcgagc gaggtcagca
      421 cagtagcggg cgccgcaaga gttgtcagac gatcacccgt gatcgcatag ccggcaagat
      481 aggcgtccat gctcgggtac tcggtatgcc tctgcaccag ctggcgggtc atctcgcgca
      541 ggctgcgggt gcgcagcagt tcttcgaagt cgtagaggtc gggccaggcc tgctgcttct
      601 ttgccaggga ccggctccac ttgcgcacaa agtaggcgtg atacagcggc cagccacgct
      661 cgagcgtggt catcgtcact gcagggtcga gaaccggcga caccgcgacc acacgttgca
      721 gcggcaggtc gcgcgcctcg cgatccgcgg ccacgcgcag catgaaattg ccacccagtg
      781 aaaagccgcc cagcaccatg ggcatgcccg cgaaccggct cgcgagcgag cgtaccgcac
      841 cgaccacttc gggcaggcgg caggaatgga acaggtcacg gttcaaatgg tgcgtggcgc
      901 cgtgatcgcg caggttgagc cgcacgacct cgaagccgag atcgaacatg gtctgcgaag
      961 cggacaggac gtacgtggaa tcggcgcttc cctcccaccc atgcaacaac accgcgagac
     1021 gtttgccggg atcgcggccg cgcctggctg ggctggcgtg aaacgcctgc aggaccgtgc
     1081 cgtcaccaca ttcgagcagc agctcttcgc tcgccgcgcg caaggggatt gcgcgccgct
     1141 ccacgcgcgc gcggcgcggc ggcagggtcg agagaatggt ctggaaatgc cggccgcgaa
     1201 gccagcgacg gggtcgaaac gaagggtcga gggggaagcc ggtcactgcg cctgactggc
     1261 tggcggcgct gcagcgggag ccgtggtcgc gggcatcgtg gcaggcggga ccaccggtgt
     1321 ggcggctgtg cccgtgggca ccagttctac cgttggcgtg gccacggccg cggcatccgg
     1381 cgcatcttcg tcaggcaata cgacgctggg atcagccgcg ggttcggcgg caggttcgac
     1441 cggcaatggc gccgggctaa ctaccgcagg cctggcgcgc aacgcttcga gctcggcgta
     1501 ggcggcggac agatcggtgc gcagcgcggc catttcgcca ccctggttcc accacagcca
     1561 ggtcatgaac gccgcgcctg ccgcggcgat gacggccagc gaccagccga agatggcgcc
     1621 ccacgaccgc cgctcaggca tgacggtggg caggtcgagg tccggtgtcc gcgttgccgg
     1681 aagcaacgtt gcaaggtcgc cttgcaggcg ctcggcatgc gcatcgagcg tggtgccgat
     1741 gaagccacgc agcgcggcga tttccttctg cagggagtga tcgagggcgg cccggatttc
     1801 gagagtcatc tttggcagca gcagcgcgaa ttgctgatct gcggccacgg tggccgtttc
     1861 ggtgaccgca gaagaagcga ccgccggctg atccacctcg attgctgatt cgttggccgg
     1921 gacgagattc ggcgcctgat ccgctgcggt gaaaaccgag cgctcacgcg tgcggcgatc
     1981 cgagaccaac tgcagctgga acagcatctt ggtgacgtcg gcctgcttga tctgcttggg
     2041 cagcacacct tcggcgccga gcgcgcgcgc ctgccccagg tacagatcgc cttcctgcga
     2101 ggtgtacatg aggatgggaa tggccgcggt gcgcggattg ttcttgattg attgcacggc
     2161 ctggaagccg tccatgcccg gcatcatgtg atccatgaag atcacgtcgg ggcggttgcg
     2221 cgtgaggtag tcgatggcgg cttccgcgga ttcggccgcg tccaccatga tttcgtggcg
     2281 ttcgagaatg cgcgacagga atgcgcgtgc ggacttcgaa tcgtccacaa ccagagctcg
     2341 tttggaagac atcagctctc ctctccttca aacacatcaa ccggtttgag gaaagtctag
     2401 cgtcaagaga cgcgcagtgc gcgcgtgatc cggatttttt tcagggggag tggagaagtt
     2461 ttcgaaatct gtaaattttt tcgacttctc aggagctgcg ctccactcct aacggggttc
     2521 cgatgggacc ccgttcatca tttgaaagac gctgagccga actagctcga actcgttcgt
     2581 agaacgccag cagccggcgc gccatcgcca gcgaagccca cgactcggca tgcgcccgca
     2641 attgcgcgcg gcgcggcgat ttcgtctcga gttgcaacgc ttcggcgacg gcggtcgcaa
     2701 agacctcttc gtcctcgggc accacgtatg cgccgcaatc ggcggtgagg atggagcgcg
     2761 tacccagctc ggcgatggat acgaccggag tgccctgcgc catggcctcc agaagcacca
     2821 gcccctgggt ctcggtgcgc gaggcgaaaa cgaacgcgtt gcccgccgcg taacaatccg
     2881 gaagctcggt ggcgcgatcc aggtagccga tgaaacgcac atgagattcc acgccgagct
     2941 cgcgtgccag cctggtgaga tgcggcaatg ccgggccttc tcccgctatg acgaacaatg
     3001 catccggccg gcgcgcgcgc aggcgcgcga acatgcgcag cagaaagtcg atgttctttt
     3061 cgaacgctac gcgccccacg tacagcagca gcggacggtc gggcggcaga tcgaacgact
     3121 tgcggaatcg cgcgccgtcg ccctgcaaga aactttccgc gggcagcccc gtcggcagca
     3181 cttcgatggg agtgatgacg ccataagcca gcaaggcgtc acgcatcggt gcgctgggcg
     3241 agatcagctc ggcgacatcg tcgcactgcg agagcgtgaa tcgccgtgca atccagcggc
     3301 cgatgcctcg cggcaggatc ggcacgtagt gatgcaggta gtcctcgaag aacgtgtgat
     3361 acgtggcgac cacgggcagg ccgtgcgtgc gcgcgaaccg cgcagcggca tagtgcgcga
     3421 tgaagggcgt atggatgtgc accaggtcga ccctggccgc gagggtggcg tcgaggaccc
     3481 gccgcagcgg accgctcaga aagcggcgat cttccggatc acgcggtacc gcgcccgagg
     3541 ggacacgaat gatcgatgag tccgcatcgt cgggcgcgcc ggggtacgac ggtgcgacca
     3601 gcatcgtctc cacgccgagc tgcgccaggt cggagcgaaa ggtgcggatc gaagtggaga
     3661 cgccgttgac ccgggggaaa tagacgtcgg aaacgaacag tacccgcatc agaggaacga
     3721 agcgccaatc gagcccgtgg tcgcgcctaa caaggcaccg accagcacgt ccgtcgggta
     3781 gtgcagccca agcaccactc gcgacgcggc aattgccagc gcgagcggaa ccagcaccac
     3841 cgaaagctcg ggaaatgccg cgcagccctg ccacgtgaac gccacggcat gcagcgtgtg
     3901 gccggatggg aagctgtatc gatccagcgg cgcacccgcc agcgagatgc ccgcatggcg
     3961 gatgaagggg cgctcgcgca caaaggtgcg cttgaggaat ttgtagatgg ccaggccaac
     4021 ggcgctggtc gccagcatga tcagcgcgac ttcgaggcct tcagctccat agaagaaggg
     4081 cagcgcgacc atgaggacgt accagatgac gccgtcaccg agccggctgg cggtcttgaa
     4141 gaaccgtcgg atgcccgcgt tcgcaacgcc gcggttgagc ctgcggcaga gtcggtactc
     4201 cgccgcgtcg aatcgagcca gtagtgagcc atggctatcc atggggtgca cagtctggtg
     4261 ggaggacaag gcggcggcat gacgaactca tgaaggattt attgctaagt atctgaaatc
     4321 ccttagcagg gcgcctgttt gggctgttcg aatgttgagc agttgcgtcc gacagcccga
     4381 tttcgggtgg gcgttacaat cgccgcccat gaattggtgc cggatggcgc gcctgggaaa
     4441 atccggcgcc gctctactct tattgtcagc actcgcgcct gcggttcacg cagtcgacac
     4501 catcgtgctc gaggtcggcg agatgagcgt ggacggcatt ccagtggagg ccgccgctgc
     4561 gcgcctggac ctgctctccg acaaacaaac ccgcgtgaca ctcagcgcgc gcgcggtttc
     4621 gttgcccgat cccgcgggca agcttacgga cctcacgctc gtgtgcaatt cgccggtgat
     4681 cgccgagccg cgtttcggct gcgacgccgg gcagctgact ggtcgcggcg gtccgactgg
     4741 ctccatcgat gcggccatga cggcgatgat gaacaccgac acgggcgtga ccaccttcgg
     4801 cggacgcggc ctgaaactgg cggggacgac ggcgtcgttc gacggaactc tcgatgaaaa
     4861 aggctggcag gtgaaaggcc gcaccggcac gacgacggtt gaagctctgc gcaagttcgc
     4921 ggcgccgtgg ttcgagctgc cgaaggacat cacgggcgac ggaaaggttg tggtcgaagg
     4981 caacgcggtc gattccggcg ccggcacggt gctggacacc acgctgaatc tcgatgcggt
     5041 gaccctgacc aacgaggcga gcaccatcgt taccgacagt ctctccgccg tcgtgcgcct
     5101 gcgcgcgcgc ctcaatgacg ccgacaccgc gctcgagctc aacctgaccg gaaaacaagg
     5161 acaggtgctg gtcaatccgg tgttgtttga tttcggcaag aatccgatga ctttcgagtc
     5221 gcgcgccaac ctgaaaggcg acgtgatggc ggtcgagtcg ctgcggctag cccagaagga
     5281 cctcatcgaa ctcactggca agggcagcgt gaacctggcc ggcgaaactc cgctggtcag
     5341 cggtgacttc cagctcgcaa aactccagtt ccctgccgct tacacgagtt acctgcagat
     5401 cacgctggcc accactcgcg tgctcagcga tctcaagatg ggcggtagtt tgaatggcga
     5461 gttcacggtt cgtgacaacg gcatcacggc gatgcggatt tctccgaaag acctgcaggt
     5521 caatgacgag aaggactccc tgcacctgac gaaggtgaac ggggacgtgt actggcgacc
     5581 cgaaggtacc gccaatgcgc ccgcctcgaa gctttcctgg gcgagtggcg gcgcgtacgg
     5641 attgtcgggt ggcgcggcct cgttcgactt cctggcgcag ggcgcgggtt tccacatcac
     5701 gcaccccacc aaactaccca tattcgacgg cgcgctcgcc atcgacacgc tgtcactcgg
     5761 caacctcgac ggcgaaaaca tggaagtggc attcaaggga aacgtcgagc cgatcagcat
     5821 gccgctgctg accaaggcgt tcggctggcc ggagttttcc ggaacgctcg cggccagcat
     5881 tcccggcgtc acgctcaagg acaatgtgct cacgtttcag ggtaacgttg aatcgcaggt
     5941 gttcggcggg cgtattgtcg ggagcaacat acgcatgcag gatccgctcg gccgattccc
     6001 gcaattcttc gccgacgtgc gcgcgcgtga cctcgacctg ggcctgctga cgcaaacgtt
     6061 cgaagtgggc agcatcaccg gaaagctcga ggtcgacgtg ctcggcctcg agttgtttgc
     6121 atggtcgccg accgctttca gtgcccggct tgccacgcca aaaggcgaca aatcgcggca
     6181 tcgcatcagc gccaaggcgg tgagcagcct gtcgaacgtg ggcggcggcg gtggcggcgt
     6241 ggtgcaggcg ctgcagtccg gcgtgctgaa attcttcgac gaatacaact acgacaagct
     6301 gggcatcacc tgcacgctgg tcggcgacgt gtgtgaaatg tcgggcatcg agcccgccgg
     6361 cgttggttac tacatcgtca agggagccgg tttgccgcgc atcgatatca tcggaagcca
     6421 ggggcgcgtg aactggaatc agctgctgtc gtcgattccc acggacttca gcaccgccac
     6481 ggtggattga gccagatcgt cgtgtggact cggcgcccgt tcgggccaat aatcccgcgc
     6541 acatttgacg taaggcacgt tcagggcgca ttcatcggcc gcgcccgatc atgtccgcgt
     6601 ccaggcttga gcttggaata aggagttatc aatgcgacgc gttttgccgc tgaaaattgc
     6661 actggcgagc ctcttggtcg ccgcgtgcgt cactatcaac gtttactttc cggccgctgc
     6721 cgccgacaaa gctgccgacc aggtgatcaa cgacatcacc ggcgggggcg caaggagcgg
     6781 caccaccact ccgccacagt ccaacatggt gcctgcatcg tcgcgcgcag agcctaactt
     6841 cctcgtggcc gcgttcggca acgccctgta cgcgctggtc cccacggcca atgcgcagga
     6901 tgccgacgcc gcactgaacg tgagttcgcc ggcggtcacg cgcatcaagc agtccatggg
     6961 cgcaaggttc ggtgaactcg agaagttctt cgcgagcggc gccatcggcc tcaccaggga
     7021 tggcatggtc gacgtgcgcg atctcaacgc ggtggcgttg ccggaccggg cgaccgtcaa
     7081 gcgcctggtg tccgaagaca acgccgaccg tgcgcagctc tatgccgaaa ttgccaaggc
     7141 caacaaccac ccggagtggg aggccgacat caagaagagc tttgcgaaac gctggattgc
     7201 caccggcgcc aagcccggct ggtactacca gggtgacgac ggttcctgga aacagaagta
     7261 atctagttag atgacgacga cagggcccgt tccgcatcgc ggggcgggcc tttctttttt
     7321 ccggcggcgt gcggtcctgt tcggtatcgc caccatcgcg ctggtggtga tcgcggcagt
     7381 ctggctggtc atcgcgctgc aaagaatcgt cacgtttccc ggcgcgcatc cctcggtgca
     7441 gcagcccgcc gcgctcgcgg ccgctggcgg tgaggctgtc tggttcgata tcgacggcgc
     7501 gcgcgtggag gcatggttcc ttccggcgca aagcaggaag ctgcccgtgc cgttgatgat
     7561 tcatgcgcat ggcaacggcg aactcatcga catccagacc caatccgtac aggcgatccg
     7621 tgcggccggc tttgccgtgc tgctgttgga atacccgggc tacgggcgtt cggggggcaa
     7681 cccctcggaa gagaccgtca ccgacacctg ggtggccgcc tacgactggg cgaagagcga
     7741 cccgcgcgtc gacgccgcgc gtatcgtggg ttatggacgc tcgctgggcg gaggcgccgt
     7801 cgcgcaactc gccgcacggc gaccgatagc ggccctggtg ctggaatcca gtttcaccag
     7861 catcggcgag ctggtgcgcg cggagggcat tccgggctgg ctcgtggtga atgagttcga
     7921 cacgcgtgcg gtcctggcga agtattctgg ccccgtgctc attctgcacg gcactcacga
     7981 tcaaactttt cccgtcgagc acgctcattc cctgcgtgcg gcatcgccac acgcgacgct
     8041 gcacctgcag gagtgcggcc acaacgattg tccgccgcaa tgggagctgg tactcggttt
     8101 cctcgcgcag aacggcgtaa taagcgaacc agtatccgga gactcacgat gactacttca
     8161 acgattttcg gcttgcgctc ggggctggcg gttgtcgcgg ccaccttgct gctcaccggc
     8221 tgtggcctcg cggagacaac cgcggtcgcc gccacccagg ccgaggcggc tgcggagcag
     8281 gccaaggaag gcaagaagat ggaagagaaa gtgcagcgcg atatcgccgc ggcagatcaa
     8341 gccgcagccg acgcgcgcgc gaaggctgag gcagcaaacg aatagaaatg gggacattcc
     8401 gccagtcctg acggaccggc cctggcgtgc gcgcctgaac tgtcagtact tgccacgcat
     8461 tcgctacacg gagacccgtc cccatttccc agcggcggtg gtaagagaac aggtgcacgg
     8521 gtgtaggtct tcccacagcg tggggttaca tgcctctttt cctggcgaac gaagtcgcga
     8581 cgcgtcggtg cacgatcgag cgacgggcgg ccctgcatgc gatgagcgag cgggccggga
     8641 tactttgctt cgctaggcac gaagccgttc cctgcgacat caggactctt cgcctcctta
     8701 gctccaacca cccacaagat gtcatcgtgt gtgtcggcgg cccgcaaact ggcggcaagc
     8761 aactaacgaa gggcccggcg aaaagcgagt ctcatcgcat gcagggccgc ccgtcgctcg
     8821 accatccacc aacgagccgc gtcccctctt gccagcaaac gaggaatgtc cccctttact
     8881 cgcgcattcc cgtgccgcga ttcatgaaga tcagcgccac cgcgaacagc acgaccgctg
     8941 agagcagcat gatgccgaag gccacgaaca cgttgacgtc gctggtaccg aggaacccgt
     9001 atcggaacgc gttcaccatg tgcaggatgg ggttcgcgta cgacagatgc cgggcccact
     9061 cgggcagcag cgaaatggag tagaacacgc cgccgaaata cgtgagcggc gtgagcacga
     9121 atgtcggaat ccagttcacc tggtcgaagt tcttggcgaa ttgcgcattg atgaagccgc
     9181 cgagcgaaaa caccaccgac gtcaggacca ccgcggcgag aatgacgaac gcgtggtgca
     9241 cggtgagctg cgtgaagatc agcgagacgg ccgtgaccgc gcccccgacc aggaagccgc
     9301 gcaccagccc gccaccgacg taacccagga tgatgatcca gttgggcagc ggtgcgacca
     9361 gcatctcttc gacgtgcttg ccgaatttcg cgccgaagaa tgacgacacc acgttgccgt
     9421 aggagttggt gatgacggcc atcatgatga gcccgggcgc gatgtactgc atgtagggca
     9481 cgccgtccat gagccccaca cgctggccaa tcaaactacc gaagatgacg aagtagagcg
     9541 ccgaggtgac cgcggacggc acgatggtct gcgcccagat gcggatgatg cggccgtatt
     9601 cgcggatgat gatggtcttg aggcctgtcc agcgcgcctg ccggagggac cgcacggaat
     9661 ccgcggcggg ctcttgcgca gcaacgtcgt tcatgacgaa ctcgccgcga cgcccggctt
     9721 gcgctccacc agtttcatga atagctcttc gagccggttg accttgttgc gcatggagag
     9781 cacgccgatt ccctgctcgg tcagcctgga gaacacttca ttgaggctct gctccttgga
     9841 gacctcgacc tctagcgtgt gctcgtcgat gagctcgaaa tcataaccgg gcaggatcgg
     9901 aacggtgcgc acggcttcgc gaagattcag cacgaaggtt tccgcctgca gcttgcgcag
     9961 cagcgtcgac atgcgatcac gctcggcgat cagcccgcca ttgatgatgg cgatgttgcg
    10021 gcacagggtt tccgcttctt cgagatagtg cgtggtcagg atgatggtgg tgccacgcgc
    10081 attgatctcg cgcagaaaat cccacatcga gcggcggatc tcgatgtcga cgcccgccgt
    10141 cggctcgtcg agtatcagca ggcgcggttc gtgcaccagc gcgcgcgcga tcatcaggcg
    10201 gcgcttcatg ccgccggaga ggccgcgcgc gatcttgtta cgcttgtcgg aaagctgcag
    10261 caacgacaga tatttctcgg tgcgttcgcg ggccagcggc cgctcgatgc catagaagcc
    10321 ggcctggttg accatgatgg tctgcggcgt ctcgaactga ttgaaattga tttcctgcgg
    10381 cacgatgccg atgcacgcct tcgccgcttc gagttcgcga tcgagatcgt gaccgaacac
    10441 gctgaccgta cccgcggatt tgttcacgag cgaagtcagg atgccgatga gcgtcgtctt
    10501 gccggcgccg ttaggcccta acaaggcaaa gaagtcgccg gtctccacgt ccaggtcgac
    10561 gcccttgagc gcctgtaccc cgttcttgta ggtcttggtg aggccgcgaa tcgagagtg
//
LOCUS       DASQJS010000186         3841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4500028, whole genome shotgun sequence.
ACCESSION   DASQJS010000186 DASQJS010000000
VERSION     DASQJS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4500028"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1926
                     /gene="glgB"
                     /locus_tag="VEW08_07745"
     CDS             <1..1926
                     /gene="glgB"
                     /locus_tag="VEW08_07745"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HYJ40666.1"
                     /translation="HVVVKHDTYFFSHELSEFDLYLFGEGRHYGLYHKFGAHLRVRDG
                     VAGTHFAVWAPNARRVSVVGSFNQWDGRKHAMQARGGSGVWELFIPGVGEGAEYKYEI
                     RTQRDSLLLKSDPFGFFMQKRPETASVVASLDGYDWHDADWLRDRAHRDWRRSPINVY
                     EVHLSSWQHSWDRQPPFFTWADAAERLIPYVVDMGYTHIELMGVAEHPFDGSWGYQVV
                     GYYAPTARYGTPKEFMAFVDKCHQAGIGVILDWVPAHFPKDAHGLAEFDGSALYEHAD
                     PRLGEHSDWGTKIFNYGRHEVKNFLVANALFWMERYHIDGLRVDAVASMLYLDYSRKA
                     GEWLPNKYGGRENLEALEFLREFNTVAHREHPGVLTIAEESTSFPGVSRPVHLGGVGF
                     SFKWNMGWMNDTLHYFEKDPVYRRHEHNKITFSFMYAWSENFVLPISHDEVVHGKKSL
                     LDKMPGDEWQKRANYRLFLSYMTAHPGKKLMFMGSEFGQWHEWREHEQLDWPLLQNPV
                     HQGLQNLNRELAKFYKAHPQLHASDCDAGGFRWSDLHNADESVFAFLRQEHGFPPIAC
                     VFNATPVPREDYWLGVPDAGRYEVIFDSDHPDFGGAGFAGVAGYEAIAHVVHGFPHAL
                     RMRLPPLGAVFLRRAGG"
     gene            1923..>3841
                     /gene="glgX"
                     /locus_tag="VEW08_07750"
     CDS             1923..>3841
                     /gene="glgX"
                     /locus_tag="VEW08_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HYJ40667.1"
                     /translation="MKEGSPDTLGATFTGEGVNFAVRSRAATRVDVCLYDSTGTRETA
                     RLTLPGRTGPVHHGFVEQELARVGTLYGIRVHGVYDPREGYRFNANKLLIDPWARDIV
                     GDVKWHPSILGYDPTDAEGTRPSLVDSAPAMPRCRVIDGEFDWEEDRAPAVPWRDSLI
                     YELHVKGFTQLHPDVPPEWRGKYLALTVPAVLDHLKSLGVTAVELMPVHAFTSEGFLH
                     ERGLTNYWGYNTVSWFAPTSRYAVRDPVVEFKQAVKALHKAGIEVILDVVYNHTADGN
                     QLGPTLNFRGIDNRAYYFHRANDRRFYDDVTGVGNTVACDHPIVQADILSSLRYFAED
                     FRVDGFRFDLATVLGRDRSGFNSNSPFFAMLAADPVLAYVKLIAEPWDVGLGGYQLGN
                     FPAGWSEWNDRYRDTMRSFWHGGRRMLGGYAERFAGSSDLFRRNGRKPTASINFIAAH
                     DGFTLRDTVSYNDKHNEANLENNRDGHSNNHSWNCGVEGRTDDVHVIALRRRQVRNML
                     TTLFFSQGVPMLLAGDELYRTQNGNNNAYCQDNETSWVDWGGLKADDSLLQFVRALSA
                     LRRARPELRRDTFLKGALQASHSRDICWWHPAGHEMADQEWNNPDLRCLGVGLGGTAS
                     SPDLMLLLNPTESEC"
BASE COUNT          692 a         1238 c         1214 g          697 t
ORIGIN      
        1 cacgtggtcg tcaaacacga cacctatttc ttctcgcacg agctctcgga attcgatctc
       61 tatctattcg gtgaaggccg gcactatggc ctgtaccaca agttcggtgc gcatcttcgc
      121 gtgcgcgacg gcgtcgccgg cactcacttc gccgtctggg cgccgaatgc caggcgagtg
      181 agcgtggtcg gcagcttcaa ccagtgggat ggccgcaagc acgccatgca ggcgcgcggt
      241 ggctcgggtg tctgggagct gttcatcccg ggcgttggcg agggcgcgga atacaagtac
      301 gagatccgca cccagcgcga ctctctgctg ctcaagtccg atcctttcgg tttcttcatg
      361 cagaagcggc ccgagacggc gtccgtcgtc gcctcactcg atggctacga ttggcacgac
      421 gccgactggc tgcgagatcg cgcgcaccgg gactggcggc gttcgccaat caacgtctac
      481 gaggtgcacc tgtccagctg gcagcactcc tgggatcgcc agccgccgtt cttcacgtgg
      541 gcggacgcgg ccgagcggct cattccctac gtcgtcgaca tggggtacac gcacatcgaa
      601 ctcatggggg tggccgaaca tccgttcgac ggctcgtggg gctaccaggt ggtgggctac
      661 tacgcgccga ccgcgcgcta cggcacgccg aaggaattca tggccttcgt cgacaaatgc
      721 caccaggctg gcatcggcgt gatcctcgac tgggttccgg cgcactttcc gaaggacgcg
      781 cacggtctcg cggaattcga cggatccgct ctgtacgaac acgccgaccc gcggctcggc
      841 gaacacagcg actggggcac gaagatcttc aactacggcc gccacgaggt gaagaacttc
      901 ctggtggcga atgcgttgtt ctggatggag cgttaccaca tcgacgggct gcgcgtggac
      961 gccgtggcgt ccatgttgta cctggactac agccgcaagg cgggcgagtg gctgcccaac
     1021 aagtatggcg gccgcgagaa tctcgaggcg ctcgagttcc tgcgcgagtt caacaccgtg
     1081 gcgcatcgcg aacatccggg tgtgctgacg atcgccgagg aatccacttc ctttccgggc
     1141 gtgtcgcggc cggtgcacct cggcggcgtc ggattctcgt tcaagtggaa catggggtgg
     1201 atgaacgaca ccctgcatta cttcgagaag gaccccgtct accgccgtca cgagcacaac
     1261 aagatcacgt tctcgttcat gtacgcgtgg agcgagaact tcgtgctgcc gatctcgcac
     1321 gacgaagtgg tgcacggcaa gaaatcactg ctcgacaaga tgcccggcga cgagtggcag
     1381 aagcgcgcca actaccgcct gttcctgtcg tacatgacgg cgcacccggg taaaaaactc
     1441 atgttcatgg gctcggagtt cggccagtgg cacgagtggc gcgaacacga gcagctcgac
     1501 tggccgctgc tgcagaaccc cgtgcaccag ggcctgcaga acctcaatcg cgagctcgcg
     1561 aagttctaca aggctcaccc gcagctgcat gccagcgact gcgacgccgg cggtttccgc
     1621 tggagcgacc tgcacaacgc cgacgaaagc gtgttcgcgt tcctgcgcca ggaacacggg
     1681 tttccgccca tcgcctgcgt gttcaatgcc actcccgtgc cgcgcgagga ctactggtta
     1741 ggcgtgccgg atgcggggcg ctacgaagtg atattcgact cggatcatcc tgattttggc
     1801 ggcgccggct tcgcaggtgt ggcgggatac gaggccatcg cacacgtcgt tcacggtttc
     1861 ccccacgcgt tgcggatgcg cctgccaccg ctcggggcgg tattcctgag gcgcgccggc
     1921 ggatgaaaga gggttcgccc gataccctgg gggccacgtt taccggcgag ggcgtgaatt
     1981 tcgccgtgcg ctcccgtgcc gccacgcgcg tggatgtgtg cctgtacgat tccaccggaa
     2041 cgcgcgagac cgcgcggctc acgttgccgg gccgtaccgg ccccgtgcat cacggcttcg
     2101 tcgaacagga gctggcgcga gtcggcacgc tgtacggcat ccgcgtgcat ggggtgtacg
     2161 acccgcgcga aggctaccgc ttcaatgcca acaagctgct catcgacccg tgggcgcgag
     2221 acatcgtcgg cgacgtgaag tggcatccgt ccatactcgg ttacgacccg actgacgccg
     2281 agggcacgcg accgagcctc gtcgacagcg cacctgccat gccgcgatgc cgcgtcatcg
     2341 atggcgagtt cgactgggaa gaagaccggg cacccgcggt gccctggcgc gacagcctga
     2401 tctacgagct gcacgtcaag ggcttcacgc agctgcatcc ggacgtgccg cccgagtggc
     2461 gcggaaaata tctggccctc acggtgccgg cggtactcga ccacctgaaa tcactgggcg
     2521 tgaccgcggt cgagctcatg ccggtacacg ccttcaccag cgagggcttc ctgcacgaac
     2581 gcggcctcac taactactgg ggttacaaca cggtgtcatg gttcgcgccc accagccgtt
     2641 acgcggtcag ggaccccgtg gtcgagttca agcaggcggt caaggcactg cacaaggcgg
     2701 gcatcgaggt gatcctcgac gtggtgtaca accacaccgc cgatggtaat cagctgggcc
     2761 ccacgctcaa cttccgcggc atagacaacc gcgcgtatta ctttcaccgt gccaacgacc
     2821 ggcggttcta cgacgacgtc accggcgtcg gcaataccgt cgcgtgcgat cacccgatcg
     2881 tgcaggccga tatcctgtct tcgttgcgct acttcgcgga agatttccgc gtcgacgggt
     2941 ttcgcttcga ccttgcaacc gtgctcggac gcgaccgttc aggtttcaat tccaactcgc
     3001 cgttcttcgc gatgctggcc gccgacccgg tgctcgccta cgtgaagctc attgccgaac
     3061 cctgggatgt gggcctcggc ggctaccagc tcggaaattt cccggcgggg tggagcgaat
     3121 ggaacgatcg ctatcgcgac accatgcgtt ccttctggca cggcggccga cgcatgctgg
     3181 gcgggtacgc ggaacgcttc gcgggttctt cagacttgtt ccggcgcaac ggccgcaagc
     3241 ccaccgccag catcaacttc atcgccgcgc atgacggttt cacgctgcgc gatacggtgt
     3301 cgtacaacga caagcacaac gaagcgaatc tcgagaacaa ccgcgacggt cactccaaca
     3361 atcacagctg gaactgcggt gtcgagggtc gcaccgacga cgtacatgtc atcgcgctgc
     3421 gtcgccgcca ggtgcgcaac atgctgacca cgctgttctt ctcgcagggc gttccgatgt
     3481 tgttggccgg cgacgagctg tatcgcacgc agaacggcaa caacaatgcg tattgccagg
     3541 acaacgagac cagctgggtg gattggggcg ggctcaaggc cgacgattcg ctgctgcagt
     3601 tcgtgcgcgc cctgtctgca ctgcgccgcg cgcgcccgga gctgcgtcgc gacacctttc
     3661 tcaagggtgc gttgcaggcc agtcactcgc gcgacatctg ctggtggcat cctgccggcc
     3721 atgagatggc cgaccaggag tggaacaacc cggacctgcg ttgtctgggt gtcggtctcg
     3781 ggggcacggc atcgtcgccg gacctcatgc tgttgctcaa tcccacggag tcagagtgcg
     3841 a
//
LOCUS       DASQJS010000187         9634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2303719, whole genome shotgun sequence.
ACCESSION   DASQJS010000187 DASQJS010000000
VERSION     DASQJS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9634
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303719"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VEW08_07755"
     CDS             <1..391
                     /locus_tag="VEW08_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HYJ40668.1"
                     /translation="HLESNESPPAEPGKGSTRMLIEIRVVENSWYLDAFMIGAGYRVT
                     MMAPDRLFPVGRWYHAAATFDGQFFRSFVNGELQMEMATPFTPQGPGRASVGARMNQV
                     TYFKGAIHQARFTHEALPAKAFSQRLP"
     gene            complement(404..1600)
                     /locus_tag="VEW08_07760"
     CDS             complement(404..1600)
                     /locus_tag="VEW08_07760"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ40669.1"
                     /translation="MAESPYRWVIVALGGLMGCVSIGAMFSLAVFLAPMSAETGWSRA
                     GISFAMTINFLVMAFGSFGWGAASDRWGARICTLLGAGLLGLALVLASHAKTLIGFQL
                     TYGVLVGLSASAFFAPMIATVTGWFETRRNLAVSLVSAGMGMAPLTISPLASWLITHY
                     GWRAAMFDIGLLAWLVVIPAALFVRRPPMVIQASAQATAIDDRGITVSQALTSPQFLI
                     LGFTFFLCCAAHSGPIFHVVSYAMYCGLGSMAAVSVYSVEGLAGLGGRLLLGVLADRY
                     GAKRILITALAVQAMAIAAYLAVNELEEFYALAIIFGVAYGGGMPLYASLARDYFGQR
                     IMGTVFGAATMLSSLGMSFGPVAGGWVFDHFGTYAWLYIGSAAVAVGAVMLALLFPKP
                     MRIAAA"
     gene            complement(1593..2003)
                     /locus_tag="VEW08_07765"
     CDS             complement(1593..2003)
                     /locus_tag="VEW08_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016144070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ40670.1"
                     /translation="MDIGEVARRAGIPASTLRFYEEKGLVASVGRRGLRRQYDRDVLE
                     RLALIALGRSAGFSLSEIARMFAPNGRLQVDRPMLVKKADELDLTIRKLGAMRDGLRH
                     AAACRAPSHMECPTFRRLLKISAETTARKKKLRG"
     gene            2054..2533
                     /locus_tag="VEW08_07770"
     CDS             2054..2533
                     /locus_tag="VEW08_07770"
                     /inference="COORDINATES: protein motif:HMM:NF022606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 domain-containing protein"
                     /protein_id="HYJ40671.1"
                     /translation="MNENDIVLMLLTGAGATALTDLWAILRRRIFGTPLPNFGFVGRW
                     IAYMAQGRFRHDSIAAAPPVRAERAIGWITHYATGMAFALLLPAFRGPEWIQHPTLLP
                     ALIVGVGTVAAPYFVMQPAMGLRPSATARLQSLITHAVFGLGLYLAGKIVSSLFTGG"
     gene            2535..2756
                     /locus_tag="VEW08_07775"
     CDS             2535..2756
                     /locus_tag="VEW08_07775"
                     /inference="COORDINATES: protein motif:HMM:NF022833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HYJ40672.1"
                     /translation="MRIPARFAPIVFGALLSAIMVAIVSAFVLASTQGIQSDFALRWL
                     RSCALTWPVAFPTVTLLAPWVRRAVSAIT"
     gene            complement(2762..3646)
                     /locus_tag="VEW08_07780"
     CDS             complement(2762..3646)
                     /locus_tag="VEW08_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ40673.1"
                     /translation="MKRVLEGRVAIVTGAGGGLGREHALFLARSGARIVVNDRLREAA
                     EAVANEIMAAGGTAICQAASVTDEAAIGSMVGRTMAEWGRVDILINNAGILRDKSFAK
                     MSLDDFRAVVDVHLMGAAICTKAVWDHMREQKYGRIVMTTSSSGLYGNFGQANYGAAK
                     MALVGLMHTLAIEGEKYGIRVNCLAPTASTQMTHGVLPEASLRLLDPALVSPALLALV
                     GEGAPTRAIVCAGAGHFARANVTLTHGCHVGGGDDAAARVIAQWDEISDREGEIVPEY
                     GFKQAEREVASANRLNEP"
     gene            complement(3643..4323)
                     /locus_tag="VEW08_07785"
     CDS             complement(3643..4323)
                     /locus_tag="VEW08_07785"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYJ40674.1"
                     /translation="MAKKQKTRRRPTKVQQRAEKMEQILDAAEYLFSKHGLYGVTLKD
                     VAKRVGAHHTLLNYYFVDKKKLFDAVFARRAVVTSTKRMEALERYENEAQGRPTVEGA
                     LRAFLDTDLDLYIEGGESWKNYGALGAQVANTPEWGAELMDAQFDPVVLRLIKLLKKA
                     LPDSDEQDIFWGYHFVTGALMLTLARTGRIDKLSAGKCRSEDFAAVKERMASFMAAGF
                     LEICKKQN"
     gene            complement(4316..5911)
                     /locus_tag="VEW08_07790"
     CDS             complement(4316..5911)
                     /locus_tag="VEW08_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYJ40675.1"
                     /translation="MQMIDGEMQPFALTLDKFLEHAAKWYPQAEVVTARAGGHVDRIG
                     YGQLDRRSRKVSAVLAGFDVQMGDRVATLAWNTQAHVEVWYAIMGMGAVCHTLNPRLT
                     CAQLASMVAQSGARVLVTSADLEPLARKIAAAAPSIERLLLIDVAQEVAATSNAELIA
                     WALEPLIERVRDEVLWAGFDETAPCGLCFTSGTTGAPKGVTYTHRSSFLHTLRLLQVD
                     GMAIAARDSVLVAVPMFHANAWGLPFATPAVGAKLVLPGRQTDGASLAELINREAVTV
                     AVGVATVWLDLVEHVESTGIDLPTLERIIVGGAPLPPALMKRIEHRLGVTIQTSWGMT
                     ELSPSGTVAPRDALLRSAHLSGRPAVGVDLLLTDAGGQALPEQRGVEGHLRVRGASVI
                     ERYFGEEQKATDADGWFPTGDLARIDAAGNLIITGRAKDLIKSGGEWINPAEIEAVVS
                     ALPEVSLAAVIGRTHLKWGERPILVVETHGPINDEALLAPLRGKVAPWWIPDEVIRVP
                     RMHLSPTGKIDKVRLRMEYGGSG"
     gene            complement(5902..7530)
                     /locus_tag="VEW08_07795"
     CDS             complement(5902..7530)
                     /locus_tag="VEW08_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HYJ40676.1"
                     /translation="MTRIAFLFVALCLFSSAGADAPPVVAAPAGKMQGETAGGLHIFR
                     GIPYALAPTGAMRWKPPLPAAKWKGTRDATKFGPVCVQPKPRPGSIYTWELPAMSEDC
                     LSLNIWAPVNAQKAPVFVWIHGGALTSGAGSDKLYDGARLASQGIVVVTINYRMGVLG
                     FLAHPALSAESRRNVSGNYGLLDQIAALRWVSRNIAAFGGDASNVTIAGESAGGLSVM
                     YLMATPEARGLFAKAVAQSAYMISAPELRTANFGGVAAEPVGPWLGEKVGAGDLPGLR
                     SMDAVAITDAAASAGFFPFFVVDGRVLPRQVVELFDRGEQPKIPILAGFNSGEIRSLR
                     FLMPPAPADAAAYEARIRANYADLAEPFLKQYPSGDFAESMLATTRDALYGWTAERLV
                     AMQTAAGVPSFLYYFDHGYPAADSQNLHAFHAAEIPYVFGTADKTPANWPKVPATPVE
                     TQLTDAMLSYWATFARSGVPTAKGQPAWPAYGTERAYMAFEESPRPKVRLLPGMYELN
                     EQVVCRRRAKGGIPWNWNVGLASPPLPAAEASCK"
     gene            complement(7527..8855)
                     /locus_tag="VEW08_07800"
     CDS             complement(7527..8855)
                     /locus_tag="VEW08_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ40677.1"
                     /translation="MDAEAKSPTPLHVAALRARPDARAWTALGILCFVYVLNFLDRQL
                     LSILAKPIQDDLGVTDGQLGRISGLYFALFYCVLAIPVGWFADRTNRVRVLSFACALW
                     SAATVACGMTTNYPQLVAARMSVGVGEAGGVPPSYAIISDYFPPGTRGTALSLFNLGP
                     PIGLALGVAFGASIAATYSWRSAFICLGTVGIVAALVVLLVVREPVRGGLDVPRAGGV
                     SGVSAKAAFWPTCRMFFSNPVLLRVSLATGATQFVTYALLNFTVLFLMREKGMTLQDV
                     AVYYALVVGIAIGAGMYTSGRLIDRFAPRSRTAYAYLPAIALALAIPAFIGFVRAPGW
                     PLALAFLAVPTFLNYFYLSPAVALVQEQVQPHERVLAGALLLLVMNLIGLGLGPTFLG
                     AVSDYFRPTHPDHSLQMAFYTLVPFYIIAVLSFLWLARALKREAPGGDIP"
     gene            complement(8858..>9634)
                     /locus_tag="VEW08_07805"
     CDS             complement(8858..>9634)
                     /locus_tag="VEW08_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ40678.1"
                     /translation="NPHHFVYAFVATGYRPGGLNVPVGLGPPPPFDEEEVTNYEIGWK
                     SGWMDGRLRLQLDAFYNDYDNFQVTVGYPDIPVFGFELNNPSTTEIYGAEAQLEAVFG
                     AFTLDAGVGWLQSSLGEFYAVDPRVPSFGACDPETGPASAACINLAGHDQTYAPDFTF
                     NLGMQYAFSLGNGDQITPRFNYGHVSEQWATLFQNEARGDLVEARNIVNAQITWKHGD
                     FFTTLFGSNLTDQHYVGAVNSGLRFAGAPMFYGLRVTKAF"
BASE COUNT         1794 a         3190 c         3027 g         1623 t
ORIGIN      
        1 gcacctggaa tccaatgagt ccccgccggc cgagccgggc aaaggcagca cccggatgct
       61 gatcgagatc cgggtcgtcg aaaacagctg gtacctggac gccttcatga tcggcgccgg
      121 ctaccgcgta accatgatgg ccccggacag actgtttccc gtcggacgct ggtatcacgc
      181 ggccgcgacc tttgacggcc agtttttccg ctcattcgtg aacggagagc tgcagatgga
      241 gatggcgaca cctttcacgc cgcaagggcc cggacgcgcc tcagtgggcg cgcgaatgaa
      301 tcaggtcacc tatttcaaag gcgccattca ccaggcgaga ttcacgcacg aggcgctgcc
      361 ggcaaaggca ttcagtcagc ggcttccata ggaacgggcc ggatcaagcc gccgcgattc
      421 gcattggttt tggaaacagc agagccagca ttaccgctcc cacggcaact gccgccgaac
      481 ctatgtaaag ccatgcgtag gtaccgaagt ggtcgaacac ccatccaccc gccaccggcc
      541 cgaatgacat gccgaggctc gagagcatgg tggccgcgcc gaacaccgta cccatgatgc
      601 gctggccgaa atagtcgcgt gcgagtgacg cgtacagcgg catgccgcct ccataggcga
      661 cgccgaagat gatcgcgagc gcgtagaact cttcgagttc atttaccgcg aggtaggctg
      721 cgatcgccat cgcctgcaca gcgagagcag tgatcagaat gcgcttcgca ccgtagcgat
      781 ccgcgagcac gccgagcagc agtcgcccgc cgagccctgc gaggccttcg acgctgtaga
      841 cgctcaccgc cgccatcgaa cccagcccgc aatacatggc gtagctgacg acgtggaaga
      901 taggccccga gtgcgccgcg cagcacagga agaacgtgaa tccgaggatg aggaattgcg
      961 gagaagtcag tgcctggctg actgtgatcc cgcgatcatc gatggcggtc gcctgtgctg
     1021 acgcctggat gaccatcggc ggccgccgca cgaacagcgc cgccgggatc acgacgagcc
     1081 acgcgagcaa gccgatatcg aacatcgccg cgcgccagcc gtagtgagtg atcaaccagc
     1141 tggccagggg cgagatggtg agcggcgcca tgcccatgcc ggccgacacc agcgacaccg
     1201 ccaggttgcg ccgggtttcg aaccagccag tgaccgtggc gatcatcggt gcgaagaacg
     1261 cgcttgccga caggccgacc agcaccccat aggtgagctg gaagccgatg agtgtcttcg
     1321 cgtgactcgc gagcaccagc gccagcccga gcagcccggc acccagcagc gtgcagatgc
     1381 gcgcacccca tcgatcgctc gccgcacccc agccgaaaga gccgaaagcc atgacgagga
     1441 agttgatcgt catcgcgaaa gaaattccgg cgcgcgacca gccggtttcg gcggacatcg
     1501 gcgccaggaa caccgcgagc gagaacatgg cgccgatcga gacgcagccc atgaggccgc
     1561 cgagcgccac gatgacccag cgatatggag attcagccac ggagtttttt ctttcgcgcg
     1621 gtggtctcgg ccgagatctt cagcaaccgc cggaacgtcg ggcattccat gtggctcggc
     1681 gcgcggcagg cagcggcgtg tcgcaagcca tcgcgcatgg caccgagctt gcgaatggtg
     1741 agatcgagct catccgcctt tttcacgagc atggggcggt cgacctgaag gcggccgttg
     1801 ggcgcgaaca tgcgcgcgat ctcgctgagc gaaaaacccg ccgagcgccc aagcgcaatg
     1861 agcgcgaggc gttccaaaac gtcgcggtcg tattggcgtc gcaggccgcg ccggcccacc
     1921 gatgcgacca ggcccttctc ttcgtaaaaa cgcagcgtcg aggcgggaat accggctcgc
     1981 cgcgcgactt cgccaatgtc cagatcacgc atcgcttgac ctcaagtgga cttcaagtgc
     2041 gactctacca gtcatgaacg aaaacgacat tgtattgatg cttctcacgg gcgccggcgc
     2101 cacggccctg accgatctct gggcgatatt acgcaggcgc attttcggga cgccgctgcc
     2161 caacttcggg ttcgtgggcc ggtggatcgc atacatggcg caaggccgat ttcgccacga
     2221 ttccattgcg gcggcgccac cggttcgcgc ggagcgcgcc atcggctgga tcacccacta
     2281 cgcgacaggc atggcatttg cgctactgct ccccgcattc cgcggcccgg agtggattca
     2341 gcatcccacg ttgctgcctg cgctgattgt cggcgttggc accgtcgccg cgccctactt
     2401 cgtcatgcag ccggccatgg gtctacggcc ctcagccacc gcgcgcctgc aaagtctcat
     2461 cacgcacgcg gtgttcggcc tcggcctcta cctcgccggc aagatagtta gttcgttatt
     2521 cactggagga tgaaatgcgt atacccgccc gattcgcgcc catcgtgttc ggcgccctgc
     2581 tctcggccat catggtggcc atcgtgtccg cgttcgtgct ggcgagcacg cagggcattc
     2641 aaagcgactt tgcgctgcgc tggctgcgaa gctgcgcgct gacctggccc gtggcctttc
     2701 cgacggtgac gctgctcgcg ccgtgggtgc gccgggcggt cagcgcgatt acgtgaccga
     2761 atcacggctc atttagacga ttcgcgctcg ctacctcgcg ctcggcctgt ttgaagccgt
     2821 actcgggcac gatctcgccc tcgcggtcgg agatttcgtc ccattgcgcg attacgcgcg
     2881 cggccgcgtc gtcgccacca ccgacatggc agccatgcgt cagcgtgaca ttggcgcgcg
     2941 cgaagtgtcc cgcgcccgcg cagacgatgg cgcgggtggg cgcaccttcg ccgaccagtg
     3001 ccagcagtgc gggactcacg agcgccgggt cgagcaacct gaggctcgcc tcgggcaata
     3061 ccccgtgcgt catctgcgtc gacgcggtgg gcgcgaggca attgacacga atgccgtact
     3121 tctcaccctc gatggccagt gtgtgcatca ggccgaccag cgccatcttc gcggcgccgt
     3181 agttagcctg tccgaagttt ccgtacaagc ctgacgacga ggtagtcatg acgatgcgac
     3241 cgtatttctg ttcacgcatg tggtcccaca ccgccttggt gcagatggcc gcacccatca
     3301 ggtggacgtc gaccacggcg cgaaaatcgt ccaggctcat cttggcgaag ctcttgtcgc
     3361 gcaggatgcc ggcgttgttg atcaggatat ccacgcggcc ccactcggcc atcgtgcgcc
     3421 caaccatcga gccgatcgca gcctcatcgg tgacggaggc tgcctggcaa atcgccgtgc
     3481 cgccagccgc catgatttcg ttggccacgg cctctgccgc ctcgcgaagg cgatcattga
     3541 cgacgatgcg agcgccgctg cgcgccagga acaatgcgtg ctcgcggccg agcccgccac
     3601 ccgcaccggt gacgatggcc acgcgcccct cgagtacgcg tttcaattct gcttcttgca
     3661 tatttcgaga aagcccgcgg ccatgaacga ggccatgcgt tctttcaccg cggcgaaatc
     3721 ctccgaccgg catttgccgg ccgagagctt gtcgatgcgt cccgtgcgcg cgagcgtgag
     3781 catcagcgca ccggtgacga agtgatagcc ccagaagatg tcctgctcgt cgctgtcggg
     3841 cagcgctttc ttcagcaatt tgatgaggcg caacaccacc ggatcgaatt gcgcatccat
     3901 gagctcggcg ccccattccg gcgtgttggc cacctgcgcg ccgagcgcgc cataattttt
     3961 ccagctctca cctccctcga tatacaggtc gaggtcggtg tcgagaaacg cgcgtaatgc
     4021 cccttccacc gtcggccggc cctgggcctc attctcatat cgctcgagcg cttccatccg
     4081 cttcgtgctg gtgaccacgg cacgccgcgc gaacacggcg tcaaacagct tcttcttgtc
     4141 gacgaagtag taattgagca gcgtatggtg tgcaccgaca cgcttcgcga cgtccttgag
     4201 cgtgacgccg tacaggccgt gcttggagaa aaggtattcg gccgcgtcga gaatctgttc
     4261 cattttctcg gcgcgctgct gcaccttcgt gggccgccgc cgcgttttct gtttcttagc
     4321 cactgccgcc atattccatc ctcaggcgca ccttgtcgat cttacccgtt ggcgacagat
     4381 gcatgcgcgg cacgcggatg acctcgtcgg gtatccacca cggcgcaacc ttgccccgca
     4441 gtggcgcgag caaagcctcg tcgtttatgg ggccgtgggt ctcgacgacg agaatcgggc
     4501 gctcacccca cttgaggtgc gttcgcccga tcaccgcagc gagtgaaacc tcgggaagcg
     4561 cactaaccac cgcctcgatt tccgcggggt tgatccactc gccgcccgac ttgatcaggt
     4621 ccttggcgcg cccggtgatg atcaggttgc cggcagcatc gattcgcgcg agatcaccgg
     4681 tcggaaacca gccgtccgca tccgtcgcct tctgctcttc gccgaagtag cgttcgatga
     4741 ccgatgcgcc acgcacacgc agatggcctt cgactccacg ctgctcggga agagcttgcc
     4801 cgccggcgtc ggtgagcaac agatcgacgc ccacggcggg tcggcccgac aagtgagccg
     4861 atcgcaacag cgcatcacgc ggcgctaccg tgcccgaagg tgaaagctcg gtcattcccc
     4921 agctggtctg aatggtgact ccgaggcgat gctcgatgcg cttcatgagt gccggaggca
     4981 gcggtgcgcc gccgacgatg atgcgttcga gtgttggcag gtcgatgccc gtactctcga
     5041 cgtgctcgac gagatccagc cacacagtcg cgacaccgac cgccaccgtc accgcctcgc
     5101 gattgatcag ttctgcaagg ctggctccat cggtctgccg ccccggcagc acgagttttg
     5161 cgccgacggc gggcgtcgca aacggcaggc cccaggcgtt ggcatgaaac atcggcacgg
     5221 caaccagcac gctgtcgcgc gcggcaattg ccattccatc cacctgcagc aggcgaagcg
     5281 tgtgcaggaa gctcgaccgg tgcgtgtacg tgacgccctt gggcgcgccc gtcgtgcccg
     5341 aggtgaaaca cagtccgcaa ggcgcggtct cgtcgaagcc cgcccagagc acttcatccc
     5401 gcacccgttc gatgagcggt tccagcgccc aggcgatgag ttctgcattc gacgtcgcgg
     5461 caacctcctg cgcgacgtcg atgagcagca agcgttcgat ggacggcgca gccgcggcga
     5521 tcttgcgcgc cagcggctcg aggtcggcgc tcgtgaccag cacgcgggcg cccgactgcg
     5581 cgaccatcga cgcgagttgc gcacaggtga ggcgcggatt gagcgtatgg caaacggcgc
     5641 ccatgcccat gatcgcgtac cagacttcga catgcgcctg cgtgttccac gccagtgtgg
     5701 cgacgcgatc gcccatctgg acatcgaagc ctgccaaaac cgccgacacc ttccggctgc
     5761 gacgatcgag ctggccataa ccgatgcgat cgacgtggcc gccggcgcgt gccgtgacca
     5821 cttcagcttg cgggtaccac ttggcggcgt gctccaggaa cttgtcgagt gtcagcgcga
     5881 acggctgcat ttcgccgtcg atcatttgca tgacgcctcg gcagcgggca gcggtggcga
     5941 ggcgagcccg acattccagt tccacggaat gccgcccttc gcgcggcggc ggcaaacaac
     6001 ctgttcgttg agctcgtaca ttccgggtaa cagacgcacc ttcggtcgcg gcgattcttc
     6061 gaaggccatg taggctcgtt cggttccgta cgcgggccac gcgggctggc ccttcgccgt
     6121 cggcacaccg gaacgcgcaa acgtcgccca gtagctcagc atcgcgtccg tcaactgcgt
     6181 ttcgactggc gtcgccggca ccttcggcca gttcgccggc gtcttgtcag ccgtgccgaa
     6241 gacatacggg atttcggcgg catgaaacgc gtgcaggttc tggctgtctg ccgccggata
     6301 gccgtgatcg aagtaataga ggaaagaagg cacgcccgcg gcagtctgca ttgcgaccag
     6361 ccgctccgcg gtccagccat acagcgcgtc gcgcgtcgtc gcgagcatgc tctcggcgaa
     6421 atcgccagac ggatactgtt tgaggaaagg ctctgccagg tccgcgtagt tggcgcggat
     6481 ccgcgcttca taagcagccg catccgccgg cgctggcggc atcaggaatc gcagcgagcg
     6541 gatttccccg ctgttgaaac ccgcgagaat cgggatcttc ggctgttcgc cgcgatcgaa
     6601 cagctcgact acctggcgcg gcagaacgcg gccatcgacg acgaagaagg ggaagaagcc
     6661 cgcgctcgcg gcggcgtcgg tgatcgcgac cgcatccatg gaccgcagtc ccggaaggtc
     6721 gcccgcgccc accttctctc ccaaccaggg acccacgggc tcggcggcga caccgccgaa
     6781 gttggcggtc ctgagttccg gcgcggagat catgtaggca ctttgcgcaa ccgctttggc
     6841 aaacaacccg cgcgcttcgg gcgtggccat gaggtacatc acgctcaagc cgcccgcgga
     6901 ctcgccggca atggtgacgt tcgacgcatc gccaccgaag gccgcgatat tgcggctcac
     6961 ccagcgcagc gccgcgatct ggtcgagcag tccgtagtta cccgacacgt tgcgccgcga
     7021 ttccgcgctc aaggcgggat gcgcgagaaa gccgagtacg cccatgcggt agttgatcgt
     7081 cacgacgacg ataccctgtg aggcgagtcg cgcaccgtcg tacagtttgt cgctgcccgc
     7141 gcccgacgtg agcgcgcctc catgaatcca cacgaacact ggcgccttct gcgcgttcac
     7201 cggcgcccag atgttgagcg acaggcagtc ttcgctcatt gccggcagtt cccaggtgta
     7261 gatgctgccg ggccgcggct tcggctgaac gcataccggg ccgaacttcg tggcatcccg
     7321 cgtgcctttc cactttgcgg ccggcagcgg cggcttccag cgcatcgctc ccgtgggcgc
     7381 gagcgcgtat gggatgcctc tgaagatgtg caggccaccg gccgtttctc cctgcatttt
     7441 gcctgcgggt gctgcgacga cgggtggtgc atcggcgcct gccgatgaga acaggcacaa
     7501 cgcgacgaac aaaaacgcaa ttcgggtcac gggatgtctc cgcctggcgc ttcgcgcttc
     7561 aacgctcgcg ccagccacag gaacgacagc acggcgatga tgtagaaggg aaccagagta
     7621 tagaaagcca tctgcagaga atggtccggg tgagtgggcc ggaagtagtc gctgacggcg
     7681 ccgaggaacg tgggcccgag acccagaccg atcaggttca tcaccagcag cagcagcgcc
     7741 ccggcgagca cgcgctcgtg tggctgcacc tgctcctgca ccagcgcaac ggccggcgaa
     7801 agatagaaat agttgaggaa ggtgggcacg gccaggaacg ccagcgccag cggccagccg
     7861 ggcgcccgga caaagccgat gaaggctgga atggcgagcg ccagcgcgat ggctggtaga
     7921 taggcgtacg cggtcctgga gcgcggcgcg aatcgatcga tcaggcgccc ggaggtgtac
     7981 atgccggcgc cgatcgcaat tccgacgacc agcgcgtagt agacggccac atcctggagc
     8041 gtcatgccct tttcgcgcat caggaacaac acggtgaagt tgagcagcgc gtaggtgacg
     8101 aattgcgtcg caccggtcgc cagcgagacg cgcagcagca ccgggttcga gaagaacata
     8161 cggcaggtcg gccagaacgc cgctttcgcg gacactcccg atacaccgcc ggcgcgcggc
     8221 acgtcgagac cgccccgcac gggttcacgc accaccagca atacgaccag cgcggcgacg
     8281 atgcccaccg tgccgagaca gatgaaagcc gaacgccagc tataggtggc cgcgatcgac
     8341 gcgccgaacg cgactccgag agcgaggccg atcggcgggc ccaggttgaa gaggctgagc
     8401 gccgtgcccc gtgtgcctgg agggaagtag tcggaaatga tggcgtacga cggcggcaca
     8461 cccccggcct cgcccacgcc cacggacatg cgcgcggcca ccagttgtgg atagttagtg
     8521 gtcatgccgc acgcgaccgt cgccgcgctc cacagtgcgc aggcgaagct caacacgcgc
     8581 acccgattgg tccggtccgc gaaccagccc accgggatcg ccagcacgca atagaacagc
     8641 gcgaagtaga gcccgctgat ccggccgagc tgaccatcgg tgacgccgag gtcgtcctgg
     8701 atcggcttcg ccaggatgga caacagctgc cgatccagga agttgaggac atagacgaaa
     8761 cacaagatcc ccaacgccgt ccacgcgcgc gcatccggcc gcgcgcgaag cgctgccaca
     8821 tgaagcggcg ttggggactt cgcttcggcg tccattgtca gaacgctttg gtgacccgca
     8881 gaccataaaa catcggtgcg ccagcgaatc gcagtcccga attgacggcg cctacatagt
     8941 gctggtcggt gaggttcgag ccgaacagcg tcgtgaagaa gtcgccgtgc ttccaggtga
     9001 tctgcgcgtt cacgatgttg cgcgcctcga ccaggtcacc gcgggcttcg ttctggaaca
     9061 gagtcgccca ttgttcggac acatgcccat agttgaagcg cggtgtgatc tggtcgccgt
     9121 tgcccaggct gaatgcgtac tgcatcccga ggttgaacgt gaagtccggc gcatacgtct
     9181 gatcgtgccc ggcgagattg atgcaggcgg cgcttgccgg cccggtctcc ggatcgcacg
     9241 cgccgaagct cggcacccga ggatcgaccg cataaaactc gcccaggctg ctctgcagcc
     9301 agcccacgcc ggcgtcgagc gtgaaggctc cgaacaccgc ttccagctgc gcctcggcgc
     9361 cgtagatttc cgtggtgcta ggattgttga gctcgaaacc gaacaccggg atgtccggat
     9421 atcccaccgt cacctggaaa ttgtcgtagt cgttgtagaa ggcatcgagt tgcagccgca
     9481 acctgccgtc catccagccc gacttccagc cgatctcgta gttagtgacc tcttcctcgt
     9541 cgaagggcgg cggcggcccg agcccgaccg ggacgttgag tccaccaggc cggtaacccg
     9601 tggcgacgaa cgcgtacacg aagtggtgcg gatt
//
LOCUS       DASQJS010000188         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3558362, whole genome shotgun sequence.
ACCESSION   DASQJS010000188 DASQJS010000000
VERSION     DASQJS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3558362"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2028
                     /locus_tag="VEW08_07810"
     CDS             <1..>2028
                     /locus_tag="VEW08_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HYJ40679.1"
                     /translation="LFNPESIHRLQKSVRTGSYSTYKSYAELIDDRAKNLSTLRGLLD
                     FKQGESISIDEVESVESIMKRFKTGAMSYGSISKEAHETLAIAMNRVGGKSNTGEGGE
                     DPDRYVPMENGDSKNSAIKQVASGRFGVTSHYLVNAKELQIKMAQGAKPGEGGQLPGT
                     KVYPWIAKTRHTTAGVGLISPPPHHDIYSIEDLAELIHDLKNANREARISVKLVAEVG
                     VGTIAAGVAKAHADVVLISGHDGGTGASPLTSLTHAGLPWELGLAETHQTLVINNLRS
                     RIVVETDGQLKTGRDVAIAALLGAEEFGFATAPLVAVGCIMMRVCHLNTCPAGVATQD
                     PRLRARFEGKPEHAVNFMKFIAQHLREIMAQLGFRKLEDMVGRVDMLEPRAAIDHWKA
                     KGFDFSNILYAPDAGPEIGRFRQIGQDHGIDKSLDVTTLLGLCEPAIARGEKVSAELP
                     VRNVNRVVGTITGSAVTKKWGAEGLPEDTISIKFKGSAGQSFGAFMPNGMSFALEGDA
                     NDYFGKGLSGGKLAVFPSPLATFPSEQNMIVGNVALYGATKGEVFVRGMAGERFAVRN
                     SGVDTVVEAVGDHGCEYMTGGRVVVLGPTGRNFAAGMSGGIAYLLDENNESGARINPQ
                     MVDVERVEDPKEAADLRALIEKHVQYTGSEHGKSILAAWETKLPKFVRVIP"
BASE COUNT          403 a          646 c          665 g          314 t
ORIGIN      
        1 cctgttcaac ccggaatcga tccaccgcct gcagaagtcg gtgcgcacgg ggtcgtattc
       61 cacgtacaag agctacgccg agctcatcga cgatcgcgcg aagaatctat ctaccctgcg
      121 cggcctgctc gatttcaagc agggcgaatc gatatcgatc gacgaagtgg aatccgtcga
      181 aagcatcatg aagcgcttca agaccggcgc catgagttac ggctccattt cgaaggaagc
      241 gcacgagacg ctcgccatcg ccatgaaccg cgtgggcggc aagagcaaca cgggcgaggg
      301 tggcgaagac ccggatcgct acgtgccgat ggaaaacggc gactcgaaga actcggccat
      361 caagcaggtg gcgtcgggcc gcttcggcgt gaccagccac tacctggtca acgcgaaaga
      421 gctgcagatc aagatggcgc agggcgccaa gcccggcgaa ggcgggcagc tgcccggcac
      481 gaaggtatat ccgtggatcg caaagacgcg gcacaccacc gccggcgtcg ggctcatctc
      541 gccgccgcca catcacgaca tctattccat cgaagacctg gccgagctca tccacgatct
      601 caagaacgcg aatcgcgaag ctcgtatcag cgtgaagctg gtcgccgaag tgggcgtcgg
      661 caccatcgcg gccggcgtcg ccaaggcgca tgcggacgtg gtgctcatct caggacatga
      721 cggtggcacc ggcgcatcgc cgctcacctc gttgacgcac gccggcttgc cgtgggagct
      781 gggcctcgcc gagacgcacc agacgctggt gatcaacaat ctgagatccc gcatcgtcgt
      841 cgagaccgac ggacagctga agaccggccg cgacgtggcc atcgctgcct tgttgggtgc
      901 cgaggagttc ggcttcgcca ccgcgccgct ggtggccgtg ggctgcatca tgatgcgcgt
      961 gtgccacctc aacacctgcc ctgcgggtgt ggccacgcag gatccgcgcc tgcgcgcgag
     1021 gttcgagggc aagcccgagc acgccgtgaa tttcatgaag ttcatcgcgc agcatctgcg
     1081 cgaaatcatg gcgcagctcg gcttccgcaa gctcgaagac atggtcggcc gcgtcgacat
     1141 gctcgagccg cgcgccgcca tcgaccactg gaaggccaag ggcttcgact tcagcaacat
     1201 tctctacgcg ccggacgccg gccccgagat cggccgcttc aggcagatcg gccaggatca
     1261 cggcatcgac aagtcgctcg acgtcaccac gttgttaggc ctgtgcgagc ccgcgatcgc
     1321 gcgtggcgag aaggtgtcgg ccgagctgcc ggtgcgcaac gtcaaccgcg tggtgggcac
     1381 catcaccggc agcgccgtca cgaagaagtg gggcgccgag ggtctgcccg aggacaccat
     1441 cagcatcaag ttcaagggct ccgcgggcca gagttttggc gcgttcatgc ccaacggcat
     1501 gagcttcgcg ctcgagggcg acgccaacga ctacttcggc aaggggctgt cgggcggaaa
     1561 actcgcggtg tttccgtcgc cgctggccac gttcccgtcc gagcagaaca tgatcgtcgg
     1621 caacgtcgcg ctgtacggcg ccaccaaggg tgaagtgttc gtgcgcggca tggccggcga
     1681 gcgcttcgcg gtgcgcaatt ccggcgtgga cacggtggtc gaggccgtgg gcgaccatgg
     1741 ctgcgaatac atgacgggcg gacgcgtcgt ggtgctgggt cccacgggcc gcaacttcgc
     1801 ggcgggcatg tcgggcggca tcgcctatct gctcgacgaa aacaacgaat ccggcgcgcg
     1861 catcaacccg cagatggtgg acgtggaacg tgtcgaagat ccgaaggaag ccgccgacct
     1921 gcgtgcgctc atcgagaaac atgtgcagta caccggcagt gaacacggca agagcattct
     1981 cgcggcctgg gaaaccaagc tgccgaagtt cgtgcgcgtg atcccgaa
//
LOCUS       DASQJS010000189         6949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3559733, whole genome shotgun sequence.
ACCESSION   DASQJS010000189 DASQJS010000000
VERSION     DASQJS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3559733"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VEW08_07815"
     CDS             <1..540
                     /locus_tag="VEW08_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40680.1"
                     /translation="SKNPVVKRFFSGQKHVEALIITEVLQAKSIGVVGKRDAGVEVAV
                     DVPSIQQMVGAKVGVTVGTSSQSEVVYEGQKLLTFGFKAFSIGMENGEWNIKGVEPDA
                     EHAFAIGQGLTSVVNKDELVDIDFGTAKPKAKVKAKAKAKSKSKSKESAHGRRNNSRK
                     PGKVRADQLRQKRKRARAK"
     gene            455..1762
                     /locus_tag="VEW08_07820"
     CDS             455..1762
                     /locus_tag="VEW08_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40681.1"
                     /translation="MGVETIPGSQAKYALISYDKSGSEREPNSAQPTSAVVLERAANT
                     PVTDVFFFCHGWKGDVPAAKDQYNRWMGALMASPDLARASQIFPNFSPMLVGLHWPSQ
                     PWGDEDVGESGNSFGPSAAGKSPSDLLAHLTSILGDTEQIVAPLKVIIEGARRNAGAS
                     TLPPEVRKAYKDLNDALGLGSQGVGGPPDADREGFDPDETADEGASSFGDFNLNGLLG
                     PLRQLSYWTMKKRARSIGEGGIHKFLVELQKATATKGTRIHLMGHSFGTIVVSSMIGG
                     PDAQASLPRPIDSVTLVQGAVSLWCYANDIPMKRGTKGYFNRIIKDGKVRGPMSITQS
                     KFDKAVGKLYPFASRINGSAAFDVATFPEYGAIGAFGIQGVPQNALTMLEADKPYSLA
                     KGQIYNVESSKFICKGNGLSGAHSDIAGPQVAHLIWETALASK"
     gene            1773..3230
                     /locus_tag="VEW08_07825"
     CDS             1773..3230
                     /locus_tag="VEW08_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HYJ40682.1"
                     /translation="MSAEDETLSFGIEASTGRPLNTLTDDVIRQMLGGETAGVLDLAL
                     KARNDELSAGGRAYGLAPGEEIVDPNDLSISGWGILFGPNVSQKIKDALAPLIERRKQ
                     ECGALFKVFDADGVRKDETVYDWLKRRKVSLNPVVPDAGVPYYLLIVASPQDVSFEFQ
                     YSLDLYWACGRLWFDTAEEFRRYAESVVKYETDAKPPTTRQLALFAPEHDFDNPTQLF
                     TRQVAEPLCGRQTTKPALGAALKYKLREFLGANATKDNLANILRGKIDGGKPALLFSG
                     GHGMQFDNGHPDQLDTQGALVCQDWAGYGAITDDHWFAAKHVPADANVHGLMHFMFAC
                     HGGGVPRLDNFDRLNNAPREIAPRAFFSKLPQALLAHKNGGALAVLAHMERAWAYGFQ
                     GDGGFPQIQGFRGVLAGLLRGDRIGQATDTFNVGWAAVGTRLTEAQEDLKRDATLPVA
                     PIRKLWIQRDDARNFMVFGDPAVRLRQQEMPPLAQ"
     gene            complement(3166..4584)
                     /locus_tag="VEW08_07830"
     CDS             complement(3166..4584)
                     /locus_tag="VEW08_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40683.1"
                     /translation="MIRPAHVRRLAELRAKLTSLRNELDDWRKLTQDSLPLRRHFKQI
                     RLLDATLTGMLASTSKALAQQAEDESVFVNGIEWENELLAAHSIWEVFRSKYVLRQND
                     TFRDYLAACDDVAWACYEPALRKFAPGVDKEPPLVFLSATWSPFVQSRDTNFQNEIRV
                     TSAGVPALSSDEFQQVLKRLPFPLVSLPWYQAFHLPGANIIAHEVGHVVDNEFDIRAQ
                     ILARLDAANLKFAATWKSWAAEMFADIYGCLAMGPAFAGAMLDLLATNAKEVQNERRL
                     GGAHPTHALRVQIILRALSLSGYGDEALRLRGAWEKSFPPKKIMDDVAAEMDAVVDII
                     LARPYHGANLTEVISLPPSGVVKQMRAYAGQGQALQLRQFDASDPRYLFAAAQLLHEE
                     PIANQKDNAFQLLVAQAMRKGTGQFRSQGKPVATQAELDQQLDAQLTSCSNQGLELRK
                     LLTEPKAASPAASTAPPDPRTP"
     gene            complement(4581..>6949)
                     /locus_tag="VEW08_07835"
     CDS             complement(4581..>6949)
                     /locus_tag="VEW08_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40684.1"
                     /translation="LKVITLAREGLAGVADQTDSEGLRADFHALQGVAHKHLLAYTDA
                     FDCFERAGVVFDEAGSADGAARSTLLAAQLTALDLANIHDALSLVEGAAQLRGARDGE
                     LYVEFQLLRAYAAYRGGNLDTARELMPELRRRCAGQHPRLRARVAMFELCFGLETQER
                     RHLELLAAVQAVEPRSARMKLLDPCGYSEASLNLTPKVRQRLQELFELDAPDSSIHEC
                     LGRAHMFRLMGEPDCAAAVLRGFLVEPAAKDANNFFFCHWLIQQALRQLGESTDYRSL
                     LKPMSLVISSAEGLLNAWWDGLQVESALEAVRAGDFAEAETVAQQGAPFAALAGQMQP
                     LWLSRIEEVQFYSLEHRPPGSHAEQTAERFESMGDSVMARRIIEEWQPFAPQPQMAGA
                     EPPEAPEAPPRMAAPMPSAPLSPPGAPLKMSEPPSFGVPNERPHGSLSTPEPVSESLP
                     SDSRVEAEIQIGTGGLPDLAREALNSNENAIDILLGRWHELADQLSDRLERDVGSVPA
                     TLAFEVAPQAASLPWELAPFIGRCGRYWRKNAHARAPAMPRGSRVDRVLLVRPRQGSG
                     DISLESASGGSLESLYTVRNGAPDFHVLFDPTPEMLIETLQRTRPTIVHINAAMRELQ
                     GGIFLDFESTESRADERGARHGALTKYAQSASVASERRELVWSTTRLGRTLQSAPEPL
                     TLVLDIAASSNLTETIRMLLLRNQFANQVFQFAPMCALVGTGLASEYERFELSRAMID
                     GLLAGGAFGVIRALRDCPTNNVGDLLSALPRLAPALWAHDAHAGVFAS"
BASE COUNT         1358 a         2133 c         2252 g         1206 t
ORIGIN      
        1 tccaagaacc ctgtggtcaa gcgcttcttt tccggccaga agcacgtgga ggcgctgatc
       61 atcaccgagg tgctgcaggc caagtccata ggcgtcgtcg gcaagcgaga cgcgggcgtc
      121 gaggtcgccg tcgacgtgcc cagcatccag cagatggtgg gtgcgaaggt aggcgtcacg
      181 gtgggcacgt catcccagag cgaggtcgtc tacgaaggac agaaactcct caccttcggc
      241 ttcaaggcct tcagcatcgg catggagaac ggcgagtgga acatcaaagg cgtcgagccg
      301 gatgcggaac atgcgttcgc catcggccag gggctcacaa gcgtcgtgaa caaggacgaa
      361 ctcgtggaca ttgatttcgg aactgcaaaa cccaaggcga aggtgaaggc gaaggccaaa
      421 gccaagtcca agtccaagtc caaggagagt gcacatgggc gtagaaacaa ttcccggaag
      481 ccaggcaaag tacgcgctga tcagctacga caaaagcgga agcgagcgcg agccaaatag
      541 cgcacaaccg accagcgccg tagtcctcga gcgtgcggcg aacacgccgg ttaccgatgt
      601 cttcttcttc tgccatgggt ggaagggcga cgtaccggcg gcgaaggatc aatacaaccg
      661 gtggatgggc gcgctcatgg cgtcgcccga tctggcgcgg gcgtcgcaga tattccccaa
      721 cttctcgccg atgctggtgg ggctgcattg gcccagtcag ccgtggggcg atgaagatgt
      781 tggtgagagc ggcaattcgt tcggaccctc ggcggcgggc aaatccccgt cagatctcct
      841 ggctcatctc accagcatcc ttggtgacac ggagcaaatc gttgcgccac tgaaggtcat
      901 catcgaggga gcacgccgca atgcgggcgc ctcaactctg ccgcccgagg tgcgcaaggc
      961 atacaaggat ctcaatgacg cgctcggcct gggatcacag ggagtgggcg gtccgccgga
     1021 tgcggaccgc gaagggtttg atccggacga gacagccgac gaaggcgcga gcagcttcgg
     1081 cgatttcaac ctgaatggat tgttggggcc gttacgccag ctttcctact ggacgatgaa
     1141 aaagcgcgct cgctcgatag gcgaaggcgg cattcacaag ttcctcgtcg agctgcagaa
     1201 ggcgaccgcc accaagggca cgcgcattca cctcatggga cacagtttcg gcaccatcgt
     1261 cgtctcgagc atgataggcg gacccgatgc gcaggcgtcg ctgccacgtc ccatcgactc
     1321 ggtgaccctt gtgcaaggtg cagtgtcgtt gtggtgttat gcgaacgaca ttccgatgaa
     1381 gcgcggcacg aagggatact tcaacaggat catcaaggac ggcaaagtgc gcggcccgat
     1441 gagcatcacg cagtcgaagt tcgacaaggc cgtgggcaag ctctatccat tcgcgagccg
     1501 catcaacggt tccgccgcgt tcgacgttgc gacctttccc gaatacggcg ccattggcgc
     1561 attcggaatc cagggcgtgc cacagaacgc gctcacgatg ctcgaggccg acaagccgta
     1621 ctcgctcgcg aaagggcaga tatacaacgt cgaatcgtcg aagttcatct gcaaaggtaa
     1681 cggcctgagt ggtgcgcaca gcgatatcgc gggtccgcag gtcgcgcacc tgatctggga
     1741 aaccgcgctc gcatcgaagt gagggacgcg cgatgagcgc agaagacgaa acgctgtcat
     1801 tcggcatcga ggcgtccacg ggacggcctc tcaacacgct caccgatgac gtcatcaggc
     1861 agatgctcgg cggcgagact gcgggcgtgc tcgatctcgc gctgaaggcg cgcaacgacg
     1921 agctctcggc aggcggcaga gcttacggat tggctcccgg tgaagagata gtggatccca
     1981 atgacctcag catctcgggc tggggaatcc tcttcggccc gaacgtcagc cagaagatca
     2041 aggacgcgtt ggcgccactc atcgagcggc gcaaacagga atgcggtgcg ctgttcaagg
     2101 ttttcgatgc ggacggcgta cgcaaggacg agacggtgta cgactggctc aagcgtcgca
     2161 aggtgagcct gaaccccgtc gtgcccgacg ccggcgtgcc ttactacctg ctcatcgtcg
     2221 catcaccgca ggacgtgagt ttcgagttcc agtactcgct cgatctctac tgggcctgcg
     2281 gccggttatg gttcgacacc gccgaagaat tccgccgcta cgccgaaagc gtggtgaagt
     2341 acgagaccga tgcaaaaccg cccaccacgc gtcagctcgc gttgttcgcg cccgaacatg
     2401 acttcgacaa tccgacgcag ctgttcacgc gccaggtagc cgaaccgctg tgcggacggc
     2461 agacgacgaa gccggcactg ggggcggcgc tcaagtacaa gctgcgcgaa ttcctgggcg
     2521 ccaacgccac gaaggacaac ctggccaaca tcctgcgcgg caagatcgac ggcgggaagc
     2581 cggcgctgct cttcagtggt gggcacggaa tgcagttcga taacggccat ccggatcaac
     2641 tcgacacgca gggcgcgttg gtgtgccagg actgggctgg ttacggcgcc atcacggacg
     2701 atcactggtt cgcggcgaag cacgtaccgg ccgatgcgaa cgtgcacggc ctcatgcatt
     2761 tcatgttcgc gtgccatggc ggaggtgtgc cgcggctcga caatttcgat cgcctgaaca
     2821 acgcccctcg cgagattgca ccgcgcgcat tcttctcgaa gctgccgcag gcgctcctgg
     2881 cccacaagaa tggcggcgcg ctcgccgtgc tcgcgcacat ggagcgcgcc tgggcctacg
     2941 gcttccaggg tgacggtggc ttcccgcaga tccagggatt tcgcggagtt cttgcgggcc
     3001 tgctgcgcgg cgatcgaatc ggtcaggcga ccgatacgtt caatgtaggc tgggccgcgg
     3061 tgggcacgcg cctgactgaa gcgcaggagg acctcaagcg cgatgcgact ctgcccgtcg
     3121 cgcccatccg caagttgtgg atccagcgcg acgacgcgcg caacttcatg gtgttcgggg
     3181 atccggcggt gcggttgagg cagcaggaga tgccgccttt ggctcagtga ggagtttgcg
     3241 cagttcgaga ccctggttcg aacacgacgt cagttgcgcg tcgagttgct ggtcgagttc
     3301 cgcctgcgtg gcaacgggtt tcccctggct gcgaaattgc ccggtgccct tgcgcatggc
     3361 ctgcgccacc agaagctgga acgcgttgtc cttctggttg gcaatcggct cttcgtgcag
     3421 gagttgggcc gcggcgaaca ggtagcgtgg atcgctcgca tcgaattggc gcaactggag
     3481 tgcctgaccc tgtccggcgt aggcgcgcat ctgcttcacc acgcccgatg gcggcagcga
     3541 gatcacctcg gtgagattcg caccgtggta ggggcgcgcc aggatgatat cgacgaccgc
     3601 gtccatttca gccgccacgt cgtccatgat ctttttcggc ggaaaactct tctcccaggc
     3661 gccgcgcaag cgaagtgctt catctccata gccggagagc gaaagtgcgc gcaggatgat
     3721 ttgcacacgc aacgcgtgtg tcggatgagc gccaccaagc cggcgttcgt tttgaacttc
     3781 ctttgcattg gtagcgagca gatcgagcat ggcgccggcg aatgccggac ccatcgcgag
     3841 gcagccgtag atgtctgcga acatttccgc cgcccagctt ttccaggtcg cggcgaactt
     3901 gagattggcc gcgtcgagtc gtgcgaggat ttgcgcgcgg atgtcgaact cgttgtccac
     3961 gacatggccc acctcatgcg caatgatgtt cgctcccggc agatggaagg cctggtacca
     4021 gggcaggctc accagcggaa agggcaggcg cttcaacacc tgctggaact catcgctgct
     4081 caacgctggg acgccggcgg aagtgacccg aatttcgttc tggaaattcg tgtcacgcga
     4141 ttgcacgaac ggactccagg tcgccgacag gaacacgagc ggaggttctt tatccacgcc
     4201 aggcgcgaac ttgcgcaggg ccggctcgta gcaggcccag gcaacatcgt cgcaggcagc
     4261 gaggtaatcg cgaaacgtat cgttctggcg caacacgtac tttgaacgga aaacctccca
     4321 gatcgaatgc gcggccagca gttcgttttc ccattcgatg ccgttgacga atacggattc
     4381 gtcctcggcc tgttgggcaa gcgccttgct cgtagaagcg agcatgccgg tgagcgtggc
     4441 gtccagcagg cgtatctgct tgaagtggcg tcgcagcggg agagagtcct gggtgagctt
     4501 gcgccagtca tcgagctcgt tgcgcaggct cgtgagcttc gcgcgtagct ccgcgaggcg
     4561 ccgcacatgg gccggacgaa tcacgaagcg aacacgcccg cgtgtgcgtc gtgagcccag
     4621 agggcaggcg cgaggcgggg cagcgcgctc aacaggtcgc caacattgtt cgtcggacag
     4681 tcgcgtaacg cgcgaatgac gccgaacgca ccgccggcca acaggccatc gatcatggct
     4741 cgcgagagtt cgaagcgttc gtattcggat gcgaggccgg taccaaccag cgcgcacatg
     4801 ggcgcgaact ggaacacctg atttgcgaat tgatttcgca gcaacagcat gcggatcgtt
     4861 tcggtgagat tcgaagaggc cgcgatgtcc aggaccagcg tcagcggctc cggcgccgac
     4921 tgcagcgtgc ggccgagacg cgtggtgctc cacacgagct cgcgacgctc tgaggcgacg
     4981 gacgcgctct gggcatactt cgttaaggcg ccatgacgcg cgccgcgctc gtcggcgcgc
     5041 gactcggtgc tttcgaagtc gaggaaaatg ccaccctgca attcgcgcat ggccgcattg
     5101 atatgcacga tggtgggccg tgtgcgctgc agggtttcga tcagcatctc cggggtcgga
     5161 tcgaacagca catggaagtc gggtgcgcca ttgcggacgg tgtacagcga ttcgagcgag
     5221 ccgccggagg cggattcgag actgatgtcg cctgaaccct ggcgcgggcg caccagcaac
     5281 accctatcta cccgactccc acgcggcatc gcgggcgcac gcgcgtgtgc gttcttgcgc
     5341 cagtagcgcc cgcagcggcc gatgaaaggc gcgagttccc atggcaggct tgcggcctgc
     5401 ggcgccacct cgaaggcgag cgtggcgggc accgagccca catcacgctc aaggcgatcc
     5461 gataactggt cggcgagctc gtgccaacgt ccgagcagga tgtcgatagc attctcattc
     5521 gagttcaacg cctccctggc gagatcgggg aggccgccag tccctatctg gatctccgct
     5581 tccaccctgg aatccgaagg cagcgattcg ctcacgggct cgggcgtcga caagctaccg
     5641 tgtgggcgtt cattcggcac accgaagctg ggcggctcgg acatcttgag cggagcgccg
     5701 ggaggtgaga gcggcgctga cggcatcgga gcagccattc gcggcggagc ttcaggggct
     5761 tccggcggtt cggcgcccgc catctgcggc tgcggcgcga acggctgcca ctcctcgatg
     5821 atccgccgcg ccatcaccga gtcgcccatg gactcgaagc gttccgcggt ttgttcggcg
     5881 tgagaccctg gcggccggtg ttcgagggaa tagaactgca cttcctcgat gcgcgacagc
     5941 cacaacggct gcatttgccc ggcgagtgcc gcgaagggcg caccctgttg ggcaacggtc
     6001 tcggcctccg cgaaatcccc ggcgcgcacc gcttcgagcg cactctccac ttgcaagcca
     6061 tcccaccagg cgttcaacag cccttccgca ctggaaatta cgagggacat cggtttgagc
     6121 aggctgcggt agtcggtcga ttctcccaac tggcgcaacg cctgctggat cagccagtga
     6181 cagaagaaaa agttgtttgc gtccttcgcg gcgggctcga cgagaaatcc gcgcagcacc
     6241 gcggccgcgc agtccggctc acccatgagc cggaacatgt gcgcgcgacc caggcactca
     6301 tggatcgaag agtccggcgc gtcgagctcg aacaattcct ggaggcgttg tcgaaccttt
     6361 ggcgtcaggt tcaatgaagc ttcgctgtat ccacacgggt ccagaagttt catgcgcgcc
     6421 gacctgggct cgacggcctg cacagcggcg agcagttcca ggtggcggcg ttcctgggtc
     6481 tcgagaccaa aacagagctc gaacatcgcc acgcgtgcgc gcagtcgcgg atgttgtcct
     6541 gcgcagcggc ggcgcagctc aggcatcagc tcgcgagccg tgtccaggtt cccgccacga
     6601 tatgcggcgt acgcccggag cagctggaat tccacgtaga gttcgccgtc ccgcgcgccg
     6661 cgaagttgcg ccgcgccttc cacaagcgac aacgcatcgt gaatgttcgc caggtccagc
     6721 gccgtcagct gggccgcgag cagtgtgctg cgggccgcgc catcggccga gcccgcttca
     6781 tcaaagacca cgccggcgcg ctcgaaacag tcgaaggcgt cggtataggc aagtagatgt
     6841 ttgtgcgcga ctccctgcag cgcatggaaa tccgctcgca agccctcgga gtcggtctga
     6901 tcggccacgc cggccagacc ttcgcgtgcc aaggtgatca ccttcaaca
//
LOCUS       DASQJS010000190         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1046488, whole genome shotgun sequence.
ACCESSION   DASQJS010000190 DASQJS010000000
VERSION     DASQJS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046488"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VEW08_07840"
     CDS             complement(<1..191)
                     /locus_tag="VEW08_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40685.1"
                     /translation="MNAVSNMSGSRLWAAYLGEMRFEFIKSLRTPAFAVPTLLFPILF
                     YVMFGIFFGSMRGNSGQAA"
     gene            complement(188..1090)
                     /locus_tag="VEW08_07845"
     CDS             complement(188..1090)
                     /locus_tag="VEW08_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ40686.1"
                     /translation="MTQTALASLKGVRKRFGKIQALDGFDLAVNRGELLAVLGPNGAG
                     KSTAISILLGLQGADEGSAELFGHQPQEIEGRRRIGVMMQEVMLPGVMHPRELLEQVA
                     SYYPTPYDIDAVIKRMSLESLAQRPYGKLSGGQRRQVQFALAICGRPELLFLDEPTVG
                     LDVQAREALWKVVRELKHEGCSIVLTTHYLEEAEALADRVAVMARGRLIAGGSVNEIR
                     AHVTRKQVSCVTRLTADTVSTWPEVVQVELERDRLKITTRDAEALLMHLFREDPQLSD
                     IDVKRAGLAEAFTELTNDSKKEAA"
     gene            complement(1187..2236)
                     /locus_tag="VEW08_07850"
     CDS             complement(1187..2236)
                     /locus_tag="VEW08_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40687.1"
                     /translation="MDHTRVLLVPFHPTNLLMVGIFSVLLTFCLSAGFYGSLAALFLQ
                     IWVFKYCYVLIEHLADGASEPPVMDTDMLSPFEVRPWIQAAILFYGAWICWWIGGVTG
                     IVLGIALLAVFPATVAILGFGERPWQAVNPVIWYRVIRGLGPYYVMLLLALVVIAGIA
                     ALLNRLSLWSIIETSIALWCEVAFFCLVGAALFVRRRQLGYEPSRSPERAAARVEMER
                     IKERAKMVDDVFQLVRIGKHVDATAPLARWLNGTDPEHISKDAYFVAEQALRWESELA
                     LNTLGSTLIRHLMRYGRPDAALAVFEILRKRAPNFTMDSVTDLRTLADFAETEGREAL
                     AQSMRLETPVIHPQK"
BASE COUNT          457 a          709 c          738 g          415 t
ORIGIN      
        1 tacgccgcct ggccactgtt gccacgcatg cttccgaaaa agatgccgaa catcacatag
       61 aagagaatcg gaaataatag cgtcggcacg gcgaaagccg gcgtgcgcag tgacttgatg
      121 aactcgaagc gcatctcgcc gaggtacgcg gcccacaggc gcgagcctga catgttgctg
      181 acggcgttca tgcagcctcc ttcttggagt cgttggtgag ctcggtgaac gcttcggcga
      241 gcccggcgcg tttgacgtcg atgtcggaaa gttggggatc ctcgcggaac aggtgcatga
      301 gcaacgcttc ggcgtcgcgg gtggtgatct tcaggcggtc acgctccaat tccacctgga
      361 cgacctcggg ccacgtgctc acggtgtcgg cggtgagtcg ggtcacgcag ctcacctgtt
      421 tgcgcgtcac atgcgcgcgg atttcgttga cgctgcctcc ggcgatgagc cggccccgcg
      481 ccatgacggc gacccgatcg gcgagcgcct cggcttcttc gagatagtgc gtggtgagca
      541 cgatggagca gccttcatgc ttgagctcgc gcaccacttt ccagagcgcc tcgcgcgcct
      601 ggacgtcgag acccacggtg ggttcatcga ggaacagcaa ttcgggacgg ccgcagatcg
      661 ccagcgcaaa ctgcacctgg cgcctctggc cgccggacag cttgccgtag ggacgctgcg
      721 ccaggctctc gagcgacatt cgcttgatca ccgcgtcgat gtcatagggc gtggggtaat
      781 aacttgcgac ctgctcgagc agctcgcgcg gatgcatcac gccgggcaac atcacttcct
      841 gcatcatcac gccaatgcgg cggcggccct cgatttcctg cggctgatgg ccaaacaact
      901 ccgccgaacc ttcatcggca ccctgcaggc ccagcaggat ggagatggcg gtggatttgc
      961 cggcaccgtt cgggccgagc accgcgagca gctcgccgcg gttcaccgcg agatcgaagc
     1021 catcgagtgc ctggatcttt ccgaagcgct tgcgtacgcc cttgaggctt gcgagtgcgg
     1081 tttgcgtcat gtcccctcca ttgagcagat catggcgcgc cgggagcgcg aagctaagtc
     1141 gtccctgtca gccggccccg gtgacaactg tcatgtgtcg agatcgtcat ttttgcgggt
     1201 gaatgacggg tgtttccagg cgcatcgact gggcgagcgc ttcgcgacct tcggtctcgg
     1261 caaaatccgc gagcgtgcgc aggtcggtga ccgaatccat ggtgaagttc ggcgcccgct
     1321 tgcgcaggat ttcgaatacc gccaatgcgg catccggccg gccatatcgc atcaggtggc
     1381 gaatcagtgt gctgcccagc gtgttcagcg cgagctcgct ttcccagcgc agcgcctgct
     1441 cggcaacgaa gtacgcatcc ttggagatat gttccggatc cgtgccgttc aaccagcgcg
     1501 cgagcggcgc ggtcgcatcg acgtgtttgc cgatccgcac gagctggaaa acgtcgtcga
     1561 ccattttcgc tcgttccttg atccgctcca tttcgactcg cgccgcggcg cgctcgggac
     1621 ttcggctggg ctcgtagccg agctgacggc gccgcacgaa caacgcggca ccgaccaggc
     1681 agaaaaaggc cacctcgcac cagagcgcga tcgaggtttc gatgatggac cagaggctca
     1741 gccgattgag cagagcggcg atgccagcga tgaccacgag ggccagcagc agcatcacgt
     1801 aatagggacc gaggccgcga atgacgcgat accagatcac tgggttgacc gcctgccatg
     1861 gccgttcgcc aaagccgagg atggccacgg tcgcaggaaa taccgccagc agcgcgatgc
     1921 cgagaacgat gccggtcacg ccgccgatcc accagcaaat ccacgcgccg taaaacagga
     1981 tggcggcctg gatccacggg cggacctcga acgggctcag catgtccgtg tccatgaccg
     2041 gcggctcgga ggcgccgtca gcgaggtgtt cgatcaatac ataacaatat ttgaataccc
     2101 agatctgcag gaacagcgcc gcgagggacc cataaaatcc cgccgacagg cagaacgtga
     2161 gcaggacgga gaagattccg accatgagca ggttggtcgg atggaacgga accagaagaa
     2221 cgcgtgtgtg gtccataatg cggttctact cgggggttga agtaaagcat tgaaacgaat
     2281 tctttcgatg cgatcgtcgt gggatccgga atgagcggc
//
LOCUS       DASQJS010000191         8067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2305972, whole genome shotgun sequence.
ACCESSION   DASQJS010000191 DASQJS010000000
VERSION     DASQJS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2305972"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VEW08_07855"
     CDS             <1..376
                     /locus_tag="VEW08_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005400412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HYJ40688.1"
                     /translation="PVLRDVDLQGYADIEKNIGAFATRARDGKITIEELTGGTFSITN
                     GGTFGSLLSTPIVNMPQSAILGMHATKERPMVVNGQIEIRPMMYVALSYDHRIIDGRE
                     AVQFLVTIKDCLEDPARLLLSV"
     gene            400..1827
                     /gene="lpdA"
                     /locus_tag="VEW08_07860"
     CDS             400..1827
                     /gene="lpdA"
                     /locus_tag="VEW08_07860"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HYJ40689.1"
                     /translation="MSTEYDVIVIGGGPAGYPAAIRAAQNKLKVACIDEWKNKDGTAV
                     FGGTCTNAGCIPSKALLESSELYHKTKEELGVHGIKVGDVQFDVAQMQKRKTGIVKAS
                     TTGIAMLLKSAGVTALQGHGKLLAGRKVEFTAHDGKKETLSARHIVLASGSIPTELKS
                     LPFDGKNIVDSWGALDFETVPKKLAVVGAGVIGLELGSVWRRLGSEVVVLEALDTFLP
                     IADQGLAKEAARHFKKQGLDIKLGAKVSGAKASGNSVEVSYADASGSQTVTVDKVIVA
                     IGRKPFSKNLLADGTGVQIDERGFIKVDDHCKTGVENVWAVGDCVRGAMLAHKAKDEG
                     VMVGDMIAGRYGHMNYNTIPSVIYTHPEIAWVGLTEEQVKASGRAYKIGQFPFAASGR
                     ARAMEATAGFVKLIAAQDDDEILGVHIIGPMAGELIAEAVLAMEYSASSEDLQRTIHA
                     HPTLSEALHEAALAADKRAIDNINK"
     gene            complement(1872..3758)
                     /locus_tag="VEW08_07865"
     CDS             complement(1872..3758)
                     /locus_tag="VEW08_07865"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYJ40690.1"
                     /translation="MRVLLVDHDSEALETIARAIRGVLELDCVSSKGDALLLLRQNTY
                     DVLIACERAVDGSGLDLLGRTTRTAVPLKRIFAAAPERLQLLGPRLAPFKVQRTINYP
                     IDLEELWLAIAQVTGGPNDETDGTIERVVLDERGIPASGTTPRAPIPPRPPAPLGGVR
                     STPAPAPSPPPTPAIAASASGARAATARAVQPAPEPPLRAPPPPRQPPPAMRHAAATA
                     QMRAQAPAMKPIPPMPLPVAAPLTKAPVDDWTPEPPLEDDFAQVAAQARMGVQRKVVD
                     EVSRRKKQRLLTACGVAVAVAGVIVLLIEKFYDPEARARERAIAQEVTRMAEQQKVTD
                     NLTLIEVDIENAIMNNEVDQARTLLATLVEKSPDHPRREFLQDAIDRAAELQKLAGRP
                     PSQAQVMTPPTSAERKAASRPKPAERSAERTPSRTPDRVATRPPDRPVSPRSPSDTSP
                     TASRTYGAPISEPPPQSSIPLDAPINSTTTAPMRRTDNFPGRTREASDSAGGSAPASV
                     PSPGSSNESGSATVAMPPAAAPVQAPPAPVDVVPARIVKRVTPVVSADVPRKASGFVI
                     VKFEIGANGRVSDVAVVESSPAGVFDEAATTAVRKWTYEPRRENGVAVSSQSKARLVF
                     EAAN"
     gene            complement(3880..5859)
                     /locus_tag="VEW08_07870"
     CDS             complement(3880..5859)
                     /locus_tag="VEW08_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYJ40691.1"
                     /translation="MHNLHTCSAELRHSIDAHASRLESWGVPRAAIDKAVEAAVIVAA
                     MTEDPVLAAALMAQAGFASAASEAREIEQHFGPGVSSLAHELTHFGDIRLTPMAAAAH
                     HLEPAQAEALRKMLLSVVSDPRLVLARLARQLVDLNGSKDGHAATRERLALETREVFA
                     PLANRLGLWQLKWELEDLAFRFLQPDDYRRLADALAEKRADRERYIIELCRTLESMMH
                     TAGIDGEVYGRPKHIYSIHRKMERKQLSFEQVFDLRAVRIVTARIEDCYAVLGLVHGR
                     FPYIPGEFDDYIATPKDNFYRSIHTAVFGPEGKPVEVQIRTREMHDQAELGLAAHWKY
                     KEGRYKDARALDVTYDRKIEWVRKLLDSSERFAADGDRGFLDEVRAELFEDRVYALTP
                     KGEVVDLPPGATPLDFAYHVHTDLGHRCRGARVNGRIVPLTRALANGEIVEIITHKHP
                     QPSRDWLSETAGFLVSPRSRSKVRTWFRKLDEEAGTLPVVAPAPAPPPAPPRRLRASR
                     KPSRSPVTLGGVEDLPTSFARCCTPVKPEPIAGYATLSRGVTIHRADCPGLTRMSASH
                     PDRLIAAEWADAASSQPVTLAIVAIDRQALVRDISDVLASEKISIEMMHTTTDRTQST
                     ATIDLRVAIADAQHLTRLLQRIRKVTSVLSVRRSG"
     gene            5968..6543
                     /gene="ruvC"
                     /locus_tag="VEW08_07875"
     CDS             5968..6543
                     /gene="ruvC"
                     /locus_tag="VEW08_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HYJ40692.1"
                     /translation="MRSSWRAYQPGAEAPAGPAVARMLPGKPARILGLDPGSLRTGYG
                     VIDCAAGTLTIVAQGCIATSGGPLADRLRIIHAKVTELIGLHRPQEVAVERVFLSKNA
                     DSALKLGQARGAALAAVPASLGVHEYAPRAIKLAVVGVGGAEKAQVAHMVKQLLRLEM
                     KLGADAADALAIAICHANTRRLEPLFEGLGA"
     gene            6540..7139
                     /gene="ruvA"
                     /locus_tag="VEW08_07880"
     CDS             6540..7139
                     /gene="ruvA"
                     /locus_tag="VEW08_07880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HYJ40693.1"
                     /translation="MIGFVRGKLVFKAPPHLTVDVGGVGYDIEAPMSTFYTLPAVGSE
                     VRLLTHLTVREDAHILYGFGTADERVLFRNLLKVSGVGPKIALAILSGVSVDGFATCV
                     KLKDAAALTRIPGVGRKTAERLLVEMPDRLDALGAAVAAGAPVPNGENRTEGEAWSAL
                     VALGYKPAEVTQMLKPFAGVASSTEDLIRRALQGTVRPQ"
     gene            7136..>8067
                     /gene="ruvB"
                     /locus_tag="VEW08_07885"
     CDS             7136..>8067
                     /gene="ruvB"
                     /locus_tag="VEW08_07885"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HYJ40694.1"
                     /translation="MTAENIPERVVQAAGSTEEEAVDRAIRPKRLADYVGQEQVKAQL
                     EIFVKAAVMRGEALDHALIFGPPGLGKTTLANILASELGVNLRQTSGPVLERPGDLAA
                     LLTNLQPRDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKLALQPFTL
                     IGATTRAGLLTSPLRDRFGIVQRLEFYTVAELEKIVTRSAGILGVPIDVEGAARIAQR
                     SRGTPRITNRLLRRVRDYAEVKGTGRIDEAVARAALDLLEVDPQGFDALDRKLLSTII
                     EKFDGGPVGIESLSAAVGEERGTLEDVIEPFLIQ"
BASE COUNT         1403 a         2542 c         2736 g         1386 t
ORIGIN      
        1 tcccgtgctg cgcgacgtgg atctgcaggg ctacgccgac atcgagaaga acatcggcgc
       61 gttcgccacg cgcgctcgtg atggcaagat caccatcgaa gagctcaccg gcggcacgtt
      121 ctccatcacc aacggcggca ccttcggttc gctgttgtcc acgcccatcg tcaacatgcc
      181 gcagagcgcg atccttggca tgcacgccac caaggaacgc ccgatggtgg tcaatggcca
      241 gatcgagatc cgcccgatga tgtacgtggc gctcagctac gaccatcgca tcatcgacgg
      301 ccgcgaggcg gtgcagttcc tcgtgaccat caaggattgt ctcgaagacc cggcgcgcct
      361 gctgctgagc gtctgaagaa ataacagtta ggggttcgca tgagtactga gtacgacgtc
      421 atcgtcattg gtggcggtcc ggcgggttat cccgcggcca tccgcgcagc gcagaacaag
      481 ctcaaggtcg cctgcatcga cgaatggaag aacaaggacg gcacggccgt gttcggtggc
      541 acctgcacga acgcgggctg cataccctcc aaggcgttgc tggaatccag tgagctctat
      601 cacaagacga aagaggagct cggcgtccac ggcatcaagg tcggtgacgt gcagttcgat
      661 gtcgcgcaga tgcagaagcg caagaccggc atcgtgaagg ccagcaccac gggcattgcc
      721 atgctgctca agagcgcagg cgtcaccgcg ctgcaggggc acggcaaact actggcgggg
      781 cgcaaggtgg aattcaccgc gcacgacggc aagaaggaaa cgctgtccgc caggcacatc
      841 gtgctggcgt cggggtccat tcccaccgaa ctcaagagcc tgccgttcga cggcaagaac
      901 atcgtcgatt cctggggcgc gctcgatttc gaaaccgtgc cgaagaagct cgccgtggtc
      961 ggcgccgggg tcatcggcct cgagctcggc agcgtctggc ggcgtctcgg atctgaagtc
     1021 gtggtgctcg aagcgctcga cacatttctg ccgatcgccg accagggcct ggcgaaggaa
     1081 gccgcgcgac atttcaagaa gcaggggctc gacatcaagc tcggcgccaa ggtttcaggc
     1141 gccaaggcat ccggcaactc ggtcgaggtc agctacgccg acgcatcggg tagtcagacg
     1201 gtcaccgtag acaaggtcat cgtcgccatc ggccgcaagc cgttcagcaa gaacctgctg
     1261 gccgacggca cgggcgtgca gatcgatgaa cgaggattca tcaaggtgga tgatcactgc
     1321 aagacgggcg tcgagaacgt ctgggccgtg ggcgactgcg tgcgcggcgc gatgctcgcg
     1381 cacaaggcca aggacgaggg cgtgatggtg ggcgacatga tcgccggccg ttacggccac
     1441 atgaactaca acaccattcc ctcggtgatc tacacgcatc cggaaatcgc ctgggtcggg
     1501 ttgaccgagg aacaggtgaa ggccagcgga cgtgcgtaca agatcgggca gtttccgttc
     1561 gccgccagcg gccgcgcgcg tgcgatggaa gccaccgccg ggttcgtgaa gctgatcgcc
     1621 gcgcaggatg acgatgaaat cctgggcgtg cacatcatcg gccccatggc aggcgagctc
     1681 atcgccgaag ccgtgctggc gatggagtat tcggcaagca gcgaagatct gcagcgcacg
     1741 atccacgcgc atccgacgct gtccgaagcg ctgcatgaag cggctctggc tgccgacaag
     1801 cgcgccatcg acaacatcaa caagtagcca cgcaacgcgc agcaaccggg caacggattg
     1861 ccgccggcta gctagttggc cgcctcgaat accaggcggg ccttggactg ggaactcacg
     1921 gccacgccgt tttccctgcg cggttcgtag gtccacttgc gtacggccgt cgtggctgcc
     1981 tcgtcgaata cgccggccgg cgacgattcg accacggcga cgtcactgac acgtccattc
     2041 gcaccgatct cgaacttcac gatgacaaag ccggatgcct tgcgcggcac gtcggccgaa
     2101 accaccggcg tcacgcgctt cacgatcctg gcgggaacga catcgacggg cgcaggcggc
     2161 gcctgtaccg gcgcggcggc gggcggcatg gccacggtgg cggaacctga ttcgttcgaa
     2221 ctaccgggag atgggaccga cgcgggggca ctgcccccgg cagagtcgct ggcttccctg
     2281 gttcgacccg ggaaattgtc ggtgcgacgc atgggcgccg tggtggtcga attgatgggc
     2341 gcgtcgagcg gaatgctcga ctggggcggc ggttcgctga tgggggcgcc gtaggtacgt
     2401 gacgccgtgg gcgatgtatc gctcggggaa cgtggcgaaa ccgggcggtc cggcggccgc
     2461 gtggcaacgc ggtcgggagt gcgcgaaggc gtgcgctcgg ccgagcgctc cgcgggtttg
     2521 ggtcgcgagg cggccttgcg ctcggcggaa gtgggtggcg tcatcacctg ggcctgcgac
     2581 gggggccggc ctgccagctt ctgcagctcg gcggcgcggt cgatggcgtc ctgcagaaac
     2641 tcgcggcgcg gatggtcggg tgatttttca acgagcgtgg cgagcagcgt gcgcgcctga
     2701 tcgacctcgt tgttcatgat cgcattctcg atgtcgactt cgatcagcgt caggttgtcg
     2761 gtgaccttct gctgctccgc catccgcgtg acttcctggg caatggcccg ttcgcgggcg
     2821 cgcgcctcgg ggtcgtagaa cttctcgatg aggagcacga tgacgccggc gacggccacg
     2881 gccacaccgc aggccgtgag caggcgctgc ttcttgcggc gcgagacttc gtcgaccact
     2941 ttgcgttgca cgcccatacg cgcctgcgcg gcaacctggg cgaagtcgtc ttcgagcggc
     3001 ggttcgggcg tccagtcatc gactggtgct ttcgtcagcg gtgccgcgac cggcaagggc
     3061 atcggcggaa tgggcttcat cgccggtgcc tgagcccgca tctgcgcagt ggctgccgcg
     3121 tggcgcatgg ccggtggcgg ctggcgcggc ggaggtggcg cgcgcagcgg aggttcagga
     3181 gccggttgaa cggctctcgc ggtggcggcg cgggcgccgc ttgccgatgc ggcgatggcg
     3241 ggtgtcggcg gcggcgaggg cgcaggtgcc ggcgtagagc gcacgccgcc gagtggcgct
     3301 ggcggacgtg gcgggatcgg tgcgcgcggc gtagtaccac ttgcagggat gccgcgctcg
     3361 tcgagcacga cacgttcaat ggttccgtcg gtttcgtcgt tggggccgcc cgtgacctgc
     3421 gcgatggcca gccacagttc ttcgagatcg atcggatagt tgatggtccg ctgcaccttg
     3481 aacggtgcaa gccgcggacc gagcagctga aggcgctcgg gagcggcggc gaaaatgcgc
     3541 ttgagcggaa cggcggtgcg ggtagttcgg ccgagcaggt cgaggcccga gccatcgacc
     3601 gcgcgctcgc aggcgatcag cacgtcgtag gtgttctgac gcagcagcag caacgcgtca
     3661 cctttgctgg agacgcagtc gagctcgagc actccgcgaa ttgcacgcgc aatggtctcg
     3721 agtgcctctg agtcatgatc gaccagcagg acgcgcatga gcgttctgaa accccggata
     3781 aatccaataa atcgttatga gtaaacgcga ttcgacgaaa tcgggcgtcg cagcacgtca
     3841 cgtttcgatg gcccccaggg gccgttttcc gggcttaagt taacccgaac gacggaccga
     3901 aaggacgctc gtcaccttcc tgatgcgttg tagcaaacgc gtcagatgct gcgcgtcagc
     3961 aatcgccacg cgcaggtcga tggtagctgt cgactgcgta cgatcggtag tggtgtgcat
     4021 catctcgatg ctgatttttt cactcgcgag cacatcgcta atgtcccgga ccaaggcctg
     4081 tctgtctatg gcgacaatcg ccagcgttac cggctggctg gaagccgcat cggcccattc
     4141 cgcggctatc agtcgatcgg gatggcttgc actcatgcga gtcagtcccg ggcaatccgc
     4201 ccgatggatg gtcacgccgc gactcaaagt ggcgtagccg gcgatgggtt cgggcttcac
     4261 cggtgtgcaa caccgcgcaa agctggtcgg caggtcttcg acaccgccga gggtgaccgg
     4321 cgagcggctg ggtttacgcg aggcgcgcag gcgtcgcggt ggtgccggcg ggggtgccgg
     4381 ggcgggcgcc accacgggca gcgtgcccgc ttcctcgtct aacttccgga accaggtacg
     4441 taccttggag cggctgcgtg gcgaaaccag gaagcccgca gtctccgaga gccagtcccg
     4501 gctcggctga ggatgcttgt gagtgatgat ctcgacgatt tcgccattcg ccagggcgcg
     4561 ggtcagcggc acgatgcgcc cgttcacgcg cgcgccacgg cagcggtgtc ccaggtccgt
     4621 atgcacgtgg tacgcaaaat cgagcggcgt ggcgcccggt ggcaggtcga cgacttcgcc
     4681 cttgggcgtg agcgcataga cgcgatcctc gaacagctcg gcgcggacct cgtcgaggaa
     4741 accgcgatcg ccatccgcgg caaatcgttc ggatgagtcg agcagtttac ggacccactc
     4801 gatcttgcgg tcgtaggtga catcgagggc gcgcgcgtcc ttgtagcgcc cctctttgta
     4861 tttccaatgg gctgccagcc cgagctctgc ctggtcgtgc atctcgcgcg tgcggatctg
     4921 cacctccacc ggtttaccct cggggccgaa cacggcggta tggatggacc ggtagaaatt
     4981 gtctttcggc gtggcgatgt agtcgtcgaa ctcgccgggg atgtagggaa atcgtccgtg
     5041 aaccagcccg agcacggcgt agcagtcttc gattcgcgca gtcacgatgc gcaccgcgcg
     5101 caggtcgaac acctgctcga acgagagctg cttgcgctcc atcttccggt ggatgctgta
     5161 gatgtgcttg gggcgtccgt acacctcgcc gtcgatgccg gcggtgtgca tcatcgattc
     5221 gagcgtgcgg cacaactcga tgatgtagcg ttcgcggtcg gcgcgcttct cggcaagcgc
     5281 gtcggcgagg cgccggtagt catcgggctg caaaaagcgg aaagccaggt cctccagctc
     5341 ccatttgagc tgccacagcc ccaggcgatt ggcgagcggc gcgaacacct cgcgcgtctc
     5401 gagcgccagc ctttcacgcg tggcggcgtg gccatctttc gagccgttga gatcgaccag
     5461 ttgccgcgcg agtcgcgcca gcacgaggcg cggatcgctc accaccgaca gcagcatctt
     5521 gcgcaacgcc tcggcctgcg ccggctcgag gtggtgtgcg gccgcggcca tgggcgtcag
     5581 ccggatgtcg ccaaagtgcg tgagttcgtg cgccagcgaa ctcacgcctg gcccgaaatg
     5641 ctgctcgatc tcgcgtgctt ccgaggccgc cgaagcaaac cccgcctgcg ccatcagcgc
     5701 ggccgcaagc accggatcct cggtcattgc ggcgacgatg acggccgctt cgacggcctt
     5761 gtcgatggct gcgcggggca cgccccagct ttcgagccgg gatgcgtgcg cgtcgattga
     5821 atgccgcaat tccgcggaac aggtgtgaag gttgtgcacg ggattagcag gtaaccggcg
     5881 cgtgaggcgc agcggatttg cggctatgat acgcgcagtt tttctcaagc agcacagagc
     5941 tttaagcgac tgatggcgcg atacccgatg agatcatcct ggcgcgcata ccagcccggc
     6001 gcggaagccc cggcaggtcc tgcggtcgcg cggatgttgc cgggcaaacc tgcgcgcatc
     6061 ctggggctcg atcctggctc gcttcgtacc ggttacggcg tcatcgattg tgccgccggc
     6121 actctcacca tcgtcgcgca gggctgtatc gcgacgtcgg gtgggccgct cgcggatcgt
     6181 ctgcgcatca tccacgcgaa ggtcaccgaa ctcatcggcc tgcatcgccc gcaggaagtc
     6241 gccgtcgaac gcgtgttcct ttccaagaac gccgacagcg cgctcaagct cggtcaggcg
     6301 cgcggtgcgg cgctcgcagc cgtgcccgcg tcgctgggtg ttcacgaata cgcgcctcgc
     6361 gcgatcaagc tcgccgtggt gggcgtgggc ggcgccgaaa aggcgcaggt cgcgcacatg
     6421 gtcaaacaac tgctgcggct cgaaatgaaa ctcggcgccg atgcggctga tgcgctcgcc
     6481 atcgccattt gccatgcgaa cacgcgacgc ctcgagcctt tgttcgaagg acttggtgcg
     6541 tgatcggctt cgtccgcggg aaactcgtct tcaaggctcc gccgcatctc acggtggacg
     6601 tgggcggcgt cggctatgac atcgaagcgc cgatgtccac gttctacacg ctgcccgcgg
     6661 tcggaagcga agtgaggctg ctcacgcatc tgacggtgcg cgaagacgcg cacatcctct
     6721 acggcttcgg tacggcggat gaacgcgtgc tgttccgcaa cctgctcaag gtgtccggag
     6781 tggggcccaa gatcgcgctc gccatcctgt cgggcgtcag cgtcgatggc ttcgcgacct
     6841 gcgtgaagct caaggacgcc gccgccctca ccaggattcc cggcgtcggc cgcaagaccg
     6901 ccgagcggtt gttagtcgag atgcccgatc gcctcgatgc actcggcgcc gcggtggccg
     6961 ccggcgcacc cgtgccgaac ggcgagaacc gcaccgaagg tgaggcctgg agcgcactcg
     7021 tcgcgctggg ttacaagccc gccgaggtca cgcagatgct caagccgttc gccggcgtcg
     7081 ccagctctac cgaggacctg atccggcgtg cgctccaggg cacggtgcgt ccgcagtgac
     7141 cgctgaaaac attcccgagc gcgtcgttca ggcggcgggt tccaccgaag aagaggcggt
     7201 cgatcgcgcc atccggccca agcgcctcgc ggactatgtc ggccaggaac aggtcaaggc
     7261 gcagctggaa attttcgtga aggcggcggt catgcgcggc gaagcgctcg atcacgcgct
     7321 gatcttcggg cctcccggtc tcggcaagac cacgctcgcc aacattctcg ccagcgagct
     7381 cggcgtgaat ctgcggcaga cttccggccc tgtgctggag cgtcccggcg atctcgccgc
     7441 gttgctgacc aaccttcagc cgcgcgacgt gttgttcatc gacgaaattc accggctctc
     7501 gccggtggtc gaagaagtgc tctatccggc gatggaggac taccagctcg acatcatgat
     7561 cggtgaaggc ccggccgcgc gttccatcaa gctcgcgttg cagccgttca cgctcatcgg
     7621 cgcaaccacg cgtgcgggtt tgttgacctc gccgttgcga gaccgattcg gcatcgtgca
     7681 gagactcgag ttctacaccg tcgccgagct ggagaagatc gtcactcgtt cggccggcat
     7741 cctcggggtg cccatcgatg tcgaaggcgc cgcgcgaatc gcgcaaagat cgcgtggcac
     7801 gccgcgcatc acgaaccggc tgttgcgccg ggtgcgcgac tacgccgaag tgaagggcac
     7861 ggggcgcatc gacgaagccg tggcgcgcgc cgcgctcgat ctgctggaag tcgacccgca
     7921 gggcttcgac gcactcgacc gcaaactact ctcgaccatc atcgagaaat tcgacggtgg
     7981 tccggtgggc atcgaaagcc tgtccgccgc ggtgggcgaa gagcgcggca cgctcgaaga
     8041 cgtgatcgag cccttcctca tccagca
//
LOCUS       DASQJS010000192         6114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1047301, whole genome shotgun sequence.
ACCESSION   DASQJS010000192 DASQJS010000000
VERSION     DASQJS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047301"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VEW08_07890"
     CDS             <1..1307
                     /locus_tag="VEW08_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYJ40695.1"
                     /translation="VFDLNDQDVFWCTADIGWVTGHSYVAYGPLAAGATIVLYEGAPT
                     TPHPGRFWQICESLGVTIFYTAPTAIRALMKLGDDIPARYDLTRLRLLGSVGEPINPE
                     AWMWYHEHVGRMRCPIVDTWWQTETGAIMISPLPGTTATKPGSCTQPLPGIDATIVDA
                     RGEPVEGADAGGYLVIRKPWPSMLRTIWGDNERYLKTYWETFQNRYYVAGDSAHRDKD
                     GFFWIMGRIDDVLNVAGHRLGTMEIESALVAHPKVAEAAVVGKQHEIKGESVFAFVVL
                     RGDRPTGDKIEAFTKTLRDWVTEQLGAIARPDDIRFSDNLPKTRSGKIMRRLLRAIAR
                     NEDITQDLSTLENPAIIEQLRGGETKKVVAPRKAAPTKKAALKKKAAPARKAAPKKKP
                     APRKKAAPKKKVVARKKAVAKKKGPPARKASRKARGKPRGKK"
     gene            complement(1311..1970)
                     /locus_tag="VEW08_07895"
     CDS             complement(1311..1970)
                     /locus_tag="VEW08_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HYJ40696.1"
                     /translation="MSSPATSLSAPPYSRSEELAHALTAGLGIVACAIAIPWLAWVAA
                     GDALRLAGAMVFGTTALAMFTTSVIYHWEREPGRKQALRKLDHSAIYLLIAGTYTPFT
                     LVAMQGAWGWSLFGVVWTMAVLGVVAKTTVGFRYPRLSTLLYLGMGWLIVIAIKPLRE
                     NLSAAEFGWIAAGGLLYTGGVPFYVWKTKRYTHAVWHLFVLGGVACHFVAVLSLVGGR
                     D"
     gene            complement(2000..2746)
                     /locus_tag="VEW08_07900"
     CDS             complement(2000..2746)
                     /locus_tag="VEW08_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40697.1"
                     /translation="MKIFILGGSQLNARAVACLALGLAMAGCATDSRPVVVGQPMVVP
                     TLNDYCALAQKEIATSRVPARNVLVTDFQAFSRASPSVKPLETLQYVGYADPQRTRAR
                     MVSCKLHSAERIRAEYGATAAGESTTCARLNRRTLDAVMLSLTDRQKKKMPFKGTIGV
                     LLDPDEQATSETQWLESFTMVQTDAGGTLRIRAKSLGSGNMRVSSRANASNGRQYCHL
                     IAPDYLKRILIGEVELPKGEFPQSGQTASR"
     gene            2861..3937
                     /locus_tag="VEW08_07905"
     CDS             2861..3937
                     /locus_tag="VEW08_07905"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HYJ40698.1"
                     /translation="MGAVLMTESSPAADARPFVIAHRGASGYVPEHTLAAYFIAVQQG
                     ADYVEPDLVISRDGVLLARHENEIGGTTDVASHPEFAARKTTKTIDGESINGWFTEDF
                     TLAELKTLRARERLPGLRKANARYDGEFSIPTFDEVLDLVAAADAQRAEMARAAGLPA
                     PPRIGVYPETKHPSYFAKLGLTFDDRMLEALSRHGYSKRSDPICLQSFEVANLKALRR
                     RTDLSLVQLVAPTGQPFDFTLTGDKRSYLDLMTDSGLEEIAAYADAIGPHKWMVVQFG
                     AEGAGDTGLARRARAAGLGIHVWTLRAENEFLPEVFRSAGDAAAHGDLKMEIQALLDA
                     GITAFFSDHPDLAVRARDDWWARQ"
     gene            3955..4371
                     /locus_tag="VEW08_07910"
     CDS             3955..4371
                     /locus_tag="VEW08_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ40699.1"
                     /translation="MPQPFHLAIPVHSLSAARDFYGRLFGCPEGRSSDEWVDFDFFGH
                     QVVVHLDPGRKQHVHHNEVDGHDVPVPHFGVVLDWRAWHALAERLRSSGTRFEIEPGI
                     RFAGQVGEQATMFLYDPSGNALEFKAFKDPSQLFAK"
     gene            complement(4372..>6114)
                     /locus_tag="VEW08_07915"
     CDS             complement(4372..>6114)
                     /locus_tag="VEW08_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HYJ40700.1"
                     /translation="VIAHVVARRVVGGNTEISLGSNPDVTVDLLPEVAKLRAAGTPVV
                     MLGQVHREMPFMLGAASLPAEEFDLLLDHSRYDYDLFAPPNPSLSAVDHAIGLHASAL
                     VRDGGTLQIGIGELGDSIVYSLLLRHQQNEAWRRALIDAGTERNAGLIDSVGGRAPFA
                     TGLFGATEMFVDQMLDLYRAGILRRRVYDYLPLQRALAANGSVTRIDESLLEALLAAG
                     AGPALDANDVAALKAAGVLRSETRFEKGNIVSPDGVRIEADLGSASVRAALAQDCLGL
                     QLQGGTVMHAGFLLGPRSFYSALCALPENERRLFDMRGVGYINQLYGDDAALRVAQRG
                     HARFVNTTMMLTTLGAAVSDGLADGRVVSGVGGQYNFVAMAHALPGARSVLCVRSTRR
                     KDGKLASNIVTSYGHTTIPRHLRDIAVTEYGIADLRGRTDAECVAAMLKIADSRFQDA
                     LLADAKRANKIDAGYQIPEPHRHNTPQRLEEAFAVQRRAGLFSEYPFGTDLTREEIEL
                     ARALRWLKENTSGKVARLRTIARALFVKPRDSEGPCLARLQLDAPVDFPTRLTAKLVR
                     LSLRATADERPASP"
BASE COUNT         1157 a         1955 c         2005 g          997 t
ORIGIN      
        1 gggtgttcga tctcaacgac caggacgtgt tctggtgcac ggcggacatt ggctgggtca
       61 ccgggcacag ctacgtggcc tacggcccgc tggccgcggg cgccaccatc gtgctgtacg
      121 aaggtgcgcc aaccacgccg catccgggac gcttctggca gatctgcgag tcgctgggcg
      181 tcaccatttt ctacacggcg cccaccgcga tccgcgcgct catgaaactc ggcgacgaca
      241 taccggcgcg ctacgacctc acgcgcctgc gtttgctcgg gtccgtgggc gagcccatca
      301 accccgaagc ctggatgtgg taccacgagc acgtcgggcg catgcgctgc ccgatcgtcg
      361 acacgtggtg gcagaccgag acgggcgcca tcatgatttc gccgcttccg ggcaccaccg
      421 ccaccaagcc aggttcgtgc acgcagccgc tgcccggcat cgatgccact atcgttgatg
      481 cgcgcggtga gccggtcgaa ggcgccgacg ccggtggcta cctggttatc agaaaaccct
      541 ggccgtcgat gttgcgcacg atctggggcg acaacgaacg ttatctcaag acctactggg
      601 agacgtttca gaaccgctac tacgtggcgg gtgattccgc gcaccgcgac aaggatggct
      661 tcttctggat catgggccgc atcgacgacg tgctgaacgt cgccggtcac cggctcggca
      721 cgatggagat cgaatccgcg ctggtggcgc atccgaaggt ggccgaagcc gcggtggtcg
      781 gcaaacagca tgagatcaag ggtgagtcgg tattcgcatt cgtggtgttg cgcggcgatc
      841 gccccaccgg cgacaagatc gaagcgttca cgaagacgct gcgcgattgg gtgaccgagc
      901 agttaggcgc catcgcccgg cccgacgaca tccgcttctc cgacaatctg ccgaagaccc
      961 gctcgggcaa gatcatgcga cgcctgttgc gcgccatcgc gcgcaacgag gacatcacgc
     1021 aggacctgtc gacgctcgag aatccggcga tcatcgagca gctgcgcggt ggcgaaacga
     1081 agaaagtggt cgcgcccagg aaagccgcgc cgacgaagaa agccgcgctg aaaaagaaag
     1141 cggccccagc aagaaaggcg gcgccaaaga aaaagcccgc gccgcggaag aaggcggcgc
     1201 cgaagaagaa agtcgtcgca aggaaaaagg ccgttgcgaa gaagaaaggc ccgccggcaa
     1261 ggaaggcttc gcgcaaggcg agaggcaaac cgagaggaaa aaaatagccg tcagtcgcgt
     1321 ccgcccacca ggctcagcac cgcgacgaag tgacaggcga cgccacccag cacgaacagg
     1381 tgccacacgg cgtgtgtata acgctttgtc ttccagacgt agaacggcac cccgccggtg
     1441 tacagcaggc cgccggcggc gatccagccg aactccgcgg ccgagaggtt ctcgcgcagc
     1501 ggtttgatcg cgatgacgat gagccagccc atgccgaggt agaggagcgt cgagagacgc
     1561 ggataacgaa aacccaccgt ggtcttcgcc acgacgccga gcaccgccat ggtccagacc
     1621 acgccgaaca aggaccagcc ccaggcgccc tgcatggcca ccagcgtgaa cggcgtatag
     1681 gtgccggcga tgagtagata gatggcggag tgatcgagct tgcgcagcgc ctgcttgcgt
     1741 ccgggctcgc gttcccagtg gtagatcacc gaagtcgtga acattgcgag cgccgtggtg
     1801 ccgaacacca tcgcgccagc gagccgcaac gcatcaccgg cggcgaccca cgcgagccac
     1861 ggaatggcga tggcgcaggc cacgattccg aggcccgcgg tcaacgcgtg cgcgagttcc
     1921 tcgctgcggg aatagggtgg ggcagaaagt gatgtggcgg ggctggacac ccgccggagc
     1981 ttaacagctg ccggtgaacc taccggctcg cggtctgacc gctctgcggg aactcaccct
     2041 tcggcaattc cacttcgccg atgaggatgc gcttcaggta gtccggcgca atcaggtggc
     2101 aatactgccg gccattgctg gcgttggccc tggagctgac tcgcatgttg ccgctgccga
     2161 gactcttggc gcgaatgcgc agcgtgccgc cggcatcggt ctgcaccatg gtgaatgatt
     2221 ccagccactg cgtttcgctg gtcgcctgct cgtccggatc gagcagcacg ccgatggtgc
     2281 ccttgaacgg catcttcttt ttctggcggt cggtgagcga aagcatcacg gcatcaagcg
     2341 tgcgccgatt gagtcgggcg caggtggtcg attcaccggc ggcagtggcg ccgtattcgg
     2401 cgcgaatgcg ttcggcgctg tgcagcttgc acgagaccat acgcgccctg gtgcgttgcg
     2461 gatcggcgta gcccacgtat tgcagcgtct cgaggggctt caccgaaggt gatgctcgcg
     2521 agaacgcctg gaagtcggtg accagcacgt tgcgcgccgg cacacgggag gtggcgattt
     2581 ctttctgcgc gagggcgcag tagtcgttga gggtggggac gaccatcggc tggccgacga
     2641 cgaccgggcg cgagtcagtg gcgcatccgg ccatggcgag gccgagcgcg agacaagcca
     2701 cagcgcgggc attaagctga gacccgccaa ggatgaagat cttcatgggc gccactcccc
     2761 tgttgaaaaa acccagttgg aaaaacaatt ccgatgcagg tcgccagcga ccgaaagtcg
     2821 ccatcgcggg ccattctggc gttacgtcat atccggattc gtgggcgcag ttctcatgac
     2881 ggaaagctcg ccggccgccg atgcgcgtcc atttgtcata gcgcatcgcg gcgccagcgg
     2941 gtatgtgccg gaacacacac tcgccgcgta cttcattgcc gttcaacagg gtgcggatta
     3001 cgtcgaaccc gacctcgtga tatcccggga cggagtcctg ctcgcgcgcc acgagaacga
     3061 gatcggcggc acgaccgacg tggcatcgca cccggaattc gccgcacgca agaccacgaa
     3121 gaccatcgat ggggaatcca tcaacggctg gttcaccgaa gatttcacac tcgcggaact
     3181 gaagacgctg cgcgcgcgcg aacgcctgcc cggcctgcgc aaggcgaatg cgcgctacga
     3241 cggcgagttc tcgataccga ccttcgatga agtcctcgat ctcgtggcgg ccgccgacgc
     3301 gcagcgggcc gagatggcgc gcgccgccgg attgccagcg ccaccgcgaa tcggcgtcta
     3361 cccggaaacc aagcatccga gctatttcgc gaagctgggc ctgaccttcg acgatcggat
     3421 gctcgaagcg ctgagccgcc acggctacag caagcgatcg gatcccatct gcctgcagtc
     3481 cttcgaggtc gcaaacctca aggcgcttcg tcgccgcacc gatctgtccc tggtgcagct
     3541 ggtggcgccc accggacaac ccttcgattt cacgctgacg ggtgacaaac gcagttacct
     3601 cgatctcatg accgattcag gactcgaaga gatcgccgcc tacgcggacg ccatcgggcc
     3661 gcacaagtgg atggtggttc agttcggtgc ggaaggcgca ggagataccg gtcttgcccg
     3721 ccgggcgcgt gcggccggac tcggcattca cgtgtggacg ctccgcgccg agaacgaatt
     3781 tctaccggaa gttttccgat cggcgggcga tgctgccgcg catggcgacc tgaaaatgga
     3841 aatccaggcg ctgctcgacg ccgggatcac ggcattcttt tcggatcacc ccgaccttgc
     3901 cgtgcgtgcg cgcgacgact ggtgggccag gcaatgaacg ggagaagcta aagcatgccg
     3961 cagccatttc atctcgcgat cccggtgcac tcgttatccg ccgcgcgcga tttctacgga
     4021 aggttgttcg gttgcccgga agggcgcagc agcgatgaat gggtggattt cgatttcttc
     4081 ggccaccagg tcgtcgtgca cctggaccca ggcaggaagc agcacgtgca tcacaacgaa
     4141 gtcgacgggc atgacgtgcc cgtgcctcat ttcggcgtgg ttctcgactg gcgggcctgg
     4201 cacgcgctcg ccgagcgcct gcgcagctcg ggtacacgct tcgaaatcga gccgggcatc
     4261 cgcttcgcgg gccaggtagg tgagcaggcc acgatgttcc tctacgaccc atccggcaac
     4321 gcgctcgagt tcaaggcgtt caaggatcct tcgcagctct ttgcgaaata gctagggcga
     4381 agcaggccgt tcgtctgccg tggcgcgcag cgagaggcgc accagcttcg cggtcaagcg
     4441 cgtcggaaaa tcgacgggcg cgtcgagctg caggcgcgcg aggcacgggc cttcgctgtc
     4501 ccgcggtttc acgaacaatg cccgcgcgat cgttcgcagg cgcgcaactt tgccgctggt
     4561 attttccttc agccagcgca acgcgcgcgc cagttcgatt tcctcacgcg tgagatcggt
     4621 gccgaacgga tactccgaga acaagcccgc acggcgctgc acggcgaagg cttcttcgag
     4681 ccgttgcggg gtattgtgcc gatgcggctc gggtatctgg taaccggcgt cgatcttgtt
     4741 ggcgcgtttt gcatccgcga gcaacgcatc ctggaagcgc gaatccgcga ttttcaacat
     4801 ggcggccacg cactcggcgt cggtgcggcc gcgcagatcc gcgatgccgt attcggtgac
     4861 ggcgatgtcg cgaagatgac gcggaatcgt ggtgtgccca taactcgtca cgatgttgga
     4921 ggcgagcttg ccgtctttcc ggcgtgtcga gcgcacgcac agcaccgacc gcgcgcccgg
     4981 cagcgcgtgg gccatggcga cgaagttgta ttggccgccg acgcctgaaa ccacccggcc
     5041 atcggcgaga ccatcagaga ccgcggcacc gagcgtcgtc agcatcatcg tggtgttcac
     5101 gaaacgcgca tgaccgcgct gcgcaaccct caaggccgcg tcgtcgccgt agagttgatt
     5161 gatgtagccc acgccgcgca tgtcgaacag ccggcgttcg ttctccggca atgcgcacaa
     5221 cgccgaatag aaggagcgcg ggccgagcag gaacccggca tgcatcacgg tgccgccctg
     5281 cagctgaagc cccaggcagt cctgcgccag tgcggcgcgc acgctggcgc tgccgagatc
     5341 ggcctcgatg cgcacgccgt cgggcgaaac tatgttgccc ttctcgaagc gcgtttcgct
     5401 gcgcaataca ccggcggctt tgagtgccgc gacatcgttc gcatcgagcg ccgggcccgc
     5461 tcccgcggcc agcaaggcct cgagcaggga ttcgtcgatg cgcgtcacgg agccgttggc
     5521 ggccagcgcc cgctgcagcg gtagatagtc gtagacgcgg cggcggagaa ttccggcgcg
     5581 atacaggtcg agcatctggt cgacgaacat ttcggtggcg ccgaacaggc ccgtggcaaa
     5641 gggtgcgcgg ccgccgactg aatcgatgag ccccgcgttg cgctccgtgc ccgcatcgat
     5701 gagcgcgcgc cgccaggctt cgttctgctg gtgacgcagc agcagcgagt acacgatcga
     5761 gtcgccgagc tcgccaatgc cgatctgcag cgtgccgccg tcgcgcacca gggcgctcgc
     5821 gtgcaggccg atcgcatggt ccacggccga cagcgacggg ttcggcggcg cgaacaggtc
     5881 atagtcgtag cgcgaatggt cgagcaacag gtcgaattct tccgcgggca ggctggccgc
     5941 gcccagcatg aatggcatct cgcggtgcac ctggcccagc atcaccaccg gagtccccgc
     6001 ggcgcgcagc tttgcgacct ctggcagcag gtcgaccgtg acatccggat tgctgccgag
     6061 actgatctcg gtgttgccgc ccaccacgcg tctggccacg acgtgcgcga tgac
//
LOCUS       DASQJS010000193         8612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4187665, whole genome shotgun sequence.
ACCESSION   DASQJS010000193 DASQJS010000000
VERSION     DASQJS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4187665"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VEW08_07920"
     CDS             <1..888
                     /locus_tag="VEW08_07920"
                     /inference="COORDINATES: protein motif:HMM:NF019944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HYJ40701.1"
                     /translation="AQVVAQVDMTTTESTREQFNPQSQVVRSESTAEEAARNGAGNAG
                     GVPGALTNQPPQPGVALPPGATPAVAQSGSNSAGASAAGTKPNNADGTVVTGPDSTSR
                     QLTRNYEIDRTMAYTRAPAGRVTRLSVAVLIDNLRTTAADGKVTESPLPPEQLERLTA
                     LVRDAVGFDATRGDTVNVVNSSFLGTPVIEEGELESIPFWERAWAQTLAKVLAGLLVL
                     LVIVFSVLKPLTKGLLTAARPSVLRQAALAAANVGGGGPAEAPALAYEQQVAAARGMV
                     AQDPKRGAQVVKAWVQSDA"
     gene            881..1894
                     /gene="fliG"
                     /locus_tag="VEW08_07925"
     CDS             881..1894
                     /gene="fliG"
                     /locus_tag="VEW08_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HYJ40702.1"
                     /translation="MPEATKTRTGVERAAILLLTLGESEAAEILKHMGAKDVQRLGRS
                     MAELTSVSRDEVRDVLNKFTSQLEDQTSLGVGSDDFVRKVLVNALGDEKANSLMDRIR
                     LGGQRKGLEALKWMDSRAVAELIRNEHPQIIAIVLSYLEADQGAEVLGFLPENARSDL
                     IVRVATLDGVQPSALTELDEMMEKQFAAGASNGKSSSLGGLKVAAEMVNLLDAKMGSA
                     VMTEVGKTDERLSQSIQDLMFVFDDLLEIDDRGMQELLRQVPADKLLLSLKGADETFK
                     AKIFKNMSQRAAEMMKDDLANKGPVRLADVEAAQKEILLAARKLADDGTINLGGKGGD
                     EYV"
     gene            1887..2552
                     /locus_tag="VEW08_07930"
     CDS             1887..2552
                     /locus_tag="VEW08_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HYJ40703.1"
                     /translation="MSEPLARWDLPKVEGASLPRGSGGVNVMHLAAVERDAWEHGYKA
                     GHVEGVRKGEAELKVRIAEVDVKVTALQAIIDTLAKPLDDLDTGIETELTRLAMIIAK
                     HLVRRELRLDPAQVIGIVRHTVGLLPLAARNIKVHLHPDDAAILRDRLAAPVGEREWD
                     LREDPLMARGGCRVTTDNASIDARVESTIAAAMRGLLGDDRAERVEAAVAAADPPADE
                     DPA"
     gene            2549..3937
                     /gene="fliI"
                     /locus_tag="VEW08_07935"
     CDS             2549..3937
                     /gene="fliI"
                     /locus_tag="VEW08_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HYJ40704.1"
                     /translation="MSATNEVRTERSRTWRKSIDMQVARAANLPSPPVEGSLTRMVGL
                     ALEASGCQAAVGDVCDLISSDGSRCEAEVVGFSGDKLLMMPTGDVHGLAPSARVIPRQ
                     RAGSVRVGPGLLGRIIDGTGAPLDGLGPLNGDERVKLQGKSINPLERHPIDSPLDVGV
                     RSINSLLTVGRGQRIGLFAGSGVGKSVLLGMMARYTSADVIVVGLIGERGREVKEFVD
                     RILGPEGRRRSVVVASPADNPPLIRLHGAWLATSIAEYFRERGLSVLLIMDSLTRFAQ
                     AQREIGLAIGEAPATRGYPPSVFARLPALVERAGNGSTGRGSITAFYTVLTEGDDQQD
                     PIADAARAILDGHVVLSRRIAEAGQYPAIDIEASVSRVMNEIVSPEHAELARRFRQNL
                     AVYQQHRDLISIGAYQKGSDPRVDTAIELWPAMQKFLMQPIEERVGQRDSVQALAQLF
                     ASAPPPPRKAGT"
     gene            3934..4383
                     /gene="fliJ"
                     /locus_tag="VEW08_07940"
     CDS             3934..4383
                     /gene="fliJ"
                     /locus_tag="VEW08_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HYJ40705.1"
                     /translation="MTRSERLDVVKQATAHNEREHAERVAAAERHVVEMEEKLQALER
                     YRKEYEDGFAARAGAGLGVAAMRDFQAFLARLGDALEQQRQVVEQARAAHQATILVWR
                     EAAKRAHVVDTLAERWQIEESREEGRRDQRDNDELSQQMRFNRERKT"
     gene            4380..5372
                     /locus_tag="VEW08_07945"
     CDS             4380..5372
                     /locus_tag="VEW08_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HYJ40706.1"
                     /translation="MISNFALNSTEAAGSNASAPVDSVDAGDTGAFDALLALQSMVGE
                     DTVTEASAQMAGLLQGAELPGSLDDSEDSLADGDDASPEDALSFLAALLPLPQKPILS
                     EAAAAAPVSVPIQHEALPASAGGARPPGAGGAGTTDDLTQAMSFNLEGDGADSKPASM
                     NAAAAAPVIDSSTWSSMSGPAEMFAQSTRHAVERDSASLTTHVRDPRWADELGNRLAS
                     MVRTGESSASLQLSPVDLGPLEVNVAVRDNQATVHFGAANAETRALLEASIPRLREML
                     ASQGFQLMDSSVSHGFARQTRHESAGTPRVDAIGESPSATAALTHITGLLDLYA"
     gene            5525..6046
                     /locus_tag="VEW08_07950"
     CDS             5525..6046
                     /locus_tag="VEW08_07950"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HYJ40707.1"
                     /translation="MAQPAATDDELEDGVEAQPTPGRGKKILIIVVVLLLVLAGGGFA
                     AFKMMGGKEDPKKKEAVKKEQLLPARYVTLDPPFVVNFQAESTVRFLQITIGIMTRDV
                     EVEKLIKDNDPRIRNDLLLILGNQSYESVSKLEGKEDLRARSLEAVRAVVKDSGGEPN
                     KVEALYFTSFVMQ"
     gene            6056..7039
                     /gene="fliM"
                     /locus_tag="VEW08_07955"
     CDS             6056..7039
                     /gene="fliM"
                     /locus_tag="VEW08_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HYJ40708.1"
                     /translation="MAVEVLNQDEIDALLNGVDQGAVSTEPEAPPGEARQYDFSREMR
                     IVRGRMPTLEMVNERLARQLRVSIYNLLRRTVEIAVGQVSMKKFSEYTHSLSLPVNLN
                     LVKIHPLRGTGLFVIDPKLVFAMVDNFFGGVGRQAKIEGREFTATEMRVVHMFLRSAF
                     ADMKEAWQPIAKIDVEYVNSEINPNFAGIVTPSEVVVVCPFQIDLDGGGGSLHVVMPY
                     SMIEPLREVLDSGVQSDRAEQDDRWLTMLKDGLQDAEVELTTVLGSGSVALSKLVDLK
                     AGDVLPCDFNGKATVLAEGVPLFKGAYGVSRGQQCVRYEQKYRRNQNNSRN"
     gene            7044..7349
                     /gene="fliN"
                     /locus_tag="VEW08_07960"
     CDS             7044..7349
                     /gene="fliN"
                     /locus_tag="VEW08_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HYJ40709.1"
                     /translation="MNAKSEAVIADGDNQDVNLEVILDVPVTLSMEVGRTRIPIRNLL
                     QLNQGSVVELDRAAGEPLDVFVNGTLVAHGEVVVVNEKFGIRITDVVSPAERIRKLK"
     gene            7356..7748
                     /gene="fliO"
                     /locus_tag="VEW08_07965"
     CDS             7356..7748
                     /gene="fliO"
                     /locus_tag="VEW08_07965"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HYJ40710.1"
                     /translation="MDAPLFAAPAASAAPTAGGGLAEVTLALLAIVALIAGLAWLMKR
                     ARGFGTTGRDRIQVLGDRALGPKERCVLVRVGATEILIGVASGSVNTLHVFPEGTVTQ
                     ALAEPPAPPATTPSQPSFKALLMRSLGK"
     gene            7760..8518
                     /gene="fliP"
                     /locus_tag="VEW08_07970"
     CDS             7760..8518
                     /gene="fliP"
                     /locus_tag="VEW08_07970"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HYJ40711.1"
                     /translation="MKRVLSALGWLGLLLMPLLSSAAETTVTGLPAVTVTTAPGGGQT
                     YSLTLQVLILMTVLSLLPAILLMMTAFTRIVIVLAILRQAIGAGQTPPNQVLVGLALF
                     LTFFVMAPVIDKVNTEAAQPYLAGTIETTVALERAIGPLKGFMLEQTRETDIATFVRI
                     SGGKGFAKPADVPLSILVPAFVTSELKTAFQIGFLLFIPFVIIDLVVASVLMSMGMMM
                     LSPVLISLPFKIMLFVLIDGWALVMGTLAQSFFR"
BASE COUNT         1584 a         2785 c         2797 g         1446 t
ORIGIN      
        1 gcgcaggtgg tcgcgcaggt ggacatgacc accaccgaat ccacgcgcga acagttcaat
       61 ccgcagagcc aggtcgtgcg cagcgaatcg acggccgaag aagccgcacg caacggtgcc
      121 ggcaatgccg gtggcgtgcc cggcgcgctc accaaccagc ccccacagcc cggtgtcgcg
      181 ctgccgcctg gcgcgacgcc cgcggttgcc cagtccgggt caaattcggc cggtgcttcc
      241 gccgccggca cgaagcctaa caacgccgat gggacagtgg tcaccggacc ggattccacg
      301 tcgcggcagt tgacgcgtaa ctacgaaatc gaccgcacca tggcctacac acgtgccccc
      361 gccggccgcg tgacgcgcct gtcggtagcc gtgctcatcg acaatctgcg caccacggcc
      421 gcggacggca aggtcactga atcgccgctt cctccggagc agctcgaacg cctcaccgca
      481 ctggtgcgcg acgccgtggg ctttgacgcc acgcgtggtg acacggtcaa cgtcgtcaac
      541 tcttccttcc tcggcacgcc ggtgatcgag gagggcgaac tcgaatcgat tccattctgg
      601 gaacgtgcct gggcgcagac gctggcgaaa gtgctggcgg gacttctcgt gttgctggtc
      661 atcgtgttca gcgtgctgaa gccgctgacc aagggcctgc tgaccgccgc ccgtccgtcc
      721 gtgctgcgcc aggcggcgct cgcggccgcg aacgtcgggg gcggaggacc ggccgaagcg
      781 ccggctcttg cctatgaaca acaggttgcc gcggcgcgcg gcatggtcgc gcaggatccg
      841 aagcgcggcg cgcaggtcgt gaaggcatgg gtgcaaagcg atgcctgaag ccaccaaaac
      901 ccggaccggc gtggaacgcg ccgccattct gctgctgacg ctcggcgaga gcgaggcggc
      961 ggaaatcctc aagcacatgg gcgcgaagga cgtgcaacgc ctcgggcgtt ccatggccga
     1021 gctcaccagc gtgtcgcgtg acgaagtccg agatgtgctc aacaaattca cgagccagct
     1081 cgaagaccag acctcgctcg gcgtcggcag cgatgatttc gtgcgcaagg tgctggtcaa
     1141 cgcgctcggc gatgagaaag ccaactcgct gatggatcgc atccggctcg gcggccagcg
     1201 caagggcctc gaggcgctca agtggatgga ctcgcgtgca gtggccgagc tcattcgcaa
     1261 cgaacatccg cagatcatcg ccatcgtcct ttcatatctc gaagcggacc agggcgccga
     1321 agtactgggc ttcctgccgg agaacgcacg ctcggatctc atcgtgcgcg tcgcgacact
     1381 cgatggcgta cagccttcgg cgctcaccga gctcgacgag atgatggaga agcagttcgc
     1441 ggctggcgcc agcaatggca agagttcgtc gctcggcggc ctgaaggtcg cggccgagat
     1501 ggtcaatctg ctcgacgcca agatgggcag cgccgtcatg accgaagtcg gcaagaccga
     1561 cgagcggctc tcgcagagca tccaggacct gatgttcgta ttcgacgacc tgctcgagat
     1621 cgacgaccgt ggcatgcagg agctgctgcg gcaggtgccg gcggacaagc tgctgctgtc
     1681 gctcaagggc gccgacgaaa ctttcaaggc gaagatcttc aagaacatgt cgcagcgcgc
     1741 ggccgagatg atgaaggacg acctggccaa caagggcccg gtacgcctgg ccgacgtcga
     1801 agctgcacaa aaggaaatac tgctcgctgc ccgcaagctg gccgacgacg gcacaataaa
     1861 tctcggtggc aagggcggcg acgaatatgt ctgagcccct ggcgcgctgg gatctgccca
     1921 aggtcgaagg tgcttcgctg ccgcgtggca gcggtggcgt caacgtcatg cacctggccg
     1981 cggtcgagcg cgacgcgtgg gaacatggtt acaaggccgg ccacgtcgaa ggcgtacgca
     2041 agggcgaagc ggagctcaag gttcgcattg ccgaggtcga cgtcaaggtg acggcgttgc
     2101 aggcgatcat cgatacgctt gccaagcccc tggacgatct cgacaccggc atcgaaaccg
     2161 agctcacgcg gctggcgatg atcattgcga agcacctggt gagacgcgag ctcaggctcg
     2221 atcccgcgca ggtcattggc atcgtgcgac acacggtggg gctgttgccc ctcgccgcgc
     2281 gcaacatcaa ggttcatctg catcctgacg acgccgccat cctgcgcgat cggctcgccg
     2341 cgccggtggg cgaacgcgag tgggacctgc gcgaggatcc actcatggcg cgcggcggat
     2401 gccgcgtgac caccgacaat gcgagcatcg atgcccgagt cgagtcgacg atcgccgcgg
     2461 cgatgcgcgg cctgctcggc gacgatcgcg ccgagcgcgt cgaagcggcc gttgccgcgg
     2521 ccgacccgcc ggcggacgag gatccggcat gagcgcgacg aacgaggtcc gtaccgagcg
     2581 ttcgcgcacc tggcgcaaga gcatcgacat gcaggtggcg cgcgcggcga atctgccatc
     2641 gccgcccgtt gaaggttcat tgacgcgcat ggtcgggctc gcgctggaag cgtcgggctg
     2701 ccaggccgcg gtcggcgacg tctgcgatct catttccagt gacggctcgc gctgcgaggc
     2761 cgaagtggtc ggattctccg gcgacaagct gctcatgatg ccgacgggag acgtgcacgg
     2821 cctcgcgccg agcgcccgtg tgattccacg gcaacgcgcc ggcagcgtgc gcgtcggccc
     2881 gggattactg ggccgtatta ttgatggcac cggggcgccg ctcgacggcc tcggtccgct
     2941 gaatggcgac gaacgcgtga agctgcaggg caagagcatc aatccgctcg agcgccatcc
     3001 catcgattca ccgctcgacg tgggcgtgcg ttccatcaat tcattgctga ccgtcggacg
     3061 tggccaacgc atcggcctgt tcgcaggttc gggcgtcggc aagagtgttc tgctcggcat
     3121 gatggcgcgc tacacctcgg ccgacgtgat tgtcgtaggc ctgatcggtg aacgcggccg
     3181 cgaagtcaaa gagttcgtcg accgcattct cggacccgaa ggccgcaggc gttcggtggt
     3241 cgtcgcatcg ccagccgaca atccgcctct cattcgcctg catggcgcgt ggctggccac
     3301 gtccatcgcc gaatattttc gcgagcgcgg gttgtccgtg ctgctcatca tggattcact
     3361 gacgcggttc gcgcaggcac agcgcgagat cggtctcgcc atcggtgaag caccggccac
     3421 ccgcggctat ccaccctcgg tctttgcgcg cctgccggcg ctggtcgaac gcgccggcaa
     3481 cggctccacg ggccgcggtt cgatcaccgc cttttatacc gtgctcaccg aaggcgacga
     3541 ccagcaggat ccgatcgcgg acgcggcgcg cgccattctc gacggacacg tggtcttgtc
     3601 gcgacgcatc gccgaagcag ggcagtaccc ggccatcgac atcgaagcgt cggtcagccg
     3661 cgtgatgaac gaaatcgttt cgcccgagca cgccgagctc gcgcggcgtt tccgccagaa
     3721 cctggccgtc taccaacaac atcgtgatct catttccatc ggcgcgtacc agaagggcag
     3781 tgacccgcga gtcgacaccg ccatcgagct gtggccggcg atgcagaaat tcctcatgca
     3841 gccgatcgaa gagcgcgtcg gtcagcgtga ttccgtgcag gcgctggcac agttgtttgc
     3901 gtcggcgccc ccgccgccac gtaaggcggg cacatgacac gttccgaacg gctggacgtg
     3961 gtcaagcagg ccaccgcaca caacgaacgc gaacacgccg aacgcgtcgc tgcggccgag
     4021 cgtcacgtgg tggagatgga agagaagctc caggctctcg aacgttatcg caaggaatat
     4081 gaagacggat tcgccgcgcg cgccggagcc ggcctcggcg tggccgcgat gcgcgacttc
     4141 caggccttcc tggcgcgtct cggcgatgca ctcgaacagc aacgccaggt cgtcgagcag
     4201 gccagggctg cgcaccaggc caccatattg gtgtggcgcg aggccgccaa gcgggcgcac
     4261 gtggtcgata cgctggcaga gcgctggcag atcgaggagt cgcgcgagga aggccgccgc
     4321 gatcagcgcg acaacgatga gttgtcgcag caaatgcgtt tcaatcgaga gagaaaaaca
     4381 tgatttccaa tttcgcgctg aacagcaccg aagccgccgg cagcaatgct tcggccccgg
     4441 ttgattcagt ggatgccggc gataccggcg cattcgacgc cttgctggcg ctgcagtcga
     4501 tggtcggcga agacaccgtg accgaggcgt ctgcgcaaat ggccggcttg ctgcagggcg
     4561 cggagctgcc aggatcgctc gacgactccg aagattcact cgccgatggc gacgatgcgt
     4621 cgcccgaaga tgcattgtcg tttctcgccg cgctgttgcc cttgccgcag aagccaatac
     4681 tctccgaggc tgcggccgcg gcgcccgtat ccgtgccgat tcagcatgaa gcgttacccg
     4741 cgagtgcggg cggcgccagg ccgccgggcg ccggtggcgc aggcacaacc gacgatctca
     4801 cccaggccat gtctttcaac ctggagggtg atggcgccga ttcgaagccg gcttcgatga
     4861 atgccgccgc cgcggcaccc gtcatcgatt cgtctacgtg gagctccatg agtggcccgg
     4921 cggaaatgtt cgcccagtcc acgcgtcacg ccgtggagcg cgactcggcc tccttgacca
     4981 cccatgtccg cgatccgcgc tgggccgatg agctcggcaa ccggctcgcg tcgatggtgc
     5041 gcaccggcga atcgtcggcg tcgctgcaat tgtcaccggt cgatctcggg ccgctggaag
     5101 tcaatgttgc cgtgcgcgac aaccaggcga ccgtgcactt cggcgccgcg aacgccgaga
     5161 cgcgcgcttt gctggaagcg tcgattccgc gattgcggga aatgctcgcg tcgcaggggt
     5221 ttcagctgat ggacagcagc gtctcgcatg gcttcgcgcg ccagacccgt cacgaatccg
     5281 ccggcacgcc gagagtcgat gcgatcgggg aatccccgag cgcgactgcg gcgcttacac
     5341 acatcacggg tctgctagac ctgtacgcct gattcgccgc aggcctgatc ggcagccgct
     5401 ttcgcggcac gccaaaaaac cgtcaccgat gacagccatt gcgtgacgtc gctcacgctt
     5461 tggacgtggc acgcgcgttg caccagtcaa tgccgtcgca accacttttc cgcgtgagga
     5521 ttccatggcc cagccagccg caaccgacga tgaactcgaa gatggcgttg aagcccaacc
     5581 gacccccggt cgtggcaaga agatcctgat catcgtcgtc gtgctgctcc tggtgctcgc
     5641 cggcggcggt tttgccgcgt tcaagatgat gggcggcaag gaagacccga agaagaagga
     5701 agcggtcaag aaggagcaat tgctgccggc gcgctacgtc acgctggatc cgcccttcgt
     5761 cgtgaatttc caggccgaat ccaccgtgcg attcctgcag atcaccatcg gcatcatgac
     5821 gcgcgatgtc gaagtcgaaa aactgatcaa ggacaacgat ccgcgcatcc gcaatgacct
     5881 gctcctgatc ctcggtaacc agagttacga aagcgtctcc aagctcgagg gcaaggaaga
     5941 tctgcgcgca cgcagcctcg aagccgtacg cgcggtggtc aaggattcgg gcggcgaacc
     6001 caacaaggtc gaagccctct acttcacttc gttcgtgatg cagtagggcg ccgacatggc
     6061 cgttgaagtt ctcaaccagg atgaaatcga cgcgctgctc aatggcgtcg accagggcgc
     6121 cgtcagcacc gaacccgaag cgcctccggg cgaagcgcgc cagtacgact tcagtcgtga
     6181 aatgcgcatc gtgcgcggcc gcatgccgac gctcgagatg gtcaatgagc ggctcgcccg
     6241 ccagctgcgc gtgagcattt acaacctgct gcgacgcacg gtcgaaatcg cggtggggca
     6301 ggtgtccatg aagaagttca gcgagtacac gcactcgttg agcctgccgg tcaacctgaa
     6361 cctcgtgaag atccatccgc tgcggggcac gggcctgttc gtcatcgatc cgaagctcgt
     6421 gttcgcgatg gtcgacaact tcttcggtgg cgtcggccgc caggcgaaga tcgagggccg
     6481 cgaattcacc gccaccgaaa tgcgcgtggt gcacatgttc ctgcgcagcg cgttcgcgga
     6541 catgaaagaa gcctggcagc ccatcgccaa gatcgacgtc gagtacgtga actcggagat
     6601 caaccccaat ttcgccggca tcgtcacccc ttcggaagtc gtggtggtgt gcccgttcca
     6661 gatcgacctc gatggtggcg gcggctcgct gcacgtcgtc atgccgtact cgatgatcga
     6721 gccgctgcga gaagtgctcg attcgggcgt gcagagcgat cgcgctgaac aggacgatcg
     6781 ctggctcacg atgctcaagg acggcctgca ggacgcggag gtggaactga cgacggtgct
     6841 cggaagcggc agcgtcgcct tgtcgaagct cgtcgatctc aaggccggtg acgtactgcc
     6901 ctgcgacttc aacggcaaag ccacggtgct cgccgagggc gtgccgctgt tcaagggtgc
     6961 gtacggcgtc tcccgcggcc agcagtgcgt ccggtacgaa cagaaatacc gccgcaatca
     7021 aaacaactcg aggaattgaa accatgaatg cgaagagtga ggcggtgatc gccgacggcg
     7081 acaaccagga tgtgaacctc gaggtgatcc tcgatgtccc cgtcaccctg tccatggaag
     7141 tggggcgcac acgcatcccg atccgcaacc tgctgcagct caaccagggc tcggtggtgg
     7201 aactggatcg cgcggccggc gagccgctcg acgtgttcgt caacggcacg ctggtggcgc
     7261 acggcgaagt ggtggtggtc aacgagaagt ttggcatccg catcaccgac gtggtcagcc
     7321 ccgccgaacg catccgcaag ctcaagtaag cgcgcgtgga cgcccctttg tttgccgcgc
     7381 ccgccgcgag cgccgccccg actgcgggtg gtggcctcgc cgaagtgacg ctggccctgc
     7441 tggcaatcgt cgcgctcatc gcaggcctcg cctggctgat gaagcgagcg cggggttttg
     7501 gcacgaccgg acgcgaccgc atccaggtcc tgggtgaccg tgcgctgggc ccgaaagagc
     7561 gctgcgtgct cgtgcgcgtc ggcgccaccg aaattctgat cggcgtcgcc tcgggcagcg
     7621 tgaacacgtt gcacgtcttt cccgaaggca ctgtgactca agcgttggcc gaaccgcccg
     7681 cgccaccggc caccacgccc tcgcagcctt cgttcaaggc cctgctgatg cggagccttg
     7741 gtaaatgaag agtgggcaaa tgaaaagagt tctctccgca ttgggctggt taggcctgtt
     7801 gctgatgccg ctgctgagct ccgccgccga aacgacggtg accggccttc cggccgtcac
     7861 cgtgaccacc gcgccgggcg gcgggcagac ctactcgctg acgctccagg tcctgatcct
     7921 gatgacggtg ctgtcgctgc tgcccgccat cctgctgatg atgacggcgt tcacgcgcat
     7981 cgtcatcgtg ctggcgatac ttcgccaggc gatcggcgcc ggccagactc cgcccaacca
     8041 ggtgctcgtg ggcctggcgc tgttcctgac tttcttcgtg atggccccgg tgatcgacaa
     8101 ggtgaacacc gaagccgcgc agccctatct cgccggcacc atcgagacca cggtcgcgct
     8161 cgaacgcgcc atcggcccgc tcaagggctt catgctcgaa cagacccgcg aaaccgacat
     8221 cgcgacgttc gtgcgcattt ccggcggcaa gggtttcgcg aagccggccg acgtgccgct
     8281 cagcatcctg gtgccggcgt tcgtcaccag cgagctcaag accgcgttcc agatcggctt
     8341 cctgctgttc atcccgttcg tgatcatcga tctcgtggtc gcgagcgtac tcatgtccat
     8401 gggcatgatg atgttgtcgc cggtgctgat ctcgctgccc ttcaagatca tgttgttcgt
     8461 gctcatcgac ggatgggcgc tggtcatggg cactctggcg caaagttttt tcaggtaggt
     8521 agcacacaat gactcccgaa atggtggtga cagtcggccg gcacgcgctc gagatgacgc
     8581 tcatgctctc ggcgccgctg ctgctcacgg cg
//
LOCUS       DASQJS010000194         4396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_734722, whole genome shotgun sequence.
ACCESSION   DASQJS010000194 DASQJS010000000
VERSION     DASQJS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734722"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..376
                     /gene="ihfA"
                     /locus_tag="VEW08_07975"
     CDS             77..376
                     /gene="ihfA"
                     /locus_tag="VEW08_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HYJ40712.1"
                     /translation="MALTKAEMAEALFNQLGLNKREARELVDLFFEEVRHALSNGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEEIPISARRVVTFRPGQKLKARVEAYAGTKE"
     gene            357..713
                     /locus_tag="VEW08_07980"
     CDS             357..713
                     /locus_tag="VEW08_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYJ40713.1"
                     /translation="MLEPKNNDELPAIPGKRYFTIGEVSELCGVKPHVLRYWEQEFPQ
                     LKPVKRRGNRRYYQRQDVIIIRQIRSLLYEQGFTIGGARNKLGGEEARVDVSQSQQIV
                     KQMRVELEELLRMLRR"
     gene            complement(739..1665)
                     /locus_tag="VEW08_07985"
     CDS             complement(739..1665)
                     /locus_tag="VEW08_07985"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HYJ40714.1"
                     /translation="MRATVLIIITCLATLTACARKPQNFETPEAAVQALIEAAGADSE
                     RPLLAVLGKDAKPLVDSGDPVADKNAREGFLEAYKAANSLDKTDPEFITLEVGEDKWP
                     FPIPIVSEGGKWHFDSPTGVDELINRRVGANELDTIQSCLAYADAQREYYMRNPENSQ
                     LLHYAGRLVSTEGKKDGLYWPAGENEEQSPLGEGFAQARAEGYLQQSTSMKGVPFHGY
                     IYRLLTKQGANAPGGAYDYLVNDQLLGGFAAVAFPAEYGNSGVMTFIVNHDGVVYSKD
                     LGPETAKLALAIDTFDPGSDWKKEVGEQTAAN"
     gene            complement(1683..3053)
                     /locus_tag="VEW08_07990"
     CDS             complement(1683..3053)
                     /locus_tag="VEW08_07990"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HYJ40715.1"
                     /translation="MKRALAICGTVAAAAIIAGSAVVSAQTTPPAQPAATDAPAASKT
                     PPFKKEELEALVAPIALYPDALLAQIFMASTYPLEIVEAARWSKANPSVTGDAVAGAV
                     QKETWDPSVKSLCAFPTVLAQMNEKIDWTQKMGDAFLAQQKEVMQAVQILRGKAKDTG
                     NLKSTKEVTVKEEPAPAGSPAPQTIIIESPDPEVVQVPVYNTSVVYGTWGYPYYPPYS
                     YYPPGYAYGAAFWSFTAGVIVGGAIWGNCNWGGNDVNIDIDRQNNFNRNEINNNRNSN
                     NRGNSATQNNRGGNKQSWNHNAEHRKGVGYKDSATQQKYGKGASQRDTQAREQYRGRA
                     EQGRQQISREGVGNQRDLAGNSVGTRDMGGASTRDLGGGTRDLGGNNAGTRDLGGAST
                     RDLGGSSTRDLGGSSNRSSNRSSSSSAFGGSNRGSGASTRSSSSRGGMSRGGGGMSRG
                     GGGRRR"
     gene            3384..>4396
                     /locus_tag="VEW08_07995"
     CDS             3384..>4396
                     /locus_tag="VEW08_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HYJ40716.1"
                     /translation="MTGTAVTFGTFDWAIVALYFVANTAICIWCAMQKEKNTADYFLA
                     SRSAGWFLIGSSIFASNIGAEHLIGLAGSGADSGMAFAHWELHSYLVLVLGWIFAPVY
                     LRAKVFTTPEFLERRYTPGTRTCLSIIFLFAYVLTKASVTIFAGAFAIQTILGYQTID
                     LPLFGATDFFWFSAFSLVIITGVFVVLGGMKSVLWTEAMHVPVLLVGSAVLLFVGLGQ
                     IGGLDAMVAVNEAVNPDSVHLWRDLSTTPETQGFPGFLFDPSTTPWLGVLLCSPIIGL
                     WYWCTDQYIVQRVLTARGLKEARRGTIFAAYLKLAPVFIFLFPGMIAVALWKQGATGF
                     ENIG"
BASE COUNT          723 a         1365 c         1360 g          948 t
ORIGIN      
        1 gacgcggacc gtttgttagc cgcgattcgt gccgatctga gtgcaacgtt gggcgcagga
       61 tttcgagaat aaaagcatgg ctctgacgaa agcggagatg gctgaggcgc tgttcaatca
      121 gctgggtctc aacaaacgcg aggcgcgcga gctggtggac ctgtttttcg aggaagtgcg
      181 tcatgcgctg tccaacgggg aacaggtcaa gctctcgggc ttcgggaact tcgatttacg
      241 cgacaagaac cagcggccag gacgtaatcc gaaaacggga gaggagattc ccatcagcgc
      301 gcgacgcgtt gtgacgttcc gtccgggtca gaaattgaag gcgcgagtcg aagcttatgc
      361 tggaaccaaa gaataacgac gagctgccgg cgatcccagg caagcgctat ttcacgatcg
      421 gtgaggtgag cgagctctgc ggcgtgaagc cgcacgtgct gcgttattgg gaacaggaat
      481 ttccgcaact caagccggtc aagcgccgcg gcaaccgccg ctactaccag cgccaggacg
      541 tcatcatcat ccgccagatc cgcagcctgc tctatgagca gggcttcacg attggtggtg
      601 cccgcaacaa gctgggcggt gaagaagccc gcgtcgacgt cagccagagt cagcagatcg
      661 tcaagcagat gcgcgtggag ctcgaagagc tgctgcgcat gctccggcgc tgaatttcgc
      721 cggcgcccgc agcgaacctc agttcgccgc ggtttgttcg cctacttcct tcttccagtc
      781 cgagcccggg tcgaacgtat cgatggccag ggcgagcttc gccgtctcgg ggccaaggtc
      841 cttcgaatag accacgccat cgtggttcac gatgaaggtc atgacgccgg aattcccata
      901 ctcagcggga aaggcgacgg ctgcgaatcc acctagcagt tggtcgttga cgagatagtc
      961 ataggcgccg cccggcgcgt tcgctccctg cttggtcagc aggcggtaga tgtagccgtg
     1021 gaagggcacg cctttcatcg acgtgctttg ctgcaagtac ccctccgcgc gtgcctgtgc
     1081 gaacccttca ccgagcgggc tctgttcttc gttctcgccc gcgggccagt acaggccgtc
     1141 cttcttgcct tcggtgctga cgagtcgtcc cgcgtaatgc agcaactgcg aattctccgg
     1201 attacgcatg tagtactcac gctgcgcgtc ggcgtaggcg aggcatgact ggatggtgtc
     1261 gagctcgttg gcacccacac ggcggttgat gagctcgtcg acacccgtcg ggctgtcgaa
     1321 atgccacttt ccgccttccg agacgatcgg gatcgggaag ggccatttgt cttcgcccac
     1381 ttcgagcgtg atgaattccg gatcagtttt gtcgagcgag ttcgcggcct tgtaagcctc
     1441 gagaaagccc tcgcgcgcat tcttgtcggc aaccgggtcg cccgagtcga ccagtggttt
     1501 cgcgtcttta ccgagcaccg cgagcagggg gcgctcgctg tccgcgccgg cggcctcgat
     1561 gagggcctgc accgcggctt cgggcgtttc aaaattctgc ggcttccttg cgcacgccgt
     1621 cagtgtcgcg aggcacgtga tgatgatcag tactgttgcg cgcattgcgt tatttccctg
     1681 tttcatcgcc gccgtcctcc gccgccacgt gacattccgc cgccgccgcg cgacatgccg
     1741 ccccggctgc tggatgaacg cgtgcttgcg ccgctgccgc gattggagcc gccaaaagcg
     1801 ctggatgatg aagatcgatt gctcgaccgg tttgaactgc cacccaagtc gcgagtggat
     1861 gagccaccga gatcgcgggt tgacgcaccc cccaggtctc gtgtgcccgc gttgttgcca
     1921 cccaggtcgc gcgtgccgcc accgagatca cgcgtggaag cgccacccat atcccgcgtg
     1981 ccgacgctgt tgcccgccag gtcgcgctgg ttgccgacgc cttcacgcga tatctgctgc
     2041 cgaccctgtt ccgcgcggcc gcgatattgc tcgcgcgcct gcgtgtctct ctggctcgcg
     2101 cccttgccgt atttctgctg cgtggccgaa tccttgtagc cgacgccctt gcgatgttcg
     2161 gcgttgtggt tccaggactg cttgttgcca ccgcgattgt tctgggttgc gctgttgccg
     2221 cggttgttgc tgttgcggtt gttgttgatc tcgttccggt tgaaattgtt ctgccggtcg
     2281 atatcgatat tgacgtcgtt gccgccccag ttgcagttac cccagatcgc gccgccgacg
     2341 atgactcccg cagtgaatga ccagaacgcc gcgccgtacg catagcctgg cgggtaatag
     2401 gagtacggcg ggtagtacgg gtagccccag gtgccgtaca ccacgctggt gttgtacacc
     2461 ggcacctgga caacttccgg atccggcgac tcgatgatga tggtctgcgg tgcgggtgag
     2521 ccggccgggg ccggctcttc cttgaccgtc acttccttgg tgctcttgag attgccggtg
     2581 tccttggcct tgccgcgaag gatctgcacg gcctgcatga cttctttctg ctgggccagg
     2641 aaggcgtcgc ccatcttctg cgtccagtcg atcttctcgt tcatctgtgc gagcacagtc
     2701 gggaaggcgc acagcgattt cacgctgggg tcccaggtct ccttttgcac cgctccggcg
     2761 acggcatcac ccgtgaccga agggttggcc ttggaccagc gcgcggcttc cacgatctcc
     2821 aatggatagg tggacgccat gaaaatctgc gcgagcaggg catctggata tagcgcgatg
     2881 ggcgcaacga gtgcctcgag ctcttctttc ttgaagggag gtgttttgga cgcagccggt
     2941 gcgtccgtcg cggctggctg tgcaggtggg gtggtctggg cgctgaccac tgccgatccg
     3001 gcaatgattg cagcggctgc tacggttccg cagatcgcaa gcgcgcgctt catttttgat
     3061 atccctcgcg ctcattccgt ggccttagag agcgcttctc atccaatact cgcgcaggcg
     3121 cgacgtgcgc cgaaatggta cgaagtgcct acaccgagcc attgcaatcc gctggaagct
     3181 tccggcatcg cgctgccccg gaacgccgaa tcaacgatct gcgcccctgt gcactcgcat
     3241 tgattgcggc gcttcaccat cggcttttcg cacactaagc tcctcgccca cgtggcacct
     3301 ggggagtggc cacggcccaa agttttatcg cgcctgccgg cgaaatccgg cgggacctac
     3361 cggttgttgc atgaggggag atcatgacgg gtaccgcagt cacattcggg acgttcgact
     3421 gggccatcgt tgccctgtac ttcgtcgcca acacggccat ctgcatctgg tgcgcgatgc
     3481 agaaagaaaa gaatacggcc gactacttcc tggccagccg cagcgccggc tggttcctga
     3541 tcggctcctc gatcttcgcc tcgaacatcg gcgcggaaca cctgatcggt ctcgccggct
     3601 ccggcgcgga ctccggcatg gcgttcgcgc actgggagct gcattcctat ctggtgctgg
     3661 tgctcggatg gatcttcgcg cctgtctatc tgcgcgcgaa ggtgttcacc acgccggaat
     3721 ttctcgagcg acgctacacg ccaggcaccc gcacctgtct ttcgatcatc ttcctgttcg
     3781 cgtacgtact caccaaggca tcggtgacca tctttgccgg agcgttcgcg atccagacca
     3841 tcctcggcta tcagacgatc gatctgccgt tgttcggcgc cacggatttc ttctggttct
     3901 ccgccttctc gctcgtgatc atcaccggcg tgttcgtcgt gctcggcggc atgaagtccg
     3961 tgctgtggac cgaagcgatg cacgtgcccg tgctgctcgt gggttccgcc gtgctgctgt
     4021 tcgtaggttt ggggcaaatc ggcggactgg atgccatggt cgccgtcaat gaggcagtga
     4081 atccggacag cgtccacctg tggcgcgacc tctcgacgac gccggagacg cagggtttcc
     4141 cgggcttcct gttcgatccc agcaccacgc cgtggctcgg cgtgttgctg tgctcgccca
     4201 tcatcggcct ctggtactgg tgcacggacc agtacatcgt gcagcgcgtg ctcaccgcgc
     4261 gcgggctcaa ggaagcgcgc cgcgggacga tattcgccgc ctatctcaag ctcgctccgg
     4321 tattcatctt cctgtttccc ggaatgatcg cggtggcact gtggaagcag ggtgcgacgg
     4381 gctttgagaa catcgg
//
LOCUS       DASQJS010000195         9179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4504487, whole genome shotgun sequence.
ACCESSION   DASQJS010000195 DASQJS010000000
VERSION     DASQJS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9179
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4504487"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /gene="trxB"
                     /locus_tag="VEW08_08000"
     CDS             complement(<1..909)
                     /gene="trxB"
                     /locus_tag="VEW08_08000"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020796668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HYJ40717.1"
                     /translation="MSTSRHSPLVILGSGPAGYTAAVYAARANRKPLLITGVEQGGQL
                     MTTTEVDNWPGDAHGLLGPALMERMKAHAERFNTEIVSDHIHTVDFSKRPFVLNGDYG
                     SYTCDALVIATGASAKYLGIPSEDKFRGKGVSACATCDGFFYKGHRVMVVGGGNTAVE
                     EALYLSNLASHVTVVHRRDKFRAEKILQDRLFALEKTGKVRIAWNHTVDEIVGDQTGV
                     NGVHIKPTEHGGTIETLDVTGAFIAIGHTPNTGIFAGQLDMRDGYITIKSGSEGAATA
                     TSVPGVFAAGDVADHVYRQAITSAGSG"
     gene            1040..2098
                     /gene="ald"
                     /locus_tag="VEW08_08005"
     CDS             1040..2098
                     /gene="ald"
                     /locus_tag="VEW08_08005"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HYJ40718.1"
                     /translation="MRIGIPRELKPREGRVGLIPAACVELVRGGHEVFVEHGAGEKSG
                     YTDQHFRDVGVTVVPGADELFDKSQMIVKVKEPIEPDLKRLRKDHLLFCYLHLAALPE
                     LTRRLVDIGLTAVAFETVEDRTGRLPLLAPMSDIAGRLSMQYATTLLHQPQGGKGILL
                     GGVPAAKRGRVVVLGGGVAGGNSALVAAGLGAEVIVFDKDRDKLVAARAMGANVTGLY
                     PHVADVADAVREADVVIGAVLVTGARAPHVVSAAMVRTMEAGSVIVDISVDQGGCIET
                     TRPTSWEAPTYQWEGVTHFCVTNMPGSVPRTSSQALSAAIAPFAVRLADGDWREDAGI
                     VRGINVEGGKVTHPALLS"
     gene            2137..4464
                     /locus_tag="VEW08_08010"
     CDS             2137..4464
                     /locus_tag="VEW08_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HYJ40719.1"
                     /translation="MAETTYDRTKPQKRFTAAVSRGLRESAVLAIGVVALVMLVALLS
                     YQREDSAFSSTGGGDHAVRNLIGPFGAWLSDILYFLFGRPAYLFPVMLGVACFVMFRR
                     DDEEGRTRANTAVRIGGFTLMLFASCGLATLHWSAGTLRESAGGVVGQFVGTGLAGNL
                     NFLGATLVLLACWMAGVSLAFGVSWLTIVDKIGAGIWNAIGWVKARRSTARDVAVGLE
                     AKKARVEAAKVEEKRVATRVKPRIEAPAAVIEKSERVEKERQVPMFDAPKSGALPPLT
                     LLDDPPTHEAHYSSEALEAMSRLVELKLKDFGVEVEVVAVQPGPVVTRFEMRPAPGVK
                     VSQITGLAKDLARSLSAISVRVVEVIPGKSVMGLEIPNEKREIVTLGEIIKSKAYDDV
                     ASPLAIALGKDIGGSPVCADLAKMPHLLIAGTTGSGKSVGINAMILSLLYKSTAEHVR
                     LIMIDPKMLELSVYEGIPHLLAPVVTDMKHAANALRWCVAEMERRYALMAALGVRNIS
                     GFNRKVKDATEAGKPIRDPVMTAAAANDPTIDQRLIQDLVPLPYIVVVIDELADMMMI
                     VGKKVEELIARLAQKARASGVHLILATQRPSVDVITGLIKANIPCRIAFQVSARVDSR
                     TILDQMGAESLLGHGDMLYLQSGTSVPTRVHGAFVSDAEVHRVVESLKRSGAPNYIED
                     VLEGPSVPLAGISGEPQAEGGDAGGEQDALYDEAVKIVTTERKPSISYVQRRLKIGYN
                     RAARLLESMENAGVVGPLQPNGAREILVPAPPGSE"
     gene            4461..5117
                     /gene="lolA"
                     /locus_tag="VEW08_08015"
     CDS             4461..5117
                     /gene="lolA"
                     /locus_tag="VEW08_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HYJ40720.1"
                     /translation="MSKRTMRVAGLLASLGMTGAVQAQTPLDSYLEHLKTLRAEFSQV
                     VTDGKGREVQNARGKLTIVRPGRFRWELTPSVGTSSSPQLMVADGKNLWFYDRDLEQV
                     SVKPASSALTATPAGLLSGEGNIRELFTVAPAGRKDGLDWVLVTPKENDADFREARLA
                     FGPGPRKEGELKRMVLKDKLGQTVRLDFDTSERNPPVAEAEVKFIPPADADVIGTPAK
                     "
     gene            5114..5500
                     /locus_tag="VEW08_08020"
     CDS             5114..5500
                     /locus_tag="VEW08_08020"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HYJ40721.1"
                     /translation="MNLQRVWWTLGVVFVVLAMVVCLVPAPQVPSPMEWNDKVSHLAG
                     HGLLAVYFAGLVPRRSWWKIFLFLLVFGAVIEFAQYYTHLGREGDPRDVLANSAGAVL
                     GLVAARLGLARWPDLAAWLLGRRATQ"
     gene            5497..6861
                     /locus_tag="VEW08_08025"
     CDS             5497..6861
                     /locus_tag="VEW08_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016486902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HYJ40722.1"
                     /translation="MSSRGAGNPGGQPDFLRDVPSDDALRPLADRMRPRTLDEFVGQE
                     QVLGSGKPLRQALEKGQLHSLILWGPPGTGKTTLARLIARRADAEFMQLSAVMAGVKD
                     IRESVERARTARAERGRRSILFLDEVHRFNKAQQDTFLPYVEDGTLTFIGATTENPSF
                     EVISALLSRARVYVLRSLGVDDIVKLLRRALEDSGRGLGQRNIDIDDEALVALAHGGD
                     GDARRALGMLEVAADLATEHEGRARITIEQVREVATGSRRRFDKGGEQFYDQISALHK
                     AVRGTDPDAALYWFARMLDGGCDPHYLARRIVRMAVEDIGLADPRGQQLALDAWQTFE
                     RLGSPEGELALANALVYLAVAPKSNAVYVAYGEARADVEQFGTLDVPLRFRNAPTKLM
                     KGLGYGEGYQYAHDAPDAFVKGERYLPDEMPDRRYYRPAPRGLEIKIGEALARLRKPE
                     EDKS"
     gene            6872..7237
                     /locus_tag="VEW08_08030"
     CDS             6872..7237
                     /locus_tag="VEW08_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HYJ40723.1"
                     /translation="MNWLWVGIGGALGSVARYGAYRLWPSSPGGFPVPTFTVNLLGSF
                     AIGLIYMYVAARGASAENARLFWMTGVLGGFTTYSAFALETALLGFSWTGVAYVGATV
                     FGCLAAAFAGLKMGTFLVS"
     gene            7250..8530
                     /gene="serS"
                     /locus_tag="VEW08_08035"
     CDS             7250..8530
                     /gene="serS"
                     /locus_tag="VEW08_08035"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HYJ40724.1"
                     /translation="MLDAKQLRADPAAVAANLARRGFTLDVGAFTILDELRKQAQIES
                     DRVRAERNANAKSVGMAKGKGEDASALLARAEELTNKLASSDAAIASVQQQLDAWQLG
                     LPNTLHASVPDGRDESANVEIRRIGEPRKFDFEPKDHVELGEKLGGLDFETAAKISGA
                     RFSVMRGGLARLHRALAQFMLDLHTRDHGYTELYVPYLVNASTLQGTGQLPKFEADLF
                     RVQGEQGFYLIPTAEVPATNLVRDSIVDPKQLPMRFVAHTPCFRSEAGAAGKDTRGLI
                     RQHQFEKVELVHIVKPADSYDALEELTSHAEQVLQRLELPYRVIALCAGDVGFSAAKT
                     YDIEVWLPAQNKYREISSCSNCEGFQARRMQARWRNPDTGKPEPLHTLNGSGVAVGRA
                     LVAVLENFQNADGSITVPDALRPYMDGMGTIPIS"
     gene            8549..>9179
                     /locus_tag="VEW08_08040"
     CDS             8549..>9179
                     /locus_tag="VEW08_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40725.1"
                     /translation="MNVGVPKALSLALRMQELNRWLSAQEGSAGETHEFFAMPHGNAL
                     VTIDPTSEAPAASYNRNRIALCGARGGLTQDGLRELLDMFASRRIPRVFAWLSPGPDD
                     ALVRDWLHELSFTRVPWTRYPTMMFSAIADEFRRTQFEIRQVEMAEFPALRDELDDVL
                     MEGYEHTLGKPGFHHYLLFDEGRPIALAALVKFGEIGYLTYAGTIERERR"
BASE COUNT         1608 a         2887 c         3040 g         1644 t
ORIGIN      
        1 tccggatccg gccgaggtga tggcctgacg atacacgtga tcggcgacgt cgccggcggc
       61 gaacactccg ggcacgctgg tggcagttgc ggcgccttca ctgccgctct tgatggtgat
      121 gtagccgtcg cgcatgtcga gttgtccggc gaagatgccg gtgttcggcg tgtgcccaat
      181 ggcgatgaat gcgccggtga cgtccagggt ttcgatggtg ccaccatgtt ccgtcggctt
      241 gatgtgcacg ccatttacgc cggtctggtc accgacgatt tcatcgacgg tgtgattcca
      301 ggcgatgcgc accttgccgg tcttctcgag agcgaacagg cgatcctgca ggattttttc
      361 cgcgcggaat ttgtcacggc ggtgcaccac ggtcacgtgg ctggcgaggt tggatagata
      421 gagcgcttct tcgaccgcgg tgttcccgcc gcccacgacc atgacgcggt ggcccttgta
      481 aaagaagccg tcgcaggtcg cacaggccga aacgcccttg ccgcggaact tatcttccga
      541 cgggatgccc agatacttgg cggaggcgcc ggtggcgatg accagtgcat cgcaggtgta
      601 actgccgtag tcgccgttca gcacgaacgg acgcttcgag aagtccaccg tatggatgtg
      661 atcgctgacg atttccgtgt tgaaacgctc ggcgtgcgcc ttcatgcgtt ccatcagcgc
      721 ggggcccagc aggccatggg cgtccccggg ccagttgtcc acttccgtgg tggtcatgag
      781 ttggccgcct tgctccacac cggtgatgag cagcggtttg cgattggcgc gcgccgcata
      841 aacggcagcg gtatagccgg cggggccgga accgaggatg acgagaggac tgtgtcgaga
      901 agtgctcatg tataatttcg gccattcaaa tggcgttcaa gcctcataga ttgcgcgcag
      961 gcacgctgtt ttcaatcttc ctttcgggtc aggcgggcgc gccgccttat cgaccgagcg
     1021 acatgttaac gggacaccca tgcgtattgg cattcctcgt gagttgaaac cccgcgaggg
     1081 gcgagtcgga cttattcccg cggcctgcgt cgagctggtg cgcggcggac acgaagtgtt
     1141 cgtcgagcac ggcgccggcg agaagagtgg ctatacagat caacacttcc gcgacgtggg
     1201 cgtcacggtg gtgccgggcg ccgacgagct cttcgacaaa tcccagatga tcgtcaaggt
     1261 caaggagccc atcgagcctg acctgaaacg tctgcgaaag gaccacctgc ttttttgtta
     1321 tttgcatctc gcggcgttgc cggagctcac gcggcgcctg gtcgacatcg ggctcaccgc
     1381 ggttgcgttc gagacggtgg aagaccgtac cggccgcctg ccgctgctcg ctcccatgag
     1441 cgacatcgcc ggccggcttt cgatgcagta tgcgaccacc cttctgcacc agccccaggg
     1501 cggcaagggc atcttgttag gtggcgtacc cgcggcgaaa cgcggccggg tcgtcgtgct
     1561 cggcggcggt gtggccggcg gcaattcggc attggtggcc gcgggcctcg gtgccgaggt
     1621 catcgtgttc gacaaggatc gcgacaagct ggtcgccgcg cgcgcgatgg gcgccaatgt
     1681 cacgggcttg tatccgcatg tcgctgacgt ggccgacgcg gtgcgcgagg cagacgtggt
     1741 catcggcgcg gtgctggtca cgggcgcgcg ggcgccgcac gtcgtgtccg ccgccatggt
     1801 gcgcaccatg gaggccggca gcgtcatcgt ggacatctcc gtcgaccagg gcgggtgcat
     1861 cgaaacgacg cggcccacgt cctgggaagc acccacgtat cagtgggaag gcgtcacgca
     1921 tttctgcgtc accaacatgc ccggctcggt gccgcgcacc tcctcgcagg cgctgtcggc
     1981 cgcgatcgca ccatttgccg tccggttggc cgacggcgac tggcgcgagg acgcgggtat
     2041 tgtccggggc atcaacgtcg agggcggcaa agtgacccac ccggcactgc tgagctgata
     2101 tctttaccgc agtccgaaca acgacatagc gagcaaatgg cagaaaccac ctacgatcgt
     2161 accaagccgc agaagcgctt caccgcagcg gtgtcgcgtg ggctgcggga gagcgccgtc
     2221 ctggccatag gcgtggtcgc gctggtcatg ctcgtggcgc tgctctcgta ccagcgtgaa
     2281 gactccgcgt tctcttcgac cggcggtggc gatcacgcag ttcgcaatct gatcgggccg
     2341 ttcggcgcct ggctttcgga cattctgtat ttcctgttcg gccggcccgc gtacctgttc
     2401 cccgtcatgt tgggcgtagc ctgcttcgtg atgttccggc gggacgacga ggaaggccgc
     2461 acgcgcgcga atacggccgt tcgcatcggc ggattcacac tcatgctttt cgcgagctgc
     2521 gggctggcca ccctgcactg gagcgccggt acgttgcgcg agagcgcggg cggagtggtg
     2581 ggccaatttg tcggcacggg tctcgcgggc aatctgaatt ttctcggcgc caccctggtt
     2641 ctgctggcct gctggatggc gggtgtttcc ctggctttcg gagtctcctg gctcaccatc
     2701 gtcgacaaga tcggcgccgg catctggaat gccatcggct gggtcaaggc ccggcgcagc
     2761 accgcccgtg acgttgccgt cggcctcgaa gccaagaagg cgcgggtcga agcggccaag
     2821 gtggaagaga agcgcgtggc cacgcgcgtc aagccgcgca tcgaagcgcc cgcggcggtg
     2881 atcgaaaaga gcgagcgtgt cgagaaggag cgccaggttc cgatgttcga cgcgccgaag
     2941 tccggcgcat tgccgccgct cacattgtta gacgacccgc cgacacacga agcgcactat
     3001 tcctcggaag cgctcgaggc catgtcgcgg ctggtcgaac tcaagctcaa ggacttcggc
     3061 gtcgaagtgg aagtggtggc cgtgcagccc ggcccggtgg tgacccgatt cgagatgcgc
     3121 cccgctccgg gtgtcaaggt gagccagatc accggtctcg ccaaggacct cgcgcgttcg
     3181 ctctcggcca tcagcgtacg cgtggtggag gtcatccccg gcaagtcggt catgggcctg
     3241 gaaatcccga acgagaaacg cgagatcgtc acgctcggcg aaatcatcaa gtcgaaagcc
     3301 tacgacgacg tggcatcgcc gctcgccatc gcgctcggca aggacatcgg cggctcgccg
     3361 gtgtgcgcgg atctcgcgaa gatgccgcac ctgctcatcg cgggcaccac cggctccggt
     3421 aaatcggtcg gcatcaacgc catgattctc tcgctgctct acaagagcac ggccgaacat
     3481 gtgcggctca tcatgatcga cccgaagatg ctcgagcttt cggtctacga aggcatcccg
     3541 cacctgctcg cgcccgtggt cacggacatg aaacacgccg ccaatgcgtt gcggtggtgt
     3601 gtcgcggaga tggaacggcg ttacgcgctc atggccgcgc tcggcgtgcg caatatctcg
     3661 gggttcaacc gcaaggtcaa ggacgccacc gaggccggca aacccatccg cgatcccgta
     3721 atgacggctg ccgcggccaa tgatcccacc atcgaccaga ggctcatcca ggacctcgtg
     3781 ccgctgccat acatcgtcgt cgtcatcgac gagctggccg acatgatgat gatcgtgggc
     3841 aagaaggtcg aagagctgat cgccaggctg gcgcaaaaag cccgcgcgag cggggtgcac
     3901 ctgatcctcg cgacccagcg cccctcggtc gacgtgatca ccggtctcat caaggccaac
     3961 attccctgcc gcatcgcctt ccaggtgtcg gcgcgcgtcg actcgcgcac catcctcgac
     4021 cagatgggcg cggaatcctt gttaggccac ggcgacatgc tctacctgca gtcgggtacc
     4081 tcggtgccga cgcgcgtgca cggcgcgttc gtcagcgatg ccgaagtgca tcgcgtggtc
     4141 gaatcgctga agcggagtgg cgcgccgaac tacatcgagg acgtgctcga aggcccgagc
     4201 gtgcctctcg ccgggatctc cggcgagccg caggcggagg gtggcgacgc gggcggcgag
     4261 caggatgcgt tgtacgacga ggccgtgaaa atcgtcacca ccgagcgcaa gccttccatc
     4321 tcctacgtcc agcgccggct caagatcggc tacaaccgcg ccgcgcgcct gctcgagtcg
     4381 atggagaacg caggtgtcgt aggcccgctg cagccgaatg gcgcgcgcga aattctcgta
     4441 ccggctcctc caggaagtga atgagtaagc gaacgatgcg agtggcagga ctgctggcaa
     4501 gcctgggcat gaccggcgcc gtgcaggcgc agactccgct cgacagctac ctcgagcatc
     4561 tcaagactct gcgcgccgag ttctcgcagg tggtcaccga tggcaaggga cgtgaagtcc
     4621 agaacgcgcg gggcaagttg accatcgtgc gccccggcag attccgctgg gagctcacgc
     4681 catcggttgg cacgagcagt tcaccgcagc tcatggtcgc cgacggcaag aacctgtggt
     4741 tctacgatcg cgatctcgag caggtttcgg tcaaacccgc gtcgagcgcc ctcacggcca
     4801 cgcccgccgg cttgctgtcg ggcgagggga acattcgtga gctgttcacg gttgcgccgg
     4861 ccggccggaa agacggactg gactgggtgc tggtgacgcc aaaggaaaac gacgcggatt
     4921 tccgcgaagc gcggctggcc ttcggccctg gtccacgaaa agaaggtgag ctcaaacgca
     4981 tggtgctcaa ggacaaactg ggtcagaccg tgcgcctcga tttcgatacc agcgagcgca
     5041 atccgccggt cgccgaagcg gaagtgaagt tcatcccgcc ggcggatgcg gacgtcatcg
     5101 gcacgccggc caaatgaact tgcagcgcgt gtggtggacg ctcggcgtgg tgttcgtcgt
     5161 cctggcgatg gtcgtgtgcc tggtgccggc tccccaggta ccgagcccga tggagtggaa
     5221 cgacaaggtc agccatctcg ctggccatgg gctgctggcg gtctacttcg ctggcctggt
     5281 tccgcggcgc agctggtgga agatattcct gttcctgttg gtgttcggcg ctgtcatcga
     5341 atttgcccag tactacacgc acctgggtcg cgagggagat ccacgggatg tgctggcgaa
     5401 ctccgccggc gcggtgctcg gcctggtcgc ggcgcgcctg gggctcgcgc gctggccgga
     5461 tctcgcggca tggttgttag ggcggcgggc cacgcaatga gttcgcgcgg cgccggcaat
     5521 ccgggcgggc agcccgattt cctgcgcgac gtcccatccg acgatgcgtt gcgtccgctc
     5581 gcggaccgca tgcggccacg caccctcgat gaattcgtcg gccaggagca ggtgctgggg
     5641 tccgggaagc cgctgcgcca ggcactggaa aaaggccagc tccactcgtt gatcctgtgg
     5701 ggtccgccgg gcaccggcaa gactacgctg gcgcggctca tcgcgcgccg cgccgacgcg
     5761 gagttcatgc agctctcggc ggtcatggct ggcgtcaagg acatccgcga atccgtcgag
     5821 cgcgcgcgca cggcgcgcgc cgagcgaggg cgccgcagca tactgttcct cgacgaagta
     5881 caccggttca acaaggcgca acaggacacg ttcctgccgt acgtcgaaga cggcacgctg
     5941 accttcatcg gcgcgaccac cgagaatccc tcattcgagg tcatcagcgc tctgttgtcg
     6001 cgcgcccgcg tgtacgtgct gcgatcgctc ggcgtcgacg acatcgtcaa actactgcgt
     6061 cgcgcgctgg aggattccgg acgcggcctg ggccagcgga acatcgacat cgatgacgaa
     6121 gcgctggtcg cgctggcaca tggtggcgat ggtgatgcac ggcgcgcgct cggaatgctg
     6181 gaagtggcgg ccgacctcgc cactgaacat gagggccgtg caaggatcac gatagagcag
     6241 gtgcgtgaag tcgccaccgg cagccgtcgc cggttcgaca agggtggcga gcagttctac
     6301 gaccagatct cggcgctaca caaggcggtg cgcggcacgg atccggacgc cgcgttgtac
     6361 tggttcgccc gcatgctcga cggcggctgc gaccctcact acctggcgcg gcgcatcgtg
     6421 cgcatggccg tggaagacat cgggctcgcc gatccgcgcg ggcagcagct cgcgctcgat
     6481 gcgtggcaga cttttgagcg gctcggcagc cccgagggcg aactcgcgct ggcgaatgcg
     6541 ctggtgtacc tggcggtggc gccgaagagc aacgcggtct acgtggccta tggcgaagcc
     6601 agggccgatg tcgagcaatt cggcacgctc gacgtgccgc tgcgtttccg caatgcgcct
     6661 accaaactca tgaaggggct cgggtacggc gagggctacc aatacgcgca tgacgcgccc
     6721 gacgctttcg tcaagggcga gcgctatctg cccgacgaaa tgccggatcg ccgttactac
     6781 cgtcccgcgc cgcgcgggct cgaaatcaag atcggtgaag cactggcgcg gctgcggaaa
     6841 cccgaagagg acaaatcatg agctggccca cgtgaactgg ttatgggtgg ggattggcgg
     6901 cgcgctggga agcgttgccc gttacggtgc ctatcgcctg tggccgtcgt cgcccggcgg
     6961 ctttccggta cctacgttca ccgtgaatct gctggggtcc ttcgccatcg gactcatata
     7021 tatgtatgtt gcggcccggg gcgccagcgc cgaaaatgcg cggcttttct ggatgaccgg
     7081 cgtgctcggc gggttcacta cctattcggc gtttgcgctg gaaaccgcgt tgctggggtt
     7141 ttcgtggacc ggcgtggcct atgtgggcgc gacggtcttc ggttgcctgg ccgccgcctt
     7201 cgccggattg aagatgggga cattcctcgt ttcgtgagag agaaagactt tgctcgacgc
     7261 caaacaactt cgcgctgatc ccgccgcagt cgccgccaat ctcgcgcgcc gcggattcac
     7321 cttggacgtg ggtgctttta cgattctcga cgagcttcgt aagcaggcac aaatcgaatc
     7381 cgaccgcgta cgcgccgaac gcaacgcgaa cgccaagtcg gtcggcatgg ccaagggcaa
     7441 gggcgaagat gccagtgcat tgctggcccg cgcggaagaa cttaccaaca agctcgcgtc
     7501 cagcgatgcc gccatcgcca gtgtccagca gcaactcgac gcctggcagt tgggtctgcc
     7561 caatacgttg cacgcttcgg tccccgacgg gcgcgatgaa tccgccaacg tcgaaatccg
     7621 caggatcggc gagccgcgaa aattcgattt cgagccgaag gatcacgtcg agctcggcga
     7681 aaaactcggt ggcctcgatt tcgaaaccgc ggcgaagatt tccggggcgc gtttctccgt
     7741 catgcgcggc ggcctcgcac ggctgcatcg cgcgttggcg cagttcatgc tcgacctgca
     7801 cacacgcgac catggctaca ccgagttgta cgtgccgtac ctcgtgaatg cttccacgct
     7861 gcagggcacg ggccagttgc cgaagttcga agcggatctg ttccgcgtgc agggcgagca
     7921 gggcttctac ctgatcccca ccgccgaagt gcccgcgacc aacctcgtgc gtgacagcat
     7981 cgtcgacccc aaacaactgc cgatgaggtt cgtggcgcat acgccgtgct tccgttcgga
     8041 agcgggcgcc gcgggcaagg acacgcgcgg gctcatccgc cagcatcagt tcgagaaggt
     8101 cgagctggtg cacatcgtca aacccgccga ctcgtacgac gcgctcgaag agctcacgag
     8161 tcacgccgaa caggttctgc agagactcga gttgccgtac cgtgtaatcg cgctgtgcgc
     8221 gggtgacgtc ggtttctccg ccgccaagac ctacgacatc gaagtgtggc tgccggcgca
     8281 gaacaaatat cgcgagattt catcctgcag caactgcgaa ggcttccagg cgcgtcgcat
     8341 gcaggctcgc tggcgcaatc cggataccgg caagcccgag cccctgcaca cgctcaacgg
     8401 ttcgggtgtt gcggtgggtc gcgcactggt cgcggtgctc gagaattttc agaacgcgga
     8461 cgggtcgatc accgtgcccg acgcgctgcg cccctacatg gacggaatgg ggacaatccc
     8521 catttcctag caaaaggggc cgcacctcat gaacgtgggt gtcccgaaag cactgagtct
     8581 tgcgctccgc atgcaggagc tgaaccgttg gctttcggcg caggaaggct cggctggaga
     8641 gacccatgag tttttcgcga tgccccatgg caacgcactc gtcacgatcg atcccacgag
     8701 tgaggcgccg gccgcgtcgt acaaccgcaa tcgaatcgcc ctctgcggtg cgcgcggtgg
     8761 cctgacgcag gacggtttgc gtgagttgct cgacatgttc gctagtcgcc gcatcccgcg
     8821 cgtcttcgcc tggctgagcc ccggccccga cgacgcgctg gtgcgggatt ggctccacga
     8881 attgtcgttc acgcgcgttc cgtggacgcg atatccgacc atgatgttca gcgccatcgc
     8941 cgacgagttt cgcaggaccc agttcgagat ccgccaggtc gaaatggcgg agtttcccgc
     9001 cttacgcgac gagctggatg atgtcttgat ggaagggtac gaacacacgc tggggaaacc
     9061 cggcttccac cactacctgc tgttcgatga ggggcggccg attgcgctcg ccgcactcgt
     9121 gaagttcggc gagatcggct atctgaccta cgcaggcacg atcgaacggg aacgccggc
//
LOCUS       DASQJS010000196         5802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1994710, whole genome shotgun sequence.
ACCESSION   DASQJS010000196 DASQJS010000000
VERSION     DASQJS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994710"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VEW08_08045"
     CDS             complement(<1..277)
                     /locus_tag="VEW08_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYJ40726.1"
                     /translation="MKTPDVINALGALAHEYRLAIYRILVEQGPAGLPAGVIGERVGL
                     VPSSLTFHLQSLQRAGLITQLRASRQLIYSADFGLMNELVGYLTDKCC"
     gene            complement(358..1182)
                     /locus_tag="VEW08_08050"
     CDS             complement(358..1182)
                     /locus_tag="VEW08_08050"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HYJ40727.1"
                     /translation="MADTLATIEQVRSTAVDLAMKFGPKVLAALLILTLGYFVGRWIS
                     RWLERGLGHIEMEPPVRTLIVRIARAVISVLFVIMALQNLGVELLPLIAGLGVAGAGI
                     ALAMQGVLGNLFAGLTIILTKPFRIGEYIEIVSEEGAVESISLFQTTLTHPDRSRVVI
                     PNRKIVGEILHNYGRIRQVNVVVGVAYDTDLNRAIALIQETLRANGRILQEIEPLIVV
                     SLLADSSVNIAIKPWVEIKDFGVAASEINQAILETFRGARIVIPFPQREVRMVAAA"
     gene            1275..1721
                     /locus_tag="VEW08_08055"
     CDS             1275..1721
                     /locus_tag="VEW08_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40728.1"
                     /translation="MARILFPHLAREHGNMKNHFHALVWIDHQTAKVFHFDADSNETD
                     IVHSTHPHQHLHHKANSGDSGHAPVDKDFLERVATAISHSGAILIVGPASAKSELHTH
                     LKNAHPAIAAKISAVESIDHPTDGQLLAHARHFFKADDRMRSQIKS"
     gene            1795..3348
                     /locus_tag="VEW08_08060"
     CDS             1795..3348
                     /locus_tag="VEW08_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ40729.1"
                     /translation="MQSSPTDARRDLTVMLSSSLVRSVLDSLPDAIVIIDSTGNVLFA
                     NHQVEALFGFSSSEIVGGPVEVLLPERFRHRHVAHRKGYTGNVRVRPMGAGLDLFATR
                     KDGTEFPVEISLSPVTFGDDILVAAAIRDVTDRKQVEQALKEARREADHANLAKSRFL
                     ATASHDLRQPLQTLGLLNGALRRMVTDGECREVIDQQELAVDAMSRLINALLDISKLE
                     SGAIKLELTAFEIGTLFEELRGEFAGLANSKGLRFSTDSPGELVYSDPALVGQVLRNL
                     VSNAIKYTHAGSVELRCEHRGPQLRIEIRDTGVGIAAEQLPHIFEEFYQVGVSPNSSR
                     EGYGLGLSIVQRIARLLDMKVEVNSVLRAGSVFSVELPVAQNADRLPAASESKSAPDA
                     VAGGTFNLLLVEDEPSVLNAMRILLTIEGYRVVTAPTAAEALELLRDGVDFDLIITDY
                     HLEAGRTGTQVISVAREMLGDSLKAILVTGDTSSAVRELQGDDNLRITSKPINSSELL
                     GLVKTLLAG"
     gene            complement(3304..4161)
                     /locus_tag="VEW08_08065"
     CDS             complement(3304..4161)
                     /locus_tag="VEW08_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYJ40730.1"
                     /translation="MEKLTRILAVAQGIEEGATVLGKSVTLARRFGAHVELLMLGNVD
                     AQELAKLCADAGYDKLTLASLHPDAESLTEVILRRVFATQPDLVVKSPAGQHPLNRWT
                     LDENDWHLANECPAPVLLVRHNPWARPIRFAAAIDVADIESADTARSILHAAGFLALG
                     CLGGLDILYSEREQHDDALRMERAVKLAQLVREYHVGCERIQVFNGAPEQTLPPLVAA
                     RHYDVLVLGAKSRQPLLKNIFGATNSRLMQATEGDVVLVKAPDHGAGIGQRRDSPREK
                     RFHETEQFA"
     gene            complement(4165..4512)
                     /locus_tag="VEW08_08070"
     CDS             complement(4165..4512)
                     /locus_tag="VEW08_08070"
                     /inference="COORDINATES: protein motif:HMM:NF045308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HYJ40731.1"
                     /translation="MHTMIDDRLRGAREWLLARSAELRERLSRVHDDLSRESTPLPRD
                     APDAAIVMENDEILRAIDETARNELNQIGRALERIGAGTYGLCEGCGGSIDAERLRVV
                     PYAANCRSCAPDA"
     gene            4622..>5802
                     /locus_tag="VEW08_08075"
     CDS             4622..>5802
                     /locus_tag="VEW08_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYJ40732.1"
                     /translation="MFPRETQALMEAAVDAIIVIDHRGRMQAVNEAASRMFGYRSDEL
                     LGENVSVLMPEPDRSSHDAYLAEHLKTGVAKIIGLGRAVTAQRKEGTLFPARLSVGRI
                     PDSNPPRFVGVVRDTTSEHAATAELKLERDRANAYLELHDSILVTLDAGRRVREVNTR
                     GAELLGAPAQDILGSEWLDYMRGDGERECARLLLESALANGSSREREFDAVIANGEPR
                     TIYWRCIARRAADGTPAGWLCSGVDMTDRVQREEQAMLAQSRMTQVARLATMGEMAAG
                     LAHELNQPLTAITTYARACERFIDMPVPDYAELRDSVREIAAEGLRAGAIINRLRKLV
                     RNDEQEEHVPADINAIIDELKVLMSADARTFDTRLHITLAPGLPKVSANVAQLQQLVL
                     NL"
BASE COUNT         1127 a         1826 c         1759 g         1090 t
ORIGIN      
        1 cacaacactt gtcggttaag tagcccacca gctcattcat gaggccgaag tcagcgctgt
       61 agatgagttg ccggctggcg cgcagctgcg tgatcaaccc ggccctttgc aatgactgca
      121 gatgaaacgt cagcgacgat ggcacgagcc ccacacgctc gccgatgacg ccggcgggca
      181 atcctgccgg gccctgctcc accaggatcc ggtagatggc aaggcggtat tcgtgggcga
      241 gcgcacccag ggcattgatg acgtccggtg ttttcatcgg gtcgcacgat attacgacta
      301 ctatcgaaac gtcaagcgat cgtctcccgt ttccagtgga acggctccct tctcgattca
      361 ggcggcggca accattcgaa cctcgcgctg gggaaacgga atgacgatgc gggcgccgcg
      421 gaaagtttcg aggatggcct ggttgatctc actcgcagcg accccgaagt ccttgatctc
      481 cacccagggt ttgatggcaa tgttcacgga cgaatctgcg agcaggctca cgactatcag
      541 cggttcgatc tcttgcagga ttcgaccgtt tgcacgcagc gtttcctgaa tcagtgcgat
      601 ggcgcgattc agatccgtgt cgtaggcaac gccgaccacg acattgacct gccgaatcct
      661 gccgtagttg tgcaggatct caccgacgat cttgcgattg ggaatcacca ctcgcgagcg
      721 gtccggatgc gtgagcgttg tctggaacaa cgagatgctc tccacggcgc cctcctcgct
      781 gacgatctcg atgtactcgc caatccggaa tggcttcgtg aggatgatgg tcagaccggc
      841 aaagagattg ccgaggacac cctgcattgc caatgcgatc ccggcacccg cgacaccgag
      901 cccggcaatc agcggcaaca gctcaacgcc gaggttttgc agcgccatga tcacaaacag
      961 caccgaaatc acggcgcggg ctatccgcac gatcaaagtg cgaaccggcg gctccatttc
     1021 gatgtggcca aggccgcgtt cgagccatcg cgagatccag cgtccgacga agtaacccag
     1081 ggtcaggatc agcagggcgg cgagcacctt gggcccgaat ttcatggcaa ggtccactgc
     1141 cgtgctgcga acttgctcga tggttgccag tgtgtcggcc atgacggcgg tctccaattg
     1201 cccggggctt gaaccctaca acgctttaga gggcgcgtca gccggccggg cttctcgcta
     1261 cgcctgagta cgtgatggcc cgtatccttt ttccgcatct cgcgcgcgaa catggcaaca
     1321 tgaaaaacca tttccacgcc ctcgtctgga tcgatcatca gacggcaaaa gtgtttcact
     1381 tcgatgccga ctccaacgag acagacatcg tccacagcac tcatccacat cagcacctgc
     1441 atcacaaggc gaattcaggt gacagcgggc atgcgcccgt ggacaaggac ttcctcgagc
     1501 gagtggccac ggccatttct cattcgggag ccatcctgat cgtgggaccg gccagcgcga
     1561 aatccgagct ccacacgcac ctcaaaaatg cgcatccggc catcgctgcg aaaatttccg
     1621 cggttgaatc gatagaccac ccgaccgatg ggcagctgct cgcacacgcg cgtcacttct
     1681 tcaaggccga cgaccgcatg cgctctcaaa taaagagctg acgtcgcccc acaggtattg
     1741 aggcccggcc accaggtggc gagcggcgcc gctacttgcg ccagcccatt cgcagtgcaa
     1801 tcctcgccca ccgatgcccg gcgcgacctg actgtgatgc tttcttcttc gctcgtccga
     1861 agtgtgctgg attcactgcc ggacgcgatc gtcatcatcg actcgacagg aaatgtcctg
     1921 ttcgcaaacc accaggttga agcgctgttc ggcttttcca gttcggaaat agtcggtgga
     1981 ccggtcgaag tcttgttgcc ggagcgcttc cggcaccgcc acgtggcgca tcgcaagggt
     2041 tacaccggca acgtacgggt tcgccccatg ggagctggcc tcgatctgtt tgccactcgc
     2101 aaggacggca ccgagtttcc ggtggagatc agcctgagcc cggtcacgtt cggcgacgac
     2161 atcctcgtgg ccgcggcgat acgcgacgtt accgatcgca aacaagtgga acaggcgctg
     2221 aaggaggccc gacgcgaagc cgaccatgcc aacctcgcca agagccggtt cctggccacc
     2281 gccagtcacg atcttcgcca gccattacag acgctgggtt tgttgaatgg cgcgcttcgg
     2341 cgcatggtca ccgatggtga atgtcgcgaa gtgatcgatc agcaggaact ggccgtcgat
     2401 gcgatgtccc gtttgatcaa cgcactgctc gatatcagca agctggaatc cggcgccatc
     2461 aaactggagc tcactgcctt cgaaatcggc acgctgtttg aggaattgcg tggagaattc
     2521 gccggacttg ccaacagcaa ggggctccgg ttttccacgg actcaccggg tgagctcgtg
     2581 tattccgatc cggccctcgt tggccaggtg ctgcgcaatc tcgtctccaa cgcgatcaaa
     2641 tacacgcatg ccggctcggt agagcttcgc tgcgaacacc gcgggccaca gctgcgcatt
     2701 gaaatacgcg acaccggcgt cggcatcgcc gccgagcaat tgccgcacat tttcgaagag
     2761 ttctaccagg tcggggtctc gccaaacagt tcgcgggagg gttatgggct tggcctttcc
     2821 atcgtgcagc gcattgcccg tctgctcgac atgaaagtag aagtgaattc ggtactgcgt
     2881 gcgggctcgg tattttcagt cgaattgccg gtcgcgcaaa atgcagaccg gttgcccgcg
     2941 gcttcagagt cgaaatcagc gccggacgcc gttgccggcg gcacgttcaa cctgctgctg
     3001 gtggaggacg agccgagtgt gctcaatgcg atgcgcatcc tgctcaccat cgaaggttat
     3061 cgcgtggtga ccgcgcccac cgccgccgaa gctctcgagc tgctgcgcga cggggtcgac
     3121 ttcgatctca tcatcacgga ttatcatctc gaggcaggtc gcacgggcac gcaggtgatt
     3181 tcggtcgcgc gcgagatgct cggcgattcg ctcaaggcca tcctggtcac gggcgacacc
     3241 tcttccgccg ttcgcgaatt gcaaggtgac gacaatctgc gcatcaccag caaacccatc
     3301 aactcaagcg aattgctcgg tctcgtgaaa acgcttctcg cggggtgagt ccctgcgctg
     3361 accgatgccg gcaccatgat ccggtgcctt taccagcacc acgtctcctt cagtcgcctg
     3421 catcagtctg ctgttcgtag cgccgaaaat gtttttcagg aggggctggc gcgattttgc
     3481 gccgagcacc agtacgtcgt agtgccgcgc cgccacgaga ggcggcagcg tctgctcagg
     3541 cgcgccattg aacacctgga tgcgttcgca gccgacatga tattcacgta ccagctgggc
     3601 gagtttcacc gccctctcca tgcgcagtgc atcgtcgtgc tgctcgcgtt cgctgtagag
     3661 aatgtccaga ccgccgagac agccgagcgc caggaaaccc gcggcgtgca ggatgctgcg
     3721 cgcagtgtcg gcggactcta tgtcggcaac gtcgatggct gccgcgaagc ggatcgggcg
     3781 agcccatggg ttgtggcgaa ccaggagcac gggcgccggg cattcattcg ccaggtgcca
     3841 gtcattttca tccagcgtcc agcggttcaa cggatgctgg ccggcagggg atttgaccac
     3901 gagatccggc tgcgtcgcga acacgcggcg caggattacc tcggtcagtg attcggcatc
     3961 gggatgcagg ctggccaatg tgagcttgtc atagccagcg tccgcgcaca gttttgccag
     4021 ctcttgagca tccacgttgc ctaacatcag aagttctaca tgcgcgccga agcgccgcgc
     4081 gagtgtcacc gacttgccga gcacggtggc gccctcttca atgccctgtg cgactgcgag
     4141 gattcgggtg agtttttcca tggctcaggc gtccggcgca cagctgcggc aattcgcggc
     4201 gtagggcacc acgcgcagtc tctcggcgtc gatactcccg ccacagccct cgcaaagccc
     4261 gtacgtgcct gccccgatcc gctcgagcgc gcggccgatc tggttgagtt cattacgcgc
     4321 ggtctcatcg attgcccgca ggatctcgtc gttttccatg actattgcgg cgtcgggtgc
     4381 atcgcgcggc agcggtgtgg actcgcggct cagatcgtca tggacgcggc tcagacgctc
     4441 gcgcaactct gcgctgcgcg ccaggagcca ctcgcgagcg cctctcagcc ggtcgtcgat
     4501 catggtgtgc atggaagcga tcgtggcata ggcgtgtggc ggctcacatt cgctcgtata
     4561 cctatgatgt cagtggtatt gcgtactccc gcgcccgatg tgcgccgcca tactgcggtc
     4621 aatgttcccg cgtgaaaccc aggcgctcat ggaagcggcg gttgatgcca tcatcgtcat
     4681 cgaccatcgc ggccggatgc aggccgtcaa cgaagccgcg agccgcatgt tcggctatcg
     4741 gtccgatgag ctgctcggtg aaaacgtgag cgtgctcatg cccgagcccg atcgcagcag
     4801 ccacgacgcc tacctggccg aacacctgaa aaccggcgtc gcgaaaatca tcggcctggg
     4861 acgggcggtg acggcgcagc gaaaggaagg cactcttttt cccgcgcggc tctcggtggg
     4921 acgtatcccg gacagcaacc cgccgcggtt cgtgggcgtc gtgcgcgata ccacctcgga
     4981 gcacgcggca acggcggaac tcaaactcga acgggatcgc gccaacgcat acctggaact
     5041 gcacgattca atcctggtga cgctcgacgc cgggcgccgc gtgcgggaag tgaatacgcg
     5101 cggagccgag ctgctgggcg ctccggccca ggacattctc ggcagcgaat ggctcgacta
     5161 catgcgcggt gacggcgaac gcgaatgcgc acgcctgttg ctcgagagcg cgctcgcgaa
     5221 cggcagctcg cgcgagcggg agttcgatgc ggtcatcgcc aatggcgagc cgcgtacgat
     5281 ctactggcgc tgcattgcgc gtcgcgccgc cgatggcaca ccggcaggct ggctgtgttc
     5341 gggcgtggat atgaccgacc gggtgcaacg cgaggagcaa gcgatgctgg cgcagagccg
     5401 catgacgcaa gtggcgcgcc tcgccaccat gggcgaaatg gcggccggac tcgctcacga
     5461 gctcaaccag ccactgacgg cgatcaccac ctatgcgcgc gcctgcgaac ggtttatcga
     5521 catgccggta cccgactacg ccgagctgcg cgactcggtg cgcgaaatcg cggccgaggg
     5581 actgcgtgcc ggcgccatca tcaaccggtt gcggaagctg gtgcgcaacg acgagcagga
     5641 agaacacgtt cccgccgaca tcaatgcaat catcgatgag ttgaaggtgc tcatgtcagc
     5701 tgatgcacgt acatttgaca cgcgcctgca catcacgctg gcaccgggcc tgccgaaagt
     5761 cagcgcaaat gtggcacagc tgcagcagct ggttctcaac ct
//
LOCUS       DASQJS010000197         4929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_110234, whole genome shotgun sequence.
ACCESSION   DASQJS010000197 DASQJS010000000
VERSION     DASQJS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110234"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VEW08_08080"
     CDS             <1..428
                     /locus_tag="VEW08_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HYJ40733.1"
                     /translation="GDFHAARDLLGRPYSMRGRVVTGEKLGRRLGFPTANIRMRRHKL
                     PFTGVYAVRVRGIDGNLPRDAVASLGFRPTVDGTEPLLEVHVFDFSGWLYGRELEVDF
                     VAKIRDEEKFDSLDALVRHMRQDAIVARGLLLKSHAPQG"
     gene            447..3284
                     /gene="ileS"
                     /locus_tag="VEW08_08085"
     CDS             447..3284
                     /gene="ileS"
                     /locus_tag="VEW08_08085"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HYJ40734.1"
                     /translation="MADYKNTINLPETGFPMKADLAQREPKMLAAWDQNDLYGKLRAI
                     ARGRPRFVLHDGPPYANGALHLGHAVNKVLKDIVVKSHTLDGYDSPYVPGWDCHGLPI
                     EHQVEKKHGRVGAKISAKDFRAACRKYAHEQVDLQRSEFKRFGVLGDWNRPYLTMDPR
                     FEAQQIRALGHIIRNGHLYKGAKPVHWCLDCRSALAEAEVEYEDKTSSAVDVEFRVVD
                     NTELARRVGVNPDVLGAFPVSIPIWTTTPWTLPANEAVSLGPKIEYVLLWVSRNGSSE
                     LLVLANELLDACLARYGLGKGQVLVQFEGAALEHLQLAHPFLPKHVPVIVGEHVTLEA
                     GTGAVHTAPAHGQDDFVIGQKYSLPVDNPVMGDGRFREGTPFVAGLKVDEGGELLIKE
                     LESRGRLLKHEQIKHSYPHCWRHKTPVIFRATPQWFISMDQKGLRANALRDIKKVQWT
                     PGWGEQRIFGMIEKRPDWCLSRQRTWGVPIALFVNKQTQELHPRTAELIEQVATRVEK
                     DGIDAWFEMEAAELLGAEAETYEKVTDVMDVWADSGLTHECVHHTHPDDVKPPVDLYL
                     EGSDQHRGWFHSSLLMSEALYARAPYKGVLTHGFTVDDKGRKMSKSLGNVILPQKVMS
                     TLGADVMRLWIAATDYANEMGVSDEILKRISDSYRRMRNTVRFLLGNLAGFDPAADAL
                     PVEQMVAIDRWALARTAALQEEVVEAYRKYEYHLIYQKIHNFCVVDLGGFYLDILKDR
                     LYTTPKKSVARRSAQTALFHIAESMVRWLAPILSFTSEEIWSFMPGKRGESVFFETWH
                     ALPKVDAGALDWNALIALKTAVGQELEKLRVAGTIGSSNDAEVEVFCKDEFLERFKSL
                     GEELRFLLIVSSAQVKRVSNAAGPPVGAIKVAEVAKEGGLWIRVQVTPAPKCERCWHH
                     RPEVGSNAVHPTLCARCVDNLDGPGESRTFV"
     gene            3305..3805
                     /gene="lspA"
                     /locus_tag="VEW08_08090"
     CDS             3305..3805
                     /gene="lspA"
                     /locus_tag="VEW08_08090"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HYJ40735.1"
                     /translation="MHIPVSASGIRWLWISVLVIAVDQLTKLWIERTMVLGDSFVVLP
                     VLDIVRAHNTGAAFSFLADAGGWQRWAFSLLAVGVSIALVVWLRRLALLTHALLAFGL
                     SLILGGAIGNVIDRFEHGYVVDFVHAHWGSAYFPAFNVADAAISVGAALVILDAILEG
                     RRKKGT"
     gene            3802..4743
                     /gene="ispH"
                     /locus_tag="VEW08_08095"
                     /gene_synonym="lytB"
     CDS             3802..4743
                     /gene="ispH"
                     /locus_tag="VEW08_08095"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HYJ40736.1"
                     /translation="MKILLANPRGFCAGVDRAIDIVERAIDLFGAPIYVRHEVVHNVH
                     VVERLRQLGAVFVDELDQVPDSATVIFSAHGVPAAVENEAARRGLTVFDATCPLVTKV
                     HMEVARYAREGRDVVLIGHAGHPEVEGTMGRFDAGNGGRMHLVENVEQAAKLEVRDPE
                     KLAFVTQTTLSVDDTAAIVGALRERFPSLASPRREDICYATQNRQDAVKELIANCDVL
                     LVVGSITSSNSNRLRELAERAGRPGYLIDDASFLKREWFEGKGAVGVTAGASAPEELV
                     QQVVARLREWGGTTASELVGREEHVVFSLPRALRGRS"
BASE COUNT          889 a         1528 c         1640 g          872 t
ORIGIN      
        1 ccggggattt tcacgcggcg cgcgacttgt taggccggcc gtattcgatg cgcgggcggg
       61 tcgtcactgg cgaaaaactg ggccgcaggc tcgggtttcc gaccgcgaac atccgcatgc
      121 gccggcacaa gctgccgttt accggggttt acgcggtgcg cgtgcgcggc attgacggta
      181 atctcccacg cgacgccgtg gcgagcctcg ggtttcggcc cacggtggac gggacggaac
      241 cgctgctcga agtgcacgta ttcgatttct ccggctggct gtacggccgc gagctggaag
      301 tggatttcgt cgcgaagatt cgcgacgagg aaaaattcga cagcctcgat gcgctggtgc
      361 ggcacatgcg ccaggacgcg atcgtggctc ggggcctgct cttgaagagc catgccccac
      421 aaggttagtt agaggaagag aactcagtgg cggactacaa aaacaccatc aatctccccg
      481 agaccggttt tccgatgaag gccgatctcg cgcaacgcga gccgaaaatg ctggccgcgt
      541 gggaccagaa cgacctgtac ggaaagctgc gcgccatcgc tcgcgggcgt ccgcgcttcg
      601 tgctgcacga cggaccgccg tacgccaacg gcgcgctgca cctcgggcac gccgtgaaca
      661 aggtgctcaa ggacatcgtc gtgaagtcgc acacgctcga cgggtatgac tcaccgtacg
      721 tgccgggctg ggactgccac ggtctgccca tcgaacacca ggtggaaaag aagcacggtc
      781 gtgtcggcgc gaagatcagc gcgaaggatt tccgtgccgc ctgccgcaag tacgcgcatg
      841 agcaggtgga cctgcagcgc agcgagttca agcgcttcgg cgtgctgggt gactggaatc
      901 gtccgtacct cacgatggac ccccgattcg aggcccagca gatccgcgcg ctggggcaca
      961 tcatcaggaa cggacactta tataagggtg ccaagccggt ccactggtgc ctcgactgcc
     1021 gttcggcgct cgccgaagca gaagtggaat atgaggacaa gacctcgtcc gccgtggacg
     1081 tggaattccg ggtggtcgac aacaccgagc tggcgcggcg cgtcggcgtg aatccggacg
     1141 tgctcggcgc gtttccggtg tccatcccga tctggaccac cacgccctgg acgctgcccg
     1201 cgaacgaagc ggtgagcctt ggtcccaaga tcgaatacgt gctgctctgg gtatcgcgca
     1261 acggctcgtc cgagttgttg gtgctcgcca atgagctgct cgatgcctgc ctggcgcgtt
     1321 acggcctcgg caagggccag gtgctggtgc agttcgaggg cgccgcgctc gagcatttgc
     1381 aactggcgca tcctttcctg cccaagcacg tgccggtcat cgtcggcgaa cacgtcacgc
     1441 tcgaagccgg taccggcgcc gtgcacaccg cgcctgcgca tggccaggac gacttcgtga
     1501 tcggacagaa atacagcctg ccggtggaca accccgtgat gggcgacggc cgcttccgcg
     1561 agggcacgcc attcgtcgcc gggctgaagg tggacgaggg tggggagttg ctcatcaagg
     1621 agctcgaatc ccgcggccgc ctgctcaaac acgaacagat caaacacagt tatccgcatt
     1681 gctggcgcca caagacgccg gtgatcttcc gcgccacgcc gcagtggttc atcagcatgg
     1741 accagaaggg tctgcgcgcg aacgcgctgc gcgacatcaa gaaggtgcag tggacgcccg
     1801 gctggggcga gcagcgcatc ttcggcatga tcgagaaacg tcccgactgg tgtctgtcgc
     1861 gccagcgcac ctggggtgtg cccatcgcgc tgttcgtgaa caagcagacc caggagctgc
     1921 atccgcggac agccgagctc atcgagcagg tggcgacgcg cgtcgagaaa gacggcatcg
     1981 acgcctggtt cgaaatggaa gccgcggagt tgttaggcgc cgaggcggag acctacgaga
     2041 aggtcaccga cgtcatggac gtgtgggccg actcgggtct cacgcacgaa tgcgttcatc
     2101 acacgcatcc cgacgacgtg aaaccgccgg tcgatctgta tctcgagggt tcggatcagc
     2161 atcgcggctg gttccattca tcgctgctga tgtcggaagc cctttacgcg cgcgcgcctt
     2221 acaaaggcgt gctgacgcat ggattcacgg tggacgacaa gggccgcaag atgtccaagt
     2281 cgttgggcaa cgtgatcctg ccgcagaaag tgatgtcgac gctgggcgcc gatgtcatgc
     2341 gcctgtggat cgctgccacg gactacgcca acgaaatggg cgtgtcggat gaaatcctga
     2401 agcgcatttc ggattcgtac cggcgcatgc gcaacaccgt gcgattcctg ttgggcaacc
     2461 tcgctggttt cgatccggcg gccgacgcat tgccggtgga gcagatggtc gccatcgacc
     2521 gatgggcgct cgcgcgcacc gcggcactgc aggaagaagt ggtcgaggcg taccgcaagt
     2581 acgagtacca cctcatttat cagaagatcc acaacttctg cgtggtggat ctcggcggct
     2641 tctatctcga cattctcaag gaccggctgt acaccacgcc gaagaaaagt gtcgcgcgcc
     2701 gctcggcgca gaccgcgctg tttcacatcg ccgagagcat ggtgcggtgg ttagccccga
     2761 tcctgagttt cacgagcgag gagatctgga gcttcatgcc gggaaagcgc ggcgaatccg
     2821 tgttcttcga gacatggcat gcgttgccga aggtcgacgc cggcgcgctc gactggaacg
     2881 cgctcatcgc gctcaagacc gccgtgggcc aggagctcga gaagctgcgc gtcgcgggca
     2941 ccatcggatc gtcgaacgat gccgaggtcg aggtattctg caaggacgag ttcctggagc
     3001 gcttcaagtc gctcggcgaa gaactgcgtt tcctgttgat cgtgtccagc gcgcaggtca
     3061 aacgcgtcag caacgcggcc gggccgcccg tgggcgccat caaggtcgcc gaggtcgcga
     3121 aggaaggcgg gctgtggatt cgcgtccagg taaccccggc gcccaagtgc gaacgttgct
     3181 ggcatcacag gcccgaagtg ggttccaatg ccgtgcatcc aaccctttgc gcgcggtgcg
     3241 tggataatct cgacggaccg ggcgaatccc ggacattcgt atgaaccaaa acgtggacgc
     3301 tgccgtgcac atacccgtta gcgccagcgg cattcgctgg ctgtggatct cggtgctggt
     3361 gatcgcggtc gaccaactca ccaagttgtg gatcgagcgc accatggtgc tgggcgactc
     3421 tttcgtcgtg ttgccggtgc tcgacatcgt gcgcgcgcac aacacgggcg cggccttcag
     3481 cttcctcgcg gatgccggtg gatggcagcg ctgggcgttt tcgttgctcg cggtcggcgt
     3541 ttcgatcgcg ctggtggtct ggctgcggcg gcttgcgctc ctcacccatg cgctgctcgc
     3601 gttcggcctc tcgctgatcc tgggcggcgc catcggcaat gtcatcgacc ggttcgagca
     3661 cggctacgtc gtggacttcg tgcacgcgca ttggggctcc gcctatttcc ccgcgttcaa
     3721 cgtcgccgat gccgccatca gcgtcggcgc cgcgctggtg atcctcgacg ccatcctgga
     3781 aggacggcgc aagaaaggaa catgaagatc ctgctcgcca accctcgcgg gttctgcgcc
     3841 ggcgtggatc gcgccatcga catcgtggag cgcgccatcg acctgttcgg cgcgcctatc
     3901 tacgtgcgcc acgaggtggt gcacaacgtc cacgtggtcg aacggctgcg ccagctcggc
     3961 gcggtcttcg tcgacgagct cgaccaggta cccgatagcg ctacggtcat cttttccgcg
     4021 catggcgtcc ccgccgcggt cgaaaacgaa gccgcgcgcc gtggcctcac cgtattcgat
     4081 gccacgtgcc cgctggtcac caaggtgcac atggaagtcg cgcggtatgc gcgcgaagga
     4141 cgcgacgtgg tgctcatcgg ccacgcgggc catccggaag tcgagggcac catgggccgt
     4201 ttcgacgccg gcaacggcgg ccgcatgcac ctcgtcgaga atgtcgagca ggccgcgaag
     4261 ctcgaggttc gcgatccgga aaaactggcg tttgtcacgc agaccacgct ctccgtcgac
     4321 gataccgccg ccatcgtcgg cgcgctgcgc gagcgcttcc cctcgctcgc cagcccccgt
     4381 cgcgaggaca tctgctacgc cacgcagaat cgccaggacg cggtgaaaga gctcattgcc
     4441 aattgcgacg tgctgctcgt cgtgggctcg atcacgagtt cgaattcaaa ccgcctgcgg
     4501 gagctagccg aacgggccgg gcgacccggt tatctcatcg acgacgcctc tttcctgaag
     4561 cgcgagtggt tcgaagggaa gggcgcggtg ggtgtcaccg cgggcgcctc ggcccccgaa
     4621 gaactcgttc agcaggtggt ggcgcgactg cgtgagtggg gcggcacgac ggcgtccgag
     4681 ctcgtcgggc gcgaagagca cgtggtgttt tcgctgccga gggctttgcg gggaagaagc
     4741 taggaatgga gaggggggtc cagtagatgg gccccagcga gcatcccccg cacaggattt
     4801 cgttcgcttt tcgccgggcc cttcgttcag ggtggcatcg ctggattgcg ggccgccgcc
     4861 acacgcaatg acactttcgt gcggttgtga ggaccggaag cgagagtcct gatgatgcgg
     4921 agaggcggc
//
LOCUS       DASQJS010000198         5649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_110237, whole genome shotgun sequence.
ACCESSION   DASQJS010000198 DASQJS010000000
VERSION     DASQJS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110237"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VEW08_08100"
     CDS             <1..800
                     /locus_tag="VEW08_08100"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HYJ40737.1"
                     /translation="IEARPEYIPRRPSLLPKLLATVNDSEATMAEMAHIIAQDPALTG
                     NLLRIANSPIYRTSSLPVDSIDRAVTLVGVQGIRSIIATALLQPVMTSGSGAAFSRFP
                     EVVWEHTLYAAMAGEAHAVQVENAEPFVSQLIGLLYGLSAIVVFRIVRDQFAAHPHLN
                     PDPGSIARMLETWVAPTAGRIAVSWELSTRVQYALESQTLAAELQMENSLGRSLKFGR
                     VAGALVVLCRLGRVTEQDARAIVVSGEGRRSQVERLWDRLASTYIRK"
     gene            1030..1443
                     /locus_tag="VEW08_08105"
     CDS             1030..1443
                     /locus_tag="VEW08_08105"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HYJ40738.1"
                     /translation="MNQDIQGGCMCGAVRYSATANPANSMICHCQSCRRSAASPVVAW
                     VTFAKAEFRFTRGTPRQYQSSPKVKRHFCGDCGSPLAYEHLDTNDFYDVTTCSLDAPW
                     GFPPTHHSWLSDDIGWVKFGDGLPTFERSRFGDPS"
     gene            1427..1942
                     /locus_tag="VEW08_08110"
     CDS             1427..1942
                     /locus_tag="VEW08_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ40739.1"
                     /translation="MATPPELETSRLTLRRLLFDDAPFLVDLLNQPSFIENIGDRGVR
                     TIEDAHRYLREGPMAMYDRFGFGLWHTALKSGEPLGMCGLLKRDNLPDVDVGFAFLPQ
                     FWGRGFAYEAAAATLRHGAQKFGLTRVVGVVSEGNAGSIRVLEKLGMGFERMVSMREE
                     EPPIRLYGRSL"
     gene            complement(1939..2598)
                     /locus_tag="VEW08_08115"
     CDS             complement(1939..2598)
                     /locus_tag="VEW08_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40740.1"
                     /translation="MKLAAFVVFAFLAFVVWAGSPVSFAADGHNISSVNSSVRGEDGQ
                     TYGKLSTVNGNVRVGRGATADEARTVNGEIVVEDDAKLGEAETVNGSLDVGDGVTVTR
                     TASTVNGSVELGRRTRVGGDVSTVSGEIEINGAEVSGSVITINGDIDLNDGARVAGGI
                     HVKKNKGTNWGWGKDEPIKVHVCGTCVVEGDLRFDRPVELRVDAGAKIGKVIGDSVTR
                     R"
     gene            3082..5379
                     /locus_tag="VEW08_08120"
     CDS             3082..5379
                     /locus_tag="VEW08_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HYJ40741.1"
                     /translation="MALLSTLTAPLAARAESIPRPEGIQSDVNFWIRVYTEVTTNEGL
                     LHDERNLAVVYDTLKFGPGGSSRERQRMVDERRDRHIAALRRIIAALPTEAGREALSA
                     DDKRLLALWGPNPSVIILKDATTRIRFQLGQSDRFKEGLIRSSSWETHIAETFANQGL
                     PPELAVLPHVESSFNAAAYSKVGAAGLWQFMRSTGRRYMRVDDAVDERLDPYRSTEAA
                     AQLLAYNYRVLGSWPLALTAYNHGAAGMRRAKESVGTDDYVKINRTYSSRTFGFASRN
                     FFPSFLAALTIDENPEKYFGALQRRPEQKFREITMPAYVRLATLESTLGVDREQLRVL
                     NPAWRPTIYNGTRLVPRGYRLRLPADTTERWTAEMLSARLPANELYAGQVTPRTHRVR
                     KGETMSAIAQRYGMSAARLGEMNGLSANASLRAGRRLQLPEQLPRTLSGAAPTATVAA
                     AESAPPSPANATAASAPLGDFYVVRRGDSLETISSRVGVPQAQLLTMNALRNPDFLYE
                     GQRLRIAGEAVAVTQAEADVKVAAIDAARGEAQREGAAVQVVREETTRPIGSGEPTRG
                     RARSAAAVAMEAATTPEVATAVVQAAESARQPVSAAQAEELSPALGPVSVTQGLADSI
                     DYQVRDDGSIRVEATETLGQYADWLQIPTQKLRVVNKLKARQPVLLGQKLNLDYSRVS
                     REAFEQVRRDYHAKLQGEFFVQHRIAGTEVYIVRRGDSLWTMTQKFSNLPIWLLRQYN
                     PDTDLSDLRAGTQVVMPRVEVVSGS"
BASE COUNT         1021 a         1799 c         1804 g         1025 t
ORIGIN      
        1 gcatcgaggc gcgacccgaa tacattccgc gacgaccgtc gctgttgccg aagctgctgg
       61 ccaccgtcaa cgacagcgaa gccacgatgg ccgagatggc gcacatcatc gcgcaggatc
      121 ccgcgctcac cggcaacctg ctgcgcatcg cgaacagccc gatctatcgc accagcagcc
      181 tgcccgtgga cagcatcgac cgcgcggtga cactggtcgg ggtgcagggg attcgctcga
      241 tcatcgccac cgcgttgctg caaccggtca tgacatcggg ctcgggggcg gcgttcagca
      301 gattccccga ggtggtctgg gagcacacgc tgtacgccgc gatggccggt gaagcccatg
      361 ccgtccaggt ggagaatgcc gagcccttcg tttcgcagct catcggcctg ttgtacgggc
      421 tgtcggcgat cgtggtcttc cggatcgtgc gcgatcagtt tgcggcgcat ccccatctca
      481 accccgatcc gggaagcatt gcccgcatgc tcgaaacctg ggtggcgccc acggctggac
      541 gcatcgccgt cagctgggag ctgtccaccc gcgtccagta cgcgctggaa agccagaccc
      601 ttgccgccga actccagatg gagaattcgc tggggcgatc cctcaaattc gggcgggtcg
      661 cgggggcact tgtcgtcctg tgccgcctgg ggcgcgtgac cgagcaggac gcccgggcga
      721 tcgtggtttc gggcgagggt cgccgatcgc aggtcgaacg actctgggat cgcctggcct
      781 cgacctatat caggaagtag cccccggacc tttctcgcat ccacgtaata aaggtgactt
      841 aggggtggga gaaaatgcgc cctcgtgaca caaagtattc gtggatgccg gaaccctact
      901 ggtgtccaca gaccagaagc tgcggaacaa gacccccgtt tccgcgcgac tagagccccg
      961 cacaggcggg gctctttttt tagagggcgc aactcacggt aagctgccgc aacaacgggg
     1021 gagaagaaat tgaatcagga catccaggga gggtgcatgt gcggcgccgt gcgctattcc
     1081 gctaccgcca atccggcgaa cagcatgatt tgccactgcc agtcgtgccg ccgttccgcc
     1141 gcgtcaccgg tggtcgcgtg ggtcacgttc gccaaggccg agtttcgatt cacacgtggc
     1201 acgcctcgcc aataccagtc ctcgcccaag gtgaagcgcc acttttgcgg ggactgcggt
     1261 tcgcccctgg cttacgagca cctcgatacc aacgatttct acgatgtgac gacctgcagc
     1321 ctggatgcgc catggggatt tccgcccaca catcattcct ggctcagcga cgacattggc
     1381 tgggtgaaat ttggcgatgg tctgcccacc ttcgagagat cacggtttgg cgacccctcc
     1441 tgaactcgag acatcgcgcc tcacgctgcg gcgcctgctc ttcgatgacg caccattcct
     1501 ggtggacctg ctcaatcaac cctcgttcat cgaaaacatc ggcgatcgcg gcgtgcgcac
     1561 tatcgaagat gcgcatcgtt acctgcggga aggcccgatg gcgatgtacg acaggttcgg
     1621 ctttggcctg tggcacacgg cgctaaagag cggtgagccc ctcggcatgt gcgggctgct
     1681 caagcgcgac aacctgcccg acgtggacgt gggcttcgcg tttctgccgc agttctgggg
     1741 ccgggggttc gcttacgagg ccgctgcggc gacgttgcgc catggcgcgc agaagttcgg
     1801 cctgactcgc gtggtcggcg tggtttccga gggcaacgcc gggtccattc gcgtgctcga
     1861 gaagttaggc atgggcttcg aacgcatggt ttccatgcgt gaagaggagc ccccaatacg
     1921 gctctatggc cgcagcctct agcggcgcgt gaccgaatcg ccgatcacct tgccgatctt
     1981 cgcgcccgca tcgacgcgca gctccaccgg ccgatcgaaa cgcaggtcac cttcgaccac
     2041 gcaggttccg cagacatgca ccttgatggg ctcgtccttg ccccagcccc agttggtgcc
     2101 cttgtttttc tttacgtgaa tgccgccggc gacgcgtgcg ccgtcgttga gatcgatgtc
     2161 gccattgatc gtgatgaccg agcccgaaac ttcggcgccg ttgatttcga tctcaccgga
     2221 caccgtggat acatcgccgc cgacgcgggt gcgcctgccg agttcgacgc tgccattcac
     2281 ggtggaagcc gttcgtgtga cggtgacgcc gtcaccgaca tcgagcgaac cgttcacggt
     2341 ctccgcttca ccgagtttgg cgtcgtcctc gacgacgatc tcaccgttta cggtcctggc
     2401 ctcgtcggcg gttgcgccgc ggccgacgcg gacattgccg ttcaccgtgc tcagtttgcc
     2461 gtaggtctgg ccgtcttcgc ctctgaccga gctgttgacc gaggagatgt tgtgaccgtc
     2521 ggcagcgaac gaaaccgggc ttcccgccca gacgacaaag gcaaggaatg caaagacgac
     2581 gaaagcggca agtttcatgt gcggtccctc gggcgtggtg cctgtgcctc atttgacgcg
     2641 ccgggggcgc gcctggttgc aaccgcgaga agaggacgcc gggaaaaaag aagccccgct
     2701 tgcggcgggg ctcattggtc gcttgcttgc ttgttgtgga ggagtgtttc gctcttcttg
     2761 ttcttgtctg atgcggtctt tgtcgagccg cttgttatta agaagagcaa tcgctgtgcc
     2821 agaggactcc gacatccgcg acgctgccca atcgaccgtg cggggccccg tgggatgcaa
     2881 agttcgagtt tgggcaatat gtcacgtcgg cgccggcacc cctgctgtca aaatcttcga
     2941 cgcatggaag gcgaaggtcc gcgacataac tttgggacaa cgcagcacaa ttgctttgaa
     3001 aacaatggtt tgaataccag ccagcgagtg taaatgagtc cgacagtcca aaaatcatgt
     3061 gcccgagcct ggattcttgc cgtggcgctg ctgtcgacgc tgaccgcgcc acttgcggcg
     3121 cgcgccgaat cgattccgcg tccggagggc attcagtccg acgtgaattt ctggatccgc
     3181 gtctacaccg aagtgaccac caatgaagga ctcctgcacg acgagcgcaa tctcgcggtc
     3241 gtatacgaca cgttgaagtt cggtcccggg ggttcatcgc gcgaacgtca gcgcatggtc
     3301 gacgagcggc gagaccgcca catcgccgcc ctgcgtcgca tcatcgccgc gctgcccacc
     3361 gaagccggac gcgaagccct ctccgcggac gacaagcgcc tgctcgcgct ctggggtccg
     3421 aatcccagcg tcatcattct caaggacgcg actacccgca ttcgctttca gctgggtcag
     3481 tccgaccgct tcaaggaagg tctgatccgc tcctcgagct gggaaaccca cattgcggag
     3541 actttcgcga accagggtct gccgcccgaa ctcgcggtgc tgccgcacgt cgagtcatcg
     3601 ttcaacgccg ccgcctactc gaaggtgggt gccgcgggac tgtggcaatt catgcgttcg
     3661 accggccggc gatacatgcg cgtcgatgac gcggtggacg agcgcctcga cccgtaccgc
     3721 tcgacggaag ccgcagccca actgctggcc tataactatc gggtgctcgg cagctggcca
     3781 ctggcgctca ccgcctacaa ccacggcgcc gccggcatgc gccgcgcgaa ggagtccgtg
     3841 ggcacggacg actacgtcaa gatcaaccgc acctacagca gccggacttt cggattcgcg
     3901 tcgcgcaact tctttccgtc gttcctggcc gcgctgacca tcgatgagaa tcccgaaaaa
     3961 tattttggtg cgctccaacg gcgccccgaa cagaaattcc gcgaaatcac catgccggcg
     4021 tatgtgcgcc tggcaacgct cgagagcacg ctgggtgtgg atcgcgaaca gctgcgcgta
     4081 ctgaaccccg cgtggcgccc gaccatctac aacggtaccc ggctcgtgcc gcgtggctac
     4141 cggctgcggc tgcccgcgga cacgacggaa agatggaccg cggaaatgct gagcgcgaga
     4201 ttgcccgcga acgagttgta tgccgggcag gtcacgccgc gcacgcatcg cgtgcgcaag
     4261 ggcgaaacca tgtcggcgat tgcgcagcgc tacggcatgt cggccgcgcg actcggtgaa
     4321 atgaacggtc tgtctgcgaa cgccagcctg cgcgcaggac gccggctgca actgcccgag
     4381 cagttgccgc ggacactgtc gggcgcagca cccactgcga cggtggccgc ggccgaaagc
     4441 gctccgccgt cacccgcgaa cgcgacagcg gcgagcgcgc cgctgggtga cttctatgtc
     4501 gttcgtcgtg gcgattcgct cgagacaatt tcctcgcgag taggtgtgcc tcaggcgcag
     4561 ctcctcacga tgaacgcgct gcgcaatccg gacttcctgt acgaagggca acgcctgcga
     4621 attgccggcg aagccgtcgc ggtgacgcag gctgaagccg acgtgaaagt cgctgccatc
     4681 gacgccgcgc gcggcgaagc acagcgcgaa ggtgccgcgg tacaagtggt tcgtgaggaa
     4741 accacgcgcc ccattggctc tggagaaccg acccgaggac gggcgcggtc ggctgccgcc
     4801 gtcgccatgg aggccgcgac cacgccggag gttgccaccg cggtggtgca ggcggcggag
     4861 agcgcacgcc agccggtatc ggcggcgcag gccgaagagc tgagcccggc tctcggcccg
     4921 gtctcggtga cgcagggcct ggccgactcg atcgactacc aggtgcgcga cgatggctcg
     4981 atccgcgtcg aagccaccga aactcttggt caatacgccg actggctgca gattcccacg
     5041 cagaagctgc gcgtcgtgaa caagctcaag gcacggcagc ccgtgttgct cggccagaaa
     5101 ctcaacctgg actattcgcg cgtgtcgcgc gaggcgttcg aacaggtgcg gcgcgactat
     5161 cacgccaagc tgcagggcga attcttcgtg cagcaccgaa tcgccggcac cgaggtctac
     5221 atcgtacggc gcggtgactc gctgtggacc atgacccaga aattcagcaa cctgccgatc
     5281 tggctgctgc gccagtacaa cccggacacg gacctttccg atcttcgcgc gggtacgcag
     5341 gtggtgatgc cgcgtgtgga agtcgtatcg ggtagctagg aagagggatg cgggggaggg
     5401 ggtgcgtgcc ccagcgagca gtccgcgcac aggatttcgt tcgcttatcg ctggggtttc
     5461 gtaaagaggg ggcagtttgg gggtgtgcgg gccccagcga gcagtccgcg cacaggattt
     5521 cgttcgcttt tcgccgggcc cttcgttcag ggtggcatcg ctggattgcg ggccgccgcc
     5581 acacgcaatg acactttcgt gcggttgtga ggaccggaag cgagagtcct gatgatgcgg
     5641 agaggcggc
//
LOCUS       DASQJS010000199        20019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2309168, whole genome shotgun sequence.
ACCESSION   DASQJS010000199 DASQJS010000000
VERSION     DASQJS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20019
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309168"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /gene="rplY"
                     /locus_tag="VEW08_08125"
     CDS             complement(<1..247)
                     /gene="rplY"
                     /locus_tag="VEW08_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HYJ40742.1"
                     /translation="MRISFELAAEFRDAEGKGASRRLRHAGRVPAILYGGHAEPRAIT
                     LNHQKLMTLVDNEKFYSSIINLIVDDKKQAAIVKDLQM"
     gene            complement(388..1326)
                     /locus_tag="VEW08_08130"
     CDS             complement(388..1326)
                     /locus_tag="VEW08_08130"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HYJ40743.1"
                     /translation="MTLFAGNANPALAHDIARHLTTPLGRAYVGRFSDGEVNIELMEN
                     VRGRDVFILQPTCPPTNDHLMELLVIVDACRRASAAKITAVVPYFGYSRQDRRPRATR
                     SAITAKLVANMIQSAGVDRLLTIDLHSDQIQGFFDIPVDNVYASPVLLGDAYRQRYEN
                     LIVVSPDVGGVVRARALAKRLDDADLAILDKRRPRPNESKVMNIIGEVRGKTCVLIDD
                     MVDTAGTLAAGAQALKDEGALKVVAYITHAILSGNAIEKISKSAIDELVVTDTIPLST
                     EARQCGRIRQLSVAGLLAETIRRIRDEDSVSSLYLD"
     gene            complement(1418..1492)
                     /locus_tag="VEW08_08135"
     tRNA            complement(1418..1492)
                     /locus_tag="VEW08_08135"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1458..1460),aa:Gln,seq:ttg)
     gene            complement(1498..2346)
                     /gene="ispE"
                     /locus_tag="VEW08_08140"
     CDS             complement(1498..2346)
                     /gene="ispE"
                     /locus_tag="VEW08_08140"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HYJ40744.1"
                     /translation="MVAWSAPAKLNLMLHVVGRRQDGYHELQTVFQLIDLCDRIEITV
                     RTDGSISRPLGAAGVPESEDLVVRAARALQQVSGTRLGAEISVRKRIPLGGGMGGGSS
                     DAATTLLALNQMWNLGYTSEQIGAFGAALGADVPVFAAGRSSWAEGIGEKLTPVSLGA
                     DSWYLVIFPGVSVPTAAVFQAAELTRNSPPTTMRGFLETGGRNDCEAVVRARFPAVAE
                     ALDWLARHGTARLTGTGSCVFAKFSRVEDAERVAARVPDEWRAWVARGLDSSPLLEEL
                     ARCSKS"
     gene            complement(2358..2963)
                     /gene="lolB"
                     /locus_tag="VEW08_08145"
     CDS             complement(2358..2963)
                     /gene="lolB"
                     /locus_tag="VEW08_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HYJ40745.1"
                     /translation="MRGALAATAAMAVLLLSAGCRTLPPSADIGPGADAPWPEQRAAL
                     EKLDRYGLSGRVAVAAQGQGFSASLRYQQQSRSSNLALDGPLGIGGLRVDIEGEGIAI
                     ATSRGEKLDGQAARDELERRLGFQLPLTELRWWLLGLPAPGEVSINQDAGSGEIHDFT
                     QNGWHVSINSRAPGLGFALPQRLTAEREGARLKLLVENWQP"
     gene            complement(2966..4714)
                     /locus_tag="VEW08_08150"
     CDS             complement(2966..4714)
                     /locus_tag="VEW08_08150"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ40746.1"
                     /translation="MPRKTASMLLSLALAALLPAGVMALSTTNSDSADLVLADMALSR
                     GDCRGGTDRYIKAAFASNDASISKRASEIASECQQISASAKAARRWQKLDPESAGASA
                     AVALAAVRLYQPDEASAAILRTQALGGDESLIKLMGEVSDAGGSAIALKTLRPRLDSP
                     EVSERLLAAGVDLALESFDFAAAHKLADRMLSNEPASGTARAQLARVLTAEGDAVAAV
                     AVAQEAAALDPDTERFAYVDTLMRLDRIDEARQELESMREDASIRGEADLRLGKLAYQ
                     MGDLTEAGRRFGSLISSPAEAPEAFFFLSSIAEREGRTDLALEGYSRLAEAGGGLIVR
                     GRAARLLLAKNERDAAFRMLDEHAAKERSEILDVEFAKAALLADVGHSNEAIALLQLA
                     LERFPDHPGVRYQIALIQDKAGMERESVRSFESLLKDRPEDASLLNALGYSLADRNQK
                     LPRAETLIRKALTASPDNPAFLDSLGWVLFRRGDVSGAIPLLERAYRIFPDSEIASHW
                     GEVLWVNGKQSEARTLWARALARAPDSKPLRDTIERLTGTKLEPPAKPKPDSDAPDPD
                     GGPGPILQPPPTSTSS"
     gene            4758..5852
                     /gene="prfA"
                     /locus_tag="VEW08_08155"
     CDS             4758..5852
                     /gene="prfA"
                     /locus_tag="VEW08_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HYJ40747.1"
                     /translation="MKDTIRLRLEKMVDRYEEIGRSLADPAVIGRQREFRELSQEYAR
                     LTPVAEKFGAFQTLERELKSARDMQNDSDPGMREMGQEEMTRLAPEIERAEDALKMLL
                     IPRDPRDDKNIYLEVRAGTGGDEAAIFAGDLFRMYARFAETHGFTVEILSESLGEHGG
                     YREVISRIAGAGAFARFKFESGVHRVQRVPATEAQGRIHTSACTVAILPEPDEVEASD
                     LNPAELRIDTFRASGAGGQHVNKTDSAIRITHLPSGLVVECQDERSQHKNRSRAMALM
                     KAKLMAAEQEKRDSEIARNRKLQVGTGDRSERIRTYNFPQGRVTDHRINLTLYKLGDI
                     MQGKIDELLDSLQHEHQAEQLAEEEQRGAA"
     gene            5849..7357
                     /locus_tag="VEW08_08160"
     CDS             5849..7357
                     /locus_tag="VEW08_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HYJ40748.1"
                     /translation="MNAAARGLEAAPMLGVASATGVDAHLVLAGPGARSLAFLLDWLI
                     RTALAAAYLLLASWLLLGNFHFEIGPDDESLWYLAGALPATLIYLLYHLILEPLMAGR
                     TPGKRLAGLRVLTLEGLVPSAGALITRNVFRLIDSMPVFYVVGLLFVFFGRQHQRLGD
                     IAAGTVLAVERAPFLEKLARWRRDPTAHGEWRAARQRAAALSRRRGTDVDDALAVVDD
                     YRRAAHALAIERQSAPPVRSAEYLEAVYSDLHDTIHRPARRIPLQLLSLMRDRIPADL
                     SVMKVHILSVTLLFIVSVVTGAWLVYSYPDLIALFANPQLIATVERGELWTDGLLNVT
                     PSAVLSVSILTNNIVVSFFAFCSGIVFGLGTFYLIGTNGLSLGAIFAFTTQHDLGGRL
                     FDFIVAHGCVELSCICIAGAAGSYLGEALIRPGERSRSEAFRAAAAEGIRVMLVVTLL
                     LLICGFIEGYVSPDPEVPRWARVTIGVGYWLFMISLLRGYVFGRSRGGEIAT"
     gene            complement(7377..8675)
                     /locus_tag="VEW08_08165"
     CDS             complement(7377..8675)
                     /locus_tag="VEW08_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYJ40749.1"
                     /translation="MHFALRGYLFVALTALLGVAGTWSDVPAFAGAWLLPAFLLLLGL
                     AIEAWYLRGTKVTMAMRYDDRLKLGRQVRGAYAFAHNRGREIVLQYARVLPPALRQTT
                     EVRCITLPPEGELRDPAELLPLRLGAGRFGNVPARLLGRFALAWWARELQQDTAFTVA
                     PDSLPNRGRPVPGESAGETPRRLPGTGVELLQLRDYVGGDALSRIDWKATARRGTLIS
                     REYSEAQHLEILLVIDAGRSSRVRAGMLDRLGLYANVAARLAEYAVSIEDRVGLLVYA
                     DRTLSASIPERGLNAVARLRHQLEILESDRGESNPVAAAIHVRRLLRHRGLVVWLTDL
                     ADPARNDLLMRALKALVPRHQPIVAAPHAAEIERLTADPARAWRDPAVKIAAREHHQR
                     AQLQVMQLRQRGIVVLDEADNKLDKAVLDSYLRLRSRRRI"
     gene            complement(8679..9629)
                     /locus_tag="VEW08_08170"
     CDS             complement(8679..9629)
                     /locus_tag="VEW08_08170"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYJ40750.1"
                     /translation="MNTQVATFEQSVQREIARVIVGAEDVIRALCIALIARGHVLVQG
                     PPGLGKTLLAKTLARVLGGEFKRVQGTADLMPSDIVGTHIFDASKNEFVFRRGPLFAD
                     VLLVDEINRTGPKTQSALLEAMEERQVTSERDLYPLPEDFLVLATQNPREFEGTYPLP
                     ESQLDRFMLRIDMSYPEREAEGEILKRYGTELEARDAIPADLGGIDRVLLHQARAAAS
                     ALHVSEALSTYVLDLAHATREHPRVALGLSTRGALALLRAARVHAGLRGGEFVSPDDV
                     QAVARWVLAHRLVLKPEAALDGETDLGIVGAILDATPVPR"
     gene            complement(9626..10987)
                     /locus_tag="VEW08_08175"
     CDS             complement(9626..10987)
                     /locus_tag="VEW08_08175"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HYJ40751.1"
                     /translation="MRERGVAFLLALAALAAFYGLWLRPSPSFDPDANIARPTTAERR
                     GNGYAGLYEWLQRSGVEVRSLRERYGALSDLDLSPRGNLLILSLPAVEVFHSDELSAL
                     DRWVRRGNTLLINAALLDQPDWAARRSAGAVVEIESLTAIEFETPRARETRLDDTPLA
                     QRVREADARAAKHKDGDEDEDSEDEFDAERGKLLEVPDKITLTATGPHVLLEGVKTLE
                     LVTDYSAEDWSLRMPYDNFVLTLARTAGGEGALFEQRVGEGRVLLSAGGSLFTNRALG
                     NADNARLLANIVSARMSPNGVVLFDDLRQGLSASYDPARFYEDARLYKTMFIVLGLWL
                     VWVLGSTRLRAPPVELHDPSEAELVRHAGGLIARTVTPSRTAVRLFELFFARVDRAVR
                     RAGGKAPERGDQWQWLERHGAILPQELDQMKAWYADAHSARKLPIIELQNLLDHLESR
                     LKT"
     gene            complement(10987..11916)
                     /locus_tag="VEW08_08180"
     CDS             complement(10987..11916)
                     /locus_tag="VEW08_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40752.1"
                     /translation="MKRWLGLLGLIAWGVAQPADDAALRAVESCRARLDARVDIGIER
                     IQKRCPDLMPALAGAPWRNLLPSSMRERREEITAESLRALAELVRQSRDGASSRDAPT
                     RDRLEPVLANLDDQGEQAVTRWERFKRWLKDKLENREDEDDAGWLDKFARQFKTSEGV
                     AQFITYTGYALVGVLVALVIWSEVRAAGFFGGVRRISARQNPAAEWRRRLLLADVANA
                     PLAERPGMLLRLLGEALTRAQRLPAAEGLTASAIARRARLDSPDEREALRAVGATAEA
                     VRFAAASPPDADIEGTVVTAKALLENFARQGGR"
     gene            complement(11913..12746)
                     /locus_tag="VEW08_08185"
     CDS             complement(11913..12746)
                     /locus_tag="VEW08_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40753.1"
                     /translation="MYRAQTVDAAGQLPPDRPLMTAEVLDAAYRLFRAGIFRCLPYSG
                     LAVLVLELPTLYSTFLIPRGSGVSFVIPNYRLIGYATVFVMSVVLLGVITLRLNAVAQ
                     GVRPGFRREIAIALKRLPTALLATLFAFAYPAAMLVVVPVLDAALPTEALIFAAVPLL
                     WPVALFVVALPAFWCDGLGPFAAIARAVRLSIHRSWRLVGIILTTALMLMAFNLLPFS
                     VAAMLSPIFGSVDLFLVAMLASIFYLVIGAFGVPFVLAVLIVAYQDLKVRDQERRGAS
                     P"
     gene            12794..13654
                     /gene="prmC"
                     /locus_tag="VEW08_08190"
     CDS             12794..13654
                     /gene="prmC"
                     /locus_tag="VEW08_08190"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HYJ40754.1"
                     /translation="MSDLTGRLDLSDSERLLANAARTIHRRDAEILLAACWRKSRSQL
                     LANIGETVPVEVAASFEDARRQRADGVPVAYLLGRREFWSLDFAVNPAVLVPRPETEL
                     LVQRVLDLVGATEADVADLGTGSGAIAIALAHERPGWRVLGTDISELALAVARGNGER
                     LAGGRVEWASGDWYAALGSRRFDVLVSNPPYVAADDPVLSEDGLRHEPRTALTPGNDE
                     IGDGFASLATLINGAPGHLRPGGWLALEHGATQGETLRALLVARGFTHVTSHRDLAGH
                     ERVTEGKLAP"
     gene            13672..14154
                     /locus_tag="VEW08_08195"
     CDS             13672..14154
                     /locus_tag="VEW08_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYJ40755.1"
                     /translation="MFETTLGNFEVELFEKEAPISSANFLEYVDDGFFDGVIFHRVIP
                     KFMIQGGGFEPGMKQKKVKAPIKNEAANGLKNSRGTLAMARTNDINSATAQFFVNLVD
                     NDFLDHSGPGNYGYAVFGKVTSGMDVIDQIAKVATTTRSGHQNVPAADVIITKASRKV
                     "
     gene            14151..14918
                     /gene="mtgA"
                     /locus_tag="VEW08_08200"
     CDS             14151..14918
                     /gene="mtgA"
                     /locus_tag="VEW08_08200"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HYJ40756.1"
                     /translation="MKKNWRRRLVRWTLIGLAAAFLVTALPVVAMRWMDPWYSAFMLD
                     AALDASRAGKTNYQTDYRWVDLEQISPHAAVAVIAAEDQFFPFHTGFDFKSIREAVRH
                     NESQATKRRPRVRGASTITQQVAKNLFLWSGRSYLRKGLEVYFTLLIEITWPKERTLE
                     VYLNIAQFGEGIYGVEAAAQRFYRMPAARLGRYEAATMAAVLPNPITFRVNAPSNYVV
                     KRRDWILAQMRGLGGARYLDELENPTAPPRAREPAGK"
     gene            15065..16156
                     /locus_tag="VEW08_08205"
     CDS             15065..16156
                     /locus_tag="VEW08_08205"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HYJ40757.1"
                     /translation="MNENPYERASADAPAPSPSLPRVLLPFEFRGNGGEYFRIWIVNL
                     LLTIVTLGIYSAWAKVRRLRYFYGNTYLDGQSFDYHGQPLAILKGRLIVFGGYLVFVF
                     ASNKFPIGSSVAALLLVIFGVPWIIMRARKFQLRMTSYRNLRFNFHGTYGGAMSSFIG
                     WYFLAVITLGILFPVWLRKRVQYSLDNASYGNQQFSFLTGNGTYYKFCFITFGLGILV
                     YGCLFVVMKSVFGLGNVVTDIETMANIGVWVVLFVFLVAMLGIAGYYQASAVNAAFGG
                     VQAGSQFVRSELRAWPLIGIYVSNLFAIVLTLGLFYPWAKVRQVRYQLENMAVDSDGN
                     LAAFTASSGEGIDAVGEEVGDFFDVDFGL"
     gene            16158..17300
                     /locus_tag="VEW08_08210"
     CDS             16158..17300
                     /locus_tag="VEW08_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYJ40758.1"
                     /translation="MIAGRYFDGRSAAGVDASLEFGADGNVHVHGAVVPIAFPLAEVN
                     ISERVGNITRRITFPDGGVFETQDNDAIDEARDALGLKRGLAIVHWLESRWRVAVGSL
                     VAVVLVSVAFIHWGVPAMANWAAKVMPAEVDQSIGAGTLDILDRVAFEPSELPNTRQQ
                     ELRKRFIDMTRPLTDGHEYQLEFRHGGMIGANAFALPSGIIVMTDELVEMSKKDDELV
                     SVLAHEIGHVRGRHALRQMLQAAGVSALAVALLGDVSSISAVLSAAPALLHAKYSRDF
                     EREADSFAKQWLKANGIAESNFDAILCRISDAYEEKRPKDGNEEKDDFDYFASHPSTS
                     ERARCVVEEAEAKPAGEAEVAPEAEPVPEREEAITEEQPVDTTRPD"
     gene            complement(17303..18193)
                     /locus_tag="VEW08_08215"
     CDS             complement(17303..18193)
                     /locus_tag="VEW08_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HYJ40759.1"
                     /translation="MSDLVRRFVLERHPIRGHAVRLSLSWQALREHQDYPPAVQHLLG
                     EAVGAVVLLAATLKFDGVLTLQLQGKGLVSLLVAQCTHDFKVRAMARHDPIGGEAGFR
                     SLAGEGQIVVTVEAADRASSYQGVVPITGNSLAESLEAYFHQSEQLPTRVLLAASSGV
                     VAGMLVQRIPGAGGTQVQLDEAALESAWQKADAAMSALARGQLLEDDVEQRLVEMFGV
                     DEVRVFSGHEVKFECRCSRERVANVLRSLGVEEVRSVIEEQGACTVTCEFCQKPYKFD
                     AIDVEHLFDGTAPRGSDSIN"
     gene            complement(18197..19666)
                     /gene="gatB"
                     /locus_tag="VEW08_08220"
     CDS             complement(18197..19666)
                     /gene="gatB"
                     /locus_tag="VEW08_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HYJ40760.1"
                     /translation="MSAPDGWEIVIGLEIHAQLSTKSKIFSGSPTAYGASPNSQANLV
                     DLAYPGVLPVLNTEAVRMAVKFGLAVDATIAARSVFARKNYFYPDLPKGYQISQYELP
                     VVAKGHMDIVLDDGTKKRIGITRAHLEEDAGKSLHEGLPKLSGIDLNRAGTPLIEIVS
                     EPDMRSAKEAVAYLKKVHTLVRYLEICDGNMQEGSFRCDANVSVRKVGAEKFGTRAEI
                     KNINSFRYVEKAINYEVARQIDVLEGGGKVVQETRLYDPDKGETRSMRSKEEANDYRY
                     FPDPDLLPVEIDAAYIAGVKATLPELPDQKAARFAEQFGLSEYDAGVLTSSKELAAYY
                     EEVAKLLAGGKEPSREQAKLSANWVAGSLAAALNEHNLEIGESRIGAAGLAGLLGRIA
                     DNTISGKIAKDVFEAMWAEGKDADAIIESKGLRQITDTGAIEKAIDEVMAKNPGQLAD
                     YRSGKDKLFGFFVGQVMKATGGKANPAQLNELLKKKLAG"
     gene            complement(19663..>20019)
                     /locus_tag="VEW08_08225"
     CDS             complement(19663..>20019)
                     /locus_tag="VEW08_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HYJ40761.1"
                     /translation="YLKAQRVRQLINQDFKRAFAEVDVLMGPTAPDVAFGIGAKASDP
                     IQMYLNDIYTIGANLAGVPAMSIPCGFVRDLPVGLQIAGPHFAEAKLLNVAHAYQKET
                     DWHRRIPAAFQKEKLS"
BASE COUNT         3542 a         6655 c         6329 g         3493 t
ORIGIN      
        1 gcatctgcag atccttgacg atcgcagcct gcttcttgtc gtcgacaatg agattgatga
       61 tcgaggaata gaacttctcg ttgtccacca gcgtcatcag cttctggtgg ttgagagtga
      121 tggcgcgcgg ctccgcgtga ccaccgtaaa ggattgccgg cactcgtccc gcgtgacgca
      181 ggcggcggct cgcacctttg ccctccgcat cgcggaactc ggcggcgagt tcgaaagaaa
      241 tacgcatgat tgaaactcca gttacatcaa tattcgacgg acaccgcgac cagtgccgcc
      301 acccaagagg tgccgggccg ggaaaccggg ataactcggg gcctgcgcca ctcgctgagt
      361 tatcccggtt tcccggcccg gcaccgtcta atccagatac aggctgctca ctgagtcttc
      421 gtcgcggatg cgacggatgg tctcggccag caaccccgca accgacaact gccggatgcg
      481 tccacattgt ctcgcctcgg tcgagagcgg gatggtatcc gtcaccacca gctcatcgat
      541 cgcggacttc gaaatcttct cgatggcatt gccggagagg atggcgtgcg tgatgtaagc
      601 caccaccttc agggcgccct cgtccttcaa tgcctgcgca ccggcggcca gcgtgcccgc
      661 ggtgtccacc atgtcgtcga tgagcacgca ggtcttgccg cgcacctcac cgatgatgtt
      721 catcaccttc gactcgttcg gccgcggacg gcgcttgtcg aggatggcca ggtccgcatc
      781 gtcgagccgc ttcgccagcg ctcgcgcgcg caccacgcca ccaacgtccg gcgacaccac
      841 gatcaggttc tcgtagcgct gccggtatgc atcgcccagc aacaccggag atgcgtacac
      901 gttgtccacc gggatgtcga aaaatccctg tatctggtcc gagtgcaggt cgatcgtcag
      961 cagacgatcc acacccgcgc tctgaatcat gttcgccacc agcttcgcgg tgatcgccga
     1021 gcgcgtggcg cgcggccggc gatcctggcg cgagtaaccg aagtacggca ccaccgccgt
     1081 gatcttggcc gccgatgcgc gccggcaggc atcgacgatg accagcaact ccatcaggtg
     1141 gtcgttggtc ggcggacacg tgggctgcag gatgaacaca tcccgcccgc gcacgttctc
     1201 catcaactcg atgttcacct cgccgtcgga aaaccgcccg acgtaggcgc ggcccagcgg
     1261 tgtcgtcagg tgacgcgcga tgtcgtgagc cagcgccgga ttggcgttgc ccgcaaacaa
     1321 ggtcagcgtt tctgattcgg ccactagcta ctactcccac tcgataatct tcttgaatgg
     1381 ggacaggttg aaggcaacct gtcccgcatc cgattactgg ctggggtgga aggattcgaa
     1441 cctccgaatg gcgggatcaa aacccgctgc cttaccactt ggcgacaccc caactgccta
     1501 actcttcgag cagcgggcca gttcttccag caaaggagaa ctgtccagcc cgcgcgcgac
     1561 ccacgcgcgc cactcatccg gcactcgtgc tgcgacgcgc tccgcgtctt cgacgcgcga
     1621 gaatttcgcg aacacgcacg atcccgtacc cgtgagtcgc gcagtgccgt gccgcgcaag
     1681 ccaatccaac gcctcagcca ccgccggaaa gcgcgcacgc acgactgctt cgcaatcgtt
     1741 acgcccgccc gtttccaaaa agccgcgcat tgtggtgggg ggtgaattcc gtgtcaattc
     1801 agcagcctgg aatacggcgg ccgtgggaac gctaactccg ggaaatatga cgaggtacca
     1861 ggaatccgcc ccgagactca ccggcgtgag cttttcgccg atgccttcgg cccaggacga
     1921 gcgcccggct gcgaaaacgg gcacatctgc tcctaaagca gcgccaaaag ccccgatctg
     1981 ctccgatgta tagcccaaat tccacatctg attgagcgcc agcagggtcg tagccgcatc
     2041 ggaactgccg ccgcccatcc cgccccccag gggaattctt ttcctgaccg aaatttccgc
     2101 ccccagccgc gtgccactca cctgctggag cgccctggct gcccggacga ccagatcctc
     2161 ggattccggg acgcccgccg ccccgagcgg acgactgatc gaaccgtccg tccggacggt
     2221 gatctcgatg cgatcgcaga ggtcgatgag ctgaaaaacg gtctgcagct cgtggtaccc
     2281 gtcctggcgg cggccgacga cgtgaagcat caggttgagc ttggccggtg cggaccaggc
     2341 caccatttcc gaagcgctca cggctgccag ttttctacca gcagcttcag acgcgcgccc
     2401 tcgcgttcgg ccgtgagccg ctgcggcaac gcaaatccga ggccgggagc acgcgaattg
     2461 atgctgacgt gccagccgtt ctgcgtgaaa tcatggatct caccgctgcc cgcatcctgg
     2521 ttgatgctga cctcgcccgg cgccggaagg cccagcaacc accagcgaag ctcggtcaac
     2581 ggcaactgaa agccgagtcg tcgctcgagt tcatcgcgcg cggcttgtcc atcgagcttc
     2641 tcgccgcgac tggtggcgat ggcaatgccc tcgccctcga tgtccacgcg caggccgccg
     2701 attccgagcg ggccgtcgag cgcgagattc gagctccggg actgctgttg ataacgaagg
     2761 ctggcagaga agccctgccc ctgcgcggcg accgcgacac gtccgctcag gccataacgg
     2821 tcgagctttt cgagagcggc gcgctgctcg ggccacggag cgtcggctcc ggggccaatg
     2881 tccgcactgg gcggcagcgt ccggcagccg gccgacaaca gcagcacggc catggcggcc
     2941 gtggcggcga gagcgccgcg cactgctaac tactcgtgga cgtgggcgga ggctgcaata
     3001 tgggccccgg tccgccgtcc gggtcgggag cgtcgctgtc gggcttcggc ttggcaggcg
     3061 gttcgagctt cgtgccggtc agccgttcga tggtgtcgcg cagcggcttc gagtccggcg
     3121 cacgtgccag cgcccgcgcc cacagcgtgc gcgcttccga ctgtttgccg ttgacccaca
     3181 ggacctcgcc ccagtgcgag gcgatttcgg agtcggggaa gatgcggtag gcgcgctcca
     3241 gcagaggtat tgcgccggaa acgtcaccac gccgaaagag cacccagccc aggctgtcga
     3301 gaaacgcggg attgtcgggg gatgccgtga gtgccttgcg aatcagcgtt tccgcgcgcg
     3361 gcagtttctg gttgcggtct gccagcgaat agccaagcgc gttcaacagg ctggcgtctt
     3421 ccggacgatc tttgagcagg ctttcgaagc tgcgcaccga ttcgcgctcc atgcccgcct
     3481 tgtcctggat gagcgcgatc tggtagcgaa cacccggatg atcgggaaaa cgttcgagcg
     3541 caagctgcag caacgcgatg gcttcgtttg aatggccgac atccgcgagc aacgccgcct
     3601 tggcgaattc cacgtcgaga atttccgagc gttccttggc cgcgtgttcg tcgagcatgc
     3661 ggaacgcggc gtcgcgctcg ttcttggcga gcagcaggcg cgccgcgcgc ccgcggacga
     3721 tgaggccacc gcccgcttcg gcgaggcggg agtaaccctc gagtgcgaga tccgtgcggc
     3781 cttcgcgctc ggcgatggac gacaggaaga agaacgcctc gggcgcctcc gcaggcgagc
     3841 tgatcaaact accgaatcgg cgcccggctt cggtcaggtc gcccatctgg taggcaagct
     3901 tgcccagccg cagatccgct tcaccgcgga tggacgcatc ctcgcgcatg ctctcgagct
     3961 cctggcgagc ttcgtcgatt cgatcgaggc gcatcagggt gtcgacgtag gcgaaacgtt
     4021 cggtgtccgg atcgagagcc gccgcttcct gtgcaacggc gactgccgca acggcgtcgc
     4081 cttcggccgt aagtactctt gcgagctgtg cgcgcgccgt gccggatgcc ggctcgttgg
     4141 agagcatgcg gtcggcaagc ttgtgcgcgg cagcgaaatc gaatgactcg agcgccaggt
     4201 cgacgcccgc ggcgagcagc ctttccgaga cctcgggcga atcgagccgg ggccgcaggg
     4261 ttttcagtgc gatggcggaa ccaccggcat cgctgacttc acccatgagc ttgatgagcg
     4321 actcgtcgcc gcccagggct tgtgtgcgca ggattgccgc tgaagcttca tcgggttggt
     4381 agagccgcac ggcggcgagc gccaccgcgg cggacgcacc cgcgctttcg ggatcgagtt
     4441 tttgccagcg ccgcgcggcc ttcgcgctcg cgctgatctg ctggcattcg gaggcgatct
     4501 cgctggcgcg cttggaaatg ctggcgtcgt tcgaggcgaa tgccgccttg atgtaacggt
     4561 cggtgccacc ccggcagtcg ccgcgcgaca gcgccatgtc ggcgagcacg agatcggcgc
     4621 tgtcggaatt ggtggtggac aacgccatga cgcccgccgg caacagcgcc gccagggcca
     4681 gtgacaggag catcgaagcg gtttttcgtg gcatggaagg cactataact ggacctccgc
     4741 cccttacaat ggttcgcgtg aaagacacca ttcgactgcg gctcgaaaaa atggtcgacc
     4801 gctatgagga aatcgggcgc tcgctggcag accctgcggt catcggccga caacgtgaat
     4861 tccgcgagtt atcgcaggaa tatgcccgcc tgacgccggt ggcggaaaag ttcggcgcgt
     4921 tccagacgct cgagcgtgag ctgaagtcgg cccgcgacat gcagaacgac agtgatccgg
     4981 gcatgcgcga aatgggccag gaagaaatga cccgcctcgc tcccgaaatc gagcgcgccg
     5041 aagacgcgct gaagatgttg ctcatcccgc gcgacccgcg cgacgacaag aacatctatc
     5101 tcgaagtccg tgccggcacc ggcggtgacg aggcggccat cttcgccggc gatctgtttc
     5161 gcatgtacgc ccgcttcgcc gagacacatg gtttcacggt cgagatactt tccgagagcc
     5221 tgggagagca cggcggctat cgcgaggtca tcagccgcat cgcgggcgca ggcgcgttcg
     5281 cgcgcttcaa gttcgagtcc ggcgtgcatc gcgtgcaacg tgtaccggcc accgaagcgc
     5341 agggacgcat tcacacctcg gcctgtaccg tggccattct tcccgagccc gatgaagtgg
     5401 aagcctcgga tctgaatccg gccgagctgc gcatcgacac ctttcgcgcc tcgggcgcgg
     5461 gcggccagca cgtcaacaag accgactccg ccatccgcat cacgcatctg ccctcgggtc
     5521 tggtggtcga gtgccaggac gagcgttcgc aacacaagaa ccgatcacgc gccatggcgc
     5581 tcatgaaagc caagctgatg gccgcggaac aggaaaaacg cgacagcgaa atcgccagga
     5641 accgcaagct gcaggtgggc acgggcgatc gttccgagcg catccgcacg tacaactttc
     5701 cccagggccg cgtcaccgat caccgcatca acctcacgtt gtacaagctc ggcgacatca
     5761 tgcagggaaa aatcgacgaa ctgctggact cgctgcaaca cgagcaccag gcagagcagc
     5821 tcgcggagga agaacagcgg ggcgctgcgt gaacgcggcc gcccgcgggc tcgaggcggc
     5881 tccgatgctg ggcgtcgcga gcgccaccgg tgtcgacgcg catctcgtgc tggccggccc
     5941 cggcgcccgg tctctcgcct tcctgctcga ctggctcatt cgcaccgcac tcgcggcggc
     6001 gtacctgctg ctcgcatcgt ggctgttact cggcaacttt catttcgaaa tcggtcccga
     6061 cgacgaatcg ctgtggtacc tggccggcgc gctgccggcc acgctgatct atcttctcta
     6121 ccacctcatc ctcgagccat tgatggcggg ccgaacgccg ggcaagcgcc ttgccgggct
     6181 gcgcgtattg acactcgaag gcctggtccc atcggcgggc gcgctgatca cgcgcaatgt
     6241 tttccgcctg atcgattcca tgccggtgtt ctatgtcgtc ggactgctgt tcgtgttctt
     6301 cggccggcaa caccagcgcc tcggcgacat agcggccggc accgtgcttg cggttgaacg
     6361 cgcgccgttc ctcgaaaagc tggcgcggtg gcgtcgcgac cccacggcgc acggtgaatg
     6421 gcgggccgcg cgacaacgcg ccgcggcgct cagtcgccgc cgtggcaccg acgtggacga
     6481 cgcgctcgcc gtagtcgacg actatcgccg cgccgcgcac gcgctcgcca tcgagcggca
     6541 atccgcgcct cccgttcgct ccgccgaata cctggaagcg gtgtattccg atctgcacga
     6601 caccattcac cggcccgcac gccgcattcc gctccagctt ctctcgttga tgcgtgatcg
     6661 gatcccggcc gacctgagtg tcatgaaggt gcacatcctg tccgtcacgc tgctgttcat
     6721 cgtgtcggtg gtcacgggcg cgtggctggt ctacagctac ccggacctca tcgcgctgtt
     6781 cgcgaatccg cagctcatcg ccaccgtcga gcgtggcgag ttgtggaccg atggactgct
     6841 caacgtcact ccgtcagcgg tgctctcggt gtccatcctc accaacaaca tcgtggtgtc
     6901 gtttttcgcg ttctgctcgg gcatcgtctt tggcctcggc accttctacc tgatcggcac
     6961 gaatggcctg tcgctcggcg ccatattcgc gttcaccacc cagcacgacc tcggcgggcg
     7021 gctgttcgac ttcatcgttg ctcacggatg cgtcgagctc tcgtgcatat gcatcgccgg
     7081 cgctgccggc tcttacctcg gcgaagcgtt gattcggccg ggagaacgct cgcgcagcga
     7141 agcgtttcgc gccgcggcgg ccgaggggat tcgcgtgatg ctggtggtca cgctgttgct
     7201 gctcatctgc ggcttcatcg agggatacgt gtcgccggac ccggaagtcc cgcgctgggc
     7261 acgcgtcacc atcggcgtag gttactggct gttcatgatt tcgctgttgc gcggctacgt
     7321 gttcggtcgc agccgcggcg gggaaattgc gacctaggga agagcttccc tggcgctcag
     7381 attcgccggc ggctgcgcag cctcaggtaa gaatcgagta cggccttgtc gagtttgttg
     7441 tcggcttcat cgagcacgac tatcccccgc tgccgcaatt gcatcacctg caattgcgcg
     7501 cgctgatgat gttcgcgtgc ggcaatcttg acggcgggat cgcgccacgc ccgtgccgga
     7561 tccgcggtga ggcgctcgat ctcggcggcg tgcggcgcgg cgacgatggg ctgatgacgc
     7621 ggcacgagcg ccttcagcgc ccgcatcagc aggtcattgc gcgccggatc ggcgagatcg
     7681 gtgagccaaa ctaccagccc acggtggcgc aacagccgcc gaacgtgaat cgccgcggcc
     7741 accggattgg attcaccgcg gtcactctcg aggatctcga gctggtggcg caaccgggcg
     7801 acagcgttca gtccacgctc cggaatgctc gcgctcaaag tacgatccgc gtacaccagc
     7861 aatccgacgc ggtcttctat cgagaccgcg tattcggcca gcctcgcggc cacgttcgcg
     7921 tagagcccga gccggtcgag catgccggcg cgcacgcggc tgctgcggcc ggcatcgatc
     7981 accagcagga tctccagatg ctgggcttcg ctgtattcgc gtgaaatcag cgtgccgcgg
     8041 cgcgccgtgg ctttccagtc gatacgcgac agtgcatcac caccgacgta gtcgcgcagc
     8101 tgcagcagtt ccacgcccgt gcctggcagc cgtcgcggcg tttcaccggc ggattcaccg
     8161 ggcaccggcc gccccctgtt cggcaggctg tccggcgcca ctgtgaacgc cgtgtcctgc
     8221 tgcagttcgc gcgcccacca cgcgagagcg aaccgcccta acaaccgtgc gggcacgttg
     8281 ccgaaccggc cggcgccgag tcgcaacggc agcagctcgg ccggatcgcg cagctctccc
     8341 tcgggcggca gcgttatgca ccgcacctcg gtggtctggc gcagcgccgg cggcaacacg
     8401 cgggcgtact gcaacacgat ctcgcggccc cgattgtgcg caaaggcgta cgcaccccgc
     8461 acctgcctgc caagtttcag gcggtcgtcg taacgcatgg ccatcgtcac cttcgtaccg
     8521 cgcaggtacc acgcctcgat cgccaggccc agcagcagca ggaatgccgg gagcaaccag
     8581 gcccccgcga atgccggcac gtccgaccac gtcccggcca cacctaacaa ggcggtaagg
     8641 gcgacgaaca ggtagccgcg cagcgcgaag tgcattggtc agcgcggtac cggggtggcg
     8701 tccaggatcg cgccaacgat gccgaggtcc gtctcaccgt cgagcgccgc ctccggcttg
     8761 agcaccagcc gatgcgcgag cacccagcgg gccaccgcct gcacatcgtc cggactcacg
     8821 aattcacccc cgcgcaagcc cgcgtggaca cgcgccgccc ggagcagcgc cagcgcgccg
     8881 cgcgtcgaca gaccgagcgc cacgcgcgga tgttcacgcg tggcgtgcgc caggtcgagc
     8941 acgtaagtgg agagcgcttc cgacacgtgc agcgcactgg cagcggcgcg cgcctggtgc
     9001 aggaggacgc gatcgatgcc ccccagatcg gccggaatgg catcgcgcgc ctcgagctcc
     9061 gtgccataac gcttcaggat ctcgccttcg gcctcgcgct ccggataact catgtcgatg
     9121 cgcagcatga agcgatcgag ctgggattcc ggtagtggat aagtcccttc gaactcgcgc
     9181 gggttctgcg tcgccagcac caggaagtct tccggcagcg gatatagatc gcgctccgaa
     9241 gtaacctggc gctcttccat ggcttcgagc agcgccgact gcgttttggg tccggtgcgg
     9301 ttgatctcgt ccaccagcaa cacgtcggcg aacagcggcc cacgccggaa cacgaattcg
     9361 ttcttcgacg catcgaagat gtgtgtaccg acgatgtcgc tcggcatcag gtccgcggtg
     9421 ccttgcacgc gcttgaactc gccacccagc acgcgtgcga gcgtcttggc caacaacgtc
     9481 ttgccgagac ccggcgggcc ctgcaccagc acgtgaccgc gggcaatgag cgcgatacac
     9541 agcgcgcgga tgacgtcctc ggcgccgacg atgactcgcg cgatctcgcg ctgcacggat
     9601 tgttcgaagg ttgcgacttg ggtgttcatg ttttcaatcg tgattccagg tgatccagga
     9661 ggttctgcaa ttcaatgatc ggcagcttgc gggcggagtg cgcatccgca taccaggctt
     9721 tcatctggtc gagttcctgc ggaaggatgg cgccatggcg ctcgagccat tgccactggt
     9781 cgccacgttc gggcgccttg ccgcccgcgc ggcgcacggc acgatccacg cgcgcgaaga
     9841 acagctcgaa caggcgcacg gcggtgcgcg acggtgtgac ggtgcgggcg atgagaccgc
     9901 cggcgtgccg cacgagctcg gcttccgacg gatcgtgcag ctcgacggga ggagcccgca
     9961 gccgtgtgga acccagcacc cagaccagcc acaggccgag cacgatgaac atggtcttgt
    10021 acaagcgcgc atcttcgtag aaccgcgcgg gatcgtaact ggccgaaagc ccctggcgca
    10081 ggtcatcgaa caacaccacc ccgttcggtg acatgcgcgc gctgacgatg ttggccagga
    10141 ggcgcgcatt gtcggcattg ccgagcgcac ggttggtgaa cagtgaacca ccggcgctca
    10201 gcagcacgcg cccttcaccc acgcgctgtt cgaatagcgc accttctccg ccggcggtgc
    10261 gcgccagcgt gagcacgaag ttgtcgtacg gcattcgcag cgaccagtct tcggccgagt
    10321 aatccgtcac gagctcgagg gttttcacgc cttcgagcag tacatgcggg cccgttgcgg
    10381 tcagcgtgat cttgtccggg acctcgagta gtttgccgcg ctccgcgtcg aattcgtctt
    10441 cggaatcctc atcctcgtcg ccatccttgt gtttggcggc gcgcgcatcc gcctcgcgca
    10501 cgcgttgcgc cagcggagtg tcatcgagcc gggtctcgcg cgccctggga gtctcgaact
    10561 cgatcgcggt cagcgattcg atttccacca ccgcccccgc cgaacgccgc gcggcccagt
    10621 ccggctgatc gagcagcgcg gcattgatga gcagcgtgtt accgcgccgg acccatcggt
    10681 cgagcgcact gagctcatcg ctgtgaaaca cctccaccgc gggcagcgag aggatcagca
    10741 ggttgccacg cggcgacagg tcgaggtcag acagcgcgcc gtaacgttcg cgcagcgacc
    10801 gcacctccac gccggaacgc tgcagccatt cgtacaggcc ggcgtagcca ttgccgcgac
    10861 gttctgcggt ggtgggccgc gcgatattgg catcaggatc gaacgacggc gacgggcgca
    10921 gccacaggcc ataaaacgcg gcgagggccg ccagcgccag cagaaatgcg acgccgcgtt
    10981 cgcgcatcag cgaccgccct gccgggcgaa attttcgagc agcgctttcg ccgtgaccac
    11041 ggttccttcg atatccgcat caggaggcga agcggcggca aatcgcactg cttcggcggt
    11101 agcgcccacg gcgcgcagcg cctcgcgttc gtcgggcgaa tcgaggcgcg cacgcctcgc
    11161 aatcgcggaa gcagtgaggc cctccgcggc cggcaggcgc tgcgcgcgcg tcagcgcctc
    11221 gcctaacaac cgcagcagca ttcccgggcg ctcggccagc ggcgcattcg ccacgtccgc
    11281 gagcagcagc cgccggcgcc attcggccgc cgggttctgg cgcgccgaga tgcgccgaac
    11341 accgccaaag aacccggccg cgcgcacctc cgaccagatg acgagagcca cgagtacgcc
    11401 gaccagcgca taacccgtgt aggtgatgaa ttgcgcgacg ccctcgctgg tcttgaactg
    11461 ccgggcgaac ttgtcgagcc agcccgcgtc gtcctcgtcc tcgcggtttt cgagcttgtc
    11521 cttgagccag cgtttgaagc gctcccaccg cgtcacagcc tgctcgccct ggtcgtcgag
    11581 attggcgagc accggctcca ggcggtcacg cgtgggagca tcccggctcg aggcgccgtc
    11641 gcgcgactgc cgcacgagct cggcgagcgc ccgcaggctt tcggcggtaa tttcttcgcg
    11701 ccgctcgcgc atcgacgacg gcagcagatt gcgccatggc gcgccggcca gcgccggcat
    11761 caggtccggg caacgtttct ggatacgctc gatgccgata tcgacgcggg catcgagtcg
    11821 cgcgcggcag gattccaccg cgcgcagcgc cgcgtcgtcg gccggttgtg cgacacccca
    11881 ggcgatgagc cctaacaacc cgagccagcg tttcacggcg aggccccgcg ccgttcctgg
    11941 tcgcgcactt tcagatcctg gtaagcgacg atgagcaccg cgagcacgaa gggcacgccg
    12001 aaggcgccga tgacgagata gaaaatggag gcgagcatcg cgaccaggaa aagatcgacg
    12061 ctgccgaaga tcggcgacaa catggctgcg acgctgaacg ggagcagatt gaacgccatg
    12121 agcatgagtg ccgtcgtcag gatgatcccg accaggcgcc acgagcggtg aatcgacagg
    12181 cgcaccgcgc gcgcaatcgc cgcgaacggc ccgaggccat cacaccagaa tgcaggcagg
    12241 gccaccacga acagcgccac cggccacagc aggggcaccg cggcaaatat cagcgcttcg
    12301 gttggcaggg cagcgtccag caccggcacc acgacgagca tcgccgccgg ataggcgaac
    12361 gcgaacagcg tggcgagcaa ggcggtcggc agccgcttga gcgcgatggc aatttcgcgg
    12421 cgaaatcctg gcctcactcc ctgggccacc gcgttgagcc gcaatgtgat cacgccgagc
    12481 agcacgacgc tcatcacgaa caccgtggcg taaccaatca gtcggtaatt cggaatcacg
    12541 aacgagacgc cactcccgcg aggaatcagg aacgtggagt acagggtcgg caattcgagc
    12601 accaggacgg caagcccgga atacggcagg cagcgaaaga tgccggcgcg aaacaggcga
    12661 tacgccgcgt ccagcacctc ggccgtcatg agcggccggt ccggcgggag ttgcccggcc
    12721 gcatccactg tttgcgctcg gtacatgccc ggcatgatac cgatgcgccg gggaagtcgt
    12781 gcatcatcgc tgcatgtccg acctcaccgg caggctcgac ctgtccgaca gcgaacgtct
    12841 cctggcaaac gccgcacgca cgatccatcg ccgcgatgcg gaaatcctgc tcgccgcctg
    12901 ctggcgcaag agccgctcac aactgttggc caacatcggc gaaacagtgc ctgtcgaggt
    12961 cgcggccagt ttcgaggatg cgcgccggca gcgcgccgac ggcgtacccg ttgcctatct
    13021 tctgggccgg cgcgaattct ggtcgctgga cttcgcggta aatcccgcag tgctggtgcc
    13081 gcgccccgaa accgaactgt tggtgcagcg agttctcgat ctcgtcggcg cgacagaagc
    13141 tgacgtcgcg gacctgggca ccggttcagg cgcgatcgcc attgcgctcg cccatgagcg
    13201 cccgggttgg cgagtactcg gcaccgacat ctccgaactg gcgctggcgg tggcccgggg
    13261 aaacggcgaa cgcctcgccg gtggacgggt cgagtgggct tcgggcgact ggtacgccgc
    13321 gctgggcagc cgccgtttcg acgtcctggt cagcaatccg ccctacgtgg ccgcggatga
    13381 ccccgtgctc agcgaagacg gcctccgcca cgaaccccgg accgcgctga cccccggcaa
    13441 cgatgaaatc ggcgatggat tcgcgtccct ggccactctt attaatgggg cccccgggca
    13501 ccttcggccc ggcggctggc tggcgctcga acatggcgcc acacagggag aaacgcttcg
    13561 tgcgctgctt gtggcccgtg gcttcactca cgtaacctcg caccgcgatc tcgccggcca
    13621 cgagcgcgtc accgaaggca agctggcccc ataggaaacc ccgtgtcact agtgtttgaa
    13681 accacgctcg gcaatttcga agtcgagctg ttcgagaaag aagccccgat ctcttccgcg
    13741 aattttctgg agtacgtcga cgacggcttc ttcgatggcg tcatcttcca tcgcgtcatc
    13801 ccgaaattca tgatccaggg cggcggcttc gagcccggaa tgaagcagaa gaaggtcaag
    13861 gcgcccatca agaacgaggc ggccaacggc ctgaagaatt cgcgcggcac gctcgccatg
    13921 gcgcgcacca atgacatcaa cagcgccacc gcgcagtttt tcgtcaacct ggtggacaac
    13981 gatttcctcg accactccgg cccgggcaac tacggctacg ccgtcttcgg caaggtcacc
    14041 tccggcatgg atgtcatcga tcagatcgcc aaggtggcaa ccaccacccg ctccggccac
    14101 cagaacgtgc cggccgcgga cgtgatcatc accaaggctt cacgaaaggt gtgaagaaga
    14161 actggcggcg gcggctggtc cgatggacgc tcatcgggct cgccgccgcc tttctcgtta
    14221 cggcgctgcc cgtggtggcg atgcgctgga tggacccgtg gtacagcgcg ttcatgctcg
    14281 acgcggcgct cgacgccagc cgggctggga agactaacta ccagaccgac taccgctggg
    14341 tggatctcga acagatctcg ccgcacgccg ccgtggcggt gatcgccgcc gaggaccagt
    14401 tctttccgtt ccataccggc ttcgatttca agtcgatccg cgaggcggtg aggcacaacg
    14461 agtcccaggc gaccaagcgc cggccgcgcg tgcgcggcgc cagcaccatc acgcagcagg
    14521 tggcgaagaa cctgttcctg tggtccggaa ggagctatct acgcaagggc ctcgaggtgt
    14581 acttcacgct gctcatcgag atcacctggc ccaaggaacg caccctcgag gtgtatctca
    14641 acatcgcgca gttcggtgaa ggcatctacg gcgtggaagc tgccgcgcag cgcttctacc
    14701 gcatgccggc cgcccggctc ggccgatatg aagccgcgac catggcggcg gtgctgccga
    14761 acccgatcac gttcagggtg aatgcgccgt ctaactatgt ggtgaagcgt cgcgactgga
    14821 tcctggcgca gatgcgcggg ctaggtggcg cgcgttatct cgatgaactc gagaacccca
    14881 ccgccccgcc gcgggcacgt gagccggccg ggaaatgagc gtgccacgag tccgggaatc
    14941 gtgacaccac agtcgcgata tcgctcccgg cagggctggc cgccgattgc tctggcgtcc
    15001 gccctctcac ccggattacg ctagccgcag gttcgccgac ggcgacccca ctgggagaac
    15061 aataatgaat gagaacccgt acgagcgggc gagcgcggac gcgcctgccc cgtcaccgag
    15121 cctgccccgc gtcctgttgc cattcgagtt ccgcggcaac ggcggcgaat atttccgcat
    15181 ctggatcgtc aacctgctgc tgaccatcgt cacgctggga atctattccg cgtgggcgaa
    15241 ggtgcgccgg ttgcgctatt tctacggcaa cacctatctc gacgggcagt cgttcgacta
    15301 tcacggacag ccactcgcca tcctgaaggg ccggctcatc gtgttcggcg gatacctggt
    15361 gttcgttttc gcgtcgaaca agttcccgat cggctccagc gtggccgcgc tgctgctcgt
    15421 gatcttcgga gtgccgtgga tcatcatgcg ggcacgcaaa ttccagctgc gcatgacttc
    15481 ttaccgcaac ctgcggttca actttcatgg cacgtacggc ggcgccatgt cgtcattcat
    15541 tggctggtat tttctcgccg tcatcacgct cggcatactg tttccggtct ggctgcgcaa
    15601 acgcgtgcag tattcgctgg acaatgcctc gtacggaaac cagcagttct cgttcctcac
    15661 gggcaacggc acctactaca agttctgttt catcactttc ggtctcggca tcctggtcta
    15721 tggctgcctc ttcgtagtca tgaaatcggt gttcggcctt ggcaacgtgg tcacggacat
    15781 cgagaccatg gccaatatcg gcgtctgggt agtgttgttc gttttcctgg tcgccatgct
    15841 cggcatcgcc ggctactacc aggcgtcggc cgtcaacgcg gcgttcggcg gcgtccaggc
    15901 cggctcgcaa ttcgtgcgtt cggagctgcg cgcgtggccg ctcatcggca tctacgtgtc
    15961 gaacctgttc gcgattgtac ttacgctcgg gctcttctac ccatgggcca aggtgcgaca
    16021 ggtgcgttac cagctcgaaa acatggcggt cgattcggac ggaaatctcg cggcattcac
    16081 cgccagctcc ggcgagggaa tcgacgcggt cggcgaggag gtcggcgact tcttcgacgt
    16141 ggacttcggt ctctagcttg atcgcgggtc gatacttcga cgggcggagc gcggctggcg
    16201 tcgatgccag cctggagttc ggcgcagacg gcaacgtgca cgtgcacggg gccgtcgtgc
    16261 cgatcgcgtt ccccctcgcc gaggtcaata tctccgagcg cgtcggcaac atcacgcgcc
    16321 gaatcacctt tccggacggc ggtgtgttcg agacgcagga caacgatgcc atcgatgagg
    16381 cgcgcgacgc actcgggctg aagcgcggcc tggcgattgt ccactggctc gaaagccgct
    16441 ggcgcgtcgc ggtcggctcg ctcgtggcgg tcgtactggt gtccgtggcg ttcatacact
    16501 ggggcgtgcc cgccatggcg aactgggcgg cgaaggtgat gcccgcggag gtcgaccagt
    16561 cgatcggcgc cggcactctg gacatactcg accgcgttgc tttcgaaccc tcggagctac
    16621 ccaacacacg tcagcaggaa ctgcgcaaac gcttcatcga catgaccagg cctctgaccg
    16681 atggccacga gtaccagctc gagttccggc acggcggaat gatcggagcg aatgcattcg
    16741 cgctgccctc gggcatcatt gtcatgaccg acgaactggt ggagatgtcg aagaaggacg
    16801 atgagctggt ctcggtactg gcccacgaaa ttggtcacgt gcgtggtcgc catgcactgc
    16861 ggcagatgct gcaggcggcg ggcgtctctg cgctcgccgt cgccttgtta ggcgacgtga
    16921 gctccatctc cgcggtgttg agcgcggccc cggcgctgtt gcacgccaag tactcgcgtg
    16981 acttcgaacg cgaggccgac tctttcgcca agcagtggct gaaagccaac ggaatcgccg
    17041 aatccaattt cgacgccatc ctgtgccgca tcagcgacgc gtacgaggaa aagcgcccga
    17101 aagacggcaa cgaagagaaa gacgatttcg actacttcgc ttcgcatcct tcgacctccg
    17161 agcgggcgcg atgcgtcgtg gaagaagctg aagccaaacc cgcaggcgag gcggaagtag
    17221 cgccggaagc ggaacccgta cccgaaagag aagaagccat cacagaagaa cagcccgtcg
    17281 ataccacgcg cccggactga gctcagttga tcgagtcgct tccgcgcggc gctgttccgt
    17341 cgaacaggtg ctcgacgtcg atcgcatcga acttgtaagg cttctggcag aactcgcagg
    17401 tgactgtgca ggcgccctgc tcttcgatca ccgaacgcac ttcttccacg cccagcgagc
    17461 gaagcacgtt cgccacgcgc tcacgcgagc agcggcattc gaatttcacc tcgtggcccg
    17521 agaacacgcg tacttcatcg acgccgaaca tctcgaccag gcgttgctcc acgtcatctt
    17581 ccagcaactg gccacgcgcg agcgcggaca tggcggcatc ggccttttgc caggcggatt
    17641 cgagcgctgc ctcatcgagc tgaacctgcg tgccgccagc gccgggaatg cgctgcacca
    17701 gcatgccggc gacgacgccg gaactcgcgg ccagcaatac gcgcgtgggc aattgttcgg
    17761 actggtggaa ataggcttcg agcgattcgg ccagtgaatt gccggtgatc ggcaccacac
    17821 cctggtaact ggacgcgcgg tcggcggctt cgaccgtcac cacgatctgt ccttcgcccg
    17881 cgagcgagcg gaaacccgcc tctcccccaa tcggatcgtg ccgtgccatc gcacgaacct
    17941 tgaagtcgtg ggtgcactgc gcgaccagca ggctgacgag ccccttgccc tggagttgca
    18001 acgtgagcac gccgtcgaac ttcagcgtgg ccgcgagcag caccaccgct ccgacggctt
    18061 cgcccagcaa atgctggacg gcgggcggat agtcctggtg ttcgcgcaat gcctgccagg
    18121 acagggaaag gcgcacggcg tggccgcgga tcggatgccg ctcgagtacg aaacgccgga
    18181 cgagatcgga cacgactcag ccggctaact tctttttcag aagctcgttg agctgggcag
    18241 gattggcctt gccgccggtg gccttcatga cctggcccac gaaaaagccg aacagcttgt
    18301 ccttgcccga gcggtaatcc gcgagctgcc ccggattctt cgccatgact tcgtcgatgg
    18361 ccttctcgat ggcgcccgta tcggtgatct gcctgagacc cttcgattcg atgatggcgt
    18421 cggcgtcttt gccttccgcc cacatggcct cgaacacatc cttggcgatc ttgccggaga
    18481 tggtgttgtc ggcaatgcgg ccgagcaggc ccgcgagccc cgcggcaccg atacggctct
    18541 cgccgatctc gagattgtgt tcgttgagcg cggcggcgag cgatcctgcc acccagttgg
    18601 cggacaactt cgcctgctcc ctggagggct ccttgcctcc ggcgagtagt ttggcgactt
    18661 cctcgtagta tgccgcgagt tctttcgacg acgtcagcac gccggcgtcg tactccgaca
    18721 ggccgaactg ctcggcaaac cgcgcggcct tctggtccgg cagctcgggc agcgtcgcct
    18781 tcacgccggc gatgtacgcg gcgtcgatct ccaccggcag caggtccgga tcggggaagt
    18841 agcgatagtc gttcgcttct tccttcgagc gcatgctgcg cgtctcgccc ttgtcagggt
    18901 cgtagagccg cgtttcctgc accaccttgc cgccgccttc gagcacgtcg atctggcgcg
    18961 caacctcgta gttgatggcc ttctcgacgt agcggaacga gttgatgttc ttgatctcgg
    19021 cccgcgtgcc gaacttttcg gcgccaactt tgcgcaccga cacgttcgca tcgcagcgga
    19081 acgagccttc ctgcatgttg ccgtcgcaga tctcgaggta acgcaccagc gtgtgcacct
    19141 tcttgagata ggccacggcc tctttcgccg agcgcatgtc gggctcggag acgatctcga
    19201 tgagcggcgt gccggcacgg ttgagatcga tgccggagag cttcggcagg ccttcatgca
    19261 gcgacttgcc cgcgtcttct tcgagatgcg cgcgcgtgat gccgatgcgc ttcttcgtgc
    19321 cgtcgtcgag cacgatgtcc atgtgaccct tggcgaccac gggcagctcg tactggctga
    19381 tctggtagcc cttgggcaga tccggataga agtagttctt gcgcgcgaac accgaccttg
    19441 cggcgatggt cgcatcgacg gccagcccga acttcacggc catgcgcacc gcttccgtgt
    19501 tgagcacggg caatacgccg ggatacgcga gatcgacgag gttcgcctgc gagttgggcg
    19561 aggcgccata tgccgtgggc gagccggaga agatcttcga cttcgtcgac agctgcgcgt
    19621 ggatctcgag accaatgacg atttcccaac catcgggagc gctcatgaca gcttctcctt
    19681 ctggaatgcg gcaggaatgc ggcgatgcca gtccgtttcc ttctggtacg cgtgcgccac
    19741 gttgagcagc ttcgcttcgg cgaaatgcgg gccggcgatc tgcagaccca cgggcagatc
    19801 gcgcacgaat ccgcagggga tggacatggc gggaactccc gccaggttcg cgccaatcgt
    19861 gtagatgtcg ttcagataca tctggatcgg atccgaagcc ttcgcgccga tgccgaaggc
    19921 aacgtcggga gcggtgggac ccatgagcac gtcgacttcg gcgaacgcgc gcttgaagtc
    19981 ctggttgatc agctgacgca cgcgctgcgc cttcaggta
//
LOCUS       DASQJS010000200        15658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_111457, whole genome shotgun sequence.
ACCESSION   DASQJS010000200 DASQJS010000000
VERSION     DASQJS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15658
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111457"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="VEW08_08230"
     CDS             <1..1452
                     /locus_tag="VEW08_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HYJ40762.1"
                     /translation="PDYIRALGRAYELEQSPAARDAIAQILTNSRMCAEGRRPICQDT
                     GIVVVFAKVGMNVRWDASMSFEDMINEGVRRAYLHPDNKLRASIVADPNFSRRNTKDN
                     TPAVIHTQLVAGEELEITVAAKGGGSENKSKFVMLNPSDSLAEWVLKTVPLMGAGWCP
                     PGMLGIGIGGSAEKAMLLAKEALMEPIDMAELKARGPASKTEELRIELYDKVNALGIG
                     AQGLGGLSTVLDVKIFEYPTHAASKPVAMIPNCAATRHVHFTLDGSGPAVLDPPRLSD
                     WPKIDWAPAADARRVNLDALTPAEVASWKPGEQLLLNGKMLTGRDAAHKRIADLFASG
                     KELPAGVDFRNRVIYYVGPVDPVRGEVVGPAGPTTATRMDKFTEMMLEKTGLIAMVGK
                     AERGPVGIEAIRKHRAAYLMAVGGAAYLVSKAIKASRVVAFEDLGMEAIYEFTVVDMP
                     VTVAVDANGTSVHQTGPAEWSQKIAAGISVRGR"
     gene            complement(1680..3893)
                     /locus_tag="VEW08_08235"
     CDS             complement(1680..3893)
                     /locus_tag="VEW08_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYJ40763.1"
                     /translation="MVSPTPAEARTTPPLTDGSDARLRALTEHALEIITVQSADGTFT
                     YSNEAVARYLGYAVSELLGHNATEFLHPDDAEAMRERFRSLLSNRGSQPELNRFEYRF
                     RHRDGSWIWLESVAVNALDNPTVLGIIAHSRDINRRKANERILSLNHARYRTVADLSE
                     GAVHEYLMNSQGVYELEWTLGAERAYGCTDEEYRRRGWQSFLEGDEWREQSQARTELY
                     VRGETVEFTAQIRRPDGELRWLEVRNRPIADPATGKFTRLVGVAIDITERKHAADALR
                     ESEFRYRTVAELTSGFVYESTVDERGEAHVVWASPGWNEFFGGSFEELNRLGWRHVFF
                     PVDHDAAIQRRQRLRSGERTEMELALKTLRGEVRWIHLANQPVFDTDGKVTRFIGVVH
                     DITDRKFSEEVLRSQALAIEVMHEGVVLSDSGGIIRMTNPAFDRMFRIVAGSFIGKKL
                     NQLPCDPPLDRRIAESADEVAARAGAPLVSELTVHGATDGDTALFVEAAITSLVLRGE
                     RFWLTMLQDATARRQLEREVLEVSNREQQRIGNDLHDGLGQELTGIALLLRGLESRAE
                     RESPTLSPSIEEVALLVNDAIFTTRALARGLSPVTFDRGGLALALEELTRRLSTMFHI
                     DVRCVADAALDHGLESVNALHLYRIAQEAVTNAAQHGHAEQVQISLSVDGGRGLLRIE
                     DNGNGFNPAIHESKGLGLRIMHYRAQMMAGSLRIDSARSRGTIVSCWFPRGEGSG"
     gene            complement(3932..4291)
                     /locus_tag="VEW08_08240"
     CDS             complement(3932..4291)
                     /locus_tag="VEW08_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40764.1"
                     /translation="MQLSRVLRIAACLAGLTAISAVGFAVPTLPAQPPLRVGSECQAD
                     KPEPKSLAFVVRYVERRPAGEFILTFENGEIWQQDDRNKKVIIERGEQVVIRRSTTGV
                     FTLVSRDGQTTRVKRMH"
     gene            complement(4325..6028)
                     /locus_tag="VEW08_08245"
     CDS             complement(4325..6028)
                     /locus_tag="VEW08_08245"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HYJ40765.1"
                     /translation="MNATANDIRRMVEAGRTADAEVALRRKLAEVPTNAEWRTLLARV
                     LAATGRKPEAIAELGIAVGHAPGGREARLTLARLANELGQSSLAAQHARVLTERDARD
                     SEAWSALGMAAFGLRNKREATRALRRAVDIAPAYGAARYNLAAVLADQERSEEALDEA
                     AAALRHGAKPRSVSLVQTRALIQLDRFEDAERQLLALLRGDAGDVEAHGMLLQLRQLR
                     GDEEPLAALRAAAQAPTASPRLRLAFANAARRSGATAEAEASLRELMKAFGPDPMLLS
                     SLATVLQESARTGEALDAARAGIAHLPDDAVVAENFVVAALCAGATPEALPFVEKFRA
                     LKPRDQRWITYRLDIARLRGEEEFASWFDPARVVSIVDLPPPTGFATTGEFHSALLPV
                     LQARHRQQNHPLDQSLRHGTQTSRNLLVNPGAEIATLLQSFRQALADMQSRSGRDPNH
                     PFEARNLSPAEILGCWSVRLRRGGFHVNHIHPEGWVSSAYYASVPGEVDDATQRSGWL
                     KFGEPRFAVPGLAPFLEVQPKPGRLVLFPSYLWHGTNALRDDAPRLSVAFDAVPQAEL
                     G"
     gene            6595..9474
                     /locus_tag="VEW08_08250"
     CDS             6595..9474
                     /locus_tag="VEW08_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ40766.1"
                     /translation="MSNSSITNSVRFALATAATASLSQLSMQTAMAQEQDQDATESME
                     EVIVTGSRVRRLDTESASPIVTIDKASIEKSGVTTAGDLLQRLPTVSGAATNPAVNNG
                     GGFGEANVELRGLDAKRTLVLLNGRRLGLVGASDATDINLIPVNLIERVDVLKEGAGA
                     IYGSDAIAGVVNFITRKDVEGAELTAGYGETAENDGKTQNVNLLWGTSGDNFRFSING
                     NYNKTEKVSAGDRAFSEFAMYFYSGSVFPGGSSRVPTGRFYVPAGSGLPPNVANCASV
                     TRVAGSDGTDLATDYRCYNGQTDAYNYQPFNLIMTPQERGSIFANYEYDINEAVQLYA
                     EVLYSRTSSGFQIAPLPFDALADDVVISADSYYNPFGYDFGGATTGNPNLTVRLEALG
                     NRRSKTSSDSKVAKIGARGGLFGSEWNYDFLVGYSRLDQAASVSGYLYQPGLAGAFGP
                     SFDAGGGVIQCGTPTNPIPLGQCTPVNVFNLADPAQVSALGTISSGYDTDRIYTSKMA
                     SFAVDGKMFSMPAGDALASIQADYTELRGDFNVDFLITATPPLYLNCFLAQETCTGPN
                     SGGYDVSELSAEVFLPLVKDVTGVQALNLTLGTRYSEYSIFDSTTNSTVKLEYRPVED
                     ILLSGTFAEVFRAPTILDLYASPANNSSTFTDPCVGLTTARITANPNLALACVGVPTD
                     GSFEQPNGQVTGLLMANEDLDPETGDVMTFGITWQPSFLNNNFSIRVDYWEYQIDDVI
                     TQLDTNFSINQCVATGDPAFCNLVTRFTDPQNAGIVYLFRQPTVNLGTLETDGVDIGA
                     SYHLRDTPAGNWAFQIDWTHINKYNNLPAENAAIVEVAGTYDRQYGNYAENRATATIG
                     WSMNKFDAQIGARFIDDIVLLLPSGGTLPPEDNPPLPIDSFVYLDLYFGYTFNDNIKV
                     SLSATNLSDEQPPMLFQNNVTNANTDVNTYDTLGRRYAISATYKF"
     gene            9554..11404
                     /locus_tag="VEW08_08255"
     CDS             9554..11404
                     /locus_tag="VEW08_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HYJ40767.1"
                     /translation="MTDSAPSTATVEHAGRLLAEGKHAQAEAEARAVLARSPGDPGAR
                     VVLNASLLFAQRYTEAIPVAESLIESAPREPAHWMNLGTARRGARQYDAALEAYMRAA
                     ALGASGSDFSFNVALTHIERGDLDAARSLLAGARDQDPFDGEICFQYAQVCYETLRID
                     EASAALAEWHTLQRLDTTLIANIGLLLTNLGETRRAEVALRQARADKAAPTAALLTVI
                     AVLERTNRLAEARELMDRLLADPKSDLQTPERMALEGQLLQREGKHSEAEALFTRALP
                     GKDEFHRRHQRLFPIARSLDALGRYDDAWRVLVEAHASQVDSIQRQSPQWALRAMPDM
                     SVTARGCDAADVAAWVNDGPSAAESPVFVVGFPRSGTTLLELALDAHPQLKSMDEQRF
                     VQDALDELMAAGADYPQRLRDVSREVLDRVRGRYWERAAKKVKLAPGERLVDKNPLNI
                     LRLPAIRRVFPNSRILLAVRHPCDVILSCYMQHFRAPDFALLCRSIESLALGFRRTFD
                     YWYAQNAMLAAAVHELKYEDFVRDFESGTRAVTDFLELPWAESMLRPAERAQAKGYIS
                     TPSYAQVTQPVNSKSVDRWRRYEKHFSAAMPPLEPYLARWNYAGAAANSR"
     gene            complement(11374..11964)
                     /locus_tag="VEW08_08260"
     CDS             complement(11374..11964)
                     /locus_tag="VEW08_08260"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HYJ40768.1"
                     /translation="MFATPHQDLRHYLRVYDDALPATWCTRLLKGFDALAAHQARNGR
                     ELRAGLEDSAWTELNVTRHGDPLTAGFFRGRIDEALARYNADVQLDIPIPNSQVFADL
                     VLKRYRPNSAERFQLHFDAINHLANRYVVLLWYLNDVERGGATRFPQLGLDVAARAGR
                     LLMFPPYWMYQHEGLPPESGDKYILSTYLLFAAAPA"
     gene            11991..13265
                     /locus_tag="VEW08_08265"
     CDS             11991..13265
                     /locus_tag="VEW08_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HYJ40769.1"
                     /translation="MKAAEQGHLLLKQGQAKEAELVFRRVLEEEPDHVEALNIVGLAA
                     LRDGDIDRALTLLKRAAAVEGGQALTHLHLGRAHAAAFEFDAAAAAYATALERAPALH
                     AARLHLAELHEKRGDTMRAVMTYARALNDAQGEGRWLNAATTPAGLQGAVSHAHQFVR
                     ERRLAGFAELMEPFTRQYGGGALRRVDQAVRIHLHMEQPVFPDPRQKPSFLFFPGLPA
                     TPYLDKGLIPEIEDLEAATPDIRAELLALLPSAAGRERVFHTGELEEANLRGLEEPPS
                     WNGYYFFRHGERRADNCAACPKTAAALDALPLSRVPGHGPEVLYSVFSPGTHLLPHCG
                     VTNTRVVGHLPLLIPEDCALRVGGEDHHWREGEVVVFDDTYDHEAWNRSGRTRVVMIF
                     DLWNPFLTQAERAVLAELVVAMGEFRAGIEAL"
     gene            13269..15077
                     /locus_tag="VEW08_08270"
     CDS             13269..15077
                     /locus_tag="VEW08_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HYJ40770.1"
                     /translation="MRLPLPFIRLPVRFDLARLRAEVASLPEEVWAPHPNHIAGNTSL
                     RLVSVEGGENDDVDGVMRPTPHLAKLPYVRQVLASFGVVWSRSRLLRLDPGAVVPEHA
                     DINYHWFYRVRVHIPIVTRPEVLFHCGGETVHMRGGEAWLFDNWRLHRVENPTPEARI
                     HLVADTSGSASFWQFVAQAAQPGMVDRELRFDPARDGVLLTEQSPLRPVMSPGEVDYL
                     VGDLRSELVPSVDAPESRARLARYHQLLDGFVRDWRQVYSLFGERADARGQFEQLRDT
                     ARTASRELAEGLMMRTNRVAAHPVFEGRVLRSVLPQKPGTASVKIPRIERPVFIIAAP
                     RSGSTLLFETLAESHSICTLGGEAHGAIEGAQPSLRPGAPGVDSNRLERQHATPEVIR
                     RLHEEFAAQRRPAPGAHGADVAGLRFLEKTPKNALRIPFLEAAFPDAQFIFLWREPRA
                     NLASIIEAWKAGRWVTYRGLDELPDPWSLLLPPGWREMRDKPLPEIAAFQWETANRIA
                     LDDLSKIDRSRWTAVTYEELVANPRAVVERLTKFIGIGIDAGLEQRLAAPLPPSRQTN
                     TLPDTQKWRLHEAVIEPLLSNLTPIVTRLRALSGPT"
     gene            complement(15081..15413)
                     /locus_tag="VEW08_08275"
     CDS             complement(15081..15413)
                     /locus_tag="VEW08_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40771.1"
                     /translation="MKTFNFTLFAASFVLLGACAGTPEKPAPTAEDVPPPANVTLDEI
                     NLAIENGVQLQNEKGEEMVCRREPKVGSRLARETICMTKTEWLRVADESQRATQKSMR
                     TGKIPHGK"
     gene            complement(15410..>15658)
                     /locus_tag="VEW08_08280"
     CDS             complement(15410..>15658)
                     /locus_tag="VEW08_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40772.1"
                     /translation="NGAGRATFVRMEHLRDEMLKMLTSTGLVISGPMREWIAAAPARN
                     VSAHGRYVEYYGTELRDLVAERDAELIAAHGYRFGELN"
BASE COUNT         2757 a         5010 c         5074 g         2817 t
ORIGIN      
        1 ccggactaca tccgcgcgtt gggccgtgcg tacgagctcg agcagtcgcc ggcagcgcgc
       61 gacgcgatcg cgcagatcct caccaactcg cgcatgtgcg ccgaaggccg ccggcccatc
      121 tgccaggaca ccggcatcgt cgtggtgttc gccaaggtcg gcatgaacgt tcgctgggat
      181 gcctcgatga gcttcgagga catgatcaac gaaggcgtgc gccgcgccta tctacatccg
      241 gacaacaagc tgcgcgcgtc gatcgtcgcc gaccccaact tctcgcgcag gaatacgaaa
      301 gacaacacgc ccgccgtcat ccacacacag ctggtggccg gcgaagagct cgagatcacg
      361 gtggcggcga agggcggcgg ctcggaaaac aagtcgaagt tcgtcatgct gaatccctcg
      421 gattcgcttg cggaatgggt gctcaagaca gtgccgctca tgggcgcggg ctggtgtccg
      481 ccgggcatgc tgggcatcgg catcggcggc tcggctgaaa aggccatgct gctcgccaaa
      541 gaggcgctca tggagcccat cgacatggcg gagctcaagg cgcgcggtcc tgccagcaag
      601 accgaagagc tgcgcatcga gctgtacgac aaggtgaatg cgctcggcat cggcgcgcag
      661 ggcctcggtg gactatctac cgtgctcgac gtgaagatct tcgagtaccc gactcacgcg
      721 gcgtccaaac ccgtggccat gatcccgaac tgcgcggcga cgcgccacgt gcatttcacg
      781 ctcgacggca gcgggccggc ggtgctcgat ccgcccagac tctccgactg gccaaagatc
      841 gactgggcgc cggccgccga tgcgcggcgc gtgaatctcg acgcactcac gcctgcggaa
      901 gtggcgagct ggaagccggg cgagcagctg ctgctcaacg gcaagatgct caccggccgc
      961 gatgccgcgc acaaacgcat cgccgacttg ttcgccagcg gcaaggagct tccggccggc
     1021 gtcgattttc gcaatcgcgt catctactac gtcgggccgg tcgatccggt gcgtggcgaa
     1081 gtggtgggtc ccgcgggtcc gacgaccgcg acgcgcatgg ataaattcac cgagatgatg
     1141 ctcgagaaga ccggcctcat cgccatggtg ggaaaggccg agcgtgggcc ggtcggcatc
     1201 gaggcgatcc gcaaacatcg cgcggcctat ctcatggccg tgggtggcgc cgcctacctg
     1261 gtgtcgaagg ccatcaaagc ctcgcgcgtg gtggcgttcg aagatctcgg catggaagcc
     1321 atctatgagt tcacggttgt ggacatgccg gtcaccgtcg ccgtcgatgc gaacggaacg
     1381 tcggttcacc agaccggacc cgcggagtgg agccagaaga tcgccgccgg aatttctgtt
     1441 cgcggccggt aagcgctggt caggcaacca tgcccggacc ccgcattttc acgatcgcgt
     1501 tgcggagagc aggaccggaa gaagtaggaa gtcgaccaga tcatcgagtg gctgacgggc
     1561 tactcgccgg cggcgctgaa caaacacatc atgagtgagg tcgatttcga gacctttttc
     1621 gcgtgtgcgc cgtcgatcaa ccccggtgtt tcgctcatca agggcgaggt ttgcggggct
     1681 caaccgctac cttcaccccg cggaaaccag caactgacga tggttccgcg gctgcgcgcg
     1741 ctgtcgatgc gcaggctgcc ggccatcatc tgcgcgcggt aatgcatgat gcgcagcccg
     1801 agccccttgg actcatgaat cgccggattg aagccgttgc cgttgtcctc gatgcgcagc
     1861 aggccgcggc cgccatcgac gctcagcgat atctgcactt gttcggcatg tccatgttgt
     1921 gcggcgttgg tcacggcttc ctgcgcaatc cggtagaggt gcagcgcatt cacggattcg
     1981 agaccgtgat ccagcgcggc gtccgcaacg caacgcacgt cgatgtggaa catcgtcgag
     2041 aggcggcgcg tgagttcttc gagtgccagc gcgagtccgc cgcgatcgaa agttactggt
     2101 gacaggccgc gcgccagtgc gcgcgtcgtg aagatggcgt cgttgaccag cagcgcgacc
     2161 tcttcgatcg aaggcgacag tgtcggtgat tcgcgctcgg cccggctctc gagcccgcgc
     2221 aacagcagcg cgatgccggt gagctcctga ccgaggccgt cgtgcagatc gttaccgatg
     2281 cgttgctgct cgcggttgct gacctcgagc acttcgcgtt cgagctggcg ccttgcggtg
     2341 gcgtcctgga gcatggtgag ccagaaacgt tcgccacgaa gaaccagcga agtgatggcc
     2401 gcttcgacga acagcgcggt gtcgccgtcg gtggcgccgt gaaccgtgag ttcgctcacg
     2461 agcggcgcgc cggcgcgggc cgcgacttcg tcggctgatt cggcgatgcg ccggtcgagg
     2521 ggcgggtcgc agggaagctg attcagcttc ttgccgatga atgatccggc gacgatgcga
     2581 aacatgcgat cgaacgccgg gttggtcatg cggatgatgc cgccgctgtc ggagagcacc
     2641 acgccttcat gcatcacctc gatggccagt gcctggctgc ggagcacttc ctcgctgaac
     2701 tttcgatcgg tgatgtcgtg gaccacgccg atgaatcggg tcaccttgcc gtcggtatca
     2761 aagaccggct gattggcgag gtgaatccag cgaacctcgc cacgcagcgt tttcagtgcc
     2821 agctccatct cggtgcgttc gccgcttcgc agccgctgcc gacgctggat ggcggcatcg
     2881 tgatcgacgg gaaagaaaac gtggcgccaa ccgaggcggt tgagttcttc gaacgaaccg
     2941 ccgaaaaact cattccagcc cgggctggcc cagaccacgt gcgcttcgcc gcgttcatcg
     3001 acggtggatt cgtagacgaa accggacgtc agttccgcga ccgtgcggta gcggaattcg
     3061 ctttcgcgca acgcgtcggc ggcatgtttg cgctcggtga tgtcgatcgc gacgccgacc
     3121 aggcgcgtga actttcccgt ggctggatcg gcgatcggcc gattcctcac ttcgagccag
     3181 cggagctcgc cgtcgggccg ccggatctgc gcggtgaact cgacggtctc gccgcgcacg
     3241 taaagttcgg tgcgggcctg gctttgctcc cgccattcgt cgccttcgag aaagctctgc
     3301 cagccgcggc ggcggtattc ctcgtcggtg cacccgtagg cgcgttcggc gcccagcgtc
     3361 cattcgagtt cgtacacgcc ctgcgagttc atgaggtatt cgtgcaccgc gccttcggag
     3421 aggtccgcca ccgttcgata ccgtgcatgg ttcagcgaaa ggatgcgttc attggccttg
     3481 cggcgattga tgtcgcggga atgcgcgatg atcccgagca ccgtcggatt gtccagagca
     3541 ttgaccgcca cgctttcgag ccagatccag ctgccatcgc gatggcgaaa gcgatattcg
     3601 aaacggttga gctcgggctg ggagccgcga ttggaaagca ggctgcgaaa gcgttcgcgc
     3661 atggcctccg catcatccgg atggaggaac tcggtggcat tgtggcctaa caactcgctg
     3721 accgcgtatc cgaggtagcg cgccaccgct tcgttggaat acgtgaaggt tccgtcggcg
     3781 ctctgcacgg tgatgatttc gagcgcgtgt tcggtgagcg cccgcagccg cgcgtcggac
     3841 ccgtcggtga gtgggggcgt ggtccgggct tctgctggtg ttggcgagac catgtgcgag
     3901 gtcgattcta tcgacacctc tgccctaata gtcagtgcat gcgtttcacg cgggtagtct
     3961 ggccgtctcg cgacacgagc gtgaatacgc cagtcgtgga tcgacgaatc acgacctgtt
     4021 cgccgcgctc gatgattacc ttcttgttgc ggtcgtcctg ctgccagatc tcgccgtttt
     4081 caaacgtcag gatgaattcg ccggcgggac gtcgttcgac gtaacgcacc acgaacgcca
     4141 ggcttttggg ctccggcttg tctgcctggc attcggagcc gactcgcaag ggcggctgcg
     4201 caggcaaagt tggcacggcg aaacccaccg cggaaattgc ggtgaggcct gcgaggcacg
     4261 ctgcaattct gagcacgcgt gaaagttgca tggcgcaaat atagcgttac gccggccgcg
     4321 cggcctagcc cagttcggcc tgtgggacgg cgtcgaaggc caccgagagt cgcggtgcat
     4381 cgtcgcgcaa cgcattcgtc ccgtgccaca ggtaagaggg aaacagcacc agtcggccgg
     4441 gcttgggctg cacctccagg aacggcgcca gtcccggcac cgcaaaacgc ggctcgccaa
     4501 acttgagcca gccgctgcgc tgggtcgcgt cgtccacttc gccgggaacg ctggcgtagt
     4561 acgccgagct cacccagccc tcaggatgga tgtggtttac gtgaaacccg ccacggcgta
     4621 gtcgcaccga ccagcagccg aggatttcgg caggtgaaag atttcgagcc tcgaatggat
     4681 ggttggggtc gcggccggaa cgtgactgca tgtccgcgag cgcttgccgg aacgactgca
     4741 gcagggtcgc gatctccgcg cctggattca ccagcaggtt gcgcgaggtc tgggtcccgt
     4801 ggcgcagact ctggtcgagc ggatgatttt gctggcggtg ccgcgcctgc agaaccggca
     4861 gcagggctga atgaaattcc ccggtggtcg caaaaccggt gggtgggggc aggtcgacga
     4921 tcgagacgac tcgcgcagga tcgaaccagc tcgcgaactc ctcctcgccc cgaagtcgcg
     4981 cgatgtcgag ccggtaggtt atccagcgct gatcacgggg cttcagagcg cggaacttct
     5041 cgacgaaggg cagcgcctcc ggggtcgcgc cggcgcacag ggccgcgacc acgaaattct
     5101 cggcaacgac ggcatcgtcc ggaaggtgcg cgatgccggc gcgcgcggca tccagcgcct
     5161 cgcccgtgcg cgcgctttcc tgcaacaccg ttgccagcga gctcagcagc attggatcag
     5221 gcccgaaggc cttcatgagc tcgcgcaggc tggcttctgc ttccgccgtg gcgccggagc
     5281 gtcgcgccgc gttcgcgaac gcgagccgca agcgcggtga cgctgtcggt gcctgggcgg
     5341 cagcgcgcag agcggcgagc ggctcttcgt cgccacggag ctggcgcaac tgaaggagca
     5401 tgccgtgcgc ttcgacgtca ccggcatctc cgcgcaataa cgcgagcagc tggcgttccg
     5461 cgtcttcgaa gcggtcgagt tgtatcagcg cccgcgtctg gacgagcgaa acgctgcgcg
     5521 gcttcgcacc gtgcctgagc gcggcggcgg cctcatccag cgcttcctcg gatcgttcct
     5581 ggtcggcgag cacggcggca aggttgtaac gcgccgctcc gtaagccggc gcgatatcca
     5641 cggcgcgtcg taaagcgcgg gtcgcttcgc gtttgttccg cagcccgaat gcggccatgc
     5701 cgagcgcgga ccaggcttcg ctgtcgcgcg catcgcgctc ggtcagcacg cgcgcatgtt
     5761 gggccgcgag gctggattgc ccgagctcgt tggcgagacg tgccagcgtg agtcgcgcct
     5821 cgcggccgcc gggcgcgtgc ccgaccgcaa tgccaagctc cgcgatggct tcgggcttgc
     5881 ggccggtggc ggccagcacg cgtgcgagca gcgtgcgcca ctcggcgttc gtcggcacct
     5941 cggcgagctt gcggcgtaac gcgacttcgg catcggcggt acggccggcc tccaccattc
     6001 gccggatgtc gttcgccgtc gcgttcatgt tggaagggta aaggcaaacg cccgagcgcg
     6061 cgacgtggag gtgtctcgcg gattagttag atcgcgtcac ctgcctcccg ggacggcgcc
     6121 aggtcggttt gcggagctga tacccccgtg cccgaatatc gtccggccgt ccgacatatt
     6181 gtctgccgcg ccggatgcgg gcatcctcgc cagcctggcc tggagcgagc cgcatggggt
     6241 gcccatcccg ccccaacagg gccccccaaa cgggctccgt tcgtcgcaga ttcagggcaa
     6301 ttcgccgcat cccgacatcg gcccggggca gctggcacgg gcatgccgga cagttctcac
     6361 agattgctgt actgagggag ggcacgtggc cctgacgcaa cagctagtaa cacagagctt
     6421 ggaaatgcct tgaactctgc ttatgatcgc ggccatgtgt gagtagcgtc ggcgcaaatg
     6481 cttgggcatt tggagtcacg aaaacacgcc ttctacatag aaatacacgg tcggcgaagg
     6541 ggttgggcaa gcgcagcgtt gcacagtcga tcgataccca ttcaggagtt aaagatgtcg
     6601 aattcctcaa tcacgaattc agtgcgtttt gcactggcta cggcggctac agcatcgctg
     6661 tcgcaactga gcatgcagac cgcgatggcg caggagcagg accaggacgc caccgaatcg
     6721 atggaagaag tcattgtcac cggctcacga gtccggcgac tggacaccga gtcggccagt
     6781 ccgatcgtga ccatcgacaa ggcctccatc gaaaagtcgg gcgttactac ggcaggcgac
     6841 ctgcttcaga ggttaccgac ggtttccggc gcagccacca acccggcagt gaacaacggc
     6901 ggcggcttcg gtgaagccaa cgtcgaactg cgcggcctcg acgccaagcg cacgctggtg
     6961 ctcttgaacg gtcgccggct cggtctcgtc ggcgcaagcg acgccaccga catcaacctc
     7021 atccccgtga acctcattga gcgcgttgac gtgctgaagg aaggcgcggg cgcgatctac
     7081 ggctccgacg ccatcgcggg cgtggtgaac ttcatcacgc gcaaggatgt ggaaggcgcc
     7141 gagctcaccg cgggctacgg tgaaacggcc gagaacgacg gcaagaccca gaacgtgaac
     7201 ctgctttggg gcacgtcggg tgacaacttc cgcttcagca tcaacggcaa ttacaacaag
     7261 accgaaaagg tttcggctgg tgatcgcgcc ttctcggaat ttgcgatgta cttctattcg
     7321 ggatcggtct tcccgggcgg ttcgagccgc gtgccgacgg gccgcttcta cgtccccgcc
     7381 ggttccggcc tgccaccgaa tgtcgcgaac tgcgctagcg tgacgcgcgt cgcgggcagt
     7441 gacggcacgg acctcgccac cgactaccgc tgctacaacg gccagacgga tgcgtacaac
     7501 tatcagccgt tcaacctgat catgacgccg caagagcgcg gctcgatctt cgcgaactac
     7561 gaatacgaca tcaacgaagc cgtgcagctc tacgcggaag tgctctacag ccgcaccagc
     7621 tccggtttcc agatcgcgcc gttgccgttc gacgcattgg ccgacgacgt cgtgatctcc
     7681 gccgacagct actacaaccc gttcggctac gatttcggtg gcgctaccac cggcaatccg
     7741 aacctcaccg tgcgcctcga ggcgctcggc aatcgccgca gcaagacatc ctccgattcg
     7801 aaggtggcca agatcggcgc gcgtggtggg ctgttcggtt cggaatggaa ctacgacttc
     7861 ctggttggtt atagccgcct cgaccaggcc gcctcggtct ccggttatct gtatcagccc
     7921 ggccttgcgg gcgcgttcgg cccgtcgttc gatgccggcg gcggcgtgat tcagtgcggt
     7981 acgccgacca acccgattcc gctgggacag tgcaccccag tcaatgtctt caacctggcg
     8041 gatcctgcac aggtctccgc gctcggcacg atcagctcgg gctacgacac ggaccgtatc
     8101 tatacctcga agatggcctc gttcgcggtg gacggaaaga tgttcagcat gccggccggc
     8161 gacgcgctcg cgtcgatcca ggcggactac accgaactgc gtggcgactt caatgtcgac
     8221 ttcctcatca cggccacgcc gcccctgtac ctgaactgct tcctcgcgca ggaaacctgc
     8281 acgggaccga acagcggcgg atacgacgtg agcgaactct cggccgaagt cttcctgccc
     8341 ttggtcaagg acgtgacggg tgtgcaggcg ttgaacctga cgctgggtac gcgctactcc
     8401 gagtactcga ttttcgacag cacgacgaac tcgaccgtca agctggaata ccggccggtc
     8461 gaagacatcc tgttgagcgg taccttcgcg gaagtgttcc gtgcaccgac catcctggac
     8521 ctgtacgcct cgccggcgaa caattcctcg acgttcacgg atccttgcgt cggtctgact
     8581 acggcccgca tcaccgccaa tccgaatctg gcattggcct gcgtgggcgt gccgaccgac
     8641 ggcagcttcg agcagcccaa tggtcaggtc acgggcctgc tgatggccaa cgaagatctg
     8701 gacccggaga ccggcgacgt catgacgttc ggcatcacct ggcagccgag cttcctgaac
     8761 aacaacttct cgatccgtgt tgattactgg gagtaccaga tcgacgacgt gatcacgcag
     8821 ctggacacca acttcagcat caatcagtgc gtggccacgg gcgatccggc cttctgcaat
     8881 ctggtgacgc gtttcactga cccgcaaaac gccggcatcg tctacctgtt caggcagccg
     8941 accgtgaatc tcggtacgtt ggagacggat ggcgtcgaca tcggtgcgtc ctatcatctg
     9001 cgggacacgc ctgccggtaa ctgggccttc cagatcgact ggacccacat caacaagtac
     9061 aacaatctgc cggcagagaa cgcggcaatt gtcgaagtgg cgggcactta cgaccgccag
     9121 tacggcaact acgccgagaa ccgtgcaacg gccaccatcg gctggagcat gaacaagttc
     9181 gatgcgcaga tcggggcgcg cttcatcgac gatatcgtgc tgctgctgcc gagcggcgga
     9241 acgctgccgc cggaagacaa tcccccgttg ccgatcgact cgttcgtgta cctcgatctg
     9301 tacttcgggt acacgttcaa cgacaacatc aaggtgtcgc tgtccgcgac gaacctgagc
     9361 gacgagcagc cgccaatgct gtttcagaac aacgtcacca acgcgaacac ggacgtgaat
     9421 acgtacgaca cgttgggccg caggtacgcc atctcggcga cctacaagtt ctgattctgc
     9481 tgagaggttg attggcctct gaagggccgc gtggcgtttg ccacgcggcc cttctcttcg
     9541 tagggtatcg cccgtgaccg attccgctcc ttccaccgca accgttgaac atgcgggtcg
     9601 cctgttggcc gagggcaaac acgcccaggc cgaagccgaa gcccgtgcgg tgctcgcgcg
     9661 gtcccccggc gatcccgggg cgcgcgtagt gctcaacgcc agcctgctct ttgcccagcg
     9721 ctataccgag gctattccgg tggccgagtc gctcatcgag agcgccccgc gggagccggc
     9781 ccactggatg aatctcggca ccgcacggcg tggtgccagg cagtacgacg cggcgctcga
     9841 ggcgtatatg cgtgccgcgg cacttggagc gtctggatcg gacttctcat tcaacgtcgc
     9901 actcactcac atcgagcgcg gggatctgga cgccgcgcgc tcactgctcg cgggtgcacg
     9961 cgatcaggat cctttcgacg gtgagatctg ctttcaatat gcgcaggtct gctacgagac
    10021 tttgcgcatc gatgaggcca gcgcggcgct tgccgagtgg cacacgctgc agcggctgga
    10081 caccacgctg atcgccaata ttggactact gctcacgaac ctgggtgaga cgcgccgcgc
    10141 agaggtggcg ctgcgacagg cgcgcgccga caaggccgcg cccaccgccg cattgctgac
    10201 tgtcatcgcg gtgctcgagc gcaccaatcg gctcgcggaa gcgcgcgagc tcatggatcg
    10261 tctgctcgcg gacccgaagt cggaccttca gaccccggag cgcatggcgc tcgaaggcca
    10321 gctcctgcaa cgcgagggca agcactccga agctgaagcg ctgttcacgc gcgcgttgcc
    10381 tggaaaagac gagttccatc gccgccacca gcgcttgttt cccatcgcgc gctcgctcga
    10441 tgcgctcggt cgatatgacg acgcctggcg ggtgctcgtg gaggcgcatg cctcgcaggt
    10501 cgacagcatc cagcgccagt cgccgcaatg ggcattgcgt gccatgcctg acatgtcggt
    10561 cacggcgcgg ggttgtgacg ccgcggatgt cgcggcgtgg gtcaatgatg ggccttccgc
    10621 cgcggagagc ccggtgttcg tggtcggctt tcctcgttcg ggcaccacgc tgctggagct
    10681 cgcgctcgac gcgcatccac agctcaagtc gatggacgaa caacgcttcg tgcaggacgc
    10741 gctcgacgag ctgatggcgg cgggcgctga ctatccgcag cggctgcgcg atgtgtcgcg
    10801 cgaagtcctg gaccgcgtgc gcggcaggta ttgggagcgc gccgcgaaga aagtgaagct
    10861 cgcacccggc gaacggctgg tcgacaagaa cccgctgaac atcctgaggc tgccggccat
    10921 ccggcgcgtg tttcccaact cgcgcatcct gctcgcggtg cgccatccct gcgacgtgat
    10981 cctgtcgtgt tacatgcagc atttccgcgc gcccgatttt gcgctgctgt gtcgaagcat
    11041 cgagtcgctg gcgctcggct tccgccgcac gttcgactac tggtacgccc agaacgcaat
    11101 gctcgcggcc gccgtgcacg agctcaagta cgaggatttc gtgcgcgact tcgagtcggg
    11161 cacacgtgcc gtcaccgatt tcctcgagct tccctgggct gaaagcatgc tgaggccggc
    11221 ggagcgtgca caggccaagg gctacatcag tacgccgagt tatgcccagg tgacgcagcc
    11281 cgtgaacagc aaatccgtcg atcgctggcg gcgctacgag aaacatttct ccgcggcgat
    11341 gccaccgctc gagccatatc tcgcgcgatg gaattacgcc ggagccgcgg cgaacagcag
    11401 gtaggtcgag agaatgtact tgtcgcccga ttccggtggc aggccttcat gctggtacat
    11461 ccagtagggc ggaaacatga gcaggcgtcc ggcgcgtgcc gcgacgtcca gcccaagctg
    11521 cggaaagcgt gtggcgccgc cgcgttcgac atcgttgaga taccagagca acaccacgta
    11581 ccggttcgcc agatgattga tggcatcgaa atgcagctga aagcgctctg cggagttggg
    11641 tcgataacgt ttcaggacga gatcggcgaa aacctgcgaa ttgggtatgg ggatgtcgag
    11701 ttgcacgtcg gcgttgtagc gcgcgagcgc ttcgtcgatg cggccacgga aaaatccggc
    11761 ggtcagcgga tcgccatggc gggtgacgtt gagctcggtc cacgccgagt cctcgagacc
    11821 cgcgcgaagc tcgcggccat ttcgggcctg gtgcgcggcc agcgcatcga agcccttgag
    11881 cagtcgcgtg caccaggtcg cgggcaacgc gtcgtcgtag acgcgaaggt aatgccgaag
    11941 gtcttgatgc ggggtcgcga acatcacgcg tatcctagct tcgaacactt atgaaagccg
    12001 ctgaacaagg ccatttactt ctcaagcaag gccaggccaa agaggccgag ctggtattcc
    12061 gccgcgtgct cgaggaagaa ccggaccacg tcgaggcact caacatcgtg ggactcgccg
    12121 cgctgcgcga cggggacatc gatcgcgcgc tgacgctgct caaacgagcc gcggcggtcg
    12181 aaggtggcca ggccctgact catctgcacc tgggacgtgc ccacgcagct gcgttcgagt
    12241 tcgatgccgc cgcagcggcg tacgccacgg cgctcgagcg cgcgcctgcg ctgcatgccg
    12301 cgcgcctgca tctcgcggag ctgcacgaaa agcgcggtga cacgatgcgc gcggtcatga
    12361 cttacgcgcg tgcgctcaat gacgcccagg gtgaaggtcg ctggctcaac gcggcgacca
    12421 cacctgcagg cctgcagggc gcggtgagcc acgcgcatca gttcgtgcgg gagcggcgcc
    12481 ttgccggatt cgccgagctc atggagccgt tcacgcggca gtacggcggc ggagccctgc
    12541 ggcgcgtcga tcaggcggtg cgcatccatc tgcacatgga gcagccggtg tttccggatc
    12601 cacgccagaa gccgagcttt ttgttcttcc cgggacttcc ggcgacaccg tatctcgaca
    12661 agggcctgat ccccgagatc gaagacctcg aggcggcaac gcccgacatt cgcgccgagc
    12721 tgctggcgct gttgccgagc gccgcgggcc gcgagcgcgt gtttcatacc ggcgagctcg
    12781 aagaggcgaa tctgcgcggc ctcgaggagc ctccgagctg gaacggctac tacttctttc
    12841 gccacggcga gcgacgggca gacaactgcg cggcgtgtcc gaaaacggcc gcggcgctcg
    12901 atgcgctgcc gctctcgcgc gtgcctggcc acggccccga ggtgttgtat tcggttttct
    12961 ctcccggcac gcacctgctg ccgcactgtg gcgtgacgaa tacgcgcgtc gtggggcacc
    13021 tgccgctctt gatccccgag gactgcgcgt tgcgcgtggg cggcgaggac catcattggc
    13081 gcgagggcga ggtcgtggtg ttcgacgaca cttacgatca cgaagcctgg aaccgcagcg
    13141 gtcgcacgcg ggtcgtgatg atcttcgacc tctggaaccc gttcctcacg caggctgaac
    13201 gcgccgtgct cgccgagctc gtagtcgcga tgggcgagtt ccgcgccggc atcgaggcgc
    13261 tctagcaaat gcgcctgcca ctgcctttca tacgactgcc ggtgcgtttc gatctcgcgc
    13321 ggctgcgcgc ggaagtcgcg tcgctgcccg aggaggtctg ggcgccgcat cccaatcaca
    13381 ttgccggcaa tacctcgttg cggctcgtca gcgtcgaagg tggcgagaac gacgacgtgg
    13441 acggcgtcat gcggccgacg ccgcatctcg ccaaactacc gtacgtacgc caggttctcg
    13501 cgagcttcgg cgtggtgtgg agccgctccc gtctgctgcg tctcgatccc ggagccgtgg
    13561 tgcccgagca cgccgacatc aactatcact ggttctatcg cgtgcgcgtg cacattccca
    13621 tcgtcacacg ccccgaagtg ctgtttcatt gcggcgggga gaccgtgcac atgcgcggtg
    13681 gcgaggcctg gttgttcgat aactggcgcc tgcaccgcgt cgagaacccg acaccggagg
    13741 cgcgtatcca tctcgtcgcg gatacttccg gcagcgcgag cttctggcag ttcgtggcgc
    13801 aggccgcaca gccgggcatg gtcgaccgcg aactccgttt cgatcctgcg cgcgacggcg
    13861 tgctgttgac cgagcaaagt ccgttacgcc ccgtgatgtc gccgggcgaa gtggactacc
    13921 tggtcggcga cttgagatcg gagctcgtgc catcggtcga cgcgcccgag tcgcgcgcgc
    13981 gcctcgcgcg ttaccaccag ttgctcgatg gcttcgtgcg cgactggcgc caggtgtact
    14041 cgctgttcgg tgagcgcgcc gatgcacgcg ggcaattcga gcaactgcgt gacaccgcgc
    14101 gcaccgcgtc gcgcgagctt gccgaagggc tcatgatgcg taccaatcgc gttgccgcgc
    14161 atcccgtgtt cgagggcagg gtgttgcgct cggtgctgcc gcagaaaccg ggcaccgcgt
    14221 ccgtcaaaat tccgcgcatc gaacgccccg tgttcatcat cgcggcgcca cgctccggca
    14281 gcacgctgtt gttcgagacg ctggcggaga gccattccat ctgcacgctc ggcggggagg
    14341 cgcatggcgc catcgaaggc gcgcaacctt cgctgcgtcc tggcgcgccg ggagtcgact
    14401 ccaatcggct cgagcgccag cacgcgacgc ccgaagtgat tcgtcgcctg cacgaggaat
    14461 tcgcggcgca gcgccgtccc gcgcccggcg cccacggagc cgacgtggcg ggattgcggt
    14521 ttctcgagaa gacgccgaag aacgcattgc gcattccgtt cctcgaagcc gctttccccg
    14581 acgcgcaatt catctttctg tggcgcgagc cgcgcgccaa tctcgcaagc atcatcgagg
    14641 catggaaggc cggcagatgg gtgacctatc gcggtctcga tgagctcccg gacccctggt
    14701 cattgctgct gccgccgggc tggcgcgaga tgcgcgacaa accactgcct gagatcgcgg
    14761 catttcagtg ggagactgcg aatcgcatcg cgctcgatga cctttcgaag attgaccgca
    14821 gccgatggac ggcggtgacc tacgaagagc tggttgcgaa tccgcgcgcc gtcgtcgaac
    14881 ggctgacgaa gttcatcggg atcggcatcg acgccgggct cgaacagcgg ctggctgcgc
    14941 cacttccgcc ttcccgtcag accaacacgt tgcccgacac gcaaaaatgg cggttgcacg
    15001 aggcggtgat cgagccgctg ctatcgaatc tcaccccaat cgtgacgcgt ctgagggctc
    15061 tcagcggccc gacctgagat tcactttccg tgcgggattt ttcccgtcct catcgatttc
    15121 tgggtggcgc gctgagattc gtcggccact cgcaaccatt cggttttggt catgcagatg
    15181 gtttcgcggg caagccgcga gccgaccttg ggttcgcgcc ggcagaccat ctcttcaccc
    15241 ttttcgttct gaagctgtac gccgttttcg atggcgagat tgatctcgtc gagcgtgacg
    15301 ttcgcgggtg gcgggacgtc ctcggcggtc ggcgcaggtt tctcgggggt gccggcgcag
    15361 gcgcccagca acacgaaact cgcggcgaag agggtgaaat tgaacgtttt caattgagtt
    15421 ctccgaatcg gtaaccgtgc gcggcgatca gctcggcgtc gcgctccgcc acgagatcgc
    15481 gtaattctgt tccgtaatac tcgacgtatc ggccgtgcgc ggacacgttt cttgccggtg
    15541 ccgcggcgat ccattctcgc ataggaccgc tgatcaccag ccccgtgctt gtcagcattt
    15601 tgagcatttc gtcgcgcaaa tgttccatcc tgacgaaagt cgctcgcccc gcgccgtt
//
LOCUS       DASQJS010000201         3237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1364613, whole genome shotgun sequence.
ACCESSION   DASQJS010000201 DASQJS010000000
VERSION     DASQJS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364613"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            535..1833
                     /locus_tag="VEW08_08285"
     CDS             535..1833
                     /locus_tag="VEW08_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HYJ40773.1"
                     /translation="MRKIQALKMMFACMLASLWMSTALAQLAPAQADSSLSGGNRWVA
                     TWGAAPINPGLTTIDALFLNDHSRSFENQTVRNIAHVSVGGRRVRVRVSNAFGVLPLR
                     LGAAHVALSRGGASINVTTGRRLTFGGQTSVLIPAGAVMVSDAVDMYVASASDLAVSL
                     YLPGVTEPATFNEFKMHTSYVTGVNSGNLVSAADLPTAEAVRQVFYTTVVEVQPTESV
                     GALVTIGDSITLGGNSTPDAYRTWPDLLSERINTHRPRLSVVNQGIGCNRLLYDLCGP
                     SGAARFDRDVLAVTGVSHVIVAFGINDLMIPSILPQFGFPEFASETVSAQDIIVGLQQ
                     LIVRSRSRGIRVYGATITPNGSITVPGAHTPDTEAKRTAVNRWIRTSGAFDGVVDFDA
                     AVRDPSNPARLLPAYDADGVHLTDAGHQAMANAVNLSMLF"
     gene            1906..>3237
                     /locus_tag="VEW08_08290"
     CDS             1906..>3237
                     /locus_tag="VEW08_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HYJ40774.1"
                     /translation="MRRASLPLFIERGTRTLQEQIYRGIRQSILDGLVSADRRLPSTR
                     SLAADLGVSRTTALLALEQLRAEGYLIARPGSGTFISPRLPGPPAMPVNAPGKPIARP
                     PFSRRGQLLARMQAPDRRANVPPRAFRLGTPALDLFPWRLWAQITRECLRDCAATHLD
                     YSPLAGLRALREAIAEQMQARGTRCDPDQVQVVAGAQRGMDLIARMLLDPGDDVLMED
                     PGYTGARGAWLAAGASVTPIPVDADGMMVTDGRAPDARLAYVTPSCQFPLGVPLSLER
                     RLALLDWARRAQAWIVEDDYDCDLRFDRQQLPCLHALDPDGRVIYVGTFSKTLFPALR
                     LGFLIVPRDLQSGFIAARLATELHPPVLEQRVLAAFIQRGHYERHLRRMQTAYAERLD
                     ALRRAVDRSGAPLRIRPVRAGMHAVLDVDGAGAERVHTVAAAAGVESMPLSS"
BASE COUNT          519 a         1068 c         1052 g          598 t
ORIGIN      
        1 cattcccgcc actgcggatt acttgtgaat ggacctccgg catacggagc aacaatcctc
       61 gccattggtt gcgggcccaa ctcgcaaatc tgatgcgagg aggccgttat tcaccgccgt
      121 cggagcgatc ttcaaggtca caaactgtga ccttagacga ctaagtcatt gatgggaaag
      181 ggcgcttgat gtcgcaatct gcgatatcaa gttgctcgcg attcatcagg caaacgtgat
      241 ccggctcctg aaaccgggat caagagtggg agtgcagcgt gaaatgcctt cagcttcggc
      301 ggtgagtgtc gccacgtcgg acaggatgtg gctcttgggg tggacgccac ctgcgtcatc
      361 ggcttcgaca tctgaagtag tttgacgccg gctccctgcg cggctcactt gcgggccacc
      421 gtcccgagtt cgaccgcatt cgcagactgg tcgcatggga tttcaccaga gtggttctac
      481 cacctccgag gtgcgcttct cagactcacc gcaactcaat ccaaggagag tgcgatgagg
      541 aaaatacaag cattgaagat gatgttcgcc tgcatgctcg cttcgctgtg gatgtccacc
      601 gcgttggcgc aattggcccc tgcgcaggcg gactcctcgc tttccggcgg caaccgctgg
      661 gttgcaacct ggggcgcggc gcccatcaat cccggcctga ccaccatcga tgccctcttc
      721 ctgaatgacc acagccgttc cttcgagaac cagaccgtgc gcaacatcgc gcatgtcagc
      781 gttggtggcc ggcgcgtgcg ggtgcgcgtg tccaatgcct ttggtgtcct gccgctgcgc
      841 ctgggcgcgg cgcatgtcgc cctcagccgc ggcggcgcgt ccatcaacgt caccaccggc
      901 cggcggctca ccttcggcgg gcaaacctcc gtcctgattc cggcgggtgc cgtcatggtc
      961 agcgatgccg tggacatgta cgtggcttcg gcctccgatc ttgcggtgag cctctatcta
     1021 ccgggggtca ccgagccggc gaccttcaac gagttcaaga tgcacacctc gtatgtcacg
     1081 ggcgtgaaca gcggcaacct ggtgagcgcc gccgacctgc ccacggccga ggcggtccgt
     1141 caggttttct acacgaccgt ggtggaagtg cagcccaccg aatccgtggg agcgctggtg
     1201 acgattggag attcgatcac gctgggcggg aactcgacac cggatgccta tcgcacctgg
     1261 ccggacctgt tgtcggagcg gatcaacacg cacaggcctc gactgtccgt ggtgaaccag
     1321 ggcatcggct gcaatcggct gctgtacgac ctctgcgggc ccagcggcgc ggcgcgtttc
     1381 gatcgtgacg tgctcgcagt caccggtgtg tcgcacgtga tcgtggcttt cggcatcaat
     1441 gacctgatga ttccttcgat cctcccgcaa ttcggcttcc cggaatttgc atcggagacc
     1501 gtcagcgcgc aggacatcat cgtcggcctg cagcagctca tcgtgcggtc gcggtcgcgg
     1561 ggcatccgcg tctacggcgc caccatcacg ccgaatggtt cgatcacggt tccgggtgcg
     1621 cataccccgg acaccgaagc gaagcgcacc gcggtgaatc gctggatccg caccagcggc
     1681 gccttcgatg gcgtggtgga tttcgacgcc gcggtgcgcg atccatccaa tccggcgcgc
     1741 ctgcttccgg cctacgacgc ggacggcgtg catctcaccg atgccgggca ccaggccatg
     1801 gccaacgcgg tgaatctgtc gatgctcttc tgatagcctg cgccgagctg gaaatggcgg
     1861 gcgctgttcc gaatcaggga cagcgcccgt cgctcgggag aatggatgcg cagagcaagc
     1921 ctgccgttgt tcatcgaacg cggcacaagg acgttgcagg aacagatcta ccgtggcatt
     1981 cgccagtcca tcctcgatgg actagttagt gccgaccggc gcctgccgtc cacgcgctcg
     2041 ctggccgcgg acctgggcgt ctcccgcacc acggcgctgc tcgcgctgga acagttgcgt
     2101 gccgagggct acctcattgc gcggccgggg tcgggcacct tcatctcgcc gcggttgccc
     2161 ggaccaccag ccatgccggt gaacgcgccc gggaaaccca tcgcacgtcc gccgttctcg
     2221 cggcgcggcc agttgctggc gcgcatgcag gcgccggacc ggcgcgccaa cgtgccgccg
     2281 cgggcatttc ggctcggtac gccggcgctg gatctgtttc catggcggct gtgggcgcag
     2341 atcacgcgtg agtgcctgcg agactgtgcc gccacacacc tcgactactc accgctcgcc
     2401 ggcctgcgcg cgctgcgtga agccatcgcc gagcagatgc aggcacgcgg cacgcgctgc
     2461 gaccccgacc aggtgcaggt ggtcgcaggc gcgcagcgcg gcatggacct gatcgcacgc
     2521 atgctgctgg atcccggcga cgatgtgctc atggaagacc cgggttatac cggcgcgcgc
     2581 ggcgcctggc ttgcggcggg agcatccgtg actcccatac ccgtggacgc cgacggcatg
     2641 atggtcacgg acggacgcgc gccggatgcc cggctggcgt atgtcacgcc ttcctgccag
     2701 ttcccgttgg gcgtgccctt aagtctcgag cggcgcctgg cattgctcga ctgggcgcgg
     2761 cgcgcgcagg catggatagt cgaggacgac tacgactgcg acctgcgttt cgatcggcaa
     2821 caactgcctt gcctgcacgc gctggatccc gacgggcggg tgatctacgt gggcaccttc
     2881 agcaagacgc tgttcccggc gctgcggctg ggatttctca tcgtgccgcg cgacctgcaa
     2941 tccggcttca tcgccgcgcg gctggcgacc gagttgcatc cgccggtatt ggaacaacgt
     3001 gtgttggcgg cgttcatcca gcgcggccac tacgaacggc atctgcgccg catgcagacg
     3061 gcctatgcgg agcggctcga cgccttgcgg cgcgccgtcg atcgttccgg agcgccgctg
     3121 cgcattcggc cggtgcgcgc cggcatgcat gccgtgctcg atgtcgatgg ggcgggtgcc
     3181 gagcgggtgc acaccgtggc cgccgccgcc ggtgtcgaaa gcatgccgct gtcgtct
//
LOCUS       DASQJS010000202         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1366195, whole genome shotgun sequence.
ACCESSION   DASQJS010000202 DASQJS010000000
VERSION     DASQJS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366195"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VEW08_08295"
     CDS             complement(<1..1069)
                     /locus_tag="VEW08_08295"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HYJ40775.1"
                     /translation="MNLILPLLAAMALAAPAFGDNPETQVLPLSGMGETGETPVYWDF
                     KLDAGRGSGKWSRIAVPSCWEQQGFGAYYYGTQGRGKPDDDPVIPKETGTYRREFQIP
                     ASWDGRDIHIVFEAAMTDTSVRINGEAAGSTHQGGFYRFSYDIAKLVKPGRNEIEVVV
                     SKESSNQSVNHAERRGDYWTFGGIYRPVWLEARPRNHIAWTAIDARADGSFHARAHLN
                     RAVPEGAKLRAQVFDGQGVPVGGPLTAEVIHGDTAIFNGSFSSPALWNAETPNLYSAR
                     FTLESDTGVVHERRDRFGFRTLEVRPRDGVYLNGRKIVLKGINRHSFDPKTGRTLTRE
                     QNYADVRLIKGANMNAVRMSHY"
     gene            complement(1066..2490)
                     /locus_tag="VEW08_08300"
     CDS             complement(1066..2490)
                     /locus_tag="VEW08_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase-like domain-containing
                     protein"
                     /protein_id="HYJ40776.1"
                     /translation="MKLSFAIVLLTCLGIGSCSGAAAAEKPRVLVLTDIENEPDDAMS
                     MVRFLVYSNQWDVEGLVATTSIHQKNKVAPWRIGEIVDAYDKVRDNLELHERGFPTAD
                     FLRSRIVEGRPDYGMAAVGKDMESPGSRLLIAAVDRDDPRPLWVPVWGGPNVLAQALF
                     HVRATRSPQDLRKFVDKLRVYTISDQDDSGPWIRKNFPTLFYIASPGFHGGGAYHHST
                     WSGISGDKFHGRFAGADFVIVDNPWLNDHIRSKGPLGAQHPLTKFMMEGDTPSFLSLI
                     DNGLNAPDHPDWGGWGGRYELYTPRMEKWFYEPETRPFWTNAEDEVFGIDGNWHTSNK
                     ATIWRWRQAYQNDFEARMDWTIKPYAAANHPPVPALGHPDRLNAKPGERMSLSAQGSS
                     DPDGDRLSYQWFYYPEAGSLIVATGRSGAPVAIDKSNERDASFVVPENFFKPGTMHVI
                     LAVTDSGTPALTRYRRVIVSVTQQ"
     gene            complement(2531..>3532)
                     /locus_tag="VEW08_08305"
     CDS             complement(2531..>3532)
                     /locus_tag="VEW08_08305"
                     /inference="COORDINATES: protein motif:HMM:NF045351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40777.1"
                     /translation="FAWDNSELATDLWLWLYFLRTGRADTFRFAEAMTRHTGEVDVHH
                     LGKFAPLGSRHNVMHWGCSAKQLRISTALNRRYYYFLTADERVGDLMREQIDAARTLR
                     TVLATRKLPGAESRQVDDAKGDFAHLGFGTDWGSIAGAWLTEWERTRDPKMRDRLVAS
                     MKSIAAQPKGFFTGGERMNLVTGAFDISKDQGLNVSHLSAAFGLPEVCAELIELLDIP
                     EFERAWLLYCELYNGTDEQQKAALGESFGSINLQQGHARLTAYAARRKNDAALAARAW
                     KELTEGRSGYGARQQFKANQIEAPAVLNPVDEAPGVSTNATAQWGLAAIECLAYARK"
BASE COUNT          596 a         1102 c         1149 g          685 t
ORIGIN      
        1 ggtagtgcga catgcgcacc gcgttcatgt tggcaccctt gatcaggcgc acgtcggcgt
       61 agttctgttc gcgcgtgagt gtgcggccag tcttgggatc gaagctgtgt cgattgatgc
      121 ccttgagcac gatcttgcgg ccattgagat agacgccatc gcgtggccgg acttcgagcg
      181 tacggaagcc gaagcgatcg cggcgctcgt ggacgacgcc cgtatcgctt tcgagagtga
      241 agcgcgcgga atacagattg ggtgtctcgg cgttccacaa cgcaggtgag gaaaacgagc
      301 cgttgaaaat agcggtgtca ccgtggatca cttcggcggt caatggcccg ccgacaggga
      361 cgccctggcc gtcaaacacc tgcgcgcgaa gcttcgcgcc ttcgggcacg gcgcgattga
      421 gatgcgcacg tgcatggaaa ctgccgtcgg cgcgcgcatc gatcgcggtc cacgcgatgt
      481 gattccgcgg ccgcgcctcc aaccacaccg gccggtagat gccaccgaag gtccagtagt
      541 cgccacgccg ctcggcgtgg ttcacgctct ggttggacga ctccttcgaa acgacgactt
      601 cgatttcatt tcgaccgggc ttcacgagtt tcgcgatgtc gtagctgaag cgatagaacc
      661 cgccctggtg cgtggatcca gcagcctcgc cattgattct tacgctcgtg tcggtcatcg
      721 ccgcttcgaa cacgatgtga atgtcgcgac cgtcccatga tgcggggatc tggaattcac
      781 gccggtacgt cccggtctcc ttcgggatga cgggatcgtc gtcgggtttg ccgcgtcctt
      841 gcgtgccgta gtagtaagca ccgaagccct gctgctccca gcacgacgga accgcgatgc
      901 gactccactt gccgctgccg cgtccggcat cgagcttgaa atcccagtag acgggcgttt
      961 cgccggtttc gcccatgccg gacagcggca atacctgggt ttccgggttg tcgccgaacg
     1021 caggtgcagc gagcgccatc gccgccagca aagggagtat gaggttcact gctgcgtgac
     1081 gctgacgatc acgcggcggt atcgcgtcaa cgctggtgtg ccactgtcgg tgaccgcgag
     1141 gatgacgtgc atggtgccgg gcttgaagaa attctccggc acgacgaacg aggcatcacg
     1201 ctcgttcgat ttatcaatgg cgacgggcgc gccactgcgg cccgtggcga cgatcagcga
     1261 tccggcttcc ggatagtaga accattgata ggagagccgg tcgccgtccg gatcgctgga
     1321 accttgcgcg ctcagactca tgcgctcgcc gggcttcgcg ttcaaccgat caggatgccc
     1381 gagcgctggc accggcggat gattcgccgc ggcgtatggt ttgatggtcc agtccatacg
     1441 cgcctcgaaa tcgttctgat aggcctggcg ccagcgccag atcgtcgcct tgttagacgt
     1501 gtgccagttc ccgtcgatgc cgaaaacctc gtcctcggca ttggtccaga acgggcgcgt
     1561 ctcgggttcg tagaaccatt tctccatgcg cggcgtgtac aactcgtagc ggccgcccca
     1621 gccgccccag tcgggatgat cgggagcatt gagcccgttg tcgatgagcg agaggaagct
     1681 cggcgtgtcg ccttccatca tgaacttggt gagcgggtgc tgtgcgccga gcggaccctt
     1741 cgagcggatg tgatcgttga gccacgggtt gtcgacgatc acgaaatccg cgccggcaaa
     1801 ccggccgtgg aatttgtcgc cgctgatgcc gctccacgtc gagtgatggt aggcgccgcc
     1861 accgtgaaag ccggggctcg cgatgtagaa gagcgtgggg aaattcttgc ggatccacgg
     1921 gccgctgtcg tcctggtccg agatcgtata aacgcgcagc ttgtccacga acttccgcag
     1981 gtcttgcggt gagcgtgtcg cgcgcacgtg gaacagcgcc tgcgccagca cgttcggtcc
     2041 gccccacacc ggcacccata gcggccgcgg gtcatcgcga tccaccgccg cgatgagcag
     2101 gcgcgagccc ggggattcca tgtcttttcc gacggcagcc atgccgtagt caggccggcc
     2161 ttcgacgatg cgcgagcgca ggaagtccgc ggtcgggaag ccgcgctcgt gcaattcgag
     2221 gttgtcccga accttgtcgt aggcgtcgac gatttcgccg atgcgccagg gtgcgacctt
     2281 gtttttctgg tgaatcgacg tggtcgccac cagcccttcg acatcccact ggttcgagta
     2341 cacgaggaat cgcaccatcg acatcgcgtc gtcgggctcg ttttcgatat cggtgagcac
     2401 gagcacgcga ggcttttcag cggctgccgc gccgctacag gagccgatgc caaggcaggt
     2461 cagcagcacg atcgcgaacg agagtttcat ggggcgcact gtaacgcggc cgcggatgtc
     2521 tggcccttat ctacttgcgc gcgtacgcaa ggcactcgat ggccgcgagc ccccactgcg
     2581 ccgtcgcgtt cgtcgagact ccgggcgcct cgtcgaccgg gttcagcacc gccggtgcct
     2641 cgatctggtt cgccttgaac tgctggcgcg cgccatagcc gctacgcccc tcggtgagtt
     2701 ccttccaggc ccgcgcagcg agggcggcgt cattcttgcg ccgggccgcg taagcggtga
     2761 ggcgcgcgtg cccctgctgg aggttgatgc tgccgaaact ttcgccgagc gcggccttct
     2821 gctgttcgtc ggtgccgttg tacagctcgc aatacagcag ccacgcgcgt tcgaattccg
     2881 gaatgtccag cagttcgatg agttcggcac acacttcggg cagaccgaag gccgccgaca
     2941 agtgcgatac attcaatccc tgatctttcg agatgtcgaa cgcgccggtg acgagattca
     3001 tgcgctcgcc gcccgtgaag aatcccttgg gctgtgcggc aatgctcttc atgctcgcga
     3061 ccagccggtc gcgcattttc ggatcgcgcg tgcgctccca ttcggtgagc caggcgccgg
     3121 cgatcgatcc ccagtcggtg ccgaacccca ggtgcgcgaa atcgcctttc gcgtcgtcca
     3181 cctggcgcga ctctgcgccc ggtagtttgc gagtcgccaa cacggtgcgc agcgtgcgcg
     3241 ccgcgtcgat ctgctcgcgc atgagatcgc cgacgcgttc gtcggcggtg aggaagtagt
     3301 agtagcggcg gttcaacgcc gtgctgatgc gaagttgctt tgccgagcag ccccagtgca
     3361 tgacgttgtg gcgcgagccg agcggcgcga acttgccgag gtggtgtaca tcgacctcgc
     3421 cggtgtggcg cgtcattgct tcggcgaacc ggaacgtgtc agcacggccg gtgcgcagga
     3481 agtagagcca cagccacagg tcggtggcga gctcggaatt gtcccaggcg aa
//
LOCUS       DASQJS010000203         9086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2313736, whole genome shotgun sequence.
ACCESSION   DASQJS010000203 DASQJS010000000
VERSION     DASQJS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9086
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2313736"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..2057
                     /locus_tag="VEW08_08310"
     CDS             144..2057
                     /locus_tag="VEW08_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ40778.1"
                     /translation="MNNARNETAAAAQATTDLPLQAQLMGSLALVFILSAVAGLVGYA
                     TFDTTSLERGVASKANVYASTLSSQGYNAVLSENPKLAESVIAPLLSDRNIYGVAIYA
                     AGGKLLAGHAHFPATLKNASEAKFADDRVLVTVRDVPVGYGASGHVLLALSTEHIASA
                     RFRAAQFPAMSTAVVLLFAILLALAHSFSVMMSEVVRMFEERRQMERTEKERLERLVS
                     ERTRELERGREQYRLVVEGTQAIPFALDVEDGHFEYIGPQGPQRLGFGEEVWKRIGFL
                     DKLMPRERNGPVRARIDESKSGNFEIETSVLTADDERVDLRWVASCEQSDNTGQHKVL
                     RGMMLDVTDQRRLENELAQAQKLESVGRLAAGVAHEINTPVQFVSDSVHFVREAMDDL
                     SGILGKYRELRTATQSPNQNGADIEAAAKAAEEAEDDADLDYILENAPVALDRARDGL
                     GRVAAIVRSMKEFAHPDRKEMAQADLNQAIASTLVIASNEYKYVAEVETDFAELPLVN
                     CFAGEINQVVLNLIVNAAHAIDDVVKGTPSKGLIRVSTRVLDDSVEIAISDTGKGIPV
                     EVRPRIFDPFFTTKEVGKGTGQGLAIARNVIVDKHGGTLRFETELGQGTTFYIRLPIA
                     GPVTDTETASAAA"
     gene            2066..3523
                     /locus_tag="VEW08_08315"
     CDS             2066..3523
                     /locus_tag="VEW08_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HYJ40779.1"
                     /translation="MKLAKVSTHHIQAVTGTTFVSTLSSLLFGYCTAVICGVVGAIQH
                     NFIEPRGMHETAANALLGLTVCAALGGTILGALMARRTAELLGRKKPMILASVLFLVS
                     ALGSAFPEAGLAPLGGMGPDAIWPFIVYRVIGGVAVGLASVIAPMYVAEFAPRAVRGQ
                     LGAYQQIAIAGGIAIVLFVNWGIALQGDDAWVLNTGWRYMMVSLAVPALAFFWLSFTV
                     PESPSWLVKHGRVEEARRSLSRSAEPEEVNVMLEDLAKSSGVQEKPAPLLAFGGRVVF
                     VGVALSVLQQLMGLNAISYYGPQILERMGFHMDAAFLGVLVARSLNLLATMVVVLIVD
                     RVGRKPLLIFGAMIMGLSMMALGALFENENTGVYGLIAMCCYMVALGISFGPIVWIMM
                     SEVFPAPIRGQAMSVAISAQWGANFLVSFTFPVMFGDSGLNAFGHGGFAFWIYGAFGL
                     LAAFVVLRFVPETKGIDGDQVAALWRRESFFKFSS"
     gene            complement(3527..4105)
                     /locus_tag="VEW08_08320"
     CDS             complement(3527..4105)
                     /locus_tag="VEW08_08320"
                     /inference="COORDINATES: protein motif:HMM:NF042672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6624 domain-containing protein"
                     /protein_id="HYJ40780.1"
                     /translation="MDESLEKALLEMEAQDQAVRNELTAAGALNDKYHPRLEEVHRAN
                     TSRLRQIIAVFGWPGITLVGDKGAKAAWRIALHAISEPPFMRQCRELLEHASDAGEAP
                     RWQFAIIDDRIRVYEGRPQRYGTQLRVGAKGLEPHPLENEARVSSMRAQAGLPPLAKT
                     LAQARAQPQPTPAEQAARDAAELEFRRTVGWI"
     gene            complement(4216..4482)
                     /locus_tag="VEW08_08325"
     CDS             complement(4216..4482)
                     /locus_tag="VEW08_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40781.1"
                     /translation="MNKLLATAVVALVPFAGIAAEGGKTQPATPAGQGGSFEAFDANK
                     DGRISLPEASADPKLVENFSAADKNGDGYLDNAEFDERAEPPQR"
     gene            4704..5975
                     /locus_tag="VEW08_08330"
     CDS             4704..5975
                     /locus_tag="VEW08_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYJ40782.1"
                     /translation="MAAFWFVLVGFLLVFIALLGRFLDRLPVSPAMIYLAVGFLLGPS
                     VFDVLELHPLRELTLLESITEIALLIALFTVGIKIRVPIGDWRWSVPLRLATISMVLT
                     ILGIAIVAVVLLDFDWRLALVLGAVLAPTDPVLASDVQLRSAQDRDSLRFGLTGEGGL
                     NDGTAFPCVLLGLGLMGLHELGFAGWRWLAVDVVWGTVGGLGIGFLLGSALGGGVRML
                     RAWRRDAAIVDEFLLLGAIALSYGVALAAHALGFLAVFAAGLALRRADDIHANSKNVA
                     DKSPLTPSLLNVNEQLERIVEVAVVLLVGAMISTGYWSPAGLLLAALLFVVIRPLAVW
                     IGVRGTDADGAPRRLLAWFGIRGIGSVYYAVFFAGYELRDAVATEVLSAVFTVIAASI
                     VLHGISATPLMELYRARRGRGRPPVAVSDKP"
     gene            6056..6247
                     /locus_tag="VEW08_08335"
                     /pseudo
     CDS             6056..6247
                     /locus_tag="VEW08_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
     gene            6328..6750
                     /locus_tag="VEW08_08340"
     CDS             6328..6750
                     /locus_tag="VEW08_08340"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HYJ40783.1"
                     /translation="MFTLNGIRNAFWCAIAFGIVACASTPMPIEKLAVARSSIERAEQ
                     AQAAQFAQIELDSARNKFAAAQTAADKRDAQVAARLADQADVDAQLAESTARARQQEQ
                     LVTEMDASLRDLRNETLRRDNTGTPAPQPAAQPFPQQP"
     gene            6761..7558
                     /locus_tag="VEW08_08345"
     CDS             6761..7558
                     /locus_tag="VEW08_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYJ40784.1"
                     /translation="MYRQRMKHSFVVVAASALLALAACETQPDPSAMLEQARSAITQA
                     EADPNVARYATTELDRARKLLVDAERAAGEKAEGGKTASHYAYLTTQMVRIAQQRAQE
                     QVAITHLRAGETERQKILLSTQPRPQAQVESERLAAQLENLQASQTSRGIVLTLDEGL
                     FDMSKAQLKPGAERSIDQITAFLNQNPDRRVQVEGFTDSQGTNDFNIEVSQTRADTVA
                     MAIIHRGIDAERVRAIGYGEGYQMNRRVEIIVSNSDAAIPGRTAGAP"
     gene            7555..8598
                     /locus_tag="VEW08_08350"
     CDS             7555..8598
                     /locus_tag="VEW08_08350"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYJ40785.1"
                     /translation="MKGEFLPGDHGASLALHEHAAPEGMEDCALRLIGNSPGMQKVRE
                     LISRVAPTDSTVLICGESGCGKELVAESVHAMSPRAGKPFVALNCAAIPATLIEAELF
                     GHERGSFTGALRTREGVFERANGGTLLLDEITEMPLDLQSRLLRVLETKRLSRVGGSA
                     DLPVDVRVIASTNRLPARAFEEGRLREDIYYRLAVLTIELPPLRDRGEDILVLAEFFL
                     GELNLRNGTRKRLSVELRERFARHRWPGNVRELRNAVERAYILCDGELNLSDQEMPSL
                     RGAEGAGADADGIRVNIGTTLEETERTLILATLRHFEGDKRRAADVLGCSLKTLYNKL
                     HAYKRGRQHAGMN"
     gene            8634..8903
                     /locus_tag="VEW08_08355"
     CDS             8634..8903
                     /locus_tag="VEW08_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HYJ40786.1"
                     /translation="MGIILWLVIGGVVGWLASIVMKRDGSQGIFLNIVVGIVGAMLGG
                     WLLGPMLGAPSINSGVISIGSLLVSFLGAVILLAIVNLISRSRVR"
BASE COUNT         1537 a         2863 c         3018 g         1668 t
ORIGIN      
        1 taccaatgca gttcctgaga cccagttcac agggcattcg acggcagaat cttgccgccg
       61 tccgggcctc cggatcgcac gctttccggg cgctgccatt caagggaaac ccgtccctta
      121 ccgataagtt ctgacggacc cttatgaaca acgcccgcaa tgaaaccgct gcagccgcac
      181 aggcgaccac ggacctgccg ctgcaggccc aactcatggg cagtcttgcg ctcgttttca
      241 ttctgagcgc ggtcgccggt ctcgtcggct acgccacctt cgacaccacc tcgctcgaga
      301 gaggcgtggc atccaaggcg aacgtatatg cgtcgacgtt gagctcccag ggctacaacg
      361 cggtgctgtc cgaaaacccg aaactggccg aatcggtcat cgcgccgctg ctgagcgacc
      421 gcaatatcta cggtgtggcg atctacgccg ccggtggaaa actgctcgcg ggtcacgccc
      481 actttcccgc gacgttgaaa aatgccagcg aagcgaaatt cgccgacgat cgggtgctgg
      541 tgacggtgcg cgacgtgccg gtcggctacg gcgcctcggg acacgtgctg ctggcactgt
      601 cgactgagca catcgcctcg gcgcgtttcc gcgccgcgca gtttccggca atgtccacgg
      661 ccgtggtgct gctgttcgcc atcctgctgg cgctggcgca ttccttcagc gtcatgatgt
      721 cggaagtcgt gcgcatgttc gaagaacggc gccagatgga gcgaaccgaa aaggaacgcc
      781 tcgagagact ggtatcggag cgcacgcgcg agctcgaacg cggacgcgag caatatcggc
      841 tggtggtcga aggcacccag gccattccgt tcgcgctcga tgtcgaagac ggtcatttcg
      901 aatacatcgg cccgcagggg ccgcagcgcc tgggctttgg agaagaagtc tggaagcgca
      961 tcgggttcct cgacaagctc atgccgcgcg agcgtaacgg cccggtgcgc gcgcgcatcg
     1021 acgagagcaa gtccggaaat ttcgaaattg aaacgagcgt actcacggcc gacgacgagc
     1081 gcgtcgacct gcgctgggtc gcgagttgcg agcagagcga caacacgggc cagcacaagg
     1141 tgctgcgcgg catgatgctc gacgtcaccg accagcgccg gctggagaac gagctcgcgc
     1201 aggcgcagaa actcgagagc gttggccggc tcgccgcggg cgtggcccac gaaatcaaca
     1261 cgccggtgca gttcgtttcg gacagcgtgc acttcgtgcg cgaagccatg gacgatctct
     1321 cgggcattct cggcaaatac cgcgagctgc gtaccgccac gcagagtcca aaccagaacg
     1381 gcgcggacat cgaggctgcg gcgaaggccg cagaagaagc cgaggacgac gccgacctcg
     1441 actacatcct cgagaacgcg cccgtcgcgc tggatcgcgc gcgagatggt ctcggccgcg
     1501 tcgccgccat cgtgcgctcc atgaaggaat tcgcgcatcc ggaccgcaag gagatggcgc
     1561 aggccgacct gaaccaggcg atcgccagca cgctggtcat cgcctccaac gagtacaagt
     1621 acgtcgccga agtcgaaacc gatttcgccg agctgccgct cgtgaactgt tttgcaggtg
     1681 agatcaacca ggtggtgctg aatctcatcg tcaacgccgc gcacgccatt gacgacgtgg
     1741 tcaagggtac gccctcgaag gggctgatca gggtcagcac tcgtgtgctc gacgattcgg
     1801 tcgagattgc tatcagcgac acgggcaagg gcattcccgt cgaggtgcgg ccgcgcatct
     1861 tcgatccgtt cttcaccacc aaggaagtgg ggaagggcac aggccagggc ctggccatcg
     1921 cacgcaacgt gatcgtcgac aaacacgggg gcacgctgcg attcgaaacc gaattggggc
     1981 agggcaccac gttttacatt cggctgccca tcgctggccc cgtgacggat accgaaaccg
     2041 cctcggccgc ggcctgaagg acatcatgaa actcgccaag gtctccactc atcacatcca
     2101 ggccgtgact ggcacgacct tcgtgtcgac gctgagctcg ttgctgttcg gctactgcac
     2161 ggccgtgatc tgcggcgtgg tcggcgccat ccagcacaac ttcatcgagc cgcgtggcat
     2221 gcacgagacg gcggcgaacg cgctgctcgg cctcactgtc tgcgccgcgc tcggcggcac
     2281 gatcctgggc gcgctgatgg cgcgcaggac cgcggagttg ttaggccgca agaagcccat
     2341 gatcctggca tcggtgctgt tcctggtctc ggcgctgggc tcggcgtttc cggaggcagg
     2401 actcgcgccc ctcgggggca tgggaccgga cgccatctgg ccgttcatcg tctatcgcgt
     2461 cattggtggc gtggccgtgg gcctggcgtc ggtgatcgcg ccgatgtacg tggccgaatt
     2521 cgcgccgcgc gcggtgcgag gccagctcgg cgcgtatcag cagatcgcga ttgccggcgg
     2581 catcgccatc gtgctgttcg tcaactgggg cattgcgctg cagggcgacg atgcgtgggt
     2641 gctcaacacg ggctggcgct acatgatggt gtcgctggcg gtgccggcgc tggccttctt
     2701 ctggctcagc ttcacggtgc ccgagagccc gagctggctg gtgaaacacg gccgcgtcga
     2761 agaagcgcgc cgttcgctgt cgcggtcggc ggaacccgaa gaagtgaatg tgatgctcga
     2821 ggacctggcg aagtccagcg gcgtgcagga gaagccggca ccgctgctgg ctttcggcgg
     2881 gcgggtggtg ttcgtcggcg tcgcgctgag cgtcctgcag cagctcatgg gcctcaacgc
     2941 gatctcctac tacggtccgc agatcctcga acgcatgggc ttccacatgg acgccgcatt
     3001 cctcggcgtg ctcgttgcgc ggtcgctcaa cctgctcgcg accatggtgg tcgtgctcat
     3061 cgtcgatcgc gtcgggcgca agccgctgct gattttcggc gccatgatca tggggttgtc
     3121 gatgatggcg ctgggagcgc tgttcgagaa cgagaacacc ggcgtctacg gcctgatcgc
     3181 gatgtgctgc tacatggtcg cgctcggcat ctcattcgga cccatcgtgt ggatcatgat
     3241 gtccgaggtc ttccccgcgc cgattcgcgg ccaggccatg tcggtggcca tttccgcgca
     3301 gtggggcgcg aatttcctcg tctccttcac gttcccggtc atgttcggcg acagcggcct
     3361 gaacgcgttt ggccacggtg gcttcgcgtt ctggatctac ggcgccttcg ggttgctggc
     3421 ggcgttcgtg gtgctgcgct tcgtccccga gacgaagggc atcgacggcg accaggtggc
     3481 ggcgctgtgg cgccgcgaga gcttcttcaa attctctagc tagagctcag atccagccga
     3541 cggtccggcg gaattcgagt tccgcggcgt cgcgcgcggc ctgttccgcg ggcgtcggct
     3601 gcggttgcgc gcgcgcctgc gccagggtct tggccagggg tggcaggccg gcctgcgcac
     3661 gcatggaact cacgcgcgct tcgttttcga gggggtgtgg ctcgaggcct tttgcgccca
     3721 cgcgaagctg tgtgccgtaa cgctgcgggc gtccctcata gacccggatg cggtcgtcga
     3781 tgatggcgaa ctgccagcgt ggcgcctcac cggcatcgga ggcgtgctcg aggagttcgc
     3841 ggcactggcg catgaacggc ggttcgctga tggcatgcaa cgcgatgcgc catgccgcct
     3901 tggcgccctt gtcgccgacc agtgtgatgc cgggccagcc gaacaccgcg atgatctgcc
     3961 gcagacggct ggtattggcg cgatgcactt cttcgaggcg cgggtgatat ttgtcattga
     4021 gagcgccggc ggcggtcaat tcgttgcgca ccgcctggtc ctgcgcttcc atttcgagaa
     4081 gtgccttctc gagtgattcg tccatgccct gcctggctcc cctcagctaa cagcgccgcc
     4141 aatatacacg acggggacgg cggggaaacc cgcgcggcac gaacaccgcg cggggcacga
     4201 acacacgctt ccaatctacc gctgcggcgg ttccgcgcgt tcgtcgaact cggcgttgtc
     4261 caggtagccg tcgccgttct tgtcggcggc ggagaaattc tcgaccagct tcgggtccgc
     4321 cgaagcttca ggcaacgaga tgcggccgtc cttgttggca tcgaacgcct cgaacgagcc
     4381 gccttggccg gcgggcgtgg ccggctgtgt ctttccacct tcggcggcga tgccggcgaa
     4441 cgggaccagg gcaaccaccg cggtggcgag aagtttgttc atgagtcact cctatgctgg
     4501 aagtctcttg cggcaacgac attgcactgg atcccccaag gggtccaact ggtggcgcag
     4561 agcgacagcc gctccaatac taactacaaa cagccacttg cgggccgggg cggaatgcgc
     4621 ttacgtcgaa cccgtgtgtt gtcccacgcg gatgtcagcg cattccgggg gcgcgacgcg
     4681 ccggcaactc tcaaggtgcg cagatggccg cattctggtt cgtgctcgtc ggatttctgc
     4741 tggtgttcat cgcgcttctg ggccgctttc tcgatcgcct tccggtgagc ccggcgatga
     4801 tttatctcgc ggtgggtttc ctgctcggcc cgtccgtatt cgatgtgctc gagctgcatc
     4861 cgctgcgtga gctcacgctg ctcgagtcca tcaccgagat tgcgctgctg atcgcgctgt
     4921 tcaccgtcgg catcaagata cgcgtgccca tcggcgactg gcgctggagc gtgccgttgc
     4981 ggctcgccac catctcgatg gtgctcacca ttctcggtat cgccatcgtg gcggtggtgc
     5041 tgctcgactt cgactggcgg ctggcgctgg tgctcggcgc tgtactcgcg ccaaccgatc
     5101 cggtgctcgc atcggacgtt cagctgcgca gcgcgcagga cagagacagt ctgcgtttcg
     5161 gtctcacggg cgagggcggt ctcaacgacg gaaccgcctt tccatgcgta ttgttaggcc
     5221 tgggattgat ggggctccat gaactcggct tcgcaggctg gcgctggctc gccgttgatg
     5281 tcgtgtgggg caccgtgggc ggattgggga tcggctttct cctgggatcg gcgctgggcg
     5341 gcggcgtgcg catgttgcgc gcctggcggc gcgacgcggc gatcgtcgat gaattcctgc
     5401 tgctcggcgc gattgcgctg agttacggcg tggcgcttgc ggcccacgca ttgggatttc
     5461 tcgcggtctt cgccgcaggg ctcgcgctgc ggcgcgccga tgacattcac gcgaattcga
     5521 agaatgtggc cgacaagtcg ccgctgactc ccagcctgct caacgtcaac gagcagctgg
     5581 agcgtatcgt cgaagtcgcg gtggtcctgc tggtgggcgc catgatctcc acgggctact
     5641 ggtcgccggc agggttgttg ctcgcggcgt tgttgttcgt ggtgattcgc ccgctcgccg
     5701 tgtggatagg ggttcgcggc acggacgccg acggcgcgcc acgccggttg ctggcatggt
     5761 tcggcatccg aggcatcggt tcggtgtact acgcggtgtt cttcgcgggc tacgaattgc
     5821 gcgatgccgt ggccactgag gtgctgtcgg cggtttttac ggtgatcgcc gcatccatcg
     5881 tcctgcatgg catatcggcc acgccgctca tggagctgta tcgcgcacgg cgcgggcggg
     5941 gcaggccgcc ggtggcggtg tcggacaaac catgacattg gcggcgtcgc gatcccacga
     6001 cgtacccggc ccctgccgct gtgcgcattg cggcacgaat tccagaatcg cgcccatgaa
     6061 caactgggat cggatttccg gcaactggat gcagtggaag ggccgtgttc gcgagcgttg
     6121 gggcaagttg accgacgatc agctcgatgt cgtggccggt cggcgcgagc agctgtccgg
     6181 ccgcatccag gacgcgtatg gcttgtcgaa ggaagaagcc gaacgccagc tgctcaattg
     6241 gcaacgcaat ctcgcggtcg aatatgacga agcggatctc gtgatcgatg aagacgagcg
     6301 cgacgtacag agcggacgga agtagccatg tttaccctga atggaattcg caacgccttc
     6361 tggtgcgcga tcgctttcgg catcgtggcc tgcgcctcca cgcccatgcc catcgaaaag
     6421 ctcgcggtcg ccaggagctc gatcgagcgc gccgagcagg cacaggccgc gcagttcgcg
     6481 cagatcgaac tggatagcgc gcgcaacaag ttcgccgcgg cccaaacagc cgccgacaag
     6541 cgcgatgccc aggtggcggc tcgcctggcc gaccaggctg acgtggatgc gcagctcgcc
     6601 gagtccaccg cgcgcgccag gcagcaggaa caactcgtca cggagatgga cgccagcctg
     6661 cgcgacctgc gcaacgagac actgcgccgt gacaacactg gcacgccggc cccgcagccc
     6721 gccgcgcaac cctttccgca gcagccataa gaggcactcg atgtatcgcc agcgcatgaa
     6781 gcactctttc gttgttgtcg cggcttccgc cttgttagcg cttgccgcgt gcgagaccca
     6841 gcctgatcca tccgccatgc tcgagcaggc gcgcagtgcc atcacccagg ccgaagctga
     6901 tccgaacgtt gccaggtacg cgaccactga gctcgaccgg gcacgcaagc tgctggtcga
     6961 cgccgagcgt gccgccggag agaaggccga gggcggcaag accgcgtcgc actacgccta
     7021 tctcacgaca cagatggtgc gcatcgcgca gcagcgtgca caggagcagg tggccatcac
     7081 gcacctcagg gccggagaga ccgaacgcca gaagattctg ctctcgacac agccacggcc
     7141 acaggcacag gtggagtccg agcggctggc cgctcagctc gaaaacctgc aggcctcgca
     7201 gacttcccgc ggaatcgtgc tgacgctcga tgaagggttg ttcgacatga gcaaggcgca
     7261 attgaagccg ggagcggagc gttcgatcga ccagatcacg gcgtttctca accagaaccc
     7321 ggatcgtcgc gtacaggtgg aaggattcac cgatagccag ggcaccaacg atttcaacat
     7381 cgaggtttcc cagacccggg cggatacggt ggccatggcc atcatccacc gaggaatcga
     7441 tgccgaacgc gtgcgtgcga tcggttacgg cgaggggtac cagatgaacc gtcgcgtgga
     7501 gatcatcgtc tccaacagtg atgccgcgat cccaggccgc acggccggag ccccgtgaag
     7561 ggcgagttcc tgcccggaga tcatggcgcg tcacttgcgc tgcacgaaca cgctgcgcct
     7621 gaaggaatgg aagactgcgc gctgcgactc atcggcaact cgccgggcat gcaaaaggtc
     7681 cgcgagctca tatcgcgggt ggcgccaacc gattcgacgg tcctgatctg cggcgagagc
     7741 ggctgcggca aggaactcgt cgccgaatcg gtgcacgcca tgagtccgcg cgctggcaag
     7801 ccgtttgtcg cgctcaactg cgccgcgatc ccagcgacgc tcatcgaggc cgaactattc
     7861 gggcatgagc gaggaagttt cacgggtgcg ctgcgtaccc gcgaaggagt cttcgagcgc
     7921 gccaacggcg gaaccctgct gctcgatgag atcacggaga tgccgctcga cctgcagtca
     7981 cgcctgttgc gcgtgctgga aaccaagcgg ttgagccgcg tgggcggctc cgcggatctg
     8041 ccggtcgacg ttcgggtcat cgcctccacg aaccgcctgc cggcgcgtgc attcgaagaa
     8101 gggcgattgc gcgaggacat ctactatcgc ctggccgttc tgaccatcga gctgccaccc
     8161 ctgcgcgatc gtggtgagga catcctggtg ctcgcggagt tcttcctcgg ggagctcaac
     8221 ctgcgcaacg gcactcgcaa gcgtctgtcg gtggaattgc gggagcgctt cgcccgccat
     8281 cgctggccgg gcaatgtgcg cgaattgcgc aacgcggtgg agcgggcgta catcctgtgc
     8341 gatggcgagc tgaatctcag cgaccaggaa atgccctcgt tgcgcggcgc cgaaggcgcg
     8401 ggcgcggacg ctgacggaat ccgcgtgaat atcggaacga cgctcgaaga aacagagcgc
     8461 actctcattc tcgccacgct ccgccatttc gagggcgaca agcggcgtgc cgccgatgtg
     8521 ctcggctgca gcctgaagac gctttacaac aagctgcacg cgtacaagcg cgggcggcag
     8581 catgccggca tgaactgaac aacgcgcgca accgcgcgca caaggagaga gcgatgggta
     8641 tcattctgtg gctggtcatt ggcggtgtgg tgggttggct cgccagcatc gtgatgaagc
     8701 gcgacggcag tcagggcatc ttcctgaata tcgtcgtggg tatcgttgga gcaatgctgg
     8761 gcggatggct gctgggcccg atgctcggcg cgccttccat caactccggt gtcatcagca
     8821 tcggtagttt gctggtgtcg ttccttggcg cggtgatcct gctcgccatc gtcaacctca
     8881 tctcgcgcag tcgcgtgcgc tgatcgcggc ccggcgccgc gcggcgggag ctcgagcatc
     8941 gtggtcgcgg acctctttga agtccacgtc ccggtgctgg agctcgtgct gcgcggcacg
     9001 ctggtgtatt ggctgctgtt cctgattttt cgcttcctgc tgcgccgtga cgtcggcgcg
     9061 gtcggcatcg ccgacatcct gctgct
//
LOCUS       DASQJS010000204         3446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_115012, whole genome shotgun sequence.
ACCESSION   DASQJS010000204 DASQJS010000000
VERSION     DASQJS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115012"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VEW08_08360"
     CDS             complement(<1..710)
                     /locus_tag="VEW08_08360"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HYJ40787.1"
                     /translation="MAAPTQTVLVLGAYGFFGSRICAALAKNPRIRLILAGRSLSKAT
                     AAAYQIGLSANHARALDATRTDLALQLRKLGVNTVIHTAGPFQGQGYAVARAAIKAGC
                     NYFDLADGRAFVGGVTKLDAEARAAGVSIISGVSSLPALTSAVVDKYRGEFKRLDIIR
                     IGITSGAVVPGLATIRSVLGYCGKPFRTLENGAWIDVHGWLDTQEYDFSRSVGVRRIG
                     RCDVPDLDLLPQRYPGVKT"
     gene            complement(720..1220)
                     /locus_tag="VEW08_08365"
     CDS             complement(720..1220)
                     /locus_tag="VEW08_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40788.1"
                     /translation="MNARAASMMTLLCAAGCALALAHDAPNSLIKLDFVGDSVRAEVL
                     VPESELAFATAAEHVSEPFADYLLRHLSAETPQGTPWKVDVRSVSNVTYLGHAYLSAQ
                     VEFTPPPGASPRSFVLIDDAVTHEVRNHVVLVLARGAANREVLGVLQYPARRLVVQRL
                     SATAQK"
     gene            complement(1217..2311)
                     /locus_tag="VEW08_08370"
     CDS             complement(1217..2311)
                     /locus_tag="VEW08_08370"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HYJ40789.1"
                     /translation="MKLPWPAAMVGLLMVVNAAHPADSLCPRPSTGDGQVQAVITAVK
                     ALQKSLTAEQRKALEQPFVRDSAIRWSNLPVGVVPRTGLRLGDLDVKQEYAARRVAAA
                     ALSACGLKMLDEIRIADNFLKNVDARKIGWDGANYFMSILGTPSEKEPWMLQIGGHHI
                     AYNLTFNGGREGATPLFFGSEPIYFNMGDTDYEPLVTQSAAMSTLARALANHAEAKLS
                     GTFTDVVKGVVVEMVQGQLPKGGTDTGFPMSYPTGTTDRGILYGALSAEEQALVRTAL
                     ETYASLPGDAITEELVAAYESPAALAETYVGYSGQPDLTAEGSYVRIDGPRIWMELIV
                     QRAVADRTKLHYHALWRDKQSDYGGEIGAR"
     gene            complement(2308..2730)
                     /locus_tag="VEW08_08375"
     CDS             complement(2308..2730)
                     /locus_tag="VEW08_08375"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HYJ40790.1"
                     /translation="MKFFTALLAGLIFGAGLLVSGMTNPANVLGFLDIFGEWRPQLAI
                     VMGAAVLTAAPSFWAVRRSRRSLLGAPVDLPDRRHIDKLLLAGAATFGVGWGLSGICP
                     GPGIVLLASGHGMAYLFMAAVIAGMWLVQLILNKRALR"
     gene            complement(2727..3146)
                     /locus_tag="VEW08_08380"
     CDS             complement(2727..3146)
                     /locus_tag="VEW08_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HYJ40791.1"
                     /translation="MTPLNSPEVLRAIAGGMLIGAGTAALLLLNGRVAGVSGILGNVI
                     REAAGEQGWRLGFLLGLLVPALILGTGPALLPHHWAWATGSGLLVGAGTQIGSGCTSG
                     HGVCGLANLSTRSLAATLVFMTAAVLTVLVVRHGGLQ"
     gene            3338..>3446
                     /locus_tag="VEW08_08385"
     CDS             3338..>3446
                     /locus_tag="VEW08_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HYJ40792.1"
                     /translation="MARGINKVILIGNLGADPETRAMPSGMTVANIRVAT"
BASE COUNT          648 a         1109 c         1113 g          576 t
ORIGIN      
        1 gttttcacgc ccggatagcg ctgcggcagc aaatcgagat cgggtacatc gcaacggccg
       61 atccgccgta cgcccacgga cctcgagaag tcgtattcct gcgtgtcgag ccagccgtgc
      121 acgtcgatcc acgcgccatt ctccagcgta cggaaaggct tgccgcaata gcccaggacc
      181 gaacgaatcg tcgcgagtcc cggaacgacc gccccggaag tgattccgat gcgaatgatg
      241 tcgaggcgtt tgaactctcc gcggtatttg tcgacgaccg cagaggtgag cgccggcaaa
      301 gagctcacgc cgctgatgat cgagactccc gccgcacgtg cttccgcatc cagcttcgtg
      361 acgccaccga cgaatgcgcg gccatcggcg agatcgaagt agttgcagcc ggcttttatc
      421 gccgcccgcg ccaccgcgta gccttgaccc tggaacggac ccgcggtgtg gatcacggtg
      481 ttgacgccca gcttgcgtag ttgcagcgcg aggtccgtgc gggtggcgtc cagcgcgcgt
      541 gcgtggttgg cactcaggcc gatctgatac gcggcggcgg tggccttgga gagactgcgg
      601 cccgcgagaa tgagccggat acgagggttc ttcgccagcg ctgcgcaaat ccggctgccg
      661 aaaaatccat acgcgccgag cacgaggact gtctgggtgg gtgccgccat tccgcgcgac
      721 tacttttgtg ccgtcgcgga gaggcgttga accacgaggc gccgcgcagg gtattgcagc
      781 acacccagga cttctcgatt cgcggcgccg cgcgccagca cgagaacgac gtggttgcga
      841 acttcatgcg tgacggcgtc gtcgatcaat acgaaagagc gtggcgacgc gcccgggggc
      901 ggcgtgaatt ccacctgcgc cgatagatag gcgtgaccga ggtaggtgac gttgctcacg
      961 gagcgcacgt ccaccttcca cggcgtgcct tgcggggttt ccgccgagag gtgacgcagc
     1021 aggtagtccg cgaagggttc cgacacgtgt tccgcggcgg tggcgaacgc gagctcggat
     1081 tccggcacca atacctcggc gcgaaccgag tctcccacga agtcgagctt gatcagcgaa
     1141 ttgggggcgt catgcgccag cgcgagtgcg catccggcgg cgcacagcaa cgtcatcatc
     1201 gatgcagctc gcgcgttcat ctggcgccga tctccccgcc atagtcggat tgtttgtcgc
     1261 gccacagcgc gtggtagtgc agcttggtgc gatcggccac ggcgcgctgg acgatcagct
     1321 ccatccagat acgcggccca tcgatgcgta cgtaagagcc ttccgcggtc aagtccggct
     1381 gacccgaata accgacataa gtttcggcga gcgccgccgg agattcatag gccgcaacca
     1441 gctcctccgt gatggcatcg ccgggtagag aggcgtaggt ttcgagcgcc gtgcgcacca
     1501 gcgcctgctc ctcggcggac agcgcgccgt agaggatgcc gcgatcggtc gtgcccgtgg
     1561 ggtacgacat cggaaatccc gtgtccgtgc cgcctttggg cagctgtccc tgaaccatct
     1621 cgacgaccac acctttcacg acatcggtga acgtgcccga tagtttcgcc tcggcgtgat
     1681 tcgccagcgc gcgcgccagc gtcgacatgg ccgcgctttg cgtgaccagc ggctcgtaat
     1741 ccgtatcgcc catattgaag tagatgggct cgctgccgaa gaacagcggt gtcgcgcctt
     1801 cgcggccgcc attgaacgtg aggttgtacg cgatgtggtg cccgccaatc tgcagcatcc
     1861 atggctcctt ttcggaaggt gtgccgagga tgctcatgaa ataattggcg ccgtcccagc
     1921 cgatcttgcg tgcatcgacg ttcttcagaa aattatcggc aatgcggatt tcgtcgagca
     1981 tcttgagtcc gcaggccgac agcgccgcgg ccgcgacgcg gcgcgccgcg tactcctgct
     2041 tcacatccag gtcgccgagg cgcaggccgg tgcgtggcac gacgccgact ggaagattcg
     2101 accagcgaat cgccgagtcg cgcacgaagg gctgctcgag ggctttgcgt tgctcagcgg
     2161 tgagcgattt ctgcagggcc ttgaccgcgg tgatgaccgc ctgaacctgg ccgtcgcccg
     2221 tggacggcct ggggcacagc gagtcggccg ggtgcgccgc gtttaccacc atcagcagtc
     2281 cgaccatcgc cgctggccaa ggtagtttca tcgaagggcc ctcttgttga gtatgagctg
     2341 aaccagccac atgcccgcga tcaccgccgc catgaacagg tacgccattc catggccgct
     2401 ggcgagcagc acgataccgg gtcccgggca gatgccgctc aggccccagc ccacaccaaa
     2461 cgtcgccgcg cctgcgagca gcagtttgtc gatatggcgg cgatccggca aatcgaccgg
     2521 ggcgccgagc aggctgcgcc ggctgcggcg caccgcccag aacgagggcg cggcggtgag
     2581 cacggccgcg cccatgacga tggcaagctg cggccgccac tcgccaaaga tgtcgagaaa
     2641 gcccagtacg ttggcggggt tcgtcattcc ggagacgaga agaccggcac cgaaaatcag
     2701 cccggcgagc agcgccgtga agaatttcac tgcaaacccc cgtggcgtac gacgagcacg
     2761 gtgagcacgg ccgcggtcat gaaaacgagc gtcgcggcca gcgagcgagt agatagattc
     2821 gccaggccgc agacgccgtg cccgctggtg cagccgctgc cgatctgcgt cccggcgcca
     2881 accagcaggc ccgaaccggt cgcccaggcc cagtgatgcg gcaatagcgc cgggccagtg
     2941 cccaggatga gcgccggcac cagcaggccc agcagaaagc caaggcgcca tccctgttcc
     3001 ccggccgcct cgcggatcac gttgccgaga atgccgctga cgccggcgac gcggccgttg
     3061 agcagcagca gtgccgccgt gcccgcgcca atgagcattc cgccggcaat ggcgcgcagg
     3121 acttcaggtg agttcagagg agtcacttgg gctttcgcat gaatttcgac aggttacgcg
     3181 cacaagctgc agggtagaag aagctgctcg actcgaagga gtggtcaaga ttcgtacaat
     3241 ctttgcctct gcatttgccg agaaacggcg atacatcttc gagtccgctc cccgtacaat
     3301 cccggcccac aataactttc gatgaagggc attcgccatg gcgcggggaa taaacaaagt
     3361 cattctgatc ggcaatctgg gcgcagaccc ggaaacccgc gccatgcctt cgggcatgac
     3421 ggtggcgaac atccgcgtgg ccacca
//
LOCUS       DASQJS010000205         5732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1683777, whole genome shotgun sequence.
ACCESSION   DASQJS010000205 DASQJS010000000
VERSION     DASQJS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683777"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..896)
                     /locus_tag="VEW08_08390"
     CDS             complement(45..896)
                     /locus_tag="VEW08_08390"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ40793.1"
                     /translation="MKKRINLQRTFNASLDLVWELWTTKEGIESWWGPPGFEVKVSKL
                     ELRAGGALEYVMNCIGAEQIAFMKQHNQPLSTPLKARYTEVSPKTLAAWMNLADFIPG
                     VVPYEVETRLMLAPLGAESVRVDLQLDAMHNEFYTSMATQGWEGEMGKLAAIIDQRSN
                     KDKFMSPLIPFLRFNDQAEEAARYYVGIFPGAKMGEIQRFPANSRGPEGGVMTASFML
                     GDQMLVALNGNPRFGFSPAMSLLFTCASSDEAARLTDQLAAGGEKQPGGWLRDKYGVS
                     WQVHANR"
     gene            complement(893..1237)
                     /locus_tag="VEW08_08395"
     CDS             complement(893..1237)
                     /locus_tag="VEW08_08395"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYJ40794.1"
                     /translation="MNISTFAALADPMRLALIEALRDGEQPVNELVERVHIQQSGVSR
                     HLGILSRAGLVSVRPEGQQRFYSLRQQPFDELDEWIARIRAHWEARLDRFGAELERRK
                     MAASTRKKRSNK"
     gene            complement(1329..1685)
                     /locus_tag="VEW08_08400"
     CDS             complement(1329..1685)
                     /locus_tag="VEW08_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HYJ40795.1"
                     /translation="MSDAGERDATAVRTRLVDLTRKFVTRTTGDVAQMRDALARLEAV
                     HGVVDLTALEEIHHLAHRACGTGGTLGLCELSDAAAALERLVEACPADAQPDAAVRAE
                     IAARIDALATQLDLLQ"
     gene            complement(1682..3088)
                     /locus_tag="VEW08_08405"
     CDS             complement(1682..3088)
                     /locus_tag="VEW08_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ40796.1"
                     /translation="MTHVKPVLDARADESAITQIQPVLVTIAPRVLLVDDDEIVIERL
                     RDLITAAGFEVATASDGAGALTELKRQFAPIVIMDRNMPGMDGLELTRRIRDDAQYPG
                     YVYIVLCTAHDSEDEILTGLKAGADDYISKRVSGTQLLARLATARRILSLEHSLKLVI
                     EERRRMAMTDSLTGAHNRRYFMSHMRRELKRARRLGGDLSLLVCDIDHFKHINDRHGH
                     SAGDDVLVEFVRRVQESLPREYDWCARMGGEEFAIVLPQTPLAGAATVAEKIRHAVAA
                     APMPTSAGNVEVTVSVGVTSLSVLTDPGAATAEQLLRRADDCLYTSKNHGRDRVTLDG
                     TPAPSVATGNALKSLLYVDDDADIREIVQMSLSLDGDLEVHTSDGGERALLKMRVERP
                     DLVVLDVMMPGMDGPTLLRRMRSDPELARIPVIFMTAKAGSSETGRLREMSAIGIIAK
                     PFDPMTLGRQVKALWEAH"
     gene            3279..>5732
                     /locus_tag="VEW08_08410"
     CDS             3279..>5732
                     /locus_tag="VEW08_08410"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HYJ40797.1"
                     /translation="MLATLYVVTGKLGLLLAVPPGYATIIWPASGIAIGMLLVHGARL
                     WPAILLGSWLLNAYQSGVFADGGWLSAKSFAALCIALGSTAQALAGRVLVARLIGVPL
                     RLKSARDVLLLLLLTGPVTCTIAASVGVSTLFALGIIGGESIAGNWIAWWSGDSLGVL
                     VFTPLVLLAPGGRAQITWRGKAIGQLPFAAMLLLALPLGLTFYAWQVISENDYLRGDA
                     KFETLSIAGEKALENRLASYGNALLGAASFIQGSSDVSSAEWRAYAETIRLRENFPGL
                     SGLAWVQPIETAALPDFVRRRRADGAPNFTIHPLLVGLPNYIVTFVEPEDENRAALGL
                     NIAFEDKRREAANLARDSGRPAITGLVMLVQDERSTPAFLMLYPIYHRNMPTGTVAER
                     RAAFRGWTDGPLLARNFLASLTDRQGSDYRLRVYDGNAELPEALAYTSGAANSSQPAF
                     VKRGTVQIMQHQWLLVWESTPAFELAQRSTNPLYILVGGLLFTALLSLLVVVLTVRRT
                     EHIEQMVGERRFIVPSLVFLLLTVGSFALYSRLLDQELEFVRRQVQNDSAEIDSQLRV
                     RTAEHIATLARMAARWKSAGGTNFDLWRSDATNLLNAYPGLRALQWIDSTHHVRWVEP
                     LAGNEGELERNVLGGDIREGALRAAAETSRELLTAPMQTRQGYAGFTAYLPVRREGRP
                     DGFIAGLFSVPNLFNGAVETRLSGDYAISIQFGGATVYANSRGDSPLTRGLATEKDLR
                     IGGRTWKLRMTPTPDFVERQKSGLPLLVLGAGLLVAALAALSVRFVLISRLKSAHLAK
                     SLALNAGIISSSAHLVIAID"
BASE COUNT          928 a         1899 c         1835 g         1070 t
ORIGIN      
        1 ggtacctacc gggaatacgc tcggggacgt cgatttgttc catctcatcg attcgcgtgt
       61 acctgccacg acacgccgta cttgtcgcgc agccagccgc ccggctgctt ctcgccaccg
      121 gcggcgagtt gatcggtcaa cctggcggcc tcgtcactgc tggcgcaggt gaacagcagc
      181 gacatggccg gtgagaatcc gaaacgcggg ttaccgttga gcgccacgag catctggtct
      241 cccagcatga agctcgcggt catgacgccg ccctcggggc cgcgactgtt ggcggggaaa
      301 cgctggatct cgcccatctt cgcgccggga aaaatgccca cgtaatagcg ggcggcttct
      361 tcagcctggt cgttgaaccg taaaaaagga atcagtgggc tcatgaattt gtccttgtta
      421 gacctttggt cgatgatggc ggcgagcttg cccatttcgc cctcccagcc ctgcgtcgcc
      481 atcgaggtgt agaactcgtt gtgcatggcg tcgagctgga ggtcgacgcg aacggattcg
      541 gcgccaagtg gggccagcat cagccgcgtc tcaacctcat acggcacgac gccgggaatg
      601 aaatccgcga gattcatcca cgccgccagc gttttcgggc tgacttccgt gtaacgcgcc
      661 ttgagcggcg tggacagcgg ctggttgtgc tgcttcatga acgcgatctg ttccgcaccg
      721 atgcaattca tgacgtattc gagcgcgcca cccgcgcgca gctcgagctt gctgaccttc
      781 acctcgaagc cgggcggacc ccaccaggac tcgatgccct ctttcgtggt ccagagttcc
      841 cagacgagat ccagcgatgc gttgaacgta cgctgcaggt tgatgcgctt cttcacttgt
      901 tgctccgttt tttcctcgtt gaggctgcca tctttcgtcg ctcgagctcg gcgccgaagc
      961 ggtcgagtcg cgcttcccag tgggcgcgaa tgcgcgcgat ccattcgtcg agctcgtcga
     1021 atggctgctg gcgcagggaa tagaaacgtt gctgaccctc ggggcgcacg ctgacgaggc
     1081 cggcgcgact caggattccg aggtgccgcg acacgcccga ctgctggatg tgcacgcgct
     1141 cgacgagctc attgaccggc tgctcgccgt cccgcagcgc ttcgatgagc gcgagacgca
     1201 tgggatcggc aagcgcggcg aaggtcgata tattcattaa tgtgtatata cacattcacg
     1261 gcttcaccag tcaaccagaa gattcggatg tcccgggcat gggacgcgga agtcggcagc
     1321 ggctttcgct actgcagcaa gtcgagctgc gtggccaggg cgtcgattcg cgcggcaatc
     1381 tccgcgcgaa ccgcggcgtc cggctgcgcg tcggcgggac aggcttcgac cagtctttcg
     1441 agcgcggccg ccgcatcgct cagctcgcac aggccgagcg tgccaccggt gccgcaggcg
     1501 cgatgcgcga ggtggtggat ctcctcgagc gcggtgagat ccactactcc atgcacggcc
     1561 tcgagccgcg caagcgcgtc gcgcatctgc gccacgtcgc cggtggtgcg cgtgacgaac
     1621 ttgcgtgtga ggtcaaccag gcgcgtcctg actgccgtcg catcgcgctc gccggcgtcg
     1681 ctcaatgtgc ctcccacagc gctttcacct gcctgcccag cgtcattggg tcgaatggtt
     1741 tggcaatgat gcctatcgcg gacatctcgc gcaggcgtcc ggtctcgctc gaccccgcct
     1801 tggcagtcat gaagatgacc gggatgcgcg caagctcggg gtcggaccgc attctcctga
     1861 gcagggtcgg cccgtccatg cccggcatca tcacgtcgag caccaccaga tcgggtcgct
     1921 cgacgcgcat cttcagcaat gcgcgctcgc cgccgtcgct cgtgtggacc tcgaggtcgc
     1981 cgtcgaggct cagtgacatc tgcacgatct cgcgtatgtc cgcgtcgtcg tcgacgtaca
     2041 gcagggactt gagcgcattg ccggtggcca cgctcggcgc aggcgtgcca tcgagcgtga
     2101 cgcgatcacg cccgtggttc ttgctcgtgt agaggcagtc gtcggcacgc cgcagcaact
     2161 gttccgcggt ggcggcgccg ggatcggtga gtaccgacag gctggtcact cccacgctca
     2221 cggtcacttc gacgttgccg gcgctggtcg gcatcggcgc ggcggccact gcatgacgga
     2281 tcttctcggc gaccgtcgcg gcaccggcga gcggcgtctg tggcagcacg atcgcgaatt
     2341 cctcgccgcc catgcgcgcg caccagtcgt attcgcgggg cagcgactcc tgcacgcggc
     2401 gcacgaattc caccagcacg tcgtcgcccg ccgaatggcc gtgacgatcg ttgatgtgtt
     2461 tgaagtgatc gatgtcgcac accagcagcg agaggtcgcc gccgagacgg cgcgcgcgct
     2521 tgagctcgcg tcgcatgtgg ctcatgaaat agcgccggtt gtgcgcgccg gtgagtgaat
     2581 cggtcatcgc catgcggcgc cgctcttcga tcacgagctt gagcgaatgc tcgagcgaaa
     2641 gaatgcgccg tgccgtggca agccgcgcca gcagctgcgt gccggagacg cgcttggaaa
     2701 tgtagtcgtc ggccccggcc ttgaggccgg tgaggatctc gtcttccgaa tcatgagcag
     2761 tgcacaacac gatgtagacg tacccggggt actgcgcgtc gtcacggatc ctgcgcgtga
     2821 gctcgaggcc gtccatgccc ggcatgttcc gatccatgat cacgatgggc gcgaactggc
     2881 gcttgagctc ggtgagcgcc ccggcgccat cgctggcggt cgccacttcg aagccggcgg
     2941 cggtgatcag gtcccggaga cgctcgatga caatttcgtc gtcgtcgacc agcagcacgc
     3001 gcggcgcgat cgtcaccagc acgggttgaa tctgcgtgat ggccgattca tcggcgcgcg
     3061 catcgagcac gggcttgacg tgcgtcacgg ggttacgcca ccgatcgcca actgcaagtt
     3121 gttgtcgcct tcacggacta ccatcgcatc tcacgttaac gtagcgagtg tacgagggat
     3181 aacggcgccg tcacagaagg cgcgacaccg cgccatcatg caaacgacta agggttttca
     3241 ccattccacc ctgggctaac tacctcggcc gggccgcgtt gctcgccacg ctgtacgtgg
     3301 tcacgggcaa gcttggcctg ctgctcgcag tgcctcccgg ctacgcgacg atcatctggc
     3361 ccgcgtccgg catcgccatc ggcatgctgc tggtgcacgg cgcgcggctg tggccggcca
     3421 tcctgctcgg ctcctggctg ctgaacgcct atcagtccgg cgtgttcgcc gacggtgggt
     3481 ggctgtcggc caagtccttc gcggccctgt gcatcgcgct cggatccacc gcgcaggcgc
     3541 ttgccggccg cgtcctcgtc gcgcgcctga tcggcgtgcc gctgcgcctc aagtcggcgc
     3601 gcgacgtatt gctgttgttg ctgctcaccg gcccggtcac ctgcaccatc gccgcgtccg
     3661 tcggcgtcag cacgctgttc gcgctcggca tcatcggtgg cgagtcgatc gccggtaact
     3721 ggattgcctg gtggtccggc gactcgctcg gtgtactcgt attcacaccg ctggtgttgc
     3781 tcgcccccgg cggccgcgcg cagatcacct ggcgtggaaa agcgattggc cagcttccat
     3841 tcgcggcgat gctgctgctg gcgctgccgc tcggcctgac gttctacgcg tggcaggtga
     3901 tctccgaaaa cgactacctg cgcggcgatg cgaagttcga gaccttgtcc atcgcgggag
     3961 aaaaggcgct ggaaaaccgc ctggcgtcct acggcaacgc gctgctcggc gccgccagct
     4021 tcatccaggg ctcctccgac gtatcgagcg cggaatggcg cgcctacgcg gaaaccatcc
     4081 ggctgcggga aaattttcct ggcctgagtg gcctggcgtg ggtgcagccg attgaaaccg
     4141 ccgccctccc cgatttcgtg cgtcgccgcc gcgcggacgg cgcgccaaac ttcacgatcc
     4201 acccgctcct ggttggcctt cccaactaca tcgtcacctt cgtcgaaccg gaggacgaga
     4261 atcgtgcggc gctcggcctc aacatcgcct tcgaagacaa acggcgggag gccgccaacc
     4321 tggcgcgcga ctccgggcgc ccggccatca cggggctggt gatgctggtg caggacgaac
     4381 gcagcacacc ggctttcctg atgctctatc cgatctacca tcggaacatg ccgaccggca
     4441 cggtggccga gcgtcgcgcc gcctttcgcg gctggaccga cggaccgctg ctggcgcgca
     4501 attttctcgc ttcgctgacc gaccgccagg gctccgacta tcgcctgcga gtctacgacg
     4561 gcaacgcgga acttccggaa gcactggcct acaccagcgg tgcggcgaac tcctcgcaac
     4621 ccgcattcgt caagcggggc accgtgcaga tcatgcagca tcagtggctg ctggtatggg
     4681 agagcacgcc ggcattcgag ctggcgcagc gatcgaccaa tccgctgtac atcctcgtcg
     4741 gcggcctgct attcaccgcg ctgttgtcat tgttggtggt ggtgctgacg gtgcggcgca
     4801 ccgaacacat cgaacaaatg gtcggcgagc gccgcttcat cgtgccgtcg ctcgtgttcc
     4861 tgctgctgac ggtcggcagt ttcgcgctgt attccaggct gctcgaccag gaactcgagt
     4921 tcgtgcgccg ccaggtacag aacgattccg ctgaaatcga ttcgcagctg cgcgtgcgca
     4981 cggccgagca catcgcgaca cttgcccgca tggcggcgcg ctggaagtcg gccggcggca
     5041 cgaatttcga cctctggcgc agcgacgcca ccaacctgct caacgcgtat cccgggctgc
     5101 gcgcgctgca gtggatcgac tccacccatc acgtccgctg ggtcgaaccg ctggcaggca
     5161 acgaaggcga gctcgagcgt aacgtactgg gcggcgatat ccgcgagggc gcgctgcgcg
     5221 ccgcggcaga gaccagccgc gagctgctga ccgcgcccat gcagacgcgg cagggctacg
     5281 cgggtttcac tgcctatcta ccggtacggc gggaaggccg tcccgatgga ttcatcgccg
     5341 gacttttctc ggtacccaac ctcttcaatg gcgccgtcga gacccggctg tcaggtgact
     5401 acgcgatttc aattcagttt ggtggggcaa cggtctacgc caacagccgc ggcgacagcc
     5461 cgctgacgcg tggcctggca accgaaaagg acctgcgaat cggaggtcgt acgtggaagc
     5521 tgcgcatgac gcccactccc gatttcgtgg agcggcagaa atccggactg ccgctgctgg
     5581 tgctcggcgc tggcctgctg gtggcggcgc tggcggccct ctcggtacgt ttcgtgctca
     5641 tctcccgcct caagtctgcg catctcgcaa agtcgctggc actcaacgcg ggaatcattt
     5701 cgagcagcgc ccacctggtc atcgcgatcg ac
//
LOCUS       DASQJS010000206         3151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_426337, whole genome shotgun sequence.
ACCESSION   DASQJS010000206 DASQJS010000000
VERSION     DASQJS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426337"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VEW08_08415"
     CDS             <1..1165
                     /locus_tag="VEW08_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ40798.1"
                     /translation="DKTGAQRLELNLGGRSSDYETAGRVETYKADGLWEPINGFTFRG
                     GFEHAIRAPNIGELYNQAGAQAQIGSPPGQGDPCDTRSTARTGANGASVRTLCGQTGV
                     PGAILDTYQYTTVAIGVINSGNTDLTPEEADTLTFGFVWSPEFDKPLFADLSFSVDWY
                     DIDISNVIAPIAGGTALSKCYNLDGTNPTYDAANPFCAALRRNPTTGGVDTVAAPYLN
                     LGGLHTTGVDAQIDWKIPAGPGSLDLNILANFTDSFETQLLVGSAWQEFAGTIDGTQN
                     GGIPIPDWKTLTSLTYRLPNFEGGVRWRHLPEMKDVTSVTRPASPAPGVPSYDLFDIT
                     MVYKFNEKILVRGGVNNVADEEPPIVGGTIGQTQPGTYDILGRYYYLGLQLGF"
     gene            1188..1574
                     /locus_tag="VEW08_08420"
     CDS             1188..1574
                     /locus_tag="VEW08_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ40799.1"
                     /translation="MTIESIHHVNIRAPATDIAKLRQFYCEIVGLRDGWRPPFESRGH
                     WLYAGPHPVVHLVEGGDDTDKASGGGVDHVAFRCSDLAPMIERLRSRGIDFQLTQVPS
                     LGDQQLLFRDPLGIGVELTVAGAARC"
     gene            1568..>3151
                     /locus_tag="VEW08_08425"
     CDS             1568..>3151
                     /locus_tag="VEW08_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HYJ40800.1"
                     /translation="MLSCIATISVNGPLRTKLRAIADAGFEHVEIFESDLLASPEPVH
                     VIGAMMRDLGLTCVAFQPFRDFEGMPAAERARVFDRAERKFDVLQELGTDLMLVSSNV
                     SPAALADRGRIVDDFRELGERAAARGLRVAFEALAWGRHVRDHRDAWDIVRQADHAAV
                     GLALDAFSSLAPGIPIDSLGAIRREKLFHVQIADAPKLTMDPLSWSRHFRCMPGQGDL
                     PLVDYVGALRRIGYDGVLSLEIFNDRFRAGSTTEVALDGMRSLAYLLEQVDRKPDPRT
                     VCKGIEFIEFCANEEEASQLSQMLGMMGFAATHRHVRKKVTRWRQGDINVVVNCEPEG
                     FAHSYDTVHGASVCAIGLRVADPDAALRRAVQLQIPSFSQPVGPGEYEIPALRGVGGS
                     LLYFMKESDTPAVWKTEFEALPTDPSRVDAGLTRIDHFSQSMQFEEMLSWLLYYVTLF
                     AVEKSAAVEIADPVGLVQSQAVQSGDRSVRILLNSSAAGQTLVDRFLHAYGGAGVQQV
                     SFATRDIFTTAARLRALGME"
BASE COUNT          554 a          997 c         1022 g          578 t
ORIGIN      
        1 cgacaagacc ggtgcacagc gcctggaact caacctgggt ggcagaagct ccgactacga
       61 aaccgcaggc cgcgtcgaga cttataaggc cgacggcctg tgggagccga tcaatgggtt
      121 cacattccgc ggtggtttcg aacatgccat ccgggcgccc aacatcggcg aactctacaa
      181 tcaggccggt gcgcaggcac agatcggatc accgcccggc cagggcgatc cctgcgacac
      241 gcgttccacg gcgagaaccg gtgcgaatgg cgccagcgtt cgtacgcttt gcgggcagac
      301 gggcgttccc ggtgcgatcc tggataccta ccagtacacc acggtcgcga tcggcgtcat
      361 caacagtgga aacacggatc tgactcccga agaagccgac acgctgacct tcgggttcgt
      421 gtggtcgccc gagttcgaca agccactgtt cgcggacctg tcgttctcgg tggactggta
      481 cgacatcgac atcagcaatg tcatcgcgcc gattgccggt ggcactgcgt tgagcaagtg
      541 ctacaacctc gacggcacca acccgaccta cgatgcggcg aacccgttct gcgcggccct
      601 gcggcgcaat ccgacgacgg gtggtgtcga cacggtcgcc gcgccgtatc tgaacctggg
      661 cggattgcac acgaccggcg tggacgcgca gatcgactgg aagattcctg ccggtcccgg
      721 cagcctcgac ctcaacatcc tggccaactt caccgactcg ttcgagacgc agttgctcgt
      781 cggttcggcc tggcaggagt ttgcgggcac gatcgacggc acgcagaacg gcggcattcc
      841 gattccggac tggaagacgc tgacgtcgct gacgtaccgg ctgccgaatt tcgaaggcgg
      901 cgtgcgctgg cgccatctgc cggagatgaa ggacgtcacc tcggtcacgc ggcccgcgag
      961 cccggcgccg ggggtgcctt cgtatgacct cttcgacatc acgatggtct acaaattcaa
     1021 cgagaagatc ctggtccgcg gcggtgtcaa caacgtggcc gacgaagagc cgccgatcgt
     1081 cggtggcacg atcggccaga cgcagccggg cacgtacgac attctcggcc gctactacta
     1141 cctgggcctg cagctgggtt tctaactatc aattcgacaa gtacaaggtg acgatcgagt
     1201 caattcatca tgtgaacatt cgggctcccg caacggacat cgcgaagctc cggcagttct
     1261 actgcgagat cgtcgggctt cgcgatggct ggcgcccacc gttcgaatcg cgcggccact
     1321 ggttgtatgc gggaccgcac cccgtcgtgc acctcgtgga aggcggggat gataccgaca
     1381 aggcatcggg aggcggcgta gatcacgtcg ccttccggtg ctccgatctc gcgccgatga
     1441 tcgagcggct ccgaagcagg ggaatcgatt ttcagctgac ccaggtgccc tccctcggcg
     1501 atcagcaact gctgtttcgg gatccgctcg gcatcggcgt cgagctcacc gttgcgggag
     1561 ccgccaggtg ctgagctgca tcgccaccat ttccgtcaac ggcccgctgc gcaccaagct
     1621 gcgcgcgatc gccgatgccg gcttcgagca cgtggagatc ttcgagagcg acctgctggc
     1681 ttcgcccgaa ccggtgcacg tcatcggcgc gatgatgcgc gacctggggc tcacctgcgt
     1741 ggcgttccag ccattcaggg acttcgaagg aatgccagcg gccgaacgtg cgcgcgtgtt
     1801 cgatcgcgcc gagcgcaagt tcgacgtact gcaggagctc ggcacggacc tgatgctggt
     1861 gagctccaat gtctcaccgg ctgcgctcgc cgatcgcggc cggatcgtcg atgatttccg
     1921 ggagctcggc gagcgtgctg ccgcgcgcgg gttgcgcgtg gcattcgagg cgctggcctg
     1981 gggccggcac gttcgtgatc accgcgacgc gtgggacatc gttcggcagg ccgatcacgc
     2041 tgcggtcggg ctggcgctcg atgccttcag ttcgctcgcg cccggcattc cgatcgacag
     2101 tctgggcgcg atccggcgcg aaaagctgtt ccatgtgcag attgccgatg cgccgaaact
     2161 cacgatggat ccgctgtcgt ggagccgcca tttccgttgc atgcccgggc agggcgacct
     2221 gccgctcgtg gactacgtgg gcgcgctgcg gcgaatcggt tacgacggcg tgctgtcgct
     2281 cgagatattc aacgatcgct tccgcgccgg ctcgaccacc gaagtcgcgc tcgacggcat
     2341 gcgctcgctg gcctatctgc tcgagcaggt ggaccgaaag ccggatcctc gcaccgtctg
     2401 caagggaatc gagttcatcg agttctgcgc gaacgaggaa gaggcgtcgc aactcagcca
     2461 gatgctcggc atgatgggct ttgccgccac gcaccggcac gttcgcaaga aggtcacgcg
     2521 ctggcgccag ggcgacatca acgtagtcgt caattgcgag cccgaaggtt ttgcacattc
     2581 ctatgacacc gtgcatggag cgtcggtgtg cgcgatcgga ttgcgcgtgg ccgatccgga
     2641 cgcggcgctg cgccgtgcag tgcagctgca gattccgagc ttttcgcaac ccgtgggccc
     2701 gggcgagtac gagatcccgg cgttgcgcgg tgtcggtggc agcctgctct atttcatgaa
     2761 ggaatccgat acgccggcgg tctggaagac cgagttcgag gcgcttccga cggatccctc
     2821 gcgcgtcgat gcgggcctta cgcgcatcga ccatttctcg cagagcatgc aattcgaaga
     2881 gatgctttcc tggctgctgt actacgtgac tttgtttgcg gtcgaaaaaa gcgcggcagt
     2941 ggagattgcg gacccggtgg gcctcgtgca gagccaggcg gtgcagtcgg gcgaccgcag
     3001 cgttcgcata ctcctgaaca gctcggcggc agggcagact ctggtcgacc gtttcctgca
     3061 tgcctatggc ggcgcgggtg tccagcaggt ttcatttgcc acgcgcgaca tctttaccac
     3121 cgccgcgcgc ctgcgtgctc tcggcatgga a
//
LOCUS       DASQJS010000207         9958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_426519, whole genome shotgun sequence.
ACCESSION   DASQJS010000207 DASQJS010000000
VERSION     DASQJS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9958
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426519"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VEW08_08430"
     CDS             <1..549
                     /locus_tag="VEW08_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HYJ40801.1"
                     /translation="LSGGNFHAEPVAFAADNLALAVAEIGALAERRIALLIDATLSGL
                     PPFLVRDGGVNSGFMIAHVTAAALASENKLLAHPASIDSLPTSANQEDHVSMATFAAR
                     KLGDLAENTAIILAIELLAAAQGVDLRAPHLTSEKLQRVMREIRARVPHYDIDHYLAP
                     DIQAMADAVNGGVIAAHAPIPA"
     gene            546..1751
                     /locus_tag="VEW08_08435"
     CDS             546..1751
                     /locus_tag="VEW08_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HYJ40802.1"
                     /translation="MSIPVEFVLFGLTLLGVAVFHHHTLKVALLGLAAIIGYKLFFGD
                     FYGQPGLLGLWFHFLEEWVVLANLFALLVGFALLSRHFEESHVPHLMPRFLPDDWKGG
                     FVLLAMVFVLSAFLDNIAAAIIGATVAGTIYRHRLHIGFLAAIVAASNAGGAGSVVGD
                     TTTTMMWLEGVAPLDVVHAYVASVAAFFVFAIPAARAQQRHSPIEKDPDPQIHLDWAR
                     VIIVVLILVCAIAANIHANSLPPEQSGAFPYIGATVIGVILLVTPWRRPDWKAVPAAA
                     TGSVFLLSLVLAASMMPVEKLPEASIATAFGLGFVSALFDNIPLTKLALEQGGYDWGV
                     LAYAVGFGGSMIWFGSSAGVAVSNLFPEAKSVGQWLRHGWYVAVGYVVGFIALIAVLG
                     WNPRDIGHG"
     gene            complement(2177..3604)
                     /locus_tag="VEW08_08440"
     CDS             complement(2177..3604)
                     /locus_tag="VEW08_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYJ40803.1"
                     /translation="MHSVARSVRKTPGPLWVNDTHSALNHTAVSRLVEPCWVGEVAEA
                     VARARRQREPLAISGSRHAMGGQQFLTGGTLLDMRHLNHVRWFDRGRALMEVEAGITW
                     PDLIRGYLALQRGPGTAFGIRQKQTGADRLTLGGAVSANIHGRGLRAQPFVADVEGLE
                     VVTPTGEILRCSRTEHAELFRHVVGGYGLFGVVTAVTLRLVPRVKVERCVARLEIDSL
                     IDAFDERIAAGHLYGDFQFATAPDSPEFLRTGVLSSYRPVAGDREIPKDQRRLSQSDW
                     NQLLTLAHRDKRAAFEKFSEFYLATHGQLYWSDTHQLNLYLEDYHGALDHRLGSAVRG
                     SEMITELYVPRPALLAFMNDVRRDFRLNGVDFIYGTIRLIEAENETALPWARESYACV
                     IFNLHIDHRREDVARVADHFRRLIDLSLRHGGSYFLTYHRFASASQVEAAHPAIRQFL
                     ATKRRLDPEGVFQSDWYRHLAGLFE"
     gene            3722..4459
                     /locus_tag="VEW08_08445"
     CDS             3722..4459
                     /locus_tag="VEW08_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYJ40804.1"
                     /translation="MGDTTRIILALKRALRSRGITYAMLAKRISLSEPSVKRILSRGS
                     LSLPRLERICEASGISLEELIRPVSSGPAETSSTLSAVQETALAADPQLLACFYLMTN
                     GHTPREVATQLGADEKQLRRWLVKLDNLHLVELRARLGARLRVHAPISWRDDGPIRRT
                     YEQQVREEFMRSSFLAPDEVLQFRSAEMSEASRVVLQRKLARLTADFNELAELDRHLP
                     SREKRSVALMTACRPWVWSMFDGLRRR"
     gene            complement(4401..4871)
                     /locus_tag="VEW08_08450"
     CDS             complement(4401..4871)
                     /locus_tag="VEW08_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40805.1"
                     /translation="MLETYQTKLTDVLVWTAGAAFAASWFLPVLVDVPGWMAFRYALA
                     PLVPYSDAGNLTWDDSIPQVLSALTNLVFLILFTLWVTRQKVRPGLFVRITLACFLLN
                     LYWFVKAWRDDGLGDLLVGYYAWQAAFALLLGVAVIIVVANRRTSTTPTAGKPS"
     gene            complement(4887..5375)
                     /locus_tag="VEW08_08455"
     CDS             complement(4887..5375)
                     /locus_tag="VEW08_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40806.1"
                     /translation="MKKSLILSLAVLALAACETPNGAGTGSGNVRGTQKPVAFTWESD
                     DSNSGDITATFGSGRVFTGEYVQISRDISEKNLDPLWDGWDRPRSKNDWRYWNADSRS
                     EFVRENSGRVLANLHDEKGEYMRCRFRLANPERGMKGGGEGRCQLSDSGKEINAEFER
                     QS"
     gene            complement(5439..6047)
                     /locus_tag="VEW08_08460"
     CDS             complement(5439..6047)
                     /locus_tag="VEW08_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYJ40807.1"
                     /translation="MNVPPAYHEGMRELQDLRETRALADRLEKVTMRAAFTEEDKAFI
                     QRCRMFFLATADAQGQPDCSYKGGLPGFVRVLDEHTLAIPDYDGNGQYRSWGNLRVNA
                     QVGLLFVDFETPKRLRVNGTAVVSQDDPLLAEFPGAVFLVRMTAQRIFPNCPRYIHKM
                     QLVEESVYAPRPQYTPPVPAWKTFDVFKDALPPRDRPTEAAD"
     gene            complement(6052..6747)
                     /locus_tag="VEW08_08465"
     CDS             complement(6052..6747)
                     /locus_tag="VEW08_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HYJ40808.1"
                     /translation="MFELNKDDIEMIVRLLAALGAGALIGYERSFHGRPAGLRTHVLV
                     CLASALLMLVTVYEDHWVRSQGESRLDPTRMAQGIMTGIGFLGAGVIVKEGLNVRGLT
                     TAASIWITAAIGVLAGVGLYLPMAVSTILTLSVLSLFRWIEMRVPTQAYYNFDVKYEN
                     DGNLSEEEMRGLIQRHKFSIANFSYRLDAEGRVLRHSMTLQTTDRGAASRLAHTLKEN
                     STVIEFRISPTGD"
     gene            complement(6783..7514)
                     /locus_tag="VEW08_08470"
     CDS             complement(6783..7514)
                     /locus_tag="VEW08_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40809.1"
                     /translation="MPAADRITTTVHATADVTPAQWNDIWTLTEEFYDVERAYAEAEL
                     RQRQTIAMFHMNDSLLGMAAIDVYPTEFRGQKLVVIATHHVLIREAWRGRNLLQKLGA
                     RTFLATRLRYPLRPIYWFFDTFSYKSYLLLPRNFRQFWPRHEEPTPEPSAALIDELAT
                     RTYGPAWRPERGVVVRSGQKRMRSTSAPLVLGTGADPALEFFARVNPGHPDGDMLVCL
                     CPLTLSNWLSLARKALRRLERPGMH"
     gene            7605..8063
                     /locus_tag="VEW08_08475"
     CDS             7605..8063
                     /locus_tag="VEW08_08475"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYJ40810.1"
                     /translation="MAPVHASMLIHRPPGEVFAAFVNPQQLRKFWLSDASGPLAPRAR
                     VQWSFMVPGATARVVVTRFESPHHLAFDWDDEMHVAMTFEPFGDGGTRAGVEVSGFTG
                     ADAVQQATNTIEGFAIVLCDLKTLLETGESPGLVRAKAELIIAGQAAQGG"
     gene            8190..>9958
                     /locus_tag="VEW08_08480"
     CDS             8190..>9958
                     /locus_tag="VEW08_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYJ40811.1"
                     /translation="MPLPQFHPAVQAWFASTFPGGATPAQLQAWPAIASGQHALIAAP
                     TGSGKTLAAFLAAIDSLAREGAEGRLRDVTRVVYVSPLKALSNDIQRNLEQPLAGVSS
                     EMLKLGSCAPEIRAAVRTGDTTQSERAAMRRTAPHILVTTPESLYLLLTSASGREMLS
                     TVRTVIVDEIHAVAQTKRGAHLAITLARLAANAAQPIQRIGLSATQKPIEEVAQFLVG
                     GELVPGGESGECGRSGLSLALPFGPHSPLSPPGTNVGKTIIVNSGHVRDRDINLVMPA
                     APLEAVMSGEVWATVYDQIAKLIEEHHTTLVFANTRRMVERVSRHLAERLGEGNVAAH
                     HGSLSKETRFDAEQRLKAGKLKVMVATASLELGIDIGDVELVCQLGTPRSISVFLQRV
                     GRANHSVAGVPKGRIFPSSRDELIDCVALLDSVHRGELDRLRIPEHPLDVLSQQIVAE
                     VSAREYGEDDLFDLMRSAHPYRNLDRKDFDAVVRMLAEGIDTKRGRRGTYLHRDAVNK
                     RLRPRKGARLTAITCGGAIPDNADYQVILEPAGIFVGTLNEDFASESLAGDIFQLGNT
                     SYKILRVEAGRVRVEDAKGQPPS"
BASE COUNT         1719 a         3256 c         3244 g         1739 t
ORIGIN      
        1 ctttccggcg ggaatttcca cgccgagccg gtggcgttcg cggcggacaa cctggcgctc
       61 gccgtggccg agatcggcgc gcttgccgag cgccgcatcg cgctgctcat cgacgccacg
      121 ctctcgggct tgccgccgtt cctcgtgcgc gatggcggcg tgaattcggg attcatgatt
      181 gcgcacgtca cggccgcggc gctcgcgtcc gagaacaagc tgctcgcgca cccggccagc
      241 atcgattcgc tgcccacctc cgccaaccag gaagaccacg tctccatggc caccttcgcg
      301 gcgcgcaagc tcggcgatct cgctgaaaac acggcgatca ttctggccat cgaactgctg
      361 gccgcggcgc agggcgtgga cctgcgcgcg ccgcatctca cctcggagaa actgcagcgt
      421 gtgatgcgcg agatccgcgc gcgcgtgccg cactacgaca tcgaccacta cctcgcgccg
      481 gacatccagg ccatggcgga cgccgtcaat ggcggcgtga tcgcggccca cgcaccaata
      541 cctgcgtgag cattccggtc gagttcgtgc tcttcgggct gacgctgctc ggcgtggccg
      601 tgtttcacca ccacacgctg aaggtggcgc tgctgggcct ggcggcgatc atcggctaca
      661 agctcttctt cggcgacttc tacggccagc ccggactgct cggactttgg tttcacttcc
      721 tcgaagagtg ggtggtgctc gccaacctgt tcgcgctgct ggtgggtttt gcgctgttgt
      781 cccggcactt cgaagagagc cacgtgccac acctgatgcc gcggttcctg cccgacgact
      841 ggaagggtgg ttttgtactg ctggcgatgg tgttcgtgct ctcggcattt ctcgacaaca
      901 tcgccgcggc tatcattggg gccacggtgg cgggcactat ctaccgtcac aggctgcaca
      961 tcggttttct cgcggccatc gttgctgcct ccaacgcggg cggcgccggc agcgtggtgg
     1021 gagacaccac caccaccatg atgtggctgg agggcgtggc cccgctcgac gtggtgcatg
     1081 cctacgtcgc ctcggtggcg gcgttcttcg tgttcgcgat tccggcggcg cgcgcgcagc
     1141 agcgtcattc gcccatcgag aaagatcccg acccgcagat tcacctcgac tgggcacgcg
     1201 tcatcatcgt cgtgctgatt ctcgtctgcg ccatcgccgc caatattcac gcgaacagtc
     1261 tgccgcccga gcagtcgggc gcattcccgt acatcggcgc aaccgtgatt ggcgtgatcc
     1321 tcttggtcac tccctggcgg cggccggact ggaaggccgt gccggcggca gcgaccggca
     1381 gcgtgttcct gctctcgctg gtgttggcgg cgtcgatgat gccggtggaa aagttgcccg
     1441 aggcttccat cgccacggct ttcggcctcg ggttcgtttc ggccttgttc gacaacatcc
     1501 cgctcacgaa actcgcgctc gagcagggtg gctatgactg gggcgtgctc gcttatgcgg
     1561 tgggctttgg cggctcgatg atctggttcg gatcgtcagc cggcgtcgcc gtttccaact
     1621 tgtttcccga agccaaatcc gtaggccagt ggcttcggca tggctggtat gtcgccgtag
     1681 ggtatgtcgt cggcttcatc gcgctgatcg cggtgctggg ctggaatccg cgggacatcg
     1741 ggcacggctg aagtccggcc gctcaccagc acgaacagcg ccagcaccca gtggaaggcg
     1801 accggaattg caccctcgcg ccagaagtcc gcaccgttcg cattgacgtt cacggcgagg
     1861 cgcactgcca tcaccaggaa atagatcccc gccaggatgc gtagcgcctc cgccacccgc
     1921 ggacgcgaac gcgcgaagct gccgttgcgc actcgccggt tccaggccac caccgccatc
     1981 agcatgagaa tgccgatctg aaccggcagc aggaccgggt agggaagcag gcccgagtac
     2041 caggcctcca tgtccggcaa ccagtctggc gctactaaca aggcctggac ctggccgatt
     2101 acacgcgcgg cgaacagggc aacgcaactc cacagcagcc aggcggcaga ttccgtatgc
     2161 gatgccgcgc cgcggatcat tcgaacagac ccgccaggtg tcgataccaa tcgctctgga
     2221 aaaccccttc aggatcgagc cttcgcttgg tcgccaggaa ctgccggatc gcgggatgcg
     2281 cggcttcgac ctgggaggcg ctggcgaagc ggtggtacgt gaggaagtag ctgccaccat
     2341 ggcgcagcga gagatcgatg agccggcgaa agtgatcggc gacgcgcgcc acgtcctcac
     2401 ggcggtgatc gatgtgcaga ttgaagatca cgcaggcgta actttcgcgc gcccagggca
     2461 acgcggtttc gttttccgct tcgatgaggc gaatggtgcc gtagatgaag tcgacgccat
     2521 tcagccggaa atcgcgccgc acgtcgttca tgaatgcgag cagggcgggg cgcggcacgt
     2581 agagctcggt gatcatctcg ctgccgcgca cggcggagcc gaggcggtga tcgagcgcac
     2641 cgtggtagtc ctcgaggtac aggttgagct ggtgcgtatc gctccagtac agctggccgt
     2701 gggtcgccag gtagaactcc gagaactttt cgaaggcggc gcgtttgtcc cggtgcgcga
     2761 gtgtcagcag ctggttccag tcactttgcg acaggcgtcg ctggtcctta ggaatttcgc
     2821 gatcgccggc cacaggccgg tagctcgaca gaaccccggt gcgcagaaac tccgggctgt
     2881 cgggcgcagt cgcgaactgg aagtcgccat acagatgacc cgctgcgatg cgttcatcga
     2941 atgcgtcgat gagggaatct atttccaggc gtgcgacgca acgctccact ttcacgcgcg
     3001 gcaccagccg cagcgtcacc gccgtgacga cgccaaacaa tccgtaaccg cccaccacgt
     3061 ggcgaaacag ctcggcgtgt tccgtgcgac tgcagcgcag gatttcgccg gtaggcgtga
     3121 cgacttcgag accttcgacg tccgcgacga acggctgggc acgcaggccg cggccgtgaa
     3181 tgttcgcgga gaccgcgccg cccagcgtga ggcggtcggc accggtctgt ttctggcgga
     3241 taccgaatgc cgtgcccggg ccgcgctgca atgccaggta cccgcgaatc agatccggcc
     3301 aggtgatgcc agcctccact tccatgagcg cacggccgcg gtcgaaccag cgcacgtgat
     3361 tgaggtgccg catgtcgagc agcgtgccgc cggtgagaaa ctgctggcca cccatggcat
     3421 gacgcgaacc gctgatggcg agtggctccc gctggcgacg cgctcgcgcc acggcttccg
     3481 cgacctcacc cacccagcag ggctccacga ggcgactgac cgcggtgtgg ttgagcgccg
     3541 aatgggtgtc gttgacccag agcgggcctg gcgtcttgcg aaccgaacga gcgacggagt
     3601 gcatgggagc cgaagctggc tcaaccccgg tgcccagggc aataccccaa aaccgggtat
     3661 cggatagcga tactgcgggt gccggcgcca gaaggcggaa tttccggcag aatcggccgc
     3721 catgggagac acaactcgaa tcatcctcgc gctgaagcgc gcgctgcgtt cgcgcggcat
     3781 cacctacgcc atgctcgcca agcgcatctc gctgagcgag ccctcggtca agcgaatcct
     3841 gtcgcgcggc tcgctgtcat tgccgaggct ggagcgcatc tgtgaagcct cgggcatttc
     3901 cctggaagag ctgatccggc cggtgtcgtc ggggccggcg gagacgtcca gcaccctgag
     3961 cgccgtgcag gaaaccgcgc tggccgcgga tccgcagttg ctggcctgct tctatctcat
     4021 gaccaatggg cacacgccgc gcgaggtcgc cacgcagctc ggcgcggatg agaagcagct
     4081 gcgccgctgg ctggtaaaac tcgacaacct gcacctggtg gaactgcggg cgcgcctggg
     4141 ggctcgcctg cgcgtgcatg cgcccatttc ctggcgagac gacggcccca tacgccgcac
     4201 ctacgaacaa caggtccgcg aggagttcat gcgatcgagc tttctcgcgc cggatgaagt
     4261 gctgcaattc cgttcggcgg aaatgtcaga ggcctcgcgc gtggtcctgc agcgcaagct
     4321 ggcgcgtctg actgcggatt tcaatgagct cgcggagctc gatcgtcacc tgcccagccg
     4381 cgagaagcgc agcgtggcgc tcatgacggc ttgccggccg tgggtgtggt cgatgttcga
     4441 cggtttgcga cgacgatgat cacggccacg ccgagcagca gggcaaacgc cgcctgccat
     4501 gcgtagtagc cgaccagcag gtcgccgagg ccatcatcgc gccaggcttt tacgaaccag
     4561 taaaggttca gcaggaagca ggcgagcgtg atgcgcacga acaagccggg ccgcaccttc
     4621 tgtctggtga cccacagcgt gaacaggatc aggaacacca ggttggtgag cgcgctcagg
     4681 acctggggta ttgaatcgtc ccaggtgaga ttgcccgcgt cgctgtacgg caccagcggc
     4741 gccagcgcat aacgaaacgc catccatccg ggcacgtcca ccagaacggg caagaaccac
     4801 gaggcggcaa atgccgcccc cgcggtccat accaggacgt cagtcagctt cgtttgatag
     4861 gtctcgagca ctcctcgcgg agcgtatcaa gactggcgtt cgaactccgc gttgatttcc
     4921 ttgcccgagt ccgacagctg gcatcggcct tcaccgccgc ctttcatgcc gcgctcgggg
     4981 ttggccaacc ggaatcggca gcgcatgtat tcgcctttct cgtcgtgcag attggcgagc
     5041 acgcgaccac tgttttcccg cacgaactcg gaacgcgaat ccgcgttcca ataacgccag
     5101 tcattcttgc ttcgcgggcg gtcccagccg tcccagagcg ggtccaggtt cttctcgctg
     5161 atgtcgcgcg agatctgcac atattcaccg gtgaacacgc ggccggagcc gaacgtggcg
     5221 gtgatgtctc cggaattgct gtcgtcgctt tcccaggtga atgccaccgg tttctgcgtg
     5281 ccgcgcacgt tgccagagcc ggttcctgcg ccgtttggag tttcgcaagc ggccagcgcg
     5341 agcacggcaa gcgacaagat gagtgacttt ttcatgccgc aatcctggcg cgcttccgtg
     5401 cgcgggaatg caggacaaac aacacaagcc ttgtaaggtt agtcggccgc ttccgtagga
     5461 cggtcgcgcg gcggcagtgc gtccttgaag acgtcgaagg ttttccacgc gggcaccggc
     5521 ggtgtgtatt gcggccgcgg agcgtacacc gactcttcca cgagctgcat cttgtggatg
     5581 tagcggggac agttgggaaa gatgcgctgc gccgtcatgc ggaccaggaa caccgccccc
     5641 gggaattccg ccagcagcgg gtcgtcctgg ctaactaccg ccgtgccgtt cacgcgcagg
     5701 cgtttcggag tctcgaagtc cacgaacagc agccccacct gcgcgttgac gcgcaagttg
     5761 ccccagctgc ggtactggcc gttgccgtca tagtcgggaa tcgccagtgt gtgttcgtcg
     5821 agcacgcgta cgaatcccgg aagcccgccc ttgtacgagc agtcgggctg gccctgcgcg
     5881 tcggctgtgg cgaggaaaaa cattcggcag cgctggatga atgccttgtc ttcctcggtg
     5941 aacgcggcgc gcatcgtcac tttttccagt cgatcggcaa gggcgcgcgt ctcgcgcagg
     6001 tcctgcagtt cgcgcatgcc ttcgtgatag gccggtggca cgttcatggc ctcagtcccc
     6061 cgtcggtgaa atccggaatt cgatcaccgt cgaattttcc ttgagcgtat gcgcgagcct
     6121 cgaggccgcg ccgcgatccg tggtctgcag cgtcatgctg tgccggagga cgcgtccttc
     6181 ggcgtccagc cgatagctga agttggcgat gctgaacttg tggcgctgga tgagcccgcg
     6241 catttcctct tccgagaggt tgccgtcgtt ctcgtatttc acgtcgaagt tgtagtacgc
     6301 ctgcgtgggc acgcgcattt cgatccagcg gaacagcgac aataccgaga gcgtgagaat
     6361 ggtggacacg gccattggca ggtacagacc gactcccgcc agcacgccga tggccgcggt
     6421 aatccagatg gaggcggcag tagtgaggcc gcgcacgttc aggccttcct tgacgatgac
     6481 gccggcgccg agaaagccga tgccggtcat gatgccctgg gccatgcgcg tggggtcgag
     6541 tcgcgattcg ccctggctgc gcacccaatg gtcctcgtac accgtcacca gcatcagcag
     6601 cgcggaggcc aggcagacga gcacatgcgt gcgcagcccg gcgggtcggc cgtggaacga
     6661 gcgctcgtag ccgatgagcg cgccggcacc gagcgcggcc agcagccgca cgatcatctc
     6721 gatgtcgtct ttgttgagtt cgaacatggg cagggggaga gtcgcgccag acgcgattgc
     6781 cgtcaatgca tcccgggccg ctccagccgc cgcagcgcct tgcgggcgag gctgagccag
     6841 ttcgacaggg tgagcgggca caggcagacc agcatgtcgc cgtccgggtg gcccggattg
     6901 acgcgcgcga agaattccag cgccggatcg gcacccgttc ccagcaccag cggcgcggag
     6961 gtggaccgca tgcgtttctg tccggacctc accacgactc cgcgctccgg acgccacgcc
     7021 ggcccgtacg tccgggtcgc cagttcgtcg atgagtgccg cactgggttc cggcgtcggt
     7081 tcctcgtgcc gcggccagaa ctggcggaaa ttgcgcggca agagcaggta gctcttgtag
     7141 ctgaaggtgt cgaagaacca atagataggt cgcagcgggt accgcaggcg cgtggcgagg
     7201 aaggtgcggg ccccgagctt ctgcagcaga ttgcggccgc gccatgcctc gcggatcagc
     7261 acatgatggg ttgcgatcac gaccagcttc tggccacgga attcggtcgg gtacacatcg
     7321 atggccgcca tgccgagcag cgaatcgttc atgtggaaca tcgcgatggt ctggcgctgg
     7381 cgaagttcgg cctcggcgta cgcccgttcc acgtcgtaga actcttcggt caacgtccag
     7441 atgtcgttcc attgcgcagg cgtcacgtcg gcggtggcgt gcacggtggt agttattcgg
     7501 tctgccgcgg gcatgtctga gcttacgtcg gacgcaaggt ggccagcaaa ggcgatccac
     7561 ctactgcgca acctgaggcc gcgtgatcca atgccggcat ggacatggca cccgtacatg
     7621 cgtcgatgct catccaccgt ccacccgggg aagtcttcgc cgccttcgtg aatccgcagc
     7681 agctacgcaa gttctggctg agcgacgcca gcggtccgct cgcgccgcgc gcgcgtgttc
     7741 aatggagctt catggtgccg ggcgccacgg cccgggtcgt ggtcacgcgg tttgaatcgc
     7801 cccaccacct ggcgttcgac tgggatgatg aaatgcatgt ggccatgacc ttcgagccgt
     7861 tcggcgacgg cggcacgcgc gccggagtcg aagtcagcgg ctttaccggc gcggatgccg
     7921 tgcagcaggc gaccaacacg atagagggct tcgccatcgt gctctgtgac ctcaagacgc
     7981 tgctcgaaac gggtgaatcg cccggcctgg tgcgcgccaa ggccgagctc atcatcgcgg
     8041 gccaggctgc gcagggcggc tgagtcgtat ctggcggatt tcgctggatt tccgacgcgc
     8101 tcccggcgat aggaaagggc gatatacagg ggtgcaggcg gggggatgct ggatggatcg
     8161 tcccggcgcc tctatataaa gtgccaccca tgccactccc ccagtttcac cccgccgtgc
     8221 aggcgtggtt cgccagcact tttcccggcg gcgcaacgcc ggcgcagctt caggcctggc
     8281 ccgcgatcgc gagcggccag cacgcgctga tcgcggcgcc gaccggctcg ggaaagacgc
     8341 tggccgcgtt tctcgcggcc atcgattcgc tggcgcgcga gggggcggaa gggcgcctgc
     8401 gcgacgtaac gcgcgtcgtg tacgtgtcgc cgctgaaggc gctttcgaac gacatccagc
     8461 gcaatctcga gcagccgctc gcgggagtct cctccgagat gctgaagctc ggctcctgcg
     8521 cgccggaaat ccgcgccgcc gtgcgcaccg gcgacaccac gcagagcgag cgcgccgcca
     8581 tgcgccggac cgcgccccac attctcgtga ccacgcccga gtcgctctac ctactgctga
     8641 ccagcgcctc gggccgcgaa atgctgtcca ctgtgcgcac cgtcatcgtc gacgaaatcc
     8701 acgccgtggc gcagaccaag cgcggcgcgc atctcgcgat tacgctcgcg cgcctcgccg
     8761 ccaatgccgc gcaacccatc cagcgcatcg gcctgtccgc aacgcagaag cccatcgagg
     8821 aggtcgccca gttcctcgtg ggtggggagt tggtgccggg tggggaaagt ggggagtgcg
     8881 gccggagcgg cctgagtctc gcactaccgt tcgggccgca ctccccactt tccccacccg
     8941 gcaccaacgt cgggaaaacc atcatcgtca actccggcca tgtacgtgat cgcgacatca
     9001 acctggtgat gccggcggcg ccgctcgagg cggtgatgtc gggcgaagtg tgggccacgg
     9061 tctacgacca gatcgcgaag ctcatcgaag agcaccacac gacgctggtg ttcgcgaaca
     9121 cgcgccgcat ggtcgagcgc gtctcgcggc acctggcaga gcggctcggc gaaggaaacg
     9181 tggcggccca ccatggcagc ctctccaagg aaacccgatt cgacgccgag cagcgcctca
     9241 aggcaggcaa gctcaaggtg atggtggcca ctgcctcgct cgagctgggc atcgacatcg
     9301 gtgacgtgga gctggtgtgc cagctcggca cgccgcgcag catctcggta ttcctgcagc
     9361 gcgtgggccg cgccaatcac tccgtcgccg gcgtcccgaa gggccgcatc ttccccagca
     9421 gccgcgacga gctcatcgac tgcgtcgcac tgctcgattc cgtgcatcgc ggcgagctcg
     9481 accgcctgcg cattcccgaa catcccctcg acgtgctctc gcagcagatc gtcgcggaag
     9541 tgtccgcgcg cgagtacggc gaggacgacc tgttcgacct catgcgcagc gcgcatccct
     9601 atcgcaatct cgaccggaaa gatttcgacg ccgtggtgcg catgctcgcc gagggcatcg
     9661 acaccaagcg tggccgccgc ggaacctatc tacaccgcga tgcggtgaac aagcgtctgc
     9721 gcccgcgcaa gggagcgcgg ctcaccgcca tcacctgcgg cggcgcgatc cctgacaacg
     9781 ccgactacca ggtgatcctc gagccggcgg gcatcttcgt cggtacgctc aacgaagatt
     9841 tcgccagcga gagcctcgcg ggcgacattt tccagctcgg caacacgtct tacaagattc
     9901 tgcgagtcga agccggccgc gtgcgtgtcg aagacgcgaa gggtcagcca cccagcat
//
LOCUS       DASQJS010000208         7084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1057595, whole genome shotgun sequence.
ACCESSION   DASQJS010000208 DASQJS010000000
VERSION     DASQJS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057595"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VEW08_08485"
     CDS             complement(<1..432)
                     /locus_tag="VEW08_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40812.1"
                     /translation="MQTDVLKAAREILPLITQLRSETESARRIAPPVVESLRKARLCR
                     LVIPRELHGLELPTPLALEVYELLAGAEASVGWIIWNNTLPCFWGRLLKPAARAEIFG
                     NPDWLYASSTRPSGKAVVEANGFRVSGRWSLVSGCELSEWLA"
     gene            621..917
                     /locus_tag="VEW08_08490"
     CDS             621..917
                     /locus_tag="VEW08_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40813.1"
                     /translation="MATATQSSWHVIARVCASIFGSYAFTWGFIALGASSLIIGGTAF
                     HEASSLTSMLGFLVYLWAFCWAFIAASLARVWVVLAGGGVVMTLAGWWLSRSIA"
     gene            952..2592
                     /locus_tag="VEW08_08495"
     CDS             952..2592
                     /locus_tag="VEW08_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HYJ40814.1"
                     /translation="MSFAWLHTWFGLVLGFVLMAAFFFGSLSVFDREIDRWALPETRI
                     EPQPMPSFDTILASRFAEIAAPEPEELAAASKRSTQPLPEKLKPALFGAYTTHRDPVL
                     DVFVGFEVPNSTLPNDLAYGSVVIDPRDGRKLPKDRLALGSAFFYPMHYSLHFTWYDI
                     GMWIVGLAAMAMLAAVVSGVVMHRKIFREFFTFRPGKKLLRGTLDLHNLTGVVALPFH
                     FLWALSGLIIFAGVYFPVFDKMLQPLVEQHEREEARHTGLDAEPSGVPGTLASVDAMM
                     IAAKDHWVSRGVPGEVGYLQVTHVGSSNAYVSIYRDSTDRVATAEGLHFQGSTGRVIF
                     EDPPTGAAETVNGFLTGMHLQQFRHWTLRWLIFVGGLLSCVCITTGFIFFVEKRKHVH
                     ARQGTSGSRWVDALAVTTVTGMMVATLATLVMNRALPEDLANRDLWEKSTFWIAWLTA
                     LTHALWRSAAVLQARMSKAWAEQSWAIAALGAVAVVLNAATTGDHLGRTLMAGYWPVA
                     GVDLMLIASACAAAIAARKLRARAAEVVPVNPEVPDYA"
     gene            2585..2905
                     /locus_tag="VEW08_08500"
     CDS             2585..2905
                     /locus_tag="VEW08_08500"
                     /inference="COORDINATES: protein motif:HMM:NF023232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3325 domain-containing protein"
                     /protein_id="HYJ40815.1"
                     /translation="MPDALLLAAALACTVIGMGWLSLAMDVHWIQVRATRRSPRMVII
                     LRVLGAASLAASLVLCLLADTATMAALVWMMLLAVAAVTIALVLSRKPALLAPLVAAF
                     PQSP"
     gene            2972..3490
                     /locus_tag="VEW08_08505"
     CDS             2972..3490
                     /locus_tag="VEW08_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40816.1"
                     /translation="MPRSFTLLLGASLLACMASLVTADEKPKRGCVAVEARQFDFWIG
                     RWAVTEKGKPAGTNHIERILDGCALLENWTGAQGGSGKSLNFFDRGDGKWHQTWVDGR
                     GGALFLAGTFENGAMRMEGERAAADKEPASRHRITWTALAGGTVRQLWESSPSDKEEW
                     TVQFDGLYTPIK"
     gene            complement(3483..4445)
                     /locus_tag="VEW08_08510"
     CDS             complement(3483..4445)
                     /locus_tag="VEW08_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYJ40817.1"
                     /translation="MILTEMPDLPPRPQTPRNAAFRREFYRHWGRENCIVSGVSRRVE
                     YRLFRQMLSIKCIARGDETYFVDRRRVTVSEDTYLVLNEGRTYGSVLETPAEAYSFSI
                     FFRPGFAQEVAGDLGRSLDQALDDGAEPAFGSIEFDESLHPHDRNISPVLRYIQRQVA
                     AGVRDEHWLEEQCQFLLGRLIAAHRRWRNSLPFELVNASPGKRTELARRLTLATDFMH
                     AHAADEITLKDIAAAAHLSRFHFLRLFRLVYGSTPVAALREMRTQRALALMESTTLTA
                     GEIAGRVGMSRIALWRSLRQKGSGVRQMRCAEGTARSAFLGRST"
     gene            complement(4550..6052)
                     /locus_tag="VEW08_08515"
     CDS             complement(4550..6052)
                     /locus_tag="VEW08_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYJ40818.1"
                     /translation="MKRRDFLNSSLLAAAITVPGIRAAYAAVGTAEVPDLAAITGDGR
                     EIMLRGKDIRDLAAKMRGQLLLAADEGYDKARLVLNPSFDKHPALIAQPSGVADVQAA
                     VAFARANNLLVAVKCGGHSASGQSSCDRGMQIDLSKFRGVRVDPRARRAWVEGGTLLG
                     EVDHECMAHGLVAPLGTVSHTGVGGLTTGGGFGRLVRRFGMSIDNLESVDLVTADGQL
                     RHASAKENPDLFWAVRGGGGNFGIVVNFEFRLHPMQREVVAGMVRFPIARAKDLLSMY
                     ADYVATAPDELYFDPVVVQPPGDAPGYTLLEVCYSGPQKNAETALAPIRKLGTPDRDT
                     IKAQDYVHVQRSNDSGDSRSRASYLKGGFISKVPDKLVSQLVDAFEGNPGRSTILYCQ
                     HCGGAAGRQPENATAFAQRDSIANMMTASSWQLAAGGPAEHIQATRKYWATLEPFTRG
                     FYVNDMAREASSSEINSNYRGNYKRLVAIKNQYDPTNLFRLNANIQPSKT"
     gene            6173..6631
                     /locus_tag="VEW08_08520"
     CDS             6173..6631
                     /locus_tag="VEW08_08520"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4437 domain-containing protein"
                     /protein_id="HYJ40819.1"
                     /translation="MRYSVVFFLCLLAAGSFAQMPDQEPAVVRTADDAALKWGPCPPF
                     LPEGCGIAVLHGDPAKDNVDVFLRLPARSQLPLHWHTSAERMVLVAGELHATYDGQEK
                     VVLKPGSYAFGPAKRSHSGVCASARPCILFIAFESPLDAVPIVGTEKPGS"
     gene            complement(6649..>7084)
                     /locus_tag="VEW08_08525"
     CDS             complement(6649..>7084)
                     /locus_tag="VEW08_08525"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HYJ40820.1"
                     /translation="YGFKIIDDLVDGLGNYLDTKGFASVGELVGKAVPSVTDWQYLNL
                     NHVDKAFIDQDLCIKCGRCHIVCEDTSHQAITATVNGARHFEVIDAECVGCNLCVSVC
                     PVEKCITMRPMTAGVDPRTGRAITNVKADWTTHPNNPGRKNA"
BASE COUNT         1233 a         2183 c         2287 g         1381 t
ORIGIN      
        1 ggccagccat tcggacaact cacaacccga gaccagcgac caacgtccgc tgacgcgaaa
       61 gccgttcgct tcaaccaccg ccttgccgct cggccgtgtg gagctcgcgt agagccagtc
      121 ggggttgccg aagatttccg cgcgcgcggc cggcttgagc aagcgtcccc agaaacacgg
      181 cagcgtgttg ttccagatga tccaacccac cgaggcttca gcacccgcaa gaagttcgta
      241 cacctcgagc gcgaggggcg tgggcagctc gagaccgtgc aactcgcgcg gaatgaccag
      301 gcggcacagc cgcgccttgc gcagggattc aaccaccggc ggcgcgatgc gccgcgcgga
      361 ttcggtttcg ctccgaagct gggtaatcag cggcaggatt tcgcgagccg ccttgagcac
      421 gtccgtttgc atgcccgcga tcgtacgacc cagcctgcgg gcggctcaaa tgcgaaaaac
      481 ttgtacatga gaaccgttct cattaaagtc ccgacacctt atctatagcg ccagccagcc
      541 agccggtccc gggttgcatg tcctcaacct gaccgcgccc gccagccagc tttcaacggt
      601 cctttcggac tggaacagcg atggcaacgg cgactcaatc ttcctggcac gtcattgcgc
      661 gagtttgcgc gagcattttc ggcagctacg cattcacctg gggtttcatc gcccttggag
      721 cgtcatcgct gatcatcggc ggaaccgcgt tccatgaagc ctcgagcctc acatccatgc
      781 tcggattcct ggtctacctg tgggcgttct gctgggcgtt catcgccgcc agtcttgcgc
      841 gtgtgtgggt ggtgctggcc ggtggcggcg tggtgatgac gctcgcgggt tggtggctct
      901 cgcgatccat cgcgtagccg cgggagactc aagttgttcg cgaactttcg attgtccttc
      961 gcttggctgc atacctggtt cggcctggtg ttgggattcg tgttgatggc cgcattcttc
     1021 ttcggctcgt tgtcggtgtt cgatcgcgag atcgatcgct gggcgcttcc cgaaacacgt
     1081 atcgaaccgc agccgatgcc gtcgttcgac acgattctgg cttcccgctt cgcggagatc
     1141 gctgcgcccg aaccggagga actcgctgcg gcgtcgaagc gctctactca accgctgcct
     1201 gaaaaactga agcccgcgct ctttggcgcc tacaccacgc accgtgatcc ggtactcgac
     1261 gtgttcgtgg gcttcgaggt tccaaattcc actttgccca acgaccttgc ctacggcagc
     1321 gttgtcatcg atccgcgcga cggtcgcaag ttgccgaaag accggctggc gctcggctcc
     1381 gcgttcttct acccgatgca ctacagcctg cacttcacgt ggtacgacat cggtatgtgg
     1441 atcgtggggc tggccgcgat ggcgatgctg gcggcggtgg tcagcggtgt cgtgatgcac
     1501 cggaagattt tccgcgagtt cttcacgttt cgtcctggca agaagctgct gcgcggcacg
     1561 ctggacctgc acaacctcac gggcgtggtc gcgcttccgt ttcatttcct gtgggcgctg
     1621 tcgggtctca tcattttcgc cggcgtctac tttccggtgt tcgacaagat gcttcagccg
     1681 ctggtcgagc agcatgaacg cgaagaagca aggcacaccg gcctcgacgc cgagccctct
     1741 ggcgtgccag gcacgctcgc gtcggtcgac gccatgatga ttgccgcgaa agaccattgg
     1801 gtgagccgcg gagtgcctgg cgaagtcggc tatctgcagg tgacgcacgt cggcagctcc
     1861 aacgcctacg tgagcatcta tcgcgacagt accgatcgcg tcgcgaccgc ggaggggttg
     1921 cactttcagg gctcgaccgg gcgcgtgatc ttcgaagacc caccgaccgg tgccgcagag
     1981 accgtcaatg gattcctgac cggcatgcac ttgcagcagt tcaggcactg gacgctgcgc
     2041 tggctgattt tcgtgggcgg ccttctgagc tgcgtgtgta tcaccacggg tttcatcttt
     2101 ttcgtcgaga agcgtaaaca cgtgcacgcc agacagggca ccagcggatc gcgctgggtg
     2161 gatgcgctgg ccgtcaccac cgtgacgggc atgatggtcg caaccctggc aactctcgtg
     2221 atgaaccgcg cgctgcccga agacctcgca aaccgcgacc tgtgggagaa gagcactttc
     2281 tggattgcct ggctgaccgc gctcacgcac gccttgtggc gttccgccgc ggtgctccag
     2341 gcgcgcatgt cgaaggcctg ggcagagcag agttgggcga ttgcggcgtt gggcgccgtg
     2401 gcggttgtat tgaacgcggc gacgaccggc gatcatctgg gccgcacgtt gatggcgggc
     2461 tactggcccg tggcaggagt cgacctgatg ctgattgcca gcgcatgcgc cgcggcgatt
     2521 gcggcgcgca agcttcgggc gcgagccgcc gaagtggtgc ccgtgaatcc ggaggtgcct
     2581 gactatgcct gacgcattgt tattggcggc ggcgctcgcc tgcacggtga tcggcatggg
     2641 ctggctatcg ctggcgatgg acgtgcattg gatacaggta cgcgcgaccc gtcgttcacc
     2701 gcgcatggtc atcattctcc gcgtacttgg agcggcgtcg ttggcagcct cgctcgttct
     2761 ttgcctgctg gccgacacgg ccaccatggc ggcgctcgtg tggatgatgt tgttagctgt
     2821 cgcggcggtg acgatagcgc tcgtgctgag ccggaagccg gcattgctgg caccgttggt
     2881 cgcggcattt ccgcaatcgc cgtaacaagg cgggcttgac gcattgaagc gagcagcgga
     2941 cgctgaagcg gaatgttttc aggagccagc catgccacgt tccttcacgt tactcttggg
     3001 cgcgagcctg ctggcttgca tggcgagcct ggtgacggct gacgaaaaac ccaagcgcgg
     3061 ctgcgttgcc gtcgaggccc gccagttcga cttctggata ggacgctggg cagtcacgga
     3121 aaaaggtaag ccggccggca cgaaccacat cgaacgcata ctcgacggct gtgcgctgct
     3181 ggaaaactgg actggcgcgc agggtggatc gggaaagagc ctcaatttct tcgatcgagg
     3241 cgatgggaag tggcaccaga cctgggtcga cggtcgcggt ggagcgctct tccttgcggg
     3301 cacgtttgaa aacggtgcga tgcgcatgga aggcgagaga gcggcggcgg acaaggagcc
     3361 cgcctcacgt catcggatca cgtggacagc gcttgcgggt ggcacagtgc ggcagctgtg
     3421 ggagtcgagc ccgagcgaca aggaagagtg gaccgttcag ttcgacgggt tgtacacacc
     3481 catcaagtag agcggcccag aaacgcagac cgcgcggtgc cttcggcgca gcgcatctgg
     3541 cgtacgccag agcctttctg acgcaggctg cgccacagcg caatacgcga catcccgacg
     3601 cggccggcga tttcacctgc cgtcagcgtg gtcgactcca tgagggccag cgcgcgctgc
     3661 gtgcgcattt cacgcaacgc ggcgaccggc gtgctgccgt agaccagccg aaacaggcgc
     3721 aggaaatgaa agcgcgacag gtgtgccgcc gcagcgatgt ccttgagagt gatttcatcc
     3781 gcggcgtgcg cgtgcatgaa atcggttgcc agcgtgagcc ggcgcgcgag ttcggtccgc
     3841 ttcccgggac tggcgttgac gagctcgaac ggcaggctgt ttcgccatct gcggtgggca
     3901 gcgatcaggc gccctaacaa gaactggcac tgctcctcga gccaatgctc gtcgcgtaca
     3961 cccgccgcca cctgccgctg gatgtagcgc aacaccggcg agatgttcct gtcatgcggg
     4021 tggagtgact catcgaactc gatgctcccg aacgcaggct ctgctccgtc gtcgagcgcc
     4081 tggtccagcg atcgcccgag gtcgccggcg acttcctgcg cgaacccggg acggaagaag
     4141 atggagaagc tgtaagcctc ggcgggagtt tcgagaacac ttccgtaggt gcggccttca
     4201 ttcagcacga gataagtgtc ttcgctcacg gtgacgcgcc gccggtctac gaaatacgtt
     4261 tcgtccccgc gcgcaatgca cttgatcgac agcatctggc ggaacagccg gtattcaacg
     4321 cggcgagaga cccccgacac gatgcagttt tcccgacccc agtgccggta gaactcacgg
     4381 cggaatgccg catttcgcgg cgtttgcggc cgcggcggca gatcgggcat ttcggtgagt
     4441 atcaccctgc catggttcga tgcgagaaag cctcgcgtca agccacattt gtttcaccca
     4501 ttgctgccgc cggtagccaa tgcgcaacag cggcttccga acgctggatt caggtcttgg
     4561 acggctggat gttcgcgttc agccggaaca ggttcgtcgg gtcgtactga ttcttgatcg
     4621 ccaccaggcg tttgtagtta ccacgatagt tggagttgat ctcgctcgag gacgcttcgc
     4681 gcgccatgtc gttgacgtag aacccgcgcg tgaacggctc gagcgtcgcc cagtacttgc
     4741 gcgtcgcctg gatgtgttcg gccggacccc cggcggccag ttgccaggag gaagcggtca
     4801 tcatgttggc gatggaatcc cgctgcgcaa aagccgtggc gttttccggc tggcgcccgg
     4861 cggcgccgcc gcagtgctgg cagtagagaa tcgtcgacct gcccggattg ccctcgaaag
     4921 catcgaccag ctgcgacacg agtttgtccg gaaccttgga aatgaagcca cccttgaggt
     4981 atgaggcgcg ggaacgcgaa tcccccgagt cattggagcg ctgtacgtgg acgtagtcct
     5041 gggccttgat ggtgtcgcga tccggcgtgc ctaacttccg aatgggggca agcgcggttt
     5101 ccgcattctt ttgcggaccg ctgtaacaga cttccagcaa tgtgtaaccg ggagcgtccc
     5161 cgggtggctg aaccacgacc ggatcgaagt agagttcgtc cggcgcggtc gcgacatagt
     5221 cggcatacat gctcaagagg tccttggctc tcgcgatcgg gaaacgcacc attcccgcga
     5281 ccacctcgcg ttgcatcggg tgcaggcgga attcgaagtt cacgacgatg ccgaagttgc
     5341 cgccgccacc gcgcacagcc cagaacagat ccggattctc cttcgcgctt gcgtgtcgaa
     5401 gttggccgtc ggccgtcacc aggtctacag attcgagatt gtcgatcgac atgccgaatc
     5461 ggcggacgag gcgaccgaaa ccaccgccgg tagtcaggcc gcccacgccg gtgtgcgata
     5521 ccgtgccgag cggcgccacg agcccgtgcg ccatgcactc gtgatccacc tcgccgagca
     5581 gcgtgccgcc ctcgacccag gcgcgccgcg cgcgaggatc cacgcgcact ccacggaatt
     5641 tcgacaggtc gatctgcatt ccgcgatcgc agctggactg gcccgacgcg ctgtggccgc
     5701 cgcacttcac cgcgaccagc agattgttcg cgcgcgcaaa ggcgactgct gcctggacgt
     5761 cggcaacgcc gctcggctgc gcgatgaggg cagggtgttt gtcgaacgag gggttcagaa
     5821 ccaggcgcgc cttgtcgtaa ccctcgtcag cggcgagcag gagctgcccg cgcatctttg
     5881 cggcgaggtc gcggatatcc tttccgcgca gcatgatttc gcgaccgtcg ccggtgatgg
     5941 cggccaggtc aggtacttcg gctgtgccga cagccgcata cgcggcccgg atgccgggta
     6001 cggtaatggc ggcggcgagg agagacgaat tcaggaaatc gcggcgtttc attgattgcc
     6061 tcactcttga tggtggctct ggcgctgtaa ctatctcatg gaattcccac gctggctccc
     6121 acgcccgggt cttaagctcg cgccgaacct gcaacctgga aaggagaggg caatgcgcta
     6181 ttcagtcgtt tttttcctgt gcctgcttgc cgcggggtcc ttcgcgcaga tgccggatca
     6241 ggaaccggcg gtcgttcgaa ccgccgatga tgccgcgctc aagtggggtc cctgtccgcc
     6301 gttcctcccc gaggggtgcg gcattgccgt tctgcatggc gacccggcaa aagacaacgt
     6361 cgacgtgttt ctccggctgc cggctcgatc gcagctccca ttgcattggc acacgtcggc
     6421 ggaacgcatg gtgctggtcg cgggggagtt gcacgccacc tacgatggcc aggaaaaggt
     6481 agtgctcaaa cccggcagtt acgcattcgg acccgcgaag cgttcccaca gcggcgtttg
     6541 tgcaagtgcc aggccttgca tcctcttcat cgcgttcgaa tctccgctgg atgcggtgcc
     6601 gatcgtcggc acggaaaaac ctggaagctg agctcagctt cggccgaatc aggcgttctt
     6661 gcgacccggg ttgttcgggt gcgtggtcca gtcggccttc acgttggtaa tggcgcggcc
     6721 ggtgcgcgga tcgacgcccg ccgtcatcgg acgcatcgtg atgcatttct ccaccgggca
     6781 gacgctgacg cagagattgc agcctacgca ctcggcgtcg atcacctcga agtgtcgcgc
     6841 gccgttgacg gttgccgtga tggcctggtg cgaggtgtct tcgcaaacga tgtggcaccg
     6901 gccgcacttg atgcacaggt cctggtcgat gaacgccttg tcgacgtgat tgagattgag
     6961 gtactgccag tcggtgacgc tgggcaccgc cttgccgacg agctcgccga ccgaagcgaa
     7021 gcccttggtg tcgagataat tgccgaggcc atcgacgagg tcgtcgatga tcttgaagcc
     7081 ataa
//
LOCUS       DASQJS010000209         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2629889, whole genome shotgun sequence.
ACCESSION   DASQJS010000209 DASQJS010000000
VERSION     DASQJS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2629889"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VEW08_08530"
     CDS             <1..543
                     /locus_tag="VEW08_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40821.1"
                     /translation="THQAGSFVESQLALAKKLGNRTEVTTPNLASLMFLVREGSQTLL
                     LTGDGHADDVLKGLAQRNLLDANGKLHVDVLKVPHHGAEFNMTTAFAQAVTADDYIFC
                     GNGFATNPELEVIDLLVKERKKAFPQKAFTLWFNSTSALTQPAYRKHMRAVEKNVADH
                     VNAGAGKVKARFISGSFMDV"
     gene            complement(566..1129)
                     /locus_tag="VEW08_08535"
     CDS             complement(566..1129)
                     /locus_tag="VEW08_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40822.1"
                     /translation="MNGRYTLIAVLTVTMLGIGAAVFAGGDGNRRDRDDEDARVRLGF
                     KLSPVPLKLRGRNLELVGLGSYIVNTGGCGECHTHPAFVAGGNPFLGQAEAINAEVFM
                     SGGRMFGPAIKAPNITPDEFGLPAGLDFAAFEATIRTGRKPDGSGRIMQVMPWPQMGK
                     RTTRELRAMYEYLRSIPSLPDSPNPGP"
     gene            1258..3141
                     /locus_tag="VEW08_08540"
     CDS             1258..3141
                     /locus_tag="VEW08_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HYJ40823.1"
                     /translation="MQPPEGRAIYDFGEFRLDAGQRLLTSRTDGRVLPLVSRAFDTLL
                     YLVERPGELVSKDALLKAVWPGTIVEENNLNQSIAAIRRAIGERAGEHKYIVTIPGRG
                     FKFVAPVSSATSLPGAAPAVLSTTVPIAAVRAPPGWKNYALAAIAAIAIFVAVFVVRH
                     PNDAPPAPATLAILPFKSISPTDEDASLRFGMTDTLISRLRELDGITVQPFSSVRRYG
                     NAEQDALEAARNLGVASVLDGTIQRSGDRLRVTTRLLNVADGRQLWSQQFDENFTDIF
                     TVQDTIAARVTDALAIRLTGQTDRRLTRRFTEDADAYQLYLSGWFQRSRAGEDGFRRS
                     IDFFEKAIARDPNYPLPYVGLADGYAMLGVFGALAPQEAFPRALVAANKALELDNELG
                     EAHTSLAHIKAVYERDLPGAEREYQLALSLAPDYAMAHMWYGLTLAWSGRYEDGLAQL
                     RQAQELEPLQLGASANIGMLMYFSRRYDESIQQLRKVLAVEPGLDHARSFLGRAYLRK
                     GDPAAAIAEFKLRKSLSVGSYADLGMALALAGRRDEAAAELDRLIALRQERYVSAYDI
                     AAVQASLGETDQAFEWLNRAFEERAQMRGLLGVDPSFDSLREDSRMKPLLERLRPPRA
                     EKT"
     gene            3141..3416
                     /locus_tag="VEW08_08545"
     CDS             3141..3416
                     /locus_tag="VEW08_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40824.1"
                     /translation="MRSSILMLTLLCGGAASCAAAPSPNAVTPMRASKIVLRPGRHDR
                     PAIAGMAQPKLSFDYTHLGRTGADYFSALVTVELALAVPDMRPLLIP"
BASE COUNT          630 a         1129 c         1130 g          659 t
ORIGIN      
        1 acgcatcagg cgggcagttt cgtcgagtcg cagctcgcgc tcgccaagaa gctcggtaac
       61 cgcacggaag tcaccacgcc gaatctcgcc tcgctcatgt tcctcgttcg cgaaggtagt
      121 cagacgctgc tgctcaccgg cgacggacac gccgacgacg tgctcaaggg cctggcacaa
      181 cgcaacctgc tcgatgccaa cggcaagctg cacgtcgatg tgctcaaggt tccgcaccac
      241 ggcgccgagt tcaacatgac caccgcgttt gcccaggcgg tcaccgccga cgattacatt
      301 ttttgcggca acggcttcgc cacgaatccg gagctcgaag tcatcgacct gctcgtcaag
      361 gagcgcaaga aagcatttcc gcagaaagcc ttcacgctgt ggttcaacag cacgtccgca
      421 ctaacgcagc ctgcttaccg caaacacatg cgggccgtcg aaaaaaacgt ggcagatcac
      481 gtgaacgccg gggcaggcaa ggtcaaggcc cgattcatct cgggcagctt catggacgtg
      541 taggcgcgcg gtggcttcac cgctttcaag gtcccggatt ggggctgtcg ggtaacgatg
      601 gaatcgagcg caggtactca tacattgcac gcaattcccg cgttgtcctc ttgcccatct
      661 gcggccacgg catgacctgc atgatgcggc ccgaaccatc gggtttgcgc cccgtgcgaa
      721 tggtcgcttc gaaggcggcg aagtcgaggc cagcagggag gccgaattcg tcgggcgtga
      781 tgttcggcgc cttgatggcg ggtccaaaca tgcgtccgcc ggacatgaat acctcagcgt
      841 tgattgcctc cgcttgaccc aggaacgggt tgccgcccgc cacaaatgca gggtgggtgt
      901 ggcattcgcc gcatccgccc gtgttgacga tgtagctgcc gaggcccacg agctcgagat
      961 tcctgccgcg cagcttcagc ggcaccgggg agagcttgaa ccccaggcgc acccgggcat
     1021 cctcatcgtc gcggtcgcgc cggtttccat caccgccggc gaatacggct gcaccgatgc
     1081 ccagcatcgt gacagttagg actgcaatca gggtgtatct gccattcacg agagtctcct
     1141 tttgttcggc cggccgtctt ggcggcgggc tatagttggg cgcaagagag gcgccccggg
     1201 ctttggccgg cgagtgaaat caactgaatc ttcgtgaaaa cctcaagcgg gccccacatg
     1261 cagcctcccg aagggcgcgc gatctacgac tttggtgaat ttcgcctcga tgccgggcag
     1321 cgcctgctga cgtccaggac cgacgggcgc gtgttgccgc tggtctcgcg cgcgttcgac
     1381 actcttctat atctggtcga acgccccggc gagctggtgt ccaaagacgc gctcctgaaa
     1441 gccgtgtggc ccggcacgat cgtcgaagaa aacaatctca atcagagcat tgcggccatt
     1501 cgcagggcga tcggcgagcg tgccggcgag cacaaataca tcgtcacgat ccccgggcgg
     1561 ggcttcaagt tcgtggcgcc ggtctcttcg gcgacttcgt tgccgggcgc cgcgccggcc
     1621 gtcttgtcga cgacagtgcc gattgcggct gtgcgcgccc cgccaggctg gaagaactac
     1681 gcgctggcgg cgatcgctgc gattgcgata tttgtcgccg tgttcgtggt gcgccacccg
     1741 aacgatgcgc cgcctgcgcc cgcgacgctc gcgattctac cgttcaagtc gatttcgccg
     1801 acggatgaag atgcgtcgct gcggttcggc atgaccgata cgctgatctc gcgactgcgc
     1861 gagctcgacg gcatcacggt gcagccgttc agttccgtgc ggcgttacgg aaatgccgag
     1921 caggatgcgc tcgaggccgc acgcaatctc ggtgtggcgt cggtgctgga cggaaccatt
     1981 cagcgaagcg gcgaccgcct tcgtgtcacg acgcgcttgc tcaacgtggc ggatggccgc
     2041 cagttgtggt cgcagcagtt cgacgagaac ttcaccgaca tcttcacggt gcaggacacg
     2101 attgcggcgc gcgtgaccga tgcactggcc atccgtctga ccggccagac ggatcgacgg
     2161 ctcactcgcc gtttcaccga agatgccgat gcctatcaac tctatctcag cggctggttt
     2221 cagcgcagtc gggcgggtga ggacgggttt cgacgctcca tcgacttctt cgagaaggcc
     2281 atcgcccgcg atccgaacta cccgctgccc tatgtcggac tggcggacgg ctacgcgatg
     2341 ctcggggtat tcggcgcgct ggccccgcag gaagcatttc cgcgggcgct cgtggccgcg
     2401 aacaaggcgc tcgagctcga taatgaactg ggtgaggcgc acacgtcgct cgcgcacatc
     2461 aaggccgtgt acgaacgtga tctgcccggc gccgaacggg agtatcagct cgcattgagt
     2521 ctcgcgccgg actacgccat ggcgcacatg tggtacggcc tgaccctcgc ctggtccggc
     2581 cgctacgagg acgggctcgc gcaacttcgc caggcgcagg agctcgaacc gttgcaactt
     2641 ggcgccagcg ccaacatcgg catgctgatg tatttctcgc gccgctacga tgaatcgatt
     2701 caacaactgc gcaaggtcct ggcggtcgag cccgggctgg atcacgcgcg cagcttcctg
     2761 ggccgtgcct atctgcgcaa gggcgatccg gcggcggcca tcgcggagtt caagctccgt
     2821 aaatcgttgt ccgtgggcag ctatgcggat ctcggcatgg cgctggcgct tgcgggacgc
     2881 cgggacgaag cggccgcgga actcgaccgg ctgattgcgc ttcggcaaga acgctatgtc
     2941 tcggcttacg atatcgcggc ggttcaggcg agcctgggcg aaaccgatca ggccttcgag
     3001 tggctgaatc gcgcattcga ggagcgtgca cagatgcgcg ggttgctggg cgtcgatcct
     3061 tctttcgaca gtttgcgtga agactcgcgc atgaagccat tgctggaacg gctgcgcccg
     3121 ccacgagcgg agaagaccta gtgcggtcat ccattctgat gctgacattg ttgtgtggtg
     3181 gcgctgcatc gtgcgcagcg gcgccctcgc ccaatgcggt gacgccgatg cgcgcctcga
     3241 agattgtgct gcgccctggg cggcacgatc ggcccgcgat tgccgggatg gcgcagccga
     3301 agttgtcgtt cgactacacg catctcggcc gaaccggcgc tgactacttt tcggcgctgg
     3361 ttaccgtgga gctggcactt gccgtaccgg acatgcggcc gctgctcatt ccctgatact
     3421 caatcgcgcc cgcgaccgcg gcccgtgcag aacggaacca gccccatcgc ggacttgatg
     3481 ccattcacga tcaactgctt gaactgcggc tgacccggga agccggaacc gtcggtgaac
     3541 gcggcgcc
//
LOCUS       DASQJS010000210         6286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1370887, whole genome shotgun sequence.
ACCESSION   DASQJS010000210 DASQJS010000000
VERSION     DASQJS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370887"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VEW08_08550"
     CDS             complement(<1..986)
                     /locus_tag="VEW08_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HYJ40825.1"
                     /translation="MSTSNYFRNASAVALTLTGACLLASCNGPPGPKPPPKIEGAEFV
                     KQLNHDLVGLSREANAAGWTQATDITVDTQYLNSQVTDRYLEYFSRKAGEAKAYDKDQ
                     LDASTARSLMLLKLGVSAPAPADATKRSELAALSTELDAMFGEGKYCPGPEKDGKKTE
                     CKNLDELGDTIATSRNYQELTEAWAGWHTISKPMRPKYQRFVELANEGARELGYDDLG
                     VLWRSRYDMPAKDFEKEASRLYGQVEPLYKSLHCYARKRLQQRYGKDKVPDGKPIPAH
                     LLGNMWAQQWNRIFDDLLKPYPGASIESADRLLQKQKWDAVKMTKSAESFYTS"
     gene            complement(983..2512)
                     /locus_tag="VEW08_08555"
     CDS             complement(983..2512)
                     /locus_tag="VEW08_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HYJ40826.1"
                     /translation="MAEPTTTTAVSPDYSRSFFGHPRGLATLFMTEFFERFTYYGLRA
                     MLVLFLAASATGANPGFGVDGATAGAVYALFTGAAYLCCIPGGWIADRLLGQRSAVFT
                     GGVFIAIGNFILAIPATPAVFYMGLAVVVLGIGLLKPNVSSVVGALYEGQAGARRDAG
                     FSIFYMGINLGAAAGPFIAGGLGEGWNWRLGFLTCGFAMLIGLAQFKMTEKYLGNAGY
                     APQVPAEQRKRSWTVVAAVAVAVLIGASLLFTRQNVPSETALAQGLFIAQVAIAFGFF
                     GYMLFFGGLTTIEKKRVGVIVLFFLSAALFWGGFEQQATTFNTFALDYTDRSWLGSMF
                     PDNAHPAAWYQSINPVCIIVFAPFFAWIWVALGARNLDPSAPLKMGMGLVLLGVGFLI
                     MMWAAQLVVSSGGKVGPTWLLLAYMIHTFGELCLSPVGLSNVTKLAPAKFVSSLMGTW
                     FLGAAIGNTVAGLVGGHSASLKADELPHQFLIMTLIGGGAGVFIMLLARPLRSWIGDR
                     K"
     gene            complement(2660..3445)
                     /gene="tatC"
                     /locus_tag="VEW08_08560"
     CDS             complement(2660..3445)
                     /gene="tatC"
                     /locus_tag="VEW08_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HYJ40827.1"
                     /translation="MSDEPKSEDLAEGTLMSHLLELRNRLMKAMLGILVLFVPCAIYA
                     NELFTLIAQPLIDKLPAGTSLIATNVVSPFMTPFKVAFYVSLFFAMPIVLYQIWAFVA
                     PGLYRREKRFAVPLLVSSVLLFYAGAVFAYFAVFPVMFSFLVGTAPSGVSYMPDMTSY
                     LDFVITLFFAFGVAFEMPVAVVLLVLTGLVNVETLGKNRGYVIIGIFVVAAFLTPPDA
                     ISQTIMAVPMYLLYEGGLAMARVMQKMRREAAEAAEKEEAKAS"
     gene            complement(3442..3900)
                     /gene="tatB"
                     /locus_tag="VEW08_08565"
     CDS             complement(3442..3900)
                     /gene="tatB"
                     /locus_tag="VEW08_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HYJ40828.1"
                     /translation="MFEVGFTELLLIFALALVVLGPERLPKLAQQVGRWVGRARAMAR
                     QFRDQLEEEAHNLETKVNIDPGIDTSLDPKPAPPPSAGQSSPTTGASNVPAPPLAEPE
                     PEEEFYPPDHHMHPSQRAADTPVETPVEAPAEQAEFDLTTSREDDSRRAP"
     gene            complement(3964..4197)
                     /gene="tatA"
                     /locus_tag="VEW08_08570"
     CDS             complement(3964..4197)
                     /gene="tatA"
                     /locus_tag="VEW08_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HYJ40829.1"
                     /translation="MGPIGFKELVVILVVVLLIFGAKKLKTIGSDLGSAVRGFKKSMN
                     EGDQEESVKQLKADAEFSDPEKARNAEKSETKA"
     gene            complement(4313..4789)
                     /gene="dtd"
                     /locus_tag="VEW08_08575"
     CDS             complement(4313..4789)
                     /gene="dtd"
                     /locus_tag="VEW08_08575"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HYJ40830.1"
                     /translation="MIALIQRVSEASVTVDERIVGKIGAGILALIGFERGDDMDGVSR
                     LLTRMLTYRIFADDGGRMNLSLTDTGGGLLLVPQFTLAADTHKGTRPGFSTAAAPELA
                     RSLFAQCLTLARARHAVVAAGEFGAHMRVALVNDGPVTFWLRMPPDDKELTVIPGP"
     gene            4880..5515
                     /locus_tag="VEW08_08580"
     CDS             4880..5515
                     /locus_tag="VEW08_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYJ40831.1"
                     /translation="MALRIPFIVLLALAPLAVQAQAPAVTGDVTRGKALAYTCNGCHA
                     IPNYKNVYPTYSVPKLHGQRPEYLLSALKAYKSGERSHGTMHSQASSMNEQDMADVAV
                     FLAGPEVLTASKNDVPADQRPKASEVCLACHGTNGVGITADYPTISGQHRDYLERALH
                     DYQKGGRKNAVMAGMAATLTAQDIEQLAAYYSSQKPSLSVVPKKTSFLSSN"
     gene            complement(5516..>6286)
                     /locus_tag="VEW08_08585"
     CDS             complement(5516..>6286)
                     /locus_tag="VEW08_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYJ40832.1"
                     /translation="DRERGKPRQIPAFIYRPASAAQPGARPVLIDIHGGPEGQAVPTF
                     NPFTQFLVREMGFVVITPNVRGSSGYGKSFLNLDNGEDREDSVKDIGALLVWIGAQRD
                     LDAKRVFVSGGSYGGYMSLASLVHFSDRLRGGIDVVGISNFVTFLESTSAYRRDLRRV
                     EYGDERLPRMRAYLQRISPLTNAARISKPLLVVHGLNDPRVPANESQQMVAKIRARGG
                     EVWYLAAKDEGHGFRKKANRDFYQRTIVTFLEKQLAAK"
BASE COUNT         1202 a         1979 c         1997 g         1108 t
ORIGIN      
        1 gaggtgtaga agctctcggc cgacttcgtc atcttcaccg catcccactt ctgcttctgc
       61 agcaggcggt cggcggattc gatgctggcg cccggatagg gcttcagcag gtcgtcgaaa
      121 atgcggttcc actgctgcgc ccacatgttg cctaacaagt gtgccgggat cggtttgcca
      181 tccggcacct tgtctttgcc atagcgctgc tgcaggcgct tgcgcgcgta gcagtgcagg
      241 ctcttgtaga gcggctcgac ctggccgtac aggcgcgagg cttctttctc gaaatccttc
      301 gcaggcatgt cgtaacgcga acgccacaac acgccgagat cgtcataccc gagctcgcgg
      361 gcgccttcgt tggccagttc cacgaaacgc tggtacttgg gccgcatcgg tttcgatatc
      421 gtgtgccagc cagcccaggc ttccgtgagc tcctggtagt ttcggctggt cgcgatggtg
      481 tcgccgagtt cgtcgagatt cttgcactcg gtcttcttgc catctttctc gggtccaggg
      541 cagtacttgc cttcgccgaa catggcgtcg agctcggtgg acaacgccgc gagctccgag
      601 cgtttggtcg cgtcggcggg cgcaggcgca ctcacgccga gcttcaggag catcagcgaa
      661 cgcgcggtcg atgcatcgag ctgatccttg tcgtaggcct tcgcttcacc cgccttgcgg
      721 ctgaagtact cgaggtatcg gtcggtgacc tgggagttga ggtattgcgt gtcgacggtg
      781 atgtccgtgg cctgcgtcca gcccgcggca ttcgcttcgc ggctgagccc taccaggtcg
      841 tggttgagct gcttgacgaa ttcggcgccc tcgattttcg gcggtggttt cggtcccggc
      901 gggccgttgc acgaggcgag caggcaggcg ccggtcagcg tgagagcgac ggccgacgca
      961 ttgcggaagt agttggacgt gctcattttc tgtctccgat ccagctgcgc agcgggcggg
     1021 cgagcaacat gatgaataca ccggcgccac cgccgatcaa cgtcatgatg aggaactggt
     1081 gaggaagctc gtcggccttg agcgaagcag agtgaccccc caccaggccg gcgaccgtgt
     1141 tgccgatagc cgcgccgaga aaccaggtgc ccatgaggct gctgacgaac ttcgcgggcg
     1201 cgagcttggt gacgttggag agcccgaccg gcgacagaca caactcgccg aaggtgtgaa
     1261 tcatgtaggc cagtagcagc caggtaggac ccaccttgcc accgctcgag accacgagtt
     1321 gcgcagccca catcatgatg aggaagccga cgcctaacaa caccaggccc atgcccatct
     1381 tgagcggtgc tgacgggtcg agattgcgtg cgcccagcgc cacccagatc caggcgaaga
     1441 acggcgcgaa gacgatgatg cagaccgggt tgatggactg gtaccacgcc gccggatgcg
     1501 cgttgtccgg gaacatgctg ccgagccagg agcgatcggt gtagtcgagc gcgaatgtgt
     1561 tgaacgtggt cgcctgctgc tcgaagcctc cccagaacag cgccgcgctc aggaagaaaa
     1621 ggacgattac gccaacgcgt ttcttctcga tggtagtgag gccgccgaaa aacagcatgt
     1681 agccgaagaa gccgaaggcg atggcgacct gcgcgatgaa caatccctgc gccagtgcgg
     1741 tttccgatgg cacgttctgg cgcgtgaaca gcaagctggc gccgatcagc acggcgactg
     1801 caacggcggc tacgactgtc cacgagcgct tgcgctgttc ggcaggcacc tgcggcgcgt
     1861 agcccgcgtt acccagatac ttctccgtca tcttgaactg ggcaagcccg atgagcatgg
     1921 cgaagccgca ggttagaaac ccgaggcgcc agttccagcc ttcaccgagg ccgcccgcga
     1981 tgaacggccc cgccgccgca cccaggttga tgcccatgta gaagatagag aacccggcgt
     2041 cacgccgtgc gcccgcctga ccttcgtaca acgcaccgac aacactcgag acgttcggct
     2101 tcagcaggcc gatgccgagc acgaccaccg caaggcccat gtagaaaacg gcgggcgtgg
     2161 cgggaatggc aagaatgaaa ttgccgatcg cgatgaacac gccaccggtg aagacggcgc
     2221 tgcgttgtcc cagcaggcga tcggcgatcc agcctcccgg tatgcagcac aggtaggccg
     2281 cgcccgtgaa cagcgcgtat accgcgccgg cggttgcgcc atcgactccg aagcccgggt
     2341 tcgcgcccgt cgcggaagcc gcgagaaaca gcacgagcat cgcgcgcagg ccgtagtagg
     2401 tgaacctctc gaagaactcg gtcatgaaca acgtggcgag tccacgcgga tgaccaaaaa
     2461 agctgcggct atagtcgggc gacacggctg tcgtcgtggt cggttcggcc attttttacc
     2521 cctggaaacc tgatgggcgc tgttgcgccg cgatggcgcc gagtctgact caagcgtacc
     2581 gacgccagca aggaggcctt gcgggcgaag ggcatgccaa caatggcttt gcttgctgct
     2641 ccggcggctt gacgcgccgc tagctcgcct tggcttcttc tttctcggcg gcttccgccg
     2701 cttcgcggcg cattttctgc atcacgcgcg ccatcgcgag gccaccctcg taaagcaggt
     2761 acatgggcac ggccatgatg gtctgggaaa tcgcgtccgg cggcgtgagg aacgcggcca
     2821 cgacaaagat gccgatgatc acgtagccgc gattcttgcc gagcgtttcc acgttcacga
     2881 ggccggtgag caccagcagt acgacggcca ccggcatttc gaacgccacg ccgaaagcga
     2941 agaacagcgt gatcacgaag tcgagataac tcgtcatgtc gggcatgtac gaaacgcccg
     3001 agggcgccgt gccgacgagg aacgaaaaca tcaccgggaa caccgcgaag taggcgaaga
     3061 ccgcgcccgc gtagaacaac agcacggaag aaacgagcag cggcacggcg aaccgttttt
     3121 cgcgccggta caggccgggc gcgacgaatg cccagatctg gtacagcacg atcggcatcg
     3181 cgaagaacaa cgatacgtag aacgccacct tgaagggggt catgaacggc gagaccacgt
     3241 tggtcgcgat aagcgaagtg cccgccggta gtttgtcgat cagcggttgc gcgatgagcg
     3301 tgaacagctc gttggcgtaa atcgcgcacg ggacgaagag caccaggatg ccgagcatcg
     3361 ccttcatgag acggttgcgc agctcgagca ggtgcgacat cagcgtacct tcggcaaggt
     3421 cttcgctctt cggctcgtcg ctcacggggc gcggcgcgaa tcgtcttcgc gcgaggtagt
     3481 tagatcgaat tcggcctgct cagccggtgc ttccaccggc gtctccaccg gcgtatccgc
     3541 cgcgcgctgc gacggatgca tgtggtgatc cggcgggtag aactcttcct cgggttcggg
     3601 ttcggcgagc gggggcgcgg gcacgttcga agcaccggtg gttggggaag actgcccggc
     3661 ggatggcggt ggcgccggct tgggatcgag cgaggtgtcg atgcccggat cgatgttcac
     3721 cttggtttcg aggttgtgcg cttcttcttc gagctggtcg cggaactgcc gtgccatggc
     3781 gcgtgcgcgc ccgacccacc ggcccacctg ctgggcgagt tttggcagcc gctccgggcc
     3841 cagcacgacg agtgcgagcg cgaagatcag cagcagttca gtgaatccaa cctcgaacat
     3901 ggagtgctcc gccgcgcggc ggtggtctgg tagtcgaaga ccggcgagga acgccgtggg
     3961 ctattaggcc ttggtctcgc tcttctcggc attgcgggct ttttcgggat cggaaaattc
     4021 ggcgtcggcc ttgagctgct tgaccgactc ctcctgatca ccctcattca tcgacttctt
     4081 gaaaccgcgc acggcggagc cgaggtcaga gccaatcgtc ttgagcttct tcgccccgaa
     4141 aatcagcaaa acgacgacca ggatgactac aagttcttta aaaccaattg ggcccatgag
     4201 gcgctcctaa aggacgtcgt gacgccccag ataaatgcga tagataagtg ccggaatgat
     4261 aagtcaacag aggccccaaa cctaagaaag ttcggccggg gcgagactcc catcacggcc
     4321 cggggataac cgttaattcc ttgtcatccg ggggcattct gagccagaag gtcaccggcc
     4381 cgtcgttcac cagcgcaacc cgcatgtggg cgccgaattc gccagccgcg accactgcat
     4441 ggcgcgcgcg cgccagtgta agacactgcg cgaacaggga ccgggcgagc tcgggcgcag
     4501 ccgcggtgct gaacccgggc cgcgtgccct tgtgggtatc cgcggccagc gtgaactgcg
     4561 gcaccagcag cagcccgccg ccggtatccg tcagcgagag attcatgcgg ccaccatcgt
     4621 cggcaaagat tcgataggtg agcatgcgcg tgagcagccg ggagacgccg tccatgtcat
     4681 cgccgcgctc gaaaccgata agggcgagga tgccagcacc gatcttgccc acgatcctct
     4741 catcgaccgt gaccgacgct tcgctgaccc tctggatgag tgctatcaag atgttccgat
     4801 tgtgcccggt ggcaattgcg cgcccaagtc cttaatatcg cggcctgttt ttagggagcc
     4861 acggggcaac acttcagtca tggcattgcg tattccgttc atcgtccttc tggccctggc
     4921 accactcgcg gttcaggccc aggctcccgc cgtcaccggc gacgtcacgc gcggcaaggc
     4981 gctggcctac acctgcaacg gctgccacgc catccctaac tacaagaacg tgtatcccac
     5041 gtattcggta cccaagctgc acggccagcg cccggaatac ctgctctccg cgctgaaggc
     5101 gtacaagagc ggcgagcggt cgcacggcac catgcattcc caggcgtctt cgatgaacga
     5161 gcaggacatg gcggatgtcg ccgtcttcct ggccggcccc gaagtgctca ccgcttcgaa
     5221 aaacgacgtg ccggccgacc agcgcccgaa ggcaagcgaa gtgtgccttg cctgtcacgg
     5281 caccaacggc gtcggcatca ccgctgacta cccgacgatc tccggccagc accgcgacta
     5341 tctcgaacgc gcgcttcacg actaccagaa gggtggccgc aagaatgccg tcatggcggg
     5401 catggccgcc acgctcacgg cgcaggacat cgaacagctc gcggcttact actcgagcca
     5461 gaagccatcg ctgtcggtcg ttcccaagaa aacctcgttc ctctccagca actgactatt
     5521 tcgcggcgag ctgcttttcc aggaacgtga cgatggtccg ctggtagaag tcgcggttcg
     5581 ccttcttgcg gaatccgtgg ccctcgtcct tcgcggcgag gtaccacacc tcaccaccac
     5641 gcgcgcggat cttcgccacc atctgctgcg attcgttcgc gggtacgcgc ggatcgttga
     5701 ggccatgcac caccagcagc ggcttggaga tgcgcgcggc gttggtcagc ggtgaaatcc
     5761 tctgtagata ggcacgcatg cgcggcagcc gctcgtcacc gtactccacg cggcgcaggt
     5821 cgcgccggta ggccgaggtg ctctcgagga aggtcacgaa gttcgagatg cccaccacgt
     5881 cgatgccgcc gcgcagccgg tcgctgaagt gaaccagtga agccagcgac atgtagccgc
     5941 cgtacgagcc gcccgagacg aacacgcgtt tcgcgtccag gtcgcgctgg gctcctatcc
     6001 acaccagcag cgcaccgatg tccttgaccg agtcctcgcg gtcctcgccg ttgtcgagat
     6061 tgaggaagct cttgccgtag ccggaagaac cgcgcacgtt gggcgtgatc accacgaagc
     6121 ccatttcgcg caccaggaac tgcgtgaacg ggttgaaggt gggcacggcc tgtccctcgg
     6181 gcccgccgtg gatgtcgatc agcacgggac gcgcgccggg ctgggcggca cttgcgggac
     6241 gatagatgaa ggcggggatc tgccgcggct tgccgcgctc gcggtc
//
LOCUS       DASQJS010000211         8316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3889909, whole genome shotgun sequence.
ACCESSION   DASQJS010000211 DASQJS010000000
VERSION     DASQJS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3889909"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VEW08_08590"
     CDS             <1..258
                     /locus_tag="VEW08_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40833.1"
                     /translation="SFLSHHGIPASKYHATLTRAWILAVHHFMHRSPEASSADDFIAR
                     NPALLDSKIMLTHYSADLLFSDEARAGFVEPNLDPIPRHAA"
     gene            383..919
                     /locus_tag="VEW08_08595"
     CDS             383..919
                     /locus_tag="VEW08_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HYJ40834.1"
                     /translation="MHTRKFILTFAAALLVSASAHADDAGFYLGAGIGEARQHNDVFD
                     GSDTSFRWLAGYSFSRYFAAEAGFIDAGTQEDTIGAFDVKASSDGVFAAVLAKLPLGK
                     VVAPYAKLGYVFYDGAETVSTGGAISRESTSGEELLFGGGLEFRIGGHVRLRAEYEKV
                     DVPYADFEIYSAAFTFQF"
     gene            1002..2219
                     /locus_tag="VEW08_08600"
     CDS             1002..2219
                     /locus_tag="VEW08_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40835.1"
                     /translation="MTTRIIKLHHAVIATLLPWLAPLATAQDVQAPAEEPLVVQEITC
                     AGNEQTSCDFIRDHLYLNAGDALDEEQIRNAELRMSALRNFESVKFRLEKGARRGAVI
                     VVIEVEEASPIVREWLLGGSSRTDSERGVFAGRLAHQNLFGKGKIVDFTGVAVVPIGG
                     DAVNEAYDVSLRYVDPQLFDSSRYFAVAGARWRKRRYEDIYGNFGSMDAGQLEFTLGR
                     RIADFSYLSLGITFRPSNDWVSGRWDSDGSFVISSPESYGNPGVTIAYGWSTEDDIHF
                     PTQGTTFQIDAGGDYEPSEPEGRSHVQFRKTWARAGAYWTLKLGGDPSPEYRTSLGES
                     QLFALSYARPVTPGDIVQRGRWYIEPGFAIKGYTDSGTVFYEYGLKAGFRADTRIFGY
                     IDLYLLATKDGSR"
     gene            2216..2839
                     /locus_tag="VEW08_08605"
     CDS             2216..2839
                     /locus_tag="VEW08_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40836.1"
                     /translation="MRHAIALLVLLANVDVDAAEVTATFTDGKLGARVDALPFPAGLP
                     KELTSGLTNRLYARVSVADARATLGQKVVEIAVRYDLWDQTFSVVSTLDGNIADSRTL
                     ASLPEVNALLSALPLPRLFEVATLPPTDLTLRVEVLLNPIDREKMQMIRKWVAQNSMP
                     EVAGRQGITVSNAIFNRIFEQYADGSNVAAAWRAAVTSQPFRIAPEK"
     gene            2836..3945
                     /locus_tag="VEW08_08610"
     CDS             2836..3945
                     /locus_tag="VEW08_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYJ40837.1"
                     /translation="MKDARVGLLLTVSIFVMAAMPLAAAFYLLQDALRISLNLGFNPQ
                     VVRVLEDSSDNLRTLGRLDEANRAQYRSQFDEVEELKQIYSEPELVKQRILDSLQLYF
                     GLGLGAAVLLSVGVASLLSRRIARSHALNIEELSRERDKVRYLQEMSSWQELAKMLAH
                     EIKNPLTPIEVLVTSLSKAYLSKGQPEFLAHLTETQTMISEELQHLKSTVNKFSEFAR
                     MPQVQLADVDLVEELDRQIKVLSNAAGSADFEMHKGATQAVRVRLDVTLFRQVLANIV
                     RNGVEANPGRRVRFTFTIAAGSRASIRIANDGIPVQPEIVDRMFDPYISTKTGKDNMG
                     LGLAIVKKIVLEHGGDIRYEESHGHPAFVVELPCP"
     gene            3936..5285
                     /locus_tag="VEW08_08615"
     CDS             3936..5285
                     /locus_tag="VEW08_08615"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYJ40838.1"
                     /translation="MPVVLVLDDEKNIRNAIEIALSQEGIHVIGAHDVAAALRALHER
                     IIDAMIVDIRLGEVSGLEFFRRAEADGIAPPTIFISGHASLTEAAQTVKIGAFDFLEK
                     PFTAEKIAVTVKRCLEMSSLRERLRLAQSQRGGGEIVGDSAAIRRVVAAAMKVAETNA
                     TVLITGESGSGKELVANCIHANSERAAEPFVKVNCSAISESLVESELFGHERGAFTGA
                     VASRKGLFEIAHGGVIFLDEIADLAASAQAKILRVLQNGEIQRVGSERVTTVDVRILS
                     GTHKDLKVAVAEGRFREDLFYRLNVVPIRVPALRERKEDIPLLVQFLMRSLSQRNNMR
                     EKPIDDEVIEELKRHDWPGNVRELQNLLERLMIMSGERITTFDLPEEFLALPERGGAA
                     GSSLKEYRDHAEREYIVATLRRHNGNVSQAAIELGVGRTYLHRRLSVLQITRKDFLA"
     gene            complement(5282..6967)
                     /gene="leuA"
                     /locus_tag="VEW08_08620"
     CDS             complement(5282..6967)
                     /gene="leuA"
                     /locus_tag="VEW08_08620"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HYJ40839.1"
                     /translation="MLKDPSNKYRQFPPIDLPNRQWPTRQLTTLPVWCSTDLRDGNQA
                     LFEPMSRETKKRLFKTLVGVGLKEIEVGFPSASDTDFQTVRDLIEQKLIPDDVTIMVI
                     TQAREELIRRTIESLRGARRAIVHVYNATAPVWRRVVFGMSVPEVMQLITKQVGLIKT
                     LTDAQPETEWLLEYSPETFSMTELPVALEACNAATRAWDAGPGRPIIVNLPTTVENAT
                     PNIFADQIEWMSTRLERREHVVLSVHTHNDRGTGVAAAELGMLAGAQRVEGCLFGNGE
                     RSGNVDLVTLALNFYSQGIHPGLDFSDINSVARAVEEATRLPIHPRHPYVGDLVFTAF
                     SGSHQDAIKKGMAAQKPDALWEVPYIPVDPADLGRTYDSVIRVNSQSGKGGIAYLLER
                     DYGVVMPRRMQVEFSALVQREMDSSETEMTSQQLWALFEATYLATDLEVTYHAHHLFE
                     EGDKQGIELEVTVAGSRRKLRGVGSGPIAATVSALALPIRIDNYEERALRAGADASAL
                     AIVEAAREGVAGIRYGAGRHANIATASVLAVLSAARRLGVSASDLRMRGGDAG"
     gene            complement(7242..>8316)
                     /locus_tag="VEW08_08625"
     CDS             complement(7242..>8316)
                     /locus_tag="VEW08_08625"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ40840.1"
                     /translation="SYIAHMRSEGERLLEAVDETIDIARRAQIPVEIYHLKAAGKSNW
                     DKMDQVIARIDKARAEGLHVTTDMYTYTAGATGLDAAMPTWVQDGGLEAWVKRLKDPK
                     IRARVLAEMRAPGKDWENLFLAAGSADNILFIGFKTEKLKPLTGKRLSEVAKMRGKSP
                     EETAIDLVIEDGTRVDTVYFLMSEDNVRKQIKLPYMSFGSDAESSTPEGVFLQASTHP
                     RAYGNFARLLGRYVRDEKLISLPEAVRRLTSLPAGNLALKDRGLLKAGYFADIVVFDP
                     ATIQDHATFEKPQQFSTGVSEVVINGGVALSNGEPTGKATGRVVRGQGWKGWPDGGCR
                     ASAKDWPTREKGDRSIFRSEPGM"
BASE COUNT         1533 a         2596 c         2607 g         1580 t
ORIGIN      
        1 agctttctct cgcaccacgg catacccgcg tcgaagtatc acgccacgtt gacacgcgcg
       61 tggatcctgg ctgtccatca cttcatgcat cgctcgcccg aggcttcatc ggccgacgac
      121 ttcatcgctc gcaatccggc gcttctcgac agcaagatca tgctcacgca ttactctgct
      181 gatctcctgt tttcggatga agcgcgcgcg ggtttcgtgg aaccgaacct ggatccgatt
      241 ccgcgccacg cggcgtagcc tgctgccgca ggcacacctg tccacttagt tagacactct
      301 cgcgcacaaa atcgcgcgta caggcgcccg ccttgctggc atgcgtattg caattctccc
      361 tgcagctaaa cagggagatc gcatgcatac caggaaattc attctcacct ttgccgccgc
      421 gctgctcgtg tcagctagtg cacatgccga cgacgccggc ttctacctcg gcgcgggcat
      481 aggtgaagcc aggcagcaca atgacgtgtt cgacggcagc gatacttcgt tcagatggct
      541 ggccggctat tccttcagcc ggtacttcgc cgccgaagcg ggcttcatcg acgccggcac
      601 gcaggaagac accatcggcg cgttcgacgt aaaggcgagc agcgacggcg tattcgcggc
      661 cgtactcgcc aaactgccac tgggaaaggt cgtcgcgcct tacgccaagc tcggctatgt
      721 cttctacgac ggcgcggaaa ccgtttcgac gggcggcgcg atctcgcgcg agtccacgag
      781 tggagaagaa ctgttgttcg gtgggggtct cgaattccgg atcggcgggc atgtaaggct
      841 tcgggccgaa tacgagaagg tcgatgtgcc ctacgccgac ttcgaaatct actcggccgc
      901 gttcactttc cagttctgac atatcaccgc gcgatgggtg tccactttcg tggacatccc
      961 gccagcgcgc ggccagctgt tacatttcag gacggcctgc catgacgacc agaatcatca
     1021 aactacatca cgcggtcatc gccacgctgc tgccctggct cgcgccgctg gcgactgccc
     1081 aggacgtcca ggcacccgcc gaagagccgc tggtcgttca ggagatcacc tgcgcgggca
     1141 atgagcaaac gtcgtgtgat ttcatccgcg atcaccttta tctgaacgcg ggcgacgcgc
     1201 tcgatgaaga gcagatccgc aacgccgagc tgcgcatgtc ggcgctgcga aatttcgagt
     1261 cggtgaaatt ccggctcgag aaaggcgcca ggcgcggcgc tgtcatcgtg gtgatagaag
     1321 tcgaagaggc gagtcccatc gtcagggagt ggctgctcgg cggatcctcg cgtacggact
     1381 cggagcgcgg cgtattcgca ggacgcctcg cgcatcagaa cctgttcggc aagggaaaga
     1441 tcgtcgactt cacgggcgtg gccgtcgtcc cgatcggcgg tgacgccgtc aacgaggcct
     1501 acgacgtctc gctgcgatac gtggatccgc agctgttcga ttcgagccgc tatttcgcgg
     1561 tcgcgggcgc gaggtggcgc aaacggcgat acgaagatat ctacggcaat ttcggaagca
     1621 tggacgcagg acagctcgag ttcacgctgg ggcggcgcat cgccgacttt tcctacctgt
     1681 cgttgggcat cacgttccgg cccagcaacg actgggtttc cggccggtgg gatagcgatg
     1741 gctcgttcgt gatctcctcg cccgagtcgt acggcaatcc tggagtgacc atcgcctacg
     1801 gatggagcac cgaagacgat attcactttc cgacgcaggg tacgaccttt cagatcgacg
     1861 ccggcggtga ctacgaaccg agcgagcccg aaggccgctc gcatgtccag ttcagaaaga
     1921 cctgggcgag agcaggcgcg tactggaccc tcaagctggg cggcgatccg agcccggaat
     1981 accgaacctc gttaggtgaa agccaactgt ttgcgttgag ttacgcgcgg ccggtgactc
     2041 ctggcgacat cgtccagcgc ggtcgctggt acatcgagcc tgggttcgcc atcaagggat
     2101 acacggactc cgggacggtc ttctacgaat acggcctcaa agccggattt cgcgccgaca
     2161 cccggatatt cgggtacatc gacctgtacc tgctggccac gaaggatggc agccggtgag
     2221 gcacgccatc gcactgctcg tgctgctggc gaacgtggat gtcgacgccg cggaagtgac
     2281 ggcgacattc accgacggaa agctcggagc ccgcgtcgac gcactccctt tcccggccgg
     2341 gctgccgaaa gagctgacca gcggcctcac aaaccgcctc tatgcgcggg tgtcggtggc
     2401 cgatgcgcgg gccacgcttg gccagaaggt ggtcgaaatc gccgttcgct acgatctctg
     2461 ggaccagact ttttccgtgg tcagcacgct ggacggcaac atcgccgact cccgcacgct
     2521 cgcaagcctt ccggaagtca atgcgctgct ctcggcactg ccgttaccgc gcctgttcga
     2581 agtggccacg ttgcctccca ccgacctcac actgcgcgtc gaagtgctgc tgaatcccat
     2641 cgatcgcgag aagatgcaga tgatccgcaa atgggtggct cagaacagca tgccggaagt
     2701 ggccggccgg cagggaatca cggtgtcgaa tgccattttc aaccgaatct tcgaacagta
     2761 cgccgacggc tcgaatgtcg cggcggcctg gcgcgccgcc gtgacgagcc agccgttccg
     2821 gatcgcgcca gaaaaatgaa ggacgccaga gtcgggctgc tgctgacggt ttccatcttc
     2881 gtgatggccg cgatgccgct cgccgccgct ttctatctac tgcaggacgc gctgcgcatc
     2941 agcctcaatc tcggcttcaa cccgcaggtc gtgcgcgtgc tcgaagactc gtcggacaac
     3001 ctgcgtacgc tcggtcgtct cgatgaggcg aatcgcgcgc agtatcgctc gcagttcgac
     3061 gaagtcgaag agctcaagca gatctactcc gaaccggaac tggtcaagca gcgcattctc
     3121 gattcgctgc agttgtattt cggcctgggg ctcggcgccg cggtgctgct gtcggtcggt
     3181 gtcgcctcgt tgttgagccg caggattgcg cgcagtcatg cgctcaatat cgaagagctg
     3241 tcacgcgaac gtgacaaggt gcgttacctg caggaaatgt cgtcatggca ggagctcgcg
     3301 aagatgctcg cgcatgaaat caagaatccg ctgacgccca tcgaggtcct ggtcacttcg
     3361 ctgtccaagg cgtacctgtc gaaaggtcaa ccggagttcc tcgcgcacct gaccgaaacg
     3421 cagacgatga tcagcgagga attgcagcat ctgaaatcga cggtcaacaa gttcagcgaa
     3481 ttcgcgcgca tgccgcaggt gcagctggcg gacgtcgatc tcgtcgagga gctcgaccgg
     3541 cagatcaaag tgttgtcgaa cgccgccggg agcgctgact tcgagatgca caagggcgcg
     3601 acccaggcgg ttcgcgtgcg gctcgacgta acactgttcc gccaggtgct cgccaacatt
     3661 gttcgcaacg gcgtcgaggc aaacccgggc cggcgcgtgc gtttcacatt cacgatcgcg
     3721 gctggttccc gcgcaagcat ccgcatcgcc aacgacggca ttccggtgca acccgagatc
     3781 gtggatcgca tgttcgatcc ctacatctcg acgaagaccg gcaaagacaa catgggtctc
     3841 gggctcgcca tcgtgaagaa gatcgtgctc gagcacggtg gcgatattcg ctacgaggaa
     3901 agccacggcc atccggcgtt tgtcgtggag ctgccatgcc cgtagtgctg gtgctcgacg
     3961 atgagaaaaa catccgcaat gcgatcgaga tcgcgttgag ccaggagggc attcacgtga
     4021 ttggcgcaca cgacgtcgcc gccgcgttgc gcgcactgca cgagcggatc atcgacgcga
     4081 tgatcgtcga catacgcctg ggtgaagtca gcgggctcga attcttccgt cgcgctgaag
     4141 cagacggcat cgccccgccg accattttca tttccggtca cgccagcctg accgaagcgg
     4201 cgcagacggt gaagatcggc gcgttcgatt ttctcgagaa gccctttacc gccgagaaga
     4261 tcgccgtcac ggtgaagcgc tgcctcgaaa tgtcgagtct ccgggagcgc ctgcgcctcg
     4321 cgcagtcgca gcgtggcggc ggcgaaatcg tcggtgattc ggctgccatt cgcagagtgg
     4381 ttgcggcggc aatgaaggtc gcggagacca atgctaccgt gctgatcacc ggcgaaagcg
     4441 gctctggcaa agagctggtt gccaactgca tccacgcaaa cagcgagcgc gccgccgaac
     4501 ctttcgtcaa ggtgaactgc tcggcgattt ccgagagcct cgtggaaagc gagctgttcg
     4561 gccacgagcg cggcgcgttc accggcgcgg tggcctcgag aaaagggttg ttcgagatcg
     4621 cgcacggtgg cgtcatcttt ctcgacgaga tcgcggatct cgcggctagc gcgcaggcga
     4681 aaatcctgcg cgtcctgcag aacggtgaaa ttcagcgggt cggctcggaa cgggtgacca
     4741 cggtggacgt gcgcatcctc tccggaacgc acaaggatct gaaggtggcc gtcgccgagg
     4801 ggcggtttcg cgaagatctt ttctatcggc tgaatgtagt gccgatccgt gtgcccgcac
     4861 ttcgggaacg gaaggaagac atcccactgt tggtccagtt cctgatgcgg tccctgagcc
     4921 agcgcaacaa catgcgtgag aagcccattg acgacgaggt gatcgaggaa ctcaaacgcc
     4981 acgattggcc gggaaacgtg cgcgaactgc agaacctgct cgaacggctc atgatcatga
     5041 gcggcgagcg catcaccacg ttcgatctcc cggaagaatt ccttgcgctg ccggagcgtg
     5101 gcggcgctgc cggctcctcc ctgaaggaat accgcgatca cgccgaacgc gaatacatcg
     5161 tggcaacgct gcgccggcac aacggcaacg tgagccaggc cgccatcgaa ctgggtgtgg
     5221 gccgcaccta tctgcaccgg cgtttgtccg tgctgcagat caccaggaag gacttcctgg
     5281 cttagccggc gtcgccgccg cgcatccgaa gatcgctggc gctgacgccc agcctgcgtg
     5341 ccgccgacaa taccgcgagc accgaagccg tcgcgatgtt cgcatggcgg ccggcgccat
     5401 aacggatgcc ggcgacacct tcgcgcgccg cttcgacgat ggcgagcgcc gacgcgtccg
     5461 cacccgcgcg cagtgcgcgc tcttcgtagt tatcgatgcg gatgggcagc gcgagcgcgc
     5521 tcacggtcgc cgcgatggga cccgagccga cgccgcgcaa tttgcggcga ctgcccgcca
     5581 cggtgacctc gagctcgatg ccctgcttgt cgccttcctc gaacaggtga tgcgcgtgat
     5641 aggtcacttc gaggtcggtc gcgaggtagg tggcttcgaa cagcgcccac agctgctgtg
     5701 aggtcatctc ggtttcggac gaatccatct cacgttgcac cagcgcgctg aactccacct
     5761 gcatgcgcct cggcatcacg actccgtagt cgcgttcgag aagataggcg atgccgccct
     5821 tgccggactg gctgttcacg cggatcacgc tgtcgtaggt gcggcccaga tccgccggat
     5881 cgaccggaat gtagggcact tcccagaggg catcgggttt ctgcgccgcc atgcctttct
     5941 tgatggcgtc ctggtgagag ccggagaagg ccgtgaagac cagatcgccg acgtaggggt
     6001 gccgcggatg aatgggcagt cgcgtggcct cttcgactgc gcgcgccacg gaattgatgt
     6061 cggagaaatc caggcccggg tgaatgccct gggaataaaa attgagcgcc agcgtcacca
     6121 ggtccacgtt gccggagcgt tcgccattgc cgaacaggca gccttcgacg cgctgcgcgc
     6181 cggcgagcat gccgagctcc gccgcagcga cgcccgtgcc gcgatcgtta tgggtatgca
     6241 ccgagagcac cacgtgctcg cggcgttcca ggcgcgtgga catccattcg atctggtcgg
     6301 caaagatatt cggcgtggcg ttttccacgg tggtcggcag attgacgatg atcgggcgac
     6361 ctggacccgc gtcccaggcg cgcgtcgccg cgttgcaggc ctcgagcgcc accggcagct
     6421 cggtcatgct gaaagtctcg ggtgaatact cgagcaacca ttcggtctcg ggctgcgcat
     6481 cggtgagagt cttgatcaag ccaacctgct tggtgatgag ctgcatgact tcgggcacgc
     6541 tcatgccgaa cacgacgcgt cgccacactg gcgccgtcgc gttgtagacg tgcacgatgg
     6601 cgcggcgtgc gccgcgcaga ctttcgatgg tgcggcggat cagttcctcg cgcgcctgcg
     6661 tgatgaccat gatcgtcacg tcatcgggaa tcagtttctg ctcaatgagg tcgcgcacgg
     6721 tctggaaatc cgtgtcggac gcggacggaa atcccacctc gatttccttg agccctacac
     6781 cgaccagcgt cttgaacagc cgcttcttgg tttcgcggct catgggctcg aacagcgcct
     6841 ggttgccgtc gcgcaggtcg gtgctgcacc acaccggaag cgtagttagc tggcgcgtgg
     6901 gccactgccg gttgggcaga tcgatggggg gaaactggcg gtatttgttc gatggatctt
     6961 tgagcatggg cgtattactc gaaacgaaac ggctgggaac tacaggcgcg acttctccgc
     7021 atttgcggag tcttcggggt tccagtcttg tttgcgccga ctggtagcgc gtgtgtatgt
     7081 gcgcctaggg cgcaagaagg aggagcgaac ggagcagcgc gggtgccgcg gcgcggccct
     7141 gcgtggggtg acccatctgc tcgttgcgat tcatgcgtgg caacctacca cggatgcggg
     7201 cgcgcgaaaa aggggacaga tctatttttc gtccccttca gtcacatccc gggttcagat
     7261 cgaaaaatag atctgtcccc tttttcgcgc gtgggccagt ctttcgcgga ggcgcggcag
     7321 ccaccgtccg gccatccctt ccagccctgc ccacgcacga cgcgacccgt cgcctttccg
     7381 gtcggctcgc cattcgacag cgcgacgccg ccgttgatca cgacttccga cacgcccgtc
     7441 gagaactgct ggggtttctc gaacgtcgcg tgatcctgga tcgtcgcggg atcgaacacg
     7501 acgatgtccg cgaaatatcc agccttcagc aggccgcgat ccttgagcgc gagattgccc
     7561 gcgggcaacg acgtgagacg gcgcaccgcc tccggcagcg agatgagttt ttcatcgcgc
     7621 acataacggc cgagcaggcg cgcaaagttg ccgtacgcgc gcgggtgcgt gctcgcttgc
     7681 aggaacacac cttccggtgt cgacgattcg gcatccgatc cgaaactcat gtacggcagc
     7741 ttgatctgct tgcgcacgtt gtcttcggac atcaggaaat acacggtatc aacgcgcgtg
     7801 ccgtcttcga tcacgaggtc gatcgccgtt tcctcgggac tcttgccgcg catcttcgcc
     7861 acttcggaca gccgcttgcc ggtcagcggc ttgagcttct cggtcttgaa cccgatgaac
     7921 aggatgttgt cggcgctgcc cgcggccagg aacaggtttt cccaatcctt gccgggcgct
     7981 cgcatctccg cgagcacacg cgcgcggatc ttcggatcct tcagccgttt cacccaggct
     8041 tcgagtccgc cgtcctggac ccaggtcggc atcgcggcgt cgaggccggt cgcgcccgcg
     8101 gtgtacgtgt acatgtcggt ggtcacgtgc agaccttccg cgcgcgcctt gtcgatgcgt
     8161 gcgatgacct ggtccatctt gtcccagttc gatttgcccg cggccttgag gtggtagatc
     8221 tcgacgggta tctgcgcgcg ccgcgcgatg tcgatcgttt cgtccaccgc ttccagcagg
     8281 cgctcgcctt cgctgcgcat gtgcgcgatg taggag
//
LOCUS       DASQJS010000212         4713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2318595, whole genome shotgun sequence.
ACCESSION   DASQJS010000212 DASQJS010000000
VERSION     DASQJS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2318595"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VEW08_08630"
     CDS             <1..531
                     /locus_tag="VEW08_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40841.1"
                     /translation="AIAQARIPVGFGASLTIGGLIALAVVGVAMRVAHSSEFGRTVYA
                     IGGGESSARLMGLSVDSTKIGVYLLSGACAALGGIVMTFYMLSGYSLHAVGLELDAIA
                     AVVIGGTLLAGGVGYVAGTLFGVLILGIIQTLIAFDGSLSSWWTRIVVGALLLLFCLL
                     QRLFEGRTPKSAQGTA"
     gene            528..1535
                     /locus_tag="VEW08_08635"
     CDS             528..1535
                     /locus_tag="VEW08_08635"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HYJ40842.1"
                     /translation="MIRRFGRLPDGQDVEVATLGSADGLQAEILTLGGTLRSVTLPVR
                     GKRIPLVLSFNELDAYLSDTSYVGQLVGRYGNRIAGASFELDGKQYKLTANNPPNTLH
                     GGEIGFGKYLWKVLGSGGGKNTYVKLGHRSPAGINGFPGNLDVTALISVFENTLTLVY
                     EATTDAPTPISLTWHPYFTLSGDPRDKIDDQRLTIAASHYLPVNESRVPTGEIAPVTG
                     TPFDFRKPKVMRVPPPSSHPQIALTSGYDHCWVLDKDAPVAAELYSSASGVRMRVKTN
                     LPGLQVYDASHFRTVYPKWHGICLEPENFPDAPNHANFPSSILRPGETHRSFMSFTFS
                     E"
     gene            1540..1890
                     /locus_tag="VEW08_08640"
     CDS             1540..1890
                     /locus_tag="VEW08_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40843.1"
                     /translation="MKTPLLVLALLFAAQNADAACSEKRSRDASFVELAVPEDAIRPT
                     GVADFSFITDETTVDALIARVGPPDASQGTRTIRLIWCFADSTELWVETPDRVVIESV
                     RHGGKSIFKRAKKK"
     gene            1894..2793
                     /locus_tag="VEW08_08645"
     CDS             1894..2793
                     /locus_tag="VEW08_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40844.1"
                     /translation="MKAAYLHPKYWPTWLGIGALRIFEPLPHRLLYLLGRGLGLLVFV
                     FPTPFKKIARRNLELALPELVPAARDRILREHFAGLGCALFETAMSWWSSNERIRHIT
                     LMTGLENLKAAQATGRGVLLLSAHFNSIEIGCRALAARMPLNVMYRPTKNLLVGEFLH
                     SRRAVQTRRAIPRDDVRTLIKALKDGDVVWYAPDQSFRKKGAEMVKLFGIPAATNTAT
                     SRIAGMTHALVLPYFFERLPGGGYRGTIHPALENFPTDDPVADTERFNRIVEAEVRRM
                     PDQYLWIHRRFKGLTPDYPKYYD"
     gene            2894..3277
                     /locus_tag="VEW08_08650"
     CDS             2894..3277
                     /locus_tag="VEW08_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40845.1"
                     /translation="MKQFYYAIVLCSGLVSAQASAECTYPTAPGAFPDGTKATKEELI
                     AARKIFVQYDGEMTAYIECIVAEYQAELAAKPDLTPKQKKELARVNDEKQTSAIAEIE
                     AVKVRFEKERKAYVELHPPAPKPAN"
     gene            3298..4494
                     /locus_tag="VEW08_08655"
     CDS             3298..4494
                     /locus_tag="VEW08_08655"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HYJ40846.1"
                     /translation="MLTPAQAETLIEQHLTCLPIESLPLTQSAGAVLRENIYAERDHP
                     PFDRVAMDGIALSASAAAAHSGRLRVSGTQAAGDPPQTLADGGSCIEIMTGAMLPLGC
                     DAVVPVELVQRNGDIAEIGRKPVQPWQNVHRRGSDCPQGSLLLSAGTRLSAPEVAIAA
                     GAGMARLRVSAQPMIVVISTGNELVEPGEVIEPHQVRRSNAYGITASLRQHGFARVAD
                     DHVRDDEAHLTERLSFHLRTHDVLILSGGVSMGKLDLVPKVLEKLGVSLVFHHVAQRP
                     GKPMWFGVSQTGPAVFALPGNPVSTLVCLSRFVLPALRAAMGQSPAENPRISLTAPVE
                     VKAPLAFYMPVKLKSDDWGRTSAAPCPTNGSGDFTALAGTDGFVELPPGPNTFPKGFV
                     ARFYRW"
     gene            4502..>4713
                     /locus_tag="VEW08_08660"
     CDS             4502..>4713
                     /locus_tag="VEW08_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40847.1"
                     /translation="METGEKVIALTPPGQNRFPVDALRRPIHDLRISVMDRCNFRCPY
                     CMPRETFHESYRFLKSNERLDFAELLR"
BASE COUNT          849 a         1577 c         1435 g          852 t
ORIGIN      
        1 gccatcgccc aggcgcgtat tcccgtgggc ttcggggcat cgctcaccat cggcggcctc
       61 atcgcgctgg ccgtggtcgg agtcgcgatg cgcgtcgcgc actcgagcga gttcggccgc
      121 acggtgtacg ccattggcgg cggtgaatcc tcggcgcgcc tcatgggact ttcggtcgat
      181 tccaccaaga tcggcgtcta tctactgagc ggcgcatgtg cggcgctcgg cggcatcgtc
      241 atgacgttct acatgctctc gggctacagc ctgcacgcag tgggcctgga gctcgacgcc
      301 attgccgccg tggtcatcgg cggcacgctg ctggccggcg gcgtgggcta cgtggccggc
      361 acgttgttcg gcgtgctgat tctcggcatc atccagaccc tcatcgcatt cgacgggagt
      421 ttgagctcat ggtggaccag gatcgtcgtc ggagctttgc tcttgctctt ctgcctgttg
      481 cagcggcttt tcgaggggcg cacgcccaag tcggcgcaag ggacggcatg attcgcaggt
      541 ttggccggct tcccgatggc caggatgtcg aggttgccac gctcggcagc gcggacggcc
      601 tccaggctga aatcctgacg ctcggcggaa cgttgcgaag cgtgaccctc cccgtaagag
      661 gcaagcgcat cccgctggtg ctgtcgttca atgagctgga tgcgtatctc agcgacactt
      721 cttatgtggg gcagctcgta ggtcgctacg gcaaccggat cgcgggcgcc tccttcgaac
      781 tcgatggcaa gcaatacaag ctcaccgcca acaatccgcc caacacgctg cacggcggcg
      841 agatcggctt cggcaagtac ctgtggaagg tgctgggcag cggcggcgga aaaaacacct
      901 acgtgaagct cggccatcgt tcacccgccg gcatcaacgg ctttccaggc aacctcgacg
      961 tcaccgcgct catcagcgta ttcgagaaca cattgacgct cgtgtacgaa gcgaccaccg
     1021 acgcgccgac accgatcagc ctgacctggc atccgtactt cacgctgtcg ggtgatccgc
     1081 gcgacaagat cgacgatcag cggctcacga ttgccgccag ccactatcta cccgtcaacg
     1141 agtcgcgcgt gccgaccggt gaaatcgcgc ctgtgaccgg caccccattc gacttccgca
     1201 agccgaaagt catgcgcgtg ccgccaccct cctcgcatcc gcagatcgcg ctcaccagcg
     1261 gctacgatca ttgctgggta ttggataagg acgcaccagt agccgcagaa ctgtattcaa
     1321 gcgcgagcgg cgtgcgcatg cgcgtcaaaa cgaacctgcc cgggctgcag gtatacgatg
     1381 cctctcactt ccgcaccgtg tacccgaaat ggcacggcat ctgcctcgag cccgagaatt
     1441 tcccggatgc gcccaatcac gccaacttcc cgagcagcat tctccggcct ggcgagacgc
     1501 atcgctcgtt catgagtttc acgttctcgg agtgaccgca tgaagactcc actgctggtc
     1561 cttgccttgt tgtttgccgc gcagaatgcc gacgctgcat gcagcgagaa gcgctcgcgc
     1621 gatgcttcgt tcgtcgaact tgccgtgcct gaggacgcga tccggcccac gggtgtcgcg
     1681 gacttcagtt tcatcaccga cgagaccacg gtcgatgcgc tcattgccag ggtcgggccg
     1741 cccgacgcct cgcagggcac gcgcaccatc cggctcatct ggtgtttcgc ggattcgacg
     1801 gaactgtggg tggagacgcc cgaccgcgtc gtcatcgagt ccgtacgcca cggcggaaaa
     1861 tcgatcttca agcgcgcgaa gaaaaaatag cgcgtgaaag ccgcctacct ccatcccaag
     1921 tactggccta cctggctcgg catcggcgcg ttgcgcatct tcgagccgct gccccaccgt
     1981 cttctctatc tactcggccg gggactgggg ctgctcgtgt tcgtgttccc gacgccgttc
     2041 aagaagatcg cgcggcgcaa cctcgagctc gcgttgcccg agctggtgcc cgccgcgcgt
     2101 gaccgcatcc tgcgcgagca tttcgcgggc ctgggctgcg cgctgttcga gacggcgatg
     2161 agctggtggt cctcgaacga acgcatccgg cacatcacgc tgatgaccgg gctcgagaac
     2221 ctcaaggcgg cacaggcaac cggacgcggc gtactgctgc tgtcggctca cttcaactcc
     2281 atcgaaatcg gctgccgcgc gctggcggcg cgtatgccgc tcaacgtcat gtaccgcccc
     2341 accaagaatc tgctcgtagg tgaattcctg cattcgcgcc gcgcggtgca gaccaggcgc
     2401 gcgattccgc gcgacgacgt gcgcacactc atcaaagcgc tgaaggacgg cgacgtggtc
     2461 tggtatgcgc cggaccagag cttccgcaag aaaggcgccg agatggtgaa gttgttcggc
     2521 attcccgcgg ccaccaacac cgctacctcg cgcatcgccg gcatgaccca cgcgctggta
     2581 ttgccctatt tcttcgagcg gcttccgggc ggcggttatc gcggcaccat ccaccccgcg
     2641 ctcgagaact tccccaccga cgacccggtg gccgacaccg agcggttcaa ccgcatcgtc
     2701 gaggccgagg tgcgccgcat gcccgaccaa tacctgtgga ttcatcggcg attcaagggt
     2761 ttgaccccgg attacccgaa gtattacgac tagggggtac ggaccaaggg ctgacccctt
     2821 tttctgggaa aagggggctg cccccctcta agatagccac cctttccaga accattacgc
     2881 ggggattcga ttcatgaagc agttctacta tgcgatcgtc ctgtgctcgg ggctcgtctc
     2941 agcgcaggcc agcgccgagt gcacctaccc aactgccccc ggcgcttttc cagacgggac
     3001 gaaagcgacc aaggaagaat tgattgccgc ccggaaaatc ttcgtgcagt acgacggcga
     3061 aatgaccgcg tacatcgaat gcatcgtggc cgagtaccag gccgagctgg ccgcgaagcc
     3121 cgatctcact cccaagcaga agaaggaatt ggcacgcgtc aacgacgaaa agcagacctc
     3181 ggccatcgcg gaaatcgaag cggtgaaagt gcgcttcgag aaagagcgca aggcctatgt
     3241 cgagttgcac ccgccggcgc cgaagcccgc caactgagac tcgccggcgc ccgggacatg
     3301 ttgacgccag cccaggccga aacactcatc gagcagcacc tcacgtgcct gcccatcgag
     3361 tcactgccgc tcacacagtc ggcgggagcc gtgctgcgcg aaaacatcta cgccgagcgc
     3421 gatcacccgc cgttcgatcg cgtcgccatg gacggcatcg cgctgtccgc gtccgccgcg
     3481 gcagcgcact ccgggcgcct gcgggtcagc ggcacgcaag ccgccggcga tccaccgcag
     3541 actctggcgg atggcggctc gtgcatcgag atcatgacgg gcgccatgct gccgctcggt
     3601 tgcgatgcgg tcgtgcccgt cgagctggtc cagcgcaatg gcgacatcgc cgagatcggg
     3661 cgcaagcccg tgcaaccgtg gcagaacgtg catcgtcgcg gctccgactg cccgcagggt
     3721 tcgctgctgt tgagcgcggg cacgcgactc tcggcgcccg aggtggccat cgccgccggc
     3781 gccggcatgg cgcgcctgcg agttagcgcc caaccaatga tagtggtgat ctccaccggc
     3841 aatgaattgg tggagcccgg tgaagtcatc gagccgcacc aggtacgccg ctcgaacgcc
     3901 tatggcatca cggccagtct gcgccagcac ggcttcgcgc gtgttgccga cgaccacgtg
     3961 cgcgatgatg aagcgcatct cacggaacga ctgagctttc acctgcgcac acacgacgtg
     4021 ctgatccttt ccggcggcgt gtcgatgggc aaactcgatc tcgtaccgaa agtgctcgag
     4081 aagctcggcg tgagcctcgt gttccatcat gtggcgcaac gcccgggcaa gccgatgtgg
     4141 ttcggcgttt cccagaccgg ccccgcggtg ttcgcattgc cgggcaatcc ggtatcgacg
     4201 ctggtctgcc tgtcgcgatt tgttttgccc gcgctgcgcg ccgccatggg ccagtcgccg
     4261 gccgaaaacc cgcgcatctc gcttaccgca ccggtggaag tcaaggcgcc gctcgccttc
     4321 tatatgccgg tgaaactcaa gagcgacgac tggggtcgca cgtcagcggc tccctgcccc
     4381 accaatggct ccggtgattt cacggcgctc gcaggcaccg atggattcgt cgaacttccg
     4441 cccgggccaa acacgtttcc gaagggattc gtggcgcgct tctatcgttg gtagtaagac
     4501 catggaaacc ggcgagaaag tcattgcgct cacccctccc gggcagaacc ggtttcccgt
     4561 ggacgcgttg cgccggccca tccatgacct gcgcatctcg gtcatggacc gctgcaattt
     4621 ccgttgcccg tattgcatgc cgcgcgagac ctttcacgag tcgtaccggt ttctcaaatc
     4681 gaacgagcgg ctcgacttcg ccgagctgct gcg
//
LOCUS       DASQJS010000213        16509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1688078, whole genome shotgun sequence.
ACCESSION   DASQJS010000213 DASQJS010000000
VERSION     DASQJS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16509
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688078"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1107
                     /locus_tag="VEW08_08665"
     CDS             175..1107
                     /locus_tag="VEW08_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40848.1"
                     /translation="MNNMIWLIRREIWENRSLWIAPLVVAGVILIAAAFGGIHVGNGI
                     NIGWDSSGIDVSEQDRENIRNGLSQALEHKQIIYAITLSSFTAVLLFTMVIVLFFYLL
                     DSLLAERKDRSILFWKSLPVSDAEVVLSKLLTAAVVVPLFVLVVSSALQILFALIWSI
                     RFNGTFLGDALMAWHGPTWLKLQGSFLVVIVTAILWYLPLLAYLMVVSVWARRNAFLW
                     AVLPPVAILTIENMILGSNRFGEFLGHRFIGVFELLESKIGSVHGSGMITVREVLEAV
                     SGVLASHETWLGVLAAAVMIFGAIRIRRFRDDNT"
     gene            1150..2265
                     /locus_tag="VEW08_08670"
     CDS             1150..2265
                     /locus_tag="VEW08_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ40849.1"
                     /translation="MHLTAQRDGNNLDIELAGTWRGTDLPVIDAELAGVSLAGVQALR
                     ITVPESLELDLAGAWTLRQWTKTAEQSGVSVEFAGAVPGQLDLIESTLSGKRQVALAS
                     SSEPTFEPVSALGRIVTRRWLSLQMALDFLGRATLTLFRASTSWRRLRPTSIARHVYE
                     TGLTAIPIASLIAFLITVIIAYIAAQYMRTYGAEIYVVDLITISVLRELAVLLTAIIV
                     AGRSGSAFAAEIGAMKLNEEVDALHAIGVDPHEVLVLPRVIGLVIALPLLTVVADLVG
                     LTGGALLCKWLLDMPLVMFIERAQDSIASTTFWVGIIKAPVFALLIALTGTYRGLQVR
                     DSSRELGRLTTEAVVQSIFLVLFADALFAVAFWLLDI"
     gene            2265..3035
                     /locus_tag="VEW08_08675"
     CDS             2265..3035
                     /locus_tag="VEW08_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ40850.1"
                     /translation="MAEPLIEVLGLVNRFGAQVVHDHLDMTVEQGEVFGIVGGSGSGK
                     SVLLRSILGLQRPQGGEVRMLGRDLTKLSIVELKEIKATYGVTFQQGALFTSLTVLQN
                     VQLPMLEHLHLSPAALEELALLKVRLVGLPDEAARKYPAQLSGGMIKRAALARALALD
                     PRLLMLDEPTAGLDPISAAAFDELLMDLRKQLGLTVIMITHDLDTIFRTCNRVGVIID
                     RKMTSDTLEGITDNPHPWIQAYFHGERALRFGQGKGPE"
     gene            3045..3980
                     /locus_tag="VEW08_08680"
     CDS             3045..3980
                     /locus_tag="VEW08_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HYJ40851.1"
                     /translation="MEREANYTAVGAFVLLIVTMAGLFVYWYAGSGDARDYRRYEIYF
                     EGSVSGLNRGSTVRYLGVEVGRVVSIKIDTRAADRVQVIADIDSHTPISAKTLASLSL
                     QGVTGLLYIDLLANGDTSRRQASVPSEQYPVIDSVQSNFDRLLASLPDLVGRATEVVD
                     RASRVLSDDNIMAFSKTMQNIEMSSASLPGAMRDAAVVLADLKATLVDVRVAAASARQ
                     LMDTSGPDLAAASERFRKISENLASTTANLDRLMTDHREDLGLFLRDSLPEIERLLRD
                     GRSAAQEFGELSRSLKADPSQLLYEPSYKGVEIPR"
     gene            3977..4627
                     /locus_tag="VEW08_08685"
     CDS             3977..4627
                     /locus_tag="VEW08_08685"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HYJ40852.1"
                     /translation="MKLRAWPFLSLSLLLTACSGVFESDLAAPQAYVLRLPAAAAPEP
                     NTSGGTLLVQRPDAGPGLASERIALLRSEHRFDFYAASRWAAPAPDVIESVMIDTLRA
                     TGVFSAVFDDTAPYAPRYNLRCTLRRFEADYTSEGVGKGGGAPAVFVALDCTLGRHRD
                     RALLASFTASGSAAARDDRLSAVVAAFESATAAAMNELGRAASAALASEKPPSEGR"
     gene            complement(4613..6151)
                     /locus_tag="VEW08_08690"
     CDS             complement(4613..6151)
                     /locus_tag="VEW08_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HYJ40853.1"
                     /translation="MAEEPEDIPIDTSPPPPAPRRKRRLGRLFFYGFLSLWALTALWN
                     LFKPLPDGVSVRGTIVETPLDQLKFLNDVTSADVFGVPVVRQQIFDSVIGLIGQSREY
                     LVLDFFLFNGHRGAALDVKPHRELSVELRDALLARKRAMPQMHILVIADPINDVYGGL
                     PSRDLAALRGVGIEVVTPDLDALRDSNPIYSSFWRLTMRWWSGDGSGDAWLPNPLDAG
                     PDQVSLGAWARLLNFKADHRKVIIGDDGKGGIAGIVTSANPHDASSLHSNVALKLSGA
                     ALVPLLESELAIAAEAGWSGGWGATPATSAAALAPENTARVQVLTESEIRAAIVRNFA
                     GARVGDSIDIAMFYLSDRSVIQSLLAAARRGVAVRVLLDPNKDAFGRTKDGIPNRSVA
                     TELAAASDGAIKVRWFRTHGEQFHSKLVAMRTATEFWFTLGSANLTRRNLDDFNLEAN
                     VAASVPLTAEIATQISAWFDSLWTNRGPPELEYTAEFGTYADPSQGSYWLYRLMEATG
                     LSTF"
     gene            complement(6144..8057)
                     /locus_tag="VEW08_08695"
     CDS             complement(6144..8057)
                     /locus_tag="VEW08_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ40854.1"
                     /translation="MLELRELRLRRGAAVLFDRANLTIFRGDKIGIVGRNGCGKSSLL
                     ALLRGDLGADSGDYSVPGKLNFVSVSQELPHTESTVLEYIRGGDSNLMAIEAELEKAR
                     AADDGMQEAHLLGDYDLAGGYTSAARAAELADGLGFAPSDIERRVKELSGGLQMRANM
                     ARALMSRADVLMLDEPTNHLDLDAVLWLEGWLQRFAGTLLLVSHDREFLDDIVNRIVN
                     LESGALTHWTGNYTDFQRQHAASIQQSAAMAARQARETARLHSFIDRFRAQASKARQV
                     QSRIKALSKLETIATYQELDTFEWEFAAANKLPQPLVTLDGASAGYGERVVISGVGRS
                     VNPGERIGILGRNGAGKSTLMKLISGALPPSGGERVASPDLIVGNFAQLEVDRLDARD
                     TALIALTNRIDEIGARSQWDEQRRRNHLGGFGFRGDRVFEPVVHFSGGERARLALAIL
                     VAERPNLLLLDEPTNHLDFEMRHSLITALQDFAGAVIVVSHDRGLLRSVCDQFWLVAD
                     GAVTDFEGDLEDYSNWLEKRGSSASTPAAPKARGAAPRGDKGRGQKREKAEARNDVAP
                     KRQEIKSIETSLEKLGQERARLEKELAGIDFARDAAHARKVTERHGALAREIEQLETR
                     WLELSERLESLNG"
     gene            8156..8929
                     /locus_tag="VEW08_08700"
     CDS             8156..8929
                     /locus_tag="VEW08_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40855.1"
                     /translation="MNRLSCALALAGSWLIAGAALAHAPSSVSVEIVGASGQVFREFP
                     VNARDGALRSYLQAEKGARYQVRVRNTTGQRLGLVIAVDGRNIISGAKSDLARSEPMY
                     VLAPYETQDYAGWRANLDAINEFYFTEWSDSYAEAFGDASARGVIAVAVYGEVAPPPA
                     LRQPYSGNDRSAGAASAPAAKESARRDESAGTGYGDRRVDHAVQVEFVARSNPDSRHF
                     IKYEWRETLCRKHMMDCSEKNRFWDESTLGFAPPPPRRR"
     gene            8958..9590
                     /locus_tag="VEW08_08705"
     CDS             8958..9590
                     /locus_tag="VEW08_08705"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HYJ40856.1"
                     /translation="MKMYRHWALAMVLASPTMVLAAEPTTPPCAASSSAADGMELIDL
                     IDKVSKRTGKQFIVDPRVRAQVPKAGIDLDKVDYARLLAILNVHQFGTYEANGVVRVV
                     PDANARQMPIPVTTDVPAKALDDEYVTVIITAKNMCMAHAVPVLRPLMPQAAHMAADV
                     QSNSLIISDRAANARRIVDMVERLDKAAPAGQKCPPDMWPPAKSDRAEKK"
     gene            complement(9591..12095)
                     /locus_tag="VEW08_08710"
     CDS             complement(9591..12095)
                     /locus_tag="VEW08_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ40857.1"
                     /translation="MTIASFKGKYIAVAVAAAIAVSLAVGGVMVYENSSQHDALFDTA
                     AADTRGRVLGELTLRASELARHLSERVADGVLRDDRDQIRNEIESFRRDETLLGVILR
                     DTAGNELYSWRRPGDNSGGITRSAITPVRANVPAMPGVVAPQTVGELEIEIRSLEGTP
                     ESVAARTNFDSMERKQMRKVLLLAGGLGVIALAVGLALAWWAGRRVHTPITTLIKGAD
                     RIAQGDYSRPMEVGRHDELGELQAALERMRQKLRQTTINKNYLTNVLGSMTDAVFVTS
                     PDGVIRMANQSACKLLAWGEEELVGRSIVSIFDERERGEFDMLRASQDTRETVVKTRQ
                     GQTIPVAITGSQITTDDPQYQGTIFVVRNVTERKRAERRIRYLARYDALTKVPNRMQF
                     QHLLQQAIARAIRNKTSLALLYLDMDRFKEINDTFGHAAGDRTLEILTERLTRALPQE
                     TTIGRLAGDEFAMFVDDLASDADNRGTVAQLARSVLAEIGRAFQLQEHEVFLTASIGV
                     AFCSRDADNVIDLIRNADAAMYHSKQNGGNSFAFYSPEMNAAAVERLMLKSKLRRSLE
                     RDEFLMHYQPKVDLRDGRIVGAEALLRWRLPGHGDIPPSQFIPLAEETNLILEVGEWV
                     LNRVCQDYRDLQQTVTTPGRISLNLSLKQLRQASFILRFRSVFRKHGVSPTCFELEIT
                     ETTLMADSKRTLHLLDELYAMGLHLSIDDFGTGYSSLSALQQFPIGTLKVDQSFVRDV
                     DDDESDATIVRTVIEMGRSLKMNVIAEGVETVGHLHFLRRHHCGFGQGKLFGEPVTLE
                     ELRLLLARQQETGPAFAGLIAQSASLDNKLAKPHSV"
     gene            12238..12963
                     /locus_tag="VEW08_08715"
     CDS             12238..12963
                     /locus_tag="VEW08_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HYJ40858.1"
                     /translation="MTPTPEFSLDQLTGRSRSHLSELANPPCTLHVDAVAPYLAMRAA
                     AAADGLDLTAFSSFRDFDRQLAIWNGKFRGERPMQDRAGLPLDPLSLAPADRVSAILW
                     WSALPGASRHHWGTDFDVVDARAMPAGYKLQVVPAEYHAGGPFHRLTTWLDAHMHAFG
                     FFRPYATDRGGVSPEPWHLSYAPVARRAQTAFSADKLRTVLKDSAIEGKDEVLAALDR
                     NYATYVVNVDAPPEAALLSPQIK"
     gene            complement(12967..13965)
                     /locus_tag="VEW08_08720"
     CDS             complement(12967..13965)
                     /locus_tag="VEW08_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40859.1"
                     /translation="MFAESRRSDYDVTDTVLVSSAPDVRAAIEELFAKVWPGQKFPAF
                     AHVFGDFERMFSGRMPGYFGVDTVYHDQQHTLDITLATARLIVGHEMQVPDGQKFGAE
                     RAMLGIVVALFHDVGYLRTTRDTDVPNGAEFTTVHVSRGAAFLTDYLPKINLGQWTKV
                     AAEIIHYTGYERPFKTIVAPDPRDHKLGHLVGTADLMAQMADRCYLEKCRDRLYAEFV
                     LAGVALPIGENGHVKVRYASGLDLLRQTPKFVFDMRAKRLDGEFNRAYRYLEVLYKGR
                     NPYMESIDRNLEYLQRILRSENWRLLRRQPPVFTGVEDSMATTRTLMLGAIKKVWG"
     gene            complement(14132..14929)
                     /gene="xth"
                     /locus_tag="VEW08_08725"
     CDS             complement(14132..14929)
                     /gene="xth"
                     /locus_tag="VEW08_08725"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HYJ40860.1"
                     /translation="MSAATGSGGTFSIATWNVNSLRVRGEQLLEWLRANSPDVVALQE
                     TKMQDPDFPHAEFTAAGYHSVFSGQKTYNGVALLSRHPMTDVVRDIPDFDDPPRRVLA
                     ATIGDFRVINLYVPNGQAPGSEKFAYKLKWLDALTRWLKIEAEKHPGLVVVGDFNVAP
                     EDRDVHDPAAWVGNVHVSAEERAALGRVTDLGLVDAFRHFEQPEKSFSWWDYRQGAFR
                     RNNGLRIDLILVHRQLLPRLAGCRIDVEPRRVERASDHTPVLAMFSV"
     gene            complement(14926..>16509)
                     /locus_tag="VEW08_08730"
     CDS             complement(14926..>16509)
                     /locus_tag="VEW08_08730"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HYJ40861.1"
                     /translation="RKARYKAVVQELATLAARFEENVLDAMNAWTRLVTDEKELAGIN
                     PVIVEQARVRAVEKGQTGWLFALEQPTYVAVMTDADSEILRRDFYEAWSTRASEVGPT
                     AGKFDNTQVMDRILSLRHEAAQLLDFPNYAAYALATRMAPSNQAVFDFLRELVRVARP
                     AAKREFTELEAFAGRPLNAWDVSYYAEKLQAKLFSISQEELRPYFPLPRVLEGLFSVA
                     EKLFGVKITERQGVPKYHPDARFFEILDANGEVRAGFYLDACARAKKRAGAWMDDCVG
                     RKDIGADHTVPVAYLVCNFLPAAPGKPALLTHDDVVTMFHEFGHGLHHMLTRVGYPSI
                     AGINGVSWDAVELPSQFMENFAWRPEVLAAMARHYETGEPLPAERQKQLLGSRTFHAG
                     LATMRQLEFALFDFRLHAEYDPARGARVHETLSEVRDEVAVFAVPEWNRFPHNFGHIF
                     AGGYAAGYYSYKWAEVLSADAFAAFEEQGVFDKRVAQRFLDSILSQGGSKKALDAFVD
                     FRGRPPDVNALLRQQGLIQ"
BASE COUNT         2826 a         5225 c         5413 g         3045 t
ORIGIN      
        1 tttctgggaa cgcgagatat tcggccgcat cgccatgctc ttcgacggcc gcgaccagca
       61 ggagctcgcc gccctgggtg aagtgcgcgc accgtcggtc gccgacctgt tcgtcgccat
      121 gatgcagccg gtgggcacgg cacagtcacc gtcatcgccg tcgaaggagg ccgcatgaac
      181 aacatgatct ggctcattcg ccgcgaaatc tgggaaaacc gttcgctgtg gatcgcaccg
      241 ctggtggtgg ccggcgtcat cctcattgcc gccgcgtttg gcggcatcca cgtgggtaat
      301 ggcatcaaca tcggctggga ttcctccgga atcgacgttt cagaacagga tcgcgaaaac
      361 atccgcaacg gattgtcgca ggcgctggag cacaagcaga tcatctacgc gatcacgctg
      421 tcctcgttca ccgcagtgct gctgttcacg atggtcatcg tgctgttctt ctatctactc
      481 gactccctgc tggccgagcg caaggaccgc agtatcctgt tctggaagtc gctgccggtg
      541 tccgacgccg aggtggtcct gtccaagctg ctgaccgcgg cggtggtagt gccgctgttc
      601 gtgctggtgg tcagctcggc attgcagatc ctcttcgctc tcatctggtc gatccggttc
      661 aacggtactt ttctgggtga tgcgctgatg gcatggcacg ggcctacctg gctcaagctg
      721 cagggatcgt tcctggtcgt catcgtcacg gcaatcctct ggtacctgcc gctgcttgcc
      781 tacctgatgg tggtctcggt ctgggcgcgc cggaatgctt tcctgtgggc ggtattaccg
      841 ccggtggcaa tcctgacgat cgaaaacatg atcctgggtt cgaaccgatt cggtgaattc
      901 ctcggacacc ggttcatcgg tgtgttcgag ctgcttgaaa gcaagatcgg gagcgtccac
      961 ggcagcggca tgatcacggt gcgcgaagtg ctcgaggcag tttcaggagt actggccagt
     1021 cacgagacct ggctcggtgt gctcgcggcg gccgtgatga tttttggcgc catccgcatc
     1081 cgccgcttcc gcgacgacaa cacctgatcc gccgatgcag gcggcccgcg ggttacactc
     1141 gcggccgcca tgcatctaac cgcgcagcgg gacggcaata acctcgacat cgaactagcc
     1201 ggaacgtggc gcggcacgga cctgcccgtc atcgatgcag aactcgcggg ggtgtcgctc
     1261 gccggtgttc aagcgctgcg aattaccgtt cccgaatcgc tggagctcga cctggcgggg
     1321 gcctggacct tgcgtcaatg gacgaagacc gccgagcaat ccggtgtcag cgtggagttc
     1381 gcgggtgccg ttcccgggca gctggacctg atcgaatcca cgctttccgg caagcggcag
     1441 gtagccctgg catcctcgtc ggaacccacg ttcgaacccg tgagcgcgtt gggccgcatc
     1501 gtgacccggc gctggctcag cctgcagatg gcgctcgact ttctcgggcg cgcgacgctc
     1561 accttgtttc gcgccagcac gagctggcgg cggctgcggc ccacctcgat cgcccggcac
     1621 gtgtacgaaa ccggcctcac cgcgattccc atcgcctcgc tgatcgcttt cctgatcacg
     1681 gtgatcattg cctacatcgc cgcgcagtac atgcgcacct atggcgccga aatctacgtc
     1741 gtcgacctga tcaccatcag tgtgctgcgc gagttggcag tgctgctgac ggccattatc
     1801 gttgctggcc gctcgggcag cgccttcgcg gccgaaatcg gcgcgatgaa actgaacgaa
     1861 gaagtcgacg cgctgcatgc catcggtgtc gatcctcacg aagtgctggt gcttccccgc
     1921 gtcatcggcc tggtcatcgc cttgccgctg ctcaccgtgg ttgcggacct ggtcgggctc
     1981 accggcgggg cgttgctctg caaatggctg ctcgacatgc cgctggtcat gttcatcgag
     2041 cgcgcccagg actcgattgc gtccaccaca ttctgggtgg gcatcatcaa ggccccggta
     2101 tttgcgctgc tgatcgcgct cactggtacc tatcgcggcc tgcaggtgcg cgattcttcg
     2161 cgtgagctcg gtcgcctgac taccgaggcg gtcgtgcagt cgatcttcct cgtgttgttc
     2221 gccgacgcgc tgttcgcggt ggcattctgg ctgctggaca tctgatggcc gaacctctca
     2281 tcgaagtttt gggcctcgtg aaccgcttcg gtgcgcaggt ggtgcatgac cacctcgaca
     2341 tgaccgtgga gcagggcgag gtcttcggca tcgtcggcgg ttcgggttcg ggcaaatcgg
     2401 tgttgctgcg ctcgatcctg ggactgcagc ggcctcaggg cggggaagtg cgcatgctgg
     2461 ggcgggacct cacgaagtta tccatcgtcg agctcaagga gatcaaggcg acctacggcg
     2521 tcacgttcca gcagggcgcg ctgtttacct cgctgaccgt cctgcagaac gttcagttgc
     2581 ccatgctcga gcacctgcac ctgtcgccgg ccgctctcga ggaactggcg ctgctgaaag
     2641 tgcggctcgt gggcctgccc gacgaggcgg cgcgcaagta tcccgcgcag ctctccggcg
     2701 gcatgatcaa gcgcgccgcg ctggcgcgcg cgctggcgct cgatccacgt ctgctgatgc
     2761 tggacgagcc caccgccggt ctcgatccga tcagcgccgc cgctttcgac gagctgctca
     2821 tggacctgcg caaacaactg ggcctcacgg tgatcatgat cacgcacgat ctcgacacca
     2881 tcttcagaac ctgcaatcgt gtcggggtga tcatcgaccg caagatgacg agcgacaccc
     2941 tcgagggcat cacggacaat ccacatccgt ggatccaggc gtatttccac ggagagcgcg
     3001 ccctgcgctt cggccaggga aagggcccag agtaaagaaa aacaatggaa cgggaagcta
     3061 actacactgc agtcggcgcc ttcgtgctgc tcatcgtcac gatggccggc ctgttcgtgt
     3121 actggtacgc gggcagcggc gacgcgcgcg actaccggcg ctacgaaatc tacttcgaag
     3181 gcagcgtgtc gggcctcaat cgcggcagca cggtgcgcta cctcggcgtg gaagtgggcc
     3241 gggtggtgag catcaagatc gatacgcgcg cggccgaccg cgtgcaggtc atcgccgaca
     3301 tcgattcgca cacgccgatc tccgcgaaaa cgctggcgtc gctgtcgctc cagggcgtta
     3361 ccggcctgct gtacatagac ctgctcgcca acggggacac gagccgccgc caggcatctg
     3421 tcccgagcga gcaatatccg gtcatcgatt cggtgcagtc gaatttcgat cgcctgctgg
     3481 cgagtcttcc ggatctcgtc gggcgcgcca cggaagtggt cgatcgcgct tcgcgcgtgc
     3541 tgtccgacga caacatcatg gctttctcga agaccatgca gaacatcgag atgtcttcgg
     3601 cctcgttgcc gggcgccatg cgcgatgcgg cggtcgtgct tgcagacctc aaggccacgc
     3661 tcgtcgacgt tcgtgtggcc gccgccagcg cgcgccagct catggacacc tcgggacctg
     3721 acctggccgc cgcgtccgag cgcttccgca agatttccga gaacctggca agcaccacgg
     3781 ccaacctgga ccgactcatg accgatcatc gagaagacct tggcctcttc ctgcgcgaca
     3841 gcctgccgga aatcgagcgg ctgctgcgcg acggccgcag cgccgcgcag gaattcggcg
     3901 aattgtcgcg cagcctgaag gccgatccgt cccagctgtt gtacgagccg agttacaagg
     3961 gcgtggagat tccgcgatga agctgcgtgc ctggccgttc ctctcgctct cgctgctgct
     4021 caccgcctgc agtggcgtgt tcgaaagcga tctggcggcg ccgcaggcat atgtgttgcg
     4081 cctgcccgcc gccgccgcgc cggagccaaa caccagcggc ggcaccctgc tggtacagcg
     4141 gccagacgcc ggtcccggcc tggcgtccga gcgcattgcg ctgctgcgaa gtgagcaccg
     4201 cttcgatttc tacgccgcca gccgctgggc cgcacccgca ccggatgtca tcgagagcgt
     4261 gatgatcgac accctgcgcg ccaccggcgt tttctccgcg gtgttcgacg acacggcgcc
     4321 gtacgcaccg cgttacaacc tgcgttgcac gctgcgccgc ttcgaagccg actacacgtc
     4381 tgaaggagtt ggaaagggtg gcggtgcacc cgccgtcttc gtcgcgctcg actgcacgct
     4441 cggccgccat cgcgaccgcg cgctgctcgc gagcttcacg gcttcagggt cggcggcagc
     4501 ccgggacgac cggttgagcg cagtggtcgc ggcatttgaa tccgccactg cggcggcgat
     4561 gaacgagctt gggcgcgccg cgtccgcagc gctcgcaagc gaaaagccgc catcagaagg
     4621 tagatagccc ggtggcttcc atcaggcggt agagccagta gctgccctgc gacggatcgg
     4681 cgtaagtgcc gaattccgcc gtgtattcga gctcgggtgg tccgcggttg gtccacaggg
     4741 aatcgaacca ggcgctgatc tgcgtggcga tttcggccgt caatggcaca ctcgcggcga
     4801 cattggcttc gagattgaaa tcatccaggt tgcgccgcgt caggttggcg gaaccgagcg
     4861 tgaaccagaa ctccgttgcg gtacgcatgg cgacgagctt gctgtggaac tgttcgccgt
     4921 gggtacggaa ccagcgcacc ttgattgccc cgtcggatgc cgcggcgagt tcggtggcga
     4981 ccgaacggtt cgggatgccg tccttggtgc ggccgaacgc gtccttgttg ggatcgagca
     5041 gcacgcgcac cgccacgccg cgcctggccg cggccagcag tgactggatc acgctgcgat
     5101 ccgacaggta gaacatcgcg atgtcgatgc tgtcaccgac gcgtgcgccg gcaaaattgc
     5161 gcacgatcgc cgcgcggatt tcgctttcgg tgaggacctg gacgcgcgcc gtgttttccg
     5221 gcgcgagcgc cgcggccgac gttgcgggcg tcgcccccca cccgccgctc cagcccgctt
     5281 cggcggcgat ggcgagctcg ctttcgagca atggcacgag cgccgcaccg gagagcttca
     5341 atgcgacgtt ggagtgcagg ctgctcgcgt cgtgcggatt ggcggaagtg acgatgccgg
     5401 cgatgccgcc tttcccgtcg tcgccgatga tcaccttgcg gtgatccgcc ttgaaattga
     5461 gcaggcgcgc ccatgcgccg agggagacct ggtcgggccc ggcatcgagc gggttcggaa
     5521 gccacgcatc acccgagccg tcgccggacc accagcgcat cgtcagccgc cagaaggatg
     5581 agtagatagg gttggaatcg cgcagggcgt cgaggtcggg cgtcaccacc tctatgccca
     5641 cgccgcgcag cgcggcgaga tcacgcgagg gcaatccgcc gtacacgtcg ttgatgggat
     5701 cggcgatgac caggatgtgc atctgcggca tcgcgcgttt gcgcgccagc aacgcgtcac
     5761 gcaattcgac cgacagctcg cggtgtggct tcacatcgag cgccgcacct cggtggccat
     5821 tgaacaggaa gaagtcgagc accaggtact cgcgcgactg gccgatgagc ccgatcaccg
     5881 aatcgaatat ctgctggcgc acgaccggaa cgccgaagac atcggcgctg gtcacatcgt
     5941 tcaggaactt cagctggtcg agcggggtct cgacgatggt gccgcgaacg ctcacgccat
     6001 cgggaagcgg cttgaacaga ttccagagcg cggtcagcgc ccagaggctc aggaaaccat
     6061 agaaaaacag ccgtcccaag cgccgctttc gtcgcggcgc cggtggcggg ggagacgtgt
     6121 cgatcggtat gtcttcaggc tcttcagcca ttcagcgatt ctagccgctc ggacagctcc
     6181 agccagcgtg tctccagttg ctcgatttcg cgggcgaggg cgccgtggcg ttccgtgacc
     6241 ttgcgtgcat gcgcggcgtc gcgggcgaag tcgatgccgg cgagttcctt ttcgagcctg
     6301 gcgcgttcct ggccgagttt ttccagcgat gtctcgatgg atttgatctc ctgccgcttg
     6361 ggcgcgacgt cgttgcgcgc ctcagccttt tcccgctttt gcccgcgccc cttgtcacca
     6421 cgaggcgccg cgccacgcgc cttgggtgca gcgggcgtgg atgcggagct gccgcgtttt
     6481 tcgagccagt tcgagtagtc ctcgagatct ccctcgaagt cggtgaccgc gccatcggcc
     6541 accagccaga actggtcgca gacgctgcgc agcaggcccc gatcgtgcga gaccacgatc
     6601 accgcgccgg cgaaatcctg caaagcggtg atcagtgaat gccgcatctc gaaatcgaga
     6661 tggttggtgg gctcgtcgag cagcagcagg ttggggcgct cggccacgag gatggcgagc
     6721 gccagccgcg cgcgctcacc gccggagaaa tggaccaccg gttcgaatac ccggtcgcca
     6781 cggaagccga agccaccgag atgattgcgg cgccgttgct catcccattg cgagcgcgca
     6841 ccgatttcgt cgattcggtt ggtcagtgcg atcagtgcgg tgtcgcgggc gtcgagccgg
     6901 tcgacttcga gctgcgcgaa gttgccgacg atgaggtccg gcgaggcgac gcgctcgccg
     6961 ccggagggtg gcagcgcgcc cgagatgagt ttcatgagcg tggacttgcc ggcgccgttg
     7021 cgtccgagaa taccgattcg ttcgccggga ttcaccgagc gccccacgcc cgagatgacc
     7081 acccgttcgc cgtatcccgc cgaagcgccg tccagcgtga ccagcggttg cggcagtttg
     7141 ttagctgccg cgaattccca ctcgaacgtg tcgagctcct ggtaggtggc gatggtctcg
     7201 agcttcgaca acgccttgat gcgactctgc acctggcggg ccttgctggc ctgcgcacgg
     7261 aagcggtcga tgaacgagtg cagccgcgcc gtttcgcgcg cctggcgtgc ggccatcgcc
     7321 gccgactgct ggatgctggc ggcgtgctgg cgttgaaaat ccgtgtagtt gccggtccag
     7381 tgcgtgagcg caccgctctc gagattgacg atgcggttca cgatgtcgtc gaggaactcg
     7441 cggtcatgcg ataccagcag cagcgtgccg gcgaaccgct gcagccagcc ttcgagccac
     7501 aacaccgcat ccaggtccag gtggttagtc ggttcgtcga gcatcagtac gtcggcgcgc
     7561 gacatcagcg cgcgcgccat gttggcacgc atctgcaggc cgcctgacaa ctccttgacc
     7621 ctgcgctcga tgtcggaggg cgcgaaccca aggccgtcgg ccagttccgc ggcgcgcgcg
     7681 gcggaggtgt aaccgccggc gagatcgtag tcgcctaaca ggtgcgcctc ctgcatgccg
     7741 tcatcggcgg cgcgcgcctt ttcgagctcg gcttcgatgg ccatgaggtt cgaatcgccg
     7801 ccgcgtatgt attcgagcac cgtggactcg gtgtgcggca attcctgcga caccgagacg
     7861 aagttgagct tgccgggaac ggaatagtca ccggaatcgg caccgagatc gccacgcaac
     7921 aaagcgagca ggctcgactt gccgcacccg ttgcgcccga caataccgat cttgtcaccg
     7981 cggaagatgg tcaggtttgc gcggtcaaaa aggaccgccg cgcctcgtcg caggcgcagt
     8041 tctcgtagtt caagcatggg cgcggattct actggaatcg gtcgtgctaa ccccggaagg
     8101 agtcatgcca cgttcacgat cgggtacgca cccttgcgtg tggtggagat gtcaaatgaa
     8161 ccgtttgtcg tgtgccctgg ctcttgccgg aagctggctc atcgcgggag cggcgcttgc
     8221 gcacgcgccg tcgtcagtca gcgtggaaat cgtcggagcc tcgggccagg tttttcgcga
     8281 gtttcccgtg aatgcgcgcg atggcgcgct gcgttcctac ctgcaggcgg aaaagggcgc
     8341 gcgatatcag gtgcgggtgc gaaacaccac cggccagcgg ctgggactgg tcatcgcggt
     8401 ggacggccgg aacatcatca gcggcgccaa gtcggacctg gcgcgttccg aacccatgta
     8461 cgtgctcgcg ccctacgaaa cccaggatta cgccggctgg cgcgccaacc tggacgcgat
     8521 caacgagttc tatttcaccg agtggagcga ttcgtacgcc gaagccttcg gcgatgcttc
     8581 cgcgcgtggc gtcatcgccg tcgcggtgta cggcgaggtg gcgccgccgc cggcattgcg
     8641 gcaaccctat tccggcaatg atcgcagcgc cggcgcggca agcgctcccg cagcgaaaga
     8701 atcagcacgc cgcgacgaat cggccggcac gggttatggg gaccggcgcg tcgatcacgc
     8761 cgtgcaagtc gaattcgtcg cgcggtcaaa ccccgacagc cggcacttca tcaaatacga
     8821 gtggcgcgag acgctgtgcc gcaagcacat gatggattgc agtgagaaga accggttctg
     8881 ggatgaatca acgttgggct ttgcgccgcc gcccccgcgc cggcgctgac cctgatcaac
     8941 caagggagaa gatagaaatg aaaatgtatc gccattgggc gctggcaatg gtgctggcct
     9001 cacccaccat ggtgttggcc gccgagccaa ctactccgcc atgtgccgcg tcttccagcg
     9061 cggcggatgg aatggagctg atcgacctca tcgacaaagt ttcgaagcgc accggcaagc
     9121 agttcatcgt cgatccgcga gtacgcgccc aggttccgaa agccgggatc gatctcgaca
     9181 aggtggacta cgcaagattg ctcgccatcc tgaatgtcca ccagttcggc acctatgaag
     9241 caaatggagt cgtgagagtg gtgcccgacg cgaacgcgcg ccagatgccg attccggtca
     9301 cgaccgatgt gccggcgaaa gcgctcgatg acgagtacgt aacggtaatc atcactgcga
     9361 aaaacatgtg catggcgcat gcggtgccgg tgctgcgtcc gctcatgcca caggccgccc
     9421 acatggcggc ggacgtacag agcaactcgc tgatcatctc cgatcgtgcg gcaaacgcgc
     9481 gccgcatcgt cgacatggtt gagcggctgg ataaggcggc gcccgcaggc cagaagtgcc
     9541 cgccggatat gtggccgcca gcgaagagcg acagggccga aaagaagtag ttagacgctg
     9601 tgcggcttgg cgagcttgtt gtccaggctc gccgactgcg cgatcaggcc ggcgaaagcc
     9661 gggccggttt cctgctggcg cgccagcagc aggcgcagtt cctcgagcgt gacgggctcg
     9721 ccgaacagct tgccctggcc gaacccgcag tgatggcggc gcaggaagtg caggtggccc
     9781 acggtctcca cgccctcggc gatcacgttc atcttgaggc tgcggcccat ctcgatgacg
     9841 gtacgcacga tggtggcgtc gctctcgtca tcgtccacgt cgcgcacgaa cgactggtcg
     9901 accttgaggg tgccgatggg gaactgctgc agcgctgaca acgaggaata accggtgccg
     9961 aagtcgtcga tactgaggtg caggcccatc gcataaagct cgtccaacag gtgcagcgtg
    10021 cgcttggaat cggccatcag cgtggtttcg gtgatttcga gttcgaagca cgtgggcgag
    10081 acgccgtgct ttcggaacac cgaccggaaa cgaaggatga agctcgcctg gcgaagctgc
    10141 ttgagcgaaa gattgagcga gatgcggccc ggcgtggtga ccgtctgctg caggtcgcgg
    10201 taatcctggc agacgcgatt cagcacccac tcgccgactt ccaggatcag attggtttct
    10261 tcggccagcg gaatgaactg cgagggcggg atgtcgccat gtcccggcag gcgccagcgc
    10321 aacagcgcct cggcgccgac gatgcgcccg tcgcgcaggt ccaccttggg ctggtagtgc
    10381 atgaggaatt cgtcgcgctc gagtgagcgg cgcaacttgc tcttcagcat cagccgctcg
    10441 accgcggcgg cgttcatttc cggcgaatag aacgcgaacg aatttccgcc gttctgcttg
    10501 gagtggtaca tggccgcatc cgcgttgcgg atgaggtcga tgacgttgtc agcgtcgcgc
    10561 gagcagaaag ccacgccgat gctcgcggtg aggaacacct cgtgctcctg cagctggaac
    10621 gcgcggccga tctcggccag tacgctgcgc gcgagctgcg cgaccgtgcc gcggttgtcg
    10681 gcatcggagg cgaggtcgtc cacgaacatc gcgaactcat cgcccgcgag gcggccgatg
    10741 gtggtttcct gtggcagcgc gcgcgtcagg cgctcggtca ggatctccag cgtgcgatcg
    10801 ccggccgcgt ggccgaacgt gtcgttgatc tccttgaacc ggtccatgtc gaggtagagc
    10861 aacgcgaggc tggtcttgtt gcggatggcg cgtgcaatcg cctgctgcag caggtgctgg
    10921 aactgcatgc gattggggac tttggtcagc gcatcgtaac gggcgagata acgaatgcgc
    10981 cgttcggcgc gtttgcgttc ggtgacgttg cggacgacga agatggtgcc ctggtactgc
    11041 ggatcgtcgg tggtgatctg cgaaccggtg atggctaccg gaatcgtctg gccctggcgc
    11101 gtcttcacca cggtttcgcg cgtgtcctgc gaagcgcgca gcatgtcgaa ttcgccgcgc
    11161 tcgcgttcat cgaagatcga cacgatgctg cggccgacca gttcttcttc accccaggcg
    11221 agcagcttgc acgccgactg gttggccatg cggatcacgc cgtcgggcga cgtgacgaac
    11281 acggcatcgg tcatgctgcc cagcacgttg gtcaggtagt tcttgttgat ggtcgtctgg
    11341 cgcagctttt gccgcatgcg ttcgagcgct gcctgcagct cgcccagctc atcgtgccgc
    11401 ccgacttcca tcggccgcga atagtcgcct tgtgcaatgc ggtcggcgcc cttgatgagc
    11461 gtggtgatgg gcgtgtgaac gcggcggccg gcccaccagg cgagcgccag gcccaccgcc
    11521 agcgcgatga cgccgaggcc acccgccagc agcagcacct tgcgcatctg cttgcgctcc
    11581 atcgaatcaa aatttgttcg cgcggctacg gattccggcg tgccttcgag ggagcggatc
    11641 tcgatctcga gttcgccgac cgtttgcgga gcgacgacgc caggcatggc cggcacattg
    11701 gcgcgcaccg gcgtgatcgc gcttcgcgta atgccgccgc tgttgtcgcc cgggcggcgc
    11761 cacgagtaga gctcatttcc cgcggtgtcg cgcaggatca cgcccagcag tgtttcatcg
    11821 cgcctgaagg attcgatttc gttcctgatc tggtcgcgat cgtcgcgcag gactccgtca
    11881 gcgactcgtt cggacaggtg acgtgcaagc tcgctggccc gcaaggtgag ctcaccgagc
    11941 acccggccgc gggtatcggc ggccgctgta tcgaacagcg cgtcgtgctg cgagctgttc
    12001 tcgtagacca tcacaccgcc cacggccaac gacaccgcga tggcggctgc cacggcgacg
    12061 gcgatgtact tgcccttgaa gctggcgata gtcacgtaat cccgatactt cattatttgc
    12121 gcgcaatctt aaccgcggct ttccgggagc agccaccttc gccggggccc gaatggggcg
    12181 caatgtcgat ttgttccgcc gaaagacggg ttccagcgtc cggtaggata gtcaggaatg
    12241 acgccaaccc cggaattctc gctggatcag ctcactggac gttcccggag ccacctgtct
    12301 gagctggcca accccccatg caccttgcat gtcgacgccg tggcgcccta tctggcgatg
    12361 cgggcggcgg cggcggccga cggtctcgac ctcacggcgt tctcgagttt ccgggacttc
    12421 gaccgccaac tcgccatctg gaacggcaag tttcggggcg agcgccccat gcaggaccgc
    12481 gcgggcctgc cgctggatcc cttgtcgctg gcacccgcgg accgggtcag cgccatcctc
    12541 tggtggtcag ccctgccggg cgcgagccgg caccattggg gcacggattt cgacgttgtg
    12601 gatgcgcggg ccatgccggc cggatacaag ttgcaggttg tgccggccga gtatcacgcc
    12661 ggcggaccat tccatcgcct gaccacctgg ctggatgcgc acatgcacgc tttcgggttc
    12721 ttccgcccgt acgcaactga tcgtggtggc gtgtctccgg agccctggca cctgtcgtat
    12781 gcgcccgtcg cgagacgcgc ccaaacagcg ttttccgcgg acaagctgcg caccgtgctg
    12841 aaggacagcg caattgaagg aaaggatgaa gtgctcgcgg cgctcgatcg caattacgcg
    12901 acctacgtgg tgaacgtgga cgcgccgccg gaggcggccc tgctgagtcc tcaaatcaaa
    12961 tgaggttcag ccccagactt tcttgatggc gcccagcatg agtgtgcgcg tggtggccat
    13021 ggagtcttcg acgccggtga agaccggcgg ctgacgccgc agcagccgcc agttttcgct
    13081 gcgcaggatg cgctgcagat actcgaggtt gcggtcgatg gattccatgt acgggttgcg
    13141 acccttgtac agaacttcca ggtagcgata cgcacggttg aactcgccgt cgagccgctt
    13201 ggcgcgcatg tcgaacacga atttcggagt ctggcgcagc aggtcgaggc ccgaggcgta
    13261 acgcactttc acgtgaccat tctcgccgat cggcagggcg acgccggcga gcacgaactc
    13321 cgcgtacagg cgatcgcggc acttttcgag ataacagcga tcggccatct gcgccatgag
    13381 atcggcggtg ccgaccaggt gcccgagctt gtggtcgcgc gggtcgggcg caacgatggt
    13441 cttgaacggg cgctcgtagc cggtgtagtg gatgatctcg gctgcgacct tggtccactg
    13501 cccgaggttg atcttgggta gatagtccgt gaggaacgcc gcgccgcgcg agacgtgtac
    13561 ggtggtgaac tcggcgccgt tcggcacgtc ggtgtcgcgc gtggtgcgca gatagccgac
    13621 gtcgtggaac agggccacca cgatgccgag catggcgcgc tcggcgccga acttctgccc
    13681 gtccggcacc tgcatttcgt ggccgacgat gagccgcgcc gtggcgagcg tgatgtcgag
    13741 cgtgtgctgc tggtcgtgat agacggtgtc gacgccgaag tacccgggca tgcgcccgga
    13801 gaacatgcgt tcgaagtcac cgaacacgtg ggcgaacgcg gggaatttct ggcccggcca
    13861 gactttggcg aacagctcct cgatggccgc acgaacgtcg ggagccgagc tcacgagaac
    13921 cgtgtcggtt acgtcgtagt cgctgcggcg ggattctgcg aacacgtccg gccttggcct
    13981 tcgagcacta acaagatggg cgagtctagg cagggggtct gcaaaagacc agcgggcgga
    14041 atctgactct gaagcgagcg tgacgtgcat cacggtgggg tgaacccgca atcgctgtat
    14101 tcgcccgctt actgtaaagc gccggttttc tttacaccga aaacatcgcc agaacgggag
    14161 tgtgatccga ggcccgctcg acgcgccgcg gctcgacgtc gatgcgacag ccggcaagcc
    14221 tgggcagcaa ctggcgatgc accaggatga ggtcgatgcg caggccgttg ttccggcgga
    14281 atgcgccctg gcgatagtcc caccagctga acgacttctc cggttgctcg aagtgccgga
    14341 aggcatccac caggccaagg tcggtgacgc ggcctagtgc cgcgcgttcc tcggcactca
    14401 cgtgcacgtt gcccacccac gccgccggat cgtgcacgtc acggtcttcg ggcgcgacgt
    14461 tgaagtcgcc gaccacgacc agcccagggt gcttctccgc ctcgatcttg agccagcgcg
    14521 tgagcgcgtc gagccacttg agcttgtagg caaatttttc cgaacccggc gcctggccgt
    14581 tcggcacgta cagattgatg acgcggaagt cgccaatggt ggcggccagc acccggcgcg
    14641 gcggatcgtc gaagtccggg atgtcgcgca ccacgtccgt catcggatga cgcgacagca
    14701 gcgctacgcc gttgtaggtt ttctgaccgc tgaagacgct gtggtatccc gccgccgtga
    14761 attcggcatg cgggaaatcc gggtcctgca tcttggtttc ctgcagcgcc acaacgtccg
    14821 gagaattcgc gcgcaaccat tcgagcagct gctcaccgcg tactcgcaac gagttcacgt
    14881 tccaggtcgc gatggaaaag gtgccaccgc tgccggtggc agcgctcatt ggatcagacc
    14941 ctgctggcgc agcagcgcat tcacgtccgg cggccggccg cgaaaatcga cgaacgcatc
    15001 gagcgccttt ttcgatccgc cctgcgacag gatcgaatcg aggaagcgct gcgcgacccg
    15061 cttgtcgaac acgccttgct cttcgaacgc cgcgaacgcg tcggcactga gcacttcggc
    15121 ccatttgtag ctgtagtagc ccgccgcgta gccgcccgcg aagatgtggc cgaagttgtg
    15181 cggaaagcga ttccattccg gcaccgcgaa caccgcgacc tcgtcgcgca cctcggaaag
    15241 ggtttcgtgc acgcgcgcac cacgggcggg gtcgtattcc gcatgcaggc ggaaatcgaa
    15301 cagcgcgaac tcgagctggc gcatggtcgc gaggccggca tggaacgtgc gcgagcctaa
    15361 caactgcttc tgccgttcgg cggggagggg ttcgccggtt tcgtagtggc gcgccatcgc
    15421 ggcgagcact tcggggcgcc acgcaaaatt ttccatgaac tggctcggca gttcgactgc
    15481 gtcccacgag acgccgttga tgccggcgat gctggggtat cccacgcgcg tcagcatgtg
    15541 gtgcaggccg tggccgaatt cgtggaacat ggtcacgacg tcgtcgtgcg tgagcagcgc
    15601 cggcttgccg ggcgcggcgg gcaggaaatt gcataccaga taggccaccg gcacggtgtg
    15661 gtcggcgccg atgtccttgc ggccgacgca gtcgtccatc cacgcccccg cgcgtttctt
    15721 ggcgcgtgcg caggcgtcga ggtaaaagcc ggcgcgcacc tcaccattcg cgtcgagaat
    15781 ctcgaagaaa cgcgcatcgg ggtgatattt gggcacaccc tggcgttcgg tgatcttcac
    15841 gccgaacagt ttttcggcca ccgagaacag gccttcgagc acccgcggca gcgggaagta
    15901 cggacggagc tcttcctgcg agatggagaa gagcttcgct tgcagcttct cggcgtagta
    15961 gctcacgtcc catgcattca gggggcggcc cgcaaatgcc tcgagctctg tgaactcgcg
    16021 cttcgcagcc ggccgcgcga cgcgcacgag ctcgcgaagg aaatcgaata ccgcctggtt
    16081 cgaaggcgcc atgcgcgtcg ccagcgcata ggcggcgtag ttggggaaat cgagcagctg
    16141 ggccgcttcg tgccgcagtg agaggatgcg gtccatgacc tgtgtgttgt cgaacttgcc
    16201 ggccgtgggg ccgacttcgg acgcgcgcgt agaccacgct tcgtagaaat cgcgacgcag
    16261 gatttcggag tccgcgtcgg tcatcacggc cacgtaggtg ggctgctcca gcgcgaacag
    16321 ccatccggtc tggccttttt cgacggcacg tacgcgcgcc tgttcgacga tgaccgggtt
    16381 gatgccggcg agttctttct cgtcggtgac caggcgcgtc cacgcattca tggcgtcgag
    16441 cacgttttct tcgaacctgg cggcgagcgt ggccagctcc tgcaccacgg ccttgtatct
    16501 ggccttgcg
//
LOCUS       DASQJS010000214         9912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1373266, whole genome shotgun sequence.
ACCESSION   DASQJS010000214 DASQJS010000000
VERSION     DASQJS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373266"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /gene="rbfA"
                     /locus_tag="VEW08_08735"
     CDS             <1..432
                     /gene="rbfA"
                     /locus_tag="VEW08_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HYJ40862.1"
                     /translation="LLAASSEAVESDERPKLSGKAQRLGRIEAEMQRVLSTLVSREVR
                     DPRVGNITITTVSVAPDMSTARIFFVPFASKHTPEEVMEGLNRAAGFLRGALGRALSL
                     RHAPKLEFIYDLQIENADKLTRLIDGAVKSDKHEPDPDSER"
     gene            complement(566..949)
                     /gene="rbfA"
                     /locus_tag="VEW08_08740"
     CDS             complement(566..949)
                     /gene="rbfA"
                     /locus_tag="VEW08_08740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HYJ40863.1"
                     /translation="MSGKAQRLGRIEAEMQRVLSTLVSREVRDPRVGNVTITTVSVAP
                     DMSTARIFFVPFASKHTPEEVTEGLNRASGFLRGALGRALSLRHAPKLEFVYDQQIEN
                     ADKLTRLIDGAVKSDRHEPDPDPEA"
     gene            complement(978..3524)
                     /gene="infB"
                     /locus_tag="VEW08_08745"
     CDS             complement(978..3524)
                     /gene="infB"
                     /locus_tag="VEW08_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HYJ40864.1"
                     /translation="MSDVTVAQFAEVLKVPVDRLLVQLESAGIQVAGPQALISDEAKL
                     ELLTHLRRAHGADEPSKTAGAAAPRKITLARKSQSELKQASTQGRARTVNVEVRHKKT
                     YIKRDVLEEQNKQTFGAAEEKRKEQEALEQAERDRLEAARKESERIEAENRRRIEDEA
                     TKKKQAEEARRAQEEARAKQETEAQRRAAAEVATAPAARPERHKPEKAPVAAADPAKD
                     TRYGRQELHVAGGVGARNKKKKRVKERTGAGSVGGESRHAFEMPTAPIKREVTIGETI
                     TPQEIAQKMAVKATEVIKTMLNMGVMATINQPIDRDTATLVVEEMGHIAKVLKGDQVE
                     EDLQGEEAPETESPRPPVVTVMGHVDHGKTLLLDYIRTTKVAAGEAGGITQHIGAYHV
                     ETAKGMVTFLDTPGHAAFTAMRARGAKATDVVVLVVAADDGVMPQTIEAIQHAKAAGV
                     PIVVAVNKIDKPDAEPDRVRTELSKHDVIAEEWGGTNMFVQVSAKTGQGVDALLDAIL
                     LQAEVLELTAAADGLATGIVIESSVEKGRGAVATVLVKRGTLKLGDPIIAGNEFGRVR
                     AMFDENGKPVESAAPSIPVQVLGLQGSPNAGDEFLAVENERKAREVALYRQGKFRDVK
                     LAKQASKMGDVFAQMEEQKLGIIPILLKTDVQGSAEALRDALIKLATPEVQVKIIASG
                     VGGITISDVQLAAASKARIVGFNVRADAAARDAMKDNEVEVQYFSIIYEAIDYVKQLL
                     SGLLAPEIKETILGTAQVREVFRSSKFGVVAGCLVTDGVVKRNNPIRVLRDSVVIFEG
                     ALESLRRFKDDAGEVRAGTECGIGVKNYQDVRVGDQIECYSRVEVARSIQ"
     gene            complement(3534..5033)
                     /gene="nusA"
                     /locus_tag="VEW08_08750"
     CDS             complement(3534..5033)
                     /gene="nusA"
                     /locus_tag="VEW08_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003235027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HYJ40865.1"
                     /translation="MNKEILLVVDAVSNEKSVDKEIIFDALEAALASATRKKHGEEWD
                     VRVAINRKTGDYDTFRRWKVFADDSREMEVPQRELRMEDALDINKEAEVGGFVEEPME
                     SVAFGRIAAQQAKQVIVQKVREAERSQVVDAYKDRVGTLVSGVVKRVDRNGIYVDLGG
                     NAEGFVPRTDMIPREIVKSQDRIKAFLKEVRSEPRGPQLFLTRTAREFLIELFKLEVP
                     EVGQGLIQILGAARDPGVRAKIAVKSLEPRVDPVGACVGMRGSRVQAVSNEIAGERVD
                     IIPHDDNAAQFVINSMSPAEVLSIVVDEDAHSMDIAVAEDKLSQAIGRGGQNIRLASQ
                     LTGWDLNVMTETDAEAKSETEAKALVENFKKSLDVDEDVATILVQEGFSTVEEVAYLP
                     QSELANIEEFDDEMVKELRNRARDVLLTQAIASEETLESGMPADDLLLLEGMKPDLAL
                     ALARRGVRTREDLADQAVDDLLDIEGLSSEEAGKLIMKAREIWFEAGRA"
     gene            complement(5048..5503)
                     /gene="rimP"
                     /locus_tag="VEW08_08755"
     CDS             complement(5048..5503)
                     /gene="rimP"
                     /locus_tag="VEW08_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HYJ40866.1"
                     /translation="MDTALHDRLIALFEPVLKEIGYELVEVEFVPGQGGGTLRIYIDH
                     PEGIDVDDCGEASHAVSDLLDANDPFPGAYSLEVSSPGLDRILRTPEHFAQFVDNRVK
                     VELKVARDGRKRYTGMLRRTDGVSIEMDVDNFSVSIKLAEVARARLAPL"
     gene            5737..6060
                     /locus_tag="VEW08_08760"
     CDS             5737..6060
                     /locus_tag="VEW08_08760"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYJ40867.1"
                     /translation="MNISEKLAPIRKGLLEFVILKIVAGDKVYVADMLKLLGPTEFAT
                     QEGTLYPLLSKLRREELVDYEWQESEAGPPRKYYKLTQKGRTQLSTLNDYWKDLNRTL
                     AEFGN"
     gene            6064..7203
                     /locus_tag="VEW08_08765"
     CDS             6064..7203
                     /locus_tag="VEW08_08765"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HYJ40868.1"
                     /translation="MQKVVSISLNGIAYQLEEPGYNQLRSYLERAESRLKDSPDRTEV
                     MNDLEQAIGEKCARVLGPHKTVVNEAEVARIIEEMGPVESAEEKAAGAGAAPEGAPEQ
                     ESAYVGPQPRKRLFNIREGAMWAGVCNGIAAYINVDVTWVRIAFALITIFSWGGMILV
                     YIALAFIVPTAETAEDRAAAFGMPFNTEELIGRAKKNFEKFGSDYRWRREWRRQQRHW
                     NRQWHQMSEQVRQATAQAAPQMGTTARAIMSIFLPIAAIVGAVLFVVWILAMLSLVTQ
                     HTVFGWGLPHGMPLWVGLLVLVLVYFAISAPLKAIRHGGREAAGSHPGWGALHGLMWM
                     GFTVLFFWLAYTFFPGVRELVDQLMWAADLTVSNISETITFGSWD"
     gene            7200..7871
                     /locus_tag="VEW08_08770"
     CDS             7200..7871
                     /locus_tag="VEW08_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ40869.1"
                     /translation="MKSLETVTVETGPNPTFSIIWMHGLGADGHDFEPLVPELLEDGM
                     PVIRFVFPHAPVRPVTINNGYEMRAWYDIIGIDRRSAEDFDGINASAQAIAALVQREN
                     ERGIPTNRIIVAGFSQGGAMALHIATRYPQTFAGVIALSCYLPVARDLPAAHNAANLG
                     TPIFMAHGSQDPVVPIALGEFSREALEAVGYGVEWHTYLMPHSLCPDEVAHLKSWLAR
                     RVAQV"
     gene            complement(7888..8613)
                     /locus_tag="VEW08_08775"
     CDS             complement(7888..8613)
                     /locus_tag="VEW08_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HYJ40870.1"
                     /translation="MKFDDIKKLHEKKFREKFKHFVIEGEHLVQELERAATRDARLRE
                     SKIFVTRDYAHWSSALPMHVIQSKHMAQISETRSPQGIAAVVPLLEPAAPRAGERAVY
                     LHEIQDPGNFGTILRTLAWFGNFRCLCSPDSVDLHNGKVVRASMGAIFHVAVEFDVPM
                     DSLPKRFARIASLDLGGQPVPSPAFREFDCYVFGNEARGLPREKMDALGARPFTIAGT
                     GAIESLNVAATVNICLYELNRTS"
     gene            complement(8610..>9912)
                     /locus_tag="VEW08_08780"
     CDS             complement(8610..>9912)
                     /locus_tag="VEW08_08780"
                     /inference="COORDINATES: protein motif:HMM:NF044849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40871.1"
                     /translation="VVGFGPGGLFAALLLAQMGFKPIVLERGRDVRRRTQDTWGLWRK
                     KVLMPESNVQFGEGGAGLFSDGKLYSQIKDPKFYGRKVMHEFVRAGAPEEILFVSKPH
                     IGTFRLTGIVEKMRAEIIALGGEVRFESKVTDVLIDLGRIAGVVLENGETLSSRYVVL
                     ALGHSSRDTLRMLERRGVFLEAKPFAIGFRIEHPQSLIDRARFGRFAGHPELGAADYK
                     VVHHASNGRSVYSFCMCPGGMVVAATSEAGRVVTNGMSQYSRNERNANAGIVVAINPE
                     PDFPGGPLAGVALQEQLESTAFELGGGDYCAPGQLLGDFVRGKPSTQLGEVQPSYQPG
                     VKLCDLSPSLPAYAIDAIREALPMFGKQIPGFDRDDAVLTSVESRTSSPVRITRNADT
                     LQSLNVRGLYPCGEGAGYAGGILSAGVDGIKVAEAVARDLA"
BASE COUNT         1691 a         3246 c         3050 g         1925 t
ORIGIN      
        1 ttgctcgcag catccagtga ggcggtcgaa tcagatgagc gccctaaatt gagtggaaag
       61 gcacaacgtc tcggccgcat cgaagctgag atgcagcgcg tgttgtcgac tctggtgtcg
      121 cgcgaagtgc gcgacccgcg cgtcggcaac atcacgatca cgacggtgag cgtcgcgccg
      181 gacatgtcca cggcgcgcat cttcttcgtg ccgttcgcga gcaagcacac gcccgaggaa
      241 gtgatggaag ggctgaatcg cgccgcgggt ttcctgcgcg gcgcgctcgg ccgcgcgttg
      301 tccctgcggc acgcgcccaa gctcgaattc atctacgacc tgcagatcga aaacgcggac
      361 aagctcacgc gcctcattga cggcgccgtg aagtcagaca agcacgagcc cgacccagac
      421 tccgaacgat aggcacccca tgcctaccgg catcatcctg ctcgacaaac cccaggggct
      481 aacgtcgaac ggcgcgctgc agcgagccgt tcgacgtgag cccctggggc ttgtcgagca
      541 ggatgatgcc ggtaggcatg ggcgcctaag cttccggatc tgggtcgggt tcgtgcctgt
      601 cggatttcac cgcgccgtcg atgaggcgcg tgagcttgtc ggcgttctcg atctgctggt
      661 cgtaaacgaa ctcgagcttg ggggcgtgcc gcaaggataa cgcgcgtccg agcgcaccgc
      721 gcagaaaccc cgaagcgcgg ttcaatcctt cggtcacttc ttcgggcgtg tgcttgctcg
      781 cgaacggcac gaagaagatg cgcgccgtgg acatgtccgg cgccacgctt accgtcgtga
      841 tggtgacgtt gccgacgcgc gggtcgcgca cttcgcgcga caccagcgtc gacaacacgc
      901 gctgcatctc agcttcgatg cggccgagac gttgtgcctt tccactcaat ttagggcgct
      961 catctgattc gaccgcctca ctggatgctg cgagcaacct cgacgcgcga gtaacactcg
     1021 atctggtcgc cgacgcgcac gtcctggtag ttcttcacgc cgatgccgca ctcggtgccg
     1081 gcacgcactt cgccggcgtc gtccttgaag cgacgcagcg actcgagcgc gccctcgaag
     1141 atcaccacgc tgtcgcgcag cacgcggatc ggattgttgc gcttgaccac gccgtcggtg
     1201 accaggcagc ccgccaccac gccgaacttg ctcgagcgga acacttcgcg cacctgggcg
     1261 gtacccagga tggtttcctt gatctcgggc gcgagcaggc cggagagcaa ctgcttcacg
     1321 tagtcgatgg cttcgtaaat gatgctgaaa tactggactt cgacttcgtt gtccttcatc
     1381 gcatcgcgcg cggccgcgtc ggcacgcacg ttgaagccga cgatgcgcgc cttcgatgcg
     1441 gccgccagtt gcacgtctga aatggtgatg ccgccgacgc cgctggcgat gatcttcacc
     1501 tgcacttcgg gagtggcgag cttgatcaac gcgtcgcgca atgcttccgc gctgccctgc
     1561 acgtcggtct tgagcaggat cgggatgatg ccgagcttct gctcctccat ctgcgcgaac
     1621 acgtcgccca tcttcgaggc ctgtttcgcg agcttcacgt cgcggaactt gccctggcga
     1681 tacagcgcca cttcgcgcgc cttgcgttcg ttttccacgg cgaggaactc gtcgcccgcg
     1741 ttgggcgagc cctgaagacc cagcacctgg acgggaatgg acggcgccgc gctctcaacg
     1801 ggctttccgt tttcgtcgaa catggcgcgc acgcgcccga attcattgcc ggcgatgatc
     1861 gggtcgccga gcttcagtgt gccgcgcttg accagcacgg ttgccaccgc accgcggccc
     1921 ttctcgaccg acgattcgat gacgatgccg gtagcgaggc catcggccgc tgccgtgagc
     1981 tcgagcactt cggcctgcag caggatggcg tccagcaatg catcgacgcc ctggccggtt
     2041 ttcgccgaca cctgcacgaa catgttggta ccgccccatt cttcggcgat gacatcgtgc
     2101 ttcgagagct cggtgcgcac gcgatcgggc tcggcatccg gcttgtcgat cttgtttacg
     2161 gccacgacta tcggaacacc ggcagccttg gcatgttgga ttgcttctat ggtttgcggc
     2221 atgacgccgt cgtcggccgc caccacgagc accacgacgt cagtcgcctt ggcgccgcgc
     2281 gcgcgcatcg cggtgaatgc cgcgtggccc ggcgtatcga ggaaggtgac catgcccttc
     2341 gccgtttcca cgtggtacgc gccgatgtgc tgcgtgattc cgcccgcttc gcccgccgcc
     2401 actttcgtgg tgcggatgta gtcgagcagc aaagtcttgc catggtcgac gtgacccatg
     2461 acggtgacga cgggagggcg cggggattcg gtttccggag cttcctcgcc ctgcagatct
     2521 tcttcgacct ggtcgccttt caggaccttg gcgatgtggc ccatctcttc gaccaccagc
     2581 gtcgcggtgt cgcgatcgat gggctggttg atggtggcca tcacgcccat gttgagcatg
     2641 gtcttgatga cttcggtggc cttgaccgcc atcttctgcg cgatttcctg cggcgtgatg
     2701 gtctcgccga tggtgacttc gcgcttgatc ggagccgtgg gcatttcgaa cgcgtgacgt
     2761 gattcgccgc ctacgctgcc ggcacccgtg cgttccttga cgcgcttttt cttcttgttg
     2821 cgcgcaccga cgccgccggc gacgtgcaat tcctggcgac cgtagcgcgt gtccttggcc
     2881 ggatcggcag ccgcaactgg cgccttctcg ggcttgtggc gctcggggcg tgcagcaggc
     2941 gcagtcgcga cttccgcggc ggcccggcgc tgggcctcgg tttcctgctt ggcgcgcgcc
     3001 tcttcctgcg cacggcgggc ttcttcagcc tgtttcttct tggtggcttc gtcttcgatg
     3061 cgccggcggt tttcagcctc gatgcgctcg ctttccttgc gcgccgcttc caggcggtca
     3121 cgttcggcct gttccagcgc ttcctgttcc ttgcgctttt cctcggccgc gccgaacgtc
     3181 tgcttgttct gctcttcgag aacgtcgcgc ttgatatagg ttttcttgtg gcgaacttcg
     3241 acgttcacgg tgcgcgcacg cccctgcgtg ctcgcctgct tgagctcact ctgtgacttg
     3301 cgcgccagcg tgatcttgcg cggcgcagcc gcgccggcgg tcttgctggg ctcgtcggcg
     3361 ccatgagcgc ggcgcaggtg cgtgagcagc tcgagcttgg cctcgtcgct gatcagcgcc
     3421 tggggccctg cgacctggat tccggcggac tcgagctgca ccagcagccg gtcgaccggc
     3481 accttcagga cctcggcaaa ttgcgcaacg gtgacatcgg acatagattt tccttaggcg
     3541 cgaccggctt cgaaccagat ctcgcgtgcc ttcatgatga gcttgccggc ttcttcggac
     3601 gaaagccctt cgatgtccaa caagtcgtcg accgcctggt cggcgagatc ttcgcgggta
     3661 cgaacgccgc ggcgcgccag tgccagcgcg agatcgggct tcatgccctc gagcagcagc
     3721 aggtcgtcgg ccggcatgcc ggactcgagt gtttcctcgc tcgcgatggc ctgcgtgagc
     3781 agcacgtcgc gcgcgcggtt gcgcagctcc ttgaccattt cgtcgtcgaa ctcttcgatg
     3841 ttggcgagct cggactgcgg caggtaggcc acttcctcga cggtcgagaa gccttcctgc
     3901 accaggatcg tggcgacgtc ctcgtcgaca tccagcgact tcttgaagtt ctcgaccagg
     3961 gccttggctt cggtctcact cttggcttcg gcgtcggtct cggtcatgac attgagatcc
     4021 cagcccgtga gctggctcgc gagccggatg ttctgcccgc cgcgcccgat cgcctgcgag
     4081 agtttgtcct cggccaccgc gatatccatg ctgtgcgcgt cttcgtccac cacgatcgac
     4141 agcacttcgg ccggcgacat ggagttgatg acgaactggg ccgcgttgtc gtcgtgcgga
     4201 atgatgtcta cacgctcgcc ggcgatctcg ttcgagaccg cctgcacgcg cgatccgcgc
     4261 atgccgacgc aggcgccgac cggatcgacg cgcggctcca ggctcttcac ggcgatcttg
     4321 gcgcgcacgc ccggatcgcg ggcggcgccc aggatctgga tgaggccctg gccgacttcc
     4381 ggaacttcga gcttgaagag ttcgatgagg aattcacgcg cggtgcgcgt gaggaacaat
     4441 tgcggaccac gcggctcgga gcgcacttcc ttgagaaacg ccttgatgcg gtcctggctc
     4501 ttgacgatct cgcgcggaat catgtcggtg cgcggcacga aaccttcggc attgccgccg
     4561 aggtccacat aaataccgtt gcgatcgacg cgcttgacca cgcccgaaac gagcgtgccg
     4621 acgcgatcct tgtatgcgtc gaccacctgg gaacgctcgg cctcgcgcac cttctgcacg
     4681 atgacctgct tggcctgctg cgcggcgatg cgtccgaagg ccaccgattc catgggctct
     4741 tcgacgaagc cacccacttc ggcttccttg ttgatgtcca gggcgtcttc catgcgcaac
     4801 tcgcgttgcg gcacttccat ctccctggaa tcgtcggcga agactttcca gcggcggaaa
     4861 gtgtcgtaat caccggtctt gcgattgatg gcgacgcgca cgtcccattc ttcgccgtgc
     4921 ttcttgcgcg tggccgaagc cagcgccgcc tcgagcgcgt cgaagatgat ttccttgtcg
     4981 acgcttttct cgttcgatac cgcgtcgacg accagcaaaa tctctttgtt catgatcttg
     5041 ttcctgacta caacggcgcg agtcgcgccc tagctacctc cgcgagcttt atgctcacag
     5101 agaaattgtc cacatccatc tcgatgctca cgccgtccgt acggcgaagc atgcccgtgt
     5161 atcgcttgcg gccatcgcgc gccaccttga gctcgacctt cacgcggttg tccacgaact
     5221 gcgcgaaatg ctccggcgta cgcaatatgc ggtccagtcc cggcgacgaa acctcgagcg
     5281 aatacgcacc gggaaacgga tcgttggcat cgagcaaatc cgacacggcg tggctcgctt
     5341 ctccgcagtc atcgacgtcg atcccttcgg gatggtcgat ataaatgcgc agcgtcccac
     5401 cgccctgccc gggcacgaac tcgacttcga cgagttcgta tccgatttct ttcagaaccg
     5461 gctcaaaaag ggcgatgaga cggtcatgca acgccgtatc cacacgcccc tccaacaaaa
     5521 aagggcccca aaggccccca aaacacgcga tgcccgtaaa tggcgtcgct ccaaaaaaac
     5581 aaaagcccca tcggggcctt caacatcgcg cgggtcactc gcgggccgct cagcccctac
     5641 ccaatcgagg gcgcggattg tacggcacga aaacggttgt tgacagacca gcggtgactc
     5701 aagtaccttg cggctccaag gtagctgttt gaacccatga acatctctga aaaactcgcg
     5761 ccaatccgaa agggactgct cgaattcgtg atcctcaaga tcgtggccgg ggacaaggtc
     5821 tatgtcgccg acatgctcaa gctcctgggg cccacggaat tcgcgaccca ggaaggcacg
     5881 ttgtatcccc tcctcagcaa gctgcggcgc gaggaactgg tggactacga gtggcaggag
     5941 tccgaagcgg ggcctccccg caagtactac aagctcactc agaagggccg cacgcaactg
     6001 tcgacgctca atgattactg gaaagatttg aaccgcacac tcgcggaatt tgggaattaa
     6061 gccatgcaaa aagtggtcag catcagcctc aacggcatcg cctatcagct cgaagagcca
     6121 ggctacaacc agctccgctc ctacctcgag cgcgcggaat cgcgcctcaa ggacagcccg
     6181 gatcgcaccg aggtcatgaa cgacctcgag caggcgatcg gcgagaaatg cgcgcgcgta
     6241 ctggggccgc acaagacggt cgtcaacgaa gcggaagtcg ccaggatcat cgaagagatg
     6301 gggccggtcg aatctgccga ggagaaggct gccggcgcgg gggcagcgcc cgaaggcgcg
     6361 cccgagcagg agtccgctta cgtgggtccc cagccgcgca agcgtctgtt caacatccgc
     6421 gaaggcgcga tgtgggccgg cgtgtgcaac ggcatcgccg catacatcaa cgtcgacgtc
     6481 acctgggtgc gcattgcgtt cgcgctcatc accatcttca gctggggcgg gatgatcctg
     6541 gtgtacatcg ccctggcgtt catcgtgccc acggcggaaa ccgccgagga ccgcgctgcg
     6601 gcgttcggta tgcccttcaa cactgaagag ctcataggtc gcgctaaaaa aaactttgaa
     6661 aagttcggct cggactaccg ctggcggcgc gagtggcgcc gccagcagcg ccactggaat
     6721 cgccagtggc atcagatgag cgagcaggtg cgccaggcca cggcccaggc ggcaccccag
     6781 atgggcacca ccgcgcgcgc catcatgagc atcttcctgc ccatcgcggc cattgtcgga
     6841 gccgtactgt tcgtcgtctg gatcctggcg atgctctcgc tggtcacgca gcacaccgta
     6901 ttcgggtggg ggctgccgca cggcatgccg ctgtgggtgg gcctcctcgt gctggtcctg
     6961 gtctacttcg cgatctccgc gccgctcaag gcgattcgac atggcggccg cgaggccgcg
     7021 ggcagtcatc cgggttgggg cgcattgcac ggcctgatgt ggatgggatt caccgtgctg
     7081 tttttctggc tcgcgtatac cttcttcccg ggagttcgtg agctcgtgga tcagctcatg
     7141 tgggcggcgg atctcacggt ctcgaacatt tcggagacca ttacttttgg gagctgggat
     7201 tgaagtcgct cgaaaccgtc accgtcgaaa caggacccaa ccccactttc tccatcatct
     7261 ggatgcacgg cctcggcgcc gacggccacg acttcgagcc gctggtgccc gaattgctgg
     7321 aagacggcat gccggtcatc cgcttcgtgt ttcctcacgc gccggtgcgt cccgtgacca
     7381 tcaacaacgg ttacgagatg cgggcgtggt acgacatcat cggcatcgat cggcgctcgg
     7441 ccgaggactt cgacggaata aacgcctccg cgcaggcgat cgcagcgctc gtccagcgcg
     7501 agaacgagcg cggcattccc acgaaccgca tcatcgtcgc cggtttctcc cagggcggcg
     7561 ccatggcgct gcacatcgcg acgcgctacc cgcagacctt cgccggcgtg atcgccctgt
     7621 cctgctatct acccgtggcg cgtgacttgc ctgcggctca taacgcggcc aatctcggca
     7681 cgcccatctt catggcgcac ggttcgcagg atcccgtggt accgatcgcg ctgggcgagt
     7741 tttcgcgcga agcgctcgag gccgtgggtt atggcgtgga atggcacacg tatctcatgc
     7801 cacattcgct ttgccctgac gaagtcgctc acttgaaatc ctggctggcc aggcgcgttg
     7861 cccaggtgtg agccacgcgc cgcggtttca cgacgtgcgg ttgagctcgt acaggcatat
     7921 gttcaccgtc gccgccacgt tcagcgattc gatggcgccc gttcccgcga tcgtgaacgg
     7981 ccgggcaccc agggcatcca tcttttcgcg cggcaggccg cgcgcctcgt tgccaaacac
     8041 ataacaatcg aattcccgaa acgcgggcga cggaaccggt tgccccccga gatcgagcga
     8101 ggcgatgcgc gcgaatcgtt ttggcaagga gtccatcgga acatcgaact ccacagcgac
     8161 gtgaaagatc gcacccatgc tcgcacgcac caccttgccg ttgtgcaggt ccacgctgtc
     8221 tggactgcag aggcaccgga agtttccgaa ccaggccagc gtgcgcagga tggtgccgaa
     8281 attcccgggg tcctggatct cgtgaagata gaccgcgcgt tcgccggcgc gtggcgcggc
     8341 gggctcgagc aacgggacca cggcggcgat gccttgcggt gaacgcgttt cactgatctg
     8401 cgccatgtgc ttggactgga tgacgtgcat cggcagcgca ctcgaccagt gcgcatagtc
     8461 gcgcgtcacg aaaatcttgc tctcccgcag gcgcgcatcg cgcgtcgcgg cgcgttcgag
     8521 ctcctgcacg agatgttcac cttcgatgac gaagtgcttg aacttctccc ggaatttctt
     8581 ctcgtgcagc ttcttgatgt cgtcgaattt catgcgagat cccgcgcgac cgcttcggcc
     8641 accttgatgc catcgacccc cgcggagagg attccgccgg cgtatccggc accttcaccg
     8701 caagggtaca ggccgcggac attgagactc tgaagtgtgt ctgcattgcg cgtgatgcgt
     8761 accggtgacg atgtccggct ttccacactc gtgagcaccg cgtcgtcgcg gtcgaagcca
     8821 gggatctgct tgccgaacat cggcagcgct tcgcggatgg cgtcgattgc gtaggccggc
     8881 agcgagggcg acagatcgca gagcttcacg cccggctggt acgacggctg gacctcgcct
     8941 agctgagtgg aaggcttgcc gcgcacgaaa tcaccgagga gctggccggg tgcgcagtag
     9001 tcgccgccgc cgagctcgaa tgccgtcgat tcgagctgct cctgcaaggc gactccggcg
     9061 agcggccctc ccggaaaatc aggttcagga ttgatagcga cgacgatgcc cgcattcgca
     9121 ttgcgttcgt tgcgcgagta ctggctcatg ccgttggtga cgacgcgccc ggcctcggag
     9181 gtcgccgcca cgaccatgcc gcccggacac atgcagaagc tgtacaccga gcgtccattg
     9241 ctggcgtgat gcaccacctt gtagtcagcg gccccgagct ccgggtgacc ggcgaaacga
     9301 ccgaaccgcg cgcggtcgat gagcgactgc gggtgttcga tgcgaaaacc aatcgcgaag
     9361 ggtttcgcct cgagaaagac gccgcgccgc tccagcatgc gcagcgtgtc gcgcgagctg
     9421 tgtccgagcg cgagcaccac gtaccggctg gacagcgtct cgccgttctc gagcacgaca
     9481 ccggcgatcc tcccgaggtc gatcaacacg tccgtcacct tgctttcgaa gcgcacctcg
     9541 ccgccgagcg cgatgatctc cgcgcgcatc ttctcgacga tgccggtgag ccggaaagtg
     9601 ccgatgtgcg gcttgctgac gaagagaatc tcttcgggcg cgcccgcgcg cacgaactcg
     9661 tgcatcacct tgcggccgta gaatttcgga tccttgatct ggctgtagag cttgccgtcg
     9721 gagaacaggc ccgccccacc ctcgccgaac tgcacgttgg attcgggcat cagtaccttc
     9781 ttgcgccaca gcccccaggt gtcctgcgtg cgccggcgta cgtcgcggcc acgctcgagc
     9841 acgatgggct tgaagcccat ctgcgccagc agcaacgccg cgaacaaacc gccggggccg
     9901 aagccgacta cg
//
LOCUS       DASQJS010000215         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1689709, whole genome shotgun sequence.
ACCESSION   DASQJS010000215 DASQJS010000000
VERSION     DASQJS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689709"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..783
                     /gene="wrbA"
                     /locus_tag="VEW08_08785"
     CDS             169..783
                     /gene="wrbA"
                     /locus_tag="VEW08_08785"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HYJ40872.1"
                     /translation="MTEILIVYYTRHGATAELARQVARGVESVAGCTATLRTVPAVSA
                     ESERPVKPVPDAGAPYATLDDLRRADGLLLGSPTRFGNMAAPLKYFLDGTSSLWLNGT
                     LSGKPAGVFSASQTMHGGQESTLLTMAMPLLHHGMLLVGIPFTERSLNQTRTGGTPYG
                     VTHVSGLATQPGVLSEDEIALAQAQGRRVAEVAAALGAGRAARK"
     gene            complement(793..1491)
                     /gene="hda"
                     /locus_tag="VEW08_08790"
     CDS             complement(793..1491)
                     /gene="hda"
                     /locus_tag="VEW08_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HYJ40873.1"
                     /translation="MRQLTLGVQLKERATFASFLTARNVELVAHLHHLAANTPPGATW
                     IAGPHTAGKSHLLQAVCAATAAGKRAGYLPLETLLPFGPAALEGVEQLEVACYDDVQV
                     IAGLEDWEHRLFSLWQRALERSATLLFTARESPAQISFGLPDLKSRLASSAVFAVREL
                     NDEEQVEALNLRADLRGFELPAETARYLQHRFPRDMRTLCEVLDTLDDAAFAAQRRIT
                     VPFIRDVIDGRVKR"
     gene            complement(1507..2046)
                     /locus_tag="VEW08_08795"
     CDS             complement(1507..2046)
                     /locus_tag="VEW08_08795"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYJ40874.1"
                     /translation="MLRHLPNFICLVRIALIWPTIDALYAGHFWTALILVAVCAISDG
                     LDGWLAKRFNWTSHLGKILDPLADKLLLVALFLTSAWMNLLPWWLTAIVVARDVMIGA
                     GAVIYRFWFGPLHGRPTLLSKINTGMQLAVALAAILGAAAGMPTPEMVTALAILTMLT
                     TIVSGADYLSAFTRRALAT"
     gene            complement(2058..>2876)
                     /locus_tag="VEW08_08800"
     CDS             complement(2058..>2876)
                     /locus_tag="VEW08_08800"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HYJ40875.1"
                     /translation="VVLVRATGARDAANDPALAQVLAQAQTYVLGTRPAGTGSAITVM
                     FDGAALERDILAAGRTVWPTDRPVVLVILTGGPATGAFETRRQVEGALDAAGNRRGQP
                     ITIARPEALNLPTTGDISPEAALAAAQRMRADGVLVGYGDAVANGGTWRWSLNAPGVS
                     EAWNGTLEEGVHGAADVFVRTAVSYAALPEISILVEIEGVPTLKEYARAAEILAEAPG
                     VRGVQLAEAAGSRTTFSVLTRGGGDALLTSLGGNPRLERMDPTAGGNLAFRLRQ"
BASE COUNT          524 a          957 c          950 g          445 t
ORIGIN      
        1 gacgacttcg acacggccat ccgagaggtt gcgggcaaag ccggtcaacc cgagagactc
       61 ggccttgcgc acgcaggtcg cccgatagaa gacgccctgc acccggcccg aaacaaagga
      121 acgcctgcag atcatgcggc atgatagtca tcaataagaa gagtggccat gaccgaaatt
      181 ctcattgtct actacacgcg acacggcgcc acggcggagc tcgcccgcca ggtggcgcgc
      241 ggcgtggaaa gtgtcgccgg ttgcaccgcc acgttgcgca ccgtgccagc cgtgagcgcc
      301 gagagtgaac ggccggtcaa acccgtgccc gacgctggag cgccttacgc cacgctcgac
      361 gatctgcgcc gcgccgatgg actgttgtta ggcagcccga cgcgcttcgg aaacatggcc
      421 gcgccgctca agtattttct cgatggcacc tcgagcctgt ggctgaacgg aacgttgtcg
      481 ggaaaacccg ccggcgtgtt ctccgcgtcg cagacgatgc acggaggaca ggaatcgacg
      541 ctgctgacga tggccatgcc gcttctgcat cacggaatgc tgctggtggg catccccttc
      601 accgagcgca gcctgaacca gacgcgcacc ggcggaacgc cgtatggcgt cacgcatgtg
      661 tccggcctcg ccacccagcc tggcgtgctg tccgaagatg aaatcgcgct cgcccaggcg
      721 cagggtcgcc gtgtcgcgga agttgccgcc gcgctcggcg cgggacgcgc ggcgcgaaaa
      781 taactacctg cgctaccgct ttaccctgcc gtcgatcacg tcgcgaatga acggcacggt
      841 gatgcgccgt tgcgcggcga acgctgcatc gtcgagcgta tcgagaacct cgcacaacgt
      901 acgcatgtcc cgcggaaagc ggtgctgcag ataacgcgcc gtttccgccg gcagctcgaa
      961 cccgcgcaga tccgcgcgca ggttcagcgc ctcgacctgt tcttcgtcgt tgagttcacg
     1021 caccgcgaag accgcggatg aagccagccg cgacttcagg tccggaagac cgaaggaaat
     1081 ctgcgccggg ctctcgcgcg ccgtgaacaa caacgtcgca ctgcgctcca gcgcgcgctg
     1141 ccacaacgaa aacaagcggt gctcccagtc ttcgagtccc gcgatgacct gcacgtcgtc
     1201 ataacaggcg acttcgagct gttcgacccc ttcgagcgcc gccgggccga acggcagcag
     1261 ggtttcgagc ggcagatagc cggcacgttt tccggcggcg gtggccgcgc ataccgcctg
     1321 cagcagatgc gacttgccgg cggtgtgcgg ccccgcgatc caggtcgccc cgggtggagt
     1381 attcgccgcg aggtgatgca ggtgcgcgac cagctcgaca ttgcgcgcgg tgaggaagct
     1441 cgcaaaggtg gcgcgctcct tgagttgcac gccgagcgtc agctgtcgca tcggaaggcc
     1501 tttgggtcag gtcgcgagcg cgcgccgcgt gaaggcggat agatagtctg cgcccgagac
     1561 gatggtggtg agcatcgtca ggatggccag cgcggtcacc atctcaggcg tcggcatgcc
     1621 tgcggccgca ccgagaatcg ccgccagtgc gacggcgagc tgcatgccgg tgttgatctt
     1681 ggagagcaag gtgggccggc cgtgcaacgg cccgaaccag aagcggtaga tgaccgcgcc
     1741 tgcgcctatc atcacatcgc gcgccacgac tattgccgtc agccaccaag gcagcaggtt
     1801 catccacgcc gaggtcagga acagcgcgac cagcagtagt ttgtcggcga ggggatcgag
     1861 aatcttgccg agatgcgaag tccagttgaa tcgcttggcc agccagccgt cgagcccgtc
     1921 ggagatcgcg cacacggcca cgagaatcaa cgcagtccag aaatggcccg cgtacagcgc
     1981 atcgatggtc ggccagatga gcgcgatgcg caccaggcat atgaaatttg gaaggtgacg
     2041 caacatcgcg cgctcgacta ctgacgcagg cgaaatgcta ggttaccgcc ggcggtggga
     2101 tccattcgtt ccagtcgcgg gttgccgccg agtgaggtca gcaaggcatc cccgccgccg
     2161 cgcgtcaaca cagagaaagt tgtgcgcgaa cccgccgctt cggcaagttg cacgccgcgc
     2221 acaccgggcg cttccgccag gatctccgcg gcgcgcgcat attccttgag cgtgggcacg
     2281 ccttcgattt cgacgaggat ggaaatttcg ggcagcgccg cgtaggaaac cgcagtgcgc
     2341 acgaacacat cggcggcgcc atgcacaccc tcttccagcg tgccattcca ggcttcactg
     2401 actcccggcg cgttcagcga ccagcgccag gtcccgccat tggcgacggc gtcgccgtaa
     2461 cccaccagca caccgtcggc gcgcatgcgt tgtgcggccg cgagcgccgc ttccggtgaa
     2521 atgtcgccgg tagttggaag attcagcgcc tccgggcgcg cgatggtgat gggctgtccg
     2581 cggcggttgc cggcggcatc gagcgcgcct tcgacctggc ggcgtgtctc gaaggcgccc
     2641 gtggcgggcc cgcccgtgag gatgaccagc accaccggac gatcggtggg ccacaccgtg
     2701 cggccggccg cgaggatgtc gcgctcgagc gcggccccgt cgaacatcac tgtgatcgcg
     2761 ctgccggtgc ccgcgggacg tgtgccgagc acgtaggtct gcgcctgtgc gagcacctgg
     2821 gccagcgccg ggtcattggc cgcgtcgcgc gcacccgtgg ctcgcaccag caccac
//
LOCUS       DASQJS010000216         7594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3575366, whole genome shotgun sequence.
ACCESSION   DASQJS010000216 DASQJS010000000
VERSION     DASQJS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3575366"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VEW08_08805"
     CDS             <1..423
                     /locus_tag="VEW08_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYJ40876.1"
                     /translation="FGFREFARLKQGGIPPEQLLEGARRAMRVGQLKELERLEESLAS
                     LATIGSTSPYVGLFGTVWGILNSFVALGGVQQATLQMVAPGIAEALIATAMGLFAAIP
                     AVIAYNRYADQVSRLELRYDAFMEEFSTILQRYAGRAG"
     gene            436..888
                     /gene="tolR"
                     /locus_tag="VEW08_08810"
     CDS             436..888
                     /gene="tolR"
                     /locus_tag="VEW08_08810"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HYJ40877.1"
                     /translation="MASSQPRRRRKLMGEINVVPYIDVMLVLLIIFMITAPLLAEGIK
                     VELPKAGARPIPPEMLKDSKPIVLTIDEAGRLFLNYGAKQDEPLDAAAVEAAAGAVLR
                     RAPETAVLVRGDYRVAYGEVVGAMTILQRAGASKVGFITQPPEQKRAR"
     gene            943..1806
                     /gene="tolA"
                     /locus_tag="VEW08_08815"
     CDS             943..1806
                     /gene="tolA"
                     /locus_tag="VEW08_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HYJ40878.1"
                     /translation="MALSILLHSAIVAAAVYGWFTWKHRPPPVETLAIEATVVDERAL
                     KGIATKPEPAPPPPEPEPVPEPVPEPPPVEEQGPPKPDPAELQRKEEERLAQAKLNEE
                     RAEKEKAELKAAQDLKEREDAEKLEAEKKAAAKAAADKAAADKAAADKKALDKKRADD
                     AAAAKKAAEEKARQARQAELQRALEAEERGNAMRNSDEANRWHAQIIARVTRAWIKPP
                     SAQPGISCIVSVTQVPGGEVTAVNVNSCSINDAALRESVEAAVLRASPLPPPPDPALF
                     ERNLELTFAPQ"
     gene            1815..3125
                     /gene="tolB"
                     /locus_tag="VEW08_08820"
     CDS             1815..3125
                     /gene="tolB"
                     /locus_tag="VEW08_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HYJ40879.1"
                     /translation="MKKLATVLSIALLLTGIGAATPSWAQLRLTITQGVNDPIPIAIV
                     PFSRAVPADGGLDVAQVIQRDLESTGRFKAMAREDMVFKPTASADIDAAGWKQQRVDY
                     VAVGRVTSLADGQVRIDAELVNVLTGQRVAGPNVTTAASNLRNAAHRIADALFEKIIG
                     IRGAFATRIAYVSVDGKPPKQTYELYVADADGANRVKILASPLPIMSPAWSTDGEWLA
                     YVSFERRVSAVYVQHRRTGKKSMVSARAGINGAPTFSPDDKKLALTLSGSNGNLDVYL
                     LELASGKLTRLTDDPAIDTEAVFTPDGTGIYFTSDRSGNPQIYRMTLGSSERPKRVTF
                     TGSYNARPRLSPDGKQLALVTLDNGAYRIGVQDLANGTVTMLSKGRQEESPSFAPNGA
                     MLIFGGRERGQGVLQTVSVDGQVSARLDADAGEVREPVWGPFLP"
     gene            3164..3679
                     /gene="pal"
                     /locus_tag="VEW08_08825"
     CDS             3164..3679
                     /gene="pal"
                     /locus_tag="VEW08_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HYJ40880.1"
                     /translation="MKNFASVSVLLATLALLAGCPSKKADVPPPAPAPTTDTSGVEDP
                     NATMGDTDASMGPSGELLSKRIVYFDLDRADIRADSASVVAAHAAYLAKNPNQKVRLE
                     GHADERGSREYNIGLGERRGQSVRRALALQGVAETQLSTVSYGEERPAAAGSDEMAYA
                     ANRRVEIVYLK"
     gene            3711..4451
                     /gene="ybgF"
                     /locus_tag="VEW08_08830"
     CDS             3711..4451
                     /gene="ybgF"
                     /locus_tag="VEW08_08830"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HYJ40881.1"
                     /translation="MNTSRLLVVAAAGLALGGCATPPEEDPVFIRMNDLDARVQRIER
                     VLTNQSLLELAQRLDALQADVRTIRGEVELLQNETQGGKTQSRNLYGDLEKRLAALET
                     LGGVGGAPAIGATSAPAAGASEQATYDAGMAALRSGNYDKAVATFREVVTNYPTGDLA
                     PNAQFWIGESYYTKGDLESAVTSYRKVLGDWPNSRKAPDAMVKLGFSLSDLKRTGEAR
                     STLEEVVRKYPGTPAAQLAADRLKRLGR"
     gene            4477..5154
                     /gene="queE"
                     /locus_tag="VEW08_08835"
     CDS             4477..5154
                     /gene="queE"
                     /locus_tag="VEW08_08835"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016711990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HYJ40882.1"
                     /translation="MNAAASELEPSGERRLKITEIFYSLQGEADTVGFPTVFVRLTGC
                     PLRCHFCDTAYAFHGGEWRSVEEVLATVGGYAPRYVCVTGGEPLAQKNCLPLLTRLCD
                     ANYRVSIETSGAMPLGGVDARVIRVVDVKTPGSGEERRNRYDDLSLLRPEEQIKFVIC
                     DRADYEWSRDKLSSLKLGDRCQVLFSPSAEQLPARELADWILADRLPVRFQMQLHKVL
                     WGNVPGK"
     gene            5156..5860
                     /gene="queC"
                     /locus_tag="VEW08_08840"
     CDS             5156..5860
                     /gene="queC"
                     /locus_tag="VEW08_08840"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005399706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HYJ40883.1"
                     /translation="MSPRAVVLLSGGLDSATVLALARASGLECYALSVSYGQRHQAEI
                     SAAARVAHALGAREHRIMHVNLGRIGGSALTDSSIDVPTSPGEGIPITYVPARNTIML
                     SLALAWAEVLKATEIHIGVNAVDYSGYPDCRPEFVAAFQELAALATKAGVEGGNLRVL
                     APLIHLSKAQIVREGVRLGVDYSLTVSCYQADDEGRACGLCDSCRLRREGFVSAGVPD
                     PTRYLRFAGQVDEKIV"
     gene            5879..5954
                     /locus_tag="VEW08_08845"
     tRNA            5879..5954
                     /locus_tag="VEW08_08845"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5912..5914,aa:Lys,seq:ttt)
     gene            5977..>7594
                     /locus_tag="VEW08_08850"
     CDS             5977..>7594
                     /locus_tag="VEW08_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HYJ40884.1"
                     /translation="MSDPEFDYVIIGAGTAGCLLANRLSADPSCRVLLVEAGPPDNYA
                     WIHIPVGYLRCIGNPRTDWRYRTEPEAALHGRDLLYPRGKTLGGSSSINGMIYMRGQA
                     RDYDAWASATGDDAWRWSNVLPDFRAHECSFRCVDDEHPHHGARGEWRVSTQRLRWDI
                     LDAFAEAAEQAGFARIPDFNTGDNAGVSYFDVNQRGGFRVSSAKAFLKPARARSNLTV
                     WTHAMTERLQFERGANGVPRCLGAALIHNGQRVHVRATRDVILSAGAIGTPQLLQLSG
                     IGPASLLREHGIEVLLDSPGVGANLQDHLQIRPVFKVRGVRTLNALASSFWGRLAIGL
                     EYALRRTGPMSMAPSQLGAFVRSDPARVHANLQYHVQPLSLRAYDQPPDTFPAFTASV
                     CNLNPESRGTVRIRSASVADPPRIAPNYLSTDEDRRIAAESLRVTRRIVTQPALARYF
                     PEEYLPGAQFQTDEELVREGGRIANTIFHPVGTARMGDASDPAAVVDSHLRVRGVDGL
                     RVVDASVMPTITSGNTNSPTLMIAEKAARWI"
BASE COUNT         1391 a         2472 c         2462 g         1269 t
ORIGIN      
        1 ttcggctttc gcgagttcgc gcgcctgaag cagggcggca taccgccgga gcagttgtta
       61 gagggggcac gccgggccat gcgcgtgggc cagctcaagg agctggagcg tctcgaggag
      121 agcctcgcga gcctcgccac catcggttcc accagtccgt acgtgggcct cttcggcacg
      181 gtgtggggca tcctcaattc attcgtcgcg ctgggcggcg tgcagcaggc aacgctgcag
      241 atggtcgcac ccggaattgc ggaggcgctg atcgccaccg ccatgggatt gtttgccgcc
      301 atacccgcgg tgatcgccta caaccgctat gccgaccagg tgagccggct cgaactccgc
      361 tacgacgcgt tcatggaaga gttctccacc attctgcagc gctacgccgg ccgcgccggc
      421 tagaggagat aggccatggc atcctcacaa ccgcgccgcc ggcgcaaact catgggcgaa
      481 atcaacgtcg tgccctacat cgatgtgatg ctggtgctgc tcatcatctt catgatcacg
      541 gcgcccttgc tggccgaagg catcaaggtg gagctgccca aagccggtgc acggccgatt
      601 ccgcccgaga tgctcaagga ttcaaagccc atcgtgctca ccatcgacga ggcggggcgc
      661 ctgttcctca actacggggc gaagcaggat gaacccctcg atgcggccgc ggtcgaagct
      721 gcagcgggtg cggttcttcg ccgtgcgccc gaaactgccg tgctggtgcg cggtgactat
      781 cgcgtggcgt atggagaagt cgtcggagcc atgaccatcc tgcagcgtgc cggcgcgagc
      841 aaagtcgggt tcatcacgca gcctccggaa cagaagcggg cccgctgacc cacgatgccg
      901 ccgcgctccg gtaagtcatt gacgaagggc ggatggcgcg caatggcgct gtccatcctg
      961 ctgcactccg cgatcgtcgc ggccgcggtt tatggctggt tcacgtggaa acatcgtccg
     1021 ccgccggtcg aaacgctcgc catcgaagcc acggtggtcg acgagcgcgc gctgaaaggc
     1081 atcgcgacga agcccgaacc cgcaccgccg ccccccgagc cggaaccggt tcccgaaccc
     1141 gtgcccgaac cccctccggt cgaagaacag gggccgccca aacccgatcc ggccgagctc
     1201 cagcgcaagg aagaagagcg cctcgcccag gccaagttaa atgaagaaag ggccgagaag
     1261 gaaaaggcgg agctgaaggc tgcgcaggat ctcaaggagc gcgaggacgc cgagaagctc
     1321 gaagccgaga agaaggcggc ggcgaaggcg gccgctgaca aggctgcggc tgacaaagcc
     1381 gctgccgaca agaaggcgct cgataaaaag cgcgccgacg atgccgctgc cgcgaagaag
     1441 gccgccgagg agaaggcgcg gcaggcgcgc caggcggaac ttcagcgcgc gctcgaggcc
     1501 gaagaacggg gcaatgccat gcgcaacagc gatgaagcaa atcgatggca tgcgcagatc
     1561 atcgcgcgcg tgacgagggc ctggatcaaa ccaccctcgg cacagcccgg catctcttgt
     1621 attgtgtcgg tcacccaggt tcccggcggc gaggtgactg ccgtcaacgt gaacagctgc
     1681 agcatcaacg atgccgcgct gcgtgaatcg gtcgaagccg cggttttacg ggcatcgccg
     1741 ttgcctccgc ctccggatcc ggcgctgttc gagcgcaatt tggagctaac attcgcccct
     1801 caatgaacga gcgcatgaaa aaactagcca ctgttctatc gattgccctg ctgctgactg
     1861 gcatcggcgc ggccacgccc tcgtgggcgc agcttcgcct caccatcacg cagggcgtca
     1921 acgatcccat tccgattgcc atcgtgccgt tctcacgcgc ggtgccggcg gacggcgggc
     1981 tcgacgtggc gcaggtcatc cagcgcgacc tcgagagcac cggccggttc aaggccatgg
     2041 cgcgcgaaga catggtgttc aagccgaccg cttcggccga catcgatgca gccggctgga
     2101 aacagcagcg cgtggactac gtcgccgtgg gccgtgtgac gtcgctcgcc gacggacagg
     2161 tacgcatcga cgccgaactc gtcaacgtgc tgaccggcca gcgcgtggcc ggtcccaacg
     2221 tcaccacggc ggcctcgaac ctgcgcaacg ccgcgcatcg catcgcggat gcgttgttcg
     2281 aaaagatcat cggcatacgc ggtgcattcg ccacgcgtat cgcctacgtt tcggtcgacg
     2341 gcaagccgcc gaagcagacc tacgagctgt acgtggccga tgccgatggc gcgaatcgag
     2401 tgaaaatcct ggcatcgcca ctgcccatca tgtcgccggc ctggtcgacg gatggagagt
     2461 ggctggccta tgtgtcgttc gagaggcgcg tgtccgccgt ctacgttcag caccggcgca
     2521 ccggcaagaa atccatggta tcggcccgcg ccggcatcaa cggcgcgccc acgttttctc
     2581 ccgatgacaa gaaactggcg ctcacgctgt cgggcagcaa tggcaacctc gacgtctatc
     2641 ttctcgagct cgccagtgga aagctcacgc gcctgaccga cgatcccgcc atcgacacgg
     2701 aagcggtctt cacacccgac ggtaccggca tctacttcac ctcggatcgc tccggcaatc
     2761 cgcagatcta tcgcatgacg ctcggaagtt cggagcggcc gaagcgcgtc accttcacgg
     2821 gcagctacaa tgcgcgcccg cgtctctcgc cggacggcaa gcaactcgcg ctggtcacgc
     2881 tggacaatgg tgcgtatcgc atcggcgtgc aggacctggc gaacggcacg gtgaccatgc
     2941 tttcgaaggg gcgccaggaa gaatctccga gcttcgcgcc gaacggcgcc atgttgatct
     3001 tcggcggccg cgaacgcggc cagggtgtgc tgcagacggt gtccgtcgac ggccaggtct
     3061 cggcgcggct cgacgccgac gcgggtgaag ttcgtgaacc ggtgtgggga cctttcctgc
     3121 catgaaacat tcagtaaatt cagttatgac gacaggtgtg caaatgaaaa atttcgcttc
     3181 cgtttcggtt ctcctcgcaa cgcttgcact gctcgcgggc tgccccagca aaaaagctga
     3241 cgtgccgccg ccggcgcctg cgccgaccac cgacaccagc ggtgtcgaag atccgaacgc
     3301 cacgatgggc gacaccgacg cgtcgatggg tccgtcgggt gaactgctct ccaagcgcat
     3361 cgtgtatttc gacctcgacc gcgccgacat tcgcgccgat tccgccagcg tggttgccgc
     3421 gcatgcggct tatctcgcga agaacccgaa tcagaaagtg cgcctcgaag ggcacgctga
     3481 cgaacgcggc tcgcgcgagt acaacattgg cctgggtgaa cgccgcggcc agtcggtgcg
     3541 ccgcgcgctg gcgctgcagg gcgtggccga aacgcagctg tccaccgtga gctacggcga
     3601 agagcgcccg gcggccgcgg gcagcgacga aatggcgtac gcggccaatc gccgcgtcga
     3661 aatagtctat ttgaagtagt cgccagatga aggtctggag tggggtgccc gtgaacacat
     3721 cgcgtttatt ggtcgtggcc gctgccgggc tggcgctcgg tggctgcgcg acgccgcccg
     3781 aagaggatcc ggtgttcatc cgcatgaacg acctcgatgc gcgcgtgcag cgcatcgaac
     3841 gcgtgttgac caaccagagc ctgctcgaac tggcgcagcg cctcgatgcc ttgcaggcgg
     3901 acgttcgcac catccgcggc gaagtcgagt tgctgcagaa cgaaacgcag ggcggcaaga
     3961 ctcaaagtcg caatctgtac ggggacctcg agaagcgcct ggcggcgctc gaaacgctgg
     4021 gtggcgtggg cggggcgcca gcgatcggcg caacttcggc cccggcggcc ggcgcatccg
     4081 aacaggccac ctacgacgcc ggcatggcgg cgctgcgctc cggcaattac gacaaggcag
     4141 tagccacgtt ccgcgaagtc gtcactaact acccgaccgg tgacctggcg cccaacgcgc
     4201 agttctggat cggcgagagc tactacacca agggcgatct cgaaagcgcc gtcacctcgt
     4261 accgtaaagt gctcggcgac tggccgaact cgcgcaaggc gcccgacgcg atggtcaaac
     4321 tcggcttctc gctctctgac ctgaagcgca ccggcgaggc gcgcagcacg ctcgaggaag
     4381 tggtgcgcaa atatccgggg acacccgccg ctcagctcgc tgcggatcgc ctgaagcgcc
     4441 tcggcagatg aacccccggt cgataaaaaa tattcgatga acgccgctgc aagcgagctc
     4501 gagcccagtg gcgaacgccg cctgaagatc acggaaattt tctactcgct gcagggcgag
     4561 gccgacaccg tgggttttcc gacggtgttc gtgcggctca ccgggtgccc tttgcgctgt
     4621 cacttttgcg acaccgccta cgcgtttcat ggcggtgagt ggcgcagcgt cgaggaagtg
     4681 ctggccacgg tcggtggata cgcaccgcgt tacgtgtgtg tcaccggcgg tgaaccgctg
     4741 gcacagaaga actgcctgcc gctgctaacc cgtctatgtg atgccaatta ccgcgtctcc
     4801 atcgagacca gcggcgccat gccgctcggc ggtgtggacg cgcgcgtgat ccgcgtcgtg
     4861 gacgtgaaga ctccggggtc gggcgaagag cggcgcaacc gttacgacga cctctcgctg
     4921 ctgcggcccg aagagcagat caagttcgtg atctgcgatc gcgccgacta cgagtggagt
     4981 cgcgacaaac tctcgagcct gaaactgggc gatcgctgcc aggtcctgtt ctcgcccagc
     5041 gccgaacagt tgcctgcgcg cgaactcgcc gactggattc tcgcggaccg gttgccggtt
     5101 cgtttccaga tgcagcttca caaggtgctc tggggcaacg tgccaggtaa atgacatgag
     5161 cccgcgagcg gtggtgttgc tgtcgggcgg gctggattca gccaccgtgc tcgcgcttgc
     5221 ccgtgcgtcg ggcctcgagt gttatgcgct gtcggtgtcg tatggacagc ggcaccaggc
     5281 ggagatcagc gctgccgcgc gcgtcgcgca cgctctcgga gcgcgcgagc atcgcatcat
     5341 gcacgtgaac ctcgggcgca tcggcggttc ggcgctgact gacagctcca tcgacgtgcc
     5401 cacgtcaccg ggcgagggca tcccgatcac ctacgttccc gcgcgcaaca ccatcatgtt
     5461 gtcgctggca cttgcctggg cggaggttct gaaagcgacc gagatccata tcggcgtaaa
     5521 cgcggtggac tattccggat atccggactg ccgcccggaa ttcgtcgcgg cattccagga
     5581 gctcgccgcg ctcgccacga aggcgggcgt ggagggtgga aacctgcgcg tgctggcgcc
     5641 cctcatccat ttgagcaagg cgcaaatcgt gcgcgaaggc gtgcgtttgg gcgtggacta
     5701 ttcattgacg gtctcttgct accaggcgga cgacgagggg cgcgcatgcg gcctctgtga
     5761 ttcctgtagg ttaaggcgcg aaggctttgt ttccgctgga gttcctgacc ccacgcgcta
     5821 cctccgtttc gcaggacaag tcgacgaaaa gatcgtgtag acttcgcgcc cttccggggg
     5881 gtcggtagct cagttggtag agcagcgccc ttttaaggcg ttagtcgtgg gttcgagtcc
     5941 cacccgaccc accaataaca acccccgggt ccgcaattga gcgatcccga attcgactac
     6001 gtgatcatcg gcgcgggcac tgcgggttgc ctgcttgcca atcgtctgag cgcggatccc
     6061 tcttgccgcg tgctgctcgt cgaagcaggc cctcctgaca actacgcgtg gatccacatt
     6121 cccgtgggct atctacgttg catcggcaac ccgcgcaccg actggcgtta tcgcaccgaa
     6181 ccggaagccg cgctgcacgg ccgcgacctg ctgtacccgc gcggcaagac gctgggtggc
     6241 agctcgagca tcaacggcat gatctacatg cgcggccagg cgcgcgacta cgatgcgtgg
     6301 gccagcgcca cgggtgatga cgcgtggcga tggtcgaacg tgctgccgga cttccgcgcc
     6361 cacgagtgca gttttcgatg tgtggacgat gagcacccgc atcacggtgc gcgcggcgag
     6421 tggcgggtgt ccacacaacg cctgcgctgg gacatcctcg atgcgtttgc cgaagccgcc
     6481 gaacaggccg gctttgcgcg gatcccggac ttcaacaccg gcgacaacgc cggcgtcagc
     6541 tacttcgacg tgaatcagcg cggcggcttt cgggtgagtt cggcgaaggc gttcctgaaa
     6601 ccggcgaggg cgcgatccaa tctcactgtc tggacccacg cgatgaccga gcggctgcag
     6661 ttcgagcgcg gcgcgaacgg cgtacccaga tgtttgggag cggcgctgat ccacaacggc
     6721 cagcgcgtgc acgtgcgcgc gacacgcgac gtgatccttt ccgccggtgc catcggtaca
     6781 ccgcagctcc tgcagttgtc gggcatcggc ccggcgtcgc tgttgcgcga acacggcatc
     6841 gaggtgctgc tcgattcacc tggtgtcggt gcaaatctgc aggatcacct gcagatccgg
     6901 ccggtgttca aggtgcgcgg cgtgcgcacg ctgaatgcgc tggcttcgag tttctgggga
     6961 cggcttgcca tcgggctcga gtatgcgttg cgccgcacgg gtccgatgag catggcgccc
     7021 tcacaactcg gcgcgttcgt gcgcagtgat cccgcccgcg ttcacgccaa tctgcagtac
     7081 cacgtgcagc cgctgtcgct gcgcgcctac gaccagccgc cggatacctt tccggcgttc
     7141 acggccagcg tctgcaatct caaccccgaa agccgtggca ccgtgcgcat tcgcagcgcc
     7201 agcgtggccg acccaccgcg catcgcgccc aactacctct cgacggacga ggaccggcgc
     7261 attgcggccg agtcgctgcg cgtcacacgg agaatcgtca cgcaaccggc gctggcccgg
     7321 tatttccccg aggagtacct gccgggcgcc cagttccaga ccgatgaaga actggtgcgc
     7381 gaaggtgggc gcatcgccaa caccatcttc cacccggtgg gcacggcgcg catgggggac
     7441 gcgtccgacc cggcggcggt ggtcgatagc catctacgag tccgtggcgt cgacggtctt
     7501 cgggtggtgg acgccagcgt catgcccacc atcaccagcg gcaacaccaa tagtcccacc
     7561 ttgatgatcg ccgagaaggc cgcgcgctgg atca
//
LOCUS       DASQJS010000217         4455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4521142, whole genome shotgun sequence.
ACCESSION   DASQJS010000217 DASQJS010000000
VERSION     DASQJS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4521142"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VEW08_08855"
     CDS             complement(<1..195)
                     /locus_tag="VEW08_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40885.1"
                     /translation="MRGIGTLKVLDERDNLSPSATPAGLLAISAHDLARWLQIQLAKG
                     ALPEGGRLFSEASSKEMWTPQ"
     gene            230..1552
                     /locus_tag="VEW08_08860"
     CDS             230..1552
                     /locus_tag="VEW08_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYJ40886.1"
                     /translation="MGVANRCISSLVEAGKLKWDDRVADHIPGFQMYDPWVTREMTVR
                     DLLVHRSGLGLGAGDLLVIPRGTLSRAEVVKRLRFIKPATSFRSGYAYDNILYIVAGH
                     VIDLVSGQPYEDYIREHLFKPAGMLHSTSDEAHRFATPNRASPHARIGGALRGIGPLR
                     LLDERDNLAPSSTPAGLLAISANDLARWLQIQLARGALPGGGRLFSEESSAEMWAPQT
                     IEPIGPALPGLEAMTPNFQQYALGWEVRDYGGAKIIWHSGGVFGFVTVVVLIPEKNVG
                     FAITQNSEDGQARFGLMYELLDHYLGRPAKDWPALITKVRKERFDGALAALNTKAAAP
                     VRSKPTLALAQYAGRYVDAWYGAIAIGSDPKGLTINFTNTPNMGGRLEHWQYDTFIAR
                     FDDPGIEAAYVTFGLGAEGKVERITMKAVSPLADFSFDYQDLLFTPGP"
     gene            1599..2831
                     /locus_tag="VEW08_08865"
     CDS             1599..2831
                     /locus_tag="VEW08_08865"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HYJ40887.1"
                     /translation="MAIVALVVAASGLAASPVGAQDVRARVARVLKATPLIDGHNDWP
                     EVLREREGDGRWTLDLRSRLGTSVELYNTDITRLRQGMVGGQFWSVWVSPNLPGPEQV
                     KETIEQIALVQSFAERYPEVFAMARTAADVRRIHKGGRIASLIGVEGGGQIDGSLGVL
                     RAYRAQGASYLTLTHSKNIEWADSATDDPKHDGLTEFGESVVRELNRLGMLVDLSHVS
                     EPTMLDALRVSKAPVMFSHSNARSLVDHPRNVSDAVLKQVAANNGVVMVNFVPIYISD
                     AFRLWSSDRDAEKTRLNAPPFGGLYIGQPERAAAALAAWEREHPQPSVTLAMVADHIE
                     HIVKIAGVEHVGIGSDFDGAGKTLPAGLSDVSTYPALLAELMRRGWSDGDIARLAGGN
                     ILRVLDEAESVERSMNRR"
     gene            2850..3164
                     /locus_tag="VEW08_08870"
     CDS             2850..3164
                     /locus_tag="VEW08_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40888.1"
                     /translation="MTADVYANDDGLRHGTRSPRHQGAATFNIVADADADLLGRIGAI
                     LNLLNVAPRRFHMEAREEGTATVNALVDCAEFQADLVARKLQRLTSIRDVVVEYTPVT
                     QG"
     gene            3177..3437
                     /locus_tag="VEW08_08875"
     CDS             3177..3437
                     /locus_tag="VEW08_08875"
                     /inference="COORDINATES: protein motif:HMM:NF028001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5062 family protein"
                     /protein_id="HYJ40889.1"
                     /translation="MKKLKHEGELFKAGLLAAMKYAQERGAVEFEPTDSASQKLLYVY
                     RLLVHDKMIQPLPEDQVAENTMRHKLAIWYSKQLPADHPLLK"
     gene            3434..>4455
                     /locus_tag="VEW08_08880"
     CDS             3434..>4455
                     /locus_tag="VEW08_08880"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ40890.1"
                     /translation="MTDRGANPIIHWFEDGKAFEAQWRSHSDAPPAARVVVADGKMTA
                     DEAYGLACQGTALLWRGDFQEARQMLSALGNRADRPQRRSRNSAATSVANSLASPADA
                     FHLYRQARSQRARTLNALLIPLNDDYSIPLRRAPDIRQACAEAYGPAESASVTSLRAL
                     LGVIGAHEWRTKGIDIPEAGGRIHPHFGVFAPIRREYVDLVSTTPLPAACVSSNVAFD
                     IGTGTGVLAAVLARRGIECVIATDLDPRALACARENVRQLGLTDRIDVVQADLFPEGR
                     AALVICNPPWVPARPSSPMENGIYDPESQMLRGFLKKLPGHLEPGGEGWLILSDLAEH
                     LGLRTRA"
BASE COUNT          835 a         1339 c         1452 g          829 t
ORIGIN      
        1 ctgcggcgtc cacatctcct tgctggaggc ttcactgaac agccgcccac cctcaggcaa
       61 tgctcccttc gcgagctgga tctgcaacca tcgcgccagg tcgtgcgcgc tgatcgcgag
      121 caatcctgca ggcgtggccg agggcgacag attgtcgcgc tcgtcgagga ccttgagagt
      181 tccgatgcca cgcacggcgc cgccaattcg tgcgtgcggc catgctctgt tgggcgttgc
      241 gaaccggtgc atctcgtcgc tggtcgaggc cggcaaactc aaatgggatg atcgcgtcgc
      301 ggatcacatc cccggctttc agatgtatga cccgtgggtg acacgcgaga tgacggtgcg
      361 cgacctgctc gtgcatcgca gcggactcgg tctcggtgca ggtgacttgc tggtcattcc
      421 acgcggcaca ctgtcgcgcg ccgaggtcgt caagcgcttg cgcttcatca agccggccac
      481 gagcttccgc agcggctacg cgtacgacaa cattctttat attgttgccg ggcacgtgat
      541 cgatctggtg agcgggcagc cttacgagga ctacattcgc gagcacctgt tcaagccggc
      601 tggaatgctc cactccacca gcgatgaggc gcatcggttc gccacgccga atcgtgcatc
      661 tccgcacgcg cgcattggag gtgcgctgcg cggaatcggg ccgctgcggc tgctcgatga
      721 gcgggacaac ctggccccat cctcaacgcc tgcagggctg ctcgccatca gcgccaacga
      781 tctcgcgcga tggctgcaga ttcaattggc cagaggggca ttgcccgggg gcgggcggct
      841 cttcagcgag gaatcgagcg ccgagatgtg ggccccgcag acgatcgagc ccatcgggcc
      901 cgcgctgccg ggccttgaag caatgacgcc gaactttcag caatatgcgc tgggatggga
      961 ggtgcgcgac tacggtggcg cgaagatcat ctggcattcg ggcggcgtct tcgggttcgt
     1021 caccgtcgtc gtgctgatcc cggagaagaa cgtcggcttc gcgatcaccc agaacagcga
     1081 ggatggtcag gcgcggttcg ggctcatgta cgagctgctc gaccactatc tcggtcgccc
     1141 ggcgaaggac tggccagctc tgatcaccaa ggtgcgcaag gaacgcttcg atggcgcgct
     1201 tgcggcactc aataccaagg cggcggcacc cgtgcgctcg aaaccgacgc tggcgctggc
     1261 ccaatatgcc ggtcgctatg tggatgcgtg gtacggcgcc atcgccattg ggtccgatcc
     1321 caagggtctc acgatcaatt tcaccaacac gccgaacatg ggcggacgac tggagcactg
     1381 gcagtacgac acgttcatcg cgcgtttcga cgacccgggg atcgaagccg cctatgtgac
     1441 tttcggtctt ggcgccgaag gcaaagtcga gagaattacg atgaaggcgg tgtcaccgct
     1501 tgctgatttc agttttgact atcaggatct gcttttcaca ccaggtccct gacatgcgaa
     1561 gagtagctgg gggcgccagg cctcgccgcg gcgtttcaat ggcaattgtc gcgctggtag
     1621 ttgcggcatc gggcctcgcg gcatcgcccg tgggcgcgca ggacgttcgc gcgcgagtgg
     1681 cccgcgtgtt gaaggcgact ccgttgatcg acggacacaa cgattggccc gaggtgctgc
     1741 gcgaacgtga aggtgatggg cgctggacgc tggacctgcg cagcaggctg ggaaccagtg
     1801 tcgagctcta caacacggac atcacccggc ttcggcaggg catggtcggg gggcaattct
     1861 ggtccgtatg ggtgtcgccc aatctgccgg ggcctgaaca ggtcaaagag acgatcgagc
     1921 agatcgcgct cgttcagtca ttcgcggagc gctatcccga ggtgtttgcg atggcccgca
     1981 ctgctgcgga tgttcgccgc atccacaagg gcggacgaat tgcgtcgctg atcggtgtag
     2041 aaggcggcgg acagatcgat ggcagcctcg gcgtgttgcg cgcctatcgc gcccagggcg
     2101 cgagttacct cacactgacg cattccaaga acatcgagtg ggcggattcc gcgacggacg
     2161 atcccaagca cgacgggctg accgagttcg gcgagtcggt cgtgcgcgaa ctgaatcgcc
     2221 tcggcatgct ggtcgatctg agccacgtga gcgagccgac gatgctcgat gcactgcgcg
     2281 tgtcgaaagc accagtgatg ttttcgcact cgaatgcgcg ctcgctcgtt gaccatccac
     2341 gcaatgtctc cgatgcggtg ctgaagcagg tcgccgccaa caatggcgtg gtgatggtga
     2401 acttcgtgcc aatctacatt tccgatgcat tcagactctg gtcatcggac cgcgacgcgg
     2461 agaagacgcg gctcaacgca ccgccattcg gtgggcttta tatcgggcag ccggagcgcg
     2521 cggcggctgc gctggccgcg tgggagcgtg agcatccgca gcctagcgtc acgcttgcga
     2581 tggtcgccga ccatatcgag cacatcgtga agatcgcggg tgtcgaacat gtcggcatcg
     2641 gctcggactt cgacggcgca ggaaagacct tgcccgcggg gttgagtgac gtatcgacct
     2701 atcccgcgct gcttgccgaa ctgatgcgcc gtggatggag tgacggtgac atcgccaggc
     2761 tcgcgggtgg aaatattctg cgggtactcg atgaagcaga gtcggtcgaa cgatcgatga
     2821 atcgccgctg atgaccaggc gggacgacaa tgaccgctga tgtgtacgca aacgatgacg
     2881 gcttgcgcca cggcacaaga agccctcgac accaaggtgc agcgacgttc aatatcgtcg
     2941 ccgacgcgga cgcggatttg cttggacgca tcggcgccat tttgaacctc ctcaatgttg
     3001 cccctcgcag gttccacatg gaggctcgcg aggagggtac ggcgaccgtc aacgcgttgg
     3061 tcgattgcgc cgaattccag gcggatcttg tcgcccgcaa gctgcagcga cttaccagca
     3121 tccgcgacgt ggttgtggag tacacgccgg taacccaggg atgaagggtc atcacgatga
     3181 agaaactgaa acacgaaggt gagctgttca aggcggggct gctcgcagcg atgaaatacg
     3241 cgcaagagcg cggcgccgtc gaattcgagc cgacggattc cgcgagccag aagttgctct
     3301 acgtttaccg gctcctggtg cacgacaaga tgatccagcc gctgcccgaa gatcaggtcg
     3361 ccgagaacac catgaggcac aagctggcaa tctggtattc gaagcagctc ccggcggatc
     3421 atccgctgct gaagtgactg accgaggcgc aaacccgatc attcattggt tcgaagacgg
     3481 caaggcattc gaggcgcagt ggcgctcgca tagcgatgca ccgcctgccg cgcgcgtcgt
     3541 tgtcgctgac gggaagatga cagcagacga ggcctatggt ctcgcgtgcc aggggaccgc
     3601 acttctctgg cgaggtgact ttcaagaggc gcgccagatg ttgagtgcgt tggggaatcg
     3661 cgcggatcgt ccgcagcgtc gatcgcgaaa ttcagccgca acttcagtcg caaattcatt
     3721 ggccagcccg gccgacgcgt tccatctgta ccggcaagcc aggtcgcaac gggcccgcac
     3781 gctcaatgca ttgttgattc cgctcaacga cgactactcg atcccgttgc gacgagcgcc
     3841 agacatccgg caagcctgcg cggaagccta cggtcccgcg gagtccgcat cagtcacatc
     3901 gctgcgagcg ttgctcggtg tgatcggtgc gcacgagtgg cgcacgaaag gcatcgacat
     3961 tccggaggca ggcggccgaa tccatccgca cttcggtgtc ttcgcaccga tccgccgtga
     4021 gtacgtggac ctcgtcagca cgacgcctct gccggccgcg tgcgtttcat ccaatgttgc
     4081 gttcgatatc ggcacgggca ctggcgtgct cgccgcggtg ctggcacggc gtggcatcga
     4141 atgcgtgatc gctactgacc tcgatccacg tgcactggca tgcgcgcgtg agaacgttcg
     4201 gcagctcggg ctcaccgatc gcatcgatgt cgtgcaggcg gatctctttc ccgagggtcg
     4261 cgcggcgctg gtgatctgca acccgccctg ggttcctgcg cggccgagct cgcccatgga
     4321 aaacggaatc tatgatccgg aaagccagat gttgcgcgga ttcctgaaga aactgccggg
     4381 gcatctggag ccggggggtg aagggtggtt gatcctttcc gacctggcgg agcatctcgg
     4441 tttgcggacc agagc
//
LOCUS       DASQJS010000218         6751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3895364, whole genome shotgun sequence.
ACCESSION   DASQJS010000218 DASQJS010000000
VERSION     DASQJS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3895364"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3073)
                     /gene="gltB"
                     /locus_tag="VEW08_08885"
     CDS             complement(<1..3073)
                     /gene="gltB"
                     /locus_tag="VEW08_08885"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HYJ40891.1"
                     /translation="MTAIRLGPPAKQGLYDPQFEHDACGVGFIVDIKGRKSNAVVKQG
                     IQILKNLDHRGASGSEVNTGDGAGILLQMPHAFLKKVAKKDARINLPEPGLYAAGNIF
                     MPRNATQRRKIEEVFARVVQAEGQIYLGARSVPTDNSSLGETAKASEPFVRQVFIGRG
                     PDTTDEAEFERKLYVIRKRAYNEIRVSTIGGAEFWYIVSLSHKTLAYKGMLTTNQLDQ
                     YFLDLQNPLMESAIALVHSRFSTNTFPSWDRAHPYRCIAHNGEINTLRGNINWMRARE
                     ALFQSPAFGEDIRKITPIINPNGSDSSMFDNVLELMVLAGRSMPHAIMMMIPEPWSKH
                     ASMDPARRAFYQYHSSLMEPWDGPADIAFTDGKRIGAVLDRNGLRPGRYYVTKDDLVV
                     MASEAGVLEFPPEQIVRKGRLQPGRMFLVDTELGRIVEDEEIKAQVINERPYADWLKE
                     HLVHLKDLPTAPEMPLPEPVTLAKRQVSFGYTFEDQRILMAPMARDGNEGVGSMGNDT
                     PLAALSTKSRMLYDYFKQLFAQVTNPPIDCIREEIITSSDVWLGSEGNLLDPRPGDCR
                     RLELPGPVLTNEEFAKVRRISLPGLKVGTLSILFRAPLYGESGEDGLVRAIEEMRTEA
                     KRMIEEDEVNILILSDRGVNKEFAAIPSLLAVSGLHHYLIRQGLRMKVSLALETGDAR
                     EVHHFALLIGYGCSVINPYLAFETLDGMIRDDMLPNIEHKLACKNFAKAAVKGVVKVM
                     SKMGISAVQSYHGAQVFEAVGLRQDVIDEYFSGTASRVGGVGIDVIAKEVIMRHTMAF
                     AERKPQSQTELPSGGQYQWRSDGEFHLFNPESIHRLQKSVRTGSYSTYKSYAELIDDR
                     AKNLSTLRGLLDFKQGESVPIDEVESVESLMKRFKTGAMSYGSISKEAHETLAIAMNR
                     VGGKSNTGEGGEDPDRYVPMENGDSKNSAIKQVASGRFGVTSHYLVNAKELQIKMAQG
                     AKPGEGGQLPGTKVYPWIAKTRHTTAGVGLISPPPHHDIYSIEDLAELIHDL"
     gene            complement(3246..4133)
                     /locus_tag="VEW08_08890"
     CDS             complement(3246..4133)
                     /locus_tag="VEW08_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ40892.1"
                     /translation="MNPHRRALIELHACVVLWGFTAILGKLITMPAQALVWWRMLLVT
                     LALACFPRVWRGLAGIPPRLIAVYAGIGAVVAVHWLTFYGSIKLANASVAATCMALAP
                     AVTALIEPAITGARFERHNLLLGILVIPGVALVVGGIPGSMHLGFWVGVISAALASVY
                     NLLNKRYLSHHDAMAVTSIELGAGFLLVAALGPFVAPDGASVVLPSVQDGAWLLVLAI
                     LCTLIPFAVSLATLRHLSAFTAQLAINLEPVYAIGIAVLFLGEARELDGLFFLGVAIV
                     LAAVFGHGWLQTRDFRRTQ"
     gene            complement(4243..5517)
                     /locus_tag="VEW08_08895"
     CDS             complement(4243..5517)
                     /locus_tag="VEW08_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40893.1"
                     /translation="MKARALRWTFSVFAAAILGCDAAPSDPVERLRQVFAERPDVPYS
                     DNAFLDLLGFLGPAGVDPHELGIRRVVWLEKLDSHPEGAGTDPGTPTLDPKLYRSQAL
                     TQVIDACRDAIARACDSALDRVKGEAPLSHIESLLIARYDMLLSRRGWREIGVTQPAA
                     QRVPYEGAIEAQRFMLIRLRNVASAGDVESVRAVLTRDLTFWRTVLGSSDTLFSKMMA
                     LAAIRQHFTFGSFVLRDLPKARAMEAIPAQWRNEFTLRERSMLRPMAGELMIAEELLQ
                     TGKGKVLVSSEPEPDGALDKLSPRIYREPTISEIADTYLSVAEAFQVPLPEYEGVAAR
                     THGKHASDMGRGVSQYALRVGSAEGMRRAALLTVELRSQSVPVALLPERLQASPLRNP
                     YNGQPFAWDPADRAVVFIGPERRDFKRQAYPY"
     gene            complement(5564..6097)
                     /locus_tag="VEW08_08900"
     CDS             complement(5564..6097)
                     /locus_tag="VEW08_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40894.1"
                     /translation="MPDRRKPPADSGSRPGSRPDGAPLPSDTGGFSIETTTMRLKSLR
                     LRRAEVANKSVDEAVKTAIHNDPADVPSSGPSGRVRHDERGVAVWDWAVASGEFATLS
                     TTLALRKLEVADLKIEDHKPAELKLETTGRDKAGGFDPYNQRGTGKRQGEAAHRAGVT
                     GSANRGTVLDQLTGKKK"
     gene            complement(6202..6621)
                     /locus_tag="VEW08_08905"
     CDS             complement(6202..6621)
                     /locus_tag="VEW08_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40895.1"
                     /translation="MKFSTNVSLIFVAAILLAACGAKSSDEEQVRALISAVETAAEAR
                     DASDVLEHVADSYADSNGFDKTQLQNFLRGYFLAHPRIEVLVNIESLEFPVDGLAQAV
                     ISLATVELTDPDMQRLKVEFRRSGSAWQVVRADRVGR"
BASE COUNT         1186 a         2130 c         2168 g         1267 t
ORIGIN      
        1 tgaggtcgtg gatgagctcc gcgaggtcct cgatcgaata gatgtcgtga tgcggcggcg
       61 gtgagatcaa tcccacgccg gcggtggtgt gccgcgtctt cgcgatccac ggatacacct
      121 tggtgcccgg aagctgtccg ccctcgccgg gcttggcgcc ctgcgccatc ttgatctgca
      181 gctccttcgc gttgaccagg tagtggctgg tgacaccgaa gcggcccgac gccacctgct
      241 tgatggccga gttcttcgag tcgccgtttt ccatcggcac gtagcgatcc gggtcttcgc
      301 caccctcacc cgtgttgctc ttgccaccca cgcggttcat ggcgatggcg agcgtctcgt
      361 gcgcttcctt cgaaatggag ccgtaactca tggcgccggt cttgaaccgc ttcatgagac
      421 tttcgacgga ctcgacctcg tcgatcggca ccgactcgcc ctgcttgaag tcgagcaggc
      481 cgcgcagggt agatagattc ttcgcgcggt cgtcgatgag ctcggcgtag ctcttgtacg
      541 tggaatacga ccccgtgcgc accgacttct gcaggcggtg gatcgattcc gggttgaaca
      601 ggtggaactc gccatccgaa cgccactggt actggccgcc cgaaggcagc tcggtctggc
      661 tctgcggctt gcgctcggcg aacgccatcg tgtggcgcat gatcacttct ttcgcgatca
      721 cgtcgatgcc cacgccgccg acccgcgagg ccgtgccgga gaagtattcg tcgatcacgt
      781 cctggcgcag gcccacggct tcgaacacct gcgcgccgtg ataactctgc acggcggaga
      841 tgcccatctt ggacatgacc ttgaccacgc ccttgacggc ggccttcgcg aaattcttgc
      901 aggcgagctt gtgctcgatg ttcggcaaca tgtcgtcgcg aatcatgccg tcgagcgttt
      961 cgaaggcgag gtacgggttg atgaccgagc agccgtaacc gatgagcaat gcgaagtgat
     1021 gcacttcacg cgcatcgccc gtctccagcg ccagcgacac cttcatgcgc aggccctggc
     1081 ggatcaggta gtggtgcagg cccgagacgg ccagcagcga cggaatcgcc gcgaattcct
     1141 tgttcacgcc gcgatccgag aggatgagga tgttgacctc gtcctcctcg atcatgcgct
     1201 tcgcctccgt gcgcatttcc tcgatggctc gcacgagccc gtcttcgccg ctctctccat
     1261 atagtggggc gcggaacagg atgctcagcg tgcccacctt gagcccgggc agcgaaatgc
     1321 gccgcacctt ggcgaactct tcgttggtga gtaccggtcc cgggagctcg agccggcggc
     1381 agtcgcccgg acgcggatcc agcaggttgc cttcggagcc cagccacacg tccgaagagg
     1441 tgatgatctc ttcgcggatg cagtcgatgg gcgggttggt gacctgcgcg aacagctgtt
     1501 tgaagtagtc gtacaacatg cgcgacttcg tcgagagcgc ggccagcggc gtgtcgttgc
     1561 ccatggagcc cacgccttcg ttgccatcgc gcgccatcgg cgccatgaga atgcgctggt
     1621 cttcaaaggt gtagccgaac gacacctggc gcttggcgag cgtcaccggc tccggcaacg
     1681 gcatttccgg cgccgtgggc agatccttga ggtgcaccag gtgctccttc aaccagtcgg
     1741 cgtacggccg ctcgttgatg acctgcgcct tgatctcctc gtcttcgacg atgcggccga
     1801 gctcggtgtc cacgaggaac atgcgccccg gctgcagccg gcccttgcgc acgatctgct
     1861 cgggcgggaa ttcgagcacg ccggcttcgg acgccatgac gacgaggtcg tccttggtga
     1921 cgtagtaacg acccgggcga agtccattgc gatcgagcac cgcgccgatg cgcttgccgt
     1981 cggtgaaggc gatgtccgcg ggcccgtccc acggctccat caaactactg tggtactggt
     2041 agaacgcgcg gcgcgccgga tccatcgagg cgtgcttcga ccacggctcg gggatcatca
     2101 tcatgatcgc atgcggcatc gagcggccgg ccagcaccat gagctcgagc acgttgtcga
     2161 acatcgagga atccgaaccg ttcggattga tgatgggcgt gatcttcctg atgtcctcgc
     2221 cgaacgccgg cgactggaac agcgcctcgc gcgcgcgcat ccagttgatg ttgccgcgaa
     2281 gcgtgttgat ttcgccgttg tgcgcgatgc agcggtacgg atgcgcgcgg tcccagctcg
     2341 ggaacgtgtt ggtggagaag cgcgagtgca cgagggcgat cgccgattcc atcagcgggt
     2401 tctgcaggtc gagaaaatac tggtcgagct gattcgtggt cagcatgccc ttgtaggcca
     2461 gcgtcttgtg cgacagcgag acgatgtacc agaactccgc gccgccaatc gtggacacgc
     2521 ggatctcgtt gtacgcgcgc ttgcggatca cgtagagctt gcgctcgaac tccgcctcgt
     2581 cggtcgtgtc ggggccacgg ccgatgaaca cctggcgcac gaacggctcg gaggccttgg
     2641 cggtttcgcc cagcgaagag ttgtccgtcg gcacgctgcg cgcaccgagg tagatctgcc
     2701 cttcggcctg caccacgcgc gcgaacactt cttcgatctt gcggcgctgc gtcgcgttgc
     2761 gcggcatgaa gatgttgccg gccgcgtaca ggccgggctc gggcagattg atgcgcgcgt
     2821 ctttcttggc caccttcttg aggaaggcat gcggcatctg cagcaggatg cccgcgccat
     2881 cacccgtgtt gacctcgctg ccgctcgcgc cgcggtggtc gaggttcttg aggatctgga
     2941 taccttgttt gacgacggcg ttcgatttgc ggcccttgat gtccacgatg aagccgacgc
     3001 cgcaggcgtc atgctcgaac tgagggtcat aaaggccctg cttcgcgggg ggcccgagtc
     3061 gaatcgcggt catttttcag tcctccaatc acaaacgcgc ggggcgcaac ttccccgcgc
     3121 tatgggctcc gcgcccataa ctttcactct ttcaattggt tacatctgga ggcgcggccc
     3181 gcgaaggcgc ttcggcagtc agatatatat gagtctgatc atacagaccg acgctcgtcg
     3241 gtcaatcact gcgtacgacg gaaatcccgc gtttgcagcc acccgtggcc gaaaacggca
     3301 gcaagtacga tcgccacgcc caggaagaac aacccatcga gctcgcgcgc ttcgccgaga
     3361 aacagcaccg cgatgccaat ggcgtacacc ggctcgaggt tgatcgcgag ctgcgcggtg
     3421 aatgccgaca gatgccgcag cgtcgccagc gacaccgcga acggaatcag ggtgcaaaga
     3481 atggccagca ccagcagcca tgccccgtcc tggactgaag gcaggacgac gcttgcgccg
     3541 tccggggcca cgaatggacc gagcgccgcc accagcagga accccgcacc aagctcgatc
     3601 gaggtgaccg ccatcgcatc gtgatggctc aggtaacgct tgttgagcag gttgtacacg
     3661 gacgccaacg ccgcagagat gacccccacc cagaagccga gatgcatcga gccgggtatg
     3721 ccgccgacca ccagggcaac gcccggaatc accaggatgc cgagcagcag attatgtcgt
     3781 tcgaatctcg cgcccgtgat cgcgggctcg atgagcgccg tcaccgccgg ggccagggcc
     3841 atgcaggtgg ccgccaccga ggcgttcgcc agcttgatgg acccgtagaa cgtcagccag
     3901 tgcacggcca ccacggcgcc gatgccggcg tagacggcga tcaggcgcgg cggaattccc
     3961 gcgagcccgc gccacacgcg cgggaagcag gcgagcgcca gcgtgactaa caacatgcgc
     4021 caccacacga gtgcctgtgc cggcatcgtg atcagcttgc cgaggatcgc cgtgaatccc
     4081 cacagcacga cgcaggcgtg tagttcgatg agcgcgcgac ggtgcggatt catgtggcgt
     4141 aatctaaccg cagggaagag gaagctcgtg agttacgagg tggatgactt caccatcacg
     4201 acctcgcgca gcggaatcga caaaagaagt ggcagccgtg agtcagtacg ggtacgcctg
     4261 ccgcttgaag tcacgccgct cgggcccgat gaagacgacc gcccggtcgg cgggatccca
     4321 ggcgaacggt tgtccgttat agggatttcg cagcggcgag gcctgcagcc gttccggcag
     4381 cagcgcgacg ggcaccgact ggctgcgcaa ttccacggta agcagcgcag cgcgccgcat
     4441 tccttcggcg ctgccgacac gcaaagcgta ttggctgacg ccccttccca tgtcgctcgc
     4501 gtgtttgcca tgcgtcctgg ccgccacgcc ttcgtactcg ggaagcggta cctggaatgc
     4561 ttcggcgacg gatagatagg tgtccgcaat ttcactgatt gtcggctccc ggtatatgcg
     4621 cggcgacagt ttgtcgagtg cgccatcggg ctcgggttcg gaagacacca gcaccttgcc
     4681 tttgcccgtt tgcagcagtt cttcggcgat catgagttcg cccgccatgg gccgtagcat
     4741 ggagcgttcc cgcagagtga attcattgcg ccactgcgca ggaatcgcct ccatggcacg
     4801 cgcctttggc aggtcacgca atacgaagct gccgaaagtg aagtgctggc ggatcgcggc
     4861 cagcgccatc atcttgctga acagcgtgtc cgaagatccg agcaccgttc gccaaaaggt
     4921 gagatcgcgt gtcagcaccg cgcgaacgga ttcgacgtcg ccagccgacg ccacgttgcg
     4981 caggcggatc agcatgaaac gctgtgcttc gatcgctcct tcatacggca ctcgctgcgc
     5041 ggcaggttgc gtgacgccga tttcgcgcca gccgcggcga ctcagcaaca tgtcgtagcg
     5101 cgcgatcaac agggactcga tgtgcgagag cggtgcttcg cctttcacac ggtcgagtgc
     5161 gctgtcgcag gcacgtgcaa tggcgtcccg gcaggcgtcg atgacctggg tcaacgcctg
     5221 cgatctgtag agcttggggt cgagcgttgg agttcccgga tcggtgccgg cgccctcggg
     5281 gtgggagtcg agtttttcga gccacacaac gcggcgaatg cccagctcgt gcgggtcgac
     5341 ccccgcgggc cccaggaagc cgagcagatc gagaaacgcg ttgtcggagt acggcacgtc
     5401 aggccgctcg gcgaatacct gcctgagcct ctctaccgga tcggacggcg ccgcatcgca
     5461 gcccaggatc gcggcggcga agacggaaaa agtccatcgg agggcgcggg ctttcacgcc
     5521 gcaatcgtag ccgcaactgg agttggcgac taagcgccgc gaactacttc ttcttgcccg
     5581 tgagctgatc gagcacggtg ccgcggttcg ccgaacccgt gacgccggcg cggtgcgcgg
     5641 cttcgccctg tcgcttgccg gtgccacgct ggttgtaggg atcgaacccg ccggccttgt
     5701 cgcgcccggt ggtttccagc ttgagctcgg caggcttgtg gtcttcgatc ttcagatcgg
     5761 cgacctcgag tttcctgagc gcgagcgtgg tgctcagcgt ggcgaattcg cccgaggcca
     5821 cggcccagtc ccagactgct acgccacgtt cgtcgtgacg cacacggccc gaaggcccgc
     5881 tgctgggcac gtcggcggga tcgttatgaa tggcagtttt tacggcttcg tccacgctct
     5941 tgttcgcgac ctcggcgcgg cgcaggcgca gcgacttgag ccgcatggtg gtcgtctcga
     6001 tgctgaagcc gccggtatcc gacggcagag gcgcgccatc aggccggctg cctgggcggc
     6061 tgccgctgtc tgcgggtggt ttgcggcggt cgggcatcgg attctcgcga acgaaatgac
     6121 ttcagtctca cagacagttg tcggcacgca ctggctcgga ttgagcgtca aaaggtgatg
     6181 tttttcacgt tttgacgtag gtcagcgtcc gacacggtcg gcgcgcacta cctgccacgc
     6241 gcttcccgaa cggcggaact ctaccttcag acgctgcata tcgggatccg taagttccac
     6301 cgtggcgagg gagatgaccg cctgggccag cccatctacc gggaattcca ggctctcgat
     6361 gttcaccagc acttcgatcc tgggatgcgc cagaaagtac ccgcgcagga agttctgcag
     6421 ctgggttttg tcgaagccgt tcgagtcggc gtagctgtcg gcgacgtgct cgagcacgtc
     6481 actcgcgtcg cgtgcttcgg cagcggtttc gacggcgctg atcagcgcgc gcacctgttc
     6541 ctcgtcgctc gatttcgctc cgcacgcggc aagaagaatc gccgcgacga aaatcaggga
     6601 cacgttcgtg gaaaatttca tggcccgtcc tattgcagtt gaagtcagtt cgccctatcg
     6661 ttcgcgcgcg ttgcggggtt gggaaccggt cgtgacgtcc agaaatggtg ccccggaccc
     6721 agttggaggt ggaccactta aatgaagaag c
//
LOCUS       DASQJS010000219        10059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3580405, whole genome shotgun sequence.
ACCESSION   DASQJS010000219 DASQJS010000000
VERSION     DASQJS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10059
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3580405"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VEW08_08910"
     CDS             complement(<1..323)
                     /locus_tag="VEW08_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40896.1"
                     /translation="MANSKKTRPRASLLGQNLRTLQPKLRMIANGDANVNAIRAEMSA
                     AVKVTNSRLLDKIPQIRGEGHEALSKADFREVFKRKASRPTHLKMLTGQVMTNVFIHT
                     TTSDAL"
     gene            442..828
                     /locus_tag="VEW08_08915"
     CDS             442..828
                     /locus_tag="VEW08_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HYJ40897.1"
                     /translation="MQIQINTDHNIHGYESAAASVRATVENALRRFSTQITRVDVHLG
                     DENAGKRGGDDKRCMMEARLEGHQPVAVTHHAATVVQAIDGAAEKMAHRLERMLGRLH
                     SHRGNGAMPVFAEPDVPGLPDNERPA"
     gene            complement(829..1338)
                     /locus_tag="VEW08_08920"
     CDS             complement(829..1338)
                     /locus_tag="VEW08_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40898.1"
                     /translation="MRAETHGGSGVDVRLWPMTATLRKDFDEFLFAPVGKDSDGSPVT
                     LLTVLARLGVDPWQEASDLAHLSREPAMQRLASRLEAMPNGPATAADTVTIATRLIAL
                     LHRVPTRAAPSSPEAPLQAKVLMPVKGISPAFFVLVGLIFMLVAQCALTTTHGPAPMD
                     TTLSRDSEK"
     gene            complement(1401..1718)
                     /locus_tag="VEW08_08925"
     CDS             complement(1401..1718)
                     /locus_tag="VEW08_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40899.1"
                     /translation="MKISKSLIAATVIASLPLAAFAGDKDKTVTPDGAATSAQFDTLD
                     TNRDGRISRVEATSDSKIVFSTADKDGDGYLDNNEFTHRSMTMEPMPTSGNPATDSEQ
                     PRK"
     gene            complement(2085..2390)
                     /locus_tag="VEW08_08930"
     CDS             complement(2085..2390)
                     /locus_tag="VEW08_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40900.1"
                     /translation="MKLSRSIIAAAVVATMPLATFAGEKDKTAAPAATSSQFSALDTN
                     RDGRLSPAEAANDTKIVFSTADKNGDGYLDNDEYTHLDRSSETMPTHPATDKPAPPK"
     gene            complement(2518..3201)
                     /locus_tag="VEW08_08935"
     CDS             complement(2518..3201)
                     /locus_tag="VEW08_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40901.1"
                     /translation="MNTRFIAVLALLLPTVAIAQTARYPLTSPANLRPHNVVVEPATL
                     HGKKGLKVVAAQLTVPAGTTVETLALITGLEFGDGVIEAEIAGDVLPGAPEGARGFVG
                     IAFRVQDDLKTYDAFYLRPTNGRADDQVRRNHSAQYISHPDWPWFRLRKEFPSRYESY
                     VDLVPGTWTKVKIEVRGEQARLYVHAMEQPALIVNDVRSGANGKGAVALWIDVGTVAH
                     FRNLSVSKK"
     gene            3301..4173
                     /locus_tag="VEW08_08940"
     CDS             3301..4173
                     /locus_tag="VEW08_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HYJ40902.1"
                     /translation="MKTLKCGLIQMGLKGDVSLGPEKIRDKMIEAHLPLIDKAGAAGV
                     QVLCFQEIFTQPYFCPSQDRKWYAAAEAVPDGYTTRLMQEYAKKYKMVMVVPVYEEAM
                     TGVYYNTAAVIDADGKYLGKYRKTHIPQVDPGFYEKFFFKPGDLGYPVFQTAYVKLGV
                     YICYDRHFPEGWRALALNGAEYIVNPSATVAGLSKYLWELEQPASAAANGVFIGAINR
                     VGKEEPWAGKMGEFYGSSYIVNPRGEIEAQASYDKDELLIHEIDLDMIRKVRDTWQFF
                     RDRRPDAYGALVQG"
     gene            4285..4851
                     /locus_tag="VEW08_08945"
     CDS             4285..4851
                     /locus_tag="VEW08_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HYJ40903.1"
                     /translation="MSGASSITDLIGQAQSGDGAAFDAIFKIAYDDLRDLASKRLRAS
                     GRDVFLDTTSLVHESFLRFTQAGRLNASNRAHFLNYAGCVMRSVIVDFARERLAQRRG
                     GKALHVTLDTGVSDSVSTGEEEVLRVHEALEELARHDEKLVRVVELRYFGGLSEAEIA
                     ESMGIHVRTVRRHWEKARLLLADALDFK"
     gene            4919..7867
                     /locus_tag="VEW08_08950"
     CDS             4919..7867
                     /locus_tag="VEW08_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYJ40904.1"
                     /translation="MIRWLQLESGGWDELSRLIDTALELPADARAQWVDSLDDTDLRN
                     RLRAILARSARVETSDFLGALPPLHATDTELASLNGETETAGDCVSGYRLVREIGRGG
                     MSTVWLAERTDGLIRRPVALKLPQGLWRRGTLADRFARERNILAALTHPNIARLYDAG
                     IGACGQPYLAIEYVEGQPIDAYCAAAQLSLHDRLRLFLSVARAVAYAHTQLIVHRDLK
                     PANILVTRAGEVRLLDFGIAKLLDPEQSGDAPQTELTAQAFTPDYAAPEQIAGEPVTT
                     ATDVYSLGVVLYELICEVRPYKLARGSRAALEHAVAGADPPRPSMAAGAVTWRKELRG
                     DLDTIALKALKKDPGERYATMSALADDIERYLDHRPVLAQPDTLGYRLAKFTRRNRAF
                     VAGIAVLTTAILASASISIWQARAARAEARHAAEIREFLASTIRDADPYRGEGRVLSA
                     ADMLRQARQRADSLRERPELRIAMLTLIAASLLNLEDFDAAESAAAQALTESIRTLGP
                     QHEQTLRARMTMIGVHRFRGRTADMRRELDEVDRSLATRSVVEPADRFFVLENRAHLA
                     IDAGEYETASATAKQALALAESAFGKRDPRTAAAAVLLAESYEYSEVTQEFALQAAER
                     AFQLTESVHGANAKHPRLITAREVYGRALGNAGQLASGIQQLETTLAHAIEVYGPTSS
                     SVGLLYSHIARYELRMGRLHPAIAHLDEAITILGTHAQRDSFTYLSPLGLRGTAQLNA
                     RNAAEALRDLEESERGYSKLFGAEHEETVIKSRYRSLALAYLGRIDEARAAIAPVLVL
                     YRTRYSDPLYLPSDTFVSAGIIERLAGNPEAARTLLDEALTTVGTSGNADRYRADVLA
                     QLGLTELDRARADPSLALRQLTEADGLQRKLNLIGPRRADVWLGLGRAHLTLGSNQLA
                     LEFLRQADEFWQEFDATNRWAGEAAAWHSRALAATGQRQRAREIGARALLVLRKSPLT
                     IDARLLDTIH"
     gene            7925..8866
                     /gene="cysK"
                     /locus_tag="VEW08_08955"
     CDS             7925..8866
                     /gene="cysK"
                     /locus_tag="VEW08_08955"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HYJ40905.1"
                     /translation="MIHDSILGTIGRTPIVRINRIAPAGTTMYVKCEFFNPLSSVKDR
                     LAIAIIEDAERTGVLRPGQTVIEATSGNTGIALAMVCAAKGYPFVAVMVETFSVERRR
                     VMRMLGASVVLTPAAERGQGMVRKAAELAQANGWFLARQFENPANPHYHRNTTGPEIL
                     QDFVGRKLHYFVSGWGTGGTLTGVGETLKLARPDVRVIAAEPESAQLLAGKPFAPHKI
                     QGWTPDFVPAVLNRAIADRIVPVSDSEAIEAARSLARNEGMLVGISSGATFAAALKVT
                     RDAGPGCVTLAMLPDTGERYLSTALFDGIADGSDTAL"
     gene            8926..9255
                     /locus_tag="VEW08_08960"
     CDS             8926..9255
                     /locus_tag="VEW08_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HYJ40906.1"
                     /translation="MKKNYLIIVSEDPLEFPATRRAGDLALQLKQCGHEVALFLVQNG
                     VLASRANASADGLSPIFASAIEVLADDFSLRERGIEFNELRAGVAPAPIDTVVSRLAA
                     GWNTLWS"
     gene            9258..9671
                     /locus_tag="VEW08_08965"
     CDS             9258..9671
                     /locus_tag="VEW08_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HYJ40907.1"
                     /translation="MTAKPCLTFLLMDPPFESARTTTAFRLIHSALAQGHDVKVFAYE
                     GAVSLAFARQAAHANGVHGRDAAQEAHPLTREWIAFLQADAGARGARLEWVNCGLCVD
                     ERGVGETVPEVRRGSPVDFLKMVKESVNTLVVPTR"
     gene            9680..>10059
                     /locus_tag="VEW08_08970"
     CDS             9680..>10059
                     /locus_tag="VEW08_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40908.1"
                     /translation="MQVLNIVDQAYRATVEEQDDTVLWFTQVLRGAEANVALLLRGSA
                     VNYLVFRQDAGGLAFGAWKQAQPPRVAASIVALLASHTPVLAVEEDLAERGIRTSDCI
                     AGVQAVPRAKLGEIVERFDRVWQW"
BASE COUNT         1824 a         3244 c         3198 g         1793 t
ORIGIN      
        1 agggcgtccg aggtcgtggt gtgaatgaac acattggtca tcacctggcc ggtgagcatt
       61 ttcaggtgcg tcggcctgga cgccttgcgt ttgaacactt cgcgaaagtc agccttcgaa
      121 agcgcctcgt gaccttcgcc gcggatttgc gggatcttgt ccagcagccg agagttggtg
      181 accttcaccg cggcgctcat ctcggcgcgg atggcgttca cgttcgcgtc gccgttggcg
      241 atcatgcgca gcttgggttg cagcgtccgc aggttctggc cgagcaagga cgcccgtggc
      301 cgggtctttt ttgagttcgc catgttctta tcctgtcacc agctcgccgt ttatctcacc
      361 cgctcatcga aaatgtgtag gaaggagccc cgaggcgccg gagtccattg tgcatggaag
      421 gcgcgggtgc gagtctgcag catgcaaatc cagatcaata cagaccacaa catccacggc
      481 tacgagtcgg cagccgcatc agtgcgcgcc acggtcgaga atgctctgag gcgcttcagc
      541 acgcagatca cgcgggtgga cgtacatctc ggtgacgaga acgccggcaa acgcggcggc
      601 gatgacaaac gctgcatgat ggaagcgcgc ctcgaaggcc atcagccggt ggccgtgacc
      661 caccacgccg caaccgtcgt gcaggcgatc gacggagccg cggaaaaaat ggcccaccgg
      721 ctcgagcgca tgttagggcg cttgcacagc caccgtggca acggcgccat gccggttttc
      781 gcggagccgg atgttccagg cctccccgac aacgaacgac cggcctgact atttctccga
      841 atcccgggaa agggtggtgt ccatcggcgc cggaccgtgc gtggtcgtca gcgcacattg
      901 ggcaaccagc atgaagatca gtccgaccag gacaaagaac gcggggctga tgcctttcac
      961 cggcatcaac actttcgctt gaagaggcgc ctctggagag gacggcgcgg ctcgcgtcgg
     1021 tacacgatgc agaagtgcaa tcagccgggt agcgatggtc accgtatcgg cggctgtggc
     1081 cggcccattg ggcattgctt cgagccgtga tgccagcctc tgcatcgccg gctcgcgcga
     1141 aagatgtgcg aggtcggagg cctcctgcca cggatcgaca ccgagtcgcg cgagcacggt
     1201 gagaagcgtc actggcgatc cgtcggagtc cttgccgacc ggagcgaaca ggaattcgtc
     1261 gaagtcctta cgaagagtgg cagtcatcgg ccatagtcgc acgtccactc cggatccacc
     1321 gtgcgtttcc gcacgcatga cgcccaatcg aaggcccgcg caaccaaggg gctgcgcggg
     1381 cttggctgcg tgaaatcaga ctatttgcgg ggctgctcgg agtccgtcgc cggattgccc
     1441 gatgtgggca tcggctccat cgtcatgctc cgatgcgtga actcgttgtt gtcgagatac
     1501 ccgtcgccgt ccttgtcagc ggtcgagaac acgatcttcg agtcgctggt tgcttcaacg
     1561 cgcgagatgc gcccgtcacg gttggtatcc agtgtgtcga actgcgcact cgtagctgcg
     1621 ccatcggggg ttacggtctt gtctttgtcg ccggcgaaag ccgccagcgg taacgacgcg
     1681 attactgtcg ccgcgatcaa cgatttggaa attttcatga attctcctga acattgcctg
     1741 gaaggtcgat aacgaattcg cgagttgaat ccggcgtttc gtcatcgtgt tctgttggag
     1801 tcgcgggagt tcgcttgggt tcgcgggttt gcgtggcgtt ttgccgccga atcgcgacat
     1861 tcgccatccg ggcgacgtcg atcggagaca gcgtcacggt gtctaactac aaaccgcgaa
     1921 cccgggggcc ggtgctgcga tcgtctcgcc gcgatggccg tgtcagacaa tgccgcggga
     1981 atctgcgtcc tgctgctttc cgcacgcatg gcccaggcga gagcccgcgc aaccaggcga
     2041 tcgcgcgggc gtaccaggct gttttccgtt cggatccggg ttgcttactt gggcggcgcc
     2101 ggtttgtccg tcgccggatg ggtcggcatc gtctcgctcg acctgtccag gtgcgtgtac
     2161 tcgtcgttgt cgaggtagcc gtcgccattc ttgtcagcgg tcgagaacac gatcttcgtg
     2221 tcgttggccg cttcggcggg cgagaggcgt ccatcgcggt tggtatccag cgcgctgaat
     2281 tgcgagctcg tcgcggctgg cgccgcggtc ttgtcttttt cgccggcgaa agtcgccaac
     2341 ggcatcgtcg cgacgactgc cgcggcgata atcgatctgg aaagtttcat gactatctcc
     2401 tcaatattgc cggagggttc atcacgccga gctgacactc ctcgcgtgac gggtcgtatc
     2461 ggacaatggc gcgtcgtgga cgcagactgc gccgccgcgc gcgagggcag aaatgtccta
     2521 cttcttcgaa accgaaaggt tgcgaaagtg agcgacggtt cctacgtcga tccacagcgc
     2581 gacggcaccc ttgccgttcg ccccactcct gacgtcgttg acgatcagcg ccggctgctc
     2641 catggcgtgc acgtacaggc gcgcctgctc gccacgaact tcgatcttca ccttcgtcca
     2701 ggttccgggc acgaggtcca cataggattc gtatctggac gggaattcct tgcgaaggcg
     2761 gaaccacggc cagtcgggat gcgaaatgta ttgggcggaa tgattgcgcc ggacctgatc
     2821 gtcggcgcgg ccgttggtcg gacgcaggta gaaggcgtcg tacgtcttga ggtcgtcctg
     2881 gactcgaaag gcaatgccga caaaaccccg cgcaccctcg ggcgcaccgg gcagaacgtc
     2941 accggcgatc tcagcctcga tcacgccgtc tccgaattcc aggccggtga tcagcgccag
     3001 tgtttccacg gtcgtgccgg cgggcacagt tagctgcgcc gccacaactt tcagcccctt
     3061 ctttccgtga agcgttgccg gctcgacgac cacgttgtga gggcgcaggt ttgcgggcga
     3121 cgtgagtggg tagcgcgccg tctgcgcgat cgccaccgtg ggcagaagaa gcgccagaac
     3181 tgcgatgaat cgggtattca tgcacctgaa tctaccaccc ggctgaggac cacgaaccgc
     3241 ccactgccgc gagatccgct cggctggtct cgttccccct ttttgtgttt ttatcggcac
     3301 atgaaaacac tcaagtgcgg attgatccag atggggttga agggcgatgt gtccctgggc
     3361 cccgagaaga tccgggacaa gatgatcgag gcgcatctgc ctctcatcga caaggccggc
     3421 gccgctggcg tccaggtact ctgcttccag gaaattttca cgcagcccta tttctgcccg
     3481 agccaggacc gcaaatggta tgcggcggcc gaagccgtgc cagacggcta cacgacgcgg
     3541 ctcatgcagg agtacgcgaa gaaatacaaa atggtgatgg tggtcccggt ctacgaggaa
     3601 gcgatgaccg gcgtgtacta caacaccgcc gcggtcatcg atgccgacgg caagtacctc
     3661 ggcaaatacc ggaagactca cattccgcag gtcgacccgg gcttctacga gaagtttttc
     3721 ttcaagcccg gcgatctggg ataccccgtg ttccagacgg cgtacgtgaa gctgggtgtt
     3781 tacatctgct atgaccgcca cttcccggaa ggctggcgcg cgctggcgct caacggcgcc
     3841 gagtacatcg tcaacccttc cgccaccgtc gcgggcctga gcaagtatct ctgggagctc
     3901 gagcagcccg cctcggccgc cgcgaatggc gtgttcatcg gcgccatcaa ccgggtcggg
     3961 aaagaagagc cgtgggccgg gaagatgggc gagttctacg gttccagcta catcgtcaat
     4021 ccgcgaggcg agatcgaagc gcaggccagc tacgacaagg acgagttgct gatccacgag
     4081 atcgacctcg acatgatccg caaggtgcgc gacacgtggc agtttttccg cgaccgccgc
     4141 cctgacgcct atggcgcgct cgtgcaaggc tgatccggag cgcgaacggc cgctcgaaac
     4201 tcaaaagggt atttccgcac aaatcacgga cttgggcagg ccgccgcgcc gtactttgtg
     4261 aactcgcggt cgtatgatcc gcgcatgagc ggtgcctcct ccattacgga cttgatcggg
     4321 caagcgcagt ccggcgacgg cgccgctttc gacgcgatat tcaagattgc ctatgacgat
     4381 ctgcgcgacc tcgcgagcaa gcgactgcgc gccagcggtc gggacgtgtt tctcgatacg
     4441 acctccctcg tccacgaatc cttcctgcgc ttcacacagg cgggccgcct caacgcgtcg
     4501 aaccgcgcgc atttcctgaa ctacgccggg tgtgtcatgc gctcggtcat cgtggatttt
     4561 gcccgcgagc gtcttgcgca acgtcgcggt ggcaaagcgc tgcacgtgac actcgacacg
     4621 ggcgtgagcg acagcgtgag cacgggcgaa gaagaggtgt tgcgcgtcca cgaagcgctc
     4681 gaagaactcg cccggcacga tgagaagctc gtgcgtgtgg tcgagttgcg ctacttcggc
     4741 ggcctgtccg aggcggagat cgccgaatcg atgggcattc acgtgcgcac ggtccgtcga
     4801 cactgggaga aagcccgcct gttgctggcg gacgcgctcg atttcaaata acgccgcgga
     4861 gacggctgtc ccatttgcct cgagctgtac gtattagcag tatggaggaa ctgcaaaagt
     4921 gatccggtgg cttcaactcg agtctggcgg ctgggatgag ctgagccggc tgatcgacac
     4981 ggcgctcgag ttgccggcgg atgcacgcgc gcagtgggta gactcgctgg acgacaccga
     5041 tctcaggaat cgcctgcggg cgatcctcgc gcgcagcgcc cgcgtggaaa ccagcgactt
     5101 cctgggcgcg ttgccaccgc tgcatgccac cgacaccgag ctcgcctcgc tcaacggtga
     5161 aacggagacc gccggcgact gtgtcagcgg ctaccggctg gtgcgcgaga tcgggcgcgg
     5221 cggcatgagc acggtatggc tcgctgaacg gaccgacggg ctcattcgcc ggccggtggc
     5281 gctcaaacta ccgcagggac tatggcgacg cggcacgctc gcggaccgat tcgcgcgcga
     5341 gcggaacatc ctcgcggcat tgacccatcc caatatcgcg cggctctacg atgccgggat
     5401 aggcgcctgc ggccagccgt acctcgccat cgagtatgtc gaaggacagc cgatcgatgc
     5461 gtactgcgca gccgcgcaac tttccctgca cgaccgcctg cgattgttcc tgagcgtggc
     5521 gcgggctgtg gcctacgcgc acacgcagct catcgtgcat cgcgacctga agcccgccaa
     5581 catcctcgtg acgcgcgccg gtgaggtacg actgctcgat ttcggcatag ccaaactact
     5641 ggatcccgag caatcgggcg atgcgccgca aaccgagctc accgcccagg cctttacgcc
     5701 tgactacgcc gcgccagaac agattgcggg cgaaccggtg accaccgcca cggacgtcta
     5761 ttcgctcggc gtggtgctgt atgagctgat ctgcgaggtg cgtccgtaca agctcgctcg
     5821 cggttcgcgc gccgcgctcg agcacgcggt tgccggcgcc gatccaccgc ggcccagcat
     5881 ggccgcggga gccgtgacct ggcgcaagga gctgcgcggc gacctcgata cgattgcact
     5941 caaggccctg aagaaagatc cgggcgagcg gtacgccacg atgagcgcac tggccgatga
     6001 catcgagcgg tacctcgacc atcgaccggt acttgcacag cccgatacgc ttgggtaccg
     6061 gctcgcgaag ttcacgcggc gaaacagggc gttcgtcgcc ggcatcgccg tgctcacgac
     6121 ggccatcctg gcgagcgcca gcatttccat ctggcaggcg cgcgctgccc gcgccgaggc
     6181 acgccacgct gcggagatcc gggaatttct cgccagcacc attcgtgacg ccgatcccta
     6241 tcgcggcgag ggccgcgtcc tctcggctgc cgacatgctg cgccaggcgc gccagcgcgc
     6301 cgacagcctg cgcgagcgcc cggagctgcg tatcgcgatg ctcacgctga tcgccgccag
     6361 cctgctcaat ctcgaggatt tcgatgcggc ggaaagcgcc gcggctcagg cattgaccga
     6421 aagcatccgg acactggggc cgcagcacga acagacattg cgtgcccgca tgacgatgat
     6481 cggcgtgcac cggtttcgcg gacgcaccgc cgacatgcgt cgcgagctcg acgaagtaga
     6541 caggagcctc gcgacgcgtt ccgtcgtcga acccgcggac cggttcttcg tgctcgagaa
     6601 ccgcgcgcac ctggccatcg atgcgggcga atacgaaacg gcgagcgcga ccgcgaaaca
     6661 ggcgctggca ctggcggagt ccgccttcgg caagcgcgac ccccgcacgg ccgcggcagc
     6721 ggtgctgctg gcggaatcct atgaatactc cgaagtcacg caggagtttg cgttgcaggc
     6781 tgcggagcgc gccttccagc tgaccgaatc cgtgcacggc gccaatgcga agcatccgcg
     6841 cctcatcacc gcacgcgagg tctatggtcg tgcactggga aatgccggcc agctcgcctc
     6901 aggaatacag caactggaga cgacactcgc gcacgccatc gaggtctacg gccccacgag
     6961 ttcttcggtc ggactgctct actcgcacat cgcgcgctat gaattgcgca tgggtcggct
     7021 gcacccggcc attgctcatc tcgacgaggc catcaccatc ctcggcacac atgcgcagcg
     7081 tgattccttc acctacctga gtccgctcgg attgcgcggc accgctcaac tcaatgcgcg
     7141 caatgccgcg gaggcattgc gcgacctcga agagtccgag cgcggataca gcaaactctt
     7201 cggcgcggag cacgaggaga cggtgatcaa gtcgcggtat cgatcgctgg cgctggcgta
     7261 tctcggaaga atcgacgagg cgcgcgccgc gatcgcaccc gtcctcgtgt tgtaccgcac
     7321 gcgctacagc gacccgctct accttccgag cgatacattc gtcagcgccg gaatcatcga
     7381 gcggctcgcg ggcaatcccg aagcggcgcg taccttgctg gacgaagcgt tgactaccgt
     7441 cggcaccagc ggcaatgccg acagataccg cgccgacgtg ctggcgcaac tggggctgac
     7501 agagctcgat cgcgcgcggg cggatccttc gctggcgctg cgccaactca ccgaggccga
     7561 tggcttgcag cgcaaactca atctcatcgg cccgcgacgc gccgatgtct ggctgggatt
     7621 gggccgggcg cacctgaccc tggggtctaa ccagctcgcg ctcgaattcc tccgtcaggc
     7681 ggacgaattc tggcaggaat tcgacgcgac caatcgctgg gcgggcgaag ccgccgcctg
     7741 gcattcccgt gccctcgcgg cgaccggaca gcgccagcga gctcgcgaga tcggtgcacg
     7801 agcgctcctc gtgctgcgca aatctcccct gaccatcgac gcccggcttc tcgatacgat
     7861 tcattaacct gccccattcg cgtgcccgtt tccgtagtcc cggataggga ctcgagatgg
     7921 gcaaatgatc cacgacagca ttctcggtac gatcggccgc acgcccatcg ttcgcatcaa
     7981 tcgcatcgcg ccggccggca cgacgatgta cgtcaagtgc gaattcttca atccgctctc
     8041 ctccgtcaag gaccgcctcg cgattgccat catcgaggac gcggaacgca ccggcgtcct
     8101 caggccaggc cagaccgtca tcgaggcaac ctcgggcaat accggtatcg cactggcgat
     8161 ggtctgcgcg gcaaagggtt atcccttcgt cgcggtaatg gtggaaacgt tctccgtcga
     8221 gcgccgccgc gtcatgcgca tgctgggcgc gagtgtggtc ctcacgcccg ccgccgagcg
     8281 cggccagggc atggtgcgca aggcggcgga gctcgcgcag gcgaacggct ggttccttgc
     8341 gcggcagttc gagaatcccg ccaatccgca ttaccaccgc aacaccacgg gcccggaaat
     8401 cctgcaggat ttcgttggcc gaaaacttca ctacttcgtg agcggctggg gtacgggcgg
     8461 cacgctgacc ggcgtcggcg agacgctcaa gctggcacgc cccgatgtgc gcgtgatcgc
     8521 ggcggagccg gaatccgcgc agttgctggc cggcaagccg tttgctccgc acaagatcca
     8581 gggctggacg ccagacttcg tgcctgccgt gctcaaccgc gcgattgccg atcgaatcgt
     8641 gccggtgagc gacagcgaag cgatcgaagc cgcacgctcg ctggcccgca acgagggcat
     8701 gctggtcggc atctcgagcg gcgccacgtt cgcagccgca ctcaaggtaa cgcgcgacgc
     8761 aggtccaggc tgcgtcacgc tcgccatgct gcccgacacc ggcgagcgtt atctatctac
     8821 cgcgttgttc gatggcatcg ccgatggcag tgacaccgcg ttatgacacc cgtgcgtgcc
     8881 ccatcggccc atgaaaatcc gtagtcccgg acaggagcat gtgaaatgaa gaagaactac
     8941 ctgatcatcg tctccgaaga tccgctggag tttccagcca cgagacgcgc cggcgacctg
     9001 gcgctgcagc tcaagcaatg cggccatgag gtcgcgttgt tcctggtcca gaacggcgtg
     9061 ctcgcctccc gcgcgaacgc ttccgccgac gggctctcac ccatcttcgc atcggccatc
     9121 gaagtgctgg ctgacgactt ctcgttgcgc gagcgcggca tcgagttcaa cgaactgcgt
     9181 gcgggcgtcg cgcctgcgcc gatcgacacc gtcgtgagcc ggctcgccgc cggctggaac
     9241 accctctgga gctgatcatg accgcgaaac cctgtctcac gttcctgctg atggacccac
     9301 cgttcgagag cgcacgcacg accacggcgt ttcgcctgat ccactcggcg ctcgcccagg
     9361 gccacgacgt gaaagtcttc gcctacgaag gcgcggtcag tctcgccttc gcgcgccagg
     9421 ccgcgcacgc gaacggcgtg cacggacgcg acgcggcgca ggaagcgcac ccgctcacgc
     9481 gtgaatggat cgcgttcctg caggccgacg ccggggcacg cggcgcacgc ctcgaatggg
     9541 tgaactgcgg gttgtgcgtg gacgaacgcg gcgtgggtga aaccgtgccc gaagtgcgcc
     9601 gcggttcacc ggtcgatttc ctgaagatgg tcaaagagag cgtcaatacg ctcgtcgttc
     9661 ccacgcgtta ggagcagaca tgcaagttct caacatcgtc gatcaggcct accgcgccac
     9721 cgtcgaagaa caggacgaca cggtgctgtg gttcacgcaa gtcctgcgcg gcgcggaggc
     9781 gaatgtcgcg ttgctgttgc gtggcagcgc tgtcaactac ctggtgttcc gccaggacgc
     9841 gggtggtctc gccttcggag cctggaagca ggcccagcca ccacgagttg ccgcctcgat
     9901 cgtggcgctg ctcgcgagcc acactcccgt gctcgcggtt gaagaagatc tcgcggagcg
     9961 tggcatccgc accagcgact gcatcgccgg cgtgcaggcg gtgccccgcg cgaaactcgg
    10021 tgagatcgtc gaacgcttcg atcgcgtgtg gcagtggta
//
LOCUS       DASQJS010000220         8644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4523218, whole genome shotgun sequence.
ACCESSION   DASQJS010000220 DASQJS010000000
VERSION     DASQJS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8644
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4523218"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VEW08_08975"
     CDS             complement(<1..939)
                     /locus_tag="VEW08_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ40909.1"
                     /translation="MAANKFTGAVRRLKAVATKEVRQLSRDRVTFGMIVGVPLMQIIL
                     FGYAINYDVRNLATVVQDDANTSMSREFIAQLKATQVVHVTYTTHSSDELARLMREGR
                     ASMAVVIPRDFERRLQADTRPVVQILVDGSQPQLAVVAGGIAAVPLLTRHGEGPYSGG
                     KRMAPRRIEIRTLYNPEKRTAVQIVPALIGVILTMTMVLFTSGAIVRERERGNLELLI
                     ATPLSSLELMVGKLLPYVVIGLIQTTIILIVGAWLFSVPVVGRLVDLYAAALMFIAAT
                     LGLGLFVSTLAQTQFQAFQLAFLTLLPSILLSGFMFP"
     gene            complement(929..1870)
                     /locus_tag="VEW08_08980"
     CDS             complement(929..1870)
                     /locus_tag="VEW08_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ40910.1"
                     /translation="MNGPGQTAISARQLTRKFGDKVAVDHIDLDIPSGRIYGFLGPNG
                     SGKSTTLRMLCGMLLPSDGHAEVFGLSVVKDAEAIRRRLGYMPQKFSLWEDLTCDENL
                     RFMADLYGLEGDVGARIAEQRATYNLDGLRKQRAGTMSGGQKQRLALAAATLHSPELL
                     LLDEPTSAVDPQSRRDFWERLFQLADAGATILVSTHYMDEAERCHGLAIIAEGRMVAE
                     GTPRALMAGVEANIFEIEGADGSEAPRLIRELSWVHGVTQLGIRLRVLANRSVKDAEV
                     QLRTLLKQHGIDAQVELTDASLEDVFVVATRPEKLGR"
     gene            complement(1867..2829)
                     /locus_tag="VEW08_08985"
     CDS             complement(1867..2829)
                     /locus_tag="VEW08_08985"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ40911.1"
                     /translation="MTPRTLTGLAGLLLLTACGRHDESTLPGTLERDRVELVADANEV
                     IISLPFSEGATVKAGDVIVVQDRALSTADLDAARAQLAEAESRVEELRNGPRSTTIRA
                     GIARRDTARVQRDNAVRERDRLIGLVARSLVSKQEADRQSAAADAAESNLREAEADLR
                     ELQEGTRAEQVAQAREAADAARANLQGLETTSARLEVRSPIAGTIDALPFHLGEKPAR
                     GATVAVLLASTAPFARVHVPEPIRAQVKIGTPATLRIDGIERTFKGQVRFIASEAEFT
                     PYYSLTAADRSRLSFLAEVVIDDSGDASLPSGVPVDVTLELTPP"
     gene            complement(2826..3680)
                     /gene="nadC"
                     /locus_tag="VEW08_08990"
     CDS             complement(2826..3680)
                     /gene="nadC"
                     /locus_tag="VEW08_08990"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HYJ40912.1"
                     /translation="MKEGVAQAVIQCQVTAALAEDLGSGDVTAALVPASQQVRAQIIA
                     REPAILCGTDWAEATFRQLDPAIRLEWQARDGDHLRADLPVLKLSGPARPILSGERTA
                     LNFLQTLSGTATAASKYVEAVAGTGCRILDTRKTLPGLRLAQKYAARCGGAQNHRLGL
                     YDMVLIKENHIIAAGSIGSAIATARRTSPGIPVEVEVESLPEFDQACDAGADIIMLDE
                     LSLDDMREAVKRNRSRGARAKLEASGGVTLATVREIALTGVDYISIGGITKHVQAVDL
                     SMRFEFAA"
     gene            complement(3690..4301)
                     /locus_tag="VEW08_08995"
     CDS             complement(3690..4301)
                     /locus_tag="VEW08_08995"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HYJ40913.1"
                     /translation="MKIFTNHRYALVTGVVLALVLSYALHRCLMPDSEFNHLALARWV
                     HIVAGVFWIGLLYYLNTVQVPAMAAAAEDKGGPGPAAIGKYVAPRALFWFRWSALATW
                     VAGAWLLAERFVPAFTFGYSNGSHDYQTVVIGIGAWLGTIMFLNVWGVIWPNQKKILG
                     IKPATDEEKAKARKVALYASRTNFILSIPMLLCMAATSHGLPI"
     gene            4550..4876
                     /gene="grxD"
                     /locus_tag="VEW08_09000"
     CDS             4550..4876
                     /gene="grxD"
                     /locus_tag="VEW08_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HYJ40914.1"
                     /translation="MAEDVVSRIKTHIGTSPAVLFMKGTPDFPQCGFSAQVVAALQAS
                     DAEFHAVNIFEDPELRDALKKFSNWPTYPQLYVKGELVGGCDIVLDLHRSGELKKMLA
                     EAGSTA"
     gene            complement(4951..5664)
                     /gene="rnt"
                     /locus_tag="VEW08_09005"
     CDS             complement(4951..5664)
                     /gene="rnt"
                     /locus_tag="VEW08_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HYJ40915.1"
                     /translation="MEEILPVAASDEVLSAPLMSRRFRQFLPVVIDVETGGFNCVTDA
                     LLEIAAVMIEFGPDGKLRRGESHAYHVRPFEGANLEPASLAVNGIDPFHPLRPAIPEK
                     DALGRVFTEVRTAMKLQDCRRAVLVGHNAAFDLGFLNAAVARADIKRNPFHPFSCFDT
                     ATLAGAAFGQTVLAKAVQVCGISWDSSEAHSARYDAERTADIFCEVCNALDGLWRKSE
                     VRAQALWSGVTVPGGESGE"
     gene            complement(5664..6707)
                     /gene="pyrC"
                     /locus_tag="VEW08_09010"
     CDS             complement(5664..6707)
                     /gene="pyrC"
                     /locus_tag="VEW08_09010"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HYJ40916.1"
                     /translation="MEIEIRRPDDWHLHLRDGAAMAAVLPFTAERFARAIVMPNLKPP
                     VTTVAAAIDYRQRILAALPAGSAFTPLMTLYLTDQTNPAEIDKALKSHSVHACKLYPA
                     GATTNSDSGVTDIRRIDDVLARMSDAGMPLLVHGEVTARQVDVFDRESRFIDDVLIST
                     LARFPKLRVVFEHITTARAAEFVQRARDGVAATITPQHLLHNRNAIFEGGIRPHYYCL
                     PILKTEPDREALLAAATSGNPRFFLGTDSAPHAQHTKENACGCAGMFSAHAGIELYAE
                     AFEAVGKLDRLEGFASDFGADFYGLPRNADRIRLAKREWRPPASYPMGNSDKDGRLVP
                     MRANEAICWQLVS"
     gene            6897..>8644
                     /gene="aceE"
                     /locus_tag="VEW08_09015"
     CDS             6897..>8644
                     /gene="aceE"
                     /locus_tag="VEW08_09015"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HYJ40917.1"
                     /translation="MTEIMKLEDVDPTETREWIESIDSILKTQGAERAHFLLEQLIDY
                     TRRSGKYLPFRPNTAYVNTIPPGQEPSYPGNREFEKRIEACIRWNALAMVLNANKMSS
                     EYGGHLSTYASSATLYEVGFNHFWRAASAEHPGDMVFIQGHASPGIYARAFLEGRLSE
                     DQLKHFRQEASGDGLSSYPHPWLMPNFWQFPTVSMGLGPMMAIYQARFQRYMENREMV
                     PVSDRKIWAFLGDGEMDEPESMGALTLPVREKLDNLVFVVNCNLQRLDGPVRGNGKII
                     QELEAAFMGAGWNVIKVVWGSRWDPLLARDTQGLLQRLMEECVDGEYQNFKAKGGAYT
                     RENFFGKYPELKEMVANMSDDDIWRLNRGGHDSHKVFAAYSAAMNHKGQPTIILAKTV
                     KGFGLGKAAEGQMVAHQQKKLDEEALRAVRDRFNIPLTDEQVGKLSLVKPADDAPEMK
                     YLHEKRKALGGYLPARKIVAKPLVIPELSAFSAMLEGTGDREISTTMAFVRILTMLLK
                     DKNIGKNVVPIVPDEARTFGMEGLFRQIGIYSSAGQLYSPVDSDQLMYYKEDKKGQML
                     EEGINEAGSTCSWIAAG"
BASE COUNT         1588 a         2667 c         2847 g         1542 t
ORIGIN      
        1 agggaacatg aacccggaca gcaggatcga aggcagcagt gtgaggaaag cgagctggaa
       61 agcctggaac tgcgtttgcg ccagcgtcga gacgaagagt cccaggccca gcgtggccgc
      121 gatgaacatc agcgccgcgg catacagatc gacgagcctt cccacgaccg gcacgctgaa
      181 cagccaggcg ccgacgatca ggatgatcgt cgtctggatc aggccaatga cgacataggg
      241 gagcagcttg cccaccatga gctcgaggct gctgagcggc gtggcaatca gcaattccag
      301 gttgccgcgc tcacgttcgc gaacgatggc gcccgaggtg aacaacacca tggtcatcgt
      361 caggatcacg ccgatgagtg ccggcacgat ctgcactgcc gttcgcttct ccgggttgta
      421 gagcgtgcgg atctcgatgc gccgcggcgc catgcgcttg cctcccgaat atgggccttc
      481 gccgtgacgc gtgagcagcg gcaccgccgc gatgccaccg gccaccacgg cgagctgggg
      541 ttgcgaccca tccaccagga tctgcaccac cgggcgcgtg tcggcttgca gtcggcgctc
      601 gaaatcccgc ggtatcacga cggccatgct ggcgcggccc tcgcgcatga gccgcgcgag
      661 ttcgtcggag gagtgcgtgg tgtaggtcac atgaacgacc tgcgtggcct tgagctgcgc
      721 gatgaactcg cgcgacatcg aagtattcgc atcgtcctgc accacggtgg cgagattgcg
      781 cacgtcgtag ttgatggcat aaccaaacag gatgatctgc atgagcggta cgccgacgat
      841 catgccgaag gtcacgcgat cgcgtgaaag ctggcgcact tccttggtgg ccacggcttt
      901 cagccgccgc accgcaccgg tgaatttgtt agcggccaag tttttcgggc ctcgtcgcca
      961 ccacgaacac gtcctcgagg ctggcgtcgg tgagctccac ctgggcgtcg atgccgtgct
     1021 gcttcagcag cgtgcgcagc tggacctcgg cgtccttcac gcttcggttc gcgagcacgc
     1081 gcaggcggat accgagctga gtgacgccat gtacccacga aagctcgcgg atgaggcgcg
     1141 gcgcttcgct gccatccgcg ccttcgattt cgaaaatgtt ggcctcgaca ccggccatga
     1201 gcgcacgagg tgtgccttcg gccaccatcc ggccctcggc aatgatcgcc aggccgtgac
     1261 atcgttcggc ctcatccatg tagtgcgtgg acacgaggat cgtggcgcct gcatcggcga
     1321 gctggaacag ccgttcccag aaatcgcggc ggctttgcgg gtcgaccgcg ctggtgggct
     1381 catcgagcag caggagctcg ggggaatgca gcgtggcggc ggcgagcgcc aggcgctgtt
     1441 tctgtccgcc gctcatcgtg ccggcgcgct gcttgcgcaa cccatcgagg ttgtaggtgg
     1501 cgcgttgttc ggcgatgcgc gccccgacgt ctccttcgag tccgtacaga tcggccatga
     1561 aacgcaggtt ttcgtcgcag gtgaggtctt cccacagcga gaacttctgc ggcatgtagc
     1621 ccagccggcg ccggatggct tctgcatcct tgacgacgga aagcccaaac acttcggcgt
     1681 gaccatcact gggcagcaac atgccgcaga gcattcgcag cgtggtcgat ttgccggagc
     1741 cgttcggtcc gaggaatccg tagatgcgcc cggaggggat gtcgaggtcg atgtgatcca
     1801 ccgcgacctt gtcgccgaat tttcgcgtga gctgccgcgc ggaaattgcc gtctggccag
     1861 ggccattcac ggcggcgtca gctccagtgt gacatccacc ggcacgccgg aaggcagcga
     1921 tgcgtcgccg gaatcgtcga tgaccacttc ggccaggaag gagaggcgcg aacgatccgc
     1981 cgcggtgagt gagtagtaag gcgtgaactc cgcttcactg gcgatgaaac gcacctggcc
     2041 cttgaacgta cgctcgatgc cgtcgatgcg cagcgtcgcc ggcgtgccga tcttcacctg
     2101 cgcgcggatg ggttcgggca catgcacgcg cgcaaacggc gcggtggaag ccagcagcac
     2161 ggctacggtg gcgccgcgtg cgggtttttc gccgagatga aaggggagtg cgtcgatggt
     2221 tccggcgatc ggcgagcgta cctcgagccg cgcggaagtc gtctccagtc cctgcaggtt
     2281 ggcgcgcgca gcgtcggcag cctcgcgggc ctgcgcgacc tgctcggcgc gcgtgccttc
     2341 ctgcagctcg cgcaggtctg cttcggcttc gcgcaagttc gactcggcgg cgtcggcggc
     2401 tgccgattgc cggtccgctt cctgcttgct cacgaggctg cgtgccacga ggccgatcag
     2461 tcggtctcgc tcccgcaccg cgttgtcacg ttgcacgcga gcggtgtcgc gacgcgcgat
     2521 gccggcgcga atcgtggtgc tgcgcgggcc gttcctgagt tcttcgacgc gcgattcggc
     2581 ctcggcaagt tgcgcacggg cggcgtcgag atcggcggtc gacagcgcgc gatcctgcac
     2641 cacgatgaca tcgccagcct tgaccgtcgc gccttcggag aacggcagcg aaatgatgac
     2701 ttcgttcgcg tccgccacga gctccacgcg atcgcgctcc agcgtgccgg gcagcgtgct
     2761 ttcgtcgtgg cgtccgcacg cggtgaggag gagcaagccc gcgaggccgg tgagggtacg
     2821 cggcgtcacg cggcgaactc gaatcgcatg gacaggtcga ccgcctgcac gtgtttggtg
     2881 atgccgccaa tggagatgta gtcgacgccg gtgagtgcga tctcgcgcac ggtggccagc
     2941 gtcacgccac cggaggcttc gagtttcgcc ctggcgcccc gggaacgatt gcgcttcacg
     3001 gcttcccgca tgtcatcgag ggaaagttca tcgagcatga tgatgtcggc gccggcgtcg
     3061 caggcctggt cgaattccgg cagggattca acttccactt cgaccgggat gcccggagaa
     3121 gttcgtcgtg ccgttgcgat ggcgctgcca atggagcccg cggcgatgat gtggttctcc
     3181 ttgatgagca ccatatcgta tagacccagg cgatggttct gcgcgccgcc gcatcgcgcg
     3241 gcgtatttct gcgcgagccg cagacccggc agcgtcttgc gcgtgtcgag gatccggcag
     3301 cccgtgcccg cgaccgcttc gacatatttg ctggccgcgg tggcagtacc ggaaagcgtc
     3361 tgcaggaaat tgagcgccgt gcgttcgccc gaaaggatgg ggcgcgccgg acccgaaagt
     3421 ttgagcaccg gcaggtcggc gcggaggtga tcgccatcgc gtgcctgcca ttcgaggcgg
     3481 atggccggat cgagttgccg gaacgtggct tccgcccagt ccgtgccgca gagaatcgca
     3541 ggttcgcgcg caatgatctg cgcgcgaacc tgttgtgagg cgggaaccag cgcggcggtg
     3601 acgtcgcccg aacccaagtc ttcagccagc gcggcagtga cctggcactg aatgactgct
     3661 tgcgcgaccc cttctttcaa actaggcgat cagatgggca agccgtgtga cgtggccgcc
     3721 atgcacagca gcatcggaat ggagaggatg aagttggtac gcgaggcata caacgccact
     3781 ttgcgcgcct tggccttttc ttcgtcggtt gccggcttga tgccgagaat cttcttctgg
     3841 ttaggccaga tcacgcccca cacattgagg aacatgatgg tgccgagcca ggcgccgatg
     3901 ccaatgacca cggtctggta gtcgtgtgaa ccgttcgaat agccaaacgt gaatgccggc
     3961 acgaagcgct ctgcgagaag ccaggcgccc gcgacccagg tggcaagcgc ggaccagcgg
     4021 aaccagaaca gcgcgcgcgg cgcgacgtat ttgccaatgg cggcggggcc gggcccgccc
     4081 ttgtcctcgg cggcggcggc catcgccgga acctggacgg tgttcaggta gtacagcagg
     4141 ccgatccaga acacaccggc cacgatgtgt acccagcgcg cgagcgccag atggttgaat
     4201 tcggagtccg gcatcaggca gcgatgcagg gcgtaggaca gcaccaatgc caggacgacg
     4261 cccgtgacca gtgcgtagcg atgattcgtg aatattttca ttgtcttaaa tctccccggt
     4321 gagcggtttg aagaataggg gacgagcccc cataattcaa cgcgctccgc gtgggccgga
     4381 catcatgatt gaacgcccgc catcaccctg catcaaagtc tgtgtactgg tcgccggcga
     4441 ccgctgcgcg ggatgtttgc gtacggtgga tgaaatcgcg gcgtggccgg caatgagcgc
     4501 ggaagcgcaa tgggcgctcc ttggggcgct ggatcaaagg agaaggaaag tggcagaaga
     4561 cgtagtcagt cgcatcaaga cccatatcgg aaccagtccc gcggtgctgt tcatgaaggg
     4621 caccccggat tttccgcagt gtggtttttc ggcgcaggtc gtcgccgcgc tgcaggcttc
     4681 ggatgccgag ttccatgcag tgaacatctt cgaggacccg gagctgcgtg acgcgctcaa
     4741 gaagttctcg aactggccta cctacccgca gctgtacgtg aagggtgagc tggtgggcgg
     4801 atgcgacatc gtgctcgacc tccatcgcag cggcgagctc aagaagatgc tcgctgaagc
     4861 gggttccacg gcctgaaatt gttggtgccg ggtgggattg gagtccggca actagacgag
     4921 gtgcgtatgg tggttcagta gctcggcacg ctactcccca ctttccccac ccggcaccgt
     4981 gactccgctc cacaacgcct gcgcgcgcac ttccgatttg cgccacaagc cgtcgagcgc
     5041 attgcacact tcgcaaaaga tgtcggcggt gcgttcggcg tcgtagcgcg ccgaatgcgc
     5101 ttcactcgaa tcccaactga tgccgcacac ctgcaccgct ttcgcgagca cggtctgccc
     5161 gaacgcggcg ccggcgagcg tcgccgtgtc gaaacaggaa aacgggtgaa acgggttgcg
     5221 cttgatgtcg gcgcgcgcca cggcggcatt gagaaacccg aggtcgaagg cggcgttgtg
     5281 cccgaccagg acggcacgac gacaatcctg caatttcatt gcagtgcgca cctccgtgaa
     5341 aacgcggccc aacgcgtctt tttccgggat cgcgggtcgc agaggatgaa aagggtcgat
     5401 gccgttcacc gcgagagatg cgggctcgag attggcgcct tcgaatggcc gcacgtgata
     5461 ggcgtgcgat tcgccgcggc gcagttttcc gtccgggccg aactcgatca tgaccgcggc
     5521 gatttcgagc agcgcgtcgg tgacgcagtt gaaacccccg gtttcgacat cgatgaccac
     5581 cggaaggaac tggcggaagc gccggctcat gagcggtgcg ctcaggacct cgtcacttgc
     5641 ggcaacgggc aggatttctt ccatcagctc accaactgcc agcagatggc ttcattggcg
     5701 cgcatgggca cgagccggcc gtccttgtct gaattgccca tcgggtagct agccggcggc
     5761 cgccactcac gtttcgcgag gcgaatgcga tcggcgttgc gcggcagccc gtagaaatcg
     5821 gcgccgaaat cggacgcaaa cccctccagc ctgtcgagct tgcccacggc ttcgaaggct
     5881 tcggcataca actcgatgcc cgcgtgcgcg gagaacatgc cggcgcatcc gcaggcgttc
     5941 tccttggtgt gctgtgcgtg gggcgcgctg tcggtgccga gaaaaaaacg cgggttgccg
     6001 ctggtggccg ccgccagcag cgcttcgcga tccggttcgg tcttgagtat cggcaggcag
     6061 tagtagtgcg gtcgtatgcc accttcaaag atggcattgc ggttgtgcag caagtgctgg
     6121 ggcgtaatcg tggccgcgac gccgtcgcgc gcgcgttgga cgaattcggc agcgcgcgcc
     6181 gtggtgatgt gttcaaagac cacgcgcaat ttcggaaacc gggcgagcgt ggaaatcagc
     6241 acgtcatcga tgaaccggga ttcgcgatcg aacacatcaa cctggcgcgc ggtcacctcg
     6301 ccgtgcacca aaagcggcat gccggcgtcg ctcatgcgcg caagcacgtc atcgatgcgc
     6361 cttatgtccg tgacgcccga atccgagttg gttgtcgcgc cagccggata gagcttgcag
     6421 gcgtggacac tgtgcgattt caacgccttg tcgatctcgg ccgggttggt ctgatcggtg
     6481 agatacagcg tcatgagcgg tgtaaaggcc gaccccgcgg gcagcgccgc caggatcctt
     6541 tgccgatagt cgattgcggc ggccacggtg gtgacgggcg gcttgagatt cggcatcaca
     6601 atggcgcgcg cgaagcgttc ggcggtaaac ggcagcacag ccgccatcgc cgcgccgtcg
     6661 cgcagatgca ggtgccagtc gtcggggcgg cggatttcga tttccactag tgatgggtcg
     6721 cgtaaaacgc gccgtaagct gctgtccgca ttgaggtttg cttgaccggg agaggcgaag
     6781 ccacgatcat atcgcgcctt gttttcccgc ccccctcacc tcattttcaa cgcggcgcat
     6841 gctagcacag cgcgcaccgt tctctttgct ttgccggccc ccggcagtgg atgcgtatga
     6901 cggaaatcat gaagctcgaa gatgtcgatc cgaccgagac acgcgagtgg atcgaatcga
     6961 tcgactcgat tctcaagacg cagggcgcgg agcgcgcgca tttcctgctc gagcaactca
     7021 tcgactacac gcgacgctcg gggaagtacc tgcctttccg tccgaacacc gcctacgtca
     7081 ataccattcc gcccggccaa gagccgtcct atccgggtaa ccgcgaattc gagaagcgca
     7141 tcgaggcctg catccgctgg aatgcgctgg ccatggtgct caatgccaac aagatgtcga
     7201 gcgaatacgg cggccacctg tccacctacg catcttcggc cacgttgtac gaagtggggt
     7261 tcaaccattt ctggcgcgcg gcgtcggcgg agcatcccgg cgacatggtg ttcatccagg
     7321 gccatgcctc gcccggcatt tatgcgcgcg cattcctcga gggccgcctg agcgaagacc
     7381 agctgaaaca ctttcggcag gaagcaagcg gtgatggtct ctcgtcgtat ccgcatccct
     7441 ggctcatgcc caacttctgg cagttcccga cggtgtcgat gggcctgggg cccatgatgg
     7501 ctatctacca ggcgcgcttt cagcgctaca tggaaaaccg cgaaatggtg cccgtgtccg
     7561 atcgcaagat ctgggcgttt ctcggcgacg gcgagatgga cgagccggag agcatgggcg
     7621 cgctcacgct gccggtgcgc gagaagctcg acaacctggt gttcgtcgtc aactgcaacc
     7681 tgcagcggct cgacggcccg gtgcgcggca acggcaagat catccaggag ctcgaggccg
     7741 cattcatggg cgcgggctgg aacgtcatca aggtggtctg gggttcgcgt tgggatccgc
     7801 tgctggcgcg cgacacgcag ggcctcctgc agcgtttgat ggaagagtgc gtcgacggcg
     7861 agtaccagaa tttcaaggcc aagggcggcg cgtacacgcg tgagaatttc ttcggcaagt
     7921 accccgagct caaggagatg gttgccaaca tgtcggatga cgacatctgg cgcctgaacc
     7981 gcggcgggca cgattcgcac aaggtgttcg cagcctactc ggcggccatg aaccacaagg
     8041 gccagcccac catcattctc gcgaaaaccg tcaagggctt cggcctcggc aaggcggccg
     8101 aggggcagat ggtggcccac cagcagaaga agctggacga ggaagccctg cgtgccgtgc
     8161 gcgaccgctt caacatcccg ctcaccgacg agcaggtcgg gaagctgtcg ctggtgaagc
     8221 cggccgacga cgcgccggag atgaagtacc tgcacgagaa gcgcaaggcg ctcggcggct
     8281 atctacccgc gcgcaagatc gtggcgaagc cgctggtcat cccggaactg tccgcgttca
     8341 gcgcgatgct ggaaggcacg ggcgaccgcg agatcagcac caccatggcc ttcgtgcgca
     8401 tcctcacgat gctgctcaag gacaagaaca tcggcaagaa cgtcgtgccc atcgtgcccg
     8461 acgaagcgcg caccttcggc atggaaggtc tgttccgcca gatcggcatc tacagctcgg
     8521 ccggccagct ctattcgccg gtcgattccg atcagctcat gtattacaaa gaggacaaga
     8581 aaggtcagat gctcgaagag ggcatcaacg aggcgggctc cacctgctcg tggatcgccg
     8641 ccgg
//
LOCUS       DASQJS010000221         4186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_756173, whole genome shotgun sequence.
ACCESSION   DASQJS010000221 DASQJS010000000
VERSION     DASQJS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756173"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VEW08_09020"
     CDS             complement(<1..699)
                     /locus_tag="VEW08_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ40918.1"
                     /translation="MNFHAVQAIYRFEMSRTWRTLFQSIATPVMTTSLYFVVFGSAIG
                     SRMMSIDGVSYGAFIVPGLIMLTVLMESVSNGSFGIYMPKFAGTIYELLSAPVGLTET
                     LLGYTGAAATKSVILGLVILVTARLFVPFSVEHPFWMVAFLVLTSVTFALLGFLIGIW
                     ADSWEKIQIVPMMVVTPLTFLGGSFYSISMLPPIWQKITLFNPVVYLVNGFRWSFYGK
                     SDVNVVLSFGMTLGF"
     gene            complement(722..1642)
                     /locus_tag="VEW08_09025"
     CDS             complement(722..1642)
                     /locus_tag="VEW08_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ40919.1"
                     /translation="MSVLTVSGLSKTYATGFHALKDVNLEIRRGEIFALLGPNGAGKT
                     TLINIICGIVTGSTGTVHADGHDIVREFRAARASIGLVPQELTTDMFETVWNTLTFSR
                     GLFGKPRDPAVIEKVLRDLSLWDKKDAKIMTLSGGMKRRVMIGKALSHEPTILFLDEP
                     SAGVDVELRRDMWEMVRKLRASGVTIILTTHYIEEAEEMADRIGVISKGSLIVVEEKT
                     ALMRKLGKKQLTLQLQAPLAAIPAGLAGLPLELAKDGTELVYSFDTQASETGIAGLLR
                     RLSELGIEFKDLHTAESSLEDIFVNLVRSR"
     gene            1770..2402
                     /locus_tag="VEW08_09030"
     CDS             1770..2402
                     /locus_tag="VEW08_09030"
                     /inference="COORDINATES: protein motif:HMM:NF018626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HYJ40920.1"
                     /translation="MRTLLEQFALAGLCLAASVAIRAGNADIVVAARSQVGVTTTYDP
                     AYRQLRYPSGDVPADRGVCTDVIVRALRAARAIDLQKQVHEDMKANWDAYPHPWRWRL
                     SKPDPNIDHRRVPNLMAYFRRLGYAVPLARAAADYLPGDIVAWDLGRGILHIGIVSDV
                     GVPAGPPFVLHNIGSGAQEEDILFRYTIVGHYRLPPPPVGERLRTPVATH"
     gene            2444..>4186
                     /locus_tag="VEW08_09035"
     CDS             2444..>4186
                     /locus_tag="VEW08_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ40921.1"
                     /translation="MVAKPSIAKQADVHGEALNSPRQFFGVFRYSKRALELLWTTSRP
                     LAFFLGLMTILAGVLPSGVAWVGARIVDSVVDATRSHADGAEVDIVPVLLWVLAEALI
                     LVFITAAHRGITFCSALLKAKLSNRVNVMILEKALTLELTHFEDSEFYDKLTRARREA
                     SSRPLSLVMRTSGLFQNAIAITSFAVLLAGFSPWAVVVLIFGGLPAFLAETKFSGDAF
                     RLFRWRAPETRMQSYLETALAREDHAKEVKLFDLGKLFLGRYKAIYEGLYEKDRNLTV
                     RRDAWGFAFTLVSIAALYSAYAWCAAEAVRGLITLGEMTMYIMLFRQGQAAVTASLGA
                     IGGMYEDNLYLSTLYEYLEQPVDESRGTAVSGPDPTAGVQFEDVEFRYPGSDKVAITG
                     INLQIKRGESLALVGENGSGKTTLIKLLTRLYKPTHGRVLLDGRDLQEWDTTILRRRI
                     GVIFQDFVRYQLMVGENIGAGDVRAFEDEERWHEAAVSGKAAPFIGEMPLQYHTQLGK
                     WFKDGRELSGGQWQKIALSRAFMRSDADILVLDEPTAAMDAAAEAEVFEHFRSLMGNR
                     IAIVISHRFSTVRMA"
BASE COUNT          751 a         1319 c         1349 g          767 t
ORIGIN      
        1 gaagccgagc gtcatgccga agctcaggac gacgttcacg tcggatttgc cgtagaagct
       61 ccagcggaag ccgttcacca ggtacaccac cgggttgaac agcgtgatct tctgccagat
      121 cggcggcagc atcgagattg agtagaagct gccgccgagg aaggtgagcg gcgtaaccac
      181 catcatgggc acgatctgaa tcttctccca gctgtcggcc cagatgccga tcagaaagcc
      241 cagcagcgcg aacgtgaccg acgtgagcac caggaacgcg accatccaga acggatgctc
      301 gacggagaag ggcacgaaca ggcgtgcggt caccaggatc accaggccca ggatcaccga
      361 cttggtggcc gcggcgcccg tgtagcccaa caaggtctcg gtgagcccca ctggcgccga
      421 cagcaactcg tagatggtgc cggcgaactt gggcatgtag atgccgaagg agccgttgga
      481 cacgctctcc atgagcaccg tcagcatgat gagccccggc acgatgaacg cgccgtagct
      541 caccccgtcg atggacatca tgcgtgagcc gatggccgaa ccaaacacga cgaagtacag
      601 cgaggtggtc atgaccggcg tggcgatgct ctggaacagc gtgcgccagg tgcgcgacat
      661 ctcgaagcgg tagatagcct ggaccgcgtg gaagttcatg gtcgcgccct cgaattgttc
      721 attaccgtga tctcacgagg ttcacgaaga tgtcctcgag cgagctctcc gcggtgtgca
      781 ggtccttgaa ctcgatgccg agttcactga gcctccgcag cagcccggcg atgccggttt
      841 cgctggcctg ggtatcgaag ctgtagacga gctcggttcc gtccttcgcg agctcgagcg
      901 gcaggccggc gagccccgcg ggaatggctg ccaggggcgc ctgcaattgc agcgtgagct
      961 gcttcttgcc cagcttgcgc atgagcgccg tcttctcctc caccacgatc agcgagccct
     1021 tgctgatcac gccgatgcga tcggccatct cctcggcttc ttcgatgtag tgcgtagtaa
     1081 ggatgatggt gacgccgctg gcgcgcagct tgcgcaccat ctcccacatg tcccggcgaa
     1141 gctccacgtc cacgcccgcc gacggttcgt cgaggaacag gatggtgggt tcgtgcgaca
     1201 gcgccttgcc gatcatgacg cggcgtttca tgccgcccga cagcgtcatg attttcgcgt
     1261 ccttcttgtc ccacaacgac aggtcgcgca ataccttttc tatgacggcc ggatcgcgcg
     1321 gcttgccgaa caggccgcgg ctgaacgtga gcgtgttcca caccgtctcg aacatgtcgg
     1381 tggtgagctc ctgcggcacg aggccgatgc tggcgcgcgc cgcgcggaac tcgcgcacga
     1441 tatcgtggcc gtcggcatgg acagtgccgg tggacccggt gacgatgccg cagatgatgt
     1501 tgatgagcgt ggtcttgccg gcgccgttcg gacccagcag cgcgaatatt tcgccgcggc
     1561 ggatttcgag attgacgtcc ttgagtgcgt ggaagccggt ggcgtaggtc ttggagagac
     1621 ccgagactgt gagtacggac atgggccgtg acgatacgga agcggccgct gcggctcaat
     1681 ggctcaatgg gggtagggcg gtgagatttg atgaatcgcg atcaaggcgc gcttgcctgc
     1741 caggcgatgc ctgcccgaga cttgcgtgcg tgaggacact gttagaacag tttgcgcttg
     1801 cgggtctatg tcttgcggca agcgtcgcga ttcgtgccgg aaatgcagac atcgtcgtcg
     1861 ccgcccgcag ccaggtcgga gtgacgacga cctacgaccc cgcctaccgg cagctgagat
     1921 atccgtcggg tgacgtgccc gccgaccgcg gcgtctgcac cgacgtgatc gtgcgtgcgt
     1981 tgcgcgcggc gcgcgcgatc gatctgcaga aacaggttca cgaggacatg aaggcgaact
     2041 gggacgccta tcctcatccg tggcgctggc ggctttcgaa gcccgacccg aacatcgatc
     2101 accggcgagt gccgaacctg atggcctact tcagacgcct gggttacgcg gtgccgcttg
     2161 cgcgcgcggc cgccgactat ctacccgggg acatcgtggc gtgggatctc ggtcgtggca
     2221 tcctgcacat cggcatcgtg agcgatgtcg gggtgcccgc cggaccgccg ttcgtattgc
     2281 ataacatcgg ctcgggcgca caggaagagg acatcctgtt ccgttacacg atcgttggac
     2341 actaccggtt gcctccgcca ccggtcggcg agcggttgcg gacacctgtc gccacacact
     2401 gacgcctttc cgcgtcgttt cacggtaatc ttcgcgcgca gacatggtcg ccaaacccag
     2461 catcgccaaa caagccgacg tccacggcga agccctgaac tcaccccggc agttcttcgg
     2521 cgtcttccgc tatagcaagc gggcgctgga gctgctgtgg acgactagcc ggccgctggc
     2581 atttttcctc ggactcatga cgattctcgc cggtgtgttg ccctcgggcg tggcctgggt
     2641 gggcgcacgc atcgtcgaca gcgtggtgga cgcaacgcgg tcgcacgccg atggagccga
     2701 agtcgacatc gtgccggtgc tgctctgggt gctggccgag gcgttgatcc tcgttttcat
     2761 caccgctgcg caccgcggca tcacgttctg cagcgcgttg ctcaaggcca agctctccaa
     2821 ccgcgtcaac gtcatgatcc tcgagaaggc gctgacgctc gagctcacgc atttcgaaga
     2881 ctcggagttc tacgacaagc tcacgcgcgc ccgccgtgaa gcgtcgagcc ggccactgtc
     2941 gctggtcatg cgaacgtcag gcctgttcca gaacgcaatc gcgatcacca gcttcgcggt
     3001 gctgctcgct ggcttctcgc cgtgggcggt ggtggtgctc attttcggcg gccttccggc
     3061 gttcctggcc gagacgaaat tctccggcga cgccttccgg ctgttccgct ggcgcgctcc
     3121 cgagacgcgc atgcagagtt atctcgaaac cgcgctggcg cgcgaggacc atgccaagga
     3181 ggtcaaactg ttcgacctcg gcaagctgtt cctcggccgg tacaaggcta tctacgaggg
     3241 gttgtacgag aaggaccgca atctcaccgt tcgccgcgac gcgtgggggt ttgcgttcac
     3301 gctggtgagc attgccgcgc tgtacagcgc ctatgcgtgg tgcgcggccg aggcagtacg
     3361 ggggctcatc acgcttggcg agatgaccat gtacatcatg ctgttcaggc aggggcaggc
     3421 cgcggttacg gccagcctcg gggccatcgg cggcatgtac gaagacaacc tgtatctctc
     3481 gacgctgtac gaatacctcg agcagccggt cgatgagagt cgcggcaccg ccgtcagcgg
     3541 cccggacccg accgccggcg tacagttcga agacgtggag tttcgctatc cgggttcgga
     3601 caaggtcgcg atcaccggca tcaacctgca gatcaagcgt ggcgagagcc tggcgctcgt
     3661 cggcgaaaac ggctcgggca agaccacgtt gataaagctc ttgacccgcc tctacaaacc
     3721 cacgcacggg cgcgtcctgc tcgacggccg cgatttgcag gagtgggaca ccaccatcct
     3781 gcgccggcgc atcggcgtca tcttccagga cttcgtgcgt taccagctca tggtgggcga
     3841 gaacatcggc gccggcgacg tacgcgcctt cgaagacgaa gagcgctggc atgaggcggc
     3901 ggtgtccggc aaggccgcgc cgttcatcgg agaaatgccg ctgcaatacc atacgcagct
     3961 cggcaagtgg ttcaaggacg ggcgcgagct ctccggtggg cagtggcaga aaatcgcgct
     4021 gtcgcgcgcc ttcatgcgca gtgacgccga catcctcgtg ctcgacgagc ccaccgccgc
     4081 catggacgcg gccgccgagg ccgaggtgtt cgagcacttt cgttcgctca tgggcaaccg
     4141 catcgccatc gtcatttcgc accgcttttc caccgtgcgc atggcc
//
LOCUS       DASQJS010000222         4455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_130683, whole genome shotgun sequence.
ACCESSION   DASQJS010000222 DASQJS010000000
VERSION     DASQJS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130683"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VEW08_09040"
     CDS             complement(<1..971)
                     /locus_tag="VEW08_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYJ40922.1"
                     /translation="MREWLQARHRGLFIRSYVVLAGGLLLVAVLLDLGFSALQSRQVR
                     AADPWLVSTFQLMESQLAAVPGSERAALAARLAEKLKLELDVLEAADISGAAPLESGP
                     RELTDDDGRIYFLWHSEALKGAIRLGPFEPARESLGVRFLPVLFYASILLIVGLWLRP
                     LLRDLRVLTVASQKFATDYREPLDTARHTTQLTSLATNLDDMSARVSQLIQSQKEMTA
                     ALSHEMRTPLARVRFAAAVLEGEVDEPLRAQLRAVNADVQQIDDLISDMLDYARLDHP
                     GLRMSCQTVPLAPWLRQVLLSCPPHERRVNTVQQDGIETAWMEPRLM"
     gene            complement(968..1681)
                     /locus_tag="VEW08_09045"
     CDS             complement(968..1681)
                     /locus_tag="VEW08_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004651027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ40923.1"
                     /translation="MNTPSVDILLIEDDSRLAELTATYLEQNGLRVAVEERGDRAVER
                     FAREKPRLVLLDLLLPGKDGLSICRELRRVHDVPILILTARDTDIDHVIGLEAGADDY
                     VMKPVDPMVLLARVRALMRRAERATPSTGTHKPDLMLGALRISETSREVWLQSQPVPL
                     TTQEFELLSLLAHRAGELVSRDEVFRTMRGIDYDGLDRSIDGRVSKLRRKLGDDAAAP
                     TRIKTVWGKGYLLVPDAWE"
     gene            complement(1678..2082)
                     /locus_tag="VEW08_09050"
     CDS             complement(1678..2082)
                     /locus_tag="VEW08_09050"
                     /inference="COORDINATES: protein motif:HMM:NF022895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3019 domain-containing protein"
                     /protein_id="HYJ40924.1"
                     /translation="MLARLTIFIAGGLGVAMTAAAADPLPVRLSVKPLLCVIDKDEVA
                     CTINFDVRWKSTLAAEYCLNDSARQTPLRCWPRTDSGEHSQERVVSEDFVFWLSPPAG
                     TERVAEAKIEVLRVGSDDRRRERRTRHVWDVL"
     gene            complement(2082..3008)
                     /locus_tag="VEW08_09055"
     CDS             complement(2082..3008)
                     /locus_tag="VEW08_09055"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HYJ40925.1"
                     /translation="MKTFRFQHLLAVAALWSCAQLAAAQDAPATEAPPIPAASTPAPA
                     TTPPRPLPVEKNWRFGVALGYGERTNPLVQSDDIPVIVDLDIAWFGERWFFDNFDLGF
                     ELFDRPSFTTNLVARVNSDRAFFSKTNTKYINFSYLGGGVVGPAVDPGSGNLVEQPVK
                     FKPPKRDYAVEAGFEMLMDGEWGMAALRAFHDVSGTHHGYELSVDYGFRWVRGRWSIA
                     PNVGVSYKSAALSDYYWGVHADEATFLLPEYEAEGGVGWEAGLRTSYYLTKSVRIAVS
                     ANYERLQHSVAQSPLVEDKQVLGYFAGMAWQF"
     gene            3195..3455
                     /locus_tag="VEW08_09060"
     CDS             3195..3455
                     /locus_tag="VEW08_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40926.1"
                     /translation="MRRPINWFVFCLSLAFLFANVSLSRKASPPVELAAIPVTAELDF
                     SHVILAETTPPLFGYIRDDGSFMPLAQQSPKPSATSKVPARF"
     gene            complement(3456..4112)
                     /locus_tag="VEW08_09065"
     CDS             complement(3456..4112)
                     /locus_tag="VEW08_09065"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HYJ40927.1"
                     /translation="MKPIARSWWLSPGGVLLLIALVSGALAWWLIALPVLTLANVDGH
                     PQHFSMVFAHMATGTLMLFVGAANIYVGTTRRFFAHHKTLGYLYLAGGTVGASLALIL
                     ALRNPHGAGGQLPGGMQGTGDLGYALAALAIAWLVSAAMGYRAVRNRRHDTHRAWMIR
                     SYVLTWSFVLCRLVGKIPALADLGDGSAIIWLSWIVPLFACEVALQWQSGSARASAGT
                     "
     gene            complement(4109..>4455)
                     /locus_tag="VEW08_09070"
     CDS             complement(4109..>4455)
                     /locus_tag="VEW08_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40928.1"
                     /translation="VNHESVRRVERIVELNPANGRALSFGAGALYEDGQRERALEWAR
                     RAESLYPDDMGVIINGALLRARSGLSQEALDLLERVFNKGWGKKDWVENDPDYDSLRA
                     EPRFIAMMGKLK"
BASE COUNT          809 a         1433 c         1444 g          769 t
ORIGIN      
        1 tccatgagcc gcggttccat ccacgcggtt tcgatgccgt cctgctgcac ggtgttgacg
       61 cgtcgctcgt gcggcggaca cgacagcagc acctggcgca gccacggcgc gagcggaacg
      121 gtctggcagc tcatgcgtaa ccctggatga tcgagccgcg cgtaatcgag catgtcggaa
      181 atcaggtcgt cgatctgctg cacgtcggcg ttcacggctc gcaactgggc gcgcagcggt
      241 tcgtccactt caccttcgag tactgcggcc gcgaagcgca cgcgcgccag cggcgtgcgc
      301 atttcatgcg agagcgcggc ggtcatttcc ttctgcgact ggatgagctg gctcacgcgc
      361 gcggacatgt cgtcgagatt ggtggcgagg ctggtgagct gcgtggtgtg ccttgcggtg
      421 tcgagcggct cgcgatagtc cgtcgcgaat ttctgcgaag cgacggtcag cacacgcaga
      481 tcgcgaagca gcggccgcag ccacaggccg acgatcagca ggatggaagc gtagaacagc
      541 acgggcagga accgcacccc gaggctttcg cgcgctggct cgaatggtcc cagccggatg
      601 gcgcccttca gggcttccga atgccacaga aagtagatgc ggccgtcgtc atccgtaagt
      661 tcgcgcgggc cggactcgag tggcgcggcg cccgagatgt cggccgcctc gaggacgtcg
      721 agctcgagct tcagtttctc cgcgaggcgc gcggcgagcg cggcgcgttc gctgcctgga
      781 acggcggcga gttgcgattc catgagttga aatgtggata cgagccaggg atccgcggcg
      841 cgcacctgcc gcgactgcaa tgccgagaac ccgaggtcga gcaggacagc gaccagcagc
      901 aacccgccgg caagcaccac gtaggagcgg atgaacagcc cgcgatgacg ggcctgcagc
      961 cattccctca ttcccaggcg tcgggaacta acaagtatcc cttaccccac actgtcttga
     1021 tgcgcgtcgg cgccgcggcg tcgtcgccga gcttgcggcg caacttggac acgcggccat
     1081 cgatggagcg atcgaggccg tcgtagtcga tgccgcgcat ggtgcggaac acttcatcgc
     1141 ggctcaccag ctcaccggcg cgatgcgcca gcaggctgag caattcgaac tcctgggtgg
     1201 tgagcggcac gggctggctc tgcaaccaca cttcgcgcga ggtctcgctg atgcgcaagg
     1261 cgccgagcat caggtccggc ttgtgtgtgc cggtactcgg agtcgcgcgt tcagcgcggc
     1321 gcatgagtgc gcgcacgcgc gccagcagca ccatcggatc gacgggcttc atgacgtagt
     1381 cgtcggcgcc ggcctcgagg ccgatgacat ggtcgatgtc ggtgtcgcgc gccgtcagga
     1441 tgaggatggg cacgtcatgc acgcgccgca attcgcggca gatgctcagc ccgtccttgc
     1501 ccggcagcag cagatcgagc agcaccagcc gcggcttctc gcgcgcgaat ctttcgaccg
     1561 cgcgatcgcc gcgttcttcc accgcgacgc gcaggccgtt ctgctcgaga taggtggcgg
     1621 tcagctccgc caggcgggag tcgtcttcga tcagcaggat gtcgacgctt ggagtattca
     1681 taggacgtcc cacacatgcc gcgtgcgccg ctcgcgccgc cggtcatccg agcccacgcg
     1741 gagcacctcg attttcgcct cggcgacgcg ctcggtgcca gcgggcggcg agagccagaa
     1801 tacgaagtct tcgctgacca cgcgctcctg tgagtgttca ccggaatccg tgcgcggcca
     1861 gcaccgcaaa ggcgtctgcc gggcgctgtc gttcaaacaa tactcggccg cgagcgtgct
     1921 cttccagcgg acatcgaagt tgatcgtgca ggccacctcg tccttgtcga tgacacacag
     1981 caggggtttg acgctcagtc gcacgggcag cggatcggcg gcggcggcgg tcatggctac
     2041 ccccaggccc cctgcgatga agatggtgag gcgcgcgagc atcagaactg ccaggccatt
     2101 cccgcgaagt agccgagcac ctgcttgtcc tcgaccagcg ggctctgcgc cacgctgtgc
     2161 tgcaggcgtt cgtagttggc cgacaccgcg atacgaacgc tcttggtcag gtagtagcta
     2221 gtacgcaggc ccgcctccca gccaacgccg ccctcggctt cgtattcggg caacaggaac
     2281 gtggcctcat ccgcgtgcac gccccagtag tagtcgctga gcgccgcact cttgtaggag
     2341 acgcccacgt tgggcgcgat agaccatcgg ccgcgcaccc agcgaaagcc atagtcgacg
     2401 gacagctcgt agccatgatg cgtgccgctg acgtcgtgaa aggcgcgcag ggcggccatg
     2461 ccccattcac cgtccatcaa catttcgaag ccagcttcga cggcgtagtc gcgtttgggc
     2521 ggcttgaact tcaccggctg ctccaccaga ttgccggacc ccggatcgac ggccgggccg
     2581 actacaccgc cgcccaggta ggagaagttg atgtacttgg tgttggtctt gctgaagaac
     2641 gcccggtcac tgttcactcg cgcgaccagg ttcgtggtga agctcggccg gtcgaacagc
     2701 tcgaagccga gatcgaaatt gtcgaagaac cagcgctcgc cgaaccaggc gatgtcgagg
     2761 tcgacgatga cggggatgtc gtcagactgg accagcgggt tggtgcgttc cccgtaaccc
     2821 agggccactc cgaagcgcca attcttttca accggcagag ggcgaggggg ggtcgtggcc
     2881 ggggcaggcg tcgaggcggc ggggattggg ggcgcttcgg ttgcaggcgc atcttgcgcc
     2941 gcagccagct gcgcgcagga ccacaacgcc gccactgcca gcaggtgctg gaacctgaag
     3001 gttttcatga ggacattcta actatggagg cccgtgcaac cggagaaacc ggcgcgcaaa
     3061 gctgtgtgaa aaatgtgcaa aagtgtcgaa gcggccggcg acacaatttg acgaattccc
     3121 ccaaatgtcg cgacagttcc ctttcggccc ggaagtaggc tttttcccga aacatagggg
     3181 aatgagtcta cgtcatgcgc cgcccgatca actggttcgt gttttgcctg tcgctcgcgt
     3241 ttctgtttgc gaacgtttcg ctgagccgca aggcctctcc gcccgtggaa ctggccgcta
     3301 ttcccgtcac ggcggaactg gatttctcac acgtcatcct cgcggagacc acccctccac
     3361 tgttcggcta tatccgcgac gacggttcat tcatgccgct tgcccaacag tcgcccaagc
     3421 cgtctgccac ttcgaaggtt cccgcacgct tctgatcagg tgcccgcaga tgcccgggcg
     3481 ctgcccgact gccattgcaa cgcgacctcg caggcgaaaa gcggcacgat ccaggacagc
     3541 cagatgatgg cgctgccatc accgagatcg gcgagcgccg gaattttgcc gacgaggcga
     3601 cacagaacga aagaccacgt gagcacgtag ctgcggatca tccacgcccg gtgcgtgtcg
     3661 tgacgtcgat tgcgtacggc gcgataaccc atggctgccg acaccaacca ggcgatcgcg
     3721 agtgcggcga gcgcgtagcc gagatctccg gtgccctgca ttcctcccgg gagctggccg
     3781 ccggctccgt gggggttacg caatgccagt atcagcgcca gcgaggcgcc aaccgttccg
     3841 ccggcgagat atagatagcc caaggttttg tggtgcgcga agaagcgccg cgtcgtgccg
     3901 acgtaaatat tcgcggcgcc gacgaacaac atgagagtgc cagtcgccat gtgtgcgaac
     3961 accatggaga agtgttgcgg atgtccatcc acgttggcca gggtgagcac cggcaacgcg
     4021 atcagccacc acgcgagggc gccgctcacc agggcaatga ggagcaggac cccgccgggc
     4081 gaaagccacc acgaacgcgc gattggcttc atttcagctt gcccatcatg gcaatgaacc
     4141 gcggctcggc gcgcaggctg tcgtaatccg gatcgttctc aacccagtcc tttttgcccc
     4201 agcccttgtt gaagacgcgt tcgagcagat cgagcgcttc ctgtgagaga ccggatcggg
     4261 cgcgcagcag tgcgccgttg atgatcacgc ccatgtcgtc ggggtagagc gactccgcgc
     4321 ggcgcgccca ttccagcgct cgctcgcgct ggccgtcttc gtacaaggcc ccggcgccaa
     4381 acgacagtgc ccggccgttg gcggggttga gctcgactat gcgttcgacc ctgcgcacag
     4441 attcgtgatt gaccg
//
LOCUS       DASQJS010000223         5662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3899989, whole genome shotgun sequence.
ACCESSION   DASQJS010000223 DASQJS010000000
VERSION     DASQJS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3899989"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..820)
                     /locus_tag="VEW08_09075"
     CDS             complement(149..820)
                     /locus_tag="VEW08_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYJ40929.1"
                     /translation="MAIDVYWGSGSPYSWRVLLALEHKGLEYRSHLLSLAMQEHKTPH
                     MMAMNPRGRVPVLKDGDYVVFESLAVLYYLDRKYPAKPLFGDSAEEGAVIMRVCCEYQ
                     AYIEPHVMRIVRALLNRIGDPQSDESTYAMHMIAGEARTIEGRLSKGTWIVGENPGAS
                     DYMIYPGIKLLLRALALPEARELSSRFLPVEANYPALSRWLQRVESLPGYERTFPPHW
                     RDANS"
     gene            847..1701
                     /locus_tag="VEW08_09080"
     CDS             847..1701
                     /locus_tag="VEW08_09080"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HYJ40930.1"
                     /translation="MHHFTTLIDANTLSEHLSREDLTIFDCRFDLGNVSWGEVEYSRA
                     HLPGALYLHLDRDLSSPVTASSGRHPLPDPERFAARLAALGVSSDCQIVAYDQGNSAY
                     GARLWWLARWIGLRNVAVLDGGIAAWRAAGLPLETALRTPQPRPLTLSLAADAWVSSE
                     TVDELRQRPGNLLIDARGAERFAGRNETIDPVPGHVPGARNRPFLDNLGADGRFLPAE
                     VLRQRFGTLLGSVEPSSLIAMCGSGVTACHNLLALEHAGLTGARLYAGSWSEWIRDPR
                     RPIATGPY"
     gene            1779..3749
                     /gene="speA"
                     /locus_tag="VEW08_09085"
     CDS             1779..3749
                     /gene="speA"
                     /locus_tag="VEW08_09085"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HYJ40931.1"
                     /translation="MSAEPNSPSASEAITRRSERPIAWSVDDSAELYQVNAWGKGYFC
                     VNPSGHLVVRPDMNAEREIDLFDVVQGLKERELTTPVVVRFSDILAHRLKHLHDAFAQ
                     AIAENDYQNRYTAVFPIKVNQQRLVVEEVYRYGQPFGFGLEVGSKPELLSVMTMTADS
                     PERLIVCNGFKDDSYLEAVIIATKLGRTIIPVVENFSELPAILKYAEKYNVRPRIGVR
                     VKLASEGSGKWRDSAGDKSKFGLFITEILEAVEILREKGMLDCLQLVHCHPGSQLQDI
                     RRVKDAINELAHVYAELKLLGAGLQYIDVGGGLGVDYDGSGTNFSSSMNYTLNEYAND
                     VVYRIANVCNTRKIAHPMIVSESGRAIAAHHSVLIFNTLGTSALDQFRVTGKEDQQHG
                     RELPQPVRDLLDAFRTVTERRVVECYHDAQTARDQVLQMFNLGLLSLEQRGLAERLYW
                     ATCAKVRDLTRKLDEVPEELEELESILSDIYFCNFSVFQSLPDSWAIDQLFPIMPIHR
                     LNERPTRKGVLADITCDSDGKIDRFVSQRDVKRTLELHSITAQDEYYLGVFLVGAYQE
                     TLGDLHNLFGDTHVVHVRFHDDGGWWIEEIVKGDTANKVLEYMEYDVADLHPALARDC
                     ERAVREGRMSVAESQGIKRFYEGELNGYAYLE"
     gene            4036..4320
                     /locus_tag="VEW08_09090"
     CDS             4036..4320
                     /locus_tag="VEW08_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HYJ40932.1"
                     /translation="MTTIYVGNLPFSATEQDIKVLFERHGKVDSVKLINDRETGKPRG
                     FGFVEMGSGEAQAAIQGLNGYQMNGRPLRVNEAQERPPRPRQGGGGPFRR"
     gene            4467..5327
                     /locus_tag="VEW08_09095"
     CDS             4467..5327
                     /locus_tag="VEW08_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40933.1"
                     /translation="MPRSALALIAACLLAACASAPVEVAAPPASPAPQPASRDEATAA
                     AIELVLAGEHRSTGNRARDAYRHPLDTLLFFGIKPDMTVVEVWPGAHGWYTEVLAPLL
                     AERGRLYVATMPPKPGNAYVTANFRSFTDKLAARPDLYGRVGVTTLAPGNFAVAPPGS
                     ADLVVTFRNIHNWMNLGMAPDAFAEMFRALKPGGILGVVEHRGPAKPQDPRAVSGYVN
                     EDYAIAMIEAAGFELVARSPINDNPKDTKDYPQGVWTLPPDYRLGNRDRAKYEAIGES
                     DRFTLKFRKP"
     gene            complement(5381..>5662)
                     /locus_tag="VEW08_09100"
     CDS             complement(5381..>5662)
                     /locus_tag="VEW08_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYJ40934.1"
                     /translation="AELIEEERRVLYVAMTRARNELTCIVPLRFQITSQPKTSDGHVY
                     GGRSRFLTEKVIKCFDEQTFQGSNVDVKIDDAPADSGTIDVASRLKQMW"
BASE COUNT         1092 a         1838 c         1713 g         1019 t
ORIGIN      
        1 agcgtgccct gaccgatgct gccgaatccg atgaagacga tacgcccggg gaactcgata
       61 tgaatcttgt ggttcgacat tatttacccg tccaaaatca gggcgtgagg gtatcaacca
      121 tcatccggcg ttgccaccgg atacggtgtc acgaattggc gtcacgccag tgcggtggaa
      181 acgtacgctc gtaacccggc agcgattcga ctcgctgcag ccagcggctg agcgccggat
      241 agttcgcttc cacgggcaga aaacgcgacg acaactcgcg cgcttcgggc agcgccagtg
      301 cgcgcaacag cagcttgatg cccgggtaga tcatgtaatc cgacgcgccc ggattttcgc
      361 ccacgatcca cgtccccttc gacagacggc cttcgatggt gcgcgcttca ccggcgatca
      421 tgtgcatggc gtaggtgctc tcatcggact gcggatcgcc gatgcgattg agcagcgcgc
      481 gcacgatacg catcacgtgc ggctcgatgt acgcctggta ctcgcagcac acgcgcatga
      541 tgaccgcgcc ctcttccgca gaatcgccaa acaacggttt cgccggatac ttgcgatcga
      601 ggtagtagag aaccgccagc gactcgaaca cgacgtagtc gccatccttg agcacgggta
      661 cgcggccgcg cgggttcatc gccatcatgt gaggagtctt gtgctcctgc atcgccagcg
      721 acaacaggtg gctgcggtac tcgagtccct tgtgttcgag cgcgagcaac acgcgccacg
      781 aatacggact gccgctaccc caataaacgt cgatggccat gagtgttccg gattgtaaaa
      841 tggggcatgc accatttcac cacgctcatc gacgcgaaca cactctccga acacctttcg
      901 cgtgaggacc tgacgatatt cgattgccgc ttcgacctcg gcaatgtgtc ctggggtgag
      961 gtcgaatact cccgggcgca tcttcccggc gcgctctacc tgcatctcga tcgcgacctc
     1021 tccagcccgg taaccgcgag cagtggccgt catccactgc cggatcccga acgctttgcc
     1081 gcccggctcg ccgcgctcgg cgtgagctcc gactgccaga tcgtcgcgta tgaccagggc
     1141 aattcggcgt atggagcgcg cctgtggtgg ctggcgcgct ggatcggctt gcgcaatgtc
     1201 gcggtgctcg acggcggaat cgccgcctgg cgcgcagccg gcctgccgct cgaaactgcg
     1261 ttgcgcacac cgcaaccgcg cccgctcacc ctgtcgctcg cggcggatgc ctgggtgagc
     1321 agcgaaaccg tcgatgaact gcggcagcgt cccggcaacc tgctgatcga cgcacgagga
     1381 gcagagcgat ttgcgggacg aaatgaaacc atcgatccgg tacccggcca cgtgccaggc
     1441 gccagaaacc gtccattcct cgacaatctc ggtgccgatg gtcgattcct gcccgccgag
     1501 gtgctgcggc agcgcttcgg caccctgctg ggctcagtgg agccttcttc gctgatcgcc
     1561 atgtgcggct cgggagtcac cgcctgccac aatctgctcg ctctcgagca cgccggactc
     1621 accggcgcgc gtctttatgc cggttcctgg agcgagtgga tccgcgatcc gcgccgtccg
     1681 atcgccaccg gtccgtacta gaggccgaag taaatgccga agtaagggct gcatctggcg
     1741 tttacttgca aaccctattt gctatcgtgc gcgccaccgt gtcagctgaa cccaattccc
     1801 cctccgcgag cgaagcaatc acgcgccgct ccgaacgacc cattgcctgg tcggtcgacg
     1861 attccgcaga gctctaccag gtcaatgcct ggggcaaggg ctatttctgc gtgaatccgt
     1921 ccggccacct cgtcgtgcgc cccgacatga acgccgagcg cgagatcgac ctgttcgatg
     1981 tcgtgcaggg cctcaaggag cgggagctca ccacacctgt ggtggtccgc ttctccgaca
     2041 tccttgcgca ccggctcaaa catctgcatg acgctttcgc acaggcgatc gccgagaacg
     2101 attaccagaa tcgctacacg gcggtgttcc ccatcaaggt gaaccagcag cgcctggtgg
     2161 tcgaagaggt gtatcgctac ggccaaccct ttggcttcgg actcgaggtg ggcagcaagc
     2221 ccgagcttct gtcggtcatg accatgacgg cggattcacc ggagaggctc atcgtctgca
     2281 acggcttcaa ggacgacagc tatctcgaag cggtgatcat cgcgaccaag ctcggccgaa
     2341 ccatcattcc cgtggtcgag aacttcagcg aactgccggc aatcctgaag tacgccgaga
     2401 agtacaacgt gcggccgcgc atcggtgtgc gcgtgaaact ggcgagcgaa ggctctggca
     2461 agtggcgcga ctcggccggc gacaagtcca agttcggcct gttcatcacg gaaattctcg
     2521 aggctgtcga gatcctgcgc gagaaaggca tgctcgactg cctgcagctc gtgcactgcc
     2581 atccgggcag ccagctccag gacatccgcc gcgtgaaaga cgccatcaac gagctcgcgc
     2641 acgtctacgc ggagctcaag ttgttaggcg ccggattgca gtacatcgac gtgggcggcg
     2701 gcctgggcgt cgactacgac ggcagtggca ccaacttctc ttcgtcgatg aactacacgc
     2761 tcaacgaata cgccaacgac gtggtgtatc gcatcgccaa cgtgtgcaac acgcgcaaga
     2821 ttgcgcaccc gatgatcgtc agcgaatccg gccgcgccat cgccgcgcac cacagcgtgc
     2881 tcatcttcaa cacgctcggc acttccgccc tcgaccagtt ccgcgtcacg ggcaaggaag
     2941 atcagcaaca cgggcgcgag ctgccgcaac cggtacgcga cctgctggac gcgtttcgca
     3001 ccgtcaccga gcgcagagtg gtcgagtgtt atcacgacgc gcagaccgcg cgcgaccagg
     3061 tgctgcagat gttcaacctc gggctgctgt cactcgaaca gcgcggcctg gcagaacgcc
     3121 tgtactgggc cacgtgtgcc aaggtgcgcg atctcacgcg caagctggac gaagtgcccg
     3181 aggagctcga agaactcgag tccatcctct cggacatcta cttctgcaat ttctcggtct
     3241 tccagtcgct gcccgacagc tgggccatcg accagctgtt ccccatcatg ccgatacacc
     3301 ggctcaacga gcgcccgaca cgcaagggcg tgctggccga catcacctgc gactccgacg
     3361 gcaagatcga tcgtttcgtg agccagcgcg acgtgaagcg cacgctcgag ctgcattcca
     3421 tcaccgctca ggacgagtac tacctcggcg ttttcctggt gggcgcctac caggaaacgc
     3481 tcggcgacct gcacaacctg ttcggcgaca cccacgtcgt gcacgttcgt ttccacgacg
     3541 acggcggctg gtggatagag gaaatcgtca agggcgacac ggcaaacaag gtgctggagt
     3601 acatggagta cgacgtcgcc gacctgcacc ccgcgctggc gcgcgactgc gaacgggcgg
     3661 tgcgcgaagg acggatgtcg gtagccgagt cgcagggcat caagcggttc tacgaaggcg
     3721 agctcaacgg ctacgcctat ctagagtaac gccggcggca tagacataag cgtgtccttg
     3781 tcggacggga ccggtaccaa aaccgcgctc cggcggccga aaactggcgg aaacgcctga
     3841 aaatgcgccg gtcgccgaac ccgcccggct acccacccga cccgttgttt acagcttctc
     3901 aaaccgagga gtagcatcat ccgcgttcgc cttgaaccac ggtgcaggcc gcctcgacct
     3961 gttttttatt gtccgcggta catgcagggg accgcatttc ctgccgctgt ccgctttttg
     4021 agcgagtagt ccgggatgac gacgatctac gtgggcaatc tgcccttctc cgcaaccgag
     4081 caagacatca aggtgctctt cgagcgccat ggcaaggttg attccgtaaa gctgatcaac
     4141 gaccgcgaga cgggcaagcc ccgtggtttt ggtttcgttg agatgggatc cggcgaagca
     4201 caagccgcga tccagggtct caacggatac cagatgaacg gccgtccgct tcgtgtgaac
     4261 gaagcgcagg aacgcccgcc gcgccctcgc cagggtggtg gcggaccgtt ccgccgctga
     4321 ttggggtcag gcaccgtttc cagaaaacgg tgccagagtc gatcagctag ataggacggt
     4381 gcggctctgg caccgttttc aggaaacggt gcctgacccc aaccccgccg tgcgcggggt
     4441 tcttttttct ttgcctggag aatgcgatgc cccggagcgc cctcgcgctg atcgcggcct
     4501 gcctgctggc cgcctgcgct tcggcgcccg tcgaagtcgc ggctccgccg gcgtcgccgg
     4561 ctccgcagcc cgcatcgcgc gacgaagcga ccgccgcggc cattgaactc gtgcttgccg
     4621 gcgaacatcg cagcaccggg aaccgcgcgc gcgacgcata ccgccatcca ctcgacaccc
     4681 tgctgttctt cggcatcaag cccgacatga ccgtggtcga ggtgtggccc ggcgcccacg
     4741 gctggtacac cgaggtgctc gcgccgctgc tggcggaacg cggccggctg tatgtggcca
     4801 cgatgccgcc gaagcccggc aacgcctatg tcaccgcgaa cttcaggagt ttcaccgaca
     4861 agctcgccgc gcgcccggac ctgtacggcc gcgtcggagt caccacgctt gcgccaggca
     4921 acttcgcggt tgccccgccg ggcagcgccg acctggtggt gacgttccgc aatatccaca
     4981 actggatgaa cctcggcatg gcgccggacg cgtttgcgga gatgttcagg gcgttgaagc
     5041 cgggcggcat cctgggcgtg gtcgaacatc gtggaccggc gaaacctcag gacccacgag
     5101 ccgtcagcgg ctatgtcaac gaggattacg ccatcgcgat gatcgaagcg gcgggcttcg
     5161 agctggtagc gcgttcaccg atcaacgaca acccgaagga cacgaaggat tacccgcagg
     5221 gcgtgtggac gcttccgcct gactatcggc tgggtaacag ggatcgagcg aaatacgaag
     5281 cgatcggcga aagcgaccgc tttacattga agttcaggaa gccgtgagaa aaggggacag
     5341 atctattttt cgctgcggaa aatagatctg tccccttttc tcaccacatc tgcttcaggc
     5401 gcgatgccac gtcgatggtg cccgaatccg ccggtgcatc gtcgatcttc acgtcgacgt
     5461 tcgaaccctg gaaggtttgc tcgtcgaagc acttgatgac cttctcggtg aggaagcggc
     5521 tgcggccacc atagacatgg ccgtccgaag tcttcggctg gctggtgatc tggaagcgca
     5581 acggcacgat gcaggtgagc tcgttgcgcg cacgggtcat ggccacatac agcacccggc
     5641 gctcttcttc gatgagctcg gc
//
LOCUS       DASQJS010000224        15848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_758004, whole genome shotgun sequence.
ACCESSION   DASQJS010000224 DASQJS010000000
VERSION     DASQJS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15848
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758004"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /gene="nirD"
                     /locus_tag="VEW08_09105"
     CDS             complement(<1..993)
                     /gene="nirD"
                     /locus_tag="VEW08_09105"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HYJ40935.1"
                     /translation="MSNARWHDVCGLEDLIEGAGVAALIGGRQLALFQVHGRVYALDN
                     FDPASRANVLSRGLTGNLQGERVVASPIYKNHYSLTSGRCMEDPTFNLTAYPTRVTEG
                     RVFVESPDRVRRTRLVVAGNGMAGMRTVEELLKLGIADRFSITVFGAEPRGNYNRILL
                     SPVLSGEQQADDIMLHRPNWYTRRGITLHSGDPIVEIDRKRRTVRSRNGTVADYDRLI
                     IATGSDPIVLPLPGKDLPGVVTFRDLDDVNKMLERSGDGRRAVVIGGGLLGLEAAHGL
                     ALRGMHVTVVHLLDTLMERQLDASAGALLKAALEKRGIEFRMRAKTAAILGGERV"
     gene            complement(990..3548)
                     /gene="nirB"
                     /locus_tag="VEW08_09110"
     CDS             complement(990..3548)
                     /gene="nirB"
                     /locus_tag="VEW08_09110"
                     /inference="COORDINATES: protein motif:HMM:NF011565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HYJ40936.1"
                     /translation="MSKRIVVVGHGMVGQRFLETLTKDGQSDFAITMLAEEPRLAYDR
                     VNLSKFFSGSSVEDLTLTTREFFDKNGIELYSGDPVAGIDRVNKRVTTKSGAEYEYDI
                     LILATGSSPFVPPIPGRERPGVFVYRTIEDLEAISEAGKNSTRGVVIGGGLLGLEAAK
                     ALKDLGLVTNVVEFAPRLMAVQVDEGGGGLLRSRIEALGVAVHTGKNTTRIDDGESAR
                     HRMHFADGGSLETDVVVISAGIRPRDELARACGLEVAPRGGISINGDCRTSDESIYAI
                     GECAAYEGRVFGLVAPGYQMARIAAARVLGTEGEAFAGADMSTKLKLLGVEVGSIGDA
                     HAATPGARVYSFFDQRKEVYKKLVVDAEGKKLIGAVLVGDTSDYSMWHQTVANAMALP
                     ENPETMLFPAAVASAGAPPTNALAALPATAQICSCNNVSKGGICSAIEAGCTTVGALK
                     TKTKAGTTCGGCTPLVTQILNAELKKRGVNVNTSMCEHFPYTRQQLFHLVRIGELKTF
                     EDLLARHGRGLGCDICKPAVASIMASCWNEFVLKKRHAALQDTNDYFLANMQKDGTYS
                     VVPRVPGGEITPEKLIVIGQVAKKFGLYTKITGGQRIDLFGAQVHQLPLIWKELIDAG
                     MESGHAYGKALRTVKSCVGSTWCRYGVQDSVGLAIDLENRYKGLRAPHKIKFAVSGCT
                     RECAEAQSKDVGVIATEGGWNLYVSGNGGMKPRHGDLLARDLDTPTLIKFIDRYLMFY
                     IRTGDRLQRTSTWLENLEGGIDYVRKVVCEDSLGIAAELEADMARVIGNYACEWKVAI
                     EDPATLKRFRHFVNSNENDSNVVFVEERGQIRPASAEERVIAKELAKADEGVAA"
     gene            complement(3708..4502)
                     /locus_tag="VEW08_09115"
     CDS             complement(3708..4502)
                     /locus_tag="VEW08_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40937.1"
                     /translation="MRNRVLAVFTTWPFLLALAVLLVNDWLLKQAYPGLVTGKLSDFA
                     GIAVVSMPLFAAFPRHARAIYLAIFGAFLWWKSPASGAFIAFMNDVQPMKIGRTVDYW
                     DLMALAIMPACGKFAVSQPCSFVNPASLRRWVLPPVLAATLFGVVATSYPRHSNDFNI
                     RAVESSAPIPRDQIVAAISQVAKARGLKPQEPNPPHWAGAFMGRGIFLTYTFLGDNEI
                     GVGINIKSGMFGDSEMRRAEKLRAEIKKTLSLRFKGLEFVEPPPKP"
     gene            complement(4515..4997)
                     /locus_tag="VEW08_09120"
     CDS             complement(4515..4997)
                     /locus_tag="VEW08_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ40938.1"
                     /translation="MNAPDHSIVPVRDESDIALAKELFVEYAASISIDLEYQGFSAEL
                     AGLPGKYSPPTGDLLIARVNGEAVGCVALRALDRATIEMKRLYVRPVARGLGLGKRLG
                     EAAISCAREYGYSELRLDTLATMVSAQALYRSLGFVEIPPYGAAHVPGTVFFGLSLTA
                     "
     gene            complement(4994..5278)
                     /locus_tag="VEW08_09125"
     CDS             complement(4994..5278)
                     /locus_tag="VEW08_09125"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HYJ40939.1"
                     /translation="MLFMVIERFRDNDMVPIYQRVRDSGRAIPSGLKYIDSWVEPNFS
                     RCFQLMECDDLRLLQAWVLQWRGAGVTFEIVPVVSSAQTREVVAPHLSDT"
     gene            complement(5288..5695)
                     /locus_tag="VEW08_09130"
     CDS             complement(5288..5695)
                     /locus_tag="VEW08_09130"
                     /inference="COORDINATES: protein motif:HMM:NF020457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HYJ40940.1"
                     /translation="MSRKSPDPTDPYHAHVYYNEDTWDVAQRLHQELSDMLVSGTLAN
                     LVMVGRMYETGVGPHPMPQFEIQFLASAVPDITRILEATGLNALIHPLTDDDLADHTT
                     LAVWLGEPIPLDESVLDPPGQNQGIARFGKVEF"
     gene            complement(5685..6110)
                     /locus_tag="VEW08_09135"
     CDS             complement(5685..6110)
                     /locus_tag="VEW08_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYJ40941.1"
                     /translation="MNTRSAFYAPLLLRVTLGTLFVAHLYWKFAVLPGGFDQWWSNFA
                     SNGYPWFVPWYVLSAEIAGALLLIPGIRVRWVCLYAVPMMLGAAQFWATRKGFWFVGG
                     GAELPVVWCIMLLLQAMLGDGPYALSPSPDRTRELRRES"
     gene            complement(6165..10049)
                     /gene="hrpA"
                     /locus_tag="VEW08_09140"
     CDS             complement(6165..10049)
                     /gene="hrpA"
                     /locus_tag="VEW08_09140"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HYJ40942.1"
                     /translation="MNPPNPAPEPSIDTCLRRDQHRLRRERDRLHGLRLRGNDVVAAE
                     AALAQKINVSTAARAARAASVPAVSYPEELPISANRDEIRRAIEKHPVVILCGETGSG
                     KTTQLPKICLEAGRGVAGVIGHTQPRRIAARAVGARIAEELKTPLGQLVGYKLRFQDH
                     SRPEGLIKLMTDGILLAETQGDRFLDAYDTIIIDEAHERSLNIDFLLGYLKWLLPKRP
                     DLKLVITSATIDPERFSKHFGDAPIINVSGRSYPVEVRYRPVELDEEDDETASGEQDA
                     ILSAVDELWSEQAGDILVFLSGEREIRETAESLRKHHPQGCEVLPLYSRLSAEEQQRV
                     FRPSGRRRVVLATNVAETSLTVPGIRAVIDTGVARISRYSHRTRLQRLPIEKISQASA
                     SQRSGRCGRVGPGVAIRLYSEEDFGARPAFTEPEIQRTNLAAVILQMHALKLGDIEAF
                     PFVEPPDGRFVRDGQRTLRELGALSEEGQLTDTGRRLAKLPLDPRLGRILLAGAEEHC
                     LEEVAIIAAALSVPDPRDRPADKQTQADQKHAPLRDEQSDFLSLLKLWRAFEEQRAHL
                     SRARLRGWCKENFLSYLRLTEWHDVHGQVMEVVKGELALKLNAQPGQYASIHRALLAG
                     LLSQVANRREQAEYLGANGTKLFIHPGSGQFKAKPAWIVSAEQVETTKVYARNVARVE
                     PAWIEQAGAHLVKRHHYEPHWERRASRAAVYERVTLFGLLLSSGRSVPYEQIDPKAAR
                     ELFIKHALVHMEYDSRAPFLAHNLKLLKDTEYLQQKGRRVDLLGDETQLYAFFDARVP
                     EGISTGAAFERWRREAEAKNPKILWLTEQDISPGEAELDAEQFPDHLSAGPLVIQLRY
                     RFEPGHEDDGVSALIPLHVLNQLPEEAFEWLVPGLLDEKIEALVRSLPKNLRVHFVPV
                     PDAVARVLPLLERGIGSLYAQLADALLRTGGVPVPRDAFREDLLSPHLRVNFVLLDDA
                     DKVIARSRSLTSLRGKHAGASQQHYAKQSQLTTGAKSWVFGDLPDKQDVQAARAQTGY
                     PALVDEGESVGLRVFATPAEARISHERGCARLIRIVMARDFKSYRRDLPVNVQAEIVY
                     RGLAQHPLLNADLVAGRDLREDLLDRIVMTVFLEGHEPLRSGAGFDARIAELRGGLGL
                     PAQEISRIVQGSLEQLGRIQAALPKAAASAAADIRSQLAWLAPAGFLLTTPRERLKEF
                     PRYLKAIEQRLEKLGRDPRRDAQLAAEIAPLETRYRERVKAERGQRPPGDDEFRWMLE
                     EFRVSLFAQQLGTRGTVSARRLGDAWVVRERA"
     gene            10145..12361
                     /locus_tag="VEW08_09145"
     CDS             10145..12361
                     /locus_tag="VEW08_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ40943.1"
                     /translation="MAAYSVAGFHVITGVCVLAAVRHMSTGLWRPIDKTNLLFSLMCC
                     FAAGMALTQAQLYQAWSIEEHRSVLKWNITFALAFFLVLPWFATAVAGRQPVRWLIAS
                     TIVFIVLMVLNLIQPFGVQLAGIDRIETKLMPWGEEYASPVGPIAPGFWFAISAVLIA
                     ICDAVIRFWSGWRRQKSGSSLLLVLSTTAYLVAGIEGILVRAQMIDFVHLGPFGFFLM
                     VIAMSLTLSFRSRQTLMVSEQRFRALVEQSPFGIQVLASDGSTVQVNPAWERLWGKST
                     PESPRVADERLLPTLERAFLGHKGETNARPAEGGKWIRSFAYPILDSFGVVRNVIVMH
                     EDITEEKRAEDSARAVTAELSQVKHAVLQQERLRALGQMAGGIAHDINNAISPAAMYI
                     EALLEQDKTLNSRARERLSIVQQAIASVVQTVSRLREFCRPREAQATREDVDINEVVK
                     QSVVLTQARWKTMAQGKGIAIRMVTNLADGLPTIKGSASEIRDAIVNLIFNAVDAMPQ
                     GGTITLQTRALGNGASIEVSDTGTGMDEQTRRRCMEPFFSTKGEHGSGLGLAMVYGML
                     KRHGGEIEIVSAPGSGTTMRLLLSTDSTRMERPPATSPSHDTASGRTTGARILLADDD
                     PLLLESLRATLEGDGHHVLTADDGQSAIDAFEAADKAGAPFAAVITDFVMPIVDGRQL
                     SSVINQVRPGTPVIMLTAYGNRIRAQDDTLPYVDHILAKPANLAELRNTLERLLNR"
     gene            complement(12358..13425)
                     /locus_tag="VEW08_09150"
     CDS             complement(12358..13425)
                     /locus_tag="VEW08_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HYJ40944.1"
                     /translation="MKKQCASLLAVMLGVLLSSAAWAEDLDAWTAAKLMGTGVNIGNT
                     LENTTTWETGWGNPPITKKFVESLAKLGFKTVRVPVAWDTYAHNGRIDARKLARVGEV
                     VDWITAAGMFCVVNIHWDGGWIDSSAKEKFPKTFATFSADAESKFVSYWRQIASYFAD
                     RNERLLFEALNEETNFEGTGSTKKAYATLTRVNQLFIDTVRRTGGNNAKRLLIVTGYS
                     TDITRTTSSEYVLPTDSVPHRLLISVHYYTPWQFAGMTKDESWRKMQLTWGSAADVAE
                     LNRLFDAMQEFCKRNDLPAFIGEFGVTDKKEMASRVRWMSAVAEAALSRQMVPVLWDT
                     GGDISRKPPYAPSAALSEVLR"
     gene            complement(13448..13855)
                     /locus_tag="VEW08_09155"
     CDS             complement(13448..13855)
                     /locus_tag="VEW08_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40945.1"
                     /translation="MYAIKVEIRNLKEKAFTFNAQKTMYGGKRIAKGDVIFIIASENE
                     GGPGLIARGVVTAAKATPRKPGIERQTPRVSIAVRRSALVKKRLGRSELKPFSNWKDG
                     RPETELNFKFYRQATNKIVGLSADAAAFLQRFF"
     gene            complement(13886..14782)
                     /locus_tag="VEW08_09160"
     CDS             complement(13886..14782)
                     /locus_tag="VEW08_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYJ40946.1"
                     /translation="MNDPLAEVITLLQPRAVFSKGISGAGRWGVRYSDFGHPSFCVVL
                     EGGCLLAVDKQKPLTLEAGDFVLLPATPGFVMSSYEPATPVRIDSKKAAGSLGEVRHG
                     RKGGRPDVRLLGGYFVFGSPDAALLVSLLPSLVHVRGVERLSTLVRLVDEESSGQRSG
                     RELVLSRLVEVLLVEALRATSGEDAPPGLLRGLADARVAHAMRVIHANPERSWTVAQL
                     ATKAALSRSTFFERFTRAVGQPPMEYLLAWRMAVAKDLLRRQDLALTEVAERVGYGSA
                     STFSTAFSRYVGQSPGRFARAS"
     gene            14877..15629
                     /locus_tag="VEW08_09165"
     CDS             14877..15629
                     /locus_tag="VEW08_09165"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ40947.1"
                     /translation="MKTVLITGCSSGYGLETARHFLSHGWKVIATMRTPRANILPASE
                     NLRVIALDVTRAESIAAALEASGPIDVLVNNAGIGLFGAFEATPMATTREVFETNTFG
                     AMAMTQAVLPQFRARRSGLIVNVTSSATLAPMPMVAVYTASKMAIEGFTASLAFELES
                     FNVRVKLVEPGYGPTTGFSSNSGSRMQGLIPEAYAPFAQNVFASFTQPGAVTKESDVA
                     EVVWQAANDASTRLRYAAGADAEALAKSARHV"
     gene            complement(15626..>15848)
                     /locus_tag="VEW08_09170"
     CDS             complement(15626..>15848)
                     /locus_tag="VEW08_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40948.1"
                     /translation="YVVFAGAYNEPDLLHLVELDRDEKATLVDTFRLVDINENPVSIE
                     RIVGRGKKLYALAGETVHVIDADDVIALS"
BASE COUNT         2879 a         5063 c         4945 g         2961 t
ORIGIN      
        1 cacgcgctcg ccgccgagga tggctgcggt tttggcgcgc atgcggaact cgatgccgcg
       61 tttctccagc gccgccttga gcagtgcacc cgccgacgcg tcgagctgac gctccatcag
      121 cgtgtcgagc aggtggacca cggtgacatg catgccgcgc agggcgagcc catgagccgc
      181 ttccaggcct aacaatccac caccgatgac cacggcgcgt cggccgtcgc cggagcgctc
      241 cagcatcttg ttgacgtcgt cgagatcgcg gaacgtgacc acgcccggca aatccttgcc
      301 cggcagcggc agcacgatgg gatcggatcc ggtggcgatg atcagccggt cgtagtccgc
      361 gaccgtgcca ttcctcgagc gaacggtccg ccgcttgcgg tcgatctcca cgatgggatc
      421 gccggaatgc agcgtgatgc cgcggcgcgt ataccaattg ggccggtgca gcatgatgtc
      481 gtcggcctgc tgctcaccgg aaagcaccgg cgacagcagg atgcggttgt agttgccgcg
      541 cggctcggcg ccgaacaccg tgatggaaaa gcggtcggca atgccgagct tgagcagctc
      601 ctcgacggtg cgcatgccgg ccatgccatt gcccgccacc accagccgcg tgcgccgcac
      661 gcggtccggc gattcgacga acacgcggcc ttcggtcacc cgcgtcgggt acgcggtgag
      721 gttgaaggtg ggatcttcca tgcatcgccc gctggtcagc gagtaatggt tcttgtagat
      781 aggcgacgcc accacgcgct caccctgcag gttgccggtc aggccgcgcg acagcacgtt
      841 ggcgcggctc gccgggtcga agttgtcgag cgcgtagacg cgcccatgta cctggaagag
      901 cgcgagctgc cgcccgccga tgagcgcggc cacgccggcg ccctcgatga gatcctcgag
      961 cccgcacacg tcatgccagc gtgcgttgct catgcagcca ccccttcatc agccttggcc
     1021 agctccttcg cgatcactcg ttcttcagcg ctggcaggac ggatctggcc ccgttcctcg
     1081 acgaacacca cgttggagtc gttttcgttc gagttcacga agtggcggaa gcgcttgagc
     1141 gtcgccggat cttcgatggc tactttccac tcgcaggcgt aattgccgat gacgcgcgcc
     1201 atgtcggcct cgagctccgc cgcgatgccg agcgaatcct cgcaaaccac cttgcgcaca
     1261 tagtcgatac cgccttcgag gttctcgagc caggtcgagg tgcgctgcag ccggtcgccc
     1321 gtgcggatgt agaacatcag gtagcggtcg atgaacttga tgagtgtcgg tgtatccagg
     1381 tcacgcgcca gcagatctcc gtggcgtggc ttcatgccgc cattgccgga gacatagagg
     1441 ttccagccac cttcggtggc gatgacgccc acgtccttgc tctgcgcctc cgcgcactcg
     1501 cgcgtgcatc cggacaccgc aaacttgatc ttgtgaggcg cgcgcaggcc cttgtagcga
     1561 ttctcgagat cgatcgcgag accgacgctg tcctggacgc cgtagcgaca ccacgtggaa
     1621 cccacgcaac tcttcaccgt gcgcagtgcc ttgccgtagg cgtggcccga ttccatgccg
     1681 gcatcgatga gttctttcca gatcagcgga agctggtgca cctgcgcgcc gaacaggtcg
     1741 atgcgctggc cgccggtgat cttggtgtac agcccgaact tcttcgccac ctggccgatg
     1801 acgatgagtt tctcgggtgt gatctcccca cccggcaccc tggggactac cgagtaagtg
     1861 ccgtccttct gcatgttggc caggaaatag tcgttggtgt cctgaagcgc cgcgtggcgc
     1921 ttcttgagta cgaactcgtt ccagcaggag gccatgatgg acgccaccgc cggtttgcag
     1981 atgtcgcagc caagtccgcg cccatggcgc gcgagcaggt cttcgaaggt cttgagctcg
     2041 ccgatacgta cgagatgaaa caactgctgc cgcgtgtacg gaaagtgttc acacatcgag
     2101 gtgtttacgt ttacaccgcg cttcttgagc tcggcgttga gtatctgcgt gaccaatggc
     2161 gtacaaccgc cgcaggtcgt gcccgctttc gtcttggtct tgagggcacc gaccgtcgtg
     2221 catcccgcct cgatggccga gcagatgccg cctttcgaca cgttgttgca ggagcagatc
     2281 tgtgctgttg ccggcagcgc agcgagcgcg ttggtcgggg gtgcacccgc acttgccacc
     2341 gctgccggaa acaacatggt ctcggggttt tcgggcaatg ccatcgcatt ggccaccgtc
     2401 tggtgccaca tcgagtagtc agacgtgtcg ccgaccagca ccgcgccgat gagcttcttg
     2461 ccctcggcat ccacaaccag tttcttgtag acctcttttc gctgatcgaa gaatgagtac
     2521 acgcgcgcac cgggagtcgc cgcgtgcgca tcgccgatgg agccgacctc cacgcccagc
     2581 agcttgagct tggtgctcat gtcggcgccc gcgaaggcct cgccttccgt gccgagaacg
     2641 cgtgcggccg cgatgcgcgc catctggtaa cccggcgcca ccaggccaaa tacccggcct
     2701 tcgtaggcag cgcactcacc aatggcataa atggattcat ccgacgtgcg gcagtcgccg
     2761 ttgatcgaga tgccgccgcg cggcgccacc tcgagtccgc aggcgcgagc cagttcgtcg
     2821 cgcgggcgaa tgcccgccga gatcaccacc acgtccgtct cgagggagcc gccgtcggcg
     2881 aaatgcatgc gatgacgtgc gctctcgccg tcgtctatgc gtgtggtgtt cttgccggta
     2941 tgcaccgcca cgccgagcgc ttcgatgcgc gaacgcagca ggccgccgcc gccttcgtcc
     3001 acctgcacgg ccatgagcct gggtgcgaac tcgactacat tcgtaaccag gccgagatct
     3061 ttcagtgctt tcgcggcttc cagtcccagc agtccgccac cgatcaccac gccgcgcgtg
     3121 gagttcttgc cggcctcact gatggcctct aggtcttcga tggttcgata cacgaacacg
     3181 ccgggccgct cgcgacccgg aatcggcggc acgaacgggc tcgaaccggt ggcgagaatc
     3241 aggatgtcgt attcgtattc ggcgccgctt ttggtcgtga cgcgcttgtt gacgcgatcg
     3301 atgcccgcaa ccggatcgcc ggaatacagc tcgatgccgt tcttgtcgaa aaattcacgc
     3361 gtggtgagcg tgagatcttc aacgctcgaa cccgagaaga acttggagag gttgacccgg
     3421 tcgtaggcaa gccgcggctc ttcggcgagc atggtgatcg cgaagtcgct ttggccatct
     3481 ttggtgagcg tttcaaggaa ccgctggccg accatgccgt gcccgaccac gacaattcgt
     3541 tttgacattg ctacccatcc ctcgtccgtg ttgaggttga atttgcaacg tctgtgccac
     3601 gcctttgaaa aggccgttct cgtcagccgg ggcgctgcct tcgcgccctt gcttcagcgc
     3661 ggtgcaaggg gttggggcaa gcgcttcagc agtgggcaac cggcgattca gggcttgggc
     3721 ggtggttcga cgaactcgag acccttgaac cgcagcgaca acgtcttttt gatctcagcg
     3781 cgcagctttt cggccctgcg catctcgctg tcgccaaaca ttcccgattt gatgttaatg
     3841 ccgactccga tctcgttgtc gcccaggaat gtgtacgtga ggaaaatgcc cctgcccatg
     3901 aacgcgcctg cccagtgcgg aggattcggt tcctgcggct tgagtccgcg ggctttcgcc
     3961 acctggctga tcgccgcgac gatctgatcc cgtggaatcg gcgccgatga ttcgacggcc
     4021 ctgatattga agtcgttgct gtgtcgcgga taggaggtcg ccacgacgcc gaaaagcgtg
     4081 gcggcaagca cgggcggcag aacccagcgc cgcagcgacg ccgggttcac gaagctgcac
     4141 ggctggctca cggcgaactt cccgcaggcg ggcatgatgg ccagcgccat caaatcccaa
     4201 tagtccaccg tgcggccgat tttcatcggc tgcacgtcgt tcatgaaggc aatgaatgcg
     4261 ccggacgcag ggctcttcca ccacaggaag gcgccgaaga tcgccaggta gatagcgcga
     4321 gcatgccgcg ggaacgccgc gaacaatggc atcgagacca cggcgatgcc ggcgaaatcc
     4381 gagagcttgc ccgtgacgag tcccgggtac gcctgcttca gcagccagtc gttgacgagc
     4441 aacacggcca gcgccaggag gaatggccac gtagtgaaaa ctgcgagtac acggtttcgc
     4501 atagcttgaa aacgtcacgc cgttaaagac agcccaaaaa agactgtgcc tggcacatga
     4561 gccgctccgt agggtggaat ctcgacgaag cccagcgagc ggtacagtgc ctgcgctgac
     4621 accatcgtcg caagggtgtc cagacgcaat tccgaatacc cgtactctcg cgcacaggag
     4681 atagccgctt cgccgagacg tttgccgagc cccaacccgc gcgctacggg gcgcacgtag
     4741 agccttttca tttcaattgt cgcgcgatcc agcgctctca gcgcaacaca tcccacggcc
     4801 tcgccgttta ccctggcgat gagcagatcc ccggtgggag gcgaatattt ccccggcagt
     4861 cccgcgagct ccgcactgaa tccctggtac tcgaggtcga tggaaatgga tgccgcgtac
     4921 tcaacgaaga gttctttcgc cagggcgatg tccgattcgt ccctcaccgg cacgatgctg
     4981 tggtccgggg cgttcatgta tcgctgagat gcggagccac gacttcgcga gtttgggcac
     5041 tcgaaaccac tggcacgatt tcgaaagtca cgcccgcacc gcgccactgc agaacccagg
     5101 cctgcaggag gcgcaggtca tcgcattcca tgagctgaaa gcaacgcgaa aagttcggtt
     5161 cgacccagct atcgatgtac ttgagaccgg acggaatcgc acggccgcta tcgcgcacac
     5221 gctggtagat gggcaccatg tcattgtcgc gaaatctttc aatgaccatg aagagcatgt
     5281 tggcctctca gaattcgacc tttccgaacc gcgcaatacc ctggttctgc cccggtgggt
     5341 cgagaaccga ttcatcgagt ggaatgggct cgccgagcca caccgcgaga gtggtgtggt
     5401 ccgcgagatc gtcgtcagtc agcggatgga tcagcgcgtt cagtcccgtc gcctcgagga
     5461 ttcgcgtgat atccggaacc gcggaagcca gaaactggat ttcgaattgc ggcatcgggt
     5521 gtgggccaac accggtttca tacatccttc cgaccatcac caggtttgcc agagtgccgc
     5581 tgacgagcat gtcactgagt tcctggtgca gccgctgcgc gacgtcccag gtgtcctcgt
     5641 tgtagtacac atgagcatgg tagggatcgg tcggatctgg agacttacga ctcacgcctg
     5701 agctcccggg tgcggtcagg tgaaggcgac aacgcatatg gaccatcgcc cagcatcgcc
     5761 tgcagcagca gcatgatgca ccagacgacg ggcagctcgg cgcctccccc gacaaaccag
     5821 aatcccttgc gggtggccca gaactgggct gcaccgagca tcatcggaac cgcgtacaag
     5881 cacacccagc gaacgcgaat gccgggaatg agcagcaacg cccccgcgat ctccgcggag
     5941 aggacgtacc agggaacaaa ccacgggtac ccgttgctcg caaagttcga ccaccactga
     6001 tcgaacccgc ccggaagcac cgcgaatttc cagtacaggt gcgcgacgaa cagagtgccc
     6061 agcgtcacac ggagcaatag cggtgcgtag aaagcggatc tggtgttcat ggcgcgcacc
     6121 gatcggagac taaggcacat gcaggaagat gtcgctaccg gtcatcaggc ccgttccctc
     6181 acaacccagg cgtcgccgag acgacgcgcc gagaccgtgc cgcgggtccc gagttgctgc
     6241 gcgaacagcg acacgcggaa ctcttcgagc atccagcgga attcgtcgtc gcccggcggg
     6301 cgctggccgc gctcggcctt gacccgctcg cggtagcgag tctcgagcgg ggcgatctca
     6361 gccgcgagct gcgcatcgcg ccgcggatca cggcccagct tctccagccg ctgttcgatc
     6421 gccttgaggt agcgagggaa ttctttcagc cgctcccgcg gcgtcgtcag caggaaaccg
     6481 gccggcgcca gccacgcgag ctgcgagcgg atgtcggcgg cagccgatgc cgccgccttc
     6541 ggcaaggcgg cctggattcg gcctaactgc tccagcgaac cctgcacgat ccgcgatatc
     6601 tcctgtgcgg gcagccccag gccaccacga agctcggcga tgcgcgcgtc gaagcccgcg
     6661 ccgctgcgca gtggctcatg gccttcgaga aatacggtca ttacgatgcg atcgagcagg
     6721 tcctcgcgca gatcgcgtcc ggcgacgaga tccgcgttca gcagcggatg ctgggcgagc
     6781 ccgcgataga caatttccgc ctgaacattg accggaagat ctcgtcgata cgacttgaag
     6841 tcgcgcgcca tcacgatgcg gatgagacgc gcgcagccac gctcgtgact gatgcgagcc
     6901 tcggccggcg tggcgaacac gcgcaggccc acgctttcgc cctcatcgac cagcgccggg
     6961 taaccggtct gcgcacgtgc ggcttgcacg tcctgcttgt cgggcagatc gccgaacacc
     7021 cagctcttcg ctccggtcgt gagctgcgac tgtttcgcat aatgctgctg cgaggcgccg
     7081 gcatgcttgc cgcgcaggct cgtgaggctg cgtgaacgcg cgatgacctt gtccgcatcg
     7141 tcgagcagca cgaagttcac gcgcagatgc ggcgaaagca ggtcctcgcg gaacgcgtcg
     7201 cgcggcacgg ggacaccacc cgtgcgcaac agcgcatcgg cgagctgcgc atacagcgat
     7261 ccgattcccc gctcgagcag cggcaacact cgcgcgaccg catcgggcac cggcacgaaa
     7321 tgcactcgca ggttcttcgg cagcgaccgg accagcgctt cgatcttctc gtcgagcagg
     7381 ccggggacca gccactcaaa ggcctcttcc ggcagctggt tcagcacatg cagcgggatc
     7441 agcgccgaga ctccatcgtc ttcgtgacca ggctcgaagc gatagcgcag ctggatcacc
     7501 agcggcccgg ccgagagatg gtcgggaaat tgctcggcat cgagctcggc ttcgccgggc
     7561 gagatgtcct gttccgtgag ccacagaatc tttggattct ttgcctcggc ctcgcggcgc
     7621 cagcgctcga aggccgcgcc ggtcgagatt ccctcgggaa ctctcgcgtc gaagaacgca
     7681 tacaactggg tttcgtcgcc gagcaggtcg acgcgacgtc ccttctgttg caggtactcg
     7741 gtgtccttga gcagcttcag gttgtgtgcg agaaacgggg cgcgcgagtc gtactccatg
     7801 tggaccagcg cgtgtttgat gaacagctcg cgcgcggcct tggggtctat ctgttcgtac
     7861 ggaacgcttc ggcccgaaga cagcaagagc ccgaacagtg tcacgcgttc atacaccgca
     7921 gcgcgtgagg cgcgacgctc ccagtgcggt tcatagtggt gccgtttcac gagatgcgcg
     7981 cccgcctgct cgatccacgc cggttcgact cgcgccacgt tgcgcgcgta gaccttggtc
     8041 gtctccacct gctcggcgct cacgatccat gcaggcttcg ccttgaactg gccggacccc
     8101 ggatggatga agagcttggt gccgttggcg ccgagatact cggcctgctc gcgccggttc
     8161 gcgacctgcg acagaagccc cgcgagcaac gcgcggtgga tagacgcgta ctggccgggc
     8221 tgtgcgttga gcttcagcgc gagttcgcct ttcacgacct ccatcacctg cccgtgcaca
     8281 tcgtgccact cggtgagccg gagataggaa aggaagtttt ccttgcacca gccgcgcaag
     8341 cgggcgcgcg agaggtgcgc gcgctgctct tcgaacgctc gccacagctt cagcaacgac
     8401 aggaaatccg attgctcatc gcgaagcggc gcgtgcttct ggtctgcctg agtctgcttg
     8461 tctgcgggcc ggtcgcgcgg atccggcacc gagagcgcag ccgcaatgat cgccacttcc
     8521 tcgagacagt gctcctcggc tcccgcgagc aggatgcggc cgaggcgcgg atccagcggt
     8581 agtttggcga gccgccggcc ggtgtcggtg agctgccctt cctccgacag tgcaccaagc
     8641 tcacgtagtg tgcgttgacc gtcgcgcaca aagcggccat cgggcggctc gacgaaagga
     8701 aaagcctcga tgtcacctaa cttcagcgcg tgcatctgca ggattaccgc ggccaggttc
     8761 gtgcgctgga tttccggttc cgtgaatgcg gggcgcgcgc cgaaatcctc ttccgagtac
     8821 aaccggatcg caacgccagg cccgacgcga ccgcagcggc cggaacgctg gctggcggag
     8881 gcttgcgaga tcttctcgat gggcaaacgc tgcagtcgcg tgcggtggct gtagcggctg
     8941 atgcgcgcca cgcccgtgtc gatcaccgcg cggatgccgg gaacggtaag tgaggtttcg
     9001 gcaacattgg tggcgagcac gacacggcgg cggcccgagg ggcggaatac acgttgctgc
     9061 tcttccgctg acagccgcga gtacagcggc agcacctcac aaccctgcgg atggtgcttg
     9121 cgcagcgatt cggcagtctc acggatctcg cgttcgccgc tcaggaacac caggatgtcg
     9181 ccggcctgct cggaccacag ttcatcaacg gcagaaagta tcgcgtcctg ctcgcccgag
     9241 gccgtctcgt cgtcttcttc gtccagctcg accggccggt agcggacttc gacgggatag
     9301 ctgcgcccgg acacattgat gatgggcgcg tcaccgaagt gcttcgagaa ccgctccggg
     9361 tcgatggtgg ccgacgtgat gaccagcttc aggtccggcc gcttcggcag cagccacttg
     9421 agatacccga gcaggaaatc gatgttcagc gagcgctcgt gcgcctcgtc gatgatgatg
     9481 gtgtcgtagg catcgaggaa gcggtcgccc tgcgtctcgg cgagcaggat gccgtcggtc
     9541 atcagcttga tcagcccctc ggggcggctg tgatcctgga agcgcagctt gtagccgacc
     9601 agctgcccga gcggtgtctt gagttcttcg gcgatgcgcg cgcccaccgc gcgcgcggcg
     9661 atgcgacgtg gctgcgtgtg gccgatgaca cccgccacgc cacgtccggc ttcgagacat
     9721 atcttgggca gttgcgtggt ctttcccgag cccgtctcac cgcacaggat gacgaccgga
     9781 tgtttttcga tcgcgcggcg gatctcgtcg cggttcgcgg agatgggcag ctcttccgga
     9841 taggagacgg ccgggacgct cgctgcgcgc gccgcgcgcg ccgccgtcga tacatttatc
     9901 ttctgcgcga gcgccgcttc cgcggccacg acgtcattgc cgcgaagacg cagcccgtgc
     9961 aggcgatcgc gctcgcgccg cagccgatgc tggtcgcggc gcaggcaggt gtcgatcgag
    10021 ggttcggggg cgggattcgg cggattcatg ggctcgggcg aggcccgggc gcggcgattc
    10081 gagggccgtg agtttacccg ggtttcttgt gacgcagcat cctcaggcgc aagatggggc
    10141 gggcatggcc gcatattcag tcgcgggatt tcacgtcatt accggcgttt gcgtgctcgc
    10201 cgccgtgcgt cacatgtcga ccgggctctg gcgccccatc gacaagacga atttgctgtt
    10261 cagcctgatg tgctgtttcg cggcgggcat ggcactgacg caggcgcagc tctatcaggc
    10321 ctggtcgatc gaagaacacc gctcggtgct caagtggaac atcacctttg ccctggcgtt
    10381 cttcctcgtc ttgccgtggt tcgcaacggc ggtcgcgggt cggcaacccg tgcggtggct
    10441 gatcgcctcg accatcgtgt tcattgtttt gatggtgctc aacctgatcc agccttttgg
    10501 cgtgcagctg gccggcatcg atcggatcga aaccaaactc atgccatggg gagaggagta
    10561 cgcgagccca gtgggcccga tcgcgcctgg cttctggttc gccatttcgg cggtgctgat
    10621 cgcaatctgc gatgccgtga ttcgattctg gtccggttgg cgccggcaaa aatccggctc
    10681 gagcctgttg ttggtgttga gcacgactgc ctacctcgtt gcgggcatcg aaggaattct
    10741 cgtccgggcg cagatgatcg acttcgttca cctgggcccg tttggcttct tcctgatggt
    10801 cattgcgatg agcctgacgc tgagttttcg gagtcggcag accctgatgg tttccgagca
    10861 gcgttttcgc gcgctggtgg agcagtcgcc gtttggcatt caggtcctgg cgagcgatgg
    10921 atcgacggtc caggtcaatc ccgcatggga acgcctctgg ggcaagagca ccccggagag
    10981 tcctcgcgtc gccgacgagc gtctgctgcc caccctcgag cgcgctttcc tcgggcacaa
    11041 gggcgagacg aatgccaggc ctgccgaagg cggcaagtgg attcgatcgt ttgcctaccc
    11101 gatcctggat tccttcgggg tcgtgcggaa cgtcatcgtc atgcatgaag acatcaccga
    11161 agagaagcgc gcagaagact cggcacgcgc ggtaaccgca gagctcagcc aggtcaaaca
    11221 cgccgtgttg cagcaggagc ggctgcgtgc gctgggacag atggcgggcg gcatcgctca
    11281 tgacatcaac aacgccattt ctcctgccgc gatgtacatc gaggctctgc tggagcagga
    11341 caagacgctg aattcgcgcg cgcgcgagcg tctgtccatc gtgcaacagg ccatcgcgtc
    11401 ggtggttcag accgtgtccc gcttgcgtga attctgccga ccgcgcgaag cgcaggcgac
    11461 acgcgaggac gtggatatca atgaggtggt caagcagtcc gtggtattga cgcaggcgcg
    11521 ttggaaaacc atggcgcagg gaaaaggcat cgccattcgt atggtaacga acctggcgga
    11581 cggtttgccc accatcaagg gcagcgccag cgagatccgg gatgcgatcg tcaatctcat
    11641 cttcaacgcc gtggacgcga tgccgcaggg gggcacgata accctgcaga cccgcgcact
    11701 gggcaatggc gcgagtatcg aggtgagcga cacgggcaca ggcatggatg aacaaacccg
    11761 ccggcgctgc atggaaccgt tcttttcgac caagggcgag catggcagcg ggcttggcct
    11821 cgcgatggtc tatggcatgc tgaaacgcca tggtggcgaa atcgagatcg tcagcgcgcc
    11881 aggcagtggc acgaccatgc ggctgcttct ttccactgac agcacccgca tggaacgccc
    11941 accagcaact tcgccgtcgc atgacaccgc ctccggccga actaccggcg cgcgaattct
    12001 gctcgccgac gacgaccccc tgctgctcga gtcactgcgc gccactctcg aaggggatgg
    12061 ccaccacgtg ctgaccgccg acgacggcca gagcgcgatc gacgcgttcg aagcggccga
    12121 caaggcgggc gcaccgtttg ccgccgtgat taccgacttc gtcatgccca tcgtcgatgg
    12181 gagacaactc tcgagcgtga tcaatcaggt gcggccggga accccggtga tcatgctcac
    12241 ggcctacgga aatcgaattc gcgcgcagga cgacacgctg ccgtacgtgg accacatcct
    12301 ggcaaagccc gcgaatctcg ccgagctgcg taatacgctc gagcgcctgt tgaaccgcta
    12361 gcgaagcact tcgctcaagg ccgcactcgg cgcatacggc ggcttgcgcg aaatgtcgcc
    12421 gcccgtatcc cagagcacgg gaaccatctg gcgggaaagt gcggcttccg caacggccga
    12481 catccagcgc acgcgagacg ccatctcttt cttgtccgtg acaccgaatt cgccgatgaa
    12541 agccggcaga tcgttgcgct tgcagaattc ctgcatcgcg tcgaacaggc gattgagctc
    12601 ggcgacgtcc gcggcacttc cccaggtgag ctgcatcttt cgccagcttt catccttggt
    12661 catgccagcg aattgccacg gtgtgtagta gtgcaccgaa atcagcagcc ggtgcggcac
    12721 gctgtccgtg ggcaacacgt actcactact cgtagtcctg gtgatgtcgg tggaataacc
    12781 cgtgacgatg aggagccgct tcgcgttgtt gccacccgtc ctgcgcaccg tgtcaatgaa
    12841 gagctgattc actcgagtca gtgtcgcgta ggccttcttc gtggaacccg tgccttcgaa
    12901 gttggtttcc tcattcagcg cctcgaagag caaccgctca tttcgatcgg cgaagtagct
    12961 cgcgatctgc ctccagtagg aaacgaattt gctttccgcg tccgcgctga aagtcgcaaa
    13021 tgttttcggg aatttctcct tggcgctcga gtcgatccag ccgccgtccc agtgaatgtt
    13081 cacgacgcag aacatgccgg ccgcggtgat ccaatcgacg acctcgccca cgcgggcgag
    13141 tttgcgcgcg tcgatgcggc cgttgtgggc gtacgtatcc caggccaccg gcacgcggac
    13201 cgtcttgaat cccagcttcg ccaggctctc gacaaacttt ttcgtgatgg gcggattccc
    13261 ccagccggtt tcccaggtcg tggtgttttc gagcgtgttg ccgatgttga cgccggtgcc
    13321 catcaacttc gccgcggtcc atgcatcgag atcttccgcc catgccgcag acgacaggag
    13381 cacgcccagc atgacggcca acagactcgc acattgtttt ttcatgcgcg gactctagcg
    13441 gccggcgtca aaagaatctc tgcagaaacg ccgccgcgtc cgccgagagg ccgacgatct
    13501 tgttcgtcgc ctggcgatag aacttgaagt tcagttcggt ctccgggcgg ccgtctttcc
    13561 agttcgagaa cggcttgagt tcacttcggc cgaggcgctt cttcacaaga gcgctgcgcc
    13621 tgacggcgat actcacgcgc ggagtttggc gctcgattcc gggtttccgt ggcgttgcct
    13681 tggccgcggt gaccacgccc cgcgcgatca gcccgggacc gccctcgttc tcactcgcga
    13741 tgatgaagat gacgtcgccc ttggcgatac gcttcccgcc gtacatcgtc ttctgcgcgt
    13801 tgaaggtgaa cgccttttcc ttgaggttcc tgatttcgac tttgattgcg tacatggctc
    13861 tcaggcccgg ttgagaagca tatggctaac ttgcgcgcgc gaagcgtccc ggcgattggc
    13921 ccacgtaccg gctgaacgcc gtgctgaagg tgcttgccga gccgtagccg actcgctcgg
    13981 caacctcggt gagcgccaga tcctgtcgtc ggagcaaatc cttcgcgacg gccattcgcc
    14041 aggcgagcag atattccatc ggtggctgcc ccacggcgcg cgtgaagcgc tcgaagaatg
    14101 tcgaacgcga aagcgctgct ttcgttgcga gctgcgctac cgtccatgat cgctctggat
    14161 tggcgtggat cacccgcatc gcatgcgcaa cgcgcgcatc ggccaggccg cgaagcagcc
    14221 cgggcggtgc atcctcgccc gaggtggccc tcaatgcctc aacgagcaga acttcgacga
    14281 gccgggaaag cacgagctca cgccctgatc tttgcccgct cgactcttcg tcgacgagtc
    14341 gcaccagcgt cgaaagccgc tcgacaccgc gcacgtgcac gagcgagggc aacagtgaca
    14401 ccagcagcgc cgcatccggc gagccgaata cgaaatagcc ccctaacaag cgcacgtcgg
    14461 gacggccgcc cttcctgcca tgacgaactt cacccaacga accggccgcc ttcttcgaat
    14521 cgatgcgaac cggtgttgcc ggctcgtagc tggacatcac gaaacccggg gttgccggca
    14581 gcagcacgaa gtcacccgct tcaagagtca gtggcttctg cttgtcgacg gccaggaggc
    14641 agccgccttc gagcacaacg cagaaactcg ggtgaccgaa gtccgagtaa cgaactcccc
    14701 agcgacctgc gccgctgatg cccttcgaga acacagcgcg tggctggagc agcgtgatga
    14761 cttcggccag tgggtcgttc atgcccggac gatcgccaat gaattatgga cgctgcattg
    14821 tagagcgtcc ggccggccat ctctatcgtg cccctaccaa ccacgaagga gttcccatga
    14881 aaaccgtact catcaccggc tgctcttccg gctacggcct cgaaaccgcc cgtcacttcc
    14941 tgtcccatgg ctggaaagtg atagcaacca tgcgtacacc gcgggcgaac atcctgcctg
    15001 cgtcggaaaa tctgcgcgtg atcgcgctcg atgtgacgcg tgcggagagc atcgccgccg
    15061 cgctcgaagc cagcggaccc atcgatgtgc tcgtgaacaa tgccggcatt ggactcttcg
    15121 gcgctttcga agcgacgccg atggccacga cgcgcgaggt gttcgagacc aatacgtttg
    15181 gtgcaatggc gatgacgcag gctgtgctgc cgcagttccg tgcgcgccgt tcaggcttga
    15241 tcgtgaatgt gacatcgagc gcaacgctgg cgccgatgcc aatggtcgcg gtgtacaccg
    15301 cgagcaagat ggccatcgaa ggattcaccg cgtcgctggc gttcgaactg gagtcgttca
    15361 atgtacgcgt caagctcgtc gagccgggtt acggaccgac cactggattc tccagcaaca
    15421 gcggttcgcg catgcagggg ctcattccgg aagcctatgc gccattcgcg cagaatgtgt
    15481 tcgcatcgtt cacgcagccg ggcgcggtga caaaggagtc agatgtcgcc gaagttgtct
    15541 ggcaagcggc gaacgatgcg tcgacgcggc tgcgctatgc ggcaggggcg gatgcggagg
    15601 ctctggcaaa gtctgcgcgc catgtctagc tcagggcgat gacatcatcc gcgtcgatca
    15661 cgtgaaccgt ctcgcccgcc aacgcgtaca acttttttcc gcgtccgacg atgcgttcga
    15721 tggacaccgg gttttcattg atgtcgacca gccggaacgt gtcgaccagg gtcgcctttt
    15781 catcgcgatc gagctcgacg aggtgcagca gatccggctc gttgtatgca cctgcgaata
    15841 ccacgtag
//
LOCUS       DASQJS010000225         3575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1698601, whole genome shotgun sequence.
ACCESSION   DASQJS010000225 DASQJS010000000
VERSION     DASQJS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698601"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VEW08_09175"
     CDS             complement(<1..467)
                     /locus_tag="VEW08_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HYJ40949.1"
                     /translation="MKTNLKPQSAPEGNAFISDLSALRSRAREQMMKGAVTPSFPEAD
                     RKAIVELLNTALATEIVCVLRYKRHYYSTHGIRAKFVSAEFLEHAKEEQAHADQIAER
                     IVQLGEDPDLNPATLLSRSHSEYDEATSLRQMLEADLFAERIAIESYREMINF"
     gene            complement(472..711)
                     /locus_tag="VEW08_09180"
     CDS             complement(472..711)
                     /locus_tag="VEW08_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40950.1"
                     /translation="MISPLERARLLDAEMRLQRAWLELSLREMSSAARGSPWALLQPL
                     KLGKVGMSLLKHRSLWLAAVSLLLNLFRRRAHKEK"
     gene            complement(695..1198)
                     /locus_tag="VEW08_09185"
     CDS             complement(695..1198)
                     /locus_tag="VEW08_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40951.1"
                     /translation="MEPQNDAPAAPPEEAPEEEEPGTARALTIHLLRMLETRVDAAGI
                     ALQAEMHSFSSRLQLRVIAAALLFIALWGGIVLLAIVLPPHLRVPVLAAVIAAFVIGG
                     VWAHLAANRAVSSRDIGSMTWFLDSLKLDMEVLSRSLAQSRAARQAPPAATPDVSTRS
                     DQNDLAA"
     gene            complement(1198..1548)
                     /locus_tag="VEW08_09190"
     CDS             complement(1198..1548)
                     /locus_tag="VEW08_09190"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HYJ40952.1"
                     /translation="MPEPAMKPEPKITVDPAIEELNRIVEQAESLLRSLGAEGGEAAE
                     AVRERVSQTLEQARSKLKATASSAEEVVESLADRADGFVRSNPWQAVAIAALLGGVVT
                     LLITKSTSKLASRQ"
     gene            1859..3178
                     /locus_tag="VEW08_09195"
     CDS             1859..3178
                     /locus_tag="VEW08_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HYJ40953.1"
                     /translation="MQFARTLPLSRSRTPVDMESADGMVSRAERVRVSRKLSTTGDTT
                     LLDFHLAAMQDIGAPPLITGNQVRLLIDGPSTYEAMFAAIEKARHYVLVESFIFEEAA
                     AGERTLSALLAEVTKRGVRVSVLYDAVGSMTTDEAFLAGLKDAGIALCAFNPLNPLDD
                     RFSGVNHRDHRKIVVVDGEHAFAGGVNFSQAYHIASKQARRRGLSQQKALHQGWRDTH
                     IAIQGTAARHLEDLFRGTWRDAECRGEAPPVTAPAVAAGNTLVQIVASSPDDETNDIY
                     ATLLSVLAYAQRSVDVTMAYFVPDDTLEKSIKDAAQRGVTVRIILPSYSDFSGVFYAG
                     RAHYDELLESGVKLYELENAFLHSKSIVVDGVWSSIGSTNFDWRSFVHNNEISVCVIG
                     EDFAAQMTHMFNTDLADSKEITLAAWKKRGFSERFKEWMWIPIQYWL"
BASE COUNT          644 a         1140 c         1125 g          666 t
ORIGIN      
        1 atgaaattta tcatttcccg gtaactctcg atggcgatgc gttcggcaaa caggtctgcc
       61 tcgagcatct ggcgcagcga agtggcttca tcgtattccg aatgggatcg tgacagcagc
      121 gtcgccggat tgagatcggg atcttcgccg agctgcacga tgcgctcggc gatctggtcg
      181 gcatgtgcct gttcttcttt ggcgtgctcg aggaactcgg ccgagacgaa cttcgcccga
      241 atgccatggg tcgagtagta gtgccgcttg taacgcagca cgcagacgat ttccgtggcg
      301 agggccgtgt tcagcagttc gacgatggcc ttgcggtccg cctcgggaaa gctgggtgtg
      361 accgcgccct tcatcatctg ttcgcgcgcg cgcgagcgca gcgcactcag gtcggaaatg
      421 aaggcgttgc cttcaggtgc cgattgcggc ttgagattgg tcttcatgat ttcacttctc
      481 cttgtgcgcg cggcgccgga acaggttgag gagcaagctg accgccgcga gccacagcga
      541 tcgatgtttg agcagcgaca tacccacctt gccgagcttc agcggctgaa ggagtgccca
      601 gggcgaaccg cgtgcggcgc tcgacatctc gcgcagcgaa agttcgagcc acgcgcgttg
      661 caggcgcatt tccgcgtcga gcagtcgcgc ccgttcaagc ggcgagatca ttttgatcac
      721 tccgcgtgga tacatccggc gtagccgccg gcggcgcctg tcgtgcggca cgggactgcg
      781 caaggctgcg cgacagcact tccatgtcca gtttcaggct gtcgaggaac caggtcatcg
      841 aaccgatgtc gcgcgaggac accgcgcgat tcgcagcgag gtgtgcccat acaccgccga
      901 tcacgaaagc ggcaatgacc gcggccagta ccggaacgcg caggtgtggc ggcagcacga
      961 tcgccagcaa cacgatgccg ccccacaatg cgatgaacag caacgccgcg gcgatcacgc
     1021 gcaattgcag tcgcgatgag aaggaatgca tttcggcctg cagcgcgatg cccgccgcgt
     1081 cgacgcgcgt ctcgagcatg cgcaacaggt gaatggtgag tgcgcgcgcc gtgccgggtt
     1141 cctcttcctc cggtgcttcc tccggcggtg ccgccggagc gtcgttctgg ggttccatca
     1201 ctgacgactt gccagcttgc tggtcgactt cgtgatcagc agtgtcacca cgccgcctaa
     1261 caacgccgcg atggcgacgg cctgccaggg attgctgcgc acgaagccgt cagcgcgatc
     1321 cgcgagcgac tccaccacct cctcggcgct gctggcggtg gccttgagct tcgacctggc
     1381 ctgttccagc gtctggctga cccgctcgcg caccgcttcg gcggcttccc cgccctccgc
     1441 gcccagcgag cgcagcagcg actcggcctg ctcgacgatt cgattcagtt cttcgatggc
     1501 tgggtccacg gtgattttcg gctccggttt catggcgggt tcaggcatag gtgttccttt
     1561 gaatcacaca atggaccctg tcttgccggg tcacattcgg atttgcagcg cacgggttgt
     1621 aggattttgc cgacgccacg atggcacacc tgccgggcgc gccgcaacgc gcaggagagg
     1681 gatacttcgg gccgtctagt ggagatgtgg aagattgacg cgacgagccc tcccctcgaa
     1741 gatttcgcgt catgcgacca ccgggccatc cgtccatcgg cctcgccgcc ggtggacggc
     1801 gtggctcgca ccgtcactga tctgcgccgc actcgccggc tgccacgtcc ttcccgaaat
     1861 gcaattcgcg cgcacgctgc cgttatcgcg ttcgcgcacg cccgtcgaca tggaaagtgc
     1921 cgacggcatg gtcagccgag ccgagcgcgt ccgtgtgtcc cggaagctca gcacgacggg
     1981 cgacaccacg cttctcgact tccatctcgc cgccatgcag gacatcggcg caccgccgct
     2041 catcaccggc aaccaggtgc gcctgctcat cgacggcccg agtacctatg aggccatgtt
     2101 tgcggccatc gaaaaggcac ggcactacgt cctggtcgag agcttcatct tcgaggaggc
     2161 agccgccggc gaacgcacgt tgagcgcatt gctggcggag gtcaccaagc gcggcgtacg
     2221 cgtctccgtg ctttacgatg cggtggggtc gatgacgacc gacgaagcgt ttctcgccgg
     2281 cctcaaagac gccggaatcg cactctgcgc cttcaatcca ctgaatccgc tcgatgatcg
     2341 attttcggga gtcaaccatc gcgaccaccg gaagatagtc gtggtggacg gtgaacacgc
     2401 attcgccggc ggtgtgaact tcagccaggc ctatcacatt gcctcgaagc aggcacggcg
     2461 ccgcggactg tcgcagcaga aggcgctgca ccagggatgg cgcgacactc acatcgccat
     2521 ccagggcacc gccgcgcgac atctcgagga cctgtttcgc ggcacctggc gcgatgccga
     2581 gtgcaggggc gaggcgccgc cggtgaccgc gcccgccgtc gcagccggca atacgctggt
     2641 gcagatcgtc gccagcagtc ccgacgacga aaccaacgat atctacgcca cgctgctttc
     2701 ggtgctcgca tacgcgcaac gcagcgtcga tgtgaccatg gcctacttcg ttccggacga
     2761 cacgctcgaa aagtcgatca aggatgcggc gcagcgcggt gtcacggtac gcatcatcct
     2821 gccgtcatac agcgatttct ccggggtgtt ctacgcgggc cgcgcccact atgacgagct
     2881 gctcgaaagc ggcgtcaaac tctacgagct cgagaacgcg ttcctgcact ccaagagcat
     2941 cgtcgtcgac ggcgtgtggt cctcgatcgg ctccacgaat ttcgactggc gcagtttcgt
     3001 gcacaacaat gagatcagcg tgtgcgtgat cggcgaggat ttcgccgcgc agatgacgca
     3061 catgttcaac acggaccttg ccgactcgaa ggaaatcacg cttgccgcct ggaagaagcg
     3121 cggtttcagc gaacgattca aagagtggat gtggatcccg atccaatact ggctatgaga
     3181 tgggggcatt cctcgtttgc tggcaagagg gggcggggct cgtgtgtgga tggtcgagcg
     3241 acgggcggcc ctgcatgcga tgagactcgc ttttcgccgg gcccttcgtt cggtgtggca
     3301 tcgccagttt gcgggccgcc gacacacgcg aagacactct ggcgtagttt gatttgaaga
     3361 tggcgaagag tcccgaagac gcagggatcg gcttcgtgcc taacgaggcc aggtatcccg
     3421 gcccactcgc tcatcgcatg cagggccacc cgtcgctcga tcgtgcaccg acgcgccgcg
     3481 actccgttcg ccagaaaaga ggcgtgtaac cccacgcggt gggaagacct caagtccgtg
     3541 cgtctcttct cttaccaccg ccgcggggaa gcggg
//
LOCUS       DASQJS010000226         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2330159, whole genome shotgun sequence.
ACCESSION   DASQJS010000226 DASQJS010000000
VERSION     DASQJS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2330159"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            427..837
                     /locus_tag="VEW08_09200"
     CDS             427..837
                     /locus_tag="VEW08_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40954.1"
                     /translation="MKNQRALIALTIVNLALLLLTLTQQMRPAFAEGPAPMLRGSGLE
                     IVDGKGRVRASITVLPAGKSQAGDAYPETVLLRLITERGRPSVKVSTSEEMSGLTLAG
                     PTNTSDTYVMLQAKGEVSSLKMRNEDGHEQVLKP"
     gene            complement(842..2935)
                     /locus_tag="VEW08_09205"
     CDS             complement(842..2935)
                     /locus_tag="VEW08_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ40955.1"
                     /translation="MNPSGKSAAVLVALLALGAQALASPDAEVLVAWNARVLAAAESE
                     DRLLTLKGVRTAAMMHLAIHDAINCVERRYDPYAYRANGKGADALAAATQAAFAVAVD
                     QYPDRLADWDAQRARWLGQIDDGPRKEKGIALGNAAAAAILVRRANDGWNSEASYQFH
                     PMAPGVYAEFSEHSGTPQGFVFGAGWAMARPFALRSPSQFRSPPPPDIGSREYTRAFD
                     EVREFGRYQSRSRTADQTHLAMWWKDFAENSHNRLARDLVTNEQPDLWESARLFALLN
                     VSIMDAYISVFDNKFFYNHWRPYTAIRWAEHDGNPATVAEPGWNNTHRHTYAFPSYPS
                     AHGTACAAAMTVFADTFGENRYFRMSTPQVAAAGPMSEKISMKPPTRSFDSFTEAARE
                     CAISRVYLGIHFRYDSAEGVRLGRRIGEYIISQYLRASKWKRAAIGSVELEYQLRGAG
                     EPVVFVHAGIFADWFEPLLNQPALRKAHRTLTYHRVGYAGSSRANGNATILAQAQQLG
                     ALLDNLNLRRVHLVGHSSGALIAMQLALDAPDAVRSLTLLEPALSIAGSSSPVIASAV
                     SIYRSGRREEAVDAFMRAVGGNDYRATLERVLPRAMDQAIADADGFFEHELPAVRAWS
                     FGDSDARRLRAPVLLVMGGRSDDVSPIWRQRHELLLRLLPETETFVLPGATHLLQLQN
                     PAALADRIAAFVASH"
     gene            3023..>3207
                     /locus_tag="VEW08_09210"
     CDS             3023..>3207
                     /locus_tag="VEW08_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HYJ40956.1"
                     /translation="MKRGYGQYCPLALAAELLCERWTLLVISRVIDGCTQFNAIHRGV
                     PRISPAMLTKRLRQLEHA"
BASE COUNT          591 a         1008 c         1033 g          575 t
ORIGIN      
        1 cacaaggtct ggtggcgttg tggcgggaag ccctgcttgc acaggccgtg ctgcggggca
       61 agacacacgg ctacaggcat cacccccaac tcgagcgttt tacgtctcaa gcgtcaccgc
      121 gcctggcgat caacgcatat ctcggcgcca tatttgaaga ggcgacgcgg cgaggctacg
      181 agttcgaccg tggaaaaatc gggccgcttc gggcagtgcc ccgtatatcc gtgtccaagg
      241 ggcaactcct ccacgagtgg cggcacctgc ttcaaaagct tgccgcgcgc agcccggcgt
      301 tgcactcgag gtggcgaaac gtttcgagcc cggcgcatca ccccctgttt cgcacgcggc
      361 caggatccaa ggcgtcatgg gaacgcagct gagcgacacc tgtccgaatt catcggagga
      421 tcacacatga aaaaccagcg cgcactcata gctctcacaa tagtcaatct ggcactcctg
      481 ctccttacgc tgacacagca gatgcgcccc gcgtttgccg aaggccctgc gccaatgctt
      541 cgaggaagcg ggctcgagat tgtcgatgga aaggggcggg tgcgcgcaag catcaccgtt
      601 cttccagcgg ggaaatctca ggctggtgat gcctatccag agacggtatt gctcaggctg
      661 atcacggagc gcggacggcc ttcggtgaag gtaagcactt cagaggagat gtccggactg
      721 actcttgccg gcccgaccaa taccagcgac acgtacgtca tgcttcaagc caagggtgaa
      781 gtcagttcgt tgaaaatgcg caacgaagac ggccacgagc aggtgctcaa gccgtaggtc
      841 actagtggct ggcgacgaat gctgctattc gatcggcgag cgccgcggga ttctggagct
      901 gcagcaggtg cgtcgcaccc ggcaacacga aggtttccgt ttccggcagg aggcggagca
      961 gcaattcatg ccgctgccgc cagatcggtg agacgtcgtc gctgcgtccg cccatcacca
     1021 gcaacaccgg cgcgcgcaat cgccgtgcgt cgctgtcccc gaaactccac gcgcgtaccg
     1081 ccggaagctc gtgctcaaag aaaccgtccg catcggcaat ggcctggtcc attgcccgcg
     1141 gcagcacacg ctccagcgtg gcgcggtagt cgttgccgcc gaccgcgcgc atgaaggcat
     1201 cgacggcttc ctcgcgccgc cccgatcggt agatagagac agccgaggcg atgacggggc
     1261 tggagcttcc cgcgatggac aaggccggct cgagcagcgt gagtgatcgc acggcatccg
     1321 gtgcgtcgag cgcgagctgc atcgcgatga gcgcgccgga ggagtggccc accaggtgca
     1381 cacgacgcaa gttcaggttg tcgagcaggg cgccgagctg ctgcgcctgc gcgagtattg
     1441 tggcgttgcc gtttgcgcgg ctgctgccag cgtagccgac gcggtggtac gtcagcgttc
     1501 gatgcgcctt gcgcagcgcg ggctggttca gcagcggttc gaaccagtcg gcgaatattc
     1561 ccgcgtgcac gaataccacc ggttcacccg caccgcgcag ttgatattcc agctcgaccg
     1621 aaccaatggc agcgcgcttc catttcgatg cgcgcaagta ttgcgagatg atgtactcgc
     1681 cgatgcgcct gcccagccgc acgccttcgg ccgagtcgta gcgaaagtga attcccagat
     1741 acacgcgcga gatcgcgcat tcgcgcgcgg cctccgtgaa actgtcgaag gagcgcgtgg
     1801 gcggcttcat cgaaatcttt tccgacatcg ggcccgcggc ggccacctgc ggcgtcgaca
     1861 tcctgaagta gcggttctcg ccgaaggtat ctgcgaacac cgtcatggcc gccgcgcagg
     1921 ccgtgccatg ggcggacgga tatgacggaa acgcatacgt atgccgatgc gtattgttcc
     1981 agccgggttc cgccacggtt gcgggattgc cgtcgtgctc tgcccagcgg atcgcggtat
     2041 acggccgcca gtggttgtag aagaacttgt tgtcgaacac gctgatgtac gcatccatga
     2101 tgctcacgtt gagcaacgcg aacaggcgcg cggactccca caggtcgggc tgctcgttgg
     2161 tgacgaggtc acgcgccagc cggttgtgcg aattctcggc gaaatctttc caccacatcg
     2221 cgaggtgtgt ctggtcggcg gtgcgcgaac ggctttgata gcggccgaac tccctgacct
     2281 cgtcgaatgc acgcgtgtac tcgcggctgc cgatgtcggg tggtggcggc gaacgaaatt
     2341 gcgagggcga gcgcagcgca aagggtctcg ccatggccca gcccgcaccg aagacgaagc
     2401 cctgaggcgt gccgctgtgt tcgctgaatt ccgcatatac acccggcgcc atcggatgga
     2461 attggtatga cgcttcgctg ttccatccat cgttcgcgcg ccgcacaaga atggcggcgg
     2521 cagcggcgtt gcccagcgcg attcctttct ccttgcgggg tccatcgtcg atctggccga
     2581 gccaccgcgc ccgttgcgcg tcccagtcgg cgagtcggtc cggatactgg tctacggcaa
     2641 cggcgaacgc tgcctgcgtc gccgcggcga gcgcgtcggc gcccttgcca ttcgcccggt
     2701 aggcataggg gtcatagcgc cgctccacac agttgatggc gtcgtgaatg gccaggtgca
     2761 tcattgccgc ggtgcggacg cccttgagtg tgaggaggcg gtcctccgat tcggccgccg
     2821 cgaggacacg cgcgttccag gccaccagca cctcggcgtc gggactggcc aatgcctgtg
     2881 caccgagtgc cagcagagcg acgagaacag cggcggattt tccggaaggg ttcatggggc
     2941 cgagcgtacg aacgctgccg cgaagcgact actatcgttt ctgtagtagt cagggcgtgc
     3001 cgcccctgca aggatggcca acttgaaacg cggatatggt cagtattgcc cgttggcgct
     3061 cgccgccgag ttgttgtgcg agcgatggac tttgctcgtc atcagccgcg tgatcgatgg
     3121 atgcacgcag ttcaatgcca ttcaccgcgg cgtgccgcgc atctcccccg cgatgctcac
     3181 caaacgcctg cgccagctcg aacacgc
//
LOCUS       DASQJS010000227         5121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2330842, whole genome shotgun sequence.
ACCESSION   DASQJS010000227 DASQJS010000000
VERSION     DASQJS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2330842"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VEW08_09215"
     CDS             complement(<1..603)
                     /locus_tag="VEW08_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HYJ40957.1"
                     /translation="MSTTPKIALVAGATGLVGGFLLKTLLDAPDYTRVYALTRRLIGK
                     EHPKLANRVVIFDRMAEQLKGLVAQDAYCCIGTTIAEAGSQEAFREADVDAVLLFARA
                     ARAAQATRFVVVSSVGASSASKKFYLRTKGEMEEAVTNLGFASVDILQPSLLLGPRKT
                     LRLLEVTAQVFAPLINPLLTGAREPLRAIPAETVARAMLGA"
     gene            complement(656..1315)
                     /locus_tag="VEW08_09220"
     CDS             complement(656..1315)
                     /locus_tag="VEW08_09220"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HYJ40958.1"
                     /translation="MPSYVAVMHNARVLLIIDLIGVFVFALAGATAAVRQRLDLFGVL
                     VLSFATATTGGILRDLLIGAVPPAAFADYRYLVAAVLAGLVTYVWHRAIERLRNPVRV
                     FDAAGLGLFAVAGTQKALAAGLHPVMAALLGMLTGIGGGVMRDLLLARVPVVFQSDIY
                     ALAALAGATVVVLGNWLHWPQVPTAIAGALLCFGLRILAMWFHWHLPSARYEKPSEKD
                     S"
     gene            1426..2271
                     /locus_tag="VEW08_09225"
     CDS             1426..2271
                     /locus_tag="VEW08_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40959.1"
                     /translation="MHITSYPRVAAWLPKVMLILASFAGSAQAVEFDEKIRAPMNRGG
                     AELKTQAESYAASFARLSTASPAEMVTNGALSQERFDLEWQLTRALEDKRPMEDLSAL
                     GLVKNENGFRIDYNAFPQWQPFPEKLASLMPTMSMDAAGPLLVARGFRDSDVVALRNY
                     LETHDLDMETSSRTLPIAISFSRVVRKYDKIKRPVGKDLVFSFLYQRDKTGVEARRAW
                     SEGLIRTLDDQRVRVLQSYLSEIQGVGYWSPSDAEAGVANLLATMRLPDYEQRATAEA
                     AGGAP"
     gene            2268..2810
                     /locus_tag="VEW08_09230"
     CDS             2268..2810
                     /locus_tag="VEW08_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40960.1"
                     /translation="MKISQLTPRCRATLLGAAAMLGAPQLTSAIQMNVYVSTPSQRGT
                     DSAFYLGHTVMASTPFNVLNAGGNYTVQCNHTATLPITGERTLASTTYGFDRNTLTVT
                     IPAQQPAIRNLSGWLQIPDYTALSCNYRWTAFATEGGYSIGAGGISFPIGNGTVRDGD
                     TVDFTMYRPSREDADGGCIP"
     gene            2853..3473
                     /locus_tag="VEW08_09235"
     CDS             2853..3473
                     /locus_tag="VEW08_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40961.1"
                     /translation="MKNLYRIFAIAVFATASQLCNAWGSCTPIVIDLGNNGINLGPAG
                     VGVHFDVNADGIRDHVQWVRQGGDEGFLAFDRSGNGVVDDGAELFGVGTPLVLEGRNA
                     RNGFVGLAQYDSRQLGGNDDGLITEADAIWPQLRIWIDANADGVSTRDEMRTLRSFGL
                     TALDTIPKIRKYVDAAGNVIPYWAWAQQRARPGRALMVDVFFVVLP"
     gene            complement(3486..3899)
                     /locus_tag="VEW08_09240"
     CDS             complement(3486..3899)
                     /locus_tag="VEW08_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYJ40962.1"
                     /translation="MAAKTLTQVLAELRSCGGTRLRRSARIPDAAPLIARLVGPCRTL
                     AVEERAHGLAFRLQGATDSAAPFTFTCVLHLPGAVVLTAAEQAVADFLCEGRTLAQVA
                     RLRGVSPNTVKSQVRQIFRKLNVESRVALVRMLSP"
     gene            complement(3970..4521)
                     /locus_tag="VEW08_09245"
     CDS             complement(3970..4521)
                     /locus_tag="VEW08_09245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HYJ40963.1"
                     /translation="MPKQIGVVVGSLRKESINRKLANALIKLAPPDFTCKDLRIGDLP
                     LYNQDDDKSQAPEVQRLKSELRAVDAVIFVTAEYNRSIPGVLKNAIDHASRPYGQSAW
                     AGKPAGVIGASIGAISTAVAQLHLRTILAYLDMPTLGQPEAYLQVKDGFFDEAGNIAN
                     ADTRKFVHGWMDKYVAWVKKFTA"
     gene            4636..4980
                     /locus_tag="VEW08_09250"
     CDS             4636..4980
                     /locus_tag="VEW08_09250"
                     /inference="COORDINATES: protein motif:HMM:NF018991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1475 family protein"
                     /protein_id="HYJ40964.1"
                     /translation="MHKRALQLLFAFILVSLTAFSLWASTRQPVWEWGGLTTPPDNLW
                     TIATLIDAYYGFITFYAWVLWKEVRALPRVLWFIAIMLLGNIAMSAYVLRQLARLEPA
                     DSMATLLSARNA"
BASE COUNT          974 a         1641 c         1588 g          918 t
ORIGIN      
        1 cgcaccgagc atcgcccggg ccacggtctc cgcagggatg gcgcgcagag gttcgcgcgc
       61 acccgtaagc aatgggttga tgagcggcgc gaagacctgg gcggtgacct cgagcaaccg
      121 caatgtcttg cgcggtccca acagcagact cggctgcagg atgtcgaccg acgcgaaccc
      181 gagattggtg accgcctctt ccatctcgcc cttggtgcgc aggtagaact tcttcgaggc
      241 cgagctcgcg ccgacggaag acaccaccac gaaacgcgtg gcctgcgccg cgcgcgccgc
      301 gcgcgcgaac agcaacacgg cgtcgacatc cgcttcacga aatgcttcct gcgaacccgc
      361 ctcggcgatc gtcgtgccaa tgcagcagta ggcatcctgg gcgaccagac ctttgagttg
      421 ctccgccatg cgatcgaata tcaccacgcg gttggcgagc ttcgggtgct ccttgccgat
      481 gaggcggcgg gtcagcgcat agacgcgcgt gtagtccggg gcatcgagca gggtcttgag
      541 gagaaatccg ccgacgaggc cggtggcgcc ggcaaccagc gcgatttttg gtgttgtgct
      601 cacgccatga agcttaacaa gattcgcccc ggtgtcctta tcaggagggt ccttgtcagg
      661 agtccttctc agacggcttc tcgtagcgcg cgctcggcag atgccagtgg aaccacatgg
      721 caaggatgcg caggccgaag cacaacaagg cgccggcgat tgccgtgggc acctgcggcc
      781 agtgcaacca attgcccagc accacaaccg tggcgccggc cagcgccgcc agcgcgtaga
      841 tgtcggactg gaacacgacc ggcacgcgcg ccaggagcag gtcgcgcatc acgccgccgc
      901 cgatgcccgt gagcatgcct aacagggccg ccatgacggg atgcaacccg gccgccaagg
      961 ccttctgcgt gccggcgacg gcaaacaacc ccaggccggc ggcgtcgaaa acacgaaccg
     1021 ggttgcgcag gcgttcgatc gcacgatgcc agacgtaggt gaccaggccg gcgagcaccg
     1081 ccgcgaccag gtaccggtag tcggcgaagg ccgccggcgg caccgcgccg atcagcaggt
     1141 cgcgcaggat tccaccggtc gtggccgtcg cgaacgacag gaccagcacg ccgaacaggt
     1201 ccaggcgctg gcgtaccgcg gccgtcgcgc cggcgagcgc gaagacgaat acaccgatga
     1261 gatcgatgat gagcagcacc cgcgcattgt gcatcacggc gacgtagctg ggcattcgcg
     1321 tcgcttttgg cgactggtca cccgctcggg tgatgcggcg gaaacggcgt tgactatgct
     1381 ttgagcggga aggaatccct cacctaacga acaaggagtg aatacatgca tatcactagc
     1441 tatccacgtg tcgcggcgtg gctgcccaag gtgatgttga tactcgcgag tttcgcgggc
     1501 agtgcccagg ccgtcgagtt cgacgaaaag atcagggcgc caatgaacag gggcggtgcc
     1561 gagcttaaaa cgcaggccga gagttacgct gcaagcttcg cgcgactgag cactgcctca
     1621 ccggcggaaa tggtcacgaa cggggcgctc tcgcaggagc gtttcgatct cgaatggcaa
     1681 ctcacccgcg cgctggaaga caagcggccg atggaagacc tgtcggcact cggtctcgtg
     1741 aagaacgaaa acggcttccg catcgactac aacgcatttc cgcaatggca gccgtttccc
     1801 gaaaagctcg cctcgctgat gcccaccatg agcatggacg cagcgggacc gctgctcgtc
     1861 gcccgtggtt ttcgtgacag cgacgtcgtg gcgctccgga actatctcga aacgcacgat
     1921 ctggatatgg aaacctcctc gcgcacgctt ccgatcgcaa tcagtttcag cagggtcgtc
     1981 aggaaatacg acaagataaa gcgcccggtc ggcaaagacc tcgtgttctc gtttctctac
     2041 caacgcgaca agaccggagt ggaagcgcgg cgcgcctggt cggaaggtct catccgcact
     2101 ctcgacgacc aacgcgttcg cgtgctccag tcctacctct cggaaatcca gggcgtcggc
     2161 tactggtcac ccagtgatgc ggaagccggc gtcgcaaacc tgttagccac catgaggctt
     2221 ccggactacg agcaacgggc cacagcagaa gcggcgggag gggcgccatg aaaatttctc
     2281 agctcacccc ccgctgtcgc gcaacgcttc tgggagccgc cgcgatgctg ggcgcacctc
     2341 aactcacatc cgccattcag atgaatgtgt atgtctccac gcccagccag cggggcaccg
     2401 actccgcttt ctatctcggc cacacagtga tggcctccac gcctttcaac gtcctcaatg
     2461 ccgggggcaa ctacacggtg cagtgcaatc acaccgcgac cctgcccatc acgggcgaac
     2521 ggacgttggc cagcacaacc tatgggttcg ataggaacac gctcaccgtc accatcccgg
     2581 cgcagcagcc cgcaatccgg aacctttcgg gatggctcca gattcccgac tacacggctc
     2641 tgagctgcaa ctatcgctgg accgcattcg cgacggaggg tggttattcc attggcgcgg
     2701 gaggcatcag ctttccgatc ggcaacggca ccgtgcgcga cggtgacacc gtcgacttca
     2761 cgatgtaccg cccctccagg gaagacgccg acggaggctg cattccctga tcgctttccc
     2821 tcttcctttc atcaatacca aggatggtag ccatgaaaaa tctctatcga atctttgcca
     2881 ttgctgtttt cgccacggcg tcccaactct gcaacgcctg gggttcatgc acacccatcg
     2941 tcatcgatct cggcaacaac ggcatcaacc tggggcctgc cggtgtcggt gttcatttcg
     3001 atgtgaacgc cgacgggatt cgcgatcatg tgcagtgggt acggcaaggc ggcgatgagg
     3061 gtttcctcgc tttcgaccgc agtgggaatg gcgtcgtcga cgacggcgcg gagctgttcg
     3121 gtgtgggcac gccgctggtg ctcgagggac gcaacgcgcg caacggtttc gtgggtctgg
     3181 cgcagtacga ctcacggcag ttgggaggca acgacgatgg gctcatcacc gaagcagatg
     3241 ccatctggcc gcaactccgg atctggatcg atgcgaacgc ggatggcgtg tccacgcgcg
     3301 acgaaatgcg cacgctgaga agtttcgggc tcacggcgct cgataccatc ccgaaaatac
     3361 gcaagtacgt cgatgccgcg ggcaacgtca ttccctactg ggcgtgggca cagcaacgag
     3421 ctcgtccagg tcgcgcgctc atggtcgacg tattctttgt cgtgttgcct tgaggagccg
     3481 acctctcacg gactcagcat gcgcaccagc gccacgcggg attccacatt gagcttccgg
     3541 aatatctggc gcacttgaga tttgacggta ttcggcgaga cgccgcgcag cctggctacc
     3601 tgcgcgagcg tccgcccttc gcacaggaaa tccgccaccg cttgttcggc ggcggtcaga
     3661 accacggcgc ccggcagatg cagcacacag gtgaatgtga acggggcagc ggagtcggtg
     3721 gcgccctgca ggcgaaacgc gagcccatga gcgcgctctt ccaccgccag cgttctgcaa
     3781 gggccgacca gccgggcaat gagaggcgca gcgtcgggaa tgcgtgcgga ccgccgcagc
     3841 ctcgtgccgc cgcaggagcg cagttcggcc aggacctggg tcaacgtctt ggctgccata
     3901 gtgccgttct gccacgaact tcgcagcagc cgacactcat atatctgccc ggaatctgca
     3961 gatttgcagt caggcggtga atttcttgac ccaggcgacg tacttgtcca tccagccatg
     4021 cacgaacttg cgcgtatcgg cgtttgcaat gttgccggct tcgtcgaaga agccgtcctt
     4081 cacctgaagg tacgcctcgg gctgcccgag cgtgggcatg tcgagatacg caaggatggt
     4141 gcgcaagtgc agctgcgcga cggccgtgct gatggcgcca atcgaggcgc caatgacacc
     4201 ggccggcttc cctgcccacg cgctctggcc gtagggtcgc gaggcatggt cgatcgcatt
     4261 cttgagcacg cccggaatgg agcggttgta ttccgcggtc acgaagatca ccgcatcgac
     4321 cgcacgcagc tcgctcttga gcctctgcac ttccggtgcc tgggacttgt cgtcgtcctg
     4381 gttgtacagc ggcagatcgc cgatgcgcag gtccttgcag gtgaagtccg gcggcgcgag
     4441 cttgatgagc gcattggcca gttttcgatt gatggattcc ttgcggagac ttccgacgac
     4501 gacgccgatc tgcttgggca ttcacgactc ctgtttgcgc gccatcatct caaattcccc
     4561 gtgacgttct gtaggaagag ggcgcgcgtg acgcggtgca tccagtgagg ccggcttttc
     4621 gtagggccta ctgctatgca caaacgcgca ctccagttgc tgttcgcctt cattctggtt
     4681 tccctcactg ccttcagcct ctgggcgagc acgcgccagc cggtctggga gtggggaggc
     4741 ctcacgacac cgcctgacaa tctctggacg atcgccacac tcatcgacgc gtactatggg
     4801 ttcattacct tctacgcgtg ggtcctgtgg aaggaggtgc gcgccctgcc gcgcgtgctg
     4861 tggttcatcg ccatcatgct tctcggcaac atcgcgatgt cggcctacgt gctgcgccag
     4921 ctcgcgcgcc tggaaccggc ggactccatg gccacactgc tctcggcgcg caacgcgtga
     4981 cgcacatgcg cgaattcctg caacgacgga tcgtcgctcc actggtcatg ctcctgcgcc
     5041 agggggtgac gccacgcgag ctcgcgctgt gcctggcgct cggcattacg atcggcctca
     5101 tccccgtgct cggagtgtcg a
//
LOCUS       DASQJS010000228         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4528492, whole genome shotgun sequence.
ACCESSION   DASQJS010000228 DASQJS010000000
VERSION     DASQJS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4528492"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1594)
                     /locus_tag="VEW08_09255"
     CDS             complement(<1..1594)
                     /locus_tag="VEW08_09255"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HYJ40965.1"
                     /translation="MEAPPFAGRPANPFPYENAGRHLKFGLNLFAGFLLPAFTIAFEL
                     ITRMCAEVLFDPLPSLAHLAAVTAVPLINLKLLLMVKREQPVTRGWLFAGAAAAAVGL
                     SYALLFLPVYPFAVIGIIYFGLGLLPFAPLSAGLTALFVTVGAARNAQRPYLRLMICG
                     LLAGFVLVLALDAPTAITRAAVRGSVDADPAVRARSVNLMRNFGNRDLLLRLCYDSSG
                     HTGGLLGLLVEGALSLQLDDFDRRRLTVSPGAARELYYRINGVPYNTVAPPFRGRGWR
                     FADDFAWDADQGGTVIGGRVNGLSLHSSRIDGSMDADDAVAYVEWTTEFTNTSAMQRE
                     ARMTLVLPPGGAVSRATLWVNGEEREAAFATRAAARAAYQAVVMRQRDPLLVTTNGAD
                     QVFVQMFPVPPGGTAKFRIGITAPLTLTNDGRATLALPAIVDRNFSIDAALRHAVWIE
                     GEGSEANADSGFSMIAGENGVVRRRGNFTDQELAVRRPRISIARNPKAEEIASGTVMQ
                     TIRLEAREPARTFYVVLDGSLQA"
     gene            complement(1756..2133)
                     /locus_tag="VEW08_09260"
     CDS             complement(1756..2133)
                     /locus_tag="VEW08_09260"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HYJ40966.1"
                     /translation="MSRYVDGFVLPVPKKNLARYRRVARVAGKVWRDHGALEYVECVA
                     DQLTSKHDGKTFKSLFPKLARVKKGEVVVFSWITYKSKADQKRIMKKVMADPRLSKMM
                     NPASMPFDMSRMAWGGFKPIVEA"
     gene            2283..>2881
                     /locus_tag="VEW08_09265"
     CDS             2283..>2881
                     /locus_tag="VEW08_09265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ40967.1"
                     /translation="MSTAELQTPSTKPGLPRQVPYIIGNEACERFSFYGMRNILTPFL
                     VTSLLLYAPEAERAGIAKDVFHSFVIGVYFFPLLGGWIADRVLGKYRTILWLSLVYCV
                     GHACLAAFDDNRTGFYIGLFLIALGSGGIKPLVVSFVGDQFDQTNKHIAKVVFDAFYW
                     TINFGSFFASLLMPIFLRNYGPAVAFGIPGILMFIATVI"
BASE COUNT          532 a          934 c          880 g          535 t
ORIGIN      
        1 tggcttgcaa cgagccgtcg agcacgacgt agaaagtgcg ggcgggttcg cgcgcctcga
       61 gccggatcgt ctgcatcacg gtgcccgacg cgatctcctc cgccttcggg ttgcgcgcaa
      121 tggaaatgcg cggccggcgg accgcgagct cctgatccgt gaagttgccg cgccgccgga
      181 cgacgccatt ttcgccggcg atcatactga accccgagtc tgcgtttgcc tcgctacctt
      241 cgccctcgat ccacaccgcg tggcgcagcg ctgcatcgat ggaaaaattc cgatcgacga
      301 tagccggcag cgccagcgtc gcacggccgt cattggtcaa cgtgagtggc gcggtgatgc
      361 caatacgaaa cttcgcagtg ccgccaggcg gcacgggaaa catctgcacg aacacctggt
      421 ccgcgccgtt ggtggtgacc agcagcggat cgcgctggcg catgaccacc gcctgatacg
      481 cggcgcgtgc cgcggcgcgc gtggcgaatg cggcctcgcg ttcttcgccg ttcacccaga
      541 gcgtggcgcg cgaaaccgcg ccacccgggg gcaataccag cgtcattcgg gcctcccgct
      601 gcatggcact cgtgttcgtg aactcggtgg tccactccac atacgcaacg gcgtcgtccg
      661 catccatcga gccgtcgatg cgcgagctgt gcaacgaaag cccgttgacg cgtcctccta
      721 tgacagtgcc gccctgatcg gcatcccagg cgaagtcgtc ggcgaatcgc cagccgcgcc
      781 cgcgaaatgg cggcgccacc gtgttgtatg gcactccgtt gatgcggtag tagagttcgc
      841 gcgcggcgcc ggggctgacg gtgaggcgac gccggtcgaa gtcatccagc tgcagactga
      901 gcgcgccttc cactaacaac ccgagcaggc cgccggtgtg gccgctggag tcgtagcaca
      961 ggcgcagcag caggtcgcga ttgccgaaat tgcgcatgag attcacggac cgcgcgcgca
     1021 ccgcgggatc cgcgtccacg ctaccgcgca cggcagcgcg cgtgatcgcg gtgggcgcat
     1081 cgagcgccag caccagcacg aacccggcca ggagtccgca aatcatcagc cgcaggtacg
     1141 ggcgctgcgc gttccgggcg gccccgaccg tcacgaacaa tgcggtgagg cccgcgctca
     1201 acggcgcgaa cggcaacagg cccaagccga agtagatgat tccgatgacc gcgaagggat
     1261 acacgggcag gaacagcagc gcgtatgaca agcctaccgc ggcagcggcg gccccagcga
     1321 acagccagcc gcgcgttacc ggctgctcgc gcttgaccat cagcagtagt ttgagattga
     1381 tgagtggcac cgccgtgacg gcggcgagat gcgcgaggga tggcagcgga tcgaacagga
     1441 cttccgcgca catccgcgtg atgagttcga aggcgatcgt gaaagcgggc agcagaaatc
     1501 cggcaaaaag attgagaccg aatttcaggt ggcgtcccgc gttttcatac gggaacgggt
     1561 tcgcaggccg gccggcgaac ggtggcgctt ccattcatca ctccctttcg ttttctacgg
     1621 ccggagatgc tagacctgga atacggcggc gcgatggatc aattgtggcg aattatttcg
     1681 cgaataggac tttcaacgtc ggctcggacg cgcgatggaa tcggccatca gccgcacctg
     1741 ttttcatcgc gccgcctacg cttcgacaat cggcttgaag ccgccccagg ccattcggct
     1801 catgtcgaaa ggcatgctgg ccggattcat catcttggag aggcgcggat ccgccatcac
     1861 ctttttcatg atgcgtttct ggtcggcctt ggacttgtag gtgatccagg aaaacaccac
     1921 cacctccccc ttcttcaccc tggcgagctt cggaaagagg gatttgaaag tcttgccgtc
     1981 gtgcttgctg gtgagctggt cggcgacgca ctcgacgtac tcgagcgctc cgtggtcgcg
     2041 ccagactttg ccggccacgc gggcgacgcg gcggtagcgc gcgaggttct tcttcgggac
     2101 gggtaggacg aatccatcta cgtatcggga catgggtttc tccttggtag ttacatccag
     2161 tcgagcggcg agcccccatt tcgacacccg ccggaaaaat agttctcgaa gctgagccag
     2221 gcgttgccga atgcctttcg atgtacgctc ctacacccga gtgaaccgca gacccatcat
     2281 cgatgtccac cgccgagctc caaaccccat cgaccaagcc cggactgcca cggcaggtcc
     2341 cgtacatcat cggcaacgag gcgtgcgagc gcttcagctt ctacgggatg cgcaacatcc
     2401 tcacgccgtt cctggtcacc tcgctcctgc tgtacgcacc cgaagccgag cgtgcaggaa
     2461 tcgccaagga tgtattccat tccttcgtga tcggcgtgta cttctttccg ctgctcggcg
     2521 gctggatcgc cgatcgagtg ctcggcaagt accgcacgat cctttggctg tcgctcgtct
     2581 actgcgtggg ccacgcgtgc ctcgccgcat tcgatgacaa ccgcacgggc ttctacatcg
     2641 ggctgttcct gatcgcactg ggctccggcg gcatcaaacc actcgtcgtg tcgttcgtcg
     2701 gagatcaatt cgaccagacg aacaaacaca tcgcgaaggt cgtatttgat gcgttctact
     2761 ggaccatcaa tttcgggtcg ttcttcgcct cgctgctcat gcccatcttc ctgcgaaact
     2821 atgggccggc agtcgccttc ggtatccccg gaatcctgat gttcatcgcc accgtcatct
     2881 t
//
LOCUS       DASQJS010000229         8246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4212816, whole genome shotgun sequence.
ACCESSION   DASQJS010000229 DASQJS010000000
VERSION     DASQJS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4212816"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VEW08_09270"
     CDS             <1..1459
                     /locus_tag="VEW08_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40968.1"
                     /translation="WHETFVAIMRPLLEGPELTFEQALAALRSATGLQLLAFDEQARR
                     GRYYWQPADRFAKDVIRTALGYPEHSDAYLFYEACNGSGFIREQALRALRGRDDRLVC
                     AAILIRIEDWVPQVADVAERLLKDVAATDAARHFFEFLDLITALRSRRRFEPHWVATL
                     EPMLLRSKWRDARRAALSSGDDDVRLLAHQLIRRADEDAAPDTLRIAISDLSPRVALW
                     ALSKVDDSAEPALRQSLLHEGLRSRRASIRREALRRYCRSGYGDLRELLHAAIFDPAH
                     SVRTVAAFQINALFGESALPRWREAFDRGNRGEAMVMALSESGDAQDDARLRTQLRAS
                     RGRIRALALRGLLRIGAADSAKLLDAAMRDSSVFVVGAAIAGYAFGTEQLAAATLHEA
                     FMLAKAPAMRARLISASRLLPKWRRLQFLLELCASCGEADREPLNAAVATWIASANRT
                     FTEALPEQKAALRSALETARSTHPARFWDHVQRLI"
     gene            1597..2448
                     /locus_tag="VEW08_09275"
     CDS             1597..2448
                     /locus_tag="VEW08_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ40969.1"
                     /translation="MGTNNIVLIHGLWMTPLSWEFWAHHYTERGYSVYAPSWPGMERD
                     VRALRRAPETYADLGFKRIVDHYEKLVIDLESAPIIIGHSFGGLVVQALLDRGLGACG
                     VAIASAPIKGIWTLPYSTMRVVTPQLINPMNNRRCVPLTPAQFHYAFMNTSTREESFH
                     VYQRYAVPGPDHILFQTELANFNPFAETQVNVRRNNRAPLLMIAGGSDHLIPPSIIKA
                     NVKAYRHSTAVTNYKEFPQRTHFIIGQPGWQEVANYSIDWSLDQQLLIDRERRRVARE
                     VQARQVA"
     gene            complement(2528..3211)
                     /locus_tag="VEW08_09280"
     CDS             complement(2528..3211)
                     /locus_tag="VEW08_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HYJ40970.1"
                     /translation="MNRLHEAIRRLPPGVEFLVVVTWAFGLPIFASIMSLAAPNEGSS
                     SVFTNVELFAAIIFEVIQLGFLAWFLRIRGWTLEKFGLAFSWSGTAVGLALLAVTYAL
                     VVGVQYFAQVVFDYDMQAAIARAPKFDEDLSMQLLFLISVVNAIAEELFVAGYVITAL
                     QPRRGMWVAINMSIVVRLLYHLYQGPIGVLTAVPMGLLYGYLYSRTRQLWPLIVAHVV
                     LVLISLSTR"
     gene            complement(3218..5170)
                     /locus_tag="VEW08_09285"
     CDS             complement(3218..5170)
                     /locus_tag="VEW08_09285"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ40971.1"
                     /translation="MSIIDRILGRTPTEGPQIHASRTSTPAKPARITGLPPAPVAAAP
                     EPAATSASEGMIRVYDQFGRAVTIGREAWRRDVLLPNLQSNRNSPDALYDLVVSALND
                     GFATDVLDSARHLAENDTQPHRGAMVLGIVLLQLKDHVAARDVLERAIARYGENPYLL
                     ANLARAYAAAGDEARAQALIWRALELEPNEETALNWMIGMARPQGQDAVLSAYTRAAA
                     LPGSWRALLWLARYALDRGDLAEATRLYELALARATPTPADLLMQLSGDLGNRGHTEL
                     LMKLTQPRFDLLEHGLTVGNNLLRAYVELGMFAEARKLLEQLYSQQRPDWREQLIAWE
                     QKLDDAEKRYGEVTAPVEVVVMKLEQPVWARGVLGFDAVLPAKTHAAPRIHFICGSGE
                     ANEEMGGKVVAQPTNDLGRIARALPMFLAEELYLRTNARTAFLLPWMKQGGFILSAKP
                     WTRAFLPSDHTPPDLIVYLHVDARVSPWLLKVTIENAQREVTPVVFEQAFTLQTAGQD
                     VLTLLYDLIPRLTLLLALRREESNSALGTPPPELLPGYLAAIEQALAIGLAARHAGGG
                     SFLHQERSIFDHLFDVALQSDQLLRPRMLLVNALENQTRRRPDIAREYLEKLAMLQQR
                     HAQTSSVGNELVAKGVEKVSEKAKAG"
     gene            5245..6930
                     /locus_tag="VEW08_09290"
     CDS             5245..6930
                     /locus_tag="VEW08_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HYJ40972.1"
                     /translation="MSFAHAEDVLIKTRDGVTLSATMVLPEPRPGGRISTILEFTIYS
                     EPAKLKAAAEEIAARGYAGIVADARGKRLSPDAPAPYETEALDTHAVLDWIVEQPWSD
                     GNVGMIGGSYTGFTAWAATKRRHPALKGIAVSAAAIPGLGLPMYNNVFLNANYQWAFY
                     VTRNKLVDDAVNDDGQRWWKMQREWFLSGRPYREIDAVDGTPNPWLQRWLQHPAYDKY
                     WQSMVPYEGDFARINIPVLSITGYYDDGQISALEYMKQHLAHARNPEHYLVIGPYDHF
                     GASARTKPEVLREYTIDPVAQLDSQELKLAFMDYVLKGKPKPALLKDKINYEVMGANL
                     WRHSPSLGSVHGGVPMRLYFSSQKSEGLYSLANRQPPSDSLVTHEVDFADRVKFHNFH
                     SYPMPIVQGPLSYVTESMFLSEPFEATAVISGSFTGELTVVVNKKDFDLGVTVYEAMQ
                     GGKLFHLGYAIHRASYGEDRTKRHLLSPGKTEHVKFETTVVSRQMQPGSRLLVLVDAI
                     KNPVAQVNYGTGKDVSDESIKDAGDPLKIEIRSGSYFQVPLDNSFSTKPVSPQ"
     gene            complement(7017..7373)
                     /gene="yecR"
                     /locus_tag="VEW08_09295"
     CDS             complement(7017..7373)
                     /gene="yecR"
                     /locus_tag="VEW08_09295"
                     /inference="COORDINATES: protein motif:HMM:NF025358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR family lipoprotein"
                     /protein_id="HYJ40973.1"
                     /translation="MTQFTKSLRFLASAALLLNLAACATSQNWGISGGDRNGGVVRVS
                     YEYPEFHQPDVNDAQAMKIALSRCSGWGYDKVEALPGQTRQCSNMEGANCDLWTVTRE
                     YQCTTADGALASNLAQ"
     gene            complement(7451..7801)
                     /locus_tag="VEW08_09300"
     CDS             complement(7451..7801)
                     /locus_tag="VEW08_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYJ40974.1"
                     /translation="MVTRKKRSSKRDHETGSGNVYADLALADPEAMLVKAKLVTRIAE
                     LLASRDFTQTEGAALLGIPQPKLSNLLNGRFRGFSERKLMDFLTRLGSDVEIVVRSGT
                     RAGRRGSVSVVIAS"
     gene            complement(7795..8181)
                     /locus_tag="VEW08_09305"
     CDS             complement(7795..8181)
                     /locus_tag="VEW08_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYJ40975.1"
                     /translation="MGILESAPKTSIPKPVVWVGSSRKDLITLPTTVVDTFGYGIYLA
                     QIGRRHEKSKTLRGFGDAGVIELIDSRAANTFRAVYTVRFPVAVFVLHVFQKKAKSGS
                     STPKQDIELIAVRLRRAAELAKEMKW"
BASE COUNT         1605 a         2608 c         2518 g         1515 t
ORIGIN      
        1 ttggcacgag actttcgtcg caatcatgcg accgctcttg gaagggccag aactcacttt
       61 cgagcaggcg ctcgcggcac ttcgcagtgc gaccggattg cagctgctgg ctttcgacga
      121 gcaagcccgc cgcggccgct attactggca gccggcggac cgattcgcga aggacgtaat
      181 caggaccgcg ctcggctatc cggaacattc tgacgcctat ctcttctacg aggcgtgcaa
      241 cggaagcggt ttcatccgcg agcaggcgtt gcgcgccttg cggggacgcg acgatcgatt
      301 ggtttgtgca gccatcctga tacgcatcga agactgggtc ccacaggtgg ccgatgtggc
      361 ggagaggttg ttgaaggatg tcgcggcgac cgacgctgcg cgccacttct tcgaattcct
      421 cgacctgatc acggccttgc gatcgcgccg tcgcttcgaa ccacactggg tggcgaccct
      481 cgaacccatg ttgctgcggt cgaagtggcg cgacgcgagg cgcgcggcac tgtcgagtgg
      541 cgatgacgat gtgcgactcc tggcgcatca actgatccgg cgcgctgacg aggacgccgc
      601 acccgacacg ctgcgcattg ccatcagcga cctctcgcct cgcgtagcac tgtgggcact
      661 gagtaaagtt gacgattcgg ccgagccggc tcttcgccag tcattgttgc acgaaggatt
      721 gcgcagccgg cgggcctcga tccgccgcga ggcgttgcgt cgctattgcc gctccggcta
      781 cggcgacctg cgtgagttgc tacatgccgc cattttcgat cccgcgcatt ccgtgcgcac
      841 tgttgccgct tttcagatca acgcactgtt cggtgaatct gcgctgccgc gatggcgcga
      901 ggcctttgac cgcggcaatc gtggcgaggc catggtgatg gcgctttcgg aatccggcga
      961 tgcccaggac gatgcgcgcc tgcgtacgca acttcgagcc agccgcgggc gaattcgcgc
     1021 attggccctg cgaggactgc tgcgaatagg cgcggcagac agcgccaaac tactggatgc
     1081 ggcgatgcgc gactcgtccg tgttcgtcgt cggcgcggcc attgccggat acgcgtttgg
     1141 cacagaacag ctcgctgccg cgacgctgca cgaagcgttc atgctcgcga aggctccggc
     1201 gatgcgcgcc cggctcatca gcgcatcgcg gctcctgccg aaatggaggc gcttgcagtt
     1261 tctgctggaa ctttgcgcca gctgcggcga agcggatcgc gagccgctga atgctgcggt
     1321 ggcgacctgg atcgccagtg ccaatcgaac ctttaccgaa gctcttccgg aacagaaggc
     1381 cgctcttcgc agcgcactcg aaaccgcgcg aagcacgcat cccgcccggt tctgggatca
     1441 cgtgcagcgc ttgatataga aatttcgcaa cgtacgtcaa aaatccgacg ccttggaatt
     1501 cgccggaccc gaaaatcccc tacgtacaaa ttctccctgg tgtcgctgga caacgacaac
     1561 gcctcgggag aatctgtcgg ctcatgtgga ggcctaatgg gcactaacaa catcgtcttg
     1621 atccacgggt tatggatgac gccgctcagt tgggagtttt gggcgcacca ctacacggaa
     1681 cgtggataca gcgtgtatgc gccaagctgg cccggaatgg aacgtgacgt tcgagccctg
     1741 cgtcgcgccc cggaaacgta cgcggatctc ggtttcaagc ggatcgtcga tcattacgaa
     1801 aagctcgtca tcgatctcga aagcgcgcca atcatcatcg ggcattcgtt tggcggactc
     1861 gtggttcagg cattgttaga ccgtgggctg ggagcctgcg gcgtggccat agcatcagcc
     1921 cccatcaagg gaatctggac acttccctat tcaacgatgc gagtggtcac gccgcagctt
     1981 atcaacccca tgaacaaccg ccgttgcgtg ccactcacgc cggcgcagtt ccactacgcc
     2041 ttcatgaaca cttccacgcg cgaggaatcc tttcacgtct accagcgata tgcggtgccc
     2101 ggtcccgacc acatcctgtt ccagaccgag ctcgccaact tcaatccgtt tgccgaaacg
     2161 caggtcaacg tgaggcgcaa caatcgcgcg ccgctgctga tgatcgccgg cggtagcgat
     2221 cacctgatcc cgccgagcat catcaaggcc aacgtgaagg cctaccggca ttccaccgcg
     2281 gtcaccaact acaaggaatt tccgcagcgc acgcacttca tcatcggcca gcccggctgg
     2341 caggaagtgg ccaactactc gatcgactgg tcgctcgacc agcagctgct catcgatcgc
     2401 gaacggcggc gagtggcgcg cgaagtgcag gcccgccagg tcgcctaaat ggtgaaatgg
     2461 tgtcaggcac cgtttcctag gaaatggtgc caggcaccga ttcctggaaa acggtgcctg
     2521 acaccattta acgcgtcgac aggctgatca gcaccagcac cacatgcgcc acgatcaagg
     2581 gccatagctg gcgcgtgcgt gaatacaggt agccgtacag cagccccatc ggcaccgcgg
     2641 tcagcacgcc gatcggcccc tggtacaggt gatacagcag ccggaccacg atgctcatgt
     2701 tgatcgccac ccacatcccc cggcgcggct ggagcgcggt gatgacgtac ccggccacga
     2761 acagctcttc ggctatcgca ttgaccaccg agatcaggaa cagcagctgc atgctcaggt
     2821 cttcgtcgaa cttgggcgcg cgcgcgatgg cggcctgcat gtcgtagtcg aagaccacct
     2881 gcgcgaaata ctgcacgccc acgacgagcg cataagtcac ggcgagcaac gccaggccaa
     2941 ccgccgtacc gctccaggag aacgccaggc caaacttctc cagtgtccat ccacgaatgc
     3001 gcaggaacca ggcgaggaac ccgagctgga tcacttcgaa aatgatggcg gcgaacagtt
     3061 cgacgttggt gaagaccgac gaactgcctt cgttcggcgc cgcgagcgac atgatcgacg
     3121 cgaagatcgg caggccgaac gcccaggtga cgacgaccag gaattccacg cccggcggca
     3181 ggcggcggat ggcttcgtgc agtcgattca ccgcgactca acccgccttc gctttctcgc
     3241 tcactttctc cacacccttc gccaccagct cgtttcccac gctcgaggtc tgcgcgtgcc
     3301 gctgctgcag catcgccagc ttttccaggt attcgcgcgc gatgtccggc cggcggcgcg
     3361 tctgattctc gagcgcgttg acgagcagca tgcgcggcct gagcaactgg tcgctttgca
     3421 gcgccacgtc gaacagatga tcgaagatcg agcgttcctg gtgaaggaac gatcctcccc
     3481 ctgcgtggcg cgcggcaagg ccgatggcca gcgcctgctc gatggcggcg agataacccg
     3541 gcagcaactc cggcggcggc gtgccgagcg ccgagttcga ttcctcgcgt cgcagcgcca
     3601 gcagcagggt gaggcgcgga atcaggtcgt acagcagcgt gagaacgtcc tggccggctg
     3661 tctgcaacgt gaacgcctgc tcaaacacca cgggagtgac ttcgcgctgc gcgttttcga
     3721 tcgtcacctt cagcagccag ggcgacaccc gcgcatccac gtgcagatag acgatgagat
     3781 ccggcggcgt gtgatccgat ggcagaaacg cgcgggtcca gggcttggca gagaggatga
     3841 aaccgccctg cttcatccac ggcaacagaa atgcggtgcg cgcattggtg cgcaggtaga
     3901 gctcttcggc cagaaacatg ggcagcgccc gcgcgatgcg tccgagatcg ttggtcggtt
     3961 gcgcgacaac cttgccgccc atctcttcgt tggcttcgcc gctgccgcag atgaaatgga
     4021 tgcgtggcgc ggcgtgggtc ttcgccggca gcacggcatc gaaaccgagt acgccgcgag
     4081 cccaaacggg ttgctcgagc ttcatgacca ccacctccac cggcgcggtc acttcgccat
     4141 atcgcttttc ggcgtcgtcg agtttctgct cccaggcgat gagctgctcg cgccagtccg
     4201 ggcgctgttg cgaatacaac tgctcgagca gtttgcgcgc ctcggcaaac atgccgagct
     4261 ccacgtaggc gcgcagcagg ttgttaccca ccgtgagccc atgctcgaga aggtcgaagc
     4321 gcggttgagt cagcttcatc agcagttcgg tgtggccgcg atttccgagg tccccgctca
     4381 gctgcatgag caggtccgcg ggggtcgggg tggcgcgcgc cagggccaat tcgtacagcc
     4441 gcgtcgcctc ggcaagatcg ccgcgatcga gcgcataacg cgcgagccac agcaatgcac
     4501 gccagcttcc cggcagcgcc gccgcacgcg tgtacgcgga aagcaccgcg tcctgcccct
     4561 ggggacgcgc catgccgatc atccagttga gcgcggtctc ttcgttcggc tcgagctcga
     4621 gcgcgcgcca gatgagtgcc tgggcacggg cttcatcgcc ggctgccgca taggcacgcg
     4681 cgaggttcgc cagtagatag ggattttcgc cgtatcgcgc gatggcgcgt tccagcacgt
     4741 cgcgcgccgc cacgtgatcc ttgagctgca gcagcacgat ccccagcacc atggcgccac
     4801 gatgcggctg ggtgtcgttt tcggcgaggt gccgcgcgga gtcgagcacg tccgtggcga
     4861 agccgtcgtt cagcgcgctc accaccaggt cgtagagtgc gtccggacta ttgcgatttg
     4921 actgcaggtt cggcagcagc acgtcgcgcc gccaggcctc gcggccgatg gtcacggccc
     4981 ggccaaactg gtcgtaaacc cggatcatgc cctcgctggc cgaggtggcc gccggctccg
     5041 gggcggccgc cacgggtgcg ggcggcaacc cggtgatgcg cgctggcttg gccggcgtgg
     5101 acgtccgcga agcatgaatc tgcggtccct cggtgggcgt gcggccaagg atgcggtcga
     5161 tgatggacat ggggcggtga gcttacactt gtgggcatgc gccgcacctt actgccatgc
     5221 ctttccgccc tcctgctggg ggttatgtcg ttcgcacacg ccgaggacgt gctcatcaag
     5281 acgcgtgacg gcgtcacgct ctcggccacg atggtgctgc ccgaacccag acccgggggc
     5341 cgcatatcga ccatcctgga attcaccatc tactctgagc ctgcgaagct gaaggccgca
     5401 gccgaagaaa tcgcggcgcg cgggtacgcc ggcatcgtgg ccgacgcgcg cggcaagcga
     5461 ctgagccccg atgcgcccgc gccctatgag actgaggcgc tggacacaca cgccgtgctc
     5521 gactggatcg tcgagcagcc ctggagcgac ggcaacgtgg gcatgatcgg cgggagctat
     5581 acgggcttca ccgcctgggc ggccaccaag cgcaggcatc ccgcgctcaa gggcatcgcg
     5641 gtgtcggcgg ccgcgatccc cggcctgggg ctgccgatgt acaacaacgt gttcctgaac
     5701 gctaactatc agtgggcgtt ctacgtcacc aggaacaagc tggtggacga cgcggtgaac
     5761 gacgatgggc agcgctggtg gaagatgcag cgcgaatggt tcctgagcgg gcgcccgtat
     5821 cgcgaaatcg acgccgtcga cggcacaccg aacccgtggc tgcaacgctg gctccagcat
     5881 ccggcctacg acaagtactg gcagtccatg gtcccgtacg agggtgactt cgcgcgcatc
     5941 aatatcccgg tgctcagcat taccggctac tacgacgacg ggcagatctc cgcgctcgag
     6001 tacatgaagc agcatctcgc ccacgctcga aacccggagc actacctggt catcggcccc
     6061 tacgatcact tcggcgcgag cgcgcgcacg aagccggaag tgctgcgcga gtacaccatc
     6121 gacccggtgg cgcagctcga ttcgcaggaa ttgaaactcg cgttcatgga ctacgtgctc
     6181 aagggcaagc cgaagccggc actgctcaag gacaagatca actacgaagt gatgggagcg
     6241 aacctgtggc ggcactcgcc gtcactgggc tccgtgcatg gcggcgttcc catgcgcttg
     6301 tatttctcgt cgcagaagag cgaaggcctg tattcgctcg ccaaccggca accgccctcg
     6361 gattcgctgg tgacgcatga ggtggatttt gccgaccgag tgaaatttca taactttcat
     6421 tcgtatccca tgcccatcgt gcaggggcca ctctcctacg tcaccgaaag catgttcctg
     6481 agcgagcctt tcgaagcgac cgccgtgatc agcggttcgt tcacgggcga actgacggtg
     6541 gtggtcaaca agaaggactt cgatctgggt gtcacggtgt atgaggcgat gcagggcgga
     6601 aagctgttcc acctgggcta tgccatccat cgcgcgagtt acggcgagga ccgaacgaag
     6661 cggcacctgc tctcgccggg caagacggaa cacgtgaagt tcgaaaccac cgtggtgagc
     6721 cggcagatgc agcctggctc gaggttgttg gtactggtgg atgcaatcaa gaatccggtg
     6781 gcgcaggtga actacggcac cggcaaggac gtgagcgacg agtcgatcaa ggatgctggc
     6841 gatcctctga agatcgaaat acgcagcggc agctacttcc aggtgccgct cgacaactct
     6901 ttctcgacaa agccggtcag ccctcagtaa gtaaaagggg acagatctat taattgaatg
     6961 cagccacagg ttcgcagtgg acagtcgggt caataaatag atctgtcccc ttttacttac
     7021 tgcgcgagat tgctggcaag cgctccatcc gcggtggtgc actggtactc gcgagtgacg
     7081 gtccacagat cgcagttcgc gccttccatg ttgctgcact ggcgggtctg cccgggaagc
     7141 gcttcgacct tgtcatagcc ccaacctgag cagcggctta acgcaatttt catggcctgc
     7201 gcgtcgttta cgtcgggctg atggaactca ggatattcat aggacacccg gaccacaccg
     7261 ccgttgcggt cgccgcccga aataccccag ttctgactgg tagcgcaggc ggcgaggttc
     7321 agcagcagcg ctgcggaggc gaggaaccgg agtgatttcg tgaactgggt catgggcgcg
     7381 gtccaggact tatcttgaat cccagattgc gcccccggtc cttaaccgat cctgaatccc
     7441 actcaaaaag tcagctggca atcaccacgg acaccgaacc gcggcggcca gcccgtgtac
     7501 cacttcgcac cacgatttcc acgtcgctgc caagtcgcgt caggaaatcc atgagcttgc
     7561 gctctgagaa cccccgaaat cgcccgttga gcaagttgga aagcttcggc tgcggaatgc
     7621 caagcaacgc tgcaccctca gtttgcgtga agtcgcgcga cgccagaagt tctgcaattc
     7681 ttgtcaccag tttcgccttg acgagcatcg cctcaggatc ggcgagtgcg agatccgcat
     7741 acacattgcc gctgccagtt tcatggtccc gtttcgagct acgcttcttg cgtgtcacca
     7801 tttcatttcc ttcgcgagtt ccgctgcacg ccgcaaccgc accgcaatca gttcgatgtc
     7861 ttgctttggc gtcgaagatc cacttttcgc cttcttctga aagacatgca agacgaatac
     7921 cgcgactgga aatcgaaccg tataaacagc acgaaaggtg ttcgccgcgc gcgagtcgat
     7981 gagctcgatc acacccgcat cgccgaagcc gcgcaatgtc ttgctctttt cgtgacgtct
     8041 accgatctga gccaggtata tcccatatcc gaacgtatcc acgacggtcg ttggcaaagt
     8101 gatcaagtcc ttccggctgc tgccaaccca aacaacaggc ttcgggatag aagtctttgg
     8161 agcagattcg agtataccca tttggatatg gcctgcaatt cggaggatgc ccaaggaaag
     8221 gcatcacgat gtccgatcgc agcggg
//
LOCUS       DASQJS010000230         3851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3586117, whole genome shotgun sequence.
ACCESSION   DASQJS010000230 DASQJS010000000
VERSION     DASQJS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3586117"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1206)
                     /gene="hisC"
                     /locus_tag="VEW08_09310"
     CDS             complement(103..1206)
                     /gene="hisC"
                     /locus_tag="VEW08_09310"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HYJ40976.1"
                     /translation="MKNPGDLALDCVRTLAPYVPGKPIEELQRELGLEDIIKLASNEN
                     PFGASPGALAAMQRAVADTWLYPDGSGHVLKQKLAAKLGVDSTQITLGNGSNDLLVML
                     AEAFLGPGLEAVYSQYAFAVYPIAVQATGAKGVVTPALAPDSPMPLGHDLNAMARAIT
                     PRTRVVFIANPNNPTGTWVPAADLHAFIAATPAHVLIALDEAYFEYTEGLGLQDGINW
                     LREFPNLVVFRTFSKAYGLAGVRVGYAVSHPSVAGMLNRVRQAFNVSVIGLAGASAAL
                     DDTHHLASAVKVAVTERARVTVRLEKAGTRVLPSAGNFLMVHVGEAQKRFEALLRRGI
                     IVRPVGNYGLPEHLRVTLGTVDQNDRFLKAWEA"
     gene            complement(1203..2336)
                     /gene="pheA"
                     /locus_tag="VEW08_09315"
     CDS             complement(1203..2336)
                     /gene="pheA"
                     /locus_tag="VEW08_09315"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017816350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HYJ40977.1"
                     /translation="MAARKKSTGKAAGKAKVADPLAGLRAQIDSVDAKIHALLNDRAA
                     LARQAGVSKHADGHTVDYYRPEREAQVLRMARERNKGPLRTEEILRLFREIMSACLAQ
                     EEPLKVGYLGPEGTFSQTATLKHFGHSVRALALASVNEVFHEVEAGLADFGVVPVENS
                     TEGAVNHTLDLLLGSPLRICGEVELRIHQFLMGGMDNLAKIKRICSHPQSLAQCRGWL
                     DEHLPDVPRIPVSSNAEAARRARDEDGTAAIAGETASDVYGLKLLATEIEDSDDNTTR
                     FFVVGRKLFKPSGDDRTTILCSALDTESPGALFRLLEPLAKGKISLTRIESRPSRRKK
                     WDYVFFFDIVGHAEEVHVAKALTALKKRASLFRVLGSYPRAVS"
     gene            complement(2346..3515)
                     /locus_tag="VEW08_09320"
     CDS             complement(2346..3515)
                     /locus_tag="VEW08_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HYJ40978.1"
                     /translation="MRFKIQTLNNISIKGLERLPRDRFEIASEIGNPDAILVRSADMH
                     KVALPDSLRAVGRAGAGTNNIPVEALSRKGIPVFNAPGANANAVKELVLAGMFLAARN
                     ICQAWAFAKSQQGDDHAIDLAVEKGKKNFVGFELPGRTLGVIGLGAIGVEVANSALGL
                     GMKVLGYDPQITVQRAWQLSSSVEQALSLDDLFVRSDVISVHVPLTPETRGLVNEARL
                     KLMRKNGVILNFARAPIVDEKAIIAALDDARLQGYVCDFPTNALKDHARVVTLPHLGA
                     STNEAEDNCAVMVADTVKDYLENGNIKNSVNFPEVVLPRAPKTARICIANSNVPNMVG
                     QISTVLAGASLNIAELLNKSRGEYAYTLIDTDGAVDAGVIDKVRAIGGVLAARVI"
     gene            complement(3609..>3851)
                     /locus_tag="VEW08_09325"
     CDS             complement(3609..>3851)
                     /locus_tag="VEW08_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     aminotransferase"
                     /protein_id="HYJ40979.1"
                     /translation="SGFYKSPVAPNARSWMNVPFTLAKPDLDKAFCAEAAKAGLVNLE
                     GHRSVGGMRASIYNAMSIEGIEALVNFMKDFQSKNA"
BASE COUNT          634 a         1201 c         1276 g          740 t
ORIGIN      
        1 ggcgacgatg cggctgatgg tgcctttgcc ggagctcgag ggaccatcga tggtgacgat
       61 ggggacagaa gttgtttcag ggctcattgg cccgagaggc tatcaagctt cccaggcttt
      121 caggaaacga tcgttctgat cgacggtacc cagggtcacg cgcaagtgct cgggcaatcc
      181 atagttgcca acgggccgca cgatgatgcc gcgacgaagc aatgcctcga agcgtttttg
      241 agcttcgccc acgtgcacca tcaaaaaatt gcccgccgac ggcaagacac gtgtgcccgc
      301 cttttcgagc cggaccgtca ctcgtgcgcg ctcggtcact gccaccttca ccgccgaggc
      361 aaggtggtgc gtatcgtcca gcgctgccga cgcaccggcg aggccgatca ccgacacgtt
      421 gaacgcctgg cgcacgcgat tgagcatgcc ggcgacggac ggatggctca cggcgtaacc
      481 gacgcgtacg ccggcgagac cgtaggcctt cgagaacgtg cggaacacca cgaggttcgg
      541 gaattcgcgc agccagttga tgccgtcctg caggccgaga ccctcggtgt attcgaaata
      601 cgcctcgtcg agcgcaatga gcacatgagc cggcgtggcg gcgatgaatg catgcaggtc
      661 cgccgccggc acccacgtgc ccgtggggtt gttagggttg gcgatgaata cgacacgggt
      721 gcgtggcgtg atggcgcgcg ccatcgcgtt caggtcatgg cccagcggca tgggtgagtc
      781 cggcgccaat gccggtgtca cgacaccttt tgcgcccgtg gcttgcaccg cgatcgggta
      841 caccgcgaac gcgtattgcg agtagacggc ttcgaggccc ggcccgagga acgcctcggc
      901 cagcatcacg agcaggtcgt tcgaaccatt gccgagcgtg atttgcgtgc tgtcgacacc
      961 cagtttcgcg gccagcttct gcttgagaac gtggccgctg ccgtcgggat agagccaggt
     1021 atcggcaacc gcgcgctgca tggcggccag cgcacccggg ctcgcgccga acggattctc
     1081 gttcgaggcc agcttgatga tgtcctcgag cccgagctca cgctgcagtt cttcgatggg
     1141 cttgcccggc acgtagggcg cgagcgtgcg cacgcagtcg agcgcgaggt ctccagggtt
     1201 cttcacgaca cggcgcgtgg gtacgagcct aggacccgga atagtgaagc gcgctttttc
     1261 agcgcggtga gcgccttggc cacgtggacc tcttcggcgt gccccacgat gtcgaagaag
     1321 aacacgtagt cccacttctt gcgacgcgac gggcgcgact cgatgcgcgt gaggctgatc
     1381 ttgcccttgg ccagcggttc gagcaggcgg aacaacgcgc ccggcgattc ggtgtcgagc
     1441 gccgaacaca ggatggtggt gcggtcgtcc ccggaaggct tgaacagctt ccgccccact
     1501 acaaaaaagc gcgtggtgtt gtcgtcgctg tcttcgattt cggtggccag cagtttcagg
     1561 ccgtaaacat ccgatgcggt ttcgccggcg atggcggcgg tgccgtcctc gtctcgcgcg
     1621 cggcgcgcgg cttcggcatt gctggagacc gggatgcgcg gcacgtcggg caggtgttca
     1681 tcgagccagc cgcggcattg cgccagcgac tgcggatggc tgcagatgcg cttgatcttg
     1741 gccaggttgt ccatgccgcc catgaggaac tggtggatgc gcagttccac ttcgccgcag
     1801 atccgaagcg gcgagcctaa caagaggtcc agcgtgtgat tgacggcgcc ttccgtggaa
     1861 ttctccaccg gtacgacgcc aaaatcggcc agacccgcct ccacctcgtg gaacacttcg
     1921 ttgaccgagg cgagcgccag tgcgcgcacc gagtggccga aatgcttgag cgtggcggtc
     1981 tgtgagaacg tgccttcggg cccgagataa cccaccttga ggggctcttc ctgcgcgagg
     2041 caggcggaca tgatttcgcg aaacaggcgc agaatttctt cggtacgcag cggacccttg
     2101 ttccgctctc gcgccatgcg cagcacctgt gcttcgcgtt ccggccggta gtaatcgacc
     2161 gtgtgcccat cggcgtgctt ggagacgccg gcctggcgcg ccagggccgc gcggtcgttg
     2221 agcagcgcgt ggatcttcgc gtcgacgctg tcgatctgcg cgcgcagacc ggcgagcggg
     2281 tcggcgacct tcgccttgcc cgccgccttg cctgtgcttt tcttcctggc agccatcgcc
     2341 cgctgttata tcacccgggc agccagcacg ccgccgatcg cgcgcacctt gtcgatcacg
     2401 ccggcatcca ccgcgccgtc ggtatcgatg agcgtgtagg catattcgcc gcgggacttg
     2461 ttgagcagct cggcgatgtt cagcgatgcg ccggccagca ccgtcgagat ctggcccacc
     2521 atgttaggca cgttgctgtt ggcgatgcag atgcgcgcgg tctttggcgc gcgcggcagc
     2581 accacttccg ggaagttgac gctgttcttg atgttgccgt tctcgaggta gtccttgacc
     2641 gtgtcggcca ccatgaccgc gcagttgtcc tcggcttcgt tggtcgaagc gccgaggtgc
     2701 ggcagcgtga cgaccctggc gtgatccttc agcgcgttgg tcgggaagtc gcagacataa
     2761 ccctgcaggc gcgcgtcgtc gagcgcagca atgatggctt tttcgtcgac gatgggcgca
     2821 cgcgcgaagt tgaggatgac gccgttcttg cgcatgagct tcaggcgcgc ttcgttgacg
     2881 aggccgcgcg tctccggcgt cagcgggacg tgcaccgaga tgacatccga acgcacgaac
     2941 agatcgtcca gcgacagcgc ctgctccacg ctggacgaca gctgccaggc gcgttgcacg
     3001 gtgatttgcg ggtcgtagcc cagcactttc atgccgaggc cgagcgccga gttcgcaact
     3061 tcgacgccga tggcgcccag gccgatgacg cccagcgtcc ggccgggcag ttcgaagccg
     3121 acgaagttct tcttgccctt ctcgaccgcg aggtcgatgg cgtgatcgtc accctgctgc
     3181 gacttggcga aggcccaggc ctggcagata ttgcgcgccg ccaggaacat gccggcgagc
     3241 accagctctt tcaccgcatt ggcgttggcg ccgggagcgt tgaacaccgg gatgcccttc
     3301 ctggacaggg cctcgaccgg aatgttgttg gtgccggctc cggcgcggcc cactgcgcgc
     3361 aggctgtcgg gcaaggccac tttgtgcatg tcggccgagc gcaccaggat ggcgtcggga
     3421 ttgccgattt ccgaggcgat ttcgaagcgg tcgcgcggca gtcgctcgag gcccttgatg
     3481 ctgatgttgt tgagagtctg gatcttgaat cgcatggtct atctcctcga gcgctgacgt
     3541 tattgccagc ctcttttcac gttacccccg gacgttattg cccggggcgg ttaggtgggg
     3601 cacctgattc aggcgttctt gctttggaaa tccttcatga agttcacgag cgcctcgatg
     3661 ccttcgatgg acatggcgtt gtagatggag gcgcgcatgc cgcccacgga acgatgccct
     3721 tcgagattca cgagccccgc tttcgcggcc tcggcgcaga acgccttgtc gagatctggc
     3781 ttggcgagcg tgaacggcac gttcatccag gaacgcgcgt tcggcgccac ggggctcttg
     3841 tagaagccac t
//
LOCUS       DASQJS010000231         4035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4213027, whole genome shotgun sequence.
ACCESSION   DASQJS010000231 DASQJS010000000
VERSION     DASQJS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4213027"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /gene="mutL"
                     /locus_tag="VEW08_09330"
     CDS             complement(<1..1563)
                     /gene="mutL"
                     /locus_tag="VEW08_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HYJ40980.1"
                     /translation="MPIRVLDNHLIDQIAAGEVIERPASIVKELVENSLDAGAQSVEV
                     EIEAGGVRLTRVRDDGCGIPVAELKLAMSRHATSKIANADDLGAIMTLGFRGEALPSI
                     ASVSRFEITSRHVDEDRAASLTVDAGQVSDPAPAAHPTGTTIEVRDLFYNLPARRKFL
                     RSEVTEQGHIVRLLERLALSRDDVAFRLRSGSRTLLDAPALTATGGAAQRLARIVGSE
                     FVSRAIELDHSAGPVRISGWIGAPSAARATSDMQFWFVNGRAVRDRLLMNAVRLGYRD
                     VLYSGRQPAYVLYLDIDPSLVDVNAHPQKLEVRFRDSRQIHDFVFRAVERKLAGTRPG
                     TSTVPPAYASGSQYTQPMSLHSADGAAPQGVWAVADALRGMSADPHPHEVEGVPSDVR
                     GEQPLGTALAQVHGIYILAQNAQGMVLVDMHAAHERVLYEKLKVQAGASVPTQSLLVP
                     VTVEMKVDELDALMTQSEEWRAAGFDVERLAPETLVVRSVPAMLPTDDIPALVREVIG
                     DVTADSASHHLDS"
     gene            complement(1655..2860)
                     /locus_tag="VEW08_09335"
     CDS             complement(1655..2860)
                     /locus_tag="VEW08_09335"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HYJ40981.1"
                     /translation="MALLAFFAGVSGNLVAAEIREVRIASTETGTRVVLDLSAPAKHK
                     AFLLDSPHRVVVDLSKSSLLSKLPDAEGVVTSMRSGELPHSGLRLVFEVSGPVTLQTS
                     AAMPSGDAGHRLILDIAGPAASGVAKTVAVTPTVPAAPVAIRPAHAPSDSGRDIVIAI
                     DAGHGGVDPGASGKRGTREKNVVLEIAKALAARINAEPGMKAVLTRDGDYFISLKERT
                     LRARRAKADLFVSIHADAIANPDVSGSSVYVLSDRGASSEAARWLAERENAADLKGGI
                     KLDDKDAVLANVLLDLSQTASISASMVAAENILKSLDRIGEVRKPRVQQAGFVVLKSP
                     DIPSMLVETAFISNREDERKLSLPAHRARLANAIFAGIEQYFQGNAPDGTRLAAARRS
                     AGARVELRK"
     gene            complement(2932..3387)
                     /gene="tsaE"
                     /locus_tag="VEW08_09340"
     CDS             complement(2932..3387)
                     /gene="tsaE"
                     /locus_tag="VEW08_09340"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HYJ40982.1"
                     /translation="MTADVPCAWHTRSGEETEALGASLLGTAERDSPCRVVFLRGELG
                     SGKSTFARGVLRALGVQGPIKSPSYTLLETYELPAITAVHLDLYRLRDAEELEHLGLP
                     DYHRPGFLWLVEWPERGAGRLPEADLQFEFSIGQDGHRIERIETFKTLK"
     gene            complement(3384..>4035)
                     /locus_tag="VEW08_09345"
     CDS             complement(3384..>4035)
                     /locus_tag="VEW08_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HYJ40983.1"
                     /translation="PDNLVAVTATRPELIYLPASASSNLENAIQACDVLAIGPGLGTG
                     DWARRLWAQVRAAGIPVVVDADALNLLAANPGKLPADWIITPHPGEAARLLGIDARAV
                     QADRLGSARELHSRYGAVAVLKGAGTLVCSGGAEPDVAELAICDRGNPGMATAGMGDV
                     LTGVVAGLRAQCGDSGQAARLGVLVHALAGDSAAQGGQRGLIASDVIAELRGWVNP"
BASE COUNT          689 a         1338 c         1330 g          678 t
ORIGIN      
        1 gctgtcgagg tggtggctgg cggaatccgc ggtgacgtcg ccgatcacct cgcgcaccag
       61 cgccggtatg tcgtcggtgg gcagcatggc cggcaccgaa cgcaccacca gcgtttccgg
      121 agccaggcgt tcgacgtcga aacctgccgc gcgccactcc tcgctttgcg tcatcagcgc
      181 atcaagctcg tccactttca tttcgacggt aacgggcacc agcagcgact gcgtggggac
      241 actggcaccc gcctgcactt tcagtttttc atacaacacg cgttcgtgtg cggcgtgcat
      301 gtcgaccagc accatcccct gtgcgttctg cgccaggata tagatgccat gaacctgggc
      361 gagcgccgtg cccagcggct gttcgccgcg aacatcagac gggacgcctt cgacttcatg
      421 cgggtgcggg tccgcgctca tgccccgcaa tgcgtccgca accgcccaga cgccctgggg
      481 ggcggcgcca tccgcggaat gcaatgacat cggttgcgtg tactggctgc cggaggcgta
      541 tgccggcgga accgtgctgg tgccgggacg agtgccggcg agcttgcgtt cgacggcgcg
      601 gaacacgaag tcgtggatct ggcggctatc gcggaaacga acttcgagtt tctgggggtg
      661 cgcgttgacg tcgacgagcg agggatcgat gtcgaggtac aacacgtacg ccggctgccg
      721 tcccgaatac agcacatcgc ggtatccgag acgcacggca ttcatgagca gccggtcgcg
      781 caccgcgcgg ccgttcacga accagaactg catgtcggac gtggcgcgcg ccgccgacgg
      841 cgcgccgatc cagccggaga tgcgcaccgg cccggcggaa tgatcgagct cgatcgcgcg
      901 tgaaacgaat tccgacccga cgatgcgtgc gaggcgctga gcggccccgc cagtggccgt
      961 gagtgccggt gcgtctaaca aagtccgcga accactgcgc aggcggaacg cgacgtcgtc
     1021 gcgcgacagg gccaggcgct cgagcaaccg cacgatgtgg ccctgctcgg tgacctcgct
     1081 gcgcaggaac ttgcgccgcg ccggcaggtt gtagaacagg tcgcgcacct cgatggtggt
     1141 gccggtggga tgcgccgcgg gtgccgggtc gctgacctgg ccggcatcga cggtcagcga
     1201 ggcggcgcgg tcttcatcca cgtgccgcga agtgatttcg aaccgcgata ccgaggcgat
     1261 ggacggcagc gcttccccgc ggaaccccag cgtcatgatg gcgccaagat cgtcggcgtt
     1321 ggcgatcttg ctggtggcgt ggcgcgacat cgcgagcttc aattcggcga cgggaattcc
     1381 gcagccgtcg tcgcggaccc gcgtgagccg cacgccgccc gcctcgattt cgacctcgac
     1441 gctctgcgcg ccggcgtcga ggctgttttc gaccagctcc ttgacgatgg atgccggacg
     1501 ctcgatgacc tcgccggcag cgatctggtc gatgaggtga ttgtcgagta cgcggatcgg
     1561 catggccgcg cagtttaaag ggaaaggggg tcaggcaccg tttcctagaa atcggtgcct
     1621 ggcaccgatt tctaggaaac ggtgcctgac cccattattt gcggagttcg acgcgcgcgc
     1681 cggcgctgcg ccgggcggcg gcgaggcgcg ttccgtcggg cgcattgccc tgaaagtact
     1741 gctcaattcc cgcgaagatg gcgttggcga gcctggcgcg atgcgccggc agcgacaact
     1801 tgcgctcgtc ttcccggttg gaaatgaagg cggtctcgac cagcatcgaa gggatgtccg
     1861 gggacttgag caccacgaag ccggcctgct gaactcgcgg cttgcgcact tcgccgatcc
     1921 gatcgagcga cttcaggata ttttccgcgg ccaccatgct cgccgagatg ctcgcggtct
     1981 gcgacaggtc gagcaacacg ttggcgagca cggcgtcctt gtcatcgagc ttgatgccgc
     2041 ccttcaggtc cgcggcgttt tcacgctcgg ccagccagcg cgccgcctca ctcgaggcgc
     2101 cgcgatcgga caacacgtac accgaagaac ccgacacgtc cgggttggcg atggcgtccg
     2161 catggatgga cacgaacaga tccgccttgg cgcggcgcgc gcgcagggtg cgctccttca
     2221 gcgaaatgaa gtaatcgcca tcgcgcgtga gcaccgcctt catgccgggc tcggcgttga
     2281 tgcgcgcagc cagcgccttg gcgatttcca ggaccacgtt cttttcgcga gtgccgcgtt
     2341 tgccggaagc gcccggatcc acaccgccat gtccggcatc gatggcgatc acgatgtcgc
     2401 ggccggaatc actgggcgca tgcgcgggac gaatggccac cggcgccgcg ggaaccgtgg
     2461 gcgtgacggc gactgtctta gcgacacccg aagcggcggg acccgcaatg tcgaggatca
     2521 ggcgatggcc cgcatcaccc gagggcattg cggcggaagt ctggagggtg actgggcccg
     2581 acacttcgaa gaccaggcga aggccgctat gcggcagctc ccccgagcgc atcgatgtaa
     2641 cgacgccttc ggcgtcgggc agcttcgaga gcagggaaga cttcgacagg tccaccacga
     2701 cgcggtgtgg gctgtcgagc aggaaggctt tgtgtttggc cggggcggac aggtcgagca
     2761 ccacgcgcgt gccggtttcg gtgctggcga tgcggacttc gcggatttct gcggcgacga
     2821 ggttcccgga aactcccgca aagaatgcga gcagcgccac ccaaacccca gttcttgtcc
     2881 ccaaaaaacc cttccccatc atggagttca acgcctgatg tgagacgcaa tttacttaag
     2941 tgtcttaaaa gtttcaatcc tctcgattcg gtgcccatcc tggccaatcg aaaactcgaa
     3001 ttgcaggtcc gcttccggca atcgccccgc tccgcgttcc ggccattcga cgagccacag
     3061 gaaccccggg cggtgatagt ccggcaggcc caggtgctcg agttcttcgg cgtcccggag
     3121 ccggtacaga tcaagatgga ccgcggttat cgccggtagt tcgtaagtct cgagcagcgt
     3181 gtagctcggg ctcttgatgg gtccctgcac gcccagcgcg cgcaacacgc cgcgcgcgaa
     3241 tgtcgatttg cccgatccga gctcgccgcg caggaacacc actctgcagg gtgaatcacg
     3301 ctctgccgtg cctaacaggc tcgcgccaag cgcctcggtt tcttcgccac tgcgcgtgtg
     3361 ccacgcgcac ggcacatctg ccgtcacggg ttcacccagc cacgcaactc ggcgatgacg
     3421 tcgctggcga tgaggccgcg ctgaccaccc tgcgctgccg aatctcccgc cagcgcgtgc
     3481 accagcacac cgaggcgagc ggcctggccg ctgtcaccac attgcgcacg cagtccggca
     3541 acgacgccgg tcagcacgtc gcccatgccg gcggtggcca tgccggggtt accgcggtcg
     3601 cagatcgcca gttcagcaac gtcgggttcg gcgccgcccg aacacacgag cgtacccgcg
     3661 cccttgagca ccgcgaccgc accataccgc gagtgcagtt cgcgtgcgct tcccagccgg
     3721 tccgcctgca cggcgcgggc atcgatgcct aacaacctcg cggcttcgcc gggatgtggc
     3781 gtgatgatcc agtctgccgg cagcttgccg gggtttgccg ccagcaggtt cagcgcatca
     3841 gcatcgacca ctacggggat cccggccgcg cgtacctgtg cccacaggcg gcgcgcccag
     3901 tcgcccgtgc ccagcccggg accgatggcc agcacatcgc aagcctgaat cgcgttctcc
     3961 aggttggagc tggccgaagc aggtagatag atgagctccg gccgtgtggc cgtgaccgcc
     4021 acgaggttat ccggc
//
LOCUS       DASQJS010000232         6858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2644973, whole genome shotgun sequence.
ACCESSION   DASQJS010000232 DASQJS010000000
VERSION     DASQJS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2644973"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VEW08_09350"
     CDS             complement(<1..332)
                     /locus_tag="VEW08_09350"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HYJ40984.1"
                     /translation="MKFVVGIVCVAILNMGMVGAASAAIVDTATLVTPDRAADMSVVR
                     NHLDRADVRAKMEEMGIEATAVDRRLARLNDRELHKLAADMQNAPAGGADAVWIVGAV
                     FIVLIILEL"
     gene            complement(445..1164)
                     /locus_tag="VEW08_09355"
     CDS             complement(445..1164)
                     /locus_tag="VEW08_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40985.1"
                     /translation="MKDFSKLQKTLPLALLLGVVLNGCGGGDSDSPPPAAPAPQPVPS
                     PPPPPPPPPPPPPPPATTVPPLSSTVVSITDNHQIGVDHWSDGNTATGGAGQTVSGLD
                     CLLTMDETYHVHTHVSIFLNGEALSVPADLGIVPAAPQGRCFYSIHTHDKSGKIHIEA
                     AAPGTFTLGQLFAIWGQPLMNTNIAGMTGLPVSVYVTDNGTVTEAVDNWQSIELTSHR
                     EITIAVGTPITEIPNFTWSGN"
     gene            1643..2551
                     /gene="rarD"
                     /locus_tag="VEW08_09360"
     CDS             1643..2551
                     /gene="rarD"
                     /locus_tag="VEW08_09360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HYJ40986.1"
                     /translation="MTRITGGGGFAAAIAAFAIWGLFPLYLIGLMNVSAMQITAHRIV
                     WSCVFVLAWLAVRQELGTLWASVARKGVLLRLMASAFFIAVNWVGFAWAVNNDHVLEV
                     SLAYFMGPLLNILLGIFVLSERLDRTQWIAVGFAAAGVVYLTFIAGHAPWLALMVGSS
                     FALYGVIRKTVSIDALPGLAIETILLVPFAAGYLIWCEAHGIGALGHQSGRVDALLLA
                     GGVVTSVPLFLFSYGARRVPYSTIGVIQFIAPTLQLVCGLVVFGETFESARATGFVLI
                     WIGLLIYIGNALWRASSLRPATTSGN"
     gene            complement(2540..3361)
                     /locus_tag="VEW08_09365"
     CDS             complement(2540..3361)
                     /locus_tag="VEW08_09365"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HYJ40987.1"
                     /translation="MNPYLTAARFMRVFDFNSAIVRTPGRSVVNGLRANDGPAPSFET
                     VVAEHQAYIAALRAAGVRVTVLDALEQFPDSIFVEDPALVFSQAALLLRPGAPSRMAE
                     ARELSSTLAARFPEVLQLSDGFADGGDILVTPSRILIGLSARTNEAGAADLQKLLKSI
                     GLASRVVQVPRGTLHLKTDCSLIDEETVLSTKGLAKSGVLDGLRVLVVPEEERSATNA
                     LRINDVVFIRAGCPRTQDMLVTHGLKVVSLPVSEIGKIDAGLSCMSLRWFDRLVA"
     gene            3388..5448
                     /locus_tag="VEW08_09370"
     CDS             3388..5448
                     /locus_tag="VEW08_09370"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HYJ40988.1"
                     /translation="MNPDMHKTSFRALRGLAALSLCFVFGLAYAQADAYPDADPPDRA
                     ARLSYLEGDVSLQPAGEEEWAAAIVNRPLTTGDKLWTDQGARAEIQVGRAAVRLGSGT
                     GFSFLNVDDNTIQMRVTAGVINVSVRDLAGNEHIEIDTPNLSLSLLRPGNYRVEVNDN
                     GDSTIVKVSEGMAEANGGSQNAVVHAQQVVTFNGVDQVIAQIGTLGAPDEFDSWSLER
                     ERRDERAETSQTARYVAPDVTGYEDLEDNGTWNSEPEYGYVWTPRVAIGWAPYSYGRW
                     AWVSPWGWTWIDDARWGYAPFHYGRWAHVRNRWCWVPGPRHVRAVYAPALVGWGGSHG
                     GHVSWFPLGPREVYVPGRHYSRRYFERVNVANTTVNRSRFAHAIADRVEDTVYRNRVV
                     PGGVTAVPRATFASAGHTGANRLRVSEQEQASSLASAIAPQITPVRASRSGERRANVR
                     IPPQNIANRQVVVRRDPPPAAARYARRPPVSAGLAQAPDRSARPPVRGQVNDAADRGN
                     GREAVVRNYHDRPPRTEPTTQSAPSVQTSVFDRRAIANAVQEDRERQGREGQRQREAL
                     QQREAMQQRDASQQREAQPQRPWQRQEDQARQQREQTQASRELQNRVREHAQRVQEVQ
                     RPAAPRPPEQRERPAQRERNAERPQAERPQVERPRSEARPPPAETRSQPRNREEGNGR
                     SKRN"
     gene            5580..6161
                     /locus_tag="VEW08_09375"
     CDS             5580..6161
                     /locus_tag="VEW08_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40989.1"
                     /translation="MNNKGLFVSLVLSGGLAGLAVFPGPAVGDQGQSASQQNQLKQHS
                     GELVRVVRESTRRFRDVNQAEAEGYHLLFGCVSGPDDGAMGLHFVNMELVIDPALDPR
                     KPEIVIYEPLPNGGRRLIGADFLVFAEAWHANNTGTPELGGQLMHLIESPNRYGLPAF
                     YTLHVWAWKPNPTGAFVNWHSDVSCEQFDGGQQ"
     gene            6164..>6858
                     /locus_tag="VEW08_09380"
     CDS             6164..>6858
                     /locus_tag="VEW08_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ40990.1"
                     /translation="MSTTIYRGSVFLFSIVCYGVFLLVFLYLLAFLGNLQATPLADAF
                     PILRTLVPRSVDLGHDMGSAGMAVAVDVGLILLFGLQHSVMARPGFKRVWTRIVPKEM
                     ERSLYVLLSSAVLAFFMWQWRPIPMPVLWHADTAWSVALGWSVMGLGVAVLLWATFLI
                     NHFELFGLQQGWITLRGGELRGPAFVTPYLYKIVRHPLYVGWLLIFWGAPTMTAGHLL
                     FSVGMSGYILLAIR"
BASE COUNT         1257 a         2137 c         2182 g         1282 t
ORIGIN      
        1 agctcgagga tgatcagcac gatgaacacc gcgccgacga tccagacggc atcggcacca
       61 ccggcgggcg cgttctgcat gtcagcagca agcttgtgca gttcgcgatc gttgagcctg
      121 gcgaggcggc ggtcgaccgc cgtcgcttcg attcccattt cctccatctt ggcgcgaacg
      181 tccgcgcgat cgagatggtt gcgtacgacg ctcatatccg ctgcgcgatc cggcgtgacc
      241 agggtggcgg tatccacgat ggccgcagaa gcggcaccca ccatgcccat gttgaggatt
      301 gcgacgcaaa caatgccgac tacgaatttc acagaaacct ccgaatgatg agcaagcgcg
      361 taatcttcaa caaaagcggc gaatagaaat gtaggacgtc aggagagcat gtccaaaatg
      421 acttgatgct cccctcgcgc ccggtcagtt accgctccac gtgaagttgg ggatctcggt
      481 gattggcgtc cctaccgcaa tcgtgatctc gcgatgagac gtcagctcga tgctctgcca
      541 attgtcgacg gcctccgtca ccgttccgtt atcggtgacg tacaccgaca cgggcagtcc
      601 ggtcatcccg gcgatattcg tgttcatcag tggctggccc cagatggcaa acagctggcc
      661 gagcgtgaac gttcctggcg cggccgcttc gatgtggatc tttccagact tgtcatgagt
      721 atggatcgag tagaagcagc ggccttgagg cgccgcgggc acgatcccaa ggtcggccgg
      781 aaccgagagt gcttcgccgt tcaggaaaat ggagacatgg gtgtggacgt gatacgtctc
      841 gtccatggtg agcaggcagt cgaggcccga aacggtctgg cctgctccgc ccgtggccgt
      901 gttgccatcg ctccaatgat cgacgccgat ctggtgattg tcggtgattg aaactaccgt
      961 cgaggacaac ggcggaactg tcgtagccgg cggtggtggc ggtggtggtg gtggcggcgg
     1021 cggcggtggc ggggacggca cgggctgagg cgcaggcgcg gccggcggtg gtgaatcgga
     1081 atcgccgcct ccacagccat tgagcacgac gcctaacagc agcgccaacg gcaaagtttt
     1141 ctgcagcttc gaaaagtctt tcatggaaat tccgcccttg tcatcgtttg ttgaccggga
     1201 ttgaatttcc ttcaaatcgg gctgcaggct ggacagacgc gacccgcagg atttgtaggg
     1261 cacgccgcag tgacgtcagg cagccagtcg gacaaaagat gcattcgcgc catggcgcgt
     1321 cagacaagga gaaatacatg ggcacgaggt tattgttagg agtggctctc gcagccctaa
     1381 tgctgtctct gtccggcgtc acccaggctt cggagtccat gaagacgaag ccgcgcacaa
     1441 agcgcacatc gcatctgcgc atttcaggaa gtacgtagag attacgaaag acatgatccg
     1501 ctcccgcaag ctgctggata cggtgccggt tcagctaagc gcgaagcagg ctcatgggcc
     1561 ctccaatgct ctcggggtca cgacgccggg gtctctcgtt cggtagacgc agcgcttgcg
     1621 gttcttcggc aaaatggctg gcatgactcg aatcacaggc ggtggtggat ttgcggcagc
     1681 gatcgccgcg ttcgccatct ggggactgtt tcccctctat ctaattggcc tcatgaacgt
     1741 gtcggccatg cagatcaccg cgcatcgcat cgtctggtcc tgtgtgttcg tgctcgcctg
     1801 gctcgcggtg aggcaggagc ttgggaccct gtgggcatcc gtggcgcgca agggtgtcct
     1861 tctgcggctg atggccagcg catttttcat cgcggtcaat tgggtcggct tcgcgtgggc
     1921 ggtcaacaat gaccacgtgc tcgaagtgag ccttgcgtac ttcatgggtc cgctgctcaa
     1981 catcctgctc ggcatcttcg tgctctcgga acggctggat cgcacgcagt ggatagccgt
     2041 gggattcgcg gccgcgggcg ttgtatacct cacattcatc gcgggccacg cgccatggct
     2101 cgcgctcatg gtcggtagtt ccttcgcgct ctatggcgtg attcgcaaga cggtgagcat
     2161 cgacgcgctg cccggactcg cgattgagac gattttgctc gtgccgttcg ccgctggtta
     2221 cctgatctgg tgcgaggcgc acggcatcgg ggcgctagga caccagagcg gacgagttga
     2281 tgcgttgctc ctcgccggag gagtcgtcac ttcggttccg ctgttccttt tctcctacgg
     2341 cgcacgacgc gtgccgtatt cgaccatcgg ggtgatccag ttcatcgccc cgacgctgca
     2401 gctggtgtgt ggcctggtcg tattcggaga gacgttcgaa tcggcgcgcg ccacgggatt
     2461 cgtgctcatc tggatagggc tgctgatcta catcggcaat gcgctgtggc gcgcaagttc
     2521 gctcagacct gcgacgactt caggcaacta gccgatcgaa ccagcgcagc gacatgcacg
     2581 agagcccggc atcgatcttg ccgatctcgg acaccggcag cgacaccacc ttcaatccat
     2641 gcgtcacgag catgtcctgg gtgcgcgggc accccgccct gatgaacacg acatcgttta
     2701 tccggagcgc attggtggcg cttcgctcct cctccggtac gacgagaacc cgcagaccat
     2761 ccaggactcc ggatttcgcc agccctttgg tcgacagcac tgtctcttcg tcgatcagcg
     2821 aacaatccgt cttgaggtgc aacgtgccgc gcggcacctg aaccacccgg ctggcgaggc
     2881 caatggattt gagcaatttc tgcaaatcag cggcgccggc ctcattcgtt cgggcagaga
     2941 ggccaatgag aatgcggctg ggcgtgacga gaatgtcgcc cccgtccgcg aagccatcgc
     3001 tcaattgcag aacctcgggg aatcgcgccg cgagagtcga ggacagctcc cgcgcttccg
     3061 ccattcggct cggcgctccg ggccgcaaca gcagtgccgc ctggctgaac accagcgcgg
     3121 gatcctcgac aaatatcgag tccggaaact gctccagcgc gtcgagaacg gttacccgta
     3181 cgcccgccgc ccgcaacgcg gcgatgtatg cctggtgttc ggccaccacc gtttcgaagc
     3241 tgggcgccgg cccatcgttg gcgcgcaggc cgttgaccac gctcctcccg ggcgtacgga
     3301 caatggcgga gttgaagtcg aaaactcgca tgaatctggc ggccgttaag taagggttca
     3361 ttctatgcgc cctatattgg gtccagaatg aacccggata tgcacaagac gtcattcaga
     3421 gccctgcgtg gcctggcggc actctcgctc tgcttcgtgt tcgggctggc ttacgcgcag
     3481 gcagacgcgt accccgatgc cgacccccct gatcgggccg ctcgtctgag ctacctcgag
     3541 ggcgatgtgt ctctgcagcc cgcaggcgag gaagaatggg ccgccgcaat cgtcaatcgg
     3601 ccgctgacca ccggcgacaa gctgtggact gatcaaggcg cgcgcgccga gatccaggtc
     3661 gggcgtgccg ccgtccgcct cggcagcggc acgggattct cattcctgaa tgtcgacgac
     3721 aacaccatcc agatgcgcgt caccgcgggc gtcatcaacg tgtccgtgcg ggatctcgcg
     3781 ggcaatgaac acatcgagat cgacacgccc aatctttcgt tgtcgctctt gcgccccggc
     3841 aactaccgcg tcgaagtgaa cgacaacggc gactcgacca tcgtcaaggt cagcgaaggc
     3901 atggccgaag cgaacggtgg atcgcagaac gcagtcgtac atgcacagca ggtggtgacg
     3961 ttcaatggcg tagatcaggt gatcgcgcaa atcggcacgc tcggtgcgcc ggacgaattc
     4021 gattcctgga gtctcgagcg cgagcggcgc gacgagcgtg cggagacatc acaaacggcc
     4081 cggtacgtgg cgcccgacgt cactggctac gaagatctcg aagacaacgg cacgtggaac
     4141 tccgaacccg aatacggata cgtgtggaca ccgcgcgtcg ccattggctg ggcgccatac
     4201 agctacggcc gctgggcctg ggtttcaccc tggggctgga catggatcga tgacgcgcgc
     4261 tggggctacg caccgtttca ctacggccga tgggcgcacg tccgcaatcg ctggtgttgg
     4321 gtgcccgggc cgcggcacgt gcgcgcggtg tacgcgccgg cgctcgtggg ttggggcggc
     4381 tcgcatggtg gccacgtctc gtggtttccg ctcggcccgc gcgaagtcta cgtgccgggg
     4441 cggcactaca gccgccgcta cttcgagcgc gtaaacgtcg cgaacacgac agtgaatcgg
     4501 tcgcgcttcg ctcacgccat cgccgacagg gtcgaggaca ctgtctatcg aaaccgcgtg
     4561 gtgccgggcg gcgtgacggc ggtgccgcgc gccacgtttg cctccgcggg ccataccggt
     4621 gcgaatcgcc tgcgcgtgag cgaacaggaa caggcgagca gtcttgcaag cgcaatagca
     4681 ccgcagatca cgccggtgcg cgcgagccgt tcgggcgagc ggcgcgccaa cgtgcgcata
     4741 ccaccgcaga acatcgcgaa ccgccaggtc gtcgtgagac gggatccgcc ccccgcggct
     4801 gcgcgttacg cgcggcgtcc gccagtcagt gcaggcctgg cacaggcgcc ggatcgctcg
     4861 gctcgaccgc cggttcgcgg ccaggtcaac gacgctgctg accgcggcaa tggccgtgaa
     4921 gccgtcgtcc gcaattatca tgatcgcccg ccccgtaccg agccgaccac ccaatccgcg
     4981 ccgagcgtgc agacgtccgt tttcgatcgg cgcgcaattg ccaatgcggt gcaagaggat
     5041 cgcgagcgac agggacgcga gggacagcgg cagcgtgaag ccttgcagca acgtgaagcg
     5101 atgcaacagc gtgatgcctc gcaacagcgt gaggctcagc ctcagcggcc gtggcagcgg
     5161 caggaagatc aggcgcgcca acagcgcgaa cagacgcaag cgtcgcgcga actgcagaat
     5221 cgagtgcgcg agcacgctca gcgtgtgcag gaagtgcagc ggccggccgc gccgcgaccg
     5281 ccggaacagc gtgagcggcc agcacaacgt gagcgcaacg ctgaacggcc gcaggccgaa
     5341 cgcccacagg tcgagcggcc tcgctccgaa gcacgccccc cgccggcaga gacgagatca
     5401 cagccgcgca atcgagaaga aggaaacggg cgttcgaagc ggaattgatt gaccttgctt
     5461 ctttcgcccc ggggtttgaa aacccgtagc taatccgtat cccgtcctga ggactcctga
     5521 ggcgccctgc gactctagtc acctctcctc attcattcag agacaggagg gatacggtca
     5581 tgaacaacaa agggttattc gtctcgttgg tactttccgg cggcctggca ggcctggccg
     5641 tcttccccgg cccggcggtc ggggaccagg gccagagcgc cagccagcag aatcaactga
     5701 agcaacattc gggcgagctg gtgcgcgtgg tccgcgaatc cacgcgacgt tttcgcgacg
     5761 tcaaccaggc ggaggccgag ggataccacc tgctgttcgg atgtgtcagc ggccccgacg
     5821 acggcgcgat gggattgcac ttcgtcaaca tggagctggt gatcgacccc gcgctggacc
     5881 ctcggaagcc ggaaatcgtc atctacgaac cgctgcccaa cggtggccgc cgcctgatcg
     5941 gggcggactt tctcgtattc gcggaggcct ggcatgccaa caacaccggc acaccggagc
     6001 ttggcggcca gctcatgcac ctgatcgaga gcccgaatcg ttatggtctg ccggccttct
     6061 acacgctgca tgtctgggcg tggaagccga acccgaccgg tgcattcgtg aactggcatt
     6121 ccgacgtgtc ctgcgaacag ttcgacggcg ggcagcaata atcatgtcga ccactatcta
     6181 ccgcggcagc gtgttccttt tcagcatcgt ctgttacggc gtgttcctgc tggtattcct
     6241 gtacttgctc gcattccttg gaaacctgca ggcaacgccg ctggcggacg cctttcccat
     6301 actgcgcaca ctcgtgccca gatccgtcga tctcgggcac gacatgggtt cggcgggaat
     6361 ggcggtcgcg gtggatgtcg gtctgatcct gcttttcggc ttgcagcatt ccgtgatggc
     6421 gagacccggg ttcaagcgcg tctggacccg gatcgtgccg aaagaaatgg agcgcagcct
     6481 gtacgtgctg ctctcgagtg ccgtcctcgc gttcttcatg tggcagtggc gccccattcc
     6541 catgcctgtg ctctggcacg cggacacggc ctggagcgtc gctctcggct ggtcggtcat
     6601 gggcctcggc gtcgccgtgc tgctgtgggc cacgttcctg atcaatcact tcgaactatt
     6661 tggactgcag cagggctgga tcacgctgcg cggtggagag ctgcgcggcc ccgcattcgt
     6721 cacgccgtat ctctacaaga tcgtgcgtca ccccctgtac gtgggctggc tgctgatctt
     6781 ctggggcgct ccgacgatga ctgccggtca cctgctgttt tcagtcggca tgagcggcta
     6841 catccttctc gccatccg
//
LOCUS       DASQJS010000233         7169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4530111, whole genome shotgun sequence.
ACCESSION   DASQJS010000233 DASQJS010000000
VERSION     DASQJS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4530111"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..2049)
                     /locus_tag="VEW08_09385"
     CDS             complement(97..2049)
                     /locus_tag="VEW08_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYJ40991.1"
                     /translation="MNRKILLTLVMAGAWNLVHGLAQGAVEERGNLVLDNVPPVDTPL
                     TAKLDDYMNSRGASFVDWLPDGGVLIATRFGDVEQLHRVAMPLGAREQLTFYREPVTN
                     ARSPQSAVASGFAFLKDQGGNENAQVYWYETATRAVRVLTDGKGLHGGLTWSHDGNRV
                     AFHGTGRDGVSYDLYIAEPANKFAQPRLVFNGFQKNWSVQDWSPDDTRLLINNFVSAN
                     ESHLYVMDIATAALTPVSEGKDIDDTASVSQAKFTPDSRGVYLVTNRDSEFRQLRRVD
                     LPTGAVETLTGHIPWDIDTFARSDDGKYLAWVANVDGQSRLTVLNIATRSESVPPLPD
                     GEIGRIAFDRTGKKLGLSLESAQSPRDVFVLELERNALVRYTKSEAGPIDPLQFVPAE
                     LVRFPTFDREGGKNRQIPAFIYRPRTPGPHPVFIDIHGGPEAQYTPSFTPFTQFLVRE
                     LGFIVIAPNVRGSTGYGKTYMNLDNGEDREDSVKDIGALLVWIGAQKDMDAKKVFVAG
                     GSYGGYMSLAAMVNFGDRLRGGIDVVGISNFVTFLESTSAYRRNLRRPEYGDERLPKM
                     RAYLQRISPLTNAARISRPLLVVQGLNDPRVPATESQQMVAKIRARGGDVWYLAAKDE
                     GHGFRKKPNRDFYLKTMVTFLEKYSK"
     gene            2083..3021
                     /locus_tag="VEW08_09390"
     CDS             2083..3021
                     /locus_tag="VEW08_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ40992.1"
                     /translation="MPRLTTFADGITAVDTEYVRPQMDASHIVVAGDRAAVVDTGPNT
                     AVPLILAALEQLHIAPDAVELLFLTHVHLDHAGGAGALMRALPRATCVVHPRGAPHMI
                     DPGKLIAGTRAVYGDELYSKLYGEILPVDRERLAIAQDGQRFEIPGRQFECVHTPGHA
                     LHHQAIIDHATTSIFTGDTFGLSYREFDTAKGAWIMPTTTPTQFDPGQLKGSIMRLMQ
                     FRPRHLYLTHYSEVSECARLANDMVDAIDDFVKIARAAGPDDLPRMRGDLRRLAHEGL
                     REHGCTMSDAQIDAILSKDFELNADGLVAWLGRVAN"
     gene            3023..3490
                     /locus_tag="VEW08_09395"
     CDS             3023..3490
                     /locus_tag="VEW08_09395"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HYJ40993.1"
                     /translation="MLYLALKSLHVISVVLFLGNIITGVFWKVHADLSGDLRVRQQAI
                     LGLIRSDRFFTVPGVLLIIATGVALALIARLPLLRTFWIAWSLGLFGISGLAFAFKVG
                     PLQKKLLANTSAGLAGTWNEAEYRALSKSWGIWGTIATAAPLIAVFLMVMKPA"
     gene            3622..4884
                     /locus_tag="VEW08_09400"
     CDS             3622..4884
                     /locus_tag="VEW08_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYJ40994.1"
                     /translation="MPRLHVPQPPTRPGDKPDFSYVELSPAGAINKPDVNARARDMEE
                     VSVGLVRVLDDKHEAVGQWNPRLDAEDLQVGLRHMLLTRIFDDRMQRTQRQGKISFYM
                     RCYGEEAVGVAQAMALQPGDMLFPSYRQQGIYMVRGKPLVELMCQLLSNTRDMCKGRQ
                     LPVMYHWNAGRIFSISGNLTTQFPQAVGWAMAAAIRNEDHIACTWIGEGSSAEADFHH
                     GIIFAAVHQAPVIMNIVNNQWAISTFQGTAGGEQRSFAARGPGYGIAGIRVDGNDFLA
                     VYAVTQWAAQRARTGGGPTLIELVTYRASAHSTSDDPSRYRPKDDYEHWPLGDPVDRL
                     KNHLIARGEWSEKQHKDLHAELDAQVVTAWKEAVSFGALNEGPRLDRHLMFEDVYKEF
                     PENLRRQSEQLAEEIKLNAELGLVAKVD"
     gene            4887..5900
                     /locus_tag="VEW08_09405"
     CDS             4887..5900
                     /locus_tag="VEW08_09405"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYJ40995.1"
                     /translation="MAQMNMIQALNSAMDVMMARDPSVVVMGEDVGYFGGVFRVTDGL
                     QRKYGDQRVLDSPISEGGIVATAIGMAVNGLKPVAEIQFADYIYPAVDQIASELARIR
                     YRSAGDFSSPVVIRAPCGGGIRGGQTHSQSPEAIFTHICGVKVVMPSNPYDAKGLLIA
                     AIEDEDPIIFFEPKRIYNGPFDGDPNKPAVPWSTHAKGNVPEGHYKVPIGKCDIVRPG
                     SEVTIVTYGTMVYVCEAAAKKLNIDAEIIDVRTMSPLDIDPICSSVKKTGRCVVAHEA
                     TRFSGFGAELSASIQEECFWYLEAPIQRVAGWDTPYPHAFEWEYFPGQARVALALRAA
                     MGE"
     gene            5904..>7169
                     /locus_tag="VEW08_09410"
     CDS             5904..>7169
                     /locus_tag="VEW08_09410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HYJ40996.1"
                     /translation="MSRYVFKMPDLGEGTVSAEVVAWHVKPGDLVQEDQVMCEVMTEK
                     AAVEMPAPVTGRILSIQGEPGDMVAVGSELVVFDTDATSAAAGDAPAARTPPPVPAEP
                     LTPKAGEKQQVAAVAAINGTNGDGKTARVMVSPASRRRAAEAGLDLSTVAGTGPAGRI
                     EPGDIDSALAAGAARGAPPSINARANAPHAPRNGTEEVKIIGLRRVIAERLTESARSI
                     PQYSYVEECDLTRLEGLRKHLNDRRPPSAPPLTFLPFIVAALARVLDKFPQCNALYDK
                     ERGVLVKHKAVHVGVATQTPQGLKVPVVRNAESRALHDLAAEIRRVAEAARTNKSPRE
                     ELTGSTITITSLGKLGGIASTPMINMPEVSIIGINKAIERAVVVDGQVTIRLMMNLSS
                     SFDHRFVDGFDAASMIQALKDSLEQPATIF"
BASE COUNT         1311 a         2358 c         2241 g         1259 t
ORIGIN      
        1 ctcgagaccg ttcccaaaaa ggtctttttc ttcaactagt caggcgctgc gcgccacgtc
       61 ggaaaagccg cactaacggg cggcgtccga aaaacctcac ttcgaatatt tctcgaggaa
      121 cgtcaccatc gtcttcaggt agaagtcgcg attcggcttc ttcctgaacc cgtggccttc
      181 gtccttcgcc gcgagatacc agacgtctcc gccgcgcgcg cggatcttcg cgaccatctg
      241 ctgcgactcg gtcgcgggca cgcgcgggtc gttgagcccc tgcaccacga gcagcggtct
      301 ggaaatgcgc gccgcgttgg tgagcggcga aatcctctgt agataggcac gcatcttcgg
      361 cagccgctcg tcgccgtatt ccggccggcg caggttgcgg cgataagccg aggtgctctc
      421 gaggaaggtg acgaagttcg agatgccgac cacgtcgatg ccgccgcgca gccggtcgcc
      481 gaagttcacc atcgcggcca gcgacatgta gccgccatac gagccaccgg cgacgaacac
      541 cttcttcgca tccatgtcct tctgcgcccc gatccacacg agcagcgcgc cgatgtcctt
      601 gaccgaatcc tcgcggtctt cgccattgtc gagattcatg tacgtcttgc cgtagccagt
      661 ggaaccgcgc acgttcggcg cgatcacgat gaacccgagc tcgcgaacca ggaactgcgt
      721 aaagggcgtg aatgacggcg tgtactgggc ttcggggccc ccgtggatgt caatgaagac
      781 cgggtgcggg ccgggcgtgc gcgggcggta gatgaacgcg gggatctggc ggttcttgcc
      841 accctcgcga tcgaaagtgg ggaaacgcac gagctccgcc ggcacgaatt gcagtggatc
      901 gatgggaccg gcctcactct tcgtgtagcg caccagcgcg ttacgttcga gctccagcac
      961 gaacacatcg cgcggcgact gggcgctttc gagcgacagg cccagcttct tgccggttcg
     1021 atcgaaagcg atgcggccga tctcgccgtc gggcagcggc ggcacactct cgctgcgagt
     1081 ggcgatgttc aacacggtga ggcggctctg gccgtcgacg ttcgcaaccc acgcgagata
     1141 cttcccgtcg tcgctgcgcg caaaggtgtc gatatcccac ggaatgtggc ccgtcagtgt
     1201 ttccaccgcg cccgtcggca ggtccacgcg ccgcagctgc ctgaactcgc tgtcccggtt
     1261 ggtgaccagg tagacaccac ggctgtccgg cgtgaacttc gcctgcgaga cactcgccgt
     1321 gtcgtcaatg tccttgcctt cgctcaccgg cgtgagcgcc gcggtggcga tgtccatcac
     1381 gtacagatgt gattcgttgg ccgacacgaa gttgttgatg agcagccggg tgtcatcggg
     1441 tgaccagtcc tgcaccgacc agttcttctg aaagccattg aacacgagac ggggctgggc
     1501 aaacttgtta gccggttcgg cgatgtagag gtcgtaactg acgccgtcac ggcctgtgcc
     1561 gtggaaggcg acgcggttgc cgtcgtggct ccaggtcagc ccgccgtgca ggcccttgcc
     1621 gtcggtcagc acgcgcaccg cgcgcgtggc ggtctcgtac cagtaaacct gcgcattttc
     1681 attgccgccc tggtccttga ggaacgcgaa gccggacgcc accgccgatt gcggcgaacg
     1741 cgcgttggtc accggttcgc gatagaacgt gagctgttcg cgcgctccca gcggcatggc
     1801 cacgcgatgc aactgctcca cgtcgccgaa gcgggtggcg atgagcacgc cgccatcggg
     1861 cagccagtcg acgaaactcg cgccgcgcga gttcatgtag tcgtctaact tggccgtgag
     1921 tggcgtgtcc acggggggaa cgttgtccag caccagattg ccgcgttctt cgacggcgcc
     1981 ctgggccaat ccatggacaa ggttccacgc gccggccatg acaagagtca ggagtatttt
     2041 tcgattcatg ctgctatgtt gccgcaagcc atgaatgcag tcatgccgcg actgacgaca
     2101 ttcgccgacg ggatcaccgc cgtcgacacg gaatacgtgc gcccgcagat ggatgcctcg
     2161 cacatcgtcg tcgccggcga ccgggcggcg gtagtcgata ccggccccaa taccgcggtt
     2221 ccgctgatac tcgcggcgct cgaacaactg catatcgctc ccgatgcggt ggagctgctg
     2281 ttcctcaccc acgtgcacct cgatcatgcg ggcggcgccg gcgctctcat gcgcgcgctg
     2341 ccgcgcgcta cctgcgtggt gcatccgcgt ggtgcgcctc acatgatcga tcccgggaaa
     2401 ctcatcgcgg gaacccgggc ggtttacggc gacgagctct attcaaagct ttacggcgaa
     2461 attctgccgg tcgatcgcga acgtctggcc atcgcgcagg acggccagcg cttcgagatt
     2521 cccggccgcc agttcgaatg cgtgcacacg ccggggcacg ccctgcatca ccaggccatc
     2581 atcgatcacg ccaccaccag cattttcacc ggtgacacgt tcggcctttc gtatcgcgag
     2641 ttcgatacgg cgaagggggc gtggatcatg cccaccacca cgcccacgca gttcgatccc
     2701 gggcagctca agggatccat catgcggctg atgcagttcc ggccgcgcca cctctatctc
     2761 acgcactaca gcgaagtgag cgaatgcgcg cggctggcca acgacatggt cgacgccatc
     2821 gacgatttcg tgaaaatcgc ccgcgccgcg gggccggacg accttccgcg catgcgtgga
     2881 gacctgcgcc ggctggctca cgaaggcttg cgcgaacatg gctgcaccat gagcgacgcg
     2941 cagatcgatg ccatcctcag caaggatttc gagctcaacg cggacgggct ggtcgcctgg
     3001 ctcgggcgag tggctaacta gcatgttgta ccttgcgttg aaatccctgc acgtcatcag
     3061 cgtcgtgctg tttctcggca atatcatcac gggtgtgttc tggaaagtgc acgccgacct
     3121 gagtggcgat ctgcgcgtgc gtcagcaggc gatcctcggc ctcatccgca gcgacaggtt
     3181 cttcacggtt cccggcgtat tgctcatcat cgccaccggc gtcgcgctgg cgctgattgc
     3241 ccgcttgcca cttctgcgca cgttctggat cgcgtggtcg ctggggttgt tcggcatctc
     3301 cgggctggct tttgctttca aggtggggcc gctgcagaag aaactgctgg cgaacacgag
     3361 tgccggactc gcaggaacct ggaacgaggc tgaataccgc gcgctgtcga agtcctgggg
     3421 tatctgggga acgatcgcga cggctgcgcc cctgatcgcg gtgttcctga tggtgatgaa
     3481 gccggcgtga tgcaagggcc ccgcccgcct ggagctcgcc gttccggcgc agacctgatc
     3541 agtttcacct gaaaccaatc atcgaggggt tcccgtctat cctcagcgcg cctttcttct
     3601 cacccgcgct atcatcgcgc aatgccaaga cttcacgttc cgcaaccgcc cactcgcccg
     3661 ggcgacaagc cggacttcag ctacgtcgag ctgtcgcccg ccggcgccat caacaaaccc
     3721 gacgtcaatg cgcgcgcccg cgacatggaa gaagtgtcgg tgggcctggt tcgcgtgctc
     3781 gatgacaagc acgaagccgt gggccagtgg aatccgcgcc tcgatgccga agacctgcag
     3841 gtgggtctgc gccacatgct gctcacgcgc atcttcgacg accgcatgca gcgcactcag
     3901 cgccagggaa agatctcctt ctatatgcgc tgctacggcg aggaagccgt gggagtggcc
     3961 caggccatgg cactgcagcc cggcgacatg ctgtttccgt cctaccggca gcagggcatc
     4021 tacatggtgc gcggcaagcc gctggtcgag ctcatgtgcc agctgctgtc caacacgcgc
     4081 gacatgtgca agggccgcca gctgccggtc atgtatcact ggaacgcggg ccgcatcttc
     4141 tcgatctccg gcaacctaac tacccagttc ccgcaggccg tgggctgggc catggcagcg
     4201 gccatccgca acgaagacca catcgcctgc acctggatcg gtgaaggctc gagcgccgag
     4261 gccgatttcc atcacggcat catcttcgcg gccgtgcacc aggcgccggt catcatgaac
     4321 atcgtcaaca accagtgggc catctccacg ttccagggaa cggcgggtgg cgagcagcgt
     4381 tcgttcgcgg cgcgcggtcc gggttatggc atcgcgggaa tccgcgtcga cggcaacgac
     4441 ttcctcgcgg tgtacgcggt cacccaatgg gcggcccagc gggcgcgcac cggcggcgga
     4501 cccacactca tcgagctcgt cacctatcgc gcatccgcgc attcgaccag cgacgatcca
     4561 tcgcgttatc gccccaagga cgactacgag cactggccgc tgggcgaccc ggtggataga
     4621 ttgaagaatc acctgatcgc gcgcggcgag tggagcgaga agcagcacaa agacctgcac
     4681 gccgagctcg atgcccaggt ggtcacggcg tggaaagagg ccgtgtcgtt cggcgcgctg
     4741 aacgagggcc cgcggcttga ccgccatctc atgttcgagg acgtctacaa ggagttcccg
     4801 gaaaaccttc gccgccagag cgagcagctc gccgaggaaa tcaaactcaa cgccgagctc
     4861 gggctcgtcg cgaaggtgga ttgaccatgg cgcaaatgaa catgatccag gccctgaact
     4921 ccgccatgga tgtgatgatg gcgcgagatc cttccgtcgt cgtcatggga gaagacgtgg
     4981 gctattttgg tggcgtgttc cgcgtcaccg acggcctgca gagaaaatac ggcgaccagc
     5041 gcgtgctcga ttcgccgatt tccgaaggcg gcatcgtcgc cacggccatc ggcatggcgg
     5101 taaatggcct caagcccgtc gcggaaatcc agttcgccga ctacatctat cccgcggtgg
     5161 accagatcgc gagcgaactg gcgcgcatcc gttaccgcag cgccggcgat ttctcctcgc
     5221 ccgtcgtcat tcgcgcaccg tgcggcggcg gcatccgtgg cgggcagacg cattcgcaga
     5281 gtcccgaggc catcttcacg cacatctgcg gcgtgaaagt ggtgatgccg agcaatccct
     5341 acgacgccaa gggtctgctc atcgcggcca tcgaagacga agacccgatc atctttttcg
     5401 aacccaagcg catctacaac gggcctttcg acggcgaccc taacaagccc gcggtgccgt
     5461 ggagcacgca cgccaagggc aacgtgcccg aaggtcacta caaagtgccc atcggcaagt
     5521 gcgacatcgt gcggccgggc agcgaggtca ccatcgtcac ctacggcacg atggtgtacg
     5581 tgtgcgaagc ggccgcgaag aagctcaaca tcgacgccga gatcatcgac gtgcgcacca
     5641 tgtcgccgct cgacatcgat cccatctgct cgtcggtcaa gaagaccggg cgttgcgtgg
     5701 tggcgcatga ggccacgcgt ttctccggtt tcggcgcgga actttcggcc agcatccagg
     5761 aggagtgctt ctggtacctc gaagctccga tccagcgagt ggccggctgg gacacgccat
     5821 acccgcacgc gttcgaatgg gagtattttc ccggccaggc acgggtggcg ctcgccttgc
     5881 gcgcagccat gggggaataa gatatgagcc gctacgtttt caaaatgccg gacctcggcg
     5941 aaggcacggt ttccgccgaa gtggtcgcat ggcacgtgaa gcccggcgat ctggtgcagg
     6001 aagaccaggt catgtgcgaa gtcatgaccg aaaaggccgc ggtggaaatg cccgcgccag
     6061 tcacgggccg cattctttcc atccagggtg aacccggtga catggtggcc gtgggctcgg
     6121 agctcgtggt cttcgacacc gacgccacgt cggccgccgc gggtgacgcg cccgccgcca
     6181 ggactccacc gccggtgccg gcagaaccac tgacgccgaa ggccggagag aagcaacagg
     6241 ttgccgccgt tgcagcgatc aacggcacca acggcgacgg caaaaccgcg cgagtgatgg
     6301 tgtcgccggc atcgcgccgc cgggccgccg aggccggtct cgatctatct accgtcgcgg
     6361 gcaccgggcc cgcgggtcgc atcgagccgg gcgacatcga ttccgcgctg gccgcgggcg
     6421 ccgcgcgtgg cgcgccgcca tccatcaatg cgcgcgccaa tgcgccgcac gcgccgcgca
     6481 acggcactga agaagtgaaa attatcggcc tgcggcgcgt gatcgccgag cgcctcaccg
     6541 aatccgcgcg cagcattccg caatattcct acgtggaaga atgcgacctc acacgcctcg
     6601 aagggttgcg caagcacctc aacgatcgtc gcccgccatc cgcgccgccg ctcaccttct
     6661 tgccgttcat cgtcgcggct ctcgctcggg tcctggacaa atttccacag tgcaatgcgc
     6721 tgtacgacaa ggaacgcggc gtgctcgtga agcacaaggc cgtgcacgtg ggcgtcgcca
     6781 ctcagacgcc gcaggggctc aaggtgccgg tcgtgcgcaa tgccgagtcg cgcgcgctgc
     6841 acgatctcgc cgccgaaatc cggcgcgtcg ccgaggcagc gcgcaccaac aaatcgccgc
     6901 gcgaggagct caccggctcc accatcacga taaccagtct cggcaagttg ggcggcatcg
     6961 cctcgacacc gatgatcaac atgcccgagg tgtccatcat cggcatcaac aaggccatcg
     7021 agcgcgccgt ggtggtcgac gggcaggtca cgatccggct catgatgaac ctgtcgtcgt
     7081 cattcgatca tcgcttcgtc gacggcttcg atgcggcctc gatgatccag gcactcaagg
     7141 actcgctcga gcagccggcg acgatcttc
//
LOCUS       DASQJS010000234        19640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3905324, whole genome shotgun sequence.
ACCESSION   DASQJS010000234 DASQJS010000000
VERSION     DASQJS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19640
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3905324"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..951)
                     /locus_tag="VEW08_09415"
     CDS             complement(103..951)
                     /locus_tag="VEW08_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYJ40997.1"
                     /translation="MKSGKQTQLHMWTPDGLPGVTLFRAEHFQQRFARHTHDEYALGV
                     ITSGVLGFDYRGAHHLAGRGEINLVVPGEVHTGQPEQGDHWSYRMFYLEPALVASIAQ
                     QAGRGRGDPFFPAGVLRDESLAASVVQLHRDLDARLVDALEAESRLLAVLTTWLQRHG
                     EKQRCGRTGAHAQAVARVREFLDDCGSQNPTLTGLARLVELSPYQLLRAFMREYGLPP
                     HAWLIQRRVREARALLDRGLSVVDAAASTGFADQSHLNRHFKRILGFTPGRYCNFVQE
                     RRNAHH"
     gene            complement(1030..1839)
                     /locus_tag="VEW08_09420"
     CDS             complement(1030..1839)
                     /locus_tag="VEW08_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HYJ40998.1"
                     /translation="MTEAQTHAPIRTLIVDDETPARQWLRTLCARMADVRIIGECATA
                     AEASRTLRTTTVDLLLLDIQLGAHTGFQVLDGVAYSAVPNLVFVTAHDQYAVRAFDRR
                     AVDYLLKPVREDRFRESLERVRRQLRGGMTSEIQSAVRETIMPLEQSLLSLRSHGYTE
                     RLVGERDGAYRIIECHKMVSMESEGNYVRVAEEGVAEPAIMRGTLQGLAAILDPEQFV
                     RVSRFAILNLSFVERIERDADSHLVFILRDGRSFAVGRAYAADVHRALRMG"
     gene            complement(1836..2969)
                     /locus_tag="VEW08_09425"
     CDS             complement(1836..2969)
                     /locus_tag="VEW08_09425"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYJ40999.1"
                     /translation="MRSRDNTTDSPPALSLRATLTIATLFWIYSCLAVVLRLELTRQG
                     LSGKGPSIGVLTTCCLLLYPVLCALTYLSWRAGYDSARWARLVLVNFVLACVFGATSR
                     PAYVLASALHRDQTIAEANERINGKDFTKAVKLWGSSFVEDAALYLMLQAMLFGASAF
                     VRLKDESAARDRLAHEFDRARLQALRMQTNPHFLFNTLTAIAGLINARPAAAQSMVTQ
                     LGELFRATLEERDAELVSLQRELELGRQYLEIQRTRFDSRFDYSFMIQPEAEQAAIPP
                     LLLQPLLENAAEHGLTTQEGSVLVEVDATCASDEVHIVVRNRASVGKPRKRGSHRGLG
                     LELVRERVRAAFGPMAGLQTELTDDGTFLARLTFPQREVPVIA"
     gene            complement(3310..3801)
                     /locus_tag="VEW08_09430"
     CDS             complement(3310..3801)
                     /locus_tag="VEW08_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HYJ41000.1"
                     /translation="MQDENFDETTIKDIISRMIDAWNHGNAAEFAAPFAEDADFIAFE
                     GTHLKGRQQIRGFHERLFDLDLKGTRLEGGVKFVRFLGPALAVMHAWCTTTLKGQTNA
                     SSSRDSMQLFVVTKRDGQWRCDAMLNARRITMEQQLFQDQFETLSPHDQREVTHRLSA
                     MRH"
     gene            complement(3904..4371)
                     /locus_tag="VEW08_09435"
     CDS             complement(3904..4371)
                     /locus_tag="VEW08_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41001.1"
                     /translation="MEIDTSDWQEESKIEGEDPEDTELLREMAAEARDYIEDFEWAPR
                     ITSIHLAFGIGGVVAAFLFQFDEVIEDSDDALWVIVGDLPSAYVIVEPEDDGMSALSR
                     YCEMMEDWTINVLEGNSLDDSFPVDVEATAEHAEMLNQRIAFLRTEIIESPED"
     gene            complement(4403..5500)
                     /locus_tag="VEW08_09440"
     CDS             complement(4403..5500)
                     /locus_tag="VEW08_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ41002.1"
                     /translation="MQFKRRDALKVLGGATLATLVDPAFARAKPLKVLILGGTGFIGP
                     HFVDVLMAGGHKVALFNRGKRDPEVREGVEQLLGDRDGQVDSLKGRDWDVVIDNSGYK
                     PSQVRLTAEMLEEHVKHYIFISSIAVYTNFAKAGVDEDYELASLKDPTNETVTGETYG
                     GLKVLCEKIVETVYKDRSTIIRPTYIAGPGDHTDRFTYWPWRVSKGGEMLAPGTPTDP
                     FQYIDVRDLADFIRTCVEKTVTGRYNLCGPQGAVTMGSLLEESKRITKANTTFVWASP
                     EFLTAQEIIGEKAKGNLMPIWQPPVGDEAGLLLVSPARARKKGLKFRSLETTIRDTLE
                     WQKTRSEDKQVLKAGLTLEKEKELLGKLREA"
     gene            complement(5585..5794)
                     /locus_tag="VEW08_09445"
     CDS             complement(5585..5794)
                     /locus_tag="VEW08_09445"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HYJ41003.1"
                     /translation="MSTERVVVAFSGLAILLSLVLAYFVSPNWLAFAAIIGLNLSQSA
                     FTGFCPFEYVLKKFGMAGRDAPNPR"
     gene            complement(5846..6943)
                     /locus_tag="VEW08_09450"
     CDS             complement(5846..6943)
                     /locus_tag="VEW08_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ41004.1"
                     /translation="MQIKRRDALKVLGGATLASLADPVFAKSKPLKVLILGGTGFIGP
                     HFVTAIKGGGHSLTLFNRGKRDPEADPGIEQLIGDRNGQIDALKGRDWDAVIDNSAYT
                     PKQVKLTAELLKGHVKQYVLISSVAAYANFAKAGIDESYELMKLDDPTVEEVNGQTYG
                     GLKALCEQVAVGVFGKQATLIRPTYIAGPGDYTDRFTYWPVRVSKGGEMLVPGAPSDP
                     FQIIDVRDLADFMRVVVEKNVTGAFNLCAPPRAVTMGSLLDTCKRVTGADTRFTWATP
                     EFLEAQGLIGKDLFAPSGFPLWTPTNSSYSGMPLISPARAVKKGLKFRSLEATVRDTL
                     AWQSTRPAENQTLRTGLKPEQEAEVLAKLRA"
     gene            7099..7506
                     /locus_tag="VEW08_09455"
     CDS             7099..7506
                     /locus_tag="VEW08_09455"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYJ41005.1"
                     /translation="MASIRKEIRIDAPVDRVWEALRDVGALHTKLVPGFVKDTHMEGN
                     TRIVTFGNGMTAREEIVSVDPAQHRVAWAIVGQQFHHYNGAAWVELDPKGGSRFIWTT
                     DLLPNELAGQVDQMMTVGIAVVKQTLEASAQAA"
     gene            complement(7507..8004)
                     /locus_tag="VEW08_09460"
     CDS             complement(7507..8004)
                     /locus_tag="VEW08_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41006.1"
                     /translation="MRKFGQLGWGIALAFAIAAIVAGVWFAFLSWKLASPMLGILSVV
                     LVLAGPALTVALIVARPDAPAKAGEPPAGVLVDRIRRTESALRIAGLGRAHLGVIASY
                     VVIMWICEFAGMVSLKGLLVFLTVACAVTAMAYLPWLASRERRLYEERAEFQRRLGEI
                     EAGRL"
     gene            8406..9161
                     /locus_tag="VEW08_09465"
     CDS             8406..9161
                     /locus_tag="VEW08_09465"
                     /inference="COORDINATES: protein motif:HMM:NF025175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HYJ41007.1"
                     /translation="MPFTISHAAAVIPLRGFARFPLPLAALMIGSLSPDFAYFLPGEL
                     GRVETHSFAGLFWFCWPVSIGLWLLFVRVLELPTIALLPENWRTRFAPSNQEINLHVL
                     AFASAAVILGAVTHVVWDSFTHRGTVAVNAIPALKAVAFRVEGWRIRWFFVLQVLSSV
                     FGLLVLAFWAWRQAPGQYPRPSTPSVTHATRMRALAVLIAASLGLAITSYYFHSHMWL
                     TARVFHFLIGGMTGLALAWCAIAVLIRRSWKAA"
     gene            complement(9166..10374)
                     /locus_tag="VEW08_09470"
     CDS             complement(9166..10374)
                     /locus_tag="VEW08_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41008.1"
                     /translation="MSVPDTIAVQAPAASAVQRPSAWVRGPVWDGVWMLNALWLVPIA
                     LWLAHGYSDPEASPLDAFYFGLTALFWIGHRLGSTWLAYCTEAYRPLLRSEPVRFVLL
                     PLLVTGACFAIFLPPDDALPWTRGERFVGMAILDYALVTYHFAAQHFGALSLYRTRAE
                     RARCAQTRRHDRIFALGVGGALIFLADILAGAVAYPDLWINRWLIPGWVASNQDAIRE
                     AAMLVLFVATSFMLIAEARAPRRSLPRVLYILGLAAMVTIALQPRSLFLYVAIWTAQH
                     WILATGLASQTPGGEPAPASGNVRRVLHALNVRPWAVLLVLGTASVLLLPLFEVEANL
                     DLTGGAFYGDRIFGAAAEQLRTSSWVPGIVAIAFATGFVHYLLDRNVFRMSDPRVRAA
                     ARGLLDPPRM"
     gene            complement(10389..11024)
                     /gene="msrA"
                     /locus_tag="VEW08_09475"
     CDS             complement(10389..11024)
                     /gene="msrA"
                     /locus_tag="VEW08_09475"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HYJ41009.1"
                     /translation="MALLSKKTTIPAESDALPGRNTPLPLSGKHFVNGSNIKQPATGG
                     LEEAVFGLGCFWGAERKFWQIPGVVTTAVGYAGGYTPNATYEEVCSGRTGHTEVIRVV
                     YDPNKVSYETLLKAFWESHDPTQGMRQGGDVGTQYRSAIYTTTDAQQKAAEASKRSYQ
                     EALTKAHRGTITTEIRDAPEFFYAEEYHQQYLAKNPGGYCGLGGCGVAFNA"
     gene            11106..11690
                     /locus_tag="VEW08_09480"
     CDS             11106..11690
                     /locus_tag="VEW08_09480"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HYJ41010.1"
                     /translation="MTESASTPRLRATFAIYIALIAATAIVGPREIPLGWHYLAGLAG
                     FIFVALACLGRIWCSLFIAGHKDEVLVTNGPYALCRHPLYSFSVLGALGLGLTSRSAL
                     LCVAVVVLIMALVVYAAACEEQFLADAFPDEFKAYLVATPNKWLPKSARASLPAVLDV
                     RPAVFWKAFLDAGSFFVLWVLVALAAEYRVIPTF"
     gene            11741..12196
                     /locus_tag="VEW08_09485"
     CDS             11741..12196
                     /locus_tag="VEW08_09485"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HYJ41011.1"
                     /translation="MKTSVVKILVLLLCAIPAAAFLTTSCATSPDSVRAASTLPLLNT
                     SWRLTQLGDEVIDNPAGERAVNFLLQPSSTNLVGFSGCNRMFGRYALEGPSLKFDGLG
                     GTRMFCEAQMPLEQRFLAMFSQVSGWEISGSTLRLLDANGKTAATFNTP"
     gene            12193..12927
                     /locus_tag="VEW08_09490"
     CDS             12193..12927
                     /locus_tag="VEW08_09490"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HYJ41012.1"
                     /translation="MRALAAIALSMLLAACGGPAPTPAPTVSNAPSPPEKQMRGMYSY
                     MADAGWFTDCLSGMRMPVAQEGDNATLEAAYSKARQMAGAPMLAVIEGRVENRLPMEG
                     NTQRPTLIVEKFVLVESQGCSGPQSTAQLENTYWKLMTLDGASMESPEGAREIHFVMH
                     SEGARVAGFAGCNQMMGTYQVTGAKISFSQMGGTLMACAHGMEQEQKFHQLFPRVAGW
                     KIAGETLQLLDGAGAVLATFESRYMK"
     gene            13035..14369
                     /locus_tag="VEW08_09495"
     CDS             13035..14369
                     /locus_tag="VEW08_09495"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ41013.1"
                     /translation="MAAGTPAVPVVIEHVTVLPMTTDGEPVRDATVTLRDGRITSIET
                     GRISAPRGATRINGRGKFLLPGFTDMHMHLENDRGMRIATGEKSIPYGLIQLEDVLMP
                     YLANGVTQVFNLTAMSDTIGQRVEIESGRVLGPHIATAALIDGSPPMWPLGISRAAAT
                     PDDGRQAVRDAWAEGYDFIKVYSKLSLETFTAIVDEARKHKMRVVGHIPGREMNITGQ
                     FFQPGFDLVAHAEEFAQHTSPPSHEAIPRYVEMAKSNGTWLTATLTLDERLVEEIRDP
                     SSLTRRPELLYLPPHGYRDVIHDNRYLKDRSEKRIAYIQAIVEFNRVLVKAFASAGVP
                     VLTGTDAPVPGVMPGFSLHDEFQALAKAGMSNRQVLDGTTRLAAEWLGTIDDRGTVEA
                     GKRADLVLLDANPLDDIANTRRIAAVFVNGRYLPRADLEKRMAALAAKNQPQ"
     gene            complement(14410..14709)
                     /gene="yecR"
                     /locus_tag="VEW08_09500"
     CDS             complement(14410..14709)
                     /gene="yecR"
                     /locus_tag="VEW08_09500"
                     /inference="COORDINATES: protein motif:HMM:NF025358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR family lipoprotein"
                     /protein_id="HYJ41014.1"
                     /translation="MNRLLLIAILALAAGCTTYKLWNEAGSDQDTATVDLSYEFRKYE
                     SPQVDERAGQQMARERCKDWGYTNAHRKGEDRECLDGTTESCNKWRVTRQYRCTK"
     gene            14807..16738
                     /locus_tag="VEW08_09505"
     CDS             14807..16738
                     /locus_tag="VEW08_09505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ41015.1"
                     /translation="MELPEQVPGAEASQFKLLGKRRFLPFFLTQFLGAFNDNLFRNAV
                     VVSLTFGVAASAEAGVLANVSQGLFILPFFLFSALSGQLADKYEKSRLIRQTRFAEVV
                     LMCCGAAALYIGQVSILLGVIFLLGVLATIFGPLKYSLMPQHLRQSELVGGNALVDAG
                     TFIAILVGTIVGGLLAPTSTTAVESGGDAHLAASVTMVAVAVLMYLCSRAIPRAEATD
                     PGLKVNFNPITSTWEVIRFAAKTRAIFLSLLGISWFWLVGALILAQLPAYARDVVGGD
                     KTVYTLLLAAFSIGTALGSLACERLSGHKVEIGLVPLGSVGMTVCLLDLYFEHPGVHS
                     AGAAIISWKEFLSAGGWDIALDCALIGVFGGLFIVPLYALILQRSTESHRARIIACNN
                     IMNAGFMVIAALLAIVWLEVLHFTIPQLFILAAVLNAIVATYIYSLVPEFLMRFLSWV
                     LVNILYRIKVRGLENIPETGPVLIVCNHVSFMDPLVIGGSVRRPVRFVMDHNIFKIPV
                     MSFIFRTARAIPIAPGHEDPAALQRAFDQIDAELADGEIVCIFPEGKLTKHGELNEFK
                     KGVEKILERRPVPVVPMALRGLYGSFFSRQDGKAPMTKPPRRFWSRIEVVVTAPVPGD
                     DASATGLQKIVAGLRGEWQ"
     gene            16769..17404
                     /locus_tag="VEW08_09510"
     CDS             16769..17404
                     /locus_tag="VEW08_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41016.1"
                     /translation="MPGTILKSDVFGRIRRVGDVVVREADLARPWARALALHLMRREH
                     RALSRLALGAGIEGVPRILDLEAMRLTRSWIDGAPMQIAKPRDPAYFRAALRLLRRLH
                     AANVIHNDLAKETNWLVTPDGLPALVDFQLAMTLTKRGALARALGHDDLRHLLKHKRS
                     YLPDRLTAREKRILARHSLISRIWMASGKKVYLFVTRRILRWRDREGAGDR"
     gene            complement(17418..17840)
                     /locus_tag="VEW08_09515"
     CDS             complement(17418..17840)
                     /locus_tag="VEW08_09515"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HYJ41017.1"
                     /translation="MAENKTKPTKVSVAAFLKAKTSGQQLEDSQELVKLFKELTGKPA
                     KMWGPSIVGFDSYHYVYESGREGDAPVIGFSPRKPELVLYLAPYLNDKGLKARLGKHK
                     AGAGCLYVKTLDDIDREVLKALAKTSIAELRKRYPPQR"
     gene            complement(17789..18298)
                     /locus_tag="VEW08_09520"
     CDS             complement(17789..18298)
                     /locus_tag="VEW08_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HYJ41018.1"
                     /translation="MPITPFHFGPGVLFHAAAPRRVSFIAFMAANCIADCESIYNILR
                     GNFPLHRFLHTFVGAALVAAMTIALFMAMRRLARRTPVPNWFDWQQLTLSPVIVGALL
                     GSYSHIVLDGIMHADVRPFAPWSDANPFLHAVSLGTLHLACVILGIAGVLLWLRTRPS
                     RPKSPSPLS"
     gene            complement(18357..18827)
                     /locus_tag="VEW08_09525"
     CDS             complement(18357..18827)
                     /locus_tag="VEW08_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ41019.1"
                     /translation="MTKKRASITVRPVSPEDHDAWRPLWDEYNTFYERTGPTALSEEL
                     NDTLWRRFFDASEPVHCIVAEREHKVVGMCHFIYHRSTSRMELLCYLQDLFTAPDYRG
                     HGIGRALIERVYEIAKDAGCKRVYWQTHTTNTPGRTLYDKVAKHFGFIVYAKDV"
     gene            complement(18824..19390)
                     /locus_tag="VEW08_09530"
     CDS             complement(18824..19390)
                     /locus_tag="VEW08_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41020.1"
                     /translation="MRNVSILAMGLLLAASASAQAPASKAEWDAYRSQMVKRMRIVDR
                     AYELRPKRRDEPLRYLNISDYEVREIQLVAEKYLPQVLLNISPVVTGCPCEEGPQCTD
                     QVYIVAETPQSSKGLQLSRIRNAWVVGSVQQWWLKRDALAANSGKMSYAKYESAVNEL
                     VRDFPMCVGELVPAENSTASTPKAEPKK"
BASE COUNT         3588 a         6215 c         6045 g         3792 t
ORIGIN      
        1 gaagtccgag agaattcctg cctgggcgtt gagccgttcg ctctcttccg cgctgtatcc
       61 gtgaacgtat acaccagcca tttccacctc ctggagggga atctagtggt gtgcgttgcg
      121 gcgttcttga acgaaattgc agtagcgtcc cggcgtgaat cccaggatgc gcttgaaatg
      181 tcgattcaga tggctctggt cggcgaagcc cgtcgacgcg gcggcgtcca ccactgacaa
      241 gccgcggtcg agcagcgcgc gcgcctcgcg cacgcgccgc tgaatcagcc aggcgtgcgg
      301 tggcaatcca tactcgcgca tgaacgcgcg cagcagttga taggggctca gttccacgag
      361 gcgtgcgagc ccggtgagcg ttgggttctg gctgccgcag tcatcgagaa actcccgaac
      421 cctcgcgacg gcctgcgcat gcgcgccagt cctcccgcat cgctgcttct cgccgtgacg
      481 ctgcaaccat gtggtcaaca ccgccagcaa ccgtgactcg gcctcgagcg catcgacgag
      541 ccgcgcatcg agatcgcgat ggagctgaac gaccgacgcc gccaggctct catcgcgaag
      601 cacacccgcg ggaaagaacg gatcaccacg gccgcggccc gcctgctgcg cgatgctcgc
      661 caccagcgcc ggctctagat agaacatgcg gtaactccag tgatcgccct gttccggctg
      721 accggtatgc acctcgccgg gaaccacgag attgatttcg ccacgaccgg ccaggtgatg
      781 cgcgccacgg tagtcaaacc cgagcacgcc gctggtgatg acgccgagcg cgtattcatc
      841 atgtgtgtgg cgggcgaagc gctgctggaa atgttcagcg cgaaacagcg tgacgccggg
      901 caagccgtcc ggcgtccaca tgtgaagctg cgtctgtttg ccggatttca tggtcacgtt
      961 ctgtattcaa tcaggtcggg gaactggcag ctatcacttc aatgcctcgg cgcggcccga
     1021 cggccgcgtt tatcccattc gcagcgcgcg gtgaacatcc gcggcgtagg cacggccaac
     1081 cgcaaaactg cgcccgtcgc gcagaatgaa caccaggtgg ctgtcggcgt cgcgctcgat
     1141 gcgttcgacg aaactcaagt tgagaatggc gaagcggctg acgcgcacga actgttcggg
     1201 atcgagaatg gccgcgagcc cttgcaaggt gccgcgcatg atcgcgggtt cagcgacgcc
     1261 ctcttccgcg acgcgcacgt agttgccttc ggattccatg ctcaccatct tgtggcactc
     1321 gatgatgcga taggcgccat cgcgctcgcc caccagccgc tccgtgtagc cgtgcgagcg
     1381 cagcgacagc agcgattgct ccagcggcat gatggtttcg cgcaccgccg actggatctc
     1441 actggtcatg ccaccgcgaa gctggcggcg cacgcgctcc agcgactcgc ggaagcggtc
     1501 ctcgcgcacg ggtttcagca ggtaatccac cgcgcgccga tcgaacgccc gcaccgcgta
     1561 ctggtcgtgc gcggtcacga acaccaggtt gggtacggcc gagtacgcca cgccgtcgag
     1621 cacctggaac ccggtatgcg caccgagctg gatgtccagc agaagcagat cgacagtagt
     1681 cgtgcgcagc gtgcgcgagg cttcggcggc cgtggcgcac tcgccgatga ttcgcacgtc
     1741 cgccatcctg gcgcacagag tgcgcagcca ttggcgagcc ggtgtttcgt cgtcgacgat
     1801 caaggtgcga atgggtgcgt gagtctgcgc ttcggtcatg cgatcaccgg aacttcacgt
     1861 tggggaaacg tgaggcgcgc caggaaggtg ccgtcgtcgg tgagttccgt ctgcaggccg
     1921 gccatgggac caaacgccgc ccgaacccgc tcgcgcacca gctcgagtcc gagtccgcga
     1981 tgcgaacccc gcttgcgagg tttgccgacc gatgcccggt tgcgaacgac aatgtgcact
     2041 tcatccgacg cacacgtagc gtccacttcc accagcaccg agccctcctg ggtcgtgagc
     2101 ccatgctcgg cggcgttctc gagcagcggt tgcagcagca gcggcggaat cgcggcctgc
     2161 tccgcttccg gctggatcat gaaggagtag tcgaatcgcg aatcgaaacg cgtgcgctga
     2221 atctccaggt actgacggcc gagctcgagc tcgcgttgca aagacaccag ctccgcgtcg
     2281 cgctcttcga gcgtggcgcg aaacagctcg cccaactgcg tcaccatgga ctgggccgcc
     2341 gccggccgcg cgttgatgag tccggcgatg gcggtcagcg tgttgaagag aaagtgcggg
     2401 ttcgtctgca tgcgcagcgc ctggagccgc gcgcgatcaa attcatgcgc gaggcgatcc
     2461 cgcgccgcag actcgtcctt gagccgcacg aacgcgctgg cgccaaacaa catggcctgg
     2521 agcatgagat agagcgcggc gtcttcgacg aaagacgacc cccacagctt caccgccttg
     2581 gtgaagtcct tcccgttgat gcgttcattg gcctcggcga tcgtctggtc gcggtgcaag
     2641 gcactggcga gaacgtacgc ggggcgcgac gtcgcaccaa acacgcaggc gagcacgaag
     2701 ttcacaagta ccaatcgcgc ccaacgcgcc gaatcgtagc cggcgcgcca tgacaggtat
     2761 gtgagcgcgc acagcaccgg atacagcagc aggcaacaag tggtgagcac gccgatggac
     2821 ggccccttgc cgctcagtcc ctggcgcgtg agctcgagac gcaaaaccac cgcgagacac
     2881 gagtagatcc agaagagcgt cgcgatagtt agggtagccc gcagcgacaa ggctggtggc
     2941 gaatcagtgg tgttgtcgcg acttctcaag atgagcgaat tctatcggca ttctccgcat
     3001 gcgggtcgcg cgtggccgcg tgatgaacga ccgccagcgg ggaccaacaa ccgcccgctg
     3061 gcgcaggcgc atggggcggc atagaaggac cacggcgatg gcgctgtcga aggctggccc
     3121 ggcttcgggc cgtttccccc aacgtctaac atccttcgaa tggcggcaaa gccaacatcg
     3181 accagtgcga gcctggtggc aatgctctcg cgccacgaac gctcgcgctg gtcgatggtt
     3241 cccggaagcg cggaaggctc gttcacggcg tcccgtcgaa agacaggcgc cgtgaacact
     3301 tatgccaatt cagtggcgca tcgccgaaag gcggtgcgtg acctcgcgtt gatcatgagg
     3361 cgacagcgtc tcgaactgat cctgaaagag ttgctgctcc atcgtgatcc ggcgcgcgtt
     3421 gagcatcgcg tcgcagcgcc attgcccgtc gcgcttcgtc accacgaaca gttgcatcga
     3481 gtcacggctc gacgatgcgt tcgtctggcc cttcagcgtc gtcgtacacc aggcatgcat
     3541 gaccgcgagc gcgggcccca ggaaacgcac gaatttcaca ccaccctcga gccgcgtgcc
     3601 cttgagatcg agatcgaaca gccgctcgtg gaagccgcgg atctgctgcc ggcccttgag
     3661 atgtgtgcct tcgaaggcga tgaagtccgc gtcctcggcg aacggcgccg cgaattccgc
     3721 cgcgttgccg tggttccagg catcgatcat ccgcgagatg atgtccttga tcgtggtttc
     3781 gtcgaaattc tcgtcctgca tcgagttctc ctcattccgt ttacggtgtg agggaatgca
     3841 gctcgaggag ttccaggaaa tgcgtcgaga gaaattgtcg gcgtcagtct gacgtcgcgc
     3901 gaatcagtcc tccggagact cgatgatctc cgtgcgaagg aatgcgattc tctggttgag
     3961 catctccgca tgttcagcgg tcgcctcaac atcgacaggg aaggaatcgt cgagcgagtt
     4021 gccttccagc acattgattg tccagtcttc catcatctcg caataccggc tcaacgccga
     4081 catcccatcg tcttcaggct cgacaatgac gtaggcactc ggcaggtctc cgacgatgac
     4141 ccagagcgcg tcatccgagt cttcgatcac ctcgtcgaat tggaaaagga aagcagcgac
     4201 gaccccaccg atgccaaatg caaggtggat gctggtaatg cgtggcgccc actcgaagtc
     4261 ctcgatgtag tcccgcgcct ctgccgccat ctcgcgcagc agttccgtgt cctcgggatc
     4321 ttctccctcg atcttggatt cttcttgcca gtcgctcgta tcgatttcca tgagtctcat
     4381 gccgcctgcg gagttgtgga cgctaagctt ctcgcaattt ccccagcaac tccttctctt
     4441 tctccagcgt cagccccgcc ttcagcacct gcttgtcctc cgaccgcgtc ttctgccact
     4501 ccagcgtgtc gcgaatggtg gtctccaggc tccggaactt gagccccttc ttcctcgccc
     4561 tcgccgggct caccaacaac aacccagcct catcgcccac cggtggctgc cagatgggca
     4621 tcaggttgcc cttggccttc tcgccgatga tctcctgagc ggtcaggaat tcggggctgg
     4681 cccacacgaa cgtggtgttg gccttggtga tgcgcttgct ctcctcgagc agcgatccca
     4741 tggtgacggc gccctgcggg ccgcagaggt tgtacctgcc ggtcacggtc ttctcgacgc
     4801 aggtgcggat gaagtcggcg aggtcgcgca cgtcgatgta ctggaacgga tcggtgggtg
     4861 tgccgggcgc gagcatctcg ccgcccttgg acacgcgcca tggccagtag gtaaatcgat
     4921 cggtgtggtc accgggcccg gcgatgtagg taggccggat gatggtgctg cgatccttgt
     4981 acacggtctc gacgatcttc tcgcacaaca ccttgaggcc gccgtaagtt tcgccggtca
     5041 cggtctcgtt ggtcggatcc ttgagcgagg cgagctcgta gtcttcgtcg acgccggcct
     5101 tggcgaagtt cgtatagaca gcgatgcttg aaatgaagat gtagtgcttg acgtgctctt
     5161 cgagcatttc ggctgtgagc cgcacctggc tgggcttgta gccggaattg tcgatgacga
     5221 cgtcccagtc acggcccttg agcgagtcga cctgtccgtc gcgatcgcct aacagctgct
     5281 cgacgccttc gcgcacttcc ggatcgcgct tgccgcgatt gaacaacgcg accttgtggc
     5341 ctccggccat cagcacatcg acgaagtgcg ggccgatgaa gccggtaccg ccgaggatga
     5401 gcaccttgag gggcttcgcc ctggcaaagg cgggatccac cagcgtggcg agcgtggcgc
     5461 cgccaaggac cttcagtgca tcgcgtcgtt tgaactgcat gaccactacc tcggagttgt
     5521 tggccctgag gatagcggac aaggtggcgg cacgcctcac ggcgatccgg cccatcgctt
     5581 cgcgtcagcg cggatttggc gcgtctcgtc ccgccatgcc gaatttcttc aacacatact
     5641 cgaacgggca gaaacccgtg aacgccgact gcgagagatt gagtccgatg atggccgcga
     5701 atgccagcca attcgggctc acgaaatacg cgagcaccag gctcagcagg atcgcgagac
     5761 ccgagaaagc aacgacgacg cgttctgtgg acatgccaca ttcatgcaca tcgcgcaccc
     5821 gcgcgaaaat cagggctgct acgtatcagg cgcgcagttt cgccagcacc tcggcttcct
     5881 gctccggctt caaccccgtt cgcagcgtct gattctccgc tggtcgtgtc gactgccacg
     5941 ccagcgtgtc gcgcacagtg gcctcgagcg atcggaactt cagtcccttc ttgaccgcgc
     6001 gcgcggggct gatcagcggc atgcccgagt acgagctgtt cgtcggggtc cacaacggaa
     6061 acccggaagg cgcaaacaga tccttgccga tgagtccctg ggcctccagg aactcaggcg
     6121 ttgcccaggt gaaccgggtg tcggcgccgg tcacacgctt gcaggtgtcc agcagtgatc
     6181 ccatggtgac ggcgcgcggc ggcgcgcaaa ggttgaacgc gccggtgacg ttcttctcga
     6241 ccacgactcg catgaaatcg gcgaggtcac gcacgtcgat gatctgaaac ggatcgctgg
     6301 gtgcgccggg cacgagcatc tcgccaccct tcgacacgcg cactggccag tacgtgaacc
     6361 gatccgtgta gtcgccaggc cccgcgatgt acgtgggccg gatcaacgtg gcttgtttgc
     6421 cgaagacccc cacagccacc tgctcacaca gcgccttcag gccgccgtaa gtctggccgt
     6481 tcacttcctc gacggtgggg tcgtcgagct tcatgagttc gtagctctcg tcgatgccgg
     6541 ccttcgcgaa gttcgcatag gcggcaacgc tcgagatcag cacgtattgc ttcacgtggc
     6601 ccttgagcag ttcggccgtg agcttcacct gcttcggggt gtaggcggag ttgtcgatga
     6661 ccgcatccca gtcacgccct ttcagcgcgt cgatctgtcc gttgcgatca ccgatcaatt
     6721 gttcgatgcc gggatcggct tcgggatcgc gcttgccgcg attgaacagc gtcagcgagt
     6781 gaccgccgcc cttgatcgca gtgacgaagt gcggaccgat gaacccggtg ccgcccagga
     6841 tcagaacttt gagtggcttt gacttggcga agacgggatc ggcgagcgag gcgagcgtgg
     6901 cgccgccgag aaccttcagt gcatcgcgtc gtttgatctg catggccgcc acctcgggat
     6961 tgttggaccc gaggatagcg aacctcgggc cgggttcggg acaatttacg cggcagattc
     7021 gggcggccga tgtggttccc ggctgacggt gcctgggggc aagccggtca gaatgggtga
     7081 aacattggag gcaacaatat ggccagcatt cgcaaggaaa tccgcatcga tgcccccgta
     7141 gatcgggtct gggaagcgct gcgcgacgtg ggcgctttgc acacgaaact ggtgcccggt
     7201 ttcgtcaagg acacgcatat ggaaggcaac acgcgaatcg tcaccttcgg caacggcatg
     7261 acagcgcgcg aagagatcgt cagcgtcgac cctgcccagc atcgcgtggc gtgggcgatc
     7321 gtgggccagc agttccatca ctacaacgga gcggcctggg tggagctgga tccgaaaggc
     7381 ggctcgcgtt tcatctggac cacggacctg ctgccgaatg agctcgcggg ccaggtggat
     7441 cagatgatga ccgtcggaat cgcggtggtg aaacagaccc tggaagcatc ggcgcaggcg
     7501 gcttgactac aaacgcccgg cctcgatttc tcccagccgc cgctgaaact ccgcacgctc
     7561 ttcgtacagc cggcgctcgc gagatgcaag ccacggtaga taggccatcg ccgtcaccgc
     7621 gcaggccacg gtcagaaaca ccagcaagcc cttcagactg accattcctg caaactcgca
     7681 tatccacatg atgacgacgt atgacgcaat gacacccagg tgagcgcggc cgagcccggc
     7741 gatccgcaat gcggactcgg tgcggcgaat gcgatcgacc agcacacccg cgggcggctc
     7801 gccggccttg gccggggcgt ccgggcgtgc aacgatcagc gccaccgtga gcgccgggcc
     7861 cgccagcacc agtactaccg agagtatccc cagcattggc gatgcgagtt tccaggaaag
     7921 gaacgcgaac cagacgcccg cgacgatggc tgctattgcg aaggcgagcg cgatgcccca
     7981 ccccagttgg ccgaattttc gcatgtgatt cctggtcttc gtggacaaga aaatcttcca
     8041 gggcaatgca aatacaacgt aaccaccctt ccccaggggt tccgccgagg cccagggtgc
     8101 gctcttcgac ctcggtccga ttgcgcaatt tcgatggcct gtgttaatca gtcttcgccc
     8161 gaacgcggat cgggcatgcg cgctctgact gaaggggatc cagaattgga tagcagaagt
     8221 cccgcgccgc cggcctgacg cccagtcggg tacggtttcg ttcagcgcca gccctcgctc
     8281 gcattcctgc gcgcgaggag ctgcgcgcgg caatcacgat ggagtggtta gccgcagcgc
     8341 tgacaattca agcgccgcca caattcgtca ccttctcggc gagaaccggc tgctacgatt
     8401 cgccggtgcc gtttacgatc tcacacgccg ccgcggtcat accgctgcgc ggtttcgcca
     8461 gattcccact gcccctcgcc gcgctgatga tcggcagcct gtcgccggac ttcgcgtatt
     8521 tcctgcccgg agagctcggc cgcgtcgaga cgcacagctt cgcgggtctg ttctggtttt
     8581 gctggccggt cagcatcggc ctgtggctgc tgttcgttcg cgtgctcgaa ctgcccacga
     8641 tcgcgctgct gcctgagaat tggcgcacgc gattcgcgcc gtccaaccag gagatcaacc
     8701 tccacgtgct ggcgttcgca tcggcggcgg tcatcctggg cgcggtaacg cacgtggtct
     8761 gggattcgtt cacgcaccgg ggcacggtgg cagtcaacgc catccccgct ttgaaggcgg
     8821 tggcgtttcg cgtcgaaggc tggcggatcc gctggttttt cgtgctgcag gtgttgagct
     8881 cggtgttcgg gctcctggtg ttggcgttct gggcatggcg acaagcgcca ggacaatacc
     8941 cgcgcccatc gacaccgtca gttacgcacg cgactcgcat gcgtgccctc gcggtgctca
     9001 tcgcggcgtc acttggactc gcaatcacga gctactactt tcattcccac atgtggctca
     9061 cagcgcgcgt gtttcatttc ctgatcggcg gaatgacagg cctggcgctg gcctggtgcg
     9121 caattgccgt attgatccgg cggagttgga aagcggcctg atggatcaca tgcgaggcgg
     9181 gtcgagcagg ccgcgcgccg cggcacgcac ccggggatcc gacatccgga atacgttgcg
     9241 gtccagcagg tagtgcacga aaccggtcgc gaatgcgatg gctactattc cgggaaccca
     9301 ggaagaagtg cgtaattgct ccgcggcggc accgaaaatc cggtcgccgt aaaaggcacc
     9361 ccccgtcagg tccaggttgg cttctacctc gaagagcggc agcagcagga ctgaagcagt
     9421 tccgagaacg agcaggacag cccagggccg gacattcaat gcatgcaaga cacgccggac
     9481 gttaccgctg gccggtgccg gctctccacc tggagtctgg gatgcgagac ccgttgccag
     9541 gatccaatgt tgtgcagtcc agattgcaac gtagagaaac aggctccgag gctgaagagc
     9601 aatggtgacc atcgccgcga gtcccaggat gtagagcact cgcggcagcg agcggcgcgg
     9661 tgcgcgcgcc tccgcaatca acatgaagga cgtcgcgacg aacaacacca gcatggccgc
     9721 ttcgcgaatt gcatcctggt tcgatgcaac ccagcccggg atgagccaac ggttgatcca
     9781 caggtccgga taggcaacag ctccggccag gatgtcagca aggaagatga gcgcaccgcc
     9841 cacacccagg gcgaagattc ggtcgtgacg tctggtttgc gcgcaacgcg cgcgttcggc
     9901 tcgtgtccgg taaagagaca gggcgccgaa gtgctgtgcc gcgaagtggt acgtcacgag
     9961 ggcatagtcg agtatcgcca tacccacgaa gcgctccccg cgggtccacg gcaatgcatc
    10021 gtcgggcggc aggaagattg cgaagcacgc cccggtaacc agcagcggga gcaggacgaa
    10081 ccggaccggc tcactacgga ggagcggccg atacgcttct gtgcagtagg caagccatgt
    10141 gctgcccaga cgatggccga tccagaataa tgcggtcagc ccaaagtaga aggcatcgag
    10201 cgggctcgcc tccggatcag aatatccatg cgccagccag agggcgatcg gtacgagcca
    10261 gagcgcattg agcatccaca cgccgtccca gaccgggccg cgcacccagg cagatgggcg
    10321 ctgcaccgca gaggcggccg gggcctggac ggcgatcgta tccggcacgc tcattgcgag
    10381 ccctccgatc aggcgttgaa ggcaaccccg cagccgccaa gcccgcagta gccacctgga
    10441 ttcttcgcca ggtactgctg gtggtattcc tcggcataga agaattccgg agcgtcgcgg
    10501 atctcggtgg tgatggtccc gcgatgcgcc ttggtcagcg cttcctggta cgagcgcttg
    10561 ctggcttcgg cggccttctg ttgcgcatcc gtggtcgtgt agatagccga gcgatactgc
    10621 gtacccacgt ccccgccctg gcgcatgccc tgtgtggggt cgtgcgattc ccagaaagcc
    10681 ttgagcagcg tttcgtagct gaccttgtta gggtcgtaga ccacgcgtat cacttcggtg
    10741 tggccggttc gacccgagca cacctcttca taggtggcgt tcggcgtata tccgccggca
    10801 taacccaccg cggtggtgac cacacccggg atttgccaga acttgcgttc cgcgccccag
    10861 aaacagccga ggccaaacac ggcttcttcg agccctccgg tggccggctg cttgatgttg
    10921 ctgccattca cgaagtgttt gcccgagagc ggcagcggcg tgttgcggcc cggcagcgcg
    10981 tcgctttcag cgggaatggt ggtcttcttg gaaaggagtg ccatgtgggg ctcctggtat
    11041 cgcgggattg gacccgaagt gtgggggctt gggggttggt tctcaactgc tagactgcgc
    11101 cgccgatgac agaatccgcc agcacaccgc gccttcgcgc caccttcgcg atctacattg
    11161 cgctgatcgc agcgacggcg atcgtaggcc cgcgcgaaat cccgttggga tggcactacc
    11221 tggcaggcct tgcgggtttc atattcgtgg cgctggcctg cctcggccgc atctggtgct
    11281 ccctcttcat cgccggccac aaagacgaag tgctggtcac caacgggcca tacgccctgt
    11341 gccgccaccc gttgtattca ttttcggtgc tgggcgcgct gggcctgggg ctgaccagcc
    11401 gctccgccct gctttgcgtc gcggtcgtgg tattgatcat ggcgctcgtg gtctatgccg
    11461 ctgcctgcga agaacagttt ctcgccgacg catttccgga tgagttcaag gcatatctgg
    11521 tcgcgacgcc caacaaatgg cttcccaagt ccgcgcgggc gtcgttgccc gccgtactcg
    11581 acgttcgtcc ggcggtgttc tggaaagcct ttctggatgc gggctcgttc ttcgtgttgt
    11641 gggtgctggt ggcgctggcg gcggaatacc gcgtcattcc cacgttctga cagcccttgt
    11701 tttgacagca ttgggcgtgg cgccggtgta ggctttcgcc atgaaaacat ccgtcgtcaa
    11761 gattcttgtc ctgctgctct gcgccatccc cgccgcagcc tttctaacta cttcctgcgc
    11821 cacctcaccc gattctgtga gagcagcctc gacactgccc ctgctcaaca catcgtggcg
    11881 gcttacacag ctgggtgacg aagtcatcga caatccggcc ggtgaacggg ccgtgaattt
    11941 cctgcttcag ccctcgagca ccaatctcgt cgggttctcc ggctgcaatc gcatgttcgg
    12001 ccgctatgcg ctcgagggcc cttcactaaa atttgatggc ctcggcggca cacggatgtt
    12061 ctgcgaagca cagatgcctc tcgagcaacg ctttctggcg atgttctcgc aggtatccgg
    12121 ctgggaaatc agcggttcca cgctgcgcct gctcgatgcc aacggcaaga ctgcggcaac
    12181 gttcaacact ccatgagagc acttgccgca atcgcgttgt cgatgctgtt ggcggcgtgc
    12241 ggcggacctg cgccgacacc agcgccaacg gtgtcgaacg caccgagccc gccggaaaag
    12301 cagatgcggg gcatgtacag ctatatggcg gatgccggct ggttcacgga ttgcctctcg
    12361 ggcatgcgca tgcccgtggc gcaggaaggt gacaacgcca cgctcgaagc agcctattcg
    12421 aaagcaaggc agatggccgg cgcgccgatg ctcgccgtca tcgaaggccg cgtcgaaaat
    12481 cgccttccca tggaagggaa tacccagcgc cctacgttga tcgtggagaa gttcgttttg
    12541 gtcgagtcgc agggttgttc gggcccgcaa tccaccgcgc aactcgaaaa cacctactgg
    12601 aagctcatga cgctcgatgg cgcttcaatg gaatcgcctg aaggcgcgcg tgaaattcat
    12661 ttcgtaatgc attcggaagg cgcacgcgtc gccggattcg cgggctgcaa ccagatgatg
    12721 ggtacctacc aagtgacggg cgcgaagatc agtttcagcc agatgggcgg cacgttgatg
    12781 gcctgcgccc atggcatgga gcaggaacag aagtttcatc agctgtttcc gcgtgttgct
    12841 ggttggaaga ttgcgggcga gaccctgcaa ttgctcgatg gcgctggcgc agtgctcgcg
    12901 accttcgagt cgcgttacat gaaatagacc gctgcaaccc gccgggtgca tggcgggtat
    12961 caaccctgct tccaactcat ggtggggttt ccatgtcgcg cctgattgcc gctctttttt
    13021 tgttcacccc tgtcgtggcc gcggggacgc cagcggttcc cgtggtcatc gaacacgtca
    13081 ccgttctgcc aatgacgacc gacggcgagc cggttcgcga tgcgacggtg acgctgaggg
    13141 acgggcgcat tacatcaatc gaaactggca ggatatctgc gccgcgcgga gccacgcgca
    13201 tcaacggccg cggcaagttc ctgctacccg gcttcacgga catgcacatg cacctcgaga
    13261 acgaccgcgg catgcgtatc gccaccggcg aaaaatcgat tccctacggg ctcatccagc
    13321 ttgaagacgt gctgatgccc tacctcgcca acggtgtgac acaggtgttc aacctcaccg
    13381 ccatgtcgga cacgatcggg cagcgcgtcg agatcgagag tggccgggtg ttaggtccgc
    13441 atattgcgac ggccgcactc atcgatggct ctccgccgat gtggccgctc ggaatatccc
    13501 gcgctgccgc gacaccggac gatggccggc aggccgtacg ggacgcgtgg gccgagggtt
    13561 acgatttcat caaggtctac agcaaactca gcctggagac cttcaccgcg attgtcgacg
    13621 aggcgcgcaa acacaagatg cgcgtggtcg ggcacattcc cgggcgcgaa atgaacatta
    13681 ccgggcaatt cttccagccg ggattcgacc tggtcgcgca cgcggaagaa tttgcgcagc
    13741 acacgagccc cccatctcat gaggccatcc cgcgttacgt ggagatggcg aagagcaatg
    13801 gcacctggct tacggccaca ctcacgctcg atgagagact cgtcgaggag atccgtgatc
    13861 cctcgtcgct gacccggcgc ccggagcttc tctatctacc accacacgga tatcgggacg
    13921 tgatccacga caaccgatat ctgaaggaca gaagcgagaa gcgcatcgcc tacattcagg
    13981 cgatcgtaga attcaatcgc gtgctcgtga aggccttcgc gtctgccggt gttccggtgc
    14041 tcacgggcac cgatgcgcca gttcccggtg tgatgccggg gttttcgttg catgacgagt
    14101 tccaggcgct ggcaaaggca ggcatgagca accgccaggt gctcgacggc actacacgac
    14161 tggcggcgga gtggctaggc acgattgacg accggggcac cgtggaagcc ggcaagcgcg
    14221 ccgatctcgt gctgctggat gcgaacccgc tcgacgatat cgccaacaca cggcgcatcg
    14281 ccgcggtgtt cgtcaacgga cgttacctgc cgcgcgcgga tctggagaaa cgcatggccg
    14341 cgctcgcggc gaagaatcag ccgcagtgag gaggactggc cccttttgct gagaaaaggg
    14401 ggctgccccc tatttcgtgc agcgatactg acgcgtcacg cgccacttgt tgcaggactc
    14461 cgtggtgccg tcgaggcact cgcggtcttc gcctttgcga tgcgcgttgg tatagcccca
    14521 gtccttgcaa cgttcgcgcg ccatctgctg gcccgcacgt tcgtccacct gcgggctttc
    14581 gtacttccgg aattcatacg acagatcgac cgtggccgtg tcctggtcgc ttccggcttc
    14641 attccagagc ttgtaggtgg tgcacccggc tgccagcgcc aggatggcga tgagtagtaa
    14701 gcgattcatg tacccactgt agcgtttttt gctaccggac tggcagtggg cttctggtat
    14761 atatgcgcgc catgccaacg cattgttgcc ggccaggtag cgcctgatgg agcttccaga
    14821 acaggttccg ggcgcggaag cgtcgcaatt caagctgttg ggcaaacgcc gcttcctgcc
    14881 attcttcctg acgcagtttc tcggcgcctt caacgacaac ctgtttcgca atgcggtggt
    14941 ggtgtccctc accttcggcg tggcagcgtc agccgaggcg ggtgttctgg caaacgtctc
    15001 gcagggtttg ttcatcctgc cgttcttcct gttttccgcg ttgtccggcc agctcgcgga
    15061 caagtatgaa aagagccggc tgatacgcca gacccgtttc gcggaagtgg tgctgatgtg
    15121 ctgtggggcc gccgccctgt acatcggcca ggtctcgatc ctgctcggcg tgatcttcct
    15181 gttgggcgtg ctggccacca ttttcgggcc gctcaagtat tcgctcatgc cgcagcatct
    15241 gcgccagagt gagctggtgg gcggcaacgc gctggtcgat gccggcactt tcattgccat
    15301 tctcgtaggc accatcgttg gtggcctgct cgcgccgacc tcgaccacgg ctgtggagag
    15361 tggcggcgat gcccacctcg ccgcctcggt aaccatggtg gccgtggcgg tgctgatgta
    15421 cctgtgttcg cgcgcaatac cgcgcgcgga ggccaccgat cccggtctca aggtcaactt
    15481 caaccccatc acgtcgacct gggaggtgat ccgctttgcc gcgaagacgc gcgcgatctt
    15541 cctgtcgttg ttaggcattt cgtggttctg gctcgttggc gcgctgatcc tggcgcaact
    15601 gcccgcctac gcgcgtgacg tggtcggcgg cgacaagacg gtgtacacgc tgctgctcgc
    15661 cgcgttctcc atcggcaccg cgctcggttc gctggcttgc gaacggctct ccggccacaa
    15721 ggtggagatc ggtctcgtgc cgctcggatc cgtcggcatg acggtgtgcc tgctcgacct
    15781 ctattttgaa catcccggcg tgcattccgc gggcgcggcg atcatttcct ggaaggaatt
    15841 cctgtccgcg ggcggctggg acatcgcgct cgactgtgcg ttgatcggcg tgttcggcgg
    15901 cctgttcatc gtgccgctgt atgcgttgat cctgcaacgg agcaccgaga gccatcgcgc
    15961 gcgaatcatc gcctgcaaca acatcatgaa cgccgggttc atggtgatcg ccgcattgtt
    16021 ggccatcgtc tggctggaag tgctgcactt caccatcccg cagctcttca tcctggcggc
    16081 ggtgctgaac gccatcgtcg cgacgtacat ctactcgctg gtgcccgagt tcctgatgcg
    16141 cttcctgtcg tgggtgctcg tcaacatcct gtaccgcatc aaggtgcgcg gactcgagaa
    16201 cattccggaa accggccccg tgctcatcgt ctgcaatcat gtgagcttca tggatccgct
    16261 ggtcatcggc ggcagcgtgc gccggccggt gcggttcgtc atggatcaca acatcttcaa
    16321 gatcccggtc atgagcttca tctttcgcac ggcgcgcgcc attcccatcg cgccgggcca
    16381 cgaagatccg gcggcgttgc agagggcttt cgaccagatc gacgccgaac tcgccgacgg
    16441 cgaaatcgtc tgcatcttcc ccgagggcaa actcacgaag catggcgagc tcaacgagtt
    16501 caagaaaggc gtcgagaaga ttctcgagcg ccggcccgtg cccgtggtgc ccatggcgct
    16561 gcgcggcttg tatggcagct tcttcagccg ccaggatggc aaggcaccga tgaccaagcc
    16621 accacggcgc ttctggtcgc gcatcgaagt tgtcgtcacc gcacctgtgc cgggcgacga
    16681 tgccagcgcc acgggattgc agaagatcgt cgccggcctg cgcggcgagt ggcagtaaat
    16741 ggtgccaggc accgttttct atgaaacggt gcctggcacc attttgaagt ccgacgtgtt
    16801 cggccgaatt cgccgcgttg gcgatgtcgt cgttcgcgaa gccgacctcg cgcgcccctg
    16861 ggcgcgcgcg ctggcgctgc acctcatgcg ccgcgagcat cgcgcgctgt cgcgtcttgc
    16921 gctgggcgcc ggcatcgaag gcgtcccgcg catcctcgat ctcgaagcca tgcggctcac
    16981 gcgaagctgg atagacggcg cgcccatgca gatcgccaag ccacgcgatc ccgcctactt
    17041 ccgcgccgcg ctgcgcctgt tacgccgcct tcacgcagcc aacgtcatcc acaacgatct
    17101 cgccaaggaa accaactggc tggtgacacc cgatggcctc cctgccctcg tcgatttcca
    17161 gctcgcgatg accttgacga agcgcggcgc cctcgcccgc gcgctcgggc acgatgacct
    17221 gcgccacctg ctcaagcaca agcgcagcta tcttcccgac cgcctcaccg cgcgcgagaa
    17281 acgcatcctc gccagacatt ccctgatctc gcgcatctgg atggccagcg gcaagaaggt
    17341 ctatctgttc gtgacgcgca gaatcctgcg ctggcgcgat cgcgaaggcg cgggtgaccg
    17401 ctgatcacgt gccggcatca gcgctggggc ggatacctct ttcgcagctc cgcgatcgag
    17461 gtctttgcca gcgccttcag cacttcccga tcaatgtcat cgagcgtctt gacgtacagg
    17521 caacccgcgc ccgctttgtg cttgccgagc ctggccttca gtcccttgtc attgagatac
    17581 ggcgcgagat agagcacgag ctctggtttg cgcggcgaga agccgatcac cggcgcatcc
    17641 ccttcccgcc cgctttcgta cacgtagtga taagagtcga agcccacgat gctcgggccc
    17701 cacatcttcg ccggcttgcc cgtgagctcc ttgaacagct tcaccagctc ctggctgtcc
    17761 tccaattgct gcccggacgt cttcgctttc aggaaagcgg cgacggagac tttggtcggc
    17821 ttggtcttgt tctcagccat aacaacactc ccgcgattcc caggatgacg cacgccagat
    17881 gcaaggtgcc gagcgaaacg gcgtgaagga atggattcgc atcgctccac ggcgcgaacg
    17941 ggcgcacgtc ggcatgcatg atgccgtcga gaacgatgtg tgaatagctg ccgagcagcg
    18001 cccccacgat gaccggcgac agtgtcagct gctgccaatc gaaccagttg ggcaccgggg
    18061 tccttcgcgc cagccggcgc atggccatga acaacgctat ggtcatggcc gccaccaacg
    18121 cggctcccac gaaggtatgc aggaaacgat gcaatggaaa gttgccgcgc aggatgttgt
    18181 aaatggactc gcagtccgcg atgcagttgg cggccatgaa cgcgatgaag ctcacccggc
    18241 gcggcgctgc ggcgtgaaac aacacgccgg ggccgaagtg aaatggcgta attggcacgc
    18301 ctaggaattc ctgaagctca agccggcttt ctcgggagac gcacgcaatt tcggcatcag
    18361 acgtctttgg cgtaaacgat gaaaccgaag tgtttcgcca ccttgtcgta cagagttcga
    18421 ccgggcgtgt tcgtggtgtg cgtctgccag tacacgcgtt tgcagcccgc atctttggca
    18481 atttcataga cgcgctcgat gagcgcgcgc ccgatgccgt ggccgcggta gtcaggggcc
    18541 gtaaacaggt cctgcagata acacagcagc tccatgcgcg aagtgctgcg gtgatagatg
    18601 aagtggcaca tgccgaccac cttgtgctcg cgctccgcca cgatgcagtg caccggttcg
    18661 ctggcatcga aaaaccgccg ccacagcgtg tcattcaatt cttcggacag cgcggtgggg
    18721 ccagtgcgtt cgtagaacgt gttgtattca tcccagagcg gtctccatgc atcgtggtcc
    18781 tcgggactga cagggcggac ggtgatggac gctcgtttct tcgtcatttc ttcggttctg
    18841 ccttcggcgt ggatgcggtt gagttttcag cgggcaccag ttcacccaca cacatcggga
    18901 aatcgcggac gagttcgttc actgcgctct cgtacttcgc gtagctcatc tttccagaat
    18961 tggcggccag cgcgtcgcgc ttcagccacc actgctgcac gctaccgaca acccacgcat
    19021 tcctgattcg tgacaactgt agccctttcg atgactgcgg ggtctccgca acgatgtaca
    19081 cctggtcggt gcattgcggg ccctcttcgc acggacaccc ggtaaccacc ggcgagatgt
    19141 tcagcagcac ctgcggcagg tacttctcgg ccacgagctg aatttcgcga acctcatagt
    19201 cggagatgtt gagataacgt agcggctcat cccgacgctt cgggcgaagt tcgtacgccc
    19261 gatccacgat gcgcattcgc ttcaccatct gcgatcgata ggcgtcccat tccgctttcg
    19321 aggcgggagc ttgagcactc gccgatgccg cgaggagcaa acccatcgcc agaattgaaa
    19381 cgtttcgcat gattgccatc tccccgctgc cggatgctat tggtggcacc tgcaggcgcg
    19441 caaatgcctg tctgaattga ggctttttac cgggcaaagg ttgaagggaa gccacgataa
    19501 accagcaaaa gcccgttttg cccctgttct tctcaaataa gaataattat catctggccc
    19561 tctaatcggc ccttcggggc ctccgggacc agccagtcaa tcctgatgcc cttcgggctc
    19621 aaggccgcca gccatcccct
//
LOCUS       DASQJS010000235         5198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1076857, whole genome shotgun sequence.
ACCESSION   DASQJS010000235 DASQJS010000000
VERSION     DASQJS010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076857"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VEW08_09535"
     CDS             complement(<1..978)
                     /locus_tag="VEW08_09535"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYJ41021.1"
                     /translation="MQPIECISAQSEGVARRAEMNLATNPKDDGVQLPSGVSPAISAV
                     NRLIGQVAPYDSTVLILGESGTGKEIAARAVHAASPRRQRPFVAVNCGAIPAELLESE
                     LFGHEKGSFTGAITTRKGRFEIAEGGTLFLDEIGDMSLPMQVKLLRVLQERVYERVGS
                     NVSSHCDVRIIAATHRNLEESIVRGTFRGDLFYRLNVFPIEMPTLSERIGDLQILIND
                     FVTVAASRGRPRLQLLPETLDALAGYSWPGNIRELSNLIERLAILCPERAVGVGDLPA
                     RYRPADWTAPQVTLTPTLEIPQLLASMSGDAEIEEDVELPASIWLGEQDS"
     gene            complement(1153..1719)
                     /locus_tag="VEW08_09540"
     CDS             complement(1153..1719)
                     /locus_tag="VEW08_09540"
                     /inference="COORDINATES: protein motif:HMM:NF028133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HYJ41022.1"
                     /translation="MYDGKDTIVIFDKLAYEDLLPVRWRPYPQQVDDVTAQHFAERNL
                     RVLQACDALEEHGQLDKKDDEAPHSADLRRLDFKLNLLLDLAGQLLAQSQPRLKPSTI
                     RLNAMGASFTYDDGLVEGAYGVLEVMLRDIVVQPLNLPAQVVSGAPQGQTRVRFLSLG
                     EVVADHIEKLVFRRHRRKIAGARGARRA"
     gene            complement(1732..2058)
                     /locus_tag="VEW08_09545"
     CDS             complement(1732..2058)
                     /locus_tag="VEW08_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41023.1"
                     /translation="MSVPASQARRPSHLRLVRSSLAPVLRAESDGGWRRVADAILAST
                     WDVTRFLSQKRWGRVHEELSERRELLGLMRSMKLDANGRSCFRSLDEAAVESERAIAA
                     MMVESR"
     gene            complement(2055..2468)
                     /gene="fliS"
                     /locus_tag="VEW08_09550"
     CDS             complement(2055..2468)
                     /gene="fliS"
                     /locus_tag="VEW08_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HYJ41024.1"
                     /translation="MAFPPNAAKLAAYRSTAVHSGVDAADPHRLIVMMMDGALERIAT
                     ARGMMNHGGGAEKAQLLHRAVAIIDELRNCLDFNAGGELAKNLDSLYEYMCQRIMQAN
                     ANNKPEWLDEVSRLLGEIRSAWLQLPQARGQAGAR"
     gene            complement(2621..4003)
                     /gene="fliD"
                     /locus_tag="VEW08_09555"
     CDS             complement(2621..4003)
                     /gene="fliD"
                     /locus_tag="VEW08_09555"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HYJ41025.1"
                     /translation="MAGLSSPGLGSGLDINGLVSQLVAAEKAPAQAQITRSQTSTATS
                     ISALGQLKGALGAFNAALTSLKSLETFSTRSATASDASKFTASASIAAAEGSYDVQVE
                     SLASAHQLTSDPFVEGAAQEVGTGTLTIGSGDKTFQVSIDATHNTLAQIRDAINQATG
                     NTNLVRATIVNAADGAHLVLSATATGEDAAITVAQADGDGGLASLVYNPSLTTNYTQQ
                     RPAADSVVFIAGFEHNSATNTVADAIDGVSITLLDAEPGEIFTLTIANDTAATTGRIK
                     NFVDQYNSLAKTITALRGYEPTTKKAGPMLGDAMLRGIESELRNKLTDTVSGLSGPYQ
                     SLASIGITTQKDGTLKLDAAKLDTAMKSNYDGVATLFSSATGVATRLSDALTPRLETG
                     AEMDVRSKRLNAKSVELQKSQATLEARMLKVEARYRAQFTALDSLLSKLQGESSFLTQ
                     QLSSISKIGD"
     gene            complement(4029..4385)
                     /locus_tag="VEW08_09560"
     CDS             complement(4029..4385)
                     /locus_tag="VEW08_09560"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HYJ41026.1"
                     /translation="MKVGEVLQEADVRIQRKAQADRAAAATAAANVERVPAAKPVQPV
                     EVASIESVRRQIDTFLRSTNLALQFRVDDATGEMVVSVTDAETGEVIRQVPGEEALRM
                     AERIDANIGALLDEKV"
     gene            complement(4499..>5198)
                     /locus_tag="VEW08_09565"
     CDS             complement(4499..>5198)
                     /locus_tag="VEW08_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HYJ41027.1"
                     /translation="VTSGNVAVVVADAATAAATGTALRTAIQGQYTLGNTSLMATGTN
                     AILLVNDKLGANGTVAEYLDESGITHGGDATAVTVSALSGGGTDGDYDAGITTRGTIT
                     LSSAANFTLGGTDLAAGGLDTAAASLTRLDSVNISTVAGANAAIAVLDGALSQVTSMR
                     ADLGAVQNRFSSTVANLTTTAENLNAARSRILDADFAAETAALTRAQILQQAGTAILA
                     QANSVPQNVLSLLR"
BASE COUNT          842 a         1616 c         1729 g         1011 t
ORIGIN      
        1 ggagtcctgc tcgccgagcc agatggacgc cggcaactcc acatcctctt cgatctcggc
       61 gtcgccggac atcgaggcca gcaactgcgg gatttccagc gtcggcgtga gcgtgacctg
      121 cggagcggtc cagtccgcgg gacgataccg cgccggcagg tcgccgacgc ccaccgcacg
      181 ctcgggacac aggatggcca gccgttcgat gaggttggaa agttcgcgga tgttgcccgg
      241 ccacgaatac ccggcaagtg cgtcgagcgt ctcgggcagc agctgcaggc gcgggcgtcc
      301 gcgcgaggcg gccaccgtca cgaaatcatt gatgagaatc tgcaggtcgc cgatgcgttc
      361 gctgagcgtc ggcatttcga tggggaatac gttgaggcgg tagaacaggt cgccgcggaa
      421 ggtgccgcgc acgatggact cttcgaggtt gcgatgcgtc gcggcgatga tgcgcacatc
      481 gcaatggctc gatacgttgc tgccgacgcg ttcataaacc ctttcctgca ggacgcgcag
      541 caacttgacc tgcatcggca ggctcatgtc gccgatctcg tcgaggaaca gcgtgccgcc
      601 ctcggcgatt tcaaaacggc ccttgcgggt agtgatggcg ccggtgaagg aacccttctc
      661 gtggccgaac agttcgctct cgaggagctc ggcgggaatg gcgccgcaat tcacggcgac
      721 gaaggggcgt tgacggcgcg ggcttgctgc atggacggcg cgcgcagcaa tctctttgcc
      781 ggtaccagat tcccctagta tgagaaccgt ggaatcgtac ggggccacct ggcctatcag
      841 ccggttgacg gccgagattg ccggggaaac tccggagggc aactgaacac cgtcatcctt
      901 cgggttcgta gccaggttca tctctgcccg gcgcgcgact ccctcgctct gcgcactgat
      961 gcattcgatc ggctgcacgg taactcctta cgtctctcgc gagtatcttc gcgcgcttga
     1021 ctcgcgtgaa agcgtgatgc gccgtgcatc tttgcagaca cgaaggagaa atgacctaac
     1081 aactttgcgc gcgctgccga tgacaagcgc cgcgcgaggg attcccttac tgcttagtcc
     1141 ttagtgaaaa cactacgcgc gtcgagcgcc gcgcgcgccg gcaatttttc gccggtggcg
     1201 gcgaaacacc agcttctcga tgtgatcggc gactacttca ccgagcgaga gaaatcgcac
     1261 gcgtgtctga ccctgcggcg cacccgacac gacttgcgcg ggaagattga gtggctgcac
     1321 gacgatgtcg cgcaacatca cctcgagtac gccgtacgcg ccttcgacca agccatcgtc
     1381 gtaagtgaaa ctcgcaccca ttgcgttcag gcggatggtg ctcggcttca gacgcggctg
     1441 gctttgcgcc agcagctgtc cggcgaggtc gagcagcagg ttcagcttga aatcgagccg
     1501 gcgcagatcg gccgaatggg gagcttcgtc gtctttcttg tcgagctgcc cgtgttcttc
     1561 gagcgcatcg caggcctgaa ggacgcgcag gttgcgctcg gcgaagtgct gggcggtgac
     1621 gtcgtcaact tgctgcggat aggggcgcca gcgaaccggc agcaggtcct cgtaggccag
     1681 cttgtcgaaa atgacgatgg tgtctttgcc gtcgtacatg cggcagggag tctagcggct
     1741 ttcgaccatc atcgcggcaa ttgcgcgctc cgactcgacc gccgcttcgt cgagcgaccg
     1801 gaagcaggag cggccattgg cgtcgagttt catggagcgc atcaggccga gcagctcgcg
     1861 ccgctcgctc agttcctcgt gcaccctgcc ccagcgcttc tggctcagga aacgtgtgac
     1921 atcccaggtg ctggcgagaa tggcgtcggc gacgcgacgc cagccgccgt ccgattcggc
     1981 gcgcagcacg ggcgcaagcg agcttcgcac cagccgcagg tgcgaaggac ggcgcgcttg
     2041 tgaggcggga acgctcatcg cgcaccggcc tgcccgcgcg cctgcgggag ttgcagccag
     2101 gcggaacgga tctcgccgag cagccggctc acttcgtcga gccactcggg cttgttgttg
     2161 gcattcgcct gcatgattcg ctggcacatg tattcgtaca gcgaatcgag attcttcgcg
     2221 agttcgccgc cggcgttgaa gtcgaggcag ttgcgcagtt cgtcgatgat ggccacggcg
     2281 cgatgcagga gctgcgcctt ctcggcgccg ccgccgtgat tcatcatgcc gcgcgcggtc
     2341 gcgatgcgct cgagcgcgcc gtccatcatc atgacgatga ggcgatgcgg atccgcggca
     2401 tccactccgc tgtgcacggc ggtgctgcga taagcggcga gttttgcggc gttgggcggg
     2461 aaggccatcg tcagttgctc catgtttgtc aaggagctta acggcctagc aacgccggac
     2521 ttgagtgtgg tgccgggccg gattggagtc tgacaatgcg acggagcggg ctcggtggcg
     2581 cgatgggaaa gttttcccgg tttcccggcc cggcaccttc ctagtcgccg atcttcgaga
     2641 tactgctgag ctgctgcgtg aggaacgagg actcgccctg cagtttcgac agcaggctgt
     2701 cgagcgcggt gaactgcgcg cggtaacgcg cctccacctt gagcatgcgg gcctcgagcg
     2761 tcgcctggct cttctgcagt tcgacgctct tcgcattcag gcgtttcgag cgcacgtcca
     2821 tctcggcgcc ggtctccaga cgcggcgtca gcgcatcgga cagtcgcgtg gccacgccgg
     2881 tggcggagct gaacagcgtg gcgacgccgt cgtagttgga tttcatcgcg gtatcgagct
     2941 tggcggcgtc gagcttgagc gtgccgtcct tctgcgtggt gatccctatg ctggccagcg
     3001 actggtaggg gccggagagg ccgctcaccg tgtcggtgag cttgttacgc agctcggatt
     3061 cgatgccacg cagcatggcg tcgcccagca tcgggcccgc cttcttggtg gtcggctcgt
     3121 agccgcgcag cgcggtgatg gtcttggcca gtgagttgta ctggtcgacg aaattcttga
     3181 tgcgcccggt ggtcgccgcg gtgtcgttgg caatcgtgag cgtgaatatc tcgcctggct
     3241 cggcatcgag cagcgtgatg ctcacgccgt cgatggcgtc ggcgacggtg ttggtggcgc
     3301 tgttgtgctc gaacccggcg atgaatacca ccgaatcggc cgccgggcgc tgctgggtgt
     3361 agttggtagt gagcgacggg ttgtacacga ggctcgcgag cccgccgtcg ccgtcggctt
     3421 gcgccacggt gatcgcggcg tcttcacccg tggcggtggc tgacaacacc aggtgcgcgc
     3481 cgtccgcggc gttgacgatg gtagcgcgca ccaggttcgt gttgccggtg gcctggttga
     3541 tggcgtcacg gatctgcgcc agcgtgttgt gggtggcgtc gatgctcacc tggaaagtct
     3601 tgtcgccgga accgatggtg agcgtgcccg tgcccacttc ctgcgctgcg ccttccacga
     3661 acggatccga tgtgagctga tgcgccgacg ccagggattc gacctggaca tcgtagctgc
     3721 cttccgccgc ggcaatggag gcggaggccg tgaatttgct ggcgtctgat gcagtcgccg
     3781 agcgcgtgga gaaagtctcc agcgacttga gcgacgtcag tgccgcgttg aatgcaccga
     3841 gcgcgccctt gagctgccct agtgcggaaa tactggtggc cgtggaggtc tgcgatcgcg
     3901 tgatctgcgc ctgcgcgggt gctttttcgg cagccacgag ctgtgaaacc agaccgttga
     3961 tatccagtcc ggaccctaaa ccgggtgagc tcagtcctgc catgtcgttc tgttcctgtt
     4021 caaatcaatc aaactttctc atcgagcaag gcgccgatgt tcgcgtcaat gcgttccgcc
     4081 atgcgcaacg cctcttcgcc cggcacctgg cgaatcactt cgccggtctc cgcatcggtg
     4141 acgctcacga ccatctcgcc ggtggcgtcg tcgacgcgaa actgcagcgc gagattcgtg
     4201 ctgcgtaaaa acgtatcgat ctgccgtcgc accgattcga tgctggcgac ctccacgggc
     4261 tgtaccggct ttgccgccgg taccctctcg acattcgcag cagccgttgc tgctgccgcg
     4321 cgatcggctt gcgcctttcg ctgaattctt acgtcggctt cctgtaaaac ttcaccgact
     4381 ttcatggctg tacctcagca ggtctccagg gtctgccgct tcaggcagga gccgcgggtc
     4441 ttgcgacccg cggcccctcc attacggcta tctgaagctc cgctcagatg tcaccccatt
     4501 accggagtaa cgagagaacg ttctgcggga ccgagttggc ctgagcgaga atcgcagtac
     4561 cggcctgctg cagaatctgc gcgcgggtca gggcagcggt ttccgcggcg aagtcggcat
     4621 ccaggatgcg gctgcgcgcg gcgttcaggt tctcggcagt cgtcgtcagg ttggcgacgg
     4681 tcgacgagaa gcggttctgc actgcgccca ggtcggcgcg catgctggtc acctgcgaca
     4741 gggcgccgtc gagcacggcg atcgccgcgt tcgcaccagc aaccgtcgag atgttcaccg
     4801 agtccagacg cgtcagcgag gccgctgccg tgtcgagacc accggcggcg agatccgtgc
     4861 cgcccagggt gaagttcgca gccgagctca gcgtgatcgt gccacgcgtg gtgatacccg
     4921 cgtcgtagtc gccgtccgtg ccgccgcccg agagcgcgct gaccgtaacg gccgtagcgt
     4981 caccgccgtg ggtgatgccc gactcgtcga ggtattcagc aaccgtaccg ttcgcaccga
     5041 gcttgtcgtt gaccagcagg atcgcgttgg tgcccgtggc catcagcgag gtgttgccga
     5101 gcgtgtactg accctggatc gccgtgcgca gcgcggtgcc ggtcgccgcc gccgtggcgg
     5161 cgtccgccac gacaactgcg acattgcctg aagtgaca
//
LOCUS       DASQJS010000236         9024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_136467, whole genome shotgun sequence.
ACCESSION   DASQJS010000236 DASQJS010000000
VERSION     DASQJS010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136467"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3004)
                     /locus_tag="VEW08_09570"
     CDS             complement(<1..3004)
                     /locus_tag="VEW08_09570"
                     /inference="COORDINATES: protein motif:HMM:NF025295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HYJ41028.1"
                     /translation="MRSGLALILCVFSLLAGCGGGDGGTTPPPASNNPPPPPPPPPPP
                     PVTGAAGGTVTEVSGASVIVPAGALTADTTIRIARDSTGAPPISADLTPAGSMYVITP
                     HGGEFAAGVEVRLPKPNVTLTEFQEFKIAKAEPGEPWIVLEDTVLNQDSLNVKVDSFS
                     YFTAVVVTYLLPIAQSVPMNMDTSIACTGGSCSGLIGPTTATFTVTSNNGQTPGNCTN
                     PIYGIGAAAGNIPWPSTGSNIGVAPLAATGGSVTRTLNPIPRLSFKFGVGRKCGVYGT
                     YSDFGYDNYKHVYWAASFGPTLSIVQMPAQVDVVEGFAANVDIVMAGGAAEPADRNNP
                     ANSTYAVPTRTNRAIIDWERSDDNGASWRVVSRSYQNEANSLPGGVGFAWRYWGIRHG
                     FVATATDQGALLRIHACYTPPAPAEAPPCIDSATSRINVLQQSARPTIVDAPHSMLVR
                     RGQTASLSATASGLPAPTLQWQSRPANSTGAWSDVATGAGATTSNYTTPALALADNGT
                     QYRVVATNAVGSVESTTVTVSVSELDVAPTIASQPSSLSVASGSDAVFAVDAHGTEAL
                     SYQWYRNGSALAGANSPVLRLTGVTLVNAGNYTVTVSNSAGDADSNAATLNVYPGTPA
                     AIAPTIVTQPAAVTVNAGNTATFAVGVDGSGPFTFQWRRDGVNVSGATSAVLTFNSVA
                     LPNAGTYSVVVTNSAGAIVSNNTTLDVGPAAGAVAPTITSQPATVIVPTGGSAVLAVA
                     ATGSGPLTYQWTRDGVPIGGATQAVLILPTVDVSNAGDYRVTVGNSLGDVVSQTATVI
                     LLGAPVIYTSPSNVSVFENSTTTFRVDSAGSNRHFQWLRNGSPIASSDSDAYTTPSLT
                     VADSGAVYSVIVYNGAGLVVSAPAVLTVRVPVPPTILQQPADASIQAGATASLCMAFG
                     GTPPFAVQMNRWLNNQWTPMGPAQSFTDNAAHCIATPALQSADNGAQFIFFASSDEGG
                     YAEAMTRTVTVTVAAPSGITTTTLASRAT"
     gene            complement(3098..4414)
                     /locus_tag="VEW08_09575"
     CDS             complement(3098..4414)
                     /locus_tag="VEW08_09575"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HYJ41029.1"
                     /translation="MPRAARALAVWANGVRVGLWRLPSRGPMEFQYDDSWLHAVEARP
                     LSLSLPLPLDDHAVKGPAVEAYFDNLLPDSEPIRRRLQERFHTASRNAFDLLAAIGRD
                     CVGAVQLLPPDAPAPDIRKIDATPLSEAAITRELSAVTSTSSIAGDDAEFRISIAGAQ
                     EKTALLRHHGRWCRPMGSTPTTHIFKLPLGLVGNRQADMRTSVENEWLCARIVAAYGI
                     PVAPCDIDEFGANKVLIVERFDRVLHSSGKYWLRLMQEDFCQATATPWHRKYQADGGP
                     GLVDITRILRRSTESQRDFATVFRAQVLFWMLAATDGHAKNFSIRILQGGAFQLTPLY
                     DVLSAWPIIGKKANEIPPEKVKMAMAMPGQRPHYLHKTIQRRHFETLAKRMGLESHAE
                     RMIAEIVTKTPSVIEQVQQGLPKAFPQALLDRVLVGLERTSRVLAN"
     gene            complement(4418..4660)
                     /locus_tag="VEW08_09580"
     CDS             complement(4418..4660)
                     /locus_tag="VEW08_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ41030.1"
                     /translation="MHPINDPRQIGATLVGRRKHLKLSQTDVAARLGLSQNRLSELES
                     SPQSLTVEQLLALLNVLGLEMSLRERDAGGKPKVEW"
     gene            4921..6858
                     /locus_tag="VEW08_09585"
     CDS             4921..6858
                     /locus_tag="VEW08_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HYJ41031.1"
                     /translation="MASDAGKASRRVLGRDARLQSAERRCADYRRAFQLILDEPFDDL
                     DKTIDRMLQILATTLDVGRVSFWTVDTNYTSIRCEHVFTVDRSRPLGATLLKRADCPT
                     YFETLCQELVIAADDARNDPRTCEFSASYLEPLGVVSLLDVPVRAFGRYVGVLCHEQF
                     DEKRSWTREDEHFASGVATQIALAYERDRAKRAQEKLLERSLRDEESQLANRVHLEQA
                     LTAYLQNPARAGAVLIMSADQHSFVASCLGARRMQLLLREFAGRLAIAGPDGTLVART
                     GTNQFAMLLRGVEKWELPRILTQVNASLTTPLVSEGQRLFFTLSGGYAYIDSEIEQDA
                     ETLLNEAQMACHEARAAGGDRATPFTAEMRHAMRGRISLEQDLRRALDAAEFDLHFQP
                     IVSLNEGGDMSLEALLRWRHPRLGVLSPESFIQVAIDSGIMLDLGRRVLANACTSLAK
                     LQSQKGMEQLGMTVNMSAPEVLLPGTAEAIQMELLRNGLPARALTIEITETAFMVDLD
                     RAASAIAEIREIGVSISLDDFGTAYSSLSWLRKLAIDKVKIDRSFVAGIENVPEDLAI
                     VRSVVDLTRAFKRDVVAEGVENLNQLRILRALGVDHAQGYLFSRPEPLEKFDAARLNS
                     LRSIDRGLDVADARTAHSQSG"
     gene            complement(6800..7174)
                     /locus_tag="VEW08_09590"
     CDS             complement(6800..7174)
                     /locus_tag="VEW08_09590"
                     /inference="COORDINATES: protein motif:HMM:NF022317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HYJ41032.1"
                     /translation="MKFAIAADAVMAFHFGFIAFALLGSFLLLRWPKVIWLHLPALAW
                     GAYIELSGNICPLTPLENHFRALAGESTYGEGFIVHYLEPIIYPAGLTRGGQHLALAI
                     LLATNLTGYALFWRRRRRGPDR"
     gene            7396..8358
                     /locus_tag="VEW08_09595"
     CDS             7396..8358
                     /locus_tag="VEW08_09595"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HYJ41033.1"
                     /translation="MRPAHAVSIDPGTWAVETELGYQNTWALSPEVEKYLVDLEPSGR
                     RALGAAEMQAIEDLPGENYLLDIETATFDLTLHYKIAADWSVYGIISAISYQGGFLDS
                     TIENFHDALGFSSFGRPAAARNQAVLIYDLKGVHVAAMEKPTDGGLTDPTIGFRYSGV
                     RFSPDWQLTLEGAVKIPVAGRRLLLSTGRSDYGLQASLQRLGIRHAFYGNLAAVYYSG
                     TREPAPQNAQIVPTVIVGYEFKWTAKTNLNIQAYVSNSVYSHEQTDLEDLLGMKYQYS
                     LGLRHRMENWLFSFGVTENVQNVNNTPDIGFQFGVAWIPRQRLK"
     gene            8495..>9024
                     /locus_tag="VEW08_09600"
     CDS             8495..>9024
                     /locus_tag="VEW08_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41034.1"
                     /translation="MRDLAPDIVRQRLLIEGLFDIEVDGATVERYLIDLAAHLNLRTY
                     GKPIVHAPGGAGKDDNEGFDAFIPLIDSGISLYVWSRKRFFSDRWRIDLALEVTMSGD
                     CGNQGFHGVVNLCIPQQACIGTLALGRRGNAGSVQEFTARTQGQDGLGVAELILREFQ
                     GLSGLGQCGDTGIAIG"
BASE COUNT         1665 a         2678 c         2958 g         1723 t
ORIGIN      
        1 tggtcgcgcg gctcgcgagc gtcgtggtgg tgattccgct cggcgccgcc accgtcacgg
       61 tcaccgtgcg cgtcatggct tcagcgtagc cgccctcatc gctgcttgcg aagaagatga
      121 actgcgcgcc gttgtccgcg gactgcagag ccggcgtcgc gatgcaatgc gcggcgttgt
      181 cggtgaagga ctgggcggga cccatcggcg tccactggtt gttcagccag cggttcatct
      241 gtacggcgaa cggcggcgtt cccccgaagg ccatgcaaag actcgccgtt gcgcctgcct
      301 ggatgcttgc atccgccggt tgctgaagga tcgtcggcgg cacgggcacc cgtaccgtca
      361 atacggctgg cgcgctcacg acgagaccgg cgccgttgta cacgatcacg ctatagacgg
      421 cgccgctgtc ggccacggtc agcgacggcg tggtgtacgc atcggaatcg ctggaagcaa
      481 tcggtgagcc gttgcgcagc cactggaagt ggcggttcga gccggccgaa tccacgcgga
      541 acgtcgtggt cgagttctcg aatacgctga cattcgaagg actggtgtag atcaccggtg
      601 cgccgagcag tattacagtc gcggtctggc tcacgacgtc gcccaggctg ttgccgacgg
      661 tcaccctgta gtcgccggcg ttcgatacgt ccaccgtggg aaggatcagc acggcttgag
      721 tcgcgccgcc gatgggcacg ccatcccgcg tccattgata cgtgagcggg ccgctgcccg
      781 tggccgccac agcgagcacg gctgagcctc ccgtcggaac aataaccgtc gcgggttgcg
      841 aggtgattgt cggcgccaca gcgccggcgg cgggtcccac gtcgagcgtg gtgttgttgc
      901 tcacgatcgc cccggcactg ttcgtgacca cgacggagta cgttcccgcg ttcggcaacg
      961 ccacggagtt gaacgtaagc actgcgctgg tggcgccgga cacgttcacg ccatcacggc
     1021 gccattgaaa ggtgaagggg ccgctgccat cgacaccgac ggcaaacgtt gcggtgttgc
     1081 cggcgttcac ggtgacggcg gcgggctgcg tcacgatggt cggcgcgatc gcggcgggcg
     1141 tgccgggata cacgttgagg gtggcggcat tgctgtccgc atcaccggcg gaattgctca
     1201 ccgtcaccgt gtagttgccg gcgttgacga gggtcacgcc agtaaggcgc agcaccgggc
     1261 tgttggcgcc ggcgagtgca ctgccattgc ggtaccactg ataactcagc gcttcggtgc
     1321 cgtgtgcgtc caccgcgaac acggcgtcgc tgccggaggc cacgctcaag ctcgagggtt
     1381 ggctggcgat ggtcggtgcg acgtcgagtt cactgaccga aaccgtgacg gtcgtgcttt
     1441 ccacgctgcc cacggcattg gtggcgacca cgcggtactg cgtgccgtta tcagccagcg
     1501 ccagtgcggg ggtggtgtag ttagaggtgg tcgcgccggc gccggtggcg acatcactcc
     1561 atgcaccggt ggagttggca gggcgtgact gccactgcag cgtgggtgcg ggcagtccgg
     1621 aagcggtcgc cgacaggctg gcagtctgac ccctgcggac aagcatcgaa tggggcgcat
     1681 cgacaatcgt cggccgcgcg ctctgctgca gtacgttgat ccgtgatgtg gcgctgtcta
     1741 tgcagggagg cgcctcggca ggcgccggcg gtgtgtagca ggcatggatt cgcagcagcg
     1801 caccctgatc ggtggctgtg gccacgaaac cgtggcggat gccccagtaa cgccacgcaa
     1861 atccaacgcc accagggaga ctattggcct cgttctgata tgaacgcgat acgactctcc
     1921 acgaggcacc gttatcgtca ctgcgctccc aatcgatgat ggcgcggttg gtgcgcgtcg
     1981 gaacggcata ggtgctattc gcgggattgt tgcgatcggc gggctccgct gcgccgccgg
     2041 ccatcacgat gtcgacattc gctgcgaagc cttcgacgac atccacttgt gcgggcatct
     2101 gcacgatgga gagggttggg ccaaatgaag cagcccagta gacgtgcttg taattgtcat
     2161 atccgaagtc ggaataggtg ccgtacaccc cgcatttgcg tcccacgccg aacttgaaac
     2221 tgagtctcgg aattggattc agtgtgcgtg tgacggagcc gccggtggct gccaggggtg
     2281 caacgccaat attggagcca gtggatggcc aggggatatt gcccgcagcg gctccgatcc
     2341 cgtaaatggg gttggtgcag ttcccgggcg tctgaccatt gttggaggtg acagtaaagg
     2401 tggccgtcgt cggtccaatc agtccgctac aggaaccgcc tgtgcacgcg atcgaagtat
     2461 ccatgttcat gggtacagat tgcgcgatgg gaaggagata ggtgacaacg acggctgtga
     2521 agtacgaaaa gctgtccact ttcacattga gtgaatcctg attcagcact gtgtcttcga
     2581 gcacgatcca gggctcgccg ggctccgcct tcgcgatctt gaattcctgg aattccgtca
     2641 gcgtcacgtt cggtttcgga agtcgcactt cgacaccggc ggcgaattcg ccgccatgcg
     2701 gcgtgatcac atacatgctg ccggcgggag tcagatccgc cgaaatcggc ggcgcaccgg
     2761 tcgaatcgcg cgcaatgcgg atcgtggtgt cggctgtgag tgcaccggca ggcacgatga
     2821 cggacgcgcc gctcacttcc gtcacggttc caccagccgc gccggtgaca ggcggcggcg
     2881 ggggtggggg tggaggcggg ggtgggttgt tggacgcagg cgggggcgta gttccgccat
     2941 ccccgccgcc gcaaccggct aacaagctga atacgcacag aatcaacgct agacccgaac
     3001 gcatgatgct cacctggttg ttgcccgtaa gaccaacgcg gcaagacctg ggaaaccggc
     3061 tcaggagtta tcgcaagaat cgggtcttac ataactatca gttcgcgagc acgcgggagg
     3121 ttcgttcgag tccgacgagc acccggtcga gcagggcttg cggaaatgcc ttcggcaagc
     3181 cctgctgcac ctgttcgatc accgacggcg tcttcgtcac gatctccgcg atcatgcgct
     3241 cggcatggga ttccagcccc atgcgtttgg cgagcgtttc gaagtgccgt cgctggatgg
     3301 tcttgtgcag gtagtgaggc ctctgtcccg gcatggccat ggccatcttc accttttctg
     3361 gcgggatctc gtttgctttt ttgccgatga tgggccaggc ggaaagcacg tcgtacagcg
     3421 gcgtgagctg aaaggcaccg ccttgcagaa tgcgaatgct gaagttcttg gcgtgtccat
     3481 ccgtggcggc cagcatccag aacaggacct gcgcacggaa gacggtcgcg aagtcgcgct
     3541 gagattcggt cgagcgccgc aggatgcgcg tgatgtcgac gagcccggga ccgccatcgg
     3601 cctggtattt gcggtgccac ggtgtcgcgg tggcctggca gaagtcctcc tgcatgagcc
     3661 gaagccagta tttgccgctg gagtgaagca cgcggtcgaa gcgttcgacg atcagcactt
     3721 tgtttgcgcc gaactcgtcg atgtcgcagg gcgccacggg gatgccgtag gcggcaacga
     3781 tgcgcgcgca cagccattcg ttttctacag aggtgcgcat gtcggcctgc cggttgccga
     3841 caagtcccag cggaagtttg aagatgtgcg tggtgggtgt ggaacccatg ggcctgcacc
     3901 atctgccgtg atgccgcaat agcgctgtct tctcctgcgc acctgcgatg gagatgcgga
     3961 actctgcatc gtcgccggcg atggaggagg tggaggtgac ggcgctgagt tcgcgggtga
     4021 tcgccgcttc gctgagtggc gtggcgtcta tcttccggat atccggagcg ggtgcatccg
     4081 gcggcagcag ctgtacggcg ccgacgcagt cgcggccgat ggcggccagc aggtcgaatg
     4141 cgttacggga tgcggtatga aagcgctcct ggaggcgcct gcgaatgggc tcgctgtcag
     4201 gcagcaggtt gtcgaaatat gcttcgacgg ccggaccctt gaccgcatgg tcgtcgagtg
     4261 gcagtggcaa ggagaggctc agtggccggg cttccaccgc gtgcagccag ctgtcgtcgt
     4321 actgaaattc catggggccg cgcgagggca ggcgccatag tccgacgcgg actccgttcg
     4381 cccagacggc cagggctcgg gcggcgcgcg gcacgagtta ccactcgacc ttgggtttgc
     4441 cgccggcgtc acgctcgcgc aggctcatct cgaggcctaa cacgttgagc aaggcgagca
     4501 gttgctcgac agtcagcgac tgcggactcg actcgagctc ggagagccgg ttctgactca
     4561 ggcccaggcg ggcggcgacg tcagtctgcg agagcttgag atgtttgcgg cgcccgacga
     4621 gggtggcgcc gatctgccgt gggtcgttga tggggtgcat ggcggaatat cggtaatggc
     4681 gataactgca atatatcggt tataccgata ctgtcaagat atcggctata ccgataatcc
     4741 acgccggaat gcgacactta tatatgcgtc ctggcaaaac gctggaacat ggaagccgcg
     4801 caggccatcc ctttacgcct tccttttgtg atccggtaca agaccctgta acccgccggt
     4861 ggccaggtag gcagggcggc ggtagagttg tcgaaaatca gccacgcccc ggggagaagc
     4921 atggctagtg atgccggaaa ggccagtcgg cgggtattag gccgcgacgc aaggttgcag
     4981 tcggcggagc gacgctgtgc tgactatcga cgtgctttcc agctcatcct cgatgagcct
     5041 ttcgacgatc tcgacaagac catcgaccgg atgctccaga tcctggccac gaccctggat
     5101 gtcgggcgtg tgagcttctg gaccgtcgac accaactaca cctccatccg ctgcgagcac
     5161 gtattcaccg tcgaccggtc ccggccgctc ggggcaacgc tgctcaagcg cgcggattgc
     5221 cctacctact ttgaaacgct gtgccaggag ctggtcattg cggccgacga tgcgcgtaac
     5281 gatccgcgca cctgcgagtt ttcggcgtcc tacctggagc cgctgggcgt cgtttcgctg
     5341 ctggatgtgc ccgtacgcgc gttcgggcga tacgtgggcg tcctttgcca cgagcaattc
     5401 gatgagaagc gctcctggac gcgcgaagac gagcacttcg cgtcgggcgt ggcgacgcaa
     5461 atcgcactgg catacgagcg cgaccgcgcc aagcgcgcgc aggaaaagct gctcgagcgc
     5521 tcgttgcgcg acgaagagtc gcagctcgcg aatcgtgttc atctcgaaca ggcgctcacg
     5581 gcatacctgc agaaccccgc gcgggccggc gccgtgctca tcatgtccgc cgatcagcac
     5641 tcattcgtcg ccagctgcct gggggcccgg cgtatgcaac tactgctgcg cgagtttgcc
     5701 ggcagactcg caattgccgg accggatggc acactggtgg cgcgcaccgg aaccaaccag
     5761 tttgcgatgc tgttgcgcgg agtcgagaag tgggaactcc cacgcatcct cacgcaggtg
     5821 aacgcgagcc tgacgacgcc gctcgtgagc gaaggccagc ggttgttctt caccttgagc
     5881 ggtggctacg cgtacatcga ttcggaaatc gaacaggacg ccgaaacact gttgaacgag
     5941 gcgcaaatgg cctgccacga ggcgcgggcg gccggcggcg accgtgcgac gccattcacg
     6001 gccgagatgc gtcatgccat gcgtggccgc atctcgctcg agcaggatct gcgccgcgcc
     6061 ctggatgcgg cggaattcga tctgcatttt cagcccatcg tgtcgctcaa cgaaggcggc
     6121 gacatgtccc tggaggcgct gctgcgctgg cgtcatccgc gccttggcgt gctttcgccg
     6181 gagtccttca tccaggtggc aatcgattct ggcatcatgc tcgacctcgg tcggcgcgtg
     6241 ttggccaacg cgtgcacatc attggcgaag ctccagtcgc agaagggaat ggagcagctc
     6301 ggcatgaccg tgaacatgtc ggcacctgag gtgttgttac ccggaaccgc cgaagccatt
     6361 cagatggagt tgctgcgcaa cggattgccg gcgcgcgctc tcaccattga aatcaccgag
     6421 accgcgttca tggtcgacct ggatcgagcc gcctcggcca ttgccgagat acgcgagatc
     6481 ggtgtgagta tcagtctcga tgacttcggc accgcctatt catcgctgtc gtggttgcgc
     6541 aagctggcca tcgacaaagt gaagatcgac cggtcgtttg tggcggggat cgagaatgtt
     6601 cccgaggacc tggccatcgt gcgctcggtg gtggatctca cgcgtgcgtt caagcgcgac
     6661 gtggttgccg agggcgtgga gaatctgaat cagttgcgta tcctgcgcgc gctcggagtg
     6721 gatcacgcgc agggatatct gttctcccgc cccgagccgc tcgaaaaatt cgatgcggca
     6781 cgactcaact cgctcaggtc tatcgatcgg ggcctcgacg tcgccgacgc cagaacagcg
     6841 catagccagt caggttagtc gcgagcagga tcgcgagcgc gaggtgctgc ccgcctcgag
     6901 tgagccccgc cggatagatg atcggctcga ggtaatggac gatgaatcct tcgccatagg
     6961 tgctctcgcc ggcgagcgcg cgaaagtgat tctcgagcgg cgtgagcggg cagatgttgc
     7021 cggacagctc gatgtacgca ccccaggcca gcgcgggcaa atgcagccag atcacttttg
     7081 gccagcgcag caacaggaac gatcccaaca gcgcgaaagc gatgaacccg aagtgaaacg
     7141 ccatgacggc gtcggcggcg atggcgaatt tcatattcag aatatagtcc gaaggtatcg
     7201 gtcaccgcac accatcgtct aatcgaaacg cgccgcccct cgtgcttcga tatcaccatg
     7261 ggcgctaaaa gagtctttgg tctcgcagtg ttgcttgccg tcgcaaagtc gaatccggcg
     7321 aacgcagagg cgggcgcatt ttacggaccg ctacgttcgc gggatttgtc gccgtttgga
     7381 tttctacggc tcgacatgcg gccggcgcat gctgtatcca tcgaccctgg gacttgggcg
     7441 gtagagaccg agctcggcta ccagaacacc tgggcgctga gccccgaggt cgagaagtat
     7501 ctcgtcgatc tggagcccag cggccgccgc gccctcgggg ccgctgaaat gcaggcgatt
     7561 gaggatctgc caggcgaaaa ctatctactc gacatcgaga cggccacgtt cgatctcacg
     7621 ctgcactaca agattgcagc ggattggtcg gtgtatggaa tcatcagtgc gatctcgtac
     7681 cagggcggct ttctcgatag cacaatcgag aacttccacg atgctctggg cttcagttcc
     7741 ttcgggcgcc cggctgctgc gcgcaatcag gcggtcctca tctacgacct gaagggagtg
     7801 cacgttgccg ccatggagaa gccaacggat ggcggtctca ccgatcccac gattgggttc
     7861 cgatattccg gagtacgctt ttctcccgac tggcaactga cgctagaagg tgcggtcaaa
     7921 ataccggtgg ctggcaggag gctactgcta tctacaggcc gatcggacta tggcctgcag
     7981 gcatcgctcc aacggcttgg aattcgccat gcgttctacg gaaacctggc agcggtgtac
     8041 tactcaggaa cccgggaacc cgcgccgcag aatgcgcaaa tcgtacccac cgtgatcgtc
     8101 ggttacgaat tcaagtggac cgcgaagacc aacctgaaca tccaggcata cgtcagcaac
     8161 agcgtctatt cacacgagca gactgatctc gaggatctgc tcggcatgaa ataccagtac
     8221 agcctcgggc tgcgtcaccg gatggaaaac tggctgttct cgttcggtgt tactgaaaac
     8281 gtgcagaacg tgaacaacac gccggacatc ggatttcagt ttggcgtcgc gtggatacca
     8341 cgtcagcgct tgaagtgaac ctcgtcacct aggagtcagt catgcataac aaatcgaagc
     8401 acctggttgc gctgggcctt tgcgccgcga tggcggtcac cgagctgcgg ccactcatgc
     8461 catcatcgcc ttgtccgggg gatggagtgg aaacatgcgc gatctggcgc cggacatcgt
     8521 ccggcagcgg ctgctgatcg aggggttgtt cgacatcgag gtcgatggcg ccacggtcga
     8581 gcgctatctc attgatctgg ccgcgcatct gaacctgcgc acgtatggca agcccatcgt
     8641 tcacgcgccc ggcggcgccg gcaaggacga caacgagggg ttcgacgcgt tcattccgct
     8701 catcgactcg ggcatttcac tgtacgtctg gagcaggaaa cgcttctttt cagaccggtg
     8761 gcggatcgac ctggcgctcg aagtgacgat gtctggcgat tgcggcaatc aaggctttca
     8821 cggcgtcgtc aacctttgta taccgcaaca ggcctgcatc ggaacccttg cccttgggcg
     8881 ccgagggaat gccggcagcg tccaggagtt tactgcccgc acccagggcc aggatggtct
     8941 tggcgtggcg gaactgatcc ttcgcgaatt ccagggcttg tccggccttg gtcaatgcgg
     9001 tgacaccggc atcgccatcg ggca
//
LOCUS       DASQJS010000237        16157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2963895, whole genome shotgun sequence.
ACCESSION   DASQJS010000237 DASQJS010000000
VERSION     DASQJS010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16157
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2963895"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VEW08_09605"
     CDS             <1..882
                     /locus_tag="VEW08_09605"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HYJ41035.1"
                     /translation="AGPDAGSVRQLEDQYSRMRSSYDESHPDMVQLRRQIDSLKFGTS
                     AGTGTSLRSQLNSKRATLAEARQRYGAEHPDIKRLQRDISTLETRIKNGERGDVEMSD
                     GTPVGMQLRTQINAIDSQIGSLMARSAELRAKVSSLERNVTAAPQVEREYANATRDLT
                     IAREKYEQLLNRQMDAEVSEAAIVGGRADEFRLIQAPMLPGAPAKPERLAILLIGLVA
                     AVVLGLTSTIAAEALDPKVRGARDIRELLSVSPLVAIPDIRNSRSRRRNTWRFVTASA
                     SGVIGVWIVFTAIVNYL"
     gene            903..1733
                     /locus_tag="VEW08_09610"
     CDS             903..1733
                     /locus_tag="VEW08_09610"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HYJ41036.1"
                     /translation="MSTTMEDAIRRNFADRSGTERPNTIETANLRQRVRRVTAAPAEL
                     QQARRFRQASVDARAMERQCILPAVSDVAALRAFKILRTRVLRRLDMHKWNSIAVSGM
                     SVCEGKTMTAINLALALSQDVNTWVFLVDLDLQRPQIANYLGMSFEKGLSDYLLGKAE
                     LEDVVYNLQSERLGVIPNARAFQHSSEFLNSPRMGEFTAALEREMPRRIVIFDMPPLL
                     ASDDVLAFAPQFDSLLLVVSEGKSGRAMLRGAKEILSEMNLLGVALNRSKERNESAYY
                     "
     gene            1759..3144
                     /locus_tag="VEW08_09615"
     CDS             1759..3144
                     /locus_tag="VEW08_09615"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HYJ41037.1"
                     /translation="MGSGTVFEDENGASPLADTVVASKRISIFGLGYVGAVSLACLAR
                     DGHRVIGVDIDPVKLALIRDRKSPILEEGIQELMRDVVNSGRVTVTADAAAAMRETEL
                     SFVCVGTPSAANGSQDLTAILRLAEQIGAALALKDGFHTIVIRSTVQPGTVEDKIEPI
                     LERASGKKAGVDFGVCFQPEFLREGSSIRDYDHPPYTIVGANCEAAVDQVRALFRHLD
                     TRFLVTSIRVAETLKMACNAFHALKITFANEIGRVSQSMGIDAHEVMRLVCADKRLNI
                     SPAYLKPGFAFGGSCLPKDLRALTNIAKQNDIELPMLSSLLISNRVHIDHAVDRILKL
                     GRPKVGMLGLSFKTGTDDLRESPLVLVAKRLLGEGCELRIFDPEVQLSRLLGANRSYI
                     DANIPHLGSLLCGSIEEMINPSDVILVGLQQSALNDALQVHVQPGQFVLDLVNLPNRD
                     LLRCRYEGICW"
     gene            3517..4077
                     /locus_tag="VEW08_09620"
     CDS             3517..4077
                     /locus_tag="VEW08_09620"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HYJ41038.1"
                     /translation="MSAPATVNRPPTISGQVVETVRVGESYDWQPTAADPDGDKLTFS
                     AVNLPPWASIDSTDGRIIGTPGESDVGIYESITITVADAALQSATTPFSITVLGEAGS
                     GVASLRWETPPSKVDGSPLDDLAGYRILYGRNSDDLDKSVLISNATTTSYEFSTLSSG
                     IWYFSVVAVSACGLEGPPTTIASKSI"
     gene            4160..5542
                     /locus_tag="VEW08_09625"
     CDS             4160..5542
                     /locus_tag="VEW08_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41039.1"
                     /translation="MKSLGLIGLGLAALGASTALAVPVIPGAAGFGMDTPAGRGGTVY
                     KVTNLTASGAGSLKACIDGTTARTCVFEVSGVIRLTSDLIIRNSKITIAGQTAPSPGI
                     MIRGAAIRIQASDVLIQHIRVRTGDDANGPDPDNRDSLKIEGSATKPVKNIVIDHCSF
                     SWAIDEIASAWGPTDNITFSNNIFAEPLNESIHPAYSGTGVQKHGFGVLYGSAPGSSF
                     TMVGNLMAHIVERNPLSRASEFVFVNNVVYDRGTMDLDLQSQDGVITKSSVVGNVFLR
                     GPSFSRDTRPIYVRTTGSQTLYAGSRVYTSDNYAPESTNGLISLTGGDIIPSLMQTVT
                     IPVWNTGLTARATANNAVYDRVLKFAGARPADRDVVDKRVVQSVKSRNGQVINCVAAN
                     GTTRCAKNAGGWPSYAQNRRTLTLPANPNTVASNGYTNLENWLHSMDLNVAGVVSAQS
                     PTSPPALSVQ"
     gene            5671..7104
                     /locus_tag="VEW08_09630"
     CDS             5671..7104
                     /locus_tag="VEW08_09630"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HYJ41040.1"
                     /translation="MNTEYHDQVSQPIFFKRGMSLAAPVLLWLLDALPAVMAGVMFYP
                     LTRVYDVEFDRPFLVLSILAATFTLAILPTRNPTRQVISGRLELATNLLLRWAVLVAA
                     LLALGYMTKFSEDFSRRVVVTWVLVTPVLLVMLSLILQSVTRALLRDAAQSRRAVIVG
                     CTQASMELSKRLALHVELGISVAGFFDDRGSDRLGCSRHAQLLGRFEDVAAFVNTRNI
                     DVIFVALAPGHVGRVRELVHELGDTTASIYFLPDVTGFDSIQQRASEILGMPVVAMCE
                     TPFHGYRGLIKRLMDVTIAVGALIVLAPLLAGIAIAVKRGSPGPVLFRQRRYGLDGRE
                     INVYKFRTMTVCENGALVTQVSRGDTRVTPIGKHLRRWSLDELPQLINVLQGTMSIVG
                     PRPHAVAHNEEYRKLIKRYMVRHKVLPGITGLAQVRGCRGETARVEHMEARVLFDLEY
                     MRRWSPMLDLEIILATAVAVIRTDNAY"
     gene            7165..8466
                     /locus_tag="VEW08_09635"
     CDS             7165..8466
                     /locus_tag="VEW08_09635"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HYJ41041.1"
                     /translation="MPTILGLAALFLLATDALNMDLSLMPGVSAKNLIIYLIFVVLAL
                     RMVVSRGSIMVAGQLQGAFIVQIGYAIVTWLVAALLIQYPYYNLVSSGIKLKTGLIDH
                     YIFFLVFLFGVRTTDDALKVIKGLLFGALAANIITILDAAGIISLGFRIREDGRTAGA
                     IGESNQYAAFIILFLPATIAAAVAAHGFRRLFWFGATLVGCMTLVMTASRGGFVGLAM
                     MCAVGAYLYRHLISYSRISGWVFGSLILLVLIVSFSQYGNLLAERMIGTSGSIDASTA
                     SSGRSDIWATALGVMFANPITFITGFGWDAYAYMPFQFSPHNHYLALWFNLGLVGLGF
                     GSYLLFSAIGRARRASLLAAPPLRGQLIAFVLGTVGVATAVFFVDLHKPWFYFWMYGG
                     VAMRMVLCAEKEPLTEAVTPRLKRPTVRRDPYGWAHSGTRP"
     gene            8463..9626
                     /locus_tag="VEW08_09640"
     CDS             8463..9626
                     /locus_tag="VEW08_09640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYJ41042.1"
                     /translation="MTAQRICVVGLDSYGMLSGEGNPKYIGGESIQHVLLARAWRDLG
                     HDVSIIVHDEGQGARRQIDGITAIAAHTRHGGIRGLRFFHPRATRLLSALISADANVY
                     YQSPAGAYTGITAWFCRRTGRRFIFRVASDSDCEKEHGRIQFWRDRKLYNYGLRRADL
                     VAAQTEFQAQMLRENHAIESVVVNMMVEPPRNGVPVDKDIDVLWVSNLRALKRPELAL
                     ELARQLPQVKFSIVGGPMPRGETYYEDMRAAAARLPNVNMLGAVRYADSGNLFDRAKI
                     FLNTSSIEGFPNTFLQAWIRGVPVVSFFDPDSLVQRLQLGHIASSVDEMREAIRSLLE
                     DASDRQRTGRRARDFATREFTTGVAARYLELLNQKSPQKRLSADQWAKGADLP"
     gene            9623..10846
                     /locus_tag="VEW08_09645"
     CDS             9623..10846
                     /locus_tag="VEW08_09645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYJ41043.1"
                     /translation="MTNPTCLAPHMKQPLTLVTPRATHAAAAAVATPACDVLFVLSSL
                     AVGGSERKIARLANRLREEGVAVNLACLNAPYTLEPTLRRDVPLAKLERRGKFSFATV
                     LRLRQMILRDRPATVVAVNLYQALYVACATRLLPNRPRIVALVNTSTFRGRRMRKRLY
                     QSVLARFDLVVHGSRAQSRFWETAGTSTAHEKSTVIYNGVDSACFDPVEAFEAAKRLR
                     ASLGVKPGALLLGSVGRLAPEKNHEVLLTTLRRLRVARVDAHLVIAGNGPLREQLLRR
                     AAELEIADRVNLIGEFEDVRPVLAAMDVFVLPSTAVESFSNAALEAMSMGRPVILSDI
                     GGAREMIEDGVEGYVVSATELSARLPAIIAALYADPRKRQNMGAAARARVVTRFSVAG
                     MAAAYRGLLHGEVSQ"
     gene            10843..11994
                     /locus_tag="VEW08_09650"
     CDS             10843..11994
                     /locus_tag="VEW08_09650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYJ41044.1"
                     /translation="MSADPTTVLMPRSVAPRAPSPREARMKVLQMGPSLDTRGGISSV
                     EQLICDYLPPYAAIRHVATMTEGSKLTNAAVFARAMRTLSRSLRDIDPAIVHIHFASR
                     GSTLRKLILASMVVRARRPLILHAHGAGFDQFYRGLHSPMRNLVSRVLQQASVLIALS
                     SQWRDFYVQECEVSPSQVMVLPNPVRVPSRVPDRAGRSQVQFLHLGRLGQRKGGYDLV
                     NAFAALPESLRSRARLVLAGDGDVEGMRKLAAPLGDRVRVLSWIDVHERERLLTESDV
                     YVLPSYAEGVPMSLLEAMAAGLPGITTPVGGIPDVFTHGAEGLLVPPGDAAQLRAAMS
                     IFIGDEPARLAAGKRAYERARAFDVHAYARRLADIYQRIAPVAELRETA"
     gene            11991..13376
                     /locus_tag="VEW08_09655"
     CDS             11991..13376
                     /locus_tag="VEW08_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYJ41045.1"
                     /translation="MSAVTTFAGFMKKHVLGQTLIRRNPYYYDRSSNVLERGETWEYE
                     RRRTWVGQHVRRTLQLAKRAEYGRSVRGGDTLASWPLLDKESLRHGLSAFTTGYKWLS
                     SPATTGGTSGVPLKVLRSLEAIVFEQATIDRVIRSVGVDARTARTVVLRGDNPRDIEV
                     SPNPDCEIIDGGRIMTMSANAVTHTSVEHIVNEIEKFGPQLLCAYPSALETLVRHLRD
                     NGRRLSIQAVVTSSEVFRPESWALVEEMLGCRIADYYGQAERIAFASANAAGQYRFQH
                     GYSCVEFVPYDGQRVPGDSQHRLYEIVGTSFWNSLLPIVRYRTGDLVRLPANWGEREL
                     EELSLGLRTFEGVLGRQQELLVCPQAVRITGIGCIPQDVRNVLRFQVVQEDLDEARIL
                     VLPCEKFSVNDAEVLLANARARIPSHVNVTVEIATRLERTPRGKTPLIVHRPPVHDAL
                     RRQGVEPLFTR"
     gene            13380..15374
                     /locus_tag="VEW08_09660"
     CDS             13380..15374
                     /locus_tag="VEW08_09660"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HYJ41046.1"
                     /translation="MQQPRWYLQRLKVMSGAEVAHRLARGLRVLSQKFASTETPPRDV
                     SARDLRFLPPYIAIAPEHLTAAAERVLAGKYSFFDLAECELGDPPQWNRDPLTKLAAG
                     DRRAAAIDYRDEREVGNIKYVWEPNRHLHLPVLAQAHALTGDVRYALAIQAQIDSWIE
                     QCPEGWGPNWISPLELGIRLINWSITWQLLGGMRSKLFATAEGQAFRDRWLDSIFRQT
                     RMIAGNLSRYSSANNHLIGEAAGVYVAASTWPLWPQVTEWGERCRIILEEECQRQNAP
                     DGGNREQAFGYQTFVLDFLLLAGLAARARGEDFAPVYWRRVEVMIDFLASMTCVAGRL
                     PMIGDADDGRVVSLATEPGFSAHISLIATGAVLFGRADLAAKAGALDGKTATLLGVEA
                     VRRLAQLKQRSRNGFRPRQQFSESGYYLMGSGFDTPDEVRLLVDAGSLGYLSIAAHGH
                     ADALSFVLNIGDREILVDPGTYAYHTDPAWRRYFRGTLAHNTVGVDELDQSVQAGNFM
                     WTDHAQARCIEFETGVDRQRFVGEHYGYQRLEDPVVHRREIVCDTRRQIIEVTDMLRC
                     DSEHRIRRSWHFAEDCQVERFGRGLKVTSGLTQVLFEPLEELDSMQLTRGGSAEQGGW
                     VSRSFGCKRVSTSAHWHSRVKGVTVVRTRITYTRSRDNGL"
     gene            15364..>16157
                     /locus_tag="VEW08_09665"
     CDS             15364..>16157
                     /locus_tag="VEW08_09665"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HYJ41047.1"
                     /translation="MASEPAISEVDLHQVVTADSPSGDRTHVLRQDDCFGLFNEIGDI
                     DAETRSEAGLYRGGVRHLSRLTMTIAGQRPLLLAASPREDNVLLTMNLTNADVRGEGG
                     RVVLPRGTLHITRSRFIWSGVCYELIRVRNFALSTVSAELVVRFGSDFENIIDVRAQR
                     RVDAARSRFERVERDSVIQGCLGADEIVRETVVDCHTLPEAISPDSLRYRLQIGSRGE
                     KTISLAFGCRSANLPIEIATFTRSLAAAEELARVEGRPPCQVDSTDA"
BASE COUNT         2840 a         5193 c         5131 g         2993 t
ORIGIN      
        1 gcgggaccgg atgccggcag cgttcgccag ctcgaagatc aatacagccg catgcgctcg
       61 agctacgacg agagccaccc ggacatggtg cagttgcgcc ggcagatcga cagcctgaag
      121 ttcggcacca gcgcgggcac cggtacctcc ttacgctcgc agctgaattc caagcgcgcg
      181 acgctggccg aagcccggca gcgttacggc gccgaacatc cggacatcaa gcggttgcag
      241 cgtgatatct ccactctgga gacgcgcatc aagaacggcg agcgcggcga tgtcgaaatg
      301 tccgatggca caccagtcgg aatgcagctg cgcacgcaga tcaatgccat cgacagccag
      361 atcggcagcc tcatggcgcg cagcgcggag ctgcgggcca aggtgtcgag cctcgaaaga
      421 aacgtgacgg ccgcgccgca ggtggagcgg gagtatgcga acgccacgcg cgacctgacc
      481 atcgctcgtg agaagtacga acagcttctc aaccgccaga tggacgccga ggtcagcgag
      541 gcggccatcg tcggtgggcg tgccgatgag tttcgtctca tccaggcgcc tatgctgccc
      601 ggtgcgccgg ccaagcccga acgcctcgcg atactgctga tcggcctggt tgccgccgtc
      661 gtgctcggtc tgacctccac gattgccgcc gaggctctcg atcccaaggt gcgcggtgcg
      721 cgcgatatcc gtgagctgct gtcggtatca ccgctggtcg ccatcccgga catccgcaac
      781 tcccgctcgc ggcggcgcaa tacatggcgc ttcgtcacgg ccagcgccag tggcgtgatc
      841 ggcgtgtgga tcgtcttcac cgccattgtg aattacctgt gacccaacga gagacacctc
      901 aaatgagtac gaccatggaa gatgcgatcc gccggaactt tgccgatcgg tcgggcactg
      961 agcgtcccaa caccatcgag acagcgaatc tgcggcagcg tgtgcgccgc gtgaccgctg
     1021 cacccgcaga gctgcagcag gcacgtcgct tccgccaggc cagcgtggac gcacgcgcga
     1081 tggaacgtca gtgcatcctg cccgcggtct ccgatgtcgc ggcactgcgc gcgttcaaga
     1141 tcctgcgcac gcgtgtgctg cggcgtctcg acatgcacaa gtggaacagc atcgccgtct
     1201 ccggcatgtc ggtgtgcgag ggcaagacga tgaccgcgat caacctcgct ctcgcgttgt
     1261 cgcaggacgt gaacacctgg gtgttcctgg tcgatctcga cctgcagcgc ccgcagatag
     1321 ccaactacct cggcatgagc ttcgagaagg gactgtccga ctacctgctc ggcaaggcgg
     1381 aactcgaaga cgtggtttac aacctgcagt cggagcgcct cggcgtcatt ccgaatgccc
     1441 gcgcgttcca gcattcctcc gagttcctga attcaccgcg catgggcgag ttcacggctg
     1501 cgctcgagcg cgagatgccc cgccgcatcg tgatcttcga catgccgccg ctgctggcgt
     1561 cggatgacgt gctggccttc gcgccacagt tcgattcgct gctgctggtc gtctccgaag
     1621 gcaagagtgg ccgcgccatg ctccgcggtg caaaggaaat cctcagcgag atgaacctgc
     1681 tcggtgttgc gctcaatcgt tcgaaggaaa gaaacgaaag tgcttattac taaagagacc
     1741 ggttcagcga acgaagaaat ggggtcaggt accgttttcg aggacgaaaa cggagccagt
     1801 cccctggctg ataccgtcgt cgcttcgaag agaataagca tcttcggctt gggttatgtt
     1861 ggcgccgtgt cgctggcctg cctggcgcgc gacggacacc gtgtgatcgg cgtcgacatc
     1921 gaccccgtca agctcgcgct gatccgcgac cgcaagtcgc cgatcctcga ggaggggatc
     1981 caggagctca tgcgcgacgt ggtgaattcc ggccgcgtga ccgtgacggc ggacgctgcc
     2041 gcggcgatgc gcgagaccga actctcgttc gtttgcgtgg gaacgccttc tgcggcgaat
     2101 ggcagccagg atctcaccgc cattctgcgg ctcgccgagc agatcggtgc ggcgcttgct
     2161 ctcaaggacg gattccacac catcgtcatc cgctcgacgg tgcagccggg cacggtggaa
     2221 gacaagatcg agcccatcct cgagcgtgcc agcggcaaga aggcgggcgt cgacttcggt
     2281 gtgtgctttc aacccgagtt tctgcgcgag ggatcttcga tccgcgacta cgatcatccg
     2341 ccttacacca tcgttggcgc gaactgcgaa gcggcggtgg accaggttcg cgcgttgttc
     2401 cggcacctgg acacgcgttt tctcgtgacc agcattcgcg tcgccgaaac gctcaagatg
     2461 gcctgcaacg ccttccacgc gctcaagatc actttcgcca acgagatcgg ccgcgtctcg
     2521 cagtcgatgg gcatcgatgc gcacgaagtc atgcgcctgg tgtgtgccga caagcgcctc
     2581 aatatttctc ccgcctacct gaagccgggg tttgcgttcg ggggctcgtg cctgccgaaa
     2641 gacctgcgcg cactgaccaa cattgcgaag cagaacgaca tcgagctgcc catgctctcg
     2701 agcctgctca tcagcaatcg cgtgcacatc gatcacgccg tcgacaggat cctgaagctc
     2761 ggccggccga aggtcggcat gctggggctg tcgttcaaga ccggcaccga cgatctgcgc
     2821 gagtcgccgc tggtgctcgt ggccaaacgg ctgctgggcg agggttgcga gctgcgcatc
     2881 ttcgaccccg aggtgcagtt gtccaggttg ttaggtgcca accgcagcta catcgacgcg
     2941 aacattcccc acctggggtc actgttgtgc gggagtatcg aggagatgat caatccgtcg
     3001 gacgtcatcc tggtcgggct gcaacagtcc gccctcaacg atgccttgca ggtgcacgtg
     3061 caacctgggc agtttgtcct cgatctcgtg aacctgccga atcgtgatct cctgcgctgc
     3121 cggtatgagg gcatctgctg gtagttcggt cggcgcgggt caccgtgcgc atatataaga
     3181 gaacgccgac gcggcccgat ttggcgctgt gtctcactcc tgtaaccctg taaatatttc
     3241 aacttcactc aagctgttga ttcgcttcta ttgtcgcatt ccgccgacgt aggaggagtc
     3301 gggcagaatt ccgccaatgc gttgaatcca ttgccgaatg agtcgtaaat ctgtcttcac
     3361 ccttagaggg ctgacagagg actccgacaa atggttcgac cgatacgcaa ttttgtaagc
     3421 gcgctcctac cgatactgcc gctcgccttc tgcaatggct gcggcggcgg tggctcatcg
     3481 tcggacaccg ccccacaagc cgcgtctccg ccgcccgtgt cagcacccgc aacggtgaat
     3541 cgtccgccca ccatttccgg ccaggtcgtc gagaccgtgc gcgtgggcga gagctacgac
     3601 tggcaaccca ctgccgccga tccggatggc gacaagctga ctttctcggc ggtgaacctg
     3661 ccaccgtggg ccagcatcga ttcgaccgac ggacgcatca tcggcactcc gggggaaagc
     3721 gacgtcggca tctatgaatc gatcacgatc acggttgcgg atgccgctct ccagagcgcc
     3781 accacgccgt tctccatcac ggtgctgggc gaggcgggca gcggagtcgc gtcgctgcga
     3841 tgggaaacgc caccgtcgaa agtggatggt tcgccgctcg atgatctggc gggttatcgc
     3901 attctgtatg gtcgcaacag cgacgacctg gacaagagcg tgctcatcag caacgcgacc
     3961 accacgtcat acgagttcag cacgttgtcg agcggcatct ggtacttctc cgtggtcgct
     4021 gtgagcgcct gcggtctcga aggcccaccg accaccatcg cctcgaagtc tatctgacaa
     4081 acatcttccc gttttccgag aggttccagt aatgaagctt gctagtgtcc attcccacca
     4141 tgtatccgcg cgccacattg tgaagtccct tggcctgata ggccttggcc tggccgcgct
     4201 cggcgcctcc accgcgctgg ccgtcccggt gatccccggc gcggcaggct tcggcatgga
     4261 cacgcccgcc ggccgcggcg gcacggtgta caaggtcacg aatctcacgg cgagcggcgc
     4321 gggctctctc aaggcgtgca tcgacgggac gacggcgcgc acctgcgtgt tcgaagtgtc
     4381 gggcgtcatc cgcctgactt ccgacctcat cattcgcaac agcaagatca ccatcgcggg
     4441 ccagacagcg ccgtcgccgg gcatcatgat ccgcggcgcg gccatcagga tccaggcctc
     4501 ggacgtgctc atccagcaca tccgtgttcg aaccggcgat gacgccaacg gtcccgaccc
     4561 ggacaaccgt gattcgctca agatcgaagg cagcgccacc aaaccggtga agaacatcgt
     4621 catcgaccac tgctcgttca gctgggcaat cgatgaaatc gcgagtgcct ggggcccgac
     4681 cgacaacatc acgttcagca acaacatctt tgccgagccg ctcaacgaat ccatccatcc
     4741 ggcgtactcg ggtaccggcg tgcagaaaca tggcttcggc gtgttgtatg gcagcgcccc
     4801 cggcagcagc ttcacgatgg ttggtaacct gatggcgcac atcgtggaac gcaacccgct
     4861 gtcacgcgcc agcgagttcg tgttcgtcaa caacgtggtt tacgaccgtg gaacgatgga
     4921 cctcgacctg cagagccagg acggtgtcat caccaagagt tcagtcgttg gcaacgtctt
     4981 cctgcgcggg ccgagtttct cgcgcgacac acgtccgatc tacgtgcgca ccaccggcag
     5041 ccagacgttg tacgccggca gccgcgtata cacgagcgac aactacgcgc cggaatcgac
     5101 caacggcctt atctcgctca cgggcggcga tatcatcccg agtctcatgc agacggtgac
     5161 tatcccggtc tggaacacgg gactcaccgc gcgcgccacg gcgaacaacg cggtctacga
     5221 tcgcgtgctc aagttcgcgg gtgcgcgtcc tgcggatcgc gacgtcgtcg acaagcgcgt
     5281 cgtgcagagc gtgaagagcc gcaatggaca ggtcatcaac tgcgtcgcgg ccaatggcac
     5341 cacgcgttgt gcgaagaacg cgggtggctg gccttcatat gcacagaaca ggcgcacgct
     5401 gaccttgccg gcgaatccga acacggtggc gtcgaacggc tacaccaacc tcgagaactg
     5461 gctgcactca atggacctga acgtggccgg ggttgtgtcg gcccagagtc ctacatcacc
     5521 gcctgcattg tcagtgcagt gatgattacg taagcaggag aattcggagg gcgtgtaatc
     5581 gttcggcgat agcgcatcga ggaaagggcc ggcgtcaaag cgaccggcct ttttctattc
     5641 tggacgccgt gtagggaagg gacgcgtccg ttgaacaccg aatatcacga tcaagtcagt
     5701 cagcccatct tcttcaagcg tggcatgtcg ctggccgcgc ccgttttgct gtggctgctc
     5761 gacgctttgc cggccgtgat ggcgggcgtg atgttttatc cactcacgcg cgtctacgac
     5821 gtcgagttcg atcgcccctt cctggtgctg tcaatacttg cggccacttt cacgctggcc
     5881 attcttccta cgcgcaatcc tacacgccag gtcatctccg gccgcctcga gctcgcgacc
     5941 aacctgctgt tgcgctgggc ggtgctggtg gcggcgctgc tggcgctcgg ctacatgacc
     6001 aagttctcag aagacttctc gcgtcgcgtc gtcgtgacct gggtgctggt gaccccggtg
     6061 ctgctggtca tgctgtcgct catcctgcag agcgtgacgc gcgcgctgct gcgcgatgcc
     6121 gcgcaatccc ggcgcgccgt gatcgtcggc tgcacccagg ccagcatgga gctgtcgaag
     6181 cgcctggcac tgcatgtcga gctcggcatc tccgtggcgg gattcttcga cgaccgcggc
     6241 agcgacaggc tgggctgttc caggcacgcg caattgctcg gacgcttcga agacgtggct
     6301 gcattcgtca acacgcgcaa catcgacgtg atcttcgtcg cgctggcccc gggccacgtc
     6361 gggcgcgtgc gcgaactggt gcacgagctt ggcgacacca cggcgtccat ctacttcctg
     6421 ccggatgtga cgggcttcga ctcgatccag cagcgtgcca gcgagattct cggcatgccg
     6481 gtggtggcca tgtgcgaaac gccgttccac ggttaccgcg gtctcatcaa gcggctcatg
     6541 gacgtgacca tcgccgtcgg ggcactgatt gtgttggcac cgctgctcgc gggcatcgcc
     6601 attgcggtga agcgcggatc gccgggccct gtcttattcc ggcagcgtcg ctacggcctc
     6661 gacggacgcg aaatcaatgt ctacaagttt cgcaccatga cggtgtgcga aaacggcgca
     6721 ctcgtgactc aggtgtcgcg cggcgacacc cgcgtgacgc cgatcggcaa gcatctgcgg
     6781 cgatggtcgc tcgacgagct gccgcagttg atcaatgttc tgcaggggac gatgagcatc
     6841 gtaggaccgc gcccacatgc ggtcgcgcac aacgaggaat accgcaagct catcaaacgg
     6901 tacatggtgc gacacaaggt tctgccgggc attacgggat tggcgcaggt gcgcggttgc
     6961 cgaggtgaga cggcccgggt ggaacatatg gaagctcgcg tgttgttcga tctggagtac
     7021 atgcgtcggt ggtcaccgat gctggatctg gaaatcatcc tggccacggc tgtcgccgtg
     7081 atcaggaccg acaacgcgta ctgaagagct gccgcaggaa aaagctctac taccgtttta
     7141 attgttttcg gacgactctg acgcatgccg acaattctcg gactcgccgc actgttcctg
     7201 ttggcaaccg acgcgctcaa catggacctg agtctcatgc ccggcgtgtc ggcgaagaac
     7261 ctcatcatct acctgatctt cgtggtgctg gcgctgcgca tggtggtcag ccgcggctcc
     7321 atcatggtcg ccggccagtt gcagggtgcg ttcatcgtgc agatcggcta cgccatcgtc
     7381 acgtggctcg tcgccgcgct gctcatccag tatccgtact acaacctggt gtcgagcggc
     7441 atcaagctca agaccggtct catcgaccac tacatcttct tcctcgtgtt cctgttcggt
     7501 gtgcggacca ccgacgatgc gttgaaggtc atcaagggcc tgctcttcgg cgcactggcc
     7561 gccaacatca tcactattct cgacgccgcc gggatcatca gcctggggtt ccggatccgc
     7621 gaggacggac gcaccgcggg cgccatcggc gagtcgaatc aatacgcagc tttcatcatc
     7681 ctgttcctgc cggcgaccat tgccgcggcc gtggcggccc atggattccg gcggctgttc
     7741 tggtttgggg ccaccctggt cggctgcatg acgctggtca tgaccgcatc gcgcggcggc
     7801 ttcgtcggtc tcgccatgat gtgcgcagtg ggcgcctatc tataccgcca cctcatttcc
     7861 tacagccgca tctccggttg ggtgttcggc tcgctgatcc tgctggtgct gatcgtttcg
     7921 ttctcgcaat acggcaacct gctcgccgag cgcatgattg gaacatcggg atccatcgac
     7981 gccagcacgg cgtcttcggg acgcagcgac atctgggcga ctgccctggg cgtcatgttc
     8041 gcgaacccca tcacattcat caccggcttc ggatgggatg cgtacgcgta catgcccttc
     8101 cagttctcac cgcacaatca ctatctcgcg ctgtggttca acctgggcct ggtcggcctg
     8161 ggcttcgggt cctatctgct gttctcggcg atcggccgag ctcgacgcgc gagtctgctg
     8221 gccgcgccac cgctgcgtgg ccagctcatc gctttcgttc tcggcaccgt cggtgtggcc
     8281 accgccgtgt tcttcgtgga cctgcacaag ccgtggttct atttctggat gtacggcggc
     8341 gtggccatgc gcatggtgct gtgcgcggag aaggagcccc tcaccgaggc ggtaacgccg
     8401 cgtttgaagc gtccgacggt gcggcgcgat ccgtacggct gggcgcactc ggggacgcgc
     8461 ccatgactgc gcagcggatc tgcgtcgtcg gcctcgattc atacggcatg ctgtcgggcg
     8521 aaggaaatcc gaaatacatc ggcggcgagt ccattcagca cgtgttgctg gcgcgcgcgt
     8581 ggcgtgatct cgggcatgac gtctcgatca tcgtgcacga cgaggggcag ggcgcgcgcc
     8641 gtcagatcga tggcatcacc gcgatcgccg cgcatacgcg ccatggcgga atccgcgggc
     8701 tgcgattctt ccatccacgc gccacgcggc tcttgtcggc gctgatttcc gccgacgcga
     8761 atgtctacta ccagtctccc gccggtgcct acaccggcat cactgcctgg ttttgccggc
     8821 gcaccggacg gcgattcatt ttccgcgttg cttccgacag cgattgcgaa aaggagcacg
     8881 gccgcatcca gttctggcgc gatcgcaagc tctacaacta cggactccgc cgcgccgacc
     8941 tggtggcggc gcagaccgag ttccaggcgc agatgctgcg cgagaatcat gcaatcgaga
     9001 gcgtcgtggt caacatgatg gtcgagccgc cgcgcaatgg cgtgccggtc gacaaggaca
     9061 tcgacgtgtt gtgggtcagc aaccttcgcg cgctcaagcg gccggagctc gccctggagc
     9121 ttgcacggca gctgccgcag gtgaagtttt cgatcgtcgg tggcccgatg ccgcgtggtg
     9181 aaacatatta cgaggacatg cgcgctgcag cagcacggct gccgaacgtg aacatgctgg
     9241 gagcggtgcg ttacgcagat agcggaaatc tgttcgaccg cgcgaaaatc tttctgaaca
     9301 cgtcgagcat cgagggattt cccaacacct ttctgcaagc gtggattcgc ggcgttccgg
     9361 tggtgtcgtt cttcgatccg gattcgctgg tgcaacgcct gcagctcggt cacatcgcca
     9421 gctcggtcga cgaaatgcgt gaagccattc gcagcctgct cgaagacgcc tccgaccgcc
     9481 agcggaccgg gcgccgcgct cgcgatttcg cgacgcgcga attcaccacc ggggtcgccg
     9541 cccgctatct ggaactactg aatcagaaat cgccacagaa gcggctgagc gccgatcaat
     9601 gggcgaaggg agctgatctg ccatgaccaa cccgacctgc ctcgcacctc atatgaagca
     9661 accgctgaca ctcgtgactc cgcgtgccac acacgcggcg gccgctgccg tggccacgcc
     9721 agcctgcgac gtgctgttcg tgctcagcag cctcgcagtg ggtggctccg aacgcaagat
     9781 cgcgcgcctg gccaaccgcc tcagggaaga gggcgttgcc gtaaacctgg cctgtctcaa
     9841 tgcgccgtac acactcgagc cgactctgcg acgggacgtg ccgctggcca agctcgagcg
     9901 ccgcggaaag ttctcgttcg caacggtgct gcgcctgcgg cagatgatcc tgcgcgaccg
     9961 gccggcgacg gtggtcgcgg tgaacctgta ccaggcgctg tacgtggcct gcgccacgcg
    10021 gctgctgccg aatcggccgc gaatcgtggc gctggtgaac acctcgacgt tccgcggccg
    10081 gcgtatgcgc aagcggctct accaatccgt gctggcgcgc ttcgacctgg tggtgcacgg
    10141 ctcacgggcg caaagccgtt tctgggaaac cgcgggcacc tcgacggccc acgaaaaatc
    10201 cacggtcatc tacaacggcg tggattccgc ctgcttcgat ccggtcgagg cctttgaagc
    10261 agcgaagcgc ctgcgcgcat cgctcggagt caaaccggga gcgctgctgc ttggcagcgt
    10321 gggccggctg gcccccgaga agaaccatga agtgctgctg acgacactgc gccgcctgcg
    10381 ggttgcgcgc gtcgatgcgc acctggtcat cgccggcaac ggcccgctgc gcgaacagct
    10441 gctgcggcgc gcggcagagc tggaaatcgc cgaccgcgtg aacctcatcg gcgagttcga
    10501 agacgtgcgg ccggtgctgg ccgcgatgga tgtattcgta ttgccgtcca ctgccgtgga
    10561 atcgttttcg aatgccgcgc tcgaggccat gtccatgggg cgtccggtga tcctgtcgga
    10621 cattggcggc gcgcgcgaga tgatcgaaga cggcgtcgag ggttacgtgg tctcggccac
    10681 ggaattgagc gcgaggctgc cggcaataat cgccgcgctg tacgccgacc cacgcaagcg
    10741 gcagaacatg ggagcggcgg cgcgagcccg ggtcgtcacc cggttttcgg tggctggcat
    10801 ggcggcggcg taccgcgggc tcctgcacgg cgaggtgtcg caatgagcgc cgatcccacc
    10861 accgtcctca tgcccaggtc tgttgcgccg cgtgcgccat cgccgcgcga agcgcgaatg
    10921 aaggtattgc agatggggcc gtcgctcgac acgcgcggcg gcatcagctc ggtcgaacag
    10981 ctcatctgcg actatctacc gccgtacgcg gccattcgac acgtggccac catgaccgaa
    11041 ggctcgaagc tcacgaacgc cgcggtgttc gcgcgggcga tgcgcacctt gtcccgctcg
    11101 ctccgggata tcgatccggc catcgtgcac atccacttcg catcgcgcgg cagcacgctg
    11161 cgcaaattga ttctcgccag catggtggtg cgtgcacggc gtccgctgat cctgcacgcg
    11221 catggcgcgg gcttcgacca gttttatcgc ggtctgcact cgccaatgcg caacctcgtg
    11281 agccgcgtgc tgcagcaggc cagcgtgctg attgcgttgt cgagtcagtg gcgcgacttc
    11341 tacgtgcagg aatgcgaagt gtcgccgtcg caggtgatgg tgctgccgaa tccggtgcgc
    11401 gtaccgtcgc gggtgccgga tcgcgccggg cggtcgcagg ttcagttcct tcatctcggg
    11461 cgactcgggc agcgcaaagg cggctatgac ctcgtgaacg cctttgcggc gctgccggag
    11521 tcgctgcgtt cccgtgcgcg cctggtgctg gccggcgatg gtgacgtcga gggcatgcga
    11581 aagctggcgg cgcccctcgg cgaccgcgtg cgtgtgctgt cgtggatcga cgtgcacgag
    11641 cgagaacggc tgctcaccga aagcgatgtg tacgtgctgc cgtcttacgc cgaaggtgtg
    11701 ccgatgtccc tgctcgaggc gatggcggcg ggactgcctg gcatcacgac accggtgggc
    11761 ggcattcccg acgtgttcac tcacggcgct gaaggattgc tggtgccgcc cggcgacgcg
    11821 gctcagttgc gcgcggcgat gtccatcttc atcggcgacg agccagcgcg cctcgcggcc
    11881 ggcaagcgtg catatgaacg cgcgcgtgcc ttcgatgtgc acgcgtatgc gcggcgactc
    11941 gcggatatct accagcgcat cgcgccggtg gcggaattgc gggagacggc atgagcgcgg
    12001 tcaccacatt cgccgggttc atgaaaaagc acgtgctcgg gcagaccctg attcgccgca
    12061 atccttacta ctatgaccgc tcatcgaacg tgctcgagcg gggtgagacc tgggaatacg
    12121 agcggagacg cacctgggtt ggccagcacg tgcggcgcac gctgcagctc gcgaaacgcg
    12181 ctgaatacgg gcgttcggtg cgtggtggcg acacgctagc gagctggccg ctgctcgaca
    12241 aggaaagcct gcggcacggt ctctcggcat tcaccacggg atacaaatgg ctctcatcgc
    12301 ccgcgaccac gggtgggacc agtggcgtac ccctcaaggt cttgcgatcg ctcgaagcca
    12361 tcgtcttcga gcaggcgacc atcgatcggg tgatccggtc ggtcggcgtc gatgcgcgca
    12421 cggcgcgcac cgtggtgctg cgcggcgaca atccgcgcga catagaagtt tcacccaacc
    12481 ctgattgcga gatcatcgac ggcggtcgca tcatgaccat gagcgcgaat gcggtgactc
    12541 acaccagcgt cgagcacatc gtcaatgaga tcgagaagtt tggcccgcaa ttgttgtgcg
    12601 cgtatccgag cgccctcgag accctggtgc gccacctgcg tgacaacggc cggcggctgt
    12661 caatccaggc ggtggtcact tcgtcggaag tattccggcc ggagtcatgg gcgttggtgg
    12721 aggagatgct gggctgccgg atcgcggact attacggaca ggcggaacgc atcgccttcg
    12781 cgtccgccaa tgcggccggc cagtaccgct tccagcacgg ctattcatgc gtcgaattcg
    12841 taccgtacga cggtcagcgc gtgccgggcg acagccagca tcggctgtac gaaatcgtcg
    12901 ggacgtcgtt ctggaacagc ctgctgccca tcgtgcgcta ccgcaccggc gatctcgtgc
    12961 gcctgcctgc gaactggggc gagcgcgaac tcgaagagct gtcactcggg ctgcgcacgt
    13021 tcgaaggtgt cctcggccgt cagcaggaac tgctggtgtg cccgcaggcg gtgcgcatca
    13081 ccggcatcgg gtgcattccc caggacgtcc gcaacgtgct gcgcttccag gtcgttcagg
    13141 aggatctcga tgaggcgcgc atcctcgtgc tgccctgcga gaagttttcc gtgaacgacg
    13201 cggaggtgct cctcgccaac gcccgcgcga gaattccgtc gcacgtgaac gtgacggtgg
    13261 aaatcgcgac acgcctcgag cgcacgcccc gcggcaaaac gccgctcatc gtgcaccggc
    13321 cgccagtcca cgatgcgctt cgccgccagg gagtagagcc gctcttcacg cggtgatgca
    13381 tgcaacaacc ccgatggtat ttgcagcgtc tcaaagtgat gtcgggcgcc gaggtagcgc
    13441 accggctggc gcgcggcctg cgcgtgctgt cgcagaaatt cgcctcgacc gaaacgccgc
    13501 cgcgcgacgt gtcggcgcgc gacctccggt tcctgccgcc gtacatcgcc attgctccgg
    13561 aacacctgac ggccgccgcg gaacgcgtac tcgcgggcaa atacagcttt ttcgatctcg
    13621 cggaatgcga gctcggtgat ccacctcaat ggaaccgcga cccgctgacg aaactcgccg
    13681 cgggcgatcg tcgtgccgcc gcgatcgact accgcgacga acgcgaggtc ggcaacatca
    13741 aatacgtctg ggagccgaat cgccacctgc acctgccagt gctcgcgcag gcgcatgcac
    13801 tcaccggtga tgtgcgttac gcactcgcca tccaggcgca gatcgattcg tggatcgagc
    13861 agtgccccga aggctggggg ccaaactgga tcagtccgct ggagctcggc atccggctca
    13921 tcaactggag catcacctgg caactgctgg gtggcatgcg ctcgaagctg ttcgcgacgg
    13981 ccgaaggcca ggcattccgt gaccgctggc tggattccat cttccgccag acccgcatga
    14041 tcgccggcaa cctgtcgcgt tattcgtcgg ccaacaatca tctgattggc gaggccgcag
    14101 gcgtatacgt ggcggcctcc acgtggccgc tgtggccgca ggtcaccgag tggggcgagc
    14161 gttgtcgaat catcctcgaa gaggaatgcc agcggcagaa cgcgcccgat ggcggcaatc
    14221 gggaacaggc cttcggatat cagacgttcg tgctggattt tctgctgctt gcgggcctcg
    14281 cggcgcgcgc gcgcggtgag gacttcgcac ctgtctactg gcggcgcgtc gaagtcatga
    14341 tcgacttcct cgcgtccatg acctgcgtgg caggcagact gccgatgatc ggtgacgccg
    14401 acgatgggcg cgtcgtgagt ctcgcgaccg agccgggttt ctcggcgcac atttccctga
    14461 tcgccaccgg tgcagtgctg ttcgggcgcg ccgatcttgc cgccaaggcc ggcgcactcg
    14521 acggcaagac cgctaccttg ttaggcgtcg aagccgtccg ccggctggca cagctcaagc
    14581 agcgatcgcg gaacggcttc cggccgcggc agcagttctc cgagtcgggt tactacctga
    14641 tgggctcggg gttcgataca cccgacgaag tgcggctcct ggtggatgcc ggctccctcg
    14701 gttatctcag catcgcggcg catggtcacg cggatgcgct gtcgttcgtg ctgaacatcg
    14761 gcgatcgcga aatcctcgtc gatccgggga cctatgccta tcacaccgat ccggcgtggc
    14821 ggcgttactt ccgcggcacg ctggcgcaca acaccgttgg cgtcgacgag ctggatcagt
    14881 cggtacaggc cggcaatttc atgtggaccg accatgcgca ggcgcgctgc atcgaattcg
    14941 agaccggcgt cgatcggcaa cgattcgtcg gcgaacacta cggataccag cgcctcgaag
    15001 atccggtggt tcaccggcgc gagatcgtct gcgacacgcg ccgccagatc atcgaagtca
    15061 ccgacatgtt gcgctgtgac agcgaacatc gcatccgccg ctcctggcat ttcgcggaag
    15121 actgccaggt cgagcggttc ggccgcggcc tcaaggtcac ttccgggctc acccaggtct
    15181 tattcgagcc gctcgaagaa ctcgacagca tgcaactgac ccgcggcggg agcgccgaac
    15241 agggaggctg ggtatcacgg agcttcgggt gcaagagggt cagtaccagc gcgcattggc
    15301 attcgcgcgt gaaaggggtc actgtggttc gcacccgtat cacctacacg aggtctcgcg
    15361 acaatggcct ctgaacccgc catttcagaa gtcgatctgc accaggtcgt gaccgccgac
    15421 agcccgagcg gcgaccgcac tcatgtcctg cggcaggatg actgcttcgg cctcttcaac
    15481 gagatcggtg acatcgacgc cgagacgcgc agcgaagcag gtttgtatcg cggcggtgta
    15541 cggcatctct cgcgcctcac gatgacgatt gccgggcagc ggcccttgct gctcgccgcc
    15601 tcgccacgcg aagacaacgt ccttctcacg atgaacctca ccaacgccga cgtgcgcggc
    15661 gaaggcgggc gtgtcgtgct gccgcggggc acgctgcaca tcacgcgctc gcgcttcatc
    15721 tggtcgggcg tctgctacga gttgatccgg gtgcgcaatt tcgcgttgag caccgtatcg
    15781 gcggagctcg tcgtgcgctt cggctccgat ttcgagaaca tcatcgacgt gcgcgcgcag
    15841 cgccgcgtcg acgccgcgcg ctcgcggttc gagcgtgtgg agcgggacag cgtgatccag
    15901 ggatgcctcg gagcggacga aatcgtgcgc gagaccgtgg tggactgcca cacgctgccc
    15961 gaggcgattt cccccgacag cctgcggtac cggctgcaga ttggcagccg tggcgagaag
    16021 accatttcgc tggcgttcgg ctgccggtcg gcgaaccttc cgatcgagat cgccaccttc
    16081 acgcggtcgc tggccgcggc cgaagaattg gcgcgcgtcg agggccggcc gccgtgccag
    16141 gtggattcga ccgacgc
//
LOCUS       DASQJS010000238         7354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2648603, whole genome shotgun sequence.
ACCESSION   DASQJS010000238 DASQJS010000000
VERSION     DASQJS010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2648603"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VEW08_09670"
     CDS             <1..1084
                     /locus_tag="VEW08_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ41048.1"
                     /translation="LEISDIDPRSLLKGAQRPAAQPEAKRPDLRVVQTDRLPKLELKL
                     EGDPIPELIQDEPPAEEVALEPMYADDVSGEEVTTEVEALAEADSDADMNVQPEFVDE
                     APQLTAGAGQFEFYGQIFELHAQQLVKLKGNGGTRRFEILVRDAHADNEFGVAPDRVL
                     QDVHDPSANSDLDRIVVEHLLIWMDENRPSWEGEPSSFSVNVGMGTIADPKFASFVAA
                     SLKNHKIPAKALGFEIPEKSCVDQVRDVDMFVQACEKIGCHVVLDDFTFHHNAIRWLG
                     SAALKFIKLDPKITAVAMKERVPQALVVAIAQASKVLGLSCVAKRVDTPAVRDWLSAV
                     GLDYAQGFLLDQPRALTSLAAASAKK"
     gene            1152..2237
                     /locus_tag="VEW08_09675"
     CDS             1152..2237
                     /locus_tag="VEW08_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYJ41049.1"
                     /translation="MPSLDPLLKNRVPLFWTLQIGGWLAYGLSQFLGTLLYDTKYENM
                     KGYVTVILIASVSGFLLSLELRYIYHRLWTRSPRLIIAVALLSCYLFALVWRVIINST
                     YVMFTSEAMEWVMKSPLEFFSNAMHSTYLLLCWTGIYFGIKYYESAQQEREATLRAAA
                     LAQEAQLKMLRYQLNPHFLFNTLNAISTLILDNENRTANQTVMRLSEFLRYTLDQDPM
                     KKVTLRQEVEAMNLYLTTERLRFGERLKLEFAIEERALEALVPSLLLQPLIENAVKYA
                     VSPSEQGGTIRVEGRARGAMLELAVADDGPGLNTGAAPGAGRGVGLRNIRERLAVLYE
                     DSHRFATLDNKPGLRVELGFPLEMAAP"
     gene            2242..3060
                     /locus_tag="VEW08_09680"
     CDS             2242..3060
                     /locus_tag="VEW08_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HYJ41050.1"
                     /translation="MTTPRKLRTLLVDDEALSRRGLQLRLALANDVEVIGECSNGREA
                     LEAIREHRPDLVFLDIQMPGLSGFDVLAELQPHELPMIVFVTAYDRFAVQAFEARAID
                     YVLKPVDDARLAATLTHVRELTEQRTAVAERDQLVNLLAELRGSGEIADSTHAGGEQA
                     PHWLPIRNGRETVRVPVQAIEWVDAAGDYLCIHADGHTHILRATMREMENLLDPRLFQ
                     RVHRSTIVNLTRVKSLRAHMNGEYFLRLEGGQELKLSRTYRDKVEYFLKRPRSH"
     gene            complement(3063..4112)
                     /locus_tag="VEW08_09685"
     CDS             complement(3063..4112)
                     /locus_tag="VEW08_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ41051.1"
                     /translation="MKLNRACIVLASALVALVPAMAPALAADAPPVPVVVTTARSQNV
                     GASLAATGTVVSRNDARISAEVAGTLAWIAEPGAAVKRGATIARIDPERLALTLRDND
                     AALKRLEAQLSLLATQRDRLQTLGNVVSQSQLDEALSRERMAEQDVEQARVARDRARL
                     DVNRASVRAPFDGIVAERLQQSGEYVAGGAPLLRLVNDQDLEVVARAPLTTADSVTAG
                     SVAQLIDGDRKASGTVRAVIPVGDERSRMVELRIALTDRTWRVGAPVRVEIAPRSLKA
                     VVTVPRDAVILRQGASYVMRVRPDNTAERVAVNIGAGYANHVEIDGTLRAGDRVIIRG
                     GERLEPGQTVKVQLL"
     gene            complement(4109..7201)
                     /locus_tag="VEW08_09690"
     CDS             complement(4109..7201)
                     /locus_tag="VEW08_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYJ41052.1"
                     /translation="MNLTETSLRNPAGVLVAILMVALLGIFALVKLPIQLFPNIEEPV
                     ISIFTQWRAAAPTEIESEIVEPQERALQGIRGLRSLNAFANAGSSFINLQFAVGTEMQ
                     ATMLEVISRMNQLPPLPRDAQAPQISLGEDGGNGPNNTLSWFFVQLLPGTPGPIDNYR
                     RKIEELFRSRIESIPGVSNVRINFGSDEELQIVFDPARAAELGIQIPRIAQVAGSADD
                     VSGGFVDIGRRQYTMRFAGRYSMDQFSELVLEWRDGRPIRLGDIATIQVKRGDRTDLA
                     LQNGNPAIGIQIMKENDANVLETLNAVKAEIFTLRDNELKTMGLSIAQSFDPSVFIGQ
                     AIDMVTGNLFAGIVLAVLVLWYFVRDRRATLLVGLSIPICLTVTLVMLYAFGRTLNVI
                     SLAGLAFGVGQALDTAIVMLEAIAQQRERGLARMEAALAGAKQVWPALLASTVTAVIV
                     FLPIVFMRDAVSQLFADLALTISIAVIASLIVAVTALPLAASRWLNTENVHKGSNDRY
                     AAIAGWIVRWTDSAKRRYITVAVLFLAPVVLTWALLPPLDYLPPVKRDAIDGFIQQPP
                     GTNIDTIEREFVQPFAKRLAPYMAGEKEPALKNYYVLAGPFGTSIGVRPLDPDNIDEL
                     GRLINEEILVGFPDTQAFARQGNLFGGFGDGRNIDFQLQATDFPALLAAARFAEKLIQ
                     EKMPGAQVQAFQGLEMAEPELRLNPDDRRLNEVGWSRVELGTVVRALGDGMFVGEYFD
                     GERRLDMILRASKWDSPEALAETPVATPLGNVMPLSELVQVESTVSAGGLRRVDGHRT
                     IGLNVSPPKELSLSQAIEVLKAEVEPQVRAELPADGSVNYEGNAGSLREALSNMAQNL
                     AIALMALFLLLAALFRSVKDSLYVILTIPLASFGGVLALVILRLFTPQTLDLLTMVGF
                     VVLMGLVVNNAILLVDQARNEMRGGATVRAAVEASLATRTRPIMLTTLTTLFGMLPLV
                     LIPGPGSVLYRGLGTVLVGGMAVNSVFTLLLLPTLLRLIEKPDTQIVAVTDKQPLEAV
                     "
BASE COUNT         1351 a         2372 c         2380 g         1251 t
ORIGIN      
        1 tctcgaaatt tccgacatcg atccgcggtc attgctcaaa ggcgcgcagc gcccggcggc
       61 gcagcccgaa gcgaagcgtc ccgacctgcg agtcgtgcag accgatcgcc tgccgaagct
      121 cgagctgaaa ctcgaaggcg atccgatccc cgagttgatc caggacgagc cgcccgcgga
      181 agaagtcgcg ctcgaaccca tgtacgcgga cgacgtcagc ggcgaagaag tcaccactga
      241 agtcgaagcg ctggccgaag ccgattcgga cgccgacatg aacgtacagc cggaattcgt
      301 cgacgaagcg ccgcagctca ctgccggcgc cggccagttc gagttctacg gccagatttt
      361 cgagctgcac gcgcagcagc tcgtgaagct caagggcaac ggcggaacgc gccgtttcga
      421 aatcctcgtg cgcgatgcgc acgccgacaa tgaattcggc gtggcgcccg accgcgtcct
      481 gcaggacgtg cacgacccga gcgccaattc agacctcgac cggatcgtcg tcgaacacct
      541 gctcatctgg atggatgaaa accgcccctc ctgggaaggc gagccctcca gcttctccgt
      601 gaacgtgggc atgggcacga tcgccgaccc caagttcgcg agcttcgtgg cggcctcgct
      661 caagaatcac aagatccccg cgaaggcgct ggggttcgag atccccgaga aatcctgcgt
      721 ggatcaggtt cgcgacgtcg acatgttcgt gcaggcctgc gagaagatcg gctgccacgt
      781 ggtgctcgac gacttcacgt tccaccacaa cgcgatccgg tggttgggtt ccgcggcgct
      841 caagttcatc aagctcgatc ccaagatcac cgccgtggcg atgaaagaac gcgtgccgca
      901 ggcgctggtc gtggcgatcg cacaggcttc gaaagtgctc ggcctttcat gtgtcgccaa
      961 gcgtgtcgat acgccggcgg tacgcgactg gctttcggcc gtgggcctcg attacgcgca
     1021 gggattcctg ctcgatcagc cgcgagccct gacctcgctg gccgcggctt cggccaagaa
     1081 ataacgacca cccgagcacg ccgcgcgcca gggcgtttgt ccgccaaggc cggctcccgt
     1141 acctttgtcg catgcccagc ctcgatccgc tgctgaaaaa ccgcgttccg ctgttctgga
     1201 cgctgcagat cggcggctgg ctggcatacg ggttgtcgca gttcctcggc acgctcttgt
     1261 acgacaccaa gtacgagaac atgaagggct acgtgacggt gatcctgatc gccagcgtca
     1321 gcggtttcct gttgagcctc gagctgcgct acatctacca ccgactgtgg acgcgctcgc
     1381 cgcgcctcat catcgccgtc gccctgctca gttgttatct gttcgcgctc gtctggcgcg
     1441 tgatcatcaa ctcgacgtac gtcatgttca cgagcgaggc gatggaatgg gtcatgaagt
     1501 cgccgctcga gttcttcagc aatgcgatgc actcgaccta tctgctgttg tgctggaccg
     1561 gcatctattt cggcatcaaa tattatgagt cggcccagca ggagcgcgag gcgactctgc
     1621 gcgccgcggc gctggcgcag gaagcgcagc tcaagatgtt gcgttaccag ctcaaccccc
     1681 atttcctctt caatacgctg aacgccatct cgacgctcat tctcgacaac gagaaccgca
     1741 cggcgaacca gactgtcatg cgcctgtcgg agtttctgcg atacacgctc gatcaggacc
     1801 ccatgaaaaa ggtgaccctg cgccaggagg tcgaggcgat gaacctgtac ctcacgactg
     1861 agcgtctccg gttcggcgag cgcctgaagc tcgaattcgc catcgaagaa cgcgcgctgg
     1921 aagcgctggt gccgagcctg ttgttgcagc cgctgatcga aaacgcggtg aagtacgcgg
     1981 tatcgccatc cgaacagggc ggcacgatcc gcgtggaagg acgcgcgcgc ggcgccatgc
     2041 tggaactcgc cgtggccgat gacggcccgg gactcaacac cggagcggca ccgggtgcgg
     2101 gccgcggtgt cggtctgcga aacattcgcg agcggctggc ggtgttgtac gaggacagcc
     2161 accggttcgc gaccctggac aacaaacccg ggttgcgcgt cgagctcgga tttccccttg
     2221 aaatggcagc accctgaacg catgactacg ccacgcaaac tccgtacttt gttggtggac
     2281 gacgaagcgc tgtcgcgacg cgggctgcaa ctgcgcttgg cgctggcgaa cgacgtcgaa
     2341 gtgatcggcg agtgcagcaa cggccgcgag gcgctcgaag cgattcgcga gcaccgcccg
     2401 gacctcgtgt tcctcgacat ccagatgccc gggctctccg gcttcgatgt actcgccgag
     2461 ctgcagccgc acgagctgcc gatgatcgtc ttcgtgacgg cgtacgaccg attcgcggtg
     2521 caggcattcg aagcgcgcgc catcgactac gtcctgaagc cggtcgacga tgcgcggctt
     2581 gccgccacgc tgacccatgt gcgcgagctc accgagcagc gcaccgcggt agcggagcgc
     2641 gatcagctgg tgaacctgct ggccgaatta cgcggcagcg gggaaatcgc ggacagcaca
     2701 cacgccggcg gcgaacaagc tccgcactgg ctgccgatcc gcaatggacg cgagaccgtg
     2761 cgcgtgccgg tacaggcaat cgagtgggtg gacgccgcgg gcgattacct ctgcattcac
     2821 gccgatggcc acacacacat tctcagggcc accatgcggg agatggaaaa cctgctcgac
     2881 ccgcggctgt tccagcgcgt gcaccgctcc acgatcgtga atctcacgcg cgtgaagagc
     2941 ctgcgtgcgc acatgaacgg cgaatatttc ctgcggctcg agggcgggca ggagctcaag
     3001 ctgtcgcgca cttatcgcga caaggtcgaa tacttcctca agcggccgcg ttcgcactga
     3061 ccctataaca actgaacctt cacggtttgc ccaggctcga gccgttcacc gccgcgaatg
     3121 atgacgcggt cgccggcgcg caacgtgccg tctatctcca cgtgattggc gtagcctgct
     3181 ccaatattca ccgccacgcg ttcggcggtg ttgtcgggcc gcacccgcat cacgtagctg
     3241 gcgccctggc gcaggatgac ggcgtcgcgc ggtacggtga ccacggcctt gagacttctc
     3301 ggcgcgattt ccacgcgcac aggtgcgcct acgcgccagg tgcggtcggt gagcgcgatg
     3361 cgcagctcga ccatgcggga acgctcgtcg ccgacgggaa tcaccgcgcg cactgtgccg
     3421 gacgccttgc gatcgccgtc gatgagctgc gccacggaac cggcggtgac actgtcggcg
     3481 gtggtcagcg gcgccctggc taccacttcc agatcctggt cgttcaccag gcgcagcagc
     3541 ggtgcgccgc cggccacgta ttcgccggat tgctgcagcc gttcggcgac gatgccgtcg
     3601 aatggcgcgc gcaccgaggc gcgattcaca tcgaggcgcg cgcgatcgcg cgcgacgcgc
     3661 gcctgctcca cgtcctgctc ggccatgcgc tcacgcgaca gcgcttcgtc cagctggctc
     3721 tgcgaaacca cgttgcccag tgtctgcagc cgatcgcgct gagtggctaa caaggagagc
     3781 tgcgcttcga gccgcttgag cgctgcgtca ttgtcccgca gcgtgagcgc gagccgctcg
     3841 ggatcgatgc gcgcgatggt ggcgccgcgt ttcaccgccg cacctggttc ggcgatccac
     3901 gccagcgtgc ccgcgacttc tgccgagata cgcgcatcgt tgcggctgac caccgtgccg
     3961 gtggccgcaa ggctggcgcc cacgttttgc gaacgcgccg tggtgaccac caccggcacc
     4021 ggcggcgcat cggcggccaa ggccggtgcc atggcaggca cgagcgcgac cagcgcgctg
     4081 gcaagaacga tgcaggcgcg gttgagtttc atacggcttc caggggttgt ttgtcggtca
     4141 ccgccacaat ctgcgtgtcg ggtttctcta tgaggcgcag cagcgtcggg agcagcagca
     4201 gcgtgaacac cgagttcacc gccatgccgc ccaccagcac ggtgccgaga ccgcgataca
     4261 acacgcttcc gggaccaggg atcagcacca gcggcaacat gccgaagagc gtggtgagcg
     4321 tggtcagcat gatgggacgg gtgcgtgtgg cgagcgatgc ctcgaccgcg gcgcgcaccg
     4381 tggcgccacc gcgcatctcg ttacgggcct ggtcgaccag caggatggcg ttgttgacca
     4441 ccaggcccat gagaaccacg aaacccacca tggtcagcag atcgagcgtc tgcggcgtga
     4501 acaggcgcag tatcaccagc gccagcacgc cgccgaagct cgccagcgga atggtgagga
     4561 tcacgtagag actgtccttg accgaacgga acaacgccgc cagcagcagg aacagcgcca
     4621 tcagcgcaat ggcgaggttt tgcgccatgt tcgacagggc ttcgcgcaac gacccggcat
     4681 tgccttcgta attcaccgac ccgtcggcgg gcagctcggc ccgcacctgg ggttcaacct
     4741 ctgccttgag gacttcgatg gcctggctca acgagagctc tttgggcggg ctcacattga
     4801 gaccgatggt ccgatgtccg tcgacgcgcc gcaggccgcc agcgctcacc gtggactcga
     4861 cctgcaccag ttcgctcaag ggcatgacgt tacccaaagg agtcgccact ggagtctcgg
     4921 caagggcctc cggcgagtcc cacttgctgg cgcgcagaat catgtcgagg cgccgttcgc
     4981 cgtcgaagta ttcgccgacg aacatgccgt cgcccagcgc gcgcaccacc gtgcccagtt
     5041 caacgcgcga ccagccaacc tcgttgagcc ggcgatcgtc cgggttgagg cgcagctcgg
     5101 gctcggccat ctccagaccc tggaaagcct gtacctgggc gcctggcatc ttttcctgga
     5161 tgagcttctc ggcaaaacgg gccgcggcca gcaaggccgg gaagtcggtg gcctggagct
     5221 ggaagtcgat gttgcgtccg tcgccaaagc cgccgaacag atttccctgc ctcgcgaacg
     5281 cctgggtatc cggaaacccg acgagaattt cctcgttgat gagccggccc agttcatcga
     5341 tgttgtccgg gtcaagcggg cgcacgccga tgctggtgcc gaatggaccc gcaagcacgt
     5401 agtagttctt gagcgcgggt tccttctcgc ctgccatgta gggcgccagg cgtttggcga
     5461 acggttgcac gaactcccgc tcgatcgtgt cgatgttggt gcccgggggc tgctggatga
     5521 agccgtcgat ggcgtcgcgc ttcaccggcg gcaggtagtc gagcggcggc agcagcgccc
     5581 atgtgaggac cacgggcgcc aggaacagca ccgctacggt gatgtagcgg cgcttcgcgg
     5641 aatctgtcca gcgcacgatc cagccggcga tggcggcata cctgtcgttg gaacccttgt
     5701 gcacgttttc ggtgttgagc cagcgcgatg ccgccagcgg cagcgccgta accgcgacga
     5761 tcagcgaggc aatcaccgcg atggagatag tgagcgccag gtcggcgaag agctgcgaca
     5821 cggcgtcgcg catgaacacg atcggaagga agactattac cgcggtgacg gtggaagcga
     5881 gcaaggctgg ccacacctgc tttgcgcccg cgagcgcggc ctccatgcgt gccagcccgc
     5941 gttcgcgctg ctgcgcaatg gcttcgagca tgacgatggc tgtgtcgagc gcctggccga
     6001 cgccgaaggc gagacccgcg agcgagatca cattgagcgt gcgcccgaag gcgtagagca
     6061 tcaccagcgt gacagtgagg cagatcggga tcgacaaacc gactaacaag gtggcgcgcc
     6121 ggtcgcgcac gaagtaccac aacaccagga ccgccagcac gatgccggcg aacaggttgc
     6181 ccgtgaccat gtcgatggcc tggccgatga acaccgaagg atcgaacgac tgcgcaatcg
     6241 acaggcccat cgtcttgagc tcgttgtcgc gcagcgtaaa gatttccgcc ttgaccgcgt
     6301 tcagcgtctc gagaacattg gcgtcgttct ctttcatgat ctggatgccg atcgccgggt
     6361 tgccgttctg cagcgcgaga tcggtgcgat cgccgcgctt cacctggatg gtggcgatgt
     6421 ccccgagccg gatcgggcgg ccgtcgcgcc actcgagcac cagctcggaa aactggtcca
     6481 tcgaatagcg gcccgcgaag cgcatggtgt actggcggcg gccgatgtcg acgaagccac
     6541 cggagacgtc atcggcactg cccgccacct gcgcgatgcg cgggatctgg ataccgagct
     6601 cggcggcgcg cgccgggtcg aacacgatct gcagctcctc gtcggagccg aagttgatgc
     6661 gtacgttgga gacgcccgga atcgactcga tgcggctgcg gaacaactct tcgatcttgc
     6721 ggcggtagtt gtcgatgggg ccgggcgttc cgggcagcag ctgcacgaag aaccaggaca
     6781 gcgtgttgtt cgggccattg ccgccgtcct cgcccagcga tatctgcggt gcctgcgcgt
     6841 cgcgcggcag cggcggcagc tggttcatgc ggctgatcac ctccagcatc gtcgcctgca
     6901 tctccgtgcc cacggcgaac tgcaggttga tgaacgaact gcccgcgttg gcgaaggcat
     6961 tgagcgaacg caaaccgcgg atgccctgaa gcgcgcgctc ctgaggttcg acgatctcgg
     7021 attcgatctc cgtgggcgcc gcggcgcgcc actgcgtgaa gatcgagatc accggttcct
     7081 cgatgttcgg gaacagctgg atgggtagtt tgaccagcgc gaagatgccg agcagcgcga
     7141 ccatcaggat ggccaccagc acgcccgccg gattacgaag cgaggtctcg gtgagattca
     7201 tgaacgtgct gcactgatgc gccaaggaat gacgccacga taagggcttg gatgcgccgg
     7261 aagcgccgtg gtttacacgg cttggcgcgg gcttaggtca ctctggcgtc aatgccgcgc
     7321 tgccaggaat tatggttggt gccgggtggg gaaa
//
LOCUS       DASQJS010000239         4062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4218573, whole genome shotgun sequence.
ACCESSION   DASQJS010000239 DASQJS010000000
VERSION     DASQJS010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4218573"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..537)
                     /locus_tag="VEW08_09695"
     CDS             complement(169..537)
                     /locus_tag="VEW08_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41053.1"
                     /translation="MQAENVKPPSPTYGLRPGALLVSGMINLLGALLVILAMPGAVSD
                     NRLASAWALMQFLAGVWAGVLGANSPFLHGLVAGLPALALGLVLSSPLPAQFVIVAWA
                     LVPAAALLAAALMRFMRRRN"
     gene            757..2892
                     /locus_tag="VEW08_09700"
     CDS             757..2892
                     /locus_tag="VEW08_09700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYJ41054.1"
                     /translation="MKHFVSRCVRRPMVCLFACLALVIPMIAATAPKRVPTHEDIWLM
                     KRVGAPQVSPDGRWLVVSVVEPAYDDNAQLSDLWLIDTAARHLSRRLTSTRRPESGVI
                     WNPDSTRIVFTAQRDSDELPQIYSLDLRAGGEAQRLTSLSGGARGPVFSHDGRQLAFV
                     SLMYPQAADDTANKALVEKRHARKSNARIYDGFPIRSWDRWLDDRQARIFVLSLDEDG
                     LPVGSPRDLLAGTKLAASAGFAGRQTDTGEEIEIEFTPDNQSVVFAATTNRNAAAYAF
                     TDSQLFVASVKGGEPKQLTQGNNTWSQPRFTPDGRILLAMLEVQGSSVYNASRLAAFT
                     WPDTSKHHIVTDGIDRSVSTFTVSPDSRTVYFTAEDGGNEKLYSTRVGGGTVQTLFGV
                     EKGSYTNLTMPARADKLALFANWESASSPGEIALIAPQNGRALVLTKFNNARASQLDL
                     PPIESLWFTSSRGKRIHSFVVRPPGFDASKKYPLFVVMHGGPHGMWRDQFFVRWNYHL
                     LATPGYVLLLTNYSGSTGFGEEFARSIQGDPLKGPAEEINEAADAAIKQFSFIDGSRQ
                     CAGGASYGGHLSNWLQGSTTRYKCLVSHAGLVNLEAQWGTSDTIYSREVNLGGPPWEQ
                     AAVWREQNPIRYAAKFRTPVLVTVGENDFRVPLNNTLEYWSALQRMQVPSRLIVFPDE
                     NHWIQKGEDSRLFYAEIAAWLERWLKPAD"
     gene            2859..3692
                     /locus_tag="VEW08_09705"
     CDS             2859..3692
                     /locus_tag="VEW08_09705"
                     /inference="COORDINATES: protein motif:HMM:NF016973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF695 domain-containing protein"
                     /protein_id="HYJ41055.1"
                     /translation="MAGTLAQARGLNREYTRSEPTLSDEWDFYFARVNDAVSSIFVDL
                     GIRDDVPVETRPWLLWVWVQLLAPKPDGLATNEESRRLHEIGESLDSLVSATCGAQLV
                     GRLTGNGRREFYFYAAEPGELTTSVASALKAFPDYKYETGSTFQPEWEQYLTLYPSET
                     NLERMHNRRLLEALAKQGDAHEVPRKVDHWLHFADEASRDACRETLLAIEFAVEDEGM
                     SEEEGEDMPFTLVMSRVDSVDTHTINGITLELARLAGEYCGRYDGWESPVIAAAPTVN
                     S"
     gene            complement(3848..>4062)
                     /locus_tag="VEW08_09710"
     CDS             complement(3848..>4062)
                     /locus_tag="VEW08_09710"
                     /inference="COORDINATES: protein motif:HMM:NF017855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HYJ41056.1"
                     /translation="LLVLYGPYLENGHAVQSNLDFDASLKRRNAEWGLRELEEVTRVA
                     AGHSLQRLQIVRMPANNLTVVFGRSL"
BASE COUNT          787 a         1310 c         1278 g          687 t
ORIGIN      
        1 cactacttca gcacctacaa gatggtgccg ggcgaaccta acaagatgca gatctcgggc
       61 aaatacggct tcgaccacgc gaaggcggtc atcgaagcgg cgagcgcgga ttacgacgag
      121 aaattcggtg aggccagcga cgaacggcgc cgcagctaga ccccattctc agttgcgcct
      181 tctcatgaag cgcatgagcg ccgcggcgag cagcgctgcc gccggcacga gcgcccaggc
      241 cacgatcacg aactgcgcgg gcaacgggct cgaaagcacg agtcccagcg ccagcgcggg
      301 cagtcccgcc accaggccat gaaggaacgg cgagttggcg ccgagcacgc cggcccatac
      361 gcccgccagg aactgcatca gcgcccaggc cgaagccagg cggttgtcgc tgactgcgcc
      421 cggcatcgcc aggatgacga gcagtgcgcc cagcaggttt atcatcccgc tgaccagcag
      481 cgcgcccggg cgcaggccat aggtcggcga gggcggcttc acgttttcgg cttgcaaatg
      541 agtaccttct tcctgtccct gttgagcaac gatagatagg cgagcccttg ctcgcgcgat
      601 cccgatgtct tcgctgccga cgccaagggc gatcgcgccg ccgaacctcg gcacgtacga
      661 tgctgaatat ggcaagcgcg agcgatagtc tcgcgtccgc gccagggtga ggcaagtggt
      721 gcaccatcgg accgcggttt gcgataacgt gggcacatga aacatttcgt atcccggtgc
      781 gtgcgccggc ctatggtgtg tttgtttgcc tgtctggcgc tcgtgatccc gatgatcgcc
      841 gccacggcgc ccaaacgcgt tcccacgcac gaagacatct ggctcatgaa gcgcgtcggc
      901 gcaccccagg tgagccccga cggtcgatgg ctcgtggtat cggtcgtcga gcccgcatac
      961 gatgacaatg cgcagctcag cgacctgtgg ctgatcgaca ccgccgcccg gcatttgagt
     1021 cgccggctca cgtctacgcg ccgcccggag agcggcgtca tctggaatcc cgacagcaca
     1081 cgcatcgtgt tcaccgcgca gcgcgacagc gacgagctgc cgcagattta ttcactcgac
     1141 cttcgcgccg gcggcgaagc gcagcgcctg accagcctgt cgggtggcgc gcgggggccg
     1201 gttttctcgc atgacggccg gcagctggcg ttcgtctcgc tcatgtatcc gcaggccgcg
     1261 gatgacacgg ctaacaaggc gctcgtcgaa aagcgccacg cccgcaaatc caacgcgcgt
     1321 atctacgacg gttttcccat acgcagctgg gaccgatggc tggatgatcg tcaggcgcgc
     1381 atttttgtac tgagtctcga cgaggacgga ttgcccgtcg gaagtccgcg cgatcttctg
     1441 gccggcacga agctggcggc cagcgcggga tttgccgggc ggcagacgga taccggcgaa
     1501 gaaatcgaaa tcgaattcac gcctgacaac cagtccgtcg tcttcgcggc cacgaccaat
     1561 cgcaatgcag cggcctacgc cttcaccgat tcacagctat ttgtcgcgag cgtcaagggt
     1621 ggcgaaccga agcaattgac ccagggcaat aacacctggt cgcagccccg cttcacgccc
     1681 gacggccgca tcctgctcgc catgctcgaa gtccagggct cgagcgtcta caacgcgtcc
     1741 cggctggcgg cattcacgtg gcccgatacc agcaagcacc acatcgtcac tgacggcatc
     1801 gatcgttcgg tgagcacgtt cacggtgtcc ccggactcgc gcaccgtgta cttcacggcc
     1861 gaagacggcg gcaacgagaa gctgtactcc acgcgcgtgg gtggcggcac ggtgcagacg
     1921 ctgttcggtg tggaaaaagg cagttacacc aacctgacca tgccggcccg cgccgacaaa
     1981 ctcgcgctgt tcgcgaactg ggaaagcgcc agctcgcccg gcgaaattgc gctgatcgcg
     2041 ccgcagaacg gcagggcgct ggtgctcacg aagttcaaca acgcccgtgc ctcgcagctc
     2101 gacctgccgc ccatcgaatc cctctggttc accagcagcc gtggcaaacg tatccacagc
     2161 ttcgtcgtgc gtccgccggg attcgatgcg tcgaagaagt atccgttgtt cgtggtgatg
     2221 cacggcgggc cgcacggcat gtggcgcgac cagttcttcg tgcgctggaa ctatcacctg
     2281 ctggcgacgc ccggttacgt gctgctgctc actaactact ccgggtcgac cggcttcggc
     2341 gaggaattcg cgcgttccat ccagggcgat ccgctcaagg gtccggccga agagatcaac
     2401 gaagcggcgg acgcggcgat caagcagttt tcgttcatcg acggcagtcg ccagtgcgcc
     2461 ggcggcgcga gctacggcgg tcatctgtca aactggctgc agggctccac gactcgctac
     2521 aagtgtctgg tgagtcacgc cgggctcgtg aatctcgaag cacaatgggg taccagcgat
     2581 acgatctact cgcgcgaagt gaacctgggc ggcccgccct gggagcaggc cgcggtgtgg
     2641 cgcgagcaga acccgatccg ttacgcggcc aagttccgca cgccggtgct ggtgacggtg
     2701 ggcgaaaacg attttcgggt gccgctgaac aacacgctcg aatactggag cgcgctgcag
     2761 cgcatgcagg tgccgagccg cctcatcgtc ttccccgatg agaaccactg gatacaaaag
     2821 ggcgaagaca gccggctttt ttatgcggaa atcgccgcat ggctggaacg ctggctcaag
     2881 cccgcggact gaatcgcgaa tatacgagga gcgagccgac cttgtccgac gagtgggatt
     2941 tctatttcgc gcgcgtcaac gacgcggtgt cgtccatttt cgtcgacctc ggaatccgcg
     3001 acgacgtgcc ggtcgagacg cggccgtggc tgctgtgggt atgggtgcaa ttgctggcac
     3061 cgaagcccga tgggctcgcg accaacgagg aatcgcgccg gctgcacgag atcggtgaat
     3121 cgctcgattc gctggtgtcg gccacctgcg gagcgcagct cgtcggccgc ctcaccggca
     3181 acgggcgccg cgagttctac ttctatgcgg cggagcccgg ggagctcacc acctccgttg
     3241 cgagcgcgct gaaggcattc ccggattaca aatatgaaac cggcagcacg ttccagcccg
     3301 agtgggagca gtacctcacc ctgtacccat cggaaaccaa tctcgaacgc atgcacaacc
     3361 ggcgcctgct cgaagcgctg gcgaagcagg gtgacgcgca cgaagtacca cgcaaggtcg
     3421 atcactggct gcacttcgct gacgaagctt cgcgcgacgc gtgtcgcgag accttgctgg
     3481 ccatcgaatt cgcggtggaa gacgagggca tgtcggaaga agaaggcgag gacatgccgt
     3541 tcacgctggt gatgtcgcga gttgacagcg tggataccca caccatcaac ggcatcacgc
     3601 tggagctcgc gcggctcgcg ggcgaatact gcggtcgcta cgacggctgg gaatctccgg
     3661 tcattgcggc cgccccgacc gtcaatagtt agccatccgc tggcgtcctg cctggatgta
     3721 tggacgcatc atgaccggca gaacggcctc gaacgctggc ggcgagccgg ccgcaggcga
     3781 attttcatct gatacccggt agccacaaca gttggcccca tcaacgcgcg gtgcgaatcc
     3841 gcaatcccta gagcgacctg ccgaacacca cggtcaggtt gttagccggc attcgcacga
     3901 tctgcaggcg ctggaggcta tggccagccg ccacgcgcgt gacttcttcc agttcccgca
     3961 gaccccactc ggcattgcgc cgcttgagtg aggcgtcgaa gtccaggttg gactgcaccg
     4021 catggccgtt ttccagatac ggtccataga gaacgagcaa tc
//
LOCUS       DASQJS010000240        11215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1080885, whole genome shotgun sequence.
ACCESSION   DASQJS010000240 DASQJS010000000
VERSION     DASQJS010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11215
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080885"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VEW08_09715"
     CDS             complement(<1..781)
                     /locus_tag="VEW08_09715"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HYJ41057.1"
                     /translation="MGAGETGIREGSLQAPSRHPLDWRSADFVDEAALEKELERVFDV
                     CHGCRRCFSLCNSFPTLFDAIDESETLELDGVDKKVYWEVVDHCYLCDMCFMTKCPYV
                     PPHPWNIDFPHLMLRAKHVQHRKGEHSFRDKLLSNTRGLGSIAGIPVVAEIVNAVNGT
                     KVGRKLLEATLGVDATAPIPKFHSNTGTKRARREAGKATQVTPTADTRGKVALFVTCY
                     GNRNEPHLPEDLTVVFEHNGIPVKVVGKEKCCGMPKLELGDL"
     gene            complement(794..1207)
                     /locus_tag="VEW08_09720"
     CDS             complement(794..1207)
                     /locus_tag="VEW08_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HYJ41058.1"
                     /translation="MNLKGSKTEKNLKDAFAGESQANRRYLYFAAKADVEGYNDVSTV
                     FRSTAEGETGHAHGHLEYLESVGDPATGLPIGETKMNLKAAIAGETHEYSDMYPGMAK
                     QAREEGFSEIADWFETLAKAERSHANRFQKALDAI"
     gene            1363..1644
                     /locus_tag="VEW08_09725"
     CDS             1363..1644
                     /locus_tag="VEW08_09725"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HYJ41059.1"
                     /translation="MKSKPQRDNKLPDFEHSLTELEALVAKLEQGDVPLEEALKTFER
                     GVALTRQCQTALRTAQQKVEVLLARNGDESIEPFAVDDDEDDADDTAGR"
     gene            1625..2554
                     /locus_tag="VEW08_09730"
     CDS             1625..2554
                     /locus_tag="VEW08_09730"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HYJ41060.1"
                     /translation="MTPPVADAARPVAPLFLADRVPLYQERVEKVLARALEIEGGETS
                     RLIEAMKYSTLAGGKRVRPVLVYATGEALGAPLELLDAAAAAVELIHVYSLVHDDLPA
                     MDNDDLRRGRPTCHRAFDESTAILVGDALQARAFEVLANAPAAINANARLEMLRVLAD
                     AIGTRGMAGGQAIDLESVKQSLGEAALERMHRQKTGALIQASVLLGAISAGLQDVPQR
                     AALAEFGAEIGLAFQIQDDILDVEGTTTTLGKRAGADADRVKPTYPSVLGLEKSREQA
                     FARRDRAIAALSPWGTRFAYLSEFAKFLVARAN"
     gene            2605..4503
                     /gene="dxs"
                     /locus_tag="VEW08_09735"
     CDS             2605..4503
                     /gene="dxs"
                     /locus_tag="VEW08_09735"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HYJ41061.1"
                     /translation="MSNVPEPFALLRSLNSPADLRAQPENRLGEVCDELRDYLIKTVA
                     TRGGHFAAGLGTVELTVALHYVFETPRDRLVWDVGHQAYPHKVLTGRRDRLDTIKQNG
                     GLAPFPSRAESEYDTFGVGHSSTSISAALGMAVAAELARDDRKVVAIIGDGAMTAGMA
                     WEALFNAGARKTNLLVVLNDNDMSISENVGALSNYFAKVLSGRMYSTLRSGGKKILRQ
                     MPTMWELARRSEEMMKGMVLPGTLFEEFGFNFIGPINGHDIKALVTNLRNMRHLEGPQ
                     LLHVVTRKGKGYDPAEKEPIKWHGPGPFDPVSGTIFKEKATGPTYSQVFGQWLCDMAS
                     LDPRIVAITPAMREGSGLVEYSKKFPQRYFDVAIAEQHAVTFAAGLACEGWKPVVAIY
                     STFLQRAYDQLIHDVALQNLPVVFALDRAGLVGGDGATHQGSYDYSFLRCIPNLTVMA
                     PADENECRQMLYTATTLPGPVAVRYPRGQGPGVAPVAEMTALPVGRAELRREGKSGLL
                     ILAFGALVRSAEIIGDRLDATVVNMRFVKPLDEELVLRLAAKHNALVTIEENAVAGGA
                     GSAVIELLASCATLLPTLTLGIPDCFVEHGSRDECLVKAELDQASLAAAVERWWSPLA
                     GKQARSGT"
     gene            4610..5437
                     /gene="folE2"
                     /locus_tag="VEW08_09740"
     CDS             4610..5437
                     /gene="folE2"
                     /locus_tag="VEW08_09740"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HYJ41062.1"
                     /translation="MNPVAAPQARVVAVEDVQSRADTRQIPINQVGIKDINHPVKVKD
                     RSAGEQHTVANFSMYVNLPHNFKGTHMSRFVEILHREREISVESFGSLLAEMTERLEA
                     ESGHIEMSFPYFVMKKAPVSGVESYMDYHATLTGKRVKGENQMWVRVVVPVTSLCPCS
                     KKISERGAHNQRSHVTIAARLREHMWIEELIEIAESEASCELYGILKRPDEKYVTERA
                     YDNPKFVEDMVRDVAVRLNDEPRVGAYVVESENFESIHNHSAYAQIERDKDTDSSGA"
     gene            5558..6223
                     /locus_tag="VEW08_09745"
     CDS             5558..6223
                     /locus_tag="VEW08_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41063.1"
                     /translation="MSIPLPTFRIRCASAAIAALLACTSLVQAGPLVVSAWQHDGKAL
                     AGAVVTVEADSPAHPPSPPAQATMDQLNLAFVPDVLVVPEHSTVQFPNSDVIGHQVYS
                     FSSARRFQLPLYRGKPYPPVTFDEAGIVTLGCNIHDNMLAYIVVTRAPWFGRTDASGK
                     WAVPNLPAGRFRLRLWHPLLNEPLEVEHLFDAGAAEERVEIRLSRNLRPAPLTGRPHS
                     WDY"
     gene            6211..7437
                     /locus_tag="VEW08_09750"
     CDS             6211..7437
                     /locus_tag="VEW08_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41064.1"
                     /translation="MGLLTHKRAIWLCACLVGTQAQAGDWELALDLRAVSSDGRESFL
                     DNGQGKLRFDEDHQGIQLGRLRAAWNQPLGEVFSAHVEASTWDDDDKNPIDLTEAYIE
                     YRPYPRAGVRSRLRLGAFYPPMSLESRAVGWETPYTITPSAISSWIGEEIRTVGLEGQ
                     IDWLGTRLGHSFDLQLTAAVFGWNDPAGTMLAAHGFALHDRQTTLFGRVGAPQNDPAF
                     AKKELFHEIDDRPGYYLGAQARYLDRVVLNLLHYDNRADPTVEMPSIRDFAWLTKFDA
                     AALRIETGNGWTAILQALDGSTYIAPGSFWLDWEFASQSALLAKRVGVHMVALRYDTF
                     KVEFMDDPTESGSEDGHAWALAYTFDPGKQWRFALEWLQVESDVPARVEYLGEPAFAR
                     ESKLEFSARYLLSGSF"
     gene            7580..8368
                     /gene="modA"
                     /locus_tag="VEW08_09755"
     CDS             7580..8368
                     /gene="modA"
                     /locus_tag="VEW08_09755"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF007958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYJ41065.1"
                     /translation="MIIFSRPAKFLAACVAAMAFVGAAQAEEPKKPDLLVFAAASLTD
                     VLGELAPAWEKSSGVTVKLSFAASSVLARQIEAGSNADVFISADQEWMDYLQTRNLIS
                     KPSRVDLVGNRLVLIAPADSKIELEIRHGFDLAGALAGGRLSTGDPDTVPVGRYARAA
                     LTSLGVWHQAADRLVRADNVRSAMTFVSRGEAPLGIVYATDAQIDKKVRVVATFPDNS
                     HDPITYPAAATVSAGPGAAGFLAFLRSKDAAPSWKKFGFLELAK"
     gene            8365..9954
                     /locus_tag="VEW08_09760"
     CDS             8365..9954
                     /locus_tag="VEW08_09760"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HYJ41066.1"
                     /translation="MTHTLRAAVALALGLAVSPAFADEAALKAEVDALRAAISEQRAL
                     LDSQARLLEAQQAQLEALSRQMGQPKVAAAETQKVAAPESPKLTFNNNRPTITSADGK
                     SSFAVRANVQLDGAMYGEDKEGPLATDYRRGSVGATANRENNAARDFSDGFYFRRARF
                     GVEGTIASAFNYRLLLELGGSGTEGPTRINDAWIAYTGLAPFTFQLGAFSPPANMDDG
                     TSPEDLAFLERASVSELSRSLGGADGRIGLGLKANGKRWMSAFTITTRTVNEAEVFDG
                     QLAAVGRAGGLIATGSDYNVHLGASGTYVFSPADLGSSATPPRHAIRFRDRPEIRVDS
                     VRLIDTGAIDAAHASVLGMEFGANWKSFYLQGEHFWFDVERRGAAALPDPDFAGYYVQ
                     GSWMLTGESRRYNPVTGSFQNPRPKEIFSREGGAGAWELAARYSNMNLNFQEGAEGVA
                     TVPGSVRGGEQNVLTLGVNWYLNPNFKVMMNYLLIDVDRLNPAGPGNTAPFGASPATP
                     PIGVEIGQDLNVIALRTQYSF"
     gene            complement(10044..>11215)
                     /locus_tag="VEW08_09765"
     CDS             complement(10044..>11215)
                     /locus_tag="VEW08_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYJ41067.1"
                     /translation="DVAIKVYNMDQDPADERAKIARKMFLSEAHMVGMLQHPHILPIY
                     DAGEENGRCYIVTEHVHGARTLSSYCRPDNLLRVDDVVEIMFKCAKALHYAHTRGVIH
                     RDIKPSNIMLTQDSDVRIIDFGIALVADSEISRIEGIAGSPSYMSPEQVQSLELTHHS
                     DLYSLGAVMYELLTGIRPFRAGNLVKLLHQIVYATPPPIHTQRKDIPEELEEIVAQAM
                     LKTPDKRQKSGADFAAELTRVHQRLREQNARIDQQEHFDVLRRLRFFHEFSHAEIWEV
                     LRASTMQDYPAGDEIVKEGEMDDRFYILVNGKCLVERHGKTLGSIDSGDCFGETSYVR
                     GARRTATIKSESAVTVLKVSSTLLEQVSAACQLRFNRVFLRALIGRLQGNDQPLE"
BASE COUNT         2057 a         3658 c         3458 g         2042 t
ORIGIN      
        1 cgagatcacc aagctcgagc ttgggcatgc cacagcattt ttccttgccc acgaccttca
       61 cgggaattcc gttgtgctcg aacacgaccg tgaggtcttc gggcaggtgc ggctcgttac
      121 ggttgccgta gcaggtgacg aacagcgcca ctttgccgcg cgtatccgcg gtcggcgtga
      181 cctgcgtcgc cttgcccgcc tcgcgtcgcg cacgtttcgt gccggtgttg gaatggaatt
      241 tcgggatcgg cgcggtggcg tccacaccga gcgtggcttc taacaacttg cggcccacct
      301 tcgtgccgtt caccgcgttg acgatctcgg caaccacggg aatgccggcg atgctgccga
      361 gcccgcgggt gttcgacagc agtttgtcgc ggaaggagtg ctcgcccttg cgatgctgca
      421 cgtgcttggc gcgcagcatc aggtgcggga agtcgatgtt ccagggatgc gggggcacat
      481 agggacactt cgtcatgaag cacatgtcac acaggtaaca gtgatcgacg acctcccagt
      541 aaaccttctt gtcgacgcca tcgagctcga gcgtttcact ctcgtcgatc gcatcaaaga
      601 gggtcggaaa tgaattgcac aggctgaagc agcggcgaca gccatggcac acgtcgaaga
      661 cgcgctcgag ttctttctcg agcgcggcct cgtcgacgaa atcagcactc cgccagtcga
      721 ggggatgacg cgagggagcc tgcagactgc cctcgcgtat tccggtttct cccgcgccca
      781 cgcagcgtgg ccgtcagatc gcgtccaggg ctttctggaa gcggttcgcg tgcgaccttt
      841 cggccttggc aagggtttcg aaccagtcgg cgatttcgga aaaaccttcc tcgcgcgcct
      901 gctttgccat gcccggatac atgtcgctgt actcgtgcgt ttcgccggcg atggcggcct
      961 tcagattcat cttcgtctcg ccgatcggca ggcccgtggc cggatcgccc acggactcca
     1021 ggtactcgag gtgaccatgc gcatgacccg tctcgccttc ggcggtggaa cggaacacgg
     1081 tcgacacgtc gttgtaaccc tcgacgtcgg ccttggctgc gaaatacagg taacggcgat
     1141 tcgcctgcga ttcgcctgcg aatgcatcct tcagattctt ttcggtcttg ctgcctttga
     1201 ggttcacggc tcgctcctcc atcaattgat aagaatggct ctcatttaga cttgttctaa
     1261 gagtggcgga ctctacggcc gccgccttgc gggcgtcaat cggggccgtc atttgcatgc
     1321 gtgggtttga atcgatgtct cctaccgttt aacgtcgcgc gcgtgaaatc aaaaccccaa
     1381 agagataaca agctaccgga cttcgaacat tcgctgaccg aactcgaagc cctcgtggcg
     1441 aagctcgaac aaggcgacgt gccactcgaa gaggctctca agactttcga gcgcggtgtt
     1501 gcgctcaccc gccagtgcca gaccgcgttg cgcacggcgc agcagaaagt ggaagtgctg
     1561 ctggcccgca acggcgatga aagcatcgaa ccgttcgcgg tcgatgatga cgaagacgac
     1621 gccgatgaca ccgccggtcg ctgacgctgc gcgaccggtg gcgccgttgt tcctggccga
     1681 tcgtgttccg ctctaccagg agcgcgtcga gaaggttctc gcgcgtgcgc tggaaatcga
     1741 aggcggcgaa acgtcccggc tcatcgaggc catgaaatac agcacgctcg cgggcggcaa
     1801 gcgcgtgcgg ccggtgctgg tatacgccac tggcgaagcg ctcggcgcgc cgctggaatt
     1861 gctggatgcc gcagcggctg cggtcgaact cattcacgtc tacagcctgg tccacgatga
     1921 cctgcccgcc atggacaacg acgaccttcg ccgcggccgt ccgacctgcc atcgtgcctt
     1981 cgacgaatcc actgccatcc tcgtcggcga tgcgctgcag gcgcgggcat tcgaggttct
     2041 cgcgaacgcg ccggccgcga tcaatgcaaa cgcgcgtctc gaaatgctgc gcgtcctcgc
     2101 cgatgccatc ggtacgcgcg gcatggccgg cggacaggcg atcgatctcg agtctgtaaa
     2161 acaatctttg ggtgaagcgg cgctcgaacg catgcaccgg cagaagaccg gcgcgctgat
     2221 tcaggcgagc gtactgctcg gcgctatttc agccgggctc caggacgtac cgcaacgcgc
     2281 ggcgctcgcg gaattcggcg cggagatcgg cctggccttt cagatccagg acgacattct
     2341 cgatgtcgaa ggcaccacca ccacgctcgg caagcgcgcg ggcgcggatg ctgatcgcgt
     2401 gaaaccgacg tacccctccg tcctgggcct ggaaaaatcg cgcgaacagg cattcgcgcg
     2461 ccgtgatcgc gcgattgctg cactcagccc ctggggcacg cgcttcgcgt acttgagcga
     2521 attcgcaaaa ttcctcgtgg cgcgcgccaa ctgaatgttt tggcctgcct gtctccccgg
     2581 acaggcgtgt aaacttcact cgcaatgtcc aacgtcccgg aaccattcgc gctgttgcgc
     2641 tcgctcaatt caccggcgga cctgcgcgca cagcccgaaa accggctggg cgaagtctgc
     2701 gacgagctgc gcgactacct gatcaagacc gtcgccacgc gcggcggaca tttcgccgcg
     2761 gggctcggca cggtcgaact caccgtcgcg ctgcattacg ttttcgaaac accgcgtgac
     2821 cggctggtgt gggatgtcgg ccaccaggcc tatccgcaca aggtgctcac cggccggcgc
     2881 gaccggctcg acaccatcaa gcagaacggc ggtcttgccc ctttcccctc gcgcgcagaa
     2941 agcgaatacg acacgttcgg cgtcggtcac tcgagcacct cgatcagcgc cgcactcggc
     3001 atggcggtag ccgcggagct cgccagggat gaccgcaagg tggtcgccat catcggcgac
     3061 ggcgcaatga ccgccggcat ggcctgggaa gcattgttca acgccggcgc acgcaagacc
     3121 aacctgctgg tcgtgctcaa cgacaacgac atgtcgatct ccgagaatgt cggcgcgctg
     3181 tccaactact tcgccaaggt actgtcgggc cgcatgtatt ccacgctgcg gtcggggggc
     3241 aagaagattc tcaggcagat gcccaccatg tgggagctcg cgcgccggtc cgaagagatg
     3301 atgaagggca tggtcctgcc cggaacgctg ttcgaggagt tcggtttcaa tttcatcggg
     3361 cccatcaacg gccacgacat caaggcgctg gtcacgaacc tgcgcaacat gcggcacctc
     3421 gaagggccgc agctgctgca cgtggtcacc aggaagggca agggctatga cccggccgag
     3481 aaagaaccga tcaagtggca cggcccgggt ccgttcgatc cggtcagcgg caccatcttc
     3541 aaggaaaaag ccaccggacc cacctactcg caggtgttcg gccagtggtt gtgtgacatg
     3601 gcgagcctcg atccgcgcat agtcgccatc actccggcca tgcgcgaagg ctcgggcctg
     3661 gtcgaatact cgaagaaatt tccgcagcgt tatttcgacg tggccatcgc cgaacaacat
     3721 gccgtgacgt tcgctgccgg tctcgcctgc gagggctgga agccggtggt cgctatctac
     3781 tccacgttcc tgcagcgcgc ctacgatcag ctgattcacg acgtggcgct gcagaacctg
     3841 cccgtggttt tcgcgctgga tcgcgccggg ctcgtcggcg gcgacggggc cacgcaccag
     3901 gggtcgtacg attactcatt cctgcgttgc attcccaacc tcaccgtcat ggcgcccgcc
     3961 gacgagaacg aatgccggca gatgctgtac accgcgacca ccctcccggg gccggtggcg
     4021 gttcgatatc cacgcgggca aggtccgggc gtcgctccgg tggcggagat gactgcattg
     4081 ccggtcggtc gcgcggaact ccgccgcgaa ggcaaaagcg gccttctgat cctcgccttc
     4141 ggcgcgctgg tacgttcggc ggaaatcatc ggcgaccgtc tcgatgccac ggtcgtcaac
     4201 atgcggttcg tcaaaccact cgacgaagag ctggtgctgc ggctcgcggc gaaacataac
     4261 gcgctcgtga ccatcgaaga aaacgccgta gccggtggcg ccggctctgc ggtcatcgag
     4321 cttctcgcca gctgcgccac cctgctgccc acgctcacgc tcggcattcc cgactgcttc
     4381 gtcgagcatg gttcgcgcga cgagtgcctc gtcaaggccg aactggatca ggccagtctc
     4441 gcggctgcgg ttgagcgctg gtggagcccg ctcgccggca agcaggcgcg ctccggcacc
     4501 tgaaacaggc caccgcaggc gcaattgcgg tgcattaccc ccattgaggg gttgatccaa
     4561 cgccgaaagc ccgcaaactg tgcgggcttt tattttttca ggtccttaca tgaaccctgt
     4621 tgctgccccc caggcccgcg tcgtcgctgt cgaagacgtg cagagccgcg ccgacactcg
     4681 ccagatcccg atcaaccagg tcggtatcaa agacatcaat cacccggtga aggtgaaaga
     4741 ccgctccgcc ggcgagcagc acaccgtggc caacttcagc atgtacgtga atctgccgca
     4801 caacttcaaa ggcacgcaca tgtcgcgctt cgtggagatc ctgcaccgcg aacgcgagat
     4861 ttccgtcgag tccttcggga gcctgctggc ggagatgacc gagcgcctcg aggcggagtc
     4921 cgggcacatc gagatgtcgt tcccctactt cgtgatgaag aaagcgccgg tgtccggcgt
     4981 cgagagctac atggattacc acgccactct caccggcaaa cgcgtcaagg gcgaaaacca
     5041 gatgtgggtc cgcgtggtcg tgccggtcac gagcctgtgc ccgtgttcga agaagatctc
     5101 cgagcgcggc gcgcacaacc agcgttctca cgtcaccatc gccgcccgcc tgcgcgagca
     5161 catgtggatc gaagagctca tcgagatcgc ggaaagtgaa gcgtcgtgcg agctgtacgg
     5221 aatcctcaag cgacccgacg agaaatacgt gaccgagcgt gcctatgaca atccgaagtt
     5281 cgtggaagac atggttcgtg acgtcgccgt gcgactgaac gacgaaccgc gggtcggcgc
     5341 ctacgtggtc gagtccgaga atttcgagtc catccacaac cactccgcat acgcgcagat
     5401 cgaacgcgac aaggacaccg attccagcgg tgcctagctg agaagcgcaa gcccgatgcg
     5461 acgggcgtca cagtttggcg cctgcgcgca aatcacccgc gacgtcacga gaagtcggtt
     5521 ggagtaccgg cgctccgcat cggatcgaat gcgtgaaatg tcgatcccgc ttcccacgtt
     5581 ccggattcgc tgcgcgagtg cagccatcgc tgcactgctg gcctgcacgt cgctcgtcca
     5641 ggccggtccg ctggtggttt ccgcatggca gcacgatggc aaggcgctgg ctggcgcggt
     5701 cgtgactgtc gaggcggatt cgccggccca cccgccttcc ccgcccgcgc aggcgacgat
     5761 ggaccagctg aatctcgcct tcgtccccga tgtgctggta gtaccggagc actccaccgt
     5821 gcagttcccg aacagcgatg ttatcggcca ccaggtgtat tcattctcga gcgcgcggcg
     5881 gttccagttg ccgctgtatc gcggcaagcc ctatccaccc gtgacattcg atgaagccgg
     5941 tatcgtcacg ctcggctgca acatccacga caacatgctc gcctacatcg tggtgacgag
     6001 ggcgccctgg ttcggccgca cggatgccag cgggaaatgg gcggtgccga atcttcccgc
     6061 gggccggttt cggctgcgtc tctggcaccc gctgctcaac gagccgctgg aagtcgaaca
     6121 cctcttcgac gccggcgcag ccgaagaacg tgtcgagatt cgcctctcgc gcaaccttcg
     6181 acccgcacca ctgacaggac gtccgcactc atgggactac tgactcacaa acgcgcaatc
     6241 tggctgtgcg cgtgcctggt cggtacgcag gcgcaggccg gagattggga actcgcgctc
     6301 gacctgcggg cggtgagctc cgacggccgc gaatcgttcc tcgacaacgg ccagggcaag
     6361 ttgcgcttcg atgaagatca ccagggcatc cagctcggcc gcctccgcgc cgcatggaac
     6421 cagccactcg gcgaagtgtt ctcggcgcac gtggaggcct cgacctggga cgacgacgac
     6481 aagaatccca tcgacctcac cgaggcctac atcgaatacc ggccctatcc gcgcgccggc
     6541 gtgcgctcgc ggctgcggct cggcgcgttc tatccaccga tgtcgctcga gagccgtgcg
     6601 gtcggctggg aaacgccgta caccatcact ccctcggcaa tcagctcgtg gatcggcgag
     6661 gaaatccgca ccgttggcct cgaaggccag atcgactggt taggcacgcg tctcgggcac
     6721 tcgttcgacc tgcagctcac cgccgccgtg ttcggctgga acgatccggc cggcacgatg
     6781 ctcgcagccc acggctttgc gctgcacgac cgacagacta cgttgttcgg ccgagtcggc
     6841 gcgccgcaga atgatcccgc gttcgcaaag aaagaattgt tccacgagat cgacgatcgt
     6901 ccgggttatt acctcggcgc gcaggcgcgc tacctggatc gcgtcgtgct caacctcctg
     6961 cactacgaca atcgcgccga ccccacggtg gaaatgcctt cgattcgtga ctttgcgtgg
     7021 ctgaccaaat tcgacgccgc ggcactgcgc atcgaaaccg gcaatggctg gaccgcgatt
     7081 ctgcaggcgc tcgatgggtc cacgtacatc gccccgggaa gcttctggct cgactgggag
     7141 ttcgcctcgc agtcggcact gctcgccaaa cgcgtgggtg tgcacatggt ggcgctgcgc
     7201 tacgacacat tcaaagtgga gttcatggac gaccccacgg aatccggcag cgaagacggc
     7261 catgcgtggg cgctcgcgta cacgttcgat cccggcaagc agtggcgctt cgcgctcgaa
     7321 tggctgcaag tcgagagcga cgtgccggcg cgcgtggagt atctgggtga gcccgcattc
     7381 gcgcgcgaat cgaagctgga attctccgcg cgttatctgc tcagcggctc tttctagttt
     7441 tcccgagctt gccgctgacg cggcgctggc ttgcagtttg cgaaaaatcg ccggctcatc
     7501 ggtacactcc actccgtcct gacgcccggc gggatatggc gcgatctgga aaacaacgaa
     7561 atcgggagtg tgcgtcatca tgattatttt cagtcgcccg gcgaagtttc tggcggcgtg
     7621 cgtggccgcg atggcatttg tcggtgccgc gcaagccgaa gagcctaaga aacccgactt
     7681 gctggtcttc gccgcggctt cgctcaccga tgtgctgggt gaactggccc ctgcctggga
     7741 aaagtcttcg ggcgtcaccg tgaagctgtc gttcgctgcg agctccgtgc ttgcgcggca
     7801 gatcgaggcg ggcagcaacg ccgacgtgtt catctccgcc gatcaggaat ggatggacta
     7861 tctgcagaca cgcaacctga tctcgaagcc ctcgcgcgtg gacctcgtgg gcaaccggct
     7921 ggtcctcatc gcacccgccg acagcaagat cgaactcgag atcaggcatg gcttcgatct
     7981 cgccggcgcc ctcgccggcg gccgtctatc cactggcgat cccgacaccg tcccggtggg
     8041 ccggtacgcg cgcgccgcgc tcacttcgtt aggcgtgtgg caccaggcag ccgatcgcct
     8101 ggtgcgcgcc gacaacgttc gcagcgccat gaccttcgtg tcgcgcggcg aggcgccgct
     8161 cggcatcgtt tatgcgactg acgcacaaat cgacaagaaa gtgagggttg tcgctacttt
     8221 ccccgataac agccacgacc cgattaccta tcctgccgcc gccaccgtct cagcggggcc
     8281 gggcgcggcg ggttttctcg ccttcttgcg cagcaaagat gccgcgccct cgtggaaaaa
     8341 atttggattc ctggagctcg cgaaatgaca cacaccctgc gcgccgccgt ggcgcttgcc
     8401 cttggccttg ccgtttcccc ggcattcgcc gatgaagccg ccctgaaggc agaggtggac
     8461 gcactgcgag ccgccatcag cgaacaacga gcgctgctcg attcgcaggc caggctgctc
     8521 gaagcacagc aggcacagct cgaggcgctc tcccgccaga tgggccagcc gaaggttgcg
     8581 gcggccgaaa cgcagaaagt cgccgctccc gagtcgccga aactcacgtt caacaacaac
     8641 cggcccacca ttacctcggc cgacggcaag tcctcgttcg cggttcgcgc caacgtgcaa
     8701 ctcgacggcg ccatgtatgg cgaagacaag gaaggtccgc tcgccacgga ctatcgtcga
     8761 ggctcggtcg gcgccactgc caatcgtgaa aacaacgccg cgcgcgactt cagtgacggg
     8821 ttctacttcc gccgtgcccg tttcggcgtc gaagggacca tcgcgagcgc attcaattat
     8881 cgcctgctgc tcgaactcgg cggcagtggc accgaaggtc cgacgcgcat caatgatgcc
     8941 tggatcgcgt acacgggcct cgcgcctttc acctttcagc tcggtgcatt ctcgccgccc
     9001 gccaacatgg atgacggcac ctcgcccgaa gatctggcgt tcctcgagcg cgccagcgtt
     9061 tccgaactct cacgctcgct gggtggcgcc gatggccgca tcggcctcgg gctcaaggcg
     9121 aatggcaaac gctggatgag cgcctttacg attacgacgc gtaccgtgaa cgaagccgaa
     9181 gtcttcgatg gacagctcgc cgcggtcggg cgcgccggcg gtctcatcgc caccggcagc
     9241 gactacaacg tccatctcgg cgcgtccgga acctacgtgt tcagccccgc cgacctgggc
     9301 tcgtcggcga cgccgccgcg tcacgccatc cgcttccgcg accggcccga aatccgtgtc
     9361 gacagcgtcc gcctcatcga caccggcgcc atcgacgccg cacatgccag cgtgctcggc
     9421 atggagttcg gtgcgaactg gaagagcttc tatctacagg gcgaacactt ctggttcgac
     9481 gtcgaacgcc gcggcgccgc ggccctgccc gaccccgact ttgcgggcta ctacgtgcag
     9541 ggcagctgga tgctcacggg cgagagccgt cgttacaacc cggtcaccgg ctccttccag
     9601 aatccgcgac cgaaggaaat cttctcgcgt gaaggcggcg ctggcgcctg ggaactcgcc
     9661 gcgcgctaca gcaacatgaa cctgaatttt caggaaggcg cggaaggtgt cgcgaccgtg
     9721 ccgggtagcg tgcgcggcgg cgagcagaac gtgctgacgc tcggcgtgaa ctggtatctg
     9781 aacccgaact tcaaggtaat gatgaattac ctgctgatcg acgtggaccg cctgaatccc
     9841 gccggtccag gaaacaccgc tccgttcggc gcctcgcctg ccacgccgcc cattggcgtc
     9901 gaaatcggcc aggacctcaa cgtcatcgcg ctcaggaccc agtacagttt ctaaggcgta
     9961 ctcgttcagg cgtaccgcca caacggaaaa cgccaccctt cggcggcgtt tatgaattgc
    10021 caagagcaat acgctgaagg cacttactcg agtggctggt cgttgccctg gaggcggccg
    10081 atgagcgcac gcaggaacac gcggttgaag cgcagctgac aggccgcgga aacctgctcg
    10141 agcagcgttg agctcacctt gagcaccgtc accgcgcttt cagacttgat cgtcgcggtg
    10201 cggcgcgcac cgcgcacgta actcgtctcg ccgaagcaat cgccgctgtc gatggaaccc
    10261 agcgtcttgc cgtggcgttc caccaggcac ttgccgttga cgagaatgta gaagcggtcg
    10321 tccatctcgc cttccttcac gatctcgtcg cccgccggat agtcctgcat ggtgctggcg
    10381 cgcagcacct cccagatctc ggcgtgtgag aactcgtgga agaagcgcag gcgacgcagc
    10441 acgtcgaaat gctcctgctg atcgatgcgc gcgttctgct cgcgcaggcg ctggtgcacg
    10501 cgcgtgagct cggcggcgaa atccgctccg gatttctgac gcttgtcggg agtcttgagc
    10561 atcgcctggg cgacgatctc ttccagttct tccggaatgt ccttgcgctg cgtgtggatc
    10621 ggcggcggcg tggcatagac gatctggtgc aacagcttca cgaggttgcc ggcgcggaac
    10681 ggccggatgc cggtgagcag ctcgtacatc accgcgccca gcgaatacag gtcagagtgg
    10741 tgcgtgagct cgaggctctg cacctgctcg ggcgacatgt acgacggcga accggcgatg
    10801 ccttcgatgc gcgagatctc ggagtccgcg accagcgcga taccgaagtc gatgatgcgc
    10861 acgtcggaat cctgcgtgag catgatgttc gagggtttga tatcccggtg gataaccccg
    10921 cgcgtatgag cgtagtgcag cgccttcgcg cacttgaaca tgatctcgac cacatcgtcg
    10981 acgcgcagca ggttgtcggg ccggcaatac gaagacagcg tgcgcgcgcc atgcacgtgc
    11041 tcggtaacga tgtagcagcg gccgttctct tcgcccgcgt cgtagatggg caggatgtgc
    11101 ggatgctgca gcatgcccac catgtgcgct tccgacagga acatcttgcg ggcgatcttg
    11161 gcgcgctcgt cggcgggatc ctggtccatg ttgtagacct tgatcgcgac gtcac
//
LOCUS       DASQJS010000241         6715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2967785, whole genome shotgun sequence.
ACCESSION   DASQJS010000241 DASQJS010000000
VERSION     DASQJS010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2967785"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VEW08_09770"
     CDS             <1..961
                     /locus_tag="VEW08_09770"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HYJ41068.1"
                     /translation="YEHDTAGGLMNTDTVSVRADVTVEVVLRYLRMRGDLPDKTDRLF
                     VVDRDDRFLGTLALTRLLTEDPEKPVGDILDPEGSRISPETNAHDVVQLFQDRDLVSA
                     AVVDEHGKLLGRITVDDVVDVIREEAAHEVMSAAGLGDEEDVFAGVIPSTRRRLVWLG
                     INLVTAFMAAAVVSRFEGTIEKVAALAALMPIVASMGGIAGTQTVTLIIRGIALGQVE
                     WVNARWLLWKEIAVGGLNGLIWAAVVGAVTIVWYGTWKIAAIIAAATLINLLAAAAVG
                     VIVPLVLRRLRIDPALSAGVILTTFTDCIGFATLLGLGALFLT"
     gene            complement(932..1693)
                     /locus_tag="VEW08_09775"
     CDS             complement(932..1693)
                     /locus_tag="VEW08_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HYJ41069.1"
                     /translation="MNLGPAEIHVWQSAERPGDEAALEARRGLLEPAELERVDKAYSP
                     KVRLRFLRARVMQRTVLGRYAPDVDPASLRFVKGEHGKPALAPPFESLGLHFNVTHTD
                     GFIAVAVSRLRDVGIDAENLHARTTALKLARRYFTPDEARNLESLPIPEQLARFYALW
                     TLKESWMKATGKGLVAGLDNAEFSLDANHRVVGLSFAAGDVADWRFWQYAPTQEHVLA
                     LALRAPGATQGVAVEKFEWRPGDAPPSGQEQRAQP"
     gene            complement(1697..2803)
                     /locus_tag="VEW08_09780"
     CDS             complement(1697..2803)
                     /locus_tag="VEW08_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYJ41070.1"
                     /translation="MGRAGLILTGGGARAAYQVGVLKALAEMHASPEWPFPIIVGTSA
                     GAVSASILAGNVTRWHQSVGEIEKVWADFHVAQVFRSDAIAMLRAGARWMGSLFTGGM
                     IGPPKSLLDNSPLRELLAKVADFDAIRANVARGDLRALALCATSYFTARSVSFFEAET
                     TISEWSRAQRHGERVPLSLDYLMASLGIPFLFPPMCLHGEYFGDGAMRQTAPLSPAIH
                     LGADRVLVLGVRPTHLDLIPRFVASDSPPTPGQLFGYMLDTLFSDQISADLENLNRIN
                     RLSELAGGVTGTRQIRTMLIAPSVDPSQIAVRHLDSLPVSLRSLMRIIGARGAAGSLL
                     ASYLMFESGYTRELIEMGYRDALGKRDELAAFLA"
     gene            2861..3418
                     /locus_tag="VEW08_09785"
     CDS             2861..3418
                     /locus_tag="VEW08_09785"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYJ41071.1"
                     /translation="MLALRRTLVTALIAVSASVCAQAPAASPAEGTAAPALKLQDQTG
                     KWQTLDQYKGKWVVLYFYPKDFTGGCTTQACELRDNIFAFKKADAVILGVSVDDVASH
                     EKFAKEHSLPFDILADPTKEVSGKYGVLTTYGTAQLASRQTFLIGPDGKIVKHWAKVD
                     PKGHSDMVLAEIKAHSGDKAPAKAS"
     gene            complement(3463..4686)
                     /locus_tag="VEW08_09790"
     CDS             complement(3463..4686)
                     /locus_tag="VEW08_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYJ41072.1"
                     /translation="MLIRFFTDLRAGGVPVTLPEFLSLLEALEARVAALSAEEFYYLA
                     RMAMVKDERHFDRFDRVFAEHFEGAQKLFDKLTAQLPPEWLQQLTEKLLTEEEKRRVE
                     ALGGWDKLLETLRQRLRDQQGRHEGGNKWIGTAGTSPFGNQGFNPEGVRMGQATSRHR
                     RAVKVWDARDYRNFDDEVELGTRNLKMGLRRLRRFAREGAADELDLDGTIDATARNAG
                     LLDIKLQPERRNAVKLLLLLDVGGSMDPHIKVCEELFSAARAEFKHLEYFYFHNFPYE
                     RVWKDNRRRFSVITDTWSLLRTFNPDYRVVFVGDATMSPYEIEQAGGSVEHWNEESGR
                     VWMTRIAAHFPRLMWLNPESQDRWEWTPSVAITRELISDRMFPLTLEGLDRAMRELKR
                     SGSAYTQLHAPVPRT"
     gene            complement(4714..5568)
                     /locus_tag="VEW08_09795"
     CDS             complement(4714..5568)
                     /locus_tag="VEW08_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002049937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYJ41073.1"
                     /translation="MTETRFTGTERYVATPDLMMAVNAAVTLGRPLLIKGEPGTGKTQ
                     LAAEIATALQRPLYEWHIKSTSKAHHGLYEYDAVSRLRDSQLGEEKVHDIRNYIMKGS
                     LWEAFESAVQPVLLIDEIDKADIEFPNDLLRELDRMEFFVHETRELIKATHRPIILIT
                     SNNEKELPDAFLRRCFFHYIRFPDAETMEKIVRVHYPSLKSELLREALTTFFDVRETP
                     GLRKKPSTSELLDWIKLLLAEDIPPEALRGDDPKKTIPVLHGALLKNEQDVHLFEQLV
                     FLNRRGSR"
     gene            complement(5629..>6715)
                     /locus_tag="VEW08_09800"
     CDS             complement(5629..>6715)
                     /locus_tag="VEW08_09800"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HYJ41074.1"
                     /translation="ACVALPALLYWGLAPNFDALELPAIVAGTGTTPAAAAIIAADAL
                     PVGLFVSIYLAVMLLLLGRLAVGLAGTWRISRAARPMALGDDVRISARIRSPATFGAI
                     ILLPVGASAWPSERLDAVLAHERAHVRSRDGYWSWLAQLHVAIFWFSPLSWWLQRRLE
                     VLAETTSDDAVVAARHDPVAYAALLLDFARHPNSRSVAMSVAESNISQRIERLLARTP
                     PAGTLPRIARWAAVSLLIPVVVFAASTTNTRAEPPAQTASMPVTAGPAPTPAAAAVRL
                     SMPVNPDDYYPAVALHEMVSGSVVVEVDVDALGQLVDARVVKVEPADPRFGFADAALQ
                     VARNNKYVNPGQQPASMRFMVKFALAT"
BASE COUNT         1188 a         2166 c         2199 g         1162 t
ORIGIN      
        1 ctacgaacac gacaccgccg gcggcctcat gaacaccgat acggtgagcg tgcgtgccga
       61 cgtcacggtg gaagtcgtgc tgcgctacct gcgcatgcgc ggcgatctgc cggacaagac
      121 cgaccgcctg ttcgtagtcg atcgcgacga tcgattcctc ggcaccctgg cactcacccg
      181 gctgctgacc gaagacccgg aaaagccggt cggtgacatc ctcgatccgg aaggctcgcg
      241 gatttcgccc gaaaccaatg cccacgacgt ggtgcagctc ttccaggatc gcgacctggt
      301 gtccgccgcg gttgtcgacg agcacggcaa gctgctgggc cgcatcacgg tcgatgacgt
      361 cgtggacgtg atccgcgagg aagccgcgca cgaagtcatg tcggcggccg gcctcggcga
      421 cgaagaagac gtcttcgctg gcgtcattcc ctccacgcgc cggagactgg tgtggctggg
      481 gatcaacctg gtcaccgcct tcatggcggc ggccgtcgtc agtcgcttcg aaggcaccat
      541 cgaaaaggtc gccgcgctgg ccgcgctcat gcccatcgtc gcctccatgg gcggcatcgc
      601 cggcacgcag accgtgacgc tgatcatccg tggcatcgcg ctcggtcagg tcgagtgggt
      661 caacgcgcgc tggctgttgt ggaaggagat tgccgtcggt gggctcaacg gcctcatctg
      721 ggcggcggtt gtcggggcgg tcaccatcgt ctggtatggc acgtggaaaa tcgccgccat
      781 catcgcggcc gccacgctca tcaatctgct ggccgccgcc gccgtgggtg tcatcgtgcc
      841 gctcgtgttg cggcggctgc gcatcgatcc ggcgctgtcg gcgggcgtga tcctgaccac
      901 cttcaccgac tgcatcggct ttgcgacgtt gttagggctg ggcgcgctgt tcctgacctg
      961 agggcggcgc gtcaccgggg cgccattcga atttctccac cgcgacgccc tgcgtcgctc
     1021 ccggcgcgcg cagcgcgagc gcgagcacat gctcttgcgt cggtgcgtat tgccagaacc
     1081 gccagtccgc gacgtcgccg gcggcgaacg acaagccgac gacccggtga tttgcgtcga
     1141 gcgagaattc ggcattgtcg agtcccgcga cgaggccctt tccggtcgcc ttcatccacg
     1201 actctttcaa tgtccacaac gcatagaagc gcgccagctg ctcagggatc ggcaacgact
     1261 cgagattgcg tgcttcatcg ggcgtgaagt agcgacgcgc gagcttcaac gccgtagtgc
     1321 gcgcgtggag attctctgcg tcgatgccca cgtctcgcag ccggctcacg gcgacagcga
     1381 tgaagccatc ggtgtgagtg acgttgaagt ggagccccag ggattcgaaa ggcggagcca
     1441 gtgccggctt cccatgctca cccttcacga atcgcaggct cgcgggatcg acatccggcg
     1501 cgtagcggcc gagcacggtc cgctgcatga cgcgcgcgcg cagaaaacgc aggcgcacct
     1561 tcggcgaata cgccttgtcc acgcgctcga gctccgcggg ctcgagtagc ccccgccgcg
     1621 cctcgagcgc cgcctcgtcc cccgggcgct cagccgactg ccatacgtgg atttcggcgg
     1681 gccccagatt cacgattcag gcgagaaacg cggcgagctc gtcgcgcttg ccgagcgcgt
     1741 cgcgatatcc catctcgatg agttcgcgcg tgtaacccga ttcgaacatc aggtagctcg
     1801 caagcaggct gcccgccgca ccgcgcgcgc cgatgattcg catgagtgag cgcaggctca
     1861 cgggcagcga gtcgaggtgc cggaccgcga tctgcgaagg atcgacgctc ggggcgatca
     1921 gcatggtgcg gatctggcgc gtgccggtca cgccgccggc gagttccgaa agccggttga
     1981 tgcggttcag gttctcgaga tcggcggaaa tctggtcgct gaaaagcgtg tccagcatgt
     2041 agccgaacaa ctgccctggt gtcggaggtg aatccgatgc gacgaagcgc gggatcaggt
     2101 cgaggtgcgt cggacgcacg cccagcacca gcacgcgatc ggctcccagg tgaatcgccg
     2161 gactgagcgg cgcggtctgg cgcatcgcac cgtcaccaaa gtattcgccg tgcaggcaca
     2221 tcgggggaaa caggaacggg ataccgagac tggccatgag gtagtcgagc gacagcggca
     2281 cacgttcacc atgccgttgt gccctcgacc attcgctaat ggtggtctcg gcttcgaaaa
     2341 acgaaactga gcgcgcggtg aagtaactgg tggcgcacaa cgccagcgcg cgcaagtcac
     2401 ctcgcgccac gttcgcgcgg atggcatcga aatccgcgac cttggcgaga agctcgcgca
     2461 gcggcgagtt gtcgagcagg gatttcggcg ggccgatcat gccgccggtg aacagggaac
     2521 ccatccagcg ggcgcctgct ctaagcatgg ctatggcgtc ggagcggaat acctgggcca
     2581 cgtggaaatc tgcccagacc ttctcgatct cacccacgga ctgatgccat cgcgtgacat
     2641 tgcctgccag gatgctggcc gaaacggccc cggcagaagt gcccacgatg atggggaatg
     2701 gccattcggg gctggcatgc atttcggcga gcgccttgag cacgcccacc tggtaggcgg
     2761 cacgcgcgcc gccgccggtc aggatcagac ccgcgcgccc cacatttgcc ttgctagaat
     2821 ccgcttcccc cacggcccat ccattttcac ggagaacaac atgctcgcac ttcgaaggac
     2881 attggtcacc gccctcatcg cagtgagtgc cagtgtatgt gcccaggctc ctgccgcctc
     2941 acccgcggaa ggcacagcgg cgcccgcgct caaactacag gatcagacgg gcaagtggca
     3001 gacgctggac caatacaaag gcaagtgggt ggtgctctat ttctacccga aggatttcac
     3061 cggcggctgc accacccagg cctgtgagct gcgcgacaac atcttcgcgt tcaagaaggc
     3121 cgacgcggtc attctcggcg tcagcgtcga cgacgtcgcc tcgcacgaaa aattcgccaa
     3181 ggaacacagc ctgccgttcg acatcctcgc ggatccaacc aaggaagtgt ccggaaagta
     3241 tggcgtgctg accacctatg gcaccgctca actcgcgagc cgccagacgt tcctcatcgg
     3301 ccccgacggc aagatcgtca agcactgggc gaaggtggat ccgaaaggcc actcggacat
     3361 ggtgctggca gagatcaagg cccattccgg ggacaaggcg cccgccaaag ccagttgatc
     3421 gagagcggtg ggaggtgcgc ggctccgcca cctcccacgc attcaggtcc gaggcactgg
     3481 agcgtgtagt tgggtgtacg cgctgccgct gcgcttcaac tcgcgcatgg cgcggtcgag
     3541 gccctcgagg gtcagcggaa acatgcggtc gctgatgagc tcacgggtga tcgccaccga
     3601 cggcgtccac tcccagcgat cctgcgattc cgggttgagc cacatcaggc gcgggaaatg
     3661 cgcggcgatc cgcgtcatcc acacgcggcc ggattcctcg ttccagtgct ccacactccc
     3721 gcctgcctgc tcgatctcgt acgggctcat ggtggcgtcg ccgacgaaca ccacacggta
     3781 gtcaggattg aacgtgcgca gcaacgacca ggtgtccgtg atcacgctga agcggcgccg
     3841 gttgtccttc catacacgct cgtacgggaa gttgtggaag tagaagtact cgagatgttt
     3901 gaattccgcg cgggcggccg agaaaagctc ttcgcagacc ttgatgtgcg gatccatgga
     3961 gccgcccacg tcgagcagca acagcagttt cacggcgttg cgccgttccg gctgaagttt
     4021 gatgtccagc aacccggcat tgcgcgccgt ggcgtcgatg gtgccatcga gatccagttc
     4081 atccgcggcg ccttcgcgcg cgaaacggcg caggcgccgc aagcccatct tgagattgcg
     4141 tgtaccgagc tcgacctcgt cgtcgaagtt gcgatagtcg cgcgcgtccc agaccttcac
     4201 cgcgcgccgg tggcgggacg tcgcctggcc catgcgcacg ccctcggggt tgaatccctg
     4261 gttaccgaaa ggcgacgtgc ctgccgtgcc gatccacttg ttaccgccct catgcctccc
     4321 ctgctgatcg cgcaggcgct ggcgcagcgt ctccagtagt ttgtcccagc cgccgagcgc
     4381 ttccacgcgg cgcttttctt cctcggtcag cagcttctcg gtgagctgtt gcagccattc
     4441 gggtggcagt tgcgcggtca gcttgtcgaa cagcttctgg gcgccttcga agtgctcggc
     4501 gaacacgcgg tcgaagcggt cgaaatgccg ctcgtctttc accatcgcca tgcgcgccag
     4561 gtaatagaac tcttcggcgg acaacgccgc gacccgggct tcgagcgctt cgagcagcga
     4621 gagaaattcg ggcagcgtca cgggtacgcc gccggcgcgc agatcggtga agaagcggat
     4681 cagcacgatg cgcccggcca gtgcaccgca ggttcagcgg cttccgcggc gattgaggaa
     4741 caccagctgt tcgaacaggt gtacgtcctg ctcgttcttg agaagcgccc cgtgcaggac
     4801 cggaatcgtt ttcttcgggt cgtcgccgcg cagtgcttcg ggcggaatgt cttccgcgag
     4861 cagtagtttg atccagtcga gcagctcgga ggtcgacggc ttcttgcgca ggccgggcgt
     4921 ctcgcgtacg tcgaagaacg tggtcaacgc ttcgcgcagc aactcggatt tgagcgatgg
     4981 atagtgcacc cgtacgatct tctccatcgt ctcggcatcc ggaaagcgga tgtagtgaaa
     5041 gaaacagcgg cgcaggaacg catccggcaa ttccttctcg ttgttgctgg tgatgaggat
     5101 gatgggccgg tgagtcgcct ttattaactc gcgcgtctcg tgcacgaaga actccatgcg
     5161 atcgagctca cgcagcaggt cgttggggaa ttcgatgtcg gccttgtcga tctcgtcgat
     5221 gagcagcacc ggctgtaccg ccgactcgaa tgcctcccac aggctgccct tcatgatgta
     5281 gttgcggatg tcgtgcactt tttcttcgcc gagctgcgag tcgcgcagtc gcgagacggc
     5341 gtcgtattcg tacaggccgt ggtgagcctt gctggtggac ttgatgtgcc actcgtacag
     5401 cggccgctgc agcgctgtgg cgatctccgc ggcgagctgg gtcttgcccg tccccggttc
     5461 gcccttgatg agcagcggcc tgccgagggt cacagcggca ttcaccgcca tcatgagatc
     5521 gggcgtggct acgtagcgtt cggtgccggt gaagcgggtt tcggtcatgc ccgcgagctt
     5581 atgccgtgcc gggtggcggt ggccacccgg caggcgctcg agcgacgctc acgtcgccaa
     5641 tgcgaacttc accatgaacc gcatggacgc tggctgctgc cccggattca cgtatttgtt
     5701 gtttcgcgcg acctgcagcg ccgcgtcggc gaagccgaag cgagggtccg ccggctccac
     5761 tttcaccacc cgggcatcca ccagttgccc gagtgcatcc acgtcgactt ccactactac
     5821 agatcccgac accatctcgt gcaaggcgac cgccggatag tagtcgtccg ggttgaccgg
     5881 catgctgagg cgcacggccg cggctgcggg cgtcggcgcc ggacctgccg tcacgggcat
     5941 tgaggcggtc tgcgccggtg gctcggctcg cgtgttcgtg gtcgaggccg cgaacacaac
     6001 gaccgggatc agaagcgaga cggcggccca gcgcgcaatg cgcggtaatg tgcccgcggg
     6061 tggggtgcgg gcgagaagac gctcgatgcg ttgggaaata ttcgattctg cgacggacat
     6121 ggcgacgctc ctggagttgg ggtgacgggc aaaatcgagc aacagcgcgg cataggccac
     6181 ggggtcgtgg cgcgcggcga cgaccgcatc gtcgctggtg gtttccgcca gcacttcgag
     6241 ccggcgctgc agccaccagg aaagcgggct gaaccagaag atggccacgt gaagttgcgc
     6301 gagccacgac cagtagccat cgcgcgaccg tacgtgcgcg cgctcgtgcg cgagcacggc
     6361 atcgagacgc tccgacggcc acgccgacgc ccccaccggc agcaggatga tggcgccaaa
     6421 ggtcgccggg ctgcgaatgc gagcgctgat gcgcacgtcg tcgcccagcg ccatcggccg
     6481 tgcggcgcgg ctgatgcgcc atgtgccggc gagaccgacg gcaagacgcc cgagaagcag
     6541 cagcatcacc gcgaggtaga tagacacgaa caagccgacc ggtagcgcgt cggccgctat
     6601 gatggccgcc gccgcgggcg ttgtgcccgt gccggccacg atggcgggca gctcgagcgc
     6661 gtcgaagtta ggggccagcc cccagtacaa cagcgccggc agagcgacgc acgcg
//
LOCUS       DASQJS010000242        14936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1083457, whole genome shotgun sequence.
ACCESSION   DASQJS010000242 DASQJS010000000
VERSION     DASQJS010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14936
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083457"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="VEW08_09805"
     CDS             complement(<1..1293)
                     /locus_tag="VEW08_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41075.1"
                     /translation="MIRKAFIAAALLAFAGGANAATDYPSGYTKCAKDGETCTFSGTR
                     SVAYGKSGTFVYANLTGPITCSASLFPSISVATPRYCSYGAVVTPPVCPATTLGPVIN
                     GAQTASATVASGASVAFSPTPTSGGSWSWSGCGTSGTARAQTVFPTASCSATATHTNS
                     CGTVSTLVHTITVNAPSCPATTITAVINGTQTASATINSGASVAFAPLPSSGGSWSWS
                     GCGTSGNARTQTVSPTASCTATATYTNSCGTQSTRAHTITVNGTTQPPSGTPIGFGRS
                     TTGGAGGATVTVSTGTQLNTALCSRASVSTPIIIRVNGTINHGNTTKVSGSCDTTATE
                     VELKGVSNISIIGVGTSGVLDQIGLHIRNSSNIIIRNLHIKNVKKSGSPTSNGGDSIG
                     METSVNNIWIDHNTLEASGGESDGYDSLLDMKAGVTNVT"
     gene            1749..2573
                     /locus_tag="VEW08_09810"
     CDS             1749..2573
                     /locus_tag="VEW08_09810"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ41076.1"
                     /translation="MLKEETFTGRGGTRIFMRSWRPPAPKGVVVICHGVNSHSGQYLW
                     TGEQLAAAGFAVYAYDHRGRGKSEGPRFYIDDISDYTEDLGTLINLAKSREPGLKVFL
                     LGHSAGGVVGCTWALDHQQEIAGFICESFAYKVPAPAPVLALVRLFGKIAPRLPVLKL
                     HMKDFTRDPAALATLEADPLCKGEIQPARTVAALLKATDRMKAEFGTLKLPLLIMHGT
                     NDKATVPAGSKEFYEAAGSSDKTLKLYQDHYHDLLNDFGKDKVMTDIVLWVNTHLA"
     gene            complement(2577..3260)
                     /locus_tag="VEW08_09815"
     CDS             complement(2577..3260)
                     /locus_tag="VEW08_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HYJ41077.1"
                     /translation="MLRRAGFRIRYVNFSRQPDQRPDVSRYNGLVVLGGPMNVDQAER
                     FPHLTAEITAIQQALALGIPILGICLGAQLLAAALGAHVRPNNVREIGWYRLHPTAAA
                     GGDPLCRHLGGDQHVFQWHAYTFDLPPGAVHLASTPTCPNQAFRFGDRAYGLQFHLEA
                     DEDLIQRWLHVPEYRTEAEASGPEHRLEHILRDTHSHAPHALTVSERVFGEFIELLGW
                     KRRVRLGSL"
     gene            3358..5376
                     /locus_tag="VEW08_09820"
     CDS             3358..5376
                     /locus_tag="VEW08_09820"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HYJ41078.1"
                     /translation="MIREWFGKSGFSLRTGFWILASAAFYYLATRTAWVLCFPDSKVS
                     LFFPPHAVLVSILLLVPTRHWWAYTLAAVCAHFLATQQEHWPPLYALQCEAFDAVKAI
                     LTAVGIRLFIKSPFHLISLREAVIFVLIAVIITPALTSLWGAAFTVANNYGTRYWVEW
                     RNLSISNGVTTIVLVPAILIGVNQLLTKGFKVAPGRILEACLLAAGLLAVGWLAFDRS
                     PAGPDTSPALIYAPVPLLVWAALRFRLGGMSASILVITMLAIWGTMQGRGPFLTQTPA
                     ENALALQLFILMAATPLLLLAVAIDDERRSKEALRVSEERMSLAVESAQLALWEWDVA
                     NDRVWIQDEGLFGFDPRTPVDHATLAGHVHPDDRATRMAAIQRALATGGSYEAEFRII
                     LPDGSVRWIAARGRSPSVTTNPLRLLGVALDITRQKQASAEASLQREELAHLSRVATV
                     SALTGSLAHELSQPICSILNNAQAAQSFMSRDPPDYTELETILAEIVTADLRAGDIIE
                     RLRTMLRRGEVALQPVSVKEILEEMLRLTRTDLIGRGVSVRNLTTSDLPPAMTDRVQL
                     QQVLLNLIVNACDAMETNPPDDRILTLTSFIENDEVRIGVLDCGIGLPADIEILFQPF
                     HTTKENGLGLGLAICRSLLAAHGGRLWAERGSKRGAAFYVALPLATRV"
     gene            5373..5849
                     /locus_tag="VEW08_09825"
     CDS             5373..5849
                     /locus_tag="VEW08_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYJ41079.1"
                     /translation="MTGHTSQDPELLRRLLRAKDRMDVASHEEWPVERLARVSRVSKA
                     HFARSFKDAFGVPPHRYLLTRRIERARTLLRDTELSITDIAFQTGWKSLGTFGRIFRD
                     ITGESPGQLRTREKATPHELDRVPACYLSAAHRPNLINAVSEKRRREAKVKNKATR"
     gene            5859..6275
                     /locus_tag="VEW08_09830"
     CDS             5859..6275
                     /locus_tag="VEW08_09830"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ41080.1"
                     /translation="MSQGVGIVGLYVRDQDEALKFYVEKLGFRVHTDSRNGDYRWLTV
                     QHPDQPSFQLGLYKPGPPVLDAATAQTLSEIVAKGAMPPLVLVVDDCRVAYKRMHAAG
                     VEFTQEPVTRYGSTDASFRDPSGNGWKLIEARKGAA"
     gene            6272..6469
                     /locus_tag="VEW08_09835"
     CDS             6272..6469
                     /locus_tag="VEW08_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41081.1"
                     /translation="MKPSAFIRQFHRWVSMAFTVTVIANFVAMSQGTPPAWITYSPLL
                     PLALLLLTGLYLFALPYLSRR"
     gene            complement(6423..7490)
                     /locus_tag="VEW08_09840"
     CDS             complement(6423..7490)
                     /locus_tag="VEW08_09840"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HYJ41082.1"
                     /translation="MKNSDLLRLRLHNQQLSETRSRRPRDVVSWLVAMQAQEYAMAKW
                     AIGLRLPGVVSDADIERAFNAGEILRTHVLRPTWHFVAPADIRWLLELTAPRVHAASA
                     YMYRKLELTAAVFKRSDAVLVRALRSERFLTRTALQERLARSGVKAEGPRLGYLMMHA
                     ELEGLICSGPREGRQFTYALLDVRAPGARPMRRESALAELSRRYFASRGPATPHDFAN
                     WSGLTVADARAGVESLDGEFVRQTIDGRTHVFRPANVSLRSATPFAFLMPDYDEFGMS
                     YKDRRALATPGVGTLAYNRMIVVDGRIVGSWRRSLAGGSIAIETDHPARLSRARLRAV
                     SLAKRRFTAWIDTAAQTGTDP"
     gene            7532..7966
                     /locus_tag="VEW08_09845"
     CDS             7532..7966
                     /locus_tag="VEW08_09845"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HYJ41083.1"
                     /translation="MKASAGKKRVAGKAKILSGDNPQIAMGHGNAPVKAYIAAIPGWK
                     RDLARRLDSLIVRTVPNVYKAVKWNSPLYGFENQGWFLSFHCFTNYIKVTYFRGTSLQ
                     PVPPGQSKHKEIRYLDIRDGELDEAQFVAWVKQASELPGEKM"
     gene            8063..8569
                     /locus_tag="VEW08_09850"
     CDS             8063..8569
                     /locus_tag="VEW08_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41084.1"
                     /translation="MNTTAAITTPSNYRITPLRGILLGGFLAALADTIYPTVKTVMGG
                     GEWVRPWKGVASGLLGPAAREGGMEMVALGLALHYFICISAAALLYLLASRVKFVPRQ
                     WVVLGVLYGIAFLLTMNYVILPLSAIGRGIYPLEQLHIHAFWHIVLVGLPTAFFVTRA
                     LKQVAPHR"
     gene            8797..12819
                     /locus_tag="VEW08_09855"
     CDS             8797..12819
                     /locus_tag="VEW08_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HYJ41085.1"
                     /translation="MVQSFKRFAGTWLAILLVAFAPSAFAQATYYTNTDNENNAGTHV
                     ADNDMDISLGNASLIHPVEFNINVATLPESSVVLSIRALDVDEEQGEVDNVYINGHLL
                     GKLTGANGVWSVTALNVIPAWLVAGQNLVRVNVDTSGDSTAWVTTIDWAQLLTDGGGA
                     VDGDTQSVQITGTSTTLTTVTINTSTSVHSITGGTYRLQISLIDPNNNAVTVLSEDFS
                     VAAGADVTRTASPVYPLSSVSGTYTIQAQLFWLDPSQGGFPVQQDIAIAQFTHTAGSG
                     ASNFSNDSDGDGLLDNQETTLGTDMNDADTDGDGTGDGAEVGPDVNSPVDTDGDGVIN
                     ALESSIVDTDGDGVMNQLDPANNNPCIPNASFSVCPAGDADGDGLTNAQEGVLGTSGS
                     NPDSDGDGTNDGSEVGADVNSPLDSDGDGAINALESSIVDTDLDGVSDQLDAANADGC
                     VPNANSIVCLMTDSDGDGLTNSQEDTLGTSRSDADTDGDGAGDGAEVGNVVSPVDSDG
                     DGTPNVFESSIADLDGDGVTNQSDPANANVCIPNSNSAGCLATDSDGDGLTNGQEDAL
                     GTSRGNADTDGDGTNDGAEVGGNVNSPADSDGDGNPNVLESSVADADGDGASNQNDSA
                     NGNACVPNPNSTPCLGLDNDGDGLTNSQEDALGTDRNNSDSDGDGISDGAEAGTNPAD
                     PTDTDHDGIPDVFEAGDSDNDGTPDAADVDSDNDGIADSVEAGADPMNPVDTDNDGTP
                     DYLDDDSDGDGVPDALEVGSTPSDPRDSDSDGSPDYLDTDSDNDTLPDTLEGNVNDND
                     TLLDTDGDGAPDLADVDSDDDGILDSFEGTPGALTDTDGDGVADLRDLDSDNDGISDV
                     IEGGLIDTDGDSQMDAGQTRTDNPGPGVRNPVSGGFLTGADSDEDGVRDTVDAAPQVF
                     GTFVDRDGDGVADLADLDLDNDGIPNSADGSDDTDGDGLPNLGDLDSDGDGITDIIEA
                     GGLDANGDGVADNFADVNGNGLSDSFEGSLGGHSLPLPDTDADGTDNHRDLDSDGDSI
                     SDVIESSGLDTNADGRQDGVDSDRDGLADSTDSTTGGHALARPDTDGDGMSDSLDLDS
                     DNDALPDAREGRGDFDGDGVPDSIDKQGTIQTAVRGAGAFDPLSLSGLLCLIGWAALR
                     RSGQRVAVYLLPILAVAAGLAPAQAGAADTISKGWYLGLDTGMSRLEPQNDDGGYKVD
                     DGQGFGYRLTAGYAWNQNWSVEGFFADGGDAGISSDNPGVGHLGDISYKVFGVGFQWL
                     PFDGGINEQWFPLVKFGASHIQNESSSPMVVYEKLNDLGVYFGGGAGLRFGENWVGQA
                     EVVSYDADELFFTVGIRKHF"
     gene            12890..13414
                     /locus_tag="VEW08_09860"
     CDS             12890..13414
                     /locus_tag="VEW08_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYJ41086.1"
                     /translation="MAKHFLALLLFAGLAQAEEIANPLIDYAAFQSGVERVGAVREAH
                     RVTEFEFMLMASDPSTVILDARSREKFELLHVKGAKNLSLPDMTEDDLAKVIPSKSTR
                     ILIYCNNNFLNEPKAFPSKNFSASLNVYTMNVLFAYGYTNVFELGPLIDIRSTRIPFE
                     RASRAVQPQAVQNR"
     gene            13556..>14936
                     /locus_tag="VEW08_09865"
     CDS             13556..>14936
                     /locus_tag="VEW08_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ41087.1"
                     /translation="MKKKILLATVFAVAQIAASLPAAGQTPSPQAADSGAEEFGEIVV
                     TAERRRTSLQTTPIAATVMTGEDVAASGVTTVDQLQFISPAATVNNFGQGIDFNIRGI
                     GKAEHNTQTTTGVITYRDGVATFPGYFTAEPYYDISRVEILRGPQGTFVGQNATGGAV
                     FVTSQDPVINGGHDGYLQAQAGNYNSFGAQGAINLPAGDTFAARIAFNGDTRDSVYDI
                     SGPYTGDEGVKTGSIRLGMLWEPSEKLSILWKTDYNYLDLSGYPADPVNATNGMFDIT
                     ANAEIMGRDRFGRSVLKIDYEFGNGTSLRSVTGYQEGQTEYRADLDGTSTGNNIFGDA
                     VDETIFSQEINLISSDAGKFQWILGAYYQHDEYEFPPGEFYIGIPAGFYLLDGTNPKE
                     TSALFGQVSFGLAEGWELEIGARYSESSTSNDINVNQYGLPLTQDQEAEFTNLSGKVS
                     LNWEVNPHHF"
BASE COUNT         2885 a         4654 c         4667 g         2730 t
ORIGIN      
        1 cgtcacgttg gtcacgccgg ccttcatgtc gagcagcgag tcatagccat ccgattcacc
       61 accgctggcc tccagcgtgt tgtggtcgat ccagatgttg ttgaccgagg tctccatgcc
      121 gatcgaatcg ccgccgttcg aggtgggcga accgctcttc ttcacgttct tgatgtgcag
      181 gttgcggatg atgatgttcg acgagttgcg gatgtgaagg ccgatctggt cgagcacgcc
      241 cgaggtgccg acaccgatga tcgagatgtt gctgacaccc ttgagttcca cttccgtggc
      301 cgtggtatcg cagctgccac tcaccttggt ggtgttgcca tggttgatcg tgccattcac
      361 acggatgatg atcggcgtgc tcacgctcgc gcgactgcaa agcgccgtgt tgagctgtgt
      421 gccggtgctg accgtcaccg tcgcgccgcc cgcaccgccc gtggtcgaac gtccgaagcc
      481 gatcggcgtg ccggacggag gctgagtcgt gccgttcacc gtgatcgtat gagcccgggt
      541 cgactgcgtt ccgcagctgt tggtgtatgt cgccgtcgcc gtgcaggacg cagtcgggct
      601 cacagtttgg gttcgcgcgt tgccggacgt gccgcaacca gaccatgacc agctaccgcc
      661 ggacgacggc agcggagcga aggctactga tgcaccagaa ttgatagtgg cgctggcagt
      721 ctgggttccg ttgatgacag cagtaatggt cgtggcgggg cagctcggtg cgttcaccgt
      781 gatcgtgtga accagggtcg agactgtgcc gcagctgttg gtgtgcgtgg cagtcgccga
      841 gcacgaggca gtcggaaata cggtctgcgc gcgcgcggtg ccggaggtgc cgcagccgga
      901 ccatgaccag ctaccgccgg aagtcggcgt cggagagaag gccaccgacg caccggaagc
      961 caccgtcgcg ctggcagtct gtgcgccgtt gatgaccggg ccgagggttg tggccgggca
     1021 gaccggcggt gtcacgactg ccccgtacga gcagtaccgc ggcgtggcta cgctgatgga
     1081 agggaacagc gaagccgagc aggtgatcgg gcccgtgaga ttagcgtaaa cgaatgtgcc
     1141 tgacttgccg tacgccacgg agcgcgtgcc gctgaaagtg caggtctcgc cgtctttggc
     1201 gcatttcgta tagccacttg gatagtcggt agccgcattg gcgccgcctg caaaagcaag
     1261 caacgcagcg gcgatgaacg ccttgcgaat catgaagaat cccccttgtt tgtatgtcgt
     1321 tccatcaacg ggaaactgtc agccggccgc ggaaggcagc cgacaccgtg tcactcgata
     1381 gaaacgcctt cctgacaccg gtgtcaggcc gagagtagca cacggctgga aaaggacatc
     1441 tttgttagcg ctactagggc atgaaacggc cgttttcagg acatattctt atcatctggt
     1501 cgcaaagtcc tacgattctg atcaaggaag tccctgtgtg acaccggtgg tcaggttttg
     1561 ggtgtcccgg cggcctggcg ggagtgggag gtgctacctg ctcgcaatct cctaacaact
     1621 cgcttcgcgc aggccaaatt gaagcacccg ctggcgtatt gcctgacgat ggtgcaagcc
     1681 cacaaggcgt attgacgcaa tggaattgca tgactagttt cgaagcgatt cgttactggg
     1741 gaatgccaat gctcaaagaa gaaactttca ccggccgcgg tggcactcgt attttcatgc
     1801 gttcctggcg gccacccgcg ccgaagggag tggtggtgat ctgccacggc gtgaactcgc
     1861 acagcggcca gtatctatgg actggtgagc agctcgccgc agccggcttc gctgtgtatg
     1921 cctacgatca tcggggtcgc ggcaagtccg agggaccgcg cttctatatc gatgacatct
     1981 cggactacac cgaagacctg ggcacgctga tcaacctggc caagtcgcgc gaacccgggc
     2041 taaaggtgtt cctgttgggc cacagcgcag gcggggtggt ggggtgcacc tgggcgctgg
     2101 accatcagca ggaaatcgcg ggctttatct gcgaaagctt tgcgtacaag gtgccggctc
     2161 ccgcgccggt gctggcgctg gtacggctgt tcggcaagat cgcgcccagg ctgccagtgc
     2221 tgaagctgca catgaaagac ttcacgcgag atccggcggc cctggccacg ctcgaagccg
     2281 atccgctatg caagggcgag attcagccgg cacgcacggt ggcggcattg ctcaaggcga
     2341 ctgaccgcat gaaggcggag ttcggcacgc tgaaacttcc gctgctcatc atgcacggca
     2401 cgaacgacaa ggccaccgtg ccggcgggca gcaaggagtt ctacgaggcc gcgggctcca
     2461 gcgacaagac gctgaagctc tatcaggacc attaccacga tctgctgaac gacttcggca
     2521 aggacaaggt gatgacggac atcgtgttgt gggtcaacac gcacctggcc tgatcgtcac
     2581 aagctcccaa gccgcacgcg ccgcttccag cctaacaact cgatgaactc gccgaacacg
     2641 cgctcactca ccgtgagcgc gtgcggcgca tggctgtgcg tgtcgcgcag gatgtgttcc
     2701 agacggtgtt cggggccgga cgcctccgcc tcggtccgat attcgggcac gtgcagccag
     2761 cgctggatga gatcctcatc cgcttccagg tggaactgca gtccgtacgc gcggtcgccg
     2821 aaccgaaacg cctggttggg gcaggtgggc gtgctcgcca ggtgcacggc gccgggcggc
     2881 agatcaaagg tataggcatg ccactggaac acgtgctggt ccccaccgag gtggcggcaa
     2941 agtggatccc cgccggccgc cgccgtggga tgcaggcgat accagccgat ctcgcgcacg
     3001 ttgttgggtc gcacgtgggc gcccagcgcc gcggccagca gttgcgcgcc gaggcagatg
     3061 cccaggatgg gaatgcccag cgccagcgct tgctggatgg cggtgatttc ggctgttagg
     3121 tgcggaaagc gctcggcctg gtcgacgttc atcggcccgc ccaggacgac gaggccgttg
     3181 tagcgactga catcgggccg ctgatcgggc tggcgcgaaa aattgacgta gcggatgcgg
     3241 aatccggcgc ggcggagcat aggatcgaag acgccgagtg gttcgcgggc cgagtgttga
     3301 aagaccagga gcttgcgcat gcggccaaca aaatagcaga ggggatatgc tgtcggcatg
     3361 atcagggagt ggtttgggaa aagcgggttc tccctgcgca ccggattctg gattctggcc
     3421 agtgccgcct tctattacct ggcgacgcga accgcctggg tgctgtgttt tcccgacagc
     3481 aaagtctccc tgttctttcc gccgcacgcg gtcctcgttt ccatactgct gctcgtgcct
     3541 acccggcact ggtgggctta cacactcgcc gccgtttgcg ctcacttcct ggccacgcag
     3601 caggagcatt ggccgcctct ctatgcgctt caatgcgagg cattcgatgc cgtgaaagcg
     3661 atcctgacgg ctgtcgggat tcgcctgttc atcaagtcgc cgttccatct catcagcctt
     3721 cgcgaagccg tcatctttgt actgatcgcc gtgatcatta ctccggcgtt gacttccctc
     3781 tggggagccg ctttcacggt cgccaacaac tatggaactc gctactgggt cgaatggcgc
     3841 aacctcagca tctcgaacgg agtgacaacg atagtgctgg ttccggccat cctgattggc
     3901 gtcaaccagt tgctgaccaa aggattcaaa gtcgcgcccg gtcgaatcct ggaagcgtgc
     3961 ttgcttgcag cgggtcttct cgcggtcgga tggcttgcct tcgatcgctc gccagcggga
     4021 ccggacactt cgcccgcact catttatgcg cccgttccgc tgctcgtctg ggcggctctg
     4081 cgcttcaggc tgggaggcat gagtgcgtcc atcctggtca tcactatgct ggcgatctgg
     4141 ggaaccatgc agggacgcgg gccgttcctg acgcaaaccc ccgcggaaaa cgcactggcc
     4201 ctgcagttgt tcatcctgat ggcggccact ccgctgctgt tgctcgcggt cgctatcgat
     4261 gacgaacgac ggtcgaagga ggcgttgcgc gtcagcgagg agcgcatgag cctggcggtg
     4321 gagagtgcgc aattggcgct gtgggaatgg gacgtggcga atgacagagt ctggattcag
     4381 gacgaagggc ttttcggctt tgatccgcgg acaccggtcg atcacgccac tctggcggga
     4441 cacgtgcatc ccgacgatcg cgcgacgcgc atggccgcga tccagcgcgc gctggcgact
     4501 ggcggttcct atgaggctga gtttcgcatc atcctgcccg atggctccgt gcgatggatc
     4561 gccgcgcgcg gccgtagccc aagcgtcacc acgaatccgc tgcggttact cggcgtcgca
     4621 ctggacatca cgcggcaaaa acaggccagt gcggaagcga gcttgcagcg ggaagagctg
     4681 gcgcatctgt cccgcgtcgc caccgtcagc gcgctcacgg gttccctcgc gcacgagttg
     4741 agccagccaa tctgcagcat cctcaacaat gcacaggcgg cgcagagctt catgtcgcgc
     4801 gatccgcccg actacaccga gctcgagacc atcctcgcgg agatcgtcac cgcggatctc
     4861 cgcgccggag acatcatcga gcgcctgcgc accatgcttc gccgcggaga agttgcactc
     4921 caaccggtga gtgtgaaaga gattctcgaa gaaatgctgc gcctcacccg caccgatctc
     4981 attggccgcg gtgtctccgt caggaatctc accaccagcg accttccgcc ggccatgacg
     5041 gatcgcgtgc aactccagca ggtcctgctc aacctgatcg tgaacgcatg cgatgcaatg
     5101 gaaaccaatc caccggacga ccgcatcctc acgctcacca gtttcatcga aaatgacgag
     5161 gtacgcatcg gcgtgctcga ctgtggaatc ggcttgccgg ccgacatcga gatcctgttt
     5221 cagccgttcc acaccaccaa ggagaacggc ctgggtcttg gcctagcgat ttgccgctcg
     5281 ctgttggccg cgcatggtgg gaggttatgg gcggagcgcg gatcgaagcg cggtgccgcg
     5341 ttctatgtcg cgctccctct cgccacacgg gtatgactgg gcacacgagc caggatccgg
     5401 agctgctccg ccgactgctg cgcgcgaaag accgcatgga tgtcgcctcg catgaagagt
     5461 ggcccgtcga acggctggcg cgcgtgagcc gcgtctcgaa ggcgcacttc gcgcgatcgt
     5521 tcaaggatgc cttcggcgtg ccaccgcacc gctacctgct cacgcgccgg atcgagcgcg
     5581 ccaggacgtt gcttcgcgac acggagctct ccattaccga catcgcgttc cagactggtt
     5641 ggaaaagcct ggggacgttc gggcgcatct tccgcgacat caccggcgag agtcccgggc
     5701 aacttcgcac gcgcgaaaag gccactccgc acgaactcga tcgcgtgcca gcctgttacc
     5761 tgagcgccgc ccaccggcca aacctcataa acgcagtttc ggagaagcgg cggcgcgagg
     5821 cgaaggttaa aaataaggcc accaggtaag gaggtcccat gagtcaaggt gttggtatcg
     5881 tcggtctgta cgttcgcgac caggacgaag ccctcaagtt ctatgtggag aagctcggat
     5941 tccgtgtcca cacggactcc cgcaatggcg actatcgctg gctcacggta caacacccgg
     6001 accagccgtc attccagctt ggcttgtaca agcccggccc gccggtgctc gacgcggcga
     6061 cggcgcagac gttgagcgag atcgtggcga aaggcgccat gccgccgctc gtgctggtcg
     6121 tcgacgattg ccgggtggct tacaaacgca tgcacgccgc cggcgtggag ttcacgcagg
     6181 aaccagtcac tcgttacggg agcacggatg ccagctttcg cgatccgtcc ggcaacggct
     6241 ggaagctgat tgaagcgcgc aaaggtgcgg catgaaaccc agtgcgttca tccgccagtt
     6301 ccaccgttgg gtgtccatgg ctttcactgt gacggtcatc gccaactttg tcgcgatgtc
     6361 gcagggaacg cctcctgcct ggatcacgta ctcgccgttg ctgccgctcg cgttgctcct
     6421 gctcacgggt ctgtacctgt ttgcgctgcc gtatctatcc aggcggtgaa gcggcgcttg
     6481 gccagggaca ctgcccgaag cctggcgcgg ctcaaccgtg cgggatgatc cgtctcgatc
     6541 gcgatggaac cgccagccag cgagcggcgc caggatccga cgatccggcc gtcaacgacg
     6601 atcatccggt tgtatgccag cgttccaacg cccggagtcg ccagcgcacg gcgatccttg
     6661 tagctcatgc cgaactcgtc gtagtccggc atgagaaacg cgaagggggt ggcactccgt
     6721 aaactcacgt ttgcggggcg aaacacgtga gtgcgtccgt cgattgtctg tcgcacgaat
     6781 tcaccatcca gtgattccac acccgcacgc gcatcggcga ccgtgagccc ggaccagttg
     6841 gcgaagtcgt gaggggtcgc cggtccgcgg ctcgcgaaat agcgcctgct gagttcagcc
     6901 agggcggact cgcgacgcat gggccgtgcg ccaggcgcgc gcacatcgag cagggcgtag
     6961 gtgaactgcc ggccttcacg agggccgctg cagatcaatc cttcgagttc ggcatgcatc
     7021 atgagatagc cgagccgcgg accttcggct ttcactccag acctggccag cctctcttgc
     7081 aatgcggtgc gcgtgagaaa tcgctccgat cgaagcgcgc gaaccaacac cgcatcgctg
     7141 cgtttgaaaa cggcggccgt caattccagt ttgcgataca tgtaggcact ggcggcgtgg
     7201 acccgcggtg cagtcaattc aagcaaccag cggatatctg ccggcgcgac gaaatgccag
     7261 gtcgggcgca gtacatgggt acgcaggatt tcacccgcat tgaaggcccg ctcgatgtcc
     7321 gcgtccgaaa ccacgccggg caagcgcaat ccgattgccc acttggccat cgcgtattcc
     7381 tgggcctgca tggccacgag ccagctcacg acgtctcttg ggcgacggct gcgggtttcg
     7441 gagagctgct ggttgtgcag tcggaggcga agtagatcgg agttcttcat ggggcttcac
     7501 tcatgggatt gccgcttgct actgttcgct catgaaagca tctgccggca agaaacgcgt
     7561 cgctgggaag gcaaaaattc tctctggcga caatccgcag atcgcgatgg gccacggcaa
     7621 cgcgcccgtg aaggcctaca tcgccgccat cccgggctgg aaacgcgacc tcgcgcgccg
     7681 cctcgactcg ctcatcgtgc gcactgttcc caacgtgtac aaggcggtca aatggaattc
     7741 gcccttatat ggtttcgaga atcagggctg gttcctcagt ttccattgct tcacgaacta
     7801 catcaaggtg acgtacttcc gaggcacttc gctgcaaccg gttcctcccg gccagtccaa
     7861 acacaaggaa atacgctacc tcgacatccg tgatggtgag ctcgacgaag ctcagttcgt
     7921 cgcatgggtg aagcaggcga gcgaattgcc cggcgagaaa atgtgagcgg gcagactgct
     7981 cggcccggct tcgggacgaa ctcgctggcc tttcgctgcg ctgtgtccgc gcggtagtct
     8041 cttcacaacg tagaggcgaa ccatgaacac caccgctgca atcaccaccc cgtctaacta
     8101 caggatcacg ccgctccgcg gcatcctgct cggcgggttc ctcgcggcgc tcgcggacac
     8161 catctacccg acggtgaaaa ccgtgatggg cggcggcgaa tgggtgcgac cctggaaggg
     8221 cgtggccagc ggcttgctgg gaccggccgc gcgtgagggc ggcatggaaa tggtggcgct
     8281 tggcctggcg ctgcactatt tcatttgtat ctcggctgcc gcactgctgt acctcctcgc
     8341 gagccgcgtg aagttcgtcc cacggcagtg ggtcgtgctg ggcgtgcttt atggcatcgc
     8401 atttctcctg accatgaact acgtgatcct gccgctctcg gccattgggc gcggcatcta
     8461 tccactggag cagctgcaca tccacgcctt ctggcacatc gtgctggtgg gtttgccgac
     8521 ggcgttcttc gtgacgcgcg cgttgaagca ggtggcgccg caccggtagc caatcgccag
     8581 cttatgtccg ctttcacggg cccccttcca cgggggcctt ttcgcctctg cgttaaggca
     8641 attcggttct agtgtcctgc cgttatttga acctcctctc cttctccgtc ctaaggctgc
     8701 gccggctcgc cgccgatacg gaacccgtac ccgcaaaagg ttgcggcgtg accgccaagg
     8761 tcttggcggt ctggtcgaac gggagttgtc ataaacatgg ttcagtcatt caagcggttc
     8821 gcgggcacct ggttggcgat attgctggtg gcgttcgcgc cgtccgcatt tgctcaggct
     8881 acctactaca caaatacgga caacgagaac aacgcgggca cgcatgtcgc cgacaacgac
     8941 atggacattt cgctgggcaa cgccagcctc atccaccccg tcgaattcaa catcaacgtc
     9001 gcgacgctgc cggagagctc cgtggtgctc agcatccgcg cgctcgacgt ggacgaggag
     9061 cagggcgagg tcgacaacgt ttacatcaat ggtcacctgt tgggaaaact caccggcgcc
     9121 aatggcgtct ggagtgtcac cgcgctcaac gtgattccgg cctggctggt tgccgggcag
     9181 aacctggtgc gcgtaaacgt cgacaccagc ggcgactcca ccgcgtgggt cacaaccatc
     9241 gactgggcgc agctcctgac cgacggtggc ggcgcagtcg atggtgacac gcagagcgtg
     9301 cagatcaccg gcacgtcgac gactctgacc acggtcacca tcaatacttc aacctcggtg
     9361 cactcgatca ccggcggcac ttatcggctg cagatttctc tcattgaccc gaacaacaat
     9421 gcggtcacgg ttctttccga ggacttcagc gtcgctgcgg gcgcggacgt tacgcgcacg
     9481 gccagcccgg tctatccgct gtcgagcgtt tcgggcacgt acaccattca ggcgcagctt
     9541 ttctggctcg atccttcaca gggcggcttt cccgttcagc aggacatcgc gatcgcccag
     9601 ttcacgcata ccgctggatc aggcgcgagc aacttcagca acgacagtga cggcgatggg
     9661 ttgctcgaca accaggaaac gacgctcggc accgacatga acgatgccga tacagatggt
     9721 gacggcaccg gcgacggcgc cgaagtcggc ccggacgtca attcaccggt ggataccgat
     9781 ggcgatggcg tcatcaatgc gctcgagtcg tcgatcgtcg acaccgacgg cgacggcgtg
     9841 atgaatcagc tcgatcccgc caacaacaat ccgtgcattc ccaacgccag tttctcggtc
     9901 tgcccggcgg gcgacgccga tggcgacgga ctcaccaacg cgcaggaagg cgtattgggc
     9961 acgagcggca gcaatcccga ctccgatggt gacggcacga acgatggctc cgaagtcggc
    10021 gctgacgtga actcaccgct cgactccgat ggcgatggcg cgatcaacgc gctcgagtcc
    10081 tcgatcgtcg atacggacct cgatggcgtg tccgaccagc tcgacgcggc caacgctgac
    10141 ggttgcgttc ccaatgccaa ctcgatcgtc tgcctgatga cggacagcga cggcgatggc
    10201 ctcaccaatt cacaggaaga cacgcttggc accagccgca gcgatgccga caccgacggt
    10261 gacggcgctg gcgacggcgc cgaagtcggc aatgtcgttt caccggtgga cagcgatggc
    10321 gacggcacgc cgaacgtgtt cgagtcttcg atcgcggacc tcgacggcga cggcgtgacc
    10381 aaccagagcg accccgccaa cgccaacgtc tgcattccga actcgaacag tgcgggctgc
    10441 cttgcgacgg acagcgacgg tgacggcctg accaacggcc aggaagacgc gctgggcacg
    10501 agccgcggca atgcggacac cgatggtgac ggcacgaacg acggcgcgga agtcggcggc
    10561 aacgtcaact ctccggcgga cagcgatggc gacggcaacc cgaacgtgct cgagtcgtcg
    10621 gtggcggacg cggacggcga cggcgcgtcc aaccagaacg attccgccaa tggcaacgct
    10681 tgcgtaccga atccgaacag cacgccttgc ctggggctgg acaacgatgg cgatgggctg
    10741 acgaactcgc aggaagatgc gctcggcacc gatcgcaaca actccgactc cgacggcgat
    10801 ggcatcagcg acggtgccga agcgggtacc aacccggcgg acccgaccga caccgatcac
    10861 gatggcatcc ccgacgtctt cgaagccggt gacagcgaca acgacggtac gcccgacgcg
    10921 gccgatgtcg acagcgacaa cgacggcatc gccgatagcg tcgaagccgg cgccgacccg
    10981 atgaatccgg tcgacaccga caacgacggc acgcccgact acctggatga cgacagcgat
    11041 ggcgatggtg ttccggatgc gctcgaagtg ggttccaccc cgtctgaccc gcgcgacagc
    11101 gacagcgacg gttcgcctga ctacctggat accgacagcg ataacgacac gctgcccgac
    11161 acgcttgaag gcaacgtgaa cgacaacgac accttgctcg acacggatgg cgatggcgcc
    11221 cccgacctgg cggatgtgga ttccgatgac gacggtatcc tcgattcgtt cgaaggcacg
    11281 ccgggcgcgc tcaccgacac ggatggtgac ggcgttgcgg acctgcgcga cctggattcc
    11341 gacaacgatg gaatctccga cgtcatcgaa ggcggcctca tcgacaccga tggcgactcg
    11401 cagatggatg ccggccagac gcgcacggac aatccgggcc cgggcgtccg caaccctgta
    11461 tcgggtggct tcctcacggg cgccgacagc gacgaagacg gtgttcgcga cactgtcgat
    11521 gccgcgccgc aggtgttcgg cacctttgtc gatcgcgacg gcgacggcgt ggccgacttg
    11581 gcggatctcg acctcgacaa cgacggcatt ccgaattccg cggacggctc tgatgacacc
    11641 gatggcgacg gcctgccgaa tctcggcgac ctcgacagcg acggcgacgg catcaccgac
    11701 atcatcgaag ccggtggcct cgatgcgaac ggcgatggcg tcgcggacaa cttcgccgac
    11761 gtcaatggca atggcttgtc ggattcgttc gaaggctcgc tgggcggtca ttcgctgccc
    11821 ttgccggata cggacgccga tggcaccgac aaccaccgcg acctcgacag cgatggcgac
    11881 agcatcagcg acgtcatcga aagcagcggt ctcgatacga acgccgacgg ccgccaggac
    11941 ggcgtcgact ccgatcgcga tggcctggcc gactcgaccg atagcaccac gggtggccat
    12001 gcactggcgc gccccgacac cgatggagat ggtatgagcg attcactcga tctcgacagc
    12061 gacaatgatg cgttaccgga tgctcgcgaa ggccgtggcg acttcgatgg cgacggcgtt
    12121 ccggattcga tcgacaagca gggcaccatc cagaccgcgg tgcgtggcgc gggagcgttc
    12181 gatccgctga gcttgtcagg tttgctgtgc ctgatcgggt gggcggctct gcgccgcagt
    12241 ggccagcgcg tcgcggtgta cctgttgccg atcctcgccg ttgccgctgg gcttgcaccg
    12301 gcgcaggccg gcgcggccga tacgatcagc aagggctggt atctcggcct cgacaccggc
    12361 atgtcgcgcc tcgagccgca gaacgacgat ggcggataca aggtcgatga cggccagggc
    12421 tttggatatc gcctcacggc aggttatgcg tggaaccaga actggagcgt cgaaggcttt
    12481 ttcgccgacg gcggcgacgc gggcatttcg tctgacaatc cgggcgtcgg ccacctgggc
    12541 gacatcagct acaaggtgtt cggcgtaggg ttccagtggc tgccgttcga tggcggcatc
    12601 aatgaacaat ggttcccgct ggtgaagttc ggcgcttcgc acatccagaa cgaaagcagc
    12661 tcgcccatgg tcgtctacga aaaattgaac gatctgggcg tgtatttcgg tggcggcgcg
    12721 ggccttcgct tcggagaaaa ctgggtaggc caggccgaag tcgtgagcta cgacgccgac
    12781 gagctgtttt tcacggtggg gattcgcaag cacttctaat aaggtaagtc cgcgggcact
    12841 ttcaccgggt gcccgcgtcc aacctgcatc tggaaccccg gaggcagtcg tggccaagca
    12901 tttcctggct ctattgttgt ttgcaggcct ggcgcaggca gaggagattg ccaatccgct
    12961 catcgactac gcagccttcc agagtggcgt cgagcgggta ggcgccgtcc gcgaggcaca
    13021 tcgcgtgacg gaattcgagt tcatgctgat ggccagcgac ccgtcgacgg tgattctcga
    13081 tgcgcgaagc cgcgagaagt tcgaactact gcacgtgaaa ggcgcgaaaa acctgagcct
    13141 tccagacatg acggaggatg accttgcgaa ggtgattcct tcgaagtcca ctcgcatcct
    13201 catctattgc aacaacaact tcctcaacga gccgaaagcc tttccatcca agaatttcag
    13261 cgcgtcgctc aacgtgtaca cgatgaatgt gctcttcgcg tacggctaca cgaacgtttt
    13321 cgaactgggt ccgctcatcg atatccgcag tacccgcatc cccttcgagc gagcctctcg
    13381 ggctgtccaa ccccaagcgg ttcaaaatcg ctaaatagat caaaagctta cgagcgcatt
    13441 taccccgtaa agatattcac acgcgtgtga gtgagtgttg acagacgcgt gcggcagtgg
    13501 ctcactccgc gcaccgcatc ggcacttgcg cgcggtgcga agagggggaa agcgcatgaa
    13561 gaagaaaata cttctcgcca cggtattcgc cgtagcgcag atcgccgcat ccttgcccgc
    13621 agcgggccag acgccgtcac cacaggccgc cgactcgggt gcggaagaat ttggcgagat
    13681 cgtcgttacc gccgaaaggc gccgcaccag tctgcagacg actccgatcg cggcgaccgt
    13741 catgaccggc gaggacgtcg ctgccagcgg cgtcaccacg gtcgaccagc tgcagttcat
    13801 ctcgccggcg gcgacggtga acaatttcgg ccagggcatc gatttcaaca ttcgcggcat
    13861 cggcaaggcc gagcacaaca cgcagaccac caccggcgtg atcacttacc gcgatggcgt
    13921 ggcgacgttt ccgggctact tcaccgccga gccgtattac gacatctcgc gcgtggaaat
    13981 attgcgcggg ccgcagggca ccttcgtggg ccagaacgcc accggcggcg cggtcttcgt
    14041 cacgtcgcag gatcccgtca tcaatggagg tcacgatggt tacttgcagg cgcaagcggg
    14101 taactacaac tcgttcggcg cacagggcgc gatcaatcta ccggccggcg acaccttcgc
    14161 cgcgcgcatc gcattcaacg gtgatacccg cgacagtgtc tacgacatct cgggcccgta
    14221 cacgggtgac gagggcgtca agaccggcag cattcggctc ggcatgttgt gggagcccag
    14281 cgagaaactg tcgattctct ggaagaccga ctacaactat ctggatcttt ccggctatcc
    14341 cgccgacccg gtgaacgcca ccaacggaat gttcgatatc acggccaatg ccgagatcat
    14401 gggacgcgat cgcttcggcc gatcggtgct caagatcgac tacgaattcg gcaacggcac
    14461 gtcgctgcgt tccgtcacgg gttaccagga aggccaaacg gaataccgcg cggatctcga
    14521 cggcaccagc accggaaaca acatcttcgg cgatgcggtc gacgagacca ttttctcgca
    14581 ggagatcaac ctcatctcgt ccgatgcggg aaagttccag tggatcctcg gcgcgtatta
    14641 ccagcacgat gaatacgaat ttccgcccgg cgagttctac atcggcattc ccgccggctt
    14701 ctacctgctg gatggcacga atcccaagga aacttccgcc ctgttcggac aggtcagctt
    14761 cggtcttgcg gagggctggg aattggagat cggcgcgcgc tactcggaaa gcagcacgtc
    14821 caacgacatc aacgtcaatc aatacgggct tccgctcacc caggaccagg aggcggaatt
    14881 cacgaatctc tccggcaagg tttcgctgaa ctgggaagtc aatccgcacc acttcg
//
LOCUS       DASQJS010000243         3424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4221546, whole genome shotgun sequence.
ACCESSION   DASQJS010000243 DASQJS010000000
VERSION     DASQJS010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4221546"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..559)
                     /locus_tag="VEW08_09870"
     CDS             complement(362..559)
                     /locus_tag="VEW08_09870"
                     /inference="COORDINATES: protein motif:HMM:NF025156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HYJ41088.1"
                     /translation="MTKIEYKTVLLPYKTSLFQGDSDDLQTLLNKEGAEGWLLSQIVL
                     PSTVWGRANTMIAIMQRTAAA"
     gene            676..1953
                     /locus_tag="VEW08_09875"
     CDS             676..1953
                     /locus_tag="VEW08_09875"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HYJ41089.1"
                     /translation="MRHTKIAAIAALIGLALSSLTAMAGDSQNGHGHGDSYDRDHGRA
                     DQSIQLGPRPFYLVQGMDAGPLKNKLLRCQNGPFRRTDFSIAHRGAPLQFPEHTKESY
                     EAGARMGAGIVECDVTFTKDGQLVCRHDECDLHTTTNIVDTPLNASCTTPWSGANSGP
                     RCCASDITLAQFKTLKGKMDASNPAATTAAGYLGGTASWRTDLYTGRGTLLTLKESIA
                     LNRSLGVKHTPELKAGNPTRVNQVFGSQANYAQAMINEFKRAGVKPRDVFAQSFNLAD
                     VTHWVTHEPAFGKQAVYLDDVDPGAGIPPLSAAELVAVRNHGVRIFAPPIPALLAVDA
                     STNTLVPSQYAKDIKAAGLGIITWSFERADLRRGAAFGGFYAAYDPEGRVVKKDSDMY
                     YALDTLAQDVGILGIFSDWPATVSYYASCMGLE"
     gene            complement(2011..2982)
                     /locus_tag="VEW08_09880"
     CDS             complement(2011..2982)
                     /locus_tag="VEW08_09880"
                     /inference="COORDINATES: protein motif:HMM:NF023812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3658 domain-containing protein"
                     /protein_id="HYJ41090.1"
                     /translation="MTILVLLPGLDGTGDLFAPFVAALTGVDSRVIAYPADRAMDYPE
                     HEGFVRDKLPRDEDYVLLAESFSGPIGISIASTAPPGLRGLILCGTFASNPLPWLGPL
                     ANAIGRLPAMRMPPALAAPWLYAGRATPELRRAHASAMARVSAVTLRARVVAALGVDY
                     RSLVSRIEVPMMYLRAKSDRLIPAAAGQAIVDLRPETTMVVIDAPHFLLQTEPEVCAR
                     AVEKFLEHEERPDPPLDVEQSLRVSRLTQEDLWDIDREILSQSARSWRKVARVVIQVM
                     DKLAMRIPDLPDVYIAQRVRHLVEIGKLESQGNLHYMRYSEVRLPGQ"
     gene            3026..>3424
                     /locus_tag="VEW08_09885"
     CDS             3026..>3424
                     /locus_tag="VEW08_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ41091.1"
                     /translation="MKILVTGSTGHLGEALMRKLASGPHEAIGIDILRSPFTSIVGSI
                     ADRAVVDACMKGVDVVLHAATLHKPHVATHSRQDFVDTNVTGTLNLLEAAVANRVRSF
                     VFTSTTSTFGDALTPPRGAPAVWITESVVPI"
BASE COUNT          660 a         1105 c         1070 g          589 t
ORIGIN      
        1 aaatcggtgc ctggcaccat tctaaagcgc cagacggcac caatctcggc ggggtagtcc
       61 cagtgcgcct gtactccggt gtgggcgctg tccagcgcta acaagctgac gtgcggatcg
      121 aagttgtagt cgagttccgc gccaccggtg tagtcgtggc gatacacctc atcgaaggtc
      181 agggctgggt agtgtgtggc gccgcggagg cggccgcgac acaggcggcg gcaaacaaac
      241 tggaagcgga gcggggtttc atagccaact cctcgtgatg aaggagttgg ctagacttcg
      301 ggttcgcggc gcaagttgcg ttaagaatcg cgcggcgcgt tgagaatccg taaggcggcc
      361 gtcacgcagc agcggtgcgc tgcatgatcg cgatcatggt gttcgcgcgg ccccacaccg
      421 tggagggcag cacgatctgc gacagcaacc acccttccgc gccttccttg ttgagaaggg
      481 tctggaggtc atccgagtcg ccctggaaga gactggtctt gtaaggcagc aggacggtct
      541 tgtattcgat tttggtcatg ctccatcgtc gcacgcgcga gacactcgcg taaccaaaac
      601 tgtaaagcga tgttcacgaa tgtgacatcg ccccaccgaa tcatggcggc tcgtgacaca
      661 actccgggag aagccatgcg ccacacaaag attgctgcga tcgctgcgtt gatcggtctc
      721 gcgttgtcga gtctgaccgc gatggcgggc gactctcaaa atggccatgg ccatggcgat
      781 agctacgacc gcgaccacgg tcgcgccgat caatccatcc agcttgggcc ccgcccgttc
      841 tacctggtgc agggtatgga cgccggtccg ctgaagaaca agctgctcag gtgccagaac
      901 ggaccgttcc gtcgcaccga cttctccatt gcacatcgtg gcgcgccgct gcaatttccc
      961 gaacacacga aagagtccta cgaggccggc gcgcgcatgg gcgcgggcat cgtcgaatgc
     1021 gacgtgacat tcacgaagga cggccagctc gtgtgccgcc atgacgagtg cgatctgcac
     1081 accacgacca acatcgtgga cacgccgctg aatgcaagct gtaccacgcc gtggtccggt
     1141 gcgaactccg gcccccgctg ctgcgccagc gacatcacgc tcgcgcagtt caagacgctc
     1201 aagggcaaga tggatgcgtc gaatcccgca gcgactaccg cagccggata cctcggcggt
     1261 acggcgagct ggcgcacgga cctgtacacc ggccgcggca cgctgctcac gctgaaggag
     1321 agcatcgcgc tcaatcgcag cctcggcgtc aaacacacgc cggaactcaa ggctggcaat
     1381 ccgacgcggg tcaaccaggt gttcggcagc caggctaact acgcccaggc gatgatcaac
     1441 gagttcaagc gcgccggcgt caagccgcgc gatgtcttcg cacagtcctt caatctcgcg
     1501 gatgtcacgc actgggtcac gcacgagccc gcgttcggga aacaagccgt ctatctcgat
     1561 gacgtcgatc caggcgctgg cattccgccc ctatctgctg ccgaactggt tgccgtgcgc
     1621 aaccacggcg tgcgcatctt cgcgccgccg atcccggcat tgctggcggt ggatgcgagc
     1681 accaatacgc tcgtgccttc gcagtacgcg aaagacatca aggccgcggg cctcggcatc
     1741 atcacctgga gcttcgaacg cgccgacctg cgtcgcggtg cggccttcgg tggcttctat
     1801 gccgcgtacg acccggaagg ccgcgtggtg aagaaggaca gcgacatgta ctacgcgctg
     1861 gacacgctcg cgcaggatgt cggcatcctg ggcatcttct ccgactggcc ggccaccgtg
     1921 agctactacg cgagctgcat gggactggaa tagtttgatc aggccgcggg cgggcgccat
     1981 gcccccggcg ctcgcccgcg gaaggtgcgg ctactggcca ggtagccgca cctcgctgta
     2041 gcgcatgtag tgcagatttc cctgggactc gagcttgccg atctcgacga ggtggcgcac
     2101 gcgttgcgcg atgtaaacgt ctggcagatc aggtatccgc atggcgagtt tgtccatcac
     2161 ctggatcacg acgcgggcca cttttcgcca gctgcgagcg gactgcgaga gaatttcgcg
     2221 atcgatatcc cacaagtctt cctgcgtgag tctcgatacg cgcagcgact gctcaacatc
     2281 gagtggcggg tccggccttt cttcatgctc caagaacttc tccactgccc gcgcgcagac
     2341 ctcgggttcg gtttgtagca gaaagtgagg ggcgtcgatc acgaccatcg tcgtctccgg
     2401 acgcagatcg acgattgcct gtcccgcggc cgccggaatc aggcggtccg atttcgccct
     2461 caagtacatc atcggcactt cgatacgcga taccagcgac cggtagtcga cccccagtgc
     2521 cgctaccacc cgcgcgcgca gcgtaacagc cgacactctc gccattgccg acgcatgcgc
     2581 acggcgcaat tcaggtgtcg cacgaccggc gtacagccag ggagccgcca atgccggcgg
     2641 catgcgcatc gccggaagcc tgccgatcgc gtttgccagc ggaccgagcc atggcagcgg
     2701 attcgaagca aacgtgccgc agagaatcag gccccgcagt ccaggcggcg cggtcgacgc
     2761 gatcgagatg ccgatcggcc ccgaaaacga ttcggccaac agaacatagt cttcgtcgcg
     2821 cggtagtttg tcgcgcacga agccttcgtg ctccgggtag tccatggcgc gatccgctgg
     2881 atacgcgatg acccgcgaat cgacgcccgt caacgccgcg acgaatggag cgaacaggtc
     2941 cccggtgccg tcgagcccgg ggagcagaac caggattgtc atcggcctgc atgatgcgcc
     3001 agccgaaggt agactgcgtc gtcccatgaa gatattggtc accggcagca caggtcacct
     3061 cggcgaagcg ttgatgcgca agctcgccag cgggccgcac gaagccattg gcatcgacat
     3121 cctgcgctcg ccattcacga gcatcgtggg ttccatcgcg gaccgcgccg tggtcgatgc
     3181 ctgcatgaaa ggcgtggacg tggtgctgca tgccgccacg ctgcacaagc cgcacgtggc
     3241 cacgcacagt cgccaggact tcgtggacac caacgtcacg ggcacgctca acctgctgga
     3301 ggccgcggtg gcgaaccgcg ttcgctcgtt cgtgttcacc agcaccacga gcacgtttgg
     3361 cgacgcgctc acaccgccgc gcggtgcacc ggcggtgtgg attaccgaaa gcgtggtgcc
     3421 gatc
//
LOCUS       DASQJS010000244         9875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2968661, whole genome shotgun sequence.
ACCESSION   DASQJS010000244 DASQJS010000000
VERSION     DASQJS010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9875
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2968661"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1849
                     /locus_tag="VEW08_09890"
     CDS             <1..1849
                     /locus_tag="VEW08_09890"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HYJ41092.1"
                     /translation="LHVLSIDRKQRRRNPSPPFTTSTLQQEAARKLGYSAQRTMRLAQ
                     QLYEGVDFGEGAVGLITYMRTDSLSLAVDAVREMREVIAKEYGVEAVAEEPRIYKTKS
                     RNAQEAHEAIRPTSAAILPSQLEGKVDPDQYKLYALIWKRAVASQMAHALFDTVAVDM
                     LPSTYSEKTGAGAANGAGRHVMRANGSTMIKPGYMAVYQEDVDDGKPENENDRILPAM
                     KEGDVVTLLKVHGDQHFTEPPPRFSEASLVKALEEHGIGRPSTYATIISTLKDREYVD
                     MDNRRFIPTDIGKIVGSFLSKHFHKYVEYGFTASLEDELDAVSRGEEDWTEPLKKFWK
                     PFITQVEHIEKNVSREEVAQARELGKHPESGKPITVRMGRYGPFVQVGTKDDEEKPKF
                     AGLRPGQKMDLIKLPEALELFKLPITLGETAEGETVQANIGRFGPYIKFGAKYVSLKP
                     PDDPYTVDLPRALEVIRLKKEADANRIIQDFGVDDIQVLNGRYGPYITDGKRNARMPK
                     DTDPKAITLEVCRELLSVAPLRGTRFGRKKPAAKSKDAATPAVETTPKAKPAKKSAAK
                     SAQKSEAADTVPMKTGKKSTPARKKSARASHAKPVVAVPKKTSSKRKKT"
     gene            2067..5336
                     /locus_tag="VEW08_09895"
     CDS             2067..5336
                     /locus_tag="VEW08_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYJ41093.1"
                     /translation="MNSKRVGHGHVKWAVCAALLSATTLAAAAEPRALRDGSSRISSL
                     ALKAPVVMEARPRSVLHPSLQGLTGRQQVLVRLRGESVAGSDESVTREQVLAEQAAFI
                     NRVLGVVPSAEVISSTQLAINAVVLEVDAADLPALARDTAITRVVEVGDYRLDLSETV
                     PYIGADTAHDFGARGQGVRIAVIDSGVDYTHKALGGPGTQAAYEAAWAPLPPAGAPAI
                     PVVAAGTGYLVANDPATAADDGLFPGSKVIGGYDYVGERWTGGANSPPLAPDPDPIGA
                     PDATTNGGHGTHVADIIAGKLGVAPGAKLYAIKACSAPGTSCSGIALLDAMDFALDPN
                     GDGRIRDRVDIINMSLGSDYGQPFDDDLSAAVDNASKVGVLTVASAGNGSDKQFITGS
                     PAAASSALSVAQTEVPSSTLQLMTLIAPVTGDRAAVHQPWSGALGSIIEGPVTVFPAG
                     TTARTGCTTIAPAGPIPPWPAGSLNGKIVFVDRGACNFSEKIQNIQAAGGILGIIGLV
                     TPEAPFAGAFGVGAPPTIPGYMINQADGDILRGGNAVVRFDPLKQPSLAGSLASTSSR
                     GPRFDDNIVKPEIGAPGASISAGSGSFTGTSAFGGTSGAAPMVTGAAAVLKAARPFLK
                     PREIKQLLINTAETNVYQPSSAASVLPDQLAPITRIGGGEVRVDRALFSPVIVSDVTG
                     DAVSRVHGAMSFGYLDASKPSTTLTRKLEVENHSFFPLVYKVKSTLRHQDDKDTGAVS
                     ISVSPSTIYVGPFGSAKVTVKLNVDGTRLRPNLMNSSTAGNNSGPLTANEYDGYIVFD
                     GLHHKVTMPWHILPRKSADVVAKLPGGHLPINSAGLGSVSVENKGVGDAQMFAYSMLG
                     TGTNRPGGAHGENSPTPDIRAVGVNTFGVPAGFCAATENFIWEFAFNMFERPTMPIGT
                     WHEVDLDVDGDGGDDFQIYTRDVSGDFGLEDGRQATAVYDVNAGTVLLRSDSFTMEHA
                     TNSSTLVMRICGSDLGMTLADIGRPVSADFRAISWYWGTPQSHLGPFKIAPGGEEFSA
                     GIPGAVLGYKQKGSLDVRQWGLFPGTDPHAGLLLINNSAFSDTNNGGATAASEAILLP
                     R"
     gene            complement(5417..5878)
                     /locus_tag="VEW08_09900"
     CDS             complement(5417..5878)
                     /locus_tag="VEW08_09900"
                     /inference="COORDINATES: protein motif:HMM:NF018430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HYJ41094.1"
                     /translation="MRFTVRGVPQEIADEVRRTHLSPGYGHPVHLEIASGTGPCRCCL
                     RPFVAGRDQRLLFTFRPRSDDGSLMAPGPVFIHAGHCEAFSGAGFPEALRTLPLAFEA
                     RKSGSRVSELSARPDVSPEAQIDVLFREHEARWLHVRHAEAGCFIARVDRG"
     gene            complement(5952..6503)
                     /locus_tag="VEW08_09905"
     CDS             complement(5952..6503)
                     /locus_tag="VEW08_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41095.1"
                     /translation="MKPRRLALRVYTLFSQEPMMSKSLKILILAGVFAASAACAADTP
                     LTPRQIAPPKRIPDIETLTPLTAGEPVSTALIPREVRRAVVADAAKRFNVAESAVVLT
                     RAEQLTWSDGSLGCPEPGVMYTQMLVAGFRVVAKTSAGELTYHTDSRGNAVSCNARAR
                     RGDTGDDASAPHTLPPPRSPPDR"
     gene            6502..7320
                     /gene="rlmJ"
                     /locus_tag="VEW08_09910"
     CDS             6502..7320
                     /gene="rlmJ"
                     /locus_tag="VEW08_09910"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HYJ41096.1"
                     /translation="MKYRHQYHAGNFADVHKHVLLLEVLRALTRKDKGLLFVDTHAGR
                     GDYDLHPGDREHPAEWQAGVARLLAAAPRHAALVSYREIIERGMHGGSLHYPGSPLLA
                     MRALRPVDRAVFFETQREEAGHLRQALEEHGRARVEVADGFAGLRALLPPPERRGCVL
                     IDPPYEEREDLTRVQQALQDALQRFESAVLVAWLPVKLREDFDAWYGQLGRAVNRPML
                     TSLLWMHPCDSRAALNGSALVVVNPPYLVEGAMREWLPEAQTILGGPQSGCEVL"
     gene            complement(7322..7990)
                     /locus_tag="VEW08_09915"
     CDS             complement(7322..7990)
                     /locus_tag="VEW08_09915"
                     /inference="COORDINATES: protein motif:HMM:NF023287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3379 family protein"
                     /protein_id="HYJ41097.1"
                     /translation="MSGVFECKHARLAIGGDPQALSAEVAQHIATCPQCARFRDETLA
                     MEERLKAALELPLHRFRKPTETSKAAPRRFALAASLVLALLVGGGAWLFRPQPALAAE
                     VIEHVNHEPGSWQKHEPVSAEALAAVLAKAGVRFDARFPVTYASPCPFRGHIVPHLVV
                     QTDRGPLTVMLLAHVTAEGAGKFSEGEYHGVVLPAGPGSIAVVAREGQEFDGYLKPLL
                     EGVR"
     gene            complement(7987..8523)
                     /locus_tag="VEW08_09920"
     CDS             complement(7987..8523)
                     /locus_tag="VEW08_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ41098.1"
                     /translation="MNPRPRGNTATSAFARLLETLRPDLVRFAFWLARDRAVAEDVVQ
                     ETLIRAWKSREEIKDPAATRPWLLTIVRREHARLYERKRLPTVDLADVEVQGDPALLS
                     SDTSDGDAGLDDLRRAILQLPDEYREPLVLQVLGGLTTAEIGEQLGLTQAAVLTRLFR
                     ARNRLRVIYGLTTAEDVE"
     gene            8587..9411
                     /gene="aroE"
                     /locus_tag="VEW08_09925"
     CDS             8587..9411
                     /gene="aroE"
                     /locus_tag="VEW08_09925"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HYJ41099.1"
                     /translation="MNAPDQYGVVGHPINHSWSPFIHGLFAKQTEQSLVYRLYDIAPE
                     DFRAQVLEFFTRGGRGLNVTVPHKEAAAELANELTVRADRAGAVNTLMVNSEHNLLGD
                     NTDGYGLVTDLRKNLGLTITNDRILILGAGGATRGVVEPLLELGPTELVVANRSPDRA
                     VNLASLFVDLGAIRGCGFEDVGDEPFDLVINATAAGLTGAVPNVDGSVIASHTVCYDM
                     SYSKASTPFVSWSIEQGCARAHKGWGMLVEQAAEAFTLWRGVRPDTEPVLAVLGTK"
     gene            9408..>9875
                     /locus_tag="VEW08_09930"
     CDS             9408..>9875
                     /locus_tag="VEW08_09930"
                     /inference="COORDINATES: protein motif:HMM:NF040252.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41100.1"
                     /translation="MTDANIRGTTLPAFDRIAASEVEPTIRELLQRNRGEVATLESQP
                     HPTFANTVLPLEQLSHRLSRTWSPISHLNGVLNSDELRASYNACLPLLSNYWTDLAQS
                     EPLFRAYTAIAANESGSLDAAQRRVIERALEDFRLAGVGLPAERKARYKAVVQE"
BASE COUNT         1805 a         3224 c         3155 g         1691 t
ORIGIN      
        1 cctgcacgtg ttgtccatcg accgcaagca gcgccgccgc aatccgtcgc cgcctttcac
       61 cacctccacg ttgcagcagg aagccgcgcg caagctcggc tattccgcgc aacgcacgat
      121 gcgcctggcc cagcagcttt atgaaggtgt cgacttcggc gaaggcgctg tcggtctcat
      181 cacctacatg cgcacggatt cgctgagcct ggccgtcgac gccgtgcgcg agatgcgcga
      241 ggtcatcgcc aaagagtacg gcgtagaggc ggtcgccgaa gagccgcgca tctacaagac
      301 caagtcacgc aacgcgcagg aagcgcacga agccattcgc ccgacgtccg cggccatcct
      361 gccctcgcag ctcgagggca aggtcgatcc ggatcaatac aagctgtacg ccctcatctg
      421 gaagcgcgcg gtcgcgtcgc agatggccca cgcgctgttc gacacggtcg cggtcgacat
      481 gctgccgagc acttattcag aaaagacggg ggctggggct gcgaacggcg ccggccgcca
      541 tgtcatgcgc gcgaatggtt cgaccatgat caaacccggc tatatggccg tctatcagga
      601 agacgtcgac gacggcaaac ctgagaacga aaacgaccgc atcctgcccg ccatgaaaga
      661 gggcgacgtc gtcacactgc tcaaggtgca tggcgaccag cacttcaccg aaccgcctcc
      721 ccgattctcg gaagcgtctc tcgtgaaagc tctcgaggag cacggcatag ggcgcccctc
      781 gacatacgcc accatcattt cgacgctcaa agatcgtgaa tacgtcgaca tggacaaccg
      841 gcggttcatt ccgacggaca tcggcaagat tgtcggcagc ttcctgtcca agcatttcca
      901 caaatacgtg gagtacggtt tcaccgcctc gctcgaagac gagctcgacg cggtgtcgcg
      961 cggcgaagaa gactggactg agccgctcaa gaaattctgg aagccgttca tcacccaggt
     1021 cgagcacatc gaaaagaatg tctcgcgcga agaagtcgcg caggcccgtg aactcggcaa
     1081 acacccggaa agcggcaagc ccatcaccgt gcgcatgggc cgttacggtc cgttcgtgca
     1141 ggtcggcacc aaggacgacg aagagaagcc gaagttcgcg gggctgcgtc ccggccagaa
     1201 gatggacctc atcaagctgc ccgaggcact cgagctcttc aaactaccga tcacgctcgg
     1261 cgagaccgcc gagggtgaga cggttcaggc caacatcggc cgcttcgggc cgtacatcaa
     1321 gttcggcgcg aagtatgtat cgctgaagcc gccagacgac ccgtacacgg tcgatctgcc
     1381 gcgcgcactc gaagtcatcc gcctcaagaa agaagcggac gcgaaccgga taatccagga
     1441 cttcggcgtc gatgacatcc aggtgttgaa cggccgctac ggcccgtaca tcaccgacgg
     1501 caagcgcaat gcgcgtatgc cgaaggacac ggacccgaag gccatcacgc tcgaggtgtg
     1561 ccgcgaattg ctttcggttg ctccgctgcg cggcacgcgc ttcggtcgca agaaacctgc
     1621 cgcaaagtcc aaagatgcag caactcccgc ggtggaaacc acgccgaagg cgaagcccgc
     1681 gaagaaatcc gcagccaaga gtgcacaaaa gtcggaagcg gctgacaccg tgccaatgaa
     1741 gacggggaaa aaatcgactc cggccaggaa gaaatcggcg cgcgcgagtc atgcgaagcc
     1801 ggtcgtggcc gttcccaaaa aaacgtcatc gaaacgtaaa aaaacctgat ccagttatgt
     1861 tcaatctcgc caggaactgg cgaagtcacg cgattgaatt aagtcgtaac gcggagtagc
     1921 gtcgccggcg ggtcctggat atcacggtgt gaagctcgaa ggggagattc gcactgctcg
     1981 cggcactaca gaagaaccgc cgcgctcccg agcacgagtg ctcggcgtat gtctggaata
     2041 acaggttctc agggaagaca ttcaaaatga atagcaaacg tgttggtcac ggccacgtga
     2101 agtgggccgt gtgcgccgct ttgttgtcgg caaccacgct cgcggccgct gccgagccgc
     2161 gcgctctacg cgatggcagt tcgcgtatct cctcgctcgc gctcaaagcg ccggtggtca
     2221 tggaagcccg ccctcgttcg gtgctgcatc cgagtctcca gggactcacg ggccggcaac
     2281 aggttctcgt gcgcttgcgc ggagaatcgg tggctggctc cgacgagtct gtcacccgcg
     2341 agcaggtgct cgccgaacaa gcggccttca tcaatcgtgt gctcggcgtc gtgccaagcg
     2401 ctgaagtcat ttcgtcgacg cagctcgcga tcaatgcggt ggtgctcgaa gtcgacgccg
     2461 cggatctgcc ggcgctggcg cgcgacacgg cgatcacgcg cgtcgtggaa gtcggtgact
     2521 accggctcga tctctcggaa accgtcccgt acatcggcgc ggataccgcg cacgacttcg
     2581 gcgcccgtgg ccagggtgtg cgcatcgccg tcatcgacag cggcgtcgat tacacgcaca
     2641 aggccctggg tggtcccggc acgcaggctg cttatgaagc cgcctgggcg ccgcttcctc
     2701 ccgcgggtgc accggcgatt cccgtggttg ccgcgggcac gggctatcta gtggccaatg
     2761 accccgcaac cgctgcggat gacggcctct ttcccggttc caaggtgatc ggtggttatg
     2821 actacgtcgg tgagaggtgg accggtggtg caaactctcc gccgctggcg ccagatccgg
     2881 atccgattgg cgctcccgat gcgaccacga acggtggcca cggcacgcat gtcgccgaca
     2941 tcatcgccgg caagctcggc gtggctccgg gcgcaaagct ctacgcgatc aaggcctgct
     3001 ccgcgccggg tacgtcgtgc agcggcatcg cgctgctcga tgcaatggac ttcgcactcg
     3061 acccgaatgg cgacggccgc atccgcgacc gcgtcgacat catcaacatg tcgctcggtt
     3121 cagactacgg tcagcccttc gatgacgacc tgtctgctgc ggtcgacaat gcttcgaagg
     3181 tgggcgtttt gaccgtcgcc tccgccggta acggctccga caagcagttc atcaccggtt
     3241 cgccggcggc tgcttcttcc gcgttgagcg tggctcagac cgaggtgccc tcgtccactt
     3301 tgcagctcat gaccctcatt gcaccggtca ccggcgaccg tgccgcggta caccagccgt
     3361 ggtccggcgc tctggggagc atcattgaag ggccggtcac cgttttcccc gcgggcacca
     3421 cggctcgaac cggctgcacg acgatcgccc cggccggccc gattccgccg tggccggcgg
     3481 gttcactgaa cggcaagatc gtgttcgtcg accgcggtgc ctgcaacttc tcggagaaga
     3541 tccagaacat ccaggcggct ggcggcatcc tcggcatcat cggcctggtc actccggagg
     3601 ctccctttgc gggcgcgttc ggtgtcggtg caccgccgac gattccgggc tacatgatca
     3661 accaggccga tggcgatatt ttgcgcggcg gcaatgccgt agtgcggttc gacccgctga
     3721 agcagccgtc gttggcgggc tcgctggcat cgacctcctc gcgtggtccg cgcttcgacg
     3781 acaacatcgt caagcccgaa atcggcgcgc cgggtgcgtc gatctccgcg ggttcgggca
     3841 gcttcaccgg gacctcggcc ttcggtggca cctctggtgc ggcgccgatg gtcacgggtg
     3901 cggcagccgt gctcaaggct gcgcgtccgt tcctgaagcc acgcgagatc aagcagctgc
     3961 tgatcaatac ggccgagacc aatgtgtacc agccctcgtc ggctgcgtcg gtgctgccgg
     4021 atcagctcgc gccgatcacg cgtatcggcg gcggtgaggt gcgcgtggat cgtgcgttgt
     4081 tctcgccggt gatcgtcagt gacgtgaccg gagatgcggt gtcacgtgtc cacggggcca
     4141 tgagcttcgg ttatctcgat gcctcaaagc cctcgacaac gctcacgcgc aagctcgagg
     4201 tggagaacca ttcgttcttc ccgctggtgt acaaggtgaa atcgacgctg cggcaccagg
     4261 acgacaagga caccggcgcc gtcagcatca gtgtcagccc gtccacgatc tatgtcgggc
     4321 cgtttggttc ggccaaggtc acggtgaagc tcaacgtcga tggcaccagg ctgcgcccca
     4381 acctgatgaa ctcgagcacg gccggcaaca atagcgggcc attgaccgcg aatgaatacg
     4441 acggctacat cgtgttcgat ggtcttcacc acaaggtgac catgccgtgg cacatcctgc
     4501 cgcgtaagtc ggccgacgtg gtggccaagc tgccaggcgg ccacctgccg atcaattcag
     4561 cgggtctcgg atcggtcagc gtcgagaaca agggcgttgg agatgcgcag atgttcgcgt
     4621 actccatgct cggcaccgga acgaatcgtc cgggtggcgc gcatggcgaa aattccccga
     4681 ccccggacat ccgcgccgtg ggcgtcaata cgttcggagt cccggccggg ttctgcgccg
     4741 cgacggagaa cttcatctgg gagttcgcgt tcaacatgtt cgaacgtccg acgatgccga
     4801 taggaacctg gcacgaagtc gatctggacg tggatggcga cggaggagac gacttccaga
     4861 tctacacgcg cgatgtctcg ggtgacttcg gcctcgagga cggccgccag gcaaccgcgg
     4921 tgtacgacgt gaatgcggga acggtgttgt tgcgctccga ctctttcacg atggagcacg
     4981 ccacgaactc gtccacgctc gtcatgcgta tctgcggcag cgatctcggc atgacgctgg
     5041 cggatatcgg gcgaccggtg agcgccgatt tccgcgcaat ctcctggtac tggggcacgc
     5101 cacaatcgca ccttgggccg ttcaagatcg ctccaggcgg cgaggaattc agcgcaggga
     5161 ttcctggcgc tgtgctcggt tacaaacaaa aaggttcgct cgatgtgagg cagtggggtc
     5221 tcttcccggg tacggacccg cacgccggtc tcctgctcat caacaacagc gccttctccg
     5281 atacgaacaa cggtggtgcc actgcggcgt cggaggccat cctgctgccg aggtgagtgc
     5341 cggttacgac agctgacgcc gtctttcgag acggcaaccc atcagccccc gcgggaaacc
     5401 gcgggggctt ttttatttac ccgcggtcga cgcgtgcgat gaaacatccg gcttcggcat
     5461 gacgcacgtg caaccagcgc gcctcgtgct cgcggaacag cacgtctatc tgcgcctcgg
     5521 gggacacgtc ggggcgcgcg gacagctcgc tcacgcgact gcctgatttt cgcgcttcga
     5581 aggcgagcgg cagcgtgcgc agcgcctcgg gaaaacctgc tcccgagaac gcctcgcagt
     5641 gtccggcatg gatgaacacc gggcccggag ccatgaggct tccgtcatcg ctgcgcggtc
     5701 ggaaggtaaa gagcaggcgc tgatcgcggc cggcgacgaa cggcctgagg caacaacggc
     5761 atgggccggt gccgctcgcg atctcgagat ggacgggatg cccgtatccc ggagaaagat
     5821 gggtgcgccg cacttcgtcg gcaatttcct gtgggacacc acgtacggtg aatcgcatgt
     5881 cttgctcccg gtgacgtggc gaaagtctgc cgcgccatgg agcaaccagc gacccgatgc
     5941 ttgcgctccg ttcagcgatc cggcggcgat cggggaggag gcagcgtatg cggtgcggaa
     6001 gcgtcgtcgc ccgtgtctcc gcgccgggcc ctcgcgttgc agctcacggc attgccgcgc
     6061 gaatcggtgt gataggtgag ctcgccggcg ctcgtctttg cgaccacgcg gaatcccgcc
     6121 accagcatct gggtgtacat gaccccgggt tcggggcagc ccagggaacc gtcgctccag
     6181 gtcagttgct cggcgcgcgt gagcaccacc gcgctttcgg cgacattgaa gcgctttgcg
     6241 gcgtctgcaa ccaccgcgcg ccgcacctcg cgcgggatca gcgcggtgga taccggctca
     6301 ccggcggtca agggcgtcaa cgtttcgata tccggaatgc gcttcggtgg agcgatctgg
     6361 cgcggagtca gcggtgtgtc tgcagcgcag gcggccgacg ccgcgaacac tccggcaagg
     6421 atcagtattt tcagggactt cgacatcatc ggctcctggc tgaacagtgt ataaacgcgc
     6481 agcgcgaggc gccggggttt catgaagtac cgccatcagt atcacgccgg caattttgcc
     6541 gacgtccaca agcacgtctt gctgctcgag gtcctgcgcg cgctgacgcg caaggacaag
     6601 ggcctgctgt tcgtcgacac ccacgcgggg cgcggcgact acgatcttca tcccggcgat
     6661 cgcgaacatc cggccgagtg gcaggcgggt gtcgcgcgcc tgctcgcggc ggcgccgcgt
     6721 cacgcggcgc tcgtgagtta tcgcgaaatc atcgagcgcg gcatgcatgg cggcagcctg
     6781 cattatccag ggtcgccgct gctcgcaatg cgggctctgc ggcccgtgga tcgcgccgta
     6841 tttttcgaga cgcagcgcga ggaagccggc catctacgcc aggcgctgga ggaacatgga
     6901 cgcgcgcgcg tcgaagtcgc cgacggcttc gccgggttgc gcgcgctgct gccgccgccc
     6961 gagcgacgcg gttgcgtgct catcgatcca ccctacgagg aacgcgagga tctgacccgt
     7021 gtgcagcaag cgctacagga cgcgctgcag cgcttcgaaa gcgcggtgct ggtggcctgg
     7081 ctgccggtga aactgcgcga ggacttcgac gcgtggtatg gccagctggg gcgcgcggtc
     7141 aaccgtccga tgctgacgtc gctgctctgg atgcatccct gcgactcgcg cgccgcgctc
     7201 aacggatcgg ccctggtggt ggtgaacccg ccctatctag tcgaaggggc gatgcgcgaa
     7261 tggctgcccg aggcgcagac cattctcggg ggaccgcagt cgggctgcga ggtgttgtga
     7321 atcagcgaac gccttccagc aggggcttga gatagccgtc gaattcctgg ccctcccgtg
     7381 cgaccacggc gatgctgccc gggccggcgg gcaacacgac gccgtgatat tccccttcgg
     7441 aaaatttgcc ggctccttca gccgtcacgt gcgccagcaa catcacggtc aacggaccgc
     7501 ggtcggtttg caccacgagg tgcggcacga tgtgcccgcg gaaaggacaa ggcgaggcgt
     7561 aggtcaccgg gaagcgcgcg tcaaaacgta ccccggcttt cgcgagcact gccgcgagtg
     7621 cttccgccga caccggctcg tgtttctgcc agctgcctgg ttcatggttc acgtgctcga
     7681 tgacctcggc tgccagtgcc ggctgcggac ggaagagcca ggcgccgcct cccaccagca
     7741 gggcaagcac caatgacgcg gccagcgcga atcgccgcgg cgcagccttg ctggtctccg
     7801 tgggtttgcg gaagcgatgc agcggcagct cgagtgcggc cttgagccgt tcttccatgg
     7861 ccagcgtttc gtcacggaaa cgcgcgcatt gcgggcaggt ggcgatgtgc tgcgccacct
     7921 cggcggacaa cgcctggggg tcgccgccga tggccaggcg cgcatgtttg cattcgaaca
     7981 cgccgctcat tcgacatcct ccgcagtcgt gagtccgtag atcacgcgca gccggttgcg
     8041 tgcgcggaac aggcgggtca gcaccgcggc ctgcgtgagg cccagctgct cgccgatttc
     8101 cgctgtggtc aggccgccca gcacctgcag gaccaggggc tcacgatatt cgtcgggcag
     8161 ctgcaggatg gcgcggcgca ggtcgtccag gcctgcatcc ccatcactgg tatcgctgct
     8221 caacagggcc ggatcaccct ggacctcgac atcggcgaga tccacggtag gcagacgctt
     8281 gcgttcatag agacgggcat gttcgcggcg cacgatggtg agcagccacg ggcgcgtggc
     8341 cgcgggatcc ttgatctcct cgcgggactt ccaggctctg atgagtgtct cctgcaccac
     8401 gtcctccgcc acggcccggt cgcgtgccag ccagaaggcg aaacgcacca ggtccgggcg
     8461 caaggtttcc agcaagcgcg cgaacgccga agtcgccgtg tttccgcgtg ggcgtgggtt
     8521 catgtataag accggccgag tgaagcgctt atttcagcca tcttcctggt cagagttgcc
     8581 ttggcaatga acgccccgga tcagtacgga gtggtcggtc atccgatcaa tcacagctgg
     8641 tcgcctttca tccacgggct ctttgcgaaa cagaccgagc agtccctggt ttaccggctc
     8701 tacgacatcg ccccggagga ctttcgcgcg caggtactgg aattcttcac acgcggcggc
     8761 cgcggcctga acgtgaccgt accacacaag gaagcggcgg ccgaactggc caacgagctc
     8821 accgtccgcg ccgaccgcgc cggcgccgtc aacaccctca tggtcaactc cgagcacaac
     8881 ctgctcggcg acaacaccga cggctacggg ctggtcaccg accttcgcaa gaacctcggc
     8941 ctcaccatca ccaacgaccg gatcctgatt ctcggcgctg gcggcgcgac gcgcggcgtg
     9001 gtcgaacccc tgctggagct cgggcccacg gagctggtcg ttgcgaaccg ctcaccggac
     9061 cgggcggtca acctggcttc gttgttcgtc gacctcggcg cgattcgcgg ctgcggattc
     9121 gaggacgtcg gcgacgaacc cttcgatctc gtcatcaacg ccaccgccgc cggcctcacg
     9181 ggcgccgtgc cgaacgtgga tggctctgtc atcgccagcc acacggtctg ctacgacatg
     9241 agctacagca aggcgtcgac gcctttcgtg agctggtcca ttgaacaagg ctgcgcccga
     9301 gcccataaag gctggggcat gctcgtcgag caggccgctg aagcgttcac gttgtggcgc
     9361 ggcgtacgac ccgacaccga gcccgtgctg gccgttcttg gaaccaaatg accgacgcaa
     9421 acatccgcgg gaccacgctg cccgccttcg accgcatcgc cgcctcggaa gtggagccca
     9481 ccatccgcga gttgctgcag cgcaatcgtg gtgaagttgc gacgctcgaa tcccagccgc
     9541 acccgacctt cgccaacacc gtgttgccgc tcgagcagct ctcgcatcgc ctgtcgcgca
     9601 cctggtcgcc catcagccac ctgaacggcg tgctcaattc cgacgaactg cgcgccagct
     9661 acaacgcctg cctgccgctg ctgtctaact actggaccga cctggcgcag agcgagccgc
     9721 tgttccgcgc ctatacggcg atcgccgcga acgaatcagg ctcgctcgac gccgcccagc
     9781 gccgcgtcat cgaacgcgcg ctcgaagatt tccggctcgc cggcgtgggg cttccggcgg
     9841 aacgcaaggc cagatacaag gccgtggtgc aggag
//
LOCUS       DASQJS010000245         6685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2027194, whole genome shotgun sequence.
ACCESSION   DASQJS010000245 DASQJS010000000
VERSION     DASQJS010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027194"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..2801)
                     /locus_tag="VEW08_09935"
     CDS             complement(108..2801)
                     /locus_tag="VEW08_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ41101.1"
                     /translation="MLFMVAGWGGEAVTDYIGAWGSFPVSVGICVVLWPSLFDAALSQ
                     RRRRAWRLIFAALVLDLIASVGWGYTALTENVTFGSWPDVLYIFYYPLAAWAFVLLYL
                     DLGGRARTMRAWIDFTTVAIGFGSLLWFTALAPVAAMSGDDIMETWSVVGYGIGDAIA
                     IIAGAMVAMQISNWREERPIVWLLIAMVVTLVSDLLWVNAELRGTYVIGGASDVGYFV
                     FYVCMFAAVRSQLGHRQPVASTLGLQGDLRGSLPIVALMVGTIALLDDRLQLASGQSP
                     LLIWVLVAATLLVVAGQAFSTREVVSLNREVATRRFDERLTELVRRSSDMIAICDPDG
                     VIRYASPSSEQLLGVAPGELAGQRLDAVLGPESPHLREVFDQVVRAAHSEQTANFSIP
                     QGDDEKRSYKMVIANLCHVESIRGVTLNIRDISDATRLHDQLRTLAFHDSLTLLANRS
                     LFADRVHQAIRHIADGRTPAVLFIDLDNFKTVNDSLGHSAGDALLRAFAHRLVQSTRA
                     GDTVARLGGDEFAVLIDHAPNIEAAMAVARQVLDACRQPFEIDGRPLRVGCSVGVALA
                     DRVSNVERLLRNADAAMYSAKSNGKGHAEVFQPEMLRAARRRIRIENELTTAIEEQQF
                     EAHYQPIVDLTSRHLVGVEALMRWRHPTRGLVMPGDFIPLAEETGQIVAMTQWILNRA
                     CADLARLQRDVAHGEGIRVSVNVSSRYLNHGNIVEDVRNALATHQLAPNCLILEVTES
                     LLLENSESLERTFQELKRIGVRIALDDFGTGYSSLAYLHRFPIDIIKIDRSFVERLAG
                     DREGAGGVDAVALARAILSLAEALDLDTVAEGIEHDIQRDTLLELGCKTGQGYLFGKA
                     MPVAEVLDAAVTRRRSLLAGNVPGQIEYSATGRFRGIIESG"
     gene            3081..3800
                     /locus_tag="VEW08_09940"
     CDS             3081..3800
                     /locus_tag="VEW08_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HYJ41102.1"
                     /translation="MIAFTACGAVCAHVAHADAVQVVSGSIGGTSDFVFRGLSLTRGK
                     PAAQASIDVEFPKEFYIGGFVSTADPKSNPSPDLELDLWAGRYWRLSENFSGDLRLSQ
                     YTYPNDPRRVSYNRTELTATLGLHNRFFAAAIYSPNTSAIGSSAGYDEGNAWALELSG
                     RQPINQHLSISAGFGHYGLEEVYHDSYNYWNVTLIAAFKAFEFQFAYLGVDDSLEQHF
                     EPDSVGDRFAVTALWRFSTTK"
     gene            3867..4472
                     /locus_tag="VEW08_09945"
     CDS             3867..4472
                     /locus_tag="VEW08_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ41103.1"
                     /translation="MALKSVEFQSPRAPGASPDASERELLERIARSKDQAAFRTLYGS
                     YYQRLSRLLARMSVRREDIEEVINDTFWVVWTKAGEFRGASQLSTWIIGIAYRRALNA
                     LRRAKIRPVSDEPFDEDSISVMSTDAAETDGQWLALGLTRLPVEQRMALELTYTLGHS
                     CEEVAAILDCPVNTVKTRLFRARETLKQVLPALAGMSGEKS"
     gene            4469..5164
                     /locus_tag="VEW08_09950"
     CDS             4469..5164
                     /locus_tag="VEW08_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYJ41104.1"
                     /translation="MSARIPDTHAEAWALLPWLANGRLPASDREWVEAHVAHCAECRG
                     ELDAQRKVATQISRDTSPDMAGTPGSTGYGSTGNDEQRSFNKLWARIEASEGAAPSAS
                     GVAGGFSSRTSRTVRWLAAAVVVQGFGLALFGFNALRDNAGEIVTVAEVPAQRLNAPA
                     VRVVFAPEASMATINTLLTHQELSIVSGPGTSGNFTAELSAKAVASGASADSVAAVLS
                     KDPNVTFAQPVAR"
     gene            5133..6467
                     /locus_tag="VEW08_09955"
     CDS             5133..6467
                     /locus_tag="VEW08_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYJ41105.1"
                     /translation="MSLSPNRLRADTHMDWRLGVAVTLAALAFALSGGAIAAAPPVLT
                     PPEVRSDPAQFIVVAVANPVTGRPGAVGGTAHGYGSSTNYRVSASATAIIREITSRYS
                     LTRVSEWPIDELQMHCVLFRIPPGTTREAIIEKLKADRRILIVQPLNEFESATETPVA
                     APAVTFDDPYSRLQTNVRALDVAEAHNFSRGAGIRVAIIDTGVDTRHPDLVGRTHLTR
                     NYIDSDEVAFRGDRHGTQVAGLIAAAANNGVGIVGVAPDVRLMAYKACWQASASTTGR
                     CNSFTIAQALADALAAKAQVINLSLVGPSDPLLEALVAKAIAAGVIVVGAVSDDPRFA
                     FAAKLPSVLPVAESETSANQGDEILRAPARDIVTLVPNGHYDFASGSSLATAQISGVV
                     ALLLARNQKLGISRLRELLSQSTENHDTTRGPFRSVNACVAVARLVRGASCN"
BASE COUNT         1223 a         2195 c         2115 g         1152 t
ORIGIN      
        1 gcaggtctca gcttttcggg cactgacggc ccgggctgag caaggaccgg attatgagaa
       61 ccattccggg agttatgtaa tgcgccgggc tggcggcgta accggtgtca gccgctctcg
      121 atgatcccgc ggaaccggcc ggtggcggag tattcgatct ggccgggcac gttaccggcc
      181 agcaggcttc ggcggcgcgt caccgcggcg tccagcactt cggccaccgg catggccttg
      241 ccgaacagat atccctgccc ggtcttgcag ccgagctcca gcagcgtgtc gcgctgaatg
      301 tcgtgttcga tgccttccgc cacggtatcg aggtcgagcg cctcggccag cgaaaggatg
      361 gcgcgcgcca gcgcaaccgc gtcgactccg ccggcgccct cacggtcacc cgccaggcgc
      421 tcgacgaaag accggtcgat cttgatgatg tcgatgggaa agcgatgtag ataggcgagc
      481 gaggaatacc cggtgccgaa gtcatcgagc gcgatcctca cgccaatcct cttgagttcc
      541 tggaacgtgc gctcgaggct ttctgaattc tccagcaaca ggctctcggt cacctcgaga
      601 atgaggcagt tgggtgcgag ttgatgcgtg gcgagcgcgt tgcgcacgtc ttcgacgata
      661 ttgccgtggt tcagatagcg gctcgacacg ttgaccgaga cgcggatgcc ctcgccgtga
      721 gccacgtcgc gctgcaggcg cgccaggtcg gcgcaggcgc gattcaggat ccactgagtc
      781 atcgcgacga tctggcccgt ttcctcggcg agcggaatga agtcaccggg catgaccagt
      841 ccgcgcgtcg ggtgccgcca gcgcatcaat gcttcgactc cgaccaggtg ccgcgaagtc
      901 agatcgacga tcggctggta gtgcgcctcg aactgctgtt cttcgatggc cgtggtgagt
      961 tcattttcga tgcgaatgcg ccgccgcgcc gcgcgcagca tttcgggctg gaagacttcg
     1021 gcgtggccct tgccgttcga cttggcggag tacatcgccg cgtcggcatt gcgcagcagt
     1081 ctttccacgt tgctgacgcg atcggccagc gccacgccga cgctgcagcc gacgcgcaag
     1141 ggccgcccat cgatctcgaa aggctggcgg caggcatcga ggacctggcg tgcgaccgcc
     1201 atcgccgcct cgatattcgg tgcgtgatcg atgagtaccg cgaattcatc gccgcccagt
     1261 cgggccacgg tgtcgcccgc ccgcgtggac tggaccaggc gatgcgcgaa tgcgcgcagc
     1321 agcgcatcgc cggcgctgtg ccccaggctg tcgttcacgg tcttgaagtt gtcgaggtcg
     1381 atgaacaaca ccgccggcgt ccttccgtcg gcgatgtgcc tgatggcctg gtgcacgcga
     1441 tcggcgaaca gtgaacggtt ggcgagcagc gtcagggaat cgtggaacgc gagcgtgcgc
     1501 agctggtcgt gcaggcgcgt ggcatcggaa atgtcgcgga tattcagcgt cacgccgcga
     1561 atcgactcca cgtggcacag gttggcgatg accatcttgt aggaacgctt ctcgtcgtcg
     1621 ccctgcggaa tcgaaaaatt cgccgtctgt tcggaatgcg cggcgcgaac cacctggtcg
     1681 aatacctcgc gtaagtgcgg cgactccggc ccgagcactg catcgagacg ctggccggcg
     1741 agttcgccgg gcgccacgcc taacaactgc tcggacgagg ggctggcata acgaatgact
     1801 ccatcggggt cgcagatcgc gatcatgtcg ctcgagcggc gcaccagctc ggtcaggcgc
     1861 tcgtcgaagc ggcgtgtggc cacttcgcgg ttcaggctca ccacttcgcg cgtggagaaa
     1921 gcctggcccg cgaccaccag cagcgtcgcc gcgaccagca cccagatcag caacggcgac
     1981 tggccgctgg cgagctgcag acggtcgtcg agcaacgcga tggttccgac catgagagcg
     2041 acgatgggca gcgagccgcg cagatcgccc tgaagcccca gcgtcgacgc caccggctgg
     2101 cggtggccga gctgggaacg cacggcggcg aacatgcaga catagaacac gaagtagccg
     2161 acgtcgctcg cgccgccgat gacgtaagtg ccacgcagct cggcgttgac ccacagcagg
     2221 tcggagacca gtgtcaccac catggcgatc agcagccaga cgatcggacg ttcttcgcgc
     2281 cagttggaga tctgcatggc gaccatcgcg ccggcgatga tcgcgatcgc atcgccgatg
     2341 ccgtagccga ccaccgacca ggtttccatg atgtcgtcgc ccgacatggc ggcgaccggc
     2401 gccaacgccg tgaaccacag cagcgagccg aacccgattg caaccgtggt gaagtcgatc
     2461 cacgcgcgca tcgtgcgcgc acgtccgccc aggtcgaggt acaacagcac gaacgcccag
     2521 gccgcgaggg ggtaatagaa gatgtagagc acatcgggcc aggagccgaa ggtgacgttt
     2581 tcggtcagcg ccgtatatcc ccagccgaca ctcgcgatga gatcgagcac cagcgcggcg
     2641 aagatgagac gccacgcgcg acgtcgccgt tgactcagcg ccgcatcgaa cagggacggc
     2701 cacaacacga cacagatccc gacgctcacg gggaatgacc cccaggcgcc gatgtaatcg
     2761 gtcaccgctt cgccccccca tccggcgacc atgaacaaca tgcagacagc cgtgtagacc
     2821 actgagatcg cgaacagcgg ccgcgcccat cgcccgacaa atcttgcaat aaacgaattc
     2881 attcgccggt caatgctccc aaagcagcac ggcatccgcg ctgatcgcag tcacaacaac
     2941 atcggcatcg gggcaccgat ttgcagcttt ttcgccgtga cccggttgcc agtcccggaa
     3001 cgactcggca gactgcccgc ggcataaaga aagccctgag agaaaccctg ccggtgaatg
     3061 gacacaagcg aacgctgatt gtgattgcct tcacagcatg cggcgccgtc tgtgcgcatg
     3121 tcgcgcacgc cgacgccgtg caggtggttt ccggaagcat cggtgggacg tcggatttcg
     3181 tgttccgcgg actgtcactg acgcgcggca aacccgcggc gcaggcctcg atcgacgtgg
     3241 aattccccaa ggagttttac atcggcggct tcgtttccac ggcggatccg aagtccaatc
     3301 ccagccccga cctggaattg gatctgtggg ccggccgtta ctggcgcctc tcggaaaact
     3361 tttcgggcga cctgcgcctg tcgcagtaca cctatcccaa cgatccgcga cgagtcagtt
     3421 acaaccgcac cgaactcacc gccacgctgg gtcttcacaa ccggtttttt gccgcggcta
     3481 tctattcgcc caacacgtcg gcaatcggct cctcggctgg ctacgacgaa ggtaacgcgt
     3541 gggcactcga actttccggc cgccagccga tcaaccagca cctgtcgatc tctgcgggct
     3601 tcggtcacta cggtctcgaa gaggtctatc acgacagcta caactactgg aacgtcacgc
     3661 tgatcgcggc gttcaaggcc ttcgagttcc agttcgccta cctgggggtg gatgactccc
     3721 tggaacagca cttcgaaccg gactccgttg gcgatcgctt cgccgtcacc gcgttatggc
     3781 gtttctcgac caccaaatag cgttgcggga accgtatttc cggtccgcgg tactcatggg
     3841 cagtaggtcg atgaaggtgg gctgccgtgg cactgaaaag cgttgaattc cagtccccgc
     3901 gggcaccagg cgcaagtccc gacgcctctg aacgcgagct gctcgaacgc atagcgcggt
     3961 cgaaggacca ggcggcgttc cgcacgttgt acggcagcta ctaccagcgc ctttcgcgcc
     4021 tcctggcgcg aatgtcggtg cgccgcgaag acatcgaaga ggtcatcaac gacaccttct
     4081 gggtggtgtg gaccaaggcg ggcgagtttc gtggcgcttc gcagctgtcc acctggatca
     4141 tcggcatcgc ctaccggcgg gcactgaatg cgctgcgccg cgcgaagatc cggcccgtgt
     4201 ccgatgaacc gtttgatgaa gattcgatca gcgtgatgtc caccgacgcc gccgagaccg
     4261 atggccagtg gctggcgctg gggctcacac gcctgccggt cgagcagcgc atggcgctcg
     4321 aactgaccta cactctcggc cattcctgcg aagaggtggc cgcgatcctc gattgcccgg
     4381 tgaacaccgt gaagacacgc ctgttccggg cacgcgagac actcaaacaa gtgttgcccg
     4441 cccttgcggg catgtccgga gaaaaatcat gagcgcccgc attcccgata ctcacgccga
     4501 agcctgggct ctgctgccct ggctcgccaa cggccgcctc ccggcgtccg accgtgaatg
     4561 ggtggaggca catgtagctc attgcgccga atgccgcggt gagctcgacg cgcagcggaa
     4621 agtcgcaacc cagatcagcc gggacacttc gccggacatg gcggggaccc ccggctcgac
     4681 aggatacggc tcgacaggaa acgatgagca gcgctcgttc aacaagttgt gggcgcgcat
     4741 cgaggcgtcc gaaggtgcag cgccatcggc cagcggcgtc gccggcggct tctcctcccg
     4801 aacctcacgc accgtgcgat ggctcgcggc ggccgtggtc gtgcagggtt tcggcctggc
     4861 gctgttcggc ttcaacgcgc tgcgtgacaa cgccggcgaa atcgtcacgg tcgccgaagt
     4921 tcccgcacag cggctcaatg cgccggccgt ccgcgtcgta tttgcgccgg aagcatcgat
     4981 ggccaccatc aatacgctgc tcacgcacca ggagctttca atcgtgtccg ggccaggcac
     5041 gtcgggaaat ttcaccgcag aactgtcggc caaagccgtg gcctccggcg catcggcaga
     5101 ttccgtcgcc gcggtgttgt cgaaggatcc gaatgtcact ttcgcccaac cggttgcgcg
     5161 ctgatacgca catggactgg cggctcggcg ttgccgtaac gctggccgcc ctcgcgtttg
     5221 cgctgtcggg tggcgccatc gcggcggcgc cgccggttct cacgccgccg gaagttcgct
     5281 ccgaccccgc ccagttcatc gtcgtggccg tcgccaatcc ggtgacgggc cggcccggcg
     5341 cggtcggtgg caccgcccac ggttatggct cgagtacgaa ctaccgggtc agcgcgtcgg
     5401 caacggccat catccgcgag atcacctccc ggtattcact tacccgcgtt tccgagtggc
     5461 cgatcgacga actgcagatg cattgcgtgc tgttccggat tcctcccggc accacgcgcg
     5521 aagcgatcat cgaaaagctg aaggcggacc gccgcattct catcgtgcag ccgctcaacg
     5581 aattcgagtc cgccaccgaa acgccggtgg cagcacctgc cgtgacgttc gacgacccct
     5641 actcgcgcct gcaaaccaac gtacgcgctc tcgacgtggc ggaggcacac aacttcagcc
     5701 gcggcgccgg cattcgcgtg gccatcatcg acaccggcgt cgatacgcgt catcccgacc
     5761 tggtcggccg cacgcatctc acgcgcaact acatcgactc cgacgaagtc gcgtttcgcg
     5821 gcgaccgcca cgggacgcag gtggccgggc tcattgcggc cgcggccaac aacggggtcg
     5881 gcattgtcgg ggtcgcgcct gacgtgcggc tcatggccta caaggcctgc tggcaggcat
     5941 ccgccagcac gacggggcgc tgcaattcat tcaccattgc ccaggcgctc gcggatgcat
     6001 tggccgcaaa ggcgcaggtc atcaacctga gcctggtggg gcccagcgat ccgctgctcg
     6061 aggccctggt tgccaaggcc atcgcggcgg gagtgatcgt ggtgggcgcg gtttcggatg
     6121 atccccggtt cgcatttgcc gccaaactac cgagcgtgct gccggtcgcc gaatcggaaa
     6181 cctcggccaa ccagggcgac gagatcctgc gcgcgccggc acgagacatc gtcacgctgg
     6241 tcccgaacgg gcattacgac tttgcttccg gaagctcgct ggccactgcg cagatttccg
     6301 gagtggtagc gttgctgctg gcgcgcaatc agaaactcgg catcagccgc ctgcgcgagc
     6361 tgctgtctca atccaccgaa aaccacgaca cgactcgcgg acctttccgc tcggtcaatg
     6421 cctgcgtggc cgtcgccagg ctcgtgcgcg gcgccagctg caattgatgg gggcattcct
     6481 cttttgctgg caagagggga cgtggttcgt atgtggaggg tcgagcgacg ggcggccctg
     6541 catgcgatga gactcgcttt tcgccgggcc cttcgttgat tgactgccgc cagattgcgg
     6601 gccgccgaca cacacgaaga catcttgtag tcagttgagt tgggcggcag caaagagtcc
     6661 tgaacgcgca gtgatcggct tcgtg
//
LOCUS       DASQJS010000246         4328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2653439, whole genome shotgun sequence.
ACCESSION   DASQJS010000246 DASQJS010000000
VERSION     DASQJS010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2653439"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VEW08_09960"
     CDS             complement(<1..770)
                     /locus_tag="VEW08_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HYJ41106.1"
                     /translation="MKYTDYYAALGVGRGASADEIRKAYRRLAQKHHPDVSKEPGAEA
                     KFKEVAEAYQTLKDPEKRAAYDALGSRPQGEEFRPPPDWARQHGGAGAGSAQFSFDDA
                     DFADLFSRFGARSGALPGEDFEVPVEISIEDAFLGTTLSLSLSLPDYDAAGRMRRVPH
                     TVKARVAPGAVDGQRLRLPGKGGKGRHGGRDGDLYLDITLKPHRLYRTTGHDLYLDLP
                     LAPWEAALGATVEIPTLSGAVSLKVPAGTSAAKKLRLAG"
     gene            complement(784..1563)
                     /locus_tag="VEW08_09965"
     CDS             complement(784..1563)
                     /locus_tag="VEW08_09965"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HYJ41107.1"
                     /translation="MKRIFLVVLGALGAQAAGAGVYVELVDRDIASGSTELSQRMYVQ
                     NGLGRFVDAGGRATLIKNGTLYIIDDADKSYIAFDKATMEKLATKINAAMAQMKDQLA
                     KLPPEQRAQMEQAMPGMSGADQKWVVEALDTGKSDTVDGRNCRLWDVKRNGELDDQLC
                     VVPYSSLPGKENFQAVFANFARVFEEMAKSVPMLAGMMTNEFGAQAKVNGFPVRTRGY
                     ENGKLGNTEHKVSLWREEAIPASMFEVPADYKQKQMPMGGQ"
     gene            1624..2505
                     /locus_tag="VEW08_09970"
     CDS             1624..2505
                     /locus_tag="VEW08_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ41108.1"
                     /translation="MEERRKPVLGRVVPGVTPALQRRLRRVFAVLQWLSVPLASKLAF
                     RLFLTPPRRKVEPGDAPIIALAERATLRCGADKFTVWHWGQGDPCVVLLHGWGSHAAR
                     FATFVAPLRAAGFSVIGIDAPAHGTSPGRLSDLPRFRDSLAEVLRRHAPVHAVIGHSL
                     GGGAVLTVLAETADYHPKKICLFGVPSDMDFILGSFAMMLGLRYAARASLRERFAAHF
                     GRPAAAISTAAAAASVRIPVLIVHDEEDNVAPIAQGTALAAAIPHAMVWRTQGLGHSG
                     ALRDPATIERVVKFLCE"
     gene            complement(2536..3486)
                     /locus_tag="VEW08_09975"
     CDS             complement(2536..3486)
                     /locus_tag="VEW08_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41109.1"
                     /translation="MRLGLILLLCLLNTGCALWRRDREEPVANGPREEKAEPVLKPSE
                     ALKPKSLEVASPVSDRFFVRMSAFGASPETNLRVDPTSPAVFIIPPPPAPQVPINLPP
                     PGTELVAEEDLGLDDQIEQARMEFDIRMGERNHLRIDYFKIARFGEVIVTDPIDFGDS
                     TFDTDDIVRTNFDWRVLSLTYTYSLFKAERFEAGAGIGVHVIEAHAEMKQPGTLNNEK
                     FDEVAPFATIAFNASYRISKRWSITARGQTFEYSKDEITGRLSDFHGDIQFRWHKNLA
                     VGIGYTQLRYELEREPEPSNKPALFDMSVPGPEIFFRASF"
     gene            complement(3671..>4328)
                     /gene="rho"
                     /locus_tag="VEW08_09980"
     CDS             complement(3671..>4328)
                     /gene="rho"
                     /locus_tag="VEW08_09980"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017845130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HYJ41110.1"
                     /translation="EVHLIVLLIDERPEEVTEMQRTVKGEVVSSTFDEPAARHVQVAE
                     MVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVPSSGKVLTGGVDANALQRPKRFFG
                     AARNIEEGGSLTILATALIDTGSKMDDVIYEEFKGTGNCEIHLDRRIAEKRVFPAMNI
                     NRSGTRKEELITDPGELAKIWILRKLLHPMDELAAVEFLLDKMKDTKTNSEFFEAMKR
                     "
BASE COUNT          713 a         1418 c         1350 g          847 t
ORIGIN      
        1 cctgcgagcc gaagcttctt tgccgccgaa gtgcccgccg gcactttgag gctcacggcc
       61 cccgacaacg tcgggatctc caccgtcgcc cccagcgctg cttcccacgg cgcgagcggc
      121 agatccaggt acagatcgtg gcccgtggtg cgatacaggc gatggggctt gagcgtgatg
      181 tcgaggtaca agtcgccgtc gcggccacca tgcctgccct tgcctccctt gcccggcagg
      241 cgcaggcgct ggccgtcgac ggcgccgggc gcgacgcgcg ctttcaccgt atgcggcacg
      301 cgccgcatgc gtccggcagc gtcgtagtcc ggcagggaaa gcgacagtga caacgtggtg
      361 ccgaggaacg catcttcgat gctgatttcc accggcactt cgaagtcctc gcccggcagc
      421 gcgccgctcc gcgcgccaaa gcgcgagaac agatcggcaa aatccgcgtc atcgaacgag
      481 aactgcgcgg agcccgcgcc cgcgccgcca tgctggcgcg cccagtccgg cggaggccgg
      541 aactcctctc cctgcgggcg gctgccaagc gcgtcatagg ccgcacgctt ctcgggatct
      601 ttcagcgtct gatacgcctc ggcgacttcc ttgaacttcg cttccgcgcc cggctctttg
      661 gagacgtcgg gatggtgttt ctgcgcgagg cgccggtaag ccttcctgat ttcatcggcc
      721 gaagcgccgc gcccgacacc aagcgcggcg tagtagtccg tgtatttcat cgcgccccgt
      781 cgcctattgg cctcccatgg gcatctgctt ctgcttgtag tccgcgggaa cttcgaacat
      841 cgaggccggg atggcttctt cgcgccacag gctcactttg tgttcggtat tgccaagctt
      901 gccgttttca tagccgcgcg tgcgaaccgg gaaaccattc actttggcct gcgcgccgaa
      961 ttcattggtc atcatgccgg ccaacatcgg gacggatttc gccatttcct cgaacaccct
     1021 cgcgaaattg gcaaacacgg cctggaaatt ttccttgccc gggagcgacg agtacggcac
     1081 cacgcacagc tggtcgtcga gctcgccatt gcgcttgacg tcccagaggc ggcagttgcg
     1141 cccatctact gtgtcggact tgccggtgtc cagggcttcc acgacccact tctgatcggc
     1201 gccgctcatg ccgggcatcg cctgctccat ttgcgcgcgc tgctcgggtg gcagcttcgc
     1261 gagctgatct ttcatctgcg ccatggctgc gttgatcttc gtcgcgagct tctccatggt
     1321 ggccttgtcg aacgcgatgt agctcttgtc cgcatcgtcg atgatgtaca acgtgccgtt
     1381 cttgatcagc gtcgcccggc cgcccgcgtc gacaaatcga ccgaggccgt tctggacgta
     1441 cattctctgc gaaagctcgg tgctgccgga cgcgatgtca cggtccacca gctcgacgta
     1501 aacacccgcg cccgccgcct gcgcgccaag tgcccccagg acaaccaaaa aaatgcgttt
     1561 catgctttcc ctcgcggttt agtgctcggg actctacacc gcgcgatata gacttcaaac
     1621 cgcatggaag agcgccgcaa accagtcctg ggacgcgtgg ttccgggcgt aacgcccgcc
     1681 cttcagcggc ggctgcgccg cgtattcgcc gtgctgcagt ggctcagcgt gcctctggcg
     1741 agcaagctcg cctttcgcct ctttcttacg ccgccacgcc gcaaagtgga gcccggagac
     1801 gcacccatca tcgcgctggc agagcgcgcc acgctgcgtt gcggagcgga caaattcacc
     1861 gtctggcact ggggccaggg cgacccctgc gtggtgctgt tgcacggctg gggcagtcac
     1921 gcagcccgct ttgccacttt cgtcgcgccg ctgcgcgcgg cgggcttttc cgtgatcggc
     1981 atcgacgcgc ccgcgcatgg cacatcaccg gggcgcctgt cggacctgcc cagatttcgc
     2041 gacagcctcg cggaagtcct gcgccggcac gcgccggtgc acgcggtgat aggccattca
     2101 ctcggtggcg gcgcggtgct caccgtgctg gcggagacgg ccgactacca cccgaagaaa
     2161 atctgcctct tcggtgtgcc gtcggacatg gatttcatcc tcggatcgtt cgcgatgatg
     2221 ttgggactca ggtacgcggc gcgcgccagt ctgcgcgagc gcttcgccgc tcatttcggc
     2281 cgccccgcgg ccgccatttc aacagccgcc gccgcggcgt cggtgcgtat tccagtgctc
     2341 atcgttcacg acgaggaaga caatgtcgcg cccatcgccc aggggaccgc gctcgcggct
     2401 gccattcccc acgcgatggt gtggcgcacg caggggctcg gccacagcgg cgcgctacgc
     2461 gaccccgcca ccatcgaacg cgtggtgaag ttcctctgcg agtgacgcgc gggtagttag
     2521 cccgcggcga gccgttcaga agcttgcgcg gaagaagatt tccgggcccg gcaccgacat
     2581 atcgaagagc gcaggcttgt tgctcggctc cggctcccgc tccagctcgt aacgcagctg
     2641 ggtgtagccg atacccacgg ccaggttctt gtgccagcgg aactggatgt cgccgtggaa
     2701 atcggacagc cggccagtga tttcgtcttt cgagtactcg aatgtctggc cacgcgcggt
     2761 gatcgaccag cgcttcgaaa tgcggtagct cgcgttgaac gcgatggtgg cgaaaggagc
     2821 cacctcgtcg aacttctcgt tattgagggt gcccggttgc ttcatttcgg catgagcttc
     2881 gatgacgtgc acgccaatgc cggcgccggc ttcgaagcgc tcggccttga acagcgagta
     2941 cgtgtaagtc agcgacaaca cgcgccagtc gaagttggtc cggacaatat cgtcggtgtc
     3001 gaaagtcgag tccccgaaat caatcgggtc cgtcacgatc acttcgccga atctggcgat
     3061 cttgaaatag tcgatacgca gatggttgcg ctcgcccatc cggatgtcga attccatgcg
     3121 tgcctgttcg atctgatcgt ccaggccaag gtcttcctcg gccacgagct cagtgcccgg
     3181 gggcggcaaa tttatcggta cctgtggcgc gggtggcggc gggatgatga agaccgccgg
     3241 cgacgtcggg tcgacgcgca agttggtctc gggagacgct ccgaatgcgg acatgcgcac
     3301 gaagaagcga tcggagactg gactggcgac ctcgagtgac tttggcttga gcgcctcgct
     3361 gggcttgagc acgggctccg ccttttcctc gcgcggtccg ttcgcgaccg gttcctcacg
     3421 gtcacggcgc cagagcgcgc accccgtgtt cagaaggcac agaagaagga tcagcccaag
     3481 acgcattcgc ggctcctgcc caaacaagca aacggccctg gattcagggc cgcgccatga
     3541 tctaacagct cggggacggg cgccagcgtc gcggtcaagc gacgcgcccg gtgtgcgcta
     3601 cgtcacaccg taggcgggtg gggacatgga agagagtgtg gggacagact ggaagtctgt
     3661 ccccacccgt ctatctcttc attgcctcga agaactcgga gttggtcttc gtgtccttca
     3721 tcttgtcgag caggaactcg accgcggcaa gttcatccat gggatgcagc agcttgcgca
     3781 ggatccagat cttggcgagt tcgcccggat cggtgatgag ctcttccttg cgcgtgccgg
     3841 aacggttgat gttcatggcc gggaacacac gcttttcagc gatgcggcga tccaggtgga
     3901 tttcgcagtt acccgtgccc ttgaactctt cgtagatcac gtcgtccatc ttcgagccgg
     3961 tgtcgatgag ggccgtggcc aggatagtta ggctgccacc ctcttcgatg ttgcgcgccg
     4021 cgccgaagaa tcgcttcgga cgctgcaacg cattcgcgtc cacgccaccg gtgagcacct
     4081 tgccggagct cggcaccacg gtgttgtagg cgcgcgcgag gcgcgtgatg gaatcgagca
     4141 ggatcaccac gtctttcttg tgctcgacca ggcgcttggc cttctcgatc accatttccg
     4201 cgacctgcac gtggcgggca gcgggttcgt cgaacgtgga cgacaccact tcgcccttca
     4261 cggtgcgctg catctcggtc acttcttccg ggcgctcgtc gatgagcagc acgatgaggt
     4321 gcacttcg
//
LOCUS       DASQJS010000247         6647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3595519, whole genome shotgun sequence.
ACCESSION   DASQJS010000247 DASQJS010000000
VERSION     DASQJS010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3595519"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VEW08_09985"
     CDS             <1..559
                     /locus_tag="VEW08_09985"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HYJ41111.1"
                     /translation="LVLSWIVGLTAPLFTITGTEISGRDLILIAGGVFLVWKSTMEIH
                     QLTEGEEGHASKSVKASFGAIIVQIIIIDMVFSLDSIITAVGLVDELAVMIAAVVASV
                     GLMMLFARAIGDFVSAHPSIKMLALSFLLLVGVMLIAEGFDHKVPKGYIYFAMAFSVA
                     VEMLNIRMRKKKVAPVKLHEPYEKG"
     gene            562..768
                     /locus_tag="VEW08_09990"
     CDS             562..768
                     /locus_tag="VEW08_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41112.1"
                     /translation="MVFALLRLAVFIWIFGFIVLALVAVSSYLYSTEPEPERTRRVQT
                     RVRMALMWPVALMSSAGRARLRRG"
     gene            890..1651
                     /locus_tag="VEW08_09995"
     CDS             890..1651
                     /locus_tag="VEW08_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HYJ41113.1"
                     /translation="MIALFVLFSVGCGIKVVDTGQRGVKTRFGEITSTSLPEGLYFYN
                     PITSKIIEIDTRVKRLDGETESYTRDVQQANIKYTLNYRLQQNQAHTMYRDVGTDWEQ
                     RLIPQVVLGTLKEVVGKWDAVDLISNRDKATNTAFEQIRKNLAERFVEISRFEITDIS
                     YTTEFETSVEQKVIAQQKAIEEQNRTKQIEEQARQKVLSAEAEAKSMQIRAEALEQNA
                     KLVEWEAVQKWNGVLPQYMLGSGATPFINLNPGKN"
     gene            1659..2315
                     /locus_tag="VEW08_10000"
     CDS             1659..2315
                     /locus_tag="VEW08_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HYJ41114.1"
                     /translation="MTDASLPALIFDCDGVLIDSEYLASRVESELVRELGLMLDVEET
                     HDLFLGKTVDGVLDAIAARAGKRPSTAWVYNWAFATAHAFLRELKSVDGVGVAVETLR
                     RRGHLMAVASQSPLARVQLSLHVAGLAGQFGDHIYVTTMVARPKPAPDIYLLAAERLG
                     VAATNCIVIEDSPAGAAAALGAGMRVIGYAPGKTFDAMRASGARVIRAMDELISVIDA
                     "
     gene            2456..4000
                     /locus_tag="VEW08_10005"
     CDS             2456..4000
                     /locus_tag="VEW08_10005"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYJ41115.1"
                     /translation="MNRSLVCAALASFVLSLSACGGGGGSSSTPPPPPPPPPADTVAP
                     SVPAGVTAVAQSASSILVSWTASTDATGISGYRVFRDGGATAVSTVTTTSYTDAGLAA
                     STAYSYTVVAVDSSPAANASAASSAVNATTLAPPPSTPVRIAAQRVFPNLTLFAAHSL
                     LRVPHDTTRWAVVQQDGHVLSFADTPSVSTTSNLLDISDRVVFRNVHGLLGLAFHPNY
                     PTDTRAYAAYTHEVSAGVIVLRISEFTTADGGATLNAASEQMLFEMAQPGGHNNGGHL
                     LFGPDGFLYLGIGDGGNDDSATGVAGNGQLTNNLLGKILRVDVSGSTGSRRYRIPADN
                     PFAANPLCNLDGTGSQDCPEIFAWGFRNPWRWSFDSQGGQLWLGDVGSHAREEVDRVV
                     RGGNYGWRCFEGTMQTTLNCGTPTTPLLPPVAEYVHPVGQSVVGGFVYHGTAIPGLVG
                     RYVFADYNLGQIWNIATDTAPTRTVVTADGWESGLNPASFAQDNDGELYIVDVRTASI
                     YKLVPG"
     gene            complement(4104..5048)
                     /locus_tag="VEW08_10010"
     CDS             complement(4104..5048)
                     /locus_tag="VEW08_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ41116.1"
                     /translation="MDRRNFLALGSVAAVAGSGIVSAQGTVPSRTASAPASKLLMRRI
                     PSSGETIPAIGLGTSGPFEVGSDEAARAPLREVLQGFFAGGGTLIDTSPMYSTAEAVL
                     GDLLSADQQAKAFIATKVWTPGSGVHGAQKGVEQMQRSISLLRHKPIDLMQVHNLVDL
                     DAHLKTLGRWKAEGRVRYIGVTHYTTSSYPDLIAIIEREKLDFVQFNYSVTTREAEKR
                     LLPLCADKGVAVLVNRAFEDGNLFSHVQGRPLPAWAAEFGAKSWAQVFLKFVLAHPAV
                     TCVIPATGKLRNLVDNLGAATGALPDAKQRAQIIAEFK"
     gene            complement(5074..>6647)
                     /locus_tag="VEW08_10015"
     CDS             complement(5074..>6647)
                     /locus_tag="VEW08_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HYJ41117.1"
                     /translation="FCDSGWPALRAGSEFGGQGVPNVLGTAVEELWASANLAFKLCPM
                     LTQGAIEAIQHFGSPQQKQLYLPKMVSGEWTGTMNLTEPQAGSDLAQVRTRATPNGPN
                     YRLYGQKIFITYGEHDLTENIVHMVLARIDNAPPGVRGISLFIVPKYWVNADGVPGAR
                     NDVNCASIEHKLGIHASPTCVMMYGENEGALGLLVGEPNRGLEYMFVMMNAARLSVGL
                     EGYAQGERAFQQAVEWARTRVQGKPPVPLARISGSASMGTSSSPAPIIGHPDVKRMLL
                     GMKSTVEACRALALYAAHQLDIGAGHPDEAVRAAHELRGDLLIPIVKGFCTECGIEVA
                     SVGIQVHGGMGYIEETGAAQTFRDARITSIYEGTTGIQSNDLMGRKFGRDRGAAMLAF
                     IDDMEASLAKLAPLHASALHSRSAAQDALVRLRKTSETMLKALATAPDRAMAVSVPFL
                     KFCGLTIGGWLMARAADVAAQRLATGTSDREFFEAKLATAHFYASQVLPQVLTLEHIC
                     TQGSDAVSGTDVTLI"
BASE COUNT         1137 a         2158 c         2121 g         1231 t
ORIGIN      
        1 gctggtgctg tcgtggatcg tgggcctcac cgcgccgctg ttcaccatca ccgggacgga
       61 gatttccggc cgcgatctca tcctcatcgc cggcggcgta ttcctggtgt ggaagtccac
      121 catggaaatc caccagctca ccgaaggtga ggaaggccac gcctcgaaaa gcgtgaaggc
      181 cagtttcggc gccatcatcg tgcagatcat catcatcgac atggtgttct cgctcgactc
      241 catcatcacg gcggtcggcc tggtcgacga gctcgcggtc atgatcgccg cagtagtcgc
      301 ttcggtcggc ctcatgatgc tgtttgcgcg cgccatcggc gacttcgttt cggcgcaccc
      361 gtccatcaag atgctggcgc tgtcgttcct gctgctggtg ggtgtcatgc tcatcgccga
      421 agggttcgat cacaaggtgc cgaagggcta tatctacttc gcgatggcgt tctcggtggc
      481 ggtggagatg ctcaacatcc gcatgcgcaa aaagaaggtc gctcccgtca agcttcacga
      541 gccatacgag aagggttagt catggttttt gcactgctgc gtctcgcggt cttcatctgg
      601 atattcggct tcatcgtgct cgcgctggtc gccgtgtcga gttatctgta ctcgacggaa
      661 cccgagcccg agcgcacgcg gcgcgtgcag acgcgcgttc gcatggcgct gatgtggccg
      721 gtcgcactca tgtcatcggc cgggcgcgcg cgcctgcgcc gcggttaatc aggttcaggg
      781 agaatcgata atggctttca cgaataacgt ctttcgcggc ccgggcaacc cgggtaacgg
      841 cggagtccaa ctacccatgc gtagaatcct tatatgggcg gcagtcatct tgatcgccct
      901 tttcgtcctc ttctccgtgg gctgtggaat caaggtggtg gacaccggcc agcgcggcgt
      961 caaaacccgt ttcggcgaaa tcacctcgac gtcgcttccc gaaggtctct atttctacaa
     1021 cccgatcacc agcaagatca tcgaaatcga cacgcgtgta aaacgcctgg atggtgaaac
     1081 cgagtcgtac acgcgcgacg tgcagcaggc gaacatcaag tacacgctca actaccgcct
     1141 gcagcagaat caggcgcata ccatgtatcg cgacgtcggc acggactggg agcagcgcct
     1201 gattccgcag gttgtcctcg gtaccttgaa agaagtcgtc ggcaagtggg atgcggtgga
     1261 tctcatctcc aaccgcgaca aggccacgaa caccgctttc gaacagatcc ggaagaatct
     1321 cgccgagcgt ttcgtcgaaa tatcccgctt cgagatcacc gacatttcgt acaccacgga
     1381 gttcgagact tcggtggaac agaaggtgat cgcgcaacag aaggcgatcg aagaacagaa
     1441 ccgcaccaag cagatcgagg agcaggcgcg gcagaaagtc ctgtcggccg aggcggaggc
     1501 gaagtcgatg cagattcgcg ctgaggcgct cgaacagaat gccaagctgg tggagtggga
     1561 agccgtgcag aaatggaacg gcgtgttgcc gcagtacatg ctgggcagcg gcgccactcc
     1621 attcatcaat ctcaatcccg gcaagaactg agagtagctt gaccgacgcg agcttgccgg
     1681 cgctgatttt cgactgcgat ggggtgctca tcgacagcga atacctggcg agccgcgtcg
     1741 agtccgagct ggtgcgcgag ctcgggctga tgctcgacgt cgaggaaaca cacgatctgt
     1801 ttctcggcaa aaccgtcgat ggcgtgctcg acgctatcgc ggcccgcgcc gggaaacggc
     1861 cctcgaccgc ctgggtctac aactgggcgt tcgccacggc acacgccttc ctgcgcgagc
     1921 tgaagtcggt cgacggcgtg ggcgtggcgg tggagacgct gcgccgccgc ggccatctga
     1981 tggccgtggc ttcccagtcc ccgctcgcgc gcgtgcaact ctcgctgcac gttgccggcc
     2041 tcgccggtca gtttggcgac cacatctacg tcacgacgat ggtcgcgcgg cccaagccgg
     2101 cacctgacat ctatctcctg gcggcggaga ggctcggtgt cgcggccacg aattgcatcg
     2161 tcatcgaaga ctctccggcc ggtgcggccg ccgcgctcgg cgccggcatg cgggttatcg
     2221 gctacgcgcc cggcaagact ttcgatgcga tgcgggcgag cggcgcccgg gtcatccgtg
     2281 cgatggacga gctgatttcc gttatcgacg cctgagccgc gccgggcgcg cggcttactc
     2341 ctacagcaga ttcccctgac tcggccgcgc gagcgcctgc tatccgacgg cgccttggcg
     2401 ctcccggcgt cagattggac gctcgctgtc caaaaaacat aattcgggaa ttgccatgaa
     2461 tcggagtctg gtgtgcgcgg cgctcgcgtc gttcgtcctc tcattgtcgg cgtgcggggg
     2521 cggcggcggc agtagttcga ctccaccacc ccctccaccg ccaccaccgg ccgacacggt
     2581 ggcgccgagc gttcccgcgg gggtcaccgc cgtggcgcaa tcggcgagtt cgattctcgt
     2641 gagctggaca gcctcgaccg atgccaccgg aatttcgggt tatcgcgtgt tccgcgacgg
     2701 cggcgcgaca gccgtgagca ccgtgaccac gacttcgtat accgatgccg gcctcgctgc
     2761 gagcaccgcg tacagctaca ccgtggtcgc cgtggactcc tctccggcgg ccaatgcatc
     2821 ggccgcttcg tcggcggtga atgccacgac tctggcgcca ccgccgtcca cgccggttcg
     2881 aatcgccgcg caacgcgtct ttcccaacct cactttgttt gccgcgcatt cattgctgcg
     2941 cgtgccgcac gacaccacgc gctgggccgt cgtgcagcag gatgggcacg tattgtcctt
     3001 cgcggatacc ccgagcgttt ccacgaccag caacctgctc gacatcagcg accgggtggt
     3061 gttccgcaac gttcacggct tgttaggcct ggctttccac ccgaactatc ccaccgatac
     3121 gcgcgcctac gcggcataca cccacgaggt gagcgccgga gtcatcgtgc tgcggatctc
     3181 cgagttcacc acggccgacg gcggcgccac gctgaatgcg gcgtccgagc agatgttgtt
     3241 cgagatggcc cagcccggcg ggcacaacaa tggcggccac ctgttgttcg gtccggatgg
     3301 ctttctctat ctcggcatcg gtgatggtgg aaacgacgac agcgccaccg gcgtcgccgg
     3361 caatggccag ctcaccaaca acctgctcgg caagatcctg cgcgtcgatg tcagcggcag
     3421 caccggttcg cgccgctacc gcattccggc cgacaatcca ttcgcggcga atccgttgtg
     3481 caacctggat ggcactggat cccaggattg cccggagatc tttgcgtggg gttttcgcaa
     3541 tccgtggcgc tggtcgttcg acagccaggg tggccagctc tggctcggcg acgtgggctc
     3601 gcacgcccgc gaagaagtgg atcgcgtggt gcgcggcggc aactacggct ggcgatgctt
     3661 cgaaggaacg atgcagacca cgctcaattg cggaacgccc acgacgccgc tgctgccgcc
     3721 cgtggccgaa tacgtgcatc cggttggaca atcggtcgtc ggcggattcg tttaccacgg
     3781 caccgccatc cccgggctcg tgggccgcta cgtcttcgcg gactacaacc tggggcaaat
     3841 ctggaatatc gccaccgaca cggcacccac gcggaccgtc gtgaccgccg atggctggga
     3901 atccggcctg aaccccgcgt cgttcgccca ggacaacgac ggcgagctgt acatcgtcga
     3961 cgtgcgcacg gcctctatct acaagctcgt ccccggctaa cggatggtgc cgggccggat
     4021 tggagtccga caacgcgacg ccgtgggttc ggtggcgagt tatcccggtt tcccggcccg
     4081 gcaccatctg ttgacgccat ccgttacttg aactcggcga tgatctgcgc gcgctgcttc
     4141 gcgtcgggca gcgcgcccgt ggcggcgccc aggttgtcga ccaggttgcg gagttttccg
     4201 gtggcgggaa tcacgcaggt cacggccggg tgcgccagca cgaacttgag gaagacctgc
     4261 gcccagctct tcgcgccgaa ctccgcggcc caggccggta gcggcctgcc ctgcacgtgg
     4321 ctgaacaggt tgccgtcttc gaaggcgcga ttcaccagca ccgcgacacc cttgtccgcg
     4381 cacagcggca gcaggcgctt ctcggcctca cgcgtggtca ccgagtagtt gaactgcacg
     4441 aagtcgagct tctcgcgctc gatgatggcg atgaggtcgg gataagagct ggtggtgtag
     4501 tgcgtgacgc cgatgtacct gaccctgcct tccgccttcc agcgccccag cgtcttcagg
     4561 tgcgcgtcga gatcgacgag attgtggacc tgcatgaggt cgatgggctt gtgccgcaac
     4621 agcgagatgg accgctgcat ctgctcgaca cccttttgcg caccgtgcac acccgatccg
     4681 ggcgtccaca ccttggtggc gatgaaggct ttggcctgct ggtccgcgct cagcaggtcg
     4741 cctaacacgg cctccgccgt ggaatacatg ggcgaagtgt cgatgagcgt gccgccgcca
     4801 gcgaaaaaac cctgaagcac ctcgcgcagc ggcgcacgcg cggcttcatc ggatccgact
     4861 tcgaacgggc ctgacgtgcc gagcccgatc gctggaatcg tttcgcccga tgagggaatg
     4921 cgccgcatga gcagcttgct ggccggggcg gacgcggtgc gcgaaggcac cgtgccttgc
     4981 gcgctcacga tgcctgatcc cgccaccgcg gccacactgc ccagcgcgag aaaattcctt
     5041 ctgtccattt cgtcctcttg cgaaacgttt cgatcagatc agcgtcacgt cggttcccga
     5101 gaccgcatca cttccctgcg tgcagatgtg ctcgagcgtc agcacctgcg gcagcacctg
     5161 cgaggcgtaa aagtgcgcgg tggcgagttt cgcctcgaag aattcgcgat cgctcgtgcc
     5221 cgtggcaagc cgctgcgccg cgacatcggc ggcgcgcgcc atcagccacc cgccgatggt
     5281 caggccgcag aacttgagga agggcaccga cacggccatg gcccggtcag gcgcggtggc
     5341 cagcgccttg agcatcgttt ccgaagtctt gcgcagcctg acgagggcat cctgcgccgc
     5401 gctgcgggaa tgaagagccg aggcgtgcaa gggagcgagc ttcgcgagcg aagcctccat
     5461 gtcgtcgatg aacgccagca tcgcggcgcc gcggtcgcgc ccgaatttgc ggcccatgag
     5521 atcgttcgac tgtatgcccg tcgtgccttc gtagatcgag gtgatgcgcg catcgcgaaa
     5581 tgtctgcgcg gccccggttt cctcgatgta ccccatgccg ccgtgcacct ggatgccgac
     5641 cgatgcgacc tcgatgccac actcggtgca gaagcccttg acgatgggaa tcagcaggtc
     5701 gccgcgcagt tcgtgcgcgg cgcgtaccgc ttcgtccgga tggccggcgc cgatatccag
     5761 ctggtgcgcg gcgtacagcg ccagcgcccg gcaggcttcc accgtggatt tcatgcctaa
     5821 caacatgcgc ttgacgtcgg ggtggccgat gatcggcgcc ggcgacgaac tggtgcccat
     5881 cgacgccgaa cccgagatgc gcgcgagtgg caccgggggc ttgccctgca cgcgcgtgcg
     5941 tgcccattcc acggcctgct ggaacgcgcg ttcgccctgc gcataaccct cgaggcccac
     6001 ggaaagacgt gcggcgttca tcatcacgaa catgtattcg aggccgcggt tgggttcgcc
     6061 gaccagcagg ccgagcgctc cttcgttttc cccgtacatc atcacgcagg tcgggctggc
     6121 atggatgccg agcttgtgtt cgatggacgc gcagttcacg tcgttgcgcg cgccgggcac
     6181 gccatcggca ttcacccagt acttggggac gatgaacagc gagatgccgc gcacgccggg
     6241 cggtgcgttg tcgatgcgcg ccagcaccat gtgcacgatg ttctcggtga ggtcgtgctc
     6301 gccgtaggtg atgaagatct tctggccgta gagtctgtag ttagggccat tgggcgtggc
     6361 gcgcgtgcgc acctgcgcaa gatcgctgcc ggcctggggc tcggtcaggt tcatggtgcc
     6421 ggtccactcg ccgctcacca tcttcggcag gtacagctgt ttctgctgtg gcgagccgaa
     6481 atgctggatg gcttcgatgg cgccctgcgt gagcatgggg cagagcttga aggccaggtt
     6541 ggcggaggcc cacagctcct ccacggccgt gccgagcacg ttggggacgc cctgcccgcc
     6601 gaactcgctg ccggcccgca gcgctggcca gccgctgtcg cagaact
//
LOCUS       DASQJS010000248        19565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2970358, whole genome shotgun sequence.
ACCESSION   DASQJS010000248 DASQJS010000000
VERSION     DASQJS010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19565
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2970358"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VEW08_10020"
     CDS             complement(<1..617)
                     /locus_tag="VEW08_10020"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYJ41118.1"
                     /translation="MPRAIRIQETGGPEVMLLEEVEVPAPGAGQVQVEHTAIGLNFID
                     VYDRTGLYPQAMPGGLGREAAGLVSAVGKGVRGFRVGDRVAYVMSPPGTYADLRNVPA
                     ARVVKIPAGVSDEHAAVLALKGMTACFLLRHCFRVERGDVIVVHAAAGGVGSLLSQWG
                     RKLGAVVVGIVGSEDKVKLAKKNGCKHVLVRGRDEIAVSVRKFSKG"
     gene            complement(641..1951)
                     /locus_tag="VEW08_10025"
     CDS             complement(641..1951)
                     /locus_tag="VEW08_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HYJ41119.1"
                     /translation="MRALSIRVPAALGLLLCPLLSVAQAASATPSADTGDTAWIIAAT
                     ALVLFMTLPGLALFYAGLVRAKNVLSILLQCFAITGVVSLLWLAVGYSLTFSDGGAAQ
                     PFIGGLDKAFLFGVTREALSGTIPETLFFMFQMTFAIITPALVIGGFAERMRFSAVLG
                     FTVLWLLFVYVPLAHWVWGGGWLAKLGVMDFAGGIVVHVSAGVAALVCALVLGRRRGF
                     PETAMAPHSLPLTVAGAGMLWVGWFGFNAGSALAANGSAGMAMLTTHMGASAGALAWM
                     IIEWVKFGKPSMLGVVTGMVAGLGTITPASGYVGPLGAVFIGLSAGTICFFATQILKR
                     TLHIDDSLDVSPVHGVGGMVGTLLTGVFAAVSLGGVGYTVQQSMGAQVGVQLLGVGAA
                     AAWVAVVTWLILKLLNAMVGLRVSEEKETEGLDLAEHGERGYSP"
     gene            complement(2180..2518)
                     /locus_tag="VEW08_10030"
     CDS             complement(2180..2518)
                     /locus_tag="VEW08_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HYJ41120.1"
                     /translation="MKLVTAIIKPFKLDDVRAALSEIGVSGMTVTEVKGFGRQRGHTE
                     LYRGAEYVVDFVPKTKIEVAVRSDLTDQVVEAILKAAKTGKVGDGKIFITDIDRVIRI
                     RTGETDNSAL"
     gene            2753..4021
                     /locus_tag="VEW08_10035"
     CDS             2753..4021
                     /locus_tag="VEW08_10035"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ41121.1"
                     /translation="MAIHRIAIVGAGQAAAQAVETLHKRGHQGSITLVGDEALLPYQR
                     PPLSKKFLAGTLDRDRLLIRHAAHYTDHAVDLRLGFAVANIDRVRRRVEITDGSSVEY
                     DGLLLATGSHPRLLPLPGAELAGVHYLRTVADVDRLRAELAPGRRVVIIGGGYIGLEV
                     AATCREAGLNVTVLEAADRVMNRVVSPAVSAFYEAEHARHGVHILCGARVDALVPGRE
                     TGKTSEVFPVSRPGTYRVATVRLEDGHEVPTDFVLVAIGVLPTDALARAAGLECDNGI
                     LVDEYCRTSDPNIWAAGDCARHPSIHYRTRVRLESVDNAFEQGTSAALNMLGIRTLHD
                     KVPWFWSDQFDLKMVIVGLSTAHDEVVLRGDPASRAFSVCYLRDGELIAVETINHTKD
                     QMAARKLIPLRARPDLAKLADVSIPLKETV"
     gene            complement(4024..5214)
                     /gene="tal"
                     /locus_tag="VEW08_10040"
     CDS             complement(4024..5214)
                     /gene="tal"
                     /locus_tag="VEW08_10040"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HYJ41122.1"
                     /translation="MGNPLEALEFHGQSVWLDYIRRDLIADGELQRLIDEDGVRGVTS
                     NPAIFEKAIAGSTDYSDVLAEAGSRSLDAKAIYESIAVSDIRDAALVLLPTYDRTARR
                     DGYVSLEVSPKLARDARGTIEEARRLWKAVARPNLMIKVPATPEGIIAIRQLISEAIN
                     VNVTLLFSQDTYEEVVEAYLAGLEDRVASGADVEGIASVASFFISRIDSIVDERIKRQ
                     NLSRSLLGKVAIANARLTYRRYGELFEGPRWNALSYLGAQTQRVLWASTGTKNPEYRD
                     VLYVEELIGPDTVNTLPPATLAAFRDHGLVRASLFEDVEGAEETMSQLAKEGIPIKEV
                     TDLLLEQGLQLFSDAFDKLLAAVRQRSGMVVSGMVDLDTSTNPANSGLAPRISPSDKP
                     AAGR"
     gene            complement(5323..7389)
                     /locus_tag="VEW08_10045"
     CDS             complement(5323..7389)
                     /locus_tag="VEW08_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41123.1"
                     /translation="MSDASRATHTADYSPDFAAVEQAESAWIRERRASTGSVPQDAPL
                     LGLALSGGGIRSAAFNMGVLQVLARSGLLARTDYLSSVSGGGYIASCLTWLRAHVPAN
                     SSQHLGDVPLADGTGTVLDWLRAHGRYLITGKGLSGWTLAASILSGTLLNLFVLLPVI
                     LLLIGLASGDWFEFRWPPELRMPGAGAVPSHDGFMLIAIAGVALLALYLLATLAFALS
                     TILPPLRQLSAGQYLRQRLGQLLGGGLTFIGIGLLPVLAGIEETVAMFLRVDFAQELT
                     RHLTWIVPLITGMLSVRQANKQGEHAGMFAVAGLALVLCGLFTALYHLAHHTNLLATP
                     AFMAWLAASALFALVCDINAVSMHSYYRSRLAEAFLPEVGDDHGGAASHPMLFRLTDV
                     RAAAGGPFHIINTTLNTTSSTNAKLRARNGENMILTPLYCGSSATGYRRTSSYLRGEL
                     TLSTAFSVSGAAVDPDTYATKSRAVSFLMALLNVRLGIWVANPRHEPRRLRWPAWYHL
                     MMREMLGVGLDEKDARIHLADGGHFENLGLYELLRRRCRYIVVSDAGADPDTTLADLG
                     QAVQRARADLGASVEICADGLADGGTGLRTQAHLLGRVNYADGSHGEILYLKALLRKN
                     LGADIYGYWRTNPEFPNQPTSNQFYGEMQFDSYRELGRELMSGIVGDDANGDIERVFA
                     RWRKPA"
     gene            complement(7386..9695)
                     /locus_tag="VEW08_10050"
     CDS             complement(7386..9695)
                     /locus_tag="VEW08_10050"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HYJ41124.1"
                     /translation="MRRFLKWLGGLLVLGAVAGAGLYSWFVLWPVHSIPALEHVDEYV
                     WLDQGWGQGQNTPLREHYYYTAQGTSMPQGASVGAVRYSWFVNLELPLSRQRFAAPDH
                     MRRWRFLVDPAPTASNPDQLPIGFTRHFDPQIGEDVLDITCAACHTGEIHYTQSGKTR
                     AIRIDGGPAMHAFTDMSRGNFAPMLAASLLGTAVNPAKFDRFAEKVLGPGYPDAKPRL
                     RKALFDTLKAMGASGQNRRSKELYPVHEGFGRTDALGRIGNTAFGDHLSAANYQLGDS
                     PVSYPYVWNIWKFDWVQYNGSVSQPLARNVGEALGVGAIVPLVSSMGQPLPPGERFRT
                     SVDIRGLHRIEQTLQRLRPPDWPEQILGAVDRDKAARGKELFELRCQECHGPHLAETE
                     RSLANAPLKPNGVEWRMEVIPLDHIGTDPNAAMGFMNRRYDLSATGLSNAELQAALRP
                     LLTRQLLRDVRFRMREVVRLMIKEENKDNSAMVQRIPKEIVELYNLTRDHPDPDAAAN
                     PAIPAELFARIDAALVAPLPLPAKLPEVDWRPADPLDCALECHVVNLLWDLREGGKNI
                     ERTLAGLDVKSLTEGVALNLVGILIKNRYYADNGIDYATQQCIEGFGTLDLPQQIAGY
                     KPRPLEGVWATAPFLHNGSVPTLYQMLLPPAKRDTKFFVGRREYDPLHVGFVTTPDDD
                     GDDDGFWLDTSISGNHNTGHAFAADAPTWAKHLQDPKANPLPHGVIGPELTDEQRFDI
                     IEYLKVHRDLPATPADYQPPQCRLRGEIL"
     gene            9760..11535
                     /locus_tag="VEW08_10055"
     CDS             9760..11535
                     /locus_tag="VEW08_10055"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYJ41125.1"
                     /translation="MNRPGSVASTRTSWTSLLILLVSCLPIPVLGDSPAFISLDPGAP
                     VHVEELGDALELLEDRVGNLDVAAVSTAPVDARFVSATLESTNIGFSPSAWWARVTVR
                     NPSDASRLIYLRQDYPLIDSLDLYEPSGDGGWRVHSTGDRKPFGSRDVAHKDFLFPVT
                     LPANSDRTFYLRYASQGPVDINLSLLDPNEVAGELSREQVAYGLYFGCVLMLLVWSGL
                     VFLAVRDGAFLAYFAYVATFGLYMTVNTGFAFQYLWPDSPRWANTALIVLLNIALITA
                     LQFSLTILRARDYTPRLFVVARVLQAMALVAITLIPVFSYAVLVRPVTFLILVSVCFM
                     IALGVISLLLGSRPARFYVIAWGAFLCGSVVFLLKNFGLVPHTFVTQHSWQVGALLEM
                     ILLSMTLSSRMSELQHQSRTDPLTLLGNRRQFDINLPAEFARSMEQGQPLSLLVVDID
                     QFKLYNDLHGHAQGDEAVKAVAAALRKHARKPYIACRYGGDEFCAILPGTSEASAAVL
                     AERLRAAVQHSLTGEETVTVSIGYACQTGGRFENADQLFEAADAALYSAKEQGRNTSV
                     AFQGRRSNDPPSVKEDDALDVPRVG"
     gene            11618..12034
                     /locus_tag="VEW08_10060"
     CDS             11618..12034
                     /locus_tag="VEW08_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HYJ41126.1"
                     /translation="MTEPKLSALELRIMGELWSRGACSIREIHEGVVKRDRPAYTTVQ
                     TMVYRLEVKGAVRRTKKIGNAHVFEATTSRASVQTRLLKELLGLFGGNARPVMAHLVE
                     MGKISSEDIDEAQKLLRAHTAKEPAVKDALPKDRGR"
     gene            12031..13368
                     /locus_tag="VEW08_10065"
     CDS             12031..13368
                     /locus_tag="VEW08_10065"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HYJ41127.1"
                     /translation="MIAALVNHLWQSTLFCGGAWLITLALRANGAALRHWVWLLASLK
                     FLVPFSLLFYVGSYIGLPAARTADIPPLMLGDALQSVSLLVSPSALGATATGAGSIAL
                     VILLAVWLGGALLVGTRWLLAWRAADSLVRAARPAPGALPDARITDADIEPAVARVFH
                     PVVLLPAALLGRLSQREIDAVLAHEREHIARHDNLKDSVHRLVETLFWFHPAVWWIGR
                     QMIEERERACDEAVLEVGHDGGDYASGILAVCRHCSAGVAGTATATSGNLTQRVRYIL
                     GDGRPAALGAVKAISLMVATIAATTVPMFAGAVDGTAHRLELLAMNSRALGTAEFFIS
                     PTVNNLDSRHQVVAGRDGVVIRNTSLRDLIALSYGVERWQVRGNGQWLDSLRYDIRVV
                     TRDPVSEPDELDPYALRDLTAKLLATRFDLQIHVNRQCQSPCGRYARAANPTQ"
     gene            13373..14320
                     /locus_tag="VEW08_10070"
     CDS             13373..14320
                     /locus_tag="VEW08_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41128.1"
                     /translation="MITGLVDHLWQSCCCCGIAWIFSTLTRDNTAVVHLWLWRIAALK
                     LLLPFSLLFALGAWLGFPSAHSADPAPPDFVRAISLLAPLASPARVLGWTGLPLALAA
                     LAALAAAGACAYWLHDRLRIERERVIEEAARRERDIDDIQSRPGFICSALMSAVVLCA
                     VSIPMLAGAIHDRQQRLERLISNSLALRDAPVVMAPAARGLGTRYRVDADAHGVSVRN
                     ANIHDLVAIVYGVRRHSVITPQMYSGVESASRSWLHWPRYDIRIQAQVPDPSDFDPYA
                     LRQNLTRALSEQFGLEIYVNGDCQPPCGNYHLALANEPL"
     gene            14784..16997
                     /locus_tag="VEW08_10075"
     CDS             14784..16997
                     /locus_tag="VEW08_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ41129.1"
                     /translation="MPNTRRILLVQENAADARAVRDALTNSGPYALSVEWVKRCSQAV
                     ELLRGDETRGAEGFAAVLVDLFLSDSSGLDTFDRLFKAAPQLPILVLSNSQNEPIARQ
                     AVQRGAQDYLLKGRLDNYLLPKALGSMIDRAAHAEALFEEKERAQVTLNSIGDAVLSC
                     DVASRVTYLNKVAEQLTGWTGEKAAGRPVEDVFHILNASTRATIPNPMALAISENRTV
                     LLAPNSILLRHDGVEAAIEDSAAPIHDRHGRVTGAVMVFHDVSSTRALSLNLAHQAQH
                     DSLTDLPNRMLLDDRLGHSMVVARRHGVKLAVLYLDLDRFKKVNDSVGHDIGDKLLQI
                     VARRLLGCVRGSDTVSRLGGDEFVVLLSEVVAGQDAAISAEKILRALSGPFDIDGHHL
                     HVSASIGIAIYPEDGDDAVSLLKHADFAMYHAKDCGRNNFQFFREDMNVRALERQSIE
                     DGLRLALERDEFVLYYQPIMDLWSGSPTGVEALLRWRHPHRGLLFPQQFVPIAEECGL
                     ILPIGRWVLREACRQLRTWQDEGLAPLRIAVNVSAVELRHKDFVEGVRQCLSDCGLAA
                     QHLELELTETFLMQDSATTALVLKALKDLGVQLALDDFGTGYSSLSHLKGFPIDTLKI
                     DQAFVSELDSNPNDASIVRAVIGLGRNLNIRVVAEGVETAAQLARLQEQQCPEGQGYF
                     FSRPVIADELPRLLLTAPPGFPDLHDGVGDAFNGGPVVPGPTDAPPRHVSRSSRN"
     gene            complement(17013..18065)
                     /locus_tag="VEW08_10080"
     CDS             complement(17013..18065)
                     /locus_tag="VEW08_10080"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF007186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HYJ41130.1"
                     /translation="MSVSQIELITRPGLLTPSRSYKPFRYPWAIEYWRRQQQIHWMPE
                     EIPLGEDCKDWNNRITDAERNLLTQVFRFFTQADVEVQDNYMERYARVFRPTEIKMML
                     AAFANMETVHIAAYALLLETLGMPDSEFTAFMDYAAMRDKHDYLQGFGVDTEADTLRT
                     LAVFGGFTEGLQLFASFAILLNFPRHNKMRGMGQVVSWSVRDETLHCEGIIRLFHTFA
                     QETGALTQAVKDDIVDCARTVVKLEDRFVDLAFEMGPIEGLSAAEIKAYIRYIADWRL
                     KQLGLPQVYGVKEHPLPWLTAMLNGVEHANFFETRATEYAKAATGGAWHGGEGAWGLF
                     DRRVARRGQLDLLANS"
     gene            complement(18056..>19565)
                     /locus_tag="VEW08_10085"
     CDS             complement(18056..>19565)
                     /locus_tag="VEW08_10085"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF006577.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HYJ41131.1"
                     /translation="ENVWLASNGGGIGTYWGGVRSIGETVKGCGQTSGIIPFVRVMDS
                     LTLAISQGSLRRGSAAVYLDVFHPEIEEFIEIRKPSGDFNRKSLNLHHGICITDEFMQ
                     AVRDDLPFALRSPKDGKELRTLSARHLWQKILETRLQTGEPYLLFSDTANNQLAPHQR
                     ELGLKVRQSNLCSEILLPTGVDHLGRARTAVCCLSSVNFEAWEQWCTEPRFIEDVLRM
                     LDNVLQEFIDHAPASMDTAKYSAMRERSVGLGAMGFHSWLQNNGIPFESALARAANLR
                     MFKHLRREADAASRVLAEERGACPDAAERGTMERFSHKLAIAPTASISVICGGTSACI
                     EPIPANIYTHKTLSGSFSVRNPALQKVLGARGLNTEDTWQSILAHEGSVQHLEGLTDD
                     EKSVFRTAFEIDQRWVIELAADRAPLICQGQSLNLYLRSDIHKWDLLMLHWTAWERGV
                     KSLYYCRSKSVQRAGFAGVEADNTRSWPPKPLKGPLTSDTDEAKYDECLSCQ"
BASE COUNT         3523 a         6281 c         6161 g         3600 t
ORIGIN      
        1 cccttgctga attttcggac gctcaccgcg atttcatcgc ggccgcgcac cagcacgtgc
       61 ttgcagccgt tcttctttgc gagcttcacc ttgtcttcgc tgccgacgat gcccacgacc
      121 acggcgccta acttcctgcc ccattgcgag agcagcgagc cgaccccgcc agccgccgcg
      181 tgcacgacga tcacgtcgcc gcgttcgacg cgaaagcagt ggcgcagcag gaaacaggcc
      241 gtcatgccct tgagcgccag caccgcggca tgttcgtcgc tcacaccggc cggaattttc
      301 acgacacgcg ccgcgggtac gttgcgaaga tcggcgtagg tcccgggcgg cgacattacg
      361 tatgcaacac gatcaccgac gcgaaatccg cgcacgccct tcccaaccgc cgacacgagt
      421 cctgcggctt cgcgccccag gcctcccggc atcgcttgcg gatacagacc tgtacgatcg
      481 tagacgtcga tgaagttgag cccgatggcg gtgtgctcca cctgcacctg gccagcgccg
      541 ggagcgggga cttccacttc ctcgagaagc atgacctcgg ggccgccggt ttcctgaatg
      601 cgaatggcgc gtggcatcgg ctactgctcc tcgagtggga tcagggagag taaccccgct
      661 ccccgtgctc ggcgaggtcg aggccttcgg tttccttctc ctcggacaca cgcaatccga
      721 ccatggcatt gagcagcttg aggatcagcc aggtaacgac agcgacccag gccgcggccg
      781 cgccgactcc gagcaactga acgcccactt gtgcacccat gctctgctgc acggtgtagc
      841 cgacgccgcc gagcgatacg gcggcgaaca cacccgtgag cagcgtgccg accatgccgc
      901 cgacaccgtg caccggcgag acgtcgagcg agtcgtcgat gtggagagtc cgcttgagaa
      961 tttgcgtcgc gaagaagcag attgttcctg cggaaagtcc gatgaacacc gcgccgagcg
     1021 ggcccacgta gccggatgcg ggagtgatag tgccgaggcc ggcaaccatg ccggtgacaa
     1081 caccaagcat tgacggcttt ccgaatttga cccactcgat gatcatccac gcgagcgctc
     1141 cggccgaagc acccatgtgc gtggtaagca tggccatgcc cgcggaacca ttcgcggcga
     1201 gcgcgctgcc agcgttgaag ccgaaccagc ccacccagag cattccagcg ccagcgaccg
     1261 tgagcggcag gctgtgcggc gccatagctg tttccggaaa tccgcggcga cggccgagca
     1321 ccagcgcgca aaccagcgcg gcgacacccg cgctcacgtg cacaacaatg ccgcccgcga
     1381 aatccatgac gccgagtttg gccagccagc caccgcccca cacccagtgc gccaagggga
     1441 cgtagacaaa aagcagccag agaacggtaa atcccagaac ggcagagaaa cgcatgcgtt
     1501 cggcgaagcc gccgatgacc agggcaggcg tgatgatggc gaaggtcatc tggaacatga
     1561 agaacagggt ttcggggatg gtgccgctga gcgcttcacg cgtcactccg aacaggaaag
     1621 ccttatcgag tccaccgatg aaaggctgcg cggcaccgcc gtcggagaaa gtcagggagt
     1681 agccgacggc cagccacagc agcgacacaa caccggtaat ggcgaagcac tgcaagagaa
     1741 tggagagcac gttcttggcg cgaaccaggc cggcgtagaa caacgcgaga cccgggaggg
     1801 tcatgaacag gaccagggcc gtcgcggcaa tgatccaggc ggtgtcgccg gtatcagcgg
     1861 aaggcgtggc ggatgccgcc tgcgcaactg aaagtaatgg gcacagcagc aagccgagcg
     1921 ccgccggcac ccggatagat agagcacgca tttttcgatc ccctcttgcc agtggcttgc
     1981 gccacattct caaacgccgg cccgctgccc gaactagcag ggggtattgt tagtaatcag
     2041 cacgaacttc gaatcaacgg cctgccgctg gaagggcagc agtatcgtca ggggctcgcg
     2101 ccccactcac aaacgccgcc ccgctgctcg gaaggagcag cgggtcgtcg cagatgcaat
     2161 ttagaaagtc gttccgccct tacagggcgc tgttatccgt ctcgcccgta cggatacgga
     2221 tgacgcgatc gatatcggta atgaaaatct tgccgtcgcc gaccttgccg gtcttggcgg
     2281 ccttgagaat ggcttccacc acttggtcgg tgagatcgct gcgcacggca acttcaattt
     2341 tggttttcgg gacgaagtcg acgacgtatt ccgcgccgcg atagagctca gtgtggccgc
     2401 gctggcgacc aaagccctta acctccgtca ccgtcatgcc ggaaacgcct atctcggaca
     2461 gcgctgcacg tacgtcgtcg agtttgaaag gcttgatgat ggcggtgacc aatttcattt
     2521 cttttctccc aattctttag tgccagttct tcaaataaat tcggggacgc cgacgtcggc
     2581 gagccttaat cgcagtgcac cttccgtgcc agtatcccga aagcccacaa acgcggcaat
     2641 ttcgttattg gccgtccagg cagctttttc cagagcacta aattagcgcg cccaaacaac
     2701 tgccgcccaa gaatggtgcg gaaacccgga tgaagtactc tctcttatct atatggcgat
     2761 ccatcgaata gcgatagtcg gcgctggaca ggccgcagcg caagcggtcg agactctgca
     2821 caagcgcggt caccagggct ctatcactct ggtgggcgac gaagcgctgc tgccctatca
     2881 gcgtccgccg ctatcaaaga agtttctggc cggaacgctg gatcgggatc ggctgctcat
     2941 acggcatgcg gcccactaca ccgatcacgc cgtcgatctg cggctgggtt ttgcggtggc
     3001 gaacatcgac cgggtgcggc ggcgagtcga gatcaccgac ggcagttcag tcgaatatga
     3061 cgggctgctg ctggccaccg gcagtcatcc gcgtctgttg cctctgcccg gcgccgaact
     3121 tgccggtgtg cactacctgc gaaccgtagc ggacgtagac cggctgcgcg ccgagctggc
     3181 gcccgggcgg cgcgtggtca tcatcggcgg cggttatatc ggcctcgaag tggccgcgac
     3241 ctgtcgggaa gccggactca acgtaacggt gctcgaggcg gcggaccgcg tgatgaatcg
     3301 tgtggtcagt cccgcggtgt ctgcattcta tgaggctgag cacgcgaggc acggtgtcca
     3361 cattctgtgt ggcgcccgcg tggacgcatt ggtgccgggc cgggaaaccg ggaaaacttc
     3421 agaagttttc ccggtttccc ggcccggcac ctatcgagtt gccacggtac ggctcgagga
     3481 cggtcacgaa gtcccgacgg atttcgtgct ggtcgccatc ggcgtgttgc ccaccgatgc
     3541 attggcccgc gcggccggac tcgagtgcga caacggcata ctggtcgacg aatactgccg
     3601 gacatcggat ccgaacatct gggccgccgg cgactgcgcg cggcatccga gcattcacta
     3661 cagaacgcgc gtgcggctcg agtccgtgga caacgctttc gaacagggca ccagcgccgc
     3721 gctcaacatg ctcggcatcc ggacccttca cgacaaggtg ccctggttct ggtcggatca
     3781 attcgatctg aagatggtta tcgtggggtt gtccacggcg catgacgagg tggtgctgcg
     3841 gggagatccg gcgagccgcg ccttcagcgt gtgttatctg cgggacggtg agctgatcgc
     3901 ggtggagacc atcaaccaca ccaaggatca gatggcggct cgcaagttga tcccgttgcg
     3961 ggcacggccg gacctggcga agctcgccga cgtgagcatt ccgctcaagg aaaccgtctg
     4021 agcctatctg cctgccgcag gcttgtcgga gggtgagatc cgcggcgcca atccggaatt
     4081 cgcagggttg gtcgacgtgt ccagatcgac catcccactc accaccatcc ccgaccgctg
     4141 cctgaccgcc gcgagcagtt tgtcgaatgc atcactgaag agctgcaagc cctgctcgag
     4201 cagcaggtcg gtgacttcct tgatcgggat gccctccttt gccagctgcg acatcgtctc
     4261 ctccgcacct tccacgtcct cgaagaggct ggcgcgcacg aggccatgat cgcggaatgc
     4321 ggccaacgtg gcgggtggca gagtgttcac cgtgtcgggg ccgatgagct cttcgacgta
     4381 aagcacatca cgatattccg gattctttgt gcccgtactg gcccacaaga cccgctgggt
     4441 ctgagcgcca agatacgaga gagcattcca gcgtggtccc tcaaacagct ctccataccg
     4501 ccggtacgtg agcctggcgt tggcgatcgc caccttgccg agcaggctgc gcgagaggtt
     4561 ctgccgcttt atccgttcat ccacgatcga atcgatcctg ctgatgaaga aactcgcgac
     4621 gctggcaatg ccttcgacat ctgctccact ggcgacgcga tcctccaggc ccgccaggta
     4681 agcttcgaca acctcttcgt atgtatcctg cgaaaacagc agcgtgacat tgacgttgat
     4741 cgcttcgctt atcagttgac gaatggcaat gatgccttcg ggagtcgcag gcaccttgat
     4801 catgagattc ggccgcgcca ccgccttcca cagccggcgt gcttcctcga tggttcctcg
     4861 cgcatcccgc gccagctttg gcgagacctc caggctcaca tagccatcgc gccttgcggt
     4921 ccggtcgtat gtgggcaaca gcaccagggc ggcatcccgt atgtcgctca ccgcgatcga
     4981 ttcgtagatt gccttggcgt cgagcgagcg agagcccgcc tccgccagca catcgctgta
     5041 gtccgtgctg ccggcaatgg ccttctcgaa aatggcgggg ttcgacgtca cgccacggac
     5101 gccatcctcg tcgatgagtc gctgcagctc accatcggcg atcaggtcgc ggcggatgta
     5161 gtccaaccac acggactggc cgtgaaattc aagagcttcc agtggatttc ccatggcgta
     5221 ccagttgacc agtctccggc cggacgcgat ataaggatta gctccaattg cggtttgcgc
     5281 caggcaccgc gcaacattcg tttcccatct gccgtgaagc agttaggccg gctttcgcca
     5341 gcgcgcgaac acccgttcga tatcgccgtt cgcatcgtct cccacgatgc cactcatgag
     5401 ctcgcgcccg agctcacggt aactgtcgaa ctgcatctcg ccgtagaact ggttggacgt
     5461 gggctggttg ggaaactccg ggttcgtgcg ccagtagccg tagatgtcgg cgcccaggtt
     5521 cttgcgcaac aacgccttga gatacaggat ttcgccgtgg ctgccgtcgg cgtaattcac
     5581 gcgccctaac aggtgcgcct gcgtgcgcag tcccgtgccg ccgtcggcca gcccgtccgc
     5641 gcagatttcg acactggcgc cgagatccgc gcgcgcgcgc tgcaccgcct ggcccaggtc
     5701 ggcgagcgtg gtgtccggat ccgcgcccgc gtcgctcacg acgatatagc ggcagcgccg
     5761 gcgcagcagc tcgtacagac cgagattctc gaagtgcccg ccgtcggcga ggtggatacg
     5821 cgcgtctttc tcgtcgagcc cgacgccgag catttcgcgc atcatgaggt gataccaggc
     5881 cggccagcga agccgtcgcg gttcatgccg tggattggcc acccagattc cgaggcggac
     5941 gttcagcagc gccatgagaa aactgaccgc gcgcgatttc gttgcatagg tgtcgggatc
     6001 caccgcggcg ccggacaccg agaatgcggt cgacagcgtg agctcgccgc gcaggtaact
     6061 cgaggtgcgc cggtatccgg tggcgctcga cccgcagtac agcggcgtca ggatcatgtt
     6121 ctcgccattg cgtgcgcgca gcttggcgtt ggtcgaactc gtggtattca gcgtcgtgtt
     6181 gatgatgtga aagggcccgc ccgcggcggc gcgcacgtcg gtgagccgga acagcatcgg
     6241 gtgactggcg gcgccaccat ggtcgtcgcc cacttccggc agaaacgctt cggccagccg
     6301 gctgcggtaa tagctgtgca tggagacggc attgatgtcg caaaccagcg cgaacagcgc
     6361 cgaggccgcg agccacgcca tgaatgcggg cgtcgccagc agattcgtgt ggtgcgcaag
     6421 atggtagaga gcggtgaaca ggccgcagag cacgagcgcc agacccgcga ccgcgaacat
     6481 ccccgcgtgc tcgccctgct tgttggcctg gcgtaccgac aacatgccgg tgatcagcgg
     6541 cacgatccag gtgagatggc gggtgagctc ctgcgcgaag tcgacgcgca gaaacatggc
     6601 gacagtctcc tcgatacccg ccagcaccgg caacaatcca atgccgataa acgtgaggcc
     6661 cccgcccagc agctgaccga gccgctggcg caggtattga ccggcgctga gctggcggag
     6721 cggcggcaat atggtgctca gcgcgaacgc cagcgtggcc aatagataga gcgccagcag
     6781 ggccacaccg gcgatggcaa tcagcatgaa gccatcgtgc gagggcaccg cccccgcgcc
     6841 gggcatgcgg agctcgggcg gccagcggaa ctcgaaccag tcgccggagg cgagcccgat
     6901 cagcagcagg atgacgggca gcagcacgaa gagattgagc aacgtgcccg acaggatgct
     6961 ggccgcgagc gtccagcccg acagcccctt gccggtgatc aggtagcggc cgtgggcacg
     7021 caaccagtcc agcaccgtac cggttccgtc ggccagcggc acatcaccca ggtgctgcga
     7081 ggaattcgcc ggtacgtgcg cgcgcaacca ggtcaggcag gacgcgatgt agcccccacc
     7141 ggagaccgac gatagatagt cggtgcgcgc gagcagcccg ctgcgcgcca acacctgtaa
     7201 tacgcccatg ttgaacgcgg cggagcggat gccaccgccc gacagcgcca gcccgagcag
     7261 tggcgcgtcc tgcggcaccg acccggtgga ggcgcgacgc tcgcggatcc aggccgactc
     7321 cgcctgttcg acggcggcga aatcggggct gtagtcagcg gtgtgcgtcg cgcgcgacgc
     7381 atcactcaca agatttctcc acgcaaccgg cattggggtg gttgatagtc ggcgggagtc
     7441 gccggcagat cccgatggac cttgagatac tcgatgatgt cgaaccgctg ctcgtcggtg
     7501 agctcgggtc cgatgacgcc gtgcggcaat gggttggcct tcgggtcctg cagatgcttc
     7561 gcccaggtag gcgcgtccgc cgcgaatgcg tgaccggtgt tgtgattgcc ggaaatgctg
     7621 gtgtcgagcc agaagccgtc atcatcgcca tcgtcatccg gcgtcgtcac gaaaccgacg
     7681 tgcagcggat cgtactcacg ccggccaacg aagaacttcg tatcgcgctt cgcgggcggc
     7741 aacagcatct ggtacagcgt gggcaccgag ccgttgtgca gaaatggcgc tgtggcccac
     7801 acgccttcga gcgggcgcgg cttgtagccg gcgatctgtt gcggcaggtc gagcgttccg
     7861 aagccctcga tgcattgctg cgtggcgtag tcgatgccgt tgtcggcgta gtaacggttc
     7921 ttgatcagta tgccgacgag gttgagcgca acgccttcgg tgaggctctt cacatcgagc
     7981 cccgccagcg tgcgctcgat gttcttgccg ccctcgcgca gatcccacag caggttcacg
     8041 acatggcatt cgagcgcgca gtccagcgga tccgccgggc gccagtccac ctcgggcaac
     8101 ttcgccggca ggggcagcgg cgcgaccaag gccgcgtcga tgcgcgcgaa cagctccgct
     8161 ggaatcgccg gattcgccgc ggcatccgga tctggatggt ccctggtcag gttgtagagc
     8221 tccacaatct ccttggggat tctctggacc atggcgctgt tgtccttgtt ttcctccttg
     8281 atcatcaggc gcacaacctc gcgcatgcgg aaacgtacgt cgcgcagcag ctggcgcgtg
     8341 agcagcggcc gcagcgcggc ctgcagctcg gcgttcgaga gtccggtggc ggaaaggtcg
     8401 tagcgccggt tcatgaatcc catggccgca ttcgggtcgg tgccgatgtg atcgagcggg
     8461 atcacctcca tgcgccactc gactccgttc ggcttgagtg gcgcattggc cagcgagcgc
     8521 tcggtctcgg cgaggtgcgg gccatgacat tcctggcagc gcagttcgaa gagctccttg
     8581 ccgcgcgcgg ccttgtcacg atcgacggcg cccagtatct gctcgggcca gtctggcggg
     8641 cgcagacgtt gcagcgtctg ctcgatgcga tgcaggccgc ggatgtccac cgaagtgcgg
     8701 aaacgttcgc cgggcggcaa cggctgcccc atcgagctga ccagcggcac gatggcgcca
     8761 acgcccagcg cctcgccgac attgcgcgcc agcggctgcg acaccgaacc gttgtactga
     8821 acccagtcga acttccagat gttccacacg tatggataac tcaccggcga gtcgccgagc
     8881 tggtagttag ctgcggacag gtggtcgccg aacgcggtat tgccgatgcg gcccagcgca
     8941 tcggtacggc cgaaaccctc gtgcaccggg tagagctctt tggagcgacg gttctggccg
     9001 ctggcgccca ttgccttcaa ggtgtcgaac agcgccttcc tgagtctggg tttggcatcg
     9061 gggtaaccag gacccaagac cttttcggcg aagcggtcga acttcgccgg attgaccgcg
     9121 gtgcccagca gcgacgccgc cagcatgggt gcgaagttgc cacgcgacat gtcggtgaag
     9181 gcgtgcatgg ccgggccgcc gtcgatgcgg atggcgcggg tcttgccgga ttgggtgtag
     9241 tgaatctcgc ccgtgtggca ggccgcgcag gtgatgtcga gcacgtcctc gccgatttgc
     9301 gggtcgaaat ggcgcgtgaa cccaatggga agctggtcgg gattggacgc ggtcggtgcc
     9361 gggtccacga ggaagcgcca gcggcgcatg tgatccggtg cggcgaagcg ttggcgcgac
     9421 agcggaagct cgagattcac gaaccagcta taacgcactg cacccaccga ggcgccctgc
     9481 ggcatcgaag tgccctgcgc ggtgtagtag tagtgctcgc gcagcggcgt gttctgtccc
     9541 tgtccccagc cctggtccag ccagacgtac tcatcgacgt gttccagtgc gggaatgctg
     9601 tgtaccggcc acaacacgaa ccacgagtag agtcccgctc ccgccaccgc tccgagcacc
     9661 agcaggccgc ctagccactt cagaaatcgc cgcatcgcgt tactcccttg acgatctgac
     9721 gcatactaca aacaaggatc caacattcag gaagcgattg tgaaccggcc cggaagtgtt
     9781 gcaagtaccc gcacgtcgtg gacgtcgctg ctgatcctgt tagttagctg tctaccaatc
     9841 ccggttctcg gggattctcc ggccttcatc tcgctcgatc ccggtgcgcc ggtgcacgtc
     9901 gaagagctcg gtgatgcgct ggagctgctg gaggatcggg tcggcaacct cgacgtcgcc
     9961 gccgtcagta ccgcgcccgt cgacgccagg tttgtttccg ccacgctcga gtcgaccaat
    10021 atcggtttct ccccctcggc ctggtgggcg cgtgtgacgg tccgcaatcc gtcggacgcg
    10081 agccggctca tctaccttcg gcaggattac ccgctcatcg attcgctcga tctttatgag
    10141 ccctcggggg atggcggctg gcgggttcat tccacgggtg accgcaagcc attcggctca
    10201 cgcgacgtgg cgcacaaaga tttcctgttc ccggtgacgc ttccggccaa cagcgaccgc
    10261 actttctacc tgcgttatgc gtcgcaaggt ccggtggaca tcaacctttc gctgctcgat
    10321 cccaacgagg tcgcaggtga gttgagccgc gagcaagtcg cttacggctt gtatttcgga
    10381 tgcgtgctca tgttgctggt ctggagcggc ctggtgttcc tcgcggtgcg tgatggcgcg
    10441 ttccttgcgt atttcgccta cgttgcgacc ttcgggttgt acatgacggt caacacgggc
    10501 ttcgcgttcc agtacctctg gcccgacagt ccacgctggg ccaacaccgc gctcatcgtt
    10561 ctgctgaata ttgcgttgat cacggcgctg cagttttcac tgaccatcct gcgggcgcgc
    10621 gactacacac cgcgcctgtt cgtcgtggcg cgcgtgctac aggccatggc gctggtcgcc
    10681 atcacgctga tcccggtttt ctcgtacgcc gtgctggtca ggcccgtcac tttcctgatc
    10741 ctcgtctccg tctgcttcat gattgcgctg ggagtgatca gcctgctgct gggttcgcgt
    10801 cccgcgcgct tctacgtcat tgcctggggc gcattcctct gcggctccgt ggttttcctg
    10861 ctcaagaact tcgggttggt gccgcacacc ttcgtgacgc agcacagctg gcaggtgggc
    10921 gcgctcctcg aaatgatcct gctgtcgatg acgctctcca gtcgcatgag cgagctgcag
    10981 catcagagcc gcacggatcc gttgaccctg ctgggaaatc gccggcagtt cgacatcaac
    11041 cttccggccg aattcgcgcg ctccatggag caagggcaac cgctgtcgct cctggtggtc
    11101 gacatcgacc agttcaagct ctacaacgat ctgcatgggc acgcgcaggg tgacgaagcc
    11161 gtgaaggccg tggccgcggc gttgcgcaaa catgcgcgca agccgtatat cgcctgccgc
    11221 tacggcggcg acgaattctg cgccatcctt cccggcactt ccgaagcgtc ggccgcggtg
    11281 ctggcggaac gcctgcgcgc cgcggttcaa cattcgctca ccggtgaaga aaccgtcacg
    11341 gtcagcattg gttatgcgtg ccagacaggc ggccggttcg aaaacgccga ccagctgttc
    11401 gaagcggccg acgccgccct gtattccgcg aaggagcaag gccgcaacac cagcgtcgca
    11461 ttccaggggc gccgcagcaa cgatccgcct tctgtcaagg aagacgacgc gctcgacgtt
    11521 ccacgcgtgg gctgaagtct ggggtgatcc gagcgcctct tgcgctccct actacatctg
    11581 tcatagagtc gaccgcagtt gtagtcgtgg aggccgcgtg accgaaccga agctgagcgc
    11641 gctcgagctg cgcatcatgg gtgagctgtg gagccgcgga gcctgctcca ttcgcgagat
    11701 ccacgagggc gtggtcaaac gcgaccggcc cgcctacacc acggtgcaga ccatggtgta
    11761 ccggctcgaa gtcaaaggcg cggtgcgccg taccaagaag ataggcaacg cgcacgtgtt
    11821 cgaggccacc acttcgcgcg cttcggtgca gacgcgcctg ctcaaggagt tgctcgggct
    11881 gttcggcggc aacgcgcgcc cggtcatggc ccacctggtc gagatgggca agatttcgag
    11941 cgaagacatc gatgaagcgc agaagctgct gcgcgcgcac accgcgaaag agccggccgt
    12001 gaaggacgct cttccgaagg acaggggcag atgatcgccg cgctggtcaa ccacctgtgg
    12061 cagtccacgc ttttttgcgg cggcgcctgg ttgatcacgc tggcgttgcg cgccaacggc
    12121 gcggcgctgc gccactgggt ctggctgctg gcctcgctca agttcctggt gccgttctcg
    12181 ctgctgttct acgtgggctc gtacatcggc ctgcccgcag cgcgcaccgc tgacatccct
    12241 ccgttgatgc tcggagacgc tctgcaaagt gtctccctgc tggtctcgcc gagcgcgctg
    12301 ggggcgacag caacaggcgc gggttcgatc gcgctcgtca ttctgctggc ggtctggctc
    12361 ggcggcgccc ttctggtcgg cacgcgctgg ctgctcgcat ggcgcgccgc ggattcactg
    12421 gtaagggcag cgcggccggc gccgggcgca ttaccggatg cacgcatcac ggatgccgac
    12481 attgaaccgg ccgtggcgcg cgtttttcat ccagtggtgt tgctgccggc cgcgttgctc
    12541 gggcgactct cgcaacggga aatagacgcg gtactcgctc acgaacgcga acacatcgcg
    12601 cgccatgaca atctcaagga cagcgtccac cggctggtcg agacgttgtt ctggtttcac
    12661 ccggctgtgt ggtggatcgg ccggcagatg atcgaagaac gtgagcgcgc ctgcgacgaa
    12721 gccgtgctcg aggtcggcca cgatggcggt gattacgcct ccggaatact cgcggtgtgc
    12781 cgtcactgca gcgccggcgt ggcaggtact gccacggcca cctccggcaa tctcacgcaa
    12841 cgcgtgcgct acatcctggg tgatggacgc ccggcagccc tgggcgcggt caaggcaatc
    12901 tcgctgatgg tggccaccat cgccgccacc accgttccga tgttcgccgg ggccgtcgac
    12961 ggcacagcgc accggctcga gctgctcgcc atgaattcgc gcgcactggg gaccgccgaa
    13021 tttttcatct cgcccacggt gaacaatctc gattcacggc atcaggtcgt tgccggccgt
    13081 gacggagtcg tcatccgcaa cacctcgttg cgcgacctga tcgcgctctc atatggcgtg
    13141 gaacgctggc aggtgcgcgg caatggccaa tggctggatt cgctgcgcta tgacattcgc
    13201 gtcgtgacgc gcgacccggt gagcgagccc gacgaactcg acccctacgc gcttcgcgac
    13261 ctgaccgcca aactactcgc aacccgcttc gacctgcaaa tacacgtcaa ccgccaatgc
    13321 cagtcgcctt gcgggcgcta cgcacgggcg gccaatccca cgcagtagtc ccatgatcac
    13381 gggactggtc gatcatctgt ggcaatcgtg ctgctgctgc gggatcgcgt ggattttttc
    13441 cacgctgacg cgcgacaaca ccgccgtcgt gcacctgtgg ctctggcgaa tcgcggcact
    13501 gaagctgctg ttgccatttt cgctgctgtt cgcgctcggc gcgtggctgg gatttccctc
    13561 ggcgcacagt gcagatccgg cgccgccgga tttcgtgcgg gccatcagct tgctggcgcc
    13621 actcgcatcg ccagcgcgcg ttctcggatg gaccggcctg ccgctggcgc tcgccgccct
    13681 ggcggcattg gcggccgccg gcgcctgcgc gtactggtta cacgacaggc tacgcatcga
    13741 gcgcgagcgc gttatcgagg aagcggcgcg ccgcgaacgc gacatcgacg acatccagtc
    13801 gcggccgggc ttcatctgct ccgcgttgat gtcggcagtc gtactgtgcg ccgtctccat
    13861 accgatgctc gccggggcaa ttcacgatcg ccagcaacgc ctcgagcggc tgatcagcaa
    13921 ctcgcttgcg cttcgcgacg cgcccgtggt catggctccg gccgcgcgcg gcctgggaac
    13981 gcgctatcgc gtcgatgcgg acgcacacgg tgtctcggta cgcaatgcca acattcacga
    14041 tctcgtcgcc atcgtgtatg gcgtcagacg gcattcggtg attaccccgc agatgtattc
    14101 gggagtggaa tccgccagtc gctcctggtt gcactggccg cgatacgaca ttcgcatcca
    14161 ggcgcaggtg cccgatcctt ccgacttcga tccatacgcc ttgcgccaga acctcaccag
    14221 ggcgttgtcc gagcagttcg gcctcgagat ctacgtgaac ggcgactgcc agccgccttg
    14281 cggcaactac cacctcgcgc tggccaacga gcctctctag gccatcgctg cattgggcga
    14341 attttctggc cgcgttcaat tcgggggacc gcgcgacgct caccgcgtct ggaaaggatc
    14401 acgggccacc ggatttcctt cggccacaat tcattgacca gactctggag atgaaccgga
    14461 cttctggcgg ctacgacctg ctcgaggtgg gattcctctc ggcggatcca ccggagcgct
    14521 tccgcccgaa ggaatgacgg ccgttcgcgc aggctcctgc cggtgccggt tagcgtcgag
    14581 gccgccgatc aggtcgagcg ggctgcgccg cgcgactgcg gtttcgctaa ctaccctgca
    14641 ttctcacgcc taaccaccct tagtggattc acctgccacc agaggtggcc agttcgatga
    14701 agttgtgtgc gataccgaac agacctgcgc gccgcgttga ggtgagatcg tgcctggggt
    14761 cgctttgctg cggacgtgct cgaatgccta acaccagaag aattctcctt gttcaagaga
    14821 atgccgcgga cgcacgcgcc gtcagggacg ctctgacaaa ctcgggcccg tacgcactca
    14881 gcgtcgagtg ggtgaagaga tgctctcaag ccgtggaatt gctccgcggc gatgagacac
    14941 gaggcgcaga agggtttgca gcagtcctgg tcgatctgtt cctgtccgac agcagcggcc
    15001 tggatacctt cgaccggctt ttcaaagcgg cgccgcagct tccgatactg gtgctcagca
    15061 attcgcagaa tgaacccatc gcccggcagg cagtgcagcg gggcgcgcag gactacctgc
    15121 tcaagggccg cctcgacaac tacctgctgc cgaaagccct cggcagcatg atcgacagag
    15181 ctgcccacgc ggaggccctg ttcgaagaga aggaacgcgc gcaggtgacc ctgaactcca
    15241 ttggcgatgc ggtcctgagt tgcgatgtgg ccagccgtgt gacctatctg aacaaggtcg
    15301 cggaacagtt gacgggctgg acgggcgaaa aggctgcggg acgcccggtc gaagacgtgt
    15361 tccacatcct gaacgcctca acgcgcgcga cgattcctaa tccgatggcc ctggccatca
    15421 gcgagaacag gacggtgctg ctcgcgccga acagtattct gctcaggcac gacggtgtcg
    15481 aagccgccat cgaggattcc gccgcgccaa tccacgaccg ccatgggcgg gtcaccggcg
    15541 ccgtgatggt atttcacgac gtgagcagca cgcgcgcgct gtccctcaac ctggcccacc
    15601 aggcccagca tgacagcctc accgacctgc ccaacaggat gctgctggat gacaggctgg
    15661 gtcactcgat ggtggttgca cgccgccacg gcgtgaagct cgccgtcctg tacctggacc
    15721 tggaccgctt caagaaggtc aacgactcgg tcggccacga catcggcgac aagctcctgc
    15781 agatcgtcgc gcggcgactg ctgggctgcg tgcgcggctc ggacaccgtg agccggctgg
    15841 gaggcgatga attcgtggtc ctgctttcgg aagtggtggc gggccaggat gcggccatca
    15901 gcgcggagaa aatcctgcga gcgctgagcg gaccctttga catcgacggc catcatctgc
    15961 acgtctcggc cagcatcgga atcgctatct acccggaaga tggcgacgac gccgtatccc
    16021 tgctgaagca tgcggacttc gcgatgtacc atgcgaagga ttgcgggcgt aacaacttcc
    16081 agtttttccg cgaagacatg aatgtccgtg cgctggaacg tcagtcgatc gaagacggcc
    16141 tgcgcctggc gttggagcgc gacgaatttg tgctctacta ccagccaatc atggatttat
    16201 ggagcgggtc acctacgggg gtggaggcgc tccttcgctg gcgccacccg catcgcggcc
    16261 tgctttttcc gcagcagttc gttccgattg cggaggaatg cgggctgatc ctgccaatcg
    16321 gtcgctgggt cctgcgcgaa gcctgccgtc aacttcgcac ctggcaggac gagggtctgg
    16381 ctccactgcg tatcgccgtc aacgtctcgg cggtcgaatt gcgccacaag gatttcgtcg
    16441 agggcgtgcg ccaatgcctt tccgactgtg ggctcgctgc ccagcatctg gaactcgagc
    16501 tgaccgaaac ctttctcatg caggactccg cgactaccgc gctggtgctg aaggcgctca
    16561 aggacctggg cgtccagctt gcgctcgatg actttggcac cggttattcg agcctgagtc
    16621 acctgaaggg ctttccgatc gatacgctga aaatagatca ggcgttcgtc agcgaactcg
    16681 attcaaatcc caatgacgcg agcattgttc gcgccgtgat aggtctcggg cgaaacctca
    16741 acattcgcgt ggtggccgag ggagtggaaa ccgcggcgca gctggcgcga ctccaggaac
    16801 agcaatgccc ggaagggcaa ggctactttt tcagccggcc ggtgatcgcc gacgaactcc
    16861 cgcgcctgtt actcaccgcg ccaccgggtt ttccggacct gcacgacgga gtcggcgacg
    16921 cattcaatgg cgggcccgtc gttccaggac ccacggacgc accaccgcgg cacgtttcac
    16981 gaagctctcg caactgacag atgactccgg ctctaactat tggcaagcag gtccagctgc
    17041 ccacgccttg ccacgcgtcg atcgaagaga ccccacgcac cctcaccccc atgccatgcg
    17101 ccacccgtcg cggccttcgc gtactcggtg gcgcgggtct cgaagaagtt ggcgtgctcg
    17161 accccgttca gcatggcggt gagccagggc agcggatgct ccttcacgcc gtagacctgc
    17221 ggcaggccta actgcttgag gcgccagtcg gcgatgtagc ggatgtaggc cttgatctcg
    17281 gcggccgaga gcccttcgat ggggcccatc tcgaatgcga gatcgacgaa gcgatcctcg
    17341 agtttcacca ccgtgcgcgc gcaatcgacg atgtcgtcct tgacggcctg cgtcagcgcg
    17401 cccgtctcct gcgcgaaggt gtggaacagc cggatgatgc cttcgcaatg cagggtctcg
    17461 tcgcgcacgc tccacgagac cacctgcccc atgccacgca tcttgttgtg gcgcgggaag
    17521 ttgagcagga tcgcgaagct ggcgaacagt tgcagtcctt cggtgaagcc gccgaacacc
    17581 gcgagcgtgc gcagcgtatc ggcttcggtg tcgacaccga acccctgcag gtaatcgtgc
    17641 ttgtcacgca tggccgcgta gtccatgaat gccgtgaatt cgctgtccgg catgccgagc
    17701 gtctccagca acagtgcata ggctgcgata tgcaccgtct ccatgttcgc gaacgccgcc
    17761 agcatcatct tgatctccgt ggggcggaac acgcgcgcgt agcgctccat gtagttgtcc
    17821 tgcacctcga cgtccgcctg cgtgaagaag cggaacacct gggtcagcag attccgttcc
    17881 gcatcggtga tgcggttgtt ccagtccttg caatcttcgc cgagcggaat ttcttcgggc
    17941 atccagtgga tctgctgctg gcggcgccaa tactcgattg cccagggata acgaaagggc
    18001 ttgtagctgc gcgatggcgt gagcaatccc gggcgcgtga tcaactcgat ctggcttact
    18061 gacatgacag gcactcgtcg tatttggctt cgtcggtatc ggaggtgagc ggccctttca
    18121 gaggttttgg cggccacgac cgcgtgttgt ccgcttcgac accagcgaac ccggcgcgct
    18181 gaacactctt gctgcggcag tagtacagcg atttcacgcc gcgctcccac gccgtccagt
    18241 gcagcatgag cagatcccac ttgtggatgt cgctgcgcag atagaggttg agcgactgcc
    18301 cctggcagat gagcggcgcg cgatcggcag ccagctcgat gacccagcgc tggtcgattt
    18361 cgaatgccgt gcggaacacc gacttctcgt cgtcggtcag gccctcgaga tgttgaacgg
    18421 aaccctcgtg cgcgaggatc gactgccagg tgtcttcggt gttcagaccc cttgcgccca
    18481 acaccttctg cagcgccgga ttgcgcacgc tgaacgaacc ggacagcgtc ttgtgcgtat
    18541 agatattcgc cgggatcggc tcgatgcagg cgctggtacc gccgcaaatc accgagatgc
    18601 tggccgtcgg cgcgatcgcg agcttgtgtg agaagcgttc catcgtgccg cgctcggcag
    18661 cgtcgggaca ggcgccgcgt tcttcggcga gtacgcgcga cgcggcgtcg gcctcgcgtc
    18721 gcaggtgctt gaacatgcgc aggttcgccg cgcgcgccag cgcgctttca aacggaatgc
    18781 cgttgttctg cagccaggag tgaaagccca tcgcgccgag gccgaccgag cgctcgcgca
    18841 tcgccgaata cttcgccgtg tccatcgaag ccggtgcatg gtcgatgaac tcctgcagca
    18901 cgttgtccag catgcgcagc acgtcttcga tgaagcgcgg ctccgtgcac cactgctccc
    18961 aggcctcgaa attgaccgat gacaggcagc acaccgcggt gcgggcgcgg ccgaggtgat
    19021 cgacgcccgt gggcagcagg atctcgctgc acaggttcga ctggcgcacc ttgagcccaa
    19081 gttcgcgctg atgaggtgcc agctgattgt ttgcggtgtc gctgaacagc aggtacggct
    19141 cgccggtctg cagacgtgtc tcgagaattt tctgccacag gtggcgcgcc gacagcgtgc
    19201 gcaactcctt gccgtctttg gggctgcgca gcgcgaacgg cagatcgtcg cgcaccgcct
    19261 gcatgaattc gtcggtgatg cagatgccgt ggtgcaggtt gaggctcttg cgattgaaat
    19321 cgcccgaggg tttgcggatt tcgatgaact cttcgatctc gggatgaaag acatcgagat
    19381 acacggctgc cgatccgcgg cgcagcgagc cctgcgagat cgcgagcgtg agcgaatcca
    19441 tgacgcgcac gaacggaatg attcccgagg tctggccaca ccccttcacg gtctcgccga
    19501 tggaccgcac accgccccag taagtcccga ttccgccgcc attcgacgcc agccagacgt
    19561 tttcg
//
LOCUS       DASQJS010000249         4664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3915057, whole genome shotgun sequence.
ACCESSION   DASQJS010000249 DASQJS010000000
VERSION     DASQJS010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3915057"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..2119
                     /gene="flhA"
                     /locus_tag="VEW08_10090"
     CDS             59..2119
                     /gene="flhA"
                     /locus_tag="VEW08_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HYJ41132.1"
                     /translation="MSPLMRLGLGVPIGLISILAMMILPLPPLVLDVLFTFNIALSLV
                     IVMAVFNVRRPLDFAIFPTVLLLVTILRLALNVASTRVVLMHGHEGSHAAGKVIAAFG
                     EFVIGGNFAVGAVVFIVLTIINFVVVTKGAGRVSEVSARFALDAMPGKQMAIDADLNA
                     GLITQDEARQRRAEVRAESDFYGSMDGASKFVRGDAVAGILIMVINIIGGLLIGTMMH
                     DLPMGEATKTYTLLTIGDGLVAQIPGLMLSVAVAIIVTRVTREQDVGGELQKQLFGQP
                     KALGVAAAVLGIMGLIPGMPNLVFLAIGGLCGFAAWRLVKKREAEAAAPVLAPEPPPP
                     PPSTESQELTWEDVRPVDAVGLEVGYRLVPLVDRAKGGDLLARIRGIRRKLTQELGFL
                     VQAVHIRDNLELGPNTYRIRLAGVAIGESVVHPDRELAINPGRVFGPVNGLATRDPAF
                     GMEAVWIEPNLREHAQSLGYTVVDSATVIATHLSHLIQSHAHELFGHEEAEQLLKSLA
                     KTAPKLVDELIPKVLPLAVVVRVMQGLLAERVPIRNVRAIVEALAEAGPRSQDPAVLQ
                     AQVRVALGRQIVQDIVGLAEELPVITLEPELERLLSNGLINSAAGPGLEPGLAERLQR
                     GLSEAAQRQERGGEPAVLLTPPALRTALARFFRVSVPGLHVLAWNEVPDNRKVRMVTA
                     VGAR"
     gene            2134..3384
                     /gene="flhF"
                     /locus_tag="VEW08_10095"
     CDS             2134..3384
                     /gene="flhF"
                     /locus_tag="VEW08_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HYJ41133.1"
                     /translation="MRIQRYFAKDMRSALAQVREAMGPEAVILSTGTVGDQVEVVAAI
                     DVEIAIAHAAPQGAVSSTSGTSAETLQRAAPRRASSQLSEALVPKPVQAAAPTPATVP
                     APQAPEQNNELANEVKDMRRMLEAQLATLAWNDMSRRSPLQAAMLKELAQLGITQDLA
                     SSLARKIPTNLNFSAARRFALATIARTVQVTGDRWLETGGRIAFAGPAGAGKTTLLAK
                     LAARWVLRHGPRRVALVSADAVRIGAHEQMHMLGRLLGVTAHTVYDVAELPELLDALS
                     GQQLVLIDTAGASPRDPDLARRLRLLDQSKSQIETSLVIPASIQAGAIEEAMKLFAVA
                     RPSSCVITKIDEAVSLGGVLSSLVRHKLPVAYISDGQRVPEDLQPARAHSLVSRAVEI
                     ASRNGATADDEVMQRRITGALHGR"
     gene            3374..4255
                     /locus_tag="VEW08_10100"
     CDS             3374..4255
                     /locus_tag="VEW08_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003184001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HYJ41134.1"
                     /translation="MGVDKAQPKLDGVMRANQSPVQVIAVTGGKGGVGKTTIAVNLAA
                     SLAGRGKQVMLLDGDLGLANVDVFLGLTPRLTLADVLAGNCTLEEILLDAPQGFKVVP
                     AASGIAQLAELDTLTHVGLVRAFGDITSKVDIMVIDTAPGIAGSVLQFSQAAQQVLVV
                     LCDEPASLTDAYALIKILSRDHGVKQFRVVVNQVRGRGMGQALFQRFERVATRFLSVD
                     LDYVGEIPEDAFLRRSIREQRPVVSAYPSAPASVALKTLAQRADNWPVADGPRGNVEF
                     FVERLIRRPAVRLEVVR"
     gene            4255..>4664
                     /locus_tag="VEW08_10105"
     CDS             4255..>4664
                     /locus_tag="VEW08_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ41135.1"
                     /translation="MGVSAYSQRPSARETEALVMRHAELVKRIAYHLAGRLPASVEVD
                     DLIQAGMLGLLEAAANYSEGRGASFETYAGIRIRGAMLDGLRKLDWAPRSVHRKARAV
                     AKAIRELESEYGREARDVEVAERMGMKLSEYHRIV"
BASE COUNT          809 a         1514 c         1529 g          812 t
ORIGIN      
        1 acctccgccg ccccccgtca tccattaaaa cgccatggca gaaaacaaca gttccgcgat
       61 gagtccgctg atgcgcctgg gtctcggcgt gcccatcggc ctcatctcga ttctcgcgat
      121 gatgatcctg ccgctgccgc cgctggtgct cgacgtgttg ttcacgttca acatcgcgct
      181 gtcgctggtg atcgtcatgg cggtgttcaa cgtcaggcgg ccactcgact ttgcgatttt
      241 cccgaccgtg ctgctgctcg tgaccatcct gcgcctggcg ctcaacgtcg cctcgacgcg
      301 cgtggtgctc atgcatggcc acgagggctc gcacgccgcc ggcaaggtca ttgccgcgtt
      361 cggggagttc gtcatcggcg gcaattttgc ggtgggcgcc gtggtgttca tcgtcctcac
      421 catcatcaac ttcgtggtgg tgaccaaggg cgcgggtcgc gtgtcggaag tgagcgcacg
      481 tttcgcgttg gacgcgatgc ccggcaagca aatggccatc gacgccgatc tcaacgccgg
      541 cctcatcaca caggacgaag cccgccagcg ccgcgccgaa gtgcgtgccg aatccgattt
      601 ttacggctcg atggacggcg ccagcaagtt cgtgcgcggc gatgccgtcg ccggcatcct
      661 catcatggtc atcaacatca tcggcgggct gctcatcggc acgatgatgc atgacctgcc
      721 catgggcgaa gccaccaaga cctacacgct gctcaccatc ggtgacggtc tcgtggcgca
      781 gattcccgga ctcatgttgt cggtggccgt cgccatcatc gtcacgcgcg tcacgcgcga
      841 gcaggacgtg ggcggcgaac tacagaagca gttgttcggc cagcccaagg cgcttggcgt
      901 agccgccgcg gtgctcggca tcatgggact catccccggc atgcccaacc tggtattcct
      961 cgcgatcggc ggattgtgcg gattcgccgc ctggcgtctc gtgaaaaaac gcgaggcgga
     1021 agccgcggcg cccgtgctgg cgccggaacc gccaccgccg ccgccctcga cggaaagcca
     1081 ggaactgacc tgggaagacg tgcgtccggt agacgccgtg ggcctcgaag tgggctatcg
     1141 cctcgtgccg ctggtggatc gcgccaaggg cggcgacttg ctggcgcgca tccgcgggat
     1201 tcgccgcaaa ctcacgcagg agctcggctt cctggtgcag gccgtgcaca tccgcgacaa
     1261 cctggaactc ggacctaaca cataccgcat ccggttggcg ggcgtggcga taggcgaaag
     1321 cgtggtgcat ccggatcgcg agctcgccat caatccgggc cgcgtgttcg gtccggtgaa
     1381 cgggctggcg acgcgtgacc cggcattcgg catggaagca gtgtggatcg agccgaacct
     1441 gcgcgagcac gcgcagtcgc tcggctacac ggtggtcgat tccgcgacgg tcatcgccac
     1501 gcatctgtcg cacctcatcc agtcgcacgc gcacgaattg ttcggccacg aagaggccga
     1561 gcagctgctc aagagcctgg ccaagaccgc gccgaagctg gtcgacgagc tgataccgaa
     1621 agtgttgccg ctcgccgtgg tcgtgcgcgt catgcagggg ttactcgcag aacgcgtgcc
     1681 catccgtaac gtgcgcgcca tcgtcgaagc attggcggaa gccggtccgc gttcacagga
     1741 tcccgccgtg ctgcaggcgc aggtcagggt tgccctgggg cggcagatcg tccaggacat
     1801 cgtcggtctc gccgaagagt tgcccgtcat cacacttgag ccggaactcg agcgcctatt
     1861 gtcaaacgga ctcattaatt ccgccgccgg acctggtttg gagcctgggc tggcagagcg
     1921 tttgcaacgt ggattgtcag aggccgcaca acgtcaagaa cgtggcggtg aaccggcggt
     1981 gttgttgacg ccaccggcgc tgcgcacggc actggcgcgg ttcttcagag tcagcgtgcc
     2041 ggggctgcac gtgctcgcct ggaacgaagt gcctgacaac cgaaaggtcc gcatggtgac
     2101 cgcggtcggc gcgagataag agggcaatag agaatgagaa ttcaaagata cttcgccaag
     2161 gacatgcgca gcgcgctcgc gcaggtgcgc gaggccatgg gccccgaggc ggtcatcctg
     2221 tccacgggca ccgtcggaga ccaggtcgaa gtggtcgccg cgatcgacgt ggaaatcgcg
     2281 atcgctcacg cggcgccgca aggagccgtg tcctccacgt cgggtacatc ggcggagacc
     2341 ttgcagcgtg cggctccgcg ccgtgcttca tcgcagttgt ccgaggcact ggtgccaaag
     2401 cctgtgcagg ctgccgcacc aacgcccgca actgtgcccg cgccgcaggc gccagagcag
     2461 aacaacgagc tcgccaacga agtcaaagac atgcgccgca tgctcgaagc gcagcttgcg
     2521 acacttgcgt ggaatgacat gtcgcggcgc tcgccgctgc aggccgccat gctcaaggag
     2581 ctcgcgcagc tcggcatcac gcaggatctc gccagctcgc tggctcgcaa gattcccacc
     2641 aacctgaatt tttcggcggc gcgccgtttc gcgctcgcca ccatcgcgcg caccgtgcag
     2701 gtcacgggcg accgctggct cgagaccggc ggccgcatcg ccttcgccgg ccctgccggc
     2761 gccggcaaga ccactttgtt agccaagctc gccgcccgct gggtgttgcg tcacggtccg
     2821 cggcgcgtcg cgctggtgtc ggccgacgcg gtgcgcatcg gcgcgcatga acagatgcac
     2881 atgctgggtc gcctgctcgg cgtcacggca cacacggtgt acgacgtcgc cgaattgccg
     2941 gaattgctgg acgctttgtc cggccagcaa ctcgtgttga tcgacaccgc gggcgccagc
     3001 ccgcgcgacc cggatctcgc gcgccgcctg cgactgctgg accagtcgaa atcgcagatc
     3061 gaaacctcgt tggtgattcc ggccagcatc caggcgggcg ccatcgaaga ggctatgaaa
     3121 ctgttcgcgg tggcgcgtcc gagctcctgc gtgatcacca agattgatga agccgtgagc
     3181 ctgggtggcg tgttgtcgtc actggtccgg cacaagttgc ccgttgcgta catcagcgac
     3241 ggccagcgtg tgcccgagga cctgcagccg gcacgcgcgc attcgctcgt gagccgcgcg
     3301 gtggagatcg ccagccgcaa cggcgcgacg gcggacgacg aagtcatgca gcgacgcatc
     3361 acgggagcgc tccatgggcg ttgataaggc tcaaccgaag ctcgacggcg tcatgcgcgc
     3421 gaatcagtca cccgtccagg tcattgccgt caccggtggc aagggtggag tgggaaaaac
     3481 aaccatcgcc gtgaatctcg cggcctcgct cgccggtcgc ggcaagcagg tcatgctcct
     3541 ggacggagac ctgggtctgg ccaatgtgga tgtattcctg ggcctgactc caaggctcac
     3601 gctcgcggac gtactcgcgg gcaactgcac actcgaggag atcctgctgg atgctccgca
     3661 aggtttcaaa gtcgttccgg ccgcctcggg catcgcccag ctcgccgagc tcgacacgct
     3721 cactcacgtg ggcctggtgc gcgccttcgg cgacatcact tccaaagtcg acatcatggt
     3781 catcgacacg gcgccgggaa ttgccggcag cgtgctgcag ttctcccagg ccgcgcagca
     3841 ggtgctggtg gttttatgtg atgaaccggc ctcgctgacc gatgcctacg cactcatcaa
     3901 aatccttagt cgcgaccatg gggtcaagca gttccgcgtg gtcgtgaacc aggttcgcgg
     3961 ccgcggcatg gggcaggccc tgttccagcg tttcgaacgt gtggccaccc gattcctgtc
     4021 ggtggacctc gattacgtcg gcgagatccc cgaagacgcg ttcctgcgtc gctcaattcg
     4081 tgaacagcgt cccgttgtca gcgcctaccc cagcgccccg gcctcggtgg cactcaagac
     4141 gctggcgcag cgcgccgata actggcccgt ggcggacggt ccccgtggca acgtggagtt
     4201 ttttgtcgaa cgcctgatca ggcgtcccgc ggtgcgcctc gaggtcgtcc gatgatcggt
     4261 gtcagtgcct atagccaacg ccccagcgcg cgggaaaccg aagcgctggt gatgcgacat
     4321 gccgagctgg tcaagcgcat cgcctatcac ctggcggggc gcttgcctgc gtcggtggaa
     4381 gtggacgatt tgatccaggc cggaatgctc ggcctgctgg aagccgccgc gaactattca
     4441 gaagggcgtg gcgcgagttt cgagacctac gcgggtatac gaatccgcgg cgcgatgctc
     4501 gacggactgc gcaagctcga ctgggcgccg cggtcggtgc atcgcaaggc cagggcggtc
     4561 gcgaaggcga tccgcgagtt ggaatctgaa tacggccgcg aagcgcgcga cgtggaagtg
     4621 gccgagcgca tgggcatgaa gctcagcgag taccaccgca tcgt
//
LOCUS       DASQJS010000250         6527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3597385, whole genome shotgun sequence.
ACCESSION   DASQJS010000250 DASQJS010000000
VERSION     DASQJS010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3597385"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VEW08_10110"
     CDS             <1..1065
                     /locus_tag="VEW08_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HYJ41136.1"
                     /translation="YKMIKFSIAIQRGCFGGCTFCSITEHEGRIIQNRSEGSVLREIE
                     KIRDEVPGFTGVISDLGGPTANMYRIACKSREIESACRKPSCVYPGICPNLNTDHSAL
                     INLYRKARALPGVKKILIASGVRYDLAIESPEYVKELATHHVGGYLKIAPEAIGEGPL
                     SKMMKPGVGAYHKFKELFDKFSKLAGKEQYLIPYFIAAHPGTTDKDMLDLALWLKQNG
                     FRADQVQAFLPGPMATATAMYHTLKNPLKRVTRDSEEVVIPKGIKQRRLHKAFLRYHD
                     PNNWPVLREALRKMGRADLIGNGKQQLVPGYQPAGTGDAAEGKRGGSQNRPFKTQHTG
                     LPRVPSLGNAKKNRRDPRSN"
     gene            1108..1728
                     /locus_tag="VEW08_10115"
     CDS             1108..1728
                     /locus_tag="VEW08_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41137.1"
                     /translation="MKARVSLVLALMAGTFLITGCGTSIYSVKIPFSEKPDAVFADAP
                     HVTIEDTRSEEDRTTHVGGGLSRCIRWYGDDTYQPPKLVYLDHLIAERVPPATQVSIR
                     LDTFDTIEYCDNTADRGAAAAVGGVTGVFIPATTRPGGDSVHVQLVGEINGVAFDVSR
                     AFDYDGLPYKFGEMPAANITYRELLRKMMSEIADEIVAKLQVARAP"
     gene            complement(1792..3636)
                     /locus_tag="VEW08_10120"
     CDS             complement(1792..3636)
                     /locus_tag="VEW08_10120"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYJ41138.1"
                     /translation="MCTFMTYLRAATLAVLAGVGVVQAAELEGLWKAKGRFGPDARGT
                     LTIQKDGATYTADMLGRRVPVRLEAGELGFELPNRLGRFRGKLEAQNIRGHWFRFGTP
                     VNGSGSTAPVVLSPVLLKPAGPNHWRGEVAPLQDDFTFFLLLSEKRADGSFGAVLRNP
                     EFDFGTQQGVERFVRDGNLVKLVGKRREKEQIVSVGTYDDDNQVITLIFPGRGGSYDF
                     RRDGDDSDFYPRGKNPARYSYRPPPARDDGWTTSTLDAANIDRAAMERFVQTIVERRM
                     DSTDAPQIHGVLIARHGKLVLEEYFHGEHRDKPHMTRSASKSVTAVLVGAAIHAGAPL
                     KLSSPVYQIMNGGKFPPDLEPQKRTMTLEHLLTMSSGFFCDDNNDNAPGNEETMWDQA
                     EEPDFYRYTMKVPMVAPPGENAVYCSASPNLALGMVGRATRELPIYSFDRLIAGPMKI
                     GNYAWPLDPAGNPYGGGGAQFLPRDFMKFGQLMLNGGTWEGHRILSRDFVKHATAPLY
                     NLAHIRYGYNWWSEDFPYKDRNVHAFMALGAGGQVVTVVPELDLVIAFYAGNYSSRSQ
                     RDLGHTFVPRYLLPAVREPGDNKSAPVVDRDYTSPYGRSTDGSRVPQK"
     gene            3765..4604
                     /locus_tag="VEW08_10125"
     CDS             3765..4604
                     /locus_tag="VEW08_10125"
                     /inference="COORDINATES: protein motif:HMM:NF041645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HYJ41139.1"
                     /translation="MKTPPGLQPLIDDGVIDEVIRSLKSGKEATVYTVRSGSQIRCAK
                     VYRDMGQRSFQKRAQYQEGRKVRGSRQARAMAKSTRYGRKEAESAWKNTEVDALYKLV
                     AAGVRVPRPFGYFNDTLVMELVTDASGNPAPRLGELDLTPEVAREYHTFLIRQIVRML
                     SIGLIHGDLSEFNVLVDAAGPVIIDLPQAVNAAGNNAAFAMLERDVNNIRGTLGRFAP
                     ELLETEFAREIWALFEAGDLQAESVLTGVFARPEGVTDPDAVMVVIDDAREENIRREL
                     GRQ"
     gene            4771..>6527
                     /locus_tag="VEW08_10130"
     CDS             4771..>6527
                     /locus_tag="VEW08_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ41140.1"
                     /translation="MNCRFWPPAVGALLVAACVQGPSVPDGAVLLDEEVTLARGPNVD
                     SAQREFEVQGGSVVVALVDEQLTDIKLQLAVDDSSGKTPVSVEVENNLRGAGVEIAAA
                     TVLRGSRIRVTLTGQHDATKPGHVRLRLRRFDADAKQSKLAAVLEGFRAWSAATNADY
                     RVDAITGSALADMDRAIERLESAQGDAALAAQARLIKSNMFYYFHVDWREARAEAQRA
                     AAAFRSMPAPDRLGEARAQFAEARALVKISANSKSVDPTAGEAAKLARESLTTLGSAT
                     SVFGPVERAGAIGALGHIDFGTAMLDAAEKRFEESRAMYEAAGYSAGELEMRCNLAAV
                     PLERGQWRVAAEAFVELLPELDKISNPELRAMMYLGAARGQSFSGRTDQAAENQLKAL
                     ALAREYKLRAQEASALHGLAHVYQNRGDTLQARAFFAEVLRIARDGDDVMEYVWALAS
                     AGTVARDDGDFARAIEMHKEAVSRASNPIGLVRTLRELGQDYYFARDYPAAIVEFRKS
                     LAVDLHDPTHHAYSDVKRNLAQALIDNGERTPATFKEAAKLLDETLQSSLRVSDKQGV
                     LGAHRVRAQLFAEQGKPAEA"
BASE COUNT         1253 a         2038 c         2100 g         1136 t
ORIGIN      
        1 tacaagatga tcaagttctc catcgccatc cagcgtggat gtttcggcgg atgcacgttc
       61 tgcagcatca ccgagcacga aggacgcatc atccagaacc gcagcgaagg cagcgtgctg
      121 cgcgagatcg agaagatccg cgacgaagtg cccggcttca ccggcgtcat cagcgatctc
      181 ggtggcccca ccgccaacat gtatcgcatc gcgtgcaagt ccagggagat cgagagcgcc
      241 tgtcgcaagc cgagctgcgt gtatcccggc atctgcccca atctcaacac cgaccacagc
      301 gcgctgatca acctctatcg caaggcgcgc gcgctgccgg gcgtgaagaa gatcctgatc
      361 gcgagcggtg tgcgctacga cctcgccatc gagagccccg aatacgtgaa ggagttggcc
      421 acgcaccacg tgggcggcta tctcaagatt gcgcccgaag ccatcggcga agggccgctg
      481 tccaagatga tgaagcccgg cgtcggcgcg tatcacaagt tcaaggagct gttcgacaag
      541 ttcagcaagc tcgccggcaa agagcagtac ctgattccgt acttcatcgc cgcgcatccc
      601 ggcaccaccg acaaggacat gctcgacctc gcgctgtggc tcaagcagaa cggtttccgt
      661 gcggaccagg tgcaggcgtt cctgcccggg cccatggcca ctgccactgc gatgtatcac
      721 acgctcaaga atcccttgaa gcgagtgacg cgggacagtg aagaggtggt gattccgaaa
      781 gggatcaagc agcgcaggct gcacaaggcg ttcctgcggt atcacgatcc gaacaattgg
      841 ccggtgctgc gcgaggcgtt gcggaagatg ggtcgtgccg atctcatcgg gaacggcaag
      901 cagcagttag tgccggggta tcaacccgcg ggcactggag atgcggcgga agggaagaga
      961 ggcggcagcc aaaatcggcc gttcaagacg cagcacaccg ggttgccgcg cgtgccgtca
     1021 ctcggaaacg caaagaaaaa ccggcgcgac ccccgctcaa actagctatc atccgcgcag
     1081 ttagtcgact gtttccctgg gggagagatg aaggcgcgcg tttcgctggt gctggcattg
     1141 atggcgggta cattcctcat caccggctgc ggcacgtcca tctacagcgt gaaaatccca
     1201 ttcagcgaga agcccgatgc ggtcttcgcc gacgcgccgc acgtgacgat tgaagacacg
     1261 cgcagcgaag aagatcgcac tacgcacgtg ggcgggggcc tcagcaggtg catacggtgg
     1321 tacggcgacg atacctacca gccgcccaag ctcgtgtacc tggatcacct gatcgctgag
     1381 cgtgttccac ccgcgacgca ggtcagcatc cgtctggaca cgttcgacac catcgaatac
     1441 tgcgacaaca cggcggatcg tggggcagcc gcggccgtgg gtggtgtgac cggtgtcttc
     1501 atccccgcaa ccacgagacc aggcggcgac agcgtgcacg tgcagcttgt cggagaaatc
     1561 aacggtgtgg ccttcgatgt ctcgcgcgcc ttcgactacg acgggctgcc ctacaagttc
     1621 ggcgagatgc cggcggccaa catcacctac cgcgagttgc tgcgcaagat gatgagcgag
     1681 attgcggacg agatcgtcgc gaagctgcaa gtagcacgcg cgccctaaca acaagctctg
     1741 caatttactt caactggcgc ggcacgacat tccaacgccc cccattgccg cctatttctg
     1801 cggcacccga ctcccatccg tcgaccgccc atacggactg gtgtaatccc ggtcgacgac
     1861 cggcgcactc ttgttatctc ccggttcacg cacggcgggc agcaggtagc gaggaacgaa
     1921 ggtgtgtccg agatctcgct gactccggct gctgtagttg ccggcataga acgcgatgac
     1981 caggtcgagc tccggcacca ccgtcaccac ctggccgccg gcgccgagcg ccatgaacgc
     2041 atgcacgttg cggtccttgt atgggaagtc ctcgctccac cagttatagc cgtagcggat
     2101 gtgcgccagg ttgtagagcg gcgcggtggc gtgcttcaca aagtcgcggc tgagtatccg
     2161 gtggccttcc cacgtcccgc cattgagcat gagctggccg aacttcatga aatcccgcgg
     2221 caggaattgc gccccgccgc cgccataagg attcccggcc ggatcaagcg gccaggcata
     2281 gttgccgatc ttcatcggtc cggcgatcaa ccggtcgaag ctgtagatag gaagctctcg
     2341 cgtcgcgcgt cccaccatgc ccagggccag gttcgggctg gcactgcagt agacagcgtt
     2401 ttctcccggc ggtgcgacca tcgggacctt catcgtgtag cgatagaagt ccggctcctc
     2461 ggcctgatcc cacatcgtct cttcgttgcc gggcgcgttg tcgttgttgt catcgcaaaa
     2521 gaacccggac gacatcgtca gcaaatgctc cagcgtcatg gtccgcttct gcggttcgag
     2581 gtccggcggg aacttgccgc cgttcatgat ctggtaaacc gggctggaaa gtttgagtgg
     2641 agcacccgcg tgaatcgccg cgccgacgag cacggcggtg acgctcttgg acgccgaacg
     2701 cgtcatgtgc ggcttgtcgc gatgctcgcc gtggaaatat tcctcgagca ccagcttgcc
     2761 gtgccgcgcg atcagcacgc cgtgtatctg cggcgcatcc gtcgaatcca tgcgcctctc
     2821 gacgatggtt tggacgaatc tctccatggc agcgcggtcg atgttcgcgg catcgagagt
     2881 cgatgtggtc cagccgtcgt cgcgggccgg cggcggccgg taggaatagc gggcgggatt
     2941 cttgccgcgc ggataaaaat cactgtcgtc gccgtcgcgc cggaaatcgt agctgccgcc
     3001 tcgccccggg aagatgagcg tgatcacctg gttgtcgtca tcgtaagttc cgacactcac
     3061 gatctgctcc ttctcgcgcc gcttgcccac cagcttcacc aggttgccat cccggacgaa
     3121 cctctccacg ccttgctgcg taccgaagtc gaactcgggg ttgcgcagta ccgcaccaaa
     3181 cgatccgtcg gctcgctttt ccgacaacag aagaaagaag gtgaagtcgt cctggagcgg
     3241 ggcaacttcc ccgcgccaat ggttgggacc ggccggtttg agaagcacgg gtgaaagcac
     3301 gactggcgcg gtactgccgc tgccgttcac gggagtgccg aaccggaacc aatggcctcg
     3361 gatgttctgc gcctcgagct tgccgcgaaa tctgccgagg cggttcggca gttcgaaacc
     3421 gagttcgccg gcttccaggc ggaccggcac ccggcggccc agcatgtcgg cggtgtaggt
     3481 ggccccgtcc ttctgaatgg tgagtgtgcc gcgtgcatcc gggccaaaac ggcccttcgc
     3541 tttccacaag ccctccagct ctgcagcctg gactacgccg acgccggcga gaacagcgag
     3601 ggtggccgcg cgcaggtacg tcatgaaagt gcacattgca gggggatgat gccgcaggtg
     3661 tccaggacgt ttcagtagga gcacgcgccg aggctattgg cgaaggcgaa gtgagaacag
     3721 acggctctct tgggaggccc gtgcctgcta ccatccgcgc ccccatgaaa acccctcccg
     3781 gcctgcagcc gctgatcgat gacggcgtca tcgacgaagt catccgttcc ctcaaatccg
     3841 gcaaggaagc gaccgtctac acggtgcgtt ccggctcgca gatccgttgc gccaaggttt
     3901 accgtgacat gggccagcgc agcttccaga agcgcgcgca ataccaggaa ggccgcaagg
     3961 tacgcggcag ccggcaggcg cgcgcgatgg ccaagagcac gcgttatggc cgcaaggaag
     4021 cggagtccgc ctggaagaac accgaagtcg atgcgctgta caaactagtg gcggcgggcg
     4081 tgcgcgtgcc gcgtcccttc ggctacttca atgacacact ggtcatggag ctggtgacgg
     4141 acgcgtcggg caatccggcg ccgcgccttg gcgaactcga tctgactccc gaagtggcgc
     4201 gcgaatacca caccttcctg atccgccaga tcgtgcgcat gttgagcatc ggcctcatcc
     4261 acggcgatct ctcggagttc aacgtgctgg tcgacgccgc tggcccggtc atcatcgacc
     4321 ttccacaggc agtgaacgcg gcgggcaaca acgcggcctt cgccatgctg gagcgcgacg
     4381 tgaacaacat ccgcggcacg ttgggccgct tcgcgccgga actgctggag acggaattcg
     4441 cgcgcgagat ctgggcgctg ttcgaagcgg gcgaccttca ggcggagagc gtgctcacgg
     4501 gtgtgttcgc ccggcccgaa ggcgtcaccg atccggatgc ggtgatggtc gtcatcgacg
     4561 atgcgcgcga agaaaacatc cgccgggagc tgggacggca gtagaggtct gttcgcaaca
     4621 gacagtcctg atggctggct ggaaagtgtc caaagatgtc ttgagacata cccgcccgac
     4681 aggctttcga ccgcggaatt ccgcgaagtc ctgcgccacg tcgcccgggt tgatctaagc
     4741 ttcccccggt cagggggatc taaaaatctt atgaattgca gattctggcc gccggctgtc
     4801 ggcgccctct tggttgccgc gtgcgtgcag ggtccgtccg ttcccgatgg cgcggtactt
     4861 ctcgatgagg aagtgaccct cgcgcgcggg ccgaacgtag actcggctca acgcgagttc
     4921 gaggtgcagg gtggttcggt cgtcgtcgct ctggtcgacg agcaactgac cgacatcaag
     4981 ctacagcttg cggtcgacga ttccagcgga aagaccccgg tgtccgtcga ggtcgaaaac
     5041 aatctccgcg gcgcgggcgt ggaaatcgcg gccgccacgg tactgcgcgg ctctcgaatc
     5101 cgggtgacgc tcaccggtca gcatgatgca accaagcccg ggcacgtgcg cttgcgcctg
     5161 cgtcgtttcg acgcggacgc gaagcaatcg aagttagcag cagtgctcga gggattcagg
     5221 gcgtggtcgg cggccacgaa tgctgattac cgtgttgatg caatcaccgg ctcggcgctc
     5281 gccgacatgg atcgcgcgat tgagcgtctc gaatccgcgc agggcgatgc ggcgctggct
     5341 gcgcaggcgc gattgatcaa gtcgaacatg ttttactact tccacgtcga ctggcgggag
     5401 gcgcgcgcgg aggcgcagcg cgcggccgcg gcgttccgct cgatgccggc gccagatcgg
     5461 ctgggtgagg cgcgcgcgca atttgccgaa gcccgggcgc tggtgaagat ctccgccaac
     5521 agcaaatccg tggaccccac tgccggcgag gccgcgaaac tcgcgcgcga gtcgctaact
     5581 actctcggat ccgccacatc cgtgttcggt cctgtcgaac gcgcgggcgc catcggtgcg
     5641 ctgggtcata tcgacttcgg cacggcgatg ctcgacgccg ccgaaaagcg ttttgaagaa
     5701 tcgcgggcga tgtacgaggc agcgggttac agcgcgggtg aactcgagat gcgttgcaac
     5761 ctcgccgcgg tgccgctcga gcggggtcag tggcgcgttg cggcggaggc attcgtagag
     5821 ttgcttccgg aactggacaa gatttccaat cccgagcttc gcgcaatgat gtacctgggc
     5881 gcagcgcgcg ggcagtcgtt ctccgggcgc acggaccagg ccgcggagaa tcagctcaag
     5941 gcgctcgcgc ttgcacgcga gtacaagctg cgagcacagg aggccagtgc gctgcacggg
     6001 ctggcgcacg tctatcaaaa ccgtggcgac acgctgcagg cgagggcgtt cttcgccgaa
     6061 gtgctcagga tcgcgcgcga cggcgacgac gtcatggagt acgtctgggc gctggcgtca
     6121 gcgggcaccg tggcgcgtga cgacggggac tttgcgcggg ccatcgagat gcacaaggaa
     6181 gcggtgagcc gcgcctcgaa tccaatcggt ctcgtgcgca cgctgcgtga gcttggccag
     6241 gattactact tcgccaggga ctacccggca gctattgttg aatttcgaaa atcgctggcg
     6301 gtggacctgc acgacccgac ccaccacgcc tacagcgacg tgaagcgcaa tcttgcgcag
     6361 gctctcatcg acaacggcga gcgcacgcct gccacattca aggaggcggc gaaactgctc
     6421 gacgagactt tgcaaagctc gctgcgggtc agcgacaagc agggtgtgct cggcgcgcac
     6481 cgcgtcaggg cgcagctctt cgcggaacag ggcaaacctg cagaggc
//
LOCUS       DASQJS010000251         9273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2971925, whole genome shotgun sequence.
ACCESSION   DASQJS010000251 DASQJS010000000
VERSION     DASQJS010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2971925"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1869)
                     /gene="ggt"
                     /locus_tag="VEW08_10135"
     CDS             complement(163..1869)
                     /gene="ggt"
                     /locus_tag="VEW08_10135"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYJ41141.1"
                     /translation="MKLSASRVLRTFACITLVFVSSLQAATPETRIPAAGPGHAAIAS
                     AYPLASDAGKEILAKGGNAFDAAVAVAAALAVVEPSSSGLGGGGFFLLRRAKDGLETM
                     IDLREMAPGAASRDMYLDKDGNVIAGLSRDSALAAGIPGEPAGLAHLAKKYGKLPLTV
                     SMAPAIKLAREGFPLYTRLRGGMEFKKNAFLKTPDATRVFLVNGQVPELGSIIKQPEL
                     AASLELLATQGADGFYKGAFAKKLVAGVRELGGNWTEADLANYKVVERPPVVGHYRGA
                     RIVSGSPPTSGGIALIDALNILDGFDLAKVDKVTRTHLIVEAMRRVHRDRAVYLGDPD
                     FVDVPVARLINEYYAAGQRASIRMDRATPSAMLPGVDAPSPGPSTTHFSVIDAKGNMV
                     AATITLNFFFGSGLMAPGTGILLNNQMDDFSVKPGVPNGFQLIGGDANAIAPKKRPLS
                     SSTPTFVEGPNGLMIIGSPGGSYIPGMVILGTLNFIEGKTAQEIVAAPRFHHQFSPDV
                     LQFETDALNAEERKELEGRGHTLREGTRKWGNVQVVTWDYKTGKVEAASDPRGAGVGL
                     VY"
     gene            complement(1875..2357)
                     /gene="coaD"
                     /locus_tag="VEW08_10140"
     CDS             complement(1875..2357)
                     /gene="coaD"
                     /locus_tag="VEW08_10140"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HYJ41142.1"
                     /translation="MKRSAVYPGTFDPITNGHQDLVRRAASVFERVIVAIASNPNKAP
                     MFPLEQRVELARAVLGDIPGVEVMGYKGLTVDLARELGVQVVVRGLRAVSDFEFEFQL
                     ANMSRHLHRELETVFLTPQEQFTFISSTLVREIAILGGDVKEFVHPLVAAELKKMKRV
                     "
     gene            complement(2370..3191)
                     /gene="rsmD"
                     /locus_tag="VEW08_10145"
     CDS             complement(2370..3191)
                     /gene="rsmD"
                     /locus_tag="VEW08_10145"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HYJ41143.1"
                     /translation="MTARAVAAISLFMSSLLGILFAENQAGRIPSPCRTMRRTCRIGA
                     VGYAWAPKGGKQRARGFRALAKVENSGKSRKPARGHDAGGGARASASHSRILRIIAGR
                     HRGRRLRFPAGVEIRPTPDRVRETLFNWLQPRIDQSRVLDLFAGSGALGLEALSRGAA
                     QVTFVEKDRRAAAAIEAITLEWQESNARVVCESAIAWLASPASETPFDIVFLDPPYDS
                     DLLAAAAAALGGRIAPDARIYLERRAREPLPALPASWKELRAGRAGEVGYHLFSV"
     gene            3154..5403
                     /locus_tag="VEW08_10150"
     CDS             3154..5403
                     /locus_tag="VEW08_10150"
                     /inference="COORDINATES: protein motif:HMM:NF023683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41144.1"
                     /translation="MKRLIAATALAVIVTAGCSSSVDNPPDVGPTVPTTGNLAPPITT
                     TLSPNRVLFSPNQGILPYPHDAYFTPTPGVATDGTLNLPSSAFFPATLRLPSGVTEPV
                     VNALDGFSTQAPIKLRFSTQINLATSAAGIRLIEVHVDPATKATVGFPTTSSPVRRVL
                     VQGVDYIVETAPNLDTNGTLVQIVPLRPLLANNDAPGATRPRDIGYLVLVTNALRATN
                     GAAIDADADYATIKTAALANTCATITDARLNALCNLARAHFGAAGVAGVSPATIVVSS
                     SFTTQSVDTVLRVISATVAASPPPATAVLAAPVIPTGVVVAGGPNLADLHFATITLPY
                     YLSRPIPPPAGTGTEPINTWWRALNPPPAAAGLADPKGEFNLTRFNPIPRATTGLTVP
                     MLVGVPRTPKPGAGWPVVIYQHGITEDRGTLALIADVAAQAGFVIVGIDLPLHGIMPN
                     DPLYALSPTNPMGPAFPGITTPFQVPEPTFNVDYVNNTTTAPGPDGIPDSSGQHFINL
                     ASVLTARDNLRQGAANLMALTRAVPYLDVDGDGTVDIDVDRIHFFGWSLGGIIGSAYM
                     GTPIAVANVKSVSLFAAGCGIMETLRQSPGYSPVLNNGLAAAGFPPGTSIYWEFVHAA
                     QAAAESGDGCNYAANWVAKPTLMQMIQGTAGNATRPTDQAVPNSSTLRLANLLGLPTI
                     TATLGPVAGGARGLTRFTEGGHGTIALPSPAPQTLPSYLEARNQLRWFLETGGTEIRI
                     ANPAILAPQ"
     gene            5484..6434
                     /gene="ftsY"
                     /locus_tag="VEW08_10155"
     CDS             5484..6434
                     /gene="ftsY"
                     /locus_tag="VEW08_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HYJ41145.1"
                     /translation="MAESPSPKQGFIARMRAKINRPGSWLSYDLANLVPGREIDAEVL
                     DELETRLLGADVGVDATEHILEELRKKVARKELGDVDALIAALTDAITEILLPSQKPL
                     ELNAKPFVLLVVGVNGSGKTTTIGKLARRFADARKHVVLAAGDTFRAAAIEQLGVWAD
                     RAGADIIQQQAGADPGAVVFDAVQAARSRRADVVIIDTAGRLHSQSHLMDELKKVKRV
                     IQRVEPSAPHEVLLVLDANQGQNALTQATQFHEAVGVTGLVLTKLDGTAKGGIVIAIA
                     RKLGLPIRFIGVGEQAEDFGEFDARAFAGALVSGNSVAAS"
     gene            6444..7175
                     /gene="ftsE"
                     /locus_tag="VEW08_10160"
     CDS             6444..7175
                     /gene="ftsE"
                     /locus_tag="VEW08_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HYJ41146.1"
                     /translation="MITFENVAKRYPNGREALAGVTFTIQPGEFVFLTGRSGAGKSSV
                     LKLIALLERPSRGTVTVAGQNTGKLKAGKIPAFRRAIGVVFQDHKLLVDRPIYDNVAL
                     PLVVAGTPLREVDRRVRAALDQVGLLGRERSSPLELSVGEQQRVGIARAVISKPQLLI
                     ADEPTGNLDPALSVEVMRVFKRFQEVGVTVIIASHDPHLVEQFGQREIVLDAGRIATD
                     TFRPGYAARMTAQAPQLSDLPDIHT"
     gene            7186..8115
                     /gene="ftsX"
                     /locus_tag="VEW08_10165"
     CDS             7186..8115
                     /gene="ftsX"
                     /locus_tag="VEW08_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HYJ41147.1"
                     /translation="MSGAVSSYGTRHLQALFGSLGRLARAPLATMLTLMVIGVALALP
                     AGLALMVDNLRTATGDLSNAVDFTVHFKLGTPIERVEQIAKGARDRPGIASITVRNAS
                     QALEEFKQASGFGDALSALEDNPLPHHLVVRPGKNASGPTEVESLRRYFAAFPEVDIV
                     QLDLDWVRRLHSLLDVLRRVMWLVVAVLGLGVLAVIGNTIRLEIQQRRPEIEVTKLVG
                     GSNAFVRRPFLYTGIFYGLGGALLAALIVFGGLAWLDQAVGELSAQYGSRFHLTGLGG
                     QGLGILVGVGAVLGWLGALISTGRHLRAIEPRA"
     gene            8258..9148
                     /gene="rpoH"
                     /locus_tag="VEW08_10170"
     CDS             8258..9148
                     /gene="rpoH"
                     /locus_tag="VEW08_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007939338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HYJ41148.1"
                     /translation="MTATALAATALVARKNDFVLSGPVGSFDAYMDKVSRIPVLSRED
                     EAELANRFRNDGDLEAARQLVLSHLRFVVHIARGYSGYGLPLGDVVQEGNVGLMKAVK
                     RFDPTVGVRLVSFAVHWIRAEIHEYVLRNWRLVKVATTKAQRKLFFNLRKMKKNLAWL
                     SHDETLAVARDLKVTPAEVTEMEKRLAARDLSFDPVPDSGSEDGDETYSPAAYLPAPD
                     ADPAMQIENAEWEDTTGDRLAQAMKTLDPRAQDIVVSRWTGEGKVTLHDLAEKYGVSA
                     ERVRQIEANAIKKLRGLMTA"
BASE COUNT         1663 a         3087 c         2900 g         1623 t
ORIGIN      
        1 gtacctgtgg cgggcggtcg atttgcatcg cgagggcaaa ctacccaagc ccgcgacgcc
       61 cacgcgcatc aagatagtga agaagaaaaa ggccgtgaaa aaggttgccg cgaagaaatc
      121 gaaacgtgtc gcgcgcaagc gcagaccaaa gtaacgcggt agtcagtaaa ccagcccgac
      181 acccgcgccg cgcggatcgg acgcggcttc gaccttgccg gtcttgtagt cccacgtgac
      241 cacctgcacg ttgccccact tgcgagtacc ttcgcgcagc gtgtgaccac gcccttcgag
      301 ttccttgcgt tcttcggcgt tgagcgcatc ggtttcgaac tgcagcacgt ccggcgagaa
      361 ctggtgatgg aagcgcggcg cggcgacgat ttcctgcgcg gtcttgcctt caatgaaatt
      421 gagcgtgccg agaatgacca tacccgggat gtagctgccg cccggcgatc cgatgatcat
      481 cagcccgttg gggccttcga cgaacgtggg cgtggacgaa gacagcggcc gcttcttcgg
      541 agcaatagcg ttggcatctc cgccgatgag ctggaagcca ttgggcacac cgggcttcac
      601 cgagaaatcg tccatctggt tgttcagcag gatgccggtg ccgggcgcca tgagcccgga
      661 gccgaagaag aaattgagcg tgatggtggc ggccaccatg tttcccttcg cgtcgatcac
      721 cgagaagtgc gtggtcgagg ggcccggtga cggcgcatcg acacccggca acatggcgct
      781 cggcgtggcg cgatccatgc ggatcgacgc gcgttgcccg gcggcgtagt actcattgat
      841 gagccgcgcc accggcacgt cgacgaaatc cggatctccg agatacacgg cgcgatcgcg
      901 atgcacgcga cgcatggctt cgacgatgag gtgcgtacgc gtgaccttgt cgacctttgc
      961 gaggtcgaag ccgtcgagga tgttcaaggc atctatcagc gcgatgccac ccgaagtcgg
     1021 cggtgaaccc gagacgatgc gcgcgccgcg gtagtggccg accacgggcg gtcgctcgac
     1081 caccttgtag ttagctaggt cggcctcggt ccagttgcca ccgagctccc ggactccggc
     1141 cacgagcttt ttcgcgaacg cgcccttgta gaaaccgtcc gcgccctggg tggccagcaa
     1201 ttcgaggctg gcggcgagtt cgggctgttt gatgatggag ccgagttcgg gcacctggcc
     1261 attcacgaga aacacgcgtg tggcgtccgg cgttttgagg aacgcgttct tcttgaattc
     1321 catgccgccg cgcaggcgcg tgtacagcgg aaagccttca cgcgcgagct tgatggcagg
     1381 cgccatgctc accgtcagtg gcaacttgcc gtatttcttc gccaggtggg caagacccgc
     1441 cggctcgccg ggaattccgg ccgccagcgc cgaatcgcgt gaaaggccgg cgatgacatt
     1501 gccatcttta tcgagataca tgtcgcgcga ggcggcgccc ggtgccatct cgcgcaggtc
     1561 gatcatggtt tcgaggccgt cctttgcccg tcgcagcagg aagaacccgc caccgccaag
     1621 acccgaactc gagggttcga cgaccgccaa cgccgcggcc acggccacgg ccgcatcgaa
     1681 agcattgccg cccttcgcga ggatttcctt gccggcgtcg ctcgccagtg gataggcgct
     1741 ggcgatggcg gcgtgacccg gcccggcagc gggaatcctg gtttcaggtg tggccgcctg
     1801 cagcgacgac acgaaaacga gggtgatgca ggcgaatgtg cgtaaaacgc gcgatgcgga
     1861 caacttcatg cgtttcagac ccgcttcatc ttcttcagtt cggccgcgac cagcggatgc
     1921 acgaactcct tcacgtcgcc accgagaatt gcgatctcgc gcaccagggt ggaggaaatg
     1981 aaggtgaatt gttcctgcgg ggtgagaaac acggtttcga gctcgcggtg caggtgtcgg
     2041 ctcatgttcg cgagctgaaa ctcgaactcg aaatccgaca cggcgcgcaa tccgcgcacc
     2101 acgacctgca caccaagctc gcgggcgaga tccacggtca gccccttgta tcccatgacc
     2161 tcgactcccg gaatgtcgcc gagtacggcg cgcgccagtt ccacgcgctg ttcgagcgga
     2221 aacatcggcg ccttgttagg gttcgaggcg atcgcgacga tgacccgttc gaacacgctc
     2281 gcggcccggc gcaccaggtc ctggtgtccg ttggtgatgg gatcgaaggt ccccggatat
     2341 acggcgctgc gtttcatgcg gccctggatt caaacagaaa atagatgata cccgacctcg
     2401 ccggccctac ccgcgcgaag ctctttccag ctcgcgggta acgccggcag cggctcccgg
     2461 gcccgccttt cgagatagat gcgtgcgtcg ggagcgatcc ggccgccaag tgcagccgcc
     2521 gccgccgcga gcagatccga atcgtagggg ggatcgagaa acacgatgtc gaacggagtt
     2581 tcgctggccg gcgacgccag ccacgcgatg gcgctctcac aaaccacgcg cgcattcgac
     2641 tcctgccatt cgagtgtaat tgcttctatc gccgccgcgg cacgccgatc tttctcgacg
     2701 aaagtgacct gcgcagcgcc gcgcgacagc gcttcgagac cgagcgcacc gctgccggcg
     2761 aacaagtcga gcacgcgcga ctgatcgatg cgcggctgca gccagttgaa cagggtttcc
     2821 cgcacgcggt ccggagtcgg gcggatttca acgccggcgg gaaagcgaag gcgacggcca
     2881 cgatgccggc ccgcgatgat gcgcaggatt cgcgagtggg acgcgcttgc tcgtgcacca
     2941 ccgcctgcgt catggccgcg cgcaggcttt cgactttttc cgctgttttc tactttcgcc
     3001 aatgctcgaa acccacgcgc ccgctgcttg ccaccttttg gggcccaagc gtaacctacc
     3061 gccccaatac gacaagttcg gcgcatcgtt ctgcacggcg agggaatgcg accggcctga
     3121 ttctccgcaa acagaattcc gaggagcgaa ctcatgaaga ggcttattgc agccactgcc
     3181 ctggctgtca tcgtcacggc cggttgttcg agttcggtgg acaacccacc ggatgtaggg
     3241 ccaaccgttc cgacgactgg caacctcgct ccgccgatca cgaccacgct gtcgcccaac
     3301 cgcgtgttgt tctcgccgaa ccagggcatc ctgccgtacc cgcacgacgc ctacttcacg
     3361 cccaccccgg gtgttgccac cgacggcacg ctgaacctgc cgtccagtgc cttcttcccg
     3421 gcgacgctgc gcctgcccag cggcgtaacc gagccggtgg tcaacgcgct cgatggattc
     3481 tccacgcagg cgccgatcaa gctgcgtttc tccacacaga tcaacctcgc cacctcggct
     3541 gccggcatcc ggctcatcga agtgcacgtc gatccggcca ccaaggcgac ggtgggattc
     3601 cccaccacct cgagcccggt tcgtcgagta ctggtccagg gtgtggacta catcgtcgag
     3661 acggcgccta acctcgatac caacggcacg ctcgtgcaga tcgtgccgct gcgcccgctg
     3721 cttgccaaca acgacgcgcc cggcgcgact cgcccacgcg atatcggcta cctcgtgctg
     3781 gtcaccaacg cgctgcgcgc cacgaacggc gccgccatcg atgcggatgc tgactacgcc
     3841 accatcaaga cggcggcgtt ggccaacact tgcgccacga tcaccgatgc gcgcctgaac
     3901 gcgctgtgca acctggctcg cgcgcacttc ggcgctgccg gcgtcgcggg cgtgagcccg
     3961 gcgaccatcg tcgtttcctc cagcttcacc actcaatcag tggataccgt gttgcgcgtg
     4021 atctcggcca cggtggccgc gagcccgcct cccgccaccg ccgtgttggc ggcgccagtg
     4081 attcccaccg gcgtggttgt ggccggcggt ccgaacctcg ctgatcttca cttcgccacc
     4141 atcacgctgc cttactactt gagtcggcca ataccgccgc ccgcaggcac gggtaccgag
     4201 ccgatcaaca cctggtggcg cgcgttgaat ccgccgccag ccgccgcagg tctcgccgac
     4261 cccaagggtg aattcaacct gacccgcttc aatccgattc ccagggccac gactgggctc
     4321 actgtaccga tgctggtggg tgtgccaaga acccccaaac caggcgcggg atggccggtc
     4381 gtcatctatc agcacggcat caccgaagat cgcggcacgc tcgcgctcat cgcggatgtc
     4441 gccgcgcagg ccggattcgt catcgtcggc atcgatctgc ccttgcacgg catcatgccg
     4501 aatgatccgt tgtatgcgct gtcgcccacg aatcccatgg gccccgcatt tcccggaatt
     4561 acgacgccat ttcaggttcc cgaacctacg ttcaacgtgg actacgtgaa caacaccacc
     4621 acggccccgg gacccgacgg catccccgat tcgtccggcc agcacttcat caatctcgcg
     4681 agtgtcctca ccgcgcgtga caacctgcgg cagggtgcgg cgaatctcat ggcgctaaca
     4741 cgcgccgtgc cgtacctgga tgtcgacggc gatggcacgg tggacatcga tgtggacagg
     4801 atccacttct tcggctggtc gctcggcggc atcatcggca gcgcgtacat ggggaccccg
     4861 atcgctgtgg cgaacgtcaa gtcggtatcg ttgttcgcgg cgggctgcgg gatcatggaa
     4921 acgctgcgcc agtcgcccgg ttattcaccc gtcctcaaca acggcctcgc ggccgcaggt
     4981 tttccaccag ggacttccat ctactgggag ttcgtgcatg ccgcgcaagc cgcggcggaa
     5041 tccggggacg gctgcaacta cgcggcgaac tgggtcgcga agccgactct catgcaaatg
     5101 atccagggga cggctggcaa cgccacgcgt ccgacagatc aggcggtacc caactcctcg
     5161 accctgcgcc tcgcgaatct gctgggcttg ccgacgatca ccgccactct gggcccagtg
     5221 gcaggcggcg cgcgcggcct cacccgcttc accgagggtg ggcacggcac gatcgcgctt
     5281 ccgagcccgg cgccgcagac gctaccgtcc tacctcgagg cccggaacca gcttcgttgg
     5341 ttcctggaaa ccgggggcac ggagatcagg atcgccaatc cggcgatact cgcgccacag
     5401 taagataaag gcgcatctga cagacagagc ggcccggaag ggccgctctt tttttcacca
     5461 cacgcaaagg atagaaaagg cagatggcag agagcccttc gcccaagcaa ggattcatcg
     5521 cgcgcatgcg cgcgaagatc aaccgtccgg gttcgtggct ctcgtatgac ctcgcgaatc
     5581 tcgtccccgg gcgcgagatc gacgccgagg tgctcgatga gctcgaaacg cgcctgctcg
     5641 gcgccgacgt cggcgtcgat gcaaccgagc acatcctcga agagctgcgc aagaaggtgg
     5701 cgcgcaagga gcttggcgat gtcgatgcgc tcatcgcagc gctcacagat gcaatcaccg
     5761 aaatcctgct gccttcgcag aagcctctcg agctcaacgc caagcccttc gtgctgctcg
     5821 tggtcggtgt gaacggctcc ggcaagacca ccaccatcgg caaactcgcg cgccgtttcg
     5881 ccgacgcgcg caaacacgtg gtcctcgcgg cgggcgatac gtttcgggcc gcggccatcg
     5941 agcagctcgg tgtctgggcg gaccgcgccg gcgcggacat catccagcag caggcgggcg
     6001 cggatccggg cgccgtggta ttcgacgccg tgcaggcggc gcgctcgcgc cgcgcggacg
     6061 tggtcatcat cgacaccgcc ggccggctgc acagccagtc gcacctcatg gatgagctga
     6121 aaaaagtgaa gcgcgtgatc cagcgtgtcg aaccttccgc accgcacgag gtcttgttag
     6181 tgctcgacgc caaccaggga cagaatgcac tcacgcaggc cacgcagttt cacgaagccg
     6241 tgggcgtcac gggcctcgtg ctcaccaagc tcgacggcac cgccaagggc ggcatcgtga
     6301 tcgccatcgc gcgcaagctg ggcctgccca tccgcttcat cggcgtgggc gagcaggccg
     6361 aggatttcgg tgaattcgac gcacgcgcct tcgctggtgc gctggtttcc ggcaacagcg
     6421 tggccgcgag ctaagatccg cgcatgatca ccttcgaaaa cgtcgcgaag cgctatccga
     6481 atggccgcga ggcactcgcg ggcgtgacgt tcaccatcca gcccggagaa ttcgtgttcc
     6541 tcaccggccg ctccggggcc ggcaagagca gcgtcctgaa actcattgcg ctgctcgaac
     6601 gcccgagccg cggcaccgtc accgtggccg gacagaacac gggcaagctc aaggccggca
     6661 agattccggc attccgccgc gccatcggcg tggtgttcca ggatcacaag ctgctggtgg
     6721 accggcccat ctacgacaac gtggcgctgc cgctggtggt cgccggaacg ccgctcaggg
     6781 aagtcgaccg gcgcgtgcgc gccgcgctcg accaggtcgg gttgttaggc cgggaacgtt
     6841 cttcaccgct cgaactctcc gtgggcgagc agcagcgtgt cggcatcgcg cgcgctgtca
     6901 tcagcaagcc gcagctgctg atcgccgacg aacccaccgg aaatctcgac cccgcactca
     6961 gcgtcgaagt catgcgtgtg ttcaagcgct tccaggaagt cggcgtcacc gtgatcattg
     7021 cctcgcacga cccgcacctg gtggagcagt tcggccagcg ggagatcgtg ctcgatgccg
     7081 gtcgcatcgc cactgacacc tttcgacccg ggtacgcggc acgcatgacc gcgcaggcgc
     7141 cgcagctttc cgatctgccg gacattcata catgagcagt ggtacgtgag cggggcggtt
     7201 tcgtcatacg gcactcggca tctgcaggcg ctgttcggct cgctcggccg gctggcccgt
     7261 gcaccgctcg ccaccatgct cacgctcatg gtcatcggcg tggcgctggc cctgccggcg
     7321 ggactcgcgc tcatggtcga caacctgcgc accgctaccg gcgacctcag caatgccgtc
     7381 gacttcaccg ttcatttcaa gctcggcacg cccatcgagc gcgtggagca gatcgccaag
     7441 ggcgcgcgcg atcgcccggg cattgcctcc atcaccgtca ggaatgcctc gcaggcgctg
     7501 gaggagttca agcaggcctc gggcttcggc gatgcgctgt cggccctcga ggacaatccc
     7561 ctgccgcacc acctggtggt tcgccccggg aaaaacgcca gcggccccac cgaggtggaa
     7621 tccctgcgcc gctatttcgc cgcgtttccc gaggtcgata tcgtgcagct cgatctcgac
     7681 tgggtacgtc gcctgcattc gctgctcgat gtcctccggc gcgtcatgtg gctggtggtg
     7741 gccgtgctcg ggctcggggt actggccgtg atcggtaaca ccatccggct cgagatccag
     7801 cagcggcggc cggaaatcga ggtcacgaag ctggtcggcg gctcgaacgc tttcgttcga
     7861 aggccgttcc tgtacaccgg catcttctac ggccttggcg gggcgctgct ggcggcgctc
     7921 atcgtgttcg gcggccttgc ctggctggac caggcggtcg gcgagttgtc cgcgcagtac
     7981 ggcagccgtt tccacctgac gggcctcggc gggcaggggt tggggatact cgtgggagtc
     8041 ggggcggtac tcggctggct gggcgcgctc atctccaccg gccggcacct ccgggcgatc
     8101 gaacctcgcg cctaggcctc cgcttataaa acaataactt atcggtctga tgcctccttc
     8161 ggggtatgga actgaccggg cttttagcac tctaattgcc cgactgctaa tatctcgacc
     8221 ctcgagataa gcagatcgcc tcaggaggtt cgtccagatg actgccaccg cccttgctgc
     8281 cactgcgctg gtcgctcgca agaacgattt cgtcctgtcc ggtcccgtcg gaagcttcga
     8341 cgcgtacatg gacaaggtaa gccgcattcc cgtgctgtcg cgcgaggatg aggctgaact
     8401 cgccaatcgc ttccgcaatg acggcgatct cgaggccgcc cgccagctcg tgttgtcgca
     8461 cctgcgtttc gtggtgcaca tcgcgcgcgg ctattcgggt tatggcctgc cgctcggcga
     8521 cgtggtccag gaaggcaacg tgggactcat gaaggccgtg aagcgcttcg accccacggt
     8581 tggcgtgcgc ctggtttcgt tcgcggttca ctggatccgc gcggaaatcc acgaatacgt
     8641 gctgcgcaac tggcggctcg tcaaggtcgc caccaccaag gcgcagcgca agctgttctt
     8701 caatctgcgc aagatgaaga agaacctggc ctggttgtcg cacgacgaaa cgctcgcagt
     8761 cgcgcgtgat ctcaaggtaa cgcccgccga ggtcaccgag atggaaaagc gcctggcggc
     8821 gcgcgacctc tcgttcgacc cggttcccga ctcgggcagt gaggacggtg acgagaccta
     8881 ctcgcccgcc gcctatctac cggcgccgga tgccgatccg gccatgcaga tcgagaatgc
     8941 cgagtgggaa gacaccacgg gcgatcgcct ggcgcaggcc atgaagacgc tggatccgcg
     9001 cgcgcaggac atcgtggtca gccgctggac cggtgaaggc aaggtcacgt tgcacgacct
     9061 cgcggagaaa tacggcgtgt cggcggaacg cgtgcgccag atcgaggcga atgcgatcaa
     9121 gaaattgcga ggcctgatga cggcctgaag cgcccgttca gactcaactt tgaagccccg
     9181 gccacaaccg gggctttttc ttttccgatg ctaagttttt gtgttgtccg ggcccgcggg
     9241 tccgtggatt aatggcaggc aatgacgaaa tca
//
LOCUS       DASQJS010000252        14940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4224675, whole genome shotgun sequence.
ACCESSION   DASQJS010000252 DASQJS010000000
VERSION     DASQJS010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14940
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4224675"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VEW08_10175"
     CDS             <1..963
                     /locus_tag="VEW08_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ41149.1"
                     /translation="AGDKGFPSVNDPCASTRANGTTRVVPADVAAFCQQTWGINAATY
                     TQTNSQIEALFYGNPNLSEETSDTLTFGFALTPESIPDLHLSVDYYKIKVRDFVNTLE
                     GGPTGVVLACFASLDIDSDACFSRDLNEALVFRDAVGDLKARVPIANLSALETDGIDV
                     SVSYDVPLGFTSERFDDSLQVNLLVTWLGSYDLDHVDYAGTIGGYNISGAFPEYKANL
                     RLGYRIGPVNLSYNLQFLDAMDNQGNIPAFEDPAGYQGVTSTIFHDLSARWDIKDSLD
                     LTVGVRNLTDEDPKFFDVPVDQNTDPATFDMLGRFYFGSLRVKF"
     gene            1111..3846
                     /locus_tag="VEW08_10180"
     CDS             1111..3846
                     /locus_tag="VEW08_10180"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYJ41150.1"
                     /translation="MSRSVRDAWVQIGPYLDVVLELDAEKRDAWLAELHTRMPQVAES
                     VRKHLAELAELNARNFMNVAVPARFFESSLAGKRFGAYTLERAIGQGGMGTVWLARRS
                     DGQFEGRAAVKLLNTELIGIPSELRLTREISVLAKLQHPNIAHLIDAGVGADNRPYLV
                     LEYVVGLPIDQYCETRKLRIRHRIRLFLDVLRAVAHAHSHLVVHRDLKPSNILVTEDG
                     TVKLLDFGIAALLSTPGQSIAHTQQLAPGLTPGYAAPEQLSGEPITIATDVHALGTLL
                     FVLLSGQHPSLCHGKNRAEWMRAVLEREAPRMSDYAASVGDRRLLRGDLDHIVAKALR
                     RVADERYSTIDLFAQDLRRYFAFEPVAARPRTYAYLARMFARRHRAAVAAAVIVAVII
                     AAAMGINTRLMLEARYQRDQARFQSDRAEASIDFLTQLMRSNAEAGAGTGPQTFHERL
                     AMGVEILKRQYHEDPKFAGRMLVDLADYYRNDGETARANELYAQAYELGSKNDDKELM
                     VSAQCSRADGDAYAGIHEGTEARVREAQQLLAQIPKSDVALHIGCLLAQVTFKEQTGD
                     LAAAESILERAMKTRAEVDGTNETVAYATLLTERGAMYYLRSQPREALVATQAAADIY
                     EGKGRGRMAAGLSARFAISTILTDMGELRRALAEREIIRQRLHENGESKAEHVAYPVS
                     HGLLLLRMGRVSAAGEMLNGVVERARQAGNPHWLAWALLASGQLSLQQGHLDAAEAVL
                     IEASSLLDSGASDVNTQIFAATYLAELALARHDIELAHRYRDEFLQLADYRGKQTNHA
                     LPRVLIVAARIALAEGAAADAERFARDGLRLAQRKSRGPDTSADVGEALLRISQARLL
                     ANPGADVGSMLGRADRCLTAALDANHPLTIEVRKLGKRPGAIGPRADIGSAAGVP"
     gene            3881..4450
                     /locus_tag="VEW08_10185"
     CDS             3881..4450
                     /locus_tag="VEW08_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41151.1"
                     /translation="MKARISPLMFIATLLLVAATASADEFDDTVSIFKNAGESGTYFA
                     KSYGYAVFPTVGKGGLGVGAAHGSGRVFAQGKHVGDVKMNQISVGLQAGGQAFSQIVF
                     FQDKRSFDEFTSGSYEFDATMQAVAITASATATAGTAGAAAGAAGGKKDAATTGNYHK
                     GLAVFTVAKGGLMYQAAVGGQKFKYEAKK"
     gene            complement(4535..4816)
                     /locus_tag="VEW08_10190"
     CDS             complement(4535..4816)
                     /locus_tag="VEW08_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41152.1"
                     /translation="MKRILPIAVLWAVAVVPALGCDRPTAPTSIPDGASASKEDMLAA
                     KKAVDAFKGSMEEYLACEKSGTKIESAQKELVKVADRFNLQVRAFKAKG"
     gene            5029..5544
                     /locus_tag="VEW08_10195"
     CDS             5029..5544
                     /locus_tag="VEW08_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HYJ41153.1"
                     /translation="MQHDKESREKAGASGDGIRKVLTFSLGGEVYGVDILRVKEIRGW
                     SPVTRIPQTPASILGVLNLRGLIVPIVDLRVRFALKTAEFTPLTVIIVLSLRNESGQR
                     ECGIVVDNVNDVVDIATDSIKPAPELGGGQTGEYIEGITSHEEQMLILLNAESVASPD
                     LSATVLAEQAA"
     gene            5592..8339
                     /locus_tag="VEW08_10200"
     CDS             5592..8339
                     /locus_tag="VEW08_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYJ41154.1"
                     /translation="MRLNVFKNIRLSLQLYALVAFTLVIATGLIFYAMEQVRSTQGTL
                     KHTIDNRMVSGQSIQGVADALSLSIEASLDVIEKKQSALDAHDQIKKAIDKARDDWDN
                     YFLGDMIPEEQELADETTPMLDGAYGKIDKLLKKLESNELADLPAWRNETLRPALAEG
                     SSNLKKLIAMQLTAANLDLARANTNYQQAVRNSITLMSGGAFLAIVLAWIIIRSAMRK
                     LGADPGRAAQVARRVASGDLEFEMLPGRHDAISLMGALRQMKDSLLHSKLDYQGQINA
                     IAKVQGVIECSLDGDVANANDIFLNLLGYTPDDVKGKNYSLFVDSQDSAANQGLWKAL
                     KGGESQQGEFRVMARDKRELWFQGVFNPIVDASGKPFKVVAYLSDITRQRQEALLNAA
                     FRGALDRLDANVMCADNDMKIIFVNPAATRMLARAQESFRADLPGFDAGKIMGANLES
                     MTREPAVLRGLIERLTDIVNRQEVIGGRTMKTIMSPMKDDTGRRLGTVLEWFDRTQEV
                     ATESELQDVITAVTAGNLDNRISLEGKRDFFLTLSRGINELVEAIATVVVEVRGVVAA
                     ANTGDLTRRIETQGKAGLLVQIGSGINDLTSSLSELVSQVKKASAEVSRGADEISQGN
                     TNLSQRTEEQASSLEETASSMEEMTSTVKQNADNAAHASKLGYAARDQAEKGGAVVSK
                     AVAAMVGINDASKKIADIISVIDGIAFQTNLLALNAAVEAARAGEQGRGFAVVATEVR
                     NLAGRSAGAAKEIKALIQDTVSKVNEGSNLVTQSGTTLGEIVSAVKKVTDIVAEIAAA
                     SNEQAAGIDQVNKAVMQLDELTQQNAALVEEASAASQAMAEQARDLNSSMSRYQVNAT
                     AAEAAAPESTVPAAPTLAADRRKATRPWTEKPAAAAKAPAARASARVANGADETEWKE
                     F"
     gene            8427..10685
                     /locus_tag="VEW08_10205"
     CDS             8427..10685
                     /locus_tag="VEW08_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYJ41155.1"
                     /translation="MQFPRRLQCVVLLAGALQALTPVVSVGAEAPHSAGRPRVGLVLA
                     GGGAKGGAHVGVLKVLEELHVPIDCIAGTSMGALIGGGYAAGIPADELEKFLVNIDWK
                     KVVGSQGRRDLEPIEQKRSGPTYSNDFELGITPNGVVTPGGLINTSNIEDLLRVYVAR
                     ARLETDFDKLPIPYRAVATDMVSSKMVVLEQGDLATAMRASMAIPGGFAPVIMDDMIL
                     SDGGLVRNIPIDIARNLCAEQVIVVNLVEPEADPVKLQSATQLLGRSMDVMIIANEEL
                     QLASLRPGDVRIDVIMGDIGTADFERVPDTVPLGVTAAHKMSDELRKFSVPEAQYVAW
                     RTAVTQSQQIEGRLAGVRFEGLKRVNPEYLVRRHQVKAGDPVDIVTISEEAQRISALQ
                     DFDSVSYRLDGDRSSPTLTWLPQEKKIGPNYLRVDLGVYASREGDLSFSIYGRHVRTW
                     VNSLGAQWYNELQLGGESLVTTGFYQPLDVARRFFIEPRVTFSRALEDVFVDDERVAR
                     YEFKDFIGELNLGANVGRYGQARIGYLYDERDVDVDIGTPLMPEGKPVDAGMHVLAEL
                     DSRDTAFNPTRGLAMALEYMESDESMGGDRDWERAELGFGIALPLRRDVLWLTFAAGS
                     DLGSDLPGDRAFSVGGPDSFPGFEQGELRVSEYWLANTSYLWRVKDLLSIRNHSLYVG
                     VGLTGGQLNGRLDGGEDGEHYGGSVFLTGRTFFGPLTVGVGATSTDSWSLWLSVGRPV
                     GHGTILERGVFR"
     gene            complement(10695..12368)
                     /locus_tag="VEW08_10210"
     CDS             complement(10695..12368)
                     /locus_tag="VEW08_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HYJ41156.1"
                     /translation="MSKLPAAALQALLFITGITAGLAHAQDVSAPAPASASSELQTQV
                     EAMLESGPFEIHGAHVAYPNVIYAFYTRRSFQPAWTNAHTASELRRALKDSEADGLDP
                     RDYHLAVLEQLAAGAAGSPSAQAAFEILHTDALLRIADHLMFGKVDAASFDAQWNYTR
                     SPAGIDVPQRLELAIASDDIHAEIEKLKPTHRMYRTLKHELARFREAAAGGEWVALTQ
                     ETNNGKSIDPGAHDERVPALRKRLSISGDLDAAEVTSSDLYDPTLQSAVRRFQERMGL
                     VADGKIGARTISELNVSFDERISQLRVNLDRGRVLLHDLPNEFVVVNIAAYWVYLVRG
                     QEIVWSARAQVGKTYRRTPMFRSDINYLVFNPTWTVPPGIIRNDILPDARRDPASITR
                     RGLNVLDARGNIIDPASIDWSKFKSGNIPYTLRQDPGPKNALGRVKIMFPNSYSIYLH
                     DTPSQAKFEETDRAFSSGCVRVERPLELAELLLANPATWNAGTIRKTIDAGNTQNVTL
                     PAKVPVLLAYWTAWVDPQGRVNFRRDIYGQDAQWLAGLARPYTPRKLPN"
     gene            12587..13402
                     /locus_tag="VEW08_10215"
     CDS             12587..13402
                     /locus_tag="VEW08_10215"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYJ41157.1"
                     /translation="MKTKPAKHFLLASDFDQTLSFNDSGFELSELLGVTSFERKVAGL
                     SASHLVQQGGELAYLIRHDPEFRGVRREHLIEAGKRVRLKQGLPEFVEILERGIDGNQ
                     FSFYVVSAAPREVVVSALEGIVPPEHIYGTELDYDPHSGEVRSINRVRAGYGKVAVLD
                     ELGQKLGITTDRVIYVGDGSSDIHVMLHVNNGDGFTIAVSENRQLARIAQSTVLSDNA
                     SSVLVPVLDQVLGLRPGYIRAQLESTGLVLDDWQRARTDRVLIREAAPLASAG"
     gene            13411..13632
                     /locus_tag="VEW08_10220"
     CDS             13411..13632
                     /locus_tag="VEW08_10220"
                     /inference="COORDINATES: protein motif:HMM:NF022152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HYJ41158.1"
                     /translation="MTDMLGWVATAVFVGSYFFSRPALLRAAQMVGATLWIVYGALIG
                     AIPVVVANGLVFAAAAWTLFRARPPLTQT"
     gene            13679..14557
                     /locus_tag="VEW08_10225"
     CDS             13679..14557
                     /locus_tag="VEW08_10225"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005091.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HYJ41159.1"
                     /translation="MTDITLIGPGAIGSTLAAWLSQNRDHQVTVAARTPFDSIVLETP
                     RGTLNASPRVLTDPSQARPADWVLVTTKAYDSDAAARWFEKSFGPRTRLAVIQNGVEH
                     VERFVRYVAADRIVPVMIDCPATRHSPGRVTQGGPIHIVVPAGVNGAAFVDQFAGLSL
                     DVRETPDFITAVWKKLCLNSAGAVSTALLAPAGIAHHDGVAEILRAIVRECIAVGRAR
                     GAVLGDEIAGEIVDRMRSGPRDAANSMLADRRAGRPMEIDARNGVIVRYGREHGIPTP
                     MNALMVAMLEAVDQCS"
     gene            complement(14554..>14940)
                     /locus_tag="VEW08_10230"
     CDS             complement(14554..>14940)
                     /locus_tag="VEW08_10230"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ41160.1"
                     /translation="HAEVLKIALRAKRHDRFMLVSDAMPSVGADVKEFRIQGRIITVD
                     GDRIIDDEGRLAGAHLDMASAVRNTVNQLGVSLTDALRMASTNPAGFLKLDGRMGRIA
                     PGQRADLVLVDEELSVLRSWIGGLAD"
BASE COUNT         2809 a         4684 c         4798 g         2649 t
ORIGIN      
        1 gccggagaca agggctttcc ttcggtcaac gatccgtgcg catcgacgcg cgccaatggc
       61 acgacgcgcg tcgttccggc ggatgtcgcg gcgttctgcc agcagacctg gggaatcaat
      121 gcggcgacct acacgcagac caacagccag atcgaggcgc tcttctatgg caacccgaat
      181 ctctccgagg aaacttcgga cacgctgact ttcggattcg cgctgacgcc ggaatcgatt
      241 ccggacctgc acctctcggt ggactactac aagatcaagg tgcgggactt cgtgaacacg
      301 ctcgaaggcg ggcccacggg cgttgtgctg gcgtgtttcg ccagcctcga tatcgacagc
      361 gacgcatgtt tcagccgtga cctcaatgag gcgctggtgt ttcgtgacgc cgtcggcgac
      421 ctgaaagccc gcgtgccgat cgccaatctg tcggcactcg agaccgatgg tatcgacgtc
      481 agcgtcagct acgacgtgcc gctgggattt acctcggagc gtttcgacga cagcctgcag
      541 gtgaatctgc tggtcacctg gctgggtagt tatgacctgg accacgtcga ctacgccggc
      601 accattggcg ggtacaacat cagtggcgcg tttccggaat acaaggcgaa cctgcgcctc
      661 ggttatcgca tcgggccggt gaacctcagc tacaacctcc agttcctcga cgcgatggac
      721 aaccagggca acatcccggc cttcgaggac ccggcgggct accagggagt gactagcacg
      781 atctttcacg atctgagcgc gcgctgggac atcaaggatt cgctggatct caccgtcggc
      841 gttcgaaatc tcacggacga agacccgaag tttttcgacg tcccggtcga tcagaacacc
      901 gatccggcga cgttcgacat gctcggccgc ttctacttcg gctcgcttcg cgtgaagttc
      961 taggaacagg tttcctgcaa gttgatggcg gaccgaaagt ccgccatctt tttttggcag
     1021 ttccccggct ccgtgtcggg aaccggcgcg caacgccgat ttactcaagg ggacccactt
     1081 tgaggacacg gagccgtttc catgacgatc atgagccgaa gtgtccgcga cgcgtgggta
     1141 cagatcggcc cctatctcga tgtggttctc gagctcgatg ccgaaaaacg cgacgcatgg
     1201 ctcgcggaac tgcacacccg catgccgcag gtggcggagt cggtgcgcaa gcacctggcc
     1261 gaactggccg agctgaatgc acggaatttc atgaatgtcg cggtgccggc gcgattcttc
     1321 gagagcagcc tcgccggcaa gcgatttggt gcgtacacgc tcgagcgcgc gattggacag
     1381 ggcggcatgg gcacggtctg gctggcgcgg cgcagcgacg ggcaattcga aggccgcgcc
     1441 gccgtaaagc tgttgaacac cgaactcatc ggcattccgt cagaactccg cctgacccgc
     1501 gaaatcagcg ttctcgcgaa gctgcagcat ccgaacatcg cgcatctcat cgacgccggc
     1561 gtgggcgctg acaaccggcc ctacctggtc cttgagtacg ttgtcggcct gcccatcgac
     1621 cagtactgcg agacgcggaa actccgaatt cgccatcgca tccgcctgtt cctcgacgtg
     1681 cttcgcgcag tggcgcacgc gcacagccac ctggtcgtgc accgcgacct gaaaccgtcg
     1741 aacattctcg tgaccgaaga cggcaccgtg aaactgctcg actttggcat cgccgcgctg
     1801 ctgtccacgc ccggccagtc gatcgcccac acgcagcagc tggcgcccgg tctcacgccc
     1861 ggctacgccg ccccggaaca gctgtcaggc gaaccgataa cgattgcgac ggacgtccac
     1921 gcgctgggga ctctgctgtt cgtgctgctg agtggccagc atcccagtct gtgccacggc
     1981 aagaaccgcg cggaatggat gcgcgccgtg ctcgaacgcg aagcgccgcg aatgtccgac
     2041 tacgcggcca gcgtcggcga ccggcgcttg ctgcgcggtg acctcgacca catcgtcgcg
     2101 aaggcgctgc gcagagtggc tgacgagcgc tacagcacca tcgatctctt tgcgcaggac
     2161 cttcgccgat atttcgcgtt cgagccggtg gcggcacgcc cgcggacata tgcgtacctg
     2221 gccaggatgt ttgcgcggcg ccatcgagcg gcggtggctg ccgcggtgat cgtggcggtg
     2281 atcatcgccg cggccatggg aatcaacacg cggctgatgc tcgaagcgcg ttaccagcgt
     2341 gaccaggcgc ggttccagtc ggaccgcgcc gaagcgtcga tagattttct cacccagctg
     2401 atgcgttcca atgctgaggc cggggccggc acgggaccgc agacctttca cgaacggctg
     2461 gcgatgggag tcgaaatcct caaacggcag taccacgaag atccgaagtt cgcgggccgg
     2521 atgctggtcg acctggctga ctactatcgc aacgacggcg agactgcccg tgccaacgag
     2581 ctctacgcgc aggcgtacga actcggcagc aagaacgacg acaaggagct catggtctcc
     2641 gcgcagtgca gccgcgccga tggtgacgcc tacgccggca tccacgaggg cacagaagcg
     2701 cgcgtccggg aggcacagca gctgctggcg cagatcccga agtccgatgt cgcattgcac
     2761 atcggctgcc tgctcgcgca agtgactttc aaggaacaga ctggcgacct tgccgccgcg
     2821 gagtccatcc tggagcgtgc aatgaagact cgcgcggaag tggatgggac caatgagacc
     2881 gtggcgtacg ccacgctgct caccgagcgc ggcgcgatgt actacctgcg cagccagccg
     2941 cgcgaggcgc tggtcgcgac ccaggccgcg gcggatatat atgagggcaa gggccgcggc
     3001 cgcatggcgg cggggctcag cgcgcgattc gcgatctcca cgatcctgac cgacatgggc
     3061 gaattgcgcc gtgcgctggc cgagcgcgag atcatcaggc aacgacttca cgagaacggc
     3121 gaatcgaagg cggaacatgt cgcgtatccg gtgagccatg gattgttgct gctgcgtatg
     3181 ggcagggtct ctgcggcggg agagatgctc aacggtgtcg tggaacgcgc ccggcaggcg
     3241 gggaacccgc actggcttgc atgggcactt ctcgccagcg gtcagctgtc ccttcagcaa
     3301 ggtcacctgg acgccgccga ggcggtgttg atcgaggcgt cgtccctgct cgacagcggc
     3361 gccagcgatg tgaatacgca gatcttcgcc gcaacctacc tcgcggaact ggcgctggct
     3421 cgccacgaca tcgaactggc gcaccggtac cgggacgagt tcctgcagct cgcggactac
     3481 cggggaaagc aaaccaacca cgcgctgccc agagtgttga tcgtcgccgc acgcatcgcg
     3541 cttgccgagg gcgctgctgc cgatgcggag cgttttgccc gtgacgggtt gcgccttgcg
     3601 cagaggaaat cacgcggccc ggatacgagc gcggatgtgg gtgaggcgtt gctgcgaatt
     3661 tcccaggcgc gacttctcgc gaaccctggc gccgatgtgg gatccatgct cgggcgggcg
     3721 gaccggtgtc tgaccgcggc gctcgacgcc aaccatccac tgacgatcga ggtacgaaaa
     3781 ctagggaaaa gaccgggcgc aatcggacca agggcggaca tcggttctgc cgccggcgtt
     3841 ccatgatccg ggcaatctca accgggcaag gggcgaagcc atgaaggcca ggatctcacc
     3901 attgatgttc atagcgacgc tgctgctggt ggccgcgact gcatctgcag acgaattcga
     3961 cgatacggtc tcgatcttca agaacgcggg cgagagcggc acgtacttcg ccaagagtta
     4021 tggctacgcg gtgttcccga ccgtgggtaa aggcggtctt ggcgtgggcg cggctcacgg
     4081 atcagggcgc gtcttcgcgc aaggcaaaca cgtgggcgac gtgaagatga accagatcag
     4141 tgtcgggctg caggccggcg gccaggcatt cagccagatc gtcttcttcc aggacaagcg
     4201 ttccttcgac gaattcacga gcggcagcta cgagttcgac gcgaccatgc aggcggtcgc
     4261 gatcaccgct tcagcgactg ccaccgccgg aacggcagga gctgcggcgg gtgccgccgg
     4321 cggaaagaag gacgcggcga cgacaggtaa ctaccacaag ggattggcgg tattcaccgt
     4381 cgccaagggc ggattgatgt accaggccgc cgtcggcggg cagaagttca agtacgaggc
     4441 gaagaagtag ctagacgccg gacagcaaaa agccgccgga acgttcgtcc ggcggccttg
     4501 ttctattctt cgcaaccgcg aatggtgctc ggcgtcaacc ctttgccttg aacgcgcgga
     4561 cctgcaggtt gaagcggtcg gccaccttca cgagttcctt ctgcgcggac tcgatcttcg
     4621 tgccgctctt ctcgcaggcc agatactcct ccatcgaacc cttgaacgcg tcgacggcct
     4681 tcttggcggc cagcatgtct tccttggacg cggacgcgcc gtcgggaatc gacgtcgggg
     4741 cggtgggcct gtcacagccc aatgccggca ccacggccac ggcccacaga actgcaatcg
     4801 ggaggatcct cttcatttca cttttctcca gttaactaaa catcgccacg gccatcgcgg
     4861 tcgcgtggac ggtcagctgg gttatcggca cggcgccgcg gatgcttgag gtccgaaagc
     4921 gctgcgaaat gtgaagcgcg tcctccatta ggtcatcggg cggccgatag cgaacccgtg
     4981 gcacgccgga aatcgcggga acccccgctc gatggcgcca tcgcgtcgat gcagcacgat
     5041 aaagaatcga gggagaaggc cggcgcttcg ggtgacggca tacgaaaggt attgaccttc
     5101 tcgctgggcg gggaggtcta cggcgttgac atccttcgcg tgaaagagat tcgcggctgg
     5161 tcgcccgtca cccggatacc gcaaacgccg gcctccatac tcggcgtgct gaaccttcgc
     5221 ggcctcatcg tgcccatcgt cgatctgcgc gtacgcttcg cgctcaagac cgcggaattc
     5281 acaccgctca cggtgatcat cgttttgtcg ctgcgcaacg agagcggaca gcgcgagtgc
     5341 ggcatcgtcg tcgacaacgt aaatgacgtg gtcgacatcg caaccgacag catcaagcct
     5401 gcgcctgaac tcggcggcgg acagaccggc gaatacatcg aaggcatcac gtcccacgaa
     5461 gagcagatgt tgatactgct caacgcggaa agcgtggctt ccccggacct gtcagcgacc
     5521 gtgctcgccg aacaggccgc ctgagaaaca cgacacttta tccacactcg actattcgaa
     5581 cggaatttcg tatgcgcctc aatgtgttca aaaacattcg tctctcgttg cagctgtacg
     5641 cactggttgc gttcacgctg gtcatcgcga cggggctcat cttttatgcg atggagcagg
     5701 tgcgcagcac ccagggcaca ctcaagcaca ccatcgacaa ccgcatggtg tcggggcagt
     5761 cgatccaggg cgtcgccgac gcgctgtcgc tgtcaattga ggcgtcgctg gacgtcatcg
     5821 aaaagaagca gtccgcactc gatgcgcacg atcagatcaa gaaggccatc gacaaggcgc
     5881 gcgacgactg ggacaactat tttctgggcg acatgatccc ggaagaacag gaactcgccg
     5941 acgagaccac gccgatgctc gacggcgcgt acggcaagat cgacaagctg ctcaagaagc
     6001 tcgagagcaa cgaactggcg gatctgcccg cctggcgtaa cgagaccttg cggccggcgt
     6061 tggccgaagg ctccagcaac ctgaagaagt tgatcgccat gcagctgacg gcggccaacc
     6121 tggaccttgc cagggcaaac accaactacc agcaggcggt gcgcaacagc atcacgctga
     6181 tgtcgggtgg cgccttcctg gccatcgttc tcgcgtggat catcattcgc agcgcgatgc
     6241 gcaagctcgg cgccgatccg ggcagggctg cgcaggtggc gcgacgtgtc gcctccggcg
     6301 acctcgagtt cgagatgctg cccggccgtc acgacgccat cagtctcatg ggtgcgctgc
     6361 gccagatgaa ggacagcctg ctgcactcca agctcgacta ccagggacag atcaatgcga
     6421 tcgcgaaagt ccagggtgtc atcgaatgtt cgctggacgg cgacgtcgcc aatgccaacg
     6481 atattttcct gaatcttctc ggctacacgc cggacgatgt caaaggcaag aactactcgc
     6541 tgttcgtcga ctcccaggac tcggccgcca accagggact ctggaaggcg ctgaaaggtg
     6601 gcgagagcca gcagggcgag ttccgcgtca tggcccgtga caaacgcgag ctctggttcc
     6661 agggcgtgtt caatccgatc gtcgacgcct ccggcaaacc gttcaaggtc gtcgcctatc
     6721 taagcgacat caccagacaa cgtcaggaag cgttgctgaa tgcggcgttc cgcggcgcgc
     6781 tcgatcgcct cgacgccaac gtgatgtgtg ccgacaacga catgaagatc atcttcgtga
     6841 atcccgctgc cacccggatg ctggcgaggg cccaggagtc gttccgcgcg gacctgcctg
     6901 gcttcgatgc cggcaagatc atgggcgcga acctcgagtc gatgacacgc gaaccggccg
     6961 tgctgcgcgg cctgatcgaa cgcctcacgg atatcgtgaa ccgccaggaa gtcatcggcg
     7021 gccgcacgat gaaaacgatc atgagcccga tgaaggacga cactggccgt cgcctcggta
     7081 cggtgctcga gtggttcgat cgcacgcagg aagtcgccac ggaatccgaa ctgcaggacg
     7141 tcatcacggc ggtgacggcc ggaaatctcg acaatcgcat ctcgctcgaa ggcaaacgcg
     7201 atttcttcct gacgctgtcc cggggcatca acgaactggt ggaggcgatc gcgacggtgg
     7261 tcgtcgaagt gcgcggcgtg gtcgccgcgg ccaacacggg tgacctgacc cggcgcatcg
     7321 aaacccaggg caaggccggg ctgctcgttc agatcggctc tggcatcaac gatctcacca
     7381 gcagtctctc ggagctggtg tcgcaggtga agaaggcttc ggccgaagtg agtcgcggag
     7441 ccgacgaaat cagccagggc aataccaacc tgtcgcagcg cacggaagag caggcgtcgt
     7501 cgctcgagga aacggcatct tcgatggagg agatgaccag caccgtcaag cagaacgccg
     7561 acaacgcggc ccacgccagc aagctcggct acgccgcgcg cgatcaggcg gagaagggcg
     7621 gcgcggtagt ttcgaaagcc gttgccgcca tggtcggcat caacgatgcc tcgaagaaga
     7681 ttgcggacat catcagcgtc atcgacggca tcgctttcca gaccaatctg ctggcgctca
     7741 acgccgcggt ggaagccgcg cgcgccggtg agcagggtcg cggattcgcc gtggtcgcca
     7801 ccgaagtgcg caacctcgcg gggcgctcgg cgggcgcggc caaggagatc aaggcattga
     7861 tccaggacac cgtgagcaag gtgaacgaag gttcaaacct ggtgacgcag tccggaacga
     7921 cgctgggcga gatcgtgtcc gccgtgaaga aggtgacgga tatcgtcgcc gagatcgccg
     7981 cggcgtcgaa cgaacaggcc gcgggcatcg accaggtgaa caaggccgtg atgcagctcg
     8041 acgaactcac gcagcagaac gcggcgctgg tcgaagaggc gtcggcggcg agccaggcca
     8101 tggccgaaca ggcgcgcgac ctgaattcgt ccatgtcgcg ctaccaggtg aacgccacgg
     8161 cagccgaggc cgcggcgccg gaatcgacgg ttcctgccgc gccgacgctg gccgcggatc
     8221 ggcgcaaggc cacgcggccg tggaccgaga agcctgccgc ggctgccaag gcaccggcgg
     8281 cccgtgcgtc cgcgcgtgtc gccaacggcg ccgacgaaac ggaatggaag gagttctgaa
     8341 gcccgcgctt cggggttctg cgaattgtcg atagcgacct cgagggtatc agcggaagct
     8401 ggatgcctca tcaacgaggt atcgcaatgc aattcccccg ccggctccaa tgcgtcgttc
     8461 tgcttgcagg cgccctgcag gcactgactc cggtcgtgtc ggtcggcgcg gaagcgccgc
     8521 acagtgccgg aaggccgcgt gtcggcctgg tcctcgcggg cggcggtgcg aaaggaggcg
     8581 cacatgtcgg cgtgctgaaa gtactggaag aacttcacgt gccgatcgac tgcatcgccg
     8641 gcaccagcat gggcgcgctc atcggcggcg gttatgccgc cggaataccg gctgacgagc
     8701 tcgaaaaatt cctggtgaac atcgactgga agaaggtggt cggcagccag ggccgtcgcg
     8761 acctcgaacc catcgaacag aaacgttccg gtcccacgta cagcaatgac ttcgagctgg
     8821 gcatcacgcc gaacggagtc gttacccccg gcggcctcat caacaccagc aatatcgaag
     8881 atctgctgcg cgtgtatgtc gcgcgggcca ggctcgaaac cgacttcgac aagctaccga
     8941 ttccctaccg cgccgtggcg accgacatgg tctcgagcaa gatggtcgtg ctcgaacagg
     9001 gcgacctggc caccgcgatg cgcgccagca tggcgattcc aggcgggttc gcgccagtga
     9061 tcatggacga catgatcctc tcggacggcg gcctcgtgag gaacataccg atcgatatcg
     9121 cgcgcaatct gtgcgccgag caagtcatcg tcgtgaacct ggtcgagccc gaggcggatc
     9181 ccgtgaaact gcagtcggcc acgcagctgc tggggcggtc gatggacgtc atgatcatcg
     9241 cgaacgagga gctgcaactg gcgtcgctgc ggccaggcga tgtccgtatc gacgtcatca
     9301 tgggcgatat cggcaccgcg gatttcgagc gcgtgccgga caccgtgccg ctcggcgtga
     9361 ccgccgcgca caagatgtcc gacgaactca ggaagttctc cgtgcctgag gcgcagtacg
     9421 tcgcctggcg cacggccgtg acgcaatccc agcagatcga gggccggctt gccggcgtgc
     9481 gtttcgaagg actcaagcgc gtcaatcccg aatacctggt ccggcgacac caggtgaagg
     9541 cgggcgaccc ggtcgacatc gtgacgatca gcgaggaagc acagcgcatc tcggcgcttc
     9601 aggacttcga ttccgtcagt tatcgcctgg atggcgatcg ttcgtcgccg acccttacct
     9661 ggctgccgca ggaaaagaaa attggtccta actatctgcg ggtggatctc ggggtctacg
     9721 cttcccggga aggcgacctt tccttctcca tttatggccg gcatgtccgc acctgggtca
     9781 actcgctcgg ggcgcagtgg tacaacgaac tgcaactggg tggcgagagc ctggtgacga
     9841 cgggctttta ccagccactc gatgtcgcgc gccggttttt catcgagccg cgcgtcacct
     9901 tctcgcgcgc tctggaagac gtattcgtcg atgacgagcg cgtagcgcgc tacgagttca
     9961 aggatttcat cggggagctc aatctcggag cgaacgtggg tcgctacggg caggctcgca
    10021 tcggttacct gtacgacgaa cgcgacgtcg acgtggatat cggtacgccg ctcatgcccg
    10081 agggcaaacc cgtggatgcc ggcatgcacg tccttgccga gctcgacagc cgcgatacgg
    10141 cattcaatcc cacccgtgga ttggccatgg cgctcgaata catggaaagc gatgagtcga
    10201 tgggtggcga tcgggattgg gaacgggccg agctcggctt cggcattgcg ttacctctgc
    10261 gccgcgacgt gttgtggctg accttcgccg ccggatcgga cctgggcagc gacctgcctg
    10321 gcgatcgtgc gttttccgtg ggcggtcccg acagcttccc cggcttcgaa caaggcgaac
    10381 tgcgggtcag cgaatactgg ctcgccaaca cgagctacct ctggagagtg aaagatctgc
    10441 tctccatccg gaatcattcg ctctacgtgg gagtcggcct gacgggaggc caactcaacg
    10501 gccgactcga cggtggcgag gatggcgaac actacggcgg atcggtgttc ctcaccggcc
    10561 gcacgttctt cggtcccctg accgtcggcg tgggcgctac gtccactgat tcatggagcc
    10621 tgtggctgag cgtggggcgg cccgtcgggc acgggacgat cctggaaaga ggagtcttcc
    10681 gctagcgcat ttagttagtt cggcagcttg cgcggcgtgt acggccgcgc cagtccggcc
    10741 agccactgcg cgtcctgtcc gtagatgtcg cgacggaagt tcacgcgtcc ctgcggatcc
    10801 acccaggcag tccaataggc gagcagcacg ggcactttgg cgggcagcgt cacgttctgc
    10861 gtgttgcccg catcgatggt tttccggatc gtcccggcat tccacgtggc cgggttcgcc
    10921 agcagcaatt ccgcgagctc gagagggcgt tctacacgta cgcagccgga actgaaggct
    10981 cgatcagtct cctcgaactt cgcctgcgac ggcgtgtcgt gcagatagat cgaatacgaa
    11041 ttcggaaaca tgatcttcac gcggccgagg gcattcttgg gtcccggatc ctgccgcagc
    11101 gtatatggga tgtttccgct cttgaacttc gaccagtcga tgctcgccgg atcgatgatg
    11161 ttgccacgcg catccagcac attgaggccg cgtcgcgtga tcgacgcagg atcgcgccgg
    11221 gcatcgggga gaatgtcgtt gcggatgatc ccgggaggaa cggtccaggt ggggttgaac
    11281 accaggtagt tgatgtcgga gcggaacatc ggggtgcggc ggtaggtctt gccgacctgc
    11341 gcgcgcgcgc tccacacgat ttcctggccg cgcacgagat agacccagta cgcggcgata
    11401 ttcacgacca cgaactcgtt cggcaggtca tgcagcaaga cgcgcccgcg atcgaggttc
    11461 acgcgcagct ggctgatccg ctcgtcgaac gacacattca gctcggagat cgtgcgggcc
    11521 ccgatctttc catcggccac gagccccatc ctttcctgga atcgccgcac cgcgctctgc
    11581 agtgtcgggt cgtacagatc ggatgaggtg acttcggcgg catcgaggtc gccactgatc
    11641 gacagtcgct tgcgcagcgc cggaactcgt tcatcgtgcg cgccggggtc gatcgatttg
    11701 ccattgttcg tttcctgggt cagggcgacc cactcgccgc cggcggccgc ttcccgaaaa
    11761 cgcgccagct catgcttcag cgtccgatac atgcgatgcg tcggtttcag cttttcgatc
    11821 tcggcatgaa tgtcgtcgct ggcaatggcg agctcgagtc tttgcggaac gtcgatgccc
    11881 gcagggctgc gcgtgtagtt ccactgtgcg tcgaacgagg cggcatccac tttcccgaac
    11941 atgaggtgat cggcgatgcg cagcagcgca tcggtgtgca gtatctcgaa ggccgcctgc
    12001 gcggaagggc tgcccgcggc accggcggcg agttgctcca ggacggccag gtggtaatcg
    12061 cgcgggtcga gtccgtcggc ctcactgtcc ttgagcgccc ggcgcagttc gctggccgtg
    12121 tgtgcattgg tccacgcggg ctgaaacgag cgtctggtat agaacgcgta aatgacattc
    12181 ggataggcga cgtgcgcgcc gtgaatttcg aacggcccgg actcgagcat cgcctcaacc
    12241 tgggtttgca gctccgatga cgccgatgcc ggtgccggtg ccgagacgtc ctgggcgtgc
    12301 gccaatccgg cggtaatacc tgttatgaac aggagtgctt gcaaggcggc tgcggggagt
    12361 tttgacattt gcgccgatgt tgcgtgtttt gccctggaac cgagatgggc ctttcatctc
    12421 attgacccac gcgtgcaaga agtgagggca agaatccggc aatcgccgcc acaccgccca
    12481 gggacggcgc aatccgccct caattcaccg tagcgcaaga aaatttcagc gtctccatgt
    12541 gatgaccttc tccaatttgc cgcaatgcgc ctgttaactt ggacccatga aaacgaaacc
    12601 tgcgaaacat tttctcctcg cgtctgattt cgatcagacg ctgagcttca acgactcggg
    12661 attcgagttg agcgaattgc tcggagttac cagcttcgag cggaaggtcg ccggcctgtc
    12721 ggccagccat ctcgtgcaac agggcggtga gctcgcctat ctcatccgtc acgacccgga
    12781 attccgcggc gtacgccgcg agcacctcat cgaggccggc aagcgggtac gtctcaagca
    12841 gggtctgccg gaattcgtcg aaatcctcga gcgcggcatc gatggcaacc agttctcttt
    12901 ctacgtggtc tcggcggcgc cccgcgaagt cgtcgtgtcc gctctcgaag gcatcgtgcc
    12961 gccggaacac atctatggca cggaactcga ctacgacccg cattcgggcg aggtgcgttc
    13021 gatcaaccgc gttcgtgccg gctacggcaa ggtggcggtg ctcgacgagc tggggcagaa
    13081 gctcgggatc accaccgatc gcgtgatcta cgtcggcgac ggcagctccg acattcacgt
    13141 gatgctccat gtgaacaatg gcgatggctt cacgatcgcc gtttccgaga accggcagct
    13201 cgcgcgcatc gcgcagagca cggtgctcag cgacaacgcg agcagcgtgc tggtaccggt
    13261 gctcgaccag gtgctggggc ttcgacccgg ctatatccgc gcccagctcg aatcgactgg
    13321 cctggtgctc gatgactggc agcgcgcccg aacggatcgc gtgttgattc gggaagcggc
    13381 gccgctggcg tccgcgggtt gagcgccgcc atgactgaca tgctcggttg ggtcgccacg
    13441 gccgtattcg tcggctcgta ctttttctcg cgtccggcgc ttttgcgcgc ggcgcagatg
    13501 gtgggtgcga cgctgtggat cgtctatggc gccttgatag gcgcgatacc ggtggtggtc
    13561 gccaacggcc tggtattcgc cgcggccgcc tggacgttgt ttcgcgcgag accgccgctg
    13621 actcagacct gaagttccgg cctgacgccg tccgcgcacg tgccgtacga ttcgcggcat
    13681 gactgacatc accctcatag gccctggcgc aatcggctcg acgctggccg cgtggctttc
    13741 gcagaatcgc gatcaccagg tcacggttgc cgcaagaaca ccgttcgatt ccatcgtgct
    13801 cgagacgccg cgtggcactc tcaatgcgtc gccccgcgtg ctcaccgatc cttcgcaggc
    13861 gcgtccggcg gattgggtgc tcgtcacgac caaggcctat gattcggacg ccgcggcgcg
    13921 ctggttcgag aagagttttg gcccgcgaac gcgcctcgcc gtgatccaga acggtgtcga
    13981 acacgtcgaa cgctttgttc gctacgtcgc tgcggatcgc atcgtgccgg tcatgatcga
    14041 ttgcccggcg acgcgtcatt cacctggccg cgtgacgcag ggcgggccca tccacatcgt
    14101 cgtacccgcg ggcgtcaacg gcgcggcttt cgtcgaccag ttcgcggggc tcagtctcga
    14161 cgtgcgggaa acaccggact tcatcaccgc ggtctggaag aagctttgtc tcaacagtgc
    14221 aggggcggtt tcaaccgcat tgctcgcgcc cgccggcatc gcgcatcacg atggcgtcgc
    14281 agaaattctt cgtgccatcg tgcgtgagtg cattgccgtg ggtcgcgcca ggggcgccgt
    14341 tctcggcgac gagatcgccg gggaaatcgt cgaccgcatg cgcagcgggc cgcgcgacgc
    14401 cgccaactcg atgctcgccg accggcgcgc cgggcggccc atggaaatcg atgcgcgcaa
    14461 cggggtcatc gtccggtacg gacgcgagca tggcattccg acgccgatga atgcgctcat
    14521 ggtggccatg ctcgaggccg tcgatcaatg tagctagtcc gccaggccgc ctatccagct
    14581 gcgcaggacg ctgagttcct catccacgag caccaggtcg gcgcgctggc cgggggcgat
    14641 gcggcccatc cgaccgtcca gcttcaggaa cccggccggg ttggtgctgg ccatgcgcag
    14701 cgcgtcggtc aacgacacgc ccagctggtt caccgtgttg cgcaccgcgc tcgccatgtc
    14761 gagatgtgcg cccgccagcc ggccttcgtc gtcgatgatc cggtcgccgt cgaccgtgat
    14821 gatgcggccc tggatccgga attccttgac gtccgcgccc acgctcggca tggcgtcgct
    14881 caccagcatg aagcggtcgt gccgtttggc gcgcagcgca atcttcagca cttccgcatg
//
LOCUS       DASQJS010000253         9238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1398854, whole genome shotgun sequence.
ACCESSION   DASQJS010000253 DASQJS010000000
VERSION     DASQJS010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398854"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VEW08_10235"
     CDS             <1..304
                     /locus_tag="VEW08_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HYJ41161.1"
                     /translation="FTNGISQPLGQIAIANFANVQGLQQLGNTQWAETFASGQALRGQ
                     AGNSGFGLVQSGALEGANVDVTEQLVNMITAQRNFQANAQMISTADSITQTIINMR"
     gene            326..1066
                     /gene="flgF"
                     /locus_tag="VEW08_10240"
     CDS             326..1066
                     /gene="flgF"
                     /locus_tag="VEW08_10240"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HYJ41162.1"
                     /translation="MDRMLYVAMTGAKETLNAQAVNNHNLANASTTGFRAELSAFQSR
                     AVDGSGYASRVYATQASTGWNNQSGALLSTGRDLDVAINGDGWIAVQGPDGKEAYTRA
                     GDLQVDANGQLTTGTGLPVLGDGGPITVPPYTSIFFARDGSISVVAQGQTPDTTATVD
                     RIKLVKPADEAIERGSDGLFRMKDGTEAAPDASVQLGSGTLESSNVNTADAMVTMIEL
                     ARHFEMQVKAIRHAEENGAAAAQIMRLG"
     gene            1081..1866
                     /gene="flgG"
                     /locus_tag="VEW08_10245"
     CDS             1081..1866
                     /gene="flgG"
                     /locus_tag="VEW08_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017130126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HYJ41163.1"
                     /translation="MNPALWAAKTGLDAQQTRLAVTSNNLANVSTSGFKKSRAVFEDL
                     LYQNVRQVGASTSQDTQAPSGVHLGTGVRVVATEKIYTQGSLSQTGNALDVAIEGRGF
                     FQIQMPDGTTTYTRDGSFQLSAQGQMVTSSGYILQPGISIPDGSQSITIGRDGVVTVR
                     VAGQGNPVQVGQLQLTDFVNPAGLQPLGENLMAETTASGPAQTGTPGQNGLGPVSQGA
                     VEASNVNVVEELVNMIETQRAYEMNSKAISTADQMLEYVNNQL"
     gene            1869..2549
                     /locus_tag="VEW08_10250"
     CDS             1869..2549
                     /locus_tag="VEW08_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HYJ41164.1"
                     /translation="MKIVHVVLLSLFHCFILGGCASMQEDGGRDFEPSWPDDADVPKA
                     LPGTIYAQGRDVTLWNNVTARNVGDTLTIRLAESTEAEKSATTTASKSSKATLTGPTI
                     AGAPVTVNGVPVLEAGMDNSQDFAGNGAATQSNKLDGFITVTVYKRLSNGNLVVRGQK
                     WVAINSGREFVRLQGIVRPSDIAPDNSVLSWRVADAYISYGGQGTVANASKPGWLFRF
                     FNSPKTPF"
     gene            2567..3673
                     /locus_tag="VEW08_10255"
     CDS             2567..3673
                     /locus_tag="VEW08_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HYJ41165.1"
                     /translation="MRLSMAAFAAAISLIAASAAQAERVKDLASVQGVRQNQLVGYGL
                     VVGLAGTGDQTSQAPFTIQSIKNMLIRYGITIPANTNPQLKNVAAVTVHADLPPFAKT
                     GQNIDVTVSSIGNAQSLRGGSLIMTPLIGADGEVYAIAQGSLVVGGFGVSGKDGSSIA
                     VNVPSAGRVPNGATVEREVPNGFTSEPFVMLNLHTPDFTTAARLTEGINKLLGEGTAH
                     SVDSVSVKVTAPADASQRTAYLATLEAIEIEPGDAPARVIINSRTGTVVIGSHVRVMP
                     AAVSHGSLTVTITERASVSQPNEFGRGDTVVVPESSISISQPPARMFVFDAGVDLNDI
                     VKAVNQVGAAPGDLVAILEALKESGALRAELIVI"
     gene            3683..4606
                     /locus_tag="VEW08_10260"
     CDS             3683..4606
                     /locus_tag="VEW08_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HYJ41166.1"
                     /translation="MALSIDPNNVPAARGADTPRVDANDFKQFAALRRGAEANDPATL
                     RAVAKQFESLFTKMMLESMRSASMGDPMFGSDQGDMYQDMADDQLAVQLSEGRGLGLA
                     DMLIRQLSGGAGGGTNAIKPATDTDAAEVATPEQREQFISGLMPHAEAAARELGVDPN
                     NLIAQAALETGWGRSQPGGNSNNLFGIKAGESWNGHSVQANTEEFAGGVANRVDASFR
                     AYGSPRESVEDYVRLIRDNPRYAGAVNTGSDVQAFANALQRGGYATDPDYARKLGAVA
                     AEVRQRIAVNQFKSESAAPIAGQDPLRMNNG"
     gene            4599..6488
                     /gene="flgK"
                     /locus_tag="VEW08_10265"
     CDS             4599..6488
                     /gene="flgK"
                     /locus_tag="VEW08_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HYJ41167.1"
                     /translation="MADVLSTGLSSLRSLQRALDTTSHNIANVSTEGYTRQRVEFQTR
                     RPEMSGGGWVGTGVETSTVRRVYDQFLAQQTRSSGTNLARLDAFASQAARIDNLLGDS
                     ANGLSSSLQSFSDAINEVSTTPASIPARQVLIAEGRALAERLRSYDARMRDMSAEVNG
                     RLSVEAQEITTLAAGIAKLNGDISVAVQQSGQPPNDLLDQRDALINELSGKVGVSVVA
                     EGDSMLNVFIGNGQPLVLGNLASQITTTVDPLDPERTLLSMRTGAGTVDLSRVVSGGT
                     LGGLLDWRSQMLEPARNELGRIGVAIAGQVNAQHREGMDLTGAMGGNFFNIGAATATP
                     ATANTGTAAVTVTRTNLGAITPNDLVLSRTATGYTLRRQDTGAAVSFTGTGTGADPIV
                     ADGLSIVVGAGIATGDQYTIRPTRDALPGMSVAITDAARVAAAAPIRASAATTNSGTG
                     TVSSGEVLDPTNAALLTTVNIDFTSATTYTVNGAGSFTYTPGSAIDVNGWRIQVNGAP
                     AVGDRFTVRNNAGATGDNRNAFALADALRANVLDGGTKSAFSGVEKLIADVALSTRTA
                     QVNRDAEAVIHDSDVAALDSVSGVNLDEEAANMLRYQQAYAAAAQIISVANEMFDTLL
                     NAVRR"
     gene            6494..7699
                     /gene="flgL"
                     /locus_tag="VEW08_10270"
     CDS             6494..7699
                     /gene="flgL"
                     /locus_tag="VEW08_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HYJ41168.1"
                     /translation="MRLSTAGMHRTSINAILEQQARMAKTQEQVTTGKRFQSASEDPI
                     ASARAAVLDRSVAENEQFSRNSNIVEARLNFEEQSLADATAVLQYVRERALQGANSTL
                     GADERRMLANDVRQQLQALVEIANRDDGNGEYLFAGTSTGAAPFAQGTTGVNYLGDMT
                     NRVVRVSNSQSIADGHSGADVFMNLAERNGVFRTDVTATNTGSGTIDVGRVVNSTQWV
                     PNNYSLQFTTATDWQVVDDTLPTPNVIASGAGFVSGQSISFLGANVTITGTPAAGDSF
                     SIAPAQKTDMFAMLEELAVTLGSATTTPIDKATFQAQIGASIANLDQSLSRASLVRAE
                     VGSRLSAIDLAADTRGAESVDLQSLLADLRDVDYAEAVSRLNQEYTGLQAAQAAYTKI
                     AQLSLFDYL"
     gene            7836..>9238
                     /locus_tag="VEW08_10275"
     CDS             7836..>9238
                     /locus_tag="VEW08_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin hook IN motif-containing protein"
                     /protein_id="HYJ41169.1"
                     /translation="MPQVINTNVASLTAQRNLTTSQGQLATALQRLSSGLRINSAKDD
                     AAGLAISERFTTQIRGLNQAVRNANDGISLAQTAEGALGETGNALQRIRELAIQSANS
                     TNSASDRAALNAESAQLLAEIQRNGLATQFNGQNILDGSFSSAQFQVGANANQTISFG
                     ITGATTNLLGAYQATSQAVSSAAFDGNSFTVNGVEVGVSAQTSAAGVTADSATAKATA
                     INSKTNLTGVSATATTSVQGAVGPVARSGLANGELVINGIAVGSVAGNASAVTQGRSA
                     ATAINAVSNQTGVSASADASTGRLTLTAADGRNIELTSGSGDADGATAIYNATGLNVT
                     DTVVPTGHDTFTMTIAGAFDADGGTAISLAHGDTLELDGVTYEFVAAAANVTSGNVAV
                     VVADAATAAATGTALRTAIQGQYTLGNTSLVATGTNAILLVNDKLGANGTVAEYLDES
                     GITHGGDATAVTVSALSG"
BASE COUNT         1780 a         3101 c         2900 g         1457 t
ORIGIN      
        1 cttcaccaac ggcatctcgc agccgctcgg ccagatcgcc atcgcgaact tcgccaacgt
       61 gcagggcctg cagcagctcg gcaacacgca gtgggccgag acgttcgcgt cgggccaggc
      121 gctgcgcggc caggccggca attccggctt cggcctggtg cagtcgggtg cgctcgaagg
      181 cgccaacgtc gacgtgaccg agcagctggt gaacatgatc accgcgcagc gcaacttcca
      241 ggcgaacgcg cagatgatca gcaccgccga ttcgatcacc cagaccatca tcaacatgcg
      301 ctaacgcgca tacaggtagc tagttatgga tcgcatgctc tacgtggcga tgaccggcgc
      361 gaaggaaacc ctcaacgcgc aggccgtcaa caaccacaac ctcgccaacg cgagcaccac
      421 cggatttcgt gccgagttgt ccgcgttcca gagccgtgcg gtcgatggtt ccggctacgc
      481 atcgcgcgtt tacgccaccc aggcgtcgac cggctggaac aaccagtcgg gcgccttgtt
      541 atcgaccggc cgcgacctcg acgtcgcaat caacggcgac ggctggatcg cagtacaggg
      601 ccccgacggc aaggaagcct acacgcgcgc cggcgacctg caggtggacg ccaacggcca
      661 gctcacgacc ggcacgggcc tccccgtgct tggcgacggc gggccgatca cggtgccgcc
      721 atacacctcc atcttcttcg cgcgcgacgg atccatttcg gtggtggccc agggccagac
      781 gccggatacc acggcgacgg tggatcgcat caagctggtc aagcccgccg atgaagcgat
      841 cgaacgcggc agcgacggcc tgttccgcat gaaggacggc accgaagcgg cgccggacgc
      901 cagcgtgcag ctgggctcgg gcacgctcga gtcgagcaac gtcaacaccg ccgatgccat
      961 ggtcaccatg atcgaactgg cgcggcactt cgaaatgcag gtcaaggcga tccgccatgc
     1021 ggaagagaac ggcgccgccg ccgcgcagat catgcgtctc ggttagtcag gagtaattaa
     1081 atgaatccag ctttatgggc agccaagacc ggactcgatg cgcagcagac gcgcctcgcg
     1141 gttacgtcca acaacctcgc caacgtgagc accagcggct tcaagaagag ccgcgcggtg
     1201 ttcgaagacc tgctgtatca gaacgtgcgc caggtcggcg cctcgacttc gcaggacacg
     1261 caggcgccgt cgggtgtgca cctcggcacc ggcgtgcgcg tcgtcgccac cgaaaagatc
     1321 tacacgcagg gcagtctctc gcagaccggc aatgcgctcg acgtggccat cgaaggccgc
     1381 ggcttcttcc agatccagat gcccgatggc accaccacct acacgcgtga cggcagtttc
     1441 cagttgtcgg cgcagggcca gatggtgacc tcgagcggct acatcctgca gccgggcatc
     1501 agcattcccg atggctcgca gagcatcacc atcggccgcg atggcgtggt gacggtgcgt
     1561 gtcgccggcc aggggaaccc ggtgcaggtg ggacaactgc agctcacgga tttcgtgaac
     1621 ccggccggcc tgcagccgct cggcgaaaat ctcatggcgg agaccacggc cagtggcccc
     1681 gcgcagaccg gtacgccagg ccagaacggt ctcggcccgg tctcgcaggg tgcggtcgaa
     1741 gcctcgaacg tcaacgtggt cgaagagctg gtcaacatga tcgagacgca gcgcgcctac
     1801 gaaatgaatt cgaaggccat ctcgaccgcc gaccagatgc tcgaatacgt caacaatcag
     1861 ctgtaatcat gaaaatcgtc cacgtagttc tgctctcgct ctttcactgt ttcattctcg
     1921 gcggctgcgc ctcgatgcag gaggatggcg gccgggactt cgagccgagc tggcccgacg
     1981 atgccgacgt gcccaaggcg ctgcccggca ctatctacgc gcagggcagg gacgtaacgc
     2041 tgtggaacaa cgtcacggcg cgcaatgtcg gcgacacgct caccatccgg ctcgcggaaa
     2101 gcacggaagc cgagaagagt gcgacgacca ccgcgagcaa gtcttcgaaa gcgacgctca
     2161 ccgggccgac catcgctgga gcaccggtca cagtcaacgg cgttcccgta ctcgaagccg
     2221 gcatggacaa cagccaggat ttcgccggca acggcgcggc aacccagagc aacaagctcg
     2281 atgggttcat caccgtcacg gtctacaagc gactttccaa cggaaacctg gtggtgcgcg
     2341 ggcagaagtg ggtagccatc aattccggac gtgaattcgt gcgcctgcag ggcatcgtgc
     2401 ggccgagcga catcgcgccg gacaacagtg tgctgtcgtg gcgcgtggcc gacgcctaca
     2461 tctcttatgg tgggcagggc acggtggcga acgcctccaa gcccggctgg ctcttccgct
     2521 tcttcaactc gccgaaaacg cctttctgaa caggaaacac aacaccatgc gcctatccat
     2581 ggcggctttt gccgccgcaa tttccctgat cgccgcctcg gccgcccagg ccgaacgcgt
     2641 caaggatctc gcgtcggtgc agggtgtgcg ccagaaccag ctcgtgggtt acggcctggt
     2701 tgtgggtctc gccggtaccg gcgaccagac cagccaggcg ccgttcacca tccagagcat
     2761 caagaacatg ctgatccgct acgggatcac cattccggcg aacaccaacc cgcagctcaa
     2821 gaacgtcgcg gcggttaccg tgcacgccga cctgccgccg ttcgccaaga ccggccagaa
     2881 catcgacgtc accgtgtcgt ccatcggcaa cgcgcaaagc ctgcgcggcg gtagtttgat
     2941 catgacgccg ctgatcggcg ccgacggcga ggtgtacgcc atcgcgcagg gcagcctggt
     3001 ggtcggcggc ttcggcgtct cgggcaagga cggctcgagc atcgcggtca acgtaccgtc
     3061 ggccgggcgc gtgccgaatg gcgcgaccgt cgagcgcgaa gttccgaacg gtttcacctc
     3121 cgagcctttc gtgatgctca acctgcacac accggatttc accaccgcgg cgcggctcac
     3181 cgaaggcatc aacaaattgc tgggcgaggg caccgcacac tcggtcgaca gcgtgtcggt
     3241 caaggtgacg gcaccggccg atgccagcca gcgcaccgcg tacctggcga cgctcgaggc
     3301 gattgaaatc gagccgggcg atgcgccggc gcgggtcatc atcaattcac gcactggcac
     3361 cgtggtcatc ggctcgcatg tgcgcgtgat gccggctgcc gtgtcccacg gttcgctcac
     3421 cgtgaccatc accgagcgcg cctcggtgag ccagcccaat gaattcggcc gcggcgacac
     3481 cgtcgtggtg ccggagagct cgatctcgat ctcgcagccg ccggcgcgca tgttcgtatt
     3541 cgacgccggt gtcgatctca acgacatcgt gaaggctgtc aaccaggtgg gcgccgcgcc
     3601 cggcgacctg gttgcaattc ttgaagcact caaagagtcc ggcgcgctgc gcgccgagct
     3661 catcgtcatc tgaagacgcg ccatggcact gagcatcgat cctaacaatg taccggctgc
     3721 acggggcgcg gacactccgc gtgtcgacgc caacgatttc aagcaattcg cggccctgcg
     3781 ccgcggcgcc gaagccaacg atccggcgac gctgcgtgcc gttgccaaac aattcgagag
     3841 tctgttcacc aagatgatgc tcgagagcat gcgcagcgcc agcatgggcg atccgatgtt
     3901 cggcagcgat cagggtgaca tgtaccagga catggccgac gaccagctcg ccgtgcagtt
     3961 gagcgaaggg cgcggcctgg gccttgccga catgctcatc cggcagttga gcggcggggc
     4021 gggcgggggg actaacgcca tcaagccggc gaccgatacc gatgcggcag aagtcgcgac
     4081 gccagagcag cgtgaacaat tcatctccgg gctcatgccg cacgccgagg ctgcggcgcg
     4141 cgagctcggc gttgatccca acaacctcat tgcgcaggcc gcgctcgaaa cgggttgggg
     4201 gcgttcgcag cccggtggca acagcaacaa tctcttcggc atcaaggccg gcgagagctg
     4261 gaatggccac agcgttcagg caaacacgga agagtttgcc ggaggcgtgg ctaaccgcgt
     4321 cgacgcgagc tttcgcgctt acgggtctcc ccgagagagt gtcgaagatt atgtccgcct
     4381 tattcgcgac aacccgcgct acgcgggggc cgtgaacacc ggcagtgacg tccaggcctt
     4441 cgcgaatgcg ctgcaacgcg gcggttacgc cacggatccg gactatgcgc ggaagctcgg
     4501 ggcagtcgcc gcggaagtgc ggcaacgaat tgccgtgaac cagttcaagt cggagtccgc
     4561 ggcgccgata gcggggcagg acccgttgag gatgaacaat ggctgatgtg ttaagcacgg
     4621 ggctatcgag tttgcgttcg ttacagcggg cgctcgatac cacctctcac aacatcgcga
     4681 acgtttcgac ggagggttac acccgccagc gtgttgaatt ccagacgcgc aggcccgaaa
     4741 tgtccggcgg tggctgggtc ggcacgggcg tcgagacttc gaccgtgcgt cgtgtttacg
     4801 accagtttct cgcgcagcag acccgaagca gcggtaccaa cctcgcgcgg ctcgacgcgt
     4861 tcgcctccca ggccgcacgc atcgacaacc tgttaggcga ttcggccaac ggattgtcga
     4921 gctcgctgca gagcttcagc gacgccataa acgaagtttc cacgacgccg gcatcgatcc
     4981 cggcgcgcca ggtgctgatc gccgaagggc gcgcgctggc cgaacgcctc aggagttacg
     5041 acgcgcgcat gcgcgacatg tcggccgagg tcaacggccg gctcagcgtc gaagcgcagg
     5101 aaatcaccac gctcgcggcg ggcatcgcca agctcaacgg agacatttcc gttgccgtcc
     5161 agcaaagtgg ccagccgccc aacgacctgc tcgaccagcg cgatgcgttg atcaatgaac
     5221 tgtctggcaa ggtgggtgtg tcggtcgtcg ccgaaggcga ttccatgctc aacgtattca
     5281 tcggcaatgg ccagccgctg gtgctcggca acctggcgtc gcagatcacc acgacggtgg
     5341 atccgctcga tccggaacgt acattgctgt ccatgcgcac gggcgcgggc accgtcgacc
     5401 tgtcacgcgt ggtgtccggc ggaacgctcg gcggcctgct cgactggcgc agccagatgc
     5461 tcgagccggc gcgcaacgaa ctcggccgca tcggcgtcgc catcgccggc caggtcaacg
     5521 cgcagcatcg cgagggcatg gacctcacgg gcgcgatggg cggcaacttc ttcaacatcg
     5581 gggcggccac cgcgacgcct gccacggcca acaccggcac ggcggcggtc acggtcacgc
     5641 gtacgaatct cggcgcgatc actcccaacg acctggtcct ctcgcgcacc gccactggct
     5701 acacgctgcg ccgccaggat accggcgcgg ccgtcagctt caccggcacg ggcaccggcg
     5761 cggatccgat cgtcgccgat ggcttgtcca tcgtggtggg ggcgggcatc gcgaccggcg
     5821 atcagtacac gattcgcccg acgcgcgacg cactcccggg catgagcgtg gcgatcaccg
     5881 acgcggcgcg cgtcgccgcc gccgcgccga tccgcgcctc ggcggccacc acgaactccg
     5941 gcaccggaac cgtgagctcg ggcgaagtac tcgacccgac caacgcggca ttgctgacca
     6001 cggtcaacat cgatttcact tcggcgacca cctacaccgt gaacggtgcg ggcagcttca
     6061 cgtacacgcc gggttcggcc atcgacgtca acggctggcg catccaggtc aatggcgcgc
     6121 ccgcggtcgg cgatcgcttc accgtgcgca acaacgccgg cgccaccggc gacaatcgca
     6181 acgcattcgc actggccgac gcgctgcgcg ccaacgtgct cgacggcggc acgaaatccg
     6241 ccttctcggg tgtcgagaaa ctgatcgccg acgtggcgct ctccacgcgc acggcgcagg
     6301 tcaatcgcga tgccgaggcc gtgatccacg acagcgatgt cgctgccctg gactccgtgt
     6361 cgggcgtcaa tctcgatgag gaggcggcca atatgctgcg ctaccagcag gcatatgccg
     6421 ccgccgcgca gatcatttcc gtagccaacg aaatgttcga cacgctgttg aacgcggtcc
     6481 ggagataact cacatgcgcc tgtccaccgc cggcatgcat cgcaccagca tcaacgccat
     6541 cctcgagcag caggcccgga tggcaaagac gcaggagcag gtcaccacgg gcaagcgatt
     6601 ccagtccgcg tccgaggatc cgatcgcttc ggcgcgcgcc gccgtgctcg accgttcggt
     6661 ggccgagaac gaacagttct cgcgcaactc gaacatcgtc gaagcgcgcc tgaatttcga
     6721 agagcagtcg ctggccgacg ccaccgcggt gctgcaatac gtgcgcgagc gtgcgctgca
     6781 gggcgccaat tcgacgctcg gcgccgacga acgccgcatg ctggccaacg acgtgcgcca
     6841 gcagctccag gcgctggtcg agatcgcgaa tcgcgacgac ggcaacggcg agtacctgtt
     6901 cgccggcacg tcgaccggtg ccgcgccatt cgcgcagggc accaccggcg tgaactatct
     6961 gggcgacatg actaatcgcg tggttcgcgt gagcaacagc cagtcgattg ccgacggcca
     7021 cagcggcgcc gatgtgttca tgaatcttgc agaacgcaac ggggtgttcc gcacggatgt
     7081 gacggccacg aataccggca gcggcaccat cgatgtcggt cgcgtcgtga attccacgca
     7141 gtgggttccg aacaactatt ccttgcagtt cacgaccgcc accgactggc aggtcgtcga
     7201 cgatacgctg cctacgccga atgtcattgc gtccggcgcc ggctttgtca gcggacagag
     7261 catttcattt ctcggtgcga acgtgaccat cacgggcacg ccggccgcgg gcgacagctt
     7321 ttccattgcg cccgcgcaga agaccgacat gttcgcgatg ctcgaagagc tcgccgtgac
     7381 gctcggttcc gcgaccacga caccgatcga caaggccacg ttccaggcgc agatcggcgc
     7441 gagcatcgcg aatctcgatc agagtctctc gcgcgcatcg ctggtccgcg ccgaagttgg
     7501 ttcgcgcctg agcgccatcg acctggccgc ggacacacgt ggcgcggagt ccgtcgacct
     7561 gcagtcgttg ctggcggatc ttcgcgatgt cgactacgcg gaggcggtca gccgtctcaa
     7621 tcaggaatac accggtcttc aggccgcgca ggcggcctat acgaagatcg cgcaactgtc
     7681 cttattcgac tacctgtaac taacaccgtg atatgcctca caaatatgtg cgggcccgct
     7741 actaacgctt aagttcgccc agacccaacc gatacaggcc ctgagcaaac tattgccggt
     7801 gttgttaccg gcccacaaat tcaggagtcg tttcaatgcc tcaagtcatc aacacaaacg
     7861 tggcgtcgct gactgctcag cgcaacctca cgacatcaca gggccagttg gcgacggcct
     7921 tgcagcgtct ctcttccggt ctgcgcatca acagcgcgaa agacgacgcc gccggtctcg
     7981 cgatctcgga gcgtttcacg acgcagatcc gtggtctgaa ccaggctgtc cgcaacgcga
     8041 atgacggtat ctcccttgcc cagaccgcgg aaggcgcgct cggcgaaacc ggcaacgccc
     8101 tgcagcgtat ccgcgaactg gcgattcagt ccgccaacag cacgaactcg gcctctgacc
     8161 gcgcggcgct caacgccgaa tccgctcagc tgctcgccga aattcagcgc aacggtctgg
     8221 ccacgcagtt caacggccag aacattctcg acggttcgtt ctcgtcggcg cagttccagg
     8281 tcggtgccaa cgcgaaccag acgatctcgt ttggtatcac cggtgccacc acgaacctgt
     8341 taggtgctta ccaggccacg agccaggccg tgtcgagcgc cgcgttcgac ggcaacagct
     8401 tcacggtcaa cggtgtggaa gtgggtgtct cggcccagac cagcgccgcc ggtgtcactg
     8461 ccgacagcgc cacggcgaaa gccacggcca tcaactcgaa gaccaacctg actggcgtca
     8521 gcgcgacggc cacgacttca gtgcagggcg cagttggccc ggttgcacgc agcggcctcg
     8581 cgaacggcga actcgtgatc aacgggatcg cggtcggctc agtcgccggc aacgccagcg
     8641 ccgtgaccca gggtcgcagc gccgccacgg ccatcaacgc cgtgtcgaac cagacgggcg
     8701 tcagcgcctc ggccgatgcc agcacgggtc gcctgaccct gacggcggct gacggtcgca
     8761 acatcgagct gacctcgggt tcgggcgatg ccgatggcgc cactgccatc tacaacgcca
     8821 cgggcctcaa tgtgaccgac accgttgtgc cgactggcca cgacacgttc acgatgacga
     8881 tcgccggtgc attcgatgcg gacggcggca cggcgatttc gctggcgcat ggtgacaccc
     8941 tcgaactcga cggtgtgacg tacgaattcg ttgccgcggc tgccaatgtc acttcaggca
     9001 atgtcgcagt tgtcgtggcg gacgccgcca cggcggcggc gaccggcacc gcgctgcgca
     9061 cggcgatcca gggtcagtac acgctcggca acacctcgct ggtggccacg ggcaccaacg
     9121 cgatcctgct ggtcaacgac aagctcggtg cgaacggtac ggttgctgaa tacctcgacg
     9181 agtcgggcat cacccacggc ggtgacgcta cggccgttac ggtcagcgcg ctctcggg
//
LOCUS       DASQJS010000254         6654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3294387, whole genome shotgun sequence.
ACCESSION   DASQJS010000254 DASQJS010000000
VERSION     DASQJS010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3294387"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VEW08_10280"
     CDS             <1..271
                     /locus_tag="VEW08_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41170.1"
                     /translation="RNWDMDSEIIKAIGACENIDREHAGAIDLTDVLTVSAALIALGP
                     APQSEQAPFPGMPAARRLGLDAEACAAALAESRDEIDALRQALNV"
     gene            268..1161
                     /locus_tag="VEW08_10285"
     CDS             268..1161
                     /locus_tag="VEW08_10285"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HYJ41171.1"
                     /translation="MSQAETTSTIESRAIPNTVVFGFVKALALELSQGRVDLPSVPDI
                     VLKMQRALADENVSNETVVKVLGSEPVLASKLLNMANSAALNTSGRKIADLRMAVARV
                     GFNIVRSAALSFAMEQLKSAPQFKHLEPMLDALWKHSVHVAALSHVVARRFTSLNGDT
                     ALLAGLMHNVGRIYILTRASGHPSLFADQLTYQSIVRDWHTNVAKALLENWQVADEIV
                     DAVSNHEDMNREVRGPVTLTDVVALASQLQQHTMQGALIDPTEPLVWALKRLQVKYAD
                     CQSVIDESASDIEALKTVLGG"
     gene            1168..2058
                     /locus_tag="VEW08_10290"
     CDS             1168..2058
                     /locus_tag="VEW08_10290"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HYJ41172.1"
                     /translation="MAQIALGTTTIENIVAHDAAALEKDLANTRADASVSLPGVPQIV
                     TRLQQLLSDPDVSIPQIVPVINYEPILVGRVLQMGNSAALNPTRRMVTDLKTAVTRVG
                     FTLLRSASLAYALRQVSQAHSVKDIRPHLEALWERSCWTAAVSYVIARKFTKVNRDLA
                     FLGGLMHGVGKLFILTRAAHYPFVLRDRTKYGAILRKWHASFAKGILTGWKVNDDVIE
                     AVGQYENLNREVIGDDPDLTDILATSYLVVGHTGRMQDLSMTVEKIASFSRLRLDLMA
                     VEETLEQAKEEIEDLRLAIK"
     gene            2175..3545
                     /locus_tag="VEW08_10295"
     CDS             2175..3545
                     /locus_tag="VEW08_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41173.1"
                     /translation="MQPENVRTAADARKIVEERKLDHVKVGVFDNDGIMRGKYMGRDK
                     FFSALDKGFGFCDVVLGWDSNDQLYDNVKYTGWHTAYPDAPVRVLPDTCRNVPFEGDM
                     VMFLGEFSEKAEAVCPRATLRRVLRKADDLGYLVNAATEFEFFMFRETPESIRAKGFR
                     DLTTMTPGFFGYSVLRSSVHSEFYHQLMNMCTGLRIPLEGLHTETGPGVLEAAIQYCE
                     ALEAADRGSLFKTFTKILAQRNGMMATFMAKWSKDWPGQSGHIHTSLVRKADGKAAFH
                     DTTKPHNMSDEMRWYVGGQQKLMPELLAMVACTVNSYSRLIPGFWAPTSATWGVENRT
                     TALRVIPGGASSQRVEYRIAAADINPYLALAVAIGSGLHGIENRIEPDPMVEGNAYDK
                     KFPKTRSLPRTLTEASEWLEKSRAGRALFGDAFVEHYAASREWEDREFRRAITDWELA
                     RYFEII"
     gene            3569..4969
                     /locus_tag="VEW08_10300"
     CDS             3569..4969
                     /locus_tag="VEW08_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYJ41174.1"
                     /translation="MNHSGIQKTISPVDGRVYVERPLATSAEINETLRTARHAFTEWR
                     NTSIADRAAILTRFCSEFEKRGSQIAEELTWQMGRPARYAPNEVRGTLERARHMIAIA
                     PQALGDVNTEPKDNFRRFVRREPLGVVFTVAAWNYPFLIAVNSVIPALMAGNVVVLKH
                     SAQTPLAAERFAECLQAAGLPKGAFQVLHASHEDTDHVIRDPRVDFVAFTGSVAGGRA
                     VQRAAAQRFIGVGLELGGCDPVYVRHDANLDHAIENIVDGAYFNSGQSCCGLQRIYVH
                     HSLYHKFTEGFVELTRKYSLGDPTDAATTLGPLVRTAAADGVRAQIQASIAAGATGAI
                     PEGSFARSVAGTPYLAPQVLLDVDHHMPVMREEIFGPVAGIMKVSSDDEAVDLMNDSD
                     FGLTAAIWTEDADAALAMGQRVNTGTWFMNRCDYLDPALAWVGVKDSGRGCTLSVVGY
                     EHLTRPKSFHMRIKTS"
     gene            4966..6132
                     /locus_tag="VEW08_10305"
     CDS             4966..6132
                     /locus_tag="VEW08_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HYJ41175.1"
                     /translation="MTIPMKGNWNYPTRIWSGPGRIAELPAACADLGIVRPLVVTDNG
                     LRDSQMIRRAVASLPGAGLFADVRGNPVAANVEAGLKAYHAGAHDGVVAFGGGSGLDA
                     GKVIAFMSGQTRPLWDFEDVGDWWTRADPKGIAPVVAVPTTAGTGSEVGRAGVILNEE
                     THQKKIIFHPKMMPGIVIADPELTVGLPPKITAATGFDAWVHCFEAFCAPGFHPLADG
                     VALEGMRLIKTYLPRACKNGGDMEARSRMLAAASMGATAFQKGLGAVHAIAHPVGSFF
                     DTHHGLTNAVITPYVIVHNRDAIAERMKVVARVLDLQKPGVDGVLAWVLDFRKELNIP
                     HSLAEIGVTLRNPEEIGREAALDPSAGGNPIPVDAATLERIFRSAVKGDLSLKS"
     gene            6135..6605
                     /locus_tag="VEW08_10310"
     CDS             6135..6605
                     /locus_tag="VEW08_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41176.1"
                     /translation="MRLMAVVSFVLLCSCATAETQTIRLAWPSAGYAGFSESIDALVG
                     PGLVDCGFFNLIAGKPASAVRRQARACVDGAIAAGQTFKYGTERLPIDSYATEVIART
                     KDGRLWKIVFDVMIDGEAAQQWNQICQSVAVDPRTLIIDATGCIENTTGRLVSQ"
BASE COUNT         1253 a         2109 c         2139 g         1153 t
ORIGIN      
        1 gcgcaactgg gacatggact ccgaaatcat caaggcgatc ggcgcctgcg agaacataga
       61 ccgcgagcac gccggcgcca tcgatctcac cgacgtgctg acagtcagtg ccgcgctgat
      121 cgcgctcggt ccggcgccgc agtcggagca ggcgcctttt cccggcatgc ccgccgcgcg
      181 ccggctggga ctggatgccg aggcttgcgc ggcggcgctg gctgagtccc gcgacgagat
      241 cgacgcattg cgacaggcgc tcaacgtatg agccaggcgg aaaccaccag cacgatcgag
      301 tcgcgcgcga tcccgaacac cgtggtgttc ggcttcgtca aggcgctggc cctggagttg
      361 tcacagggca gggtcgacct gccgtccgtg ccggacatcg ttctcaagat gcagcgcgcg
      421 ctggccgacg aaaatgtcag caacgagacc gtggtgaagg tgctgggttc cgagccggtg
      481 ctcgcgagca agctgctcaa catggcgaac tccgccgcac tcaatacctc gggccgcaag
      541 atcgccgatc tgcgcatggc cgtcgcccgc gtgggattca acatcgtgcg ctcggcggca
      601 ctgagctttg cgatggagca gctcaagagc gcaccgcagt tcaagcatct cgagccgatg
      661 ctcgacgcgc tgtggaaaca cagcgtgcac gttgcggcgc tgtcacacgt ggtcgcgagg
      721 cgcttcacct cgctcaatgg cgataccgcc ttgctcgcgg gcctgatgca caatgtcggc
      781 cgcatctaca tcctgacgcg cgccagcggc cacccgtcgc tgttcgccga tcagctcact
      841 tatcagtcca tcgtgcgcga ctggcacacc aacgtggcca aggcgctgct ggagaactgg
      901 caggtggccg atgaaatcgt cgacgcggtg agcaatcacg aggacatgaa ccgcgaggtt
      961 cgcggcccgg tgacgctcac ggacgtggtc gcgcttgcgt cccagcttca gcagcacacc
     1021 atgcagggtg cgctgatcga tcccacggaa cctctggtgt gggcgctcaa acgcctgcag
     1081 gtcaaatacg cggattgcca gagcgtcatc gacgaatccg cttcggacat cgaagccctc
     1141 aaaactgtgt taggcgggta aaaggacatg gcccagatcg cactcggaac caccaccatc
     1201 gagaatatcg tcgcgcacga tgccgcggcg ctcgagaagg acctcgcgaa tactcgagcg
     1261 gacgcttcgg tgtcactgcc cggcgtaccg cagattgtca cgcggctgca gcagctgctg
     1321 agcgatccgg acgtcagcat cccgcagatc gtgccggtca tcaattacga gcccatcctc
     1381 gtcggccgcg tgctgcagat gggcaattcc gcggcgctca atccgacccg tcgcatggtc
     1441 accgacctga agacggcagt gacccgcgtg ggcttcacgc tgttgcgcag cgcctcgctg
     1501 gcgtatgcgt tgcggcaggt gtcgcaggcg cactcggtca aggacatccg cccgcacctc
     1561 gaagcgttgt gggagcgcag ttgctggacg gcggccgtgt cctacgtgat cgcgcgcaag
     1621 ttcacgaaag tgaaccgcga tctcgcattc ctcggcggac tcatgcacgg cgtgggcaag
     1681 ctgttcatcc tcacgcgcgc cgcgcattac ccgttcgtgt tgcgcgaccg gaccaagtac
     1741 ggcgccatcc tgcgcaagtg gcatgcgtcg tttgccaagg gaatcctcac cggctggaag
     1801 gtgaacgacg atgtcatcga agccgttggc cagtacgaga acctgaatcg tgaggtcatc
     1861 ggcgatgatc cggatctcac cgacatcctg gccaccagct atctcgtcgt gggccacacc
     1921 gggcgcatgc aggatctgtc catgaccgtg gagaagatcg catcgttctc gcggctgcgc
     1981 ctcgacctca tggccgtcga agagacgctc gagcaggcca aagaagagat cgaggacctg
     2041 aggctggcta tcaagtaggt gccgggtggg gaaagtgggg agaacgggtt gagcggattt
     2101 ggcccttctc cccactttcc ccacccggca cctaccgctc atactgcggg gcactcatat
     2161 aagaagagtg cgccatgcag ccagaaaacg tccggacagc ggccgatgcc cgcaagatcg
     2221 tcgaggagcg caagctcgat cacgtcaagg tcggggtctt cgacaacgac ggcatcatgc
     2281 gcggcaagta catgggccgc gacaagttct tctcggcgct cgacaagggt ttcggcttct
     2341 gcgacgtggt gctgggctgg gactcgaacg accagctcta tgacaacgtg aagtacacgg
     2401 gctggcacac cgcctacccg gacgcacccg tccgcgtgct gcccgacacc tgccgcaatg
     2461 tcccttttga aggcgacatg gtcatgttcc tcggcgagtt ctccgagaag gccgaagcgg
     2521 tgtgtccgcg cgcaacgctg cggcgcgtcc tcaggaaggc cgacgacctt ggctacctgg
     2581 tgaacgccgc caccgaattc gaattcttca tgttcaggga aactcccgag tcgatccgcg
     2641 ccaagggctt ccgcgatctg acaaccatga cgccgggctt tttcggctac tccgtactgc
     2701 gctcgagcgt gcactcggag ttctatcacc aactcatgaa catgtgcacc gggttgcgca
     2761 ttccactcga aggcctgcac accgagaccg gcccaggcgt gctcgaagcg gccattcaat
     2821 attgtgaggc gctcgaagcc gccgaccgcg gctcgctgtt caagaccttt acgaaaattc
     2881 tcgcgcagcg aaacggcatg atggcaacgt tcatggccaa gtggtcgaag gactggccgg
     2941 ggcagagcgg gcacatccac acctcgctcg tgcgcaaggc cgatggcaaa gccgcgttcc
     3001 acgacacgac gaagccgcac aacatgtccg acgagatgcg ctggtacgtg ggcggtcagc
     3061 agaagctcat gcccgagttg ttagccatgg tcgcctgcac ggtgaacagc tactcgcgcc
     3121 tgattcccgg gttctgggcg ccgacctcgg ccacgtgggg tgtggaaaat cgcaccaccg
     3181 cgttgcgtgt gattccaggc ggtgcgtcga gccagcgcgt ggaatatcgc attgccgcgg
     3241 ccgacatcaa tccgtacctg gcgctggccg tggccatcgg ctccgggctc cacggcatcg
     3301 agaaccggat cgagcccgat ccgatggtcg aaggcaacgc ctacgacaag aaatttccga
     3361 agacgcgatc gctgccacgc acgctcaccg aagcgtccga gtggctcgag aagtccaggg
     3421 ccggccgcgc gttgttcgga gatgcgttcg tggagcacta cgcggcgagc cgcgagtggg
     3481 aagaccgcga gttccgtcgc gcgatcaccg actgggagct ggcgcgttac ttcgagatca
     3541 tctagcgaac agctggtaaa agaaaatcat gaatcacagc ggcattcaga aaaccatcag
     3601 cccggtcgac ggccgggtct acgtcgagcg tccgctcgcg acctcggcgg agatcaacga
     3661 gacgctgcgc accgcgcgcc atgcgtttac cgagtggcgc aacacgtcta ttgccgatcg
     3721 cgccgccatc ctcacgcgtt tctgcagcga gttcgaaaag cgcggctcgc agatcgccga
     3781 agagctcacc tggcagatgg gccggcccgc gcggtatgcg cccaacgaag tgcgcggcac
     3841 gctcgagcgc gcgcggcaca tgatcgccat cgcgccgcag gcgctgggcg acgtgaatac
     3901 cgaaccgaag gacaacttca ggcgcttcgt gcggcgcgag ccgctgggcg tggtgttcac
     3961 cgtcgccgcc tggaattatc cgttcctcat tgcggtgaac agcgtgattc cggcgctcat
     4021 ggccgggaac gtggtggtgc tgaaacattc cgcacagacg ccgctcgccg ccgagcgatt
     4081 cgccgaatgc ctccaggccg cggggttgcc gaagggcgcg ttccaggtcc tgcatgcctc
     4141 gcatgaggac acggaccacg tcatccgcga ccctcgcgtg gacttcgtcg cgttcacggg
     4201 ttcggttgcc ggcggccgcg ccgtgcaacg cgccgcggcg cagcgcttca tcggcgtggg
     4261 cctggagttg ggcggctgtg acccggtata tgtgcggcac gacgccaacc tggaccacgc
     4321 catcgaaaac atcgttgacg gcgcgtattt caactccggg caatcctgct gcggcctgca
     4381 gcggatctac gtgcatcact cgctctatca caagttcacg gaaggctttg tcgagctcac
     4441 gcgcaagtat tcattgggcg accccaccga cgcggccacc acgctcgggc ccctggtgcg
     4501 caccgccgcg gcagatggtg tgcgcgcgca gatccaggcg tccattgccg ccggggcgac
     4561 tggcgccatt cccgaaggtt cgtttgccag gagcgtggcc ggcacgccat acctggcgcc
     4621 gcaggtgctg ctcgacgtgg accaccacat gccggtcatg cgcgaggaga ttttcgggcc
     4681 cgtggccggc atcatgaagg tgagctcgga tgacgaggcc gtggacctca tgaacgacag
     4741 tgatttcgga ctcacggccg ccatctggac cgaagatgcc gatgcggcgc tcgccatggg
     4801 ccagcgcgtg aacaccggaa cctggttcat gaaccgctgc gactacctcg atcccgcgct
     4861 ggcgtgggtg ggcgtgaagg attcggggcg cggctgtacg ctttcggtgg tcggctacga
     4921 acatctcaca cgcccgaaat cattccatat gagaatcaag acatcatgac gattccgatg
     4981 aagggcaact ggaattatcc gacgcgaatc tggtcggggc cgggacgcat cgccgaatta
     5041 cccgccgcgt gcgccgatct cggcatcgtg aggccgctgg tcgtcaccga caatggtctg
     5101 cgcgattcgc agatgatcag gcgcgcggtg gcttcgctgc ccggcgcggg gttgtttgcc
     5161 gacgtgcgcg gaaatccggt ggccgccaac gtcgaagcgg ggctcaaggc ctatcacgcc
     5221 ggcgcgcacg atggcgtggt ggcattcggc ggaggctcgg ggctcgatgc cggaaaggtc
     5281 atcgcattca tgtcggggca gacgcgtccg ctctgggact tcgaagacgt cggcgactgg
     5341 tggacacgcg cagatccgaa aggtattgcg cctgtggttg cggtacccac cacggccggc
     5401 acgggcagtg aagtgggccg cgccggcgtc atcctcaatg aagagacgca ccagaagaag
     5461 atcatttttc atccgaagat gatgccggga atcgtcattg cggatcccga gctcaccgtg
     5521 ggcctgccgc cgaagatcac cgcggccacg ggtttcgacg cgtgggtgca ttgcttcgaa
     5581 gcgttctgcg cgccggggtt ccacccgctg gccgacggcg tggcgctcga gggcatgcgt
     5641 ctcatcaaga cctatctacc acgcgcctgc aagaatggcg gcgacatgga agcgcgctcg
     5701 cgcatgctgg ccgcggccag catgggcgcc acggcgttcc agaaggggct cggtgccgtc
     5761 catgccatcg cgcatccggt cgggtccttt ttcgacacgc atcacgggct caccaacgcc
     5821 gtgatcacgc cgtatgtcat cgtgcacaac cgcgatgcaa tcgccgagcg catgaaggtg
     5881 gtggcgcgcg tgctcgacct gcagaagccc ggcgtcgacg gtgtgctggc gtgggtgctc
     5941 gactttcgca aggagctcaa cattccgcat tcgctggcag agatcggcgt gacgctgcgc
     6001 aacccggaag agatcgggcg cgaagcggcg ctcgatccat cggcgggcgg caatcccatt
     6061 ccggtcgacg ccgcgacgct cgagcgcatc ttccgctcgg cggtgaaggg cgacctctct
     6121 ctcaagagct gaccatgcga ctcatggcgg tggtgtcgtt cgtgctgctc tgctcgtgcg
     6181 ctactgccga gacgcaaacc atcagactgg cgtggccttc ggccggttat gcaggctttt
     6241 ccgaatccat agacgctctc gtaggtccag ggctcgtcga ctgcgggttt ttcaatctca
     6301 tcgcgggcaa gccggcatcc gctgttcgaa gacaggcgcg ggcatgcgtg gacggggcga
     6361 ttgcggccgg acagactttc aaatacggaa cggaacgctt gccgatagac tcctacgcga
     6421 ccgaagtcat cgcgcggacc aaagatggca ggctctggaa gatcgtcttc gacgtcatga
     6481 tcgatggcga ggctgcgcag cagtggaatc agatctgcca gtccgtggct gtcgatccgc
     6541 gaaccttgat catcgacgcg accggatgta tcgagaacac tacgggccgg ctggtgagcc
     6601 agtgacccga atcctcgtca tcgacggcaa cgagaaggcg acgcgtgaca aaca
//
LOCUS       DASQJS010000255         3332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2348205, whole genome shotgun sequence.
ACCESSION   DASQJS010000255 DASQJS010000000
VERSION     DASQJS010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2348205"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="VEW08_10315"
     CDS             <1..1144
                     /locus_tag="VEW08_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HYJ41177.1"
                     /translation="FDYFEGQGKYNRVIQARRQPGSGFKPFMYAAAMAGEFTPASMIL
                     DAPIIMDDPNLEEVWRPENSGGGFRGPMRLREALVQSRNLVSIRLLQGIGIRPVIDYV
                     QNFGFRKDQLPGNLTLALGSMGATPLEVATGFTVFANGGFKVEPYYIDRIEGPGGQIV
                     YAAEPRTVCAECVQPVQTISVAERAKNAEVSATIVPPTPLAPAARRTQPAERVISPQV
                     SFLMNDIMKDVITRGTGRRALALGRADLRGKTGTTNLAVDTWFNGFNDSLVASVWVGY
                     DDNRPLGDLEEGARTAVPIWVDYMREALRGVPEKPRTIPEGIVEMKINARTGGTRDAD
                     VDPVFEYFRVDKLPSEEGYVGDPGTGPLDIDPTSPDSSPSNPDPIF"
     gene            1147..1773
                     /locus_tag="VEW08_10320"
     CDS             1147..1773
                     /locus_tag="VEW08_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41178.1"
                     /translation="MKKLPPRAENLRRAIAQEAARIMSEHGIRDFLTAKRKAAERFGV
                     TDGSVLPKNREIEDALAEYHRLFGGTKHGEVLAGQRNAALSAMQMLVDFEPRLVGSVL
                     AGTATEHNDIQLHLFAERPESITFKLMDLGIDHEVVERRVRYSADRVVAYPGVHFEFD
                     DHVVDATVFPIDGIRQAPVSPVDGKPMRRIDADELEVLLDAEDAPTAS"
     gene            complement(1810..3105)
                     /locus_tag="VEW08_10325"
     CDS             complement(1810..3105)
                     /locus_tag="VEW08_10325"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HYJ41179.1"
                     /translation="MADKTLELIDTATGKKAVLPVRSGTVGPSVVDIAPMVKEFGAFT
                     YDPGYGATAAVESKITYIDGEAGVLTHRGYPIEQLAAKSSFIEVCYLLLNGELPGKTQ
                     LEQFEHSIRNHTMINESLLRFFGGFHHNAHPMAMVSAVVASMSAFYHDSMDIHNPRHR
                     EIFAHRIVAKLPTIAAAAYKHSLGQPFVYPRNDLSYCANMLHMFFAVPCEPYKVDPVA
                     AEALDLLFILHADHEQNASTSTVRLAGSTGANPYAAISAGVSALWGPAHGGANEAVLE
                     MLEEIGTADQIPKYLAMAKDKSSHFRLMGFGHRVYKNFDPRAKIIRDMCHKVLKKLGK
                     QDNPLLELAQHLEEAALKDEYFVARKLYPNVDFYSGIIYSALGIPRSMFTVMFAIART
                     VGWVAHWQEMISDPAMRIGRPRQLYTGPTKRDYQDIGTR"
BASE COUNT          632 a          996 c         1066 g          638 t
ORIGIN      
        1 cttcgactac ttcgaaggcc agggcaagta caaccgcgtg atacaggcga gacgccagcc
       61 tggctcggga ttcaaaccgt tcatgtatgc ggcggcgatg gcgggcgagt tcacgcccgc
      121 gtcgatgatt ctcgatgcac caatcatcat ggacgatccc aatctcgaag aagtatggcg
      181 gccggaaaat tccggcggcg gatttcgcgg acccatgcgc ctgcgtgaag cgctggtgca
      241 gtcgcgcaac ctggtgtcca ttcggctgct ccagggaatt ggcattcgtc cggtcatcga
      301 ctacgtgcag aacttcggat tcaggaagga ccagttgcct ggcaacctga cgctggcgtt
      361 gggcagcatg ggtgcgacgc cgctggaggt tgccaccgga ttcacggtgt ttgccaacgg
      421 tggcttcaag gtcgagccct actacatcga ccgcatcgaa ggtcccggtg ggcagatcgt
      481 ctacgccgcc gagccgcgca cggtgtgcgc ggaatgcgta cagccggttc agaccatctc
      541 tgtggcggag cgtgcgaaga atgccgaagt cagcgcaacc atcgtgccgc cgaccccgct
      601 cgctccggca gctcgccgca cgcagcccgc ggagcgcgta atttcaccgc aagtgtcctt
      661 cctgatgaac gacatcatga aggacgtgat cacgcgcggc accggccggc gggcgcttgc
      721 gctcggtcgc gccgatttgc gtggcaagac gggcaccacc aaccttgccg tcgatacctg
      781 gttcaatggc ttcaatgaca gcctggtcgc cagcgtctgg gtgggctacg acgacaaccg
      841 gccgctgggc gacctcgaag aaggcgcgcg cacggcggta cctatctggg tggactacat
      901 gcgtgaagcg ctgcgcggcg tacccgagaa gccacgcacg atccccgaag gaatcgtcga
      961 gatgaagatc aatgcacgca ccggcggcac gcgggatgcg gacgtcgacc cggtgttcga
     1021 atattttcgc gttgacaaac taccctcgga agaaggttat gtcggtgatc ccggcacagg
     1081 cccgttggac atcgatccca cttccccgga ttcatcgccg agcaaccccg acccgatctt
     1141 ctgaccatga agaaattgcc tccccgtgcc gagaacctgc ggcgcgcgat cgcgcaggaa
     1201 gccgcgcgca tcatgtcgga gcacggtatc cgtgatttcc tcacggccaa acgcaaggcc
     1261 gccgagcgct tcggcgttac cgacggctca gtgctgccaa agaatcgcga gatcgaggat
     1321 gcgctggccg agtatcaccg gctgttcggc ggcacgaagc acggtgaggt tctggcgggg
     1381 cagcgtaatg ccgccctgtc ggccatgcag atgctcgtgg atttcgagcc gcggctcgtc
     1441 ggctccgtgc tcgccggcac ggccaccgaa cacaacgaca ttcagttgca tctgttcgcc
     1501 gaacgccccg aatccatcac gttcaagctc atggatctcg gcatcgatca cgaggtggtc
     1561 gagcggcggg tgcgatacag cgcggatcgg gtagtcgcct accccggcgt gcatttcgaa
     1621 ttcgacgacc acgtggtgga tgccaccgtg tttccgatcg atggcatccg gcaggcgccg
     1681 gtcagcccgg tggatggcaa accgatgcgc cgcatcgacg ccgacgagct ggaagtattg
     1741 ttggacgccg aggacgcgcc aaccgcgtcg taaccgcggc ccgcgttgag tgttatgcgc
     1801 gcagcgcctc tagcgcgtgc cgatatcctg atagtcgcgc ttcgtcggcc cggtatagag
     1861 ctggcgcgga cgcccgatgc gcatggccgg atcggagatc atttcctgcc agtgcgccac
     1921 ccagccgacg gtacgcgcga tcgcgaacat caccgtgaac atcgagcgcg gaattcccag
     1981 cgctgagtag atgatgccgg aatagaagtc gacgttcggg tacagcttgc gcgcgacgaa
     2041 gtattcgtcc ttcagcgcgg cctcttccag gtgctgggcg agctccagca gcgggttgtc
     2101 ctgcttgcct aacttcttca gcaccttgtg gcacatgtcg cgaatgatct tggcgcgcgg
     2161 atcgaaattc ttgtagacgc gatgaccgaa acccatgagg cggaagtggc tgctcttgtc
     2221 cttggccatc gcgaggtatt tcggaatctg gtcggcggtg ccgatttcct cgagcatctc
     2281 gagcacagcc tcattggcgc cgccatgggc cgggccccag agcgcggata ctcccgccga
     2341 gatcgcggcg tacggattcg cgccggtcga gcctgcgagg cgcacggtcg aggtgctcgc
     2401 attctgctcg tgatcggcat gaagaatgaa caggaggtcc agcgcctcag ccgcaaccgg
     2461 atcgaccttg tatggttcgc agggcaccgc gaagaacatg tgcagcatgt tcgcgcagta
     2521 ggaaaggtcg ttgcgcggat agacgaacgg ctgtccgagc gaatgcttgt aggccgcggc
     2581 tgcgatggtg ggcagcttgg cgacgatgcg gtgcgcgaag atttcgcggt gccgcgggtt
     2641 atggatgtcc atcgagtcgt gatagaacgc ggacatcgac gctaccaccg ccgacaccat
     2701 ggccatcggg tgcgcgttgt ggtggaaacc accaaagaac cgcagcaacg actcgttgat
     2761 catcgtgtgg ttgcggatgc tgtgctcgaa ttgctcgagc tgagtcttgc cgggcagttc
     2821 gccgttcagc agtagatagc agacctcgat gaacgagctc ttcgcggcga gctgctcgat
     2881 ggggtaaccg cgatgcgtca gcacgccggc ttcgccgtcg atgtaggtga tcttgctctc
     2941 gaccgcggcg gttgcaccat aaccgggatc gtaggtaaag gcgccgaatt ccttgaccat
     3001 tggcgcgatg tcgacgaccg acggaccgac cgtgccggag cgaacgggaa gcacggcctt
     3061 cttgccggta gcggtatcta tgagttcgag tgttttgtca gccatgattc gcaagctcta
     3121 acgacgcgac gagccttaca cggtccgcga agggaatcaa ggcaacgagc gcgcgcgagc
     3181 gcgcgtactt tgaatcgtta ctcgacgatc ttgaaaagct ggccggcttt cggccgcttg
     3241 ttcggataca tcgcgttgat gagttcgaga gtttccttct tgaatttttc ttcgggcatg
     3301 tctgccgcgt agtcggaaag tttggtcccg tc
//
LOCUS       DASQJS010000256         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_457946, whole genome shotgun sequence.
ACCESSION   DASQJS010000256 DASQJS010000000
VERSION     DASQJS010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457946"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VEW08_10330"
     CDS             <1..366
                     /locus_tag="VEW08_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ41180.1"
                     /translation="FYRILANGIRDMQRRRTVRGRIMAWLPVRDADDDEEFDPIAQAP
                     SQDAGPAKRLELDEAIGVLELAVAELPPRQQQAFLLRNFEGLDVNETASAMGCSEGSV
                     KTHYFRALESLRAKLGEVF"
     gene            376..720
                     /locus_tag="VEW08_10335"
     CDS             376..720
                     /locus_tag="VEW08_10335"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HYJ41181.1"
                     /translation="MTDPTDFEKRTQEVLDESAGRLDGHTRSRLTQARHAALAQLEQP
                     ARYWWRSYVPAGAAAAVTVLAVVMWSNPADRPSASPVEDMELLADSDAPDFVDDGDDL
                     EFYEWAAGEVET"
     gene            717..908
                     /locus_tag="VEW08_10340"
     CDS             717..908
                     /locus_tag="VEW08_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41182.1"
                     /translation="MSDPKPAEPDDDLLEFLGGIDEANDEFQDEDFSDFLAKTDIDKL
                     AAQKARAPRKDVKQDDKSE"
     gene            901..1437
                     /locus_tag="VEW08_10345"
     CDS             901..1437
                     /locus_tag="VEW08_10345"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HYJ41183.1"
                     /translation="MSNVRHTRRAALAAMFAVGMHLATAGRARAELPPVENADAPATA
                     WSSLSPDEQKALSRYSEKWSSMPAEQQQRLLRGTRRWLAMTPDQREKAQKRYARWKEL
                     SPEQKELARKRWHKYRELPPDQQARVRENYHRFKKLTPEQRQRLRERWQNATPEERQR
                     MLERRRQRQQQRPPPPPR"
     gene            complement(1438..1917)
                     /locus_tag="VEW08_10350"
     CDS             complement(1438..1917)
                     /locus_tag="VEW08_10350"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ41184.1"
                     /translation="MITKRDLATAVIAASVTLACVSMAEEPGKVLDSTAWLWTDLPAK
                     KTDVGELRAVVRQPTRTLDELEMHITTLNPHQASHPPHTHPNEEMVIVKEGTLQAHVN
                     GKEVVVPAGGVLFYASMQPHAVQNIGDTPATYYVINWASPGSKTKKIPPKPAPSTAQ"
     gene            2006..2524
                     /locus_tag="VEW08_10355"
     CDS             2006..2524
                     /locus_tag="VEW08_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HYJ41185.1"
                     /translation="MGSKLEQITEKALFASRWILAPIFLGLSLALLALGIKFFQEAFH
                     LVYDVLTTNKDESQLVLVALALIDISLVGSLIVMVMFSGYENFVSTIDAKGTDTLGWL
                     GKMDSGTLKLKVAASIVAISSIHLLRVFMKAETTDNDKIMWYVILHMTFVFSAVLLGV
                     LDKMSFAKHRDH"
BASE COUNT          498 a          826 c          878 g          472 t
ORIGIN      
        1 ttctatcgca tcctcgcgaa cggcattcgc gacatgcagc gccgccggac ggtacgcggc
       61 cggatcatgg cttggctccc ggtgcgcgat gcggacgacg acgaggagtt cgatccgatt
      121 gcgcaggcac ccagccaaga cgccgggccc gcgaagcggc tcgaactcga cgaggccatt
      181 ggcgtactgg agttggccgt ggccgaattg ccgccgcgcc agcagcaggc gttcctgctg
      241 cgtaatttcg aaggactcga cgtgaacgaa acggcgagcg ccatgggctg ctccgaaggc
      301 agcgtgaaga cgcattactt tcgcgcgctg gagagcctgc gcgcgaagct gggcgaagtg
      361 ttctaggggc aaggtatgac ggatccgacg gatttcgaaa aacgtacgca ggaagttctc
      421 gacgaaagcg cagggcgcct cgatggacac acgcgttcgc ggctgaccca ggcgcgccac
      481 gctgcattgg cgcagctcga acagccggcg cgttactggt ggcgttccta tgtgccggcc
      541 ggcgcggcgg ccgcggtcac cgtgctcgcc gtggtcatgt ggtcgaaccc tgcggaccgg
      601 ccgtcggcca gccccgtcga agacatggag ctgctcgccg attcggacgc gccggatttc
      661 gtggatgacg gtgacgatct cgagttctac gaatgggctg caggcgaggt ggagacgtga
      721 gcgatccgaa gccggccgaa cccgatgacg acctgctcga gttcctcggt ggcatagacg
      781 aggccaacga cgaatttcag gatgaagact tttccgattt tctcgcgaaa accgacatcg
      841 acaagctcgc ggcgcagaag gccagggcac cccggaagga cgtcaaacag gacgataaga
      901 gtgagtaacg ttcgccacac ccgacgagcc gcgctggccg cgatgttcgc ggtcggcatg
      961 cacctggcca cggctggcag ggcgcgcgcc gaactgcctc ccgtggagaa tgccgacgcg
     1021 ccagcgacgg cgtggtcgtc gttgtcgcca gacgagcaga aggcgctcag ccgctacagc
     1081 gagaagtgga gttcgatgcc cgccgaacag cagcagcggc tgttgcgcgg tacgcggcgc
     1141 tggctggcga tgacgcccga ccagcgcgag aaggcgcaga agagatacgc gcgctggaaa
     1201 gagctgtcgc ccgagcaaaa ggaattggcc cgcaagcgct ggcacaaata ccgcgagctt
     1261 ccccccgacc agcaggcgcg ggtgcgcgaa aactatcacc ggttcaagaa actcacgccc
     1321 gaacagcgcc agcggctacg cgagcgctgg cagaacgcca cgccggaaga gcgtcaacgc
     1381 atgctggaac gtcgccggca gcggcagcaa cagcggccgc cgccgcctcc ccgctaacta
     1441 ctgggctgtc gaaggcgcgg gcttcggcgg aattttcttc gtcttgctgc cggggctggc
     1501 ccagttgatc acgtagtagg tggcgggcgt gtcgccgatg ttctgcaccg cgtgcggctg
     1561 catcgacgcg tagaacagca ccccaccggc cggaaccacg acctccttgc cgttgacgtg
     1621 cgcctgcagg gtgccttcct tgacgatgac catctcttcg ttgggatgcg tgtgcggcgg
     1681 gtgcgaggcc tgatgcgggt tcaacgtcgt gatgtgcatc tcgagctcgt cgagcgtgcg
     1741 cgtgggctgg cgcaccacgg ctctcagttc accgacgtcg gtcttcttcg ctggtaggtc
     1801 ggtccagagc cacgcggtgg agtccagcac cttgcccggc tcctcggcca tcgatacgca
     1861 ggccagggtg acgctggcgg cgatgacagc cgtggcgagg tctcgtttgg tgatcatggg
     1921 caaacctccg cgatgggggc ggcagactac caagcgcctt tctatggcta caatacgcgc
     1981 ggcctcatac cttcggagta gacaagtggg ttccaagctc gaacagatca ccgagaaagc
     2041 gttgttcgcg tcgcgctgga tacttgcgcc gatcttcctc gggttgtcgc tcgcactgct
     2101 ggcgcttggc atcaagttct tccaggaggc ctttcacctc gtctatgacg tgctgacgac
     2161 gaacaaagac gagtcgcagc tcgtgctcgt cgcgttggcg ctgatcgaca tctcgctggt
     2221 tggcagcctc atcgtcatgg tgatgttttc gggctacgag aattttgtgt ccaccatcga
     2281 tgcgaagggc actgacacgc tcggctggct ggggaagatg gattccggca ccttgaagct
     2341 caaggtcgcc gccagcatcg tggccatttc atcgatccac ctactgcgcg tcttcatgaa
     2401 ggcagagacc accgacaacg acaagatcat gtggtacgtg atcctgcaca tgacgttcgt
     2461 gttctccgca gtgttgctcg gcgtgctcga caagatgtcg ttcgccaagc accgcgacca
     2521 ttaggtgccg ggcgaggaaa gtggggagtg cgtggggtga gccatcgggg agtcgcacag
     2581 atcgcgcgca ctccccactt tcctcacccg gcacctacca tcaacccgcg gccttgatgc
     2641 cgttgacgag ctcgcccacg gccttctgtc cgtc
//
LOCUS       DASQJS010000257        15872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1090847, whole genome shotgun sequence.
ACCESSION   DASQJS010000257 DASQJS010000000
VERSION     DASQJS010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15872
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090847"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="VEW08_10360"
     CDS             <1..1548
                     /locus_tag="VEW08_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ41186.1"
                     /translation="HLLSNILMRQQRFDEAIALLNAWRGRKDQSDWLAYAQYNLGVAL
                     VRKGLVTEADPFLTAVGTIESGRRELRSLRDRSNLALGYAYLQNNQPALAKPVLERVR
                     LDGPFSNKALLGVGWADAAEGQFQQALTPWMELRDRNLLDSAVQEAYLAIPYAFAKLN
                     ANAQAAENYELAIRSFDTETVNLDASIAEIDDGTMLDKLLQRDANNRVGWFWQLKEVP
                     DAAQSRYLYHLLAGHDFQEGLKNYRDLAFMGGTLSRWQDSIVAFDDMLATRERAYAER
                     VPQADAVLNSGRLDQYGAERATTGGQLDQIEAAQDVAALGTAEERAQWARIVRLEAAL
                     LAAPNDEETNVIREKTRLAKGVLYWRLAESFKARVWNERRTLKDLDQALREAQNRWVR
                     VQKARGSMPDYTGQFAERVEALRLRLEAAQQRLAGVAEQQNGLLESLARTELEQQKER
                     IGTYQIQARFALASIYDRAAIGGGSVATPSEGATESQGDLEVVPPPGPSDGSPPGETP
                     PPVEPPK"
     gene            1545..5066
                     /locus_tag="VEW08_10365"
     CDS             1545..5066
                     /locus_tag="VEW08_10365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ41187.1"
                     /translation="MTFAGKQIILVAALCAGIAINASAAAPKKKEKTIGELSSRPVVV
                     QPDQKVEASAARAMDNYRRFLELQKTDPQLRAEALRRLGDLNMEAGEGERLAGEASTI
                     DMQGAEAIKLYSSLLKSYPDYPRNDQVLYQLARAYETTGQPEKALSTLNEILRKYPKA
                     PQLDEVQFRRGELLFSARDYRSAESAYQYVVNKGDKAAFLSQSLYKQGWSLFKLGLNN
                     ESLQPFAGVLDRTMLQKDSRALIPLDKLPRANRELADDTLRVMAVTFSYDDDSVGAID
                     TFLSAHGNPAYAPIVYSRLGDLYVEKERFQDAAAVYRAYATREPNSEFSPGLSMQAID
                     AYRKGGFTELVLEGKREYVALYNYGTPFWSGRNKADYPNIAKELKTNLKDVATYFHAE
                     AQKTKKVDQFREAARWYRTYLESFPDDPESFGTNYLLSEALYSSEDYQGAATEFARTA
                     YNYPRNPRSAAAAYAALGSYAKYEEKLPAAEKAESHKLAVDAGVKFGTSFPEHPDSAG
                     VLTRAAEDIFATQDLQRSIEVANLVLAHQPPADQPKRRIAYTIIGQANFDLLQFSEAE
                     KGYIAARDLLPPNDKMRADLTERIASAVYRQGEAKQKAGDNLGAVDDFLRISQVAGTS
                     KIAAQAEYDAGAQLIVLKEWPRAIQVLEHFRANNPKSEFSADVTRKLAVAFGETGQAG
                     QAAVEFERIAMNPAESKDIQREANLQAAELYTKAGNTPKAVGMLERFVVTYPTPVSDS
                     IEARWKLAEISGTAGNLERQRTWQREIVNADRTAGAGRTDRTQYLAAKSQLALASPAR
                     DEYRSIALVLPLKTSLAKKRKAMEAALGGYKAAADYRVAEVTTVATYEIAEIYRQLGK
                     DIMKSERPKKLAAEQLDEYNSLLEEQAFPFEEQAISTHEINTKRVRDNVYDDGVKKSF
                     AALAELKPGRYGKSEVMSASFDALVPPLPPAPALPDPPITTSADPNAKPVSPATAAPA
                     VPAFNVPKPPARANTEFTRALTLMRGSDATQAVLEMQVLTQSYPDLPGPYANLGVLHR
                     NANQLAESEAALAKATERAPWDAQTWTEYGITLRQAGKFAEARAAYEHAIKANPSYAP
                     AHRNLGVLLDLFLEDPLTAQSEFETYKTLTGEDKPVSGWLAELRARNMVRAHASEAAP
                     ESASEPAPESAPPPENDAAPKPQGGAT"
     gene            5063..5551
                     /locus_tag="VEW08_10370"
     CDS             5063..5551
                     /locus_tag="VEW08_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41188.1"
                     /translation="MNKLLFVALLLLAGAALAQTPAPAPATPPQDAMEPGVNTSAPAA
                     KAPDAMEPGVNTSVPAAAPAKPAATTNKPVARPGAAKSNAKDRLDLESTQITGNRELP
                     KVLYIVPWRSAEMGDLVGRPVNSLLDEVLEPVDRDVFRRQNRYFEALQPNEAAPASNP
                     GK"
     gene            5643..6341
                     /locus_tag="VEW08_10375"
     CDS             5643..6341
                     /locus_tag="VEW08_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYJ41189.1"
                     /translation="MGFINTVIVFFKEGGFFLYPLLIIFVVGVAIAIERWLYLTVETS
                     RNRSLWEDVAPHLGAGNFKQAITLANNSQAQIGTVLKYGLARLSTARRRDDIQQAMEE
                     SLLEIVPRLEKRTHYLSALANIGLLVGLLGTILALIHAFGAVSQANPAEKASLLAAAI
                     SEAMNNTASGLFVAIVLLLAHMFLESKTTSLIDSLEIAVVKFLNSITERYSEPAAPAP
                     QGGMAPRAPAPRPA"
     gene            6386..6931
                     /locus_tag="VEW08_10380"
     CDS             6386..6931
                     /locus_tag="VEW08_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYJ41190.1"
                     /translation="MASSKLAARSIRNHAKYKGRNDLNIVPMIDMMVILVFFLLFTAV
                     FSNTNILELNLPAPNSSVPELPQGLQLEVIVRKNSIEIADRNSGVLRSLPKKEGEYDY
                     AGLSEFLQFVKAKYPTVLAANVLLEQDIPYDVLVQTMDNTRLWLNAKGIGLVKVELFP
                     DISVGDAPLVGAAAASVRGGK"
     gene            6928..7434
                     /locus_tag="VEW08_10385"
     CDS             6928..7434
                     /locus_tag="VEW08_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYJ41191.1"
                     /translation="MTPYRIKKHRQRGHHVSHMALVPFIDMLMMLVVFLLLHSSDVEI
                     LPNTKQITIPQSVAELKPRETVVVMLTTDALLVNGRQIATMAELRGDGKQLIIEPLRL
                     ALKEQSDSSLRAAAQGDLAEREVTIMGDKGTPFAVLKRVMATATAADYGKVSLAVIQR
                     EVEAGAGA"
     gene            7431..8339
                     /locus_tag="VEW08_10390"
     CDS             7431..8339
                     /locus_tag="VEW08_10390"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HYJ41192.1"
                     /translation="MSMGLAPYFRRFELPWDLGEETERRFLKLVRILLVISTVLAIIL
                     FLLPMPNRERVKPSDLPERVVQLIVEKPKPPPPPPPPEPEKKPEIEKPKPTEQKPVEK
                     PKPNVRNQGMLALQDELEALRDQNLDLADPQMKAPVADARSERNLITSQAGKASGGIN
                     TANMARGFGGGAGAIGTHSTTQVSHGAGLDPNAGGRVQRSGNSGKASRAREEVEIVFD
                     RNKGALYALYGRALRDQPELAGKLVLEFTIAPSGEITMCRVVSSELKDPELEKKIVAR
                     VRLIRFKPADVEPLTVSKPIDFFPAA"
     gene            8358..8717
                     /locus_tag="VEW08_10395"
     CDS             8358..8717
                     /locus_tag="VEW08_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41193.1"
                     /translation="MNLQSEIAYYTEIRIADKARLMASFLAELTAEARATYGATPDSV
                     HDAARLRFTNEMCNRISKLIEQYLGDDKTRPADDVVLRMLLSTRADKVAEKMIHAAYR
                     RALQNFERYDTTVFMNS"
     gene            9152..11824
                     /locus_tag="VEW08_10400"
     CDS             9152..11824
                     /locus_tag="VEW08_10400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ41194.1"
                     /translation="MNIRRSTLIGTAIAFALYNQTAAAQTATTDAGEELDEVIVSGIR
                     ASLEQSLEVKRNADARLEVVTAEDVGKLPAHNVADALRGLPGVNISSSSADEGGFDES
                     DRVSLRGTSPSLTQTLINGHSVGSADWFVLSQGNNVGRSVSYSLLPSELVNSVEVNKS
                     SHARLQEGGTTGTVNILTRKPFQFDKSLTVEASLGSVYSDQADSYDPQLSGLINFKNE
                     SETFGVLLQVFKQERSLRRDAQEIPGGFFTIADTDPAGISNPDLVGVQVPGLIGSTLF
                     EMERNRSGGLLELQFKPSDNVTLGLSGFLSDMDANNYNRNFMLWGGNFARAQAPNPGY
                     TVENGVLTSATYAGVAGRDYAVYDMISREATAKSNYVTFDADWRINDSLTSRFQAGTT
                     EGEGSTARQFIAEVTTNRGGGASWTSHGTSRPLDWTVGGDSNPTGVTGFGTWGNQQVT
                     ALDSEDWFNLDFSKDLDTGALTSIDFGARFADHERETQSPEGASPGDIWSDLQSGDTN
                     LYPSDFAADIDGSFPRGLWFFTPNALREAVLANSTWLSDNDGPTGRHNYGGEWKVSET
                     NLAAYVQANFAGEAWSGNFGLRYVDIDQEINSYQAVSATSDVIDVSSLFGNWIYQDTN
                     NKHSKVLPSANLKFNMGEDVVFRFAASQTMTLPDYSALGAASWGSDLNRTGGGGNPNL
                     QPVTSTNFDGNLEWYFMPRGLVSVGAFAMDLDDYIAFGTETRMLYSELTNQLEAYNVA
                     VPVNSNGSVTGLELVYEQPIGESFGFNANYTYADGKTAHTWADGSNNLVGTSKDTYNV
                     GLYFENEMFSARASYSYRTAFLIGLSGANPYYQDDFGTVSVALNYKPTDWINVSFDAL
                     NLNGPTLTYYQSATAPTAFYNNGKQYYLNLRFKF"
     gene            complement(11918..13402)
                     /locus_tag="VEW08_10405"
     CDS             complement(11918..13402)
                     /locus_tag="VEW08_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HYJ41195.1"
                     /translation="MKIDRRKLLVASASAGILASADRPASAQNRRAAAPGKFSVDGRQ
                     VTVYTTADKSDHRLSVTGTLDFKHFGQPLETQVCVFVDPSKTYQATIGFGGAITDSSA
                     ETFARIPEGKQREFLDAYFHREKGIGYTFVRTNIHSCDFSSGSYTYVAEGDKELKTFS
                     VAPDRRYRIPLIKRAAETAGGKLTFLVSPWSPPAFMKDNGSMLKGGKLKPDFYQAWAN
                     YYARFIKAYQAEGIPVWGLTVQNEPMATQRWESCIYSAEDERDFLKNYLGPTLRKEGL
                     GDKKIIVWDHNRDLIYQRVSTILADPDAARYAWGIGFHWYETWSGGEPMHDNVKLTHD
                     AYPDKPLIFTEGCVEAFNFDKLGDWKLGERYARQMINDFNNGMAGWCDWNILLDEKGG
                     PNHVANYCYAPMHADTRSGELIYTNSYYYIGHFSKFVRPGAKRIASSPSRSALLSTAF
                     VNGDGKVAVVVMNGGEKEVEYFLWVDGQAAAVKSPPRSIQTLVF"
     gene            complement(13428..14564)
                     /gene="nhaA"
                     /locus_tag="VEW08_10410"
     CDS             complement(13428..14564)
                     /gene="nhaA"
                     /locus_tag="VEW08_10410"
                     /inference="COORDINATES: protein motif:HMM:NF007111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HYJ41196.1"
                     /translation="MQLHQTFRRFVQSEQFGGVLLVGCTIVSLVLANSPLAAPWLGLW
                     HLKLGPLSVEHWINDALMAIFFLMIGLELERGIYIGALSKLRNALLPAFAAVGGMVAP
                     ALIHFAFNGGTATESGFGIPMATDIAFALAVIALVGPRVPVALKLFIVAFAIIDDLGA
                     IVLIATVYTADLSFTWLAMALGTWAALIILNCPLRVMPLWPYLIGGVFLWLCVYKAGV
                     HASIAGVLLAFAIPFSPRTSEAESPSHRLERTLHKPVAYLILPLFALANTGVPINQES
                     IAELSHVNSIGIALGLIVGKPLGVIVMCVLAVTTGVCSLPANVRWPHMVGAGLLGGIG
                     FTMSIFITNLAFVDHAAIVNSSKLAVLAASLIAGLLGLLWLKKT"
     gene            14588..>15872
                     /locus_tag="VEW08_10415"
     CDS             14588..>15872
                     /locus_tag="VEW08_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HYJ41197.1"
                     /translation="MTESPLSLILVLLAASVFVVTLARRLGLPSILGYVTVGLVLGPH
                     ALGLFPESDTAHLLAELGVVFLLFTLGLEFSWPRMVALRREVFGLGSLQVFGTAGVVA
                     VIAHLFGLQWAQSIVIGGVVAMSSTVLIVQLLTERAELNRTHGRLAFSVVLFQDIAVV
                     PFLALAAALAPGSEEFSYAGALSLVAAGTLAVCVVLAAGRWLLKPLLYVIANSRLREL
                     FTLAVLLVALASAWASQMAGLSMALGAFLAGMMLAETEYSHQVESVIRPFRDILLGLF
                     FISVGMLLDFNALWRELWLILALVVAIVVVKAVVASFATRLFVDSNFKALRTGVTLAV
                     GGEFGIALLTLLLQNQAIDPAIAQPLLVTVVLGMVLAPVILANNKRVARLLLGERGPP
                     KTAVEREDAATQAVAKREHVILCGFGRVGQNVARVL"
BASE COUNT         3121 a         5124 c         4838 g         2789 t
ORIGIN      
        1 cacctgctca gcaacatcct catgcgtcag caacgcttcg acgaagcgat cgcgctgttg
       61 aacgcgtggc gcggccgcaa ggatcagtcc gactggctcg cgtacgcgca gtacaacctg
      121 ggcgtcgcgc tcgtgcgcaa gggactcgtc accgaggctg atccgttcct gacggcggtc
      181 ggcacgatcg aatccggacg gcgcgagctg cgcagtctcc gcgaccgctc gaacctcgcg
      241 ctgggctacg cctacctgca gaacaaccag ccggcgctgg ccaagcccgt gctcgaacgc
      301 gtgcgcctcg acggcccgtt ctcgaacaaa gccttgttag gcgtgggctg ggccgacgcg
      361 gccgaaggcc agttccagca agcgctcacg ccgtggatgg aactgcgcga ccgcaacctg
      421 ctcgactccg ccgtgcagga agcctacctc gccattccct acgcgttcgc caagctcaac
      481 gccaacgcgc aggccgccga aaactacgag ctggccatcc gatccttcga caccgagacc
      541 gtgaatctcg atgcgtccat tgccgagatc gacgacggca cgatgctcga caaactcctg
      601 cagcgcgacg ccaacaatcg cgtcggctgg ttctggcaac tcaaggaagt gccggatgcg
      661 gcgcagtcgc gttacctcta ccacctgctg gctggacacg atttccagga aggcctgaag
      721 aactaccgcg atctcgcatt catgggcggc acgctctccc ggtggcagga cagcatcgtt
      781 gcttttgacg acatgctggc cacacgcgag cgcgcgtacg ctgaacgcgt accgcaggcg
      841 gatgcggtgt tgaattcggg ccgtctcgac caatacggcg cggaacgtgc cacgacgggt
      901 ggtcagctcg atcagatcga agcggcgcag gacgtcgcgg cgctcggcac ggccgaagag
      961 cgcgcccagt gggcgcgcat cgttcgcctc gaagccgcgc tgctcgccgc gccgaatgac
     1021 gaagaaacga atgtgatccg cgagaagacc cggctcgcca agggcgtgtt gtactggcga
     1081 ctcgccgaat ccttcaaggc gcgagtgtgg aacgaacgcc gcacgctgaa ggacctcgat
     1141 caggctttgc gtgaagcgca gaaccgatgg gttcgcgtgc agaaggcgcg cggctccatg
     1201 cccgactaca ccgggcagtt cgccgagcgc gtcgaagctt tgcgcctgcg gctggaagcc
     1261 gcgcagcagc gtctcgccgg cgtcgccgag cagcagaacg gcctgctcga atcgctcgcg
     1321 cgcaccgagc tcgagcagca gaaagagcgc atcggcacct accagatcca ggcgcgtttc
     1381 gcgctggcgt ccatctacga tcgcgccgcc atcggcggcg gttcggtggc cacgccgagc
     1441 gagggcgcaa cggaatccca gggcgatctc gaagtggtgc cacccccagg tccttcggac
     1501 gggtcgccgc cgggcgagac gccaccaccg gtggagccgc cgaaatgacg ttcgccggca
     1561 agcagattat tttggttgcg gcgctgtgcg ccgggatagc gatcaacgcc tcggccgccg
     1621 cgccaaagaa aaaagagaag acgatcggtg aattgagttc gcgcccggtc gtcgtccagc
     1681 ccgatcagaa agtggaagcc agcgccgcgc gcgccatgga caactaccgg cgtttcctcg
     1741 aactgcagaa gaccgatccg cagctgcgcg ccgaagcgct gcgccggctc ggcgacctca
     1801 acatggaagc cggcgagggc gaacgcctgg cgggtgaagc gtccaccatc gacatgcagg
     1861 gcgccgaagc catcaagctc tactcgagcc tgctcaagag ttatcccgac tatccgcgca
     1921 acgaccaggt gctctaccag ctcgcgcgcg cctacgaaac cacgggccag ccggaaaagg
     1981 cgctgtcgac tctcaacgaa atcctccgca agtatcccaa ggcgccgcag ctggacgagg
     2041 tgcaattccg ccgcggcgaa ctgctgttct cggcccggga ttaccgcagc gcagagagcg
     2101 cctatcaata cgtcgtcaac aagggcgaca aggccgcgtt cctgtcgcag agcctgtaca
     2161 aacaaggctg gtcgctgttc aagctgggcc tgaacaacga aagcctgcag ccattcgcgg
     2221 gcgtgctcga ccgcaccatg ctgcagaagg acagccgcgc gctcatcccg ctcgacaaac
     2281 tgccacgcgc caatcgtgag ctggccgacg acacgctgcg tgtcatggcg gtcacgtttt
     2341 cctatgacga cgactcggtc ggcgccatcg acacgttcct ctccgcgcac ggtaacccgg
     2401 cgtacgcgcc catcgtctat tcgcgactgg gcgacctgta tgtcgagaag gagcgcttcc
     2461 aggatgccgc ggcggtgtac cgcgcctacg cgacgcgcga gccgaacagc gagttttcgc
     2521 cgggcctgtc catgcaggcc atcgacgcgt atcgcaaggg cggtttcacg gagctggtgc
     2581 tcgagggcaa gcgcgaatat gtcgcgctgt acaactacgg cacgccgttc tggtccggcc
     2641 gcaacaaggc cgactacccg aacatcgcca aagagctcaa gaccaacctc aaggacgtgg
     2701 ccacctattt ccacgccgag gcgcagaaga cgaagaaagt ggaccagttc cgcgaggccg
     2761 cgcgctggta tcgcacctac cttgaatcct tcccggacga tccggaatcc ttcggaacta
     2821 actacctgct gtccgaggcg ctgtactcga gcgaggacta ccagggcgcc gccaccgagt
     2881 tcgccaggac ggcctataac tacccgcgca acccgcgctc ggccgccgcc gcctacgccg
     2941 cgctcggttc gtatgcgaag tacgaggaaa aactgccggc cgctgaaaag gcggaatcgc
     3001 acaagctggc cgtggatgcc ggcgtcaagt tcggcaccag cttccccgag catcccgaca
     3061 gcgcgggcgt gctgacgcgc gccgccgaag acatcttcgc cacgcaggac ctgcagcgtt
     3121 cgatcgaagt agcgaacctc gtgctcgcgc accagccgcc ggccgatcag ccaaaacgcc
     3181 gcatcgccta caccatcatc ggccaggcca acttcgatct gttgcagttc tccgaagccg
     3241 aaaagggcta catcgccgcg cgcgacctgc tgccgcccaa cgacaagatg cgcgccgacc
     3301 tcacggagcg cattgcatca gccgtgtacc gtcagggtga agccaagcag aaggccggcg
     3361 acaacctggg agccgtcgac gacttcctgc gcatctcgca ggtggcgggc acttcgaaga
     3421 tcgccgcgca ggccgagtac gacgcgggcg cacagctcat cgtcctgaag gaatggccgc
     3481 gcgccatcca ggtgctggag cacttccgcg ccaacaaccc caagagtgaa ttcagcgccg
     3541 acgtcaccag gaagctggcg gtggcattcg gagaaaccgg ccaggccgga caggccgcgg
     3601 tggaattcga gcgcatcgcg atgaacccgg ccgagtccaa agacattcag cgcgaagcca
     3661 acctgcaggc cgccgagctc tacacgaagg cgggcaacac gcccaaggcc gtcggcatgc
     3721 tggagcgctt cgtcgtcacc tatccaacgc cagtgtcgga ttcgatcgag gcgcgctgga
     3781 aactcgccga gatctcgggc accgcgggca acctcgaaag gcagcgtacc tggcagcgtg
     3841 aaatcgtgaa tgcggatcgc accgcgggtg cgggccgtac cgatcgcacg caatacctcg
     3901 cggcgaagtc gcagctcgcg ctggcctcgc cggcgcgcga tgaatatcgc agcatcgcct
     3961 tggtgctgcc gctcaagacc agcctggcga agaagcgcaa ggcgatggaa gccgcgctcg
     4021 gcggttacaa ggccgcggcc gactaccgcg tggcggaagt caccaccgtg gccacctacg
     4081 aaatcgcaga aatctaccgc cagctcggca aggacatcat gaagtccgag cgcccgaaga
     4141 aactcgcggc cgagcagctc gacgaataca actcgctgct cgaagagcag gcctttccgt
     4201 tcgaagagca ggcgatctcg acgcacgaaa tcaacaccaa gcgcgtgcgt gacaacgtct
     4261 atgacgatgg cgtgaagaag agtttcgcgg cgctcgccga gctcaagccg ggccgctacg
     4321 gcaagagcga agtcatgagc gcttcattcg atgcgctggt gccgccgctg ccacctgcgc
     4381 cggcgcttcc ggatccaccc atcactacct ccgccgatcc caacgcgaaa ccggtgagcc
     4441 ctgcgaccgc agcgcctgcc gtgccggcgt tcaatgttcc caagccgccg gcgcgcgcca
     4501 acaccgagtt cacccgcgct ctcacattga tgcgcggcag cgatgccacg caggccgttc
     4561 tcgaaatgca ggtgctgacc cagtcatatc cggacctccc ggggccgtac gcgaacctgg
     4621 gtgtactgca tcgcaatgcc aaccagctgg ccgaatcgga agccgcgctg gccaaggcca
     4681 ctgaacgcgc gccctgggat gcgcagacct ggaccgaata cggcatcacg ctgcgccagg
     4741 cgggcaaatt cgccgaggca cgcgccgcct acgaacacgc catcaaggcg aacccttcct
     4801 acgccccggc gcaccgcaat ctgggtgtgc tgctcgacct gttcctcgaa gatccgctca
     4861 ctgcccaatc ggagttcgag acttacaaga cactgacggg cgaagacaaa ccggtcagcg
     4921 gctggctggc ggaactgcgg gctcgcaaca tggtgcgggc gcacgcatcc gaagctgccc
     4981 cggaatctgc atccgagccc gccccagaat ccgcaccccc acctgaaaat gatgccgcgc
     5041 ccaaacctca aggaggcgca acgtgaacaa actactattc gtggctctgc tgttgttagc
     5101 aggcgctgcg ctcgcgcaga cgcctgctcc cgcgcccgcc acgccgccgc aggatgcgat
     5161 ggaacctggg gtgaatacct cggcaccggc cgccaaggcg cccgacgcga tggagccggg
     5221 cgtcaacact tcggttccgg ccgccgcccc ggcgaagccg gccgcgacca ctaacaaacc
     5281 tgtcgccaga cccggcgccg ccaaaagtaa tgccaaggat cgcctggacc tcgagtccac
     5341 gcagatcacc ggcaatcgcg aactgcccaa ggtgctctac atcgtgccct ggcgcagtgc
     5401 ggaaatgggc gatctcgttg gccgcccggt aaacagtctg ctcgacgagg tgctggagcc
     5461 ggtcgatcgt gacgtctttc gccgacaaaa ccgctacttc gaagcgttgc agcctaatga
     5521 ggcagcgcct gccagtaatc ctgggaagtg attaacagtt tttgttgctt atgtaacgca
     5581 atcttgtcct gcttgccccg ctcaagcccc tgagcatgtt cgttttctga ggagagtcga
     5641 ccgtgggttt catcaatacc gtcatcgtat ttttcaagga aggcggattc ttcctgtatc
     5701 cgcttctcat catattcgtg gtgggcgtcg ccatcgctat cgagcgctgg ttgtacctga
     5761 ccgtcgaaac gtcgcgcaat cgttcgctat gggaggacgt cgcgccgcat ctcggcgccg
     5821 gcaacttcaa gcaggccatc acgctcgcga acaattcgca ggcgcagatc ggcaccgtgc
     5881 tcaagtacgg gctcgcgcgt ctttcgacgg cgcggcgccg tgacgatatc cagcaggcga
     5941 tggaagagtc gctgctcgaa atcgtcccga ggctcgaaaa gcgcactcac tatctgtcgg
     6001 cgctggcgaa tatcggcctg ctggtcggtc tgttaggcac catactcgcg ctcatccacg
     6061 cgttcggcgc ggtgtcgcag gcgaacccgg ccgagaaggc ctcgctgctc gccgcggcca
     6121 tctcggaggc catgaacaac accgcttcag gtctgttcgt cgccatcgtg ttgctgctcg
     6181 cgcacatgtt cctggaatcc aagacgacct cgctgatcga cagcctggaa atcgcggtgg
     6241 tgaaattcct gaattccatc accgagcgtt attcggagcc agccgcaccg gctccgcagg
     6301 gcggcatggc tccgcgtgct ccggctccgc gacctgcctg atagctagtc gatttccgca
     6361 ctagcaacca gcagaggaac cgacgatggc ttcatccaag ctcgcagccc gttcgattcg
     6421 caatcacgcg aagtacaagg gtcgcaacga tctgaacatc gtgccgatga tcgacatgat
     6481 ggtcattctc gtgttcttcc tgctgttcac ggcggtgttc tccaacacga acatcctcga
     6541 gctcaacctt ccggcgccca actcctcggt gccggaactt cctcaggggc tccagctcga
     6601 ggtcatcgtt cgcaagaaca gcattgagat cgccgatcgc aattcaggcg tgctgcgctc
     6661 cctgccgaag aaggaaggcg agtacgacta cgcgggcctg tctgaattcc tgcagttcgt
     6721 gaaggcaaag taccccacgg tccttgccgc gaacgtgctg ctcgaacagg acatcccgta
     6781 cgacgtgctg gtgcagacga tggacaacac acgcctgtgg ctcaacgcca aaggcattgg
     6841 cctcgtcaag gtcgagctgt tcccggacat ctcggtgggt gacgcgcccc tcgtcggcgc
     6901 agccgctgca tcggtgcgag gtggaaagtg acaccgtacc gcatcaagaa acaccgccag
     6961 cgcggtcatc acgtgagcca catggcactc gtgccgttca tcgacatgct gatgatgctc
     7021 gtggtgttcc tgctgttgca cagctcggac gtcgaaatcc ttccgaacac gaagcagatc
     7081 accattccgc agtcggtcgc ggaactcaag ccgcgtgaga ccgttgtcgt gatgttgacg
     7141 acggacgcgc tgctcgtgaa tggccgccag atcgccacga tggccgagct gcgcggcgac
     7201 ggcaaacaac tcatcatcga gccgttgcgg ctcgcgctca aggaacagtc cgattcgagc
     7261 ctgcgcgctg ccgctcaggg cgatctcgcc gagcgagaag tcaccatcat gggcgacaag
     7321 ggcacgcctt tcgcggtgct caagcgcgtg atggcgacgg ctacggcggc cgactacggc
     7381 aaggtttcgc tcgcggtcat ccagcgtgaa gtcgaagcgg gggccggcgc atgagcatgg
     7441 gtctcgctcc gtacttccgc cgcttcgaac ttccgtggga cctgggagag gagaccgagc
     7501 gccgcttcct caagctggtg cgcatcctgc tcgtcatctc gaccgtgctc gccatcatcc
     7561 tgttccttct gccgatgccg aatcgcgagc gcgtgaagcc atccgacctt ccagagcgcg
     7621 tcgtgcagct catcgttgaa aagccgaagc cacctccgcc acctccgccg ccggagccgg
     7681 agaagaagcc ggaaatcgaa aagcccaaac ccacggagca gaagcccgtg gagaagccga
     7741 aacccaacgt gcgcaaccag ggcatgctcg cactgcagga cgaactcgag gcgctgcgtg
     7801 accagaacct cgatctcgcc gatccgcaga tgaaagcgcc ggtcgcggac gcgcgttcgg
     7861 agcgcaacct cattacgtcg caggccggca aggcgagcgg cggcatcaat accgccaaca
     7921 tggcgcgtgg cttcggcgga ggcgccggcg ccatcggcac gcactcaacg acgcaggtca
     7981 gtcacggagc cggcctcgat ccgaatgcgg gtgggcgcgt gcaacgcagc ggcaacagcg
     8041 gcaaagcttc gcgagcacgt gaagaagtcg agatcgtgtt cgatcgcaac aagggtgcgc
     8101 tgtatgcgct ctatggccgc gcgctgcgcg atcagcccga acttgcgggc aagctggtgc
     8161 tcgaattcac gatcgcgcct tcgggtgaaa tcaccatgtg ccgtgtggta tcgagcgagt
     8221 tgaaagatcc ggagctcgag aagaagatcg tggcgcgcgt gcgcctcatc cgcttcaagc
     8281 cggcggatgt cgagccgttg accgtcagca agcccatcga cttcttcccg gctgcctgaa
     8341 gccaaggaga atcggccatg aacctgcagt ctgaaatcgc ctactacacg gagattcgga
     8401 ttgcggacaa ggcaaggctg atggcgagtt tcctggccga gctcaccgcc gaggcgcgag
     8461 ccacgtacgg ggccacgcca gactcggtgc atgacgcggc acggctgcga ttcaccaatg
     8521 agatgtgcaa tcgcatcagc aagctcatcg agcaatacct gggtgacgac aagactcgcc
     8581 cggccgacga cgtggtgttg cgcatgcttc tctcgacacg cgccgacaag gtcgccgaga
     8641 agatgattca tgccgcctac cggcgcgcgc tgcagaactt cgagcgatat gacaccacgg
     8701 tgttcatgaa cagctagacg atggtctaac ttccacgcgc tcccgcctcg agacgcgacg
     8761 caaaccgtcg gcatcagata cgccgggtcc gccgaacaac ttcctgtgaa gtctgatccg
     8821 tccccgctgc cgggctttcg acgggcgcca ccagcagcga tgttccaggt gtcagctcca
     8881 gcgtcgaaag gtattgcgcc aatccagccc tgggacgctc tccgaaccca ttgttgtgtt
     8941 tttcgcgaca cagaaatgtc gttcacgtat taccaatcca ataccaatat tggtttcgcc
     9001 tgatgagcgt ggccaaataa ccttctgaaa aacaatggaa atacctgcca ggcaaatatt
     9061 ggttacaaac tggcttggca tttttgactg attggtacta tattggcccg caattggtcc
     9121 cgtgacaaaa caacaggggt agggaatctc tatgaacatc agacgatcga ctttgatcgg
     9181 tacggcgatt gcgttcgctt tgtacaacca gaccgcagcg gcccagacag ccaccacaga
     9241 tgctggcgag gaactcgacg aagtcatcgt ctcgggcatc cgcgcgagtc tcgagcaatc
     9301 gcttgaagtg aagcgcaatg ccgacgcgcg cctggaagtc gtgacggccg aagacgtcgg
     9361 caagctcccg gcacacaacg tggccgatgc tctgcgaggc ctgcccggcg tgaacatcag
     9421 ctcgtccagc gccgatgaag gcggcttcga cgaatccgac cgcgtcagtc tgcgtggcac
     9481 cagcccgagc ctcacccaga cgctcatcaa cggccacagc gtcggttcgg ctgactggtt
     9541 cgtgctgagc cagggcaaca acgtcggacg cagcgtcagc tattcgctgc tgccttccga
     9601 actcgtcaac tcggtggaag tgaacaagtc ctcccacgcc aggttgcagg aaggcggcac
     9661 caccggcacc gtcaacatcc tcacgcgcaa gccgtttcaa ttcgacaagt cgctgaccgt
     9721 cgaggcttcg ctcggcagtg tgtattccga ccaggcggat tcctacgatc cgcagctgtc
     9781 gggactgatc aacttcaaga acgagagcga gaccttcggt gtgttgctgc aggtgttcaa
     9841 gcaggaacgt tcactgcgcc gcgatgcgca ggaaattccc ggcggcttct tcacgatcgc
     9901 agacacagac ccggccggca tatcgaatcc ggacctcgtc ggagtgcagg tccctggcct
     9961 catcggttcg acactgttcg aaatggaacg caatcgctcc ggtggtctgc tggaactgca
    10021 gttcaagccc tcggacaacg tgaccctggg attgagcggc ttcctctcgg acatggacgc
    10081 caacaactac aaccgcaact tcatgctctg gggcggtaac tttgcgcgcg cccaggcgcc
    10141 taacccgggt tacacggtcg agaatggcgt gctgacgagt gcgacctatg ccggagtggc
    10201 cgggcgcgac tacgcggttt acgacatgat ctcccgcgaa gccacggcga agagcaacta
    10261 cgtcaccttc gatgccgact ggcgcatcaa tgactcattg acctccaggt tccaggcagg
    10321 aaccaccgag ggcgagggct cgacggctcg ccagttcatc gccgaagtga ccacgaaccg
    10381 tggtggcggc gccagctgga ccagtcacgg caccagtcgg ccactggact ggaccgttgg
    10441 cggcgacagc aacccgaccg gcgtgaccgg tttcggcacc tggggcaatc agcaggtcac
    10501 cgcgctagat agtgaagact ggttcaacct ggatttcagc aaggacctgg acaccggtgc
    10561 cctgacgtcg atcgacttcg gtgcccgctt cgccgaccac gagcgtgaaa cccagtcgcc
    10621 ggaaggcgcg agccccggtg acatctggtc cgatctgcag agcggcgata ccaacctcta
    10681 cccgagcgac ttcgccgccg acatcgacgg atccttcccg cgcggcctgt ggttcttcac
    10741 gccgaatgcg ctgagggagg cggtccttgc caactccacc tggctgtcgg acaacgacgg
    10801 cccgaccggt cgccacaact acggtggtga atggaaggtt tccgagacga atctcgccgc
    10861 ctacgtgcag gcgaacttcg ccggcgaagc ctggagcggc aacttcggcc tgcgctacgt
    10921 cgacatcgac caggagatca actcctacca ggcggtctcg gccacgtcgg acgttatcga
    10981 tgtgagcagt ctcttcggca actggatcta ccaggacacc aacaacaagc acagcaaggt
    11041 gctgcccagc gcgaacctga agttcaacat gggtgaggat gtggtgttcc gcttcgccgc
    11101 gtcgcagacc atgaccctgc cggactattc ggcactgggc gctgccagct ggggttcgga
    11161 tctgaacagg acgggcggcg gcggcaaccc gaacctgcag cccgtgacct ccaccaactt
    11221 cgatggcaac ctcgagtggt atttcatgcc gcgcggcctg gtgtcggtcg gtgcctttgc
    11281 gatggatctg gatgactaca tcgccttcgg caccgagaca cggatgttgt acagcgagct
    11341 gaccaatcag ctcgaggcgt acaacgtcgc ggttcctgtc aactccaacg gctcggtgac
    11401 gggcctggag ctggtgtacg agcagcccat cggtgaatcg ttcggcttca acgccaatta
    11461 cacctacgcg gatggcaaga cggctcacac gtgggccgac ggcagcaaca atctggtggg
    11521 cacctcgaag gacacctaca acgtgggcct ctacttcgag aatgagatgt tctcggcacg
    11581 tgcgtcctac tcctatcgca ccgcgttcct gatcggcctg tcgggcgcca acccgtatta
    11641 ccaggacgat ttcgggactg tgtcagtggc gctcaactac aagcccaccg actggatcaa
    11701 tgtcagtttt gacgcgttga acctcaacgg cccgactctg acttactacc agagtgcaac
    11761 ggcccccacg gcgttctaca acaacggcaa acagtactac ctcaaccttc gcttcaagtt
    11821 ctgacgctta gagtcttgag cgaacggccc gcagtccctc cccctgggac tgcgggcctt
    11881 tttcctgaat gggtagttag ttagggcttt ctgtggttca gaagacgagc gtctgaatcg
    11941 agcggggcgg gctcttgacc gccgccgcct gcccatcgac ccagagaaaa tactccactt
    12001 ccttttcgcc gccgttcatc accacgactg ccaccttgcc atccccgttg acgaaggccg
    12061 tcgagagaag ggcgcttcgg ctcggagagc tcgcgatacg cttcgcgccg ggccgcacga
    12121 acttcgagaa gtgcccgatg tagtaatacg agttcgtgta gatgagctcg ccgctcctcg
    12181 tgtccgcgtg catcggtgca tagcagtaat tcgcgacgtg gttggggccg cctttctcat
    12241 cgaggaggat gttccagtcg caccagcccg ccatgccgtt gttgaaatcg ttgatcatct
    12301 gccgggcgta tctctcgcca agcttccaat caccgagctt gtcgaagttg aaagcttcca
    12361 cgcagccctc ggtgaagatc agcggcttgt cgggatacgc gtcgtgcgtc agcttcacgt
    12421 tgtcgtgcat cggctcgccg ccgctccagg tctcgtacca gtggaagccg ataccccagg
    12481 cgtaccgggc ggcatccggg tccgcgagga tcgtgctgac tcgctgatag atgaggtcgc
    12541 gattgtggtc ccagacgatg atctttttat cgccgagccc ttcctttcgc agcgtcggcc
    12601 cgaggtagtt cttcagaaaa tcccgctcgt cttcggcgga gtagatgcag gactcccacc
    12661 tctgcgtcgc catcggctcg ttctggacgg tcaagcccca gacgggtatg ccctcggcct
    12721 gatacgcctt gatgaacctg gcgtagtagt tggcccaggc ctggtagaag tccggtttga
    12781 gctttccgcc cttgagcatg ctcccgttgt ctttcatgaa cgcgggtggg ctccaggggc
    12841 tcaccaggaa cgtcaacttg ccgccggccg tttcggccgc ccgtttgatg aggggaatgc
    12901 ggtagcgacg gtcgggcgcg acgctgaatg tcttcagttc cttgtcgccc tcggcgacat
    12961 acgtgtagct cccgctcgag aaatcgcagc tatgtatgtt cgtccgcacg aacgtgtacc
    13021 cgattccctt ttcgcggtgg aagtaggcgt cgagaaactc gcgctgctta ccctccggga
    13081 tcctggcgaa ggtttccgcc gaagagtccg tgatcgctcc gccgaagccg atcgtcgcct
    13141 ggtaggtctt cgagggatcg acgaagacac agacctgcgt ttcgaggggc tgtccgaaat
    13201 gcttgaaatc gagcgtgccg gttactgaaa gccggtgatc cgacttgtcg gcagtcgtgt
    13261 aaacggtgac ctgcctcccg tcgacggaga attttccagg ggcggcggcg cgccgatttt
    13321 gtgcggaggc gggccgatct gccgacgcca gaattccagc ggaagcggat gcgacaagga
    13381 gtttgcggcg gtcgattttc atgccgaaac gctataccac attcgtacta ggttttcttc
    13441 agccagagca gccccaacaa acctgcgatg agagatgctg ccagtaccgc gagcttcgaa
    13501 gaattgacga tggccgcgtg atccacgaac gccaggttgg tgatgaagat cgacatcgtg
    13561 aatccgatgc cgccgagcag tcccgcgccc accatgtgag gccagcggac gttcgcgggc
    13621 agcgagcaaa cgcctgtggt gaccgccagc acgcacatca cgatgactcc gagcggtttg
    13681 ccaactatca atccgagcgc gatgccgatg ctgttgacgt ggctgagctc cgcgatggat
    13741 tcctggttga tgggcacacc cgtgtttgcg agcgcgaaca gcggcaggat caggtacgcg
    13801 accggcttgt gcagcgtccg ctccaggcga tgtgaaggcg actcggcctc cgatgttcgc
    13861 ggcgagaacg gaatggcgaa tgcgagcagg acgcccgcga tcgacgcatg aacgcccgcc
    13921 ttgtacacgc agagccagag gaacacgccg ccgatgagat acggccacag cggcatgacg
    13981 cgcagcggac agttgagaat gatgagcgct gcccacgtgc ccagcgccat tgccagccac
    14041 gtgaacgaaa ggtccgccgt gtaaaccgtg gcgatcaaca cgatggcccc gaggtcatcg
    14101 atgatcgcga acgccacgat gaagagcttc agtgcgaccg gcacgcgcgg ccccaccagc
    14161 gcgatcacgg cgagcgcaaa cgcgatgtcg gtggccatgg gaatgccgaa gcccgattcg
    14221 gtcgctgtgc cgccattgaa tgcgaagtgg atgagcgccg gagcgaccat gccgcccacc
    14281 gctgcgaacg ccggcagcag cgcgtttcgc agcttcgaca gcgccccgat atagatgccg
    14341 cgttcgagct cgagaccgat catcaggaaa aaaatcgcca tcagcgcgtc gttgatccag
    14401 tgctcgacgc tcagcgggcc caatttcagg tgccagagcc cgagccaggg cgcggcaagg
    14461 ggcgagttgg cgagcaccag tgacacgatg gtgcacccca cgagcaggac gcccccgaat
    14521 tgttcggatt gaacgaaccg cctgaaggtc tggtgcagct gcatggctgt gctaacttct
    14581 cacgtgaatg actgaatcgc cactatccct catcctcgta ctgctggccg cgtcggtatt
    14641 cgtcgtgacg ctggcacggc gcctcgggct gccgtcgatc ctgggctacg tcaccgtcgg
    14701 actggtgctg gggccgcacg ccctcggcct gttccccgag tccgataccg cccatctgct
    14761 ggctgaactc ggggtggtct tcctgttgtt cacgctcggc ctcgaatttt cgtggccgcg
    14821 aatggtggcg ctgcgccgcg aggtgttcgg cctggggtca ttgcaggtat tcggcaccgc
    14881 gggcgtggtc gcggtcattg cgcacctgtt cggactccag tgggcgcaga gcatcgtgat
    14941 cggtggcgtc gtggccatga gctccacggt gctcatcgtg cagctgctga cggaacgtgc
    15001 cgagctcaac cgcacgcacg gccggctcgc cttcagcgtg gtgttgttcc aggacatcgc
    15061 cgtggtgccg ttcctggcac tggccgcggc gctggctccg ggtagtgagg aattcagtta
    15121 cgccggtgcg ctcagcctgg ttgccgccgg aaccctggca gtgtgcgtcg tgctggcggc
    15181 gggccgctgg ctcctgaagc cgctgctcta cgtcattgcc aacagccggc tgcgcgagct
    15241 gttcacgctc gcggtgctgc tggttgcact ggcctcggcc tgggcatcgc aaatggccgg
    15301 cctgtcgatg gcgctcggcg cattcctggc gggcatgatg ctggccgaga ccgaatacag
    15361 ccaccaggtc gaaagcgtca tccggccgtt ccgcgacatc ctgctcggcc tgttcttcat
    15421 ctccgtgggc atgctgctcg acttcaatgc gctgtggcgc gagttgtggc tgatcctcgc
    15481 tttggtcgtg gccatcgtgg tggtcaaggc ggtggtcgcg agtttcgcca cgcgcctgtt
    15541 cgtcgactcc aacttcaagg cgcttcgcac cggcgtcacg ctcgccgtgg gcggcgagtt
    15601 cggtatcgca ttgttgacac tgctgttgca gaaccaggcc atcgatcctg ccatcgcgca
    15661 gccgttgctg gtcacggtgg tccttggaat ggtgctggcg ccggtgatcc tggccaacaa
    15721 caaacgcgtg gcgcggctgt tgttgggcga acgcggcccg ccgaagaccg ctgtcgaacg
    15781 cgaagacgcc gcgacgcagg cggtggcgaa acgcgagcac gtgatccttt gcggcttcgg
    15841 ccgcgtcggc cagaacgtgg cgcgtgtgct gg
//
LOCUS       DASQJS010000258         6401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_151854, whole genome shotgun sequence.
ACCESSION   DASQJS010000258 DASQJS010000000
VERSION     DASQJS010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151854"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1518
                     /locus_tag="VEW08_10420"
     CDS             82..1518
                     /locus_tag="VEW08_10420"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYJ41198.1"
                     /translation="MLQTLSVRTVLLLASVVAMTSMATAAGSKSRAGEAEFREVYKEL
                     VETNTALSHGSCTLAAERMAARLKSVGLEQVQVFTTPDHPREGGLFAVFPGSDPKAKA
                     MLLLAHIDVVEAKREDWTRDPFVLVEENGSFYARGAVDDKAEAAIWVDTFVRYAREKF
                     KPRRPIKLALTCGEETAGAFNGAEWLAKNHRDWIDAEFAINEGAWGEMDAQGNRLVMN
                     VQAGEKAAQDFRLEVMNPGGHSSRPRKDNAIYLLASALSRVEAYEFPAQFTDASRAYF
                     SGMAKIQAAKGNAEVAAAMNALVKDPTDAKAIALVSARDVSWNATMRTTCVATMLEGG
                     HATNALPQRARAIVNCRIFPGVSAESVRAQLEKLVANPEVKVTMPGTREPASPPPPLS
                     SKIMAPLEKLTAQYFPGVPVLPILQAGATDGLYLNGAGIPTYGILPLFMGPDLGNIHG
                     LNEYVGVQSLLDARAFLYDLVKIYSTQK"
     gene            complement(1519..2220)
                     /locus_tag="VEW08_10425"
     CDS             complement(1519..2220)
                     /locus_tag="VEW08_10425"
                     /inference="COORDINATES: protein motif:HMM:NF039702.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6250 domain-containing protein"
                     /protein_id="HYJ41199.1"
                     /translation="MSSTASWRTSASATFALLILTGCATPAPGELLYQDDFKDLANWH
                     IEAEKPGRISAADGVLDIDVPAGVTLWLERYLEGPLVIEFEATAVSEGGPNDQVSDLN
                     VFWMANNVDGQQPVFSRTRSGAFADYNNLLTYYVGLGGNRNTTTRFRRYIGDPVLRPL
                     LPEHDLSLASAMLVPNRRQTVTLIADGSRIEYRREGEPLFRIDDAAPYARGWFALRTT
                     YSHLRIRNLRIYRLP"
     gene            complement(2186..3115)
                     /locus_tag="VEW08_10430"
     CDS             complement(2186..3115)
                     /locus_tag="VEW08_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009402535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HYJ41200.1"
                     /translation="MSQKKPVMGRGLEALLGQMSKRPEPAPEAGSAAPPPQAKLPGDE
                     LANLPLDLLQRGKYQPRVDMRQESLEELAASIRSQGVIQPIVVRAVDGAAPGASQRYE
                     IIAGERRWRAAQIAGLATMPAVIRKVPDEAAIAMALIENIQREDLNPLEEARALERLI
                     NEFGITHQQAADAVGRSRAAVSNLLRLLELAPEITAFVERRELEMGHARALLALTQRR
                     QQVEVGTLVAKRQLSVRDTEGMVRGILARASGGSAPKDSKPLDPNVQRLQDELTEKLG
                     APVQIQHTGSGKGKVVVSYHSLDELDGILAHIR"
     gene            complement(3184..4035)
                     /locus_tag="VEW08_10435"
     CDS             complement(3184..4035)
                     /locus_tag="VEW08_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HYJ41201.1"
                     /translation="MKRIIAVANQKGGVGKTTTAVNLAASFAATRRRVLLIDIDPQGN
                     ATMGCGVEKSQITRGTGDVLLGEAEAASVIINLEAIPGLALMPSNQDLTAAEVRLLTT
                     MAGRELKLRNALRPIYDQYEVILIDCPPALNMLTLNALVAAQSVLIPMQCEYYALEGL
                     SALVGTIEQIKAAVNPNLEIEGILRTMFDPRNNLANEVSSQLITHFGDKVFRTIVPRN
                     VRLAEAPSFGKPVLQHDKDSRGALAYLALAGEMLRKDDDAANDVEVVPAAEAAADGME
                     MGSGTVS"
     gene            complement(4075..4746)
                     /gene="rsmG"
                     /locus_tag="VEW08_10440"
     CDS             complement(4075..4746)
                     /gene="rsmG"
                     /locus_tag="VEW08_10440"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HYJ41202.1"
                     /translation="MQTDVTGTNNFDEAGWKGKLAAGAAELKAPLDDVQLDKLWRLGL
                     LLRERNEHVNLTSIVSPEGILTLHMLDSLSIVPDLLDAATVLDVGTGGGFPGLPLAVA
                     CPQREFMLIDGTQKKIRFVNEAIEALGIPNAKAAAVRAEDLDEPKYDAVIVRAVGSLI
                     DVLHNAGHLVAPEGRLIAMKGRHPAEELRTVPRGWKAKVSVLRVPGLDAERHVVALTR
                     QKRAQ"
     gene            complement(4736..6184)
                     /locus_tag="VEW08_10445"
     CDS             complement(4736..6184)
                     /locus_tag="VEW08_10445"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:TIGR02330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HYJ41203.1"
                     /translation="MSSHDYKSAQRPNPDELLVAIADYTRNYQVDSAQAYETARYCLM
                     DTLACGFQALKYPACTRLLGPVVPGATMPGGSRVPGTSYELDPVMGAFSIGAMVRWLD
                     FNDTWLAAEWGHPSDNLGAILAVSDYLSRRAILLGEKPLTVRDVLTAMIKAHEIQGVL
                     ALENSFNRVGLDHVLLVRVASTAVATAMLGGSLEQIVNALSNAWIDGGALRTYRHAPN
                     TGSRKSWAAGDATSRAVRHALFALKNEMGYPSALSAKTWGFQDVLFKGKTLTLAQPLG
                     SYVMENVLFKISFPAEFHSQTAVECAMALHPQVKDKLGLIERIVIETQEPGVRIIDKT
                     GPLANPADRDHCIQYMVAVPLIFGRLTAADYEDSVASDPRIDLLRSKMEVRENTTFTK
                     EYYERDKRHIGNALHIFFSDGSPKLELRIDFPVGHRKRRAEGMPLLVQKFEASVAAHY
                     PARQTEAIKALFARRDLDAMPVNEFMAALVAN"
     gene            complement(6192..>6401)
                     /locus_tag="VEW08_10450"
     CDS             complement(6192..>6401)
                     /locus_tag="VEW08_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HYJ41204.1"
                     /translation="FPNLDWYSAVSYHLMGVPTAMFTPLFVIARATGWAAHVIEQRED
                     GKIIRPAANYIGPENRKFVPIEQRH"
BASE COUNT         1080 a         2017 c         2121 g         1183 t
ORIGIN      
        1 gacgcgattt ttcctgggtt gttttacgta ttcgccgccg gcaaccgtag tatccggcgc
       61 agcaccagcc aggaaatttc catgctccaa accctcagtg tccgcaccgt cctcctcctt
      121 gcttctgtcg tggcgatgac atccatggcc accgcagccg gatcaaagtc gcgtgccggt
      181 gaagccgagt ttcgcgaggt ctacaaggag ctggtcgaaa ccaacacggc gctgtcccac
      241 ggcagctgca cgctggcggc ggagcgcatg gcggcccggc tgaaatccgt ggggctcgag
      301 caggtgcagg tcttcaccac gcctgaccat cccagggaag gcgggttgtt cgcggtgttc
      361 ccgggcagcg atccgaaagc gaaagcgatg ctgctgctgg cgcacatcga cgtcgttgag
      421 gcgaagcgcg aggactggac gcgcgacccg ttcgtgctgg tggaagagaa cggctcgttc
      481 tatgcgcgcg gcgccgtgga cgacaaggcc gaggctgcca tctgggtcga caccttcgtc
      541 cgttacgcgc gcgagaagtt caaaccgcgc aggcccatca aacttgcgct gacctgcggc
      601 gaagaaacgg ctggtgcctt caacggcgcg gaatggctcg cgaaaaacca ccgcgactgg
      661 atcgacgccg agttcgcgat caacgagggt gcctggggcg agatggatgc gcagggcaac
      721 cggctcgtca tgaacgtgca ggcgggcgag aaggctgcgc aggacttccg gctcgaagtg
      781 atgaatcctg gcggtcacag ctcacgaccc aggaaggaca acgccatcta tctactggct
      841 tccgcgttgt cgcgcgtcga ggcttacgag tttccggcgc agttcaccga tgcaagccgc
      901 gcgtacttca gcggcatggc gaaaatccag gctgcgaagg gcaacgccga agttgccgcg
      961 gcaatgaacg cgctcgtcaa ggatccgacc gacgcaaagg ccattgcgct ggtgtcggcg
     1021 agagatgtga gctggaacgc cacgatgcgc acgacctgcg tcgccacgat gctggaaggt
     1081 ggccacgcca ccaacgcgct gccacaacgc gcgcgcgcca tcgtcaactg ccgcatcttc
     1141 ccgggcgtat ccgccgagtc cgtgcgtgcg caactcgaga agttagtcgc caatccggaa
     1201 gtgaaggtca ccatgccggg cacgcgcgaa ccggcttcgc cgccaccgcc gctgagctcg
     1261 aagataatgg cgcccctcga gaagctcacc gcacagtatt tccccggcgt acccgtgctg
     1321 cccatcctgc aggccggcgc caccgacggg ctgtacctga atggcgcggg aattccgacc
     1381 tacggcatcc ttccgctgtt catgggtccg gacctcggca acatccacgg tctcaatgaa
     1441 tacgtgggcg tgcagtcgct gctcgatgcg cgcgcgttcc tctatgacct cgtgaagatc
     1501 tattccacgc agaagtagct agggcaggcg gtagattcgc aggttgcgga tgcgcaggtg
     1561 gctgtacgtc gtgcgcagcg cgaaccagcc tcgcgcatag ggcgccgcgt cgtcgatgcg
     1621 gaacagcggc tcgccctcgc gccgatattc gatgcgcgaa ccatcggcta tcagcgtgac
     1681 ggtttgcctg cggttgggca cgagcatcgc cgaggccagc gagagatcgt gctcgggcag
     1741 caggggccgc agcaccgggt cgccgatgta gcggcggaag cgcgtggtgg tgttgcggtt
     1801 gccgccgagg cccacgtagt aggtcagcag gttgttgtaa tcggcgaacg cgccgctgcg
     1861 ggtgcgtgag aacaccggct gctggccgtc cacattgtta gccatccaga agacgttgag
     1921 atcgctgacc tggtcgttgg gtccgccttc ggacacggcg gtggcttcga actcgatgac
     1981 cagcggacct tcgagataac gctcgagcca cagcgtgacg cccgccggaa cgtcgatatc
     2041 gagtacacca tcggcggcgc tgatgcggcc gggcttctcg gcctcgatgt gccagttcgc
     2101 caggtccttg aaatcgtcct ggtagagcag ctcgcccggc gcgggcgtgg cgcagcccgt
     2161 gagaatcagc agcgcgaacg tcgcgctagc ggatgtgcgc caggatgccg tcgagctcat
     2221 cgagcgagtg atagctgacg acgactttcc ctttgccgga accggtgtgc tggatctgca
     2281 cgggggcgcc gagtttttcc gtgagctcgt cctgcaggcg ctgaacgttg ggatcgagag
     2341 gcttgctgtc tttcggggcg ctgccgcccg aggcccgcgc gagaatgccg cgcaccatgc
     2401 cttccgtgtc gcgcaccgag agttgacgct tcgccaccag cgtgcccact tccacctgct
     2461 gacggcgctg ggtcagtgcc agcaaggcgc gcgcatggcc catctcgagc tcgcgtcgtt
     2521 cgacgaaggc ggtgatctcg ggagccagct cgagcaggcg caggaggttc gacaccgcgg
     2581 cgcgtgaacg gcccacggca tccgcggcct gttgatgcgt gataccgaac tcgttgatga
     2641 ggcgttcgag cgcacgcgct tcttcgagcg gattgagatc ttcgcgctgg atgttctcga
     2701 tgagcgccat ggcgatcgcg gcttcatcgg gcaccttgcg gatgacggcc ggcatggtgg
     2761 cgaggccggc gatctgcgcg gcgcgccagc ggcgttcgcc ggcgatgatt tcatagcgct
     2821 gcgaggcgcc cggcgcggcg ccatcgacgg cgcgtacgac gatgggctgg atgaccccct
     2881 gcgatctgat cgacgccgcc agttcttcga gcgactcctg gcgcatgtcg acgcgcggct
     2941 gatatttgcc acgctgcaac agatcgagcg ggaggttcgc gagttcgtca ccgggcagct
     3001 tggcctgcgg gggcggggct gccgaaccag cctcgggcgc aggctcaggg cgcttggaca
     3061 tctggccgag caaggcctcg agtccacgtc ccatcaccgg tttcttctgg ctcatggcgg
     3121 cggatgataa agggaaatgg ggtcaggcac cgtttcctgg aaaacggggt caggcaccgt
     3181 ttcctaggaa acggtgcctg accccatttc cattccgtcc gcggcggctt cggcggccgg
     3241 cacgacttcg acatcgttgg ccgcgtcgtc gtccttgcgc agcatctcgc cggcgagcgc
     3301 gaggtaggcc agcgcgccgc gtgaatcctt gtcgtgctgc aggaccggct tgccgaaaga
     3361 cggcgcctcg gccaggcgca cgttgcgcgg cacgatggtg cggaacacct tgtcgccgaa
     3421 gtgcgtgatg agctggctcg acacttcgtt ggcgaggttg tttctcgggt cgaacatggt
     3481 gcgcaggatg ccctcgatct cgaggtttgg attcaccgcg gccttgatct gctcgatggt
     3541 gccgaccagc gccgacagcc cctcgagcgc gtagtactcg cactgcatcg ggatgagcac
     3601 gctctgcgcc gcgaccagcg cgttcagcgt gagcatgttg agagccggcg ggcagtcgat
     3661 gaggatgact tcgtactggt cgtagatggg cctgagcgcg ttgcgcagct tgagctcacg
     3721 gcccgccatg gtagttagca ggcggacttc ggcggcggtc aggtcctggt tggacggcat
     3781 gagcgcgaga ccgggaatgg cttcgaggtt gatgatgacg gacgcggctt ccgcctcgcc
     3841 taacaacacg tcgccggtgc cgcgcgtgat ctgcgacttc tccacgccgc accccatggt
     3901 ggcgttgccc tgcgggtcga tgtcgatcag caacacgcgg cggcgagtcg cggcgaacga
     3961 cgccgcgaga ttcacggcgg tggtagtttt ccccactccg cctttctgat tggcgaccgc
     4021 aatgattcgc ttcatgcggg catcttactc tgtgctctac ggcatcggag ccgcttattg
     4081 agcccgtttc tgacgcgtca gtgcgacaac gtggcgctcg gcatcgaggc ccgggacacg
     4141 cagtacggaa acttttgcct tccagccgcg cggcacggtg cgcagctctt cggctgggtg
     4201 acgccccttc attgcgatca accggccctc gggcgcgacc aggtgcccgg cgttatgtag
     4261 tacgtcgatg agactgccga cggcacgcac gatgaccgcg tcgtacttcg gctcgtcgag
     4321 atcctcggcg cgaaccgcgg ccgcttttgc gtttggaatt cccagtgcct cgatggcttc
     4381 gttcacgaag cgaatcttct tctgggtgcc gtcgatgagc atgaactcgc gctgcgggca
     4441 ggcgaccgcc agcggcagcc cgggaaagcc accgcccgtg cccacgtcga gcacggtggc
     4501 ggcgtcgagc agatccggca cgatcgacag actgtcgagc atgtgcagcg tgaggatgcc
     4561 ttcgggcgag acgatggacg tgaggttgac gtgctcgttg cgctcgcgca gcagcaggcc
     4621 gagccgccag agcttgtcga gctggacgtc gtcgagcggc gccttgagct ccgcggcgcc
     4681 cgcggccagt ttccctttcc agcccgcttc gtcgaagttg ttagtgccgg tcacgtcagt
     4741 ttgcaaccag cgcagccatg aattcgttca ccggcatcgc gtcgagatcc ctgcgcgcga
     4801 acagcgcctt gatggcctcg gtttgccttg ccgggtaatg cgcggccacc gacgcctcga
     4861 acttctgcac cagcagtggc atgccttcgg cgcgacgctt gcgatgcccg accgggaagt
     4921 cgatgcgcaa ttcgagtttc gggctgccgt cgctgaagaa gatgtgcagc gcattgccga
     4981 tgtggcgctt gtcgcgctcg taatattcct tcgtgaaggt ggtgttctcg cgcacttcca
     5041 tcttgctgcg caggagatcg atgcgcggat cgctggccac cgaatcttcg tagtccgctg
     5101 ccgtcaggcg gccgaaaatc agcggcaccg cgaccatgta ctggatgcag tgatcgcgat
     5161 ccgcgggatt cgccaacggg ccggtcttgt cgatgatgcg cacgccgggt tcctgggttt
     5221 cgatgacgat gcgttcgatg agcccaagtt tgtccttgac ttgcggatgc agcgccatgg
     5281 cgcattccac cgcggtctgg gaatggaact ccgccggaaa gctgatcttg aacagcacgt
     5341 tctccatgac gtagctgccg agcggctgcg cgagtgtcag cgtcttgccc ttgaacagca
     5401 catcctggaa accccaggtt ttcgcgctca acgccgaggg ataacccatc tcgttcttca
     5461 gcgcgaacaa tgcatggcgc accgcgcgac tcgtggcatc gcccgccgcc cagctcttgc
     5521 gcgagccggt gttgggcgcg tgccggtagg tgcgtaatgc gccaccgtcg atccacgcgt
     5581 tcgacagcgc gttcacgatc tgctcgagcg agccgccgag catggccgtg gcaaccgccg
     5641 tcgatgcgac ccgaaccagc agcacgtggt cgagacccac gcggttgaag ctgttctcca
     5701 gcgccagcac gccctggatc tcgtgcgcct tgatcatggc cgtgagcacg tcgcgaaccg
     5761 tcagcggctt ctcgcccagc aggatggcgc gccgcgatag atagtccgac accgcaagaa
     5821 tggcgccgag attgtccgac gggtgacccc actccgcggc cagccaggtg tcgttgaagt
     5881 cgagccagcg caccatggca ccgatgctga acgcgcccat gaccggatcc agctcgtagc
     5941 tcgtgcccgg cacgcgtgag ccgccgggca tcgtcgcgcc cggcaccacg ggccctaaca
     6001 accgtgtaca cgccgggtat ttgagcgcct ggaagccgca ggccagcgtg tccatgaggc
     6061 agtagcgcgc ggtctcgtac gcctgcgcgc tatctacctg gtagttcctg gtgtagtcgg
     6121 caatggcgac caggagctcg tccgggttcg gccgctgcgc ggatttgtaa tcgtgcgatg
     6181 acatcggttt ctcagtgcct ctgctcgatc ggcacgaatt tgcggttctc cggaccaatg
     6241 tagttggcgg cggggcggat gatcttgccg tcttcgcgct gctcgatcac atgcgcggcc
     6301 cagcccgtgg cgcgggcgat cacgaacagc ggcgtgaaca tcgcggttgg cacgcccatc
     6361 aggtgatatg aaaccgccga ataccagtcg aggttcggga a
//
LOCUS       DASQJS010000259         7281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1090997, whole genome shotgun sequence.
ACCESSION   DASQJS010000259 DASQJS010000000
VERSION     DASQJS010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090997"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VEW08_10455"
     CDS             <1..1122
                     /locus_tag="VEW08_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HYJ41205.1"
                     /translation="FAVASTDSGHQATGFDGTFFRDQQASLNFLYQAVAEVTVVTKAI
                     IARYYGKPQQHAYFVGCSTGGREAMMMSQRFPNYFDGIVAAAPAARTSFSNLGLRYAT
                     AALNAIAPLDADGKPQTRAALSDADRKLLIDGVLEACDTLDGHKDGLIFAPQKCQFDP
                     GTLALSAAQVNAIRAVMQGPRTAAGRQVYPGYFYDTGIANTQGLPGVLAGPVIPEGGP
                     VATRFDVDAAAGDAMDARAMVGDSSAWTNLSTFRGHGGKLIFLHGVSDPWFSAQESVR
                     YYELLGRDNTEVPLAEWSRLFLVPGMGHCGGGSRTLDQFDMLSAVVDWVESGRAPERV
                     IATGKSMPGESRPLCPYPQHAHYAGSGDAHEAANYICRQ"
     gene            complement(1127..2764)
                     /locus_tag="VEW08_10460"
     CDS             complement(1127..2764)
                     /locus_tag="VEW08_10460"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HYJ41206.1"
                     /translation="MIRNPILPGFNPDPSILRVGDDYYIATSTFEWYPGVQIHHSRDL
                     VNWRLLTRPLRRASQLNMLGDPDSCGVWAPCLSYSDGLFWLIYTDVKRYGRTTVGGAS
                     GASLRDFHNYLVTCPTIDGEWSDALYVNSSGFDPSLFHDDDGRKYFVNQLWDHRPGRG
                     RFAGIVLQEYSPAQRKLVGEPKNIFKGTSIGFTEAPHLYKRNGYYYLITAEGGTGWGH
                     AMTMARSRSLTGPYELHPDKYIITARDRPHAPLHRAGHGDLVGTQAGETYAVYLCGRP
                     LPNRGRCVLGRETAIQKMVWGKDDWLRTQDGSGLPELETPAPSGVPAAPFDSKNAAAA
                     VREDFDSKQLPIDFQWLRSPYPEELFSLSERPGHLRLFGRETLGSVFRQSLVARRQQA
                     HCYSAVTAMDFEPAHFQQAAGLVCYYNSSKFHYLHVSHDEEHGRHVRVMSCVPDLPQS
                     DFFTAPIPIPAGRIELRVEVDYERLRFAYRVGGDWKFLPELFDASVLSDECTGPGSAN
                     FTGAFVGVACQDMSGMAHPADFDYFEYREREYLQSAE"
     gene            complement(2761..4035)
                     /locus_tag="VEW08_10465"
     CDS             complement(2761..4035)
                     /locus_tag="VEW08_10465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ41207.1"
                     /translation="MPVIRRLYALRWWMISLITIGTILNYLTRATLGVAAPTLTGELG
                     IGEQEYSWITGVFQIGIMLQPFAGYVMDLIGLKLGFGLFAIAWAIITMLHGLALNWQM
                     LAALRGLMGFAEGSAQPGGMKAVAEWFPAKERGFAGGFYNIGASFGSMLAAPLVAWAI
                     LFHSWRLAFVVAGGLALVWVALWFKFYYSPKDHPKISAEERNYIESGQEAHLAAGATK
                     TSILNLLKQRNVWGIAIPRMLADPTWGTLAFWVPFYLTTERGFDLKQIALFAWLPFVT
                     ADIGCLAGPTIVLWLQRRGIGLINARRWAFTVGAALMTSMAFVGKVDSPYVAIALLCV
                     GGFAHQTLSITVITMSSDLFRRNEVATVAGIGGFCGNLGVLMFTLLIGGLVKTVGYDP
                     FFVALAVFDILAAIWLWSVVRERVTPPVATAA"
     gene            complement(4171..7203)
                     /locus_tag="VEW08_10470"
     CDS             complement(4171..7203)
                     /locus_tag="VEW08_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ41208.1"
                     /translation="MTSRAYFRASLVALGVSAALNGNSAWAQTPEQQPVEEDMGVITV
                     TGSRTITDVLKSPTPITSVDVADMALTTPSDTADALNKLPSIIGGRTPRNQGNGSTNN
                     GGNTLSLRNFGPSRTLVLLDGHRVAPSNQDGSVNIDVLPQMLVKNVDIVTGGASAIYG
                     SDAVAGVVNYMLDKNFTGLSLKGDFGMSKYSDGDQYQVGAAWGTSLFSDRGHFEVAAR
                     ARKQDMIPQHERPYAEDGQSWLQTGNGRTVASGGRPFEFTPYSRVFNSGPNGNVQCGT
                     ACAYNNYTFDAAGGLVPMVHGTPSASGGIETGGDGSYIKYGTFRSELDAKDVFARFSL
                     DVGESANWYVQGSWAQAENASDWIQWVVSPNNGRPNTLFANNPYLTPATQALLGSGIV
                     CGTPAATGWRCLPATPPTSPQTGSTPPPPPNIPIFANPRYFNDVDGLPVDGSAGGPNR
                     MYRTLGDQKQWNAETGITGEVGGFTWEAFYNHSTSELSVTNPNNTDNAKYLASLDAVN
                     DAGTIRCWVSTQPQFASLYPDCVPSNITAPGGISADAYDYLRVKTSWLLTQDLDNVGA
                     SIGGGLWGFGLPAGEITANLSVDARWSTYVLESDFLPSDFVNCTGLRMCLAAGGAAPL
                     RWVQNTVAPVDVDDHVYEGALEFNVPLLKEVPGFQDLSTNLAYRWTKYSSFDAVDSWK
                     VGLNWQIVDSLRIRSTYSSDIRAPNLNDLYQPSGVTSSSYNDRLTGGSAQGMRLASRG
                     NPLLTPEEATTVTVGFVLTPTALPRFGLSVDFWETRLEDAITNLNYANDSVQAICLSS
                     APTYSSPVCDLAVRPITDPSDPDYFNPAVNMPTEVRSAPVNAALQKIKGYDLQLDYNW
                     DIGAGNLALRHLVTHQPVNSTLTTPASTFYTWAVQPKWMQTTFLSYRNSSWSVALQNR
                     WLGSTSLKSSNNDLNGNTQNYLDSSLDANNVVDTTITKEFEVSGGTLAAFLTVNNLLD
                     ERAPLAPSGSGLPGLFYPTLAFYDDMGRFFTAGVRINF"
BASE COUNT         1405 a         2251 c         2310 g         1315 t
ORIGIN      
        1 tttgcggtcg cgagcaccga ctccggccac caggccacgg gcttcgacgg aacctttttc
       61 cgcgaccaac aggcctcgct gaacttcctg taccaggccg tggcggaggt aactgtcgtc
      121 accaaggcca tcatcgcgcg gtactacggc aagcctcagc agcacgcgta tttcgtcggc
      181 tgttccaccg gcggacgcga ggcgatgatg atgtcgcaac gctttcccaa ttacttcgat
      241 ggaatcgtgg cggccgcacc cgcggcgcgc accagcttct cgaacctggg gctgcggtac
      301 gccactgccg cgctcaacgc gatcgcaccg ctcgatgcag acggaaaacc gcagacacgc
      361 gcggcgctga gcgatgcgga tcgaaaactc ctcatcgacg gcgtgctcga ggcctgcgat
      421 acgctcgacg gccacaagga cggcctcatt ttcgcgccgc agaaatgcca attcgatccg
      481 ggaacgctgg ctctgtccgc agcgcaggtc aatgccatac gggctgtcat gcaggggcca
      541 cgcacggcgg ccggacggca ggtctatccc ggctacttct acgacaccgg catcgcaaac
      601 acgcaaggac tgcccggcgt gctcgccgga cccgtgattc ccgaaggcgg accggtggca
      661 acccgtttcg acgtggatgc cgcggcgggc gacgccatgg acgcacgcgc catggtgggc
      721 gacagcagcg cctggaccaa tctgagcact ttccgcggtc acggtggcaa gctgatcttc
      781 ctgcatggcg tcagcgatcc gtggttctcg gcgcaggaga gcgtgcggta ctacgaactg
      841 cttggccgcg acaataccga ggttccgctc gcggaatgga gccgcctgtt cctcgtgccc
      901 ggcatgggcc attgcggcgg aggctcgcgc acgctcgatc agttcgacat gctgagcgcc
      961 gtcgtcgact gggtggaatc cggccgcgcg ccggagcgcg tgatcgcgac cggcaaatca
     1021 atgccgggcg agagccggcc gctgtgcccc tatccgcagc acgcgcacta cgccggcagt
     1081 ggtgatgcgc acgaagcggc taactacatt tgccggcagt gaaccgctat tcggccgact
     1141 gcagatattc cctttcgcgg tattcgaagt aatcgaaatc cgcgggatgc gccatgcccg
     1201 acatgtcctg gcaggctacg cccacgaacg ccccggtgaa attggccgag cccggccccg
     1261 tgcactcatc ggacagcacg ctggcatcaa acaactcagg caggaatttc caatctccac
     1321 cgacgcgata cgcaaagcgc agccgctcgt aatcgacttc cacgcgcagt tcgatgcgac
     1381 ccgctggaat cggaatcgga gccgtgaaga aatccgactg cggcaggtcc ggcacgcatg
     1441 acatgacgcg cacgtgccgg ccatgctctt cgtcgtgcga cacgtgcaga taatggaact
     1501 tcgacgagtt gtagtaacac acgagccccg ccgcctgctg gaagtgcgcc ggctcgaagt
     1561 ccatcgccgt gacggcgctg taacaatgag cttgctgacg acgtgccacc agagactgcc
     1621 ggaaaacact gccgagggtt tcgcggccaa acaaacgcag gtgcccgggt cgttcgctca
     1681 ggctgaacaa ttcctcgggg tacggcgacc gcagccactg gaagtcgatg ggcagttgtt
     1741 tgctgtcgaa atcctcgcgc accgctgcgg cggcgttctt cgaatcgaac ggcgcggcgg
     1801 gcactcctga tggcgctggc gtctcgagct ctggcaatcc gctgccatcc tgcgtgcgta
     1861 gccagtcatc tttgccccag accatcttct ggatggcggt ctcgcggccc agcacgcatc
     1921 ggccgcgatt tggcagtggg cgcccgcata gatagacggc atatgtctcg cccgcctgcg
     1981 tgcccaccag atctccgtgg ccggcccgat gcagcggcgc atgcggtcgg tcccgcgcgg
     2041 tgatgatgta cttgtcggga tgcagctcgt acggcccggt cagcgagcgt gaacgcgcca
     2101 tcgtcatcgc gtggccccag cccgtgccgc cttcggcggt aatcaggtag tagtagccgt
     2161 tgcgcttgta gagatgcggc gcctcggtga agccgatcga agtgcctttg aaaatattct
     2221 tcggctcgcc gaccagcttg cgctgcgcgg gcgagtattc ctgcagcacg atgccggcga
     2281 agcggccgcg acccggccga tgatcccaga gctggttcac gaaatatttg cgcccgtcat
     2341 cgtcgtgaaa gagcgaggga tcgaagccgc tgctgttcac gtacagcgca tcggaccatt
     2401 cgccgtcgat ggtgggacat gtgaccaggt agttgtggaa atcgcgcagc gacgcgcccg
     2461 atgcgccgcc gaccgtcgtg cgcccgtaac gcttgacgtc cgtatagatc agccagaaga
     2521 gcccgtcgct gtagctgagg caaggcgccc agacaccgca tgagtcggga tcgccgagca
     2581 tgttgagctg gcttgcgcgc cgcagcggcc gggtcagcaa tcgccagttc accaaatcgc
     2641 gcgagtggtg aatctgcaca ccgggatacc actcgaaggt ggacgtcgcg atgtagtagt
     2701 cgtcgcccac ccgcaggatg gacggatcgg gattgaagcc cggcagaatt ggattgcgga
     2761 tcatgctgct gtcgcaaccg gtggggtcac gcgctcacgc acaacgctcc acagccagat
     2821 cgcggcgaga atatcgaaca cggccagcgc cacgaagaac gggtcgtaac cgacggtctt
     2881 caccagtccg ccgatcagca gggtgaacat cagcacgccg agatttccgc agaagccgcc
     2941 aatgccggcc accgtcgcca cttcattacg gcggaacagg tccgaagaca tggtgatgac
     3001 ggtgatcgac agcgtctggt gcgcaaagcc gcccacgcac agcaacgcga tcgcgacata
     3061 ggggctatcg accttgccga cgaacgccat gctggtcatc agtgccgcac cgaccgtgaa
     3121 ggcccagcgg cgcgcattga tgaggccgat gccgcgacgt tgcaaccaca gaacaatggt
     3181 cggccccgcg aggcagccga tgtccgccgt cacgaacggc agccaggcaa acaaggcgat
     3241 ctgcttgaga tcgaagccgc gctcagtggt gagatagaac ggaacccaga aggccagcgt
     3301 tccccaggtg ggatcggcga gcatgcgcgg aatcgcgatg ccccagacat tgcgctgctt
     3361 gagcaggttc aggatggacg tcttggtggc gcctgccgcc aggtgcgctt cctgaccgga
     3421 ctcgatgtag ttgcgctcct cggcggagat cttcggatga tccttcggcg agtaatagaa
     3481 cttgaaccac aacgccaccc acaccagcgc caaaccaccg gcgacgacga acgccagccg
     3541 ccagctatgg aacaggatgg cccaggcgac caacggcgcc gcgagcatcg agccgaacga
     3601 ggcgccgatg ttgtagaagc cgccggcgaa cccgcgctcc ttcgccggaa accactcggc
     3661 aaccgccttc atgccgccgg gctgcgccga cccttcggcg aaacccatca ggccgcgcaa
     3721 ggccgcgagc atctgccagt tgagcgcgag accgtggagc atcgtgatga ttgcccacgc
     3781 gatcgcaaac aggccgaagc caagcttgag gccgatgaga tccatcacgt agcccgcgaa
     3841 cggctgcagc atgatgccga tctggaagac gcccgtgatc caggagtatt cctgttcgcc
     3901 gatgccgagt tcgccggtga gcgtgggagc ggccaccccg agcgttgcac gggtgaggta
     3961 gttgaggatc gtgccgatcg tgatcaggct gatcatccac caacgcaacg catacagacg
     4021 ccggatcact ggcatgctcg tccccccacc gtgttaccgc taccctaaca cggtgagggg
     4081 ctgatttacg cgatcagtgc ttgtgccgag tcgagcaatc ccgatcccaa agcggcgcac
     4141 tttttgtaac cgatcggtgt gattcgacgc tcagaagttg atccggacgc cggcggtgaa
     4201 gaagcgtccc atgtcgtcgt agaaggccag cgtggggtag aacaatcccg gaagacccga
     4261 acccgacgga gccagcggag cacgttcatc cagaaggttg ttgacggtca ggaaagctgc
     4321 cagcgtgccg ccgctgacct cgaactcctt ggtgatcgtg gtatcgacga cgttgttggc
     4381 atcgagactc gagtccaggt aattctgggt attgccgttg aggtcattgt tgctgctctt
     4441 gagactggtg ctgccgagcc agcggttttg caacgcgacg ctccagctgc tgtttctgta
     4501 tgaaagaaac gtggtctgca tccatttggg ctgcacagcc cacgtgtaga acgtcgaagc
     4561 cggcgtggtg agcgtcgagt tgaccggctg atgggtgacc aggtggcgca aggcgaggtt
     4621 gccggcccca atatcccagt tgtagtcgag ctgcagatcg tagcccttga tcttctgcaa
     4681 cgccgcattg acgggcgcac ttcggacctc ggtcggcatg ttgacggccg gattgaagta
     4741 gtcgggatcg gacgggtcgg tgataggccg caccgccaga tcgcaaacgg gcgagctgta
     4801 ggtgggcgca ctggacaggc agatggcctg cacagaatcg ttcgcatagt tgaggttggt
     4861 gatcgcgtct tccagccggg tctcccagaa atcgaccgac aggccgaagc gcggcagggc
     4921 ggtcggcgtc agaacgaatc cgacggtaac cgtcgtggcc tcttctggcg tcaggagcgg
     4981 attgccacgc gaggcgagcc gcatgccctg ggcgctgccg cccgtgagcc ggtcgttgta
     5041 actgctcgag gtgactcccg agggctggta caagtcgttg aggtttggcg cgcggatatc
     5101 ggacgaatag gtgctgcgga tccgcaacga atcgactatc tgccagttca gcccgacctt
     5161 ccaggaatcg accgcatcga aggacgaata cttggtccag cgatacgcca gattggtcga
     5221 gaggtcctgg aagcccggca cctctttgag cagcggcacg ttaaattcca gcgcgccctc
     5281 gtagacgtga tcatcgacat cgacgggagc cacggtgttc tgaacccagc gaagcggtgc
     5341 ggcgccaccg gcggccagac acatgcgcag gcccgtgcag ttgacgaagt cggagggcag
     5401 gaagtcgctt tccaacacgt acgtggacca gcgcgcatcg accgacaggt tggcggtaat
     5461 ttcgccggcg ggaagaccaa agccccaaag gccgccgcca atggacgcgc cgacgttgtc
     5521 cagatcctgc gtcaggagcc atgacgtttt gactcgaagg tagtcatagg cgtccgccga
     5581 aatgccacct ggagcagtga tgttcgaggg aacgcagtcg ggataaagag aggcaaactg
     5641 cggctgtgtc gagacccagc acctgatcgt gccagcatca ttgaccgcat ccagcgaggc
     5701 caggtacttg gcgttgtcgg tgttgttggg attggtgacc gagagctcgc tggtgctgtg
     5761 gttgtagaac gcttcccagg tgaagccgcc aacctctccg gtgatgcccg tctccgcatt
     5821 ccactgcttt tgatcgccca gcgtgcgata catccggttc ggcccgccgg cgctgccatc
     5881 gacaggtagc ccgtcgacgt cgttgaagta gcgagggttg gcgaaaatgg ggatgttcgg
     5941 cggcggtggc ggcgtgctgc cggtttgcgg cgaggtcggc ggcgtggccg gcaggcaacg
     6001 ccagccggtc gcggcgggag ttccgcagac gatacccgat cccaggagcg cctgcgtggc
     6061 cggagtcaag tacggattgt tggcgaacag cgtgttgggc cggccgttgt ttgggctaac
     6121 tacccactgg atccagtcgg acgcattctc ggcttgcgcc caggacccct gcacatacca
     6181 gttggcgctc tcgcccacat ccaggctgaa gcgcgcgaag acatccttcg cgtccagttc
     6241 ggaacggaag gtgccgtact tgatgtaact gccgtcaccg cccgtttcta tgccaccgct
     6301 ggcgctcggc gtgccatgaa ccatcgggac caggccgcca gctgcatcga aggtgtagtt
     6361 gttgtatgcg cacgcggtgc cgcactgcac gttgccgttc ggaccactgt tgaagacgcg
     6421 cgaatacgga gtgaattcaa acggtctgcc gccgctcgcc acggtacggc cgttgccggt
     6481 ctgcaaccag gattgcccat cttcggcata gggacgctcg tgctgcggga tcatgtcctg
     6541 cttgcgtgcg cgggcggcca cttcgaagtg tccccgatca ctgaacagcg acgtgcccca
     6601 ggcggcgccc acctggtact ggtcaccatc gctgtacttc gacataccga aatcgccctt
     6661 cagcgaaagc ccggtgaaat tcttgtccaa catgtagttg accacgccgg cgacggcatc
     6721 cgagccgtag atcgctgatg caccgccggt cacgatgtcg acattcttga ccagcatctg
     6781 cggcagtacg tcgatgttga ccgagccgtc ctggttactc ggcgcgacgc ggtggccgtc
     6841 cagcagcacc agcgtgcgcg aggggccaaa gttacgcagc gacagcgtat tgccgccgtt
     6901 attggtgctg ccgttgccct ggttacgcgg tgtgcgtccg ccgatgatgc tgggcagctt
     6961 gttcaacgcg tcggccgtgt cgctcggcgt ggtaagcgcc atatcggcga cgtcgaccga
     7021 ggtgatcggc gtgggtgact ttaggacatc ggtgatggtg cgcgatcccg tcaccgttat
     7081 gacgcccata tcttcttcta caggctgttg ctcgggcgtt tgtgcccagg cggaattgcc
     7141 attgagtgct gctgaaacac caagcgcgac caaggacgcg cggaaatatg cacgtgaagt
     7201 catcgtcatc cccccttctt tgatgtcgcg acgctatcag catgttttta ctaacgcaat
     7261 tcacttaccg gattcgtatg t
//
LOCUS       DASQJS010000260         4923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_777609, whole genome shotgun sequence.
ACCESSION   DASQJS010000260 DASQJS010000000
VERSION     DASQJS010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777609"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VEW08_10475"
     CDS             <1..659
                     /locus_tag="VEW08_10475"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYJ41209.1"
                     /translation="AWRTLNTFPNATFNLATGNVGVMAATNEEVKAKEYPILGWVFAA
                     VILMCLLTFRSLLGTVLVFLPLALVSILVYAEMAIVGIGLKVNTLPMVALGAGIGVDY
                     GIYLWSRMQEYLHAGDTVHQAFEKTMRVTGASIIFTGITLAIGVVTWVFSPLQFQADI
                     GIMLTFMFFVNMLGAIILLPALAAWMVKAVPGGESGESRSTASSPQPPADSPLSPPGT
                     "
     gene            659..2098
                     /locus_tag="VEW08_10480"
     CDS             659..2098
                     /locus_tag="VEW08_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HYJ41210.1"
                     /translation="MTPEEFRRAGHELIDWVADYRERVARSEFPVMSRNVPGALRAAL
                     PAIPPQAPEHFAAIRRDLDELILPACTHWQDPRFHGYFPSNGLPAAVLGDLVSAGLGQ
                     LGLNWQASPALTELEEVCLDWWRQMLGLSARWSGVIQDTASTATFVALVCARERASNH
                     AATRGGLQGLASPLVVYVSAQAHSSVEKAALLAGFGRENLRMIPVDDNFDMRADALAT
                     AIATDRAAGREPAAIVATCGTTATTAFDPIAEIVQIARAQELWLHVDAAMAGSAMILP
                     ECRILWDGVEGADSLVVNPHKWLGVSMDCSTYYVRDPQFLVRVMSTNPSYLQTAADGT
                     AKNYRDWGLPLGRRMRALKLWFVIREQGVSGLQARISRDLAHAQWLAGEISATSPWKI
                     LAPVRLQTLVVRHEPPGFDVAAIDVHTRAWAQAINQSGKAFLTPAVINDRWAVRVSIG
                     AATTSREHVVDLWRLMRETAQAIQEGAQR"
     gene            2095..3042
                     /locus_tag="VEW08_10485"
     CDS             2095..3042
                     /locus_tag="VEW08_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ41211.1"
                     /translation="MKKTGIAIGLIVAAVSASAQQPVAPQTPPPPMPAPADVKIETIS
                     VAPGIYMLMGRGGNVGLTVGVDGAAIIDDQFADMAPKIRAAVTMLSEQPVQFVINTHL
                     HGDHTGGNDAFGKAGAVIIAHENVRKFLGSDQVNPSTNAPIPARAREALPVITFADTA
                     TLHFNDDDLQFMHLPNAHTATDIVVRFRKANVLHMGDCFTGGFPFIDGNTGGTLDGLI
                     RAHEKVLATVDDNTKIIRGHGPLGNKAELQAYHDMLVVVRDRVAKLVRAGKSADDVIA
                     ARPTRDYEEKYGGASFNAAQWLGRAYVDQKIALEKKKKK"
     gene            3273..3764
                     /locus_tag="VEW08_10490"
     CDS             3273..3764
                     /locus_tag="VEW08_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41212.1"
                     /translation="MKDSSNTAKKLLDLPYAPEAEQYLDELERSGQFRVGLEAEVEDA
                     EAGDASAGNDYGWGRGSGSVSIQDWWTSGRVALRVGAGIAPGEVTLARVNDSLVMRTQ
                     DGFDRLVLEGYLGLIPDASTLRIIFNDGHEWSGAELLARIGSWLTESESTQGEPGPSA
                     PNH"
     gene            complement(3773..4528)
                     /gene="tfpZ"
                     /locus_tag="VEW08_10495"
     CDS             complement(3773..4528)
                     /gene="tfpZ"
                     /locus_tag="VEW08_10495"
                     /inference="COORDINATES: protein motif:HMM:NF041437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfpX/TfpZ family type IV pilin accessory
                     protein"
                     /protein_id="HYJ41213.1"
                     /translation="MIVWREKFIATAIHFLVTLGLAACAAALIFLVWFPDPFATMIGG
                     TELFMLVVGCDVALGPLISLVIYNSRKSRRKLIIDYTIVGIVQIAALVYGVSIVAGTR
                     PAYVAYSIDRLEVVTAREFTDAELAAVRKPEYATLPFTGPRLVAIEVPPTDRNEALFS
                     GLNGNDIYLRPKFFVRYEAQVESIRGHARPIALLMKRKPASRSLLEAAMRDVEIPAER
                     VRWLPVHHSKGFWTALIDINDGKPIAYMDLDPY"
     gene            complement(4540..>4923)
                     /gene="arfB"
                     /locus_tag="VEW08_10500"
     CDS             complement(4540..>4923)
                     /gene="arfB"
                     /locus_tag="VEW08_10500"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HYJ41214.1"
                     /translation="DLEVAFIRSAGPGGQNVNKVASAVQLRFSVARNTTLRDDVKARL
                     RALAGQRLTDADEILIQARESRSQESNRRDAEERLLDLVRRALVVPKKRHATKPTRAS
                     KERRLDGKARSQKNKRLRGKVKFDD"
BASE COUNT          881 a         1576 c         1594 g          872 t
ORIGIN      
        1 aggcctggcg cacgctcaac accttcccga atgccacctt caacctcgcc accggcaatg
       61 tcggcgtcat ggcggccacg aacgaagaag tgaaggccaa ggaatatccc atcctgggct
      121 gggttttcgc cgccgtgatc ctcatgtgcc tgctcacgtt ccgcagcttg ttaggcacgg
      181 tgctggtgtt cctgccgctc gcgctcgtgt ccatcctggt gtacgcggag atggccatcg
      241 tcggcatcgg cctcaaggtc aacacgctgc ccatggtggc gctcggcgcc ggcatcggcg
      301 tggactacgg catctacctg tggagccgca tgcaggaata cctgcacgcc ggggacacgg
      361 tgcaccaggc gttcgagaag accatgcgcg tgaccggcgc gagcatcatc ttcaccggca
      421 tcacgctcgc catcggcgtg gtcacctggg tgttctcgcc gctgcagttc caggcggata
      481 tcggcatcat gctcacgttc atgttcttcg tgaacatgct tggcgccatc atcctgttgc
      541 ccgcgctcgc cgcgtggatg gtgaaggcgg tgccgggtgg ggaaagtggg gagtcccgat
      601 cgacggcgtc gtcgccgcaa ccaccagccg actccccact ttccccaccc ggcacctaat
      661 gactcccgaa gagttccgcc gcgcggggca tgagctcata gactgggtgg cggactatcg
      721 cgagcgcgtt gcgcggtccg aatttccggt gatgtcgcgc aatgttcccg gtgcattgcg
      781 cgcggcattg ccagccattc cgcctcaggc acccgagcat ttcgccgcca tccgccgcga
      841 tctcgatgag ctgatcctgc cggcctgcac tcattggcag gatccgcgtt ttcacggcta
      901 cttcccgtca aatggtttgc cggcggccgt gctcggcgat ctcgtgagcg cgggcctggg
      961 gcagctcggg ctcaactggc aggcaagtcc ggcgctgacc gagctcgaag aggtgtgcct
     1021 cgactggtgg cgccagatgc tcgggttgtc cgcgcgctgg tcgggcgtga ttcaggacac
     1081 cgcctccacg gccacgttcg tggcgctggt ctgtgcgcgt gagcgcgcct ccaaccacgc
     1141 cgccacgcgg ggcgggctgc agggcctggc gagcccgctc gtggtgtacg tatcggcaca
     1201 ggcccatagc tcggtggaaa aggcggcact gctcgcgggc ttcggtcgcg agaatctgcg
     1261 catgatccct gtcgacgaca acttcgacat gcgtgctgac gcgctcgcca cggccatcgc
     1321 gacggatcgc gccgccgggc gcgaaccggc cgcgatcgtg gcgacctgcg gcacgactgc
     1381 gaccaccgcg ttcgacccca ttgccgagat cgttcagatc gcgcgcgcgc aggagttgtg
     1441 gctgcacgtc gatgcggcca tggcgggctc ggccatgatc ctgcccgagt gccggatcct
     1501 gtgggatggc gtggaaggcg ccgactcact ggtcgtgaac ccgcacaagt ggcttggcgt
     1561 gtccatggac tgctctacgt actacgtgcg cgacccgcag ttcctcgtgc gcgtcatgtc
     1621 gaccaacccg agttacctgc agaccgcagc cgatggcacg gcaaaaaact accgagattg
     1681 gggattgcca ctcggccgcc gcatgcgggc gctcaagctg tggttcgtga tccgcgagca
     1741 gggcgtgagc gggctgcagg cgcgcatcag ccgcgatctc gcgcacgcgc aatggctggc
     1801 gggagaaatc agcgcaacgt cgccgtggaa gatcctcgcc ccggttcggt tgcagactct
     1861 ggtcgtgcgc cacgaacctc cggggttcga tgtcgcggcc atcgacgtgc acacccgtgc
     1921 ctgggcccag gccatcaacc aatccggtaa ggcattcctt actccggcgg tcatcaatga
     1981 caggtgggcc gtacgcgttt cgataggagc ggcgaccacc tcgcgtgagc acgtggtcga
     2041 tctctggcgg ctcatgcgtg agactgcaca agccattcag gaaggagcac agcgatgaag
     2101 aaaacgggaa ttgcgatagg gttgatcgtg gcagcagtta gcgcgagcgc gcagcagccc
     2161 gtggctccgc agacgccacc gccgcccatg cccgcgccag cggacgtgaa gatcgaaacc
     2221 atcagcgtgg cgcccggcat ctacatgctc atgggacgcg gtggcaacgt ggggctcacg
     2281 gtgggcgtcg acggcgccgc catcatcgac gatcagttcg ccgacatggc gcccaagatc
     2341 cgtgccgccg tcacgatgct ctcggaacag ccggtgcaat tcgtgatcaa cacgcacctg
     2401 cacggggatc acaccggcgg caacgacgcg ttcggcaagg ccggcgccgt catcatcgcg
     2461 cacgaaaacg tgcgcaagtt cctcgggtcg gatcaggtga atccttccac caacgcgcct
     2521 atcccggcac gcgcgcgcga agcgttgccc gtgatcacgt tcgctgacac ggcgacgctg
     2581 cacttcaatg acgacgacct gcagttcatg catctgccga acgcgcacac cgcgacggat
     2641 atcgtggtgc gattcaggaa ggccaacgtg ctgcacatgg gcgactgctt caccggcgga
     2701 tttccgttca tcgacggcaa caccggcggc acgctcgatg gcctgatccg cgcgcacgaa
     2761 aaggtgctcg cgacggtcga cgacaacacg aagatcatcc gcggccacgg accgctcggc
     2821 aacaaggcgg agctgcaggc gtatcacgac atgctcgtgg tggtgcgcga tcgcgtggcg
     2881 aagctggtca gggccggcaa gagcgccgac gacgtgatcg cggcgcggcc gacccgcgat
     2941 tacgaggaaa aatatggtgg cgccagtttc aatgcggcgc agtggttagg ccgcgcctac
     3001 gtcgaccaga agatcgcgct cgagaagaaa aagaagaagt aggcaggcgg agcgtgcgcg
     3061 ccgctgcagg agcggcggcg cacccctcgc gacgcacctt ccgggaatcc cctatcgctt
     3121 cgcgaagttg cgccgaatca gtgcaacata acgcgccgca tcacgggctt ttcgcccaac
     3181 tacgcggtgg aacgccccat cggcctatag acagcgccat gcctgctggt aaaagtttct
     3241 ctcgcaatgg tgcgatacgc cctgtggaag agatgaaaga ctccagcaat accgcaaaaa
     3301 agttgttaga cctgccgtac gctcccgagg cggaacagta cctcgatgag ctcgagcgga
     3361 gcgggcagtt ccgcgtcggc ctcgaagcgg aggtcgagga tgccgaagcg ggcgacgcgt
     3421 cggccggcaa cgactacggc tggggccgcg gctccggcag cgtctcgatt caggactggt
     3481 ggacttccgg gcgcgttgcg ttgcgcgtcg gggcaggaat cgcaccgggc gaagtgacgc
     3541 tcgcgcgcgt caacgattca ctggtgatgc gcacccagga cggcttcgac cggctcgtgc
     3601 tcgagggtta cctcggcctg attcccgatg cctcgaccct gcgcatcatc ttcaatgacg
     3661 gccatgaatg gtcgggcgcc gagctgttgg cgcgaatcgg aagctggctc accgagagtg
     3721 aatccacgca gggtgagccg gggccttcgg cgcctaacca ctagccgctg tctcaatacg
     3781 gatcgaggtc catgtaagcg atgggtttgc cgtcgttgat gtcgatcaat gcggtccaga
     3841 accccttgct atggtgaacg ggcagccagc ggacgcgctc cgcaggtatt tccacatcgc
     3901 gcatggcagc ttcgagcagc gacctgctgg cgggctttcg cttcattagc aaggcgatcg
     3961 gcctggcgtg cccgcggatg ctctcgacct gcgcttcgta acgaacgaaa aacttcggcc
     4021 gcaggtagat atcgttgcca tttaagcccg agaacaacgc ttcgtttctg tccgtcggcg
     4081 gcacctcgat agcgaccagg cgcggtccgg tgaacggcag cgtcgcgtat tccggtttgc
     4141 gcacggccgc gagctcggcg tccgtgaact cgcgcgccgt gacgacttcc agccggtcga
     4201 tgctgtaagc cacgtacgcc gggcgcgtgc ccgcgacgat ggaaacgccg tagaccagcg
     4261 cggcgatctg cacgatgccg acgatcgtgt agtcaatgat gagcttgcga cgcgatttgc
     4321 gactgttgta aatgaccagg gagatgagcg gcccgagcgc gacgtcgcaa cccacgacca
     4381 gcatgaacag ctcggtgccg ccgatcatgg tcgcgaaggg gtcgggaaac cagaccagga
     4441 aaatcagcgc tgcagcgcac gccgcaaggc ccagggtcac caggaagtgg atggccgttg
     4501 cgatgaattt ctcgcgccac acgatcatgt gttcctccgt cagtcgtcga atttgacctt
     4561 gccacgtagg cgcttatttt tctgcgagcg cgcctttccg tccagtcgac gctctttcga
     4621 ggcgcgtgtg ggtttggtcg catggcgctt cttggggact accaatgcgc gacggacgag
     4681 atctaggagc cgttcttccg cgtcgcgtcg attgctttcc tggctgcgcg actcgcgcgc
     4741 ctggatgagg atctcgtccg cgtcggtgag tcgctggccg gcgagggcgc gcaatcgcgc
     4801 cttcacgtca tcgcgcagcg tcgtattacg tgccaccgaa aatcgcagct gtacggccga
     4861 cgccaccttg ttgacgttct gcccgcccgg cccggcagag cggatgaacg ccacttccag
     4921 atc
//
LOCUS       DASQJS010000261         3658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_154112, whole genome shotgun sequence.
ACCESSION   DASQJS010000261 DASQJS010000000
VERSION     DASQJS010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154112"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1061
                     /gene="panC"
                     /locus_tag="VEW08_10505"
     CDS             210..1061
                     /gene="panC"
                     /locus_tag="VEW08_10505"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HYJ41215.1"
                     /translation="MSTVTTIAQVRAQVAEWRAARDRVVFVPTMGNLHAGHVSLIEAA
                     RRHGKRFVASIFVNPMQFGPNEDFAHYPRTPTQDATMLADAGCDLMFMPEVGEIYPNG
                     ADQATRVEVPGISGILCGEFRPGHFEGVATVVAKLFNIVDADVAIFGEKDFQQLTVIR
                     RMVADLCLRVEIVGAPTVREPDGLAMSSRNQYLDEAQRKLAPTIYRQLQQAVEALRSG
                     VRDYARIETAGRTALDGAGFRTDYFSVRDAKTLAPAKPDTKYFVVLTASRLGKARLID
                     NVQYTAG"
     gene            1065..1385
                     /locus_tag="VEW08_10510"
     CDS             1065..1385
                     /locus_tag="VEW08_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41216.1"
                     /translation="MGASSRRSCPQCGVDSVQLSLANVRRRRRVTCHSCGTKLEIVVP
                     TALYTLVTLSAVILGSMLVPVLLMSIFEKKWTAIALAVALLFALIFGSNELLNRRATV
                     QRLD"
     gene            complement(1382..2482)
                     /gene="queG"
                     /locus_tag="VEW08_10515"
     CDS             complement(1382..2482)
                     /gene="queG"
                     /locus_tag="VEW08_10515"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HYJ41217.1"
                     /translation="MTPPNESPVADPGALLELKNDLLALARERGFDALGVSDVQLSVD
                     AAHLDRWLGAGKHGEMGYMSKHGSRRTHPEELVPGTVRVLSARMNYWPSDARDATEAL
                     ADPMLGYISRYALGRDYHKVLRNALQSLADDIVERVGPMGYRVFVDSGPVLEKALARN
                     AGLGWIGKHTNLIARDAGSYFFIGEILTDLPLPVDEPASAHCGTCSACIPICPTGAIT
                     APNQLDARRCISYLTIELHGAIPVELRRMMGNRIYGCDDCQLVCPWNKFARAAAHPDF
                     KTRHRLDSSRLEELFAWTEAEFLDRTEGSAIRRIGYERWLRNIAIALGNAPATPANTA
                     ALTARADDPSALVREHVAWARAEHTTRQAAGS"
     gene            2510..>3658
                     /locus_tag="VEW08_10520"
     CDS             2510..>3658
                     /locus_tag="VEW08_10520"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HYJ41218.1"
                     /translation="MNRPTAIYSAAQVRALDAWEIEKRRVPAFTLMTRAAEGALKILR
                     ARWPQAKRVAVVCGAGNNGGDGYVLARLARLAGLEALVLAAAPPDKLGGDARRAMEEW
                     LATGGSAHPFAADALSGSDVIVDGLLGIGFAGALRAETLAVIRAINSAKRPVLALDIP
                     SGVNADSGAVSEAAVRAEVTVSFVALKSGLFLGAGPEHAGVVLLDDLGVVAPLTPQFT
                     PLMRRIDESELASSLPRRARESHKGAHGRVLVIGGGSGMPGALRLAGEAALRVGAGLV
                     TVAGAPENLVAVTATRPELIYLPLVSANLDDAIRSCDVLAIGPGLGTGDWARRLWAQV
                     RSAGIPVVVDADALNLLAANPGKLPQNWIITPHPGEAARLLGVDTRAVQ"
BASE COUNT          592 a         1181 c         1259 g          626 t
ORIGIN      
        1 cgacggtcag attctcgtgt tgtacgacgt gctcgacatc acctcgggcc gcaagccgcg
       61 cttcgtgaag aatttcatgg aaggcgccac atcgaatgtc gacgcgctca agcgctacgt
      121 cgaggcggtg aagaagaaaa cctatcccgc acccgagcac gaattccatg cttgaggcgc
      181 gccctgccga attgaagaag ggatcgcgga tgtcgaccgt gaccaccatt gcccaggtgc
      241 gcgcgcaggt ggcggaatgg cgcgccgcac gtgatcgcgt ggtgttcgtg ccgaccatgg
      301 gcaacctgca cgccggccac gtcagcctca tcgaggctgc gcgaaggcac ggaaaacgtt
      361 tcgtcgcgag catcttcgtg aacccgatgc agttcgggcc gaacgaagac ttcgcgcact
      421 atccgcgcac gcccacgcag gacgcgacca tgctcgccga cgccggctgc gatctcatgt
      481 tcatgcccga agttggcgaa atctatccga acggcgcgga ccaggcgacg cgcgtggaag
      541 tgccgggcat ctccggcatc ctgtgcggag aattccgtcc cgggcatttc gaaggcgtgg
      601 ccaccgtcgt cgccaagctg ttcaacatcg tcgatgccga cgtggcgatc ttcggcgaga
      661 aggatttcca gcagctcacc gtgatccgcc gcatggtcgc ggacctgtgc ctgcgcgtgg
      721 agatcgtcgg cgcgcccacg gtgcgcgaac ccgacgggct cgcgatgagc tcgcgcaacc
      781 agtacctgga cgaggcacag cgcaagctgg cgcccaccat ctaccgtcaa ttgcagcagg
      841 ccgtcgaggc gctgcggtcg ggcgtgcgtg actatgctcg catcgagacg gccggacgca
      901 cggcgctcga tggcgcgggc tttcgcaccg actacttcag cgtgcgcgac gcgaagacgc
      961 tggcaccggc gaagcccgac acgaaatact ttgtcgtctt gacggcgtcg cggctgggca
     1021 aggcccggct catcgacaac gtccagtaca ccgcgggttg acgcatgggc gcctcgtcgc
     1081 gccgaagttg tccccagtgc ggagtcgatt cggtccagct gtcgctggcc aacgtgcgcc
     1141 gccggcgccg ggtcacgtgc cattcctgcg gcacgaagct ggaaatcgtc gttccaaccg
     1201 cgctttacac cctcgtgacg ctgtctgccg tcattctcgg ttcgatgctc gtgccggttc
     1261 tcctcatgtc gatattcgag aagaagtgga ctgcgatcgc gctggccgtt gcgctgctgt
     1321 tcgcgctcat cttcgggtca aatgagttgt tgaatcgccg cgccaccgtg cagcgcctgg
     1381 actaactgcc tgcggcctgg cgcgtcgtgt gttcggcgcg ggcccatgcc acgtgttcac
     1441 gcaccagcgc cgaaggatca tcggcgcgcg cggtcagtgc cgcggtgttc gcgggagtag
     1501 ccggcgcatt gcccagcgcg atcgcgatgt tgcgcagcca gcgttcatag ccgatacgcc
     1561 ggattgccga gccttcagtg cgatcgagaa actccgcttc ggtccaggcg aacaactctt
     1621 ccagtcgcga agagtccagc cggtgccgcg tcttgaaatc cggatgcgcg gcggcgcgcg
     1681 cgaatttgtt ccacggacag acaagctggc agtcgtcgca gccatagatg cggttgccca
     1741 tcatgcggcg caactccacg ggtatcgcgc cgtgtaattc gatggtgagg taggagatgc
     1801 agcgccgcgc gtcgagctgg ttgggcgcgg tgatggcgcc cgtgggacag atcgggatgc
     1861 acgcgctgca ggtgccgcag tgagcgctcg cgggttcatc gacgggcagc ggcaggtcgg
     1921 tgaggatctc gccgatgaaa aaatacgagc cggcgtcgcg cgcaatgaga ttggtgtgtt
     1981 tgcctatcca gccgagcccg gcgttgcgcg ccagggcttt ttcgagcacg ggtccggaat
     2041 cgacgaacac gcggtaaccc atcggcccca cgcgctcgac gatgtcgtcg gccaggctct
     2101 gcagggcgtt tcgcaacacc ttgtgatagt cgcgaccgag cgcgtaacgc gagatgtacc
     2161 ccagcatggg atcggccagc gcctcggtgg catcgcgcgc gtccgatggc cagtagttca
     2221 tgcgcgccga caggacgcgc accgtgcccg gcaccagctc ttcggggtgc gtgcggcgcg
     2281 agccatgttt ggacatgtag cccatctcgc cgtgtttgcc cgcgccgagc catcggtcga
     2341 ggtgcgcggc atccactgag agctgcacgt cgctgacgcc gagcgcgtcg aaaccacgct
     2401 cccgggccaa agccagcagg tcgttcttga gctccagcag cgcgcctggg tcggcaactg
     2461 gggattcatt gggcggagtc atggtgtcgt ggccagtata attcgcgcca tgaaccggcc
     2521 gaccgccatc tactccgccg cgcaggtgcg cgcgctggac gcctgggaaa tcgagaaacg
     2581 ccgcgtcccg gccttcacac tgatgacccg ggcagcggaa ggcgcgctga aaatcctgcg
     2641 cgcccgttgg ccgcaggcga aacgcgtggc ggtggtctgt ggcgcgggca acaacggcgg
     2701 cgacggctac gtgctcgcgc gcctcgcgcg cctggccggc ctcgaggcgt tggtgctggc
     2761 ggcggcgccg cccgacaagc tgggtggcga tgcgcgccgc gcgatggagg aatggctggc
     2821 caccgggggt agcgcacatc ccttcgccgc cgatgcgctg tcaggcagcg atgtcatcgt
     2881 cgacggtttg ttgggcatcg ggttcgcggg cgcattgcgc gcggaaacgc tggccgtgat
     2941 ccgcgcgatc aactcggcga agcgtccggt gctggcgctc gacattccct ccggagtgaa
     3001 cgccgacagc ggcgcggtgt cggaggccgc ggtgcgcgcc gaagtcacgg tgagtttcgt
     3061 ggcgctgaag tccgggctgt tcctcggtgc gggtccggag catgcgggcg tcgtgctgct
     3121 cgacgacctg ggcgtggtgg ctcctctcac tccgcaattc acgccgctca tgcggcgaat
     3181 cgatgaaagc gaactcgcgt cgagcctgcc gcggcgggcg cgcgagtcgc acaagggggc
     3241 ccacggccgg gtgctggtca tcggcggcgg ctccggcatg ccaggcgcgc tgcggctggc
     3301 gggagaggcg gcgttgcgcg tgggcgccgg gctcgtcacg gttgccggcg cgccggagaa
     3361 cctcgtggcc gtcaccgcca cgcgtccgga actcatctat ctacctctcg taagcgccaa
     3421 cctggacgat gccatccgat cctgcgacgt gctggccatc gggcccgggc tcggcacggg
     3481 cgactgggcg cgacgcctgt gggcgcaggt gcgttcggcc ggcatccccg tggtggtgga
     3541 tgccgatgca ctgaatctgc tggcggcgaa tcccggcaaa cttccacaga actggatcat
     3601 cacgccacat cctggcgaag ccgcgaggtt gttaggcgtt gacacccgcg ccgtgcag
//
LOCUS       DASQJS010000262        16152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2662894, whole genome shotgun sequence.
ACCESSION   DASQJS010000262 DASQJS010000000
VERSION     DASQJS010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16152
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2662894"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..2416
                     /locus_tag="VEW08_10525"
     CDS             269..2416
                     /locus_tag="VEW08_10525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HYJ41219.1"
                     /translation="MRFKTALLSLACLWGTLGIQTSVSAETDTAKDPKTLQTIRVEAT
                     AEATSTAAKLPLSLRETPQSVTIVTRERLDDQNLLSLQDVLESTPGVYTYAWDSERVV
                     FTSRGFVIDNLMYDGVPAEGNFSTGSIDETIDTALYDRIEIVRGATGLMTGAGSPAAS
                     INLFRKHADNKDLATSLGFTAGSWSEYRLDADVSTPLTSDGSVRARFVGVYQDTDSFQ
                     NLYTKEKTVLYGIVDADLSENSRLSFGVDFQDNKPKSNTWGSFPLYLADGTPANWARS
                     VTTSTDWAFWNRKTTSAFAELQHSFDNGWSLRSTLSWRRFEEDLALFYVYGFPDPETG
                     EGLEPFAYRADGEITSLALDVHASGAVNLFGREHELVVGYNGSKADNTGNEYANGELP
                     PVGNFFEWDGSYPEPVFDPTPILLTDVDSKQNGLYVAGRFVLADPLKAIAGVRYSTWE
                     TDHFYLYDSPDETFHDEYRKVIPYAGLIFDISEHYSVFASFTEIFKPQLALDENAKYL
                     DPVDGNSVEFGIKGEHFDARLNTALTLFETKQDNVAVPAFDEDGAPIPVPGHAGVQAS
                     LAVDGTRTRGFEFEASGALGESWNASLGWTRYLIEDAEGIAIRTFVPRTMVRGFTTWR
                     PAALEKLTLGAGINWQSDSYTLVGIPDGLATLREGDVTQLSLMARYQFTPKISVQLNG
                     SNLLDEDYYVLDDYDNTRYGEPLAVSASLNWQF"
     gene            2520..2786
                     /locus_tag="VEW08_10530"
     CDS             2520..2786
                     /locus_tag="VEW08_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41220.1"
                     /translation="MGIAAPHNLRRPDAGIQRPFGLRITLKAGDPFKKLLGADWNKTH
                     WYGTAAERDAALVEMARKHEYSRPGDKPALKFDKVEKLHESRGL"
     gene            complement(2862..5111)
                     /locus_tag="VEW08_10535"
     CDS             complement(2862..5111)
                     /locus_tag="VEW08_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYJ41221.1"
                     /translation="MTNKARKSALFAVFCLGVCASVPAQEAAKPAEASRPRIGLVLSG
                     GGARGAAHIGVLKVLEENHVPVDAIAGTSMGAVVGGLYASGLSAAEVERVMTSVDWQD
                     AFRDSPPRSSLNFRRKLEDQNYLVKFPLGLKGWRFRLPRGLVQGQKLTQMLRGLTLPV
                     AQIQNFDDLAIPFRAVATDIVTGDRVILDHGDLTTAMRASLSAPGVFSPVESEGRMLV
                     DGGLSSNLPIDVAREMGVDILIVVDCGFPLLERGKLDSVATVSNQMLAILIRHNTAEQ
                     RKTLKATDVVIDPALGDFSSLDFNQHAKAMRIGEQAARGQTERLAALSIPADEFQRVV
                     DARAARRSGAPEIKFLRVEEGSERYAGAIEALFGDQVDKPVSVPKLERRVGELYGQGN
                     LEILDYRLVQSDPERGEEPGYGLSLNSRRNSWGPNYLRFGLQLQNDFEGNSSFNAAAR
                     TTMAEITKYGGEWVVDLQVGETPRVSTEVYLPVGYRSRWFVMPHAQFQIRTLPLITAD
                     EDILAEYRVRTTDFGLDFGRELGNYGEIRVGAGRTVGSARVRVGDPLLPPSDFDTNNF
                     FSEFRYDTVDNVNFPRNGASFQLGWRGEREGGGDSQDADLLVFDQLFARSWGRHTMML
                     WTSGGTRLDNDADVLRSYFSLGGFLNMSGITPETLVGPHYAIARGIYYRQVGRRGQGF
                     LNVPVYVGASLETGNVWTSRRDISLESARTNGSLFIGLDTLLGPVYFATGFDEGGGSA
                     YYLFLGRTF"
     gene            5161..7269
                     /locus_tag="VEW08_10540"
     CDS             5161..7269
                     /locus_tag="VEW08_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ41222.1"
                     /translation="MRIPLTLVALLPLAAQAADEPVAASGEMEESYAEDIVVTATRDR
                     RVAADTPASISRIDYETIAAIGSKHQADVLNRNAGVYIQRGSGAESLAAIRSPVLAGA
                     GACGAFLVAEDSLPIRPTGFCNNNEMFEVNYEQARQIEVLRGPGSSMFGASAVHGVIN
                     TITPAVRQLPEFEMGLEGGSDSFKRVTAAGSWYPSPDGPSFGAYGVATRAPGWRDESG
                     VDEAKLNLLGDIWTGGGQLRLRASGTVLNQETAGFIQGYNSYKDEDIARSNPNPEAFR
                     DASSARVSAQYENDDAFGEGNPLQVAAIYRRSRMDFLQHFLIGKPLEHNAQTSYMLSG
                     TAAFSSADFMTTRVTVDAELAGSELTEYQPGPATDGAPPANAIRPAGYHYDYTVDSST
                     VGATLAFDFRIGPDFTVTAAVRADQTNYDYDNHMIDGNTRDNGTSCAAAGCLYSRPAD
                     RTDRFENFSPALAVAWRPGSTLLYANLSTGFRPPETTELYRLQRQQSEADLDSENLDS
                     VEVGWKFRGDFLSLDGAFFEMKKDDVILRETNGFNVGNGATHHRGFEYEARLDRERWS
                     LSLNGTFARHEYAFSRAVEGGETIVDGNDVDTAPRSLHALAVDARLGESDEWQLGLDV
                     NYVGKYFLDAANTATYPGHTVANARIAWSPAKTWRAALRVDNLFDTRYADRADFAFGN
                     YRYFPARGRAVFLSVDYARN"
     gene            7280..8035
                     /locus_tag="VEW08_10545"
     CDS             7280..8035
                     /locus_tag="VEW08_10545"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ41223.1"
                     /translation="MAWIIYIVSGLLSLGFIVHCIKTGGNTIWVYVLVVLMSFPFVGS
                     MVYFGAEILPGLLKARTTQRAMRGIRTTLDPEGNLRKYENEMQITGNVASRQRYADEL
                     VKLGRASEALAIYQTCLTGVFVDDPKLLLGYAQAQFEAGDPAAARRTLDELIQKNPQF
                     KSADGHLLYARAVEAEGDMAKALSEYATLAEYYPGAEAGVRYARLLNKSDQRPLAQQT
                     LKALLDRAKFAPAHYRKAQREWLDEAHRELQNR"
     gene            complement(8036..8230)
                     /locus_tag="VEW08_10550"
     CDS             complement(8036..8230)
                     /locus_tag="VEW08_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41224.1"
                     /translation="MAAKKKGARHMREWSAGDMKQLRQFAKQKVSARVAAQKLGRSPG
                     AVRYKAMMEGVSFRSINRKK"
     gene            complement(8362..9192)
                     /locus_tag="VEW08_10555"
     CDS             complement(8362..9192)
                     /locus_tag="VEW08_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HYJ41225.1"
                     /translation="MSQKTVPRRDIPDRLRIRPGSRTKLLDSDADRCFGWEKEKAMAA
                     TAENLLRLEELQYKMYADKRFAMLTVLQAIDGGGKDSTIRRVFGAFNPQGCTVTAFKA
                     PSAEDLRHDYLWRIHRHAPPRGEIAVFNRSHYEDVLIVRVNDLVPEDVWSRRYEQINE
                     FERLLDACNTRVVKIFLQISKEEQRVRFQERLTDRRKNWKFDTGDLEKRQQWTAYTRA
                     FEAMFARCSTEHAPWYVVPANRKWFRDFAVSQLLIYEFEQLPLRFPAPNFDPKNIKLV
                     "
     gene            complement(9189..10280)
                     /gene="aguA"
                     /locus_tag="VEW08_10560"
     CDS             complement(9189..10280)
                     /gene="aguA"
                     /locus_tag="VEW08_10560"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF010070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HYJ41226.1"
                     /translation="MAKTLDSTPLADGFSMPAEFAPHAGCWMLWPERPDNWRLGALPA
                     QQAFANVATAIARFEPVTVGASAGHFEFARAQLPAHVRVVEMSHDDAWMRDVGPTFVT
                     NDKGVRRGVDWRFNAWGGLSGGLYFPWDQDDLVANKVLEIEACDRYRAHIVNEGGAIH
                     VDGEGTALVTEQVLLNANRNPGIAREEMERHLKAYLGVQAVIWLGEGVVDDETGGHID
                     NLACFLRPGSVALHWTDNKRDPQFAVSQDALEKLSAARDARGRRLTVIKLPAPGPLRY
                     RPEEVLGVQVRDTVRGRIAGMRLAGSYVNFYIANGGIVMPLLDPRTDKAAAAKLKRAF
                     PGRKVVGVPAREILLGGGNIHCITQQVPR"
     gene            10387..11700
                     /gene="icd"
                     /locus_tag="VEW08_10565"
     CDS             10387..11700
                     /gene="icd"
                     /locus_tag="VEW08_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HYJ41227.1"
                     /translation="MATAPTSPKVTPPAGGAKITIQNGKLTVPDQPIIPFIIGDGTGP
                     DIWHASVRVMDAAVAKAYGGKKKIHWLEIYAGEKSFKAFNNWLPDETVEACREYLVSI
                     KGPLTTPIGGGIRSLNVALRQMLDLYVCLRPVRWFKGVPSPVKAPEKVDMVIFRENTE
                     DIYAGIEFEHGSEENKKFKELFKAAFPKAYAKIRFPDTSGIGFKPVSQEGTERLFKSA
                     LEYAIAFKRRSVTIVHKGNIQKFTEGAFRNWAYALAESGTFKDKFYTWEEWGRTKAKS
                     GEKAANDEQAAALKSGKILVKDAIADIALQQVLTRPDEFDVIATTNLNGDYLSDALAA
                     QVGGIGIAPGANINYITGHAVFEATHGTAPKYADLDKVNPGSVILSGEMMLRYMGWTE
                     AADAIISAMDKTIAQKTVTYDFARLMEGAKEVKCSEFGDALIRNM"
     gene            complement(11749..12627)
                     /locus_tag="VEW08_10570"
     CDS             complement(11749..12627)
                     /locus_tag="VEW08_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYJ41228.1"
                     /translation="MPRRLRIHVPSGVYHVTLRGNHRQPIFHSDCDRNLLNVIVARAL
                     EKFETRLHAFCWMTNHVHMVLQAGPEPISRPMHDIAAEFARAMQLKLETTGHFFERRF
                     HAVLVDTDSYLLELLRYVHRNPVTAGIAASVDAYRWTSHHNYRGVRHDNWVTTNLALD
                     MFAPERARAIAAYCRFVEVEDAQPWEPLQALAPRVAVIGNEEFVARAMRSREPGKTRQ
                     SLEELLQEACRRFEVGRDSLTSPVRNAYVARVRAWIAYQAGERGIATLAAVARALGRT
                     EGALRYAIRAYPNDIK"
     gene            complement(12718..13899)
                     /locus_tag="VEW08_10575"
     CDS             complement(12718..13899)
                     /locus_tag="VEW08_10575"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYJ41229.1"
                     /translation="MKKPTLVNHPPDVSPPAGNLPVIAPIYQSVKYEFESVDDTLRML
                     RGEAPGYFYMRASNPTTRQLELTLAQLQGRDDCLASASGVNAIAQTLIGLTKQGDHIL
                     CFVETYGPTRNIIRRLLSRFGVEHTMLSIEDMAGIENVLATRPTRLVIFESPTNPITK
                     VADIERLVELAHRHGALAVMDNTFAGFHQHGEYEVDLFVHSLTKYASGTGDVMGGAVI
                     GRRELIEGLRADFAVLGALLDPHAAFLVQRGLKTYFVRYQEQTRSAQAIAEHLAAHPA
                     VSKVHYPGLPGHPHIALARKQMREAGTVVSFDLKEGGEAARRFCEGLKLFALAASLGS
                     TESLVMAPQLMGTRDFTPEQARQSGIAPGTVRLSIGLEDLGDLIADIDRALGGAGHQK
                     S"
     gene            complement(13919..14695)
                     /locus_tag="VEW08_10580"
     CDS             complement(13919..14695)
                     /locus_tag="VEW08_10580"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HYJ41230.1"
                     /translation="MSFRVSIAQQPLVWQDAAANRAHFAKALAPLAGTTDLVVLPEMF
                     PTGFTMKPEEHAEAADGPTRAWLQEQATALDAAVGGSVAVNDNGRYYNRFMIATPGGL
                     TYWYDKRHLFRMGGEHRHYSAGVHALIVEWRGARLCPLVCYDLRFPVWSRRRPELDYD
                     IVIFTANWPAARRFAWTSLLRARAIENQAYCVGVNRVGDDGAGIAHSGDSVVLDFTGQ
                     PLLELNDHAQVITVPLDLPGLRAWRDKFPAHLDADAFSLE"
     gene            complement(14692..15888)
                     /locus_tag="VEW08_10585"
     CDS             complement(14692..15888)
                     /locus_tag="VEW08_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HYJ41231.1"
                     /translation="MSFPEGFQGKLPDVGTTIFTVMSRRAAELGAVNVGQGFPDYSID
                     PRLAACVTDAVAAGFNQYAPMEGNLALRAAIAAKIAAGGGRAVDPETEITVTCGGAES
                     IYSAIQAMVGAGDEAIVFDPAYDAYDPAIRLAGGRCIHIPLEAPHFRYDWNRVRSALS
                     DRTRLILINNPHNPACTVASAADLEALAQLTRDRPIAVLADEVYEHVQYDGRVHQSVI
                     NHAELRSRSFAVYSFGKTLHITGWRVGYCVAPPSLTRELRKVHQFNAFSTPAPLQQAI
                     ALYLQRHPDAWRELVPFFSAKRELLRRGLAGSGLVLPTAEGTYFQLADYSSLEGSLAE
                     LNDVEFTERLINDAGVAVIPLSPFYREPPAGMRIVRLCVAKRDETLTEAAARISAYTR
                     AKREKP"
     gene            complement(15942..>16152)
                     /locus_tag="VEW08_10590"
     CDS             complement(15942..>16152)
                     /locus_tag="VEW08_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41232.1"
                     /translation="APWTPADLKKMRALAKDKMSARLAAKELGRSTGAVKYKAMVEGI
                     RFRFIDQPAGVQLKLARKAKRAKKK"
BASE COUNT         3054 a         5124 c         5064 g         2910 t
ORIGIN      
        1 cggatgctat tggtggcgcc tgcacgcgcg caaatgcctg tctgaattga ggctttttac
       61 cgggcaaagg ttgaagggaa gccacgataa accagcaaaa gcccgtttcg cccctgttct
      121 tctcaaataa gaataattat catctggccc tcaaatcggc ccttcggggc ctccgggacc
      181 agccagtcaa tcctgatgcc cttcgggctc aaggccgcca gccatcccct tcttatctac
      241 gtggcgcaaa gccaccgaag gattggccat gcggttcaaa actgccctgc tgtcgttggc
      301 gtgcctctgg ggaacgctcg gcatccagac aagtgtctct gctgaaacgg atacggcgaa
      361 ggatcccaaa acgttgcaga ccattcgcgt cgaagccacg gccgaggcga ccagtaccgc
      421 agccaaacta ccgctttcgc tgcgcgaaac accgcagtcg gtcacgatcg tcacgcgcga
      481 acgcctcgac gaccagaacc tgctgtcgct gcaggacgtg ctcgaaagca cgcccggcgt
      541 gtacacctac gcctgggaca gcgaacgcgt ggtcttcacc tcgcgcggct tcgtcatcga
      601 caatctgatg tacgacggcg tgccggcgga aggcaatttc agcacagggt cgatcgacga
      661 aacgatcgac accgcgctct acgatcgaat cgaaatcgtc cgtggcgcca cgggcctcat
      721 gaccggcgcc gggagcccgg ccgcatccat caacctcttt cgcaagcacg cggacaacaa
      781 ggatctggcg accagcctgg ggttcacggc cggttcctgg agcgaatacc ggctcgatgc
      841 cgatgtatct acgccgctca ccagcgatgg cagcgtgcgc gcgcgtttcg tcggcgtgta
      901 ccaggacacc gattcgttcc agaacctgta cacgaaggaa aagacggtgc tctacggcat
      961 cgtcgatgcc gacctttcgg aaaacagcag gctctccttc ggcgtcgact tccaggacaa
     1021 caaaccgaag tccaacacct ggggctcgtt tccgctgtac ctcgccgacg gaacgccggc
     1081 aaactgggca cgctcggtga ctacgtcgac ggactgggcc ttctggaacc gcaagaccac
     1141 gtccgcattc gctgaactcc agcactcctt cgacaacggc tggtctttgc gcagcacgct
     1201 gagctggcgc cggttcgaag aagacctcgc cctgttctac gtctacggtt tcccggatcc
     1261 cgaaaccggc gaagggctcg aaccgttcgc gtatcgcgcc gatggcgaga tcacctcact
     1321 cgcgctcgac gtgcatgcct cgggagccgt gaacctgttc ggccgcgaac acgagctggt
     1381 cgtgggctac aacggctcga aagcggacaa cacgggcaat gaatacgcga acggcgaact
     1441 gccgccggtc ggcaatttct tcgagtggga cggttcatat ccggagccgg tcttcgaccc
     1501 gaccccgatc ctgctcaccg acgtcgactc gaaacagaac ggcctgtatg tcgctggccg
     1561 cttcgtgctg gccgatccgc tcaaggccat cgccggtgtt cgctacagca cctgggagac
     1621 cgatcacttc tatctatacg attcgcccga cgaaaccttc cacgacgaat acaggaaggt
     1681 gattccctat gccggcctga tcttcgacat cagcgagcac tattcggtgt tcgcgagctt
     1741 cacggaaatc ttcaagcccc aactcgcgct cgacgagaac gccaaatacc tcgatcccgt
     1801 cgacggcaac agcgtggaat tcggcatcaa gggcgagcac ttcgatgcgc gcctgaacac
     1861 ggcgctcacg ctgttcgaaa cgaaacagga caacgtggcc gtcccggcgt tcgacgaaga
     1921 tggcgcgccc attccggtgc cgggccacgc cggcgtacag gcatcattag ccgtcgacgg
     1981 tacgcgtacg cgcggcttcg agttcgaagc ctcgggcgct cttggcgaga gctggaatgc
     2041 gtcgctcggc tggactcgtt atctgatcga agacgcggag ggcatcgcga tccgcacgtt
     2101 cgtgccgcgc acgatggtgc gcgggttcac cacttggcgg cccgcggcgc tggagaagct
     2161 gacgctgggc gccggcatca actggcagtc tgatagctac accctggtgg ggatacccga
     2221 tggcctggcc actctgcgcg aaggcgacgt gacgcagctc agcctcatgg cgcgctacca
     2281 gttcacgccg aagatttcgg tgcagttgaa cggcagcaac ctgctcgacg aggactacta
     2341 tgtgctcgac gactacgaca acacgcgtta tggcgagccg ctggcggtgt cggcgtcgct
     2401 taactggcag ttctgagccc cgcccggcct gatcgcgatg cgtcgcgggc gagacttgct
     2461 ctcgcccgtg gcgcttcgta acctagcgcc gcattcggca ttcgccggac ggagaattca
     2521 tgggtattgc ggctcctcac aacctgcgtc ggcccgacgc gggcattcag cgtcccttcg
     2581 gcctgcgcat cacgctgaag gccggcgatc cgttcaagaa gctgctcggc gcggactgga
     2641 acaagacgca ctggtacggc acggcggccg aacgagacgc ggcgctggtt gaaatggcgc
     2701 gcaagcacga atactcacgc cccggcgaca agccggcgct gaagttcgac aaggtcgaga
     2761 agctgcacga atcgcgcggt ctctgagcgc ccgtatttct tcttttcgca tttgatgtac
     2821 gccgccgctt cggcggtgtt ttccgacgtc agcgtcagcc gtcaaaaagt cctgccaagg
     2881 aacaggtagt acgccgagcc gccgccctca tcgaagcctg tggcgaagta caccggacct
     2941 aacaaggtgt ccaggccgat gaacaggctg ccattggtcc tggcgctctc gagggagatg
     3001 tcgcgccgcg aggtccacac gttgccagtc tcgagcgagg cgccgacgta cacgggcacg
     3061 ttcaggaacc cctgccctcg ccgcccgacc tgccggtagt agatgccgcg cgcaatcgcg
     3121 taatgcggcc cgaccagggt ttcgggcgtg atgcccgaca tgttgagaaa tccacccagc
     3181 gaaaagtatg aacgcaggac gtcggcgtcg ttgtcgagcc gggtgccgcc gctggtccag
     3241 agcatcatgg tgtggcgccc ccaggagcgc gcaaagagct ggtcgaacac cagcaggtcc
     3301 gcgtcctgcg aatcccctcc accctcccgc tcacccctcc agccgagctg gaacgaggct
     3361 ccgttgcgcg ggaagttcac gttgtcgaca gtgtcgtaac ggaattcgct gaagaagttg
     3421 ttggtgtcga agtcggaagg cggcagcaac ggatcgccaa cgcgcacgcg cgccgaaccg
     3481 acggttcgcc cggcgcccac gcggatttca ccgtagttgc cgagttcacg accaaagtcg
     3541 aggccaaagt cggtggttcg tacgcggtat tcggccagga tgtcttcgtc ggcggtgatc
     3601 agcggcagcg tacgaatctg gaactgcgcg tgcggcatca cgaaccagcg tgagcgatat
     3661 cccacgggca gatacacctc ggtggacacg cgcggggtct cgcccacctg cagatccacc
     3721 acccactcgc caccgtactt ggtgatctcg gccatcgtcg tccgggcggc ggcattgaac
     3781 gaggagttgc cctcgaagtc gttctgcagc tgcagcccga agcgcagata gttaggaccc
     3841 caggaattgc ggcgactgtt cagcgacagg ccatatcccg gctcttcgcc ccgttcgggg
     3901 tccgattgca ccaggcgata gtcgaggatt tccaggttgc cctggccata caactcaccg
     3961 acgcggcgtt cgagcttggg cacgctgaca ggtttgtcca cctggtcgcc gaacagcgcc
     4021 tcgatggcgc cggcataacg ctccgaaccc tcttcgacgc gcaggaactt gatttcgggc
     4081 gcaccgctgc ggcgggcagc tcgcgcatcg acgacgcgct ggaattcgtc cgcgggaatc
     4141 gacaacgcgg cgaggcgctc tgtctggccg cgggcggcct gctcgccgat gcgcatcgcc
     4201 ttcgcgtgct ggttgaagtc gagcgaagag aagtcgccga gcgccgggtc gatcaccacg
     4261 tccgtggctt tcagcgtctt gcgctgctcg gccgtgttgt gccggatcag gatggcgagc
     4321 atctggttgg atacggtcgc cacggaatcg agcttgccgc gttcgagcag cggaaaaccg
     4381 caatcgacga cgatgaggat gtccacgccc atctcgcgcg ccacgtcgat gggcaggttc
     4441 gacgacagcc cgccgtcgac cagcatgcgt ccttcggatt ccacgggcga gaacacgccg
     4501 ggtgcggaca ggctcgcgcg catcgccgta gttaggtcgc catgatcgag gatgacccgg
     4561 tcgccggtga cgatgtcggt ggccacggcg cggaacggta tggcgaggtc gtcgaagttc
     4621 tggatctgcg ccaccggcag cgtgagacca cgcagcatct gcgtgagctt ctgtccctgg
     4681 accaggccac gtggcaggcg gaagcgccaa cccttcaggc ccaacggaaa cttcaccagg
     4741 tagttctggt cctcgagttt gcggcggaaa ttgagcgagc tgcgcggcgg gctgtcacga
     4801 aaggcgtcct gccagtcgac cgaagtcatg acacgttcga cttccgccgc cgagagtccg
     4861 gacgcgtaca ggccgccaac gaccgcgccc atgctggtgc cagcaatggc atcgactggc
     4921 acgtggtttt cctcgagcac cttgagaacg ccaatgtgcg ccgcgccacg cgcaccgcca
     4981 cctgacaaca ccaatccgat ccgggggcgg gaggcttccg ccggcttcgc tgcctcctgg
     5041 gcgggtacgg atgcgcagac cccgaggcag aacacggcaa acagcgctga ttttcttgct
     5101 ttgttggtca ttcgtggcgt ggcaaggata gtctttcacg gggataatcg cccagccaac
     5161 atgcgcatac ctctcaccct ggtggccttg ctgccgctcg ctgcccaagc ggctgacgag
     5221 cccgttgccg cgagcgggga gatggaagag tcctatgcgg aggacatcgt cgtaaccgcc
     5281 acgcgcgaca gacgtgtcgc cgccgataca ccggccagca tttcccgcat cgactacgag
     5341 accatcgcgg ccatcggctc caaacatcag gcggatgtgc tcaatcgcaa tgccggcgtt
     5401 tatatccagc gcggcagcgg cgctgaaagt ctcgccgcca ttcggtcacc ggtgctcgcg
     5461 ggtgccggcg cctgcggcgc attcctggta gcggaagaca gtctgccgat ccgcccgacc
     5521 ggcttctgca acaacaacga gatgttcgag gtcaactacg agcaggctcg ccagatcgaa
     5581 gtgctgcgcg gcccggggtc gtcgatgttt ggcgccagcg ccgtccacgg agtcatcaat
     5641 acgatcacgc ccgccgtgcg ccagctgcct gaattcgaaa tgggccttga gggcggatcc
     5701 gattccttca aacgagtgac cgccgccggg agttggtatc ccagccccga cggaccttcc
     5761 ttcggcgctt acggcgtcgc gactcgcgct ccgggctggc gcgacgagtc gggcgtcgac
     5821 gaggccaagc tcaatctgct gggcgatatc tggactggcg gcgggcaact gcggctgcgc
     5881 gcctcgggca ccgttctcaa ccaggaaacc gccggattca tccagggcta taacagctac
     5941 aaggacgagg acatcgcgcg cagcaatccg aatcccgaag cgtttcgcga tgcctcgagt
     6001 gcgcgcgtgt cggcgcagta cgaaaatgac gatgcgttcg gtgagggaaa cccgctgcag
     6061 gtcgccgcca tttaccgccg ctcgcgcatg gatttccttc agcatttcct gatcggcaag
     6121 ccgctcgaac acaatgcgca gaccagctac atgctgagcg gtacggcggc gttcagcagc
     6181 gcggatttca tgacgactcg cgtcacggtc gacgccgaac tggcaggctc cgagctcacc
     6241 gagtaccagc cgggcccggc caccgacggc gcgccaccgg ccaatgccat tcgtccggcg
     6301 ggatatcact acgactacac cgtggattcc agcacggtgg gcgccacgct cgcgttcgat
     6361 ttccgcattg gccccgactt caccgtgacc gcggccgtgc gcgccgacca gactaactac
     6421 gattacgaca atcacatgat cgatggcaac acgcgcgaca acggcacgtc ctgcgcggca
     6481 gccggctgcc tctattcgcg gcccgccgat cgcaccgatc gcttcgagaa tttctcgcca
     6541 gcgctcgcgg tggcgtggag accgggtagc acgctcttat atgccaatct gtccaccggc
     6601 ttccgccctc cggagactac cgaactgtac cgcctgcagc gacagcagtc ggaggccgat
     6661 ctcgacagcg agaacctcga ttcagtcgag gttggctgga agtttcgcgg cgactttctc
     6721 tcgctcgacg gcgcgttctt cgaaatgaag aaggacgacg tgatcctgcg cgagaccaat
     6781 ggcttcaacg tgggtaacgg cgccacccat catcgcggct tcgaatacga ggcgcgactc
     6841 gaccgcgagc gctggagcct gtcgctcaac ggcacttttg cgcggcacga atacgcgttc
     6901 tcgcgcgccg tggagggtgg cgagaccatc gtcgacggca acgatgtcga caccgcgccg
     6961 cgcagcctgc atgcgctggc ggtcgacgcc aggctcggcg aatcagatga gtggcaactg
     7021 gggctggatg tcaactacgt cggcaagtac ttcctggatg ccgccaacac cgcgacctac
     7081 cccgggcata ctgtggccaa cgcgcgcatc gcctggagcc cggcaaaaac ctggcgggct
     7141 gcactgcgag tcgacaacct tttcgacacg cgttatgccg atcgcgcaga cttcgctttc
     7201 ggcaactacc gctatttccc cgcgcgcggt cgcgcggtgt tcctgtccgt ggattacgcg
     7261 cgcaactaag aggaatgacg tggcctggat catctacatc gtctcgggat tgctttcgtt
     7321 gggattcatc gtccattgca tcaagaccgg cgggaatacc atctgggtgt atgtgctcgt
     7381 ggtactcatg tcctttccct tcgtgggatc catggtctac ttcggtgctg aaatccttcc
     7441 cgggttgttg aaagcgcgca ccacccagcg tgccatgcgc ggcatacgca ccacgctcga
     7501 tccggagggc aacctccgca agtacgaaaa tgaaatgcag atcacgggca acgtcgcctc
     7561 gcgccagcgg tatgcggacg agctcgtgaa gctgggccgg gccagcgaag cgctggctat
     7621 ctatcagact tgtctgacag gcgttttcgt cgatgatccc aagctgctgc tgggttacgc
     7681 gcaggcgcag ttcgaagcgg gagatccggc cgcggcgcgc cggacactcg atgaactcat
     7741 ccagaaaaat ccgcagttca agtccgcgga cggccacctg ctgtacgcgc gcgcggtcga
     7801 agccgagggc gacatggcga aggcattgtc ggagtacgca acgctggcgg agtactaccc
     7861 gggagccgag gcgggcgtgc gttatgcgcg gctactgaac aagtcggatc agcggccact
     7921 ggcccagcag acgctcaaag cgctgctgga ccgggccaaa ttcgctccag ctcactatcg
     7981 caaggcccag cgagagtggc tggacgaagc gcacagagag ctgcagaacc gctagttatt
     8041 tcttgcggtt gatggaacgg aacgagacgc cttccatcat ggccttgtag cgcactgcac
     8101 cgggtgagcg gcccagcttc tgcgcggcga ctcgcgccga cactttctgc ttggcgaact
     8161 ggcgcagctg cttcatgtcg cccgccgacc attcgcgcat gtgcctcgca ccttttttct
     8221 tggctgccat tccggagtcc tctcattcgt gtggtaggaa tttgagggcc gtagtctaac
     8281 aacaattacc cgggttggag tcaatcgtcg ccggatgcag acatgtaaga caagggcgcc
     8341 tatcaacctt acgccggccc ctcaaacaag cttgatgttc ttgggatcga agttaggagc
     8401 cggaaaacgc aacggcagct gctcaaactc gtaaataagc agctgtgaaa ccgcgaaatc
     8461 gcggaaccat ttgcgatttg cgggcactac gtaccaaggc gcgtgttcgg tggaacacct
     8521 ggcaaacatc gcctcgaatg cccgcgtgta ggcggtccac tgctgacgct tctccagatc
     8581 gccggtatca aacttccagt tcttgcgtct gtccgtcagg cgctcctgga agcggacccg
     8641 ctgctcttcc ttgctgatct gcaggaaaat ttttacaacc cgggtgttgc aggcatccag
     8701 cagacgctcg aattcgttga tctgttcgta acgccggctc cagacgtctt ctggaacgag
     8761 gtcattcacg cgcacgatga gcacgtcttc gtagtgtgag cggttgaata ccgcgatctc
     8821 gccccgcggc ggtgcatgcc tgtggatgcg ccacaaatag tcgtggcgca gatcttcggc
     8881 gcttggcgcc ttgaatgcgg taaccgtgca gccctgcgga ttgaaagcgc caaacacgcg
     8941 ccggatggtg ctgtccttgc caccgccgtc gatggcctgc agcaccgtca gcatggcgaa
     9001 gcgcttgtcc gcgtacatct tgtactgcag ctcttcgagc cggagcaggt tttcggcggt
     9061 cgcggccatc gccttttctt tttcccaacc gaagcagcga tcggcatcgg aatcaaggag
     9121 tttggttcgc gaacccggcc ggattcgcag ccggtcgggt atgtcgcgtc gcggcacggt
     9181 tttctggctc accttggcac ctgctgggta atgcagtgaa tatttccgcc acctaacagg
     9241 atctcgcgcg ccggcacgcc gaccaccttg cggccgggaa atgcacgctt gagcttcgcg
     9301 gccgccgcct tgtcagtgcg cggatccagc aatggcatga cgatgccgcc attggcgatg
     9361 tagaaattca cgtagctccc ggccagccgc atgcccgcaa tgcggccacg cacggtgtcg
     9421 cgcacttgca cgcccaggac ttcctccggc cggtagcgca acggcccggg agcaggaagc
     9481 ttgatcacgg tgagcctgcg gccacgtgca tcgcgtgcgg cggagagttt ctcgagcgca
     9541 tcctgcgata ccgcaaattg cggatcgcgc ttgttgtcgg tccagtgcaa tgccacagag
     9601 cccggtcgca ggaaacacgc gagattgtcg atgtggccac cagtttcatc gtctacgaca
     9661 ccctcgccca accagatgac agcctgtacg ccgagatagg ccttaaggtg ccgctccatc
     9721 tcttccctgg caatgccggg attgcgattc gcattcagca gcacctgttc ggtgaccagt
     9781 gcagttccct cgccatcgac gtgaatggcg ccgccttcat tgacgatatg agcacggtag
     9841 cgatcgcaag cttcgatctc gagcaccttg tttgcgacga gatcgtcctg gtcccaggga
     9901 aagtagagcc cgcccgagag tcctccccag gcgttgaaac gccagtcgac accacgccgg
     9961 acgcccttgt cattcgtcac gaacgtcgga cctacgtcgc gcatccatgc atcgtcgtgg
    10021 gacatctcga cgacgcgcac gtgcgccggg agctgcgccc gtgcgaattc gaagtgtccg
    10081 gccgacgccc ccaccgtgac cggctcgaac ctggcgattg ctgtcgccac gtttgcaaat
    10141 gcctgttgtg cgggcaatgc gccgaggcgc cagttgtcgg ggcgctccgg ccagagcatc
    10201 cagcagcctg catgcggcgc aaactcggcc ggcatggaaa agccgtcggc gagcggggtg
    10261 gaatcgagcg tttttgccat ggggcaggat tgtagcgggc cagggatgca acaaagcacg
    10321 ctgtgctagc atcgcggccc ttccgattcc cacccccccg ctaaaaggcg agagaaagta
    10381 catacgatgg ctaccgcgcc cacgagtccc aaagtcacgc cgccggcagg cggagcgaag
    10441 atcaccatcc agaacggcaa gctgacggtc cccgatcagc cgatcatccc gttcatcatt
    10501 ggtgacggca ccggtcccga catctggcat gccagcgtgc gcgtcatgga cgccgcggtg
    10561 gccaaggcct acggcggcaa gaagaagatc cactggctcg agatctacgc gggcgaaaaa
    10621 tccttcaagg cgttcaacaa ctggctgccc gacgagacgg tcgaggcgtg ccgcgaatac
    10681 ctggtgtcca tcaagggccc cctgaccacg cccattggcg gtggcattcg ctcgctgaac
    10741 gtcgcgctgc gacagatgtt agacctgtat gtgtgcctgc gcccggtacg ctggttcaag
    10801 ggtgtgccct cgcccgtcaa ggcgcccgag aaggtcgaca tggtgatctt ccgtgagaac
    10861 accgaagaca tttacgccgg catcgaattc gaacacggct ccgaagagaa caagaagttc
    10921 aaggagctgt tcaaggccgc tttcccgaag gcgtacgcca agatccgttt cccggacacc
    10981 tcgggcatcg gcttcaagcc cgtatcgcag gaagggacgg agcgcctgtt caagtcggcg
    11041 ctcgaatacg ccatcgcatt caagcgccgc agcgtcacca tcgtgcacaa gggcaacatc
    11101 cagaagttca ccgaaggcgc gttccgcaac tgggcctatg cgctggcgga gagcggcacc
    11161 ttcaaggaca agttctacac ctgggaagaa tggggccgca ccaaggcgaa gtccggcgaa
    11221 aaggccgcga acgacgagca ggctgctgcg ctcaagagcg gcaagatcct cgtgaaggac
    11281 gccatcgccg acatcgcgct gcagcaggtg ctgacgcgtc ccgatgagtt cgatgtcatc
    11341 gccacgacga acctgaacgg cgactatcta tccgatgcgc tggcggcgca ggtgggcggc
    11401 atcggcattg cgccgggcgc caacatcaac tacatcacgg gccacgcggt gttcgaagcc
    11461 acgcacggca cggcgccgaa gtacgcggat ctcgacaagg tgaatccggg ctcggtgatc
    11521 ctgtcgggcg agatgatgct gcgttacatg ggctggaccg aagcggccga tgccatcatt
    11581 tcggcgatgg acaagaccat cgcccagaag accgtcactt acgacttcgc gcgcctcatg
    11641 gaaggcgcca aggaagtgaa gtgcagcgag tttggcgacg cactgatccg gaatatgtaa
    11701 tgaggtggtg gtgccgggca ccaaaagccg tagttcgtcc ttgttccatc acttgatgtc
    11761 attgggataa gcacggatgg cgtagcgcag cgcgccttcc gtgcgaccca aggcccttgc
    11821 cactgcggca agcgtcgcga ttccgcgttc accggcctgg tacgcgatcc acgcgcgaac
    11881 tctcgcgacg tacgcattgc gcacgggcga ggtcagcgaa tcgcgcccca cttcaaaacg
    11941 ccggcaagcc tcctgcagca gttcctccaa cgattgccgc gtctttcctg gctctcgtga
    12001 ccgcatcgcg cgtgcaacga attcttcgtt gccgatcacc gcaactcgtg gcgccagcgc
    12061 ctgcaagggc tcccacggct gcgcatcctc gacctccacg aaccggcaat acgccgcgat
    12121 cgcccgcgcg cgttcagggg cgaacatgtc cagcgccagg ttcgtcgtga cccaattgtc
    12181 gtggcgaaca ccgcggtagt tgtgatgact cgtccagcga taggcgtcaa cgcttgcggc
    12241 aatgccggcc gtgacgggat tgcggtgcac gtagcgcagc agttccaaca ggtagctgtc
    12301 ggtgtccacc agcaccgcgt ggaagcggcg ctcgaagaag tggccggtag tctcgagctt
    12361 gagctgcatt gcgcgcgcga actcggcggc gatgtcgtgc atcggacgtg agatgggctc
    12421 aggacccgcc tgtagcacca tgtgcacgtg gttggtcatc cagcagaagg cgtgcagtcg
    12481 cgtctcgaac ttctcgagtg cgcgcgcaac aatgacattg agcaaattgc gatcgcagtc
    12541 ggaatggaaa atcggttggc gatgattccc ccgcaaggtg acgtggtaga cgccactggg
    12601 gacgtggata cgcagtcggc gcggcatcgc tgactcggct ctggtctgga aactgactcc
    12661 attgtgcggg cttcagcacg ctggaactaa gcccgaaatc gactgcgttt gctcgaatta
    12721 cgacttttgg tgcccggcac cacccagcgc ccggtcgatg tcggcgatga gatcgcccag
    12781 gtcttcgagg ccgatggaca gtcggacggt gcccggagcg attcctgact gacgcgcctg
    12841 ctcgggggtg aaatcgcgcg tgcccatgag ctgcggcgcc atcaccagag actcggtgga
    12901 cccgaggctg gcggcgagag caaacaactt gaggccctcg cagaagcggc gcgccgcttc
    12961 cccgccttct ttcaggtcga agctgaccac cgtgcccgcc tcgcgcatct gcttcctggc
    13021 gagcgctata tgcgggtggc ctggaagacc cgggtagtgc actttgctca ccgccggatg
    13081 tgcggccagg tgttcggcga tggcctgggc gctgcgcgtc tgttcctgat aacgcacgaa
    13141 gtaggtcttg agtccgcgct gtacgagaaa cgcggcatgc gggtcgagca gtgcgccgag
    13201 caccgcaaaa tccgcccgca gcccctcgat gagctcacgc cgtccgatga ccgcgccacc
    13261 catgacatca ccggtacccg atgcgtactt ggtgaggctg tgcacgaaca ggtcgacttc
    13321 gtattcgccg tgctggtgaa agccggcgaa ggtgttgtcc atgactgcga gcgcgccgtg
    13381 gcggtgcgcc agctcgacca gccgctcgat gtccgccacc ttggtgatgg gattggtagg
    13441 gctctcgaag atcaccagtc gcgtaggcct cgtggcgagc acgttttcga ttccggccat
    13501 gtcctcgatg gagagcatgg tgtgctcgac gccgaaccgg gacaggaggc gccggatgat
    13561 gttgcgcgtg ggcccgtagg tttccacgaa acacaggatg tggtcgccct gcttcgtcag
    13621 cccgatgagc gtttgcgcga tggcattgac gcccgaggcg ctggcgaggc agtcgtcgcg
    13681 gccctggagc tgagccagtg tcagctcgag ctggcgcgtg gtggggttgc tggcccgcat
    13741 gtagaaatag ccgggggctt cgccgcgcaa catgcgcaac gtatcgtcca ccgactcgaa
    13801 ttcatacttg acgctctggt agataggcgc gatgaccggc aggttgccag ccggcggcga
    13861 aacatcgggc gggtgattga cgagcgtcgg cttcttcatg agtccagcgg gatcttcttt
    13921 attccagtga aaaggcatcg gcatcgagat gcgcgggaaa cttgtcgcgc caggcgcgca
    13981 ggccgggcag atcgagcggc acggtgatga cctgcgcgtg gtcgttgagc tcgagcagcg
    14041 gctggccggt gaaatcgagc accacgctgt cgccggaatg cgcgatgccc gctccgtcat
    14101 cgccaacgcg gttcactcct acgcagtagg cctggttctc gatggcgcgc gcccgcagca
    14161 gcgaggtcca cgcgaagcgg cgcgccgcgg gccagttggc cgtaaagatg acgatgtcgt
    14221 aatcgagctc ggggcggcgc cggctccaca ccgggaagcg caggtcgtaa cacaccagcg
    14281 ggcacaggcg cgccccgcgc cactcgacga tgagcgcatg cacgccagcg ctgtagtggc
    14341 ggtgttcgcc gcccatgcgg aacaggtgcc gcttgtcgta ccagtacgtg aggccgccgg
    14401 gcgtcgcaat catgaagcgg ttgtagtagc ggccgttgtc gttcacggcg acgcttccgc
    14461 cgactgcggc gtcgagtgcc gtggcctgct cctgcaacca ggcgcgcgtc gggccgtccg
    14521 cggcttccgc atgctcctcg ggcttcatcg tgaacccagt ggggaacatc tcgggcaaca
    14581 cgacgaggtc cgtggtgccg gccagcgggg ccagggcctt ggcgaagtgc gcgcgattgg
    14641 ccgccgcgtc ctgccagacc agcggctgct gggcgatgct gactcgaaaa ctcatggctt
    14701 ttctctcttg gcgcgagtgt acgcgctgat gcgcgccgcg gcttcggtca gcgtttcatc
    14761 gcgcttggcc acacacagcc gcacgatgcg catgccggcg ggcggttcac ggtagaacgg
    14821 ggacagcggg atgacggcga cgccggcgtc attgatgagc cgctcggtga actccacgtc
    14881 gttcaattcg gcgagcgacc cctcgagcga tgaatagtcg gcgagttgga aataggtgcc
    14941 ctcggcggtg ggcaacacta ggccgctgcc cgcgagaccc cggcgcagca gctcgcgctt
    15001 ggcgctgaag aaaggcacca gctcccgcca ggcgtccggg tgccgctgta gatagagcgc
    15061 gatcgcctgc tggagcggcg ccggcgtgct gaaggcattg aactggtgca ccttgcgaag
    15121 ctcgcgcgtc agcgacggtg gcgccacgca gtaacccacg cgccaccccg ttatgtgcag
    15181 ggtcttgccg aaggaataca ccgcgaagct gcgtgatcgc agctccgcat gattgatcac
    15241 ggactggtga acacgtccgt catactgcac gtgttcgtag acttcatcgg cgagcacggc
    15301 gatcggccgg tcgcgagtca gctgcgccag cgcttcgaga tccgccgccg atgccacggt
    15361 gcaggccggg ttgtgcggat tgttgatcag gatgagccgg gtacgatcac tgagcgcgct
    15421 gcggacgcga ttccagtcat agcggaagtg cggcgcctcg agcggaatgt ggatacagcg
    15481 cccgcccgcg agccggatag ccgggtcgta ggcgtcgtag gccgggtcga agacgatggc
    15541 ctcgtcaccc gcccccacca tggcttgaat ggcggagtag atagattcgg cgccgccgca
    15601 ggtgaccgtg atctcggttt cgggatcgac ggctcgcccg ccgccggccg cgattttcgc
    15661 ggcaatggcg gcccggagcg caagattgcc ttccatgggc gcgtactggt tgaagccggc
    15721 ggcgacggcg tcggtgacgc aggcagccag cctgggatct atcgaatagt cagggaagcc
    15781 ctgaccaaca ttgaccgcgc ccagttccgc ggcgcggcgt gacatcacgg tgaagatcgt
    15841 ggtgccgacg tcgggcagtt tgccctggaa cccttccgga aaactcatcg gcttgaagaa
    15901 agccgccgcg cacccccgta cgcggcgtct tcgtctgacg cctacttctt cttggcgcgc
    15961 ttggccttac gggcgagctt cagctgaacg cctgcgggct gatcgatgaa acggaagcgg
    16021 ataccctcaa ccattgcctt gtacttgacc gctccggtgg aacggccgag ctccttcgcg
    16081 gcgagacgag cggacatctt gtccttcgcc aaggcacgca tcttcttcag atcggcgggc
    16141 gtccagggag ca
//
LOCUS       DASQJS010000263        14204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4235367, whole genome shotgun sequence.
ACCESSION   DASQJS010000263 DASQJS010000000
VERSION     DASQJS010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14204
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4235367"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..844
                     /locus_tag="VEW08_10595"
     CDS             53..844
                     /locus_tag="VEW08_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003300391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ41233.1"
                     /translation="MDLKLGGKLALVSGSTAGIGYSIAEALAAEGARVIVNGRSQAGV
                     DAALASLRKTTNKDALGFAGDLSEAAVAEALVKAHPGIEILVNNLGIFEPKAFEEIPD
                     ADWRRFFDVNVLSGARLARLVLPAMRRANWGRIIFISSESGVQIPAEMVHYGMTKAAQ
                     IAVARGIAEAVAGTGITVNSVLPGPTKSRGVGDFVDALAKEQGKSGAEFEKEFFEHVR
                     PTSLLKRFSTPQEVASLVAYVASPLSSATTGAALRVDGGTVKSAF"
     gene            845..1351
                     /locus_tag="VEW08_10600"
     CDS             845..1351
                     /locus_tag="VEW08_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41234.1"
                     /translation="MIVKRSKVVKIPGNEEWSGYKDDLDARHAHGLWFGKSLDDMQPL
                     FSGGRSIERGSELLFMPRRAFQFYVFAFAQYVMSEAAIGDPDGASCFLNYLTAREKRD
                     PGSVAAIFSRLEQTIDFVAASQARFDASHDIYGDFTEKAEDFRKLIGATHSTHDPEDQ
                     MLDPTDDA"
     gene            1351..1797
                     /locus_tag="VEW08_10605"
     CDS             1351..1797
                     /locus_tag="VEW08_10605"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HYJ41235.1"
                     /translation="MTTEYEAMLEVAIAEAKRGLDEGGIPIGAALYHNDGRLLGCGHN
                     RRVQEGDPSVHGETDAFRKAGRQRRYRDTIMVTTLAPCWYCSGLVVQFRIGTVVVGES
                     RTFQGGIDWLRERGVKVVDLGSYECEKLLGDYIAANPEVWNEDIGE"
     gene            complement(1808..2209)
                     /locus_tag="VEW08_10610"
     CDS             complement(1808..2209)
                     /locus_tag="VEW08_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41236.1"
                     /translation="MRFTSANSRNQLRERQRRDRATAQVMRVMYPQFASLKLEFDFSE
                     SDPDISAPAPHLLVMHPPSQAYFVFPCPYADCDGEFDLTSAVAELARSREPHAHGSTK
                     CGGQRVRDKNGRTPCQLTLEYTIEPAAEPVA"
     gene            complement(2206..2412)
                     /locus_tag="VEW08_10615"
     CDS             complement(2206..2412)
                     /locus_tag="VEW08_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41237.1"
                     /translation="MAKTNYNQAKRQKENARKARQQEKLAKKLARVSGEPEAGEVGSV
                     EPGAVGDVAAPAADITPAAPKAVP"
     gene            complement(2405..2788)
                     /locus_tag="VEW08_10620"
     CDS             complement(2405..2788)
                     /locus_tag="VEW08_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41238.1"
                     /translation="MLRLNFRSIKGDAPTLTVATFFRFHADGTLRGPENYLVARCVDG
                     CWRLSGRMHRELDCEGPVKLRFSMGANDAPVMLGPFANVRTAAGMLYGDDSCLNIFVP
                     GRNPGTSAACHELTLLSAAGDRSFG"
     gene            complement(2820..3095)
                     /locus_tag="VEW08_10625"
     CDS             complement(2820..3095)
                     /locus_tag="VEW08_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HYJ41239.1"
                     /translation="MKLYVGNLPFSATEDSVRALFAEHGNVEKVALVNDRDTGQPRGF
                     GFVEMANADAQKAMQSLNGRDFGGRPLKVNEAQDKPRGGGGGGYNRR"
     gene            complement(3367..4194)
                     /locus_tag="VEW08_10630"
     CDS             complement(3367..4194)
                     /locus_tag="VEW08_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ41240.1"
                     /translation="MNAVLSRNAPRIDHRAVIARRHLPYASMDYAVAGNALQLVSEEL
                     CETVNLCQDRRVLDVATGNFAASLAAARRWCEVTATDHATDLAPRGRQRLEAESVGVR
                     IVDGDVDGLPFADQSFDAVLSCFGAMFAADQERAASEMIRVCRRGGQVGLTNWTPDGF
                     IGQLFRTVIRHSPPGPAAMPCDWGTRERIEDLFAAYGDIEVDGKRVAFRSRTPMDWVD
                     KLRTAYLPVHGIFASLDADRKRSLRSDLLELVAKFNRATDGTMVVDAAYLEVAITRR"
     gene            4267..6273
                     /locus_tag="VEW08_10635"
     CDS             4267..6273
                     /locus_tag="VEW08_10635"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ41241.1"
                     /translation="MESLEIRLLGPLQLARGGEVLKLPSSRKVRALLGYLVLASRPVT
                     RSQVCELLWDVPNDPRGELRWCLSKIRGLIDEKSRKRIIADGSTIRLDLSDCIVDALE
                     VTRAPEHGIKKLGVERQQALVAQFAGELLEGLEISRSPMFDAWITAERRRFRGIQTVL
                     LENLARALPHEAAAPYLDEWLKLSPFDRNAHELLLGALARHGRIADGEAHLASACKLF
                     EADGLDSAPLHELWRNTREKHAAPMQPAHAQPGPAQLVSASLTEIHRLAPDASAATRR
                     ASIAVMPFVDRSSEADKRGGAADALAYDVITRLAKLRSMFVIAQGTTFALRDRAIGPE
                     EAGRMLNVDYVVSGSVRRNGRKFTVSADLIETRTARIVWAEVFDEKVDDALDVLDEIG
                     NRIVASIAHEIEMVERNRAVLKPPNSLDAWEAHHRGLWHMYRFNKADNERARQFFSQA
                     VELDPTFARAYAGLSFAHFQNAFLGWKKPAPEIDRAFEAAGKSLMVDDRDPAAHWAMG
                     RAMWLRGGHDQAVTELERAVDLSPNYASAHYTLAFVHSQSGDPHAAISFSDHSRQLSP
                     FDPLLFAMLGSRAISLARLGQYEEASEWAIKAAARPNAHHHIMAIAALSLGLAGRIEE
                     ANEYKDRIRQRVPDYAMTDFLAAFGYSPEAEALFKQAGRKVGIT"
     gene            6370..6711
                     /locus_tag="VEW08_10640"
     CDS             6370..6711
                     /locus_tag="VEW08_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41242.1"
                     /translation="MKTIGCFIAITLLGLAPGAFAQVNIPNPSAPGNSPDSAVRLVAT
                     SELMVDRYISRWIRTHYPGWSADPHEFMEIGFERYAVVYITSPNNPGRRIYFRVMKNQ
                     NEDEGSGMPPL"
     gene            6846..7025
                     /locus_tag="VEW08_10645"
     CDS             6846..7025
                     /locus_tag="VEW08_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HYJ41243.1"
                     /translation="MSTDNPYAQYHVVKQGDTLSKIAEEYYGDKMLYPKIFEANKDVL
                     QDPNKIKPGQKLRIP"
     gene            7049..7651
                     /locus_tag="VEW08_10650"
     CDS             7049..7651
                     /locus_tag="VEW08_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41244.1"
                     /translation="MRFAKIFVALAATTLMIAGCASQKEPAEKAMAQAESSVAEFRAD
                     AEKYAADELAKVDQSITKLKNALANKDYNVVVKAVPSLNSTIASLKQSVAAKKADADA
                     MMAAATAEWTELSASVPQMVEAIQSRVDTLGKSRKLPANVDKATWETVKTDFETMKTE
                     WTDATTQFASGQADAAVRKAREVKAKGDAILGKLGMTPNA"
     gene            complement(7671..8798)
                     /locus_tag="VEW08_10655"
     CDS             complement(7671..8798)
                     /locus_tag="VEW08_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41245.1"
                     /translation="MNASGRHFRMVLLGIILAGALRPALSADLVVHEWGTITTIHAAD
                     GTPAGGLNSIDKADVLPDFVHRYEPETTRYKPERILGKLPNIPGRPDITMRLETPVIY
                     FHPPPNKAYTAPIDITVRFRGGVINEFYPDGAASVALDRERIADKMHEGVITTEWNGD
                     VLNNYVVGGLRWKGLRLHDTVVAPLTRSEIWLAPREVNSVSVFSPEAGEGEQYLFYRG
                     VAHLDALLATKTTRGSVELSTPANLAWLESAAVVPNVWLADVRADGIIAFRSQPAVTL
                     KKENAGKGLAKVKRFSGGDYTADGAAQLRSSLKKALIAQGLFADEAEAMLNTWKASYF
                     QKPGLRVFYIVPREWTDYFLPLEFSVPARVTRVIVGRIDLE"
     gene            complement(8912..9520)
                     /locus_tag="VEW08_10660"
     CDS             complement(8912..9520)
                     /locus_tag="VEW08_10660"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYJ41246.1"
                     /translation="MYRLVFATTLALLGLPAFATTYTLEPNYTQGVLRWDHLGFSYPA
                     AQFAQGTGTLDFDESDPTMASIKVTIPIASLNTGVPDLDEHLKSEDFFELAKYPTATF
                     ISTRVEKGMGTNRFKVTGNLTIRKVTRPVMLDVTLLKVGKNARTEVATIGFDAKGTLK
                     RSEFGLGAFVPQVSDEIQLLVTTQAAESKAYAAFLEAKKAKK"
     gene            9649..10518
                     /locus_tag="VEW08_10665"
     CDS             9649..10518
                     /locus_tag="VEW08_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HYJ41247.1"
                     /translation="MDRLTTIALQVGAWRIDPQLGQMSRGDQTVRLEARTLRLLMYLA
                     NRAGETVGIEELLDQVWSGVVVTQDSVYQAVTALRRLLGDDAKQPTYIVTVPRLGYRL
                     IAPVRAAVEEPASAILPPSLPPAPATRATQMRRSALLGVLVALAGLGFLAWRNSIAPD
                     AKSVAVMPFLDLTSQAMDEEYFADGLTEELIDRLSREPGFKVPAASASYFYKNKQLPP
                     SRIAQELGVVYLLEGSLRESGDTLRITARLTRAADGYVIWTSSYERSKSDRLAIQDEI
                     ALKVTAALRDSVR"
     gene            complement(10515..>14204)
                     /locus_tag="VEW08_10670"
     CDS             complement(10515..>14204)
                     /locus_tag="VEW08_10670"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HYJ41248.1"
                     /translation="DARSDPTIVNSGHVRERDIGIVIPGAPLEAVMSGEVWASVYDQL
                     AALISDHHTTLVFANTRRMVERVSRHLAERLGEEHVAAHHGSLSKETRFDAEQRLKAG
                     KLKVMVATASLELGIDIGDVELVCQLGTPRSISVFLQRVGRANHSVGGVPKGRIFPSS
                     RDELVDCTALLDSVRRTELDRLHIPEHPLDVLSQQIVAEVAAREYGEDELFALMRGAY
                     PYRNLERKDFDAVVRMLAEGIDTKRGRRGTYLHRDAVNQRLRARKGARLTAITCGGAI
                     PDNADYQVILEPAGVFVGTLNEDFAIESLAGDVFQLGNTSYKILRVEAGRVRVEDAKG
                     QPPSIPFWLGEAPARTDELSESVSRLRADIETLLPEVTAESLASAIDIVIERYHLPRP
                     AAHQMVEYLAAAKAVLGELPKRDTLVFERFFDESGGQQFIIHSPFGARVNRAFGLSLR
                     KKFCRTFNFELQAAATEDAIVLSLGETHSFDLASVARFLNSRTVEDTLIQAMLDSPMF
                     TARWRWNASISLAIRRSSGGKRTPPQIQRMASEDLIAVLFPDQIACAENLSGPREIPD
                     HPLVKQTLHDCLHDAMDIDGLRRLLVGLETGTIKVIAKDLIEPSPLAAEILNARPYAF
                     LDDAPLEERRTLAVSQRRWLDPESAADIGKLDPAAIAAVREQAWPEVANADELHDAMH
                     AMGVITEEEGASGGWARYLDELMDARRATRLHTASNISWVCAECLPLVQAVYPEARRV
                     PLVEPPAEKAEKIWQREEAITDLVRGRLQSVGPITARDMAATLGLTLSEINIALIELE
                     SEGFVLRGRFTEEASASEAVQQGDATLEWCERRLLARIHRYTIKTLRAEIEPVSGADF
                     MRFLLEWQGVTRQPKPEGVESLAAVVAQLEGYEIPAAAWEGDVLAARLNEYDPHWLDS
                     LCLSGRALWARLTPTKSVGAAPVRTTPIALVTRKNWSLWHSLAAAPQEEMQLTHPARA
                     LFDYFNTHGASFFDDLVSGTNLLRSQAETALGELVSAGLVNADSFSGLRALLIPSDKR
                     RQLVARRRRVALFGLEDAGRWSLIRKGATRNDAEALEQVAEILLRRYGVVFRKLLERE
                     ADWLPPWHALLRVFRRLEAQGKIRGGRFVAGMSGEQYALSDAVSSMRAIRRQETKGDL
                     VSLSAADPLNLTGIITPGARVPALANNRVLYRDGVPVAVHSGGQSEFIVPMEPEKEWE
                     ARQVLVRKRLQPGASRAARGDRPPH"
BASE COUNT         2554 a         4549 c         4570 g         2531 t
ORIGIN      
        1 aggctcgagc gcgcaggcgt atcttgactc tccggaattt tgaaggaacc gcatggacct
       61 gaaacttggc ggaaaactcg ccctcgtctc cggcagcacc gccggtatcg gttattccat
      121 tgccgaagcg ctggcggcgg agggcgcgcg cgtcatcgtc aacggccgtt cacaggctgg
      181 tgtggacgcc gcgctcgcgt ccttgcgcaa gacgactaac aaagatgcgc tcggcttcgc
      241 cggcgatctt tcggaagcgg cggtcgccga ggcccttgtc aaggcgcatc cgggcatcga
      301 gatcctggtc aacaatctcg gcattttcga gccgaaggcg ttcgaggaaa ttcccgacgc
      361 ggactggcgt cgcttcttcg atgtgaacgt gctatcgggc gcgcggctcg caaggctggt
      421 gctgcccgcc atgcgacgcg cgaactgggg gcgcatcatt ttcatctcca gcgaaagcgg
      481 cgtgcagatt ccggcagaaa tggttcacta cggcatgacc aaggccgcgc agatcgcagt
      541 cgctcgcggc attgccgaag ccgtcgcggg caccggcatc accgtgaaca gcgtgctgcc
      601 cggcccgacg aaatctcgcg gcgtcggcga tttcgtcgat gcgctcgcga aggaacaggg
      661 caagtccggc gcggaattcg agaaggaatt cttcgaacat gtgcgcccca cctcgttgct
      721 caagcgcttc agcacgccgc aggaagtggc gtcgctggtg gcgtacgtcg cgagcccgtt
      781 gtcttcggcc accacaggcg cagcgttgcg cgtcgacggt ggcacggtga aaagtgcgtt
      841 ctgaatgata gtgaagcgca gcaaggtcgt gaaaattccc ggcaatgaag aatggtcggg
      901 gtacaaggac gacctcgatg cacgtcacgc acacggcctc tggttcggaa agtcgctgga
      961 tgatatgcaa ccgctcttct ccggtggccg gtccatcgaa cgaggcagcg aactgttatt
     1021 catgcctcgt cgcgcatttc agttttacgt cttcgcgttc gcgcagtacg tgatgtcaga
     1081 ggcggccatc ggcgatccgg atggagcgag ctgcttcctc aattacctca cggcgcgcga
     1141 aaagcgcgat ccgggcagcg ttgccgcgat tttttcgcgt ctcgagcaga ccatcgactt
     1201 cgtagcggcg agccaggcgc gcttcgacgc cagccacgac atctatggtg acttcaccga
     1261 gaaagcggaa gatttcagga agctcatcgg cgcgactcac tcaacacacg accctgagga
     1321 tcaaatgctc gaccccacgg acgacgcctg atgaccactg aatacgaagc catgctcgag
     1381 gtcgcaatcg ccgaagcgaa gcgcggcctc gacgaaggcg gcatccccat cggcgccgcc
     1441 ctgtatcaca acgacggaag gttgttaggg tgcggtcaca accggcgcgt gcaggaaggc
     1501 gacccgtccg tgcacggcga aaccgacgcg tttcgcaagg ccggtcggca gcggcggtat
     1561 cgcgacacca tcatggtcac cacgcttgcg ccctgctggt attgcagcgg cctggtggtg
     1621 cagttccgga tcggcacggt ggtggtgggc gagtcgcgca cctttcaggg tggcatcgac
     1681 tggctgcgcg aacgcggcgt gaaggtggtc gacctgggct cgtacgagtg cgaaaagctg
     1741 ctcggcgact acatcgccgc caatcccgag gtatggaacg aagacatcgg cgaatgatcg
     1801 ccggcgttta cgccacgggc tccgcggccg gctcgatggt gtattcgagc gtcagttgac
     1861 agggcgtgcg gccatttttg tcacgaaccc gctgtccacc gcatttcgtg cttccgtggg
     1921 catgcggctc gcgtgaccgc gccagctcgg ccacggcgct ggtgaggtcg aactcaccgt
     1981 cgcaatcggc ataggggcag ggaaacacga aataggcctg tgacggcgga tgcatgacca
     2041 gcaggtgcgg cgccggggcg gaaatgtccg ggtccgattc gctgaagtcg aattccagct
     2101 tcaacgacgc gaactgcgga tacatcacgc gcatgacctg cgcggtggcc cgatcgcggc
     2161 gctgtcgctc gcgcagttga ttgcggctgt tggcgctggt gaatctcacg gcacagcctt
     2221 gggcgcagca ggcgtgatgt cggcagccgg cgccgcgaca tcgccaaccg ctccgggttc
     2281 gaccgagccg acctcgccag cctccggttc accggacacg cgcgccagtt tcttggccag
     2341 cttttcctgc tgccgggcct tgcgggcatt ttctttctgg cgctttgcct ggttgtagtt
     2401 agtcttagcc aaaggaacga tctccagccg ccgagagcag cgtcaattcg tgacaggcag
     2461 cggacgtgcc gggatttctc ccgggcacga agatgttgag acaggagtcg tctccataaa
     2521 gcatgcccgc ggcggtgcgc acgttggcga acggcccgag catcaccggg gcgtcgttcg
     2581 cacccatgga gaaccgcagc ttcacgggcc cttcgcagtc gagctcgcga tgcatgcggc
     2641 cgctcaatcg ccagcagccg tccacgcaac gcgctacgag atagttttcc ggaccccgca
     2701 acgttccatc cgcgtgaaaa cggaagaacg tggcaacggt gagcgtgggt gcgtcacctt
     2761 tgatggagcg gaagttcagt cgcagcatgt agcgtgggcg gtgcctcgtt ggctgccggt
     2821 cagcgccggt tgtagccgcc gccgccaccg ccgcgcggct tgtcctgcgc ttcgttcact
     2881 ttcagcgggc ggccgccgaa atcgcgacca ttcagcgact gcatggcctt ctgcgcgtcc
     2941 gcgttcgcca tttcgacgaa cccgaaacca cgcggctgac cggtatcgcg atcattgacc
     3001 agggcaacct tctcgacgtt accgtgctcg gcgaacaacg cccgcacaga gtcttcggtg
     3061 gcggagaagg gcagatttcc aacgtaaagt ttcatgcgat gagtcccaat gagcagtgga
     3121 ccgcagggat atcccgtcgg tcggttcgcg aatgctttga ggtgacagac tctggccggt
     3181 attgcgtctt gcgacgcggg tccagggacg gacaagacga aggatcacaa gccgggtctc
     3241 aaggtacccc aaatgcgcca atcgcacggg tctaaatggc gtttccatgt cgtttgcgcg
     3301 ccaaaattcg ccgccgaggc cctggacgag cgggttttca gtacccgcct ccggctgcgt
     3361 gctgcatcag cgtcgcgtga tcgcgacctc gaggtacgcg gcatccacga ccatggtgcc
     3421 gtcggtcgcg cgattgaatt tcgccaccag ctctaacagg tcggaacgca ggcttcgttt
     3481 ccggtccgcg tcgagcgacg cgaagatgcc gtggaccgga agataggcgg tgcgcagctt
     3541 gtccacccag tccatgggag tgcggctgcg gaatgcgacc cgcttgccgt ccacttcgat
     3601 atcgccgtag gcggcaaaca gatcctcgat gcgctcccgt gtgccccagt cgcagggcat
     3661 ggcggccggg cccggcggcg aatggcgaat cacggtcctg aacaactggc cgatgaagcc
     3721 gtccggtgtc cagttggtca gacccacctg cccgccgcgc cggcacaccc ggatcatctc
     3781 ggacgccgcg cgctcctggt cggccgcgaa catggcaccg aaacaggaca acacggcatc
     3841 gaagctctgg tcggcgaaag gcagcccgtc gacatctccg tcgacgatgc gcacgcccac
     3901 cgactcggct tcgagccgct gccggcctcg aggggccagg tccgtggcat ggtccgttgc
     3961 ggtcacctcg caccagcggc gtgcggcggc gagcgaggca gcgaaattcc ccgtggctac
     4021 gtcgagaacg cgccggtcct ggcataaatt cacggtttcg cagagttcct cgcttaccaa
     4081 ctgcagcgca ttgccggcga cggcataatc catgctcgcg tacggcaggt gccggcgtgc
     4141 gatgacggca cgatgatcga ttcgcggggc gtttctcgaa agaactgcgt tcatgacatc
     4201 cctcctcctt ccagattccg ctgtcaaagc tacggaacgc gcgtggtaac gagcgtggga
     4261 aagcccgtgg aatcgctcga aatccggctg ttgggcccgc tccagctggc tcgcggcggc
     4321 gaggttctga agctgccgtc gtcgcgcaaa gtgcgcgcgc tgttgggcta cctcgtgctg
     4381 gcgagccgcc cggtgacgcg ctcgcaggtc tgcgagctgc tgtgggacgt gcctaacgac
     4441 cctcgtggcg agttgcgctg gtgcctctcc aagatccgtg ggctgatcga cgagaagagt
     4501 cgaaagcgga tcatcgcgga cggcagcacc atccggctcg acctgtccga ctgcatcgtc
     4561 gacgcactgg aagtcacgcg tgcacccgaa cacggtatca agaagttagg cgtcgaacgc
     4621 cagcaggcgc tggtggcaca attcgccggc gaattgctcg aaggcctcga gatctcccgc
     4681 agccccatgt tcgatgcctg gatcacggcc gagcgccggc gctttcgcgg catccagacc
     4741 gtgctgctcg aaaacctcgc gcgtgcgttg ccgcacgagg ctgcggctcc ttacttggac
     4801 gagtggctca agctctcgcc tttcgaccga aatgcccacg aactgctgct cggcgcgctg
     4861 gcgcggcacg gacggattgc cgatggcgag gcgcacctcg catcggcctg caaactgttc
     4921 gaagccgatg ggctcgacag cgcgccgctg cacgagctct ggcgcaacac gcgcgagaaa
     4981 catgccgcgc caatgcagcc tgcacatgcg cagcccggcc ccgcacaact cgtcagcgcc
     5041 agcctcaccg agattcaccg attggcgccc gacgcttccg cggccacccg tcgcgcgtcc
     5101 atcgccgtca tgccgttcgt cgaccgctct tccgaagccg acaagcgcgg cggcgcggcc
     5161 gacgcactgg cctacgacgt catcacgcgg ctggcaaagc tccgcagcat gttcgtgatt
     5221 gcccagggca ccaccttcgc actgcgcgat cgcgccatcg ggcccgaaga agccgggcgc
     5281 atgctcaacg tggattacgt ggtcagcgga tctgtacggc gcaatggccg gaaattcacc
     5341 gtgagcgccg acctcattga aacgcgcacg gcgcgcatcg tctgggccga ggtgttcgac
     5401 gagaaggtgg acgacgccct cgacgtgctc gacgagatcg gcaaccggat cgtcgcgtcc
     5461 atcgcgcacg aaatcgaaat ggtggaacgc aatcgcgcgg tcctgaagcc gccgaattcg
     5521 ctcgacgcct gggaagcgca tcaccgcggc ctgtggcaca tgtaccgctt caacaaggcg
     5581 gacaacgaac gggcacggca gtttttttcg caggccgtgg agctcgatcc cacgttcgcg
     5641 cgcgcctatg ccgggctgtc gttcgcgcat ttccagaatg ctttcctcgg ctggaagaag
     5701 cctgcccccg aaatcgatcg cgccttcgag gccgcgggca agagcctgat ggtcgacgac
     5761 cgcgatccgg ccgcgcactg ggccatgggc cgcgcaatgt ggttgcgcgg cgggcatgac
     5821 caggcggtca ccgaactgga acgcgccgtg gacctctcgc cgaactacgc ctccgcgcac
     5881 tacacgctcg ctttcgtgca ctcgcagtcg ggggatccgc acgccgcgat ttcgttctcg
     5941 gatcattccc gccagctgtc gccgttcgac cctctgctgt tcgcgatgct cggctcgcgc
     6001 gccatcagcc tcgcgcggct ggggcaatac gaggaagcct ccgagtgggc catcaaggcg
     6061 gcggcgcgac ccaacgcgca ccaccacatc atggccatcg ccgcgttgtc gctcggcctc
     6121 gccgggcgca tcgaggaagc caacgagtac aaggatcgta tccgccagcg tgttcccgac
     6181 tatgcgatga cggacttcct ggcggcgttt ggctactcgc ccgaggcgga ggcgctgttc
     6241 aagcaggccg ggaggaaagt cgggattact tagcaaacgc cgccccggat acgggtcggc
     6301 gcccgccaga aatccgcaat tgacgatgct tcggttgctg tgatttattg gccgcgtcaa
     6361 gggggattca tgaaaacgat cggatgcttc atcgccatca cgttgttagg cctggcgcct
     6421 ggcgccttcg cccaggtcaa cattccgaat ccgtccgcgc cgggcaattc acccgatagc
     6481 gccgttcgcc tcgtcgccac cagcgagctc atggtagatc gctacatcag ccgctggatt
     6541 cggacccact acccgggctg gagcgccgat ccgcacgaat tcatggagat cggcttcgaa
     6601 cgctacgcgg tcgtgtacat cacctcgccg aacaacccgg gacgccggat ctacttccgg
     6661 gtcatgaaga accagaacga agacgaaggt tccggcatgc ctcctctctg acaaccttgc
     6721 tgcgttgcga ttgcgacgcg acgggtgcgc ggatttcgtc ggcgcaatct gatagctcgt
     6781 gtagtgcgca cggtacatac tccgcggtgc agtcgaaccg atgcggcccc ttcgaggtag
     6841 cgagcatgtc cactgacaac ccgtacgcgc agtatcacgt cgtaaagcag ggtgacacgc
     6901 tctcgaagat cgccgaggag tattacggcg acaagatgct ctatccgaag atctttgaag
     6961 ccaataagga cgtcctgcag gaccctaaca agataaaacc cggccaaaag ctgcggattc
     7021 cgtgatccac ccgttcgagg agaccccgat gcgttttgcg aagattttcg tggcgcttgc
     7081 cgccaccacc ctgatgattg ccggttgcgc cagccagaaa gaacccgccg aaaaagccat
     7141 ggcgcaggcc gagtcgtcag tcgccgaatt tcgcgcggat gccgaaaagt atgcggcgga
     7201 cgagctggcg aaggtcgatc aatccatcac caagctcaag aacgcgctcg ccaacaaaga
     7261 ctacaacgtg gtcgtgaagg cagttccgtc gctgaactcg acgattgcct cgctcaagca
     7321 gtcggtcgcc gccaagaaag ccgatgccga cgccatgatg gccgcggcga ccgcggagtg
     7381 gaccgaactc tctgcttcgg tgccgcagat ggtggaagcc attcagagcc gcgtcgatac
     7441 gctgggcaag tcgcgcaagc tgcccgccaa cgtcgacaag gcgacctggg aaacggtgaa
     7501 aaccgacttc gaaaccatga agaccgagtg gaccgacgcc accacgcagt tcgcctccgg
     7561 ccaggccgat gcggccgtgc gcaaggcccg cgaagtgaag gccaagggtg acgcaatcct
     7621 gggcaagctg gggatgactc cgaacgcctg atcgacgccg catcggtctc tcactcgaga
     7681 tcgatgcgtc cgacgatgac gcgcgtgaca cgcgccggca cggaaaactc gagcggcagg
     7741 aagtagtcag tccactcgcg cggcacgatg tagaacactc gcagcccggg cttctggaaa
     7801 tagctggcct tccaggtgtt caacatcgcc tccgcctcgt cggcaaaaag gccctgagcg
     7861 atcagggcct ttttcaatga gctgcgaagt tgcgctgcac cgtcggcggt gtagtcaccg
     7921 ccggaaaagc gtttgacctt cgccagcccc ttgcccgcgt tctccttctt cagcgttacg
     7981 gccggctgcg atcggaacgc gatgattccg tcggcgcgta catcggcgag ccagacgttg
     8041 ggaacgacgg ccgccgattc gagccaggcg agattggccg gcgtcgaaag ctccaccgaa
     8101 ccgcgcgtgg ttttcgtcgc cagcagcgca tcgagatgcg cgacgccgcg gtagaaaagg
     8161 tactgctcgc cttccccggc ttccgggctg aacacgctga ccgagttcac ttcgcgcggc
     8221 gcgagccaga tttcgcttcg ggtgagcggc gcgaccacgg tgtcgtgcag gcgcagccct
     8281 ttccagcgca gcccgccgac cacgtaattg ttgagcacat cgccgttcca ttcggtggtg
     8341 atgacgcctt cgtgcatctt gtccgcgatg cgctctcgat cgagcgccac cgacgcggcc
     8401 ccatccggat agaactcgtt gatcacgccg ccgcgaaacc gcaccgtgat gtcgataggc
     8461 gctgtgtacg ccttgttcgg cggcggatga aagtagatga ccggcgtttc caaccgcatg
     8521 gtgatatccg gccggcccgg aatgttgggc agcttgccga gaatgcgctc cggcttgtag
     8581 cgcgtggttt cgggttcgta gcgatgtacg aaatccggca atacatccgc tttgtcgatg
     8641 ctgttgagac cgcccgccgg agtgccgtcc gcggcgtgaa tggtggtgat ggtgccccat
     8701 tcgtggacga ccaggtccgc ggacagcgcc ggacgcagcg ccccggccag gattattccc
     8761 agcaacacca tccggaaatg gcgacccgat gcgttcatgg cggctccttg cggcattggc
     8821 cgcggaatag tgccgagcct gccatgtgac cggcgggcgc gcgcggagtt tcgcgccgca
     8881 gcgtcgcggc gcgaaactcc gcttacgccg gctacttttt cgctttcttc gcttcgagga
     8941 aagcggcgta cgccttcgac tccgctgcct gcgtggtgac cagcagctgg atctcatcac
     9001 ttacctgcgg gacgaacgcc ccgagcccga attccgagcg cttgagtgtg cccttcgcgt
     9061 cgaagccgat ggtcgccact tcggtacgcg cattcttgcc gaccttgagc aaggtcacgt
     9121 ccagcatcac cggccgggtg accttgcgaa tcgtgagatt gccggtgacc ttgaagcggt
     9181 tcgttcccat gcccttttcc acccgggtgc tgatgaaagt ggcggtcgga tacttcgcca
     9241 attcaaagaa gtcttcggat ttcaggtgtt catcgaggtc gggcacaccg gtgttcaacg
     9301 atgcgattgg aatggtcacc ttgatcgagg ccatggtcgg gtcgctctca tcgaaatcga
     9361 gagttcccgt gccctgcgcg aactgcgcgg cgggataaga gaagcccaga tgatcccagc
     9421 gcaacacgcc ctgggtgtag ttgggctcga gcgtgtacgt ggtagcgaat gcgggcaggc
     9481 caagaagcgc aagagtggtg gcgaacacga gtcggtacat ggaggtctcc ttcggttgga
     9541 tgaaaagccc gacgctgcgt ccaagccgct gaaaagtcct gaatccgcgc tgaaaatctc
     9601 cgattcgcgc tgaaggattc gggtcgccgt gttatatcca cgccactcat ggaccgtctc
     9661 accaccatcg cgcttcaagt cggggcctgg cgaatcgacc cgcaactcgg gcagatgtcg
     9721 cgaggagatc aaacggtgcg gctcgaagca cgcacgttgc gattgctgat gtacctcgcg
     9781 aaccgtgccg gcgagaccgt cggcatcgag gaattgctcg accaggtctg gtcgggtgtc
     9841 gtcgtgacgc aggactcggt gtaccaggcc gtcaccgcgc tgcgccgcct gcttggcgac
     9901 gatgcgaagc aaccgacata catcgtcacc gtccctcgcc tgggctaccg gctcatcgcg
     9961 ccggttcggg cggcggtgga agagcccgcg tcggccatac tgccgccctc gttgccgcca
    10021 gcgccggcga cgcgcgcgac tcaaatgcgg cgcagcgcgc ttttaggcgt gctcgtggcg
    10081 ctggcggggc tgggattcct tgcctggcgt aacagcatcg cgccggatgc gaaatccgtg
    10141 gccgtcatgc cattcctcga cctgaccagc caggcgatgg acgaagaata tttcgccgac
    10201 ggcctcaccg aagagctcat cgatcgcctg agccgcgagc ccggcttcaa ggtaccggcc
    10261 gcgagcgctt cgtacttcta caagaacaaa cagctgccgc cgtcacgcat cgcgcaggaa
    10321 ctgggcgttg tctatctact cgaaggaagc ctgcgcgaat ccggcgacac tctgcgcatc
    10381 acggcgcggc tcacgcgtgc ggcggacggc tacgtgatct ggacatcgag ctacgagcgg
    10441 tcgaagtcag accggctcgc catccaggac gagatcgcac tcaaggtcac cgctgccctg
    10501 cgcgactcgg tgcgttaatg aggagggcga tcgccgcgcg ccgcgcgcga ggcgcccggt
    10561 tgcaggcgtt tgcgcaccag cacctgtcgc gcctcccatt ctttctccgg ctccattggc
    10621 acgatgaact cggactgacc acccgagtgc acggcaaccg gtacaccgtc gcgatacagc
    10681 acgcgattgt tggcgagcgc cgggacgcgc gcgccgggcg tgatgatgcc ggtgaggttc
    10741 aacggatccg ctgcactgag tgacacgagg tcgcccttgg tctcctgccg ccggatcgca
    10801 cgcatggacg acacggcgtc ggacagcgcg tattgctcgc cactcatgcc ggcaacgaaa
    10861 cgcccaccac ggatctttcc ctgcgcttcc agccggcgga atacgcgcag cagcgcatgc
    10921 cacggcggca gccagtcggc ctcgcgttcg agcagcttgc ggaacaccac gccgtagcgg
    10981 cgcagcagga tctccgcgac ctgctctagc gcctcggcgt cattgcgcgt ggcgcccttg
    11041 cgaatgaggc tccagcgccc cgcgtcttcg agaccgaaca gcgcgacgcg gcggcggcgc
    11101 gccacgagct ggcgtctctt gtcgctggga atcagcagcg cgcgcagacc ggagaaactg
    11161 tccgcgttga ccaggcccgc cgacaccagt tcaccgagcg cggtctcggc ttgcgagcgg
    11221 agtagattgg tgccgctcac gaggtcgtcg aagaacgacg ccccgtgcgt gttgaagtag
    11281 tcgaacagcg cgcgcgcagg gtgagtcaac tgcatttctt cttgcggcgc cgcggccagc
    11341 gaatgccaca acgaccagtt cttgcgtgta accagcgcga tgggagtggt gcgcacaggc
    11401 gcggcgccca cggatttcgt gggtgtcagc cgcgcccaca gcgcgcgtcc tgataaacac
    11461 aggctgtcca gccagtgcgg gtcgtattca ttgaggcgcg cggccagcac gtcgccctcc
    11521 caggccgcgg ccgggatttc gtagccctcg agctgcgcga ccacggcggc caggctttcg
    11581 acaccctcgg gtttcggctg gcgcgtgaca ccctgccact cgagcaggaa gcgcatgaaa
    11641 tccgctcctg acacgggttc tatttcggcg cgcagcgtct tgatggtgta gcgatggatg
    11701 cgcgccagca gccgccgctc gcaccattcg agcgtggcgt cgccctgctg gacggcctcc
    11761 gatgcagagg cctcttcggt gaagcggcct cgcaagacga agccttcgct ttcgagttcg
    11821 atgagcgcga tgttgatttc agaaagagtg aggccgagag tggccgccat gtcgcgcgcg
    11881 gtaatgggcc ccacgctctg cagtcggccg cgcaccagat cggtgatcgc ttcctcgcgc
    11941 tgccagatct tctcggcctt ctcggcggga ggttcgacga gcggaacgcg ccgggcttcc
    12001 ggatacaccg cctggactag cggcagacat tccgcgcaga cccaagagat gttcgaggcg
    12061 gtatgcaggc gagtcgcgcg ccgcgcgtcc atgagctcgt cgaggtaccg ggcccagccc
    12121 ccactcgcgc cctcctcttc agtgatgacg cccatggcgt gcatggcatc gtgcagctcg
    12181 tcggcgttcg caacctccgg ccacgcctgt tcgcgaactg ccgcaatggc cgcgggatcg
    12241 agcttgccga tgtctgccgc gctttcgggg tcgagccagc ggcgttgcga gacggcgagc
    12301 gtgcgacgtt cctcgagagg cgcatcgtcg aggaaggcgt atggcctcgc gttgagaatt
    12361 tccgccgcga gcggcgacgg ctcgatgaga tccttcgcga tgaccttgat cgtgcccgtt
    12421 tcgaggccca ccaacagtct tcgcaggcca tcgatgtcca tggcgtcgtg caggcagtca
    12481 tgcagcgtct gcttcacgag cgggtgatcg ggaatctcgc gcggccccga caggttttcg
    12541 gcgcaggcaa tctggtcggg aaacagcacc gcgatcagat cctcggacgc catgcgctgg
    12601 atctgcggtg gggtgcgctt gccaccggag ctgcggcgga tggcgagcga gatgctcgcg
    12661 ttccagcgcc agcgcgcggt gaacatcggt gagtcgagca tcgcctggat cagcgtgtct
    12721 tcgacggtcc tgctgttgag gaagcgtgcc acgctcgcga gatcgaaact gtgggtctcg
    12781 cccagcgaca gcacgatggc gtcttcggtg gccgcggcct gcagctcgaa attgaaggtg
    12841 cggcagaatt tcttgcgcag cgacaaacca aaggcgcgat tgacgcgcgc cccgaacggc
    12901 gagtggatga tgaattgctg gccgccggac tcatcgaaga agcgctcgaa caccagcgtg
    12961 tcgcgcttgg gcagttcgcc gagcacggct ttcgcggcgg cgaggtattc caccatctgg
    13021 tgcgcggccg ggcgcggcag gtggtagcgc tcgatgacga tgtcgatcgc gcttgcgagg
    13081 gattccgccg tcacttccgg caacagtgtt tcgatgtcgg cacgcagtcg ggataccgat
    13141 tccgaaagct catcggtgcg cgcgggcgct tcgcccaacc agaacgggat gctgggcggc
    13201 tggcccttgg catcttccac gcgcacgcga cccgcttcga cgcgcaggat cttgtaagac
    13261 gtgttgccga gctggaaaac gtcacctgcg agactctcga tcgcaaagtc ttcgttgagg
    13321 gtgccgacga acacgcccgc gggctcgagg atgacctggt agtcggcatt gtcgggaatg
    13381 gcgccgccgc aggtgatggc ggtgagccgc gcgcctttgc gcgcgcgcag gcgctggttg
    13441 acggcgtcgc gatgtagata ggtgccgcgc cggccgcgct tggtgtcaat gccttcggcc
    13501 agcatgcgca ccacggcgtc gaagtccttg cgctccaggt tccggtacgg gtacgcgccg
    13561 cgcatgagcg cgaacaattc gtcctcgccg tattcgcgcg cggcgacttc ggcgacgatc
    13621 tgctgcgaga gcacgtcgag cgggtgttcg gggatgtgca gacgatcgag ctcggtgcgg
    13681 cggaccgaat cgagcaatgc cgtgcaatcg acgagttcgt cgcggctgct cgggaagatg
    13741 cggcccttgg gcacgccgcc cacggagtga ttggcgcggc cgacgcgctg caggaatacc
    13801 gagatgctgc gcggcgtgcc cagctggcag accagctcca cgtcgccgat gtcgatgccc
    13861 agttcgagcg acgccgtggc caccatgacc ttgagcttgc cggctttcag gcgctgttcg
    13921 gcgtcgaagc gggtttcctt ggacaggctg ccgtgatgcg ccgccacgtg ttcctcgccc
    13981 agccgctccg ccaggtggcg cgacacgcgc tccaccatgc ggcgcgtgtt cgcgaacacc
    14041 agcgtggtgt ggtgatcgct gatgagtgcg gccagctggt cgtacaccga ggcccagact
    14101 tctcccgaca tcaccgcctc gagcggcgcc cccgggatca cgatgccgat gtcgcgctcg
    14161 cggacgtgcc cgctgttgac aattgtgggg tcagatcgcg catc
//
LOCUS       DASQJS010000264         3781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1408019, whole genome shotgun sequence.
ACCESSION   DASQJS010000264 DASQJS010000000
VERSION     DASQJS010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408019"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..634
                     /locus_tag="VEW08_10675"
     CDS             167..634
                     /locus_tag="VEW08_10675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ41249.1"
                     /translation="MIADVAVRLATLSDAADIAAMSRDYIERGLPWTWTEERVAHSIR
                     DPETNVVVAGEHGAIIGFGIMFYASDDAHLLLFAVRRAHQRRGVGSAILRWLEDVARA
                     AGAKRIRVECTRENAAGRNFYCEHGYHELDIAKKYYRGLKDGVRLEKWLRENA"
     gene            complement(660..1544)
                     /locus_tag="VEW08_10680"
     CDS             complement(660..1544)
                     /locus_tag="VEW08_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ41250.1"
                     /translation="MSKRGWILFLSLGLLWGMPYLLIRIAVGEFDPLVVAGTRTLMGS
                     LLLLPIALHRKALAPAFRRWKWLLAFTLIEISVPWLLLGHAETRLNSSTAGLLIAVVP
                     LFAAIIVTKLGHESLEPRRMFGLGLGFAGVALLVGLDIHLDDLPAVAATIVTALCYAI
                     GPIIIDRKLNDVPAIGVITASLIVATIIYAPFAPFLWPETFSTPAVASVIGLGVLCTA
                     AAFIVFFELIAEVGPARATVITYVNPAVAILLGALVLKEPLTIGMAIGFPLVIVGSFL
                     GTMKKRSIPDETPAPATS"
     gene            1669..2439
                     /locus_tag="VEW08_10685"
     CDS             1669..2439
                     /locus_tag="VEW08_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41251.1"
                     /translation="MTFEYVSVEDAIQRRGLRMVVVGNVPSPWGEAAKGILHIKGIEW
                     AAVRLVYDSEPLKAWAGQRSGPVAIYNQDPPRSGWMQILMLAEELAPKPSLLPLQPTE
                     RGRVAALGEKFCGERGLGWMRRLQMAHAGLQKSGGFPEPIASYLGKKYGYDASVSETY
                     GARVRELLSEVGGVLRAQREAGKPYYLGDTLSAADVYSATFMALFKPLAEEYCRMEPA
                     VRAAFAWLDDATAAAIDPVLLEHRDMMYERHLELPLSL"
     gene            complement(2451..3368)
                     /gene="gcvA"
                     /locus_tag="VEW08_10690"
     CDS             complement(2451..3368)
                     /gene="gcvA"
                     /locus_tag="VEW08_10690"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HYJ41252.1"
                     /translation="MARRLPPLNALRAFEASARLGSFVAAASELRVSPAAVSQQVRRL
                     EDYLDTILFQRLARGLTLTDQGRDYLPELSAGFDLLGESTARVRAKRADGVLTLTTLA
                     AFANGWLLPRMHRFRERAPRIDVVLRTSRQVMDFRRDAIDLAIRFAPAPGRGLHGELL
                     SREELFPVASPQLFHGGCIPDTLAALAEYPLLHDTDAGIEQPWLGWRGWFERAGLSTA
                     TVGRGPQFSDSIVLIGAALAGLGIAIGRAPHVAPLLERGQLVRVTRETWTAPWSYYLT
                     APAAHFRRPLVRTFVDWALEEARNSSAPL"
     gene            3469..>3781
                     /locus_tag="VEW08_10695"
     CDS             3469..>3781
                     /locus_tag="VEW08_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HYJ41253.1"
                     /translation="MHLYDYLDSGNGYKVRLLLAQLGQKYDWTDVDIDKGQTRTPEFL
                     KRNPNGRIPTLELDDGTNLPESNAILWYLAEGSRFVPATQVGRAQVLQWMFFEQYSHE
                     PY"
BASE COUNT          758 a         1186 c         1252 g          585 t
ORIGIN      
        1 tcatggtgcc aatacgcgcc gtcggccacc gcatggatcg ggtcggcaag accgggttcg
       61 tgcggcagga aagctaggat cgcgataagg acgagttttc gattcatgcg ccaagtctag
      121 gaagcctgtg ttgcagactc atgccggcgc gtgtatctgt cggcacatga tcgccgacgt
      181 cgccgttcga ctcgccacgc tgtccgatgc cgcggacatc gccgccatgt cgcgcgacta
      241 catcgagagg ggtttgccgt ggacctggac ggaagagcgc gtggcgcatt ccatccgcga
      301 ccccgaaacc aacgtcgtgg tggccggcga gcacggcgcc atcatcggct tcggcatcat
      361 gttctacgcc agcgacgacg cgcatctgct gttgtttgcc gtgcggcgcg cacatcagcg
      421 acgtggcgtt ggcagcgcca tcctgcgctg gctcgaggat gtcgcacgtg ccgccggcgc
      481 aaagcgcatc cgcgtcgaat gcacgcggga aaatgcggcc ggccggaatt tctattgcga
      541 gcacggctat cacgagctcg acatcgcgaa gaagtattac cgggggctca aggacggcgt
      601 tcgcctggag aaatggttgc gcgaaaacgc ctgagcgcgc ggtgcggccg agacgggcct
      661 cagctggttg ctggcgccgg agtttcgtcc ggaatacttc gcttcttcat cgttcccagg
      721 aacgagccga caatgaccag cggaaacccg atcgccatgc cgatggtcag cggctccttc
      781 aacaccagcg cacctaacag gatggccacg gccggattca cgtacgtgat caccgtggcg
      841 cgcgcgggac ccacttcggc gatcagctcg aagaacacga tgaacgccgc ggccgtacac
      901 aacacgccaa gaccgatgac ggacgcgacg gcgggcgtgc tgaaagtttc aggccacagg
      961 aacggcgcga atggcgcgta gatgatcgtg gcgacgatga gcgaggcggt gatgacgccg
     1021 atggccggca catcgttgag tttgcggtcg atgatgatgg gaccgatggc gtagcagagt
     1081 gccgtgacaa tggtggccgc cacggccggt aggtcgtcca ggtgaatgtc gagaccgacg
     1141 agcaacgcga cacccgcaaa accgagtccc aggccgaaca ttcggcgcgg ttcgagactc
     1201 tcgtgcccga gcttcgtgac gatgatggcg gcgaaaagcg ggacaaccgc aatgagcaac
     1261 ccggccgtgg agctgttgag ccgcgtctcc gcgtggccga gcagcagcca gggcacgctg
     1321 atctcgatga gcgtgaaggc gagcagccac ttccagcggc ggaatgcggg tgccagcgcc
     1381 ttgcgatgca gcgcgatcgg caacagcagc aacgagccca tcagcgtgcg cgttccggcg
     1441 accacgaggg gatcgaactc gcccacggcg atgcgaatga gcaggtaagg catgccccag
     1501 agcaggccca gcgagagaaa caggatccat ccgcgttttg acatctccag attgtagaga
     1561 acgagggtgg cagaccgtta acaagcagag cttggtctcg aacaacgaaa actatttgac
     1621 tcggttcgcc acttgttgag cccgtcgcga tcgcagaagc tgcaggtcat gaccttcgaa
     1681 tatgtgagcg tcgaagacgc catccagcgc cgcgggcttc gcatggtagt cgtcggcaac
     1741 gtcccgagcc cctggggcga ggccgcaaag ggcatccttc acatcaaggg catcgagtgg
     1801 gctgccgtgc gcctggtgta cgacagcgaa cccttgaagg cgtgggcagg tcagcgcagt
     1861 ggtcccgtgg ccatctacaa tcaggacccg ccgcgctcgg gatggatgca gatcctcatg
     1921 ctcgccgaag agcttgcacc caagccctcg ctgctgcccc ttcaacccac ggaacgcggt
     1981 cgcgtagctg ccctcggcga aaagttctgc ggcgagcgcg gtctcggctg gatgcggcga
     2041 ctgcagatgg cccatgccgg actgcagaaa agcggcggat ttcccgaacc catcgcgtcc
     2101 tacctgggca agaaatacgg ctatgacgcc agcgtgtcgg aaacctacgg ggcccgcgtg
     2161 agagagctgc taagtgaagt cggcggtgta ctgcgcgcac agcgggaagc cggcaagccc
     2221 tactacctcg gcgacacgtt gagcgcagcg gatgtataca gcgcgacctt catggcgttg
     2281 ttcaagccgc tggcggagga atactgcagg atggagccgg ccgtccgcgc tgcattcgcc
     2341 tggctcgacg atgcgaccgc ggccgcaatc gatccggttc tgctcgagca tcgcgacatg
     2401 atgtacgagc gccacctcga gttgccgttg tcattgtagt cacggaccgc tcaaagcggc
     2461 gctgacgaat tgcgtgcctc ttccagcgcc caatcgacga acgtgcgcac tagcggccgg
     2521 cgaaagtgcg ccgccggcgc cgtgaggtag tagctccacg gagccgtcca ggtctcccgt
     2581 gtcacgcgca cgagctgccc gcgttccagc agaggcgcga catgcggtgc gcggccgatg
     2641 gcgatgccga gaccggccag cgcggcgccg atcaacacga tggaatcgct gaactgcggt
     2701 ccgcgaccga cagtggcggt agaaagcccg gcacgttcaa accagcctcg ccagccaagc
     2761 cagggctgct cgatgccggc gtcggtgtca tgcaacaacg gatattcggc cagcgcggca
     2821 agcgtgtcgg gaatgcagcc gccgtgaaag agctgcgggc tcgccacggg aaacaactct
     2881 tcgcggctga gcagctcgcc gtgcagcccg cggccgggcg cgggcgcgaa ccgaatggcg
     2941 aggtcgatgg catcacgccg gaagtccatg acctgccgcg acgttcgcag caccacgtcg
     3001 atgcgcggcg cccgctcacg aaaccggtgc atgcgcggca gcagccagcc gttggcgaag
     3061 gcggcgagcg tggtcagcgt gagcacgcca tcggcgcgct tggcgcgcac gcgcgcggtg
     3121 gactcgccca gcaggtcgaa gcccgcagac aattcaggta gatagtcgcg gccctggtcg
     3181 gtcagcgtga ggccgcgcgc gaggcgttgg aacagaatgg tgtcgaggta atcctcgagg
     3241 cgccgcacct gctggctcac ggccgcaggt gatacgcgca gctccgaggc cgcggcaaca
     3301 aagctgccga ggcgcgcaga agcctcgaag gcgcgcaacg cgttgagcgg gggaagtcgt
     3361 cttgccatgc cagagcaaga ataacttagc ctcagggaaa agcctcgtct ctcgcgccgc
     3421 cgccgcaatg gacggatact ttgttgccag acaaatacgg gatgcgccat gcacctctac
     3481 gactatctcg actcgggcaa cggctacaag gtgcgcctgc tgctggcgca gctcggccag
     3541 aaatacgact ggacggacgt ggacatcgac aagggccaga cccgcacgcc ggagttcctg
     3601 aagcgcaacc cgaacgggcg catccccacg ctcgaactcg acgacggcac gaacctgccc
     3661 gagtccaacg ccatcctctg gtacctcgcc gaaggctcac gattcgttcc ggccacgcag
     3721 gtcggccgcg cgcaggtgtt gcagtggatg ttcttcgagc agtacagcca cgagccctac
     3781 g
//
LOCUS       DASQJS010000265         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4236745, whole genome shotgun sequence.
ACCESSION   DASQJS010000265 DASQJS010000000
VERSION     DASQJS010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4236745"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="VEW08_10700"
     CDS             <1..1387
                     /locus_tag="VEW08_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HYJ41254.1"
                     /translation="NRAKILRIAFIYALAMLPVASHSALPPAVGETPMPTLAPMIKRV
                     SPAVVNIATRGTIRERSPRNPLLEDPLFRRFFDIPNMGPREREFQSAGSGVIFDAKNG
                     YIVTNAHVIDNATEITVTLQDGRDLTATVVGSDVPSDVAVLKVPSESLIQVALGDSTR
                     LEVGDFVVAIGNPFGLQHTVTSGIVSGLGRSGLNRDSYEDFIQTDASINPGNSGGALV
                     NLRGELVGINSAILSRSGGNIGIGFAIPVNMARSIMDQLLKYGSVKRGLLGVSIYSLT
                     PDVAKSLNIANTQGVLVSQVTEGSAADNAGIKPGDVITSINGQTIKSNGELRNAVGLS
                     RVGDKLDVALIRDRKPMHLTATITELPTPTASAARVNPAGASDSGVVHPGLAGATLAD
                     AAEGGVEIRAVEPRSAAAQMFRKGDLIEGANRQAIANLKELRAVARRGGALELKIRRG
                     NAMVLVPLRAP"
     gene            complement(1384..2115)
                     /locus_tag="VEW08_10705"
     CDS             complement(1384..2115)
                     /locus_tag="VEW08_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41255.1"
                     /translation="MRNLFRARPKSFLDAPRREWQFAVFAWMLRNCGGYPKFLDTTLV
                     LPIEEHFPDRDLTGHAAVAALFRRVRDHAGMADWPCTVEPEPADGRALPATSDRNPVI
                     TYKRGEFAAMSMAGAFAHELARMLVETFDEPPPGGAQLHEPAIDLAAVFMGFGVFMAN
                     STFEATRYDLNEGEFVHALALFCLLRKLEPESIARHLNPHLRKYLRLASRDLAQHEPD
                     FHKLRTVFAVVPFEVAERTLPTRVR"
     gene            complement(2198..>2674)
                     /locus_tag="VEW08_10710"
     CDS             complement(2198..>2674)
                     /locus_tag="VEW08_10710"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HYJ41256.1"
                     /translation="EADLAKYQKRRTRQAEGVGLVAKFRAELSKLYGMNLEPEVMRAR
                     KAQVFARLVSELHTLDERFGTESRLAAELEGKPNNARLASLATYYDCVPGFERLLAGE
                     QHDLPRFYAAARELAKLPREERRSRLCSGSSGSPALQSGAHVRLNEVQVLPAPTEG"
BASE COUNT          495 a          897 c          811 g          471 t
ORIGIN      
        1 caatcgagca aaaatccttc gcattgcgtt tatctacgct ctggcgatgc ttccggttgc
       61 aagccactcc gctttgcctc ctgccgtcgg cgaaaccccg atgccgacgc tcgcgcccat
      121 gatcaagcgc gtctcgccgg ccgtcgtgaa catcgccacg cgcggcacca ttcgcgagcg
      181 ctcgccgcgg aacccgctgc tcgaggaccc gctgttccgc cgtttcttcg acatccccaa
      241 catgggcccc cgtgaacgtg agttccagag cgcgggctcc ggtgtcatct ttgatgcaaa
      301 aaacggctac atagttacaa acgcgcatgt aattgataac gcgaccgaaa tcaccgtcac
      361 cctgcaggac ggccgcgatc tgaccgccac cgtcgtcggc agcgacgttc cctccgacgt
      421 cgccgtgctc aaagtgccgt cggaaagcct gatccaggtc gcgctcggcg actccacgcg
      481 gctggaggtc ggcgatttcg tggtcgccat cggcaacccc ttcggccttc agcacaccgt
      541 cacgtcaggc atcgtcagcg gactcgggcg ctccggcctc aatcgcgata gctacgagga
      601 tttcatccag accgacgcgt ccatcaatcc gggcaactcc ggcggcgcgc tggtaaatct
      661 gcgcggcgag ctcgtcggca tcaacagcgc catcctgtcg cgcagcggcg gcaacatcgg
      721 catcggcttc gccattccgg tcaacatggc gcgcagcatc atggaccagc tgttgaagta
      781 cggttccgtg aagcgtgggt tgctaggtgt gagcatctac tcgctgacgc ccgacgtggc
      841 caagtcgctc aacatcgcca acacgcaggg cgtgctggtg tcgcaggtca ccgaaggttc
      901 cgcggccgac aatgccggca tcaagcccgg cgacgtcatc accagcatca acggccagac
      961 gatcaagtcc aacggcgagc tgcgcaacgc cgttggcctc tcgcgcgtcg gcgacaagct
     1021 ggacgtggcg ctgatccgcg atcgcaagcc catgcacctg acggccacca tcaccgaact
     1081 gccgactccg accgccagcg ctgcgcgagt caatcctgcc ggggcatccg attccggtgt
     1141 ggtgcatccc gggctcgcag gcgccacgct cgcggatgcc gccgagggcg gcgtcgagat
     1201 tcgcgccgtc gagccgcgca gcgccgccgc acagatgttt cgcaagggtg atctgatcga
     1261 aggcgccaac cgccaggcca tcgccaacct gaaagagttg cgcgctgtcg ccagacgtgg
     1321 cggcgcgctg gaactcaaga ttcgccgcgg caacgcgatg gtgctcgtgc ccctcagggc
     1381 tccttaacgc accctggtgg gcagcgtgcg ctccgcgact tcgaagggca ccaccgcgaa
     1441 caccgtacgc aacttgtgga agtccggctc gtgttgcgcg agatcgcgcg acgccaggcg
     1501 caggtatttg cgcaggtgag ggttgagatg ccgggcaatc gactccggct cgagttttct
     1561 caacaggcag aaaagtgcga gcgcgtgcac gaactcgcct tcattgagat cgtagcgcgt
     1621 ggcttcgaac gtcgagttgg ccatgaacac gccaaagccc atgaacaccg cggcgaggtc
     1681 gatcgccggt tcgtgcagct gcgcaccgcc cggtggcggc tcgtcgaaag tttccaccag
     1741 catgcgcgcc agttcgtgcg caaacgcccc ggccatcgac atggccgcga actcgccgcg
     1801 cttgtacgtg atgacgggat tccggtccga ggtggccggc agcgcacgcc catccgcggg
     1861 ttcgggttcc accgtgcacg gccagtcggc catacccgcg tgatcacgca cacggcgaaa
     1921 cagcgcggcg acggcagcgt gcccggtcag atcgcgatcg ggaaagtgtt cctcgatcgg
     1981 caacaccagc gtggtatcga gaaatttcgg ataaccaccg caattgcgca gcatccaggc
     2041 aaacacggcg aactgccatt cgcggcgcgg cgcatcgaga aacgatttgg gtctcgccct
     2101 gaaaaggttg cgcatgtatt gcggccgatg atgacgggcg caatacacgc ctgcacgcga
     2161 atttgtcgac ctgacataag gggaaaaaat ccgcgtctca gccctcggtt ggggccggca
     2221 acacttgtac ttcgttgagt cttacgtgtg cgcccgattg cagcgctgga gatccggaag
     2281 agccgctgca caaacggctt cgccgttctt cacgcggtag tttggccagc tcgcgcgccg
     2341 ccgcataaaa gcgcggcaga tcgtgttgtt ctcccgccag cagtcgttcg aaccctggca
     2401 cgcagtcgta gtaagtggcg agcgacgcca ggcgcgcatt gttgggtttg ccttcgagtt
     2461 ccgccgccag gcgtgactcc gtgccgaacc tctcgtcgag tgtgtgcaac tcggacacca
     2521 gccgcgcaaa aacctgcgcc ttgcgcgcac gcatgacttc cggttctagg ttcatgccgt
     2581 acaacttgct gagctcggcg cgaaacttcg ccaccagccc gacgccctcc gcctggcgcg
     2641 ttcgccgctt ctgatacttg gcgaggtcgg cttc
//
LOCUS       DASQJS010000266         5440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3304172, whole genome shotgun sequence.
ACCESSION   DASQJS010000266 DASQJS010000000
VERSION     DASQJS010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3304172"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VEW08_10715"
     CDS             complement(<1..818)
                     /locus_tag="VEW08_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003849026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HYJ41257.1"
                     /translation="MRMDKLTSRFQAALSDAQSLAVGRDQQFMEPAHVLGALLDQQDG
                     STRPLLVKAGANVARLRKDLDAAIDKLPTVQGTPGDVHVSQDLNRLLNVTDKLAQQRK
                     DDYISSELFVLASFEDRNTLAKLLKDAGASKAAIEKAIEEVRGGEAVNDPQAEEKRGA
                     LEKYTIDLTARASSGKLDPVIGRDDEIRRTVQVLARRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGKRILALDMGSLIAGAKFRGEFEERLKGVLNDLSKQEGQV"
     gene            complement(878..1690)
                     /locus_tag="VEW08_10720"
     CDS             complement(878..1690)
                     /locus_tag="VEW08_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HYJ41258.1"
                     /translation="MTNLLWIILATTVSGVLSVLAAGIFLALPARAREGALPHLVSFA
                     TGALLGAALLGLIPHAVEGAGMEKVHEVGIALIAGIALFFVLEKFLLWRHCHDDHCVE
                     HPVGDAHGHDHAHQHEQARKKASGALVLVGDALHNVLDGVLIAAAFLTDVHLGIVTAL
                     AIMAHEIPQEVGNFAVLLNSGISRRRALVLNLLTSLTAVIGGIVGFFALKQALVALPF
                     ALAVAAASLLYVAVADLIPGLHRRVDPRSSVTQVVLIGLGIALIAFAERNAH"
     gene            complement(1715..2446)
                     /gene="pgeF"
                     /locus_tag="VEW08_10725"
     CDS             complement(1715..2446)
                     /gene="pgeF"
                     /locus_tag="VEW08_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HYJ41259.1"
                     /translation="MSLLEFDWRLPAGVHAAFTTRLGGVSSAPWDSFNVATHVGDVAK
                     DVAANRARLGTLLALPSDPAWLSQVHGTVVADLDAYPQGVITADAAVARTDGRVCVVM
                     VADCLPVLFASRDGRRIAAAHAGWRGLAAGVIEQTVAAMGVRGDVLTAWLGPAISKEH
                     FEVGDDVRAAFVDADSGAADAFVRNANGRWQADLVGLARRRLAALDITDVSGGQWCTF
                     ADRERFYSHRRDGKGGRMAALIWRN"
     gene            complement(2443..3411)
                     /gene="rluD"
                     /locus_tag="VEW08_10730"
     CDS             complement(2443..3411)
                     /gene="rluD"
                     /locus_tag="VEW08_10730"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HYJ41260.1"
                     /translation="MPSKFQTHRFDLPPDFAGKRLDQALAQLLPQYSRTRIQRWIEEG
                     AVRINGLGVRARDLVTGGESATVEARLRPESGVAAEKLPLDIVHQDAQVLVINKPPGM
                     VVHPGAGNREHTLQNALLAHDAKLKRVPRAGLVHRIDKDTSGLLVVARTLEAHTALVA
                     ELGAHEIEREYLALCTGAMTGGGTVDEPIGRHRTQRTKMTVRSDGRKAVTHFRIERRF
                     RAHTLARVHLETGRTHQIRVHMAHVGYPIVGDPAYGGRRKLPAGASPELVNALQTFPR
                     QALHAARLTLTHPKSGKRVSFDAPLPEDLAGLLDALARDLAEPRRK"
     gene            3523..4323
                     /locus_tag="VEW08_10735"
     CDS             3523..4323
                     /locus_tag="VEW08_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HYJ41261.1"
                     /translation="MRTAGSLALLIFALGLSLTGCKSRDGKDAPMTDPAVVYERAHKA
                     LNQSDYPTAIRIYEALMARYPFAAETRQSRLDVIYAYYRAGESESAIDAADTFISENP
                     THPRVDYAWYLKGLTDFERLPNFLERWFRVDLDKRPPSQARRSFLSFSTVVEQFPNSE
                     YAHDARRRMVYLRNRLADYEIAVARYYVGRGAYVAAAQRAKVAIEEFDGAPAVRQALE
                     IMVYCYDQMDLKELAAQTRAMYRNNYEGEVGENKGPAKKKWYKLWLAS"
     gene            4470..4799
                     /locus_tag="VEW08_10740"
     CDS             4470..4799
                     /locus_tag="VEW08_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41262.1"
                     /translation="MLSWFNATEFQRFGEELAALFMEDKPADGEGNRKHRRMLKVKGK
                     SKGRDPVQDTLRKLVIRISAFEAKKGANFYKKAKFANAFKWKLKEAGYDAGFADELAK
                     ELLIHFR"
     gene            4889..>5440
                     /locus_tag="VEW08_10745"
     CDS             4889..>5440
                     /locus_tag="VEW08_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ41263.1"
                     /translation="MAETPPDSQALISAGNAAEDQGHPADALKLYDQAIAIAPEFARA
                     HLNRGNALMALQDFDAAITAFETAFRHDPALAGAQYNLGNAFARQRRYEEACSAYRRA
                     LSLKPGFVDADVALGSALDELGKMEDAVLHYRRALQAQPGLAPVYGNLGAVLIKQRQF
                     AEAEACFHRALELDPKLTHLYYRL"
BASE COUNT          982 a         1776 c         1724 g          958 t
ORIGIN      
        1 acctggcctt cctgcttgga cagatcgttg agcacgccct tgaggcgttc ttcgaactcg
       61 ccgcggaact tcgcgccggc gatcagcgaa cccatgtcga gcgccaggat gcgcttgccc
      121 ttgagccctt ccggcacttc gccgttgatg attctttggg cgagtccttc cactattgcc
      181 gtttttccca cgcccggttc gccgatgagc accgggttgt tcttcgtgcg gcgcgccagc
      241 acctgcaccg tgcggcggat ttcgtcgtcg cggccgatga ccggatcgag cttgccgctg
      301 gaagcgcgcg ccgtgaggtc gattgtgtat ttctcgagcg cgccgcgttt ctcttcggcc
      361 tgcggatcgt tcacggcttc accgcctcgt acttcttcga tggctttctc gatggcggcc
      421 ttcgaggcgc cggcatcctt gagtagtttg gccagcgtgt tccggtcttc gaaggacgcg
      481 agcacgaaca actcactcga gatgtagtcg tccttgcgct gctgggcgag cttgtcggtg
      541 acgttgagca ggcgattgag gtcctgcgat acgtgcacgt cgccgggcgt gccctgcacg
      601 gtaggcagtt tgtcgatggc cgcgtcgagg tccttgcgca gcctggccac attggcgccg
      661 gccttgacca gcagcgggcg cgtgctgccg tcctgctggt ccagcagcgc acccagcacg
      721 tgggcgggct ccatgaactg ttgatcgcgc ccgacggcca gggactgggc gtcggaaagg
      781 gctgcctgga aacggctggt gagtttgtcc attcgcatag gcggccaaaa tggggcccgg
      841 aagcgaacct ttcaataggg ccgagccctt atgcgggtca gtgggcgttc cgttctgcga
      901 atgcgatcag cgcgatgccg aggcctatca gcaccacctg ggtcacgctc gagcgggggt
      961 ccacgcgccg gtgcaggccc gggatcaggt ccgccaccgc cacatataag agacttgccg
     1021 ccgccaccgc caacgcgaac ggcagggcca ccagcgcctg tttcagcgcg aaaaatccaa
     1081 cgatgccgcc gatgaccgcg gtcaggctcg tcaacaggtt caggaccagc gcgcgccgcc
     1141 gtgagatacc cgaattcagc agcaccgcga agttgccaac ctcctggggg atttcgtgcg
     1201 ccatgatggc cagtgccgtg acgatgccca ggtgcacatc ggtcaggaag gccgccgcga
     1261 tcagcacgcc atccagcacg ttgtgcaagg catcacccac cagaaccaaa gcgccactcg
     1321 ccttcttgcg agcctgctcg tgctgatggg cgtggtcatg cccatgggcg tcaccgacgg
     1381 gatgctcgac gcagtgatcg tcgtggcagt gccgccagag caggaacttc tcgagcacga
     1441 agaacagcgc gatgccggcg atcagcgcaa tgcccacctc gtgcaccttt tccatgcccg
     1501 cgccctcgac ggcgtgcgga atcagcccca ggagcgccgc gcctaacaag gcgccggtgg
     1561 cgaaactgac caggtgcggc agagcacctt cacgtgcgcg cgcgggcagg gccaggaaaa
     1621 tgccggccgc gagcacggac agcacacccg aaaccgtggt ggcgaggatg atccagagaa
     1681 ggttggtcaa agcgcgagga tcctagcacg caagtcagtt gcgccagatg agcgcggcca
     1741 tgcgcccgcc tttcccatcg cggcgatggg aatagaaccg ctcgcgatcc gcgaacgtgc
     1801 accactgccc gccgctgaca tcggtgatgt ccagtgccgc cagccggcgg cgcgccaggc
     1861 ccacgagatc cgcctgccag cgcccgtttg cattgcgcac gaaagcatcc gccgccccgg
     1921 aatccgcgtc gacaaatgcg gcccgcacgt catcgcccac ttcgaagtgt tccttggaga
     1981 tcgcaggccc aagccatgca gtgagtacgt cgccgcgtac ccccatcgcc gccacggtct
     2041 gctcgatcac tccggcggcg agcccgcgcc agcccgcatg cgcggcggcg attcgccgcc
     2101 catcgcggct ggcgaaaagc acgggcaggc aatccgccac catcaccaca cagacgcgac
     2161 cgtcagtgcg cgccactgcg gcatccgcgg tgatcactcc ttgcgggtat gcatcgagat
     2221 ccgcaacaac cgtgccgtga acctggctga gccaggcggg gtccgagggg agcgcgagca
     2281 gcgtcccgag tcgcgcgcga ttggcggcga cgtctttcgc cacgtcaccc acatgcgtcg
     2341 ccacattgaa tgaatcccac ggcgccgagc tcacgccgcc caagcgcgtg gtgaacgccg
     2401 cgtgcacacc cgcgggcagg cgccagtcaa attccagcag actcatttcc gccttggctc
     2461 cgcgagatct ctcgccagcg catctaacaa cccggcgagg tcctcgggca gcggcgcatc
     2521 gaaggacacg cgcttgccgg acttgggatg tgtcagcgtg agccgtgccg cgtgcagcgc
     2581 ctggcgcggg aatgtctgca gcgcgttcac cagctcgggt gatgcgccgg ccggcaactt
     2641 tcggcggccg ccgtacgccg gatcgccgac gatgggataa ccgacgtgtg ccatatgcac
     2701 tcggatctgg tgcgtgcgtc cggtttcgag atgcacgcgc gccagcgtgt gggcgcgaaa
     2761 gcgccgctcg atgcggaagt gcgtcacggc tttacgcccg tcgctgcgca ccgtcatctt
     2821 cgtgcgttgc gtgcggtggc ggccgatggg ttcgtccacg gtgccgccgc ccgtcatggc
     2881 gccggtgcac aacgcgagat actcgcgctc gatctcgtgc gcgccgagct ccgccacgag
     2941 cgccgtgtgc gcctcgagcg tgcgcgcgac gactaacaac ccgctggtgt ccttgtcgat
     3001 gcgatgcacg agtcccgcac gcggcacacg cttgagcttc gcgtcatgcg ccagcagtgc
     3061 gttctgaagc gtgtgctcgc gattgcccgc gcctggatgc accaccatgc ccggtggctt
     3121 gttgatgacc agcacctgtg cgtcctggtg aactatgtcg agtggcagtt tttcggccgc
     3181 gacgccgctc tccggccgga gccgggcttc gaccgttgcg gattcgccac ccgtgaccag
     3241 gtcgcgcgcg cggacaccaa gcccgttgat ccgcaccgcc ccctcctcga tccagcgctg
     3301 gatgcgcgtg cgcgagtact gcggcaggag ctgggcaagt gcctgatcca gccgctttcc
     3361 ggcgaaatcc gggggcaggt cgaagcgatg agtctggaac ttggagggca accttttggt
     3421 ccgatggctg ggcggggccg ctatacttca cgcgtccggc gaccatttca aatcaaagac
     3481 accggcaaac ccgagaaacg gcaaaaacct tccatgcgag caatgcgtac cgccggtagc
     3541 ctggcacttc tgattttcgc tcttggtttg agcctcacgg gctgcaagtc ccgcgatggc
     3601 aaagatgccc cgatgacgga cccggcagtg gtctatgagc gcgcccacaa agccctcaac
     3661 cagtcggact acccgacggc catccggatt tacgaagcac tcatggcgcg ttacccattc
     3721 gcggcggaga cgcgccagtc acggctggac gtcatctatg cctattaccg ggcgggcgag
     3781 tcggagtcgg ccatcgacgc cgcagatacc ttcatctccg agaatcccac ccaccctcgc
     3841 gtcgactacg cctggtatct gaaggggctc accgatttcg aacggttgcc caatttcctg
     3901 gagcgctggt tccgcgtcga tctggacaag cgtccacctt cgcaggcgcg caggtcgttc
     3961 ctgtcgttct ccacggtggt cgagcaattc cccaacagcg agtatgcgca cgacgcgcgc
     4021 cgccgcatgg tgtacctgcg caatcgcctc gccgactatg agattgccgt agcgcgctat
     4081 tacgtgggac gcggcgcgta tgtcgccgcc gcgcagcgcg ccaaggtggc catcgaggaa
     4141 ttcgacggcg cgcccgcggt gcgccaggcg ctcgagatca tggtgtactg ctacgaccag
     4201 atggatctca aggagctcgc cgcgcagacg cgcgccatgt atcgcaacaa ctacgaaggc
     4261 gaagtgggcg agaacaaggg gccggccaag aagaagtggt acaagctctg gcttgctagc
     4321 taatggggtc aggccccatt ccctagctaa agcaagacga tgtcggcaga ggccggttga
     4381 ttgaaggtac gtgccacttc tcttaggatc acgccaccat ccagtaggca gacttgggtc
     4441 tgtcttcgca ttcttccgcg gagcgacaca tgttgtcttg gtttaacgca acggaattcc
     4501 agcgctttgg tgaggaactg gcggcactgt tcatggaaga caagccggcc gatggtgaag
     4561 gcaatcgcaa gcaccggcgt atgctcaagg taaagggcaa gagcaagggt cgtgacccag
     4621 tgcaagacac gctgcgcaaa ctggtgatcc ggatctcggc attcgaagca aagaagggcg
     4681 ctaactttta caagaaggcg aagttcgcca atgcattcaa gtggaagctc aaggaagccg
     4741 gctacgacgc ggggtttgcg gatgaactcg ccaaggaact gctgatccac ttccgttgag
     4801 cttgcgggcc ggaaggcagg aggatccagc attgggtttg ttcgatcgat tcaccaggcc
     4861 cgttggtcgc gcgcccgtgg agacgccgat ggcggaaacg ccaccggatt ctcaggcgct
     4921 catttccgca ggcaacgcag ccgaagatca gggccacccc gcggacgcgc tgaagctcta
     4981 cgaccaggcc attgccatcg caccggaatt cgcccgtgca cacctgaacc gcggcaatgc
     5041 gttgatggca ctgcaggact tcgatgctgc catcacggcc ttcgagacgg catttcgcca
     5101 tgacccggcg ctggccggcg cgcaatacaa cttgggcaat gcatttgcgc gccagcggcg
     5161 ctacgaagag gcttgtagcg cttaccgccg ggcgctgtcg ttaaagcccg ggttcgtgga
     5221 cgcggatgtg gccctgggtt cggcgctcga tgaactcggg aagatggaag acgccgtgct
     5281 ccactaccgg cgtgcgctgc aggcgcagcc tggacttgcc ccggtttacg gcaatctcgg
     5341 cgccgtgctc atcaagcagc ggcagtttgc cgaggccgaa gcgtgcttcc accgcgcgct
     5401 ggaactcgac cccaagctga cgcacctgta ctaccggctc
//
LOCUS       DASQJS010000267         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3306321, whole genome shotgun sequence.
ACCESSION   DASQJS010000267 DASQJS010000000
VERSION     DASQJS010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3306321"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VEW08_10750"
     CDS             complement(<1..302)
                     /locus_tag="VEW08_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYJ41264.1"
                     /translation="MQERYEAAAVERAAQDFWDNERVFEAREEVEKDTGDHTGKFYCL
                     SMFPYPSGRLHMGHVRNYTIGDVLTRFAKMQGKSVLHPMGWDAFGLPAENAAIKNGV"
     gene            456..887
                     /locus_tag="VEW08_10755"
     CDS             456..887
                     /locus_tag="VEW08_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41265.1"
                     /translation="MPYLLQAAFWVGVIGALAGTPLLFVLRFQNSHMTTGSFMWSSPA
                     VLFLLASITFVATYLLLPPNRWRTRVAGLSAGVLTFFGFAIWMSIMVNVRTSGYQTPW
                     PTVIYPFVIAFVIVGWIPVCGGWFAGWLVSRLPDAPAEEAA"
     gene            884..1174
                     /locus_tag="VEW08_10760"
     CDS             884..1174
                     /locus_tag="VEW08_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41266.1"
                     /translation="MNRSLFNNPSAAIPLGMSAGAFIMVLIHVISIGANPAADEGTAA
                     KLFQLLLAGQAPFVAFFALRWLPRMPSEAMYVIATQAAAALVALAPVVLFDL"
     gene            1237..2136
                     /locus_tag="VEW08_10765"
     CDS             1237..2136
                     /locus_tag="VEW08_10765"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYJ41267.1"
                     /translation="MGECEKLERTLERYQERFARVLAHIEDHSESALSTDDLSGIAAF
                     SRHHFHRQFSGFLGMSAYRYLQFMRMRRASWRLAFRPELSVNEIALSTGYEGPEAFAR
                     AFRQYAGRSPSEFRDRPKWPEWHSRFRPFMHLRQITMKPITAPGPVRVATFPATRVGK
                     LEHRGSAMHLGDSIRRFIDWRRANKLPPHLSATFNIVHNDPDETPSEDFRFDLCAATD
                     LAIEPNEAGVVEFVIPAGRCAVLRHRGSDDQLGRSIMHLYASWLPTSGEELRDFPLFM
                     QRLKFFPDVPDGEAETDIFLPLK"
     gene            complement(2198..>2875)
                     /locus_tag="VEW08_10770"
     CDS             complement(2198..>2875)
                     /locus_tag="VEW08_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HYJ41268.1"
                     /translation="LSFAIPIDVANNVRQQLVSTGKVTRGRIGVQIQDVNASFADSFG
                     LDRPRGALVTQVIDGGPSEKAGVKAGDIILSVNGKGVERSTALPGVISAIKPGDTAKI
                     EVWRERDSKILSVKVEEFQEETQKVANRDIEEPAKADKLGLSVRPLGADERKSAETEG
                     YLLVEDVTGPAAQAGVRPGDVILGVNGKAVKSIAELKNATANGSKTVAILLEREGAQL
                     FLPIRIS"
BASE COUNT          484 a          906 c          896 g          589 t
ORIGIN      
        1 acgccattct tgatggccgc gttctcggcg ggcagtccga acgcgtccca gcccatggga
       61 tgcaacacac tcttgccctg catcttcgcg aagcgcgtga gcacatcgcc gatggtgtag
      121 ttgcgcacgt ggcccatgtg cagacggccc gacggatacg ggaacatgga caggcagtag
      181 aacttcccgg tgtggtcgcc ggtgtccttt tctacttcct cacgcgcttc gaagacgcgc
      241 tcgttgtccc aaaagtcttg tgcagcacgc tcgaccgcgg ccgcttcgta tcgttcttgc
      301 atcgggaatg tctggttttt caggggcttg agaaggccgg gaagaataca cgacgcgggc
      361 tgcacaagcg aaaaagccgt tgaaactgtt gcgcaccatc ggggaacgtg gggcgcgtcg
      421 tcagcgagta ctcgtccaca ccatctgagg gaacaatgcc ttatctcctg caagccgcgt
      481 tctgggtcgg cgtcattggc gccctggccg gcacgcccct gttgtttgta ttgcgcttcc
      541 agaactccca catgaccacc ggttcgttca tgtggagttc gccggccgtg ttgttcttgc
      601 tggcgtcgat cacctttgtg gcgacctacc tccttctgcc gcccaatcgc tggcgcacgc
      661 gagtcgcggg tttgagcgcc ggagtgctca cgttcttcgg gttcgccatc tggatgtcca
      721 tcatggtcaa cgtgcgaacc agcgggtacc agactccctg gccgaccgtg atttacccgt
      781 ttgtaatcgc gttcgtgatc gtgggctgga tcccggtctg tggcggctgg tttgcgggct
      841 ggctggtgtc gaggctgccg gacgcaccgg cggaggaagc cgcgtgaacc ggtcgctgtt
      901 caacaaccca agcgccgcga ttccgctcgg catgtcggct ggcgcattca tcatggtgct
      961 cattcacgtc atcagcatcg gcgccaaccc ggcggccgat gaaggcacgg cggcgaagct
     1021 gttccagttg ctgctcgccg ggcaggcgcc atttgtcgcg ttcttcgcgt tgagatggct
     1081 gccgcgcatg ccgtcggagg ccatgtacgt gatagcgacg caggcggcgg cggcgctcgt
     1141 ggcgctggcg ccggtcgtgc tcttcgacct ctgagagcat acgaacagca acaagcgtca
     1201 ggcgcacgcg gctgccgcgc agctaaagtt cagcccatgg gcgaatgcga aaaactcgag
     1261 cggaccctgg aacgctacca ggagcgcttt gcgcgcgtgc tcgctcacat cgaagaccat
     1321 tcggaaagcg cgttgtcgac cgacgacttg agcggcatcg ccgcgttctc gcgccatcac
     1381 tttcaccgcc agttctcagg ctttctcggg atgagcgcgt atcgctactt gcagttcatg
     1441 cgcatgcgac gtgcgtcgtg gcggctggcg ttccggcccg agctgtccgt gaatgagatc
     1501 gcactctcaa ccggttacga aggtcccgaa gcctttgcgc gcgcattccg ccagtacgcc
     1561 ggccggtcgc ccagcgagtt ccgcgatcgg ccgaaatggc cggaatggca ttcgcgtttc
     1621 cggccgttca tgcatttgag gcaaatcacg atgaaaccca ttaccgcgcc aggacccgtg
     1681 cgagtggcca catttcccgc gacgcgcgtc gggaagctcg aacatcgcgg cagcgcgatg
     1741 cacctcggcg acagcatccg cagattcatc gactggcgca gagccaacaa gttgccgccg
     1801 cacctgagcg cgaccttcaa catcgttcac aacgatcccg atgaaacgcc gtcggaagat
     1861 ttccgatttg acctgtgcgc ggccacggat ctcgcgattg agccgaacga agcgggagta
     1921 gtcgagttcg tcattcccgc tgggcgctgc gcggtgttgc ggcatcgcgg ctcggacgac
     1981 cagctcggcc gctcgatcat gcatctgtac gccagttggt taccgacgag cggtgaagag
     2041 ctgcgcgact tcccgttgtt catgcaacgg ttgaagtttt tccctgatgt gcccgatggc
     2101 gaggcggaga cggacatctt tctgccgctg aaatgaaaaa gggccaggcc cgtgaggcct
     2161 gacccctttt gctgggaaaa gggagctgcc ccctttgtca gctgatgcgg atgggcagga
     2221 acagctgcgc gccctcgcgc tcgagcagga tggcgacggt cttggaaccg ttggccgtcg
     2281 cattcttgag ctcggcgatg gacttcaccg ccttgccatt gacgccgagg atgacgtcgc
     2341 cgggacgcac gccagcctgc gcggccggac ctgtgacgtc ttcgaccagt agatagccct
     2401 cggtttcggc gctcttgcgc tcatcggcgc cgagcggacg caccgacagg cccagcttgt
     2461 cagccttggc cggttcttcg atgtcgcggt tggcaacctt ctgcgtttct tcctggaact
     2521 cttcgacctt gacgctgagg atcttgctgt cacgctcgcg ccacacttcg attttcgcgg
     2581 tgtcgcccgg cttgatggcg gaaatgactc cgggcagtgc cgtggaacgc tccacaccct
     2641 tgccgttgac gctcaggatg atgtcaccgg ctttcacacc ggccttctcc gacgggccgc
     2701 cgtcgatgac ctgggtcacc agcgcgccgc ggggacggtc gagaccgaac gaatcggcga
     2761 aactcgcatt cacatcctgg atctgcacgc cgatgcggcc gcgcgtcacc ttgccggtgc
     2821 tcaccagctg ctggcgcacg ttgttggcca cgtcgatcgg gatggcgaac gacag
//
LOCUS       DASQJS010000268        19830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3307219, whole genome shotgun sequence.
ACCESSION   DASQJS010000268 DASQJS010000000
VERSION     DASQJS010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19830
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3307219"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VEW08_10775"
     CDS             complement(<1..474)
                     /locus_tag="VEW08_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYJ41269.1"
                     /translation="MPAELHETSLIALLAVGFVLALLFGLVASILKVSPIVGYLIAGI
                     CVGPFTPGFVADSGLANQLAEIGVILLMFGVGLHFSTSDLFAVRGIAIPGAIGQIVLA
                     TLMGMALTHFWGWTLGEGIVFGLCLSVASTVVLLKALEERNLVETTMGRVAVGWLI"
     gene            526..2403
                     /locus_tag="VEW08_10780"
     CDS             526..2403
                     /locus_tag="VEW08_10780"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HYJ41270.1"
                     /translation="MRASFMKAQQALGPAFIGAIGVVFGDIGTSPLYAFKESLGAAGF
                     QPGDASTVLGLLSLIFWSLAIVVSVKYLAVIMRADNHGEGGILSLVALVQQRFGTAEP
                     WARRAVALGVLGAALFYCDALITPAISVLSAVEGMEILNPNFERAVIPVTLGVIIVLF
                     AIQRRGTEKVGRMFGPIMILWFVVLAVLGALEIVRAPQVLAAVNPWYALQLFSSHPGL
                     SLVIIGAVFLVVTGAEALYADMGHFGRRPVAIAWLTLVWPALLINYFGQGAMLLQSAT
                     PIANPFYALAPQGWLPALVLLSTAATVIASQATISGAFSVTREAVQLDLLPRVRVLQT
                     SAEAHGQIYVPAANLFMFVAVVMFVVGFGSSSALSAAYGASVVGTMLITTLLGALVAG
                     SLWNWPVWRVAALFGLMLLVDLAFVAGNATKIVSGGWVPLALAGLMFAMFITWRDGRQ
                     RLRAELEYRAVSIKKLPELLMPAAKVPGTAVFLVSQSGFVPTALLRNLEHNKVHHDQI
                     VILHIEIQRIPRTDPMCRVVIEEMIPGVFDVRARFGFMETPDVGEALRNARRQGLKVF
                     AEDSSFFLGWHLVRARPQPGFPGVKSRAFAFLQRRSAQAAEFFNMPTRGVVVLATEIE
                     I"
     gene            2775..4757
                     /locus_tag="VEW08_10785"
     CDS             2775..4757
                     /locus_tag="VEW08_10785"
                     /inference="COORDINATES: protein motif:HMM:NF015054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HYJ41271.1"
                     /translation="MRIDLLRGMILCVFAFLTQGAFAHGLVQDPPARNWFCGAVTKPD
                     HVQNGVAQYPVCGGAFFAPGIEPTAGYSFMSVLTHTTGRAGVGTRTNVCGFNSETWNG
                     AATVWDQAIDWPTVPMTAGPRNFTWNISWGPHFSDSSDFRYWITKPGFVWQTGRPLTF
                     ADFEDAPFCDLAYNDATPNANPNLVPDKANAMFLTRCTVPSRSGRHVIYAEWGRTPPT
                     FERFHGCIDAQFSGSPPPTVTANIVTTPANVATFTGSGSIALNGSSSVGSNLSYTWSV
                     DSENPSLYSITNPMSAQATLNLGVPQASGNVTVNLLVTSGNSSDTETRTILHQPAVSN
                     QWFDLGVLTADARTLVVGDRVNVRTVSTTGQDAFWPTTPITITSANTGATQWPVTLAN
                     AVNALNGNVRIGVLNAQNQVVPAANATSNRVYSLTSANIASAFLQVVIGAVPTAPTGL
                     AGTPGNAQVALTWNAVSGATAYNVKRSTTNGGPYANVAQNVTGTAYTNTGLTNGTPYY
                     YVVTAVNSSGESPVSLQVSATPAATGGETGGVTVTKAVTSASPWFNEIQVRLANTSSI
                     TAMTVTVVVQRTPGVSYSGAYNTVGGQISQANASTTSTVTYTWTLNAGQQLGASTSRT
                     FAAQFGGNGTAHPVTGDTWTVNYTTGGVARSQTGSF"
     gene            4878..7187
                     /locus_tag="VEW08_10790"
     CDS             4878..7187
                     /locus_tag="VEW08_10790"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HYJ41272.1"
                     /translation="MRIHTKEMEFMRIKKHRLAWTAAAACAWALATGVASAQTPACQV
                     TYTKSWEGGNGFGANVVITNNGPAITNGWTLVFNFPNGQRLQNGWPVSFTQPAGSATL
                     TIASNADWNRSIGSGGSFTAGFNGTFTGTNNIPTAFTLNGTSCNGGTTPTNSPPNVSL
                     TSPTSGQQIANGATIPLAATASDNGSVSRVEFRVDGALVSTDTSSPYGFTNPALAAGS
                     HTAQATAFDNASPPLSTSTALIPFTVLGSTSPRTIVVNPATLNLASGGTGTSNVRLGQ
                     APTGNVVVTLTRSGSTAISSSASSVTLTTGNWSTGVNVTFTAAAGTTAATSTFAAAAT
                     NYTGASITVNRTATNPNPGRVDNPYSGAGVYNNPIWRANAVAGGGSSIANQPTAVWMD
                     RISAIAGNNSGSTGAWGLVQHLNEAVTQDSANGSTPLVFQLVIYNLPGRDCAALASNG
                     ELGPNDLPRYKAEYIDVIAAILRRSEYAGLRIATIIEIDSLPNLVTNVTSRPTGTAQC
                     DVMFQNRGYVDGVGYALATLGAISNVYNYIDAGHHGWLGWDTNFAPSVQMMLQAANAS
                     GSTPANVVGFITNTANTSALQEPYITVDSTTRPSTWIDWNQFNDELTYAQAFRNQAVS
                     AGFPSNIGMLIDTSRNGWGGSARPTGPSTLADLNARINASRIDRRIHKGNWCNPSGAG
                     IGERPRAAPATGIDAYVWVKPPGESDGSSTLIPNDEGKGFDRMCDPTYGGNPRNNGNM
                     TGALPNAPLSGHWFQAQFQQLLQNAYPPL"
     gene            7543..9366
                     /gene="msrA"
                     /locus_tag="VEW08_10795"
     CDS             7543..9366
                     /gene="msrA"
                     /locus_tag="VEW08_10795"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HYJ41273.1"
                     /translation="MTRNLSLALLIGVLSVNACGSEAAVKIPAPAFDQPAAPGKLQTA
                     VVAGGCFWGVQGVYQHLKGVKSVLSGYAGGDGSRADYESVSAGDSGHAESVQIVYDPA
                     QVTYGQILHVFFSVAHDPTQLNRQGPDTGTQYRSAIFYTSDEQKKIAEGYIAQLDKTG
                     AYPRRIVTRVDALKRFYEAEGYHQDYLLNHPTNPYIVINDLPKVRNFEHTLPALYVAK
                     PVTVSASTREGAQAAPKATTKAAPAPANSGAMMMAAAEKPAEKSTQKPAQKSTEGGAM
                     MSAAGASGPSKTEGPLPNLDGATGWLNSRPLTPESLRGKVVVVDFWAYSCINCLRTMP
                     YVNAWYRHYKDSGLVILGVHSPEFAFEKDIANVREAVKKFDIQYPVALDNEMAIWKAF
                     NNRFWPAHYFVDAKGQIRGHHFGEGKYGKSEREIRRLLTEAGAKNLPDPLDAAAGVGV
                     AAPADTANVASPETYVGYARAENFVSPGAFARDAKKTYAIPKTLTLNQWALGGPWQVA
                     REHARLGASPGRIAFRFKARDLHLVLGPATPGKPVRFRVTIDGAPPKADAGMDVDADG
                     NGVVREHRLYQLVREKGPVSEQEFVIEFLDPGVDAYSFTFG"
     gene            9392..9946
                     /gene="msrB"
                     /locus_tag="VEW08_10800"
     CDS             9392..9946
                     /gene="msrB"
                     /locus_tag="VEW08_10800"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HYJ41274.1"
                     /translation="MRSRRGFLLGALVAPVALAVGACARGSDKPRSATVEIESFSADG
                     KSLGKASVARVIKSEEQWRSQLSSDSYVVTRKAGTERPFSGKYNKNHDDGLYRCICCD
                     TALFDSKTKFESGTGWPSFWKPISSANVTESGYSSLTQGTEVNCKLCDAHLGHVFDDG
                     PDPTGLRYCMNSVALNFVGRAKST"
     gene            complement(9950..12025)
                     /locus_tag="VEW08_10805"
     CDS             complement(9950..12025)
                     /locus_tag="VEW08_10805"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HYJ41275.1"
                     /translation="MKRVYIVVAMLARAGMANAADADWPRYGGDDAKTRHSPLAQINR
                     GNVGQLMVAWQHDTGEKGDTQTQPIVVGRTLYGYTPSHKAFALEATTGRQLWLFDSGI
                     SGSGANRGLMHWTDGKEARVFAAVDNFVYALNADTGKPVEGFGRNGRVDLRENLGRDP
                     ASQSVRLTSPGVIYKELMILGGRVSETLPASPGHVRAYDVKTGALRWSFHTIPQPGEP
                     GHDTWPGDAWQYIGGANSWPGMTLDTRRGLVFVPTGSAAADFYGGNRRGDNLYANCLL
                     ALDAATGKLRWHFQFVKHDVLDRDLPTAPTLVTLRIKGKSVDALAQTTKQGFVFVLNR
                     DTGKPVFPVEEVPAPPSDVPGEVAYPTYLRPKLPAPYARQRLTADSLTTRTREAAAWA
                     REQFATVRSDGPFKPLAVGIDTTIYPGFDGGAEWGGPAFDPDTGLLYVNSNEMAWLGS
                     LAVNDTGRSTGKSIYLRDCAACHGDNLQGSPPQFPSLVGIAGRMTPADFTVLVRKGGG
                     RMPAFAGMQGEAISAIAKYLFDGEDIPAGAPDLSPMNQEYRFTGYRRWLDPAGYPAVA
                     MPWGTLNAIELATGKQVWSVPFGEFPELAAKGLRDTGSENYGGPLVTSGGLLFIGATI
                     HDRKFRAYDKANGKLLWETTLPWSADATPITYQVDGRQYVAIFASGGKERFGPGSGTY
                     LSFALPARP"
     gene            12200..13378
                     /locus_tag="VEW08_10810"
     CDS             12200..13378
                     /locus_tag="VEW08_10810"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HYJ41276.1"
                     /translation="MTRKRKTVIVLLAVALVLIAARVAMPYYVENYANDKLASLDSYD
                     GHVGDIDIHLWRGAYSIDDIEIVKTGASRPVPFFRSDRVNFSIEWRSLFRGSIVSEAS
                     FLSPELNLVQGRSARDSQLGKEENWNARLEELFPFRFNTIQVRNGTVRFLAPGINTRD
                     AITARHVTGDISNLTNVLDSGKETFADFHVKADVLDGAPATVEGSANAFASQPTFDVN
                     LEVKKVQLPQVNPWLREYIKADAEAGKFELYMELAAADGKFTGYAKPILQDVNIYRSG
                     EEEQSPLKRLWEGFLDFAANVFENQEADQVAARIPFSGTLRDPETNLFATIASVMRNA
                     FISAFARSLEGSITLRDVKKNLGEIDPNKESEPDKKSENKNAQQKDDAKRPAGPAGKV
                     "
     gene            complement(13391..14077)
                     /locus_tag="VEW08_10815"
     CDS             complement(13391..14077)
                     /locus_tag="VEW08_10815"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HYJ41277.1"
                     /translation="MQKKRAGPTLGALLRRLRHREGWTLKEMSAKSGIPFSTLSKIEH
                     DRLTLSFDKLQQLSHRLGLRMSELFAEDTGEHGQAVTARRSLGDVDKAVRVTTPNYDY
                     YYLCTELRRKRMIPVITKIRAASREEFGELVRHAGEEFIYVLKGRIVVHTEFYDPVTL
                     NERQSIYIDSGMGHAYLTAAGCHEAEVLGVMSSADDDQLMQSLLTLHEEQSQKTSAAP
                     NDTADVARRR"
     gene            14206..16209
                     /locus_tag="VEW08_10820"
     CDS             14206..16209
                     /locus_tag="VEW08_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYJ41278.1"
                     /translation="MSKLKALILVAAGFSSAAFADEPSVPLSPAPTTASAAAPANSPD
                     QAGGAVPLTKSDVDTWLDGFLPYALRTADIPGAVVTVVKDGQLLTARGYGFADREKRT
                     PVDPDRTLFRPGSVSKLVTWTAAMQLVEQGKLDLDKDVNTYLDFKIPEYDGKPVTLRQ
                     IMTHTAGFEEAVKDIIFTDPAHLLPLGEYLKRWTPKRVFEPGTTPAYSNWATALTGYL
                     VERVSGESFDDYCDKHIFAPLGMTNSTMRQPLPAALAGQMASGYKPGQEAGKFEIIGP
                     APAGSLSATGTDMAKFMIANLDGGKGLLSPEVAATMYNPPLDKFNPVSLLPPLNRMRL
                     GYFETNVNGREVIGHLGDTEFFHTSLHLLMKEGVGIYFSFNSGGREGQVQTLRWSLFE
                     DFADRYFPATAAADAPLDAKTAAEHAAMMAGNWQVSRTAWSNPIAVLNLIGQTKVAVG
                     PKGGLLIPDLLGANKLPREWEEIAPFVWREKGGHDLLAAQVKEGQVVRWSFGLLAPFM
                     VFDRVPAHRSSSWITPVASISLGVLLLTVLFWPIGWFARRKYRATFPLAGTALRAYKW
                     TRWASLAVVLVLVGWMAMIGALFANLENLAGAFDALLWVLQIVGLVAFIGAVAIAGWN
                     AWLTWRDGRRWTAKTWNTLIAVSSVVLLYVAFNFNLLSMTVNY"
     gene            16209..17219
                     /locus_tag="VEW08_10825"
     CDS             16209..17219
                     /locus_tag="VEW08_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HYJ41279.1"
                     /translation="MSRLKLDVAVETLRLARPFKISGYCFETADVVYVTLDDGTHRGR
                     GEAAGVYYMQDDLAHMAKALQAARAEIEAGPTRHELRHILPPGGARAAVDAALWELEA
                     LRAGTTAWRIAGLTSVHPVRTTLTVSADTVEAMAARAVTYANAKAIKVKLTGELDLDL
                     ARVRAIRAARPDVWLGVDANQGYSIGELPALVRGLCEHRVLLLEQPLPRGREADLQGF
                     DSVIPIAADESVLSLAELPRAVGSFDVVNIKLDKCGGLTEGLMIAEEARRLGLEVMVG
                     TMIGTSLATAPAFILAQQCSIVDLDGPTFLVKDRSPAVVYRDGDLFAGDDVWGAASSV
                     AA"
     gene            17233..18516
                     /locus_tag="VEW08_10830"
     CDS             17233..18516
                     /locus_tag="VEW08_10830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HYJ41280.1"
                     /translation="MTSEKTWFGHPRGLTVLFFTNMWEQFSYYGMRAILVYYMTKQLL
                     LAQETSSVIYGIYTGCAYFTPLIGGFIADRFLGKKRAIIIGGSVMALGHFMMTAEPLF
                     YVALATIAIGNGLFLPSLPSQIRDLYQPGDPRAGRAYNVYYVGVNIGGFLAMLVCGAL
                     GEAFGWHWGFGAAGVGMVTGLLFYLWGQRYLPAQTQLPRAELLAAPRENMARDTLLLL
                     FSLGVAVAVFRSAYEQVGNTVALWFDTGVYRLADGFSIPFTWFFAINPLCVMLITPFL
                     LAWWRKREAAGHTPLPTQRMATGALIVGASYLFLAALSWTGDSGTVHWQWFALFWVVL
                     TFGELFILPTGIGLFARLAPQRLGATTVASWFLATFAGNLAAGLVGTFWSRMSPPAFF
                     VLLAGICVLAATALRLLDARAQHVTTITRPQANPD"
     gene            18623..>19830
                     /locus_tag="VEW08_10835"
     CDS             18623..>19830
                     /locus_tag="VEW08_10835"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYJ41281.1"
                     /translation="MTTRTRIFRSTGALSALLMMQSAWAQDRPDVTNLDEVLVTAQKV
                     SQSVIDVPLAISVLGSDDLERTQSLNFQDYTKLVPGLQLTQASPGFGRLVIRGINTGG
                     SASTVGVYVDETPFGSSSGIANGAILAGDFDTFDVARVEVMRGPQGTVYGASSLGGVL
                     KYVTNEPQFDSFEGRVRGSLETVDDGDSSWSGAAAANIPVSDSVAIRASGFYRNVGGF
                     IDSIGTGGSDVADDINGSTVAGGRASMLFKPSEAFSLQLTAFIQDIANDASSVVDSDP
                     MTGESLYGGLTQSQFVPESADVSYRVYNATAIWDLGFGDLTSSTSYGSNTQDFRVDFT
                     TLLRNAFGLDIFDSYNQQTTRVKRFTQELRLASHENEKVDWLIGAYYNDEDGLIYQNV
                     HVVEPGTLND"
BASE COUNT         3670 a         6274 c         6230 g         3656 t
ORIGIN      
        1 gatcagccag ccgaccgcga cccggcccat cgtcgtctcg acgagattgc gctcctcgag
       61 cgccttcagc agcaccacgg tgctcgcgac cgagaggcag aggccgaaga caatgccttc
      121 accaagcgtc cagccccaga aatgcgtcag cgccatgccc atcagcgtgg ccagcacgat
      181 ctggccgatg gcgcccggaa tggcgatgcc gcgcacggcg aacaggtccg aggtggagaa
      241 atgcaggccg acgccgaaca tcagcaggat cacgccgatt tccgcgagct ggttcgcaag
      301 gcccgagtcc gccacgaacc ctggcgtgaa ggggccgacg cagatgccgg cgatcaggta
      361 gccgacgatg ggcgacacct tgaggatgct ggccaccaat ccgaacaaca atgcaagtac
      421 gaaaccaacc gccagcagtg cgatgagcga agtttcgtgt aattccgcgg gcatacgcgc
      481 gagggtaccc gaaaaagtgc gaattgcggc gctgcggggc ctagaatgcg cgcctcgttt
      541 atgaaggctc agcaagctct cggacccgca ttcatcggcg ccatcggcgt cgtgttcgga
      601 gacatcggta ccagtccgct ctacgcgttc aaggaatcgc tgggcgccgc gggatttcag
      661 ccgggcgacg catccacggt gttagggctg ctgtcgctga tcttctggag cctggcgatc
      721 gtcgtcagcg tgaaatacct cgccgtgatc atgcgcgccg acaatcacgg cgaaggcggc
      781 atcctctcgc tcgtcgcact cgtgcagcag cgcttcggca ccgccgagcc ctgggcgcgc
      841 cgcgcggtgg cgctcggcgt actcggtgcc gcactctttt attgcgacgc gttgatcaca
      901 cccgccatct ccgtcttgag cgccgtcgaa ggcatggaaa tcctgaatcc gaatttcgag
      961 cgcgccgtca tcccggtgac gctcggcgtc atcatcgtgt tgtttgcgat tcaacggcgg
     1021 ggcaccgaga aagtgggccg gatgttcggt cccatcatga ttctctggtt cgtggtgctc
     1081 gcggtgctgg gcgcgctgga gatcgtgcgt gcccctcagg tgctcgcggc agtgaatcct
     1141 tggtatgcgc tgcagttgtt ctcgtcgcac cctgggctgt cgctggtgat catcggcgcc
     1201 gtattcctgg tggtgacggg cgccgaagcg ctgtacgccg acatgggaca tttcggccgg
     1261 aggcccgtcg cgatcgcctg gttgacgctg gtttggccgg cgctgctcat caactacttc
     1321 ggccagggtg caatgctgtt gcagtcggcc acaccaatag ccaacccgtt ctatgcgctg
     1381 gcacctcaag gatggctgcc ggcactcgtg ctgctgtcca cggccgcgac agtcatcgcg
     1441 tcgcaggcga caatttccgg cgccttctcc gtgacccgtg aagccgtaca gctcgacctg
     1501 ttgccgcgcg tgcgtgtgct gcaaacctcg gcggaggcgc atgggcagat ctacgtcccg
     1561 gccgcgaacc tgttcatgtt cgtcgccgtg gtgatgttcg tagtgggttt cggcagctcg
     1621 agcgcgctgt cggccgcata tggtgcctcg gtggtcggca ccatgctcat caccacttta
     1681 ttaggcgcgc tggttgccgg ctccctgtgg aattggccag tgtggcgcgt ggctgcactg
     1741 ttcggcctca tgttgctggt cgacctggca ttcgtcgccg gaaatgccac caagattgtg
     1801 agtggcggct gggtgccgct ggcgctggca ggcctgatgt tcgccatgtt catcacctgg
     1861 cgcgacggac gccagcgact tcgggcggaa ctcgaatacc gtgcggtgtc gatcaagaag
     1921 ctgcccgagc tcctgatgcc ggcggcgaaa gtgcccggca ccgcggtgtt tctcgtgagc
     1981 cagagtggtt ttgtgcccac cgccctgctg cgcaacctgg agcacaacaa ggttcaccac
     2041 gatcaaatcg tgattttgca catcgagatc cagcgcattc cacgcaccga tccgatgtgc
     2101 cgcgtggtga tcgaagagat gattccgggc gtatttgacg tgcgcgcgcg atttggcttc
     2161 atggagacac ccgatgtcgg cgaggcgctg cgcaacgcgc gacgccaggg tctcaaggtg
     2221 ttcgccgagg attcatcgtt ctttctgggc tggcacctcg tgcgcgcgcg accgcagccg
     2281 gggtttcccg gagtgaaatc gcgcgccttc gcattcctgc agcgtcgaag cgcgcaggcc
     2341 gcggagtttt tcaatatgcc gacacgaggc gtcgtggtcc tcgccacgga aatcgaaatc
     2401 tagcgagaca ttttccgggg ctgcacgttg acgtcaccag ccaacgcgtg tccgacagcc
     2461 agtacaaaaa agtcctgtga gccaccttgg cctttccggc cgtgcggcta gtcggaatcc
     2521 gccttgacaa cgctgtccaa actacccgga tgcggtgctc ccgagttgtg cacggcccga
     2581 ttacgggcct aacatcgccc ccgctcgcgc atgaagcgcg ggcattcagg gggacttgta
     2641 tggacacagg ttatctttcg cggcaggtat cgccgcacat ccgccacgcg gctccgcact
     2701 cggagaaacc gccctagctc ccgatccgtt tcgctatcga tttatttcgt acgaacgatc
     2761 acgagggagc tgtcatgagg atcgacttac ttcgagggat gattctgtgc gtgttcgcat
     2821 tcctgaccca gggcgcattc gcccatggac tggtccagga cccgccggca cgcaactggt
     2881 tctgcggtgc agtgaccaag ccggaccatg tgcaaaacgg cgtggcccaa tatccagtgt
     2941 gcggtggagc gttcttcgct ccgggcatcg aaccgaccgc cggctacagt ttcatgagtg
     3001 tgctcacgca caccaccggg cgcgcgggcg tgggcacacg caccaacgtc tgcggtttca
     3061 actccgagac ctggaatggc gccgcaacgg tttgggatca ggccatcgac tggccgacgg
     3121 tgccgatgac cgcgggtcca cgaaacttca cctggaatat ttcctgggga cctcacttct
     3181 cggacagctc ggattttcgc tactggatca cgaagccggg cttcgtgtgg cagacggggc
     3241 gtccgctgac gttcgcagat tttgaagacg cgccgttctg cgatctcgcc tacaacgatg
     3301 cgacgccgaa cgccaatccg aacctggtgc ccgacaaggc gaacgccatg ttcctcacgc
     3361 gctgcacagt gccttcgcgc tccggacgcc acgtgattta tgcggagtgg ggccgcacgc
     3421 cgcccacatt cgagcgcttc catggctgca tcgacgcgca gttctcaggc tctccgccgc
     3481 ccaccgtcac ggcgaacatc gtgacgacgc cggcgaacgt ggccacgttc accggttcgg
     3541 gctcgatcgc gctcaacggg agcagctcgg ttggcagcaa cctcagctac acctggagcg
     3601 tggattcgga aaatccgtcg ctgtacagca tcaccaatcc gatgagcgcg caggccacgc
     3661 tgaatctcgg cgtgccgcag gcctcgggta acgtcacagt caacctgctc gtgaccagcg
     3721 gcaactcgtc cgacaccgag acgcgcacga tcctgcacca gccggccgtg agcaatcagt
     3781 ggttcgacct cggcgtgctg accgcggatg cgcgcacgct cgtcgtgggc gaccgcgtca
     3841 acgtacgtac cgtgtctacc acgggtcagg atgcgttctg gccgacgacg cccatcacca
     3901 tcacgtccgc gaacaccggt gccacgcaat ggccggtgac actcgcgaat gcggtgaatg
     3961 cgctcaacgg caacgttcgc atcggcgtgc tgaatgcgca gaaccaggtg gttccggcgg
     4021 ccaatgcgac cagcaaccgc gtttactcgc tgaccagcgc gaacatcgcc agtgccttcc
     4081 tgcaggtggt gatcggagcg gtaccgaccg cgccgaccgg tcttgcgggt acgccaggca
     4141 acgcgcaggt ggcgctcacc tggaacgcgg tgtccggtgc cacggcctac aacgtgaaac
     4201 gctcgacgac caatggcggt ccttacgcca acgtcgcgca gaacgtcacg ggtaccgcgt
     4261 acaccaacac cggcctcacc aatggcacgc cttactacta cgtcgtgacg gcagtgaact
     4321 cgagcggcga gagccccgtg tcgctgcagg tgagtgcgac cccggccgcg accggtggcg
     4381 aaaccggtgg tgtaacggtg acgaaagcgg tgaccagcgc cagcccgtgg ttcaacgaga
     4441 tccaggtccg tctcgccaac accagctcca tcacggccat gaccgtgacg gtggtggtgc
     4501 agcggacgcc cggcgtcagt tacagcgggg cgtacaacac ggtgggcgga cagatctcgc
     4561 aggccaacgc gagcacgacc tcgaccgtga cgtacacatg gacgctgaac gcgggtcagc
     4621 agctcggcgc gtcgacttca cgaaccttcg cggcgcagtt tggcggaaac ggaactgcgc
     4681 acccggtaac cggcgacacc tggacggtga actacaccac cggcggcgtc gcgcgcagcc
     4741 agacggggag tttctgatcc gccaggcctg aaagaagcat gcatggacgg cgctcaccgc
     4801 aggaacggtg agcgccggcg cattgaccct gaaagagcac ggacgagcgg cgttcaccgc
     4861 ggggagcgga gggcgccgtg cgcatacata ccaaggagat ggaattcatg aggatcaaga
     4921 aacaccgcct cgcgtggacg gcggccgccg cctgcgcgtg ggcgctagct acaggcgtcg
     4981 catcggcgca aacccctgca tgtcaggtca cgtacacaaa atcgtgggaa ggcggtaatg
     5041 gcttcggcgc caatgtcgtc atcaccaaca acggtccggc catcaccaac ggctggacgc
     5101 tggtcttcaa ctttcccaac gggcagcgat tgcagaatgg ctggccggtt tccttcactc
     5161 agcccgcggg cagcgcgacg ctgacgatcg ccagcaacgc cgactggaac cggagcatcg
     5221 gcagtggtgg cagcttcacc gcgggattca atggcacgtt caccggcacc aacaacatac
     5281 cgacggcatt cacgctgaat ggcaccagct gcaacggcgg caccacgccg accaattcac
     5341 cgcccaacgt gagcctgacg agcccgacga gcggccagca gattgcgaat ggcgcgacga
     5401 tcccgctcgc ggccaccgcc agcgacaatg gctcggtgag ccgagtggag ttccgcgtcg
     5461 acggcgctct cgtgagcacc gacaccagct cgccctatgg cttcacgaac ccggcactcg
     5521 cggccggcag tcacacggca caggccacgg cgttcgacaa cgcatcgcca ccgctgtcga
     5581 cttccacggc tttgattccg ttcacggtgt tgggcagcac ttcgccgcgc accatcgtcg
     5641 tcaatcctgc gacgctgaat ctggcgagtg gcggtactgg cacgtcgaac gtccggctgg
     5701 gacaggcacc cacgggcaac gtggtggtga cgctgacgcg cagcggcagc accgccatca
     5761 gctcgagcgc gagctcggtc acgctgacca ccggcaactg gtcgaccggc gtgaacgtga
     5821 cgttcacggc ggcggccggc acgacggcgg cgacatccac tttcgcggcc gcggcgacta
     5881 actacaccgg cgcgtcgatc accgtgaatc gcaccgccac gaacccgaac ccgggtcgcg
     5941 tcgacaatcc gtactcgggc gcgggcgtgt acaacaaccc gatctggcgc gcgaacgcgg
     6001 tggccggtgg cggcagctcc atagccaacc agccgacggc ggtgtggatg gatcgcatca
     6061 gcgcgattgc cggcaacaac agcggcagca ctggcgcctg gggtctggtg cagcacctga
     6121 acgaggcggt cacgcaggac tcggccaacg gttcgacgcc gctggtcttc cagctggtga
     6181 tctacaacct gccgggccgc gactgcgcgg cgctcgccag caacggtgag ctcggaccca
     6241 acgacctgcc gcgctacaag gcggagtaca tcgatgtcat cgccgcgatc ctgcggcggt
     6301 cggagtacgc cggactgcgc attgccacca tcatcgagat cgattcactt cccaacctgg
     6361 tcacgaatgt gacgagcagg ccgaccggca ccgcacagtg cgacgtgatg ttccagaacc
     6421 gcggttatgt cgatggcgtg ggctacgcgc tcgcaacgct gggtgccatt tccaacgtct
     6481 ataactacat cgacgcgggt caccatggct ggctgggttg ggacaccaac ttcgcgccat
     6541 ccgtgcagat gatgctgcag gcagccaatg cctcgggcag cacgcctgcc aacgtggtgg
     6601 gcttcatcac caacaccgcg aacacctcgg cgttgcagga gccgtacatc acagtggact
     6661 cgaccacgcg gccgtcgacc tggatcgact ggaatcagtt caacgacgag ttgacctacg
     6721 cccaggcgtt ccgcaaccag gcggtgagcg caggcttccc gtccaacatc ggcatgctca
     6781 tcgatacctc gcgcaatggc tggggcggtt cggcgcggcc gacgggtcct tcgacgctcg
     6841 cggacctcaa cgcgcgcatc aacgcgtcac gcatcgaccg gcgcattcac aagggcaact
     6901 ggtgcaatcc gagcggcgct ggcattggcg aacgtccgcg tgcggcacct gccaccggca
     6961 tcgatgccta cgtgtgggtg aagccgccag gcgaatcgga cggctccagc accctcatcc
     7021 cgaacgacga gggcaagggc ttcgatcgca tgtgcgatcc gacctacggt ggcaatccgc
     7081 gcaacaacgg caacatgacc ggagcgctcc cgaacgcccc attgtcgggg cattggttcc
     7141 aggcgcagtt ccagcagttg ctccagaacg cttacccacc gctgtaggtg gctttaaggc
     7201 ttgcggttca gcgaccctcc cagctgaacc gcaagtcttg gcgggtaaca ggcgccagca
     7261 aaggattgcc ggcgtctgtt actcgacatt ttcggttcag ttcaggcgaa gcttcacgct
     7321 ccccgctatc ggcacccgag cctgctcacg cggatcactt tcgggtcccg aaccaatacc
     7381 tttgaatcgt cgccccccgg gaattgttgc gtttgccggc ggtcatcacg caggcatacg
     7441 cggggattgc gccctgcatg ccagaatccc cggcgggcga aataatgcag cggaaaggcc
     7501 ggtctgatca gttacccagg catcggcccc acaggaaccc caatgacgag aaatctgtcc
     7561 cttgcgctcc tgatcggcgt gttgagtgtc aacgcctgcg gctctgaagc cgcggtaaag
     7621 atcccggcgc cggcattcga ccaaccggct gcgcccggga agctgcagac cgccgtggtg
     7681 gctggcggat gcttctgggg agtgcagggc gtctatcaac atctgaaagg tgtgaaaagc
     7741 gtgctctccg gttacgccgg aggcgatggc tcgcgcgcgg actatgaatc ggtgagcgcc
     7801 ggagattcgg gccacgccga atcggtgcag atcgtgtacg accccgcgca ggtcacctac
     7861 ggccagatcc tgcacgtgtt cttttcagtg gcgcatgacc cgacacagct gaaccgccag
     7921 ggaccagaca cgggcacgca atatcgctcg gccattttct ataccagcga cgagcagaag
     7981 aagatcgcgg aaggctacat cgcgcagctc gacaagaccg gtgcgtatcc gcggcgcatc
     8041 gtcacgcgcg tggacgcgtt gaagcgcttc tacgaagcgg agggctacca ccaggactac
     8101 ctgctcaatc acccgacgaa tccctacatc gtcatcaacg atcttccaaa ggtgcgcaac
     8161 ttcgagcaca cgctaccggc gctgtacgtc gcgaaaccgg tgaccgtgag cgcgtcgacg
     8221 cgcgagggag cccaggcggc gcccaaggcc acgaccaaag ccgcgccggc acccgcgaac
     8281 agcggcgcca tgatgatggc ggctgccgaa aagcccgccg aaaaatcaac ccaaaaaccg
     8341 gcccaaaaat caacggaggg cggcgcgatg atgtcggcag ccggcgccag cgggccgtcg
     8401 aagaccgaag ggccgctgcc caatctcgat ggggcgacgg gctggctcaa cagcaggccg
     8461 ctcacgcccg agagcctgcg cggcaaggtc gtggtcgtcg atttctgggc gtactcgtgc
     8521 atcaactgcc tgcgcacgat gccatacgtg aacgcctggt atcgccacta caaggattcg
     8581 ggtctggtca tactcggtgt gcactcaccg gagttcgcgt tcgaaaaaga catcgcgaac
     8641 gtgcgcgaag cagtcaaaaa gttcgacatc cagtaccccg tcgcgctcga caacgagatg
     8701 gcgatctgga aagccttcaa caacaggttc tggcccgcgc actatttcgt cgacgccaag
     8761 gggcagatcc gcggccatca tttcggcgag ggaaaatacg gcaagtcgga acgcgagatc
     8821 aggcggttgc tcaccgaggc cggtgcgaaa aacctgcccg acccgctcga cgccgccgcg
     8881 ggtgttggcg tcgccgcgcc ggcggacacc gcgaacgtgg cgtcaccgga gacgtacgtc
     8941 ggctacgcgc gcgctgaaaa tttcgtctcg ccgggcgcct tcgcccgcga tgcaaagaaa
     9001 acctacgcca tcccgaagac gctcacactc aaccagtggg cgctcggtgg cccctggcag
     9061 gtcgcacgcg agcacgcgcg cctgggcgct tcaccgggcc gcatcgcctt ccgcttcaag
     9121 gcgcgggacc tgcacctcgt gctgggtccg gcgacgccgg gcaagccggt gcgctttcga
     9181 gtcacgatcg acggcgcacc gccgaaggcg gatgccggca tggacgtgga tgcggacggc
     9241 aacggagtcg tgcgtgagca tcggctctat cagctcgttc gcgagaaagg gccggtgagt
     9301 gagcaggagt tcgtcatcga attcctggat ccgggcgtcg acgcctattc attcacgttc
     9361 ggttgaaacg ggagagttta tggacgaggc aatgaggtca cgacgcggat tcctgttagg
     9421 cgcgctggtg gcgcccgtgg cgctcgccgt cggggcctgc gcgcgcgggt ccgacaagcc
     9481 caggtccgcg accgtggaga tcgagagctt ttccgccgac ggcaagagcc tgggaaaggc
     9541 cagcgtggcg cgcgtgatca agagcgaaga acagtggcgc agccagctct cgtccgactc
     9601 ctatgtcgtg acgcgcaagg cgggcaccga gcgtcccttc agcggcaaat acaacaagaa
     9661 tcatgacgac gggttgtatc gctgcatctg ttgcgacacg gcgctgttcg attcgaagac
     9721 caagttcgaa tccggtaccg gctggccgag tttctggaag ccgatctcga gcgccaatgt
     9781 caccgagtcc gggtacagca gtctcaccca aggcacagaa gtgaactgca agctttgcga
     9841 tgcgcacctc gggcacgtgt tcgatgacgg gccggacccg accggcttgc gctactgcat
     9901 gaattccgtg gcgctgaatt ttgtcgggcg tgcaaaaagc acctgagcct cacggcctgg
     9961 cgggaagggc aaacgacaga taggtgccac tacccgggcc gaaacgctct ttgccgccgc
    10021 tcgcaaatat cgcgacgtac tggcgcccgt cgacctggta ggtgatgggc gtcgcgtcgg
    10081 cggaccaggg cagcgtggtt tcccagagta gtttgccgtt cgccttgtca taggcgcgga
    10141 atttccggtc gtggatggtc gcgccgatga acagcaaacc gccggaggtc accagcgggc
    10201 cgccgtagtt ttcgctgccg gtgtccctga gacccttcgc ggcgagttcg ggaaactcgc
    10261 cgaacgggac gctccagact tgttttcccg tggcgagctc gatggcattg agcgtgcccc
    10321 atggcattgc cactgccggg taccctgccg gatcgagcca gcggcgataa cccgtgaagc
    10381 ggtattcctg gttcatgggc gacaggtcgg gggcgccggc ggggatgtcc tcgccgtcga
    10441 acaggtattt tgcgatggca ctgatcgcct cgccctgcat tccggcgaac gccggcatgc
    10501 ggccgccgcc cttgcgcacg aggacggtga aatctgccgg cgtcatgcgt cctgcgatgc
    10561 cgaccagcga tggaaactgc gggggcgaac cctgcaggtt gtcgccgtga caggcagcgc
    10621 aatcgcgcag gtagattgac ttgcccgtgc tgcggccggt gtcgttgacg gcgagcgagc
    10681 ccagccaggc catttcattg gagttcacgt acagcagccc ggtgtccggg tcgaaggccg
    10741 ggccacccca ttcggcaccg ccatcgaaac ccgggtagat agtggtgtcg ataccgacgg
    10801 cgagcggttt gaacggaccg tcgctgcgca cggttgcgaa ctgctctcgt gcccaggcgg
    10861 ccgcttccct cgtgcgcgtg gtgaggctgt ctgccgtcaa ccgctggcgc gcatacggtg
    10921 cgggcagctt cggccgcagg taggtggggt aggccacctc gccgggcacg tccgacggcg
    10981 gtgcgggcac ttcttcaacc ggaaacaccg gcttgccggt atcgcggttc agcacgaaca
    11041 cgaagccctg cttggtggtt tgcgccagcg cgtctacgga ttttcccttg atgcgcaacg
    11101 tcacgagcgt gggcgcggtg ggcagatcac gatccagcac gtcgtgcttc acgaactgga
    11161 aatgccagcg caacttgccg gtggcggcat cgagtgccag caggcagttc gcatacaggt
    11221 tgtcgccgcg tcgattgccg ccgtagaaat ccgccgcggc cgaaccggtc ggaacgaaca
    11281 ccaggccgcg ccgtgtatcg agcgtcatgc ccggccaact gttggcaccg ccgatgtatt
    11341 gccacgcgtc tcccggccag gtgtcgtggc cgggctcgcc cggttgcgga atggtgtgga
    11401 agctccagcg caacgcgccg gttttcacgt catacgcccg aacgtgtccg ggagatgcgg
    11461 gcagagtctc gctcacgcgg ccgccgagga tcatgagttc cttgtagatg acaccgggcg
    11521 acgtgagacg caccgactgc gaggcgggat cgcgtccgag gttctcgcgc aggtcgacgc
    11581 gcccgttcct gccaaaacct tcgacgggtt tgccggtgtc ggcattcagc gcgtacacga
    11641 aattgtcgac cgccgcgaag acgcgcgctt ccttgccgtc cgtccagtgc atgagaccgc
    11701 gatttgcacc gctgcccgaa atcccggaat cgaacaacca caactgacgg ccggtcgtgg
    11761 cctccagcgc gaacgccttg tgcgacggcg tgtacccgta cagcgtgcgg cctacgacaa
    11821 taggctgtgt ctgcgtatcg cccttctcgc cagtgtcgtg ttgccatgca accatgagct
    11881 ggccgacatt gccgcgattg atctgcgcga gtggggaatg gcgcgtcttc gcatcgtcac
    11941 cgccataacg cggccagtcg gcgtcggcgg cgttcgccat gcctgcacgg gcaagcatgg
    12001 cgaccacgat gtacacgcgc ttcatccgga ccccttcttg taagaatcgt gtgacgcaat
    12061 ctacggcaga ttgccgcttg ggtcaccgtg cgccggcagt cacaatgcag ccgtgaatcc
    12121 caacgtgctc ctcccagcgc gcatacggac tcacaacagg gggccgcttc gtgcggcctc
    12181 ttttttttgg gcgcagtgca tgacccgcaa acgcaagaca gtcatcgtgt tgttagccgt
    12241 ggcgttggtg ctgatcgccg cacgcgtggc aatgccttat tacgtcgaga actacgccaa
    12301 cgacaagctg gcgtccctgg attcgtatga cggccatgtc ggcgacatcg acattcatct
    12361 ctggcgcggc gcatacagca tcgacgacat cgagatcgtg aagactggcg catcgcgccc
    12421 cgtgcccttc ttccgttcgg atcgcgtgaa cttttcgatc gaatggcgca gcctgtttcg
    12481 tggctcgatc gtttccgagg cgtcgttcct gagtcccgag ctcaacctgg tgcagggcag
    12541 gagcgccagg gattcgcagc tcggtaagga agaaaactgg aatgcccgac tcgaggagct
    12601 gtttccattc cgtttcaaca ccatccaggt acggaatggc accgtacgat tcctcgcgcc
    12661 cggcatcaat actcgcgacg ccatcaccgc gcgacatgtc actggtgaca tttccaacct
    12721 caccaacgtt ctcgactcgg gcaaggagac tttcgccgac ttccatgtga aggccgatgt
    12781 cctggatggg gcgccggcga ccgtggaggg cagcgccaat gctttcgcat cccagccgac
    12841 cttcgatgtg aatctcgaag tgaaaaaagt gcagctgccg caggtgaatc cgtggctgcg
    12901 cgaatacatc aaggccgacg cggaagccgg aaagttcgag ctgtacatgg agctggccgc
    12961 ggccgatgga aaattcaccg gctatgccaa gccgattctc caggacgtga acatctatcg
    13021 ctccggtgaa gaagagcaga gcccactcaa gcgcctctgg gaggggttcc tcgatttcgc
    13081 ggcgaatgtg ttcgagaacc aggaagcgga tcaggtggcg gcgcgcattc cgttctcagg
    13141 caccctcagg gatccggaaa cgaacctgtt tgccaccatt gccagcgtca tgcgcaatgc
    13201 gttcatcagc gctttcgctc gttcactgga gggttcgatc acgctgcgcg atgtgaaaaa
    13261 gaatctgggg gaaatcgatc cgaacaagga atccgaaccc gacaagaaat cggagaacaa
    13321 gaacgctcag caaaaggatg acgcaaagcg acccgccggg cctgctggca aggtgtaacg
    13381 ggccggaccc tcaacgccgt cgagcgacgt ccgcggtgtc gtttggcgcc gccgaagttt
    13441 tctggctttg ctcctcgtgc agtgtcagca gcgattgcat gagctgatcg tcgtcggcgc
    13501 tcgacatgac gccgagcacc tcagcttcat ggcaacccgc ggccgtcagg taggcgtgtc
    13561 ccattccaga atcgatgtag atcgactgac gctcgttcaa ggtcaccggg tcgtagaact
    13621 cggtgtgaac gacgatgcgg cctttgagca cgtagatgaa ttcttcgccg gcgtgccgga
    13681 ccagctcgcc gaattcttct cgcgatgccg cccggatctt cgtgatgacc ggaatcatcc
    13741 gcttgcgccg cagctcggtg cacaaatagt agtagtcata atttggcgtc gtgacgcgca
    13801 ccgccttgtc gacatcgccg aggctgcgtc gggcagtcac cgcttgcccg tgctcgccgg
    13861 tgtcctcggc aaagagctcc gacatgcgca ggccaagtcg atggctcagc tgctggagct
    13921 tgtcgaagct cagcgtcagg cgatcgtgct cgatcttgga aagcgtcgaa aagggtattc
    13981 cgctcttcgc gctcatctct ttgagtgtcc aaccttcgcg atgacgcaac cgccggagga
    14041 gggcccccag ggtcggaccg gcacgtttct tttgcacggg cgatatatct ccacatccat
    14101 tgaactgttt tctgtctagg ataataatgt cccaatccgg aacattgtgc tcgaagtcgc
    14161 ttcgaacacg cgaaaacgcg tgactataaa actatgggtg gggccatgtc taaattgaag
    14221 gcgctgatat tggtggcggc cggcttttcc tcggcggcat ttgcggatga accgagtgtg
    14281 ccgctttcgc cggcgccaac tacggcatcc gcagccgcgc cggcgaattc gccagaccag
    14341 gctggaggcg cggtaccact caccaaatcc gacgtagaca cctggctcga tggcttcctt
    14401 ccttatgcac tgcgcaccgc tgacattccg ggcgccgtcg tcaccgtggt gaaggatggg
    14461 cagctcctga cggcgcgtgg atatggattc gccgatcgcg aaaagcgcac tcccgtcgat
    14521 cctgatcgca cgctcttccg gcccggctcg gtcagcaagc tggtcacgtg gacggcagcc
    14581 atgcagctcg tcgagcaagg caagctggac ctggataaag acgtcaacac gtatctcgat
    14641 ttcaagattc cggaatacga cggcaagccc gtcacgctgc gccagatcat gacccatacg
    14701 gcggggttcg aagaggcggt caaggacatc atctttaccg atccagcgca cctgttgcct
    14761 ctcggtgaat atctgaagcg ctggacaccg aagcgtgtgt tcgaacctgg aacgacgccc
    14821 gcgtattcga actgggcaac ggcgctcacc ggctacctgg tcgaacgagt ctcgggcgaa
    14881 tcgttcgatg actattgcga caaacatatt tttgcgccgc ttggcatgac caactccacc
    14941 atgcggcaac cgttacccgc ggcactcgcg ggtcagatgg cgtcgggcta caagccgggc
    15001 caggaggccg gcaagttcga gatcatcggt cccgcgccgg ccggttcgct ctctgccact
    15061 ggcaccgaca tggcgaagtt catgattgcg aatctcgacg gcggcaaggg gctgctgtcg
    15121 cccgaggtcg cggcgacgat gtacaacccg ccgctcgaca agttcaaccc cgtgtcgctg
    15181 ttgccgccac tcaaccgcat gcggctgggg tacttcgaga ccaacgtcaa tggccgcgaa
    15241 gtgatcggcc atctgggcga caccgagttc ttccacacct cgctgcatct cctgatgaag
    15301 gaaggcgtcg gcatctactt ctcattcaac agcggaggaa gggaggggca ggtccagaca
    15361 ctgcgttggt cgctgttcga ggacttcgcg gatcgctatt tccccgcaac cgcggcggca
    15421 gatgcaccac tggatgcgaa aacggccgct gaacatgccg ccatgatggc cggcaactgg
    15481 caggtgagcc gcacggcctg gagcaatccg atcgccgtgc tgaacctcat cggccagacc
    15541 aaggtggctg tcggaccgaa aggcggattg ctgatcccgg atctgctcgg cgcgaacaag
    15601 ctgccgcgtg agtgggaaga gatcgcgccg tttgtctggc gcgagaaggg cgggcacgac
    15661 cttctcgccg cacaagtcaa agagggccag gtggtgcgct ggagcttcgg cctgcttgcg
    15721 cccttcatgg ttttcgatcg cgtcccggcg catcgttcct cgagctggat cacgccggtt
    15781 gcttccatca gcctcggcgt tctgttgctg acggttctgt tctggcccat cggctggttc
    15841 gcgcggcgca agtaccgcgc gacattcccg ctcgccggca ctgcgctgcg ggcttacaag
    15901 tggacgcgtt gggcatcgct ggccgtggtg ctcgtcctcg tcggctggat ggcgatgatc
    15961 ggcgcgctgt tcgccaatct cgagaacctg gcgggcgcct tcgacgcgct gctgtgggtg
    16021 ctgcaaatcg tcggcctggt tgcattcatc ggcgccgtag cgatagcggg atggaacgcc
    16081 tggttgacct ggcgcgatgg tcgccgatgg acggcgaaga cctggaacac gctgatcgcg
    16141 gtttcttcgg tcgtgctgct gtacgtcgca ttcaacttca atctcctctc gatgacggtg
    16201 aactactgat gtcgaggctg aagctcgacg ttgccgtcga aacgctcagg ctggccaggc
    16261 cgttcaagat ctccggttat tgcttcgaaa cggccgatgt cgtgtacgtc acgctggatg
    16321 acggcacgca tcgtggccgt ggcgaggcgg cgggcgtcta ctacatgcag gatgacctcg
    16381 ctcatatggc gaaggcgctg caggcggcga gggcggagat cgaggcaggc ccgacgcgcc
    16441 acgaactgcg ccacatcttg ccgcccgggg gcgcgcgcgc cgcggtcgat gccgcgttgt
    16501 gggagctaga agcgctgcgt gccggcacta ccgcctggcg gatcgccggg ctgaccagcg
    16561 tgcatcccgt gcgcacgaca ctcacggtga gtgcggatac ggtcgaggcc atggctgccc
    16621 gcgctgtgac gtacgccaat gccaaagcga tcaaggtgaa actcaccggc gaactcgatc
    16681 tcgatctcgc ccgcgtgcgc gcgatccggg ccgcgcgccc cgacgtctgg ttgggagtcg
    16741 atgccaacca aggttattcg attggtgagc tgccggcgct ggtccgcggc ctttgtgagc
    16801 accgagtgct gctgctcgag caaccgctgc cgcgcggccg ggaggccgat ctgcagggct
    16861 tcgactcggt gatccccatc gcggcggacg agagcgttct ctcgctcgcg gagctgccgc
    16921 gtgcggtcgg gagtttcgac gtcgtcaaca tcaagctcga caaatgcggc ggcctcaccg
    16981 agggcctgat gatcgccgaa gaagcacggc ggctcgggct cgaagtgatg gtcggcacca
    17041 tgatcgggac gagtctcgcg accgcgcccg ctttcatcct tgcgcagcaa tgttcgatcg
    17101 tcgatctcga cggtccgact ttcctcgtca aggaccgctc gcctgcagtc gtctaccgcg
    17161 acggagatct gttcgctggc gacgacgtgt ggggcgccgc cagctccgtg gctgcatgat
    17221 ggccgtcgcc gcatgacgag cgagaagacc tggttcggcc atccccgtgg cctgaccgta
    17281 ctgttcttca cgaacatgtg ggagcagttc tcctactacg gcatgcgcgc gatcctggtc
    17341 tattacatga ccaagcagct gctgctggcg caggagactt cttcggtcat ctacggcatc
    17401 tacaccggct gcgcatattt cacgccgctg atcggcggat tcatcgccga tcgcttcctc
    17461 ggcaagaaac gcgcgatcat catcggcggc agcgtgatgg cgctcgggca tttcatgatg
    17521 actgccgagc cgctgttcta cgtggcgctg gcgactatcg cgatcggcaa tggtttgttc
    17581 ctgcccagcc tgccgagcca gattcgcgat ctgtaccagc ctggagatcc gcgcgccggc
    17641 cgcgcttaca acgtctacta cgtcggtgtg aacatcggcg gatttctcgc gatgctggtc
    17701 tgcggggcgc tgggcgaagc cttcgggtgg cactggggct tcggggctgc gggcgtcggc
    17761 atggtgaccg gtctcctttt ctacctgtgg ggtcaacgct atcttccggc gcagacccag
    17821 ttgcctcgcg ccgagctact cgcggcaccc cgtgaaaaca tggcgcgcga cacgctgctg
    17881 ctgctcttca gcctgggtgt ggcggtcgcc gtgttccgga gcgcgtacga gcaggtcggc
    17941 aacaccgtgg cgctgtggtt cgatacgggt gtctacaggc tggcggatgg attctcgatt
    18001 ccatttacat ggtttttcgc gatcaacccg ctctgcgtga tgttgatcac accgttcctg
    18061 ctcgcctggt ggcggaaacg tgaagcggca ggacacactc cattgcccac gcagcgcatg
    18121 gcgaccggcg cgctcatcgt tggcgcttcc tatctattcc tcgccgcgct ctcctggaca
    18181 ggggacagcg gcaccgttca ctggcaatgg ttcgcgctgt tctgggtggt gctgacattc
    18241 ggcgagctgt tcattcttcc cactggcatc gggctgtttg cccggctcgc gccgcaacga
    18301 ctgggcgcga caacagtggc ctcgtggttt ctggccacct tcgccggaaa cctggccgcc
    18361 ggcctggttg gaaccttctg gtcgcgcatg agccccccgg ccttcttcgt gttgctcgcc
    18421 gggatttgcg tactcgcggc cactgcgctt cggctgctcg atgcgcgcgc gcagcacgtc
    18481 accacgatca ctcgcccgca agccaaccct gattgacaat tctctaatca ggacattagt
    18541 gtccgtatca gagtaattgg ggcttaatag ggttgaaacc gatccgcgag aagtcggcct
    18601 caaccatggg gagaacgaac acatgactac ccgtacgaga attttccggt cgactggcgc
    18661 gttatcggcg ttgctgatga tgcaatccgc ctgggcgcag gatcggcccg acgtaacgaa
    18721 cctcgatgag gtcctcgtga ccgcccagaa ggtttcgcaa tcagtgatcg acgtaccgct
    18781 ggcgatttcg gtcctgggct ccgacgatct cgaacgcact caatccctga acttccagga
    18841 ctacacgaag ctcgtaccgg gtctgcagct cacgcaagcg tcgccgggct tcgggcgcct
    18901 ggtgatacgc ggcatcaaca cgggcggctc cgcgtcgacc gtcggcgtgt acgtcgatga
    18961 gacgcctttc ggttcgagca gcggcatcgc gaacggcgcc attctcgccg gcgatttcga
    19021 caccttcgac gtcgcgcgtg tcgaggtaat gcgcggtccg cagggcacgg tgtatggcgc
    19081 gagttcgttg ggtggcgtgc tcaagtacgt gacgaacgaa ccgcagttcg attcgttcga
    19141 agggcgagtg cgcggttcgt tggaaacggt cgatgatggc gactcgtcct ggtccggtgc
    19201 cgcggcggcc aatattcccg tgtccgacag cgtggcaatt cgcgcttccg gtttctatcg
    19261 caacgtgggc ggcttcattg attcgatcgg cacgggcgga tccgacgtag cagacgacat
    19321 caacggttcc accgttgcgg gcggacgcgc gtcgatgctc ttcaagccga gcgaggcctt
    19381 ctcgctgcag ctgaccgcct tcattcagga catcgcgaat gacgcgtcga gtgtcgtcga
    19441 ttcggatccg atgaccggcg agtcgctgta tggcggcctg acgcaatctc aattcgttcc
    19501 cgagtccgcg gatgtcagct atcgcgtcta caacgccacg gccatctggg atctcggctt
    19561 cggggatctg accagctcca cgagttatgg ctcgaacacg caggattttc gcgtcgattt
    19621 cacgacgctg ctcaggaatg ccttcggtct cgacatattc gattcctaca accagcagac
    19681 cacgcgcgtg aagcgcttca cgcaggaact gcgcctggcc tcgcacgaaa acgaaaaagt
    19741 cgactggctg atcggcgcct actacaacga tgaagacggt ttgatctacc agaacgtcca
    19801 tgtcgtcgag ccgggtacgc tgaacgacat
//
LOCUS       DASQJS010000269         3846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1729980, whole genome shotgun sequence.
ACCESSION   DASQJS010000269 DASQJS010000000
VERSION     DASQJS010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729980"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..492)
                     /locus_tag="VEW08_10840"
     CDS             complement(202..492)
                     /locus_tag="VEW08_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HYJ41282.1"
                     /translation="MTKLYVGNLPFSATEDSVRALFATHGNVDKVALVNDRDTGQPRG
                     FGFVEMSSADAQKAMQSLNGQDFGGRPLKVNEAQDKPRGGGGGGGGGGYGRR"
     gene            complement(655..1335)
                     /locus_tag="VEW08_10845"
     CDS             complement(655..1335)
                     /locus_tag="VEW08_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HYJ41283.1"
                     /translation="MRRALSSFTMAAILLAASNASQAQAPDPRQRFEKKVQEYEASDR
                     VSPPPRGAILFAGDSQFYRWKTIHEDLAGYTLINRGIDSFQFPDLLRYFDRLVLPYEP
                     RLIVLHVGGNDVHNGRTPAQVLADLQAFVSRVRAALPGVRVVYSSITPGPGRWDEAPQ
                     RVVTNQAVRDYIATQPDLAFVDLWEAMLTPTGQPREELWVEDRVHPNHAGYLIRVKQT
                     VPLLGAPR"
     gene            1375..1767
                     /locus_tag="VEW08_10850"
     CDS             1375..1767
                     /locus_tag="VEW08_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41284.1"
                     /translation="MIGELEEIFLRACPSFEVPLKQLREDHKLFGADLQTELTEILDK
                     LGFHLGSTAKQVPTADMIQVLKVVERVLMDGSEQQKWAVTHFLMTSLQTSIRDAGHRT
                     DALLPHLGPATASWWNDLTKNPRAGDDW"
     gene            complement(1771..2904)
                     /locus_tag="VEW08_10855"
     CDS             complement(1771..2904)
                     /locus_tag="VEW08_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HYJ41285.1"
                     /translation="MKVPPFKLDAWLMAHEFATPPIRFNIASSTGPLWTLAELMALGD
                     DKDGNRGGGGTEALGDVTLSYAPPQGSKLLRERVAALHGVEPDDVLMMTGASEAIMAL
                     TCLFAEPGASILIPAPVYPAVPVLARAWGLGVREYALDRAQGFAQTADGVLAAVDAST
                     RVVFVNTPHNPTGSVMPPAEQRKLAETLAARGIPLIVDEVYHPLYFAGPAPSASVLPN
                     TIVLGDFAKALSIPGLRIGWLIDRDARHREALLDMRSYFTISGSPLTEAIGAHALAHA
                     PEIIARLTQVVRGNLALFTRFMDEHCGVLDWAPPGGGTTCFPWLRDGRDARPMCEALA
                     KAGVLAAPGDCFEMPSHFRIGFGAMRSGYAEALGIFRAVLAKL"
     gene            3003..3491
                     /locus_tag="VEW08_10860"
     CDS             3003..3491
                     /locus_tag="VEW08_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41286.1"
                     /translation="MAANIPGAGEWAGYEDDLDARYAHGFWSGKSLDDMQPHFSNGRS
                     IERGSELLFMPRRAFQFYVFAFAQYVMSDTAIGDSDAASCFLNYLIGREKRDPGSVAQ
                     IYERLEPTLEFVAASQARFDASHDIYGDFAEKVEELKMLCGTTHSQRNPEDQMLDPTD
                     DA"
     gene            3491..>3846
                     /locus_tag="VEW08_10865"
     CDS             3491..>3846
                     /locus_tag="VEW08_10865"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HYJ41287.1"
                     /translation="MDQPDYQTMLEVAIAEARRGLEEGGIPIGAALFHSSGKLLGCGH
                     NRRVQEGDPSVHGETDAFRKAGRQRRYRDTIMVTTLAPCWYCSGLVVQFQIGTVVVGE
                     SRTFQGGIDWLRERGV"
BASE COUNT          733 a         1227 c         1239 g          647 t
ORIGIN      
        1 gaattcgaaa ttgaggcgga ccgacgcgaa ttcagggaac tgcacgcgca gctctttggc
       61 agccatgcga ttgcgccgct gcttctcgcg aagaagattg cgtgcccctt cggggccgac
      121 cgtggtgaat ttcatgtgtg cctcgatgcc gcgaccggcg cggatggcgt tgccgccatc
      181 cgcgtcgtca cgactgatgt cttaccgacg accgtagccg ccgccaccac caccgccgcc
      241 accgccgcgg ggtttgtcct gcgcttcgtt gaccttgagc gggcgaccgc caaagtcctg
      301 gccattcagc gactgcatcg ccttctgcgc atcggcgctc gacatttcca cgaagccgaa
      361 gccgcgcggc tggcccgtgt cacggtcgtt cacgagggcg actttgtcca cgttgccgtg
      421 ggtcgcgaac agggcacgca ccgaatcttc ggtcgcgctg aagggaagat ttccaacgta
      481 aagtttggtc aagagaatac tcactaagta gatgtagata ggcaggggaa ccctgcgaga
      541 gcgactctgg ccaaacgaca cgtcaggaag acgcgaaagc ccaggggcgg acaagacgag
      601 gaatcacaag ccgggcgcga atgtacagga aaaaccgtct ctttgccgca gttcttaacg
      661 aggcgccccc agcagcggca ctgtctgttt cacacgaata aggtaacccg cgtggttagg
      721 gtgaaccctg tcttccaccc acaattcctc acgcggctgc ccggttggcg tgagcatggc
      781 ctcccacagg tccacaaagg cgagatccgg ctgcgttgcg atgtagtccc gtacggcctg
      841 gttggtcacc acccgctgtg gggcctcatc ccagcgcccg gggcccgggg taatgctcga
      901 atacacgacg cgcactcccg gaagcgcggc gcgcacccgg ctgacgaaag cctgcaaatc
      961 cgcgagcacc tgcgcgggcg tgcgcccatt gtgaacgtcg tttccgccca cgtgcagcac
     1021 gatgaggcgc ggttcatagg gcagcaccag ccggtcgaaa taacgcagaa gatcgggaaa
     1081 ctggaaagaa tcgatgccgc ggttgatcag cgtgtagccg gccaggtcct cgtgaatggt
     1141 cttccagcga tagaactgcg aatcgcccgc gaacaggatg gcgccgcgcg gcggcgggct
     1201 gactctgtcg ctggcttcgt attcctgaac ctttttctca aaacgctgcc gcgggtccgg
     1261 cgcctgcgct tgcgaagcat tggacgctgc cagcaggata gctgccatgg tgaagctcga
     1321 taatgcgcgg cgcatgaaca ttccgcattc ccccctcgat gcctcggacg ttacatgatc
     1381 ggcgagctcg aagagatatt cctgcgcgcc tgcccttcgt tcgaagtccc tctcaagcag
     1441 ttgcgcgaag atcacaaact attcggcgcg gatctgcaga ccgagttgac cgaaatcctc
     1501 gacaagctcg gatttcacct gggcagtacg gcgaagcagg tgcccacggc agacatgatc
     1561 caggtgctca aggtggtcga gcgcgtgctc atggatggca gcgaacaaca gaagtgggcg
     1621 gtcacgcatt ttctgatgac gtcgctgcag acctcgatca gggatgccgg ccaccggacc
     1681 gacgcgctgc tgccacatct cgggcccgcc accgccagct ggtggaatga cctgacgaag
     1741 aatcctcgcg ccggcgacga ttggtaatag ttagagcttc gcgagcacgg cgcgaaagat
     1801 cccgagcgcc tcggcatacc cgctgcgcat ggccccgaag ccaatccgga aatgcgacgg
     1861 catctcgaag caatcgcccg gcgcggccag cacacccgct ttcgccagag cctcgcacat
     1921 cggccgcgcg tcgcgaccgt cgcgcagcca ggggaaacag gtggtgccgc cgcccggcgg
     1981 cgcccagtcc agaacgccgc agtgctcgtc catgaaccgc gtgaacaacg cgagattgcc
     2041 gcgcaccacc tgggtgagcc gcgcgatgat ttcaggcgcg tgggcgagcg catgcgcacc
     2101 gatggcctcg gtcagtggcg agccggaaat cgtgaagtag ctgcgcatgt cgagcagcgc
     2161 ctcacggtgg cgtgcgtcgc gatcgatgag ccagccgatg cgcaggcctg gaatcgacag
     2221 cgccttggcg aagtcaccga gcacgatggt gttgggcagc acgctggcgc tgggcgcggg
     2281 cccggcgaaa tacagcgggt gatacacctc gtcgacgatg aggggaatgc cgcgcgcggc
     2341 cagcgtctcg gcgagcttgc gctgctcggc cggaggcatc acagaacccg tcgggttgtg
     2401 aggcgtgttg acgaatacca ctcgcgtgct ggcatccacg gccgccagca cgccatccgc
     2461 ggtctgcgca aaaccctgcg cgcgatccag cgcgtactca cgcaccccga gcccccaggc
     2521 gcgcgccagc accggcaccg cggggtacac gggcgcggga atgaggatgg acgcgccggg
     2581 ctcggcgaac aggcacgtga gcgccatgat cgcttcggac gcgccggtca tcatgagcac
     2641 atcgtcaggc tcgaccccgt gcagtgcagc gacccgctcg cgcagtagtt tgctgccctg
     2701 gggtggtgcg taggacagcg tgacgtcgcc gagcgcttcg gtgcccccac ctccccggtt
     2761 gccatccttg tcatcgccaa gggccatgag ctccgcaagg gtccacagcg gaccggtgct
     2821 cgaggcgata ttgaagcgaa tgggcggcgt cgcgaattcg tgggccatga gccaggcgtc
     2881 gagcttgaat ggagggactt tcatggcgtt atgatcctgc gcatgccgcg cgatctcaat
     2941 gccgcctcga agggatgagc gtgcgggcgt atcttgactc cgctgaatcc gaggtaaccg
     3001 cagtggcagc gaatattccc ggtgccgggg aatgggccgg atacgaggac gacctcgatg
     3061 cgcggtatgc gcacggcttc tggtctggaa agtcgctgga tgacatgcag ccgcactttt
     3121 ccaatggccg gtcgatcgag cgcggcagcg aacttctctt catgcctcgc cgcgcgttcc
     3181 agttctacgt gttcgcgttc gcgcagtacg tgatgtcgga caccgccatc ggcgactcgg
     3241 acgcagccag ttgcttcctc aactatctca tcggccgcga gaagcgcgac cccggcagtg
     3301 tcgcccagat ctacgagcgg ctcgaaccga cgctcgagtt cgtggcggcg agccaggcgc
     3361 ggttcgatgc cagccacgac atctacggcg acttcgccga aaaagtcgaa gagctgaaga
     3421 tgctctgcgg cacgactcat tcgcaacgca acccggaaga tcaaatgctc gacccaacgg
     3481 atgacgcctg atggaccaac ctgactacca gaccatgctc gaagtcgcga tcgcggaggc
     3541 gcgtcgcggc ctcgaggaag gcggcatccc catcggcgcc gcgctgtttc actcgagcgg
     3601 caagttgtta ggctgcggcc acaaccggcg cgtgcaggaa ggcgaccctt cggtgcacgg
     3661 cgagaccgac gcgtttcgca aggccgggcg gcagcgccgc tatcgcgaca ccatcatggt
     3721 caccacgctc gcgccctgct ggtattgcag cggcctggtg gtgcagttcc agatcggtac
     3781 cgtcgtggtg ggtgagtcgc gcacctttca gggcggcatc gactggctgc gcgagcgcgg
     3841 cgtgaa
//
LOCUS       DASQJS010000270         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1416392, whole genome shotgun sequence.
ACCESSION   DASQJS010000270 DASQJS010000000
VERSION     DASQJS010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416392"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VEW08_10870"
     CDS             complement(<1..397)
                     /locus_tag="VEW08_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HYJ41288.1"
                     /translation="MSTSERPKGSSLKPLRALLPFLAPYRGRMLVAFLALLVAAAAML
                     ALPQALKNVIDKGFSAANAAAIDRYFLWLLVAAAVFAAFASLRMYLVNWIGERVVADL
                     RSAVYARVIRMDPAFFEVTKTGEVLSRLTT"
     gene            complement(384..725)
                     /locus_tag="VEW08_10875"
     CDS             complement(384..725)
                     /locus_tag="VEW08_10875"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HYJ41289.1"
                     /translation="MDIRAATPKDSGFRSFGEFYAYYLTEHRHPVSRVLHYIGTWGAV
                     ICVVALIVSREPWFLLGALLSGYTFAWFGHFRFEHNRPATFRFPFYSLASDFRMWWEL
                     NLGKLKFREHQ"
     gene            complement(738..>2525)
                     /locus_tag="VEW08_10880"
     CDS             complement(738..>2525)
                     /locus_tag="VEW08_10880"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HYJ41290.1"
                     /translation="VNPELSQRIELYATLIPGALGITPMRHDRMPYRVFLGQVSERLR
                     LTYDRRPNGYEGPQQFLRDLKLITASLKANKGFHAGWGNVQRLIRRVETFGFHLATLD
                     LRQHAEIHHRVIGQGLDDPQWMTRTPAERHDLLVQAIERDAGVKIELDALGRRTLGVF
                     EAILQARHRYGRDAIGYYVVSGTQNADDVLAPLLLARWAEAYDRVSGEVAVDIAPLFE
                     SVDSLEHCGDVMKTLLGDPLYRRHLDARARTQCVLIGYSDANKESGICSSRFAAYRAQ
                     ADLSTALAAANERHVIFHARGGSIARGGSRIDSLVRTAPAGTVNGVLRLTEQGEVINQ
                     SYGLKPIAMRTMERAFNALALSLGGAVAGSAPKPEQLQFAARLAVVSRQVYRGFVHGD
                     ADFYAWFQAVTPIDVIARMQIGSRPAERPGKEGLDALRAVPWVFAWTQSRHMLPAWFG
                     AGSGLRTAVEEVGLGAAQSAYNEWNFFTTLIDDLETSLSRADLDIAAAYEDLAESRQR
                     HFGVKLRQEFGLVVEQVLAIKQTRMLLDRDQTMQRSIELRNPYVDPVNLLQVDLLRRW
                     RDTGRKDRDLFESLLACIAGIAEGLQSTG"
BASE COUNT          460 a          821 c          832 g          412 t
ORIGIN      
        1 cggtagttag cctggagagc acctcgccgg tcttggtcac ctcgaaaaac gccgggtcca
       61 tgcgaatgac ccgcgcgtac accgcgctgc gcaggtccgc caccacgcgc tcgcctatcc
      121 agttcacgag gtacatgcgc agcgaggcga acgcggcgaa caccgcggcg gccaccagca
      181 gccacaggaa atagcggtcg atggcggcgg cgttcgcggc gctgaagccc ttgtcgatga
      241 cgttcttgag cgcctgcggc agcgccagca tggcggcggc ggccacgagc agcgcgagaa
      301 acgccaccag catgcgcccg cgatatggcg cgaggaacgg cagcaacgcc cggagcggtt
      361 ttagggagct gcctttcggg cgttcactgg tgctcacgga acttgagctt tcccaagttc
      421 agttcccacc acatgcggaa atcgctcgcc aggctgtaga acggaaagcg aaacgtcgcc
      481 ggccggttgt gttcgaaccg gaaatggccg aaccacgcga aggtgtaacc gctgagcagc
      541 gcgcctagca ggaaccacgg ctctctggag acgatgagcg ccacgacgca gatgaccgca
      601 ccccaggtgc cgatgtagtg cagcacacgc gacaccgggt gacggtgctc cgtcaggtag
      661 taggcgtaga actcgccgaa gctgcgaaaa cccgagtcct tgggtgtggc ggcgcgtatg
      721 tccataggtg gccgacatca tccagtactc tgtagccctt cggcaatccc cgcgatgcag
      781 gcgagcagcg attcgaacaa atcacggtcc ttccggccgg tatccctcca tcgccttagt
      841 agatcgacct gaagcagatt gacgggatcc acgtatggat ttcgcagctc gatggatctc
      901 tgcatggtct ggtcgcggtc gagcagcatc ctggtctgct tgatggccag cacctgctcc
      961 accaccaggc cgaactcctg gcgtagtttg acgccgaaat gccgctggcg gctttccgcg
     1021 aggtcttcgt aggccgcggc gatgtcgaga tcggcgcgcg acagcgaagt ctcgaggtca
     1081 tcgatcaggg tggtgaagaa attccactcg ttgtacgcgg actgcgccgc gccgagcccc
     1141 acttcttcga cggccgtgcg caacccggat ccggcaccga accaggccgg cagcatgtgg
     1201 cgcgactgcg tccaggcgaa cacccagggc actgctcgca gcgcatcgag gccttccttg
     1261 cccggacgct cggcgggccg tgacccgatc tgcatgcgcg cgatcacgtc tatgggcgtc
     1321 accgcctgga accaggcata gaagtccgcg tcgccatgca cgaagccacg gtagacctgc
     1381 cggctgacca cggccagccg cgccgcgaac tgcaactgtt cgggcttcgg cgcactgccg
     1441 gcaaccgcgc caccgaggga cagcgcgagc gcgttgaagg cgcgctccat cgtgcgcatg
     1501 gcgatgggct tcagcccata actctggttg atgacctcgc cctgctcggt caggcgcagc
     1561 accccattca ccgttccggc cggagccgtg cgcaccaggg aatcgatgcg tgaaccaccg
     1621 cgcgcgatgc tgccgccgcg cgcatggaaa atgacatggc gctcattggc cgcggcaagc
     1681 gcggtggaga gatccgcctg cgcgcggtac gcggcgaagc gcgaactgca gatgccgctc
     1741 tccttgttgg cgtccgaata cccgatgagg acgcactgcg tgcgggcacg ggcatcgaga
     1801 tgccggcggt agagcgggtc gcctaacaag gttttcatga cgtcgccgca gtgttccagc
     1861 gaatcgacgg actcgaacag cggcgcgatg tcgacggcga cctcgccgga gacgcggtcg
     1921 taggcttccg cccaacgcgc cagcaacagt ggggccagca cgtcgtcggc gttttgcgtg
     1981 ccgctgacca cgtagtagcc gatggcgtcc cgcccatagc gatgccgcgc ctgcaggatg
     2041 gcttcgaaca caccgagcgt gcggcgaccg agcgcgtcga gttcgatctt gacgccggca
     2101 tcgcgctcga tggcctgcac cagcaggtcg tgccgctcgg cgggcgtgcg cgtcatccac
     2161 tgcgggtcat cgaggccctg gccgatgacg cgatggtgga tctcggcgtg ctggcgcaga
     2221 tcgagcgtgg ccaggtgaaa cccgaaggtt tccactcggc ggatgaggcg ctgcacgttg
     2281 ccccagccgg cgtgaaatcc cttgttcgcc ttcaggctgg ccgtgatcaa cttgagatcg
     2341 cgcaggaact gctgcggacc ttcgtaacca tttggccggc ggtcgtaggt gaggcgcagc
     2401 cgttccgaca cctgccccag gaacacgcgg tacggcatgc ggtcgtggcg catcggcgtg
     2461 atgccgagcg cgccgggaat cagagtggcg tacaactcga tgcgctggct gagctccgga
     2521 ttcac
//
LOCUS       DASQJS010000271         6026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3616984, whole genome shotgun sequence.
ACCESSION   DASQJS010000271 DASQJS010000000
VERSION     DASQJS010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3616984"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VEW08_10885"
     CDS             <1..604
                     /locus_tag="VEW08_10885"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41291.1"
                     /translation="SYWLLRERDKNLGIEDAVRELRAGVEQLIDSIDSAIGGDARTQH
                     DATFAGFVAGGVPDKLARRVARLTLLEPALDIVALARAEHMPVADVARVYFELGVLLG
                     LDWLHGEIDRLAVDGSWQATARTNLRDAAMRAHRELTHQVLNTRGAKRVGERLARWSA
                     QRADALTSWKRTLQEMRAVGTTDFATLTVGVESVRGMSSG"
     gene            608..1429
                     /locus_tag="VEW08_10890"
     CDS             608..1429
                     /locus_tag="VEW08_10890"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYJ41292.1"
                     /translation="MRLALVITTHERPEALAAVFDSVGRQRVPPDEIIIADDGSGAAT
                     QQSIAAFVSSSTVPVRVVTQPHEGFRAARLRNLGSAATSSDYLVFIDGDMLLHPLFIA
                     DHQHLARPRFYTQGVRVHTDASLTNELIAKPAALPSFWSAGLGGLRRLYLVRSPALAT
                     MARTIANGVVAIKSCNLGIWREDFVRVNGFNEAFEGWGPEDKELCVRLENAGVQRQTL
                     MFGGIALHLHHAPASRAALPANLARLAETRRARLVRCNRGVDAHIRSRAQGAQNE"
     gene            1469..2236
                     /gene="gpmA"
                     /locus_tag="VEW08_10895"
     CDS             1469..2236
                     /gene="gpmA"
                     /locus_tag="VEW08_10895"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HYJ41293.1"
                     /translation="MPGKSIPGKLILVRHGQSTWNVENLFTGWHDVDLSDLGRIEAKS
                     AGAEIRKAGIRPDIVFTSVLKRAIRTMWSVLDEMDLMWLPVERSWRLNERHYGALQGL
                     DKAQTVEKHGEAQVKIWRRSYDIPPPPLALDDKRHPIFDRRYANVDPRVLPASESLKD
                     TLARVLPFWNDRIVVELLAGKNVLVVAHGNSLRAVVKMLDNVSEAEITELNIPTGVPL
                     VYELDAGLKPIRSGYLGDPEAIKAAAEAVKKQTEKKK"
     gene            complement(2265..2708)
                     /locus_tag="VEW08_10900"
     CDS             complement(2265..2708)
                     /locus_tag="VEW08_10900"
                     /inference="COORDINATES: protein motif:HMM:NF018574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HYJ41294.1"
                     /translation="MSNSAIWMYVSAVVGGLIAGWRIWQNLNKLRTRKNDSWDERLID
                     QLRKSGSDPFKPHDVDFFLAFPALESAEALAARFRTEGFDTDVLDEPENGDLRYALHL
                     HKSMLLVVPDMKELSRRLTDAAKEKEGKYDGWSAKPVARTDLPSQ"
     gene            complement(2714..3028)
                     /locus_tag="VEW08_10905"
     CDS             complement(2714..3028)
                     /locus_tag="VEW08_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYJ41295.1"
                     /translation="MATLQVIDRDGVSHDVECKPGLKVMEVLRELDYGVAAICGGMCS
                     CATCHVYVDAAWVGKLPAPMSDERDLLTELSHYRENSRLSCQVEITAALSGLKVTIAE
                     DE"
     gene            complement(3006..3953)
                     /locus_tag="VEW08_10910"
     CDS             complement(3006..3953)
                     /locus_tag="VEW08_10910"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HYJ41296.1"
                     /translation="MIAGELLQHVPGCEDGEPPFSQEMIGGGKVNHSYLVRTRRGRFV
                     VRLNEYTAADPCLDRDRELALHTAAADAGIAPQVVYAAPDRSCLITDYVDGRLWTPHY
                     FTRMRDLRSLGQRLRTLHSLAPPPVTRFDPLATARIYADLIVRGDPNEGGRLQFLLAS
                     GAEALARSGFERRAPAIVHSDLHHGNVLTADRVYFIDWEYAQVGDPLFDLACIMGYYP
                     RAMPHGELLLEAAGLTDVGTTPAMLGELTNVFTLLTYLWYRARRVGRNVPATDLQIES
                     AALRRLLSLAPDAQPRHTSAANLRESSGFGKRNGDAAGD"
     gene            complement(3950..4522)
                     /gene="pnuC"
                     /locus_tag="VEW08_10915"
     CDS             complement(3950..4522)
                     /gene="pnuC"
                     /locus_tag="VEW08_10915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HYJ41297.1"
                     /translation="MLKTFEILATLAGVAYVVLAALRNRLCWIVGAFSSALIAVVAGW
                     SKLPMQSALQVFFVGMSVYGWWSWTRSSTEGELPVGVWPHRWHLAAALVAILLSILSA
                     QWLAAETRAAWPLLDSLTTWFSLLAVWLQARAKIENWLYWIAIDGVLVFLFYSTNRPW
                     LALLNALFIVIATAGFVGWRRRLHIQAVPA"
     gene            complement(4538..4780)
                     /locus_tag="VEW08_10920"
     CDS             complement(4538..4780)
                     /locus_tag="VEW08_10920"
                     /inference="COORDINATES: protein motif:HMM:NF019246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HYJ41298.1"
                     /translation="MNIGVEISLYPLHDDYIPPIRGFIERLNNDGRFKVMTNDMSTQV
                     FGQYEEVMEALTRELKPTFERDGKAIFVMKVLGPLG"
     gene            complement(4791..>6026)
                     /locus_tag="VEW08_10925"
     CDS             complement(4791..>6026)
                     /locus_tag="VEW08_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ41299.1"
                     /translation="ESFDVVSRSAFGTSRSVYSFDGDWGNDISWGANSPYDYFQRFDR
                     EHTTLSTDLRIVSRASVDNGDEFAWLAGVYALRTDEDVRQHDVYRDLIYGDGDRVFTS
                     NYRATNLAGYGELEWRLGTSTVLAFGARAEQRRADYRDSDEAAFSPNETMFGGSLSLR
                     HALGDQSTAYVTLARGYKAGGFNIGDAVPADRRSFDSEVLHNLEVGFRATSADATLAG
                     DIALFYMRREDQQVQTGEQLVPGDPLTFVQYTDNAAAGENYGIEATLQWRPVSPLLLD
                     LRAALLETRYIDYVFGDRNLDGREQAHAPQYQYDLGVEYRGARGAFVRVDFAGLDDFY
                     FDDSHDERAPSRMLTHLKAGLSGKHWRAEVWVRNLFDRYYSQRGFYFGLEPPDFTPTR
                     YVQAGDPRHAGVTVTYTFR"
BASE COUNT         1156 a         1948 c         1894 g         1028 t
ORIGIN      
        1 cagctactgg ctgctgcgtg agcgcgacaa gaatctcggc atcgaagatg cggtgcgcga
       61 gctgcgtgcc ggcgtcgagc agctgatcga cagcatcgac tccgcgatcg gcggcgatgc
      121 ccgcacgcag cacgacgcga cgttcgccgg cttcgtggcc ggtggcgtgc cggacaaact
      181 ggcgcgccgc gtcgcacgac tgacgttact cgaacccgcg ctcgacatcg tggcgctggc
      241 ccgtgccgaa cacatgccgg tcgccgacgt cgcccgcgtg tatttcgagc tgggcgtgct
      301 gctcggcctg gattggctgc acggtgaaat cgaccgtctc gcggtcgacg gctcctggca
      361 ggccacggcg cgcaccaacc tgcgcgatgc ggccatgcgc gcgcatcgcg aactcaccca
      421 ccaggtgctc aatacgcgcg gagcgaagcg tgtgggtgag cgcctcgcgc gctggagcgc
      481 gcagcgcgcg gacgcgctga ccagctggaa gcgcacgctc caggagatgc gcgcggtggg
      541 caccactgat ttcgccacac tcaccgtggg tgtcgagtcg gtgcgcggta tgtcgagcgg
      601 ttgaggaatg cgcctcgcgc tggtgatcac cacgcatgag cgccccgagg ccctggccgc
      661 cgtgttcgac agcgtcgggc gccagcgcgt tccgcccgac gaaatcatca tcgccgacga
      721 cgggtcgggc gccgccacgc agcaatccat agcggccttc gtatcgagtt ccaccgtgcc
      781 ggtgcgcgtg gtcacacaac cgcacgaagg gttccgcgcc gcgcgcctgc gaaacctggg
      841 cagcgccgct acatcatcgg actacctggt gttcatcgat ggcgacatgc tgctgcatcc
      901 attgttcatc gccgatcatc aacacctggc gcgcccgcgg ttctacaccc agggcgtgcg
      961 cgtgcacacc gatgcctccc tgacaaacga gctgatcgca aagcctgcgg cgctgccctc
     1021 attctggtcg gccgggctcg gcggcctgcg ccggctctat ctagtccgtt cgccggcgct
     1081 cgccacgatg gcgcggacga tcgccaacgg cgtcgtcgcc atcaagtcat gcaacctggg
     1141 aatctggcgc gaggacttcg tgcgtgtgaa tggtttcaat gaagcgttcg agggttgggg
     1201 tcccgaggac aaggaactat gcgtccggct cgaaaatgcc ggtgtccaac gccagacgct
     1261 gatgttcggt ggcatcgcct tgcacctgca ccatgcaccg gcatcgcgcg ccgcgctgcc
     1321 cgcgaatctg gcgaggctcg ccgagacgcg ccgagcgcgg ctggtccgat gcaaccgcgg
     1381 cgtcgacgcg catatccgca gtcgcgccca gggcgcgcaa aacgaatgat ctgcggctaa
     1441 gattgcgcac cccaatgtag gagcacgcat gcccggcaaa tccattcctg gaaagttgat
     1501 actcgttcga catggccaga gcacctggaa cgtcgagaac ctgttcaccg gctggcatga
     1561 cgtcgacctg tcggatctgg gacgcatcga ggcgaagtcg gcgggcgccg aaatcaggaa
     1621 ggccggcatc aggcccgaca tcgtgttcac ctccgtgctc aagcgcgcca tccgtacgat
     1681 gtggagcgtg ctcgacgaaa tggacctcat gtggctgccg gtggaacgca gctggcgcct
     1741 caacgagcgc cactacggcg cgctgcaggg tctcgacaag gcccagaccg tggagaagca
     1801 cggcgaggca caggtgaaaa tctggcgccg cagctatgac attccgccgc cgccattggc
     1861 gctcgacgac aaacgccacc ccattttcga tcgccgttat gccaacgtgg atccacgcgt
     1921 gctgccggct tcggaatcgc tcaaggacac gctcgcgcgc gtgctgccgt tctggaatga
     1981 ccgcatcgtg gtggaactgc tggcgggcaa gaacgtgctc gtggtggcgc acggcaactc
     2041 cctgcgcgcc gtggtgaaga tgctcgacaa cgtgagcgaa gcggagatca ccgagctcaa
     2101 catccccacc ggcgtgccgc tggtgtacga gctcgacgcc ggtctcaagc ccatcaggag
     2161 cggctatctc ggcgatccgg aggccatcaa ggctgccgcc gaggccgtga agaagcagac
     2221 cgagaaaaag aagtagctag atccaaaatc cgcgcggaac ggctttactg cgaaggcaga
     2281 tccgtccgcg ccactggctt cgcgctccag ccatcgtatt tcccttcctt ctctttcgcc
     2341 gcgtccgtga gccgccggct gagttccttc atgtccggca ccaccagcag catcgacttg
     2401 tgcaggtgca gggcgtaacg cagatcaccg ttctcgggtt catccagaac gtccgtgtcg
     2461 aatccttcgg tgcgaaatcg cgcggctaac gcttcggccg actcgagcgc cggaaacgcc
     2521 aggaagaaat ccacgtcgtg cggcttgaaa gggtccgacc cggacttgcg caattggtcg
     2581 atcaaccgct catcccacga atcattcttg cgcgtgcgca gcttgttgag gttctgccag
     2641 atgcgccagc ccgcgatgag gccgccgacg acggcgctga cgtacatcca gatggcggaa
     2701 ttcgacacga ggattactcg tcttcggcga tggtgacttt gaggcccgac aacgcggctg
     2761 taatttccac ctggcaggac aggcgcgagt tctcgcggta gtgcgacagc tcggtcagca
     2821 aatcgcgctc atccgacatg ggagcgggaa gtttaccgac ccacgccgca tcgacgtaga
     2881 cgtggcaggt ggcgcaggaa cacatgcccc cgcagatggc cgcgacgccg tagtccagct
     2941 cgcgcaacac ctccatcact ttcagacccg gcttgcactc cacatcgtgg gaaacaccgt
     3001 cccgatcaat cacctgcagc gtcgccattc cttttcccga aacctgaaga ttcgcgcaaa
     3061 ttcgcggcgc tagtgtgccg aggttgcgcg tcgggcgcca gtgaaagcag gcgccgcagg
     3121 gccgcggatt cgatctgcag gtcggtggcg gggacgttgc ggccaacccg gcgcgcgcgg
     3181 taccagagat atgtcagcag cgtgaatacg ttggtgagct cacccagcat cgcgggcgtg
     3241 gtgcccacgt cagtgagccc agccgcttcc agcaacagtt cgccatgcgg catcgcgcgc
     3301 gggtagtagc ccatgatgca ggcgagatcg aacagcggat cacccacctg cgcgtattcc
     3361 cagtcgatga aataaacgcg gtccgcggtc agtacattgc cgtgatgcag gtcgctgtgc
     3421 acgatggccg gcgcgcgcct ttcgaagcca ctacgcgcca gcgcttcggc gccgctggct
     3481 aacaagaact ggagtcgccc gccttcgttg gggtccccgc ggacgatcag gtcggcataa
     3541 atgcgcgccg tggccaacgg atcgaagcgc gtcaccggcg gcggagccag cgagtgcaac
     3601 gtgcgcaggc gctggccgag tgaacgaagg tcccgcatgc gcgtgaagta gtgcggcgtc
     3661 cacagtcgtc cgtcgacgta atcggtgatc aggcaggatc ggtcgggcgc tgcgtagacc
     3721 acttgcgggg cgatgcccgc atccgccgcc gcggtgtgca gcgcaagctc gcgatcgcgg
     3781 tcgaggcagg gatccgcagc cgtgtactcg ttgaggcgaa cgacgaaacg gccgcggcgc
     3841 gtgcgcacca ggtagctgtg attgaccttg ccgccgccga tcatctcctg tgagaacggt
     3901 ggttcaccgt cttcacagcc cggcacgtgc tgcagcaact ccccggcgat catgccggca
     3961 ccgcctggat atgcaaccgg cgacgccagc cgacgaagcc ggcggtcgca atgacgatga
     4021 acaatgcgtt gagaagcgcc agccagggcc ggttggttga ataaaagagg aacacgagca
     4081 ctccgtcgat cgctatccaa tagagccaat tctcgatctt tgcgcgcgcc tgcagccaga
     4141 ccgcgagcag actgaaccac gtcgtcaatg aatcgagcaa cggccaggcc gcacgcgttt
     4201 cggcggcaag ccattgcgcg ctcagtatcg acaacagtat cgcaacgagc gctgccgcga
     4261 ggtgccagcg atgcggccac acgccgacgg gtagttcgcc ttccgtgctg ctgcgcgtcc
     4321 agctccacca gccgtacacc gacataccca cgaagaacac ctgcagcgca gactgcatcg
     4381 gcagcttgct ccagccggcg accacggcga tgagcgccga gctgaacgca ccgacgatcc
     4441 agcacagccg attgcgaagc gcagccagca ctacataggc aacgccggcg agcgtggcaa
     4501 ggatctcaaa ggttttgagc acttcgggcg gcaagatcta accgagcggg cccaggacct
     4561 tcatgacgaa aatggccttg ccgtcacgtt cgaacgtggg cttgagctct cgtgtgagcg
     4621 cttccatgac ttcttcgtac tggccgaaga cctgcgtact catgtcattg gtcatgacct
     4681 tgaaacggcc atcattgttc aggcgctcga tgaatccccg gatcggcgga atgtagtcat
     4741 cgtgaagggg atacaggctg atctcgacgc cgatgttcat ctggtctccg tcaacgaaat
     4801 gtgtacgtca ccgtcacgcc cgcatgacgc ggatcgcccg cctgcacgta acgcgtcggc
     4861 gtgaaatccg gcggctcgag gccgaaatag aatccacgct gcgaatagta cctgtcgaac
     4921 aggtttctga cccaaacttc cgcgcgccag tgtttccctg agaggcccgc cttcaggtgc
     4981 gtcagcatgc gcgacggtgc acgttcatcg tgcgaatcgt cgaagtagaa atcgtcgagc
     5041 cccgcgaagt cgacgcgcac gaatgcgccg cgtgcaccgc gatactccac gcccaggtcg
     5101 tattgatact gcggtgcgtg cgcctgctcg cggccgtcga gattgcgatc accgaagacg
     5161 tagtcgatgt agcgtgtctc gagtagtgcg gcgcgcaggt ccagcaacaa gggggagacc
     5221 ggccgccact gcagggtcgc ctcaatgccg taattctcgc ccgctgcggc gttatcggtg
     5281 tattggacga aggtcagcgg gtctccgggc accaactgct ccccggtctg cacctgctgg
     5341 tcctcgcggc gcatatagaa gagcgcaata tcgccggcga gcgtggcgtc ggcgctggtc
     5401 gcgcggaatc cgacttcgag attgtgcagc acttccgaat cgaagctgcg gcggtcggcc
     5461 ggaaccgcgt cgccgatatt gaatccccca gccttgtagc cccgcgccag cgtgacatac
     5521 gcggtgctct gatcgcccag ggcatggcgc aggctgagag atcccccgaa catggtctcg
     5581 ttgggagaga aagccgcctc atccgaatcc cggtagtcag cccgccgctg ttcggcgcgc
     5641 gcaccgaaag ccagcaccgt cgatgtgccc agacgccatt cgagctcacc gtagccggcg
     5701 agattcgtgg cgcgatagtt gctcgtgaat acccgatcgc cgtcaccgta aatcaagtcg
     5761 cgataaacat cgtgctgccg cacgtcttcg tccgtgcgca aggcgtagac acccgcgagc
     5821 caggcaaact catcgccgtt atcaacggaa gcgcgcgaca caattcgaag gtcggtgctc
     5881 agggtcgtgt gttcgcggtc gaaccgctgg aaatagtcgt acggcgagtt cgcgccccag
     5941 ctgatgtcgt ttccccagtc gccgtcgaat gaatagacac tgcgcgaggt tccgaacgct
     6001 gaacggctca cgacgtcgaa agattc
//
LOCUS       DASQJS010000272         6625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3311383, whole genome shotgun sequence.
ACCESSION   DASQJS010000272 DASQJS010000000
VERSION     DASQJS010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3311383"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3713
                     /locus_tag="VEW08_10930"
     CDS             <1..3713
                     /locus_tag="VEW08_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYJ41300.1"
                     /translation="FAWTSWRLFSGMFGQGGHFDLWQFIAAICATFLAVFMLKALFFI
                     QHRFDVEDIEITRQEQPRLFEFIDRLADEARAPRAHKVYLSARVNAAVFYDLSLLNLF
                     IPSKKNLEIGLGLVNVVTLGELKAVLAHEFGHFAQRSMAVGRWVYISQQIAGHIVARR
                     DALDRLLQQLSRFDLRVAWVGWILSVIVWSIRSMMDVLFRGVLLAQRALSRQMEFQAD
                     LVAVSLTGSDALIHALHKLSAADDAWDKALEFANTEAGAKRGITDLFAVQTRIIERMR
                     EILNQPLYGQVPPLPETARDSHRVFKTELAQPPRMWGTHPPSWDREHNAKQRYVSAPV
                     DERSAWEVFLHPQALKEQMSAHVYRNATVDPTPMEKTFQNLEKQYGRAFLNRAYRGVY
                     LHRSPVRYAGTVDALYGAAIAPADLVRVLDSLYPEGLALKVERLNEKLEEKHALIALR
                     DQVAQAPGGIIRHNGTEIRRTELPRAIANLEREITAVRGEIEAHDKLCRSAHHAAAKT
                     LEKGWPDYLRSLAAMLHYADHTEANLRDVQGYVANIYNVVTADGRVSSKELTRLLEGC
                     QQLYAVLNTVHGQANEVSLDRTLLRRLEVESWGALLEEFKLPPPNRENIGEWINVIDG
                     WIGAAIAAMARLRVAALEQLLLVEGQVAKFARENLKPADAPPASAVPKQFATLLPGKE
                     RPRQKRLDWWDRFQTADGVVATIARSAVALGIVGSVIAIGAHVGASSMTIYNALAVPV
                     RIEYGGEQIDVRPNHPTTVTVPAGGHLDVRAVTQSSELIEEFEADLHGANARYIYNVA
                     GAAPLYEWTAVYTPEGAGNTNPASAPSERQLGTQRWSTTAVDHVFEVPPEQIQTSSSS
                     GATYRNVLSAASDASPWRQVEMTTKPEEKSQLVMTHARWDNTSSLRMLEWLDLASKEP
                     EFGALFAARLKHEPKDVLLLRYEQDSSEGEKHDAVCARHRKLAETQPAEANLQYLRIR
                     CVSDEQEKNSQFASAREKWPDNPWLSMAAGITLAERGHYLQAQPMYDLARKRLPAMRG
                     YLALDHARLRRLNSVDGKVELADLVPNSDRLGMFVAIETGHDVDDTPLEAYAWLARGT
                     LDVAVLNANKNKEGRERVMRLVASSDGATRAMIDDALALPLDEKDDFETAIAMYAVAV
                     KALRDPSRHVAHLEKMLGPEGKPLLEFLEFLRRGGDPVQARTMLPEFDLQLRMHALHA
                     AVIMLDHRAPADWRKEASRGLFVGERGYLRNH"
     gene            complement(3806..4747)
                     /locus_tag="VEW08_10935"
     CDS             complement(3806..4747)
                     /locus_tag="VEW08_10935"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYJ41301.1"
                     /translation="MSKPLWHGVYPAATTQFAADLSLDIVACRQTVTALVDDGVDGLV
                     LLGTVGENNSLRPEEKRFALRAAVEAVGKRVPLVAGVSELSTDRAIEFVHDAEKIGID
                     ALMVLPAMVYVPTPEELCAHFRAIAEATSLPIMLYNNPPAYRVSIELETLERLAPLSN
                     IVALKESAPDPRRFTDIINRFGDRFTLMAGLDDVALEALLLGATGWVSGLTSAFPRES
                     VAMVAAADRGDWEEARRIYRWFMPLLHLDADHDLVQSIKLAEQIMGRGSERVRMPRMP
                     LSGERRKDVIARVERCAATRVKPRSGTHADLPARRQG"
     gene            complement(4851..>6625)
                     /locus_tag="VEW08_10940"
     CDS             complement(4851..>6625)
                     /locus_tag="VEW08_10940"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HYJ41302.1"
                     /translation="RCGVPMTNRVRMIAFMLLSVACLSMTHPSAAQSASSADERLRAL
                     YTAEWDWRQQEFARHPGEVGRGFTADHLPRVDAATQKARLAYWTSVLTQLDAIEPATL
                     SASERLNGEIFRAVVEELAVDARFKSYEAPFNADTFFWTSFTPREGFVTADEYRRYLG
                     RLRDAPRYFDEQIVNMRAGLKRGYTVPKVSVQGRDRTIEPYLAADDTNPLYLPFVRMP
                     ASMPADEQDRLRTEARGTIARSTVPAYTKLLAFIRTEYLVRARETLAASKLPDGAAYY
                     QAMIRKFTTLDLTAEQIHKIGLEEVARIRALQMDTMAKAGFKGTLPEFMVFLRTDPQF
                     YARTPRELLANAAFIVKKADLKLKETIGTLPRFRHGILPVPDALAPIYTGGRGGLESC
                     MFNTYNLSARPLYTLPSLVLHECTPGHSFQAALALEAPARPTFRNETYFSGYGEGWGL
                     YMEWLGNVMGIYETPYEEFGQLTYEMWRAVRLVVDTGIHHYGWTRARALDYMRSNIAL
                     SEHELTTEVDRYIAWPGQAVAYKLGELQIRRHRREAETALGPKFDQRKFHDAILAIGA
                     VPLPALERRMAQFIADGGENPSVD"
BASE COUNT         1247 a         2138 c         2088 g         1152 t
ORIGIN      
        1 ggtttgcctg gacttcgtgg cgcctgttca gcggcatgtt cggccagggt ggccacttcg
       61 acctctggca gttcatcgcc gcgatctgcg ccacgttcct cgcggtgttc atgctcaagg
      121 cgctgttctt catccagcac cgcttcgacg tcgaagacat cgagatcaca cgccaggagc
      181 agccgcgact gttcgaattc atcgaccggc tcgccgacga agcccgtgcg ccgcgtgcgc
      241 acaaggtcta cctctcggcc cgggtcaatg ccgctgtgtt ctacgacctt tcactgctga
      301 atctcttcat tccttcgaag aagaacctcg agatcggcct ggggctcgtc aacgtggtca
      361 cgctcggcga actcaaggcc gtgctggcac acgaattcgg acatttcgcg caacgctcca
      421 tggccgtcgg ccgctgggtt tacatctcgc agcagatcgc cggccacatc gtggcgcgcc
      481 gcgatgcgct cgacagactg ctgcagcagc tgtcgcgctt cgacctgcgc gtcgcctggg
      541 tggggtggat actctccgtc atcgtctggt cgatccgctc gatgatggac gtcctgttcc
      601 gcggcgtgct gctggcgcag cgcgcgctgt cgcggcagat ggaattccag gcagacctgg
      661 tcgccgtctc actgaccggc agcgatgcgc tgatccacgc gctgcacaag ctcagcgcgg
      721 ccgatgacgc ctgggacaag gcgttggaat tcgccaatac cgaggccggc gccaagcgcg
      781 gtatcaccga cctgtttgcc gtgcagacga gaatcatcga gcgcatgcgc gagatcctca
      841 accagccgct gtacggccag gtgccgccgc ttcccgaaac cgcgcgcgat tctcaccgcg
      901 tgttcaagac ggaactcgcg cagccgccgc gcatgtgggg cacgcacccg ccgagctggg
      961 atcgcgaaca caacgccaaa caacgttacg tttccgcgcc ggtggacgaa cgcagcgcct
     1021 gggaagtgtt cctgcatccc caggcactca aggaacagat gtcggcgcac gtctaccgca
     1081 atgcgacggt cgacccgacg ccgatggaga agacgtttca gaatctcgaa aaacaatacg
     1141 gccgcgcctt cctgaatcgc gcgtatcgcg gcgtgtacct gcatcgatcg ccagtccgat
     1201 acgccgggac cgtggacgcc ttgtatggag cggcgattgc gcccgccgat ctggtgcgcg
     1261 ttctcgattc gctgtatccc gaaggtctcg cgctgaaggt cgagcgcctg aatgagaagc
     1321 tcgaggaaaa acacgcgttg atagcgctgc gcgaccaggt tgcgcaggca cccggtggaa
     1381 tcatccgcca caacggcacc gagattcgcc gtaccgaact gccgcgcgcc atcgccaatc
     1441 tcgaacgcga gatcaccgcg gtgcgcggcg agatcgaggc gcatgacaag ctctgtcgca
     1501 gcgcgcacca cgcggccgcg aaaactctcg agaaaggctg gccagactac ctgcgttcgc
     1561 tcgccgccat gctgcactac gcggaccaca ccgaagcgaa cctgcgtgac gtgcagggat
     1621 acgtggccaa catctacaac gtggtcaccg ccgacggtcg cgtgagctcc aaggaactca
     1681 cccggctgct cgaaggctgc cagcagctct acgccgtgct caataccgtt cacggccagg
     1741 cgaacgaggt ttcgctcgat cgcacactgt tgcggcgcct ggaggtggaa agctggggag
     1801 ccctgctcga ggagttcaaa ctaccgccgc cgaatcgcga aaacatcggc gagtggatca
     1861 acgtgatcga tggctggatc ggcgcagcga tcgcagccat ggccaggttg cgcgttgcgg
     1921 ctctcgagca gctgctgctg gtcgaaggcc aggtggcgaa attcgcgcgc gaaaacctga
     1981 aacctgcgga cgcgccgccc gcctccgccg tcccgaagca attcgccacg ctgctgcccg
     2041 gcaaggagcg tccgcgccag aaacgtctcg attggtggga ccgcttccag accgccgacg
     2101 gcgtcgtcgc gaccatcgcg cgctcggcgg tcgcgctggg aatcgtcggc agcgtgatcg
     2161 ccatcggcgc gcacgtcggc gcttcgagca tgactatcta caacgcactg gcggtgccgg
     2221 tacgcatcga atacggcggc gagcagatcg acgtgcggcc caatcatccg acgacggtga
     2281 ccgtgccggc gggaggccac ctggacgtga gggcggtaac gcaatccagt gagctgatcg
     2341 aagagttcga ggcggacctt cacggcgcta acgccaggta catctacaac gttgccggcg
     2401 cagcgccgct ttatgaatgg accgcggtct acaccccgga aggcgccgga aacacgaacc
     2461 cggccagtgc accctctgaa agacagctcg ggacgcagcg ctggtccacg actgcggtgg
     2521 accacgtgtt cgaagtcccg ccggaacaaa tccagacgag cagcagcagt ggcgcgacct
     2581 atcgcaacgt actgtccgcg gcgagtgatg cctcaccatg gcgccaggtc gagatgacga
     2641 cgaaaccgga ggagaagtcg cagctcgtca tgacgcacgc gcgctgggac aacaccagtt
     2701 cgttgaggat gctcgagtgg ctggatcttg cttcgaagga gccggagttc ggcgcgctgt
     2761 tcgccgcgcg cctgaaacac gagccgaagg acgtgctgct gctgcgctac gaacaggaca
     2821 gcagcgaagg cgagaaacac gacgcggtgt gtgcgcgcca tcgcaaactg gcggagaccc
     2881 agcccgccga ggccaacctg cagtacctgc gcattcgctg cgtgagcgac gaacaggaaa
     2941 agaattcgca gtttgcttcg gcgcgggaga aatggccgga caacccatgg ctgtcgatgg
     3001 ccgccggaat aaccctggcg gagcgcggcc actatctaca ggcgcagccc atgtacgacc
     3061 ttgcgcgcaa acggttgcct gccatgcgcg gctacctggc gctcgaccat gcgcggctgc
     3121 gtcgcctgaa ttcggtggac ggcaaggtcg agcttgccga cctggtcccg aactccgatc
     3181 gcctgggaat gttcgttgca atcgagaccg gccacgacgt cgatgacacg ccgctcgagg
     3241 cctacgcctg gctggcgcgc ggcacgctcg acgtcgccgt gctcaatgca aacaagaaca
     3301 aggaaggccg tgagcgcgta atgcggctgg tggcatcgtc cgacggcgcc acgcgcgcga
     3361 tgatcgacga tgcgctggcg ctgccgctcg acgagaagga cgatttcgaa acggcaatcg
     3421 ccatgtacgc cgtcgcggtc aaggcactgc gcgatccgtc acgccatgtg gcccacctgg
     3481 aaaagatgct ggggccggaa ggcaagccgt tgctggaatt cctggagttc ctgcgacgcg
     3541 gtggagaccc ggtacaggcg cgcaccatgt tgcccgagtt cgatctgcag ctacgcatgc
     3601 acgcgctgca cgcggcggtg atcatgctgg accaccgcgc gccggcggat tggcgcaagg
     3661 aagcctcgcg tggattgttc gtcggcgaac gcggctacct gcgcaatcat tgaggagaag
     3721 gcatcgagtt tcgcaactcg cgcagccttt gacgccacgc gcgcggcgac cccctgttcg
     3781 ggccgcagta ttccgctcgc cggtctcagc cctgacgccg cgcgggcaaa tcagcgtggg
     3841 tccccgacct cggcttcacg cgggtcgcag cgcaacgttc gaccctggcg atcacgtcct
     3901 ttcgccgttc acccgaaagc ggcatgcgcg gcatgcggac gcgctcggag ccacgcccca
     3961 tgatctgctc cgccagcttg atggactgca cgaggtcatg atccgcgtcg aggtgaagca
     4021 gcggcatgaa ccagcgataa atccggcgcg cctcctccca gtcaccgcga tccgccgccg
     4081 cgaccatcgc caccgattct cttggaaacg cactcgtgag tccggacacc cagcccgtgg
     4141 cacccagcag cagcgcctcg agcgcgacgt cgtccagtcc cgccatgagc gtgaatcggt
     4201 ccccaaaacg attgatgatg tcggtgaacc ggcgaggatc tggagcgctc tccttgagcg
     4261 ccacgatgtt ggaaaggggc gccaggcgct ccagtgtttc gagctcgatg gaaacccgat
     4321 acgcgggcgg attgttgtag agcatgatgg gcaacgaggt tgcctcggca atcgcgcgaa
     4381 agtgggcgca caactcctcg ggcgtcggca catagaccat cgccggcagg accatgagcg
     4441 cgtcgatgcc gatcttttcg gcgtcgtgca cgaattcgat ggcgcgatcc gtgctgagct
     4501 ccgacacgcc cgccaccagc ggcacgcgct tgcccacggc ttcgacggcg gcgcgcagcg
     4561 cgaagcgctt ttcttcgggt cgcagcgaat tgttctcgcc cacggttccc agtaggacga
     4621 gcccgtcgac gccgtcatcc accagcgccg tcaccgtctg gcggcacgcg acgatatcca
     4681 gcgataaatc ggcggcaaac tgcgtagtgg cggcgggata aacgccgtgc cataggggct
     4741 tggacaaggg aaatctccac gtctttcagg ttcgtatacg gtatattatt tgtgatgcag
     4801 attgcaaaga actgctcgcc gcacgctgcg gattttccca acgatcgcct ctagtcaacc
     4861 gacgggtttt cacccccatc ggcgatgaat tgcgccatgc gccgttcgag ggcgggcagt
     4921 ggcaccgcgc cgatggcaag aatggcatcg tgaaacttcc gctggtcgaa cttcggaccg
     4981 agcgccgttt ccgcctcgcg gcgatgccgg cgaatctgga gctcacccaa cttgtatgcc
     5041 actgcctgac ccggccaggc aatgtagcgg tccacctcgg tggtgagttc atgttccgat
     5101 agcgcgatgt tgctgcgcat gtagtcgaga gcccgggcac gcgtccaccc gtagtgatga
     5161 atgccggtat ccaccaccag acgcaccgcc ctccacatct cgtaggtgag ctgcccgaac
     5221 tcctcgtacg gcgtctcata gatgcccatg acgttgccca gccattccat gtagaggccc
     5281 cagccctctc cataacccga gaaataggtt tcgttgcgaa acgtcggacg cgcgggcgcc
     5341 tccagcgcca gcgccgcctg gaagctgtga cccggcgtgc attcgtgcag caccagcgac
     5401 ggcagcgtgt acagcggacg agcgctcagg ttgtaagtgt tgaacatgca gctctcgagt
     5461 cctccacgcc ctcccgtgta gataggtgcc agcgcgtccg gcaccggcag gatgccgtga
     5521 cggaagcgcg ggagtgtgcc gatggtttcc ttgagcttga gatcggcctt cttgacgatg
     5581 aaggctgcgt tcgcgagcag ctcgcgcggt gtcctggcat agaactgtgg gtcggtccgc
     5641 aggaacacca tgaattcggg cagcgtgccc ttgaatccgg ccttggccat ggtgtccatt
     5701 tgcagcgccc tgattcgcgc tacttcttcg agcccgatct tgtggatttg ctccgccgtg
     5761 agatcgagcg tggtgaattt ccggatcatc gcctggtagt aggcggcgcc gtccggcaac
     5821 ttcgatgcag ccagtgtctc gcgcgcccgc accaggtatt cggtgcgaat gaaggcaagc
     5881 agcttcgtgt atgccggcac agtgctcctg gcgatggtcc cgcgcgcctc cgtgcgcaac
     5941 cggtcttgct cgtccgcggg catggatgcc ggcattcgca cgaaaggcag atagagtggg
     6001 tttgtgtcat ccgcggcgag ataaggttcg atggtcctgt cgcgaccttg aacggacacc
     6061 ttgggaacgg tgtaaccacg cttgagaccc gcgcgcatgt tgacgatctg ttcgtcgaaa
     6121 taacgcggcg cgtcgcgtaa tcgcccgagg tagcggcgat attcgtccgc cgtcacgaat
     6181 ccctcgcgcg gcgtgaagct ggtccagaag aaagtatccg cgttgaatgg agcctcgtat
     6241 gacttgaagc gggcgtcgac cgcgagctcc tcgactacgg cgcgaaatat ctcgccgttg
     6301 agacgctcgg atgcggacaa cgtcgcgggc tcgatcgcat cgagctgcgt gagcaccgag
     6361 gtccagtacg cgaggcgggc tttctgcgtc gccgcgtcga ctcgcggcaa atggtcggcg
     6421 gtaaaaccgc gcccgacttc gcccgggtgg cgcgcgaatt cctgttgccg ccaatcccat
     6481 tccgcggtgt acagcgcccg caatcgctcg tccgcactcg aagcggactg cgccgccgac
     6541 gggtgagtca tcgacaggca cgccaccgac agcagcatga acgcgatcat ccggacgcga
     6601 ttcgtcatgg gcactccaca tcgag
//
LOCUS       DASQJS010000273        13536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_792689, whole genome shotgun sequence.
ACCESSION   DASQJS010000273 DASQJS010000000
VERSION     DASQJS010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13536
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792689"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="VEW08_10945"
     CDS             <1..908
                     /locus_tag="VEW08_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ41303.1"
                     /translation="GEFRLRLIAAALLALLAATAHAAPPEPTKILVNGVELHYISAGT
                     GEALILLHGGQGDYRSWEPQIAELSRYYRVISYSRRYNFPNQNPPTATDFSVNTEAAD
                     LAALIKALKLKRVNLVGTSMGAATALTMAVENPRLVRSLVLAEPPLLAWARHFPEGPA
                     LYDDFMKRIQDPAREAFAAGNDEAAMRLFVDGFAKPGRFDGLPAEARAGIMQNAGFFR
                     MMSRSENPYPEIDRRALWSLRLPVLVITGEKTIEIHRRIDEELTKAIPRARSASIPNA
                     GHGSPRENPNAFTQVVKNFLETSGK"
     gene            1083..2669
                     /locus_tag="VEW08_10950"
     CDS             1083..2669
                     /locus_tag="VEW08_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ41304.1"
                     /translation="MTEVRPSAPVSLATFAPAYIGPLLREDAVVLRYGAAVVVVLLIA
                     GLRALLAPLLGTQAPLLPFVLGIYAAAYFGGRGPALLASLLTPLLATLWFTSWPDDSP
                     PTQWIAHVVFFVLIALLSSELMHELQKRSRTEREALAIAASHAQALREADRRKDEFLA
                     MLAHELRNPLAPIRNVAYVLTKGAADPATMRRAGQMIERQASHLTHLIDDLLDVARIT
                     RGRVVLKRETTSLDSLVESAIETVQPLLDARVQSVTVRRTAESVFVDGDSVRLCQVLA
                     NLLTNAIKYSPERSRIEIDIEGTAVDAVISVRDPGVGIDAQLLPLLFDPFLQGDRSLD
                     RSQGGLGVGLTIVKLLVEMHGGGVTAHSAGLGKGSEFRIRLPRVFAPIPAAAAPAGDE
                     HKAVRPRRVLVVEDNADSAESMRAVLRTDRHEVEVVYDGAAGLAKLEDFRADIVLLDI
                     GLPRMDGYMVAHAIRERFALAGRRPKLLALTGYGRDDDRNAALRAGFDGHLAKPVDPR
                     LLLQIVADEPQALNTSHSGR"
     gene            complement(2676..4004)
                     /gene="guaD"
                     /locus_tag="VEW08_10955"
     CDS             complement(2676..4004)
                     /gene="guaD"
                     /locus_tag="VEW08_10955"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HYJ41305.1"
                     /translation="MPDTARHHAIRAPALTFTGDPFVEGVEATLRYERDALIAMRDGR
                     ITHFGPADRIRPELPPGIHIKEYGQDSLILPGFIDTHVHYPQTQIIGAYGEQLLDWLT
                     KYTFVAEQQFADAAHARAVAGVFLDECLRNGTTTMATFCTVHAHSVDAFFDAAGKRGM
                     RTIAGKCLMDRNAPETLRDSAQRGYDESKALIQRWHGQGRASYAITPRFAPTSTPQQM
                     ELAGALWKEHPGTYLQSHVAENRAEVAWAKELFPERNGYLDIYDHFGQLGPRAIYGHG
                     IWLTEPELARVHESGTAIAHCPTSNQFLGSGLFDLRNAKLPARPARVGLATDVGAGTS
                     LSMLKTMGAAYEVAQLGGGSLSAALAFYLATRGGAQALYLDDKIGSIAAGMEADLIVL
                     NLKSTPLIEFRMRHCASLEDALFVQMTMADDRAVDTTFVGGVATQRGAAN"
     gene            complement(3997..6315)
                     /locus_tag="VEW08_10960"
     CDS             complement(3997..6315)
                     /locus_tag="VEW08_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ41306.1"
                     /translation="MDSKVFKAGRRPWRSVLSMVNARRSLSGKLMVVMLVTTAIALAS
                     AGAALMYTDLRDNRAAWAEDLRTEAAILALGVTPALSFNDREYAKRSLDALQARDSIR
                     AAALYAADGSVFAQYARADQPGPPATRPQLPGGRSLHIDGEHVLVMKPVVQGNEVLGT
                     IYLSAQYDLSGRVSAYLNVLGAVMIIGLIAALLASSWLHRVVTRPMESMATVAQAIIE
                     KRDYSFRAEKTTDDEVGVVIDAFNKMLDEVQSHARALETSEKLYRAIGESINYGVWVC
                     DAEGRNIYTSDSLLRLVGLTQEQASNSGWGDILHPDDVEETTEAWKECARTGNNWYRE
                     HRMKGVDGRYHAILAQGLPIRDATGRVQRWAGINLDISRLKNTERALLEADRRKDEFL
                     ATLAHELRNPLAPIRNAVRILDSDAADDRQRKWGREVIARQVQRMSLLLDDLLDVSRI
                     TRGQLELKKDYVDLKSVIGIAVETARPLLDAKQHKLVVRLPPENIKLEADPLRLSQVI
                     GNLLANAAKYTDPEGQIALSARIENAELVIAIRDNGIGLTQESLPGLFTMFSQVNSAI
                     DRAEGGLGIGLALVKGLVALHGGRVEARSEGLGRGSEFIVHLPHRVLAPASAMQREEN
                     NGAANASVVRRGRILVADDNRDAAESLALVLRFSGYEVSIAFGGVEALEIGERDRPRA
                     AIIDIGMPGMSGHEVARRMRLEAWGRNAVLIALTGWGQEQDKQAAKAAGFDEHLTKPV
                     DPDDVERVLDELLGDAPAEPGAGSVADSARNA"
     gene            6425..7066
                     /locus_tag="VEW08_10965"
     CDS             6425..7066
                     /locus_tag="VEW08_10965"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HYJ41307.1"
                     /translation="MSRTRNFLTCAIFLVAIAGFCPSLATSAQYSVEAVKAAYLFRFA
                     QYVEWPEATSDAPFIIAVSGADDVAAHLERLLPGMNVNGRRAEVRRVTRVQDLEGVHI
                     LFIGANTFGRTRALRTRAIEKPILIVTESEDGIDGGAVINFIEVDRKLRFEISLNAAD
                     RSGLRINSALLSVAARVERRPQGALNCPDGNGRRQRLHGCRIRLAGAGFRRGA"
     gene            7063..8979
                     /locus_tag="VEW08_10970"
     CDS             7063..8979
                     /locus_tag="VEW08_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ41308.1"
                     /translation="MKVLSTRICIALVVAASSPAGAQLVASASDPLTELSRMSLEQLA
                     NVEVTSVSKSAQPLSSAAASIYVITREEILRSGVLSIPEALRLAPNLQITQLTSTEYS
                     NGARGFAGAPDVQNFSNKILILIDGRSVYSPLFSGVAYDMLDVVMDDIDRIECISGPG
                     ATLWGANAMNGVINIITRNASDTRGTLVRLDGGPEEQAAALRYGGDVGEDAAFRVYAK
                     YFDRGATELGNGDGAGNDWNKLQAGFRMDGGSGRNHFTVQGDYQTADQSFTGTPDVPF
                     SGANLLGRWEHSGDRLNTRVQVFFDRVDRDRPPDGLAFDIDTYDFDLQQSATVGARHN
                     LVWGLGRRSNDYHTQNNNLAFVPDHRTLELTNVFLQDSITLNEELKLTAGIKFEDNSY
                     SDWSVLPDLRLSWAPNDKTLFWLAGSRAIRAPTPFDTDVQEFVGGQLFVLGDPDFKTE
                     KVTAYELGYRGNPDARLSVSASVFYNEYDDLRTIEVTPVVFLPLRWGNLMEGSAYGFE
                     AWADIQLNSWWRISPGVRTLHKRLEFSEGASQIVGVRQAGNDPSSRYYLKSSMDLRRF
                     TFDVMVRKVSELPDPALDDYTELNARVAWRATEKLELAVKGFNLLNETHLEYPDPAGQ
                     AIRRSVMAELRYNY"
     gene            9059..9517
                     /locus_tag="VEW08_10975"
     CDS             9059..9517
                     /locus_tag="VEW08_10975"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HYJ41309.1"
                     /translation="MSAPMQQRRATDDRRRSVFHALWHGNFARRRHAPRRRDERHVVS
                     TDWFQAQWLAVGFGILLLCAADALLTLTLISHGAVEINPLMDPLVRGSGHSFGYWKTG
                     LTSMGVLVLTLLARVRFWGRTVGTILYVVLGAYAVLVAYEVFLLRNIPLD"
     gene            9610..12438
                     /locus_tag="VEW08_10980"
                     /pseudo
     CDS             9610..12438
                     /locus_tag="VEW08_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
     gene            12544..>13536
                     /gene="sucB"
                     /locus_tag="VEW08_10985"
     CDS             12544..>13536
                     /gene="sucB"
                     /locus_tag="VEW08_10985"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR01347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HYJ41310.1"
                     /translation="MSIEIKVPQLPESVADATLVTWHKQVGQAVTRDENLADLETDKV
                     VLEVPAPVSGVIKELRIVAGTSVTSGQVLAVLEEGGVATAAPAKAAAASAPAAAKAAP
                     VATPAAANDNGKLAPSVRRMVEEKQLDPSKIPATGRDGRITKGDVVEYLDKPAAKATG
                     GDKPAAKPAAPAATAGAAGRQDQRVPMTRLRARIAERLLEAQHSQAQLTTFNEIDLKA
                     VMDLRAKYKDGFEKKHGVKLGFMSFFVKAAVEALKRFPTVNASIEGSDIVYHEYYDIG
                     VAVSTEKGLMVPVLRDVDLQGYADIEKNIGAFATRARDGKITIEELTGGTFSITN"
BASE COUNT         2383 a         4323 c         4343 g         2487 t
ORIGIN      
        1 tgggagaatt ccgcttgaga ttgattgcgg ccgcattgct ggccttgtta gccgcgacgg
       61 cgcacgccgc gccgcccgag cccacgaaaa tcctcgtcaa tggcgtcgag ctgcattaca
      121 tcagcgccgg caccggcgaa gcgctgatcc tgctgcacgg cggacagggt gactatcgct
      181 cgtgggagcc gcagatcgcc gagctttcgc gctactaccg cgtcatctct tacagccgcc
      241 gttacaactt tcccaaccag aacccgccga cggccaccga cttttcggtg aatacggagg
      301 ccgcggatct cgcggcgctc atcaaggcgc tgaagctgaa gcgcgtgaat ctcgtcggca
      361 cgtcgatggg cgccgcgacc gcgctgacga tggcagtcga aaatccgaga ctggttcgca
      421 gcctggtgct tgccgagccg ccgctgctcg cgtgggcgcg gcattttcct gaagggcccg
      481 cgctgtacga cgatttcatg aagcgcatcc aggacccggc acgcgaggct ttcgctgccg
      541 gcaatgacga agcggccatg aggttgttcg tcgatggctt cgccaagccc gggaggttcg
      601 acggtctgcc ggcggaggcg cgggcgggca tcatgcagaa cgcgggattt ttcaggatga
      661 tgtcgcgcag tgagaatccg tacccggaga tcgaccggcg agcgctgtgg tcgctgcgtc
      721 tgccggtgct cgtgatcacg ggcgagaaga ccatcgagat ccatcggcgt atcgatgaag
      781 agttgaccaa ggcaatcccg cgcgcgcggt cggccagcat tcccaatgcg gggcatggat
      841 cgccgcgtga gaatccgaac gcatttacgc aagtggtcaa gaactttctg gagaccagcg
      901 gtaaatgaga accggctcct ccgaagacct ggagcgggtt cggctcaacg acaagcttgt
      961 tgggcaaacc aggcgccgga aaattcctac attttgctga gagcgactct tagtgtcagc
     1021 ggcgctagag tccgcgacga cggtcacgca ggcatttgcg attagcgatc agcaaggaat
     1081 tgatgaccga ggttcgccct tccgcaccgg tttcactggc tacgtttgct cctgcctata
     1141 ttggcccgct gctgcgtgaa gacgccgtag tgctccgata cggcgcggcc gtggtcgtgg
     1201 tgctgctgat tgccggtctt cgcgcactgc tcgcaccatt gctaggtacg caggcgccgc
     1261 tgttgccgtt cgtcctgggg atatatgcgg ccgcctactt cggcggacgc ggacctgcgc
     1321 tcctggcaag cctgctcacg ccattgctgg ccacactgtg gttcacgagc tggccggatg
     1381 actcgccgcc cacccagtgg attgcacacg tagtgttctt cgtgctgatc gcgctgctct
     1441 cgagtgagct catgcatgag ttgcagaagc ggtcgcggac ggagcgcgaa gcgctggcca
     1501 tcgccgcgag ccacgcgcag gcgctgcgtg aagccgaccg ccgcaaggac gagttcctcg
     1561 ccatgctggc gcatgaactg cgcaatccat tggcgccgat ccgcaatgtc gcctacgtgc
     1621 tcaccaaggg cgccgccgat ccggccacga tgcgccgcgc gggacagatg atcgagcggc
     1681 aggccagcca cctcacgcac ctcatcgacg acctgctcga tgtcgcgcgc atcacgcgcg
     1741 gccgggtggt gctcaagcgt gaaacgacga gcctggattc cctggtcgag tcggcgatcg
     1801 agaccgtgca gccattgttg gatgcgcgcg ttcagtccgt gacggtgcgg cgaactgcgg
     1861 aaagcgtgtt cgtcgatggt gacagcgtcc gcctctgcca ggtgcttgcg aaccttctga
     1921 cgaatgcgat caagtactcg cccgaacgct cgcgcatcga aatcgacatc gagggcacgg
     1981 cggtcgatgc ggtcatcagc gtgcgcgatc cgggcgtcgg catcgacgcg caactgctgc
     2041 cgctgctgtt cgatccattc ctgcagggcg atcgctcgct cgaccgctcg cagggcggtc
     2101 ttggcgtggg gctcacgatc gtcaaactat tggtcgaaat gcacggcggc ggtgtgacgg
     2161 cgcacagtgc gggcctgggc aaaggcagcg agtttcgcat ccgcctgccg cgggttttcg
     2221 caccgattcc agcggccgcg gcgccggcgg gcgacgagca taaggccgtg cgtccgcgcc
     2281 gcgtgctcgt cgtcgaggac aacgcggact cggccgaatc catgcgcgcg gtgttgcgca
     2341 cggatcgtca tgaagtggaa gtcgtgtacg acggtgccgc gggcctggcg aagctcgaag
     2401 atttccgtgc ggatatcgtg ctgctcgata tcggcttgcc gcgcatggac ggctacatgg
     2461 tggctcacgc catccgcgag cgtttcgcgc tggcggggcg ccggccaaaa ctgctggcgc
     2521 tcacgggcta cggacgcgac gacgatcgca atgccgctct cagggcgggc tttgacggac
     2581 acctggcaaa acccgtggat ccgcgccttc tcctgcagat cgtggccgac gagccgcagg
     2641 cgttgaatac gagccattcg ggccgatagg cgccctcaat tcgccgcgcc gcgctgggtg
     2701 gccacgccgc cgacgaaggt ggtgtcgacc gcacgatcgt cggccatcgt catctgcacg
     2761 aacaaggcgt cttcgagcga agcgcaatgt ctcatccgga attcgatgag cggcgtggac
     2821 ttgaggttca gcacgatcag atccgcttcc ataccggcag cgatactgcc gatcttgtcg
     2881 tcgaggtaca gcgcctgcgc gccgccgcgg gtcgcgagat agaacgcgag cgcggcggac
     2941 aaactgccgc cgccgagctg cgccacctca tatgccgccc ccatggtctt gagcatggac
     3001 aacgacgttc ccgcgccgac atcagttgcc aggccgaccc gcgccggccg tgcgggtagt
     3061 ttggcattgc gtaaatcgaa gagtccgctg ccgaggaact gattggaggt ggggcagtgt
     3121 gcgatggcgg tgccgctctc gtgaacacgg gcgagctccg gctcagtcag ccagatgccg
     3181 tgcccataga tggcgcgtgg gcctaactgc ccgaaatggt cgtagatatc gagataaccg
     3241 ttgcgctcgg gaaacagttc cttcgcccat gcgacctcgg cacggttttc ggcaacgtgc
     3301 gactgcaggt aggtgccggg atgttccttc cacaaagcgc cggccagctc catctgctga
     3361 ggagtgctgg tgggggcgaa gcgcggcgtg atggcgtatg aggcgcgccc ttggccgtgc
     3421 cagcgctgaa tcagcgcctt cgactcatcg tatccgcgct gtgcgctgtc acgcagcgtc
     3481 tcgggcgcgt tgcggtccat gaggcacttg ccggcgatcg tgcgcatgcc gcgtttgccc
     3541 gcggcgtcga aaaatgcatc caccgagtgc gcatgcacgg tgcagaacgt ggccatggtc
     3601 gtggtgccat tgcgcaggca ctcgtcgaga aaaacgccgg cgacggcgcg ggcgtgcgcc
     3661 gcgtccgcga actgctgttc ggcgacgaaa gtgtatttcg tgagccagtc gagcagctgc
     3721 tcgccgtagg cgccgatgat ttgcgtctgc gggtagtgaa catgcgtgtc gatgaagccg
     3781 ggcaggatca gggagtcctg gccgtactct tttatatgta tacccggagg cagttcgggg
     3841 cggatgcggt cggcgggccc gaaatgcgtg atcctgccgt cgcgcatggc gatcaacgca
     3901 tcgcgctcat aacgcagcgt ggcttcgacg ccttctacga aggggtcgcc ggtgaatgtg
     3961 agcgcgggtg cgcggatggc gtggtgtctg gccgtgtcag gcattgcggg cagagtctgc
     4021 cacactgccg gcgccaggct ccgccggcgc atcacctaac agttcgtcga gcacacgctc
     4081 gacatcatcg ggatccacgg gcttggtcag gtgttcatcg aacccggcgg ccttggcagc
     4141 ctgtttgtcc tgctcctgac cccagccggt gagcgcgatg agcacggcat tgcgtcccca
     4201 ggcttcgagg cgcatgcggc gcgccacttc gtgtccgctc atgcccggca tgccgatgtc
     4261 gatgatggcc gcgcgtggcc ggtcgcgttc gccgatctcc agcgcttcta cgccaccgaa
     4321 cgcaatggaa acttcgtagc ccgagaagcg cagcaccagc gccagggatt ccgcggcgtc
     4381 gcgattgtcg tcggcgacca ggatgcggcc gcgccgcacg acgctggcat tcgcggcgcc
     4441 gttgttttct tcgcgttgca tggcgctggc gggagcgagc acgcggtgtg gcaggtggac
     4501 gatgaattca ctgccgcgac ccaatccttc gctgcgcgcc tcgacgcgac caccgtgcag
     4561 cgccaccagt cctttgacca gtgcgagacc gatgccaagg cctccctcgg cgcgatcgat
     4621 ggcggagttc acctgcgaga acatggtgaa caggcccggc agcgattcct gcgtgaggcc
     4681 gatgccgttg tcgcgaatgg cgatgaccag ttccgcattc tcgatgcgcg ccgacaacgc
     4741 gatctgtcct tccgggtccg tgtacttggc ggcgttggcc aacaggttgc cgatgacctg
     4801 cgacaggcgc agcggatcag cctcgagctt gatgttctcc ggcggcaggc gcacgacgag
     4861 tttgtgctgc ttggcgtcga gcagcgggcg cgcggtttcc acggcgatgc cgatcacgga
     4921 tttgaggtcg acgtagtctt tcttgagttc gagctggcct cgcgtgatgc gcgacacgtc
     4981 gagaaggtcg tcgagcagca gcgacatgcg ctgcacctgg cgcgcaatga cttcgcggcc
     5041 ccacttgcgt tgccggtcat ccgcggcgtc cgaatcgagg atgcgcaccg cattgcgaat
     5101 gggcgcgagc ggattgcgca actcgtgcgc gagtgtggcc aggaactcat ccttgcgccg
     5161 gtcggcctcg agcagtgcgc gttcggtgtt cttgaggcgg ctgatgtcga ggttgatgcc
     5221 ggcccagcgc tgcacgcgcc cggtcgcatc gcgaataggc aggccctggg caaggatggc
     5281 gtggtaccgg ccgtcgacac ctttcatgcg gtgttcgcga taccagttgt tgccggtgcg
     5341 cgcgcattct ttccatgcct ccgtggtttc ttcgacgtcg tccggatgca ggatatcgcc
     5401 ccagccggaa ttcgacgcct gttcctgggt cagtccgacc aggcgcaaca gcgaatcgct
     5461 ggtgtagata tttcggccct cggcatcgca gacccacacg ccgtagttga tggactcgcc
     5521 gatggcccgg tacagcttct cgctggtctc gagcgcgcgc gcgtgcgact gcacctcgtc
     5581 gagcatcttg ttgaacgcgt cgatgaccac gccgacctcg tcgtcggtgg tcttctccgc
     5641 gcgaaacgaa tagtcgcgtt tttcgatgat ggcctgtgcg accgtggcca tggactccat
     5701 cggccgggtg accacccggt gcagccagct cgacgcgagc agggcggcga tgaggccgat
     5761 gatcatcacc gcgccgagca cgttcagata cgcgctcacg cgccccgaca gatcgtattg
     5821 cgcgctcagg tagatagtgc ccagcacttc gttgccctgg accacgggct tcatgaccag
     5881 cacgtgttcg ccgtcgatgt gcaggcttct gccacccggc agttgcggcc gggtcgccgg
     5941 gggacccggt tgatcggcgc gcgcgtactg ggcgaacacg ctgccgtcgg ccgcgtagag
     6001 cgccgccgcg cgaatggaat cgcgtgcctg cagcgcgtcc aggctgcgct tcgcgtattc
     6061 gcggtcgttg aacgaaagcg cgggtgtcac gcccagcgcg agaatggccg cttccgtgcg
     6121 caggtcttcg gcccaggcgg cgcggttgtc gcgcaggtcg gtgtacatga gcgcagcacc
     6181 cgcggaggcg agcgcgattg ccgtggtgac cagcatcacc accatgagct tgccggagag
     6241 acttcggcgt gcattcacca tggaaagcac cgaacgccac ggacggcggc cggctttgaa
     6301 aactttacta tccatcgagg atcccacggg gacgcatctt gccttcatat cggaaatacg
     6361 ttccactgca atcggccttg tcctacaaaa acaactcgga gctgtgcagt tgccgcaccg
     6421 gcgtttgagc cgcactcgaa acttcctgac gtgcgccatc tttctggtcg ccatcgccgg
     6481 cttctgtccc tcgttggcga catccgcgca gtactcggtc gaagccgtca aggcggccta
     6541 tctgtttcgc ttcgcgcagt acgtcgaatg gcccgaagcg acctccgatg ctccattcat
     6601 catcgccgtc agcggcgcgg atgacgtcgc cgcacacctc gagcgcctgt tgccgggcat
     6661 gaacgtcaac ggccggcggg ccgaggtgcg tcgcgtgacg cgcgtgcagg atctcgaggg
     6721 cgtccacatc ctgttcatcg gcgcgaacac attcggtcga acgcgcgcgc tccgtacacg
     6781 cgccatcgag aagcccattc tcatcgtcac ggaaagcgag gacgggatcg atggtggcgc
     6841 cgtcatcaat ttcatcgaag tggatcgcaa gctgcgtttc gaaatttcat tgaacgcggc
     6901 ggatcgcagc ggccttcgaa tcaattcggc cttgctttcc gtggccgcgc gcgtcgagcg
     6961 ccggccgcag ggagcgctca attgtcccga cgggaatggg cgccgacagc gcctccatgg
     7021 gtgccgaatc aggttggccg gcgcaggttt caggagaggg gcgtgaaggt cttgtcgacg
     7081 cgtatctgta ttgccctggt cgtggccgcc agttcgccgg ccggggcaca gctggtggcc
     7141 agcgccagcg atccgctgac cgagctttcc cgcatgtcgc tcgagcaact tgcgaatgtc
     7201 gaagtcactt cggtgtccaa gtccgcacag ccgctctcga gcgccgcggc gtccatctac
     7261 gtcatcacgc gcgaagaaat cctgcggtct ggggtgctca gcattcccga ggcgttgcgc
     7321 ctcgcgccca acctgcagat cacccagctc acctcgaccg aatattccaa tggcgcgcgc
     7381 ggtttcgccg gcgcgcccga cgtgcagaac ttctccaaca agatcctcat cctcatcgac
     7441 ggccgcagcg tctattcacc gctgttctcc ggcgtcgcct acgacatgct cgacgtggtc
     7501 atggacgaca tcgaccgcat cgagtgcatt tcggggcctg gcgcgaccct gtggggcgcc
     7561 aatgccatga acggtgtcat caacatcatc acgcgcaatg cctcggacac gcgcggcact
     7621 ctcgtgcggc tggacggcgg ccccgaggaa caggccgcgg cgctgcgcta cggtggcgat
     7681 gtgggcgaag acgccgcatt ccgcgtctac gcgaagtact tcgatcgcgg cgccaccgaa
     7741 ctcgggaacg gtgacggtgc cgggaacgac tggaacaagc tccaggcggg ctttcgcatg
     7801 gacggtggat ccggccggaa ccacttcacc gtccagggcg actatcagac cgccgatcaa
     7861 tccttcaccg gcacccccga cgtgccgttc tccggcgcaa atttgttagg gcggtgggaa
     7921 cattcgggcg accgcctgaa tacacgtgtc caggtgttct tcgatcgcgt cgatcgcgat
     7981 cggccgccgg acgggctggc gttcgacatc gacacctacg atttcgatct gcagcaaagc
     8041 gccaccgtcg gcgcccggca caacctggtc tggggactgg gccggcgttc caacgactac
     8101 cacacgcaga acaacaacct cgcgttcgtg cccgaccacc gcacgctcga gctcacgaac
     8161 gtgttcctgc aggactccat cacgctcaac gaagagttga agctcaccgc cggaatcaag
     8221 ttcgaggaca acagctattc ggactggagc gtgttgccgg acctgcggct ttcctgggcg
     8281 ccgaatgaca agacgctgtt ctggctcgcc ggttcgcggg ccattcgcgc gcccacgccg
     8341 ttcgacaccg acgtgcagga gttcgtcggc gggcagctct tcgtgctcgg cgatcccgat
     8401 ttcaagaccg aaaaggtaac ggcatacgag ctcggttatc gcggcaatcc ggacgccagg
     8461 ctgtcggtgt cggcgagtgt tttctacaac gagtacgacg acctgcgcac catcgaggtg
     8521 acgccggtcg ttttcctgcc gctgcgctgg ggcaatttga tggaaggcag cgcctatggc
     8581 ttcgaagcct gggctgacat tcagctcaac tcgtggtggc gcatttcgcc gggcgtgcgc
     8641 accttgcaca agcgcctgga gttcagcgag ggcgcgtcgc agatcgtggg cgtgcgccag
     8701 gcgggcaacg acccgtcctc acgctactac ctcaagtcct cgatggacct gcggcgtttc
     8761 accttcgacg tgatggttcg caaggtcagc gaacttccgg atccggcgct cgacgactac
     8821 accgaactga atgcgcgcgt cgcatggcga gcgaccgaga agctcgagct cgctgtcaag
     8881 gggttcaatc tacttaacga aacacacctc gagtacccgg atcccgcggg ccaggcgatc
     8941 cgccgcagcg tcatggcgga gctgcgctac aactactgac cgaattggga cttggctcac
     9001 acgcattcgc ggcggccggg cgttcacgtc ggtcgccggc tcatagaatg attggcagat
     9061 gtcagcacca atgcagcaac ggcgcgccac ggatgatcgg cgccgttcgg ttttccacgc
     9121 cctgtggcac ggcaacttcg cccgcaggcg gcatgcccca cgtcgacgcg acgagcgaca
     9181 tgtcgtcagc acggactggt ttcaagccca atggctcgcc gttggctttg gcatccttct
     9241 gctgtgcgct gccgacgcac tgctgacgct gacactcatt tcccacggcg cggtcgagat
     9301 caatccgttg atggacccgc tggtgagggg atctggccat agttttggct actggaagac
     9361 cgggctcacc tcgatgggtg tgctggtgct cacgctgctg gctcgtgttc gtttctgggg
     9421 cagaacggtc ggaacgatcc tttatgtggt tcttggggca tacgcggtcc tcgtcgctta
     9481 cgaggtattc cttttacgta acattcccct agattaggta ctggtcgcct ttcgggcagg
     9541 ggcgccttcg gctaccatac cgccccctcc atagctccag gaccttgagt ccacaggatt
     9601 ttttctcgta tggccccaag ccttcgcgag ctttcggctt ccacgccgct gtctggcggc
     9661 aacgctcctt acgtcgaagc tctttatgag cagttcctct ccgatccgca cagtgtcgat
     9721 ccggcatggc gtgattactt tcagcgtctc caggatggcc tgaagcaacg cgatggcgcg
     9781 ggagctgaac agattcactc caccgtgcag gccggcatcg tgcagcgcgc gggcaaaccg
     9841 cgcgtggccg cgggcggctc aggcacgctg agtgccgatg ccgccgccaa gcaaggctcg
     9901 gtgtcgcggc tggtacagat ctacgccaac cgcggccacc tcatcgccaa cctcgatcct
     9961 ctggggctcg aaaaacgcga gcgtccccga gtgctcgagc tcgggtattt cgggctgacg
    10021 gatgccgacc tcgacaccga gtttcttacc ggttcgcgcc ccaatgagct ggcgcccagg
    10081 ctcaagctgc gcgagatcat cgcgcaactc gaacacgcgt actgcggcac catcggcgcc
    10141 gagttcgcgc acgtgtcgaa caccgaagag cgactgtggc tgcaggacca gtacctggtc
    10201 gggcggctgc gcggcaaact caatgtcgac gagcagaaaa acattctctg gcagctctcg
    10261 gcggccgaag gcctcgagcg ttatctgcac accaagtacg tcggccagaa gcgtttctcg
    10321 ctcgaaggcg gcgactctct cgttccgctc atggacgatc tcatccagcg tggcggcgaa
    10381 cagggcgtcg aagagtgcgt gatcggcatg gcccatcgtg gccgcctcaa cgcgctggtg
    10441 aacctgctcg gcaagtcgcc ctcggtgctg ttcaacgaat tcgaaggcaa gtacgacacc
    10501 gcgcacatgc acggctcggg tgacgtgaag taccacaagg gcttctcgtc ggatctgcgc
    10561 acgcccggcg gcaatgtgca cgtggcgctg gcgttcaatc cttcgcacct cgaggtcgtg
    10621 aatccggtcg tcgaaggttc ggtgcgcgca cgccaggaac gccgcgggga ttccgccggc
    10681 acccgcgtgt tgccgatcct ggttcacggc gatgcggctt tcgcgggcca gggcgtcgtc
    10741 atggaaacgc tgcagctgtc gcaggcgcgc gcgttcttca ccggtggcag cgtccacatc
    10801 atcatcaaca accaggttgg cttcaccacg tcggatccgc gcgatgcgcg ttcgacgatg
    10861 tattgctcgg acgtcgccaa gatgctcgag acgccgatct ttcacgtgaa cggggacgat
    10921 cccgaagcgg tggtgttcgt gacacgcctc gcgcttgcgt atcgccagcg cttccacaag
    10981 gacgtggtca tcgatctcgt ctgttaccgc cgccatggtc acaacgaggc ggatgagcca
    11041 tcggccacgc agccgatgat gtaccagatc attcgcaagc atccgacgac gcgcaagctc
    11101 tacgccgaca aactcgtggc gcagggtgtc atcaccgacg gcgatgcggc ggcaatgatc
    11161 gagtcatatc ggcagggcct cgacgaaggc cggccgcagg cgcgcgcctc gctcggcatg
    11221 atcggcaaca agtacacggt cgactggagc aagtacctcg gcaacgactg gaccgagcgc
    11281 gtgaagggtg gcggcaacat ggcgcgcctc aagtcgctcg gcgaacgcat cgtgaagttc
    11341 ccggagaatt tctcgctgca tccgcgcgtg gcgcaggtca tggccaaccg cgtgaagatg
    11401 ctcgccggcg acctgccgct cgactggggc tttgccgaga cgatggccta cgccagcatt
    11461 ctcgaagagg gaaattccat ccgcttcacc gggcaggacg ccggtcgcgg tacgttcttc
    11521 catcgccacg ccgtgctgca cgatcaggcc acgggcgcgc gttacattcc gctgcagcac
    11581 atcgccgagc gtcagccttc gttccagatc atcgactcgg tgttgtcgga agaggccgtg
    11641 ctcggattcg aatacggtta ctccaccacc gatccgtcgg cgctggtcat ctgggagggg
    11701 cagttcggcg atttcgtcaa cggcgcccag gtcatcatcg atcagttcat cagctcgggt
    11761 gaagccaagt ggggccgcct ctgcggtctc acactgttcc tgccgcatgg ctacgaaggc
    11821 caggggccgg agcattcctc cgcgcgtctc gagcggttcc tgcagctctg cgccgagaac
    11881 aacatgcagg tgtgcgtgcc ttcgacgccg gcgcagatgt atcacatgat ccgccggcag
    11941 atcctgcgca gcttgcgcaa gccgctcatc gtcatgacgc cgaagagcct gctgcgtcac
    12001 gagctgtcgg tgtcttcgct cgaagacctg gccaatggcg agttcgccac ggtcatcgac
    12061 gaaatcgaca aggtgactcc tgccaatgtg cgccgcgtcg tgctgtcgtc agggaaggtt
    12121 tacttcgacc tgctgaaggc gcgccgcgct tccaacaatt ctgaagtggc gctggtgcgc
    12181 gtcgaacagc tctatccatt cccggtgccg gaaatggaag cggtgctcgc gaaatatccc
    12241 aacgctcgcg aagtggtgtg gtgccaggaa gagccgcaga accagggcgc gtggtaccag
    12301 atccgccacc gcctggccac gctcgtcggc aagcgcgaac tgctgtacgc aggccgtgca
    12361 ccggccgctg cgccgtccac cggcatcacc aagatccacg aagccgaaca gcgcgggctc
    12421 atcgaagccg cgttgactgc caccaacaag gaagacgggg cgcgcgaaac caagcgcctg
    12481 atgtcgacac cggctccggc cgctgccgcc acctcccgtt aagagaacga gcaagggaag
    12541 tccatgtcca tcgaaatcaa ggttccgcaa ttgcccgagt cggtcgccga cgcgacgctg
    12601 gtcacctggc acaagcaggt tggccaggcc gttacgcgtg acgagaatct cgccgatctc
    12661 gagaccgaca aggtggtgct cgaggttccg gcgccggtaa gcggcgtgat caaggaactg
    12721 cgcatcgtcg ccggcacttc ggtgaccagc ggacaggtgc tcgcggttct cgaagaaggt
    12781 ggcgtggcca ctgcagcgcc tgccaaggcc gccgcagcct cggctcccgc cgcggccaag
    12841 gctgcgcccg tggcgacgcc cgcggccgcg aacgacaacg gcaaactcgc gccgtcggtg
    12901 cgccgcatgg tcgaagagaa gcagttggac ccgtcgaaga ttcccgccac gggccgtgat
    12961 ggtcgcatca ccaagggtga tgtcgtggaa tatctggaca agcctgccgc caaagcgacg
    13021 ggcggcgaca aaccagccgc caagcccgct gctccggccg ccaccgcggg cgccgccggc
    13081 agacaggacc agcgcgtgcc gatgacgcgt ctgcgcgcgc gcatcgccga gcgcctgctc
    13141 gaagcgcagc actcgcaggc tcagctcacc acgttcaacg agatcgacct caaggccgtg
    13201 atggacttgc gggcgaaata caaggatggc ttcgagaaga agcatggtgt gaagctgggc
    13261 ttcatgtcct tcttcgtgaa ggctgcggtc gaggcactca agagattccc gaccgtcaat
    13321 gcctcgatcg aaggcagtga catcgtgtac cacgagtact acgacatcgg cgtcgcggtc
    13381 tccaccgaga aggggctcat ggttcccgtg ctgcgcgacg tggatctgca gggctacgcc
    13441 gacatcgaga agaacatcgg cgcgttcgcc acgcgcgctc gtgatggcaa gatcaccatc
    13501 gaagagctca ccggcggcac gttctccatc accaac
//
LOCUS       DASQJS010000274        12104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1734973, whole genome shotgun sequence.
ACCESSION   DASQJS010000274 DASQJS010000000
VERSION     DASQJS010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12104
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734973"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="VEW08_10990"
     CDS             complement(<1..171)
                     /locus_tag="VEW08_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYJ41311.1"
                     /translation="MSKEKFERNKPHVNVGTIGHVDHGKTTLTAALTKVMAETHGGSY
                     MAYDQIDKAPEEK"
     gene            complement(230..305)
                     /locus_tag="VEW08_10995"
     tRNA            complement(230..305)
                     /locus_tag="VEW08_10995"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(270..272),aa:Thr,seq:ggt)
     gene            complement(310..383)
                     /locus_tag="VEW08_11000"
     tRNA            complement(310..383)
                     /locus_tag="VEW08_11000"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(349..351),aa:Gly,seq:tcc)
     gene            complement(483..568)
                     /locus_tag="VEW08_11005"
     tRNA            complement(483..568)
                     /locus_tag="VEW08_11005"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(532..534),aa:Tyr,seq:gta)
     gene            complement(717..1256)
                     /locus_tag="VEW08_11010"
     CDS             complement(717..1256)
                     /locus_tag="VEW08_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41312.1"
                     /translation="MKNGFLLPIVTLAISLASPVHADVKETRENGFVIEATVMADANP
                     AAVYRQLIRVADWWDPKHTWSGSARNLKLEAKAGGCFCEKLADGGSVQHARVIFAQPG
                     KLLRLDGALGPLQDMAVSGVLSFNLAPDGPGTRINMTYRIAGVLAMDSAKLAPLVDQV
                     MGIQLGRLRSFASGAPAGP"
     gene            complement(1253..1999)
                     /locus_tag="VEW08_11015"
     CDS             complement(1253..1999)
                     /locus_tag="VEW08_11015"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HYJ41313.1"
                     /translation="MTERLQKLLAGAGHGSRRGIEEWIRAGRVTINDRVAVLGDRASD
                     ADRICLDGKPLDLGGRRESVEVLLYHKPVGEVTTRADPENRPTVFERLPEPSSGRWIV
                     VGRLDVNTSGLLLFTNDGDLAHRLMHPSSEIRREYLVRLRGSPPAEVLERLRRGVELE
                     DGEANFDEIHVQSVEGSHTWLRVCLHEGRNREVRRLFEHEGYIVSRLTRLRYGSVELP
                     KDLPGGAFKALPRPAITELSNLAFAGSESP"
     gene            2087..3028
                     /gene="cysK"
                     /locus_tag="VEW08_11020"
     CDS             2087..3028
                     /gene="cysK"
                     /locus_tag="VEW08_11020"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HYJ41314.1"
                     /translation="MLYDNILGTIGRTPIVRINRLAPAGTTMYVKCEFFNPLSSVKDR
                     LAIAIIEDAERSGTLKPGQTVVEATSGNTGIALAMVCAAKGYPFVAVMVETFSIERRK
                     IMRMLGAKVILTPAAERGQGMVRKAAELAEKHGWFLARQFENPANPAYHRNTTGPEII
                     QDFVGKRLDNFVTGWGTGGTLTGVGEVLKLTRPDVKVIVSEPEAAQLLGGKPFAPHKI
                     QGWTPDFVPAVLNRNVADRIVPVTDVEAIESARALARTEGIFVGISSGATFAAALKVA
                     REAGPGSVTLAMLPDTGERYLSTALFEGISDGGDPEP"
     gene            3025..4221
                     /locus_tag="VEW08_11025"
     CDS             3025..4221
                     /locus_tag="VEW08_11025"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HYJ41315.1"
                     /translation="MSGQPREKFGHADSKEMNRAVAAELAEVSLGPATKFAALPDPFR
                     FWRGGALSEGRVAYETWGSLNAARDNAVLLFTGLSPSAHAASSPEDPSSGWWEKMVGP
                     DKAIDTNRYFVVCVNSLGSCFGSSGPASIDPATGLAYRLDFPDLSIEDIARGGYETIR
                     SLGIDRLSAVVGASLGGMAVLAFAVQFAGVARRVISISGSPAAMPFAIALRSTQRDAI
                     LTDPDWQDGQYTGDVRPITGMRLARKLGTLTYRSAAEWKHRFGRRPASDRRYETLSPG
                     RAHFPAEFAVQDYLNAQADKFVSVFDPNCYLYLSRAIDRFDLSAHGGTPVAALKRAAA
                     ERALIIGVESDILFTINEQNALAEAFTNAGTATDFVPLASLEGHDAFLVDIEPFAAAI
                     GKFLGE"
     gene            4317..4520
                     /locus_tag="VEW08_11030"
     CDS             4317..4520
                     /locus_tag="VEW08_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41316.1"
                     /translation="MKHSNLAERLEEFVAALSVIDQPEPDTNTTRILAGVARPAKQAP
                     RRLSLTARHIQIRDQGLRPFRVS"
     gene            complement(4517..6442)
                     /locus_tag="VEW08_11035"
     CDS             complement(4517..6442)
                     /locus_tag="VEW08_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYJ41317.1"
                     /translation="MDFYARQEAARRTTRWLLVAFLISVVLVVAAVDAVVAFVIASSD
                     PGTSPVNAVVGSSIVVLGIICGASLFKTLSLRAGGGVVARSLGGTRIERGTGDIALKR
                     VHNVVEEMSIASGVTMPEVYVLEHEDGINAFAAGNSPADAAIAVTRGAATRLKRDELQ
                     GVIAHEFSHILNGDMRLNVRLLGWTFGLLAVAIVARVVLQSSPGSGGRSRKDGAGALM
                     IAALAAMVLGYIGVFFGRLLQAAVSRHRERLADASAVQFTRNPVGLTGALLKIAGAGA
                     GSRLVTPEAEEVAHMLFAAGLPRLFSTHPPLEERLKALDPSFRASELPKLAADAARDS
                     ERQRAEDATRTPQSQRHESSASAFTPAGDRSIRGHAAHDIAALAGTIANENVRYAENA
                     RVAIPEALRDFVDSADHARALMLVVLASRIPEVQAEQRRILEAVYGPEFTARVFGMQT
                     LADSLAPALRLPAVQQLFPALRRLSLTERQRLRDVVAQLANADARIDVFECCLTLLLA
                     SNLDDLEAGPMHGSESLMQEIPAIHALFAILAAQGTGDDARARRAYEAGISVVLPEHR
                     PAYEQIARWPKALADSLARLANLRPFAKKVLIEGLVRCIAHDQKLSVEEGELLRTVCS
                     VLHCPLPPILSAPAPGS"
     gene            complement(6446..7030)
                     /locus_tag="VEW08_11040"
     CDS             complement(6446..7030)
                     /locus_tag="VEW08_11040"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HYJ41318.1"
                     /translation="MGWILLALVVVAIWFVVALYNRLVGARNGYKNAFAQIDVQLTRR
                     YDLIPNLVETVKGYIKHERETLEAVIKARNTAMSGLKAAAANPGDAAAMTALSGAEGQ
                     LGGALGRLFALAEAYPDLKANQNMMQLSEELTSTENKVSFARQAYNDAVMGYNNQVEM
                     FPGSIIAGMFNFATAQLLELAKPEAREAPKVSFT"
     gene            7163..7585
                     /locus_tag="VEW08_11045"
     CDS             7163..7585
                     /locus_tag="VEW08_11045"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HYJ41319.1"
                     /translation="MFPRGITLFELLVVLVVVGILAAVALPAYRHHTLRVNRTEAITA
                     LLQLQSAEEAYYLRHDTYTASVEAAPPAGLGISAASTSGKYALSVALAADGQSYIATA
                     SPMPGGVQGADQECLAFSIDARGRRAVSGAAGVQRCWK"
     gene            7619..8581
                     /locus_tag="VEW08_11050"
     CDS             7619..8581
                     /locus_tag="VEW08_11050"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYJ41320.1"
                     /translation="MKNRAAVAASLLLSFTGPGQAAPAELKLATWNLEWFMTQETLRA
                     LTPACLPADAPRDGAKRAVPCDVAHELARSGEDIAALKRHARRLNADVIALQEVDGPE
                     AARLVFPDYQFCFSGRIAVQNNGFAIRRGLPFLCAPDLADLSMNDDVRRGVELRLFPN
                     TPQELRLLSVHLKSGCARDSLDSTRPGCGVLARQVPVLERWIDAQAADKKPFAVFGDF
                     NRDLRREPSGVSVWAEIDDASPPEADLVNTAEGQVFQNCMPSQTFSGYIDYIILGRQM
                     ARGLVKDSFGRELFPPKDALRRKLSDHCPVFIRLRVADAVRGAG"
     gene            8675..8983
                     /locus_tag="VEW08_11055"
     CDS             8675..8983
                     /locus_tag="VEW08_11055"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HYJ41321.1"
                     /translation="MSKTTTAGIQSVERRLHTRHRARTTVYITTPHGRKKLCRAINLS
                     ATGVFVETTNLGLRKGQQVELAFAINLGAVTKLHRRTAVVAHVSRGGTGLMMDAYTAR
                     "
     gene            complement(9092..10318)
                     /locus_tag="VEW08_11060"
     CDS             complement(9092..10318)
                     /locus_tag="VEW08_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ41322.1"
                     /translation="MLNEQVIEVAPQWVATLEQALPHARYRAIALYDARGEVLHCTST
                     DFGIEFRQALNDALDAFVLGAARESRFLRGEGTRTAVMLALRDLHSALQGICLLVVED
                     SPLEDEDPAARFLTPAVTTILADIASEVAQTLHAMPRAKPPKPKPPVPLPLEAVYAQI
                     RNQEVVLRVQQLVRLRKAEGIRRFEVLLRHMVDGEEQSPERVMQTAANHGLASMIDRR
                     VIGELIAWLHRNPTVWKKDPPTFSVNLSDAAVIEPHFISFVESCLQKAALPPGLIGVE
                     FSERVCLTHPDQVIPALDVFGRIGVPVAIDDFNVVGAGMPILDHSAVRLLKIDAELTT
                     NALQHRLTQARVVGLVQTAKVMGLQTVAKRVMGGDHSNWLTALGVDFVQSFEASPPVA
                     LDSLLGPVPRENLSQG"
     gene            10686..>12104
                     /locus_tag="VEW08_11065"
     CDS             10686..>12104
                     /locus_tag="VEW08_11065"
                     /inference="COORDINATES: protein motif:HMM:NF044450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HYJ41323.1"
                     /translation="MKKRISRRQFISTSAATTAVLGAAALEAGPLASSSPVISTPNPG
                     AGAWVRWLDSTAATVAQGVTWGTPWPRGKQRETKNFALRGTDQKLQSLQSWPLAYWPD
                     GSLKWTAHALPPPEVTGSGPFEVVSRPGAAKLPVSVSVKETDGAIEVDTGKFVCRLAR
                     TGSNLIDTISRGGHEALRAGKLVLLLQDRAASSGDAQVRQENFESAIEKVTVEQRGPV
                     RAVIKVEGRHSNGTRQWLPFTLRLYFYAGGESLRVLHTIVFDGDESKDFIRGIGLRFS
                     APLTDALHDRHVRFVGDQGLFAEAVRGLTGLRRDPGAAARKNQIDGIAAGAISPVVTE
                     RLQYIPAFGDWTLLQPNANSFTIRKRTADGHAWLDAGQGHRASGLGVVGGPSGSIAFG
                     IRNFWQSHPAQLDIRHAHENQAEVTMWVWAPDAIPMDLRFYHDGLGQDTYEKQLKGGL
                     EITYEDYEPGFGTPMGVARTSEL"
BASE COUNT         2240 a         3938 c         3768 g         2158 t
ORIGIN      
        1 cttctcttcg ggcgccttgt cgatctggtc gtacgccatg tacgagccac cgtgcgtctc
       61 cgccatcacc ttggtgagcg cagccgtcag cgtcgtcttg ccatggtcga cgtggccaat
      121 ggtgccgacg ttgacgtgcg gtttattgcg ttcgaacttt tcttttgaca cggagataac
      181 ctctgaaacc tattgcctaa actcaagact caagacaccc aagcggaact ggtgctcacg
      241 agcgggatcg aaccgctgac ctcgtcctta ccaaggacgt gctctaccaa ctgagctacg
      301 tgagcttcct ggagcgggtg gcgggaatcg aacccgcgtc atcagcttgg aaggctgagg
      361 ttctaccatt gaactacacc cgccgatgac gcttaaggct cacgcctcaa aaacattaac
      421 ccgcgaaaat ccaccgacta tctacccctc gaacttcgtc cttccttccc acccaaaatg
      481 aatggtggag ggggaaggat tcgaaccttc gaaggcgtaa gccggcagat ttacagtctg
      541 ctcccgttgg ccgcttgggt acccctcccg ggaaaaacga gccgcgtatt ctcgtttcga
      601 ccctccggag tgtcaacggc attggcgcgt attttgctaa ggaattaagt cccggcaccc
      661 atctccgcac cccgcccgtt cggcggcact catctatata ggggaaggtg ctggtatcaa
      721 ggcccagccg gggccccgct tgcgaaggat cgcagcctgc cgagctgaat tcccatgacc
      781 tggtccacca gcggcgccag tttggccgaa tccatcgcca ggactccggc gatccgataa
      841 gtcatgttga tccgggttcc gggtccatcc ggcgccaggt tgaaactgag gacgccgcta
      901 acggccatgt cctgcagtgg acccagtgcc ccgtccaacc tgagcaactt ccctggttgg
      961 gcaaagatca cccgtgcgtg ttgcaccgaa ccaccgtcgg cgagcttctc gcaaaagcag
     1021 cctccggcct tggcttcgag cttcaggttt cgggcggaac cggaccaggt gtgtttggga
     1081 tcccaccagt ccgccaccct gatcagctgc cggtagaccg ctgccggatt ggcatccgcc
     1141 atgacggttg cctcgatgac gaatccgttt tcccgggtct ctttcacgtc ggcatggacc
     1201 ggcgatgcga ggctaatggc caaggtcacg atcgggagga ggaagccgtt tttcatgggg
     1261 actccgatcc cgcgaacgcg agattcgata gttcggtaat tgcgggacgg ggtagcgctt
     1321 tgaacgcacc accgggtaaa tccttgggta attcaacgct accgtatctc aggcgggtca
     1381 atcgactgac gatgtagccc tcgtgttcga agaggcggcg aacctcgcgg ttacgtcctt
     1441 cgtgcaggca gacgcgaagc caggtgtggc tgccttcgac tgactgaacg tggatctcat
     1501 cgaaattcgc ctccccatcc tcgagctcaa cgccccgtcg cagacgctcg aggacctcgg
     1561 ccgggggcga gccacggagc cgcacgaggt actcacggcg aatctcgctc gaggggtgca
     1621 tgagccgatg cgccaggtcg ccgtcgttgg tgaacagcag cagccccgag gtattcacgt
     1681 ccagccggcc aacgacgatc cagcgaccgc tcgatggctc cggcagacgt tcgaaaacgg
     1741 tggggcggtt ttccggatcg gcgcgggtgg tcacctcgcc cacgggcttg tgatagagca
     1801 gcacctcgac ggactcgcga cgcccgccca gatcgagcgg cttgccatcg aggcagatcc
     1861 ggtcggcatc ggaagcgcgg tcgcccagca ccgcaacgcg atcgttgatg gtcacgcggc
     1921 cggcgcgaat ccactcttcg atgccgcggc gcgaaccatg cccggcaccg gccaaaagct
     1981 tttgaagtcg ctcagtcatg aggggcggca gcataaccaa ctgttatatg gctattccaa
     2041 gcccagcctc gtctcccctc agtgacccgc ctacagtccc cgcaccatgc tctacgacaa
     2101 catcctcggc acaatcggcc gcactcccat cgttcgcatc aaccggctcg caccggcggg
     2161 cacgacgatg tacgtgaagt gcgaattctt caatccgctg tcgtcggtca aggaccgcct
     2221 ggcgatcgcc atcatcgaag acgcggagcg cagcggcacg ctgaagcccg gccagacggt
     2281 ggtggaagcc acttcgggca acacgggcat cgcgctggcc atggtctgcg ccgccaaggg
     2341 ttaccccttc gtcgccgtga tggtggaaac cttctccatc gagcgccgca agatcatgcg
     2401 catgctcggc gccaaggtga tcctcacgcc tgccgccgaa cgcggccagg gcatggtgcg
     2461 caaggccgca gagctcgcgg agaagcacgg ctggttcctc gcgcgtcagt tcgaaaatcc
     2521 ggccaatccg gcttaccacc gcaacacgac ggggccggaa atcatccagg acttcgtcgg
     2581 caagcgtctc gacaatttcg tcaccggctg gggcacgggc ggaacgctca ccggcgtcgg
     2641 tgaggtgctc aagctcacgc gccccgacgt caaggtaatc gtcagcgagc ccgaagccgc
     2701 gcagttgtta ggtggcaagc cattcgcgcc gcacaagatc cagggctgga cgccggattt
     2761 cgtgccggcg gtgctcaatc ggaatgtcgc cgatcgcatc gtgcccgtga ccgacgtcga
     2821 agccatcgag tcggctcgcg cgttggcacg caccgaaggc atcttcgtcg gcatctcgag
     2881 cggcgccacg ttcgccgcgg cgctcaaggt ggcacgcgaa gccggcccgg gttcggtcac
     2941 gctggccatg ctgcccgaca cgggcgagcg ttatctatct accgcgctgt tcgagggaat
     3001 ctcagacggc ggcgatccag agccatgagt ggacaaccgc gcgaaaaatt cggtcatgcc
     3061 gactcgaaag agatgaatcg cgcggttgct gccgagcttg ccgaggtgag cttgggtccc
     3121 gccacaaagt tcgccgcact gcccgacccc ttccgtttct ggcgcggcgg cgcgctgtcg
     3181 gaaggcaggg tggcctacga gacctggggt tcgctgaatg cggcgcgcga caacgcggtg
     3241 ctgttgttca cggggctctc gccgtcggcg catgcggcgt cttcgccgga ggatccatct
     3301 tccggctggt gggaaaagat ggtcggcccc gacaaggcca tcgataccaa tcgatacttc
     3361 gtggtctgcg tcaattcgct gggcagctgc ttcggctcct cggggcctgc gtcaatcgat
     3421 ccggccactg gactcgccta ccgtctggat ttcccggacc tttccatcga agacatcgcg
     3481 cgcggcggct acgaaaccat ccgttcgctg ggcatcgaca ggttgtccgc cgtagtgggt
     3541 gcttcgctcg gcggcatggc ggtgctggcc tttgcggtgc aattcgccgg tgtggcgcgc
     3601 cgggtcatca gcatctccgg cagccccgcg gccatgccct tcgccatcgc actgcgctcc
     3661 actcagcgcg acgccatcct cacggatccg gattggcagg atggccagta cacaggcgac
     3721 gtgcgcccca tcacgggcat gcggttggca cgcaagctgg gcaccctcac ctaccgctca
     3781 gccgcggagt ggaagcaccg cttcggccgg cgtccggctt ccgatcgccg ctacgaaacg
     3841 ctgtcaccgg gtcgcgcgca ttttccggct gagttcgcgg tgcaggacta tctgaacgcg
     3901 caggccgaca aattcgtctc ggtgttcgat cccaactgct atctctacct gtcgcgcgcc
     3961 atcgatcgct tcgatctgtc ggcgcatggc ggcacgcccg tggccgcgct gaaacgcgcc
     4021 gcggcggaac gcgcgctaat cattggcgtg gagtccgaca ttctcttcac catcaacgag
     4081 cagaacgccc ttgccgaggc gttcaccaat gcgggcaccg ccacggattt cgtgccgctt
     4141 gcctcgctcg agggccacga cgccttcctc gtggatatcg agccgtttgc cgcggccatc
     4201 ggaaaattcc tgggcgaata atcgcgtgta acgcgaactt cgcgcgccgt taacgagcgt
     4261 gcaagaccgg ttcctcaagc tgcgcctgaa aaacaggaat cagcaaagga actgtcatga
     4321 aacattcaaa tctcgccgaa cgcctcgagg aattcgtcgc tgctctcagc gtcatcgatc
     4381 agccggagcc ggacacaaac acgactcgaa ttctcgccgg cgtagccagg cccgcgaaac
     4441 aggcaccgcg gcgcctctcc ctgactgcaa ggcacatcca gatccgcgac cagggcctgc
     4501 gcccgttccg cgtgtcctaa ctacctggcg cgggcgccga gaggatcggc ggcagcgggc
     4561 aatgcagcac cgaacagacg gtgcgcagca gctcgccttc ttccaccgag agcttctggt
     4621 cgtgcgcgat gcagcgcacg agcccctcga tgagcacttt cttggcgaat ggccgaaggt
     4681 tggccaggcg cgccagcgaa tcggccagcg ctttcggcca tcgtgcgatc tgctcgtagg
     4741 ctggccgatg ttcgggcagc accaccgaga tacccgcctc gtaggcgcgg cgtgcgcgcg
     4801 catcgtcgcc cgtgccttgc gcggccagga tggcgaacaa ggcgtggatc gccgggatct
     4861 cctgcatgag cgactcgctg ccgtgcatcg ggccggcctc gagatcatcg aggttcgatg
     4921 ccagcagcaa ggtgaggcag cactcgaaca cgtcgatgcg cgcatcggcg ttcgctagct
     4981 gcgccacgac gtcgcgcagc cgctgccgtt cggtcagcga caggcgccgc agggccggaa
     5041 aaagctgctg caccgctggc aggcgcagcg ccggcgccag tgagtcggcg agtgtctgca
     5101 tgccaaagac ccgtgcggtg aactcaggcc cgtacacggc ttcgaggatg cgccgctgct
     5161 cggcctgaac ttcgggaatc ctgctggcca gcaccaccag catcagcgcg cgcgcgtgat
     5221 cggcggagtc gacgaaatcg cgcagcgctt cgggaatggc aacacgcgcg ttctcggcgt
     5281 accgcacatt ttcattggcg atggtgccgg ccagcgccgc gatgtcgtgc gccgcgtggc
     5341 cgcgaatgct gcggtcgccc gccggcgtga atgcactcgc gctcgactca tgacgctgcg
     5401 actgaggtgt gcgggtggca tcttcggcgc gttgccgctc cgagtcgcgc gcggcatcgg
     5461 ccgcgagctt cggcaactcg ctggcgcgaa atgacggatc cagtgccttc agccgctctt
     5521 cgagcggagg atgagtggag aacaaacgtg gaagtcccgc ggcgaacaac atgtgcgcca
     5581 cttcttcggc ttccggcgtg accaggcgcg agccggcacc cgcgcccgct atcttcagca
     5641 gcgcgccggt caagcccacc gggttgcgcg tgaactggac ggccgatgca tcggcaaggc
     5701 gctcccggtg ccgggagacc gccgcctgca acaggcgccc gaagaacacg ccgatgtagc
     5761 ccagcaccat cgccgccagg gccgcgatca tgagcgcgcc cgcaccatcc ttgcggctcc
     5821 ggccgccaga gccgggcgag ctctgcagca cgacgcgcgc cacgatagcc accgccagga
     5881 gcccgaaagt ccagcctaac aagcgaacgt tgaggcgcat gtcgccgttg aggatgtggc
     5941 tgaattcgtg cgcgatcacg ccctgcaatt catcacgctt gaggcgcgtg gcggcgccac
     6001 gcgtcacggc gatggcggca tcggcgggtg aattgcccgc cgcaaaggca ttgataccgt
     6061 cttcgtgctc gagcacgtac acctcgggca tggtcacacc cgaggcgatg gacatctctt
     6121 cgacgacgtt gtgcacgcgc ttcaacgcaa tgtcgcctgt gcctcgctcg attcgcgtgc
     6181 cgccgagcga acgcgcgacc acaccgccgc cggcgcgcaa cgagagggtc ttgaacaagc
     6241 tggcgccgca gatgatgccc agcaccacga tgctgctgcc gaccaccgcg ttcaccggcg
     6301 atgtaccggg atcagaagaa gcgatgacga aggcgaccac cgcatcgacc gcggcgacca
     6361 ccaggactac agaaatcagg aacgcaacca ggagccagcg cgtcgtgcga cgcgcggctt
     6421 cctggcgggc gtagaaatcc attagttagg tgaagctgac cttcggcgcc tcacgcgcct
     6481 cgggcttggc gagctcgagc agctgcgcgg tcgcgaaatt gaacattccg gcgatgatgc
     6541 tgcccgggaa catctcgacc tggttgttgt agcccatgac ggcgtcgttg tacgcctggc
     6601 gggcgaacga caccttgttc tccgtcgagg tgagctcttc ggagagctgc atcatgttct
     6661 ggttggcctt gaggtcggga taggcctcgg cgagggcgaa taacctgccg agagcgccac
     6721 ctagctggcc ttcagcgccc gaaagcgcgg tcatggcggc ggcatcaccc ggattggcgg
     6781 cggcggcctt gagtccgctc atcgccgtat tgcgcgcctt gatgacggcc tcgagcgttt
     6841 cgcgttcgtg cttgatgtaa cccttgacgg tctccacgag gttcgggatg aggtcgtaac
     6901 ggcgtgtgag ctgaacgtcg atttgcgcga aagcattctt gtacccgtta cgcgcgccga
     6961 ccaggcggtt gtaaagcgcg acgacaaacc agatcgcgac gacgactagc gcaagcaaaa
     7021 tccaacccat gatgaaactc cctgatatgc gcccgtggcg cgctcgaaat atgggtgcgt
     7081 cgacccgtca ttgcaagtat ctgggccatg tagtgagttt tgcgcctcac gccgaagggc
     7141 ggtctcgtgt tgacgtgccg gcatgttccc gcgcggcatt accttgtttg aactgctcgt
     7201 cgtgctggtg gttgtcggca tcctcgctgc tgttgccctg ccggcctatc gccaccacac
     7261 gctgcgcgtg aatcgcaccg aggccattac cgcgctgctg caattgcaaa gcgccgaaga
     7321 ggcctactac cttcggcacg acacctacac cgccagcgtc gaggcagcac cgccggctgg
     7381 cctcggcatt tccgccgcga gcacgtcagg caagtacgca ctttcggtgg cgctcgcggc
     7441 cgatggccag tcatacatcg caaccgcctc tcccatgccc ggtggcgtac agggcgcaga
     7501 tcaggagtgc ctcgcctttt ccatcgatgc tcgcgggagg cgcgcagtaa gcggagcggc
     7561 aggcgtccag cgatgctgga agtaacaatg gtgtgttccg catgcgatag tcgcgcgcat
     7621 gaaaaatcgc gccgcggtcg ccgcctcact gctgttgtcg ttcacgggtc cgggccaggc
     7681 cgcgccggcc gagctcaaac tcgccacgtg gaatctcgaa tggttcatga ctcaggaaac
     7741 gctgcgggcg ttgacgccgg cgtgcttgcc agcggatgcg ccacgcgatg gcgcgaagcg
     7801 ggccgtgccc tgcgacgttg cccacgagct cgcgagaagt ggcgaagaca ttgcggcgct
     7861 gaagcggcac gcacgcaggc tgaatgcaga cgtcatcgca ttgcaggaag tggacggccc
     7921 cgaggccgcg cggctggtgt tccccgacta ccagttctgt ttctcaggac gcatcgcggt
     7981 ccagaacaat ggcttcgcga tccgtcgcgg cctgccgttc ctctgcgcac ccgaccttgc
     8041 cgatctgtcg atgaatgacg acgtgcgccg tggtgtcgaa ctgcgcctgt tccctaacac
     8101 accgcaagag ctgcggttgc tctcggtaca cctgaagtcg ggttgtgcac gggattcgct
     8161 ggactccacg cggccaggtt gcggcgtatt ggcgcgccag gtgccggtgc tcgagcgctg
     8221 gatcgacgcg caggcggccg ataaaaagcc cttcgccgtg ttcggggact tcaaccggga
     8281 cctgcggcgc gaaccttccg gggtctcggt ctgggccgaa atcgatgacg ccagcccgcc
     8341 cgaagcagat cttgtcaaca cggccgaggg gcaggttttt caaaactgca tgccttcgca
     8401 gactttcagt ggctacatcg actacatcat cctgggccgc cagatggcgc ggggattggt
     8461 gaaagactcc ttcggacgtg agcttttccc gccaaaagac gcgctgcgcc gcaagctgtc
     8521 ggatcactgc ccggtattca tccggcttcg ggtggcagac gccgtccgcg gcgcggggta
     8581 aatcgaaggg cgtgaatttt ttgccgaaac ctattaagtt cagttcggtt caaagaagca
     8641 tctcgcaagg caataatccc tgtaggagag cctgatgagc aaaacaacga cggcggggat
     8701 tcagtctgta gagcggcggc tgcacacgag gcaccgcgcg cgcactacgg tctacatcac
     8761 cacgccccat ggccgcaaaa aactctgccg cgccatcaac ctctcggcca ccggtgtgtt
     8821 cgtcgagacc accaacctgg ggttgcgcaa aggtcagcag gtggaactcg cgttcgccat
     8881 caacctcggc gccgtcacca agctgcatcg ccgcaccgcg gtagttgcgc acgtgtcgcg
     8941 cggcggcacc ggcctcatga tggacgccta caccgccagg tagttagaac gtctttgtct
     9001 ttcagtttga tggacgccgg cgcttcgccg gcgtttttcg ttgtggggcg tgagcccctg
     9061 gaaatggagc ccctggaaat cccctgaaaa ttcagccctg agaaagattt tccctgggca
     9121 ctggccctaa cagactgtca agtgcaacgg gcggtgacgc ctcgaagctc tgcacgaagt
     9181 ccacgccgag cgcggtcaac cagttgctgt gatcaccacc catcacgcgc ttcgcaacgg
     9241 tctgcagccc catcactttc gccgtctgca ccaggccgac aacacgcgcc tgcgtgaggc
     9301 ggtgctgcaa ggcgttggtg gtgagctcgg cgtcgatctt gagaaggcgc acggccgagt
     9361 gatccaggat gggcatgccc gcgccgacca cgttgaaatc gtcgatggcg accgggacac
     9421 cgattcgccc gaatacatcg agcgccggga tgacctggtc cggatgcgtg aggcacacac
     9481 gttcggaaaa ctccacgccg atgaggcccg gcggcaaggc cgccttttgc aggcagcttt
     9541 ccacgaagct gatgaaatgc ggctcgatca ccgccgcatc ggaaaggttc acggaaaacg
     9601 tcggcggatc ctttttccag acggttgggt ttcggtgcag ccacgcgatg agctcgccga
     9661 tcacacggcg atcgatcatc gacgccaggc cgtgattggc ggcggtctgc atgacacgtt
     9721 cgggcgactg ttcctcgccg tcgaccatgt ggcgcagcag gacttcgaaa cggcgaatgc
     9781 cttcggcttt gcgcaggcgc acgagctgct gcacgcgcag caccacttcc tgattgcgaa
     9841 tctgggcgta taccgcctcg agcggcagcg gcacgggcgg cttcggcttg ggcggcttgg
     9901 cacgcggcat cgcgtgcagg gtctgcgcaa cttccgaggc gatgtcggca agaatcgtcg
     9961 tcaccgccgg cgtgaggaat cgagccgccg gatcttcatc ttccagcggc gaatcttcga
    10021 cgaccagcag gcagattccc tgcaatgcgg aatgcagatc gcgcaatgcc agcatgaccg
    10081 cggtgcgtgt gccctcgccg cgcagaaaac gcgactcgcg ggccgcgccg agcacgaagg
    10141 cgtccagcgc atcgttcagc gcctggcgga attcaatgcc gaaatcggtg gaggtgcagt
    10201 gcaggacttc gccgcgagcg tcatacaacg cgatcgcgcg ataacgtgcg tgcggcagcg
    10261 cctgctcgag cgtggccacc cattgcggcg caacttcgat gacctgctcg ttgagcacag
    10321 acgcacgaat agtggtctgg cccgtatcag acgatgcaga ggccaatgtg gactgacgtg
    10381 gatccatctt ccctggattt ctcgatgtgc gttcccgaag cgagggttac agaatccggc
    10441 caatctgaac gtgatcggac tcgcggtcct gccgcaagtg ttaaaacggc ggcctgtcat
    10501 ttgacatatc ccagcggccg gctactccta tctaactagt gtcatcggcc cggaaaatgc
    10561 cccaccgcct tggcagcccc cataccagcc cctactttgg gtgctttgca gatccgatca
    10621 acatcgtgca tattcgatca cgaagaacaa ttcgcgcggg caggacgccc atcccggggg
    10681 attacatgaa gaagcgcatt tcgcggcggc agttcatctc aacgagcgcc gcgactaccg
    10741 ccgttctcgg agccgcggct cttgaagccg gcccgctagc gagctcgtca ccggtcattt
    10801 cgacgccaaa ccccggagcc ggtgcctggg tgcgctggct cgatagcact gccgccaccg
    10861 tggcacaggg cgtcacctgg ggcacgcctt ggccgcgcgg caaacagcgg gaaaccaaaa
    10921 acttcgcact gcgcggcact gaccagaagc tccagagcct gcagagctgg ccgctcgcgt
    10981 actggcccga cggttcgctc aagtggaccg cacatgcgct gccgccgcct gaagtgaccg
    11041 gcagtgggcc gttcgaagtg gtttcccgac ccggtgccgc gaaactcccg gtcagcgtga
    11101 gcgtgaagga aaccgacggc gccatcgaag tcgacaccgg caagttcgtg tgccgcctgg
    11161 cgcgcaccgg ctcgaacctc atcgacacca tttcgcgcgg cggacatgag gcgttgcgcg
    11221 cgggcaaact ggttttgctg ctccaggatc gcgctgcgtc gtccggcgac gcgcaggtgc
    11281 gccaggaaaa tttcgagagc gcaatcgaga aggtgaccgt cgagcagcgc ggccccgtgc
    11341 gcgccgtcat caaggtcgaa ggcaggcact cgaatggcac gcgccaatgg ctgccattca
    11401 cgctgcggct gtatttctat gcgggcggcg aaagcctgcg tgtgctccac accatcgtgt
    11461 tcgatggcga tgagtcgaaa gacttcattc gcggcattgg tctgcgcttc tccgcgccgc
    11521 tcaccgatgc gttgcacgat cgtcacgtgc gcttcgtcgg cgaccagggc ctgtttgccg
    11581 aagccgtgcg cggcctcacc ggcctgcgcc gcgatcccgg cgcagcggca cgaaaaaacc
    11641 agatcgacgg aatcgcggcg ggcgccatca gccctgtcgt caccgaacgc ctgcagtaca
    11701 tccccgcctt cggcgactgg actctgctgc agccgaacgc caactcattc acgatccgca
    11761 agcgcactgc cgacggccac gcctggctcg atgcaggcca aggacatcgt gcgagcggcc
    11821 ttggagttgt cggtggtccg tccggcagca tcgcctttgg catccgcaat ttctggcaga
    11881 gccatcccgc acagctcgac atacgccacg cgcatgagaa ccaggccgag gtgacgatgt
    11941 gggtgtgggc gcccgacgcc atcccgatgg acctgcgctt ctatcacgac ggcctcggcc
    12001 aggacacgta cgaaaaacag ctcaagggcg gcctcgagat cacttacgaa gactacgagc
    12061 ccggcttcgg cacacccatg ggcgtcgcgc gcaccagtga gctg
//
LOCUS       DASQJS010000275         3750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_794724, whole genome shotgun sequence.
ACCESSION   DASQJS010000275 DASQJS010000000
VERSION     DASQJS010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794724"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /gene="gspL"
                     /locus_tag="VEW08_11070"
     CDS             complement(<1..580)
                     /gene="gspL"
                     /locus_tag="VEW08_11070"
                     /inference="COORDINATES: protein motif:HMM:NF016989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HYJ41324.1"
                     /translation="MSESLVIQLREGESPQWMVCNGDGQVVVEAVSGELVQATAMSTG
                     RRVAVILPAGEALATESDAPAKGAAKLAQVVPYALEERVADEIEHLHFAIGVRDPATG
                     RVPVVVIERARLESRLAELRAAGLNPQAIYSAATLLPAMPGQMIGLLEGDTLTLRTAD
                     APPLVLPALSISDAFEMALSMQTSAVAGLEPAP"
     gene            complement(585..1547)
                     /gene="gspK"
                     /locus_tag="VEW08_11075"
     CDS             complement(585..1547)
                     /gene="gspK"
                     /locus_tag="VEW08_11075"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HYJ41325.1"
                     /translation="MNHARQRGVALLVAIVMFAIATTVAAAITYNKAMAARRAAASFT
                     LEQALQAGMAAEALAAIALEDDGKNSKTSPDNAWAEPFGPVEIEGTGIWIQAQIEDLT
                     SRFNLNTLVRWQGPPDNLFVANPEQVQVFRKLLETLKIDANFADLLVDWIDTDIAPQA
                     TQGGEDTLYLTQAPPYRPPNTFIYHPSEILALPGFGPERYALLAPYITALPINAKINP
                     CTASGLVLDVTMNDPQNEQEFQLQDMPALRKKGCYPIGSVYTGRIGDPNLKTLADARI
                     EEKSSWFRLRTNIRIGTAEFVLYSVLFRQQNNRVRTVQRSFGSE"
     gene            complement(1544..2170)
                     /gene="gspJ"
                     /locus_tag="VEW08_11080"
     CDS             complement(1544..2170)
                     /gene="gspJ"
                     /locus_tag="VEW08_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HYJ41326.1"
                     /translation="MRTRGFTLIEVVIAMFIAAIMFAIGYGAINQALRDRDALNVSQG
                     RVTEIQLGMRVVAQDFAQVVARPARDTSGTGQLMPAMFASGNSDVIVTFTRAGWSNPA
                     GIQRPAEQRVRYRFFDGSLVREHWLAVDPALNTEPRQRTLLTKVKAVEIRFLDPVSRQ
                     WRTDWQANASGVVAPNAVSLLVTRPIAIEFTVVFEDWGRVIRLFEIPT"
     gene            complement(2170..2748)
                     /gene="gspI"
                     /locus_tag="VEW08_11085"
     CDS             complement(2170..2748)
                     /gene="gspI"
                     /locus_tag="VEW08_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HYJ41327.1"
                     /translation="MKNSPRQRGFTLLEVLIALAIVAMSVGALLGTITSSASNIIYLK
                     EKTLAEWVALNRLAEVRIDQQMPAKGKRSGNTVMAGMRWEWQEEVIELPIKGMFRVEV
                     RAHATGETVDDTRAVEKPAAQTLSPSTSSGSSKDSIAWTTSAVGVVGSARSDVREAVA
                     APFEGPPQNQQTGGPAGAPGAPAPPKVGAPSS"
     gene            complement(2745..3323)
                     /gene="gspH"
                     /locus_tag="VEW08_11090"
     CDS             complement(2745..3323)
                     /gene="gspH"
                     /locus_tag="VEW08_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HYJ41328.1"
                     /translation="MRATRAQSRGFTLLELMVVVFIIGLITAAAVITFGGDRRDTELD
                     KEAERIDALLGYVREQAELQTRDYGIRINDHTYSFVVFDVMQNEWRTNDEDDALRERE
                     FPEGLVPSVVLEGRPIVLNVQKDDKKGLEDFKPQIMVFANGDLSSFEITLQREGGGEK
                     ARIYTDDQADILMMLPGDKEPRGKRARTVQRR"
     gene            complement(3328..>3750)
                     /gene="gspG"
                     /locus_tag="VEW08_11095"
     CDS             complement(3328..>3750)
                     /gene="gspG"
                     /locus_tag="VEW08_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HYJ41329.1"
                     /translation="AARGFTLIEIMVVVVIIGLLAAVILPNVFGNVERAQVSKAKSVI
                     QAIETALTMYKLDNYKYPNTDLGLTALSQRPNDPTVRNWREGGYIKRISNDPWGNPYQ
                     YVFPGTRGQEYDLYSFGADSQEGGEGPNADIGNWNLGQ"
BASE COUNT          653 a         1150 c         1207 g          740 t
ORIGIN      
        1 gcggggcggg ttcgagtccg gcgaccgccg acgtctgcat cgacagcgcc atctcgaagg
       61 cgtcgctgat ggagagggcc ggcagcacga gcggcggagc atcggccgtg cgcagcgtca
      121 gcgtgtcacc ctcgagcaag ccgatcatct ggccgggcat ggcgggcaac aatgtagcgg
      181 cggagtagat agcctgggga ttcagacccg cggcgcgcag ctcggccagc cgcgattcga
      241 ggcgcgcacg ctcgatcacc accaccggaa cgcggcccgt ggccgggtcg cggacgccga
      301 tcgcgaagtg caggtgttcg atttcatccg cgacgcgttc ttcgagtgcg tacggaacca
      361 cctgggcaag tttcgctgcg cccttggcgg gtgcatcact ttcggtggcg agcgcttcgc
      421 ccgccggcag gatgaccgca acccggcgcc cggtgctcat ggcggtggcc tggactagct
      481 cgcccgacac ggcttcgacc accacctggc catcgccgtt gcacaccatc cactggggtg
      541 actcgccctc gcgaagttga atgaccagtg attcgctcat ctgatcactc gctgccaaag
      601 ctgcgttgca cggtacgtac tcgattgttt tgctggcgga atagaacact gtacaaaacg
      661 aactccgctg tgccaatgcg aatgttggtg cggagcctga accaggaaga tttctcctcg
      721 atgcgggcat ccgccaatgt cttgaggttg ggatcgccga ttcggccggt atagaccgag
      781 ccgatgggat agcaaccttt cttgcgcagg gccggcatgt cctggagctg gaattcctgt
      841 tcgttctgcg ggtcgttcat ggtgacgtcg agcaccaggc cgctggcggt acagggattg
      901 atcttcgcgt tgatgggcaa ggctgttatg taaggggcta gcaacgcgta gcgctcgggg
      961 ccgaaacctg gcaacgccag aatctccgac ggatggtaga tgaaggtatt gggtggacgg
     1021 taaggcggcg cctgggtgag atacaaagtg tcttcgccac cctgcgtcgc ctgcggcgcg
     1081 atgtcggtgt cgatccagtc caccagcagg tcggcgaagt tcgcgtctat cttcaatgtc
     1141 tccagcagct tgcgaaacac ctgcacctgt tccgggttcg cgacgaacag gttgtccggc
     1201 ggcccctgcc acctgaccag cgtattgaga ttgaaacgcg aggtcagatc ctcgatctgc
     1261 gcctggatcc agatgcccgt gccttcgatt tccacgggac cgaagggctc cgcccaggca
     1321 ttgtcaggag aggtcttgct gttcttgccg tcatcttcga gcgcaatggc cgcgagtgcc
     1381 tcggcggcca tgcccgcctg cagcgcctgt tcgagcgtga agctggccgc ggcgcgacgc
     1441 gcagccatgg ccttgttgta ggtgatggcg gcggccaccg tcgtcgcgat ggcgaacatc
     1501 acgatggcga cgagcagtgc aacaccgcgt tgacgtgcgt gattcacgtc ggaatctcga
     1561 acaggcgaat gacgcggccc cagtcctcga acaccaccgt gaattcaatg gcgatcgggc
     1621 gggtcaccag caggctgacc gcgttcggcg caaccacgcc actcgcgttc gcctgccagt
     1681 cggtgcgcca ctggcgcgag acgggatcga ggaagcgtat ttcgaccgcc ttgaccttgg
     1741 tgagcagcgt gcgctgtcgc ggctcggtat tgagcgcggg atcgaccgcc agccagtgtt
     1801 cgcgcaccag cgatccgtcg aagaatcgat aacgcacgcg ttgctcggcc gggcgttgaa
     1861 tgcccgcggg attgctccat ccggcgcgcg taaaggtgac gatgacatcg ctgtttccgc
     1921 tggcgaacat cgccggcatc agctggcccg tacccgaggt gtcgcgggcc gggcgcgcca
     1981 ccacctgcgc gaaatcctgc gcaaccacgc gcatgccgag ctggatctcc gtgacccgac
     2041 cctgcgacac attgagcgca tcacgatcgc gcagcgcctg attgatggcg ccgtatccga
     2101 tggcaaacat gatcgccgcg atgaacatcg cgatgaccac ttcgatgagc gtgaagccgc
     2161 gtgtgcgcat cagcttgatg gtgctccgac cttcggcggt gcaggagcac ctggagcgcc
     2221 agccggaccg ccggtctgct gattctgtgg agggccttcg aacggcgccg ccaccgcctc
     2281 gcgcacgtcc gaacgcgcgc tgccgaccac gccgacagcg gatgtggtcc acgcaatcga
     2341 gtctttcgaa gaaccgctcg acgtgctggg tgaaagggtc tgcgccgccg gcttttcgac
     2401 ggcccttgtg tcatcgaccg tttcgccggt ggcgtgcgca cgcacctcga cacggaacat
     2461 gcccttgatg ggaagctcga tgacttcctc ctgccactcc cagcgcatgc ccgccatcac
     2521 cgtgttgccg ctgcgcttgc ccttggcggg catctgctga tcgatgcgga cttcggcgag
     2581 gcgattgagc gccacccatt cggccagggt cttttccttg aggtagatga tgttgcttgc
     2641 cgacgaagtg atcgtgccga gcagcgcgcc gaccgacatc gcgacgatgg cgagcgcgat
     2701 cagcacctcg agcagcgtga aaccccgttg gcgcggcgag tttttcatcg gcgttgcacc
     2761 gttcgcgcgc gtttcccccg aggctccttg tctcccggaa gcatcatgag gatgtcggcc
     2821 tggtcgtcgg tgtagatgcg tgccttttca ccaccacctt cgcgctgcag ggtgatttcg
     2881 aacgacgaca gatcgccgtt ggcgaacacc atgatctgcg gcttgaagtc ttcgagtcct
     2941 ttcttgtcgt ccttctggac gttgaggacg atgggtcggc cttcgagcac gaccgaaggg
     3001 accaggccct cgggaaattc acgttcgcga agggcatcgt cttcgtcgtt ggtccgccat
     3061 tcattctgca tcacgtcgaa caccacgaaa gagtaggtgt ggtcgttgat acgaatgccg
     3121 tagtcgcggg tctggagttc ggcctgctcg cgcacgtaac cgagcagggc gtcgatgcgt
     3181 tcggcttcct tgtcgagctc ggtgtcacga cggtctccgc cgaaagtgat gaccgccgcg
     3241 gcggtgatga gaccgatgat gaataccacc accatcagct cgaggagcgt gaagccgcga
     3301 gactgggcgc gcgtggcgcg catggaatta ctgcccgaga ttccagttgc caatgtcggc
     3361 attcggaccc tcgccgcctt cctggctgtc ggctccgaaa gaatagaggt cgtattcctg
     3421 tccacgtgtg ccagggaaca cgtactggta aggattgccc cacggatcgt tgctgatgcg
     3481 cttgatgtaa ccgccttcgc gccagttgcg caccgttggg tcgttcggac gctgcgacaa
     3541 cgccgtgaga ccgaggtcgg tgttcgggta cttgtagttg tcgagtttgt acatcgtgag
     3601 cgcagtttcg atggcctgga tgacgctctt ggctttggaa acctgtgctc gctcgacgtt
     3661 tccgaagaca ttgggcagga tcacggccgc cagcaggccg atgatgacca cgaccaccat
     3721 gatttcgatg agggtgaagc cgcgtgcggc
//
LOCUS       DASQJS010000276         7513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1735803, whole genome shotgun sequence.
ACCESSION   DASQJS010000276 DASQJS010000000
VERSION     DASQJS010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735803"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="VEW08_11100"
     CDS             complement(<1..1442)
                     /locus_tag="VEW08_11100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HYJ41330.1"
                     /translation="MSRTTEAVNRIPAQVSIGALALLCGCFVFSGVAALIYQTAWTRQ
                     FAIVFGTSELAVATVLAAYMGGLALGATLAEKFLARVTRPVLTYGLLEIGIAGSAVFI
                     VPMLLWLANVALQSLFGGQPTPPDSDHAGTTLFYLVSAFVALALPTTLMGATLPVLAR
                     YAVAEEAQIGRRIGLLYAMNTAGAVAGALLTAFLLLPELGLTRTVWIAAVLNAIVFLL
                     AVALARRMPHEKTPKGFADDATPRRAAPERIRKYSFKAFPGPAWVLPLMLFAGAVAFF
                     QEVLWTRMLAHVVGSSIYAFGVMVASFLAGIALGGALGAAVAKTRERATLALGLALMV
                     AAVAAAVAYLRLESLLPSTAGLLVSSDLHFGFLRVPNTLFCGLLLLPMTIAIGMTYPL
                     AVRILASDAQEAAPVSARVYAWNTVGAIAGSLAAGFLLIPELKYEGSIRVAVCASAAL
                     GVFALWVLLPVKRVVAIATTLLAVAGCVLF"
     gene            1474..2307
                     /locus_tag="VEW08_11105"
     CDS             1474..2307
                     /locus_tag="VEW08_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HYJ41331.1"
                     /translation="MAAFRANGIVTLTTDFGAREPFVGIMKGVMLGHAPHLRFIDMAH
                     EVSAFQPVEAGFWLAHALRYFPAGTLHVAVVDPGVGTPRALIIAFAGEQAVLAPDNGL
                     LAPLAARGTIDRVIQVDHARLVKFGVSDVSATFHGRDVFAPLAAAIAAGRCNPSDLGT
                     ETTTFSLEGWPATTPRKDGGVAGVIVAVDRFGNLISNIESSAVVALTMPIVHIGGLAL
                     PLKRTYGDARPGEYLGVINSFEVLEVAQSRGNAAKGLNLAVGASISAVPGVPDPQFRR
                     G"
     gene            2402..2626
                     /locus_tag="VEW08_11110"
     CDS             2402..2626
                     /locus_tag="VEW08_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41332.1"
                     /translation="MSERDNESFDLDEEFLAEAEDNTDYDRILDKVDRRVRQQPGTKK
                     VGKAAWSRLEEVLADRRLEKDLNDGFEDEQ"
     gene            2682..5267
                     /locus_tag="VEW08_11115"
     CDS             2682..5267
                     /locus_tag="VEW08_11115"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF006515.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HYJ41333.1"
                     /translation="MSARSASRWVVVKFGGTSVSTLINWRNIALVVKRRLDSGARVLV
                     VHSAITKITDMLEKLLAAAISGNPEEPLKAIEQRHRDLATELGVGMSAQLQGYFDELR
                     QMANGISLVRELSDKTRARVMANGELMATEIGSRFLKSQGVDVTWMDARDMLSAEDRG
                     ASAKASVLSATCSFAPDAGLEKRLDAATPVVITQGFIARDSEGNTCLLGRGGSDTSAA
                     YLGAKIRAKQIEIWTDVPGMFSANPRSTPTARLIRALHYDEAQEIATSGAKVLHPRCL
                     LPARQYQIPLYVYATQTPDLEGTLITGESGATAQVKAVCNRKGITLISLDSPGMWHQV
                     GFMADAFAVFKEHGMSVDLVSTSETNVTVSLDPAANTLDGELLGKLQRDLSKLCGVQI
                     IGPCASVSLVGRNIRAILHKLGDAFALFEEQKVYMVSQAANDLNFTFVVDENQGDRLV
                     EQLHDLLIHPLPGDSGFGPTWEQLFAKPEAVAAGVQPWWALKREALLGKLANRDSAYV
                     YDLDQVETAARAIAGIKSIGRALFAMKANPHPDIVRKVAAAGLGFDCVSINEIQHVLA
                     SVPGISPERILFTPNFAPRAEYERALELGVHVTIDNLFVLQHWLDAFKGKSVFLRIDT
                     GIGRGHHHHVKTAGAESKFGIPSEELDEVARLAKAGDVTVNGLHAHAGSGVFDVENWT
                     QVGSVLVGLVKRFPDVKFIDVGGGLGVPDRAQGRELDLARLDAALAKVAAAIPGVTLW
                     IEPGRYLVANAGVLLARVTQTKIKSGIRYVGVATGMNSLIRPALYGAHHDIVNLTRYG
                     EPANISANVVGPICESSDFLGHDRLLPECQSGDVLLIATAGAYGHAMSSSYNLRAPAE
                     ELLLK"
     gene            complement(5268..6215)
                     /locus_tag="VEW08_11120"
     CDS             complement(5268..6215)
                     /locus_tag="VEW08_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41334.1"
                     /translation="MRKSSVLLSVVAVASAAVSVSLWMELRAERARNAELSARLDAAG
                     EVRTVSVSEAPQVRKPAITPLPAVDPESDGAPGYSSQLTQEEEDLEAAERRHLRDPKY
                     REAWREERRLARAWLREDLIKLVGFTPQQADKVIDLSIDGQLRGLERTDSSLAEDEAA
                     EVAHQAKLREVLGADGVARLQAYYDSRPTRNQVNQLRNELTGVNVLRDDQVEPLIAAL
                     QAERAQMERELAAISDAADPNDISDATARKYAEIYAEQLQATHGRMHSSAAAILSRSQ
                     LETLDTMLRRERERNDAVQRMSRIQSTLDTPGDAATPPD"
     gene            complement(6230..6859)
                     /gene="hisIE"
                     /locus_tag="VEW08_11125"
     CDS             complement(6230..6859)
                     /gene="hisIE"
                     /locus_tag="VEW08_11125"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HYJ41335.1"
                     /translation="MSTALDIARVDFVKGEGLVPAIVQDADTGAVLMLAYMNREALEQ
                     TIARKRAVFFSRSKRRLWEKGETTGHALDVIDIALDCDQDTLLVTARPRGPACHNGTL
                     TCFGNESRAAVTPIAFLAKLEGIIAQRAAEKPEASYTAKLLDKGINRVAQKVGEEGVE
                     LALAGAAQGDDKVIEESADLLFHMLVLLRARGVSLSQVVSQLESRHTPR"
     gene            complement(6856..>7513)
                     /locus_tag="VEW08_11130"
     CDS             complement(6856..>7513)
                     /locus_tag="VEW08_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014998871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HYJ41336.1"
                     /translation="RYRNEGADEVVFYDITASPEGRSVDRKWVSRVAGILDIPFCVAG
                     GIRSVEDAEAVLNAGAEKISINSPAIANPGLIEELASRFGSQCVVIGIDSASLAEGYR
                     VFQFTGDPNRSRNTGRETLSWVKEVQERGAGEIVLNCMTNDGVRRGYDVAQLKAVREV
                     CDVPLVASGGAGAPQHFIDVFEQASVDAALAASVFHSGAIAIPDLKTTLAASGIEMRI
                     "
BASE COUNT         1352 a         2380 c         2438 g         1343 t
ORIGIN      
        1 ttgaaaagca cgcatccggc aaccgccagc aaggtggtgg cgatcgcgac tacgcgcttc
       61 acgggcagca ggacccataa ggcgaacacg ccgagcgctg cgctcgcgca cacggcgacg
      121 cgaatggatc cttcgtactt caattccgga atcagcagga atcccgcggc cagcgacccg
      181 gcgatggcgc ccacggtgtt ccaggcatag acacgggcac tgaccggcgc ggcttcttgc
      241 gcgtcactgg ccaggatgcg caccgccagt ggataggtca tgccgatggc aatggtcatg
      301 ggcaacaaca ggaggccgca gaacagcgtg ttggggacgc gcaggaagcc aaagtgaagg
      361 tccgatgaca ccagcaggcc ggcggtggaa ggcagcaggc tttcgagacg tagataggcc
      421 accgcggcgg cgaccgcggc caccatcagc gccagcccca gtgccagcgt tgcgcgttcg
      481 cgcgtcttcg ccactgccgc acccagtgcg ccaccgagag cgatgccggc aaggaagctg
      541 gcgaccatca ccccgaacgc atagatgctg ctgccgacga cgtgcgcgag catgcgtgtc
      601 cacaacactt cctgaaaaaa cgcgaccgca cccgcgaaca gcatcagcgg caatacccat
      661 gccggtccgg gaaacgcctt gaaggaatat ttgcggatgc gctccggcgc cgctcgacgc
      721 ggcgtcgcgt cgtctgcaaa tcccttgggc gtcttctcgt gaggcatgcg gcgagccagc
      781 gcgactgcca gcaggaacac gattgcgttg agcactgctg cgatccacac cgtgcgcgtg
      841 agcccgagct ccggcagcag caggaatgcg gtcagcagcg caccggcgac ggcacccgcg
      901 gtgttcatgg catacagcaa gccgatgcgg cgtccgatct gcgcttcttc cgccaccgcg
      961 tagcgcgcca gcaccggcaa cgtggcgccc atgagcgtag tggggagggc cagcgcgacg
     1021 aaggcgctga ccagatagaa aagggtggta cccgcatggt cgctgtccgg cggagtcggc
     1081 tgccctccaa acaggctctg cagggcgacg tttgcgagcc agagcagcat cggaacgata
     1141 aaaacggcgc tgccggcgat gccgatctcc agcagtccat acgtgagtac gggtcgcgtc
     1201 acccgcgcca gaaatttttc cgcgagcgtt gcgcccagcg ccagcccgcc catgtaggcg
     1261 gcgaggacgg tggcaacggc aagttccgaa gtgccgaaga caatggcgaa ctggcgtgtc
     1321 cacgctgtct gatagatcag cgccgcgact ccgctgaaga cgaaacaccc gcacaacaat
     1381 gcgagtgcgc cgatggaaac ctgtgcaggg atgcgattga cagcttcggt ggttcgcgac
     1441 atggtcgcgg agtgtacgta tcgcggagtt ttcatggcag cctttcgcgc taacggcatc
     1501 gttacgctca ccacggattt cggcgcacgc gaaccgttcg tgggcatcat gaagggcgtg
     1561 atgctgggtc acgcgccgca cctgcggttc atcgacatgg cgcacgaagt gagcgcgttt
     1621 caacctgtcg aagccggttt ctggctcgcg cacgcattac gttactttcc cgccggcaca
     1681 ttgcacgtcg cggtcgttga cccgggtgtc ggcacgccgc gcgcactcat catcgcgttc
     1741 gcgggcgaac aggcggtgct ggcgcctgac aatggtctgt tagcgccgct ggccgcgcgc
     1801 ggcacgatcg atcgcgtcat ccaggtcgac cacgctcgcc tggtcaaatt cggcgtgagc
     1861 gacgtgagcg ccacgtttca tggccgcgat gtgttcgcgc cgctggcggc ggcaatcgcg
     1921 gcgggtcgct gcaacccctc ggacctgggg actgaaacca cgacgttcag cctggaaggc
     1981 tggcccgcga ccacgccgcg aaaggatggt ggagtggcag gggtcatcgt cgcggtcgat
     2041 cgcttcggaa acctcatctc caacatcgaa tcctcggcgg tagtcgccct gaccatgccc
     2101 atcgtgcaca tcgggggcct ggcgttgccc ctcaaacgca cctatggcga cgcccggccc
     2161 ggcgaatatc tgggggtaat caactcgttt gaggtcctgg aggttgccca gtcgcgggga
     2221 aatgccgcca aggggttgaa tctggccgtg ggggcgtcta tttcggccgt cccgggggtt
     2281 ccagaccccc aatttcggcg gggttgatat cgaacgcgaa tcggctatag ttcgcagcct
     2341 cattacgagc ccgccgggct cgtaactcac tgtttcaaat agtttttctg aagaaaaaac
     2401 gatgtccgaa cgggataacg aaagcttcga tttagacgaa gagttcctcg ccgaagctga
     2461 agacaacacg gattacgacc gcatcctgga taaggtcgat cgtcgtgttc gtcagcagcc
     2521 cggcactaaa aaagtcggca aggcagcatg gagccggctc gaagaagtgc tcgcggaccg
     2581 tcgtctcgaa aaagacctga acgacggctt cgaagacgaa cagtagattc ctgttccctt
     2641 atcagtgagt tgtcgcgcac gagcgcgacg aggttaagcg catgtccgcg cggtctgcat
     2701 cgcgctgggt ggtcgtcaaa ttcggcggca ccagcgtttc tacgctcatc aactggcgca
     2761 acatcgcgct ggtcgtcaaa cgccgtctcg attccggggc gcgcgtactg gtcgttcatt
     2821 ccgcgatcac gaagatcacc gacatgctgg agaagctgct ggcggcggcg atctccggca
     2881 atcccgagga gccgctcaag gccatcgagc agcgccatcg cgatctagct accgaactcg
     2941 gtgtcggcat gagtgcgcag cttcagggct atttcgacga actacgccag atggccaacg
     3001 gcatttcgct ggtgcgcgag ctgtccgaca agacccgcgc ccgtgtgatg gccaacggcg
     3061 aactcatggc cacggagata ggttcccgct tcctgaagtc gcagggcgtc gacgtcacct
     3121 ggatggatgc gcgcgacatg ttgtcggccg aagatcgcgg cgcctccgcc aaggcttcgg
     3181 tgttgtcggc cacctgctca ttcgcgccgg acgccgggct cgagaagcgg ctcgacgccg
     3241 cgacgccggt ggtgatcacc cagggcttca tcgcgcgcga cagcgaaggc aatacctgcc
     3301 tgctgggacg cggaggctcc gacacgtcgg cggcttacct cggcgcgaag atccgcgcaa
     3361 agcagatcga gatctggacc gacgtgcccg gcatgttcag cgccaatccg cgctcgacgc
     3421 cgaccgcgcg cctgatccgc gcgctgcatt acgacgaagc gcaggaaatc gcgaccagcg
     3481 gcgccaaggt gctgcatccg cgctgcctgc tgcccgcgcg ccagtaccag atacctttat
     3541 atgtgtacgc cacgcagacg ccggacctcg aaggcacgct gatcacgggc gagtcgggcg
     3601 ccaccgcgca ggtcaaggcg gtgtgcaatc gcaagggcat cacgctcatc tcgctcgaca
     3661 gccccggcat gtggcaccag gtcggcttca tggccgatgc attcgccgtg ttcaaggaac
     3721 acggcatgtc cgtagacctg gtgtcgacgt cggagaccaa cgtcaccgtg tcgctcgatc
     3781 cggcggccaa cacgctggac ggagagttac tgggcaaact gcagcgcgac ctgtcgaagc
     3841 tgtgcggtgt ccagatcatc gggccctgcg cctcggtgtc gctggtcggg cgcaacatcc
     3901 gcgccatcct gcacaagctc ggtgacgcgt ttgccctgtt cgaagagcaa aaggtgtaca
     3961 tggtcagcca ggcggccaac gacctgaact tcacgttcgt cgtggacgag aaccagggcg
     4021 accggctggt cgagcagctt catgacctgc tgattcatcc gctgcccggc gattcgggct
     4081 tcggtcccac ctgggaacag ttgttcgcca agcctgaagc cgtggccgcg ggcgttcagc
     4141 cgtggtgggc actgaagcgc gaggccttgt taggcaagct ggcgaaccgc gacagcgcct
     4201 acgtctacga cctggaccag gtcgaaacgg ccgcgagggc cattgccggc atcaagtcca
     4261 tcggtcgcgc gttgttcgcg atgaaggcca acccgcatcc cgacatcgtg cgcaaggtcg
     4321 ccgccgcggg tctcggattc gactgcgtat cgatcaacga gatccagcac gtgctcgcgt
     4381 cggtaccggg catctcgcct gagcgaatcc tgtttacgcc gaacttcgcg ccgcgtgccg
     4441 aatacgagcg cgcgctcgag cttggcgtgc acgtgaccat cgacaacctg ttcgtgctgc
     4501 agcactggct cgatgccttc aagggcaaga gcgtgttcct gcgcatcgat accggcattg
     4561 gccgcggcca tcaccaccat gtgaagaccg ccggtgcgga atcgaagttc ggcattccgt
     4621 ccgaagagct cgacgaagtg gcgcgccttg cgaaggcggg cgatgtgacc gtcaacggtc
     4681 tgcatgcaca tgccggcagc ggcgtgttcg acgtcgagaa ctggacgcag gtgggttcgg
     4741 tgctcgtcgg cctcgtgaag cgattcccgg acgtgaagtt catcgatgtg ggcggcggac
     4801 tcggtgttcc ggatcgcgct cagggccgcg aactggacct cgcgcggctc gatgcggcgc
     4861 tcgccaaggt cgcggcggcg attcccggcg tcacgctatg gatcgagccc ggacgatatc
     4921 tcgtggcaaa cgccggcgtg ctgttagccc gcgtgacgca gaccaagatc aagagcggca
     4981 tccgctacgt tggcgtggcc acgggcatga attcgctgat tcggccggcg ttgtacggcg
     5041 cgcatcacga catcgtgaac ctcacccgtt atggcgagcc agcgaacatc tcggcgaatg
     5101 tcgtgggtcc gatctgcgag agctcggatt tcctcgggca tgaccgcttg ctgccggaat
     5161 gtcagtcagg tgatgtgttg ctgatcgcga ccgcgggtgc ctatggccac gcaatgtcgt
     5221 ccagctacaa cttgcgcgcg ccggccgaag agctgctgct gaagtagtca gtcgggcggg
     5281 gtcgcggcat cgccgggcgt gtcgagcgtc gactggatgc gcgacattct ctgcacggcg
     5341 tcgtttcttt cgcgttcccg cctcaacatg gtgtcgagcg tttcgagttg agaacgtgaa
     5401 aggatcgcgg cggcggagct gtgcatgcgg ccgtgcgttg cctggagttg ttcggcatag
     5461 atctcggcgt acttgcgagc cgttgcgtcc gagatgtcat tcggatcggc cgcatcgctg
     5521 attgccgcga gctcccgctc catctgcgcc cgttccgcct gcagcgcggc gatcagcggt
     5581 tccacctggt cgtcacgaag gacattcacg cccgtgagtt cgttgcgcaa ttggttgacc
     5641 tggttacgag tgggccggct gtcgtaatag gcctgaaggc gggcgacgcc atctgctccg
     5701 agcacttcgc gaagtttggc ctggtgcgca acttcagcgg cttcgtcttc tgccaggctc
     5761 gaatcagtac gttccaaccc tctcaattgg ccgtcgatcg agagatcgat gaccttgtcg
     5821 gcttgttgcg gagtaaatcc caccagcttg atcaggtctt cacgtagcca ggctcgcgcc
     5881 aggcggcgct cctcgcgcca tgcctcgcgg tacttcgggt cgcgaaggtg gcggcgctcg
     5941 gcagcttcca ggtcctcttc ctcttgcgtg agttgcgagg agtatccggg ggcgccgtcg
     6001 gactcaggat cgaccgcagg cagcggcgtg atcgccggct ttcgaacctg cggcgcttct
     6061 gaaacggaca cggtgcgtac ttcgcccgcg gcgtcgagtc gcgccgagag ttcggcgttg
     6121 cgcgcgcgct cggcgcgcaa ctccatccag agtgaaacgg aaaccgcggc gctcgccacg
     6181 gctactacgg agagcaagac cgacgacttc cgcatcagga cctcggcgct cagcgcggtg
     6241 tgtgacgcga ctcgagctgg ctgactacct gcgacaacga gactccgcgc gcacgcagca
     6301 gcaccagcat gtggaacagc aggtcggccg attcctcgat gaccttgtcg tcaccctggg
     6361 ccgcaccggc cagcgcgagc tccacgcctt cttcgccgac tttctgcgcg acgcggttga
     6421 tgcccttgtc gagcagtttg gcggtgtagc tggcctcggg tttctcggcg gcgcgctgcg
     6481 caatgatgcc ttcgagcttc gcgaggaacg cgatcggcgt gacggccgcg cgggattcat
     6541 tgccgaagca ggtgagggtg ccgttgtggc aggccgggcc gcgcgggcgc gccgtgacga
     6601 gcaatgtgtc ctggtcacag tcgagtgcga tgtcgatgac gtcgagcgcg tgcccggtgg
     6661 tctcgccttt ctcccacaag cgtcgcttgc tgcggctgaa gaacacggct cgcttgcgcg
     6721 cgatagtttg ctccagcgcc tcgcgattca tgtacgccag catgagcacg gcgccggtgt
     6781 cggcgtcctg cacgatggcg ggaacgagtc cttcgccttt cacgaaatcc acgcgggcga
     6841 tgtccaatgc ggtgctcata tgcgcatctc gatgccggac gccgccagcg tggtcttgag
     6901 atcgggaatg gcgatggcgc cggagtggaa cacactggcc gccagcgcgg cgtcgaccga
     6961 tgcctgctcg aagacgtcga tgaagtgctg cggcgcgccg gcgccgcccg atgccacgag
     7021 cggcacgtcg cagacttcgc gcacggcctt caattgcgcg acgtcgtatc cgcgccgcac
     7081 gccatcgttg gtcatgcagt tgagcacgat ctcgccggca ccacgctcct ggacttcctt
     7141 cacccaggaa agcgtctcgc ggcccgtgtt gcgcgaacga ttcgggtccc cggtgaactg
     7201 gaatacgcgg tagccctccg cgaggctggc gctgtcgatg ccgatgacca cgcattgcga
     7261 accaaagcgc gacgccagct cttcgatcag acccggattg gcgatcgcgg gagagttgat
     7321 ggagatcttt tcggcaccgg cattgagcac cgcttcggcg tcttccaccg agcggatgcc
     7381 gccggccacg cagaacggga tgtccaggat gcccgcgacg cggctcaccc atttgcggtc
     7441 cacgctccgc ccttcgggac tcgcggtgat gtcgtagaac accacctcat cggcgccttc
     7501 gttccggtag cgc
//
LOCUS       DASQJS010000277         4714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_170603, whole genome shotgun sequence.
ACCESSION   DASQJS010000277 DASQJS010000000
VERSION     DASQJS010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170603"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VEW08_11135"
     CDS             <1..650
                     /locus_tag="VEW08_11135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYJ41337.1"
                     /translation="EARLPGLLTRITSLPDELCYEVQPENLKEVCLSLRDSPELRFEI
                     LIDVAGIDYLDYGTTEWKTYSATASGFSRGVNRDAPRKPHDGARFAVAYHLLSITHNQ
                     RVRLRSRCVDAEDPIIDSVVDIWPGANWFEREAFDLFGILFTGHPDLRRILTDYGFIG
                     HPFRKDFPLIGNVEVRYDADKQRVVYEPVTIEPRILVPKVIRDDNRYDAALKSQK"
     gene            654..1907
                     /locus_tag="VEW08_11140"
     CDS             654..1907
                     /locus_tag="VEW08_11140"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HYJ41338.1"
                     /translation="MNEIRNFTMNFGPQHPAAHGVLRLVLEMDGEVIQKADPHIGLLH
                     RGTEKLAESKPFNQSIGYMDRLDYVSMMCNEHAYVMAIERLLGVTVPVRAQYIRVMFD
                     EVTRVLNHLMWLGAHALDIGAMTVFLLAFKEREDLMDCYEAVSGTRMHATYYRPGGVY
                     RDLPDSMPKYQASKWRSQKNADSMNEARQGSLLDFLDAFVARFPACVDEYETLLTDNR
                     IWKQRTVNIGVVTPERAMALGFTGAMLRGSGIVWDLRKTQPYEVYADMDFDIPVGVNG
                     DCYDRYLVRIEELRQSNRIIKQCIEWLRRNPGPVMIDDRKVRPPSREAMKGDMESLIH
                     HFKLFTEGYSVPEGETYAAVEAPKGEFGIYLISDGANKPYRLKCRAPGFAHLSALEEM
                     TRGHMLADVVAVIGTQDIVFGEVDR"
     gene            1904..2446
                     /locus_tag="VEW08_11145"
     CDS             1904..2446
                     /locus_tag="VEW08_11145"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HYJ41339.1"
                     /translation="MTHVVKTADGGSFEPVLGEKARVLNDHTRHEIDEWIGRFPTGRQ
                     RSAVLSALRFTQEQNNGFLTPELMDGVAEYLRLPAIQVYEVASFYSMFETHPCGRHHV
                     SICTNISCMLNGAEDLVAHCEKKLGIKLGESTADGRIFLKKEEECLAACTGAPMMMVD
                     HEFHEYLTPEKVDKVLDELK"
     gene            2455..3780
                     /gene="nuoF"
                     /locus_tag="VEW08_11150"
     CDS             2455..3780
                     /gene="nuoF"
                     /locus_tag="VEW08_11150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HYJ41340.1"
                     /translation="MNAAEKKWADKMNLTVFEPLKLPESWKLENYLKIGGYEAWKKIL
                     AEKPTRMSVVDQVKASGLRGRGGAGFPTGTKWSFMPRDATMQKYAVCNSDESEPGTCH
                     DRDILRFNPHSLVEGMAIGGFCMNATVGYNYIRGEFLGEPVPRFEAAVKEAYEAGLLG
                     KNILGSGIDFDLYTAVGAGAYICGEETALLDSLEGKQGKPRFKPPFPAAYGLYGAPTT
                     INNTQSYAAVPTILRKGADWFAKLGVANAGGTAIFSVSGHVNKPGNYELPMGIPFADL
                     LEIAGGVKDGRKLKACIPGGSSVYVLPADKIMAANMDYDSLKAAGSSVGSAAVIVMDE
                     TTCMVKVLERLSRFYQSESCGQCTPCREGTGWMNRVIRRIMAGQGNKSDLQLLVDVAG
                     RIEGHTICALGDAAAWPVQSFIKHFRHEFEYMIDNGGRSIVDDPAKRAA"
     gene            3777..>4714
                     /gene="nuoG"
                     /locus_tag="VEW08_11155"
     CDS             3777..>4714
                     /gene="nuoG"
                     /locus_tag="VEW08_11155"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HYJ41341.1"
                     /translation="MSAVPPVSPPAAPPSDVVNVEVDGKPCQGKKGQMIIHVTDAQNA
                     YVPRFCYHDKLPIAANCRMCLVEVEKAPKPLPACATPIGEGMKIFTKSPKAIGAQKAT
                     MEFLLINHPLDCPICDQGGECELQDLAMGFGRDAARFNERKRVVKDKDLGPLVSTDMT
                     RCIHCTRCVRFGQDIAGIQELGTTGRGENMEIGTYVERAVEHELSGNIIDLCPVGALN
                     SKPFRYRARSWEMTQAALVSPHDGVGTNLYGHVLRGKLMRVVPRPNDAINETWISDRD
                     RYSYEGIYSADRLEAPMVREAGEWKRVSWEVALETAA"
BASE COUNT          943 a         1475 c         1469 g          827 t
ORIGIN      
        1 tcgaggcccg cctgccgggc ttgctcacgc gcataacgtc gctccccgat gagttgtgct
       61 acgaagtgca gccggagaac ctgaaagaag tgtgcctgag cctgcgggac tcgcccgagc
      121 tcagattcga aatcctcatc gacgtcgctg gcatcgacta cctcgattac ggcacgaccg
      181 agtggaagac gtattcggcc acggcttcgg ggttcagccg cggcgtgaat cgcgacgcac
      241 cgcgcaagcc gcatgacggc gcgcgtttcg ccgtcgcgta tcacctgctg tcgatcaccc
      301 acaaccagcg cgtgcgcctg cgttcgcgct gcgtcgatgc cgaagacccc atcatcgatt
      361 ccgtggtcga catctggccc ggcgccaact ggttcgagcg tgaggcgttc gatctgttcg
      421 gcatcctgtt caccggtcac ccggatctgc gccgcatcct cacggactac ggcttcatcg
      481 ggcatccgtt ccgcaaggat ttcccgctca tcggcaacgt cgaagtgcgc tacgacgcgg
      541 acaagcagcg cgtggtgtac gagcccgtga ccatcgagcc gcgcatcctc gtgcccaagg
      601 tcattcgcga tgacaaccgt tacgacgcgg ccctgaaaag ccaaaaataa gaaatgaacg
      661 agatccgcaa tttcacgatg aacttcggtc cgcagcatcc ggcggcgcac ggcgtactgc
      721 gcctggtgct cgagatggac ggcgaggtca tccagaaggc cgatccgcac atcggcctgc
      781 ttcatcgcgg caccgaaaag cttgccgaat cgaagccctt caaccagtcc atcgggtaca
      841 tggaccggct cgattacgtg tcgatgatgt gcaacgaaca tgcgtacgtc atggccatcg
      901 agaggttgtt aggcgtcacc gtgccggtgc gcgcgcaata catccgcgtg atgttcgacg
      961 aggtgacgcg cgtgctgaac cacctcatgt ggctcggcgc gcacgcgctc gatatcggcg
     1021 ccatgaccgt cttcctgctc gccttcaagg aacgcgaaga tctcatggac tgctacgagg
     1081 cggtttccgg cacgcgcatg catgccacgt actaccgccc gggtggcgtc tatcgcgacc
     1141 tgccggattc gatgccgaag taccaggctt cgaagtggcg ctcgcagaaa aacgccgatt
     1201 ccatgaacga ggcgcgccag ggttcgctgc tcgatttcct cgatgcgttc gtcgcgcgct
     1261 tcccggcctg cgtcgacgaa tacgaaacgc tgctcaccga caaccgcatc tggaagcagc
     1321 gcaccgtgaa cattggcgtc gtcacgccgg agcgcgcgat ggcgctgggt ttcaccggcg
     1381 ccatgctgcg cggttcgggc atcgtctggg acctgcgcaa gacgcagccc tacgaggtct
     1441 acgcggacat ggatttcgac atcccggtcg gcgtgaacgg cgactgctac gaccgttacc
     1501 tggtgcgcat cgaagagctg cgccagagca atcgcatcat caagcagtgc atcgaatggc
     1561 tgcgccgcaa tccaggtccc gtgatgatcg acgaccgcaa ggtgcgtccg ccttcacgcg
     1621 aagcgatgaa gggcgacatg gaatcgctga ttcaccactt caagctgttc accgagggct
     1681 attcggtgcc cgagggcgaa acgtacgccg cggtcgaagc accgaagggc gaattcggca
     1741 tttacctgat ttcggacggg gccaacaaac cctatcgcct gaagtgccgc gcgcccggtt
     1801 ttgcgcacct gtcggcgctc gaagagatga cgcgcgggca catgctcgcc gacgtcgtcg
     1861 cggtcattgg aacgcaggac atcgtgttcg gagaggtgga tcgatgacgc atgtcgtgaa
     1921 gacggcggat ggtggcagct tcgagccggt actcggcgag aaggctcgcg tgctcaatga
     1981 tcacactcgt catgaaatcg acgagtggat cgggcgtttc cccaccggcc gccagcgctc
     2041 cgccgtgctg tcggcattgc gattcacgca ggaacagaac aacggtttcc tgacgccgga
     2101 actcatggat ggtgtcgcgg aatacctgcg gctgccggcc atccaggtat acgaagtcgc
     2161 gagcttctat tcgatgttcg agacgcatcc ctgcggccgg catcacgtca gcatctgcac
     2221 caacatcagc tgcatgttga acggcgccga agatctcgtc gcgcattgcg agaagaagct
     2281 cggcatcaag ctcggcgaaa gcaccgccga cggccgcatc ttcctcaaga aggaagaaga
     2341 gtgtctcgcg gcctgcaccg gcgcgccgat gatgatggtc gatcatgaat tccacgagta
     2401 cctgacgcct gagaaggtgg acaaggttct cgacgagttg aagtgagcag ccgaatgaac
     2461 gcagcggaaa agaaatgggc tgacaagatg aacctcacgg tgttcgaacc gttgaaattg
     2521 ccggagtcgt ggaagctcga gaactatctc aagattggcg gctacgaggc gtggaagaag
     2581 attcttgccg agaagccgac gcgcatgagc gttgtcgatc aggtgaaggc atcgggcttg
     2641 cgcggccgtg gcggcgcggg ctttcccacc ggcaccaagt ggagcttcat gccgcgcgac
     2701 gcgacgatgc agaagtacgc ggtctgcaat tcggacgaat ccgaacccgg cacctgccac
     2761 gatcgcgaca tcctgcgctt caacccgcac tcgctcgtcg agggcatggc gatcggcggc
     2821 ttctgcatga atgccacggt cggctacaac tacatccgag gtgaattcct gggcgaaccc
     2881 gtgccgcgct tcgaagcggc ggtcaaggaa gcctacgagg ccgggttgtt aggcaagaac
     2941 attctcggct cgggaatcga tttcgatctc tacacagccg tcggggctgg cgcctatatc
     3001 tgcggcgagg aaaccgcgct gctcgattcg ctcgagggca agcagggcaa gccgcgcttc
     3061 aagccgccgt tcccggctgc ctatggcctg tacggcgcgc cgaccacgat caacaacacg
     3121 cagagttatg ccgcggtgcc cacgatcctg cgcaaaggcg cggactggtt cgcgaagctc
     3181 ggcgtggcga acgccggtgg cacggccatc ttctcggtct cgggccacgt caacaaaccc
     3241 ggcaactacg aactccccat gggcattccg tttgccgacc tgctcgaaat cgccggtggg
     3301 gtgaaggacg gccgcaagct caaggcctgc attcccggcg gctcgtcggt gtacgtgctg
     3361 cccgccgaca agatcatggc cgcaaacatg gactacgact cgctcaaggc cgcgggttca
     3421 tccgtggggt cggccgcggt catcgtcatg gacgaaacca cctgcatggt aaaagtgctc
     3481 gagcgcctgt cacgcttcta tcagagtgag tcttgcggcc agtgcacccc gtgccgcgaa
     3541 ggcacgggct ggatgaaccg cgtcattcgc cggatcatgg ccgggcaggg caacaagtcc
     3601 gatttgcagt tgttggtgga cgtcgcgggc cgcatcgaag gtcacaccat ctgcgcgctg
     3661 ggtgatgccg ccgcgtggcc ggtacagagt ttcatcaagc acttccgcca cgaattcgaa
     3721 tacatgatcg acaacggcgg gcgaagcatt gtcgacgacc cggcgaagag ggcggcatga
     3781 gcgcggttcc tcccgtctcg ccgcccgcag cgccgccgtc cgacgtcgtc aacgtcgaag
     3841 tcgacggcaa gccctgccag ggcaagaaag gccagatgat catccacgtc acggacgcgc
     3901 agaacgcgta cgtgccgcgc ttctgctacc acgacaaact acccatcgcg gccaattgcc
     3961 gcatgtgcct cgtggaagtg gagaaggccc cgaagccgtt gccggcctgc gccacgccca
     4021 tcggcgaggg catgaagatt ttcaccaaga gcccgaaagc cattggcgcg cagaaagcga
     4081 ccatggagtt cctgctcatc aatcacccgc tcgattgccc gatctgcgac cagggcggcg
     4141 agtgcgagct gcaggatctc gcgatgggct tcggccgcga tgcggcgcgt ttcaacgaac
     4201 gcaagcgcgt ggtgaaggac aaggatctgg gtccgctggt ttccaccgac atgacgcgtt
     4261 gcatccattg cacacgctgc gtgcgcttcg gccaggacat cgcgggcatc caggagctcg
     4321 gcacgacggg tcgtggcgag aacatggaaa tcggcaccta cgtcgaacgc gccgtggagc
     4381 acgagctctc gggcaacatc atcgacctgt gcccggtggg tgcgctcaat tccaagccgt
     4441 tccgttatcg cgcgcgctcg tgggaaatga cccaggcggc gctcgtgtcg ccgcacgatg
     4501 gcgtcggcac caatctctat ggccatgtgc tgcgcggcaa gctcatgcgc gtggtgccgc
     4561 gtcccaacga cgccatcaac gaaacctgga tctccgatcg cgatcgctac agctacgaag
     4621 gcatctactc ggccgaccgc ctcgaggcgc cgatggtgcg cgaagccggc gaatggaagc
     4681 gcgtgagctg ggaagtcgcc ctcgagacgg cggc
//
LOCUS       DASQJS010000278         5423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3621767, whole genome shotgun sequence.
ACCESSION   DASQJS010000278 DASQJS010000000
VERSION     DASQJS010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3621767"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VEW08_11160"
     CDS             complement(<1..712)
                     /locus_tag="VEW08_11160"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HYJ41342.1"
                     /translation="MSKYRHRLPQLRNTLFLTDGGLETTLLFHDKVDLPYFAAFDLLR
                     TPTGVERLRAYFETYSRIALERRAGIVLEAPTWRANADWAAKLGYDTAALADANRRGV
                     GLLLEVRDALETPATPIVISGNIGPRGDGYRADARMSIADALRYHDAQVATFAATDAD
                     MIGAFTMNYVEEAVGIAMAARAHAIPLALSFTVETDGKLPSGQPLVEAIERVDAETGE
                     YPVYYMINCAHPSHFEDVL"
     gene            complement(709..1467)
                     /locus_tag="VEW08_11165"
     CDS             complement(709..1467)
                     /locus_tag="VEW08_11165"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HYJ41343.1"
                     /translation="MPTPLELLLDPLTLSVIAIFAALVLWEALFPARALPRVRAWRTR
                     GLLAFVAYMAISSYLPFAWAEWLAPLQLFDLSGLGALAGATLGVLLYEAGVWAWHRSL
                     HASNALWRYSHQWHHSAERLDTWGAFWFSPLDMAGWTALFSLALTLIGLSPPAVFATV
                     CVTTFLSVFQHANVRTPRWLGVLVQRPESHSWHHARGVHAGNYSDLPLFDMLLGTWHN
                     PRDFAPEQGFHRGASSQYGSLLLGRDVAAYGEPS"
     gene            1568..2605
                     /locus_tag="VEW08_11170"
     CDS             1568..2605
                     /locus_tag="VEW08_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ41344.1"
                     /translation="MDTRNRPASVAILALPEVGVSTVYGMFDMFMSAGRDWGVIVDGT
                     PGNSLIAPRVIANTREAFAAGNGVIIRADATLDEAGVPDVVCVPELLVAPGEPIDGRF
                     RAEVEFLRRCHDAGATVATACSGALLLAEAGLLDGHDATTHWAYCDILQRRYPRVRVH
                     AQRALVVAGAEQRLVMAGGGTSWLDLALYLIARLAGVEAAMQVARLNLIDWHAVGQQP
                     FARLARSRQTDDSVIARCQEWIAGNYAAAGPVAQMTRLSGLAERSFTRRFREATGMAP
                     LEYVHTLRLEEAKQMLETCEQPVEGIANEVGYEDAAFFSRLFRRKVGLTPVQYRRRFG
                     SLRRSLTSGNA"
     gene            2648..3454
                     /locus_tag="VEW08_11175"
     CDS             2648..3454
                     /locus_tag="VEW08_11175"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HYJ41345.1"
                     /translation="MSWLEAIILGLVEGITEFLPVSSTGHLILTQALLGLKGAAVERH
                     IIIIQGAAILAVCWEFRKKLLHTAFSLHSDPVSRRFLLNLFIASIPLAVLGLKFEDQI
                     KAFLFNPVTVAIALVVGGVIILWAERRKHEERVQSVDELTAMDAVKLGLFQALALIPG
                     TSRSGATIIGGLLSGLSRRTAAEFSFFLAIPALLAATAYDIWKARAEFASADVEPLIV
                     SCVASFISALLVTKLLLQFVSKHSFAAFAWYRIAFGAIILVTAYTGIVHW"
     gene            3528..3794
                     /locus_tag="VEW08_11180"
     CDS             3528..3794
                     /locus_tag="VEW08_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYJ41346.1"
                     /translation="MKFQFEKFYQAVNAKREERAASWRQVAREIGIGKSTLARMAQNK
                     RPDADGLAALSFWAELNPADFVTREQPAVSAVMECGVGASAISA"
     gene            complement(3791..4546)
                     /locus_tag="VEW08_11185"
     CDS             complement(3791..4546)
                     /locus_tag="VEW08_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HYJ41347.1"
                     /translation="MNVLIVDDEPLARAGMRLLLDEESAISSIAEARNGAEAVELVRA
                     RRPDLLLLDVQMPEMDGFGVLKALGAEQMPPVIFVTAHDKYAIQAFEVNAIDYLLKPV
                     TRERFQQALARVRERVSTQGLDNQHVLSLLQQLSAQPKYLARVALRSAGKISFVSIED
                     VLYVQAAENYVQLHLRASRHLLHVPIATLEASLDPKMFLRIHRSLVVNVRHIQELETG
                     PHGEYIVVLHGGTKLQSSRSYHEKIKRWAANPF"
     gene            complement(4543..>5423)
                     /locus_tag="VEW08_11190"
     CDS             complement(4543..>5423)
                     /locus_tag="VEW08_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYJ41348.1"
                     /translation="SIAWELLRLSLEAAFHLTWNEFWPIQPPITVKSEFTLLFIFGFH
                     TGVVTYWVVLSIQTAIRNYARFQERAQAALRSDLRASQLETQVAQARLGALKAQLQPH
                     FLFNTLNAIVVLVRQQKGQQAEETLARFSDLLRAVLADMDVQEVSLARELEYLKLYLS
                     IEQLRFSDRLRVEFDVDPDMLDAAVPHMALQPLVENSIRHGVGQRSTPGVIIIRASRV
                     GDALHVVVQDDGPGFGAAGAGGGLKLGLANTRARLNQLYGEASELRTEAAPGGGALVT
                     MVLPFHLVQDSGVEAT"
BASE COUNT          967 a         1749 c         1783 g          924 t
ORIGIN      
        1 ccaaaacgtc ctcgaagtgc gagggatgcg cgcagttgat catgtagtac accggatatt
       61 cgccggtctc ggcgtcgacg cgctcgatgg cctccaccag gggctggccg gacggtagtt
      121 tgccgtcggt ctccacggtg aacgacagcg cgagcgggat cgcgtgcgcg cgcgccgcca
      181 tcgcgatgcc tacggcctcc tccacgtagt tcatggtgaa agcgccgatc atgtcggcgt
      241 cggtcgccgc gaaggtcgcc acctgcgcgt cgtgatagcg aagcgcatcg gcaatgctca
      301 tgcgtgcgtc ggcgcggtag ccgtcgccgc gcgggccgat gttgccgctg atcacgatgg
      361 gtgtcgcggg tgtctcgagc gcgtcgcgca cctccagcag caggccgacg ccgcggcggt
      421 tcgcatcggc cagcgccgcc gtgtcgtaac ccagcttggc ggcccaatcg gcattcgcgc
      481 gccacgtggg cgcttcgagc acgatgcccg cgcggcgctc gagcgcgatg cgtgagtagg
      541 tctcgaaata cgcgcgcaga cgctcgacgc cggtcggtgt acgcagcagg tcgaaggccg
      601 cgaagtacgg gaggtcgacc ttgtcgtgaa acagcagcgt ggtctcgagc ccaccgtcgg
      661 tgaggaacag cgtattgcgc aactgcggca gacggtgacg atacttgctc atgacggttc
      721 tccatatgcc gcgacatcgc ggccgagcag cagactgccg tactgcgacg aagcgccgcg
      781 gtggaagccc tgctcgggcg cgaagtcgcg cgggttgtgc caggtgccta acaacatgtc
      841 gaacagcgga agatcggaat agttcccggc gtgcacgccg cgcgcgtggt gccacgaatg
      901 gctctccggg cgctgcacca gcacgccgag ccaccgcggc gtacgcacgt tggcatgctg
      961 gaacacggag aggaacgtgg tcacacagac cgtcgcgaac accgccgggg gcgacaggcc
     1021 aatgagcgtg agcgcgaggc tgaacaacgc cgtccagccg gccatgtcga gcggactgaa
     1081 ccagaacgcg ccccaggtat cgaggcgttc ggcgctgtga tgccactggt gcgagtagcg
     1141 ccacaacgca ttcgacgcgt gcaggctgcg gtgccaggcc cagacgccgg cttcgtagag
     1201 cagcaccccc agagtcgcgc cggccagcgc tcccaggccc gacaggtcga atagctgcag
     1261 cggcgcgagc cactccgccc aggcgaacgg cagatacgac gagatggcca tgtaagccac
     1321 gaacgccagc agtcccctgg tccgccaggc gcggacgcgc ggcagtgcgc gcgccgggaa
     1381 tagcgcttcc cagagcacga gcgcggcgaa aatcgcgatc acggaaagag tgagagggtc
     1441 gagcagcagc tcgaggggcg tgggcatgga aatctccttg gcaggcttgt cttgaagggc
     1501 gtgcaaggta gagtgcgagc cgcgaaatca ctcaagccgt aacggccacg aacctgacga
     1561 ttccgccatg gatacccgga acagacccgc cagcgtcgcg atcctcgctc taccggaagt
     1621 gggtgtgtcc acggtctacg gcatgttcga catgttcatg tccgcggggc gggactgggg
     1681 agtcatcgtc gatggaactc ccggtaactc gctgatcgcg ccacgcgtca tcgccaacac
     1741 gcgagaggcg ttcgccgcgg gaaacggcgt gatcatccgc gcggatgcca cgctcgacga
     1801 agcgggtgtg ccggacgtcg tgtgcgtgcc cgaactgctg gtggcgcccg gcgagcccat
     1861 cgacggccgg tttcgcgccg aggtcgagtt tctcaggcgc tgccacgatg ccggtgccac
     1921 ggtggccacc gcctgctccg gtgcattgct gctggccgag gccgggttgt tagatggcca
     1981 cgacgcgacc acgcattggg cctattgcga catcctgcag cggcgttatc caagggttcg
     2041 cgtgcacgcg caacgcgcgc tggtggtggc tggcgcggag caaaggctgg tgatggcggg
     2101 tggcggaacg tcctggctcg acctggcgct gtatctcatc gcgcggctcg cgggcgtcga
     2161 ggcggcgatg caggtggcgc gcctcaatct gatcgactgg cacgccgtcg gccagcaacc
     2221 gttcgcacgc ctggcgcgtt cacgccagac cgacgactcg gtcatcgctc gttgccagga
     2281 atggatagcc gggaactacg ccgcggccgg acccgtggca cagatgacgc ggctgtccgg
     2341 gctcgccgaa cgctcgttca cgcggcgatt ccgcgaagcc acaggaatgg cgccgctcga
     2401 atacgtgcac acgctgcggc tcgaagaagc caaacaaatg ctggaaacct gcgaacagcc
     2461 ggtggaaggc atcgccaacg aggtgggcta cgaagacgca gcgtttttct cccggctgtt
     2521 ccggcgcaag gtgggactca ccccggtcca gtatcgtcgc cgcttcggca gtttacggcg
     2581 cagtctgacg agcgggaacg cttgagcctc cgctaaactg cgcgccccga actgaacaag
     2641 gtcgcgcatg tcctggctcg aagcgatcat cctgggcctc gtcgaaggca tcaccgaatt
     2701 cctgccggtc tccagcactg gacacctgat cctcacgcag gcgttgttag gcctgaaggg
     2761 cgcggcggtc gaacgtcaca tcatcatcat ccagggcgcg gcgattctcg cggtgtgctg
     2821 ggagttccgg aagaagctgc tgcacacggc gttctcgctg cacagcgacc cggtgtcgcg
     2881 tcgcttcctg ttgaatctct tcatcgcctc gatcccgctc gcggtgctgg gcctgaagtt
     2941 cgaggatcag atcaaggcgt tcctgttcaa cccggtcacg gtggcaatcg cgctggtggt
     3001 cggcggcgtc atcatcctgt gggccgagcg gcgcaaacac gaagaacgcg tgcagtcagt
     3061 cgacgaactg actgccatgg acgcggtgaa gctggggctg ttccaggcgc tcgcactgat
     3121 tcccggcacc agccgctcgg gcgcgacgat catcggcggt ttgctgagcg gcctgtcgcg
     3181 gcgcacggcg gcggaatttt cgttcttcct ggccattccg gcattgctgg cggccaccgc
     3241 gtacgacatc tggaaggcgc gagcggagtt cgccagcgcc gatgtcgagc cgctcatcgt
     3301 cagttgcgtg gcgtctttca tcagcgcact gctggtcacc aagctgctgc tgcaattcgt
     3361 cagcaaacat tcgttcgcgg cgttcgcgtg gtaccgcatc gcgttcggcg ccatcatcct
     3421 ggtcaccgcg tacaccggca tcgtgcactg gtagctcgtc accggatttc tccacttcgt
     3481 caaaattccc ttttccgggt catcgcgcgg gggcgagact cgggtgcatg aagttccaat
     3541 tcgaaaagtt ctaccaggca gtcaatgcga agcgcgaaga gcgcgcggcc tcgtggcgcc
     3601 aggtggcgcg cgagatcggc attggcaaat ccacactggc gcgcatggcg cagaacaagc
     3661 gtcctgacgc tgacggcctc gcggccctgt cgttctgggc ggagctcaac cccgcggatt
     3721 tcgtcacgcg cgagcagcct gcggtgagcg ccgtgatgga atgcggcgtg ggcgccagcg
     3781 caatcagcgc ctagaacgga ttcgccgccc agcgcttgat cttctcgtga tagctgcggc
     3841 tcgactgcag ctttgtcccg ccgtgcaaca ccacgatgta ttcaccgtga ggcccggtct
     3901 ccagctcctg gatgtgccga acgttcacca ccagtgaacg atgaatgcgc aggaacatct
     3961 tcggatcgag tgaggcttcg agcgtggcga tcggcacgtg cagcaggtgg cgcgaggctc
     4021 gaaggtgcag ctgtacgtag ttctccgccg cctgcacgta cagcacgtct tcgatgctga
     4081 cgaaggaaat ctttcccgcg gatcgaagcg ccacccgcgc gaggtatttc ggctgcgcgg
     4141 acaactgctg caatagtgac agcacgtgct ggttgtccag gccttgcgtg ctcacgcgct
     4201 cgcgcacacg cgccagcgcc tgctggaagc gctcccgcgt caccggcttc aacagatagt
     4261 cgatggcgtt cacctcgaac gcctggatcg cgtacttgtc gtgcgccgtg acgaatatca
     4321 ccggcggcat ctgctccgca cccagcgctt tcagcacacc gaagccgtcc atttcgggca
     4381 tctgcacgtc gagcagcagc agatccggtc gacgcgcccg tacgagctcg accgcttctg
     4441 cgccattgcg cgcttcggcg atcgacgaga tcgccgattc ctcatcgagc agcaggcgca
     4501 tgccggcacg cgccagaggc tcgtcatcga cgatcagaac gttcatgtcg cttccacgcc
     4561 ggaatcctgg acgaggtgaa acgggagcac catcgtaacg agtgcaccgc cacccggcgc
     4621 cgcctcggtg cgcaattccg aggcttcgcc atacagttga ttgagacgcg cgcgcgtgtt
     4681 ggcaagcccc agcttgagac cgccgcccgc tcctgccgcg ccaaaccccg gcccgtcatc
     4741 ctgcaccacc acgtgcagcg catcgccgac acgtgatgcg cgaatgatga tgacgccggg
     4801 cgtggatcgc tggcccacgc cgtgccggat ggagttctcg accaggggct gcagcgccat
     4861 gtgcgggacg gccgcatcga gcatgtcggg gtccacgtcg aactcgacgc gcagcctgtc
     4921 ggagaacctc agctgctcga tggatagata gagcttgagg tactcgagct cgcgcgccag
     4981 cgagacttcc tggacatcca tgtcggcgag aaccgctcgc agcaggtcgc tgaaacgcgc
     5041 cagggtttcc tcggcctgct gacccttctg ctgccgcacc agcaccacga tggcgttcag
     5101 cgtgttgaac aggaagtgcg gctgcaattg cgctttcaat gcgccgagtc gggcctgcgc
     5161 cacctgggtt tcgagttgtg aggcgcgcag gtccgaacgc agcgccgcct gtgcacgttc
     5221 ctggaagcgc gcgtagttgc ggatcgcggt ctggatgctg agcacgaccc agtaggtcac
     5281 cacgccggta tgaaacccga agatgaacaa cagcgtgaac tcgctcttga cggttatcgg
     5341 cggctgaatc ggccagaact cgttccacgt gagatggaac gcggcctcca gcgacagccg
     5401 cagcaactcc caggcgatgc tca
//
LOCUS       DASQJS010000279         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2369203, whole genome shotgun sequence.
ACCESSION   DASQJS010000279 DASQJS010000000
VERSION     DASQJS010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2369203"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /gene="aceA"
                     /locus_tag="VEW08_11195"
     CDS             complement(<1..657)
                     /gene="aceA"
                     /locus_tag="VEW08_11195"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HYJ41349.1"
                     /translation="MSDRAAQQHRLSHEWSSHPRWRGIERPYSAADVVNLRGTVHIEH
                     SLALLGADKFWKLLHEEPVVGGLGCVTGNQAVQAVQAGLKAIYCSGWQVAGDGNSSGE
                     MYPDQSLYPVDSVPKMIERINNALLRTDQIHALDNVQNIDWMAPIIADAEAGFGGNLN
                     AFELTKAMIKAGAAGVHFEDQLSSAKKCGHMGGKVLVPTDEAINKLVAARLAADVSGV
                     P"
     gene            768..2249
                     /locus_tag="VEW08_11200"
     CDS             768..2249
                     /locus_tag="VEW08_11200"
                     /inference="COORDINATES: protein motif:HMM:NF023689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3612 domain-containing protein"
                     /protein_id="HYJ41350.1"
                     /translation="MGKSVRQGHFLGAKLRSLRKRNGLTLEELSARCVQIDATAAPSV
                     SYLSMIENGQRMPSDDMLALLAGIFGKSSGWFLDQNTELDAVPKPAARGGLAAMPLEP
                     SFLFAHDLLRIALPELLSQTATTGKQFAQLLIRVWQETRHNDFPDIERAAEQVGDRRM
                     PLTPNELLAICKHLGLQIKWFEGKDESTRAMVRSRFEAPNTVWINKRLKMQEPRLKYE
                     LAFFAGHRIMHNGDGLISPHEATWNESAEAAAGGTSMGPQDVLHAWRDFECGSFAAAL
                     LCPRAPFRRFLIREAHSVEAGSRLGVTSAVMMRRMTSVSPYRHWHFFDAYPPGFLRAV
                     YRANGIPLPWGNMSVVPDPCPRWAVFRMLKNPGTSAAKLAQQPKSQISIMPDGERPRL
                     YCCHSMLTRDAAAVSHVISVGVDLSPALSAQGYDESALVNSIGEACRKGGGDAPIPSA
                     AAEALRIVSKVLNIGWIGDALAHEATTICSRSNHCPRSPHACH"
     gene            2262..>2461
                     /locus_tag="VEW08_11205"
     CDS             2262..>2461
                     /locus_tag="VEW08_11205"
                     /inference="COORDINATES: protein motif:HMM:NF045177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41351.1"
                     /translation="MNTNYCERAGLRVATELALLIEQDVAPAVGLDTARFWTGFAALL
                     DELVPVNQALLVRRDQLQLDID"
BASE COUNT          431 a          775 c          787 g          468 t
ORIGIN      
        1 tggcacgcca gatacgtcgg ctgcgagtcg tgccgccacc agtttgttga tggcttcatc
       61 ggtgggcacc agcaccttgc cgcccatgtg accgcatttc ttcgcggagg agagctgatc
      121 ctcgaagtgc acgcctgcgg caccggcctt gatcatggcc ttggtgagct cgaaggcatt
      181 gaggttgccg ccgaaaccgg cctccgcatc cgcgatgatg ggcgccatcc aatcgatatt
      241 ttgcacgttg tccagcgcat ggatctgatc cgtgcgcagc agcgcattgt tgatgcgttc
      301 gatcatcttc ggtaccgagt cgacgggata cagactctgg tccggataca tctcgcccga
      361 gctgttgccg tcgccggcga cctgccagcc ggagcagtag atagccttca gcccggcttg
      421 cacggcctgc acggcctggt tgcccgtcac gcatccgaga ccgccgacaa caggctcctc
      481 gtgcagcagc ttccagaatt tgtccgcccc cagtaacgca agcgagtgct cgatgtgcac
      541 ggtgcctcgc aggttcacga cgtcggccgc cgagtaaggg cgctcgatac cgcgccagcg
      601 gggatgcgag ctccattcgt ggctcaggcg gtgctgctgc gcggctcgat cggacatgtc
      661 ggaactccgt ggctgtgatt ggtgcttgat gcgccgaggg agcgaagagt agagtgattg
      721 ccacacggat tttcacaata gaaacttcac aatgtgaatt tcacaacatg ggtaaatcag
      781 tccgacaagg tcattttctc ggcgccaagc ttcgatcgct gcgcaagcgc aatgggctga
      841 cgctcgaaga gctgtcggcg cgctgcgttc agatcgatgc gacggcggcg ccgtccgtct
      901 cgtacctgag catgatcgag aacggccagc gcatgccgtc ggacgacatg ctggccctgc
      961 tggcgggcat cttcggaaag tcttccggtt ggttcctcga tcagaatacc gagctcgacg
     1021 cggtgccgaa gccggccgcg cggggcgggc tcgcggcgat gccgctggag cccagctttc
     1081 tgttcgccca cgatctgctc cggattgcgc tgcccgagct gctgagccag acggcgacca
     1141 cgggcaagca attcgcgcaa ttgctgatcc gcgtctggca ggaaacgcga cacaacgatt
     1201 tccccgacat cgagcgcgcg gcggaacagg tgggcgaccg ccggatgccg ttgacgccga
     1261 atgaattgct ggcgatctgc aagcacctgg ggctgcagat caaatggttc gaagggaagg
     1321 acgaatccac gcgcgcgatg gtgcggtcgc gattcgaggc gcctaacacg gtctggatca
     1381 acaagcgcct gaagatgcag gaacccaggc tcaagtacga gctggcgttc ttcgccggcc
     1441 accgcatcat gcacaacggc gacggcctca tttcaccgca cgaggcgacg tggaacgaat
     1501 ccgcggaggc ggcggccggc ggcacgagca tgggtcccca ggacgtgctg catgcctggc
     1561 gcgacttcga gtgcggatcc ttcgcggccg cgctgttgtg tccgcgcgcg ccttttcgcc
     1621 gcttcctgat ccgggaggca cactcggtgg aagcaggctc ccggctcggc gtgacatcgg
     1681 cggtgatgat gcggcgcatg acctcggtct cgccatatcg tcactggcat ttcttcgacg
     1741 cctatccacc ggggtttctg cgcgcggtgt atcgcgccaa tggcattccg ctgccctggg
     1801 gcaacatgag cgtggtcccc gacccatgcc cccgctgggc ggtcttccga atgctcaaga
     1861 acccgggtac ctcggccgcg aagctggcgc agcagccgaa atcgcagatc tcgatcatgc
     1921 cggatgggga gcgcccccgg ctgtattgct gccattcgat gttgacccgc gatgctgccg
     1981 ccgtctcgca cgtcatctcg gtgggagtcg atctgtctcc ggctctctcc gcgcagggtt
     2041 atgacgagtc cgcgttggtg aattcgatcg gggaggcctg tcgcaagggc ggtggcgacg
     2101 cgccgattcc ctccgcggca gcggaagcgc tgcgaatcgt ctccaaggtg ctgaacatcg
     2161 gctggattgg cgacgcactc gctcacgaag cgaccaccat ctgctcgcgc agcaatcatt
     2221 gcccgcggtc ccctcatgcc tgccattgac caggtagaag aatgaatact aactactgtg
     2281 agcgcgcggg actgcgggtg gccacggagc tggcgctgct gatcgagcag gatgtcgcgc
     2341 cagccgtggg gctggacacc gcacgtttct ggactggatt cgccgcgctg ctggatgaac
     2401 tcgttcccgt caaccaggct ctgctcgtcc ggcgcgacca gcttcagctc gacatcgacg
     2461 a
//
LOCUS       DASQJS010000280         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2995980, whole genome shotgun sequence.
ACCESSION   DASQJS010000280 DASQJS010000000
VERSION     DASQJS010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2995980"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VEW08_11210"
     CDS             <1..782
                     /locus_tag="VEW08_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41352.1"
                     /translation="AQGLPAETLMSEYSPGQFEITLHHRDDCLRAVDEAVLFKRLLRG
                     VAQKHGLIACFMAKPFTARAGSGLHVHVSMNDAAGRNLFASDDAAGTPALRHAIGGMK
                     AVMPESLAIFAPNANSYRRFVSESYAPIAPIWGINNRSVSLRVPAGPPASRHVEHRIC
                     GADANLYLAVATVLGAAAHGLRERLDPGAPVEGNGYAQPGVRSLPGSWSEALALAATS
                     EFLEETLGREFLKIFLAIKHQECARFSAEVSELDYAWYLRS"
     gene            859..1842
                     /locus_tag="VEW08_11215"
     CDS             859..1842
                     /locus_tag="VEW08_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ41353.1"
                     /translation="MKLVNALIALLLAMPALAEPVAGNVEVTWNAGAEDCEKNPQPPL
                     QVHHYEAQTIILRQSLCADFEAPLLYLLIGSKRALLIDSGAVADAKSMPLAETVSGLL
                     PVVGGVRLPLLVVHTHRHGDHRAGDAQLAALPATQVVPVDADGMRAFFRFEQWPEGLA
                     QLDLGDRLIEVLPVPGHQSEHVLFYDSRTGIVFSGDFLLPGRLLVDDIDDYRASAQRA
                     VEFLEDKPVTHVLGAHIELAEDGEAFPGGSTFHPNERGPALGKADLESLPAALAAFNG
                     FYSSHPNFIVVNPIRILISMAAGAVVALGLLVWFARRLWKRRRVSGTRSAH"
     gene            complement(1901..>2247)
                     /locus_tag="VEW08_11220"
     CDS             complement(1901..>2247)
                     /locus_tag="VEW08_11220"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HYJ41354.1"
                     /translation="TGELGVTEPSGRLKKTLPSIEDAARFYERVTGVKAANFVNASKS
                     FGVDLKMRQADSQIIAMQVSGTPTLVVNGKYRVNNDDLTFDQIIQVVKFLVAKESGSA
                     AAPAAAAPASKP"
BASE COUNT          375 a          702 c          739 g          431 t
ORIGIN      
        1 gtgcgcaagg attgccggcc gagacgctga tgtccgagta ctcgcccggg caattcgaaa
       61 tcacgctgca tcatcgcgat gattgcctgc gcgccgtcga tgaagctgtg ttattcaagc
      121 gccttctgcg cggcgtggcg cagaaacatg ggctcatcgc ctgtttcatg gccaagccct
      181 tcaccgcgcg cgcgggcagc ggcctgcacg tgcacgtcag catgaacgat gccgccggcc
      241 gtaacctgtt cgccagcgac gatgcggcag ggaccccggc gctacgtcac gccattggcg
      301 gtatgaaggc ggtaatgccg gagtcgctgg ccattttcgc gcccaacgcg aattcgtatc
      361 gccggttcgt cagcgagagt tacgcgccga tcgcgcccat ctggggtatc aacaatcgct
      421 cggtcagcct gcgcgtgccg gcgggaccgc cggcgtcgcg acatgtggag catcgcatct
      481 gcggtgcgga cgccaatttg tacctggcgg tggccacggt gttgggcgcc gccgcgcatg
      541 gattgcgcga gaggctcgat ccgggcgcgc cggtggaagg caacggttat gcgcaacccg
      601 gcgtgcgctc gttgcctggc agctggagcg aagcgcttgc gctcgcggcg acgtccgaat
      661 tcctcgaaga aaccctgggc agggaattcc tgaagatttt cctcgccatc aagcaccagg
      721 aatgcgcgcg cttctcggcc gaggtcagcg agctcgacta cgcgtggtac ctgcgtagct
      781 agagaaaatt ctccatttct gcaaccaggc aggccggctg cacgtattga gggcagtcct
      841 ggtcgctgga gtcgttgcat gaaactcgta aatgcgttga tcgccctctt gcttgccatg
      901 cccgcgctgg cggagccggt cgccgggaac gtcgaggtca cctggaacgc cggcgccgag
      961 gattgcgaaa aaaatccgca gccgccgctg caggtgcatc actacgaggc gcagacaatc
     1021 atcctgcgcc agagcctgtg cgcggatttc gaagcgccgc tgctatatct gctcatcggg
     1081 tccaaacgcg ccctgctcat cgacagtggc gccgtcgcgg acgcaaagag catgccgctg
     1141 gccgagacgg tgtcaggttt gttgccggtc gtcggtggtg tgcggctacc gctgctcgtg
     1201 gtccacacgc accgccacgg cgaccatcgt gccggcgatg cgcagttggc cgcgttgccc
     1261 gcgacgcaag tggtcccggt cgatgcggat gggatgcggg cgttctttcg attcgaacaa
     1321 tggcccgaag gcctcgcgca gctcgatctc ggcgatcggc tgatcgaggt gctgcccgtg
     1381 ccgggtcatc agagcgaaca cgtgctgttt tacgactcgc gcaccggcat cgttttctcc
     1441 ggcgatttcc tgctgccggg gcgcctgctg gtcgacgata tcgacgacta tcgcgcgagt
     1501 gcccaacgcg ccgtcgaatt cctcgaggac aagccggtga cgcacgtgct cggtgcgcat
     1561 atcgaactcg cggaggacgg agaagcattt ccgggcggaa gcaccttcca tccgaacgaa
     1621 cgtgggccgg cgctgggcaa ggcagatctc gagtcgctgc ccgctgcgct cgcggcattc
     1681 aacggcttct actcgagcca cccgaatttc atcgtggtga atcccatccg catcctcatc
     1741 tcgatggccg cgggcgctgt tgtcgcgctc ggactccttg tctggttcgc gcgaagactc
     1801 tggaaacgac ggcgcgtcag tggcacacga tccgctcatt gaccggctgg cagaaagcct
     1861 cgaaaccgtg cacgcgccgg tggtgggcca cgaagtgtag ttagggcttg ctggcgggcg
     1921 cagctgctgc cggtgcggct gcggatccgc tttccttggc cacgaggaac ttgaccacct
     1981 ggataatctg atcgaaggtc aggtcgtcgt tgttcacgcg atacttgccg ttgaccacca
     2041 gcgtcggcgt gcccgacacc tgcatggcga tgatctggct gtcggcctgt cgcatcttga
     2101 ggtcgacgcc gaaggacttc gaggcgttga cgaaattcgc ggccttcacg cccgtgacgc
     2161 gctcgtagaa gcgcgcggcg tcttcgatgc tgggcagcgt cttcttgagg cgcccggaag
     2221 gttcggtgac gccgagctcg ccggtgg
//
LOCUS       DASQJS010000281         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_172301, whole genome shotgun sequence.
ACCESSION   DASQJS010000281 DASQJS010000000
VERSION     DASQJS010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172301"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1679)
                     /gene="nhaA"
                     /locus_tag="VEW08_11225"
     CDS             complement(<1..1679)
                     /gene="nhaA"
                     /locus_tag="VEW08_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HYJ41355.1"
                     /translation="MNNRLERAVDPERDHTLGAARAEITLVEFGSYDCPHCRAANARI
                     GEVRAQLGDRVRYVFRHRPISGSELARRAAELVEQAQTPDDFWSAHVSLMTRSRTLTE
                     DDLRAVADDLRLGENPLEVATALETRARERVDADVASSHASGVRFTPTFFINGRRYDG
                     PWDETSFTDAMLGSLGHRVRVAALGFASWAPSTGLLLLLATLLAVVLTNTDAGPAFTG
                     FWETPLSLGFGDAAFSMPLVHWVNDALLTVFFLVVGLEIKREFTVGHLASRRSAALPI
                     AAAVGGMVIPALIYVSLVPSGPWALGWGVPMATDTAFAVALIAIMGVRVPVELRIFLT
                     AAAIVDDIGAIIVVAIFYSDALNLAALAGALATVCVLALLNRSGVYRVTPYVITGVLL
                     WVCVHAGGLHATLAGVLLAAFIPTRKPPDYQALALQAEAILEAEASHGGEQFRFGPSE
                     PALRELDDIHDRLESPADRLLRNVAPRSSYLVLPIFALANAGVVIAAEVLTGHGLLMA
                     AIICGLAIGKPVGFILASWLAVKLGIAEKPAAYSWRQLAGAGALAGIGFTMS"
     gene            1820..>2068
                     /locus_tag="VEW08_11230"
     CDS             1820..>2068
                     /locus_tag="VEW08_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ41356.1"
                     /translation="MACANGGQAGIDMFGAACDGNLPFSTVITDLGMPHVDGRQVAAA
                     IKAIERTARIIMLTGWRQLLADGSDAPAHVDKILSKPPN"
BASE COUNT          410 a          688 c          670 g          300 t
ORIGIN      
        1 gacatcgtga atccgatgcc cgccagcgca ccggcgccgg ccagttgccg ccaggagtac
       61 gccgcaggct tctcggcgat gcccaacttc accgccagcc acgacgccag aatgaacccg
      121 acaggttttc caatggcgag accgcagatg atggccgcca tcaaaagtcc gtggccggtg
      181 agcacttcag cggcaatgac gacgccggca ttcgccagcg cgaatatcgg cagcaccagg
      241 tagctggagc gcggcgcgac gttgcgcagc agccggtccg ccggcgattc gaggcgatca
      301 tggatgtcgt ccagctcgcg cagcgcaggc tcggatgggc cgaagcggaa ctgttcgccg
      361 ccgtgactgg cttccgcctc gaggatggcc tcggcctgca gcgccagcgc ctggtagtcc
      421 ggcggcttgc gcgtgggaat gaacgcggct aacaacacgc ccgcgagcgt cgcgtgcaat
      481 ccacccgcat gcacgcacac ccacagcagc acgccggtga tcacatacgg tgtgacgcga
      541 tacaccccgg agcgattcag cagcgcgaga acgcagaccg tggcgagtgc gcccgcaagc
      601 gccgccaggt tgagcgcatc ggaatagaag atggcgacga cgatgatggc cccgatgtcg
      661 tcgacaatgg ccgcggccgt gagaaagatg cgcagctcca ccggcactcg cactcccatg
      721 atcgcaatca gcgccacggc gaacgccgta tccgtggcca tcggcactcc ccagccgaga
      781 gcccagggcc cgctgggcac cagcgacaca tatataagag cggggatgac catgccgccc
      841 accgcggccg cgatgggcag ggcggcgctg cgccgtgagg cgagatggcc cacggtgaat
      901 tcgcgcttga tctccaaacc cacgaccaga aagaacaccg tgagcagcgc atcgttcacc
      961 caatgcacca gtggcatgct gaacgccgcg tcgccgaagc cgagggacag cggcgtctcc
     1021 cagaatcctg tgaacgcggg acccgcatcc gtgttggtaa ggacgacggc caacagcgtg
     1081 gccagcagta gcagcaggcc agtggacggc gcccaactcg cgaagcccaa agctgcgacg
     1141 cgcacacgat gcccgagtga acccagcatc gcatcggtga aggacgtctc atcccacggc
     1201 ccatcgtagc ggcggccgtt gatgaagaac gtgggcgtga agcgcacgcc actcgcgtgc
     1261 gagctggcca cgtcggcatc cacgcgttcg cgggcgcgcg tctcgagtgc ggtcgcgacc
     1321 tcgagcgggt tctcgccgag ccgcaggtcg tcggcaaccg cgcgcaggtc atcttccgtg
     1381 agcgtgcggg agcgggtcat cagcgaaacg tgcgcgctcc agaaatcatc gggcgtttgc
     1441 gcctgttcca cgagttccgc tgcccggcgg gccagctcgc tacccgagat cggacggtgc
     1501 cggaatacat atcgaacacg atcgccgagt tgcgcgcgga cttcgccaat tcgtgcattg
     1561 gcagcccggc agtgcggaca gtcataactg ccgaactcga ccagagtgat ctcggcgcgc
     1621 gcggcaccca gcgtgtggtc tcgttccgga tcaacggcgc gttccaggcg attgttcatg
     1681 actgcaatta gaacacgtag gatcccagtg ctccccaggg gtggctatcg catggcgcac
     1741 caatgacccc ctgacgcgtc tgcccgccgt ggccatcggc gtcacggcgg cgttcgagct
     1801 ctaatacgaa gggcaccacg tggcgtgcgc aaacggcggg caggccggca tcgacatgtt
     1861 cggtgccgcc tgcgatggca acctgccgtt ttccacggtc atcaccgatc tgggaatgcc
     1921 gcatgtggat ggccgccagg tcgcggcggc aatcaaggca atcgagcgga cggcaagaat
     1981 catcatgctg accggttgga ggcagttgct ggccgacggt agtgacgcac ccgcccacgt
     2041 cgacaagatc ctgagcaagc cgccaaat
//
LOCUS       DASQJS010000282         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2369347, whole genome shotgun sequence.
ACCESSION   DASQJS010000282 DASQJS010000000
VERSION     DASQJS010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2369347"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VEW08_11235"
     CDS             <1..336
                     /locus_tag="VEW08_11235"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ41357.1"
                     /translation="PADWTAPQVTLTPTLEIPQLLASMSGDAEIEEDVELPASIWLGE
                     QDSITQLPEQGVDLRAHLMVIERSLISQALQRSGGTVAHAARLLSLRRTTLVEKLRKL
                     GMAQAPEHA"
     gene            500..1480
                     /locus_tag="VEW08_11240"
     CDS             500..1480
                     /locus_tag="VEW08_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYJ41358.1"
                     /translation="MSTEMKLSDALEPVAVDSSSLLTMALARRVAVTDCSVLIVGESG
                     TGKEVLARLIHRASPRAKNNFVAVNCAAIPENMLEAMLFGYEKGSFTGAVNAHAGKFE
                     QAQGGTILLDEITEMPLALQAKLLRVLQEREVERLGGNTPVKLDVRVIATTNRLLRDE
                     VTAGRFREDLYYRLNVFPLALTPLRERRDDILPLASHLLQQRARAGQRIPALSADVAH
                     LLLTYSWPGNVRELDNLLQRAVVLTEGTVIEAEHILFEKQATRAESAANSSPVLQQTL
                     QRAERDVILEALRTAHGCRREVAEKLGISPRTLRYKLARIRNTGVDVPAA"
     gene            1503..1850
                     /gene="fliE"
                     /locus_tag="VEW08_11245"
     CDS             1503..1850
                     /gene="fliE"
                     /locus_tag="VEW08_11245"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HYJ41359.1"
                     /translation="MSNMEIDRVLAQIRSISHAGSAGGVGGLRGPSAAVGGPGAVDKG
                     FATLLKQGLDSVNQVQNKATTLATQFERGVPGVELPQVMLEMQKANVSFRALAEVRNK
                     FVDAYREIMNMPL"
     gene            1871..>2785
                     /gene="fliF"
                     /locus_tag="VEW08_11250"
     CDS             1871..>2785
                     /gene="fliF"
                     /locus_tag="VEW08_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HYJ41360.1"
                     /translation="MAQELVNSGSERVISGMKPLLLLVGVAAAIAAGVGVMLWSVGPT
                     YSLLFSNLAAEDTAQITQALESSGIPYKLENGVGAISVPAEQLAAARLKLAAQGLPES
                     GGGVNAMTRDPGFGVSAFMENARYQHALETELARTIASLQNVQGARVHLAMARQSAFV
                     SERRPGSASVFLQIKAGRRLDDENVQAIANLVASSIPELQASQVTVVDQTGRLLSAPE
                     TDSDTAMRDKMFEFSHRLEESYSNRIQEMLTPLVGPGRVRAQVVAQVDMTTTESTREQ
                     FNPQSQVVRSESTAEEAARNGAGNAGGVP"
BASE COUNT          518 a          923 c          887 g          457 t
ORIGIN      
        1 cccgcggact ggaccgctcc gcaggtcacg ctcacgccga cgctggaaat cccgcagttg
       61 ctggcctcga tgtccggcga cgccgagatc gaagaggatg tggagttgcc ggcgtccatc
      121 tggctcggcg agcaggactc catcacgcaa ctgcccgagc agggcgtcga tctgcgtgcg
      181 cacctcatgg tcatcgaacg cagtctcatc agccaggcgc tgcagcgctc cggcggcacg
      241 gtcgcgcacg cggctcgctt gctgtcgctt cgccgtacta ctttggtgga gaaacttcgc
      301 aagctcggca tggcccaggc gcccgaacac gcctgagttt cgtcgccgcg tacgccacgt
      361 gcgccggcac cttccttttt ccgcgacgga ttcatgacac tgatgtgaac ggtgtcacgc
      421 cggtacagcc ctttctggcc ggcacagctc ttgcaactgc aatcgttggc cgcccacggc
      481 caatgaactt gaggttctga tgagcaccga aatgaaactg agcgacgcgc tggaaccagt
      541 cgcggtcgat tcatcctcgc tactgaccat ggcgctcgcg cgccgggtgg cagtcaccga
      601 ttgctcggtg ctgatcgttg gcgaatccgg caccggcaag gaagtgctgg cgcgcctcat
      661 tcatcgcgcc agtccacggg cgaagaacaa cttcgtggcg gtgaactgcg cggccattcc
      721 cgagaacatg ctcgaagcca tgttgttcgg ctacgagaag ggatcgttca ccggcgccgt
      781 caacgcacac gccggaaaat tcgagcaggc gcagggcggc accatcctgc tcgacgagat
      841 caccgaaatg cccctggcgc tgcaggccaa actgctgcgc gtgttgcagg agcgtgaagt
      901 cgaacgcctc ggcggcaaca caccggtgaa gctcgacgtg cgtgtcatcg ccacgaccaa
      961 ccgcctgctg cgcgacgaag tcaccgctgg ccgtttccgc gaagacctct attaccgcct
     1021 caacgtattt ccgctggcgc tgacgccgct gcgtgaacgc cgcgacgaca tcctgccgct
     1081 ggcctctcat ctattgcagc agcgcgcccg ggcagggcag cgcatcccgg cgctctccgc
     1141 cgatgtcgcg cacctgctgc tcacctacag ctggcccggc aacgtgcgcg agctggacaa
     1201 cctcctgcag cgtgccgtcg tgctgaccga aggcacggtc atcgaagccg aacacatcct
     1261 gttcgagaag caggcgacgc gagccgaaag cgccgccaac tcgtcgccgg tccttcagca
     1321 gacgctgcag cgtgccgaac gcgacgtgat tctcgaagcg ctgcgcacgg cgcatggctg
     1381 ccggcgcgaa gtggcggaga agttaggcat cagcccgcgc accttgcgct acaagctggc
     1441 gcgcatccgc aataccggcg tcgatgttcc ggcggcgtaa tcggtaactc cggagaaatc
     1501 ccatgagcaa tatggaaatc gaccgcgtgc tggcgcagat ccgcagcatc tcgcatgccg
     1561 gcagcgccgg tggcgtcggc gggcttcgcg gcccttcggc cgcagtggga ggcccgggtg
     1621 cagtcgacaa gggcttcgcg accttgctga agcaggggct cgactcggtc aaccaggtgc
     1681 agaacaaggc caccacgctc gcgacgcagt tcgagcgcgg agtgcccggc gtcgaattgc
     1741 cgcaggtcat gctggaaatg cagaaggcca acgtgtcatt ccgcgctctc gccgaagtac
     1801 gcaacaagtt cgtggatgcg taccgcgaga tcatgaacat gccgctttga gcggctaaag
     1861 gaatctttcg atggcgcagg aactggtcaa ttcaggctcg gagcgagtca tcagcggcat
     1921 gaagccgctg ctgttgttag tcggagtcgc agcggcgatc gccgccggcg tgggtgtgat
     1981 gctctggtcc gtgggcccca cgtacagcct cctgttctcg aacctcgcgg ccgaagatac
     2041 cgcgcagatc acccaggcgc tggaaagctc cggtatcccc tacaagctcg agaacggcgt
     2101 gggcgccatc agcgtgcccg ccgagcagct cgccgcggcg cggctcaagc tcgccgcgca
     2161 aggattgccc gagagcggcg gcggcgtgaa tgccatgacg cgcgatccgg gtttcggcgt
     2221 cagcgcgttc atggagaacg cacgttacca gcacgcgctc gaaaccgagc tcgcccgcac
     2281 catcgccagc ctgcagaacg tgcagggtgc gcgcgtgcat ctcgccatgg cgaggcagtc
     2341 cgcgttcgtg agcgaacgcc ggccgggctc cgcgtcggtg ttcctgcaga tcaaggcagg
     2401 ccgtcgcctc gacgacgaaa atgtgcaggc cattgcgaat ctggtggcct ccagcattcc
     2461 cgagctccag gcatcccagg tgaccgtggt cgaccagacc ggccggctcc tgtccgcacc
     2521 ggaaaccgac agcgacaccg cgatgcgcga caagatgttc gagttctcgc atcgcctcga
     2581 ggaaagttat tcgaatcgca tccaggagat gctgactccg ctcgtgggcc ctggccgggt
     2641 gcgggcgcag gtggtcgcgc aggtggacat gaccaccacc gaatccacgc gcgaacagtt
     2701 caatccgcag agccaggtcg tgcgcagcga atcgacggcc gaagaagccg cacgcaacgg
     2761 tgccggcaat gccggtggcg tgccc
//
LOCUS       DASQJS010000283        18177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3941784, whole genome shotgun sequence.
ACCESSION   DASQJS010000283 DASQJS010000000
VERSION     DASQJS010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18177
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3941784"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VEW08_11255"
     CDS             complement(<1..869)
                     /locus_tag="VEW08_11255"
                     /inference="COORDINATES: protein motif:HMM:NF020068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HYJ41361.1"
                     /translation="MSGVTPADGVELTTSAKSAPQAAPAATLTTVIIDGSSVYSPPQL
                     FASYRDQLGRPYSRESTRAIASALADLYVRDGYVKPEFTLDDALTSRGVLRLQVYEAQ
                     VTNVVFEGDGGKFNDALDGIGAQLENARPLRKDDVAQALRSMRQIAGLAVTATTRRDP
                     DIRNAFELVVQADFSPIDGVVRMNNRGTDQVGPAFMLGQVFANGLLGRQEKIGLIFAA
                     ATDHDEYLGGGLYVDTALGAGGARGNALLFRSHSAPNEAPDNLDDEYTRERFTLRFSK
                     PFQQRSELSLTGSL"
     gene            1093..2763
                     /locus_tag="VEW08_11260"
     CDS             1093..2763
                     /locus_tag="VEW08_11260"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HYJ41362.1"
                     /translation="MSCDNKKHSAMFHVVRLTATLLFACAALLGAPAAFAVDSGDIIV
                     VSSKGEVHITMNGAERKVRAGGVLELPAQVRTGRDGNVELRQGTTTVSVGPETLLEFP
                     ALEKRGAPIDRIVQPRGNAFYNIGKRDGRKLRVDTPYLVGVIKGTQFNVASQDEATTI
                     SLFEGLLEVRATDDSDVVDLKAGEIASRKRGDKSISIIRMDAGKAPPTSSRPSPGNGS
                     NSGAPRPTTPRVPPANPGDEPLVADHRPIDVRPDVGLNVDARTGGSSVDTGVAATVGV
                     GQGLDATTSVGVNAASSGVNVGADTSVGLGNTVDVAANAAVNVGPAGADVVTNIGVDA
                     GPVTADVGVTAAVDAAAGTVDVSTGVGLDAGPVTADLGVTAAVDAAAGTVDVSTGVGV
                     DTGPVTADLGASTSVDLGAGTIDTSVSTSVDAGPLTTDVAAGAAVDLTSVAVDTAVDV
                     GVVTPVADVDLGATAAVDLTSPTVDTAVDVGVVTPVVDVDLGASAVVDVGAGAIDLGV
                     DVAGADIDLGVSVADTVDVVVPVVETVTDTVVDVGGLLDGLLRRPGRR"
     gene            2760..5573
                     /locus_tag="VEW08_11265"
     CDS             2760..5573
                     /locus_tag="VEW08_11265"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ41363.1"
                     /translation="MIRFGRRYGWWLTALAALILVGGLRATGQLQSLENAAADTRARL
                     MMHEVRSDIVIVGIDAASLAELNEWPWPRRHHAKLVEQLTHAAPSSVFLDIDFSSQTR
                     SLDDALLEAALAKPRDFPLVLPTHFQNATGADSDLLISRPLRRFTRRTELAVVNVLAD
                     RDGLTRRWRNIWTVDGARMHSVIDPHRVLPDERDVLIDYSISPSSFTYVSYVDVLEGR
                     VQRAVLADKTIFVGATAVELNDMLAVPVHRSLPGIVVQALATESVSQGALRPSSTWMW
                     VALLAFWIALVSVMYGAKWQRNLAVLVLALASIAGIALFAYAYKRLILDSAAPMLAVA
                     LLFVAATVRSLEAQTWRALSYALGMRRRDALLKSVVQSSTDCIVCIDEAGIIKTANPA
                     ASRLFGCAIYELIDEPIAKFITLLAGDGAGARLGALHGEIRECDARTLTGEVFPVEIS
                     VSRVRLNSERLYTAIVRDIRERRAQQRRLQHQATHDSLTGLPNRAALLAHLETALGLQ
                     PVRPALALLMLDLCRFKEVNDTLGHNVGDWVLCNVAQRFAAALGDRGFIARIGGDEFT
                     VVLAAPEREVVATTAQLLADCLREPIDVAGISIEVGVSIGVAHYPDDAGDAHTLLRHA
                     DVAMYVSKRRGATFEYYDAAHDENTVRKLAIGGELRAAIASNALELHFQPQVNLRSGM
                     VESAEALLRWFHPTQGAIGPDEFIAIAEATDLIRPLTEWTLNGALAQVRAWRDRGVHV
                     RIAVNLSARLLQDTGFPAQLRKLLEESGVSAASLELEITESAMMLDPARALRVIQEIA
                     RLGVLISVDDFGTGYSSLGYLRDLPVNSLKLDKSFVMGMRNNTDDRVIVESTAQMAHA
                     LRLELVAEGVETEWDARFLAAAGYDYAQGYHFSRPLPADKCLAWIVEFNATAMLTLGD
                     ANVTTALRSRTECEHEDFESRTA"
     gene            complement(5584..6702)
                     /locus_tag="VEW08_11270"
     CDS             complement(5584..6702)
                     /locus_tag="VEW08_11270"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HYJ41364.1"
                     /translation="MKSNLAMVAAWFCLAVAMGQTIPAQAEEPGRFYGMLRSRDLTPF
                     GFLRLDMRPAHAVSIEKHTFAFEAELGYQNTWALSENTEKYLKSLEGSGRRTIGPAEI
                     QAIRDLPGENYLFDLESATLDMAVHYKISSQWTAYAFATAVSYQGGFLDSTIEGFHKA
                     LGLNSYGRLAVERNGSHLIYDLKQTKGVLSNAPKSPGFLDPTFGLRYGGISLPGPWQM
                     SVETAVKIPYDGERPLLSTGHTDYGMQVSVRRLGERNAIHMDFAAVYYAGESMPSEHD
                     SQVIPTIVIGWERQLSARTNLNLQGYASKSVYKPEQTDLHELLADKFQMSLGVRHRFD
                     CCVASFGITENLQNLNNTPDIGFQMGFAWVPRLKPQQR"
     gene            complement(7195..7575)
                     /locus_tag="VEW08_11275"
     CDS             complement(7195..7575)
                     /locus_tag="VEW08_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HYJ41365.1"
                     /translation="MMKFANDSEAFLSVLTLVAGADQSGSLEERDFLFNKVKGLAIFG
                     SPSTADFSKLLGKVTDAVYSGVAQQDGEFTAAGIESLLGEVKKTLSAELRKTLVQVAV
                     ELGGSDGADAKETALLGQIRRALG"
     gene            complement(7665..8009)
                     /locus_tag="VEW08_11280"
     CDS             complement(7665..8009)
                     /locus_tag="VEW08_11280"
                     /inference="COORDINATES: protein motif:HMM:NF008107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HYJ41366.1"
                     /translation="MALTIYGIRNCDTMKKARAWLEAHDVAYEFHDYKLAGIDRTKLE
                     TWVKTVGWEVLLNRAGTTFRKLPESAKAGLTEARAIKLMLEQPSMIKRPVLERGRTLL
                     VGFSEARYSAIR"
     gene            complement(8013..9290)
                     /locus_tag="VEW08_11285"
     CDS             complement(8013..9290)
                     /locus_tag="VEW08_11285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ41367.1"
                     /translation="MTELERSTMARVTLRLLPFLLLLYIISWLDRVNVGFAKLQMNSD
                     LGMSDTAYGIGAGIFFLAYAACEIPSNLALVRFGARRWIARIMITWGLISAGMMFVQG
                     EISFCVMRLLLGAAEAGFLPGIVYYLSQWFPRPQRAKAVSWFMIGIPLSIVFGGPLSG
                     WLLGFEGHLGLHGWQWMFLLEGLPAVALGFVVLGYLTDKPSDAQWLAPEQREWLTRTI
                     EDEHTVAQARQKVSVADALKHPTVWLLAGIMFCCQTGSYGLTLWVPTIVKGLSGFTNL
                     EVGFISALPYIAAAAGMVLIGRSSDRSGERFMHVAIPTAIGAVGFVATALLKSPIPAM
                     IALTVAAVGDYGTRGPFWALPGKFLTGSSAAAGIALINAMGAVGGFIGPVAVGILKDT
                     SGNFESGLFLLAVILLAGSILTLVLRHSPALKD"
     gene            9347..10438
                     /locus_tag="VEW08_11290"
     CDS             9347..10438
                     /locus_tag="VEW08_11290"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYJ41368.1"
                     /translation="MSEPAGEWIDVGAAEDFKRSPLREAKLGERLVAVSFRDGKFGVI
                     SNVCNHVGGPLGKGRLDGEFVVCPWHQYKFHRCTGEGEPGYEADKVPGYGVKEENGRL
                     LVTAKPTSKRGHLPHEPHPLTRSTDRAPGGLRVGGISTTAMDAKNPRFSTSEHLLNVA
                     LEHASTALGAETKLIRLNDLNFRNCEGYYSKAAKACTWPCSVTSMDKTDQLDQVYEMA
                     VHWADILLIATPIRWGAASSLYYKMVERMNCIQNQITLNNKVLIRNKVAAFIITGGQD
                     GIQAVAGQMMGFFSEIGYLLPQFPFIAHSLGWEFEEMERNVDFVQASEGLRAGARDLV
                     ARASEMSKCLLEREIARSTIERGGRKASA"
     gene            complement(10446..11456)
                     /locus_tag="VEW08_11295"
     CDS             complement(10446..11456)
                     /locus_tag="VEW08_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41369.1"
                     /translation="MRTAVIALALATAAFAASTVYFARELQVERANAGAEPPIYSTSS
                     VVAAPGAAIEPAPSPTSAPSPTSEPTTITFATLSGPGAMASTEPVPGSTYALIQAEQR
                     KYQEQLIKRLDDPEQRAEMLEEFRTMARNSNPRLAQTLKMSGEEAERLIELLAQQQLD
                     QQEKFARCSVDSSCDFQRIGREFAGANERELANLLGPEGRQKFEQYRSTMGERTSVTE
                     LRTRLSDADFLPEEQAEALIVVLADERSRISLDASKRGGGLMHFGSGTGTIIMAGESP
                     EAQLESARENSQRMRTRAAEVLTPAQLRVFEEMQEELLRSTRQQLRQMRQFNAVTVTT
                     PN"
     gene            complement(11490..12170)
                     /locus_tag="VEW08_11300"
     CDS             complement(11490..12170)
                     /locus_tag="VEW08_11300"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HYJ41370.1"
                     /translation="MRPRIVILSGSGLSAESGLPTFRDAAGLWRQHSWQELASPEGWR
                     RNPALVLEFYNERRAKAWAAQPNSAHVAIAALETKFDVVVVTQNVDALHERAGSTNVL
                     HVHGELAFARGTGPARRRYRIDGAPIEIGQLCDEGTQLRPDIVWFGEMTQFRDEAREH
                     VAAADKILVVGTSLSVYPAASLVDDARADADKVLNSLEMDEVPRGFVFRPGPATRVVP
                     RIIKEWIS"
     gene            complement(12189..12635)
                     /locus_tag="VEW08_11305"
     CDS             complement(12189..12635)
                     /locus_tag="VEW08_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41371.1"
                     /translation="MEMTKVFRGLVASSLGLFALSIGISFLEPDFPDAVNEYLDGDGA
                     GPLLSGLFSGPLSLQILLGALVLGFLAAYLASCVGLLLFRRWARATFIGVAVAGIVVI
                     PWEGVSLTTPLMSLVETLFSMIDGAILVMLLFDPIKAKFAKSSSPG"
     gene            complement(12650..13648)
                     /locus_tag="VEW08_11310"
     CDS             complement(12650..13648)
                     /locus_tag="VEW08_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41372.1"
                     /translation="MSKLVAGLAVSTAVCAAAAVYFRQQLVMERENSAHLASELAIAS
                     APRAPAPKVVATPSAPAAPIAPATSVTEPAQKAAVTKVSALESREAPRIMTDAQKSAM
                     RAQATDFLGKYDSPGGRARLRDQQIERTRALLEGFDKDRNLAPGAWQKLIEEVADTEL
                     EKRAVISRCALDPACVTPALSELLASRKQALQELLGDEGLDEMKNWAMTEPARRSVKG
                     LSARLPSTAPLSSAQSSALVAALTAEREAAMRDFANEGTHIKGFSNVDRMSLFYADAL
                     PAEGRMASAEAYVQRTRARAATVLAGEQLITFNQLQDDLLHDFRRMESRRTAEQKP"
     gene            complement(14101..15120)
                     /locus_tag="VEW08_11315"
     CDS             complement(14101..15120)
                     /locus_tag="VEW08_11315"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HYJ41373.1"
                     /translation="MLARVVENVYWLSRYLERAENTARIIGVNTNLLLDLPGGIAPGW
                     LPLVDISGNRAEFDARETKGEERDVVNFLIADKQNPGSICSSLAAARENARTLREILP
                     TEAWELLNEFFAEFNKDLSAGINKRTRFDFLKRVVVTLQTLSGMLDGTMNRNDAHTFS
                     TLGRNLERADMTSRIVDVRSAQLLPAETPELRPFETVQWMSVLKSLSGYQMYRLKMRT
                     RVKRTDVLQFLLRDDQFPRSCQFCLMQLESSLTPLPRSESVLEVLEGAAKFIQRAPLA
                     TLDQPGLHELIDNIQLHISNVHSRLAEIYFPSGDISGVQRMTGQKQSQSQRQTSLSFG
                     ERAKS"
     gene            complement(15208..16614)
                     /locus_tag="VEW08_11320"
     CDS             complement(15208..16614)
                     /locus_tag="VEW08_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010563769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HYJ41374.1"
                     /translation="MGPGGGARAAGRQLMRYLTDMDGDEIAVRQQAAELAIKAMGITF
                     TVYHEQDGSIDRAWPLDIIPRTISRKEWQKIEAGLIQRAAALNLFIDDVYHGQKFIKD
                     GLMPSSIIATSKNFRANVRGIKPPLGTWAHICGSDLIRDKDGTVYVLEDNLRVPSGVS
                     YMLENRQVVKRVFPELFESAAILPVDDYPSQLFDMLTALSPRKETRPEVVVLTPGIYN
                     SAYFEHAYLARQMGCELVEGRDLAVDSDDCVYMQTVRGWTRVDVIYRRIDDDFLDQEV
                     FRKDSSLGVPGLMRAWAAGKVALANAPGAGVADDKLVYTYVPDMIKYYLGEDAILPNV
                     PSYRCGEARQLDHVLANLDQLVVKPVDGSGGYGMLMGPMSTKAERAKFADSLKADPRS
                     FIAQPIVTLSTVPTLVNNRLEPRHVDLRPFILSGPQTAVTTGGLTRVALRKGSLVVNS
                     SQGGGSKDTWIVDTEGAG"
     gene            16825..>18177
                     /locus_tag="VEW08_11325"
     CDS             16825..>18177
                     /locus_tag="VEW08_11325"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HYJ41375.1"
                     /translation="MSIHVALNHVTHYRYDRLVALGPQVVRLRPAPHCRTRIISYSLK
                     VTPAKHFINWQQDPEANYLARYVFPEKANELRIEVDLVAEMAVLNPFDFFLEPYAETF
                     PFAYQGAEKRDLAPYLVMFRGEPKFKAYLGTIPRDRKRTIDFLVELNAKLANDIKYLI
                     RMEPGVQTPELTLTKCSGSCRDSAWLLVQLLRHMGLAARFVSGYLIQLTADMKSLDGP
                     PGPEKDFTDLHAWCEVYLPGAGWIGLDPTSGLLAGEGHIPLACTPEPGAAAPVTGAVD
                     KAEVEFEHHMKVERIWEAPRVTKPYTETQWKEIESLGHAIDAQLLAGDVRLTMGGEPT
                     FVSVDDPDGAEWNTTALGPDKRRLAVEVYNRLKQKYAPQGLSHFGQGKWYPGEPLPRW
                     SLNCFWRKDGQPIWQNPELYADESIKRDIDATVAQRFLATVAMSLGVSPAFVFAAYED
                     T"
BASE COUNT         3248 a         5711 c         5874 g         3344 t
ORIGIN      
        1 aggcttccgg tcagcgacag ttctgaacgc tgctggaatg gctttgagaa acgaagcgtg
       61 aagcgctcgc gggtgtactc atcgtcgaga ttgtcgggcg cttcgttggg cgccgaatgc
      121 gaacggaaga gcagggcgtt gccgcgcgcg ccgccggcgc cgagcgcggt gtcgacatag
      181 aggccgccgc ccaggtactc gtcatgatcg gtggcggccg caaagatgag gccgatcttt
      241 tcctgccgtc ccagcaatcc gttggcaaac acctggccga gcatgaatgc cggacccacc
      301 tggtccgtgc cgcggttgtt catgcgcacc acgccatcga tcggcgagaa gtccgcctgc
      361 accacgagct cgaatgcatt gcgaatgtcc gggtcgcgtc gcgtggtcgc cgtgacggca
      421 aggcccgcga tctgccgcat ggaccgcagc gcctgcgcca cgtcatcctt gcgcagcggg
      481 cgggcgtttt cgagctgcgc gccgatgcca tccagcgcgt cgttgaattt tccaccgtcg
      541 ccctcgaaga ccacgttggt gacctgcgct tcatacacct gtaatcgcaa tacgccgcgc
      601 gaagtcagcg cgtcgtcgag cgtgaattcc ggtttcacgt atccgtcgcg cacatagagg
      661 tccgcgagcg cactggcgat ggcgcgcgtt gactcgcgcg aatacgggcg gccgagctgg
      721 tcgcggtacg aggcgaacag ttgtggtggt gaatacacgc tcgagccgtc gatgatgacc
      781 gtggtgagcg tggcggcggg cgcagcctgc ggtgcggatt tcgcggaagt agttagctcg
      841 acgccgtcgg ccggggtgac gccggacaag gccagcgcca ttatgacagc cgcacaaacc
      901 cgtggtccgg cgaagcgcgg gaagcgtgtc tcgacgccac ttattgtcag ctgtccgtac
      961 atatctgaaa gcccgcgggg gaatggcacg tattctcccg aaagtacgcg cgcgacgatg
     1021 cgatgcgtct cacattgttt cagggctttg cttagtacga tgcgccggaa acgatgaagg
     1081 ggatttcagg ttatgtcgtg cgacaacaag aagcattccg ccatgttcca tgtagttaga
     1141 ttgaccgcca cgctgctgtt cgcatgcgcg gcgctgctcg gcgcccccgc ggcgttcgcc
     1201 gtcgattccg gcgacatcat cgtcgtcagt tcgaagggcg aggtgcacat caccatgaac
     1261 ggagccgagc gcaaggtgcg cgccggcggc gtgctcgagc tgcccgcaca ggttcgcacg
     1321 ggccgcgacg gcaacgtcga gctgcgccag ggaaccacca ccgtcagtgt cggccccgaa
     1381 accctgctcg aattcccggc gctcgagaaa cgcggcgcgc ccatcgatcg catcgtgcag
     1441 ccgcgcggca acgcgttcta caacatcggc aaacgcgacg gacgcaagct gcgcgtcgat
     1501 accccatacc tggtgggagt catcaagggc acgcagttca acgtcgcgtc gcaggacgag
     1561 gccaccacga tttccctttt cgaaggactg ctcgaagtcc gcgccaccga cgacagcgac
     1621 gtcgtcgatc tgaaggccgg cgagatcgcg tcgcgcaagc gcggcgacaa gtccatcagc
     1681 atcatcagga tggatgccgg caaagcgccg cccaccagtt cgcgcccgtc cccgggcaac
     1741 ggttcgaata gcggggcgcc gcgccccacg accccacgtg tcccgccagc caacccaggg
     1801 gatgaaccgc tggtggcaga tcaccgcccg atcgatgtac gtccggacgt cggcctcaat
     1861 gtcgacgcgc gcaccggcgg ctcgtcggtc gacacgggcg tcgccgccac cgtgggcgtg
     1921 ggacagggcc tcgacgcgac cacgagcgtg ggggtgaacg ccgccagttc gggagtcaat
     1981 gtcggcgcgg acacctccgt cggcctgggc aacaccgtcg atgtggccgc aaacgcggct
     2041 gtcaatgtcg gcccagccgg cgctgacgtg gttaccaaca ttggcgtcga tgccgggcct
     2101 gtaactgctg acgtcggcgt caccgccgcg gtggacgcgg cggccggaac ggtggacgtc
     2161 tccacgggtg tcggcctgga tgcaggccct gtcaccgctg acctcggcgt gaccgccgcg
     2221 gtggacgctg ccgccgggac cgtagatgtc agcacgggtg tcggcgtaga tacaggccca
     2281 gtcaccgccg acctcggcgc cagcaccagc gtcgatctcg gcgccggcac gatcgatacc
     2341 tccgtgagca ccagcgtcga tgctgggccg ctaactactg acgtggcggc cggtgccgcc
     2401 gtggatctga cttcggtcgc cgtcgatacc gccgtcgacg tgggcgtcgt cacgccggtg
     2461 gccgacgtgg acctcggcgc cactgccgcc gtggatctga cttcccccac cgtcgatacc
     2521 gccgtcgacg tgggcgtcgt cacgccggtg gtcgacgtgg accttggcgc cagtgccgtc
     2581 gtcgacgtgg gcgccggggc catcgacctg ggcgtggacg tcgcgggcgc cgatatcgat
     2641 ctcggcgtca gcgttgcaga caccgtcgat gtggtcgtgc cggtcgtcga aaccgtgacg
     2701 gacaccgtcg ttgacgtcgg gggacttctg gacggcctcc ttcgccggcc aggtaggaga
     2761 tgatccgctt cggtcgtcgt tatggctggt ggctgactgc actcgccgca ttgatcctgg
     2821 tcggcgggct acgcgccact ggccagctgc agtcgctcga gaatgcggcg gctgacacgc
     2881 gcgcccgatt gatgatgcac gaggtgcgct ccgacatcgt catcgtcggc atcgacgcgg
     2941 ccagcctggc ggaactgaat gagtggccgt ggccgcggcg ccatcacgca aaactggtgg
     3001 aacagctcac gcacgccgca ccgtccagcg ttttcctcga catcgatttc agttcgcaga
     3061 cccgatccct cgacgatgcg ctgctcgaag cggcgctcgc gaaaccgcgg gatttcccct
     3121 tggtgctgcc gacgcatttc cagaacgcca ccggcgctga ttccgacttg ctcatcagcc
     3181 ggccattgcg ccggttcacg cgccgcaccg agctggcggt ggtgaatgta ctggccgatc
     3241 gcgatgggct cacgcgccgc tggcgaaaca tctggacggt ggatggtgcg cgcatgcaca
     3301 gcgtgatcga tccgcatcgc gtgttgccgg acgagcggga tgtgctcatc gactattcga
     3361 tttcgccgtc ttctttcacg tacgtatcct atgtcgatgt gctggaaggc cgcgtgcagc
     3421 gcgcggtgct cgccgacaag accatcttcg tgggtgcgac cgcggtcgaa ctcaacgaca
     3481 tgctcgcggt gcccgtgcat cgctcactgc ccggcatcgt ggtgcaggcg ctggccacgg
     3541 agagcgtgag ccagggagcg cttcgtccct cctcgacgtg gatgtgggtc gcgctgttgg
     3601 cgttctggat cgccctggtg tcggtgatgt acggcgccaa gtggcagcgc aacctggcgg
     3661 tgctggtatt ggcgctggcg tccatcgccg gaattgcgct gttcgcgtat gcgtacaagc
     3721 gtctgatcct cgattccgcc gcgcccatgc ttgccgttgc gctgcttttc gtcgccgcca
     3781 ccgtccgctc gctcgaggca cagacctggc gtgcgttgtc gtatgcgctg ggcatgcgcc
     3841 gtcgcgacgc gttgctgaag agcgtggtgc agtcgtcgac ggactgcatc gtctgcatcg
     3901 acgaggccgg catcatcaag acggccaacc ccgcggcgtc gcgcctgttc ggttgcgcca
     3961 tttatgagct gatcgatgaa cccatcgcca agttcatcac gctgctggcg ggcgatggcg
     4021 ccggcgcccg gctcggcgcg ctgcatggtg agatccggga gtgcgacgcg cgcacactga
     4081 ccggcgaagt gttcccggtg gagatttccg tgagccgggt gcggctcaat tccgaaaggc
     4141 tgtacaccgc catcgtgcgc gacattcgcg aacggcgcgc gcagcagcgc cgccttcagc
     4201 accaggcgac ccacgattct ctcaccgggc tgccgaatcg cgccgcgctg ctcgcgcacc
     4261 tcgagaccgc gctcggcttg cagccagtgc gtccggcgct ggcgctgctc atgctcgacc
     4321 tgtgccgctt caaggaagtc aacgacaccc tcggccacaa cgtcggcgac tgggtgctgt
     4381 gcaacgtggc gcagcgtttc gcggcagccc tcggcgatcg cggtttcatc gcgcgcatcg
     4441 gtggggacga attcacggtg gtcctggccg cgcccgagcg cgaggtcgtc gcaaccaccg
     4501 cgcagctgct cgcggattgc ctgcgcgagc ccatcgacgt cgcgggaatc tcgatcgagg
     4561 taggtgtgag catcggcgtt gcccactacc cggacgatgc gggcgacgcg cacaccctgt
     4621 tgcgccacgc ggacgtggcc atgtacgtgt ccaagcggcg gggcgccacg ttcgagtact
     4681 acgacgcggc gcacgacgaa aacaccgtgc gcaagctcgc gatcggtggc gagctgcgtg
     4741 cggccatcgc cagcaatgcg ctcgagctgc acttccagcc gcaggtgaat ctgcgcagcg
     4801 ggatggtcga gagcgccgaa gcgttgctgc gctggtttca tcccacgcag ggtgcgatcg
     4861 ggcccgacga attcatcgcc attgccgagg ccaccgacct gattcgtcca ctgaccgagt
     4921 ggaccctgaa cggtgcgctg gcccaggtcc gtgcctggcg cgatcgcggg gtacacgtgc
     4981 gcatcgcggt gaacctctct gcgcggctgt tgcaggacac gggttttccg gcgcaactga
     5041 gaaaactgct cgaggagtcc ggcgtgtctg ctgcctcact cgaactcgag atcaccgaga
     5101 gcgcgatgat gctcgacccg gcgcgtgcgc tgcgtgtgat ccaggaaatc gcccgcctcg
     5161 gggtgctcat ttccgtcgac gatttcggca ccggatattc atcgctcggc tacctgcgcg
     5221 acctgccggt gaactcgctc aagctcgaca agtctttcgt catgggcatg cgcaacaaca
     5281 ccgatgatcg cgtgatcgtg gaatccaccg cgcagatggc gcatgcactc aggctggagc
     5341 tggtggctga aggcgtggaa accgaatggg acgcccggtt tctggccgcg gccggctacg
     5401 actacgcgca gggctatcat ttcagccgtc cattgccggc cgacaaatgc ctggcctgga
     5461 tcgtggaatt caacgccacg gccatgctca cgctcggcga cgccaacgtg accacggcgt
     5521 tgcgttcgcg cacggaatgc gagcacgagg atttcgaatc tcgcaccgcc tgaattgggg
     5581 ccactaccgc tgttgtggct tcagccgcgg cacccacgcg aatcccatct ggaatccgat
     5641 gtcgggcgtg ttgttgaggt tctgaaggtt ctccgtgatg ccgaacgatg cgacacagca
     5701 atcgaagcga tgtcgcacgc caaggctcat ctggaacttg tcagcgagca gttcgtgcaa
     5761 gtccgtctgt tcgggcttgt agacactctt gctcgcgtag ccctgcagat tcaggttggt
     5821 gcgcgcggac aactgccgtt cccagccaat cacgatagtc ggaatcactt gtgaatcgtg
     5881 ttcggagggc atcgattcgc cagcgtagta cacggcggcg aagtccatgt gaatcgcgtt
     5941 gcgttcaccg agccgtcgaa cggatacctg cattccgtag tcggtgtgcc ccgtcgacag
     6001 cagcgggcgc tcgccgtcgt agggaatttt caccgcagtc tccaccgaca tctgccatgg
     6061 cccaggtagt gagatgccgc cgtaacgcag cccgaacgtc gggtcgagga acccgggact
     6121 cttcggtgca ttcgacagca cgcccttcgt ctgcttgagg tcgtaaatga ggtgcgagcc
     6181 attccgttcg acggcgagac gaccgtagct gttgaggcct aacgccttgt ggaaaccttc
     6241 gatggtcgag tccaggaagc cgccctggta ggacacggcg gtggcgaagg cgtacgcggt
     6301 ccactggctc gagatcttgt agtgcaccgc catgtcgagc gtcgcggatt ccaggtcgaa
     6361 tagatagttc tcgccgggca gatcgcggat ggcctggatc tccgccgggc cgatcgtgcg
     6421 gcggccggaa ccctcgagcg atttgaggta cttctccgtg ttttcgctca atgcccacgt
     6481 gttctggtag ccgagctcgg cttcgaacgc gaaggtgtgc ttctcgatgc tgaccgcgtg
     6541 cgccggacgc atgtcgaggc gcagaaatcc gaagggagtc agatcgcgtg aacgcagcat
     6601 gccgtagaag cggccaggct cctcggcttg agcgggaatc gtctgtccca ttgcaacggc
     6661 gaggcagaac cacgcggcca ccatggccag attgctcttc atcaaaactc catttccctc
     6721 gaccggcaga tttaccagcc gaggcgcgat tcgacaatcg gatcgagccc cctgaaaagg
     6781 ccgggagcat tgagaaagta ccgccgggac ctgcgttgtg aaaaatccac aagagttcat
     6841 tcaggtggga gtataagggg gcccatgggt cgcagattca ccacagccgg gcttttcttt
     6901 tgcggtgctg aggcgtatct atgccgcgtc tctgggacag ctattcatca ccaggaaggt
     6961 gcgtatgaaa aagcaaatca ggaataggcc cggcaacggc cgtgacgcgg gcacctgcga
     7021 cccgccgggc accttctggg cggatgtcgc caggttcggg ttctaactag tgaagtcgag
     7081 gggggacggg agacggaaca gcgggttcag cggaatccgg cgtgagtctc ctttgaatcg
     7141 ggcgccgtcc ggatgcactc cgggcggcgc cttgttcgtt ggacgatccg cggctcagcc
     7201 gagcgcgcgg cggatctggc cgagcagcgc ggtttctttc gcatcggcgc cgtcgctgcc
     7261 accgagctcg accgcgacct gcacgagcgt cttgcgaagt tccgcgctca gcgtcttctt
     7321 cacttcaccg agcagtgact cgattccggc ggcggtgaat tcgccatcct gctgggcgac
     7381 gcccgagtag acggcatcgg tgaccttgcc aagtagtttg gaaaagtccg cggtcgaggg
     7441 gcttccgaag atggccagtc ccttgacctt gttgaatagg aaatcccgct cttcgagact
     7501 gcccgactga tcggctccgg caaccagagt cagcaccgac agaaaggctt ccgagtcatt
     7561 cgcgaatttc atcatgatca ctccctggtc atcgaaatct caacgggccg cgaaatctac
     7621 gtgatttccc ggcgtgtcgc aacgaaagtg cgcggcacgt ccggctatct aatggccgaa
     7681 taacgcgcct cgctgaagcc tacgagtagc gtccggcctc gctcgagcac cggccgcttg
     7741 atcatggacg gctgctcgag catgagcttg atggcgcgtg cctcggtcag gccggccttt
     7801 gcggattccg gaagtttgcg aaacgtggtg ccggcacgat tcagcagcac ttcccagcca
     7861 actgtcttta cccaggtttc gagcttcgtg cgatcgatgc cggcgagttt gtagtcgtgg
     7921 aattcgtagg caacgtcgtg cgcctcgagc caggcccggg ccttcttcat cgtgtcgcag
     7981 ttcctgatgc cgtagatagt cagtgccatg cctcagtcct tgagcgccgg cgagtgacgc
     8041 agtaccagcg tcaggatcga accggccagc aggattaccg cgagcaggaa cagcccgctt
     8101 tcgaaattgc ccgaagtgtc cttcaggatg ccgaccgcaa ccgggccgat gaagccaccg
     8161 actgcgccca tggcattgat caacgcgatg cccgccgcag ccgagctgcc agtcaggaat
     8221 ttgcccggca acgcccagaa cggaccgcga gtgccgtagt cgccgaccgc ggccacggtc
     8281 agcgcgatca tcgcgggtat gggcgacttg agcagggcgg tggcgacgaa gccgaccgca
     8341 ccgatcgccg tcggaatcgc gacgtgcatg aagcgttcgc cgctgcgatc ggagctgcgg
     8401 ccgatcagca ccatgcctgc cgcggcggcg atgtatggga gtgcggaaat gaagccgacc
     8461 tcgaggttgg tgaatccgga caagcccttg acgatggtcg gaacccagag cgtgaggcca
     8521 taactaccgg tctggcagca gaacatgatg ccggcgagca gccagacagt gggatgcttc
     8581 agcgcatcgg ccacgctcac cttttgccgt gcctgcgcca ccgtgtgttc atcctctatg
     8641 gtacgggtca gccattcccg ctgctcaggg gccagccact gggcgtccga aggcttgtcg
     8701 gtgagatatc ccagcactac gaagccaagc gccacggcgg gaagaccttc gagcaggaac
     8761 atccattgcc agccatgcaa cccgagatgc ccttcgaatc ctaacaacca tcccgacagc
     8821 ggcccgccga acacgatgga cagcggaatg ccgatcatga accaggacac cgctttggcg
     8881 cgttgcggcc gcggaaacca ctggctcagg tagtagacga tgcccgggag gaaacccgcc
     8941 tcggcggcgc caagcagcag ccgcatcacg cagaagctga tctcgccctg cacgaacatc
     9001 atgccggcgg agatcaagcc ccaggtgatc atgatgcgcg cgatccagcg ccgcgcgccg
     9061 aaacgcacca gggccaggtt gctggggatc tcgcaagctg cgtaggcgag gaaaaagatg
     9121 ccggcgccga tcccgtaggc cgtatcgctc atgccgagat cggagttcat ctgcagtttg
     9181 gcgaagccga cgttgactcg gtccagccac gagatgatgt acagcagcag gagaaatggc
     9241 agcaggcgca aggtgacgcg cgccatggtc gagcgttcga gttccgtcat cgtgcggagt
     9301 gtaggggata tgattgccgc cagcttcagg attgcaggag ttgagaatga gcgagccagc
     9361 gggggagtgg atcgacgtcg gcgccgccga agacttcaag cgctcgccgc tgcgcgaggc
     9421 gaagctcggc gagcggctgg tggccgtgtc gttcagggac ggcaagttcg gcgtcatctc
     9481 caatgtctgc aatcacgtcg gcggaccgct cggcaaaggc cggctcgacg gagagttcgt
     9541 ggtctgcccc tggcaccagt acaagtttca ccgttgcacg ggcgagggcg agccgggcta
     9601 cgaagcggat aaagttccag gctacggcgt caaggaagag aacggccggt tgttagtcac
     9661 cgcgaagccg accagcaagc gcgggcacct gccgcacgag ccgcatccgc tgacgcgctc
     9721 caccgatcgc gcacccggcg gtctgcgcgt cggtggcatc tccaccacgg caatggacgc
     9781 gaagaatccg cgtttctcga catcggaaca tctgctgaac gtggcgctcg agcacgcgag
     9841 cacggcgctc ggagcggaaa cgaagctgat ccggctcaac gacctgaatt ttcgcaattg
     9901 cgagggctac tactccaagg cggcgaaggc ctgcacgtgg ccgtgctcgg tcacctccat
     9961 ggacaagacc gaccagctcg accaggtgta cgaaatggcc gttcattggg ccgacatcct
    10021 gctgatcgcc acgcccattc gctggggcgc ggccagctcg ctgtattaca agatggtcga
    10081 gcgcatgaac tgcatccaga accagatcac cctcaacaac aaggtcctga tccgcaacaa
    10141 ggttgcagcg ttcatcatca ccggcggcca ggacggcatc caggccgtgg ccggacagat
    10201 gatggggttc ttcagcgaga tcggctatct acttccgcag tttccgttca ttgcgcattc
    10261 gctcggttgg gaattcgaag agatggagcg caacgtggac ttcgtgcagg ccagcgaagg
    10321 gctgcgtgcc ggcgctcgcg acctggtcgc tcgcgcgtcg gagatgtcga agtgtctgct
    10381 cgagcgggag atagctcgct ccaccatcga gaggggcggc cggaaggcgt ccgcttagcg
    10441 accgctcaat tcggcgtggt gaccgtgacc gcgttgaatt gccgcatctg gcgcagctgc
    10501 tggcgggtcg agcgcagcag ttcctcctgc atctcctcga agacacgaag ctgcgccggt
    10561 gtcagcactt cagctgcacg cgtgcgcatc cgctgactgt tttcccgcgc cgattccaac
    10621 tgcgcctccg gagactcgcc ggccatgatg atcgtgccgg tgccgctgcc aaagtgcatg
    10681 agtccgccgc cacgtttcga agcgtcgagt gaaatccggc tccgttcgtc cgcgaggacg
    10741 acgatgagcg cctccgcctg ctcttccggc aggaagtcgg catcagaaag ccgcgtgcgc
    10801 aactccgtca cggatgtgcg ttcgcccatg gtgctcctgt attgctcgaa tttctgccgg
    10861 ccctcggggc cgagcagatt tgcgagctcc cgttcgttcg caccggcgaa ctcacggccg
    10921 atacgctgaa agtcgcacga tgaatcgaca gagcaccgtg cgaatttctc ctgttggtcc
    10981 agctgctgtt gcgccagcaa ctcgatgagc cgttcggcct cctcgccgga catcttcagc
    11041 gtctgcgcga gacgcgggtt cgagttgcgc gccatcgtcc tgaattcctc gagcatttcc
    11101 gcgcgctgct ccggatcatc gagccgcttg attagctgct cctgatactt gcgctgctcg
    11161 gcctgaatca gtgcatatgt ggagccgggc accggctcgg tgctcgccat tgcgcctggt
    11221 cctgacaggg tggcgaaggt gatcgtggtg ggctcggacg tcggcgaagg tgcagatgtg
    11281 ggcgaggggg ctggttcgat ggccgccccg ggcgccgcta ccacggatga agtcgagtag
    11341 atgggtggct ctgcacccgc gttcgcgcgt tcgacttgca actcgcgcgc aaaatagacg
    11401 gtggacgcgg cgaatgccgc ggtggccaag gcgagcgcga tgacggctgt gcgcatgtgg
    11461 ctctatatat gccgagacac attgctcgtc tagctgatcc actctttgat gatgcgcggc
    11521 accacgcggg tagctggacc gggacggaaa acaaatccgc gcggcacttc gtccatctcc
    11581 agtgagttca gcaccttgtc cgcgtccgcg cgtgcatcat cgacgagcga agccgccgga
    11641 tacacgctga gcgaggtgcc aactaccagg atcttgtccg cggctgcgac atgctcgcgc
    11701 gcctcgtccc ggaattgggt catctccccg aaccagacga tgtcgggacg cagctgcgta
    11761 ccttcatcgc agagctggcc gatttcgatg ggcgctccgt cgatgcgata tcgccggcgt
    11821 gcggggcccg tgccacgggc aaatgccagt tcgccgtgta cgtggagtac gttcgtcgag
    11881 cccgcacgct catggagcgc gtcgacgttc tgcgtgacca caacgacgtc aaacttcgtt
    11941 tcgagcgccg cgatggccac gtgtgcggaa ttgggctgcg ctgcccaggc cttcgcacgc
    12001 cgctcgttgt agaactcgag cacgagcgcg gggttcctcc gccagccttc tggactagcg
    12061 agttcctgcc acgaatgttg cctccacagg cctgcggcat ctcgaaaggt aggcaggccg
    12121 ctctccgcgg aaaggccgga gccggagagg atgacgatcc tggggcgcat gctgggatag
    12181 cggcccatct acccgggcga cgaggacttt gcgaacttcg ccttgatagg gtcgaacagc
    12241 agcatgacga gtatggcccc atcgatcatg ctgaacagcg tttcgaccaa tgacatcagg
    12301 ggtgttgtga gcgagacgcc ctcccaggga ataaccacga ttcccgccac ggcaacgcca
    12361 atgaacgtcg cgcgcgccca acggcgaaac agcaggagtc caacacacga ggccaggtac
    12421 gccgcgagaa agcccagcac cagcgcgccg agcaggatct gcaggctcaa tgggccggaa
    12481 aagaggccac tgagaagcgg accagctccg tcgccatcca ggtattcatt caccgcatcc
    12541 ggaaaatccg gttcgaggaa actgatgccg atgctgagag cgaacagccc caacgagctc
    12601 gccaccaggc cgcgaaagac tttggtcatt tccatcgctc acgacccctt catggttttt
    12661 gctcggcggt ccgccggctc tccatgcggc gaaaatcgtg cagcaggtcg tcctgcagct
    12721 ggttgaacgt aatgagttgt tcaccggcca agacggtggc ggcgcgagca cgcgtgcgct
    12781 gcacgtacgc ctcggccgac gccattcggc cttcggctgg cagcgcgtcc gcgtagaaaa
    12841 gcgacatccg gtccacattg ctgaagccct ttatatgcgt gccttcgttg gcaaagtcgc
    12901 gcatcgcggc ttcgcgttcg gcggtcagcg cggccacgag agcgctcgac tgtgcgctcg
    12961 agagtggcgc cgtcgagggc agacgtgccg acaggccttt gacggacctg cgcgcgggtt
    13021 ccgtcatggc ccaattcttc atctcatcga ggccttcatc gccaagcaac tcctgaagag
    13081 cctgctttct cgaagcgagc aactcgctga gcgcgggagt gacgcaagcc ggatcgaggg
    13141 cgcaacgcga gatgaccgcg cgcttctcga gttcggtatc cgcgacttcc tcaatgagct
    13201 tctgccaggc gccgggggcg agattgcggt ccttgtcgaa tccctcgagc aacgctcgcg
    13261 tacgctcgat ctgctggtcg cgcagcctgg cgcgtcctcc cggagagtca tacttcccca
    13321 gaaaatccgt ggcctgcgct cgcatcgcgg atttctgcgc gtccgtcatg atccgcggtg
    13381 cttcgcgcga ttccagagcg ctcaccttgg tgacggcggc cttctgggcg ggctccgtga
    13441 ctgaagtggc tggcgcgatt ggcgctgctg gcgccgatgg cgtcgccacc accttcggcg
    13501 caggcgctcg gggcgctgat gcgatcgcca gctcgctcgc caggtgcgcc gagttctcgc
    13561 gttccatgac cagctgctgg cggaaataga cggcggcggc cgcgcagacg gccgtgctga
    13621 ccgcgagccc cgcgactagt ttgctcaaga ttcgactccc tccgcgcgcg ggcaagatac
    13681 cacgactatc tagaagccag gaaagccgga gcacccgccg ggaaggtgcc ggcgtgatgg
    13741 agcaggtcca ccacgccgga atttcagtct ctaaaagaag tgatgcctgt tggagccgtc
    13801 gtgatcaccg cgccgcatac agcagctctt gaaacctgcg cccggacagg caggggcaag
    13861 gatccttgcg gccgagcttc tcgatgagct cttttcgtcc gccaaccact cgattcccgc
    13921 gcttcacttt cgcttcggac gggaaaccgc gccttctctt actcgtgacc tcgaaagcac
    13981 tcggcttcga ggtgatttct catgcgtcgc atgggccacc tctttggcaa ccccgggatc
    14041 gggggcgcgg atggtaatga ggcgcgtccc cttttgccag caaatcgtga atgtccccat
    14101 ttacgacttt gcgcgctcgc cgaacgacag ggatgtctgc cgctgcgact gcgactgttt
    14161 ctgccccgtc atgcgctgca cgccgctgat gtcgcccgac gggaagtaaa tctcggcgag
    14221 tcgcgagtgc acgttggaaa tgtgcagctg gatgttgtcg atgagctcgt gaaggccggg
    14281 ctggtcgagc gtggccagcg gggcgcgctg gatgaacttg gcggcgcctt cgagaacctc
    14341 gagcacgctt tcgctgcgcg gcagcggtgt cagcgagctt tcgagctgca tgaggcagaa
    14401 ctggcaggag cgcgggaact ggtcgtcacg cagcaggaac tggagcacgt cggtgcgctt
    14461 cacgcgtgtg cgcatcttca ggcggtacat ctggtacccc gacagtgact tcagcacgct
    14521 catccactgc acggtctcga acggccgaag ctcgggagtt tccgccggga gtagttgggc
    14581 ggagcgcacg tcgacgatgc ggctggtcat gtcggctcgc tcgagattgc gccccagcgt
    14641 gctgaaggtg tgcgcgtcat tgcggttcat ggtgccgtcg agcatgccgg acaaggtctg
    14701 caaggtgacg actacgcgct tcaggaaatc gaagcgtgtg cgtttattga tgccagccga
    14761 gaggtccttg ttgaattcgg caaagaattc gttgagcagt tcccaggctt cggtaggcag
    14821 gatttcccgc agggtgcggg cgttctcgcg cgctgcggcc agcgaggagc agatggaacc
    14881 cggattctgc ttgtccgcaa tgagaaaatt gacgacgtct cgctcttccc ccttcgtttc
    14941 cctggcgtcg aactcggcgc gatttcccga gatgtccacc agcggcaacc agccgggcgc
    15001 gatgccaccg ggaaggtcca gcagcaggtt ggtgttcacg ccgatgatgc gcgcggtgtt
    15061 ttccgcgcgt tccagatagc gcgagagcca gtaaacgttt tcgacgactc tagctagcat
    15121 gggggttcac ggtgccgggt gaggaaagtg gggagtgcga acgggcgctt ccgctcgctg
    15181 gcgacctgca cgcactcccc actttcctca cccggcacct tccgtatcaa cgatccaggt
    15241 gtccttgctg ccgccaccct gcgaagagtt cactaccagc gaacccttgc gcagcgcgac
    15301 gcgcgtgagg ccaccggtgg tcaccgcggt ttgcgggccg gacaggatga acggccgcag
    15361 gtccacgtgg cgcggctcga gcctgttgtt caccagcgtg ggcacggtag acagggtcac
    15421 gatgggttgc gcgatgaagc tgcgcggatc cgccttgagg gagtcggcga acttggcgcg
    15481 ctcggctttg gtcgacattg gccccatgag catgccgtag ccgcccgagc cgtcgaccgg
    15541 cttcacgacg agctggtcga gattggcgag cacgtgatcc agctgcctgg cttcaccgca
    15601 gcggtacgaa ggcacgttgg gcaggatggc gtcctcgccc aggtagtact tgatcatgtc
    15661 gggcacgtac gtgtagacga gtttgtcgtc ggcgacgccc gcacccggtg cattggccag
    15721 tgcgactttg cccgcggccc aggcgcgcat caggcccggc accccaagtg aagaatcctt
    15781 gcggaacact tcctggtcga ggaagtcgtc gtcgatgcgg cggtagatga cgtcgacgcg
    15841 cgtccagccg cgtaccgtct gcatgtacac gcagtcgtcg ctatctaccg cgaggtcacg
    15901 gccttccacc agctcacagc ccatctgccg cgcgagatag gcgtgttcga agtacgccga
    15961 gttgtagatg ccaggcgtca gcaccacgac ctcgggccgg gtttccttgc gcggggaaag
    16021 ggcggtcagc atgtcgaaga gctgcgacgg gtagtcatcc acgggcagga tggcagcgct
    16081 ctcgaacagc tccgggaaca cgcgtttgac gacctgccgg ttctcgagca tgtaggaaac
    16141 gccagacgga acgcgcaggt tgtcttcgag cacgtaaacc gtgccgtcct tgtcgcggat
    16201 caggtccgag ccgcagatgt gcgcccaggt gccgagcggc ggcttgatgc cgcggacgtt
    16261 ggcgcggaag ttcttcgagg tggcaatgat cgaggacggc atcaggccgt ccttgatgaa
    16321 tttttgcccg tggtagacgt cgtcgatgaa cagattcagt gcggcagcgc gctggatgag
    16381 gcccgcttcg atcttttgcc attccttgcg ggagatggtg cgcgggatga tgtcgagtgg
    16441 ccaggcgcgg tcgatggagc cgtcctgctc gtggtagacc gtgaacgtga tgcccatggc
    16501 cttgatggcc agttcggccg cctgctgacg gaccgcgatc tcgtcgccgt ccatatccgt
    16561 caaataacgc atcagctgac gcccggctgc gcgtgcgccg ccgccggggc ccacgagctc
    16621 gtcgtggaat ccaggggagg ggtatgacgt ccagtcggtt ttcattgttg gtgtgcgtgc
    16681 tcgacccaaa aataccagag ccaaaagccc atgttcaaaa aagtgcgtgg gcttagtgtg
    16741 cgcgcatccc gatggtgccg cgggccgggt ttccggccat attggtgcgg catggctaac
    16801 aggcctcctg tgtagaatga ccgcgtgtcc atacatgtcg cactcaatca cgtaacgcac
    16861 tatcgctatg accggctcgt ggcgctcggg ccacaggtag tgcgcctccg gcccgcgcct
    16921 cactgccgca cgcgcatcat ttcctattcc ctgaaggtca cgccggccaa acacttcatc
    16981 aactggcagc aggatccgga ggctaactac ctggcgcgct acgtcttccc cgagaaggcg
    17041 aacgagctgc gcatcgaagt ggacctggtc gccgaaatgg ctgtgctcaa tcccttcgac
    17101 tttttcctcg agccttacgc ggaaaccttt cccttcgcct accagggcgc ggagaagcgc
    17161 gatctcgcgc catatctcgt gatgttccgc ggcgagccga agttcaaggc ctacctcggc
    17221 accatccccc gcgaccgcaa gcgcaccatc gatttcctgg tcgagctcaa cgcgaaactc
    17281 gcgaatgaca tcaagtacct catccgcatg gagcccggcg tgcagacgcc cgagctcacg
    17341 ctcacgaagt gttccggctc gtgccgggac tcggcgtggc tgctggtgca gctgctgcgg
    17401 cacatgggtc tcgcggcgcg attcgtctct ggctacctga tccagctcac ggccgacatg
    17461 aaatcgctcg atggcccgcc gggtccggag aaggacttca ccgacctgca tgcctggtgc
    17521 gaggtctatc tacccggcgc gggctggatc ggcctcgatc ccacctcggg cctgctggcg
    17581 ggcgagggac acattccgct ggcctgcaca cccgagcctg gcgctgccgc gccggtcacc
    17641 ggggcggtcg acaaggcaga agtcgaattc gaacatcaca tgaaggtgga gcgcatctgg
    17701 gaagcgccgc gcgtcaccaa gccgtacacc gaaacgcagt ggaaggaaat tgagtcactg
    17761 ggtcacgcca tcgacgcgca attgctcgcg ggcgacgtgc gcctcaccat gggtggcgag
    17821 cccacgttcg tctcggtcga tgatccggat ggcgcggaat ggaatacaac cgccctgggt
    17881 cccgacaaac ggcggctcgc agtcgaggtg tacaaccgcc tcaagcagaa gtacgcgccg
    17941 caaggcctgt cgcacttcgg ccagggcaag tggtatccgg gcgagccgct gccgcgatgg
    18001 tcgctcaact gtttctggcg caaggatggg cagcccatct ggcagaaccc cgagttgtac
    18061 gcggacgaga gcatcaagcg ggatatcgac gcgaccgtcg ctcagcgttt ccttgcaaca
    18121 gttgcgatgt cgctgggtgt ttcgcccgca ttcgtgtttg cagcttacga ggacacg
//
LOCUS       DASQJS010000284         8485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_173367, whole genome shotgun sequence.
ACCESSION   DASQJS010000284 DASQJS010000000
VERSION     DASQJS010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173367"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1421
                     /locus_tag="VEW08_11330"
     CDS             168..1421
                     /locus_tag="VEW08_11330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYJ41376.1"
                     /translation="MPAPEIARVSAEIDRIYRNDSRRIFATLIRLLGDFERAEEALHE
                     AFRAAMEKWPAEGLPQNPVSWLISAGRFKSIDKQRRDKRFDHLEDRPDVAEALADSAP
                     ALDEREELEDDRLRLVFTCCHPALSPDTQVALTLREVCGLTTEEIAAAYLTPAPTIAQ
                     RIVRAKAKIRDANIPYEVPGRDALPERLESVLRAVYLVFNEGYSASSGETATRADLSG
                     EAIRLGRLIVELVPEPEAQGLLALMLLHESRRETRATPDGELILLDEQDRARWNRALI
                     DEGAALVREALSTQRIGPYSVQAAIAAVHAEAPSAEETDWREIVGLYDLLLRITPSPI
                     VALNRSVAVAMRDGPEAGLVQLDQLLAEGALDGYQLAHAARADFCRRLGRYTEARDAY
                     RRAIKLTAQDPARRFLERRLAALPG"
     gene            1593..1958
                     /locus_tag="VEW08_11335"
     CDS             1593..1958
                     /locus_tag="VEW08_11335"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYJ41377.1"
                     /translation="MKYLCLVYLSPEEWNSAPDEACMACGDELRASGHFITGTPLHPV
                     HTATTVRVRNGQLAVTDGPFAETKEQLAGFYLIDARDLNEAIQIASRIPPAKYGSIEV
                     RPTRELQVSTQHAAAAAAP"
     gene            1982..2386
                     /locus_tag="VEW08_11340"
     CDS             1982..2386
                     /locus_tag="VEW08_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ41378.1"
                     /translation="MHSQIFVNLPVKDLKRSVLFFTGLGYTFNPTFTDENATCMIIGE
                     NLFVMLLVEKFFSTFTSKAITDTAKSTEVLNCVACSSREQVDSLVAKARAGGGKVPRE
                     PQDHGFMYSHGYEDLDGHTWELVHMTGEPPKQ"
     gene            2396..2821
                     /locus_tag="VEW08_11345"
     CDS             2396..2821
                     /locus_tag="VEW08_11345"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYJ41379.1"
                     /translation="MRYMIIVKASRDSEAGAKPTDALFARMAAYHEALGKAGILVDAS
                     GLQATSKGWRVEVHGEHRSIVDGPFAETKELIAGYTIIRVKSEQQARDWTARFPNPNL
                     DDGPWHIEVRRMFELEYFEPNPAVDVFRKLAGNGRPVGA"
     gene            2933..3820
                     /locus_tag="VEW08_11350"
     CDS             2933..3820
                     /locus_tag="VEW08_11350"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HYJ41380.1"
                     /translation="MNRPAAATVTTELDRVLHAAMADDSLELPPPPQVVSEVMRLTRG
                     EAAGADAPDASNTDLAQLIQRDVALAGQVMRVANSALYARRSPVVTLPQAIAWLGMRE
                     IRNIAFAVALKGQVFASAYFRTEMADLWRESVITALFAQEVARLKRRNVESAYLCGLL
                     HRAGMAVILHNVGAAVVRHRLTPGTEQVLQLAARHEARVGTHLSIAWSLPPAVAAAIA
                     HWSNPPAAELSRTEVMEVALARQISDELRAPGVGGELPDSMLGPVSKWMDELSIYPDE
                     LFTIMAQRAAIYATAESYT"
     gene            3817..5247
                     /gene="sthA"
                     /locus_tag="VEW08_11355"
     CDS             3817..5247
                     /gene="sthA"
                     /locus_tag="VEW08_11355"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HYJ41381.1"
                     /translation="MSLPSCAAEIFDVVVIGSGPAGQKAAIQAAKAGRRVAVIERDRK
                     VGGSCVHTGTIPSKSLREHALRQRVRHVDLMGEPIQSLLDGVGATIAAHDTYMAAQLE
                     RNHVRLLRGRANFTAPNELAIRRIDGSNAVVRAGIIIIATGSRPRTPSHLAVDHEHVL
                     DSDSILSLAYLPRSLLVLGGGVIGSEYAATFAALGCKVTQADQYERPLGFLDPELTSF
                     YVDELRRNGGEYIPNSEAVATTWDGVSQVRTDFKDGRTLFADKVLVALGRVANVDGLT
                     LDAAGIALTSRGHIQVNDELKTTATGVYAAGDVIGPPALASVSMEQGRRAACHALGLL
                     VPSDPVSRLPSGVYTIPEIATVGLDEHEARRHFGGAIVGRARFEEIARGQINGCQRGI
                     LKLVADPGGRRVVGVQIAGDSATELVHIGQLGLAAELDVDAYVDNVFNFPTMAEAYRI
                     AAFDIVKQRGRATGNENVADVLAAVL"
     gene            5339..5512
                     /locus_tag="VEW08_11360"
     CDS             5339..5512
                     /locus_tag="VEW08_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41382.1"
                     /translation="MSFALYMFGLAVLLGGIAWGLITAGLAVTYVVIACLIIGGIGIM
                     MAVSRTRAKDPPV"
     gene            complement(5509..5928)
                     /locus_tag="VEW08_11365"
     CDS             complement(5509..5928)
                     /locus_tag="VEW08_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYJ41383.1"
                     /translation="METLMKVDADLVKRMRETKSWSQEHLATVAGVSHRTVQRVESDG
                     TASLETRMALASALGVSAADLGPKPGRAPLPLGTRIGFACGVAGAVAGMAFGWMGAVA
                     SGAAGQDAGAAYGTMAALTGITCAVIGVVANRLWRLG"
     gene            complement(6009..6401)
                     /locus_tag="VEW08_11370"
     CDS             complement(6009..6401)
                     /locus_tag="VEW08_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41384.1"
                     /translation="MNKTKSISLLLAIGAVLEIPVGLGLLIAPSPLATLLLGAPLTGA
                     GLVVARLAGGGLLALGIACWFARSTPTAPASLGVAGALLIYNIVACVTLALALPPDLP
                     GYALTLGVTVLHGLVAAGLLAALGARLS"
     gene            complement(6511..6837)
                     /locus_tag="VEW08_11375"
     CDS             complement(6511..6837)
                     /locus_tag="VEW08_11375"
                     /inference="COORDINATES: protein motif:HMM:NF025161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HYJ41385.1"
                     /translation="MAFTGIQSPSDISAALSASVERGGLVLDEKDLTPDFFDLKTGFA
                     GQVLQKFVSYRTKLAVIVRDAQVYGGRFGELMHEHRIHPTVRFFSAEQAARQWLSQMP
                     SGKRSS"
     gene            complement(6848..7327)
                     /locus_tag="VEW08_11380"
     CDS             complement(6848..7327)
                     /locus_tag="VEW08_11380"
                     /inference="COORDINATES: protein motif:HMM:NF018748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYJ41386.1"
                     /translation="MNAPGQGRPPFPPFTSETAAQKARAAEDAWNSRDPERVSLAYTE
                     DSAWRNRAEFFSGRPAIVEFLKRKWARELDYRLIKEVWAFEGARIAVRFAYEWHDDSG
                     HWFRSYGNENWEFDELGLMRRRHASINDLVISEADRQFRWSLGRRPDDHPRLSALGL"
     gene            complement(7327..>8485)
                     /locus_tag="VEW08_11385"
     CDS             complement(7327..>8485)
                     /locus_tag="VEW08_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ41387.1"
                     /translation="QEYKITGLNDDSDVTGFDDTFWLTKQWRVDRDMAAYGEVTFNAT
                     DRLAFTGGARYFRTENSLEGFFGFGATNPYGSSTGEGSCFDTGQMFETVPCTNLDRTV
                     KQNDTTFRFNTTFHATDDVMFYATWSEGFRPGGVNRRGTFPPYKADFLTNYELGWKTT
                     WADNRLRFNGAIFKGDWDDFQFSFLGENGLTNISNAGNAQLEGIEVDVQWAATDAFML
                     YGGLAVQKAELGEDFCKTLDANGDPLNEADCIANEPADFAPEGTSLPTTPEFKASLTG
                     RYEFQMNGLDAHFQASVVHNGEAATALLPAENDVLGDSEAYTLVDFSFGFEREKWSAE
                     LFIDNVFDERVALYRYSECDVTICSNPFEEGIVYGVVTRPRMVGFKFSQRF"
BASE COUNT         1528 a         2853 c         2642 g         1462 t
ORIGIN      
        1 cccctcgtgt tcgtcttcag tctggccctg ctctcgtacc tgatcgccct gctctgcgga
       61 gaactggcgc tggtcggcga cggagtgagc gacgcggccc ggcgcgcgcg ctgaagtccg
      121 gccccttttg ctaggaaatg gggtctgacc ccatttatcc tgccggcatg cccgcccccg
      181 aaattgctcg cgtcagcgcc gagatcgaca ggatttaccg caacgattcg cgccgcatct
      241 tcgcgacgct gatccgcctg ctcggcgact tcgagcgcgc ggaagaagcg ctgcatgaag
      301 cctttcgcgc cgccatggag aagtggccgg ccgagggact gccgcagaat cccgtttcct
      361 ggctgatctc cgcaggccgc ttcaaatcca tcgacaagca gcggcgcgac aagcgcttcg
      421 atcatctcga ggatcggccc gacgtcgccg aagcgctcgc cgactccgca cccgcactcg
      481 acgaacggga agaactggaa gacgaccgcc tgcgcctggt cttcacgtgc tgtcatccgg
      541 cgctctcgcc cgacacgcaa gtggcgctca cgctgcgcga ggtctgcggc ctgactaccg
      601 aagagatcgc cgccgcgtat ctcacccccg caccgacgat cgcgcagcgc atcgtgcgcg
      661 ccaaggccaa gatccgcgat gccaacatcc cgtacgaagt ccccggtcgc gatgccttac
      721 ccgagcgtct cgaaagcgtg ttgcgcgcgg tctacctggt gttcaacgaa ggttattcgg
      781 cgagcagcgg cgagacggcc acgcgcgcgg acctgtccgg cgaggcgatc cgcctcggcc
      841 ggctcatcgt cgagctcgtg cccgaacccg aagcacaggg gttgttagcc ctgatgctgc
      901 tgcatgagtc acgacgcgag actcgcgcga cgccggacgg cgagctgatc ctgctcgatg
      961 agcaggaccg cgcgcggtgg aaccgtgcgc tcatcgacga aggcgcagca ctggtgcgtg
     1021 aggcgctgtc cacgcaacgc atcgggccgt attcggtgca ggcggccatc gccgcggtcc
     1081 acgccgaggc gccttccgca gaagaaacgg actggcgcga gatcgtcggg ctctacgacc
     1141 tgctgttgcg gatcacgcca tcgcccatcg tcgcgctcaa tcgcagtgtg gcggtcgcga
     1201 tgcgcgacgg gcccgaggcc ggcctcgtgc aactcgacca gttgctggct gaaggcgcgc
     1261 tcgatggcta ccagctggca cacgcagcgc gcgccgactt ttgccgccgc ctggggcgct
     1321 ataccgaggc gcgcgacgcc taccggcgag ccatcaagct caccgcccag gacccggcac
     1381 gtcgcttcct ggagaggcgg cttgcggccc tgcccggctg acgagccact gggtgccggg
     1441 tggggattcc ggggagcggc ttgcggccct gcccggctga cgagccactc cccggaatcc
     1501 ccacccggca cccacccaag ggagtttttt cgcagaccgt tgtcgaaagc ccattcgcca
     1561 gttcgaccaa ctctcaaccg aaggagattc gcgtgaagta cctctgcctg gtttacctct
     1621 cgcccgaaga gtggaattcc gcccccgatg aagcctgcat ggcctgcggc gacgagttgc
     1681 gcgccagcgg gcatttcatc accggtacac cgctgcatcc cgtgcacacc gccaccaccg
     1741 tgcgcgtgcg taatggccag ctggcggtga ccgacgggcc gttcgccgaa accaaggagc
     1801 agctcgcggg cttctatctc atcgacgcgc gtgatctcaa cgaggccatc cagatcgctt
     1861 cgagaattcc gccggcgaaa tacggaagca tcgaagtgcg ccccacgcgc gagctgcagg
     1921 tgagcactca acacgccgcc gcggccgccg cgccctgacc tgccaaccaa ggaacgacat
     1981 catgcattcg caaattttcg tcaatctccc ggtcaaggac ctgaagcgct ccgtactctt
     2041 ctttaccggg ctcggctaca cgttcaaccc cacgttcacc gacgagaacg cgacctgcat
     2101 gatcatcggc gagaacctgt tcgtgatgct gctggtcgag aagttcttca gcaccttcac
     2161 cagcaaggcg atcaccgaca cggcgaagtc cactgaagtg ctcaactgcg tcgcctgcag
     2221 cagccgcgag caggtcgatt cgctcgtcgc caaggcacgc gccggcggcg ggaaggtacc
     2281 gcgcgagccg caggatcacg gattcatgta ctcccatggt tatgaggacc tcgacggcca
     2341 cacctgggaa ctcgtgcaca tgaccggcga gccgccgaag cagtaggagc cgcacatgcg
     2401 ctacatgatc atcgtcaaag cttcgcgcga ctccgaagcc ggcgcgaaac cgacggacgc
     2461 gctgttcgcg cgtatggcgg cttatcacga ggcgctcggc aaggccggca tcctggtcga
     2521 tgcctcgggg ttgcaggcga cctcgaaagg ctggcgcgtc gaagtgcacg gcgagcaccg
     2581 aagcatcgtt gacggcccct tcgccgagac caaggagctc atcgcgggct acaccatcat
     2641 ccgcgtgaag agcgagcaac aggctcgcga ctggaccgca agattcccta acccaaacct
     2701 cgatgacggc ccctggcaca tcgaggtgcg ccgcatgttc gagctcgagt atttcgaacc
     2761 gaacccggcg gtcgacgtgt tccgcaagct ggccggtaac ggcagacccg tcggcgcgta
     2821 accccttgat tttgtgacgt gagttgaggt cgcggcggct cgttgccgca gcgagtcaag
     2881 tgactgaatt cccggccgtt aacgggacgg gtcactccac caacgacacg ccatgaacag
     2941 accagccgct gcgaccgtta ccactgaact cgaccgtgtt ctgcatgcgg ccatggcgga
     3001 cgattccctg gagctcccgc caccgcctca ggtcgtgtcc gaagtcatgc gtctcacgcg
     3061 cggcgaagcc gccggcgccg acgcgccgga cgcgagcaac accgacctcg cgcagttgat
     3121 ccagcgcgac gtcgcgctcg ccggacaggt catgcgcgtg gcgaactccg cgttgtatgc
     3181 gcggcgctca cccgtggtca cgctgccgca ggccatcgcg tggctcggta tgcgcgagat
     3241 tcgcaacatc gcatttgccg tggcgctcaa gggccaggtg ttcgcctcgg cctactttcg
     3301 caccgagatg gcggacctgt ggcgcgagtc ggtcatcacc gcgctgtttg cgcaggaagt
     3361 cgcacggctc aagcgccgca atgtcgagtc ggcctatctc tgtggattac tgcatcgcgc
     3421 cggcatggcg gtgatcctcc acaatgtcgg cgccgccgtc gtcaggcatc ggctcacgcc
     3481 gggtaccgaa caggtactgc agctcgcggc gcgccacgag gcccgcgtgg gcactcatct
     3541 cagcatcgcg tggtcactgc ctccggcggt ggccgccgcg atcgcgcact ggagcaatcc
     3601 gccggcggcg gagttgtcac gcaccgaagt catggaagtg gccctggcgc ggcagatttc
     3661 cgacgagctg cgcgcgcccg gcgtcggcgg cgagcttccg gactccatgt taggcccggt
     3721 ttcgaagtgg atggacgaac tgtccatcta ccccgacgag ctgttcacga tcatggcgca
     3781 gcgcgccgcc atctacgcca ccgcggaaag ttacacatga gcctgccatc ctgcgccgcc
     3841 gagatcttcg acgtcgtagt gatcggctcc ggaccggcag gccagaaggc cgcgatccag
     3901 gcagcgaagg ccggccggcg cgtcgccgtc atcgagcgcg accggaaggt cggcggctcg
     3961 tgcgtgcaca ccggcaccat cccgagcaag tccttgcgcg aacacgcgct gcgccagcgc
     4021 gtgcggcacg tggacctcat gggcgagccg atccagtcgc tgctcgatgg cgtgggcgca
     4081 accatcgctg cgcacgacac gtacatggcg gcgcagctcg agcgcaatca cgtccgtctg
     4141 ctgcgcggcc gcgcgaactt cacggcgccg aatgagctgg ccattcgccg catcgacggt
     4201 tctaacgcgg tggtgcgcgc cggcatcatc atcattgcca ccggctcgcg gcctcggaca
     4261 ccatcgcacc ttgccgtcga tcacgaacac gtgctcgaca gcgattcgat tctctcgctg
     4321 gcctacctgc cgcgcagcct gctcgtgctg ggtggcggcg tgatcggcag tgaatacgct
     4381 gccacgttcg ctgccctggg ctgcaaggtg acccaggccg accagtacga gcggccgctg
     4441 ggttttctcg atcccgagct gacctcgttc tacgtcgacg agctgcgacg caatggcggc
     4501 gaatacattc caaactccga ggccgtggcc acgacatggg acggcgtctc gcaggtacgc
     4561 accgacttca aggacggccg caccctgttc gccgacaagg tgctggtggc tctcggccgc
     4621 gttgccaacg tcgacggcct cacgctggac gctgccggca ttgcgctcac cagccgcggt
     4681 cacatccagg tgaacgacga gctcaagacc accgcgaccg gggtctacgc cgcgggcgac
     4741 gtcatcggcc cgcccgcgct cgcatccgtc tcgatggaac agggacgccg tgccgcgtgt
     4801 catgcgctcg ggttgttagt gccctcggat ccggtatcgc gcctgccctc gggcgtctac
     4861 accatccccg agatcgccac cgtcgggctc gacgagcacg aggcgcgccg gcactttggt
     4921 ggcgccatcg tcggacgagc gcggttcgaa gagatcgcgc gcggccagat caacggttgt
     4981 cagcgtggca ttctgaagct ggtggcggat cccggtggac gacgcgttgt cggcgtgcag
     5041 atcgccggcg acagcgccac ggagctcgtg cacatcgggc agcttggtct cgccgcggag
     5101 ctcgacgtgg acgcgtacgt cgacaacgtc ttcaacttcc cgacgatggc ggaggcgtat
     5161 cgcattgccg cgttcgatat cgtgaagcag cgcggtcggg ccacgggaaa tgaaaatgtc
     5221 gcggatgtgt tagccgcggt cttgtgaagc gggtaggacc ggacctacgc caaccctggc
     5281 cttcgctcga tgaggcaccg cgaatgccct gcgtatactt tccgcagagg tgataccaat
     5341 gtcattcgcg ctgtacatgt tcgggctggc ggtgcttctg ggcggcatcg cctggggtct
     5401 catcaccgca ggactcgcag tgacctatgt cgtgatcgca tgcctgatca ttggcggcat
     5461 cggcatcatg atggcggtct cgcgtactcg cgccaaggat ccgccggtct aacccagtcg
     5521 ccagaggcgg ttggcgacca cgccaatcac ggcgcaagtg attcccgtga gcgcggccat
     5581 cgtcccgtac gccgccccgg catcctgacc tgccgctcca ctcgcaacgg cgcccatcca
     5641 gccgaacgcc atgccggcca ccgcgccggc cactccacaa gcaaagccaa tcctggtgcc
     5701 cagcggcagc ggcgctcgac ccggctttgg gccaaggtcc gcggccgaca caccgagcgc
     5761 ggaagccaaa gccatgcgcg tttccaacga agctgttcca tcggactcga cgcgctgaac
     5821 cgtgcgatgg ctgacgccag ccacggtcgc caggtgctcc tgcgaccagg atttcgtttc
     5881 tcgcatgcgc tttacaagat ccgcatcgac tttcatgagt gtttccatgg ttgtgctcct
     5941 cgcccgttaa aggaacggga agcgtcgcgt ccagctagct agtacgccac gacaagaggc
     6001 cgccagtttt acgacaagcg tgcgccaagg gcagccagca aaccggcagc gaccagtccg
     6061 tgcaatacgg tcacacccag cgtcagtgca taaccaggca gatcaggcgg cagcgccaga
     6121 gccagcgtga cgcaagcgac gatgttgtag atcagcagcg cgcccgcaac cccgagactc
     6181 gccggagcgg tgggtgtcga acgggcgaac cagcaggcga tgcccagtgc caggaggcct
     6241 ccgcccgcaa gtcgggcgac gaccaggccc gcaccagtca acggtgcgcc gaggagcaga
     6301 gtcgccagcg gcgacggggc aatcagaagt cccaggccaa ccggaatttc cagtactgcg
     6361 ccaatcgcga ggaggagtga aatgcttttg gttttattca tggcagttgc aaccatggat
     6421 gttgacgtcg aaatgaccgt cgatcgcgag cttgatcagg tctccacgaa tcgtcttcat
     6481 gggtatcaag ccgttcagcc ccgcctcagg ctagctggag cgctttcccg acggcatctg
     6541 cgacagccat tggcgcgcgg cctgctcggc actgaagaaa cgcacggtcg ggtgaatccg
     6601 atgctcgtgc atgagctcgc cgaagcgccc accgtatacc tgcgcatcgc gcacgatgac
     6661 ggcgagtttt gtccggtagc tcacgaactt ctgcagcacc tgcccggcga acccggtctt
     6721 gaggtcgaag aagtcggggg taaggtcctt ctcgtcgagc accagcccgc ctcgctcgac
     6781 actcgcggaa agggcagcgg aaatatcgct gggggactgg atgccggtga atgccatggc
     6841 agggagtcta caggcccagc gcgctcaacc ttgggtgatc gtcaggtcta cgtcccaaac
     6901 tccatcgaaa ttggcggtcc gcctcggaaa tcaccaggtc gttgatgctg gcgtgccgcc
     6961 ggcgcatcaa tccgagctcg tcgaactccc aattctcatt cccgtaagag cggaaccagt
     7021 ggccggagtc gtcgtgccac tcgtaggcaa accgcaccgc aatacgcgcg ccttcgaagg
     7081 cccaaacctc cttgatgagc cggtaatcga gctcccgcgc ccatttgcgc ttcaggaatt
     7141 cgacaatggc gggtcggccg gagaagaact ccgcccgatt gcgccaggcg ctgtcctcgg
     7201 tatacgccag cgacacgcgc tccggatcgc gcgaattcca cgcgtcttcg gccgccctcg
     7261 ccttctgcgc ggcggtttcg gacgtgaacg gcggaaacgg cgggcgtccc tgcccgggcg
     7321 cgttcatcag aatctctggc tgaacttgaa gccgaccatc cgcgggcgtg tcaccacacc
     7381 gtagacgatg ccttcctcaa acggattgga gcagatcgtg acgtcgcatt ccgaatagcg
     7441 atacagagcc acgcgctcat cgaacacgtt gtctatgaac agctccgccg accacttctc
     7501 ccgctcgaag ccgaacgaga aatcgaccag cgtataggcc tccgaatcgc cgagcacgtc
     7561 gttctccgcc ggcagcagcg ccgtggcggc ttctccgttg tgcacgacgg aggcctggaa
     7621 gtgcgcatcg agcccgttca tctggaactc gtagcggccc gtgaggctgg ccttgaattc
     7681 cggtgtcgtc ggcaggctcg tgccttccgg cgcgaaatcc gcgggctcgt tggcaataca
     7741 gtccgcctcg ttcagcggat cgccattggc gtccagcgtc ttgcagaagt cttcgcccag
     7801 ttcggccttc tgcaccgcca ggccgccata cagcatgaac gcgtcggtgg ctgcccactg
     7861 cacgtccact tcaatgcctt cgagctgcgc attgccggca ttggatatgt ttgtcaggcc
     7921 attttcgccg aggaacgaga actggaagtc gtcccagtcg cccttgaaga tggcgccgtt
     7981 gaagcgcagg cgattgtccg cccacgtggt cttccagccc aactcgtagt tggtgagaaa
     8041 gtccgccttg tagggcggga acgtgccgcg gcggttgacg ccgccgggac ggaagccttc
     8101 cgaccaggtg gcgtagaaca tcacgtcgtc ggtggcatgg aacgtggtgt tgaagcggaa
     8161 cgtggtgtcg ttctgcttca cggtgcgatc caggttggta cacggtaccg tctcgaacat
     8221 ctggccggtg tcgaagcagg atccttcacc ggtgctcgat ccatacggat tggtcgcgcc
     8281 aaacccgaag aatccctcca gcgaattctc cgtgcggaaa taacgcgcgc ctccggtgaa
     8341 cgccagacgg tcggtggcgt tgaacgtaac ctcgccgtac gccgccatgt cgcggtccac
     8401 gcgccactgc ttcgtcagcc agaacgtatc gtcgaagccg gtgacgtcac tgtcgtcgtt
     8461 gagacccgtg atcttgtatt cctgc
//
LOCUS       DASQJS010000285         9432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2056469, whole genome shotgun sequence.
ACCESSION   DASQJS010000285 DASQJS010000000
VERSION     DASQJS010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2056469"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VEW08_11390"
     CDS             <1..368
                     /locus_tag="VEW08_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41388.1"
                     /translation="TMSRVYDRLESATLLLARSGAIKDRLSAAWRQCLSNIEPEDVPR
                     ELRLHYLELASAMQRERPLRGEDAVRATIRKMSNEEAEGHAAFIVRMFCRMTRQQELE
                     LPLPSQASAAVVQLFAAEG"
     gene            complement(377..802)
                     /locus_tag="VEW08_11395"
     CDS             complement(377..802)
                     /locus_tag="VEW08_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41389.1"
                     /translation="MTHAWEITDDYLRIFEYLESSRNFFYVNLSAPDKAPKGGGKEAD
                     REELRRQMAQAECVIALPGLYRQQSELMLFELTFAKASDKPVILMRPFGANAVLPKEI
                     TSLSDQIVDWDGRALVDAVKAQARHEESNRWDTIEFKLD"
     gene            929..1693
                     /locus_tag="VEW08_11400"
     CDS             929..1693
                     /locus_tag="VEW08_11400"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYJ41390.1"
                     /translation="MRLLVYNIRYATGIGPAFHLPFPGAGYLRSSRKVLDGITSFVKA
                     QDPDIVGLIEVDIGSVRSGMVNQAEHIADALGHYTTYECKYGKSSLNNFMPIVRKQGN
                     AFLAAPRVEGERFHYFDTGIKRLIIELELDDVCIFLVHLALKYRQRQYQLRYLHDLII
                     KSHKPVIVTGDFNTFWGTHEIYLFMRAAGLRSANEKNVPSFPARIPRIELDFVLVSKE
                     IEITHFEVPDVRWSDHRPVLCDFNVRGAVASQPAVA"
     gene            1714..3759
                     /locus_tag="VEW08_11405"
     CDS             1714..3759
                     /locus_tag="VEW08_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HYJ41391.1"
                     /translation="MTTSATGAWALERDAEGIAWLTFDKPGSSTNVLSRETLVELDSH
                     LESFNGSPPRGVVIRSAKKSGFIAGADVREFTQLTSAEQAFELVRAAQRILDHLESLP
                     CPTVAIIHGFALGGGLELALACRYRVGIRSDHFSIGLPEVMLGIHPGFGGTVRAVRRA
                     GVRTAMEMMLTGKTLRADRARNAGFLDRLVYPADAEAAARELINRQPAQRRPRLLDRM
                     LSWPLVRGLVKKQLTAQVRAKARPEHYPAPYAIIDLWSTHGARGAAAYEAEARSIAQL
                     MLSDTSRNLVRVFLLQDRLKNLGGKSKVPLARVHVVGAGVMGGDIAAWCAQRGLNVTL
                     QDRELKFIEPALTRAREGFAKRLKDKDKAAVLMTRMTADVAGDGVPGADVVIEAIFEN
                     AEAKHELFGRLEPRMKTSAILASNTSSIMLEQLDDRLPDPARLVGLHFFNPVAQMPLV
                     EIVRGEHSSEESVQKAIAFARKIDKLPLPCKSAPGFLVNRVLVPYLYEAMFALQDGIP
                     IEVIDEAALRYGMPMGPVELADVVGLDVCKHVGDIVSKALGKQQPDTARIDALIAAKK
                     LGRKSGEGFYVWRDGKAVKSKGPHAEPPADLEDRLVLALANECAAVLREGIVADADLI
                     DAGVIFGSGFAPFRGGPLTYARKRGVEVVVARLVELTKTYGSRFTPDAGWTSLSTVP"
     gene            3790..4866
                     /locus_tag="VEW08_11410"
     CDS             3790..4866
                     /locus_tag="VEW08_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYJ41392.1"
                     /translation="MSDAREVSVELLRTLTPLEGMKRENLHALAKKVTVKQMNAGRLL
                     FKEGDADKRTIWIIKGLVEISEQGRTTAMIEGGSADARAPLNQSVPRKVTARAVEDVD
                     YLAIDADLLDVMITWDQTGNYEVGELQAQFGGGNEDSDWMTTLLQTKAFHRIPPANLQ
                     AIFMRMQRTPYRAGEVVIKQGDEGDYFYAIVQGRCLVTRESPLNQKGIKLAELGVGET
                     FGEEALIAEAKRNATVSMLTEGVLMRLNKQDFRDLMHEPLLRWVDEAQALKIIQNRGQ
                     WLDVRLPSEYQNLAIEGSINIPLYFVRLKLNALDRKTPYVVVCDTGRRSSAAAFILSE
                     RGFDSYVLKGGITTSNLPMRRPGG"
     gene            complement(4826..5605)
                     /locus_tag="VEW08_11415"
     CDS             complement(4826..5605)
                     /locus_tag="VEW08_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41393.1"
                     /translation="MRSVMCLMLLTTFPALAAAASPCVPAPRAPGTLIYAPHAAFEAY
                     VVEVHEPQDSRSAFGVLHARRALVEVVRSFHGPYSAGQQVETLTIETMRGCAVSVEEG
                     GHVMVVSESGGPFEIVESLPKAQVVPKGPFAALAYAHGEPRRSLRPSLKNASLHGEID
                     TGLAVELRARAHPGPREHCEIVSDGNYAQVSWGKAFGGNDARFKVIFERASGGWIEIL
                     RYQAPAPTPARNRNKRTRQRALWAEVSGVSRPGVASANSTS"
     gene            complement(5823..6188)
                     /locus_tag="VEW08_11420"
     CDS             complement(5823..6188)
                     /locus_tag="VEW08_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003179413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HYJ41394.1"
                     /translation="MTERTGRTGANKPGLLTLTIKDKSALYLAYMPFVKNGGLFIPTN
                     SNYRLGDEVFMLLNLMGEDEKLPVAGRVIWLTPKGAQGKRTAGIGVQFSDQDRGQTQK
                     KIETYLAGALAGDKATHTM"
     gene            complement(6185..7162)
                     /locus_tag="VEW08_11425"
     CDS             complement(6185..7162)
                     /locus_tag="VEW08_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41395.1"
                     /translation="MPGTAARRGLVSTLPLPSWFATPFGEVNAAIAADRLAHGLLIHE
                     DPGAGGMQLAHWIAQRVNCRESSRAPCGECQECRWIAADQHPDVTRLSPEEDSQYIKI
                     EAVRELTSDLTLTAHGRGYKVAIFSPADALHPNAAQSLLKTLEEPAKRTLLLLVTSQP
                     SRLLPTVRSRCSRLRLAGPTREVAANYLEAARGAGPWAEALAATGAGPFTLLDADPAA
                     LAQLRTDTISTLQDIGSGNLQPPAVAERWAKGELSIRLSCLESWVTDRILESAAIRDV
                     THLSGAALAPKICRLFEFSDAIRDMRKLAHTSINKTMAVEALLWRWARP"
     gene            complement(7129..7776)
                     /gene="tmk"
                     /locus_tag="VEW08_11430"
     CDS             complement(7129..7776)
                     /gene="tmk"
                     /locus_tag="VEW08_11430"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HYJ41396.1"
                     /translation="MMPAKFITLEGIEGAGKTTVADRLTQSLRARGITIHATREPGGT
                     KLAERVRALVLERGEEHISATAETLLMFGARQVHVDNLIRPALTRGEWVLCDRFTDAT
                     HAYQGGGRGVDRKLIDQIAHAVHGDLNPDCTILLDVPVRVGLERMQARRGAVDRFEIE
                     SSQFFDRVRNRYLELARAFPARFRVIDATAKLDQVCAAAIAALDECLALPPAAVS"
     gene            complement(7773..8798)
                     /gene="mltG"
                     /locus_tag="VEW08_11435"
     CDS             complement(7773..8798)
                     /gene="mltG"
                     /locus_tag="VEW08_11435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HYJ41397.1"
                     /translation="MRKLLLILLGLGVIAGAAATIYWQRANAVMDAPGPHQQPVALLV
                     KPGATVRAALAELETRGALADRRAVELVMRVRGWPQIKTGHYEIPALASPREILRQLS
                     EGRVVLETLTVIEGWTFADMRRAVEAHAQIKVTMRGRQIAEIMSAIGHAGEHPEGRFF
                     PDTYRFAGGTTDRDLYALAYRKMAETLEAAWSARAAALPLANAYEALTLASIVEKETG
                     LASERPRIAGVFTTRLRRNMRLQTDPTVIYGLGPSYDGNIRERDLRTDTPYNTYTRGG
                     LPPTPIALPSREAIEAVTRPLETGDIFFVATGIGDGSHEFSATLEEHNAAVARYLARL
                     RGASVPR"
     gene            complement(8791..>9432)
                     /locus_tag="VEW08_11440"
     CDS             complement(8791..>9432)
                     /locus_tag="VEW08_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HYJ41398.1"
                     /translation="SLAMLRAEIDAAVAVAPPLAVLKIIVTRGSAQRRGYAPQGDEIP
                     RRLVSLWRAPPRDAALVDGVTLCVASIRLGENPALAGIKHLNRLENVLAAAESASRGV
                     FDSLLLDASGNLVSGAMSNVFIVRDGCVTTPLIQSCGVAGVMRGIVLRECAALGLAAA
                     EERLTLDDLFSAAGVFITNARIGVVPVRRVGEHLYDMNNLAKRLAAHIEPLDA"
BASE COUNT         1712 a         3015 c         2978 g         1727 t
ORIGIN      
        1 acaccatgtc gcgtgtctac gatcggctcg aatcggccac tctgttactg gcgcgttccg
       61 gcgccatcaa ggatcgactc agcgctgcct ggcgccagtg cctctccaat atcgagcccg
      121 aagatgtccc tcgcgagctg cgcctgcatt atctggaact ggcttcggcg atgcagcgcg
      181 aacgtccgct gcgcggtgag gatgccgttc gggccaccat ccgcaagatg tccaacgagg
      241 aagccgaggg ccacgcggcg ttcatcgtgc gcatgttctg ccgcatgaca cgccagcagg
      301 agttggagct gccgctcccc tcgcaggcca gcgccgcggt ggttcagctg ttcgcggccg
      361 agggctgact ctctagctag tcgagcttga actcgatggt gtcccagcgg ttcgactctt
      421 cgtggcgcgc ctgggccttg acggcatcca cgagcgcccg gccgtcccaa tcgacgatct
      481 gatccgacag cgacgtgatt tccttcggca gcacggcatt cgcgccgaaa ggccgcatga
      541 gaattacggg tttgtctgag gctttcgcga aggtcagctc gaaaagcatg agttcgctct
      601 gctgccggta cagccccggc aacgcgatta cgcactccgc ttgcgccatc tggcgccgca
      661 attcttcgcg atcagcttcc ttgccgccgc ccttgggcgc cttgtccggc gccgacaggt
      721 tcacgtagaa gaagttccgg gagctctcga gatactcgaa tatccgcaga tagtcgtccg
      781 tgatctccca tgcgtgagtc acgaacactc gaattggatt gttttgtgac atgtcgaccg
      841 atccaaagcc ctgccttgag tgtagtttag tggcttgcgt ttggcgccct ctccactttt
      901 gtgcgccgca acgttagcat cactccgctt gcgcctgctc gtctacaaca ttcgctacgc
      961 cacgggcatt ggaccagctt tccatttgcc gttcccggga gccggctatc tacgttcgag
     1021 ccggaaggta ctcgacggca ttaccagttt cgtcaaggcc caggacccgg acatcgtcgg
     1081 gctcatcgaa gtcgacatcg gttcggtgcg cagcgggatg gtgaaccagg ctgagcacat
     1141 cgccgacgcc ctcgggcact acaccacgta cgagtgcaag tacggcaaga gctcgctcaa
     1201 caatttcatg cccatcgtgc gcaagcaagg caacgcgttc ctcgcggcgc cacgcgtcga
     1261 gggcgaacgc tttcactact tcgataccgg catcaagcgg ctcatcatcg agctcgaact
     1321 ggacgatgtc tgcattttcc tcgtgcacct ggcgctcaag tatcgccagc ggcagtacca
     1381 gcttcggtac ctgcatgacc tcatcatcaa gtcacacaag ccggtgatcg tcacgggtga
     1441 cttcaacacc ttctggggta cgcacgaaat ctacctgttc atgcgcgccg ccggactgcg
     1501 cagcgccaac gaaaaaaacg taccttcgtt tcccgcgcgc attccgcgca tcgagctcga
     1561 cttcgtgctg gtgagcaagg aaatagaaat cacgcacttc gaagtcccgg acgtgcgctg
     1621 gtcggaccat cgcccggtgc tgtgcgattt caatgttcgc ggcgcagtcg cttcgcagcc
     1681 cgcagtcgcc tagaaataga caagaatacg tccatgacaa cttccgcgac aggcgcctgg
     1741 gcgctcgagc gcgatgccga gggcatcgca tggctcacct tcgacaagcc cggaagctcc
     1801 acgaatgtcc tttcgcgtga aaccctggtg gaactcgatt cgcatctcga atcgttcaac
     1861 ggatcaccgc cgcgcggcgt agtcatccgc tcggcgaaaa aatccggctt catcgccggt
     1921 gccgacgtgc gcgagttcac gcagctcacg agtgcggaac aggccttcga gctggtgcgc
     1981 gcggcgcaac gaattctcga tcacctcgaa tcgctgccct gcccgaccgt ggccatcatt
     2041 catggctttg cgctcggcgg tggactcgag ctcgcgctcg cctgccgcta tcgcgtcggc
     2101 atccgcagcg accatttttc cattggcctg cctgaagtga tgctcggcat acatccgggt
     2161 tttggcggca cggtgcgcgc cgtgcgtcgt gcaggcgtac gcacggcgat ggagatgatg
     2221 ctgaccggca aaacgctgcg cgccgacagg gccaggaacg caggcttcct cgaccggttg
     2281 gtgtatccgg cggatgccga ggctgccgcc cgcgagctga tcaatcgcca gcctgcgcaa
     2341 cggcggccgc ggctgctcga tcgcatgctg tcatggccgc tggtccgcgg cctggtgaag
     2401 aagcagctca cggcccaggt gcgcgccaag gcgcggcctg agcactaccc tgctccctac
     2461 gccatcatcg acctctggtc gacgcatgga gcccgtggcg ccgccgcgta cgaagccgaa
     2521 gcgcgttcca tcgcgcagct gatgctgagc gacacctctc gcaatctggt acgggttttc
     2581 ctgctccagg accggctcaa gaatctgggt ggcaaatcga aggtcccact cgcacgcgtg
     2641 cacgtggtgg gcgcgggagt catgggcggc gacatcgccg cctggtgcgc acagcgcggc
     2701 ctcaacgtga cgctgcagga tcgcgagctg aaattcatcg agccggcgct cacgcgcgca
     2761 cgcgagggat ttgcaaagcg cctcaaggat aaagacaagg ccgcggtgct gatgacccgc
     2821 atgaccgcgg acgtcgccgg tgatggcgtg cccggggcgg acgtcgtcat cgaagccatc
     2881 ttcgagaatg ccgaggcgaa acatgagttg ttcggacggc tcgagccgcg catgaaaacg
     2941 agcgccatcc tcgcgtcgaa tacttcatcg atcatgctcg agcagctcga cgaccggctc
     3001 ccggatccgg ccaggctcgt cggcctgcac ttcttcaacc cggtggcgca gatgccgctg
     3061 gtggagatcg tgcgcggcga gcacagcagt gaagaatcgg tgcagaaggc catcgcgttc
     3121 gcgcgcaaga tcgacaagct gccgctgccc tgcaagagcg cgccggggtt tctcgtcaat
     3181 cgcgtgctgg tgccttacct gtacgaagcc atgttcgcgc tgcaggacgg cattccgatc
     3241 gaagtcatcg atgaagccgc gcttcgctac ggcatgccca tgggtccggt cgaactagcc
     3301 gacgtagttg gtctcgacgt gtgcaaacac gtgggtgaca tcgtgtcgaa ggcgctcggg
     3361 aagcagcagc ccgatactgc gcgcatcgat gcgctgatcg ccgcgaaaaa actcggtcgc
     3421 aagtcgggcg aaggttttta cgtctggcgt gatggcaagg cggtcaagtc gaagggcccg
     3481 catgctgaac ctccagccga tctcgaagat cgcctggtgc tcgcgctcgc caacgaatgc
     3541 gcggccgtat tgcgcgaggg aatagtcgcc gatgctgatc tcatcgacgc gggcgtgatc
     3601 ttcggctcgg ggttcgcgcc gtttcgcggt ggtccgctta cctacgcgcg caaacgcggc
     3661 gtcgaagtcg tggtcgcgcg cctcgtggaa ctcaccaaaa cgtacggatc tcgcttcacg
     3721 cccgacgcag ggtggacttc cttgagcacg gtcccttaag ccgcctcgct ggcgtgctag
     3781 catttcggca tgtcggatgc ccgtgaagtc agcgtagaac tgctgcgaac cttgaccccg
     3841 ctcgaaggaa tgaagcggga aaacctgcat gcgctggcca agaaggtcac cgtcaagcag
     3901 atgaatgccg gccgcctgct cttcaaggaa ggtgacgccg acaagcgcac catctggatc
     3961 atcaagggcc tggtggagat ttccgaacag ggccgcacca cggccatgat cgaaggcgga
     4021 agcgccgatg cgcgtgcgcc gctgaaccag agcgtaccgc gcaaggtcac tgcccgcgcc
     4081 gtcgaagatg tggattacct cgccatcgac gccgatctgc tcgacgtcat gatcacctgg
     4141 gatcagaccg gcaactacga agtcggcgag ctgcaggcgc agttcggagg cggcaatgaa
     4201 gacagcgact ggatgaccac gctgctgcag accaaagcgt tccaccgcat tccgcctgcg
     4261 aacctgcagg ccatcttcat gcgcatgcaa cgaaccccct atcgcgccgg cgaagtggtc
     4321 atcaagcagg gcgacgaagg cgactatttc tacgctatcg tccagggccg ctgcctggtt
     4381 acacgcgaat ctccgctcaa tcagaaaggc atcaagctcg cggagctcgg tgtcggtgaa
     4441 acctttggtg aagaggcgct gatcgccgaa gccaagcgca acgccacggt cagcatgctg
     4501 accgaggggg tgctcatgcg gctcaacaag caggacttcc gcgacctcat gcacgagccg
     4561 ctgctgcgct gggtcgacga agctcaggcg ctcaagatca tccagaaccg cggtcagtgg
     4621 ctcgacgtgc gcctaccgtc ggaataccag aacctggcca tcgaaggctc catcaatatc
     4681 ccgctgtatt tcgtgcgcct gaagttgaac gccctcgatc gcaagacgcc ttacgtggtg
     4741 gtttgtgaca ccgggcggcg cagttcggct gcggcgttca tcctttccga gcgcggcttc
     4801 gattcctacg ttctgaaggg cggcatcacg acgtcgaatt tgccgatgcg acgcccgggc
     4861 ggctgacgcc cgaaacctct gcccacaacg cacgttgtct cgtgcgcttg ttgcggttac
     4921 gcgcaggcgt gggtgccggc gcctggtagc gcaggatctc gatccatccg ccagacgcgc
     4981 gttcaaagat gaccttgaat cgcgcgtcat tgcctccgaa ggccttgccc cagctcacct
     5041 gcgcataatt gccgtcgctg acgatttcgc agtgctcgcg cggcccgggg tgggcacgcg
     5101 cgcgcaactc caccgccaga cccgtatcga tctcgccgtg caaagatgcg ttcttcagcg
     5161 agggacgcag gctgcggcga ggttcgccgt gagcataggc cagcgccgcg aaaggtccct
     5221 tggggacgac ctgcgctttc ggcagcgact cgacgatttc gaacggcccg cccgactccg
     5281 acacgaccat cacatggccg ccctcttcga ccgacaccgc gcatccgcgc atggtttcga
     5341 tcgtgagtgt ttcgacctgc tgaccggcgc tgtacgggcc gtggaaggac cgaaccactt
     5401 ccaccagcgc gcgacgcgcg tgtaacacgc cgaacgcgga tcgcgaatcc tgcggctcat
     5461 gcacttcgac tacataggct tcgaacgcgg catgcggtgc atagatgaga gtgccgggag
     5521 cgcgcggtgc cggcacgcag gggctcgccg cggcggcgag tgccggaaag gttgtcagga
     5581 gcatgaggca catgacagag cgcatgacca aatcgtcaca tgggtggtgg cgctttagtg
     5641 ggcaacggat catcaaattc cgtcactcga ctgcgaccga tgtcacgaaa aacgctggcg
     5701 gcggataaac cttaagaatc tggaaaatga cggcacaaca aacgtgaact gcgtctggta
     5761 gctagagtgc gaggaacttc acgctctgcg gtggatggtt acccgagccc tggggcccgg
     5821 ctctacatcg tgtgcgtggc tttgtcgccg gcgagcgcgc cggccaggta ggtctcgatc
     5881 ttcttctggg tctgtccgcg atcctggtcg gagaactgca cgccgatgcc ggcggtgcgc
     5941 ttgccctgcg cgcctttcgg cgtcagccag attacgcgac cggccacggg cagcttttcg
     6001 tcttcgccca tgaggttcaa cagcatgaac acctcgtctc cgaggcggta attgctgttg
     6061 gtggggatga acaggccgcc attcttcacg aacggcatgt aggccagata cagcgcgctc
     6121 ttgtccttga tggtgagcgt caggagtccg ggcttgttgg cgccggtgcg gcccgtgcgt
     6181 tcagtcatgg acgtgcccac cgccatagca gtgcttcaac agccatggtc ttgttgattg
     6241 aggtgtgggc aagcttgcgc atgtcgcgaa tggcgtccga aaactcgaat aggcgacata
     6301 tcttgggtgc caatgccgcc cctgacaagt gcgttacgtc gcggattgcg gcggattcga
     6361 ggatgcgatc tgtgacccag ctctcaaggc acgagaggcg tatcgaaagc tcgcctttcg
     6421 cccagcgttc ggccacggcg ggaggctgaa gattgccgct gccgatgtcc tgcagcgtgg
     6481 aaatcgtatc ggtgcgcagc tgcgcgagtg cggccgggtc tgcatccagc agcgtaaaag
     6541 gtccagcgcc agtggcggcc aacgcctcgg cccacggccc tgccccgcga gccgcttcga
     6601 gataattcgc cgcgacttca cgcgtgggcc ccgcaagtcg caaccgcgaa cagcggcttc
     6661 ggaccgtcgg caacagccgc gatggctggc tggttaccag caacaagagc gtgcgtttcg
     6721 cgggctcctc gagcgtcttg agcagggatt gcgcggcatt cggatggagc gcatcggccg
     6781 gcgagaaaat ggccactttg tagccacggc cgtgcgcagt gagcgtcaga tcggaggtga
     6841 gctcgcgtac agcctcgatc ttgatgtact gcgaatcttc ctcgggcgac agccgcgtca
     6901 cgtccggatg ctggtcggct gcgatccagc ggcactcctg gcattcgccg caaggcgcgc
     6961 gtgaggattc gcggcaattg acccgctgag cgatccagtg cgcgagttgc atcccgccag
     7021 cgcccgggtc ttcgtggatc agcagtccat gcgcgagccg gtccgcggca atcgccgcgt
     7081 tcacctcgcc gaacggcgtg gcgaaccagg atggcagcgg caacgtgctc acgagaccgc
     7141 ggcgggcggc agtgccaggc actcgtcgag tgccgcgatg gcagcggcgc agacctgatc
     7201 gagcttcgcc gtcgcatcga tgacacggaa acgagcggga aaggcgcgcg cgagttcgag
     7261 gtaccgattg cgcacgcggt cgaagaactg cgaagactcg atttcgaagc gatcgaccgc
     7321 gccgcgccgc gcctgcatgc gctcgagacc gactcgtacc ggtacgtcga gcaggatggt
     7381 gcaatcggga ttcagatccc cgtgtaccgc atgcgcgatc tggtcgatga gtttgcggtc
     7441 gacgccgcgg ccaccgccct ggtaggcgtg cgtcgcgtcg gtgaagcgat cacacagcac
     7501 ccactcgccg cgcgtcagcg ccggccggat gagattgtcg acgtgaacct ggcgcgcgcc
     7561 gaacatgagc agcgtctccg ccgtcgcgct gatgtgttcc tcgccgcgct cgagcaccag
     7621 cgcacgcacg cgttccgcca gcttggttcc gcccggttcg cgcgtcgcat gaatcgtgat
     7681 gccgcgcgcg cgcagcgact gcgtgagccg atcggcgacg gtggtcttgc cagcgccctc
     7741 gattccttcg agcgtgatga atttcgccgg catcatctgg gtacgcttgc tcctctcagt
     7801 cgcgcaagat accgcgcgac cgcggcattg tgttcctcga gcgtagcgga aaattcgtgc
     7861 gacccgtcgc cgattccagt ggccacgaag aagatgtcgc cggtttccag cgggcgtgtc
     7921 accgcttcga tggcttcccg ggatggaagc gcgatcggtg tcggcggcaa tccgccgcga
     7981 gtgtaggtgt tgtacggcgt gtcggtgcgc aggtcccgct cgcggatgtt tccgtcgtag
     8041 gaaggcccga gcccgtagat caccgtgggg tcggtctgca gtcgcatgtt tcgccgcagt
     8101 cgcgtggtga acacaccggc gatacgtggc cgttcgctcg cgagcccggt ctccttctcc
     8161 acgatcgagg cgagtgtcag agcttcatag gcgttcgcga gcggcagcgc ggcggcgcgt
     8221 gcgctccatg ccgcttcgag cgtctcggcc atcttgcgat acgccagcgc gtacaggtcg
     8281 cgatcggtag ttccgccggc gaagcgataa gtgtcgggaa agaagcgccc ttccggatgc
     8341 tcgcccgcgt gcccgatggc gctcatgatc tcggcgattt gcctgccgcg catcgtcacc
     8401 ttgatctgcg catgcgcttc gaccgcccgc cgcatgtctg caaatgtcca gccttcgatg
     8461 acggtgagtg tttcgagcac cacgcgccct tcggacagct ggcgcaggat ttcccggggg
     8521 ctggccagcg ccggaatttc gtagtgaccg gtcttgatct gcggccatcc acgcacacgc
     8581 atgaccagtt cgaccgcccg ccggtcggcc agggcgccgc gagtctcgag ttcggcgagc
     8641 gccgcgcgca cggtcgcgcc cggtttcacc aacagggcaa ccggttgctg atgtggaccc
     8701 ggcgcgtcca tgaccgcatt cgcgcgctgc cagtagatag tggctgccgc accggcgatc
     8761 acgccgaggc ccagaagaat caggagcagc ttacgcatcg agcggctcga tgtgcgccgc
     8821 gaggcgcttt gccaggttgt tcatgtcgta gagatgctct cctacgcgtc gcacgggcac
     8881 aaccccgata cgcgcattgg tgatgaaaac gccggcggcg gaaaacaggt cgtcgagtgt
     8941 caggcgctcc tcggcggcgg ccagcccgag cgcggcgcat tcacggagca cgatgccgcg
     9001 catcacaccg gcgacgccgc agctttgaat gagtggagtc gtcacgcagc catcgcgcac
     9061 gatgaagacg ttgctcattg cccccgagac caggtttccc gacgcatcga gcaacaggga
     9121 atcgaatacg ccccgggaag ccgattccgc ggcggcgagc acattctcca gacggttgag
     9181 atgtttgatg ccggcgagcg ccgggttctc gcccagcctg atggatgcca cgcacagagt
     9241 gacaccatca acgagcgccg cgtcccgcgg cggcgcgcgc cacaaggaaa cgaggcgtcg
     9301 cggtatttca tcgccttgcg gcgcgtaccc gcgccgctgc gcactgcctc gcgtcacgat
     9361 gatcttgaga accgcgagcg gcggggccac cgccaccgcc gcatcgatct ccgcgcgaag
     9421 catcgccagg ga
//
LOCUS       DASQJS010000286         3833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4251986, whole genome shotgun sequence.
ACCESSION   DASQJS010000286 DASQJS010000000
VERSION     DASQJS010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4251986"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1406
                     /locus_tag="VEW08_11445"
     CDS             315..1406
                     /locus_tag="VEW08_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HYJ41399.1"
                     /translation="MTEIAPLWALSPARFDRPDILKKLTAASRRLAELKGVAASIPNQ
                     GILINTLGLQEAKDSSAIENIVTTHDELFRDAAFPEDASSPATKEVAHYSLALHIGFE
                     DVRKSGLLTNKHILKIQSELERNSAGFRKLPGTALKASDGRTVYTPPQDPATIVALMT
                     DLEKFINENEAFDADPLIKMTLIHHQFESIHPFYDGNGRTGRIINVLYLVKQGLLDIP
                     VLYLSRHIVRTKSDYYRLLQEVRERGAWEDWVLYMLEAVEQTAAAGIETIRAIKALFL
                     DYKHRIRAQFKFYSQDLINNLFSHPYTKIEFIQKDLEVSRLTATKYLDQLTEGGFVQK
                     QKIGRSNYYINKPLYGILTGEAMRAGESQ"
     gene            1424..2473
                     /gene="dusA"
                     /locus_tag="VEW08_11450"
     CDS             1424..2473
                     /gene="dusA"
                     /locus_tag="VEW08_11450"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:NF008774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HYJ41400.1"
                     /translation="MKKAIDRRISVAPMMDWTDRHCRYYLRGFAPDTLLYTEMITTGA
                     VLRGDREKLLRFDPAEHPVALQLGGSDPGDLAASARIGADMGYDEINLNCGCPSDRVH
                     SGAFGACLMKKPSLVAECVDAMRTAVAIPVTVKLRIGVVDGAEIGGQGLSRRESAREA
                     AARFGAPERAALEEFARGVVAAGCAALIVHARKAVLGAWSPRDNREIPPLRYDVVRDL
                     QALVAPVPVILNGGLSTSAQVVSELAWADGVMLGREAYHRPMLLAELSAAGGEVPSRM
                     ELLERMTAYARRELARGERLSWITRHMLGLYSGLPGAKEFRRQLSEGARAPDAPAELL
                     LAAGEACERLSDAAA"
     gene            2550..2852
                     /locus_tag="VEW08_11455"
     CDS             2550..2852
                     /locus_tag="VEW08_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41401.1"
                     /translation="MKDVLPMIPGELSSDYRIRLALEQEHADERHRQAMSELTATANA
                     PDARIRAWERTHGLSLPKNSGHPVLNAVAAATRLTLEQVHAEQLRRVTPLPAATPA"
     gene            complement(2856..>3833)
                     /locus_tag="VEW08_11460"
     CDS             complement(2856..>3833)
                     /locus_tag="VEW08_11460"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYJ41402.1"
                     /translation="AGAVKDRIVFFANRMERTRDGSGYGKAVAVRAAGPSAASAAGAV
                     GVVIRSISTSDLRFPHTGATRYTTTAPRIPALAISNPDADALVRQFASGQPVRLSMRS
                     SSRDLPQMRSANVVGDIPGTDLAHEIVILGAHLDSWDPGVGALDDGAGVAIMMGVARL
                     IGELDVKPRRTIRVVLFANEEFGTSGSLAYLAANQADVSRHVLGFEADFGAGPVWRLS
                     SRVNPAQLPAVDQIFRALAPLKLVRGDNEARGGADLEGLSKLGMPILEPGLDGTDYFD
                     VHHTANDTLAQVDPKALRQSLAAFATTVWLGAQYSGTWERVAEPKPPRR"
BASE COUNT          751 a         1206 c         1205 g          671 t
ORIGIN      
        1 gaaagaatgc agaaacgtga agaactgaaa cacgggacac tgcgtatgcg tcgatttgcg
       61 acatttttgt cggctacgac cagttttgcg gagaaaagcg acatggcgtg cgtttacggt
      121 gctgagatca agtcggaggc gggggcgagc ccagcgcgcg gcctttacgc atctggtcgg
      181 gcacagcgaa ccgtccaggc aatccatgcg acggtgggtt gtgtacagga aggacaccga
      241 ccctttacag gtcgacatcg acatgtatat aaaacaggtg ttttctatgc acgtcactcc
      301 ttatctatat acaaatgact gaaatagccc ccctctgggc cttatccccg gcgcgcttcg
      361 accggccgga catcctgaag aagctgaccg cggcgagccg ccggctggcg gagctcaagg
      421 gcgtcgcggc gtcgattccg aaccagggaa tcctgatcaa tacccttggc ctccaggaag
      481 ccaaggacag ctcggccatc gagaacatcg tcaccaccca cgacgagctg tttcgtgacg
      541 ccgcatttcc cgaagatgcg tccagccccg ccaccaaaga agtggctcac tacagtctgg
      601 ccttgcacat cggtttcgag gacgtgcgaa aaagcggcct gctgactaac aagcacattc
      661 tgaaaatcca gtcggagctc gagcgcaaca gcgcaggctt tcgcaagctg cccggcaccg
      721 cgctgaaagc cagcgatggc cgcaccgtct acacgccgcc gcaggacccc gcgaccatcg
      781 ttgcgctgat gaccgatctc gagaagttca tcaacgagaa cgaagccttc gatgcagacc
      841 cgctgatcaa gatgacgctc atccatcacc agttcgagag cattcatccc ttctacgatg
      901 gcaatggccg cactgggcgc atcatcaacg tgctgtatct ggtgaagcag gggctgctcg
      961 atatccccgt gctctatctc agccggcaca tcgtgcgcac caagtccgac tactaccggc
     1021 tgctgcagga agtgcgcgag cgcggcgctt gggaagactg ggtgctgtac atgctcgaag
     1081 ccgtggagca gaccgctgcc gcgggcatcg agaccatccg ggctatcaag gctctgttcc
     1141 tcgactacaa acatcgaatt cgcgcgcagt tcaagttcta cagccaggac ttgatcaaca
     1201 acctgttctc acacccttac accaagattg agttcattca gaaagacctg gaagtgtcgc
     1261 ggctcaccgc gaccaaatac ctggaccaac tgactgaagg cggattcgtg cagaagcaga
     1321 agatcgggcg atccaactac tacatcaaca agcccttgta cggaattctc accggagaag
     1381 cgatgcgggc cggcgagagc caatgaaggt gacagagcac ccgttgaaaa aggcgatcga
     1441 tcgccgaatt tcggtcgccc cgatgatgga ttggacggac cggcattgcc ggtactacct
     1501 gcgcggcttc gcgcccgaca ctcttctata tacggagatg atcacgaccg gcgcggtact
     1561 tcgtggcgat cgcgagaaac tcctgcggtt cgatccggcg gaacacccag tcgcgcttca
     1621 gcttggcggc agcgacccag gagatctggc ggcttccgcg cggatcggcg ccgacatggg
     1681 ctacgacgag atcaacctca attgcggctg tcccagcgat cgtgtgcact ctggcgcctt
     1741 cggtgcctgc ctcatgaaga agccctcgct ggtggccgag tgcgtcgacg cgatgcgcac
     1801 cgccgtggcg atccccgtca ccgtgaaact tcgcatcggc gtcgtggatg gcgcggagat
     1861 tggcggccag gggctttcgc gccgcgagtc ggctcgcgag gcggcggcgc gcttcggggc
     1921 gccggaacgc gcggcgctgg aggaattcgc gcgtggcgtc gttgctgccg gatgtgccgc
     1981 actcatcgtg cacgcgcgca aggcggtgct cggcgcctgg tcgccacgcg acaaccgcga
     2041 gattccgcct cttcgttatg acgtggtacg tgaccttcag gcactcgtcg cgcccgtgcc
     2101 cgtgatcctc aatggaggcc tgagcacttc cgcacaggta gttagcgagc tggcctgggc
     2161 cgatggcgtg atgctgggcc gggaggctta tcaccggccg atgctgctgg cggagttgtc
     2221 ggccgcaggt ggcgaagtcc cctcacgcat ggagctgctc gagcgaatga cggcctacgc
     2281 tcgccgggag ctggcgcgcg gcgaacgcct gagctggatc acgcggcaca tgctggggct
     2341 gtattcggga ctgccgggcg ccaaggagtt tcgccggcag ctctccgaag gcgcgcgtgc
     2401 gccggatgcg cccgctgagc tgttgctggc ggctggggaa gcgtgcgaac ggctcagcga
     2461 cgccgcggct tgagcgaatc tcaatcgctt gagcaatttg tcccgcaagc gcgcagactg
     2521 ccgcgcaccc attacccgga gttcctgcat tgaaagacgt actgccgatg atccctggcg
     2581 agttgagttc ggactatcgc atccgcctcg cactcgagca ggagcacgcg gacgagcgcc
     2641 atcgacaggc aatgtcggaa ctgacggcga ccgccaatgc gcccgacgcc cgcattcgcg
     2701 cctgggaacg gactcacggg ctcagcctgc cgaaaaactc cggtcatccg gtgttgaacg
     2761 ccgtcgccgc cgcgactcgc ctgactctcg aacaggtcca cgcggaacag cttcgacggg
     2821 tcacgccatt gccggcggca actccggcct gatcctcatc gacgcggagg ctttggctcc
     2881 gctacgcgtt cccacgttcc ggagtactgg gcgcccaacc acacggtggt ggcgaaagcc
     2941 gccagcgact ggcgcagagc cttcgggtca acctgcgcca gggtgtcatt ggccgtgtgg
     3001 tggacgtcga agtaatcggt gccatcgagc ccgggttcga gaatgggcat gccgagcttg
     3061 ctcagacctt cgagatcggc gccgccgcgg gcttcgttgt cgccacgcac cagcttgagc
     3121 ggtgcgagcg cgcgaaaaat ctggtcgacg gccggcaatt gcgccgggtt cacgcgacta
     3181 gataggcgcc agaccgggcc ggcgccaaaa tccgcttcga agccaagaac gtggcgggaa
     3241 acgtcggcct ggttcgccgc caagtaagcc agcgagcccg aggtaccaaa ctcctcgttg
     3301 gcgaacagga ccacgcgtat ggttcgccgg ggtttgacgt cgagctcgcc gatgagtctg
     3361 gcgacgccca tcatgatggc gacgccagcg ccgtcatcca gcgctccgac tcccggatcc
     3421 caggaatcga ggtgcgcgcc gagaatcacg atttcatggg cgaggtcggt gcccggaatg
     3481 tcgccgacca cgttggccga gcgcatctgc ggcaggtcgc gcgagctgct tctcatcgac
     3541 aagcgtacgg gctggccgct tgcgaattgc cgtaccagcg cgtcggcatc cggattggat
     3601 atcgccagcg cgggaatacg cggtgcagtg gtcgtgtatc gggtcgcgcc agtatgtgga
     3661 aacctcaagt cgctcgtgct gatagaccgg atcaccacgc ccacggcgcc cgccgccgag
     3721 gcggctgacg gaccggcggc acgcacggca acggccttgc cgtatcccga gccatcgcgg
     3781 gtgcgttcca ttcggttggc gaagaaaacg atcctgtcct tgaccgcacc agc
//
LOCUS       DASQJS010000287        11339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1425305, whole genome shotgun sequence.
ACCESSION   DASQJS010000287 DASQJS010000000
VERSION     DASQJS010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11339
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425305"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VEW08_11465"
     CDS             complement(<1..479)
                     /locus_tag="VEW08_11465"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYJ41403.1"
                     /translation="MKTPPLALYAHFPWCVQKCPYCDFNSYTLRDELPEQRYVEALLR
                     DLDAQLVDLDARPLTSIFLGGGTPSLFAPAAIGRVIEHARTRMGFAGEIEITLEANPG
                     TIERGRFAEYRAAGVTRVSLGAQSFDERQLKLLGRIHSADETRRAAEELHAAGLSNF"
     gene            complement(476..1084)
                     /gene="rdgB"
                     /locus_tag="VEW08_11470"
     CDS             complement(476..1084)
                     /gene="rdgB"
                     /locus_tag="VEW08_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HYJ41404.1"
                     /translation="MRGQRVVLATGNPGKLRQFSELLGSSGFALVRQSDFGIEPPPET
                     GSTFLENSLLKARNAARLTGLPAIADDSGIEVDALNGRPGLFSARYAGEGASDEQNLR
                     KLLLELENVPPAKRTARYRSIIVFLTSADDPAPLIGEGVWEGSIATTPRGTGGFGYDP
                     IFIPRGHKRTAAEMPGEVRNKQSHRGQASRSFLAQLGERVRG"
     gene            complement(1106..1831)
                     /gene="rph"
                     /locus_tag="VEW08_11475"
     CDS             complement(1106..1831)
                     /gene="rph"
                     /locus_tag="VEW08_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HYJ41405.1"
                     /translation="MASRPSGRAPDELRAIKFTRRFTQHAEGAVLVEFGNTRVLCTAS
                     VEESVPGFLRGKNQGWVTAEYGMLPRATHTRSPREAAKGKQSGRTQEIQRLIGRSLRA
                     ITNMAALGERTITLDCDVLQADGGTRTAAITGSYVALADACDMLLAKRLISSSPLHGQ
                     VAAVSVGICSGVPVLDLDYAEDSGAETDMNVVMNNGGGFIEVQGTAEGHAFRRHELDA
                     LLNLAADGIGRLFQMQTEVRTAP"
     gene            complement(1831..2580)
                     /locus_tag="VEW08_11480"
     CDS             complement(1831..2580)
                     /locus_tag="VEW08_11480"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HYJ41406.1"
                     /translation="MRIEYAEVSLLGCRSDNQDRVSVAVSEHSALLVVIDGMGGHSDG
                     AKAAETAIKSLVEGFWHTPQPILDPLGFLHLTLGRAHEEVAKLGANLPLELRPRATCA
                     VCLVQNGSAFWAHIGDSRVYLLRRGKVFKRTRDHSHVEFLLREGVITADQAVSHPMRN
                     FVECCLGGEPFLPEMSIGLREPLEANDILLVCSDGLWGGLDDGDIGAAFPPARAPLRD
                     ELVRLAERSVSVVGAGSDNTSAAVVRWIGNA"
     gene            2656..3519
                     /locus_tag="VEW08_11485"
     CDS             2656..3519
                     /locus_tag="VEW08_11485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HYJ41407.1"
                     /translation="MIASMTGFARRELAGSWGTLTCELRSVNHRYLEPGFRLPEELRP
                     LESDLRQLLSKNLKRGKVDCTVHLRSMQAAERELRIDEAALSRVAAAVNQVARTVPGS
                     TIDAVEVLRWPGVIETGSQDSEALLGAGRTVFQQTLDELGAMRLREGKRLGELIEQRC
                     AGLGALVTQVRARLPEIQARVRTRLHERVAELLAAVDRDRVEQEIVLQLQRLDVAEEL
                     DRLTGHIEETRRVMSGGEAAGRRLDFLMQELNREANTLASKSQDLDTTRLTVDMKVLI
                     EQMREQVQNVE"
     gene            3516..4148
                     /gene="gmk"
                     /locus_tag="VEW08_11490"
     CDS             3516..4148
                     /gene="gmk"
                     /locus_tag="VEW08_11490"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HYJ41408.1"
                     /translation="MSAAAARRRGSLFVIAAPSGAGKTSLVNEVKTRDPSLRVSVSHT
                     TRKQRPTEVPGRDYNFVSVEEFKRLREAGEFLEYAQVFDNFYGTGRAQVEALRNAGHD
                     VILEIDWQGAQQVRKAEPDCKSVFILPPSRAELESRLRNRKTDSNDVIERRLRDAVTD
                     MSHYAEFDYVIVNDEFETAVAQMLTILRGGGTEYGAKRPALAPRLADLLG"
     gene            4258..4452
                     /gene="rpoZ"
                     /locus_tag="VEW08_11495"
                     /pseudo
     CDS             4258..4452
                     /gene="rpoZ"
                     /locus_tag="VEW08_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923811.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            4645..6777
                     /locus_tag="VEW08_11500"
     CDS             4645..6777
                     /locus_tag="VEW08_11500"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HYJ41409.1"
                     /translation="MPGLRELINSIEAYLPPEQVERVRSAAEFGAEAHEGQKRLSGEP
                     YIAHPLAAAQILADLHLDPDTIIAAILHDVIEDTPISKEELSEKFGAPVAEIVDGVTK
                     LDQIQFKSRAEAQAESFRKMVLAMVRDLRVILVKLADRTHNMRTLDAMAPARRRAIAR
                     ETLDIYAPIAGRLGLYNMKLELEDLGFEAAFPHRYKVLDRALKKARGNQKQFLNKIAE
                     TMRVALAKADLKADVSTREKHLYSIYSKMLRKAAPLSEIVDVFGLRIVVDRADTCYRA
                     LGVVHAAYKPMPGRFKDYIAIPRVNGYQSLHTTLFGPNGIPIEVQIRTEDMHRVAEAG
                     IAAHWKYKVNETEGDQDARAREWLANLVDMQEGGSSEDFLESVKVDLFPDKVYVFTPK
                     GEILRLPRGATVVDFAYAVHTDVGNRCVAAKVDRRLTPLRTPLRNGQTVEIITAKGAM
                     PNPSWVNFVVTAKARAAVRHYLKGLRRQEAIELGARLINQALGEFRLSLEEVAADTLN
                     AAVVELGMHDIDELYEKVGLGERLAPLVARRLLPTKASEESFSGAPAPLAIAGTEGLL
                     VAYARCCFPIPDDPIFAFLSAGRGVVVHRENCVNVEDYRKHPENWLPVAWEATTDKVF
                     SSEIRVDVANKMGVLAAVAAAISSGDTNIERVAVEEKDADSSSLVFELRVRNREHLAR
                     VVKVIRRMPEVLKVTRTIASHGRGSGRD"
     gene            6815..7204
                     /locus_tag="VEW08_11505"
     CDS             6815..7204
                     /locus_tag="VEW08_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016803893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYJ41410.1"
                     /translation="MTTIRQAIHTDQAPKAIGPYSQAVRVGDTVYMAGQIPLDPVTMQ
                     MVEGDFEKEATRVFENIKAVIAASGGTFRQVVKVTIFLTDFANFAKVNELMAKYFTEP
                     YPARSTVAVAALPRNARVEIECTLHLG"
     gene            7217..9313
                     /gene="recG"
                     /locus_tag="VEW08_11510"
     CDS             7217..9313
                     /gene="recG"
                     /locus_tag="VEW08_11510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HYJ41411.1"
                     /translation="MATPAPQPASEQRPVASMRGVGPALAEKLQKLGVTQVQDLLFVL
                     PSRYEDRTQVSEIGSVRHGTRVVVEGEIQLTEVAYRRRRQLLCRITDGTGSLTLRFFY
                     FSGAQQANLARGARIRCYGEVRRGPLGLEIVHPEYRRIGAEAAPLEEVLTPIYPTTEG
                     VPQTRLRSLIEIALRETEGSGIRDWVPPAMLADLELPSLLVALSMMHRPPRGAHLGEL
                     LSGHHPAQRRLAFEELLAHQLSLRLLRQEIKSDPAWRLDDASGLEKRFLASLPFKLTA
                     AQTRALAEVDADLTDDRPMVRLVQGDVGCGKTVVAAAAAARAVGGGLQAALMAPTELL
                     AEQHAKNFERWFRPLEIPVALITGSLPARTRRSALEAVASGDIKLVVGTHALFQESME
                     FEKLGLVIVDEQHRFGVHQRLSLRDKGARGGRLPHQLIMTATPIPRTLAMTAYADLDV
                     SVIDELPPGRTPVKTVVLPEPRRDEIVQRIHRAVLEGRQAYWVCPLIDESEELRYQAA
                     EETAAALAEALPEVRVGLVHGRMPPREKERGMYAFKEGKIGLLVATTVIEVGVDVPNA
                     TLMVIENAERMGLAQLHQLRGRVGRGAHESHCVLLYGGKLSEMARARLAAIRETNDGF
                     VIARRDLELRGPGELLGTRQTGLAEMRVADLMRDSDLLPAVQAAAETLLKDNPQAVQG
                     LTRRWVGSGERYGRIG"
     gene            9402..10076
                     /locus_tag="VEW08_11515"
     CDS             9402..10076
                     /locus_tag="VEW08_11515"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYJ41412.1"
                     /translation="MAVQILNDMPDAAVHRGDLADFSVHAQEARRGTSWISGRAVFVA
                     ATIGVHLIAAAAFMSIRQERKASEPEPIMATMMESSTAEPEPPRDLTPPPVNVVYSLT
                     VPQELAFESEAPPEVIQAAISPTTPQFTPPPMVDVEYVRAPAPVYPAESKRKRERGTC
                     VLRVMVDSSGRPAQIQVERSSGYERLDIAARDAVEKALFRPYEVNGIAQPAQVLVPIE
                     FTRRPS"
     gene            10107..11039
                     /gene="ubiA"
                     /locus_tag="VEW08_11520"
     CDS             10107..11039
                     /gene="ubiA"
                     /locus_tag="VEW08_11520"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HYJ41413.1"
                     /translation="MANQSLTDLDLTPPPSNARLTYKRYGRRILDLIEVARLNRPIGI
                     WLLLWPVLWAVWIAGASHPNPKVLVIFVLGTIAMRSAGCVINDLADRDFDPFVKRTRD
                     RPIAARRLAPYEAIVWFLALIAAALALVLQLDPATIRMSVIGAALAVSYPFFKRFFPL
                     PQFYLGVAFSWGVPMAFMAQLGEIPRVAWIIFFAGIIWAAIYDTMYAMVDRDDDLKLG
                     LKSSAIVFGDMDTLIIGVLQALMLYALYLVGTSMEYGYWYNAGLVGAGVFFAYQQWRI
                     RERKPQSCFDAFLNNHYVGLSVFIGILLQYAFNP"
     gene            11047..>11339
                     /locus_tag="VEW08_11525"
     CDS             11047..>11339
                     /locus_tag="VEW08_11525"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HYJ41414.1"
                     /translation="MPAFPLRSIVVVGGGTTGWLAATTLAHVLGSSCPVRLVSLPGEP
                     ADAGAVVSVPSLHRLLRLLKIDEIALMRATDATYRLGAMFRDWAAAGDAYFNG"
BASE COUNT         2049 a         3774 c         3578 g         1938 t
ORIGIN      
        1 ttgaagttgg agagaccggc ggcgtgcaac tcttcggcgg cacggcgcgt ctcatcggcg
       61 gaatgaatgc gcccgagcaa tttgagctgc cgctcatcga aactctgcgc acccagcgag
      121 acccgcgtga ctccggccgc gcgatattcg gcgaaacggc cacgctcgat cgtgcccggg
      181 tttgcctcga gcgtaatttc gatctccccc gcgaatccca tgcgcgtccg cgcgtgttca
      241 atgacgcgac cgatggcagc gggcgcaaac agactgggcg ttccgccacc cagaaagatg
      301 ctggtcagcg gacgcgcgtc caggtcgacg agttgggcat cgagatcgcg gagcagggcc
      361 tcgacgtagc gctgctccgg aagctcatcg cgaagcgtgt acgaattgaa atcgcaatac
      421 gggcacttct gcacgcacca gggaaagtgc gcatacaacg cgagcggtgg cgttttcatc
      481 cgcgtacccg ctcgcccagt tgcgcgagga aagagcgcga ggcctggccg cgatggctct
      541 gcttgttgcg cacttctcca ggcatttccg ccgccgtccg cttgtggccg cgaggaatga
      601 atatcgggtc gtagccgaac ccgccggtgc cgcgcggcgt cgtggcgatg gaaccttccc
      661 agacaccttc cccgatcagc ggcgccggat cgtcagcgct ggtgaggaat acgatgatgc
      721 tgcgataacg cgcggtgcgc ttcgccggcg gcacgttttc cagttcgagg agcagcttgc
      781 gaagattctg ctcgtcgctc gcgccttcgc cggcgtagcg ggccgaaaac aaccccgggc
      841 ggccgttcag cgcgtcgact tcgattcccg aatcatccgc gatggcagga agtcccgtga
      901 gccgcgctgc attgcgcgcc ttcagcaggg agttctccag aaaggtgctg ccggtttcgg
      961 ggggcggttc gatgccgaaa tcggattggc gaaccagcgc gaatcccgac gaacctaaca
     1021 actctgaaaa ttggcgaagc ttgccgggat tgccggtggc gagcaccacg cgctgacccc
     1081 gcatgagcgt ttgcgtcggc caccgtcagg gagccgtccg cacctcggtc tgcatctgga
     1141 acaacctccc gattccgtcg gcggcgagat tgagcagcgc gtccagttca tggcgacgaa
     1201 atgcgtgtcc ctccgccgtt ccctgcactt cgataaagcc gccaccgttg ttcatgacga
     1261 cgttcatgtc ggtctcggcg cccgaatcct ctgcgtagtc gagatcgagc accggcacgc
     1321 cggagcagat tcccaccgat accgcggcga cctgaccgtg tagcggcgaa gaagaaatga
     1381 ggcgtttcgc gagcaacatg tcacaggcat ccgccagcgc cacgtagctg ccggtgatgg
     1441 ccgcggtacg cgtgcctcca tcggcctgaa gcacgtcgca gtcgagcgtg atcgtccgtt
     1501 cgcccaacgc ggccatgttc gtgatggcgc gcagactgcg gccgatgagc cgctggattt
     1561 cctgcgtgcg gccactctgc ttgcccttgg ccgcttcacg cggcgagcgc gtgtgtgtgg
     1621 cgcggggcaa catgccgtat tcggcagtga cccagccctg gttcttgccg cgcagaaaac
     1681 ccgggacgct ttcttcgacg ctcgccgtgc acagcacgcg cgtgttgccg aactcgacca
     1741 gcactgcgcc ttcggcgtgt tgagtgaaac ggcgcgtaaa cttgatggcg cgcagctcat
     1801 ccggtgcgcg tccgctgggc cggctggcca tcaggcgttt ccgatccagc gaacgaccgc
     1861 ggctgatgtg ttgtcgctgc cggcgccgac gacgctcacc gaacgttcgg caagccgcac
     1921 gagctcatcg cgcagcggcg cacgtgcggg cgggaatgcg gcgccaatgt cgccgtcatc
     1981 gagcccgccc cagagaccat ccgaacagac caggaggatg tcgttggcct cgagcggttc
     2041 gcgcagaccg atcgacatct cgggcaggaa cggctcgccg cccaggcagc attcgacgaa
     2101 attgcgcatc gggtgactca cggcctgatc cgccgtgatg acgccttcac gcagcaggaa
     2161 ctcgacatgg ctgtggtcgc gtgtgcgttt gaagacctta ccgcgacgca gaagatagac
     2221 gcggctgtcg ccgatgtgcg cccagaacgc actgccattc tgcaccaggc acacggcgca
     2281 cgtcgcgcgc ggccgcagct cgagtggcag attggcgcca agcttcgcga cttcctcgtg
     2341 cgcgcgaccg agcgtcagat gcaggaatcc gagcggatcg agaatgggct gtggcgtgtg
     2401 ccagaagccc tcgacgagcg acttgatggc cgtctcggcg gctttggcgc catcggaatg
     2461 acctcccatg ccgtcgatga cgacgagcag ggcggaatgc tccgagaccg cgaccgaaac
     2521 gcgatcctga ttgtcggaac gacaacctag caggctgact tcggcgtatt cgatgcgcaa
     2581 ggcgtggtgg gtggtctgat aaactgccgc gcactttacc gcaccccgct ccccttaagc
     2641 cgagaactta cacgcatgat cgccagcatg accggattcg ctcgacgcga gcttgcggga
     2701 agctggggga ccctcacctg cgagcttcgc agcgtgaatc atcgctacct ggagcccggg
     2761 tttcgcctgc ccgaagagct tcgcccgctc gaatcggact taaggcagct gctgagcaag
     2821 aacctcaagc gcggcaaggt cgactgcact gtgcacctgc gcagcatgca ggccgccgag
     2881 cgtgagctgc gcatcgacga agcggcactt tcgcgcgtgg ccgcggcggt gaaccaggtg
     2941 gcgcgcacgg tgccgggcag caccatcgat gccgtggagg tattgcgctg gcccggggtc
     3001 atcgagaccg gaagccagga ctcggaagcc ttgttaggcg cgggccgcac ggtgttccag
     3061 cagacactcg acgagctcgg tgcgatgcgc ctgcgcgaag gcaagcgcct gggcgaactc
     3121 atcgaacaac gttgcgcggg gctcggcgcg ctggtcaccc aggtgcgcgc tcgccttccg
     3181 gaaatccagg cccgcgtgcg cacgcgcctg catgagaggg tggcggaact gctcgccgcc
     3241 gtcgatcgcg accgcgtgga gcaggaaatc gtgctgcaac ttcagcgcct cgacgtcgcc
     3301 gaagagctcg accgcctcac cgggcacatc gaggaaacca gacgcgtgat gagcggcggc
     3361 gaggcggccg gcagacgcct cgatttcctc atgcaggaac tcaatcgcga ggcgaacacc
     3421 ctggcctcga aatcccagga tctggacacc actcgcctca cggtcgacat gaaagtgctg
     3481 atcgagcaga tgcgcgaaca ggtacagaac gtcgaatgag cgcagcagcg gccaggcggc
     3541 gcggctcgct gttcgtcatc gccgcaccgt cgggtgccgg caagacgagc ctggtcaatg
     3601 aagtaaagac ccgcgatccc tcgctgcgcg tgtccgtctc tcacaccacg cgcaagcagc
     3661 gccctaccga agtccccggc cgcgactaca acttcgtcag cgtcgaggaa ttcaaacgcc
     3721 tgcgagaggc cggcgaattt ctcgagtacg cccaggtatt cgacaacttc tatggcaccg
     3781 gacgtgcgca ggtcgaagcg ctgcgtaacg ccgggcacga tgtaatcctg gaaatcgact
     3841 ggcagggcgc gcagcaggtg cgcaaggcag aaccggactg caagtcggtc ttcatcctcc
     3901 cgccctcacg cgccgagctg gaatcccgac ttcgcaatcg caagaccgac agcaacgacg
     3961 tcatcgaaag gcgcctgcgg gacgcggtca ccgatatgtc gcattacgcc gagttcgact
     4021 acgtcatcgt caacgacgaa ttcgaaaccg ccgtcgccca aatgctcacg atcctgcgtg
     4081 gtgggggaac ggagtacggc gccaaaaggc cggccctcgc gccacggctg gcagacctgc
     4141 tcggctgaac ggcggagccg gccgcccgaa cccggccgcg ctggtcaaaa aacaggcaaa
     4201 tcccggcctg tttccagtag actttcatcc ccccgcagct cgttcccgaa aggcactatg
     4261 gcccgcatta ccgtcgaaga ttgtctcccg aacgtcgaaa acatctttca gctcgtgctg
     4321 ctggccgccc agcgtgcgcg ccgcctcgcc aatggcgccg agcccaccgt tccctgggag
     4381 aacgacaagc ccaccgtggt cgccctgcgt gagatcgccg aaggcaacat cacgccagaa
     4441 atgctgcgcg agccggatcc cgcgccagtc gaagccccgc ttcctgacgc cgacaaccag
     4501 tccctgttcc gtgcgccgca attcggcctg caaggcgatc gtgacgtaca ggaataaatc
     4561 gtccgcctga tccttccgta aaggagctcg cgccccgtgg attacgcgtc gacgctcctt
     4621 cagcttctgc ccagtgcccg gcgcctgccg ggcctgcgcg agctgatcaa cagcatcgaa
     4681 gcctatctgc cgcccgaaca ggtggagcga gtccgctcgg ccgcggaatt cggcgcggaa
     4741 gcgcacgagg gccagaagcg gctctccggc gagccgtaca tcgcgcaccc gctcgcggcc
     4801 gcgcagatcc tcgccgacct gcacctcgac cccgacacga tcatcgcggc gattctgcac
     4861 gacgtcatcg aggacactcc gatctccaag gaggagctgt ccgagaagtt cggcgcaccg
     4921 gtggccgaga tcgtcgacgg tgtcaccaag ctcgaccaga tccagttcaa gtcgcgtgcc
     4981 gaggcccagg cggaatcttt ccgcaagatg gtgctcgcca tggtccgcga cctgcgcgtg
     5041 atcctcgtga agcttgccga ccgcactcac aacatgcgca cgctcgacgc catggcgccc
     5101 gcgcggcgcc gcgcgatcgc gcgcgagacg ctcgacatct acgcgccgat cgccggacgt
     5161 ctcggcctgt acaacatgaa gctcgagctc gaagaccttg gcttcgaggc ggcgtttcca
     5221 catcgttaca aggtgctgga ccgcgcgctc aagaaagcgc gcggcaacca gaagcagttc
     5281 ctcaacaaga ttgccgagac catgcgcgtg gcgctggcga aggcggacct gaaagccgac
     5341 gtcagcacgc gcgagaagca tctgtacagc atctacagca agatgctgcg caaggccgca
     5401 ccgctgtccg agatcgtgga cgtgttcggc ctgcgcatcg tggtagatag ggcggacacc
     5461 tgctaccgcg cgctaggcgt ggtgcatgcg gcttacaagc ccatgccggg gcgcttcaag
     5521 gattacatcg cgataccgcg cgtcaacggc taccagtcgt tgcacaccac gctgttcggt
     5581 ccgaatggca ttcccatcga agtgcagatc cgcaccgagg acatgcatcg cgtcgccgag
     5641 gcgggcatcg ctgcacattg gaaatacaag gtcaacgaga ccgaaggcga ccaggatgcg
     5701 cgcgcgcgcg aatggctcgc gaacctggtc gacatgcagg aaggcggcag ttccgaggat
     5761 ttcctcgaga gcgtcaaggt cgacctgttc cccgacaagg tctacgtgtt cacgcccaag
     5821 ggcgaaatcc tgcgcctgcc gcgcggcgcc accgtggtgg acttcgcgta tgccgtgcac
     5881 accgacgtcg gcaaccgctg cgtcgccgcg aaggtggacc ggcgcctgac gccgctgcgc
     5941 acgccgctgc gcaatggcca gaccgtggag atcatcaccg ccaagggcgc gatgccgaat
     6001 ccgtcgtggg tcaacttcgt ggtcaccgcg aaagcccgcg ccgccgtgcg ccactacctc
     6061 aaggggctgc gccgccagga ggccatcgag ctcggcgcgc gtctcatcaa ccaggcgctc
     6121 ggtgaattcc gactctcgct ggaagaggtc gccgcggaca cactcaatgc cgccgtcgtc
     6181 gagcttggca tgcacgacat cgacgagttg tacgaaaagg ttggcctcgg agaacgcctt
     6241 gcgccgctgg tggcacgccg gctgctgccc accaaggcca gcgaagagtc cttctccggc
     6301 gctccggcgc cgctcgccat cgcgggcacc gaaggtctgc tggtggccta cgcgcgctgc
     6361 tgtttcccga ttcccgacga tccgatcttc gccttcctct ccgcgggccg tggcgtggtc
     6421 gtccatcgcg agaactgcgt gaacgtcgag gactatcgca agcacccgga aaactggctg
     6481 ccggtcgcct gggaggcaac caccgacaag gtcttcagct cggagattcg cgtcgatgtg
     6541 gccaacaaaa tgggtgtact cgcggcagtc gctgccgcca tctcatcggg cgacacgaat
     6601 atcgagcgag tggcggtcga agagaaggat gccgacagtt cctcgctggt tttcgaattg
     6661 cgcgtgcgca accgtgaaca cctggcgcgc gtggtgaaag tgatccgccg tatgcccgaa
     6721 gtgctcaagg tcacgcgtac aatcgccagc cacggtcgcg gcagcgggcg cgactaacac
     6781 gcacaggaga gttcgcgatg gtcaggattt cgctatgaca accattcgtc aggcaattca
     6841 tacggatcag gcaccgaagg ccatcgggcc gtactcgcag gcggttcgcg tcggcgacac
     6901 cgtgtacatg gccggccaga tcccgctcga tccggtcacc atgcagatgg tggagggcga
     6961 tttcgagaaa gaagcgacgc gcgttttcga gaacatcaag gcggtcatcg ccgcttccgg
     7021 cggcacgttc cggcaggtcg tgaaggtcac catcttcctc acggatttcg cgaacttcgc
     7081 caaggtcaac gagctcatgg cgaagtactt caccgaaccc tatccggcgc gctcgacggt
     7141 cgcagtcgcg gcgctgccgc gcaacgctcg cgtcgagatc gagtgcacgc tgcacctggg
     7201 ttgatacgcc gttaaggtgg caacacccgc gccacagccc gccagtgaac agcggccggt
     7261 cgcgtccatg cgcggtgtcg gccctgcgct cgcggagaaa ctgcagaagc tcggtgtcac
     7321 gcaggtccag gacctgttgt tcgtgcttcc gtcccgttac gaagaccgca cgcaggtttc
     7381 cgagatcggc agcgtgcgtc atggcacacg cgtggtggtc gaaggcgaaa tccagctcac
     7441 cgaagtggct tatcgccgcc gccgccagct actgtgccgc atcaccgatg gcaccggctc
     7501 gctgacgctg cgcttcttct acttctccgg tgcgcagcag gccaatcttg cccggggcgc
     7561 gcgcatccgc tgttacggcg aggtgcgccg cggccctctg ggcctcgaaa tcgtccaccc
     7621 cgaataccgc cgcatcgggg ccgaggcggc cccgctcgaa gaggtgctga cgcctatcta
     7681 cccgaccacc gaaggcgtgc cgcagacgcg gctgcgctcg ctcatcgaga tcgcgctgcg
     7741 cgagaccgaa ggttcaggca ttcgcgactg ggttccgccc gcgatgcttg ccgatctcga
     7801 attgccatca ttgctcgtag cgctatcgat gatgcatcgg ccgccgcgag gagctcacct
     7861 gggcgagctc ctgagcggtc atcatcccgc gcagcgccgc ctggccttcg aagagctgct
     7921 ggctcaccag ttgtcacttc gactgctgcg ccaggagatc aagagcgatc cggcctggcg
     7981 cctcgacgac gccagtgggc tcgagaaacg ttttcttgcc tcgctgccgt tcaaactcac
     8041 cgcggcgcag acccgcgcgc tcgcggaagt cgacgccgat ctcaccgacg accggccgat
     8101 ggtccggctg gtgcaaggag acgttggctg cggcaagacc gtggtggcgg ccgctgctgc
     8161 cgcgcgcgcc gtcggcggcg gactgcaggc cgccctcatg gcgcccaccg agttgctggc
     8221 ggaacagcac gcgaagaatt tcgagcgctg gttcaggccg ctcgagatac cggttgcatt
     8281 gatcaccggc tcgttgccgg ctcgcacgcg gcgcagcgcg ctcgaggccg tcgccagcgg
     8341 cgacatcaag ctggtggtgg gcacacacgc tttgttccag gaatccatgg agttcgagaa
     8401 gctgggcctc gtgatcgtcg atgagcagca tcgcttcggc gtacaccagc gcctctcgct
     8461 gcgcgacaag ggcgcgcgcg gcggcaggct tcctcaccag ctcatcatga cggcgacgcc
     8521 gatcccgcga acgctggcca tgacggctta cgcggacctc gacgtgtcgg tcatcgatga
     8581 actgccgcca ggccgcactc ccgtgaagac agtggtgctg cccgaaccgc gtcgcgacga
     8641 aatcgtgcag cgcattcaca gggccgtgct cgagggccgc caggcgtact gggtctgtcc
     8701 gctcatcgat gaatccgaag agctgcgtta ccaggcagcc gaagaaaccg cggccgcgct
     8761 cgccgaagct ctgcccgaag tgcgcgtcgg cctggttcac ggccgcatgc caccgcgcga
     8821 gaaggagcgc gggatgtacg cattcaagga aggaaagatc gggttgctag ttgcaaccac
     8881 cgtcatcgag gtgggtgtcg acgtgcccaa cgccacgctg atggtcatcg aaaacgccga
     8941 gcgcatggga ctcgcgcagt tgcaccagtt gcgcggccgc gtgggtcgcg gtgcgcacga
     9001 gagtcactgc gtgctgctgt acggcggcaa gctttccgag atggcgcggg cgcgcctcgc
     9061 cgccatccgc gaaaccaatg acggcttcgt gatcgcacgc cgtgacctgg agctgcgggg
     9121 tccgggcgag ctgctcggca cccggcaaac cggcctcgcc gaaatgcggg tggccgatct
     9181 gatgcgcgat tcggacctgc tgccggccgt gcaggccgct gccgaaacgc ttctgaagga
     9241 caatccccag gcagtacagg gacttacacg tcgatgggtg ggctccggcg agcgttatgg
     9301 ccgcatcggg tagccgcctg gccccggcgt gacccgcagc acatagataa gaatcggcgt
     9361 tgaggtccaa atgcgaatca tttacattcg taccccaacc catggccgtc cagatcctca
     9421 acgacatgcc cgacgctgcg gtccaccgcg gcgacctcgc ggatttctcc gttcacgctc
     9481 aagaggcgcg gcgcggaact tcttggatct cgggtcgcgc ggtattcgtg gccgccacga
     9541 ttggcgtgca cctcatcgct gctgccgcat tcatgagcat tcgccaggag cgcaaggcaa
     9601 gcgagcccga acccatcatg gccacgatga tggaatcttc gaccgccgaa cccgaaccgc
     9661 cacgcgatct caccccgcct cccgtcaatg tcgtgtactc gctgacggtg ccgcaggaat
     9721 tggctttcga gagcgaagcc ccgccggaag tcatccaggc ggccatctcg ccgacgacgc
     9781 cccagttcac cccgccaccc atggtcgatg tggaatacgt gcgcgcgcca gcacccgtgt
     9841 atccggctga atcgaagcgc aaacgcgagc gcggcacctg cgtgctgcgc gtgatggtcg
     9901 attcgagtgg ccggcccgcg cagatccagg tggaacgctc gagcggctac gaacgcctgg
     9961 acatcgccgc gcgcgacgcc gtggaaaaag cgctgttccg tccgtacgaa gtgaacggca
    10021 ttgcgcagcc cgcgcaggtt ctcgtcccca tcgagttcac gcgccgcccg agctgaactc
    10081 cagtcccgcg tatatgatgc gcgccagtgg ctaaccagtc cctcaccgac ctggatctca
    10141 ccccgccgcc gtcgaacgcg cggctgacgt acaagcgcta cggacggcgc attctcgatc
    10201 tcatcgaagt cgcgcgcctg aatcgcccca tcggcatctg gctgttgctc tggccggtgc
    10261 tatgggccgt gtggatcgcc ggcgcctcgc atccgaaccc caaagtgctg gtcatcttcg
    10321 tgctggggac gatcgccatg cgctcggcgg gttgcgtcat caacgatctg gccgatcgtg
    10381 acttcgatcc attcgtgaaa cgcactcgcg accggcccat cgccgcgcgc cggctcgcgc
    10441 cctacgaagc catcgtctgg tttctcgcgc tgatcgccgc cgcactcgcg ctggtgctgc
    10501 agctcgatcc ggccaccatc cgcatgtcgg tcattggcgc tgcgctcgcg gtgagttatc
    10561 cgttcttcaa gcgtttcttt ccgctgccgc agttctatct cggcgtggcc ttcagctggg
    10621 gcgtgcccat ggctttcatg gcgcagctcg gcgaaattcc gcgcgtggcg tggatcatct
    10681 tttttgccgg catcatctgg gccgccatct acgacaccat gtacgcgatg gtcgatcgcg
    10741 acgatgacct caagctcggc ttgaaatcga gcgccatcgt ctttggcgac atggacacgc
    10801 tgatcatcgg cgtgctgcag gcgctgatgc tctatgcgct gtacctcgtc ggcaccagca
    10861 tggaatacgg ctactggtac aacgccggcc tggtcggcgc gggcgtgttc ttcgcctatc
    10921 agcagtggcg catccgcgag cgcaagccgc agtcatgttt cgacgccttc ctcaacaatc
    10981 attacgtcgg gctttcggtc ttcatcggca tcctgcttca gtacgcattc aatccttaga
    11041 gattcagtgc cagcgtttcc gcttcgcagc atcgtggtcg ttggcggcgg caccacgggc
    11101 tggctcgccg cgacgacgct ggcgcacgtg ctcgggagtt cctgcccggt gcgcctggtc
    11161 tccctgcccg gagaacctgc ggatgcaggc gcggttgtca gcgttccaag cctgcaccgg
    11221 ctgctgcggc tgctgaaaat cgacgagatc gccttgatgc gcgcgaccga tgccacgtat
    11281 cggctcgggg cgatgttccg cgactgggcc gccgccggcg atgcgtattt caacggctt
//
LOCUS       DASQJS010000288         9831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3320553, whole genome shotgun sequence.
ACCESSION   DASQJS010000288 DASQJS010000000
VERSION     DASQJS010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9831
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3320553"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /gene="argS"
                     /locus_tag="VEW08_11530"
     CDS             <1..1547
                     /gene="argS"
                     /locus_tag="VEW08_11530"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HYJ41415.1"
                     /translation="LPKSELVSSAEVAGAGFINFRLAKDAWFTPLKSVASQGAGYGRS
                     NVGAGRKVMIEFVSANPTGPMHVGHGRGAAYGATLGNLLEATGHSTYREYYVNDAGRQ
                     IDILAISVYLRYLELCGEPIAFPSNGYRAEYILPVARALQAMRGEALKRPAVEITRGA
                     PLDAPAGDKDKHIDGLIENARRALGTDEFDSIVIFARDAMMADIRNDLEEFGVRFDNW
                     YSERDLSRSGAINRALDALRASGHVYVKDGATWFKSTDYGDDEDRVIIRENGVGTYFA
                     ADIAYHFDKRQRGHDLLIDVWGADHHGYVARVRGALMALGAPGDCFEVTLMQLVSLFR
                     GGEKLAMGKREGNFVTLRDLRNDVGNDACRFFYLLRSHDQALDFDLELAKSRSNENPV
                     YYIQYAHARVASVMKQLASKGHTYDAGAADLSLLTNVHEQAVLTAVSKYPEIIEHAAL
                     ARAPHALVHFLRDVANTLHTYYNAEQFIVEEAPLRNARLTLVLAVQQVIRNGLGILGV
                     SAPETM"
     gene            1553..2134
                     /locus_tag="VEW08_11535"
     CDS             1553..2134
                     /locus_tag="VEW08_11535"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYJ41416.1"
                     /translation="MAVKITSRDFKRARPGGLKFDVARWREFGVGLAVGLSLSLFVLI
                     WQNYREEQVATEVATIPKPEPRADKAAAGDAEEGAKNYDFYDMLPKFEVVVPEKEREV
                     ASDRGSAPANIERPGVYVLQAGSYRQQSEADRIRAQLKMQGIDANVQRVAVDEDVWHR
                     VRIGPITDLTELNRLRARLRTADFDALVIRVGD"
     gene            2139..3260
                     /locus_tag="VEW08_11540"
     CDS             2139..3260
                     /locus_tag="VEW08_11540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HYJ41417.1"
                     /translation="MVRTSRLRISIGAALLTVLAACATQPPAAPPAPVTPPPPAAPQV
                     PPIPPPTHAGLTLAAVGDMMLGTDFPENILPDDDGLSFFDAVTPILEKPDVTFGNLEG
                     VLQDGGEPVKQCKNKKICFLFRTPARYATYFKLAGFDVLSLANNHARDFGEEGRSSSM
                     AALDAAGIHHSGREGTVASFIANGRRIAMVAFAPNVGSNSLNDPQIGLPLVADLATKH
                     DIVIVSFHGGAEGNGAEVLPFAREIFAGEDRGNVVEFARAMVDAGADIVLGHGPHVVR
                     PMEIYRDRLIAYSLGNFATYYGISVEGIRGIAPILLVTLDDEGKFVSGRIEPTIQIRP
                     AGPAPDPAKGVIKLLQSLTTTAFPAAALSIGDDGSLSRH"
     gene            complement(3322..4689)
                     /locus_tag="VEW08_11545"
     CDS             complement(3322..4689)
                     /locus_tag="VEW08_11545"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYJ41418.1"
                     /translation="MSAAHILVVDDEADIRGLLKEILSEEGYDVDVAGDAGQARASRA
                     RQLPDLVLLDIWMPDTDGITLLREWQGAAAESSCPVVMMSGHGTVETAVEATRLGAFD
                     FVEKPLSLQKLLRTVERALEAGKRKRHAGKLLAASPGVPVGRSRVMQQLRADLAQIAT
                     NDSPVLLVGETGTGREAFARFIHDASPRAARPFVTLISSTLRGEGADQVLFGTESGGQ
                     IAPGVLERAGAGTLFINEIEDLPPQSQRALLGTMESGQFTRVGGSVPVPFKARMLSSS
                     KPGIESRGEEFRRDLLAHLNTLIVRVPPLREYTEDVPDLLRHYVDRMVDGEGLQFRKF
                     SVAAQNRLRNYPWPDNVRELKNLVQRLLLQGGGEEIRLEEIERELAAQAPSVEPLVKQ
                     DLLALPLREAREQFERAYLQQQLLLCNGKVGQLAKRVGMERTHLYRKLRSLGVDFRNI
                     TDETE"
     gene            complement(4686..6932)
                     /locus_tag="VEW08_11550"
     CDS             complement(4686..6932)
                     /locus_tag="VEW08_11550"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ41419.1"
                     /translation="MVPAALRRLGTLGLIALWVLVSGGLLLLLALSVQNSMQFSRLQA
                     WILLANVIALIAVSILLARKIIELVRAFRAGVPGSRLTARTVAIFGSLVIAPLIIVYL
                     FSLEFLNQGIDSWFKVEIKQGLTDALELSKSAVNVRMREYARSTELFAHRLADSPPSN
                     WAEATDVQRVETGALEVVVYGTLGQVLAASSSSATSTSLPRVSAEVASAIAAGNLYLS
                     LEPLSEDDYLINTAAPIASGPGSSQGRFVVAQYPVPPQLSRLSEAVQHSYSQHYNLTQ
                     QREGVKNLFGMTLTLVLLLAMLTAIYGAIYSAEKLVRPVQDLIAGTRAVGKGDFGTRL
                     PLPSRDEMGFLVHSFNDMTKRLRRAREENTRSQNAVERERERLSIILARLSTGVIAIG
                     HDLVLRSANQAASNILGANLEQGVGQPLAALSGENPRYERFVGEVTMRLARGEEEWRE
                     QLDLKPEAAGARVLVCACTPLPGDEHAEDDAAALGGYVIVFDDISALLQAQRDAAWGE
                     VARRLAHEIKNPLTPIQLSAERMRRRYLHQMSGEDAQILDRATYTIVQQVEAMKAMVN
                     AFSEYARAPDMKVTKFPLNALVTEVADLHRVQESGVRIDLDLDPRIDSVEADRGRVRQ
                     ILNNLIVNGAEAVENTPGGHVVAATRLESVGSAEYATITVTDNGPGFAREVLGRVFEP
                     YVTSKPKGTGLGLAIVKKIVEEHGGRVEADNRPEGGGRVRVSLPVTDGSRQLVTRDRR
                     AEPRKERA"
     gene            complement(6914..7501)
                     /locus_tag="VEW08_11555"
     CDS             complement(6914..7501)
                     /locus_tag="VEW08_11555"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HYJ41420.1"
                     /translation="MQFRANNLGLLLLAALLSVSPGGVVRADVLDGVLEVRSAYVKPE
                     QGVFQLFARVTYPVNDDIRAALKDGLMLNFDLDVVVSRERRFWVDETVAEYTLRRELL
                     YHAVSDRYVARDVDDRNLDQHSYATLEEALEALGTVDAWPFLLSPQLSTNRQYRVALR
                     AGVRRGRLSDTMRVLMFWTDDWHRESEWFSWSLQH"
     gene            complement(7522..8889)
                     /gene="rsmB"
                     /locus_tag="VEW08_11560"
     CDS             complement(7522..8889)
                     /gene="rsmB"
                     /locus_tag="VEW08_11560"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HYJ41421.1"
                     /translation="MKKVRRNPPPPPRRTQYAPGAETLAAAALAVHDVVFEGRSSDAA
                     LEAAHERQDRAAVRAITLGTLRWYLRLKPALTPLLNRPFDELSPRLAALLVTTAHQVE
                     YSRGAAEAQVHLGVDASRVLEEGRASGVVNAVLRRFVAERAQLLATADEKLSQRHAHP
                     QWLVEALNTAWSERAPEILDANNQHPPMVLRLDPALTSAEDFLRSLRAIGRDATAVEW
                     NRDAMVLDRPAPVQVLPGFETGAVSVQDCGAQLAAPLLDAQPGMRVLDACAAPGGKTL
                     HIAQRTAGLAELIAVDDDALRLARVRENLERAGRDAILVKADLRTAPPSLQLAHFDRV
                     LVDAPCSATGVIRRHPDIKLLRRPADIESFAATQRQILATAFELLRPGGRLIYCTCSV
                     MPVENEGVVAEFLDAFPRAALAPWPEGLARPPGLLDRTVGWQLLPGGIAGTDGFYYAC
                     LTKLS"
     gene            complement(8886..>9831)
                     /gene="fmt"
                     /locus_tag="VEW08_11565"
     CDS             complement(8886..>9831)
                     /gene="fmt"
                     /locus_tag="VEW08_11565"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HYJ41422.1"
                     /translation="PMTSFRIAFAGTPQFALPALRALLKSTHQVVGVLTQPDRPAGRG
                     QHLRASPVKLLAAEHRLPIAQPSTLKTPEGHADLAQWAPDVLVVVAYGLILPQAALDL
                     PRLGCLNIHGSLLPRWRGAAPIQRAILAGDAETGVTIMQLDAGLDTGPMLLERSRSIG
                     LHDTAGDLHDALSELGAVALIEALDGLAAGTLRPRAQHATGASHAPKIEKSEAALDWS
                     ASAVQLDRQVRAFNPWPVAETCFGNETLRVLRARVAEPGGVHAEPGTLLGLADDGLRV
                     ACGEGVLAVCELQRAGKRPISARDFANAVRVAGLRFGS"
BASE COUNT         1694 a         3242 c         3249 g         1646 t
ORIGIN      
        1 cgctgccaaa aagcgaactg gtctcgagcg ccgaagtcgc cggcgccggt ttcatcaatt
       61 tccgcctcgc caaggacgcg tggttcacgc cgctcaagag cgtggcgtcg cagggcgccg
      121 gctacggccg cagcaacgtc ggcgccgggc gcaaggtgat gatcgagttc gtttcggcaa
      181 atcccacggg ccccatgcac gtcgggcacg gccgcggcgc cgcgtacggc gcgacgctgg
      241 gcaatctgct cgaagccacg ggacattcga cgtaccgcga gtactacgtc aacgatgcgg
      301 gccggcagat cgacatcctc gccatcagcg tctatctacg ttatctcgag ctgtgcggcg
      361 aaccgatcgc ctttccgtcg aacggttatc gcgccgaata catcctgccc gtggcgcgcg
      421 cgctgcaggc aatgcgtggc gaggcgctca agcgtccggc cgtcgaaatc acccgtggcg
      481 caccgctcga cgcgcccgcg ggcgacaagg acaaacacat cgacgggctc atcgaaaacg
      541 cccgccgcgc gttgggcacg gacgaattcg attccatcgt gatcttcgcg cgcgacgcca
      601 tgatggccga catccgcaat gacctcgaag agttcggcgt gcgcttcgat aactggtatt
      661 cggagcgcga tctgagccgc agcggagcca tcaatagggc cctcgacgcg ttgcgcgcaa
      721 gcggccacgt gtacgtgaag gacggagcca cctggttcaa gtccacggac tacggcgatg
      781 acgaagaccg cgtgatcatc cgagaaaacg gcgtcggcac ttatttcgct gcagacatcg
      841 cttaccactt cgacaagcgc cagcgcggcc acgacctgct gatcgacgtc tggggcgcgg
      901 atcaccacgg ctacgtcgcg cgcgtgcgcg gcgcgttgat ggcgctcggc gcaccgggtg
      961 actgcttcga agtcacgctc atgcagttgg tgagtctctt ccgcggcggt gaaaaactgg
     1021 ccatgggcaa gcgcgaaggc aacttcgtga cgctgcgtga cctgcgcaac gacgtcggca
     1081 acgacgcctg ccgcttcttt tacctgctgc gcagccacga ccaggcgctg gatttcgacc
     1141 tcgagctggc gaaatcccgc tccaacgaaa accccgtgta ctacatccag tacgcacacg
     1201 cgcgcgtggc gtcggtgatg aagcagcttg cctcgaaagg ccacacctac gacgccggcg
     1261 cagccgatct gtcgctgctc acgaacgtcc acgagcaggc ggtgctcacg gccgtgtcga
     1321 agtatccgga gatcatcgag cacgctgcgc tcgcgcgcgc accgcatgcg ctggtgcatt
     1381 tcctgcgcga tgttgcgaac acgctgcaca cctactacaa cgccgaacag ttcatcgtcg
     1441 aggaagcgcc cttgcgcaat gcgcgactga ccctggtcct ggcggtacag caggtgatcc
     1501 gcaacgggct cggcattctc ggcgtgtctg cacccgagac gatgtaaggc ccgtggccgt
     1561 caagatcacc agccgcgatt tcaaacgcgc ccgacccggc ggcctcaagt tcgacgtggc
     1621 gcgctggcgc gaattcggcg tcggtctcgc ggtcggcctg tcgctctcac tgttcgtgct
     1681 gatctggcag aactaccgtg aggagcaggt ggctaccgaa gtcgccacca taccgaagcc
     1741 cgagccgcgc gccgacaagg ccgccgccgg cgacgccgag gaaggcgcca agaactacga
     1801 cttctacgac atgctcccca agttcgaagt cgtggtgccc gagaaagaac gcgaggtcgc
     1861 cagcgatcgc ggcagcgcgc ccgcgaatat cgaacgcccc ggcgtctacg tgctgcaggc
     1921 cgggtcctat cgccagcagt ccgaggccga ccggattcgc gcgcaactca agatgcaggg
     1981 catcgacgcc aacgtgcaac gcgtggccgt ggacgaagac gtctggcacc gcgtgcgcat
     2041 cggccccatc accgatctca ccgagctcaa ccggttgcgc gcgcggctgc gcaccgcgga
     2101 tttcgacgcg ctggtcatcc gggtggggga ctagttagat ggtacggacg tcgcgcctgc
     2161 ggatctcgat cggcgcggct ttgctcacgg tgctggccgc ctgcgctacc cagccgccgg
     2221 ccgcaccacc ggcaccggtg actccaccgc ccccggccgc gccccaggtg ccgcccatcc
     2281 cgccaccgac gcacgctggc cttacgcttg cggccgtggg cgacatgatg ctgggcacgg
     2341 attttcccga gaacatcctg cccgatgacg acggcctgtc gttcttcgat gccgtgacgc
     2401 cgatcctcga aaagccggac gtgacgttcg gaaatctcga aggcgtgctg caggacggcg
     2461 gcgagccggt gaaacagtgc aagaacaaga agatctgctt cctgttccgc acgcccgcac
     2521 gctacgccac gtatttcaag ctggcgggtt tcgacgtgct gagcctcgcc aacaaccacg
     2581 cgcgtgattt tggcgaggaa ggccgttcgt ccagcatggc cgcgctcgat gcggcgggca
     2641 tccatcactc gggacgcgag ggcacggtcg ccagtttcat tgcgaacggc cggcgtatcg
     2701 ccatggtcgc ctttgcccca aacgtgggct cgaactcgct caacgaccca cagatcggcc
     2761 tgccgctggt ggcggatctc gcgacgaaac acgacatcgt catcgtgtcg tttcacggcg
     2821 gtgccgaagg caacggcgcc gaagtgctgc cgttcgcgcg cgagatcttc gcgggtgaag
     2881 atcgcggcaa cgtcgtggag ttcgcgcgcg ccatggtcga tgccggtgcc gacatcgtgc
     2941 tcggccacgg accgcacgta gtaaggccga tggagattta ccgggaccgc ctcatcgcgt
     3001 acagcctggg caacttcgcg acgtactacg gcatcagcgt cgaaggcatc cgcggaatcg
     3061 cgccaatcct gctggtcacg ctcgacgacg aaggcaagtt tgtctcgggg cgcatcgagc
     3121 cgacgatcca gatccgccct gccggtccgg cgcccgaccc ggcgaaggga gtcatcaagc
     3181 tgctgcagtc gctgacgacc actgcgttcc cggccgccgc gctctcgatt ggcgacgacg
     3241 gctcgttgtc acgacactag gcaagaccac ggttcccggt gctgcggcaa cgtctctacg
     3301 acggccgcgt gggcgcgcgt tctattccgt ctcgtccgtg atgttgcgga agtcgacgcc
     3361 gagagaccgc agcttgcggt acaggtgcgt gcgttccatg ccgacacgct tggccagctg
     3421 cccgaccttg ccgttgcaca gcaacagctg ctgctgcaga taggcgcgct cgaactgttc
     3481 gcgggcttcg cgcaacggca gtgcgagcag atcctgcttg accagcggct cgacgctcgg
     3541 cgcctgggcc gcgagctcgc gttcgatttc ctcgagccgg atttcttcgc cgccgccctg
     3601 caggagcagg cgctgcacga gattcttgag ctcgcgaacg ttgtctggcc aggggtaatt
     3661 acgcagccgg ttctgcgccg caacgctgaa cttgcggaac tgcaggcctt cgccatccac
     3721 catgcggtcg acgtagtggc gcaacaggtc gggtacgtct tcggtgtact cgcgcagcgg
     3781 cggtacacga acgatgaggg tattcagatg ggcgagcagg tcgcggcgga actcttcgcc
     3841 gcggctttcg atgcccggct tcgacgacga cagcatgcgc gccttgaacg gcacggggac
     3901 gcttccgccc acgcgcgtga actggccgct ctccatggtg ccaagcaatg cgcgttgcga
     3961 ctgcggcggc aggtcttcga tttcgttgat gaacaaggtg ccagcgccgg cacgttcgag
     4021 cacgccgggg gcgatctgcc cgccggattc ggtgccaaac agcacctggt cggcgccttc
     4081 gccgcgcagc gtgctggaaa tgagtgtgac gaacggccgc gcggcacgcg ggctggcatc
     4141 gtgaatgaat cgcgcgaagg cttcgcggcc ggtgccggtc tcgccgacca gcaacacggg
     4201 ggaatcgttg gtggcgatct gcgcaaggtc ggcgcgcaac tgctgcatga cacggctgcg
     4261 acccacgggc acacctggcg atgcggctaa caacttgccg gcatgacgct ttctcttgcc
     4321 agcctccagg gcgcgttcga cggtgcgcag gagcttctgc agcgacagcg gcttctcgac
     4381 gaagtcgaat gcgccgagac gcgtggcttc gaccgcggtt tccaccgtgc cgtgcccgga
     4441 catcatgacg acgggacagg atgactcggc ggccgcgccc tgccactcgc gcagcaaggt
     4501 gatgccgtcg gtatcgggca tccagatgtc gagcagcacg aggtcgggca gctggcgcgc
     4561 gcgcgaggcg cgggcctgcc cggcgtcgcc tgcgacgtcc acgtcataac cctcttcgga
     4621 aagtatttct ttcagaaggc cacgaatgtc tgcctcgtca tcgacgacca gaatgtgtgc
     4681 ggcgctcatg ctcgctcctt cctgggttca gcgcgccggt cgcgcgtaac caactgtcgg
     4741 gatccgtctg ttacgggcaa ggatacacgc acgcgaccac cgccttccgg ccgattgtcg
     4801 gcttccacgc gcccgccgtg ctcttcgaca atctttttga caatcgccag ccccaatccc
     4861 gtgcctttgg gcttgctggt gacatacggc tcgaacacgc gccccaatac ctcgcgggca
     4921 aagcctggcc cgttatcggt cacggtaatg gtggcgtatt cggcggagcc gacgctctca
     4981 aggcgcgttg cggcgacgac gtgaccaccg ggcgtgtttt ccaccgcttc ggcgccattg
     5041 acgatcaggt tgttcagaat ctggcgcact cggccgcggt cggcttcgac gctgtcgatg
     5101 cgcgggtcga gatcgaggtc gatcctcacg cccgattcct gcacgcggtg caggtccgcg
     5161 acttcggtca ccagcgcgtt gagcggaaat ttcgtgacct tcatatccgg agcacgcgcg
     5221 tattcgctga acgcattcac catggctttc atggcttcca cctgctgcac gatggtgtac
     5281 gtggcgcgat ccaggatctg tgcgtcttcc ccggacatct gatgaaggta acggcgccgc
     5341 atgcgttccg cggacagctg gatgggcgtg agcgggttct tgatttcatg ggccaggcgg
     5401 cgcgccacct caccccatgc cgcgtcgcgc tgcgcctgca gcaatgcgct gatgtcgtcg
     5461 aacacgatga cgtagccacc cagcgcggcg gcgtcgtctt cggcatgctc gtcaccgggc
     5521 aacggtgtgc aggcgcacac cagcacgcgc gcgccggccg cctcgggttt caggtcgagc
     5581 tgctcgcgcc attcttcttc gccgcgtgcg aggcgcatcg tgacttcgcc gacgaaccgc
     5641 tcgtagcgcg ggttttcgcc agacagcgcc gccagcggct ggccgactcc ctgttcgaga
     5701 ttcgcgccga gaatattgct ggccgcctgg ttggcgctgc gcagcaccag gtcgtgcccg
     5761 atggcgatga cgcccgtcga caggcgcgcc aggatgatcg acaggcgctc gcgttcgcgc
     5821 tccaccgcat tctggctgcg cgtgttctcc tcgcgcgcgc ggcgcaggcg cttggtcatg
     5881 tcgttgaacg aatgcaccag gaaccccatc tcgtcgcgcg acggcagcgg caggcgcgtg
     5941 ccgaaatcgc ctttgccgac cgcgcgtgtg ccggcgatca ggtcctgcac gggacgcacg
     6001 agcttctcgg ccgagtagat agcaccgtaa attgccgtga gcatcgccaa caggagcacc
     6061 agtgtcagag tcatgccaaa caggtttttc acgccctcac gctgctgggt gaggttgtag
     6121 tgttgtgaat acgagtgctg cacggcttcc gataagcgcg aaagctgcgg cggcaccggg
     6181 tactgcgcca cgacgaagcg accctgactc gaacctggcc cggatgcgat aggcgcggcc
     6241 gtattgatga ggtaatcgtc ctccgacagc ggttccaggc tcagatagag gttgccggcg
     6301 gcgatggcgc tcgcgacctc ggcggatacg cgcggcaggc tggtggaagt ggcgctggaa
     6361 ctgctcgcgg ccagcacctg gccgagcgtg ccgtacacca ccacttcgag cgcgccggtc
     6421 tcgacgcgct gcacgtcagt ggcttcggcc cagttggacg gcgggctgtc ggcgaggcga
     6481 tgcgcaaaca attcggtgct gcgcgcgtac tcgcgcatgc ggacgttgac cgccgatttg
     6541 gaaagctcga gcgcgtcggt gagtccctgc ttgatctcga ccttgaacca gctgtcgatg
     6601 ccctggttca ggaattcgag cgagaataga tagacgatga tgagcggcgc gatgaccagc
     6661 gaaccgaaga tggccaccgt gcgcgccgtc agccgcgagc ccgggacgcc ggcgcggaat
     6721 gcgcgcacca gctcgatgat cttgcgcgcc agcaggatgc tgaccgcgat gagcgcgatg
     6781 acgttggcga gcaggatcca tgcctgcagc cggctgaact gcatcgagtt ctgcaccgac
     6841 aacgccagca acagcagcag gccaccgctg accagcaccc agagggcgat cagccccagg
     6901 gtgcccaggc gtctcagtgc tgcagggacc atgagaacca ctcactctcg cggtgccagt
     6961 cgtcggtcca gaacatgagc acgcgcatgg tgtcggagag ccggccgcgg cgcacgccgg
     7021 cgcgcagggc cacgcgatac tggcggttgg tcgagagctg cggggacagg aggaatggcc
     7081 acgcatccac ggtgccgagt gcttcgagcg cttcttccag cgtcgcgtaa ctgtgttgat
     7141 cgaggttgcg gtcatcgacg tcgcgcgcca cgtagcgatc gctgaccgcg tgatagagaa
     7201 gttcccgccg cagggtgtat tcggccaccg tctcgtccac ccagaagcgg cgctcacgtg
     7261 acacgaccac atcgaggtcg aagttcaaca tcaggccgtc cttgagcgct gcgcgaatgt
     7321 cgtcgttgac cggataggta acgcgcgcaa agagctggaa cacgccctgc tccggcttga
     7381 cgtacgccga acggacctcg agcacaccat cgagcacgtc ggcgcgcacg accccgcctg
     7441 gcgacacgct caacagcgcg gcaagcagca gcagaccgag gttattcgcg cggaactgca
     7501 ttggttcctc ttagtgggtt gtcaggatag cttagtgaga caggcatagt agaagccatc
     7561 cgtgcccgcg attcctcccg gcagcaactg ccagccgacc gtccgatcga gcaggccagg
     7621 cggccgcgcg agaccttccg gccatggcgc cagcgccgcg cgcgggaacg cgtccaggaa
     7681 ttcggccacc accccttcgt tctccaccgg catcaccgag caggtgcagt agatcagacg
     7741 gccgccgggc ctcagtagtt cgaatgcggt ggcgagaatc tgccgctggg tggccgcgaa
     7801 ggattcgatg tcggccggcc ggcgaagcag cttgatatcg ggatggcggc ggatcacgcc
     7861 cgtggccgaa caaggcgcgt ccaccagcac ccgatcgaaa tgggcgagct gaagcgaagg
     7921 tggggcagtc cggaggtcgg ctttcaccag gatcgcgtcg cgccccgccc gctcgagatt
     7981 ttcccggaca cgcgccagcc gcagcgcgtc gtcatcgacg gcgatgagct cggcgagccc
     8041 ggcggtgcgt tgcgcgatgt gcagcgtctt gccgccgggc gctgcgcagg cgtcgagcac
     8101 tcgcatgccg ggctgcgcat cgagcaaggg tgcggcgagt tgcgcgccgc aatcctgtac
     8161 cgacaccgcg ccagtttcga aacccggcag cacctgcacg ggagcggggc gatccagcac
     8221 catcgcgtcg cgattccact cgactgcggt cgcatcgcgt ccgatggcac gcagcgagcg
     8281 cagaaaatct tcggcggagg taagcgccgg atcgagcctc agtaccatgg gtggatgctg
     8341 gttgttcgcg tcgaggattt cgggcgcgcg ctcactccaa gccgtgttca acgcctccac
     8401 cagccactgc ggatgggcgt gccgttgact cagtttttcg tcggccgtcg ccagcaactg
     8461 cgcgcgctcc gcgacgaacc ggcgcagcac ggcattgacg accccgctgg cgcgcccttc
     8521 ttcgagcacg cggctcgcgt ccacacccag gtggacctgc gcctcggcgg caccgcgcga
     8581 gtattcgacc tggtgcgccg tggtaaccag caacgccgcg agccggggag acaattcgtc
     8641 gaagggacgg ttgagcagag gcgtgagcgc gggcttgagc cgcaggtacc agcgcaacgt
     8701 tcccaaggtg atggcgcgta cggccgcgcg gtcttgtcgc tcgtgggcgg cttcgagtgc
     8761 cgcgtccgaa gaacgacctt cgaaaaccac atcgtggacg gcgagcgccg cggcggccag
     8821 ggtttcagcg cccggtgcgt attgggtgcg gcgcggtggc ggcggcggat tgcgacggac
     8881 tttcttcatg aaccgaacct caggcccgcg acgcgtacgg cgttggcgaa atcgcgtgcc
     8941 gatatcggcc gtttgccggc gcgctgcagc tcgcaaacgg cgagcacacc ctcgccgcag
     9001 gcgacccgca ggccgtcgtc ggcgaggcct aacaacgtgc ctggttcggc atggacgccg
     9061 cccggctcgg ccacgcgcgc gcgcagcacg cgcagggtct cattgccgaa acacgtttcg
     9121 gcgacgggcc agggattgaa cgcgcgcacc tggcgatcga gctggacggc gctcgccgac
     9181 cagtcgagtg cggcctccga cttttcgatc ttcggcgcat ggctcgcgcc cgtcgcgtgt
     9241 tgtgcgcgcg gccgaagcgt tccggccgca agtccatcga gcgcctcgat cagcgcgacg
     9301 gcgccgagtt ccgacagcgc gtcgtgcaga tcgcctgcgg tgtcgtgcag tccgatcgag
     9361 cggctgcgtt cgagcagcat ggggccggtg tcgagtcccg cgtcgagctg catgatggtg
     9421 acgccggttt ccgcatcgcc cgcgagaatt gcgcgctgga tcggcgcggc accgcgccag
     9481 cgtggcagca gcgaaccgtg gatgttcagg catcccaggc gaggcaggtc cagcgccgct
     9541 tgcggaagaa tgagcccgta ggcgacgacc acgagcacgt ccggcgccca ttgcgcgagg
     9601 tcggcgtgcc cctcgggagt cttgagcgtt gacggctgcg caatcggcag ccggtgttcg
     9661 gcggccagta gtttgaccgg cgatgcgcgc agatgctgtc cgcgtcccgc ggggcggtcc
     9721 ggctgggtca gcacgccaac gacctgatgc gtggacttga gcagagcgcg gagagcgggc
     9781 aacgcaaact gcggtgtgcc cgcgaaggca atacgaaagg aggtcatggg c
//
LOCUS       DASQJS010000289        11065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2684889, whole genome shotgun sequence.
ACCESSION   DASQJS010000289 DASQJS010000000
VERSION     DASQJS010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11065
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2684889"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..2544)
                     /gene="bamA"
                     /locus_tag="VEW08_11570"
     CDS             complement(154..2544)
                     /gene="bamA"
                     /locus_tag="VEW08_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HYJ41423.1"
                     /translation="MKWLIPLALLVSAPHAHADEAMEFIVGGIRVEGLQRISEGTVFN
                     TLPINIGDPIGPRRIREALRALNDTGFFRDVELRRDEAVLVVVVQERPSIRTFQVTGN
                     KDIKSEDLMKSLRNVGLASGKILNRSTLEDTRQYLIEQYFARGRYDVRVDAKVEEQPG
                     NLVDVHVEIVEGKHVRIRGINVVGNEHFTDEELLSGMALKSSNWLSFYRGDDKYSRHA
                     LEGDLEKLRSYYMDRGYADFEITSTQVTISPEKDDLFITLNVFEGAIWKAGAVKLAGR
                     FVVPEEVLRQYVLVRPGDQYSQRLIAATEEALRNRLSEAGFGFAEVATVPNANPATGE
                     IGLTFMIEPNARTYVRRINFEGVEKTRDEVLRRELRQLEGAVLSNAAIARSEERLQRL
                     PYIEKVESETKRVEGTPDLVDIDVTVEEGPSSTLGGGVGYSERQSFTLSGNFVDSNLF
                     GSGDRLAAEFNGGRYGQLYSVAHTDPYFTVDGISRSLNASYVERERITSSFSQFTTQT
                     YSVGASAGYPIAEEQYLNLGFVYSHENLATASSSSTQLRDWVRNNGDYYFRRVGTDRV
                     LGTILDTFEITGGWLYDSRDRTLFPTRGGSHRLSLAFAPPSNEVSYASANFRSQQFFR
                     IPGFDLLDRFPLSVTTNLGWSTAFGETTGVPPHRHVFTGGSDSVRGFRDGSLGPRDSL
                     GNPYGGDAGVSAQFEAIVPLPAKFASAARVSLFYDIGQSFYLGDTEFRNKRGDRTDYR
                     FDLGELRSSAGISVQWLSPMGLFRFSWAVPLRYQGETRLEFGDELDPFQFSVGTAF"
     gene            2687..3406
                     /locus_tag="VEW08_11575"
     CDS             2687..3406
                     /locus_tag="VEW08_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ41424.1"
                     /translation="MATQKHILIVDEDREARALLASALERSGYRVTTASSGGGMQRAL
                     ERARIDLVVLDVCFSEEDGMRVCRSLRAASDVPFIILAGRADDVDRILGLEMGADDYL
                     AKPVNSRELLARMRNILRRVNGAGTPAMNVRARLFRFGGWQLDSVARELCDPQGRRAT
                     LRNSEYRVLASLLAHGNRVVSRGKLIELARGRDAGPFDRSIDVRISRLRQVFGDDARG
                     PRIIKTVHGEGYVIGVPVERA"
     gene            3492..4097
                     /locus_tag="VEW08_11580"
     CDS             3492..4097
                     /locus_tag="VEW08_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYJ41425.1"
                     /translation="MKIADEDTDCMARLAAGDAHAVHELYQRYGRALLRFSSAMCRSR
                     QAAEDMVHDTFVALMRERISFDPAQGSVYGYLCGVLRHRVSRHFRQQRRWVALDADDD
                     TSPVHAVDSSGPADEISRSEITAAFRRAMLELPLQHREVIALCDLEELPYVTVAGILD
                     CPVGTVRSRLHRARALLTIRLASLELIDLQVEPSMVQRGLT"
     gene            4094..4642
                     /locus_tag="VEW08_11585"
     CDS             4094..4642
                     /locus_tag="VEW08_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41426.1"
                     /translation="MNEELTGNRHSHTALLLRQYARDLAQIAPSSDLDARIDALVAAG
                     RQNSPASHGPVRRPRRLLPFAAAAGIAALAIIFGILIGMRLEQSRELAAANSGQTHEP
                     AWPTADLTMWPSDSVSFKIPAEYSAQGTLVAVDPNTRNTSTRYWIDVVVSNDGTFRIQ
                     KIVPADPKQKQGAPDGIAPHIQ"
     gene            4617..5414
                     /locus_tag="VEW08_11590"
     CDS             4617..5414
                     /locus_tag="VEW08_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41427.1"
                     /translation="MASRRIFSRALALLGAFGLGVLSLHAQAQDQSREAAIPDCPPAP
                     ASGSTAVAHSTPDNVRLVTGAPYSALGTSQTVTTLPDGNRVVRQNTIRLWRDSTGRTR
                     SEFSLSSIGGPTPLEVNATVTVIDDPSTRERHMLQPDGRVVTVPIAPCRVLASNEPDL
                     TVGPPRPAGLPLKVSQPVKLGERQLDGETVAGSRVEATIPAGAVGNDHPIKMSAEQWY
                     GKDLQVVVEATYRDPRTGETRYKLREIRRNEPDASLFEVPAGGIKRR"
     gene            complement(5401..6903)
                     /locus_tag="VEW08_11595"
     CDS             complement(5401..6903)
                     /locus_tag="VEW08_11595"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HYJ41428.1"
                     /translation="MSAAIRDPGMREVVVVGRDAPLWLSACVMQYALGPAGVTVTVVE
                     LPPLAQPADVCITLPALEPLHTRLRIDEARLIAATRGAFSLGRHYVDSGGKAPPFFHA
                     HGSNGSRIDEKEFLPHWLLARRQGLNSPFEEFSLTAAAARAGRMLLPDAAIDRFGFTD
                     YAYHLPAIPYAAWLRQLALRRGVRNHWSSTLEVRLDAEGAIAELLLEGDRRVKGDFFL
                     DVTGGEALLASAMGVGRESWRESFVADRVMSAHSAPVSPVPIYSEVRAHARGWVALAA
                     SQVCIHVSQAYCSDIAPGPADLAAAAGIPLQGMVVRERHPGRRLRAWEKNCVAVGEAA
                     CVFDPLHFLDLHAVQAGLVHLLPLFPVQSDFSVERDEYNRNVHSAFERMRDFQSAHYR
                     LNRYGDSPFWARARGAALSAELTHKLDAFRARGETVDYEDEAFTIDDWQALFIGHGVV
                     PESYDPAVDRTSPDLLKSELARILGFIRQKVDEQRSHSDYLRDLSRVSGA"
     gene            complement(6900..8438)
                     /locus_tag="VEW08_11600"
     CDS             complement(6900..8438)
                     /locus_tag="VEW08_11600"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HYJ41429.1"
                     /translation="MQPSPLRIVIVGGGSAGWMCAAACAQVLHPARYSLTLIESDDIG
                     TVGVGEATLPALQDFNKLLQIDEAQFMRETQGTFKLGIEFRGWKRPGESYVHPFGAFG
                     QLWGGVEFQHFWLRARQLGLNPPPLDEFSCAVRACARNAFEFPDTHSVRSTFYGYAYH
                     FDAGLYAEFLRDWATARGVARIEGQVVDVAQESTQGDIKSLTLKSGQQISGDFFIDCS
                     GFRSLLLADKLGVPWEDWSPWLPCDRAWAVPCERSTDFTPYTRSTAQKGGWVWRIPLQ
                     HRTGNGHVFSTQFVGEDEARSTLLAGLDGKALGDPRLLKFRAGRRVQAWKRNCIAIGL
                     ASGFLEPLESTSIFLVQKAIQDMLRLLPSPEARGVDERVVREFNRRSDELYERIRDFL
                     ILHYTANDRIGEPLWDHVRHYELPESLRHKLRLFDVRGHVPYFKDGFFSRDSWLAVLF
                     GQGLMPKAYDRLADFMPREQLDARLHDLHARVAAEVAAMPTHEEVVARYCSEPRFTPR
                     VMTS"
     gene            complement(8555..>11065)
                     /locus_tag="VEW08_11605"
     CDS             complement(8555..>11065)
                     /locus_tag="VEW08_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ41430.1"
                     /translation="RTGAALTLQYENDAKNLLLTLTGVHSKYENPWIERSANISWPTG
                     AGFGTPVWAPFGAPAWRPITGNFTFADNGMLQSGVIGQPADQPGFYEGTNAANINHGS
                     AVPGLPFVNGSEACGGTPCITGSNVSNESRIFDHEEQTQDFSFNVKWDITENLHTGFD
                     VQYIKAKTNNYDILVANNSLAQVDYTTDGDGTPRIALSPGPNTNYADGFLSNPHNYWM
                     QFIQDHWEDNDADEVAARADVEYDLGSGGWLSSLKAGVRFADREQKVRYSSYNWAPIA
                     PAWGCNGPGFNIDNTSAAPYPAACGNANPFNGYPAGIWETANLSDHFDGSVFPNDPLV
                     FLTNATLRDYDLHTEGLADRNINAPQGWNPLCDRTANVEGEGCFTAPEILDVREKSKA
                     AYLMLRFGGPEANLGNVNVVGNVGVRFVRTEVESHGQVSFPTSQWYTNALASGQGACD
                     PANNGANQATDIQCWLTPSLQAFSSGTGTDNTLDKTYTNVLPSFNVRFGFTDTQFMRF
                     GYSKGMSRADFGLLRNSVAINAPPIDTSNSSPYLIRNASGQVTGYNFVFSAEAGYAGL
                     EPTEADNFDLSYEWYMDSSSSFTLGLFYKRLENAVAYGRAVRDIENGGSTQSVTIRGP
                     SNDPSSAGTLKGFEIAYQTFFDSLPGAWAGLGVQLNYTHTDQDDINNSNLAVQAGYLP
                     GSTTAFGGGNNGSTGGQGNAGNPLSFTSNVIDSHRLAGISDDSYNIVGLYEFGKFGAR
                     LAYSWRSEFLTANLDCCIGLPVWQKDNGYMDGSFRYAINDNVEMSFDVSNILNTTAVT
                     QQQVFGDSTLTPGAKAVKLDSGWVNNDRRFQLGVRFKY"
BASE COUNT         1993 a         3582 c         3499 g         1991 t
ORIGIN      
        1 ataacccgcg ctcaggttga gcaggcggat cttcgggtct tctccaccat cctcatcacc
       61 gccgccgcag gcgttgagcg cgagcgtcgc cacgagaccc aggacccaag ttgctccgtt
      121 acgaattctc attccattct ctccatctat gcgtcagaac gcggtgccga ctgaaaactg
      181 aaagggatcg agctcgtcgc cgaactcgag tcgtgtctca ccctggtaac gcagcggtac
      241 tgcccaggaa aatcggaaca agcccatggg cgacagccac tgcacggaga tgcctgccga
      301 actgcgcagc tcacccaggt cgaagcggta gtcggtgcgg tcaccccgct tgttgcggaa
      361 ttcggtgtcg cccaggtaga acgactgccc gatgtcgtag aacaggctca cgcgcgccgc
      421 gcttgcgaac ttcgccggca gcggcacgat ggcttcgaac tgcgccgaca cacccgcatc
      481 accgccgtaa ggattgccca gcgaatcgcg cggcccgagc gagccgtcgc ggaatccgcg
      541 caccgaatcg ctgccgcccg tgaacacatg acgatgcggc ggcacccccg tggtctcacc
      601 aaatgccgtg gaccagccga gattggtggt gaccgacaac gggaatcgat cgagcaggtc
      661 gaatccggga atcctgaaga actgctgcga acggaagttc gcggaggcgt agctcacctc
      721 attgcttggc ggcgcaaatg cgaggctcag gcggtgcgag ccgcctcgcg tcggaaacag
      781 cgtgcggtcg cggctgtcgt agagccatcc gccggtgatc tcgaaggtat cgagaatcgt
      841 gcccagaacc cggtccgtcc ccacacggcg aaaatagtag tcaccgttgt tgcgcaccca
      901 atcgcgcagc tgcgtcgagc tgctggaggc cgtcgccagg ttttcgtggc tgtagacaaa
      961 tccgaggttg aggtactgct cttcggcgat cggatagccg gcgctggcgc cgacgctgta
     1021 ggtctgcgtc gtgaactgcg aaaacgacga ggtaatccgt tcccgttcca cgtatgaagc
     1081 gttgagcgag cgggagatgc cgtcgaccgt gaaatacgga tcggtgtgcg caacgctgta
     1141 tagttggccg tacctgccgc cgttgaactc ggcggcgagc cggtccccgg aaccgaacag
     1201 gttgctgtcg acgaagttgc cgctgagcgt gaacgactgc cgctccgagt agccgacgcc
     1261 cccgccgagt gtcgacgacg gcccctcttc gaccgtgacg tcgatgtcga cgaggtcggg
     1321 cgttccctcc acccgcttcg tctccgactc caccttctct atataaggga ggcgctgcag
     1381 gcgctcctcc gaacgcgcga tcgccgcatt cgacaacact gcaccctcga gctgacgcag
     1441 ctcacggcgc agcacctcat cgcgggtttt ctccacacct tcgaagttga tgcgccggac
     1501 gtaagtgcgc gcattcggct caatcatgaa agtcagcccg atctcgccgg tagccggatt
     1561 cgcgttcggc accgtggcga cctccgcgaa tccgaaaccg gcttcgctca ggcgattgcg
     1621 cagcgcttcc tccgtggcgg cgatgagacg ctgcgaatac tggtctccag gccggacgag
     1681 aacgtattgc cgcagcacct cttcgggcac gacgaaacgg cccgccagct tgacggcgcc
     1741 cgccttccag atggcgcctt cgaacacatt cagcgtgatg aacagatcgt ccttctccgg
     1801 cgagatcgtc acctgcgtcg aggtgatctc gaagtccgca tagccgcggt ccatgtaata
     1861 agagcgcagc ttttccagat cgccctcgag cgcatggcgc gagtacttgt cgtccccgcg
     1921 gtagaacgac agccagttgg aagacttgag tgccatgccg gagagcagtt cttcgtcggt
     1981 gaagtgctcg ttacccacca cgttgatccc gcggatgcgc acgtgcttgc cctcgacgat
     2041 ctccacgtgc acgtcgacca ggttcccggg ctgctcctcg accttcgcgt cgacgcgcac
     2101 atcgtagcgg ccgcgcgcga agtactgctc gatgaggtac tggcgcgtgt cttcgagcgt
     2161 cgagcggttg aggattttgc cgctggccag gcccacgttg cgcagtgact tcatcaggtc
     2221 ttcgctcttg atgtccttgt tgcccgtcac ctggaacgtg cggatcgaag gccgctcctg
     2281 caccaccacc accagcaccg cttcatcgcg gcgcaattcc acgtcgcgaa agaagccggt
     2341 gtcgttgagc gcacgcagcg cctcgcggat gcgtcgtgga ccaatcgggt cgccaatgtt
     2401 gatgggcagc gtgttgaaca cggtgccttc gctgatgcgc tgcaatcctt cgacgcggat
     2461 gccaccgacg atgaactcca tggcttcatc ggcatgcgca tgcggcgcac taactagcaa
     2521 ggcgagcggg atcagccatt tcattccgca atacgaccgg gaacgcgcgt ttcggttcga
     2581 tatgaattgt tacgtagtgt tacgaaacgt aaccgcttgc atcgatggaa ccattcgcgc
     2641 cgctcgtggc aatagcgctg gatggtgcct cgctgacgca atggacatgg ccacacaaaa
     2701 acacattctc atcgtcgacg aagaccgcga ggcgcgagca ctgctcgcgt cggcgctgga
     2761 gcggtccggc tatcgcgtga ccacggccag cagcggcggt ggcatgcagc gcgcactcga
     2821 gcgtgcccgc atcgacctcg tggtgctcga cgtctgtttc agcgaggagg acggcatgcg
     2881 tgtctgccga agcctgcgtg cggcgagcga tgtccctttc atcatcctcg cgggccgcgc
     2941 cgacgatgtg gaccgcattc tcggcctcga aatgggcgcg gacgactatc tcgcaaagcc
     3001 cgtgaattcc cgcgaattgc tggcgcgcat gcgcaatatc ctgcggcgcg tgaatggcgc
     3061 gggcacgccg gccatgaacg tgcgggcacg gcttttccgc ttcggcggat ggcagctcga
     3121 cagcgtggcg cgcgagctgt gcgatccaca gggacggcgg gccaccttgc gcaactccga
     3181 atatcgcgtg ctcgcatcgc tgctggcgca cggcaatcgc gtggtgtcgc gcggcaaact
     3241 catcgagctc gcgcgcggcc gcgatgccgg gccattcgat cgcagcatcg acgtgcgcat
     3301 cagccggttg cgccaggttt tcggcgacga tgcgcgcggg ccacgcatca tcaagacagt
     3361 gcacggtgaa ggttatgtca tcggtgtccc cgttgaacgc gcttgaacaa cgctggaact
     3421 tttcgcgcgc gcatcgcccc aatgctggcg tcgcacgatt gcgttgcgca ggggaagcaa
     3481 cggctcatca catgaaaata gcggacgaag atactgactg catggcgcgg ctcgccgcgg
     3541 gcgacgcgca tgccgtgcac gagctctatc aacgctacgg tcgcgcgttg ctgcggtttt
     3601 cctcggcgat gtgtcgctcg cgccaggcgg ccgaagacat ggtgcacgac actttcgtgg
     3661 cgctcatgcg agagcgcatc tcgttcgatc cggcgcaggg cagcgtgtac ggctatcttt
     3721 gcggcgtctt gcgtcatcgc gtgagccgcc atttccgcca gcagcggcgc tgggtcgcgc
     3781 tcgatgcgga tgacgacacc tctcccgtgc atgcggtgga ttccagcggc cccgcggatg
     3841 aaatctcgcg ctcggagatc acggccgctt ttcgccgcgc catgctcgaa ctgccactgc
     3901 aacaccggga agtcatcgcg ctgtgcgacc tcgaagaact gccatatgtg acggtagccg
     3961 gaatactcga ttgccctgtg ggcacggttc gctcccgcct gcatcgcgca cgtgcgctgc
     4021 tcaccatccg cctcgcctcg ctcgagctga tcgacctgca ggtggagccg tcgatggtgc
     4081 aacgaggcct cacatgaacg aagaactcac cggcaaccgg cattcgcaca ccgcactgct
     4141 gctcaggcaa tacgcacgcg acctcgcgca aattgcgccc agttccgatt tagatgcgcg
     4201 aattgacgct ttggtcgcag ccggccggca gaattcgccg gcaagtcatg gccccgtccg
     4261 gcgcccgcgc cgcctgctgc cattcgccgc ggcagccggc atcgcggcgc tggcgatcat
     4321 cttcggaatc ctcatcggca tgcggctcga acagtcccgt gaattggcgg ctgcaaactc
     4381 cggacagact cacgagccag cctggcctac ggcggacctc accatgtggc cctcggattc
     4441 agtgtccttc aagattccgg cggaatactc ggcccagggc acgctggtgg cggtcgatcc
     4501 gaacacccgg aacaccagca cgcgctactg gatagacgtt gtcgtctcga acgacggcac
     4561 gtttcgcatc cagaagatcg tcccggcaga tccgaagcag aagcagggag cgcccgatgg
     4621 catcgcgccg catattcagt agggcgctcg cccttcttgg cgcattcggc ctgggcgtgc
     4681 tcagcctgca tgcgcaggcc caggaccagt cacgggaggc cgcgatcccc gactgcccgc
     4741 ccgctcctgc gtccggcagc accgcggttg cccattccac gcctgacaat gtccggctcg
     4801 tcaccggcgc gccatattca gctctgggca ccagccagac cgtcaccaca cttccggatg
     4861 gcaatcgcgt cgtgcgccag aacacgattc gcctgtggcg cgacagcacg gggcgtacgc
     4921 gctcggagtt ttcgctctcg tccatcggcg gacccacacc actcgaggtc aacgccaccg
     4981 tcacggtcat agacgacccg tcgacgcgcg agcggcacat gctgcagccc gacggccggg
     5041 tcgtcaccgt gcccatcgcg ccgtgtcgcg tgctggcctc gaacgaaccc gatctcaccg
     5101 taggtcctcc gcgacccgcg ggattgccgc tcaaggtctc gcagccggtg aagctcggcg
     5161 agcgccagct cgacggcgaa accgtggccg gcagtcgcgt cgaagccacc atccccgcag
     5221 gggccgtcgg caacgatcac cccataaaga tgtctgccga acagtggtat ggcaaagatc
     5281 tgcaggtggt ggtcgaggct acctaccggg atccgcgcac gggcgaaacc cgttacaaac
     5341 ttcgcgaaat ccgccgcaac gagccggacg cttcgctgtt cgaggtgccg gcaggcggca
     5401 tcaagcgccg ctgactcggc tgaggtcgcg cagatagtcc gagtgcgatc gctgctcatc
     5461 gaccttctgc cggatgaatc ccagaatgcg ggcaagctcg cttttcagca gatccggcga
     5521 cgtacgatcc accgccggat cgtacgactc cggcactacg ccgtggccga tgaacagcgc
     5581 ctgccagtcg tcgatggtga aagcctcgtc ttcgtagtcc acggtttccc cgcgagcgcg
     5641 aaaggcgtcc agcttgtgcg tgagttcggc gctcaaggct gcgccccgcg cccgcgccca
     5701 gaacggcgaa tcgccatatc gattcaggcg gtagtgtgcg ctctggaagt cgcgcatgcg
     5761 ttcgaaggcc gagtgcacgt tcctgttgta ttcgtcacgc tcgacgctga agtcggattg
     5821 caccgggaac aggggcagca gatgcaccag gcccgcctgc accgcatgca gatcgagaaa
     5881 atgcagcgga tcgaatacgc aggcggcctc ccccaccgcc acgcagttct tttcccaggc
     5941 gcgcagacgc cgccccggat gccgttcgcg aaccaccatg ccctgcagcg ggatgccggc
     6001 ggcggccgcg agatccgcag gaccgggcgc gatatcgctg caatacgcct ggctcacgtg
     6061 gatgcacacc tggcttgcgg ccagcgccac ccagcccctg gcatgggcac ggacctcgga
     6121 gtagataggg acgggtgaca cgggcgcgga gtgcgcgctc atcacgcggt cggccacgaa
     6181 tgactcgcgc cagctctcgc gccccacgcc cattgcgctg gccagcaacg cctcgccgcc
     6241 ggtcacgtcg agaaagaagt cgcccttgac gcggcgatcg ccctcgagca gcagctccgc
     6301 aatcgcgccc tcggcgtcga ggcgcacttc gagcgtgctg ctccagtggt tccgcacgcc
     6361 gcgccgcaag gcgagctggc gcaaccatgc cgcataggga atggccggca ggtggtacgc
     6421 atagtcggtg aatccgaagc gatcgatcgc ggcgtccggc agcaacatgc ggcccgctcg
     6481 tgcggccgcc gcggtgagcg agaattcttc gaatggcgaa ttcaggccct ggcgacgcgc
     6541 gagcaaccag tgcggcagaa attctttctc gtcgatgcgt gagccattcg agccatgcgc
     6601 gtgaaagaac ggcggcgcct tccccccgga atcgacgtag tgccggccga gtgagaacgc
     6661 cccgcgcgtg gcggcgatga gacgcgcctc gtcgattcgc aggcgagtgt gcagcggctc
     6721 gagcgccggc aaagtgatgc agacatcggc gggctgcgcg agcggcggca gttccaccac
     6781 ggtcaccgtg acaccggcgg ggccgagtgc gtactgcatc acgcaggccg agagccacag
     6841 cggggcgtcg cgcccgacta cgacgacttc acgcatgccg gggtcgcgga tcgcggcgct
     6901 cacgacgtca tcacccgtgg agtgaagcgt ggctcgctgc aataacgcgc gacgacctct
     6961 tcgtgcgtgg gcatggcggc cacttccgcc gcgacacgcg catgcaggtc gtgcaggcgg
     7021 gcgtcgagtt gttcgcgcgg catgaaatcg gcgagccggt cgtaggcttt cggcatgagg
     7081 ccctggccaa agagcacggc cagccagctg tcacgcgaga aaaagccatc cttgaaataa
     7141 gggacgtgac cacgcacatc gaacagcctg agcttgtggc gcaggctctc cggcaactcg
     7201 tagtgacgca cgtggtccca gagcggctca ccgatgcggt cattggccgt gtagtgcagg
     7261 atcaggaagt cgcggatgcg ctcgtacagc tcatcggagc gccggttgaa ttcgcgcacc
     7321 accctttcat ccactccgcg cgcttcgggc gatggcagca ggcgaagcat gtcctgtatc
     7381 gccttctgaa ccagaaaaat actcgtcgac tcgagcggct cgagaaagcc gctggcaaga
     7441 ccgatggcga tgcagttgcg tttccacgcc tgcacacggc gaccggcgcg aaacttcagc
     7501 aggcgcgggt cgcccagcgc cttgccgtcc agccccgcca acagcgtgga gcgtgcttcg
     7561 tcttcgccga cgaactgggt cgaaaacacg tgaccattgc cggtgcggtg ctgtagcgga
     7621 atacgccaga cccagccacc cttctgcgcc gtggaacgcg tgtacggcgt gaagtcggtg
     7681 gatcgttcgc agggcacggc ccaggcgcgg tcgcagggca accatgggct ccagtcttcc
     7741 cagggcacgc ctaacttgtc ggccagcagc agcgagcgga acccgctgca gtcgatgaag
     7801 aagtcgccgc tgatctgttg tccggacttc agcgtcagcg acttgatatc accctgtgtg
     7861 ctctcctggg cgacgtcgac cacctgccct tcgatgcgcg cgaccccgcg cgcggtcgcc
     7921 cagtcgcgca ggaattcggc gtagaggccg gcatcgaaat gataggcata gccataaaag
     7981 gtggagcgca cggagtgcgt atcgggaaat tcgaaggcat tgcgtgcgca cgcgcgcacc
     8041 gcgcaggaaa actcgtcgag cggtggtgga ttgagtccca gttggcgggc tcgcaaccag
     8101 aagtgctgaa actccacgcc accccagagc tggccgaacg cgccgaaagg atgaacgtaa
     8161 ctttctcctg ggcgcttcca gccgcgaaac tcgatgccga gcttgaacgt gccctgcgtc
     8221 tcgcgcatga actgcgcctc gtcgatctgc agcagcttgt tgaagtcctg cagcgccggc
     8281 aacgtggctt cgcccacgcc cacggtgccg atgtcatccg attcgatcag cgtcagcgaa
     8341 tatcgcgccg gatgcagcac ctgcgcgcag gcggcagcgc acatccagcc ggcggacccg
     8401 ccgccaacga tgacaatgcg caaaggcgat ggctgcacca cctctctcct cttatcaatt
     8461 gacggtcgcc gccccttcag ggcggcgatt gatttcgcgc ggtagcgcta gaaaagagcg
     8521 ccgccggggg gaccgaacgg cgctcgtgtc ggcgtcagta cttgaatcgt acgccgagtt
     8581 ggaaccgtcg atcgttattc acccatcccg aatcgagctt caccgccttc gcgcccgggg
     8641 tcaacgtgga atcgccgaac acctgctgct gggtcacagc agtggtgttg aggatgttcg
     8701 agacgtcgaa cgacatctcc acgttgtcgt tgatcgcgta acggaaggag ccgtccatgt
     8761 agccattgtc cttctgccac accggaaggc cgatgcagca atccaggttg gcagtcagga
     8821 attccgaacg ccagctgtag gcgagccgcg cgccgaactt tccgaactcg tacaggccaa
     8881 cgatgttgta cgagtcgtcg gagatgccag cgagacggtg cgagtcgatg acgttgctgg
     8941 tgaacgacag cggattgccg gcgttgccct ggccacccgt ggagccgttg ttgccaccgc
     9001 caaacgccgt cgtgctgccc ggtagatagc cggcctgcac cgcgaggttg gagttgttga
     9061 tgtcgtcctg gtccgtgtgt gtgtagttga gctgcacacc caggcccgcc caggctcccg
     9121 gaagggagtc aaagaaggtc tggtacgcaa tttcgaagcc cttgagcgtg cccgcgctgc
     9181 tcggatcatt gctcgggcca cgaatggtca ccgactgcgt gctgccacca ttctcgatgt
     9241 cgcgcaccgc ccggccatac gccacggcat tttcgagccg cttgtagaac aggccgagcg
     9301 tgaacgagct gctcgagtcc atgtaccact cgtacgagag gtcgaagttg tcggcttccg
     9361 tgggttcgag gcccgcgtaa cccgcttcgg ccgagaacac gaagttgtag ccggtgacct
     9421 gccccgacgc gttgcggatc agatacggcg agctgttgct ggtgtcgatg ggcggcgcat
     9481 tgatcgcgac cgaatttcgc aacaggccga aatccgcacg cgacatgccc ttcgagtagc
     9541 cgaaccgcat gaactgcgtg tcggtgaacc cgaagcgcac gttgaagctc ggcaacacgt
     9601 tcgtgtaggt cttgtcgagc gtgttgtccg tgcccgtgcc gctgctgaac gcctgcagcg
     9661 atggtgtcag ccagcattgg atgtcggtcg cctggttcgc gccgttgttg gccgggtcac
     9721 aggcgccctg tcctgacgcc agcgcattgg tgtaccactg cgacgtcggg aagctgacct
     9781 ggccgtggct ttccacctcg gtgcggacga agcgcacgcc gacgttgccc accacattca
     9841 cgttgccgag gttggcctcg ggtccgccaa accgcagcat gagataggcg gccttgctct
     9901 tctcgcgcac atcgaggatt tcgggcgcgg tgaagcatcc ctcgccttcc acgttcgccg
     9961 tgcgatcgca aagcgggttc cagccctgcg gcgcgttgat gttgcgatca gccaaccctt
    10021 cggtgtgcag gtcgtagtca cggagcgtgg cgttggtcag gaaaaccagc gggtcgttgg
    10081 ggaacacgct gccgtcgaag tgatcggaaa gattcgcggt ttcccagatg ccggcgggat
    10141 aaccattgaa gggattggca ttgccgcagg ccgccggata cggggctgcc gacgtgttgt
    10201 cgatgttgaa gcccgggcca ttgcagcccc aggccggcgc gatcggcgcc cagttgtagc
    10261 tcgagtagcg caccttctgc tcgcgatcgg cgaatcgcac gcccgctttg agcgagctca
    10321 gccagccgcc gctgcccagg tcgtattcca cgtcggcacg cgccgcgact tcatcggcgt
    10381 cgttgtcttc ccagtggtcc tggatgaact gcatccagta gttgtgcggg ttagaaagaa
    10441 agccgtcggc atagttcgtg ttcggcccgg gcgacagtgc gatcctcggc gtgccatcgc
    10501 catccgtggt gtagtccacc tgcgcgaggc tgttgttggc cacgaggatg tcgtagttgt
    10561 tggtcttcgc cttgatgtac tgcacgtcga agccggtatg caggttctcg gtgatgtccc
    10621 acttgacgtt gaacgagaag tcctgcgtct gctcttcgtg gtcgaagatg cgcgattcgt
    10681 tgctcacgtt cgagccggtg atgcagggag tgccgccgca ggcctcgctg ccgttcacga
    10741 acggcaatcc cggcaccgct gaaccgtggt tgatgttcgc ggcgttcgtg ccttcgtaga
    10801 agccgggctg gtcggccggc tggccgatga cacccgattg caacatgccg ttgtcggcaa
    10861 aagtgaagtt gccggtgatg ggccgccagg ccggtgcgcc gaacggcgcc cacacgggag
    10921 tgccgaatcc agcgccggtc ggccagctga tgttggcgct gcgctcgatc cacggattct
    10981 cgtatttcga gtgaacgccg gtcagcgtca gcaggaggtt cttcgcgtcg ttctcgtact
    11041 gcagcgtgag cgccgcgccg gtgcg
//
LOCUS       DASQJS010000290         5371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4569993, whole genome shotgun sequence.
ACCESSION   DASQJS010000290 DASQJS010000000
VERSION     DASQJS010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4569993"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1468
                     /locus_tag="VEW08_11610"
     CDS             89..1468
                     /locus_tag="VEW08_11610"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HYJ41431.1"
                     /translation="MKPLTHVLCIALAFASLPALADPFDSWLASNAIPLRTLSVTDED
                     FGDLEPLMAAIGDARVVMLGEPSHGAGSAFAAKARLVRFFHQRMGFDVLAWESGLFDV
                     RLAQAALADASLDPSKAAQAGILPVWSRAAEVRPVFEYARATLATSRPLELVGFDINA
                     SAPKTNEKLATEMRAFFTAVREPALRDGGEKLADELIAAFKRVQARDEPKPGQGDLDN
                     LHRAAEQLDALLVQRRIDFMQVHAMRDVELMQHLVANLESAGTNAWHRRCCEAPNGDA
                     AIQLQTAEWNRRDLSLATNLRWLLEKRYAGRKAVVWAHNAHLMRAYFAADWNGVHAKP
                     QRNGMTPMGAIVAQWLKDDVYTIALTTYEGEEAWANGQKRGTISPAPAGSLEARLHAL
                     GEPHLFIDLRQARGVRSHPLRRPTTLRISGYGQPTGKYGNESVADITQAFDGVLFIDR
                     MAPATRLPD"
     gene            1579..3411
                     /locus_tag="VEW08_11615"
     CDS             1579..3411
                     /locus_tag="VEW08_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41432.1"
                     /translation="MSVPVGRRGFVAAAGALTLAPRIFAATDRTELLIRTPMAAPEWA
                     LLQRELLNAHTEACETFHARYFDERNHLLCFPRWGTNDGPDDAIEHVNDWPLVHALGG
                     SNRVLDLYRAVYEGHIEQYSALRTKSVPMGRQGMYVREFPPQLDWQHISEGLSVFNLM
                     GLSTPTDTRLIERARRFAGFYDGSDPAAPNYDRKHRVIRSMFNGSLGPLLRPTTALDW
                     AGDPFDASRFKLEHGERNFEEFLAHFQEYTDTVCDSPLNLQSTTLALNAWLLTGEKRW
                     RDWLLSYVDAWVERAGANDDILPSKVGLDGRIGKWYEGVYGWAFSPVVPQTGKREDRN
                     RVPRSIVAFMNAALLTGDDRYMAVWRRQNAAINSHAKEIDGQLQTPRMCGSAGWYSYA
                     PGPYRLNGFEIWYLSMSSEDRALAGSHPWVDFLEGRNPGYPVTALRASLERLRARVAL
                     VQADKSTPETRLADNALEYNPASVASLLQLTSGALHIARPPWSSTSPNQGGAPLHARL
                     RYFDPVRRRAGLPEDVGALVDKLEAHRTEVTLVNLHPNRERVVTVQGGAYGEHVILSA
                     HDGSRAHAVDGRSFDVRLAPGCGARLSLMMRRYSANPTLAFPWA"
     gene            complement(3421..4335)
                     /gene="kdgD"
                     /locus_tag="VEW08_11620"
     CDS             complement(3421..4335)
                     /gene="kdgD"
                     /locus_tag="VEW08_11620"
                     /EC_number="4.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF002958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047448 - 5-dehydro-4-deoxyglucarate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HYJ41433.1"
                     /translation="MKPADMAARIARGLLSFPVTHFDAEDRFDADAYRKHVSWLLENE
                     AAGLFAAGGTGEFFSLKPAEVGEVVSAAVAETKGRVPVIAGCGYGTAMAIELARDAEK
                     RGADGLLLLPPYLVNSEQAGLAAHIEKVCKAVSIGVIVYNRDNAIVTEETLAGLCERN
                     ANLVGFKDGVGDLELLARVYSRLGKRVTYIGGMPTAETYALPYHDIGFDTYSSAIFNF
                     MPMWAKRFFGAVRGGDRATIQHHLRDFVMPYIAIRNRAKGYAVSIVKAGMRIVGRPAG
                     HVRSPLTDLKPAEVEELRKLIERAQKLN"
     gene            4412..4783
                     /locus_tag="VEW08_11625"
                     /pseudo
     CDS             4412..4783
                     /locus_tag="VEW08_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029308938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
     gene            4863..5087
                     /locus_tag="VEW08_11630"
     CDS             4863..5087
                     /locus_tag="VEW08_11630"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HYJ41434.1"
                     /translation="METLNEMSIAESRRVFELCDLNGDGFLDQDEFHVLLEALDGDVS
                     RAECLLDFEIADTAGDGYIEFREFVAWWTN"
     gene            complement(5084..>5371)
                     /locus_tag="VEW08_11635"
     CDS             complement(5084..>5371)
                     /locus_tag="VEW08_11635"
                     /inference="COORDINATES: protein motif:HMM:NF025391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4241 domain-containing protein"
                     /protein_id="HYJ41435.1"
                     /translation="FLDFAAAALMGQRYRQEGPYFAFGDLLEEELEMTYRHTRNWAVV
                     RPIEEREENIICFSSGYGDGAYPSFFGFDADGKVCQLVTDFLIFGEAAGSP"
BASE COUNT         1002 a         1738 c         1641 g          990 t
ORIGIN      
        1 aatcgggtcc ccatcagatg taacacgatc tccagggcaa cgggaacgct tgcaaacctt
       61 ttgccaccac gcaacgtcga atgaattcat gaagccgctc acccacgtac tctgcattgc
      121 cctcgctttt gcgagtcttc cggccctcgc cgatccgttc gactcctggc tggcgtccaa
      181 tgcgatcccg cttcgaaccc tttccgtcac ggacgaggac ttcggcgacc tcgaaccgct
      241 gatggcggcc attggcgacg cgcgcgtcgt catgctcggc gaacccagcc atggggccgg
      301 atcggccttc gcggccaagg cgcggctcgt ccgcttcttt caccaacgca tgggcttcga
      361 tgtactcgcg tgggagtcgg ggcttttcga tgttcgcctc gcccaggccg cattggccga
      421 tgcgagtctg gatccctcga aggctgccca ggcggggata cttccggtct ggtcgcgagc
      481 cgcagaggtg cggccggtat tcgaatacgc acgcgccacg ctcgcgactt cgcggccact
      541 ggagttggtg ggattcgaca tcaatgcctc ggcgccgaag accaacgaaa agctcgccac
      601 cgaaatgcgc gcgtttttca ccgccgtgcg cgagccggca ttgcgcgatg gcggagaaaa
      661 acttgctgac gagttgatcg ctgcgttcaa gcgcgtccag gcacgcgacg agccaaagcc
      721 gggccagggc gatctcgaca acttgcatcg ggcggccgag cagttggacg cgctgctggt
      781 gcagcgtcga atcgacttca tgcaagttca cgcaatgcgt gatgtcgaac tcatgcagca
      841 cctggtcgcc aatctcgagt ctgcgggtac caacgcctgg catcgcaggt gctgcgaagc
      901 gcccaacggc gatgccgcca tccagttgca aactgcggag tggaatcgtc gagatctgag
      961 tttggcgacc aacctgcgct ggctgctcga aaagcgctac gcgggacgca aggcagtggt
     1021 atgggcgcac aacgctcatc tcatgcgtgc gtacttcgcc gcggattgga atggcgtgca
     1081 cgcaaagcct cagcgtaacg gaatgactcc catgggcgcg atcgttgccc aatggctgaa
     1141 ggatgatgtt tacaccatcg cgctgaccac ctacgaaggc gaggaagcct gggcgaatgg
     1201 acagaagcgc ggaaccatat cgcccgcgcc cgcgggaagc ctcgaggccc gcctgcatgc
     1261 gctcggcgag ccacacttgt tcatcgatct gcgccaggcg cgcggcgttc gctcacatcc
     1321 attgcgcagg ccaacgacgc tgcgaatcag cggttacggc cagccgacgg gcaagtacgg
     1381 caatgaatcc gtggcggata tcacgcaggc attcgatggc gtgctgttca tcgaccgcat
     1441 ggcgccggca acacgccttc cggattgagc ctgtcatgcc gcgggctggc acgcgcgaca
     1501 ccacgctcga gcccacctat cattcccgca tcgacgcggg agaagcgtgt gaaagattca
     1561 caggcaaacg gtgggaatgt gtcagtgcca gtcggcaggc gcggcttcgt cgccgcggcg
     1621 ggtgcgctga cgctcgcgcc ccgcatcttc gccgccaccg accgcacgga actactcatc
     1681 cgcacaccga tggcggcgcc cgaatgggcg ctgctgcagc gtgaactgct gaatgcgcat
     1741 accgaggcct gcgaaacttt ccacgccagg tatttcgacg agcgcaatca cctgctgtgt
     1801 ttcccgcggt ggggcacgaa cgacggcccg gacgacgcca tcgagcatgt gaacgactgg
     1861 ccactggtgc acgcactcgg tggttcgaat cgggtcctcg atctgtaccg cgccgtctat
     1921 gagggacaca tcgagcagta ctccgcgttg cgtaccaaga gcgtccccat gggacgccag
     1981 ggcatgtacg tgcgcgagtt ccctccgcag ctggactggc agcacatcag cgaagggctg
     2041 tccgtattca atctcatggg gctgtcgacg ccgacagaca cgcgtctaat cgaacgcgcg
     2101 cggcgcttcg ccggcttcta cgacggcagc gatccggctg cacccaacta cgaccgcaag
     2161 catcgcgtca tccgcagcat gttcaacgga agcctgggtc ccctgctccg cccgacgacc
     2221 gcgctcgact gggccggcga tcctttcgat gcttcgcgtt tcaaactgga acacggcgag
     2281 cgcaatttcg aggagttcct ggctcacttc caggaataca ccgacacggt ttgcgacagt
     2341 ccactcaacc tgcagtcgac cacgctcgcg ctcaatgcct ggctattgac cggcgaaaag
     2401 cgctggcgcg actggctgct gtcatacgtg gacgcctggg tcgaacgtgc gggcgcgaat
     2461 gatgacatcc tgccgagcaa ggtcgggctc gatggccgca tcgggaaatg gtacgagggc
     2521 gtttacggct gggccttcag ccccgtcgtg ccgcagaccg gcaagcgtga agatcgaaat
     2581 cgtgtcccgc gttccattgt cgcgttcatg aacgcggcgc tgctcacggg cgatgaccgc
     2641 tacatggccg tgtggcgccg gcagaacgcc gccatcaatt cgcacgcgaa agagatcgac
     2701 gggcaattgc agacgccgcg aatgtgcggc tccgcgggct ggtacagcta tgcgccgggc
     2761 ccgtatcgat tgaacggatt tgagatctgg tatctgtcga tgagcagtga ggatcgcgcg
     2821 cttgcgggca gtcatccgtg ggtcgatttt ctcgaggggc gtaatcccgg ctatccagtg
     2881 acggcgctgc gcgcctcgct cgaacgcctg cgcgcgcgtg tcgcgctcgt acaggccgac
     2941 aagtccactc ccgagacccg cctggccgac aacgcgctgg aatacaaccc ggcgagcgtg
     3001 gcttcgctgc tgcagctcac ttcaggcgcc ctgcatatcg cccgtccacc gtggtcgtcg
     3061 acctcgccta accagggcgg cgcgccgctg catgcgcgat tgcgctactt cgacccggtt
     3121 cgccgtcgcg ccggcctgcc ggaggacgtc ggcgcgctcg tggacaaact cgaagcccat
     3181 cgcaccgaag tcacgctggt gaacctccac cccaaccgcg agcgcgtggt cacggttcaa
     3241 ggtggcgcct atggcgaaca cgtcatcctg tcggcgcatg acgggagccg ggctcacgcc
     3301 gtcgacggcc gttcgttcga cgtaaggctg gcgcccggct gcggtgcacg gctcagcctc
     3361 atgatgcggc gctattcagc caaccctacg ctggcctttc cgtgggccta gctgatctgg
     3421 ttagttcagt ttctgggcgc gctcgatgag ctttcgcagc tcctcgactt ccgcgggctt
     3481 cagatccgtg agcggtgagc gcacatggcc cgccggccgt ccgacgatgc gcatgcccgc
     3541 cttcacgata gacaccgcat aacccttcgc acggttgcgg atcgcgatgt aaggcatcac
     3601 gaaatcgcgc agatgatgtt ggatcgttgc gcggtcgccg ccgcgcacgg cgccgaagaa
     3661 tctcttcgcc cacatcggca tgaagttgaa gatggccgac gaatacgtat cgaagccgat
     3721 gtcgtgatac ggcagcgcat aggtttccgc cgtgggcatg ccaccgatat aggtgacgcg
     3781 cttgccaagc cgcgagtaga cgcgcgcgag cagctcgaga tctccgacac cgtccttgaa
     3841 accgaccagg ttcgcattgc gctcacagag gcccgccagg gtctcttcgg tgacgatggc
     3901 gttatcccgg ttgtagacga tgacgccgat cgacacggcc ttgcagacct tttcgatgtg
     3961 cgccgcgagc cccgcctgct ccgagttcac cagatacggc ggcagcagca acaatccatc
     4021 ggcaccgcgt ttctcggcgt cgcgcgccag ttcgatggcc atcgccgttc catatccgca
     4081 gcccgcgatg acgggcacac ggcccttcgt ttcggcgacc gccgcactga cgacctcgcc
     4141 gacctcggcc ggcttcaggg aaaagaattc accggttccg cccgcggcga acagtcccgc
     4201 cgcctcgttc tccagcagcc acgagacatg tttcctgtag gcgtccgcgt cgaagcgatc
     4261 ttcggcatcg aagtgcgtga ccggaaagga cagcaggccg cgcgcgatgc gtgcggccat
     4321 atcggcaggt ttcattccca tggccgtgac cgtacccgtt gccctcaggc gtcgcaagag
     4381 atgggctaca gtccagtcca tcgtgctgga ccgtctaaaa cgctgggcaa aagccatcat
     4441 gctcgacgtg catgccctct acctcgcggc tcgcgatccg cgtgtgccgt ggctggcaaa
     4501 ggccgtggca atcctggtgg cgggttacgc gctctctccc atcgatctca tacctgactt
     4561 cattcccgtc ctgggatatc tcgatgatct gctcatcgtg ccgttgggaa tctggctggc
     4621 ggtgcgcatg attccacctg aagtcatggc cgagcatcgc gcaaccgcag aatcgtcggg
     4681 acgccgtccg accagcaagg caggcgcagt cgcgatagtc acgatctggg ttttgctggc
     4741 ggcggctgcc ggatgggcta cctaccggtt catttcccgt taaggcaatc tgtcaaacga
     4801 ccctggttca gttggcctta agccgggccc acgtagattg gatttccatg gaggtacgag
     4861 acatggaaac gctgaacgaa atgagcattg cggaatcgcg tcgggttttc gaactgtgcg
     4921 acctcaacgg cgacggcttc ctcgaccagg atgaattcca tgtcctgctg gaagcgctcg
     4981 atggcgacgt ctcacgcgcc gagtgcctgc tcgacttcga aattgccgac accgcgggcg
     5041 acggctatat cgaattcagg gaattcgtcg cgtggtggac aaactaggga gagccggccg
     5101 cctcaccaaa gatgaggaaa tcggtcacga gctgacagac cttgccatcc gcatcgaagc
     5161 cgaaaaagga cgggtacgcg ccgtcgccgt agccactcga gaaacagatg atgttctctt
     5221 cgcgttcttc gatcggccgc accacggccc aattgcgcgt gtgtcgatac gtcatctcca
     5281 gctcctcctc cagcaagtcc ccgaaggcga agtacggtcc ctcttgccga tagcgctggc
     5341 ccatcagcgc tgcagcggca aaatccagga a
//
LOCUS       DASQJS010000291        18778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1429055, whole genome shotgun sequence.
ACCESSION   DASQJS010000291 DASQJS010000000
VERSION     DASQJS010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18778
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429055"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..476
                     /locus_tag="VEW08_11640"
     CDS             321..476
                     /locus_tag="VEW08_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41436.1"
                     /translation="MTKKTCAACDYPLEPDSTIKVKIGGKTVEVCCEECATKLREAAT
                     AKQKKRA"
     gene            865..1380
                     /locus_tag="VEW08_11645"
     CDS             865..1380
                     /locus_tag="VEW08_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41437.1"
                     /translation="MSRHYHTLTHLDACLRELDDVRDLALRPAEVELALWLHDAVYRS
                     WRRDNEQRSADLAALTLQSASVESVERIRQMILATMHGEEAQGGDTALVTDIDLSILG
                     QPPDVYAVFERAIRREYWWVPRTRYVAGRRRVLEGFLARSTIYRHRRFHEKYEVQARA
                     NIAAALRNLAA"
     gene            1568..3415
                     /locus_tag="VEW08_11650"
     CDS             1568..3415
                     /locus_tag="VEW08_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HYJ41438.1"
                     /translation="MGAAALFAQPAAAQMAAGKAKFVGNVTGNSVPANFGTYWNQVTP
                     ENATKWGSVEGTQNQMNWAGADAAYNWAQTNGYKFKFHTFVWGSQFPGWLSGLSQAQQ
                     RAEIEQWITAACARYPNMWAVDVVNEPIKTALPWKAALGGDGATGWDWVITAFQLART
                     ACPNAKLLINEYGTENDPPVRNTYKTIINLLKTRGLIDGIGIQAHYFNIDNMNRAAVT
                     TMLNDYATLALDVWVSELDIVGPGGDSGQLAKYQDVFPGFWEHSAVKGITLWGYIVGQ
                     TWRDGTGLVTTGGVERPAMTWLKGYVSGSGGGDTQAPSVPANLTAPSTTSTSISLAWT
                     ASTDNVGVTGYQILRAPGASGGTFAQIATSTTNSYVNTGLTASTTFRYQVRAVDAAGN
                     ASAVSNTVTAATQPGGGGDVTPPTAPTNLTAPSTTTTSVSLAWTASTDNVGVTGYQIL
                     RAPGASGGTFTQIATSTTNSYVNNGLTANTTYRYQVRATDAVGLVSAVSNTVTAMTQT
                     GGTGGGCSVTPTVQTQWQTGYVIEPSRVTNTGTATINSWTVTFTLPAGHTVIGSWNAV
                     LTISGQTVTARNASHNGTVAAGGNTAFGFQVSRPNGNTMLPTGYTCATP"
     gene            3429..4142
                     /locus_tag="VEW08_11655"
     CDS             3429..4142
                     /locus_tag="VEW08_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41439.1"
                     /translation="MIDAGNRGNGGRRYALPAFFVLTTAVAIALTIAFVADDSQTPDG
                     DAMAALPGVSVSPVRPASLVTPHASRDRQAQLALEVERALVSNNPLERDKAMYFSLPE
                     LLDADPSRVIELVARQEGETRDALRDEVVRLWIRKDREAALIWMGSLENEWERNASAT
                     IAMRTLAAIDPAQAVAVADQFGIGRDDGSLEHIVQIWASENFEECTKWLETQPENAQT
                     AQLRARIDQVRKQRLAALE"
     gene            4338..4910
                     /locus_tag="VEW08_11660"
     CDS             4338..4910
                     /locus_tag="VEW08_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41440.1"
                     /translation="MRQISIVSLILVTLLGVGAAYWIDAAEKPRRHWPKPTPPPPPSP
                     IYTTSREHVQSQEEIAQQARDHNGLLELEIESALMGKDAQRREGAFTFLLPELVQVDP
                     PRVVAMVARQPPGEARDTLRTEVTRQWMARDPDAAVRWMKTLPEGERRESAAIAVESI
                     IGHSPIKAAALADEFGITGLLAHRLKAARD"
     gene            4972..6297
                     /locus_tag="VEW08_11665"
     CDS             4972..6297
                     /locus_tag="VEW08_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-mannosidase"
                     /protein_id="HYJ41441.1"
                     /translation="MLNHRWLAVLIAFLGSVSGAQAADAIAAQSGFVTVSRKHFVLDG
                     KPYYFAGANLWYGMYLGSPGKTGNRTRLLKELDQLAAQGVTNLRVLAISESSTLKRSV
                     TPAVVLRPNHIDETLWQGLDFLLAEMAKRDMKAVLYLNNFWQWSGGMSQYLAWFNGKP
                     VVDPDVTGNWNEFMDNSAAFYREPKAQAAFRFAVKQLITRKNSVNGRMYTADPTIMSW
                     QLANEPRPGSDANAKPYFDVFVKWIDETAGYIKALAPQQLVSTGNEGWMGTGGSRELF
                     VKSHSTKNVDYLTFHMWAPNWSWYDPKNSAATYDGAWVKMKEYLDWHIDSANKMVKPI
                     VLEEFGINRDEGSFRPTATTVYRDKFYAAIYGLLARRAAAGDAIAGSNFWAWGGAGRT
                     QNADFMWKAGDSFVGDPPQEAQGLYCVFDSDISTVKIISAHARQMSGLH"
     gene            complement(6257..8770)
                     /locus_tag="VEW08_11670"
     CDS             complement(6257..8770)
                     /locus_tag="VEW08_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ41442.1"
                     /translation="MNPDLKKQLAQAFGVGSEAELEALIAKLRAGHEVDPQLMRKGCL
                     ALVDNAREAHAAHATSDQRLKIALEASNLGLWEWDLTTDNLFVDRTYFGFLGIERDDQ
                     VMPINDLVSLVHPEDMDVFSNATRDALRGNVPMLQVQHRMRHADGRWVWLETYGNVSS
                     RDELGRATRMTGTHSNITERKQLERALSNTLRVLRALLETLPLPVILRDTERRVTLVN
                     AAFEKLFGIPREEIVGKPLHHDPNTPPVRGHRETDDQLLAEHKSLRYETSIQVANGSI
                     FDVIIAKTPLMAEDGTITGIASVITDISEQKRTAEVLEKARVTAEAAVQAKSRFLANM
                     SHELRTPLNGVVGMASLLENTALDAKQRRFVRTLKSSAEALVTLINDVLDLSKAEAGK
                     LALASGPFELRRELEQVVGLFGARAYDKGIEIAAHIARGVPATVHGDPIRLRQVLGNL
                     VNNAVKFTDSGAVLLAVTAVPAGAGPATLEFSVSDTGVGIAPAEQQRIFDAFEQADGS
                     VTRKFGGTGLGLAISRQLVELMRGTMELSSEPGRGSRFSFRIPMGVDPLELPPVMPAA
                     DLGAIVIGLHPIIRSAVCDALSAEVSHVISVDSPMTAIEALQDFSKRITRVRIVIDAN
                     AAAKIEHAVSGLRAAAAPRQVEVVVLMPPDADAAPPTGVTRVLVKPVCTVDFVATAAV
                     DTAQSTRIRALPISGSRGRALVVEDNAVNQEMARAMLDMLGFRVTTASNGREGVLAAA
                     ADPELDLILMDCQMPVMDGLAAARAIRAAETGGLRVPIVALTGNAMPGDREACVAAGM
                     DDYLAKPFSLTALRAMIDRWAGPGKPASEVHSSGARAPR"
     gene            complement(8921..9796)
                     /locus_tag="VEW08_11675"
     CDS             complement(8921..9796)
                     /locus_tag="VEW08_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41443.1"
                     /translation="MKKTAALMVALGLAAVFQFSATVPAQAQAQTTAHKLTRLWESEA
                     TLKVPEGVRLDAKRKVLYVSNIDGQPWEADGKGSIAKVGLDGKVISAEWVTGLDCPKG
                     LALSADGKWLYAADAGGIVVIDIKAGKIKNKIAIPEGVQLNDLASDGKDTLYVSDSKA
                     KKVYVVKDGKASVYLDETVLKGPNGLLVHGGTLYVLDNNSLNRVEPDKSLKVLADGMP
                     GGVDGLENVKGNDFIVSVWSGAVWYVSADGSKELMFDGKAVQTSTADIGWDPSTMTVY
                     VPTFFKNSIIAFKVE"
     gene            9868..10485
                     /locus_tag="VEW08_11680"
     CDS             9868..10485
                     /locus_tag="VEW08_11680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HYJ41444.1"
                     /translation="MPVSTLFATRVYTKSLKKGGSDPLNARLLRECRQLSLEDAAGIR
                     WSETNYPGGYTSYTSLSRMHQMSPTFAALEKRIDAHVRMFARTLDFDLTGRKLVMTDC
                     WVNVMTRQVVHGLHLHPLASLSGTYYVRTPRGCSGLKFEDPRLDRFMASPPRKARARR
                     ENQVWVTVPAEQGKVVLFESWLRHEVAPNPTEAERISISFNYSWF"
     gene            complement(10576..11181)
                     /locus_tag="VEW08_11685"
     CDS             complement(10576..11181)
                     /locus_tag="VEW08_11685"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYJ41445.1"
                     /translation="MNFDRTPGSMLILGAILVMSALALWVAPAIMERNLLRPYWLLRR
                     KQYDTIITSGFIHGDFGHLLFNSLTFYFFAPSLEHRIGTARFVALYFIGLVLSSLGTV
                     YKQRNNPDYAALGASGAILAVLFAYIVYYPKQMLYLYFAVPIPAVVFAFGYVAYSWWA
                     SKHKRDNINHDAHLDGALTGLIFVGLTDFPAWSRALHQIFG"
     gene            complement(11186..12049)
                     /locus_tag="VEW08_11690"
     CDS             complement(11186..12049)
                     /locus_tag="VEW08_11690"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ41446.1"
                     /translation="MPTTRFLPHIFLLALCGAAHAQQPAALTVIPLWEKGAPGFESRR
                     NEPEQHQDWWYKNIHNPSLTVFLPPNGKSNGTAVIVAAGGGHRELVFNPEGVEPAQYL
                     ASLGVTAFALKYRLFREPGSKYTIDNTAEDIRRAMRTVRARAAEWHIDPNRIGVMGWS
                     AGGEVAALVAYPPVAGDPKSQDPVEWVSARPDFQILIYPGPLGIPAKVTRDAPPLFLS
                     GAADDEYVAPVLFDLARKYHDAGASIEAHIYAQGKHAFNMGQRSQFVSIQRWPQRLAE
                     WLEDRKLTKAH"
     gene            complement(12625..14742)
                     /locus_tag="VEW08_11695"
     CDS             complement(12625..14742)
                     /locus_tag="VEW08_11695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ41447.1"
                     /translation="MGRISDLIRIGPISGFRLRTKLLLLALTTLALPWAGCQYAREME
                     NVLRESEKQSLLAVTTTIAGSLKGRQELLFRDDSLPAVSDGNARDITPIVLSGAPLID
                     GRADEWDTDSRNLTRVAGPGGDSLQLLAATHERWLYLALLVRDNKLVYDASMLAPLDS
                     ATIGDRIWLAFDDKRGGQSQLFFGSTGGGRLVGRRIETREYGREEAVAEPRIEAVWQR
                     SRDGYVLELGIPLSLLGQHIGVLIDDRDRRGATRASYGTLEVSDLRATGRLIAASPDL
                     NEHLRQFAQPGVELTVASSTGAILTRIDAPALPGDYTRMRGFLPRMYRLFLDGDAIPR
                     GVSQAERQRAAESLTSRVAQGKPETALFAGRYENSVIVAAAAPIYSADGKNVFGVIQL
                     AQTADRWLTLRDTALTRLLNLTLFVTLFAVVAAFWFAGRMTLRISRLGAASETALGRE
                     GNLSRVLPEADARDELGDLSRSFSSLLGRLDEYTGYLRSLAGKLAHEIRTPLTIIRSS
                     LENLESEGDSANAKVYISRAREGSERLGAILTAMGAATRVEEAIAHSDRLRFDLAALV
                     RAAAGAYGGAFPARRFAAQVPDEAVEITGAPDLIVQMLDKLVDNAVDFSVDGSTISIV
                     LRAEPDYVELSVANSGPLLPSETGNRLFESLWQSRVESDKRPHFGLGLYIVRLISEFH
                     GGTARAENLPDNSGAIFTVRLNR"
     gene            complement(14750..15439)
                     /gene="pdsR"
                     /locus_tag="VEW08_11700"
     CDS             complement(14750..15439)
                     /gene="pdsR"
                     /locus_tag="VEW08_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HYJ41448.1"
                     /translation="MKKTIAIVEDERAIRDNYAAAFSREGYAVRGYPNRREAMAAFAA
                     RLPDLAVIDISLEDEPEGGFELCRELRGLSPELPIIFLTARDSEIDAVSGLRLGADDF
                     LTKDLSLAHLIARVNALFRRVDALRRPTEEKQVIRRGALSLDSERMSVEWNGQVVLLS
                     LTEFWIVYALTKHPGHVKNRQQLMDAANVVLDDNTITSHIKRIRRKFQVVDEKFDAVH
                     TVYGMGYRWVE"
     gene            15588..15779
                     /locus_tag="VEW08_11705"
     CDS             15588..15779
                     /locus_tag="VEW08_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41449.1"
                     /translation="MTDQPNAFDRTYAAEQFATQQGERWRRFLERVGTRSNDFAARLA
                     RARFVYLHRRETATHWLPL"
     gene            15800..15940
                     /locus_tag="VEW08_11710"
     CDS             15800..15940
                     /locus_tag="VEW08_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41450.1"
                     /translation="MNEKPETRGPVAEENDSEASFWHWFALGALLFAWIAEATMCAER
                     GF"
     gene            complement(15950..16339)
                     /locus_tag="VEW08_11715"
     CDS             complement(15950..16339)
                     /locus_tag="VEW08_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYJ41451.1"
                     /translation="MPGHSSVAEPQLNATPLIDVMLVLLVILIITLPVATHAVKLNLP
                     QGPAMAPQPSIRLDIAFDGAIYWNGAEVTSLEQLGERFAAVAGSANEPRINVVPERRT
                     RYEVVAQVLALAQRSRVTRLGLAPMPD"
     gene            16397..16684
                     /locus_tag="VEW08_11720"
     CDS             16397..16684
                     /locus_tag="VEW08_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41452.1"
                     /translation="MRAEPRYKALPLEAATLLSEGNVIEAIKVVRKTEGAGRREAKAR
                     VDAHIAQDPILQVQLETQQRARRRKFFFWFVVVDLAITAGIIYWLFYRGSA"
     gene            16684..17322
                     /locus_tag="VEW08_11725"
     CDS             16684..17322
                     /locus_tag="VEW08_11725"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYJ41453.1"
                     /translation="MLLIGMFDSPFVRRVAVSMKLLDIPFEHGNWSVGKDFDRIRNYN
                     PLGRVPTLVTDTGESLMESGAILDYLDELAGPERALLPRSGAERRKALHLMAMATGAA
                     EKGLLQIYERIFRPETKRHQPWVDRCRLQMSSSLAAIDRYLGERGVAQWLVGKRMTQA
                     DITAVCAFTFLNDALRVADDAVMFQSLATLASRCESMPVFQETRVPFHAPNS"
     gene            complement(17369..18526)
                     /locus_tag="VEW08_11730"
     CDS             complement(17369..18526)
                     /locus_tag="VEW08_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008002122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ41454.1"
                     /translation="MLNPLDLYDIRGLLTEDERMVQDSVGRFVDEKVLPVIQECFEKH
                     HFPKELIPELAELGLLGSSIQGYDCAGLNAVSYGLICQELERGDSGIRSFVSVQSSLC
                     MYPIFAFGSDEQKQKWLPDMARGRKIGCFGLTEPHGGSDPSNMKTHAKKKGGDWVING
                     SKMWITNGAIADLCICWAMTDDGIRGFIIEKGMKGFAAPEIERKFSLRASVTSSLFFD
                     NVVVPESNLLPGVKGLKGPLSCLTQARYGITWGVIGAAQACLAQLLDYTKTRVMFGKP
                     LAATQAIQLRLAEMARKITTAQLLSHQLGRLKDQGKMQPAQVSLAKWNNCRIALDIAR
                     DCRDMLGGSGISAEYVPIRHALNLESVITYEGTETIHQLTIGRELTGISAF"
     gene            complement(18608..18775)
                     /locus_tag="VEW08_11735"
     CDS             complement(18608..18775)
                     /locus_tag="VEW08_11735"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HYJ41455.1"
                     /translation="MWLTCLVIFAIGWVGQFIGHAIEGKRPSFFKDLQFLLIGPLWLL
                     GHLYRRLGIGY"
BASE COUNT         3520 a         5814 c         6016 g         3428 t
ORIGIN      
        1 ggcctcgtta ggcacgaagc cgatccctgc gacatacgga ctctttgctg ccgcccaact
       61 caactgatta caagatgtct tcgcgtgtgt cggcggcccg caaactagcg atgtcaccct
      121 gaacgaaggg cccggcgaaa agcgagtctc atcgcatgca gggccacccg tcgctcgacc
      181 ctccacccac gaaccccgtc ccgatttgcc agcaaatcag gaatgtcccc attcatcact
      241 aaagatttcg acagcgcccc cgcgccccag atggtctcct gagcacgccc cgaaacgagg
      301 gtgaacctga ggattccgcc atgaccaaga aaacctgtgc ggcctgcgac tacccactgg
      361 aaccggattc caccatcaag gtgaaaatcg gcggcaagac ggtcgaagtc tgttgcgagg
      421 aatgcgcgac caagttgcgg gaggcggcga cggcgaagca gaaaaagcgt gcgtgaggat
      481 taagggggca tttctgtcgc gctgccaaaa cggtaagaag tctcggtttg cgcgtgtgcg
      541 cgccgccacc cgattgctgg cgcggcgccc acgcgcccag actgacgccg cgatatacca
      601 acaagcggtc cgaaacgctc gcaagaagcg ccgggccgca acatcagcgg aacaccgggg
      661 acctttcgaa gtgacgtcga tttcctattt gcccaccacg ttcacgacgc ttttcgttct
      721 cgccgccgga cttctgctgg gctacatcca gtaagcgatc ccactccaag ggtgaatttc
      781 gagcgctgga gtcgcgcctg tatcgatatt ggcgcggcgc ccgacgccag agcctaccgg
      841 cgtattcgcc gctcatggag tggcatgagt cgccactacc acacgctcac gcatctcgat
      901 gcctgcctgc gcgaactgga cgacgtgcgc gacctggctc tgcgtccagc ggaagtcgag
      961 ctggcgctgt ggctgcacga cgccgtgtat cgaagctggc gccgcgacaa cgagcaacgt
     1021 agcgccgacc ttgcggcatt gaccctgcaa tccgcgtccg tcgagtcggt ggagcgcatc
     1081 cgccagatga tcctcgccac catgcatggg gaagaagcgc agggcggcga cacggcgctg
     1141 gtcaccgaca tcgacctttc gattctcggc cagcctccgg atgtttacgc cgtgttcgag
     1201 cgcgccatcc ggcgcgaata ctggtgggtg ccgcgcacac gatacgtggc ggggcggcgg
     1261 cgcgtgctcg aagggtttct cgcgcgctcg actatctacc gccaccgccg cttccacgaa
     1321 aagtacgaag tccaggcccg cgcgaatatc gcggcggcac tgcggaatct cgccgcatag
     1381 cagcaagcgg ctgacggttt accgaagttg cgttaacgct aacaagcagc gtatgttcgc
     1441 cccgcgtccg ggatgacgca tggagcgggc gcaaaaaggg acttttcgac aaaccgttca
     1501 cgcaggggga ctctcaatga gcattcgcac ggccgcacgc ggccgactcc tttctattgc
     1561 tttgattttg ggcgctgcag cacttttcgc gcagcctgct gccgcgcaga tggcggctgg
     1621 caaggcgaag ttcgtcggca acgtcaccgg taacagcgtg ccggcgaatt tcggcacgta
     1681 ctggaaccag gtaactccgg aaaacgcgac gaagtggggt tccgtcgagg gcacgcagaa
     1741 ccagatgaac tgggcaggcg ccgacgcggc ctacaactgg gcgcaaacca acggctacaa
     1801 gttcaagttc catacgttcg tgtggggttc gcagttcccc ggctggctca gtggtctctc
     1861 gcaggcgcag cagcgcgccg agatcgaaca gtggatcacg gccgcgtgtg cgcgttatcc
     1921 gaacatgtgg gccgtggacg tggtcaacga gccaatcaag accgcgctgc catggaaagc
     1981 tgcgctcggc ggcgatggcg ccacaggctg ggactgggtg atcaccgcgt tccagctcgc
     2041 acgcaccgcg tgcccgaacg ccaaactact catcaacgag tacggtacgg aaaacgatcc
     2101 gccggtgcgc aacacctaca agaccatcat caacctgttg aaaacccgcg ggctgatcga
     2161 tggcatcggc atccaggcgc actacttcaa catcgacaac atgaaccgcg ccgcggtcac
     2221 gacgatgttg aacgactacg cgacgctggc gctcgacgtg tgggtgtcgg aactcgacat
     2281 cgtcggcccc ggtggcgatt cgggtcagct cgcgaaatac caggatgtgt tccccggctt
     2341 ctgggagcat tccgcggtca aaggcatcac gctctggggc tacatcgtcg gccagacctg
     2401 gcgcgatggc accggtctcg tcaccacggg cggagttgaa cgtcccgcca tgacctggct
     2461 caagggctac gtcagcggtt cgggcggtgg tgatacgcag gcgcccagcg tgcccgcgaa
     2521 tctcacggca ccgtcgacga cctcgacttc aatatcgctg gcgtggactg cgtcgacgga
     2581 caacgtcggt gtgacgggtt atcagatcct gcgcgcacct ggcgcaagcg gtggcacgtt
     2641 cgcgcaaatc gccacctcga cgaccaactc ttacgtcaac acggggctca ccgcgagcac
     2701 cacgttccgc taccaggtac gggctgtcga tgccgcgggc aacgcttcgg cggtgtcgaa
     2761 tacggtcacc gcggcgacgc aacccggtgg cggcggcgat gtgacgccgc cgactgcgcc
     2821 gaccaacctg acggcaccgt caacgacgac gacctcggtg tcgctggcgt ggacggcatc
     2881 gacggacaac gtcggcgtga cggggtatca gatcctgcgt gcgccaggcg ccagtggcgg
     2941 tacgttcacg cagatcgcca catcgaccac caactcctac gtcaacaacg gcctcacggc
     3001 gaacacgact taccgttacc aggtgcgagc caccgacgca gtgggccttg tttcagcggt
     3061 atcgaatacc gtgaccgcga tgacgcagac cggtgggacc ggcggcgggt gttcggtgac
     3121 cccgaccgtg cagacgcagt ggcagacggg ctatgtcatc gaaccgtccc gcgtgaccaa
     3181 tacgggaacg gccacgatca atagctggac ggtcacgttc acactgccgg caggacatac
     3241 cgtgatcgga tcgtggaacg ccgtactcac catcagcgga cagaccgtca cggcgcgcaa
     3301 cgcgtctcac aacggaacgg ttgccgcggg cgggaatacc gcgttcggct tccaggtgag
     3361 ccgtccgaac ggcaacacga tgctgccgac cggctacacc tgcgcaacgc cttgagattg
     3421 agggggacgt gattgacgcg ggcaatcggg gaaacggcgg gcggcgctat gcgctgcccg
     3481 ccttcttcgt gctgacaacg gccgtggcaa ttgcgctcac cattgctttc gtagcggacg
     3541 actcgcaaac gcctgatggc gatgccatgg cagcgcttcc gggtgtctcg gtgtcaccgg
     3601 tgagaccggc atccctcgtg acgccacatg catcgcgcga tcggcaggcg cagttggcgc
     3661 tcgaagtcga acgtgcgctg gtgtctaaca acccgctgga acgcgacaag gcgatgtact
     3721 tctcattgcc cgagctgctc gacgcggacc cgtcgcgtgt catcgagctg gtggcgcgac
     3781 aggaaggcga gacgcgagac gcgcttcgcg atgaggtggt gcgcctctgg atacgcaagg
     3841 atcgtgaggc agcgttgatc tggatgggat cgctcgagaa cgagtgggag cgcaacgcga
     3901 gcgcaaccat cgcgatgcga acgctggcgg ctatcgatcc ggcgcaggcc gttgccgtcg
     3961 ccgaccagtt cggcatcggg cgcgacgacg gctcgctcga gcacatcgtg cagatctggg
     4021 cctcggaaaa tttcgaggag tgtacgaagt ggctcgaaac acagcctgaa aacgcgcaaa
     4081 cggcgcaatt gcgcgcgcgc atcgatcagg tgcgcaaaca acgtttggcg gcgcttgaat
     4141 gattgcggca ggtacgtata acttccgcgt tcgcaacgga acaccatcag ctgattgacg
     4201 acggtgacac cgtgtaagag atttctgacg actgaatgcg ataccccgtc aggcaggtat
     4261 ggcaaggcag gcaactgccg agcgtaccgg gcctgagtaa actcgcgggc agcgaccaaa
     4321 cgctgcccgt tttttttatg cggcaaatct ccatcgtctc tctcatcctg gtcacgctgc
     4381 tgggcgtggg ggcggcctat tggatcgatg ccgcggaaaa gccgcgccgg cactggccca
     4441 agccaacacc cccgccgcca ccttcgccta tctacacaac atcgcgtgaa cacgtgcaga
     4501 gccaggagga gatcgcgcag caggcgcgcg atcacaacgg actgctcgag ttggaaatcg
     4561 aaagcgcgct gatgggaaag gatgcgcagc ggcgcgaagg cgccttcacg ttcctgctgc
     4621 cggagctggt gcaagtggat cccccgcgcg tcgtcgcgat ggtggcgcga cagccgccgg
     4681 gcgaggcgcg cgacacgctc cgtaccgaag tcacgcgcca gtggatggcc agggatccgg
     4741 acgcggcggt ccggtggatg aagaccttgc cggaaggcga gcgccgtgaa agcgcagcca
     4801 tcgcggtcga atccatcatc gggcacagcc ccatcaaagc cgccgcactg gctgacgaat
     4861 tcgggattac cggcctcctg gctcacaggc tcaaagccgc ccgcgattga ctctcccctc
     4921 gaacggcacg ttagtatcgc gccgttcgct tttcatgagg ggtgaaggtc catgctgaat
     4981 caccgctggc ttgccgtatt gatcgctttt ctgggctccg tctccggagc gcaggcggcc
     5041 gacgccatcg ccgcgcaatc cggcttcgtc acggtatcgc gcaagcattt tgttctcgac
     5101 ggcaagcctt actacttcgc cggcgcaaat ctctggtacg gcatgtacct cggttctccc
     5161 ggcaagaccg gcaatcgtac gcggctgctc aaggagctgg accagctcgc cgcgcaaggg
     5221 gtcacgaatc tgcgcgtgct ggccatctcc gagtccagca cactgaagcg ttcggtgacg
     5281 ccggcggtgg tcctgcggcc gaatcacatc gacgaaaccc tgtggcaggg gctcgatttc
     5341 ctgctcgccg aaatggcgaa acgcgacatg aaagccgtgc tgtacctcaa caacttctgg
     5401 cagtggtcgg gcggcatgtc gcaatacctg gcgtggttca acggcaagcc ggtcgtggat
     5461 ccggacgtga ccggcaactg gaacgaattc atggacaact cggccgcgtt ctaccgggaa
     5521 cccaaggcgc aggcggcatt ccgctttgcc gtcaaacaac tcatcacgcg caaaaattcg
     5581 gtgaacggcc gcatgtacac cgcggatccg acgatcatgt cctggcagct cgccaacgaa
     5641 ccgcgtccgg gcagcgatgc caatgcgaaa ccgtacttcg acgtgttcgt gaaatggatc
     5701 gacgagacgg cgggctacat caaggcgctc gcgccgcaac agctcgtgag caccggcaac
     5761 gaaggctgga tgggtacggg tggtagccgc gagctgttcg tgaaatcgca ttccacgaaa
     5821 aatgtggact acctgacctt ccacatgtgg gcgccgaact ggagttggta cgacccgaag
     5881 aattccgcgg cgacgtacga cggcgcgtgg gtgaagatga aggaatatct cgactggcac
     5941 atcgactcgg ccaacaagat ggtcaagccc atcgtgctcg aggaattcgg catcaaccgc
     6001 gacgagggtt cattccggcc gaccgccacc acggtatacc gcgacaagtt ctacgccgcg
     6061 atctacgggt tgctcgcgcg ccgcgcggcg gcgggtgatg ccatcgccgg ctccaacttc
     6121 tgggcgtggg gcggcgcggg ccggacgcag aatgcggatt tcatgtggaa ggcgggcgac
     6181 agtttcgtcg gcgatccgcc gcaggaagcg cagggcctct actgcgtttt cgattcggac
     6241 atctcgacgg tgaaaatcat ctcggcgcac gcgcgccaga tgagtggact tcactagcgg
     6301 gtttaccggg acccgcccag cggtcgatca tcgcgcgcag ggcggtcagc gagaacggct
     6361 tggcaagata gtcgtccatg cccgcggcca cgcaggcttc acgatcgccg ggcatcgcat
     6421 tgccggtgag cgcgacgatg ggcacgcgca ggccgcccgt ctccgccgcg cggatcgcgc
     6481 gcgccgcagc caggccatcc atcactggca tctggcagtc catcaggatg aggtcgagct
     6541 cgggatcggc agccgcggca agcacacctt cacggccatt ggatgcggtg gtgacgcgga
     6601 agcccagcat gtcgagcatg gcgcgcgcca tttcctggtt cacggcgttg tcttcgacca
     6661 cgagtgcgcg gccgcgtgag ccggatatgg gcaacgcacg aatccgcgtg ctctgggcgg
     6721 tatccaccgc cgccgtcgca acgaagtcga cggtgcacac cggtttcacg agtacgcgcg
     6781 tgacgcccgt gggcggggcc gcatcggcat ccgggggcat caggaccacg acttccacct
     6841 ggcggggcgc ggccgcggcg cgcagcccgc tgaccgcgtg ttcgattttc gccgcggcgt
     6901 tggcgtcgat gacgatgcgc acccgcgtga ttcgcttcga gaaatcctgc agtgcttcga
     6961 tggcggtcat cggtgaatcg acgctgatca cgtgcgaaac ttccgccgat aacgcgtcgc
     7021 agaccgcgct gcggatgatc gggtgcaggc cgatgacgat tgcgcccagg tcggctgccg
     7081 gcatgaccgg cggtagttcg agcggatcca cgcccatcgg aatgcggaac gagaagcggc
     7141 tgccgcggcc gggttcactg ctgagctcca tggtgccgcg catgagctcg acgagctggc
     7201 ggctgatggc caggcccaat cccgtgccgc cgaatttgcg cgtcacgctg ccgtcggcct
     7261 gctcgaatgc atcgaaaatc ctttgctgct cggcgggcgc gatgccgacg ccggtatcgc
     7321 tgaccgagaa ttcgagcgtt gccggcccag cgcccgcggg aaccgcggtc acggccaaca
     7381 acacggcgcc cgagtcggtg aatttgacgg cgttgttcac cagattgccg agcacctggc
     7441 gcaaacggat ggggtcgcca tgcaccgtcg ccggtacgcc gcgtgcaatg tgcgcggcga
     7501 tttcgatgcc cttgtcgtag gcacgcgcgc caaacaggcc gaccacctgt tcgagctcgc
     7561 gtcgcagttc gaacgggccg ctggcgagcg cgagcttgcc ggcctcggcc ttggaaagat
     7621 ccagcacgtc gttgatgagc gtgacgagcg cctcggccga agacttcagc gtgcgtacga
     7681 agcggcgctg cttggcatcg agcgcggtgt tttcgagtag cgaggccatg ccaaccacgc
     7741 cattgagcgg cgtgcgcagc tcatggctca tgttggcgag gaagcgcgac tttgcctgca
     7801 cggcggcctc ggcggtcacg cgcgccttct cgagaacttc ggcggtgcgc ttctgctcgg
     7861 agatgtcggt gatcaccgag gcgatgccgg tgatggtgcc gtcctcggcc atcaacggcg
     7921 tcttggcgat gatcacatcg aagatggatc cgttcgcgac ctggatcgaa gtttcatagc
     7981 gcagcgactt gtgctcggcg agcagctggt cgtcggtttc gcggtggccg cgcactggcg
     8041 gggtattggg gtcgtggtga agcggcttgc cgacgatttc ctcgcgcgga atgccgaaca
     8101 gcttttcaaa cgcggcgttt accagcgtga cgcgcctctc ggtgtcgcgc aggatcacgg
     8161 gcagcggcag cgtttcgagc agcgcgcgca acacgcgcaa cgtgttcgac agcgcgcgtt
     8221 cgagttgttt gcgctcggtg atgttcgagt gcgtgcccgt catgcgcgtg gcacggccga
     8281 gctcatcgcg gctgctgacg tttccgtacg tctctagcca cacccaccgc ccgtcggcgt
     8341 gccgcatgcg atgctggacc tggagcatcg gaacgttgcc gcgcagtgcg tcgcgcgtgg
     8401 cattgctgaa cacatccatg tcttccggat gcaccaggct aaccagatca ttgatgggca
     8461 tgacctggtc atcgcgctcg attcccagaa agccgaaata cgtgcggtcg acgaacaagt
     8521 tgtcagtcgt gagatcccac tcccaaagcc cgaggttcga ggcttcgagg gcaatcttca
     8581 ggcgttgatc gctggtggcg tgcgccgcgt gcgcttcgcg cgcgttgtcg accagcgcga
     8641 ggcagccttt gcgcatgagt tgcggatcga cctcgtggcc cgcgcgcagt ttcgcgatca
     8701 gggcttcgag ttcggcttcc gagccgacac cgaaggcctg cgccagttgt ttcttgagat
     8761 cggggttcac ctgatatacg tgcgcagggc gcaacctcgg aagcttcact cctgctaacc
     8821 atgtcggcag cgggagagat tactgaggtt cggcggccag cggcatcagg cttgacccgg
     8881 ccggagtgtg tagccgttca cactgccggc cggtttatgg tcattcgact ttgaaggcga
     8941 tgatcgagtt cttgaagaac gtgggcacgt agaccgtcat ggtcgacggg tcccagccga
     9001 tgtcggcggt gctggtctgc acggccttgc catcgaacat cagttccttg gatccgtcgg
     9061 cgctcacgta ccacaccgcg ccgctccaca cggacacgat gaaatcgttg cccttgacgt
     9121 tttcgaggcc gtcgacgccc ccgggcatgc catcggcgag caccttgagc gacttgtccg
     9181 gctcgacgcg attgaggctg ttgttgtcga gcacgtacag cgtgccgcca tggactaaca
     9241 acccgttcgg gcccttgagc accgtctcgt cgaggtacac ggatgccttg ccgtccttca
     9301 cgacgtagac cttttttgcc ttggagtcgg acacgtacag cgtgtccttg ccgtcgctgg
     9361 cgaggtcgtt gagctgcaca ccttcaggga tggcgatctt gttcttgatc ttgccggcct
     9421 tgatgtcgat gaccacgatg ccgccggcgt cggccgcgta cagccacttg ccgtccgccg
     9481 agagcgcgag tcccttcggg caatcgagcc ccgtgaccca ctcggccgaa atgacttttc
     9541 cgtcgagccc tactttggcg atggagccct tgccatcggc ctcccacggc tggccgtcga
     9601 tgttggagac gtacagcacc ttgcgctttg cgtcgaggcg cacgccttcg gggaccttga
     9661 gcgtcgcttc ggattcccag agcctggtga gcttgtgagc cgtagtttga gcctgggcct
     9721 gggccggaac agtggccgag aattggaaaa cagccgcgag tcccaacgcg accataagtg
     9781 cagcagtctt tttcatgggc aaatgtcctt gatggaaggg gcgtcgatgg aaggacgcaa
     9841 agtatagatg cgcgttatgc tcaccgcatg cccgtgagca ccttgtttgc cacccgtgtc
     9901 tacaccaaaa gtcttaagaa gggcggttcg gacccgctga atgcgcggtt gttgcgcgag
     9961 tgccgccagc tcagtctcga agacgccgcc ggcatccgct ggtccgagac taactatccc
    10021 ggcgggtaca cgtcctacac ctctctctcc cgcatgcatc agatgtcgcc gacattcgcg
    10081 gcgctcgaga agcgcatcga cgcgcatgtg aggatgtttg cgcggacgct cgacttcgat
    10141 ctcaccggcc gcaagctcgt gatgacggat tgctgggtga acgtgatgac gcgccaggtg
    10201 gtgcatggcc tgcacctgca cccgctggct tcattgagcg gcacctacta tgtgcgcacg
    10261 ccgcgcggct gctccggact caagttcgaa gacccgaggc tcgaccgctt catggcttcg
    10321 ccaccgcgca aggcgcgtgc ccgccgtgaa aaccaggtgt gggttacggt gccggccgag
    10381 cagggaaagg tggtgttgtt cgaaagctgg ctgcgccacg aagtggcgcc gaaccccacc
    10441 gaggcggagc gcatttcgat cagcttcaac tattcgtggt tttgaacggt gccgggccgg
    10501 attggagtcc aacagtagcg acgcaccagg ttcggtggat gagttttccc ggtttcccgg
    10561 cccggcaccg tggttctagc cgaagatctg atggagcgcg cggctccacg ccggaaagtc
    10621 ggtgagcccg acgaagatga gcccggtcaa tgcgccgtcg agatgcgcgt cgtggttgat
    10681 gttgtcgcgc ttgtgcttcg aagcccacca gctgtacgcg acgtaaccga acgcgaagac
    10741 caccgcggga atcggcacgg cgaagtacag atacagcatc tgtttcgggt agtagacgat
    10801 gtaggcaaac aacaccgcga ggatggcgcc ggaagcgccg agcgcggcgt aatcaggatt
    10861 gttgcgctgc ttgtagaccg tgccgaggct gctcaggacc aggccgatga agtacaacgc
    10921 gacgaatcgc gccgtgccga tgcgatgctc cagggacggc gcaaagaagt agaacgtcag
    10981 cgaattgaac agcaggtggc cgaagtcgcc atggatgaag ccgctggtga tgatggtgtc
    11041 gtattgcttt cgccggagca gccagtatgg ccgcagcaga ttgcgttcca tgatcgcggg
    11101 cgctacccac agcgcgagcg cgctcatcac caggattgcg ccaaggatca gcatcgagcc
    11161 cggagttctg tcgaaattca tgtgatcaat gcgccttggt cagtttgcga tcctcgagcc
    11221 attcggccag gcgctgcggc cagcgctgga tgctcacgaa ttgcgatcgc tgccccatgt
    11281 tgaaggcatg tttgccctgc gcgtagatgt gcgcctcgat ggacgcgccg gcgtcgtggt
    11341 atttgcgcgc gagatcgaag agcacgggag ccacgtactc gtcgtcggca gctcctgaca
    11401 ggaacagcgg cggcgcatcg cgggtcacct tggcgggaat gccgagcggt cccggataga
    11461 tgagtatctg gaagtcggga cgtgcgctca cccattcgac gggatcctgc gatttcggat
    11521 cgccggccac tggtggatag gccaccagcg cggcgacttc gccgccggcc gaccagccca
    11581 tgacgccgat gcgatttgga tcgatgtgcc attcggcggc acgagcacgc accgtgcgca
    11641 tggcgcggcg gatgtcctcg gcggtgttgt cgatcgtgta tttcgagccg ggctcgcgaa
    11701 acaggcggta tttcagcgcg aacgccgtga cacccaggct cgccaggtat tgcgccggct
    11761 cgacaccctc gggattgaat acgagctcgc gatgcccgcc gccggcggcg acgatgactg
    11821 ccgtgccatt ggatttgccg ttgggtggca ggaacaccgt gagcgaagga ttgtggatgt
    11881 tcttgtacca ccagtcctgg tgctgctcgg gctcgttgcg ccgcgattca aaacccggcg
    11941 cacccttttc ccacaatgga atcaccgtca atgcggcggg ttgctgggcg tgtgccgcgc
    12001 cgcagagagc aagcaggaag atgtgtggaa gaaagcgggt agttggcatg ggccggcacc
    12061 ttgccacaac tggtggctca gcgtcttcgc acgaagcgat gacgtcgccc acgatcacta
    12121 acaacattcc aaaaaaaagc cccgcaaagc ggggctgtta gttgatcgtc gtcgacttag
    12181 gtaatcgctg tcagtcgatc gcgtcgacta cacggactgc tatattttcg gctgggacgt
    12241 tggcggcctt tcggcctccg gctaaacaat taagcccgcg tgtcagtcca cgctttgatc
    12301 gtcctcgcca gtagccaaaa ctaaaactac cgactgaacc cgtgcccgtg agcaaatccc
    12361 gagtttttct gtatcgctaa accgttgtgt tctccgcgca tcaataagtg gtgcagtgca
    12421 gaaccaataa tctttcgtat ttctttcaac ccgactaaca accttgtatt caggcactta
    12481 gctgtggttt cgagactacg cgagaccgga tcatacgccg aatctttccc gccacaatgt
    12541 ctcagtggcg cgacagagcg gctttctgtg aactgtatcg acagaattta cgaggactgg
    12601 atgtgactgg atgcgcgaat ccagtcatcg attgaggcgc acggtaaaaa tcgccccgga
    12661 attgtcggga aggttctcgg cccgggcggt gccgccgtgg aattcggaaa tcaggcgcac
    12721 gatgtacagc ccgagaccga agtgcgggcg cttgtcgctc tcgacgcgcg attgccacag
    12781 tgactcgaac agccggttgc cggtttccga cgggagaagc gggcccgaat tggccaccga
    12841 gagctccacg taatcgggct ccgcgcgcag aacaatcgat atcgtggagc catccacgct
    12901 gaaatcgacg gcattgtcca ctaacttgtc gagcatctga acgatgaggt ccggagcgcc
    12961 cgtgatctcg acggcttcgt cggggacttg ggccgcaaag cgtcgggcag ggaacgcgcc
    13021 gccgtaggca cccgcggccg ctcggaccag cgccgccaga tcgaagcgta gccggtcact
    13081 gtgggcgatg gcttcttcga cccgggtggc ggcgcccatg gcggtcagga tcgcgccgag
    13141 ccgctcgctg ccttcgcggg cgcgcgagat atagaccttg gcattggcgg agtccccctc
    13201 ggactccagg ttctcgagcg aagaacggat gatggtcagc ggcgtgcgga tttcgtgggc
    13261 cagcttgccg gccaggcttc gcaggtaccc ggtgtactca tcgagccgtc cgagcagtga
    13321 cgagaagctg cgcgacagat cgccgagctc gtcgcgggcg tcggcttcgg gcagaacgcg
    13381 cgacagattg ccctcgcgac cgagggccgt ttcgctcgcc gccccgagcc gcgagatacg
    13441 cagggtcatg cgaccggcga accagaaagc cgccaccacc gcgaacagcg tgacgaacag
    13501 cgtgagattc agcaggcgcg tgagcgcggt atcgcgcagc gtcagccagc gatccgcggt
    13561 ctgggcgagc tgaatgacgc cgaacacgtt tttgccgtcg gccgagtaga tgggtgcggc
    13621 cgccgcgacg atgacgctgt tttcatagcg gccggcgaac agcgcggttt ccggcttgcc
    13681 ctgcgccacg cgcgaggtga ggctttcagc cgcgcgctgc cgctcggcct gcgagactcc
    13741 acgtggaatt gcgtcgccat ccaggaacag gcggtacatc ctcggcagaa atccgcgcat
    13801 gcgcgtgtag tcgcccggca gcgcgggtgc gtcgattcgc gtaagtatcg cgccggtcga
    13861 cgaggccacc gtgagttcga cgccgggttg cgcaaactgg cgcaggtgtt cgttgaggtc
    13921 cggggatgca gcgatgagcc tgccagtggc tcgcagatcc gacacctcga gcgtcccgta
    13981 gctcgcgcgc gtcgcgccgc gccggtcgcg gtcgtcgatg agcacgccga tgtgctgccc
    14041 caacagcgac aacggaatgc cgagttcgag cacatagccg tcacgcgagc gctgccacac
    14101 cgcctcgatg cgcggctcgg cgactgcttc ctcacggccg tattcgcggg tttcgatgcg
    14161 gcgcccgacg agcctgccgc cgcccgtgct gccgaagaac agctggctct ggccgccgcg
    14221 cttgtcgtcg aaggccagcc agatgcgatc gccgatggtg gcggaatcca gcggcgcgag
    14281 catcgaggcg tcgtagacca gcttgttgtc gcgcaccagc agcgctaagt acagccagcg
    14341 ctcgtgagtg gcggccagca gctgcagaga atcgccgcca ggcccggcaa ctcgagtgag
    14401 gttgcgcgaa tcggtatccc attcatccgc gcgcccgtcg atgagtggcg cgccggacaa
    14461 cacgatgggc gtgatgtcac gcgcgttgcc atcgctcacc gccggcagtg aatcgtcgcg
    14521 gaacagcagt tcctgcctgc ccttgagcga cccggcgatc gtggtggtga cggccagcag
    14581 ggactgcttt tcgctttcgc gcagcacgtt ttccatctcg cgcgcgtact ggcagccggc
    14641 ccatggcagc gcgagagtgg tgagcgccag cagcagtagt ttggtccgca gccggaaccc
    14701 ggaaatcggt ccgatccgga tcaggtcgga aatgcgtccc acgatctgac tactcgaccc
    14761 agcggtagcc catgccgtac acggtgtgca cggcatcgaa tttctcgtcg accacctgga
    14821 atttgcggcg gatgcgcttg atgtgcgagg tgatggtgtt gtcgtcgagc accacgttgg
    14881 cggcgtccat gagctgctgg cggtttttca cgtgccccgg atgtttggtg agcgcgtaga
    14941 cgatccagaa ttccgtcaac gacagcagca cgacctggcc gttccattcc accgacatgc
    15001 gctcgctgtc gagcgagagc gcgccgcggc ggatgacctg cttttcctct gtggggcggc
    15061 gcagcgcgtc gacacggcga aataacgcat tcacccgggc gatgagatgc gcgagcgaca
    15121 ggtccttggt caggaagtcg tcggcgccga gccgcaggcc cgacaccgca tcgatttcgc
    15181 tgtcgcgggc agtcaggaaa atgatcggca gctcgggtga aagcccgcgc agttcgcggc
    15241 acaactcgaa accgccttcg ggctcgtctt ccagggaaat atcgatgacc gcgaggtccg
    15301 gcaggcgcgc cgcgaaagcc gccatggctt cacgccggtt tggataaccg cgtacggcat
    15361 atccctcacg cgagaaagcc gcggcgtagt tgtcgcggat ggcacgttcg tcttcgacga
    15421 tggcaatggt ctttttcaca ggacggcaag tgtaagcgac cggaatgggt gtcggtggtg
    15481 cggccacatt tggtcacttt cggtcggtgg aacgccatca aggcatcacg gatcggccgg
    15541 ctggccggcg cgaaatgtcg ctcaggccca aaacaaagga tgccgtcatg accgaccagc
    15601 ccaacgcatt cgaccggaca tatgctgccg aacagttcgc gacccagcag ggtgaacgct
    15661 ggcgccgctt cctggaacgc gtcggcacac gcagcaacga cttcgccgcg cggcttgctc
    15721 gtgcgcgttt cgtctacctg catcgccgcg aaacggccac gcactggttg ccgctgtgag
    15781 cgctgtgccc gggcatttca tgaacgagaa gccggaaacc cgcgggcccg tggcggagga
    15841 gaacgattcc gaggcatcgt tctggcactg gttcgcgctt ggcgcgctgc tgttcgcgtg
    15901 gattgccgag gcgacgatgt gtgccgaacg cggattctga agatgatttt cagtctggca
    15961 tcggtgcgag acccaggcgg gtcactcgcg aacgctgggc cagtgccagc acctgcgcca
    16021 cgacttcgta gcgcgtgcgt ctctccggta cgacattgat gcgtggctcg ttcgcgctgc
    16081 cggcgacagc cgcgaaacgt tcgccgagct gctcgagcga cgtcacttcc gcgccgttcc
    16141 agtagattgc gccgtcgaag gcgatatcca gccggataga tggctgtggc gccatggccg
    16201 ggccctgtgg caaattgagt ttcacggcat gtgttgccac cggtagcgtg atgatcagga
    16261 tcaccagcag aaccagcatc acgtcgatca gcggagtggc gttgagctgc ggctcggcga
    16321 ccgaggaatg acctggcatg tttcacctcc tgtatggcct aacgcgacag cgcactcgac
    16381 ggggttgcgg gcagtcatga gagccgaacc caggtacaag gcgctgccgc tcgaggccgc
    16441 cacgctgctc agcgaaggca acgtgatcga agccatcaag gtggtccgca agaccgaggg
    16501 tgcagggcgc agggaggcca aggcacgcgt cgatgcgcac atcgcccagg atccgatcct
    16561 gcaagtgcag ctcgaaacgc agcagcgcgc gagacgccgc aaattcttct tctggtttgt
    16621 tgtcgtggat ctcgcgatca ccgccgggat catttactgg cttttctaca gaggttccgc
    16681 ctgatgttgc tgatcggcat gttcgattca cctttcgtgc gccgtgttgc cgtttccatg
    16741 aagttgttag acattccgtt cgaacacggc aattggtcgg tgggcaagga cttcgaccgg
    16801 atccgcaact acaacccgct cgggcgcgta cccaccctgg tcaccgacac cggcgaatcc
    16861 ctcatggagt cgggcgcaat cctcgattac ctcgatgaac tggcgggacc tgagcgcgcg
    16921 ttgctgccgc gttccggcgc cgaacggcgc aaggcgctgc acctcatggc catggcgacg
    16981 ggcgcagcgg aaaaaggcct gctgcagatc tacgaacgga tctttcgccc ggagaccaag
    17041 cgccatcaac cctgggtcga tcgttgccgg ttgcaaatga gctcttcgct ggcggccatc
    17101 gatcgctacc ttggcgagcg aggcgtagcg cagtggctgg taggcaagcg gatgacgcag
    17161 gccgacatca ccgcggtttg tgcgttcacg ttcctgaacg acgcgctgcg ggtggccgac
    17221 gatgccgtca tgttccagtc gctcgccacg ctggcctctc gctgcgaatc catgcccgtg
    17281 ttccaggaaa cccgagtccc cttccacgct ccaaatagtt gatgaacgcc gccgcttcgg
    17341 cggcgttttc cgacgtggtc gtcagccctt aaaaagcact tatgcccgtg agctcacgcc
    17401 cgatcgtgag ctgatgaatg gtttcggtgc cttcgtaggt gatgacactc tcgagattca
    17461 gcgcgtggcg aatcggcaca tattcagcgc tgatccccga gccgccgagc atgtcacggc
    17521 agtcacgcgc gatgtcgagc gcgatgcggc agttgttcca tttggcgagc gacacctgtg
    17581 ccggctgcat cttgccctgg tccttgagcc ggccgagctg atgactgagc agctgcgcgg
    17641 tcgtgatctt tcgggccatc tccgcgagcc tgagctggat ggcctgcgtg gccgccaggg
    17701 gcttgccgaa catcacgcgc gtcttcgtgt agtcgagcag ctgtgcgagg caggcctgtg
    17761 ccgcgccgat gacaccccag gtaatgccgt agcgcgcctg cgtgaggcag gacaggggtc
    17821 ccttgagtcc ctttacaccc ggcaacaggt ttgactcggg taccaccacg ttgtcgaaga
    17881 acagtgaaga agtgaccgag gcacgcaggg aaaacttgcg ttcgatttcc ggcgccgcga
    17941 agcccttcat gcctttttcg atgatgaacc cgcgaatgcc gtcgtccgtc attgcccagc
    18001 agatgcagag gtcggcgatg gcgccgttgg tgatccacat cttcgagcca ttgatgaccc
    18061 agtcgccgcc tttctttttc gcgtgcgtct tcatgttcga agggtccgac ccgccgtgcg
    18121 gctcggtgag gccaaagcag ccgattttcc tgccgcgcgc catgtcgggc agccacttct
    18181 gcttctgttc atcgctgccg aaggcaaaga tcgggtacat gcacagcgag ctctgcaccg
    18241 agacgaaact gcggatgccc gaatccccgc gctcgagttc ctggcagatg agcccgtaac
    18301 tcacggcgtt caggccggcg cagtcgtaac cctgaatgga cgagcctaac aaccccagct
    18361 cggccagctc cgggatgagt tccttcggaa agtggtgctt ctcgaagcac tcctggatga
    18421 ccggcaggac tttctcgtcg acgaagcggc cgaccgagtc ctgcaccatg cgttcatctt
    18481 cggtcagcag accgcggatg tcatagagat cgaggggatt caacatcccg cgatgatacg
    18541 gtgccgggtg gggagtgggg agagcggctc tccccactcc ccacccggca cctgctagta
    18601 gcgcctgcta gtagccgata ccaagtcgac gatacaggtg gccgaggagc cagagcgggc
    18661 cgatcagcag gaactgcaga tccttgaaaa atgaaggccg cttgccttcg atggcgtggc
    18721 cgatgaactg gccgacccag ccgatggcaa aaatgaccag gcatgtgagc cacagtgg
//
LOCUS       DASQJS010000292         4858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3327182, whole genome shotgun sequence.
ACCESSION   DASQJS010000292 DASQJS010000000
VERSION     DASQJS010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3327182"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VEW08_11740"
     CDS             <1..971
                     /locus_tag="VEW08_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HYJ41456.1"
                     /translation="NLDLYACVRPIRYFPGVSTPMADASLTDMVVFRENTEDIYAGIE
                     WPTGSEEVHKLIHFLQSELKVTGIRFPASSGLGIKPVSKEGSQRLVRKAIQYAIENDR
                     VSVTLVHKGNIMKYTEGAFMQWGYLLAREEYGAKPIGAGPWLSFKSPVNGKEIVIKDV
                     IADNFLQQVLLRPEDYDVIATLNLNGDYISDALAAQVGGIGIAPGGNIGDGLAVFEAT
                     HGTAPGFAGKDRVNPGSLILSAEMMLRYIGWKEAADLIIRGVADTIAAKEMTYDLARL
                     REAIRATKRDIAARKNVEEDLQRLIPGATLLTCSGFGDAIIKRMRN"
     gene            1012..1659
                     /locus_tag="VEW08_11745"
     CDS             1012..1659
                     /locus_tag="VEW08_11745"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HYJ41457.1"
                     /translation="MTVTFRSDCVLCPRLARFLSVTLHENPDYHCRPVAPFGEAAPKL
                     LIVGLAPGMHGANRTGRPFTGDYAGILLYESLHAIGLSNSPKSVDRKDGLKLHGVLIT
                     NAVKCLPPENKPLPVEIKTCNQYLAAELAKLSSVTTVLALGTVAHDAVLMASGLKRSA
                     AKFGHGNEHELPDNRRMIDSYHCSRYNTQTRRLTTEMFQTVLRRAATHAGLSLAA"
     gene            1656..3485
                     /gene="uvrC"
                     /locus_tag="VEW08_11750"
     CDS             1656..3485
                     /gene="uvrC"
                     /locus_tag="VEW08_11750"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HYJ41458.1"
                     /translation="MSEHVDRFDPREYIPNLPRRPGVYRMYSESGELLYVGKARSLRD
                     RVGNYFLASNVDPKVQALIGHIARIEVTLTNSETEALLLEYNLIKEHKPRYNIVLRDD
                     KSFPYIYLPAGHEYPRLVFYRGSRNLPGRYFGPFPGAGAVKDTLQSVQKIFRIRNCRD
                     TFFENRSRPCLQHQIGRCSAPCVKLISREDYARDIAAAVKVLEGRNDEVYSELARTME
                     QAAESLQYERAAALRDQLAALKQVQAQQIVTAEGDRDIDVFALAGEPGEFAISVMIIR
                     GGRNLGSTCYFPKGSLAEPNEALASFLMQYYAETPPPAEVLVNLELAEADALIAALGE
                     RAGTALKLWKPSRALPARWMAMTVENASQSLRMRRARRADIGEMLVDLARLLQLPRTP
                     ERLECFDISHTAGEGTVASCVVYGPEGPMKKEYRRFNITGVTPGDDYGALRQALKRRY
                     ERVKAGEVPAPDVLLIDGGLGQINEVYAELEQLGFADLKLVGVAKGADRKPGQERLFV
                     HGDAGAIIPGPDSQALRVIQRIRDEAHRFAITGHRKRRARRHNESVLETVPGLGPAKR
                     RALLRHFGGMQGLLRAGVTDFEAVSGIGTTLARTLYDHLHPGA"
     gene            3498..4142
                     /locus_tag="VEW08_11755"
     CDS             3498..4142
                     /locus_tag="VEW08_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYJ41459.1"
                     /translation="MIWNLPNTLTWMRIFAIPLVIVLFYLPFTWADPAAGLLFAAAGI
                     TDSLDGYFARRMGQTSRLGAFLDPVADKLIVAVALVLLVSKEMPMIIVPGLEGLDGAR
                     ATLRAETVRVILVLCSVVIIGREIAISALREWMAEIGKRGKVKVSQLAKYKTILQIVG
                     LSCMLFRWNLPIFPGVSLPIFEIGLILTVIAAFLTLLSAISYLRAAWPDLRGSE"
     gene            4115..4705
                     /locus_tag="VEW08_11760"
     CDS             4115..4705
                     /locus_tag="VEW08_11760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HYJ41460.1"
                     /translation="MAGPAGFGMIRSVCVFCGSAPGARPSYSRAAEEMGAALANAGLT
                     LVYGGGKLGLMGIVADAVLKAGGRVVGVIPRMLIERECAHPSLTTQHVVTTMHERKTL
                     MADMSDAFVGLPGGMGTFDELVEIVTWAQLGLHAKPVILANIEDYYAPMYAMLDHAVQ
                     EKFVTPESRARWRNADSIESVMRILAKEGVTGEGSP"
     gene            4748..4823
                     /locus_tag="VEW08_11765"
     tRNA            4748..4823
                     /locus_tag="VEW08_11765"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4781..4783,aa:Gly,seq:gcc)
BASE COUNT          897 a         1545 c         1546 g          870 t
ORIGIN      
        1 agaacctcga cctgtacgcc tgcgtgcggc ccatccgcta tttccccggc gtatcgacgc
       61 ccatggctga cgcctcgctg accgacatgg tcgtgttccg cgaaaacacc gaagacatct
      121 acgccggcat cgagtggccg acgggttcgg aagaagtgca caagctcatt cacttcctgc
      181 agagcgagct caaggtcacc ggcatccgtt tcccggcgag ttcgggcctg ggtatcaaac
      241 ccgtgtcgaa ggaaggcagc caacgcctgg tgcgcaaggc catccagtac gccatcgaga
      301 acgaccgggt gtcggtcacg ctggtgcaca agggcaacat catgaagtac accgaaggtg
      361 ccttcatgca gtggggctat ctacttgcca gggaggaata tggcgccaag cccatcggcg
      421 ccggaccgtg gctgtcgttc aagagcccgg tcaacggcaa ggaaatcgtc atcaaggacg
      481 tcatcgccga caacttcctg cagcaggtgc tgctgcgtcc cgaagattac gacgtcattg
      541 ccacgctcaa cctcaacggc gactacatct ccgatgcgct ggccgcgcag gtgggcggaa
      601 tcggcattgc cccgggcggc aatatcggtg acggcctcgc cgtgttcgaa gccacgcacg
      661 gcaccgcgcc cggatttgcc ggcaaggatc gtgtgaatcc cggctcgttg atactttccg
      721 cggagatgat gttgcgttac atcggctgga aagaagccgc ggatctgatc atccggggcg
      781 tggcggacac catcgccgcc aaggaaatga cctacgacct ggcccggctt cgagaagcca
      841 tccgcgccac caagcgcgac atcgcggcgc gcaagaacgt cgaggaagac ctgcagcgac
      901 tgatcccggg agcaacgctg ctgacctgct cgggattcgg cgacgccatc atcaagcgca
      961 tgcgtaattg atcccgcgcg gggggccgcg caggcgttat cctcggtgtc gatgactgtc
     1021 accttcagat ccgactgcgt gctgtgcccg cggctcgcac gcttcctcag cgtcaccctg
     1081 cacgaaaatc ccgactatca ctgcaggccg gtggcgccct tcggcgaagc ggcgcccaag
     1141 ctgctgatcg tgggcctggc tcccggcatg cacggtgcga accggaccgg ccgcccgttc
     1201 acgggggatt acgcggggat actcttatat gagtccctgc acgcgatcgg gttgtcgaat
     1261 tcccccaagt cggtggaccg gaaagacggt ctcaagctgc acggcgtcct catcaccaat
     1321 gccgtgaagt gcctgccgcc cgagaacaag ccattgcccg tggagatcaa gacctgcaac
     1381 cagtacctgg cggcggagct ggcgaagctg agttcggtca ccaccgttct ggccctgggc
     1441 acggtggcgc acgacgcggt gctcatggcc tcggggctca agcggtcagc cgcaaagttc
     1501 gggcacggca atgagcacga gttgccggac aaccgccgca tgatcgattc gtaccattgc
     1561 agccgctaca acacgcagac acggcgcctg accaccgaga tgttccagac ggtgctccgg
     1621 agagccgcga cgcacgccgg tttatcattg gcggcgtgag cgaacacgtc gaccgtttcg
     1681 atccccggga atacatcccg aatctgccgc gccgtccggg cgtgtatcgc atgtactcgg
     1741 aaagcggtga actgctgtac gtgggcaagg cgcgcagcct gcgcgatcgc gtcggcaact
     1801 atttcctcgc gagcaacgtc gacccgaagg ttcaggcgct gatcgggcac atcgcgcgca
     1861 tcgaagtcac gctgacgaat tccgagaccg aggcgctgct gctcgagtac aacctcatca
     1921 aggaacacaa gccgcgttac aacatcgtgt tgcgcgacga caagagtttt ccgtacatct
     1981 atctacccgc gggacacgag tatccgcgcc tggtgttcta ccgcggctcg cgcaatctgc
     2041 cgggcaggta cttcggtcca ttcccgggcg ccggcgccgt caaggacacg ctgcagagcg
     2101 tgcagaagat cttccgcatc cggaactgcc gcgacacctt tttcgagaac cgcagccggc
     2161 cctgcctgca acaccagatt ggccgttgct cggccccctg cgtgaagctc atttcgcgcg
     2221 aggactatgc gcgcgatatc gcggcggccg tcaaagttct cgagggccgc aacgacgagg
     2281 tgtacagcga gcttgcgcgc acgatggagc aggccgccga aagccttcag tacgaacgcg
     2341 cggcggcgct gcgcgatcag cttgccgcgc tcaagcaggt ccaggcacag cagatcgtga
     2401 ccgcggaggg tgaccgcgac atcgacgtgt ttgcgctcgc tggtgagccg ggcgagttcg
     2461 cgatctcggt catgatcatt cgtggcggcc gcaacctcgg ctccacctgt tacttcccca
     2521 aggggtcgct cgcagagccg aacgaagcac tggcctcgtt cctcatgcag tactacgcgg
     2581 aaactccgcc gcccgcggaa gtcctcgtga acctcgagct ggccgaagcg gacgcactga
     2641 tcgccgcgct cggggagcgc gccggaacgg cgctgaaact gtggaaaccg tcgcgtgcgc
     2701 tacccgcgcg ctggatggcg atgacggtgg agaacgcctc ccaatcgttg cgcatgcggc
     2761 gcgcacggcg cgcggacatc ggcgagatgc tcgtcgacct ggcgcggcta ctgcaattgc
     2821 cgcgcacacc ggaacgcctc gagtgtttcg acatcagtca cacggcgggt gagggcaccg
     2881 tggcctcgtg cgtggtttac ggtcccgaag ggccgatgaa aaaggaatac cgtcgcttca
     2941 acatcaccgg cgtgactccc ggtgacgact acggagctct tcgtcaggcg ttgaagcgcc
     3001 ggtatgaacg agtcaaagcc ggggaggtac ccgctccgga tgtcctgctg atcgatggcg
     3061 ggctcgggca gatcaacgag gtgtatgcgg aactcgaaca gctgggattt gccgatctca
     3121 agctggtggg agtggccaag ggcgcggatc gcaagcccgg ccaggaacgt ttgtttgtcc
     3181 acggcgatgc gggcgcgatc attccaggcc cggattcgca ggccttgcgc gtgatccagc
     3241 gaatccgcga cgaagcgcat cgcttcgcga tcaccgggca tcgcaagcgg cgcgcacgcc
     3301 gtcacaacga atccgtgctc gaaaccgtgc cgggactggg acccgccaag cggcgcgcgc
     3361 tgttgcggca cttcggcggc atgcagggac tgcttagggc cggtgtcacc gacttcgagg
     3421 cggtgagcgg aatcgggaca accctggcgc gcacccttta tgatcatctg catcctggag
     3481 cctgataaat tcctccgatg atctggaacc ttccaaacac gctgacgtgg atgcggatct
     3541 ttgcgatccc gttggtcatc gtgctgttct atctaccgtt cacgtgggct gatccggccg
     3601 cgggcctgct gttcgcagcg gccgggatca ccgattcgct cgacggctac ttcgcgcgcc
     3661 gcatggggca gacctcgcgc ctgggcgcgt ttctcgatcc ggtggccgac aagctcatcg
     3721 tcgcggtggc gctcgtgctt ctggtgagca aggaaatgcc gatgatcatc gtgcccggcc
     3781 tcgagggcct ggacggcgcg cgcgcgacgt tgcgcgcgga gaccgtgcgc gtgatcctcg
     3841 tgttgtgctc ggtggtcatc atcggccgcg aaatcgccat ctcggcgctg cgtgaatgga
     3901 tggccgagat cgggaagcgt ggcaaggtca aggtgagcca gctcgccaag tacaaaacca
     3961 tactgcagat cgtcggcctg tcctgcatgc ttttccgctg gaacctcccg atcttcccgg
     4021 gagtgtcgct gccgatcttc gagatcggcc tgatcctcac ggtcatcgcc gctttcctca
     4081 cgctgctgtc ggcgatttcg tacctgcgcg ccgcgtggcc ggacctgcgg ggttcggaat
     4141 gatccgctcg gtctgtgtct tctgtggctc cgcgccgggc gcccggccta gctactcgcg
     4201 cgccgcggaa gagatgggcg cggcgctggc gaacgccgga ctcacgctgg tctacggcgg
     4261 aggcaagctc gggctcatgg gtatcgtcgc cgatgccgtg ctcaaggccg gcggacgcgt
     4321 ggtgggcgtg atcccgcgca tgctcatcga gcgcgagtgc gcgcatccga gtctcacgac
     4381 gcaacacgtc gtcacgacga tgcatgaacg caagacgctc atggccgaca tgtcggatgc
     4441 cttcgtcggc ctgccgggcg gcatgggcac attcgacgaa ctcgtcgaaa tcgtcacctg
     4501 ggcacagctc ggattgcatg ccaagccggt gatccttgcc aatatcgagg actactacgc
     4561 gccgatgtac gccatgctcg atcacgcggt tcaggagaaa ttcgtgaccc ctgaaagtcg
     4621 cgcccgctgg cgcaatgcgg attccatcga gtcggttatg cgaatccttg caaaagaagg
     4681 ggttaccgga gagggaagtc cttgacttgc cgggcgacgg ccctagaatt tgcgccctct
     4741 ccaggaagcg ggaatagctc agttggtaga gcacgacctt gccaaggtcg gggtcgcgag
     4801 ttcgagtctc gtttcccgct ccaaatccgc aggtaccctc cgttcgggcc cgtggcta
//
LOCUS       DASQJS010000293        13288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3633083, whole genome shotgun sequence.
ACCESSION   DASQJS010000293 DASQJS010000000
VERSION     DASQJS010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13288
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3633083"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VEW08_11770"
     CDS             <1..511
                     /locus_tag="VEW08_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HYJ41461.1"
                     /translation="MDYAADKADIFVTATGNFHVITHDHMKKMKNNAIVCNIGHFDNE
                     IDCASLKQYKWENIKPQVDHIIFPDGKRIILLAEGRLVNLGCGTGHPSYVMSSSFANQ
                     TIAQIELFTQTAKYPLGVYVLPKHLDEKVARLQLKTLNATLSELTDTQAKYIGVSKAG
                     PFKTDHYRY"
     gene            523..1392
                     /gene="folD"
                     /locus_tag="VEW08_11775"
     CDS             523..1392
                     /gene="folD"
                     /locus_tag="VEW08_11775"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF010783.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HYJ41462.1"
                     /translation="MTAKILDGKTVAAEERARSAARAADFVSKYGRSPGLAVVKVGED
                     PASAVYVRNKRKACEECGIASFAHDLPATTSREELLALIKALNADDRVDGILLQLPLP
                     KGLDSTEIMDTIDPAKDVDGFHPTNTGLLAQKRPGLRPCTPWGVIRLAQAYQLDLTGL
                     RAVVVGASNIVGRPMALELLLARSTVTVCHTGTRDLRGEVERAELVVAAVGKAAYIPG
                     EWIRERALVFDVGINRGADGKLCGDVAFAAAAERAAWITPVPGGVGPMTIAMLLSNTV
                     DAALARAGARKAR"
     gene            1429..2286
                     /gene="fdhD"
                     /locus_tag="VEW08_11780"
     CDS             1429..2286
                     /gene="fdhD"
                     /locus_tag="VEW08_11780"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HYJ41463.1"
                     /translation="MRSPVNEVQIDALRDSGNVHRADAIAVEEPLEIRLLKDGAAAAH
                     DNGGTGRPVSITMRTPGNDPDLAVGFLYGEGLLREPRDVIDARHCGPSGNVVRVTVRA
                     DLPLDLDRLNRNFYTTSSCGVCGKASIEAVTASAGIRRVSSDLVVRESVLRVMPDTLR
                     SAQAGFAETGGMHAVGLFTGEGELAAIREDVGRHNAMDKLVGAELMNGSLPWSQRVLL
                     LSGRASFELIQKAMMAGAPVVAAIGAPSTLAVELAEAAGITLVAFLRSGGCNVYCHAA
                     RVRPVRGAS"
     gene            2283..2762
                     /locus_tag="VEW08_11785"
     CDS             2283..2762
                     /locus_tag="VEW08_11785"
                     /inference="COORDINATES: protein motif:HMM:NF004638.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HYJ41464.1"
                     /translation="MTPARSAIETCVAAAVAAVKDEPGPLLEILHAVQNELGFVPSEA
                     VPLIAEALNLSRAEVHGVVSFYHHFRERPAGQHTIQLCRAEACQAMNGNALAEFAKRR
                     LQLDFGQTRADGKVTLEAVYCLGNCACAPSAMIDGQLRGRLDSKALADCADGWGVPA"
     gene            2759..4363
                     /locus_tag="VEW08_11790"
     CDS             2759..4363
                     /locus_tag="VEW08_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HYJ41465.1"
                     /translation="MSARAMTTVYVPGDAGAVSLGADALAAELARECRRREWPVRIVR
                     NGSRGLYWLEPLIEVKTGAGRIAYGPVHAAEIPALLDAGLLDGGPHALRLGDIEDHDW
                     LKTQQRLTVARVGVVDPADIDDYIAHGGYVGLGTALAMPPQDIVRAVTESGLRGRGGA
                     AFPTGIKWQTVHDQSAAQKYIACNADEGDSGTFSDRMIMEGDPFALIEGMTIAGLAVG
                     ATRGYIYLRVEYPHAARALARALHTARERGYLGRDLRDSGRDFDISVRLGAGAYICGE
                     ETSMLESLEGRRGEVRVRPPLPAIKGLFGQPTVVNNVITLATVPVILARGAAFYRDFG
                     MGKSRGTIPLQLAGNVKRGGLVERAFGLTVNQLVYDYGGGTATGRPVRAVQIGGPLGA
                     YLPESQFDTPLDYEALAARDGLLGHGGVVVFDDSINMARMARYALEFCAIESCGKCTP
                     CRVGSTRGVELVDRILVSSNGSRTGDLALLDELCNTMANGSLCGLGGLTPYPVRSALK
                     HFREDFSGAHAPLSKAAEAAASMKLG"
     gene            4368..7205
                     /gene="fdhF"
                     /locus_tag="VEW08_11795"
     CDS             4368..7205
                     /gene="fdhF"
                     /locus_tag="VEW08_11795"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HYJ41466.1"
                     /translation="MYAKNYHDFGTPAASGAPVTLEIDGATVTVPAGTSIMRAAALAG
                     GDIPKLCATDTLKAFGSCRVCLVEIEGRKGFPASCTTEVAPGMKVRTASDAVNNLRRG
                     VLELYVSEHPMGSCPDSSRCELETLAAAHGIGESRFADASSPRDLHTPKPVDASNPYF
                     RFDNDLCIVCSRCVRACEDTQGTFALTIEGRGIDSRVSASQQQPFLESECVSCGACVE
                     ACPTGALMEKSVITAGLPQQAITTTCAYCGVGCSLVAEVTDGAVSRMVPNRDGRANQG
                     HACVKGRFAYGYATHADRVTRPMIRKKITDEWLPVSWDEAINYAASEFKRIQAAHGRD
                     SIGGITSSRCTNEETFLVQKLVRAAFGTNNVDTCARVCHSPTGYGLKHTLGESAGTQE
                     FVSVQHADVIMVIGASPTEAHPVFASQMKRRLRAGARLIVIDPRATAIVKSPHVEADY
                     HLRLLPGTNVAVINALAHVVVTEGLTKDQYIADRCEAASYEKWRKFVADPVNSPEAME
                     KVSGVPAELIRGAARLYAQGPNSAIYYGLGVTEHSQGTTMVMGIANLAMATGNLGREG
                     VGVSPLRGQNNVQGSCDMGSFPHEYSGYRHVSDDATRALFESMWKVQLGSEPGLRIPN
                     MFEAALDGSFLGLYIQGEDFAQSDPNTAHVAAALSAMECIVIQDIFHNETSKFAHVFL
                     PGSSFLEKDGTFTNAERRISRVRRIIEPLAGLADWEVTVRLSNALGYPMNYAHPSQIM
                     DEIAALTPTFTGVNYEKLDRLGSIQWPCNEKTPQGTPTMHVGSFVRGKGKFYVTAYVP
                     TNERTSSRYPLLLTTGRVLTQYNVGAQTRRTANMAWHSEDRLELHPHDAEARGIEHGD
                     WVGITSRAGDTVLRVEITDRVAPGVVYTTFHFPESGANLITTENSDWATNCPEYKVTA
                     VQVVKVAQPAAWQMRRQAQVPVPVPAEPLGR"
     gene            7206..7424
                     /locus_tag="VEW08_11800"
     CDS             7206..7424
                     /locus_tag="VEW08_11800"
                     /inference="COORDINATES: protein motif:HMM:NF022832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HYJ41467.1"
                     /translation="MNSERLVAMANDIAAFFDAEPDKAVAAEGVRFHMTRFWDPRMRR
                     EIIAHVQVGGAGLCPTAMTAVQMLAPKP"
     gene            7446..7829
                     /locus_tag="VEW08_11805"
     CDS             7446..7829
                     /locus_tag="VEW08_11805"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ41468.1"
                     /translation="MNDIATSFPYATFLDLKFAPLEVVDVPQVVGAVKDRWFNQTLCK
                     VNESVLRLGVMQGEYHWHKHDNDDELFFVLEGRFFVDLEDRSVELLPWQGFTVPKGVV
                     HRTRAPQRAVILMMETASIVPTGDA"
     gene            7910..8794
                     /locus_tag="VEW08_11810"
     CDS             7910..8794
                     /locus_tag="VEW08_11810"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HYJ41469.1"
                     /translation="MIESLAAKLAARKFVVTGELTPPKGTDLSKLFAAAELLRDSVDA
                     INVTESPRARMTMDPRAAARLLQDRGFETIVQVTSRDRNRIAVQSDLLGAAALGLRNF
                     VFMGGDSTTGGDHPEAKPVFDLTASGLLAAAEALRNGRDYAGHPLHGTPDLFIGATCN
                     PGADRFEAEIENTRRKIDAGAKMLQTQAVYHGDPLKRFIDAVKPDGVAILAGVIPLKS
                     EKSGPWLNTNLPGVYVPQDMIDAMEEAARAGVARERGVELAARVVREMQGICQGVHIM
                     AIGWEAGVPEILRSAGVR"
     gene            complement(8795..9124)
                     /locus_tag="VEW08_11815"
     CDS             complement(8795..9124)
                     /locus_tag="VEW08_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41470.1"
                     /translation="MSSPIMTSPTALTASQCRAARALIEWSQAQLSQSAAIDIQTIAD
                     FEKRFRAADETTRRRLRATLEAAGVVFISENGGGAGARLKFSREEVWEVNRWEDEGGV
                     TGEDDVL"
     gene            9257..10297
                     /locus_tag="VEW08_11820"
     CDS             9257..10297
                     /locus_tag="VEW08_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ41471.1"
                     /translation="MRFRKLGNTGLIVSEMCLGAMTFGSGEGMWATVAGVSQEGVTDL
                     IKTAFDRGINFIDTADFYSNGRSEVVTGNALRELDLPRDSYVLATKVLLRMGPGHNQI
                     GLSRAHIMQGVDASLKRLQVDHIDLYQIHGRDPFTPMEETLDALDDCVRAGKVRYLGL
                     CNLSAWEIMKSQWISDKKNLARFESLQMYYSVAGRDIEREIVPLAKDQNLAIMPWSPL
                     AGGLLSGKFSRENEKPDGARRATLDFPPVDRERAFNVIDVMRPIAADNLVSVAQVALA
                     WLLAQPHVTSVIIGAKNQEQLLDNIGATELELSPEQLKAISDASALPNEYPQWMLTRQ
                     SGDRLGQVNGNS"
     gene            10375..11829
                     /gene="xylB"
                     /locus_tag="VEW08_11825"
     CDS             10375..11829
                     /gene="xylB"
                     /locus_tag="VEW08_11825"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HYJ41472.1"
                     /translation="MYLGIDIGTSSVKAVIVDDKDTVVEQASAPLNVSRPHAGWSEQN
                     PADWWSATNAAVKALPADLRKAVKAVGLSGQMHGATLLDAADKPLRPAILWNDGRSES
                     QCATLEKAEPRAREITGNIAMPGFTAPKLVWVRENEPAVFKDTRCVLLPKGYVRLLMT
                     GEKVGEMSDAAGTLWLDVGKRRWSPEMLAATGLSESHMPRLVEGSDASGTLLKAVASD
                     WGMDQVPVAGGGGDNAAGAAGIGVIKPGDAFLSLGTSGVLFLVTPKFLPNPAKAVHAF
                     CHCLPGVWHQMSVMLSAASCVDWAVKLTGTKDAAELLSKVEARGSLDGPEIFLPYLSG
                     ERTPHNDPQARGVLFGLNHDSDAAAVGQAVLEGVAFAFADGLDGLIEAGATIDKISVI
                     GGGARSMWWGGVLAAALKRPLIYRDASEVGPALGAARLARLAKTKERAEDVCRPPPVR
                     VVIEPNARDIEQLAPKRRAFSQIYQDLRPRFRGV"
     gene            11832..13172
                     /gene="xylA"
                     /locus_tag="VEW08_11830"
     CDS             11832..13172
                     /gene="xylA"
                     /locus_tag="VEW08_11830"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HYJ41473.1"
                     /translation="MSSAQPLAKPASYFGSVSPIKFEGLKSDNPLAFRYYNKDQIVLG
                     KRMEDLLRPAVCYWHSFAWNGHDIFGQGTFDRPWNAGAMDHTAAWAKMDAAFDFFTTL
                     GTPFYCFHDVDAMPAAANIKEHVSHLAATIDRLEKKQAETGIKLLWGTANLFSHPRYM
                     GGASTNPDPDVWAFAATQVRHCMDATKRLGGANYVLWGGREGYDTLLNTSLKHELANF
                     GRFLQLVVEHKHKIGFKGTILIEPKPHEPTKHQYDFDTATVYGFLKKNGLENEVKVNI
                     EANHATLAGHSFDHEIAMADALGIFGSIDINRGDPQNGWDTDQFHNDPLDMTLAMYRI
                     LKAGGFTTGGFNFDAKVRRQSIDPADMFYGHVGGLDLLARTLLSAAALIENGAIDKFV
                     EQRYARWAGDAGQKIHAKGASLESISDAAVAANINPKPQSGRQEFLENLVNRFV"
BASE COUNT         2436 a         4263 c         4335 g         2254 t
ORIGIN      
        1 gatggactat gcggctgaca aggccgacat cttcgtcacc gccaccggca acttccatgt
       61 gatcacgcac gatcacatga agaagatgaa gaacaacgcc atcgtctgca acatcgggca
      121 cttcgacaat gaaatcgatt gcgcttcgct gaagcagtac aagtgggaaa acatcaagcc
      181 gcaggtcgat cacatcatct tccctgacgg caagcgcatc atcctgctgg ccgaagggcg
      241 tctcgtgaac ctgggctgcg gcacgggaca tccgagctac gtgatgagct cgagcttcgc
      301 gaaccagacc atcgcgcaga tcgagttgtt cacgcagacg gcgaagtacc cgctgggtgt
      361 gtatgtgttg ccgaagcatc tcgacgagaa ggtggcgcgc ctgcagctga agacactcaa
      421 cgccacgctc agcgagctga cggacacaca ggccaaatac attggcgttt caaaggccgg
      481 tccgttcaag accgaccact accgttactg atcgcctacc tgatgacggc aaagatcctc
      541 gacggtaaaa ccgtcgcagc agaagagcga gcgaggtcgg cggcgagagc cgccgacttc
      601 gtttccaagt acggccgctc gccgggactc gcggtggtga aggtgggtga agacccggcc
      661 tccgcggtct acgtgcgcaa taagcgcaag gcctgcgagg agtgtggcat tgcctccttc
      721 gcgcacgacc tgccggccac gacctcacgc gaagagctgc ttgccctcat caaggcattg
      781 aatgccgacg accgcgtcga cggcatcctt ctgcagctcc cgctcccgaa gggtctcgac
      841 agcacggaga tcatggacac catcgatccc gccaaggacg tcgacggctt tcacccgacc
      901 aacaccgggt tgttagccca gaagcggccg ggcctgcggc cctgcacgcc ctggggtgtg
      961 atccggctcg cgcaggccta ccagctcgat ctcaccggcc tgcgtgccgt ggtggtcggc
     1021 gcttcgaata tcgtcggccg tccgatggcg ctggagttgc tgctcgcccg ctcgaccgtc
     1081 accgtgtgcc acaccggcac tcgcgacctg cgcggcgaag tggaacgtgc ggagctcgtg
     1141 gtggcggctg tgggcaaggc ggcctacatc ccgggcgaat ggattcgcga acgcgcgctg
     1201 gtgttcgacg ttggtatcaa tcgcggcgcc gatgggaaac tgtgcggcga cgtggcgttc
     1261 gcggcggcgg ccgagcgggc ggcgtggatc acgcccgtgc ccggcggcgt ggggccgatg
     1321 accatcgcca tgctgctgtc caatacggtg gacgccgccc tcgcgcgggc gggcgcacgt
     1381 aaagcccgct gacgtcgcgc ccgcgagccc gggtgtagac tcgggtacat gaggagcccg
     1441 gtaaacgagg ttcaaatcga tgcgctgcgc gactccggca acgtgcatcg tgccgatgcc
     1501 attgcggttg aagagccgct ggaaatccgt ttgctcaaag acggcgctgc tgcggcgcac
     1561 gacaacgggg gcaccgggcg gcccgtctcc atcaccatgc gcacaccggg taacgatccc
     1621 gacctggccg tgggatttct ctatggcgaa gggctgctgc gagagccgcg tgatgtcatc
     1681 gacgcgcggc actgcgggcc ctcgggcaac gtggtccgag tgacggtgcg cgccgatctg
     1741 ccgctcgatc tcgaccggct gaatcgtaat ttttacacca cctcgagttg cggcgtttgc
     1801 ggcaaggcct ccatcgaggc cgtcaccgcg agcgcaggaa tccgccgcgt gagcagcgac
     1861 ctggtagtta gggaatcggt cctgcgcgtc atgcccgata ctctcaggtc ggcccaggcc
     1921 ggatttgctg aaaccggcgg catgcacgcc gtcgggttgt tcacgggcga gggcgagctc
     1981 gccgccatcc gcgaagacgt gggccgtcac aacgccatgg acaagctcgt gggcgccgaa
     2041 ttgatgaacg gatctttgcc ctggagccag cgcgtgttgc tgttgtccgg ccgcgcgagc
     2101 ttcgaactga tccagaaggc catgatggcg ggagcgcccg tggtcgccgc gatcggcgcg
     2161 ccttcgacac tcgcggttga gctggcggag gccgcgggta ttacgctggt cgcgtttctg
     2221 cgcagcggtg gctgcaacgt ctactgccat gcggcgcgcg tgcgaccggt gcggggcgcg
     2281 tcatgacacc cgcgcgcagc gcaatcgaaa cctgcgtggc ggcggccgtc gccgccgtga
     2341 aggacgagcc cgggccgctg ctcgagatcc tgcatgccgt acagaacgag ctcggctttg
     2401 tgccatccga agccgtgccg ctgatcgcgg aggcgctcaa cctgtcgcgt gccgaagttc
     2461 atggcgtcgt gagcttctat catcatttcc gcgagcggcc ggccgggcag cacaccatcc
     2521 agctctgccg cgccgaagcc tgccaggcga tgaacggcaa cgcactcgcg gaattcgcga
     2581 agcggcgcct gcaactcgac tttggacaga cgcgcgcgga tggcaaggtc acgctcgaag
     2641 ccgtgtactg ccttggcaac tgcgcctgcg cgccctcggc catgatcgac ggccagctcc
     2701 gcgggcggtt ggacagcaag gcgctcgcgg actgcgcgga cgggtggggc gtacccgcat
     2761 gagcgcgcgc gccatgacca ccgtgtacgt acccggcgat gccggcgccg tttcattggg
     2821 agcggatgcc ctggccgcag agctcgcgcg ggaatgccgg cggcgcgaat ggccggttcg
     2881 aatcgtgcgc aacggttccc gcggactgta ctggctcgag cctctcatcg aagtgaaaac
     2941 cggtgccggc cgcatcgcct atggtccggt gcatgcggcg gagattccgg cactgctgga
     3001 tgcgggtttg ctggacggcg ggccgcacgc gctgaggctc ggcgacatcg aggaccacga
     3061 ctggctcaag acacagcagc ggctcaccgt ggcgcgcgtc ggagtcgtgg atccggcaga
     3121 catcgacgac tacatcgccc acggcggtta cgtgggcctg ggaacggcat tggcgatgcc
     3181 gccgcaggac atcgtgcgcg cggtgaccga atccggacta cgaggccgcg gcggcgccgc
     3241 gtttcccacc ggcatcaagt ggcagaccgt tcacgatcag tcggcggcgc agaaatacat
     3301 cgcctgtaat gcggacgaag gcgattcggg cacgttctcg gatcgcatga tcatggaggg
     3361 cgatccgttc gcgctcatcg aaggcatgac catcgcgggt ctcgcggtgg gcgccacgcg
     3421 cgggtacatt tatcttcgcg tcgaatatcc gcacgccgcg cgggccctgg cgcgcgcgtt
     3481 gcacactgcg cgcgagcgcg gctatctcgg gcgcgacctg cgggacagcg gacgggactt
     3541 cgacatctcc gtgcgcctcg gtgctggcgc ctatatctgc ggcgaagaga catcgatgct
     3601 cgaaagcctc gaaggacgtc ggggcgaagt gcgggtgcgg ccgccgcttc cggccatcaa
     3661 ggggttgttt ggccagccca cggtcgtcaa caacgtgatc acgcttgcaa ccgtgcccgt
     3721 gatcctggcg cgtggcgccg cgttctatcg cgatttcggg atgggcaagt ctcgcgggac
     3781 catccccctg cagctcgccg gcaatgtgaa gcgcggcgga ctggtggagc gcgccttcgg
     3841 cctcaccgtg aaccagctcg tctacgacta tggcggcgga acggcgacag gccggccggt
     3901 gcgcgcagtg cagatcggcg gccctctggg tgcctatcta ccagagagcc agttcgacac
     3961 tccgctcgat tacgaagcgc tggcggcgcg ggacggactg ctcgggcacg gcggggtggt
     4021 ggtattcgac gattcgataa acatggcacg catggcacgg tacgcactcg agttctgcgc
     4081 catcgaatcc tgcggcaaat gcacgccatg ccgcgtgggt tcgacgcgcg gcgtggaact
     4141 ggtcgatcgg attctggtca gttcaaatgg cagccgcacg ggtgatctcg cgctgctcga
     4201 cgagctctgc aataccatgg cgaacggctc gctgtgcggt ctcggcggct tgacgccgta
     4261 tcccgtacgc agcgccctga aacattttcg cgaagacttt tcaggggctc acgccccact
     4321 ttcaaaagcc gccgaagcgg cggcgtccat gaaattgggt taggactatg tacgccaaaa
     4381 attatcatga cttcgggacg cccgctgcca gcggcgcgcc cgtcacgctc gaaatcgatg
     4441 gcgcgacggt cacggtaccg gcgggcacgt ccatcatgcg cgctgcggca ctggcgggcg
     4501 gtgacatccc gaaactgtgc gccaccgaca cactcaaggc cttcgggtcc tgccgcgtat
     4561 gcctggttga gatcgagggc cgaaaaggct ttccggcctc gtgtaccacc gaggtggcgc
     4621 cgggcatgaa ggtgcgcacg gccagcgacg ccgtgaacaa cctgcgccgc ggcgtgctcg
     4681 agttgtacgt ttccgaacac cccatgggca gctgtcccga cagcagccgc tgcgagctcg
     4741 aaacactggc ggccgctcat ggcatcggcg agtcgcgctt cgcggacgca tcatcgccgc
     4801 gcgacctgca caccccgaag cccgtcgacg ccagcaatcc ctatttccga ttcgacaacg
     4861 atctctgcat cgtctgctcg cgctgcgttc gcgcctgcga agacacccag ggcacgttcg
     4921 cgctgacgat cgaagggcgg ggcatcgatt cgcgcgtgtc cgcctcgcag cagcagccgt
     4981 ttctcgaatc ggagtgcgtg tcctgcggcg cctgtgtcga ggcctgtccc acgggcgcat
     5041 tgatggagaa gtcggtcatc actgccgggc tgccgcagca ggcgatcacc accacttgcg
     5101 cgtactgcgg cgtgggatgt tcgctggtcg ccgaagtcac tgacggcgcc gtatcgagaa
     5161 tggtgccgaa ccgcgatggc cgcgcgaacc agggtcatgc ctgcgtaaag ggccgctttg
     5221 cgtacggata tgccacgcac gcggatcgcg tcacgcgacc gatgatccgc aagaagatca
     5281 ccgacgaatg gctcccagtc agttgggacg aggcgatcaa ctacgccgcc tcggagttca
     5341 agcgaatcca ggccgcgcat ggccgggatt ccatcggcgg tatcacgtct tcgcgttgca
     5401 cgaacgagga aaccttcctg gttcagaagc tggtgcgcgc ggcgtttggt accaacaacg
     5461 tcgacacctg cgcgcgtgtc tgccattcgc ccacgggtta tgggctcaag cacacgctcg
     5521 gtgaatccgc cggcacgcag gaattcgtgt ccgtgcagca cgccgacgta atcatggtga
     5581 tcggcgccag ccccacggag gcgcacccgg tattcgcctc gcagatgaag cggcgcctgc
     5641 gtgcgggagc gcggctcatc gtcatcgacc cgcgcgctac cgcgatcgtg aaatcgccgc
     5701 atgtcgaggc ggactatcac ctgcgcctgt tgccgggtac gaacgtggcc gtcatcaacg
     5761 cgctggcgca cgtggtggtg accgaaggtc tcacgaagga ccagtacatc gccgatcgtt
     5821 gcgaggcggc gtcgtacgag aagtggagga agttcgtcgc cgatcccgtg aattcgcccg
     5881 aggcgatgga gaaggtcagt ggtgtgccgg cggaattgat ccgcggcgcg gcgcggttgt
     5941 acgcacaggg cccgaacagc gccatctact acgggctcgg tgtcaccgag cacagccagg
     6001 gcaccaccat ggtgatgggc attgccaacc tcgccatggc caccggcaat ctcggccgcg
     6061 aaggcgtggg cgtgagtccg ctgcgcggcc agaacaacgt gcagggctcg tgcgacatgg
     6121 gttcattccc gcatgagtac agcggctacc ggcatgtgtc cgatgacgcg acacgcgcgt
     6181 tgttcgaatc gatgtggaag gtgcagctgg gttcggagcc ggggctgcgc atacccaaca
     6241 tgttcgaagc ggccctcgat ggcagcttcc tcgggctcta catccagggt gaggatttcg
     6301 cgcagtcgga tccgaatacc gcacacgtgg cggcggcgct gtcggccatg gaatgcatcg
     6361 tcatccaaga catctttcac aacgaaacct ccaaattcgc gcatgtcttt ctgccgggct
     6421 cgtcgttcct ggaaaaagac ggcacattca cgaatgccga acggcgcatt tcccgggtac
     6481 gaaggatcat cgagccattg gcggggctcg cggactggga agtcacggtg cgcctgtcga
     6541 atgcgctcgg ataccccatg aactacgcgc atccgtcgca gatcatggac gagatcgcgg
     6601 cgctcacgcc cacgttcacg ggcgtcaatt acgagaagtt agaccgcctg gggtcgatcc
     6661 agtggccctg caacgagaaa acgccgcaag gcacgccgac catgcacgtg ggttcattcg
     6721 tgcgcggcaa gggcaagttc tacgtcacgg cctatgtgcc cacgaacgag cgcacctcga
     6781 gccgttatcc gctgctgctg acgacgggcc gcgtgctcac gcagtacaac gtcggcgcgc
     6841 agacacggcg cacggccaac atggcatggc acagcgaaga ccggctggag ctgcatccgc
     6901 acgacgccga agcgcgcggc atcgaacacg gtgactgggt gggtatcacc agtcgcgcag
     6961 gcgataccgt gctgcgcgtg gagatcaccg accgcgtcgc gcccggcgtg gtgtacacca
     7021 cgttccactt ccccgagtct ggcgccaatc tcatcaccac cgagaattcc gactgggcca
     7081 ccaattgccc tgaatacaag gtgaccgcgg tgcaggtggt gaaggtcgcg cagccggccg
     7141 cgtggcaaat gcgccgtcag gcgcaggtac cagtgccggt gccggccgaa ccgctgggac
     7201 gctgaatgaa cagcgagcgg ctcgtcgcca tggccaacga catcgccgca tttttcgacg
     7261 cggagccaga caaggcggtt gccgcggaag gcgtgcgatt tcacatgacg cggttctggg
     7321 atccacgcat gcggagagaa atcattgcgc acgttcaagt tggcggagcg ggcctgtgcc
     7381 cgacagccat gacggcggtg cagatgctcg cgcccaaacc atgaccttgc catcgagacg
     7441 caaacatgaa cgacattgcg acgagttttc cgtacgccac ctttctcgac ctgaagttcg
     7501 cgccgctcga ggtggtcgac gtgccgcagg tggtgggtgc ggtcaaggac cgctggttca
     7561 accagacttt atgcaaggtc aacgaatcgg tgttgcggct cggcgtcatg cagggcgaat
     7621 atcactggca taagcacgac aatgacgacg agttgttttt cgtcctggaa gggcgctttt
     7681 tcgtcgacct cgaggatcgc agcgtcgagc tcctgccctg gcagggattc acggtcccga
     7741 agggcgtagt tcatcgaaca cgtgcgcccc agcgggcggt gatcctgatg atggaaaccg
     7801 cgtcgatcgt gcccaccggc gacgcctgaa cggcctaata gcccaaatca atcaaagaga
     7861 ccgccgatgc gctccccggg ccccgggctg cgtgtcagta ttgcgcccca tgattgaaag
     7921 cctcgcggcc aagctggccg cccgaaagtt cgtcgtgacc ggcgagctga ctccgcccaa
     7981 gggcaccgac ctgtcgaagc tgtttgccgc agccgagctg ctgcgagact ccgtcgacgc
     8041 catcaacgtc acggagtccc cgcgcgcgcg catgaccatg gatccgcgcg ccgcggctag
     8101 gctgctgcag gaccgcggct tcgagaccat cgtgcaggtc accagccgcg accgcaatcg
     8161 catcgccgtg cagtccgact tgttaggcgc tgcggcgctt ggcctgcgaa acttcgtatt
     8221 catgggcggg gattcgacga ccggcgggga tcaccccgaa gcgaaaccgg tattcgacct
     8281 caccgcgtcc ggcctgctgg cggcggccga ggcattgcgc aacggccgcg actacgccgg
     8341 ccatccgctg catggcacgc cggacctgtt catcggcgcc acctgcaatc cgggcgcgga
     8401 tcggttcgag gccgaaatcg agaacacccg gcgcaagatc gatgcgggcg cgaagatgtt
     8461 gcagacgcag gccgtgtatc acggcgatcc gctgaagcgc ttcatcgatg ccgtgaaacc
     8521 cgatggcgtc gcgatcctgg ccggcgtcat cccgctgaaa tccgaaaaga gcggcccgtg
     8581 gctgaatacg aatcttccgg gggtctacgt gccccaggac atgatcgatg cgatggaaga
     8641 ggcggccagg gcgggcgtcg cacgcgaacg cggtgtcgaa ctcgcggcgc gcgtcgtgcg
     8701 cgagatgcag ggcatctgcc agggtgtgca catcatggcc atcggctggg aggccggtgt
     8761 gcccgagatc ctgcgcagcg ccggagtacg ctgactaaag aacgtcgtcc tcgccggtga
     8821 cgccgccttc atcctcccag cgattcactt cccagacttc ttcgcggctg aacttcaggc
     8881 gcgcaccggc gccgccacca ttctcgctga tgaacaccac gccggccgct tcgagcgtcg
     8941 cgcgcaatcg gcggcgcgtg gtctcgtcgg ccgcacgaaa acgcttctcg aaatccgcga
     9001 tggtctggat gtcgatggcc gccgactgcg agagctgcgc ctgcgaccat tcgatgagcg
     9061 cacgcgccgc gcggcactgt gaggcggtga gggccgtggg agatgtcatg atcgggctgc
     9121 tcatggcggg atcgtagcaa acgcggggtt gctgacaatc caaattctct ggtggcccgc
     9181 aaaaggcata cttgcgtcgc acttacagcc ccgaacccct tcgcgcatac tcggccatac
     9241 accgatggag cagcacatgc gtttccggaa gctaggcaac actggtttga tcgtctcgga
     9301 aatgtgcctc ggggccatga ccttcggcag tggcgagggc atgtgggcca ccgtcgcggg
     9361 tgtgagccag gaaggcgtca ccgacctcat caagaccgcc tttgatcgcg gcatcaactt
     9421 catcgacacc gcggatttct attcgaacgg acgttcggaa gtggtcacgg gcaacgcgct
     9481 ccgggagctc gacctgccgc gcgattcata cgtgctcgcc accaaggttc tgctgcgcat
     9541 gggtcccggg cacaaccaga tcggcctctc gcgcgcgcac atcatgcagg gcgtggacgc
     9601 gagcctgaaa cgcctgcaag tggatcacat cgacctctac cagattcacg gccgcgatcc
     9661 gttcacgccg atggaagaga cgctcgacgc actggacgat tgcgtgcgcg ccggcaaggt
     9721 gcgctacctc ggcctgtgca acttgtcggc ctgggaaatc atgaaatcgc agtggatctc
     9781 ggacaagaag aacctcgcgc gtttcgagag cctgcagatg tattactcgg tggcgggccg
     9841 cgacatcgaa cgcgagatcg tgccgctggc gaaagaccag aacctggcga tcatgccctg
     9901 gagtccgttg gccggcgggc tgctgtcggg aaagttctcg cgcgagaacg agaagcccga
     9961 tggcgctcgt cgcgccacgc tcgacttccc gcccgtggat cgcgagcgcg cgttcaacgt
    10021 catcgacgtc atgcggccca ttgctgccga caatcttgtg tcggtcgcac aggtggcgct
    10081 ggcctggctg ctggcacagc cgcacgtcac ctcggtgatc atcggcgcca agaaccagga
    10141 gcagttgctc gacaatatcg gcgcaaccga actcgaactc agtccggagc agctcaaggc
    10201 catcagtgac gcctcggcgc tgccgaacga atatccgcag tggatgctca cgcgccagtc
    10261 gggcgaccgg ctgggacaag tgaatgggaa cagctgatca cggaatctgg aaacccagtg
    10321 ccctcggcgg caggtttctg gtagcgttac cgccgccaat tttcaggggg gcggatgtat
    10381 ctaggcatcg atatcggcac atccagtgtc aaggccgtca tcgtcgacga caaagacacg
    10441 gtggtcgaac aggcgtccgc gccgctcaac gtttcccgtc cgcatgccgg atggtctgaa
    10501 cagaatcccg ctgactggtg gagcgcgacc aacgccgccg tgaaggcgct gccggcggac
    10561 ctgcgcaagg ccgtgaaagc cgtgggcctg tcgggccaga tgcacggcgc cacgctgctc
    10621 gacgccgcgg acaagccgct gcggcccgcc atcctctgga acgacggtcg cagcgaaagc
    10681 cagtgcgcga cgctcgagaa ggccgagcca cgcgcccgtg aaatcaccgg caacatcgcc
    10741 atgcccgggt tcacggctcc caagctcgtc tgggtgcgcg agaacgaacc ggccgtcttc
    10801 aaggacacgc gctgcgtatt gctccccaag ggctatgtcc gactgctcat gacgggcgag
    10861 aaggtcggcg agatgtccga tgcggctggc acgttatggc tggatgtggg caaacgccgc
    10921 tggtcgcccg agatgctggc ggccactggc ctcagcgaat cgcatatgcc gcgcctcgtg
    10981 gaaggttccg atgcctcggg cacgctgctc aaagccgttg cctcggattg gggcatggat
    11041 caggtgccgg tggccggtgg tggcggcgac aacgcggccg gcgccgcggg catcggtgtc
    11101 atcaagccgg gcgatgcatt cctgtcgctg ggcacttcgg gcgtgctgtt cctggtcacg
    11161 ccgaagttcc tgccgaatcc cgcgaaggcc gtgcacgcgt tctgccactg cctgccgggc
    11221 gtctggcacc agatgagcgt gatgctgagt gccgcctcct gcgtcgactg ggccgtgaag
    11281 ctcaccggaa ccaaggacgc agccgagctg ctgtccaagg tggaggcgcg aggctcgctc
    11341 gatggccccg agatcttcct tccgtatctc tcgggcgaac gtacgcccca caacgatccc
    11401 caggctcgcg gcgtgctgtt cggcctgaac cacgattcgg acgccgctgc cgtcgggcag
    11461 gctgtgctcg agggcgtggc cttcgcgttt gcggacggcc tggacgggct catcgaagcc
    11521 ggcgccacca tcgacaagat ttcggtcatc ggcggcggag cgcgctccat gtggtggggc
    11581 ggtgtgctcg cggcggcgct caagcggccg ctcatttatc gcgatgcctc cgaagtgggc
    11641 cccgccctgg gcgccgcgcg gcttgcacgt ctagccaaga caaaggaacg ggcggaggat
    11701 gtctgccgtc cgccgccggt gcgtgtggtc atcgaaccca acgcccgcga tatcgagcaa
    11761 ctggcgccga agcgccgggc cttttcccag atttatcagg accttcgtcc aagattccga
    11821 ggagtttgac catgtcgagc gcgcaaccgt tagccaagcc cgcttcctat ttcggaagtg
    11881 tctcgcccat caagttcgaa ggcctgaagt ccgacaaccc gctggccttc cgctactaca
    11941 acaaggacca gatcgtcctc ggcaagcgca tggaagacct gctgcgcccc gcggtttgct
    12001 actggcactc gttcgcgtgg aatggtcacg acatcttcgg tcagggcacc ttcgatcgtc
    12061 cgtggaatgc gggcgcgatg gaccacaccg ccgcctgggc caagatggat gccgcgttcg
    12121 atttcttcac gacgctcggt accccgttct attgcttcca cgacgtggac gcgatgccgg
    12181 ctgccgcgaa catcaaggaa catgtctccc atctcgccgc gaccatcgac cgcctcgaga
    12241 agaagcaggc cgagacgggc atcaagctgc tgtggggcac ggcgaacctg ttctcgcatc
    12301 cgcgttacat gggcggcgct tcgacgaatc cggatccgga cgtgtgggcg ttcgcggcca
    12361 cgcaggtgcg tcattgcatg gacgccacca agcgtctcgg cggggctaac tacgtgctgt
    12421 ggggcggccg cgaaggctac gacacgctgc tcaacaccag cctcaagcat gagctcgcca
    12481 acttcggccg cttcctgcag ctcgtggtcg agcacaagca caagatcggc ttcaagggca
    12541 cgatcctcat cgagccgaag ccgcacgagc cgacgaagca tcagtacgac ttcgacacgg
    12601 cgaccgtgta cggcttcctc aagaagaacg gcctcgagaa cgaagtgaag gtgaacatcg
    12661 aagccaacca cgcgacgctc gcgggccaca gcttcgacca cgaaatcgcg atggccgatg
    12721 cgctcggcat cttcggctcc atcgacatca atcgcggcga tccgcagaac ggctgggaca
    12781 ccgaccagtt ccacaacgat ccgctcgaca tgacgctggc catgtatcgc atcctgaaag
    12841 ccggcggttt caccaccggt ggcttcaact tcgacgccaa ggttcgccgc cagagcatcg
    12901 acccggccga catgttctac ggccacgtcg gcgggctcga cctgctggcg cgcacgctgc
    12961 tgtcggcggc ggcgctcatc gagaacggcg ccatcgacaa gtttgtcgag cagcgttacg
    13021 ccaggtgggc cggcgacgcc ggacagaaga ttcacgccaa gggcgcgtcg ctcgagtcca
    13081 tctcggatgc ggcggtggcg gcaaacatca atccgaagcc gcagtcgggc cgccaggaat
    13141 ttctcgaaaa tctggtcaac cggttcgtct gaacgtgagc tcgtcgcaca cgatgtcgcg
    13201 cggtcgcagg tcggcagcgt tgctggtgct tgcggcagcc gcctcgtggg cggccgtcgc
    13261 gacgccggcg aaactcacaa aaacgctg
//
LOCUS       DASQJS010000294         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1749551, whole genome shotgun sequence.
ACCESSION   DASQJS010000294 DASQJS010000000
VERSION     DASQJS010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749551"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VEW08_11835"
     CDS             complement(<1..1317)
                     /locus_tag="VEW08_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41474.1"
                     /translation="MEPVNGARRWAVALMTASGFAALGYQIVWTQQSALWLGHETAAV
                     LAVVGAFFGGIAAGALAFSARIEASARPVRWYAACEAIIGLWSFALIFLMPIVSNLLL
                     DLIGAQPTSLRHGSVVFGATFLLLLPATAAMGATLPAMERIVGGLRADGAPIAILYAS
                     NTFGAVLGVLAAAFLLVPRFGLALTTMACVIANLFCALTALKVFTQRREAASPETMPE
                     GSSHLRVLAVTGLLGIAYEVLVVRVLSQVAENTVYTFAILLAVYLIGTTLGAAGYSRW
                     AKSAAEHERTRDRLLAALAAACLFGIACLAFAADGKIWLLQVLGTGMMPALIAEATLA
                     IAAFLLPTVLMGSLFSHLATTARADGVRFGRMIGVNTLGAALAPILAGSMLLSGIGTT
                     FSLLLMAAGYLLLTSRKAWTTPFQLTAAAALVALALWAPSLAFVQLP"
     gene            complement(1322..1945)
                     /locus_tag="VEW08_11840"
     CDS             complement(1322..1945)
                     /locus_tag="VEW08_11840"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HYJ41475.1"
                     /translation="MQEIIGLFPTPFMRAPGVLGSGLVNGLVEHFSKSVDLPNSSSPN
                     LVHTALLRPADSPLLVEVAALVNPLLPEFGALMFGERLGWSLKEMWVNVLYTGGRQAM
                     HNHANSFISGVVYLTRTHADARTVFMKSPGGTEFTFKNDHAGVTTGAYNADKWVSPPP
                     EPGDVVLFPSYLMHAVPLNPGERRISLAFNAIPTHLDSWGYRVSFNG"
BASE COUNT          414 a          636 c          657 g          299 t
ORIGIN      
        1 gggtagttgg acaaacgcca gggacggcgc ccacaacgcc agtgccacca gcgctgctgc
       61 ggccgtgagc tggaacggag tcgtccatgc cttccgggaa gtcagcagga gatatccggc
      121 ggccatcagc aacaacgaaa acgtggtgcc gatgccggat agcaacatcg agccggccaa
      181 tatcggcgcg agcgccgcgc ccagcgtgtt cacgccgatc atgcgcccga agcgcacccc
      241 gtcggcgcgc gcggtagtgg ccaggtggct gaacagcgat cccatcagga cggtgggcag
      301 aaggaacgcg gcgatcgcga gggtggcttc ggcgatgagc gctggcatca ttccggtccc
      361 taacacctgc agcaaccaga tctttccatc cgcggcgaat gccaggcacg cgattccaaa
      421 caaacaggcc gccgcaagcg ctgcaagcaa acggtcgcgc gtgcgttcgt gctcagccgc
      481 gctcttcgcc cagcggctgt agccggcggc gccgagcgtg gtgccgatga gatagacggc
      541 cagcagaatc gcaaaggtgt agaccgtgtt ctcggccacc tggctcaaca cgcggaccac
      601 cagcacttca taggcaatgc cgagcagacc ggtgacggcg agcacgcgca agtggctaga
      661 accttccggc atcgtctccg gcgacgccgc ctcgcgccgc tgcgtgaata ctttgagcgc
      721 cgtcagcgcg cagaacaggt tcgcgatcac acacgccatt gtggtgagcg caaggccaaa
      781 ccggggtacg agcaggaacg cggccgcgag cacgccaagc acggcgccga acgtgttgct
      841 ggcgtacaga atcgcaatcg gcgcgccgtc agcgcgcaaa ccgccgacga tgcgttccat
      901 cgccggcaac gtggcgccca tggccgctgt tgcgggcagc aacagcagaa acgtggcgcc
      961 gaacaccacg gacccgtgcc gtagcgacgt gggctgcgcg ccgatgagat cgagcagcaa
     1021 gttcgacacg attggcatca ggaaaatgag agcgaagctc cagagtccga tgatggcttc
     1081 gcaggccgca taccagcgaa cgggccgcgc actggcctcg attcgtgcgc tgaaggcaag
     1141 cgcaccggca gcaattccgc cgaagaacgc gccaaccacg gccagcaccg cggccgtctc
     1201 atggcctaac cacagcgcgc tctgctgggt ccacacgatc tggtagccca gggcggcgaa
     1261 gccggatgcc gtcatcaggg cgacggccca gcgccgggca ccattcaccg gctccacgag
     1321 atcacccgtt gaaggaaacg cggtagcccc aggaatccag gtgcgtcgga atggcattga
     1381 acgccagcga gatgcgccgc tcaccggggt ttagcggcac cgcgtgcatc aggtagctgg
     1441 ggaacagcac cacgtctcca ggctcgggtg gcggactgac ccacttgtcc gcgttgtagg
     1501 cgccggtcgt aacaccggca tggtcgttct tgaaagtgaa ttccgtgccg cccggggatt
     1561 tcatgaagac ggtgcgcgcg tcggcgtgcg tgcgcgtgag atatacgacc ccggaaatga
     1621 agctgttcgc atgattgtgc atcgcctggc ggccgccggt gtagagcacg ttcacccaca
     1681 tttccttgag tgaccagccg agtcgttcgc cgaacatcag agcgccgaac tccggaagca
     1741 gcggattcac caacgccgcg acttccacga gcagcggtga gtcggccggc cgcagcagcg
     1801 cggtgtgcac gaggttcggc gacgagctgt tgggaaggtc cacgctcttg ctgaaatgtt
     1861 cgacgagccc attcaccagg ccgctgccga gtacgcctgg ggcacgcatg aaaggcgtgg
     1921 gaaagagccc gatgatttct tgcattgcga agcttagtga cagttgtgcg acagattcat
     1981 gacggtgaag gccgtcgatg cgtcgc
//
LOCUS       DASQJS010000295         4650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1750908, whole genome shotgun sequence.
ACCESSION   DASQJS010000295 DASQJS010000000
VERSION     DASQJS010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750908"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="VEW08_11845"
     CDS             complement(<1..1384)
                     /locus_tag="VEW08_11845"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYJ41476.1"
                     /translation="MSHFPRSILGAAIAAVLATPGIVWAQSADATLRGKAPASTAVTA
                     KNVATGATRRTQANAEGYYSLTGLAPGTYRVDAGPGTETTVTLSVASTATLDLTASSG
                     AVEAPDAPMQEITVEGRRLNEVRTSEVGNTVSQHQIETLPQITRNFLEFADTIPGVVF
                     EVDGSGRTKISSGAQNRNGVNLYIDGVGQKGYVRSGVSGQAGDTQGNPFPQLAIGEYK
                     VITSNYKAEYDQITSAAITALSRSGTNEFDGEAFGTYSADNFRASTPSELDSGRKAES
                     ESKEYGFAFGGPIIQDHLHFFVTYEGKRFVTPVSVLTAFAPPDVASQLPADALAQLGP
                     TSIDFEEDLIFAKLDWALSERDSMQFSAKIRDEVSSGDRTGLGRARSATVNTNNDDKR
                     FELSWKHSADRWLNELQLTYEDAFYIPLPINTDANGAAYTWFDGQDRTVLEADGPDPR
                     AGQNKGQKGWA"
     gene            complement(1591..3855)
                     /locus_tag="VEW08_11850"
     CDS             complement(1591..3855)
                     /locus_tag="VEW08_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HYJ41477.1"
                     /translation="MRVTAALIALVALLSSPCFADTPAADPRDAALLARMTLDEKVNQ
                     LMLLSKGTMTGPDSAGRPNKPAEDLAREGIGFQMSAFEWSAKDINRIQRIAVRESRLG
                     IPVVFATDIIHGYWTVFPVPLGLSATFDPADARVVAGISGLEGYSHGQRWTFAPMVDH
                     PADPRWGRVVETFGESPLVSSDYAAATVRGFHGSLAQLPGKPAPVDFGVASCLKHYLG
                     YGSVQGGKDYAYVDLTERSIREFHLPPFAAGIAAGAPSVMPAFTTGPGGVPMSANKRM
                     LQDVLRGELGFNGVLVSDYAGITEMRNHGTAADDLEAAVQALRDGTMTVDMEDGVYYA
                     QLARAVRAGRLQIAEVDREVLRALAFKRRLGLFEKPYVPENLEKRVRVSTEHRAAARE
                     VARKSIVLLKNDANLLPLTAKRILVTGPLADAREDLLGPWHARGEAKDVVSVLAGIRE
                     RAASGKGTAVVGYSEGAKIDGKGKAVDGDDQRIAAAVAEARNVDLIIAVVGERESQSG
                     EAKNRAHLDLPGSQQALVDALAATGKPLVVVLITGRALAVPELVEQSGALVHAFFPGI
                     EGGHALADVLFGDYNPGGKQPVTWPRSVGQLPIHHYDPPNGRPNIPERGDYKAHWLDE
                     SDEPLFAFGFGLSYTRFELSALELPKRIGHAETLRVRVRLTNRGSRAGDEVPQLYIRP
                     RVAPAVTGKRLQAYRRVQLAAGESRVIEFAVPAKSLAVLDAQDRWAPQSGLYEVTLGT
                     SAQGGLAGTFELTD"
     gene            4018..4233
                     /locus_tag="VEW08_11855"
     CDS             4018..4233
                     /locus_tag="VEW08_11855"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HYJ41478.1"
                     /translation="MANRYGKKAEAKVKRAMHERKAGTLRSGRSGKKVTSRKQAIAIG
                     LSEARRAGGKVPPPPKGGRRRTRAKKK"
     gene            complement(4250..>4650)
                     /locus_tag="VEW08_11860"
     CDS             complement(4250..>4650)
                     /locus_tag="VEW08_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41479.1"
                     /translation="IPAGGDALQLRGPLLVFAVGCNFVLGALMTLGVGLYAPCLILVS
                     LLGMNPLTAFPIMMGSCAFLMPVAGLRFIAAQRYDARAALGLALGGIPGVLIAAFIVK
                     QLPLLWLRWLVVIVVLYTAIAMLRAARARS"
BASE COUNT          819 a         1467 c         1562 g          802 t
ORIGIN      
        1 tggcccagcc cttctgaccc ttgttctggc ccgcacgagg gtcggggccg tccgcctcca
       61 ggacggtccg gtcctgcccg tcgaaccagg tgtatgccgc accgttggcg tcggtattga
      121 tgggcagggg aatgtagaac gcgtcttcgt aagtgagctg caattcgttg agccaccggt
      181 cggcgctgtg tttccacgac aactcgaatc gtttgtcgtc attgttcgtg ttcacggtcg
      241 cgctgcgtgc gcgacccagg ccggtgcgat cgccggagct gacctcgtca cggatttttg
      301 ccgagaactg catgctgtcg cgttcggaga gcgcccagtc aagtttcgcg aagatcaggt
      361 cttcctcgaa atcgatagac gtgggtccga gctgcgccag cgcatcggcg ggcagttgcg
      421 aggccacgtc gggcggtgcg aatgccgtca gcacgctcac cggagtcacg aagcgcttgc
      481 cttcgtaggt cacgaagaaa tgcaggtggt cctggatgat gggtccaccg aatgcaaaac
      541 cgtattcctt gctctcggac tcggccttgc gtcccgagtc gagttcggac ggtgtgctcg
      601 cgcggaagtt gtcggcggag taagtcccga acgcttcgcc gtcgaattca ttggtgcccg
      661 agcgcgagag cgcggtgatc gctgcactcg tgatctgatc gtattccgcc ttgtagttcg
      721 acgtgatgac cttgtattcg ccgatggcga gctgcgggaa cggattgccc tgcgtgtcgc
      781 cggcctggcc gctgacacca gatcgcacgt agcccttctg gcccacgcca tcgatgtaga
      841 gattcacgcc attcctgttc tgcgcgccgc tgctgatttt cgtgcgcccg ctgccgtcga
      901 cctcaaaaac gacgcccgga atggtgtcgg cgaactccag gaagttgcgg gtgatctgcg
      961 gcagggtctc gatctggtgc tgggagacgg tgttgccgac ctcggaagtg cgaacttcgt
     1021 tgaggcgccg gccctcgacg gtgatctcct gcatcggcgc atcgggtgcc tcgaccgcgc
     1081 cgctcgaggc cgtgagatcg agtgttgccg tagaagcaac actcaacgtc accgtcgtct
     1141 cggtgcccgg gccagcgtcg acacggtagg tgcccggggc aagaccggtc agggagtaat
     1201 agccttcagc gttcgcctgt gtgcggcgcg tcgcgcctgt ggcgacattc tttgcggtga
     1261 cggcggtgct cgccggcgcc ttgccgcgca gtgtggcgtc cgcgctctgc gcccagacga
     1321 tgccaggtgt tgcgaggacg gccgcgatcg ccgcgccaag aatagaacgg ggaaaatgac
     1381 tcatggaaat ccctctcgag ttcggtcgtt cgcatgaagc gttgtgaata gcccacatgc
     1441 ttgctatgcg ccgattctct gcttccgcgc gcgcagaatc tcgatggttc agggatcctc
     1501 gtgtaggact aacacgcgga ctgagtgcgg cgttgtcgga caccgcaact ttgttaggac
     1561 caaagtagcg gagcgcatcg cagcaatggg tcagtccgtc agttcaaacg tgccagcgag
     1621 tccgccttgc gcactcgtgc cgagcgtcac ttcgtacagg ccgctttgcg gcgcccatcg
     1681 atcctgcgcg tcgagcaccg cgagactctt cgcgggaact gcgaactcga tgacacgcga
     1741 ctcacccgcc gccaattgca cgcggcggta ggcctgcagt cgtttgccgg tcacggcggg
     1801 tgcgactcgc ggccggatgt agagctgcgg cacttcgtcg ccagcgcggc tgccgcggtt
     1861 cgtcagtcgt acgcgcaccc tcagtgtctc ggcgtggccg atgcgcttcg gaagctcgag
     1921 cgcgctgagc tcgaagcgcg tgtatgacag gccgaagccg aacgcgaaca agggttcgtc
     1981 ggattcgtcg agccagtgcg ccttgtagtc accgcgctcg gggatgttgg gccggccatt
     2041 cggcgggtcg tagtgatgaa tcggcaattg accgacgctg cgcggccacg tgaccggctg
     2101 cttgcccccc gggttgtagt cgccgaacag cacgtcagcg agggcatgcc caccttcgat
     2161 gccgggaaaa aatgcatgca cgagcgcacc ggattgttcg accagctccg ggacggcgag
     2221 ggcgcgcccg gtgatgagca cgacgaccaa gggcttgccg gtggcggcca gcgcatcgac
     2281 gagcgcctgc tggctgccgg ggagatcgag atgcgcacgg ttcttcgctt cgccgctctg
     2341 ggattcgcgc tcgcccacca ccgcgatgat gaggtcgacg ttgcgcgcct cagccacggc
     2401 agcggcaatg cgctgatcgt cgccatcgac ggccttgcct ttgccatcga tcttcgcacc
     2461 ctccgagtag ccgaccacgg cggtgccctt gcccgaagca gcgcgctcgc gaatgcctgc
     2521 gagcacgctc accacatcct tcgcctcgcc gcgcgcgtgc cacgggccga gcagatcttc
     2581 gcgcgcatcg gcgagtgggc ccgtcacgag aatgcgcttc gcagtcagcg gcagcagatt
     2641 cgcgtcgttc ttgagcagca cgatggattt gcgcgcgacc tcgcgcgcgg cggcgcgatg
     2701 ctccgtggac acgcgcacgc gcttttcgag attttcggga acgtagggct tctcgaacag
     2761 ccccagccgg cgtttgaagg ccagcgcgcg taacacttcg cggtcgacct cggctatctg
     2821 cagacgtccg gcccgtacgg cacgcgcgag ctgcgcgtag tagacgccgt cttccatgtc
     2881 gaccgtcatg gtgccgtcgc gcagcgcctg cacggcggct tcgagatcgt cggcggcagt
     2941 gccgtgattg cgcatctcgg tgatgccggc gtaatcggac accagcacgc cgttgaagcc
     3001 gagttcaccg cgcagcacgt cctggagcat gcgtttgttg gcggacatcg gcacaccacc
     3061 cgggccggtc gtgaacgccg gcatgaccga aggcgcgccg gccgcgatgc cggccgcgaa
     3121 cggcggcagg tgaaactcgc ggatgctgcg ctccgtgaga tcgacgtagg cgtagtcctt
     3181 gccgccctgg accgagccgt agccgaggta gtgcttgaga caactcgcga cgccgaaatc
     3241 gacgggtgcc ggctttcccg gcaactgcgc gagcgagcca tgaaatccgc gcaccgtcgc
     3301 ggccgcgtag tcgctgctca ccagcggcga ttcaccgaaa gtttcgacca cgcggcccca
     3361 gcgcggatcg gcgggatgat cgaccatggg cgcgaaagtc cagcgctggc catgtgaata
     3421 accttcgagg ccggagatgc cggcgacgac tcgcgcatcg gcgggatcga aagtcgcgct
     3481 caggccgagc ggcaccggga agaccgtcca gtaaccgtgg atgatgtcgg tggcgaacac
     3541 caccgggatg ccgagccgcg actccctgac cgcgatgcgc tgaatccggt tgatgtcttt
     3601 tgccgaccac tcgaaagccg acatctgaaa gccgatacct tcgcgcgcca gatcttccgc
     3661 gggcttgtta ggccggccgg ccgagtccgg gccggtcatc gtgcccttgc tgagcagcat
     3721 gagctggttg accttttcgt cgagcgtcat gcgcgcgagc aatgcggcat cgcgtgggtc
     3781 cgcagcaggc gtgtcggcga aacacgggct ggacagcagc gcgacaagtg cgatcagggc
     3841 agcggttacg cgcatggatt ggttctcttg atgagtgagg ccggattgta aggcgcttca
     3901 tggaaacgca tcgctcatcg cgtacgcgaa gcgtgggcga cagcctacag cgcgatggcg
     3961 gcggcggcgg caagttgccg catcaatcga ctatcttcaa ccgacggagg agcggtcatg
     4021 gccaatcgat atggcaagaa ggccgaggca aaagtgaaac gcgcgatgca cgaacgcaag
     4081 gcaggaacgc tgcgcagcgg gcgctccgga aagaaggtga cgagccgcaa acaggccatc
     4141 gcgatcggcc tgtccgaggc gcgccgcgcc ggcggcaagg tgccaccgcc accgaagggc
     4201 gggcgccgaa gaacgcgcgc gaagaagaag tagcgccggg ttgtgccagt cagctgcgcg
     4261 cgcgcgccgc gcgcagcatc gcgatggccg tgtacagcac cacgatcacc accagccagc
     4321 gcagccacaa cagcggcagc tgcttcacga tgaacgcggc gatgagcacg ccgggaattc
     4381 cgccgagcgc gaggcccagg gcggcgcgcg cgtcgtagcg ctgggccgcg atgaagcgca
     4441 ggcccgcgac cggcatgagg aacgcgcatg agcccatcat gatgggaaag gcggtgaggg
     4501 gattcattcc gagcaggctg accaggatga ggcagggggc gtacaacccg acaccgagcg
     4561 tcatcaaagc gccgagcacg aagttgcagc ccaccgcgaa cacgagaagc ggcccgcgaa
     4621 gctgcaatgc gtcaccaccc gccggaatcc
//
LOCUS       DASQJS010000296         3928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_185679, whole genome shotgun sequence.
ACCESSION   DASQJS010000296 DASQJS010000000
VERSION     DASQJS010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185679"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VEW08_11865"
     CDS             <1..784
                     /locus_tag="VEW08_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41480.1"
                     /translation="QEESTYVRNPPYFEGMTMTPRPVTDVTGARCLALLGDSVTTDHI
                     SPAGDIAKTSPAAKYLVSKGVQPIDFNSYGARRGNHEVMMRGTFANIRLRNLLAPGTE
                     GGVTVHVPTGEQMSIFDAAMRYKQEGTPLVILAGKEYGTGSSRDWAAKGTMLLGVRAV
                     IAESFERIHRSNLIGMGVLPLMFPAGKGAATLGLTGSEVFEITGLDNAEAKTVKVVAT
                     PPKGSPVTFEARVRIDTPKERDYYRHGGILQYVLRQLAAKAA"
     gene            complement(868..1815)
                     /locus_tag="VEW08_11870"
     CDS             complement(868..1815)
                     /locus_tag="VEW08_11870"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HYJ41481.1"
                     /translation="MKRRVAFLIFPDFQLLDAAGPISAFEIAGRYAPGTYELRVIAKQ
                     PGAVASTSGASINAVAMGLPRSIDTLIVVGGEGTRAMSKDERIVKFVAACGAKSRRVA
                     SVCSGAYILAATGLLDGRAATTHWTRSTDFAKKFPRVKLDADRIFVKSGKFWSSAGIT
                     AGIDLSLALIAEDLGEAVARKVAQQLVVYYRRPGGQSQFSTLLELERADGRFAALLDY
                     VRTNLRERLSVDELAAQACMSPRNFARLFQAETGTSPARAVMRLRAETARAQLESGAS
                     SVQAVAQGCGFGDPERMRRAFQQIFGVAPSGVRRSRGAG"
     gene            1913..2605
                     /locus_tag="VEW08_11875"
     CDS             1913..2605
                     /locus_tag="VEW08_11875"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HYJ41482.1"
                     /translation="MAVPFNVVFALYPRITQLDFTGPYEVLTRLPGARCVLASVEGGE
                     LEVDSVMRFSGLRRLDSIDECDLLCVPGGFGTVAAVEDQAYLAALRRLGAKARYITSV
                     CTGSLLLAAAGLLKGKRAACHWAWRDKLADFGVTQDPGRVVRDGIIFTGGGVTAGIDM
                     ALEVMAEIGGRDLAETVQLAIEYAPAPPFDSGRPELAREPVLRAARARLDSVRAERDA
                     AIGRAAAALGAA"
     gene            complement(2616..>3928)
                     /gene="creD"
                     /locus_tag="VEW08_11880"
     CDS             complement(2616..>3928)
                     /gene="creD"
                     /locus_tag="VEW08_11880"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HYJ41483.1"
                     /translation="IVLLLDPLAQVESLVGERVEMRQTAAMRVAESWGGAQTTAGVLL
                     AIPVETTRVVVDQTAAGRETQRTEVERNVLFVLPDTLKISASADPGFRAVGLYETPVY
                     TANVQISGEFVNRDFAQLLTQKEGREVKWGEARLLVLNSESRALRAVDDLEVAGERST
                     VAADAYTGSAGISTSVPVAALRENATVPFRLKLTLAGSSQLSFLPLARKAEIAIKSAW
                     PHPKFQGAPAPLDPQITDDGFTARWSVLEINRNFGQSWYDSEVRPGEPAEASFAQSSV
                     GVVFYEPVDIYQRNYRAVHYAVLLIVITFLTFFLWEHMSGIAIHGMQYLMVGLALALF
                     YLLLLALSEHMAFDVAYATSAGALVALITIYLTGVLRRVTLALGAGAGLATLYTMLYW
                     ILRSEDYSLLMGALLLFGVLAALMIATRRIDWSNVVRLQRDADA"
BASE COUNT          687 a         1341 c         1253 g          647 t
ORIGIN      
        1 gcaggaagaa tccacctacg tccgcaaccc gccgtacttc gaaggcatga ccatgacgcc
       61 gcgtcccgtc acggacgtca ccggcgcgcg ctgcctggcg ttgctgggtg actcggtgac
      121 gaccgaccac atctcgcccg cgggcgatat cgccaagacg agccccgccg cgaagtacct
      181 cgtttccaag ggcgtgcagc ccatcgactt caattcctac ggcgcacgcc gcggcaacca
      241 cgaagtgatg atgcgcggca cattcgccaa catccgcctg cgtaacctgc tcgccccggg
      301 caccgagggc ggcgtcaccg tgcacgtgcc caccggcgag cagatgtcca tcttcgacgc
      361 ggccatgcgc tacaagcagg agggcacacc gctcgtcatt ctcgccggca aggaatacgg
      421 caccggctcc tcgcgcgact gggccgcgaa gggcaccatg ttgttaggcg tgcgcgccgt
      481 catcgccgaa agtttcgagc gcattcatcg ctccaatctc atcggcatgg gtgtgctgcc
      541 gctcatgttc ccggccggca agggcgcggc cacgctcggc ctcacgggtt cggaggtctt
      601 cgagatcacc gggctcgaca atgccgaggc caagaccgtg aaggtcgtgg ccacgccgcc
      661 gaagggatca ccggtgacgt tcgaggcgcg cgtgcgcatc gacacaccga aggagcgcga
      721 ctactaccgt cacggtggca tcctgcagta cgtcttgcgg cagctcgccg ccaaggctgc
      781 gtaaccaggg cgtaggggtg ccgggtgagg agtggggagt cgtactcccc tgctccaccg
      841 cctgccaccg cgccgactcc ccactcctca cccggcacct cggctgcgcc gcacacccga
      901 cggcgccact ccgaatatct gctggaatgc gcgccgcatg cgctccggat cgccgaagcc
      961 gcagccttgc gccacggcct gcaccgacga cgcgccgctc tcgagctgcg cccgcgccgt
     1021 ctcggcacgc agccgcatca ccgccctggc cgggctggtg cccgtctcgg cctgaaacag
     1081 acgcgcaaaa tttcgcgggc tcatgcacgc ctgcgccgca agttcatcga ccgagagccg
     1141 ctcgcgcagg ttggtgcgca cgtagtcgag caacgctgcg aaacgtccat cggcgcgttc
     1201 cagctcgagc agggtcgaga actgcgactg tccacccggt cggcggtagt agacaacgag
     1261 ctgctgcgcc accttgcgcg ccacggcctc gccgaggtct tcggcaatga gcgccagcga
     1321 cagatcgatt cccgcggtga tgccggccga gctccagaat tttcccgact tcacgaagat
     1381 gcgatcggcg tccagcttca cgcgtggaaa cttcttcgca aagtcggtgc tgcgcgtcca
     1441 gtgggtggtg gcggcgcgtc catcgagcaa accggtggcg gcgagaatgt aggctcccga
     1501 acatacgctg gccacgcgtc gtgatttcgc gccacaggcg gcgacaaact tcacgatgcg
     1561 ctcatccttc gacatcgcac gtgtgccttc gccaccgacg acgatgagcg tgtcgatcga
     1621 cctgggcagc cccatcgcca cggcgttgat actggcgccg gaagtgctcg cgacggcgcc
     1681 tggctgcttc gcaatcacgc gcagctcata cgtccctggc gcatatcgcc cggcgatttc
     1741 gaacgcggag atcggcccgg cggcatcgag cagctggaag tccggaaaaa tgagaaaggc
     1801 aacgcggcgc ttcatcgctt catggcataa aacgagggaa caatggcatt ccggccagca
     1861 ggctagtgcg cgaggatggc gccgtcaaca ttttcgcctg caggagcaac ccatggccgt
     1921 cccattcaac gtcgttttcg cgctctaccc tcgcatcacc cagctcgatt tcaccgggcc
     1981 ctatgaagtg ctcactcgcc tgccgggcgc acgctgcgtg ctcgcgtcgg tggaaggtgg
     2041 cgaactcgag gtggattcgg tcatgcgctt ttccggacta cgccggctgg acagcatcga
     2101 cgaatgcgac ctgctctgcg tgccgggcgg cttcggcacg gtggcagccg ttgaagacca
     2161 ggcctatctc gcggcgctgc gccgcctggg cgcgaaggcg cgctacatca cctcggtgtg
     2221 cacgggctcc ctgctgctcg cggccgcagg actgctcaag ggaaagcgcg ccgcctgcca
     2281 ctgggcgtgg cgcgacaaac tggccgactt cggcgttacg caggaccccg gccgcgtcgt
     2341 gcgcgacggc atcatcttca cgggcggcgg cgtcaccgcg ggaatcgaca tggcgctcga
     2401 ggtgatggcg gaaatcggcg gccgtgacct cgccgaaact gttcaactcg ccatcgaata
     2461 tgcgccggct ccgcccttcg actccggtcg tccggagctg gcgcgcgagc cggtgctgcg
     2521 cgccgcgcgg gcgcgtctcg attcggttcg cgccgagcgc gacgcggcta tcgggcgggc
     2581 cgccgccgcg ctcggcgccg catgattcat tgcttctacg catccgcatc acgctgcagt
     2641 cgcactacgt tcgaccagtc gatacggcgc gtggcgatca tcagtgcggc gagcacgcca
     2701 aacagcaaca gcgctcccat gagcagcgag tagtcctcgg agcgcaggat ccagtacagc
     2761 atggtgtaga gagtcgcgag ccctgccccg gcgcccaacg ccagagtgac ccgccgcaac
     2821 acgccggtga ggtagatggt gatcaacgcc accaatgcgc ctgcagacgt cgcgtacgcg
     2881 acgtcgaacg ccatgtgttc ggacagtgcc agcagcaata gatagaacag cgcgagcgcc
     2941 agccccacca tgagatattg catgccgtga atggcaatgc cgctcatgtg ctcccagagg
     3001 aagaacgtga ggaatgtgat cacgatgagc agcaccgcgt agtgcaccgc gcggtagttg
     3061 cgctggtaga tgtcgacggg ttcgtagaac accacgccaa cgctgctttg ggcgaaagat
     3121 gcctccgcgg gctcgcccgg acgaacctcg ctgtcgtacc agctctgacc gaagttccgg
     3181 ttgatctcga gtaccgacca gcgggccgtg aatccgtcgt ccgtgatctg cgggtcgagt
     3241 ggcgcgggcg cgccctggaa cttcggatgc ggccatgcgg acttgatggc gatttccgcc
     3301 ttgcgcgcga gcggcaggaa actgagctgg ctgctgcccg ccagggtgag tttcagccga
     3361 aacggaaccg tggcgttctc gcgcagcgcc gccacgggta cggacgtgga aatgcccgcg
     3421 gatcccgtat aagcgtcggc cgccactgtc gacctttcgc cggccacctc gaggtcgtcc
     3481 acggcgcgca atgcgcgcga ttcggagttg agcacgagca ggcgcgcctc gccccatttc
     3541 acctcgcggc cctccttctg cgtcagtagt tgggcgaagt cgcggttcac gaactcgccg
     3601 gagatctgca cgttggcggt gtacaccggt gtctcgtaaa gtccgacggc gcgaaaaccc
     3661 gggtcggcgc tggcgctgat cttcagcgta tcgggcagca cgaaaaggac attgcgctcg
     3721 acctccgtgc gttgggtttc tcgccccgcc gcggtctggt cgacgaccac gcgcgtggtc
     3781 tccacgggaa tcgccagcaa aacgcctgcg gtggtctgcg cgccacccca ggattcggcc
     3841 acgcgcatcg cggcggtctg ccgcatttcg actcgttcgc ccacgaggct ttccacctgg
     3901 gcgagcggat cgagcaacaa cacgatca
//
LOCUS       DASQJS010000297         7923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3636564, whole genome shotgun sequence.
ACCESSION   DASQJS010000297 DASQJS010000000
VERSION     DASQJS010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3636564"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="VEW08_11885"
     CDS             <1..1704
                     /locus_tag="VEW08_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41484.1"
                     /translation="MLAANTPLPVPEDLMGAVETRIGKLPAGTVAMLEAAAVCGVEFR
                     AGAVADILGRTRQEIIEECDRLVQKQYWLRHSATVDLSDGTLDALYTFRHSIYRHVFY
                     ERMGVAPRVQLHRRVAQVLAAGAALGVAVAATELASHHERGREPAAALRAYSQAAQGA
                     LRSFVPLQAFEICEHARALLSQVPAGPERLMLELGIESPRGVAAAQVHGVGSAEPRAI
                     FERVRELCELLPQHPARALLLNGYGASLFSRGEYGKLQEFAERLELLEGPDRAPLLVM
                     TALFRAGAMAARGECRVATEWWKKAIEYCEAITDRSGFQAFIVDPETGIRANAVRTLF
                     ERGLIDQARAQSAKSVAMAEALGQPLAQSLARWRAGMLEVRLGNPDKVIEHANAIEKI
                     VARTSVVQGDGPSRYLRGWALAQLGDPQTGLAHIRDGLARHLRIGMISSSTEVMGYAA
                     EALVLAGDWQGANKELADAFARARDLDENFYVPILLMLQARVASGLGDEKAAYRWLRE
                     AVQVALAQEACGFELKASCALVEHPLSTQEDKIALLELLKTFTEGIDSKDLIRARLLV
                     N"
     gene            1788..1988
                     /locus_tag="VEW08_11890"
     CDS             1788..1988
                     /locus_tag="VEW08_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41485.1"
                     /translation="MNKHESNPKQSNWTGSRLASARWQELHNHLSRHPATAEYGWACR
                     SSLICKWRAAPVAVLQATSPVG"
     gene            1993..2190
                     /locus_tag="VEW08_11895"
     CDS             1993..2190
                     /locus_tag="VEW08_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41486.1"
                     /translation="MILAIHPSYPNARAYVLKLHRDARPAAGKVIGRLENVTTGEQFV
                     FGSAEELLAALARDVSSTRFE"
     gene            2199..2621
                     /locus_tag="VEW08_11900"
     CDS             2199..2621
                     /locus_tag="VEW08_11900"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HYJ41487.1"
                     /translation="MDSNLSAEASIVVTVQDTASRIALAPTDSFPEVFATSRMIALME
                     LAAARAMMPLLGEGQLSVGVSINVRHTAATLPGSHVRAVATRISTEAKLMYFRVEAFD
                     EAGLIGTGEHARAIVDTTRLISGAERRRKPISNTQGIQ"
     gene            2618..4033
                     /locus_tag="VEW08_11905"
     CDS             2618..4033
                     /locus_tag="VEW08_11905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HYJ41488.1"
                     /translation="MKPLIALLGVAALSACASLPGKAPAPALKPQDSFASTQMFAAPA
                     GEWPAAGWWKVYGDTQLNALIDEGLAGSPSIAVADARLRRARAAQDTSRGSLMPQVSA
                     SGSMTEQKQSYNYLSPAAMTPQGWNDYGRASLDFSWELDFWGKNRAALAAATSEAVAA
                     SADADQSRLALSSAIASSYAELARLYSALDTAIAARDLRTKTAELFGKRFDNGLETRG
                     GQRQADSRRAASEADVLSLEEQIALQKNRIAALMGAGPDRGLSIARPKVDFTRAFALP
                     STLSVDLIGRRPDLVAARLRAEAAAKRIHVTRAQFYPNVNLSAFIGVQSLGLDLLQEN
                     GSDIGSVGPAFTLPIFNGGRLRGQLRGAEAEYAEAVANYDRALVQALQEVADAAVSQR
                     ALGAQIGYIDAAVDAARESWRVQSNRYEGGLASYLDVLSAEDYLLGNLRTQADLRSRS
                     MTLDVALNRALGGGYQSSSNL"
     gene            4060..5184
                     /locus_tag="VEW08_11910"
     CDS             4060..5184
                     /locus_tag="VEW08_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016253239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HYJ41489.1"
                     /translation="MTTMNTEVLGKADQALEQAADQAAKAKRKRALLVLGAVVLTLGT
                     SYGTYSHFYASKFISTDNAYTAAETAQVTPSVGGIVREVRVTDTSYVHKGDVLVVLDD
                     IDARLALAQAEAELGRAQRRVRGYVANDESLTAQLAARAADEKRAKADLDRAVIDLQR
                     RENLAESGSVSGDELTKARNAYESARASLAQARSNYTAAIGSRNANAVLIENTTESTN
                     PEVALARARRDQAQVNLDRTVIRAPVDGVVAKRSVQVGQQIQAGSPVLAIVPLAEVHV
                     DANFKEGQLERVRIGQPVEITSDLYGSSKTYHGVVVGLSGGTGSTFAAIPAQNATGNW
                     IKVVQRVPVRIRLDTAELEANPLQVGLSMEATIDTRSNQG"
     gene            5212..6753
                     /locus_tag="VEW08_11915"
     CDS             5212..6753
                     /locus_tag="VEW08_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HYJ41490.1"
                     /translation="MTAFEKALSSDAPLKGAMLWLVAIVLAGANFIAVLDMTIANVSV
                     PNIAGSLGISSSQGTWVITCYAVAEAITVPLTGWLAARFGAVRVFTTAIGCFGLFSLF
                     CGLANSFAMLVVGRILQGVAGGPLIPLSQTLLLRIFPKEKAAAATALWAMTTLVAPIL
                     GPILGGYLCDNYAWPMIFLINVPLALAMAPFALRMLKRFETAIVRTPIDLIGLAIMVL
                     AVGALQLMLDLGKEHDWFESGLIISLGIVAAIGLVAFVIWEMGEKHPIVNLRVFRHRG
                     FTTAVIVIAAGFGAMFAANVLTPLWLQSYMGYTSSWAGYATAWTGVLAVFCAPAAGLL
                     STKVDPRKLVFFGLVWLGGVLAFRTGATTDMTFWQISGPLLLMGLGLPFFFVPVTTLA
                     LASVEEHETASAAGLMNFLRTLAGAVATSVVTTSWESKTQVMHSELAGVVDRTGEVAH
                     TLGASGFSPEATRQALDNMTQSQSVMLATNEIMAFVSLAFFLSAMLIWAAPRPTRKVA
                     LGAAH"
     gene            6773..7240
                     /locus_tag="VEW08_11920"
     CDS             6773..7240
                     /locus_tag="VEW08_11920"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HYJ41491.1"
                     /translation="MTQSLLTYRGTVHSWHCDHMGHMNVMWYVGKFDEATWYLAAMMG
                     MTAQYLKDAKRGMAAVDQRIAYRREALPGDVVTIRTAIIEVRQKSVRFVHEMRRGDLG
                     DLLATMVVTGVHIDGVGRRATAFDKQIHAKAEEMITPDAALWDAWPPEKAYLD"
     gene            complement(7316..>7923)
                     /locus_tag="VEW08_11925"
     CDS             complement(7316..>7923)
                     /locus_tag="VEW08_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ41492.1"
                     /translation="LVVPLTRRITIPNTGLSTNDFVVTQPVNASDGVLKGFELGFQYF
                     PELPGVFNGFGILGSYTSLDSSQNIPQTDSAGNIIGEETTDFFVVSDSSYNASLAYEH
                     GGFGGRLSYVWREKFLNNNEARIFANPIGIWRRPESSLDLQLNYDFNENFAISFDAVN
                     LTDELTQSYYHFADVGDPLHTNFGNVLNGRQFAIGVRWRAN"
BASE COUNT         1412 a         2458 c         2635 g         1418 t
ORIGIN      
        1 atgctcgcag cgaacacgcc gttgcccgtt cccgaagacc tcatgggtgc ggtggaaacc
       61 cgcatcggca aactaccggc gggaaccgtg gccatgcttg aagccgcggc ggtgtgcggt
      121 gtggagtttc gcgccggtgc ggtggccgac atactcggac gaacgcggca ggaaatcatc
      181 gaagagtgcg atcggctggt gcagaagcaa tactggcttc gccacagcgc caccgtggat
      241 ctgagcgacg gcacgctgga tgcgctctat acattccgcc acagcatcta tcggcacgtg
      301 ttctacgagc gcatgggcgt ggcgccgcgc gtgcagctcc accggcgtgt ggcacaggtg
      361 ctggctgcgg gtgcggcgct gggcgtagct gtcgctgcga ccgagctcgc ttctcatcat
      421 gagcggggca gggagccggc ggcggctttg cgcgcgtact cccaggcggc gcagggtgcg
      481 cttcgcagtt tcgtgccgct gcaggccttc gagatctgcg agcacgcgcg tgcgctgttg
      541 tcgcaggttc ctgccgggcc tgagcgcctg atgctcgagc tcggcatcga gagtccgcgc
      601 ggtgtcgctg ccgcacaggt acacggagtc ggctcggccg agccgcgcgc cattttcgag
      661 cgagtacgtg aactgtgtga gctcctgcca caacacccgg cacgggcgct gctgctcaac
      721 ggttatgggg catcgctctt ttcgcgaggc gagtacggca agttgcagga gttcgccgag
      781 cggctcgagt tgctcgaggg acccgatcgc gcgccgctgc tggtcatgac cgcgttgttt
      841 cgtgctggtg ccatggcggc gcggggcgag tgccgggtcg ccacggaatg gtggaagaag
      901 gccattgaat attgcgaagc catcaccgat cgaagcggct ttcaggcctt catcgtcgat
      961 ccggaaaccg gcatccgcgc caatgccgtg cgcacattgt tcgaacgagg gctcatcgac
     1021 caggcgcgcg ctcagtctgc gaagtcggtt gccatggcgg aggcgctcgg tcagccgctg
     1081 gcgcaatcgc tggcacggtg gcgcgccggc atgctcgaag tacggctcgg caacccggac
     1141 aaggtcatcg aacacgccaa tgcaatcgag aagatagtcg ccaggaccag cgtggtgcag
     1201 ggcgacggcc ccagccgcta cctgcgcggc tgggcgctgg cgcaactcgg cgatccccaa
     1261 acgggtctcg cgcacattcg cgatggactg gcccggcacc tgcgcatcgg catgatctcg
     1321 agcagcacag aggtgatggg ctacgccgcc gaggcgctcg tcctggcggg cgactggcaa
     1381 ggcgccaaca aagagctggc ggacgccttc gcccgtgcac gcgatctcga cgaaaacttt
     1441 tacgtgccga tcctgctcat gctgcaggcg cgcgtggcga gcgggctggg tgatgagaag
     1501 gcggcgtatc gctggctgcg ggaagccgtg caggtggcgc tcgcgcagga ggcttgtggc
     1561 ttcgaactga aggcgtcatg cgcgctcgtc gaacatccgc tcagtacgca agaggacaaa
     1621 atcgccttgt tggagttact gaagacattc acggaaggta ttgattctaa agatctaata
     1681 cgtgcccgtt tactggtcaa ctgatcccag accgaaacgc gaccgcagcg ttacgacttc
     1741 atagacgctg attcgtaact tgccctccat ggactggagg acaagtgatg aacaagcacg
     1801 aatcaaatcc gaagcagagc aactggacgg gcagccgatt ggcgtcggca cgctggcagg
     1861 agttgcacaa ccatctctcg cgccacccgg ccacggccga gtacggctgg gcgtgtcgct
     1921 cttcgctgat ctgcaagtgg cgcgccgcac ccgtggccgt tttgcaggcg acttcgccgg
     1981 tgggttgagc cagtgatcct cgccatccat ccgagctacc cgaatgcgcg tgcctacgtg
     2041 ctcaagctgc atcgcgacgc tcgtccggcc gccggcaagg tcatcggccg cctcgagaac
     2101 gtgaccacgg gcgagcagtt cgtgttcggc agcgccgaag aactcctggc cgcattggca
     2161 cgcgacgtat ccagcacccg attcgaatag aggcaaccat ggacagcaat ctttcagccg
     2221 aagccagcat cgtcgtcacc gtgcaggaca ccgcgagccg tatcgcgctc gcgccgacgg
     2281 actcgttccc cgaggtgttc gccacctcgc gaatgatcgc gctcatggaa ctggccgccg
     2341 cccgcgccat gatgcctttg ttaggcgaag gccagctctc ggtgggcgtt tcgatcaacg
     2401 tgcgccacac ggcggccacg ctgcccggca gccacgtgcg tgcggtggcc acccgcatca
     2461 gcaccgaagc gaagctcatg tatttccgcg tcgaggcttt cgacgaagcg gggctcatcg
     2521 gcaccggcga gcatgcgcga gccatcgtcg ataccacccg cctcatttcc ggcgccgagc
     2581 gccgccgcaa accgatttca aacacccagg ggattcaatg aaaccgttga ttgcattgtt
     2641 aggcgtggcc gcgctgtcgg cctgcgcatc gcttccaggc aaggctccgg cgcccgccct
     2701 caagcctcag gacagcttcg cgagcacaca gatgtttgcc gcgcccgcgg gtgaatggcc
     2761 ggcggcggga tggtggaagg tctatggaga cacccagctc aacgcgctga tcgacgaggg
     2821 cctcgcaggc tcgccttcga tcgccgtcgc cgatgcgcgc ctgcgccgtg cgcgcgccgc
     2881 ccaggacacc agccgcggtt cactcatgcc gcaggtgagc gccagcggct cgatgacgga
     2941 gcagaagcag agctacaact acctgagccc ggccgcgatg actccgcagg gctggaacga
     3001 ctacggccgc gccagcctcg atttttcctg ggagctcgac ttctggggca agaaccgcgc
     3061 cgcgctcgcg gcggccacct ccgaggcggt agccgcgagt gcggatgcgg accagtcgcg
     3121 actcgcgctg tcgtccgcaa tcgcgtcgag ttatgcggaa ctggcgcggc tctattcggc
     3181 gctcgatacg gccatcgcgg cgcgtgatct gcgtaccaag acagccgagc tgttcggcaa
     3241 gagattcgat aacggactcg aaacccgcgg cgggcaacgc caggcggatt cacggcgcgc
     3301 ggcgagtgaa gccgacgtgc tgtcgctaga ggagcagatt gcactgcaga agaaccgcat
     3361 tgcggcgctc atgggcgcgg gcccggatcg cggactttcg attgcgcgtc cgaaggtgga
     3421 cttcacgcgc gcattcgcac tgccttccac gttgtcggtg gatctcatcg gccgccggcc
     3481 ggacctcgtc gcggcgcgcc tgcgtgccga agcggccgcg aagcgcattc acgtgacgcg
     3541 cgcgcagttc tatccgaacg tgaacctgag cgccttcatt ggcgtgcagt cgctcggcct
     3601 cgacttgttg caggagaacg gctccgacat cggcagtgtc gggccggcgt tcactttgcc
     3661 catcttcaac ggtggcaggt tgcgcgggca gttgcgcggc gccgaggccg agtacgccga
     3721 agccgttgcg aattacgacc gtgcgctggt gcaggcgctg caggaggtgg cggatgccgc
     3781 cgtctcgcaa cgcgcgctcg gggcgcagat cggatacatc gatgccgccg tggacgcggc
     3841 gcgcgaatcc tggcgcgtgc agagcaaccg ctacgaaggc gggctcgcca gctacctcga
     3901 cgtgctgtcg gccgaagact atctactcgg caacctgcgc acccaggcgg atctgcgcag
     3961 ccgctcgatg acgctggacg tggcgctgaa cagggcgctg ggcggcggct accagtcttc
     4021 ttcaaatctt tgaaaactcg attacggagg aattgtttta tgacgacgat gaatacagaa
     4081 gtactcggga aggccgacca ggcactcgag caggcggcgg accaggccgc gaaggcaaaa
     4141 cgcaaacgcg ccttgttggt actcggcgcc gtggtgctga cgttggggac cagctatggc
     4201 acctattcgc acttctacgc gtcgaagttc atctcgaccg acaacgcgta cacggcggcg
     4261 gagactgcgc aggtcacacc gtcggtcggt ggcatcgtgc gcgaagtgcg cgtcaccgac
     4321 acgagctacg tccacaaggg tgacgtgctg gtggtgctgg atgacatcga cgcgcgtctc
     4381 gcgctcgcgc aggccgaagc cgagctcggc cgcgcgcagc gccgcgttcg cggctatgtc
     4441 gccaacgatg agagcctgac ggcgcagctc gcggcgcgcg cagccgatga aaagcgcgcg
     4501 aaggcggacc tggatcgcgc agtcatcgac ctgcagcgtc gcgagaacct ggccgaatcg
     4561 ggctcggtgt cgggcgatga gctgaccaag gcgcgcaatg cgtacgaaag cgcccgcgct
     4621 tcgctcgcgc aggcgcgctc caactacacc gcggccattg gctcgcgcaa tgccaatgcg
     4681 gtgctcatcg agaacacaac cgagagcacc aaccccgaag tggcgctggc ccgtgcgcgc
     4741 cgtgaccagg cgcaggtgaa cctggatcgc accgtcatcc gcgcgccggt cgatggcgtc
     4801 gtcgccaagc gttcggtgca ggtgggtcag cagatccagg cgggatcacc cgtgctcgcc
     4861 atcgtgccgc tcgccgaagt gcacgtggac gcgaacttca aggaaggcca gctcgagcgt
     4921 gtacgtatcg gtcagccagt ggaaatcacg tcggatctgt acggcagctc gaagacttac
     4981 cacggcgtgg tcgtgggttt gtcgggcggc acgggcagca cgttcgctgc catcccggcg
     5041 cagaacgcca ccggcaactg gatcaaggtc gttcagcgcg tgccggtgcg aatccgcctc
     5101 gatacggcag agctggaggc gaatccgttg caggtcggat tgtcaatgga agccaccatc
     5161 gatacgcgtt ccaaccaggg ttgatagacg gaagttgaaa gggagacagt gatgaccgca
     5221 tttgaaaagg ccctctccag cgacgcaccc ttgaagggtg cgatgctgtg gctggttgcc
     5281 atcgttctgg ccggcgcaaa tttcatcgcc gtgctcgaca tgaccatcgc caacgtgtcg
     5341 gtgcccaaca tcgcgggtag tttgggcatc agttcgagcc agggcacctg ggtcatcacc
     5401 tgttacgcgg tggcggaagc catcacggtg ccgctcacgg gctggctggc cgcgcgtttt
     5461 ggcgcggtga gggtgttcac caccgccatc ggctgcttcg gcttgttttc cctgttctgt
     5521 ggcctggcca attcgttcgc gatgctggtg gtcgggcgca ttctgcaggg tgtcgcgggc
     5581 ggcccgctga tcccgctgtc gcagacgctg ctgctgcgca tcttcccgaa agagaaggcc
     5641 gccgcggcga ccgcgctttg ggccatgacc acgctggtgg cacccattct cgggccgatc
     5701 cttggtggct atctgtgcga caactacgcc tggccaatga tctttctgat caacgtgccc
     5761 ctcgcgctgg cgatggcgcc gttcgcgctt cggatgctga agcgtttcga aacggccatc
     5821 gtcaggacgc cgatcgatct catcggtctt gcgatcatgg tccttgccgt cggcgcgctg
     5881 cagctcatgc tcgatctcgg caaggagcac gactggttcg agtccgggct cattatttca
     5941 ctcggcatcg tcgccgccat tggcctcgtg gcgttcgtga tatgggagat gggtgagaag
     6001 catccaatcg tgaacttgcg cgtgttccgg catcgtggct tcacgacggc agtgatcgtg
     6061 atcgccgccg gcttcggcgc gatgttcgcg gccaacgttc tcacgccgct ctggctgcag
     6121 agctacatgg gctacacctc gtcctgggcg gggtatgcca cggcctggac cggcgtgctg
     6181 gccgtgttct gcgcgccggc ggcgggtctg ctctccacga aggtggatcc ccgcaagctg
     6241 gtgttcttcg ggctcgtgtg gttaggcggt gtactcgcct tccgcactgg cgcgaccacc
     6301 gacatgacgt tctggcagat atcggggccg ttgctgttga tgggcctcgg cctgccgttc
     6361 ttcttcgtgc cggtcacgac gctggcgctg gcgagcgtcg aagagcacga gaccgcgtcc
     6421 gccgcgggcc tcatgaactt cctgcgcacg ctggcgggtg ccgtggccac ttccgtcgta
     6481 acgacgagtt gggaaagcaa gacgcaggta atgcattcgg agctcgcggg ggtggtggat
     6541 cgcacgggag aagtggcgca tacgctgggc gcgagtggct tcagccccga ggccacgcgt
     6601 caggcgctcg acaacatgac gcagagtcag agcgtgatgc tcgccaccaa tgaaatcatg
     6661 gcgttcgtgt cgctcgcgtt cttcctgtcg gcgatgctca tctgggccgc accgcggccg
     6721 acccgcaagg tggcgttggg agccgcacat tgaaccgagg tagcatctca ccatgactca
     6781 atcactattg acctatcgcg gcaccgttca ctcgtggcac tgcgaccata tgggacacat
     6841 gaacgtcatg tggtacgtgg gcaagttcga cgaggccacg tggtacctcg cagcgatgat
     6901 gggcatgacg gcccaatacc tgaaagacgc gaaacgcggc atggccgccg tcgatcagcg
     6961 catcgcctat cgccgcgaag cgctaccggg cgatgtggtc acgattcgca ccgccatcat
     7021 cgaggtgcgg cagaagagcg tgcgcttcgt gcacgaaatg cggcgtgggg acctcggcga
     7081 cctgctcgcc accatggtcg tcaccggcgt tcatatcgac ggagtggggc ggcgagccac
     7141 ggccttcgac aagcagatcc atgcgaaggc ggaggagatg ataacgcccg acgccgcact
     7201 ctgggacgcc tggccacccg agaaagcgta tctcgactga cggttcagaa taaaaaaggc
     7261 cgcggcgccc ggggcgccgc ggccttctgg ccggcaagat ccgaccgttg acgaactagt
     7321 tggctctcca gcgcacgccg atcgcgaact gccggccatt gagaacattg ccgaagttcg
     7381 tgtgcagcgg gtcgcccacg tccgcgaagt ggtagtacga ctgcgtcaac tcgtcggtaa
     7441 ggttcacggc gtcgaatgag atcgcgaaat tctcattgaa gtcgtagttc agctgcaggt
     7501 cgagactgct ctcgggcctg cgccagatgc cgatcggatt ggcgaagatg cgtgcttcgt
     7561 tgttgttgag gaatttctct cgccacacgt acgagagccg tccgccgaat ccgccgtgtt
     7621 cataggcaag ggacgcattg taggagctgt ccgagaccac gaagaagtcg gtggtctctt
     7681 cgccgatgat gtttccagcg gagtcggtct gcgggatatt ctgcgaggaa tcgaggctcg
     7741 tgtagctgcc caaaatgccg aagccgttga atacgcctgg caactcgggg aagtactgga
     7801 agccgagttc gaagcccttg agtacgccat cagaagcgtt gaccggctgt gtcaccacga
     7861 agtcattggt ggacaggccg gtgttcggaa tcgtgatgcg gcgcgtgagc ggcacgacga
     7921 ggc
//
LOCUS       DASQJS010000298        12809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_186646, whole genome shotgun sequence.
ACCESSION   DASQJS010000298 DASQJS010000000
VERSION     DASQJS010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186646"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..906
                     /locus_tag="VEW08_11930"
     CDS             115..906
                     /locus_tag="VEW08_11930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ41493.1"
                     /translation="MIAADRHLSVPGARLRYRDEGAGPAVVLVHGWTLDLDMWEPQVL
                     LAAQLRIVRFDRRGFGLSSGEASMRADVQDLHALITRLELPRPLLVGMSQGARVVLDF
                     AARHPMIPGALMLDGAPPCAQSDLPMALFRDTARRSGIEAFRKLWADHALTQLVTGDP
                     AIHALRSRILARYRGTDLLAKVEEEHGALDDQALKQIRVPVRIINGECDADTRRHAGQ
                     RLHTALPDSEIVVVANAGHMPNLDAPDDYNRIVSEFARRHLPVAA"
     gene            922..1545
                     /gene="wrbA"
                     /locus_tag="VEW08_11935"
     CDS             922..1545
                     /gene="wrbA"
                     /locus_tag="VEW08_11935"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HYJ41494.1"
                     /translation="MTKILVLYYSSYGHVEQMAHAQAEGARRISGADVVVKRVPELVP
                     EALARSSHFKVDQSAPIADPSEIDRYDGIIFGTPTRFGNMAAQMRNFLDQTGALWYRH
                     ALVGKVGSVFCCTASQHGGQETTITSFHTTLLHLGMVIIGLPYTYKDLATMREVTGGT
                     PYGASSITGAGDEMRMPSALELDMCKYQGEHVTRITRDLAAGRAAAR"
     gene            1591..2754
                     /locus_tag="VEW08_11940"
     CDS             1591..2754
                     /locus_tag="VEW08_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41495.1"
                     /translation="MRDEPAGGSSVAAQAAGTPFRFYDNRQKYLAFVNTCNEKQKVAD
                     RGADELRQIEPMPPALRIFDAGMGDATVLARLMRSAHASWPTVPQLIVAKEISREDVR
                     LGLEKMADRFLEHPATVLVITNLYYADAPALRPRSAADVARLNWRELRLTGNTSHDYA
                     RQIDALGSLLDHGWATRLSETTGNPVYAVPSVLVIYREDHAFLLDRVIPHHDTPGIEY
                     DFILASQPWRARMSAQFKVNKVLAPLTRHLARGGRLLVVQSRGDDPGLEIVRELWADE
                     APFQVDRHRLLGELQAVLGADAAKYSMPDPPDDQAIFRYEMHTLPAEVGDRIGTSMLF
                     AAWNAAIYVNQIEDERLGEVLASGGYLEATQRVLRRHGGLWFNDEAFVVSRLA"
     gene            complement(2765..3751)
                     /locus_tag="VEW08_11945"
     CDS             complement(2765..3751)
                     /locus_tag="VEW08_11945"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HYJ41496.1"
                     /translation="MLLQIVNGDWAASTFLHTFGPSDRLLINRDVLSCGPLPRLTKIS
                     AWQNARLDFWRSTLSHLRDFDFEPSPIDILKNSQRLHGRELPCVWAATGNSDQLMISF
                     VLHLVAVTGGDIGSVHVVQFEKHASSGLRVRGTGDLTPVEMRAHPDARKLTPIELAAY
                     RDAWVAVTSNEPTAVSTFAARHPNAPWYLHEAVAKVLRRYPDRVTGLNFWDRRLLKET
                     RDQGPEAAAVVGHTIAAMFDDGDLVGDLYTFSRLLGMSSSSLPQPLLNMNGSRQQIAR
                     AQVTLTGFGEQVLNGAASAYPVNPIDDWAGGVHLSTTNGNLWFNDDGRIVRA"
     gene            complement(3928..5271)
                     /gene="pmbA"
                     /locus_tag="VEW08_11950"
     CDS             complement(3928..5271)
                     /gene="pmbA"
                     /locus_tag="VEW08_11950"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008268.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HYJ41497.1"
                     /translation="MQEVDGTRIPELKSIVSESLQLARKLGASQAEADVSLQKGLTTT
                     VRLGEVETVEYQRDRGMGITVYFGKRKGSASTADLSSRAVAETVEKACDIARYTAEDE
                     CSGLADPDELARDIPDLDLDHPWDLPPEQAVETARECEAAGRKVDSRITNSEGASVSS
                     HRGVRVYGNSHGFLEGFPSTSHSVSCVLLAQAGDDMQRDYWYASARDARELESSESIG
                     RKAGERAVARLGARKIGTQKARVLYAPEVARGLIGHFLGAVRGSSQYRKSSFLLGASG
                     QQIFPSFVELRERPHIVKALGSSPFDGEGVATRDRDLVNDGVLQGYILGSYSARKLGL
                     KTTGNAGGTHNVLVESKSGGMEIGALLRELGTGLLVTELMGQGVNGVTGDYSRGASGY
                     WVENGVQAFPVHEITIAGNLKDMYRNIAAIGNDIDVRGGVRVGSILISEMMIAGD"
     gene            5287..5802
                     /gene="yjgA"
                     /locus_tag="VEW08_11955"
     CDS             5287..5802
                     /gene="yjgA"
                     /locus_tag="VEW08_11955"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HYJ41498.1"
                     /translation="MRGKDENEEYRGPSKSMLKRDAAAAQDLGTRLIALKEPDLVALN
                     LPETLFDAILLAKRITSRGGLARQRQYIGKLMRGIDPAPIEAALSVESRASAVDAEKH
                     KRIEAWRARLLTEGPKALDDLLKSRPGADRKALQLLITKATSERVDSGSREGASRELF
                     RSIRALFDSIS"
     gene            5802..6293
                     /gene="purE"
                     /locus_tag="VEW08_11960"
     CDS             5802..6293
                     /gene="purE"
                     /locus_tag="VEW08_11960"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HYJ41499.1"
                     /translation="MKPLVGIIMGSTSDWETMRAAAEALDKLGVPYETRVVSAHRTPD
                     LLFEYASSAQGRGIEVIVAGAGGAAHLPGMVASKTTLPVLGVPVQSKALNGLDSLLSI
                     VQMPAGIPVATFAIGVAGATNAGLAAAAILANKRPEIDAALKKFRAEQTAKVLANPDP
                     SKL"
     gene            6290..7396
                     /locus_tag="VEW08_11965"
     CDS             6290..7396
                     /locus_tag="VEW08_11965"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HYJ41500.1"
                     /translation="MTVGIVGAGQLGRMMALAGYPLGLDFLFLDKDAQTPGGQVGPLL
                     AGALTDRSLLGDLSQRCEVVTFDWENIPVEALESLPGKARISPPLRALAAAQDRLSEK
                     RTFELLGIPTTKYAAVDSRESLEVAVRSIGLPGVLKTRRMGYDGKGQFVLRKPADLER
                     AWAELGKSALIYENLVPFDAEVSVVAVRGADGKLAFYPLNLNVHRDGILRLTRAPYGN
                     AALTRQAQRAARKLLTHFQYVGVLTIEFFVKRGRLIANEMAPRVHNSGHWTIEGADTS
                     QFENHVRAIAGLPLGSTRARGHSAMINLVGEMPTRDSLLAEPALHWHDYGKTARPGRK
                     LGHVTICENTAKTRDRRAFKLLSRIDRATWLALR"
     gene            7455..8759
                     /locus_tag="VEW08_11970"
     CDS             7455..8759
                     /locus_tag="VEW08_11970"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYJ41501.1"
                     /translation="MYIENFKLRELPFRLSPDPQFLYLSRAHARAKAYMESTIWFTDG
                     FVVVTGEIGSGKTTLIESFLRQLDSDVVIAQINQTQVNAVEFLQSVLVQFGFSPFKMK
                     KAELIATLNGFLIEQYAAGRKVLLIIDEAQNLSLKVLEEIRMLSGIESTKDKVLRIIL
                     AGQPELNEKLDSPELVQLTQRIRLRFHLGTLSREDLRSYVRHRLDVAGADGREIFAED
                     SFPELYKYTGGVPRLINTLCDTAMMAAFNEDRDFVTPADIASAVSELQWAEFSSRANA
                     IAARSANGVHATGDRPTRALSKLVLSTDGKTVAELHLMPGRKVIGRTPDNDLQIDSKF
                     ISRHHCQLVTGSDGLTVIEDLNSTNGILVRGKRVRRHSLRDGDVVTVGQHEILYVDEQ
                     TGHLVDTHDDLPRVDVNAANEDANEDADDDASSGDAAGAVKI"
     gene            complement(8825..8995)
                     /locus_tag="VEW08_11975"
     CDS             complement(8825..8995)
                     /locus_tag="VEW08_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41502.1"
                     /translation="MRNDTSASGEFRINLPPDVLAEMRRQTGSARNTMQVDGFRPGLF
                     GRLINLFTGRRR"
     gene            9310..9807
                     /locus_tag="VEW08_11980"
     CDS             9310..9807
                     /locus_tag="VEW08_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41503.1"
                     /translation="MKKVAFLLILAVIGTYGVGRVMLGESGAMRFLTSMESMMSEGKA
                     EEVCAMFHEDLEIEISDDSGESPKNISGGKPELCDITKATVAGLSIVPHSMNVDYTEV
                     NARQDWLHPWTGEISYLENRSFSIPGASITLRTVSHDEITLVHTFSGVKLRKLKSEVY
                     KADAT"
     gene            complement(9815..11260)
                     /gene="tldD"
                     /locus_tag="VEW08_11985"
     CDS             complement(9815..11260)
                     /gene="tldD"
                     /locus_tag="VEW08_11985"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HYJ41504.1"
                     /translation="MTSAEPLAIARELILRPGGIDDTLIDNALAQVMSSSVDSADLYF
                     QLSREESWALEDGIVKEGSASIEQGVGVRAVAGEKTGFAYSDEIILPALEEASRAARA
                     IAVRGSDRAMHSVLPTSGHHLYLPIDPVSSFTSSEKVAWLERVDRETRKMDPRVKQVM
                     ASVVAVHEVVLIANSEGHLAADVRPLVRFNVSVIVEQDGRREQGYAGSGGRITLPELV
                     ADDKPLALAREAVRQALVNLDAVPAPAGLMTVVLGSGWPGILLHEAIGHGLEGDFNRK
                     GTSAFAGRIGERVASELCTIVDDGTLSRRRGSLNIDDEGTPTQCTTLIENGVLKGYLQ
                     DKMNARLTGTASTGNGRRESFAHITLPRMTNTYMRPGKTPPEEIIASVQKGIYAVNFG
                     GGQVDITSGKFVFSASEAYLIENGKVGAAIKGATLIGNGPDVLTRVSMVGNDLELDDG
                     VGTCGKEGQSVPVGVGQPTLRIDGLTVGGTG"
     gene            complement(11257..11574)
                     /locus_tag="VEW08_11990"
     CDS             complement(11257..11574)
                     /locus_tag="VEW08_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41505.1"
                     /translation="MTVLVIRRLSGKQDREYPYRVLIDGRQRAEVGDDATVQIGLTPG
                     EHLVSLRVKWCGSRDLPFTIAPGEIVRMQCRPNVRPLLALLYITLWRNDYIALETVKS
                     WTP"
     gene            complement(11591..12406)
                     /locus_tag="VEW08_11995"
     CDS             complement(11591..12406)
                     /locus_tag="VEW08_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HYJ41506.1"
                     /translation="MRVAAIQMTSGHEIDANLEAATRHLEEAARQNVVLAALPENFAF
                     MGQNSGDKRVIAEPDGAGRIQNFLADTARRLKLWIVGGTVPLRQGADGRVAAACLVYK
                     SDGQRAARYDKIHLFDVDVPGSTESHRESAHTVPGRDPRVVETPLGKLGVAVCYDMRF
                     PELFRVMSRQGADVFVVPSAFTVPTGKAHWESLLRSRAIENLCALIAPAQWGVHPNGR
                     ETYGDSMIIDHWGKVLARLPEGQGCVVADIDAAGRMDARARFPSLEHRVIYNT"
BASE COUNT         2374 a         4145 c         4023 g         2267 t
ORIGIN      
        1 cgcgtgccgg gcatcgcgct cagcgcgcag atctagccag cggcgcgcgc cgcttgacgc
       61 tgcgggttcg cgctcgtagc ctgaatgcga aatatttttc ggtattcgaa acccatgatc
      121 gccgccgacc gtcacctgtc agtgcccggc gcccgtctgc gttaccgcga cgaaggcgcg
      181 ggccccgccg tggtgctggt gcatgggtgg acactcgacc tcgacatgtg ggaaccgcag
      241 gtgctgctcg ccgcacagct gcgcatcgtg cgcttcgatc gccgtggctt cggactttcg
      301 tccggcgaag cgtccatgcg tgccgacgtg caggatctgc acgcgttgat caccaggctc
      361 gaactcccca ggccgttgct ggtgggcatg tcgcagggcg cgcgcgtggt cctcgatttc
      421 gcggcgcgcc atcccatgat tcccggcgcg ctgatgctcg acggcgcacc gccgtgcgca
      481 cagtccgacc tgccgatggc actgttccgt gatacggcgc gccgcagcgg catcgaagca
      541 tttcgcaagc tgtgggcgga tcacgcgctg acgcaactcg tcaccggcga cccggcgata
      601 cacgcactgc gctcacgcat cctggcgcgt taccggggca cggacttgct ggcgaaagtc
      661 gaagaagagc acggcgcgct ggacgaccag gcgctcaagc agatccgcgt gccggtgcgc
      721 atcatcaacg gcgaatgcga cgcggacacg cgccggcatg ccgggcagag gctgcacacc
      781 gcattgccgg attctgaaat cgtggtcgtg gcgaacgccg gtcacatgcc gaacctcgat
      841 gcgccggacg actacaaccg gatcgtgagc gaatttgcac gccggcacct gccggtcgcg
      901 gcctgaacca gggagagatt catgacaaag attctggtgc tctattacag cagctatggg
      961 cacgtcgaac agatggcgca tgcccaggcc gagggtgcgc gtcgcatcag cggcgcggac
     1021 gtcgtcgtca aacgcgttcc ggaactcgtg ccggaagccc tcgcgcgcag ctcccatttc
     1081 aaggtcgatc agtccgcacc catcgccgat cccagcgaga tcgatcgata cgacggaatc
     1141 atcttcggca cgcccacgcg cttcggcaac atggcggcgc agatgcgcaa cttcctcgat
     1201 cagaccggag cgttgtggta ccggcacgca ctggtcggca aagtcggcag cgtgttctgc
     1261 tgtaccgcca gccagcacgg cggccaggaa accacgatca cctcgttcca cacgacactg
     1321 ttgcacctgg gcatggtcat catcggattg ccctacacct acaaggacct cgccacgatg
     1381 cgcgaagtga ccggcggcac gccatacgga gcgagctcga tcaccggcgc cggcgacgag
     1441 atgcgcatgc cgagtgcgct cgagctcgac atgtgcaagt accagggcga acacgtcacg
     1501 cgaatcacgc gcgacctggc cgcgggccgc gcggccgcgc ggtagccgga cccggtagcc
     1561 gtgttcgata gataggcagc ggagccagcg atgcgcgacg aaccagcagg cggaagcagc
     1621 gtcgcggcac aggcagccgg cacgcccttc cgcttctacg acaaccgcca gaagtacctg
     1681 gcgttcgtca acacctgcaa cgaaaaacag aaggtggcgg atcgtggcgc cgacgagctg
     1741 cggcagatcg agccgatgcc gccggcactg cgcatcttcg atgccggcat gggtgatgcg
     1801 acggtgctgg cgcggctcat gcgcagcgcc cacgcttcat ggcccacggt tccccagctc
     1861 atcgtcgcca aggagataag ccgcgaggac gtgcgcctgg ggctcgagaa gatggcggac
     1921 cgtttcctgg aacatccggc caccgtcctg gtgatcacca atctgtatta cgccgacgcg
     1981 ccggcattgc gtccgcggag tgcggctgac gtcgcgcgac tcaactggcg tgagctcagg
     2041 ctcactggaa atacgagcca tgactacgcg cgccagatag acgcgctggg atccctgctc
     2101 gatcacggct gggcgacgcg tctcagtgaa accaccggca accccgtgta tgcggtgcca
     2161 tcagtcctgg tgatctaccg cgaggatcac gcgttcctgc tcgaccgtgt catcccgcat
     2221 cacgacacgc cgggcatcga atacgacttc attctcgcct cgcaaccctg gcgcgcgcgc
     2281 atgtcggcgc agttcaaggt gaacaaggtg ctggcgccgc tgacgcggca cctcgcgcgc
     2341 ggtggcaggt tgttggtcgt gcagtcgcgt ggtgatgacc ccgggctcga aatcgtccgc
     2401 gagctctggg ccgacgaggc gccgttccag gtggaccgtc accgcctgct aggcgagctc
     2461 caggccgtac tcggtgcaga cgccgcgaaa tactccatgc ccgacccgcc cgacgaccag
     2521 gcaatcttcc gctacgagat gcacacgctg cccgcagaag tcggcgaccg tatcggcacc
     2581 tcgatgttgt tcgcggcgtg gaatgcggcg atctacgtca accagatcga ggatgagcgg
     2641 ctgggagaag tcctcgcaag cggcggatat ctcgaagcga cgcaacgcgt gttgcgccgg
     2701 cacggcggtc tgtggttcaa cgacgaagcg ttcgtggtga gccggctcgc gtgagcggaa
     2761 ccgctcaggc gcgcacgatg cgtccgtcgt cgttgaacca gagattgccg ttcgtggtcg
     2821 acaagtgaac accaccggcc cagtcgtcga tggggttcac cggatacgct gacgccgcac
     2881 cattcaggac ctgttcgcca aacccggtca gcgtgacctg tgctcgcgcg atctgttgcc
     2941 ggctcccatt catgttcagg agcggttgcg gcaatgaact gctgctcatc ccgagcagcc
     3001 gcgaaaaggt gtagaggtca ccgaccaggt cgccgtcgtc aaacatggca gcgatcgtgt
     3061 ggccgacgac cgccgccgcc tcggggccct ggtcccgcgt ttccttcaag aggcgacggt
     3121 cccagaagtt gagaccggtg acgcgatcgg gataacggcg cagcaccttg gccaccgcct
     3181 cgtgcaggta ccacggagca ttcggatgcc gcgcggcaaa ggtcgagacc gcggtcggtt
     3241 cattcgacgt gaccgctacc cacgcatcgc gatacgccgc gagttcgatg ggcgtgagct
     3301 ttctcgcgtc cggatgcgcg cgcatttcaa cgggcgtgag atcgccggtt ccgcgcacac
     3361 gcagaccgga ggacgcatgt ttctcgaact gaacgacatg caccgaaccg atgtctccgc
     3421 ccgtcaccgc gaccaggtga agcacgaaag aaatcatcag ttgatcgctg ttgccggtgg
     3481 ccgcccagac gcagggcaat tcgcgtccgt gcaggcgttg cgaattcttg aggatgtcga
     3541 ttggcgatgg ctcgaaatcg aaatcgcgca ggtgtgacaa cgtgctgcgc cagaaatcga
     3601 gtcgcgcgtt ctgccaggcg gatatcttgg tgagtcgcgg gaggggcccg caggaaagta
     3661 catcgcgatt gatgagcagc cggtcgctcg gcccgaaggt gtgcaggaag gtgctcgcag
     3721 cccaatcgcc gttcactatc tgcaaaagca cgggatcgaa gtctgacaac gcctgcctcc
     3781 cgcaatgcgt tcaatcgccg gaagtcacac gggaagtatg aagcgaaacg ctgctccgta
     3841 gtgtcggtcc gcgcccagat ccggcagaaa tttttgagaa acaccgcact ctgtttcact
     3901 tgtgccggtg aaatccggga aaggcgtcta gtcgccggcg atcatcattt cacttatcag
     3961 aatgctgccc acacgcaccc caccgcgcac atcgatgtca ttgccgatcg ccgcgatgtt
     4021 cctgtacatg tccttgaggt tgcccgcaat ggtgatttcg tgcaccggaa aagcctgcac
     4081 gccattctcc acccagtacc cggaagcgcc gcgcgagtag tcgccggtca cgccgttcac
     4141 gccctgcccc atgagctcgg tgactaacaa cccggtgccg agctcgcgca ggagcgcgcc
     4201 gatttccatg ccgccggact tcgattccac cagcacgttg tgcgtgccac cggcatttcc
     4261 ggtggtcttg agacccagct tgcgtgcgga gtagctgccg aggatgtagc cctgcaacac
     4321 gccgtcattc accaggtcgc gatcgcgcgt ggccacgccc tcgccatcga acgggctgct
     4381 gcccagcgcc ttcacgatgt gcggccgctc gcgcaattcc acgaacgacg gaaaaatctg
     4441 ctggcccgac gcgccgagca ggaacgagct cttgcgatac tggctgctgc cgcgcacggc
     4501 accgaggaaa tggccgatga gcccgcgcgc cacttccggc gcgtagagca cgcgcgcttt
     4561 ctgggtaccg atcttgcgcg cgccgagccg cgccaccgcg cgttcacccg ccttgcggcc
     4621 gatgctctcg gaactctcga gctcgcgcgc atcgcgcgcc gaggcgtacc agtagtcccg
     4681 ctgcatgtca tcgccggcct gggcgagcag cacgcagctc acgctgtggc tggtggacgg
     4741 aaaaccttcg agaaagccgt gcgagttgcc gtacacgcgc acgccacggt ggctgctgac
     4801 gctggcgccc tccgaattcg tgatgcgcga atcgaccttg cgcccggccg cttcgcattc
     4861 tcgcgcggtt tctaccgcct gctcgggcgg aagatcccag ggatggtcga ggtcgaggtc
     4921 ggggatgtcg cgcgccagct cgtcgggatc cgcaaggccc gagcattcgt cctcggcggt
     4981 gtagcgggcg atatcgcagg ccttttccac ggtttcggcc acggcgcgcg aagacaggtc
     5041 agcggtgctg gcggacccct tgcgcttgcc gaaatacacg gtgatgccca tgccgcgatc
     5101 gcgctggtat tcgacggttt cgacttcacc cagccggaca gtggtagtca gtcctttttg
     5161 caggctgacg tcggcttcgg cctggctggc gcccagcttg cgggcaagct gcaacgattc
     5221 ggaaacgatg ctcttgagct cggggattcg ggtaccgtct acttcctgca tggctagaat
     5281 tgtacgatgc gcggcaaaga cgaaaacgag gaatatcggg gtcccagcaa gagcatgctc
     5341 aagcgtgatg ctgcggcagc ccaggatttg ggaacccgcc tgatcgcgct caaggaaccc
     5401 gacctcgtcg cgctcaacct gcccgaaacc ctgttcgatg ccatcctgct cgcgaaacgc
     5461 atcaccagcc gcggcggact cgcgcgccag cgccagtaca tcggcaagct catgcgcggc
     5521 atcgatcccg cgcccatcga ggcggcgctc agtgtcgaat cgcgcgcctc ggcggtggac
     5581 gccgaaaaac acaagcgcat cgaggcctgg cgcgcgcggc tactcaccga ggggccgaag
     5641 gcgctcgacg acctgctcaa atcgcgtccc ggtgccgatc gcaaggcgtt gcagttgttg
     5701 atcaccaagg cgaccagcga acgcgtcgat tcgggcagca gggaaggtgc gtcgcgcgag
     5761 ctgttccgtt cgattcgcgc tttgtttgat agtatttcgt gatgaaacca ctggttggca
     5821 tcatcatggg ctcgacttcc gactgggaga cgatgcgcgc cgcggctgaa gcactcgaca
     5881 agctcggtgt tccttacgag acccgcgtcg tctccgccca tcgcactccg gacctgctgt
     5941 tcgaatacgc gtcgagtgcg cagggtcgcg gcatcgaagt gattgttgcc ggtgccggcg
     6001 gcgctgccca tctaccgggc atggtggcat ccaagaccac gttgccggtg ctcggcgtgc
     6061 cggttcaatc caaggctctc aacggtctcg actcgttgct gtccatcgtg cagatgcctg
     6121 cgggaattcc cgtggccacc ttcgccatcg gtgtggccgg cgccaccaat gcgggcctcg
     6181 cggcggccgc catcctcgcg aacaagcgtc cggaaatcga cgcggcgctc aagaaattcc
     6241 gcgccgagca gacggccaag gttctcgcca acccggaccc gtcgaagctg tgactgtagg
     6301 aatcgtcggc gccggacagc tcgggcgcat gatggccctc gcgggttatc cactgggcct
     6361 cgacttcctg ttcctcgaca aagatgcgca gactcccggg ggacaggtcg gcccgctgct
     6421 cgcgggcgcc ctcaccgatc ggagcttgtt aggcgatctc agccagcgtt gtgaggtggt
     6481 gactttcgac tgggagaaca ttccggtcga ggcactcgaa agtctgccgg gcaaggcgcg
     6541 catctcgccg cctttgcggg ctctggccgc ggcccaggac cggctcagcg aaaagcgcac
     6601 gttcgaactg ctcggcattc cgaccacgaa atacgcggcg gtcgattcgc gcgaaagcct
     6661 cgaggtcgcg gtcaggtcga tcggcctgcc cggcgtgctc aagacccggc gcatgggcta
     6721 cgacggcaag ggccagttcg tgctgcgcaa gcccgccgat ctcgaacgcg cctgggccga
     6781 gctgggaaag agcgcgctga tatacgaaaa cctcgtgccc ttcgatgccg aagtgtcggt
     6841 cgtcgccgtg cgcggcgccg atggcaagct ggcgttttat ccgctcaatc tcaacgtaca
     6901 tcgcgatggc atcctgcgcc tcacgcgcgc gccatacggc aacgccgccc tgacccgcca
     6961 ggcccagcgc gccgcgcgca agctgttgac gcattttcaa tacgtcggcg tgctcaccat
     7021 cgagttcttc gtcaagcgcg gcaggctcat cgccaacgaa atggcgccgc gggtacacaa
     7081 ctcgggtcac tggaccatcg aaggcgccga caccagtcag ttcgaaaatc acgtgcgggc
     7141 catcgccggc ctgccgctcg gctccacgcg ggcgcggggc cactcggcga tgatcaatct
     7201 tgtaggtgaa atgcccacgc gagactccct gctcgcggag cccgcactgc actggcatga
     7261 ctacggcaag acagcacggc ccggccgcaa gctaggccat gtcacgattt gcgaaaacac
     7321 ggcgaaaacc cgcgaccggc gggcttttaa gctcctttcc aggatcgacc gcgcgacctg
     7381 gctcgcactt cggtaacccc gaataggggg ttccctgccc caaagccagc tttaccgtaa
     7441 tatcggccgc tgcgatgtat atcgagaact tcaagctgcg cgagctgccg ttccggttga
     7501 gcccggaccc gcagttcctg tacctgtccc gcgcccatgc gcgcgccaag gcgtacatgg
     7561 agtcgacgat ctggtttacc gacggattcg tcgtggtcac cggagaaatc ggctcgggca
     7621 aaaccacgct catcgaaagc ttcctgcgcc agctcgattc ggacgtcgtc atcgcgcaga
     7681 tcaaccagac ccaggtcaac gcagtcgaat tcctgcagtc ggtgctggtc cagttcggct
     7741 tctcgccgtt caagatgaag aaagccgagt tgatcgccac cctcaacggt ttcctcatcg
     7801 agcagtatgc cgccggccgc aaggtgctgt tgatcatcga cgaggcacag aatctctcgc
     7861 tcaaggtgct cgaggagatc cggatgctgt ccggcatcga gtccacgaaa gacaaggtgc
     7921 tgcgcatcat tctcgccggc cagcccgagc tcaacgagaa actcgattcg ccggaactgg
     7981 tgcagctcac ccagcgcatc cgccttcgct tccatctcgg caccttgtca cgcgaggacc
     8041 tgcggtccta cgtccggcat cgcctggatg tggctggtgc cgacggtcgc gagatttttg
     8101 ccgaagacag tttcccggaa ctctacaaat acaccggtgg cgtgccgcga ctcatcaaca
     8161 cgttgtgcga caccgcgatg atggccgcat tcaatgagga ccgcgatttc gtgaccccgg
     8221 cggacatcgc ttcggcggtg agcgagctgc agtgggcgga gttctcgtcg cgcgccaacg
     8281 ccattgccgc caggagcgcc aacggcgttc acgcgaccgg cgatcgcccg acccgcgcgc
     8341 tctccaagct ggtgttgtcc accgatggca agaccgtggc cgagctgcac ctcatgcccg
     8401 gtcgcaaggt catcggccgc acaccggaca acgatctgca gatcgacagc aagttcatca
     8461 gccgacatca ctgccagctg gtcacgggca gcgatggcct gaccgtcatc gaagacctga
     8521 acagcaccaa cggcatcctg gtacgcggca agcgcgtacg ccgccattcg ctgcgcgacg
     8581 gtgacgttgt caccgttggc cagcacgaaa tcctgtacgt ggacgaacaa accgggcacc
     8641 tcgtcgacac gcacgacgat ctgcctcgcg tcgacgtgaa tgccgccaat gaagacgcca
     8701 acgaagacgc ggatgacgac gcatcatcgg gagacgcagc aggcgcggtg aaaatctgac
     8761 cccgaaaaga aaaaggcgcg tgagctgcaa gctcacgcgc ctttttttgt ttcgagggtc
     8821 gaggttagcg gcgtcggccc gtgaacagat tgatcaggcg cccgaacagg ccgggacgaa
     8881 aaccatccac ttgcatggtg ttgcgcgcgc tgccggtctg gcggcgcatt tccgcgagca
     8941 cgtccggcgg caggttgata cgaaattcgc cgctggcaga tgtgtcgtta cgcattttcg
     9001 aatggctccg ttgaggatca gttgagaccg cgatggcagt gtggcgaagc atgtgggagc
     9061 catgatgtgc cgggagtcac attgtttcca atgtcgcaaa acgcacactt cattatgtac
     9121 atgtctgatt cgcgtgtagg cgcggagcga ttccttcgct acaaaatgct gacgcacttt
     9181 gacgacatcc ctgtcgaccg cgccccgtga tagtgctgcc gcgaagggct gtcacggtaa
     9241 cgtgaatacc tacgggatcg cccagttatt cccgtgcatg cttcgcacaa tcattcaatt
     9301 ggtgcacaca tgaagaaagt cgcttttctg ctgatactcg cggttattgg cacctacggc
     9361 gtcggccggg tgatgctcgg tgagtcgggt gccatgcgtt ttctcacgag catggaatcg
     9421 atgatgagtg aaggcaaagc cgaggaagtc tgcgcgatgt tccatgaaga cctcgaaatc
     9481 gagatctcgg atgattccgg cgaatcgccg aagaacatca gcggcggaaa gcccgaattg
     9541 tgcgacatca ccaaggcaac cgttgcgggc ctgagcatcg tgccgcattc gatgaacgtg
     9601 gactacaccg aagtgaacgc cagacaggac tggttacatc cctggaccgg cgagatcagt
     9661 tacctcgaga accgcagctt cagcatccct ggcgccagca tcacgctgcg caccgtgagc
     9721 cacgatgaga tcacgctggt tcacaccttc tcgggcgtga aactgcgcaa gctgaagagc
     9781 gaggtttaca aggccgacgc cacctgatcg gtgcctagcc ggttccgccg accgtcaggc
     9841 catcgattcg cagcgtaggt tgtcctacac cgaccggcac gctctgccct tccttgccgc
     9901 aggtgccgac gccgtcgtcg agctcgaggt cattgcccac catcgacacg cgcgtgagca
     9961 catcgggtcc gttgccgatc aaggttgcgc ccttgatggc cgcgccgacc ttgccattct
    10021 cgatgagata ggcctcgctg gccgagaaca cgaacttgcc cgaagtgatg tccacctggc
    10081 cgccaccaaa attcaccgca taaatgccct tctgcacgga cgcgatgatt tcttcggggg
    10141 gagtcttgcc gggacgcatg taggtgttgg tcatgcgtgg cagggtgatg tgggcgaacg
    10201 actcgcgcct gccattcccc gtggacgcgg tacccgtgag ccgcgcattc atcttgtcct
    10261 gcaggtaacc cttgagcacg ccgttctcga tcagcgtggt gcactgcgtg ggggtgcctt
    10321 catcgtcgat gttgagcgat ccgcggcggc gggacagggt gccatcatcg actatggtgc
    10381 agagctccga cgccacgcgc tcgccgatgc ggcctgcgaa cgccgaagtg cccttgcgat
    10441 tgaagtcgcc ctcgagtcca tggccgatgg cctcgtgcaa caggatgccc ggccagccgg
    10501 agccgagcac gaccgtcatg aggccggcgg gcgccggcac cgcatcgaga ttcacgagcg
    10561 cctggcgcac ggcctcacgc gccagcgcca gcggcttgtc gtcggctacg agctccggca
    10621 gcgtgatgcg cccgcccgaa cccgcgtaac cctgctcgcg ccgtccgtcc tgctcgacga
    10681 tgaccgagac gttgaagcgc accagcggtc gcacgtcggc ggcgagatgc ccttcgctgt
    10741 tggcgatcag cacgacctcg tgcacggcaa ccacgctggc catgacctgc ttgacgcgcg
    10801 gatccatctt gcgcgtctcg cggtcgacgc gttcgagcca cgccaccttt tcgctcgaag
    10861 tgaagctgct gaccggatcg atgggtagat agaggtggtg gccgctggtg ggcagcacgc
    10921 tgtgcatggc ccggtcgctg ccgcgcacgg cgatggcgcg cgcagcgcgc gacgcctctt
    10981 cgagcgcggg cagaatgatt tcatccgaat aggcaaaccc ggtcttctcg ccggccacgg
    11041 cgcggacgcc cacgccctgc tcgatgctgg cgctgccctc tttgacaatt ccatcctcca
    11101 acgcccagct ctcttcacgc gacagctgga agtagaggtc cgccgaatcc accgagctcg
    11161 acatgacctg ggcgagcgca ttgtcgatca gcgtgtcgtc gatacccccc gggcgaagga
    11221 tgagttcgcg ggcgatggcg agcggttcgg cggaggtcat ggggtccaag atttcaccgt
    11281 ttccagggca atgtagtcgt ttcgccagag cgtaatgtac aggagcgcca gcaggggcct
    11341 gacattgggg cggcactgca tgcgcacaat ctcgccgggc gcaattgtga acggcaggtc
    11401 acgcgaaccg caccatttga cgcgcaggct gacgagatgt tcgcccggcg tgagtccgat
    11461 ctgcacggtc gcatcgtcgc cgacttccgc gcgctgccgc ccgtcgatca gcacgcgata
    11521 cgggtattcg cgatcctgct tgccggacag acggcggatg acgagtacgg tcacgccccg
    11581 ggcctccgca tcacgtgttg tagatgaccc ggtgctcgag cgatggaaaa cgcgcgcggg
    11641 cgtccatgcg gccggcggcg tcgatgtccg cgaccacgca gccctggcct tccggcaggc
    11701 gcgccagcac cttgccccag tgatcgatga tcatcgaatc accgtaggtc tcgcggccgt
    11761 tcggatgcac gccccattgc gccggcgcga tcagagcgca caggttttcg atggcgcggc
    11821 tgcgcagcag cgattcccag tgcgccttgc ccgtcggtac ggtgaaggcc gagggcacca
    11881 cgaacacgtc cgcgccctgg cgcgacatca cgcggaagag ctccggaaat cgcatgtcgt
    11941 agcaaacggc caccccgagc ttgccgagcg gcgtttcgac cacgcgaggg tcacggcccg
    12001 gaacggtgtg cgccgattcg cgatgcgatt cggtgctgcc gggcacgtcg acatcgaaca
    12061 ggtggatctt gtcgtagcgg gcagcgcgct gtccgtcgga cttgtacacc aggcaggcgg
    12121 ctgcgacacg tccgtcggcg ccctggcgca atggcaccgt gccgccgacg atccataact
    12181 tgagacgcct ggcggtgtcc gccagaaagt tctggatgcg gcccgcacca tcgggctcgg
    12241 caatcacgcg cttgtcgccg ctgttctggc ccatgaatgc gaaattttcc ggcaatgcgg
    12301 cgagcaccac gttctgtcgc gcggcttctt cgagatgacg cgtggccgcc tcgagattgg
    12361 cgtcgatttc gtgcccggag gtcatctgga ttgccgcaac gcgcatgcag gcaatttaat
    12421 gggttggagt cctccggtcg agtttgccgc gacagagaaa gccatcctga ggtgccagca
    12481 aaaggggacg gggctgggcg gtggagcgtc agcgcgcgaa cgtcgcgcct cgatcccgct
    12541 tgcaaagaaa tacgagattg ggagctcaat attcgaggcg cctccccatt tcccagcggc
    12601 ggtggtaaga gaagagacgc acggaattga ggtcttccca cagcgtggag ttacacgcct
    12661 cttttccggg cgaacggagc cgcgacgcgt cggtgcacga tcgagcgacg ggtggccctg
    12721 catgcgatga gcgagtgggc cgggatacct ggcctcgtta ggcacgaagc cgatccctgc
    12781 gtcttcagga ctcttcgcaa gctccaaat
//
LOCUS       DASQJS010000299         4540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3009794, whole genome shotgun sequence.
ACCESSION   DASQJS010000299 DASQJS010000000
VERSION     DASQJS010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3009794"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(345..1514)
                     /gene="prpC"
                     /locus_tag="VEW08_12000"
     CDS             complement(345..1514)
                     /gene="prpC"
                     /locus_tag="VEW08_12000"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HYJ41507.1"
                     /translation="MSTTESNPSGSNTGKPKKSVALSGVAAGNTALCSVGQTGNDLHY
                     RGYDILEVAETCEFEEIAYLLIHGKLPNLPELTAYKTKLKQLRGIPAPVRAILEQLPP
                     ATHPMDVMRTGASALGCVLPEANDHNAAGAREIADHLVASFGSMLCYWYHYSHSSRRI
                     HVETDDDTVGGHFLHLLHRRPPPANWVRAMHTSLNLYAEHEFNSSTFTARVIAGTGSD
                     IYSCITGAIGALRGPKHGGANEVALEIQERYGTPDEAEADIRQRVANKEIVIGFGHPV
                     YTVSDPRNVIIKRVAEKLGREAGDMKRYEVAERIEWIMMNVKRMFANLDWYSAVSYHL
                     MGVPTAMFTPLFVMSRVTGWSAHVIEQREDGKIIRPAANYVGPENRKFVPIDQRP"
     gene            complement(1511..2416)
                     /gene="prpB"
                     /locus_tag="VEW08_12005"
     CDS             complement(1511..2416)
                     /gene="prpB"
                     /locus_tag="VEW08_12005"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016142557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HYJ41508.1"
                     /translation="MSRPTPAQLSAGAKFRAAIAAEKPLQVVGAINAYAARMAEATGF
                     RALYLSGGGVAANSLGLPDLGISTMDDVLIDIRRITEASTLPLLVDADTGWGGAFNIA
                     RTVKSFTRAGAAGMHIEDQVQSKRCGHRPGKEVVPAQEMVDRVKAAVDARPDPSFVIM
                     ARSDALASEGLDKTIERLVACVEAGADAIFPEAITDLAMYRKVRGAVGVPVLANITEF
                     GKTPLYTRDELGSVGVDIVLHCCSAYRAMNLAALKVYQAIRRDGTQKNVVGDMQTRDD
                     LYKFLDYHAYEQKLDQLFASEKEKK"
     gene            complement(2429..3427)
                     /locus_tag="VEW08_12010"
     CDS             complement(2429..3427)
                     /locus_tag="VEW08_12010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HYJ41509.1"
                     /translation="MNTFRAFRIHETGGKITGLFENLTLEALTPGEVVIDVQYSGINY
                     KDALAATGAGRILRRYPLVGGIDLAGRVNSSDDARFSPGDEVLVTGSGLSETFDGGYA
                     AVARVNADSVIAMPSGLDAFRAMALGTAGFTAALSIHRMERNGQEPSRGPIVVTGATG
                     GVGSIAVDMLAARGYEVIAVTGKPESVDYLKSLGARQVLLRGDFQLGSKPLEPTRYAG
                     AIDSVGGEVLAGLTRQVDFWGNIASVGLAGGAELKTTVLPFILRGVNLLGINSSATPR
                     AERLEVWKRISTDLAPRHLAQIAQRTIAFADLPGAFQAYLDGKVTGRTVVKIGSQP"
     gene            3464..>4540
                     /locus_tag="VEW08_12015"
     CDS             3464..>4540
                     /locus_tag="VEW08_12015"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ41510.1"
                     /translation="MKYDYDLFVIGAGSGGVRAARIASQAGARVAVAEEYRIGGTCVI
                     RGCVPKKFLVYGAEFSQMVADAPGYGWTLGPASFDWPTLRDNVQKEVTRLSGIYTSNL
                     AKAGVIAFEERAEIVDAHTVKLMKSGGEVRAERILIATGGSPYLPDGLPGLELGITSN
                     EAFLLKKFPERILIVGGGYVALEFANIFHGLGAKTRILHRGDKLLRGFDDDLRAHMHI
                     EVERGGTQLTLKATLTKLEKAGNAIRATLSNGETVETDAVLYAIGRNPHTEGLGLERA
                     GVKLDEAGAVIVDEFSQSCVESIYAIGDVTNRFNLTPVAIRDGHAFADTIYNKRPTPI
                     DHSSVPCAVFGRPPIGTVGLSEADA"
BASE COUNT          797 a         1419 c         1480 g          844 t
ORIGIN      
        1 ccgtcggcgc cgtcgatggc attcgccgtc tgcgattcac cagcccgcac ccgaatgatt
       61 tctccgatgc ggtcatcgat gcgatggcgg aagtcgatgc agtgtgcgag cacgtgcatc
      121 tcccgatgca gtcggggtcg agcgcgattc tcaggcggat gctccgccga tacacgaggg
      181 agcgatatct cgagtgcgtg gccgagatca gggcggcgat ccccaacgtg gcgctcacaa
      241 ccgacatcat cgtcggcttt cccggagaaa cgggcgacga cttcgccgag actctttccg
      301 cggccgctgc gcggatttgt aatcgtgtga agacatgtgg gtgctcaggg cctctgatcg
      361 atcggcacga atttgcggtt ttcaggcccc acgtagttgg ccgccgggcg gatgatcttg
      421 ccgtcctcac gctgctcgat gacatgcgcg ctccagccgg tcacgcgcga catgacgaac
      481 agcggcgtga acatggcggt gggcacaccc atgaggtgat aactcacagc gctgtaccag
      541 tcgagattgg cgaacatgcg cttgacgttc atcatgatcc attcgatgcg ctcggcgact
      601 tcataccgct tcatgtcacc ggcttcacgg cctaacttct cggcgacgcg cttgatgatc
      661 acgttgcgcg gatcggacac ggtgtacacc ggatgtccga agccgatcac gatttccttg
      721 ttggcaacgc gctggcggat gtcggcctcc gcttcatccg gcgtgccgta gcgctcctgg
      781 atttccagcg ccacttcgtt ggcgccgcca tgcttggggc cacgcagcgc gccgatggcg
      841 ccggtgatgc aggagtagat atccgacccg gtaccggcga tgacgcgcgc cgtgaacgtg
      901 gacgaattga actcatgctc ggcatagaga ttgagcgagg tgtgcatggc gcgcacccag
      961 ttcgcgggcg gcggacggcg atgcagcagg tgcaggaagt gaccgcccac cgtgtcgtcg
     1021 tcggtctcga cgtggatgcg gcggctgctg tgcgagtagt ggtaccaata gcagagcatc
     1081 gagccgaagc tcgctaccag gtgatcggca atttcacgcg cgccagcggc gttgtggtca
     1141 ttggcctcgg gaagcacgca gcccagcgcc gacgcacccg tgcgcatgac atccatggga
     1201 tgagtggccg gcggcagctg ctcgaggatg gcgcgcaccg gggccggaat gccgcgcagt
     1261 tgcttgagct tggtcttgta ggcggtgagc tctggcaggt tcggaagctt gccatggatc
     1321 agtagatagg cgatttcttc gaactcgcag gtctcggcca cctcgaggat gtcgtaaccg
     1381 cggtaatgca ggtcgttgcc ggtctgcccc acgctgcaca gcgcggtgtt gccagccgcg
     1441 acgcccgaga gcgcgacgga ttttttgggt ttacctgtgt tcgacccgga agggttcgat
     1501 tccgtcgtgc tcatttcttc tccttctccg acgcgaacag ctggtcgagc ttctgttcgt
     1561 aggcgtgata gtcgagaaac ttgtagaggt catcgcgcgt ctgcatgtcg ccgaccacgt
     1621 ttttctgcgt gccgtcgcgg cggatcgctt gatacacctt cagcgccgcg agattcatgg
     1681 cgcggtacgc cgagcagcaa tgcagcacga tatccacgcc gacgctgccg agctcatcgc
     1741 gcgtatataa aggtgtcttg ccgaattcgg tgatattcgc cagcactggc acgccgacgg
     1801 cgccgcgcac cttgcgatac atcgcgaggt ccgtgatggc ctcggggaag atggcgtccg
     1861 cgcccgcttc gacgcaggcg acgagtcgct cgatcgtctt gtcgaggcct tcgctcgcca
     1921 gcgcatcgct gcgcgccatg atcacgaatg acggatccgg ccgtgcgtcc acggccgcct
     1981 tcacacggtc gaccatctcc tgcgcgggaa ccacttcctt gcccggacgg tggccacatc
     2041 gcttggactg cacctggtct tcgatgtgca tgccggcagc gccggccctg gtgaaagact
     2101 tcaccgtccg cgcgatgttg aaagcgccac cccagccggt gtccgcatcg actaataagg
     2161 gcagcgtgct ggcctcggtg atgcggcgga tgtcgatgag aacgtcgtcc atggtgctga
     2221 tgccgagatc cggcaagccc agcgagttcg cggcgacacc gccgccggat agatagagcg
     2281 cgcggaatcc ggtggcctcg gccatgcgcg cggcataggc gttgatggcg ccgacgacct
     2341 gcaggggttt ttcggccgcg atggccgcgc ggaacttggc gcccgcggaa agctgggctg
     2401 gcgtgggtcg gctcataggc cgcgaagttt acggctgcga gccgatcttt acgactgtgc
     2461 ggccggtgac tttgccgtcg agatacgcct gaaatgcgcc gggcaagtcg gcgaacgcga
     2521 tggttcgctg cgcgatctgc gcgagatgac gtggagccag atccgtggag attcgcttcc
     2581 acacctcgag gcgctccgca cgcggcgtgg cggaggaatt gatgccgagc aggttcacgc
     2641 cgcgcaggat gaacggcagg accgtggtct tcagttccgc gccgcccgcc aggcccacgc
     2701 ttgcgatgtt cccccagaaa tccacctggc gcgtgagtcc cgcgagcact tcgccgccga
     2761 cgctatcgat ggcgccggca taacgtgtgg gttcgagagg tttgctgccg agctgaaagt
     2821 cgccgcgcag aaggacctgc ctggcgccga gcgacttcag atagtcgacg gactcgggct
     2881 tgccggtgac cgcgatcact tcatagccgc gcgccgccag catgtcgacg gcgatgctgc
     2941 ccacaccgcc cgtggcgccg gtgacgacga tgggaccgcg cgacggttcc tggccgttgc
     3001 gctccatgcg atgaatggaa agcgcggcgg tgaagcccgc ggtaccaagc gccatggcgc
     3061 gaaacgcgtc gagacccgag ggcattgcga tgacactgtc ggcgtttact cgcgcgactg
     3121 ccgcatagcc gccatcgaat gtttctgaaa ggccgctgcc ggtgaccagc acttcgtcgc
     3181 caggtgaaaa gcgcgcatcg tcggacgagt tcacgcggcc ggcgagatcg atgccgccga
     3241 ccagtggata gcggcgcagg attctccccg ctccggtggc cgcgagcgcg tccttgtagt
     3301 tgatgccgga gtactgcacg tcgatcacga cttcgccggg tgtcagcgct tcgagtgtca
     3361 ggttttcgaa aaggccggtg attttcccgc cggtttcgtg gatacgaaaa gccctgaacg
     3421 tattcatctc tattccccca gtcgtgtcgg ctagtatcct gccatgaaat acgactacga
     3481 cctgttcgtg atcggcgcag ggtccggcgg cgtacgcgcg gcacgcatcg cctcgcaagc
     3541 cggcgctcgc gtggcggttg ccgaggaata tcgcatcggc ggcacctgcg tgatccgcgg
     3601 ctgcgtaccc aagaagttcc tggtttacgg cgccgaattc tcgcagatgg tcgccgacgc
     3661 gccgggttac ggctggaccc tgggccccgc ctcgttcgac tggccgacgc tgcgcgacaa
     3721 cgtgcagaag gaagtcacgc ggctctccgg catctacacc agtaacctcg ccaaggccgg
     3781 cgtcatcgcg ttcgaagagc gggctgaaat cgtcgacgcg cacaccgtca agctcatgaa
     3841 atccggcggt gaagtcaggg cggagagaat cctgatcgcc accggcggca gcccctatct
     3901 accggatgga ttgcccggcc tcgagctcgg catcacctcg aacgaagcgt ttctattgaa
     3961 gaagtttccc gaacgcatat tgatcgtggg tggcggctac gtcgcgctcg agttcgcgaa
     4021 tattttccac gggcttggcg cgaaaacccg catcctgcat cgcggcgaca agctgctgcg
     4081 cgggttcgac gacgacctgc gcgcgcacat gcatatagaa gtggagcgcg gcggcacgca
     4141 gctcacgctc aaggccacgc tgacgaagct cgaaaaggcg ggtaacgcca ttcgcgccac
     4201 gctctcgaac ggcgagaccg tggaaacgga cgcggtgctg tacgccatcg ggcgcaatcc
     4261 gcataccgag gggctgggcc tcgagcgcgc cggcgtgaaa ctggacgaag cgggtgcggt
     4321 gatcgtcgac gagttctcgc agagctgcgt ggagtccatc tacgccatcg gcgacgtgac
     4381 caatcgattc aatctcaccc cggtggcgat tcgtgacgga cacgcgttcg ccgacaccat
     4441 ctacaacaag cgtcccacgc cgatcgacca cagctcggtg ccctgcgccg tgttcggccg
     4501 accgccgatc ggaacggtgg gcctgtccga agccgacgcc
//
LOCUS       DASQJS010000300        12651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4265315, whole genome shotgun sequence.
ACCESSION   DASQJS010000300 DASQJS010000000
VERSION     DASQJS010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12651
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4265315"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1601
                     /locus_tag="VEW08_12020"
     CDS             261..1601
                     /locus_tag="VEW08_12020"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HYJ41511.1"
                     /translation="MINRKLLGALAALTTLSCAPSAFAQVDPGVRGGPAGAGHPLPGL
                     TANEEIFFDIGQEDFNEAEGIGDGLGPRFNLDGCGGCHFQPGIGGTSGTVNPQFAIAT
                     AFGARNTVPAFITLNGPIREARLKRTPTGARDGGVHALFVISQRNDGTGNASGCNITQ
                     PNFAAEAANNNLIFRIPTPVFGMGLMEQIEERELVRNLTEGAGTRAQLGISGRLNRND
                     NDGRVTRFGWKAQNISAIVFSGEAYNVEMGITNEVFQTERDETPACQFSQLPNDVTNV
                     DGVTGTDTISAIEKFAFFMRFLGAPIQSPNTPGGSNSITEGFNAFVNTGCTHCHTQQL
                     RTGNSTVAALRNQNVRLFSDIALHNMGPGLADDIVQGLATGDEFRTAPLWGLGQRTFF
                     LHDGRTNNLLTAIQAHRSNANGTYPASEANAVIDRFNALSTTAKQHMLNFLRSL"
     gene            1749..2462
                     /locus_tag="VEW08_12025"
     CDS             1749..2462
                     /locus_tag="VEW08_12025"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HYJ41512.1"
                     /translation="MSYIEGFVAAVPRANKDAYRKHASAALPIFKKLGATRMVENWGD
                     DVPDGKVTDFKRSVKAKPEEDVIFSWLEYPDKATRDRANETLMSDPAMKEMGKTMPFD
                     GQRMIWGGFSPINDSGATSKPGYIDGCLIPVPAANQGAYREMADKHAAILMEYGATRT
                     VDAWGDDVPDGKVTDYKGAVQARKDERVVYSWVEWPSKAARDQGWEKAMADPRMKSMK
                     MPFDGERLIYGGFEPILDA"
     gene            complement(2497..3207)
                     /locus_tag="VEW08_12030"
     CDS             complement(2497..3207)
                     /locus_tag="VEW08_12030"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HYJ41513.1"
                     /translation="MPRQFPRARTLIVEDSAPSLTRVRVWDVPVRLVHWLIVLSVAAA
                     WWTADTGRLDWHQYCGYALLGLVSFRIYWGFFGSSTARFSQFVRGPRTIVSYLKGRWA
                     AVPGHNPLGALSIIALLALMLTQAALGLFAVDVDGIESGPLSAYVSFKTGRVAAHWHC
                     ELFDPLLWLIGLHVAAVIYYVFVRKENLIGAMVHGKRAYPAPQPPEVRSASMLRLLIG
                     VVLAAGLTWMVARAFQFG"
     gene            complement(3174..3644)
                     /locus_tag="VEW08_12035"
     CDS             complement(3174..3644)
                     /locus_tag="VEW08_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYJ41514.1"
                     /translation="MPGKLFLCASSAVVVASFMTAAQAADPKTPAEMQKARHEHYEEL
                     GDAFKAVRDQSKASSPDFAALLKAAEIVNQASINQQQWFPKGTGPEAGKTRALPEIWS
                     KPDDFTAAQKMLSDRAPKLLAAAKAKDLDAVTSAFKELGGACKNCHDNFRAPEH"
     gene            3825..6314
                     /locus_tag="VEW08_12040"
     CDS             3825..6314
                     /locus_tag="VEW08_12040"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HYJ41515.1"
                     /translation="MISLKVPSRTATAFGVACMLVFSASPGRSATDAKNPIAAEFHDV
                     ITPILENHCYECHGDGYDKGKVAFDTLVSDEQILNPDLWLRVLNNTRAGLMPAEKKSR
                     LSAAEQATLERWIKYGVFNIDAASPDPGRVTVRRLNRVEYRNTVHDLLGVDYNTDHEF
                     PPDDTGFGFDNIGDALTMSPMLMEKYVAAAQTVVGDAVPTAPSKPAEQIIKGMAFEGT
                     NAKLKWGKRRLTFNDPANVAVSFKSPLAGTYRVKLDLEVNGAYTPDPGKARLIVKIDG
                     KEIRNQELAYHDEKSFVVESGYKWQPSDHTVTIELQPTVPELKKETIIDLFINKVTVE
                     GPLEKSQWVKTENYDRYFPRPVPTGAKQRRAYATELLTAFAQKAYRRPFAEGDDTGTR
                     LAELAETHYREPGKSFEEGVAHAMAAVLSSPRFLFKLEAPAAHPQSALVADVDEYSLA
                     SRLSYFLWSTMPDEELMNLAARGELRRNLPAQVKRMLADSRAENLARNFTGQWLQARD
                     VEGIASNPREIYIRDAGEEASLRDLRKAWRSQDEVTAKRLAEYIDRLVDAKPDFDNDM
                     RKAMRSETEMYFGYVVREDRPVTELIDSNYVFVNDKLAALYGLPDITGPEMRKVTLPA
                     GSPRGGVLTQGSALFVTSNPDRTSPVKRGLFVLANFLGTPPPPPPANVPALEASEEDF
                     KGHDPVLRDILKVHRENPACASCHNRMDPIGLAFENFNALGMWRDTERKQPIAAQGNL
                     ITGETFNSVAELKRILANDHRQEFYATLSRKLLTYASGRGTEYYDVETIDGIVKRLNA
                     NDGRFSALLMGVVESAPFQKMRIQATATVAN"
     gene            6316..7659
                     /locus_tag="VEW08_12045"
     CDS             6316..7659
                     /locus_tag="VEW08_12045"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HYJ41516.1"
                     /translation="MNDSLAAERHSSFSRRHFFQGLGACIALPAFSSLAHGASRSAPT
                     RMAVVYVPNGAIPAAFWPDGEPGGAFALSPTLAPLAKVHQQLQVISGLNDSSAEAGPD
                     GAGDHARASGTFLTGVRIKKTAGSDIRAGVSIDQVVANQIGHLTRFSSLELTCDAVRK
                     SGDCDSGYACAYEYNLAWRSHNQPLSPEHNPRFVFERLFGSGSPRERVGNLKRRQAEQ
                     KSILDFVLDDARSVNAKLDGRDKQKLDQYLTSVREIETRIDNSTRLKVRNPDTDAPAG
                     IPDSYEEHVALMFDMLALAFQTDSTRVATMLISREGSNRSFADIGIASGHHDLTHHKN
                     SPEIIEKVKLIDRWYMQRFAAFLEKMQSTEDIDGKSLLDHSMILYGSGNADGNRHTHE
                     DLPIVLAGGGSGAMKTGRYVKTGGVPITNLFLSMADAMGAKGVESHGDSTGRLTV"
     gene            complement(7802..9859)
                     /locus_tag="VEW08_12050"
     CDS             complement(7802..9859)
                     /locus_tag="VEW08_12050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HYJ41517.1"
                     /translation="MLSPWQFRALTLPMEASYNSWLVGLSILVAMVVAYTALKLAARV
                     ADAGPSSGRWWLLGGAAAMGMGIWSMHFIGMLAFSVAIPLRYSIFKTLLSLVIAMITS
                     GFALAIASRPHLSLKRLAVGSIVMGAGICAMHYSGMSAIQIVPIITYQPLLVLASIGI
                     AIGASFAALWLAFKLRSGQSRYIGLARAGAAVVMGLAISGMHYTAMAASKLAVGSYCL
                     GGASLDNDWLAGTIGLVTLGILALTLITAVYDAHLVSQTRQDAMRLENVNSALQHGKN
                     LLALATRAAGISSWELDITNRRTLWTENEIESLRAAGVDNRLQPNAVIAMTHPEDKSV
                     MFDAINRAVADNKETCAFRFRVVTPLGDSVHLEAHARIFCDADGKAERILGVSWDVTH
                     QVLQEERKRDLQSQLRDASRDAGMAEVATGVLHSVGNVLNSLGVSAALMQSQLRDSRV
                     GNLQRVASLMGEQGEDLGQFLTNDERGRQMPNYLAQLGENLSAENQRLQTEAAAIAAH
                     VGHIRNIVAAQQTYARRGGVTEAIDVAELLDNAVAIHFADMADVSVRREYEPLSPLTI
                     DRHKLIQIVGNLLSNARHALKDRTDARRELVLRVRRQDSESIVIDVQDSGVGIPADVL
                     GRLFEFGYTTKKDGHGFGLHTSAILAKELNGELVAFSDGMGHGARFSLRLASAAAEMQ
                     KQA"
     gene            9955..11874
                     /locus_tag="VEW08_12055"
     CDS             9955..11874
                     /locus_tag="VEW08_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ41518.1"
                     /translation="MNDPAEQTNSRILIIDDNPAIHQDFEKILAQEPSVSSGMTQVER
                     ILFGETEEPVVQPTFELQFAHQGRQGVTMVERALANSRPFALAFIDMRMPPGWDGLET
                     IQHLWAADPDVQVVICSAHSDYDWADFTERLGHSDRLLVLKKPFEPIEVQQCASALTR
                     KWQSEQVVRRQVHSLEHMVAVRTMGLEAANHQLRHLATHDALTGLPNRVLLDDRLDQT
                     IVLAERDKQQFAVLVLDLDRFKIINDSLGHRAGDELLNQVARRLQSVVTKPDTVARIG
                     GDEFVLVLGSAAERSDAGRIAQRAIDALKAPVRISGVDLHISTSIGIAFYPADGTCAE
                     NLIAHADAAMYCAKQRGRNNLQFFEQGMDIATRERVKLESDLHTALAQQQFVLHYQPK
                     VETATDDFHSVEALIRWQHPERGTIMPEDFIPLAEDCGLINAIGEWVVREACRQCKAW
                     QHEGLPTMRVAVNVSASQFRQGNLLAVIHEAVNDAGLDPRCLELELTESAVMTNPEES
                     ANILGKLSSMGVLVSVDDFGTGYSSMSYLRRFPIDKLKIDRGFVKDLMTRADDASIVQ
                     AIISLAHSLRLKVVAEGVETPEQLLSLKSMGCDQYQGFHFSPPLSATDIGALMRRLRQ
                     SEHGLDESGVRHAAG"
     gene            complement(11966..12388)
                     /locus_tag="VEW08_12060"
     CDS             complement(11966..12388)
                     /locus_tag="VEW08_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41519.1"
                     /translation="MSQSGDTSTSQMSQHALSLRFLTRAQAEVAQMLACLPEEHLAIE
                     PAAIAHIERMAHQMSTTAEAFGFPEIDAVAGAIELMTQPGQRRTVRERIELVTGLLEK
                     ISALSIYVEYELADKEMKRVPEELPMSVALPGFAARRK"
BASE COUNT         2554 a         4105 c         3791 g         2201 t
ORIGIN      
        1 gcatgacgat cacttagacc cagtcaactt gtcaatacaa aaagtgtcga gaagttagga
       61 cgcaccatgt tttgcggcag catgcgtcgg aaaaactaca aacgccgcgt cgcgcgttgc
      121 ttgcgagcaa agagtctcta gatcaaacga tctattccct tcgtggacct gtccacatct
      181 ggaccatatg acctatttca cgaatgccgg cactccatgc agccttcgcc ccgcgcggac
      241 gttcatccag ggagctagcc gtgataaatc gaaaacttct cggggcactc gcggccctga
      301 ccacactgtc gtgtgctcca tccgcctttg cacaggtgga tccaggcgtg cggggcgggc
      361 ccgccggagc aggacatcca cttccaggcc tcaccgccaa cgaagagatc tttttcgaca
      421 tcggacagga ggacttcaac gaagccgaag ggatcggcga cggcctcggg ccgcgtttca
      481 acctcgacgg ttgcggcggc tgccattttc agccgggcat cggcggcact tcgggaacgg
      541 tcaacccgca gttcgccatc gccaccgcat tcggcgcgcg caataccgtg ccggcattca
      601 tcaccctgaa tggcccgatc cgtgaggcgc gcctcaagcg cacacccacg ggcgcacgcg
      661 acggcggcgt gcatgcgctg ttcgtcatca gccaacgcaa tgacggaacc ggcaatgcgt
      721 cgggctgcaa catcactcag ccgaacttcg ccgccgaagc cgccaacaac aacctcatct
      781 tccgtattcc cacgcccgtg ttcggcatgg gactcatgga gcagatcgag gaacgggaac
      841 tcgtccgcaa cctgaccgaa ggcgcgggca cccgggcgca actcggcatc agtggccggc
      901 tcaatcgcaa tgacaacgac gggcgcgtca cgcgcttcgg atggaaagca cagaacattt
      961 cggcgatcgt gttctccggc gaggcgtaca acgtcgagat gggcatcacc aacgaggtgt
     1021 tccagaccga gcgcgacgag acgccggcct gccagttctc gcaactgccc aatgacgtga
     1081 cgaacgtgga cggcgtgacc ggcacagaca ccatcagcgc catcgagaag tttgcattct
     1141 tcatgcgctt cctcggcgcg ccgatccagt cgcccaacac gcccggcggc agcaactcca
     1201 tcaccgaagg gttcaacgcc ttcgtgaaca ccggctgcac gcattgccac acgcagcagc
     1261 tccgcaccgg caactcgacc gtcgcggctc tgcgcaacca gaacgtgagg ctgttctccg
     1321 acatcgcctt gcacaacatg gggccgggac tggcggatga catcgtgcag ggactggcga
     1381 ctggcgatga gttccgcacg gcgccgctgt ggggactggg tcagcgcacg ttcttcctgc
     1441 acgacggcag gactaacaac ctgctgacgg cgatccaggc gcatcggagc aatgcgaatg
     1501 gaacgtatcc ggcctccgag gcgaacgcgg tcatcgaccg attcaatgca ctgtcgacca
     1561 ccgcaaaaca gcacatgttg aatttcctgc gctcattgta gaaggtcggt gccgggtgtg
     1621 gatggggaga ggcactcccc gctttcctca cccggcacct tcagcagtga gcgccctcct
     1681 acgccgcagg aacatgcggt ttctccctgc gtcgaattgg gttcacacca gaaccccagg
     1741 agaaactcat gtcctacatc gaaggatttg ttgctgcagt gcctcgcgcc aacaaggatg
     1801 catatcgaaa gcatgcttca gccgcgctgc cgatcttcaa gaaactcggc gccacacgca
     1861 tggtggaaaa ctggggcgat gatgtgcccg acggcaaagt cacggatttc aagcgatcag
     1921 tgaaggcgaa gcccgaggag gacgtcatct tttcgtggct cgaatacccg gacaaggcca
     1981 cgcgcgaccg cgccaacgaa accttgatga gcgacccggc gatgaaggag atgggcaaga
     2041 cgatgccctt cgatggccag cgcatgatct ggggcggctt ttcacccatc aacgacagtg
     2101 gtgcaacgtc gaagcctgga tatatcgacg ggtgcctgat acccgtgccg gcagcgaacc
     2161 agggcgcgta ccgcgaaatg gccgacaaac acgcggctat cctcatggag tatggcgcca
     2221 cgcgcaccgt ggatgcctgg ggcgacgatg ttccggatgg caaagtcacc gactacaaag
     2281 gtgcggtgca ggccaggaaa gatgagcgcg tggtgtattc ctgggtcgag tggcccagca
     2341 aggcagcgcg tgatcagggt tgggagaagg caatggccga ccctcgaatg aagagcatga
     2401 agatgccctt cgatggcgag cgcctgatct atggaggatt cgagcccatt ctcgatgcct
     2461 gaacgggtaa cgtaaaacct ccacgtggcc cgcgcgttac ccgaactgga atgcgcgggc
     2521 caccatccag gtgagtcctg cggcgagcac gacgccgatc aacagccgga gcatggacgc
     2581 ggagcgcacc tctggtggct gcggtgcggg ataggcacgc ttgccatgaa ccatcgcgcc
     2641 gatcaggttc tctttcctca cgaatacgta gtagatcacc gcggcgacgt gcagcccgat
     2701 caaccacagc agcggatcga acaactcgca atgccaatga gccgcgacgc gcccggtctt
     2761 gaaactcaca taggcagaca gcgggcccga ttcgatccca tccacgtcca ccgcaaacaa
     2821 cccgagcgca gcctgggtga gcatcagggc aagcagcgcg atgatgctga gcgcacccag
     2881 cggattgtgg ccgggcacgg cggcccacct gcccttcagg taactcacga tcgtgcgggg
     2941 tccgcgtacg aactggctga accgcgcggt ggagctgccg aagaagcccc agtagatcct
     3001 gaaactaact agtccgagca gcgcataacc acagtactgg tgccagtcca ggcgcccggt
     3061 gtcggcggtc caccacgctg ccgccaccga caacacgatg agccagtgta cgaggcgaac
     3121 cggcacatcc cagacgcgca cgcgcgtcag ggatggcgcg ctatcctcga cgatcaatgt
     3181 tctggcgcgc ggaaattgtc gtggcaattc ttgcaggctc cgcccaattc cttgaacgcg
     3241 ctggttacgg cgtcgagatc tttggccttc gccgcggcca gcagcttcgg cgcgcgatcg
     3301 gaaagcatct tctgtgccgc ggtgaaatcg tcgggcttgc tccagatctc cggcagtgcg
     3361 cgagtcttgc cagcctcggg tccggtgccc ttgggaaacc actgctgctg attgatcgat
     3421 gcctggttga cgatttcggc ggctttcagc agcgcagcga aatccgggct gctggccttc
     3481 gactggtcac gcacggcttt gaaggcatcg cccagctctt cgtagtgctc gtgacgcgcc
     3541 ttctgcattt cagccggcgt cttgggatcg gccgcctgcg ctgccgtcat gaatgacgca
     3601 acgacaaccg ctgaactcgc acacaggaac agtttgccgg gcataacact catacctcta
     3661 tgtaggaatc aggcgccttt ataaacgatt cccgcaaatg aatggggacc ctggcgagtc
     3721 cgctgctgca ccttcgacct ttggcctaca aatgagccgt gactgacctt acgaaaaccc
     3781 gcaaaatgcc cgcgtttccg cgttcgtgcg aggggctctc tccggtgatt tccttgaagg
     3841 ttccgtcgcg caccgccacc gcattcggcg tggcatgcat gctggttttt tcggcctctc
     3901 ccggccggtc cgcgaccgat gcgaagaacc ccatcgccgc cgaattccat gatgtcatca
     3961 cccccattct cgagaaccat tgctacgagt gccacggcga tggctacgac aagggcaagg
     4021 tggcgttcga tacgctggtg tcggacgaac agatcctgaa tccggacctc tggctgcgcg
     4081 tactcaacaa cacgcgtgcc ggcctcatgc cggctgaaaa aaagtcccgc ctgtccgcgg
     4141 cggaacaggc gacgctcgag cgctggatca aatacggtgt cttcaacatc gacgctgcca
     4201 gcccggatcc aggccgcgtc acggttcgcc gcctcaaccg cgtcgagtac cgcaataccg
     4261 tgcacgatct gctcggcgtg gactacaaca ccgaccacga atttccgccg gacgacacgg
     4321 gcttcggctt cgacaacatc ggtgatgcgc tcaccatgtc gcccatgctc atggagaaat
     4381 acgtcgcggc ggcgcagacc gtcgtgggcg acgccgtgcc gactgcgccc agcaaacccg
     4441 cggagcagat catcaagggc atggcgttcg agggcacgaa cgccaagctc aagtggggca
     4501 aacgcaggct cacgttcaat gatccggcga acgtggccgt gtcattcaaa tcgccgctcg
     4561 ccggcaccta ccgcgtgaag ctcgacctcg aagtgaacgg cgcctacaca ccggatccgg
     4621 gcaaggctcg cctcatcgtc aagatcgacg gcaaggaaat ccgcaaccag gaactggcct
     4681 atcacgacga aaaatccttc gtggtcgaat ccggctacaa gtggcagccc tccgatcaca
     4741 cggtgaccat cgagctgcag ccaaccgtgc ccgaattgaa gaaagaaacc atcatcgatc
     4801 tcttcataaa caaggtgacg gtcgaaggtc cgctggaaaa gagccagtgg gtgaagacgg
     4861 aaaactacga tcgctatttt ccgcgccccg tgcccacggg cgccaaacaa cgccgcgcgt
     4921 atgccaccga gttgctgacc gccttcgcgc agaaggccta tcgccggccc ttcgcggagg
     4981 gggacgacac gggaacgcga ctcgcggaac tcgccgaaac gcactatcgc gagcccggca
     5041 agtcgttcga agaaggtgtc gcacacgcca tggcggcggt gctgtcgtca ccgcggttcc
     5101 tgttcaaact cgaagcgccc gcggcccacc cgcaaagcgc gctcgtggcc gacgtcgatg
     5161 aatattcgct tgcctcgcgc ctctcttatt tcctctggtc gacgatgcct gatgaggaac
     5221 tgatgaatct tgcggcccga ggcgagttgc gccgcaacct gccggcccag gtcaaacgca
     5281 tgctcgcgga ttcacgcgcg gagaatctcg cgcgcaattt cacaggccag tggttgcagg
     5341 cgcgtgacgt agaaggcatc gccagcaatc cgcgcgagat ctacatccgc gacgcgggcg
     5401 aggaagcctc gctgcgcgac ctgcgcaaag cctggcgctc acaggacgaa gtcaccgcga
     5461 aaaggctggc cgaatacatc gaccggctgg tcgatgcgaa gcccgatttc gacaacgaca
     5521 tgcgcaaggc gatgcgcagt gaaaccgaga tgtacttcgg ctacgtcgtc cgcgaagacc
     5581 gtcccgtcac cgaactcatc gacagcaact acgttttcgt caatgacaag ctcgcggcgc
     5641 tttacggcct gcccgacatc accggccccg aaatgcgcaa ggtcactctg ccggcgggca
     5701 gtccgcgcgg cggcgtcctc acccagggca gcgcgctgtt cgtcacctcc aatccggatc
     5761 gcacttcgcc ggtgaaacgc ggcctgttcg tgctcgccaa ctttctcggc accccgccac
     5821 cgccgccgcc cgcgaacgtg ccggcactcg aggccagcga agaggatttc aaaggtcatg
     5881 atcccgtgct gcgagacatc ctcaaggtgc atcgcgaaaa cccggcgtgc gcgtcctgcc
     5941 acaaccgcat ggatcccatt ggcctcgcgt tcgaaaactt caacgcgctg ggcatgtggc
     6001 gcgataccga gcgcaaacaa cccatcgcgg cgcagggaaa cctcatcacc ggcgaaacct
     6061 tcaacagcgt cgccgagctc aagcgtatcc tcgccaatga ccatcgccag gagttctacg
     6121 ccacgctgtc gcgcaaactc ctcacttatg cctcggggcg cggcacggag tattacgacg
     6181 tggaaaccat cgacgggatc gtgaagcggc tcaacgccaa cgacggtcgt ttctcggcat
     6241 tgttgatggg cgtcgtcgag tccgcgccat tccagaagat gcgaattcag gcaacagcca
     6301 cggtagccaa ctgacatgaa cgattcgtta gccgccgagc ggcactcaag tttcagccgc
     6361 cgccacttct tccagggcct gggggcgtgt atcgccctgc cggcattcag ttcgctcgcc
     6421 cacggcgcca gtcgttccgc gcccacgcgc atggccgtgg tgtacgtgcc gaacggcgcg
     6481 ataccggcgg cattctggcc cgacggcgag cccggcggcg ctttcgcgct ctcgcccacg
     6541 ctggcgcctc tcgccaaggt gcatcagcaa ttgcaggtca tttccgggct gaacgactct
     6601 tccgccgaag ccggccccga tggcgccggc gaccatgcgc gcgccagcgg cactttcctc
     6661 accggcgtgc gcatcaagaa aaccgcgggc tcggacatcc gcgccggcgt ttcgatcgac
     6721 caggtggtgg ccaaccagat cgggcatctc acgcgattct cgtcgctcga actcacttgc
     6781 gacgcggtcc gcaagtccgg cgactgtgat tcgggttacg cctgtgccta cgaatacaac
     6841 ctcgcctggc gttcacacaa ccagccgctg tcgcccgaac acaatcctcg ctttgtgttc
     6901 gagcggctgt tcggcagcgg cagcccgcgc gaacgcgtcg gcaatctcaa gcgccgccag
     6961 gccgagcaga aatcgattct cgatttcgtg ctcgacgatg cgcgctccgt caatgcgaag
     7021 ctcgacggcc gcgacaagca gaagctggat caatacctca cgagcgtgcg cgagatcgag
     7081 acgcgcatcg acaactccac gcgactgaag gtcaggaacc ccgacaccga cgcgcccgcg
     7141 ggcatccccg acagctacga agaacacgtg gcgctcatgt tcgacatgct tgcgctcgcg
     7201 ttccagaccg actccacgcg cgtcgccacg atgctcatct cgcgtgaagg cagcaaccgc
     7261 agcttcgccg acatcggcat cgcctccggg caccacgatc tcacacatca caagaacagc
     7321 cccgagatca tcgagaaggt gaagctcatc gaccgctggt acatgcagcg gttcgcggcg
     7381 ttcctcgaaa agatgcagag caccgaagac atcgacggca agtcgctgct cgatcactcg
     7441 atgattctct acggctcggg caatgcggac ggcaatcgcc acacgcacga agacctgccc
     7501 atcgtgctcg ccggcggcgg cagtggtgcg atgaagaccg ggcgctatgt gaagaccggc
     7561 ggagtgccca tcaccaacct gttcctcagc atggccgatg ccatgggtgc aaaaggcgtc
     7621 gagagccacg gcgattccac cgggcgactc accgtctaag cctgcttctg catctctgag
     7681 acggtgccgg gccgggaaac cgggataact tccacatgag ggtgccaggc cggattgaag
     7741 tccgacaact cgacgttgtg cgttcggtga aagttttccc ggtttcccgg cccggcacca
     7801 cttaggcctg cttctgcatc tccgcggcgg cacttgccag ccggagcgag aaccgtgctc
     7861 cgtggcccat gccgtcactg aacgccacga gctcgccgtt caattctttc gccaggatcg
     7921 cgctcgtgtg caggccgaac ccgtggccgt ctttcttggt cgtgtagcca aactcaaaca
     7981 gccggcctaa cacgtcggcc gggattccca ctcccgaatc ctggacgtcg atgacgatcg
     8041 attcggaatc ctgccgtcgc actcgcagca cgagctcgcg gcgcgcatcc gtacggtcct
     8101 tgagtgcgtg acgcgcgttg ctcagcagat tgccgacgat ctggatcagc ttgtggcggt
     8161 cgatggtcaa cggcgagagc ggctcgtatt cgcgccgcac cgatacgtca gccatatctg
     8221 caaaatgtat cgcgacggcg ttgtccagca gctccgccac gtcgatcgcc tcggtgacac
     8281 cgccacggcg cgcgtaggtc tgctgggcag cgacgatgtt gcggatgtgc ccgacgtgcg
     8341 cggcgatcgc cgcggcctcg gtctgcagac gctgattctc ggcgctcagg ttttcaccga
     8401 gttgcgccag gtagttcggc atctgcctgc cgcgttcatc attcgtgaga aattgcccga
     8461 ggtcttcgcc ctgctcgccc atgagcgacg cgacgcgctg caggttgccg acccgcgagt
     8521 cacgcagttg cgactgcatg agcgccgcgg aaacgccgag gctgttcaac acgttgccga
     8581 cgctgtgcag cacgccggtt gcgacctccg ccatgccggc atcgcgcgac gcgtcgcgta
     8641 actgggactg gaggtcccgc ttgcgttcct cctgcaatac ctggtgcgtg acatcccagg
     8701 acacacccag gatgcgttcg gccttgccgt ccgcgtcgca gaaaatcctt gcgtgcgcct
     8761 cgaggtgcac cgagtcaccg agtggcgtca cgacgcgaaa acggaacgcg caggtctcct
     8821 tgttgtcggc cacggcgcgg ttgatggcgt cgaacatcac cgacttgtct tccggatgtg
     8881 tcatcgcgat caccgcattc ggctgcaggc gattgtccac gcccgccgcg cgcagcgatt
     8941 cgatctcgtt ctccgtccac agcgtcctgc ggttcgtgat gtcgagctcc cacgaagaaa
     9001 tgccggcggc gcgggtcgcc aacgcgagca gattcttgcc gtgttgcaag gcggagttga
     9061 cgttctcgag gcgcatcgcg tcctggcgtg tctgcgacac caggtgcgca tcgtagacag
     9121 cggtgatcaa tgtgagcgcc aggattccga gcgtgaccag gccaatggtg cctgcgagcc
     9181 agtcgttatc gagggaagca ccgcccaggc aatacgaacc gaccgcgagc ttcgatgccg
     9241 ccatcgccgt gtagtgcatg ccgctgatcg cgaggcccat gacgacggcc gcgccggcac
     9301 gcgccaggcc gatatatcgg gattgtccgc tgcgcagctt gaatgcgagc cacagcgcgg
     9361 cgaacgacgc cccgatcgcg attccgatgg acgccagtac cagcaaaggt tgataggtga
     9421 tgatcggcac gatctggatc gcggacatgc cggagtaatg catggcgcag atgccagcgc
     9481 ccatgacgat ggacccgacc gccaggcgct tgaggctcag gtggggacgg ctggcgatcg
     9541 ccagcgcgaa tcccgaggtg atcatcgcga tgaccagcga gagaagtgtc ttgaatatcg
     9601 agtaccgcag cgggatcgcc acggagaatg ccagcatgcc gatgaagtgc atcgaccaga
     9661 tgcccatgcc catcgcggct gcaccaccaa gcaaccacca tcgcccggac gacggccccg
     9721 cgtcggcgac gcgcgccgcg agcttcagag ctgtgtaggc cacgaccatg gccacgagga
     9781 tggataggcc gacgagccag gagttgtagc ttgcttccat ggggagggtc agggccctga
     9841 attgccaagg gcttaacagt agcctttggg ggaaaccgtt agtgttaagc ggctatcaat
     9901 tctcggcttc cgggccgcgc ggaaagcacg ttacataacg agagcagcac tccgatgaac
     9961 gaccccgccg aacaaaccaa cagccgcatc ctgatcatcg acgacaaccc ggcgattcat
    10021 caggacttcg aaaaaatcct cgcccaggag ccgagcgtga gttccggcat gacgcaggtc
    10081 gaacggatcc tgttcgggga gacagaagag ccggtggtgc aacccacatt cgagttgcag
    10141 ttcgcgcacc agggcaggca gggcgtgacg atggtcgaac gtgcgctcgc gaatagccgg
    10201 ccgttcgcgc tggcgttcat cgacatgcgt atgccgcccg gctgggacgg actcgagacc
    10261 atccagcatt tgtgggctgc cgatccggac gttcaggtcg tgatctgttc ggcgcactcg
    10321 gattacgact gggccgactt caccgagcga ctgggacatt cggacagact gctggtgctg
    10381 aagaaacctt tcgaacccat cgaagtacag cagtgcgcga gcgcactgac gcgcaagtgg
    10441 cagtccgagc aagtggtgcg gcggcaggtt cactccctcg aacacatggt ggcggtccgc
    10501 accatggggc tggaagcggc caatcaccaa cttcgccacc ttgccactca cgacgcgctg
    10561 accggcctgc ccaatcgcgt gctgctcgac gatcgcctgg accagaccat cgtcctcgcg
    10621 gaacgcgaca agcagcagtt cgcggttctg gtactcgatc tcgatcgctt caagatcatc
    10681 aacgactcgc tgggccatcg tgccggggat gagctcctca accaggttgc gcggcgcctg
    10741 caaagtgtcg tcaccaagcc cgacaccgtg gcacgcattg gtggtgacga attcgtgctg
    10801 gtgctcgggt ccgccgcgga gcggtcggac gccggaagaa ttgcccagcg tgcgatcgat
    10861 gccctgaagg cgccggtcag gatctccgga gtcgacctgc acatctccac cagcatcggc
    10921 atcgcgttct accccgccga tggcacctgc gcggaaaatc tgatcgcgca cgccgatgcg
    10981 gcgatgtatt gcgccaagca gcgcggccgg aacaacctgc agtttttcga gcagggtatg
    11041 gacatcgcga cacgcgagcg cgtcaaactg gagagcgacc tgcacaccgc actcgcgcag
    11101 cagcaattcg tactgcacta tcaaccgaag gtggaaaccg ccacggacga tttccacagt
    11161 gtcgaagcac tcatccgctg gcagcatccc gaacgcggca cgatcatgcc ggaggacttc
    11221 atccccctcg cggaggactg tggcctcatc aacgccatcg gtgaatgggt ggttcgcgaa
    11281 gcctgccgac agtgcaaggc atggcagcac gaagggctgc cgaccatgcg cgtcgcggtc
    11341 aacgtgtcgg catcgcagtt ccgccaggga aatctgcttg ccgtgatcca cgaggcggtg
    11401 aacgacgccg gactcgaccc gcgctgtctc gagctggaac tgaccgagag cgccgtcatg
    11461 accaatccgg aggagtccgc gaacatcctc ggaaagctga gcagcatggg tgtgctggtg
    11521 tcggtcgacg atttcgggac cggctattcg agcatgagct acctgcggcg cttcccgatc
    11581 gacaagctga aaatcgatcg cggcttcgtc aaggacctga tgacgcgcgc cgacgacgcc
    11641 tcgatcgttc aggccatcat ttcactggcg cacagcctca ggctcaaggt agttgccgag
    11701 ggcgtggaga cgcccgagca actcctctcc ttgaagtcca tgggctgcga tcagtaccag
    11761 ggatttcatt tcagcccgcc gttgtccgcg accgacatcg gcgcgctcat gcgccgtctg
    11821 cgccaatcgg agcacggcct cgatgaatcg ggcgttcgcc acgccgcggg ttgatccgcc
    11881 gccagcgcgg cctatttccg tgaaactgtc gcgctgcgtt cgagcgcggc cagcaaccac
    11941 gtgagtccgc gcgtgagccg gcgcgttatt tccggcgcgc ggcaaacccc ggcagggcca
    12001 cggacatggg caactcttcc ggaacccgct tcatttcctt atcggcgagt tcgtactcga
    12061 cgtagatcga cagcgccgat attttttcga ggagccccgt gacgagctcg atgcgctcgc
    12121 gcaccgtgcg gcgctggcct ggctgggtca tgagctcgat ggcgccggcg acagcatcga
    12181 tttcggggaa accgaaggcc tcggcggtag tcgacatctg gtgcgccatg cgttcgatgt
    12241 gcgcgatcgc ggcaggctcg atggccaggt gctcttccgg tagacaggcg agcatctgcg
    12301 cgacttccgc ctgggcgcga gtgagaaaac gcagtgacag cgcgtgctgc gacatctgcg
    12361 acgtgctcgt atcacctgac tgggacactg cgaaccctcc ttggcaaccg acgaaagtct
    12421 tacatgacgc agcgtccaat atcggccgca ctccccgggg ctgcgccttt atcctcccaa
    12481 tctgtcggct gcgtctcact atgcgttcga ccgcgactag ttagtgaaaa cgctttcatg
    12541 gctggccggg agacaaattg tcctgacacc ggtgacaccg cgcctgtcct aacaaccttg
    12601 cgcgacaccg ggcgattccg ttgccccacc tttccaccgg ccggatacgg g
//
LOCUS       DASQJS010000301        15909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2385131, whole genome shotgun sequence.
ACCESSION   DASQJS010000301 DASQJS010000000
VERSION     DASQJS010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15909
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2385131"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VEW08_12065"
     CDS             <1..186
                     /locus_tag="VEW08_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41520.1"
                     /translation="EALMAAASNIAQQAAAAQGGAAGAGPEGSAGPGASAAGGGSKAN
                     DDAVDAEFEEVKDKKKA"
     gene            243..1382
                     /gene="dnaJ"
                     /locus_tag="VEW08_12070"
     CDS             243..1382
                     /gene="dnaJ"
                     /locus_tag="VEW08_12070"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HYJ41521.1"
                     /translation="MSKRDYYKVLDLPRTATEADIKKAYRRLAMKFHPDRNPGDKDAE
                     EKFKEAKEAYEVLCETDKRSIYDQHGHAGIDATRQGGGRGNAGADAFGEMFGEMFGDI
                     FGGGRRGGGRAQVFRGADLKYELEINLGQAVFGHSVEIDVAKLMECDTCGGSGAAKGH
                     KPVTCDTCNGVGQVRISQGFFQLQQPCPKCRGNGTIIKNPCDTCLGQGRVRRSKRLAV
                     KVPAGVDTGDRIRLSGEGEAGRNGGPPGDLYVEVHVSEHEIFERDGEHLSCEVPVSFG
                     TAVLGGTVEVPTLDGHVALKIPAETQSGRVFRLRDKGVKPVRGGARGDLFCRVVVETP
                     VKLSGEQRELLRKFEESLQKDAGKHAPRGESFFEGVKRFFSSEPS"
     gene            complement(1383..2081)
                     /locus_tag="VEW08_12075"
     CDS             complement(1383..2081)
                     /locus_tag="VEW08_12075"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYJ41522.1"
                     /translation="MSLVIAGSVHWWLQRDRHTYLTGETAEFVAIFAPPPARDSSATR
                     SELDELLELQKTRTAAEVTAARADRKTEIRRFYSALGFPAGADPDLPLLRALAARVED
                     DTRPYVRAAKEKFRRLRPYEIEPRMDPCIDNVRGDLSYPSGHANYGYVMAYLLREMVP
                     EREAQLIARADEFARQRMVCGVHFRTDIEAGRKGALWLIAAFNGDRTYRQDWNAAMAE
                     LRAALKLPASPRAR"
     gene            complement(2117..3634)
                     /gene="glpK"
                     /locus_tag="VEW08_12080"
     CDS             complement(2117..3634)
                     /gene="glpK"
                     /locus_tag="VEW08_12080"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003231438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HYJ41523.1"
                     /translation="MGAAPGSTHKFILALDAGTTSSRSLLFDAAGRIVALSQREFTQH
                     FPQPGWVEHDALEIWETQSDTIADALRRAHATARDIAAVGITNQRETTVLWDRATGEP
                     VAPAIVWQDRRTAGDCERMRAAGLEPEIAARTGLLLDPYFSGTKLAWLLDQVPGARAR
                     AERGELAFGTIDSWLLFKLTGGRRHVTDVTNASRTLLLNLRIGEWDERLLELLRVPRA
                     CLPTVIDSCLDTTAAIEIEIDGVKLPVTGIAGDQQAALFGQACFSPGMAKNTYGTGCF
                     ALMNTGNAPQTSRHRLLTTVAWRRRSCQYALEGGVFMGGATVQWLRDGLGMIRSSDEV
                     EALAASVPDTGDVYLVPAFAGLGAPQWDAAARGTILGLTRGTTRAHVARAALESIAFQ
                     TADLIQAMQQDAGRPLSELRVDGGAARNDLLLQFQADLLGVPVLRPANTETTAFGAAA
                     LAGLGAGFWQSQEELAALWTLEKRFEPQMARDEAARRRARWSQAVERSREWALPS"
     gene            complement(3645..4637)
                     /locus_tag="VEW08_12085"
     CDS             complement(3645..4637)
                     /locus_tag="VEW08_12085"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYJ41524.1"
                     /translation="MIRIGILGAARIAPRGIVTPANALLGAEVVAIAARDLERAQDFA
                     AQHSIPMAFGSYAELLARDDIDLVYVALPPSAHLEWCTAALALGKHVLVEKPFASNAR
                     DAERMVAAARAANRHLIEGFHYRFHPLFERTLSALRNGEIGRIRKIEAVFNANLPDTP
                     GELRYIEALGGGALMDLGCYCMHWIRTVAGDEPSVVRASARCATPGVDLDIDAELAFT
                     SGPTATLKCSMQPEDGALSRYLRVHGDAGMLELDNPVSPHSGSTLSIDSASASMPDIV
                     SGGDTTFHFQLQHVIDVIEGRAKPLTGGDDAIGNMRAIDAIYRAAGLKPRGLSS"
     gene            4719..5540
                     /gene="dapB"
                     /locus_tag="VEW08_12090"
     CDS             4719..5540
                     /gene="dapB"
                     /locus_tag="VEW08_12090"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HYJ41525.1"
                     /translation="MTTAILIGATGRMGTAIVRESVRAGVAGNSLDVIAAVASPGSKS
                     AGLDVGEVAGVKALGVRVVDELPPDLAKAQVAIDFSRPDLSLRALNVCRAARVPIVIG
                     TTGHGTEFEARIAAVSRDIAVLVAPNTSIGVAVAQELVRIAARALPAEFDIEIVEAHH
                     KHKLDAPSGTALALADSAARARGLDPNEDTLSGRAGQGARREGEIGIASVRAGDIVGA
                     HTVLFAGIGERLTVGHEATDRSIFANGAIRAAAWLAAQKPGRYTMANVLGLKTTG"
     gene            5790..6977
                     /gene="carA"
                     /locus_tag="VEW08_12095"
     CDS             5790..6977
                     /gene="carA"
                     /locus_tag="VEW08_12095"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HYJ41526.1"
                     /translation="MTVTAVLALEDGTIFNGQSIGAKGNTTGEVVFNTALTGYQEILS
                     DPSYSRQIVTLTYPHIGNTGTTPEDLESPTAYAAGLVVRDVPPLASNWRKNEDLREFL
                     ARHKVVAISGIDTRKLTRILREKGAQAGCIITGEKLDPAAAVRLAKKFPGLKGMDLAK
                     VVTTKRSYQWNEGSLWQPENRPVMRAHQRLHVVAYDYGVKRNILRMLSDRGCRMTVVP
                     AQTTAEEVLALEPDGVFLSNGPGDPEPCDYAIEAIRALVVAELPTFGICLGHQLLGLA
                     SGARTMKMKFGHHGANHPVQDLETGRVFITSQNHGFAVDESDLPSTLKATHRSLFDGS
                     LQGIARIDKPAFSFQGHPEASPGPQEMGAMFDRFIHLMLRRLQQKLRARTAGKPSKQV
                     AEG"
     gene            6994..10233
                     /gene="carB"
                     /locus_tag="VEW08_12100"
     CDS             6994..10233
                     /gene="carB"
                     /locus_tag="VEW08_12100"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HYJ41527.1"
                     /translation="MPKRTDIESILIIGAGPIIIGQACEFDYSGAQACKALKEEGYRV
                     ILVNSNPATIMTDPEMADSIYIEPINWRTLARIIEKEEPDALLPTMGGQTALNTALDL
                     VREGVLARHGVELIAASREAIDMAEDRELFRNAMREIGLECPRAAVAHSLTQAVEIQP
                     SLGFPCVIRPSFTMGGSGGGIAYNREEFEQIVERGLDASPTSEVLIEESVIGWKEFEM
                     EVVRDKNDNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQRMRNASIAVLRKIG
                     VETGGSNVQFAVNPKDGRLLIIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LKNDITGGATPASFEPTIDYVVTKIPRFTFEKFPAANSRLTTQMKSVGEVMAIGRTFQ
                     ESLQKALRGLETGLDGLNEQLSSDQKGAALSEEDRDKLAYELRAPGPNRLLYVADAFR
                     AGWSLERIEELSYIDPWFLAQIEDLVIEEARVREGGAALLETDRLRDLKRKGFSDSRL
                     GALTGAGEEAIRAKRYALNVRPVFKRVDTCAAEFATSTAYLYSTYEEECEALPTNKRK
                     IMILGGGPNRIGQGIEFDYCCVHAALQLREDGFETIMVNCNPETVSTDYDTSDRLYFE
                     PLTLEDVLEVIAKEQPEGVIVQYGGQTPLKLSRALEAAGAPIIGTSPDSIDMAEDRER
                     FQVLVKNLGLKQPANATARTEDQAVALARTVGFPLVVRPSYVLGGRAMEVVFNEEDLR
                     NYMTHAVKVSNDSPVLLDRFLDVAIEVDVDACCDGEDVLIGGIMEHVEQAGVHSGDSG
                     CSLPPNSLTPEIQAELREQTRKLAKALNVIGLMNIQFAIQNGIIYVLEVNPRASRTAP
                     FVSKATGVPLAKIGARVMTGKKMKTMENVTEIIPPYFSVKEAVFPFTKFPEADPILGP
                     EMKSTGEVMGTGRTFGEAYAKAQAASGVTLPRSGVVLISVRERDKPGAVVLARKLVEK
                     GFEIAATDGTARAITDAGVPCKRANKVREGRPHIVDMIKNDEFAMIVNTTEGKTAIRE
                     SNSIRREAVHKRVTYYTTLAGALATCEALDHLDAVEVNRLQDLHKEARS"
     gene            10230..10706
                     /gene="greA"
                     /locus_tag="VEW08_12105"
     CDS             10230..10706
                     /gene="greA"
                     /locus_tag="VEW08_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HYJ41528.1"
                     /translation="MKRTPMTLRGAEALRNELKRLKGEDRPRVIKAIAEARSHGDLSE
                     NAEYHAAREQQGFIEGRIGEIEAKLSTAEVIDTSSLTNTGKVIFGATVHLEDQDDGKR
                     ISYQIVGEDEADIKNGRISITSPIARAIVGKSVGDVVDVVAPGGKHSYEVMEVRYV"
     gene            10751..11821
                     /locus_tag="VEW08_12110"
     CDS             10751..11821
                     /locus_tag="VEW08_12110"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HYJ41529.1"
                     /translation="MKRFAVSVCAVAALAAASVVSAQDWVALQDGKTLDGWKTAERPE
                     SWVVEDGVFVSRGERSHLFYVGKVGPRGKPGDFTNFEFQAEVMTSPGANSGIYVHTKW
                     QGPGWPEAGYELQVINSNPPAEKMNGYIEHKMTGSIYAVRNNWQAPVADNVWFTYRIR
                     VVGKTLQTFINDKLICEYAEGPAPSRPPDKKGRLLGSGTFALQAHDPGSIVKYRNMKV
                     RILPNDAAPPAGLVPIADRELDQLITQASNDNIPLIDLGLSAPDGGSREEFDSQARRY
                     GVLPVRIQDLPQMAGSIAVLVDRDRGPDIELLKSLKARGYRVAFSSGGATSFDEAQLK
                     RRLQAIKDAGLGWKDFWVPGKP"
     gene            11851..12657
                     /locus_tag="VEW08_12115"
     CDS             11851..12657
                     /locus_tag="VEW08_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ41530.1"
                     /translation="MLATAMAMPAAACLNSYSFEIKQLLQKNLRPQALQEIAELERAY
                     KTNKSAEITNDLAVARILFGRVDEAIVLLQELEERHPGKGTTAANLGTAFELAGKDQA
                     ALQWIREGIRRDPKEHFGSEWVHVRILEAKLAIKVDANWLDTHSVMGMSFGDAPQPAE
                     PALPVDHLGHEHSLAETRRAISYQLFERTLLVKPKDRVVADLYLTASDLAFLMFMKET
                     VAERQRQMASALRTGYESALSYGDPRTDLINRRMAELERLIPDPLAVKKP"
     gene            complement(12644..15292)
                     /locus_tag="VEW08_12120"
     CDS             complement(12644..15292)
                     /locus_tag="VEW08_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ41531.1"
                     /translation="MAAQYTRSFGREAAAVYLAAALVVFGTWAASRYLILRKFENIEQ
                     NEVSRSIEVMRKALVTKNTEIETVSRDFARWDDMYSYVSTLDTNFEYQNFSEAGLDEM
                     NVDLVWLLDPKEKLLSSFENDTDEAQYRHPASEAVIAEINRLTPIVRTVLDQEGTLRL
                     VQVNGVPHVIAAHTVLRSDRSGPAGGTLVFARRIGAPEIASVGADTQLNVHFAMLDDR
                     AVVAEKPDLLVALDGRRILRRSDNVIEGQLRLDTIDNRPLAVLYTILPRTVMQNGKQG
                     VSYLVGWVALLIGIVLAAWHAYSGRLRRTSAAAATSETRYRAIFDRAASGIVLFDPNS
                     RRVLDANPQAQRLIGASLDELRRRDVTTIFDTPVSLSAGNPEQPAENRPAQLIRGDDE
                     RRDVEFSIVDLDAGTGRVHAMLLQDISHRREAERRAQEHQLSLQHLATHDALTGLPNR
                     TFLNEELPRLIDEAARRGDSLSILYIDCDNFKNINDSRGHSIGDDYLRSVARRLRDSI
                     ASPDLVVRMGGDEFLVVTRHYGLEPDSAAIAERVAAALKKPVRLGEATYSATTSIGLS
                     VYPRDAMTMSELLRSADIALYEAKARGRDNVQMFDSAMNKRVHTRLALEQDLRSAVNR
                     DAIEIHLQPIVDIRSGRLVSFEALARWNDPRHGSVPPLQFIEVAEASDLIVELGECVF
                     RNVARQIAAWQRAGLKPVPVAVNVSAKQLKRSNLPERLVAIAHEFDIAPHQMEVELTE
                     SVAMQDSEQHVTKLHALRDLGVRVSVDDFGTGYSSLAYLRNLPIDYLKIDRTFVRDMN
                     VDRNDAAIVRAIISMAQSLHLGTVAEGIETREHASQLLALGCSTGQGYFFAKPMPAHE
                     TNELLRTMRGAAPVTTNPTLEIVRVS"
     gene            complement(15408..15707)
                     /locus_tag="VEW08_12125"
     CDS             complement(15408..15707)
                     /locus_tag="VEW08_12125"
                     /inference="COORDINATES: protein motif:HMM:NF014087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HYJ41532.1"
                     /translation="MELSERQRKYLRGLGHALNPVLLIGNAGVTPAVIAEAKRALHDH
                     ELIKVKFRGAERAARDAGLAELAATTESVLLQRIGHTGLYYKRRNDRPGIVIPDA"
BASE COUNT         3019 a         4922 c         5162 g         2806 t
ORIGIN      
        1 gaggcgctga tggcggctgc atcgaacatc gcgcagcagg ccgcggccgc acagggcggt
       61 gcggctggtg caggccctga aggctcggcg ggccctggcg cgagtgctgc cggcggtggt
      121 tcgaaggcaa acgacgatgc ggtcgatgcc gagttcgaag aagtcaaaga caaaaagaag
      181 gcctaagtgg ctgctcgctc cgcgccggca tcggcgcgga gtggtttctc taagggctgg
      241 aaatgtcgaa acgcgattac tacaaggtgc tcgatctgcc gcgcaccgcg accgaggccg
      301 acatcaagaa ggcctaccgt cgtcttgcaa tgaaatttca cccggatcgc aatccgggcg
      361 acaaggacgc tgaagagaaa ttcaaggaag ccaaggaggc ttacgaagtt ctctgcgaga
      421 cggacaagcg ctccatctac gaccagcatg gccatgcggg tatcgacgcg acacgccagg
      481 gcggcggacg tggtaacgcg ggcgccgacg ctttcggtga aatgtttggc gagatgttcg
      541 gagacatttt cggcggcggc cgccgcggcg gtggccgcgc gcaggtgttc cgcggcgcgg
      601 atctcaagta cgagctcgag atcaatctcg gccaggcggt gttcggccac tcggtcgaga
      661 tcgacgtcgc caagctcatg gaatgcgaca cctgcggtgg cagcggcgcc gccaagggcc
      721 acaagccggt cacctgcgac acctgcaatg gcgtcggcca ggtgcgcatt tcccagggct
      781 tcttccagct tcagcaaccg tgcccgaagt gtcgcggcaa cggcacgatc atcaagaatc
      841 cctgcgacac ctgcctgggg caggggcgcg tacgccgctc gaagcgactg gcagtcaagg
      901 tgccagccgg cgtcgacacc ggcgatcgca ttcgcctctc gggcgagggc gaagccgggc
      961 gcaatggtgg tccgccgggc gacctgtacg tcgaggttca tgtcagcgaa cacgagattt
     1021 tcgagcgcga cggcgagcat ctgtcgtgcg aggtcccggt gagtttcggg actgcggtac
     1081 tgggcggcac tgtcgaggtg cccacgctcg atggacacgt ggcgttgaag atcccggcag
     1141 agacgcagtc tggccgcgtc ttccggctgc gcgacaaggg tgtgaagccc gtgcgcggcg
     1201 gcgcacgcgg tgacctgttc tgccgcgtgg tggtggaaac accggtaaaa ctcagcggcg
     1261 agcagcgcga gctgctgcgc aagttcgaag aatcgctgca gaaggatgcc ggcaaacacg
     1321 cgccgcgagg cgagagcttc ttcgaaggcg tgaagcgctt cttttcctca gaaccgtcct
     1381 gactaacgcg cccgcggaga cgcgggcaac ttcagcgcgg cgcgtagctc cgccatcgcc
     1441 gcgttccaat cctgtcggta cgtgcgatcg ccgttgaagg cggcgatgag ccacagcgcg
     1501 cctttgcgcc cggcttcgat gtcagttctg aagtgcacgc cacacaccat gcgttgtcgc
     1561 gcgaattcgt ccgctcgcgc gatcaattgg gcttctcgct ccggaaccat ttcgcgaagc
     1621 aggtaggcca tcacgtagcc gtaattcgcg tggccgctcg gatacgagag atcgccccgc
     1681 acattgtcga tgcacggatc catgcgcggc tcgatttcat agggacgcag ccggcggaat
     1741 ttttccttgg ccgcacgcac gtacggccgg gtgtcatcct ccaccctggc agcgagcgcg
     1801 cgcagcagcg gcaagtcggg atccgctcct gccgggaagc cgagagcact gtagaaacgc
     1861 cggatttccg tcttgcggtc cgcgcgcgcc gccgtgacct cggccgcagt acgggtcttc
     1921 tgcagttcga gcagttcatc gagctcactg cgcgtggctg atgaatctcg cgccgggggc
     1981 ggcgcgaata tggcgacgaa ttcggcggtc tctccagtga gataggtgtg ccggtcccgt
     2041 tgcagccacc agtgaacgct gcctgcaatg acgagggaca aggcgatcgc tgctacgacc
     2101 cgggcggatc tcgacctcat gatggcagtg cccattcgcg ggatcgctcc acggcctggc
     2161 tccagcgcgc gcgccgccgc gccgcttcat cgcgggccat ttgcggttcg aagcgcttct
     2221 cgagcgtcca tagcgccgcc agttcttcct gcgattgcca gaacccggcg ccgagtcctg
     2281 ccagtgccgc cgcgccgaaa gccgtggttt cggtgttggc gggtcggagc accggcacgc
     2341 ctaacaagtc cgcctgaaac tgcagcaaca ggtcgttacg tgcggcgcct ccgtcgacac
     2401 gcaactccga cagtggacgg cctgcgtcct gctgcatcgc ctggatgaga tcggcggtct
     2461 ggaaggcaat gctttccagc gcggcccgcg ccacatgcgc ccgcgtggtg ccgcgcgtga
     2521 gtccgaggat ggtgccgcgc gcagcggcgt cccactgcgg cgctccgaga ccggcaaacg
     2581 ccggaaccag gtatacgtcg cccgtgtccg gcacgctggc cgccagcgct tcgacttcat
     2641 cggagctgcg gatcattccc aggccatcgc gcagccattg cacggtggcg ccacccatga
     2701 acacgccgcc ttcgagcgcg tattggcagg atctccggcg ccaggccacg gtcgtgagca
     2761 ggcggtgtcg cgatgtttgt ggagcgttgc cggtgttcat gagcgcgaag cagccagtgc
     2821 cataggtgtt cttggccatg ccgggactga aacaggcctg gccgaacaac gcggcctgct
     2881 ggtcgccggc gattcccgtg accggtagtt tgacgccatc gatctcgatc tcgatcgcag
     2941 cggtggtatc gaggcaggaa tcgatgaccg tgggcaggca ggcgcgtgga acgcgcagca
     3001 actcgagcag gcgttcatcc cactcgccga ttcgcaggtt cagcagcagg gtacggctcg
     3061 cgttcgtcac gtcggtgaca tgacgtcgac cgcctgtgag cttgaacagc aaccagctgt
     3121 ctatcgtccc gaatgcgagt tcgccgcgct ccgcacgcgc ccgcgcgccg ggaacctggt
     3181 cgagcagcca ggcgagtttg gtaccggaga aatacggatc gagcaggagc ccggtgcggg
     3241 cggcaatctc cggttcgagg ccggccgcgc gcatgcgctc gcagtccccg gcggtgcgcc
     3301 ggtcctgcca gacgatggcc ggcgccaccg gctcgccggt cgcacggtcc cacaggaccg
     3361 tagtctcacg ctggttggtg ataccgaccg cggcgatgtc gcgggcagtc gcgtgagccc
     3421 ggcgaagcgc gtcggcaatt gtgtcgcttt gcgtttccca gatctcgagt gcgtcgtgtt
     3481 cgacccagcc gggttgcggg aagtgttgcg tgaattcgcg ctgcgacagc gcaacgatcc
     3541 ggccggcggc gtcgaacagc agcgaacgcg agctggtggt gcccgcgtcg agcgcgagga
     3601 tgaacttgtg cgtcgatcct ggcgcggcac ccacgttcaa cccatcagga ggacaggccg
     3661 cgaggcttca ggcccgcggc tcggtagata gcgtcgatgg cgcgcatgtt gccgatcgcg
     3721 tcatcgccgc cggtcagggg tttcgcacgc ccttcgatga cgtcgatgac gtgctggagc
     3781 tggaaatgaa aggtcgtgtc gccgccggag acgatgtccg gcatcgaggc gctggcggag
     3841 tcgatcgata gtgtcgaacc ggaatggggt gataccgggt tgtcgagttc gagcattccc
     3901 gcatcgccat gcacgcgcag gtaccgtgac aaggcgccgt cctcgggttg catcgagcat
     3961 ttcagtgtgg cggtggggcc gctggtaaat gccagttcgg catcgatgtc gaggtcgact
     4021 cccggggtcg cgcagcgcgc ggaggccctc acgaccgagg gttcgtcgcc ggcgacggta
     4081 cggatccagt gcatgcagta gcaccccagg tccatgaggg ctccgccgcc cagcgcttcg
     4141 atgtagcgca attcgcccgg tgtgtcgggc aggttggcgt tgaacacggc ttcgatcttc
     4201 cggatcctgc cgatttcgcc gtttcgaagc gccgacagcg tccgctcgaa cagcgggtga
     4261 aaacgataat ggaagccctc gatgagatga cgattggcag cacgcgcggc tgccaccatg
     4321 cgttccgcgt cccgcgcatt gctggcgaac ggtttttcga ccagtacgtg tttgcccagt
     4381 gcgagcgcgg ccgtgcacca ctccaggtgg gccgacggtg gcagggcgac gtacaccagg
     4441 tcgatgtcat cgcgggcgag cagctccgca taactaccga aggccatggg aatcgagtgt
     4501 tgcgcggcga agtcctgcgc acgctcgaga tcccgcgccg cgatggcgac tacttccgcg
     4561 ccgagcagcg cgttggccgg agtcacgatg ccgcgcggcg cgatccgtgc cgcgcccaat
     4621 atgccgatgc gtatcatgga aggcagtctc gcacgactac acgctccgca taaaattttg
     4681 gggcacactg tcggcgtgag atgtgaaagg gagcaccagt gacgacagca atcttgatcg
     4741 gcgccaccgg acgcatgggc acggccattg ttcgtgaatc ggtgcgcgcg ggcgtcgccg
     4801 gcaattctct cgacgtcatc gcggcggttg catcgccggg cagcaagtcc gccgggctgg
     4861 acgtgggtga agtcgccggc gtgaaggcgc tcggcgtgcg cgtcgtcgac gaactaccac
     4921 ccgatctcgc gaaggcacag gtggccatcg acttctcgcg tcccgatctg agcctgcgtg
     4981 cgctcaacgt ctgccgcgcg gcacgcgtgc ccatcgtcat cgggaccacc gggcatggca
     5041 cggagttcga agcgcgcatc gcggcggtgt cgcgcgacat cgccgtgctg gttgcaccta
     5101 acacctccat cggcgtggcg gtggcccagg aactggtgcg catcgcggcg cgtgccttgc
     5161 cggcggaatt cgacatcgag atcgtcgaag cccatcacaa acataaactc gatgccccgt
     5221 cgggcaccgc actggcgctg gccgacagcg ctgccagggc gcgcggactc gatcccaacg
     5281 aagacacgct ttccgggcgg gcgggccagg gtgcgcggcg cgaaggcgag atcggtatcg
     5341 ccagcgtccg tgccggcgac attgtgggtg cccatacagt gctgttcgcc gggattggcg
     5401 agcgcctgac ggtcggccac gaggccaccg accggtcgat ctttgcaaat ggtgcgatac
     5461 gggcggcggc ctggcttgca gcccagaaac ccggccgata cacgatggca aacgtacttg
     5521 gattaaaaac aaccggttag aacacttttc ggttcttttc attagaactt tccgggcgca
     5581 tttctcacga aacgggcaag aagtttcgtg gacaccccgg ggcgcgcttc gtaaaattgc
     5641 cgcctaatcc gaaacgggtt agtccaacgc agggcgggag gatgttcgcg agaactcctc
     5701 ccgctttgtg catgtacgct ccgcggagag agcatccact caattaacgc attttttcgg
     5761 gtccgagcag gctcgagggg agttcccgcg tgactgtaac cgcggttctg gctcttgaag
     5821 atggaacgat cttcaacggc cagtctatcg gcgccaaggg caacactact ggcgaagtcg
     5881 ttttcaatac cgcgctgacc ggttatcagg aaattctttc cgatccgtcg tattcacggc
     5941 agatcgtgac gttgacctat ccacacatag gcaacacggg cactacgccc gaggatctcg
     6001 agtctccgac ggcctacgcc gcggggctgg tagttagaga cgttccgccg ctcgccagca
     6061 actggcgcaa gaacgaggac ctgcgcgaat tcctcgcacg ccacaaggtc gtcgccatct
     6121 ccggcatcga cacccgcaag ctcacgcgca tcctgcgcga gaaaggcgcc caggccggtt
     6181 gcatcatcac cggcgagaag ctggacccgg ccgccgcggt gcgcctggcg aagaaatttc
     6241 cgggcctgaa gggcatggac ctggccaagg tggtcactac caagcgttca taccagtgga
     6301 acgaaggcag cctgtggcag ccggagaacc gcccggtgat gcgtgcccac cagcgcctgc
     6361 acgtggttgc gtacgactac ggcgtgaaac gtaacatcct gcgcatgctc tccgatcgcg
     6421 gttgccgcat gacggtggtg cccgcgcaga ccacggccga agaagtgctg gcgctcgaac
     6481 ccgatggcgt attcctgtcg aacggcccgg gagacccgga accttgtgac tacgccatcg
     6541 aggcgatccg cgcgctggtg gtggcggagc tgccgacgtt cggcatctgc ctcggtcacc
     6601 agttgttagg tctcgccagc ggcgcgcgca ccatgaagat gaagttcggc caccatggcg
     6661 cgaaccaccc ggtgcaggat ctcgaaaccg gccgcgtgtt catcaccagc cagaatcacg
     6721 gattcgcggt cgacgaaagc gatctgccgt ccacgctcaa ggccacgcat cggtcgctgt
     6781 tcgacggctc gctgcagggc atcgcaagga ttgataaacc ggcattcagc ttccagggcc
     6841 atccggaagc gagcccgggg ccgcaggaaa tgggcgccat gttcgaccgt ttcatccacc
     6901 tcatgctgag gcgtcttcaa caaaaactcc gggcccgcac cgcggggaaa ccgtccaagc
     6961 aggtcgccga aggctgaagc agcctaacta ttcatgccaa aacgtacaga tattgaaagc
     7021 atcctgatca ttggcgccgg cccgatcatc atcggccagg cctgcgaatt cgattattcc
     7081 ggcgcccagg cctgcaaggc actcaaggag gagggctacc gcgtcatcct cgtcaattcg
     7141 aatcccgcga ccatcatgac cgacccggag atggccgatt cgatctacat cgaaccgatc
     7201 aactggcgca cgctggcgcg catcatcgag aaagaagaac ccgatgcgct gctgcccacc
     7261 atgggcggtc agaccgccct gaacaccgcg ctcgacctgg tgcgcgaagg cgtgctggcc
     7321 cgccacggcg tcgagctcat tgccgcgtcg cgcgaagcca tcgacatggc cgaggaccgc
     7381 gagctgttcc gcaacgccat gcgcgagatc gggctcgagt gtccgcgtgc cgcggtggcc
     7441 cattcgctca cgcaggccgt cgagattcag ccgagtctcg gcttcccctg cgtcatccgc
     7501 ccgtcattca ccatgggcgg gtcgggcggc ggcatcgcct acaatcgcga agagttcgaa
     7561 cagatcgtcg agcgcggcct ggatgcctcg cccacttccg aagtcctcat cgaagaatcg
     7621 gtgatcggct ggaaagaatt cgagatggag gtcgttcgtg acaagaacga caactgcatc
     7681 atcgtctgct cgatcgaaaa cctcgatccc atgggtgtgc acaccggcga ctcgatcact
     7741 gtcgcgccgg cgcagactct cacggacaag gaatatcagc gcatgcgcaa cgcgtcgatc
     7801 gcggtgctgc gcaagatcgg agtcgagacg ggtggctcga acgtccagtt tgcggtgaac
     7861 ccgaaggatg gacggctgct gatcatcgag atgaatccgc gcgtctcgcg ctcatcggcg
     7921 ctggcatcca aggccacggg atttccgatc gccaagatcg ccgcgaagct cgccgtgggt
     7981 tacacgctcg atgagctcaa gaacgacatc accggtggtg caacgccggc ttccttcgag
     8041 ccgaccatcg attacgtggt caccaagatt cctcgcttca cgttcgagaa attcccggcg
     8101 gcgaattcac ggctcacgac gcagatgaag tcggtcggcg aggtcatggc catcgggcga
     8161 acgtttcagg aatcgctgca gaaggcgctg cgcgggctgg agaccgggtt ggacggtctc
     8221 aacgaacagc tgtcttctga tcagaaaggg gccgcgctct ccgaagaaga ccgcgacaag
     8281 cttgcctacg aactgcgcgc tcctgggccg aatcgcctgc tgtatgtcgc ggacgcgttc
     8341 cgtgcgggct ggtcgctgga acgcatcgaa gaactttcat acatcgaccc ctggtttctc
     8401 gcgcagatcg aagatctcgt catcgaggaa gcccgcgtgc gcgaaggcgg cgcggccctg
     8461 ctcgaaaccg atcgccttcg cgacctcaag cgaaagggat tctccgatag ccgtctcggt
     8521 gccctcacgg gcgccggcga agaggcgatt cgtgccaagc ggtacgcact caacgtccgc
     8581 ccggtgttca agcgggtgga cacctgcgcg gcggaattcg ccacgtccac cgcctatctg
     8641 tattcaacgt acgaagaaga gtgcgaggcg ctgcctacca acaagcgcaa gatcatgatc
     8701 ctgggtggcg ggccgaaccg cattggccag ggcatcgaat tcgactactg ctgcgtgcac
     8761 gccgcgctgc agctgcgcga agacggcttc gaaaccatca tggtcaactg caatccggag
     8821 accgtttcca cggactacga cacctcggat cggctgtact tcgagcccct gacgctcgag
     8881 gacgtgctgg aagtgatcgc caaggaacag ccggaaggcg tcatcgtgca atacggtggc
     8941 cagacgccgc tcaaattgtc gcgtgcgctc gaggctgcgg gcgcccccat catcggcact
     9001 tcgccggatt ccatcgacat ggcggaagac cgcgagcgtt tccaggtgct ggtgaagaat
     9061 ctcgggctca aacaacccgc gaacgccacc gcgcgcaccg aggaccaggc cgtggcgctg
     9121 gcgcgcaccg tggggttccc gctggtcgtg cgtccatcgt acgtgctcgg cggccgcgcc
     9181 atggaagtgg tcttcaatga agaagacctg cgcaactaca tgacacacgc ggtgaaggtt
     9241 tccaacgaca gccccgtgct gctggaccgc tttctcgatg tcgccatcga agtggacgtc
     9301 gatgcctgct gcgacggcga agacgtgctt atcggcggaa tcatggaaca cgtggagcag
     9361 gccggtgttc actccgggga ttccggatgt tcattgccgc cgaacagtct cacgccggag
     9421 atccaggcgg aactgcgcga acagacccgc aaactcgcca aggcgctgaa cgtcatcggt
     9481 ctcatgaaca tccagttcgc gattcagaac ggcatcatct acgtgctcga ggtgaatccg
     9541 cgcgcctcgc gtaccgcgcc gttcgtctcc aaggccacgg gcgtgccgct cgccaagatc
     9601 ggcgcgcgcg tcatgaccgg caagaagatg aagacgatgg aaaacgtcac cgagataatt
     9661 ccgccttact tctcggtgaa ggaagccgta ttcccgttca cgaagttccc cgaagctgac
     9721 ccgatcctcg gccctgaaat gaagagcacg ggcgaggtga tgggcaccgg ccgcacgttc
     9781 ggcgaggctt atgccaaggc gcaggctgcg tcgggcgtca ccttgccgcg atcgggtgtg
     9841 gtgctcatca gcgtgcgtga gcgcgacaag cccggcgccg tggtgctcgc aaggaagctg
     9901 gtggagaaag gcttcgaaat tgccgccacc gacggcaccg cgcgcgcgat caccgacgcc
     9961 ggagtaccct gcaagcgcgc caacaaggtg cgcgagggcc ggccgcacat cgtcgacatg
    10021 atcaaaaacg acgaattcgc gatgatcgtg aacaccacgg agggcaagac cgcgatccgc
    10081 gagtcgaact ccattcgtcg tgaagcagtg cacaagcgcg tcacctacta cacgacgctc
    10141 gccggggcgc tcgcaacctg cgaagctctc gatcacctgg atgcagtgga agtgaaccgg
    10201 ctccaggacc tgcacaaaga ggccaggtca tgaaacggac tccgatgacg ttgcgaggcg
    10261 ccgaagcgct gcgcaacgaa ctcaagcggc tcaaaggcga ggaccgcccg cgcgtcatca
    10321 aggcgattgc cgaggcgcgc agtcatggcg atctgtccga gaacgcggaa taccacgcgg
    10381 cgcgtgaaca gcagggcttc atcgagggcc gcatcggtga aatcgaagcc aagctgtcga
    10441 ccgccgaagt catcgacacc tcctcgctca ccaataccgg caaggtgatt ttcggggcga
    10501 cggtccatct cgaagaccag gacgacggca agcgcatctc ctaccagatc gttggtgaag
    10561 acgaggccga tatcaaaaac ggacgtattt ccatcacctc acccatcgcg cgcgccatcg
    10621 tcggcaagag cgtgggcgac gtggtcgacg tggtcgcccc gggtggaaaa catagttacg
    10681 aagtgatgga agttcgctac gtttagctgg ctggctcgcg aaagaacaag aacaacgagg
    10741 gggaggggcc atgaagcgat tcgcggtatc ggtctgcgcg gtagcggcat tggccgcggc
    10801 atctgtggtg tcggcacagg actgggtcgc cctacaggac ggcaagaccc tggacggttg
    10861 gaagacagcc gagcgtcctg aatcctgggt tgtcgaagac ggcgttttcg tctcccgcgg
    10921 tgagcgctcg cacctgttct acgtgggcaa ggtcggccct cgcggcaagc ctggcgactt
    10981 cacgaacttc gaattccagg ccgaggtgat gacttcaccc ggcgccaaca gcggcatcta
    11041 cgttcatacg aagtggcagg gcccgggctg gcccgaggcc ggctacgaac tgcaggtcat
    11101 caattccaat ccgccagccg aaaagatgaa cggttacatc gaacacaaga tgaccggcag
    11161 catctacgcg gtgcgtaaca actggcaggc gcccgttgcc gacaacgtgt ggttcaccta
    11221 tcgcatcagg gtcgtcggca agactctgca gacgttcatc aacgacaagc tgatttgcga
    11281 gtacgcggaa ggtccggcac cttcccgtcc gcccgacaag aaggggcggt tgttaggctc
    11341 cggcactttc gcgctgcagg cgcacgatcc gggcagcatc gtgaaatacc gcaacatgaa
    11401 ggtgcggata ctaccgaacg atgccgcgcc gcccgcgggc ctcgtgccga tcgccgaccg
    11461 ggagctcgat caactcatta cgcaggcatc gaacgacaac attccgctca tcgatctcgg
    11521 gttgtcggcg cccgacggtg gttcgcgcga agagttcgac tcccaggcgc gccgctacgg
    11581 cgtcttgcct gtgcgcatcc aggatctgcc gcagatggcg ggctccatcg ccgtgctcgt
    11641 cgatcgcgac cggggcccgg acatcgaact actgaagagt ctcaaggcca ggggatatcg
    11701 cgtggcattc agttccgggg gcgctaccag ctttgacgaa gcgcagttga agcgccgcct
    11761 gcaggccatc aaggacgcgg gcctcggctg gaaagacttc tgggtgcccg gcaagccgtg
    11821 atgctcggtt tgatccgcgc aggcattttt gtgctcgcca cagcgatggc gatgccggcg
    11881 gccgcctgcc tcaacagcta ttcattcgag atcaaacagc tgttgcagaa gaacctgcgc
    11941 ccccaggcgc tgcaggaaat cgcagaactg gaacgcgcat acaagaccaa caaatcggct
    12001 gagatcacca acgacctggc cgtggcgcgc atcctgttcg ggagagtcga tgaggccatc
    12061 gtgctgctcc aggagctcga agagcggcat cccggcaagg gcacgacggc agccaacctg
    12121 ggcacggcat tcgagctcgc gggcaaggat caggcggcgc tgcagtggat tcgcgaggga
    12181 atacggcgcg accccaagga gcattttggc tctgagtggg ttcacgtgcg catcctcgaa
    12241 gccaaactcg cgatcaaggt tgacgccaac tggctcgaca cgcacagcgt gatggggatg
    12301 tcgttcggcg acgcgccgca gccggccgaa cctgccttgc cggtggatca tctgggccac
    12361 gagcattccc tcgcggaaac caggcgcgcg atttcatatc aactcttcga gcgcaccttg
    12421 ttggtgaagc ccaaggatcg cgtggtcgcg gatctgtacc tgaccgccag cgatttggcc
    12481 ttcctgatgt tcatgaagga aacggtcgcg gagcgtcagc gccagatggc gagtgcattg
    12541 cgcacgggtt acgagagtgc tttgtcgtac ggagatccgc gtacggatct catcaatcgc
    12601 cggatggcgg aactggagcg cctgatacca gaccccctcg cggtcaagaa accctgacga
    12661 tctccagcgt cggattcgtg gtgaccggcg ccgcaccgcg catcgtgcgc agcaattcgt
    12721 tggtttcatg cgccggcatc ggcttggcga aaaaatatcc ctgaccggtc gaacagccga
    12781 gtgccagcaa ctgcgatgcg tgctcgcgtg tctcgatgcc ctccgccacg gtgccgaggt
    12841 gcaggctctg cgccatgctg atgatggcgc gcacgatcgc ggcatcgttg cgatcgacgt
    12901 tcatgtcgcg cacgaaggtg cggtcgatct tgaggtagtc gatgggcagg ttgcgcaggt
    12961 aggccaggct cgaataaccc gtgccgaagt cgtcgaccga aacgcgcacg ccgagatcgc
    13021 gcagcgcgtg cagcttggtt acgtgctgct cggaatcctg catggcgacg ctttcggtga
    13081 gctccacttc catctggtgc ggcgcgatgt cgaactcatg ggcgattgcc accaggcgtt
    13141 ccggcaggtt cgagcgcttg agctgcttgg cggagacgtt gacggccacg ggaaccggct
    13201 tgagcccggc ccgctgccac gcggcgattt gccgcgcgac gttgcggaac acgcattcgc
    13261 cgagctcgac gatgaggtcc gaagcttcgg ccacttcgat gaattgcagc ggcggcacgg
    13321 aaccatgccg cggatcgttc cagcgcgcca gcgcctcgaa actcaccagg cgtccgctgc
    13381 ggatgtcgac gataggctgc aggtggatct cgatggcgtc gcggttcacc gcgctgcgca
    13441 ggtcctgctc gagcgcgagc cgggtgtgca cgcgtttgtt catcgccgaa tcgaacatct
    13501 gcacgttgtc gcggccgcgt gccttggctt cgtacaacgc gatgtcggcc gaacgcagca
    13561 gctcgctcat cgtcatggcg tcgcgcgggt agacagaaag tccgatgctg gtggtggcgg
    13621 agtaggtggc ttcgcccagg cgcaccggct ttttcagcgc ggcggcaacc cgctcggcga
    13681 tggccgcgct gtcgggttcg aggccgtagt ggcgcgtgac gacgagaaat tcatcaccgc
    13741 ccatgcgcac gacgagatcg gggctcgcga tggaatcgcg cagtcttcgt gcgaccgaac
    13801 gaagatagtc gtcaccgatc gagtgcccgc gcgaatcgtt gatgttcttg aagttgtcgc
    13861 agtcgatgta aaggatggac agcgagtcgc cccgacgtgc ggcttcgtcg atgagccgcg
    13921 gaagctcttc gttgaggaaa gtgcggtttg ggagccccgt caacgcgtcg tgcgtggcca
    13981 ggtgctgcag cgacaactgg tgttcctgcg cgcggcgctc ggcctcacgg cgatgcgaga
    14041 tgtcctgcag cagcatggcg tggacgcggc cggtgccggc atccagatcc acgatcgaga
    14101 actccacgtc gcgccgttca tcgtcgccgc ggatcagctg cgccgggcgg ttctcagcgg
    14161 gttgttcggg attgccggct gacaacgaca ccggcgtgtc gaaaatcgtg gtgacgtcgc
    14221 gacggcgcag ttcgtcgagc gaggccccga tgagacgttg cgcctgcgga ttggcatcca
    14281 ggacccggcg cgaattggga tcgaacagca cgatgccgga cgcggcgcga tcgaagatgg
    14341 cgcgatagcg ggtttcactg gtggccgctg ccgcggatgt gcggcgcagc cggccgctgt
    14401 aggcgtgcca ggcggcgagg acgatgccga tgagcaacgc gacccacccg acgagataac
    14461 tcacaccctg cttgccgttc tgcatcacgg tgcgcggcag gatggtgtac agcacggcga
    14521 gcggacgatt gtcgatggtg tcgaggcgca gctggccctc gatgacgttg tccgaacgtc
    14581 gcaggatgcg gcggccatcg agcgccacca gcaggtccgg tttctcggcc acgaccgcgc
    14641 gatcgtcgag catggcgaaa tgcacgttga gctgcgtatc ggcgccgacg ctggcgattt
    14701 ccggggcgcc gatgcgacgc gcgaacacca gggtgccgcc ggcggggccg ctgcgatcac
    14761 tgcggagtac cgtgtgggcg gcgatgacgt gcggcacacc attgacctgc accaggcgca
    14821 gcgtgccttc ctgatcgagc acggtgcgca cgatcggcgt cagccgattg atctcggcaa
    14881 tgaccgcttc gctcgcggga tggcggtact gcgcttcgtc ggtgtcgttc tcgaaagacg
    14941 acagcagttt ttctttcggg tcgagcagcc agaccaggtc gacgttcatt tcgtcgaggc
    15001 cggcctcgga gaagttctgg tactcgaaat tcgtgtcgag agtcgacaca tacgagtaca
    15061 tgtcgtccca gcgcgcgaag tcgcgcgaga cggtttcgat ttcagtgttc ttcgtgacca
    15121 gcgccttgcg catgacctcg atgctgcgtg aaacctcgtt ctgctcgatg ttctcgaact
    15181 tgcgcaggat gagataacgg gacgcagccc aggtaccgaa tacgaccagg gcggcagcta
    15241 gatagactgc cgcagcttcg cgcccaaacg aacgtgtgta ttgagccgcc atgtgttttg
    15301 actgatatac gacttattcc agcaacttgc tactggaagt tcggcaatga ctcccgtcgg
    15361 gtgatcctca cccctgtcat agggaatcgg ccataaggga cggcccttta tgcgtcgggg
    15421 atcacaattc ccggacggtc atttcggcgt ttgtagtaga ggcctgtatg cccgatgcgt
    15481 tgtaacagga cgctctcggt agtcgcggca agttcggcga gcccggcgtc acgggcggca
    15541 cgctccgcgc cccggaactt caccttgatg agttcatggt catgaagggc gcgttttgct
    15601 tccgcgatga cggccggagt gaccccggcg ttgccgatga gcagcacggg attcaatgca
    15661 tggcccaatc cccgaaggta cttgcgctga cgttctgaaa gttccatcgc cgcattgtaa
    15721 agattgccga tgcctcaggt cttgaaacca aacctcaagg tcctcatcaa aacgttccag
    15781 ggtgtacatc ggagaactca tagaatcctt gtccctggag ttccgggccc atccttgagg
    15841 ctgcggatct gagccttgcc tgctggcgag cggttttcga ttggtgtagc gtgacctcac
    15901 tccttaatc
//
LOCUS       DASQJS010000302         5340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3956329, whole genome shotgun sequence.
ACCESSION   DASQJS010000302 DASQJS010000000
VERSION     DASQJS010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3956329"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VEW08_12130"
     CDS             complement(<1..783)
                     /locus_tag="VEW08_12130"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HYJ41533.1"
                     /translation="MLATRHGFRTMLASTLCAAFVIQSASAAELLDRAVLPANTFSPG
                     PTSGQYASGANGNVLPLINKQPVQGFSAVLRGPVKGTYLVMSDNGFGSKANSPDALLR
                     VDAVKPNFETGEVKPVNRFTGEELSEFTSESFITLRDPWKKIPWPIAADGVNYPGTPT
                     GGGAPIAVDASIKAGRLLTGADFDIEAFRRAPDGTFWFGDEFGPFLIHTDSFGRVLEA
                     PIPLPNFRGFTSTAGGAEVNPLVQAVSNPIRTLAANLPDSGGF"
     gene            complement(940..1956)
                     /locus_tag="VEW08_12135"
     CDS             complement(940..1956)
                     /locus_tag="VEW08_12135"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HYJ41534.1"
                     /translation="MKKKLTIPLVLACGLSLAISQAQTPDEPLEEIIVTGEFAGPGML
                     QVTHPDHPGHTLWIVGEPPLLPQGMRFHSQQVANVASQSQEIILQVGVSLKPDEKVGM
                     FRMLTLVPAAMKLRKNPDKATLQELLPPDVYARWLVQKKLYLGRDSGVEKMRPFLVAE
                     KLRDAAIKELQLGGGGAWGTIAQLVEKKKIPVTSPNLEFTFKTDDLRGQIKAASREKL
                     GDEECFARTVELIEALSNKQVEDARARAWATGDLAKLQELPPLPNPDVACEQAIVNAL
                     AMTTAVPTNLSEQMDELWLAAAGSALTKNESTLALVPMSDLLGEQGYLESLRKRGYEI
                     LAPG"
     gene            2140..3417
                     /locus_tag="VEW08_12140"
     CDS             2140..3417
                     /locus_tag="VEW08_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HYJ41535.1"
                     /translation="MRPIHELATDICLLAGRINAANHRWLMLIAEFDRRNGWLDSSTQ
                     SCAHWLNWKCGIAMGAAREKVRVARALENLPKISAAMESGGLSYSKVREITRVAKAET
                     EDYLLMIAEHGTAQHVEKFVRAYRRCQEAEELSREQRQQQNRRVSFRHDDDGSLVLDC
                     RLPAEAGARIMKALDIAVGELPPKEPVRFSARRADALALMAESFLAHGAIEAAGAERQ
                     QIVVHVAQETLRDRKAGCCEFEDGPSMAAETARRLACDASVVMLTENEDGEPLNVGRK
                     TRTISAPLRRALNSRDKGCRFPGCANTRYMDAHHIDHWANGGETKPSNLVSLCRFHHR
                     AVHEGGIRIERLDDGALRFVKANGVPVDCVAHGFTQPRGDWREMPQGVLVSRWRGERM
                     DLGMAIDGMIHRARQAKDVPAGTSPAPNSPSSR"
     gene            complement(3345..4280)
                     /locus_tag="VEW08_12145"
     CDS             complement(3345..4280)
                     /locus_tag="VEW08_12145"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ41536.1"
                     /translation="MNASCKKSAPTMTSEGMRYFRLRPDARLRPWIKCYWWVEPDPSS
                     ECASAASPDLLIPDGHSELVFRFAGEFSRWQLGESSRTLMNKSYVLGGRSKSVLTQSP
                     GGLRLAGVKLDPRALRALLRMPLSDLRDNTMDFADLGCPALLELEDEIANLRSVDELP
                     GVFDRFFLRRLTGEVRDDSAIELLIERIRATRGAQPILKWAREHGLDARTLERHFVAR
                     MGMTPKQFARVERFKLSYHEMREHQSGERRVHLDGFYDASHFDREFRHFLGTSPISWL
                     NRTPDFTTMIGDHLLEGEFGAGDVPAGTSFACLAR"
     gene            4305..4754
                     /locus_tag="VEW08_12150"
     CDS             4305..4754
                     /locus_tag="VEW08_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015067391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYJ41537.1"
                     /translation="MKTELSINGETRPVEADPDTPLLWVLRDHLGMTGTKFGCGIGQC
                     GACTVHIDGAATRSCLLPLQAAVGAKIATIEGLSPDSGHPLQRAWLDINVAQCGYCQA
                     GMLMAAAALLREHPNPTDEQIDAAMTNICRCGTYHRIRAAIHKAAAG"
     gene            4751..>5340
                     /locus_tag="VEW08_12155"
     CDS             4751..>5340
                     /locus_tag="VEW08_12155"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYJ41538.1"
                     /translation="MNRREFVVATGVIGGGMALALVPRAADASGAASTSELTPWIVIG
                     ADNIVTVRVPGPESGTGNSTQTAMFVAEELSCDWNSVRVEPISFSRNAREGNLYLGVT
                     GIWSTFAGAGASGDLMKALMQAGGSARERLRAAAAAQWKVPVKEIEAKAGVLSHARST
                     RKADYGELAARAATIKLPQEPAPKPRAQWTLLTKQT"
BASE COUNT          991 a         1671 c         1709 g          969 t
ORIGIN      
        1 gaagccgccc gagtccggca ggttcgccgc cagggtgcgg atcgggttgc tgacggcctg
       61 caccagtggg ttcacttccg cgccgcccgc cgtgctggtg aagccgcgga agttaggcag
      121 cgggatcggc gcttcgagca cccgaccaaa cgaatccgtg tggatcagga acgggccgaa
      181 ctcatcgccg aaccagaacg tgccgtccgg cgcgcggcgg aaggcttcga tgtcgaagtc
      241 cgcgcccgtg agcaggcggc cggctttgat cgacgcatcg acggcaatgg gcgcaccacc
      301 acccgtcggc gtgcccgggt agttgacgcc atcggccgcg atgggccacg gaattttctt
      361 ccagggatcg cgcagcgtga tgaagctttc cgacgtgaac tcgctgagct cctcgcccgt
      421 gaagcgattc accggcttca cttcgccggt ttcgaagttg ggcttcaccg cgtccacgcg
      481 cagcagcgcg tcgggcgagt tcgctttcga gccgaagccg ttgtcggaca tcacgaggta
      541 cgtgcccttc accgggccgc gcagcaccgc cgagaagccc tggacgggct gcttattaat
      601 gagtggcagc acattaccgt tggcgcccga ggcgtattgg ccggaggtgg ggcctggcga
      661 gaaagtattg gcgggaagca ccgcgcgatc gagcagctcg gcggcggatg cggattgaat
      721 gacgaaggcg gcacagaggg tcgacgcgag catggtgcgg aaaccatggc gggttgcaag
      781 catgttttta ctccagcgcg cgctgacggc cgaaggatcg ggcgtcgtaa gcaagcggcg
      841 cgatggaatg ttggagtcac cacatttctg gggcgtgaag aatcagcgac acacggtgat
      901 gcgcaccttc tgatcctgga accgaagggc ttttcgatgc tagccaggcg ccaggatttc
      961 gtagccgcgc ttgcgcagac tttccagata gccctgctcc ccgagcagat cgctcatcgg
     1021 cacgagggca agcgtgctct cattcttcgt caacgcgctc cccgccgcag caagccacaa
     1081 ttcgtccatc tgctcgctca gattggtggg gaccgcggtc gtcatcgcca gcgcattcac
     1141 gatggcctgt tcgcatgcga cgtctggatt cggaagcggc ggcaattcct gcagcttggc
     1201 gagatcgccc gtggcccatg cgcgggcgcg ggcgtcttcc acttgtttgt ttgacagggc
     1261 ctcgatgagt tcgactgtcc gtgcgaaaca ctcctcgtcg ccgagcttct cgcgcgaggc
     1321 cgccttgatc tggccacgca gatcgtccgt cttgaaagtg aactcgaggt tggggctcgt
     1381 aacgggaatc ttcttctttt ccacgagctg agcgatcgtt ccccaagccc cgccaccgcc
     1441 gagctgcaat tccttgatcg ccgcatcgcg cagtttttcg gccaccagga agggccgcat
     1501 tttctccaca cccgagtcgc gaccgaggta gagcttcttt tgcaccagcc agcgcgcgta
     1561 aacgtcgggc ggcagcagtt cctgcagcgt ggctttatcg ggattcttgc gcaacttcat
     1621 cgccgccggc accagggtga gcatgcggaa catgccgacc ttttcgtccg gtttcagcga
     1681 gacccccacc tgcaggataa tttcctgcga ttgcgacgcc acgttcgcca cctgctgcga
     1741 atgaaagcgc atcccctggg gcagcagcgg cggttcaccg actatccaga gtgtatggcc
     1801 cggatggtca ggatgcgtga cctgcaacat gccgggtccc gcgaattcac ccgtgacgat
     1861 gatttcttcg agcggctcgt cgggagtctg ggcttgggaa atagctagac tcagtccgca
     1921 ggcgagcacc agcgggattg tcagtttttt cttcacgcgc agcgttggag ggtgcgcgtt
     1981 gtaaatagtt ccggatgaga acgcgcgcct aacaaccttc gaatctttgc tccgcgaatt
     2041 ctcgtgaaga aggcatattt tccgcagcga tttcacgctg cgaagcgtat actgtacgca
     2101 tacacagtcc cgaccccact ctcttatatg gagcggcaca tgcgtccaat ccacgagctc
     2161 gcaaccgaca tctgcctgct ggccggccgc atcaatgcgg cgaatcatcg ctggttgatg
     2221 ttgatcgccg aattcgatcg ccgcaatggc tggctggaca gctccacgca atcctgtgcg
     2281 cattggctga attggaagtg cggcatcgcg atgggagccg cgcgcgagaa agtgcgcgtc
     2341 gcgcgcgcgc tcgagaattt gccgaagatc tccgcagcca tggagagcgg aggtttgagc
     2401 tactccaagg tgcgggagat cacgcgggtt gcgaaagcag agactgagga ctacctgctg
     2461 atgatcgccg agcacggcac cgcgcagcac gtcgagaagt tcgtgcgcgc gtatcgccgc
     2521 tgccaggagg cggaggagtt gtcgcgcgag caacgacaac agcagaaccg ccgcgtgagc
     2581 ttccggcatg acgacgacgg ctcgctcgtc ctcgattgcc ggttgccggc cgaagccggc
     2641 gcacggatca tgaaggcgct cgacatcgcg gtcggggaat tgccgcctaa ggaacccgtg
     2701 cgcttcagcg cgcgccgcgc tgatgccctc gccttgatgg ccgaaagctt ccttgcgcat
     2761 ggcgcgatcg aagctgcggg cgccgagcgg cagcagatcg tggtccatgt cgcgcaggaa
     2821 acactgcgcg atcgcaaagc gggctgctgc gagttcgagg atgggccatc gatggcggcg
     2881 gagaccgcgc gtcgcctcgc ctgcgacgcg agcgtcgtca tgctgaccga aaacgaagac
     2941 ggcgaaccgc tcaacgtcgg ccgcaagaca cggaccattt cggcgccgct gcggcgcgcg
     3001 ctcaactcac gtgacaaggg ctgccgtttt cccggctgcg ctaacacccg ttacatggat
     3061 gcgcatcaca tcgatcactg ggcgaatggc ggtgagacca agccgtccaa ccttgtttcg
     3121 ctgtgccggt ttcatcatcg tgccgtgcat gagggcggca tccgcatcga aagactcgat
     3181 gacggggcac tgcggttcgt gaaggccaat ggcgtgccgg tggattgcgt tgcgcacggg
     3241 ttcactcagc ctcgtgggga ttggagggag atgccgcagg gcgtgctcgt ctcacgatgg
     3301 cgcggggagc ggatggacct tggcatggcg atcgacggga tgattcaccg cgcgagacag
     3361 gcgaaagacg ttccagcggg aacgtcgccg gcgccaaact ccccttccag cagatgatcg
     3421 ccgatcatcg tggtgaagtc cggcgtccga ttaagccagg atatcggtga cgtgcccagg
     3481 aagtgcctga attcgcgatc gaagtgtgat gcgtcgtaga aaccgtcgag atgcacccgc
     3541 ctctcgcccg attgatgctc tctcatttcg tggtagctga gcttgaaacg ctcgacgcgt
     3601 gcgaattgtt tgggcgtcat gcccatgcgc gccacgaagt gtcgttcgag cgtgcgggca
     3661 tcgaggccgt gctcgcgcgc ccacttcagg atgggttgcg cgccacgcgt ggcgcgaatc
     3721 ctctcgatca gtagttcgat cgccgaatcg tcccggactt cgcccgtcaa ccttcgcaga
     3781 aagaatcggt cgaacacccc tggcaactca tcgacggacc tgaggtttgc gatttcatcc
     3841 tcgagctcca ggagcgcggg gcacccgaga tccgcgaagt ccatagtgtt gtctcgaaga
     3901 tcactcagcg gcatgcgcag cagcgcccgc aacgcgcgcg gatccagttt tacgcccgcc
     3961 aaccgaagtc cgcccgggct ttgtgtgagc accgacttcg agcggccgcc gagcacatag
     4021 ctcttgttca tgagcgttct ggaggactcg ccgagttgcc agcgtgagaa ttctccggcg
     4081 aaccggaata ccaattccga atgcccatcg ggaatcagca gatctggtga agccgcagac
     4141 gcgcactcgc tgctcggatc cggctccacc caccagtaac atttgatcca cggcctcaag
     4201 cgcgcatcgg gcctcagccg aaagtagcgc atgccttcgc tggtcatggt cggtgccgat
     4261 tttttacaag acgcgttcat cgcctcgttc aatactgatt ccgcatgaaa accgaactca
     4321 gtatcaacgg cgagacgcgc cccgtcgagg ccgaccccga cactcctctt ctatgggtgc
     4381 tgcgcgacca cctggggatg acgggcacga agttcggatg tggcatcggc cagtgcggcg
     4441 cctgcacggt gcacatcgac ggcgccgcca cgcgttcgtg cctgttgccg ctgcaggccg
     4501 cggtgggcgc gaagatcgcg accatcgaag gactgtcccc ggattcgggc catccgttgc
     4561 agcgcgcctg gctggacatc aacgtcgccc agtgcggcta ctgccaggcc ggcatgctga
     4621 tggcggccgc cgccctgctg cgggagcacc cgaatcccac cgatgaacag atcgatgcgg
     4681 cgatgaccaa catctgccgc tgcggcactt accaccgtat ccgcgctgcg attcacaagg
     4741 ccgcagccgg atgaaccgtc gagagttcgt agtggccaca ggcgtcatcg gcggcgggat
     4801 ggcgctcgcc ctcgtgccgc gcgccgccga tgcgtctggg gccgcgtcca cttccgaact
     4861 gacgccatgg atcgtcatcg gcgcggacaa catcgtgacc gtgcgcgtac caggcccgga
     4921 atccggcact ggcaattcca cgcagacggc catgttcgtg gccgaagaac tgagctgcga
     4981 ctggaacagc gtgcgcgtcg agcccatctc gttcagccgc aacgcgcgcg aaggcaattt
     5041 gtacctcggc gtcaccggca tctggtccac gttcgcgggc gccggcgcat cgggagacct
     5101 catgaaggcg ctgatgcagg ccggagggag cgcgcgcgaa cggctgcgcg cagccgcggc
     5161 ggcgcagtgg aaagtgcccg tcaaggaaat cgaggcgaaa gccggcgtgc tgtcgcacgc
     5221 gcgctcgacg cgcaaggcgg actacggcga actcgcggcc cgtgccgcga cgatcaaact
     5281 accgcaggag ccggctccga aaccgcgcgc gcagtggacg ctgctcacga agcagacatt
//
LOCUS       DASQJS010000303        13451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2695816, whole genome shotgun sequence.
ACCESSION   DASQJS010000303 DASQJS010000000
VERSION     DASQJS010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13451
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2695816"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..223)
                     /locus_tag="VEW08_12160"
                     /pseudo
     CDS             complement(101..223)
                     /locus_tag="VEW08_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290913.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
     gene            complement(257..1480)
                     /gene="bamB"
                     /locus_tag="VEW08_12165"
     CDS             complement(257..1480)
                     /gene="bamB"
                     /locus_tag="VEW08_12165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HYJ41539.1"
                     /translation="MKQLSLVLLAALFALSACDSKNAKVDKPAELTNLKNPTVRIQKV
                     WGASVGGGGKKLRLGLGLATAGNKLFAAGREGDVAAFDLKTGRQLWRTETKLELAGGT
                     GVGADAVAVGSADGQVVVLAMDTGAQRWRADVKGEILSAPAVAENEVIVRTVDGKLRA
                     LALADGKEIWTAEQQIPRLTLRGAAAPVVTRDIAITGFDNGRVLAVSLADGATVWDSP
                     VSPPHGRTELERLNDIDAPVKVLGEEVFVAGYQGRAAMLALDSGQAWWTSDVSSYRGV
                     DVDDERMYVSNSQGELLAITRRTGQPVWATAVLKLRSLSAPVVAGEYVVVADLEGYVH
                     YFDRATGELAGRTKSGGDRVTNAPLAVGDVVYVVTDKGDIFALRGTPVASQAAKTETP
                     PAPVPAPAAAPAPGG"
     gene            complement(1480..2133)
                     /locus_tag="VEW08_12170"
     CDS             complement(1480..2133)
                     /locus_tag="VEW08_12170"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ41540.1"
                     /translation="MSDYSEAEQMERAKSWLKANGIWIIAGIAIGVGGWGAWHWYQDR
                     RNAQAETASARYEELVDAFTRNDKARGMTLLDQLNREYGWTPYSSLGTLIAARVQVEA
                     NELDKAAASLKSVMDNAKDDELKMVARMRLARVQAAQAKYDDALATLKVENAGEFGPR
                     IADTRGDVLLAKGDRAGALKEYLAARSTEDNGRIDFDLLDLKIRDLGGTPPATPGES"
     gene            complement(2130..3413)
                     /gene="hisS"
                     /locus_tag="VEW08_12175"
     CDS             complement(2130..3413)
                     /gene="hisS"
                     /locus_tag="VEW08_12175"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HYJ41541.1"
                     /translation="MSRNIPPVTGMKDVLAADIGLWQHVERSARAVMDAYGYEEIRVP
                     VVEHTELFKRAIGEYTDVVEKEMYTFTDQGGDSLTLRPEATAGIVRATISNGLLRGAR
                     LKLWCQGPMFRHEKPQKGRFRQFNQVDLEAIGFAGPDVDIEIIALTARLWKLLGLTRV
                     KLQINSLGTPESRKAYRTLLQEYFRAHLDALDEDGKRRLEGNPLRILDSKHPPTQAIV
                     GNAPVLTDHLDAESKEHFAQLRAGLDSLGIPYTVNPRLVRGLDYYSRTVFEWITDALG
                     SQDAICSGGRYDGLIAQMGGESTPAIGWAMGVERVVELVRLAGQTPPSRAPDVFVMAQ
                     GDAAQRAALGLAESLRDALPGRGVVVHCGSAKFKTQFRRADESGARLALIVGDDELAR
                     GVIALKPLRGAEGTQTEHPVADLTARVEEFLRRST"
     gene            complement(3437..4333)
                     /locus_tag="VEW08_12180"
     CDS             complement(3437..4333)
                     /locus_tag="VEW08_12180"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HYJ41542.1"
                     /translation="MDETVAQEAPAKPRSIGERLRAGRERSGLSVAAAAEKLHLDVKV
                     IEALEADRFAELGASVYVRGHLRRYADFVGEPGVELVNSYSARDARPPPPDLTQVPHA
                     ERRADPRRLVTPLVGLGCAAVLLVAIWWVLAGSRSGNADGASTLPTPVVQPGDPANTA
                     SGAATPVALSSSTPAIPANKVPAAAAPGADSTTTQAKRDAASDTSQAPVRETQLRLDL
                     NNDSWIEVYDSRDRKLFYDVASAGSVQTVSGRGPLRVVLGNAAGVTVEVDGQAREIPA
                     GAIDGEGASFVVNRSGSLARAR"
     gene            complement(4339..5094)
                     /gene="pilW"
                     /locus_tag="VEW08_12185"
     CDS             complement(4339..5094)
                     /gene="pilW"
                     /locus_tag="VEW08_12185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HYJ41543.1"
                     /translation="MNIRWIAVLSVCLLGACVSSDGTKRLKREDPTEAAAKTNVQLGV
                     AYLQQGNYSLAREKLERSLKQNPKDPDVHTSLGLLYDRVGEPKLADKHFREALKLAPT
                     NPDISNNYAIYLCKNGRVEEGVERFTAVAVNKYYRTPEVALSNAGVCLRGAKRLDEAQ
                     AMFVKSIKARPNYNEATVQLATLHVERGQLVEARKVVDTYLGAFRPDPEVLFAAVTVA
                     RAAKDKMSEEKFSRTLRLEFPESAQARALKRGS"
     gene            complement(5091..6218)
                     /gene="rlmN"
                     /locus_tag="VEW08_12190"
     CDS             complement(5091..6218)
                     /gene="rlmN"
                     /locus_tag="VEW08_12190"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HYJ41544.1"
                     /translation="MSAAAPSVRTNLLGLPKAELESFVAEMGSKPFRARQLMNWMYKR
                     GEGDIAAMTDLAKDFRAELVQRAEVAVPEIVKTQVASDGTRKWLLSGGNGQAFEMVYI
                     PEEDRGTLCISSQVGCALDCSFCSTAQQGFNRNLTTAEIVGQVWLAHKILGGESGWQV
                     GDNRIITNIVFMGMGEPLANFRNVVPAIQIFLDDLGFDISRRRVTLSTSGLVPQIYKL
                     AEEVNCALAVSLHAPNDELRNQLVPINRKHNIAELLQASWHYLDEQNGRSVTFEYVML
                     DGVNDQPEHARELAKLLRDKPAKLNLIPFNPFPGTTYRRSPAAAIEKFRDELMQRGLI
                     VTIRRTRGDDIDAACGQLAGQVTDRTIVRLGSKTFGLQVRA"
     gene            complement(6229..6657)
                     /gene="ndk"
                     /locus_tag="VEW08_12195"
     CDS             complement(6229..6657)
                     /gene="ndk"
                     /locus_tag="VEW08_12195"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HYJ41545.1"
                     /translation="MALERTLSIVKPDGVARNLIGQVIGRFEKVGLKVVAARMVHLSQ
                     RDAEGFYAVHAARPFFKDLVKYMTSGPVLVQVLEGEGAIAKNREVMGATDPKKAAPGT
                     IRADLAESIEANTVHGSDSAENAAIEIAYFFRTTELCPRG"
     gene            complement(6815..7138)
                     /gene="iscA"
                     /locus_tag="VEW08_12200"
     CDS             complement(6815..7138)
                     /gene="iscA"
                     /locus_tag="VEW08_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016537756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HYJ41546.1"
                     /translation="MSISLTPAAADRVRTFIASRGKGLGLRLGVRKTGCSGFAYVVNY
                     ADDAQAHDLVFEDQGVKVFVDPESLPLINGTTVDFVKQGLNEAFRFHNPNIKGECGCG
                     ESFNV"
     gene            complement(7161..8759)
                     /locus_tag="VEW08_12205"
     CDS             complement(7161..8759)
                     /locus_tag="VEW08_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYJ41547.1"
                     /translation="MTIYLDYAATTPVDPRVAAVMAECLADPHAQANPASVTHGPGLM
                     ARQRVEAARAEVAALIGASPGEIIFTSGATEANNLGLLGVARAELRARAGRSHIVSSR
                     TEHKSVLDTCKQLEREGFAVTLVEPDPSGRVTVETVRAALRADTLLLSLMWVNNEIGT
                     ISDIAAIAKICRASGILVHTDASQAVGKLPLDVNLLGVDFLSLTAHKFYGPKGIGALY
                     IRESARPRIAPIQFGGGHERGLRSGTLPTHQIVGLGMAATLARAEGTGDAAHARDLVS
                     RLWQELEPVAGVMLNGHREQRVPGLLNVSFPGVEGESLITGLADFALSSGSACSSATR
                     EPSYVLRSLGRSTELAQSSLRLSLGRFTTAAEVDTAATAIRSEVARLRTLAGTLEPGF
                     EPAVTRGPDTGGPAESQLAQFLNPLARRYFRAAPRTPAFPEGNLPPGVRQGRAGKQAD
                     GTAVCFELQIADGIVKSARFSVYGCPHTVAVVAWLCDELQGFRLDAGTLGTPADWAQA
                     FDVPAEKLGRLLIVEDALRAALYR"
     gene            complement(8761..9516)
                     /locus_tag="VEW08_12210"
     CDS             complement(8761..9516)
                     /locus_tag="VEW08_12210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HYJ41548.1"
                     /translation="MIQAVPLTAIRIVLVEPSHPGNIGAVARAMKNMALAELVLVRPK
                     AFPHPEATARASGADDLLGRARVVGSVVEAVADCGFVAATTSRPRDQNFRALDVHEAG
                     ARIFEMSERGPAAVLFGAERTGLTNEELALAHLLIRIPANPEYPSLNLAMAVQLVCYE
                     LFRAAGSPASERPLSESPVPLAPGEDMERFYAHLAEVMEEVDFRDRTQSGTQLMTRIR
                     RLFQRCEMDQNEVNILRGLLTSVQNKRRPAGKA"
     gene            9546..10346
                     /locus_tag="VEW08_12215"
     CDS             9546..10346
                     /locus_tag="VEW08_12215"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HYJ41549.1"
                     /translation="MQPLLSIAVKAARRAGDIIVRAIPRLEAVQVESKGRNDFVTEVD
                     KAAEADIIQTIRRLYPDHAILAEESGASGDNEVVWIIDPLDGTTNFMHGFPTFAVSIA
                     CQIRGRLEHAVVFDPMRQEVFTASRGDGAQCEGRKIRVSKQITLEGSLIATGFPFRDD
                     APYLNDYLAMLKAVMIKAQGVRRAGSAALDLAYVAAGRVDGFWEIGLKPWDTAAGTLL
                     ITEAGGRIGTIFGEEYKQGGHVIAGTAKVYAALVAELSPHAPASLREA"
     gene            complement(10343..12160)
                     /locus_tag="VEW08_12220"
     CDS             complement(10343..12160)
                     /locus_tag="VEW08_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYJ41550.1"
                     /translation="MGWRTKSIDQVQKDFDRGELKRTLGPIHLVLFGIGCIIGAGIFV
                     RTGTAAAQYAGPAVMLSFVLAGVVCAFAGLCYAELASMLPVSGSAYTYAYTTVGEFGA
                     WIMGALLLLEYALAASVVAVGWSGYAVSLLHDIGWNLPAALTQPYGELVVAALSTITV
                     VDPSVSLNGVTAALADGSAVQFVTEAKAAIGGSYTAAIAQYDVVKAAENGFTLINALD
                     IPISTDINATLAAATKVLDPATGNLRDLAAGTLLNVPASSVVHLPAATVVSIPAAAVV
                     TSMFNMPAVFIVLAVTALLVYGVSESATVNNIIVAIKVTVILAFIAVGAFFVNTDNWH
                     PFIPEPTGVEDQFGIDGIIRAAMIVFFAYIGFEAVSTAGQEARNPRKDMPIGILGSLV
                     ICTILYMATAAVLTGVISFTKLNVAAPVATAVDSFGPEWGWLAKCIKIGAIAGLSSVV
                     LVLMFGQTRVFYSMSRDGLMPKVLAKVHPKYKTPWINTLIVGVLVAFAAAVFDINALG
                     DLTSVGTLFAFGLVCFSVIWLRYKRPDLPRPFKVWGYPVVPAIGVVICFWLAYDGATI
                     KIRWWFFWFLLAAIGLYFVYAYWKSPLRPKNQAEAKPAG"
     gene            complement(12246..12995)
                     /locus_tag="VEW08_12225"
     CDS             complement(12246..12995)
                     /locus_tag="VEW08_12225"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HYJ41551.1"
                     /translation="MELLADPHAWIAFITLSALEIVLGIDNIIFISILVSRLPPERRE
                     AARITGLALAMLTRIALLFSIVWLTRLIKPWFSILGQEFSGRDIILLLGGLFLLAKSV
                     TEIHGTLEGAEDDRKARVFANFTVIVVQIAIIDIVFSLDSVFTAVGLAKPDQLPIMVA
                     AIVLAIGVMMWVSGSIGAFIERHPTIKILALAFLILVGVVLVADSFHFDVPKGYLYFA
                     MAFSVGVEMVNIRLRRHMDERRNKKDETQGG"
     gene            complement(13017..>13451)
                     /gene="secF"
                     /locus_tag="VEW08_12230"
     CDS             complement(13017..>13451)
                     /gene="secF"
                     /locus_tag="VEW08_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HYJ41552.1"
                     /translation="AVMHDPILVLGVFSVTQTLFDLSVVAAILAVIGYSLNDTVIVFD
                     RIRERFEHNRRQASDVTLDQAINQTLSRTIMTKLVTSIVVVALLLLGGSALRGFSEAL
                     LIGIVAGTYSSIYISSAIALSCGLKAEHLFPTVRKDPIDELP"
BASE COUNT         2363 a         4373 c         4369 g         2346 t
ORIGIN      
        1 cggtctggat gcgcatgagc gcgtccatgc cgatcgggtt ttcgaccagc ccgccggtgt
       61 cgatgcagac gtactgcacc ggacccagcc ttccgtagcc atattgacga tcgcgcgtga
      121 caccgggcac gtcggcgacg atggcgtcac gcgttttcgt cagcgtgttg aacagcgtgg
      181 acttgccgac gttcggccgt ccgacgatcg cgatgaccgg cagcatggga gcggggttgc
      241 ggaactgagc gcgggctcag ccgcctggcg cgggtgcagc tgcaggtgcg ggtacgggcg
      301 caggcggtgt ttccgtcttc gccgcctggc tcgccaccgg cgttccacgc aacgcgaaga
      361 tgtcgccctt gtccgtgacg acgtaaacga cgtctccaac cgccagcggc gcattcgtga
      421 cacggtcacc gcccgacttg gtacgcccgg caagttcgcc ggtcgcccgg tcgaaatagt
      481 gcacgtagcc ttcgagatcg gcgaccacca cgtactcgcc cgccaccacg ggcgccgaga
      541 ggctgcgcag cttgagcacc gcggtggccc acacaggctg gcccgtgcgg cgcgtgatcg
      601 ccagcagctc gccctgcgag ttggacacgt acatgcgctc gtcgtcgacg tcgacgccgc
      661 gatagctgga cacgtcgctc gtccaccagg cctgacccga atcgagcgcc aacatggcgg
      721 cacgaccctg gtaacccgcg acgaacactt cttcgcccaa caccttcacg ggagcgtcga
      781 tgtcgttgag acgttcgagt tccgtgcggc catgcggcgg cgaaaccggt gaatcccaga
      841 ccgtcgcgcc atccgccagg ctgacggcga gcacacggcc gttgtcgaac ccggtgatcg
      901 cgatatcccg tgtcaccacg ggcgcagcgg cgccacgcaa cgtgaggcgc gggatctgct
      961 gttccgcggt ccagatttcc ttgccatcgg cgagcgcgag cgcgcgaagc ttgccgtcga
     1021 cggtgcgcac gatgacttcg ttctcggcca cggcgggggc cgacaggatt tcgcccttca
     1081 cgtcggcgcg ccagcgctgc gcgccggtgt ccatcgccag gacgacgacc tggccatcgg
     1141 cagaacccac ggcgacggcg tcggcgccaa cgccggtgcc accggccagt tccagtttgg
     1201 tctcggtgcg ccagagctgt ctgccggttt tcagatcgaa ggcagcgaca tcaccttcac
     1261 ggccggccgc gaagagcttg ttgccggcgg tggcgaggcc caacccgagg cgcagcttct
     1321 tgccgccgcc acccacgctt gcgccccaga ccttctggat gcggaccgtg ggattcttga
     1381 ggttggtgag ctccgcgggc ttgtcgacct tcgcgttctt cgagtcgcac gcgctcaagg
     1441 cgaacaacgc cgcgagcaga accaggctca gctgcttcat caggactccc cgggagtagc
     1501 gggcggcgtg ccgccaaggt cgcggatctt aaggtcgagc agatcgaaat cgatgcggcc
     1561 gttgtcttcc gtcgatcgtg ccgcgagata ttccttcagc gcgccggcgc gatctccctt
     1621 ggcgagcaga acgtcgcccc gggtatcggc gatgcgcggc ccgaactcac cggcgttttc
     1681 caccttgagc gtggcgagcg catcgtcgta tttggcttga gccgcctgca cgcgcgccag
     1741 gcgcatccgg gcgaccatct tgagttcatc gtctttggca ttgtccatta cggacttgag
     1801 actcgcggcg gccttgtcga gttcattcgc ttcgacctga actcgcgccg cgatcagcgt
     1861 cccgagcgag gagtacggcg tccagccata ttcgcggttg agctgatcga gcagcgtcat
     1921 gccgcgggcc ttgtcgttgc gcgtgaacgc atcgaccagt tcctcatacc gcgccgaagc
     1981 cgtctcggcc tgggcgttgc ggcgatcctg gtaccagtgc caggctcccc agcctccgac
     2041 gccgatcgcg atgcccgcga tgatccagat tccgttcgct ttgagccagc tcttggcccg
     2101 ttccatttgt tctgcttcgc tgtagtcgct cacgtcgatc tcctcaaaaa ctcttcgacc
     2161 cgcgcggtga gatccgcgac ggggtgttcg gtctgggtgc cttcggctcc acgcaacggc
     2221 ttgagcgcga tcacgccgcg cgccagttcg tcgtccccca cgatgagtgc cagccgcgcc
     2281 cccgattcgt cggcgcggcg gaactgtgtc ttgaacttgg ccgatccgca atggaccacg
     2341 acgccgcgcc ccggcaaagc gtcgcgcagg ctttccgcga ggcctaacgc ggcacgctgg
     2401 gcggcatcac cctgggccat gacgaagacg tccggtgccc gggacggcgg cgtctgcccg
     2461 gcaagccgga ccaactccac cacgcgctcc acgcccatgg cccagccgat ggccggagtg
     2521 gattcaccgc ccatctgcgc gatcagtccg tcataccgac cgccggagca gatagcatcc
     2581 tggctgccga gagcgtcggt gatccactcg aatacggtgc gcgagtagta atcgaggccc
     2641 cgcacgaggc gcggattcac cgtgtaggga atccccaggc tgtcgaggcc cgcgcgcaac
     2701 tgcgcgaaat gttccttcga ttcggcatcg aggtgatccg tgagcaccgg tgcgttaccg
     2761 acgatggcct gcgtcggcgg atgtttgctg tcgaggatgc gcagcggatt gccttcgagg
     2821 cggcgcttgc cgtcctcgtc gagggcgtcg aggtgcgcgc ggaagtattc ctgaagcaac
     2881 gtgcggtacg ccttgcgcga ctccggcgtg cccagagagt tgatctgcag cttcacgcgc
     2941 gtgagaccta acaacttcca caggcgcgcc gtcaacgcga tgatttcgat gtcgacgtcg
     3001 ggacccgcga agccgatggc ctcgaggtcg acctggttga actggcggaa ccggcccttc
     3061 tgcggcttct cgtgccggaa catcgggccc tggcaccaca gtttcaggcg cgcgccacgc
     3121 agcagaccgt tggagatggt cgcgcgcacg atgccggccg tggcctcggg acgcagcgtg
     3181 aggctgtcgc caccctggtc ggtgaaggtg tacatctcct tctcgaccac gtccgtgtat
     3241 tcgccgatgg cgcgcttgaa cagttcggtg tgctcgacca cgggcacgcg gatttcttca
     3301 tatccgtatg cgtccatgac ggcgcgcgca ctgcgttcca cgtgttgcca caaccctata
     3361 tcggcggcaa ggacgtcttt catgccggta accggcggga tattgcggct catggtcttt
     3421 ttttgaggag gcttgtttat ctggcgcgcg cgagcgagcc gctgcgattc acgacgaaac
     3481 ttgcgccttc cccgtcgatg gcaccggctg gaatttcgcg cgcctggccg tcgacttcga
     3541 cggtgacccc ggcggcgttg ccgagcacga cgcgcaaggg accgcggccg ctcacggtct
     3601 gaacactgcc cgcgctcgcc acgtcataga acagcttcct gtcgcgcgaa tcgtagacct
     3661 cgatccagct gtcgttgttc agatcgagcc tcagctgcgt ctctcgcacc ggagcctggc
     3721 tcgtgtccga ggccgcgtcg cgtttcgcct gagtggtggt gctatccgcg cccggcgccg
     3781 ccgcggcagg caccttgttg gccgggattg cgggcgtgct ggacgacagc gccaccggcg
     3841 tcgccgcgcc ggaagccgtg ttcgcgggat caccaggctg caccaccggt gtcggcagcg
     3901 tcgatgcgcc atccgcattg ccggaacgcg agccggcgag aacccaccag atggcgacca
     3961 gcagcacggc cgcacatcca aggccgacca gcggcgtgac cagccgccgc ggatccgcgc
     4021 ggcgctccgc gtgaggaacc tgcgtcaggt ctggcggcgg aggacgcgca tcgcgggccg
     4081 agtaactgtt gacgagctcg acgccgggct cacccacgaa atccgcgtag cggcgcaggt
     4141 gaccgcggac gtacacggat gcgccgagct cggcgaaacg gtccgcttcc agcgcctcga
     4201 tgaccttcac gtccaggtgc agtttttcag ccgccgccgc gaccgacagg ccggagcgtt
     4261 cacggccggc gcgcaggcgc tcgccgatcg aacgcggctt ggccggggct tcttgcgcga
     4321 ccgtctcgtc catcgggctc aactgccgcg cttgagcgca cgcgcctgcg cggattccgg
     4381 aaattccaga cgcagcgtgc gcgagaattt ctcttcgctc atcttgtcct tggcggcgcg
     4441 cgccacggtc accgcggcga acagcacttc cgggtcggga cggaaggcgc cgagataggt
     4501 atcgaccacc ttgcgcgcct cgacgagctg gccgcgctcc acgtgcagcg tcgcgagctg
     4561 caccgtagct tcgttgtagt taggcctggc cttgatggac ttcacgaaca tggcttgcgc
     4621 ctcgtcgagt cgcttggcgc cgcgcagaca gacgccggcg ttgctgagcg caacttccgg
     4681 cgtgcggtag tacttgttga cggccaccgc cgtgaatcgc tccacgccct cttcgacccg
     4741 gccgttcttg cagaggtaga tggcgtagtt gttggagatg tcggggttgg ttggcgcgag
     4801 cttcagcgcc tcgcggaaat gcttgtcggc gagcttgggc tcgcccacgc ggtcgtacag
     4861 cagcccgagg ctggtgtgga cgtcgggatc cttgggattc tgcttgagcg agcgttccag
     4921 cttttccctg gcgagcgagt aattaccctg ctgcaggtag gcaacgccca gctggacatt
     4981 ggtcttggca gccgcctcgg tcggatcttc gcgcttgagc cgcttggtgc catcgctgga
     5041 gacgcaagcc ccgagcaggc agacggagag gacggcaatc catcggatat tcatgctcgc
     5101 acctgcaggc cgaaagtttt tgaccccaac cgcacgatgg tgcggtcggt gacctggccc
     5161 gcgagttgtc cgcaggccgc atcgatgtcg tcgccgcgcg tcctgcggat ggtgacgatg
     5221 agcccgcgct gcatgagttc gtcgcggaat ttttcgatcg ccgcagcggg tgagcggcga
     5281 tacgtcgttc cgggaaacgg attgaatgga atgagattga gcttggcggg tttgtcacgc
     5341 aagagctttg ccaactcgcg cgcgtgttcc ggctggtcgt tcacgccatc tagcatcacg
     5401 tattcgaacg tgacgctgcg gccattctgc tcgtcgaggt aatgccagct ggcctgcagc
     5461 aactcggcga tgttgtgctt gcggttgatg ggcacgagct ggttgcgcaa ttcgtcgttc
     5521 ggcgcgtgca acgagacagc cagcgcgcag ttcacttctt ctgccagctt gtagatctgc
     5581 ggcacgaggc cggaagtgct caacgtgacg cggcggcgcg agatgtcgaa cccgagatcg
     5641 tcgaggaaga tctggatcgc cggcaccacg ttgcggaaat tggccagcgg ctcacccatg
     5701 cccatgaaca cgatgttggt gatgatgcgg ttatcgccca cctgccagcc cgactcaccc
     5761 cctaaaattt tatgggccag ccacacctgg ccgacgatct cggcagtggt gaggttgcgg
     5821 ttgaacccct gctgtgcggt cgaacagaac gagcagtcga gtgcgcaacc cacctgcgag
     5881 gagatgcaca acgtgccgcg atcttcctcc gggatgtaga ccatctcgaa ggcctggcca
     5941 ttgccgcccg acagcagcca cttgcgtgtg ccgtcggagg caacctgcgt cttgacgatt
     6001 tccggcaccg cgacttccgc gcgttgcacg agctcggcgc gaaaatcctt ggcaaggtcg
     6061 gtcatcgccg cgatgtcgcc ctcgccacgc ttgtacatcc agttcatgag ctggcgcgcg
     6121 cggaacggct tgctgcccat ctccgcgacg aacgactcca gctcggcctt cggcaggcct
     6181 aacaagttgg ttcgtacgct gggggctgcg gcgctcatcg gaggcaggct acccgcgcgg
     6241 gcagagctcc gtcgtgcgga agaaatacgc gatctcgatc gcggcgttct cggcggaatc
     6301 cgaaccatgc acggtgttcg cttcgatgct ctcggcgaga tcggcgcgga tcgtgcccgg
     6361 ggccgccttc ttcggatcgg tggcgcccat gacttcgcga ttcttcgcga tggcgccttc
     6421 accttcgagc acctgcacga gcaccggacc cgaggtcatg tacttcacga gatccttgaa
     6481 gaacgggcgc gcggcgtgca cggcatagaa tccttcggcg tcgcgctgcg acagatggac
     6541 catgcgcgcg gcgacaacct tgaggccgac cttttcgaag cggccgatga cctggccgat
     6601 gaggttacgg gcaacgccgt cgggcttgac gatggacaga gtgcgctcga gcgccatgcg
     6661 aatgattcct tgtgggggtc agtaaaggcg cgcgattgta ccggctggca cgctcgcctg
     6721 cgagttaacc cgctgaaatc caagtattac tgcggtacgg acggcgccgt tttccgaagt
     6781 ggcgcgtgag cgcctgggaa gtgcgcctgt gaatctagac gttgaaagac tcgccgcaac
     6841 cacactcgcc cttgatgttc gggttgtgga agcggaaggc ctcattgagg ccctgtttga
     6901 cgaagtcgac cgtggttccg ttgatgagcg gcaggctctc ggggtcgacg aacactttga
     6961 ccccctggtc ttcgaacacc aggtcatggg cctgggcgtc gtcagcgtaa ttgacgacat
     7021 aagcgaagcc cgagcagccg gtcttgcgca cacccagccg cagccccaga cccttgccgc
     7081 gcgaggcgat gaaggttctg acgcgatcgg ccgccgctgg tgtgagtgaa atggacatct
     7141 tttctctttc ctggcgcgcg ctagcgatac aacgcggcgc gcaatgcatc ttcgacgatc
     7201 aggagccggc caagtttctc agccggaaca tcaaatgcct gggcccagtc ggcgggagtt
     7261 cccagggttc cggcatcgag cctgaagccc tgaagctcat cacataacca agcgactacg
     7321 gccacagtat gggggcatcc gtacacgctg aaacgggcac ttttcacaat cccgtcggca
     7381 atttgcagtt caaaacacac cgcggtcccg tcggcctgct ttccggcccg cccctggcgc
     7441 acgccgggag ggagattccc ctccggaaaa gccggagtcc tcggagcggc ccggaaatac
     7501 cggcgcgcca gagggttcag gaactgggcg agctggctct cggcaggccc gccggtgtcg
     7561 ggtccacgag tgacggccgg ctcgaatccc ggctcgagag tacccgccag cgtacgcagg
     7621 cgcgcgacct cggaccggat ggccgtagcg gccgtatcga cttccgcggc cgtcgtgaat
     7681 ctccccagcg acagccgcaa ggagctctgc gcgagctcgg tactgcgccc gagcgaacgc
     7741 aggacgtaac tcggctcccg cgtcgccgag ctgcaggccg agcccgacga cagcgcaaag
     7801 tccgcgagac cggtgatcag gctttcgccc tcgacgcccg gaaacgacac gttgaggagt
     7861 cccggaacac gttgctcccg gtgcccattg agcatgaccc cggccacggg ttcgagctcc
     7921 tgccagagcc gcgacacgag gtcgcgcgcg tgcgccgcgt cgcccgttcc ttccgcacgc
     7981 gcgagcgtcg ccgccatgcc caacccgacg atctggtgcg tgggcagcgt gccggagcgc
     8041 aagccgcgct cgtgtccgcc accgaactgg atcggcgcga ttctcggccg cgcgctttcg
     8101 cgaatgtaca gcgcaccaat gcccttcggg ccgtagaact tgtgcgccgt caggctcagg
     8161 aagtcgacac cgagaaggtt tacatccagc ggcagtttgc ccaccgcctg cgacgcgtcg
     8221 gtatgcacga ggatgccgct cgcgcggcag attttcgcga tcgcggcgat atccgaaatc
     8281 gttccgatct cgttgttgac ccacatcagc gacagcagca gcgtgtcggc gcgcagcgcg
     8341 gcgcgaactg tttcgacggt cacccgcccg ctcgggtccg gttccaccag cgtgactgcg
     8401 aagccttctc tttcgagctg tttgcaggtg tcgagcaccg atttgtgctc ggtgcgcgag
     8461 ctgacgatgt ggctgcggcc ggcccgcgcg cgcagctcgg cccgcgccac gccgagcagg
     8521 cccaggttgt tagcctcggt ggcgccggag gtgaagatga tctcgcccgg ggaagcgccg
     8581 atcagcgccg caacctcggc gcgcgccgcc tcgacgcgct gccgggccat gagtcccgga
     8641 ccgtgggtga cggaggccgg attcgcctgg gcgtgcggat cggcgaggca ttccgccatg
     8701 accgcggcga cccgcggatc caccggcgtg gtggcggcgt agtcgaggta gatagtcacg
     8761 tcacgccttg ccggccgggc gccgcttgtt ctgtaccgag gtcagcaggc cgcgcaggat
     8821 gttgacctcg ttctggtcca tctcgcagcg ctggaacaac ctgcggattc gcgtcatgag
     8881 ttgcgtgcca ctctgggtgc gatcgcggaa atcgacctct tccatgactt cggcgaggtg
     8941 cgcgtaaaaa cgctccatgt cctcgccggg cgcgagcggc accggcgact cggacagggg
     9001 ccgttcgctc gccggactgc cggcggcgcg gaacagctcg taacacacca gctgtacggc
     9061 catggcgagg ttcagcgatg ggtattccgg attcgcgggg atgcggatca gcagatgggc
     9121 gagcgccagc tcttcgttgg tgaggccggt gcgttcggcg ccaaacagca cggcggcagg
     9181 accgcgttcg ctcatctcga agatgcgcgc gccagcctcg tgcacatcga gcgcgcggaa
     9241 gttctggtcg cggggacgtg aggtcgtggc ggccacgaag ccacagtcgg ccaccgcctc
     9301 gacgacgctg cccacgacgc gcgcccggcc aagcagatcg tcggcgcccg aggctctcgc
     9361 cgtggcttcc gggtgtggaa acgccttcgg gcgtaccagc accagctccg ccaacgccat
     9421 gtttttcatg gcgcgcgcca ccgccccgat gtttccggga tgtgagggct cgacgagcac
     9481 gatgcgaatg gcggtcagcg gcacggcctg tatcatagcg acccttttcg caggcccctc
     9541 ccccgatgca gcccctactc agtattgcgg tcaaagcggc gcgccgcgcc ggcgacatca
     9601 tcgtccgcgc cattccgcgc ctcgaagccg tccaggttga aagcaagggc cgcaacgact
     9661 tcgtcaccga ggtcgacaag gcggccgaag ccgacatcat ccagaccatc cgccggctgt
     9721 atccggatca cgccatcctg gccgaggaga gcggcgcatc gggcgacaac gaggtcgtct
     9781 ggatcatcga cccgctcgat ggcaccacaa acttcatgca cggctttccg acctttgccg
     9841 tatccatcgc ctgccagatc cggggacggc tggagcacgc ggtggtcttc gatccgatgc
     9901 gccaggaagt cttcaccgct tcgcgcggcg acggcgccca gtgcgaaggc cgcaagatcc
     9961 gcgtcagcaa gcagatcacg ctggaagggt cgctgatcgc caccggcttt ccgtttcgcg
    10021 acgatgcgcc ctatctcaat gattacctgg ccatgctcaa ggccgtgatg atcaaggcgc
    10081 agggagtgcg ccgcgccggc tcggcggcgc tggacctggc ctatgtggcc gccggtcgtg
    10141 tcgacggctt ctgggagatc ggcctcaagc cctgggacac cgctgcgggc acgctgctca
    10201 tcaccgaagc cggcggccgt atcggcacca tcttcggcga agaatacaaa cagggcggcc
    10261 atgtcatcgc cggcaccgcc aaggtgtacg ccgcgctggt ggcggagctt tcgccccacg
    10321 caccagcgag tcttcgcgaa gcctaaccag ccggcttggc ttcggcctgg ttcttcgggc
    10381 gcaacgggct tttccagtag gcgtagacga agtagagccc gatcgcggcc agcaggaacc
    10441 agaagaacca ccagcgaatc ttgatcgttg cgccgtcgta cgcgagccag aagcaaataa
    10501 ccacgccgat cgccgggacc accggatatc cccacacctt gaaggggcgc ggcagatccg
    10561 ggcgcttgta gcgcagccag atcaccgaga agcacaccag gccgaaggcg aacaacgtgc
    10621 caaccgacgt gaggtcgccg agtgcgttga tgtcgaagac ggcggcggca aaggccacga
    10681 gcacaccgac gatcagcgtg ttgatccacg gcgtcttgta cttcggatgc actttggcga
    10741 gcactttcgg catcagtccg tcgcgcgaca tcgagtagaa aacgcgcgtc tggccaaaca
    10801 tcagcacgag aaccacggag gagagaccag cgatcgctcc gatcttgatg cacttggcga
    10861 gccaacccca ttccggcccg aaggagtcga cggcggtagc gactggcgcg gccacgttga
    10921 gcttggtgaa cgagatgact cccgtgagca ccgcggcggt cgccatgtag aggatcgtgc
    10981 agatgaccag cgagcccagg atgccaatgg gcatgtcctt gcgcggattg cgcgcttcct
    11041 gccctgcagt cgacaccgcc tcgaagccga tgtaagcgaa gaacacgatc atcgcggcgc
    11101 gaatgatgcc gtcgatgcca aattggtctt cgacgccggt gggctcggga atgaacggat
    11161 gccagttgtc ggtattcacg aagaacgcgc caaccgcgat gaacgccagg atcaccgtga
    11221 ccttgatcgc gactatgatg ttgttgacgg ttgcagattc ggacacgccg tacaccagca
    11281 gcgccgtgac cgcgagcacg atgaagaccg ccggcatgtt gaacatcgaa gtgacaaccg
    11341 cggcagcggg aatcgagacc accgtcgccg cgggcaggtg cacgacggat gacgcgggca
    11401 cgttgagcaa tgtgccggcg gcgagatcac gcaggtttcc ggttgccgga tcgagcacct
    11461 tcgttgccgc cgcgagcgtc gcgttgatat cggtgctgat cgggatgtcg agcgcgttga
    11521 tcagcgtgaa accgttctcc gccgctttca ccacgtcgta ctgcgcaatc gcggcggtgt
    11581 agctgccgcc gattgcggcc ttggcctcag tgacgaattg cacggccgaa ccgtccgcga
    11641 gcgccgcggt gacgccgttc aagctcactg aagggtcgac cacagtgatg gtcgacagtg
    11701 ccgctacgac gagttcgcca tagggctgcg tgagcgccgc cgggagattc cagcctatgt
    11761 cgtgcaggag gctcaccgcg tagcccgacc agccaaccgc gaccaccgac gcggccaatg
    11821 catattcgag caacaggagc gcgcccatga tccaggcgcc gaattcgccg acggtcgtgt
    11881 atgcgtaggt atacgcagaa ccggatacgg gaagcatcga ggcaagctcg gcgtagcaca
    11941 gcccggcgaa tgcacagacg acgcccgcga gcacgaagga caacatgacg gcgggacccg
    12001 cgtactgcgc agcggcggtg ccggtgcgca cgaatattcc cgcgccgatg atgcaaccga
    12061 tgccaaagag tacgaggtgg atggggccca gcgtgcgctt gagctcgcca cgatcaaaat
    12121 ctttctggac ctgatcaatc gacttggtac gccaacccat cgggcacccc gcatttttgt
    12181 aatggccgga ttgtagggag gccgctcgcg ttaagcgagc aggattggac gcgctccggc
    12241 gagccctagc cgccctgggt ctcgtctttc ttgttcctgc gctcatccat gtggcgtcga
    12301 aggcggatat tcaccatttc gacgcccacc gagaacgcca tggcgaagta caggtatccc
    12361 ttcggcacgt cgaagtggaa ggagtcggcc accagcacca cgcccacgag gatcaggaag
    12421 gccagcgcca ggatcttgat ggtcgggtgc cgctcgatga aggcgccgat gctgcccgac
    12481 acccacatca tgacgccgat ggccagcacg atggctgcga ccatgatggg cagctgatcc
    12541 ggcttggcga gaccaaccgc cgtgaacacc gaatcgagcg agaacacgat gtcgatgatg
    12601 gcgatctgca ccacgatgac ggtgaagttc gcgaacacgc gcgccttgcg atcgtcttca
    12661 gcgccttcga gggtcccgtg gatttcggtg acgctcttgg cgagcaggaa caggccgcct
    12721 aacaagagaa tgatgtcgcg gccggagaat tcctgcccga ggatcgagaa ccacggcttg
    12781 atgagccgcg tcagccacac gatggagaac agcagcgcga tgcgcgtgag catcgcgagc
    12841 gcgagcccgg tgatgcgcgc cgcttcgcgc cgctccggcg gcagccggct caccaggatg
    12901 gagatgaaga tgatgttgtc gatgccgagc acgatctcca gcgccgacag cgttatgaac
    12961 gctatccacg catgcggatc ggccagcagt tccatcacgg cgcggaattc gcccggttac
    13021 ggcaactcat ctatcggatc cttgcggacc gtcgggaaca ggtgctcggc cttgaggccg
    13081 cacgacagcg cgatggcgct cgaaatgtag atggacgaat acgtgccggc aacgatgccg
    13141 atgagcagcg cctcggagaa gccgcgcagc gccgagccgc ccaacaacag caacgccacc
    13201 acgacgatgg aggtgaccag cttggtcatg atggtgcgcg acagcgtctg gttgatggcc
    13261 tggtcgagcg tgacgtcgga agcctgccgg cggttgtgct cgaagcgctc gcggatccgg
    13321 tcgaacacga tgaccgtgtc gttgagcgag tacccgatga cagcaagaat agccgcgacc
    13381 accgacaggt cgaacagcgt ctgcgtgacc gagaacacgc cgagcaccag gatgggatcg
    13441 tgcatcacgg c
//
LOCUS       DASQJS010000304         5155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2696073, whole genome shotgun sequence.
ACCESSION   DASQJS010000304 DASQJS010000000
VERSION     DASQJS010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2696073"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VEW08_12235"
     CDS             complement(<1..740)
                     /locus_tag="VEW08_12235"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ41553.1"
                     /translation="MTLTLAADSRGTGGLPVLFAHSFAGDLTHWDAQLEHLSQQRRAV
                     AFDFRGHGRSPGVVGRYSYDELAKDIGAIADAQELDRFVLVGHSMGAAIATKFAARHS
                     RRVEALVLVDPPPAPGAVPAAQLQQIHEALEQDPYAVVEQFWNQQMFVDSLSDVQQRL
                     LAGLRKTPRRVAIELTREAFAVDPSIPLRRFAGPKFAIVTPRNDTPLSLHRAVPGVEH
                     VVVRGTGHWIQLDNPDAFNETLNGILKSV"
     gene            complement(815..1324)
                     /locus_tag="VEW08_12240"
     CDS             complement(815..1324)
                     /locus_tag="VEW08_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HYJ41554.1"
                     /translation="MKRMIVLSTFALATAAGAADEKYPVRLPAPGGEVVLPDAGAKKA
                     HDEWRYAAARRVGDMIYVSGVIVNRHPGEGNDVAAFKLQARRALERLRVILEAAGTDF
                     QHVAMINSFHVWQGPNFAGTRDEQFQAFESVIGEFMKAPYPAWTAVGTTGLLSDGGII
                     EVQLIAKAR"
     gene            complement(1344..3293)
                     /locus_tag="VEW08_12245"
     CDS             complement(1344..3293)
                     /locus_tag="VEW08_12245"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ41555.1"
                     /translation="MIYDAAFYAQFAPRTALDMVNQTPGFSFDGGDDETRGFSGAVGN
                     VLIDGERLGAKSQSLEDVLLRVPASEVLRIEILRGTDVAGDASNAAVLANVIRTRTAG
                     GGTWMAGFEMTNEDNPTPTGRFAWSGRSEDREYSVGATTYAHDHNSEGPREVTDGTGA
                     RVADRFGGFPHEQAEHALNGQYSQGVGDGKLVVTGQASYVRYAEDFWLRTTAPDGTQL
                     ENEVDPYRENTRSGEAGVTYQSTVADWDMALTGLVTRKRYESDVVATHFDGDNVQDSE
                     FTQALRQHSGETIARATFTRNLTVGRLEAGAEVAVNTLDGEMDLTADEGAGPVPVPIP
                     NSNLSVEENRGEAFVSHAWQINPLWSLDSRLAAETSRLSFTGDTEQSVSLTYVKPRVQ
                     LTRKFGQHQLQMRVFRDVGQLDFTDFVSTARLADDLINGGNPDLRPQTAWAAEVEGDL
                     RFASDAALRVRLFHHWLDDVVDFVPVGPPDALIDAPGNIGQGTTDGVELSLRLPLQPL
                     LPGGTLTVAGTWQETDAVDPLTAEHRQISDFVENDLKVELRQDLNAAKLAWGLTFQGY
                     SADIDFRSSEIDSFRQLHKLDAFIETTWIANTKIRLVLENVLDDFEERDRRFYTPDRN
                     GVLSLRETASFYPDMWWMFTISGNF"
     gene            3565..3927
                     /locus_tag="VEW08_12250"
     CDS             3565..3927
                     /locus_tag="VEW08_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41556.1"
                     /translation="MKNRCLVTLFAGLLCLCLGACFPEDADDPPSWIDYSGPTPTFSI
                     GGTVSGIAGVLTLQNSNGALLSVAADGSFAFETAMVSKALYNVTVAVPPQAQTCTVAN
                     GSGTVGSASITDVLITCR"
     gene            3979..4836
                     /locus_tag="VEW08_12255"
     CDS             3979..4836
                     /locus_tag="VEW08_12255"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYJ41557.1"
                     /translation="MPARVTPLDTPMKEFTVSRLLDTGTVSVRNVDCQGTCRHRSAEE
                     CASHTHFVYPYRGVYLRHVGGDQAVADANHVLFFNAAQGYRVSHPVTGGDASLVLSVD
                     ETTLRELAPKQLVCEGAGFAFNEQHLRIDARAQALVMLLKHSLQNGSIEPLEAEGLSL
                     TLVSRTLGPRTSRAPGASYARRRLVDRVKVLLAGDLSRRWTLGEIAAEMKGSPVYLTQ
                     VFQQAEGLPLYRYHLRLRLARALDLIAEREDLSGLAQDLGFSSHSHFSAAFKQHYGVT
                     PGEFRTNAR"
BASE COUNT          848 a         1600 c         1765 g          942 t
ORIGIN      
        1 acggacttca atattccgtt gagcgtctcg ttgaacgcgt cgggattgtc gagctggatc
       61 cagtgcccgg tacccctcac gacgacatgt tccacaccgg gcaccgcgcg atgcaggctg
      121 agcggcgtgt cgttccgtgg cgtgacgatg gcgaacttcg gaccggcaaa gcggcgcagt
      181 ggaatcgacg ggtcgacggc aaaagcctcg cgggtcagct cgatggccac cctgcgaggc
      241 gtcttgcgta aacctgcgag caggcgctgc tgcacgtcgg aaagcgaatc gacgaacatc
      301 tgttgattcc agaattgctc gacgacggcg tacggatcct gttcgagtgc ctcgtggatc
      361 tgttgcagct gcgcggcggg aaccgcgccc ggtgccggag gaggatcaac aaggaccagc
      421 gcctcgaccc gccgtgaatg cctggcggcg aatttggtgg caatcgccgc gcccatgctg
      481 tgacccacca gcacgaagcg gtcgagttcc tgggcgtcgg cgatcgcgcc gatgtccttc
      541 gccagttcgt cgtaggaata gcgcccgacg acgcccggcg agcggccgtg cccgcggaaa
      601 tcgaaggcga ccgcgcggcg ctgctggctc aggtgctcga gttgggcgtc ccagtgggtg
      661 agatcgccgg cgaaggagtg cgcaaacagc acgggcaaac cgcccgtgcc gcggctgtcg
      721 gcggccaatg tcagagtcat ggccgggcaa gttgcccgag tcgcggggta gaaatctgtc
      781 ggcgttcgcc gctagtgcgg gccgcgctgc gaagtcagcg cgccttggcg atgagctgca
      841 cttcgatgat gccgccatcg gagagaagcc cggtggtgcc gaccgcggtc catgccgggt
      901 acggagcctt catgaattca ccgatgacag actcgaacgc ctggaactgc tcatcacgcg
      961 tgccggcaaa gttaggtccc tgccagacgt ggaagctgtt gatcatggcg acgtgctgaa
     1021 agtcagtgcc ggcggcttcg aggatgacgc gcaggcgttc cagcgcgcgg cgcgcctgca
     1081 acttgaacgc ggccacgtcg ttgccctcgc cgggatggcg attcacgatg acgccggaga
     1141 cgtagatcat gtcgccgaca cgccgcgcgg ccgcatagcg ccactcgtcg tgcgccttct
     1201 tcgcgcctgc atcgggcagc accacctcac cgccgggagc ggggagccgg acggggtatt
     1261 tttcgtcggc ggcgccagcc gcggtggcca gcgcgaaggt ggatagaaca atcattcgtt
     1321 tcatcaggcg cgtccccttt agttcagaaa ttgcccgaga tggtgaacat ccaccacatg
     1381 tcgggataga acgatgcagt ttcgcgcagg ctgagcacgc cgttgcggtc gggagtatag
     1441 aaccggcgat cgcgctcctc gaagtcatcg agcacgttct ccagcaccag gcggatcttg
     1501 gtgttcgcta tccacgtggt ttcgatgaac gcgtcgagct tgtgcagctg gcggaagctg
     1561 tcgatttcgg acgagcggaa atcgatatcg gcggaatagc cctggaacgt caggccccag
     1621 gccagcttcg cggcgttcag gtcctggcgc agctccacct tgagatcatt ttccacgaag
     1681 tcgctgatct gacggtgttc ggccgtgagg ggatccacgg catcggtctc ctgccaggtg
     1741 ccggcgacgg tgagcgtgcc gccgggcagc aacggttgca gcggcaggcg tagcgagagc
     1801 tcgacgccat cagtggtgcc ctggccgatg ttgccgggcg cgtcgatgag cgcatcgggc
     1861 ggacccacgg gaacgaagtc caccacgtca tcgagccagt gatggaagag tcgcacgcgc
     1921 agcgctgcgt cgctggcgaa gcgcaggtcg ccttccactt cggcggccca tgcggtctgc
     1981 ggccgcaaat ccggattgcc gccattgatg aggtcgtcgg cgagccgcgc ggtggacacg
     2041 aagtcggtga aatcgagctg gcccacatcg cggaacacgc gcatctgcaa ctggtgctgg
     2101 ccgaatttgc gcgtgagctg cacgcgcggc ttcacgtagg ttagtgaaac ggattgttcg
     2161 gtatcgcccg taaaactcag gcgcgaggtt tcggccgcga gccgcgaatc gagcgaccag
     2221 agcgggttga tctgccatgc gtggctgacg aaggcttcgc cgcggttttc ctcgacgctc
     2281 aggttggagt tgggtatcgg gacgggcacg ggtcccgcac cttcatcggc cgtgagatcc
     2341 atctcgccgt ccagcgtgtt caccgccact tccgcgccgg cctcgaggcg gccgacggtc
     2401 agattgcgcg tgaatgtggc gcgcgcgatg gtctcgccac tgtgctggcg tagcgcctgc
     2461 gtgaattccg aatcctgcac gttgtcgccg tcgaagtgcg tggcgacgac gtcgctttca
     2521 taccgcttgc gggtcaccag gccggtcagc gccatgtccc agtccgcgac ggtgctttga
     2581 taggtgacgc cggcttcgcc gctgcgcgtg ttctccctgt acgggtcgac ctcgttttcg
     2641 agctgcgttc cgtcgggcgc ggtggtgcgc agccagaagt cttcggcgta gcgcacgtag
     2701 gacgcctgtc ccgtgacgac cagcttgccg tcgccgacgc cttgcgagta ttggccgttg
     2761 agcgcgtgtt cggcttgctc gtgcgggaag ccgccgaagc gatccgccac ccgcgcgccc
     2821 gtgccgtcgg tgacttcacg cggcccttcc gagttgtgat cgtgggcgta ggtagtcgcg
     2881 cccaccgaat attcgcggtc ttcgctacgg ccgctccagg caaaccttcc ggtgggagtc
     2941 gggttgtcct cgttggtcat ctcgaagccg gccatccacg tgccgccgcc ggcggtgcgg
     3001 gttcgaatga cattggccag gaccgcggcg ttggaggcgt cgccggcgac gtcggtgccg
     3061 cgcaggattt cgatgcgcag gacttcgctc gcgggtacgc gcagcagcac gtcctcgagc
     3121 gactggctct tggcccccag gcgctcacca tcaatgagca cgttgcccac ggcgccggag
     3181 aagccgcgcg tttcgtcgtc gcccccgtcg aacgagaagc ccggagtctg gttgaccatg
     3241 tcgagcgcag tgcgtggggc gaactgggcg tagaaggccg cgtcatagat cacgcgcccg
     3301 gactcggaag acggctgagg agcctgctgt gcatcagcgc cgtcaatgaa cataaagccc
     3361 agcgcaaggg tgcgcagcag cgaagaagcg ttgatgaccc gatctcccga ttgtttgttg
     3421 tcccagcgtg aggtccgcgg gagcgcagga agttctcgcg ggtgcggcag gcggaggccg
     3481 cgccggggca gggcgcgtat gttaccggcg aaatttgacg gcggcatgat ccgtggcacc
     3541 ggcaaattac gcgtttactt ccatatgaag aaccgatgcc tggtcaccct gttcgccggc
     3601 ttgctttgcc tgtgcctcgg cgcctgcttc cccgaggacg ccgatgaccc gccgtcatgg
     3661 atcgactatt cgggtcccac gccgacgttc tcgattggcg gcacggtgtc gggtatcgcc
     3721 ggcgtgctca cgctgcaaaa ttccaatggc gcactgctga gtgtcgccgc ggacggctcg
     3781 ttcgcgttcg agacggccat ggtgtcgaag gccttgtaca acgtgacggt cgcggtgccg
     3841 ccgcaggcgc aaacctgcac tgtggcgaac ggctcgggaa ccgtaggcag cgccagcatt
     3901 accgacgtcc tcatcacctg cagataaatg gggacagtcc ccatttgcgg gaaatggggc
     3961 cagggccata cttctggcat gcccgcccgc gtcactcctc tggatacacc gatgaaggaa
     4021 ttcacggtga gccgcctgct cgatacgggc acggtgtcgg tgcggaatgt ggattgccag
     4081 ggcacctgtc ggcatcgcag cgcggaggag tgcgcctcgc acacgcactt cgtatacccg
     4141 taccgcggcg tgtatctgcg ccacgtgggc ggtgaccagg cggtcgccga cgccaatcac
     4201 gtgttgttct tcaatgcggc gcagggctac cgggtgagcc acccggtgac cggcggcgat
     4261 gccagcctgg tgctgagcgt ggacgaaacc acgctgcgcg agctcgcgcc gaagcagctg
     4321 gtgtgcgagg gcgccggctt cgctttcaat gagcagcacc tgcgcatcga tgcgcgcgcc
     4381 caggcgctgg tgatgctgct caagcactcg ctgcagaacg gcagcatcga accgctcgaa
     4441 gccgaaggtc tttcgctcac gctggtgtcg cgcacgctcg gcccccgcac gtcgcgtgcg
     4501 ccgggcgcga gttacgcgcg ccgccggctg gtagatagag tcaaggtgct gctggcgggg
     4561 gatctttcgc gacgctggac gctgggagag attgccgcgg agatgaaggg ctcgccggtg
     4621 tatctcacac aggtgttcca gcaggcggaa ggattgccgc tgtaccgtta tcacctgcga
     4681 ttacggctgg cgcgcgcact ggatctgatc gccgagcggg aagacctgtc gggtctggcc
     4741 caggatctcg gcttctccag ccacagccat ttctccgcgg cgttcaagca gcactatggg
     4801 gtgacgccgg gggaatttcg gacgaatgct cggtgaggtg gggccccagc gagcagtcgg
     4861 cgcgcagagg ggcagtttgg gggtgtgcgg gccccagcga gcagtccgcg cacaggattt
     4921 cgttcgcttt tcgccgggcc cttcgcttgg gttggcgtcg ccagtttgct ggccgccgcc
     4981 atacgcaatg acaccttcgc gcggttgtga ggaccggaag ctagagtcct gatgaagcgg
     5041 agaggcggct tcgtgcctaa cgaggccagg tatcccggcc cgctctctca gctcctgtgc
     5101 gcggactgct cgctggggct catacgtcac cgaaccgcgc cccgctttcc cgcgg
//
LOCUS       DASQJS010000305         8307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2696075, whole genome shotgun sequence.
ACCESSION   DASQJS010000305 DASQJS010000000
VERSION     DASQJS010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2696075"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(386..682)
                     /locus_tag="VEW08_12260"
     CDS             complement(386..682)
                     /locus_tag="VEW08_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41558.1"
                     /translation="MKPENRIERFTTHWQSAFKDEPYYSEEYSWEDFDPAYRYAYKSY
                     EENPHARFEDLETRLEAGWDIAKGKSKLAWADAKQAVRSAWNSVERVLPGDSDR"
     gene            complement(861..1409)
                     /locus_tag="VEW08_12265"
     CDS             complement(861..1409)
                     /locus_tag="VEW08_12265"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HYJ41559.1"
                     /translation="MQKSKGFISAVVMVVTLAMVACASTRTQKSAGEQVDDSVTTASV
                     KSALIADPVTKAHQIDVEVFKGTVQLNGFVDTAASKSRATEVAKSTKGVTAVRNNLTV
                     KTTERGGEAVVDDGVITTKVKSALAGDPRTKAHQINVETREGAVQLSGFVDTSEAKST
                     AEELARAVDNVKSVDNELDVKK"
     gene            complement(1419..1580)
                     /locus_tag="VEW08_12270"
     CDS             complement(1419..1580)
                     /locus_tag="VEW08_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HYJ41560.1"
                     /translation="MLHYAVIFFVIALIAAVFGFAGIAAGAAGIAKILFFVFLILAIV
                     SFIANTARR"
     gene            complement(1741..2226)
                     /locus_tag="VEW08_12275"
     CDS             complement(1741..2226)
                     /locus_tag="VEW08_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYJ41561.1"
                     /translation="MHDEQLRPVLDRLLATRRAKVEDTQSGPRKGLRVLFTCHSGKNK
                     LLAAQLFANELSVDLFQIDLDRLVSKYVGDTEKNLDALFKAAEASDAVLLLDDTDHLF
                     GGATSDTEDSGRFPNLNVAQLRQRLESYDGVVLLGADDRKHIDPKLARQCDEVVELAD
                     D"
     gene            complement(2226..3053)
                     /locus_tag="VEW08_12280"
     CDS             complement(2226..3053)
                     /locus_tag="VEW08_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41562.1"
                     /translation="MPKPANRVSDVRPKQAVFAASIKQHPKRLVMLTEGDSWFSYPLN
                     ANIADYIEMMSDFSLLRLEHNGDEARDILGAGSEQLKKIKYYLKNYPVDAVLVSAGGN
                     DLVSGELGKVLNKKVNGATWQSTIKLDTLTTVLDDIVAAYARLLDARDALRPKCLVVA
                     HSYCYFQPTGRKATGPFGLGKAGPWMRPVLVAKGIDPDTEGRELARYLVDELHARLKA
                     LSAARSRFSVVDMRAALPVDNVHWADEIHPSGTGFRRLAEDHWRPLLKAQFPGRGFE"
     gene            3225..5120
                     /locus_tag="VEW08_12285"
     CDS             3225..5120
                     /locus_tag="VEW08_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYJ41563.1"
                     /translation="MRSLLIALLALGSSAAMAAPSLTTPPSAAAGSTISFKATGTGNP
                     REFVTVVPKGAREGAYFAYVYVTGTGDLKLAMPATPGDFELRLCAADSPYKTLAMKPI
                     QLIGATASVSAPASVAAGAPFEVKWTGPNNERDYIAIGETTPGGRLYLDYKYSRDGSP
                     MKLSAPEAPGNYEVRYILGVGDTVIARQAISVGSVAASVTAPPQVAAGAKFKVSWTGP
                     NNERDFVTMVKAGTKEKTYERYEYTSKGSTLDFTAPDVAGEYEIRYATGRTYSTLARA
                     KITVSAITGTVTGPAQVVAGESFKATWKGPDNARNFVTVVSKGAREGAYSGSYFYTTP
                     QNNPGSLVAPLVPGEYELRYSTAEQYVTLARAPITVTPAKSEPGKVAVSLAGGAKSGG
                     AIEIILDASGSMLQKLGTERRIDIAKRTLTKLTSSTIPAGTPFAFRVFGREVDSCQTD
                     LDVPVAPLNPAAVGQRIAALVAKNGAKTPIGASLDKAADDLKSVTGEKLIVLVTDGEE
                     TCGGDPAAAIERLRKAGIGTRVSIVGFALDDEKLATTFRRWSDAGGGAFFDAKDAAGL
                     DKSLTEALRPGFEVVNAQGQVLASGLVGGEAVTVPAGNHTVRIKGRANASKPAVVKPK
                     ETANVAF"
     gene            complement(5122..7587)
                     /locus_tag="VEW08_12290"
     CDS             complement(5122..7587)
                     /locus_tag="VEW08_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41564.1"
                     /translation="MTDSPDSKNGDPRDEGVVNVFDDFLLKERLALKLPCGKDELADP
                     CGSALAISGGGIRSASFALGVIQTFLNEKPGNAPPDSGETCFDRFDYMSTVSGGGYIG
                     GAVSWLKHHFGKGGNWRTYLGASNLGARSAEFRAAGIEGVDGSRFTWLDFFRQHGNYL
                     KPSSFSTMSLLGVALRGMLITLSVYTAILALMLWAMQVGGVLPPAGATEAWEGHPQRI
                     ESFLLFIGLIAGGAFLLFGIASWLGSIPTRATMFIGWVPPLLFVIAGVWLLFRNPDGS
                     IAINAATLHWRWPVAAGFLVAAGACLILLWWAKDDYDQGRIGSHVVHKWQYRVRIIYQ
                     QVLGPLLTGLTLLVAVWSVPHVYQFIFDKVGGALSAGLSSSLLGVAGAVYQFIAGRDK
                     KSTSSTFSTLRILLTSVLLVYGILLVAYSIVQGGEQHPVPMFGIEVPLVAVIGLAGVF
                     AGFIVNANYIGIGRMYRDRLMELFMPDKEAIRDNQWRRAGNADQFDIARLCDDEGTPQ
                     RPMHLVNCNVVMVGARQDRFRGRGGDSFVLSPRFSGSSATGWIETSKLGDGHFTLATA
                     VASSGAAASPHAGVAGRGITRNPLVSLLLSLTNVRLGYWIRNPRASQEPKYWHKWFPP
                     NLLAPGIRQGLFGSGTTENAFFIELTDGGHFDNTGLYELIRRRVKIIVLSQGSQDVNY
                     SMDDLANTIQRVRVDFGVHIRFTSDAYPLDAIRPAELGAVVRRGWALGTIKYPGTDKQ
                     GILLYLQASPIAGMNADTVSYWRRNVDFPNQTTADQFFDEEQLEAYRELGMGIAQTAV
                     KALKGEHEPWDEPMTALRRTFGW"
     gene            complement(7584..>8307)
                     /locus_tag="VEW08_12295"
     CDS             complement(7584..>8307)
                     /locus_tag="VEW08_12295"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HYJ41565.1"
                     /translation="GKFAGGAFGVGGHERIEEAKRDVAKQFADGDREIDITGFSRGAA
                     IAVHFANVLADEGVSINGVIEKPAVRFLGVWDVVAAFGIPINLGFNFNRVNLGYKLGL
                     SKGVKFCYHALALDEARQAFRPTRMKDGCEVWFRGVHSDVGGGNDNHALNDITLRWML
                     RKAIASGLPMVADCVGTACTRANVDGKIGNNFDPIKNRAREPKADDRFHYTVSLRPKH
                     VNPPDPCTRETELLEGQPWNPA"
BASE COUNT         1575 a         2685 c         2569 g         1478 t
ORIGIN      
        1 ccgcgggaaa gcggggcgcg gttcggtgac gtatgagccc cagcgagcag tccgcgcaca
       61 ggagctgaga gagcgggccg ggatacctgg cctcgttagg cacgaagccg cctctccgct
      121 tcatcaggac tctagcttcc gctcctcaca accgcacgaa agtgtcattg cgtgtggcgg
      181 cggcccgcaa gctagcgatg ccaacccaag cgaagggccc ggcgaaaagc gaacgaaatc
      241 ctgtgcgcgg actgctcgct ggggcccgca cacccccaaa ccgcccctct ttacgaaacc
      301 ccagcgatta gcgaacgaaa tcctgtgcgg gggatgctcg ctggggccca gctatccatc
      361 ccccgccctc ccatttccgc gttagttagc gatccgaatc gcccgggagc acgcgctcaa
      421 cgctgttcca ggccgagcgc acggcctgct tggcatcggc ccaggccagc ttcgatttgc
      481 ccttggcgat gtcccagccc gcctcgaggc gcgtctcgag atcctcgaat cgcgcgtggg
      541 gattctcttc atacgacttg tacgcataac gatatgcggg gtcgaagtct tcccagctgt
      601 attcctcgct gtagtaaggc tcgtccttga aggcgctctg ccaatgggtg gtgaaccgtt
      661 cgatgcgatt ctcgggtttc atggagatct cctgttgctg gaaggccgcg atgccgcggt
      721 gggatcacac tctgccgagg tggcgtgaac gacggagtcg gcgtgcggtt cggattcctg
      781 taggccaaaa taaaacgggg ccggagtgtc tccactccgg ccccgctcca atcacccggc
      841 attccgggtt tggctgagga ctacttcttg acgtcgagtt cgttgtccac ggacttgacg
      901 ttgtccaccg cgcgggccag ttcctcggcc gtcgacttcg cttccgaggt atcgacgaag
      961 ccagagagct gcactgcgcc ttcgcgcgtc tctacgttga tctgatgcgc cttggtgcgc
     1021 ggatcgccgg cgagagcgga cttcaccttg gtggtgatga cgccgtcgtc gactaccgct
     1081 tctcctccgc gctcagtcgt tttcacggtg aggttattgc gaacggcggt aacgcccttg
     1141 gtgctcttgg ccacttcggt cgcgcgcgac ttgctcgccg cggtatccac gaacccgttc
     1201 agctgaacgg tgcccttgaa cacttcgacg tcgatctggt gcgccttggt gaccggatcg
     1261 gcaatcaacg ccgatttcac gctggccgtg gtcacggaat cgtcgacctg ttcaccggcc
     1321 gacttctgcg tgcgcgtgga tgcgcaagcg accatcgcga gggtgacgac cattacgacc
     1381 gcactgataa agcctttgga tttctgcata tgaacctctt atctccgagc cgtattggca
     1441 atgaaactga cgattgcaag gatgaggaag acgaagaaca gtatcttcgc gattcccgct
     1501 gcgcccgcgg cgatgccggc aaagccgaac acggctgcga tgagcgcgat gacaaaaaag
     1561 atgactgcat agtgaagcat cgacttctcc gctgttttga caccgatact cagtgccggc
     1621 tgagcgcatc attgccgtta cgcaatttgc gcgatgcagg gtggcgggcg taaggcgcgt
     1681 aggcgcacac ccacaggaag gtaagcagcg gggattacaa gcgtaggacg agggtcgacc
     1741 tcagtcgtcg gcgagctcga cgacctcgtc gcactggcgc gcgagcttcg gatcgatgtg
     1801 tttgcggtca tccgcgccta acaacacgac cccgtcatac gattcgaggc gctggcgtag
     1861 ctgggcgaca ttgagattcg ggaagcggcc cgaatcctcc gtgtccgatg tcgctccccc
     1921 gaacaggtga tccgtgtcgt cgagcaacag cactgcgtcc gaggcctcgg ccgccttgaa
     1981 cagcgcgtcc aggtttttct ccgtgtcgcc cacgtacttg ctgacgagcc ggtcgaggtc
     2041 gatctggaac agatccaccg acagctcatt cgcaaacagt tgagccgcga gcagcttgtt
     2101 cttgccggaa tgacaggtga acaacacgcg caaacccttg cgcgggccgc tttgcgtgtc
     2161 ctcgactttc gcgcgccgcg tcgccagcag gcgatcgagc accggacgca gttgttcgtc
     2221 gtgcatcact cgaaaccgcg cccgggaaac tgcgccttga gcaacgggcg ccagtgatct
     2281 tccgccagcc ggcggaaccc ggtgccgctg ggatgaattt cgtcggccca gtggacgttg
     2341 tccaccggca gcgcagcgcg catgtcgacc acgctgaagc gcgaccgcgc cgccgataac
     2401 gctttcaaac gcgcatggag ttcatcgacc aggtacctgg ccagttcgcg gccctcggtg
     2461 tccggatcga tgcccttggc gacgagcacc gggcgcatcc acggaccggc cttgcccagg
     2521 ccgaagggcc cggtcgcctt gcgaccggtc ggctgaaagt agcaatagct gtgcgccacc
     2581 accaggcact tcggccgcag cgcatcgcgc gcatcgagca ggcgggcgta ggcggcgacg
     2641 atgtcatcga gcacggtagt tagcgtgtcg agcttgatgg tgctctgcca ggtggcgccg
     2701 ttcaccttct tgttgagcac cttgcccagc tcgcccgaca ccaggtcgtt gccgccggcg
     2761 ctcacgagca cggcgtccac gggatagttc ttgaggtaat acttgatctt cttgagctgt
     2821 tcactgccgg cgccgagaat gtcccgggcc tcatcgccat tgtgttccag ccgaagcaac
     2881 gagaagtcgc tcatcatttc gatgtaatcg gcgatgttgg cgttcagcgg ataactgaac
     2941 caggaatcgc cttccgtaag catgaccagc ctcttcggat gctgcttgat gctggcggcg
     3001 aacaccgcct gcttcggacg aacgtcggag acccgattgg ccggcttcgg catatgacta
     3061 ccctccctgt tacctgacct gacgctacca tccataccgc atctgcccgg tacgcatcaa
     3121 tacgcgccgg gcccgcatct gccaaaagca tgcggaaagt ccacgcaaca cgcgttcgag
     3181 ggaaactctt acactctgcg cttcattcaa tggagtcacc gacgatgcgc tccctcctga
     3241 tcgccttgtt agctctcggc agcagtgccg cgatggccgc gcccagcctc accactccgc
     3301 caagcgcggc cgcgggatcc accatctcgt tcaaggcaac gggcaccggc aacccgcgcg
     3361 agttcgtcac ggtggtgcca aagggcgctc gcgaaggcgc gtacttcgcc tacgtgtacg
     3421 taaccggcac gggcgatctc aagctcgcca tgccggcaac cccgggtgac ttcgaactgc
     3481 gactgtgcgc ggcggacagc ccatacaaga cgctggccat gaagccgatc cagctcatcg
     3541 gtgcaaccgc cagcgtgagc gctcctgcat cggtcgctgc cggcgcacca ttcgaagtga
     3601 agtggaccgg acctaacaac gagcgggact acatcgccat cggcgaaacc acgcccggcg
     3661 gacggctcta cctggactac aagtattcgc gcgacggcag ccccatgaag ctttccgctc
     3721 ccgaggcacc gggcaactac gaagtgcgct acatcctggg cgtgggtgat accgtcattg
     3781 cccggcaggc catctctgtc ggcagcgtcg ccgcctcggt aacggcgcct ccccaggttg
     3841 ccgctggcgc aaaattcaag gtgagctgga ccggcccgaa caatgaacgt gacttcgtca
     3901 ccatggtcaa ggcaggcacg aaagagaaaa cctacgagcg ctacgagtac accagcaagg
     3961 gcagcacgct ggacttcacc gcgccggatg ttgccggcga gtatgagatc cgttacgcca
     4021 cgggcaggac gtacagcacg ctggcccgcg cgaagatcac ggtcagcgcc atcaccggca
     4081 cagtaaccgg cccggcacag gtggtggcag gcgaatcctt caaggctacg tggaagggtc
     4141 cggacaatgc gcgcaacttc gtgaccgtcg tgtcgaaagg cgcccgcgaa ggcgcgtaca
     4201 gcggctccta cttctacacc acgcctcaga acaaccccgg cagcctcgtc gctccgctcg
     4261 ttcccggcga atacgagctg cgttactcca ccgcggaaca gtacgtcacg ctggcgcgcg
     4321 cgccgatcac ggttactccg gcgaagagcg agcccggcaa ggtcgcggtg agcctggccg
     4381 gcggcgcgaa atccgggggt gccatcgaaa tcatcctcga cgcctccggc agcatgctgc
     4441 agaagttagg caccgagcgg cgcatcgata ttgccaagcg aaccctgacc aaactcacct
     4501 cctccaccat tccagcggga acgccgttcg cgttccgcgt gttcgggcgt gaagtggatt
     4561 cgtgccagac ggatctcgat gtgccggtgg caccgctcaa tcccgccgcg gtcggccagc
     4621 gcatcgccgc gctggtcgcc aagaacggcg ccaagacgcc gatcggcgct tcgctcgaca
     4681 aggccgcgga cgacctcaag agcgtcacgg gcgaaaagct catcgtgctg gtcaccgacg
     4741 gcgaagagac ctgcggcggc gatcccgccg cggccatcga gcgcctgcgc aaggccggca
     4801 tcggcacgcg cgtcagcatc gtgggtttcg cactcgacga cgagaaactg gccaccacgt
     4861 tccggcgctg gtcggatgct ggcggtggcg cgttcttcga tgccaaggat gccgccggtc
     4921 tcgacaaatc attgaccgaa gcacttcgtc ccgggttcga ggtggtgaat gcgcaaggcc
     4981 aggtgctcgc cagcggcctg gtcggcggcg aagcggtcac ggtgccggcg ggcaatcaca
     5041 ccgtgcgcat caaggggcgc gcgaacgcgt cgaagcccgc cgtagtgaag ccgaaggaaa
     5101 ccgcgaacgt tgcgttttga actaccagcc gaaggtgcgg cgcagcgccg tcatgggttc
     5161 atcccacggt tcgtgttcgc ccttcagcgc cttgacggcc gtctgcgcga tgcccatgcc
     5221 gagctcgcga taggcctcga gttgctcttc gtcgaagaac tggtcggcgg tagtctgatt
     5281 cgggaaatcg acgttgcggc gccaatacga cacggtgtcg gcgttcatgc cggcgatggg
     5341 tgacgcctgc aggtacagca ggatgccctg cttgtccgtg cccggatact tgatggtgcc
     5401 aagcgcccag ccgcgcctga cgaccgcgcc aagctccgcg ggacggatgg cgtcgagtgg
     5461 ataggcatcg ctggtgaaac ggatgtgcac gccgaaatcg acgcgtacgc gctggatggt
     5521 gttggcgaga tcgtccatcg aatagttgac gtcctggctg ccctgcgaca gcacgatgat
     5581 cttcacgcgg cgccggatca gttcatacag gccggtgttg tcgaagtggc cgccgtcggt
     5641 caattcgatg aagaaggcgt tctccgtggt gccgctgccg aacagcccct gccgaatgcc
     5701 cggcgccagc aggtttggtg gaaaccactt gtgccagtac ttcggttcct gtgacgcacg
     5761 cggattgcgg atccagtagc cgaggcgcac gttggtcagt gacagcaaca acgacaccag
     5821 cggattgcgc gtgatgccac ggccggcgac accggcgtga gggctggcgg cagcgccgga
     5881 agacgcgacg gcagtcgcca gcgtgaagtg cccatcgccg agcttgctgg tctctatcca
     5941 cccggtggcg ctgctgccgc tgaaccgcgg gctcagcacg aaactgtcac cgccgcgacc
     6001 tcgaaaccga tcctggcgcg cgcccaccat gaccacgttg caattgacga gatgcatcgg
     6061 ccgctgcggc gtgccctcgt catcgcagag tctggcgatg tcgaactggt cggcgttgcc
     6121 ggcgcgacgc cactggttgt cgcggatggc ttccttgtcg ggcatgaaca gctccatcag
     6181 ccggtcgcga tacatgcgtc caatgccgat gtagttggcg ttcacgatga agccggcgaa
     6241 caccccggcg agaccgatga cggcgaccag cggcacttcg attccgaaca tgggcaccgg
     6301 gtgctgctcg ccgccctgaa cgatcgaata ggccacgagg aggatgccgt ataccagcag
     6361 cacggaggtc aacaggatgc gcagcgtgga aaatgtggat gaagtggatt tcttgtcgcg
     6421 accggcgatg aactggtaga ccgcgccggc cacacccagc agcgaactgg acaagcccgc
     6481 cgacagcgcc ccaccaacct tatcgaagat gaattgatag acgtgcggca cgctccatac
     6541 cgccaccagc aaagtgagcc ccgtgagcag gggccccagt acctgctgat agatgatgcg
     6601 cacgcggtac tgccacttgt gaaccacgtg cgaaccgatg cgcccctgat cgtagtcatc
     6661 tttcgcccac cacaggagga tcaggcacgc accggcggcg accaggaagc cagcagccac
     6721 gggccagcgc cagtgcagtg tcgcggcgtt gatcgcgatg ctgccatccg ggttgcgaaa
     6781 caacagccac acgcctgcaa tcacgaacag cagaggcggc acccagccaa tgaacatcgt
     6841 ggccctcgtg ggaatgctgc ccagccagct cgcgatgccg aacaacagga aggcgccgcc
     6901 ggcgatcagc ccgatgaaca gcagaaaaga ctcgatccgt tgcggatgac cttcccacgc
     6961 ttccgttgct cccgccggcg gcagtacgcc gcccacctgc atcgcccata acatcagcgc
     7021 caggattgcg gtgtacaccg acagcgtgat cagcatgccg cgcaatgcca caccgagaag
     7081 cgacatggtc gaaaagctcg acggcttcag atagttgcca tgctggcgga agaaatcgag
     7141 ccacgtgaat ctgctgccgt ccacgccttc gatgccggcg gcgcggaact cggcgctgcg
     7201 cgcgcccagg ttgctggcgc ccaggtacgt gcgccagttg ccgcccttgc cgaaatgatg
     7261 cttgagccag gacaccgcgc cgccgatata gccgccgccg gaaaccgtgg acatgtagtc
     7321 gaaccggtcg aaacaggtct cgcccgagtc cggcggtgca ttgccgggtt tctcgttgag
     7381 gaaggtctgg atcactccca gcgcgaatga cgccgaacgg atgccgccgc cggaaatggc
     7441 cagcgcggac ccgcagggat cggccagttc gtccttgccg catggcagtt tgagcgcgag
     7501 acgttctttc agcaggaaat cgtcgaagac attgactacc ccttcgtccc tgggatcgcc
     7561 attcttcgaa tctggactgt cggtcatgcg gggttccagg gctgtccttc gagtagttcg
     7621 gtctcgcgag tacacggatc cggtggattc acatgtttcg ggcgcagcga caccgtgtag
     7681 tgaaaccggt cgtcggcctt gggctcgcgc gcgcgattct tgatgggatc gaagttgttg
     7741 ccgatcttgc cgtcgacatt ggcccgcgtg caagccgtcc cgacacaatc ggcgaccatg
     7801 ggaaggccgg aggcaatggc cttgcgcagc atccagcgca gcgtgatgtc gttgagcgca
     7861 tggttgtcgt tgccgccgcc cacgtccgag tgcacaccgc ggaaccacac ctcgcagccg
     7921 tctttcatgc gcgttggccg gaacgcctgg cgcgcctcat cgagcgccag cgcgtgataa
     7981 cagaacttca cgcccttcga caggccgagc ttgtagccga gattgacgcg gttgaagttg
     8041 aacccgaggt tgatgggaat gccgaaggcg gccaccacgt cccacacgcc gagaaagcgc
     8101 accgctggct tttcgatgac accgttgatg ctcacaccct cgtcggcgag cacgttggcg
     8161 aagtggaccg cgatcgccgc gccgcgactg aaccccgtga tgtcgatttc gcgatccccg
     8221 tcggcgaact gcttcgctac gtcccgcttc gcttcctcga ttctttcgtg gccgccgaca
     8281 ccgaacgcgc cgccggcaaa cttgccg
//
LOCUS       DASQJS010000306        11116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2071343, whole genome shotgun sequence.
ACCESSION   DASQJS010000306 DASQJS010000000
VERSION     DASQJS010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11116
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2071343"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VEW08_12300"
     CDS             complement(<1..1074)
                     /locus_tag="VEW08_12300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ41566.1"
                     /translation="MLKQLSRARLIWLALIILLAAFFLYRCTAGGADAPEYQTAVAER
                     GNVVSRVSTSGSLQAVVTVDVGSQVSGRIQELFADFNSPVKKGQKIAKIDPSLFGAAV
                     VQAEANVMAARANVHRLTITAEDSERQAKRASEVYELRLISETERDNAVATARSARAA
                     VDQAQAQLAQSRAQLEQAQTNLRYTDIVSPTDGVVISRAVNVGQTVAASLSAPVIFTI
                     AQDLRKMEVHTNVAESDIGRLKPGMRVSFTVDAYPGEPFRGSIRDIRNAPQVVQNVVT
                     YDAVIDVENPELKLKPGMTATVSVVTDRRNDVLAVPNAALRFRPEGAPAQGAATGAGA
                     GAGQGARSGQGGAAGGEGNRRRER"
     gene            complement(1182..3830)
                     /locus_tag="VEW08_12305"
     CDS             complement(1182..3830)
                     /locus_tag="VEW08_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ41567.1"
                     /translation="MQPVRLVLVEDMPMEAEIAIRQLESGGFSCTWKRVDSEAGLRAV
                     LRELKPDIILSDFTLPGFDGISALEISLELAPDTPFIFLSGTLGEERAIDALQRGAYD
                     YVLKANLARLVPSVRRALNDSAIRRGRLALEQQLRDIVATSQDWIWEHDRAGKFTFCS
                     ESVRSTLGYAPEEILGTNASQYVHPEDLAALDFAMHTLGPNQRTASNLQARWRHRNGS
                     YRWLERNMLALLGDDGQVTGYRGSERDFSERRRQEKHISRLTRVLKMLSGVNSAMVRI
                     RQRREILAEACRLATSVGGYASAMVALVEPGTRTARPTAWSGSVDNQAAQQLTYSIGE
                     NAGEDASVIGRVLRTGASLVCNDLQTLEMGLAARASLMDSGFRSVVAYPLLVDRTPVG
                     ALMLTSYDVGAVGDEESRMLRELVANLSFALQYLHKEDEVRFLSYFDPLTGLAKRSLF
                     CERLVRSLEPRIGRRGTPAVAVLDIEHLSTFNDSFGRHVGDQLLQQVADRLKRHLDST
                     ELLAHFGGGTFGLIMEAAGDEDEAVHWMQGQVVEMFRAPFNVDGRGIPVDVKCGFARY
                     PDNGNDANALVQNAEAALRSAKSTGEKYLPHRLELSSAVVSRMTMEHRLRGAVERQEF
                     ELHYQTKIDVRTRAVRGLEALVRWRDPEAGLVMPGAFLPLMETSGLIVPLGDWILRQA
                     AADLRRWHGAGLTTGRVAVNISPVQLRRRAFADHLLDLVGEWHNENVGIDIEITEGVL
                     IDDVSSAVSQLRVLRRSGIRVAIDDFGTGYSSLSRLAELPVDMLKIDRSFISGLTSSG
                     AGRTVAETIIALGRAFDMTTVAEGVETPEQFEMLEHLGCDQSQGYLHSRPLAAADIEP
                     LMKNGAAIIKKSQMGNREPTPMALRG"
     gene            4234..5367
                     /locus_tag="VEW08_12310"
     CDS             4234..5367
                     /locus_tag="VEW08_12310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ41568.1"
                     /translation="MNTNIAQNAEHKQTLLIVDDEPASMRALCDTLEYEGYKTYGFTA
                     PAEALAAMRERSYDLLLADLQMPGTNGIDLMKSAQLIDPTLVAVIMTGHGALETAIAA
                     MKAGALDYIQKPIKLTTTLPVLERALAVRQLRIEKKRLEENVRERTEELKIANRELEA
                     FSYSVSHDLRAPLRAVSVFTQSLLSDHAGELSDEGQRLLQNVNAGAAHMDRLITDLLR
                     LSQLNRQALHKQPVRFGEVARGVIADMANERAGRDIEFVIADFPTWQVDPGLMQQVFV
                     NLISNAIKFSREREKARIEIGYRMDGNTLVCFVKDNGVGFNMKYMNKLFGVFQRLHTA
                     DQFEGTGVGLSIVRRIVERHGGKVWVDGEQDQGATFFFSLPDC"
     gene            5447..5905
                     /locus_tag="VEW08_12315"
     CDS             5447..5905
                     /locus_tag="VEW08_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41569.1"
                     /translation="MNDVVLPSTDERVPRATRASVNDRIQARTLHDVSRFIGADPVFI
                     DERIVQLEREWDLERTLEANAASVSLIGLGLGLFVDRRFLLLPAAAAAFLLQHALQGW
                     CPPLPLLRRLGLRTSAEIHNEIIALRILRGDFLDRVTYPEDALASARNWI"
     gene            complement(5919..6908)
                     /gene="motB"
                     /locus_tag="VEW08_12320"
     CDS             complement(5919..6908)
                     /gene="motB"
                     /locus_tag="VEW08_12320"
                     /inference="COORDINATES: protein motif:HMM:NF006548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HYJ41570.1"
                     /translation="MAAEAKARPIYVKRVKKVAGGHHGGAWKVAYADFVTAMMAFFMV
                     MWLITAVSKDQRAAIFDYFKNPSMEPGKSVKPAPGQMGPGGASSAVINMGGGLDAPKS
                     SLKQSDAIGATVMTSPAPPIDLKSQEQKLAEAEELKKLLEKKQLDALMQDLKEAIAKS
                     QALQPFKDQLMLDITPEGLRIQILDAQNRPMFDLGSARLKGYTNDILREVTGYLRTVP
                     NRLSVTGHTDTTPYAGITGYTNWDLSTDRANAARRSMESAGLETERISRVVGLASSVL
                     FDKVNPRSAVNRRISIVVMTQQAEQDAQKTDLPAVPVPAEAPAAEVAPAAAPT"
     gene            complement(6912..7790)
                     /gene="motA"
                     /locus_tag="VEW08_12325"
     CDS             complement(6912..7790)
                     /gene="motA"
                     /locus_tag="VEW08_12325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HYJ41571.1"
                     /translation="MIQVLGSVIVLACVLGGFALEGGHLLALWHPTEILIIVGAALGA
                     FLTSNPPKVSKQAFILALTLPKGARYGRDDYVTLLKLIYDILMKIRKDGLLAIENDIE
                     QPEKSALFQKYPVILADHHMVVFITDCLRLITGGSLDPHELESLLEYELETHHKEAHE
                     PCHAVQKVADALPGFGIVAAVLGIVNTMSALEGADTATIGHKVGAALVGTFLGILVAY
                     GFVGPIASAMEHRANEESKAFEVVKMALVASVRGYAPTVAVEFARKLLFSEVRPTFAD
                     LEAHLKGKPPPGNGGH"
     gene            7989..9203
                     /locus_tag="VEW08_12330"
     CDS             7989..9203
                     /locus_tag="VEW08_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41572.1"
                     /translation="MSKRALICLSILLSTLARAAEEPQWLKDARAREGNAKPAVLKSK
                     DNWFKARVPAKVIGDIEKSEGSYTVELDIGSDQPVYCEIMPEGFDMADMVRRNLEFTM
                     GQVAEAQGKVEARSLESIDAGVFGNVPYLQTQWLYRVNDGTAQRLGGFKQISMEKNDK
                     GIYCAHVDLGYTQSFAALAKAFADTFESTTDAPAPYYEEIAVATMGGKKIGVALTTLE
                     RDADGDTKAVETTAMLVPGPAGTLHSQDAVHLEWIRTDSSLINATHFIAKDGELTTSV
                     ALNVEDNAWVVDGELDGKKYNQKLKADAQPGTWVAQALGLRKLLSADNPVGAEHTMSA
                     WLADDPGKLTDARTKVIAKAGDKRFKALATAGDMNATVTIDKATGTIDAAEMEIGGLD
                     MTIERVYTKGAF"
     gene            9203..10714
                     /locus_tag="VEW08_12335"
     CDS             9203..10714
                     /locus_tag="VEW08_12335"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYJ41573.1"
                     /translation="MAPTRVLGLAAFLIAAAPVVATAQVAESETKAFAKVDEIFADYA
                     LDNHIPGLVYGVVADGKLVYVRGIGVQDLESKRPVTADTLFRIASMTKAFTALTILKL
                     RDEGKLRLDAPAETYVEEMRGWKYPTQDSPRIRVRDLLNHTAGFVTDDPWGDRQTPMA
                     EAEFSKILREGVPFTRPPATAWEYSNLGYALLGRIITNVSGHPYADTITQSLLKPLGM
                     ESSGFVVDAAPRERRALGYRWEDNGWRLEPTMAHGAFGSMGGIQTSANDYARWVAYLL
                     SAWPPRDEANAGPVSRASVRELGQGSSFPRGFPQRVGHPGEGACPLATSYGMGFIVSV
                     GCDFGVTMSHGGGYPGYGSHVLLIPRKGLGVFALTNRTYGGPSGAVWDAAAVLTKAEL
                     FKDRVLPISEDLARAYRAVGAIYKSGSVAASKDQLAMNFLMDRDAAGWSRDLATLKKA
                     VGDCDTTAAISATGAMAGDFKWRCAHGRVVGSVLLAPTTPASIQQIKLEIQPR"
     gene            10752..>11116
                     /locus_tag="VEW08_12340"
     CDS             10752..>11116
                     /locus_tag="VEW08_12340"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ41574.1"
                     /translation="MKKALVVLFSLAYGTQSFGEDLRSEEVEFVSHGVTLSGSIVFPA
                     GEIHSAVVFVHGSGKQTRNLVWAERFAKAGIAALVYDKRGAGRSGGEYEGNQSVSEKN
                     ISLLADDAAAALRSMSSHRS"
BASE COUNT         2053 a         3541 c         3489 g         2033 t
ORIGIN      
        1 ccgctcgcgc cggcgattgc cttctccacc cgcggcgccg ccctgtccag aacgcgcgcc
       61 ctggcccgcg cccgctcccg cgccggtcgc cgcaccttgc gcgggcgcgc cttcggggcg
      121 aaagcgcaat gccgcgttcg gcacggccag cacgtcgttg cgccgatcgg tgacgacgct
      181 gaccgttgcc gtcatgccgg gtttgagttt gagctcagga ttttcgacat cgatgacggc
      241 gtcatacgtg accacgttct gcacgacctg gggggcgttg cgaatgtcgc gaatggaacc
      301 gcggaacggc tcgccaggat aggcatcgac ggtgaagctc acacgcattc cgggcttgag
      361 gcggccgatg tcggattcag cgacgttggt gtgtacttcc atcttccgca gatcctgcgc
      421 gatcgtgaag atgacgggag cggacaacga agcggccacg gtctggccca cgttgactgc
      481 gcgcgaaatc accacaccat cggtgggcga cacgatgtcg gtgtaacgaa ggttggtctg
      541 cgcctgctcg agctgcgccc gcgactgggc cagctgcgcc tgggcctggt cgacggcggc
      601 acgcgcggag cgcgccgtgg ccaccgcgtt gtcgcgttcc gtttcggaga tgagccgcag
      661 ttcgtaaacc tcggaggcgc gcttggcctg gcgctccgaa tcctcggcgg tgatggtgag
      721 acggtggacg ttcgcgcggg ctgccatgac attggcttcc gcctggacca ccgcggctcc
      781 gaacaacgaa ggatcgatct tggcgatctt ctgacccttc ttcaccggcg agttgaagtc
      841 cgcgaacaac tcctggatgc gcccggacac ctggctgccg acatcgacgg tgaccaccgc
      901 ctgcaggctg cccgaagtgc tgacacggga tacaacgttg ccgcgctccg ccaccgccgt
      961 ctggtattcc ggtgcatcgg cgccaccggc cgtgcatcga taaaggaaaa acgctgcgag
     1021 gagaatgatg agcgccagcc aaatgagccg tgcgcgggac agttgtttga gcaatttcgt
     1081 atccgatctg acgtgacgcg ctcgtcgcgc acaagtgtag atcagtcagg ggctaacgcg
     1141 ttcccctaaa aagttatccc tcgatatatc aagaaatgct gtcaaccccg gagggccatc
     1201 ggcgttggct ctcggttacc catctgagac tttttgataa tggccgcgcc gttcttcatc
     1261 agcggttcaa tgtcggcggc ggccaggggc cggctatgca gatatccctg tgactggtcg
     1321 caacccaggt gctccagcat ctcgaactgc tccggtgttt cgacgccttc ggccaccgtg
     1381 gtcatgtcga aggcacggcc cagcgcaatg atcgtctccg ccacggtgcg ccccgcgccg
     1441 ctcgaggtga gaccactgat gaagcttcga tcgattttca gcatgtccac gggcagttcg
     1501 gcgaggcggc tcagggatga gtaaccggta ccgaagtcat cgatggccac tcgaatgccc
     1561 gaccgccgca gaacccgcaa ctgcgagacg gccgaactca catcgtcgat cagcacgccc
     1621 tcggtgattt cgatgtcgat gcccacgttc tcgttgtgcc actcaccgac caggtcgagc
     1681 aggtggtcgg caaacgcgcg gcgacgaagc tgcacgggtg agatgttgac ggccacgcgg
     1741 ccagtggtga ggcccgctcc gtgccagcgg cgcaaatccg cggccgcctg cctgagaatc
     1801 cagtcgccca gtggcacgat gagcccactg gtttccatca atggcagaaa cgcgccgggc
     1861 atcaccaggc ccgcttccgg atcgcgccag cgaaccagcg cttcgagacc gcgcactgcg
     1921 cgcgtgcgca cgtcgatctt ggtctggtaa tgcagctcga attcctggcg ctccaccgcg
     1981 ccgcgcaggc ggtgctccat ggtcatgcgc gacaccacgg ccgagctcag ttccagccgg
     2041 tgcggcaggt atttctcgcc ggtgctcttg gcgctgcgca acgcggcttc ggcgttctgc
     2101 acgagcgcgt tggcgtcgtt gccattgtcc gggtagcgcg cgaacccgca tttcacgtcg
     2161 acgggaattc cgcgtccatc cacgttgaat ggcgcgcgga acatctcgac cacctgcccc
     2221 tgcatccagt gcacggcttc gtcttcgtcg ccggcggctt ccatgatgag accgaacgta
     2281 ccgccgccga agtgcgccag cagctcggtc gagtccaggt ggcgcttgag tcgatccgca
     2341 acctgctgca gcagctgatc gccgacgtgc cggccgaatg agtcgttgaa agtagatagg
     2401 tgctcgatgt ccagcacggc caccgccggt gtgcctcgac ggccgatgcg cggctcgagc
     2461 gaccgcacca ggcgttcgca gaacaggctg cgcttggcga gacccgtgag cgggtcgaag
     2521 tacgacagga agcgcacttc gtcttccttg tgaaggtatt gcaacgcgaa tgacaggttg
     2581 gcgaccagtt cgcgcaacat gcgcgactcc tcgtcgccga ccgcaccaac gtcgtatgaa
     2641 gtgagcatca gcgcgccgac gggcgtgcgg tccaccagca gcggataggc aacgacgctg
     2701 cggaagccgg agtccatgag cgaggcacgc gctgccaggc ccatctccag cgtctgaaga
     2761 tcgttgcaga ccagcgaagc gccggtgcgc aacacgcggc cgatgacgct cgcatcttcg
     2821 ccggcgtttt cgccaatgct gtaggtgagc tgctgggccg cctggttgtc cacgctgccc
     2881 gaccacgccg tgggacgcgc ggtgcgtgtg ccaggctcga ccaatgccac catggcgctc
     2941 gcgtagccgc cgaccgaggt agcgagccgg cacgcttcgg ccaggatctc gcgccgctgc
     3001 cgtatgcgca ccatggcgct attcacgccg ctcagcatct tcagcacgcg cgtcaggcgg
     3061 ctgatgtgct tttcctgccg gcggcgttcg gaaaaatcgc gctcactgcc gcggtacccg
     3121 gtcacctggc cgtcatcgcc cagcagcgcc agcatgttgc gttcgagcca gcgatagctg
     3181 ccgttgcgat ggcgccagcg cgcctggagg ttgctcgccg tgcgctggtt cggcccgagc
     3241 gtatgcatgg cgaaatcgag cgccgccagg tcttcggggt gaacgtactg cgaagcgttg
     3301 gtcccgagga tttcctccgg cgcatatccg agcgtgctgc gcacggactc gctgcagaaa
     3361 gtgaatttcc ctgcgcgatc atgttcccag atccagtcct gcgaggtggc gacgatgtcg
     3421 cgcagctgtt gctcgagcgc gaggcggccc cggcggatcg ccgaatcgtt gagggcccgg
     3481 cgcacggacg gcaccaggcg ggccaggttg gccttgagca catagtcata cgcaccgcgt
     3541 tgcagggcat cgatggcgcg ttcttcgccg agcgtgcccg agagaaagat gaacggcgtg
     3601 tcgggcgcga gctcgagcga gatctccagc gctgaaattc cgtcgaagcc cggcagcgtg
     3661 aaatcggaaa ggatgatgtc gggtttgagt tcgcgcagca cggcacgaag cccggcttcg
     3721 gaatccactc gcttccaagt gcatgagaag ccaccggact cgagttgacg gatggcgatt
     3781 tccgcctcca tcggcatgtc ttccacgaga acaagtctga ccggctgcat tcgtattatt
     3841 ccccgaacta gttagtgcca gcgctccctc gcacgtcggc ccaaccgctg cttctcctgg
     3901 ctacgctgac tcttcccccc ggcatcgcag ccgtaagcag cctagtccag ttactcctat
     3961 tgggcgcgtg acgcaaccca cagacagcat cacgatgcga aagtagatag ttcatactcg
     4021 ttccagaggc gcctgaggtg ggacccgtcc gacatggcgg ctcccggaat tccgggaaca
     4081 aaaattgccc ggaattgaga gttaacccta ggtatttgtc attgaggctc aagcaggcgg
     4141 aaactaagcc gtaagtagag gtcacaaggg gtacacctac gcggaaagta cttaccacca
     4201 cccagggctt tctcagttac agatatccgg aacttgaaca ccaacatcgc ccagaacgct
     4261 gaacataagc agaccctcct catcgtcgac gatgagccgg cgtccatgcg cgcgctgtgt
     4321 gacaccctcg aatacgaggg ttacaagaca tatggcttca ccgcgccggc cgaagccctg
     4381 gccgccatgc gcgagcgcag ctatgacctg ttgctggccg atttgcagat gcccggcacc
     4441 aacggcatcg atctcatgaa gtccgcgcag ctcatcgacc cgaccctggt ggcggtcatc
     4501 atgaccgggc acggcgcgct cgaaaccgcc atcgccgcca tgaaggccgg cgcgctggat
     4561 tacatccaga agcccatcaa gctcaccacc acgctgccgg tgctcgagcg tgcactggcc
     4621 gtgcgccagc tgcgcatcga aaagaagcgc ctcgaggaaa acgttcggga gcgcaccgaa
     4681 gagctgaaaa tcgccaatcg ggagctggaa gcattttctt attccgtttc acatgacctg
     4741 cgcgccccgc tgcgcgcggt ctcggtgttc acgcagtctc tgctttccga ccacgccggc
     4801 gagctctcgg acgaaggcca acgtctcctg cagaacgtga atgccggcgc ggcgcacatg
     4861 gaccggctca tcaccgacct gctgcgcctg tcgcaactga atcgccaggc cctgcacaag
     4921 cagccggtgc ggttcggtga agtggcgcga ggggtcattg ccgacatggc caacgagcgc
     4981 gcgggccggg acatcgaatt cgtcatcgcg gacttcccca cctggcaagt ggacccgggc
     5041 ctgatgcagc aggtgttcgt gaacctcatt tccaacgcca tcaagttctc tcgcgaacgc
     5101 gaaaaggccc gcatcgaaat cggctatcgc atggatggca atacgctggt gtgcttcgtg
     5161 aaggacaacg gcgtgggctt caacatgaag tacatgaaca agttgtttgg cgtgttccag
     5221 cgcctgcata ccgcagatca gttcgaaggc acgggtgtcg ggctgtccat cgttcgccgc
     5281 atcgtcgaac gtcacggggg caaagtctgg gtcgacggcg agcaggacca gggcgcaacg
     5341 tttttcttca gcctgccgga ttgctgaatc acctgccgtt ttgctgaagc cagcctgcgg
     5401 cggcctctga cagtcccgcc cctggcgccg ggcgaccatc ccggtcatga acgacgtagt
     5461 gttgccgtcc accgacgagc gagtaccccg cgccacgcgc gcatccgtca acgatcgcat
     5521 tcaggcccgc acgctccacg atgtctcgag attcatcggc gccgatccgg tgttcatcga
     5581 cgagcggatt gttcagctgg agcgcgaatg ggacctcgaa cgcacactcg aggcgaatgc
     5641 cgcgtcggtg tcgttgattg gcctcggact gggactgttc gtcgaccgac gatttctcct
     5701 gctaccggcc gcggcagccg cctttctgct ccagcacgcg ctgcagggct ggtgcccgcc
     5761 cttgcccttg ctgcggcggc tgggtctgcg cacttccgcc gagattcaca acgagatcat
     5821 cgcgctgcga attctgcgcg gcgactttct cgaccgggtg acgtaccccg aagacgcgct
     5881 ggcaagcgcg cgcaattgga tctgaccgcg ttcgcggcct aggtcggtgc cgcagccgga
     5941 gccacttccg cagcgggagc ttccgccggc accggcacgg ccggcagatc cgtcttctga
     6001 gcatcctgct cggcctgctg cgtcatcacc acgatgctga tgcggcgatt gacggcgctg
     6061 cgcggattca ccttgtcgaa caacaccgac gacgccaggc cgacgacgcg cgagatgcgc
     6121 tcggtctcga ggccggcaga ttccatggag cgccgtgccg cgttggcgcg gtcggtagat
     6181 agatcccagt tcgtgtagcc ggtgatgccg gcgtacggcg tggtgtccgt gtggccggtg
     6241 acgctgaggc ggttcggcac cgtgcgcaga tagccggtga cttccctgag gatgtcattc
     6301 gtgtatccct tgagccgcgc gctgccgaga tcgaacatcg gccggttctg cgcatcgagg
     6361 atctggatac gcagtccctc gggcgtgatg tccagcatga gctggtcctt gaacggctgc
     6421 agcgcctggc tcttggcaat cgcttccttg agatcctgca tcagcgcatc gagctgcttc
     6481 ttctcgagca gcttcttgag ctcttcggct tcagcgagtt tctgctcctg ggacttgaga
     6541 tcgatgggcg gcgccggcga agtcatcacc gtggctccga tcgcgtcgga ttgcttgagc
     6601 gaagacttcg gggcgtccag tccgccgccc atattgatga ccgcgctgct cgctccgccc
     6661 ggccccatct gtccgggcgc cggcttcacg ctcttgccgg gctccatgct cggattcttg
     6721 aaatagtcga agatcgccgc gcgctgatcc ttcgagaccg cggtgatgag ccacatgacc
     6781 atgaaaaagg ccatcatggc ggtcacgaaa tccgcgtacg ccactttcca ggcgccgccg
     6841 tgatgtccgc cggcaacttt ctttacgcgc ttgacgtaaa tcggccgcgc tttcgcttcg
     6901 gcagccatgg ctcaatgtcc gccattaccc ggtggcggct ttcccttgag gtgtgcttcg
     6961 aggtccgcga acgtcggtcg aacttcgctg aacagcagct tgcgtgcgaa ctccaccgcg
     7021 actgtcggcg cgtaaccgcg cacgctcgcg accagcgcca tcttcactac ttcgaaagcc
     7081 ttgctctctt cgttggcgcg atgctccatc gccgaggcga tcgggcccac gaaaccgtag
     7141 gcgaccagga tgccgaggaa ggtgccgacc agcgccgcgc ccaccttgtg accgatggtc
     7201 gccgtgtccg caccttcgag cgccgacatg gtgttcacga tgccgagcac tgcagccacg
     7261 atgccgaagc ccggcagcgc gtcggccacc ttctgaaccg cgtggcaagg ttcgtgcgct
     7321 tccttgtgat gggtttccag ctcgtattcg agcaggctct cgagctcatg cgggtcgagc
     7381 gatccgcccg tgatgagtcg caggcagtcg gtgatgaaca ccaccatgtg atgatccgcg
     7441 agaatgaccg ggtatttctg gaacagcgcg ctcttctcgg gctgctcgat gtcgttctca
     7501 atggccagca acccgtcttt ccggatcttc atcaggatgt cgtagatgag cttgagcagc
     7561 gtgacgtagt cgtcgcgccc gtagcgcgcg cccttcggca gcgtgagcgc caggatgaac
     7621 gcctgcttcg acaccttcgg cgggttgctg gtcaggaatg cgccgagcgc cgcgcccacg
     7681 atgatcagga tttccgtcgg gtgccacagc gcaagcagat ggccgccttc gagcgcgaag
     7741 ccaccaagca cgcaggcaag aacgatgact gaccctaaga cctgaatcat tctgatgtat
     7801 ccgcagtgag cacgcgggcg cggtccgccc gtaccggata tcggtcgaac caagggttcc
     7861 ttaagttgag cgaccccggg gcggcaaagc cctgccgcgc tccgtgaaag cgctaaggat
     7921 ttggcgtgcg cggaaatgaa cagtacattt ctgtgaccgg gctccttgtc cggcccaacg
     7981 gaggttcgat gtcgaagcgc gcgctcattt gtttgtccat cttgctttca acgctcgccc
     8041 gggccgcgga ggaaccgcaa tggctcaagg acgcgcgtgc gcgcgagggc aatgccaagc
     8101 cagcggtgct caagagcaag gacaactggt tcaaggccag ggtgccggcg aaagtgattg
     8161 gcgacatcga gaagtcggaa ggttcgtata ccgtcgaact cgatatcggc agcgaccagc
     8221 cggtctactg cgaaatcatg cccgagggat tcgacatggc ggacatggtt cgccggaacc
     8281 tcgaattcac gatgggacag gtggcggaag cccagggcaa ggtcgaagcc cgcagtctcg
     8341 aatccatcga tgccggcgtg ttcggcaatg tgccctatct acagacacag tggctgtatc
     8401 gagtgaacga cggcacggcg caacgcctcg gtggcttcaa gcagatatcg atggaaaaga
     8461 atgacaaggg catctactgc gcccacgtgg acctcggata cacccagagc tttgccgcgc
     8521 tcgccaaggc ctttgccgac acgttcgagt ccactaccga cgcccccgcc ccgtactacg
     8581 aggaaatcgc cgttgccacc atgggcggca agaagatagg cgttgcgctc accacgctcg
     8641 aacgcgacgc ggacggggac accaaggccg tggaaaccac ggccatgctc gttcctggcc
     8701 ctgcaggtac gctgcattcg caggatgccg ttcacctgga atggatccgc accgattcct
     8761 cgctgatcaa tgccactcat ttcatcgcca aggatggaga actgacgacc agcgtcgcgc
     8821 tcaacgtcga ggacaacgcc tgggtggtcg acggcgaact ggacggcaag aagtacaacc
     8881 agaaattgaa agccgatgcg cagcccggca cctgggttgc gcaggcgctc ggcctgcgca
     8941 aactactgtc cgccgacaat cctgtcgggg ccgaacacac catgtcggcg tggctggctg
     9001 atgatcccgg gaagctgact gatgcgcgaa ccaaagtgat cgccaaggcc ggcgacaagc
     9061 ggttcaaggc gttggcaacc gccggcgaca tgaatgcgac cgtcaccatc gacaaggcga
     9121 ccggaaccat cgatgccgcg gagatggaaa tcggcgggct ggatatgacg atcgaacgcg
     9181 tgtacaccaa gggtgctttc tgatggcccc aacccgagtg ctcgggcttg ccgcctttct
     9241 cattgcggca gcaccagtgg tagcgacggc gcaggtggcg gagtccgaaa cgaaagcgtt
     9301 cgccaaggtc gacgagatct tcgcggacta cgcgctcgac aatcacattc ccggcctcgt
     9361 ctacggcgtc gtggctgatg gaaagctggt gtacgtgcgt ggaatcggcg tccaggacct
     9421 ggagtccaaa cgaccggtga cggcggatac gctgtttcgc atcgcatcga tgaccaaggc
     9481 gttcaccgcg ctcacgattc tgaagctgcg cgacgaaggc aaactgcggc tcgacgcgcc
     9541 cgcggaaacc tacgtcgaag aaatgcgcgg ctggaaatac ccgacgcagg actcaccgcg
     9601 catccgcgtg cgcgatctgt tgaaccacac cgcggggttc gtcaccgacg atccctgggg
     9661 cgatcgccag acgccgatgg ccgaagccga gttctcgaag atcctgcgcg aaggcgtgcc
     9721 cttcacccgt ccgccggcca cggcctggga atattcgaac ctgggttacg ccctgctcgg
     9781 ccggatcatc accaatgtgt ccgggcatcc atatgcggac accatcaccc agtcgttgtt
     9841 gaaacctctc ggcatggagt cttcgggctt cgtcgtcgat gccgcgccgc gcgagaggcg
     9901 cgcgctcggg tatcgctggg aggacaacgg ttggcgcctc gagcctacga tggcgcacgg
     9961 cgcgttcggc tcaatgggcg gtatccagac cagcgccaac gactacgcca ggtgggttgc
    10021 ctatctactg tccgcgtggc cgccgcgcga tgaagcgaat gccggccccg taagccgcgc
    10081 ctccgtgcgg gagctcggac aaggctcgag ctttccgcgc ggtttcccgc agcgggtcgg
    10141 tcatccgggc gaaggcgcct gcccgctcgc caccagttac ggaatgggtt tcatcgtgtc
    10201 cgtgggctgc gatttcggag tcacgatgag ccacggcggc ggttatcccg gctacggttc
    10261 gcatgtgctg ctcattccca ggaagggcct cggcgtgttc gcgctgacga accggaccta
    10321 cggcggccca tccggtgccg tgtgggacgc cgctgccgtg ctgaccaagg cggagctgtt
    10381 caaggaccgc gtgctgccca tcagcgaaga cctcgcgcgt gcataccggg ccgtgggcgc
    10441 gatctacaag tcgggcagtg ttgctgccag caaggaccag ctcgcgatga atttcctcat
    10501 ggatcgcgac gcggccggct ggagccgcga cctcgcgaca ttgaagaagg ccgtcggcga
    10561 ttgcgacacc accgcggcca tcagcgccac cggcgccatg gccggcgatt tcaaatggcg
    10621 ttgcgcgcac ggccgcgtag tcggcagcgt actgctcgcg ccgacgacgc cggcgagcat
    10681 tcagcagatc aagctggaga ttcagccgcg ctgattcaaa ccctgcattg cgtggttgcg
    10741 tctcaattcc catgaagaag gcactcgtcg ttttgttttc gctggcgtac ggcacgcaga
    10801 gcttcggtga agatctgcgc tctgaagaag tcgagtttgt cagtcacggc gtgacgctgt
    10861 ccggatcgat cgtgtttccg gccggcgaaa ttcattcggc ggtggtgttc gttcacggct
    10921 cgggaaagca gacgagaaac cttgtttggg ccgagcgctt tgcgaaagcc ggaatagccg
    10981 cgctggtgta cgacaagcgc ggcgccggaa ggtcaggcgg cgagtacgag ggcaaccaaa
    11041 gcgtcagcga aaagaacatt tcactgctgg cggacgacgc cgcagccgca ctgcgaagca
    11101 tgagcagtca tcgatc
//
LOCUS       DASQJS010000307        11014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3333935, whole genome shotgun sequence.
ACCESSION   DASQJS010000307 DASQJS010000000
VERSION     DASQJS010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3333935"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VEW08_12345"
     CDS             <1..925
                     /locus_tag="VEW08_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYJ41575.1"
                     /translation="IGLNVDRDHITMVVLDFEGKIRARASTEVLFALPKDVEQFFRKG
                     VGKLLTKVGIGRDRLVGVGVALPDDMPRAALPQQPANYGIWASTDVASLLHETLKVPV
                     FVENDAAAATMGEMQFGLGKKYQTFFYILITAALGGSLVIDGNHFRGATGRSGELGML
                     RGRDATGHERQIQNIVSLSALYSRLAAQGIRVSAPHELTGLDGSGRAIIDAWVEIAVD
                     TLLDTVLAINCLVNPEAVLIGGRLPSGIVDQIANRLNQRLAAFTGTVPAIAPVARAAL
                     SDDAPAVGAAILPFSHRLLPSRTALMKVAAL"
     gene            983..2302
                     /locus_tag="VEW08_12350"
     CDS             983..2302
                     /locus_tag="VEW08_12350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HYJ41576.1"
                     /translation="MRAGFATVTTLFFMWGFITVSVDPLIAALKAIFELTYAEVMLSQ
                     VAFFMAYFVVSLPAAGIIARLGNTRSILLALAIMIVGCLTMPVATSNNSFEIVLVGLF
                     IIAGGITILQVVANPVAAALGPPERSHFRLTFSQAFNSFGTWLAPMVVSSVLLVGGIF
                     AEGAKCETVTPAMRDESLGKIDLTFFLMAGALVLLAVFIWTFRRHLEGAPLAPSSEKR
                     PSVLTALKSKWALLGGLAIFLYVGAEVSIGSAMTNFLNYDDMLGVPLKTAGHMVAYYW
                     GGAMIGRFIGSFLLTRVPATRLLAIFAAIAALMCLAVSQLHGPTASYIALSIGLFNSI
                     MFPVIFTVTLERSTAPAAATSGLLCLAIVGGAVLPYVFGLIADVVGGPQPKLSECVGG
                     ANMASSVGLHAAYLLPMTAYAIISIFAVRAGKAPVVEQSTGQASAGH"
     gene            2315..3466
                     /locus_tag="VEW08_12355"
     CDS             2315..3466
                     /locus_tag="VEW08_12355"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HYJ41577.1"
                     /translation="MDDKARIEQLHSQLCSWLCDAAYPLWSTRGRDPAGGFHERLAQN
                     GEPLAEPRRSRVNPRQAYCFAVAPSLGWRGDSAALVKHGLDYFVARYQRPDGLFRTLV
                     AADGSSLDDRALLYDQAFALLAFNVAAAAGESRGEREHQSRELLTLVLKTLKRAGPGF
                     NSGVPPSLPLQSNPHMHLFEAALSGCEVCSESSQWKSLADELAELALARFIDSSTGAL
                     REFFDENWNPAPGTPGRIVEPGHQFEWAWLLLRWSGPKQGQVHARARAAALKLIDVGE
                     SHGLRNGLATNSLLDDFSAHDAGARLWPQTERLKAACIAARLTGEARYFSMAVAAADG
                     LLRYLDCPVPGLWYDRIDADGKLVDEPAPASSFYHLVAAIAEISALARL"
     gene            3596..4171
                     /locus_tag="VEW08_12360"
     CDS             3596..4171
                     /locus_tag="VEW08_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HYJ41578.1"
                     /translation="MKKLAQAVLLAVLSVSAVTAAQAADTGKRGGFLAQMDFDFGGDD
                     VATLSFEDGDTQDVKAGQGIGIGIGGWFRPAASVPFELHGLIGYKVVFTAAENADIKM
                     TRSTLQFSGVYRFPSNWYVGAGYVQHMSPELDGDGFFEDFEFDNASGFNAEVGWKWVS
                     LHYTRIEYSSPGYEDVDGSNVGIRLTYRFGL"
     gene            complement(4185..5201)
                     /locus_tag="VEW08_12365"
     CDS             complement(4185..5201)
                     /locus_tag="VEW08_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYJ41579.1"
                     /translation="MSRITIHDVSKAAGVSIKTVSRVLNSEPNVRSETRARVLKLVEE
                     LNYHPSLSARSLAGRSSNLIGLIYQNPSANYVVDLQKGALERCHTRGYRLLVHPCEGT
                     DLSSVRDLAGLVLESHLDGVVLAPPLSDSTEVLAELQKHRIRFVRIAPTVDLDAYSCV
                     FMNDVAAAREMTEYLIALGHERIGFINGPSGHPVTEQRYLGYTLALRSHGLDVDPQLV
                     DRGEFTFDSGLAAARRLLSRATRPTAVFASNDDMAAGTIMAAHEMGLTIPSRLSVAGF
                     DDSYVAQVIWPRLTTVHQPTYEMAHQAADMLFRSLGDETPPRSMEIPYRMVCRESTGP
                     RPAN"
     gene            5396..6433
                     /gene="glk"
                     /locus_tag="VEW08_12370"
     CDS             5396..6433
                     /gene="glk"
                     /locus_tag="VEW08_12370"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HYJ41580.1"
                     /translation="MTVNKAPAGADSEREQFDRSAVLGLVADVGGTHIRFGIAVGGAA
                     SDGIHVHCVRRYAVRAHESIAHAAQHYRSDTPGVPEALSRAAVAVAGRVDADQVQLTN
                     SAWSFSARALSESQEFEHLQVINDFEAVGHAVGALRAGDLNPIGPQRLALPMNQGVCA
                     IVGVGTGLGVGGVVANAGAFTVLATEGGHASFAPTNERAIELLRWLRRKYGRVSAERV
                     LSGAGLTNVYMALCEIEGLHVPELSASEITTRAANGSDANCAEAIGQFCELLGSFAGD
                     LALTLGAWQGVLISGAMLQHFDRPTLERRVRAGFEYKGRFTPAMRSVPVETISHPHVE
                     LLGAARLLFNS"
     gene            6430..7905
                     /gene="zwf"
                     /locus_tag="VEW08_12375"
     CDS             6430..7905
                     /gene="zwf"
                     /locus_tag="VEW08_12375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HYJ41581.1"
                     /translation="MKHPIQKFVLFGATGDLSQRMVWPSLYHLCREKLVPPTLTFIGS
                     ARHPMANDEFRKVVEGAVRKHVPAEYLKDDAVADLLTRITYAPFEASDGSAKGTEALQ
                     KALAGNNRVLYFMATSPKLYGPTSLSLAAAGLACELCRIILEKPIGTDYASSVAINEA
                     VGSVFHEDRIFRIDHYLGKEAVQNLLALRFANSLFEPLWNSSNIDNVQITVAETVGLE
                     GRWGYYDDSGALRDMLQNHMLQLLALVAMEPPHQFEPNEVRNEKTKVIRSLRPITAAM
                     AAANTVRGQYAAGSAGGVAAPGYNQETGARESPKKGSSDTETFVALRAEIDNWRWAGV
                     PFYLRTGKRMQSRRTDIAIQFKSVPHNIFAADEPILQANRLVIHLQPQESVTLEILSK
                     QPGLEGVRLREVPLNLSLPKDESGGRTRIAYERLLLDALKGNTTLFVRRDEVEAAWHW
                     IDGIQTSWRSTRQLVKPYPAGTWGPTAAIALTERHGHSWYE"
     gene            7936..9747
                     /gene="edd"
                     /locus_tag="VEW08_12380"
     CDS             7936..9747
                     /gene="edd"
                     /locus_tag="VEW08_12380"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008038612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HYJ41582.1"
                     /translation="MATQKLHDTIIEVTERIGGRSAAARGEYLERTRKRKLIEPARGK
                     LACANLAHVMAASGVDKPQLQQSVIPNIGIVTSYNDMLSAHRPYEPYPEVIRAAARVA
                     GATAQVAGGVPAMCDGVTQGRAGMELSLFSRDVIALATAVALSHEAFDSALLLGICDK
                     IVPGLFIGAASFGQMPIIFVPGGPMPSGMANKAKAEVRQRYAEGLATRDELLAAEMGS
                     YHDAGTCTFYGTANSNQMLMEMMGLHVPGAAFVAPNTPLRHALTVAATQRVAQTTALG
                     SSYLPFVEVIDERSFVNAIVGLMATGGSTNHVIHLVAMARAVGLTVTWDDMADISRVT
                     PLIARVYPNGEADVNQFHAAGGMSFVVRELINAGLAHESAMTIWGPGLSAYAKDPFLV
                     DNQLVWRDAPEKSHDEKILRPVANPFSADGGLKLLRGNLGRSVIKTSAVDPKYWDITA
                     PARVFESQEAMAAALKAGLNEDFIAVIRGQGPRANGMPELHKLIPAMSGLLAKGRRVA
                     IVTDGRMSGASGKVPAAIHLTPEGAADGPISRLRDGDIIRLDARAGVLEAQVDAATLQ
                     AREAVKPEVDTTHGVGRELFTRLREGASAADEGASIL"
     gene            9869..10978
                     /locus_tag="VEW08_12385"
     CDS             9869..10978
                     /locus_tag="VEW08_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HYJ41583.1"
                     /translation="MKTRAAIGWKAGQPLEVVTLDLAGPKTGEVLVEIKASGVCHTDE
                     FTRSGGDPEGLFPVVFGHEGAGVVVDVGPGVTTLKKGDHVIPLYTPECRQCKSCTSHK
                     TNLCTAIRATQGQGVMPDGTSRFSIGNEKVHHYMGCSTFSNYTVLPEIALAKIREDAP
                     FEKVCYIGCGVTTGIGAVINTAKVEPGANVVVFGLGGIGLNVIQGARMAGANKIIGVD
                     INPKRKAMAEKFGMTHFVNPKEVQGDLVPYLVSLTDGGADYSFECVGHVDLMRQALEC
                     THRGWGVSVIIGVAGSGQEIKTRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMDKK
                     INIDDLITHVMPIEKINDAFDLMHKGESIRSVVTF"
BASE COUNT         1987 a         3481 c         3532 g         2014 t
ORIGIN      
        1 gatcgggctc aatgtcgacc gcgatcacat caccatggtg gtgctcgatt tcgagggaaa
       61 gattcgcgcg cgcgcctcca ccgaggtgct cttcgcgctg ccgaaggacg tcgagcagtt
      121 cttccgcaag ggtgtcggca aactactcac gaaagtgggg atcggtcgcg accggctggt
      181 cggtgtcggc gtggcgctgc ccgacgacat gccgcgtgcc gcgctccccc agcagccggc
      241 caactacggc atctgggcct cgaccgacgt ggcctcactg ttgcacgaaa cgctgaaagt
      301 tccggtgttc gtggaaaacg acgccgccgc cgccaccatg ggcgagatgc agttcgggct
      361 cggcaagaag tatcagacct ttttctacat tctcatcacg gccgccctcg gtggcagcct
      421 ggtgatcgac ggcaatcact tccgcggcgc caccggccgc agtggtgagc tcggcatgct
      481 gcgcgggcgc gacgccaccg ggcacgagcg tcagatccag aacatcgtgt cgttgtcggc
      541 gttgtacagc aggctcgcgg cgcagggcat ccgcgtgtcg gcgccgcacg agctcaccgg
      601 gctcgacgga agcggccggg ccatcatcga cgcctgggtg gaaatcgccg tcgacacgct
      661 gctcgacacc gtgctcgcca taaactgcct ggtgaatccc gaagcagtgc tgatcggtgg
      721 ccgcctgccg tcaggcatcg tcgaccagat tgccaaccgg ctgaatcagc gcctcgcggc
      781 attcaccggc accgtgccgg cgatcgcgcc ggtggcgcgt gccgcgctgt cagacgacgc
      841 gccggcggtc ggcgcggcca ttctgccgtt cagccaccgg ctgctgccgt cgcgcacggc
      901 cctgatgaaa gtggcggcac tgtaaaattt cttccgttct gaaactcttc cgagggacca
      961 gatgaattct aacaaccaag gcatgcgagc tggatttgcg acggtcacca cgttgttctt
     1021 catgtggggg ttcatcacgg tctcagtcga tccgctcatt gcggcgctca aggcgatttt
     1081 cgaactgacc tacgccgaag tgatgctgtc gcaggtcgcc ttcttcatgg cgtacttcgt
     1141 ggtgtcgctg ccggctgcgg ggatcattgc ccggctcgga aacaccaggt cgatcctgtt
     1201 ggcgcttgcc atcatgatcg tcggatgcct gacgatgccg gtggcgacat cgaataacag
     1261 cttcgaaatc gtgctcgtcg gcctgttcat catcgccggc ggcatcacga ttctgcaggt
     1321 ggtggcaaat ccggtcgccg cggcgctggg gccgccagaa cgatcgcatt tccgcctgac
     1381 tttttctcag gcgttcaatt ccttcggcac gtggctcgcg cccatggtgg tgtccagcgt
     1441 gttgctcgtg ggtggcatct ttgccgaagg cgccaaatgc gagacggtga cgccggcgat
     1501 gcgcgatgag tcgctcggga aaatcgacct cacgttcttc ctcatggccg gcgcgctggt
     1561 gttactggcc gtattcatct ggactttccg gcgccatctt gaaggggccc ccctggctcc
     1621 gtcatccgag aaaaggccgt cggtgctcac ggcgctcaag tcgaaatggg cattgctcgg
     1681 cggtctcgcg attttcctgt acgtcggtgc ggaagtgtcg atcggaagcg cgatgacaaa
     1741 ttttctcaac tacgacgaca tgctcggcgt gccactcaag acggccggcc acatggtcgc
     1801 gtattactgg ggtggtgcga tgatcggccg cttcatcggc agttttctgc tcacacgcgt
     1861 gccggcaacg cggctcctcg cgattttcgc ggcgatcgcc gcgctgatgt gcctggcggt
     1921 gagccagctg catggcccga cggcttccta catcgcgctg tcgatcggcc tgttcaactc
     1981 catcatgttc cccgtgatct tcacggtgac actggagcgc agcaccgcgc ctgcggcggc
     2041 cacttcgggg ctgctgtgcc tggccatcgt cggtggcgcc gtgctgcctt acgtgttcgg
     2101 cctcatcgcc gatgtcgttg gtggtccgca gcccaagctt tccgagtgcg tcggaggcgc
     2161 caacatggca tccagcgtcg gtctgcatgc ggcctacctg ctgccgatga ctgcgtacgc
     2221 gatcattagt atctttgccg ttcgcgccgg caaggcgccg gtcgtcgaac agtcgacggg
     2281 tcaggcgtcc gcgggccact aaattcgagg tgacatggac gacaaggcgc gcatcgaaca
     2341 actgcacagc cagctttgca gctggctgtg cgatgccgcc tatccgctct ggtcgacgcg
     2401 cggccgcgat ccggccggcg gcttccacga gcggctcgcg cagaatggcg aacccctggc
     2461 cgaaccacgc cgctcccgcg tcaacccacg ccaggcttat tgctttgccg tagcgccgtc
     2521 acttggctgg cgcggtgatt cggcggcgct ggtcaaacat gggctcgact acttcgtggc
     2581 gcgttaccag cgtcccgatg gcttgttccg cacgctggtg gcggccgacg ggtcatcgct
     2641 cgatgatcgt gcgctgctgt acgaccaggc cttcgcgctg ctcgctttca acgtggccgc
     2701 tgcggccggt gaatcgcggg gcgagcgcga gcaccagtcg cgcgagttgc tgacgctggt
     2761 cctcaagacc ctgaagcggg cagggccggg cttcaacagt ggcgttccgc cgagcctgcc
     2821 gctgcaatcg aacccgcaca tgcacctgtt cgaagccgcg ctctcgggtt gtgaggtgtg
     2881 ctcggaaagc tcgcagtgga agtcgctggc cgatgagctg gccgaacttg cgctcgccag
     2941 gttcatcgat tccagcaccg gcgcactgcg tgagttcttt gacgaaaact ggaatccggc
     3001 tcctggcaca cccgggcgca tcgtcgagcc tgggcatcag ttcgaatggg cgtggttgct
     3061 gttgcgctgg agcgggccaa aacaaggaca ggtgcacgcg cgtgcacgcg cggccgcact
     3121 caagctcatc gatgttggcg agagccacgg gctgcgcaac ggactcgcga ccaacagcct
     3181 gctcgacgat ttttccgctc atgacgccgg cgcgcgcctg tggccgcaga ccgagagact
     3241 caaggcggcg tgcatcgccg ctcggctgac cggggaggcc cgctattttt cgatggcagt
     3301 ggccgccgcc gatggcttgc tgcgctatct cgactgtcct gttcccggcc tctggtatga
     3361 ccgcatcgac gccgatggca agctcgtcga cgagcccgcc cccgcgagca gcttctacca
     3421 cctggttgcc gcgatcgcgg aaatcagcgc gctcgcgcgc ctctaactaa cacccccctt
     3481 actaccggcg tgtgacgcac ttcaagaagc gcgtcacggg cgtttgacga gtgcagggaa
     3541 accacgagaa tgcggaagcc tcccgaaggg agcgcattca accgggagga actggatgaa
     3601 gaagttagct caggccgtgt tgctggcggt cctgtcggtt tccgctgtga ctgcagcgca
     3661 ggccgccgat accggcaagc gcggtgggtt cctggcgcag atggacttcg attttggcgg
     3721 cgacgacgtc gcgacgctgt ccttcgagga cggcgacacg caagacgtga aagccgggca
     3781 gggcatcggc atcggtattg gcggctggtt ccgtcccgcg gcatccgttc cattcgaact
     3841 gcacggattg attggctaca aggtggtgtt caccgccgcg gaaaacgccg acatcaagat
     3901 gacgcgctcg acgctgcagt tcagcggcgt gtatcgcttc cccagcaatt ggtatgtcgg
     3961 agccgggtac gtgcagcaca tgagccccga gctcgacggt gacggcttct tcgaagattt
     4021 cgaattcgac aatgccagcg gcttcaacgc cgaggtgggc tggaagtggg tctccctgca
     4081 ctacaccagg atcgaatact cgagccctgg ttatgaagac gtcgacggga gcaacgtcgg
     4141 cattcgcctc acgtatcgct tcgggctctg agcagcgcag cggcctagtt cgccggacgg
     4201 ggtccggtgg attcgcggca caccatgcgg tagggaattt ccatgctcct gggcggcgtt
     4261 tcgtcgccca gcgagcggaa gagcatgtct gccgcctggt gcgccatctc atacgttggc
     4321 tgatggacgg tggtgaggcg cggccagatg acctgcgcga catagctgtc gtcgaagccg
     4381 gcaaccgaaa gacggctggg gatggtgaga cccatttcgt gtgcggccat gatggtgccg
     4441 gcggccatgt cgtcattgct ggcgaacacg gcggtgggtc gtgtcgcacg agacaacaaa
     4501 cgccgcgccg cagccagccc cgaatcgaat gtgaactcgc cgcgatccac gagttgcgga
     4561 tcgacatcga gaccgtgcga acgcaacgcc agcgtgtacc cgaggtaacg ttgttcagtc
     4621 accggatgtc ctgacgggcc attgatgaag ccaatgcgtt catgccccag tgcaatgagg
     4681 tattcggtca tctcgcgcgc cgcagccacg tcgttcatga acacgcagga ataagcgtcg
     4741 agatcgacag tgggcgcgat gcgcacgaaa cggatgcggt gcttctgcag ttcggccagc
     4801 acttcggtgg agtcggacag cggtggcgcc agcactacgc cgtcgaggtg ggattcgagc
     4861 accaggcccg ctagatcgcg gacgctcgat aagtccgtac cttcgcaggg atgcactaac
     4921 aaccgatagc cgcgggtgtg gcagcgttcg agcgcgccct tctgcagatc gactacgtag
     4981 ttggcgctgg ggttctggta gatcagcccg atgagattgg agctgcggcc agcgaggctg
     5041 cgcgcagaaa ggctcgggtg atagttgagc tcttcaacga gcttgagcac gcgtgcgcgg
     5101 gtttcgctgc gcacgttcgg ttcgctgttg agcacgcgcg agacagtctt gatggacact
     5161 cccgcggcct tggaaacgtc atgaatcgtt attcggctca taccaggccc ccctgtcgac
     5221 gcagaattgt cttgttgtca gacaacgttg tcaatcaacg ccaggctttg caagacacgc
     5281 accaccgtta cgttaattac taggtagaat cgcgggccct attggttgac agcgttgtct
     5341 gaacgggcgc aggcttcgcg gtctttttcg gagtgccttt cttcagagga taatggtgac
     5401 agtcaacaaa gcgcccgccg gcgcggattc cgagcgcgaa caattcgacc gttccgcggt
     5461 gctggggctg gtagccgatg tcggaggcac tcacatccgc tttggtatcg cagtcggcgg
     5521 cgcggcatcc gacggcattc acgtccattg cgtccggcgt tatgccgttc gcgcccacga
     5581 atccatcgct catgcagcgc agcactatcg cagcgacacg cccggcgtgc ccgaagcact
     5641 gagccgcgcc gcggtcgcgg tggccgggcg cgtggatgct gatcaagtgc agctcaccaa
     5701 cagtgcgtgg tccttctcgg cgcgcgcgtt gagcgagtcg caggaattcg agcacctgca
     5761 ggtcatcaac gatttcgaag ccgtgggcca cgcggtcggt gcgctgcgcg ccggcgatct
     5821 caatcccatt ggcccgcagc gcctggcgct gcccatgaat cagggcgttt gcgccatcgt
     5881 gggcgtgggc acgggcctgg gggtgggcgg cgtcgtcgcc aatgccggcg cgttcaccgt
     5941 gctggccacc gaaggcggcc acgccagctt cgcgcccacg aacgagcgcg ccatcgagct
     6001 gctgcgctgg ttgcgccgta aatacgggcg cgtatcggcc gaacgagttc tgagcggcgc
     6061 tggtctcacc aatgtctaca tggcgctctg cgaaatcgaa ggcctgcacg ttccggagct
     6121 gtcggcgtcg gaaatcacca cgcgcgcggc caatggcagc gacgcaaatt gcgccgaagc
     6181 gatcggccag ttctgcgagc tgctgggttc gttcgccggt gacctggcgt tgacgctggg
     6241 ggcctggcag ggtgtgctga tctccggcgc catgctccag catttcgatc gcccgacgct
     6301 cgagcgtcgc gtgcgcgccg ggttcgaata caaggggcgc ttcacacccg ccatgcgcag
     6361 tgtgccggtc gagaccattt cccatccgca cgtcgagctg ctcggcgctg cgaggctgct
     6421 gttcaactca tgaaacatcc gattcagaag ttcgttctgt tcggggccac cggcgatctg
     6481 tcgcagcgca tggtgtggcc atcgctctac cacctgtgcc gtgaaaagct cgtgccgccg
     6541 acgctgactt tcatcggctc cgctcgccat ccgatggcca acgacgaatt ccgcaaggtc
     6601 gtcgaaggcg cggtgcgcaa gcacgtgccc gccgaatacc tcaaggacga tgcggtggcg
     6661 gacttgctga cgcgcatcac ctacgcaccc ttcgaagcca gtgacggcag tgcgaaggga
     6721 acggaggcgc tgcagaaggc gctcgcggga aataatcgcg ttctctattt catggccact
     6781 tcgccgaagc tctatggtcc gacgtccctg agcctcgccg ccgcgggact ggcctgcgaa
     6841 ctctgtcgca tcatcctcga gaaaccgatt ggcacggact acgcatcgag cgtcgcgatc
     6901 aacgaggccg tgggcagcgt tttccacgag gaccggatct ttcgcatcga ccactacctc
     6961 ggcaaagagg cggtgcagaa tctgctggcg ttgcggttcg cgaactcgct gttcgaaccg
     7021 ctgtggaatt catcgaacat cgacaacgtg cagatcaccg tcgctgaaac cgtgggcctc
     7081 gaagggcgct ggggctacta cgacgattcc ggcgcgctgc gcgacatgtt gcagaaccac
     7141 atgctgcaac tcctggcact ggtcgcgatg gagccgccac accagttcga gccgaacgaa
     7201 gtgcgcaacg aaaaaaccaa ggtgatccgc agcctgcggc ccatcacggc tgccatggcc
     7261 gccgcgaaca cggtacgcgg ccaatacgcc gcgggtagcg ccggtggcgt cgccgccccc
     7321 gggtacaacc aggaaacggg tgcccgggaa agtccaaaaa agggaagcag cgacaccgag
     7381 accttcgtcg cgctgcgcgc cgagatcgac aactggcgct gggcgggtgt gccgttctac
     7441 ctgcgcaccg gcaagcgaat gcagtcgcgc cgcaccgata tagcaatcca gttcaaatcg
     7501 gtgccgcaca acatctttgc cgcggacgag ccgatcctgc aggccaaccg gctcgtcatc
     7561 cacctgcagc cgcaggaaag cgtgacgctc gagatcctga gcaaacaacc gggcctggaa
     7621 ggagtgcgcc ttcgcgaagt cccgctcaac ctctcgctgc ccaaggacga gagcggcggt
     7681 cgcacgcgca tcgcctacga gcgactgctg ctcgatgcac tgaaagggaa cactacgctg
     7741 ttcgtgaggc gcgacgaggt cgaggccgcg tggcactgga tcgatggcat ccagacaagc
     7801 tggcgctcga cgcgccaact ggtgaagccg tatcctgcag gcacctgggg gcccacggcc
     7861 gcgatcgcac tcactgagcg acacggccac agctggtacg agtgattagt cttagaagaa
     7921 tggcgtctta gaagaatggc cacccagaag ctccacgaca ccatcatcga agtcaccgaa
     7981 cgcattggtg gccgctcggc cgcggcacgc ggcgaatatc tcgaacgtac gcgcaagcga
     8041 aaactcatcg agccggcgcg cggcaaactc gcctgcgcca acctcgcgca tgtcatggcc
     8101 gcttcgggcg tcgacaagcc gcagctgcag cagtcggtca ttccgaacat cggcatcgtc
     8161 acttcataca acgacatgct gtcggctcat cggccgtatg aaccctatcc cgaggtcatc
     8221 cgcgcggcgg cgcgcgtggc cggtgccacg gcgcaggtcg cgggtggcgt gcccgccatg
     8281 tgcgacggcg tcacgcaggg tcgcgcgggc atggagctgt cgctgttcag ccgcgatgtg
     8341 attgcactgg cgaccgcggt cgcgctgtcg cacgaagcct tcgacagcgc cttgttgtta
     8401 ggcatctgcg acaagatcgt gccgggcctg ttcatcggcg cggcctcgtt cggccagatg
     8461 cccatcatct tcgtgccggg cggcccgatg ccgagcggca tggccaacaa ggccaaggcc
     8521 gaagtgcgcc agcgatacgc agaaggcctg gccacgcgcg atgaactgct ggcggcggaa
     8581 atgggctcgt accacgacgc tggcacctgc acgttctacg gcaccgccaa ctccaaccag
     8641 atgctcatgg agatgatggg gctgcatgtt cccggcgccg cgtttgtcgc gccgaacacg
     8701 ccgttgcggc acgcactcac cgtggccgcc acacagcgcg ttgcgcagac caccgcactg
     8761 ggatccagct acctgccgtt cgtcgaggtc atcgacgagc gttcattcgt caatgccatc
     8821 gtgggcctga tggctaccgg cggctcgacc aatcacgtca ttcatctcgt ggccatggct
     8881 cgcgcggttg gcctgacggt gacctgggat gacatggcgg acatctcgcg cgtcacgcca
     8941 ctgatcgcgc gcgtgtatcc gaacggtgag gcggacgtga accagtttca tgccgcgggc
     9001 ggcatgtcct tcgtggtccg ggagctgatc aacgcaggcc tcgcgcatga atcggccatg
     9061 accatctggg gaccgggact gtcggcgtat gcgaaagatc ctttcctcgt cgacaaccag
     9121 ctcgtgtggc gcgatgcgcc tgagaagtcg cacgacgaaa agatcctgcg ccccgtagcg
     9181 aatccgtttt ccgcggacgg cggcctcaag ctgctgcgcg gcaacctcgg ccggtcggtc
     9241 atcaagacat cggcggtcga tccgaagtac tgggacatca ccgcgccggc gcgcgtgttc
     9301 gaaagccagg aagccatggc ggcggcgctc aaggccggcc tcaacgaaga tttcattgcg
     9361 gtcatccgcg ggcagggccc gcgcgccaac ggaatgcccg agctgcacaa gctcattccg
     9421 gcgatgtccg gcttgttagc caaggggcgg cgtgtggcca tcgtcaccga cgggcgtatg
     9481 tcaggcgcat cgggcaaggt tcccgctgcc attcacctca cgcccgaagg cgcggccgac
     9541 ggccccattt cgcggctgcg cgacggcgac atcatccgcc tggacgctcg tgccggcgtg
     9601 ctcgaggctc aggtcgacgc ggccacgttg caggcacgcg aagcggtgaa gcccgaggtc
     9661 gatacgaccc acggcgtggg ccgcgaactg ttcacgcgac tgcgcgaagg cgccagcgcg
     9721 gcggacgagg gcgcctcgat cctctagaca gggtgccggg ccgggaaacc gggaaaactc
     9781 gatctgtaat tttggggcct tggttttccc ggtttcccgg cccggcaccg ttgtaatatt
     9841 ggccgctcac ggaaagggcg accgaaaaat gaaaacgcgc gcagccatcg gctggaaagc
     9901 cggccaacca ctcgaagtcg tgacgctgga cctggcgggt cccaagaccg gtgaagtgct
     9961 ggtcgaaatc aaggcgtccg gcgtctgtca taccgatgaa ttcacgcgct cgggcggcga
    10021 tccggaaggc ttgttcccgg tggtgttcgg ccacgagggc gcgggtgtcg tcgttgacgt
    10081 tggccccggc gtcaccacgc tcaagaaggg cgaccacgtc atcccgctgt acacaccgga
    10141 atgccggcaa tgcaaatcct gcacctcgca caagaccaac ttgtgcaccg cgatccgcgc
    10201 cacgcagggc cagggcgtga tgcccgacgg caccagccgt ttctccatcg gcaacgagaa
    10261 ggtgcaccac tacatgggct gcagcacgtt ctctaactac accgtgctgc cggaaattgc
    10321 gctagccaag atccgcgagg acgcgccttt cgagaaggtg tgctacatcg gttgcggcgt
    10381 caccacgggc atcggtgcgg tcataaacac cgcgaaagtc gagcccggcg cgaacgtcgt
    10441 ggtgttcggc ttgggcggca tcggcctcaa cgtcatccag ggcgcgcgca tggcgggcgc
    10501 caacaagatc atcggcgtag acatcaaccc gaaacgcaag gcgatggccg agaagttcgg
    10561 catgacgcac ttcgtgaatc ccaaagaggt gcagggcgat ctcgttcctt acctcgtgtc
    10621 actcaccgac ggaggcgctg actattcctt cgagtgcgtg ggccacgtcg acctcatgcg
    10681 ccaggcgctc gagtgcacgc atcgcggctg gggcgtgtcg gtgattatcg gggtcgccgg
    10741 gtccggccag gaaatcaaga cgcggccatt ccagctcgtg acgggtcgcg tatggaaggg
    10801 cactgccttt ggtggcgcgc gcggccgcac cgacgtgccg aagatcgtcg actggtacat
    10861 ggacaagaag atcaacatcg acgatctcat cacgcatgtg atgccgatcg agaagatcaa
    10921 tgacgccttc gatctcatgc acaagggtga atccatccgt tcggtcgtca ctttctaact
    10981 acgaggaaag ctccatggcc atgaaattac atcc
//
LOCUS       DASQJS010000308        13022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3334400, whole genome shotgun sequence.
ACCESSION   DASQJS010000308 DASQJS010000000
VERSION     DASQJS010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13022
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3334400"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VEW08_12390"
     CDS             complement(<1..606)
                     /locus_tag="VEW08_12390"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HYJ41584.1"
                     /translation="MASLKDILVVVEGGAVEGVVTESRAIRSGEKYVTPQGFTAIKDG
                     QKQRGASAPATGRKPAWIRAQLPVGSGFGAVKGIVTEHRLATVCEEAKCPNIGECWNA
                     GTATIMLMGAVCTRACRFCSVDTGNPRGWLDAEEPENTARSVELMKLKYIVLTSVNRD
                     DLPDGGAEHYAAAIRAIKRRTPHVAVEALTPDFQGVLRDVET"
     gene            complement(624..2135)
                     /locus_tag="VEW08_12395"
     CDS             complement(624..2135)
                     /locus_tag="VEW08_12395"
                     /inference="COORDINATES: protein motif:HMM:NF017870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HYJ41585.1"
                     /translation="MKKIVIAVVVLVVVLLVAAPWGVGKFAEKRLDYGLEKLVEAAPY
                     LTVVERKYTQGWFKSEHVVTFEVFSSWMKAMSPKAMEDAMGSGGVESEDADAEAPVAP
                     SDAPATAEESTAPDEAPNPNEMMRFTVRNEVLHGPVLGLSGFGIARVNSHFVLSDKVR
                     KEMEEIFGPKDPVEISTRLGFLGGGTTTFKSEGRTIKPKDSKAEISWETFKLAIGYSK
                     NADKYDIDGKWPKFEVNDRGEKTKIVMTDMTIDGDGKRVRGDLYDGDLNFGIRQFTFV
                     GNDGEAVEVGDFHYIVNSETKGDFTGMSARMGLGEVKSKELTAQGIVIEEAHYDVGVR
                     HLHAPTLEKIVAEFKAMYARPLANTLEASDIMFAPLKEHGMELLKYNPEFVIDRIGIV
                     TPDGDGYIKGLVKLKGATADDFGSGSMGLIGKIDADITIDVSEKMIQKFPNGSTAAGA
                     AVDSGYVKREGERLICKIVFAGGQLTVNGKPQAIPGLGGPPSGEGEMPPSPQE"
     gene            complement(2159..2815)
                     /locus_tag="VEW08_12400"
     CDS             complement(2159..2815)
                     /locus_tag="VEW08_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYJ41586.1"
                     /translation="MVLAAGRGERMRPLTDREPKPLLRVGGKRLIEYHLERLAAGGFR
                     EVVVNTAWLGDMIEAVLGSGRQYGLAITYSHERPEALETGGGIFHALPLLGSAPFLLV
                     NGDVWTDIDFGALRRSPPEHSLAHLVLVPNPPQHARGDFLLEHGLVSDGEGPRLTYSG
                     IGIYRPELFSGCEPGKFPLLPLLRQAIARRALSGEMHQGRWYDIGTPERMAALDAQLS
                     "
     gene            complement(2821..4110)
                     /locus_tag="VEW08_12405"
     CDS             complement(2821..4110)
                     /locus_tag="VEW08_12405"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HYJ41587.1"
                     /translation="MLKNDARLSLIRSWLTRDLEWRIGRISVASADASFRRYFRVSRG
                     DVDPAAWAPKADTLIVMDAPPGKEDIAPYLKVTSLLEQAGAHVPHVHASDGKRGFIVM
                     EDLGDTQYLSLLKTGRGVDKLYGEALTTLANIQVRGLRAAATLDAYDRAPLERELNLM
                     PEWFLGKHLRLELSPEERALLTVTNEFLINEALLQPQVFVHRDFHSRNLMVLPQSKTD
                     NRGPGVIDFQDALRGPIGYDLVSLLKDCYISWSRERVERWVRGYRRVLGNLGANIGDS
                     EYQFMRWFDVIGVQRHVKVLGIFARLWHRDGKIGYLADLPLVLNYVLDACRRYPELVE
                     FGRWLEWRVAPELPLANARQKEKPAHRKPKPKPKPKPKPKPKAKAKAKAKVRRRKPAK
                     AKSARVVRKPRKKVHARKKVRARKPAQRVRRKRRQHK"
     gene            4132..6363
                     /locus_tag="VEW08_12410"
     CDS             4132..6363
                     /locus_tag="VEW08_12410"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HYJ41588.1"
                     /translation="MARETSTATLLMLAIASFSPAVVHADETADGDDTVCPEPASADG
                     TPPPAPGTEPVDDRVTIEADDKDFSFDANGNAVVSGNVVMRQGDKVIKADRLEYDAKN
                     GRAKLTGAVEFSGPAFKVRGSSGAYSPALGAQFEGAQFELPQSNARGAARNMQVDANG
                     TLTLEDVSFTTCPLTDRAWEIKSRRIVIDSRANTGTGHGTRVEFKDVPFVYLPWMTFP
                     VGPQRKSGFFFPKIGGGSRNGAEISVPYYWNIRPNADLMAAPVYYAKRGVDFAGELRY
                     LTRRQRGTLDFNYLPNDDIEQIDRHRIQLEHVAELPGDWRFRIDATDVGDSRYFEDFA
                     HGPEGTSVPFTERLAEATFRDEHWNVRAQIQDFQTIDEDLPVDDRPYARTPRVLASGD
                     WDLGLGAIDYGFDGEIVNFERNVGVTGWRMDVAPRVGFDWSAPGFFVRPSGGFRYTQY
                     SLENNDPGTDDSPSRSLPFASLDAGLVFERAAGSRGQRRMTLEPRALYLYAPFREQSE
                     LPLFDTGLPDLNLVQLFRANRYVGADRVNDANQVSFGLTSRLFDADTGAQYLAASFGQ
                     AYYFEKPRVVLPDEPVATRDTSDFIAQVSLSAYKNWNVEAGLQWNPEDTRSERSQFRL
                     QYRPAGDRVLNLSYRTQRDRLEQAELSGAWPIGKHWNAYARGVYSLRDSQMLERFAGF
                     EYKACCWRLRAVARRSVSNREGTQETSFYLQLELKGLASVGDADAFLEHTIRGYSPET
                     SAE"
     gene            6375..7685
                     /locus_tag="VEW08_12415"
     CDS             6375..7685
                     /locus_tag="VEW08_12415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYJ41589.1"
                     /translation="MISKDFSRGFLIVLACFAAPAAFSQTREASSSGVLLDRVAATVN
                     EGVVLSSELDEQMLVISERLRAQKHELPPQNVLRQQVLDRLVLQELQMQRASRAGIKV
                     SDETLNNALRDVAEQNKIPLTDLPAALASQGIDYASYREGIRKELAMQILRQRDVIGR
                     INVSPREIDQFLERQKKMPSESNEYHVSHILVAVPQAATPEELDESAKRAEEVYTRAT
                     AGEDFGQLAVSFSNAQTALEGGDLGWRKGPELPTFLAEVIASMKQGDITKPMRTPSGF
                     HLVKLNEVRGNAQVIVNQVHARHILIKPNELQDDATVKQKLDGIRDRILKGENFAATA
                     SVVSEDPGSSAEGGDLGWAGPGTFVPEFEKQLAQLQPDEISQPFRTQFGWHIIQLLGR
                     RQFDTTDDVRRQRAFTALREAKADEETELWLRRLRDEAYVEYKL"
     gene            7682..8692
                     /gene="pdxA"
                     /locus_tag="VEW08_12420"
     CDS             7682..8692
                     /gene="pdxA"
                     /locus_tag="VEW08_12420"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HYJ41590.1"
                     /translation="MSAAHKPIAVTAGEPAGIGPDLCLALAGLDLPSTFVVLGSRTLL
                     AERARMLGLNIELDAYLPGAPPRGPQSGRLQVLDIPLAAPCVAGRLDIANARHVLALL
                     DRATDGCISGEFAAMVTAPVQKSVINDAGIPFTGHTEYLAARTGATQPVMLLAADSLR
                     VALATTHLPLRAVSDAITATLLDTTLRILEDDVRRLWGISRPRVAVCGLNPHAGESGH
                     LGTEDRDVIQPAIERARARGMMVDGPIPADTVFVPRALSNYDVVLAMFHDQGLPVLKH
                     AGFGHAVNVTLGLPIVRTSVDHGTALDLAGTGRADAGSLIAATHLALEFAKRVPGRIG
                     VQ"
     gene            8711..9556
                     /gene="rsmA"
                     /locus_tag="VEW08_12425"
     CDS             8711..9556
                     /gene="rsmA"
                     /locus_tag="VEW08_12425"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HYJ41591.1"
                     /translation="MARDARSYPGFPARHRFVIFARKRFGQHFLHDRSVLDRIVREIA
                     PTPSQALLEIGPGRGALTERLIGLARTLDAIEIDRDLAAQLRARWGAPQSFELHVADA
                     LEFDFAALARMRGDRLRVIGNLPYNISTPLLFHVAAAHEHIDDLHVMLQKEVIDRIAA
                     APGSESYGRLTVMLAPWFEAKHLFDVGPGAFTPAPRVWSAVARLTVRREPAFVVPPAF
                     ARTVSAAFSQRRKTLRNGLRSLLGAEGFGAADIDPSRRPETLSPAEFARLAAQVPEDP
                     SSRPS"
     gene            9586..9966
                     /gene="apaG"
                     /locus_tag="VEW08_12430"
     CDS             9586..9966
                     /gene="apaG"
                     /locus_tag="VEW08_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HYJ41592.1"
                     /translation="MEQAQHKIRVDVDTSYLEEQSDPKERRYVFSYTITIRNEGSVPA
                     RLLARHWIITDSNGKVQEVRGDGVVGEQPYLKPGQGFRYSSGAVLETPVGAMQGSYQM
                     VGDDGEQFDAPIAAFRLAMPGLLH"
     gene            9981..10820
                     /locus_tag="VEW08_12435"
     CDS             9981..10820
                     /locus_tag="VEW08_12435"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HYJ41593.1"
                     /translation="MARIAIGDVQGCCDELKALLARCKFSADRDQLWFVGDLVNRGPK
                     SLETLRFVRALGANATVVLGNHDLHLLALSFGSKRKPKDSDTLAEVLAAPDRDQLLEW
                     LLGRPLAVFDEPRGDFLVHAGLVPEWTPRVAAKLAREVEAVLRDDARTLFDAMYGNKP
                     DKWSDSLRGMDRLRFVINAFTRMRFCHGDGTVDLKAKGAPGEQPEGLHPWFDVPGRKS
                     SEVRVICGHWSTLGFKRRRDLLALDTGCVWGGALTAVDLDADEPPIQLNCESHQEPGG
                     GEG"
     gene            complement(10793..11392)
                     /locus_tag="VEW08_12440"
     CDS             complement(10793..11392)
                     /locus_tag="VEW08_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41594.1"
                     /translation="MKFRLASGLSLIVACSLGLSACSSIKMPHVWPFYKKPKPVPVAV
                     LELNLVNADGSAASFPQYWKRNTLVIDLSGAGGTGTFAARLPEETTWPVRLAVRVRPG
                     SVGQVEIQGEERSVLPVSNEGTANIDLDVVPSVYTPKTAAIYISWGPIPAFAEAAPVE
                     ATPAFVSPTTVPAESTPSAGEIIAPAEAQPAQPSPPPGS"
     gene            11542..12057
                     /locus_tag="VEW08_12445"
     CDS             11542..12057
                     /locus_tag="VEW08_12445"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HYJ41595.1"
                     /translation="MRELKAFNFKRWIDEHRHLLKPPVGNKLVFQDSEFIIMVVRGPN
                     SRKDFHVDPGEEFFYQLEGGITLATVQHGKRVDVPIGEGEIFLLPPDMPHSPRRPAHS
                     IGLVIERTRRPGELDGFQWYCENCGTKLYEEFVEVTNIETQLPPVFDRFFGSLAHRTC
                     TQCGTVMDKPK"
     gene            12062..12877
                     /locus_tag="VEW08_12450"
     CDS             12062..12877
                     /locus_tag="VEW08_12450"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HYJ41596.1"
                     /translation="MFAELNFGGQKVRAALDRGVSLAIAVDFSGAGPRHFGAGAPSAK
                     PFAVGNFSGSVATGASANCSSITLIPHCQMTHTESVAHLTRESGDAWRVVPRGLLPAV
                     VVSVIPEPARESNESTDPQPWGTDNLITRRRLRAAWPLGKMIDPVAAVIRTLPNDTAK
                     RTRDYSDLVPPYLTREAVEWLVEKRIEHLVLDVPSVDRTHDEGHLVGHRIFFGLPPGS
                     TARGDASRSRATITELAFIPDEVADGPCILTLAVPAIGGDAVPSQPIVYPLAP"
BASE COUNT         2244 a         4409 c         4062 g         2307 t
ORIGIN      
        1 ggtttcgaca tcgcgcaaca cgccctggaa atccggcgtc agtgcttcga cagcaacgtg
       61 cggcgtgcgc cgcttgatgg cgcggatcgc cgcggcgtag tgctccgcgc cgccatcggg
      121 cagatcgtcc cggttcaccg acgtgagcac gatgtacttg agcttcatga gctcgacgct
      181 gcgcgcggtg ttctcgggtt cttcggcgtc gagccagcct cgcgggttgc cggtgtccac
      241 cgagcagaag cggcaggctc gcgtgcatac cgctcccatc aacatgatgg tggcggtgcc
      301 ggcgttccag cattcaccga tgttcgggca cttggcttcc tcgcaaacgg tcgcgagccg
      361 gtgctcggtc acgatcccct tcaccgcgcc gaagccggag cccaccggca actgcgcgcg
      421 gatccacgcc ggcttgcgtc ccgtcgccgg agcgctcgcg ccacgctgtt tctgcccgtc
      481 cttgatggcc gtgaagccct gcggagtcac gtatttctcg ccactgcgaa tcgcgcgcga
      541 ttcggtgacg acgccctcca cggcgccccc ttccacgacg accaatatgt ccttgagtga
      601 tgccatgtct gaaggcgcgg attctactcc tgcggcgacg gcggcatctc tccctcgccc
      661 gatggaggtc cgccaagtcc cggaattgcc tggggtttgc cgttcaccgt gagctggccc
      721 ccggcaaaga cgatcttgca gatcaggcgt tcgccctcgc gcttgacgta acccgaatcg
      781 actgccgcac ccgcagccgt ggagccattg gggaatttct ggatcatctt ttccgacaca
      841 tcgatcgtga tgtcggcgtc gatcttgccg atgagcccca tgctgccgct gccgaagtca
      901 tcggcggtcg cgcccttcag cttgaccaga cctttgatgt agccgtcgcc gtcgggggtc
      961 acgatgccga tgcgatcgat gacgaactcg gggttgtact tgagcagctc catgccgtgc
     1021 tccttgagcg gcgcgaacat gatgtcgctg gcttccagcg tgttggccag cggcctggcg
     1081 tacatggcct tgaactccgc cacgatcttt tccagagtcg gcgcgtgcag gtgccgcacg
     1141 ccgacgtcgt agtgggcttc ctcgatcacg atgccttgcg cggtgagttc cttcgacttc
     1201 acctcaccca accccatcct ggcgctcatg ccagtgaagt cgcccttggt ctcactgttc
     1261 acgatgtagt gaaagtcgcc gacttcgact gcttcgccgt cgttgccgac gaaggtgaac
     1321 tgcctgatcc cgaagttgag gtcgccgtcg tacagatcgc cgcgaacgcg cttgccatcg
     1381 ccatcgatcg tcatgtccgt catgacaatt tttgtttttt cgccgcggtc gttgacctcg
     1441 aacttcggcc acttgccgtc gatgtcatat ttgtcggcgt tcttcgagta accgatggcg
     1501 agcttgaagg tttcccagct gatctccgcc ttggaatcct tcggcttgat cgtgcggccc
     1561 tcggacttga aggtggtggt gccaccgccc aggaatccga ggcgcgtgct gatttcgacc
     1621 ggatccttcg ggccgaagat ctcttccatc tccttgcgga ccttgtcgct cagcacgaag
     1681 tgcgaattca cgcgcgcgat gccgaagccg gacaggccga gcaccgggcc atgcaggact
     1741 tcgttgcgca cggtgaaacg catcatttca ttggggttcg gcgcctcatc cggtgcggtg
     1801 ctctcttccg cggtggctgg cgcgtctgac ggcgcaacag gcgcttccgc gtcggcatcc
     1861 tcggactcga ctcccccgct gcccatggcg tcttccattg ccttggggct catcgccttc
     1921 atccagctgc tgaagacttc gaacgtgacc acgtgctcgg acttgaacca gccctgcgtg
     1981 tacttgcgtt cgacgacggt gagatacggc gcggcttcga cgagtttctc gagcccatag
     2041 tcgagtcgct tctcggcgaa tttgccgacg ccccatggcg ccgcaaccaa caacaccacc
     2101 acgagaacga cgaccgctat gactatcttt ttcatcttgc ctccctggga attcatgttc
     2161 acgacagctg cgcgtcaagc gcggccatcc gctccggggt gccgatgtcg taccaccggc
     2221 cctgatgcat ttcaccgctg agggcgcgcc gggcaatcgc ctggcgcagc aacggcagca
     2281 gcggaaactt tccgggctca cagcccgaaa acaattccgg gcgatagata ccgatacctg
     2341 agtacgtcag tcgcggaccc tcgccgtctg acacgagtcc atgctcgagc agaaaatcgc
     2401 cgcgggcgtg ctgcggcggg ttcggcacga ggaccagatg cgcaagcgag tgctcgggtg
     2461 gtgaacggcg cagggcgccg aaatcgatgt ccgtccacac atcgccattc accagcagga
     2521 acggtgcaga tcctaacagc ggcagcgcat gaaaaatgcc ccctcccgtc tccagggcct
     2581 ccgggcgctc gtggctgtag gttatggcaa gcccatattg ccgccccgat ccgagcaccg
     2641 cctcgatcat gtcgcctaac cacgcggtat tgacgaccac ttcccggaaa ccgccggcgg
     2701 cgagccgctc caggtgatat tcgatgagcc gttttccgcc cacgcgcagc aggggtttgg
     2761 gctcccgatc ggtcagcgga cgcatgcgct caccgcggcc ggcggccagc accattgcct
     2821 tcatttgtgc tgacgccgct tccggcgcac gcgttgcgcg ggcttgcggg cgcgtacctt
     2881 cttgcgcgcg tgaaccttct tgcgcggttt gcgaactacc cgcgcggact tcgccttcgc
     2941 gggcttgcgg cgcctgactt tggccttggc cttggctttg gctttgggtt tgggtttggg
     3001 tttgggttta ggtttaggtt tgggcttgcg gtgcgcgggc ttttctttct ggcgcgcatt
     3061 cgcaagcggc aactctggcg ccacgcgcca ctcgagccag cgtccgaact ccacgagctc
     3121 cggatagcgc cggcaggcgt cgagcacata gttcagcacc agcggcaggt cggcgagata
     3181 tccgatcttg ccgtcgcgat gccaaagccg cgcaaagatg cccagcacct tgacgtgccg
     3241 ctgcacgccg atgacatcga accagcgcat gaactggtat tcgctgtcgc cgatgttcgc
     3301 gccgaggttt ccaagcacgc ggcgatagcc gcgcacccag cgctcgacac gttcgcgcga
     3361 ccagctgatg tagcagtcct tgagcagcga cacgaggtcg tagccgatcg gaccccgcag
     3421 cgcatcctgg aagtcgatga cgccgggacc ccgattgtcc gttttggact ggggaagcac
     3481 catgaggttg cgcgagtgga agtcgcgatg aacgaaaacc tgcggctgca gcagcgcttc
     3541 attgatgagg aactcgttgg tcaccgtcag cagcgcgcgc tcctcgggtg agagttcgag
     3601 ccgcagatgt ttgcccagga accactcggg catgagattg agctcgcgtt cgagcggtgc
     3661 gcggtcgtag gcatcgagag tcgcggccgc gcgcaggcct cgcacctgga tgttggcgag
     3721 cgtggtcagc gcctcgccgt agagtttgtc gacgccacgg cccgtcttga gaagcgagag
     3781 gtactgggtg tcgcccaggt cttccatcac gatgaatccg cgcttgccgt ccgaggcgtg
     3841 gacgtgcggc acgtgcgcac cggcctgctc cagcagtgaa gtcaccttga ggtagggagc
     3901 gatatcttcc ttgccgggcg gcgcatccat gacgatgagg gtgtcggcct tcggcgccca
     3961 ggcggccgga tcgacgtcgc ctcgcgacac gcggaaatag cggcggaagc tggcgtcagc
     4021 ggatgcgacg ctgatacggc cgatgcgcca ttccaggtcg cgtgtcagcc aggaccggat
     4081 gagtgagagt cgcgcgtcgt tcttgagcat tcgtataagt atagtgcgca aatggcgaga
     4141 gagacttcga ccgcaacgct gctgatgttg gcaatagcca gcttttcacc cgccgttgtc
     4201 catgccgacg agacggccga tggcgacgac acggtttgcc ctgaacccgc gtccgccgac
     4261 ggtaccccgc cgcctgcccc cggcacggaa ccggtcgacg atcgggtgac catcgaggcc
     4321 gacgacaagg acttcagttt cgacgccaac ggcaacgccg tggtgtcggg caacgtcgtg
     4381 atgcgccagg gcgacaaggt catcaaggcc gatcgcctgg aatacgacgc caagaacggg
     4441 cgcgccaagc tcacgggtgc ggtcgagttt tccggtccgg cattcaaggt acgcggcagc
     4501 agcggcgcct attccccggc tctcggcgcg caattcgaag gagcgcagtt cgagctgccg
     4561 cagagcaatg cccgcggcgc cgcgcgcaac atgcaggtgg atgcgaacgg cacgctgacg
     4621 ctggaagacg tctcgttcac cacctgcccg ctcaccgacc gcgcgtggga gatcaagtcc
     4681 cgacgcatcg tgatcgactc gcgcgcgaat accggcaccg ggcacggcac gcgagtcgag
     4741 ttcaaggacg tgccgttcgt ctatctaccc tggatgacct tccccgtcgg cccgcagcgc
     4801 aagagcggct tcttcttccc gaaaatcggc ggcgggtcgc gcaatggcgc ggagatctcg
     4861 gtgccctact actggaacat ccggccgaac gctgacctca tggccgcacc ggtctattac
     4921 gccaagcgcg gcgtggattt cgccggcgaa ctgcgctacc tcacgcgacg tcagcgtggc
     4981 acgctggact tcaactatct gcccaatgat gacatcgagc agatcgaccg gcatcgcata
     5041 caactcgaac acgtcgccga actgcccggc gactggcggt tccgcatcga cgccacggac
     5101 gtcggcgaca gcaggtactt cgaggatttc gcgcacggac ccgagggcac cagcgtgccc
     5161 ttcacggaac gcctggcgga agccacgttt cgcgacgagc actggaacgt gcgcgcacag
     5221 atacaggact tccagaccat cgatgaagac ctgccggtcg atgatcgacc ttacgcgcgc
     5281 acgccgcgcg tcctcgcctc cggcgactgg gacctggggc tgggcgccat cgactacggg
     5341 ttcgatggcg agatcgtgaa tttcgagcgc aatgtcggcg tcaccggctg gcgcatggat
     5401 gtggcgccac gcgtcggctt cgactggtcg gctcccgggt ttttcgtgcg tccgtccggt
     5461 ggctttcgct acacgcagta ctcgctcgag aacaacgacc cggggactga cgattcgccc
     5521 tcgcgctccc tgccattcgc gtcgctcgat gccggcctcg tgttcgaacg cgcggccggt
     5581 tcacgtggcc agcgccgcat gacactcgaa ccgcgcgcgc tttatcttta cgcgcccttt
     5641 cgtgaacaga gcgaactgcc gttgttcgac accgggttgc cggacctgaa cctggtgcag
     5701 ctctttcgcg ccaatcgtta tgtgggtgcc gaccgggtca acgacgccaa ccaggtctcg
     5761 ttcggcctca cctcccgcct cttcgatgcc gacacgggcg cgcaatatct cgcggccagc
     5821 ttcggccagg cgtactactt cgagaaaccg cgcgtcgtgc tgccggatga gccggtggcg
     5881 acgcgcgaca catcagactt catcgcgcag gtgtccctgt cggcgtacaa gaactggaac
     5941 gtggaagccg ggctgcaatg gaatcccgaa gacacgcgca gcgagcgctc acagttccgc
     6001 ctgcaatacc ggccagccgg cgatcgcgtg ttgaacctgt cctaccgcac tcaacgcgat
     6061 cgcctcgaac aggccgaatt gtcgggcgcc tggccgatcg gcaagcattg gaacgcctac
     6121 gcccgcggtg tctacagctt gcgcgacagc cagatgctcg agcgctttgc cggcttcgaa
     6181 tacaaagcgt gttgctggcg cctgcgcgcc gtggcgcgcc gctcggtctc caatcgtgag
     6241 ggcacccagg agaccagctt ctacctgcag ctggaactga agggcctcgc cagtgtcgga
     6301 gatgcggacg ctttcctgga acacacaatt cgaggatact cgccggaaac ttcggccgag
     6361 tgaaaaccaa ctacatgatt tcaaaagact tttcgcgcgg cttcctgatt gtcctggcct
     6421 gtttcgctgc tccggcggcg ttctcccaga cccgcgaagc cagcagttcc ggtgtgctcc
     6481 tcgaccgcgt cgcggctacc gtgaacgagg gcgtcgtgct gtcgagcgag ctcgacgaac
     6541 agatgctggt catctccgaa cgcctgcgcg cgcagaaaca cgagctgccg ccgcaaaacg
     6601 tcctgcgcca gcaggtactc gaccgcctgg tcctgcaaga gctgcagatg cagcgcgcca
     6661 gccgcgccgg catcaaggtg tccgacgaga cgctgaacaa cgcgctgcgc gatgtggccg
     6721 agcagaacaa gattccactg accgacctgc cggccgccct ggccagccag ggcatcgact
     6781 acgccagtta tcgcgaaggc atccgcaagg aactcgcgat gcagatcctg cgccagcgcg
     6841 atgtcatcgg ccgcatcaat gtcagcccgc gcgaaatcga ccagttcctc gagcggcaga
     6901 agaagatgcc gtccgagtcg aacgagtacc acgtctcgca catcctcgtg gccgtgccgc
     6961 aggcggctac gcctgaagag ctcgatgaat ccgccaagcg cgccgaagag gtctacacgc
     7021 gcgctaccgc cggcgaagac tttggacagc tggctgtctc gttctccaac gcccagaccg
     7081 cgctcgaagg cggcgatctc ggctggcgca agggcccgga gctgcccaca ttcctcgccg
     7141 aggtcatcgc cagcatgaag cagggcgaca tcaccaagcc gatgcgcacg ccatcgggct
     7201 tccatctcgt caagctcaac gaggtgcgcg gcaacgccca ggtcatcgtc aaccaggtgc
     7261 acgcgcgtca catcctcatc aagcctaacg aactgcagga cgacgccacc gtgaagcaga
     7321 agctcgatgg gatccgcgac cgcatcctca agggcgagaa cttcgccgcc accgcctcgg
     7381 tggtctcgga agaccccggc tcctcggccg agggcggcga tctcggctgg gccggccccg
     7441 gcaccttcgt cccggagttc gaaaagcagc tcgcgcaatt gcagccggat gaaatcagcc
     7501 agccgttccg aacgcagttc ggctggcaca tcatccagtt gttaggccgc cgccagttcg
     7561 acaccaccga cgacgtgcgc cgccagcgcg ccttcacggc gctgcgcgaa gccaaggccg
     7621 acgaggaaac ggaactctgg ctgcggcgcc tgcgcgacga ggcgtacgtc gagtacaaac
     7681 tctgagcgca gctcacaagc ccattgccgt caccgcgggc gaacccgccg gcatcgggcc
     7741 ggatctgtgt ctcgccctcg ccggtctcga cctcccttcc acattcgtag ttctcggcag
     7801 ccgcacgctg ctggcagagc gtgcgcgcat gctcggcctg aacatcgagc tcgatgccta
     7861 tctacccggc gccccgccgc gcggcccgca gtcgggccgg ctgcaggtgc tcgacatacc
     7921 gctggcggcg ccctgcgtgg ccggccggct cgatatcgcc aacgctcggc acgtgctcgc
     7981 cctgctcgac cgcgccaccg acggctgcat cagcggcgag ttcgcggcca tggtcacggc
     8041 gccggtccag aagagcgtca tcaacgatgc cggcatcccg ttcaccgggc acaccgaata
     8101 cctcgcggcg cgcaccggcg caacgcagcc ggtcatgctg ctcgccgccg actcgcttcg
     8161 cgtggcgctc gccacgacgc acctgccatt gcgcgcggtc agcgatgcga tcacggcgac
     8221 gctgctcgac acgacgctgc gcatcctcga agacgacgtg cgacggctct ggggcatctc
     8281 gcggccgcgc gtggccgtgt gtgggctcaa tccgcacgcc ggcgagagcg ggcacctcgg
     8341 caccgaagac cgcgacgtga tccagcctgc catcgaacgc gcccgcgccc gcgggatgat
     8401 ggtggatgga ccgatcccgg ccgacacggt gttcgtaccg cgcgcgctgt ctaactacga
     8461 tgtggtgctg gcgatgtttc acgaccaggg actgccggtg ctcaaacacg ccggcttcgg
     8521 ccacgccgtg aacgtgacgc tgggactgcc catcgtgcgc acctccgtgg accatggcac
     8581 ggcgctcgat ctcgccggca ccggccgtgc cgacgccggc agcctcatcg ccgcaaccca
     8641 cctcgcgctc gagttcgcga agcgggtgcc gggccggatt ggagtccagt agtgcgacga
     8701 agtgggttcg gtggcacgag atgcgcgtag ttatcccggt ttcccggccc ggcaccgctt
     8761 cgtgatcttc gcgcgcaagc gtttcggcca acatttcctg catgaccgca gcgtgctcga
     8821 tcgcatcgtg cgcgaaattg cgccgacccc ctcgcaggcg ttgctggaga tcgggccggg
     8881 gcgcggcgcg ctcaccgagc gcctgattgg cctggcccgc accctggatg ccatcgagat
     8941 cgatcgggac ctcgccgcgc agctgcgcgc gcgctggggc gcgccccaat cgttcgagct
     9001 ccacgtcgcc gacgcactcg aattcgactt cgcggcgctg gcgcgcatgc gcggcgaccg
     9061 gctgcgcgtc atcggcaacc tgccctacaa catctccacg ccgctgctgt ttcacgtcgc
     9121 cgccgcccac gaacacatcg acgacctgca cgtgatgctg caaaaagagg tcatcgaccg
     9181 catcgcggcc gcgcccggca gcgagtccta cggccggctc acggtcatgc tcgcaccctg
     9241 gttcgaggcc aaacatctgt tcgatgtcgg tccgggcgcg ttcacgcccg cgccccgcgt
     9301 ctggtccgcc gtggcgcgcc tcaccgttcg ccgggagccg gcattcgtag tgccccccgc
     9361 gtttgcgaga acggtgtcgg cggccttctc ccaacgccgt aagacgttac gcaatggctt
     9421 gcgttctctg ctaggcgccg aaggttttgg cgccgcggac atcgatccct cccgtcggcc
     9481 cgaaacgctt tcgcccgcgg agttcgccag attggcagcg caggttcccg aagatccttc
     9541 atcgcggcct tcatagcgaa catagttggg tatcatctga ccgacatgga gcaagcccaa
     9601 cacaagatcc gcgtcgacgt cgacacctcg tatctcgaag aacaatcgga tccgaaggaa
     9661 cggcgctacg tcttctcgta caccatcacc atccgcaacg aaggcagcgt gcccgcacgc
     9721 ctgctcgcgc ggcactggat catcaccgac tccaacggca aggtgcagga agtccgcggc
     9781 gacggcgtgg tgggtgaaca gccctacctc aaacccggcc aggggtttcg ttattccagc
     9841 ggcgccgtgc tcgaaacacc ggtcggcgcc atgcagggca gctaccagat ggtgggcgac
     9901 gacggagaac agttcgacgc gcccatcgcc gcgttccggt tggcgatgcc cggcctgctg
     9961 cattgaaacg tgtgcgctga atggcacgca tcgccatcgg tgacgtccag ggctgttgcg
    10021 acgaactgaa ggcgctgctg gcgcgctgca aattctccgc cgatcgcgac cagctctggt
    10081 tcgtgggcga cctcgtcaac cgcggcccga agtccctgga aacattgcgt ttcgtgcgcg
    10141 cgctcggcgc caacgccacc gtggtcctcg gcaaccacga cctgcatctg ctggcgctca
    10201 gtttcggatc gaagcgcaag cccaaggaca gcgacacgct ggccgaggtg ctggccgctc
    10261 cggaccgcga ccagctcctc gaatggttgt taggccggcc actggcggta ttcgacgagc
    10321 ctcgcggcga cttcctggtg cacgcggggc tggtcccgga atggacaccc agggttgccg
    10381 ccaagctcgc gcgggaagtc gaagcggtgc tgcgtgacga tgcgcgcacg ctgttcgacg
    10441 ccatgtacgg caacaagccg gacaaatgga gcgattccct gcgaggcatg gaccggctgc
    10501 gtttcgtcat caatgccttc acgcgcatgc gcttctgcca cggcgacggc accgtggatc
    10561 tcaaggcgaa gggtgccccg ggcgaacagc ccgaggggct gcatccctgg ttcgacgtgc
    10621 cgggacgcaa gagcagcgag gtgcgcgtga tctgcgggca ttggtcgacg ctgggcttca
    10681 agcgccgccg tgacctgctg gcactggata ccgggtgcgt gtggggcggc gcgctgaccg
    10741 cggtcgacct ggacgcggac gaaccgccga tccagctaaa ttgcgagagc catcaggagc
    10801 cgggcggcgg cgagggctga gccggctgcg cctcggcggg tgcaatgatt tcgccagcgc
    10861 tgggtgtgct ttccgccggt actgtcgtcg gggacacgaa tgcgggggtg gcttccaccg
    10921 gcgccgcctc ggcgaatgcc ggtatcggcc cccaggaaat gtagatagct gccgtcttcg
    10981 gcgtgtaaac gctgggcacc acgtcgaggt cgatgttcgc ggtgccctcg ttgctgaccg
    11041 gcagcacgct gcgttcttcg ccctggattt cgacctgtcc gacgctgccc ggacgcaccc
    11101 gcacggcgag ccggaccggc cacgtggttt cttcgggaag ccgggccgcg aaagtgccgg
    11161 ttccgccggc gcctgacagg tcgataacca gcgtattgcg cttccagtac tgcgggaagc
    11221 tcgcggccga gccatccgcg ttcaccaaat taagctcgag taccgccacc ggaaccggct
    11281 tgggcttctt atagaaaggc cagacgtgcg gcatcttgat cgagctgcag gccgacaggc
    11341 ccagggagca ggcaacgatc aacgacaggc cggacgcaag gcggaatttc acggcagacc
    11401 actccacgag ctgttagtgg gcggaaacat aacacggggc ctcccgcccg aggctcgcct
    11461 gcctgacgcc gaccggcgac agcgtgggca tttgtggagg gggtcacatc atgggcccgg
    11521 cagcgaatac catacgagag tatgcgtgag cttaaggcct tcaatttcaa gcgctggatc
    11581 gacgagcacc gccatctgct caagccgccc gtgggcaaca agctggtgtt ccaggacagc
    11641 gagttcatca tcatggtcgt gcgtggaccg aacagccgca aggatttcca cgtcgacccg
    11701 ggcgaggaat tcttctacca gctcgagggc ggcatcacgc tcgccaccgt ccagcacggc
    11761 aaacgagtgg acgtgccgat cggcgaaggc gagattttcc tgttgccgcc ggacatgccg
    11821 cactcgccac gtcgccccgc gcactccatt ggcctcgtca tcgagcgcac gcgccggccg
    11881 ggcgagctcg acggcttcca gtggtactgc gagaactgcg gcacgaagct ctacgaagaa
    11941 ttcgtcgagg tcaccaacat cgaaacccag ctcccgcccg ttttcgatcg cttcttcggc
    12001 agcctcgcgc accgcacctg cacccagtgc ggcacggtca tggacaagcc gaagtagccg
    12061 catgtttgcc gaactcaact tcggtggaca gaaagtccgg gccgcgctcg atcgcggcgt
    12121 gagcctggcc attgccgtcg atttctccgg cgccggcccc cgtcatttcg gcgctggcgc
    12181 gccgtcggcc aagccgttcg ccgtcggtaa cttctccggc agcgttgcca ccggcgccag
    12241 cgccaattgc tcgtcgatca cgctgatccc ccattgccag atgacgcaca ccgaaagtgt
    12301 cgcgcacctc acgcgcgaat ccggcgatgc gtggcgcgtc gtgccgcgcg gcctcctgcc
    12361 cgctgtggta gttagcgtga tccccgagcc ggcgcgcgaa tcgaacgaga gcaccgaccc
    12421 gcagccctgg ggcacggata acctgatcac acgccgccgc ctgcgtgccg cctggccgct
    12481 gggcaagatg atcgaccccg tggccgccgt gatccgcacg ctgccgaatg acactgccaa
    12541 acgcacgcgt gactacagcg acctggtgcc gccatatctc acgcgcgaag cggtggaatg
    12601 gctggtggaa aagcgcatcg agcacctggt gctcgatgtt ccctcggtcg accgcacgca
    12661 cgatgaaggc catctcgtcg ggcatcggat cttcttcgga ctgccacccg gcagcacggc
    12721 acgcggcgac gcatcacgct cgcgcgccac catcaccgag ctcgccttca ttcccgatga
    12781 agtcgccgat ggaccctgca tcctcacgct cgcggtgccg gccatcggcg gcgatgccgt
    12841 gcccagccag ccaattgtgt atcctctggc gccatgaacg cgtcgccttc cgagctcgtc
    12901 aacgcggatc tgctgtcgca gttctcgatg tcgcgggact tcgcgcagga ttgcgacgcc
    12961 gcggacatcc tggcgccgta ccgcaagcaa ttcgagctgc ccctgcacgc caatggccag
    13021 cc
//
LOCUS       DASQJS010000309        11339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1127982, whole genome shotgun sequence.
ACCESSION   DASQJS010000309 DASQJS010000000
VERSION     DASQJS010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11339
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127982"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1902)
                     /gene="purL"
                     /locus_tag="VEW08_12455"
                     /gene_synonym="purI"
     CDS             complement(<1..1902)
                     /gene="purL"
                     /locus_tag="VEW08_12455"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HYJ41597.1"
                     /translation="MIELLGASAVSDFRIAKLRPALEAIYPAIDHKIGAISARFVHFV
                     DLARKLDARETTLLERLLTYGPREDAPALDSSAATTEIVVVPRFGTISPWSSKATDIA
                     QVCGLDAVRRVERGIVWRIGSTKALSHEEAMRLATPLFDRMTEAALLDRGDAARLFVR
                     EKTRALRTVSLASGRDALVTANSELGLALSDDEIDYLVKNFAALGRDPTDVELMMFAQ
                     ANSEHCRHKIFNADWIIDGERQPKSLFAMIRNTHEKHPQGVLSAYRDNAAVMEGALGK
                     RYFPNPENDIYGAIDEPIDILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAK
                     PKAGLTGFSVSHLRIPGFEQPWETPPGAPLGKPDRIVSALDIMIEGPIGGAAFNNEFG
                     RPNICGYFRTFEQAAHGDPGGRVRGYHKPIMIAGGLGNVRRNHVEKCEIPVGTKIVVL
                     GGPAMLIGLGGGAASSVNSGASSADLDFASVQRGNPEIQRRAQEVIDRCSARWSLNPI
                     LLIHDVGAGGLSNAVPEAVAHTPGRGARIDMAAIPNDEPGMSPMELWCNESQERYVLL
                     IDAARLEDFASIARRERCPFAVLGEIDDTGVLVLEDKQHGTRPVDLPLEVLLGKAPKM
                     LRDVRRVTPA"
     gene            complement(2011..2388)
                     /locus_tag="VEW08_12460"
     CDS             complement(2011..2388)
                     /locus_tag="VEW08_12460"
                     /inference="COORDINATES: protein motif:HMM:NF020426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HYJ41598.1"
                     /translation="MKGQVTAPLVGSMASSHIFKIMFVNQGKVYEVYARKVAQGRLFG
                     FIEVEELVFGERSTVVLDPGEERIKSEFSGVKCTMLPMHSILRIDEVKKQGVSKVSDF
                     AGNNVSAFPMSMYTPASDPGTKK"
     gene            2431..3729
                     /gene="tagH"
                     /locus_tag="VEW08_12465"
     CDS             2431..3729
                     /gene="tagH"
                     /locus_tag="VEW08_12465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HYJ41599.1"
                     /translation="MALRLRVISDHRHRLGDKSTFVFGVSGGSIGRSAENDWVLPDDM
                     RYVSGRHARIVYHKGRYMLQDTSSNGTYVNDQERPLGNQNPHTLSSGDVIRIGEYHVQ
                     VQIDSATDFSLDDSALYKTGSTSTRSRRVPQPKDLGASLRLENLLEASNDISSDELKP
                     VNAFGQAVSARTRTLHQTQDLPKEPTTAPELEVDSEAVARRIARLAKAATKQQQALQQ
                     PTAPAPSAPAPTYAQSAVDNTPGLQAFCRGAGIGPETLPVEAHSRMLHLAGQLLRESL
                     LGLKESTRSQQEQRNHLRVTYQKMPGDLLPSLERHSVDELIQELLKAHDSRRFDAVQW
                     LRESFAGGRRHDEAMINAMRAAFVDFMGRLDPRDLATRFERSARRKTMGNWELYGDFY
                     RSLCEMQPGTLPHIFVETFAQNYEQAARDAEGGATQHDAA"
     gene            3832..4182
                     /locus_tag="VEW08_12470"
     CDS             3832..4182
                     /locus_tag="VEW08_12470"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYJ41600.1"
                     /translation="MKQYLISVCYPAGGTQPPPDALQRIMRDVHVIQKEMQAAGVWIF
                     SGGLTPPDSATVLRHQGGDVITTDGPFIESKEQIGGITILKAPDLDAALAWGRKLARA
                     IGTPIEVRPFAEGH"
     gene            4113..5387
                     /locus_tag="VEW08_12475"
     CDS             4113..5387
                     /locus_tag="VEW08_12475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ41601.1"
                     /translation="MGPQTRARHRHSDRSAPVRGGSLSAQIETIFRREHGRAVAVLVR
                     FLGDIGLAEEAVQDAFTIALTHWPRSGIPPSPAGWIITTARNRALDHLRREASRDERH
                     RAALQLFEQETEEEHEVRDERLRLIFTCCHPAIGTEAQLALTLRLLGGLTTAEIARAF
                     LVPEATMAQRLSRAKAKIRDAGIPYRVPEGPELPARVRSVLAVVYLMFNEGYSATSGE
                     SLVRAELCDEAIRLGRLLVEMLPGEPEALGLLALMLLIDARREARVDESGGLVRLAEQ
                     DRDRWNRAEIDEGQALMRACLVRNQPGPYQIQAAINAVHSDARRASETDWRQILELHD
                     QLLALAPSPVAELNRAVVVAEIDGAQRALERIEPLALDQYHLFHAVRADLLRRLGRDA
                     DAAAAYQAAIALCDNTREKGFLQAQYDAIARN"
     gene            complement(5389..5904)
                     /locus_tag="VEW08_12480"
     CDS             complement(5389..5904)
                     /locus_tag="VEW08_12480"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HYJ41602.1"
                     /translation="MNSTDVSKHYSCTIDSPVGALSLVATDRALVALVWRREGHRGAF
                     ENAVEKPAHPVLRDTARQLREYFAGSRRVFDVELDFRGTDFQRRVWAALLTIPYGETR
                     SYRQIAEQIGNPAAVRAVGAANGRNPISIIAPCHRVVGMNGDLTGFGGGLDAKAQLLS
                     LEAPQKQLLAG"
     gene            complement(6178..6270)
                     /locus_tag="VEW08_12485"
     tRNA            complement(6178..6270)
                     /locus_tag="VEW08_12485"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6233..6235),aa:Ser,seq:cga)
     gene            6401..7105
                     /locus_tag="VEW08_12490"
     CDS             6401..7105
                     /locus_tag="VEW08_12490"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HYJ41603.1"
                     /translation="MALGIAALGLGPPVLGAEHPAPAKPKPKPAPAKPPVQRARLPKR
                     LAADFRSHIETRLPHLRSSFEAAGRDTGLNWRMLAALGYQESRWRPAAVSPRGAQGVM
                     MLMPLTATKMGVKNVFSPDENIMAGSRYLLYMKERIPKRIRDPDRTLLAMAAYNIGIG
                     HLEDARIITQMRKKNPDVWADVRANLPRLSDPVWHKRVKRGYANGRETAQFVERVSQF
                     AAILESSVPDAVVARD"
     gene            complement(7121..8476)
                     /locus_tag="VEW08_12495"
     CDS             complement(7121..8476)
                     /locus_tag="VEW08_12495"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HYJ41604.1"
                     /translation="MRKFFCALAMLAFGVTAASADKNDATPIFDGKSLTGWKTLGGGA
                     DYKVIDGAIVGSSRPGIPNSFLVTEKVYRDFIFEFDVRQDVGPTNSGVQFRSLSTPDF
                     ENGRVHGYQTDIDPSPRQWSGSIYEEAQRGWFYTGELHPPAKALYKFGEWNHYRIEAV
                     GPRLRVWINDAPAADVIDDATKEGFFGLQVHSINNAEEAGRTVTWKNLQVQTKNLKRR
                     APMGIFIRNLLPNNLDPEEKAQGWRLLWDGKTTRGWRSANAEGFPAKGWSFADGVLAV
                     QAKGGGGDIMTDEEFGAFDLQMEFKVSAGTNSGIFYLLTSPKDPVSGAPLGLEYQILD
                     DERHPDAKLGIDGNRTLASLYDIYPRAKLMTNVGIAPRIDVWQHARIVARADGTVEHW
                     LNGVKVLEFKRGSDDYRAHVAAGKFKSTPGFGEAQKGRILLQDHGDAVSFRSIKIREL
                     K"
     gene            complement(8514..9104)
                     /locus_tag="VEW08_12500"
     CDS             complement(8514..9104)
                     /locus_tag="VEW08_12500"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYJ41605.1"
                     /translation="MRSPIRLLLCMIAGAAAAGAVAAPVTYNVDPEHTYPSFEVDHFG
                     GVSVWRGKFNENKGTIVLDREAQSGTVDITIDMDSVDLGLEKLHTHLKGTDPGMLDVA
                     KYPTATYKGKLAKFKDGAPTEVQGDLTLHGVTKPVTLTIRSFKCMAHPMKKKEFCGAD
                     AVGNFSREDFGINWGKAFGFKMEVKLAIQVEAFKAD"
     gene            complement(9214..9681)
                     /gene="tadA"
                     /locus_tag="VEW08_12505"
     CDS             complement(9214..9681)
                     /gene="tadA"
                     /locus_tag="VEW08_12505"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HYJ41606.1"
                     /translation="MPLRNDIDFMRLALEAARGAEQAGEVPVGAVLVQGDAILAVAAN
                     RPIAGHDPTAHAEIEALRAGGKTLGSYRLNGTTLYVTLEPCVMCASAIVHARVARLVF
                     GAWDPKAGAAGSTTNVFTMPSMNHRVDVFGGVLMEESARMLNAFFTARRRELT"
     gene            9763..10470
                     /locus_tag="VEW08_12510"
     CDS             9763..10470
                     /locus_tag="VEW08_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ41607.1"
                     /translation="MNTAESNDPATDILSQHVGPRREGDASIIANEIDIAMSPISLYA
                     DALLERKDLDKAARQYVTSIRRAADDVALAVARLRALDRPHTPAPEAPESRKQTPAPA
                     VRSLRVLLIDDDPSLIESLRSALVDEGHKVTTANGGQAGIDAFRAASASRMPYDIVIT
                     DLAMPDVDGRQVVASLRAASPGTPIVLLTGWRHQLADRVERPLQVDRLLGKPPRIREL
                     RAALAELTGRRATDRLD"
     gene            complement(10488..>11339)
                     /locus_tag="VEW08_12515"
     CDS             complement(10488..>11339)
                     /locus_tag="VEW08_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ41608.1"
                     /translation="LALSFVLLAALTHATWNFFAKSAVASRHFVWLYSLGSVILYAPI
                     VAWLLFTERPQFTAQHWLALGATSVLHLGYSLVLQAGYRASDLSLVYPIARGTGPLLS
                     FVGAAIFLGEQPTSLSILGLVLIVAGILLVAGLTSEPHKAPRAGILYGLGTGVFIAAY
                     TVNDGYAVKVLLISPFLIDFTGNLFRMVVLAPRAWADRARVGLEARLYRKPIIIVSVL
                     GPLGYILVLFAMRMAPISHVAPARELSTLVGAWFGSRLLREDSGPWRIAGAALIVAGV
                     IALAMPA"
BASE COUNT         2148 a         3633 c         3552 g         2006 t
ORIGIN      
        1 tgccggcgtg acgcgacgca cgtcgcgcaa catcttcggc gccttgccta acaacacttc
       61 gagcggcaga tccaccgggc gcgtaccgtg ctgtttgtct tcgagcacga gcacgccggt
      121 atcgtcgatt tcaccgagca cggcgaacgg gcagcgctca cggcgcgcga tcgatgcgaa
      181 atcttcgagg cgcgcggcat cgatgagcag cacatagcgc tcctgcgatt cgttgcacca
      241 cagctccatc ggcgacatgc cgggctcgtc gttgggaatt gccgccatgt cgatgcgcgc
      301 gccgcggccc ggggtgtgcg cgaccgcttc gggcacggca ttcgacaagc caccggcgcc
      361 cacgtcgtgg atgagcagga tgggattgag agaccagcgc gccgagcagc gatcgatgac
      421 ttcctgtgcg cggcgctgga tttccgggtt gccacgctgc accgaggcga aatcgagatc
      481 ggcactggac gcaccgctgt tcaccgacga ggcggcgccg ccacccaggc cgatgagcat
      541 ggccggcccg ccgagcacca cgatcttcgt gcccaccggg atctcgcatt tctcgacgtg
      601 attgcgacgc acgttgccga gtccacccgc aatcatgatg ggcttgtggt aaccgcgaac
      661 gcggccaccg ggatccccat gcgccgcctg ctcgaaagtc ctgaaatacc cgcagatgtt
      721 tggccggccg aattcgttgt tgaacgcggc gccgccgatc gggccttcga tcatgatgtc
      781 gagcgccgag acgatgcgat cgggtttgcc cagtggcgca ccaggcggcg tctcccaggg
      841 ctgttcaaat cctggaatgc gcagatgcga aacggagaag ccggtgagac ccgctttcgg
      901 cttcgcgccg cgccccgtgg cgccttcatc gcgaatctct ccaccggatc cggttgcagc
      961 tcctggaaac ggtgaaatcg cagtcggatg attgtgcgtc tcgaccttca tgagaatgtc
     1021 gatgggctcg tcgatcgcgc cgtagatgtc gttttccgga ttcgggaagt atcgcttgcc
     1081 gagagcgcct tccatgacgg cggcgttgtc gcgatacgcc gagagcacgc cctgcggatg
     1141 cttctcgtgg gtgttgcgaa tcatcgcgaa cagcgatttc ggctggcgtt cgccgtcgat
     1201 gatccagtcg gcgttgaaga tcttgtggcg gcagtgctcg gagttcgcct gcgcgaacat
     1261 catgagctcg acatcggtgg gatcacggcc gagcgctgcg aagttcttca ccaggtagtc
     1321 gatttcatcg tcagacagcg cgagtccgag ctcggagttc gcggtgacca gtgcgtcgcg
     1381 tccgctcgcg agcgacacgg tgcgcaaggc gcgggttttt tccctgacga acagccgcgc
     1441 cgcgtcgccg cggtcgagca atgcagcctc ggtcatgcga tcgaacaacg gagtggcaag
     1501 ccgcatcgct tcttcgtgcg acagcgcttt cgtgctgccg atgcgccaga cgattccacg
     1561 ctcgacgcgg cgcaccgcgt cgagtccgca cacctgcgcg atgtcagtgg ccttgctcga
     1621 ccagggcgaa atggtgccga agcgtggtac gaccacgatc tcggtggtgg cggcagacga
     1681 gtccagcgcc ggcgcgtctt cgcgcggacc gtaggtcagc aggcgctcga gcagagtggt
     1741 ttcgcgcgca tcgagtttcc gcgccagatc gacgaagtgc acgaagcggg cgctgatggc
     1801 gcctattttg tgatcaatgg cgggataaat ggcttcgagc gcgggacgca gttttgcgat
     1861 gcgaaaatcg gagactgcgg aggcgccgag gagctcgatc attgagggcg cgaattgtac
     1921 agaatgtaaa aaggggacag atctatttat tggccctcgg cgctgagaat cccagccgtc
     1981 cgcggttcaa taaatagatc tgtccccttt ttacttcttg gtgcccggat cgctcgccgg
     2041 cgtgtacatc gacatcggaa atgccgagac gttgttgccc gcgaagtcgc tcaccttgct
     2101 cacaccctgc tttttgactt cgtcgatgcg caggatggag tgcatgggca gcatcgtgca
     2161 cttgacgccg gagaactcgg atttgatgcg ttcttcgccc gggtccagga ccacggtgga
     2221 tcgttcgcca aagaccagtt cttcgacttc gatgaatccg aacaaccgac cctgggccac
     2281 cttacgggca taaacctcgt aaaccttgcc ttggttcacg aacataatct tgaaaatgtg
     2341 actggatgcc atcgagccga ctaggggggc ggtaacctgc cccttcatac acgcatgggc
     2401 agcgggaagc tagggcccag agggtagcaa atggcgctca ggttgcgcgt catcagtgat
     2461 catcgccaca ggttgggcga caaaagtacg ttcgtctttg gcgtctcggg cggaagcatc
     2521 ggacgctcgg ccgagaacga ttgggtactg ccagacgaca tgagatatgt gtcgggccgg
     2581 catgcgcgca tcgtttatca caaaggtcga tacatgctgc aggacaccag cagcaacggc
     2641 acgtacgtga acgaccagga gcgcccgctc ggcaaccaga atccgcacac gctttcgagt
     2701 ggcgacgtca tccggattgg cgaataccac gtccaggtgc agatcgactc ggctaccgat
     2761 ttcagtctcg acgacagcgc gctgtacaag accggctcga ccagcacccg cagccgccgc
     2821 gtgccccagc ccaaggatct tggcgcgagc ctgcgcctcg agaacctgct cgaggccagc
     2881 aacgacatct cgagcgacga actcaaaccc gtcaacgcat tcggccaggc ggtttcggcg
     2941 cgcacccgga cgctgcatca gacgcaggac cttcccaagg agcccacgac ggcgccggag
     3001 ctcgaagtcg actcggaggc cgtggcgcga cgcattgcgc gtcttgccaa ggccgcgacc
     3061 aagcaacaac aggcattgca gcagccgacg gcaccagccc cctccgcacc cgcgccgact
     3121 tacgcgcagt ccgccgtgga caacacgccc ggattgcagg cgttctgccg cggtgccggc
     3181 attgggcccg aaaccctgcc cgtggaagca cactcgcgca tgttgcatct cgccggccag
     3241 ctgttgcgtg aatcgctgct gggactgaag gagagcaccc gcagccagca ggagcaacgc
     3301 aaccacctgc gagtcaccta tcagaaaatg cccggagacc tgctgccctc gctcgagcgt
     3361 cacagcgtgg atgaactcat ccaggaactg ctgaaggcgc acgacagccg ccgcttcgac
     3421 gccgttcagt ggttgcgtga atcgttcgcc ggcggccgcc gccacgacga ggccatgatc
     3481 aacgccatgc gcgctgcatt cgtggatttc atgggccggc tcgatccacg tgatctcgcg
     3541 acgcgtttcg agcgcagcgc gcgccgcaag accatgggca actgggaact gtacggagac
     3601 ttctatcgca gtctctgcga gatgcagccc ggcacgctgc cacacatctt tgtcgaaacc
     3661 ttcgcccaga actacgagca ggccgcccgc gacgccgaag gcggcgccac gcaacacgac
     3721 gccgcctgag cgcctgattc tgcctctcgc cgcgtccgcg gcttgtgaaa tccagcttcc
     3781 ctgtcgaaac tgcccgctgc cattcgttta gggctcaaag gagccacgac aatgaagcag
     3841 taccttatct ctgtgtgtta tcccgccggc ggcacgcagc cgccacccga cgcgttgcag
     3901 cggatcatgc gcgacgttca cgtcatccag aaggaaatgc aggccgccgg cgtgtggatc
     3961 ttcagtggtg gcctcactcc gccggattct gcaaccgtat tgcgccatca gggcggtgac
     4021 gtgatcacga cggatggtcc tttcatcgag agcaaggaac agattggcgg catcaccatt
     4081 ctgaaggcgc ccgatctcga cgcagcgctg gcgtggggcc gcaaactcgc gcgcgccatc
     4141 ggcactccga tcgaagtgcg cccgttcgcg gagggtcact gagcgcgcag atcgaaacga
     4201 ttttccggcg tgagcatggc cgtgcggtcg ccgttctggt ccgttttctc ggcgacatcg
     4261 ggctcgccga agaagcggtc caggacgcgt tcaccattgc gctcacgcac tggccacgca
     4321 gtggcatccc gccctcaccc gccggctgga tcatcaccac cgcgcgaaac cgcgcactcg
     4381 atcatcttcg ccgtgaagcg agccgcgatg aacgccatcg cgccgccctg cagctcttcg
     4441 agcaagaaac ggaagaggag cacgaggtgc gtgacgagcg gttgcgcctg attttcacct
     4501 gctgccaccc ggccatcggc accgaagcgc agcttgcgct gacgcttcga ttgttaggcg
     4561 gactcacgac ggcggagatc gcgcgcgcgt tcctggttcc cgaagccacc atggcgcagc
     4621 gcctgtcgcg cgcgaaagcc aagatccgcg atgcaggtat tccgtatcgc gtgcccgaag
     4681 gtcctgaact gcctgcacgc gtgcgcagcg tgctggccgt cgtgtatctc atgttcaacg
     4741 agggctacag cgcgacctcg ggtgaatcgc tggtccgcgc cgagttgtgt gacgaggcaa
     4801 tacgcctcgg ccggctgctg gtcgaaatgc tgccaggaga gcccgaagcg ctgggcctgc
     4861 tcgcgctgat gctgctcatc gacgcacgcc gcgaagcgcg tgtcgacgaa tccggcggcc
     4921 tcgtgcggct cgccgagcag gaccgcgacc gatggaatcg tgcggaaatc gacgaaggtc
     4981 aggcactgat gcgtgcttgc ctcgtgcgaa accagccggg cccgtaccag atccaggctg
     5041 cgatcaacgc cgttcacagc gacgcacggc gcgccagcga gactgactgg cgccagattc
     5101 tcgagctaca cgaccagctc ctggcgctcg caccgagccc cgtcgccgaa ctgaaccgcg
     5161 cggtggtggt tgcagaaatc gacggcgctc aacgggcgct cgagcgaatc gagcccttgg
     5221 cgctcgacca gtaccacctg tttcacgccg tgcgcgccga cctgttgcgg cgactcggac
     5281 gcgatgccga tgccgcggcg gcgtaccagg cggcaatcgc gctctgcgac aacacgcgtg
     5341 agaaaggatt tcttcaggcg caatacgacg cgatagccag gaactgactc agccggcgag
     5401 caattgcttc tgcggagctt cgagagacaa cagctgcgcc tttgcgtcga gcccgccgcc
     5461 gaaacccgtg agatcgccgt tcatgccgac gacgcggtga cacggagcga tgatcgaaat
     5521 cggatttcgg ccgttcgccg cgcccacggc gcgcaccgcg gcgggattgc cgatttgttc
     5581 ggcgatctgg cgatagctgc gtgtttcacc gtaaggaatt gtgagcagtg cggcccagac
     5641 ccggcgctga aaatccgtgc cgcggaagtc gagctcgaca tcgaacacgc ggcgcgagcc
     5701 tgcgaagtat tcgcgcaatt ggcgcgcggt gtcgcgcagc acgggatgcg cgggcttttc
     5761 cactgcgttt tcgaacgcgc cgcgatggcc ttcgcgtcgc cacaccagcg cgaccagcgc
     5821 gcgatcggtg gcgacgagcg ataacgcacc cacaggagag tcgatggtgc agctgtaatg
     5881 cttggagacg tcggttgagt tcatgggaaa agagtagcgc gcgaaggggc gggtgagaaa
     5941 cccgattcct gctgagaagg gggagaaaga ggggaagcgc gagagtcacc gagggtgaga
     6001 ttattcgcga cgggggaccg tcgctcaccg ctcgcttcgc tcgggccaca ctcgcgaaga
     6061 cgctcgtgtg ttcagctttg ccctgcaaag ctgtgttctc gggtggaact gtcgaccctg
     6121 tctcgattca tgcatgtgag tccgaatctc acccgcgatg acgtcactat gtagaaatgg
     6181 cggagagggt gagattcgaa ctcacgtgcc ggaattaccc gaccatccga tttcgagtcg
     6241 gcgccgttat gaccgcttcg gtacctctcc gcgccagaac cttttacaag ggcctcagac
     6301 ggcgcctcca aattagggcc gcccttcgcg gggcgcgtat tctagccgaa cgatgcacag
     6361 agccaagggg cggaaaaacc tgacgaaccg gcggaccttt ttggccctcg gaatcgcagc
     6421 cctgggcctg gggcccccgg tgctgggcgc cgaacatccg gcgccagcca agcccaaacc
     6481 caagcccgcc cctgccaaac cgcccgtgca gcgcgcacgg ctgcctaagc gcctggcagc
     6541 cgatttccgc agccatatcg agacccggct tccccatctg cgctcgtcct tcgaggccgc
     6601 gggccgggac acggggctca actggcggat gctcgcggcg ctcggttatc aggaatcgcg
     6661 ctggcggccg gcggcggtat cgccccgtgg cgcgcagggc gtgatgatgc tcatgccgct
     6721 gaccgccacc aagatgggcg tgaaaaacgt gttctccccg gacgagaaca tcatggcggg
     6781 ctcgcgttac ctgctctaca tgaaggagcg cattccaaag cgcatccgcg atcccgaccg
     6841 cacgctgctc gccatggccg cgtacaacat cggcatcggc catctcgagg acgcgcgcat
     6901 catcacgcag atgcgcaaga agaaccccga cgtgtgggct gacgtgcgcg ccaaccttcc
     6961 gcggctgtcc gacccggtgt ggcacaagcg cgtcaagcgc ggttatgcca acggccggga
     7021 gaccgcgcaa ttcgtcgaac gcgtctcgca gttcgcggcg attctcgagt cgtccgtgcc
     7081 cgacgcggtc gtcgccaggg attgatccgg agatctctag ctacttcagt tcgcggatct
     7141 tgatgctgcg gaacgaaacc gcatcaccgt gatcctgcag caggatgcga cccttctgcg
     7201 cctcgccgaa acccggcgtc gatttgaact tgccggctgc gacgtgcgcg cggtagtcgt
     7261 ccgaaccgcg cttgaattcc agcaccttca cgccgttcag ccagtgttct accgtgccgt
     7321 cggcgcgcgc cacgatgcgc gcgtgttgcc acacgtcgat cctgggagcg atgcccacat
     7381 tggtcatgag cttggcgcgc ggatagatgt cgtacagcga agcgagcgtg cgattgccgt
     7441 cgatgccgag ctttgcatcc ggatggcgct catcgtcgag aatctggtat tcgagcccga
     7501 gcggcgcgcc gctcacagga tctttcggcg aggtcagcaa gtagaagatg ccgctgttcg
     7561 tgcctgccga caccttgaat tccatctgca ggtcgaaggc gccgaattcc tcgtcggtca
     7621 tgatgtcgcc gccaccgcct ttcgcctgca cggcgagcac gccgtccgcg aatgaccagc
     7681 ccttggccgg aaatccttcc gcgttcgcgc tgcgccagcc gcgtgtggtc ttgccgtccc
     7741 acagcagtcg ccatccctgc gccttctcct cgggatcgag gttgttaggc agcaggttgc
     7801 ggatgaaaat gcccatgggg gccctgcgct tcagattctt cgtctgcacc tgcaggttct
     7861 tccaggtcac ggtgcgcccg gcttcttccg cattgttgat gctatggacc tgcaagccga
     7921 aaaatccctc cttggtcgca tcgtcgatga catccgccgc gggggcgtcg ttgatccaca
     7981 cgcgcaggcg gggtccgacg gcttcgatgc ggtagtgatt ccactcgccg aacttgtaga
     8041 gcgccttggc cgggggatgc agctcgccgg tgtagaacca gccccgctgc gcctcttcat
     8101 aaatgctgcc gctccactgc cgcggcgaag gatcgatgtc ggtctggtag ccatggacgc
     8161 ggccattttc gaagtccggc gtgctcaggc tgcggaactg caccccagaa ttggtggggc
     8221 cgacgtcctg acgaacgtcg aattcgaata tgaagtcccg gtagaccttc tcggtgacga
     8281 gaaagctgtt gggaattcca gggcgcgaag atccgacgat ggcgccgtcg atcaccttgt
     8341 aatcggcgcc gccaccgaga gtcttccagc cggtcagcga cttgccatcg aagatgggcg
     8401 tggcgtcatt cttgtccgcg ctggcggcag tcacaccgaa cgccagcatc gccagcgcgc
     8461 aaaaaaactt ccgcattcct ttccccttcg tcagcaccag cgaatagttg gtttcaatcg
     8521 gccttgaacg cttcgacctg gatggcaagt ttcacttcca tcttgaaacc gaacgccttg
     8581 ccccagttga tgccgaagtc ttcgcggctg aaattgccca ccgcatcggc gccacagaat
     8641 tccttcttct tcattgggtg cgccatgcac ttgaacgaac ggatggtgag tgtcacgggc
     8701 ttggtgacgc catgcaaagt gaggtcgccc tgcacttccg tgggcgcgcc atccttgaac
     8761 ttggcgagct ttcccttgta ggtggcggtg ggatacttcg cgacatcgag catgcccgga
     8821 tcggtgccct tcagatgcgt atggagcttc tcgagcccga ggtcgacgga gtccatgtcg
     8881 atggtgatgt cgaccgtgcc gctctgcgct tcgcggtcga gcactatggt gcccttgttc
     8941 tcattgaatt ttccgcgcca caccgacaca ccaccgaaat gatctacctc gaaactcgga
     9001 taggtgtgtt cggggtccac gttgtaggtg acgggagcgg caacggcgcc agctgctgca
     9061 gcgccggcaa tcatgcaaag gagcaggcga attggggaac gcatggagaa tatcctttat
     9121 gaggccgcgg gcgcgggacc gcgacgctag cacaccgatc tctcgatctc ctcctcctga
     9181 gtgacctgag tgaagaggac gcccctcccg tcgctatgtc agctcccgcc gacgcgcagt
     9241 gaagaaagca ttcagcattc gcgcgctttc ctccatgaga acgccgccaa aaacatccac
     9301 gcgatggttc atggacggca tggtgaatac gttggtggta ctgcccgcgg cacccgcttt
     9361 gggatcccag gcgccgaata ccagccgcgc cacgcgggca tggacaatgg ccgacgcgca
     9421 catgacacag ggctccaggg tcacgtacag cgtggtgccg ttgagccggt aggatcccag
     9481 cgtcttgccg ccggcgcgca atgcttcgat ctcggcgtgg gcggtcggat cgtggccggc
     9541 gatcggccgg ttcgccgcca ccgcaagaat ggcgtctccc tgtacgagca cggcacccac
     9601 gggcacctcg cctgcctgtt cagcgccgcg cgcggcctcc agggcgaggc gcatgaaatc
     9661 gatgtcgttg cgtagtggca tgtcagcttg ggggaagtcc ttagtgactt aaccctatca
     9721 aactactagt gttttgtgtc gctcgaccag aaggttcgga acatgaacac cgctgaaagc
     9781 aatgatccgg cgaccgacat tctcagtcag cacgtcggcc cgcggcgcga gggtgacgcg
     9841 agcatcattg cgaacgagat cgacatcgcc atgtcgccga tctccctcta tgcggacgcg
     9901 ctgctcgaac gcaaagatct cgacaaagct gcgcgccagt acgtcacgag cattcgccgc
     9961 gcggccgatg acgtggcgct cgcggttgcg cgattgcgcg cgctcgatcg cccgcatacg
    10021 ccggcgccgg aagccccgga aagccgaaaa caaacgccgg cgcctgccgt tcgaagcctt
    10081 cgcgtgttgc tcatcgatga tgatccttcg ctgatcgaat cattgcgcag cgccctggtc
    10141 gatgaaggcc acaaagtcac gaccgccaat ggtggccagg cgggaatcga cgcgttccgc
    10201 gcagcgtccg cgtcccgcat gccgtacgac atcgtgatca cggaccttgc catgcccgac
    10261 gtcgatggcc gccaggtcgt cgccagcctg cgcgccgctt caccaggcac tccaatcgtg
    10321 ctgctcaccg gctggcgtca tcagctggcc gatcgcgtcg agcgcccgtt acaagtggac
    10381 cggctgctcg gcaaaccgcc gcgcattcgc gaactgcgcg cggcgctggc cgaactcacg
    10441 gggcgtcgcg cgaccgatcg actcgactga tactgaccgg cgcgccatca ggccggcatt
    10501 gcaagcgcaa tcacaccggc gacgatcagc gcagcaccgg caatgcgcca gggccctgag
    10561 tcctcgcgca acaggcgcga gccgaaccat gcgcccacca gcgttgacaa ctcccgcgcc
    10621 ggagcaacgt gactgatggg cgccatgcgc atggcgaaca acaccaggat atagccgagc
    10681 ggcccgagca cactcacgat gatgatgggc ttgcgataga ggcgcgcttc gagaccgacg
    10741 cgcgcgcgat cggcccaggc gcgcggcgcg agcaccacca tgcgaaacag attgccggtg
    10801 aagtcgatga gaaacggtga gatcagcagc accttcaccg cgtaaccatc attcacggtg
    10861 tatgcagcga tgaagacgcc ggtgccgagt ccgtagagga tgccggcgcg cggcgccttg
    10921 tgcggctcgc tcgtgagacc ggcaacgagc aggatgcccg cgacgatgag cacgagcccc
    10981 aggatcgaca acgacgtcgg ctgctcgccg aggaagatcg ccgcgcccac gaacgacaac
    11041 agcgggcccg ttccgcgcgc aatcggatac acgagggaga gatccgaggc gcggtaaccg
    11101 gcctgcagga ccagggagta gcccagatgg agcacgctcg tggcgccgag cgccagccag
    11161 tgttgtgccg tgaattgcgg ccgctcggtg aaaagcagcc acgcgacgat gggcgcgtag
    11221 aggatcacgg aaccgagcga atacagccac acgaaatgac gcgaggcgac tgcggacttc
    11281 gcgaagaagt tccaggtcgc gtgggtcagc gccgcgagca gaacgaagct cagcgcaag
//
LOCUS       DASQJS010000310         3177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3958541, whole genome shotgun sequence.
ACCESSION   DASQJS010000310 DASQJS010000000
VERSION     DASQJS010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3958541"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /gene="araD1"
                     /locus_tag="VEW08_12520"
     CDS             <1..778
                     /gene="araD1"
                     /locus_tag="VEW08_12520"
                     /inference="COORDINATES: protein motif:HMM:NF040903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraD1 family protein"
                     /protein_id="HYJ41609.1"
                     /translation="PLTHPDPSHCLVSGTGLTHLGSAAARDAMHAKLTKTDDLTDSMK
                     MFKAGVEGGKPKSSKPGIQPEWFYKGNGRNVVGAGSPLKSPDFAEDHGEEPELVGVYV
                     IDDNRRPHRLGFAIGNEFSDHVTERRNYLLLAHSKLRQCGVGPMLNTGTLPSHLEGTS
                     RIRRDGAVLWQKSFLTGEANMCHTFANLEYHHFKYKQHRVPGDVHLHFFGTATVSFAD
                     SVVAQAGDEFEIDLPALGAPLINKLLIEPGGLVLHDTGTL"
     gene            793..1746
                     /locus_tag="VEW08_12525"
     CDS             793..1746
                     /locus_tag="VEW08_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ41610.1"
                     /translation="MKFSRRLAIIAALLIAAGCSRESKDERIVLGFSQIGAESEWRTA
                     NTESIKAAAATSNIDLRFADAQQKQENQIKALRSFIAQKVDVIAFSPVVATGWDTVLM
                     EARDAGIPVILTDRAVTSDPSLYVGFLGSDFVEEGRKAGRWVAEQFATVAQDVNIVEL
                     QGTVGSAPANDRKKGFEEIIAANPRLKIIRSQTGDFTRTKGKEAMEAILKSESRKIHV
                     LYAHNDDMAIGAIQAIEEAGLKPGQDILIVSIDAVKGAFQAMIAGKLNATIECNPLLG
                     PQLMTSVTEVVSGRPIAKRMVVEETMFTMETAKQHIQSRKY"
     gene            1757..>3177
                     /locus_tag="VEW08_12530"
     CDS             1757..>3177
                     /locus_tag="VEW08_12530"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HYJ41611.1"
                     /translation="MNDPAIVLRARDISRAFPGVQALSGAELTLRAGEVHALMGQNGA
                     GKSTLIKILTGVYAPDAGSIELLGNAVTPRSPIHAQELGISAVHQESHLLPNLSVAEN
                     ICAGKYPRRPWTRGGGIDWRETGRRAAELLASLGINLDVAQLAGPLPAALQQLATVAR
                     AVSTDARVLILDEPTSSLDADEVQALFALIRRLRDKGVAILFVTHFLDQVYEICDRMT
                     ILRNGKFVGEYACAKLDTHGLVAAMVGRELAATTRSTATEPADGNAATPPVLAARNLS
                     RRGQLKPMDVELKRGEVLGLAGLLGSGRTELARLLFALDKRDTGDISIDGKPAKLDHP
                     ADALQLGLGFCPEDRKHSGIIADLSVRENIALALQARMGLTKFLTVAEQRQIAGKLVA
                     ALGIKTSSIETPIGQLSGGNQQKAIIARWLATHPRVLILDEPTRGIDIAAKQELMNEI
                     LDLARGGTAVLFISAEIDEVVRVS"
BASE COUNT          642 a         1069 c          978 g          488 t
ORIGIN      
        1 accgctgacg catccggatc ccagccattg cctcgtgagc ggcacgggcc tcacgcatct
       61 aggcagtgcg gcggcgcgcg acgcgatgca cgccaagctc acgaaaactg acgacctcac
      121 cgactccatg aagatgttca aggccggtgt cgaaggcggc aagccgaaga gcagcaaacc
      181 cggcatacag ccggagtggt tctacaaggg caacggccgc aatgtcgtgg gcgcaggttc
      241 accgctcaag agccccgact tcgccgaaga tcacggcgaa gagcccgaac tcgtgggcgt
      301 gtacgtcatc gatgacaacc gccgtccgca ccggctgggg ttcgccatcg gcaacgagtt
      361 ctccgatcac gtcacagagc ggcgcaacta tctactgctg gcgcactcca agctgcgcca
      421 gtgcggcgtg ggtcccatgc tcaacaccgg cacgctgccg tcgcacctgg aaggcaccag
      481 ccgcattcgc cgcgatggcg cggtgctgtg gcagaagtcg ttcctcaccg gcgaggcgaa
      541 catgtgccat accttcgcca atctcgaata ccaccacttc aaatacaagc agcatcgcgt
      601 gcccggcgac gtgcacctgc acttcttcgg caccgccacc gtgagcttcg ccgacagcgt
      661 cgtggctcag gcgggcgatg aattcgaaat cgaccttccg gcgctgggcg caccgctgat
      721 aaacaagctg ctgatcgaac cgggcgggct cgtgctgcac gacaccggga cgctctgacg
      781 cagggagcaa cgatgaagtt ttcacgacgt ctcgcgatca tcgcggcctt gctcatcgcg
      841 gcaggctgca gccgggagag caaagacgag cgcatcgtgc tcggattcag ccagatcggc
      901 gccgagagcg agtggcgcac cgcgaacacc gagtccatca aggccgcggc cgccaccagc
      961 aacatcgacc tgcgtttcgc cgatgcccag cagaagcagg aaaaccagat caaggcgtta
     1021 cgcagcttca tcgcccagaa ggtcgacgtg atcgcgttct cgcccgtggt ggccacgggc
     1081 tgggacacgg tgctcatgga agcgcgcgac gccggcatcc cggtgatcct caccgaccgc
     1141 gccgtgacca gcgatccttc gctctacgtc ggctttctcg gttccgactt cgtcgaagaa
     1201 ggccgcaagg ccggccgctg ggtcgcagaa caattcgcta ctgtcgcgca agacgtgaat
     1261 atcgtcgaac tgcagggcac cgtgggttct gcgcccgcga acgatcgcaa gaaggggttt
     1321 gaagaaatca tcgccgccaa tccgcgcctg aagatcattc gctcgcagac cggagacttc
     1381 acgcgcacca agggcaagga ggccatggaa gccatcctca aatccgagtc gcgcaagatc
     1441 cacgtgctct acgcgcacaa cgacgacatg gccatcggcg cgattcaggc catcgaggaa
     1501 gcgggtctca aacccggcca ggacattctc atcgtgtcca tcgacgcggt gaaaggcgca
     1561 ttccaggcca tgatcgccgg caagctcaat gccaccatcg agtgcaatcc gctgctcggc
     1621 ccgcagctga tgacgtctgt gaccgaagtg gtgtcgggac gcccgatcgc caagcgcatg
     1681 gtcgtcgagg aaaccatgtt caccatggaa accgcgaagc aacacattca gtcgcgcaag
     1741 tactgaaccc catgcgatga atgacccagc gatcgtcctt cgggcgcgcg acatctcccg
     1801 cgcctttccc ggcgtgcagg cgctgtccgg cgcggaactc acgttgcgcg cgggagaagt
     1861 tcacgcgctc atgggacaga acggcgccgg caagtccacg ctgatcaaga tcctgaccgg
     1921 cgtctacgcg cccgacgccg gcagcatcga actgctcggc aatgccgtga cacccaggtc
     1981 gccgattcat gcgcaggagc tcggcatcag tgccgtgcat caggaaagcc acctgctgcc
     2041 gaatctctcc gtggcggaaa acatctgcgc cggcaagtac ccgcgccgtc cctggacgcg
     2101 cggcggcggc atcgactggc gcgagaccgg gcggcgcgcg gcggagttgt tagccagcct
     2161 cggcatcaac ctggacgtgg cgcagctggc ggggccgctg ccagccgccc tgcaacagct
     2221 ggcgacggtg gcgcgcgcgg tgtccaccga cgcacgcgtg ctgatcctcg acgagcccac
     2281 gtcgagcctc gatgccgatg aggttcaagc gctgttcgcg ctcatccgca ggctgcgcga
     2341 caagggcgtc gcgattctct tcgtcacgca ttttctcgac caggtgtacg aaatctgcga
     2401 tcgcatgacg atcctgcgca acggcaagtt cgtcggtgag tacgcctgcg cgaaactcga
     2461 cacgcacggg ctggtcgcgg ccatggtggg ccgcgagctg gcggcaacga cgagaagcac
     2521 ggccaccgaa cccgccgacg gaaacgccgc cacaccgccc gtcctcgccg cgcgcaacct
     2581 ctcccgccgc gggcaactca aacccatgga cgtcgagctc aaacgcggcg aagtcctggg
     2641 actcgccggc ttgctaggct ccggccgcac cgagctcgcc cggctgttgt ttgcgctcga
     2701 caagcgtgat accggcgata tcagcatcga tggcaagcct gcgaaactcg atcatcccgc
     2761 ggacgcgcta caactcggac tggggttctg cccggaggac cgcaagcaca gcggcatcat
     2821 cgccgacctg tcagtgcgcg agaacatcgc gctggcgctg caggcccgca tgggcctcac
     2881 gaagttcctc acagtcgccg aacagcgcca gatcgccggg aagctggtcg cggcgctggg
     2941 gataaaaaca tcgagcatcg aaaccccgat cgggcaactc tccggcggca accagcagaa
     3001 ggccatcatt gcgcgctggc tcgcgacgca tccgcgcgtg ctgatactcg atgaacccac
     3061 gcgcggcatc gacatcgccg ccaagcagga attgatgaac gagatcctgg atctcgcgcg
     3121 cggcggcacg gccgtgttgt tcatttccgc ggaaatcgac gaagtggtgc gcgtgtc
//
LOCUS       DASQJS010000311         4992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3335400, whole genome shotgun sequence.
ACCESSION   DASQJS010000311 DASQJS010000000
VERSION     DASQJS010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3335400"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="VEW08_12535"
     CDS             complement(<1..1336)
                     /locus_tag="VEW08_12535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HYJ41612.1"
                     /translation="MQTTLGQGHGPVTFENSMEPYAQLVRSLLPRAASVSLFDNTGKL
                     LWSSAASANPDLFALVLQAIAHIEDNCDGVGQHCALADDGPAYLFWLRDAGSNVAAIV
                     AVVCNRLKVDADVLPFSYVHDHLKPALELLRRDLLARADIDMLNESLTARDKDLELLL
                     SVTGSHPEASGADDLQSLLANAAGHLKCVLAGIVVPDKGLVLTHPETGDATILTRTQK
                     QLLAITQTRRDPLVINRIATQAGQVAIPYRILACPLRQGSGRTSGVLALFRKLEAPEF
                     IGRDVHLADLLSRRVAASIETSYDTLSGLLTRAALEQRVKLMLTEGDPRGQRWSLLYV
                     DIDELHVINENFGMHVGDQAIAQIGDLLRKHLPASSFASRISGDRFAVVMPSEIDEAA
                     MFGEGLREGVTRLHTVGVDARFRLSISIGVTPYSGRDSDLGRSLAEAESACKA"
     gene            complement(1372..2583)
                     /locus_tag="VEW08_12540"
     CDS             complement(1372..2583)
                     /locus_tag="VEW08_12540"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYJ41613.1"
                     /translation="MTISSSRPGRAQFGRTRFGRAVFAPLFLALCLASTAPAADEANP
                     AKAEAELKAVRSQIEKMQAELERDAGRRDKITRELEESEKTVSGARGELDKLRRERAV
                     HTARRAELAEARRKEEAELAERRAALAGQIRAAHMIGQEEPLKLLLNQRDPAQTGRVL
                     TYYQYFGRARAGQIAAINSHIAELATLDAGLAEEEARLAALEEAQKGELSRLQGARER
                     RGRVLVSLETESKNRARELARLKDQQGGLEKLVRELRRALERIDKFPSDSKDAFAKLR
                     GKLAWPVAGKLLASFGQNRAGGVKWDGVLVSGSQGAAVRAVYHGRVVYADWLSGLGLL
                     TIIDHGDGYLSLYGHNERLYKEVGERVTAGDTIATVGDSGGRSTPALYFEIRKAGRPV
                     DPRPWFKSASP"
     gene            2686..3111
                     /locus_tag="VEW08_12545"
     CDS             2686..3111
                     /locus_tag="VEW08_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYJ41614.1"
                     /translation="MERLIEYVTHHPLMAASAVLALVVLVIYESRVRASGFAAISSQD
                     LIRLMNQGALVLDIRKAEEFAAGHVNGAKQLPSDQILTAGDNFKRFKEKPVVVYDHAG
                     SLAAAAVRQLNQQGFTKAFSLRGGFAAWRAEQLPVTKAS"
     gene            3111..3371
                     /gene="grxC"
                     /locus_tag="VEW08_12550"
     CDS             3111..3371
                     /gene="grxC"
                     /locus_tag="VEW08_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HYJ41615.1"
                     /translation="MSSPAVVMYTTSWCPYCERARKLLATKNVTISEIDVESAPEKRA
                     EMRIRSGRTTVPQIFIGDHHVGGSDDLLSLEDTGKLDGLLGR"
     gene            3406..3891
                     /gene="secB"
                     /locus_tag="VEW08_12555"
     CDS             3406..3891
                     /gene="secB"
                     /locus_tag="VEW08_12555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HYJ41616.1"
                     /translation="MENQTAPAANENPTGPMLAIQSVYLKDCSYEAPKGPRVEGNWSP
                     QISLDINTTASVIAPELSEVVLTVTVGAKQGEVTAFLVEVKQAGLFAMRGLTADDQRR
                     AIGTICPNVLFPYARVAVSQLVTQGGFPNFVLPPVDFDQLFAKGLQQQAAQAGTQGPA
                     N"
     gene            3909..4964
                     /locus_tag="VEW08_12560"
     CDS             3909..4964
                     /locus_tag="VEW08_12560"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HYJ41617.1"
                     /translation="MATRLLSSTHTHLAGNDIAHESVAVLGAGSWGTALAIQFARAGH
                     PTRLWCRNAADAAQMTADRHNRRYLPDARFPDAIEVHTDLGAAVRNAVAIIVAVPSHS
                     LRALLTQLKPLLARDARLAWATKGFELDTGKLPHQVAYEVFGDRYPVAVLSGPTFARE
                     VGMGLPTAMTIASPDADFASQLAKSLHASNFRAYTSTDIVGVEVGGAVKNVLAVGAGL
                     SDGLGFGANTRIALITRGLSEVMRLGVALGAQPETFMGLAGLGDLVLTSTDNQSRNRR
                     FGLALAGGKTVEEALKEIGQVVEGYSAARAVHEVARRENVDMPIVSGIYRVLYGNEPA
                     RDVVKDLMTRPIRPEVE"
BASE COUNT          822 a         1659 c         1650 g          861 t
ORIGIN      
        1 ccgccttgca ggcggactcg gcttcggcga gcgaacggcc aaggtcgctg tcacggccgc
       61 tgtagggagt gacgccaatg ctgatagata gacggaagcg cgcgtcgacg ccgacggtat
      121 gcaggcgcgt gacgccttcg cgcagtcctt cgccgaacat cgcggcttcg tcgatctccg
      181 agggcatgac gacggcgaag cggtcgccgg agatgcgcga ggcgaacgag ctcgccggca
      241 ggtgcttgcg cagcaggtcg ccgatctgcg cgatggcctg gtcgccgacg tgcatgccga
      301 agttttcgtt tatgacgtgc agctcgtcga tgtcgacgta cagcaggctc cagcgctgtc
      361 cgcgcggatc gccctcggtg agcatgagct tcacgcgctg ttcgagcgcg gcgcgcgtca
      421 gcaggccgga cagcgtgtcg tagctcgttt cgatgctcgc cgccacgcgc cgcgacagca
      481 ggtcggcgag gtgcacgtcg cggccgatga attccggcgc ttcgagcttg cggaacaacg
      541 cgagcacacc ggaagtgcgg ccgctgccct ggcgcaacgg gcaggcgagg atgcgatagg
      601 gaatggccac ctggccggcc tgcgtcgcga tccggttgat gaccagtgga tcgcggcgcg
      661 tctgcgtgat ggctaacaac tgtttctgcg tgcgcgtgag gatggtcgcg tcgccggttt
      721 ccggatgcgt gagcaccagg cccttgtcgg gcacgacgat gccggccagc acgcacttca
      781 ggtggccggc cgcgttggcc agcaggctct gcaggtcgtc ggcgccactc gcctccggat
      841 gactgccggt gaccgacagc agcagctcga ggtccttgtc gcgcgcggtc agcgactcgt
      901 tgagcatgtc gatgtcggcg cgcgccagca gatcacggcg caacagttcg agcgcgggct
      961 tcaggtggtc gtgaacatag gagaagggca acacatcggc atcgaccttg agccggttgc
     1021 agaccacggc gacgatcgcg gcgacgttgc tgcccgcgtc ccgcagccag aatagatagg
     1081 cggggccatc gtccgcgagc gcgcaatgct ggccaacacc gtcgcaattg tcttcgatgt
     1141 gcgcgatggc ctgcagcacc agcgcgaaga ggtcggggtt ggcgctggcc gcgctgctcc
     1201 acagcagctt gccggtgttg tcgaacaggc tcaccgaagc ggcccgcggc aacagcgagc
     1261 gcaccagttg cgcgtacggt tccatggagt tttcgaaggt cacaggcccg tgaccttggc
     1321 ccaatgtcgt ctgcatgacg ttgatgcagt tggcgttttt tccgtcgacc cttagggcga
     1381 cgcgcttttg aaccagggcc gcgggtcgac cggccggccc gccttgcgga tttcgaagta
     1441 cagcgcaggg gtcgaccgcc cgccggagtc tcctaccgtc gcgatggtgt ccccggcggt
     1501 cacccgttca cccacttcct tatagagccg ctcgttgtgg ccgtagaggc tcaggtaccc
     1561 atcgccgtgg tcgatgatgg tcaggagacc gaggccagac agccagtcgg cgtatacgac
     1621 gcgaccgtga tagaccgccc gaaccgcagc gccctgggac cctgatacga gcacaccgtc
     1681 ccatttgacg ccgcccgcgc ggttctgccc gaatgaggcg agcagctttc cggccacggg
     1741 ccaggccagc ttgccgcgca gcttcgcgaa ggcgtccttg ctgtcgctgg ggaatttgtc
     1801 gatgcgctcg agcgcacgcc gcagctcgcg caccagcttc tccagaccgc cctgctgatc
     1861 ctttagcctt gccagctcgc gggcacggtt cttcgactcc gtttccagcg acaccaacac
     1921 gcggccacgg cgttcgcgcg cgccctgcag ccgcgacaac tcgcccttct gcgcctcttc
     1981 gagcgcggcc aggcgtgctt cttcttcggc gagtcccgcg tcgagcgtgg ccagttcggc
     2041 aatgtgcgaa ttgatggcgg cgatctggcc ggcgcgcgca cggccgaaat actggtagta
     2101 cgtgagaacg cggcccgtct gcgccggatc ccgctggttg agcagtagtt tgagcggctc
     2161 ttcctggccg atcatgtgcg cggcgcggat ctggccggcg agcgccgcgc ggcgttcggc
     2221 aagctcggct tcttccttgc ggcgcgcctc ggcgagctcg gcgcggcgcg cggtgtgcac
     2281 ggcgcgctcg cgccggagtt tgtccagctc gccacgtgcg ccgctcaccg tcttttcgct
     2341 ttcttcgagc tcgcgggtga tcttgtcgcg acggccggcg tctcgctcga gttccgcctg
     2401 catcttttcg atctgcgaac gcaccgcttt cagctcggcc tcggccttgg ccggattcgc
     2461 ctcatcggcc gctggcgcgg tggaggccag gcacagcgcc aggaatagcg gcgcgaagac
     2521 tgcccgcccg aaccgggtcc gtccgaactg ggcccggccg ggccggcttg aggaaatcgt
     2581 catgggcata ttctagagag gcagcctcgc gaaaatcggc gaaaacagcg agtgccgcta
     2641 taatgcgctc cctttttcgc gccctcccgg gggccacctg gactcatgga acgtctgatc
     2701 gaatacgtga ctcatcaccc tctcatggcg gcgtccgcgg tattggcgct ggtggtgctg
     2761 gtcatctacg aatcacgcgt tcgcgcctcc gggttcgcgg caatctcgtc tcaggacctg
     2821 atccggctca tgaaccaggg cgccctggtg ctcgatatcc gcaaggccga agagttcgca
     2881 gccggacacg tgaatggcgc caaacagctg ccctccgacc agatcctgac tgccggcgac
     2941 aacttcaagc gcttcaagga aaagccggtc gtcgtctacg accatgccgg ctcactggcg
     3001 gccgcggcgg tgcgtcaact caaccagcag ggattcacca aggcgttctc gctgcgtggt
     3061 ggctttgccg cctggcgcgc ggaacagctg ccggtcacca aggcgtcctg atgagctctc
     3121 ctgcggttgt catgtacacc acgagctggt gtccttactg cgagcgtgcg cgcaagctgt
     3181 tggcaaccaa gaacgtgacc atcagcgaga tcgacgtcga atcggcaccc gagaagcgtg
     3241 ccgagatgcg catccgcagc ggccggacca cggtgccgca gattttcatc ggcgaccacc
     3301 acgtgggcgg cagcgacgat ctgctgtccc tcgaggacac gggcaaactc gatggcttgt
     3361 taggccgctg actcttccga acagattttc aataaggaac tgatcatgga aaaccagacc
     3421 gcaccagcgg cgaatgaaaa cccgaccggc ccgatgctcg cgatccagtc cgtgtacctc
     3481 aaggactgct catacgaggc gccgaagggc ccgcgtgtcg agggcaactg gagcccgcag
     3541 atctcgctcg acatcaacac gacggcgagc gtcatcgcac ccgagctcag cgaggtcgtg
     3601 ctgaccgtga cggtcggcgc caagcagggt gaggtcaccg cgttcctggt cgaagtgaaa
     3661 caggccggcc tgttcgcaat gcgcggcctg actgccgacg accagcgccg cgccatcggc
     3721 accatctgcc cgaacgtgct gttcccgtac gcgcgcgtgg ccgtgtcgca gctcgtgacg
     3781 cagggcggct tcccgaactt cgtgctgccg ccggtggatt tcgaccagct gttcgcaaag
     3841 ggactgcagc agcaggccgc gcaggctggc acgcaaggcc cggccaactg acgctcacgg
     3901 cgggaggcgt ggcaacacgc ctcctgtcgt cgacccacac ccacttggcc ggcaacgaca
     3961 tcgcccacga atccgtcgct gtcctcggcg ccggttcctg gggcacggcg ctcgccatcc
     4021 agttcgcgcg cgccggtcat ccgacgcggc tgtggtgccg gaacgcggcc gatgccgcgc
     4081 agatgacggc cgatcggcac aaccgccgct atctgcccga tgcgcggttt cccgacgcca
     4141 tcgaagtcca cacagatctc ggggccgcgg tcaggaacgc cgtcgcaatc attgttgccg
     4201 tgcccagcca ttcgctgcgc gcgctgctca cgcaactcaa gccgctgctg gcacgcgacg
     4261 cgcgcctggc gtgggccacg aagggcttcg agctcgacac cggcaaacta ccgcaccagg
     4321 tggcgtacga agtgttcggc gaccggtacc cggtggccgt gctttcgggg ccgacgttcg
     4381 cgcgcgaggt aggcatgggt ctgccgaccg ccatgaccat cgcctcgccc gatgccgact
     4441 tcgccagcca gctcgccaag agcctgcatg cttcgaattt tcgcgcctac acctccaccg
     4501 acatcgtcgg cgtcgaagtc ggcggcgcgg tgaagaacgt gctcgcggtg ggcgccgggc
     4561 tttccgatgg cctggggttc ggagcgaaca cacgaatcgc gctgatcacg cgcggtctct
     4621 cggaagtgat gcgcctcggt gtggcactcg gtgcacagcc ggaaacattc atgggactcg
     4681 ccggcctcgg cgacctggtg ctgaccagca ccgacaatca atcgcgcaat cggcgtttcg
     4741 ggctcgcgct ggcgggcggc aagaccgtcg aagaggcgtt gaaagagatc gggcaggtgg
     4801 tcgaaggtta ttccgccgcg cgcgcggtgc acgaggtcgc caggcgcgag aacgtcgaca
     4861 tgcctatcgt ctccggcatc taccgcgtcc tttacggcaa cgagcctgcg agggacgtgg
     4921 tgaaggacct catgacgcgc cccatccgcc ccgaagtgga gtgagcggcg gcggttggcg
     4981 aactagctac cg
//
LOCUS       DASQJS010000312        13600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4269220, whole genome shotgun sequence.
ACCESSION   DASQJS010000312 DASQJS010000000
VERSION     DASQJS010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13600
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4269220"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VEW08_12565"
     CDS             <1..664
                     /locus_tag="VEW08_12565"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ41618.1"
                     /translation="ELLCERWTLLVISRVIDGCTQFNAIHRGVPRISPAMLTKRLRQL
                     EHAGLLTSRSVGKGKPRTYALTPAGQELSPLIDSLAVWGQHWARDMCMDDLDPGFLAW
                     SMHMRLDVARMPPGRTVLAFEFTGAPRDCRRFWLVNEDGCVDMCLKDPGYEVNVHVRS
                     DLRRFIETWRGIRDLRAEIRARRIELIGAPALVRKFPDWLKLSGLAVFPRQRPGRESR
                     LG"
     gene            complement(661..1809)
                     /locus_tag="VEW08_12570"
     CDS             complement(661..1809)
                     /locus_tag="VEW08_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41619.1"
                     /translation="MVWRWSRFNLVCWILLPTLALAQEPVITNEPGTADTTPPDPTII
                     SVDVTNLDPLSDDPGAGIEPGSKLDPAPEFAYEIPDPVVVPFIDRAHLGVWNAVWRSA
                     MRVDGWFGEQRGDETLYQQANGSISPALLWDKFDGLQPRLRFDVDVPLPHMNTRLHAF
                     VGRVNREEYVTERTPASGAFAQQYGPAEEDETLFGIRYRSPKQGGNFQADAGIRFRSP
                     LDPFVKGSYRFLRGSSEETLYTFRETAFWQNSEGFGFTSRMDVERVVKAWLVRWTGSG
                     TISQETEGVRGYTSLTVMRGMPNRRAIAAQIFTTGEFEAPVPTENYGVKLGYRQAVAR
                     DWLVLETRISCTFPREELTEKRITNWGVGIGLEMYFGTSEFQARPVMF"
     gene            1985..2725
                     /locus_tag="VEW08_12575"
     CDS             1985..2725
                     /locus_tag="VEW08_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ41620.1"
                     /translation="MTNKVTRSAIVTGASRGIGAAIATRLAADGFAVVVNFAGSAGEA
                     EKVVGQIEAAGGKAVAIQADVSSGDAMRRLFDETEAAFGGVDVLVNNAGIMQLAKFAE
                     ADDALFERQVAVNLKGVFNGMREAAKRLRSGGRIISFSSSVVGLYQPTYGVYAATKAA
                     VEAMTHVLANEMRGRNITVNAVAPGPTATDLFLHGKPQELVDRISKAAPLERLGQPDD
                     IAAAVAFLAGPDGAWINGQVLRANGGLI"
     gene            complement(2722..3369)
                     /locus_tag="VEW08_12580"
     CDS             complement(2722..3369)
                     /locus_tag="VEW08_12580"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYJ41621.1"
                     /translation="MRRLATFAALTLAAYAPLAAALDQNAAAPVPKGAYQIDQAHTSL
                     LFRVSHLGFSTYTGRFTRIDAKLDFDPTRLAASRVNVTIDPRSIQADNAPSGFLDSLA
                     GKDWLDAEKFPAMAFRSTNVEVTGKNSFRLHGDLTLHGVTKAIVLDARFNGGYASHPY
                     EPRARVGFSATGQFKRSDFGVSHGIPAPGTTMGVSDEVEVILESEFTGPPLVKTN"
     gene            complement(3490..4071)
                     /locus_tag="VEW08_12585"
     CDS             complement(3490..4071)
                     /locus_tag="VEW08_12585"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYJ41622.1"
                     /translation="MNQHQHPTAELLAYWLDELDEAAAAKIEEHLFECASCSAALQEL
                     VRIGDALRRELRSGNVGTVVSAEFVRRLQADGLRIREYTLRPGESVNCTVAPDDDLVI
                     SYLHAPLGEVRQVDLVFADFDGGSPHRSAHIPFDAASGVVTVVPPMAVLRAMGHARKR
                     MQLLAVEDASERVLAEYTFNHAPYDALGSGTDF"
     gene            complement(4068..4676)
                     /locus_tag="VEW08_12590"
     CDS             complement(4068..4676)
                     /locus_tag="VEW08_12590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ41623.1"
                     /translation="MNRVLSIVAREIYASPVQYVAETQDDDASLARRIAASDTGAEAE
                     FCRRFAPRIRLFGRKWLRSEAAAADLVQDVLLMALQKLRAGAVRDPERIASFMLGTAR
                     QMMVDSRRSSGRRERILDTFPVDLIPDQASATEPLDEQRLRHCLQALPERERAVLVMT
                     FYDDCPADALGRQLGLNAGHVRVIRHRGLDHLRSCMEAEGSQ"
     gene            4742..5476
                     /locus_tag="VEW08_12595"
     CDS             4742..5476
                     /locus_tag="VEW08_12595"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41624.1"
                     /translation="MITNTQSGTNIQEVADGIYRINTPVAIPGAGDFSFNQYLLVDDA
                     PLLFHTGQRQLFPLVSEAIRAVMPLDKLRYLGLSHFEADECGALNSFLAVAPQAVPVC
                     SQVAAMVAINDFADRAPQALADGAELSLGRHTVRWFDTPHMPHSWECGLMMEMTTRTF
                     FCGDLFTQGGKGEAALTESDILGPSEAFRGPMDYYAHAPQTKATLERLAREQPTTLAV
                     MHGSSWRGDGAKLLRELSVKLAATRH"
     gene            5615..7108
                     /gene="cysK"
                     /locus_tag="VEW08_12600"
     CDS             5615..7108
                     /gene="cysK"
                     /locus_tag="VEW08_12600"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HYJ41625.1"
                     /translation="MARYQNILETIGNTPVVRLNKLAPPGVNVYVKVESFNPMGSVKD
                     RMARAIIEDAERSGALLPGQTVIEATSGNTGIGLAMVCAQKGYPLVVTMAESFSVERR
                     KLLRFLGAKVVLTPAAEKGSGMLAKAVELAMTHGWFLCRQFENPANADVHSRTTAREI
                     INDFSDQRLDYFVTGFGTGGTLKGVARELKRARPGTRIIAAEPDNSQVLGSRIPQIRD
                     TAGGPAGSHPRFRPHLMQGWSPDFISTLTEQAVEAGLIDEIVPVAGAEALRLSRELAR
                     QEGIFVGTSSGATLAAALDVARRAPAGSTIVCMLPDTGERYLSTPLFESIGEEMTAEE
                     WELSRSTPLCRFDAPSAPAPAPAAAEQKVAVDPAAARFVTEVLEKEPVVMFALEWCEF
                     CWSVRKLFTKLGIAYRSVDVDSVEFQKDDLGGRIRGVLADRTGAKTLPQVFVGGEHIG
                     GATDTFDAWRNGTLQGRLDKKGVSYDREAKLDPYSLLPKWLQPRKQA"
     gene            7120..8046
                     /locus_tag="VEW08_12605"
     CDS             7120..8046
                     /locus_tag="VEW08_12605"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HYJ41626.1"
                     /translation="MSSPFPPIIDISHAERAQTHREIDAACRETGLFQIAGHGIDTRL
                     ISALRRQARALFAEPTAAKLAISRTAENPWGYFDRERTRYMRDWKEVYDYGPADGEAI
                     APQWPSNLPAFKPVIQAYYEACDALAIRVLRIMARNLGMPPDYLDSHFRPQHTSFLRL
                     NYYPRCTGIQARHLGVNPHTDAGAITLLLQDEHPGLQVFHRGVWRPVEPHPELLVVFL
                     GDIAQVWSNDRYVAPWHRAVVHSRVERFSMPFYFNPVYSAEYAPLPSTVDARNPPRYR
                     PINWREFRSRRTAGDYANGREYLEISQYAVRR"
     gene            8124..10412
                     /locus_tag="VEW08_12610"
     CDS             8124..10412
                     /locus_tag="VEW08_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41627.1"
                     /translation="MTSNTRIRTLCLLALAALFSNASHAFNTIDVHVTINSSSTFSGD
                     WDEWWGKPDQFVAIYGDGTSAQAPACQSNTVINLMFVGPPAPADWRCDFNPQRPGKLY
                     IALYDGDGPNSNNTAGQQIDVAPGTAMAAMVNLETLISSPNTPFTFTSSGSQGTINFT
                     VTAQVNPGELLSFTPTMSAFRPSAGEQTVFTATATGAPYALLRVNAYNSAGANVWSLF
                     GYLDTPGQGLNGEASKTFVWQGRDANGYPLPPGIYTVKLQGFDVTTSLPAIGPLNGNG
                     IKTVGGMLVRTIQILAPPALPSLGVQGTIPAQKWAPDVGDLSINVVSSGTTQVVGEVF
                     AGTSCSGPTLQSLPAITVTPSFPGVLRWTGPLSPTMFPFGQYRIKVTGTSNGGPTSPA
                     FICQPIEMIAAPPASLFTQHLPFIPDPGSTVELTARSVDATGAPRIVGRLDVFGTVQS
                     VPGSPPAPPTAPLRTCTMASTCTTTISLPTGSSFISWRTTAADRAGMIADSGWRGQRV
                     TPPANFAQATSFAMPMDVSLTGPLMSNARNSANSMDLVFAVSTDFNWTSASDRQSIGD
                     ALDRFMSRLWGIEGIGAPAPTTFLARPDLVRVFVTPEQRTVSWNPNANLCDWTVPAVS
                     WADATAILHKTNCRDNAYTSSRSFSAQLFSRDVIMHELHHALFGLADEYPQGDGGYFE
                     AQPFPNVFNNVVDCLAVVGREPGGCTPIVERDVFTQMPTGRTFFRLDASVTDVMVGNG
                     TQRFGDIRQANFKEGVCDAGGC"
     gene            10391..10921
                     /locus_tag="VEW08_12615"
     CDS             10391..10921
                     /locus_tag="VEW08_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41628.1"
                     /translation="MRRRRLLVAGLGAAILGTGMASAPAAEEFSTEGLVSLESAGDGA
                     TLLVTGWLSREKFEVKEVRIAGSRLAGRSNDPDQFRVTLIDIAGREFGMVKMWSPLLS
                     LQWDAEGQKESAQGFNDRVVEIQIPVSWTLNEVVLSWPEGKYEVGRVSVRAEIERFCK
                     EMPDNPACRSAGRSKG"
     gene            11022..11891
                     /locus_tag="VEW08_12620"
     CDS             11022..11891
                     /locus_tag="VEW08_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYJ41629.1"
                     /translation="MKFYRIALIASAMCVFLAIGPGAIAAGQAATGPGVKSAASLVVD
                     GNNGEVIFEHDAGVVTPIASVTKLMTALVVLDGQQPLDEVITITAGDRWKGKGAFSHI
                     PNGAKLTRGDLLRLSLMASENLAARTLGRNYPGGMTAFVRTMNVKAKALGMTKTRFDD
                     PSGLSSLNVSTARDLVKLVNAAARDSRISDFSTLRSYEVRLGKRMFRYSNTNLLVGRP
                     DWEILVQKTGFTNDAGQCLVMEAMIGERPVIMVFLNSFGTLTRTADARRVRKWMEAKS
                     SPSVASVGAAAAK"
     gene            12015..12398
                     /locus_tag="VEW08_12625"
     CDS             12015..12398
                     /locus_tag="VEW08_12625"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ41630.1"
                     /translation="MLDHTGIVVSDLPKARKFYDAVAKSLGLGIVVPHKDFFLLGRGP
                     GQIPYLWIGTLRPSYWLEGSRAGINQMHVAFTAKDNARVDEFHRAAIAAGGRDNGKPG
                     PREAGYYGAFVLDPDGNNIEAVARG"
     gene            complement(12421..>13600)
                     /locus_tag="VEW08_12630"
     CDS             complement(12421..>13600)
                     /locus_tag="VEW08_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41631.1"
                     /translation="KARFAEARTAFEAGDFSRALFLYEQCLALGMQGPAVHYNIGVAA
                     YRADYLARAETAFQEVARTPAMAALAHYNLALVAQKRGDDKAARQWLERVALETTDEK
                     LVNLAQSQLDGLPKVATSPAWSLYARSGAGYDGNVALRSASIDTTGSGQEDAFGELML
                     AGSYSLRPSWRLDGAVGLLRYADLDEFNQDALSFGVVHGMTLNGWRLEPGVYLTQFSL
                     GGDVYERSAAASAEATRVFSGLGALRAQLRVSAVNGEGDFSGLTGSRSELGLKYEWEG
                     MQAWRFGAQARAEFNNSEDEAFASRWIELGADARWSASPRLAFVAGITLRQTRHPAQS
                     TTQDAWDDERTAFRLEATRLLWENLQLFVRYEHENNQSPIAENDYDRDWVAASIEYWR
                     "
BASE COUNT         2488 a         4354 c         4373 g         2385 t
ORIGIN      
        1 cgagttgttg tgcgagcgat ggactttgct cgtcatcagc cgcgtgatcg atggatgcac
       61 gcagttcaat gccattcacc gcggcgtgcc gcgcatctcc cccgcgatgc tcaccaaacg
      121 cctgcgccag ctcgaacacg ccgggctgct gacgagccga tcggtcggga agggcaagcc
      181 gcgaacgtac gcattgacgc cggccgggca ggagctctcg ccgctgatag atagtctcgc
      241 ggtgtggggg cagcactggg cgcgcgacat gtgcatggac gacctggatc cgggatttct
      301 tgcctggagc atgcacatgc gcctcgacgt cgccaggatg ccgccgggcc gcacggtgct
      361 cgcgttcgaa ttcaccgggg ccccgcgaga ctgccggcgt ttctggctgg tgaacgaaga
      421 tggctgcgtc gacatgtgtc tcaaggatcc gggctacgag gtcaatgtgc acgtccgttc
      481 cgatctgcgg cgtttcatcg aaacgtggcg cggcattcga gatctgcgcg ccgaaatacg
      541 tgcgcggcgc atcgagctca tcggtgcacc cgccctggtg cgaaagtttc cggattggct
      601 gaagctcagc ggccttgcgg tttttccacg ccagcgcccg gggcgggaga gccgtctggg
      661 ctagaacata accggtcgcg cctgaaactc cgacgtgccg aagtacattt ccaggccaat
      721 gcccacgccc cagttcgtaa tgcgtttttc cgtgagctct tcacgcggga aagtgcagct
      781 gatacgggtc tcgagcacca gccagtcccg cgcgacggct tgccgatacc ccagcttcac
      841 gccgtagttt tccgtcggca ccggtgcttc gaactcgccg gtggtgaaga tctgcgcggc
      901 gatcgcacgt cgattcggca ttccacgcat gacggtgagc gacgtgtaac cgcgcacgcc
      961 ttcggtttcc tgtgagatgg ttcccgaacc ggtccagcgt accagccagg ctttcacaac
     1021 ccgctcgacg tccatgcggc tggtgaagcc gaacccctcg ctgttctgcc agaaggcggt
     1081 ctcgcgaaag gtgtacagcg tttcctcgga agacccgcgc aggaagcgat agctgccctt
     1141 cacgaacgga tccagcggtg agcgaaagcg gatgccggcg tccgcctgga aattgccacc
     1201 ttgttttggc gagcgatatc ggatgccaaa caaggtttcg tcttcttccg cgggcccgta
     1261 ttgctgcgca aatgcgcccg atgccggagt gcgctcggta acgtactcct cgcgattcac
     1321 acggccgacg aaggcgtgca ggcgcgtgtt catgtgcggc agcggcacgt ccacgtcgaa
     1381 tcgcaggcgc ggctgcagtc cgtcgaattt atcccacagc agcgccggcg aaatcgagcc
     1441 gtttgcctgt tgatagagtg tttcgtcgcc gcgctgctca ccgaaccagc catcgacgcg
     1501 catcgccgag cgccacacgg cattccacac gccgaggtgc gcgcggtcga tgaacgggac
     1561 gacgaccggg tccgggattt cgtatgcgaa ttccggagcc ggatcgagct tggagcccgg
     1621 ctcaatgccg gcacccggat catcactcag aggatcgaga ttcgtgacgt cgaccgagat
     1681 gatcgtcgga tcgggaggcg tggtatcggc tgtcccgggc tcattcgtga tgacgggctc
     1741 ctgtgccagc gcgagcgttg gcagaagaat ccagcaaaca agattgaatc gtgaccatcg
     1801 ccacaccatg cggcctccac gataccagca tggcagttgt tggccatcga aaaggtcgga
     1861 cgattacccg gccctaggcg gacgattccc acaaaaagac gggggatccc ccaatggagc
     1921 gaggcgccgc gccaaatgcg cgtgttattt ctggcagcct gaaacaacgc ggcagggctc
     1981 cagcatgaca aacaaagtaa ccagaagcgc gatcgtcacc ggcgcttcgc gcggcatcgg
     2041 cgcggcgatc gccacgcggc tcgcggcgga tggtttcgcg gtcgtcgtca atttcgcggg
     2101 cagcgccggc gaagcggaaa aagtcgtcgg gcagattgag gcggcaggcg gcaaggccgt
     2161 tgcgattcag gccgacgtca gcagtggcga tgcgatgcgc cgcctgttcg atgagacgga
     2221 ggctgcgttc ggtggtgtcg atgtgctcgt caacaacgcc ggcatcatgc agctggcgaa
     2281 gttcgcggaa gcggacgatg cgttgttcga gcgtcaggtc gccgtgaacc tcaagggagt
     2341 tttcaacggc atgcgcgagg ccgcgaagcg attgcgttcc ggcggccgca tcatcagttt
     2401 ttcatcgagc gtggtcgggc tctatcaacc gacctacggc gtgtatgcgg ccaccaaggc
     2461 ggcggtggaa gccatgacgc atgtgctggc gaacgagatg cgcggccgga acattaccgt
     2521 gaatgcagtg gctcccggac ccaccgccac ggacctcttt ctgcacggta aaccgcagga
     2581 gctcgtggat cgcatcagca aggccgcgcc gctcgagcgc ctcggccagc cggacgacat
     2641 cgccgccgcc gtcgcgttcc tggccgggcc cgacggcgcg tggatcaacg gccaggtgct
     2701 gcgcgcgaac ggcggcctca tctagttagt cttcaccagc ggcggtcccg tgaattccga
     2761 ctcgagaatc acctcgactt catcgctcac acccatcgtg gtgccgggag caggaatgcc
     2821 gtggctcact ccaaagtccg agcgcttgaa ctggcccgtc gccgagaagc caacgcgagc
     2881 acgcggttca taggggtggc tcgcgtagcc gccgttgaag cgcgcgtcga gcacgatggc
     2941 cttcgtcaca ccgtgcagcg tgagatcgcc atggagtcga aacgagttct tgcccgtgac
     3001 ttcgacgttc gtcgagcgaa acgccatcgc ggggaatttt tcggcatcga gccagtcctt
     3061 gccggcgagt gagtcgagga agcccgacgg tgcgttgtcg gcctggatcg aacgcggatc
     3121 gatcgtcaca tttacgcgcg acgcagcaag gcgcgtggga tcgaagtcga gcttcgcgtc
     3181 gatacgcgtg aagcggcccg tataggtcga aaagcccagg tgacttacgc gaaacagaag
     3241 gctggtgtgc gcctggtcga tttgataggc gcctttgggg accggggcgg cggcgttctg
     3301 gtcgagcgcg gcggcgagcg gcgcataggc cgcgagcgtg agggcggcaa aagtggctaa
     3361 acgacgcatg atcctgactc ctcgagaatt tgtggggttc aggagttggt cgattggccg
     3421 gcccggaaat cgacacccaa aaacggtgtc aggcaccgtt ttctaggaaa cggtgccagg
     3481 caccgtttcc tagaaatcgg tgcctgaccc cagcgcgtcg tagggcgcgt gattgaatgt
     3541 gtactccgca agcacccgct ccgaggcgtc ctccacggcc agcagttgca tacgctttcg
     3601 ggcatgcccc atcgccctca gaacggccat gggaggcacg accgtgacca cgcctgacgc
     3661 ggcgtcgaac ggaatgtgtg cagatcgatg cggcgaacca ccgtcgaaat ccgcaaacac
     3721 gaggtccacc tggcgcacct cgccaagcgg cgcgtgcaga tacgagatca ccaggtcgtc
     3781 gtccggggcc accgtgcagt tgacgctctc tcccggtcgc agtgtgtatt cgcggatacg
     3841 cagaccatct gcctgcagcc tgcggacaaa ctccgctgac actaccgtgc cgacattgcc
     3901 actccgcagc tccctgcgca gcgcatcgcc gatgcgcacg agctcctgca gcgcggcgct
     3961 gcaagaggcg cactcgaaca ggtgttcttc gatcttcgcg gcggcggctt cgtccaactc
     4021 atccagccag taagcgagca gctccgcggt cgggtgttga tgctgattca ctgcgaaccc
     4081 tccgcttcca tgcagctgcg caggtgatcg agaccccgat ggcgaatcac gcgcacgtgc
     4141 ccggcgttga gtccgagctg cctgcccagc gcatcggcag ggcagtcgtc gtagaaggtc
     4201 atcaccagca ccgcgcgctc acgctcgggc agggcctgca ggcagtggcg aagacgttgc
     4261 tcatccaggg gttcggtcgc cgacgcttgg tcgggaatga ggtcgactgg aaaggtatcg
     4321 aggatgcgct cgcgccggcc gctgctgcgc cgcgagtcga ccatcatctg ccgcgcggtg
     4381 cccagcatga acgaagcgat gcgttccgga tctcgtaccg cgccggcgcg cagcttctgc
     4441 agcgccatga gcaacacgtc ctgcaccagg tcagccgccg cggcttcgct gcgcagccat
     4501 ttgcggccaa acaaccggat gcgcggcgcg aagcggcggc agaactcggc ctcggcgccg
     4561 gtgtcggagg cggcgatgcg ccgcgcgagt gaggcgtcgt cgtcctgcgt ttcagccacg
     4621 tattgcacgg gcgaagcata aatttcgcgc gcgacgatcg aaagtacccg gttcatggag
     4681 agggtgtgta acggggaggc cggtcgttac acatcctcga gacctgcaaa tgggagttcc
     4741 gatgatcacc aacacacagt ccggcaccaa catccaggaa gtggcggacg gcatctatcg
     4801 aatcaacacc cccgtggcca ttccgggcgc gggcgacttc agtttcaacc agtacctgct
     4861 cgtggacgat gcaccgctgc tgtttcatac cggccagcgg cagttgttcc cgctggtaag
     4921 cgaggccatt cgggcggtca tgccgctcga caagctgcgt tacctggggc tgtcgcactt
     4981 cgaggccgat gagtgcggcg cactgaattc gtttctggcc gtggcgcccc aggccgttcc
     5041 ggtgtgcagc caggttgccg ccatggtggc gatcaacgat tttgcggatc gggcgccgca
     5101 ggcgctggcc gatggagcag aactctcact cggccggcac acggtgcgct ggttcgacac
     5161 gccgcacatg ccgcactcct gggaatgcgg gttgatgatg gagatgacca cgcggacctt
     5221 cttctgcggc gatctgttca cgcagggtgg aaagggcgag gcggcgctga ccgagtccga
     5281 catcctggga ccgagcgaag ccttccgcgg tcccatggac tactacgcgc atgcgcccca
     5341 aaccaaggcc acgctcgagc ggctggcgcg tgagcagccg acgacgcttg cggtgatgca
     5401 cggcagctcg tggcgcgggg atggagcgaa gttgcttcgg gagctttcgg tgaagctcgc
     5461 ggcgacgcgg cactaggacg ggaagggtgt caggcaccgt ttcctagaaa acggtgcctg
     5521 gcaccatttc ctagaaaacg gtgcctgaca ccatttttcc cacgccggct cccacggtgg
     5581 gccttcagaa ttcctccatt ccaaggagat acctatggcc cgttaccaga acattctcga
     5641 aaccatcggc aacacgcccg tggtcaggct caacaagctc gctccgcccg gggtgaacgt
     5701 ctacgtcaag gtggaatcct tcaacccgat gggttcggtc aaggaccgca tggcgcgcgc
     5761 catcatcgag gacgccgagc gcagcggcgc gttgcttccg ggccagaccg tcatcgaagc
     5821 caccagcggc aacaccggca ttggcctggc gatggtgtgt gcgcagaagg gctatccact
     5881 ggtcgtcacc atggccgaaa gtttcagcgt cgagcgccgc aagctgctcc gctttctcgg
     5941 cgccaaggtg gtgctgacgc ccgcggccga gaagggcagc ggcatgctcg cgaaagccgt
     6001 cgaactcgcg atgacgcacg gctggttcct gtgccggcag ttcgagaacc cggcgaatgc
     6061 ggacgtgcac tcgcgcacca cggcgcgcga gatcatcaac gacttttcgg atcagcggct
     6121 cgactacttc gtgactggct tcggcaccgg cggcacgctg aaaggcgtgg cgcgtgaact
     6181 gaagcgcgcg cgaccgggca cgcggatcat cgccgccgag cccgacaact cgcaggtctt
     6241 gggcagccgc atcccgcaga ttcgcgacac cgccggcggc cccgcgggaa gccacccgcg
     6301 ttttcgcccg catctcatgc agggctggtc gccggatttc atttcgacgc tgaccgaaca
     6361 ggcggtggaa gccgggttga tcgacgagat cgtgccggtc gcaggcgccg aggcgctgcg
     6421 cctctcgcgc gagctcgcgc ggcaggaagg aatcttcgtc ggcacctcga gcggcgccac
     6481 gctcgcggcg gcactcgacg tcgcgcggcg cgcgccggcc ggctcgacca tcgtgtgcat
     6541 gctgcccgac accggtgagc gatatctctc gacgccgctg ttcgaaagca tcggtgaaga
     6601 aatgaccgcc gaggagtggg agctttcgcg ctcgacaccg ctgtgccggt tcgatgcgcc
     6661 atccgcgccg gcgccggccc cggccgccgc tgagcaaaag gtcgcggtcg accccgccgc
     6721 ggcgcgtttc gtcaccgaag ttctcgaaaa ggaacccgtc gtgatgttcg cgctcgagtg
     6781 gtgtgagttc tgctggtcgg tgcgcaagct gttcacgaag ctcggcatcg cctatcgcag
     6841 cgtcgatgtc gactccgtcg aattccagaa agacgacctc ggcggcagga ttcgcggcgt
     6901 tctcgcggat cgcaccggcg cgaagactct gccgcaggtg ttcgtgggcg gcgagcacat
     6961 cggcggcgcc accgatacct tcgacgcctg gcggaacggc acgctgcagg gccggctgga
     7021 taagaaagga gtgagctacg accgcgaagc gaagctggat ccttactcgc tgctgccgaa
     7081 atggctgcag ccgcgcaagc aggcctagtg gagcggatga tgagcagtcc ctttcccccc
     7141 atcatcgaca tttctcacgc cgagcgggcg cagacacacc gcgagatcga cgccgcttgc
     7201 cgcgaaacgg gcctgtttca gatcgccgga catggcatcg atacgcgcct catctccgcg
     7261 ttgcgccgcc aggcgcgtgc gttgttcgcg gagcccaccg cggcgaagct ggcgatctcg
     7321 cgcacggccg agaatccctg gggctatttc gaccgcgagc gcacccgcta tatgcgcgac
     7381 tggaaagagg tctacgacta cggccccgcg gatggcgaag ccatcgcgcc gcagtggcct
     7441 tcgaatctcc cggcgttcaa gcccgtgatt caggcgtact acgaggcctg cgatgcgctc
     7501 gcgatccggg tgctgcggat catggcgcgc aatctcggaa tgccgccgga ctacctggac
     7561 tcgcatttcc gcccgcagca cacgagtttc ctgcgcctga actactaccc gcggtgcacg
     7621 gggattcaag cgcgccatct cggcgtcaat ccccacactg acgccggcgc gatcacactg
     7681 ctgttgcagg acgagcatcc cgggctccag gtctttcacc ggggtgtgtg gcggcctgtc
     7741 gagccgcatc cggagttgct ggtcgtgttt ctgggcgaca tcgcgcaggt gtggtccaac
     7801 gatcgctacg tggctccatg gcaccgggcg gtggtgcact ccagggtgga acgtttcagc
     7861 atgccgttct acttcaaccc ggtgtacagc gccgaatacg cgccgctgcc gtcgaccgtg
     7921 gatgcgcgga atccgccacg ttatcgcccg atcaactggc gcgagttccg gtcgcgccgc
     7981 acggccggcg actacgccaa cggccgcgaa tacctggaga tcagccagta cgccgtcagg
     8041 aggtagttcg gcgatgcagc gtgatctccg gagtcatccg tttccgctag atggggtagg
     8101 gaccccactg ccggagaaaa ggaatgactt cgaacacgag aataaggacg ttgtgtcttc
     8161 tggcgctggc cgcgcttttc agcaatgcca gccacgcctt caacaccatc gacgtgcacg
     8221 tcaccatcaa ttcgtcgagt accttcagcg gagactggga cgagtggtgg ggcaagcccg
     8281 atcaattcgt cgccatctat ggcgacggga ccagcgcgca ggcgcctgcg tgccagagca
     8341 acaccgtcat caacctgatg ttcgtgggcc cgccggcacc ggcggattgg cgctgcgact
     8401 tcaaccccca gcgccccgga aagctgtata tcgcgctcta cgatggagat ggccccaact
     8461 cgaataacac ggcgggccag cagatagacg tggcgcccgg cacggccatg gccgccatgg
     8521 tcaatctcga gaccttgatc agcagtccta acactccgtt cacgttcacg agctcgggca
     8581 gccagggcac gatcaacttc acggtcactg cgcaggtcaa tcccggcgaa ctgctctcgt
     8641 tcaccccgac catgagcgcg ttccggccgt ccgccggtga gcagaccgtg ttcacggcca
     8701 cggccaccgg tgctccctac gcgttgttgc gcgtcaatgc gtacaacagc gcgggcgcca
     8761 atgtgtggag cctcttcgga tacctcgaca ctcccgggca gggcttgaac ggggaggcct
     8821 cgaagacatt cgtgtggcag ggccgcgatg ccaacggata tccgctgccg cccgggatct
     8881 acacggtcaa actacaggga ttcgacgtca ccaccagtct gccggcgatc ggccccttga
     8941 atggcaacgg catcaagacc gtgggtggaa tgctcgtgcg gactatccag atcctggcgc
     9001 ctccggcgct gccctccctc ggagtgcaag ggaccattcc cgcgcagaag tgggcgcccg
     9061 atgtcggcga cctgtctatc aatgtcgtca gcagcgggac aacgcaggta gtcggcgaag
     9121 tgttcgccgg cacgagctgc agcggcccca ctctgcagag cctcccggcg atcaccgtga
     9181 cgccgtcatt cccgggcgtg ttgcgatgga ccgggcctct cagccccacg atgtttcctt
     9241 tcgggcaata ccgcatcaag gtgactggca cctcgaatgg cggccccaca tcgcccgcgt
     9301 tcatctgcca gccgatcgag atgatcgcgg caccgccggc cagcttgttc acacagcact
     9361 tgccgttcat ccctgacccg gggagcacgg tggaactcac cgcgagatcc gtcgatgcaa
     9421 ccggcgctcc gcgcatcgtg ggcaggctgg atgtgtttgg cactgtgcaa tcggttccgg
     9481 gctcgccgcc ggcgccgccg acagcgccgc tgcggacctg caccatggcc tctacatgca
     9541 cgacgacgat ctcgttgccc accggcagct cgttcatcag ctggagaacg acggccgcgg
     9601 atcgcgccgg catgatcgcg gattccggct ggcgcggaca gcgcgtcacg ccgcccgcga
     9661 atttcgctca agccacgagt ttcgcgatgc cgatggacgt ctctctcacc gggccactca
     9721 tgtcgaatgc gcgcaacagc gcgaacagca tggacctggt gttcgcggtg tcgacggact
     9781 tcaactggac cagcgcgtcc gaccgccaat cgatcggcga tgcgctcgac cggttcatga
     9841 gccggctgtg gggcatagaa ggcattggtg ctcccgcgcc gactacgttc ctggcgcggc
     9901 ccgatctggt gcgggtgttt gtgacgcccg agcagcgcac cgtgagctgg aacccgaacg
     9961 cgaatctttg cgactggacg gtgccggcgg tgtcctgggc ggatgccacg gcgatcctgc
    10021 acaagacgaa ttgccgcgac aacgcctaca cctcgagccg gagcttctcc gcgcagttgt
    10081 tttcgcggga cgtgatcatg cacgagctgc atcacgcgct gttcggactg gccgatgaat
    10141 atccgcaagg cgacggcgga tacttcgagg cacagccatt cccgaacgtc ttcaacaacg
    10201 tggtcgactg cctggcggtg gtcggtcggg agccgggcgg ttgcacgccg atcgtcgaga
    10261 gggacgtctt cacacagatg cccaccgggc ggacattctt ccgtctcgac gccagcgtga
    10321 cggacgtgat ggtaggaaat gggactcaac gattcggcga tattcgccag gctaacttca
    10381 aggagggtgt atgcgacgca ggaggttgtt agtagcgggc ctgggggccg cgattctggg
    10441 cactggcatg gcgagcgcgc cggcagcgga ggaattctcg accgagggac tcgtatccct
    10501 cgagtcggca ggagacggag ccacgttgct cgtgaccggc tggctgtcgc gagagaagtt
    10561 cgaggtgaag gaggtgcgca tcgccggttc gcggctcgcg ggaagaagca acgacccgga
    10621 tcagttccgg gtcaccctga tcgacatcgc ggggcgcgag ttcggcatgg tgaagatgtg
    10681 gtcgccgctg ctctcgctgc agtgggatgc ggaagggcag aaggaatccg cccagggttt
    10741 caacgatcgc gtagtggaga tacagatccc cgtgtcgtgg accctgaacg aagtcgtcct
    10801 gagctggccc gaaggcaagt acgaagtcgg gcgcgtgtcg gtccgcgccg agatcgagcg
    10861 cttctgcaag gagatgccgg acaaccctgc ctgccgcagc gccggcagat cgaaaggctg
    10921 aaaaaggggt caggcaccgt ttcctaggaa acggtgcctg acaccatttt ctaggaaacg
    10981 gtgcctggca ccattttcgg ttccgtccta ccatggcggc catgaagttc taccggatcg
    11041 cgcttatcgc gagtgcaatg tgtgtattcc tcgcgatcgg gcccggcgcg atcgcagccg
    11101 gccaggccgc aaccgggccg ggcgtcaaat ccgcagcctc actcgtagtg gacgggaaca
    11161 acggcgaggt gatcttcgag cacgacgccg gcgtcgtgac gcccatcgcg tcggtcacca
    11221 agctcatgac cgcgctcgtc gtgctcgacg gtcaacagcc gctcgatgaa gtcatcacga
    11281 tcacggccgg cgatcgctgg aaaggcaaag gcgccttctc gcatattccc aacggcgcaa
    11341 agctgacgcg cggcgacctg ctgcgccttt cgctcatggc atcggagaac ctcgccgcgc
    11401 gcacgctcgg gcgcaactac cctggcggca tgaccgcgtt cgtccgcacg atgaacgtga
    11461 aagccaaggc gctcgggatg acgaagaccc gcttcgacga tccgtccggc ctctcgagtc
    11521 tcaacgtgtc gactgcgcgg gacctggtga aactcgtcaa cgcagcggcg cgcgactccc
    11581 ggatcagcga tttctcgacg ctgcgcagct acgaggtccg gctgggcaag cgcatgttca
    11641 ggtatagcaa caccaacctg ctcgtcggca ggccggactg ggaaatcctg gtgcagaaga
    11701 ccggcttcac caacgatgcc ggtcagtgcc tcgtgatgga agcgatgatc ggcgagcgcc
    11761 ccgtcatcat ggtgttccta aactcgttcg gaacgttgac gcgcaccgcg gatgcaagac
    11821 gcgtccgcaa gtggatggaa gcgaaaagtt cgcccagtgt cgcgagcgtc ggggcggcgg
    11881 cggcaaaata gagagagtgg gacatcgctc gcccgctctg tcagctcgtt ccgacgtgta
    11941 atggcgctgg tccacgcatt cagcgaaccc cggcacgcac gtagtcttgg gggcccgaac
    12001 cactggagcc gcccatgctc gatcacactg gcatcgtcgt gtccgatctt cccaaggccc
    12061 gcaagttcta cgacgcagtc gcgaagtcgc tgggactcgg gatcgtcgta ccccacaagg
    12121 acttcttctt gttaggccgc ggcccgggac aaattccgta tctgtggatc gggaccctga
    12181 ggccgtcgta ttggctggaa ggctcgcgcg ccggcatcaa ccagatgcac gtggcgttca
    12241 ccgcgaagga caacgcgcgc gttgacgaat ttcaccgtgc agctatcgcc gcaggtggcc
    12301 gcgacaacgg caaaccgggt ccgcgcgaag cgggctacta cggcgctttc gtgctggacc
    12361 cggacggcaa caacatcgag gccgttgcgc gcgggtgagc tcccatctcc atttccggga
    12421 tcagcgccag tattcgatcg atgccgccac ccaatcacgg tcgtagtcgt tctccgcaat
    12481 cggcgattgg ttgttctcgt gctcatatcg cacgaatagc tgcaggtttt cccaaagcag
    12541 ccgtgtcgct tccagccgga acgcggtccg ctcgtcgtcc caggcgtcct gcgtcgtcga
    12601 ctgcgccgga tgtcgtgtct ggcgcagcgt aatacccgcc acgaaagcca gccgcggtga
    12661 ggctgaccac cgtgcgtccg cgcccaactc gatccatcgc gacgcgaacg cttcgtcttc
    12721 gctgttgttg aattccgcgc gagcctgcgc tccgaagcgc caggcctgca ttccttccca
    12781 ttcatacttc aacccgagct cggagcgaga acccgtgagg ccgctgaagt cgccttcacc
    12841 gttgacagcg ctgacacgca gctgcgcgcg caacgctcca agaccggaaa aaacgcgagt
    12901 cgcctctgcc gacgcggccg cggatctctc atagacatca ccgcccaggg aaaactgcgt
    12961 gagatagacg ccgggttcca ggcgccatcc gttcagcgtc atgccgtgga ccacgccgaa
    13021 tgacagcgcg tcctggttga actcatcgag gtcggcgtag cggagcaatc ccacggcgcc
    13081 gtcgagacgc caggacgggc ggagcgaata actgcccgcg agcatcagct caccgaatgc
    13141 gtcttcctgt ccgcttccgg tggtatcgat cgacgccgag cgcaatgcca cgttgccgtc
    13201 gtagccagcg ccgctgcgcg catacagcga ccaggccggg ctcgtcgcaa ccttcggcag
    13261 cccgtcgagc tggctctgcg ccaggttcac gagtttctcg tcggtggtct cgagtgccac
    13321 gcgctctaac cactggcgcg cggccttgtc atcgccgcgc ttctgcgcga ccagggccag
    13381 gttgtagtga gccagcgccg ccatggcagg tgtgcgggcg acctcctgaa atgcggtctc
    13441 ggcgcgcgcc aggtaatctg cgcgatacgc cgcgacgcca atgttgtagt gaactgcggg
    13501 tccctgcatt cccaacgcca gacattgctc gtacaggaac aaggcgcggg aaaaatctcc
    13561 ggcttcgaat gccgtgcgcg cttcggcgaa gcgtgccttg
//
LOCUS       DASQJS010000313         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1758916, whole genome shotgun sequence.
ACCESSION   DASQJS010000313 DASQJS010000000
VERSION     DASQJS010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758916"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VEW08_12635"
     CDS             complement(<1..299)
                     /locus_tag="VEW08_12635"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HYJ41632.1"
                     /translation="MPTSIPTLDARNAEGPQFANDIAAAYAEFGFVIIENHGIDKALI
                     DRCLDCFHRFFALPVAEKLRYQVPGGGGARGYTAFGIETAKGAKHHDLKEFWHVGR"
     gene            404..1312
                     /locus_tag="VEW08_12640"
     CDS             404..1312
                     /locus_tag="VEW08_12640"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HYJ41633.1"
                     /translation="MVRPKHFAYNAQTAGTNRFQNPEGRPGALDVAHQALREFDAFAA
                     ALTAEGVKVCVVPDSDTPRKPDAVFPNNWVSFHADGTVVLYPMHAENRRAERRPEIIE
                     AVTRETGFQVRRVLDLTRHENEGRFLEGTGSLVLDHRARVAYACRSPRTDESVAREWA
                     REMGFELELFTATDESGTPIYHTNVLMYVGTRVAVVAVDAIAPADRARVSGRLVATGL
                     DVIAIDHAEMRAFAGNMLEVGSWDEYLGDFTILVMSETARHALATRKYIRLYSSVDAV
                     LALPIDVIERHGGGGVRCMLAEIFLP"
     gene            1346..1993
                     /gene="plsY"
                     /locus_tag="VEW08_12645"
     CDS             1346..1993
                     /gene="plsY"
                     /locus_tag="VEW08_12645"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HYJ41634.1"
                     /translation="MEFPVKILIAYLLGSIVGSLVVGRLRGGVDIRQAGSGNAGATNA
                     LRTQGAAFAIWVILIDIAKGWFAAALLPGLALPGIAPASAPLAVWLPAVCGFVAILGH
                     VFPVWHGFRGGKGVATLVGAFAGIDLALLVPLFASWLAVVTVSGFVGLASITAAIAIP
                     VYLLLRDGPVLTPVLGFALACAVLVLYTHRGNVRRMREGTEPRARKLWLFGRGRA"
     gene            1990..>2184
                     /locus_tag="VEW08_12650"
     CDS             1990..>2184
                     /locus_tag="VEW08_12650"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HYJ41635.1"
                     /translation="MSAAASTQAPLPQRVFQRLDDRSFVSGEALAADLSVTRAAVWKA
                     VEQLRELGVALDAQTNKGYRL"
BASE COUNT          329 a          713 c          732 g          410 t
ORIGIN      
        1 cggcccacgt gccagaactc cttgagatcg tggtgcttgg cgcccttggc ggtttcgatg
       61 ccgaacgccg tgtagccccg cgcaccgccg ccgcccggca cctggtagcg gagtttctcg
      121 gccacgggca gggcgaagaa ccggtggaag cagtccaggc agcgatcgat caacgccttg
      181 tcgatcccgt ggttttcgat gatcacgaac ccgaactccg cgtacgcggc cgcgatgtcg
      241 ttcgcgaact gcgggccttc ggcgttacgc gcgtcgagcg tgggaatgga agttggcatc
      301 gagggctatt gtgcctaccg ggtccgggtt gtagaagacg gtaatgcagg gtgggaaaga
      361 acggggccga tcaggcccct cgcagtgtgc aagcgccata ttgatggttc gcccgaaaca
      421 cttcgcatac aacgcgcaaa ccgctggcac caatcgattc cagaatcccg aggggaggcc
      481 cggcgccctg gatgtggcac accaggcatt gagggaattc gacgccttcg ccgcggcgct
      541 gaccgccgag ggtgtgaagg tgtgcgtcgt gccggattcg gataccccgc gcaagcccga
      601 cgccgtgttt ccgaacaact gggtcagttt tcacgccgac ggcactgtgg tcctgtaccc
      661 catgcacgcc gaaaatcgcc gcgcggagcg gcgtcccgaa atcatcgagg ccgtgacgcg
      721 cgaaaccgga tttcaggttc gccgcgtgct cgacctgact cgccatgaga atgaagggcg
      781 gttcctcgaa ggcaccggca gcctggtgct cgaccaccgc gcgcgggttg cctacgcctg
      841 ccgctcgccg cgcaccgacg agtcggtggc gcgcgaatgg gcgcgcgaga tgggtttcga
      901 gctcgagttg ttcaccgcca ccgatgaatc cggcacgccc atctaccaca ccaatgtgct
      961 catgtacgtg ggtacgcgcg tcgcggtggt ggccgtcgac gcgattgccc ccgccgaccg
     1021 cgcgcgtgtg agcgggcgcc tggtcgctac gggcctcgac gtgatcgcga tcgatcacgc
     1081 ggaaatgcgc gcattcgctg gcaacatgct ggaagtgggc agctgggatg agtacctggg
     1141 cgatttcacc atcctggtga tgtcggaaac cgcgcgccac gcgctggcca cccgcaagta
     1201 catacgcctc tattcatccg tggatgcggt gctcgcgctg ccgatcgacg tgattgaacg
     1261 tcacgggggc ggcggtgtcc gatgtatgct cgcggaaatt ttcctcccct gaagtttcgc
     1321 ccccgtccct gatcaatcgg ctcccatgga atttccggtc aagatcctca tcgcctatct
     1381 tctcggctcg attgtcggaa gtctggtcgt gggccgcctg cgcggcggcg tcgacatccg
     1441 ccaggcgggc agcggcaatg ccggggccac caatgctctg cgcacgcaag gcgcggcgtt
     1501 cgcgatctgg gtcatcctca tcgatatcgc caagggctgg tttgccgcgg cattgctgcc
     1561 ggggctggcg ctgcccggaa tcgcgcccgc gtccgccccg cttgccgtgt ggctgccggc
     1621 ggtctgcggg ttcgtcgcca tcctcggcca cgtctttccg gtgtggcatg gatttcgagg
     1681 cggcaagggt gttgccacgc tggtgggcgc attcgccggc atcgacctgg ccctgctggt
     1741 gccgcttttc gcgagctggc tcgcggtggt cacggtgtcc ggtttcgtcg ggctggcatc
     1801 gatcaccgcc gctattgcca ttcctgtcta tctactgctg cgtgacggcc ccgtgctgac
     1861 tccggtgctc gggtttgcgc tggcgtgtgc cgtgctcgtg ctctacaccc atcgcggcaa
     1921 tgttcgccgc atgcgggaag gcaccgaacc ccgcgcgcgc aagctctggc tgttcggacg
     1981 cgggcgcgcg tgagcgccgc cgcctccaca caggcgccgc tgccgcagcg cgtgttccag
     2041 cgcctcgacg atcgcagctt cgtctccggc gaagcgctgg cggcggacct gtcggtgacg
     2101 cgcgccgccg tctggaaagc ggtcgagcaa ttgcgcgagc tcggcgtggc gctggacgcg
     2161 cagaccaaca agggttatcg gctc
//
LOCUS       DASQJS010000314         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2389661, whole genome shotgun sequence.
ACCESSION   DASQJS010000314 DASQJS010000000
VERSION     DASQJS010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2389661"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="VEW08_12655"
     CDS             complement(<1..1307)
                     /locus_tag="VEW08_12655"
                     /inference="COORDINATES: protein motif:HMM:NF044822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41636.1"
                     /translation="MTGPLIAVFDVGKTNAKLAVVDPALGQEIWSVRRANNVVQGPGG
                     RELDVVAIEAWLLESLRAAPQRERIAVIVPIAHGAAAVLVDHAGEVLAAPDYEDPCFD
                     EVSAEYVPLRDNYSLTFSPSLPQGLNLGRQLFYLQTRRPTLFQRAAHLLLYPQYWAWR
                     LSGTMAAEVTSLGTHTDLWRPHEHTYSTLARRQGWARMMPSQHPANARLGCIRSGVAA
                     ATGLGGDCAVACGIHDSNASYLRFLMDREREAFTVVSSGTWTIVMANRGDLRRLREDR
                     DMLANVNAFGAPVATARYMGGREYEAVAQSGDEPTVDAVIEVLSLGAIALPSFASAGP
                     FSGRPGRLEGVDTLHGTQRAALATLYSALMTAILIESLGAAGEIFVDGPLARNPLFSR
                     LLAACVPVGTVRTYPEGGGTRVAAHLAGLPSPAPGALQSVVPLR"
     gene            complement(1304..2251)
                     /locus_tag="VEW08_12660"
     CDS             complement(1304..2251)
                     /locus_tag="VEW08_12660"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ41637.1"
                     /translation="MRPANLLRWESMLVLVLVLEFIVFSTISPYFLSVDTLSDATFNF
                     TERAIVALPLALLIIAGEIDISVAAIIALASVAMGLASAQGMGTAGIAGVGLSTGLLA
                     GCINGALVTIGRVPSIVATIGTMTLFRGAAYAVLGDRVLKDYPESFAAFGQGYFAGPV
                     SIELALFALFALSFGVFLHRTRWGRTIFAIGANPVASRFAGVTVNRYRFVLFALTGLF
                     SGLAAVLLTSRLGSTRPSIAQGWELEIISMVILGGVSVWGGKGSILGVVLAAAVFGLV
                     TFGLGLMNIPGIVMSIFTGTLLILVVAVPALVNRPLVNK"
     gene            complement(2248..>2679)
                     /locus_tag="VEW08_12665"
     CDS             complement(2248..>2679)
                     /locus_tag="VEW08_12665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41638.1"
                     /translation="LRRSRVARDLYAAGGNPEAANYSGIDAGRMQFIAYTICGAIAGV
                     CGYLWVARFAVAYTDIALGFELSVIAACLIGGVAIAGGVGSAVGVVLGCLFIGIIRSS
                     LPLVGISAFWQMFINGVVILVAVLLSARQREYKRTILGVRT"
BASE COUNT          554 a          923 c          832 g          370 t
ORIGIN      
        1 cgcagaggta ccacggattg cagcgcgccc ggcgccgggg atggcaaccc ggccaggtgc
       61 gcggcgaccc gcgtgccgcc gccctccgga taagtgcgca ctgttcccac cggcacgcac
      121 gcggctaaca acctggaaaa cagcggattc cgcgccagcg ggccatcgac gaagatttcg
      181 ccggccgcgc ccagcgactc gatcaatatc gccgtcatca gcgccgaata gagcgtggcc
      241 agcgcggcgc gctgcgtgcc gtgcagcgta tcgacgcctt cgaggcgccc gggccggccg
      301 gagaacgggc cggcgctggc gaacgaaggc agcgcgatcg cacccagcga caatacttcg
      361 atcacggcat cgaccgtagg ctcatcgccg ctctgcgcga ccgcttcata ctctcgtcca
      421 cccatgtagc gtgccgtggc cacgggtgcg ccgaacgcat tgacgttggc gagcatgtct
      481 cgatcctcgc gcagacgcct gaggtcccca cggttcgcca tcacgatggt ccaggtgccg
      541 ctcgacacca ccgtgaacgc ctcgcgctcg cgatccatga gaaagcgaag atacgaggcg
      601 ttggaatcgt gaatgccgca cgccactgcg cagtccccgc cgagtccggt cgccgcggcc
      661 acgccggacc ggatacagcc gagacgcgcg ttggcgggat gttgcgacgg catcatgcgt
      721 gcccatccct gcctgcgcgc gagcgtcgaa taagtgtgct cgtgcgggcg ccacaaatcg
      781 gtgtgggtgc cgagcgaggt cacttcggct gccatcgttc ccgacaggcg ccacgcccag
      841 tactgcggat acagcagcaa gtgcgcggct cgctgaaaca gcgtgggacg acgcgtctgc
      901 agatagaaca gctggcggcc gagattgagg ccctgcggga gactcggcga gaaggtgagc
      961 gaatagttgt cgcgcagcgg cacgtactcc gcgctcacct cgtcgaaaca cggatcttcg
     1021 tagtccggcg ccgccagcac ttcgccggca tgatcgacca gcaccgcggc cgcgccgtgt
     1081 gcaatcggca caatcaccgc gatgcgctcc cgttggggag cagcgcgcag ggactcgagc
     1141 agccacgctt cgatcgcgac gacatcgagc tcgcgccctc cgggaccctg caccacattg
     1201 ttagcccggc gcacgctcca gatttcctgg cccagcgccg gatcgaccac cgccagcttg
     1261 gcgttggtct tgcccacgtc gaatacggca atgagcggac ccgtcatttg tttaccaagg
     1321 gacggttcac cagcgccggc accgccacca ccaggatgag cagagttccc gtgaagatcg
     1381 acatcacgat gccggggata ttcatgagcc cgagcccgaa cgtcacgagg ccaaacaccg
     1441 ccgcagcaag cacgacaccg aggatgctgc ccttgccacc ccagaccgat acgccaccga
     1501 ggataaccat gctgatgatc tcgagctccc agccttgcgc gatcgagggc cgcgtcgacc
     1561 ccaggcgcga tgtcagcagc accgcggcaa gtccgctgaa gaggccagtc aacgcaaaca
     1621 gcacgaatcg ataacggttc accgtgaccc ccgcaaagcg gctagccacc ggattcgcgc
     1681 cgatggcgaa gatggtgcgg ccccagcgtg tgcgatgcag aaacacgccg aacgataggg
     1741 cgaagagcgc gaacagcgcc agctcgatgg aaaccgggcc ggcgaaatac ccttgtccaa
     1801 aggcggcgaa cgactccggg taatccttca gcacccgatc cccgagcacc gcatacgcgg
     1861 cgccgcggaa cagcgtcatg gtgccgatgg tggcgacgat ggacggcact cgtccgatcg
     1921 ttaccagcgc accgttgatg catccggcca gcaatccggt gctcaagccc acgccagcta
     1981 tgccggcggt gcccatgccc tgtgccgacg ccagccccat ggccaccgac gcgagcgcaa
     2041 tgatggcagc cactgaaatg tcgatctcac cggcaatgat cagaagcgcc aacggcaacg
     2101 cgacgattgc gcgctcggtg aagttgaacg tcgcatccga cagcgtgtcg acgctcagga
     2161 aatacggtga gatggtcgag aacacgatga attcgagcac cagcaccaga accagcatcg
     2221 attcccagcg aagcagattc gccggcctca tgtgcgcacc ccaagaatgg tgcgcttgta
     2281 ctcgcgctgc cgggcgctca gcagcacggc aaccaggatg accacaccat tgatgaacat
     2341 ctgccagaag gccgagatac ccacgagcgg caggctgctg cgaatgatgc cgatgaacag
     2401 gcaccccagc accacgccca ccgccgaacc gacgccaccg gcaatcgcca cgccaccgat
     2461 gaggcacgca gcgatgaccg aaagctcgaa gccgagcgcg atgtcggtgt acgccaccgc
     2521 gaagcgcgcc acccacaggt agccgcagac gccggcgatg gcgccgcaga tcgtgtatgc
     2581 gatgaactgc atgcgtcccg catcgatgcc ggagtagttt gccgcctcgg gattgccgcc
     2641 ggccgcgtac aggtcgcgcg cgacacgtga gcggcgcag
//
LOCUS       DASQJS010000315        19165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4270388, whole genome shotgun sequence.
ACCESSION   DASQJS010000315 DASQJS010000000
VERSION     DASQJS010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19165
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4270388"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..2206)
                     /gene="uvrB"
                     /locus_tag="VEW08_12670"
                     /pseudo
     CDS             complement(278..2206)
                     /gene="uvrB"
                     /locus_tag="VEW08_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
     gene            complement(2213..4006)
                     /locus_tag="VEW08_12675"
                     /pseudo
     CDS             complement(2213..4006)
                     /locus_tag="VEW08_12675"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
     gene            4111..5316
                     /locus_tag="VEW08_12680"
     CDS             4111..5316
                     /locus_tag="VEW08_12680"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HYJ41639.1"
                     /translation="MPNQLSRRVQRVKPSPTLAVTARAAKLKAEGKDIIGLGAGEPDF
                     DTPAHIAEAGIDAIRKGFTRYTNVDGIVELKDAIIAKFKRDNQISYERNQILVSSGAK
                     QTIYNLCMAVLDPGDEAVIPAPYWVSYPDMVLLADGLPVTPICPASQGYKLTPRQLEA
                     AITPKTRLLLINSPCNPTGAAYTRAEWRALADVLVQHPRVIVGTDDMYEKIWWGPEPF
                     SSLLTVAPELYDRVVTINGVSKSYAMTGWRLGYCGGPKEIVNAMSTIQGQSTSNASSI
                     TQKAALAALNGDQKPVELMNIEFKKRHDWFIGALNRLPGIVALPCAGAFYAWADCSAA
                     MMAFGIRDDNAFAEMLINDAGVAVVPGSGFGAPGHFRASFATSMAVLEQSIERMKKVL
                     EAGPVKKSA"
     gene            5339..6361
                     /locus_tag="VEW08_12685"
     CDS             5339..6361
                     /locus_tag="VEW08_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYJ41640.1"
                     /translation="MIPAAPTPITRADYTAWILVALALGLVIELHLLSGLLAGLLVFK
                     LVDVLTPWLRLRALGRDGPRVLAVTLIATAVIAALIGIGIGLATLMRNSGESVPLLME
                     KMAQIIEDARAILPASLQKYVPEDSATLRRTLAEWLRSNAGALQLAGRGIGRSVVHVL
                     MGMIIGALLSMQKAAHPHDRRPLTEHIVRHAARFATAFQRVVFAQFWISLINTFFTWL
                     YLDVVLRLFGIDLPLVKILVALTFIVGLLPIIGNLISNTAIVVVCLSQGVPVAAASLV
                     YLVVIHKLEYFLNARIIGSHINARAWELLIAMLAMEAAFGITGLIAAPIIYAYYKEEL
                     RAKALV"
     gene            6364..6840
                     /locus_tag="VEW08_12690"
     CDS             6364..6840
                     /locus_tag="VEW08_12690"
                     /inference="COORDINATES: protein motif:HMM:NF020480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity protein Tdi1 domain-containing
                     protein"
                     /protein_id="HYJ41641.1"
                     /translation="MAISGSDLTCTPNQDAIDRLAEAWAWLLKEPFTPVLFSALGDMF
                     FEPDSGGIWWLNTGTAELTQVADSVDDFHEKLGGDIATEWFMPGLVEQLHAAGKVPEP
                     GECYTYVTLPVFAEGKYEVDNLNPVPAGEHFSVTGHILHEIAELPDDAKAKLHTVQ"
     gene            complement(6853..7833)
                     /locus_tag="VEW08_12695"
     CDS             complement(6853..7833)
                     /locus_tag="VEW08_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ41642.1"
                     /translation="MDKRKLGNGLEVSALGLGCMGMNHGYGKPGDRQEMIQLLHAAVE
                     RGVTLFDTAEVYGPFINEELVGEGLAPFEGRVVIATKFGFRIGAQQSGVDSRPEHIRE
                     VAEASLKRLKIDTIDLFYQHRVDPDVPIEEVAGTVKDLIREGKVRHFGMSEAGAQTIR
                     RAHAVQPVTALQSEYSLWWREPEKEILPTLEELGIGFVPYSPLGRGFLTGKMTASATF
                     EKTDFRANLPRFQPAALAANQALVDLLAVIAARKQATPAQIALAWLMARKPWIVPIPG
                     TTKLARLEENIGAAALELTREDLRDIETAAAHVEVQGERYPESLERMTGR"
     gene            complement(7889..8977)
                     /locus_tag="VEW08_12700"
     CDS             complement(7889..8977)
                     /locus_tag="VEW08_12700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ41643.1"
                     /translation="MNTGISIRLRQISVVAFMLAASMHTLGASPDKAGPLVIQTQGSF
                     LVGGKVVTNPGKYDPYTRTADGQTFRGDHAYVFYQLPPKPRKYPLIMWHGFGQFSKTW
                     ESTPDGREGYQNIFLRRGYGVYVIDQPRRGNAGRSMVETKITPTPDEQTWFGTFRVGI
                     WPNYFPGVQFSRDPEALDQYFRSMTPNTGPFEMEVVTDAASQLLNKTGPAILVTHSQA
                     GGPGWVTAIKNPNVRGIVAYEPGSSFVFPESEVPAPMPSAGGTLEATGIPAADFKKLT
                     RIPIVIYYGDYIPEKPDANPGQDGWRVRLAMARLWRDAVNREGGDVTVVHLPEIGIRG
                     NTHFPFSDLNNLEIADLMSKFLSDKKLD"
     gene            complement(9048..9947)
                     /locus_tag="VEW08_12705"
     CDS             complement(9048..9947)
                     /locus_tag="VEW08_12705"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYJ41644.1"
                     /translation="MARENLNDIVAFLAVAREKSFTKAAAQLGVSQSALSHTIRGLES
                     RMGLRLLTRTTRSVSPTRAGERLVTAVGPHFEDIQMEIEALGELRDRPTGTVRITAGL
                     HATETILWPRLQPLLKKNPDVHLEITVDYGLTNIVADRYDAGVRLGEQVAKDMFAVRI
                     GPDFRMTVVAAPSYFANRPAPRTPQELTDHDCINLRLPTYGGLYVWEFERGDREINVR
                     VDGQLTFNSSRQILDATLAGFGVAYLPEDTVAPHLERGKLVSVLENWCAPFPGYHLYY
                     PTRRESSPALALFIDALRYKRNK"
     gene            10102..10605
                     /locus_tag="VEW08_12710"
     CDS             10102..10605
                     /locus_tag="VEW08_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ41645.1"
                     /translation="MKHLMPILLAACVTLAAAANSDKPQLTVMRAGARPVIEGPAANF
                     TGKATIDRQVRPDASKRIMGSLVSFEPGARTAWHTHPLGQTLIVTSGCGWVQSEGGKK
                     EEIRAGDVIWTPANVKHWHGAQPNAAMSHYAIVEPLNGNSAEWMEKVSDEQYASQEAS
                     TCKTAKT"
     gene            10584..11213
                     /gene="paoA"
                     /locus_tag="VEW08_12715"
     CDS             10584..11213
                     /gene="paoA"
                     /locus_tag="VEW08_12715"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF008514.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HYJ41646.1"
                     /translation="MQDSEDINRRELLIAGAATAAATAFPLTVNAQDSGTSAPVIAKV
                     GIEVNGKPHTLALDTRTTLLDALRDHLHLTGTKKGCDHGQCGACTVIADGRRINSCLT
                     LAVMHDGGKVTTIEGLGTPDKMHPMQTAFVKHDGFQCGYCTPGQICSAVAVLNEIKAG
                     VPSHVSADLLAAPQVTNAELRERMSGNICRCGAYSNIAEAIAEVAGSGA"
     gene            11210..12169
                     /locus_tag="VEW08_12720"
     CDS             11210..12169
                     /locus_tag="VEW08_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYJ41647.1"
                     /translation="MKSFSYERAKTPAEAAAAAARIKGARFIAGGTNLLDLMKLEVET
                     PAHLIDVNGLALDKIEVLPDGGLRVGALVRNTDLASDERVRREYPLLSRALLAGASGQ
                     LRNKATTAGNLLQRTRCAYFYDTNMACNKRNPGSGCSALGGVSRQHAVIGGSDACIAT
                     HPSDMAVAMRALDATVETVRPNGATRKIPIATFHRLPGDSPHIETNLQPGELITAVTV
                     PRPVGGTQLYYKVRDRASYAFALVSIALIQQKDGTGRVALGGVAHKPWRVESAERALP
                     SGSKATADGLLAGAKPTHDNEFKVKLVERTLAAVLAHAKELRA"
     gene            12166..14361
                     /gene="paoC"
                     /locus_tag="VEW08_12725"
     CDS             12166..14361
                     /gene="paoC"
                     /locus_tag="VEW08_12725"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF041671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HYJ41648.1"
                     /translation="MKFESAAGANPLDQGKVLGRATDRIEGKLKTTGTARYAYEWHEE
                     VPKAAYGYVIGSAIAKGRIVSIDVHGAKTAPGVIGVITAAEAGPLTKAALNTAHLLGG
                     PDIQHYHQAIAVVVAETFEQARTAAQRVQTKYAKAPGAFDLAAAKDGAAPAKIFGRPG
                     ESATGDFAGAFAAAPVQFDATYTTPDHAHVMMEPHASIAAWNGDKLTMWTSNQMIDWA
                     VRDMASTLGIARENIRIVSPYVGGGFGGKLFLRADALLAALAARATGRPVKIALQRPL
                     MINNTVHRPATIQRIRFGATPDGKLTALAHEGWAGDLPGGKAEASTRPGRLMYAAANR
                     MTALKLAVLDLPEGNAMRAPGDTPGQMALEVAIDEMAEKLKLDPIEFRILNDTQVDPE
                     SPSRPFSQRKLTECLRTGADKFGWQRRQAPGKVRENEWLIGMGVAGAIRDSPVVKSGA
                     RVKLDNRGMVIVESDMTDIGTGSYTIMGQTAAEMMGVSLDRVVVRLGDSAFPVASGSG
                     GQWGANCSTAGVYAACVKLRETVAQKLGVDPAMAEFIDGQVREGKRSVSLAQAARDGE
                     LAAEDAIEFGDLDDKNRLFSFGAHFVEVAVRAFTGETRVRRMLAVCSAGRILNPKSAR
                     SQIIGAMTMGVGAALMEELAVDKRHGFFVNHDLASYEVPVHGDIPHQEVIFLDEVDPL
                     SSPMKARGLGELGLCGVPAAIANAIYNATGIRVRDYPITLDKLIRGLPA"
     gene            complement(14358..15077)
                     /locus_tag="VEW08_12730"
     CDS             complement(14358..15077)
                     /locus_tag="VEW08_12730"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYJ41649.1"
                     /translation="MPINTTSILAANVAVMLGAILQASTGLGAGLIIVPLLALVSLAF
                     VPGPLVLASMALSGLMAYQGRRDIDGRGLPLLMVFLFVGVMLGALSISAIPVQRAGIA
                     FGILVLVAVVVSASMPRLERTIPVAVGAGVLSGFMGAISGIGAPILALIYQHEKPHKL
                     RATLGFIFFLSSIAILICLHFAGRFGWEETWLGLWLVPGYVLGFLVAPPIARLLDRNN
                     SRFAVLLISTLSAIVLIARSF"
     gene            15104..16633
                     /locus_tag="VEW08_12735"
     CDS             15104..16633
                     /locus_tag="VEW08_12735"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HYJ41650.1"
                     /translation="MEVFKWLLVLLVGAVALTAFARSLKLPYPSLLALGGAALALLSN
                     GSEFALHPDLALALFVAPVLLDAAFDTSLRDLKHYWVPVLSLVVVAVGITTFAVAWLV
                     HSLVPGMPWAAAIALGAIVAPPDAAAASAILRQLHLPHRMVVVLEGESLLNDATALLI
                     YRLAVSAAMGSTFTAGVIAWQAFAMLASVAVGFVLAHLYIRIVQRVTDVPSAIVMQFV
                     GAFGIWILADEVGLSAIVTIVAFAITAARITAELTPASLRIPSYAVWETAVFVLNVLA
                     FVIIGLQVRPVLDSLQDGERTQYLQIAGLVFATVVFVRIAWVFIYNRAAMLKAKLFGA
                     GSWPGDYISTVAGSTIVSWCGMRGIVTLATAFALPAVFPFRDLILLCAFCVVAGTLVV
                     QGLTLRPLIQLLGIKPDDTIAKEIRSAQAELARVAAAIIDEDASDAAQHLRAEFAMPS
                     DEQIADGSETEHAAHNRLRARIITAQRQALVRMRATGVISDDAFHPIEERLDRVEINV
                     R"
     gene            complement(16630..17079)
                     /locus_tag="VEW08_12740"
     CDS             complement(16630..17079)
                     /locus_tag="VEW08_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41651.1"
                     /translation="MRKRLVGGEILEVPVGNRFGYVQFLGEHREYGDAVLVSPTLHDR
                     QAHFPTGFFSKGYVTFYPAANSVTRKLVEVVAQSSPPSLPKRFRRPKSTLQDGAVESW
                     VIEGGWRNVVRQTLSDEERKLPIAEVSDHDFLRERIANGWTPEKDSR"
     gene            17278..>19165
                     /locus_tag="VEW08_12745"
     CDS             17278..>19165
                     /locus_tag="VEW08_12745"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HYJ41652.1"
                     /translation="MPSNATKAKEYGDAARKPVAGVHQEASPEITGSTLSEKNSSPKS
                     GSLDRVRANAAGQVTTNQGVPVADNQSSLKAGLRGPALLEDFILREKITHFDHERIPE
                     RIVHARGSAAHGYFECYAPQTKITRASVFADAGKRTPVFVRFSTVAGERGSADTVRDV
                     RGFAVKFYTDEGNWDLVGNNMPVFFIQDAMKFPDLIHAVKPEPHNAIPQAASAHDTFW
                     DFVSLMPESVHMQMWAMSDRAIPRSYRMMQGFGVHTFRMVNERGDSVFVKFHWNPVAG
                     THSLDWDEAVKLCGADPDFHRRDLWEAIEAGAYPEWELGLQVFTEQDAEKFSFDVLDA
                     TKIVPEELVPVKTVGKLVLNRNPDNFFAETEQVAFCTAHIVPGLDFSNDPLLAGRIHS
                     YVDTQISRLGGPNFHELPINAPLAPVANNQRDGMHRQALHRGRVAYEPNSLGGGCPFQ
                     AGAKGFTSFPQPVDEDKVRGKPEKFADHYTQAALFYDSQSPIEQAHIVRAFRFELTKV
                     QVPAIRERVVSQLVNVNSDLALQVATGLGIPVPKAQTPVRSRNMKPEVKTSPTLSLFA
                     RPGDGSIRTRNIALLVADGIDGASLDALHRKLSSEGAVPRYVGLRLGSVKTSDGQKLE
                     VDATL"
BASE COUNT         3617 a         6207 c         5806 g         3535 t
ORIGIN      
        1 gtggagatgg gtgaccgctc gcgggccagc cgtgcccgcg ccagcctcag gatgtcggcg
       61 taattcgccc gaaagcgctc ttcgaaccct tccgccaagc agcctccggc ccgaacaaga
      121 tcgggggcga ttctgcatga cggttctacg ggaaaccatg gccggacagg cttgctagcc
      181 ccgaagtgcg aagagttacc tgttaaagcg taatccccga ccaaagcagc tcacggtgcc
      241 gggtggggaa agtggggagt gcgtgttcgc gctcgggtta tcccgcctta cctcgccact
      301 ccccactttc cccacccggc accgtcaacc ccagctccac ctgcttcatc ttctcgatgg
      361 catcgcgcag gcgcgcggcc tcctcgaact ccaggttccg cgcgcgcttg tacatctccg
      421 cttccatgcg cttgatctct cgaacgatct gctccggcga cagcagccgc agattcggcg
      481 cggcctcttc cgccccccgc ttcttcttgc ccatcttgta gccgaagtcg gtggtatcgg
      541 cgcgcgcgcc ctccatcaca tccgtgacgt tcttgcgaat gctgaccggc gtgagtccgt
      601 gttcgaggtt gtacgccagc tgcacgttgc ggcggcggtc cgtctcgtcc atcgcgcgtt
      661 gcatcgaccc cgtgatgcgg tcggcataga gaatggcctt gccattcacg tggcgcgccg
      721 cgcggcccat ggtctggatc agcgagttgt cgctgcgcag gaacccttcc ttgtcggcat
      781 cgagaatggc cacgaggctc acctcgggca tgtcgagacc ctcacgcaac aggttgatgc
      841 cgacgagcac gtcgaacttg ccgagccgca gatcgcggat gatctccacg cgctccacgg
      901 tctccacgtc cgagtgcagg taccgcacct tcacgccgtg ctcgtgcagg tactcggtga
      961 gatcctccga catgcgcttg gtgagcgtcg tgatcaacac gcgctcatcc tgggcggcgc
     1021 gcagcttgat ctcggaaagc acgtcgtcca cctgggttgc gacaggccgc acttctacca
     1081 ccgggtccac caggtgcgtc gggcgcagca actgctccac ggtctgcgcg gcatggcgtg
     1141 cttcatactg acccggcgtt gccgatacaa agatcatctg cggcgacagt tgctcccact
     1201 cttcgaactt gagcgggcga ttgtcgagtg cggacggcag tctgaatccg tactccacca
     1261 gggtctcttt acgcgaccgg tcgcccttgt acatggcgcc caattgcgga atggtctgat
     1321 gcgattcgtc gacgatcagc agcgcgttct tcggcagata gtcgaacagg cacggtggtg
     1381 gttcgcccgg ttcccggccc gagagataac gcgaatagtt ttcgatgccc gagcagtacc
     1441 cgacctcttt catcatctcc atgtcgaaca tcgtgcgctg ctcgaggcgc tgcgcctcca
     1501 ccagcttgtt ctgttcgcgc agaaccttga gccggtcgcg cagttcctcg cggatgaaat
     1561 cgatggcgcc gatgaggcgt tccttcggcg tcacgtagtg cgatcccgga tacaccgtgt
     1621 agcgcggcaa cttgcgctgg atctcgccgg tcagcggatc gaacgtagcg atggactcga
     1681 tggtttcatc gaacagctcc acgcgcacgg cctcgcgcgc cgactcggcc ggaaagatgt
     1741 cgatgacatc gccgcgcaca cgataggtgc cggcctgcag gtccatctcg ttgcgcgtgt
     1801 attgcatgtc cgcgagacgg cgcagcagcc gtcgctggtc catgcgctcg ccgcggtcca
     1861 ggtgcaggat catggcgtgg tacgccgccg ggtcgcccag gccatagata gccgacacgg
     1921 tcgccacgat gatggcatcg ctacgctcga gcagcgcctt cgtggccgag aggcgcatct
     1981 gctcgatgtg ctcgttgatc gacgcgtcct tctctatata tgtgtcgctc gatggcacgt
     2041 aggcctcggg ctggtagtag tcgtagtacg aaacaaagta ttcgaccgtg ttctcgggga
     2101 agtactgctt gaactcgccg tacaattgag cagccagcgt cttgttgtgc gccatcacga
     2161 gggtgggacg ctggacctgc tggatgacgt tcccgatgct gtatgtgttg tggacgaaca
     2221 agccgccatg gccggcgaag aaggtttccg gccccggcac ggacaggtcg tagacgtacg
     2281 gatgcgaata ttcaatgcgg tcgagcttct tcactcgagt ccatcggacg cggcacagac
     2341 acgcgatctc gcgcatcttc gcccaccatt gcaagtccac cactttccgt tcgagcgcct
     2401 cacgagccag cccgcccagt acccggcgca acagacgtcg gcttggtttg cgtcggccat
     2461 tttcgagcat ggtgatcatg ggacgacaac aacccgcgcg tgccgccact tgggtttgat
     2521 tcaagccaag tgactggcgc aactcgcgca ggaccgacgg ggcgaccggc acgacgtcga
     2581 cgttcgtgtc cgtcttccgc gtgaagaatt tctgaagtcg agcagacttt tctgggtgtc
     2641 caaatccgat cgacttttcg aatcgctcga gatcgactcg tccggaaatc gcaatgtgcc
     2701 agtacaaatc acccttgtgg ttcgaattcg tcgcacgctt catcgcgcgc cgcaaccgcg
     2761 catgaatacc aaatcgcttc aacgcaaacg ccagatcaga tgccaactgc tcgcttgcgg
     2821 tcgtcgcgat gacttcgccg ccgtacccca ccgttccgtc accatcaaag tacgagcgca
     2881 ggagaatccc gagcgagcga tcggagagcc gcgtccagaa atccggcagg cgtttgtgcc
     2941 acgcgctggg tccgcaagtc ttcgaaagca gcttggtcag agccagcgat gaaatctgat
     3001 agtcggtgca gggacgtacg gagaacggaa tccccaattc attcaatgcc aactcgattc
     3061 tggagcgaat gaccggatga cggttggcaa tcgtgaagaa tcgtgcctgc gaattgccct
     3121 cggcgatgta gatgccaaac aacgatagaa gagaatccgt aagtggtaat tgagccggaa
     3181 ggcgacattc gcgcttctta ccaccgatag tcaccgagcc atcggcgtac gaggcgccca
     3241 agtgcgccct cagctggtga tattgatgcg ccttgatgcc ggagcctgag tcctggtttc
     3301 gaatgtcgcg caacttcgcc cacggattga ggcgaaaact cgagaagctg tccctaagtc
     3361 ccgcgtagcc accattggca gcgcacagat tgatcgactc ctccgcgaat accgaaagcg
     3421 atgtgtcgtc caaatacgaa attgcatcca gtactcgaag gtcctcacta ctggatatcg
     3481 cttcgggcgt gggtatttga tcggactcga ggacttcttc cgtgcgtatg agcgtcagct
     3541 ttccgccgcg aaggacccaa aggttgtggt ccccggtgag cgaaactgcg cgaccgcact
     3601 gggtggtcaa tcgaaacatc gcgtcaggtg ccttgtggcg cagtagcgca ctgactgccg
     3661 cgcgagacgc gattcccgtg gctgggtcga acgcgtgcgt aaagtacgcg ccctcggcgc
     3721 aggcgaatcg ctcggtttct gaatcgccat tgagaaacgg aactgcatcg atgagtgcat
     3781 cgatgaatgg gcccgctttg acgattttcg tgcgctcttt gccttcaacg cgctcgacca
     3841 agtacagcgg gtcctcatac ccgatgcttt ttcccgaacc cgtcaccccc agcagcgtct
     3901 ggtgcttgaa cccatccctc agcccctcca ccagcttcgc aatcgcctgc ggctggtcgc
     3961 ccgcgggctc gtagtccgct ttcagtacaa aaggcttgtt ttccatgact ttagacgctc
     4021 agtgagccgt ggcgaattga accggatcgc gtaatatacg cgcccgtttg accatcccaa
     4081 ccccccactt caatcaaggg tcctaaaacc gtgcccaatc agctctcgcg acgcgttcaa
     4141 cgcgtcaagc cttcgcccac tctcgcagtc accgcgcgcg ccgcgaaact gaaagccgag
     4201 ggcaaggaca tcatcggcct gggggccggc gagccggact tcgacacgcc cgcgcacatt
     4261 gccgaagccg gcatcgacgc gatccgcaag ggcttcacgc ggtacacgaa cgtcgacggc
     4321 atcgtcgagc tcaaggacgc catcatcgcg aagttcaagc gcgacaatca gatcagctac
     4381 gagcgcaacc agattctcgt cagctcgggc gccaagcaga ctatctacaa cctctgcatg
     4441 gccgtgctcg acccgggtga tgaagccgtc attcccgcgc cgtactgggt gagctacccc
     4501 gacatggtgt tgctggccga cggcctgccg gtcacgccca tctgccccgc ttcgcagggc
     4561 tacaagctca cgccgcggca gctcgaagcg gccatcacgc cgaagacgcg tttgctgttg
     4621 atcaattctc catgcaaccc gaccggcgct gcctacacgc gtgccgagtg gcgcgcgctg
     4681 gccgacgtgc tcgtgcagca cccgcgcgtc atcgtgggca ccgacgacat gtacgaaaag
     4741 atctggtggg gcccggagcc cttctccagc ctgctcaccg tcgcgccgga actgtacgac
     4801 cgcgtggtca ccatcaacgg cgtgtcgaaa tcgtatgcga tgaccggctg gcgcctcggc
     4861 tactgcggcg gccccaagga aatcgtgaac gccatgtcca cgatccaggg acagagcacc
     4921 agcaacgcgt ccagcatcac gcagaaggcg gcgctcgcgg cgctcaacgg cgaccagaag
     4981 cctgtcgaac tcatgaacat cgagttcaag aagcgtcacg actggttcat cggcgcgctc
     5041 aaccgcctgc cgggcatcgt cgcattgccc tgcgccggcg cgttctacgc ctgggcagat
     5101 tgcagcgccg ccatgatggc cttcggcatc agggacgata atgcgttcgc cgaaatgctc
     5161 atcaacgacg cgggtgttgc cgtggtgccg ggctcgggct tcggcgcgcc gggtcacttc
     5221 cgcgcgtcgt tcgccaccag catggccgtg ctcgaacaga gcatcgagcg catgaagaag
     5281 gtgctcgaag ccggaccggt gaagaagtcc gcctgaacca cgggagccgc ggcaaatcat
     5341 gatccccgcg gctcccactc ccatcacgcg cgccgactac acggcgtgga tcctcgtcgc
     5401 gctggcgctc ggcctcgtca tcgagcttca cctgctgtcg gggctgctcg caggcctgct
     5461 cgttttcaag ctggtcgatg tcctcacacc gtggctgagg ctgcgcgcgc tcggccgcga
     5521 tggtccacgc gtgctcgccg tgacgctgat cgcaaccgcg gtcatcgccg cgctcatcgg
     5581 cattggcatc ggcctcgcga ccctgatgcg caattccggt gaaagtgtcc cgctgctcat
     5641 ggagaagatg gcgcagatca tcgaggatgc ccgcgcgatt ctcccggcct cactccaaaa
     5701 atacgtaccc gaagattcgg caaccctgcg gcgcacgctc gccgaatggc tgcgatcgaa
     5761 tgccggagcg ctgcagctcg ccggccgtgg catcggccgc tcagtcgtcc acgtgctcat
     5821 gggcatgatc atcggcgcgc tgctttccat gcagaaggcc gcacatccgc acgaccgccg
     5881 cccactcacc gaacacatcg tgcggcatgc cgcccggttc gccaccgcgt tccagcgcgt
     5941 ggtgttcgct cagttctgga tctcgctgat caacaccttc ttcacgtggc tgtacctcga
     6001 cgtggtgttg cgcctgttcg gcatcgatct gccgctggtg aaaatcctgg tggcgctcac
     6061 gttcatcgtc ggcctgctgc ccatcatcgg caacctgatc tcgaataccg ccatcgtcgt
     6121 cgtgtgcctg agccagggtg tgccggtggc cgccgcatcg ctggtctacc tcgtcgtgat
     6181 ccacaagctg gaatatttcc tcaacgcgcg catcattggc tcgcacatca acgcccgcgc
     6241 ctgggagctg ctgatcgcga tgctagccat ggaagccgct ttcggaatca ccggattgat
     6301 cgccgcgccg atcatctatg cctattacaa agaagagttg cgcgcgaaag cgctggtctg
     6361 accatggcga tcagtgggtc tgatctgaca tgcacaccta accaggacgc catcgatcgt
     6421 ctcgccgaag cgtgggcatg gctgctgaag gaaccgttca ctcccgtgtt gttctcggcg
     6481 ctcggcgaca tgttcttcga accggactca ggcggcatct ggtggctgaa caccggcacc
     6541 gccgagctca cgcaggtcgc ggattccgtc gacgattttc acgagaagct gggcggcgac
     6601 atcgccaccg agtggttcat gcccgggctc gtggagcaac tgcacgcggc agggaaagtc
     6661 cctgaacccg gcgagtgcta cacgtacgta acgctgccgg tattcgccga aggcaaatac
     6721 gaagtcgaca acctgaatcc cgtgccggct ggtgaacact tctccgtgac cggccacatc
     6781 ctccacgaga ttgcggagct gcctgacgac gccaaggcca agctgcacac cgtgcaatga
     6841 gatcgacgga gctcaacgcc ccgtcatgcg ttccagcgac tccgggtaac gctcgccctg
     6901 cacctcgacg tgcgcagcgg cagtttcaat atcgcgcaga tcttcacgcg tgagctcgag
     6961 cgcggccgca ccgatatttt cttcgagccg cgcgagcttg gtcgttccgg ggattggcac
     7021 gatccacggc ttccgcgcca tgagccaggc gagcgcgatc tgcgccggcg tcgcctgttt
     7081 gcgcgcggcg atgacggcta acaagtccac cagcgcctgg ttggccgcga gcgccgcggg
     7141 ctgaaagcgc ggcaggtttg cgcggaagtc ggtcttttcg aaggtggcac tggcagtcat
     7201 cttgccggtg aggaatccgc ggccgagcgg actgtagggc acgaagccga tgccgagttc
     7261 ttcgagcgtg ggcagaattt ccttttccgg ctcgcgccac cacagtgaat actcactctg
     7321 cagcgcggtg accggttgca cggcatgcgc tcgccgaatg gtctgcgcgc ccgcttccga
     7381 catcccgaag tgtcgcacct tgccctcgcg aatcagatcc ttcaccgtgc ccgccacttc
     7441 ttcgatcggc acgtcgggat ccacacggtg ctggtagaac agatcgatgg tgtcgatctt
     7501 cagtcgtttg agcgaagcct cggcgacttc gcggatgtgt tcggggcgac tatctacgcc
     7561 cgactgctga gcgccgatcc ggaagccaaa cttggtggcg atgaccacgc gcccctcgaa
     7621 cggcgccagc ccctcgccca ccagctcctc gttgatgaac ggtccataca cctcggcggt
     7681 gtcgaacaac gtgacgccac gttccacggc cgcgtgcaac agctggatca tctcctgccg
     7741 gtcaccgggc tttccgtaac cgtgattcat gcccatgcag ccaaggccga gcgccgacac
     7801 ttcgagtccg tttccgagtt tccgcttgtc catgatggaa gtaagcccgg gacccgggcc
     7861 gcgcgcaagt ctcagaattg cgacgctttc aatccagttt cttgtcggag aggaacttcg
     7921 acatgagatc ggcgatttcc agattgttca ggtcggagaa tggaaagtgg gtgttgccac
     7981 gtatgccaat ctccggaaga tgcacgacgg tgacatcacc accctcccgg ttcactgcgt
     8041 cgcgccacaa gcgcgccatc gcgaggcgaa cgcgccagcc gtcctgaccc ggattggcat
     8101 ccggcttttc cggaatgtag tcgccgtaat agatgacgat cggaatccgt gtcagcttct
     8161 tgaagtcggc cgccggaatt cccgtcgcct ccagcgtccc gccggcgctc ggcatcggcg
     8221 ctggcacctc actctcgggg aacacaaaac tgcttccagg ttcgtacgct acgatacctc
     8281 tgacattcgg gttcttgatg gcggtgaccc accccggtcc tccggcttgt gaatgcgtta
     8341 cgagaatggc cggaccggtc ttgttcagga gttgtgaggc ggcgtcggtc acgacctcca
     8401 tttcgaatgg acccgtgttc ggcgtcatcg acctgaagta ctggtcgagt gcctccggat
     8461 ccctggaaaa ctgcacgccc ggaaagtagt ttggccagat acccacgcga aacgtgccga
     8521 accaggtctg ctcgtcgggc gtcggcgtga tcttcgtctc gaccatgctg cggccggcat
     8581 tgccgcgccg cggctgatcg atgacataaa cgccgtagcc gcgacgcaag aaaatgttct
     8641 ggtaaccctc gcgcccgtcg ggtgtggatt cccaggtttt agagaactgg ccgaaaccat
     8701 gccacatgat caacggatat ttccgcggct tcggcggcaa ctggtagaac acgtaagcgt
     8761 ggtcgccgcg gaatgtctgg ccgtcggcag tgcgtgtgta aggatcgtac ttgccggggt
     8821 tggtgacgac cttgccaccg acgaggaagc tgccctgcgt ctgaatgacg agcggacccg
     8881 ccttgtcagg tgaagcgccg agcgtgtgca tcgaggcagc cagcatgaat gcgactaccg
     8941 agatttgacg cagtctgatg gaaattcctg tgttcattcg atgcccagtt taggcgacgg
     9001 gaggcggccg attaaggcgc gtcagcgaat gcacccatga gccaggctca tttattgcgt
     9061 ttgtagcgca gcgcatcgat gaacagcgcg agcgcgggag aagactcacg ccgcgtcggg
     9121 taatagagat ggtagccggg aaacggcgcg caccagtttt cgagcacgga gacgagtttt
     9181 cccctctcga gatgcggcgc gaccgtatct tccggaaggt atgccacgcc aaaaccggcg
     9241 agcgtggcat cgaggatctg ccggctggaa ttgaacgtga gctggccatc cactcgcaca
     9301 ttgatttccc ggtctccccg ctcgaattcc cagacataga ggccaccata cgtgggcagg
     9361 cgcaggttga tgcagtcgtg gtcggtaagc tcctgcggcg tgcggggcgc gggcctgttt
     9421 gcaaaatagg acggcgccgc caccaccgtc atgcggaagt caggcccgat gcgcacggca
     9481 aacatgtcct tggccacctg ctcaccgagg cgcacgccgg catcgtatcg gtccgccacg
     9541 atgttcgtca gcccgtaatc cacggtgatc tcgagatgaa cgtcgggatt cttcttcagc
     9601 agaggttgca gcctcggcca cagaatcgtt tccgttgcgt gcaggcccgc ggtgatgcgc
     9661 accgtcccgg tgggtctatc gcgaagttcg ccgagcgcct cgatctccat ctggatgtct
     9721 tcgaagtgcg ggcctacggc ggtcacgagt cgttcgcccg cgcgggtcgg tgaaacgctt
     9781 cgagtggtac gcgtgagcaa tcgcaagccc atgcgcgact ccaggccgcg aatcgtatgg
     9841 ctcaatgcgg attgggacac accgagctgg gcagccgcct tggtgaagct cttttcacga
     9901 gcgaccgcca ggaaggccac gatgtcgttc agattctccc gcgccattga tgtggttcct
     9961 ttcgtgaccc agctgcacgt aggactcgtt taacggcccc cgcagcattg atgaatttac
    10021 ctcacgagta cgtgcaggtt tagccatctt atcggcggcc gccgcgcttg gtaacttggc
    10081 tcagtcgtcg aaggagctcg aatgaaacac ctcatgccaa tcctgctggc ggcctgcgtg
    10141 acgctcgcgg ccgcagcgaa cagcgacaag ccgcagctca ccgtcatgcg cgccggcgca
    10201 cgtccggtca tcgagggacc cgcggccaac ttcaccggca aggcgaccat cgaccggcag
    10261 gtccgccccg acgcttcgaa acgcatcatg ggctcgctcg tttcattcga gcccggcgcg
    10321 cgaactgcgt ggcacacgca cccgctcggc cagacgctga tcgtcacctc gggctgcgga
    10381 tgggtgcaaa gtgagggtgg aaagaaagaa gagatccgcg ccggcgacgt gatctggacg
    10441 cccgcgaacg tcaagcactg gcatggtgcc cagccgaatg cggcgatgtc ccactacgcc
    10501 attgtcgaac cgctgaatgg caacagcgcg gaatggatgg aaaaggtcag cgacgagcaa
    10561 tacgccagcc aggaagccag cacatgcaag acagcgaaga cataaaccgg cgtgaacttc
    10621 tgatcgccgg cgcggccacc gcggccgcca ctgcatttcc gctgacggtc aacgcgcagg
    10681 attcaggcac ctcggctccg gtgatcgcga aggtgggaat cgaggtgaac ggtaaaccac
    10741 acaccctcgc gctcgatacg cgcaccaccc tgctcgacgc gctgcgcgat cacctgcatc
    10801 tcaccggcac caagaagggt tgcgatcacg gtcaatgcgg cgcgtgcacc gtcattgctg
    10861 atggccgccg catcaactcc tgtctcacgc tcgccgtgat gcacgatggc ggcaaggtca
    10921 cgaccatcga gggtctcggg actcccgaca agatgcatcc gatgcagacc gcgttcgtca
    10981 aacacgacgg gttccagtgt ggctactgca cgcccgggca gatctgctcg gctgtcgccg
    11041 tgttgaacga gatcaaggcc ggcgtgccga gccacgtgag cgcggacctg ctcgcggcgc
    11101 cgcaagtcac caacgctgaa ttgcgcgaac gcatgagcgg caacatctgc cgctgcggcg
    11161 cgtactccaa tatcgccgag gccatcgccg aagtcgcggg gagtggtgca tgaaatcttt
    11221 cagctacgaa cgcgcgaaaa ctcctgccga ggcggctgcg gctgccgccc ggatcaaggg
    11281 cgcgcgattc atcgccggcg gcaccaacct gctcgatctc atgaagctcg aggtcgagac
    11341 gccggcacat ctcatcgatg tgaacggact cgcgctcgac aagatcgagg tcttgcccga
    11401 tggcggcctg cgcgtcggcg cgcttgtgcg caacaccgat ctcgcctccg acgagcgcgt
    11461 tcgccgcgaa tacccattgc tgtcgcgcgc gctgctcgcg ggcgcttcgg ggcagctgcg
    11521 taacaaggcg acgacggcag gcaatctgct ccagcgcacg cgctgcgcgt acttctacga
    11581 caccaacatg gcctgcaaca agcgcaaccc gggaagcggt tgcagcgccc tcggcggcgt
    11641 cagccggcag catgcggtga tcggcggcag cgacgcgtgc atcgccacgc atcccagcga
    11701 catggcggtg gccatgcgtg cgctggatgc caccgttgag acggtgcggc ccaatggcgc
    11761 cacacgcaag attccaatcg caacgtttca ccggcttccc ggtgactcgc cacacatcga
    11821 aacgaatctg caacccgggg aattgatcac cgcggtcacg gtgcccagac ccgtgggcgg
    11881 cacgcaactc tattacaagg tgcgtgatcg agcctcgtat gcattcgcac tggtgtcgat
    11941 cgctttgatc cagcagaaag acggcactgg tcgcgtggcg ctcggcggcg tcgctcacaa
    12001 accctggcgg gtggagagtg ccgagcgcgc cctgccgagc ggttcgaaag ccactgcgga
    12061 cggcttgctc gcgggcgcca agcccacgca cgacaacgag ttcaaggtga agctcgtcga
    12121 gcgaacgctg gccgccgtcc tggcgcacgc gaaggagctg cgcgcatgaa attcgaaagc
    12181 gccgccggcg ccaatccgct agatcaggga aaggtgctcg gacgtgccac cgaccgcatc
    12241 gaaggcaaac tcaagactac gggcaccgcg cgttacgcct acgaatggca tgaagaagtc
    12301 ccgaaggcgg cgtacggcta cgtcatcggg tcggccatcg ccaagggccg catcgtttcg
    12361 atcgacgtgc acggcgcgaa aactgcgccg ggcgtgatcg gtgtgatcac cgccgccgag
    12421 gcggggccgc tgacgaaggc cgcgctcaac accgcccatc tactcggcgg gcccgacatc
    12481 cagcattacc accaggcgat cgccgtggtg gtggccgaga cgttcgagca ggcgcggacc
    12541 gcggctcaac gtgtgcaaac gaaatatgca aaggcacctg gcgcattcga tctcgcggct
    12601 gccaaggatg gcgctgcgcc cgcgaaaatc tttggtcgtc caggtgagtc cgcgacaggc
    12661 gacttcgccg gcgcattcgc cgcggctccc gtgcagttcg acgccaccta cacgacaccc
    12721 gaccacgcgc acgtaatgat ggagcctcac gcttcgatcg ccgcctggaa tggcgacaag
    12781 ctcaccatgt ggacctcgaa ccaaatgatc gattgggcgg tccgcgacat ggcaagcacc
    12841 ttgggcatcg ccagggagaa tattcgaatc gtctcgccgt atgtgggcgg cggttttggc
    12901 gggaaacttt ttctgcgcgc agatgctctg ctcgcggcgc tcgccgctcg cgcgacgggt
    12961 cgtcccgtga aaatcgcgct gcagcggcct ctcatgatca acaacaccgt gcaccggccg
    13021 gccacgattc agcgcatccg cttcggcgcg actcccgacg gaaaactcac cgcgctggcg
    13081 cacgaaggct gggcgggcga cctgcccggc ggcaaggcgg aagcaagcac gcggccggga
    13141 cgcctgatgt atgccgccgc gaatcgcatg accgcgctca agcttgcggt gctcgacctg
    13201 cccgagggca atgcaatgcg cgcgcccgga gatacgcctg gccagatggc gctcgaggtc
    13261 gccatcgacg aaatggccga gaagctcaag ctcgacccca ttgaattccg gatcctcaac
    13321 gacacgcagg tcgatcctga aagtccgtcg cgtccgttct cgcaacgcaa gctcaccgag
    13381 tgcctgcgca ccggtgccga caagttcggc tggcaacgcc ggcaggcgcc aggcaaggtc
    13441 cgcgagaatg aatggctgat cggcatgggt gtggccggcg ccattcgcga cagccccgtc
    13501 gtgaagtccg gcgcgcgagt gaagctggat aaccgcggta tggtcatcgt cgaatccgac
    13561 atgacggaca tcggcaccgg cagctacacc atcatgggcc agacggcggc ggagatgatg
    13621 ggtgtgtcat tggatcgggt cgtcgtcaga ctcggtgact cggcatttcc cgtcgcgtcg
    13681 ggttccggtg gtcaatgggg ggcgaactgc tcgacagcgg gcgtttacgc cgcctgcgtg
    13741 aagctgcgcg agaccgtggc gcagaagtta ggtgtcgatc ccgcaatggc tgagttcatc
    13801 gatggtcagg ttcgcgaggg gaagcgttcc gtgtcattgg cgcaggctgc tcgtgatggc
    13861 gagctcgcgg ccgaggatgc aatcgagttc ggcgacctag acgacaagaa tcgcctcttc
    13921 agttttggcg cccatttcgt ggaagtggcc gtacgcgcat tcaccggtga gacgcgcgtg
    13981 cggcgcatgc tggcggtttg ctccgcgggc agaatcctca acccaaaatc ggcccgcagc
    14041 cagatcatcg gcgccatgac catgggcgtg ggcgccgccc tcatggaaga actcgctgtc
    14101 gataagcggc acgggttttt cgtgaaccac gatctcgcca gctacgaagt gcccgtgcat
    14161 ggcgacattc ctcaccagga agtcatcttt ctcgatgagg tcgatccgct ttcctcgccc
    14221 atgaaggcac gcggtctcgg cgaactcggt ctctgcggtg ttcccgccgc catcgccaat
    14281 gccatctaca acgcaaccgg cattcgggtg cgcgattatc cgatcacact cgacaaactg
    14341 atacgcggcc tgcccgccta gaaactgcgg gcgatcagca cgatcgcgct cagcgtcgag
    14401 atgagcagca cggcgaagcg cgagttgttg cgatcgagca ggcgcgcaat cggcggcgca
    14461 accagaaagc cgagcacgta tcccggcacc agccacagcc cgagccaggt ctcctcccat
    14521 ccgaatcgcc ccgcgaaatg caggcagatg agaatggcga tggacgacag gaagaagatg
    14581 aagcccagcg tcgcgcgcaa cttgtgcggc ttctcgtgct ggtagatcaa cgcgagaatc
    14641 ggtgcgccga tgccggagat cgcgcccatg aatcccgaca gcacaccggc ccccacggcc
    14701 accggaatag tccgctcgag gcgcggcatc gacgcgctca ccaccaccgc gacgagcacc
    14761 aatattccga acgcaatgcc ggcgcgttgc accgggatgg cggagatact gagtgcgcca
    14821 agcatcacgc cgacgaacag aaacaccatc agcaaaggta aaccacgacc atctatatcg
    14881 cggcggccct ggtatgccat gagccccgac agcgccatgc tggcgagcac cagtggtccc
    14941 ggaacgaatg cgaggctaac cagcgcgagc aacggaacga tgatgagccc ggcgccgagg
    15001 ccggtcgaag cttgaaggat ggcgccgagc atgacggcaa cgttggcggc gagaatgctg
    15061 gtcgtgttga taggcatgcc ctacgttacc gtagaatccg cgcatggaag tgttcaagtg
    15121 gctgctggtc ctgctggtgg gcgccgtcgc gctcaccgcg ttcgcgcgct cgctcaaact
    15181 tccctacccc tcgctgctcg cgctgggtgg cgccgcgctc gcgctgctgt cgaatggctc
    15241 ggagttcgca cttcatccgg acctcgcact cgcgctgttc gtggcgccgg tattgctcga
    15301 cgcggctttc gacacttcgc tacgcgacct caagcactac tgggtcccgg tgctgagcct
    15361 cgtcgtggtc gccgttggca tcaccacgtt tgccgtagcg tggctcgtgc actcgctggt
    15421 gccgggcatg ccctgggccg cggccatcgc gctcggcgcc atcgtcgcac cgccggatgc
    15481 ggccgcggca tcggccatcc ttcgtcagct tcacctgccg catcgcatgg tggtcgttct
    15541 cgaaggcgag agcctgctca acgacgccac cgcccttctc atctatcgcc tcgcggtgag
    15601 cgcggccatg ggcagtacgt tcactgctgg cgtgattgcg tggcaggcgt tcgcaatgct
    15661 cgccagcgtc gctgtgggtt tcgtgctggc acatctatat atccgcatcg tgcagcgagt
    15721 gaccgacgtc ccgagcgcca tcgtcatgca attcgtcggc gccttcggca tctggatact
    15781 ggccgacgag gtcggtctct cggccatcgt gaccatcgtg gctttcgcca ttactgccgc
    15841 gcgcatcacg gctgaactca cgcccgccag cctgcgaatt ccttcctacg ccgtgtggga
    15901 aaccgccgtg ttcgtgctga acgtgctcgc cttcgtgatc atcggcctgc aagtgcggcc
    15961 ggtcctggat tcgctgcagg acggcgagcg cacgcaatat ctgcagattg ccggcctcgt
    16021 gttcgccacg gtcgtgttcg tgcgcattgc ctgggtgttc atctacaacc gtgccgccat
    16081 gctcaaggcc aaactattcg gcgcgggcag ctggccgggc gactacattt ccacggtggc
    16141 cggcagcacc atcgtcagct ggtgtggaat gcgcggcatc gtcacgctgg ccacggcctt
    16201 cgcgcttcca gccgtgttcc cgtttcgcga cctcatcctg ctgtgcgcgt tctgcgtggt
    16261 cgccggcacg ctggttgtcc aggggctgac gctgcgaccg ttgatccagc tgctcggcat
    16321 caaacctgac gacaccatcg cgaaagaaat acgcagcgcg caggccgaac tcgcgcgcgt
    16381 ggccgccgcc atcatcgatg aagacgcaag cgacgctgcg caacatctgc gcgcggagtt
    16441 cgcgatgccg agtgatgaac agattgccga cgggtcggag accgagcacg cggctcacaa
    16501 ccggttgcgc gcacgcatca tcaccgctca acggcaagcc ctggtgcgca tgcgcgctac
    16561 tggcgtgatt tcagacgacg cgttccatcc cattgaggaa cggctcgacc gcgtcgagat
    16621 caacgtccgc tagcggctgt ctttctcggg cgtccagccg ttcgcgattc gttcgcgcag
    16681 gaaatcgtga tcggagactt ccgcgatggg cagctttcgc tcttcgtcgc tgagtgtctg
    16741 ccgcacgaca ttgcgccagc cgccttcgat gacccacgat tcgaccgcgc cgtcctgcag
    16801 cgtcgacttc ggacggcgaa agcgcttggg aaggctcggc ggggatgact gcgcgacgac
    16861 ttcgaccagc tttcgggtga ccgagttggc cgccggataa aacgtgacgt atcccttgga
    16921 gaagaatccg gtcgggaaat gcgcctgccg atcatgcaac gtcgggctca cgaggacggc
    16981 atcaccgtat tcacgatgct cgccaaggaa ctgcacgtag ccgaatcgat tccccacggg
    17041 cacttcgagg atctcaccgc caaccaatcg ttttcgcatg taagccatgg gggtgattct
    17101 gtcacgtctt gcgggcgggc attgacggcg atttaccccg gattccttcc gctgcgccta
    17161 catcacgctt ttcgccaaaa aggcgctaat ggcggcatga aatcacgcaa ttcaaagccg
    17221 acacccttgc cccgcagatc gcgaaaagcc gcggccacag aggcgcttgc gcaatccatg
    17281 ccctcgaatg ccactaaggc gaaagagtat ggcgatgcag cgcgaaagcc tgttgccggt
    17341 gttcaccagg aagcatcacc agaaatcacc ggcagcacgc tcagcgagaa gaactcctca
    17401 ccgaagtccg gcagccttga ccgcgtgcgc gcaaatgccg cgggccaggt gaccaccaac
    17461 cagggcgtgc cggttgccga caaccagagt tcgctgaaag ccgggcttcg cggccccgcc
    17521 ctgctcgaag acttcatcct gcgcgagaag atcacgcatt tcgatcacga gcgcataccc
    17581 gaacgcatcg tgcatgcgcg cggctcagcg gcacacggct atttcgagtg ttacgcgcca
    17641 cagaccaaaa tcacgcgcgc ctcagtcttc gccgacgccg gcaaacgaac tccggtgttc
    17701 gtgcgcttct ccaccgtcgc cggcgagcgc ggctctgccg acaccgtgcg tgacgtgcgc
    17761 ggctttgcgg tcaagttcta taccgacgaa ggcaattggg atctcgtcgg caacaacatg
    17821 ccagtcttct tcatccagga cgccatgaag tttccggacc tcatccacgc ggtgaagccc
    17881 gagccgcaca acgccattcc gcaggccgcg agtgcccacg acaccttctg ggatttcgtg
    17941 tcgctgatgc ccgagtccgt gcacatgcag atgtgggcga tgtcggaccg cgccatcccg
    18001 cgcagctacc gcatgatgca ggggtttggc gtacacacct tccgcatggt caatgaacgc
    18061 ggcgacagcg tgttcgtgaa gttccactgg aatccggtcg ccggcacgca ttcgctcgac
    18121 tgggacgaag ccgtgaaact ttgcggagcg gatccggatt tccatcgccg cgatttgtgg
    18181 gaagccatcg aagccggcgc ctatccggaa tgggaactcg gtctgcaggt cttcaccgag
    18241 caggacgcgg agaagttcag cttcgacgtg ctcgacgcca ccaagatcgt gcctgaggag
    18301 ttggtgccgg tgaagacggt ggggaagctg gtgctcaatc ggaacccgga caatttcttc
    18361 gcggaaaccg agcaggtcgc cttctgtacg gcgcacatcg tcccgggtct cgacttcagc
    18421 aacgatccgc tgctggcggg ccgcattcac tcatacgtgg acacacagat ctcgcgcctc
    18481 ggcggcccta acttccacga gctgcccatc aacgccccgc tcgcgccggt tgccaacaac
    18541 cagcgcgacg ggatgcatcg ccaggcgctg caccgcgggc gcgtggcgta cgaacccaac
    18601 tccctgggcg gcggctgccc gttccaggcg ggcgcgaaag gcttcacctc gtttccacaa
    18661 cccgtcgatg aagacaaggt tcgcggtaaa ccggagaaat tcgccgatca ctacacgcag
    18721 gccgcgttgt tctacgacag ccagtcgccc atcgagcagg cgcacatcgt gcgcgctttc
    18781 cggttcgagc tcaccaaggt gcaggtgccg gccatccgtg aacgcgtcgt ctcgcaactc
    18841 gtgaacgtga attcagacct tgccctgcag gtggccaccg gtcttggaat ccccgtgccg
    18901 aaggcgcaaa cacccgtgcg ctcccgcaac atgaaacccg aagtgaaaac ctcgcccacg
    18961 ctgtcgctgt tcgcgcggcc cggcgatggc agcattcgca cgcgaaacat tgccctgctc
    19021 gttgccgacg gcatcgacgg cgcgtcgctc gacgcgctgc atcgcaagct ttcgtccgaa
    19081 ggcgccgttc cgcgctacgt gggattgcga ctcggctccg tcaaaacctc agatggccag
    19141 aagctagagg tggacgcgac gctcg
//
LOCUS       DASQJS010000316         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2700742, whole genome shotgun sequence.
ACCESSION   DASQJS010000316 DASQJS010000000
VERSION     DASQJS010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2700742"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VEW08_12750"
     CDS             <1..301
                     /locus_tag="VEW08_12750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ41653.1"
                     /translation="PESSEDGDESYSPAAYLPAPDADPAAQIENAEWEDTTGDRLAQA
                     MKTLDPRAQDIVVSRWTGEGKVTLHDLAEKYGVSAERVRQIEANAIKKLRGLMTA"
     gene            415..1242
                     /locus_tag="VEW08_12755"
     CDS             415..1242
                     /locus_tag="VEW08_12755"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ41654.1"
                     /translation="MTKSALNPQGEQMADESMVRTLAAQIEAIWPQEKTFINFYRLPR
                     NARVLDVGAGTGEFSAKIAELLPEAQVLGVELLAASVEHASDRHAALAPRLKFETGDA
                     FHLAQPDNTFDLVANRHMIQSVPHVDRILAELVRVTRPGGRVHVLAEDYSMLHMMSGP
                     LDPDVFWRKGPVEFSMRTDTDSRIGRRAWTLMREAGLIDVRVDYVVVDTLRVPRRVFA
                     DIITAWRDGYVNALATPEFPAATVRQHFNEIIASILNPDHYAVWHIPIVSGMKPRAP"
     gene            complement(1295..1972)
                     /locus_tag="VEW08_12760"
     CDS             complement(1295..1972)
                     /locus_tag="VEW08_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYJ41655.1"
                     /translation="MNKIFVRTTLLAIAAAFTVAGCQTTDAYTGEKKVNNASKGAGIG
                     AIAGAVIGAATGDNSKERRERALIGAGVGALSGAGIGAYMDKQEAKLRAQLQGTGVSV
                     TRNGNDLILNMPGNITFKTASSDLNAGFYKVLDSVTIVLKEFDKTLIDVEGHTDNVGD
                     DSYNQNLSVQRAGSVGSYLQSHGVNSQRIVTVGAGESRPVASNDSAEGRQQNRRVELK
                     LQPITAG"
     gene            complement(2065..2610)
                     /locus_tag="VEW08_12765"
     CDS             complement(2065..2610)
                     /locus_tag="VEW08_12765"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HYJ41656.1"
                     /translation="MSNKEEDMPLMQRLMKFVARSRILPDARLLELYPPFVPMRIKVL
                     EISENWRTVRILLPLNARSRNPGGVMFGGYQAALADPIAALACARVFPGYSVWTRAMS
                     ISFDHGGTSDLEMRFALTPAQEQDIRIGLQNEGRATPTFEYGYVRNDGVCCTVIRNTV
                     AIRPRGYKRATTPPASPENVE"
     gene            2658..3125
                     /locus_tag="VEW08_12770"
     CDS             2658..3125
                     /locus_tag="VEW08_12770"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HYJ41657.1"
                     /translation="MRTVEQWFGEYGESHSNPRNELLHIICVPPIVLTVIGFIWAIPV
                     PAAFADVSPWLNWATITIALAVVYYFTLSVKLGIGAAIALGILALIVSWLDTLAWPLW
                     LTCLVIFAIGWVGQFIGHAIEGKRPSFFKDLQFLLIGPLWLLGHLYRRLGINY"
BASE COUNT          589 a         1023 c         1006 g          636 t
ORIGIN      
        1 gcccgagtcg agcgaggatg gcgacgagtc ctattcgccg gccgcctatc taccggctcc
       61 ggatgccgac cccgcggcgc agatcgagaa tgccgagtgg gaagacacca cgggcgatcg
      121 cctggctcag gccatgaaga cgctggatcc gcgcgcgcag gacatcgtgg tcagccgctg
      181 gaccggtgag ggcaaggtca cgttgcacga cctcgcagag aagtacggcg tgtcggcgga
      241 acgcgtgcgc cagatcgagg ccaatgcgat caagaaactg cgaggcctga tgacggcctg
      301 aagcgcctgt tcagactcaa tattgaagcc ccggccataa accggggctt ttctttttca
      361 atggtaagtt tttgtatagt tgcgacccac gggtccgtgg attaatccac caccatgacg
      421 aaatcagcac tcaatcccca gggcgagcag atggcggacg aatccatggt ccgcacgctg
      481 gccgcgcaga tcgaagcgat ctggccgcag gaaaaaacgt tcatcaactt ctaccggctg
      541 ccgcgcaacg cgcgcgtgct cgacgtcggc gcgggcacgg gtgagttcag cgcaaaaatt
      601 gccgagctgc tgcccgaggc gcaggttctg ggcgtggaac tgctcgcggc ttcggtcgaa
      661 cacgccagcg atcgccacgc ggccctcgca ccgcgcctga aattcgagac gggcgacgcg
      721 ttccatctgg cgcagccgga caacaccttc gacctggtgg cgaaccggca catgatccag
      781 tcggttccgc acgtggaccg catcctcgcg gagctggttc gggtcacccg gcctggtgga
      841 cgtgtacatg tgctcgccga ggactattcg atgctgcaca tgatgtcagg gccactcgac
      901 cccgacgtct tctggcgcaa aggccccgtg gaattctcca tgcgtactga cacggattcg
      961 cgcatcggcc gccgcgcctg gacgctcatg cgcgaggccg ggctgatcga cgtgcgagtg
     1021 gactacgtgg tggtggatac gctgcgcgtg ccgcggcgcg tgttcgcgga catcatcacc
     1081 gcgtggcgcg acggctatgt gaacgcgttg gcgacgcccg aatttcccgc agcaacggtc
     1141 cgccagcact tcaacgaaat tatcgccagc atattgaatc cggatcacta cgcggtctgg
     1201 catataccga ttgtttcagg aatgaaaccc agggccccat aaaaaagcgg cggacccatg
     1261 gggcccgccg cctttgttca acacgcttga aggatcagcc tgcagtgatg ggctgcagct
     1321 tgagctcgac gcggcgattc tgctgccggc cttcggcact gtcgttcgag gcaaccggac
     1381 ggctctcgcc ggcacccacc gtcacgatgc gctggctgtt cacgccatgc gactgcagat
     1441 agctgcccac cgagccggca cgctgtaccg acaggttctg gttgtagctg tcgtcaccca
     1501 cgttgtcggt gtggccttcg acgtcgatga gcgtcttgtc gaattccttg aggacgatcg
     1561 tcaccgaatc gagcaccttg tagaagcccg cgttcagatc cgagctcgcg gtcttgaacg
     1621 tgatgttccc gggcatgttc agaatgaggt cattgccatt gcgcgtgacg gacacgcccg
     1681 tgccctggag ctgcgcgcgc aacttggctt cctgcttgtc catgtaggcg ccaatgccag
     1741 cacccgagag cgcaccgacg cctgcgccga tgagcgcgcg ttcgcggcgt tccttggagt
     1801 tgtcccccgt ggcggcgccg atgacggcac ccgcgatggc gccgatgccg gcacccttgg
     1861 aagcgttgtt caccttcttc tcgccggtgt aggcgtcggt ggtctggcag cccgcgacgg
     1921 tgaaggcggc ggcgatggct aacaatgtgg tgcgtacgaa aatcttgttc atgcagttct
     1981 cctgaatcgt cgggcaaaca taacggcgtg catctgaacg ccgaatgaac cgggtttctg
     2041 tgagccagtt ctcagtcgac ggctctattc cacgttttcg ggcgatgccg ggggcgtggt
     2101 tgcgcgcttg tagccgcgcg gtcgaatcgc aaccgtgttg cgaattaccg tgcagcacac
     2161 gccatcgttg cggacatagc cgtattcgaa cgtcggggta gcacgccctt cgttttggag
     2221 ccctatccta atgtcctgct cctgagcagg tgtaagcgca aaccgcatct cgaggtcgga
     2281 cgtcccgccg tgatcgaagg aaatcgacat ggcgcgcgtc cacaccgagt agcccgggaa
     2341 aacccgagcg catgccagcg ccgcgatggg atcggcgagc gccgcctggt agccgccgaa
     2401 catgacgccg ccgggattgc gcgaccgcgc gttgagcggc agcaggatgc gcaccgtgcg
     2461 ccagttttcg gagatctcga gcaccttgat gcgcatgggc acgaacggcg gatacagctc
     2521 caaaagccgc gcgtcgggca ggatcctgct gcgggccacg aacttcatga ggcgctgcat
     2581 cagcggcatg tcttcttcct tgtttgacaa gccgtcattc ttgcagaggc cacgacgcag
     2641 gcgtagcgtt ccgcgtcatg cgcaccgtcg agcaatggtt tggggaatat ggcgaaagcc
     2701 acagcaatcc tcgcaatgaa ttgctgcaca tcatctgtgt gccgcccatc gtgctcaccg
     2761 tcatcggttt catctgggcc atcccggtac ccgccgcatt cgcggacgtt tccccctggc
     2821 tcaactgggc cacgatcacc atcgcgctcg ccgtcgtcta ctacttcacc ctgtcggtga
     2881 agctcggcat cggcgcggcg attgcgctcg gcatcctggc actcatcgtg agctggctgg
     2941 ataccctcgc ctggccactg tggctcacat gcctggtcat ttttgccatc ggctgggtcg
     3001 gccagttcat cggccacgcc atcgaaggca agcggccttc atttttcaag gatctgcagt
     3061 tcctgctgat cggcccgctc tggctgctcg ggcatctgta tcgtcgactg ggcatcaact
     3121 actaggtgcc gggtggggag tggggagagc cgctctcccc actccccacc cggcaccgta
     3181 tcatcgcggg atgttgaatc ccctcgatct ctatgacatc cgcggtctgc tgaccgaaga
     3241 tgaacgcatg gtgc
//
LOCUS       DASQJS010000317         4253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_498229, whole genome shotgun sequence.
ACCESSION   DASQJS010000317 DASQJS010000000
VERSION     DASQJS010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498229"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VEW08_12775"
     CDS             <1..496
                     /locus_tag="VEW08_12775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ41658.1"
                     /translation="RRMPFDAAACEDVAQATLCRALESLHGYRGEAALMTWLCTLCRR
                     ELSARWRENQGWAAAPMLAEDDPEIRAALESLMTSEQGDPLLATHREEVRQSILITLD
                     YLPAPYAEILECKYLRDMSIGEIARQMGRSPKATESLLTRARDAFREAFALLPGGTRG
                     ALTP"
     gene            493..1479
                     /locus_tag="VEW08_12780"
     CDS             493..1479
                     /locus_tag="VEW08_12780"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HYJ41659.1"
                     /translation="MSHPESERSIERLIKLAGGRDMPTAEAMERARLAAHESWSRMLK
                     QPAVPRRARLRTMLGFALAAGIAAFGILLWTQRPAPAAPELVARIATLTGDAQLHAAH
                     AETSARVALPIHAGTTLATNEGRVAFTFGDSLSLRIDRHTRLRFDGREQVTLLAGALY
                     VDSGGINAVSALRIETPAGAVRHVGTQFQVHVTGDVTIVRVREGRVLLTRASGTPTDI
                     AAGDELRVRGGELRLQRGLPSFGEDWEWSAGIAPALEIENRPLAEFIAWLAREHGWQV
                     RYADESLQARTFDIRLHGSLDQMESAAMLERVTLVTGVPLQAGNGVLWVGGK"
     gene            1476..3821
                     /locus_tag="VEW08_12785"
     CDS             1476..3821
                     /locus_tag="VEW08_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ41660.1"
                     /translation="MKRIRSTWVAMLVALAGGAWAAESPALNAPLHAGATLESALAQL
                     NARGYRIVYSNALVQPSMTLRESPKSTRIDDLLREILAPWKLRAVLASNGDWLVTSEA
                     TPTTSIESEIVDDHFGSIDTIDVTASRVRLATAGASETFLDREDVQRMPHLADDAVRM
                     LKVLPGVTGGDFSAALNIRGGRREEALLTVDGAEIHNGFHFRDIDGALSVLDTHLVEG
                     IDFITGGMTAEYGDFMSGVVGLQSRRPSADDEYRSGVGISFVSLYGRTSGNFADDRGS
                     WLVSARRGFLDVLTERVVDDNEQLTPRYTDVFASTDYDLSERSSLTARLLMSQDDLKF
                     ATDDAADDIDSAGKGHSTHLWLGFDHFWTDALQMNTLVSVATVNQTRDANGIDDQRFG
                     DVRSDNDFRFLDLKQDWSWQLNDRHLPRWGFNVNRQEGEYDYALVSRIMDPLVTPVPV
                     DVAYGTNMDVHVNKLGAYASWRARLTDAITAEAGARWDSYEYPDELKFDVVSPRLNVV
                     YEFGNDNELRAAWGVVYQPQAVNELQVEDDVERFFEPERSEQFVVGYTRHFTRGLSLR
                     VDVYDKDYSDLRPRFENLLDPIQLIPEGAADRIRVDAPEARARGVELTIRREAERGLS
                     GWVSLSIAEAEENVAGEWQSRGWEQRQTLAFGSSWTGAKWNVSLAGLFHSGTPTTYIG
                     IETTPLPGGGTEVGGVVGQRNAEHMAAYTRVDLRANRDVQLRNSRISFYLEVTNLLNS
                     RNECCVENYHLEQGSNGGYFLDTEIGYWLPMLPSFGFQWEF"
     gene            complement(3818..>4253)
                     /locus_tag="VEW08_12790"
     CDS             complement(3818..>4253)
                     /locus_tag="VEW08_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41661.1"
                     /translation="FHAGFASNTAHKLVEGLATLVKERRLKSALPFARILYTLGRWLQ
                     PVFSDRGAMFVKLEGLHDNGARYSHTWNIVARENHGPNIPCAAAIALANKIAAGGKLP
                     AGAMPCMGLLSVEELMIPLKGFSIREFPPFGPGGVELGKLET"
BASE COUNT          764 a         1351 c         1402 g          736 t
ORIGIN      
        1 gcgccgcatg ccgttcgacg cggcagcctg cgaagacgtg gcccaggcca cgctgtgccg
       61 cgcgctcgag agcctccatg gctatcgcgg cgaggcggcg ctcatgacct ggctttgcac
      121 cctctgccgg cgcgagctct ccgcgcgctg gcgtgagaac cagggctggg ccgcggcgcc
      181 gatgttggcc gaggacgatc ccgagatccg cgcggcgctg gaatccctga tgacgagcga
      241 gcagggcgac ccgctgctcg ccacgcatcg cgaggaggtg cgccagtcca tcctgatcac
      301 gctcgactat ctaccggcgc catacgccga aatcctcgaa tgcaaatatc tgcgcgacat
      361 gagcatcggc gagatcgcgc gccagatggg acgcagcccg aaggccaccg agtccctgtt
      421 gacgcgggcg cgcgacgcat ttcgcgaggc atttgcgctg ctgcccggtg gtacgcgcgg
      481 agcactgacg ccatgagcca tcctgaatcc gaacgttcga tcgaacgcct gatcaagctt
      541 gccggcgggc gcgacatgcc aaccgccgaa gcgatggagc gtgctcggct ggcggcgcac
      601 gaatcctgga gccgcatgtt gaaacagccc gccgtgccgc gccgagcgcg gctcaggacg
      661 atgctggggt tcgcgctggc ggcgggtatc gccgcattcg gcatcctcct gtggactcag
      721 cgtccggcac ctgcagcgcc cgagctcgtg gcgcgcattg cgacgcttac gggcgatgcc
      781 cagctgcacg cggctcacgc cgaaaccagc gcacgcgtgg cgttgcccat tcacgcgggt
      841 accacgcttg ccacgaatga aggccgcgtc gcgttcacct tcggtgactc gctttcgctg
      901 cgcatcgatc gccacacgcg cctgcgattc gacggccgcg aacaggtaac gctgctcgcg
      961 ggcgcgctgt acgtcgattc cggcggcatc aatgcggtgt cggcgctgcg catcgaaact
     1021 cctgcaggag cggtgcgcca cgtcggcacg caattccagg tgcacgtgac cggcgacgtc
     1081 acgatcgtgc gcgttcgtga agggcgcgta ttgctgaccc gcgcttcggg cacgcccacg
     1141 gacatcgccg ccggtgatga acttcgggtt cggggcgggg aactgcgatt gcaacgggga
     1201 ctgccgtcgt tcggagagga ttgggagtgg tcggcaggca tcgcgccggc actcgaaatc
     1261 gaaaaccgcc cgctggccga attcatcgcg tggttggctc gagaacacgg ctggcaggtc
     1321 cgctacgctg acgaatcgct gcaggcgcgc acattcgaca tccgcctcca cggctcgctc
     1381 gatcaaatgg aaagcgcggc gatgctggaa cgcgtgacgc tggtcaccgg ggtaccgctt
     1441 caggcaggaa acggcgtgtt gtgggtgggc ggaaaatgaa gcgcattcgc agcacgtggg
     1501 tggccatgct ggtcgccctg gccggcggcg cctgggccgc ggaatcgccg gcattgaacg
     1561 caccgctgca cgccggagcg acgctcgaat ccgcgcttgc gcagctgaac gcgcgcggct
     1621 accggatcgt ctacagcaat gcactcgttc agccttcgat gaccctgcgc gaatcaccga
     1681 agtccacgcg catcgatgat ctgctgcgcg agattcttgc accgtggaaa ttgcgcgcgg
     1741 tgcttgcatc caatggcgac tggctggtca ccagcgaagc aacgccgacg accagcatcg
     1801 agtccgagat cgtcgacgat cacttcggga gtatcgacac catcgacgtc acggccagcc
     1861 gcgtgcgtct cgccaccgcg ggcgccagcg agacattcct cgatcgcgaa gacgtccagc
     1921 gcatgccgca tctcgccgat gacgcggtgc gcatgctcaa ggttctgccg ggcgtgaccg
     1981 gtggggattt ctccgcggcg ctcaacatac gcggcgggcg gcgcgaagaa gcgctgctca
     2041 ccgtcgatgg agcggagatt cacaacggat ttcactttcg cgacatcgat ggcgcgctct
     2101 ccgtgctcga tacccatcta gtggagggca tcgacttcat caccgggggc atgaccgccg
     2161 aatacggcga cttcatgagt ggcgtggtcg gcctgcagtc caggcgtccg tcggccgacg
     2221 acgaatatcg cagcggagtc ggcatcagct tcgtcagtct ctacggccgc acctccggca
     2281 actttgcgga cgatcgcggt tcgtggctgg tgtcggcgcg gcgcgggttc ctcgacgtgc
     2341 tcaccgagcg cgtcgtggac gacaacgagc agctcacgcc gcgatacacc gatgtctttg
     2401 cttccaccga ctacgatttg agtgagcgca gctcgctgac cgcgcgcctg ctcatgagcc
     2461 aggacgatct gaagttcgcg accgacgatg ccgccgacga catcgattcc gccggcaagg
     2521 ggcactccac gcatttgtgg ctcggattcg accacttctg gacggatgcc ctgcagatga
     2581 acacgctcgt ttccgtggcc accgtcaacc agacccgtga tgcgaatggc attgacgacc
     2641 agcgtttcgg cgacgtacgc agcgacaacg atttccgttt cctggatctg aagcaggact
     2701 ggtcctggca gttgaacgac cgtcacctgc cacgctgggg gttcaacgtg aatcgccagg
     2761 agggcgagta cgactacgcg ctcgtgtcgc gcatcatgga tccgctggtg acgccggtac
     2821 cggtcgacgt cgcctacgga acgaacatgg acgtgcacgt gaacaagctt ggcgcctatg
     2881 cctcgtggcg cgcgcggctg actgatgcca tcacggccga agccggcgcg cgctgggaca
     2941 gctacgaata tcccgatgaa ctgaagttcg atgtggtgag cccgcggctc aacgtggtgt
     3001 acgagttcgg caatgacaac gaattgcgtg ccgcgtgggg cgtcgtgtac cagccgcagg
     3061 cggtcaacga actccaggtg gaagatgacg tggagcggtt tttcgaaccg gagcgctcgg
     3121 agcagttcgt ggtgggttac acgcggcatt tcacgcgagg gctgtcattg cgcgtcgatg
     3181 tctatgacaa ggactacagc gatctgcggc cgcgtttcga aaacctgctc gacccgatcc
     3241 agctgatacc cgaaggcgcc gccgatcgca tccgcgtcga tgcgccggaa gcacgggcgc
     3301 gcggtgtcga actgacgatt cggcgcgagg ccgagcgcgg cctttctggc tgggtgagcc
     3361 tgtcgatcgc cgaagccgaa gaaaacgtgg ccggtgagtg gcagtcgcgc ggctgggaac
     3421 agcgccagac gctggcattc ggcagcagtt ggacgggtgc gaagtggaac gtgagcctgg
     3481 cggggctttt ccacagcggc acaccgacga cctacatcgg catcgagacc acaccgctgc
     3541 ccggcggcgg caccgaggtg ggaggggtgg tggggcaacg caatgccgaa cacatggcgg
     3601 cctatacgcg cgtggacctg cgggccaacc gcgacgtgca gcttcgcaac agccggattt
     3661 ccttctacct ggaagtgacc aacctgctca acagcaggaa cgagtgctgt gtcgagaact
     3721 atcacctcga gcagggaagc aatggcggat attttctcga caccgagatc ggctactggc
     3781 tgccgatgct gccgtcgttc ggctttcagt gggagttcta ggtttccagc tttcccagtt
     3841 ccacaccgcc aggtccgaac ggcgggaact cccggatgct gaaccccttc agcggaatca
     3901 tcagctcttc cactgacaac agacccatgc aaggcattgc gcccgcgggg agcttcccgc
     3961 ctgctgcgat cttgttggcg agggcaatcg ccgcggcgca cggaatgttt ggcccgtggt
     4021 tctcgcgggc cacgatattc cacgtatgtg agtaccgggc gccgttgtca tgcagtcctt
     4081 cgagtttcac gaacatggcg ccccggtcac tgaacacggg ctgcagccag cggccgagcg
     4141 tgtacaggat gcgtgcgaac ggcagcgcgc tcttcagtcg ccgctctttc acgagcgtcg
     4201 ccagaccctc gacgagcttg tgagcggtat tgctggcgaa gccggcgtga aag
//
LOCUS       DASQJS010000318        10427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3961435, whole genome shotgun sequence.
ACCESSION   DASQJS010000318 DASQJS010000000
VERSION     DASQJS010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10427
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3961435"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3984)
                     /locus_tag="VEW08_12795"
     CDS             complement(<1..3984)
                     /locus_tag="VEW08_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41662.1"
                     /translation="MNTLIRAGLIALAIFCFRAAHAQEGIPPALREWQAWVLHGEEFR
                     RCPFTASAAVEPGEPIDESEFRCVWPERLTLAVDARGGTFSQRWQVYSESWVQLPGDS
                     ENWPREVRLNGAPAAVVAVDDAPNLRLTPGSYTLSGRFEWSSRPESLALPDATAIVDL
                     FVDGSRVAQPERPDGGVWLGKRRSTEQAAAMEVQVYRLVQDEIPAYLLTRIRLNVAGE
                     AREEVLGRALPDGFTPLSLQGDLPARLERDGTLRVQLRAGSHEIFLYARGTDVAQTLS
                     RPENSSGKWPKEEVWSFAPNDSLRVAAAEGAEGIDPAQANVPAEWRQYPAFRMDASAK
                     LTVVERSRGLSNADDNRLTLSRAIWLDFDHRGFTAVDQVNGLMRRNWRLDMQPPFALQ
                     SARQGNDQLLVTEGEKGRAGVELREPRLNLTTVARKETGSGAMPATGWDSRFDRVSGT
                     LNLPAGHRLIAAIGTDGAPGAWWERWGLWNVFGVLLIVGFVYWTGGLIPAAIAALALL
                     LTYQELPEFIWLWGNLLAALAIARAAPAGRFQKFASAYRTVSFVVLGIALLPFLWMQI
                     RYALYPQLAPSDASYGIMANARGQYAPTMEAKQEAYADAAAPAADAAAPASVEADAIT
                     AEDIGKLPDSNVRESLQRVPGVSPSAGYLNSVQVVQRYAAGTVLQAGPGIPAWGYNSY
                     SYYWSGPVESADTVRFIYVGPVVLFFWRLLGVFALAALFAWLALLSYGKRVSLPGFPK
                     PASATALLPGLLASSLLLLSLGMGTPAHAASEQGPDAPANDLLTELKTRLTAAPKCAP
                     DCAEISAARVTVEGDRLEVVLQVSALASVAVAMPHASDRWQLDDVSVDARGTLAMARD
                     DDASLWVPLTAGAHTVRLAGRLAAAESIQLAFPQRPRVIDVSARGWTTSGVNEGRLVS
                     GSLELARERDAERSDAALEAGAEFPAFVRVDRLFSLDLDWTLDTIVTRIAPQRAALSV
                     EIPLVKGESVLTDGVKVRNNEAALVGLGAGERQMQWHSGLARAETLEVSVPADAARSE
                     VWSFFVNPQWNVVFEGFAPVLPENVNASSWVFRFMPRPGEKLTLKVTRPKGAKGTTLA
                     IDSASLHTTIGSRSSSTTLQFAYRSTQGGRHVIQLPEKARVTGVTFDGRPQQLRPEKG
                     ELPLSLAPGEHSLLISWEESRDVSLLTHPSTVDLRSAASNLTFGVALPDSRWVLAAWG
                     PGIGPAVLYWGELVVFIVVAWLLGRWANSPLRFTEWLLLGLGLSTQSWFVFCLTAAWL
                     IAMRWREGWKPGEEFLRWRFNAVQVVLAVFTFITILTLVFSGIRNGLLAYPDMHITGR
                     DWQGGGYT"
     gene            4065..4685
                     /locus_tag="VEW08_12800"
     CDS             4065..4685
                     /locus_tag="VEW08_12800"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HYJ41663.1"
                     /translation="MHSAGDILEYWFGTGDWTPARLTERTTFWFGGDGAAAAAARDAD
                     IRARLEPLLERASRGEFAGWASSPKRRLALIILFDQVPRNAYRGTAAAFAFDREALAL
                     AVEGLQLAADAALYPIERIFFYLPLEHAESLEAQDAAIAAFERLIAEAPADLRDYCAY
                     TADYARKHRDVIAQFGRFPHRNEILNRPSTSAEIEWLASKGERFGQ"
     gene            complement(5033..5941)
                     /locus_tag="VEW08_12805"
     CDS             complement(5033..5941)
                     /locus_tag="VEW08_12805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HYJ41664.1"
                     /translation="MSDHGSSAKAILYAFIANLGIALAKLAASIYTNSGSMMAEAIHS
                     FADSGNQVLLYLGIKQAEKPPDARHPLGYGRITYFWSFIVALLLFSMGGLFSIYEGWH
                     KLHAPEELNKAWVALVVLGVSIGLEFGSLLGCLREIKKLRKGKSLGHWIKHTRNAELV
                     VVLGEDLAALLGLMLAFVFVALAASTGNPVFDAAGSIVIGVILICVSIFIAVRIKGLI
                     VGRSAEEDLQEALRTEVAASPDIEKLLNAITLQMGPDVMLALKVQMRAGLTLEAAVDS
                     INELERRIKAKFPEVAWCFVEPDVAD"
     gene            complement(5941..7392)
                     /locus_tag="VEW08_12810"
     CDS             complement(5941..7392)
                     /locus_tag="VEW08_12810"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYJ41665.1"
                     /translation="MKRHMLKNAAARVAALLLGGALAFSGAAIGADSTAAVGVKVPAN
                     QRFVLPNGLTIVLVPRKDVPLIAFSGFIRGGVLADPADKPGVSSLVAGLLDRGAGKRN
                     AFEFADAVAGVGGSFNATAGSESISVNGQFLARDRALMIELLADALMRPRFDAQEFSS
                     YRDRDIEFIKSAKDSDPSQIVGIYGRAALFGSHPYGRPQGGSERTLAAITHGDVVSFH
                     AGNFGADRATLVFAGDLDPKWMKQALTAAFGGWGKAKAPAVALKPAPRVTGRRVVLVD
                     SPGSVQTYFWLGNVGVDRRYSGRPALDLVNTLYGGRFTSILNTELRIKSGLSYGASSR
                     FTRGTVPGEFAIRSFTQTENTAKALDLTLETLEKLKHDALAPDMLESARSYVLGQFPL
                     QLETAAHWAVTLADLELYGLPKDYIEGYGPALARVDLAETAAVTADAFPRPADLVIVL
                     VGDAAKIREAAAKYGPVTEMKISDPDFAPAKLP"
     gene            complement(7389..8738)
                     /locus_tag="VEW08_12815"
     CDS             complement(7389..8738)
                     /locus_tag="VEW08_12815"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYJ41666.1"
                     /translation="MPNSFVVPALCACVLFFSASSSAATKPTAPVRVPGLDQVQATTL
                     ANGMKVIVWTDRDIPNVALYNWVRVGSRNEVPGITGLAHFFEHMMFNGTARRTPGEFD
                     RIMEARGGSNNAFTSENVTVYQDWIPRTALDLVFDLESDRLANLSFDPKVVESERGVV
                     YSERRLSVEDNNAGFLVEQVQATAFVAHPYQFPTVGWPADIQGWKIEDLQKFFKTNYA
                     PNNCTLIMVGDLSAEEGFALAKKYLEPIPRQEPPPPVRTVEPEQLGEKRVFVQREAST
                     PLLYYAYKSPAANDPQGPAINLLMSVLTEGDASRLHRLLVEEKRLAIEVDGSFQEGFD
                     PGLSWVQLTLPEGANVAEVEKVLDAALDQVVAQGVTDAELARAKNLYAAAFWKQIATI
                     NGKALLLGSFDVFEGDYRKLFDSPAAYDKVTREDVQKAAALVLQKNHRTVGVLEAKP"
     gene            complement(8889..9071)
                     /locus_tag="VEW08_12820"
     CDS             complement(8889..9071)
                     /locus_tag="VEW08_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41667.1"
                     /translation="MARSTVTLILPHAQGVASMRPALAGMLVKRRRGGSVMFAPIDAR
                     LARAQLTAIHAVKVSR"
     gene            complement(9263..9946)
                     /locus_tag="VEW08_12825"
     CDS             complement(9263..9946)
                     /locus_tag="VEW08_12825"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYJ41668.1"
                     /translation="MSTRALVTLFAIPLLALAACSREKSDWRSAQAADSPESYEQFIA
                     GHPDSTLVATARERLQQLAEEKDWRAAAQTDSLEAYQQFLAQYPAGKWAKEAQVRIDN
                     FASADTAAAVAGAGSSAGATPPVAAPSGEGASLPDTPASTPVASGYGVQLGAFSSAER
                     ANEEWKKLQADSAGLLDGLSPRVVVGEAQGKAIYRLQAEVRDEVQARAICGGLKVAKK
                     PCVPVIPHP"
     gene            10007..10405
                     /locus_tag="VEW08_12830"
     CDS             10007..10405
                     /locus_tag="VEW08_12830"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HYJ41669.1"
                     /translation="MSEEPKRRLVDRLGMSPTVIAENTTVVGDIETRGPLMVSGNVQG
                     NGTIGGTLSVGKSAHWVGDISARQAVLAGKVTGRIVVEDKLEISASAVISGEIVAKIL
                     AIANGARIEGEVTVTSGKPILKFEEKRAAG"
BASE COUNT         1884 a         3403 c         3390 g         1750 t
ORIGIN      
        1 cgtgtagccg ccaccctgcc agtcgcgccc ggtgatgtgc atgtcgggat acgccagcag
       61 gccattccga atgccggaga acaccagcgt caggatggtg atgaacgtga acaccgcgag
      121 caccacctgc acggcgttga atcgccagcg caggaattcc tctcctggct tccagccttc
      181 acgccagcgc atcgcgatca gccacgccgc ggtcaggcag aacacgaacc acgactgggt
      241 ggataacccg aggccgagca acagccactc ggtgaatcgc aatggtgaat tcgcccatct
      301 tccgagcagc cacgccacca cgatgaacac cacaagttcg ccccagtaga gcaccgcagg
      361 accgatgccc ggaccccagg ccgcgagaac ccagcgcgag tcgggcagcg cgaccccgaa
      421 cgtcagattg ctcgccgccg accgcaggtc gaccgtcgac ggatgcgtca gcaagctcac
      481 gtcacgcgat tcttcccagg agatcagcag actgtgctcg ccgggcgcca gcgatagcgg
      541 taactcgcct ttctccggac gcagttgctg cggccggccg tcgaacgtca cgccggtgac
      601 gcgcgctttc tcgggcagct ggatgacgtg ccgtccaccc tgcgtgcttc gatacgcgaa
      661 ttgcagcgtc gtgctcgacg agcgcgaccc gatggtggtg tgcaggctcg ccgagtcgat
      721 ggccagcgtc gtgcctttcg cgcccttcgg ccgcgtgact ttcaacgtca gcttttcacc
      781 cggccgcggc atgaatcgaa acacccacga cgacgcgttc acgttttcgg gcaacaccgg
      841 cgcgaagcct tcgaagacca cattccactg cggattgacg aagaagctcc acacttcgct
      901 gcgcgccgcg tccgcgggca ccgaaacctc gagcgtctcg gcacgtgcca ggcccgagtg
      961 ccactgcatc tgccgttcgc cggcgcccag ccccacgagt gcggcctcgt tgttgcgcac
     1021 tttcaccccg tcggtgagca ccgattcgcc cttcaccagc ggtatctcga ccgatagcgc
     1081 ggcgcgttgt ggcgcgatgc gcgtgacgat cgtgtcgagc gtccagtcga gatccaggct
     1141 gaacaggcga tcgacgcgaa cgaatgccgg aaactcggcc ccggcctcga gtgcggcatc
     1201 gctgcgttcg gcgtcacgtt cgcgtgccag ctccagcgag ccggatacca gccggccctc
     1261 gttgacgccg ctggtggtcc agccgcgcgc gctcacgtcg atgacgcgag gacgctgcgg
     1321 gaatgcgagc tgaatggatt cggccgcggc gaggcgaccg gcgagccgca cggtgtgggc
     1381 gcccgcggtg agcggcaccc acagcgacgc gtcgtcgtcg cgcgccatcg ccagcgtgcc
     1441 gcgtgcgtcc acgctgacgt cgtcgagctg ccagcgatcg ctggcgtgcg gcatggccac
     1501 cgcgacggag gcaagcgcgc tgacctgcag caccacttcg agccggtctc cttccaccgt
     1561 cacgcgcgcc gcggaaatct ctgcgcaatc gggcgcgcac ttgggcgcgg ccgtgaggcg
     1621 tgtcttgagc tcggtgagca gatcgttggc tggcgcatca ggaccttgtt ccgacgcggc
     1681 gtgcgcgggc gtgcccatgc cgaggctcag tagcagcagg gacgacgcca gcaatccggg
     1741 cagcagcgcc gtagcggagg cgggctttgg aaacccgggc agcgaaacgc gcttgccata
     1801 gctgagcaac gccagccacg cgaacagcgc ggccagcgcg aacacgccga gaaggcgcca
     1861 gaagaacagc actacgggcc ccacgtagat gaagcgcacg gtatcggcgg actcgaccgg
     1921 accactccaa tagtaggaat aggagttgta tccccaggcg ggaatgccgg ggccagcctg
     1981 cagcaccgtg ccggcggcat aacgctgaac cacctgaacc gaattgaggt agcccgccga
     2041 gggcgaaacg cctggcacac gctgcaggct ttcgcggacg ttggagtcgg gcaacttgcc
     2101 gatgtcttcg gcggtgatgg cgtcggcttc cacggaagcc ggggcggcgg catcggctgc
     2161 cggcgcggcc gcgtcggcgt aggcctcctg cttcgcctcc atggtcggag cgtattggcc
     2221 gcgcgcgttc gccatgattc cgtagctggc atcgctgggt gccagttgtg gatacagcgc
     2281 gtagcgaatc tgcatccaga ggaatggcaa cagcgcgatg cccagcacca cgaaactcac
     2341 cgtgcgatag gcgctcgcga acttctggaa gcgccccgcg ggcgcggcac gtgcaatggc
     2401 gagcgccgcc agcaggttgc cccacagcca gatgaactcg ggcaactcct ggtaggtgag
     2461 caacaacgcg agcgcggcga tcgccgccgg tatcagtccg cccgtccagt acacgaagcc
     2521 cacgatcagc agcacgccga acacgttcca caggccccag cgctcccacc acgcgcccgg
     2581 cgcaccgtcg gtgccgatcg ccgcgatcag gcgatggccg gctggcaaat tgagcgtgcc
     2641 cgagacgcga tcgaagcggc tgtcccagcc ggtcgccggc atggcgccgc ttccagtctc
     2701 cttgcgggcg acagtggtta gattgagccg cggctcgcgc agctccacgc cggcgcggcc
     2761 cttctcgcct tcggtcacca ggagttggtc gttgccctgg cgcgcgctct gcagcgcgaa
     2821 cggcggctgc atgtcgagcc gccagttgcg tcgcatgagg ccgttgacct gatcgacggc
     2881 ggtgaagccg cgatggtcga aatcgagcca gatggcgcga gacagcgtga gccggttgtc
     2941 gtcggcattc gaaagaccgc ggctgcgctc caccacggtg agtttcgcgg aagcgtccat
     3001 gcgaaatgcc gggtactggc gccattccgc gggcacgttg gcctgcgcgg gatcgattcc
     3061 ctcggcacct tccgcggcgg cgacacgcag cgagtcgttg ggggcaaagc tccacacttc
     3121 ctctttgggc catttgccgc tgctgttctc cggccgggac agcgtctgag ccacgtccgt
     3181 gccgcgcgcg tacaggaata tctcgtggct gccggcgcgc agctggacgc gcaacgttcc
     3241 gtcgcgttcg aggcgagccg gcaggtcgcc ctgcaacgac agcggcgtaa agccatcagg
     3301 cagcgcgcgg cccagcacct cttcgcgcgc ctcgcccgcc acgttgaggc ggatgcgcgt
     3361 cagtagatag gccgggatct cgtcctgtac cagccggtag acctgcacct ccatggcggc
     3421 ggcctgctcg gtgctgcggc gcttgcccag ccatacgccg ccatcgggac gctccggttg
     3481 ggcgacgcgt gacccatcca cgaacaggtc gacgatggcg gtggcgtcgg gcagcgccag
     3541 cgactcgggg cgcgagctcc actcgaagcg gccggaaagc gtgtagctgc cgggagtgag
     3601 gcgcaggttg ggcgcatcgt ccacggccac gaccgcagcg ggcgcaccgt tgagccgcac
     3661 ttcgcgcggc caattctcgg aatcgccggg cagctggacc cagctttcgg aataaacctg
     3721 ccagcgctgg gagaaggtgc cgccacgtgc atcgacggcc agcgtcaggc gctccggcca
     3781 gacgcagcgg aattcacttt catctatggg ctcgccgggt tccacggccg cggaagccgt
     3841 gaaaggacag cgccggaact cttcaccgtg caacacccag gcctgccatt cgcgcagtgc
     3901 tggtgggatg ccctcctggg cgtgcgcagc ccggaaacag aagatagcga gcgcgatcag
     3961 ccctgcgcgg atgagcgtat tcattcttgt gccctctacc cctggccggt cctcagtgta
     4021 acaacgtcgt tagacgctcg ttcggtctgg catccttggc gccaatgcac agcgctggcg
     4081 acatactcga atactggttt gggactggcg actggacgcc ggcgaggctc acggaacgca
     4141 ccaccttctg gttcggcgga gacggcgcgg cggccgcggc ggcccgcgac gcggacatcc
     4201 gcgcccggct cgaacccttg ctcgagcgcg catcgcgcgg cgagttcgca ggctgggcgt
     4261 ccagccccaa acgccgcctg gcgttgatca ttctgttcga ccaggtgccg cgcaacgctt
     4321 accggggcac ggctgccgcg ttcgcgttcg atcgtgaagc attggcgctc gccgtcgagg
     4381 gcctgcagct cgccgccgat gcggcgctct atcccatcga gcgcatcttc ttctatctac
     4441 cgctggagca cgccgagtcg ctggaagcgc aggatgcggc catcgccgca ttcgagcggc
     4501 tgatcgccga agcgcccgct gacctgcgcg actactgcgc gtacaccgcg gactacgcgc
     4561 gcaagcaccg ggacgtcatc gcacagttcg gccgctttcc gcaccggaat gagatcttga
     4621 atcggccgag cacgtccgcg gagatcgagt ggctggcgtc gaagggagag cggttcgggc
     4681 aatagtcttc aatagatttt tccgaaaaat gtcgctccga gattcacgcg cgaacaacag
     4741 gtacgcaagc gtgaacacca gcagaatcag gaatgtgtag ttggctaatt cacccaattg
     4801 atcggcagcg gtacccaagt attccgccgc ggcatgcgaa cgggcatcgc cctgcgcctc
     4861 tgcccaatat cggcttttgt attgcaggtg ccagccaagg gcggcgtggt cggcagcctc
     4921 gctgtacagc gcggtgagag ccagcagcaa cagcggcagg agaagccgcg cgatccattc
     4981 cctgacgttg aatgccatcg ccgaataaac gcacggcagc gcacttcggg tctcagtcgg
     5041 cgacgtcggg ctccacgaag caccaggcga cttcgggaaa ctttgccttg atgcgccgct
     5101 cgagctcatt gatggagtcg acggcggctt cgagcgtgag tccggcgcgc atttgcacct
     5161 taagcgccag catcacgtcc gggcccattt gcaaggtgat ggcattgagc agcttttcta
     5221 tgtcggggct ggcggctact tcggtgcgca gcgcttcctg gaggtcctct tcggccgaac
     5281 gcccgacgat cagccccttg atacgcacgg cgatgaagat cgacacgcat atcaggatca
     5341 caccgatgac gatcgacccc gcggcatcga acaccgggtt gccggtggac gcggccagcg
     5401 cgacgaatac gaacgcgagc atcaggccca gcagcgcggc cagatcttca ccgagcacca
     5461 caaccagctc cgcgttgcgc gtgtgcttga tccagtggcc cagcgacttg cccttgcgca
     5521 gcttcttgat ctcgcgcagg cagccgagca gtgatccgaa ctcgagcccg atcgacacac
     5581 ccaacaccac cagcgccacc caggccttgt tgagctcctc gggcgcgtgc aacttgtgcc
     5641 agccctcata gatcgaaaac aggccgccca tcgagaacag cagcagggcg acgatgaagc
     5701 tccagaaata cgtgatcctg ccgtatccca gcgggtgcct ggcatcgggt ggcttctcag
     5761 cctgcttgat gcccaggtac agcagcacct ggttgccgct atcggcgaag gagtgaatcg
     5821 cctcggccat catgctgccc gagttcgtgt agatagacgc cgcgagcttg gcgagcgcta
     5881 tgccgaggtt ggcgatgaag gcatagagga tcgccttggc ggaggaaccg tggtcggaca
     5941 tcagggcagc ttcgcgggcg cgaaatccgg atcggagatc ttcatctcgg tgaccggacc
     6001 gtacttggcg gcggcttcgc ggatcttggc cgcgtcaccg acgagcacga tcaccagatc
     6061 tgcagggcgc gggaaggcgt cggcggtcac cgcggcggtt tcggccaggt ccactcttgc
     6121 gagcgccggt ccgtaacctt cgatgtagtc cttgggtaac ccgtagagct cgaggtcggc
     6181 gagcgtcacc gcccaatgcg cagcggtttc gagctgcagc gggaactggc cgagtacgta
     6241 tgaccgtgcg gactcgagca tgtccggcgc aagcgcgtcg tgtttcagtt tttcgagcgt
     6301 ctcgagtgtg agatccagcg ccttggcggt gttctccgtc tgcgtgaacg agcggatcgc
     6361 gaattcaccc ggcaccgtgc cgcgcgtgaa gcgcgaggag gcgccatacg agagcccgga
     6421 tttgatgcgc aactcggtgt tgagaatgga agtgaaccgg ccgccataca gcgtgttcac
     6481 gaggtcgagc gccggccggc cgctgtaacg cctgtctact ccgacgttgc ccagccagaa
     6541 ataggtctgc accgatcccg gtgagtccac gagcacgacg cggcggccgg tcacgcgcgg
     6601 tgcgggcttg agcgccaccg cgggcgcctt ggctttcccc cagccgccga atgccgccgt
     6661 gagcgcctgc ttcatccact tcggatccag atcgccggcg aacaccagcg tggcgcggtc
     6721 ggcgccaaaa tttccggcgt ggaagctcac gacgtcgccg tgcgtgatgg cggccagcgt
     6781 tcgttcgctg ccgccctgcg gacggccata ggggtggctg ccgaacaacg cggcgcgccc
     6841 gtagatgccg acgatctgcg agggatcgga atccttggcc gacttgatga actcgatatc
     6901 gcgatcacgg tagctcgaga attcctgcgc gtcgaaacgc ggacgcatca acgcgtcggc
     6961 caggagctcg atcatgagcg cgcggtcccg cgccaggaac tgcccgttga ccgagatcga
     7021 ctcggaaccg gcagtagcgt tgaagctgcc acccacgccg gcgacggcat cggcgaactc
     7081 gaacgcattg cgcttgccgg cgccgcgatc gagcaggccg gcgaccagcg aagacacacc
     7141 gggcttgtcg gcgggatccg cgagcacgcc gccgcggatg aagccgctga aggcgatcaa
     7201 cggtacgtct tttcgtggca ccagcacgat ggtcaggccg ttcggcaata cgaagcgctg
     7261 gttggcaggc accttgacgc caacggcggc ggtactgtcg gcgccgatcg ccgcgcccga
     7321 aaacgccagc gcgccaccca gcagcaacgc ggccacgcgg gcggcggcat ttttcagcat
     7381 gtggcgcttc atggcttcgc ctccagcact ccgaccgtcc ggtgattttt ctgcagcacc
     7441 agcgccgcgg ccttctgcac gtcctcgcgc gtgaccttgt cgtacgccgc gggcgaatcg
     7501 aacagcttcc tgtagtcgcc ctcgaacacg tcgaagctgc ctaacaacaa ggccttgcca
     7561 ttgatggtgg ctatctgctt ccagaacgcg gcagcataga ggttcttcgc acgcgcaagc
     7621 tcggcatcgg tgacgccctg cgccaccacc tggtcgagcg ccgcatccag caccttctcc
     7681 acttcggcga cgttggcgcc ctcgggcagc gtgagttgca cccatgacag gcccggatcg
     7741 aacccttcct ggaacgatcc gtccacttcg atggcgagcc tcttctcttc caccagcagc
     7801 cgatgcaggc gcgaagcatc tccttcggtg aggacggaca tgagcagatt gatggccggg
     7861 ccctgggggt cgttggccgc gggcgacttg taggcgtagt aaagcagcgg cgtcgacgct
     7921 tcgcgctgca cgaacacgcg cttctcgccg agctgttcgg gttcgacggt gcgcaccggc
     7981 ggcggcggct cctgccgggg gatcggttcg aggtatttct tcgccagggc gaaaccctct
     8041 tcggcgctga ggtcgcccac catgatcagc gtgcagttgt taggcgcgta attcgtcttg
     8101 aagaatttct gcaggtcttc gatcttccag ccctggatgt cggccggcca gccgacggtg
     8161 ggaaactggt acggatgcgc cacgaacgcc gtggcctgga cctgctcgac caggaatccc
     8221 gcgttgttgt cttcgacgga gagacgccgt tccgagtaga cgacgccacg ttcactctcg
     8281 accaccttcg ggtcgaagga aagattcgcg agccggtcgg attccagatc gaacaccagg
     8341 tcgagcgccg tgcgtgggat ccagtcctgg tagacggtga cgttctcaga ggtgaacgcg
     8401 ttgttggacc cgccgcgggc ctccatgatc cggtcgaatt cacccggcgt gcgcctggcg
     8461 gtgccgttga acatcatgtg ttcgaagaag tgcgcgaggc ccgtgatacc ggggacttca
     8521 ttgcgactgc cgactcgcac ccagttgtag agcgcaacgt tcgggatgtc gcgatcggtc
     8581 cagacgatga ctttcatgcc attggccagc gtggtcgcct gtacctggtc caggcctggc
     8641 acgcggactg gtgcggtggg tttggtggcg gcactgctcg aggcgctgaa gaacaagacg
     8701 caggcgcaaa gcgcgggaac gacgaaagaa ttcggcatgc ggcacacctc gacgaagagt
     8761 gcgcacttta tacggctcac ggaaaagaaa aagcgccgca aacgggggaa gtcgtttgcg
     8821 gcgccgaaga agcgaacacc ggggaagagt tgttcgcagt ccttgttgag gaccctatga
     8881 aatagggact acctcgaaac cttgacggcg tggatcgcag tgagctgggc cctcgcgagt
     8941 cgcgcgtcga tgggagcgaa catcacgctg ccaccgcggc gacgcttgac cagcattccc
     9001 gccagcgccg gccgcatgct cgcgacgccc tgcgcgtggg gaagaatcag cgttactgtg
     9061 gatctcgcca tcacggtgta ggacaaagca acgcccgtgc catccgggtg cccccaataa
     9121 aatcaataac ttgggatgag cgctcgtcct gacgcgccgg acgaagtgac gcccagcgtc
     9181 accttgtgac gcagttcatg cgcgcgcgag gttagaagct gccgtacttc tgttaacgag
     9241 gtcccactgc aggcagtaga tgttagggat gcgggataac cggcacgcaa ggcttcttcg
     9301 ccaccttgag accgccgcag atcgcacgtg cctgcacttc atcacgcact tcggcctgca
     9361 ggcgatagat cgcttttccc tgcgcctcgc caacgacgac gcgtggcgag agaccgtcga
     9421 gcagacccgc ggaatcagcc tgcagctttt tccattcttc gttggcgcgt tccgccgacg
     9481 agaacgcgcc gagctggact ccgtatccac tggccacagg tgtcgacgcc ggtgtgtcag
     9541 gcagcgatgc cccttcgccc gagggcgcgg ccactggtgg cgtcgcgccg gcggacgatc
     9601 ccgcgcctgc gacggctgcg gctgtgtcgg cagaagcgaa attatcgatg cgcacctgcg
     9661 cttcctttgc ccacttgccg gccggatatt gcgcgaggaa ttgctggtat gcctcgagcg
     9721 aatccgtctg tgccgcggcg cgccaatcct tttcctcggc gagctgctgc agtcgctcac
     9781 gcgctgtcgc gacgagcgtg ctgtcgggat gtcccgcgat gaactgctcg tacgattcgg
     9841 gtgaatccgc cgcctgcgcc gagcgccagt cgctcttctc gcggctgcag gcggcaagag
     9901 caagcaatgg gattgcgaac aacgtcacga gagcgcgggt ggacatggtt atgattccct
     9961 ggaacgaacg gtttattgta aacgcagccg ccgaccggag atgcacatga gcgaagaacc
    10021 gaagcgcaga ctcgtcgacc gattgggcat gtcacccact gtcatcgccg aaaacaccac
    10081 cgtggtgggc gacatcgaga cgcgcggccc gctcatggtc tccggcaatg tgcagggcaa
    10141 tggcacgatc ggcggcacgc tgagcgtggg caagagcgct cactgggtgg gtgacatttc
    10201 ggctcgccag gccgtgctcg ccggcaaggt caccggcagg atcgtggtag aagacaagct
    10261 cgagatcagc gccagcgcgg tgatctccgg ggagatcgtc gcgaagattc tcgccatcgc
    10321 caacggggcg cgcatcgaag gcgaagtcac cgtgaccagc gggaagccga tcctcaaatt
    10381 cgaagagaaa cgcgccgccg gctaattgca gttggcgagc ccgtcta
//
LOCUS       DASQJS010000319         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1132724, whole genome shotgun sequence.
ACCESSION   DASQJS010000319 DASQJS010000000
VERSION     DASQJS010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132724"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VEW08_12835"
     CDS             complement(<1..327)
                     /locus_tag="VEW08_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYJ41670.1"
                     /translation="MQRIRNFSIIAHVDHGKSTLADRFIQICGGLEAREMQDQVLDSL
                     ELERERGITIKAQAVTLYYKSKKDGETYQLNLIDTPGHVDFSYEVSRSLAACDGALLV
                     VDASQGV"
     gene            462..662
                     /locus_tag="VEW08_12840"
     CDS             462..662
                     /locus_tag="VEW08_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41671.1"
                     /translation="MANNAAIEQQYRHFEAELANELGVGIDIDHADGRQCLGAFQLGQ
                     LVQHLVTKPTTLAGDHYETRGD"
     gene            complement(693..1658)
                     /locus_tag="VEW08_12845"
     CDS             complement(693..1658)
                     /locus_tag="VEW08_12845"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HYJ41672.1"
                     /translation="MSTGVAFSAEPKEWLARMNKALTTQNYVGVFTHVHGGRVETLRI
                     IHRVRGGDVSERLLSLDGSGREFIREGNELVCYFPDRREVLVERRAPDGPLLGALPSI
                     DEDNSLVYEIRGGERQRLLGRDTRVVALQPRDQYRYGYRLWIDEKTSMPLKTQLCNQA
                     GEVIEQIVFSTIDLPERISDSMFKPQVDASTYRWFRADRRVAANSRPALWDAMRLPPG
                     FRIATRSSQSMPGSSEPVEHLVYTDGIASVSVFVEPRKPDSKPSEGPSRVGSSSAFST
                     VVDGHQVTAVGEVPPTTVELIAKQVKASKSPAPPAGAMGLAPQRK"
     gene            complement(1750..2478)
                     /locus_tag="VEW08_12850"
     CDS             complement(1750..2478)
                     /locus_tag="VEW08_12850"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HYJ41673.1"
                     /translation="MNEERDSQLSAMFDGELPGAECELLARRLTRDEALRAQWSRFSM
                     IGAALRSERGVALHDRVAWRVQSQVAQDPTYGDGSFDTATTSTHTAVVAPASVKATPR
                     WARFARPVAGASIAAGVAAMSIMWLRTDSGPDPMLASNPVTETIVLAPEATGTTVALN
                     ETPAAEPVPSNGEPDVYVTPVPSSSTSIAPPARLANYVVAHSEYSSPLSRRMALLGLV
                     ATDPESTAPAEPNADAAAGAADAP"
     gene            complement(2475..3083)
                     /gene="rpoE"
                     /locus_tag="VEW08_12855"
     CDS             complement(2475..3083)
                     /gene="rpoE"
                     /locus_tag="VEW08_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HYJ41674.1"
                     /translation="MTVEESDLVLVKRVQRGDKTAFDLLVRKYQHKVVKLVLRYVRNP
                     AEAEDIAQEAFIKAYRALPQFRGDSAFYTWMYRIAINTAKNSLASRDRSPIAYDLDLN
                     DPEESHSVQTKLQDPDTPEGMALTEEIRQIVNSAIEGLPEELKTAIVLRELDGLSYEE
                     IAAAMECPVGTVRSRIFRAREAIDKRLREVFEGGLGRTEEMV"
BASE COUNT          601 a          928 c         1035 g          581 t
ORIGIN      
        1 cacgccctgc gaggcatcga ccaccagcaa cgcgccgtcg caggccgcca gcgaacgcga
       61 cacttcatat gagaagtcca catggccggg ggtgtcgatg aggttcagct ggtaggtctc
      121 gccgtccttc ttcgacttgt aataaagagt gaccgcctgc gccttgatgg tgatgccgcg
      181 ttcacgctcg agctccagtg aatcgaggac ctggtcctgc atttcgcggg cttcgaggcc
      241 accgcagatc tggatgaagc ggtccgccag cgtggacttg ccgtgatcga cgtgcgcgat
      301 gatcgagaag ttgcgtatgc gttgcatcag ggaaggtcgg cctgccgccg atcttgcgga
      361 agagcgggat gctcaatcca aagcgccggc aaaaatcagc gcgggattat agcggcagga
      421 ggccgcagca gcctcaaaag ttcgttggaa ttcagcgtgt aatggcaaat aacgctgcca
      481 tcgagcagca gtaccggcac tttgaggccg aattggcgaa tgagctcggc gtcggaatcg
      541 acatcgacca cgctgacggg aggcagtgcc tgggtgcgtt ccagctcggc cagctcgtgc
      601 agcatctcgt cacaaagccc acaaccctcg cgggtgatca ctacgagacc cgcggtgact
      661 gaaggggcca ctgaagaggc cactgaaact gactatttgc gctggggcgc caaccccatg
      721 gctccagcgg gcggcgccgg ggatttggag gctttgacct gcttggcgat gagttcgacc
      781 gtggtgggcg gcacttcacc caccgccgtc acctggtggc catcgaccac cgtggagaaa
      841 gccgaagagg aacccacgcg cgaggggcct tcagatggct tgctgtcggg cttgcgcggc
      901 tcgacgaaca ccgacaccga ggcgatgccg tcggtgtaga cgagatgctc aaccggctcg
      961 ctggagccgg gcatggactg cgacgaacgc gtggcgatgc ggaagccggg aggcaggcgc
     1021 atggcgtccc acagtgcggg tctggaattg gcggctacgc ggcgatcggc gcgaaaccag
     1081 cggtaggtgg acgcgtccac ctgcggcttg aacatggagt cggaaatgcg ctcgggcaga
     1141 tcgatggtgg agaacacgat ctgctcaatg acttcgccag cctggttgca gagctgcgtc
     1201 ttgagcggca tcgaggtctt ttcgtcgatc cacaggcgat aaccgtagcg atattggtcg
     1261 cgcggctgaa gagcgaccac gcgggtgtcg cggccgagca ggcgctggcg ttcgccacca
     1321 cggatttcat agaccagcga attgtcttcg tcgatggaag gtaaagcacc gagcagcgga
     1381 ccgtcgggag cgcgacgctc gaccaggact tcgcgacgat cggggaaata acagacgagc
     1441 tcgttgcctt cgcgaatgaa ttcacggccc gagccatcga gcgacagcag ccgttcggac
     1501 acgtcgcccc cgcgcacgcg gtggatgatg cgcagggttt ccacgcggcc accgtgcacg
     1561 tgggtgaaca cgccgacgta gttttgcgtg gtgagcgcct tgttcatgcg cgccagccat
     1621 tccttgggtt cggcgctgaa tgcgacgccg gtgctcatca gcagcgtgaa agcggcgatc
     1681 cgagcgacgt tcttccgttg agccttcttc gtctgggaag gggcgcagct cgaattgcgg
     1741 agcttcaaat cacggagcat ctgcggctcc tgcggcggca tcggcattcg gttcggccgg
     1801 cgccgtggat tccggatcag tggctaccag gccaagcagc gccatgcggc gcgacaaggg
     1861 cgaagaatat tcgctgtgcg ccaccacgta atttgcgagg cgcgccggcg gcgcgatgct
     1921 ggtggaagaa ctcggaacag gagtgacgta gacgtccggt tcgccattgg aaggaacagg
     1981 ctcggccgcg ggcgtctcgt tgagcgcaac ggtggtaccc gtggcttcag gcgcgagaac
     2041 gatcgtttcg gttacggggt tgctggcgag catcgggtcc ggacccgagt ccgtgcgcag
     2101 ccacatgatg gacatggcgg cgacaccggc ggcaatgctg gcgccagcga ccggtcgagc
     2161 gaaccgggcc cagcgaggcg tcgctttgac agaggcggga gcgacaacgg cggtgtgcgt
     2221 ggatgtggtg gcagtgtcga aagatccgtc accgtaagtg gggtcctggg cgacctgcga
     2281 ctgaactcgc caggcgacgc ggtcgtgcag ggccacgccc cgctccgagc gcagagcggc
     2341 gccgatcatg gaaaaacgag accactgtgc ccgcagtgct tcatcgcgag tgagccggcg
     2401 tgcgagcaat tcgcattcgg cgcccggcaa ctcaccgtcg aacatcgcag acagctgact
     2461 gtcgcgttcc tcgttcatac catttcctct gtgcggccca ggccgccttc aaaaacttcg
     2521 cgcagccgtt tgtcgatggc ctcgcgcgct cgaaaaatgc gggagcggac cgtgcccacc
     2581 ggacactcca tggctgccgc gatctcttca taactcagcc catcgagctc gcgcagaacg
     2641 atggccgtct tgagttcttc aggcaatcct tcgatcgcgg agttcacgat ctggcgaatt
     2701 tcttcggtga gcgccatgcc ttccggcgtg tcggggtcct gaagcttggt ttgcacgctg
     2761 tgcgattcct cagggtcgtt cagatccagg tcgtatgcga tcggactgcg atcgcgcgac
     2821 gcgagactgt tcttggcggt gttgatagcg atgcggtaca tccacgtgta gaaggcgctg
     2881 tcgccacgga actggggtag agcccgatac gccttgatga aagcttcctg cgcaatgtct
     2941 tccgcttcag ccggattgcg aacgtatcgc aacaccagct tcaccacttt gtgctggtac
     3001 ttgcgcacca gcaggtcgaa cgcggttttg tcaccgcgct gaacgcgttt gacgagaacc
     3061 aggtcacttt cttcaaccgt cacaggagca ccccggctcc tttcgagctg aatagaccct
     3121 gaacgcccac gaaagtttcc ttttg
//
LOCUS       DASQJS010000320         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2702997, whole genome shotgun sequence.
ACCESSION   DASQJS010000320 DASQJS010000000
VERSION     DASQJS010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2702997"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VEW08_12860"
     CDS             <1..744
                     /locus_tag="VEW08_12860"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HYJ41675.1"
                     /translation="GLQWDPLPSVLPNGLSWWRTYVPNQSGNDVWAPDVKVYNGRTYM
                     YYSVSTFGSKVSLIGLISASSIAAGDWRDDGLVIRTTASNDYNAIDPDLTIDANGAPW
                     LSFGSWNTGIKLTRLGSNMKPTGTLYSIASRGGGIEAPVIVQRNGYYYLFVSTGTCCQ
                     GVNSTYQIRYGRSTSITGTYTDKSGVSMMSGGGTLLDGGNDRWKGPGGQDIAGTGVIV
                     RHAYDAQDNGNAKLLFSNLNWDSDGWPRY"
     gene            864..1049
                     /locus_tag="VEW08_12865"
     CDS             864..1049
                     /locus_tag="VEW08_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41676.1"
                     /translation="MESAQAAPGAVGKVVIAGCGTSGWVAAMALSSAQVAQVVNALPI
                     RGDFIKHYCDASEGAWG"
     gene            1082..2344
                     /locus_tag="VEW08_12870"
     CDS             1082..2344
                     /locus_tag="VEW08_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYJ41677.1"
                     /translation="MILFLQIAVILATCRLFGWMVRKWLRQPPVIGEMIAGVVLGPSL
                     LGLLAPEIQHALFPAESKPILYVVAQLGIGLYMFLVGLEFRSEDFRTHARGAVAVAIT
                     GIVVPFLIAIAGTPYLLTIPGLFAAKVSSFDATLFLGAAIAITAFPVLARIIHERGLG
                     DSLIATVSLSAAAIGDAIAWCVVAVVLASIGAGPQVAVLAIAGGAILSAALIFLGPRL
                     FAPLGRLAEQERDAGEGMSTTLLAVALVLFTVSAWIADVIGLHAVFGGFLIGTAMPRG
                     VFAERIKQRLEPFTLAFLLPLFFAYSGLNTQLTVVNTPGLFVIALGVLAASIFSKFIA
                     CWAAARLSGQDHRSALGIGALMNARGLTELIILNIGLAAGIIGTALFSMLVLMAIVTT
                     LMASPLFEAVYGRAARSRGELPPLSSLR"
     gene            2405..2644
                     /locus_tag="VEW08_12875"
     CDS             2405..2644
                     /locus_tag="VEW08_12875"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYJ41678.1"
                     /translation="MATCRVIGWLGQKYLKQPQVVCEMIAGVLLGPSLFGLVAPDIQK
                     LVFTPESLGVLCVGAQFGVGLYMFLVGFGFRTDHF"
     gene            2911..>3553
                     /locus_tag="VEW08_12880"
     CDS             2911..>3553
                     /locus_tag="VEW08_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41679.1"
                     /translation="MPAAHAEELVAKQFTLRGFGTAAATTQNAEGLEFRRSTAQQLGV
                     EANEIELYTDSIAGVQVNAKLGSKFDVVLQGLTRQHADGGWNPEMSLGFLRFSPDESW
                     VLRAGRVGYDIYLLAESRQVGYSYLALRPAPELYGMMSSDGIDGGDVAYTHRLGRGLA
                     RARLFGGKGSGETAFADRSHVESDADVYGACFDYLYRGWTARLALVQYSTDAGP"
BASE COUNT          585 a         1119 c         1134 g          715 t
ORIGIN      
        1 ggactgcaat gggatccgtt gccctcggtg ttgccgaacg gtttgagctg gtggagaacc
       61 tacgtgccga atcagtcggg caacgatgtg tgggcgccgg acgtgaaggt ctacaacggc
      121 cgcacctaca tgtactactc ggtgtccacg ttcggctcaa aggtgtcgct gatcggattg
      181 atctcggcct ccagcatcgc ggccggtgac tggcgcgatg acggcctcgt catccgcacc
      241 acggcgtcga atgactacaa cgccatcgac ccggatctca ccatcgacgc caacggggcg
      301 ccgtggctgt cgttcggctc gtggaacaca ggcatcaagc tcacgcggct cggcagcaac
      361 atgaagccca cgggcacgtt gtattcgatc gccagccgcg gcggcggcat cgaggcgccg
      421 gtcatcgtcc agcgcaacgg ttattactac ctgttcgtct cgacgggtac gtgctgccag
      481 ggtgtgaaca gcacctacca gatccgctat gggcgctcga ccagcatcac cggcacttac
      541 accgacaaga gcggcgtcag catgatgagc ggcggcggca cgctgctcga cggtggcaac
      601 gatcgctgga aagggccggg cggacaagac atcgccggca ccggtgtcat cgtgcgccac
      661 gcctacgacg cgcaggacaa cggcaacgcg aagttgctgt tcagcaatct caactgggat
      721 tccgacggct ggccgcgtta ttgatcacgg tgccgggccg ggaaaccggg aaaactttcc
      781 gaacgaatgg agcgctagtc cagcgaaagt tttcccggtt tcccggcccg gcacctagaa
      841 tcccctcgag gaagggggtt cggatggaaa gcgcgcaggc cgcacccggt gcggtcggga
      901 aagttgttat tgccggctgc ggcacctcgg gatgggtggc cgccatggcg ctctcgagcg
      961 cgcaggtggc acaggtggtg aacgccctgc cgatacgcgg cgacttcatc aagcactact
     1021 gcgacgccag cgaaggggcg tggggctgag ccgccggact cggggcagcc gccgctaacc
     1081 gatgattctc tttcttcaga tcgccgtcat tctggcgact tgccgcctgt tcggctggat
     1141 ggtgcgcaag tggctgcgcc agccaccggt catcggcgag atgatcgcag gcgtggtgct
     1201 cggaccatcg ttgttggggc tgctggcgcc ggaaattcag cacgcgcttt ttccggccga
     1261 gtcgaagccc atcctgtacg tagtggcgca gctcggtatc ggcctgtaca tgttcctggt
     1321 cgggctcgag tttcgcagcg aagacttccg cacgcatgcg cgcggcgcgg tggccgtggc
     1381 gatcaccgga atcgtcgtgc cattcctcat cgccatcgcc ggcacgccgt atctgctgac
     1441 catacccgga ctcttcgcgg cgaaagtgtc gtcgttcgat gccacgttgt ttctcggcgc
     1501 ggctattgcc atcaccgcgt ttccggttct cgcgcgcatc atccacgagc gcggcctcgg
     1561 cgattcgctg atcgccactg tttcgttgtc ggccgcggcc atcggcgacg ccatcgcgtg
     1621 gtgcgtggtg gcggtggtcc tcgcaagtat cggtgctggt ccgcaggtgg cggtgctggc
     1681 gatcgcaggt ggagccattc tttcggcggc cctgattttt ctcggaccgc ggctgttcgc
     1741 accactcggc cggctagcgg aacaggaacg agatgcaggc gagggcatga gcacgacact
     1801 gcttgcggtc gcgctggtgc tgttcaccgt ttcggcatgg attgccgatg tcatcggatt
     1861 gcacgccgtg tttggtggct tcctgatcgg caccgccatg ccgcgtggag ttttcgccga
     1921 gcgcatcaag cagcgtctcg agccgttcac gctggcattc ctgctgccac tgttcttcgc
     1981 gtactccggc ctcaacacgc aactgactgt ggtcaataca ccaggattgt tcgtcattgc
     2041 gctcggcgtg ctcgcggcgt cgattttttc caagttcatc gcgtgctggg cggcggcgcg
     2101 actctccggc caggaccatc gcagcgccct cggaatcgga gcgctcatga acgcccgcgg
     2161 tctcaccgaa ctcatcattc tcaatatcgg actggcggcc ggcatcattg gtaccgcgct
     2221 gttctccatg ctggtgctca tggcaattgt cacgacactc atggcatcgc cccttttcga
     2281 agcggtttat ggccgcgccg cgcggagccg gggcgagttg ccgccgctga gcagtcttcg
     2341 ataggcagaa tttatcggcc acccgctggg cggccgcggc tctccgcccg tataaaagcc
     2401 catcctggct acctgccgtg tcattggttg gctcggccag aagtacctga agcagccgca
     2461 ggtggtctgc gaaatgatcg ccggtgtgtt gttaggccct tcgctgtttg gcctggtggc
     2521 gcccgacatc cagaaactgg tgttcacgcc cgagagcttg ggcgtgctct gcgtcggtgc
     2581 gcaattcggt gtcggcctct acatgttcct ggtggggttc gggttccgca ccgatcactt
     2641 ctaagccaac gtccacacgg cgcctttctg cgtcgcgatc ggccccccgc tgactcgagc
     2701 gatcagtcgc gacccaccta ctctttaccc tgtaggacac gcacctgctg ggcgcacgca
     2761 cttcgggaat ctgcgcattg gctccccttt cccgcaacac agtttcgcag atgagacccc
     2821 tggcactcat gtattacgcc gcacggccgc taacggagca tgaaccgaaa gaccctggga
     2881 ataagcgctt ccgttgttct cttccttgcc atgcctgcgg cgcacgccga ggaactcgtc
     2941 gcgaagcaat tcacgctgcg cgggttcggt actgccgccg caaccacgca aaacgcggag
     3001 ggcctcgagt ttcgccgcag caccgcccag cagcttggcg tcgaggccaa tgaaatcgag
     3061 ctgtataccg attcgatcgc gggcgtgcag gtcaacgcca aactgggctc gaagttcgac
     3121 gtcgtgttgc agggtttgac ccggcagcac gccgacggcg gctggaaccc ggagatgtcg
     3181 ctggggttcc tgcgtttctc tcccgacgag tcctgggtgt tgcgcgccgg acgagtgggt
     3241 tatgacatct atctactcgc ggaatcacgg caggtcggct attcctacct ggccttgcgc
     3301 ccggcgcccg agctgtacgg catgatgagc agtgacggaa tcgacggcgg agacgtcgcg
     3361 tatacgcacc gcctggggcg tgggctggcg cgcgcccgcc tgtttggcgg caagggttcc
     3421 ggcgagacgg cattcgccga tcggagccat gtcgagagcg acgccgacgt gtacggcgcc
     3481 tgtttcgact acctgtatcg cggctggacc gcgcgattgg cgctggtgca atacagcacc
     3541 gatgcaggtc ctg
//
LOCUS       DASQJS010000321         6873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2703813, whole genome shotgun sequence.
ACCESSION   DASQJS010000321 DASQJS010000000
VERSION     DASQJS010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2703813"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..542)
                     /gene="nusB"
                     /locus_tag="VEW08_12885"
     CDS             complement(99..542)
                     /gene="nusB"
                     /locus_tag="VEW08_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HYJ41680.1"
                     /translation="MAKGTGINPASARRAVARKLAMQALYRWQLNAAPWQDVLNEFAT
                     DEDMRKADRGYFNQLVTDVCGGSEALDAALAGWMDRKPTELDPVERAVLWVGAHELRS
                     APDVPYRVVINEAVGLAKRFGATDSHKFVNAVLDAAAKELRPNER"
     gene            complement(542..1072)
                     /gene="ribH"
                     /locus_tag="VEW08_12890"
     CDS             complement(542..1072)
                     /gene="ribH"
                     /locus_tag="VEW08_12890"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005173754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HYJ41681.1"
                     /translation="MTGSLSPAQAFDASRRRVVIIAARFNAPLVDQLIDGATRAWVAH
                     GGDASRLLVERVPGAFELPLAAKMFAASGAVEAVVALGCVIRGDTPHFDFVAGECARG
                     LMDASLATGVPVIFGVLTTETQAQAEERADLNRMNKGRESMEAALEMIQLLAPPPRVP
                     LASRGKSRPKSSKAKR"
     gene            complement(1098..2207)
                     /gene="ribBA"
                     /locus_tag="VEW08_12895"
     CDS             complement(1098..2207)
                     /gene="ribBA"
                     /locus_tag="VEW08_12895"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HYJ41682.1"
                     /translation="MKPLNSIEEILADIAAGRMVVIMDDEDRENEGDLVMAAQKVRAD
                     DINFMARFGRGLICLTLTAERCRQLRLPLMVSDTHKEHRTNFTLSIEAAEGVTTGISA
                     HDRAQTVIAAVKPNARPEDLRQPGHIFPVMAQPGGVLTRAGHTEAGCDLARLAGLDPS
                     AVIVEIMNDDGTMARRPDLESFAKQHGIKIGTIADLIRYRLKQERSVERIAERKVATE
                     FGEFTLLAYEDHVHRDVHLALVRGTLDASKPPLVRVHPIDTLSDLVGVRDPGRTWTLR
                     DAMQRIAREGVGIVIVLRDHSTPRELADAVSALGQRAGHPAPAHAAPQVLRTYGIGAQ
                     ILKDLGVTRMQVLSTPKQLQGISAFGLEITGYVAD"
     gene            complement(2204..2815)
                     /locus_tag="VEW08_12900"
     CDS             complement(2204..2815)
                     /locus_tag="VEW08_12900"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HYJ41683.1"
                     /translation="MFTGIIQEVGVIQRIETRTGAGAAEDRRIEVSFGSIARERLNPG
                     DSICVDGVCLTVAALGVSSFHADVSGETLRVTTMGAKAAGARVNLEPALRAGDSLGGH
                     WVSGHVDGVADVLATAGDARSLRVEFAAPRPLARYIARKGSVTLDGVSLTVNEVDGVK
                     FAVNLIPHTLEVTTLGTLAAGARVNLEIDLLARYVERLSLDTK"
     gene            complement(2816..3925)
                     /gene="ribD"
                     /locus_tag="VEW08_12905"
     CDS             complement(2816..3925)
                     /gene="ribD"
                     /locus_tag="VEW08_12905"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HYJ41684.1"
                     /translation="MSFSAFDEAAMRRALELAERGLYSTQPNPRVGAVLARDEEIVGE
                     GWHERAGEAHAEPIAIRAAGERSRGATAYVTLEPCNHHGRTPPCVDVLLAAGVRRVVY
                     AVGDPNPRVNGAGAARLRKAGVIVESGLLAAQSEALNAGFLMRMRHGRPFIRLKSGAS
                     LDGRVALANGRSKWITSEEARADVQHWRAQSGAVLTSAATVLADDPRLDVRIDAPLQP
                     LRVVLDRRRALRKTARILAPPGDVVVFAAPTATRKGGTGDERLGDARVERMRVKRSHL
                     DLTAVFARLAELEVNDVLVEAGPRLSGALLAAGLVDEWLLYVAPKLLGQGAKPVAFLG
                     RLTKLEAAPEFVMIDSKSVGPDLRLRLQPKLKDKQ"
     gene            complement(4015..4455)
                     /gene="nrdR"
                     /locus_tag="VEW08_12910"
                     /pseudo
     CDS             complement(4015..4455)
                     /gene="nrdR"
                     /locus_tag="VEW08_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006878018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
     gene            complement(4476..5732)
                     /gene="glyA"
                     /locus_tag="VEW08_12915"
     CDS             complement(4476..5732)
                     /gene="glyA"
                     /locus_tag="VEW08_12915"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HYJ41685.1"
                     /translation="MFRTAMTIGGFDPELDAALKNERQRQEDHIELIASENYVSPRVL
                     EAQGTVLTNKYAEGYPGKRYYGGCEFVDIAEQLAIDRAKKLFGADYANVQPHSGSQAN
                     AAAYLALINPGDTILGMSLDHGGHLTHGAKVNFSGKLFRAVQYGIRPDNGEIDYDALA
                     AQALAEKPRMIIAGFSAYSRYLDFKKFREIADSVGAYLFVDMAHVAGLVAAGLYPDPI
                     PYADVVTTTTHKTLRGPRGGLILARANEAITKKLNSLVFPGTQGGPLMHVIAAKAVAF
                     LEALQPEFKEYQKQVVANARATAVRLQKRNYKIVSGGTDNHLFLLDLSDKAINGKEAD
                     AALGKAHITVNKNAVPNDPRPPMVTSGIRIGSPAGTTRGFKEREFEQIADLVADVLDA
                     NGSDAAIEKARVSAGELCRRYPVYGP"
     gene            complement(5871..>6873)
                     /locus_tag="VEW08_12920"
     CDS             complement(5871..>6873)
                     /locus_tag="VEW08_12920"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41686.1"
                     /translation="YQGERRLSTAASSVRAPSVLLIAGERLARYGFGDGHPFGPDRHA
                     AFLAELVARGLDKRVQIMETRAATREELQSFHTPQYLDLVAERSRTGDGYLDGGDTPA
                     WRGVYEAAADVAGGTLLAAEQIMRGAARRAFIPIAGLHHAARGGAAGFCVFNDCGVAI
                     EMLRRKGLERIAYVDIDAHHGDGVYYGFEDDPNVIFADIHEDGRHLYPGTGSKDEIGR
                     GAAAGTKLNIPVPPGSDNSVFAAEWPRVIAHLRKFEPEFILLQAGADSVEGDPITHMR
                     FTADAHGRAARDLAALAEELGHGRVLGTGGGGYNRINLAQAWTAVVQGFVDAPVGAG"
BASE COUNT         1128 a         2282 c         2231 g         1232 t
ORIGIN      
        1 gtacttgcgg atgagctcga attcaccttg tggcatgggg gttcccaggc gctgcgcgcc
       61 atgtcggaaa acggcgccga ggcggcgccg tacatcaatc aacgttcgtt ggggcgcagc
      121 tctttggcgg ccgcgtcgag cactgcgttg acgaacttgt ggctatcggt ggcgccaaaa
      181 cgcttggcca ggccgacggc ctcgttgatg accacgcggt acggcacatc gggcgccgag
      241 cgcagctcgt gtgcgccaac ccacaacacc gcgcgttcca ccggatcgag ttccgtgggc
      301 ttgcgatcca tccagccggc gagcgccgca tcgagcgctt cgctgccgcc gcacacatcg
      361 gtgacgagct gattgaaata accgcgatcg gccttgcgca tgtcctcgtc ggtggcgaac
      421 tcgttgagta cgtcctgcca cggagccgcg ttgagctgcc agcgatacag cgcctgcatg
      481 gcgagcttgc gcgccacggc gcggcgcgcc gaggccgggt tgatgccagt gcccttcgcc
      541 atcagcgttt cgccttgctc gacttcgggc gcgatttccc gcgcgacgcc agcgggaccc
      601 gcggcggcgg cgcgagtagc tggatcattt cgagcgcggc ctccatcgat tcacgtccct
      661 tgttcatgcg attgaggtcc gcgcgttcct cggcctgcgc ctgtgtctcg gtggtgagca
      721 cgccaaatat caccggcaca cccgtcgcga ggctggcatc catcaggcca cgcgcacatt
      781 cgccggccac aaaatcgaaa tgcggcgtgt cgccgcggat cacgcaaccg agtgcgacca
      841 ccgcttccac cgcaccactg gcggcgaaca tcttggcggc cagcggcaat tcgaaggcgc
      901 cgggaacccg ttcaaccagc aggcgcgatg catctccgcc atgcgcgacc caggcccgcg
      961 tggcgccgtc gatgagctga tcgaccagcg gcgcgttgaa acgggcagcg atgatcacga
     1021 ctcggcggcg gctggcgtcg aaggcttgtg ctggggagag ggatccggtc atgggcgcgg
     1081 gagtatatag gccgccgcta atcggcgacg tatccggtga tctcgagccc gaaggccgag
     1141 atgccctgca attgcttggg cgtcgagagc acctgcatgc gtgtgacacc caggtccttg
     1201 agaatctgcg caccgatgcc gtaagtccgc agcacctggg gcgccgcgtg ggctggcgcg
     1261 ggatgcccgg cacgctgacc cagcgcactc accgcgtcgg ccagttcgcg cggcgtgctg
     1321 tgatcgcgca gcacgatgac gatcccgacg ccctccctgg caatgcgttg catcgcgtcg
     1381 cgcaaagtcc aggtgcggcc cgggtcgcgc acgcccacga ggtccgacag cgtgtcgatg
     1441 ggatgtacgc gcaccagcgg tggcttcgac gcatcgagcg tgccgcgcac cagggccagg
     1501 tgcacgtcgc gatgcacgtg atcttcgtag gctaacaacg tgaactcacc gaactcggtc
     1561 gccaccttcc gctcggcaat gcgctccacc gaacgctcct gcttcagccg gtagcggatc
     1621 aggtcggcaa tcgtgccgat cttgatgccg tgttgttttg cgaagctctc caggtccggc
     1681 cggcgagcca tggttccgtc gtcgttcatg atctcgacga tgaccgccga tggatcgagc
     1741 ccggcgagcc gcgcgagatc gcatcccgcc tcggtgtgac cggcgcgcgt cagtacaccg
     1801 cccggctgcg ccatgaccgg gaagatgtgt cccggctggc gcagatcttc ggggcgcgca
     1861 ttcggcttta ccgcggcgat gaccgtttgc gcacggtcgt gggccgagat gccggtggtg
     1921 acgccttcgg cggcctcgat ggacagcgtg aagttggtgc ggtgttcctt gtgcgtatcg
     1981 ctgaccatga gcggcagccg cagctggcgg catcgttcgg cggtcagcgt caggcagatg
     2041 aggccgcggc cgaagcgcgc catgaagttg atgtcgtcgg cccgcacttt ctgggcggcc
     2101 atcaccagat cgccttcgtt ctcgcgatcc tcgtcatcca tgatcaccac catgcgcccg
     2161 gcggcgatgt cggcgagaat ttcttcgatg ctgttgagcg gtttcatttg gtgtccagtg
     2221 agagccgctc tacgtagcgt gccagcaggt cgatttcgag attcacgcgc gcacccgcgg
     2281 cgagcgtgcc gagcgtggtg acttcgagcg tgtgcgggat gaggttgacg gcgaatttta
     2341 cgccgtccac ttcgttgacc gtgaggctca cgccatcgag cgtgaccgaa cccttgcgcg
     2401 cgatgtagcg cgccagcggc cggggcgccg cgaattccac acgcagcgaa cgcgcgtcgc
     2461 ctgcagtcgc cagtacatcc gcaacgccat cgacgtggcc ggacacccag tgaccgccaa
     2521 ggctgtcgcc cgcgcgcagg gccggctcga gattgacacg cgcgccagcg gccttggcgc
     2581 ccatcgtggt gacgcgcaac gtttcgcccg aaacatcggc atgaaagctg gagacaccga
     2641 gcgccgccac ggtcaggcac accccatcca cgcaaatgct gtcacccgga ttcagccgct
     2701 cacgcgcgat ggagccgaat gacacctcga tgcggcgatc ttcggcggcg cccgctcccg
     2761 tgcgagtttc gatgcgctgg atgacgccga cttcctggat gatccccgtg aacatctatt
     2821 gtttgtcctt gagcttcggc tgcaaccgca ggcgcaaatc cgggcctacg gacttcgaat
     2881 cgatcatcac gaactcgggt gccgcctcga gctttgtgag tcgcccgaga aacgctacag
     2941 gtttcgcacc ctggcccaat agtttcggcg cgacatacag cagccattcg tcgacgagac
     3001 cggcagcgag cagcgctccc gacagccggg gccccgcctc caccagcacg tcgttgacct
     3061 cgagctccgc cagccgcgcg aagaccgccg tgaggtcgag gtgcgagcgc ttcacgcgca
     3121 tccgctcgac gcgcgcatca cccaggcgtt cgtcgcccgt tccgcctttt cgcgtcgccg
     3181 tcggtgcggc aaaaaccacc acatcgccgg gcggcgcgag aatgcgcgcc gtcttgcgca
     3241 gcgcacgacg ccgatcgagc acgacgcgca gcggctggag cggtgcgtcg atgcgtacgt
     3301 cgaggcgcgg atcgtcggcc agcacggtgg cggcgctggt gagcaccgcg ccgctttgtg
     3361 cacgccagtg ctgaacatcc gcgcgcgcct cctcgctcgt gatccacttg ctcctgccgt
     3421 tcgccagcgc aacgcggccg tcgaggctgg caccggactt caggcggatg aatggccgcc
     3481 cgtgacgcat gcgcatcagg aaccctgcat tcagcgcttc tgattgcgcg gctaacaacc
     3541 ccgactcgac gatcacgccc gccttcctga gccgcgccgc accggcgccg ttgacgcgcg
     3601 gattcggatc gccgaccgca tacaccacgc gccgaacgcc cgcggccaac agcacgtcga
     3661 cacacggggg cgtgcgaccg tgatggttgc agggctcgag cgtcacatag gcagtggcgc
     3721 cgcgtgagcg ttcgccggca gcgcggatcg cgatgggctc ggcgtgcgcc tcgcccgcgc
     3781 gctcgtgcca gccttcaccg acgatttcct catctcgcgc cagcaccgcg ccgacgcgcg
     3841 gattgggctg agtgctgtac aggccacgtt ccgcgagctc gagcgcgcgg cgcatggcgg
     3901 cttcatcgaa tgcgctgaaa ctcatttgcg ctccttgccg gctttctttg gcgagacgtc
     3961 gccgggcaac agcgacagct ggccttcact tggaaccttg ccgcgccggt gcctcagccg
     4021 gtcaacctct tcgcgaaacg cttcgacgtc ctggaagtgg cggtataccg aggcgtaacg
     4081 cacataggcc acttcatcga gcttgtgcag ttcttccatg acgatttcgc cgatctgccg
     4141 ggacgtgact tcgcgctcgc cgagactgcg cagcttgcgc gtgatgtgtc ccacggcgtc
     4201 ttccaccgat tcacggctta ccgggcgctt ctccagcgcc ttgtgcagtc caccgcgcag
     4261 cttgttctca tcgaaaggaa cgcggcgctg atcgttcttc accaccagcg gcatcaccag
     4321 ctcagcggtt tcgaacgtgg tgaaacgctc gccgcaggcc aggcactcgc ggcgccggcg
     4381 aatctgcgtg ccctcggccg ccagccggga gtcgtttacc ttggtgtcgt cgtgcgcgca
     4441 gaagggacag tgcatggcac gccctcgtta acggcttacg gcccgtaaac cggatagcga
     4501 cggcagagct cgccggcgga aacacgcgct ttttcgatgg cagcgtcgct gccgttcgca
     4561 tcgagaacgt cggccacgag atccgcgatc tgttcgaatt cgcgctcctt gaaaccgcgc
     4621 gtggtgccgg cgggcgagcc gatgcgaatg ccgctggtca ccatcggcgg gcgcgggtcg
     4681 tttggcaccg cgttcttgtt gacggtgatg tgcgccttgc caagcgcagc atccgcttcc
     4741 ttgccattga tcgccttgtc ggacaggtcg agcaggaaca ggtgattgtc ggtgccgccg
     4801 gagacgatct tgtagttgcg cttctggagg cgcacggccg tcgcgcgcgc gttggccacg
     4861 acctgtttct ggtattcctt gaactcgggc tgcagcgctt cgagaaacgc cacggccttc
     4921 gcggcgatga cgtgcatcag cggaccgccc tgcgtgccgg ggaacaccag cgagttgagt
     4981 ttcttcgtga tggcctcgtt ggcgcgcgcc aggatcaggc caccgcgcgg accgcgcagc
     5041 gtcttgtgcg tcgtcgtcgt gacgacatcg gcgtacggaa tcggatccgg atacaggccc
     5101 gcggccacga ggcccgcgac gtgcgccata tcgacgaaca gataagcgcc gaccgaatcg
     5161 gcaatctcgc ggaatttctt gaaatcgagg taacgcgaat aggcggagaa gcccgcaatg
     5221 atcatcctgg gtttttcggc cagtgcctga gccgcgagcg cgtcgtagtc gatctcgccg
     5281 ttgtccggac gaatgccgta ctgcacggcc cggaaaagtt ttccggagaa gttcaccttg
     5341 gcgccgtgcg tgagatggcc gccgtgatca aggctcatgc cgaggatggt gtcgcccggg
     5401 ttgatcaacg cgaggtacgc cgcggcgttg gcctgcgaac cggaatgcgg ttgcacgttg
     5461 gcgtagtcgg cgccgaagag cttcttggcg cggtcgatgg cgagctgctc ggcgatatcc
     5521 acgaactcgc aaccgccgta gtagcgcttg cccgggtagc cctcggcata tttgttggtg
     5581 agtaccgtgc cctgagcttc cagcacgcgc gggctgacgt agttttccga ggcgatcagc
     5641 tcgatgtgat cttcctggcg ctggcgttcg ttcttgaggg cggcatcgag ttcggggtcg
     5701 aaaccaccga tggtcatcgc ggtacggaac atggaaaagg cctcacgggt cgacttccgg
     5761 gggccgcgaa ttgtactgga tgtgcccagt tggtgccggg tgaggaaagt ggggagtcgc
     5821 tcgccgatct gcgacctacc aatcccagcg ttggcgactc cccactttcc tcacccggca
     5881 cccaccggag catcgacgaa tccctgcacg accgccgtcc aggcctgggc gagattgatg
     5941 cggttatagc cgccgccccc ggttccgagc actcgaccgt gtcccagctc ttccgccagc
     6001 gctgccaggt cccgcgccgc ccggccatgc gcgtcggcag tgaatcgcat gtgggtgatg
     6061 gggtcgcctt cgacgctgtc ggctccggcc tggagcagga tgaactcggg ctcgaacttg
     6121 cgcagatgcg cgatcacgcg cggccactcg gcggcaaaga ctgaattgtc cgaacccggt
     6181 ggtacaggaa tgttgagctt ggtgcccgcg gccgcgccgc gcccgatttc gtctttgcta
     6241 cccgtgccgg ggtacaggtg acggccgtct tcgtggatgt cggcgaagat cacattcggg
     6301 tcgtcctcga agccgtagta cacaccgtcg ccgtggtgag cgtcgatatc cacgtaagca
     6361 atgcgttcaa gacccttccg gcgcagcatt tctatagcca cgccgcagtc gttgaaaacg
     6421 cagaaccccg cggccccacc tcgcgccgcg tgatgcaacc cggcgatggg aatgaacgcg
     6481 cgccgcgccg cgccacgcat gatctgctcg gcggcgagca aagtgccgcc cgcgacatcc
     6541 gcggcggctt catagacgcc gcgccaggcc ggcgtatcgc ccccgtcgag gtaaccgtca
     6601 ccagtgcgcg agcgttcggc gaccaggtcg aggtactgcg gggtatgaaa ggattgcaat
     6661 tcctcgcgcg tggccgcgcg cgtctccatg atctgcacgc gcttgtccag ccctcgtgcg
     6721 accagctcgg cgagaaaagc ggcgtgacgg tccggaccga aagggtggcc gtcgccaaag
     6781 ccataccgcg ccaggcgctc gcccgcgatc agtaaaaccg atggcgcccg cacagatgag
     6841 gctgcagtgg acagtcttct ctccccttgg tac
//
LOCUS       DASQJS010000322         4947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2704965, whole genome shotgun sequence.
ACCESSION   DASQJS010000322 DASQJS010000000
VERSION     DASQJS010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2704965"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2452)
                     /locus_tag="VEW08_12925"
     CDS             complement(<1..2452)
                     /locus_tag="VEW08_12925"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYJ41687.1"
                     /translation="MAGDSKVSATLEKLIFGNRVAIVAAFVLLTVGLAAVAVRGLHID
                     ASFTKQLPLKHEYMQTFVKHQAEFGGANRVLIALVARDGNMFTPGFFDALKKATDEVL
                     VMDGIDRSRVQSLFTPNVRYMEVVEEGIEAGNVVDSEFVPTPEALAKVRENILKAGIR
                     GRLVANDFSGALVSAIVLEKDATGKPIDPIEVARDLESRVRDKIQNNDVLVQAQGGGI
                     DVHMIGFAKVVGDIADGAMSVIVFAIVTILLTLLAVWYYVQSLKVAFVPIVCSIVAVT
                     WQLGTLVLLGYGIDPLGLLVPFLIFAIGVSHGVQKISAVKEAAVAGLSSMDAARQTFR
                     QLLMPAIIALLADLVGFITILLIPVQVVQEMAVTASIGVAIVILTDLILLPVMVSWVR
                     WDDKYKQRVELRQAKLTVHWARLARITERKPAAVIIGIAVLLGIFGVWKGRETPIGDT
                     QSGVPELRADSRYNRDSNIITSKFSIGVDILTVIVETKEPACTSHELMTAIDTFAWKM
                     RNVDGVQEVMALPIVAKIAIAGWNEGSLKWRNIPREPNQLTQSTRYIETSTGLLNADC
                     NVIPVMLFLRDHKAATIERVVSAVKEWRTLNPMPGATFNLATGNVGVMAATNEEVEAK
                     EYPILGWVFAAVILMCLLTFRSLLGTVLVFLPLALVSILVYAVMAIVGIGLKVNTLPM
                     VALGAGIGVDYGIYLWSRMQEYLHAGDTVHQAFEKTMRVTGASIIFTGITLAIGVVTW
                     VFSPLQFQADIGIMLTFMFFVNMLGAILLLPALASLFVRARKPPDRRDESADHDNYAG
                     GGRRDDSTAHDGTSTDADR"
     gene            complement(2454..3464)
                     /locus_tag="VEW08_12930"
     CDS             complement(2454..3464)
                     /locus_tag="VEW08_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41688.1"
                     /translation="MRHRMSLALVATLAVAGIAPGITQAQLDPNDGANRPAEIEPLAS
                     SSLLLDLAVAGQRLVAVGERGHVMLSDDHGATWRQAKSVPTRVLLTAVFFVDAEYGWA
                     VGHDETILNTADGGENWTRSHFAPEAQQPLLDLWFANRVSGIAVGAYGAYFTTNDGGR
                     HWSSARFSAPPTQPANEDEPPPDYHLNRIVGVGNRLYVAAEGGKLYRSDDRGASWRAL
                     PSPYQGSFFGLVPIRGEGLLAFGLRGHLFRSADAGETWHELESHTTAMLTDGIAINDL
                     RVVIGGLAGVLLVSADAGETFKLTQQDDRKGMSALLPGPAGTVIVAGEGGVRTIRVGE
                     GS"
     gene            complement(3559..>4947)
                     /locus_tag="VEW08_12935"
     CDS             complement(3559..>4947)
                     /locus_tag="VEW08_12935"
                     /inference="COORDINATES: protein motif:HMM:NF018716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HYJ41689.1"
                     /translation="ISMLKKHVLAMVATIAGIAIGASSAVAAVSAADAAKLGAELTPL
                     GGVKAGNADGTIPAWDGGLTSAAQAGFANFRPGQHHPDPYANDKPVYTVTSANMAQYA
                     GKLTEGHKKMFQTYGSTFKMNVYPTHRSAAAPQRIYDATKRIATTAQLAKGGNGVTNA
                     GEGIPFAIPKEGVEVFWNHVLRYRGDVIVRQIGQAPVTASGAFTMVKFRDDTMAAYSL
                     PGAKSENLDNTIVYFIQETIAPARLAGEVLLVQETLDQSLENRRAWVYNAGQRRVRRA
                     PNVAFDNPGTNSDALRTSDQFDLYNGSPQRYDWTLVGKKEMLVPYNAYKLNLNTLKYT
                     DILKKNHINPDVARYELHRVWVVDSKLKAGQSHLYSRRTLYVDEDSWHIVAVDCYDSR
                     GQLYRVQEGHVMQYYDVPALWTALETVYDLSNGRYLALGLDNEEPRSRDFSQKRTIAD
                     YQPAALQRRGVR"
BASE COUNT          852 a         1571 c         1611 g          913 t
ORIGIN      
        1 atctgtctgc gtccgtcgac gtcccgtcgt gagcggtcga atcatcgcgt cgtccgccac
       61 ccgcgtaatt gtcgtggtcg gcactctcgt cgcgacgatc tggcggcttt ctcgcccgca
      121 cgaacaggga tgcgagcgca ggtagcaaca ggatggcccc cagcatgttc acgaagaaca
      181 tgaaggtcag catgatgccg atatccgcct ggaactgcag cggcgagaac acccaggtga
      241 ccacgccgat ggcgagcgtg atgccggtga agatgatgct cgcgcctgtc acgcgcatgg
      301 tcttctcgaa cgcctggtgc acggtgtctc cggcgtgcag gtattcctgc atgcggctcc
      361 acaggtagat gccgtagtcc acgccgatgc cagcgccgag cgccaccatg ggcagcgtgt
      421 tgaccttgag gccgatgccg acgatggcca ttaccgcgta caccaggatg gagacgagcg
      481 cgagcggcag gaacaccagc accgtgccca gcaagctgcg gaacgtgagc aggcacatga
      541 ggatgaccgc ggcgaagacc cagccgagga tgggatattc cttggcttcc acttcctcgt
      601 tggtggcagc catgacgccg acgttacccg tggcgagatt gaaggttgcg cccggcatgg
      661 ggttgagcgt gcgccactcc ttgacggcgc tcaccacgcg ctcgatggtc gcggccttgt
      721 gatcgcgcag gaacagcatc acgggaatca cgttgcagtc cgcgttcagg aggcccgtgc
      781 tggtctcgat gtaacgcgtc gattgcgtga gctggttggg ctcgcgcggg atgttgcgcc
      841 acttgagact gccctcgttc cagccggcga tggcgatctt ggcgacgatg ggcagcgcca
      901 tcacctcctg cacgccatcc acgttgcgca tcttccacgc gaaggtgtcg atggccgtca
      961 tgagctcgtg gctggtgcag gccggctcct tcgtctccac gatcaccgtg aggatgtcga
     1021 cgccgatcga gaacttggag gtgatgatgt tgctgtcgcg gttgtaacgc gagtcggcgc
     1081 gcagttccgg cacgcccgac tgagtatcgc cgatgggggt ttcgcgaccc ttccagactc
     1141 cgaagatgcc taacaacacg gcgatgccga tgatgacggc cgcgggcttg cgttcggtga
     1201 tgcgcgccag ccgggcccag tgcacggtta gcttggcctg ccgcagttcg acgcgttgct
     1261 tgtacttgtc gtcccacctt acccacgaca ccatcaccgg cagcaggatg agatcggtga
     1321 gaatgacgat ggcgacgccg atgctggcgg tgaccgccat ttcctgcact acctgtacgg
     1381 gaatcagcag aatcgtgatg aagccgacga ggtcggcgag aagcgcgatg atggccggca
     1441 tcagcaattg ccggaacgtc tggcgcgccg cgtccatgct gctcaggccc gcgaccgccg
     1501 cttccttgac ggcacttatc ttctgcacgc catgcgacac gccgatggcg aagatcagga
     1561 acggcacgag cagccccagg gggtctatgc catagcccag cagcaccagc gtgccgagct
     1621 gccacgtcac cgccacgatg gagcagacga tcggcacaaa cgccactttc agcgactgca
     1681 cgtagtacca gacggccagc agcgtgagca ggatggtgac gatggcgaac acgatgaccg
     1741 acatcgcgcc gtcggcgatg tcacctacca ccttggcgaa gccgatcatg tgcacgtcga
     1801 tgccgccgcc ctgggcctgc accaacacgt cgttgttctg gatcttgtcg cgcacgcggc
     1861 tttcgagatc gcgggccacc tcgatggggt cgatgggttt gccggtggcg tccttctcca
     1921 ggacgatcgc cgacaccagc gcgccggaga agtcgttggc cacgaggcgg ccgcggatgc
     1981 cggccttcag gatgttctcg cgcaccttcg ccagcgcttc gggcgtgggt acgaactccg
     2041 agtcgactac gttgccggcc tcgatgccct cctcgacgac ttccatgtaa cgcacgttgg
     2101 gcgtgaacag cgactgcacg cggctgcggt cgatgccgtc catgaccagc acttcgtcgg
     2161 tggccttctt gagcgcgtcg aagaacccgg gcgtgaacat gttgccgtcg cgcgcaacca
     2221 gcgcgatcag cacgcggttg gcgccgccga actccgcctg gtgcttcacg aaggtctgca
     2281 tgtactcgtg cttgagcggc agctgcttgg tgaacgaggc gtctatgtgc agcccgcgta
     2341 ccgcgaccgc ggccaggccc acggtcagca gcacgaacgc cgcgacgatg gcgacgcgat
     2401 tgccgaagat cagcttctcg agcgtggcgg aaaccttcga atcgcctgcc atgctaacta
     2461 ccttcaccca cgcggatggt gcgcacgccg ccctcgcccg cgacgatgac cgtgcccgcg
     2521 ggccccggca gcagcgccga catgcccttg cgatcgtcct gctgcgtgag cttgaaagtc
     2581 tctccggcgt ccgccgacac cagcagcacg ccggcgagcc cgccgatgac cacgcgcagg
     2641 tcgttgatgg cgatgccgtc ggtcagcatg gcagtggtgt gggactcgag ctcatgccag
     2701 gtctcacccg catcggccga gcggaacagg tggccgcgaa ggccgaatgc cagcaggcct
     2761 tcgccgcgga tcggcaccag cccgaaaaag cttccttggt atggcgaggg caaagctcgc
     2821 cagctcgcgc cgcggtcgtc cgagcgatag agcttcccgc cctcggccgc cacgtacagc
     2881 cggttgccga cccccacgat gcgattgaga tggtagtcag gcggcggttc atcttcattc
     2941 gcgggctgcg tcggcggagc cgagaacctg gcgctggacc agtggcggcc gccgtcgttg
     3001 gtggtgaagt aggcgccgta cgcgccaacg gcaatgccac tgacgcggtt ggcgaaccac
     3061 aggtccagca gaggctgctg tgcttcgggc gcgaaatgcg agcgggtcca gttttcaccg
     3121 ccatcggcgg tgttgaggat ggtctcgtcg tgcccgacgg cccagccgta ctctgcatcg
     3181 acgaagaaga ccgccgtgag aagcacgcgc gtgggcacgc tcttcgcctg gcgccaggtg
     3241 gcgccgtggt catcggacag catcacgtgg ccgcgctcac ccacggcgac gagccgttgg
     3301 ccggcgaccg ccagatcgag cagcagcgaa ctcgacgcca gcggttcgat ttccgccggc
     3361 ctgttggctc catcgttggg atccagctgc gcttgcgtga tgcctggggc aatgccggcc
     3421 acagccagcg tcgcgaccag cgcgaggctc atccgatgcc tcatccgcaa ctcccgtagt
     3481 tcttctgtat ctctgaaact aaacgggccg ggtgatgttg atcaaccggc ccgttaatcg
     3541 cgtttgctaa ctaaaccttc agcggacgcc gcgacgctgc agggctgccg gctggtagtc
     3601 ggcaatggtg cgcttctgtg agaagtcgcg gctgcgcggt tcttcgttgt cgaggccgag
     3661 agcgagataa cggccgttgg agaggtcata caccgtctcg agcgcagtcc acagcgccgg
     3721 cacgtcgtag tactgcatga cgtggccttc ctgcacgcga tagagctggc cgcggctgtc
     3781 gtagcagtcg accgcgacga tgtgccagct gtcttcgtcc acgtacagcg tacggcggct
     3841 gtagaggtgg ctctgtccgg ccttcagctt cgaatccacc acccacacgc gatgcagttc
     3901 ataacgcgcc acgtcggggt tgatgtggtt cttcttgagg atatcggtgt acttgagcgt
     3961 gtttaaattc agcttgtacg cgttgtacgg cacgagcatt tctttcttgc cgaccagcgt
     4021 ccagtcgtag cgctgcgggc tgccgttgta caagtcgaac tggtcggagg tgcgcagcgc
     4081 atccgagttc gtgcccgggt tgtcgaaggc gacgttcggc gcgcgacgga cgcggcgctg
     4141 accggcgttg taaacccagg cgcggcgatt ctcgagcgac tgatcgagcg tctcctgcac
     4201 cagcagcact tcaccggcga ggcgcgccgg cgcgatggtt tcctggatga agtagacgat
     4261 ggtgttgtcg aggttctccg acttcgcacc gggcaggctg taagccgcca tggtgtcgtc
     4321 cctgaacttg accatggtga aggcgccgct cgccgtcacc ggcgcctgac cgatctggcg
     4381 gacgatgacg tcaccgcgat aacgcagcac gtggttccag aacacttcca cgccttcctt
     4441 cgggatggcg aaggggatgc cctcgcccgc gttcgtgaca ccgttgccgc ccttggcgag
     4501 ctgcgcggtg gtggcgatgc gcttggtggc gtcgtagatg cgttgcggag cggcagcgct
     4561 tcgatgcgtg ggatacacgt tcatcttgaa cgtggagccg tacgtctgaa acatcttctt
     4621 gtgaccttcg gtcagcttgc ccgcgtactg agccatgttc gccgacgtca ccgtgtacac
     4681 cggtttgtcg ttggcgtacg gatcaggatg gtgctggccg gggcggaagt tggcgaaacc
     4741 cgcctgcgcg gcggacgtaa gcccgccgtc ccacgcgggg atggtgccgt cggcgttacc
     4801 cgccttcacg ccgccaagcg gcgtgagctc ggcaccgagc ttcgcggcgt cggccgccga
     4861 cacagcagcc acggccgaag acgcaccaat cgcgatgcca gctatcgtcg ccaccatggc
     4921 aagaacatgt tttttcagca tggagat
//
LOCUS       DASQJS010000323        21487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2704971, whole genome shotgun sequence.
ACCESSION   DASQJS010000323 DASQJS010000000
VERSION     DASQJS010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21487
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2704971"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..2482
                     /locus_tag="VEW08_12940"
     CDS             299..2482
                     /locus_tag="VEW08_12940"
                     /inference="COORDINATES: protein motif:HMM:NF015831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HYJ41690.1"
                     /translation="MKIRQLSFASVSLAAFALFSSGAQAQAFDCVDVGGGSIQCGPYS
                     YAQYGYSTAVGESAAAYGYYSTSVGQNAQANGSQSTAIGQNAQAASQSTAIGQQSAAY
                     GYESLALGVNSLAQYNYSIAIGSSAQTYGDQATAVGSHSYSSNSLALAAGWSAYATGF
                     ASTAIGSNSEASNSFGTALGFLSYVDGVNSTALGTNSTVYGGNSTAVGQGSSTYTDNS
                     TALGSNASVTAFGAVAIGNGSLADQANTVSVGTLGNERRIVNVAAPTSATDAANGAYV
                     DAAQSNAQDFATTAAGTAQTNAQTFATAAAGTAQTNAQTFATAAAGTAQTNAQTFATT
                     AAGTAQSNAQAFATTAANAAQTNAQSFATAAAGTAQTNAQSFATIAANTAGSAAQAGA
                     QTYANNLVTGGGTAASVASVNVNNGGIANAGTIGGVTAATASDQAVNFAQLQAVFSQL
                     VQSGLCRIEGATVTCGTTGADNALTAGTNSSVGAGAENAIALGTNSSAQSAGSIAMGH
                     GATAVQSNSVAIGAGAYAQSSVAVGTGAQATGTNTTALGDNANAAGNFAVAVGNNAAA
                     AHDNSVALGNGSTTSAANTVSVGSAGSERRVTNVAAGSGGTDAINLNQLNAALNAVSG
                     GLSAGQAYVDQRFAAANSYTDTQVGYARAFAARGIAASSAIPNIAPSTVGKTAMGLGT
                     GHYDGETAVGLSVAHSFAENFNLSGGVARVSGGKNVARMAVGIEF"
     gene            complement(2499..4346)
                     /locus_tag="VEW08_12945"
     CDS             complement(2499..4346)
                     /locus_tag="VEW08_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HYJ41691.1"
                     /translation="MSGTNPEPGGYRVGDVFIDVGTRQVSRDGSLLQLPPLSFDLLLA
                     LVRRAPNAVSSGELMEKVWTGRVVNDETIAKRVELVREALGDDSRQSRYIALVRGHGY
                     RITADVEPITRPTPATVAAPAMRKRAWPIAIAVVLAAVVIAVMVLRDGAPASRSVATD
                     SVVPQRIALAVLPLDDLSTGNTEEYFAAGMHDALITDLSRASAIKVISRTSTLPYRDT
                     GKSLSTIAGELGVDLVMEGSVLRADDRVRITVQLLDSDDNHLWAEQYEGDVRDVLNLQ
                     SRVARAVAEAVKVKLSPLEHSRMASTRPINVESYDLYLKGMQLLEKSPAEFEAGIGLL
                     QKATEIDPANALAYARLASAYARRAHAPGAAKSLYPRATAYALQAVGLDENLAEAHQA
                     LAEMRLYFHWDWPAAEQSFRKVFELNPNAAATHAHYGWFRMLHDDWPGAIAEAKLAAE
                     LDPNDPLWGCWHAWLYLLHDEYAPAETELRRVLDAHADYPCANHVLGQTLIRLGRHEE
                     ALAAFQKAADASPRWSWGLPQGLALVGRKDEARVLAERLERAEFPDAWGLAEVYTALG
                     DHERALKWVEYGYEIRRDWIPWVKANTFLAPLRTEPRFKEVLRRLDLPT"
     gene            4514..4936
                     /locus_tag="VEW08_12950"
     CDS             4514..4936
                     /locus_tag="VEW08_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41692.1"
                     /translation="MKTLTIALATAMLLSTTAWAGDAPEECSNRMLVGRWMFATDVGE
                     QTFFPGGDITAIGIFRVDRAGNLKGKFDATIANFGFLPGVEFNGTVTVNPDCTGTLTF
                     TTSRGTTRTDSIVVLGYGHVRGMSQDVAFLWTYDMRRL"
     gene            complement(4975..6096)
                     /locus_tag="VEW08_12955"
     CDS             complement(4975..6096)
                     /locus_tag="VEW08_12955"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYJ41693.1"
                     /translation="MSDEQQTARRWRLVLGGDAADGTGYSLDAAELQMDKALNALYDS
                     DRRGGLGSSAPSVARWLGDIREYFPASVVQVMQRDAIERLDMKRLLMEPEMLESLEPD
                     VHLVADLIALSGSIPAKTRETARAVVKKVVDALVAKLAEPTRQAVIGALNRAARNRRP
                     RHADIDWPRTIRANLKHFQPEYQSIIPETRIGFGRRRQSMRHVILCIDQSGSMAASVV
                     YSAIFGAVLATLPAVSTSLVVFDTAIVDLTDELDDPIDLLFSTRLGGGTDINRAVAYC
                     QQLVRAPHETIFVLISDLFEGGVETEFMKRAATLAASGVQFVTLLALSDEGAPHYNAD
                     LAAQLATIGIPAFACTPDRFPELMAAAIQRADLRDFAAK"
     gene            complement(6093..8300)
                     /locus_tag="VEW08_12960"
     CDS             complement(6093..8300)
                     /locus_tag="VEW08_12960"
                     /inference="COORDINATES: protein motif:HMM:NF040143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HYJ41694.1"
                     /translation="MTELHFFGIRHHGPGSARSLVRALQTVRPDVILIEGPPDANELL
                     PLAAHAGLEPPVALLIYLPDRPRAAVLYPFAGFSPEWQAIRYGLAQSVPVRFIDLPQS
                     LRFGEDEDAEDVPAAAARGDPLAPMALAAGHGDTERWWDQLVESREGHDIEVFKAVHE
                     MMGALRTALDEPIPLEEQRREAHMRKCIRAATAEGFERIAVVCGAWHTPALAEMPSAK
                     SDDALLKGLAKTATAAAWVPWSYERLSYRSGYGAGIESPVWYELLWEQRKALGAHWLT
                     RAARLLRDEDVPISSAHVIEACRLAEALASVRGRSVPSLPEYNDAAISVLGNGQALNL
                     RIIERRWHFGDRLGKVPEEFPAAPLAKDLAALQKRLRLPPKAEEKTLDLDLREAFDRE
                     RSHLLRRLRLLGVGWGEPAAHAGGRGTFHELWKLAWKPEFAISLIEASRHGHTVAQAA
                     ANLVAERCAGEDVALKALIALLEDALFADLAAAIGALVAAIENRAALAADVHQLLEAL
                     PPLVAVQRYGNVRETDVSLVAQILHGLVPRVFVAMPPAAVGIDDDAAQFLHQHMVQAD
                     AALANLANEEYLTGWRESLLKISRNDAAHRRIAGYAVRLLYDAHVIEFDLLEKSFALS
                     LSPGSPPAEAAGWTEGFLSGSGALLIHDDKLLGLLNSWLRGIGETHFMQVLPLLRRTF
                     SQFPPAERRQIGERLKPATAAARVVAVDGEFDLEAARAVLPVLKCIWNLGEKT"
     gene            complement(8304..9404)
                     /locus_tag="VEW08_12965"
     CDS             complement(8304..9404)
                     /locus_tag="VEW08_12965"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYJ41695.1"
                     /translation="MKAQTAPDTAVQRAPAEQQFAAELDALAKSDKRQRPTNWKLSPW
                     AVLTYLMGGKADDGTAITPKYVGQRRLMEIAVATLATDRALLLLGLPGTAKSWVSEHI
                     AAAISGDSTLLVQGTAGTAEESIRYSWNYALLLSKGPSRAALVPSPMMRAMESGALGR
                     LEELTRVPADVQDTLISMLSEKTLPIPELAQEVRAARGFNLIATANNKDRGVNEMSSA
                     LKRRFNTVILPTPDSLDEEVSIVQMRVDSIGRSLELPAEAPALAEIRRIVTVFRELRE
                     GKTEDGKSKLKSPSGTLSPAEAISVVTNGMALAGHFGDGSLKAHDVASGLIGAIIKDP
                     AQDGVIWREYLETVVKERDGWKDLYRACRDQL"
     gene            complement(9401..10957)
                     /locus_tag="VEW08_12970"
     CDS             complement(9401..10957)
                     /locus_tag="VEW08_12970"
                     /inference="COORDINATES: protein motif:HMM:NF039502.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HYJ41696.1"
                     /translation="MGADISPFARLAAIATLGTRRAPLPKESPWPDDSLAPAGAEPAT
                     RETCLLRAAAAGAIWERAGTRAGQGAAPLPRDFPAQAGRELDEFAGWRLTRILGGEYP
                     YLLTEWFEAAAATGRPLPPHWLPMVLDHVAPEVRAGFPTVLGPVAAWLAGRNSRWLVA
                     PAGEELSDARWQEGSLEERRLLLAAIRKRDRARSLEWLRSTWESDAPEAREAFLGLLT
                     ADLAADDEPFLEPVLDDKRKPVRLAAADALARLPESAHARRMLARLEPLIEFEAASGG
                     MLAKLRKRKLRIELPTAPDKAALRDGIEAKPPASRKIGERAFWLSQLVAMVPPSHWCR
                     RFDCDAQTFLDAVLATDYASELLGALTQATVRHPQGEWSQALTRAWMASTQETTEVLA
                     ALHDLAMTGSPAERAALLEQILRDTKPANASYISHVLDAAELQWNATITQLAMDHLAG
                     IVASAKENWSHSRNSLDAWARRCDIDIGAHRAAALLDKYGDGHCWRNALEQFNDIVAF
                     RAAMHKELTK"
     gene            complement(10957..12312)
                     /locus_tag="VEW08_12975"
     CDS             complement(10957..12312)
                     /locus_tag="VEW08_12975"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HYJ41697.1"
                     /translation="MGEWSAQRVEQMAPDGAAVKAAQSVARPAKWKSLGCTDRLAWGE
                     CQGSGKDPYQVRVDLVDVTYKCSCPSRKLPCKHTLGLLLLFADGKSVPAGSPPAFVEE
                     WSANRARRAEAKQKKEAEPAATAPDPEAKAKRVEKRENRIGAGLEQLEAWLADIVSQG
                     LAAARAQPPAFWAQMSARLVDAQAPGLANRVELLGDHALASARWQPQLLRSLGELQLL
                     IDAYRNLEGLPPALASEVRTMIGWPQSLDELRERAGVRDIWQVVGRRQTQDQKLRVQQ
                     TWLRGTRGGQLAKVLEFAAGPVPLPVSYTLGQMVDAEMVYFDGCPPLRTLEKLRHGLE
                     PRRLAMAGATDIAGMQTTYATALAENPWMGRAPNMLGPVNPVIEADRLFLCDSAQRRI
                     AVAEGFGHAWALLALAGSDSLQLFGEWDGLEFDPLTIECRGAWYTPAQLATLPILSRV
                     A"
     gene            complement(12329..15031)
                     /locus_tag="VEW08_12980"
     CDS             complement(12329..15031)
                     /locus_tag="VEW08_12980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HYJ41698.1"
                     /translation="MKLLTARQPVLLACLSACATISVAADPPAATPAPTVVPLDIFSV
                     PEGLEVTLWARSPMLKNPTNIDIDAAGRIWVTEGVNYRRHKERDPKGDRVVVLEDTDR
                     DGRADKSTTFVQEPTLVAPLGIAVIGNKVIVSNAPDLIVYTDVDGDRLFNPAIDKREV
                     LLSGFDGRNHDHALHSVTFGPDGRWYFSQGNAGANFTDRGGKTFRVGSWYNPVPDGGA
                     QAVFGSAPLSYAGAKSDDGHVYVGGFSARMQVDGTGVEILGFNFRNSYEQTVTSFGDI
                     FHNDNDDPPACRTTYLMEYGNLGFFSRDGKRSWQADRRPGQSIPTAEWRQEDPGVIPS
                     GDVYGGGAPTGIVFNEGDGLGRKWRGLLLSAEAARNTIFGYFPKPQGAGYLLDRMDFL
                     TSNAAQQFAGTDFKGGKVNSEIQTFFRPSDVAVGPDGAIYVADWFDPRVGGHDDKDDT
                     TSGAIYRIAPKGFKSVIPAFDASTLEGQMTALRSPAVNVRATGFSGLIERGTAAIPAL
                     EVLLRDDNPFIRARAVWVMARLGVSGLARVESLTSDADPMMRIVAYRTLRQMRQSMRF
                     ADQLARDPSPAVRREVALSMRDVPIYSSRDILLTLAARYDGKDRSYLEAWGIGATNKE
                     EPLYTALAATQAEPDATKWTQGYSDLVWRLTPRAAVAVFAARAGATTLPAAERSKAIT
                     ALGFIPTAEAANALLDIAQHGGSDIKQNPALWWLINYKDSRWAGQGIDAELKRRGLYD
                     PDSVAISEVMVPAPTAKSALPAVTDIAKLRGDPKRGAVAAGACLMCHRIQGKGVDYAP
                     ALDGFASRQTRDVVITAIVNPSNDIAHGYEGTELTLNDGRTIHGLILSGGNPVIVQST
                     GGVTQMVPAGLIKERKRLGRSLMLSADQLGLNAQQVADIAAYLR"
     gene            complement(15072..15980)
                     /locus_tag="VEW08_12985"
     CDS             complement(15072..15980)
                     /locus_tag="VEW08_12985"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HYJ41699.1"
                     /translation="MDNRRTFLQQSAAAVSLAALAGASTAARKKPGSRQFKLRYAPHF
                     GMFKEHAGPDPIAQLEFMAAQGFTALEDNGLMGRPVELQQRIGETLARLGMTMGVFVI
                     DGGDNWKTSLTTGEAQFREKFLDTCRAAVEVGKRVNARWATVVPGYFERKLPIGIQTG
                     HVVDTLRAGAEILAPHNLTMVLEPLSDNPDLFLRTSDQAYAICRAVDSPACKILYDMY
                     HMQRNEGDIVQHINLAWSEIAYFQIGDNPGRKEPGTGEMNYRNLFRHISGKGFNGIFG
                     MEHGNAKPGKEGEQALIDAYVAADSF"
     gene            16088..17071
                     /locus_tag="VEW08_12990"
     CDS             16088..17071
                     /locus_tag="VEW08_12990"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ41700.1"
                     /translation="MISTKRLLFNAATFIPGVAQLPPVKRVFKRHSIGTHGTIEARYC
                     YSVWLRHLMCAAANDLDTNPRAVAELGPGDSIGIGIAAVLTGVERYFAFDVVAHANTP
                     RNLAIFDGLVDLFLEQIDIPDASEYPRVGPTLKDYRFPGHIFTPDRMREALDPARLAY
                     VRESIQRTDQKDSMIQYRAPWSSSGAVEHETLDLVYSQATLEHVDGLPEVYRAMYQWL
                     KPGGYMSHQIDFKCHNSAREWNGHWTYSKLMWKLVRGKDVWLINRLPYSTHVELMEKA
                     GFRIVGEQLVRRASNVRRRQLASQFRSIPDSDLTTTDAFVQAVKTVPGTDC"
     gene            complement(17092..18102)
                     /gene="rtcA"
                     /locus_tag="VEW08_12995"
     CDS             complement(17092..18102)
                     /gene="rtcA"
                     /locus_tag="VEW08_12995"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF003246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HYJ41701.1"
                     /translation="MIEIDGSEGEGGGQILRSSLSLAICTQQPFRITNIRANREKPGL
                     MRQHLTAVKAAAEICAAEIEGAELGARALTFRPGRLAAGDYSFAIGTAGSCTLVFQTV
                     LPALLTAGGVSRVRITGGTHNRASPPFDFLARSFLPLLGRMGADVQLDLTSYGFYPRG
                     GGEIRARITPGARLKALNLLERGAWVRGYAEAYVSAIPIHVAQRELEVIGRALGWSRE
                     QLLLRGLPNDVGPGNALTITLEYENVIEVFTGFGEKGVRAEIVAGQAAEAARHYLSAV
                     APVGEHLADQLLLPMALGDGGSFVTAQATPHLRSNVAVIERFTARRIAIAPVKSGFEI
                     SL"
     gene            complement(18116..18754)
                     /locus_tag="VEW08_13000"
     CDS             complement(18116..18754)
                     /locus_tag="VEW08_13000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ41702.1"
                     /translation="MKTAAVFVHGLWLTGAESALMRRRIAAKHGIACHSFTYRTVGSS
                     VQPVLAALAQFVNRIDADRVHFVGHSLGGIVLHRYFEQAKDLPPGRVVFLGSPTVRSK
                     TAERVGRLPVFRSMIGRMVTEELVQPADAREWRCDRELGCIAGTRPMGLGRFFARFDE
                     DSDGTVGVGETRLPGHSAHLTLPVSHMGMLLSADVARQVGEFLENGRFIARS"
     gene            complement(18780..20555)
                     /gene="aspS"
                     /locus_tag="VEW08_13005"
     CDS             complement(18780..20555)
                     /gene="aspS"
                     /locus_tag="VEW08_13005"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HYJ41703.1"
                     /translation="MRSHYCGQVNEKLVGQEVTVAGWVHRRRDHGGVIFVDVRDREGL
                     LQVVFDPDNQAQFNEAEKLRNEFVVQVRGLVRERPAGTANANLTSGKVELLARELVTL
                     NRAEPLPFQLDEHVSEEVRLKYRYIDLRREDMSKRLRLRHKITRAMRHYLDDAGFVDI
                     ETPVLTKATPEGARDYLVPSRTHAGKFFALPQSPQIFKQLLMVAGFDRYYQIVKCFRD
                     EDLRADRQPEFTQLDIETSFLTQDQIMELMEKLTRHFFKDGIGVDLAEPFPRMAYAEA
                     MRRFGSDKPDLRNPLELVDVADLVAGSDFKVFAGPAKDPKGRVAALRVPGGGAMPRSQ
                     IDEYTAFVARYGAKGLAYIKVNETAKGKEGLQSPIVKFLSDESVKGILDRTGAKDGDL
                     IFFGADSAKVVSDAIGALRLKVGVDQKLTSEGWKPLWVVEFPMFDYDPDEKRWVAMHH
                     PFTAPVDDDVAALKAFPSGALAKAYDLVLNGSEIAGGSVRIFRQDMQSAVFDLLGIGA
                     EEAQLKFGFLLDALKFGAPPHGGIAFGLDRLVMHMAGTDSIRDVIAFPKTQTAADPLT
                     DAPTEISEKQLREVHIRVRTPPPTS"
     gene            20743..21444
                     /locus_tag="VEW08_13010"
     CDS             20743..21444
                     /locus_tag="VEW08_13010"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HYJ41704.1"
                     /translation="MTRKLVAMLAITVAGLAAVASAQAQTWVAGKHYTVIPTQRTNVP
                     AGKVEVMEVFSYGCPACNSFLPTMKKLKAALPPNAQLVYLPASWNKSENWPLFQRAYL
                     TAQSLGVAEKAHEVMFQAIWSTGELGVTEPSGRLKKVLPSIEDTARFYERTTGVKAAT
                     FVNASKSFGVDLKMRQADSQIIAMQVSGTPTLVVNGKYRLNNDSLTTEQMVDLVKFLV
                     AKESAPAAAAPAKKG"
BASE COUNT         3707 a         7144 c         6953 g         3683 t
ORIGIN      
        1 ccgcggctgc tcgagctcga agcggcactg cgcgcggtca gcaccgacga tctcagtccg
       61 cgcgcggcgc tcgacctggt ctacaagctg cgagcacttc tgggtccggc aacctaagcc
      121 ctaagggcac agagccaagg gttcgatcgc gaacttcgtc atctaactag tgtgacgcac
      181 tacgcggcgc gaagattcgc cgcacttatg gcatgaagac ccttgctacc ctgctccacg
      241 cccgggatcc aaccgcgcgc acgcactgcc gcggtcactc gaacccaagg atccttccat
      301 gaaaatccgt caattgtcgt ttgccagtgt gtcgctggca gcgtttgccc tgttcagttc
      361 cggcgcgcag gcacaggcgt tcgactgcgt cgacgtcggg ggcggatcta tccaatgcgg
      421 accctactct tatgcgcaat acggctacag cacggccgtg ggtgaaagcg cggccgcgta
      481 cggctactac agcacttcgg tcggccagaa cgcccaggca aacggctctc aaagcacggc
      541 gatcggccag aacgcccaag ccgcctcgca aagcacggcg atcggccagc agtctgccgc
      601 gtacggttac gaatcgttgg ccctgggcgt gaactcactg gcgcaataca actacagcat
      661 cgcaatcggc agctccgcac agacatacgg cgaccaggcc actgcggtcg gcagccattc
      721 gtattcatcg aactcgttgg cacttgcggc cggctggtcc gcgtatgcga caggcttcgc
      781 gagcaccgcg atcggcagca actctgaggc cagcaactcg ttcggcacag cgcttggctt
      841 cctcagctac gtggacggag tgaactccac cgcgttgggt acgaacagca ccgtgtacgg
      901 cggtaatagc accgcagtcg gacaaggtag ttcgacatac acagacaaca gcaccgcgct
      961 cggttccaat gcctcggtca cggccttcgg cgcagtggcg atcggcaacg gctcgttggc
     1021 ggatcaggcc aacaccgtga gcgtgggcac gttgggcaac gagcggcgca tcgtcaacgt
     1081 tgctgcgccc acgagcgcga ccgacgccgc gaatggcgcg tacgtggacg ctgctcaaag
     1141 caacgcgcaa gacttcgcga ctaccgccgc gggcactgct caaacgaacg cgcagacctt
     1201 cgctaccgct gccgccggca ctgctcaaac gaacgcgcag accttcgcta ccgccgccgc
     1261 aggcaccgct caaacaaatg cgcagacctt cgcgaccaca gccgcgggca ccgcgcagag
     1321 caacgcgcag gctttcgcta ccacggcggc gaatgccgcc caaacgaacg cgcagagttt
     1381 cgcgacggcg gccgcgggca ccgcgcaaac gaacgcgcag tctttcgcga ccatcgccgc
     1441 caacacggcg ggatcggcgg cgcaggccgg cgcgcagacc tacgctaaca acctcgttac
     1501 cggcggcggc actgccgcga gcgtcgcgag tgtcaacgtg aacaacggcg gcatcgcgaa
     1561 tgccggcacg atcggtggag tcaccgcggc aaccgccagc gaccaggcag tgaacttcgc
     1621 gcagctgcaa gcggtattca gccagctggt gcagagcggc ctgtgccgca tcgaaggcgc
     1681 gacggtgacc tgtggcacga caggggctga caatgccctg accgccggta cgaactcgag
     1741 cgtcggcgca ggtgccgaaa atgccatcgc actcggcacg aactccagcg cgcaaagtgc
     1801 gggctctatc gcgatgggtc acggcgcaac cgccgtgcag tcgaactcgg tggccatcgg
     1861 cgcgggcgcc tatgcgcaat cgtcggtggc ggtgggcacg ggcgcccagg ccacgggcac
     1921 caacacgacc gcgctcggcg acaatgccaa cgcggcggga aatttcgccg tggccgtggg
     1981 caacaatgcc gccgccgcgc atgacaattc cgtcgcgctc ggcaatggct cgaccaccag
     2041 cgcggcgaac acggtgtccg tcggttcggc cggcagcgag cgccgggtca ccaacgtggc
     2101 cgccggctcc ggcggcacgg atgcgatcaa cctcaaccag ctcaacgcgg cactcaatgc
     2161 cgtgagcggt ggattgagcg cggggcaggc ctacgtggat caacgcttcg ccgccgcgaa
     2221 ctcgtatacc gacacgcagg tgggttatgc gcgcgcgttc gccgcccgcg gcatcgccgc
     2281 gagctcggcg attcccaata tcgctccgtc cacggtgggc aagacagcca tgggtctggg
     2341 cacgggccac tacgacggcg agacggctgt cggcctgtcg gtggcgcact cctttgccga
     2401 gaacttcaat ctttccggtg gcgtcgccag ggtcagcggc ggcaaaaacg tcgcgcgcat
     2461 ggccgtcgga atcgagttct agcgcaggcg ctgctcggct aggtgggcag atcgagcctg
     2521 cgcaagactt ctttgaaccg gggttccgtg cgcagcggcg cgaggaaggt gtttgccttc
     2581 acccagggaa tccagtcgcg gcggatttcg tagccgtact cgacccactt caacgcgcgc
     2641 tcgtggtcgc cgagcgcggt gtacacctcc gcgagtcccc aggcatcggg aaactcagcg
     2701 cgttcgaggc gttcggcgag cacgcgggct tcatccttgc gaccgaccag ggccaggcct
     2761 tgcggaagtc cccacgacca gcgtgggctc gcgtcagcgg ccttttgaaa ggccgccagc
     2821 gcctcttcgt gccggccgag ccggatcaaa gtctgcccaa gcacatggtt cgcgcagggg
     2881 tagtcagcat gcgcgtcgag cacccggcgc agttcggttt cggccggcgc gtactcgtca
     2941 tgaagcaggt acagccaggc gtgccaacac ccccaaagcg gatcgtttgg gtcgagctct
     3001 gcggcaagtt tcgcctccgc aatcgcgccc ggccagtcgt cgtgcaacat gcgaaaccag
     3061 ccgtagtgcg cgtgtgtcgc ggcagcattg ggattgagct cgaaaacttt gcgaaatgat
     3121 tgttcggccg cgggccaatc ccaatggaag tagagccgca tctcggccag cgcctggtgc
     3181 gcttcggcga ggttttcatc caggcccacg gcctgcagcg cgtacgccgt ggcgcgcggg
     3241 tacaacgact tcgctgcgcc cggcgcgtgg gcgcgccggg catacgccga cgcaaggcgc
     3301 gcataggcca gggcgttggc gggatcgatt tcagtggcct tctgcagcaa cccgatgccc
     3361 gcctcgaatt cggcgggtga tttttcgagc aactgcattc ctttcagata caagtcgtaa
     3421 ctttcgacgt tgattggccg tgtcgacgcc atgcgcgagt gttccagagg cgacagtttg
     3481 actttcacgg cctccgccac cgcacgcgcc acgcggctct gcaggttcag cacatcacga
     3541 acgtcaccct cgtattgctc cgcccacagg tggttgtcat cggagtcgag caactgcacg
     3601 gtgatgcgaa cgcggtcatc ggcacgcagc accgagcctt ccatgaccag atccacaccc
     3661 agctccccgg cgatcgtcga cagactcttg ccggtgtcgc ggtatggcag cgtcgaggtg
     3721 cgcgaaatca ccttgatggc gctggctctc gacagatcgg tgatcaacgc gtcgtgcatg
     3781 cctgcggcga agtattcctc ggtgttgcca gtggacaggt catccagcgg cagcacggcg
     3841 agcgcgatgc gctgcggcac aacggaatct gtcgccactg aacgagatgc cggcgcgcca
     3901 tcgcgcagga ccatcaccgc aatcaccacg gctgcgagaa cgaccgcaat cgcgatcggc
     3961 catgcgcgct tgcgcattgc gggcgcggca acggtggcgg gtgtggggcg cgtgatgggc
     4021 tccacgtccg cggtgatgcg atatccgtgg ccacggacca gcgcgatata ccgcgattgc
     4081 cggctatcgt cgccaagcgc ctcgcgcacc agctcgacgc gcttggcgat ggtttcgtcg
     4141 ttgacgaccc ggccggtcca gaccttttcc atgagctcgc cgctgctcac cgcgttcggc
     4201 gcgcgccgta ccagcgccag cagcagatcg aaagacaggg gtggcagctg caacaggctg
     4261 ccatcgcggc tgacctgccg cgttcctaca tcgatgaata catccccgac gcggtaaccg
     4321 cccggttccg ggtttgtccc tgacatcttt tctccccgaa agggagtgtt tgtgccgtcg
     4381 atactagtga cttcgcgcgg ccttcgcgaa ttcttcgaga acatttcgcc ccgtcttcag
     4441 gcagttaggc gaccacggtt gccacagttc gccccgacga acccacaacc gcaacccgct
     4501 cgaggagaat ccgatgaaga cgctcaccat tgctctcgca accgccatgc tgctcagcac
     4561 caccgcatgg gccggcgatg ctcccgaaga atgcagcaac cgcatgctcg tcggccgctg
     4621 gatgtttgcc accgatgtgg gcgaacagac cttctttcct ggcggcgaca tcaccgccat
     4681 cggcatcttt cgcgtggacc gcgccggcaa cctgaagggc aagttcgacg ccaccatcgc
     4741 gaacttcggt ttccttcccg gcgtggaatt caatggcacg gtcaccgtga atccggattg
     4801 caccgggacc ctgactttca ccaccagccg cggcaccacc cgcaccgaca gcatcgtcgt
     4861 gctcggatac ggtcacgttc gtggcatgtc gcaggacgtc gcgttcctgt ggacctacga
     4921 catgcgccgc ctgtgaggca gcccggggcg tgcacgcaga caactgcgtg ccgcctactt
     4981 cgccgcgaag tccctgaggt cggcgcgctg gatggcagcg gccatcaact cgggaaagcg
     5041 gtccggcgta cacgcgaacg cggggatgcc aatggtggcg agctgcgcgg cgaggtcggc
     5101 gttgtagtgc ggcgcgccct cgtccgacag cgccagcagc gtgacgaatt gcacgccgga
     5161 cgcggcaagg gtggcggcgc gcttcatgaa ttcggtctcg acgccgcctt cgaacagatc
     5221 gctgatcagc acgaagatcg tctcgtgcgg cgcacgaacc agctgctggc aataggcgac
     5281 agcgcgattg atgtccgtgc cgccgccgag tcgcgtggaa aacagcagat cgatgggatc
     5341 atcgagctcg tcggtgagat ccacgatggc ggtatcgaac acaaccagcg aagtggagac
     5401 ggccggcagc gtcgcgagca ccgcaccaaa gatggccgaa tacaccacgc tcgccgccat
     5461 cgaaccgctc tggtcgatgc acaggatcac gtgacgcatc gactgacggc gccgcccgaa
     5521 accgatgcgc gtttcgggaa tgatgctctg gtattcgggc tggaagtgct tgagattggc
     5581 tcgaatggtg cgtggccagt cgatgtcggc atggcgcggg cggcggttgc gcgccgcgcg
     5641 gttcagcgct ccgatcacgg cctgccgcgt gggttcggcg agtttcgcca ccagagcatc
     5701 gactactttc ttcaccacgg cccgcgcagt ctcgcgcgtc ttcgcgggaa tcgaacccga
     5761 cagcgcgatc agatcggcca ccaggtgcac atctggctcg agcgactcga gcatctccgg
     5821 ctccatgagc aggcgcttca tatcgagccg ctcgatggca tcgcgctgca tgacctgcac
     5881 gacgctggcc gggaaatatt cgcggatgtc gccgagccag cgtgcgacgc tcggcgcgga
     5941 actgccaagc ccgccgcgcc ggtcggagtc gtagagcgcg ttcaacgcct tgtccatctg
     6001 caattccgcg gcgtcgagcg aatagccggt gccgtccgcg gcatctccgc cgagcacgag
     6061 gcgccagcgg cgcgcggttt gctgctcgtc gctcacgttt tctctccgag gttccagatg
     6121 catttcagaa ccggcagcac ggctcgcgcc gcctcgagat cgaattcccc gtcgacggcc
     6181 accacgcgcg cggcggcggt ggcgggcttg agccgctcgc cgatctggcg ccgctcggcg
     6241 ggcggaaact gcgagaacgt ccgccgcagc agcggcagca cctgcatgaa gtgggtctcg
     6301 ccgattcccc gcagccacga gttcagcagc ccgagtagtt tgtcgtcgtg gatcagcagc
     6361 gcgccgctgc cggagagaaa gccttcggtc caaccggccg cttccgcggg cgggctgccg
     6421 ggcgagagcg acagcgcgaa ggatttttcg agcaagtcaa attcgatgac atgcgcgtcg
     6481 tacagcagcc gcaccgcata cccggcgatg cggcggtgcg cggcgtcgtt gcgggagatt
     6541 ttcaacagtg actcgcgcca gccggtgagg tattcctcgt tcgcgagatt ggcgagcgca
     6601 gcatcggcct gcaccatgtg ctgatgcagg aactgcgcgg catcgtcgtc gatgcccacg
     6661 gccgcgggcg gcatcgcgac gaacacgcgc ggcaccagtc cgtgcagaat ctgcgccacc
     6721 agcgacacat cggtttcacg cacgttgccg taacgctgca cggccaccag cggaggcagc
     6781 gcttccagca gctggtgcac gtccgctgcc agtgcggcgc gattctcgat ggccgccacg
     6841 agcgcgccga tggcggccgc aaggtccgcg aacagcgcat cttccagcag tgcgatgagc
     6901 gccttgagcg cgacgtcttc gcccgcacaa cgctccgcga ccaggttggc cgcggcctgc
     6961 gccacggtgt ggccatgccg gcttgcttca attaacgata tggcgaactc gggtttccac
     7021 gccagcttcc acaactcgtg gaacgttcct cggccgcccg cgtgcgccgc gggctcgccc
     7081 catcccacgc ctaacaatcg caaccggcgc agcagatgac tgcgctcgcg atcgaaagct
     7141 tcgcgcagat cgaggtcgag cgtcttttct tccgccttcg gcggcagccg caggcgcttc
     7201 tgcagggccg ccaggtcctt cgcgagtggc gcggctggaa attcctcggg aaccttgccg
     7261 aggcggtcac cgaaatgcca gcggcgctcg atgatgcgca ggttcagcgc ctggccgttg
     7321 ccgagcaccg agatcgccgc gtcgttgtat tcgggcaggg acggcacgga acgcccgcgc
     7381 acggacgcga gcgcctcggc caggcggcag gcttcgatga cgtgcgccga ggaaatcggc
     7441 acgtcctcgt cgcgaagcag gcgtgcggcg cgcgtcagcc agtgcgcgcc caatgccttg
     7501 cgctgctccc acagcaactc gtaccagaca ggcgactcga tgcccgcacc gtagccggaa
     7561 cgataggaca atctttcgta cgaccacggt acccaggcgg ctgccgtggc cgtcttcgcc
     7621 aggcccttga gcaaggcgtc atcggatttt gcgctcggca tctcggcgag tgccggtgta
     7681 tgccaggcgc cgcagactac cgcgatgcgt tcgaatcctt ccgcggtggc ggctcgaatg
     7741 cacttgcgca tgtgcgcctc gcgccgctgc tcttcgagcg gtatgggctc gtcgagcgcc
     7801 gtgcgcagcg cgcccatcat ctcgtgcacc gccttgaaca cctcgatgtc gtgaccttca
     7861 cgcgattcca cgagctggtc ccaccagcgc tcggtatcgc cgtgcccggc ggccagcgcc
     7921 attggtgcga gcgggtcgcc gcgcgcggca gccgcgggga cgtcctcagc gtcttcgtct
     7981 tcaccaaagc gcagcgactg cggcaggtcg atgaagcgca ccggtaccga ctgggccagg
     8041 ccatagcgaa tggcctgcca ctcgggcgag aaacccgcga agggatacaa cacggcggca
     8101 cgcggccggt ccggtagata gatgagcagc gccaccgggg gttcgagccc ggcatgcgcg
     8161 gcgagcggga gcagttcgtt ggcgtccggg ggaccttcga tgaggatgac gtcgggccgc
     8221 accgtctgca gcgcgcgcac caggctgcgg gcggagcccg gcccgtggtg gcgaatgccg
     8281 aagaaatgca gctcggtcat tggtcagagc tggtcgcggc aggcgcggta gagatccttc
     8341 cagccgtcgc gttccttgac caccgtctcc agatattcgc gccagatcac gccgtcctgc
     8401 gccggatcct tgatgatcgc gccgatcagg cccgacgcca cgtcatgtgc tttcaggctt
     8461 ccatcaccga agtgacccgc gagcgccatg ccgttggtga ccacgctgat ggcctcggcg
     8521 ggcgacaacg tgccgctcgg cgacttgagt ttcgacttgc cgtcctcggt cttgccttcg
     8581 cgcaactcgc gaaacacggt gacgatgcga cggatctccg cgagcgccgg tgcttcggcc
     8641 ggcaactcga gcgagcgccc gatcgaatcg acgcgcatct gcacgatgga cacttcctcg
     8701 tcgagcgaat cgggcgtcgg caggatcacc gtgttgaagc ggcgcttgag cgcgctcgac
     8761 atctcgttga cgccgcgatc cttgttgttc gccgtggcga tcaggttgaa gccgcgcgcc
     8821 gcgcgcactt cctgcgccag ctccgggatg ggcagcgtct tttccgacaa catcgatatc
     8881 aacgtgtcct gcacgtcggc gggaacacgc gtgagttcct cgagccgccc gagcgcgccg
     8941 ctttccatcg cgcgcatcat cgggctcggc accagcgccg cgcggctggg tcccttggag
     9001 agcagcagcg cgtagttcca gctgtagcgg atggactcct ccgcggtgcc cgccgtgccc
     9061 tgcaccagca gcgtggagtc gccgctgatc gccgccgcga tgtgctcgct gacccagctc
     9121 ttggccgtgc cgggcagccc taacaacagc agcgcgcggt cggtggcgag ggtcgcgacg
     9181 gcaatttcca tgaggcggcg ctgcccgacg tatttcggcg tgatcgcggt accgtcatcc
     9241 gccttgccgc ccatcagata agtgagcacc gcccacgggg acagcttcca gttggtcggc
     9301 ctctggcgct tgtcgctctt cgcgagcgcg tcgagttccg cggcgaattg ctgttcggcg
     9361 ggcgcgcgtt gcacggcggt gtcgggggcg gtttgggctt tcacttggtg agctccttat
     9421 gcatcgcggc gcgaaatgcc acgatatcgt tgaattgttc cagtgcgttg cgccagcaat
     9481 ggccatcgcc atatttgtcg agcagcgcgg cggcgcggtg cgcgccaatg tcgatgtcgc
     9541 agcgtctcgc ccaggcatcg aggctgttgc gcgaatgtga ccagttctct ttggcgctgg
     9601 cgacgatacc ggccaggtga tccatcgcga gctgcgtgat cgtcgcgttc cactgcagct
     9661 cggccgcatc gagcacgtgc gagatgtagc tcgcgttggc cggcttcgtg tcccgcagaa
     9721 tctgttcgag cagcgcagcg cgttcggccg gagagccggt catcgccagg tcgtgcagcg
     9781 ccgccaggac ctccgtcgtc tcctgggtcg acgccatcca tgcgcgcgtc agcgcctgcg
     9841 accactcgcc ttgcgggtgg cgcaccgtgg cctgcgtgag cgcgccgagc agctcgctcg
     9901 cgtagtcggt ggcgagcacg gcatcgagaa aggtctgtgc gtcacagtcg aaacgacggc
     9961 accagtgcga cggcggcacc atcgccacca attgcgaaag ccagaacgcg cgctcgccga
    10021 tcttgcggct cgcgggtggt ttggcttcga tgccgtcgcg gagtgccgcc ttgtcgggcg
    10081 cagtcggaag ctcgatgcgc agcttgcgct tgcgcaactt cgccagcatg ccgccactcg
    10141 cggcttcgaa ttcgatcagc ggttcgagcc gcgcgagcat gcgcctggcg tgcgccgact
    10201 cgggcaggcg ggcaagcgcg tccgcggccg cgaggcgcac cggcttgcgc ttgtcgtcga
    10261 gcacgggctc gagaaacggc tcatcgtccg ccgcgagatc ggcggtgagc aggcccagga
    10321 acgcctcgcg tgcttcgggc gcatcgcttt cccacgtgga acgcagccat tcaagcgaac
    10381 gggcgcgatc gcgtttgcga atcgcggcca gcaacaaccg tcgctcctcg agcgaccctt
    10441 cctgccagcg tgcatcggac agctcctcgc cggcgggcgc gacgagccag cgcgaattgc
    10501 ggccggcgag ccaggcggcg acgggcccga gcacggttgg gaatccggcg cgcacttcgg
    10561 gcgcgacatg gtcgagcacc atgggcaacc aatgcggcgg cagagggcgg ccggtggcgg
    10621 cggccgcctc gaaccattcg gtgagtagat agggatactc gccgcccagg attcgagtga
    10681 gccgccaacc ggcgaattcg tcgagctcgc gcccggcctg cgcggggaaa tctctcggca
    10741 aaggcgccgc gccctggccc gcgcgagtgc cggcccgctc ccagatcgcg ccagcggcgg
    10801 cggcccgcaa caggcaggtc tcacgagtgg ccggttccgc accggcgggc gccagcgagt
    10861 catccggcca gggcgattcc ttgggcaagg gtgcgcgccg cgtacccagt gtcgcgatcg
    10921 cggcgagccg cgcaaacggt gaaatatcgg cgcccatcag gccaccctag acaggatggg
    10981 cagggtcgcg agttgcgcgg gtgtgtacca cgcgccacgg cactcgatgg tgagcgggtc
    11041 gaattccaat ccatcccact cgccaaacaa ctgcagcgag tctgaaccgg caagcgccag
    11101 cagggcccac gcgtgtccga atccttcggc aaccgcgatg cgccgctggg ccgaatcgca
    11161 gagaaacagc cggtcagcct cgatcaccgg attcaccggg ccgagcatgt tcggcgccct
    11221 gcccatccag gggttttccg cgagcgcggt cgcgtaagta gtttgcatgc ccgcgatgtc
    11281 ggtggcgccc gccattgcga gccgccgcgg ttccagcccg tggcgcagtt tttccagcgt
    11341 gcgcagtggc ggacagccat cgaaatacac catctcggca tccaccatct ggccgagcgt
    11401 gtaactcacg ggcaggggaa cgggtccggc ggcgaattcg agcaccttcg caagctggcc
    11461 gccgcgagtg ccgcgtaacc aggtctgctg cacgcgaagt ttctgatcct gcgtctggcg
    11521 ccgcccgacc acctgccata tatcgcgcac gccggcgcgc tcgcgcaact cgtccaggga
    11581 ttgcggccag ccgatcatgg tgcgcacttc tgacgcgagc gctggcggca agccctcgag
    11641 gtttcgatag gcgtcgatga gcaactgaag ctcgccgagg ctgcgcagca gctgcggctg
    11701 ccagcgcgca ctcgcaagcg cgtgatcgcc gagtagttcg acgcggttcg cgaggcccgg
    11761 ggcctgcgca tccaccagcc gcgcggacat ctgcgcccag aacgcgggtg gctgtgcccg
    11821 cgccgcagcc agcccctgcg agacgatgtc ggcaagccag gcctcgagct gttcgaggcc
    11881 cgccccgatg cggttctcac gcttctcgac gcgcttggcc ttcgcctcgg ggtccggcgc
    11941 agtagcggct ggctccgcct ctttcttttg cttggcctcg gcgcgcctgg cgcggttcgc
    12001 ggaccattct tcgacgaatg ccggtgggga acccgccgga accgacttgc catcggcgaa
    12061 cagcagcagc aatcccaacg tatgtttgca gggtagtttg cggctcgggc agctgcactt
    12121 gtaagtgacg tcgacgaggt cgacgcgcac ctggtagggg tctttgccac tgccctggca
    12181 ttcgccccag gccagccggt cggtgcaacc gaggcttttc catttcgcgg gccgcgccac
    12241 cgactgcgcc gccttgacgg cggcgccatc gggcgccatc tgttcgacgc gctgcgcgct
    12301 ccactctccc atggccgaag tatagggatc agcgcagata cgcggctatg tccgccacct
    12361 gctgcgcatt gagtcctaac tggtcggcgc tcagcatgag tgaacgtccc aggcgtttgc
    12421 gctccttgat gagcccggcg ggaaccatct gggtcacccc accggtgctc tgcacgatca
    12481 ccgggttgcc gccgctcagg atgagaccgt gaatggtcct gccatcgttg agcgtgagct
    12541 cggttccctc gtaaccgtgc gcgatgtcgt tcgagggatt cacgatggcc gttatgacca
    12601 cgtcacgtgt ctgccggctg gcgaaaccat cgagcgcggg cgcgtagtcc acgccctttc
    12661 cctggatgcg gtgacacatg aggcaggcac cggcggctac ggctccccgt ttcgggtcgc
    12721 cgcgcagttt ggcgatgtcg gtcaccgcgg gcagcgcgga tttcgccgta ggcgcgggga
    12781 ccatgacttc gctgatcgcg accgagtccg ggtcatagag accgcgacgc ttgagttccg
    12841 catcgatgcc ctgccccgcc cagcgcgagt ccttgtagtt gatgagccac caaagtgccg
    12901 ggttctgctt gatgtcgctg ccaccgtgct gggcgatatc gagcaaagcg ttggccgctt
    12961 cggcagtggg aatgaagccg agcgccgtga tcgccttgct gcgttccgct gcgggcagcg
    13021 tggtggcgcc ggcacgagct gcgaacacgg caacggccgc gcgcggcgtg agacgccaga
    13081 cgaggtccga atacccttga gtccatttcg tggcgtcggg ctcggcttgc gtggcagcga
    13141 gcgccgtgta cagcggctcc tctttatttg tcgcgccgat gccccaggcc tcgaggtagc
    13201 tgcgatcctt gccgtcgtaa cgtgcggcca gcgtgagcag gatgtcgcgc gaggaataga
    13261 ttggcacgtc acgcatggac agcgccacct cgcggcgcac cgccggtgag ggatcgcgcg
    13321 ccagctggtc cgcgaagcgc atggattggc gcatctgccg cagcgtgcga taggcaacga
    13381 tgcgcatcat gggatcggcg tcggaggtca gggattcgac gcgagcgagc ccgctcactc
    13441 ccagtcgcgc catgacccac accgcgcgtg cgcggatgaa cgggttatcg tcgcgcagca
    13501 agacttccag cgccgggatc gcggcggtgc ctcgctcgat gaggccggag aaacccgtcg
    13561 cacgaacatt gaccgcgggg ctgcgcagtg cagtcatctg cccttcgagc gtgctcgcgt
    13621 cgaacgccgg aatgacggat ttgaatccct tcggcgcgat gcggtagata gccccggacg
    13681 tggtgtcgtc cttgtcgtcg tgtccgccga cacgcggatc gaaccagtcg gcgacgtaga
    13741 tggcgccatc cggacccacg gcgacgtcgg agggacggaa gaaggtctgg atctccgaat
    13801 tgaccttgcc acccttgaag tccgtgcccg cgaactgctg cgcggcattg ctggtcagga
    13861 aatccatgcg atcgagcagg tagcccgcgc cctgtggctt cgggaaatag ccgaagatgg
    13921 tgttgcgcgc cgcttccgcc gacagcagca gcccgcgcca tttcctgccc aggccatcgc
    13981 cttcgttgaa tacgatgccc gtgggcgcgc ctccgccata aacatcgccc gagggaatca
    14041 cgcccggatc ttcctggcgc cattcggccg tgggaatgga ttgaccgggg cggcggtccg
    14101 cctgccacga acgtttcccg tcacgcgaga agaaaccgag gttgccgtat tccatcaggt
    14161 aggtggtgcg gcaggccggc ggatcgtcgt tgtcgttgtg aaagatgtcg ccgaagctcg
    14221 tgactgtctg ctcgtagctg ttgcgaaaat tgaagccgag aatctcgacg ccggttccgt
    14281 ccacttgcat gcgcgcggag aagccaccca cgtacacgtg accatcgtca ctcttcgcgc
    14341 ccgcgtagct cagcggcgcc gatccgaaca ccgcctgggc gccaccgtcg ggcacggggt
    14401 tgtaccagct gccgacgcgg aatgtcttgc caccgcgatc cgtgaagttg gcgcccgcat
    14461 tcccctggct gaagtaccag cggccgtcgg ggccgaatgt gaccgagtgc agcgcgtggt
    14521 cgtgattgcg tccgtcgaaa cccgagagca acacttcgcg cttgtcgatg gccgggttga
    14581 agagcctgtc gccgtcgacg tcggtataaa caatgagatc gggggcgttg gagacaatca
    14641 ccttgttgcc gatcacggcg atgccgagcg gagccacgag cgtgggctcc tgcacgaacg
    14701 tcgtggactt gtcggcccgg ccgtcgcgat ccgtgtcttc gagcaccacc acccgatccc
    14761 ctttcggatc gcgctccttg tggcgccggt agttcacgcc ttcggtaacc cagatgcgcc
    14821 ccgcagcatc gatgtcgatg ttggtggggt tcttgagcat gggcgatcgc gcccaaagcg
    14881 tcacttcgag cccctcggga accgagaaga tgtcgagcgg cacgacagtc ggtgcgggcg
    14941 tggctgccgg cggatcggcc gcgacactga tcgttgcgca cgcagacagg caggcaagca
    15001 gaaccggctg ccgggcagtc agtagtttca tgagcagtcc cctgaaatga attggcctgg
    15061 aatggattgg ctcagaacga atcggccgcg acgtacgcgt cgatgagcgc ctgctcgcct
    15121 tccttgcccg gcttcgcatt gccgtgctcc atgccgaaaa ttccgttgaa gcctttcccg
    15181 ctgatgtggc ggaacaggtt ccggtagttc atctcgccgg tgcccggctc cttgcgcccc
    15241 gggttgtcgc cgatctggaa gtacgcgatc tcgctccacg cgagattgat gtgctggacg
    15301 atgtcgccct cgttgcgctg catgtgatac atgtcgtaaa ggatcttgca ggcgggacta
    15361 tctaccgcgc ggcagatggc ataggcctgg tccgaggtgc gcaggaacag atccggattg
    15421 tccgagagcg gctcgagcac catggtcagg ttgtgaggcg caaggatttc ggcgccggcg
    15481 cgcaacgtgt ccacgacgtg cccggtctgg atgccgatgg gcagcttgcg ttcgaaataa
    15541 cccggcacca cggtggccca gcgtgcgttc acccgtttgc ccacctcgac ggcggcgcgg
    15601 caggtatcga ggaatttctc ccggaactgc gcttcccccg tggtgagcga cgtcttccag
    15661 ttgtcaccgc cgtcgatgac gaacacaccc atggtcatgc cgaggcgtgc gagcgtctca
    15721 ccgatgcgct gctgcaactc cacgggccgt cccatgagtc cgttgtcctc gagcgcagtg
    15781 aacccttgcg cggccatgaa ttcgagttgg gcgatgggat cggggccagc gtgttccttg
    15841 aacatgccga agtgcggcgc gtagcgcagc ttgaactggc gggagccggg cttctttctg
    15901 gcggcggtgc tggcgcccgc gagggccgcc aacgaaacgg cggcggcgga ttgctgaaga
    15961 aacgtgcggc ggttgtccat ggttgggttc ctcggcggcg attgtaagcg actcgtaggg
    16021 cggcgtctga caagcgcagg cttcgccgcc gacagcccgc gcgcgtccgt cctgcgaacc
    16081 ttcgccggtg atcagcacca agcgtctcct cttcaacgcg gccaccttca tcccgggcgt
    16141 cgcgcagctt ccgcccgtaa agcgcgtctt caagcgtcat tcgatcggca ctcacggcac
    16201 gatcgaggcg cggtattgct attcggtatg gctgcggcac ctgatgtgcg cggcggcgaa
    16261 cgatctcgat acgaacccgc gcgcggttgc ggagctgggt ccgggcgatt ccatcggcat
    16321 cggcattgcg gccgtgctca ccggcgtgga gcgctacttc gcattcgacg tggtggcgca
    16381 tgccaacacg ccgcgcaacc ttgcgatctt cgacggtctc gtggacctgt ttctcgagca
    16441 gatcgatatt cccgatgctt ccgagtaccc gcgcgtgggg ccgacgctca aggattaccg
    16501 tttccccgga cacatattca cgccggatcg catgcgcgag gcgctcgatc cggcgcgcct
    16561 tgcgtatgtc cgcgaatcca tccagcgcac ggaccagaag gattcaatga tccagtaccg
    16621 tgcgccgtgg agttcgtcgg gtgcggtcga gcacgaaact ctggacctcg tgtactcgca
    16681 ggccacgctc gaacacgtcg atggcctgcc cgaggtgtat cgcgccatgt accagtggct
    16741 gaagcccggc ggctacatgt cgcaccagat cgacttcaag tgccacaact cagccagaga
    16801 atggaacggc cactggacct actcgaagct gatgtggaag ctggtgcgag gcaaggacgt
    16861 ctggctcatc aaccggctgc cctactccac acacgtggag ctcatggaga aagccggctt
    16921 ccgcatcgtc ggtgagcagc tcgtacgccg ggcatcgaat gtccggcgcc gccagctcgc
    16981 ctcacagttc cgctcgatac cggacagcga cctcacgacc accgacgctt tcgtccaggc
    17041 cgtgaaaacg gtgccaggca ccgattgcta gcaaacggtg cctgacccca tttacagcga
    17101 aatctcgaac ccgctcttta ccggcgcaat ggcgatgcga cgcgcggtga aacgctcgat
    17161 gaccgcgacg ttggaacgca gatgcggtgt ggcctgcgcc gtgacgaagc tccccccatc
    17221 cccgagcgcc atcggcagca gcagctgatc ggccaggtgc tcgcccacgg gcgccaccgc
    17281 cgatagatag tgtcgcgccg cttccgccgc ctgcccggcc acgatttcgg cgcgcacgcc
    17341 cttctccccg aagccggtga acacttcgat gacgttctcg tactcgagcg tgatggtgag
    17401 cgcgttaccc ggtcccacgt cgttcggcag accccgcaac agcagttgtt cccgcgacca
    17461 gcccagcgcg cggccgatga cctcgagctc acgctgcgcc acgtggatcg ggatcgcaga
    17521 cacataggcc tccgcgtagc cgcgcaccca cgcgccacgt tccagcaagt tgagcgcctt
    17581 gaggcgcgcc cccggcgtga tgcgcgcgcg gatttcgccg ccaccgcgcg gataaaatcc
    17641 ataactcgtg agatcgagct gcacgtccgc acccatgcga cctaacaacg gcaggaaact
    17701 tcgggccagg aagtcaaacg gtggtgaggc gcggttgtgc gtgccgccgg tgatgcgtac
    17761 gcggctcacg ccgcctgcgg tgagcaatgc cggcagcacc gtctggaata ccagcgtgca
    17821 acttcccgcg gtgccaatcg caaacgagta gtcgcccgcg gcgagcctgc ccggccggaa
    17881 cgtgagcgca cgcgccccga gctccgcgcc ttcgatttcc gcggcgcaga tctccgccgc
    17941 ggctttgacc gcggtgagat gctgacgcat gagcccgggc ttctcccggt tcgcacgaat
    18001 gttcgtgatg cgaaacggct gctgcgtgca aatggcgagc gaaagcgaag agcgcagaat
    18061 ctgtccgccg ccttcgcctt ccgatccatc gatctcgatc attcgttggg tgcgctcaac
    18121 tgcgcgcgat gaaacgtccg ttctccagga actcgcccac ctgccgcgca acgtccgccg
    18181 acagcagcat gcccatgtgg ctcactggca gcgtcagatg tgcactgtgg cccggcagcc
    18241 tggtctcgcc gacgccgacg gtgccgtctg aatcctcatc gaagcgcgcg aagaaacggc
    18301 cgagccccat cggccgcgta ccggcgatgc agccgagctc gcgatcgcag cgccattcgc
    18361 gagcgtcggc cggctggacc aactcctccg tgaccatgcg gccaatcatc gagcggaata
    18421 ccggcaggcg cccgacgcgc tcggctgtct tgctcctgac cgtgggtgaa ccgagaaata
    18481 cgacgcgtcc aggcggcaga tctttagcct gctcgaaata gcgatgcagc acgattcccc
    18541 ccaggctgtg gccgacgaaa tgcacccgat ccgcatcgat gcggttgaca aattgggcga
    18601 gcgccgcgag taccggctgc accgagctac ccaccgtgcg atacgtgaag ctgtggcagg
    18661 caatcccgtg tttcgccgcg atgcgccggc gcatgagcgc cgactcggcg cccgtcaacc
    18721 acagtccgtg tacgaatacc gccgcagttt tcatggtgcc gggtggggaa agtggggagt
    18781 cagctggtcg gcggcggcgt gcgcacgcga atgtgaacct cgcgcagctg cttctcgctg
    18841 atctccgtcg gcgcatccgt gagcggatcc gcggccgtct gagtcttcgg gaacgcgatg
    18901 acgtcgcgga tgctgtcggt gccggccatg tgcatgacca gccgatcgag gccgaacgcg
    18961 atgccgccat gcggcggcgc gccgaacttg agcgcgtcga gcaggaagcc gaacttgagc
    19021 tgcgcctctt ccgcgccgat acctaacaag tcgaacaccg ccgactgcat gtcctggcgg
    19081 aagatacgca ccgaaccgcc ggcgatttcg ctgccgttga gcacgaggtc gtaggctttc
    19141 gccaacgcgc ccgacggaaa ggccttgagc gcggcgacgt cgtcgtccac cggcgcggtg
    19201 aacggatgat gcatggcgac ccagcgcttc tcgtccgggt cgtagtcgaa catcgggaac
    19261 tcgaccaccc acagcggctt ccagccttcg ctggtgagct tctggtccac gcccaccttg
    19321 aggcgcaacg cgccgatggc atcgctgact acctttgccg aatcagcacc gaagaaaatg
    19381 aggtcgccat ctttcgcgcc cgtgcgatcg aggatgccct tcaccgattc atcggacagg
    19441 aatttcacga tgggcgactg caggccttcc ttgcccttcg cggtctcgtt gaccttgatg
    19501 tacgccagtc ccttcgcgcc gtaacgcgcg acgaaggcgg tgtactcgtc gatctgcgag
    19561 cgcggcatgg cgccgccgcc cggtacgcgc agcgcggcca cgcgaccctt cggatctttg
    19621 gcgggcccgg cgaacacctt gaagtcgctg ccggcgacga gatcggccac gtcgacgagc
    19681 tcgagcgggt tacgcagatc gggcttgtcg gaaccgaagc ggcgcatggc ttcggcgtac
    19741 gccatgcgcg ggaaaggctc ggccaggtcg acgccgatgc cgtccttgaa gaagtgacgc
    19801 gtcagcttct ccatgagctc catgatctga tcctgcgtca ggaacgaggt ctcgatgtcg
    19861 agctgcgtga attcgggctg gcgatccgcg cgcaggtctt cgtcgcggaa acacttcacg
    19921 atctggtagt agcggtcgaa gcccgcgacc atgagcagct gcttgaagat ctgcggcgac
    19981 tgcggcagcg cgaagaactt gccggcgtgc gtgcgtgacg gcaccaggta gtcgcgcgcg
    20041 ccttcgggcg ttgccttcgt gagcaccggc gtttcgatgt cgacgaagcc ggcgtcatcg
    20101 agatagtggc gcatggcgcg tgtgatcttg tggcgcaggc gcaggcgctt cgacatgtct
    20161 tcgcggcgca ggtcgatgta gcgatatttg aggcgcacct cttcgctcac gtgctcatcg
    20221 agctggaacg gcagcggctc ggcgcggttg agcgtcacga gttcgcgcgc cagcagctcc
    20281 accttgccgg aggtcaggtt ggcattggcg gtaccggcgg ggcgctcgcg caccaggccg
    20341 cgtacctgca ccacgaactc gttgcgcaac ttctcggcct cgttgaactg ggcctggttg
    20401 tccgggtcga ataccacctg aaggaggccc tcgcggtcgc gcacgtccac gaaaatgacg
    20461 ccgccatgat cgcggcggcg gtggacccag ccggcaaccg taacctcttg accgaccagc
    20521 ttttcgttga cctggccgca gtagtgagaa cgcatggaat tccgggaatg tgctgacgaa
    20581 agggcggcga tgttaacggt tcagggcaga gtcaggaaag ccgattcatc atggtgccgg
    20641 gtggggaaag cggggattgt gctcaatggg gtactccctt tactttcgcg cccggcaaac
    20701 acactccccg ctttccccac ccggcaccca ccaggctcaa acatgacccg aaaactcgtt
    20761 gcaatgctcg ccatcaccgt cgccggcctt gccgccgtcg cctccgccca ggcccaaact
    20821 tgggtggcag gcaagcacta caccgtcatc cccacccagc gcaccaacgt gcccgcgggc
    20881 aaggtcgagg tcatggaagt gttttcgtac ggctgcccgg cctgcaacag cttcctgccc
    20941 accatgaaga agctcaaggc ggccctgccg ccgaatgcac agctggttta cctgcccgct
    21001 tcatggaaca agtcggaaaa ctggccgctg ttccagcgcg cttatctcac cgcgcagtcg
    21061 ttaggtgtcg ccgagaaagc gcacgaagtc atgttccagg ccatctggag cacgggcgag
    21121 ctcggcgtca ccgaaccttc cggccgcctc aagaaagtcc tgccttccat cgaagacacc
    21181 gcgcgtttct acgaacggac cacgggcgtg aaggccgcga ctttcgtgaa tgcctcgaag
    21241 tccttcggcg tcgacctcaa gatgcgccag gccgacagcc agatcatcgc catgcaggtc
    21301 agcggcacgc cgacgctggt ggtgaacggc aaataccgcc tgaacaacga ctcgctcacc
    21361 acggaacaga tggtcgacct cgtcaagttc ctcgtggcca aggaaagcgc gccggcagcg
    21421 gcggcgcccg ccaagaaagg ctaggcgacc gcggcacggc ggcgcttcca caggcgccgc
    21481 cctaacc
//
LOCUS       DASQJS010000324         8578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2705761, whole genome shotgun sequence.
ACCESSION   DASQJS010000324 DASQJS010000000
VERSION     DASQJS010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2705761"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VEW08_13015"
     CDS             <1..727
                     /locus_tag="VEW08_13015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01184.1"
                     /note="This model describes the ATP binding subunits of
                     ATP-binding cassette (ABC) transporters for nitrate
                     transport, or for bicarbonate transport, in bacteria and
                     archaea; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate ABC transporter ATP-binding protein"
                     /protein_id="HYJ41705.1"
                     /translation="EGFQLDIKRGECVSLIGHSGCGKSTALSMCAGLTEVSDGGITLD
                     GREVTSAGPDRGVVFQAPNLLSWLTARQNVALGVDRVYPHASNAERSDIVDYYLTRVG
                     LADSLDKLSRDMSNGMRQRVGLARAFALSPKLLLLDEPFGMLDALTRWDLQEVLMDIL
                     RRTKITAICVTHDVDEAILVADRVIMMTNGPCARIGKVMQVDLPRPRTRKMLLEHPRY
                     YAYREELLSFLEEYEHGAGNRAA"
     gene            759..2027
                     /locus_tag="VEW08_13020"
     CDS             759..2027
                     /locus_tag="VEW08_13020"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HYJ41706.1"
                     /translation="MTMWKASGFAVLGMFLGSGAAHADGLDPVWGAFGEIKPIIDTRL
                     RMEDVDQDPLAEDASAITLRARLGFETGKAWNTSLLVEGEAVMPLDDDYRPDPAVPEM
                     VTYPVVADGEAYEINRFQFTNTSLPGTTLTLGRQRILLDDQRFIGNVGWRQNEQTFDA
                     FRMVNRSVTNLVLDATYLNRVNRVNGPDSPQGVYKGDGFLLNGGYQTKIGKLSAFGYL
                     LDFEPITGIPAGINPVRDSTSTYGVRFAGDKPAGKIKLAYAASYATQTDYGDNPLDFD
                     LDYVFGELTATFRQFGLGVGTEIMEGNGLAGTAGKGFTTPLATLHKFQGWADKFLTTP
                     PNGLEDLYATATVNLKGVGVLDTLGFIVSYHDYNAEQVSADYGSEWNASIAAKHKRFN
                     LMLKYADYQQGELVSARTTSKLWAQIEFIW"
     gene            2121..2942
                     /locus_tag="VEW08_13025"
     CDS             2121..2942
                     /locus_tag="VEW08_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HYJ41707.1"
                     /translation="MSYLAPAEFVTKMVDAGESKVFMSTRDTLIRAFMAGAILALAAV
                     FAVMVTQQTGYPIIGAILFPVGFCMLYLLGFDLVTGVFVLAPLALLDKRPGVTFGGVM
                     RNWGLVFCGNFAGAFTVAVLMSVVFTFGFTTDPNPIGQFIAHVGESRTIGYKEHGMGG
                     MVTLFVRAMLCNWMVSTGVVGAMISTSVGGKVLAMWMPIMVFFAMTFEHSVVNMFLFP
                     SGILMGANFNWADYFLWNEIPTILGNIVGGLAFTGLTLYSTHIKTAPKRNLKLAA"
     gene            3008..4729
                     /locus_tag="VEW08_13030"
     CDS             3008..4729
                     /locus_tag="VEW08_13030"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HYJ41708.1"
                     /translation="MPGQLKISLGQYSDKGRKESNQDFHGACIPGEPQLAEKGIAIAL
                     ADGISSSNVSQVASQSAVRVFLEDYYCTSPAWSVKKSAQAVLMAVNSWLYAQTQQSPY
                     RFEKDRGYVCTLSALVLKSNTAHLFHAGDARIYRLRDGLLEQLTNDHRVWVSSEQSYL
                     SRALGIHSHLDIDYQALRLEQGDVFVFATDGVHEFANSRFMINAVKDHGGDLDAAARL
                     IANEAFERGSQDNLTTVIVRVDTLPGQSAGEVHRQLTELPFPPDLAPRMVFDGFTIVR
                     ELHASSRSHVYLATHGETAAPVVIKTLATEMRADAGHLDRFLMEDWVAQRISNAHVVK
                     AAARGYKRNYLYTVTEFVDGQTLHQWLIDHPRPTVEAVRGIIEQIANGLLAFHRLEML
                     HQDLRPQNVMIDATGTVKLIDFGSTRVAGISEIDSPIERSEMLGTLQYTAPEYFLGES
                     GTARSDLFSLGVIAYQMLSGRLPFGAEVPKARTRAAQRQLKYQSVLDDEREIPAWIDE
                     VLRKATHPDPSRRYEELSEFVYDLRHPTERYLSRKRAPLLERKPVAFWQGVSMILAVA
                     VVILLLS"
     gene            4776..7016
                     /locus_tag="VEW08_13035"
     CDS             4776..7016
                     /locus_tag="VEW08_13035"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HYJ41709.1"
                     /translation="MMPTLKKKILIACLPLAWVAPAFSIEIDGRVGESEWQGSQHVTD
                     FRKTQPLTGEPPSHATEAWILSTPEGLAVAIRNTQPASVPRTQQRVQRDFEEQVDRIN
                     VIVDFDGDSRTGYNFRLSSTDDVYDGVVTDEKQFNKDWDGNWKHAVGVDETGWAVEVL
                     IPWYIAPMRDAKGDKRTMKIYLDRIIGSTGERVAWPQASLERSRFLSDFAPIDVTNYS
                     QSLFAMTPYVSGLYDNVGKSSDGDAGVDVVWKPNGQTQLTATLNPDFGQVESDDLVVN
                     FSATETFISDKRPFFTENQGLFEFTTPSDYSQLLYTRRVGGPADDGEGSGDITAALKL
                     NGSLGGTKYGVFAADEAESVGRTFSALRVVRDFATQNVGFMATNVKRPYLDREATVFG
                     VDHNWRPNARWNVRTRVFGSDIEQAGTGATDSGATVWADYEMDRGWRQQWIAMHFGNE
                     LQINDAGYLSRNSTNYLHWQVNRRFTDFPESSRYSSKDWRGRISTRYNDHGLLMNHQV
                     RVSRESRLRDGSYEYGEVNVNSAGFDDLITRGNGVLWLPPNFNGYFNYERPRKGNWSW
                     EVDVNVDSGGLAGNSKVGYNVNFEPTYFVSDAFSVYVGLYAGRIPDWLIWQSDNLIGS
                     FEEEELHFDAGLNWAIGSKQELRVKLQAIGLNGRLSQGYRVEPNGDATPSSEPIDDID
                     VKTLGFQIRYRYELAPLSYLYVVYGRGGFDREPSDEGAAGLLRDSFQLRDDEQLLVKF
                     SYRFEN"
     gene            complement(7021..8196)
                     /locus_tag="VEW08_13040"
     CDS             complement(7021..8196)
                     /locus_tag="VEW08_13040"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYJ41710.1"
                     /translation="MTFSKIMAATASLWLSACGGGGGENNNPAPLPTPTAPAPGVIGD
                     GRLPELLEWARASQNAPALAVVLVRRGQVAELAAVGRRSANNSIQVTTADRWQIGSMT
                     KAMTATLAAILVEDGLISWDTTCAEVWPELAGSIHAGFRNTTLRQLLSHSSGMKRDDP
                     FAPAEDSAPGTLMEKRRAWVEHLLTRAPEFPAGAYNYSNVGYVVAGAMLETRAATPWE
                     TALTNRVFTPLGMTHSGFGPPGTPGAIDEPLGHWSRTSGFEPVAPGSPDADNPLALGP
                     AGRVHTTLEDYARFMEAHIAGARGVSGLISAVSFRTLQTSVVGNYALGWNTQTFPTLG
                     VPGLSHGGSTGRWYSIVALAPSLDTGTMIVSNGGGDRAAAALLALDGLIARRIMASP"
BASE COUNT         1645 a         2683 c         2680 g         1570 t
ORIGIN      
        1 cgaaggattc cagctcgaca tcaagcgcgg cgaatgcgtt tcgctgatcg gtcactccgg
       61 atgtgggaag tccacggcct tgtcgatgtg cgccggcctc accgaggttt ccgacggcgg
      121 catcacgctc gatggacgcg aagtcacgag cgccggcccc gatcgcggcg tggtgttcca
      181 ggcgccgaac ctgctgtcct ggctcaccgc caggcagaac gtggcgctgg gcgtcgatcg
      241 cgtgtacccg cacgccagca atgccgagcg ttccgacatc gtcgactact acctcacgcg
      301 cgtcggcctc gccgactcgt tggacaagct ctcgcgagac atgtcgaacg gcatgcgtca
      361 gcgagtgggc cttgcgcgtg ctttcgcgct gtcgcccaag ctgctgctgc tcgacgaacc
      421 gttcggcatg ctggatgcat tgactcgctg ggacctgcag gaagtcctga tggacatcct
      481 caggcgcacg aagataaccg ccatctgcgt cacgcacgac gtggatgagg ccatactggt
      541 cgctgaccgc gtgatcatga tgaccaacgg cccgtgcgcg cgcatcggca aggtcatgca
      601 ggtcgatctg ccgcggccgc gcacccgcaa gatgctgctc gagcaccctc gctactacgc
      661 ctatagggaa gagctattga gctttctgga agagtacgag cacggcgcgg ggaaccgtgc
      721 tgcctaacaa aacaaaccgc aagcatgggg agtcttcgat gacgatgtgg aaagcttcag
      781 gatttgccgt tctcggcatg tttttgggtt caggcgcggc tcacgccgac ggactcgatc
      841 cggtctgggg ggcattcggg gaaatcaaac ccatcatcga tacgcgcctg cgcatggagg
      901 acgtcgacca ggatccgctc gcagaagacg ccagcgcgat cacactgcgc gcccggctcg
      961 ggttcgaaac cggaaaggcg tggaatacct cgctgctcgt cgagggcgaa gcggtcatgc
     1021 cgctcgacga tgactatcgg ccggatccgg ctgtcccgga aatggtgacg tatccggtgg
     1081 tcgcagatgg cgaggcctac gagatcaacc gctttcagtt caccaacacg agcctgccgg
     1141 gcaccacgct gacgctcggc cggcaacgca tcctgctcga cgaccagcgt ttcatcggca
     1201 acgtgggctg gcggcagaac gagcagacgt tcgatgcatt ccgcatggtg aaccgcagcg
     1261 tcacgaacct ggtcctcgac gccacgtacc tgaaccgcgt caatcgtgtg aacggcccgg
     1321 attcgccgca aggcgtgtac aagggcgacg gcttcctgct gaacggcgga taccagacga
     1381 agatcggcaa gctcagcgcg tttggctatc tactcgactt cgagccgatc accggcattc
     1441 cggccggcat caaccccgtt cgcgattcca cgtcgacgta tggagtgcga ttcgcggggg
     1501 acaagcctgc gggcaagatc aagctcgcct atgcggcgtc ttacgccacg cagaccgact
     1561 acggcgacaa cccgctcgat ttcgacctcg actatgtttt cggcgagctc accgccacct
     1621 tcaggcagtt tggtttgggg gtcggcacgg agatcatgga aggcaacggc ctggccggta
     1681 cggcaggcaa aggcttcacc acaccgctcg ccacgctgca caaattccag ggctgggccg
     1741 acaagttcct gacgacaccg ccgaacggcc tcgaagatct gtacgcaacc gccaccgtca
     1801 atctcaaagg cgtgggcgtg ctcgatacgc ttggcttcat cgtgagttat cacgactaca
     1861 acgccgagca ggtctccgcc gactacggca gcgaatggaa cgcctccatc gccgcgaaac
     1921 acaagcgttt caacctgatg ctcaagtacg cggactacca gcagggagag ctggtgtcgg
     1981 cgcgcaccac cagcaagttg tgggcacaga tcgaattcat ttggtaaccg taaaccggtg
     2041 ccgggccggg aaaccgggat aactcaatga gttttcccgg tttcccggcc cggcaccact
     2101 aactagaggg gaggagatcg atgtcatatt tagcaccagc ggagttcgtc accaagatgg
     2161 tggacgccgg cgaatcgaag gttttcatgt cgacgcgcga cacgctcatt cgcgcgttca
     2221 tggccggcgc cattctcgcg ctcgcggccg tgttcgccgt gatggtgacg cagcagactg
     2281 gctacccgat catcggcgcc atcctgttcc cggtgggctt ctgcatgctc tacctgctgg
     2341 gcttcgacct ggtgacgggc gtgttcgtgc tggcgccgct ggcgctgctc gacaagcgtc
     2401 ccggcgtgac cttcggtggc gtgatgcgca actggggcct ggtgttctgc ggcaacttcg
     2461 ctggtgcttt caccgtcgcg gtgctgatgt ccgtcgtttt cactttcggg ttcacgactg
     2521 atccgaaccc catcgggcag ttcatcgcgc atgtcggtga gtcacgcacg atcggctaca
     2581 aggaacacgg catgggcggc atggtcacgc tgttcgtccg cgccatgctc tgcaactgga
     2641 tggtgtcgac cggtgtcgta ggcgcgatga tctccacctc ggtgggcggc aaggtgctcg
     2701 ccatgtggat gccgatcatg gtgttcttcg cgatgacctt cgagcactcg gtggtgaaca
     2761 tgttcctgtt cccctcgggc atcctcatgg gagccaactt caactgggcg gactacttcc
     2821 tctggaatga gattccgacc atcttgggca acatcgttgg tggcctcgcg ttcaccggcc
     2881 ttaccttgta ttccacgcat atcaagaccg cgccgaaacg caatctgaag ctcgcggcct
     2941 gaagccgggt tgaaaaagaa ccccgggcct cgcgtggccc ggggttccct atacactcgc
     3001 gtgatccatg cccggccagc tgaaaatctc gttaggccag tattccgaca agggccgcaa
     3061 ggaatccaac caggatttcc acggtgcctg tattcctggc gagccgcagc tcgcggaaaa
     3121 aggcatcgcc atcgcgctcg ccgatggcat cagcagcagc aatgtcagcc aggtagccag
     3181 ccagtccgcg gtgcgagtct ttctcgagga ctattactgc acctcgcccg cgtggtcggt
     3241 gaagaagtcc gcgcaggcgg tgctgatggc cgtgaattcc tggctgtatg cgcagaccca
     3301 gcaaagcccc tatcgctttg aaaaggaccg cgggtacgtc tgcacgttga gcgcgctcgt
     3361 gctcaagtcc aataccgcac atctgtttca cgccggcgat gcccgcatct accggttgcg
     3421 cgatgggctg ctcgagcagc tgacaaacga tcaccgggtc tgggtgtcca gcgagcagag
     3481 ttatctcagc cgcgcgctcg gcattcactc gcacctcgac atcgactacc aggccctgcg
     3541 gctggagcag ggtgacgtct tcgtgttcgc caccgacggc gtgcacgagt tcgcgaactc
     3601 gcgtttcatg atcaatgcgg tgaaggatca cggcggtgac ctggacgccg cggcgcgttt
     3661 gatcgcgaac gaagccttcg agcgaggcag ccaggacaac ctcaccacgg tcatcgtgcg
     3721 tgtcgacacc ctgcccgggc agagcgccgg ggaagtccat cggcagctca cggaactccc
     3781 gtttccgcca gatcttgcgc cgcgcatggt tttcgatggt ttcaccatcg tgcgcgagct
     3841 gcatgcgagc agtcgcagtc acgtctatct ggccacccat ggtgaaaccg ctgctccagt
     3901 ggtcatcaag acgctggcga ccgaaatgcg tgcggatgcc gggcacctcg accggttcct
     3961 catggaagac tgggtggcgc aacgcatcag caatgcgcac gtggtcaagg cggcggcgcg
     4021 cggctacaaa cgcaactatc tttacaccgt gaccgagttc gtcgatgggc agaccctgca
     4081 tcaatggctc atcgaccacc cgaggcccac ggtcgaggcg gtgcgcggaa tcatcgagca
     4141 gatagccaat gggctgctgg cgttccaccg cctggagatg ctgcaccagg acctgcggcc
     4201 gcagaacgtg atgatcgacg ccaccggcac ggtgaagctc atcgacttcg gctcgacgcg
     4261 agtggccgga atctccgaga tcgattcgcc gatcgagcgc agcgagatgt tgggcacgct
     4321 gcaatacacc gcgcccgaat acttccttgg cgagagcggg accgcgcgct cggacctgtt
     4381 ttccctgggc gtcatcgcct atcagatgtt gtcgggtcgg ctgccctttg gcgccgaggt
     4441 tcccaaggca cgcacccgcg ccgcgcagcg ccagttgaaa tatcaatccg tgctcgacga
     4501 cgagcgcgag attccggcgt ggatcgacga ggtattacgc aaggccaccc atccggatcc
     4561 gagccggcgc tacgaagaat tgtcggaatt cgtctacgac ctgcgccacc cgaccgagcg
     4621 ttacctcagc cgcaagcgcg cgccgctgct cgagcgcaaa cccgtggcat tctggcaggg
     4681 cgtatcgatg atcctcgcgg tggccgtggt gattctgctg ctgagctagc tctccccggt
     4741 gccctaaacc tttttcgggc ccgtcgcgtc ttagaatgat gccgacgctc aagaagaaaa
     4801 ttctcatcgc gtgcttgccg ctcgcatggg tcgcgcccgc tttctccatc gaaatcgacg
     4861 gacgcgtcgg tgaatccgaa tggcagggtt cgcagcacgt caccgacttc cgaaagaccc
     4921 agcccctcac tggtgagcct ccctcgcatg cgacggaggc gtggatcctg tcgacgcccg
     4981 agggcctggc cgtggcgatt cgaaacactc agcccgcatc ggtgccgcgc acccagcagc
     5041 gcgtgcagcg ggatttcgaa gagcaggtcg atcgcatcaa tgtcatcgtc gatttcgatg
     5101 gcgacagccg taccggatac aacttccgcc tgagctcgac cgatgacgtc tacgacggcg
     5161 tggtcaccga cgaaaaacag ttcaacaagg actgggacgg aaactggaag cacgcggtcg
     5221 gcgtggacga gacgggttgg gcggtcgagg tgctgattcc ctggtacatc gcacccatgc
     5281 gtgacgcgaa gggcgacaag cgcacgatga agatctatct agaccgcatc attggctcga
     5341 ccggcgagcg cgtcgcctgg ccgcaggcga gcctggaacg gtcgcgcttt ctgtccgatt
     5401 tcgcgccgat cgacgttacc aactacagcc aatcgctgtt cgcgatgacg ccctacgttt
     5461 cgggcctcta cgacaacgtg ggcaagagca gtgatggcga cgccggcgtc gacgtggtct
     5521 ggaagccgaa cggccagaca cagctgacgg ccacgttgaa tccggatttt ggccaggtgg
     5581 agagcgacga cctggtggtg aacttcagcg ccaccgaaac cttcatcagc gacaagcggc
     5641 cgttcttcac cgaaaaccag gggctgttcg aattcaccac gccttctgat tacagccagc
     5701 tgctgtacac gcgccgcgtg ggcggacccg ccgacgacgg cgagggttcg ggcgacatta
     5761 ccgccgcgct caagctcaat ggcagcctgg gtggaacgaa gtacggcgtg tttgcggccg
     5821 atgaggccga gtccgtcggc cgcactttca gcgcgctgcg tgtggtgcgg gatttcgcca
     5881 cgcagaacgt cggtttcatg gcgaccaacg tcaagcgccc ttacctcgat cgcgaggcca
     5941 cggtgttcgg cgtcgatcac aactggcggc ccaacgcacg ctggaacgtg cgcacgcgcg
     6001 tgttcggcag cgacatcgag caggccggaa ccggcgccac cgattcgggc gcgacggtct
     6061 gggccgacta cgagatggac cgcggctggc gccagcagtg gatcgcgatg catttcggca
     6121 acgaactgca gatcaacgac gcgggttatc tgtcgcgcaa cagcactaac taccttcact
     6181 ggcaggtgaa tcggcgcttc accgacttcc ccgaatcttc gcgctattcg tcgaaggact
     6241 ggcgcgggcg catcagcacc cgctacaacg atcacgggct gctcatgaat caccaggtcc
     6301 gcgtgagccg tgagagccgt ctgcgcgacg ggtcttatga atacggcgag gtcaacgtca
     6361 acagtgccgg tttcgacgac ctgatcacgc gcggcaatgg cgtgctctgg ctcccgccca
     6421 atttcaacgg ctatttcaac tatgaacggc cgcgcaaggg gaactggtcc tgggaagtcg
     6481 acgtcaacgt ggacagtggc ggcctcgccg gcaacagcaa ggtgggctac aacgtgaatt
     6541 tcgagcccac ctacttcgtg agcgatgcgt tcagcgttta tgtcgggctg tacgccggcc
     6601 ggattccgga ctggctgatc tggcagagcg acaacctcat cggcagcttc gaagaagaag
     6661 aactgcactt cgacgccggg ctcaactggg ccattggcag caaacaggaa ttgcgagtga
     6721 agctacaggc catcggcctg aatgggcggt tgagccaggg ataccgcgtc gagccgaatg
     6781 gcgacgccac acccagcagt gagccgatcg acgacatcga cgtgaaaacg ctcggcttcc
     6841 agatccgata ccgctacgag ctcgcgccgc tttcctatct atatgtggtg tatggccgtg
     6901 gcggcttcga ccgggaaccg agcgacgagg gagccgcggg cctgttgcgc gacagcttcc
     6961 agctgcgcga tgacgagcag ctgctggtga agttcagcta ccggttcgag aactagcggg
     7021 tcagggcgac gccatgatgc gtctggcgat cagtccatcg agtgccaaca atgcggccgc
     7081 cgcgcgatcg ccgccgccat tgctgacgat catggtgccc gtatccaacg acggcgccaa
     7141 cgccacgata gagtaccaac ggcccgtgct gccgccgtgg ctcaaacctg gcacacccag
     7201 tgtaggaaac gtttgagtgt tccagccgag cgcgtaattg ccgacgacgc tggtctgcag
     7261 agtccggaag gacacggcgc tgatcaaccc ggagacgccg cgggcacctg cgatgtgcgc
     7321 ttccatgaag cgcgcgtagt cctcgagcgt cgtgtgcact ctgccggccg gcccgagagc
     7381 gagcgggttg tcggcgtccg gacttcctgg ggccaccggc tcaaatcctg acgtgcggct
     7441 ccaatggcca agcggctcat cgatcgcgcc cggcgtgccg ggagggccga aaccgctatg
     7501 cgtcattccg agcggcgtga atacgcggtt tgtgagggcg gtttcccatg gcgtagcagc
     7561 gcgtgtctca agcatcgcgc ccgccacgac gtaccccacg ttcgagtagt tataggcgcc
     7621 cgcaggaaac tcgggagcgc gggtcagcag atgttcgacc caagcgcggc gtttctccat
     7681 caacgtgccg ggggcggagt cctcggccgg ggcaaacggg tcgtcccgct tcatgccaga
     7741 gctgtgggag agcagttgcc gcagcgtcgt gttccgaaac cctgcgtgaa tgctgcccgc
     7801 gagttccggc cacacttcgg cgcaagtcgt gtcccagctg atcaagccgt cttcgaccag
     7861 gatcgccgcc aacgtagcgg tcattgcttt cgtcattgag ccgatctgcc agcgatctgc
     7921 cgtcgtcacc tggatgctgt tgttagccga gcgccggccg acggcggcaa gttcggcgac
     7981 ctgacctcgg cgcaccagaa ccaccgcgag tgcaggggcg ttctgcgatg cgcgggccca
     8041 ctcgaggagc tcgggaagtc ggccgtcgcc gatgacccca ggagcaggtg cggtgggggt
     8101 gggcaatggc gcggggttgt tgttctcgcc gccaccgccg ccacaggccg aaagccacaa
     8161 ggaagccgtg gcagccatga ttttcgagaa ggtcatgcgg atccccgggc ggtgtgcgct
     8221 cttatataga tgagtcgtcc ggggatggac cgttcccggc cagacaggtt ccggtaatgg
     8281 cacgccaacc caatgatata taacgaaaag ggtaatactg gccggggaag ccccgagtgg
     8341 gctccgggcg acctcggaag gacagctcac atgggcttgc ggcgcctgag gtcgctgcat
     8401 atactgcgcg ccctttcgcc tgggaccaaa gtctcaagcg attgatttca agcgaaatcg
     8461 ggcgaggttg ggcgagcctt cgaagctcca aacccaagac cactcgagca tcgcggcgca
     8521 aattcttgct gccgcgcttc tccagtgttc cctcgttaga cctagctaca ccgcgggc
//
LOCUS       DASQJS010000325        10539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1767956, whole genome shotgun sequence.
ACCESSION   DASQJS010000325 DASQJS010000000
VERSION     DASQJS010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10539
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767956"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VEW08_13045"
     CDS             complement(<1..405)
                     /locus_tag="VEW08_13045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HYJ41711.1"
                     /translation="MDVDNKNDKQSPGEVTRLLQSWADGDKSALEALWPMVYDDVRRL
                     ARRQMAGERGDHTLQSTALVNEAFIRLAGQRVFEWQNREQFLSLAAQIMRRVLVDYAR
                     KRGAQRRGDGAKKLSLHDTQAAIEVDEAQALNS"
     gene            589..2418
                     /gene="typA"
                     /locus_tag="VEW08_13050"
     CDS             589..2418
                     /gene="typA"
                     /locus_tag="VEW08_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HYJ41712.1"
                     /translation="MANLPIRNIAIIAHVDHGKTTLVDCLLRQSGTFAAHEKIAERVM
                     DSNDIEKERGITILAKNCAIQYGGTRINIVDTPGHADFGGEVERVLSMVDTVLLVVDA
                     VEGPMPQTRFVTRKALALGLNAIVVVNKIDRPGARPDWVVDQTFELFDKLGATDRQLD
                     FPVIYCSALEGWASMDHTKREPDMRAMYEAILHHVPAPKSEPDAPLQLQISTLDYNSY
                     VGRIGIGRIRRGTVKTGQTIQLWNGTEDHGTAKILQVLTFKGLSRESVDSAVAGDIVA
                     VTGIEDVHIGYTINEVDKPDALPPISVDEPTLVMNFQVNTSPLAGKEGKFVTSRQIRE
                     RLMRELESNVALRVDMTADADVFRVSGRGELHLTILIENMRREGYELAVGKPRVLTKT
                     IDGVVHEPFEALTVDIDENHQGAVMEALGRRKGELKDMANDGRGRVRIEYRVSARGLI
                     GFQSEFMNATRGTGLQSHIFDGYAPLAGDIPDRHNGVLISSEPGFAVAYSIFALQERG
                     RMFVSHGEAVYEGMIIGIHSRENDLVVNPIKTKKLTNVRAAGKDDALILTPPIDLTLE
                     YAVEFIADDELVEVTPKSIRIRKRKLLEHERKRASRVDEALLA"
     gene            2564..2851
                     /locus_tag="VEW08_13055"
     CDS             2564..2851
                     /locus_tag="VEW08_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41713.1"
                     /translation="MKKPSSKWHAISIVVQESSCATAAMCRNKRFLSAQVPMLPMRDC
                     DRAAQCACKFKHFDDRRSGVRRTGDMHNDVRSQFLEHNRRATRGRRTVDSR"
     gene            complement(2862..3584)
                     /locus_tag="VEW08_13060"
     CDS             complement(2862..3584)
                     /locus_tag="VEW08_13060"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HYJ41714.1"
                     /translation="MLRTIIFSVALIIAASVLGYQVKQVGGGRESISVKGLAEKPIKA
                     DRAEWTIRLQVKGATIAEALGKLRKERPALDQFLGKAGFEAASLTESNEASEPNYEQV
                     ESPNGNMRSVQDGHIARQSITINTADIGKIIAAARGIVQFEADGHPVSFGEPQFLVSN
                     LEDVKMSLIGAAMQNSRARAQEFAKNGGVEVGAMRSASQGAFYILPASANADVSDWGG
                     TYDKTTVDKIARVVVTVDYGIK"
     gene            3668..4126
                     /locus_tag="VEW08_13065"
     CDS             3668..4126
                     /locus_tag="VEW08_13065"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HYJ41715.1"
                     /translation="MTGVDLQTENVANIIQLALGPVFLLSGVGITLSMLTQRLSRIVD
                     RARTLEDRREATANEAKLKQIDRDLRVILRRARYINSAIGLSTTSALLTALVVTLLFA
                     SAFTPMSVGGVIAVMFVASMVCLSTAFLMFLIEVRIATNTLRIGVQKQDK"
     gene            4126..5037
                     /locus_tag="VEW08_13070"
     CDS             4126..5037
                     /locus_tag="VEW08_13070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYJ41716.1"
                     /translation="MRRASLASVCLAAAGAAFAIGPSDREKPPTTTQVMESAKPEDWR
                     RPDPANTLYMELSGGRVIIELAPRFAPQHVTNIKQLVAEKFFDGLGVVRVQDNFVAQW
                     GDADKTRPTESALRKLPDENAFAFSKRLPFVKLPDVDGYARETGWIDGFPVGVERKPS
                     GQAWMTHCYGVVGVGREDWPDTGSGAELYVVIGQAPRQLDRNIVTVGRVLRGIELLSS
                     LPRGTGGGLGMYEKPEQRAPIKSIRLAVDVPDAERTAIEVLRTDTPLFARYVESRRNR
                     RDSWYIVPAGHTEVCSINIPVRDVPAR"
     gene            5050..5772
                     /locus_tag="VEW08_13075"
     CDS             5050..5772
                     /locus_tag="VEW08_13075"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYJ41717.1"
                     /translation="MGRIFEVRKHTMFARWDRMAKQFARIAKDINMAVKAGGADPNSN
                     PALRRAVQNSRNLNMPKDKVEAAIKRAAGKDATAYEQIIYEGYAPHGVAVIVEAATDN
                     PTRTVAQVRAIFSKGSGNLGTTGSVAFQFRKMGVFRLNPAGIDHDDLELYLIDHGLEE
                     MGDSVGDKGEPQIVARCLFPEFGHMQEALEKRGIHPLIATHEYICTTPVELPEAQATE
                     VLELIDTLEQNDDVQNVYHSLV"
     gene            5775..6338
                     /locus_tag="VEW08_13080"
     CDS             5775..6338
                     /locus_tag="VEW08_13080"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HYJ41718.1"
                     /translation="MEAIEALNTRASAKTFGDIAPTKDHLATVLQAAVRAPDHGRLRP
                     WRFMLIEGDQRRKFGEMLAASALRRVPGLSDGDLQRERGKALRAPLIIVVACRIVPGT
                     KIPAIEQILAAGAAAQNILLALHTLGYVAAWKTGEAAYDTEVKKSLGFAADDHLVGFI
                     YTGGGAGATFAAGKAASVQDAMLAFPG"
     gene            complement(6308..7213)
                     /locus_tag="VEW08_13085"
     CDS             complement(6308..7213)
                     /locus_tag="VEW08_13085"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HYJ41719.1"
                     /translation="MPFLRSQDLYPAEPARGWIPWGALAPFLALVFVIATALPGDMLI
                     SKFVNISPEGNPLDPAALVAFTLVPFGFLLLLLLAWVRFVERRSLASIGIRGEHKLRE
                     FLRGHAFGLVGLFGIVMIIWMAGGLELTAVAPAFSQPASLVPVVLLLFTFALQSSVEE
                     LLFRGWLMSVLTKKFNVPIAVVVSSALFSLLHFSPRAHWLVSVGTFLFGLFACAWVLR
                     TRSVLGIMGWHAGWNWLLAIGFGLPLTGLDVGIPALLLDLKPVGADWLTGGPQGPEAS
                     VVCFGYFVLGIALLRFQPGKASIAS"
     gene            complement(7226..9112)
                     /locus_tag="VEW08_13090"
     CDS             complement(7226..9112)
                     /locus_tag="VEW08_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HYJ41720.1"
                     /translation="MFAKLMIANRGEIACRIARTARRLGIRTVAVYSDADAKAAHVAA
                     CDEAVHIGGSRPQESYLRGEVILAAARKMGADAIHPGYGFLSENAEFATACEREGVVF
                     VGPTPAAIAAMGSKSAAKALMKRAGVPLTPGYHGDRQEPDYLLQQAEQIGFPVLIKAV
                     SGGGGKGMRRVDRAADFAAALTSCQREASSSFKDGRVLVEKYLSAARHIEVQVFGDNH
                     GNTVYLFERDCSVQRRHQKVIEEAPAPGMTPERRRQMGEAAVAAARAVHYRGAGTVEF
                     IAADDAFYFMEMNTRLQVEHPVTEMITGLDLVEWQLRVAAGEPLPRKQSELAISGHAI
                     EARVYAEDPSRDFMPSIGKLAHVAMPAATADVRIDTGVRQGDEITPFYDPMIAKLIVH
                     GETRAEAIARMRMALAQYQIVGVRTNVEFLGRLMSAPAFVDAALDTALIEREREHLFA
                     PRGETSREMWELAAQAHWLAERRKATLLQARSPWDGRDAWALGVRGERRLKLREGEIE
                     REVAGSFSGSEAPGTFVVGNEIHLFNGGEHRVFEWIDPYLPVSEHADAHGGLLAIMPG
                     RVLAVHVKAGDEVAKGAPLVVLEAMKMEQTITAPAAGRIARVLCAPGDQVREGAELLA
                     FDDP"
     gene            complement(9120..9914)
                     /locus_tag="VEW08_13095"
     CDS             complement(9120..9914)
                     /locus_tag="VEW08_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYJ41721.1"
                     /translation="MTNYQHIEVDQRGAVRWLWLNRPEVRNAFNDTLVAEVAAAFAEV
                     EASVETRVVVVTSRGPAFCAGADLNWMRSMSTFSHADNHADALKVARMFHAVHSCSRP
                     VIGRVQGDAFGGGVGLAAACDIVVAAEAAHFALSEVKLGIVAATISPHLVRAMGARQA
                     ARYMLTGEKFTASQARTLGLVHEVTLMDGLDREVERIAHHLLAASPAALAATKRLLAD
                     VVETPMDDVLLAATAKCIADARVSPEGREGIAAFLEKRAPSWAPKK"
     gene            complement(9928..>10539)
                     /locus_tag="VEW08_13100"
     CDS             complement(9928..>10539)
                     /locus_tag="VEW08_13100"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYJ41722.1"
                     /translation="RIHGMPVGILANNGILFSESAQKGAHFIELCCQRRIPLVFLQNI
                     TGFMVGKKYENEGIARHGAKMVTAVACARVPKFTVIVGGSFGAGNYGMCGRAYAPRFL
                     WTWPNSRISVMGGEQAANVLATVRRDAIEGKGGTWSSDEEAAFKTPIREQYEAQGNPY
                     YATARLWDDGVIDPVDTRMCLALGLSVALRQPVPETRFGVFRM"
BASE COUNT         1951 a         3542 c         3239 g         1807 t
ORIGIN      
        1 ggaattcagt gcctgggcct cgtcgacctc gatggccgcc tgcgtgtcgt gcaggctcag
       61 tttctttgcg ccgtcgccgc gccgctgcgc gccgcgtttc cgggcatagt ccacgagtac
      121 gcgccgcatg atctgcgcgg ccagcgacag gaattgctcg cggttctgcc attcgaacac
      181 gcgctggccg gccagccgga tgaaggcctc gttcacgagc gccgtgctct gcagcgtgtg
      241 atcgcctcgt tcccccgcca tctgccgtcg ggccagtcgc cgcacgtcgt catagaccat
      301 gggccatagc gcctcgagcg ccgacttgtc gccgtcggcc caactctgca gcagacgggt
      361 gacctcgccg ggagactgtt tatcgttctt gttatcgacg tccacgtggc attgtggctg
      421 gagaaacggc cacatttctc gtttatggcg ccgaaatgag gaatgccccc attacccttt
      481 ccaggcctgc ggaccggtga ctttggcgtt taaccgccct tgtggtaact tgcgcgcccc
      541 ttggcccctg cctgcgcgct ccgtccttcg cgtaagccca tgagttctat ggcaaatctt
      601 ccaattcgaa acattgcgat catcgcgcac gtcgaccatg gcaagaccac gctggtcgac
      661 tgcctgctgc gtcagtccgg caccttcgcc gcgcacgaga aaatcgccga gcgagtcatg
      721 gactcgaacg acatcgaaaa ggaacgcggc atcacgatcc tcgccaagaa ctgcgccatc
      781 cagtacggcg gcacgcgaat caacatcgtc gacacgcccg ggcacgccga cttcggcggc
      841 gaagtggaac gcgtgctctc gatggtcgac acggtgttgc tggtcgtcga tgccgtcgaa
      901 ggcccgatgc cgcagacgcg tttcgtcacg cgcaaggcgc tggcgctcgg cctcaatgcc
      961 atcgtcgtcg tcaacaagat cgaccgtccg ggcgcgcgcc ctgactgggt cgtcgaccag
     1021 actttcgaac tgttcgacaa gctgggcgcc accgatcgcc agctcgattt cccggtcatc
     1081 tactgctcgg cgctcgaagg ctgggccagc atggatcaca ccaagcgcga gccggacatg
     1141 cgcgcgatgt acgaagcaat cctgcaccac gtgcctgcgc cgaaaagcga gcccgacgca
     1201 ccgctgcagc tgcagatctc gacgctcgac tacaactcgt acgtcggacg catcggcatc
     1261 ggccgcatcc gtcgcggtac ggtgaagacc ggccagacga tccagctgtg gaacggcacc
     1321 gaagatcacg gcacggccaa gattctgcag gtgctgacct tcaagggcct gtcacgcgag
     1381 tcggtcgact cggccgtggc cggcgacatc gttgccgtca cgggcatcga ggatgtccac
     1441 atcggttaca cgatcaacga ggtcgacaag cccgacgcat tgccgccgat ctcggtcgac
     1501 gagcccacgc tcgtcatgaa cttccaggtg aacacctcgc cgctcgccgg caaggaaggc
     1561 aagttcgtca ccagccgcca gatccgcgag cggctgatgc gcgagctcga gagcaatgtc
     1621 gcgctgcgcg tcgatatgac cgccgacgcc gacgtgttcc gcgtctcggg ccgtggcgaa
     1681 ctgcacctga ccatcctcat cgagaacatg cgccgcgaag gttacgagct cgccgtgggt
     1741 aaaccccgcg tgctcacgaa gaccatcgac ggcgtcgtgc acgagccctt cgaagcgctg
     1801 accgtggaca tcgacgagaa ccaccagggc gctgtcatgg aggccctcgg ccgccgcaag
     1861 ggcgagctca aggacatggc taacgacggc cgtggccgcg tgcgcatcga atatcgcgtc
     1921 tcggctcgcg gcttgatcgg cttccagagc gagttcatga acgcgacccg cggcacgggc
     1981 ctgcagagcc atattttcga cggctacgcg ccgctggccg gtgacattcc cgaccgccac
     2041 aacggcgtgt tgatcagcag cgaacccggc tttgccgtgg cgtactcgat cttcgcgctg
     2101 caggaacgcg gccgcatgtt cgtctcgcac ggcgaggcgg tgtatgaagg catgatcatc
     2161 ggcatccatt cgcgcgagaa cgatctcgtc gtgaacccga tcaagaccaa gaagctcacc
     2221 aacgtccgcg ccgccggcaa ggatgacgcc ctgatcctca cgccgcccat cgatctcacg
     2281 ctcgaatatg cggtggagtt cattgccgac gacgaactgg tggaagtgac gccgaagagc
     2341 atccgcatcc gcaagcgcaa gctgctcgag catgaaagga agcgcgcgag ccgcgtggac
     2401 gaggcgctgc tggcctgatt ccttcgggcg tttgtcatat gcggcgcgcg taaacacgcc
     2461 gctgtgacgc gactcccgga atgcgctcat cgtcccggac gctgagtatc cactttcgat
     2521 tgcggcacct accctgactt caacaagtac aggggaaaga ttcatgaaga agccgtcttc
     2581 gaagtggcac gcaatcagca tcgtggttca ggaatcgagt tgcgcaaccg ctgcgatgtg
     2641 caggaacaag cgcttcctgt cggcgcaggt ccccatgctg ccgatgcgcg attgcgaccg
     2701 cgcagcccaa tgcgcctgca agttcaaaca ctttgacgac cgccgtagcg gcgtgcgccg
     2761 taccggcgat atgcacaacg acgtgagatc gcaattcctc gagcacaacc gcagggcgac
     2821 gcgcggccgc cgcacggtag atagccgcta gcgcaatccc gctacttgat gccgtagtcc
     2881 acggtgacga cgacgcgcgc aatcttgtcg accgtggtct tgtcgtacgt gccgccccag
     2941 tcgctgacat ccgcgttggc gctggccggc aggatgtaaa acgcgccctg cgacgccgag
     3001 cgcatcgcgc ccacctcgac accgccgttt ttcgcgaact cctgggcgcg cgcccgtgaa
     3061 ttctgcatgg ccgcgccgat cagcgacatc ttcacgtctt cgaggttcga caccaggaac
     3121 tgcggttcac cgaaggacac cggatgtccg tcggcctcga actgcacgat gcccctggcc
     3181 gctgcgatga tcttgccaat gtccgcggtg ttgatggtga tcgactggcg cgcgatgtgc
     3241 ccatcctgca ctgaacgcat gttgccgttg gggctttcca cctgttcgta gttaggttcg
     3301 ctcgcctcgt tggattcggt cagcgacgcc gcttcgaacc cggccttgcc caggaactgg
     3361 tcgagcgccg gccgttcctt gcgcagcttg cccagcgcct cggcaatcgt cgcgcccttg
     3421 acctggagac ggatggtcca ttccgcgcga tctgccttga tgggcttctc cgccaggccc
     3481 ttgaccgaga tggactcgcg accgccgccg acctgcttga cctggtagcc caggaccgac
     3541 gccgcgatga tgagcgcgac cgagaagatg atggttctga gcatggacgc cctctttatt
     3601 agtgcccgac atccgtgcga cagcgtcgcc gatgacaagt tcagggattg tggcagactt
     3661 cgcccccatg actggcgtcg atctgcaaac tgaaaacgtc gcaaacatca tccagctggc
     3721 gctgggcccg gtgttcctgc tctcgggcgt cggcatcacg ctgagtatgc tcacccagcg
     3781 gctgtcgcgc atcgtcgacc gtgcgcgcac gctcgaagac cggcgtgagg caactgccaa
     3841 cgaagcaaag ctcaagcaga tcgaccggga cctgcgcgtc atcctgcgcc gcgcgcgtta
     3901 catcaacagt gccatcgggc tgtccaccac ctcggcactg ctcaccgccc tggtggtgac
     3961 tctgctgttc gccagcgcat tcacgcccat gagcgtcggc ggcgtcatcg ccgtcatgtt
     4021 cgttgcctcg atggtgtgtc tgtcgacggc gtttctcatg tttctcatcg aggtgcgcat
     4081 cgccaccaat acgctgcgca tcggcgttca gaagcaggac aagtgatgcg ccgggcctca
     4141 ctcgcttcgg tctgtcttgc cgctgccggc gccgccttcg ccatcggtcc gagcgatcgg
     4201 gaaaagccgc ccaccacgac ccaggtcatg gagtccgcga agccggagga ctggcggcgg
     4261 cccgaccctg ccaacactct ttacatggag ctttcgggcg ggcgggtcat catcgagctg
     4321 gcgccgcgct tcgcgccgca gcacgtcacc aacatcaagc agctcgttgc cgaaaaattc
     4381 ttcgacgggc tcggcgtcgt ccgcgtgcag gacaacttcg tcgcccagtg gggcgacgcc
     4441 gacaagacgc gtcccaccga aagcgcgctg cgcaagttgc cggatgagaa tgcgttcgcc
     4501 ttcagcaaac gcctgccgtt cgtcaaacta cccgatgtcg acggctatgc gcgcgagacc
     4561 ggctggatcg acggcttccc cgtgggcgtc gagcgcaaac cctcgggtca ggcgtggatg
     4621 acgcactgct atggcgtcgt gggcgttggt cgtgaggatt ggcccgacac cggcagcggc
     4681 gcggaactct acgtcgtgat cggccaggcg ccgcgccagc tcgaccgcaa tatcgtcacc
     4741 gtcggccgcg tattgcgagg catcgaactg ctgtcgtcct tgccgcgcgg cacgggtggt
     4801 gggctgggta tgtatgaaaa gccggagcag cgcgctccca tcaagtccat ccggctcgcc
     4861 gtcgacgttc ctgatgccga gcgaaccgcc atcgaggtgt tgcgcacgga taccccgctg
     4921 ttcgccaggt atgtcgaatc gcgccgcaac cgccgcgata gctggtacat cgtgccggcc
     4981 ggccataccg aggtgtgcag catcaacatt cccgtgcgcg acgtccccgc gcgctagaat
     5041 tcgcgcccta tgggcagaat cttcgaagta cgaaagcaca cgatgttcgc gcgctgggac
     5101 cgcatggcca agcagttcgc gcgcatcgcc aaagacatca acatggccgt caaggccggc
     5161 ggggcggatc cgaattccaa cccggcgttg cggcgtgcgg tgcagaacag ccgcaacctc
     5221 aacatgccca aggacaaggt cgaggcagcc atcaagcgcg ccgcgggcaa ggacgccacc
     5281 gcctacgaac agatcatcta cgagggctac gcgccgcacg gcgtcgcggt catcgtcgaa
     5341 gccgccactg acaatcccac gcgcaccgtg gcgcaggtcc gcgccatctt ctccaagggc
     5401 agcggcaatc tcggcaccac cggcagcgtc gcattccagt tccgcaagat gggtgtgttc
     5461 cgcctgaacc ctgcgggcat cgatcacgat gacctcgagc tctatctcat cgaccatggg
     5521 ctcgaggaga tgggagacag cgtcggcgac aagggcgagc cgcagatcgt ggcgcgatgc
     5581 ctgttcccgg aattcggcca catgcaggaa gcgctcgaga aacgcggcat tcatccgctc
     5641 atcgccaccc acgaatacat ctgcaccacg cccgtggagt tgcccgaggc gcaggccacc
     5701 gaggtcctgg agctcatcga cacgctcgaa cagaatgacg acgtgcagaa cgtctaccac
     5761 tctctagttt agacatggaa gccatcgaag cgctgaacac gcgcgcctcc gcaaagacct
     5821 tcggcgatat cgcccccacg aaagaccatc tggccaccgt gttgcaggcc gcggtccgcg
     5881 cgcccgacca cggccgcctg cggccgtggc gtttcatgct catcgaaggc gaccagcgcc
     5941 ggaaattcgg cgagatgctg gcggcctccg cgctgcgtcg cgttccgggg ctcagtgacg
     6001 gtgatctgca gcgagagcgc ggcaaggctt tgcgcgcacc gctcatcatc gtcgtcgcct
     6061 gccgcatcgt gcccggcacc aagattccgg ccatcgagca gatcctggca gccggcgccg
     6121 ccgcgcagaa catcctgctg gcgttgcaca cgctgggcta tgtcgccgcg tggaaaaccg
     6181 gcgaagcggc gtacgacacg gaagtgaaga agtcgttggg cttcgccgcc gacgatcatc
     6241 tcgtcgggtt catctacacc ggtggcggcg ctggtgcgac gttcgcggcg ggcaaagccg
     6301 ccagcgttca ggatgcgatg ctcgcctttc cgggctgaaa acgcagcagg gcgatgccga
     6361 gaacgaaata accgaagcac acgacgctgg cttcggggcc ttgcggccca ccggtgagcc
     6421 agtccgcgcc caccggtttc aggtctaaca acaatgccgg aatccccaca tcgagtccgg
     6481 tgagcggcag cccgaagccg atggccagca accagttcca gccggcgtgc cagcccatga
     6541 tgccgagcac gctgcgcgtt cgcagcaccc acgcgcaggc aaacaacccg aacaggaacg
     6601 tgccgaccga caccagccag tgagcgcgcg ggctgaagtg caacagcgag aacagcgccg
     6661 agctcacgac taccgcaatg ggcacgttga atttcttcgt cagcaccgac atcagccagc
     6721 cgcggaacaa cagctcttcc acgctcgact gcaacgcgaa cgtgaacagc aggagcacga
     6781 caggcaccag cgacgcaggt tgagaaaatg ccggcgccac cgctgtgagt tcgaggccgc
     6841 cggccatcca gatgatcatg acgatgccga agagccccac cagcccgaac gcgtgaccgc
     6901 gcaggaattc acgaagcttg tgttcaccgc gaataccgat gctcgcgagc gaacggcgct
     6961 ccacgaacct tacccacgcc agcagcaaca gcagcaggaa accgaaggga acgagcgtga
     7021 acgcaaccag cgccgcggga tccagcgggt ttccctcggg gctgatgttt acgaatttcg
     7081 agatcagcat gtcgccgggc aaggcggtgg cgattacgaa taccagcgcc aggaacggcg
     7141 caagcgcgcc ccagggaatc cagccgcggg ccggttctgc cggatagagg tcctgcgaac
     7201 gaaggaatgg catcgggtcc cttgcttatg ggtcgtcgaa tgccagcagt tccgcgcctt
     7261 cgcggacctg gtcgccggga gcacacagca cacgagcgat cctgccggcg gcaggcgcag
     7321 tgatcgtctg ttccatcttc atggcctcga gcaccacgag gggtgcgcct ttggcaacct
     7381 catcgcccgc cttcacgtgt acggccagca cccggcccgg catgatcgcg agcaggccgc
     7441 cgtgtgcgtc ggcgtgttcc gagaccggta gatagggatc gatccactcg aacacccggt
     7501 gttcaccgcc attgaacaga tgtatttcat tgccgaccac gaatgtgcct ggcgcttcgc
     7561 tgccggagaa tgaccccgcg acttcgcgtt cgatctcgcc ctcgcgcagt ttcaggcgcc
     7621 gctccccgcg gacgccgagc gcccaggcgt cacggccatc ccacggtgag cgcgcctgca
     7681 agagagtcgc cttccggcgc tccgccaacc agtgggcctg cgctgccagc tcccacattt
     7741 cgcgtgatgt ctcgccgcgc ggcgcgaaca aatgttcacg ctcgcgttcg atgagcgcgg
     7801 tatccagcgc ggcgtccacg aatgccggcg cactcatgag ccgcccgaga aactcgacgt
     7861 tggtgcgcac tccgacgatc tggtactgcg ccagcgccat ccgcatgcgg gcgatggcct
     7921 cggcccgcgt ctctccatgc acgatgagct tggcgatcat gggatcgtag aacggcgtaa
     7981 tctcatcacc ctgccgtacg ccggtgtcga tgcgcacgtc ggcagtggcc gccggcatcg
     8041 ccacgtgcgc cagcttgccg atcgatggca tgaagtcacg cgacggatct tcggcataca
     8101 cgcgcgcctc gatggcgtga ccggagatcg caagctccga ctgcttgcga ggcaacggct
     8161 cacccgccgc cacgcgcagc tgccactcca cgagatccag ccctgtaatc atctccgtaa
     8221 caggatgctc gacctggagg cgcgtgttca tttccatgaa atagaaggca tcatccgcgg
     8281 caatgaactc cacggtgccc gcaccgcgat agtggaccgc gcgtgccgcg gcgaccgcag
     8341 cttcacccat ctggcgccgg cgttcgggcg tcatgcccgg cgcgggcgct tcttcgatga
     8401 ctttctggtg ccgacgctgg accgagcaat cgcgctcgaa aagatagaca gtgttgccgt
     8461 ggttgtcccc gaacacctgc acttcgatgt gccgcgcggc gctcaggtat ttctccacca
     8521 gcacgcggcc gtctttgaaa ctggaggagg cctcacgctg gcaactggtc agcgcagccg
     8581 cgaaatccgc cgcgcggtcc acgcgccgca tgcccttgcc tccaccacca gacaccgcct
     8641 tgatgagcac ggggaagccg atctgctccg cctgctgcaa tagatagtct ggctcctggc
     8701 gatcgccatg atagcccggc gtgagcggca ctcccgccct cttcatgagc gcttttgcgg
     8761 cggacttcga acccatcgca gcgattgccg cgggggtcgg gcccacaaaa acgacgccct
     8821 cgcgttcgca ggcggtggcg aactccgcgt tctccgaaag aaatccgtag ccggggtgaa
     8881 tggcgtccgc gcccattttc cttgccgccg cgaggatgac ctcgccgcgc agataacttt
     8941 cctggggacg cgagccgccg atgtgcacgg cttcatcgca ggcggcgacg tgcgcggcct
     9001 tcgcgtcggc gtcggaatac accgcgacgg tgcggatacc caatcgcctg gcggtgcgcg
     9061 cgatgcggca ggcaatctcg ccccggttcg cgatcatcag cttggcgaac acgctggagc
     9121 tacttcttcg gcgcccagga aggcgccctt ttctcgagaa acgcggcgat accttcgcgg
     9181 ccttcgggcg acacgcgcgc gtcggcgatg cacttcgcgg tggcggccag caacacatcg
     9241 tccatgggag tttcgaccac gtcggcgagc aggcgtttgg tagcagccaa tgccgccggg
     9301 ctcgcggcca ggaggtgatg cgcgattctc tcgacttcgc gatcgaggcc gtccatcaat
     9361 gtgacttcgt ggaccaggcc cagcgtacgc gcctgcgatg cggtgaattt ttcgcccgtg
     9421 agcatgtagc gcgcggcctg gcgcgcgccc atggcacgga ccaggtgcgg cgaaatggtg
     9481 gccgccacga taccgagctt cacttccgac agcgcaaagt gagcggcttc ggcggcgacc
     9541 acgatatcgc aggcggcggc gaggccgacg ccaccaccaa acgcatcgcc ctgcacgcga
     9601 ccgatgaccg gccgggaaca ggaatgcacg gcatgaaaca tgcgcgccac cttcagtgcg
     9661 tcagcgtggt tgtcggcgtg gctgaaggtc gacatcgaac gcatccagtt caggtcggcg
     9721 ccggcgcaga acgcgggtcc tcgtgaggtc acgaccacca cgcgcgtctc gaccgaagct
     9781 tcgacctccg cgaaagccgc ggcgacttcc gcgaccagag tgtcgttgaa cgcgttgcgg
     9841 acctccgggc gattgagcca caaccaccgt accgcgccgc gctgatcgac ctcgatgtgc
     9901 tggtagttag tcatgcgtca cgcttgctca cattctgaat acgccgaagc gggtttcggg
     9961 aacgggttgg cgcaacgcca cgctcaaacc gagcgcaagg cacatgcgcg tgtcgaccgg
    10021 gtcgatgaca ccatcgtccc agagccgcgc ggtggcgtaa tagggattgc cctgtgcctc
    10081 gtattgctcg cgaatcggcg tcttgaacgc cgcttcttcg tcgctgctcc acgttccacc
    10141 cttgccttcg atggcgtcac gccgcacggt ggccagcacg ttggcggcct gctcgccgcc
    10201 catgacgctg atgcgcgagt tcggccaggt ccacaggaaa cgcggcgcgt aggcgcggcc
    10261 acacatgccg taattcccgg caccgaagct gccgccgacg atcaccgtga acttcggtac
    10321 gcgcgcgcag gcgaccgcgg tgaccatctt cgcgccatgc ctggcgatac cttcgttttc
    10381 atacttcttg ccgaccatga agccggtgat gttctgcagg aacaccagcg ggatgcgccg
    10441 ctggcaacaa agctcgatga agtgtgcgcc tttctgcgcg gactcggaaa acaggatgcc
    10501 gttgttggcg aggatgccca cgggcatgcc gtggatgcg
//
LOCUS       DASQJS010000326         7717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_823807, whole genome shotgun sequence.
ACCESSION   DASQJS010000326 DASQJS010000000
VERSION     DASQJS010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823807"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VEW08_13105"
     CDS             <1..916
                     /locus_tag="VEW08_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYJ41723.1"
                     /translation="AAAKEFITRQVKEGKPFFTWWNGTRMHFRTHVKKEHIGLSGQDE
                     YNDGMVEHDMHVGELLKLIDDLGLANDTIVQYSTDNGPHYNTWPDAGTTPFRSEKNSN
                     WEGAFRVPAFVRWPGHFPAGVTLNGIVSHEDWLPTFAAAAGAPDIKAKLLKGGVSLNG
                     RTYKNHIDGYDLTDYLSGKVKESPRNEFIYVGDDGDVMSIRVGDWKATYLENRAEQLQ
                     VWREPLIKLRLPLLFNLRRDPFERSQHNSNTYHDWMIDRAFVLGPMQMVASEFLLTMK
                     DFPPSQTPGDWSLASLERQIKSMTAPGQ"
     gene            complement(978..1634)
                     /locus_tag="VEW08_13110"
     CDS             complement(978..1634)
                     /locus_tag="VEW08_13110"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HYJ41724.1"
                     /translation="MKRKRLVPLLMAALALPMAARGDTTREFWPELDLWVKLSKPARL
                     LFTLAGTRDREDGEKTDGTFGAYFDYRTSERISVRAGYVYVRGVATEPGETDSIERRL
                     VFDFNYRWRLSERALLVDRTRLDLRDQANEDSYRVRNRLRLEYETKLRDVAINPYASL
                     EAFYDSRHDSVSRYRFEGGVVIPRGQHLEWDVYVGRQRDSQSSTRYTNGIGVTLSFLY
                     "
     gene            complement(1638..2180)
                     /locus_tag="VEW08_13115"
     CDS             complement(1638..2180)
                     /locus_tag="VEW08_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41725.1"
                     /translation="MSAPSKTPIAAIAPPTGRLWAGVAVFAAGWIIGLGLVPFVTSSD
                     LATSVKATLTTLLVVVVPKVFLIGAIAILGKPGFAYLKSVVFGFFKKAAPAAEVGPWR
                     YRLGLVMFLFPFFLGLLLTYIGPVLPGGAEATHRYEKLGDVLQIVSLFVLGGDFWDKL
                     RALFVRDAKAVFPPKPATAA"
     gene            complement(2177..3187)
                     /locus_tag="VEW08_13120"
     CDS             complement(2177..3187)
                     /locus_tag="VEW08_13120"
                     /inference="COORDINATES: protein motif:HMM:NF022616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2955 domain-containing protein"
                     /protein_id="HYJ41726.1"
                     /translation="MDARAHAVMRLAFGVTIAFVAAELLEWTPTFLPPVFAAVLLTNI
                     PVRPPPKVGFSFIILVAGSALIAVLLCAALRGAPIILFGITALVVFRALYALSQGAPI
                     LGPLMLMICITAIPVVSLQSTYVAGQFAYALARAAFVAVMVVWLVYLFWPKVAPPKAA
                     AKPVAMPPALALRSALLGTAIVAPLMLVYLMFGLAQALPAMIATVMIVSSLNLQGGRK
                     QALGLVLANIAGGIASLALILMLVLNPSLATFTLVVLAGSLIIGWRITRGDPMAALVV
                     VAFNACLIVFSSSIGSDSGTFSIWLTRLMHFLIAGAFAVGMMAMLWPRDAQQTTTVET
                     RK"
     gene            complement(3203..4357)
                     /locus_tag="VEW08_13125"
     CDS             complement(3203..4357)
                     /locus_tag="VEW08_13125"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HYJ41727.1"
                     /translation="MDTKATPIEDAPMSEAPPAAPVETKKPRDPVKTITKGVLITLLV
                     LFIYHVASDRVTPYTSQSNIEAMLVQIAPQVSGQVIEVATHDNERVTKGQLLYRIDAE
                     PFEIALRVAEANLALVEQNLKVSTAQVRSTQASLSKQRTELATSKELGGIIFDLTKEK
                     ALSETDSIRARSEIERAGADLARAEADLESAKQKLGPPGDANPQLQQARAALAQARLD
                     FTNTTVLAPADGMITNLRLSRGQYANRGQPVMSFIPGDSVWLTAYLRENQLGKVARGN
                     TVRVAFDLLPGRVFDGKVDSVGWGVTRGGEAPAGTLPDVQSAKGWLREPQRFPVRIAL
                     DAEASSALGNLPLRNGAQANVLILTNPNSWLLNPLGRIWLRIVSWMSYFY"
     gene            complement(4367..5794)
                     /locus_tag="VEW08_13130"
     CDS             complement(4367..5794)
                     /locus_tag="VEW08_13130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HYJ41728.1"
                     /translation="MRGAITAAVLLACGLHSGCTVGPDYVRPEIEAPEKYRFAVVEAN
                     DLANTEWWKQFHDSTLDELISIALSDNFDVRIAAARVDEFYGNLGVTRSGKLPQVGAE
                     AAVGSARTPPNAAADSVRVDAFASWEIDLFGRLRRLTEASRADLLASEEGRRFAVLTL
                     VSTVASTYITLLGVDAQLDIARRTLLSRAEAMRIFEARYKRGASSEFELSQSRSEYAV
                     TKSTIPPLEQAQAQIENALALLLGRNPGPIARTMKIDSLGLPIVPAGLPSEILERRPD
                     IKQAELNLIAANARIGAAKALYYPSISLTGLFGSVSNSFDTLFTGPAELYSYGAAVAG
                     PIFTGGAISGQVSVAEARQQQTLLAYQRAIRTAFRDVENALIASQKSREALLAQKERV
                     DSMREYARLANLRYDNGYSGYLEVLDAERGLFAAELDYTQAKSETFFALVNIYASMGG
                     GWVVDAAALAAQPRVDMAQDPKSFP"
     gene            complement(5809..7149)
                     /locus_tag="VEW08_13135"
     CDS             complement(5809..7149)
                     /locus_tag="VEW08_13135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYJ41729.1"
                     /translation="MNQNWWFTTLLVNEHRHCPPLTRDIKADVVIVGAGFAGVAAAIE
                     FLKTGHKVVLIDKNIVGGSSSGRSAGFLTPDSELELHQLVRRYGVKAAGEIWAAPLSG
                     IERLVAAIQKHDVKCGLIKQDSLFLGLGKGGQKMVDEELHCRQEVGFTDQRTYDQQQL
                     RGILGAQGYTAGIRYGGTYGINPLACLQGFKNLLVDNGMEVYESTEMERLEDHTVYTH
                     GGSITADKIIIAVDKMDESISPLAHEMFHAQTFLSMTEPLTDKELRILFPSGEQMQCW
                     DSKLVYTYFRLTADNRLLLGGGTPITTYLKDAFNSPRIIRKIIKDFYDHFPELSSVSF
                     MQFWPGQIDATRDLLPTIAKPPTQPHIRFIFGAVGIPWAAFTGSFAARNILGAADEDY
                     QKYFNYFSNDRKFSLPSSLGKIIGKPALFALTNSWAKFYQVDKLREAGQLEKEF"
     gene            complement(7192..7554)
                     /locus_tag="VEW08_13140"
     CDS             complement(7192..7554)
                     /locus_tag="VEW08_13140"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HYJ41730.1"
                     /translation="MSAEIASVAGHVLTVKVSGTLTEPELTGMQAAAAKIVGTGGKWR
                     VLVLTENFKGWERGGAWNDFSFSDSDASVERMAIVGDRKWEELALLFTAKGLRPFPIE
                     FFEPERLAAAQDWLAARQ"
BASE COUNT         1496 a         2335 c         2420 g         1466 t
ORIGIN      
        1 ggctgcagcc aaggagttca tcacgcgcca agtgaaggaa ggcaagccgt tcttcacgtg
       61 gtggaacggc acgcgcatgc atttccgcac gcacgtgaag aaagagcaca tcggactctc
      121 cgggcaggat gagtacaacg acggcatggt cgaacatgac atgcacgtgg gcgaactgct
      181 caaactgatc gatgacctcg gactcgccaa cgacaccatc gtgcagtatt caacggacaa
      241 tggcccgcac tacaacactt ggcccgatgc gggcacgacg ccattccgaa gcgagaagaa
      301 ttcaaactgg gaaggtgcgt tccgcgttcc ggccttcgtc cgctggcccg gccatttccc
      361 cgccggcgtc acgttgaatg gcatcgtctc tcacgaagac tggctgccga cgttcgccgc
      421 cgctgcgggc gcgcccgata tcaaggcaaa actgctcaag ggtggcgtgt cgctcaatgg
      481 ccgcacttac aagaaccaca tcgacggcta cgatctgacc gattacctga gcggcaaggt
      541 gaaggaatcg ccgcgcaatg agttcatcta tgtcggcgat gacggagacg tcatgtccat
      601 ccgtgtcggt gactggaagg caacttacct cgagaatcgc gccgagcaat tgcaggtgtg
      661 gcgcgagccc ttgatcaaac ttcggctgcc gttgctgttc aacctgcgtc gtgatccgtt
      721 cgagaggtca cagcacaact cgaacaccta tcacgactgg atgatcgacc gggcttttgt
      781 gctgggtccg atgcaaatgg tggccagcga gttcctgttg accatgaaag attttccacc
      841 cagtcagacg ccgggggatt ggagtcttgc gtcgctcgaa cgccagatca aatcgatgac
      901 cgcgcctggt cagtgagtca atgacagagc gccgccggaa acggcggcgc tttttgaccc
      961 atgcaagagt tgcgggttca atagaggaag ctcagcgtca ctccgatgcc gttggtgtaa
     1021 cgcgtagatg attgtgagtc gcgttgccgg ccgacgtata catcccactc gagatgctgc
     1081 ccgcgcggga tcaccacgcc gccttcgaag cgatatcggc tcaccgagtc gtggcgactg
     1141 tcgtaaaagg cctcgagact tgcgtatggg ttgatcgcga cgtcacgcag cttcgtttcg
     1201 tattccagac gaaggcgatt gcgcacgcga taggagtctt cgttcgcctg gtcgcgcaga
     1261 tctagacgcg ttcgatccac cagcaaggcg cgttccgaca gtcgccagcg gtagttgaaa
     1321 tcgaaaacca ggcgacgttc gatgctgtcg gtctcgccgg gctccgtggc gacgccacgc
     1381 acgtagacgt agcccgcgcg gacggagatg cgctcgctgg tgcgatagtc gaagtacgcg
     1441 ccgaacgtgc cgtcggtctt ctcgccatcc tcgcgatcgc gcgtgccggc gagcgtgaac
     1501 agaaggcggg ccggcttcga aagcttcacc cacaggtcga gttccggcca gaactcacga
     1561 gtggtgtcgc cgcgggcagc cattggcagt gccaacgccg ccatcaacaa aggcacgagg
     1621 cgttttcgtt tcaaggttca ggcggctgta gcgggtttcg gcgggaatac ggctttggca
     1681 tcgcgcacga acagcgcgcg cagcttgtcc caaaagtccc cgccgagcac gaacagactc
     1741 acgatctgca gcacgtcgcc cagcttctca tagcggtgcg tcgcctccgc gccgccgggc
     1801 agaaccgggc cgatatacgt gagcagcagt ccaaggaaga aaggaaacag gaacatcacg
     1861 aggccgagcc gataccgcca agggccgact tctgcggcag gcgcggcctt cttgaagaaa
     1921 ccaaatacca ccgatttcag gtaggcgaag ccgggcttgc ccaggatcgc gatcgccccg
     1981 atcagaaata ctttgggtac gaccaccacc agcaaggtgg tgagcgtcgc cttgactgaa
     2041 gttgccaggt ccgagctggt tacgaacggc accagtccga ggccgatgat ccagcccgcc
     2101 gcgaaaacag caacgcctgc ccagagacgg ccggtggggg gagctatcgc ggcgatgggt
     2161 gttttgcttg gcgcactcac ttcctggtct ccacagtcgt tgtttgttgc gcgtcacgag
     2221 gccacagcat cgccatcatt ccgaccgcaa acgcgccggc gatgaggaag tgcatgagcc
     2281 gcgtcaacca gatggaaaac gttcccgagt cggacccgat gctcgagctg aacacgatca
     2341 ggcaggcgtt gaatgcgacc acgacaaggg ccgccatcgg atcgccgcgc gtgatgcgcc
     2401 agccgatgat gagtgatccc gccagcacca ccagcgtgaa tgtcgcgagc gaggggttga
     2461 gcaccagcat caggatcaac gccaggctcg cgatgccacc ggcgatgttt gccagtacga
     2521 gccccagtgc ttgtttgcgt ccgccttgca ggttgaggct ggacacgatc atgacggtcg
     2581 caatcatcgc gggaagagcc tgggcgaggc caaacatgag atacacgagc atcaagggag
     2641 cgacaatcgc ggtgcccagc agcgcactgc gcaatgccag cgcgggcggc atcgccactg
     2701 gcttggccgc ggccttcggc ggggcgacct tgggccagaa taggtagacc agccagacga
     2761 ccatgactgc gacaaaagcg gctcgcgcca gcgcatacgc gaactggccg gcgacatacg
     2821 tcgactgcag ggagaccacc gggatggcgg tgatgcagat catcagcatc agcgggccga
     2881 ggatgggtgc cccctggctc agcgcgtaca gggcgcggaa caccacgagc gccgtgattc
     2941 cgaacaggat gatgggtgcg ccgcgcaaag cggcacacaa cagcactgca atcaatgcgg
     3001 atccagcgac caggatgatg aaactgaagc cgacctttgg cggcggccgc acgggaatgt
     3061 tggtgagcag cacggccgca aacaccggcg gcagaaacgt gggcgtccac tcaaggagtt
     3121 cggcggcgac gaacgcgatc gtcacgccga aggcgagccg catgactgcg tgagcgcgcg
     3181 cgtccattca gtagcccatt ccttagtaga agtagctcat ccaggacacg atgcgcagcc
     3241 agatgcgtcc gaggggattc agcagccagg aattgggatt ggtgaggatc agcacgttgg
     3301 cctgcgcccc attgcgcagt ggcaggttgc cgagcgcact gctggcttcc gcgtcgagcg
     3361 cgatacgcac tggaaaccgc tgcggctcac gcagccagcc tttagccgac tgcacgtcgg
     3421 gcagggtacc cgcaggtgcc tcgccgccgc gcgtgacgcc ccaacccaca ctgtcgacct
     3481 tgccgtcgaa cacgcggccc ggcaacaggt cgaacgccac gcgcacggta ttgccgcggg
     3541 ccaccttgcc gagctgattc tcccgtagat aggccgtgag ccagacgctg tcgcctggga
     3601 tgaaactcat cacgggttgt ccgcgattgg cgtattgacc cctggacagg cgcaggttgg
     3661 tgatcatgcc gtcagccggc gcgagcacgg tcgtgttggt gaagtcgagt cgcgcctgtg
     3721 cgagcgcggc gcgtgcctgt tgcaactgtg gattggcgtc acccggaggc cccagtttct
     3781 gcttcgcgct ttccaggtcc gcctcggcgc gagccaggtc cgcaccggcg cgctcgattt
     3841 ccgagcgggc cctgatggaa tccgtttccg acagcgcttt ttccttggtc aggtcgaaga
     3901 tgatgccgcc aagttccttg ctcgtcgcga gttcggtgcg ctgcttcgag agcgaggcct
     3961 gcgtcgagcg tacctgggcg gtggaaacct tgaggttctg ctccaccagc gcgaggttgg
     4021 cttcagcgac gcgcagcgcg atctcgaaag gttccgcgtc gatgcgatac agcagttgcc
     4081 ctttcgtcac gcgctcattg tcgtgtgtgg cgacttcgat cacctgcccg gatacctggg
     4141 gagcaatctg caccagcatc gcttcgatgt tcgactgcga ggtgtaaggc gtgacgcggt
     4201 ccgaagccac gtgatagatg aacagcacca gcagcgtaat caggacgccc ttggtgatgg
     4261 ttttcaccgg atcgcggggc ttcttcgtct caaccggtgc agcgggcggt gcctcgctca
     4321 tcggtgcgtc ttctatgggg gtcgccttgg tgtccatgtg aattcttcag ggaaatgatt
     4381 tcggatcttg cgccatgtcg acgcgaggct gggccgcgag ggctgcggca tcgactaccc
     4441 agccgccacc cattgatgca tagatgttca ccaacgcaaa gaaggtttca ctcttggcct
     4501 gtgtgtagtc gagttccgcg gcgaacaggc cgcgctcggc atcgagcact tcgagatacc
     4561 ctgagtatcc gttgtcatag cgcaggttgg cgagccgcgc gtattcgcgc atcgagtcca
     4621 cgcgctcttt ctgtgccaac agcgcttcgc gcgatttctg actggcgatc aacgcattct
     4681 cgacatcacg gaaggccgtg cgaatcgcgc gttgataggc gagcaacgtt tgctgctggc
     4741 gcgcctccgc aacacttacc tggccggaaa tggcgccgcc ggtgaagatc ggtccggcga
     4801 ccgcggcgcc gtagctgtag agctcggcgg gacccgtgaa caaagtgtcg aaggagttgc
     4861 tgacgctgcc aaacagcccg gtcaacgaaa tgctggggta atacagcgcc tttgccgcgc
     4921 caatgcgtgc attcgcggcg atgaggttga gctcggcctg cttgatgtcc ggccggcgtt
     4981 cgagaatctc cgagggcaga cctgccggaa cgatcggcaa tccgagcgaa tcgattttca
     5041 tggtgcgtgc aatggggccg ggattgcgtc cgagcagcaa tgccagcgcg ttttcgatct
     5101 gtgcctgcgc ctgttcgagc ggcgggatcg ttgactttgt gacggcgtat tctgaacgtg
     5161 actgggacag ttcgaattcc gagctggcgc cacgcttgta acgcgcttca aagattctca
     5221 tcgcctccgc gcgactgagc agggtgcgcc tcgcgatgtc cagctgcgca tccacgccga
     5281 gcagggtgat gtacgtcgag gccaccgtcg aaacgagcgt gagcaccgcg aagcgccggc
     5341 cttcttcgct cgccagcaag tccgcacggc ttgcttcggt gagtcggcgc aatcgcccga
     5401 acaggtcgat ttcccaggat gcgaacgcgt cgacgcgcac gctgtccgcg gcggcgttgg
     5461 gtggcgtacg cgcgctgccg acggcggctt cggcgccgac ttgcggcaat tttcctgatc
     5521 gggtaactcc gaggttgcca tagaactcgt cgacacgagc cgcggcgatg cgcacatcga
     5581 agttgtcgga aagtgcaatg ctgatgagct catccagcgt cgagtcgtga aactgcttcc
     5641 accattccgt gttggcaagg tcattggctt ccacgacggc gaagcgatat ttctccggcg
     5701 cttcgatctc gggccggacg tagtcgggtc cgaccgtgca cccggagtga aggccgcagg
     5761 caagcaagac cgctgcagtg atggcgccgc gcatgaggct cgcgaacctc agaattcctt
     5821 ctccagctgg ccggcctcgc gcagtttgtc gacctggtag aacttcgccc agctgttggt
     5881 cagtgcaaac agcgctggct tgccgatgat cttgccgaga ctgctcggca gcgagaactt
     5941 gcgatcgttg gagaaatagt tgaagtactt ttgatagtcc tcgtcggcgg cgccgaggat
     6001 gttgcgcgcc gcgaagcttc ccgtgaacgc cgcccacgga atgcccaccg caccgaaaat
     6061 gaagcggata tgcggctgtg tcgggggttt cgcgatcgtc ggcaacaaat cgcgtgtggc
     6121 gtcgatctgc cccggccaga attgcatgaa cgacacgctg ctcagttccg ggaagtgatc
     6181 gtaaaagtcc ttgatgatct tgcggatgat gcgcgggctg ttgaatgcat ccttgagata
     6241 ggtggtgatc ggcgtgccgc cgccgagcag cagccggttg tcagcggtca accggaagta
     6301 ggtgtacacg agcttcgagt cccagcactg catctgttcc cctgacggga ataggattcg
     6361 cagctccttg tcggtcagcg gctcggtcat gctcaggaac gtctgcgcgt ggaacatttc
     6421 atgcgccagt ggactgatgg actcgtccat cttgtccacc gcaatgatga tcttgtcggc
     6481 cgtgatgctg ccgccatgcg tgtacacggt gtggtcttcg agccgctcca tctccgtgct
     6541 ctcgtagact tccatgccat tgtcgaccag caggttcttg aacccctgca ggcacgccag
     6601 tggattgatg ccgtatgtgc cgccgtaacg aataccggcc gtgtagccct gggcgccgag
     6661 gattcccctg agctgctgct ggtcgtacgt gcgctggtcg gtgaacccca cttcctgtcg
     6721 gcagtgaagc tcctcgtcca ccatcttctg gccgcctttg cccagcccca ggaacagcga
     6781 gtcctgcttg atcagaccgc acttgacgtc gtgcttctgg atggccgcga ccaggcgctc
     6841 gatgcctgag agcggggctg cccagatttc tccggccgcc ttgacgccat agcggcgtac
     6901 gagctggtgc aactcgagct cgctgtcggg cgtgaggaat ccggcgctgc gaccggagga
     6961 gctgccgccg acgatgttct tgtcgatcag caccaccttg tggccggttt tcaggaactc
     7021 gatggctgcc gcgacaccgg caaatccggc gccgacgatg accacatcgg ccttgatgtc
     7081 gcgggtgagc ggcgggcagt gccgatgctc attgacgagc agcgtggtga accaccagtt
     7141 ctgattcacc aagcggaatt tcttagccaa tgtgcacctg tcttgtcgca attattggcg
     7201 ggcggccagc cagtcttggg ccgcagccaa ccgttcgggc tcgaaaaact cgatcggaaa
     7261 tgggcgcaat cccttggccg tgaacagcag cgcaagctct tcccatttgc ggtcgccgac
     7321 aatcgccatg cgctcgacgc tggcatcact gtcggagaac gaaaagtcgt tccatgcacc
     7381 gccgcgttcc caccccttga agttctcggt gagcacgagt acgcgccact tgccgccagt
     7441 gccgacgatc ttcgcagccg ccgcctgcat acccgtgagc tcgggctcgg tcagcgtgcc
     7501 actcactttt acggtcagca cgtgaccggc cacgctggcg atttcggcac tcatggggag
     7561 aaacctcgcg ttgcgcataa ctagttcgcc gcgatgcggc agcaatatcg aaccgtgcgc
     7621 cgccgacggg aatgagacct tggtcccatg atcggaagtg cgccttccgg tatcaaatca
     7681 gctaataaat ccctggggcg cgcaatgaat gttggac
//
LOCUS       DASQJS010000327         8836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_202784, whole genome shotgun sequence.
ACCESSION   DASQJS010000327 DASQJS010000000
VERSION     DASQJS010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202784"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1801
                     /locus_tag="VEW08_13145"
     CDS             <1..1801
                     /locus_tag="VEW08_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HYJ41731.1"
                     /translation="FFQPKPPLTLLVTSPLNVGTKGRVLYYDVGRSASVVMLAQDGGL
                     ALRTNGLPEALMDSPGSLQRFSGEFWLSPLAVIARPNTQNMLIVGFGGGVVVEGVPPS
                     VRRIDVIELEPKVIEANRVTSGLRKRNPLTDPRVNIILNDARGALRLTSRRYDAIVSQ
                     PSHPWTAGASHLYTREFMQLAHDHLTLDGVFVQWMNVIFMDEDLMRSLTATMLSVFGE
                     VRVYRPDPNTVVFLASDQPLNPELQLAQTGYPLRGAPLHYARFGINNAEDLLTALVLD
                     AEGARRLAAGAPLITDDDNRIATSSIYEQRRGMTGESSGRLVAGMDPLQRADSVAYGP
                     LKDLMSFQYIARRTSVFSMLDASLSDRIARMAQILAGDARGEYLRAFYYRMNRQPQRS
                     SELLRLAIDDYPADDSLRLDFLRGAFNELAMDKAPPEIVEVAAKLNPRPAGILAAARH
                     AARNEWRDVAMADAQLAEVPWTDVWYAEALELRVNWRIRITGDKERKRFGDEAIMMID
                     RLAIMSPTLNLYGLRTHAGFSAGRPEIVIESVSNYARLARGMVSAGINTAESVRNDAR
                     ALSGVLDNVATLPGADAARIAEVRAEIKIISGS"
     gene            complement(1806..2843)
                     /locus_tag="VEW08_13150"
     CDS             complement(1806..2843)
                     /locus_tag="VEW08_13150"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HYJ41732.1"
                     /translation="MRWKILALLAGSAACANAAQYPLADAEALMFGDIETITAFGEDT
                     LPDLARRYGLGYEEILRANPGVDTWLPGEGTTIVIPGQRLLPPGPREGIVVNLPEHRL
                     YYFPKPKKGEVPQVITFPVSIGKMDWNTPLGKTRIVDKRKNPTWSPPESVRKEHAERG
                     DPLPAVVKAGPDNPLGAYAMRLGITPGAYLIHGTNNPIAVGMAITHGCIRMYPEDIEA
                     LFPLVPVNTPVWLINEPVKVARVGGQVWLEVHPPVDAEGQRAEVDLEGFYARANAALG
                     ETPAAIHWDFVLTTLKDASGLPQMIGLELDPEMLPAPPEVPSEPVPAQLPAPPAPSTP
                     VGDVPAQPAAG"
     gene            2883..3446
                     /locus_tag="VEW08_13155"
     CDS             2883..3446
                     /locus_tag="VEW08_13155"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HYJ41733.1"
                     /translation="MHKSIHSAAVNDEQLAYLRQSIRTIADYPKPGIQFRDITTLLRD
                     ARAFEITIDAMIDPWRDRQIDKVAGIEARGFILGGAVARGLRCGFVPIRKRGKLPHET
                     VRVAYSLEYGVDEMEMHTDAIRAGENVLLLDDLIATGGTATAAAKLLQSAGAQVIAAC
                     FVIDLPGLGGAARLRAAGNSVHSLLTF"
     gene            3447..4172
                     /locus_tag="VEW08_13160"
     CDS             3447..4172
                     /locus_tag="VEW08_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ41734.1"
                     /translation="MATQPHIFVLIAGAWHGAWCWAKVAPLLVSQGHRVLTPELPATG
                     ADPTDPAGITLDTWAQFVASTVENEPAPVTLVGHSRGGIVISRAAELAPDRLRRLVYL
                     SAYLLPAGGSLAESARADRESLVPPNMIPAEGGVTCNLRAEVIRPALFGQCTDADYEY
                     ALARMTPEPLKPLVTPLKLTEPRFGRVPRAYIECLQDRTVTLAAQRRMQAALPCDPVL
                     TIDSDHSPFLSHAQELAHLLGGL"
     gene            complement(4158..4571)
                     /locus_tag="VEW08_13165"
     CDS             complement(4158..4571)
                     /locus_tag="VEW08_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ41735.1"
                     /translation="MPITQMQHYMVLAKDLEKTRAFYCDVLGLRTGPRPPFKFKGLWI
                     YVGDIAAIHVGERASYDETSRTPANPADPVDHGSGSVDHIAFAATDYDELTASFARHG
                     VKFRSTQVPGSDLRQLFVFDPDGIQIEINIRSKPA"
     gene            complement(4622..5524)
                     /locus_tag="VEW08_13170"
     CDS             complement(4622..5524)
                     /locus_tag="VEW08_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41736.1"
                     /translation="MELLEHWRLAAIAALFFVGVILRRAEWAGPAHGRWLLNFVFYVG
                     LPILIFGALTGATLSREHAVLPALAVCTGLVCWAVAAFLARRLGLPKKSEGAMSLCAM
                     SMNIAMIYPFASLSLGTVAFSQLVMFDMGHAVMAWTLTTVVACKYGGHTDDIPVLLRR
                     ALAAPPLWILFLALGLNVADAPISDDLLHKVLVVGQLLVLLVPLAMGLLVSAAGMRRR
                     EVIASVVLRSALGGLVGFGLALLFGLEGTAAGIAALGASAPVGFTAVVLSAREGLDLE
                     LVASAAAISVFLGSLWLPFAVTMI"
     gene            5569..6228
                     /locus_tag="VEW08_13175"
     CDS             5569..6228
                     /locus_tag="VEW08_13175"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HYJ41737.1"
                     /translation="MIELFTAATPNGWKISIALEEMGLPYTMRALALSKLEQKEEWYL
                     KINPNGRIPAIIDHDNGDFAVFESGAILIYLAEKSGKFLPTEPKARSRVLQWLMFQMS
                     GVGPMMGQANVFYRYAPEKIPYAIERYQREVRRLFEVMERQLGVSEYIAGPDYSIADI
                     ALWSWIAGYGWSGVSVDGLAQMKRWMDLIGARPAAQRGQNIPPPSDPGNVAESGRSIL
                     A"
     gene            6286..6963
                     /locus_tag="VEW08_13180"
     CDS             6286..6963
                     /locus_tag="VEW08_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41738.1"
                     /translation="MNSEPRSSLMHRYLDPASSLGEVVFGLIMTLTFTLAAGIIIDEE
                     GTEGVRELLIALIGCNIAWGVIDGAMYLMGQLFDRGRKRRVGASVRGAPDGAAAMAIV
                     EGELDTWLDDVMTVPERQALYKRVATIVRDKPGAAGFITKADLMGAFTSFCLVVIASA
                     PAAIPFLMIDDSHLALRISNGILLALLFATGFSWAKYTLGKPWMVGFSFLTVGLGLVA
                     LAIALGG"
     gene            complement(6983..7546)
                     /locus_tag="VEW08_13185"
     CDS             complement(6983..7546)
                     /locus_tag="VEW08_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HYJ41739.1"
                     /translation="MTLSATLHAAEPVKPLPVIVGIVTKVSDGDSIEVRLDSGPARVR
                     FSAVDTPEYDQPYGTHSSAALKAMLPVDSKVELEVVTQDQFHRFVATVWLVAGDKRIN
                     INEAMLRDGHAWAYRRYMKDPKFCDIEAEARDARRGLWAQPVSDWVYPPEWRLLRNGE
                     IRTLPAPYAETREKCVAVLGLAGAATY"
     gene            7867..>8836
                     /locus_tag="VEW08_13190"
     CDS             7867..>8836
                     /locus_tag="VEW08_13190"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HYJ41740.1"
                     /translation="MTAASLAHRLQRLGVTIRLVESSSIGTIGVGEATVPAIRRYFQS
                     LGLDAFDVMKATNGTIKLAIEFDGWKHEGHSFMHPFGRFGLEAGPVAFHHLWNRLRRQ
                     GDPGTLDEYAMGTQLARAGRMTLPPDNSRFDFEHFDWAVHFDASRFAAFLRQFAEARG
                     VNRIDARVAEVLRHGESGHIRSVRLESGEILDGELFIDCSGFHRLLIERTLQAGFVDW
                     RHWLMCDRAVALPCAAADPRSIAPYTRSRALDAGWTWRIPLQNRVGNGYVYSSDHISD
                     EQALAALRGQLEGEVLGEPNVVKFRAGHVRQFWIGNCVAIGLASGFL"
BASE COUNT         1615 a         2872 c         2765 g         1584 t
ORIGIN      
        1 cttcttccag ccgaagcctc cgctcacctt gctggtgacc tcaccgctca atgtcggaac
       61 caaggggcgc gtgctctatt acgacgtcgg tcgcagcgcg agtgtcgtga tgctggcgca
      121 ggacggcggg ttggccttgc gcaccaatgg ccttcccgaa gcgttgatgg acagtcccgg
      181 atcgctgcag cgcttcagcg gagagttctg gctgtccccg ctcgccgtga tcgcgaggcc
      241 caacacgcaa aacatgctca tcgtcgggtt tggtggcggc gtggtggtcg aaggtgtgcc
      301 tcccagcgtg cgcaggatcg acgtcatcga gctcgagccg aaggtcatcg aggccaaccg
      361 tgtgaccagc gggctgcgca agcgcaatcc cctcacggat ccgcgcgtga acatcatcct
      421 caacgacgcg cgcggtgcgc tgcggctcac cagccgccgc tacgacgcca tcgtctcgca
      481 gccttcgcat ccatggaccg ccggtgcgtc tcacctgtac acgcgtgagt tcatgcagct
      541 cgcgcatgac cacctcacac tggatggcgt tttcgtccag tggatgaatg tcatcttcat
      601 ggacgaggac ctgatgcgtt cgctgaccgc gacgatgctc agcgtgttcg gcgaggtgcg
      661 cgtctatcga cccgatccga acactgtcgt gttcctggcc agcgatcaac cgctgaatcc
      721 ggagctgcaa ctggcgcaga ccggctaccc gttgcgcggt gcgcccctgc actacgcgcg
      781 gtttggcatc aacaacgccg aggatctgct gacggcactc gtgctcgacg cggaaggcgc
      841 gcgacgcctc gccgccggcg ctccgctgat caccgacgac gacaatcgta tcgcgacctc
      901 gagcatctat gagcagcggc gtggcatgac gggcgagtcc agcgggcggc tggtggctgg
      961 catggatccg ctgcaacgcg ccgattccgt tgcctacgga ccgctcaagg acctgatgtc
     1021 tttccagtac atcgcgcggc gcaccagcgt gttctcgatg ctggatgcca gtctttccga
     1081 tcgcatcgcg cgaatggcgc agattcttgc cggcgatgca cgcggtgaat atctgcgcgc
     1141 cttctactac cgcatgaacc gccagccgca gcgctcgagc gaattgctgc gcctggccat
     1201 cgacgactac ccggccgacg attccctgcg cctcgatttc ctgcgtggcg cgttcaatga
     1261 actggcaatg gacaaggctc ctccggaaat cgtcgaggtc gccgcaaaat tgaatccgcg
     1321 cccggcgggc attctggccg ccgcgcggca tgccgccagg aacgaatggc gtgacgtggc
     1381 gatggccgat gcgcaactcg cggaggttcc ctggaccgac gtctggtatg cggaggcact
     1441 cgagttgcgc gtgaactggc gcattcgcat caccggtgac aaggaacgga agcgcttcgg
     1501 cgacgaggcc atcatgatga tcgaccggct cgcgatcatg agtccgacgc tgaacctcta
     1561 tgggctgcgc acgcacgccg gattctcggc cggccgacct gagatcgtga tcgaatcagt
     1621 gtccaactac gcgcgcctgg cgcggggcat ggtgagcgcc ggcatcaaca cggccgagtc
     1681 ggtgcgcaac gatgccaggg cgttgagtgg agttctcgac aacgtagcga cgcttcccgg
     1741 cgcggacgcg gcgcgcatcg ccgaggtgcg cgccgagatc aagatcattt ccggaagctg
     1801 aagagtcagc ctgccgccgg ctgcgcaggg acatcaccga ccggtgtcga gggcgcagga
     1861 ggcgccggca attgcgcggg caccggctcg gacgggactt caggcggcgc cggcaacatt
     1921 tccggatcga gctcgaggcc gatcatctgc ggcaggcctg atgcatcctt cagggtcgtc
     1981 agcacgaaat cccagtggat agccgccggc gtttcaccca gcgccgcatt ggcgcgcgcg
     2041 tagaagcctt ccaggtcgac ctccgcacgc tgtccctcgg catccacggg cggatgcact
     2101 tcgagccaca cctggccgcc gacccgggcg actttgacgg gctcgttgat gagccacacc
     2161 ggcgtattca cgggcacgag cggaaacaac gcttcgatgt cctcgggata catgcgaatg
     2221 cagccgtggg tgatggccat gcccacggcg atcgggttgt tagtgccatg aatgaggtag
     2281 gcgcccggag taatgccgag acgcatggcg tacgcgccga gcggattgtc aggaccggcc
     2341 ttgaccacgg ccggcaaggg gtcaccgcgt tcggcgtgtt ccttgcgtac cgactcgggc
     2401 ggactccagg tcgggttctt gcgcttgtcg acgatgcgcg tcttcccgag cggcgtattc
     2461 caatccatct tgccgatgct caccggaaag gtgatgacct gggggacctc gcccttcttt
     2521 ggcttcggaa agtagtagag gcgatgctcg ggtagattga ccacgatacc ttcgcgtggg
     2581 cccggcggca ggagtcgctg accgggaatg acgatggtcg tgccctcgcc cggcagccag
     2641 gtatcgacac cgggattggc gcgcaggatt tcctcgtagc ccaggccgta gcgccgtgcg
     2701 agatccggca atgtgtcctc gccgaatgcc gtaatggttt cgatatcgcc gaacatgagc
     2761 gcttcggcgt ccgccagtgg atactgcgcg gcgttcgcgc aagccgcgct gccagccagc
     2821 agcgcgagaa ttttccaacg catcgacaac accttgtaaa gaaccaattt ctggacacag
     2881 tcttgcacaa atccatacac tcggccgccg tgaatgacga acagctcgcc tatctgcgcc
     2941 agtcgatccg caccatcgcg gactacccga aaccgggcat ccagttccgt gacatcacca
     3001 cgctgctgcg cgatgccagg gctttcgaaa tcaccatcga cgcgatgata gatccctggc
     3061 gcgaccgcca gatcgacaag gtcgccggca tcgaagcgcg cggattcatt ctcggcggcg
     3121 cggtcgcgcg cggcctccgc tgtggtttcg tgccgatccg caagcgcggc aagcttccgc
     3181 acgagaccgt gcgggtcgcg tattcgctgg aatacggcgt cgacgaaatg gaaatgcata
     3241 cggacgccat tcgcgccggc gaaaacgtcc tgctgctcga cgacctcatc gccaccggtg
     3301 gcactgcgac cgcggcggcg aagctgctgc agtcggcggg cgcccaggtc atcgccgcct
     3361 gcttcgtcat cgatctgccc ggtctcggcg gcgccgcccg cctgcgagcc gccggcaact
     3421 ccgtgcactc cctgctgacg ttctgattgg ccacgcagcc gcatatcttc gtgctcatcg
     3481 cgggcgcgtg gcatggcgcg tggtgctggg cgaaagtggc gcccctgctc gtatcgcagg
     3541 gccaccgcgt actcacgccc gagctgcccg ccaccggcgc cgaccccacg gatcccgcgg
     3601 gtatcacgct cgatacctgg gcgcaattcg tcgccagcac ggtcgagaat gaacccgcgc
     3661 ccgtgacgct cgtcggccac agccgcggcg gcatcgtgat cagccgcgcc gcggagctcg
     3721 ccccggatcg cctgcgaagg ctggtgtatc tctcagccta tctacttccc gcgggtggca
     3781 gcctggcgga gtccgcgcgc gccgaccgcg agtcgctggt gccgcccaac atgattcccg
     3841 ccgagggcgg tgtcacctgc aacctgcgcg cggaggtgat ccggcctgcg ctgtttggtc
     3901 aatgcacgga cgcagactac gaatacgcgc tcgcgcgcat gacaccggag ccgctgaagc
     3961 cgctggtgac tccgctgaag ctgaccgagc cgaggttcgg ccgcgtgccg cgtgcctaca
     4021 tagaatgcct gcaggaccgt acggtgacac tggccgccca gcggcgcatg caggcggccc
     4081 tgccctgcga cccggttctc accatcgatt cggaccattc gcccttcctc tcgcacgcgc
     4141 aagagctggc gcatttgtta ggcgggcttt gaacggatgt tgatttcgat ctggatgccg
     4201 tccgggtcga acacgaacag ctgccgaagg tcgctgcccg gcacctgagt cgaacggaac
     4261 ttcacgccat gccgcgcgaa gctcgcggtc agctcgtcgt agtcggtggc cgcaaacgcg
     4321 atgtgatcca cgctgccgct gccatgatcc accggatccg cggggttggc cggagtgcgc
     4381 gaagtttcgt cgtaactcgc acgctcgccg acgtgaatgg cggcgatatc acccacgtag
     4441 atccacaggc ctttgaattt gaacggcggc cgcggccccg tgcgcagccc gagcacgtcg
     4501 cagtagaacg cgcgtgtttt ctcgagatcc ttggcgagca ccatgtagtg ctgcatctgt
     4561 gtgataggca tagtggggcc attatatcaa tgagttacaa cctttttctc aggtactttt
     4621 cctagatcat cgtcacggcg aacggtagcc acaacgaccc caggaaaacc gaaatcgctg
     4681 cggcgcttgc gaccagttcg aggtcgagac cttctcgggc cgaaagcact accgccgtga
     4741 atcccacggg agccgatgcg cccaatgccg caattccggc cgccgtacct tcgaggccaa
     4801 acaacagtgc caggccaaat ccgaccaggc cgccgagcgc ggagcgtaac accacgctcg
     4861 caatcacttc gcgtcggcgc atgccggcgg cactcacgag aagtcccatc gcaagcggca
     4921 ctaacaacac cagcagctga cccaccacga gaaccttgtg caggagatcg tcggagatcg
     4981 gcgcgtcggc cacattcagc ccgagcgcga gaaagagaat ccacagcggt ggggccgcca
     5041 gcgcacggcg cagcagaacg ggaatgtcgt cggtatgtcc accgtacttg cacgccacga
     5101 cggtggtgag cgtccacgcc atcacagcat gacccatgtc gaacatgacg agctgcgaaa
     5161 atgcgacggt acccaggctc agcgacgcga acgggtagat catcgcgatg ttcatcgaca
     5221 tggcgcacag cgacatcgcg ccttcactct tcttcggaag gccaagccgg cgagcgagga
     5281 atgccgccac cgcccagcac acgaggccgg tgcagaccgc aagcgcgggc aacaccgcgt
     5341 gctcgcgcga cagcgtcgcg ccggtcaacg caccgaagat caggatcggc aggcccacat
     5401 agaacacgaa gttgagcagc cagcggccgt gcgcaggtcc ggcccactct gcgcgccgca
     5461 ggatcacgcc tacgaaaaag agcgcggcaa tcgccgcgag gcgccagtgt tccagaagtt
     5521 ccacttcgcc aatctatact gcgcccctta cctacgcgag gagcccccat gatcgagctg
     5581 ttcaccgcgg cgacgcccaa tggctggaag atctccatcg cgctcgagga aatgggcctt
     5641 ccgtacacga tgcgagcgct ggccctgtcg aagctcgagc agaaagaaga gtggtatctc
     5701 aagatcaacc ccaacggccg catcccggcg atcatcgacc acgacaacgg tgacttcgcg
     5761 gtgttcgagt ccggcgccat cctcatctat ctcgccgaga agagcggcaa attcctgccc
     5821 accgaaccca aggcgcgctc gcgcgtgctg cagtggctga tgttccagat gagcggagtg
     5881 ggtcccatga tgggccaggc gaacgtcttc taccggtatg cgccggaaaa gattccgtac
     5941 gccatcgagc gctatcagcg cgaggtgcgc cgcttgttcg aagtcatgga gcgccagctc
     6001 ggtgtcagtg aatacatcgc tggaccggac tactcgatcg ccgacatcgc gctgtggagc
     6061 tggatcgcgg gttatggctg gtcgggagtg agcgtcgacg gtctggcaca gatgaagcgc
     6121 tggatggatc tcatcggcgc gcgaccggcc gcgcagaggg gacagaatat tccaccgcct
     6181 tcggatccgg gaaacgtcgc ggaatccggg cgcagtatcc tcgcgtagcc aaaacgctgc
     6241 ggaaggttga tagcgggcct gcggcgcgcc atcgagcatc ggtcgatgaa ctcagaaccc
     6301 cgctcctcgc tcatgcaccg ctatctcgac ccggcttcga gcctgggcga ggtcgtcttc
     6361 ggcctgatca tgacgctcac gttcacgctg gccgccggaa tcatcatcga cgaagaaggc
     6421 accgaaggag tccgtgagct gctcatcgcg ctcatcggat gcaacattgc ctggggcgtg
     6481 atcgacggcg cgatgtatct catgggccag ttgttcgatc gcggccgaaa gcggcgcgtc
     6541 ggcgcatcgg tgcgcggcgc gcccgacgga gccgcggcga tggcaatcgt cgaaggcgag
     6601 ctcgatacct ggttagacga cgtgatgact gttcccgagc gccaggcgtt gtacaaacgc
     6661 gttgcgacga tcgtgcgtga caaaccgggc gcggccggct tcatcacgaa agcggacctc
     6721 atgggagcgt tcacgagctt ctgtctcgtg gtcatcgcca gcgcgccagc cgccatcccg
     6781 ttcctgatga ttgacgactc gcacctcgcg ctgcgcatat cgaacggcat cctgctggca
     6841 ctgctgtttg ccaccggctt ctcgtgggcg aagtacacgc tcggcaagcc ctggatggtc
     6901 ggcttctcat tcctgacggt tggcctgggg ctggttgcgc tggccatcgc gttgggcggt
     6961 tagtagatag gcgtattcgc gctcaatagg tcgcggcgcc agctagcccg agcacggcca
     7021 cgcatttctc gcgcgtctcc gcatacggcg ctggcagcgt gcgaatctcg ccgttcctga
     7081 gcaagcgcca ctcgggtgga tagacccagt cgctcacggg ttgcgcccac aacccgcgcc
     7141 tggcatcacg cgcttcggct tcgatgtcgc agaacttcgg atccttcatg taacggcgat
     7201 acgcccatgc gtgcccgtca cgcaacatgg cttcattgat gttgattcgt ttgtcgccgg
     7261 ccacgagcca gaccgtggcg acgaagcgat gaaactgatc ctgcgtcacc acctcgagct
     7321 cgaccttcga atccacgggc agcatggcct tcagcgccgc tgacgagtgg gtgccatagg
     7381 gctggtcgta ttcaggggtg tcgacggccg aaaagcgcac gcgggccggt ccggaatcga
     7441 gccgcacttc gatggagtcc ccgtcactga ccttggtaac gataccgaca attaccggca
     7501 gaggtttgac cggctctgcc gcatgcaatg tcgcggacaa agtcaatagg caaagcagtc
     7561 tggttcggtt tcgcatgccc ccggtttagc acaacaacaa taaaaaaacc ttcgccgata
     7621 gagggggatc atcggcgaag gttcacaacg ggccccacct gggggctcca tgtctgagtg
     7681 cccaccttgt agccgactcc taacaggtct gcatcagtac ttgtagctag atgcatacgt
     7741 attcgagcgt cccaacccgt ttccaccggt catactgacg tcgtgatttt gaaacctgag
     7801 agtcccaacg cccagtcggc gccagtccgt tccgtggtca ttatcggtgg cggcacagcc
     7861 gggtggatga ctgccgcgtc cctcgctcat cggctgcagc gcctgggggt cacgatcagg
     7921 ctcgtggagt catcgtcaat tggcacgata ggcgttggcg aggccacggt gcccgcgata
     7981 cggcgctact tccagtcact cgggctcgac gcctttgatg tcatgaaggc gaccaacggc
     8041 acgatcaaac tcgccatcga gttcgacggc tggaagcacg agggtcactc gttcatgcat
     8101 cctttcggcc gctttggtct cgaggcgggg ccggtggctt ttcaccacct gtggaatcgc
     8161 ctgcgccggc aaggcgaccc cggaacgctc gacgaatacg ccatgggtac gcagctcgca
     8221 cgcgcgggtc gcatgacgtt gccacctgac aattcacgct tcgacttcga gcatttcgac
     8281 tgggccgtgc atttcgacgc gtcccggttt gcggctttcc tgcgtcagtt cgccgaagcg
     8341 cgcggcgtca atcgcatcga tgcccgcgtt gccgaggtgc tgcgacatgg cgagtccggg
     8401 cacatccgca gcgtccggct ggagtcgggt gaaatcctcg acggtgaact gttcatcgat
     8461 tgtagcggct tccatcgcct gctcatcgag cgcacgctgc aggcgggctt cgtcgactgg
     8521 cggcactggc tgatgtgcga ccgcgcggtt gcgcttccct gcgctgcggc ggatccccgg
     8581 tcaatcgcgc cgtacacacg ttcgcgcgcc ctggacgccg gctggacctg gcgcatcccg
     8641 ctgcagaatc gagtcggcaa tggctacgtc tacagctccg atcacatcag cgacgagcag
     8701 gcgcttgcgg ccctgcgcgg ccagctcgaa ggcgaggtac tcggcgaacc caacgtcgtg
     8761 aagtttcgcg ccgggcacgt acgacagttc tggatcggca attgcgtggc aatcggcctc
     8821 gcgagcggtt tcctgg
//
LOCUS       DASQJS010000328         3656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3971830, whole genome shotgun sequence.
ACCESSION   DASQJS010000328 DASQJS010000000
VERSION     DASQJS010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3971830"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1375
                     /locus_tag="VEW08_13195"
     CDS             152..1375
                     /locus_tag="VEW08_13195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ41741.1"
                     /translation="MTRKQWIVVGALVALITIPVVLKLSRSDTNKVVDLEAATTHALS
                     PTVLASGTLTYESQVTLAPEVTGRVKEILVEEGDQVTRDQLLMRLDPRAPQAAIEQSK
                     AQVRQARLRIERGQVDYDAQVTKVKRYEALKATGMVDVNSLAELVSLRDLAEVDLRTS
                     REQLSQSLAQLSQAEEQLAKTEIRSPLDGKVTAIYVKVGQTAVPSFSGISGSVLLEVA
                     DTTSIDAEINVDETDIANVKVGAEARVVPAAFPDKTLSGTVDQVAIAPRQQAGQNKSY
                     PVRIRLANTEGVVFHPGMSCRAEVLTGTKGDVKLLAVPVQALRYEDNPDKSSKEDKSL
                     ASVFVHAGGRATKRAVTTGTADDSYIAITSGLKEGEQIIVGPAKTLLFLLDGEKVNVN
                     AAAKTAANAEPAAKK"
     gene            1382..2068
                     /locus_tag="VEW08_13200"
     CDS             1382..2068
                     /locus_tag="VEW08_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ41742.1"
                     /translation="MIELRGIGKTYQMGSNELVALADVNLDIARNEYVALIGPSGSGK
                     STMMNLLGCLDIPTTGTYTLDGEAVAGLSEDDLARVRNRKIGFIFQSYYLMPRTTIAD
                     NVAQPLIYRGLAPSVRRERANAALARVGLEARVLHKPNELSGGQRQRVAIARALVGRP
                     NVLLADEPTGNLDSKTAREIMGVFSDLHADGMTIIVVTHDPAIAAYCQRVIRLHDGRI
                     VEDREQAALA"
     gene            2065..3336
                     /locus_tag="VEW08_13205"
     CDS             2065..3336
                     /locus_tag="VEW08_13205"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ41743.1"
                     /translation="MNEGSPIDEGGRLQAFFYSMQECLRSALGSIRSHRMRSFLTMLG
                     VIIGVASVICIVSLLQGLTQSVMNEFQGIGGNTLIIHSETPQKDRMIGKWARIRPADL
                     ELVKLRVAGVSNVTPRTMINVPGGVRNGSNTSAAPVVATTSWFQLSNQAYPRIGRFIT
                     DSDNDSRRRVVVLGDKARTDLKLPENPLGTFIQLGNDWFKVVGVMEARGELFGQNLDN
                     YVVIPFETGRALAGPGREPDMAITVTVDDLDAIADVTSRINALLRRAHKIGPADEDDF
                     KVESSQSLTETIERVSDTITLVISAVVGISLLVGGVGIMNIMLVSVTERTREIGIAKA
                     LGAPRNFILMQFLIEAMLLAVIGGIIGIGFGWLLGVLAAKIIPHFPPPAVPWWAIVGA
                     TGFSALVGMLFGILPARRAANLAPIDALRHE"
     gene            3336..>3656
                     /locus_tag="VEW08_13210"
     CDS             3336..>3656
                     /locus_tag="VEW08_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41744.1"
                     /translation="MSGARKIIWFPLLTAFLLSACEQRAATPTSADARKPLADEAAPA
                     IPDQPVQGTVLGNAFSADQIILKESRIRFRQGNKFFADREVAIDIPALPRVPVIRSGT
                     IWLSE"
BASE COUNT          696 a         1194 c         1147 g          619 t
ORIGIN      
        1 tatcccgacc ttcctcagct ttgcgctcat gatcatcggt gttcgcgtgt ggagccagcg
       61 gtcctggctt ttcagcgcga ccctggtcgt gctcccctgg gttgtcatct acggcctctg
      121 ggcatacttc gccttccggt aaccaccact catgacacgc aaacaatgga tcgtggtggg
      181 cgccctcgtc gcgctcatca cgattccagt cgtactgaaa ctgtcgcgca gcgacactaa
      241 caaagttgtc gatctcgaag ccgcgaccac gcacgcgctg agcccgacgg tgctcgcgtc
      301 gggaacattg acttacgaaa gccaggtgac gctggcgccg gaggtcacgg ggcgggtgaa
      361 ggaaatcctg gtggaagagg gtgaccaggt cacgcgcgat caactcctca tgcggctcga
      421 tccgagggcg ccgcaggctg ccatcgaaca gagcaaggca caggttcgcc aggcgcggct
      481 cagaatcgaa cgcggccagg tggactacga cgcgcaggtc accaaggtga agcgttacga
      541 agcattgaag gcgacgggaa tggtcgacgt gaacagcctg gctgagctcg tatccttgcg
      601 cgatctcgcc gaagtggacc tgcgcaccag tcgcgaacag ctatcccagt ccctggcgca
      661 attgagccag gccgaggagc agctcgccaa gaccgagatc cgctcgcctc tcgacggcaa
      721 ggtgacggct atctacgtga aggtgggcca gactgcggtg cccagcttct ccggcatctc
      781 gggctcggtg ctgctcgaag tcgccgacac caccagcatc gatgccgaga tcaacgtcga
      841 cgaaaccgac atcgccaacg tcaaggtcgg cgccgaagcg cgcgtggtgc ccgcggcctt
      901 ccccgacaag actttgtcgg gcacggtcga tcaggttgcc atcgcgccgc gacagcaggc
      961 ggggcagaac aagagctacc cggtgcgtat ccgcctcgcg aacaccgagg gcgtggtttt
     1021 tcaccccggc atgagctgcc gcgccgaggt gctcacgggc acgaaaggcg acgtcaagtt
     1081 gctcgcggta cccgtgcagg cgctgcggta cgaggacaat ccggacaaat cgtccaagga
     1141 agataagtcg ctcgcaagcg tcttcgtgca cgccggcggg cgcgccacca agcgcgccgt
     1201 caccacgggc accgcggacg acagctacat cgccatcacc tccggcctga aagaaggtga
     1261 gcagatcatc gtgggcccgg ccaagacgct gctgttcctg ctcgacggcg agaaggtgaa
     1321 cgtcaacgcc gccgccaaga ccgccgccaa cgctgaaccg gccgcaaaga agtaactgcg
     1381 gatgatcgag ctgcgcggca tcggcaagac gtatcagatg ggcagcaacg agctcgtggc
     1441 gctcgccgac gtcaatctgg acatcgcgcg caacgaatac gtggcgctga tcggaccttc
     1501 gggatcgggc aagtcgacga tgatgaacct gctcgggtgc ctggatattc cgaccaccgg
     1561 cacgtatacg ctggacggcg aggccgtggc gggcctcagc gaagatgacc tcgcgcgcgt
     1621 acgcaaccgc aagatcggtt tcatcttcca gagctactac ctgatgccgc gcaccaccat
     1681 tgccgacaac gtcgcccagc cgctgatcta tcgcggcctc gcgccgtccg tgcgccgcga
     1741 gcgcgcgaac gcggcactgg cgcgcgtcgg gctcgaagcg cgcgtcctgc acaaacccaa
     1801 tgagctgtcc ggcggccagc ggcaacgcgt ggccatcgca cgcgcgctcg tcggccgtcc
     1861 gaatgtgctg ctggccgatg agcccactgg caacctcgac agcaagactg cgcgcgagat
     1921 catgggcgtc ttctcggacc tgcatgcgga cggcatgacg attatcgttg tcacccacga
     1981 tccggcgatc gctgcgtatt gccagcgcgt gatacgcctg cacgacggcc gcatcgtcga
     2041 agaccgcgag caggcggccc tggcgtgaac gagggctcac ccatcgatga agggggccgg
     2101 ctgcaggcgt tcttctattc catgcaggag tgcctgcgct ccgcgctcgg cagcatccgc
     2161 tctcaccgca tgcgcagctt cctcaccatg ctcggcgtca tcatcggcgt ggcgtcggtc
     2221 atctgcatcg tctcgctgct gcagggcctc acgcaatcgg tgatgaacga atttcagggc
     2281 atcggtggaa acacgttgat catccactcg gagacaccgc agaaagaccg gatgatcggc
     2341 aagtgggcca ggatccggcc tgccgacctc gagctggtga aactccgcgt cgcgggcgtc
     2401 agcaacgtaa cgccaaggac catgatcaat gtaccgggtg gcgtacgcaa cggaagcaac
     2461 acctcggccg caccggtcgt cgcgaccacg agctggtttc aactctccaa ccaggcctat
     2521 ccgcgcatcg gtcgcttcat caccgacagc gacaacgact cgcgccgccg cgtcgtcgtg
     2581 ctgggcgaca aggcacgtac cgatctcaag ctgccggaaa atcccctggg cactttcatc
     2641 cagctcggca acgattggtt caaggtcgtc ggcgtgatgg aggcgcgcgg cgagttgttc
     2701 gggcagaacc tcgacaacta cgtcgtgatc ccgttcgaga cgggcagggc gcttgcgggc
     2761 cccggacgcg agcccgatat ggccatcacg gtcaccgtcg acgacctcga tgcgatcgcc
     2821 gacgtcacga gccgcatcaa cgcgctgctg cgccgcgcgc acaagatagg gcccgcagat
     2881 gaagacgact tcaaggtgga gtcgtcgcag tcgctcaccg agaccataga gcgagtatcg
     2941 gacaccatca cgctggtcat ctccgccgtg gtcggcatct cgctgctggt gggcggcgtc
     3001 gggatcatga acatcatgct ggtgtccgtc actgaacgga cgcgcgagat cggcatcgcc
     3061 aaggcactgg gtgcgccgcg caacttcatc ctcatgcagt tcctgatcga ggccatgttg
     3121 ctcgcggtca tcggcggcat catcggcatt ggtttcggct ggttgttagg cgtgctcgcg
     3181 gcgaagatca tcccgcactt tccaccgccc gcggtgccgt ggtgggcaat cgtcggcgcc
     3241 acgggtttct ccgcgctggt tggtatgttg tttggcatcc tgccggcgcg ccgcgccgcg
     3301 aacctggcgc ccatcgacgc gctacggcac gagtgatgag cggcgcgcgg aagatcatct
     3361 ggtttccgct gctgacggcc ttcctgttga gtgcctgcga gcagcgagcg gcgaccccaa
     3421 cgtcggcgga cgcgcgcaaa ccgttggcgg acgaggcagc ccccgccata cccgaccagc
     3481 cggtccaggg cacggttctc ggcaacgctt tttctgcgga tcagatcatc ctcaaggaaa
     3541 gcagaatcag gtttcgccag ggcaataagt tttttgccga ccgcgaagtc gccatagaca
     3601 ttcccgccct gccgcgcgtg cccgtcattc gcagcgggac gatctggctt tccgaa
//
LOCUS       DASQJS010000329         9750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2402015, whole genome shotgun sequence.
ACCESSION   DASQJS010000329 DASQJS010000000
VERSION     DASQJS010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402015"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VEW08_13215"
     CDS             complement(<1..194)
                     /locus_tag="VEW08_13215"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ41745.1"
                     /translation="MQFEFNEEQLALQESVRRFFTQRYDFETRRSCLAKPTGSSDEIW
                     QAFAEQGFLSIGLPEAHGGIG"
     gene            complement(198..1394)
                     /locus_tag="VEW08_13220"
     CDS             complement(198..1394)
                     /locus_tag="VEW08_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ41746.1"
                     /translation="MDLEYSAAEKLFRDEIRAFIGKNLPKEVAAKVLGHKRLSREDII
                     GWHRILHTRGWSGPTWPKEFGGPGWTAVEQHIFDEECTAAGAPPVIAFGVRMVAPVIM
                     KYGSAAQQQFFLPRILSGEHWWCQGYSEPGAGSDLASLKTTAVRRGDAYVVNGQKTWN
                     TLGHFADWMFCLARTSTEGRSQEGISFLLIDMKTKGITVRPIALIDGEYEVNEVWLDD
                     VEVPVAQRVGEENKGWTYAKYLLTHERVNIARLGESKRELRSLKAIAARVSNDGRPLI
                     EDVRFRDAIAALEIELRALEYTVLGVLSAEAAGRDPGAISSALKISGSEIQQTLSELK
                     MRALGPRALPHVPEALDAGWQGEPSGAEWWAALSGRYFNMRKTSIYSGSNEIQRNIIA
                     HRLLGL"
     gene            1504..3633
                     /locus_tag="VEW08_13225"
     CDS             1504..3633
                     /locus_tag="VEW08_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HYJ41747.1"
                     /translation="MHHVDYARDGAAAVITISNPPVNSFSHAVRSALLDALDRAAADD
                     GVRVVVLAGAHGRFSGGADIREFGTPASLAAPQLRDLIAVVEASAKPVIAAIDGICLG
                     GGFEIALGCHYRVARAAARVGLPEVKLGLLPGAGGTQRLPRLVGVETALNMILSGEQI
                     PARDLATSALFDRVVDGEPVRAAVEFANELEKQGGLVPRRVSELMVVDPNLEALCDFA
                     MMTVKAKAPLAVAPQRCIECVKAIARPFAEGLAIERRLFVELVQSPESKGLRHSFFAE
                     RAAGKVADISESTPLRPLAKAAVIGAGTMGGGITICFLNAGIPTWLLETDQPALDRGI
                     ARIAGIYDAQVKKGKLEPSERDKRLGLLMPVLKYDVIGAADIVIEAVFERMEVKLAVF
                     AELDRVMKPGAILATNTSTLDVNVIARSTKRPADVLGTHFFSPANVMRLLEIVRGTAT
                     GRDALATALALGKILKKVSVVSGVCDGFIGNRMLEHYGKQANYLLEEGASPRQVDAAM
                     EAFGFAMGPFKVGDLAGNDIGWNIRKRRRAEKPGYKFSTLPDKLCELGRFGQKTGAGW
                     YEYPDGRKPLPSKVVDELIVAHRKELGIRPRKIDDAEIIDRLVYALANEGARILDEGI
                     AARSSDIDVCYLTGYGFPAMRGGPMFHADFVGLPLVQRRMREFARNGHGDPEFWKPAP
                     LIERLVATGGSFATGAPASAPVKGVAA"
     gene            3630..4808
                     /locus_tag="VEW08_13230"
     CDS             3630..4808
                     /locus_tag="VEW08_13230"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HYJ41748.1"
                     /translation="MSTDAVIVSTARTPLAKSFRGAFNLSHGAHLGGVVVRAALERAH
                     IEPGEVEDVIMGCAYPEGSTGGNVARISAYRAGCPATVAGTTVSRFCSSGLQTIALAA
                     QRIMAGEDGIYVAGGVESISTVQPNRNQHMARDSWTATHKPDLYLPMLDTAEIVSKRY
                     GIPRERMDEYGVRSQIRACAADAAGHLRDEIIATPATMAVVGEDGRFATRDVVVEHDE
                     GLRADTTYEGVSKIRAAQPGGAIAAGNASQFSDGAGACVVMSAREAARRNLTPLGVFR
                     GFAVAGCEPDEMGIGPVFAVPKLLKRAGLTVQDVGLWELNEAFAVQVLYCRDRLGIDD
                     DRLNVDGGAIAIGHPFGMSGQRLTGHALIAGKRLGARHVVVTMCVGGGMGAAGLFEVV
                     "
     gene            4835..6184
                     /locus_tag="VEW08_13235"
     CDS             4835..6184
                     /locus_tag="VEW08_13235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ41749.1"
                     /translation="MSTEQQKANAKKALLLLFAVNLLNFFDRQLPGVLGEPIRKEFGL
                     DDAQLGLIGTAFTLMYAAVGLPIGRLADTWVRTRLIAAGLTVWSALTAVSGMVHTYSG
                     LVAARLGVGIGEATCSPACQSLIGDLYPPEKRSRALGAFMLGLPFGLCLSYLTGAWLG
                     AEYGWRTCFMLAALPGFIVAVLVLRLPEPVRGGADGFVAPGASQPPKHATSWEGYKRV
                     MRIRTLWWIVISGALHNFNVYAINAFQTPFLQRYHALDLRNAALVSALVVGLVGAIGL
                     LGGGWASDRLSRRRADGRLLLASVTMMASVPCVFLALAQPPGSIIAFMIPMALGTTLS
                     FVYYATVYAAIQDVVEPRLRGSAVAIYFFAMYVMGASMGTYLTGKLSVFLAKRAMAEA
                     GASAMTEVFRAVGLHDAMYVIPTLAFCCALVLFAASRTVGADMVVNRAGRSAFVDKV"
     gene            6188..7219
                     /locus_tag="VEW08_13240"
     CDS             6188..7219
                     /locus_tag="VEW08_13240"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HYJ41750.1"
                     /translation="MPTMKAAVIHAFKEPLRIEEVPVPEVVPGRILVKVVASGVCHTD
                     LHAADGDWVFKPKPPFIPGHEAAGHVSAVGAGVKHVKEGDRVGVPWLHTTCGHCEHCI
                     SSWETLCDRQQITGYTVNGGYAEYVLADPAYVGRLPANIDFSAIAPVLCAGLTVYKGL
                     KQLERKPGDWVAISGVGGLGHLAVQYARAMGFHVAAVDIDDRKLELARKLGAELVVNA
                     MSADPVKELQRALRGAHGVLVTAVSPKAFEQALGMLHKRGTMVIVGLPPGEFALPIFD
                     VVGNAKTIRGSTVGTRMDLQEAIAFAAEGKVQTVYTEDKLENINNIFDRMRKGAIEGR
                     VVMKLSGGS"
     gene            complement(7266..7793)
                     /locus_tag="VEW08_13245"
     CDS             complement(7266..7793)
                     /locus_tag="VEW08_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41751.1"
                     /translation="MKTPRRNILAILDNAVRWTGIPARVADDMSGAPDRKHRPLRWIP
                     IWMIAFSCTLFILSLAWPPALDMVAITVSFMGSIAAIAPGIHICGPLGKSSLEDDERE
                     AALRKDSFLFCFGLLTLLNCVGQLLLMSLSHLQNWQTAQSVFVAASAFMLNLTLFGCL
                     PTLYASWNMPQLPKE"
     gene            complement(7790..8035)
                     /locus_tag="VEW08_13250"
     CDS             complement(7790..8035)
                     /locus_tag="VEW08_13250"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYJ41752.1"
                     /translation="MSLYNRISVFRAEQGISRRQLADAVGVNHQTIGYLERGDYKPSL
                     ELALKIARHFGVAVEMLFSFERFEPIAMTFKRAGEDE"
     gene            complement(8116..8463)
                     /locus_tag="VEW08_13255"
     CDS             complement(8116..8463)
                     /locus_tag="VEW08_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41753.1"
                     /translation="MNLNEDRVDHHRAMIIGTIAAVHFLLSVALFFLMMASGMSRFEG
                     RGPSDLTHHLLNSAFEIWTFPLVTGFRLLEIANTGIWGWLVFLGNSALWGWVGWRAIR
                     FWRSRHGVAPENL"
     gene            complement(8466..9713)
                     /locus_tag="VEW08_13260"
     CDS             complement(8466..9713)
                     /locus_tag="VEW08_13260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ41754.1"
                     /translation="MPSHDRYLSRRQLLQTFAVAGGMGLSGCATPELLGSAEADRIGP
                     NDRARFLAPLRARTDRIFDITVCLRPFRAAGPRLDTERIRDTLVVHNYGHGGSGWSLS
                     WGSSTIAVDKALAASKALAQSSDRIAVVGCGVLGLTSALLAQAAGKQVTIYAREMIQQ
                     TTSARASGSFTPDSRIALADAVSPEFPALWEAMARTSLKAFRSHLGLPGDPVRWIDRY
                     VVSDHVVSNQATPVERPPAEGELQFAAYFNRLQDLFPRGETLRPTDTPFRADSVRRRS
                     VLMFNIASYAQILMTQFHLAGGRIVQREFHDPQELAGLPEKVVIDCTGYGARALWRDE
                     SVVPVRGQIAYLIPQAEFDYALAYRDVNVIPRSDGIVVQAVAGGDMRGYYDESLVPDR
                     QESDAAVQTLTALYSKFRRTERD"
BASE COUNT         1722 a         3032 c         3213 g         1783 t
ORIGIN      
        1 ccgatgccgc cgtgcgcctc gggcaggccg atggagagaa aaccctgttc ggcgaatgcc
       61 tgccagatct cgtcgctcga gcctgtgggt ttcgcgagac agctgcggcg cgtctcgaag
      121 tcgtagcgct gcgtgaagaa gcgccgcacg gattcctgca gggcaagttg ttcttcgttg
      181 aattcgaact gcatttgtca gagccccagc aggcggtgcg cgatgatgtt gcgctggatt
      241 tcgttcgagc cactgtagat ggacgtcttg cgcatgttga agtagcggcc ggagagggcg
      301 gcccaccact cggcgccgct cggctcgccc tgccagccgg cgtcgagtgc ctcgggcacg
      361 tgcggcaaag cgcgcggtcc gagcgcgcgc atcttgagct cggacagcgt ctgctggatc
      421 tcggagccgg agatcttgag cgccgaggag atggcgcctg gatcgcggcc cgcggcctcg
      481 gccgagagca cgcccagtac ggtgtactcg agcgcgcgca gctcgatctc gagcgcggcg
      541 atggcgtccc ggaagcgcac gtcttcgatg agcgggcgac catcattcga cacgcgggcg
      601 gcgatggcct tgagcgagcg cagctcgcgc ttggactcgc cgagccgcgc aatgtttacg
      661 cgttcgtgcg tcagcaggta cttcgcatac gtccagccct tgttctcctc gccgacacgc
      721 tgcgcaacgg gcacttcgac gtcgtcgagc cacacctcgt tcacctcgta ctctccatct
      781 atcagcgcga tgggacgcac ggtgatgcct ttcgttttca tgtcgatgag caggaatgag
      841 atgccctcct gcgagcgtcc ctcggtggac gtccgcgcca ggcagaacat ccagtccgcg
      901 aagtggccca gcgtgttcca cgtcttctgg ccgttcacga cgtaggcgtc gccgcgccgc
      961 accgcggtgg tcttgagcga ggcgaggtcg gagcccgcgc cgggctcgct gtagccctgg
     1021 caccaccagt gctcgcccga gaggatgcgc ggcaggaaga actgctgctg cgcggcggag
     1081 ccgtatttca tgatgacggg cgcgaccatg cgcacgccga atgcaatgac gggtggcgcg
     1141 cccgcggccg tgcactcctc gtcgaagatg tgctgctcga ctgcggtcca gcccgggccg
     1201 ccgaattcct tcggccacgt ggggcccgac cagccgcgcg tgtgcaggat gcggtgccag
     1261 ccgatgatgt cctcgcgcga gagacgcttg tgtcccaaca ccttggcggc gacttccttc
     1321 gggaggttct tgccgatgaa ggcgcggatt tcgtcgcgga acaatttttc ggcggcggag
     1381 tattcgaggt ccatcgcggt atccgtattc cgctgtgcgg aatgttgttt tgcaaaactt
     1441 gaaagcagac taagccctaa gtaatgcttc atcaagttgc accggcaatt ccaggagcat
     1501 ttcgtgcacc acgtcgatta cgcgcgcgac ggcgcggcag cggtcataac aatctcgaat
     1561 ccgccagtga acagcttctc gcacgcggtc cgttccgcat tgctcgatgc gctcgaccgc
     1621 gctgctgcgg acgatggtgt gcgggtcgtc gtgctcgcgg gtgcccatgg aaggttctcg
     1681 ggcggcgcgg acatccgcga gttcggtacg cctgcgtcac tcgcggcgcc acaactgcgc
     1741 gacctcatcg cggttgtgga ggcgagcgcg aagcccgtga tcgcggcgat cgacggcatt
     1801 tgtctcggcg gcggtttcga gatcgcgctg ggctgtcact atcgagtggc gcgcgccgct
     1861 gcgcgcgtcg gtttgcccga ggtgaagctc ggcttgctgc cgggcgccgg cggcactcag
     1921 cggctgccgc ggctcgtcgg cgtggagacc gctctcaaca tgatcctgag cggcgaacag
     1981 attccggcgc gcgacctcgc gacgtcggcg ctgttcgatc gcgtggtcga cggcgagccc
     2041 gtgcgtgcgg cggtggagtt cgccaatgaa ttggagaagc agggtggttt ggtgcccagg
     2101 cgtgtctcgg agctgatggt tgtcgaccca aacctcgaag cgctgtgcga tttcgcgatg
     2161 atgaccgtca aggccaaagc cccgctggct gtcgcgccgc agcgttgcat cgaatgcgtg
     2221 aaggccatcg ccaggccgtt tgcggagggg ctggcgattg agcgccggct attcgtcgag
     2281 ctcgttcagt cgccggagtc gaagggcctg cgccattcgt tcttcgcaga gcgcgcagca
     2341 ggcaaagtcg ccgatatttc cgaatccacg ccgctgcgcc cgctcgcgaa ggccgccgtc
     2401 atcggcgctg gcaccatggg cggcgggatc accatctgct ttctgaatgc gggcattccc
     2461 acctggctgc tcgagaccga ccagcccgcg ctcgaccgcg gcattgcgcg tatcgcgggc
     2521 atctacgatg cgcaagtgaa aaagggcaag ctcgagccgt ccgagcgcga caaacgcctg
     2581 gggctgctga tgcccgtgct gaaatacgac gtcatcggcg ccgcggacat cgtcatcgag
     2641 gcggtgttcg agcgcatgga agtaaagctc gcggtgtttg ccgagctcga tcgcgtcatg
     2701 aagcccggtg ccattcttgc aaccaacact tcgacgctcg atgtcaacgt catcgcgcgc
     2761 tcgacgaagc ggccggccga cgtgctgggc acgcactttt tctcgcctgc caacgtcatg
     2821 cggctgctgg aaatcgtgcg cggcacggcg acgggccgtg acgccctggc cacggcgctg
     2881 gcgctcggca agatcctcaa gaaggtctcc gtcgtttccg gcgtctgcga cggtttcatc
     2941 ggcaaccgca tgctcgagca ttacggcaag caggcgaact atctactcga ggagggcgcg
     3001 agcccgcgcc aggtggacgc agcgatggag gcgttcggct ttgcgatggg tccgttcaag
     3061 gtcggagacc tggccggcaa tgacatcggc tggaacatcc gcaagcgccg ccgcgcggag
     3121 aagccgggct acaagttctc gacgctgccc gacaagctct gcgagctggg ccgcttcggg
     3181 caaaagaccg gcgcgggctg gtacgaatac cccgacggcc gcaagccgct gccatcgaaa
     3241 gtggtggacg agctcatcgt ggcgcaccgc aaggagttgg gcatcaggcc gcgcaagatt
     3301 gacgacgcgg agatcatcga caggctcgtg tacgcgctgg ccaatgaagg cgcgcgcatc
     3361 ctcgatgaag gcatcgcggc gcgttcgtca gacatagatg tgtgttatct caccggctat
     3421 ggctttcccg ccatgcgcgg cgggccgatg ttccatgcgg acttcgtggg tcttccgctc
     3481 gtgcagcggc gcatgcggga attcgcgcgc aacggccacg gcgatccgga gttctggaaa
     3541 cccgcgccgc tcatcgagcg tctcgttgcg accggtggtt cgttcgcgac cggggctccc
     3601 gcgtccgccc cggtgaaagg tgtcgcggca tgagcactga cgcggtcatc gtctccaccg
     3661 cgcgcacgcc gctcgcgaag tccttccgag gcgccttcaa cctgagccat ggcgcacatc
     3721 tcggcggcgt ggtcgtgcgg gccgctctcg agcgcgcgca catcgaaccc ggcgaggtgg
     3781 aggacgtcat catgggctgc gcctatcccg agggctccac gggtggcaac gtcgcgcgca
     3841 tcagcgccta ccgcgccggt tgcccggcga cggtggccgg caccacggtc agccgcttct
     3901 gcagctcggg cctgcagacc atcgcgctcg ccgcacagcg catcatggcg ggcgaagatg
     3961 gcatctacgt cgcgggcggc gtggagagca tttccaccgt gcagccgaac cgcaaccagc
     4021 acatggcgcg tgactcgtgg acggcgacgc acaagccgga tctctatcta cccatgctcg
     4081 acacggccga gatcgtgagc aagcgctacg gcatcccgcg cgagcgcatg gacgagtacg
     4141 gcgtgcgcag ccagatccgc gcctgcgccg ccgatgccgc gggccacctc cgcgacgaaa
     4201 tcatcgcgac gccggccacc atggcggtcg tcggtgagga tggccgcttc gcgacacgcg
     4261 acgtagtggt ggagcacgac gagggcctgc gcgccgacac cacttatgag ggcgtgtcga
     4321 agatccgtgc ggcgcagccg ggcggggcca tcgctgcggg caatgccagc cagttctccg
     4381 atggcgcggg agcctgcgta gtaatgagtg cgcgcgaggc cgcgcgccgc aacctcacgc
     4441 cgctcggcgt gttccgcggc ttcgccgtcg cgggctgcga gcccgacgaa atgggcatcg
     4501 gcccggtgtt cgccgtgccc aaactactga agcgcgcagg gctcacggtg caggacgtcg
     4561 ggctctggga gctcaacgaa gctttcgccg tgcaggtgct ctattgtcgc gaccggctcg
     4621 gcatcgacga cgatcgcctc aacgtcgatg gtggcgccat tgccatcggt catccattcg
     4681 gcatgagcgg gcagcgcctc acgggtcatg cgctcatcgc gggcaaacgc ctcggcgcgc
     4741 gccacgtggt ggtgacgatg tgcgtgggcg gcggaatggg cgctgcgggg ttgttcgaag
     4801 tggtctaggg cgggcgagtc atgggcgagg ggtcgtgagc acagaacaac aaaaagcgaa
     4861 cgcgaagaag gcgctgctgc tgctcttcgc tgtcaacctg ctcaacttct tcgatcgcca
     4921 gctacccggc gtgctcggcg agccgattcg caaggagttc ggcctggacg acgcgcagct
     4981 cgggttgata ggcacggcgt tcacgctcat gtacgcggcc gtgggcctgc cgatcggtcg
     5041 gctcgcggat acctgggtgc gcacgcggct catcgccgcc ggcctcacgg tgtggagcgc
     5101 gctcacggcc gtctcgggca tggtgcacac ctattccggg ctcgtagccg cgcggctcgg
     5161 cgtgggcatc ggcgaggcga cgtgctcacc cgcgtgccag tcgctgatcg gtgacctcta
     5221 tccacccgag aagcgctcgc gcgcgctcgg cgcgttcatg ctcggccttc catttggcct
     5281 gtgcctttcc tatctcaccg gcgcgtggct gggcgcggag tacggctggc gcacgtgctt
     5341 catgctcgca gcgttgccgg gattcatcgt cgcggtgctg gttctcagac tgcccgagcc
     5401 cgtgcgcggc ggagctgatg gatttgtcgc gccgggcgcg tcgcaaccgc cgaaacatgc
     5461 gacgagctgg gaaggctaca agcgcgtgat gcgaatccgg acgctgtggt ggatcgtgat
     5521 ctcgggcgcg ctccacaact tcaatgtgta cgcgatcaac gcgttccaga cgccgttcct
     5581 gcagcgctac cacgcgctcg atctgcgcaa tgccgcgttg gtgtcggcgc tggtcgttgg
     5641 attggtcggc gccattggct tgctcggcgg cggctgggcc tcggaccgcc tgagccggcg
     5701 ccgcgccgat gggcgcctgc tgctcgcctc ggtcacgatg atggcgtcgg tgccctgtgt
     5761 gttcctcgcg ctggcgcagc cacccgggtc gatcatcgcg ttcatgattc cgatggcgct
     5821 cggcaccacg ctgagcttcg tctactacgc caccgtgtac gcggccatcc aggacgtcgt
     5881 cgagccgcgg ttgcgcggca gcgccgtggc tatctacttc ttcgcgatgt acgtgatggg
     5941 cgcgagcatg ggcacgtacc tgacgggcaa gctgtccgtg ttccttgcaa agcgggcgat
     6001 ggcggaggcg ggcgcgtccg cgatgaccga agtcttccgt gcagttggac tgcacgacgc
     6061 aatgtacgtg atccccacgc tggcgttctg ctgcgcgctg gtgctgttcg cggcatcgcg
     6121 caccgtgggc gctgacatgg tggtgaaccg ggccgggcgg tcggcattcg tagataaggt
     6181 gtaatgcatg ccaacgatga aagccgccgt catccacgcg ttcaaggagc ccttgagaat
     6241 cgaggaggtg ccggtgcccg aagtcgtgcc ggggcgcatc ctcgtcaaag tcgtggcgtc
     6301 cggtgtgtgc catacagatc tgcatgcggc cgacggcgac tgggtgttca aaccgaagcc
     6361 gcctttcatt cccggccacg aggcagccgg ccatgtcagc gcggttggcg ccggcgtgaa
     6421 gcatgtgaaa gagggagacc gcgtcggcgt accgtggctg cacaccacgt gcggccactg
     6481 cgagcactgc atcagtagct gggagacgct gtgcgaccgt cagcaaataa cggggtacac
     6541 cgtcaacggt ggatatgccg aatacgtgct tgccgatccc gcgtatgtgg gccgcctgcc
     6601 cgcgaatatc gatttttcag ccattgcccc agtgctctgc gcgggactga ctgtctacaa
     6661 aggtctgaag cagcttgaac gcaagcctgg tgattgggtt gccatctcgg gcgtcggcgg
     6721 cctgggtcac ctggcggtgc agtacgcccg cgcgatgggt tttcacgtcg ccgctgtcga
     6781 catcgatgac aggaaactcg agcttgcgag gaagctcggc gctgagctcg tcgtcaatgc
     6841 gatgagcgcg gaccccgtca aggagttgca gcgcgcactg cgtggggcgc atggcgtgct
     6901 ggtcacggcg gtgtcaccca aggccttcga gcaggcattg ggaatgctgc acaagcgcgg
     6961 caccatggtg atcgttggcc ttccaccggg cgagttcgca ttgccgatct tcgatgtcgt
     7021 gggcaatgcg aagaccattc gtggatcgac cgtcggtacg cgaatggatt tgcaggaagc
     7081 gattgcgttt gccgctgaag gcaaggtgca aacggtctac accgaagaca agctagagaa
     7141 catcaacaac atcttcgatc gcatgaggaa gggcgccatc gaaggtcgcg tcgtgatgaa
     7201 gctgtccggc ggaagctgac gccgttcctg gggtcgagag gcgatctctt cgattgcggg
     7261 tgcggttatt ctttcggcaa ttgcggcatg ttccagctcg catagagtgt tggcaaacac
     7321 ccgaacaagg tcagattcag catgaaggcg gatgctgcga caaagacact ttgcgcagtt
     7381 tgccaattct gcaaatgaga caggctcatg agcaagagtt ggccgacgca gttgagtaag
     7441 gtgagcagcc caaagcaaaa caggaaggag tcctttctca gcgccgcctc gcgctcatca
     7501 tcttcgagcg aagactttcc caacggcccg cagatatgga tacccggcgc tattgccgcg
     7561 atgctgccca tgaatgacac ggtgatggcg accatatcca gcgctggcgg ccaggccaat
     7621 gaaagaatga acagggtgca cgaaaaagcg atcatccaaa tcgggatcca gcgaagcggg
     7681 cggtgcttgc gatcgggtgc accggacatg tcatcggcga cccgcgcggg tattcccgtc
     7741 catcgcacag cgttatccag aatcgcaaga atgtttcggc gaggcgtttt cattcatctt
     7801 ctcctgctcg cttgaacgtc atcgcgatgg gttcgaagcg ctcgaacgag aaaagcattt
     7861 cgacagccac tccaaagtgg cgcgcgatct tcagggcgag ttccaggctc ggcttgtaat
     7921 cgccgcgttc gagataacca atcgtctgat ggttgactcc gaccgcgtca gccaattgcc
     7981 gccgggagat gccttgctcg gcgcgaaaaa cggaaattct gttgtataga ctcatgcaaa
     8041 tgttgagaaa ctcaactaat cgttgtgatt acacaacgac tctgtccgaa aagtcaacac
     8101 tcgcccggcg tgaattcaga gattctctgg cgcaacaccg tgccgggatc gccagaaacg
     8161 aatcgctctc cagcccaccc agccccaaag agcgctattg ccgagaaaca cgagccaacc
     8221 ccagatcccc gtgtttgcga tttccagcag ccggaatccg gtcaccaacg gaaaagtcca
     8281 tatctcgaat gcactgttca acaggtggtg agtgagatcc gacgggcccc ggccttcgaa
     8341 ccgcgacatg ccactcgcca tcatcaggaa aaaaagtgcg acggaaagca gaaaatgaac
     8401 cgctgcgatc gtgccgatga tcattgcgcg atgatgatca acgcggtctt cgttcagatt
     8461 catagtcagt cgcgctctgt tcggcggaac ttgctgtaaa gcgcggtcag cgtctgcacc
     8521 gctgcatcgg attcctgccg atccggtacc agcgattcgt cgtagtaacc gcgcatgtct
     8581 ccacccgcga ctgcctgcac cacgatgcca tcgctgcgcg ggatgacatt cacatcgcga
     8641 tacgccagtg cgtagtcgaa ctccgcctgc ggaatcagat atgcaatctg accgcgcacc
     8701 ggcacgacag actcgtcgcg ccataacgca cgcgcgccat agccggtgca gtcgatgacg
     8761 actttctcgg gaaggccggc cagttcctgc gggtcgtgga actcgcgttg cacgatgcgg
     8821 ccaccggcca gatggaactg agtcatcaga atttgcgcgt agctcgcaat gttgaacatg
     8881 agcacgctcc gccgccggac actgtcggca cgaaacggcg tgtcggttgg gcgcagggtt
     8941 tcgccgcgag ggaacaggtc ctgcaagcgg ttgaagtatg ccgcgaactg cagctcaccc
     9001 tccgcgggag gacgttcaac cggagttgcc tggttcgaga caacatggtc cgagacgaca
     9061 tagcggtcga tccagcgtac cggatcgccg ggcaggccca ggtgactgcg aaaagccttg
     9121 agcgaggtgc gtgccatcgc ttcccagagc gccgggaact ccggacttac ggcatcggcc
     9181 agtgcgatgc gcgaatccgg agtgaatgat ccggaagcgc gcgccgacgt agtctgctgg
     9241 atcatctcgc gcgcgtagat agtcacctgt ttgcccgcgg cctgcgcaag cagcgccgag
     9301 gtcagtccaa gcaccccgca cccgacgacc gcgatgcgat cggaagattg cgcgagcgct
     9361 ttgctggcgg ccaatgcctt gtctacagca atcgtgctgg aaccccagga cagcgaccat
     9421 ccgctgccgc catggccata gttgtgtacc accagcgtgt cgcggatgcg ctccgtatcg
     9481 aggcgcggac cggcagcgcg aaagggtcgc aggcacacgg tgatgtcgaa gattcgatcg
     9541 gtacgcgccc gcagcggtgc caggaatcgg gcgcgatcgt tcggcccaat gcggtccgcc
     9601 tcagcactac cgagcagttc gggtgttgcg catccgctca ggcccatgcc gcccgctacg
     9661 gcgaaagtct ggagcagctg gcgcctgctg aggtatctat cgtgcgatgg catgttcgtg
     9721 tcctccctgc aaaacctgcg gcagtctgcc
//
LOCUS       DASQJS010000330         3479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1143109, whole genome shotgun sequence.
ACCESSION   DASQJS010000330 DASQJS010000000
VERSION     DASQJS010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143109"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VEW08_13265"
     CDS             <1..243
                     /locus_tag="VEW08_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ41755.1"
                     /translation="YARENPVDLVITDVNMPRMDGITLVSELRQLPAYRLTPVLLLTT
                     ESSMDKKMEGKKAGATGWIVKPFNPAQLLATLSRVL"
     gene            251..730
                     /locus_tag="VEW08_13270"
     CDS             251..730
                     /locus_tag="VEW08_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41756.1"
                     /translation="MSDLGNTPVSALLELAASQLEVAAHTTDSQVETLANSIAALVTL
                     SVELQAASGAGTAPSGLARRIVEQAQAALVAMQFHDQLVQRMAHVRDALTELNEALSG
                     GPAAEVDWATVLAGIRFRYSMEDERMQFDRIIGSTACPDVTAHDAAHDSLRGSVELF"
     gene            781..1425
                     /gene="cheD"
                     /locus_tag="VEW08_13275"
     CDS             781..1425
                     /gene="cheD"
                     /locus_tag="VEW08_13275"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF010013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HYJ41757.1"
                     /translation="MRTAPDHSTPPPALPQFSHVSRYWDNEYKCFAARLLPGEYYVTN
                     AHEVIATVLGSCVSACIRDTRIGVGGMNHFMLPLDASQGTSAWGAAASAATRYGNVAM
                     ERLINDILKLGGRRENLEIKLTGGGRVLAAMTTDIGARNIEFVRQYVKDEGFNVVGED
                     LGDIYPRKVVYFPDTGKIRVKRFSAAKDESLAERERNYLNELDSKPIGGDVELF"
     gene            1443..2333
                     /locus_tag="VEW08_13280"
     CDS             1443..2333
                     /locus_tag="VEW08_13280"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HYJ41758.1"
                     /translation="MQTGTHATPGNIVRPEARVRDFTAMLEVEIAGDHIDLPSYPEVA
                     LRVRRVLADEDVSIDQVVRVVSAEPSLVVRLLQLGNSAALNPNGRRLTDLRAAITRIG
                     FNMARSATIAFAMSQLRRAEAYRGLEKPFGELWRNSTQVAAVSFVVAKRVAKVNAELA
                     LLAGLLHTVGKLYLLTRAARYPDLLNDAVAYPALVQVWHARIARAILVNWEMAPPVID
                     AVQGFEAANREREGEIDLLDVLWVGRSLAALPRPAPAMPQELLESHPARRLGLDAAAC
                     AEILVESGTEIDSLTSALAD"
     gene            2473..3381
                     /locus_tag="VEW08_13285"
     CDS             2473..3381
                     /locus_tag="VEW08_13285"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HYJ41759.1"
                     /translation="MSLELSTSFPKTRIGDRPAPLAGDARALVDSLAAELASEKIDLP
                     SFPDVAMRVRKALTNEDVGIEDAARIVSAEPALSARLMQLANSATLNPGGKRLNNLLS
                     AMTRIGLNMACSVTIAFAMSQLRRAETYRGIELRLGKLWHEAAHLAAVSRVIARRFTS
                     LNADTALLAGLLQCIGKLYLLTRAARYPSLLEDDAVLDRIGSEYHLRVAQAILRNWDM
                     DAEIIKAIGACENFGREHAGAIDLTDVLTVSAALIALGPAQQSEQLPFPGMPAARRLG
                     LDAEACAAALAESRDEIDALRQALNV"
BASE COUNT          623 a         1118 c         1164 g          574 t
ORIGIN      
        1 tacgcccgtg aaaacccggt ggacctggtg atcaccgacg tgaacatgcc gcgcatggac
       61 ggcatcacgc tggtgagcga gctgcgtcag ctgcccgcct accggctgac gccggtactg
      121 ctgctgacca ccgagtcgag catggacaag aagatggaag gcaagaaggc cggtgccacc
      181 ggctggatcg tcaagccctt caatccggcg cagctgctgg caactctctc gcgggtgctt
      241 tgacccgacg atgagcgatc tcggcaacac acccgtctcc gcattgctcg aactggccgc
      301 aagccagctc gaagtcgctg cgcacacgac cgattcgcag gtggaaacac tggcgaattc
      361 gatcgccgcc ctggtgacgc tgagtgtgga gctccaggcg gcgagtggcg ctggcaccgc
      421 gccgagcggg ctcgcgcgca ggatcgtcga gcaggcgcag gccgcgctgg tggcgatgca
      481 gtttcacgac cagctcgtgc agcgcatggc gcacgtgcgc gatgcgctga ccgaactcaa
      541 cgaagcgctg agtggcggac ccgcggccga ggtcgactgg gcaacggtgc tcgcgggaat
      601 ccgtttccgc tacagcatgg aagacgagcg tatgcagttc gaccggatca tcgggtccac
      661 ggcctgtccc gacgtgacgg cgcacgacgc cgcgcacgac agcctgcgcg gcagcgtcga
      721 gctgttctga tgccaccccg catcagccca catccaaagc aaccgacaca cgcgcgtcgc
      781 gtgcgtacgg cgcctgacca cagcacgccg ccaccggcgc tgccgcagtt ctcgcatgtg
      841 agccggtact gggacaacga atacaagtgt ttcgcggcgc gcctgctgcc cggcgagtac
      901 tacgtgacca atgcgcacga ggtgatcgcc accgtgctcg gttcctgcgt ctccgcctgc
      961 atccgcgaca cgcgcatcgg ggtcggtggg atgaatcact tcatgctgcc cctcgacgct
     1021 tcgcagggta cgagcgcctg gggcgcggcg gcgagcgccg ccactcgata cggtaacgtc
     1081 gccatggagc ggctgatcaa cgacatcctc aagctcggcg gccgtcgtga gaatctcgag
     1141 atcaagctga cgggcggtgg acgcgtgctt gcggccatga ccaccgacat cggcgcgcgc
     1201 aacatcgagt tcgtgcgcca gtacgtgaag gacgaaggtt tcaatgtggt cggtgaagac
     1261 ctgggcgaca tctatccccg caaggtcgtc tactttcccg acaccggcaa gattcgcgtc
     1321 aagcgcttct cggcggcgaa ggacgagtcg ctggcggagc gcgagcgcaa ctatctcaac
     1381 gagctggaca gcaaaccgat tggaggagac gtggagctgt tctgacagcc cgcgaaacac
     1441 ccatgcaaac cggcacccac gcaactccag gcaacatcgt ccggccggaa gcgcgggtgc
     1501 gcgacttcac ggccatgctc gaagtggaga tcgccggcga tcatatcgac ctgccgagct
     1561 accccgaagt ggcattgcgt gttcgccgcg tgctcgccga tgaagacgtg tccatcgacc
     1621 aggtggtgcg cgtggtcagc gccgagccat cgctggtggt gcggctgctg cagctcggga
     1681 actcggccgc gctcaatccc aacggccgcc ggttgacgga tctccgcgcc gcgatcacgc
     1741 gcatcggctt caacatggcg cgcagcgcca ccatcgcgtt cgccatgtcg cagctgcggc
     1801 gcgcggaagc gtacaggggc ctcgaaaaac ctttcggcga gctctggcgc aacagcaccc
     1861 aggttgcggc ggtgagtttc gtggtggcga aaagggtggc gaaggtgaac gccgaactgg
     1921 cgttgctcgc cggcctgctg cacacggtcg gcaaactcta tctgctgacg cgtgcggcgc
     1981 gttatccgga cctgctcaac gacgccgtgg cttatcccgc gctggtccag gtctggcatg
     2041 cgcgcatcgc cagggcgatt ctcgtgaact gggaaatggc gccgcccgtg atcgacgcgg
     2101 tgcagggatt cgaagcggcc aatcgggagc gtgaaggcga aatcgacctg ctcgatgtcc
     2161 tgtgggtggg gaggagcctg gcggcgctgc cgcgaccggc gccggcgatg ccgcaggagt
     2221 tgctcgagtc gcatcccgcg cgccgccttg ggctcgatgc cgcggcgtgc gccgagatcc
     2281 tggtcgagtc cgggacagag atcgattcgc ttaccagtgc tctagccgac tagaggcggc
     2341 aaggccttgc cgccaatccg gtccgtttga catatcgggc tggtatcgcg aaatccagtt
     2401 ccggattccc gccggaaccg ctcaatagtc cgcacggcag accgataagt aatcaggtct
     2461 gagagaggac acatgagtct ggagttgagt acaagtttcc cgaagacgcg gatcggcgat
     2521 cgtccggcgc ccctggccgg tgatgctcgc gcgttggtcg attccctggc ggcggagctg
     2581 gcgagcgaga agatcgactt gccgagcttt cccgacgtgg ccatgcgcgt gcgcaaggcg
     2641 ctgacgaacg aagatgtggg catcgaagat gcggcgcgga tcgtcagcgc cgagccggcg
     2701 ctctccgcgc gtctcatgca gctggcgaat tcggccacgc tcaaccccgg tggcaagcgc
     2761 ctgaacaacc tgctctcggc gatgacgcgc atcggcctca acatggcctg cagcgtgacc
     2821 atcgcttttg cgatgtcaca gctgcgccgc gcggaaacgt acaggggcat cgagctcagg
     2881 ctcggcaagc tctggcacga ggctgcgcac ctggcggccg tcagccgcgt gattgcgcgg
     2941 cggttcacct cgctgaacgc ggacacggcg ttgctggccg gcctgctgca gtgtatcggc
     3001 aagctctatc tactgacgcg cgccgcgcgt tatccgtccc ttctcgagga cgatgcggtt
     3061 ctcgatcgga tcgggtccga atatcacctg cgcgtggcgc aggccatcct gcgcaactgg
     3121 gacatggacg cggagatcat caaggcgatc ggcgcctgcg aaaatttcgg gcgcgagcat
     3181 gccggagcca tcgatctcac cgacgtgctg acagtcagtg ccgcgctgat cgcgctgggt
     3241 ccggcacagc agtcggagca gttgcccttt ccgggcatgc ccgccgcgcg ccggctgggg
     3301 ctggatgccg aggcgtgcgc ggcggcgttg gccgagtccc gcgacgagat cgacgcattg
     3361 cgtcaggcgc tcaacgtatg agccaggcag aaaccaccag cacgatcgag tcgcgcgcga
     3421 ttccgaacac cgtggtgttc ggcttcgtca aggcgctggc cctggagttg tcacagggc
//
LOCUS       DASQJS010000331         3009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2088505, whole genome shotgun sequence.
ACCESSION   DASQJS010000331 DASQJS010000000
VERSION     DASQJS010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088505"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VEW08_13290"
     CDS             <1..210
                     /locus_tag="VEW08_13290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HYJ41760.1"
                     /translation="EAPVALTTGPLPAGHADVLLNLGNTVPDRFEKFARVAEFLDSRP
                     EVRTAGRERFKAYRSKSIEPQTHNV"
     gene            344..2554
                     /locus_tag="VEW08_13295"
     CDS             344..2554
                     /locus_tag="VEW08_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41761.1"
                     /translation="MKMLPGLVVACGALAVAQARPLVLQESARIANPDPGNFPYFAAD
                     VAIDGDDAIATLERYFDAPEGGDPNDDTHDVAVHLFHKTTGGWAPVRQLVLHHHHPFA
                     SFNSGLAMRNGIAALALNPLYVFERRDGDWVSAPVTGVDPAMPGDSITIDGTRILFGG
                     TSGPWQGTLYEKNSATATWGPTSIMVGDYRGGDDEFVGGPVDISGGRAVVLSPYNEEE
                     FPVASPGVTVFRDWGPPSRFLRQSDIRSSPSAPLEYEVAIRGDEIFIAGNNRSGTRVY
                     RPAPSDAWEEFDKLQPLDSYMGGGVTTVIEKNHLFLMQRNWNAEREAYVINVFRKTAA
                     APYEHVATLVAGDGASLGNFGISSRRVIASCGGEACVFDLPSSFTQPQPLQHTFAGAT
                     PAGWSMSAGSAFAIARRGISLVLRQSDTSSPAIHTAVLDASNWSNQSVQADIRPLSFA
                     GSGWHGLATRYRDTGNYYYVTVSGVRVSLNRAAGGSFSTIASAPASIALNRPYRLRLE
                     SIGTRHRVYVNGAPLLDVDDTTLPSGRAALLTHQSSAEFDNVVVSPTPATILYAHDFT
                     SSGGPTDLWSFTGTGLWNNVASGSNRVFAQSSVAGAARAVIGVPTDDLSVDVRARPTT
                     FAGTGGGDRWFGVMARYVDDANYYYLTLRSNNTVSLRKLTNGAITVLGTYSLPVTVGA
                     WYQLRLEAVGARIRGYVNGFLVAEATDSTHTRGAAGPITNRAAVDFDDYRAIQP"
     gene            2665..>3009
                     /locus_tag="VEW08_13300"
     CDS             2665..>3009
                     /locus_tag="VEW08_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYJ41762.1"
                     /translation="MDKAYSPGEIEARIYERWESSGAFAPQGTGPAYCIMLPPPNVTG
                     TLHMGHAFQHTLMDTLTRWHRMRGDAALWQPGTDHAGIATQLVVERQMMERQEPGRHK
                     IGRDRFIEKVWAW"
BASE COUNT          528 a         1041 c          927 g          513 t
ORIGIN      
        1 gaagcgccag tcgcgctgac cacgggcccg ctgccggccg gccacgccga cgtgctcctc
       61 aacctcggca acaccgtgcc ggaccgcttc gagaaattcg cgcgcgtggc cgagttcctc
      121 gattcgcgac ccgaggttcg caccgccggc cgcgagcgtt tcaaggcgta tcgcagcaag
      181 tccatcgagc cgcagactca taacgtctga tctgtaggaa gcgccctccc ccaatcgggg
      241 caaatttcct ccgccagtaa tgcccgcagt cgcgcatcca ccgcgccgca acctctgttt
      301 tgatgcgtgc gaggccagga aggccccggt ctaactacgc cccatgaaaa tgttgcctgg
      361 tctggtggtt gcctgtggcg ctctcgcagt ggcgcaggcg cgcccgctgg tgctccagga
      421 atcggcgcgc atcgcgaacc ccgacccggg caacttcccc tatttcgccg ccgacgtcgc
      481 catcgatggt gacgacgcga tcgcgacgct cgagcgctac ttcgacgcac cggagggcgg
      541 tgatccgaac gacgacacgc acgatgtggc cgtccacctc ttccataaaa ccaccggcgg
      601 atgggcgccc gtccggcagc tcgtactcca tcaccaccac ccgttcgcca gcttcaactc
      661 cggactcgcc atgcgcaacg gcatcgcggc gctggcgctg aaccccctgt acgtgttcga
      721 gcgacgcgat ggggactggg tctccgcccc ggtcaccggg gtcgatccgg ccatgcccgg
      781 tgattccatc accatcgacg gaacgcgcat cctgttcggc ggcaccagcg gtccgtggca
      841 gggaacgctc tacgagaaga actccgccac ggcaacctgg ggccccacct cgatcatggt
      901 gggcgactat cgcggcgggg acgacgagtt cgtcggtggc ccggtcgaca tttccggcgg
      961 tcgggccgtg gtgctcagcc cgtacaacga agaagagttt ccggtggcat cgccgggtgt
     1021 cacggtgttt cgcgactggg gtccgccaag ccgcttcctg cgccagtccg acatccgcag
     1081 ctccccgtcc gcgccgctcg aatacgaggt cgcgatccgc ggcgacgaga tcttcatcgc
     1141 cgggaacaac cggtcaggca cgcgtgtgta ccgcccggcg ccgagcgatg cctgggaaga
     1201 gttcgacaag cttcagccgc tcgacagcta catgggcggc ggtgtcacca cggtcatcga
     1261 gaagaatcat ctcttcctga tgcagcggaa ttggaacgcc gagcgcgagg cgtacgtcat
     1321 caatgtgttt cgcaaaaccg ccgcggcacc ctacgaacac gtggccacgc tggtcgcggg
     1381 cgacggcgca tcgctgggca acttcggcat cagcagccgc cgggtgatcg cgagctgcgg
     1441 gggcgaggcc tgtgttttcg atctgccgtc gagtttcacc cagccgcaac cactccagca
     1501 cacgttcgcg ggagcgacgc ccgcgggatg gtcgatgagc gccggcagcg cgttcgcgat
     1561 tgcccggcgt ggcataagcc ttgtgctgcg tcagtccgac acgtcgagtc ccgcgattca
     1621 caccgcggtc ctcgatgcct ccaactggtc caatcagtca gtccaggcgg acattcgccc
     1681 gctttcgttc gccggcagcg gctggcacgg tctcgcgacg cggtaccgcg ataccggcaa
     1741 ctactactac gtgacagtga gcggcgtccg cgtctcgttg aaccgtgccg cgggcggaag
     1801 cttttcgacc atcgcatcag ctccggcctc catcgcactg aatcgcccat atcggctcag
     1861 gctcgaatcc atcggcaccc gtcaccgtgt ctacgtgaac ggtgcaccac tattggacgt
     1921 cgacgacacg acactcccga gtggacgcgc cgcgttgctc acgcatcaat cgtcagcgga
     1981 attcgacaac gtggttgtga gccccacacc cgcgacgatt ctctatgccc atgacttcac
     2041 gagttccggc ggacccaccg atctgtggtc gttcacgggc acaggtctgt ggaacaacgt
     2101 cgcgagcggc tcgaaccgcg tgttcgcgca gagttccgtg gcgggcgcgg cacgcgccgt
     2161 gatcggcgtc cccaccgacg acctgtcggt cgacgtccgt gcgcgtccca cgacgttcgc
     2221 gggaacaggt ggtggcgacc gctggttcgg cgtcatggca cggtatgtcg acgatgcgaa
     2281 ctactactac ctgacgttgc gaagtaacaa tacggtatcg ctgcgcaagc tcaccaacgg
     2341 cgccatcacc gtgctcggaa cctattcgct gcccgtcacg gtgggtgcct ggtaccagct
     2401 gcgactggag gccgtgggcg cccggatacg cggctacgtc aacggcttcc tggtggctga
     2461 agccaccgat tccacgcata cgcgcggagc ggcgggcccc atcaccaatc gcgccgccgt
     2521 ggatttcgac gactaccgcg ccatccagcc ctgaaaaagg ggacagatct atttatcgct
     2581 gctagccgtt ccgacgctgc caacggcagc tggcgcgata aatagatctg tccccttttt
     2641 cagatccgta taatccgcgc cctcatggac aaggcatatt ctccgggcga aatcgaagcc
     2701 cgcatctacg aacgttggga atcctcgggc gctttcgcgc cgcagggcac tggccccgcc
     2761 tactgcatca tgctgccgcc gccgaacgtc accggtacgc tgcacatggg gcacgcgttc
     2821 cagcacacgc tcatggacac cctcacccgc tggcaccgca tgcgcggcga cgcggcgctg
     2881 tggcagccgg gcacggacca cgccggcatc gccacgcagc tggtggtcga gcgccagatg
     2941 atggagcgcc aggagcccgg ccggcacaag atcggccgcg acaggttcat cgagaaggtg
     3001 tgggcgtgg
//
LOCUS       DASQJS010000332         9504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1459201, whole genome shotgun sequence.
ACCESSION   DASQJS010000332 DASQJS010000000
VERSION     DASQJS010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459201"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VEW08_13305"
     CDS             complement(<1..1092)
                     /locus_tag="VEW08_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ41763.1"
                     /translation="MSKFFRIALVCGALVACAPAMAAKKEVDKLSSTVVRDLHFGDAL
                     FYYFQGQDFDAITRLQAYRQWGRMPHHDAETDLLLGGLYLSLGLHNEAGERFETLLTQ
                     SVPEGVRNRAWFYLGKIWYARGYLDRSERAIKEVQGRLTPDLEAERMHLLSNILMRQQ
                     RFDEAIELLNNWKGRQDQSDWLAYAQYNLGVALVRKGLVTEADPFLTAVGTLESGRRE
                     LRSLRDRANLALGYAYLQNNQPAEAKPVLERVRLDGPFSNKALLGVGWADAAEGQFQQ
                     ALTPWMELRERNLLDSAVQEAYLAIPYAFAKLNANAQAAENYELAIRSFDTETVNLDA
                     SIAEIDDGTMLDKLLQRDANNRVGWFWQLK"
     gene            complement(1101..1739)
                     /locus_tag="VEW08_13310"
     CDS             complement(1101..1739)
                     /locus_tag="VEW08_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41764.1"
                     /translation="MPQLKTFRFAWMTLIILALPALAQDPQAVPPAEAPAADAAAADP
                     AAPAPDAGAEVSATGEPPADTRALDETVQDLKKDVVDLNKELFVLEEELLFPANTQVA
                     VFVSMDIGEFFALDSLTLKIDNKEVANYLYTPREAEALLKGGVHRVYLGNLKVGEHQL
                     VAFFAGKGPNNLDYKRGANLKFEKGVGAKYLELKITDRQRKAQPEFAIKDWE"
     gene            complement(1757..2278)
                     /locus_tag="VEW08_13315"
     CDS             complement(1757..2278)
                     /locus_tag="VEW08_13315"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYJ41765.1"
                     /translation="MHARFAAFALACALGLSLCLGGGAAIAAEKHVLLGQPAPDLVAR
                     DLTGQNVRLSEHRGEVVVVSFWSGACNTCRAQLEALDRIARTYGSAGLTVIGVNLDDN
                     TPRAEKFARALSVNFQLLVATAKTTGRDFSVDRLPMLLFVDRAGVLRVAHREFRSRDE
                     ALYVRELRTLLDE"
     gene            complement(2551..4179)
                     /locus_tag="VEW08_13320"
     CDS             complement(2551..4179)
                     /locus_tag="VEW08_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41766.1"
                     /translation="MIIRTKKSPRLLGLDEPIRHESHKAPVTRRDFIAQGMKTGPAII
                     AAPAALAMLIGSDNARAMSPDLEALLGPAACAIQAGAGKIPFIAFDLAGGANLTGSEM
                     LCGQSGNPLNFLSTQGYATLGLPGNMTPGSANAASPTNNFVNTEFGAAWHSDGAILRG
                     MQASTLAATRANVNGFPIAARSENDTGNNPHNPMYGINRVGADGQLLTLIGSQNTDSG
                     GNSMAPAAMMNPAARPTKVDRASDVTGLVDTGELGTLFSNQDDAIAVLESMTRLSHAK
                     VNQSPTKLAAQEAALHKLVRCGYLKSTYLADRFRTPAALNPNLDPHIVAQASQPGGIT
                     PIFTAAEYNADGEFRKAAAIMKMVINGYAGAGTVTMGGYDYHGQGRNTGETRNFRAGR
                     CIGACLEYAARMGKPLMIYVFSDGSLSANGMVNNTVEGRGKLDWASDNQSTAAPFVLV
                     YKPGQGRPQLRSATRMQIGSLNASGNVVTSSSPAANAVNLLVETVILNYMALHGEEGN
                     FPTLFPSNGLGSVALRDSLTMIAPLVNGTVSNPV"
     gene            complement(4192..6717)
                     /locus_tag="VEW08_13325"
     CDS             complement(4192..6717)
                     /locus_tag="VEW08_13325"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HYJ41767.1"
                     /translation="MNAMQSKILHWFSAATAAAILAGCSSGGAPTVVNPVTTAPPVLD
                     YTGPASANADVQAFRINLWENIKGNNRCGSCHGAGGQTPMFARNDDVNLAYQAANGVV
                     NLTQPDQSRMVVKVAGGHNCWLQSPAACGDTLTVWIRNWAGASATGGTQIQLQAPTIK
                     EVGGSKSFPASSALFASSALYALVRDPARANCGRCHSSTSAAQQQPFFAEGGAVPSPA
                     SIETAYAAIKSKINLANTADSRLVVRLGDESHNCWASGGTCADNAATMLQAINAFVDP
                     LPVNEIPAGMLISKGLTLYDGTVAAGGNRYEAATIAKFEFKTGMGNIAYDTSGHEPAL
                     NLTLSGDVAWVGGWGINVKMGGKAQGTAASSKKLADLIKSTGEFTIEAWANNANVAQE
                     DAFIVSYSAGANARNATLAQNEYTYHGYTRSDKTSANGNPVLITRAADMDAQAALQHV
                     VMTYSPVEGRRIYVNGVYTGDLDAQGGGSLADWDDTFALVLGNETSTNRQWEGVLRLV
                     AIHNRALSPSQIQQNFDAGVGEKYFMLFNVSHLVTVPQAYVMIEASQLDSYGLQFSKP
                     TFISLDPNASVANLQISGVRVGVNGAILHTGQAYEPLDQTIGGANYTAGAGQLMTPVG
                     TVVSIENGPIDDLYFLQFDHIGANDNPLPPPGAPSAPTPVDLPAESDVGLRTFDELNA
                     SLSLITGVSPNNAAVVSTYTLVKQALPSIEKLGAFGPAQQTGLAQLAIQYCSQMVDTP
                     ALRTAFYGGSLNPAASATSTFGSVSSPNTANRDLVITALMTKAKNTGLEWESTVDGMI
                     PAEIDDLIDKLVAGPTGGAANGTGTVMKAACGAVLGSGTTLIQ"
     gene            complement(6728..7903)
                     /locus_tag="VEW08_13330"
     CDS             complement(6728..7903)
                     /locus_tag="VEW08_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41768.1"
                     /translation="MNTPQVSKVKTVGLAVLLASVAIAASANDRDQAKRIYDRIAGVP
                     PSEATLDTMEGMVAGGNAIGAANIATQAPSFYNVTLKNMAVPWTNRDQTVFAPLNDYV
                     ATFIGMVRDDVAFNTALSADLTYTVNGSTPAASASNNTHYENAETNNVNLFTALTPTT
                     QSSIQGIPPAATAGFITSRAASEAFFVDGTNRAMFRFTMMNHMCRDMEQVHDTSLPPD
                     RIRQDVSRSPGGDSRIFLNNCIGCHSGMDPFAQAFAYYDFDNGSEEAPGTMRLVYNST
                     AVHSKYFNNNTNFAPGFITPDDAWENRWRVGQNSLLGWGATPGRGNGAKSMGEELANS
                     DAFAQCQVEKVFRTVCFRAPGNSADRAQVATMTANFQSTGYRLKQVFAETAVYCMGD"
     gene            8233..>9504
                     /locus_tag="VEW08_13335"
     CDS             8233..>9504
                     /locus_tag="VEW08_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41769.1"
                     /translation="MNRPYENANRITLGRLGLGLVLGAAGLLAACTQGPGGGGVGLAS
                     GQDPDPATVDFPIFYIRHQIPEDQDDVTRLRPFVEDDDYSATLWKRDRASPGAPEAEI
                     TARLRVDPLGSPEDRYDIKDLAVSPDGLRVAFAMRGPLDDFDEDEPPTWNIWEYNIAT
                     DTLHRVITSDIIEEEGQDVSPSYLPDGRILFSSTRQRQAKAILLDEGKAQFEAATEAR
                     NESAFVVHVMNADGTGIHQISFNPSSDLYPQVLQNGRVLFTRWDRAPGRDGLHLYTSN
                     PDGTDMQLHYGAVSHLTGTVVAGVATPIEFMRAREMSDGRLMVLTRQRTNVDFGGNLT
                     IINTNAFVENTQTTLAGAGTPGPAQTPGTLNDVRTVPGPSPGGRFNSGFPLWDGSGRI
                     LVSWSQCRLLDPQDPTRIIRCESDSAEDPDAV"
BASE COUNT         1626 a         2975 c         3148 g         1755 t
ORIGIN      
        1 cttgagctgc cagaaccagc cgacgcgatt gttggcgtcg cgctgcagga gtttgtcgag
       61 catcgtgccg tcgtcgatct cggcaatgga cgcatcgaga ttcacggtct cggtgtcgaa
      121 ggaacggatg gccagctcgt agttttcggc ggcctgcgcg ttggcgttga gcttggcgaa
      181 cgcgtaggga atggcgaggt aggcttcctg cacggcggag tcgagcaggt tgcgttcgcg
      241 cagttccatc cacggcgtga gcgcttgctg gaactggcct tcggccgcgt cggcccagcc
      301 cacgcctaac aacgccttgt tcgagaacgg tccgtcgagg cgcacgcgtt cgagcacggg
      361 cttggcctcc gccggctggt tgttctgcag gtaggcgtag cccaacgcga gattcgcgcg
      421 gtcgcgcagg ctgcgcagct cacggcgacc ggattcgagg gtgcccacgg cagtgaggaa
      481 gggatcggcc tcggtgacca ggcccttgcg caccagcgcg acgcccaggt tgtactgcgc
      541 gtaggccagc cagtcgctct ggtcctggcg gcccttccag ttgttgagca gctcgatggc
      601 ttcatcgaaa cgctgctggc gcatgaggat gttgctgagc aggtgcatgc gctcggcttc
      661 gaggtccggc gtgagccggc cctgcacttc cttgatggcg cgttcggaac ggtcgagata
      721 gccgcgcgcg taccagatct tgccgagata gaaccaggcg cgattgcgga cgccctcggg
      781 caccgactgg gtcagcaggg tttcgaaacg ctcgccggcc tcgttgtgca ggccgagcga
      841 tagatacagg ccgccgagca gcaggtcggt ctccgcatcg tgatgcggca tgcggcccca
      901 ttgccggtac gcctgcagac gcgtgatcgc gtcgaagtcc tggccctgga agtaataaaa
      961 gagtgcgtcg ccaaaatgca ggtcgcgcac caccgttgaa gagagcttgt ccacctcttt
     1021 cttggcggcc atggccggcg cgcacgccac gagcgcacca cagaccaggg caatgcggaa
     1081 aaacttcgac atcgcgagtc ttattcccaa tccttgatgg cgaactcggg ctgcgccttg
     1141 cgctggcggt cggtgatctt gagttcgagg tatttcgcgc ccacgccctt ctcgaacttg
     1201 agattggcgc cgcgcttgta gtcgaggttg ttggggccct tgccggcgaa gaacgccacc
     1261 agctggtgct cgcccacttt caggttgccc aggtacacgc gatgcacgcc gcccttgagc
     1321 agcgcctcgg cttcgcgcgg cgtgtacagg tagttagcga cttccttgtt gtcgattttc
     1381 agcgtgagcg aatcgagcgc gaagaactcg ccaatgtcca tggagacgaa caccgccacc
     1441 tgggtgttgg ccggaaacag cagctcttcc tcgaggacga acagctcctt gttcagatcg
     1501 acgacgtctt tcttgagatc ctgcaccgtc tcgtcgagtg cgcgcgtgtc ggccggcggc
     1561 tcgccggtgg cggaaacttc ggcaccggcg tccggagccg gggcggccgg atcggctgcc
     1621 gcagcatccg cggccggagc ctcggcgggc ggcactgcct gcggatcctg cgcgagggcc
     1681 ggcagcgcga gaatgatcag tgtcatccag gcgaagcgga atgtcttgag ttgcggcata
     1741 gaggttactc gtcggattat tcgtctagaa gcgtgcgcag ctcgcgcacg tacaaggctt
     1801 catcacgcga tctgaactcg cggtgtgcca cgcgcagcac ccccgcgcga tccacgaaca
     1861 ggagcatcgg caggcgatcg acactgaagt cgcgtcccgt ggtcttggcg gtggccacca
     1921 gcagctggaa gttcacgctc agcgcgcgcg cgaacttctc ggcgcgcggg gtgttgtcgt
     1981 cgagattgac gccgatgacc gtgagcccgg cgctgccgta ggtcctggcg atgcgatcga
     2041 gcgcctcgag ctgcgcgcgg caggtgttgc acgcgccgct ccagaagcta actacgacga
     2101 cttcaccgcg gtgctccgag aggcgcacgt tctggcccgt gaggtcgcgc gcgaccaggt
     2161 cgggcgccgg ctggccgagc agcacgtgtt tctcggccgc gatggccgcg ccgccaccca
     2221 ggcacaaact caggccgagc gcgcacgcga gcgcaaacgc agcaaaacgc gcgtgcaatg
     2281 cggccctggg ctccactaac aaacaagcgg tcaaggttgg ctcaaagtaa gggaacaccg
     2341 gcaagcaatt tccggattcg acgcaatctg acgcgcgaaa ccatcccata gcccacttaa
     2401 caatgtcaac gaagcaagcc actgtttgag aagcagtaac ggaatgccgc ggcgtacatg
     2461 agttagccaa aaaggacaag ggagcgtcgg caacacgcga catcgcggtg gcctacgctc
     2521 cctcgagact gatcggtctg gctcgaacgc ctagaccggg ttgctgaccg ttccgttgac
     2581 gagcggcgcg atcatcgtga ggctgtcgcg caatgcaacg ctgcccaggc cgttcgacgg
     2641 gaacaacgtc gggaagttgc cttcctcgcc gtgcagcgcc atgtagttga ggatcacggt
     2701 ttcgaccagc aggttgacgg cgttcgccgc cgggctggac gaggtgacca cgttgccgct
     2761 ggcgttgagt gagccgatct gcatgcgcgt agcgctgcgg agctgcggac gcccctgccc
     2821 cggcttgtac accagcacga acggcgcggc ggtcgactgg ttgtcactcg cccagtcgag
     2881 cttgccacga ccttcgacgg tgttgttcac catgccgttc gccgagagcg aaccgtcgct
     2941 gaacacgtag atcatgagcg gcttgcccat gcgcgccgcg tactcgagac aggcaccgat
     3001 gcagcggccc gcgcggaagt tgcgcgtctc gccggtgttg cggccctggc cgtgatagtc
     3061 gtaaccaccc atggtcaccg tgccggcgcc cgcgtagccg ttgatgacca tcttcatgat
     3121 ggccgcggcc ttgcggaatt caccgtcggc gttgtactcg gctgccgtga agatgggcgt
     3181 gatgccgcca ggctgcgaag cctgcgccac gatgtgcgga tccaggttcg ggttgagcgc
     3241 cgcgggcgta cggaagcgat cggcgagata cgtggacttg aggtaaccgc aacgcaccag
     3301 tttgtgcagg gcggcttcct gcgccgcgag cttggtcggc gactggttga ccttggcgtg
     3361 cgacaggcgg gtcatggact cgagcactgc gatggcatcg tcctggttgc tgaacaacgt
     3421 gccgagttcg ccggtatcga cgagacccgt gacgtcgctg gcgcggtcca ctttcgtggg
     3481 gcgcgcggcc ggattcatca tcgcggcggg cgccatcgag ttgccgcccg agtcggtgtt
     3541 ctgcgagccg atgagcgtca gcagctggcc gtcggcgccg acgcggttga tgccgtacat
     3601 cgggttgtgc gggttgttgc ccgtgtcgtt ttccgaccgc gccgcgatgg gaaagccatt
     3661 gacgttggcg cgcgtggccg caagcgtgga cgcctgcatg ccgcgaagga tggcgccgtc
     3721 cgaatgccag gcggcaccga actcggtatt gacgaagttg ttagtcgggc tggccgcatt
     3781 ggccgaaccc ggcgtcatgt tgcccggcag gccgagcgtg gcgtaaccct gcgtcgacag
     3841 gaagttcagc gggttgccgc tctgaccgca cagcatttcc gaaccggtga ggttcgcgcc
     3901 gccggcgaga tcgaaggcga tgaacgggat cttgcccgca ccggcctgga ttgcgcaggc
     3961 cgcgggtccg agcagcgctt cgagatcggg cgacatggcg cgggcgttgt cggaaccgat
     4021 gagcatcgcg agcgcggcag gcgcggcgat gatcgcggga ccggttttca tgccctgggc
     4081 gatgaagtcg cgccgcgtga ccggcgcctt gtggctctcg tgccggatcg gctcatcgag
     4141 acccaggagg cgcggactct tcttcgtgcg gatgatcatg acttcactcc cttactggat
     4201 cagcgtcgtg ccgctaccga gaacggcgcc gcacgctgct ttcattactg tgcccgtacc
     4261 attggccgcg ccgcccgtgg ggcccgcgac cagcttgtcg atcaggtcgt caatctcggc
     4321 cgggatcatt ccgtcgacgg tcgattccca ttcgaggccc gtgttcttgg ccttggtcat
     4381 cagcgccgtg atcacgaggt cgcggttcgc ggtgttcggc gaacttacgg agccgaaggt
     4441 cgaggtcgcg gaagcggccg gattcagcga gccaccgtag aaggcggtgc gcagcgccgg
     4501 tgtgtcgacc atctggctgc agtactggat cgcgagctgc gcgagtccgg tctgctgcgc
     4561 agggccgaag gcaccgagct tctcgatcga aggcagcgcc tgcttcacca gcgtgtacgt
     4621 gctgaccacg gccgcgttgt tcggcgacac gcccgtgatg agtgacaacg aggcgttgag
     4681 ctcatcgaag gtgcgcaggc cgacgtccga ttccgctggc agatccaccg gcgtcggagc
     4741 cgaaggcgcc ccggggggcg gcaacggatt gtcgttggcg ccgatgtgat cgaactgcag
     4801 gaagtacaga tcgtcgatcg gaccattctc gatcgacacg actgtgccga ccggggtcat
     4861 cagctgaccg gcacctgcgg tgtagttagc accgccgatc gtctggtcga gaggctcgta
     4921 agcctggccc gtgtgcagga tcgcgccgtt cacgccgacg cggacaccgc tgatctgcag
     4981 gttggcgacc gaggcgttcg gatcgaggct gatgaaggtc ggcttggaga actgcagacc
     5041 gtagctgtcg agctggctgg cctcgatcat cacgtacgcc tgcggcacgg tgaccaggtg
     5101 gctcacgttg aacagcatga agtacttctc gccgacgccg gcgtcgaagt tctgctggat
     5161 ctgcgacggc gacagcgcgc gattgtggat cgcgacgagg cgcagaacgc cttcccactg
     5221 gcggttggtc gaagtttcgt tgccgagcac cagcgcgaag gtgtcgtccc agtcggcgag
     5281 cgaaccgccg ccctgggcat cgaggtcacc ggtgtacacg ccgttcacat agatacgacg
     5341 gccttcgacc gggctgtaag tcatgaccac gtgctgcagc gccgcctggg cgtccatatc
     5401 tgccgcacgc gtgatcagca ccgggttgcc gttggcgctc gtcttgtcgc tgcgcgtgta
     5461 accatggtag gtgtactcgt tctgcgcgag cgtggcgttg cgcgcgttgg cgcccgccga
     5521 gtagctcacg atgaacgcat cttcctgggc gacgttggcg ttgttggccc aagcttcgat
     5581 ggtgaattca cccgtcgact tgatgaggtc ggcgagtttc ttcgagctgg ccgcagtgcc
     5641 ctgcgccttg ccgcccatct tgacgttgat gccccagccg ccgacccacg cgacatcacc
     5701 ggagagcgtc aggttgagtg ccggctcgtg accgctggtg tcataggcaa tgttgcccat
     5761 gccggtcttg aactcgaact tggcgatcgt ggccgcttcg taacggttgc cgcccgcggc
     5821 gaccgtaccg tcatacagcg tgagaccctt cgagatgagc atgcccgcgg gaatttcgtt
     5881 caccggtagc ggatcgacaa aagcattgat cgcctggagc atggtggcag cgttgtccgc
     5941 gcaagtcccg ccactggccc agcagttgtg cgactcgtcg cccagacgaa cgaccaggcg
     6001 tgaatccgca gtattggcga gattgatctt cgacttgatg gcggcatagg ccgtttcgat
     6061 cgacgcgggc gacggaaccg caccgccttc cgcgaagaac ggctgctgtt gcgcggccga
     6121 ggtcgaggag tggcagcgtc cacaatttgc ccgggcgggg tcgcgcacca gtgcgtatag
     6181 agcagaggaa gcgaacagcg ccgaagaagc cgggaagctc ttgctgccgc ccacttcttt
     6241 gatcgtcgga gcctggagct ggatctgagt gccaccggtg gcgctcgcgc cagcccagtt
     6301 gcggatccac actgtcagcg tgtcaccgca agccgcggga gactgcagcc agcaattgtg
     6361 accgccggcg accttcacga ccatgcgtga ctgatcgggc tgcgtgaggt tcacgacgcc
     6421 gttggctgcc tggtacgcga ggttcacgtc atcgttgcgg gcgaacatcg gagtctgacc
     6481 accggcgccg tggcacgaac cgcagcgatt gttgcccttg atgttctccc acaggttgat
     6541 gcggaaggcc tgcacgtcgg cgttggccga agcgggaccc gtgtagtcga ggaccggggg
     6601 cgccgtcgtg acggggttga ccaccgtcgg tgcgccgccg ctcgaacaac cagcaagaat
     6661 cgcggctgcc gtggctgccg agaaccaatg aagaattttg gactgcatag cgttcatgtg
     6721 tgtttcctca atcgcccatg cagtacacag cggtttcggc gaacacctgt ttcaaccgat
     6781 aaccggtgga ctggaagttg gccgtcatgg tcgcgacctg ggcgcgatcg gctgagttgc
     6841 ccggcgcgcg gaagcacacc gtcctgaaca ccttctcgac ctggcattga gcgaaagcgt
     6901 cactgttggc gagttcctcg cccatggact tcgcaccatt gccccggccg ggcgtcgcgc
     6961 cccacccgag cagggagttc tggcccacgc gccagcggtt ctcccaggcg tcatcgggag
     7021 tgatgaagcc cggtgcgaag ttcgtgttgt tgttgaagta cttcgaatgc acggccgtgg
     7081 agttgtagac caggcgcatg gtgcccgggg cttcctccga gccgttgtcg aagtcgtagt
     7141 acgcgaacgc ctgcgcgaac gggtccatgc cgctgtggca gccgatgcag ttgttcagga
     7201 agatgcggct gtcgccaccg gggctgcggc tcacgtcctg gcggatgcga tccgggggca
     7261 gcgaggtgtc gtgcacctgt tccatgtcgc ggcacatgtg attcatcatc gtgaagcgga
     7321 acatcgcgcg gttggtgccg tcgacgaaga acgcttccga ggcggcgcgc gaagtgatga
     7381 acccggcggt ggccgcgggc gggatgccct ggatcgacga ctgcgtggtc ggcgtcagcg
     7441 cggtgaacag gttgacgttg ttcgtctcgg cgttctcgta gtgcgtgttg ttgctggccg
     7501 aagcggccgg cgtcgaaccg ttcacggtgt aagtgaggtc ggccgagagc gcggtgttga
     7561 acgcgacgtc gtcacgcacc attccaatga aggtggcgac gtagtcgttg agcggcgcga
     7621 acaccgtctg gtcacggttg gtccacggca ctgccatgtt cttgagggtg acgttataga
     7681 aggacggcgc ctgggtggcg atgttggccg cgccaatcgc attgccgccg gcgaccatgc
     7741 cctccatcgt gtccagggtc gcctcgctcg gcggaacgcc ggcgatgcga tcgtagatgc
     7801 gcttggcctg atcgcgatcg ttcgccgacg cggcaattgc cacgctcgcg agcagaaccg
     7861 caaggccgac cgtcttaact tttgaaacct gtggggtatt catttgcctc atccacatcc
     7921 cttttgacga acgcagtatc acccggcccg ccaacacact tgtttgtctc cgctcctcta
     7981 cgtctccaag cggcgacagc accatttatt acgtacgcac agtagtcttg ggcccgagga
     8041 ttgcgaaccg cgtcacggcg cctgccgccc cgtagcccta actaaacagc tgacaagaaa
     8101 accgctttgc ggctctgtct gctgtgacgt tgtcggcaga tcgatgtggc ctcaagcggg
     8161 atgattgcat tacgtcacat gcctgtgcgg caatgcacat gcggccaggt gtggacgaaa
     8221 gtggaggatg ctgtgaatcg accatacgaa aacgcgaacc ggatcacgct cggacgattg
     8281 ggcttgggcc tggtcctggg cgcagccggc ttgttagcgg cctgtaccca gggccccggt
     8341 ggcggtggcg tcggcctcgc cagcggccag gacccggatc cggccacggt cgacttcccg
     8401 atcttctaca tccgccacca gattcccgaa gaccaggacg acgtcacgcg cctccggccg
     8461 ttcgtcgagg atgacgacta ttccgccacg ctctggaagc gcgaccgcgc ttcgccgggc
     8521 gcccccgaag ccgaaatcac cgcgcgcctg cgcgtcgatc cacttggttc accggaagac
     8581 cgctacgaca tcaaggacct cgccgtctcg cccgacggcc tgcgcgtggc gttcgccatg
     8641 cgcggcccgt tggacgattt cgacgaggac gaaccgccga cctggaacat ctgggaatac
     8701 aacatcgcca ccgacacgct gcaccgcgtg atcacctcgg acatcatcga ggaagaaggc
     8761 caggacgtct ccccctccta tctacccgac ggccgcatcc tgttctcctc gacgcgccag
     8821 cgccaggcca aggccatcct gctcgacgag ggcaaggccc agttcgaagc ggcaaccgaa
     8881 gcgcgcaacg aatccgcgtt cgtggtgcac gtgatgaatg ccgacggcac cggcatccac
     8941 cagatttcct tcaacccgag cagcgatctc tacccgcagg tgctgcagaa cggacgcgtg
     9001 ttgttcacgc gctgggaccg cgcaccgggc cgcgacggat tgcacctgta cacctcgaac
     9061 cccgatggca ccgacatgca gctgcactac ggtgcggtgt cgcatctgac gggcacggta
     9121 gtcgctggcg tcgccactcc catcgaattc atgcgcgcgc gcgaaatgag cgacggacgg
     9181 ctcatggtgc tcacgcgtca gcgcaccaac gtcgatttcg gcggcaacct gaccatcatc
     9241 aacacgaacg ccttcgtcga gaacacccag accacgctcg cgggcgccgg cactcccggc
     9301 cccgcgcaga ctccgggcac gttgaacgac gtgcgcacgg tgcccggtcc ttcgcccggt
     9361 ggccgtttca attccggctt tccgttgtgg gacggctccg gccgcatcct ggtgagctgg
     9421 tcgcagtgcc gtctgctcga tccgcaggat ccgacgcgca tcatccggtg cgagtcggac
     9481 agcgccgaag acccggacgc ggtc
//
LOCUS       DASQJS010000333         4894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_832157, whole genome shotgun sequence.
ACCESSION   DASQJS010000333 DASQJS010000000
VERSION     DASQJS010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832157"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="VEW08_13340"
     CDS             <1..1393
                     /locus_tag="VEW08_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HYJ41770.1"
                     /translation="AIKERQTAAKPWLGDLDGMLERRVVRMLVVYNKTNYFLDGPQQR
                     GATYDMGVELEKWLNRTNKDKTRAIRVVFIPTARDHLLTDLQAGRGDIAAAGLAITPE
                     RQQLVSFARPFIDDVSEILVTSAEGAVPATVEGLSGQSVYVRKSSSYYSSLESLNTRL
                     KSQGKPPVIIVPADENLEDEDILEMMNAGIIDSTVVFSGLATFWQQIFTNIHPHPELV
                     LRARSEIAWAIRKESPQLKASLDAFVDKNKSGTMMGNMVLRRYLQNTRWARNATSAAD
                     MKRFDELSGYFKKYSEQYKFEWLLLVAQGYQESGLDQSIRSPVGAIGVMQVMPTTAGD
                     RNVAIKDIHLVEPNIQAGAKYMRFLVNQYFDEPGIDNLNRHLFAFAAYNGGPNRIARL
                     RREAAARGLDPNKWFNNVELLAAEQIGRETVQYVSNIYKYFLAYKLVQERGEQRDAAK
                     AKATASQAPTKTQ"
     gene            1454..2407
                     /locus_tag="VEW08_13345"
     CDS             1454..2407
                     /locus_tag="VEW08_13345"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ41771.1"
                     /translation="MAGPSRQNALIDTDAESLLQPFPGWQAEWIHLGPAPASYHGVRV
                     SFESGTFTALHTSSAGILRGNTSADFVAMLTSAPASAYPRNCSHAIGGDRCLVLGPAA
                     NLDLYLPQGCNLMIMALPVPPSNGGSTLTTGLSQYCELDKAQTNLLSRCMDSLEEIRM
                     EPFSSQATRVAQRRLRDLLSPASAMVRDTSPRPYLQRARLQRHLAVIRACEFVDSHLR
                     APIALVDLCEAAGVSTRALEYGFRDFYGLGPMAYVRNLRLCRVRHDLLDHADSVSSAA
                     RRWSFTHMGQFSHDYRALFGEVPSKTLAANQRAAKATGPVS"
     gene            complement(2371..3333)
                     /locus_tag="VEW08_13350"
     CDS             complement(2371..3333)
                     /locus_tag="VEW08_13350"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ41772.1"
                     /translation="MREIGLKSAGLGATGDLAGCLTWDIAQLATGQLEVERDFVPFDG
                     AWVGSVNVDRTALHQLHTREGWGAVLLPAPESGAVYFEGRRLVAGDAVLLPPDSDLDL
                     LTHAVGRVYLIAFRGGAGSRVRLRRPRWYTSADGCAGCGFSEDLRALLASVNAGDAHL
                     AAALQVRLLSWLRAALADAERPDDQVTVLPRRRMAVERVRRFIHEHLAETMTLAELCR
                     HAHLQARSLEYGFRDLVGLSPFKYIKMLRLGEVRRRLQTSSAAERSVSEVALDCGFCH
                     LSQFAADYKRVFLERPSATRRASECERPGRQLRYETGPVAFAAR"
     gene            complement(3462..>4894)
                     /locus_tag="VEW08_13355"
     CDS             complement(3462..>4894)
                     /locus_tag="VEW08_13355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYJ41773.1"
                     /translation="GTDGGCEFVNNVWLDFTGRALSQARGYGWVQSVHADDQKRCVAI
                     YQSSFSARCEFEMDYRLRRRDGVYRWILARGVPRYGATGEFVGFIGSAIDVTDRRRQE
                     SALRQSEERYRAVVDSQTELVCRFAPDLTLTFVNEAYCRFLGKQRDEVLGKNLGAYFP
                     ADTREQLARSVTHVLSGAGQAELQCEVTLPDGGRGWQHWAFKSIDGAGEIPEELQAIG
                     HDITDRKRADDVQRRLAHSSRLAAVGELTAMVAHEVNQPLCAILSNAEAAETLLSREN
                     PPLQEIREIIRDIRDDDLRADEVIRGIRSLVGKREIRIQPVDLNRMVTHVLRLVSGDA
                     LFRRVRIQRMLDESLPLVAADQPQIEQVLINLVVNGMDAMQATPEAARELSVHTRLVS
                     RDLVEVAVVDHGSGIPPEKLPELFDSFFTTKPDGMGVGLSIARWIVLAHGGRIWAVNG
                     AHGGAEFRFTLRTAADTTNTAADLTT"
BASE COUNT          952 a         1656 c         1448 g          838 t
ORIGIN      
        1 tgccatcaag gagcgccaga ccgccgccaa accatggttg ggcgacctcg atggaatgct
       61 ggagcgacgc gtggttcgca tgctcgtggt ctacaacaag accaactact tcctggacgg
      121 tccacagcaa cgcggcgcca cctacgacat gggcgttgag ctcgagaaat ggctcaatcg
      181 caccaacaag gacaagacgc gggcgatacg cgtggtgttc attcccaccg cgcgggatca
      241 tctgctcacg gatcttcagg caggacgcgg cgacatcgct gccgcgggcc tcgcgatcac
      301 gcccgaacgc cagcagctcg tgagttttgc caggcccttc atcgatgacg tcagcgaaat
      361 cctcgtcact tctgccgagg gagcagtacc ggccaccgtg gagggcctct cgggacaaag
      421 cgtctacgtg cgcaaatcga gcagctacta ctcgagcctc gaatcgctca acacacggct
      481 caagtcgcaa ggcaagccgc cagtcattat cgtccctgcc gacgagaacc tcgaagacga
      541 agacatcctc gagatgatga acgccgggat catcgacagc accgtggtct tcagcggact
      601 ggctacattc tggcagcaga tcttcaccaa catccacccg cacccggaat tggtcctgcg
      661 tgcccgcagt gaaatcgcct gggcgattcg caaggagtct ccgcagctca aagcttcgct
      721 cgacgcgttc gtcgacaaga acaaatccgg aaccatgatg ggcaacatgg tcctgcggcg
      781 ttacctgcag aacaccaggt gggcgcgcaa cgcgacctcg gccgcggata tgaagcgctt
      841 cgacgaatta tccgggtact tcaagaaata ctccgagcag tacaagttcg agtggctgct
      901 gctggtagcg cagggatacc aggaatccgg cctcgaccag agcatccgca gcccggtcgg
      961 cgccatcggc gtcatgcagg tgatgcccac caccgccggg gaccgcaatg tcgccatcaa
     1021 ggacatccac ctggtggagc ccaatatcca ggcgggcgcc aagtacatgc gcttcctcgt
     1081 caatcagtac ttcgacgagc caggcatcga caacctgaac cgccacctgt ttgcgttcgc
     1141 ggcctacaac ggtgggccca atcggattgc aaggttgcgg cgggaagccg ccgcgagagg
     1201 tctcgacccg aacaagtggt tcaacaatgt ggagctactt gccgcggaac agatcggccg
     1261 ggaaaccgtc cagtacgtga gcaatatcta caaatacttc ctcgcctaca aactggtcca
     1321 ggagcgcggc gagcagcgcg atgccgccaa ggccaaggca accgcttcgc aggcgccgac
     1381 gaaaacgcaa taaagcggtg caaatggcgt tttcttgata gtcaggagcc acgacagcga
     1441 ttccactagc acggtggctg gcccttcacg acaaaacgcc ctgatcgata ccgacgcgga
     1501 atcgctcctg cagccgttcc cgggctggca ggcggaatgg attcacctgg ggcccgcgcc
     1561 ggcgagttat cacggcgtac gcgtctcgtt cgaatcggga accttcaccg ccctgcacac
     1621 ctcatccgcg ggcatcctgc gcggcaacac cagcgccgac ttcgttgcga tgctcacgag
     1681 tgccccggct tcggcatatc cgcgcaattg ctcacatgcc atcggcggcg accgctgcct
     1741 ggtgctcggc ccggcagcga acctcgacct ctatctaccg cagggttgca acctgatgat
     1801 catggccttg cccgtgccgc cctcgaacgg cggcagcacg ctgaccaccg ggctctcgca
     1861 atactgcgag ctcgacaagg cgcaaacgaa cctgctgtcg cgctgcatgg actcgctgga
     1921 agagatccgc atggaaccct tctcgtcgca ggcgacccgc gttgcccagc gccggctgcg
     1981 tgatctgttg agtccggcgt cggcgatggt gcgcgacacc agtccacgac catacctgca
     2041 acgcgcgcga ttgcagcgtc atctcgccgt cattcgcgcc tgtgagttcg tcgactcgca
     2101 tctgcgcgca ccgatcgcgc tcgtggacct gtgcgaggcg gccggtgtga gcacgcgcgc
     2161 gctcgagtac ggattccggg acttctacgg cctggggccc atggcctacg tgcgcaacct
     2221 gcggttatgc cgcgtgcgcc atgacctgct cgatcatgcc gattcggtca gcagcgcagc
     2281 acggcgctgg agcttcacgc acatgggcca gttcagccac gactatcgag cgttgttcgg
     2341 cgaggtgccg tccaagactc tggcggcgaa tcagcgggcg gcaaaggcaa ccggccccgt
     2401 ttcgtagcgc aactggcgtc cgggccgctc gcattcgctc gcgcgacgtg ttgccgacgg
     2461 gcgctcgaga aacactcgct tgtagtcagc ggcgaactga ctgaggtgac agaagccgca
     2521 gtcgagcgcc acttcggaga cactccgttc cgcagcgctg ctcgtctgca gtcggcggcg
     2581 aacttcgccc agccgcagca tcttgatgta tttgaacggt gacaggccga cgagatcacg
     2641 aaatccatat tcgagcgaac gcgcctgaag gtgcgcgtga cgacataact ctgccaacgt
     2701 catggtttca gcgaggtgct cgtggatgaa gcggcgcact cgttccaccg ccatacggcg
     2761 ccgcggcagc accgtcacct ggtcatcggg acgctccgca tccgccagtg cggctcgcag
     2821 ccatgaaagc aaacgcacct gcagggcggc tgcgagatgt gcatctcctg cgttcacgct
     2881 ggctaacagg gcgcgcaggt cttccgaaaa tccgcagccc gcgcaaccat cggcgctcgt
     2941 gtaccagcgt ggacggcgga ggcgcacgcg cgatcccgcg ccgcctcgaa aggcgatgag
     3001 gtagacccga cccaccgcat gcgtaagcaa atcgagatcg ctgtcgggcg gcaacagcac
     3061 cgcatcgccc gccaccaggc ggcggccttc gaagtagacg gcgccggatt ccggcgctgg
     3121 cagcagcacg gcaccccaac cttccctcgt gtggagctgg tgcagcgctg tcctgtccac
     3181 gttcacgctg cctacccagg ctccatcgaa tggcacgaaa tcgcgctcga cttcgagctg
     3241 cccggtggcc agttgcgcaa tatcccaggt caggcagcca gccagatccc cggttgcacc
     3301 gagcccggca gatttgagtc caatttcgcg catcgccgcc gtctgctccc gagggcgtcg
     3361 cgccttggca cgacgatgcg tctttttatg acacgccgcg gcacgccaac atcggacctt
     3421 ggtcctacag cccggaagcc cgccaggcgc ccgctttcgc gctatgtagt tagatcggcg
     3481 gcggtgttcg tggtgtctgc cgccgtgcgc aaagtgaacc ggaactccgc gccaccgtgc
     3541 gcaccattca ccgcccagat gcgtccgcca tgcgcaagca cgatccagcg ggcgatggag
     3601 agccccaccc ccatgccatc gggcttcgtg gtgaagaagg agtcgaacaa ctcgggcaac
     3661 ttttcgggcg ggatgccgct gccatgatcc acgaccgcga cctcgacgag gtcccggctg
     3721 accaggcgcg tgtgcactga caactctcgc gccgcttcgg gcgtcgcctg catggcatcc
     3781 attccattga cgaccaggtt gatcagcacc tgctcgatct gtggctgatc agccgcgacc
     3841 agcggcaacg attcgtccag catccgctga atgcgcacgc gccggaacag cgcatcgccg
     3901 gacacgagcc gcagcacatg cgtgaccatg cggttcagat ccacgggctg aatccggatt
     3961 tcgcgtttcc ccaccagcga gcgaataccg cgaatcacct cgtcggcacg caggtcgtcg
     4021 tcgcgaatat ccctgatgat ttcgcgaatc tcctggagcg ggggattctc gcgagacagc
     4081 aacgtctcgg ccgcctccgc attgctgagg atggcgcaca gcggttgatt cacctcatgc
     4141 gccaccatgg cggtgagctc acccaccgcc gccagccgcg acgaatgcgc cagccggcgt
     4201 tgcacatcgt ccgcccgctt gcgatcggtg atgtcgtgcc cgatggcttg caactcctcc
     4261 ggaatttctc ccgctccgtc gatggacttg aaggcccagt gttgccagcc gcggcctcca
     4321 tccggcaagg tcacttcgca ttgcaattcc gcctgccccg ctcccgacag gacgtgcgtc
     4381 acggaccgcg cgagctgctc gcgggtgtca gccggaaaat aggcgcccag gttcttgccc
     4441 agcacttcgt cgcgctgctt gccgagaaac cggcaatacg cctcgttgac gaaagtcagc
     4501 gtcaggtccg gcgcaaaacg gcacacgagc tccgtctggc tgtcgacaac cgcgcgatat
     4561 cgctcttcgc tctggcgcag cgccgattcc tgccgtcggc ggtccgtgac gtcgatcgcc
     4621 gacccgatga aacccacgaa ctcgcccgtg gcgccgtacc gcggtacgcc gcgagccagg
     4681 atccagcggt acacgccgtc acggcgccgc aatcggtaat ccatttcgaa ttcacagcgt
     4741 gccgagaagc tggactggta gatagccacg cagcgcttct gatcatcggc atgcactgat
     4801 tggacccagc cataacctcg tgcctgcgat agtgcccggc ccgtgaaatc gagccagaca
     4861 ttgttgacga actcgcagcc cccatccgtt cccg
//
LOCUS       DASQJS010000334        13098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2090879, whole genome shotgun sequence.
ACCESSION   DASQJS010000334 DASQJS010000000
VERSION     DASQJS010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2090879"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VEW08_13360"
     CDS             <1..895
                     /locus_tag="VEW08_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYJ41774.1"
                     /translation="VELFHAISSPVFQDLQPLTGTSLADLRRESLDMLQKRLEASAER
                     ADARDVTVNCHVTWDYPPHEAIVRRASRTGADLIIAEYHDGSRLKPWLLHLTDWELLR
                     LSPVPVLLLRDTRKYLRPAVLAAVDPSRTHEKPAKLDLEIMAAGELLADSLQGSLHAM
                     HANYPMLTGLKLGDTVFDAGALAESYRDQKRQDSAAFRAFADKAGIPRAKRHELDGDP
                     VYAIPKAAAATRAQIVVMGAVSRSGLKRVFIGNTAERVLNALPCDVLVVKPPRFEKKR
                     VQKASRGMRVISATPLVSVSV"
     gene            complement(898..2241)
                     /locus_tag="VEW08_13365"
     CDS             complement(898..2241)
                     /locus_tag="VEW08_13365"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HYJ41775.1"
                     /translation="MRFNPLVRVLLPCALIVSVAVVACVSVGPTDTGPVESPQTSVGP
                     NPSLPEPEKQLIPIVQVAEAKGWPAGSKPAPATGTAVNAFASGLVHPRWLYVLPNGDV
                     LVAETNAPVRPDEGKGIKGKVMKKMQGKAGAGVPSANRITLLRDVDGDGVAEIHTPLL
                     ENLHSPFGMALIGSELYVANTDAIVKFPYSAGTTKISAAGTKVADLPGGTRNHHWTKN
                     IIANKSGTKLYATVGSNSNVAEHGMQEEEGRAAIWEVDVKTGAKRLFATGLRNPNGMA
                     WEPKTGTLYTVANERDELGNDLVPDYLTSVKDGSFYGWPYSYYGQHVDTRVEPAQPDL
                     VAKAVSPDYALGAHVAALGLTYSDGKGMPKHLSNGMFIGEHGSWNRKPPAGYKVVFVP
                     FVNGKPSGMPLDVLTGFVSKDGVAWGRPVGVAIDKRGALLVADDVGNIIWRVTGR"
     gene            2412..2825
                     /locus_tag="VEW08_13370"
     CDS             2412..2825
                     /locus_tag="VEW08_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ41776.1"
                     /translation="MLRTILVYGVLLAAGAFGLQWLEYHFVVRTHATETYLVLVALAF
                     MGLGIWVGAQLFRRRPSAPFEPNLKVRETLGVSDREFEVLTLLAAGRSNKEIASQLNV
                     SPNTIKTHVAKLFEKLEARRRTEAISRARELGMIR"
     gene            2927..3469
                     /locus_tag="VEW08_13375"
     CDS             2927..3469
                     /locus_tag="VEW08_13375"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HYJ41777.1"
                     /translation="MTAIILRYGVIAGLIIGVPMLWRMLSAKPGEVPVVGMLLTYLVM
                     IVALSAVFIGVKAYRDKVLGGVVRFLPALGVGLGISAVASIVYALAWEISSAYSSFDF
                     VAFYKDYMVDAAKGGSPAEVNQAIANAEAFEKMYRNPLYRIPMVFVEMFPVGVLISLI
                     SAAVLRNPRVLPARGAAPIS"
     gene            complement(3489..5759)
                     /locus_tag="VEW08_13380"
     CDS             complement(3489..5759)
                     /locus_tag="VEW08_13380"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYJ41778.1"
                     /translation="MKSIAKLRGWIGESVSRRLAIPGLVLILLLLVQAALSSGSAWVL
                     VGRLEDSSTNSNASMELTGRLLNAAQELSDHARETVAATDDEQRNKGIAAFNEAKARL
                     GEVVDEISTKLSSDPSLQQAVSDGLSTFVVSGVKATRLAQRGRVEDAQRELQVTFDPN
                     LLAYVISTVTALNQTSAGSLSGVLKAGRIDFWIALGVAAVLVLIAAFAAFRAFRAVRN
                     AVIEPVQYAALTARRLATGDYDEVRKSDDPAECGELVRAMSDLCQQLIERRTAAESAA
                     SAAVGAFRVRSGLDLASSRVLITDPDGRIIYANAAARSLMDLIQPGTAIVGRQLDDLL
                     SAASANEGAASGDPRSTRLRYLQTVTDVVVSTITTEKGELLGRVAEWVDRTDEVRAQR
                     EVSAVVHAAGLGDFSQRIPGDGKSGYWGELASSLNQLTEMFHTALHATSLQLAALSQG
                     ELTETAGHRYQGLLAQVFDDLAASRSHLSRMVSQIRTGSENVSSVAATINEGNETLHT
                     QGEELTHSIAAAVQNMKEITDAVRRNADNALRVRTIATEARDAAVRGGLVVGNVVKSM
                     AEVSATSARVVDVVSVIDEIAFSTNLLSLNAAVEAAHAGEHGRGFAVVAAEVRSLSQR
                     CTASAREIKELIEASNLTVRQGTALVTSAGKTIEDVVERVATMADVVGEIARDAQEQC
                     VGIETVARKVAEVETSNSENFSLLARARESAHELQMLAGELGGAVGRFRIAEKGTDLF
                     SASPVVELARPSIRGK"
     gene            complement(5772..6173)
                     /locus_tag="VEW08_13385"
     CDS             complement(5772..6173)
                     /locus_tag="VEW08_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYJ41779.1"
                     /translation="MKLAVSAALLLMLAAPLGAPTAWAEDDAGPLPRDTADASVIRGG
                     IAFRTYCVLCHGAAGKGDGRAAKMYTPKPANLTVSPFPDQYKEMIIRGGGVSVGRSAF
                     MPPWGDELTNEQIHDLVAFLRELRKTGTPAN"
     gene            complement(6170..6475)
                     /locus_tag="VEW08_13390"
     CDS             complement(6170..6475)
                     /locus_tag="VEW08_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HYJ41780.1"
                     /translation="MSLLAVVMTCAMPAFAGDQTVGQAKKAFTVGVLKAKVGDTITFH
                     NDDEFAHNVFSLSDAQSFDLGSYKKGETRNVKLTAPGTIEVECAIHPEMKMKIEVAK"
     gene            complement(6523..7539)
                     /locus_tag="VEW08_13395"
     CDS             complement(6523..7539)
                     /locus_tag="VEW08_13395"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HYJ41781.1"
                     /translation="MSHKFISQRVSLPLTLLFGFLLIGSVVASESDPWLRPEFAPAPP
                     ENQLTPERVHLGQLLFFDPRLSRKSTMSCASCHNPSLGWSDGLPTAVGFDMHTLARAT
                     PTIVNTSFSPIQMWDGRKSTLEDQATGPIEAAGEMNLPLPELIERLKSIPGYASKFEA
                     AYPGMGITAVTVARGLASFERTVLSTESPFDRWRKGDQKAVSASAKHGFELFQDKAKC
                     AVCHMGYNFTDNGFHNIGLATSGAPDVGRFAQRPLKSMHGAFKTPTLRDIGLTAPYMR
                     NGSYKTLMEVVEHYDRGGDDKTNLDINMVPLSLTASEKADLVAFLESLTGVPADVRLP
                     ALPQ"
     gene            complement(7696..8658)
                     /locus_tag="VEW08_13400"
     CDS             complement(7696..8658)
                     /locus_tag="VEW08_13400"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HYJ41782.1"
                     /translation="MKPAMLGLAVLLAQTSFATTLTVGNPKTNDYQSVQAAVDALPES
                     GGMIVIGAGTYREKLEIAKPNVKLIGKSKGNRPENVVLVWGDSNKSAGGTGKSASVWA
                     SGDGFEAHNLTIQNDYHLGNTERSQAVALRLTADRAVLTNVRLLGAQDTLYAASKNPD
                     SPSRQYFRDCYVEGHVDFIFGDAKAFFDRCHIHGIANDIVMITAQSKVKPGQDSAYVF
                     DRCRITADAGVKELWLGRAWRPYATVVFMRTRIDAPLQPAGWREWTPGTTETFKTATY
                     REFRSTGQGASIATREPRAVQLTKSETKAWSLKNFLAGSDGWNP"
     gene            8773..10206
                     /locus_tag="VEW08_13405"
     CDS             8773..10206
                     /locus_tag="VEW08_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HYJ41783.1"
                     /translation="MSQAKSPVTNSRRAFLRNVGVGTSALGVLGPGALLIPSVSQAIC
                     EPPGTPGSARPWRADCRPIRARRPASTLSAAEIQKLKDAYKAMRDLAVSDPSDPRGFT
                     HQANIHCWFCSVSPTPVHGNWQFFAWHRAYLYFHERILGKLINDSEFRLPYWDWEIST
                     HRQMPGAYTTPNNNSNPLWNSTRAMSPTDELPDEDVNEDVMEPVYSAANFGEFGGGAG
                     YSGIPEGSPHGSVHVDVGGFAGDMGAFDTAGRDPIFYAHHANVDKIWSDWNKGASSHE
                     NPTDPAFLNLSWSFYDENRIWRSIKASQLLNHETQLRYNYGLSRFREILPCILDWIIV
                     RPPWKFDRPVIVEPRFRDQITKALDGRRPVRLHINSPQIPLDKSAVYRVYANLESAAA
                     DEGPKSPAFLGVIPFVLNNREKHHPSKAPQVVLNVSRRISALLHSTDGIQLAYVERPA
                     KRAKPSGKAQLLTAREVYFSYGETMRE"
     gene            10199..10684
                     /locus_tag="VEW08_13410"
     CDS             10199..10684
                     /locus_tag="VEW08_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41784.1"
                     /translation="MNKFWWVSLSVALGLVAMSALGAEPVESSPGSTKEFVVAMPPDI
                     GSARSVILVLSDVRLPRMAAVVLRARLVETKAVKGGADIVALLPLGSVGLLAESANAQ
                     GVTHHSVVRIEVTKKLKRWRQDHPGVSSLNIRVIPFAGADPLPHLEWSAASARLSLSG
                     S"
     gene            complement(10681..11805)
                     /locus_tag="VEW08_13415"
     CDS             complement(10681..11805)
                     /locus_tag="VEW08_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYJ41785.1"
                     /translation="MRAAAFFFVILLFTVGAGAADGPYVLGDGAGWQGVTVEEAAGVP
                     RKRALPLAAGASITVKAVGNLPAFQVKLRGPATPAPDEIRTSGKAPLFVVADTHGEYE
                     ILVAMLQKHGVVNAQLGWTFGRGDLVVLGDVFDRGPNHTEILWLLYQLEAEARRAGGG
                     VHLVLGNHETMVLLGDLRYLNPKYVQTAQLLGVGSYSELFGATSVLGQWLRTKPTMLK
                     INDLLCLHAGISRALIDSGLSLREINAGVRSLLDGSMPTNDAQRERAALLMGSTGPLW
                     YRGYFAEQTTFPTATMADVDLALEKFGAHRILIGHTIVPTVRSLYDGKVIAVNVYPRR
                     DDDGRANFESLLIRRDGTWRAKVDGTLEVIPGAAQPKRPP"
     gene            11855..12757
                     /locus_tag="VEW08_13420"
     CDS             11855..12757
                     /locus_tag="VEW08_13420"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ41786.1"
                     /translation="MSRFLIALMLLPVVGYAQAPAVESAKAVLVTGASTGIGRKITEH
                     LAGKGYFVYAGARKQADLDALNAIRNVQAVKLDVTRQDDIDAAVASITKAGRGLYGLV
                     NNAGVATVASATETSVEEFTLTMEVNLFGPWRVTRAFAPLIIANKGRITTIGSISGIL
                     APRDLTAYAMSKHAMEAFADSVAVQMAPLGVQVSIVEPGNFDSDIGKSATKRTGKESR
                     FTDRSKYKKPDEVAAAVEQALFEATPKRRYMVTPDQREAEITIRKQIEQLVQLNEGHP
                     YTYDRDALIRMLDESLATARPKTK"
     gene            complement(12767..>13098)
                     /locus_tag="VEW08_13425"
     CDS             complement(12767..>13098)
                     /locus_tag="VEW08_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ41787.1"
                     /translation="SLRDALEADGHTIVTANGGREGIETFREAHEAQQVFSAVVTDLG
                     MPYVDGRQVANAVKTISSTSPVILLTGWGQRLQAEGDLPAHVDLVLAKPPRLRELREA
                     LARCCAK"
BASE COUNT         2345 a         4267 c         4116 g         2370 t
ORIGIN      
        1 cgtcgagtta ttccatgcga tctcctcgcc ggtcttccag gatctgcagc cactgaccgg
       61 cacgtcgctc gcggaccttc ggcgcgaatc cctggacatg ctccagaagc ggctggaagc
      121 gagcgccgag cgcgcggatg cccgcgacgt caccgtgaat tgccacgtca cctgggacta
      181 tccacctcac gaggccatcg tgcgacgagc ctcgcggacc ggcgcggacc tcatcattgc
      241 cgaataccat gacgggagcc gcctcaaacc ctggctcctg cacctcaccg actgggaact
      301 gctgcgcctg agtccggtgc ctgtcctgtt gctccgggac actcgcaagt acctgcgccc
      361 ggctgtgctc gccgccgtcg atccttcgcg cacacatgaa aagccagcaa agctcgacct
      421 ggaaatcatg gccgctggcg agctgctggc ggattcgctg cagggatcgt tgcatgcgat
      481 gcatgccaac taccccatgc tcaccggcct gaagctcggc gatacggtgt tcgacgccgg
      541 ggcgctcgcc gagagttacc gggatcagaa gcgccaagac agcgcggcat tcagggcatt
      601 tgccgacaag gcaggcattc cacgcgcgaa gcgccacgag ctggatggcg acccggtgta
      661 cgccatcccg aaggccgccg ccgcgacgcg cgcgcagatc gtggtgatgg gcgcggtgtc
      721 acgctcgggg ctcaagcgcg tgttcatcgg caacaccgcc gagcgcgtgc tcaatgccct
      781 tccctgtgac gtgctggtcg tcaaaccgcc gcggttcgaa aagaagcgcg tgcagaaagc
      841 atcccgcgga atgcgcgtga tctccgcgac accgctcgtc tccgtatctg tctgaaacta
      901 ccggccggtg acgcgccaga tgatattgcc gacatcgtcc gccacgagca gcgcgccacg
      961 cttatcgatg gccacaccca cgggccgtcc ccatgccacg ccgtccttgc tcacgaatcc
     1021 cgtgagcacg tcgagcggca tgcccgacgg cttgccgttg acgaacggaa cgaagaccac
     1081 cttgtagccc gccggcggct tgcgattcca ggagccgtgt tcgccgatga acatgccgtt
     1141 ggacagatgc ttcggcatcc ccttgccgtc ggagtacgtg agaccgagcg ccgccacgtg
     1201 cgcgccgagc gcgtagtcgg gcgacacggc cttcgccacc agatccggct gcgccggctc
     1261 gacgcgcgtg tccacgtgtt gtccgtagta agaatagggc cagccgtaga acgacccgtc
     1321 cttcaccgag gtcaggtagt ccggcaccag atcgttgccg agctcatcgc gctcgttggc
     1381 cacggtatac aaggtgccgg ttttcggttc ccatgccatg ccgttgggat tgcgcagtcc
     1441 cgtggcaaac aatcttttcg ctccggtctt cacatcgact tcccagatgg cggcgcggcc
     1501 ctcttcctcc tgcatgccat gttcggccac gttgctgttc gagcccactg tggcgtagag
     1561 cttcgtcccg ctcttgtttg cgatgatgtt cttggtccag tgatggttgc gcgtgccacc
     1621 cggcaaatcg gcgaccttgg tgcccgcggc ggaaattttc gtggtgccgg ccgagtacgg
     1681 aaacttcacg atggcatcgg tgtttgccac ataaagctcg gaaccgatca gagccatgcc
     1741 aaatggcgag tgcaggttct cgagcagcgg tgtgtggatc tcggccacgc cgtcgccatc
     1801 gacatcgcgc aacaacgtga tgcgattggc ggagggcacg cccgcgcccg ccttgccctg
     1861 catcttcttc atgaccttgc ccttgatgcc cttgccttcg tcgggccgca cgggggcgtt
     1921 ggtctccgca acgagcacat cgccattggg cagcacgtac aaccagcgcg gatgcacgag
     1981 tccgctggcg aacgcgttga ccgcggtccc ggtcgccggt gcgggtttcg atcccgcggg
     2041 ccagcccttg gcttcagcca cctggacgat cggaatcagc tgcttttcgg gttccggaag
     2101 actgggattg ggccccacgc tggtctgcgg ggattcaacc ggtccggtgt cggtgggccc
     2161 cacggacacg caggcgacca ccgcgacgct aacaatgagc gcgcacggca agagaacacg
     2221 cacgagtgga ttgaaacgca tgagctaact ccttggcttc gcgcatacgc acgacgccca
     2281 gccttacaag accacccgat tgcgtgtgca tgatggccct aaggctccgt tcacggtcat
     2341 gagcggagtg cgcgccctgt cgtaggaatc cgccgcgctg gccagcggtt tggacattag
     2401 aatccgcaac gatgctacgg accatcctcg tatatggcgt attgctcgcg gccggcgcgt
     2461 tcggcctgca atggctggaa taccacttcg tggtgcgcac gcatgccacc gaaacctacc
     2521 tggtactcgt cgcgctggcg ttcatgggct tggggatctg ggtgggggcg cagctcttcc
     2581 ggcgtcggcc atccgctccc ttcgaaccga acctgaaagt acgtgagacg ctcggcgtca
     2641 gcgatcgcga attcgaggtg ctcacgctgc tggcggcggg tcgctcgaac aaggaaatcg
     2701 cgagccagct caatgtctca cccaacacca tcaagacgca cgtcgcaaag ctattcgaaa
     2761 agctcgaggc caggcgacgc acggaggcca tctcgcgggc gcgtgagctc ggaatgatcc
     2821 gttaactgcc ggattccggg cgatcagggc gaaatcaccc tttcatacga ttgccggccg
     2881 ccggcgtatg ccctctgatg cgctcacctt cactgacagg tgccccatga ccgcaatcat
     2941 ccttcgatac ggcgtcatcg ccggactcat catcggcgtt cccatgttgt ggcgcatgct
     3001 ctccgcgaag cccggcgagg tgccggtcgt ggggatgctg ctcacctacc tcgtgatgat
     3061 cgtggcgctc agcgccgtgt tcatcggggt caaggcctat cgcgacaagg tgctcggggg
     3121 ggtcgtccgg ttcctgcccg cgctcggtgt cgggctcggc atcagcgcag tcgccagcat
     3181 tgtctatgcc ctcgcctggg aaatttcgag cgcgtacagc agtttcgact tcgtcgcctt
     3241 ttacaaggac tacatggtgg acgccgccaa gggcggctcg ccggccgaag tgaatcaggc
     3301 gatcgcgaac gccgaagcgt tcgagaagat gtaccgcaat ccgctgtatc gcatacccat
     3361 ggtattcgtc gagatgttcc cggtgggcgt gctgatttcg ctgatctcgg ccgccgtgct
     3421 caggaatccg cgcgtactgc cagcacgggg ggcagccccc atttcctagc agggaaaagg
     3481 ggacagatct attttccgcg aatcgagggg cgggcgagct cgacaacagg ggacgcggaa
     3541 aatagatctg tccccttttc cgcgatgcgg aaacgcccga cggcgccgcc aagctcaccg
     3601 gccagcatct gcaattcgtg cgcgctctcg cgcgcccgcg cgagcaggct gaaattctcg
     3661 ctgttgctgg tctcgacctc ggcgaccttg cgcgccacgg tttcgatgcc cacgcactgc
     3721 tcctgagcgt cgcgcgcgat ttcgccgacc acgtcggcca tggtcgccac gcgctcgacc
     3781 acgtcttcga tggtcttgcc cgcgctcgtt accagtgccg ttccctggcg cacggtcagg
     3841 ttgctcgctt cgatgagttc cttgatctcg cgcgccgagg ccgtgcagcg ctgcgagagg
     3901 ctgcggactt ccgcggcgac cacggcaaag ccgcggccgt gctcgccggc gtgcgccgct
     3961 tcgaccgcgg cgttcaacga gagcagattg gtgctgaagg cgatctcgtc gatgactgaa
     4021 accacgtcga cgacgcgcgc gctggtcgcg gacacttcgg ccatcgactt gacgacgttg
     4081 ccgaccacga ggccaccgcg taccgcggcg tcgcgcgctt cggtggcgat cgtgcgcacg
     4141 cgcagtgcgt tgtcggcatt gcgccgcacg gcatcggtga tttccttcat gttctgcacc
     4201 gccgcggcga tcgagtgcgt cagctcctcg ccctgcgtgt gcagcgtctc gttaccttcg
     4261 ttgatcgttg cggcgactga cgagacgttc tcggaaccgg tgcggatctg cgacaccatc
     4321 cgggagagat gcgagcgcga ggcggcgaga tcgtcgaaca cctgcgccag caatccctgg
     4381 tagcgatgcc cggccgtctc ggtcagttcg ccctggctga gcgcggccag ttgcagtgag
     4441 gtggcgtgca gcgccgtgtg aaacatttcg gtgagctggt tcagcgagct cgcgagctcg
     4501 ccccagtagc cggattttcc gtcgccggga atgcgctgtg agaaatcccc caggcccgcc
     4561 gcgtgcacca cggcgctcac ttcgcgctgt gcgcggactt cgtcggtgcg atccacccat
     4621 tcggcgacgc ggccgagcag ctcgcccttc tccgtcgtga tcgtcgatac aactacgtcc
     4681 gtcaccgtct gcagataacg caggcgcgtg gaccgcggat caccggaagc ggcgccttcg
     4741 ttggcgctgg ccgcagaaag caaatcgtcg agctgccggc cgacaatcgc ggtgccgggt
     4801 tgaatcaggt ccatcagcga gcgagcggcc gcgttcgcgt aaatgatgcg tccatcggga
     4861 tcggtgatga gcacgcgcga ggaggcgagg tccaggcccg aacgcacgcg gaaagcgccg
     4921 accgcggcgg acgccgccga ttcggccgcg gtgcgccgct cgatgagctg ctggcacaga
     4981 tcgctcatcg cgcgcaccag ctcgccacat tccgccgggt catcggattt gcgcacctcg
     5041 tcgtagtcgc ccgtcgccag gcggcgcgcg gtgagcgcgg cgtattgcac tggctcgatc
     5101 acggcattac gcaccgcccg gaatgcccgg aaggcggcga acgcggcgat cagcaccagc
     5161 accgcggcga cgccgagcgc gatccagaaa tcgatccggc cggccttgag cacgccggag
     5221 agtgaacccg cggaggtctg gttcagcgcc gtgacggtgg agatgacgta ggccagcaga
     5281 ttgggatcga aggtgacctg cagctcgcgc tgggcatcct cgacgcgccc gcgttgcgcc
     5341 aggcgcgttg ccttgacgcc gctgacgacg aaggtggaca agccgtcgct gacggcctgc
     5401 tgcagcgacg gatcgctcga cagcttggtg gagatctcgt ccaccacttc gccaaggcgc
     5461 gccttggctt cgttgaaagc ggcgatgccc ttgttgcgtt gttcgtcgtc ggtcgccgcg
     5521 acggtttcac gcgcgtggtc ggagagttcc tgcgccgcgt tcagcagacg cccggtgagc
     5581 tccatgctcg cgttgctgtt ggtactggag tcctcgagcc tgccgaccag cacccatgcc
     5641 gatccgctcg acagcgccgc ctggaccagc agcagaagga tcaggacgag cccgggaatg
     5701 gcgagccggc gtgaaaccga ttcgccgatc cagccgcgaa gtttcgcgat ggacttcacg
     5761 ggcgtcagac ttcagttggc cggcgtgccc gtcttgcgca gctcgcgcaa gaaggcaacg
     5821 agatcgtgaa tctgctcgtt cgtgagttcg tcgccccagg gcggcatgaa agccgaacgg
     5881 ccgacggaga caccaccgcc gcgaatgatc atttccttgt actgatcggg gaaagggctc
     5941 acggtcaggt tggccggttt cggcgtgtac attttcgcag cgcgcccatc acccttgccg
     6001 gcggcgccgt ggcacaacac gcaatacgtg cggaacgcga tgccgccgcg gatcaccgac
     6061 gcatccgccg tatcgcgcgg cagcgggccg gcgtcgtctt cggcccaggc cgtcggcgca
     6121 cccaacggag ctgcgagcat caacaacagc gcggcggaga ctgccaattt cacttcgcga
     6181 cctcgatctt cattttcatt tccggatgaa tggcacattc gacttcgatc gtgcccggcg
     6241 cggtcagctt cacattgcgg gtttcgccct tcttgtatga acccaggtcg aagctctgag
     6301 cgtcgctcag cgaaaagacg ttgtgcgcga attcatcgtc gttgtggaac gtgatggtgt
     6361 cgcccacctt cgccttgagc acgcccacgg tgaatgcctt cttggcctgt ccgaccgtct
     6421 ggtcaccggc gaatgccggc atcgcgcagg tcatcaccac agccaggagg ctcaagcgaa
     6481 ttgcagaaaa agaacgcgga gtcattgtca gatcctcttc agttattggg gcagagccgg
     6541 caggcggaca tccgcgggga cgcccgtgag cgactcgagg aaggcgacca ggtcggcttt
     6601 ctcgctggcc gtgagcgaca gcgggaccat gttgatatcg agattggtct tgtcatcgcc
     6661 gccgcggtcg tagtgctcga cgacttccat gagcgtcttg tacgaaccgt tgcgcatgta
     6721 cggcgccgtc agcccgatgt cacgcagcgt cggcgtcttg aacgcgccat gcatggactt
     6781 gagcggacgc tgcgcgaagc ggcccacgtc gggcgcgccg gaggttgcaa ggccgatgtt
     6841 gtgaaaaccg ttgtcggtga agttgtagcc catgtggcac accgcgcact tcgccttgtc
     6901 ctggaacagc tcgaagccgt gcttggccga ggcgctaaca gccttctggt cgcccttgcg
     6961 ccagcggtcg aacggtgact cggtggagag caccgtgcgc tcgaagctcg cgagtcctcg
     7021 cgccacggtc accgcggtga tgcccatgcc gggataggcg gcctcgaact tcgaggcgta
     7081 gcccgggatg gacttcaacc gctcgatgag ctcgggcagc ggcaggttca tttcaccggc
     7141 ggcttcgata gggccggtgg cctgatcttc gagcgtggac ttgcggccat cccacatctg
     7201 aatcggactg aaagaagtgt tcacgatggt cggggtggcg cgcgccagcg tgtgcatgtc
     7261 aaagcccacg gcggtgggca gtccatccga ccagcccagc gaggggttgt ggcagctcgc
     7321 gcagctcatc gtgctcttgc gcgacaggcg cggatcgaag aacaacaact gaccgagatg
     7381 cacgcgctcg ggcgtcagct ggttttcagg cggggccggt gcgaactccg ggcggagcca
     7441 cggatccgac tccgaggcga ccacgctgcc gatgagcagg aatccaaaca gcaaggtcag
     7501 cggcagcgaa actcgctggc tgatgaattt atgcgacatg cgggcacgac tccgatgcga
     7561 tccgattgat cgttgggggc tgaatcggcc gggaaatcgc tgccttaagc gcggctggat
     7621 cgagtcttca ggagtgtgag gtgcttcatc ctggcggagg aagggttccc ctcggccatg
     7681 gacataatca aaaactcagg gattccagcc gtcgctccct gcgagaaaat ttttcaaaga
     7741 ccacgccttc gtctcggatt tcgtgagctg cacagcgcga ggttcacgcg tcgcaatgct
     7801 cgcgccctgc cccgtggatc gaaactcccg atacgtcgcg gtcttgaatg tctcggtggt
     7861 gcccggcgtc cattcgcgcc agcctgcggg ctgcaacggc gcgtcgatgc gcgtgcgcat
     7921 gaacaccacg gtggcatagg ggcgccacgc gcggcccagc cagagttcct tcactccagc
     7981 gtcggcggtg atgcggcagc gatcgaacac ataggcgctg tcctgcccgg gcttcacctt
     8041 gctttgcgca gtgatcatca cgatgtcgtt ggcgatgcca tgaatgtggc agcgatcgaa
     8101 gaacgccttg gcatcgccga agatgaagtc gacgtggcct tccacgtaac aatcgcgaaa
     8161 gtactgacgg ctgggtgaat ccgggttctt gctggcggcg tacaaagtgt cctgcgcccc
     8221 taacaaccgt acgttcgtga gcacggcgcg atcggcggtg aggcgcagcg ccaccgcctg
     8281 ggagcgttcc gtattcccga ggtgatagtc gttctggatc gtgaggttgt gcgcctcgaa
     8341 tccgtcgccc gaagcccata cggacgcgga tttgcccgtg cctcccgcgg acttgttgct
     8401 gtcaccccag accagcacca cgttctccgg ccgattgccc ttgctcttgc cgatgagttt
     8461 cacgttgggt ttcgcgatct cgagcttttc gcgatacgtc ccggcgccga tcacgatcat
     8521 tccgccggat tccggcagcg cgtcgacggc cgcctgcacc gactgataat cattcgtctt
     8581 cggattgccg accgtgaggg tcgtggcgaa actcgtctgc gccaacagca ccgccagtcc
     8641 caacatcgct ggtttcatgc gccgggattc tacccaatcg ctatgcagca atcgaaacgg
     8701 tgagaggatc agcagcctca cgacattagc tagtaatcgg tagtcgctct ctcaaccttc
     8761 ctgaggtgcg tcatgtccca agccaaatcc cccgtcacga attcacgccg cgcgttcctg
     8821 cgaaatgtcg gcgtcggcac gagcgcgctc ggcgtcctgg gccccggcgc actgctcatc
     8881 ccatccgtat cgcaggcgat ctgcgagcct cccggcacgc cgggcagcgc caggccctgg
     8941 cgcgcggact gccgccccat ccgggcgcgc cgccccgctt cgacgctttc cgccgcggaa
     9001 atccagaagc tcaaggatgc ctacaaggcg atgcgcgacc tggcggtgag cgaccccagc
     9061 gatccgcgcg gcttcaccca ccaggcgaac atccactgct ggttctgcag cgtgagcccc
     9121 acaccggtcc acggaaactg gcagttcttc gcgtggcatc gcgcctatct gtattttcac
     9181 gaacgcatac tgggcaagct catcaacgac agtgagttcc ggctcccgta ctgggactgg
     9241 gaaatttcca cgcatcgcca gatgcccggc gcctacacca cgccgaacaa caactccaac
     9301 cccctgtgga acagcacgcg cgccatgtca ccgacggacg aactgcccga cgaagacgtg
     9361 aacgaggacg tgatggagcc ggtgtacagc gccgcgaact tcggcgagtt cggcggcggc
     9421 gcgggctact ccggaattcc cgagggctct ccgcacgggt ccgtgcacgt ggacgtgggc
     9481 ggattcgccg gcgacatggg tgcattcgat accgcgggcc gcgatccgat cttctatgcc
     9541 caccacgcca acgtcgacaa gatctggtcc gactggaaca agggcgcgtc gagccacgag
     9601 aaccccaccg accccgcatt cctcaatctc agctggtcgt tctatgacga gaaccggatc
     9661 tggcggtcca tcaaggcgtc gcagctgctg aatcacgaga cgcagcttcg ctacaactat
     9721 ggtttgtcgc gcttccggga aatcctgccc tgcattctcg actggatcat cgtgaggcca
     9781 ccgtggaaat tcgaccgccc ggtcatcgtc gagccgcgct tccgcgacca gatcacgaag
     9841 gctctcgacg ggcggcggcc cgtgcgcctg cacatcaaca gtccgcagat tcccctcgac
     9901 aagagcgccg tgtatcgcgt gtatgccaac ctggagtcgg ccgcagcgga tgaaggaccc
     9961 aagagccccg cgttcctcgg cgtcatcccc ttcgtgctca acaatcggga gaagcatcat
    10021 ccatcgaagg caccgcaagt cgtattgaac gtgagccggc gaatctcggc gctgctgcat
    10081 tccaccgacg gtattcagct cgcatacgtc gagcgtccgg cgaagagagc caagccttcc
    10141 ggcaaggcgc aactgctgac ggccagagag gtctacttct cttacggtga aacgatgcgt
    10201 gaataagttc tggtgggtgt cactgtccgt ggccctgggc ctggtcgcga tgtcagccct
    10261 gggcgcggaa ccggtcgaga gttcccccgg cagtacgaag gaattcgtgg tggcgatgcc
    10321 gcccgacatc ggctcggcac gcagcgtgat tctggtgttg agcgacgtgc gcctgccgcg
    10381 aatggccgcc gtcgtgctgc gtgccaggct cgtggaaacc aaagccgtga agggtggcgc
    10441 tgacatcgtc gcgctgttgc cgctgggctc cgtcggcctg ctggcggaat ctgcgaatgc
    10501 gcaaggcgtc acacaccact ccgtcgtacg catcgaggtc acgaagaagc tcaaacgctg
    10561 gcggcaggat catccgggcg tcagttcact gaacatccgc gtgattccat tcgcgggcgc
    10621 ggatccgctg ccgcatctcg aatggtcggc cgcctccgcg cggctgtcgc tgtctggtag
    10681 ttagggcggc cgtttgggct gcgcagcgcc tggaatcacc tccagcgttc catcgacctt
    10741 cgcgcgccag gttccatcgc gcctgatgag caggctttcg aagttggcgc gcccatcgtc
    10801 atcgcgccgc ggatacacgt tcaccgcgat gaccttgccg tcgtacagcg acctcaccgt
    10861 gggaacgatg gtgtgaccga tgagaatccg gtgtgcgccg aacttttcca acgcgaggtc
    10921 gacgtccgcc atcgtggccg tcggaaacgt cgtctgctct gcgaaatagc cgcggtacca
    10981 cagcggcccc gtgctgccca tgagcaaggc cgcgcgttcg cgttgcgcat cattcgtggg
    11041 catggagcca tcgagcagcg aacgcacgcc cgcattgatt tcgcgcaacg acaggccgct
    11101 gtcgatgagc gcgcgggaaa tgccggcgtg caggcacagc aggtcgttga tcttgagcat
    11161 cgtgggcttc gtgcgcagcc actggccgag cacgctggtc gcgccaaaca actccgaata
    11221 cgaaccgacg cccagcaatt gcgccgtctg cacgtacttg ggattcagat atcgcaggtc
    11281 cccgagcaac accatggttt cgtggttgcc gagcaccagg tgcacgccgc cgcccgcgcg
    11341 ccgtgcctcg gcctctagct ggtagagaag ccagagaatc tcggtgtggt ttgggccgcg
    11401 gtcgaagaca tcgcccagca ctaccaggtc tccacgccca aacgtccatc cgagctgcgc
    11461 gttgacgacg ccatgcttct ggagcatggc caccaggatt tcatattcgc cgtgtgtgtc
    11521 ggcgacgacg aacagcggcg ccttgccgga ggttcggatc tcatccgggg ccggcgttgc
    11581 cgggccgcga agcttgacct ggaacgcggg caggttaccc acggctttca cggtgatcga
    11641 cgcacccgcg gccagcggca gcgcgcgctt gcgcggaacg ccggcggcct cttcgaccgt
    11701 cacgccctgc cagccggcgc cgtcacccag cacgtagggg ccatcggccg cgccggcgcc
    11761 aacggtgaac agcaggatta cgaagaaaaa agcggcagct cgcatcgacg caggataccg
    11821 tacacggcat aacaagaatc ccagggagtt cacgatgtct cgttttctca ttgcgctcat
    11881 gctgcttccg gtcgtcggct acgcgcaggc gccagccgtc gaatcagcga aagccgtgct
    11941 ggtcacgggc gccagcaccg gcatcggccg caagatcacc gaacatctgg caggcaaggg
    12001 atatttcgtc tacgccggcg cgcgcaagca ggccgacctc gacgctctca acgccatcag
    12061 gaatgtgcag gcggtaaagc tcgacgtcac ccggcaggac gacatcgacg cagccgttgc
    12121 cagcatcacg aaggccggtc gtggactcta cgggctcgtc aacaacgccg gtgtagcgac
    12181 cgtcgccagc gccaccgaaa ccagtgtcga ggaattcacg ctgaccatgg aagtgaactt
    12241 gtttggtccc tggagggtga cccgggcgtt cgccccgctg atcatcgcta acaagggccg
    12301 catcaccacc attggttcga tctccggcat tctcgcgcca cgcgacctga ccgcgtacgc
    12361 catgagcaaa cacgccatgg aggcgtttgc ggattccgtg gccgtgcaga tggcgccgct
    12421 cggtgtgcag gtgagcatcg tcgagcccgg caacttcgat tcggacatcg gcaagagcgc
    12481 caccaaacgc accggcaagg aatcgaggtt cacggatcgc agcaagtaca agaagcccga
    12541 cgaagtcgcc gcggccgtgg aacaggcctt gttcgaagcc acgccgaagc gccgttacat
    12601 ggtgacccca gaccagcggg aagcggaaat cacgatccgc aagcagatcg aacagctcgt
    12661 gcagctcaac gaagggcatc cgtacacgta tgaccgcgat gcgctgatca ggatgctgga
    12721 cgagtcactc gcgaccgcgc ggccgaaaac gaagtagcgg gaaaaattac ttcgcgcagc
    12781 agcgcgccag cgcctcgcgc aactcccgca gacgtggagg cttggcgagc accaggtcca
    12841 cgtgcgcggg cagatcgcct tcggcctgca gccgctgtcc ccagccggtg agcaggatca
    12901 ctggcgaggt cgacgagatg gtcttcaccg cgttcgccac ctggcgtccg tccacatagg
    12961 gcatgcccag gtcggtgacg accgcggaga aaacctgctg cgcttcgtgc gcctcgcgga
    13021 aggtctcgat gccttcgcgc ccgccgttgg ccgtgacgat ggtatggccg tcggcctcga
    13081 gcgcatcacg cagcgaat
//
LOCUS       DASQJS010000335         9077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3354298, whole genome shotgun sequence.
ACCESSION   DASQJS010000335 DASQJS010000000
VERSION     DASQJS010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3354298"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VEW08_13430"
     CDS             complement(<1..769)
                     /locus_tag="VEW08_13430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative urea ABC transporter substrate-binding
                     protein"
                     /protein_id="HYJ41788.1"
                     /translation="MPIRSSTVSVRRLLQTLFIAVLAGAALLTTQAARAAEKTTFNVA
                     WSIYVGWMPWDYAQSSGILKKWADKYGITIKLTQMDYIESINQYTAGKFDGCVMTNMD
                     MLTIPSAGGVDSTALIVGDYSNGNDGVVLKGAGKKLADIKGQSVNLVELSVSHYLLAR
                     GLETVGLKERDIKVVNTSDADIVGAFAAKDSTAVVTWKPQLAEITKMPNTSLVFDSSK
                     IPGEILDLMVVNSATLKANPNLGKALVGAWYETLKVMF"
     regulatory      complement(801..910)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            1114..1818
                     /locus_tag="VEW08_13435"
     CDS             1114..1818
                     /locus_tag="VEW08_13435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP1"
                     /protein_id="HYJ41789.1"
                     /translation="MLWEEEIPGGAHWSGVLRRGVVLRLIAGGDDANVAALFYSREQP
                     LERYNMADTLKAQHIAHLTTGCVCYSDMGRILCSITADSCGWHDPLCGLTDAAHITSR
                     YGATRFQEQRNQRYVSGRDAMLVELGKYGLNERDLVANINFFSKVAVDEAGALKLAAG
                     HSRAGQYVDLRFEMDTIVALHAGPHPLATATRYAPPAIRLQARRGTPPAADDPCRVRC
                     PENARGYINTERMYLS"
     gene            1815..2432
                     /locus_tag="VEW08_13440"
     CDS             1815..2432
                     /locus_tag="VEW08_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005404253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP2"
                     /protein_id="HYJ41790.1"
                     /translation="MNDEVVPAGEPWMGVVRRGQSLRIVDLEGNQAVDTLFYNAADTA
                     EHYSAQNTIQAQGRIYLTTGTVLLSNRGNPLLRIEEDTCGRHDTLGGACSAESNTVRY
                     ALEKRSMHSCRDSFLLALAQHPQTSGGALTKRDLPSNINFFMNVPVTPAGELRFADGI
                     SAAGRHVQLRALIDTLVLISNCPQLNNPCNAYNPTPIRVMTWDNA"
     gene            2524..6045
                     /locus_tag="VEW08_13445"
     CDS             2524..6045
                     /locus_tag="VEW08_13445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HYJ41791.1"
                     /translation="MFPKILIANRGAIACRILRTVKALGLSSVAVYSEVDENSPHVRL
                     ADEAFCIGPAPAADSYLRGERILEVARQSGAQAIHPGYGFLSENADFAESCERAGYAF
                     IGPTPSQMRDFGLKHTARALANECGLPLLPGTELLADLPTALAAADGIGYPVMLKSTA
                     GGGGIGMRRCESAAELSESFAAVQRLAAGNFRNSGVFLEKFVARARHIEVQIFGDGQG
                     AVIALGERDCSVQRRNQKVIEESPAPGLDDSTRQELHAAAVRLGEKVHYRSAGTVEFV
                     YDAAAAKFYFLEVNTRLQVEHGVTEEVGGIDLVEWMIRTAAGAPPRLREHRHVAAGHA
                     VQARIYAEDPARGFRPSSGLLTQVKLPGELRVDSWVDAGTEVTGYYDPMLAKVIARGA
                     TRELAIASLTAALRQSRIDGIETNLPYLVSILEHPVFATGEQTTAMLQGHAPPTSSIE
                     VLAAGTLTTVQDWPGRLGLWDVGVPPSGPMDALALRLANRMVGNEEGAAALEMTVTGA
                     TLRFEADAVIALAGAQMEATLDGVPAKFWYPLPVKRGSVLALGKIAGAGQRAYLAVRG
                     SFDIPEYLGSRATFTLGQFGGHGGRALRAGDVLRLNRNGLALDGCRAIDAHERPRYGS
                     EWQIGVHDGPHGAPDFFTAEDMRTFFDAEWEVHYNSSRTGVRLIGPKPTWARSDGGEA
                     GLHPSNIHDNAYAIGSVDFTGDMPVILGPDGPSLGGFVCPVTVVRDELWKIGQLRPGD
                     RVRFQRIPQLAPRADNPSVLLELPASQKHAAICVRRAGESNLLVEFGAPVLDLVLRFQ
                     VQALLETLRAQPLAGLLDLTPGIRSLQIHFDAVRATQAQVIDWLLAAIEKLPSVDRMS
                     LPSRIVHLPLSWDDPATQLATERYKAVRADAPWCPDNIEFIRRINGLESREAVRDIVF
                     GASYLVMGLGDVYLGAPVATPLDPRHRLVTTKYNPARTWTPENAVGIGGAYLCVYGME
                     GPGGYQFVGRTVQMWNRYRQTAEFRDGKQWLLRFFDQIRFYPVSSDELMKMRAGFPYG
                     RHPLRIEESRLDLGGYLRFLEEQRESIDAFRTRQRFAFDAERERWRAAGLAETPVESA
                     CRIEAPALPEDCIAITSPVSGSVWQIKVAAGQAVAKGETLVIMEAMKMEIAVIADSAG
                     TVVSIHGEPGTGARAGDTLLLLRPELS"
     gene            6042..7838
                     /gene="atzF"
                     /locus_tag="VEW08_13450"
     CDS             6042..7838
                     /gene="atzF"
                     /locus_tag="VEW08_13450"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HYJ41792.1"
                     /translation="MTLDLTISSLTRAYRAGTLTPSKLVERLIAERAKHAASNIWISS
                     VDDASLRYRARQLDVADQTSLPLYGIPFAIKDNIDFAGLPTTAGCPAFAYQPASSAPV
                     VQALIDAGAIALGKTNLDQFATGLVGTRSPYGACKNAFDPAYISGGSSSGSAVAVALG
                     LASFSLGTDTAGSGRVPAAFNNLVGYKPTLGSLSMRGVVPACRSLDAMSIFALHAEDA
                     AHVARVAVKYDAADPWSRTVGRPARAGFGRASTFRFAVPRAGQLDFFGNAAYASLFDA
                     AIRHLCALGGEPCEVDIEPLLSTARLLYEGPWVAERYLATESLLASQPDAMWEVTRQI
                     ISGGANAKALDAFRALYRLKELTRQAQAVWDAAPVLLLPTAGTHYRIAEEQADPIRLN
                     STLGRYTNFVNLMDLAAVALPAGFTPAGLPFGVSLIAPAWSDTDLLALAARAHRTMAA
                     TLGATSLPLPPAESLPAADGAIDVMVCGAHMSGLPLNKQLQERGAWQISTTRTAPVYR
                     FYALPGGPPFRPGLVRVRENGVAIDVEVWRVPAEHFGSFVAGIPAPLGIGKVKLEDGS
                     EVSGFMCESLGVADATDISALGGWRQYLASRS"
     gene            7986..>9077
                     /locus_tag="VEW08_13455"
     CDS             7986..>9077
                     /locus_tag="VEW08_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41793.1"
                     /translation="MHNFHHKLLACALALMLSACVGRSDPSKAVEPPLTLGDVDAAAD
                     QVAENAPIGSTVGVTVAVQAPGSGTLSYSLTDDAGGAFSIDATTGVVRRSGAVDFEAT
                     TGRSITAHAVLVDGTRRNVGARTFIIAVLDSPTPALELTFPFVHARHSDSIIAASGRV
                     THPQLQTVQITARAGGATVTGTITGERFTVRDIPVSGGAEYTLTVTASHAGGDSVSRQ
                     MTLSREPELTDVQQMVLDPTRNRVLLADRDSGSIIAAPLDGSARVLVSGRHVGTGPAF
                     MRPAAITISPQGDSFFVADGELDAIFSVSMATGDRSRLAPGNPRIFDPNELDFDTVRG
                     KLLLSDEQAGVLSIDVATGARQVVSSSQSP"
BASE COUNT         1560 a         3054 c         2883 g         1580 t
ORIGIN      
        1 cgaacatcac cttcagcgtc tcgtaccacg cgcccaccag cgccttgccg agattcgggt
       61 tcgctttcag cgtggcgctg ttcaccacca tgaggtcgag aatctcgccg ggaatcttgc
      121 tggagtcgaa cacgagcgac gtgttcggca tcttcgtgat ctcagcgagc tgcggcttcc
      181 aggtgacgac tgccgtggag tcttttgcgg cgaaggcgcc gacgatatcc gcgtccgagg
      241 tgttcacgac cttgatgtcg cgttccttca ggccgacggt ttcgaggccg cgcgcgagca
      301 ggtagtgcga caccgacagc tcgaccaggt tgaccgactg gcccttgatg tccgccagct
      361 tcttgccggc gcccttgagc acgacgccgt cgttgccgtt ggaatagtcg ccgacgatca
      421 gcgccgtaga gtccacgccg cccgcggacg gaatcgtcag catgtccatg ttagtcatga
      481 cacagccgtc gaacttgccc gccgtgtatt gattgatgga ctcgatgtag tccatctgag
      541 tgagcttgat ggtgatgccg tacttgtcgg cccacttctt gaggatgccg ctcgattgcg
      601 cgtagtccca gggcatccag ccgacgtaga tgctccaggc gacattgaac gtggtttttt
      661 cggctgcgcg ggcggcctgg gtggtcagca gcgcggcgcc ggcgaggaca gcaatgaata
      721 acgtctgcag cagacggcgt acggataccg tcgacgatcg gatgggcatg agagtctccc
      781 gtgttgttag tgcatcaggc atctcccggg cttttgtccc gccgtgtatc cccggcgcat
      841 cctcgcacca ctggactgcg tctctcggac cagcgctgat ttctcagccg gaaccctaga
      901 cgcacattct ggagttggtt tttgcaagag ctgtgccgtg agcacgcgtg gcgcgggccg
      961 acttgttagg gtcatcgcgg ggcatccatt gcaccaaaca aacgcgcgcg catcgatgca
     1021 cgcacactcg cggagcaatc cgcgtgcgta aagcctccag gaacccgcgg cttgagttca
     1081 tcgcgtggta cagcccttgc accggggctg tgcatgttgt gggaagaaga aatacccggc
     1141 ggggctcact ggtcaggcgt cttgcggcgc ggcgtggttt tgcggctcat cgcgggcggc
     1201 gatgacgcca acgtcgcggc gctgttctac agccgcgagc agccgctcga gcgctacaac
     1261 atggccgaca cgctgaaggc gcagcacatt gcccacctga ccaccgggtg cgtttgttat
     1321 tcggacatgg gccggatcct ctgttccatc accgcggaca gttgcggctg gcacgatccg
     1381 ttatgcgggc tcaccgacgc cgcacatatc acctcccgtt acggagcaac gcggttccag
     1441 gagcaacgca atcagcgcta cgtgagcggt cgcgacgcga tgctggtgga gctcggcaag
     1501 tacgggctca acgagcgcga cctcgtcgca aacatcaact ttttcagcaa ggtggcggtc
     1561 gacgaggcgg gtgcactcaa actcgccgcg ggccattccc gggctggcca gtacgtcgac
     1621 ctgcgcttcg agatggacac gatcgttgcc ctgcatgccg ggccgcatcc gctcgccacc
     1681 gcgacccgat acgcgccgcc agccatcagg ctgcaagcac gtcgcggcac tccaccggcg
     1741 gcggacgacc cgtgccgggt tcgctgcccg gagaacgcgc gcggctacat caacactgaa
     1801 aggatgtatc tctcttgaac gacgaggtgg tgcccgcggg tgaaccgtgg atgggtgtcg
     1861 tgcgccgtgg ccagtcgctt cgcatcgtgg acctcgaggg caaccaggcg gtcgatacgt
     1921 tgttctacaa cgccgccgac acggctgaac actacagcgc gcagaacacc atccaggcgc
     1981 agggccgcat ctacctcacc accggcacgg tgctgctctc gaatcgcggc aaccccctgt
     2041 tgcgcatcga ggaagacacc tgcggccgcc atgacacgct gggcggcgct tgttcggccg
     2101 agagcaacac cgtgcgttat gcgctcgaga agcgttccat gcacagctgc cgcgacagtt
     2161 ttctgctggc gctggcccag catcctcaga ccagcggtgg cgcgctgacg aagcgcgacc
     2221 tgccatcgaa catcaatttc ttcatgaacg tgcccgtgac ccccgcgggc gaactgcgct
     2281 tcgccgacgg catctccgcg gccggaaggc atgtccagct ccgggcactc atcgatacgc
     2341 tggtgctcat ttcgaattgc ccgcaactca acaacccctg caacgcctac aatccgaccc
     2401 cgattcgtgt catgacctgg gataacgcat gaacgcgttt tcccaccggc gggacgaccc
     2461 gccgcgcatt tcccccaacg ccgggacgac ccagcgtaga aaaaaaatgg gtggaactgc
     2521 gcaatgttcc cgaaaatcct gatcgccaat cgcggcgcca tcgcgtgccg catcctgcga
     2581 accgtcaaag cgctcggcct ctcctcggtc gcggtgtact ccgaagtcga cgagaactct
     2641 ccgcacgtcc ggctggccga cgaggcgttc tgcatcggcc ccgcgccggc cgccgacagt
     2701 tacctgcgcg gcgagcgcat tctcgaagtc gcgcgccagt cgggtgcgca ggcgattcac
     2761 cccggctacg gatttctctc cgagaacgcg gacttcgccg agagctgcga gcgtgcgggc
     2821 tacgcgttca tcgggccaac gccctcgcag atgcgcgact tcggcctcaa gcacacggca
     2881 cgtgcgttgg cgaatgaatg tggcctgccg ctgctgccgg gcaccgaact acttgccgat
     2941 ctgcccacgg cgctcgcggc agccgacggc atcggctacc ccgtgatgct caagagcacc
     3001 gccggcggcg gtggcatcgg catgcgccgc tgcgagtccg ccgccgagct cagcgaatcc
     3061 ttcgccgccg tgcagcgcct ggcagccggc aatttccgca actccggcgt gttcctcgaa
     3121 aaattcgtgg cgcgcgcgcg gcacatcgaa gtgcagatct tcggtgacgg ccagggcgcc
     3181 gtgattgcgc tgggggaacg cgattgttcg gttcaacgcc ggaaccagaa agtcatcgag
     3241 gagtctccgg cgcccgggct cgatgattcc acccgccagg aactgcacgc cgcggcggtg
     3301 cggctcggcg agaaagttca ctatcgatcc gccggcaccg tggagttcgt ctatgacgcc
     3361 gccgccgcga agttctactt ccttgaagtc aatactcgcc tgcaggtgga acacggcgtc
     3421 accgaggaag tgggcggcat cgatctcgtc gagtggatga tccgcaccgc ggccggtgcg
     3481 ccgccgagac tgcgtgaaca ccggcacgtc gcagccggac acgcggtcca ggcgcgcatc
     3541 tacgcggagg atccggcgcg cggcttccgg ccgtcgagcg gattgctcac gcaggtcaag
     3601 ctaccgggtg aactgcgagt ggactcctgg gtagatgccg gaacagaagt caccggctac
     3661 tacgatccca tgctcgccaa ggtgatcgcg cggggcgcca cgcgtgagct cgcgatcgcc
     3721 agtctcaccg cggcacttcg ccaatcccgc atcgacggca ttgagaccaa cctcccctac
     3781 ctggtgtcga ttctggaaca cccggtgttc gccactggcg agcagaccac cgcgatgttg
     3841 cagggccacg cgccgccgac ttccagcatc gaagtgctgg ccgccggcac actaactacc
     3901 gtgcaggact ggcccggccg cctggggctg tgggacgtcg gcgtgccgcc atcgggaccc
     3961 atggacgcgc tcgcgctgcg cctcgccaac cgcatggtgg gtaacgaaga aggtgcggcg
     4021 gcgctggaaa tgaccgtaac cggcgcgaca ctgcgcttcg aagccgatgc ggtgatcgca
     4081 ttggccggcg cccagatgga agccacgctc gacggcgttc ccgcgaagtt ctggtatccg
     4141 ttgccagtga aacgcggcag cgtgctcgcg ctgggaaaaa tcgccggtgc cggccagcgc
     4201 gcctatctcg cggtgcgcgg gagcttcgac atccctgagt atctcggcag ccgcgccacc
     4261 ttcacgctcg gtcagttcgg cggccacggc ggccgcgcgc tgcgcgccgg tgacgtgctg
     4321 cgactgaacc ggaacggcct cgcgctcgac ggctgccgcg ccatcgacgc gcacgagcgc
     4381 cccaggtatg ggtccgaatg gcagatcgga gtccacgacg gaccgcacgg cgcgccggac
     4441 ttcttcacgg ccgaagacat gcgcaccttc ttcgatgcgg aatgggaagt gcactacaac
     4501 tcgagccgca ccggcgtgcg tctcatcggc cccaagccca cctgggcgcg cagcgacggc
     4561 ggtgaagcgg gactgcatcc gtccaacatt catgacaacg cctacgccat cggaagtgtc
     4621 gacttcacgg gcgacatgcc agtgatcctc gggccggacg gccccagcct cgggggattc
     4681 gtctgcccgg tcacggtggt gcgcgacgag ctatggaaaa tcggacagct gcgcccaggc
     4741 gaccgtgtgc gattccagag aattccgcag ctggctccgc gggccgacaa tccttccgtg
     4801 ctgctcgaac taccggcctc gcagaaacat gccgcgatct gcgtgcgtcg cgctggcgag
     4861 agcaacctgc tggtggaatt cggcgctccg gtgctcgacc tggtgttgcg tttccaggtg
     4921 caggcattgc tggaaacact gcgggcccag cctctcgccg ggttgctgga cctcacgccg
     4981 ggcatccgtt cgctgcagat ccatttcgat gcggtgcgag cgacacaggc gcaggtcatt
     5041 gactggctgc tcgcggcaat cgaaaagtta cccagcgtcg atcgcatgag cctgcccagc
     5101 cgcatcgtgc acctgcctct ctcctgggat gatccggcga cacaactggc gacggaacga
     5161 tacaaggccg tgcgtgccga tgcgccctgg tgcccggaca acatcgaatt catccggcgc
     5221 atcaacggac tcgaatcgcg ggaagccgtg cgcgacatcg tgttcggggc cagttatctc
     5281 gtgatgggcc tgggagatgt gtatctcggc gcgccggttg caacgccgct cgacccccga
     5341 caccgcctgg tcaccaccaa gtacaacccg gcacgcacct ggacgccgga gaacgccgtc
     5401 ggcatcggcg gcgcctatct atgcgtgtac ggcatggaag gacccggcgg ctaccagttc
     5461 gtcgggcgca ccgtgcagat gtggaatcgt taccggcaga ccgccgaatt ccgcgacggc
     5521 aaacaatggt tgctgaggtt cttcgaccag atccggttct atccggtgag cagcgacgag
     5581 ttgatgaaga tgcgcgccgg atttccctat ggccgtcatc cgctgcgcat cgaggaatcg
     5641 cggctcgacc tcggcggata tctcaggttt ctcgaagagc aacgcgagtc catcgatgca
     5701 ttccgcaccc ggcagcgatt cgcattcgat gcggagcgtg agcgctggcg cgccgccgga
     5761 cttgccgaga caccggtaga aagcgcgtgc cggattgaag cgcccgcgct gcccgaagat
     5821 tgcattgcca tcacctctcc ggtctcgggc agcgtgtggc agatcaaggt tgccgccgga
     5881 caggccgtcg ccaagggaga aacgctcgtg atcatggaag ccatgaagat ggaaatcgcc
     5941 gtcatcgccg atagcgccgg cactgtggtc tcgatacacg gcgagcccgg tacgggtgca
     6001 cgcgctggcg acacattgct gctgctgcgc ccggagctgt catgacactc gacctgacga
     6061 tttcatcgct gacgcgcgcg taccgtgcgg gcacgctgac acccagcaaa ctagtggagc
     6121 ggctgatcgc cgaacgcgcg aaacacgccg ccagcaacat ctggatcagc agcgtcgatg
     6181 acgccagcct gcgttaccgc gcgcggcagc tcgatgtggc agaccagaca tcgttgccgc
     6241 tgtatggcat tccgttcgcc atcaaggaca acatcgactt tgccggtctg cccaccaccg
     6301 cgggctgccc cgcgttcgcc tatcaacccg cctcctccgc gccagtggtg caggcgctga
     6361 tcgacgccgg tgccatcgcg ctcggcaaga ccaatctcga ccagttcgcc accgggctcg
     6421 tcggcactcg ttcgccctat ggcgcctgca agaatgcctt cgaccccgcc tacatcagcg
     6481 gcggttcgtc gagcggctcc gccgtggcgg tggcgctggg gctcgccagc ttttcacttg
     6541 gcaccgacac cgccggttcc ggccgagtgc cggcggcatt caacaacctg gtgggctaca
     6601 agccgacgct gggtagtttg agcatgcgcg gcgtcgtgcc ggcttgccgc tcgctggacg
     6661 ccatgtcgat tttcgcgctc catgcggaag acgcggcgca cgtagcccgc gtggcggtga
     6721 aatacgacgc ggccgatccc tggtcgcgga ccgtgggccg gccggcacgt gcggggttcg
     6781 ggcgcgcgtc cacgttccgg ttcgccgtgc cgcgcgcggg acaactcgat ttcttcggca
     6841 acgcggcgta cgcgagcttg ttcgacgccg ccatacggca tctttgcgct ctcggtggcg
     6901 agccgtgcga ggtggacatc gaaccgctgc tcagcactgc gcgcctgctc tatgaaggtc
     6961 cgtgggtggc ggaacgatac ctggccaccg agtcgctgct ggcttcgcag cccgacgcca
     7021 tgtgggaagt gacgcgacag atcatttccg gcggcgcgaa cgcgaaggcg ctcgacgcct
     7081 ttcgtgcgct ctaccggttg aaggagctca cgcgccaggc acaagcggtg tgggacgccg
     7141 cgccggttct gctgctgccc acggccggca ctcactaccg catcgccgaa gagcaggccg
     7201 atcccatccg gctcaacagc acgctcggcc gctacacgaa tttcgtgaac ctcatggatc
     7261 tcgctgccgt ggcgctgccc gccggcttca cgccggctgg actgcctttc ggtgtctcgt
     7321 tgatcgcgcc cgcatggagc gacacggacc tgctcgcgct cgcagcccgt gcgcatcgca
     7381 cgatggccgc gactctgggc gccacttcgt tgccgctgcc gccggctgaa tccctgccgg
     7441 ccgcggatgg cgccatcgac gtgatggtgt gcggcgcgca catgtcaggc ctgccgctga
     7501 acaagcagct gcaagaacgt ggcgcctggc agatttccac gacgcgcacc gcccctgtct
     7561 accgcttcta cgccctgccc ggcggtccgc cgttccggcc gggtctcgtg cgcgtaagag
     7621 aaaatggcgt cgccatcgat gtcgaagtgt ggcgcgtgcc cgccgagcat ttcggctcat
     7681 tcgtcgccgg cattcccgcg ccgctcggca tcggcaaggt gaagctcgag gacggcagcg
     7741 aggtcagcgg attcatgtgc gagtcgctgg gcgtggcgga tgccaccgac atcagcgcgc
     7801 tcggcggctg gcggcaatac ctcgcctcgc ggagctgacg agtacgctca tcgcaactcg
     7861 tcgccctgcg tgtcacaaga ttcagtctcg tgacatgccc gagtgcgctc ggggcgggct
     7921 gaaaattttc attcgcaggt ggatggtgat ctgcaatact tcacgccggc tggcacggag
     7981 cacgcgtgca taacttccac cacaaactac tcgcctgcgc gcttgcgctg atgctgtccg
     8041 catgcgttgg ccgcagcgac ccgtcaaagg cggtcgaacc ccctctgacc ctgggcgacg
     8101 tggacgcggc agcggaccag gtcgccgaga acgcgccgat cggttcgacc gtgggagtga
     8161 cggtcgcggt gcaggcaccc ggcagcggga cattgagcta ttcgctgacc gacgatgccg
     8221 gtggtgcatt ttcgatcgat gcgacaactg gcgtcgtacg tcgatccggc gccgtggatt
     8281 tcgaggcgac caccggacgg tcgatcaccg cgcacgccgt gctcgtcgac ggcacccgcc
     8341 gcaacgtcgg cgcgcgcaca ttcattatcg ccgtgctcga cagccccacc cctgccctcg
     8401 agctcacgtt ccctttcgtg cacgcgcgac acagcgattc catcatcgcc gccagcggcc
     8461 gggtcactca cccgcaactg cagaccgtgc agatcacggc acgcgccggc ggcgcgaccg
     8521 tcacgggaac gatcaccggc gagcgattca cggtccgcga tattccggtt tccggtggcg
     8581 cggaatacac gctgacggtc accgcgtctc acgccggcgg cgatagcgtg agccgccaga
     8641 tgaccctctc gcgcgaaccc gaactgaccg acgttcagca aatggtgctc gatcccacgc
     8701 gcaaccgcgt tctgctggcc gatcgcgata gcggctcgat catcgcggcg ccgctcgatg
     8761 gcagcgcccg cgtgctcgtt tccggccgac acgtgggcac aggacccgcg ttcatgcgac
     8821 cagcggcgat caccatcagc ccgcaaggcg attcgttttt cgtcgccgac ggcgagctcg
     8881 acgcgatctt cagcgtcagc atggcgaccg gcgatcgcag ccgccttgcc cctggcaatc
     8941 cgcggatctt cgatccgaac gagctggatt tcgacacggt gcgcggcaag ctgctgctga
     9001 gcgatgagca ggccggcgtg ctctcgatcg atgtcgcaac cggtgcacga caggtggtct
     9061 cgtcgagtca gagtccc
//
LOCUS       DASQJS010000336         6467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3034237, whole genome shotgun sequence.
ACCESSION   DASQJS010000336 DASQJS010000000
VERSION     DASQJS010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3034237"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VEW08_13460"
     CDS             <1..1265
                     /locus_tag="VEW08_13460"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HYJ41794.1"
                     /translation="PNSPPGVLLPPPKPRVSDLLTKQRGAKLGIPVVAGHRAVLTKPL
                     DHTRLPKLLHPGNEKAQSILAADMQKRAACFFATPCGRGCSVRANYQSTTVHLPPAMD
                     TGNLDIVTDAMVRTVTKRPDGSAEGVIYIDRKTGAEHHVKARVVVLAASALESVRILL
                     NSGVANSSGKVGKYIMDTVGAGFSGQVPLLENLPLHNEDGVDGLHLYSPWWLYKEQLA
                     GKLGFARGYHIEFGGGRTMPGMGAASGLEWLNGGNYGKKFKEDARRYYGSQMWFSGRG
                     EMIPNDDCYAELDPKVKDKFGIPVLRFHWKWSQHEIGQAAHMEKTFAELLDAMGGRYP
                     RKPQSGDKAIKQGGIIIHEVGGAMMGDDPTKSVTNSFGQCWDMKNLFITDGATLCSNA
                     DKNPTLTIMALAWRASDRMLDLLKRKEL"
     gene            1262..1900
                     /locus_tag="VEW08_13465"
     CDS             1262..1900
                     /locus_tag="VEW08_13465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HYJ41795.1"
                     /translation="MSLDRRTTIKWMFAAAATVPSLHAIGYAPQPQARDVAANQAGYG
                     TDPDLTKEWRPGGPWPLTLGVAARLTTAALCDLIIPADDVSPAASAVGVVDFIDEWIS
                     APYPQQRSDREIILPGLTWIEAEAQGRFGKAFAALAQAQKEAIANDVCSPAKAAASAA
                     TAARFFAKFRDLTAGGFYTTPVGMKDIGYVGNVALAKFDGPPLAVLKKVGLA"
     gene            1908..2726
                     /locus_tag="VEW08_13470"
     CDS             1908..2726
                     /locus_tag="VEW08_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41796.1"
                     /translation="MSEPGPMLRRLPLRRVISGAFTLPWEHRGSVFRATAAPLLALIA
                     CTLAWDLSSFYISGIGRVVLLLIFYLPTLIAMSWLAVTVHRLVLLEAPDAAMHFDLGF
                     IRRVSTFAAVLFGIWVLFSGLTLLITSGVLNIFNPSRYVETSVEPGPVPQPMDLPVPF
                     EWITSIARILAYWFVARVSLMLPAIAIDRKADLGAAWQASRRNGWRLAIVVGALPWCL
                     QALVNLLYRDGASTIEFALLVVLGTMLIIFEVTALSLSYWELTPPAPPPTDPPA"
     gene            complement(2686..3411)
                     /locus_tag="VEW08_13475"
     CDS             complement(2686..3411)
                     /locus_tag="VEW08_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HYJ41797.1"
                     /translation="MAGAETLGMFDSPDSIVNRLGSLQLTIARRWSRALAATLFPPRC
                     CLCGFPGASLDLDLCAFCHADFPWEPENLPGTVVALRYAPPADELIRDLKYRGIAPNA
                     RVLGVLLAQAVEERSAPLPRLLVPVPLHDVRLRERGFNQAAALARYAGRMLGVPCMPR
                     AMKRVRDTPSQTSMSMEERHRNVSGVFAVNGVRAWRRLFDAGHVAVVDDVMTTGSTLA
                     EMKCALLSAGVRQVDLWAVARVA"
     gene            3475..4545
                     /gene="bioB"
                     /locus_tag="VEW08_13480"
     CDS             3475..4545
                     /gene="bioB"
                     /locus_tag="VEW08_13480"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HYJ41798.1"
                     /translation="MTAIRHDWTVAEVEALFALPFADLMFRAQETHRANHQPNAVQMS
                     TLLSIKTGACPEDCAYCPQSVRYDTGVERETLVPLEEVRTRALAAKEAGATRFCMGAA
                     WRSPKKRDVEAVSAMIREVRALGMETCATLGMLTPAQAHELKDAGLDYYNHNVDTSPE
                     FYGEIITTRTYQDRLDTLAAVRDAGLNVCCGGIVGMGETVRDRASMLATLANLPQHPE
                     SVPINQLVRVPGTPLAGKEPVDPFDFVRTIAVARVVMPKAFVRLSAGREAMADELQAL
                     CFLAGANSIFYGEKLLTTGNPDVARDRALLTRLGIEPLVPPAVAAAAERAHEETAGHV
                     HGPGCSHAAPRPSSSCATTPRR"
     gene            4521..5654
                     /gene="bioF"
                     /locus_tag="VEW08_13485"
     CDS             4521..5654
                     /gene="bioF"
                     /locus_tag="VEW08_13485"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HYJ41799.1"
                     /translation="MRHDPKALKTALADLEQRHLRRTRRVELHAGVINFCSNDYLGLA
                     KHPAVVGAFQAAAAKYGVGSGASHLVTGHGPEHEALETALAAFTGREKALVFSTGYMA
                     NMGVIGALADQSASLVSDKLNHASLIDGCRVSGAQSQRYRHGDAAHAFELLSALDSEV
                     DTRLLISDGVFSMDGDLAPLPDLARAARQTGAWLIVDDAHGLGVVGATGRGTCEHFGL
                     NAGDVPVLVGTFGKAFGTFGAFVAGDADLIDFLIQKSRTYIYTTALPPALAAATRAAL
                     EVSQREPWRREKVLALAERVRRALPEVFPVSRPGTAIQPVILGESQRALAASKVLENK
                     GFLVTAIRPPTVPAGTARLRITLSAAHEEAQVDGLIAALRELL"
     gene            5651..>6467
                     /gene="bioC"
                     /locus_tag="VEW08_13490"
     CDS             5651..>6467
                     /gene="bioC"
                     /locus_tag="VEW08_13490"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HYJ41800.1"
                     /translation="MTTPDPTKIFSLDRRAVLQAFDRASASYDAAAALQERVRNELLE
                     RLAELKVTPASILDLGAGTGHASRALKRAHPSAIVVAADIAPGMLARAKAQSRWLRRF
                     ERVRADAYSLPFRDASFDLIYSSLMLQWCDDLDTAFGEIARVLKPGGLLLFSTFGPGT
                     LAELREAWGKSGDAGNHVNHFFDPHAVGSALMHAGLSEPVLDVDRIVMRYPDARALMR
                     ELKAIGAHNVTAGRPRGLTGRKRLAAMTQAYESLRGEGGLPATYEVIHAICWGG"
BASE COUNT         1117 a         2160 c         2080 g         1110 t
ORIGIN      
        1 ccccgaattc cccgcctggc gtgctgctgc cgccgccgaa gccgcgtgtc agcgacctgc
       61 tcacgaaaca gcgcggcgcg aaactgggca ttccggtggt tgcgggccat cgagccgtgc
      121 tcacgaagcc gctcgaccac acccggctgc ccaaactact gcatcccggc aatgagaagg
      181 cccagtcgat cctcgccgcc gacatgcaga aacgcgccgc ctgtttcttc gccacgccct
      241 gcggccgcgg ctgctcggtt cgggctaact accagtccac cacggtgcac ctgccgccgg
      301 cgatggacac cggcaacctc gatatcgtca ctgacgccat ggtgcgtacc gtgacgaagc
      361 gccctgacgg ctccgcggaa ggcgtcatct acatcgaccg caagaccggc gccgagcatc
      421 acgtgaaagc gcgcgtggtg gtgctcgccg ccagcgcgct cgagtccgtc cgcatcctgc
      481 tgaactccgg cgtcgcgaat tcgagcggca aggtcggcaa atacatcatg gacaccgtgg
      541 gtgcgggctt cagcggccag gtgccgctgc tcgagaacct gccgttgcac aacgaggacg
      601 gcgtcgacgg tctgcacctg tacagcccat ggtggctcta caaggagcag ctcgcgggca
      661 agctcggctt cgcgcgcggt tatcacatcg agttcggcgg cggccgcacc atgcccggca
      721 tgggcgcggc gtcaggtctc gaatggctca atggcggcaa ctacggcaag aagttcaagg
      781 aggacgcgcg ccgctactac ggctcgcaga tgtggttctc cggccgcggc gagatgatcc
      841 cgaacgacga ctgttacgcc gagctcgatc cgaaggtgaa agacaagttc ggcattcccg
      901 tgcttcgctt tcactggaag tggtcacaac acgagatcgg ccaggccgca cacatggaga
      961 agacctttgc cgaactactg gacgcgatgg gcggccggta tccgcggaag ccgcagagcg
     1021 gcgacaaggc catcaagcag ggcggcatca tcattcacga ggtgggcggc gccatgatgg
     1081 gcgacgaccc cacgaaatcc gtgaccaaca gcttcggcca gtgctgggac atgaagaacc
     1141 tgttcatcac cgacggcgcc acgctgtgtt ccaacgccga caagaacccc acgctcacca
     1201 tcatggcgct ggcgtggcgc gcttcggatc ggatgctgga cctcctgaag aggaaagaac
     1261 tatgagcctc gaccgccgca ccacgatcaa gtggatgttc gccgccgccg cgacggtgcc
     1321 ttcgctgcac gcgattggtt acgcgccgca gccgcaggcg cgcgacgtgg ctgcgaacca
     1381 ggccggctac ggcaccgatc ccgatctgac gaaagaatgg cggcctggcg ggccctggcc
     1441 gctcacgctt ggtgttgccg cccgactgac taccgcggcg ctgtgcgatc tcatcattcc
     1501 cgccgacgac gtctcgcccg ccgcgtccgc tgtcggcgtg gtcgacttca tcgacgagtg
     1561 gatttccgct ccttatccgc agcagcgctc ggaccgcgag atcatcctgc cgggtctcac
     1621 gtggatcgaa gccgaagcgc agggccgatt cggcaaggcc ttcgcggcgc tcgcccaggc
     1681 gcagaaagaa gccatcgcca acgatgtctg cagcccggcc aaggccgcgg ccagcgctgc
     1741 gactgccgcc aggttcttcg cgaagtttcg cgatctcacc gcgggcgggt tctacaccac
     1801 acccgtgggc atgaaggaca tcggctacgt gggcaacgtg gcgctcgcga aattcgacgg
     1861 cccgccgctg gcggtgctca agaaggtcgg gctggcctga tcgcggcatg agcgaaccgg
     1921 ggccgatgtt gcgcaggctc cccctgcgca gggttatctc gggcgcgttc acacttccct
     1981 gggaacaccg tggctcggtg ttccgagcca ccgccgctcc actcctggca ctcatcgcct
     2041 gcacgctcgc gtgggacctc tcgtccttct acatctcggg cattggccgt gtagtcctcc
     2101 ttctcatctt ctatctaccc acgctgatcg caatgagttg gctggctgtc acggttcatc
     2161 gtctcgtgct tctcgaagcg ccggacgcag cgatgcattt cgatctcggc ttcatccgtc
     2221 gggtgtcgac cttcgcggcc gtgctcttcg ggatctgggt tctgttctcg ggcttgacgc
     2281 tgctgatcac cagcggcgtg ctcaacattt tcaatccatc caggtacgtc gagacatccg
     2341 ttgaacccgg gccggttccg cagccgatgg atttacctgt gccattcgaa tggatcacca
     2401 gtattgccag aatcctcgcg tactggtttg tcgcccgcgt gagcctgatg cttccggcaa
     2461 ttgccatcga tcgaaaagcc gacctcggcg ccgcgtggca ggcgtcgcgg cgcaacggct
     2521 ggcgtcttgc gatcgtggtc ggcgcgcttc cctggtgttt gcaggccctg gtgaatctcc
     2581 tgtatcgcga cggggcatcc acgattgaat ttgcgctgct ggtagttctt ggaacgatgt
     2641 tgatcatctt cgaggtgacg gcgctgtcgc tctcgtactg ggaactcacg ccacccgcgc
     2701 caccgcccac agatccacct gcctgacacc ggccgagagc aacgcgcatt tcatttccgc
     2761 cagcgtgctg cccgtggtca tgacgtcgtc gaccacggcg acgtggccgg cgtcaaacaa
     2821 cctgcgccac gctcgtacgc cattcacggc gaaaacgccg ctcacgttgc gatgcctctc
     2881 ttccatgctc atggatgttt gcgatggcgt atcacgcacg cgcttcatgg cgcgaggcat
     2941 gcacggaacg ccgagcatcc gtcccgcgta acgcgcgaga gccgccgcct ggttgaagcc
     3001 gcgttcgcgc agacgtacgt cgtgcaatgg caccggcacc agcaggcgtg gcagcggcgc
     3061 gctgcgctcc tcaacggcct gcgccaatag aacgccgagc acgcgcgcgt tgggcgcgat
     3121 gccgcggtat ttgagatcgc gaatgagttc gtccgccgga ggggcgtagc gcagggctac
     3181 caccgtgccg ggcaggttct ctggttccca tggaaagtcc gcgtggcaga acgcgcacag
     3241 atcgaggtcg agcgaggcgc ccggaaatcc gcacaggcag cagcgcggtg gaaacagcgt
     3301 ggccgcgagc gccctcgacc agcggcgcgc gatcgtcaac tgcagggacc ccaggcggtt
     3361 gacaatggag tcggggctgt cgaacatgcc cagagtttcg gcgccggcca tccgtgcggc
     3421 cagtcgaaaa atgagtgtaa tcaacgagta aagccaagag atcaaggaac aggaatgacc
     3481 gcgatacgcc acgactggac tgttgccgaa gtcgaagctc tcttcgccct gccgttcgcc
     3541 gatctcatgt tccgcgcgca ggagacccat cgcgcgaacc accagccgaa cgccgtgcag
     3601 atgagcacgc tgctgtcgat caagaccggc gcctgccccg aagactgtgc ctattgcccg
     3661 caatcggttc gatatgacac gggcgtcgaa cgcgagacgc tcgttcctct cgaagaagtt
     3721 cgtacccgtg cgctcgcggc aaaagaagcc ggcgccacgc gcttctgcat gggcgccgcc
     3781 tggcgttcgc cgaagaagcg cgacgtagaa gccgtgtcgg cgatgatccg cgaggtgcgc
     3841 gcgcttggca tggagacctg cgcgacgctg ggcatgctca cgcccgcgca ggcgcacgag
     3901 ctcaaggacg ccggcctcga ctactacaac cacaacgtcg acacctcgcc cgagttctac
     3961 ggcgagatca tcaccacgcg tacttaccag gaccggctcg atacgctcgc ggcggttcgc
     4021 gatgccgggc tcaacgtgtg ctgcggcggc atcgtcggca tgggcgaaac cgtgcgcgac
     4081 cgcgcctcga tgctcgcgac gctcgcgaac ctgccccagc atcccgaaag cgtgccgatc
     4141 aaccaactgg tgcgcgtgcc gggtacaccg ctcgcgggca aagagccggt ggatcccttc
     4201 gacttcgtgc gcactattgc cgttgcgcgc gtcgtgatgc ccaaggcatt cgtgcgtctc
     4261 tcggccggcc gcgaagcgat ggccgatgag ctgcaggcgc tgtgtttcct ggccggcgcg
     4321 aactccattt tttacggcga aaaactgctg accacgggca atcctgatgt cgcgcgcgat
     4381 cgcgcgctgc tcacgcgtct cggaatcgaa ccgctggtgc cgccggcggt ggccgcagct
     4441 gcggagcgtg cgcacgagga aactgccgga catgttcacg gcccgggctg ctcacacgcg
     4501 gcgccgcgcc cgtcttcttc atgcgccacg accccaaggc gttaaagacg gcgctcgccg
     4561 acctcgagca gcgccatctg cgccgcacgc ggcgagttga gctgcatgcc ggcgtcatca
     4621 acttctgcag taacgactac ctcgggctcg cgaaacatcc ggccgtggtc ggggcgtttc
     4681 aggctgccgc ggcaaagtac ggggtgggca gcggtgcgtc gcatctggtc acgggacatg
     4741 gacccgagca cgaggcactc gagacggcgc tggcggcatt caccggccgc gaaaaggcac
     4801 tggtcttctc gaccggttac atggccaata tgggcgtcat tggagcactg gcggatcaaa
     4861 gtgcctcgct ggtctccgac aagctcaacc atgcatcact gatcgatggc tgccgggtgt
     4921 cgggcgcgca gtcccagcgc tatcgccacg gtgacgccgc ccacgcgttc gagctgctct
     4981 cggctctcga ttcagaggtc gatacccggc tgctgatcag cgacggtgtc ttcagcatgg
     5041 acggcgatct ggctccgctg cccgacctgg cgcgcgcggc gcgacagacg ggcgcgtggc
     5101 tgatcgtcga tgacgcacat ggactcggcg tcgtgggtgc gaccggccgc gggacctgcg
     5161 agcactttgg cctgaacgcg ggcgacgtgc ccgtgctggt cggcacgttc ggcaaggcct
     5221 tcggcacttt cggcgcgttc gtcgccggcg acgccgacct catcgacttc ctgatccaga
     5281 agtcgcgcac gtacatctac acgacagcgt tgccacccgc gctggcggcg gcaacgcgcg
     5341 cggcgctcga agtgagccag cgcgaaccat ggcgacgcga gaaggtgctc gcgctcgcgg
     5401 aaagagtgcg gcgggcgctg cccgaagttt tcccggtttc ccggcccggc accgcgatac
     5461 agccggtgat tctcggtgag tcacaacgcg cgctggcggc gagcaaagtg ctcgagaaca
     5521 agggtttcct ggtcaccgcg attcgtccgc ccacggtgcc ggcgggcacg gcgcggctgc
     5581 gcatcacgct gtccgcggca catgaagaag cgcaggtgga cgggctgatc gcggcacttc
     5641 gcgaactgtt atgaccacac cggatccgac gaagattttc tcgctcgatc gccgcgcggt
     5701 cttgcaggcg ttcgatcgcg ccagcgccag ttatgacgcc gcggccgcgc tgcaggaacg
     5761 tgtgcgcaac gagttgttgg agcgcctcgc ggaattgaag gtgacgcctg cgtcaatcct
     5821 cgatctcggc gccggcaccg ggcatgcgtc gcgcgcgctg aagcgcgcgc acccgagcgc
     5881 tattgtcgtt gccgccgaca tcgcaccggg catgctggca cgcgcaaagg cgcagtcgcg
     5941 ctggctgcgg cgtttcgaac gagtgcgcgc cgatgcctat tcgctgccgt ttcgcgacgc
     6001 ttcgttcgac ctcatctaca gcagcctgat gctgcagtgg tgcgacgacc tcgacaccgc
     6061 cttcggtgaa atcgcgcggg tgctgaagcc cggcgggttg ctgctgttca gcactttcgg
     6121 gccaggcacg ctcgccgaat tgcgcgaggc gtggggcaag agcggcgacg ccggcaatca
     6181 cgtgaatcat ttttttgatc cgcacgcggt gggaagcgcc ctcatgcacg cgggactctc
     6241 ggagcccgtg ctcgacgtcg atcggatcgt catgcgctac ccggacgcgc gcgcgctcat
     6301 gcgcgagctc aaggccatcg gcgcacacaa tgtcaccgcc ggccgcccgc gcggactcac
     6361 gggccgcaag cgcctcgcgg cgatgacgca ggcgtatgaa agcctgcgcg gtgaaggcgg
     6421 attgcccgcg acctacgagg tcattcacgc gatctgctgg ggaggcg
//
LOCUS       DASQJS010000337         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3035000, whole genome shotgun sequence.
ACCESSION   DASQJS010000337 DASQJS010000000
VERSION     DASQJS010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3035000"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1465
                     /locus_tag="VEW08_13495"
     CDS             188..1465
                     /locus_tag="VEW08_13495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYJ41801.1"
                     /translation="MKLLALGVLLGFLIALGGDVFAHRDDEPAKSQRAERSSESRSPL
                     SAAEAAQLAEVFDRVQREYVDTVSHPELVDDALRGLVGGLDPYSTYLDAEEYADLRVS
                     TAGTYAGIGIEVSTADRALRVVRPFRDSPAAAAGIRSGDMISAIDGLPVGADLDAAMA
                     RMRGPRGSTVKLAVMRTGQSLPLEFTVERAQVDVHSVAMVKLDDAYLYARITSFSDTT
                     AEDFASSIARLRRDMQSKPRGVILDLRNNPGGVLESAVEVADQLLETGVIVTADGRTP
                     AARFTMEATPGEVLPGVPVVILVNGSTASAAEILAGALQDHRRAVLLGRRTFGKGSVQ
                     TVMPLAQGRAIKLTTSRYFTPSGRSIQGHGIDPDHDFENIDGVPLDLEDPRVRQTLAS
                     QDAGVHAALELLRGRKPKPASGMTASAGSAPWK"
     gene            1473..2117
                     /locus_tag="VEW08_13500"
     CDS             1473..2117
                     /locus_tag="VEW08_13500"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HYJ41802.1"
                     /translation="MEMILALFHWFVDVILHLDSHLVELLRDYGLWVYAILFAIVFAE
                     TGFVVTPFLPGDSLLFAAGALAAVDSSGTLSAPLVSLTLIVAAVAGNTTNYHIGKWIG
                     PPAFTGKIRFLKVEYLRRTETFFVKHGSMTIVLSRFVPIIRTCAPFVAGVARMPYARF
                     QAYNVFGGVSWVMLLVWAGYFFGNIPIIKENFGVVTIGIIVLSLVPLAWVALRK"
     gene            2114..2575
                     /locus_tag="VEW08_13505"
     CDS             2114..2575
                     /locus_tag="VEW08_13505"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYJ41803.1"
                     /translation="MSGAYRSFAHRVDVGVPVERVWRAFTDSRMLERWTTAGATVTPR
                     EKGRMQVMFGEGMELDAHIDVLVTAQRLRLILLPTRGMPGTDAVIVEDFLFERVPPAG
                     TAVRLMSSGVPSKQAWVTYYLSKRRYWELALARLKVFLEKGLDQEQPKPRY"
     gene            2579..2956
                     /locus_tag="VEW08_13510"
     CDS             2579..2956
                     /locus_tag="VEW08_13510"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HYJ41804.1"
                     /translation="MVGFILRGIVAALGLWAATEVLSGLNIDSPGTLILAGLLLGVVN
                     AIVRPFALLLSLPALIFTLGLFLLVVNAAMLGLVALLLPGFHIAGFWTAVGGSIIVSI
                     VSWVGSWFIGPRGRVEVIRAKHD"
     gene            complement(2966..>3457)
                     /locus_tag="VEW08_13515"
     CDS             complement(2966..>3457)
                     /locus_tag="VEW08_13515"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYJ41805.1"
                     /translation="ASHFGMQLLILLAVFVWSATTVYIRNVHSRLDLLSFSAIQMLVG
                     GLMMTGAGLAMGEAGQWTWSKPGLIAMAYLTIASSCLAYTAFSWLAKNTTPAMVGTYS
                     YVNPAIAAILGWWFLDEQLGANQLTGMAIMFAGVALVNWPQKDVRRLDEDEQKDPSTA
                     GCG"
BASE COUNT          548 a         1139 c         1104 g          666 t
ORIGIN      
        1 acgcgcgtgc gcatgctcgg cgtcgactac ggccagggtt tcgcgattgc ccgccccgtg
       61 cccgtgcagg aagtgctcga cgtgctgccg gtgctctgtg ccgccacacc cgggtcgttc
      121 ctgctcgacg agtcggccgc caacgccggc tgagctaact ccttaatttg agttaggatt
      181 cacgcccatg aagctcctgg cgctaggcgt gttgctcggc tttttgatcg ccctcggtgg
      241 cgacgtcttc gcgcatcgcg acgacgagcc ggcaaagtca cagcgcgcgg aacgcagctc
      301 cgaatccagg tcgccgctgt cggccgcgga agccgcgcaa ctcgcggaag ttttcgatcg
      361 cgtgcagcgc gaatacgtcg acaccgtttc ccaccccgag ctagtcgacg atgcgctgcg
      421 tggcctggtg ggcggtctcg acccctattc cacttacctc gacgccgagg aatacgccga
      481 cctgcgcgtg agcacggccg gaacctacgc gggcatcggc atcgaggtat cgaccgccga
      541 tcgtgcgcta cgcgtcgtgc gtccgtttcg tgattcgccg gccgcggctg ccggaatccg
      601 tagcggcgac atgatttccg ccatcgacgg attgccggtg ggcgccgatc tcgacgctgc
      661 catggcacgc atgcgtggtc cgcgcggctc caccgtcaag ctcgcagtga tgcgcaccgg
      721 ccagagcctg ccactcgaat tcaccgtcga gcgggcgcag gtcgacgtgc acagcgtggc
      781 catggtgaag ctcgatgacg cctatctata cgcgcgcatc acgagtttca gcgatacgac
      841 cgccgaagat tttgcctcca gcatcgcgcg gctgcgccgc gacatgcagt cgaagccgcg
      901 cggcgtgatt ctcgacctgc gcaacaaccc cggcggtgtg ctcgagtccg cggtggaagt
      961 tgccgaccag ctgctcgaga cgggtgtcat cgtcaccgcc gatggccgca cgcccgccgc
     1021 ccgcttcacc atggaagcga cacctggtga agtgctgccc ggcgtgccgg tggtcatcct
     1081 ggtgaacggt tccaccgcgt cggcggccga aattctcgcc ggcgcattgc aggatcatcg
     1141 ccgcgccgtg ttgctcggcc ggcgcacctt cggcaaaggt tccgtgcaga ccgtgatgcc
     1201 gctcgcgcaa ggccgcgcca tcaaactgac cacctcgcgt tacttcacgc catcgggccg
     1261 ctccatccag ggccacggca tcgatcctga ccacgacttc gagaacatcg acggtgttcc
     1321 gctcgatctc gaggacccgc gcgtgcgcca gacgctggcc agccaggatg ccggtgtgca
     1381 cgccgcgctc gaactgctga gagggcgcaa gcccaagccg gcgtccggca tgacggctag
     1441 cgcgggcagc gcgccgtgga aatgacacgc gcatggaaat gatcctggcg ctgttccact
     1501 ggttcgtcga tgtcatcctt catctcgact cccacctggt ggagctcctg cgcgactacg
     1561 gcttgtgggt gtacgccatc ctcttcgcca tcgtgttcgc cgagaccggt ttcgtcgtga
     1621 cgccgttcct gccaggggat tcgctgctgt tcgccgcggg cgcgctggcg gcggtggatt
     1681 ccagcgggac gctctccgcg ccgctggtct cgctgacgct catcgttgcc gccgtcgccg
     1741 gcaacaccac taactaccac atcggaaagt ggatagggcc gccggctttc acggggaaga
     1801 tccgtttcct caaggtggag tacctcaggc ggaccgagac atttttcgtg aagcacggct
     1861 cgatgaccat cgtgctgtcg cgcttcgtgc ccatcatccg cacctgcgcg ccgttcgtgg
     1921 ccggtgtcgc ccgcatgccg tacgcgcgct tccaggcgta taacgtgttc ggcggcgtta
     1981 gctgggtgat gctgctggtg tgggcgggct acttcttcgg caacattccg atcatcaagg
     2041 aaaatttcgg cgtggtgacc atcggcatca tcgtcctttc cctggtgccg ctggcgtggg
     2101 tggcgctcag gaaatgagtg gcgcgtaccg cagcttcgcc catcgcgtcg acgtcggcgt
     2161 accggtcgaa cgcgtgtggc gggctttcac cgacagccgc atgctcgagc gctggaccac
     2221 cgcgggtgca accgtgacgc cgcgggaaaa aggccggatg caggtcatgt tcggcgaagg
     2281 catggagctt gatgcgcaca tcgacgtgct cgtgaccgcg cagcgtttac gcctcatcct
     2341 gctgcctacc cggggcatgc ccggtaccga tgccgtcatc gtcgaggatt tcctgttcga
     2401 gcgcgtaccg ccggcgggca ccgcggtccg gctgatgagc agcggagtac cctcgaaaca
     2461 ggcctgggta acgtactatt tgtccaagcg ccgctactgg gagctggcgc tggcccgact
     2521 caaggtgttt ctcgagaagg gtctcgacca ggaacagccg aaaccgaggt actgactcat
     2581 ggtgggtttc attctgcgtg gcatcgtcgc cgcgctcggc ctctgggcag caaccgaagt
     2641 gctgagcggc ttgaacatcg attcgccggg cacgctgatc ctggcgggtc tgttgttagg
     2701 cgtggtgaac gcgatagtgc ggccctttgc gctgctgctg tcgctgccgg cgctcatatt
     2761 cacgctgggt ctgttcctgc tggtggtgaa cgcggcgatg ctggggctgg tggcgttgct
     2821 gctgccgggt ttccacattg ccggcttctg gactgcggtc ggcggctcca tcatcgtcag
     2881 catcgtgagc tgggtcgggt cgtggttcat cggcccccgc ggccgcgtcg aagtcatccg
     2941 cgccaagcac gactaggggc agctcctatc cacaccccgc tgtcgacgga tccttctgtt
     3001 cgtcctcatc gagtcgcctc acgtctttct gcggccagtt caccagcgcc acgcccgcga
     3061 acatgatcgc cattccggtg agctgattcg cgcctaactg ttcatcgagg aaccaccagc
     3121 cgagaatcgc ggcgatcgcc ggattcacgt agctgtacgt gccgaccatg gcgggtgtgg
     3181 tgttcttcgc gagccacgag aacgcggtgt aggcgaggca cgagctcgcg atggtgagat
     3241 acgccatcgc gatcagcccc ggcttcgacc atgtccattg gccggcttcg cccatcgcca
     3301 gacccgcccc ggtcatcatc agtccgccga ccagcatctg gatggcggag aacgacaaca
     3361 ggtcgagcct ggagtgaacg ttgcggatgt acaccgtggt cgccgaccac acgaacaccg
     3421 cgagcaggat cagtagctgc atcccgaaat ggctggc
//
LOCUS       DASQJS010000338         5669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2094053, whole genome shotgun sequence.
ACCESSION   DASQJS010000338 DASQJS010000000
VERSION     DASQJS010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2094053"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /gene="clpS"
                     /locus_tag="VEW08_13520"
     CDS             complement(<1..293)
                     /gene="clpS"
                     /locus_tag="VEW08_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HYJ41806.1"
                     /translation="MSETERPDQGGAGLAVEEAEPKTKQPPLYQVVLLNDDYTPMEFV
                     VDVLEKFFRMDRLTATRVMLEVHTQGKGICGVFTYEIAETKVALVTSYARDHH"
     gene            411..1373
                     /gene="gspC"
                     /locus_tag="VEW08_13525"
     CDS             411..1373
                     /gene="gspC"
                     /locus_tag="VEW08_13525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HYJ41807.1"
                     /translation="MEMTANFWNPGASSGGDWRSSLLARAPQVLTFVLALALAAQLAF
                     MVVAMTSRSRQAGPPPLAALPPPAQLDIGGLVNAHLFGNAAVQANGDAANAPPSSMPL
                     VLAGVLASEDPKEGMAIIGESAQAAKVVAVGQQVPGGAQLHSVYSDRAVIDRGGTLES
                     VFLPRQTGGGIVASAPPPMPTAAPEGNEAMIERMRKLVNDDPGLLSQVMRPQPVFAGG
                     EMKGFRVYPGANRQAFSRMGLRAGDMVTAINGTPLNDKDRAQEIFGTLGSSTDARVTI
                     TRNGRQQELVLNIAQITAEAERLGTGGDGNIPMEQAPEPVPGNE"
     gene            1436..3514
                     /gene="gspD"
                     /locus_tag="VEW08_13530"
     CDS             1436..3514
                     /gene="gspD"
                     /locus_tag="VEW08_13530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HYJ41808.1"
                     /translation="MTRKSFRVTLLAALFAFAATGIVSAQNNGQRITPSFKDAEIGQV
                     IEAVAAATGKTIIPDPRVRAQVTMLSQTPMTPDAFYEAFLALLSVHQFVAVESGGIIK
                     IVPDANARQMPNIDLPSRVKSGSDELITQVIAVHNVNAAQLVPVLRPLMPQAAHMAAY
                     PNGNILILSDRASNVSRVMRIIERIDQQGDNDVDIINLQHASSSEVVRVVNTFFAQQG
                     QAEGGGGSPSRVIADDRSNSVLVGGEKSLRLRIKALIAHLDTPLESGGDTQVRYLQFA
                     DAEKIAAKLKEQISATVAITTGAPPAGGGATSGVAASADRSTTIWAEPETNALIITAP
                     AKIMRSLMSVVDKLDIRRMQVLVEAIIVDINVNKNAELGVNWAVWSDDDDTRFPVGTF
                     QQPVGGVNLTSLAQSIDNPSGGNPLLGTGTTFGIGRIATSGVNFAAMLRAIQGDSNTN
                     VIATPSTVTMDNQEAELKVAQEVPFVTGSYSNTGNTGNNGEVNPFTTVQREEVGTILK
                     ITPQINAQGALVQMKIELESSSLTGDSGDANSLITNKRTISTNVLIEDGGIIVLGGLI
                     QDSDIRGEQRVPYLGRIPVLGALFKTRSRQTDKTNLMVFIRPKILYDGTQTAVETNAK
                     YNYIRDEQRAAGRPKSEVLPLLPYSKEPRLPPLAPPEVPMPPPLPTDSATGTTPSRPP
                     ANPEGPPPNP"
     gene            3511..4998
                     /gene="gspE"
                     /locus_tag="VEW08_13535"
     CDS             3511..4998
                     /gene="gspE"
                     /locus_tag="VEW08_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HYJ41809.1"
                     /translation="MNEVAEPGVDRPKRVGFAFAKRHGILVDRIHEGVAECVYRRGTA
                     PLAVAEVRRYLGIPIRLERVEDEKFDSLLRIAFEAGNDAMQAAEGVEESTDLAHLAQD
                     LPEQADLLESEDDAPIIRLINAVLTQAVKENASDIHVEPFENRLVVRFRVDGVLREVL
                     QSRRAVAPLVVSRIKVMSKLDIAEKRLPQDGRISLRIAGRSVDVRVSTLPSGHGERVV
                     MRILDKQAGRLHLSALGMDPKTEQRIAELFRKPHGIILVTGPTGSGKTTTLYAGLEHL
                     NDNTQNIMTVEDPIEYYIDGIGQTQVNTKVEMTFARGLRAILRQDPDVVMVGEIRDLE
                     TAQIAVQASLTGHLVLSTLHTNTAVGALTRLRDMGIEPFLLSSSLIGVVAQRLVRTLS
                     PDNKVPFEAGEYERRLLGLGPDDPSPTLYRPGPGAGGFRGRTGIYELVIVDDQMRTMI
                     HDEAAEHELERYARTMTPSIRDDGLDRVLRGETTVEEVLRVTRED"
     gene            5015..>5669
                     /locus_tag="VEW08_13540"
     CDS             5015..>5669
                     /locus_tag="VEW08_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYJ41810.1"
                     /translation="MGAFEYTALDTAGREQNGVVEGDTVKHVRQLLRERKLLPVTVAE
                     VKHGEAKRDRSFSLRRGISASDLSLLTRQLATLVKAGLPLEEALLAVSQQSEKPRVQS
                     IMLGVRSKVMEGHSLADGVAEFPKVFPEIYRSTVAAGESSGRLDGILERLADYTENRE
                     VTRQKVLGAMLYPIVLSIICLGIVTGMMVYVVPKVIEVFESSKGKLPFATQVLIAVSE
                     "
BASE COUNT         1122 a         1881 c         1748 g          918 t
ORIGIN      
        1 tgatgatggt cacgagcgta ggaggtcacc agggccactt tggtttccgc tatttcatat
       61 gtgaacacac cgcagatgcc tttgccctgc gtatgtacct cgagcatgac gcgtgtcgca
      121 gtcaggcggt ccatacgaaa gaacttctcg agcacgtcga cgacaaactc catgggtgtg
      181 taatcgtcgt tgagcagcac cacctgatat aagggcggct gcttggtttt gggctcggct
      241 tcttcgacgg cgagacccgc gcccccttga tcggggcgtt cggtttcaga catgggcatt
      301 ggcgctcttt atatgggtcg tcgattggca acacaagtgc gctgccggtg taggcggatt
      361 gccaagacaa aacaaagcct tgaggctgct gcagagcgaa acgtatcatc atggaaatga
      421 cggcaaactt ctggaatccg ggcgcgagca gcgggggcga ctggcgctcc tcgctgctgg
      481 cacgcgcgcc gcaggttctg acctttgtcc ttgcgctggc gctggccgcg cagctcgctt
      541 tcatggtggt ggccatgacc agccgcagcc gccaggcggg gccgccgccg ctcgcggcgt
      601 tgccgccacc cgcgcaactc gacatcgggg ggctggtgaa cgcacatttg ttcggtaacg
      661 ccgccgttca ggcgaacggc gacgcggcga atgcgccccc atcgagcatg ccgctggtgc
      721 tggcgggagt tctggcctct gaagatccca aggaaggtat ggcaatcatt ggagagagcg
      781 cccaggcggc aaaggttgtc gccgtcggcc agcaggtacc gggcggcgca caattgcatt
      841 cggtatacag cgatcgcgcc gtcatcgatc gcggcggcac gctcgaatcg gtgttcctgc
      901 caaggcagac cgggggtggg atcgtggcgt ctgcgccgcc gcccatgccc acggcggccc
      961 ccgaaggcaa cgaagccatg atcgagcgca tgcgcaagct ggtgaacgac gacccgggtc
     1021 tgctcagcca ggtcatgcga ccacagccgg tcttcgcggg cggtgaaatg aagggcttcc
     1081 gtgtctatcc cggggcgaat cgccaggcgt tttcacgcat ggggctgcgt gcgggtgaca
     1141 tggtcacggc catcaacggc acgcctctca acgacaagga ccgtgcgcag gaaattttcg
     1201 gcacgctcgg gtcttcgacc gacgcacgcg tcaccattac gcgcaacggc cggcaacaag
     1261 agctcgtgtt gaatatcgcc cagatcacgg ccgaagcgga acgcctcggc accggcggag
     1321 atggcaatat cccgatggaa caggctcccg agccggtgcc cgggaacgaa tgatccggga
     1381 cataacaata atgtttaaaa caagctcgag ttccgggccc agggtactta aagcaatgac
     1441 cagaaaatcc ttccgggtaa cgctgctcgc cgccctgttc gctttcgccg cgactggcat
     1501 cgtcagtgcc caaaacaacg gtcagcgcat cacgcccagt ttcaaggatg ccgaaatcgg
     1561 ccaggtcatc gaggccgtcg cggccgcaac cggcaagacc atcattccgg acccgcgcgt
     1621 gcgcgcgcag gtcacgatgc tgtcgcagac gccgatgacc cccgacgcct tttacgaagc
     1681 gttcctggcg ctgctctcgg tgcaccagtt cgtcgccgtg gaatccggcg gcatcatcaa
     1741 gatcgtgccg gacgccaacg cgcggcagat gccgaacatc gacctgccct cgcgcgtgaa
     1801 gtcgggctcc gatgagctga tcacgcaggt catcgccgtg cacaacgtga atgccgcgca
     1861 actcgtgccg gtactgcggc cgctcatgcc gcaggcggcg cacatggccg cctatcccaa
     1921 cggcaacatt ctcatcctct ccgaccgcgc cagtaacgtg agccgcgtga tgcgcatcat
     1981 cgagcgcatc gaccagcagg gcgacaacga cgtcgacatc atcaacctgc aacacgcgtc
     2041 gtccagcgaa gtcgtgcgcg tggtgaacac cttcttcgcg caacaggggc aggccgaggg
     2101 cggcggcggt tccccgtcgc gtgtcatcgc cgacgaccgc tccaacagcg tgctggtcgg
     2161 cggcgaaaaa tccctgcgcc tgcgcatcaa ggcgctgatc gcgcatctcg atacaccgct
     2221 ggaaagcggc ggtgacacgc aggttcgtta cctgcagttc gccgacgcgg aaaagatcgc
     2281 cgccaagctc aaggaacaga tcagcgcgac cgtggccatt accaccggcg cgccgccggc
     2341 gggcggtggc gccacgagcg gtgtcgccgc cagcgcagat cgctcgacca ccatctgggc
     2401 ggagccggag accaacgcgc tgatcatcac cgcgcccgcc aagatcatgc gctcgctcat
     2461 gagtgtggtg gacaaactcg atatccgccg catgcaggtc ctcgtcgaag ccatcatcgt
     2521 cgacatcaac gtgaacaaga acgccgagct cggcgtgaac tgggccgtgt ggtcggacga
     2581 cgatgacacg cgctttccgg tgggcacgtt tcagcagccc gtcggcggtg tgaacctcac
     2641 gtcgctggcc cagtcgatcg acaaccccag tggtggcaac ccattgctcg gcactggcac
     2701 cacgttcggc atcggccgca tcgccacctc cggcgtgaac ttcgccgcca tgttgcgcgc
     2761 catccagggc gattcgaata ccaacgtcat tgccacgcca tctaccgtga cgatggacaa
     2821 ccaggaagcc gagctgaagg tcgcgcagga agtgccgttc gtcacgggct cctacagcaa
     2881 cacgggcaat accggcaaca acggcgaagt gaatccgttc accacggtcc agcgcgaaga
     2941 agtcggcacc attctcaaga tcacaccgca gatcaacgca cagggcgcgc tggtgcagat
     3001 gaaaatcgag ctcgaaagct cctcgctcac cggagactcc ggcgacgcga acagcctcat
     3061 caccaacaag cgcacgatca gcaccaacgt gctcatcgaa gacggcggca tcatcgtgct
     3121 gggtggtttg atccaggaca gcgacatccg cggcgagcag cgcgtgccct atctcggtcg
     3181 catcccggtg ctcggcgcgc tgttcaagac gcgcagccgc cagaccgaca agaccaacct
     3241 catggtcttc atccgtccga agattctgta cgatggtacg cagacggcgg tcgagacgaa
     3301 cgcgaagtac aactacatcc gcgatgagca gcgcgccgcg ggccggccca agtccgaagt
     3361 gctgccgttg ttgccgtata gcaaggagcc gcgcctgccg ccccttgcgc ccccggaggt
     3421 tccgatgccc ccgcctctcc ccacggacag tgcgacaggc acgacgcctt ccaggccacc
     3481 cgcgaatccc gaaggccccc cgcccaaccc atgaacgagg tcgctgaacc cggggtcgac
     3541 cgtccgaagc gtgtcggctt tgccttcgcg aagcgccacg gcatcctcgt cgaccgaatt
     3601 catgaaggcg tcgcagagtg cgtatacaga cggggtaccg caccgctggc ggtggccgaa
     3661 gtgcggcgtt atctcggcat tccgatccgg ctcgagcggg ttgaagacga aaagttcgac
     3721 tcactcctgc ggattgcctt cgaagccggt aacgacgcca tgcaggccgc cgaaggtgtc
     3781 gaggaatcca ccgatctcgc ccatctcgcg caggacctgc ccgaacaggc cgacctgctc
     3841 gagagcgaag acgacgcacc gatcatccgg ctcatcaacg cggtgctgac gcaggccgtc
     3901 aaggaaaacg cgtccgacat ccacgtcgaa cccttcgaaa accggctggt ggtgcgcttc
     3961 cgcgtcgacg gtgtcctgcg tgaggtgctg cagagccggc gcgcggtcgc cccattggtc
     4021 gtgtcgcgca tcaaggtcat gtccaagctc gacatcgccg agaagcggct gccgcaggac
     4081 ggacgcatca gcctcaggat cgccggccgc tcggtcgacg tacgcgtgtc gacactgcca
     4141 tcggggcacg gcgagcgcgt ggtcatgcgt atcctcgaca agcaggccgg aaggctgcac
     4201 ctctcggcgc tcgggatgga cccgaagacc gagcagcgca tcgccgaact attccgtaaa
     4261 ccgcacggca tcatcctggt gacgggcccc acgggctcgg gcaaaacgac gacgttgtac
     4321 gccggcctcg agcacctgaa cgacaacacg cagaacatca tgacggtcga ggatccgatc
     4381 gagtactaca tcgacggcat cggccagact caggtcaaca cgaaagtgga gatgaccttc
     4441 gcgcgcgggc tgcgcgccat cctgcgccag gatccggacg tggtcatggt cggcgaaatc
     4501 cgcgacctcg aaaccgcgca gatcgcggtg caggcctcgc tcaccggcca cctggtgttg
     4561 tcgacgctgc acacgaacac ggccgtcggc gcactcacgc gcctgcgcga catgggtatc
     4621 gaacccttcc tgctgtcgtc gagcctgatc ggcgtcgtcg cgcagcggct ggtgcgcacg
     4681 ctcagtcctg acaacaaggt gccgttcgag gccggtgaat acgaacgccg gctgctcggg
     4741 ctcggacccg atgacccctc gcccacgctg taccggcccg gtcccggcgc cggcggcttc
     4801 cgcggccgta caggaattta tgaactcgtc atcgtcgacg accagatgcg cacgatgatc
     4861 cacgacgagg ccgccgagca cgaactcgag cgctacgcac gcacgatgac gccctccatc
     4921 cgcgacgatg gcctcgatcg cgtcctgcgc ggcgaaacca cggtcgaaga agtcctgcgc
     4981 gtcacgcgcg aggactgagc cgggggccgg atccgtgggc gctttcgaat acaccgctct
     5041 cgacaccgcg ggccgcgaac agaatggcgt cgtggaaggc gacaccgtca agcacgtacg
     5101 ccagctcctg cgcgagcgaa aactgctgcc ggtcaccgtg gccgaggtca aacacggtga
     5161 agcgaaacgc gaccgcagtt tcagccttcg gcgcggcatt tccgcatccg atctgtcgct
     5221 gctcacgcgc cagctcgcca cgctcgtgaa agccgggctg ccgctcgagg aagcgctgct
     5281 cgccgtctcc cagcaatcgg aaaagccgcg cgtacagagc atcatgctcg gcgtgcgcag
     5341 caaggtgatg gaaggtcact cgctggcgga tggcgttgcg gaattcccca aggtcttccc
     5401 cgagatctat cgctctaccg tggccgccgg cgaaagctcc ggccgcctcg acggcattct
     5461 cgaacgcctt gccgactaca ccgaaaaccg cgaagtcacg cggcagaagg tgctcggcgc
     5521 catgttgtac cccatcgtgc tgtcgatcat ctgcctcgga atcgtcaccg gcatgatggt
     5581 ctacgtggtg ccgaaggtca tcgaggtgtt cgagtcgagc aagggcaaac taccgttcgc
     5641 cacccaggtg ctcatcgccg tcagcgaat
//
LOCUS       DASQJS010000339         6645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_212824, whole genome shotgun sequence.
ACCESSION   DASQJS010000339 DASQJS010000000
VERSION     DASQJS010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212824"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VEW08_13545"
     CDS             <1..1210
                     /locus_tag="VEW08_13545"
                     /inference="COORDINATES: protein motif:HMM:NF004784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HYJ41811.1"
                     /translation="DVRGGRMSQEEFTAAESCMHRSKGHCMTMGTASTMASMVEALGV
                     ALPGNAAIPAVDSRRYRQAQLTGRRIVEMVEEEMVLSKILTRAAFENAIRVNAAIGGS
                     TNAVIHLLAIAGRIGVPLKLEDWDKIGSELPCLLNLQPSGKYLMEDFYYAGGLPAVMK
                     EIAHVLDGKALTVNGKTIGENIADAPCWNRDVIKGLSQPFRDRAGIAVLRGNLAPDGA
                     VIKPSAASPHLLRHKGRAVVFENIEDLHARIDDENLDIDEHCIMVLKGCGPKGYPGMA
                     EVGNMPLPPKLLRRGITDILRISDARMSGTAYGAVVLHVSPESAAGGPLALVRNGDLI
                     EVDVPARRLHLDVSETELAARRASWKPAPAPTRGWYKLYVDHVQQAHLGADLDVLVGG
                     SGSDVSRDAH"
     gene            complement(1345..4866)
                     /locus_tag="VEW08_13550"
     CDS             complement(1345..4866)
                     /locus_tag="VEW08_13550"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYJ41812.1"
                     /translation="MSPWPRWRAYVLAIAFTLAVLLMRVLAEPWMGHRPFLLVFLIPV
                     ILAAYLSGLRPGLFATALAGLATKLFVFPPFWSAWFNDPLDFAHWLFLLLVGVLLSVL
                     FDELQQWRDKTAARSTERQHATTERKIRVGFGIALAFLGAIGIVSYLSVSRFTENAQL
                     VAHSQLVMSNIDALVTTTFETESAQRAYVLTGEEPFAAEYTRAMGRVEGLVQQLRDAV
                     STMPAQLARVEPLAEVVRARMKNSSELIELRRSGGMEAVQLRLGQTANRPGVNLQSRI
                     RALAREMKVAEIELLNDREREANQSAQVTQSVIVGGSVLALIFVSLALYAIRRDIAGR
                     ARAESELNRFFDLSIDMFTIASPDGRFKRMSRAVTDMLGYTMDEALSLDYMEMQHPDD
                     RARTVEVVDAQINRGERVENFESRFRHKDGTYRMLSWRSNPAGGLMYATARDVTAAHA
                     AAEALLEAKGQLEVRVEQRTRELAQANESLRKSERRFRALIEHGSDCIAMVDTDGRIL
                     YVSPAVTTVEGYTPEELLGKPRTEQTHPDDLPTLAAATEKLRAQPGKPIPAIWRRRHK
                     DGRWIWLEGVATNLLDDPSVGAIVTNYRDVTERLEHETRLGEQMRRLALMARITRAIG
                     ERQDLRSIFQVVVRSIEEELPVDFCMIGLYEAGENQLTVSCVGARTGPVATRLDMIEG
                     TNIPIDENGLSRCIQGQLVHEPDIADSPFPFPKRLASAGMCSLVIAPLLVESQVFGVL
                     VTARHTANAFSSGECEFVRQAMEHTALAAHQAQLYTALQQAYDDLRTSQQQIMQQERL
                     RALGQMASGIAHDINNAISPVALYTEALLEREPNLTDRGRSQLEVIQRAVDDIAQTVA
                     RMGEFYRLREPLLTLMPVDLNKLVGQVIDLTRARWSDMAHQRGAAIDMRTEFDRNLPL
                     VSAVESQIRDALVNLVFNAVDAMPEGGPLIIRTCLAKGSQEQIVLLEVQDAGVGMDED
                     TRRRCLEPFFTTKGTRGTGLGLAMVYGVAQRHGASLEIESEIGKGTLVRMTFAIAPSA
                     AAVSSGRQRQPAGPMRILVIDDDPLLLKSLRDTLEVDGHEVITANGGQAGIEAFVESH
                     AEGRPFPVVITDLGMPHVDGRKVAATIKASVPATLVLMLTGWGRRLVVEGDVPPGVDQ
                     VLSKPPKLLELRAALARTGAGPGNREN"
     gene            complement(4971..>6645)
                     /locus_tag="VEW08_13555"
     CDS             complement(4971..>6645)
                     /locus_tag="VEW08_13555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ41813.1"
                     /translation="ALQWIDSTYHVRWIEPLAGNESELDRDMLTDEGTGALRAAGELG
                     HELLTRPAPMAQGHVGFFAYLPVRRAGRFDGFIAGQFSVEDLFNGAVDTRLSADYSAS
                     ILHDAATIFANSRGDSPLTRDLATEKNLRIGDRTWTLRMTPTPDFVERQKSGLPLLVL
                     GAGLLVAALAALSVRYVLISRLKSAHLAKSLALNAGIISSSAHLVIAIDAEYRIMIFN
                     RAAEKALGYSAREVVGRRAIPIFMDPAELMERARSLSAELGENVPLGPEILTRIPLRD
                     GLETRTWTFVRKDGTRFPTNVIITPLRDEQGATAGFLGVIEDVSSRHEVDRMKSEFTA
                     VVSHELRTPLTSIRGSLGLILGAMAGSLPPKVRDLLEIAQSNSDRLVLLINDILDIEK
                     FSAGQMRFDIATLSLGDIVRQAVESNEGYARKHHVNIALAAVDPAFMVAVDPDRFVQV
                     LTNLLSNAVKYSPASGTVHVAAERTGDAVRVSVRDSGPGIPENFRARIFERFSQADAS
                     TTREKGGTGLGLYIARRFIEQMQGRIGFDSETGKGATFWVELPAAPRNA"
BASE COUNT         1146 a         2106 c         2210 g         1183 t
ORIGIN      
        1 agacgtgcgc ggcgggcgca tgtcgcagga agagttcacg gccgccgagt cctgcatgca
       61 tcggtcgaag ggacactgca tgaccatggg cacggcatcc accatggcga gcatggtcga
      121 agcgctgggc gtcgcgctgc cgggcaatgc ggcgattcct gccgtcgatt cacgccgtta
      181 ccggcaggcg cagctcaccg ggcggcgcat cgtcgaaatg gtcgaagaag agatggtgct
      241 gtcgaaaatt ctcacgcgcg ctgcattcga gaatgcgatc cgggttaacg ccgccatcgg
      301 tggctcgacc aatgcggtca ttcacctgct ggcgattgcc gggcgcatcg gagtcccgct
      361 caagctcgaa gactgggaca agatagggtc ggagctgccc tgtcttctga atttgcagcc
      421 gtccggcaaa tacctgatgg aagatttcta ttacgccggt ggactgccgg cagtgatgaa
      481 ggaaattgcg cacgtgctcg atggcaaggc gctgaccgtc aatggcaaga ccatcggcga
      541 gaatatcgcc gacgcaccgt gctggaatcg cgacgtcatc aaagggctga gtcaaccttt
      601 ccgcgaccgc gcgggcatcg cggtcttgcg cggcaacctg gcccccgatg gcgcagtcat
      661 caagccgtcg gcggcgtcac cgcacctgtt gcggcacaag gggcgcgcgg tggtcttcga
      721 gaacatcgaa gacctgcacg cgcgcatcga cgacgagaat ctcgacatcg acgagcactg
      781 catcatggtg ctcaagggat gtggcccgaa gggttatccc gggatggcgg aagtgggcaa
      841 tatgccattg ccgcccaaac tactgcgccg tggcatcacc gacatcctgc gcatctccga
      901 tgcgcgcatg agtggcaccg cttatggcgc cgtggtgctg cacgtctcgc ccgaatccgc
      961 ggccggtggc ccgctggcgc tggtgcgcaa cggcgacctg atcgaagtgg acgtacccgc
     1021 acggcgcctg catctcgacg tcagtgaaac cgaactcgcg gcccggcgcg ccagctggaa
     1081 gccggcgccg gcgcccacgc gcggctggta caagctgtac gtggaccacg ttcagcaggc
     1141 gcacctcggc gccgatctcg atgtgttggt tggcggcagc ggatcagacg tttcgcgcga
     1201 cgctcactag cggtgccggg ccgggaaacc gggaaaactc ataggttcgc gcaatacttg
     1261 cgacagcgaa aagttttccc ggtttcccgg cccggcaccg gtccgtggac ctcgctgcac
     1321 ggtgagttcg gtggaacggc gagattagtt ttcccggttt cccggcccgg caccggtccg
     1381 tgcgagcgcg gcgcgcagct cgagcagctt gggcggcttg ctcagcacct gatcgacgcc
     1441 gggcggcaca tcgccctcga cgaccagacg ccggccccag cccgtgagca tcaacaccag
     1501 cgtcgccggc acgctggcct tgatcgtcgc ggccaccttg cggccgtcca cgtgcggcat
     1561 gccgaggtcg gtgatgacca cgggaaacgg cctgccctcg gcgtgtgact cgacgaacgc
     1621 ctcgatgcct gcctggccgc cgttcgcggt gatgacctcg tgcccgtcca cctccagcgt
     1681 gtcgcgcagg gacttcagca gcagcgggtc gtcgtcgatg acgaggatac gcatgggacc
     1741 cgcgggttga cgctgccggc cgctcgacac cgcggctgcg gagggcgcga ttgcgaacgt
     1801 cattcgtacc agcgtgccct tgccgatctc gctctcgatt tcgaggctgg ctccgtgtcg
     1861 ctgggcaaca ccgtaaacca tcgccagccc caggccggtg ccgcgcgtgc ccttcgtggt
     1921 gaagaaaggt tcgagacagc ggcggcgcgt gtcctcgtcc atgcccacac ccgcatcctg
     1981 cacttcgagc aacacgatct gctcctggct tcccttggcg aggcacgtgc gaatgatgag
     2041 ggggccgcct tcgggcatgg cgtcgacggc attgaatacg agattgacga gcgcatcgcg
     2101 aatctggctc tcgacggccg acaccagcgg cagattgcgg tcgaactcgg tgcgcatgtc
     2161 gatggccgcg ccgcgctgat gcgccatgtc gctccagcgc gcgcgcgtga gatcgatgac
     2221 ctggccgacc agcttgttga gatcgacggg catcagcgtg agcaggggct cgcgcaggcg
     2281 atagaactca cccatgcgcg cgacggtctg cgcaatgtcg tccaccgcac gctggatgac
     2341 ttcgagttgc gagcgcccgc gatcggtcag gttgggctcg cgctcgagca atgcttcggt
     2401 gtagagagcc accggcgata tcgcgttgtt gatgtcgtgt gcgataccac tggccatctg
     2461 tcccagtgcg cgcagacgtt cctgctgcat aatctgttgt tgcgaggtac gcaggtcgtc
     2521 ataggcctgc tgaagggcgg tgtacaactg cgcctggtgc gcggccagcg cggtgtgttc
     2581 catggcctgg cgcacgaact cgcactcgcc gctgctgaac gcgttggcgg tgtgccgcgc
     2641 cgtgaccagg acaccgaaaa cctggctctc gaccagcagc ggcgcgatga ccagcgaaca
     2701 catcccggcg ctcgccagcc ttttcggaaa cgggaaaggt gaatccgcga tgtccggctc
     2761 gtgaaccagc tgaccctgga tgcaacgcga caggccgttc tcgtcgatgg gaatgttcgt
     2821 gccttcgatc atgtcgagcc tggtcgccac cggtccggtg cgggcgccga cgcagctcac
     2881 cgtgagctgg ttctcgccgg cttcgtacag gccgatcatg cagaagtcga ccggcagttc
     2941 ttcttcgatg ctgcgcacca cgacctggaa gatgctgcgc agatcctggc gctcgccgat
     3001 ggctcgcgtg attcgcgcca tcagcgccag ccttcgcatc tgctcgccga gcctcgtttc
     3061 gtgctccagc cgctcggtga cgtcgcggta gttggtgacg atggcgccca ccgatggatc
     3121 gtccagcaaa ttcgtggcga ctccctcgag ccagatccag cgtccgtcct tgtggcggcg
     3181 gcgccagatc gcggggatgg gcttgcctgg ctgcgcgcgg agtttttcgg tggcggccgc
     3241 gagggtcggc aggtcgtccg ggtgggtttg ctcggtccgc ggcttgccta acaactcttc
     3301 aggcgtgtaa ccctcgaccg tggtgaccgc cgggctcacg tagaggatcc ggccgtccgt
     3361 gtccaccatg gcgatgcagt ccgaaccgtg ctcgatgagc gcacggaacc gcctttcgct
     3421 cttgcgcagg ctttcattgg cctgcgccag ttcgcgcgtg cgttgctcga cgcgcacttc
     3481 gagctgccct ttggcttcga gcaaggcctc ggcggcggca tgagcagcgg tgacgtcgcg
     3541 tgcggtggcg tacatgaggc cgccggccgg attcgagcgc cacgacaaca tgcgataggt
     3601 gccgtccttg tggcggaacc gtgactcgaa gttctcgacg cgctcgccgc gattgatctg
     3661 cgcgtccacg acctcgacgg tacgggcgcg atcgtcggga tgctgcattt ccatgtagtc
     3721 gaggctcaat gcctcatcca tggtgtagcc gagcatgtcc gtcaccgcgc ggctcatgcg
     3781 tttgaagcgg ccgtcggggc tggcgatggt gaacatgtcg atggacagat caaagaagcg
     3841 attgagctcg gactcggcgc gcgcacggcc ggcgatgtcg cggcggatgg cgtagagcgc
     3901 aagcgacacg aatatgagcg cgagcacgct gccgccgacg atgaccgatt gagtaacctg
     3961 ggcgctctga ttcgcctcgc gctcgcggtc gttcaacagc tcgatctccg ccaccttcat
     4021 ctcccgcgcg agcgcgcgga tgcgggactg gagattcacg ccgggccgat tcgcggtctg
     4081 gccgagtcgt agctgaacgg cttccatgcc gccgctgcgc cgcagctcga tgagctcgga
     4141 actgtttttc atgcgcgcac gcacgacctc cgcgagcggc tccacgcgtg caagttgcgc
     4201 cggcatcgtg ctcacggcgt cgcgcaactg ctgcacgagg ccttcgacgc gacccatggc
     4261 gcgggtgtat tcggcggcaa agggttcctc gccggtgagc acgtaggcac gctgtgcaga
     4321 ttcggtttcg aaagtcgtcg tgaccagcgc gtcgatgttc gacatcacca gctgcgaatg
     4381 cgcgacgagt tgagcgttct cggtgaatct cgacacgctc agataactga cgatgccgat
     4441 ggcgcccagg aatgcgagtg cgatgccgaa gccgacgcgt atcttgcgct cggtggtcgc
     4501 gtgctggcgt tcagtgctgc gcgcggccgt cttgtcgcgc cactgctgca gctcgtcgaa
     4561 cagcacgctc aggagcacgc caaccaggag caggaacagc caatgcgcga agtccaacgg
     4621 atcgttgaac caggcgctcc agaacggcgg gaacacgaac agtttggtgg ccagtcccgc
     4681 cagcgcggtg gcgaaaaggc ccggacgcag gccgctcagg taggcggcga gaatgaccgg
     4741 gatgaggaat accagcagga acggccgatg acccatccac ggctcggcca acacgcgcat
     4801 cagcaacacg gccaacgtga acgcgatggc gagcacgtac gcgcgccagc gcggccatgg
     4861 gctcaacgga ggaggtgatg cctcattctg cactgccgca gagtaatctc ccaggcaggg
     4921 gtggggaagt gggaagtccg ctattgctga ttcccgtggg cgatcagtcg tcaggcatta
     4981 cgcggagcgg ccggcagctc cacccagaaa gtcgcgccct tgccggtctc ggaatcgaag
     5041 ccgatccggc cctgcatctg ctcgatgaac cgccgcgcaa tgtaaagacc gagtccggtg
     5101 ccgcctttct cgcgcgttgt cgaggcatcg gcctgtgaaa atctctcgaa aatgcgcgcg
     5161 cggaagttct ccgggattcc gggtccgctg tcgcggacgc tgacccgcac cgcgtcgccg
     5221 gttcgttcag ccgcgacgtg tacggtcccc gaggccggcg agtacttgac ggcattcgac
     5281 aacaggttgg tcagcacctg tacgaagcga tctggatcga cggcgaccat gaatgccgga
     5341 tcaacggcgg cgagcgcgat gttgacatgg tgcttgcgcg cataaccctc gttcgactcg
     5401 accgcctggc ggacgatgtc gccgagcgac agtgtcgcga tgtcgaagcg catctgcccg
     5461 gcgctgaact tctcgatgtc gaggatatcg ttgatgagca gcaccaggcg gtcggagttg
     5521 ctctgtgcga tctccagcag gtcgcggact ttcggcggca gactgccggc catggcgccg
     5581 agaatcaggc ccagcgatcc gcggatggag gtgagcggtg tgcgcagctc gtggctgacc
     5641 accgcggtga attcgctttt catgcgatcg acttcgtggc ggctgctcac gtcctcgatg
     5701 acgccgagga atccggcggt ggcgccctgt tcgtcgcgca gcggcgtaat gatgacgttc
     5761 gtagggaacc gcgtgccgtc cttgcgcacg aaagtccatg tccgagtctc gaggccatcg
     5821 cgcagcggaa tgcgcgtcag gatttcggga cccagcggga cgttctcccc cagctcggcc
     5881 gagagcgagc gcgcgcgctc catcagttcc gccgggtcca tgaaaatggg aatggcgcgc
     5941 ctgccgacca cttcccgcgc ggaatatccg agcgccttct cggcggcgcg gttgaaaatc
     6001 atgatgcggt attccgcgtc gatcgcgatg accaggtggg cgctgctcga aatgattccc
     6061 gcgttgagtg ccagcgactt tgcgagatgc gcagacttga ggcgggagat gagcacgtaa
     6121 cgtaccgaga gggccgccag cgccgccacc agcaggccag cgccgagcac cagcagcggc
     6181 agcccggatt tctgccgctc cacgaaatcg ggagtcggcg tcatgcgcag cgtccacgta
     6241 cgatctccga ttcgcaggtt cttttcggtt gccaggtcgc gcgtcagcgg gctgtcgccg
     6301 cggctgttgg cgaatatcgt cgccgcatca tgaagaatgg aggccgagta gtcagccgac
     6361 aggcgggtat ccacggcgcc gttgaaaaga tcttccaccg agaactgccc cgcgatgaag
     6421 ccatcgaatc ggccggcgcg ccgcacaggt agataggcga agaagccgac atggccctgc
     6481 gccatcggcg ctggtcgtgt cagcagttcg tggccgagct cacccgcggc gcgcagcgca
     6541 ccggtgcctt cgtcagtcag catgtcgcgg tcgagctcgc tttcgttgcc ggccagcggc
     6601 tcgatccagc gcacgtgata ggtggagtcg atccactgca gcgcg
//
LOCUS       DASQJS010000340         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3037132, whole genome shotgun sequence.
ACCESSION   DASQJS010000340 DASQJS010000000
VERSION     DASQJS010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3037132"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1226)
                     /locus_tag="VEW08_13560"
     CDS             complement(165..1226)
                     /locus_tag="VEW08_13560"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HYJ41814.1"
                     /translation="MMQTGELLVIVPVSDDALSRIAAVDRCLHVVDARGWFDVELRET
                     WPPWTVQRYLGRPAGPITSRQERDQRLARAEIILGGFPFPLDLRARAPSLRWFHQLPA
                     GASNLLGGDLWGSDVVVTTSRGYGNTRPMAEYVLASLLHFARGLHHASRDRQRHRFDH
                     RTYRPLLLQGKTLCVVGAGGIGQEVGRLCASAGMRVIGTRRRVAPDAVLPDGFSRLES
                     PARLCALLGESDAVAVCCQWTPETTKLIGREAFAAMKPGTILVNVARGEIIDEEALIA
                     ALAAGTLRGVALDVYVGEFEREPDRRLWDDERVLITPHISGGTDLHQHRGITLFCDNL
                     RAYLDGRPLTNVVDWADGY"
     gene            1357..>2418
                     /locus_tag="VEW08_13565"
     CDS             1357..>2418
                     /locus_tag="VEW08_13565"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYJ41815.1"
                     /translation="MNDVTTKDLTEREFKVVGTRPPRPDGVDKVTGRAKFGADLHVAN
                     MLVGKVLRSPHAHARIKAIDTSKALALHGVKAVITAADFSDLPSEFIPAGEMMVNFRD
                     MTRNIMAREKALYEGHAVAAVAATSEAVAAQALELIDVDYEVLPHVIDVEAAMAPGAP
                     VLHDNMFTIGVDPKPAQPSNVAKRVEFNLGNIEAGFAQADVVVEREFTTTPVHQGYIE
                     PHACLASVSEDGQAELWCTTQGQFIVRSFCAKLLGMELAKLRVTPSEIGGGFGGKTVV
                     YLEPLALALSRKAGQPVKMVMSRTEVFQSTGPTSGSKVWVRIGATRDGRITAADALLK
                     YQAGAFQGSPVQPGCMCAFA"
BASE COUNT          451 a          801 c          781 g          385 t
ORIGIN      
        1 tcgtcaatat caagaccgcg aaagcaatcg gactcacgat tcctcagggc ctgctggctc
       61 gggcagacga ggtgatcgag taagtgcgcg acgcgcctgc acgcatctgg gacgcacgcg
      121 cctgcggctc cccgcccacc gcagggtcag cgcttcacca ttggtcaata cccatccgcc
      181 caatcgacga cgttggtcag gggccgcccg tccagataag cgcgcagatt gtcgcagaag
      241 agggtgatcc cccgatgctg atgcaggtca gtgccgccag agatgtgcgg agtgatgaga
      301 acgcgctcgt cgtcccagag gcgacgatcg ggttcccgtt cgaactcgcc cacatagacg
      361 tcgagggcca cccctcgcag cgttcccgcc gcaagggcgg cgatcaacgc ttcttcgtcg
      421 atgatttcac cccgcgcgac gttgaccagg atggtgcctg gcttcattgc ggcaaaggcc
      481 tcccgaccga tcagcttggt cgtctccggg gtccactgac aacagaccgc cacggcatcg
      541 ctctccccga ggagcgcgca gagacgcgcg ggactctcca ggcggctgaa cccgtccggc
      601 aatacggcat cgggcgccac ccgtcgccgc gtgccgatca cccgcatgcc ggcactggcg
      661 cacaaccgcc ccacctcctg gccgatgcca ccggcgccca cgacgcacag cgtcttcccc
      721 tgcagcaaca gcggacgata ggtgcgatgg tcgaaacgat gccgctgccg atcgcgcgat
      781 gcatggtgga gccccctcgc gaagtggagg agactggcta gcacatactc ggccatgggg
      841 cgggtattcc catagccgcg cgaggtggtg acgacgacat cgctgcccca gagatccccg
      901 cccaacaggt tgctcgcccc ggccgggagc tggtggaacc agcgcaggct cggcgcccgg
      961 gcccggagat ccagcggaaa cggaaagcca ccgagaatga tttcggccct ggccagccgc
     1021 tgatcgcgct cctggcgcga agtgattggt cctgcgggcc ggccgaggta gcgttgcacg
     1081 gtccacggcg gccaggtctc gcgcagttcg acgtcaaacc agccccgcgc atcgaccacg
     1141 tgcaggcatc ggtcgaccgc cgcgatgcga ctcagcgcgt cgtcggatac cggcacgatg
     1201 accaggagct ctccggtctg catcatcgat ggatgtcgct tacccccttg gtgggcagcc
     1261 attatgccaa gtcaaccaaa atgtgcagtc ccatgaagaa tggtgtttac ttggcaattc
     1321 ctatcgggcc gttggccgca cgcaaggagt tgagcgatga acgacgtcac gacgaaagac
     1381 ctgaccgagc gcgagttcaa ggtggtgggc acccggccgc cgcgccccga cggggtagac
     1441 aaagtcacag gccgcgccaa gttcggcgcc gatctccacg tggcgaacat gctggtgggc
     1501 aaggtgctgc gcagcccaca cgcgcacgcg cgcatcaaag cgatcgacac ctcgaaagcc
     1561 ctggccttgc atggcgtgaa ggcggtgatc accgccgccg acttcagtga tctgccctcg
     1621 gaattcattc cggcaggcga gatgatggtg aacttccgcg acatgacgcg caatatcatg
     1681 gcgcgcgaga aagcgctcta cgaaggccac gcggtcgcgg cggtggcggc gaccagcgag
     1741 gcggtggctg cgcaggcgct cgagctcatc gacgtcgact acgaagtgct gccgcatgtg
     1801 atcgacgtgg aggcagcgat ggctcccggc gcacccgtgc ttcacgacaa catgtttacc
     1861 atcggcgtgg atcccaagcc ggcgcaaccc tccaacgtcg ccaagcgtgt cgagttcaat
     1921 ctcgggaaca tcgaggccgg cttcgcacag gcggacgttg tagtcgagcg ggagttcacc
     1981 accacgcccg tgcatcaggg ttatatcgaa ccgcacgcct gcctcgcgag cgtgtccgag
     2041 gatggtcagg cggagctgtg gtgcacgacg caagggcagt tcatcgtgcg cagcttctgc
     2101 gcgaagctgc tcggcatgga actcgcaaag ctgcgcgtga ccccctccga gatcggcggc
     2161 ggcttcggcg gcaagaccgt tgtctacctc gagccgctcg cgctcgcgct gtcgcgcaag
     2221 gcggggcagc cagtgaagat ggtcatgtcg cgcaccgagg tgttccagtc gaccggcccg
     2281 acttccggct cgaaggtgtg ggtcaggatc ggcgccaccc gcgatggccg gatcacggca
     2341 gccgatgcgc tgctcaagta ccaggcaggg gcgttccagg gttctcccgt gcagcccgga
     2401 tgcatgtgcg catttgct
//
LOCUS       DASQJS010000341         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4291990, whole genome shotgun sequence.
ACCESSION   DASQJS010000341 DASQJS010000000
VERSION     DASQJS010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4291990"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VEW08_13570"
     CDS             <1..1056
                     /locus_tag="VEW08_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HYJ41816.1"
                     /translation="QPGAGLLPEDWLDHEMVAAAVRGVGRSRGSWLLKYGAVEGYLPL
                     REALSTRLGQAGIVVAPEHIVTSAGATEAIDLVARRYLAPGDLVFVEDPAWFVMFARF
                     AAFGARIVGVPRGVDGPDIAALESLLARERPKLFILNSVAHNPTGSCMSAQVAARVLE
                     LANRHDFMILEDDIYGDLQGRHAVRLASLDDGRRVIYVGGFSKTLAANLRVGFVAAHP
                     ALARELRDIKALTGLSSSEIGERVVARIIIERKYERVLEGLRIKLDECRARTAEALLS
                     MGARIAGPPPGGMYLWADFGRDTNELATAGSREGVLCAPGSLFSPTQLPSTWMRVAAA
                     TCLNATAMAFLQRAMRR"
     gene            1309..1593
                     /locus_tag="VEW08_13575"
     CDS             1309..1593
                     /locus_tag="VEW08_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41817.1"
                     /translation="MNLKMGETSQTQVLEVFGAPNITSIDGSGQEVWTYQRAATVSQS
                     TGSSGYWTVLLAGQSQEASGLEQTQRTMTLIIKFNDRKIVSDFRSRSSEF"
     gene            1603..2091
                     /locus_tag="VEW08_13580"
     CDS             1603..2091
                     /locus_tag="VEW08_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41818.1"
                     /translation="MNKIARAVVVASFAVAIAGCVMPPEVARTSLQLQSFQAKEFETS
                     KVAAFGSTLSVFQDLGYIVASADLETGFITAASPAGNKSNVWEVLAGTTSSVQTRATA
                     FVEEIRPGFASIRLNFVEARRLSTMHGQTTDNDRPILEPKVYEAAFEKIGDAIFIRSG
                     VR"
     gene            2168..>2492
                     /locus_tag="VEW08_13585"
     CDS             2168..>2492
                     /locus_tag="VEW08_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41819.1"
                     /translation="MFSASAKRLLLVAGSACLTLAACSKKEEAPAPAAAAAPMASTAL
                     EGALDIVAWPGYIERGETDKAYDWVSKFEAETGCKVTVKTAGTSDEMVTLLTSGNPQP
                     SPPGDA"
BASE COUNT          465 a          790 c          797 g          440 t
ORIGIN      
        1 cagcccggcg cgggcctgct gcccgaagac tggctggacc atgaaatggt cgcggccgcc
       61 gtacgcggcg tggggcgcag ccgtggctcg tggctgctca agtatggtgc cgtcgagggc
      121 tatctaccgt tgcgcgaggc gctgagcacg cggctcggcc aggccggcat cgtggtcgcg
      181 cccgagcata tcgtgaccag cgccggggcc accgaggcca tcgacctggt cgcgcggcgg
      241 tacctcgcgc ccggagacct ggtgttcgtc gaggatccgg cgtggttcgt gatgttcgcc
      301 aggttcgcgg cattcggtgc gcgtattgtc ggtgtgcctc gcggggtcga cggcccggac
      361 atcgcagcgc tcgaaagcct gctggcgcgc gagcggccga agctgttcat cctgaattcc
      421 gtggcgcaca acccgacggg ctcctgcatg agcgcccaag tcgccgcgcg cgtgctcgag
      481 ctcgccaacc gccacgattt catgattctc gaggacgaca tctacggcga ccttcagggt
      541 cgtcacgccg tgcgtctcgc cagcctcgat gacgggcggc gtgtcatata cgttggcggc
      601 ttctccaaga cgctcgccgc caacctgcgc gtcggcttcg ttgcggcgca tcccgcgctg
      661 gcgcgcgaat tgcgcgacat caaggcgctc accgggctgt cctccagcga gatcggcgaa
      721 cgcgtggtgg cgcgcatcat catcgagcga aagtacgaac gcgtgctcga aggtctgcgt
      781 atcaaactcg atgaatgccg ggcgcgcacg gcggaggcgc tactgagtat gggcgccagg
      841 atcgccggac cgccgccggg tggcatgtac ctttgggcgg attttggccg cgacacgaat
      901 gaactcgcca cggccggcag ccgcgaaggg gtgttgtgcg cgccgggcag cctgttctcg
      961 cccacgcagc tgcccagcac ctggatgcgt gtcgcggcgg cgacctgcct caacgcaacc
     1021 gcaatggcct ttttgcagcg cgcgatgcgg cgctgagccg gccggctttg gcggccacat
     1081 ttcgggacga actacccccg aagtgtggtc gccatattcc tgctcagcgt ccgttcaggt
     1141 tcgcgggagt tagataggcg ccccggccaa catgttcgag gcgacgtcat gagatttcac
     1201 gatcttgttg cggccagcgc cgctgtgctc attttctccg cctgtgcccc ggttccgacg
     1261 aagccagtga caactcgaaa cagcgagctg actcacggga atgtccagat gaacctgaag
     1321 atgggcgaga ccagccagac ccaggtcctc gaagttttcg gtgcgcctaa catcacgtca
     1381 atcgacggct cgggacagga agtctggacg taccagagag cggcgacggt gtctcaatcc
     1441 actggttcga gcggctactg gacagtcctg ctcgcgggac agtcccagga ggcgtcgggt
     1501 ctcgaacaga ctcaacgcac gatgacgctg atcatcaagt tcaacgaccg gaagatagtc
     1561 tccgattttc gcagtcgatc gagcgagttc tgagggccgg ccatgaacaa gatagccagg
     1621 gccgtggttg ttgccagctt tgctgtcgcc attgcaggtt gcgtcatgcc gcctgaagtt
     1681 gcacgtacct cgctgcaact tcagtcattc caggccaaag aattcgagac gtccaaggtc
     1741 gccgcattcg gatccaccct gagtgtgttc caggacctgg gatacatcgt cgccagcgcc
     1801 gacctggaga ccggattcat tactgcggcg agccccgcgg gaaacaaatc aaacgtctgg
     1861 gaggtgctgg ccggtaccac gagcagcgtc caaaccaggg caaccgcttt cgtggaagag
     1921 attcgccccg gcttcgccag catccgcctg aattttgtgg aggccaggcg cctgtccacg
     1981 atgcacgggc agacgaccga taacgaccgg ccgatactcg agcccaaagt gtacgaggcg
     2041 gctttcgaga agatcggcga cgcgattttc atacgatcgg gagtccgata acgctgaact
     2101 tgggcctaga atagggacaa agacgccagg cacccattga cccggcgcat acaaaaagag
     2161 gctcgccatg ttctcagcat ccgcgaaacg tttattgctg gtcgcgggca gcgcctgcct
     2221 gacgctcgcc gcctgctcca agaaagaaga agcacctgcg cccgctgccg ctgcggcacc
     2281 catggcgtcg accgcgctcg aaggcgcgct cgacatcgtt gcgtggccgg gctacatcga
     2341 gcgcggagaa accgacaagg cctacgactg ggtctcgaag ttcgaagccg aaaccggctg
     2401 caaggtcacg gtgaaaaccg ccggcacctc cgacgaaatg gtcaccttgc tcaccagcgg
     2461 taacccgcag ccgagtcctc cgggcgatgc tt
//
LOCUS       DASQJS010000342        21659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1465835, whole genome shotgun sequence.
ACCESSION   DASQJS010000342 DASQJS010000000
VERSION     DASQJS010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21659
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465835"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VEW08_13590"
     CDS             <1..681
                     /locus_tag="VEW08_13590"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HYJ41820.1"
                     /translation="ARWGTELHDRFMLPYFVEQDLEDVVAEQNAAGFPLKTEWFAPHF
                     EFRFPKYGDFATLGTEVELRQALEPWHVMGEEGAAGGAVRYVDSSVERLQVLVTGLAP
                     DRYALTCNGRRIPLRSTGTVGEFVAGVRYKAWAPPSALHPLIPAHSPLVFDLVDTWMN
                     RSMGGAQYHVAHPGGRSYDTFPVNAFESESRRLARFVRMGHTPKKVTPVPDLPNIEFP
                     FTLDLRKV"
     gene            703..3129
                     /locus_tag="VEW08_13595"
     CDS             703..3129
                     /locus_tag="VEW08_13595"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HYJ41821.1"
                     /translation="MHAVLEPADLERYDELNDSQGAMRAHWRPLIDRLRADDSPDAVR
                     RSLELTRRLVVENGVTYNVYADPQGTDRPWALDPLPFVLPAAEWKTIEAGVAQRARLL
                     NALLSDLYGPQRLLAEGHLPAELPFGHPNYLWPCHDLRPADGTWLHVYAADLARAPDG
                     RWWLLADRTQAPSGAGYALENREILEQVHPEAIPDMAVRRVRGFFGHLRERMLDAAGD
                     ESPLAVILTPGPYNETYFEHAYLARQMGMPLVEGHDLTVRGDTVFLKTLGGLRRVHSI
                     LRRLDDDYCDPLELRGDSALGVPGLIGAVRAGRVMLSNALGTGVLESAAWLGFLPPIA
                     ERLIGETLLLPEIATWWLGEKPALEYVLANLDRLVIKPTYPNQRFEPMFGREFEGKAR
                     DLLIERLRNRPYAYVAQEHVRLSQAPVWKSAASPELAARALTIRVYAVVTPDGVRVMP
                     GGLARVANEAAADVVSTQRGGGAKDIWVLSDDPHDETITSAQSGKWANLQRQDYIPSR
                     LVENLYWLGRYAVRCENSSRLLLRTLGARADARVWTHARQICRDLGAVSAEGEIFDAL
                     RTRDAQGLQADVKHLAWCASQVRNRLSARYWRGVVGLQRQMQEALATRGSSREVHERV
                     LLSLAALTGFSEEDMMHDEGWRVMRLGRRLERLQFVANILVRQLQSAHATRPEAVEWL
                     LDVCDSTPIYRARYLGVTRLSLMLNLLLYDDGHPMALAFNRRSIDRDLDDLARSLGGE
                     RERGVPDVPLLPQGAAGLLDAQGKEGDAARAALAEELDDLSARSAELSDRLSRRYFAL
                     IESDAQALAT"
     gene            3139..4044
                     /locus_tag="VEW08_13600"
     CDS             3139..4044
                     /locus_tag="VEW08_13600"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HYJ41822.1"
                     /translation="MSRRYRVRHETTYKYAADVMHSHHLLHLVPRPAPYQECLEHEIQ
                     IDPASHRRVDGLDAFGNPLIRLELAQPHRELRVISQMQIEVHSRPVVSLDTTDSWEKV
                     RDSFAYRGAWPSRDQLDAARFRHESPHVRLKQSFTDYSAVCFPEGRPILVCAESLSTK
                     LHTDIEYAPGVTTISTSPTEVVETRRGVCQDFAHLMISCLRSRGLPARYVSGYLRTNA
                     TQSESGEKLVGDAASHAWVAVWGPPYGWIEFDPTNGCFAGTDHIAVAWGRDFGDVSPL
                     RGVILGGADHQLSVTVRVEPEGEPN"
     gene            complement(4041..4634)
                     /locus_tag="VEW08_13605"
     CDS             complement(4041..4634)
                     /locus_tag="VEW08_13605"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HYJ41823.1"
                     /translation="MKRLPLLGLFAVLAAPIVSQEAGGQAPPVVVERPAHWAEPIALE
                     GVPNLHRISPTLYRSEQPTALGMKNIEKLGVRTVINLRYFNDDKDEVEGTSLRTEHTK
                     ILTWRIGDKHVIDVMNMLKKTENGPFLIHCQHGADRTGLMTAMYRILEQGWTPDDALK
                     ELTDGGFGYHSMWKNIIKYVQSVDVEKLKAAIAAAAD"
     gene            complement(4631..5776)
                     /locus_tag="VEW08_13610"
     CDS             complement(4631..5776)
                     /locus_tag="VEW08_13610"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYJ41824.1"
                     /translation="MKLLVLSVSAGAGHVRAADAIVKAAEAAHPKVAATHIDLLSLVP
                     KDFRKLYGEQYIKLVEKLPQLWSFLYSKSDRPSRDSLVGKLKRAAEKLNTRKLTAEIE
                     RLAPDAILCTHFLPAELLSRQRAPGKEQGQKNSAKRLPPLWVQVTDFDVHALWVHPHV
                     DRYCVASEEVAFRLADRGVPREKISITGIPVMPQFSAPLERAVCARELGLSPDRFTVL
                     MMAGGAGVGSLDEMAQRLLRLPGDLQIVALAGRNADLLKRLQALARQHPGKLFPLGFT
                     TTVERVMTAADLVVTKPGGLSVSECLAKERPMLLVSPIPGQEERNADYLLECGAAIKA
                     VDAATLEFKLSRLLTDRPRLAAMSAAAKRVARPRAAADVVALISGSI"
     gene            complement(5807..6421)
                     /locus_tag="VEW08_13615"
     CDS             complement(5807..6421)
                     /locus_tag="VEW08_13615"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYJ41825.1"
                     /translation="MTGPADPSISSGFPTLPPLAPQSPARWRRYLGVLLMRISGWRIA
                     GNIPNVPKYLLIVAPHTSNWDFFHGFCAFLVLRLDNTWLAKHTVFFWPLGILARRFGG
                     MPIDRAKGGNIVRICVAEFARRERMSITITPEGTRGKVKEWKLGFYYIATEAGVPIVP
                     VALDYSKRQVVIMPPFTPTGDAAADLPKIKALYSASMARHPQGF"
     gene            complement(6595..6915)
                     /locus_tag="VEW08_13620"
     CDS             complement(6595..6915)
                     /locus_tag="VEW08_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41826.1"
                     /translation="MIRRTSKYLVLSAMIGFASYGNAGTAAVTPALAACSKALIETIA
                     KTETLPAYKVKAPSRLVSDMVDPNAFTVIARHSRTNELLAKASCKATPAGEIVSFRSI
                     PLKS"
     gene            complement(6964..7911)
                     /locus_tag="VEW08_13625"
     CDS             complement(6964..7911)
                     /locus_tag="VEW08_13625"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HYJ41827.1"
                     /translation="MRTPSLKAIKTFQIAAKHSSFAVAADELCITPSAVSHQIKTLET
                     QLGLPLFSRSARALALTDAGARYLEQIDDLFMRLESVTEQLRARYGRNSVRLHVPAYF
                     ASEMLLPRLSEFSKLHDGIDLRIETQGVHGRTHAAEADISVVVGSGPWGGSLIAHPLF
                     AQTMVPACSPELLLERPVLRYEDLNLHTLLVHEARRDDWDRWATAVGMKNLQPAQIVR
                     IDSMSAATRAAEKSIGIALLPAELSRRKFDSGRLVRVFDDELVTHEEYTLLVRTEDEN
                     RDDIRALCAWLMELAAPMSQSMMNKTDARAESSRLLKQA"
     gene            complement(8342..9028)
                     /locus_tag="VEW08_13630"
     CDS             complement(8342..9028)
                     /locus_tag="VEW08_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41828.1"
                     /translation="MKWVVIILVVAVAGAVGAYWYEGYSAESTLLGQPVYRVLKKHEP
                     AVYEKLVAEYKTYQREETSRENFINFANSEINLAATQSLAHASQNSVLALMNDMLATA
                     RQLQKAPGDTCFRYWFPLVSGPPDVARYIEPAAQARTLDLMGEVIRTAAENPSPLPEP
                     DTVKDNLAKVINETYEQFGSDAQMIAHADDPKVDRAKVCTITLSVYDRIMSLSPAESS
                     ALLRSMTQIR"
     gene            complement(9025..9552)
                     /locus_tag="VEW08_13635"
     CDS             complement(9025..9552)
                     /locus_tag="VEW08_13635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HYJ41829.1"
                     /translation="MSTKILALCGSLRSVSMNAALLRAAARLAPAGIRVEPFDEIGLL
                     PLFNPELESAMPAPVQALHAKITSSHALLIASPEYAHGVTGVLKNALDWLVSFEGFVD
                     KRVAIFNASPRSVHADAQLREILTTMSANLVADACLALPLRGTGISEQGILDSGHAAD
                     ISRALALLASGAPRL"
     gene            complement(9592..11835)
                     /locus_tag="VEW08_13640"
     CDS             complement(9592..11835)
                     /locus_tag="VEW08_13640"
                     /inference="COORDINATES: protein motif:HMM:NF025697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HYJ41830.1"
                     /translation="MTFDRADPALDTAIAISGLQSGESVVGLDVRAGGATPGELYALG
                     STGRLYTINTTTGVATLKSTLAADAGDASEPFAALAGTEFGVDFNPVADRLRVVSDTG
                     QNLRIDADTGATITDAPLTTAGTARAGVSGAAYTNAFAAACRTALYYIDATTDQLLAT
                     SDPNSGAVTVVGALGVDTTAVGDFEIATAADGTNNGYAVLVVGGVPTSYQIDLTTGVA
                     ATGGAVTRLDANELVRDTAIAPPATTPAQAAGDVYALTETGKLVSFSAAQPQKACTNT
                     TLTGQQAGESIVGIDVRPADTGLYALGSTGRLYTVDTASGALTLKSTLAPTLGDVTDP
                     FVTLVGTAFAVDFNPGNDVLRVVSDTGLNARINPDTGATTTDIATNPVGPVVTEAAYS
                     NSFVGAGTAFYYTIDTAGDGLQVLGRPSGNAINGDLQTVGALGVGDVTGVAGFDIFGT
                     NNLGLAALNIGGAATSDLFTVNLATGAATRINTVDGGEKVRGLAYGRLPVATVFALTT
                     DNHLVSFRPLTPGTFDTDVAVTGLQGAESIVGFDFRPSNGTLFAVTDAGRLYTVDPAT
                     GALSGAVTLSANTLDTTAPFTALSGTRFGVDFNPLDGQLRIQSDTGQNMRVDVDAGTV
                     VTEANLNPGTPQVVGTAFSNPYAGATSTTQYELDVAAGALVRQTSNAVLATVGSFNAG
                     TTFALEGGFDIAGGENGLPIAALQPTGAAQSTLYRVNLGTGALTSIGLLGSSGTTIVK
                     ALAIRLQ"
     gene            complement(12253..12876)
                     /locus_tag="VEW08_13645"
     CDS             complement(12253..12876)
                     /locus_tag="VEW08_13645"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HYJ41831.1"
                     /translation="MNLKQRLTSALLALALAAAAAGSASPDDPARVKPLEVGAAAPAF
                     SAREADGQPFLFQPKKLSKPTMLIFYRGGWCPYCNAHLKDLRTAVPQVSALGFEVLFL
                     STDRPEILRSSLNETVDYHLLSDNEVNAARAFGVAFRMDDATYAKYKTYGLDLEETQG
                     ATHHELPVPAVFIVDRMGNIRFAHTNADYKQRLPAADVVKAAQAAIR"
     gene            13120..14751
                     /locus_tag="VEW08_13650"
     CDS             13120..14751
                     /locus_tag="VEW08_13650"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYJ41832.1"
                     /translation="MSFETLGLKPELLRAVADKGYTVPTPIQLQAIPAVLAGSDVLAG
                     AQTGTGKTAGFTLPLLQKLGARGGRNPRALVLTPTRELAAQVAQSIQDYGKYGDLRTQ
                     VIFGGVSEKPQIDKARHGCDILVATPGRLLDLCEQRAVSLSEVQIFILDEADRMLDMG
                     FIRDIKRILKMLPQQRQNLMFSATYSDDIRQLAGGFLRKPTFLQVTPSNATADKVEQS
                     AYRVPKDHKRHLLSHLIIEGNWHQVLIFTRTKHGANRLTQQLERAGINAMAIHGNKSQ
                     NARIKALQDFKENRITALVATEVAARGLDIKELPHVVNYELPNVPEDYVHRIGRTARA
                     GSTGAAISLVSPDELSYLRDIERLLKRKIDFIEPPKFEIKEGTHEPRNATGEHQPHQD
                     PRRRPEHRRSGGGGRPNHSGGGRHGGGRNGGGNRGAGRADHAAYTQGSDAPRAAEAPR
                     SDAPRSDAQPGARAHGGGHHGGGQRSGGHGGQRAGGGGRRGGHGGQGRSGGGGGGAGR
                     SGFARGARSSGGRSGGGSSGGGSNSGSGGGFSRIT"
     gene            complement(14774..16057)
                     /locus_tag="VEW08_13655"
     CDS             complement(14774..16057)
                     /locus_tag="VEW08_13655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ41833.1"
                     /translation="MADDNKRAEDGTALIRISAALEAADSQRQDLATALAHERTLLRT
                     MIDLIPAFIYAKDVGSRFIAMNAALARNMGTAAADAIGKTDFDFFAPELAKKFYSDEQ
                     ALLKSGTAIIDLEEPGFDAVTGMPRTVCTSKVPLRDENGEVIGIIGVGFDITERKIAE
                     QRLAASEHLESIGRLAAGVAHEINTPIQYLNDSVYFIREAMQDLLAQNAKLAAALPKP
                     PEPDEDIEDLKRELPPALDRVVDGLARIAEIVRSMKEFSHVDQREMARIDLNRAINST
                     LVIARSEYKYIADLKTDFQELPLVTCHGGQVNQVVLNLVVNAAHAIADKNKAGGMAGM
                     GLITVTTRIEDGFAVISISDTGGGIPEAIRKRIFEPFFTTKEVGRGTGQGLSIAHNVI
                     KAHGGKLDFVTELGKGTTFNVRLPLGDADQESAVA"
     gene            complement(16240..16605)
                     /locus_tag="VEW08_13660"
     CDS             complement(16240..16605)
                     /locus_tag="VEW08_13660"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41834.1"
                     /translation="MKTSRTLIAIAVAALIPMGAAVAGDKDKDKAGYAGASFDTLDTN
                     RDGKVSQAEAAADTTIVFSSADANGDGYLDKSEWKNRTKGSTSPTPQSTPGPSSDPAM
                     PQGDQPASEPTPDTETPRQ"
     gene            complement(16893..19220)
                     /locus_tag="VEW08_13665"
     CDS             complement(16893..19220)
                     /locus_tag="VEW08_13665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HYJ41835.1"
                     /translation="MSAHGRTGAAPLLRSTSLLIRLRLRRLANLIAAGSQGRKKPGDK
                     TRTGNPGKRSSKIVLYLAWPMMLFMFGAITANSVMNLHGALDPPGSFWVTVEFSTALA
                     VGIAFLLFVLWFASLLVTIGSGELAKPEWDLEWLITLPVRADTLLWARLAERSIVNPS
                     GAIALIPACTAIAWFSGLRWSAPLIGVLAAWPLLLLAALVRTLIDTGLRLRMRAAQLR
                     NLHAVISVLSIITMYLAISIGLKGKSDFMLRVAGSLPDWLLYTPMGLTVRAINERTFF
                     DAGLLTLALLAETGVIVWLGLQLLRHQLRNGVVSGGARDAERGAARDAEPVADNSPAA
                     SARPALISLSAVQRRELALLRRDRNFLVQSLVLPLLIVGGQVLLGTSGVASTMWTNPN
                     VLASVAFGLAAYSLSMSAFQTLNSEGHALWLLYTFPCSIEEVLKDKAKLWGVLTLAYP
                     VVMFGIGAVVAPSLDWTFFGAMLTALLGIPIYAFIAVALGVFGSNPLEQQPNQKVKPA
                     YVYLFMTLSALYVYAILTPQPAQRLIFMVLTMLLAAALWQKARDQLGYLLDPDASPPP
                     RVSTSDGLIAAMVFFVAQAVAAAFIIGRGRVTGAAVLLAFSIGGAVTYALMRSVYAKA
                     GTEGVPQILGSTSQPLLGLAAGLAAGAVGVLYLFGLDALDLLEGAVRASKSYASLGLW
                     TLPLALIAAPLFEEFIFRGLIFGGLRRSFGIWPAALASAALFAVVHPAFSIIPVFFLG
                     ACAALVYERSRSLLAPMIVHGVYNACVIGAQTFLF"
     gene            complement(19217..19948)
                     /locus_tag="VEW08_13670"
     CDS             complement(19217..19948)
                     /locus_tag="VEW08_13670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ41836.1"
                     /translation="MIEFQQLSRTYGDFTAVHPLSLEVGSGEVFGFLGPNGAGKTTTI
                     RMMMGILVPSGGRALINGLDCHSQRAEVQRHVGYLPDNPIYYDFLRGREILQFVAEMH
                     GMGRREAGQQAVRMLAEFGLTEVVDEFAVNYSMGMKKKLGLACALIHDPQVLILDEPI
                     NGLDPRAAREVQDRLRQAAAAGKTIFMSTHLLDMAEKVCTRVGIIHRGELTATGTLDE
                     LRERVNQNVSLEEVFLQVTAEDAGS"
     gene            19984..21444
                     /locus_tag="VEW08_13675"
     CDS             19984..21444
                     /locus_tag="VEW08_13675"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HYJ41837.1"
                     /translation="MQNNKNELAPWEKADLPEPPTPRGLSWIGVCGPGVIVLGVSIGS
                     GEFLLGPATFVKHGLSLLWVTLVAVFFQTVFNTELMRYTVATGEPVVTGFMRTRPGKT
                     FWAWFYAVLYFLQIGWPAWAATAAGAIFFLFANRLATPPADSTTIYLIGIGTFLACIA
                     VLMVGKRIARTLEILNWALVACILGSFLVLGVIYVPAGTWFSAVAGFTGFDTVHGVFN
                     FIPENADVLLLGALVGYSGGGGVLNLTLSNWARDKGYGMGRRVGHIAGAVGGEKVHLA
                     STGFIFQPDTANLSRWRGWWRIVRVDQWGIFFVGALLGMMLPALLYVTFVEPGTDIKG
                     LAISAVLAQTIGATAGPWLGLCVAGLGVWILFKTQLDVVEGMTRSITDILWTGSARVR
                     GWRGGDVRAVYYTVLTVVVIWGIIALKLAAPVMLLQIGANIASAIFVLASLHVLYLNT
                     SILPVALRPPLWRRVALVGMALFYGFFVVLVARSLY"
     gene            complement(21448..>21659)
                     /locus_tag="VEW08_13680"
     CDS             complement(21448..>21659)
                     /locus_tag="VEW08_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41838.1"
                     /translation="DVWKDLYLPCFELVHPKLAMGGIIAADNMLFPESARTHADAYRQ
                     RVRAAGDMDSVLIEVGNGIEISRKR"
BASE COUNT         3739 a         6842 c         7163 g         3915 t
ORIGIN      
        1 gcgcgctggg gcaccgagct gcacgaccgc ttcatgctgc cgtacttcgt cgagcaggat
       61 ctcgaagacg tagttgccga gcagaacgcc gcgggcttcc cgttgaagac ggaatggttc
      121 gcgccgcatt tcgagttccg cttccccaag tacggcgact tcgccacgct cggcacggaa
      181 gtggagttgc gccaggcgct cgaaccctgg catgtcatgg gtgaggaagg tgcggccggc
      241 ggcgcggtgc gctacgtgga ttcgtcggtc gaaagactgc aggtgctggt gaccggtctt
      301 gcgcccgacc gctatgcgct cacctgcaat ggacgacgaa tcccgttgcg atccactggc
      361 acagtaggtg agttcgtcgc cggtgtgcgc tacaaggcct gggcgccgcc atccgcgctg
      421 catccgctaa tcccggcaca ttcaccgctg gtcttcgatc tggtcgacac ctggatgaat
      481 cgatcgatgg gtggcgcgca gtatcacgtg gcgcatccgg gcggccgcag ctacgacacc
      541 ttccccgtga acgccttcga gtccgaaagc cggcgactgg cgcgatttgt cagaatgggt
      601 cacacgccga agaaagtcac acccgtgccc gatttgccca acatcgaatt cccgttcacg
      661 ctcgatctgc gtaaagtctg atgagtgact tctgctaaag atatgcacgc ggttctcgag
      721 ccggcggacc ttgaacgata cgacgagctg aacgattcgc agggcgcgat gcgcgcccac
      781 tggcgaccgc tcatcgaccg gttgcgcgcc gacgattcgc ccgacgccgt gcgccgctcg
      841 ctcgagctca cgcgccggct cgtcgtcgag aacggcgtca cctacaacgt ctacgccgat
      901 ccccagggca ccgatcgtcc ctgggcgctg gaccctttgc ccttcgtgct gccggccgcc
      961 gaatggaaga ccatcgaagc cggcgtcgca cagcgtgcgc ggctgctgaa tgcgctgttg
     1021 tcggacctct atggcccgca gcggctgctg gccgagggac atctgcccgc cgaactgccg
     1081 ttcgggcatc ctaactacct ctggccctgc cacgacctca ggccggccga cggcacctgg
     1141 ctgcatgtct acgctgcgga tctcgcgcgc gcccccgatg gccgctggtg gctgctcgcc
     1201 gatcgcacgc aggcgccgtc gggcgccggt tatgcgctcg aaaatcgcga gatcctcgag
     1261 caggtgcatc ctgaggccat ccccgacatg gccgtccgcc gcgtccgcgg cttcttcggc
     1321 cacctgcgcg agcggatgtt ggacgccgcc ggggatgaat cgccgctcgc ggtcatactc
     1381 acgccgggcc catacaacga aacctatttc gagcacgcct atctggcgcg ccagatgggc
     1441 atgccgctcg tcgaaggcca cgatctcacg gtgcgcggcg acacggtgtt cctgaagacg
     1501 ctcggcggcc tgcgccgcgt gcactccatt cttcgccgcc tcgacgacga ctactgcgat
     1561 ccgctcgaac tgcgcggcga ctcggcgctc ggcgtcccgg ggctgatcgg cgcggtgcgc
     1621 gccgggcgcg tcatgctgtc gaacgcactc ggcaccgggg tgctcgagtc cgccgcatgg
     1681 ctcgggttcc tgccgcccat cgccgaacgg ctcatcggcg agacgttgct gctgccggag
     1741 atcgccacct ggtggctggg cgaaaagccg gcactcgaat acgtgctcgc caatctcgac
     1801 cggctggtca tcaagcctac ctacccgaac cagcgcttcg agccgatgtt cggccgcgag
     1861 ttcgagggca aggcgcgcga cctgctcatc gagcgcctgc gcaaccgtcc ctatgcctac
     1921 gtggcgcagg aacacgtgcg gctctcccag gcgccggtgt ggaagtccgc ggcgtcgccc
     1981 gaactcgcag cgcgcgcgct cacgattcgc gtgtacgcgg tggtgacgcc ggatggagtg
     2041 cgcgtgatgc ccggcggtct tgcgcgcgtg gcgaacgaag cagcggccga cgtcgtgtcc
     2101 acgcagcgcg gcggcggcgc caaggacatc tgggtgctga gcgacgatcc gcacgacgag
     2161 acgatcacgt ccgcccagtc cggaaaatgg gcgaaccttc agcgccagga ctacatcccc
     2221 tcgcgcctgg tggaaaatct ctactggctg ggtcgttatg cggtgcgatg cgagaattcc
     2281 tcgcgcctgt tgttgcgcac gctgggtgcg cgcgccgacg cgcgcgtctg gacgcacgcg
     2341 cggcagatct gccgtgacct gggtgcggtt tccgccgaag gtgagatatt cgacgcgttg
     2401 cgtacgcgcg atgcgcaagg gttgcaggct gacgtcaaac atctcgcctg gtgcgcatcg
     2461 caggtgcgca accgtctttc ggcgcgttac tggcggggtg tcgtcgggct gcagcgtcaa
     2521 atgcaggaag ccctggccac gcgcggcagc agccgtgaag ttcatgagcg ggtgctgttg
     2581 tcgctggccg cgctcacggg gttctccgaa gaagacatga tgcacgacga gggctggcgc
     2641 gtgatgcggc tcggccggcg gctcgagcgc ctgcagttcg tcgccaacat tctcgtgcgc
     2701 cagctgcaga gcgcacacgc cacgcgcccc gaggccgtcg aatggctgct cgacgtctgc
     2761 gacagcacgc ctatctaccg ggcccgttat ctcggcgtta ctcgcctctc gctgatgctg
     2821 aacctgctgc tgtatgacga tgggcaccct atggcgctgg ctttcaaccg gcggtccatc
     2881 gaccgcgacc tcgacgacct ggcgagaagc ctcgggggtg agcgcgaacg cggcgtgccc
     2941 gacgtgccgc tgctgccgca gggcgctgcc gggctgctgg atgcgcaagg caaagagggc
     3001 gatgcggcgc gtgctgctct cgccgaagaa ctcgacgacc tgtcggcgcg ctcggcggaa
     3061 ctttccgacc ggctttcgcg ccgctacttc gcgctcatcg aatccgacgc gcaggcgctc
     3121 gccacgtgag cttcgttcat gagtagaagg taccgcgtcc gccacgagac gacctacaag
     3181 tacgccgccg acgtgatgca ttcgcatcac ctgctgcacc tcgtgccgcg tcccgcgccc
     3241 taccaggaat gcctggagca tgagatccag atcgatccgg ctagccatcg ccgcgtggat
     3301 gggctggatg ctttcggcaa tccactgatc cgtctcgagc tcgcgcaacc gcatcgcgag
     3361 cttcgcgtga tctcgcaaat gcagatcgag gtgcactcaa gaccggtggt atccctggac
     3421 accaccgatt cctgggaaaa ggtccgcgat tcgtttgcct atcgcggtgc gtggccttca
     3481 cgcgaccagc tcgacgccgc gcgttttcgc catgagtcgc cgcacgtccg gttgaagcaa
     3541 tctttcaccg actactcagc ggtatgtttt ccggaagggc gcccgatcct ggtctgcgcg
     3601 gaaagcctga gcaccaagct gcacacggac atcgagtacg cgcccggcgt caccaccatc
     3661 agcacgtcgc ccaccgaagt ggtcgagaca cgccgcggcg tctgccagga cttcgcgcac
     3721 ctcatgattt cctgcctgcg ttcacgcggc ctgccggcac gctacgtgag cggttacctg
     3781 cgcaccaacg ccacgcagtc agaaagcggg gagaagctgg tgggtgatgc ggcctcgcac
     3841 gcctgggtgg cggtgtgggg tccgccctat gggtggatcg agttcgatcc caccaacggc
     3901 tgtttcgcgg gtacggatca cattgccgtg gcctggggac gcgacttcgg tgacgtatcg
     3961 ccgttgagag gcgtgatcct gggaggcgcg gatcatcagt tgtcggtgac cgtacgcgtc
     4021 gagccggaag gcgaaccgaa ctagtcggcg gccgcggcga tggccgcctt cagcttctcg
     4081 acgtccaccg attgcacgta tttgatgatg ttcttccaca tcgagtggta gccgaagcca
     4141 ccgtcggtca gctccttcag agcatcatcc ggggtccagc cctgctcgag aatccggtac
     4201 atggccgtca tgaggccggt gcggtccgcg ccatgctggc agtggatgag aaacggacca
     4261 ttttccgttt tcttcagcat gttcatgacg tcgatgacgt gtttgtcgcc gatgcgccag
     4321 gtaaggatct tcgtgtgttc ggtgcgcagc gacgttcctt cgacctcatc cttgtcgtcg
     4381 ttgaaatagc gcagattgat gacggtgcga acgcccagct tctcgatatt tttcattccg
     4441 agcgcggtcg gctgctcgct gcgatacagc gtgggtgata ttcgatgcag gttgggcacg
     4501 ccttccagtg caatgggctc ggcccagtgc gcaggtcgct ccaccacgac cgggggcgcc
     4561 tggccaccgg cttcctgcga cacgatcggg gccgcaagca cggcaaacaa acccagaagc
     4621 ggaaggcgtt tcatatggac cccgatatta gcgcaacaac gtcggccgcc gcccgtggac
     4681 gggcaacacg cttcgccgcc gcgctcatgg cggccagccg cgggcgatcc gttagcaggc
     4741 gcgagagctt gaactcgagc gtggcggcgt ccacggcctt gatggcggcg ccacattcga
     4801 gtagatagtc ggcgttgcgt tcttcctgtc cgggaatcgg cgacaccagc agcatcgggc
     4861 gctccttcgc caggcactcg gacaccgaca gcccgcccgg cttggtcacg acgaggtccg
     4921 cggcggtcat cacgcgttcg acggtggtgg tgaacccgag cggaaagagt ttccccgggt
     4981 gttgcctggc caacgcctgc aggcgcttca acaggtccgc gttgcgaccg gcgagcgcga
     5041 cgatttgcag gtcgccgggc aaccgcagga ggcgctgcgc catctcatcg agactgccga
     5101 cccccgcgcc accggccatc atcaacaccg tgaacctgtc gggcgagagg ccgagctccc
     5161 gcgcgcacac ggcgcgctcg agcggcgcgc tgaactgcgg catcacggga atcccggtga
     5221 tggaaatttt ctcgcgcggc acgccgcggt cggcgagccg gaacgccact tcttcgctcg
     5281 ccacgcagta gcggtccacg tgcgggtgca cccacaacgc gtgcacgtcg aagtcggtaa
     5341 cctgcaccca caagggtggc aggcgttttg cgctgttttt ctgcccttgt tctttcccag
     5401 gggcgcgctg gcgggacagc agctcggccg gcagaaaatg cgtgcagaga atggcgtccg
     5461 gcgcgaggcg ttcaatttcg gcggtcaatt tgcgcgtgtt gagtttctcg gcggctctct
     5521 tgagtttgcc caccagggaa tcccggctcg gtcgatcgct cttcgagtag aggaatgacc
     5581 agagctgcgg cagcttctcc accagtttga tgtactgctc gccgtagagc ttccggaaat
     5641 ccttcggcac cagcgacagc agatcgatgt gggtggccgc aaccttcgga tgggccgctt
     5701 cagcggcctt gacgatggcg tccgcggcgc gcacgtgacc ggcgcccgcg gacaccgaga
     5761 gaaccagcaa cttcattttg cggacactgc tctcaaaggc ctttgtttaa aaaccctggg
     5821 ggtgtcttgc catgctggcc gaatacaacg ccttgatctt cggcaggtcc gcggcggcgt
     5881 caccggtggg cgtgaacggc ggcatgatga cgacctggcg cttcgaatag tcgagcgcta
     5941 cgggcacgat gggcacgccg gcttccgtag cgatgtagta gaagccgagt ttccactcct
     6001 tcactttgcc gcgggtgcct tccggcgtga tggtgatcga catacgctcg cggcgcgcaa
     6061 attccgccac gcagatgcga acgatgtttc cccctttggc gcgatcgatg ggcatgccgc
     6121 cgaaccggcg cgccaggatg ccgaggggcc agaaaaagac ggtgtgcttg gccagccagg
     6181 tgttgtcgag ccgcaatacc aggaaggcac agaaaccgtg gaagaaatcc cagttggacg
     6241 tgtgcggggc gacgatcagc aggtacttgg ggacgttggg tatgttgccg gcgatgcgcc
     6301 agccggaaat ccgcatcagc aggacgccga ggtaacggcg ccagcgcgcc gggctctgcg
     6361 gagccagcgg cggaagagtg gggaaaccag aagagatgga agggtcggct gggcctgtca
     6421 tgccgcgcga tggtagccga aatggctccc tcggcgcttc gccgaaacgc gcaactctcg
     6481 gtaacggccg agaggcggac accgtttccc cgctagaggg gggatggggg aaccggtgcc
     6541 cgcgcctcgc cgtgttcgag agtgcggtcg atagttagct cctcggcgtt tttatcagct
     6601 cttcagcgga atcgaacgga acgaaacgat ttcaccagcc ggcgtcgcct tgcacgaagc
     6661 cttggcaagc agctcgttgg tcctggaatg gcgcgcgatc accgtgaagg cgttcgggtc
     6721 gaccatgtcc gagacgagcc tgctgggcgc cttgaccttg tacgccggca gcgtttcggt
     6781 cttcgcgatg gtctcgatga gcgccttgct gcacgcggcc agcgcgggcg tcacggcggc
     6841 ggtgccggca ttcccgtagg aagcgaagcc gatcatcgcg gacaagacca gatactttga
     6901 ggttcgacga atcatgttgc actcctgata atgtttgaaa tccacgagtg tcaaactacc
     6961 cgctcaggcc tgcttcaaca aacgagaaga ttcggcgcga gcatcagttt tgttcatcat
     7021 cgactggctc atcggtgcgg cgagctccat gagccacgcg cacaaagcgc gaatgtcgtc
     7081 acggttttcg tcctcggtac ggactaataa ggtgtactcc tcgtgcgtca cgagttcgtc
     7141 atcgaacacg cgtaccaggc ggccgctgtc gaacttgcgg cgcgagagct cggcgggcaa
     7201 cagcgcgata ccgatgctct tctccgcggc gcgcgtcgcc gccgacatcg agtcgatgcg
     7261 aacgatctgc gcgggctgca ggtttttcat gccgacggcg gtcgcccagc gatcccagtc
     7321 gtcacggcgc gcctcgtgca ccagcagcgt gtgcaggttc agatcctcgt agcgaagcac
     7381 gggacgctcc aggagcagct cgggcgagca ggcggggacc atcgtctgcg cgaacagcgg
     7441 atgggcgatc agtgagccgc cccacggtcc cgagccaacc accaccgaga tgtcggcttc
     7501 ggcggcgtga gtacggccgt gcacgccctg cgtttcgatg cgcaggtcga tgccgtcgtg
     7561 cagcttcgag aattccgaaa gccgcggcag cagcatctcg ctcgcgaaat atgccggcac
     7621 gtgcagccgc acgctgttgc gcccgtagcg cgcacgcagc tgttcggtga cggattccag
     7681 acgcatgaac agatcgtcta tctgctcgag atagcgcgca ccggcatcgg tgagcgccag
     7741 cgcccgcgca ctacgcgaaa acagcggcag gccgagctgc gtttccagcg tcttgatctg
     7801 atgactcacc gccgacggcg tgatgcagag ctcatcggct gcgacggcga agctcgaatg
     7861 tttggcggcg atctgaaagg tcttgatggc cttcagtgag ggagtacgca ttccattgga
     7921 tcacccaaga actcagcaaa aaagctgcga tgccaggagt aaagcaaggt gcgtgccagc
     7981 tcgggaaaag acgtaagtgc atgttcttat tcgccctgca cctttgacgg gctgtcagct
     8041 ttgttcatcc gtggcgcgca attcccgcgg ttgcaccggg tccgggtggg tgccgggtgg
     8101 ggattgtggg gagaaggatt tgtcacatgt ccgtcaaggt tctgtgcgcc ttctccccac
     8161 aatccccacc cggcaccatg actcctcggc ccctttctcc ttcttgattc ggccgccatg
     8221 ggtggctcac gcgcgggctt ccagaatttc actatgggca acgagctaaa tgttcaccgc
     8281 gccgtgctcg ccggcgtggt ttaccttgag gatgcgatcg gcgtgagtca gcgcgacagg
     8341 cctagcggat ctgcgtcatc gagcgaagca gcgcgctgct ttccgcgggt gacaggctca
     8401 tgatgcggtc gtataccgac agggtgatgg tgcacacctt ggcgcgatcg actttcgggt
     8461 cgtcggcatg cgcgatcatc tgtgcgtcgg agccgaactg ctcgtaggtc tcgttgatca
     8521 ccttcgcgag attgtctttc accgtatcgg gttccggcag ggggctgggg ttttcggcgg
     8581 cggtgcggat cacctcgccc atgaggtcga gcgtgcgggc ctgcgccgca ggctcgatgt
     8641 atctggcgac atccggtggc ccggagacca gcgggaacca gtatcggaag caggtatctc
     8701 cgggggcttt ctgcaattgg cgggcagtgg ccagcatgtc gttcatgagc gcgagcactg
     8761 aattctgcga ggcgtgcgcc aggctctgcg tggccgcgag gttgatctcg gaattggcga
     8821 agttgatgaa attctcgcgg ctggtttctt cgcgctgata ggtcttgtac tccgccacca
     8881 gcttctcgta caccgccggt tcgtgcttct tgagaacgcg gtacacgggt tgcccgagca
     8941 gcgtcgactc ggcgctataa ccctcgtacc agtacgcgcc cacggcgccg gccaccgcca
     9001 cgaccagaat gatgactacc catttcataa acgcggtgct cccgacgcca gcaaggcgag
     9061 cgcccggcta atatccgcgg cgtgccctga gtcgagaata ccctgttccg agatgccggt
     9121 gccgcggagt ggcagggcga ggcacgcgtc ggcgaccagg ttggccgaca tggtagttag
     9181 tatctcgcgc agctgggcgt cggcgtgcac cgaacgtggc gaggcattga agatggcgac
     9241 ccgtttgtcg acgaatccct cgaagctcac cagccagtcg agcgcgttct tgagtacgcc
     9301 ggtcacacca tgtgcgtatt ccggactggc gatgagcagg gcgtgactcg aggtgatttt
     9361 cgcgtgcagt gcctgcaccg gagccggcat cgctgattcg agctccgggt tgaacaatgg
     9421 cagcagtccg atttcgtcga acggctcgac gcggattccg gcaggagcaa gccgcgccgc
     9481 ggcgcgcagc agcgcggcgt tcatcgagac gctgcgcagg ctgccgcaca gcgcgaggat
     9541 tttcgtcgac atggggcggc agcctagcag ccgccgcccc gtgccacacc cttactgaag
     9601 gcggatcgcg agcgccttga cgatggtcgt gccgctcgat cctaacaagc cgatcgaggt
     9661 cagcgcgcca gtgcccaggt tcacgcggta cagcgtcgac tgcgcggcgc cggtcggctg
     9721 cagcgcggca atcggcagtc cgttttcgcc gccggcgatg tcgaagccac cttcgagcgc
     9781 aaacgtcgtg ccggcgttga acgaaccgac cgtggccagc acggcgttcg aggtctgtcg
     9841 tacgagtgca ccagccgcca cgtcgagttc gtactgcgtg gtgctggtcg cgcccgcata
     9901 cggattcgag aaggcggtgc ccacaacctg aggcgtaccc ggattcagat tcgcttcggt
     9961 gacgacggtc ccggcatcca cgtccacgcg catgttctgg cccgtgtcgc tctgaatgcg
    10021 cagctgaccg tcgagcggat tgaagtccac gccgaatcgc gtgcccgaca gcgcggtgaa
    10081 tggcgccgtt gtgtccagcg tattggcgct cagggtcaca gcccccgaca acgcaccggt
    10141 cgccgggtcg accgtgtaca gtcgccccgc gtccgtaacc gcaaacaacg tgccattcga
    10201 cggacggaag tcgaagccga cgatcgactc ggcgccctga agtcccgtga ccgcgacgtc
    10261 ggtgtcgaac gtgcctggag tcagcggcct gaagctcacg agatggttgt cggtggtcag
    10321 cgcgaacacc gtggcaaccg gcaatcgtcc gtacgcgagg ccgcgcactt tttcgccgcc
    10381 atccacggtg ttgatgcgcg tcgccgcgcc cgtggccagg ttcacggtga acagatccga
    10441 agtcgccgcg ccaccgatgt tgagcgcagc caggccaagg ttgttagtgc cgaagatgtc
    10501 gaacccggcc acaccggtca cgtcacccac gcccaacgcg ccgaccgtct gcagatcgcc
    10561 gttgatggcg ttgcccgacg ggcggcccag gacctggagg ccatcgcccg ccgtatcgat
    10621 ggtgtagtag aacgccgtgc cggcgcccac gaagctgttg ctgtacgccg cctcggtgac
    10681 caccggaccc accgggttgg tcgcgatgtc ggtcgtggtt gcgccggtgt cggggttgat
    10741 gcgtgcgttc aggcccgtgt cgctgaccac gcgcaacacg tcattgccgg ggttgaagtc
    10801 caccgcaaac gctgttccga cgagagttac gaatgggtcc gtgacatcgc cgagcgtcgg
    10861 tgcgagcgtc gacttcaacg tgagcgcgcc gctggccgta tccaccgtgt acaggcggcc
    10921 ggtgctgccg agcgcataca gcccggtgtc ggcgggacgc acgtcgatac cgacgatgct
    10981 ttcgccggct tgctgaccgg tgagcgtcgt gttcgtgcac gccttttgcg gctgggccgc
    11041 gctgaacgac accagcttgc cggtctcggt gagtgcgtac acatcgccgg cggcctgcgc
    11101 cggtgtggtg gcaggcggcg cgatggccgt gtcgcgcacc agttcgttgg catcgaggcg
    11161 cgtcactgcg cccccggtgg ccgcaacgcc ggtagtgagg tcgatttggt acgaggtcgg
    11221 tactccacca accacgagca ccgcgtagcc gttgttcgtg ccgtcggctg cggtcgcgat
    11281 ttcgaagtcg cccaccgcgg tcgtatcgac accgagcgcg ccgaccacgg tgaccgcacc
    11341 ggaattcgga tcgctggtgg ccagcagttg atcggtggtc gcatcgatgt aatacagagc
    11401 cgttcggcag gccgcggcga atgcgttggt atacgccgcg ccactcacgc ctgcgcgcgc
    11461 ggtgcccgcg gttgtgagcg gggcatcggt aatcgtcgcg ccggtgtcgg cgtcgatccg
    11521 caagttctgt ccggtatcgc tgaccacacg cagacgatcc gcgaccggat tgaagtcgac
    11581 gccgaattcc gtcccggcga gtgccgcgaa gggttcgctg gcgtcgccgg catcggcggc
    11641 cagagtcgac ttgagcgttg caactccagt ggtggtgttg atcgtgtaga ggcggcccgt
    11701 gctgccgagg gcatacaact cgccgggtgt cgcgccgccg gcgcgtacgt cgagtccgac
    11761 taccgactcg ccggattgaa gacctgagat ggctatcgcc gtgtccaacg cgggatcggc
    11821 gcggtcgaaa gtcacgaggc ggcccgtgct ggtgattccg taggtgtcgc ctgacgtgac
    11881 gcctgcaccg ggtgcgggta cggcaggagg cggaggcggc ggcggcggag gcggaggcgg
    11941 aggcggcggg ggcggcgggg gtggaggtac tgtcggagga ggacttggcg attggggcgg
    12001 tggattgtcg ccacctccgc cgccgcacgc gctgagagcc gccacaatga gaccggtcgc
    12061 gagcaagccg cgagcaacgc catgcgtagt cataaattcc ctcggtgtga aagattccat
    12121 tcaccccgag agtacgaact ggcgacgtct gcgaatgccg acattcgcag cgacctgagt
    12181 gccaaaagcg atccgtacgc agtcggcccg cgcctacgaa agcacgaccc gctgacacgc
    12241 ttcggtaact aactagcgga tcgcggcctg cgccgccttc acgacgtccg ccgccggcag
    12301 ccgctgcttg tagtcggcat tggtgtgcgc gaaacggata ttccccatgc ggtcgacgat
    12361 gaataccgcg ggaaccggca gctcgtgatg ggtcgcgccc tgggtttcct cgagatcgag
    12421 gccgtaggtc ttgtatttgg cgtaggtggc atcgtccatg cggaaggcga cgccgaaggc
    12481 gcgggcggca ttcacttcgt tgtcggacaa caggtggtag tcgacggtct cattgagact
    12541 cgatcgcagg atctcggggc gatcggtgct gaggaacagc acttcgaacc caagcgcgga
    12601 gacttgcgga accgcggtgc gcaggtcttt gagatgcgca ttgcagtagg gacaccagcc
    12661 accgcgatag aagatgagca tggtgggttt cgaaagcttt ttcggctgga acaggaacgg
    12721 ctggccatcg gcttcccgcg ccgagaacgc gggggcggcc gccccgactt cgagtggttt
    12781 gaccctggcc gggtcgtcgg gagatgcact gcccgcagcc gcggccgcga gagccagggc
    12841 caggagcgcg gatgtgagtc tttgtttgag gttcatgagc cggagaccgg cagtctggcc
    12901 gtcttatttc agccctttgt gaggccctcg ctggaataca ggggggtgcc catgtatagt
    12961 cgcgcccgct tatccagggt cgcgcggtca tcgcccccta aagcacgcaa gtcgacttac
    13021 tacgattcgc aactcctctc ctcgctaatg accgcttcaa gcgccttgtg cgttatgaac
    13081 tgcgagtctt tcaagcttcc tttcaaggta ttcaactaaa tgtcatttga aactctcggg
    13141 ctcaagcccg agttgctccg tgccgtggcc gacaagggtt atacggttcc cactcccatt
    13201 cagcttcagg ccatcccggc agtcctcgcc ggcagcgacg tgctcgcggg cgcgcagacc
    13261 ggcactggca agacggccgg cttcacgctg ccgcttctgc aaaaactcgg cgcccgcggc
    13321 ggccgcaatc cgcgcgcgct ggtgctgacg cccacgcgtg agctcgcggc ccaggttgcg
    13381 cagagcatcc aggactacgg caagtacggc gatctgcgca cgcaggtcat cttcggcggc
    13441 gtctccgaga agccgcagat cgacaaggcg cgccatggct gcgacatcct cgtcgcgact
    13501 cccggccgcc tgctcgacct ttgcgaacag cgcgccgtgt cgctctccga agtgcagatc
    13561 ttcattctcg atgaagcgga ccgcatgctc gacatgggct tcatccgcga catcaagcgc
    13621 atcctcaaga tgttgcctca gcagcgtcag aacctcatgt tctcggcgac ctactcggac
    13681 gacatccggc agctcgccgg cggcttcctg cgcaaaccca ccttcctcca ggtaactccc
    13741 agcaacgcca ctgccgacaa ggtcgagcag agtgcgtacc gcgtcccgaa agatcacaag
    13801 cgccacctgc tctcgcacct gatcatcgag ggcaactggc accaggtgct gatcttcacg
    13861 cgcaccaagc acggcgccaa ccgtctcacg cagcagctcg aacgcgctgg catcaacgcc
    13921 atggccatcc atggcaacaa gagccagaac gcgcgcatca aggcactgca ggatttcaag
    13981 gaaaaccgca tcacggcgct ggtggcgacc gaagtcgccg cgcgcggcct cgacatcaag
    14041 gagctgccgc acgtcgtgaa ctacgaattg ccgaacgtgc cggaagacta cgtgcatcgc
    14101 atcggccgta cggcgcgcgc cggttcgacc ggcgccgcga tctcgctggt ttcgccggat
    14161 gagctcagtt acctgcgcga catcgagcgg ctgctcaagc gcaagatcga tttcatcgaa
    14221 ccgccgaagt tcgagatcaa ggagggcacg cacgagccac gcaacgcgac cggcgaacac
    14281 cagccgcacc aggatccgcg tcgtcgtccg gaacatcgcc ggagcggcgg tggcggccgg
    14341 cctaaccact caggtggtgg acggcatggc ggtggacgca atggtggcgg aaatcgcggc
    14401 gcgggacgtg ccgatcacgc ggcgtacacg cagggcagcg atgctccgcg tgccgccgaa
    14461 gctccgcgtt cagatgctcc gcgcagcgac gcccagcccg gtgctcgtgc gcacggcggt
    14521 ggtcatcacg gtggcggcca gcgtagcggc ggtcatggtg gtcaacgcgc gggtggcggt
    14581 ggacgccgcg gtggtcacgg tgggcaaggt cgcagcggcg gtggtggcgg tggcgccggt
    14641 cgcagcggtt tcgcacgcgg tgcgcgctcg agtggtggcc gcagtggcgg tggctccagt
    14701 ggcggcggaa gcaattccgg cagcggcggc ggtttctcgc ggattacctg attcgggcct
    14761 gatccgggtg cggtcaggcg acggctgact cctgatcggc gtcaccgagc ggcaggcgca
    14821 cgttgaacgt cgtgcccttg ccgagctcgg tcacgaaatc cagcttgccg ccgtgagcct
    14881 tgatcacgtt gtgggcgatc gacaggccct ggccggtgcc gcgacccact tctttcgtcg
    14941 tgaagaacgg ctcgaagatg cgcttgcgga tcgcttcggg aatgccgcca ccggtatcgc
    15001 tgatgctgat gacggcaaat ccgtcttcga ttcgcgtcgt gacggtgatc aaacccatcc
    15061 cggccatgcc cccggccttg ttcttgtcgg caatggcatg cgccgcattg accacgaggt
    15121 tcaacaccac ctggttgacc tgtccgccat ggcaggtcac caacggcagc tcctggaaat
    15181 ccgttttcag gtcggcgatg tacttgtact cgctgcgcgc gatcacgagc gtgctgttga
    15241 tggcgcggtt caggtcgatg cgcgccattt cacgctgatc gacgtgtgag aactctttca
    15301 tcgagcgcac gatttccgcg atgcgcgcca ggccatcgac cacgcggtcg agcgccggcg
    15361 gcaactcgcg cttgagatcc tcgatgtctt cgtccggttc gggcggtttc ggcagcgccg
    15421 cggcgagttt cgcgttttgc gcgagcagat cctgcatggc ctcgcgaatg aaatagacac
    15481 tgtcgttcag gtactggata ggcgtattga tttcgtgggc gacgccggcg gccagccggc
    15541 cgatggattc gagatgctca ctcgccgcca gccgctgttc ggcgatcttg cgttcggtga
    15601 tgtcgaagcc aacgccgatg atgccaatca cctcgccatt ttcatcgcgc agcgggacct
    15661 tcgaagtgca gacagtgcgg ggcatgcccg tgacggcatc gaaaccaggc tcttcgagat
    15721 cgatgatggc agtgccggat ttgagcaggg cctgttcgtc ggagtagaat tttttcgcga
    15781 gctccggagc gaagaagtcg aagtcggttt tgccgatcgc gtccgcggcc gcggtgccca
    15841 tgttgcgtgc gagcgcggca ttcatggcga tgaagcggct gccaacgtcc ttggcataga
    15901 tgaacgcggg gatcaggtcg atcatcgtgc gcagcagcgt gcgctcgtgc gccagcgcgg
    15961 tggccaggtc ctggcgctgc gagtcggcag cttcgagggc ggcgctgatg cggatcagcg
    16021 cggtgccgtc ttctgcacgt ttattatcat cagccaccgg gaactccaat tgactccaac
    16081 ccggcgtaac ggccgatccg ggttgaaact gaagtgcgag gcggggttgt gagacggtcg
    16141 tcacgtcccc taagtcattc ccggcccaaa aaagagagat ccgcgcgacc cggtgggcgt
    16201 tgcgcggatc tggccgacgc ggcccgaggc aaaggaaaat tattgccgcg gagtttccgt
    16261 atcgggcgtc ggctcgctgg cgggctggtc accctggggc atggccggat ctgacgacgg
    16321 tcccggagtc gactgcggag tggggctggt cgagcccttc gtgcgattct tccattccga
    16381 cttgtcgaga taaccatcgc cgttggcatc ggcgctcgag aacacgatgg tcgtgtccgc
    16441 ggcggcctcg gcctgcgaaa ccttgccgtc ccggttggtg tcgagcgtat cgaagctcgc
    16501 gccagcgtaa ccagctttgt ccttgtcctt gtcgcctgca acggccgcgc ccatcgggat
    16561 cagcgccgcg accgcaatgg cgatcaatgt tctggatgtc ttcatgattt ctccttgagc
    16621 tcaatggaag ggttgagcgg cttcgtcgcc cgaaggatga attcggcgtc gtgccacact
    16681 tcacttgggc agcaatcgac gtgccaggtt caatctccgc agcgggcgca tgcatcgccg
    16741 atttcgtgcg cgccaggtcg ccgcgccgcg acaatcgcca ttgcgggcga cgtcgatccg
    16801 cgacgcaaaa cgcgaaccta actacaaaca atcacttgcg agtcggcgtg cggggacgtc
    16861 ggacaaaacc cgacacccgg cgcgtatttc gctcagaaca ggaatgtctg cgcgccgatc
    16921 acgcaggcgt tgtacacgcc gtgcacgatc atcggcgcca gcaggctgcg gctgcgttca
    16981 tagaccagcg ccgcgcaggc gccgaggaag aaaaccggga tgatcgaaaa tgccgggtgc
    17041 acgacggcga acagggcggc gctcgcgagc gcggcgggcc atattccgaa gctgcgccgc
    17101 aggccgccga agatgagccc gcgaaaaatg aattcctcga acagcggcgc ggcgatgagc
    17161 gccagcggca gtgtccacaa tccgagcgag gcgtacgact tgctggcgcg cacggcaccc
    17221 tcgagcagat ccagcgcatc gagcccgaat agatagagca cgcccaccgc cccggcggcg
    17281 agccccgcgg cgaggccgag cagcggctgg ctggtgctgc cgagaatctg cggcacaccc
    17341 tcggttccgg ccttggcata caccgaacgc atgagtgcgt aggtgactgc gccgccaatg
    17401 gagaacgcga gcagcaccgc cgcgccggta acccggccgc ggccgatgat gaaggcggcc
    17461 gccaccgcct gcgcgacgaa gaacaccatg gccgcgatca ggccgtcgga cgtcgagacg
    17521 cgtggcggcg gcgaggcatc cgggtcgagc agatagccca gctgatcgcg cgccttctgc
    17581 cacagcgcgg ccgccagcag catcgtcagc accatgaaga tgagtcgctg cgccggctgc
    17641 ggtgtgagga tggcgtagac gtacagcgcg gacaacgtca tgaagaggta gacgtaggcc
    17701 ggcttcacct tctggttcgg ctgctgctcg agcgggttgc tgccgaaaac gccgagtgcc
    17761 acggcgatga atgcgtagat ggggatgccg agcagcgccg tcagcatggc gccgaaaaac
    17821 gtccagtcga gtgacggcgc aaccacggca ccgatgccga acatcacgac tggataggcg
    17881 agcgtgagga cgccccagag cttggccttg tccttgagga cttcttcgat ggagcacggg
    17941 aacgtgtaca gcagccacaa tgcatggccc tcgctgttca gcgtctggaa cgccgacatg
    18001 ctgagtgaat aggccgcgag accgaaggcc acggaggcga gcacgttggg attcgtccac
    18061 atggtgctgg caacgcccga agtgccgagc agcacttgtc cgcccacgat gagcaacggc
    18121 agcaccaggc tctgcacgag gaaattgcgg tcgcgacgca gcagcgcgag ctcgcgccgc
    18181 tgcacggcgc tcagggaaat gagcgccggt cgcgctgatg ccgcggggct gttgtcagcg
    18241 acgggttcgg catcccgggc ggcaccgcgc tcggcatcac gggcgccgcc ggacaccaca
    18301 ccgtttctga gctgatgccg cagcagttgc aggccgagcc agacgatgac gccagtctcg
    18361 gcgagcagcg cgagggtgag caggcccgca tcgaagaacg tgcgttcatt gatggcgcgc
    18421 acggtcagac ccatcggcgt gtacagcagc cagtcgggca gcgagcccgc gacacgcagc
    18481 atgaaatccg acttgccctt gaggccgatg gagatggcga gatacatcgt gatgatggag
    18541 agcacggaaa tgactgcgtg cagattgcgc agctgggctg cccgcatacg gaggcgcagg
    18601 cccgtgtcga tcagcgtgcg caccagcgcc gcgagcagca gcagcggcca cgccgccagc
    18661 acgccgatga gtggcgcgga ccagcgaagt ccggaaaacc aggcgatggc ggtgcatgcc
    18721 ggaatcagcg cgatcgcccc ggagggattg acgatgctgc gctcggcgag gcgggcccac
    18781 agcagcgtat cggcacggac cgggagcgtg atgagccact cgagatccca ttcgggtttg
    18841 gcgagttcgc cggaaccgat cgtgaccagc agcgatgcaa accacaacac gaacagcagg
    18901 aacgcgatgc cgacggcgag ggcggtgctg aattcgacgg tgacccagaa actgcccggt
    18961 ggatcgagcg cgccgtggag attcatcacc gagttggccg tgatcgcgcc gaacatgaac
    19021 agcatcatcg gccaggcgag gtagaggacg atcttgctgc tccttttccc cggattgccg
    19081 gtgcgtgtct tgtcgcccgg cttctttctg ccttgcgacc cggcggcgat caggttggcc
    19141 agccggcgca gccgcaaccg gatcagcagc gaggtgctgc gcaacagcgg cgcggcaccg
    19201 gtgcggccgt gtgcgctcac gagcccgcgt cttcggcggt gacctgcagg aacacttctt
    19261 cgagcgagac gttctgattc acgcgctcgc gcaactcgtc cagcgtaccg gtggccgtga
    19321 gctcgccgcg atggatgatg ccgacgcgcg tgcagacctt ttccgccatg tcgagcaggt
    19381 gcgtggacat gaagatggtc ttgcccgccg ccgccgcctg ccgcagccgg tcctgcacct
    19441 cgcgcgccgc acgcgggtcc agcccgttga tgggctcgtc gaggatcagc acctgcggat
    19501 cgtggatcag cgcacaggcc agccccagct tctttttcat gcccatcgag tagttgaccg
    19561 cgaattcgtc caccacttcg gtgaggccaa actcggcgag catgcgcact gcctgctgcc
    19621 cggcttcgcg ccgtcccatg ccgtgcattt cggcgacgaa ctgcaggatc tcgcggccgc
    19681 gcaggaagtc gtagtagatg ggattgtcgg gtagatagcc gacgtggcgc tgcacttcag
    19741 cccgctgcga atggcaatcg agcccgttga tcagcgcgcg gccgccgctc ggcaccagga
    19801 tccccatcat catgcggatt gtcgtggtct tgccggcgcc gttgggaccg agaaagccga
    19861 agacctcgcc gctcccgact tcgagagaga ggggatgcac ggcggtgaaa tcgccgtagg
    19921 ttcgcgaaag ctgctggaat tcgatcatgc gcgtcagtgt atgctgaggt gcaggagggg
    19981 tcgatgcaga acaacaagaa tgaattggcg ccctgggaga aggcagatct cccggagcct
    20041 cccacgccgc gcgggttgtc gtggatcggc gtttgcgggc cgggtgtcat cgtgctcggc
    20101 gtctcgatcg gcagcggcga gttcctgtta ggccccgcca ccttcgtcaa acatggtctg
    20161 tcgttgctgt gggtgacgct ggtcgcggtg ttcttccaga ccgtgttcaa caccgagctc
    20221 atgcggtaca cggtcgccac gggcgagccc gtggtaaccg gcttcatgcg cacgcggccg
    20281 ggcaagacct tctgggcgtg gttttacgcg gtgctctatt ttctgcagat cggctggccg
    20341 gcgtgggccg cgaccgctgc cggcgccatc ttcttcctgt tcgccaacag gctcgcgacg
    20401 ccgccggcgg attcgaccac tatctacctg atcggcatcg gcacgttcct cgcatgcatc
    20461 gccgtgctga tggtcggaaa acgcatcgcc cgcacgctcg agatcctcaa ctgggccctg
    20521 gtcgcgtgca ttctcggcag cttcctggtc ctgggcgtca tctacgtgcc agcagggacc
    20581 tggttttcgg ccgtggccgg cttcacgggc ttcgacaccg tgcacggtgt attcaacttc
    20641 ataccggaaa atgccgacgt cctgctgctg ggcgcgctgg tgggctattc gggtggcggc
    20701 ggcgtactca acctcacgtt gtcgaactgg gcgcgcgaca agggttatgg catgggccgt
    20761 cgcgtggggc acatcgcggg cgccgtcggc ggcgaaaaag tgcacctcgc ctcgaccggg
    20821 ttcattttcc agcccgatac cgcaaacctt tcgcgatggc gcggctggtg gcgcatcgtg
    20881 cgcgtcgacc agtggggcat cttcttcgtc ggagccttgt taggcatgat gctgccggcg
    20941 ttgctgtacg tgaccttcgt cgagcctggc accgacatca agggactcgc catcagcgcc
    21001 gtgctcgccc agaccatcgg cgccaccgcc ggcccatggc tggggttgtg cgtcgccggg
    21061 ctcggcgtat ggatcctgtt caagacgcag ctcgatgtcg tggaaggcat gactcgttcc
    21121 atcaccgaca ttctctggac cggcagcgca cgcgttcgtg gctggcgcgg cggcgatgtg
    21181 cgagcggtgt attacacggt gctgaccgtc gtggtgatct ggggaatcat cgcgctgaaa
    21241 ctcgcggcgc cagtaatgct cctgcagatt ggcgcgaaca tcgccagtgc gatcttcgtg
    21301 ttagcgtcac tgcacgtgct gtatctcaac acgagcatcc tgccggttgc gctgcggccg
    21361 ccgctgtggc gccgcgtggc gctcgtcggc atggcgctgt tctacggatt cttcgtggtg
    21421 ctggtggccc ggtcgctgta ctgaatatca gcgcttgcgg ctgatctcga tgccgttgcc
    21481 gacttcgatc agcaccgaat ccatgtctcc cgcggcgcgc acgcgctggc gataggcatc
    21541 ggcgtgcgtg cgcgcgctct cggggaacag catgttgtcg gcggcgatga tgccgcccat
    21601 ggcgagcttg ggatgcacga gttcaaagca ggggaggtaa agatccttcc agacgtcga
//
LOCUS       DASQJS010000343         6624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3665698, whole genome shotgun sequence.
ACCESSION   DASQJS010000343 DASQJS010000000
VERSION     DASQJS010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3665698"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VEW08_13685"
     CDS             <1..1091
                     /locus_tag="VEW08_13685"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HYJ41839.1"
                     /translation="DNVVPLRSFNDNRFIFEEVETVADGLGPTFNAQACRECHQNIVT
                     GGASQVAEHRTGRLELLQFVESIGGSLIQSRSTNAEIFERVPFEDSVSTFRISTNLLG
                     GGFVEAVANSTLLSIRDRQPAAIRGTALEVAVLEANNAPRIGRFGWKSQHASMESFAA
                     DAYLNEMGITTPVLPEENTSLGRNVGFPSPFDPVPDPEDDGIDVVAFANFMRATKAPP
                     RGPINSTVRAGETLFNSVGCGGCHIPTIRTAAPGTRINGGAIRVRDAVGNKIIHPYSD
                     FLLHDIGTGDGIPVLPGPAFASTATQIRTAPLWGLRTRNRLMHDGLSFTKQEAISRHA
                     GQAAGVRAAYDALTTAQKDQVLAFLDSL"
     gene            complement(1158..1553)
                     /locus_tag="VEW08_13690"
     CDS             complement(1158..1553)
                     /locus_tag="VEW08_13690"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ41840.1"
                     /translation="MKIVINIDVPELHAAIAFYTAALGLTHSRTLDDDVAELTGASAT
                     LYLLQKKSGTAAVKAPPVERGYGRHWTPVHFDLVVPDVDAAAARAIAAGAKQETGHVD
                     WRGSRCISFGDPFGHGFCFIQFEHGTYAG"
     gene            complement(1550..2398)
                     /locus_tag="VEW08_13695"
     CDS             complement(1550..2398)
                     /locus_tag="VEW08_13695"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYJ41841.1"
                     /translation="MFRIGDFSRIARVSARLLRFYDELGLFVPAHADEQTGYRYYAIE
                     QLAELNRILVLKDLGFNLDQVRDILRAKIAPSELRNMLVLRRNDVEQTLALEARRLQQ
                     IETRIAQLESEGTLSADDVIVRAEPAREILSLRRTVPSFAAARGLILELRELARPLLP
                     RGTTPTLLAIAHSPQFEQDELDVEFAYVLDGMPPVAGRAGHSLTRRTLEGVERMAVCV
                     RVGLPEDAHLVTAKIGQYLAASGETLAGPSREVFLKLPTPERMHESVVEMQFAIGPPA
                     APGAPA"
     gene            complement(2382..3065)
                     /locus_tag="VEW08_13700"
     CDS             complement(2382..3065)
                     /locus_tag="VEW08_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYJ41842.1"
                     /translation="MLKIYGVPISVHTRKVIVVALAKGLEFEVIPVVPVIPGNPPANW
                     RELSPTGKIPALTDGDFTVADSAAICAYLEKIHPAQPVYPSAPREYATALSFEQYAGA
                     LFRDVVQPLFQETFVFPRIQQVATNQKRVDEVQAEVVPEMFGFLDAAIRGDYLAGDRP
                     TVADFAVVSNLINYQYIGFELPRGRFRRLAALFDRVMRHPPMVEAMRREQPVVDSMAL
                     NRSWNVQDR"
     gene            complement(3127..5151)
                     /locus_tag="VEW08_13705"
     CDS             complement(3127..5151)
                     /locus_tag="VEW08_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41843.1"
                     /translation="MREAFGASSDGYKVTLKISPKGLAPEAFTISPRGKTIAITGGDA
                     RGLIYGALALREQLLNGTPIDQVTASREKPVLPFRGIKFNTPWDTYRPSSALDQHYAT
                     ARDLKFWEAWLDMMVENRFNAFTLWTMHPFTYMVRPINFPEASKWSDTEFAEWQRLYR
                     EIFRMAKERGLDTYVVFWSIFVSEEFAKANDVAKENFYPHYYVPGDTSDITKRYLRES
                     VTQMLEEYPDLDGIGVSHGEGMGGMTPLQRQQFVDEVYVAGALNTKRKQPVKLIHRVP
                     FSSGLSSGPGVSTEVEQITRTAMEKLGHKFAGPIWAEMKFNWSHGHSTPKLVKVHGGK
                     LGDTYFKPTPSNYKIVWQVRNEDFFALRWGVPDFIRQHIALNGTQDYVGGYFIGSETY
                     IPALDYFTAGTHTALKEPVDWKWAFQRQWLFYKLWGRLLYDPKTPDAQFHAEFTRRYG
                     KRGENLLKAYSLASNTQMRLASLYDSRWDFTLYGEGMLALQGEHTNYIGVDALIDQPT
                     LDPTYVSVAEYVKTMRAGGTFGADRITPLQLADVLERDCREATRLVNLIEAADNASLR
                     YEVADVQAWSYLGVHLAEKLRGAVALANYRIAGDEKIKQQAIGHLQQALGFWTQLSTL
                     TRPLYRDMKLTHYNGNSFDANPDNLFHWERIRAEVAHDVEVAVAASPPAK"
     gene            5500..>6624
                     /locus_tag="VEW08_13710"
     CDS             5500..>6624
                     /locus_tag="VEW08_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HYJ41844.1"
                     /translation="MSLRRPSAVKWRRAAAFIALLSTTIFAFLSPSAAMAQTLPTAPT
                     VASQMTVGWNLGNTLEAICSETAWGNPAASQTLINAVKAAGFNSIRIPVSWDCHTAPS
                     GTTTIPADWMARVKQVVDYAISQDMFVIINIHWDNGWLQDHPVFASQTAINAKQQAYW
                     TQIATTFRTYDHHLLFAGTNEVHADYGTPTAEHNTVQQSFNQTFVNAVRATGGNNASR
                     SLVVQTYNTNIQHGFNFFNMPTDTIASRLMVEVHNYDPYDFTLNPNGACNVWGSRFPD
                     SGSNCNWAYESYFDSLFAQVKAKWVDQGIPVILGEYGVGTRPGKNLEARAYWNEYINR
                     AAAANGVKTFYWDNGAQPGSNDAFAIFNRSTGAIVDQAVLD"
BASE COUNT         1261 a         2012 c         2105 g         1246 t
ORIGIN      
        1 aggacaacgt cgtgccgctt cgttcattca acgacaaccg cttcatcttt gaggaagtcg
       61 agacggtcgc tgacggacta ggaccaacat tcaacgccca ggcgtgccgt gagtgtcacc
      121 agaacatcgt gaccggcggc gcgagccagg ttgccgagca tcgaaccggc cgcctcgaac
      181 tgctgcagtt cgtggagtcg atcggcggct cgctgatcca gtcgcgttcc accaatgcgg
      241 aaatcttcga gcgagtaccg ttcgaagaca gcgtgagtac cttccgcatt tccaccaacc
      301 tgcttggcgg tggattcgtc gaggcagtgg caaattcgac gctgctctcg attcgtgacc
      361 ggcaacccgc ggcgattcgt ggcacggcgc tggaggttgc ggtgctcgaa gccaacaacg
      421 cgccgcgaat cgggcgcttc gggtggaaga gtcagcacgc cagcatggag tcatttgccg
      481 cggatgcgta tctgaacgag atgggcatca cgactccggt gctgcccgag gaaaacacct
      541 cgctgggtcg aaatgtgggg ttcccatcgc cttttgatcc cgtgccggat cccgaagatg
      601 acggcatcga cgtggtcgcg ttcgccaact tcatgcgtgc caccaaggcg ccgccgcgag
      661 gaccgatcaa ttcgacggtg cgcgccgggg agacgttgtt caattccgtg ggctgcggcg
      721 gctgccacat accgacgatt cgcaccgccg ctccgggcac gcgcatcaac ggtggggcga
      781 tccgtgtgcg cgacgcggtg ggcaacaaga tcattcatcc atacagcgac ttcctgttgc
      841 atgacatcgg cacgggcgat ggcattccag tgttgccggg tccggcattt gcttcgacgg
      901 cgacgcaaat ccgcacggcg ccgctctggg gactgcgtac gcgcaaccgg ctgatgcacg
      961 acgggctgtc gttcaccaag caggaggcga tctcgaggca tgcagggcag gctgccgggg
     1021 tgcgagcggc ctatgacgcg ttgacgacgg cacagaagga ccaggtgctg gccttcctcg
     1081 attctttgtg agctgaggga cgcagctcga ttcgaggcaa cggccgcggg tcaaaggact
     1141 gacccgcggc ccttgtgcta gccggcgtat gtgccgtgtt cgaactggat gaaacagaag
     1201 ccgtggccga acggatcacc gaagctgatg cagcgtgatc cgcgccagtc aacgtggccg
     1261 gtttcctgtt tcgcgccggc cgcgatcgcg cgagcggcgg ccgcatcgac gtccggcacc
     1321 accaggtcga agtgaaccgg agtccagtga cggccataac cacgctcgac tggcggcgcc
     1381 ttcacggcgg ccgttccgga tttcttctgc agcagataga gagtggccga ggcgccggtg
     1441 agttcggcga cgtcgtcgtc gagcgtgcgc gaatgcgtga gcccgagcgc ggcggtgtag
     1501 aaggcgatgg cggcgtgaag ctccggcacg tcgatgttga tgacgatctt cacgcgggcg
     1561 ctcctggcgc ggctggcggc ccaatggcga attgcatttc gaccaccgac tcatgcatgc
     1621 gttccggagt cggtagtttg agaaacacct cgcggctggg tcccgcgaga gtttcgccac
     1681 tggcggcaag gtattggcct atttttgcgg tcacgaggtg ggcatcttcc ggcaggccga
     1741 cgcgaacgca gactgccatg cgctcgacgc cttcgagtgt gcgccgggtc agcgaatgcc
     1801 ccgctcggcc ggccaccggc ggcattccgt cgaggacgta ggcgaactcg acatcgagtt
     1861 catcctgctc gaattgcggc gaatgcgcga tggccagcag cgtgggcgtg gttccgcggg
     1921 gcagcagcgg acgcgccagc tcgcgcagct cgaggatcag gcctcgcgcc gcggcgaatg
     1981 acggcaccgt ccggcgcagc gacaggattt cgcgggcggg ctcggcacgc acgatgacgt
     2041 cgtcggcgct cagtgttcct tcgctctcga gctgcgcgat ccgcgtttcg atctgctgca
     2101 gtcgccgcgc ctcgagcgcg agcgtctgct ccacatcgtt tcgacgcagc acgagcatgt
     2161 tgcgcagctc tgacggagcg atcttcgcgc gcaggatgtc gcgcacctgg tcgaggttga
     2221 atccgaggtc cttgagcacc aggatccggt tcaactcggc cagctgctcg atggcgtaat
     2281 agcgatagcc ggtctgttcg tccgcgtgcg ccggcacgaa cagaccgagc tcatcgtaga
     2341 accgcagcag gcgcgccgag acccgggcga tgcgcgagaa gtcaccgatc ctgaacattc
     2401 cagctccggt tcagcgccat ggagtcgacg acgggttgct cgcggcgcat ggcctcgacc
     2461 attggggggt gacgcatgac acggtcgaac aacgccgcaa ggcggcgaaa gcggccgcga
     2521 ggcagttcga agccgatgta ctgatagttg atcaggttgg agacgaccgc gaagtccgcc
     2581 acggtcggcc gatcgcccgc gagatagtcg ccacggatcg cggcatccag gaacccgaac
     2641 atttccggca cgacttcggc ctgaacctcg tcgacccgct tctggttcgt cgccacctgc
     2701 tgaatcctcg ggaagacaaa ggtttcctgg aacaacggtt gcaccacgtc gcgaaacagc
     2761 gcgcccgcat actgctcgaa tgacagcgcc gtggcgtact cgcggggcgc cgatggatag
     2821 accggttgtg ccggatgtat cttctcgagg taggcgcaga tcgctgccga gtccgccacg
     2881 gtgaaatcgc catccgtgag cgcgggtatt ttgcccgtcg ggctcaactc ccgccaattc
     2941 gccggcgggt tgccgggtat gacgggaacc acggggatga cttcgaattc gaggcctttg
     3001 gccagcgcca cgacgatgac tttgcgagtg tgaaccgaaa tgggaacgcc atagatcttg
     3061 agcatggtgc actccttgag ttgggagtgc ccaggctgcg gcttgacgtt acggcaaggt
     3121 caacaactat ttcgcgggtg gagaggcggc aaccgcgacc tcgacgtcgt gggcgacttc
     3181 ggcgcggatg cgttcccaat ggaaaaggtt gtcgggattc gcatcgaacg agttgccgtt
     3241 gtagtgagtg agcttcatgt cgcggtaaag gggccgggtg agggtgctga gctgcgtcca
     3301 gaagccgagc gcctgttgca gatggccgat tgcctgctgc ttgatttttt catcgcccgc
     3361 gatgcgatag ttggccaatg ccactgcgcc tcggagtttc tccgcgagat gaacgcccag
     3421 gtacgaccag gcctgcacat cggccacttc gtaacgcagc gaggcgttgt cagcagcttc
     3481 gatcaggttc accaggcggg ttgcctcgcg acagtcgcgt tcgagaacgt cggcaagctg
     3541 cagcggcgtg atgcgatccg cgccgaaggt gccgccggcg cgcatggtct tgacgtattc
     3601 cgcaacggat acataagtgg gatccagcgt cggctgatcg atgagcgcat cgacgccgat
     3661 gtaattcgta tgctcaccct gcaacgcaag cattccttcg ccgtagagag tgaagtccca
     3721 gcgtgagtca tacagcgatg cgagccgcat ttgcgtgttc gaggcgaggg agtaagcctt
     3781 caggaggttc tcgccgcgct tgccgtagcg gcgcgtgaat tcggcgtgga actgcgcgtc
     3841 gggcgttttc gggtcgtaca gcagccggcc ccagagcttg tagaacagcc actgacgctg
     3901 aaacgcccat ttccagtcga ccggttcctt gagcgccgta tgtgtgccgg cggtgaagta
     3961 atccagtgcg ggaatgtatg tttcggatcc aatgaaataa ccgccgacgt agtcctgcgt
     4021 tccattgagc gcgatgtgct gacgaatgaa gtccggcacg ccccagcgca gcgcgaagaa
     4081 atcttcgtta cgcacctgcc agacaatctt gtagttcgaa ggcgtgggtt tgaagtaggt
     4141 gtcgccgagc ttgccaccgt gcaccttcac cagcttgggg gtggagtggc catgcgacca
     4201 gttgaacttc atctctgccc agatgggccc tgcgaacttg tggccgagtt tctccatcgc
     4261 cgtgcgcgtg atttgctcga cttcggtgct gaccccgggc ccggacgaaa gccccgatga
     4321 aaacggcacg cgatggatga gtttcacggg ctgtttacgt ttcgtgttca gcgccccggc
     4381 gacatacacc tcgtcgacga actgctggcg ctggagcgga gtcatgccgc ccatgccctc
     4441 accatgcgac acgccgatgc cgtcgaggtc cgggtactcc tcgagcatct gcgtgacact
     4501 ctcgcgcaga taacgcttcg tgatgtcgga ggtgtcgccc ggcacgtagt agtgcgggta
     4561 gaagttttcc ttcgctacgt cgttcgcctt cgcgaactcc tcgctcacga agatgctcca
     4621 gaacaccacg taggtatcga gcccgcgttc tttcgccatg cgaaagatct cgcggtacag
     4681 gcgctgccat tcggcaaact ccgtgtccga ccacttgctc gcttcgggga agtttatcgg
     4741 ccgcaccatg taggtgaagg gatgcatggt ccacagcgtg aacgcgttga accggttctc
     4801 gaccatcatg tcgagccagg cttcccagaa cttcagatcg cgcgccgttg cgtagtgctg
     4861 atcgagcgcc gagctcgggc gataagtgtc ccacggcgta ttgaacttga tgccgcgaaa
     4921 gggcagtacg ggcttctcgc gcgaggcagt cacctggtcg atgggtgtgc cgttcaggag
     4981 ttgctcgcgc agcgcgagcg cgccatagat cagcccgcgc gcatcgccgc ccgtgatcgc
     5041 gatggtcttg ccgcgaggac tgatcgtgaa tgcttccggc gcgagaccct tcggcgatat
     5101 tttcagcgtc actttgtagc cgtcggacga agcgccgaac gcttccctca acttccgcgc
     5161 ggcgtaatca ctttgcggcg aggggttgtc gagaatcgaa atgctggcgg cgtggacatc
     5221 tgttgtgaca gcgagccaga caattgccca tcgaatggca acgtaaaaag gaattcgcac
     5281 ggttgttagg cgacggtagc ggtaacggtg cgcaaaggat acgcgaatcg cacggcaaca
     5341 cgtccgttcc ggccctctga cagatttttc cggtaaaacg ggattgcatt agtgatgccg
     5401 tcagtgcata aatccgcgca ggttcggtgg accaaaagcg ggactagagg tccggctcca
     5461 tccgggcgat cgatggattg atcacatcag ggggacccta tgtcacttcg cagaccttcc
     5521 gctgtgaaat ggcggagggc ggccgcgttc atcgcgctgc tgtccacgac cattttcgct
     5581 ttcctgtcgc cctcggcggc aatggcccag acgcttccga ccgcacccac cgttgccagc
     5641 cagatgaccg tcggctggaa cctcggcaac acgctcgaag ccatctgcag tgaaaccgcg
     5701 tggggcaacc cggccgcgag ccagacgctc atcaatgctg taaaggccgc cggtttcaat
     5761 tccattcgaa taccggtgtc ctgggactgc catacggcgc cctcaggcac gacgacaatt
     5821 cccgccgact ggatggcgcg cgtcaaacag gtggtcgact acgcgatcag ccaggacatg
     5881 ttcgtcatca tcaacatcca ctgggataac ggctggctgc aggatcatcc ggtcttcgcg
     5941 tcccagacgg cgataaacgc gaagcagcag gcgtactgga cacagatcgc caccaccttc
     6001 aggacgtacg accaccacct gctgttcgcc ggaacgaacg aagtgcacgc ggactacggc
     6061 acgccgaccg ccgaacacaa caccgtgcag cagtcgttca accagacctt cgtgaatgcg
     6121 gtgcgcgcca ccggcggcaa caacgcctcg cgctcgctcg tcgtgcagac gtacaacacg
     6181 aacatccagc acggcttcaa tttcttcaac atgccgacgg acaccatcgc cagccggctc
     6241 atggtcgagg ttcacaacta cgatccgtac gacttcacgc tgaacccgaa cggtgcctgc
     6301 aatgtgtggg gatcgcgctt cccggactcg ggcagcaact gcaactgggc ctatgagagc
     6361 tacttcgatt cgctcttcgc gcaggtgaag gcgaagtggg tcgaccaggg cattccggtg
     6421 atcctcggcg agtacggcgt cggcacgcgg ccgggcaaga acctcgaagc tcgcgcgtac
     6481 tggaacgagt acatcaacag ggccgccgcc gccaacggcg tgaagacctt ctattgggac
     6541 aacggcgcgc agcccggcag caacgatgcg ttcgcgatct tcaaccgcag cacgggcgcc
     6601 atcgtcgacc aggctgtgct cgac
//
LOCUS       DASQJS010000344         3050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_842129, whole genome shotgun sequence.
ACCESSION   DASQJS010000344 DASQJS010000000
VERSION     DASQJS010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842129"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..970)
                     /locus_tag="VEW08_13715"
     CDS             complement(197..970)
                     /locus_tag="VEW08_13715"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ41845.1"
                     /translation="MNGVTSSIASLMVGAAVTSSAAAAHRPPTIVLVHGAFVDGSGWA
                     GVHRILRTAGYPVVVVQHTTVSLAEDVAATRRAIASCDGEVILVGHSYGGVVITEAGS
                     NPKVSALVYIAAFAPDAGESVSALSANAPKNSGPPILPPNDGFLVLDKAKFAAAFAAD
                     VAPEIASFMADSQLPWGVQSLEGKITTPAWKTKPSWFLVPSEDHMIPPESQRGMAARA
                     GAQLREVSGSHAIYVSQPQAVVAWIEEAARKIPAAVTAR"
     gene            complement(967..1398)
                     /locus_tag="VEW08_13720"
     CDS             complement(967..1398)
                     /locus_tag="VEW08_13720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HYJ41846.1"
                     /translation="MSSIRSFFRGLVVVILASTAVPTFARDAEGVRYAAIPPGAYAVV
                     AHVRAKPGKEQELRAATLPLVAKVRAEPNNLLYFLHEDRETPGHFVFYEIFASQADFE
                     AHNATEHVQAWFAKLRELADGGVQVMRLEILGNLSSIGASK"
     gene            complement(1506..2321)
                     /locus_tag="VEW08_13725"
     CDS             complement(1506..2321)
                     /locus_tag="VEW08_13725"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ41847.1"
                     /translation="MNENPDTEDADIQRRSLFQAAGVALLGGLTVSTVAKPQTARGGT
                     SLGARLRGVQHFGVTVQDMERAFAFYTEVLGGTEVMRDGDFHGERIHNTLLTDQEIEA
                     RTRQVNPRALGIPDLRGGAQRLDVRFIQFDNVVIELLKYRDAEQASGGTAAFAEPLAH
                     MSPAFPRMMHICFHIGDEVDFNQFIADLEAESARRGMTQVRANRVVTVNSERDRLAAP
                     ISANSNGITEGKSNGWRLIYCKGPEGEQLEFVQALGPVKKVFDAARAARKALA"
     gene            complement(2314..>3050)
                     /locus_tag="VEW08_13730"
     CDS             complement(2314..>3050)
                     /locus_tag="VEW08_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41848.1"
                     /translation="RKVLLRLPRSWLPVGQPYGHVFAFDESGRIVEDLQDPTGAYTQT
                     TGVTETKDRRYIHSLNAPTIGWLPGVVSQAEAGGVEEIYVLRSIREASSGAAGNCAQE
                     LTGFAPMTADAEKMFSFWSVSTRAQDGRILDAHQAHVADLRGCFGATDERARQNFYAE
                     IKLGELAFHGNGECLALAVNFPESGLFPVRCQLTLKGLPAPYVGGMLTTNTMTSAAPM
                     GGESQPPGYTQASIATIRLWKERARE"
BASE COUNT          572 a          955 c          969 g          554 t
ORIGIN      
        1 actgagacgg catgagatgc cgtgagaaag gtaattggcg gagagagagg gattcgaact
       61 cggggaagcc gtgaatgaaa tcagtaagtt gctgaatcga atagcagcta gcgtcccctc
      121 agatccccct aactcccctt atttgccacc agatttgcca ccacgcaatg gcccggattc
      181 ccgaagtcga cgcctactat cgagccgtga ctgccgcagg gatcttccgc gccgcctcct
      241 ctatccacgc caccaccgcc tgcggctggg atacatagat cgcatggctt cctgagactt
      301 cccgcaattg cgcgccagca cgcgcggcca tgccacgctg cgattcaggc gggatcatgt
      361 gatcttcgct gggcacgagg aaccagctcg gcttcgtctt ccacgcgggc gtcgtgatct
      421 ttccttcgag cgactggacg ccccagggca gctgcgaatc tgccatgaag ctcgcaattt
      481 caggagccac gtccgccgcg aatgccgccg cgaacttcgc tttgtcgaga accaggaagc
      541 catcgttcgg cggcaggatc ggcgggccgg aattcttcgg cgcgttggcg ctcaacgcag
      601 acacggactc accggcatcg ggtgcgaatg ccgcgatgta caccagcgcg gaaaccttgg
      661 gattcgagcc cgcctcggta atgacgacgc cgccataaga gtggcccacg aggatgactt
      721 caccgtcaca cgaggcgatg gctcggcgag tggccgccac atcctcggcg agagataccg
      781 tggtgtgctg cacgaccacc acgggatagc cggccgtgcg caggatgcga tggacgcctg
      841 cccagcccga tccatcgacg aaagcaccgt gtaccagcac gatggtgggg ggacgatgag
      901 ccgcggcggc ggaagacgtg acggcggcgc ccaccatcag cgacgcgatg gaactcgtaa
      961 cgccgttcat ttcgaggctc cgattgaaga gaggtttccg agaatttcga ggcgcatgac
     1021 ctgcacgccc ccgtccgcaa gctcgcgcag cttcgcgaac caggcttgca cgtgctcggt
     1081 ggcgttgtgc gcctcgaagt ccgcctggct agcgaatatt tcgtagaaca cgaagtgtcc
     1141 cggtgtctcg cgatcctcat gaaggaaata cagcagattg ttgggctcgg cgcgcacctt
     1201 cgccaccagc ggcagggtcg cggcgcgcag ctcctgctcc ttgccgggct tggcgcggac
     1261 atgcgcgacc accgcgtagg cgccgggcgg aatcgcggca tagcgcacac cctccgcgtc
     1321 gcgtgcgaag gtgggtactg cggtcgatgc gaggatcacg acaaccaggc cccggaagaa
     1381 gctgcgtatc gatgacatgg agtgctcctc atgtggttgg caagcagttg ccgtgggagc
     1441 cgggtggcac gcgaactcct gacaaatcgg cgtgtgcacg ccaatgagat aaggattggg
     1501 aacattcatg cgagcgcctt gcgagcggca cgcgccgcgt cgaacacctt cttcacgggg
     1561 ccgagcgcct gcacgaactc gagctgctcg ccctccggac ctttgcagta gatgagccgc
     1621 catccattgg acttgccttc cgtgatgcca ttgctgttgg cagagatcgg cgccgcgaga
     1681 cggtcgcgtt ccgagttcac ggtgacgacg cggttcgccc tcacctgcgt catgcctctt
     1741 cgggcggatt ccgcctcgag atcggcgatg aactggttga agtcaacctc gtcgccgatg
     1801 tggaagcaga tgtgcatcat ccggggaaac gcgggactca tgtgggcgag aggttcggcg
     1861 aacgccgcgg taccgccaga ggcctgctcg gcgtcgcggt atttcagtag ttcgatcaca
     1921 acgttgtcga actggatgaa tcgcacgtcg aggcgctgcg cgccgccgcg caggtccggg
     1981 atgccgagag cgcgaggatt cacctgtcgc gtgcgcgctt cgatctcctg gtcggtgagc
     2041 agcgtgttgt ggatgcgctc gccgtggaaa tcgccatcac gcatcacttc ggtaccgccg
     2101 agcacttcgg tataaaacgc gaaagctcgt tccatatcct gaaccgtgac gccgaagtgc
     2161 tgtacgccgc gcaggcgcgc acccagggaa gtgccaccgc gcgcggtctg tggcttcgcg
     2221 acagtggaca cggtcaaccc tcccagcagc gcgactcccg cggcctgaaa gagactgcga
     2281 cgctggatgt cagcatcttc ggtgtctgga ttctcattca cgggctctct ccttccagag
     2341 acgaatggtg gcgatcgacg cctgcgtgta gccgggaggt tgggattcac cacccatggg
     2401 ggcggcgctg gtcatcgtgt tggtcgtcag cattccaccg acgtaaggcg ccggcagtcc
     2461 cttgagcgtc agctggcagc gaaccggaaa aaggccggat tcgggaaagt tcacggccaa
     2521 tgccaggcac tcgccgttgc catgaaacgc gagttcgccc agcttgatct cggcgtagaa
     2581 gttctggcgc gcccgctcgt cggtggcgcc aaagcagccg cgcaagtcag ccacgtgagc
     2641 ttggtgcgca tcgagtatgc ggccgtcctg cgcgcgcgtg ctcacgctcc agaacgagaa
     2701 catcttttct gcgtcggcgg tcatcggcgc gaagccggtc agttcctgcg cgcagttgcc
     2761 cgcagcgccc gatgacgcct cgcggatgct gcgcaagaca tagatctcct ctacgccgcc
     2821 agcctctgcc tggctaacta cacccggaag ccagccgatc gtgggagcgt tcaggctgtg
     2881 aatgtagcgc cgatccttcg tctcggtgac gccggtagtc tgcgtatagg caccggtcgg
     2941 atcctgcaga tcttcgacga tgcggccact ctcgtcgaag gcgaacacgt gtccataggg
     3001 ttgtccgact ggcagccatg accgcggcag ccgcagaagc accttgcgga
//
LOCUS       DASQJS010000345         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3040818, whole genome shotgun sequence.
ACCESSION   DASQJS010000345 DASQJS010000000
VERSION     DASQJS010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3040818"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="VEW08_13735"
     CDS             <1..1533
                     /locus_tag="VEW08_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41849.1"
                     /translation="LVTPAMLANKEVVKQLTIEQKIEFWRRVMQYARDRNIDFYVMTW
                     NIFTYGVEGKYGITDAIDNPKTIEYFRASVREMFRTYPLLRGIGLTAGENMGDASAYY
                     SGGMDSFDAKENWLFNTYGQGVSDAARAEPQRQIRLIHRQHETRAQDIARTFQPVITQ
                     PNVDFVFSFKYAQAHALSSTTQNFHRGYLESLGDLKTLWTVRNDDALMYRWAAPDFVR
                     EFMKNIPYEKSQGFYFGSDMWVWGREFLARDPKLQRQLEVDKHWLHFLLWGRLGYDPT
                     LDNERIAELVGRRFPGIDARKFLSAWQDASMVYPLVTGFHWANFDFQWYIEACRSRPD
                     PAKTASGFHSVNTFITQPVHPATRNITIPAYVAGVIAGKVPPGITPLQVADQLDERAD
                     RVLNSVAELNKSARGTMSAEYGATLADIMLMSSLGRYYAAKIRGATELALFRETLVKD
                     HRTKASQHLNDAARHWYQYSGRARVQYRNPVWTNRVGVVDWRELDAEVQRDIEIARGT
                     AP"
     gene            1679..2407
                     /locus_tag="VEW08_13740"
     CDS             1679..2407
                     /locus_tag="VEW08_13740"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HYJ41850.1"
                     /translation="MKRPVTLVLIATLLVGAPAFAAKDVQSKVADKVEKAVRHENGNR
                     GHDNRGHGNRGNDNRGNEHRGGSSQRNDNGHDRGNHAPPSYSGGGHDRGNDRNDHRND
                     NNRHDRNDHRYDNRNSNNRHDWNGNNGNRHDNRGWDRHRSDWSSHPNYWDNRRHDRRD
                     YARYRYNYGHYRAPHGYHYRTWHRGDYLPRAYYGSSYIVYDWHPYRLYAPPYGYHWVR
                     VGNDVLLTALATGVVLDVLYNIWY"
     gene            2564..2767
                     /locus_tag="VEW08_13745"
     CDS             2564..2767
                     /locus_tag="VEW08_13745"
                     /inference="COORDINATES: protein motif:HMM:NF016028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HYJ41851.1"
                     /translation="MTDRLEAIEIKLAHLERAVAEISDVVARQQKELDRALDRNQRLM
                     EKIAAIESESGASATAHEKPPHY"
     gene            complement(2786..3127)
                     /locus_tag="VEW08_13750"
     CDS             complement(2786..3127)
                     /locus_tag="VEW08_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41852.1"
                     /translation="MALPWLAVGSLVLGNLDKIMSVVRPGFTRKKVEVASAQFDLLNQ
                     QIAELQSAASTNAEQITQLAAQVKEVVAALAQAATEAAAHRASTRRWAYAAIAISVVA
                     ILLSAGVFAAR"
BASE COUNT          695 a         1015 c          960 g          527 t
ORIGIN      
        1 ctcgtgacgc cggcgatgct cgccaacaag gaagtggtca aacaactcac gatcgagcag
       61 aagatcgagt tctggcgccg cgtgatgcag tacgcgcgcg accggaacat cgatttctac
      121 gtgatgacgt ggaacatctt cacgtatggt gtcgagggca agtacggcat caccgacgcc
      181 atcgacaatc cgaagaccat cgaatacttc cgcgccagcg tgcgcgagat gtttcgcacc
      241 tatccgctgc tgcgcggtat cggactcacg gcgggcgaga acatggggga tgcgagcgcg
      301 tactactcgg gcggcatgga ctcgttcgac gccaaggaaa actggctatt caatacctac
      361 gggcagggag tgagcgacgc ggcgcgcgca gaaccgcagc ggcagatccg gctgatacat
      421 cgccagcacg aaacccgcgc gcaggacatc gccaggacgt tccagccggt gattacgcaa
      481 cccaacgtcg atttcgtgtt cagcttcaaa tatgcgcagg cgcacgcgct ctcatccacc
      541 acgcagaact tccaccgggg ttacctggag tcgttggggg atctcaagac actgtggacg
      601 gtgcgcaacg acgacgcact catgtatcgc tgggccgcgc cggatttcgt gcgcgaattc
      661 atgaagaaca tcccgtacga gaagtcccag ggcttctact tcgggtcgga catgtgggtg
      721 tggggccgcg aattcctggc gcgcgatccg aagctgcaac gccagctcga agtcgacaaa
      781 cactggctgc atttcctgct gtggggacgg ctcggttacg acccgacgct ggacaacgaa
      841 cgcatcgcgg aactggtggg ccggcgcttt cccggcatcg acgcgcgaaa gttcctgtcg
      901 gcgtggcagg acgcctcgat ggtctatcca ctggtcaccg gctttcactg ggcgaacttc
      961 gatttccagt ggtacatcga ggcctgccga agccggccgg atcccgcgaa gaccgccagc
     1021 ggatttcaca gcgtgaacac cttcatcacg cagccggtcc atcccgctac ccgcaacatc
     1081 accattcccg cgtacgtagc cggggtgatc gcgggaaaag tgccgccggg cattacgcca
     1141 ctgcaggtgg cagaccagct cgatgagcgg gccgatcgcg tgctgaattc ggtcgcggag
     1201 ctcaacaaga gtgcgcgcgg cacgatgagc gcggaatacg gcgcgacgct ggccgacatc
     1261 atgttgatgt cgagcctcgg caggtactac gcggcgaaga tccgcggcgc cacggagctc
     1321 gcgttgtttc gcgaaacgct cgtgaaggac caccggacaa aggcctcgca acacctgaac
     1381 gacgcggccc gccactggta tcagtattcg ggccgcgccc gagtgcaata ccgcaatccg
     1441 gtatggacga atcgagtggg cgtggtggac tggagggaac tcgacgccga ggttcaacgg
     1501 gacatcgaga ttgctcgcgg cacggcaccc tgaaaaccgc gaaaacaggc ctccggatag
     1561 cgttttctgg ctgaacaacc tgaacgaacc tgaacggaaa cgttcagcca gcgctcaggt
     1621 gggccgtccg atactggctc cgcaacggat cgacatggat ccgctaagga gaccagagat
     1681 gaaacgccca gtgacccttg tactcatcgc cacgctgctc gtgggtgcac cagcttttgc
     1741 cgccaaggat gttcaatcca aggtcgccga taaagtcgaa aaagccgtgc gtcacgagaa
     1801 cggaaatcgc ggacacgaca atcgtggtca tggcaaccgc ggcaacgaca accgcggcaa
     1861 cgagcatcgc ggtggatcca gccagcggaa cgacaatggg cacgatcgtg gcaatcatgc
     1921 tccgcccagc tacagcggtg gcggccatga tcgcggcaac gaccgcaacg atcatcgcaa
     1981 tgacaacaat cgccatgacc ggaacgatca tcgctacgac aatcgcaatt ccaacaatcg
     2041 tcacgactgg aacggcaaca atggcaatcg ccatgacaac cgtggctggg atcgtcatcg
     2101 cagcgactgg tcgagtcacc ccaactactg ggacaatcgc cgccatgatc gccgcgacta
     2161 cgcgcgctat cgctacaact acggccatta ccgcgcgccg cacggctatc actaccgcac
     2221 gtggcaccgt ggtgactacc tgccgcgcgc ctactacggc agctcgtaca tcgtgtacga
     2281 ctggcacccg taccggctct acgccccgcc gtacggttac cactgggtgc gcgtcggcaa
     2341 tgacgtcctg ctgaccgccc tcgccacagg cgtcgtgctc gatgtgctct acaacatctg
     2401 gtactgagcc gacctgaaac tcgagaaccc gagctagcgg gtagtcgccg gcgccctgga
     2461 atggattcca gggcgccgat gttttggggt caggcaccgt ttcctaggaa acggtgccag
     2521 gcaccgattg ctaggaaacg gtgcctgacc ccaaactgtc ggaatgaccg accgactcga
     2581 agcgatcgaa atcaagctcg cgcacctgga acgcgcggtg gccgagatca gcgacgtggt
     2641 ggcgcgtcag caaaaggagc tcgatcgcgc gctcgatcgc aaccagcggc tcatggagaa
     2701 aatcgccgcc atcgaatccg agtccggcgc gagcgccacg gcgcacgaga aaccaccgca
     2761 ctactgatcg tcgtcggcgt tccgctcaac gcgcggcgaa cacgccagcc gagagcagga
     2821 tggcgacgac cgagatggcg atcgccgcgt aagcccaccg cctcgtcgaa gcgcgatgcg
     2881 ctgcggcctc ggtcgcagcc tgtgccagcg ccgccaccac ttccttgacc tgcgcggcga
     2941 gctgcgtgat ctgttcggca ttggtcgaag ccgcgctttg cagttccgcg atctgctgat
     3001 tgagcaggtc gaactgggcg gacgcgactt ccaccttctt gcgcgtgaag cctggcctca
     3061 ccacgctcat gatcttgtcg agattgccca ggacgagact gccaaccgcg agccagggaa
     3121 gggccatgat tcacctccgt cgaaacatcc tggaactacg gcagaacgct gtacccgccc
     3181 gtctccgcaa actgatc
//
LOCUS       DASQJS010000346         6625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1155389, whole genome shotgun sequence.
ACCESSION   DASQJS010000346 DASQJS010000000
VERSION     DASQJS010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155389"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VEW08_13755"
     CDS             <1..641
                     /locus_tag="VEW08_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HYJ41853.1"
                     /translation="YYTNGQNAFIQFDPQANSNVLVLEARRDNPANYNCWYGRCEYTS
                     SRMNSAGKRTFQYGRIEARLKLPQTQGIWPAFWMLGTDIGSVGWPACGELDIMEHVGF
                     EPNITHGALHGPNYSGNTPFAGTNYLNEPVNANYHVYAIEWNSTSVRWFMDGVQFYSV
                     NKSQVQSFGNWVFDHPMFLILNVAVGGNWPGNPNGSSVFPQRMYVDYIRVYQ"
     gene            868..3546
                     /locus_tag="VEW08_13760"
     CDS             868..3546
                     /locus_tag="VEW08_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ41854.1"
                     /translation="MLKRGRNLFRRQYARLKRLDTLGSIGIVPRLAVAFMAVAALAAA
                     ANLIVENGISYIEQQRLLESERSANDSRAISSLRESMGRAQQIASSTQFLMALDRFDR
                     AVYEHAETDSKLSAARYSGARRDLERELSKYLADAHPASTLQLPRLIDAHMRSAEAFM
                     QLARVHRGLLTEYSTVFSRMEVRVEAAINGEWKVLGRVVAGESLLKLGAQLAVLRDAL
                     EARDTFGGGNTDNIALVKAEQGLADTLATNDTVLQRSQGSKWCQAMHADMAALLRVRT
                     SLLQAEPRRVAAAKGFVRETQKLTAMLPRPLEINAISEGESSPSSRTQAPAAAGTPAL
                     PEGPSRSLVAWLSAAVLVLLLYICFVTIFSIVRPVRRLVTATARLAKGENARVVASGG
                     IRELDTLAVAFNSMAEQLAAARASTRDAHQRLEDKVAERTRQLQLLAEQDPLTGLANR
                     RHLFAALDSTLARARVSGAHVGVFFLDIDNFKTLNDSMGHAYGDRVLIAIAQRLEVIA
                     RTYGFAARLGGDEFTIVHQGAASTEEVLACGAAIIRAFDVPVQVDFREVIVSVSVGAS
                     IFPDHEKDAESLLRAADAALFHAKALGRSQLATFTPELLTSASAKFAIEQRLRRAIQK
                     GEFELFYQPLVNAQTLEVQVVEALIRWRTPSGSFLAPGEFLAVAEESGLIMEISDWVL
                     RTAIETAAQWHQGAWRDVRVAINVSPRQFLNCGFVELLEKLLNEYQLPAQCVELELTE
                     SVLQTGAATINSLEQLRSIGVAIALDDFGTGYSSLASLQQLPLTRVKLDRSLIASMDG
                     SARSASIARATIGLCRDLGLEVTAEGVERLEQFAALRTYRNISLQGYLLARPVCREEL
                     LPLLERLPQHCQDLVRLSRELTPQAPAVRLATKRVS"
     gene            3620..4669
                     /locus_tag="VEW08_13765"
     CDS             3620..4669
                     /locus_tag="VEW08_13765"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HYJ41855.1"
                     /translation="MAINTPDEGSLDRDERKRLRSVLVRFQKPSTARALWQFFNTVVP
                     YVLLWVLMYHVKEISLWLAIPLAMLAGALLVRVFIFFHDCGHGSFFKSHWANAVFGFI
                     CGVLTFTPYYHWRWQHAIHHGTSGHLDKRGVGDIWTMTVQEYLESTRWNRFSYRFQRN
                     PFVLFVVAPLYIFLIRQRFASSDASPRERYSVWLMNFALLSVAIVMSWLFGFWIYLLL
                     QVIAWMVAGASGIWLFYIQHQFEDAYWERDEQWDYTAAALQGSSYYQLPRVLQWFSGN
                     IGFHHIHHLSSNIPNYNLERCHKADPVFQQVKPITLFSSFKSLRYRLWDEATRKLVSF
                     ARIRHLRREQRRRRP"
     gene            complement(4760..>6625)
                     /locus_tag="VEW08_13770"
     CDS             complement(4760..>6625)
                     /locus_tag="VEW08_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HYJ41856.1"
                     /translation="CHVSSGTTIDAAWMARVKTVVDYAYNQNMYVILNIHWDGGWLQD
                     NPTFAFQTANNAKQRAFWIQIANTFAAYNERLLFAGTNEVHADYGTPTSEHITVQQSY
                     NQTFVTAVRATGGNNASRTLVVQTYNTNAWHGLNYFSLPTDTIANRLIVEFHHYDPYD
                     YTINPSGPCLAWGAQFTQYSQCSWAQENYHTDLFAQIRNKWVAAGIPVIMGEYGVARR
                     SSLNDAARQYYLEFVNRTATTNGIKTFYWDNGAQSGASDVFALFDRNSGSIVDANGLN
                     AIMRGVGGTSTTFALAVTRSGTGTGTVTSSPSGINCGSTCSASYNSGTSVTLTAAAAS
                     GSTFGSWGGACSGTATSCTVSMTAARNVTATFNSSGANFTLSVTKAGSGTGTVTSNTG
                     NINCGSTCSASYASGTSVTLTAAAGSGASFAGWSGACTGTGACTVSMTQARAVTATFN
                     TASTFALGVTRAGTGTGTVTSSPSGINCGTSCTANYASGASVTLTAAAASGSTFAGWS
                     GSCSGTGSCVLSMTASRSVTATFNTSGGGTGGTCANPVTFTNQSGNFNTTGAVCYRTN
                     QNIAGWGCSNFDGRTLTVGGVARTCGQLPLTRSADGYYYFSVTAGQFAWASLYAW"
BASE COUNT         1210 a         2044 c         2088 g         1283 t
ORIGIN      
        1 agtactacac caacggccag aatgcgttca tccagttcga tccccaggcg aacagcaacg
       61 tgctggtact ggaggcccgg cgtgataatc cggcgaacta caactgctgg tacggtcgct
      121 gcgagtacac ctcgtcgcgc atgaattccg ccggcaagcg gactttccag tacggacgca
      181 tcgaagcgcg cctcaaactt ccgcaaaccc agggaatctg gccggcgttc tggatgctgg
      241 ggaccgacat cggatccgtg ggctggccgg cctgcggcga gctcgacatc atggagcacg
      301 tgggcttcga gccgaacatt acccacggtg ccttgcatgg cccgaactac tccggcaata
      361 cgccgttcgc gggtacgaat tacctcaacg aaccggtcaa tgccaactac cacgtctatg
      421 caatcgagtg gaactccacc agcgtgcggt ggttcatgga tggcgtccag ttctattccg
      481 tgaacaagtc acaggtgcag agttttggaa actgggtgtt cgaccacccc atgttcctga
      541 tcctgaacgt ggcggtcggc ggcaactggc cgggcaaccc gaacggcagc agcgtgtttc
      601 cgcagcgcat gtatgtcgac tacatccgcg tctatcaata gacgggaaat cccagccggc
      661 tagcaagccg gctggggctt tacgcgcgaa gtcggcgtac tcccacactc acgtatagga
      721 tttgacctac aggaaaactt gaactgcgca tgcagactcg tacgcgtttc gaagagcagt
      781 cgtcagaaat aatcgggccg cggcgggata ctcgccgggt cctagaatag aggctctaag
      841 ggagcggctt ggtccgacgc agactcgatg ctcaagcgcg gtcgaaatct gttcagacgc
      901 cagtacgccc ggctgaaaag gctggatacg ctgggaagta tcggcatcgt tccgcgactc
      961 gccgtggcgt tcatggcggt agcggcgctc gcggcagcag ccaatctcat cgtcgagaac
     1021 ggcatctcat acatcgagca gcagcgactc ctggagtcgg agcgcagcgc gaatgattcg
     1081 cgggcgatca gctcgctgcg cgaatcgatg ggtcgcgcgc agcagatcgc aagctcaact
     1141 caattcctga tggcattgga tcggttcgac cgggcggttt acgaacacgc ggagacggat
     1201 tccaagttgt cagccgcgcg ctactccggc gcccggcgcg atctcgagcg tgagctgagc
     1261 aaatacttgg cggacgcaca tccggcttcg acgctgcagc tgccccgcct gatcgacgcc
     1321 cacatgagaa gcgccgaagc gttcatgcag ctggcgcgcg ttcatcgcgg gttgctcacg
     1381 gaatattcga ccgtgttctc gcgcatggag gtgcgggtcg aagcagcgat aaatggtgaa
     1441 tggaaggttc tcggccgggt ggtcgcgggc gagtcactgc tgaaattggg cgcgcaactg
     1501 gccgtcttgc gggatgccct ggaggcgcgc gacaccttcg gcggcggcaa caccgacaac
     1561 atcgcactcg tgaaagcaga acaaggtctt gccgacacgc tggccaccaa cgatacagtt
     1621 ttgcaacggt cgcaggggtc gaagtggtgc caggcaatgc acgcagacat ggcggcactc
     1681 ttgcgcgtcc gtacgtcgct gctgcaagcc gagcctcgac gcgtggccgc ggccaaaggc
     1741 ttcgtgcgtg aaactcagaa gttgacggcg atgttgccaa ggccgctcga aatcaatgcg
     1801 atatccgagg gcgagtcgag cccttcaagc aggactcaag ccccggctgc agcgggaaca
     1861 ccggcattgc cggaaggacc gagccgatcg ctcgtggcgt ggttgagcgc cgcggtgtta
     1921 gtccttctgc tctacatctg cttcgtcacg atattcagca tcgtgcggcc cgtacgccgc
     1981 ctggtcacgg ccacggcgag attggcaaag ggcgagaatg cccgggtcgt tgcaagcggc
     2041 ggcatccgcg agctggatac cctggcggta gcgttcaatt cgatggccga gcagctggcg
     2101 gctgcgcgcg cctcgacgcg cgatgcgcac cagcggctgg aagacaaggt tgcggagcgc
     2161 actcgccaac tccagctcct cgcggagcag gatccactta cgggccttgc aaaccgcagg
     2221 catctgtttg ctgcgctcga ctcgacgctt gcacgcgcgc gcgtgtcagg cgctcacgtc
     2281 ggagtgttct tcctcgatat cgacaacttc aagactctca atgacagcat gggacacgcc
     2341 tatggcgatc gcgtcctgat tgcgatcgca cagcgactcg aggtgatcgc gcgcacttac
     2401 ggattcgcgg cgcggctcgg cggcgatgaa ttcacgatcg tgcaccaggg cgctgcctcg
     2461 accgaggaag tccttgcgtg cggcgcggcc atcattcgcg cattcgatgt cccggtgcag
     2521 gtcgactttc gcgaggtgat cgtgagcgtg agcgtcggcg ccagcatatt tcccgaccac
     2581 gagaaggatg cggagtcgct gctgcgcgcc gcggacgcgg cactgtttca cgccaaggca
     2641 ctcggacgca gccagcttgc aacgtttacg ccggaactgc tgacttccgc ctcggcgaag
     2701 ttcgccatcg agcagaggct gcgacgcgcc atccagaaag gtgagttcga gctgttctat
     2761 cagccgctgg tgaacgcgca gactctcgag gtgcaggtgg tcgaagcgct gattcgctgg
     2821 cgcacaccca gcggcagctt cctggctccc ggcgaattcc ttgccgtggc tgaggaatcc
     2881 gggctcatca tggagatcag cgactgggtg ctgcgcacgg ccatcgaaac cgcggcgcaa
     2941 tggcaccagg gggcatggcg cgacgtgcgc gtcgccatca acgtgtcgcc ccggcaattc
     3001 ctcaattgcg gattcgtcga attgctcgag aagctgctga atgaatatca gctgccggcg
     3061 cagtgcgtcg agctcgagct caccgagtcg gtactgcaga ccggagccgc gacgatcaat
     3121 tccctcgagc agttgcgcag catcggcgtc gcgattgcgc tcgacgactt tggcactgga
     3181 tattcctcgc ttgcgtcgct ccagcagttg ccgctcacgc gcgtcaagct cgatcgaagc
     3241 ctcattgcca gcatggatgg cagcgcgcgt tccgcgtcca tcgccagggc caccatcggg
     3301 ttatgccgcg atctcggcct cgaagtgacc gccgaagggg tggagcggct cgagcagttc
     3361 gccgcgctgc ggacgtatcg aaatatttcc ctgcagggct atctacttgc acggcccgtc
     3421 tgccgcgaag aactgctgcc gttgctcgag cggctccccc agcactgcca ggacctggtg
     3481 cggctctcgc gggagctgac tcctcaggct cccgccgtcc gactcgcgac caagcgcgtg
     3541 agttagggac aaggacgacg gtcaattcgg actttctcgg acgacggctc gcccggcccg
     3601 gacgtaagat ctcccgggca tggcaatcaa tactcccgat gaagggtcac tcgaccgcga
     3661 tgaacgcaag cggctgcggt ccgtgctcgt ccgttttcag aagccctcta cggctcgcgc
     3721 cctctggcag ttcttcaaca cggtcgtgcc gtatgtcttg ttatgggtat tgatgtacca
     3781 cgtgaaggag atttcgctgt ggctggcgat cccgctcgcg atgctggctg gcgcgctgct
     3841 ggttcgggtc ttcatcttct ttcacgactg cgggcacgga tcgttcttca agtcgcattg
     3901 ggccaatgcc gtgttcggct tcatttgcgg cgtcctcacg ttcacgccgt actaccactg
     3961 gcgctggcag cacgccatcc accacggcac gtcgggtcac ctggacaagc gtggcgtggg
     4021 cgatatctgg accatgacgg tccaggaata tctcgaatcc acacgctgga accgtttttc
     4081 ataccgcttt cagcgcaatc cgttcgtgct tttcgtggtc gcgccgctgt acattttcct
     4141 gatccggcag aggttcgcct cctcggacgc cagcccgcgc gagcgctact ccgtgtggct
     4201 gatgaacttc gcattgttgt ccgtcgccat cgtgatgagc tggctgttcg gattctggat
     4261 ctatctactg ctgcaggtga tcgcctggat ggtggctggc gcctcgggca tctggctgtt
     4321 ctacatccag caccagttcg aagatgcgta ctgggagcgc gatgaacagt gggattacac
     4381 ggccgcggcg ctgcaaggca gttcttatta ccagcttccg cgcgtcctgc aatggttctc
     4441 cgggaacatc ggtttccacc acattcacca cctgagctcc aacatcccga actacaacct
     4501 ggagcgttgc cacaaggcag accccgtctt tcagcaggtg aaaccgatca cgctgttctc
     4561 gagcttcaaa tcactgaggt accgactctg ggatgaggcg accaggaagc tcgtgagttt
     4621 tgcgcgcatc aggcatcttc gaagagagca gcggcgccgg cggccttagc gccgcatcaa
     4681 cacgggaaag ttcaggagct ctggcccgcc tgtgacagcg ggccagagtt ttcatcatca
     4741 atctacaact gcaaccgatc taccaggcgt acaggctagc ccaggcgaac tggcccgcgg
     4801 tcaccgagaa gtagtagtaa ccgtcggccg accgcgtaag tggcaactgc ccgcatgtgc
     4861 gagccacgcc gcccacggtg agcgtccgcc cgtcgaagtt cgagcatccc cacccggcga
     4921 tgttctggtt ggtccgatag cagaccgcgc cggtggtgtt gaaattgccc gattggttgg
     4981 tgaacgtgac cgggttggcg caggtgccgc cggttccgcc accgctggtg ttgaacgtcg
     5041 ctgtcaccga acgtgacgcg gtcatcgaca acacgcagct gcccgtgccc gagcacgaac
     5101 cgctccagcc agcgaacgtc gagccgctcg ccgccgcggc ggtcaatgtc acggaggcac
     5161 cactcgcgta gttagccgtg cacgaagtgc cacagttgat gccgctcggg ctcgaagtga
     5221 ccgtgccggt acccgtgccc gcgcgcgtga ccccgagcgc gaacgtggag gccgtgttga
     5281 acgtcgccgt caccgcacgc gcttgtgtca tcgacacggt gcacgcgccg gtgcccgtgc
     5341 aggcgccgct ccagcctgcg aagctggcgc cgctgccggc cgctgccgtc agcgtcaccg
     5401 aggtgccgct ggcgtagctc gcgctgcagg tgctgccgca gttgatgttt ccggtgttcg
     5461 acgtcacggt gccagtgccg ctgccggcct tggtgacgct gagcgtgaag tttgcacccg
     5521 agctgttgaa cgtcgcagtg acattgcgag ccgccgtcat cgacaccgtg catgaggtcg
     5581 ccgtgccact gcacgcgccg ccccaactgc caaacgtcga gccgctggcc gccgcggcgg
     5641 tcagcgtgac ggacgtaccg ctgttgtagc tggcgctgca ggtgctgccg cagttgatgc
     5701 ctgacggaga agacgtgacg gtcccggtgc cagtgccgct cctggtcacc gcgagcgcga
     5761 acgtggtgct ggtgccgccg acgccgcgca tgatggcatt gaggccattc gcgtcgacga
     5821 tcgagccgct gttgcggtcg aacagcgcga acacgtcgct ggcgccggat tgcgcaccgt
     5881 tgtcccagta gaacgtcttg atgccgttgg tggtcgcggt acggttcacg aactccagat
     5941 agtactggcg cgcagcgtca ttcaagctcg aacggcgcgc gacgccgtac tcacccatga
     6001 tcaccgggat gcccgccgcc acccacttgt tgcggatctg cgcgaacaag tcggtgtggt
     6061 agttctcctg ggcccacgaa cactggctgt actgtgtgaa ctgcgcgccc caggcgaggc
     6121 agggaccgct cgggttgatc gtgtagtcgt acggatcgta gtggtggaac tcgacgatca
     6181 gccggttggc gatcgtgtca gtcggcagcg agaagtagtt caggccatgc caggcgttcg
     6241 tgttgtacgt ctgcacgacg agcgtacggg aggcattgtt gccacccgtg gcgcgcaccg
     6301 cggtcacgaa tgtctggttg tacgactgct gaacggtgat gtgctcggac gtcggcgtgc
     6361 cgtagtcggc atgcacttcg ttcgtacccg cgaacagaag acgttcgttg taggccgcga
     6421 acgtgttcgc gatctgtatc cagaatgcgc gctgcttggc gttgttagct gtctggaatg
     6481 caaacgtggg attgtcctgc aaccagccgc catcccagtg gatgttcagg atgacgtaca
     6541 tgttctggtt gtaggcgtag tcgacgactg tcttgacgcg cgccatccat gccgcgtcga
     6601 tggtcgttcc cgaagagacg tggca
//
LOCUS       DASQJS010000347         6343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_843936, whole genome shotgun sequence.
ACCESSION   DASQJS010000347 DASQJS010000000
VERSION     DASQJS010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843936"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VEW08_13775"
     CDS             complement(<1..998)
                     /locus_tag="VEW08_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ41857.1"
                     /translation="MSHLELRGVSKSYGGTEVLSNINLSIEEGEFVALIGFSGSGKTT
                     LMSILAGLVRPDAGEVLLRGVAAGEAGPDRGLVFQSYSLMPWLTVFENVALAVDAVFA
                     KESREKRYARTEKYVRMVGLGHATDRRPAELSGGMRQRVAVARALAINPEFLLLDEPL
                     SALDALTRAKLQDEIEAIWREEKKTVVLVTNDVDEALLLADRIIPLNPGPRATLGPEF
                     RVNLPRPRDRKAVNHDPQYRQLRAAITQYLLDVEAEKPVPGEGAVKLPDVVPITSADF
                     LPKAVRAANKPKPLSNPDKYVEFYNVHKVYPTPRGPLTVVEGFQLDIKRGECVSLIG"
     gene            complement(995..2134)
                     /locus_tag="VEW08_13780"
     CDS             complement(995..2134)
                     /locus_tag="VEW08_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ41858.1"
                     /translation="MSSASNVKVLESPAAATAPTPPPAKLAAPPVASKPRFPRVNAAI
                     DAVIRALSLVGLGFLKPIVAITRGEDPRAHMRQLWLDLGAPVVAIAAFLLVWSQVSQG
                     IQTSLGTIPGPTAVWEQTKALWADHLVQREKAAAFYERQEKRNAEKLADDPSTDMTVR
                     RYTGKPTYIDQIFTSLKTVFAGFLLATLLAVPVGILCGASKTFNAMFNPLIQLFKPVS
                     PLAWLPIVTMIVSAVYVSADPLFDKSFINSAVTVTLCSLWPTVINTAVGVASIDRDLL
                     NVARVLRLKRSTQLTKLVLPSALPYIFTGMRLSLGVGWMVLIAAEMLAQNPGLGKFVW
                     DEFQNGSSESLSRIMVAVFTIGIIGFLLDRVMLALQSAFTYGPTR"
     gene            complement(2118..3506)
                     /locus_tag="VEW08_13785"
     CDS             complement(2118..3506)
                     /locus_tag="VEW08_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HYJ41859.1"
                     /translation="MSQNRPFVVRAVFATVIASLGFVMNASAAGKSPEKEQLKLGFIK
                     LTDMAPLAIAYEKGFFEDEGLYVTLEPQANWKVILDRVITGELDGAHMLAGQPLGASV
                     GIGTKADVVTGFSMDLNGNAITVSNEVWALMKAGLATDEKGLPKHPISATALKPALEK
                     FKASGKSFKMAMVFPVSTHNYELRYWLAAGGINPGFYAPSDVTGQTRADILISVTPPP
                     QMPATLEAGTINGYSVGEPWNQQAIFKGVGVPVITDYEIWKNNPEKVFGVTRAFADKN
                     PRTHIALVKALTRAAIWLDEKNGANRKEAAQILSRPEYVGADYRVIANSMTGTFEYGR
                     GDKRALPDFNVFFRYHANFPFYSDAIWYLTQMRRWGQIPDGKPDAWYIDTAKKVYRPE
                     VFVEAAKELVAEKKASAADFDFASDGFRAPTKDFIDGVEYDGRKPNAYIEKLVLGLKG
                     QQKVESGNVVGK"
     gene            3730..4572
                     /locus_tag="VEW08_13790"
     CDS             3730..4572
                     /locus_tag="VEW08_13790"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HYJ41860.1"
                     /translation="MIDWNLRDKTIAVPEMRELEVFSALLERRGAKVLRCPLVTIYDT
                     PHSAQVLAFAVKLADGGFDDFVLITGEGLTRILSCIDKYEPALRPRFIEGLGKLRTIT
                     RGPKPARALRVLGLKPGIEATEPTTNGVIRSLSALPLEGRRVAVQLYGNDPNLTLMGF
                     LRERLAITSTVAPYVYGNAADDATVHALLERMAAGEVDAIAFTSKLQIERLVTQHPAP
                     LVRRALTRTKIAAVGPIVAEAIRAAGFEVASSPEHSWFMKPLVVALSEQLGAKKDKDL
                     SGLA"
     gene            4662..6077
                     /locus_tag="VEW08_13795"
     CDS             4662..6077
                     /locus_tag="VEW08_13795"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HYJ41861.1"
                     /translation="MNRRELEMSAACAATLFALLALPQSADGAIKPDEAREISKEAYI
                     YGYPLVESYRILYSYFVNRSSPEYKAAWNEKVANNARVFTPADVAKGTPNADTPYSHL
                     GLDLRAEPMVLSVPAVQRDRYYSVEVNDLYTFIAGYLGTRAMGNEAGNFLIAGPNWKG
                     ERPPGIKSIINSETQLAFVFYRTQLFRPDDIENVMAIQAGYKVQPLSKFLGKTAPPPA
                     PRIDFFKPLSFAEQRTSLEFFNELNFVLQFCPTHPTEKALMSRFGKLNIGAGKDFDAN
                     ALSPEIRQAIEDGRADAWLAHDEILKRVTMGEIGSADVLGSRQALKNNYLYRMHGTVA
                     GIWGNSKEEAIYPGWYTDSSGKALDGSKGRYQVSFDAGQLPPVNAFWSVTMYGMPSHL
                     LVANPLNRYLVNSPMLPDLKGDADGGLTIHIQSTSPGKDKESNWLPAPNGPFVLVLRM
                     YWPKQEALNGAWKQPPLVRVN"
     gene            complement(6105..>6343)
                     /locus_tag="VEW08_13800"
     CDS             complement(6105..>6343)
                     /locus_tag="VEW08_13800"
                     /inference="COORDINATES: protein motif:HMM:NF025862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 97 C-terminal
                     domain-containing protein"
                     /protein_id="HYJ41862.1"
                     /translation="RKGKDWYVGAMGNEKPHAISVPLQFLGEGKFKAKLWEDGATPTT
                     LNESTRDVTSSDVLELKLAPSGGAAVRLGSGTNF"
BASE COUNT         1173 a         2020 c         1941 g         1209 t
ORIGIN      
        1 ccgatcagcg aaacgcactc gccgcgcttg atgtcgagtt ggaatccctc gaccacggtg
       61 agcggtccac gcggagtcgg gtagaccttg tgaacgttgt agaactcgac gtacttgtcg
      121 gggttggaca gcggtttcgg cttgttggcc gcgcgcactg ctttcggcag gaagtccgcc
      181 gatgtgatgg gcaccacgtc aggcagtttc acagcgcctt cccccggcac gggtttttcc
      241 gcttccacgt ccagcaggta ctgtgtgatc gccgcgcgaa gctggcgata ctgcgggtcg
      301 tggttcacgg ccttgcgatc tcgtggccgt ggcagattca cgcggaactc aggtcccagc
      361 gtggcgcgcg gtccggggtt gagcggaatg atgcgatcgg cgagcaacaa cgcctcgtcc
      421 acatcgttgg tcaccagcac gacggtcttc ttttcttcgc gccagatggc ttcgatttcg
      481 tcctgcagtt tggcgcgcgt cagcgcgtct agcgccgaca gcggttcatc gagcagcagg
      541 aactccgggt tgatggcgag cgcacgcgcc accgcgaccc gctggcgcat gccgcccgag
      601 agctccgcgg gacgccggtc ggtggcatgt cccagcccca ccatgcgcac gtacttctcc
      661 gtgcgcgcgt accgtttttc cctgctttct tttgcgaaca ccgcgtcgac cgccagcgcc
      721 acgttctcga aaaccgtgag ccatggcatc agcgaataac tctgaaatac caggccacga
      781 tccggccctg cttcaccggc cgcgacaccg cgcagaagca cctcgccggc atcgggcctg
      841 accagccccg cgagtatcga catgagggtg gtcttgcccg agcccgagaa gccgatcagc
      901 gcaacgaact cgccttcttc gatcgagagg ttgatgttcg acagcacctc cgtgccgccg
      961 taactcttcg acacgccgcg cagctcgaga tgactcatct cgtcggcccg taagtgaacg
     1021 cgctctgcag cgcgagcatc acgcgatcga gcaggaagcc gatgatgccg atggtgaaca
     1081 ccgccaccat gatccggctc agcgattcgg agctgccgtt ctggaattca tcccagacga
     1141 actttccgag accggggttc tgcgccagca tctcggcggc gatcagcacc atccagccca
     1201 ccccgagcga caggcgcatg ccggtaaata tgtagggcag cgccgaaggc agcaccagct
     1261 tggtcagctg cgtgcttcgc ttgaggcgca atacgcgcgc cacgttcagc aggtcccgat
     1321 cgatcgaggc aacgcccacg gccgtgttga tgaccgtagg ccagagcgaa cacagtgtga
     1381 cggtgaccgc ggagttgatg aatgacttgt cgaaaagcgg gtcggccgat acgtagaccg
     1441 cgctcacgat catcgtgacg atgggcagcc aggcgagcgg cgacaccggc ttgaagagct
     1501 ggatcaatgg attgaacatc gcgttgaacg tcttgctcgc gccgcacagg atgccgacag
     1561 gaaccgcgag caacgttgcc agcaggaatc cggcaaacac cgtcttgagg ctcgtgaaga
     1621 tctggtcgat gtaggtaggc ttgccggtgt acctgcgcac tgtcatgtcc gtggaggggt
     1681 cgtccgccag tttttcggcg ttgcgctttt cctgccgttc gtagaacgcc gcggcctttt
     1741 cacgctgcac caggtggtcg gcccacagag ccttggtctg ttcccacacc gcggtgggac
     1801 ccggaatggt tccgagactg gtttgaattc cctgcgacac ctggctccac accagcagga
     1861 acgcggcgat ggccacgaca ggtgcgccga gatccagcca gagctggcgc atgtgagcgc
     1921 gcggatcttc gccgcgcgtg atggcgacga tgggcttgag aaatcccagg cccaccagcg
     1981 agagcgcgcg aatcaccgca tcgattgccg cgtttacgcg cgggaagcgc ggcttggacg
     2041 cgacaggcgg tgcggcgagt tttgctggtg gtggtgtcgg cgcagtggcg gctgccggtg
     2101 actccagcac cttcacgtta cttgccgacg acattgccgc tctcgacctt ttgctgaccc
     2161 ttgagcccga gcaccagctt ctcgatgtag gcattcggct tgcggccgtc atattcgacg
     2221 ccgtcgatga agtccttggt gggcgcgcgg aacccatcgc ttgcgaagtc gaaatcggcc
     2281 gcactggctt tcttttcggc cacgagctcc ttggccgcct cgacgaacac ctcaggtcga
     2341 taaaccttct tcgcggtgtc gatgtaccac gcatccggtt tgccgtcggg aatctggccc
     2401 cagcggcgca tctgcgtcag gtaccagatg gcgtccgagt agaacggaaa gttggcgtgg
     2461 tatctgaaga acacgttgaa atccggcagc gcgcgcttgt cgccgcggcc gtactcgaac
     2521 gtgccggtca tgctgttggc gatgacgcgg tagtcggcgc cgacgtattc gggacgcgag
     2581 aggatctgcg ccgcttcctt gcgattcgcg ccattctttt cgtccagcca gatcgcggcg
     2641 cgtgtcagcg ccttgaccaa cgcgatgtgc gtgcgcgggt tcttgtcggc gaacgccctg
     2701 gtgacaccga acactttttc cggattgttt ttccagatct cgtagtcggt gataaccggc
     2761 acgccaacgc ccttgaagat ggcctgctgg ttccacggct ctccgaccga atatccattg
     2821 atggtgccgg cctcgagtgt cgccggcatc tgcggcggcg gcgtgactga aatcaggatg
     2881 tcggctcgcg tctgcccggt cacgtcagaa ggcgcataaa acccgggatt gatgccgccg
     2941 gcggcgagcc agtaacgcag ctcgtagttg tgcgtggaca cggggaaaac catggccatc
     3001 ttgaacgact tgcccgacgc cttgaatttt tcgagggccg gtttgagcgc cgtcgcggag
     3061 atcgggtgct tgggcagccc cttttcatcg gtagcgaggc ccgctttcat gagcgcccag
     3121 acttcgttgg aaaccgtgat ggcgttgccg ttgaggtcca tgctgaagcc ggtgaccaca
     3181 tcggccttcg tgccgatgcc aacagacgca ccgaggggtt gtcccgccag catgtgggcg
     3241 ccatcgagtt cgccggtaat gacgcgatcg agaatgactt tccagttggc ttgtggctcg
     3301 agcgtgacgt acaggccttc gtcttcgaag aacccttttt cgtacgcgat ggcgagcggt
     3361 gccatgtctg tgagcttgat gaaccccagc ttgagctgtt ccttttccgg acttttgccc
     3421 gcagccgagg cgttcatgac gaatcccagg ctggcgatca cggttgcgaa tacggctcga
     3481 acgacgaacg gacggttctg gctcactact aacttcctct cccatgcgcg gaagactcgc
     3541 gcggttgagg taaagcaatc ggcatgccag cgtttttttg gggtcatttt ttgcagccgt
     3601 ggccctgttt gcggcctcct gcaccgaaac gcggcggagg ggtgcactgc cttgcgcaaa
     3661 ccggtgcaga aagacccggg gtgtcgaggc gctcacagcc tccggacgag gtgatattgt
     3721 ccaaccccga tgatcgactg gaatctgcga gacaagacaa ttgcggtccc cgagatgcgc
     3781 gagctggaag tcttcagcgc attgctcgaa cggagaggcg cgaaagtcct gcgctgtccg
     3841 ctggtcacca tctacgacac gccgcacagt gcacaggtgc tcgcgtttgc cgtgaagctg
     3901 gccgacggcg ggttcgacga cttcgtgctg atcaccggcg aaggcctcac gcgcatcctg
     3961 agctgcatcg acaagtacga gccggcgttg cggccgcgtt tcatcgaggg cctgggcaaa
     4021 cttcggacga tcacccgcgg ccccaagccg gcacgtgcgc tgcgcgtgct gggactgaag
     4081 cccggcatcg aagccaccga gcccacgacg aatggcgtca ttcgttcgct ttcagcgctc
     4141 ccgctcgaag gccggcgcgt cgcagtgcag ctctacggca acgatcccaa cctcacgctc
     4201 atgggtttcc tgcgcgagcg gctggcgatc acctccaccg tcgcgccgta cgtctatgga
     4261 aatgccgccg atgacgccac ggtgcacgcg ctgctcgaac gcatggcggc cggcgaagtc
     4321 gatgccatcg cgttcaccag caagctgcag atcgagcggc tcgtgacgca gcaccccgcc
     4381 ccgctcgtgc ggcgcgcgct gactcgcacg aaaatcgcgg ccgtcggccc catcgtcgcc
     4441 gaggccattc gcgccgccgg cttcgaagtg gcctcgagtc ccgagcactc ctggttcatg
     4501 aagccgctgg tggtcgcgct gagcgagcaa ctgggcgcca agaaagacaa ggacctctcc
     4561 ggtctggcct gaatggggcg tgtgagcctc ccgagagcct aagggctcat tcccgcggct
     4621 ttccgttatc ctccacgcca agcaggttgg aggtgtgtcg catgaaccga cgcgaactcg
     4681 agatgagtgc cgcatgtgcg gcaacccttt tcgccctgct ggcgcttccg cagtccgcag
     4741 atggcgcgat caaacccgat gaagcccgcg agatctccaa ggaagcctac atctacggct
     4801 atccactcgt ggagagctac cgcatcctct actcgtactt cgtcaaccgc agcagtcccg
     4861 aatacaaggc ggcctggaac gagaaggtag ccaacaacgc gcgcgtattc accccggccg
     4921 acgtcgcgaa gggcactccc aacgccgaca cgccctactc gcatctcggg ctcgatctgc
     4981 gcgccgagcc catggtgctc agcgtgccgg cggtgcagag agaccgctac tactcggtgg
     5041 aggtaaacga cctgtacacg ttcatcgccg gttacctcgg cacccgcgcc atgggcaacg
     5101 aggcgggaaa ctttctgatc gcgggtccga actggaaggg agaacggcct ccggggatca
     5161 agtcgatcat caactccgaa acgcagctcg cgttcgtttt ctaccgcacc caactcttcc
     5221 ggccggatga catcgaaaac gtgatggcga tccaggcggg ttacaaagtg cagccgttgt
     5281 cgaaattcct cggcaagacc gcgccgcccc cggcgcccag gatcgatttc ttcaaaccgt
     5341 tgagcttcgc agagcaacgc acatcgctcg agttcttcaa cgagctgaac ttcgtgctgc
     5401 agttctgccc gacgcatccc acggaaaagg cgctgatgtc gcgcttcggc aagctcaaca
     5461 tcggcgccgg caaggacttc gatgccaacg cgctctcacc ggagattcgc caggccatcg
     5521 aggatggacg cgcggatgcc tggctcgcgc acgacgaaat tctcaagcgg gtcaccatgg
     5581 gcgaaatcgg cagcgcagat gtgctcggca gccggcaggc gctcaagaac aattacctct
     5641 atcgcatgca cggcacggtg gcgggcatct ggggaaattc caaagaggaa gcgatctatc
     5701 ccggctggta caccgactcc agcggcaaag cgctcgacgg atcgaagggc cgctaccagg
     5761 tcagcttcga cgccggccag ttgccaccgg tgaacgcgtt ctggtcggtc acgatgtacg
     5821 gcatgccgtc gcatctgctc gtggccaatc cactcaatcg ctacctcgtc aactcgccga
     5881 tgctccccga cttgaaaggc gatgccgatg gcggcctgac aatccacatc cagagcacct
     5941 cacccggcaa ggacaaggaa tcgaactggc tgcccgcgcc gaacggccct ttcgtgctcg
     6001 tgctgcgcat gtactggccg aaacaggagg cgctgaacgg cgcctggaaa cagccgccac
     6061 tcgtgcgcgt caattagatc ggaaatggtg ccaggcaccg ttttctagaa attggtgcct
     6121 gaccccagcc ttacggcagc gccgcccgac ggcgcgagct tgagctcgag cacgtccgat
     6181 gaagtgacgt cacgcgtact ttcgttcagc gtcgtcggcg tcgccccgtc ttcccaaagc
     6241 ttcgctttga acttcccctc acccaggaac tggagcggca cgctgatcgc atgtggcttt
     6301 tcgtttccca tggcgccgac gtaccagtcc ttgcccttgc ggc
//
LOCUS       DASQJS010000348         9942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_525938, whole genome shotgun sequence.
ACCESSION   DASQJS010000348 DASQJS010000000
VERSION     DASQJS010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525938"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VEW08_13805"
     CDS             complement(<1..396)
                     /locus_tag="VEW08_13805"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HYJ41863.1"
                     /translation="MQNALSYAELSHRIRAGDKPAEDQLSRRIAPGITQIIVGMTGNF
                     ALAQELCQETLIIILARLRSQPLDDPDKLPAFVAQIARNLVIAERRKDRRRKTDVDSE
                     AIEAVADHAQGQEQDAQRESAAVAIRKVLA"
     gene            508..3951
                     /locus_tag="VEW08_13810"
     CDS             508..3951
                     /locus_tag="VEW08_13810"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYJ41864.1"
                     /translation="MYSRFHFPLCVAALWGVAVAVAWAGETPVPFNAECEPTSGELTR
                     FDMNVNGQTRVTRTVTVGAGARVLVLARESRIDVRMEARSAGTRTISYADNPLRRWAI
                     QRITIDTAASRSFELAAVGKERAQGQVSVRVYSLDGFSPADDCLRALRASSDADASYA
                     RGQLINLATIAAAPGDAQRAYESAVDGYVRALALFKHRGATAAQARAETEHAATLYGL
                     RRYAEARVVAKRASAAFAVAGGDYGADRAKAIEAASEMEVARFEPGRGVAQSAAQARA
                     TEMLVHARALFSEVAKRHQARGELFEQALALNYIGLTFFYQDAYDEAIAGYQRALEVY
                     EKMGERMRQAQTLQNIGLVHNEMAQFPQAFETYARVLKLLDEAENPKLYADVLNNLAL
                     SEFETGRPDSALRHYSEALAILVRIQSKSEQARSLNGIGATYYRIGNRGEALKYFERA
                     LALRPASDDPVGRIASLRSAADILRDSGRWAEAFERRDEALRLARMPAQRARILVELA
                     RDAVDSGANERANAYFAELFRDDAAGDVGLYARALLERAKAGLARGTLDLAQRDVNEA
                     LSLFRAQQLLSSVFAGLVLQARIGCARGDAPAARTSIDGALDVAEQLRRSSNNPSLRA
                     SLWQPLRPAFDFKIEMISNPRMCGGGRQGDVALAALEVSERSRGRALEDFRLLGSLRG
                     ASSELSAPERKRAALYEQLAARRLQIESISESAADDDPRLKALRAEVSSLIRELDILG
                     GDIGSPLRGRGGMRAAVRNAIEMISPDATVVEYWLGEKNSYAWLATKGRTKMINLGST
                     ARIDASARALHHALRNFTSVRPEERLRLSRELHALIIDPLPAEALSAGTLYFVPDGTL
                     HTVPFAALVQGSGTPLRFLVDSRDIAVLPSIADAAVRRDKIPTGAGAGAVLVVADPVY
                     SRDDERFPRTAAAAPTRRAPALTLRGGAADDTTNGPQRWERLPAAGREAAAIVRLLEG
                     ADVEVLEGFAANRENLLKRNLQHYQVLHFATHSVADTEEPQLSALMLSTLDAAGTAIS
                     GEVFAGDLMLQKLRADLLVLSACDTALGQANTGEGLLGLRYAAHAAGARFVVASLWPV
                     ADTVGEQLMTGLYSAMTRRGKTPVQALSEAMRTTRARWSDPALWAVFEVSQAGSGETI
                     H"
     gene            3983..4480
                     /locus_tag="VEW08_13815"
     CDS             3983..4480
                     /locus_tag="VEW08_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41865.1"
                     /translation="MNLWEREWTINTGPDDVFLESGKTSFVLKKNGGNSFDVLFKRDE
                     MPAEWNDCSLVARGTEPFKLEGDPLPKFDESVKDKYSLAIKKALAKTTTSTRRLEGEL
                     DDGLERKTVYLFLARDAVGKVDESGDVTLHDLLVLKRATLFPWSSATAGAAPIDDGTA
                     HGDPK"
     gene            4477..5751
                     /locus_tag="VEW08_13820"
     CDS             4477..5751
                     /locus_tag="VEW08_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYJ41866.1"
                     /translation="MNTALKTSEVDANFDADTGEHVVVGKLLDTCPKATLSNCRRELA
                     LDYESFATTCADRDFRAITPGSAMHFEKLGLMLCATSKEQARKGNLFSRVEQIHAGAR
                     LRRLPQPPFKEADADNDNDDAVGTARSPADPQWGLREIGALDSEFTGKGVRVCVIDTG
                     FNIFHDDLKKVDAAHRRSFVGDSTVMSAHAGHGTRSAGIVGGPLRPAHGLRYGVAPDA
                     ELFVAKVLGKNGITIEALITALGWAVENQCAVVSMSIGDELPMTVFSSGAMELAASSA
                     LAAGTLVVAGAGNDSMRDKVIQPIDHPANCKSIMAVAAIEQTMKIAHYSNAGPPGGGE
                     PKVDITGPGSSRSASFPTRYKVESGTSIATPFVAGVAALFAEAFPDVRGQELWDLLCS
                     KARELPGHNARDFGAGLVRAPRKSDTSLLRAV"
     gene            complement(5777..6772)
                     /locus_tag="VEW08_13825"
     CDS             complement(5777..6772)
                     /locus_tag="VEW08_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HYJ41867.1"
                     /translation="MRAVFLDFGTVSHDDLDTAQLERVLPGITLHATSTDAEVDERIA
                     GVEFILTNKLNISRARMRAVPGLRFIGLTATGTNNVDLEAARELGIAVCNIREYCTVS
                     VVQHVLGSILLLTHRFREYGQAAIDGTWSRSEQFTVPGAPIRELSGKVLGIVGYGTLG
                     KAVANAARDALGMRVLVAERRGTRRTPATAAEAENAFNFPVRVTLDELLRVADVLSLH
                     CPLTPATTGMIGTRELALMKPDALLINTARGALVDLDALVAALKGGDLGGAAIDVLPN
                     EPPAEGSVLFEPGIPNLLVTPHIAWAAFEARQRAVDELALNVEDFLRGGRRGRVV"
     gene            complement(6781..>9942)
                     /locus_tag="VEW08_13830"
     CDS             complement(6781..>9942)
                     /locus_tag="VEW08_13830"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYJ41868.1"
                     /translation="RRRKAERLDRRQGASMSFLEFPIRRYQFTLVAFLCLVALGWYAF
                     MSVPREEDPYFKIPGFTVAAIYPGADPKDLERLIAKPLEDRFATLDDVLKMETSIRDG
                     VSFTAIEFLASTDADKKYDEVTRELNALRPEFPPEVQIIVRRFSPGLVNTVQIALLSD
                     EAPYRELEDHARELKDKLKTIDGVRTSESWAYPERELRVAVDLRRMSELNLSPAQLIG
                     AVQSENANIPAGFVDLGSRSFSLKTSGSYDSLDQVRDTVIASIDGRIVRVRDVADVTW
                     STAAYSYTGRYKGKRAVFVTANQKDGYNILDVRERVMAAANQFSAELPKRIKLEVGFD
                     QSENVRSRLNRLYFDFGIAISLVLLTLLPLGLRAAGIVMVSIPLSLSFGLACLYFLDY
                     SLNQLSIAGFVVALGLLVDDSIVVVENISRHMRMGKSRIQAALDGTRQIFVAILGCTA
                     TLIFAFLPLLALPGTAGKFIRVLPMAVVATIMGSLLLALFIIPFVASRVLSEEKEGQE
                     NRFLKRVMGVIHHYYRPALHWCLARPKLTVAAAIGGSLLLSAALVPVIGSSLFPKADT
                     PQFLIQVDSPNGTSLSETDRALRAVEDELAKMPEVKGWFANLGHGNPQIYYNHIQRND
                     ASNYAEVFVQLHEYDTDETPQRLQLLRERLERYPGAHIYVKEFQNGPPISAPISVRVI
                     GQDLDTIEKLSHRVEDLVKGTAGTRDVKNPLNVSRTNLKLRIDSRKAQQLGVQTAELD
                     RAVRLAVAGVTVGTFKDTTGEQYPIVVRTPITRQAEFAALEQVRVPSLGGTTLPLSQL
                     ASLEFEKAPTLIQRFNRERAMTIDADAKPGYNIGELTGQITKKLDQMDWPRGYTYDLG
                     GEDEASQEAFGGIGIAIIVAIFGIFAILVLEFGNFKSTLIVLTVVPLGVFGGLLMLLV
                     SGNDISFVASIGFVALVGIEIKNSILLVDFTNQLREQGMPLDEAIEQAGEIRFLPILL
                     TSATAIGGLLPLALQNIGLYSPMSWVIIGGLITSTLLARLVTPVMYKLIPPSIAVPSR
                     SGGTLAAPAGSAGDEASPAWRTS"
BASE COUNT         1814 a         3076 c         3288 g         1764 t
ORIGIN      
        1 agccaaaacc ttacggattg ccacggctgc ggactcgcgc tgggcgtcct gctcctgccc
       61 ctgggcatgg tcggcgaccg cttcgatggc ttcgctgtcg acgtcggtct tgcggcggcg
      121 gtccttgcgc cgctcggcaa tcaccaggtt ccgggcaatc tgcgcgacga atgccggtag
      181 tttgtcggga tcgtccagcg gctgcgagcg caagcgcgcc aggatgatga tgagcgtctc
      241 ctggcacagc tcctgtgcga gcgcaaagtt gccagtcatg ccgacgatga tctgggtgat
      301 gcccggcgcg atgcgccgcg acaattgatc ctcggctggt ttgtcgcccg cacgtatccg
      361 gtgcgatagt tcggcatacg agagagcgtt ttgcactgcg tgatcgtggc cgaacgctcc
      421 gatgtcgtaa aggtttacgt tgacgctggt ttgagggctc gggcagccta acgccgctca
      481 tcccgcattt gaacgacaag aacgggtatg tattccaggt ttcatttccc gctttgtgtc
      541 gcggcacttt ggggcgtcgc ggtcgcggtg gcatgggccg gagagacgcc agttcccttc
      601 aacgccgagt gcgagccgac gagcggcgag ctcacgcgct tcgacatgaa cgtcaatggc
      661 cagacgcgcg tgacgcgtac cgtcaccgtg ggagccggag cgcgcgtcct cgtgctggcg
      721 cgggaaagcc gcatcgatgt gaggatggaa gcgcgtagcg cgggcaccag gacaatttcg
      781 tacgccgaca atccgctgcg ccggtgggcg atccagcgaa tcacgattga tacggcggca
      841 tcacgcagct tcgagctggc cgccgttggc aaggaacgcg cacaagggca ggtgtcagtt
      901 cgtgtgtact cgctcgacgg gttcagcccg gccgacgatt gcctgcgtgc gctgcgcgct
      961 tcgtcggatg cggatgccag ttatgcgcgc ggacagctta tcaatctcgc caccattgcc
     1021 gcggctccgg gtgatgccca gcgtgcgtat gagagcgcgg ttgacgggta tgtcagggca
     1081 ctggcgttgt tcaaacaccg cggtgcaacg gcggcgcaag cgcgggccga gacggaacac
     1141 gccgctaccc tatatggcct gcgacgttat gccgaggcgc gcgtcgtcgc gaaacgcgcc
     1201 tcggcggcat tcgccgtcgc cggcggggac tatggcgctg atcgcgcgaa agcgatcgag
     1261 gcggcttccg agatggaagt cgcacgcttt gagcctgggc gtggcgttgc ccaatctgcg
     1321 gcacaagcgc gcgcgacgga gatgctcgtt cacgcccgtg cgctcttcag cgaagtcgcg
     1381 aagaggcacc aggcgcgagg tgagcttttt gaacaggcgc tcgcactcaa ctacatcggt
     1441 ctcacgttct tctaccagga cgcctatgac gaagccatcg ccggctacca gcgcgcgctc
     1501 gaggtttacg aaaagatggg cgagcgaatg cgccaggcgc agacgctgca gaacatcggg
     1561 ctcgttcaca acgagatggc gcagtttccg caggcctttg agacctacgc ccgcgtgctc
     1621 aaacttctcg acgaagccga gaatccaaag ctgtacgccg acgttctcaa caacctggcg
     1681 ctcagcgagt tcgagacggg tcggccggac tcggcgctgc gtcactactc cgaagcgctg
     1741 gcgatactcg tgcgaattca gtcgaagtcc gaacaggcgc gcagtctcaa cggaatcggc
     1801 gccacctatt accggatagg caatcgcggc gaagcgctga aatacttcga gcgcgcattg
     1861 gcgctgcgtc ctgccagtga cgaccccgta ggccggattg cttccttgcg ctcggcggcg
     1921 gatatcctgc gcgactcggg acgctgggcg gaagcattcg agcgccgcga cgaggcgctg
     1981 cgtctcgcgc gtatgcccgc acaacgcgcg cgaatcctcg tggaactggc gcgcgacgcc
     2041 gtggacagcg gcgccaacga acgcgcgaac gcctacttcg ctgaactgtt ccgtgacgat
     2101 gccgccgggg atgtcggact gtacgcgcgc gcattgctgg agagggcaaa ggccggtctc
     2161 gcccgcggga cgctcgatct cgcacagcgt gatgtcaacg aagcattgtc attgtttcgg
     2221 gcacagcagc ttctttcgtc cgtgttcgcc gggctggtgc tccaggcgcg catcgggtgc
     2281 gcgcgtggcg atgcgcctgc ggcccgaacg tccattgacg gcgccctgga tgtggccgag
     2341 caactgcgac ggtccagcaa caacccgtcg ctgcgcgcat cgctctggca gccgctgcgc
     2401 ccggctttcg atttcaagat agagatgatc agcaatcccc ggatgtgtgg cggcgggcgc
     2461 cagggcgacg ttgcgctggc cgccctcgag gtctccgagc gatcgcgcgg tcgtgcgctg
     2521 gaggattttc gcctcctcgg ctcattgcgg ggcgcctcat ccgaactgtc ggccccggaa
     2581 cgcaagcgcg ccgcgcttta cgagcaactg gccgcgcgcc ggttgcagat cgaatcgatc
     2641 tcggaatccg ccgcggacga cgacccgcgc ctcaaggccc tgcgcgccga ggtgtccagc
     2701 ctgattcgcg agctggacat cctcggaggc gacattggct cgcccctgcg cggccgcgga
     2761 gggatgcgag ccgcggttcg caacgcaatt gaaatgattt cgcccgatgc gacagttgtc
     2821 gagtactggc tcggggaaaa aaacagctac gcctggctcg caaccaaagg ccgcacgaag
     2881 atgatcaacc tgggaagcac cgcacgcatc gacgcgtccg cgagggcgtt gcaccatgcc
     2941 ctgcgcaatt tcacctcggt gaggcccgag gaacggttgc ggctgtcacg tgaattgcac
     3001 gcgctcatca tcgatcccct tcccgcagaa gcgctgagcg ccggcaccct ctacttcgtt
     3061 ccggacggca ccctgcacac ggtgccgttc gccgccctgg tgcagggcag cggaacgccg
     3121 ctgcgcttcc tcgtcgattc gagggacatt gcggtgcttc cttcgattgc cgatgcggcc
     3181 gtacgacgcg acaagattcc cactggcgct ggcgctggcg cggtgctcgt ggtggctgat
     3241 ccggtgtact cgcgcgatga cgagcgcttt ccgcgcaccg cggcggcggc gcccacacgt
     3301 cgcgcgccgg ccctgaccct gcgcgggggt gccgcagacg atacgacgaa cggcccgcag
     3361 cgttgggagc gcctgccggc ggctggccgc gaagctgcgg cgattgtgcg tctgctcgag
     3421 ggtgcggatg tcgaggtact cgaagggttc gcagccaatc gcgagaacct gctcaagcgc
     3481 aacctccagc actatcaagt cctgcacttc gccacgcatt cggtggccga caccgaagag
     3541 ccgcaactgt cggcgctgat gctgagcacg ctcgatgccg ctgggaccgc gatctccggc
     3601 gaagtctttg ccggcgacct gatgctgcag aaactccgcg cggacctgct ggttctcagc
     3661 gcctgtgata cggcattggg ccaggcgaat accggcgaag gcctgctcgg ccttcgatac
     3721 gccgcgcacg cggccggcgc gcgcttcgtc gtggcctctc tctggccggt ggccgatacg
     3781 gtcggcgaac agctcatgac cggtctgtat tcagccatga cgcgccgtgg aaagacgccc
     3841 gtgcaggcgt tgtccgaggc catgcgcacc acgcgggccc ggtggtccga tccggcgctg
     3901 tgggcggttt tcgaggtttc gcaggcggga agcggcgaaa cgatccactg actaactaca
     3961 aatctgatac ggggagtctt caatgaactt gtgggaacgt gaatggacca tcaacacagg
     4021 tcccgacgat gtattcctgg aatccggcaa gacgtcgttc gtgctcaaga aaaatggtgg
     4081 caactctttc gacgtcctgt tcaaacgcga tgagatgccc gcggaatgga atgactgcag
     4141 cctggtcgcg cgcggcacgg aacccttcaa gctcgaaggc gatccgctgc cgaagttcga
     4201 cgaatcggtc aaggacaagt acagcctggc gatcaagaaa gcgctggcca agacgaccac
     4261 gagcacccgg cgtctggaag gagagctcga cgacggcctc gagcggaaga cggtttacct
     4321 gttcctggcg cgcgacgcgg tgggcaaggt agacgagtct ggcgacgtca ccttgcacga
     4381 tcttctcgtc ctgaagcgtg cgacgctttt cccatggtcg agcgcgaccg cgggcgccgc
     4441 tccaatcgac gacggcacgg cgcatggaga tccaaaatga acactgcact caaaaccagt
     4501 gaagttgacg ccaacttcga cgccgataca ggcgaacacg tggtggtcgg caagttgctc
     4561 gacacctgcc cgaaggccac gctctcgaat tgccgccgcg aacttgccct cgactacgaa
     4621 agctttgcga cgacttgcgc cgatcgcgac tttcgagcaa ttacgcctgg cagcgcgatg
     4681 catttcgaga agctcggtct catgctgtgc gcaacctcga aggaacaggc gcgcaagggc
     4741 aatttgttct cccgcgtcga gcagattcat gcgggcgcca ggctgcgacg ccttccgcag
     4801 ccgccattca aagaggcaga cgctgacaat gacaatgacg atgccgtggg caccgcccgg
     4861 tcgccggcgg atccgcagtg gggactcagg gaaatcggtg cactcgacag cgagttcacc
     4921 ggaaagggcg ttcgcgtctg tgtgatcgat acggggttca acatctttca cgatgacctg
     4981 aagaaagtgg acgcggcgca ccggcgatcg ttcgtcggcg attcgaccgt catgagcgcc
     5041 catgcaggac acggaacccg gtccgcgggc atcgtgggcg gcccactgcg gccagcgcac
     5101 ggtctgcgtt atggcgtcgc gccagatgcc gagttgttcg tcgcaaaggt gctgggaaaa
     5161 aatggaatca cgatcgaagc gctcatcacc gcgctcggat gggcggtgga aaaccagtgc
     5221 gcggtcgtgt cgatgtccat tggcgacgag cttccgatga cggtgttttc gtccggcgcg
     5281 atggagcttg cggcctcgtc cgcattggcg gcgggcacgc tggtcgtggc gggcgctggc
     5341 aatgacagca tgcgcgacaa agtcatccag cccatcgacc accccgccaa ttgcaagtcg
     5401 atcatggcgg tcgcggcgat cgaacagacc atgaagattg cgcactactc caacgccggg
     5461 ccgccgggag gcggggaacc gaaggtcgat atcaccgggc ccggaagttc gcgttcggct
     5521 tcgtttccga cccggtacaa ggttgagtcg ggcacgagca tcgcaacacc attcgttgct
     5581 ggcgtggccg cgctctttgc ggaggccttt cccgatgttc gaggccagga gctatgggat
     5641 ctgctgtgct cgaaggccag ggaacttccg ggtcacaacg cgcgggattt cggcgcgggt
     5701 ctcgttcgcg cgccacgcaa gagcgacacc agcctgttga gggccgttta aggtggttag
     5761 gcggtaactt cgcgattcaa actaccctgc cgcgccttcc gccgcgcagg aagtcttcga
     5821 cgttcaacgc cagctcgtcc accgcgcgct ggcgcgcttc gaacgccgcc caggcaatgt
     5881 gcggggtgac gagcagattc ggaatgccgg gttcgaacag cacgctgcct tcggccggcg
     5941 gctcgttcgg caacacgtcg atggccgcgc cgccgaggtc tccgcccttg agcgcggcca
     6001 ccagcgcatc gagatcgacg agggcgccgc gcgcggtgtt gatgagcagg gcgtcgggct
     6061 tcatgagcgc gagttcccgt gtgccgatca ttcccgtggt cgcgggggtg agcgggcaat
     6121 gcaacgacaa gacgtcggcc acgcgcagca gctcatcgag cgtgacgcgc accgggaagt
     6181 taaacgcgtt ttcggcctcg gcggcagtgg ccggcgtacg gcgggtgcct cgtcgctccg
     6241 ccacgagcac gcgcataccg agagcgtcgc gtgcggcgtt ggcgacggcc tttcccagcg
     6301 tgccgtaacc gacgatgcca agaaccttgc ccgagagctc gcggatgggt gcgccgggta
     6361 ccgtgaactg ttcggagcgc gaccaggtgc cgtcgatggc ggcctggccg tattcgcgaa
     6421 agcgatgcgt aagaagaagt atcgagccga gcacgtgctg aaccacggat acggtgcagt
     6481 actcgcgaat gttgcagacg gcgatgccca gctcgcgtgc cgcttcgaga tcgacgttgt
     6541 tggtgccggt cgcggtcagg ccgatgaagc gcaggcccgg caccgcgcgc atgcgcgcgc
     6601 gcgagatgtt cagcttgttg gtgaggatga attccacgcc ggcgatgcgt tcatcgacct
     6661 ccgcgtcggt ggaggtggcg tgcagcgtga tgcccggcag caccctttcc agctgcgcgg
     6721 tgtcgaggtc gtcgtggctt acggttccga aatccagaaa taccgctctc atccagtccc
     6781 ctagctagtc ctccaggcgg gtgaggcttc gtcgccagca gagcccgcgg gggctgcgag
     6841 agtaccaccg ctgcgggagg gaacggcaat ggacggcgga atcagcttgt acatcaccgg
     6901 ggtgacgagg cgtgccagca gggtcgacgt gatgagaccg ccgatgatca cccaggacat
     6961 cggcgaatac agcccgatgt tctgcagggc cagcggcagg aggccgccga tggcggtcgc
     7021 cgaggtgagc aggataggca ggaaacggat ttcgcccgcc tgttctatgg cctcatcgag
     7081 cggcataccc tgttcgcgca actggttggt gaaatccacg agcaggatcg agttcttgat
     7141 ctcgatgccc accagcgcca cgaagccgat cgaggcgacg aacgagatgt cattgcccga
     7201 caccagcagc atcagcaggc caccgaacac gccgagtggc accacggtga gcacgatgag
     7261 cgtggatttg aaattgccga actcgagcac gaggatggcg aagatgccga agatcgcaac
     7321 aatgatggcg attccgatgc cgccgaaagc ttcctggctg gcttcgtctt cgccacccag
     7381 gtcgtaggtg tagccgcgcg gccagtccat ctggtcgagc ttcttcgtga tctggccggt
     7441 gagttctccg atgttatagc cgggcttggc gtccgcatcg atggtcatgg cgcgttcgcg
     7501 attgaaccgc tggatgagcg tgggcgcctt ctcgaactcg agcgaagcga gctgcgagag
     7561 cggcagcgtc gtaccgccga gggagggcac gcgcacctgt tcgagcgcgg cgaactccgc
     7621 ctgccgggtg atcggcgtgc gcaccacgat cgggtattgc tcgccggtcg tatccttgaa
     7681 cgtgccgacg gtcacgcccg caacggcgag gcgcaccgcg cgatcgagct cggcggtctg
     7741 cacgcctaac tgctgcgcct tgcgcgagtc gatgcgcagc ttgaggttgg tgcgcgagac
     7801 gttcaacgga ttcttgacgt cgcgcgttcc ggccgtgccc ttgaccaggt cttccacccg
     7861 gtgggagagt ttttcaatgg tgtcgagatc ctggccgatg acgcgcacgg aaatgggcgc
     7921 cgagatcggc ggcccgttct ggaattcctt cacatagata tgcgcgccgg gatatcgctc
     7981 gagccgttct cgcaatagct gcaggcgctg cggagtttca tcggtgtcgt attcgtgcag
     8041 ctgcacgaag acttccgcgt agttggacgc gtcgttgcgc tggatgtggt tgtagtagat
     8101 ctgcggattg ccgtgcccca ggttcgcgaa ccagcctttg acctccggca tcttcgccag
     8161 ctcgtcttcg accgcgcgca gtgcgcgatc ggtctcggac aacgacgtgc cgttgggcga
     8221 gtcgacctgg atcaggaact gcggcgtgtc cgccttcgga aacaggctgg atccgatcac
     8281 tggcacgaga gctgccgaca acaacaacga accaccgatc gcggccgcca ccgtcaactt
     8341 cggccgcgcg aggcaccagt gcaaggccgg gcggtagtag tggtggatga cacccatgac
     8401 gcgcttgagg aagcgattct cctggccttc cttttcctcg ctgagcaccc ggctcgcgac
     8461 gaatggaatg atgaacaacg ccagcagcag cgaacccatg atggtggcca ccacggccat
     8521 cggcaggacg cggatgaact tgccggctgt gcccggcagc gcgagcagtg gcaggaacgc
     8581 gaagatcaga gtcgcagtgc agccgagaat cgccacgaat atctggcgcg tgccatcgag
     8641 cgcggcctgt atgcgcgatt ttcccatgcg catgtgtcgg gagatgtttt cgaccacgac
     8701 gatggagtcg tcgaccagca gccccagcgc gaccacgaag ccggcaatgg acagctggtt
     8761 cagcgagtag tcgaggaagt agaggcaggc gaggccgaac gacaacgaca gcgggatgga
     8821 caccatgact atgccggccg cgcgcagtcc gagcggcagc aacgtcagca gcaccagtga
     8881 gatcgcaatg ccgaagtcga agtacaaccg gttgagccgc gagcgcacgt tctcggactg
     8941 gtcgaagccg acctcgagct tgatgcgctt cggcagctcg gccgagaact ggttagctgc
     9001 tgccatgacg cgttcgcgca cgtcgaggat gttgtagccg tctttctgat tggcagtgac
     9061 gaacaccgcg cgcttaccct tgtagcggcc ggtgtaactg tacgcggccg tgctccaggt
     9121 cacgtcagcc acgtcacgca cgcgcacgat gcgcccgtcg atggaggcga tgaccgtgtc
     9181 gcgcacctgg tcgagcgagt cgtaacttcc ggaggtcttc agcgagaagc tgcgcgatcc
     9241 caggtcgacg aagccggcgg gaatattcgc attctcgctc tgcaccgcgc cgatgagctg
     9301 cgcgggcgag agattgagct cggacatgcg gcgcaggtcc acggcgacgc gcagctcgcg
     9361 ctcgggatat gcccagcttt ccgacgtgcg cacgccgtcg atggtcttga gcttgtcctt
     9421 gagctcgcgc gcatggtcct cgagttcccg gtagggagcc tcgtcggaca gcagcgcaat
     9481 ctgcaccgtg ttcacgagtc ccggtgaaaa cctgcgcacg atgatctgaa cttccggcgg
     9541 gaactccgga cgcaacgcgt tcaattcacg tgtcacctcg tcgtatttct tgtccgcatc
     9601 cgtggacgca agaaattcga tcgccgtgaa cgacacgccg tcacggatgg aggtttccat
     9661 cttgagcacg tcgtcgagcg tggcgaagcg gtcttccagc ggcttggcga tgaggcgctc
     9721 gagatctttc ggatccgcgc ccgggtagat ggccgccacc gtgaagccgg gaatcttgaa
     9781 gtacggatct tcctcgcgcg gaacgctcat gaaggcatac cagccgagcg cgaccaggca
     9841 caggaacgcc accagcgtga actggtaacg gcgaatcgga aactcgagga acgacatgct
     9901 ggctccctgg cgccgatcga ggcgctcagc cttccgtcgc cg
//
LOCUS       DASQJS010000349         4406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2415694, whole genome shotgun sequence.
ACCESSION   DASQJS010000349 DASQJS010000000
VERSION     DASQJS010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2415694"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VEW08_13835"
     CDS             complement(<1..209)
                     /locus_tag="VEW08_13835"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HYJ41869.1"
                     /translation="MFRGAAKVTLDDKGRMVLPTRYREHALERSQGKLVVTVDRDQCL
                     LLYPLPDWEQIERKLMSLPSLHAQAR"
     gene            complement(595..963)
                     /gene="rnpB"
                     /locus_tag="VEW08_13840"
     ncRNA           complement(595..963)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VEW08_13840"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1029..1883)
                     /gene="rsmI"
                     /locus_tag="VEW08_13845"
     CDS             complement(1029..1883)
                     /gene="rsmI"
                     /locus_tag="VEW08_13845"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007989873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HYJ41870.1"
                     /translation="MAANLGRLAVISTPIGNLGDLSPRARDELAAAELVAAEDTRRTG
                     QLLTTLGLSRPLVSLHEHNEHERIDELLEKLRAGARIALVSDAGTPLLSDPGFELVRR
                     VAQEGMSVVAVPGPSAITAALSIAGLPTERFTFEGFLPARLGERRARVAELAAEPRTL
                     VFFEAPHRIAESLEDMAAGFGNARRAAVARELTKVFETVYRGTLSELATQARSDANFT
                     RGEITVVVEGAPRAAEDAAPRAQLDATLTVLLSELAPSKAAALAARLTGAKRNEAYAR
                     ALELAKQT"
     gene            1959..3812
                     /locus_tag="VEW08_13850"
     CDS             1959..3812
                     /locus_tag="VEW08_13850"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HYJ41871.1"
                     /translation="MRPFAFLVVVALAVVLTGCPTTPRRGGPPNVDRAAELARTGDHA
                     GAAALYERLADETTGSDSAEFRLRAARAWLAAGRAADADRVLALLPGGLTQQQALEQS
                     LLRIQSALVSGRGDEAWRQVSSMQTPTAQPAAARYYETRQQVAIATGHLLDGIRSELA
                     RERLIGPGDARLARSELLAQLRVAAERGVSLAPPPGSDATVRGWLEAASVALDNSRNP
                     TLGATRLAAFRARFPSHPALAALSGEPGVGIEEPVASLEAAPHLALMLPLTGRTSAPA
                     AQIRDGFMTAYYQVPAATRPRLRVYDTAITSIADTVAQAHTAGATFIVGPLTREEVVA
                     AGELLTTRPPILALNFLPADRPTPERYYQFALSPEDDARAVARYIGVTGKRRGVVLTP
                     EGDWGTRVAAAFDEELRTAGGFLLGQASFGGTTRNDFTASITQVLRTDDSNLRHQRIQ
                     SALGQKLDFEPRRRPDIQFIFAPSQPGAARQLRPQLRFHLAGDIPTFTLGDAYEPHPT
                     ANQEIDGVMFPDMPWMLGDEDLSGDVREAAREAFGDSERNRGRLFAFGYDAFRIATSL
                     QRGAPINPNGLTGTLSIDAQGRVRRELEWVRIKNGAPTPLERTDISATTPP"
     gene            3826..4173
                     /locus_tag="VEW08_13855"
     CDS             3826..4173
                     /locus_tag="VEW08_13855"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HYJ41872.1"
                     /translation="MDRRQTGTLAENSAAAFLESQGFTIVARNFQRRIGEIDVVARAG
                     ELLVVAEVRTRASDRFGGAAASISRGKQRRVAATTALFLQRRPELRRCRVRFDVIIVR
                     DGNVEWLRHAFEA"
BASE COUNT          744 a         1491 c         1355 g          816 t
ORIGIN      
        1 cgcgcctgcg catgcagcga aggcaggctc atcaacttcc gctcgatctg ttcccagtcg
       61 ggcaaggggt aaaggaggag gcattgatcc cggtccacgg tgacgaccag ttttccctgg
      121 cttctttcga gcgcgtgctc gcggtaccgg gtgggtaaaa ccatccggcc cttgtcgtcc
      181 agggtcactt ttgctgcacc gcgaaacatg gatggatagg ctcattgagc ccatttctcc
      241 ccactttttc ccacttgccg ccactatagg aatggccgtg ggcgcggtca agcaataagt
      301 cacgagattt tcccgtagcg acagcgactt acgcccgatt tgggtagtgg aagcgtgtga
      361 ttgtcgcgac gagcctctta tcgatcagtg gcttgtcatc gcttgttcat acagtgggta
      421 agaagcaggg acactgtatg tccctcttcg cgcgtcgagc tcgagcctgg cctccccatg
      481 ccatagagcg aagggatcaa agcgggagcg aaggaagaac aagatggctg ggtatccgtg
      541 gctattgaac cataaatggt tcaatagcca cggatacccg tttattagtt aaagaaaaag
      601 agtcggcctg taagccgggt tttgtcgagg acgatcattc ctctggaatc gccgtcaccg
      661 gcgacctcaa gcggcctacc cgaaagcacg gggggacacc cgctgccact tgcgtggcga
      721 ctttcctatt tggccttgct ccaggtgggg tttgccgtgc cgccgaatgt tgccaccggc
      781 gcggtgcgct cttaccgcac cgtttcaccc ttaccgtcgt cattgctgac gcttaggcgg
      841 tttattttct gttgcacttt ccgtcggctc acgccgccca ggcgttacct ggcaccttgt
      901 ccacaggagc ccggactttc ctccccattt cgcgagaaat gcagtgaccg tccggccgac
      961 tccgggcgca ccatagcggc atgtcgccac cttcacaagc cgcaggattt cgcctgcacg
     1021 ttgctgtctc aagtctgttt cgcaagctcg agcgcgcgtg cataggcctc gttgcgttta
     1081 gcgcccgtaa gccgtgcggc gagcgcggcc gccttgctcg gcgcgagctc gctcagcaag
     1141 accgtcagcg tcgcatcgag ctgcgcgcgc ggcgccgcgt cttcggctgc ccgcggcgcg
     1201 ccctccacca ccaccgtaat ctcgccgcgg gtgaagttcg cgtcgctgcg cgcctgcgtg
     1261 gccagttcgg acagcgtgcc tcgatacacg gtttcgaaaa ctttggtgag ctcgcgtgcc
     1321 accgctgccc ggcgggcatt gccgaacccc gcagccatgt cttcgagtga ttcggcgatg
     1381 cgatgtggcg cttcgaaaaa caccagtgtg cgcggttccg ccgcaagctc cgccacgcgt
     1441 gcgcggcgct cgccaagccg cgccggcaga aagccttcga aggtaaatcg ttcggtcggc
     1501 aggccggcga tgctgagcgc tgcggtaatg gccgaaggtc cgggcaccgc caccacgctc
     1561 atgccttcct gcgcgacacg acgtaccagc tcgaatcccg ggtccgacaa cagtggcgtc
     1621 ccggcgtcgc tcaccagcgc aatgcgcgcc cccgcgcgca gcttttccag cagctcgtcg
     1681 atgcgttcgt gttcgttgtg ttcgtgcagc gacaccagcg gccgcgacaa cccaagcgtt
     1741 gtcagcaatt gccccgtccg gcgcgtgtcc tcagccgcga caagctccgc ggccgcaagt
     1801 tcatcgcgcg cgcgtggcga aagatcgcct agattgccga tcggcgtcga gatcaccgca
     1861 agacgcccca agttcgctgc cactataatt ctccaccgcg ccaggatgcg caggcactcg
     1921 tgaaggctat tcgacgtatg acgcctaaga aagctctggt gagaccattt gcgtttttgg
     1981 tcgtcgtggc actggccgtg gtgctcacgg gttgcccgac cacgccccgc cgtggcgggc
     2041 caccgaacgt tgatcgcgcc gcagaactcg cccggaccgg agaccatgcc ggcgccgcgg
     2101 ccctttacga acgactggca gacgagacca ccggcagcga cagcgccgaa ttccgcctgc
     2161 gcgccgcgcg ggcgtggctc gccgccgggc gtgccgccga cgcagatcgc gtactcgcgc
     2221 ttctgccggg cgggctcacg cagcaacagg ctctcgaaca aagcctgttg cgtatccagt
     2281 ccgctctggt ttccggtcgc ggcgatgaag cctggcgcca ggtcagcagc atgcagactc
     2341 ccacggcgca gcccgccgcc gcgcgttact acgaaacgcg ccagcaagtc gccatcgcca
     2401 cgggccacct gctcgatggc atccgctccg agcttgcgcg cgaacgtctc atcggtcccg
     2461 gggatgcgcg ccttgcgcgc tcggagttgc tggcgcagct gcgtgttgcg gcggaacgcg
     2521 gtgtatccct ggctccgcca cctggcagcg acgctacggt acgcggctgg ctcgaagccg
     2581 cctcggtcgc gctggacaac agtcgcaacc caacgcttgg tgcgactcgc ctggctgcgt
     2641 tccgcgcgcg tttcccgtca catccggcgc tggccgcgct gtcgggcgaa cccggcgtcg
     2701 gcatcgaaga gccggtagcc agcctcgaag cagcgccgca ccttgcactc atgttgccgc
     2761 tcaccggacg cacttcggca cccgcggcgc agatccgcga cggcttcatg accgcctact
     2821 accaggtgcc ggccgccacg cgtccgcgcc tgcgcgttta cgacaccgcc attacttcga
     2881 tcgccgacac cgtggcccag gcacataccg ccggcgccac ctttatcgtc ggtccactga
     2941 cgcgcgaaga agtcgtggcc gccggtgaat tgctgacgac gcgtccgccg atactcgcgc
     3001 tcaattttct tccggccgac cgccctactc ccgagcggta ttaccaattc gcgctttcac
     3061 cggaagatga cgcacgcgcc gtggcgcgtt acatcggcgt caccggcaaa cgccgcggcg
     3121 tcgtgctcac acccgaagga gattggggca cgcgtgtagc cgcggcgttc gatgaagaat
     3181 tgcgcacggc gggtggcttt ctgctcggcc aggcgagctt cggcgggacg acgcgcaacg
     3241 atttcaccgc gagcatcacg caggtgctgc gcacggatga cagcaatctg cgccatcagc
     3301 gcatccagtc cgccctcggc cagaaactcg atttcgagcc gcgacgccgt cccgacatcc
     3361 agttcatctt cgcgcccagc cagcctggtg cggctcggca actccgcccg cagctgcgtt
     3421 ttcatctcgc cggcgacatt cccacgttta ccctgggtga tgcctacgag ccacatccca
     3481 ccgccaacca ggaaatcgac ggcgtgatgt ttccggacat gccgtggatg ctcggcgatg
     3541 aagatttgtc gggcgacgtt cgtgaagcgg cacgtgaagc cttcggcgac tccgagagaa
     3601 accggggccg cctgttcgcc tttggttacg atgcattccg cattgctaca tcgctgcagc
     3661 gcggcgcccc catcaatccg aatgggctca cggggacgct ctccatcgac gcgcagggtc
     3721 gtgtccggcg cgaactcgag tgggtccgca tcaagaacgg cgcgccgacg ccgctcgaac
     3781 gcacggacat ctccgcaaca acgcctccct gaaaatcccg ccggcatgga tcgccgtcag
     3841 accgggacac tggccgagaa cagcgcggct gctttcctcg aatcgcaggg tttcaccatc
     3901 gtcgcgcgca actttcagcg gcgaatcggt gaaatcgacg tggttgcgcg cgccggtgaa
     3961 ctcctggtgg tggccgaagt gcgcacgcgt gccagcgacc ggttcggtgg ggccgccgcc
     4021 agcatcagcc gcggcaagca acggcgagtc gccgccacca ccgcgctgtt cctgcagcga
     4081 cgccccgaat tgcgtcgctg ccgcgtgcgc tttgacgtga tcatcgtgcg cgacggaaac
     4141 gtggaatggc ttcggcacgc ctttgaagcg tgaagaggga gcgggttggg ggtgtgcgtg
     4201 ccccagcgag cagtccgcgc acaggatttc gttcgctttt cgccgggccc ttcgttcggg
     4261 gtggcatcgc cagattgctg gccgccgcca cacgcaatga cactttcgtg cggttggggt
     4321 ggaaagtcag cgagagtccg aatgaagcgg agaggcggct tcgtgcctaa cgaggccagg
     4381 tatcccggcc cgctctctca gctcct
//
LOCUS       DASQJS010000350         5576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2726450, whole genome shotgun sequence.
ACCESSION   DASQJS010000350 DASQJS010000000
VERSION     DASQJS010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2726450"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="VEW08_13860"
     CDS             complement(<1..1610)
                     /locus_tag="VEW08_13860"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYJ41873.1"
                     /translation="MAGINRISSASALAALSISVSVVAQQAPAPDAPKGEEVETVIVT
                     GIRESLRKGLENKKEATQVIESIVAEDIGKLPDNNVVEALQRVSGVQVTNRDGGEADG
                     ITIRGLPDITTTWNGRNVFTASGRALALQDIPANLVGQIDVYKTRAAEQLETGLAGQI
                     DVKTRRPFDLPGFEFSVNARAIQQEQRDTIDPNVSLLVSNQWEVGNDARFGALFNVSY
                     AETRYRTQNVISGALVPFATADAPPVGYGAAADNCPNTPPDNPNWTPLERIFNTNCRA
                     TLLGQPDPLLWQAGLDRGLSQEPGATLSIAGNQVPYLLARDALIASDFQGDRERPAAS
                     LALQFSPNDSSEYTFEAFYQGYREEMFNNLHFTFADWWGTLGPNPASTITLFPDTNII
                     KTRVVGAPFGFNSGDSTDQQTDTYVYALNGKWEITDTLRIVADLSFQDSEFNTNFIAM
                     RTTRVPAQITLDFNHNDGIPSWHFNNDEELLNPALWTAAELYQNRGRNKGDAKTFTLD
                     GDNSFADGSVFTHLKFGVRYDDRGASNE"
     gene            1996..2805
                     /locus_tag="VEW08_13865"
     CDS             1996..2805
                     /locus_tag="VEW08_13865"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HYJ41874.1"
                     /translation="MAVKKKSKKTKKAKKTVAAGPAHPHRKSLRLHGTIARDLGVLIV
                     SGRYRAGHILDGEVEASEQRKVSRTAYREALRILAAKGLVHSRPRVGTRVSPLENWHL
                     LDPDVLSWAFSGEPEPEVLHGLFELRTIVEPAAAALAAMRRNQKHLDSMRRALDAMAL
                     HTLNVEEGRIADKEFHAALLRATANPFIVSLTNGVTAAVNALTEFKQRIAPLKRDPAP
                     DHWRVYDAIAAKDAEGARVAMTELIRLAIMDTPIKQRPKPAAARVGANVHY"
     gene            2899..3930
                     /locus_tag="VEW08_13870"
     CDS             2899..3930
                     /locus_tag="VEW08_13870"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HYJ41875.1"
                     /translation="MSTGGLFTRRAFAGGLGLALAGIRSALAQTAGSINERMQGDFAP
                     VHDPCIIRQDDTYYLFSTSVKTDTAGFIACRRSRDLISWERAGFVFKEIPEWARDAVP
                     RTKGIWAPDISFFNGLYHLYYSVSSFGSNQSVIGLATNKTLDPAAPGFEWIDKGLVVR
                     SRLHDQYNAIDPNLAVDRDGKYWLSWGSFWDGLKLARIDPVTGKRMEGEDKMLSLASR
                     PARRGEPSPIEAPFIISHGDFYYLFASFDFCCRGAKSTYFVACGRARDITGPYLDVQG
                     RSLLEGGGSIVIQGNERFKGTGHNAVLRDGDQDYLVYHAYDAEHDGTPNLRISPIHWT
                     PDGWPRAQL"
     gene            3939..5516
                     /locus_tag="VEW08_13875"
     CDS             3939..5516
                     /locus_tag="VEW08_13875"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HYJ41876.1"
                     /translation="MTRIRKRWVGTAMLAVCASIAGSATARAADSAPVNVSVTIRADQ
                     PGPVINPNVYGQFAEHLGAGIYEGVWVGEKSAIPNTNGYRNDVIAALKNLKVPVVRWP
                     GGCFADEYHWRDGIGPREKRPVKVNTHWGGVPETNEFGTHEFMAFAEMIDTKVYISGN
                     VGSGSPQEMADWMEYMTSNTVSTLANERRKNGREQPWDVHFFGIGNETWGCGGNMTPE
                     YYANLFRQYATYVKAPRGKRPVIVASGGNDEHTQWVDVLTKNVHRDMDAISHHYYTTP
                     TGVWRKKGNSIGFPESEWISTLKHTMQIDQYIVDNVKILEERDPQNRTAFYVDEWGTW
                     YDPEPGREPGFLWQHNTLRDALVAGLNFNIFHKHARRVTMTNIAQMVNVLQAMILTDG
                     PRMALTPTYHVFQMYIPFQGATWLPAEITTPEYKLGNYAVPSVSVSAARDTEGRLQLA
                     LVNLDPNREAVVTTTLTGAKASGAQGRVLTSKTMDARNTVDAPEAITPVKISASRKGD
                     ALVLRLPPKSVSVVRLQ"
BASE COUNT         1089 a         1673 c         1771 g         1043 t
ORIGIN      
        1 ttctcattgg aagcgccgcg atcgtcataa cgcacgccga acttcaggtg cgtgaacacg
       61 gaaccgtcgg caaaggaatt gtctccgtcg agcgtgaacg tcttcgcgtc gcccttgttc
      121 ctgcccctgt tttgatagag ctctgcggcc gtccacagcg ccgggttgag caactcctcg
      181 tcgttgttga agtgccagga cggaatgccg tcgttgtggt tgaaatccag ggttatctgc
      241 gccggaacgc gcgtcgtgcg catcgcgatg aagttggtgt tgaactcact gtcctggaac
      301 gacagatccg cgacgattct cagggtatcg gtgatctccc atttgccatt caacgcatac
      361 acataggtgt cagtctgctg atcggtcgaa tcgccgctgt tgaatccgaa cggcgctccg
      421 accacgcgcg tcttgatgat gttcgtgtcc ggaaacaacg tgatggtgga tgccggattc
      481 gggccgaggg tgccccacca gtcggcgaac gtgaagtgca ggttgttgaa catctcctcg
      541 cgatagccct gatagaaggc ttcgaaggtg tattccgagc tgtcattggg cgaaaactgc
      601 aacgcgagac tcgcggcggg ccgctcgcgg tcgccctgga aatcgctggc gatcagtgcg
      661 tctcgcgcga gcagataggg gacctggttg ccggcgatgg acagtgtcgc gccgggttcc
      721 tgtgacaaac cacgatcgag acctgcctgc caaagcagcg gatcgggctg gccgagaagg
      781 gtggcacggc aattggtatt gaagatgcgt tcaagcgggg tccagttggg gttgtcgggc
      841 ggcgtgttcg ggcagttgtc cgctgcagct ccatagccca ccggtggagc atcggcggtc
      901 gcgaagggga cgagcgcgcc cgagatgacg ttctgcgtgc gatatcgcgt ttccgcgtag
      961 ctcacgttga agagggcgcc gaacctggcg tcgttgccga cttcccattg attgctgacc
     1021 agcaagctga cattcgggtc gatcgtgtca cgctgttcct gctggatggc gcgcgcattg
     1081 acggagaact cgaagccagg aaggtcgaaa ggacgccggg ttttcacgtc gatctggccg
     1141 gcgaggccgg tttcgagctg ttcggcggcg cgtgtcttgt agacgtcgat ctggccgacg
     1201 aggttggcgg ggatatcctg cagcgcgagc gcgcgccccg aggcggtgaa tacgttgcgc
     1261 ccattccacg tggtggtgat gtcgggcagg ccgcggatcg tgatgccgtc tgcctcaccg
     1321 ccgtcgcgat tggtaacctg aactccgctg acgcgctgca gcgcttcgac gacgttgttg
     1381 tccgggagtt taccgatgtc ttcggcaacg atggattcga tgacctgagt cgcttccttc
     1441 ttgttctcga ggcctttgcg aaggctctcg cgaataccgg tgacgatgac ggtttccact
     1501 tcctcgcctt tgggggcgtc gggagctggc gcctgctggg cgaccacgga gaccgagatg
     1561 gaaagcgccg caagtgcgga cgccgagctg atccgattaa ttcccgccac gttgtacccc
     1621 ctactgacgg acgaccttgg ctgagtccgg tctgtccgta tatttattac tcggacaaat
     1681 gctagcggca ggacaagcgg caatgcaatg tccgccgtgt catagctcca tgatcagact
     1741 gatatgtatg tcaggcactt acacacgccc ttaatactcg gacttttggt atttgttgat
     1801 tagtgaagaa gcttttatcg ttggcgggca aaggtacatg agccggttgt tatcaagggt
     1861 ttgcgagcgc atttgcgtgt ccgccggaca ccagcaatgg ccaatggaca gcagcaaaca
     1921 accttgtcgc cgcggggccc agcgcggaga atgccgccgc gttcgagata cacagtcttc
     1981 cggggaaatt cgggaatggc agtaaaaaag aagagcaaga agacgaagaa ggcaaagaag
     2041 acggtggcgg cggggcctgc gcatcctcac cgcaaatcgc tgcggctgca cggcaccatc
     2101 gcgcgcgatc tcggagttct catcgtttcc ggccgctatc gcgccggcca cattctcgac
     2161 ggcgaagtcg aagccagcga acaacgcaag gtgtcgcgca ccgcctatcg cgaggcgctg
     2221 cgcatcctgg ccgccaaggg gttggtgcac agtcgtccgc gcgtgggtac gcgcgtgagc
     2281 ccgctggaaa actggcatct gctcgatccg gacgtgcttt cgtgggcttt ttccggcgaa
     2341 cccgagccgg aagtgctgca tggcctgttc gagctgcgga ccatcgtcga gccggcagcg
     2401 gcggcgcttg ccgccatgcg acgcaaccag aaacacctcg acagcatgcg ccgcgcgctc
     2461 gatgccatgg cgctccatac gctgaatgtc gaagaagggc gcatcgccga caaggaattc
     2521 cacgccgcct tgctgcgcgc cacggccaat ccattcatcg tctcgctgac caacggtgtg
     2581 acggccgcgg taaacgcgct gacggagttc aagcagcgca tcgcgcctct caaacgcgac
     2641 cctgcgcctg atcactggcg cgtctacgat gcgatcgctg cgaaagatgc ggagggcgcg
     2701 cgcgtcgcaa tgaccgaact catcaggctc gcgatcatgg acacgcctat caagcagcgg
     2761 cccaagccgg cagcggcacg cgtcggtgcg aacgtgcact actagactaa agattgagta
     2821 cggcaggtcc gccttgcgcc tcgcggggtt tcgccgctag catcgatatt gtctgagtat
     2881 tgttcggtga aagctgtggt gagtactggc ggtctgttca cgcgtcgcgc attcgccggt
     2941 ggcctgggcc tggcgctggc cggcattcgc agcgcgctcg cgcaaacggc gggcagcatc
     3001 aacgaacgaa tgcagggcga tttcgcgccg gtgcacgatc cctgcatcat caggcaggac
     3061 gacacctatt acctgttttc caccagcgtg aagacggaca ccgccggctt catcgcgtgc
     3121 cgccgatccc gcgatctcat cagctgggag cgcgccggat tcgtgttcaa ggagatcccg
     3181 gagtgggcgc gcgacgcggt gccgcgcacc aagggtatct gggcgccgga catttccttc
     3241 ttcaacgggc tttatcacct ctattactcg gtctccagct tcggctcgaa tcagtcggtg
     3301 atcggcctcg ccaccaacaa gacgctcgat ccggccgcgc cggggttcga gtggatagac
     3361 aagggcctcg tggtgcggtc gaggctgcac gatcagtaca acgccatcga cccgaacctg
     3421 gcggtggatc gcgacggaaa atactggctc tcctggggca gcttctggga cggtctcaag
     3481 ctcgcgcgaa tcgatcccgt caccggcaaa cgcatggaag gtgaagacaa gatgctttcg
     3541 cttgcgtcac ggccggccag gcgtggcgag ccgagcccca tcgaagcgcc gttcatcatt
     3601 tcgcacggag acttctacta cctcttcgcg tcgttcgatt tttgctgtcg cggcgcgaag
     3661 agcacctact tcgtcgcttg cggccgcgcg cgcgacatca ccgggcccta cctggacgtg
     3721 caagggcgtt cattgctcga gggtggtggc agcatcgtga tccagggcaa cgagcgcttc
     3781 aaaggcaccg gacacaacgc cgtcctgcgc gacggcgatc aggattacct cgtgtatcac
     3841 gcctatgacg cggaacacga tggaaccccg aacctgagaa tttcacccat ccactggacg
     3901 ccggacggtt ggccgcgcgc acaactctag gagcaggaat gacgcgaatc agaaaaaggt
     3961 gggtcggcac cgcgatgctg gccgtatgcg cctccatcgc cggatcggcg acggcgcgcg
     4021 ccgcggactc ggcgcccgtc aacgtatcgg tgacgatacg cgccgatcaa cccggccccg
     4081 tcatcaatcc gaacgtctac ggccagtttg ccgagcatct cggcgccggc atctatgaag
     4141 gcgtatgggt cggcgaaaag tccgcgatcc ccaacaccaa cggctatcgc aatgacgtca
     4201 tcgctgcgct gaagaatctc aaggtgccag tggtgcgctg gcccggcggc tgcttcgccg
     4261 acgaatatca ctggcgcgat ggcatcggtc cacgggaaaa gcgccccgtg aaagtgaaca
     4321 cgcattgggg tggtgtgccg gagacgaacg aattcggcac gcacgagttc atggctttcg
     4381 ccgagatgat cgacacgaag gtgtacatca gcggcaacgt gggttcggga agcccgcagg
     4441 aaatggccga ctggatggaa tacatgacgt ccaacaccgt ctcgacgctg gccaacgagc
     4501 gccgcaagaa cgggcgcgag caaccctggg acgtgcactt cttcggcatc ggcaacgaga
     4561 cctggggttg cggcggcaac atgacgccgg agtactacgc caatctgttt cgccagtacg
     4621 cgacctacgt gaaggcgccg cgcggcaagc ggccggtcat cgtggccagc ggtggcaatg
     4681 atgagcacac tcagtgggtg gatgtgctca cgaagaacgt gcatcgcgat atggatgcga
     4741 tctcgcatca ctactacacc acgcccacgg gcgtctggcg gaagaagggc aactcgatcg
     4801 gtttcccgga atccgaatgg atctccacgc tcaagcacac catgcagatc gaccagtaca
     4861 tcgtcgacaa cgtgaaaatc ctcgaagagc gcgatccgca aaaccgcacc gccttctacg
     4921 tcgacgaatg gggtacctgg tacgacccgg agccgggccg cgaaccgggt ttcctctggc
     4981 agcacaacac gcttcgcgat gcgctggtcg ccgggctcaa cttcaacatc ttccacaagc
     5041 acgccaggcg cgtgacgatg accaacatcg cgcagatggt gaacgtgttg caggccatga
     5101 tcctcaccga cgggccgcgc atggcgctga cgcctaccta ccacgttttc cagatgtaca
     5161 ttccgttcca gggggctacc tggctgcccg cggagatcac cacgcccgag tacaagctcg
     5221 ggaactacgc cgtgccgtcg gtcagtgtct cggcggcgcg cgacaccgaa ggccggttgc
     5281 agctggcgct ggtgaacctg gatccgaatc gggaagcggt ggtcacgacc acgctcaccg
     5341 gagcgaaggc gagcggcgca cagggccgcg tgctcacgtc gaagaccatg gatgcacgca
     5401 acaccgtgga tgcgccggaa gcgattacgc cggtgaaaat cagcgcctcg cgcaaaggcg
     5461 atgcgctggt gctgcgattg ccgcctaagt cggtgagcgt ggtgcgattg cagtagttgc
     5521 gaggcccagg agtctggtcg cggtcctggt tgctgccatg gcggccgcga tcagcc
//
LOCUS       DASQJS010000351         9397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4299297, whole genome shotgun sequence.
ACCESSION   DASQJS010000351 DASQJS010000000
VERSION     DASQJS010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4299297"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VEW08_13880"
     CDS             complement(<1..902)
                     /locus_tag="VEW08_13880"
                     /inference="COORDINATES: protein motif:HMM:NF019631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase
                     domain-containing protein"
                     /protein_id="HYJ41877.1"
                     /translation="MRARLRPMVRVETVVLVVVAWLIATVNGAWWSAVATGRAWGDPA
                     NWLFIVAVFTALVALHFVLLGAVANRWTVRPLLTVIIIASAAAAYFMRTYAVIMDPTM
                     IRNILKTDAHEASELLSWSMVGSVLLWSALPVAFVWWARIEHRPWLRAVLLRTAHMAG
                     ALVVAVLAVLLVSRDLTSLMRNQRELRYLITPGNYIYGLAGTLARDARDARAPREPVG
                     ADARLIRVAMAHRPRVFVLVVGETVRAANFSLLGSYARPTTPELAKLDVVAFRNVTSC
                     GTSTEVSVPCMFSQWGREDYDERR"
     gene            complement(902..2605)
                     /locus_tag="VEW08_13885"
     CDS             complement(902..2605)
                     /locus_tag="VEW08_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41878.1"
                     /translation="MVGIRRPERSYLVVFAIGFALIGLCATQAQAKVLPSFKARAPKA
                     EPLRDADLPSDEELQRQGARIGEIRFDERDLFDTEAADEDTSMARLGNKLHITTRQST
                     IEDQLLFKSGDLYRPGLIEESARILRDTRYLRDALIRPVAFHDGVVDLEVMTQDVWTF
                     NPGISFGRKGGANSSGFELEELNFLGTGTQLGVGFKSDVDRDSKLIHFRDRQLGSSWW
                     DLDTGYSDNSDGRKAVFSLDHSFYSLDTRWAAGVSLLADQRTDSRYDLGEVVDQFQTH
                     EKFSTIYWGRSGGLRDGWARRLSVGLTYEDHAFGAVPGAATSPTLLPSGRTLVYPWIG
                     GEWVEDAFYTARNRNQIEKTEDYSLGWRARAQVGYATEALGSDRNAVMLAATATKGLE
                     LSERQTLSFGVDTRGRLESGGIAGGLFTADARYYFRQSPRRLLFLNLSATAGSNLDAD
                     QQILIGGDNGLRGYPLRYQSGEGRWLFTAEQRLFTNWYPFQLFNVGGAVFYDMGKSFG
                     RDPLGTPSQGLLRDVGFGLRLGTSRSALGNVLHMDVAYPLDGEDSVRKVQFLIETKKS
                     F"
     gene            complement(2645..4918)
                     /locus_tag="VEW08_13890"
     CDS             complement(2645..4918)
                     /locus_tag="VEW08_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ41879.1"
                     /translation="MNPRARPPRTRLQLICLLLTCCAVAAGQTTAPAQTVEAALQALR
                     QSGADVIYSSELVRPDMVAPPAHVGDTPLQRAVAALGANGLILRTIGPNTYVVMRAPA
                     APVAMSDEPMDEVSVYASRYSIAAGLAEPREVDVNDIERVPGSHDDAMRALHSLPGLA
                     SNASARPYIRGSLSEDVLVRYDGIPLLDPYHLKNFQSLISAIDPAGIERIEVFSGGFP
                     VQYGTRSGGVIDVIAPSYEQGHEFRASASLISAGVSSIGKAGNLPIEWLGAIRDSVLD
                     SIEQVEKGFGKPEFSDTLGRLRYLTDSGAWTVGWLLLDDRLELGQVDDEEQATARYRD
                     EYVWLARDHKFDDSLRTRASLVITSAERGRKGTLLNPGVATGSLDESRSFNGLDFSND
                     WTLRRSDTSTYTFGLQLAATRADYRYTRHSEFSPEVAAAFSRGLVEDLDYAVRPEIVT
                     YALYVANRRKWQHFEAELGLRLDAQHYEGGDDSNHTQVSPRLNLRYDFADEWRLYGSI
                     GRFTQAQHVEEWRVEEAQQSPDSAQVSIHSIVGLEYDTADGLRLGLEAYSKRWTTVVP
                     YFDNQLDPLALLPDLTPDRIRLDPHGSEARGLELSVRKPISDSFTGWGTLAWSRVADD
                     FPPRDVLRSWDQPLSVTAGLAWKGSNSSISALLGWHSGWPRTPVEFSPLQVEARNSRR
                     WGDFITLDLRGSWTWTYASGDLTAVLDLTNATNRRNECCLVLEMDEAAPVLAPEVDNW
                     LPAIINIGFTYRWRSSR"
     gene            complement(4893..5939)
                     /locus_tag="VEW08_13895"
     CDS             complement(4893..5939)
                     /locus_tag="VEW08_13895"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HYJ41880.1"
                     /translation="MSESNNTRNGDEGAAEHALREGLRAPTLSAEAMQRIRAAAQQEW
                     RAAHPPEPENAPVRAPARPRWLSLAVAASVAILAGVVGWNAMLGDAANSGAVVGQLAR
                     FEAPGIVESRFLRNDPVLDEGASLHAGQTLDVRGDSLITLAGGGNLRVARASTFEVVA
                     ANAVKLDHGELYVDIPPGSRGSDRFMVVTPAGEFRHVGTQFAVAIVNGMTRLRVREGS
                     VLWHATEGDSTVNAGTEVVIDRNRTVTRRPIATTGREWAWAESMAPDIEIENRPLQEF
                     LVWFSRETGRKLVLADDAARNHTATILMHGNVHGLTAMEALTAVMSATTLRFELPEGV
                     IRVSSARESTSPPT"
     gene            complement(5936..6544)
                     /locus_tag="VEW08_13900"
     CDS             complement(5936..6544)
                     /locus_tag="VEW08_13900"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ41881.1"
                     /translation="MSVLDEQDLIDRMRRGEQKAFDQFFDAFASRLAAFAARRCSLDA
                     AGLEDVVQVTMINAMRGLGSFRGGSALFTWLCQICRNHLADLRRKAERQPKVLSLEQI
                     EATKPPGTVVELTDFRDPLDEVADDSTRSAVRRAVNQLPATYARILELRFGDDLTVPE
                     IAKILQLSESAAESRLSRARQAFREGWSGGEESAAQGLREIP"
     gene            6622..7374
                     /locus_tag="VEW08_13905"
     CDS             6622..7374
                     /locus_tag="VEW08_13905"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYJ41882.1"
                     /translation="MDQSLNLPLPVLGPIERVPPRFDSPAFWRRHLWWPLLAFTIAFG
                     VLEIFSLDRILAREWYFNVHSSQWLGTGAGEWWARGVLHTGGRWVVRGVAAGALGLWA
                     LSFGIARLRPWRGSAGFVLLAMLLATLLVGGLKTVTNVDCPWDLIGYGGDNPYVALFA
                     DRPDALPHAQCFPGAHSSSGFALMCFYFVFLDRSRRTAAWALAAAMAVGVAFSIGQEA
                     RGAHFLSHDLASAAIVWFVQLGLYAWYRQRAA"
     gene            complement(7382..8737)
                     /locus_tag="VEW08_13910"
     CDS             complement(7382..8737)
                     /locus_tag="VEW08_13910"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ41883.1"
                     /translation="MTQHTTHETTAAMLAPLARGLLRRQPEILSLSIHDRHGHALWSS
                     GDFLLAEDHALIAEVIEDPHDVRGQSCGLWWESADTARARCALAIYEGGTFCGVALIA
                     FSGLTTCEEDRVTRVAELAPVLPVLAKAIYHSAGGAHEIVMASGLTEADTSGTTIIAA
                     LRFESHGADDSLVKDLETLAREERLLDLIDSALKEDEFALHLQPIVSLRDDASANGRP
                     LIVEVLIRLPTQEFGVLSSHEFLETAERNGRMPAIDRWVIRALLVWMQRNRDRWADAH
                     AVFSVNLSGKSVVRPDFRNYLEHCLDKSGLPLDSLRFEVAELDAGIAPQEFAALARSL
                     IDRGCEVSVDNAGTGTGRFDFLREVNADILKIDGSLVKGASDDIVSRALINGLVHMAD
                     TLGMRTVASQVDSTGTLRAVTQLGVDYAQGFRVHRPEDIETFDFVDCGIQRPLGVVAS
                     A"
     gene            complement(8862..>9397)
                     /locus_tag="VEW08_13915"
     CDS             complement(8862..>9397)
                     /locus_tag="VEW08_13915"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYJ41884.1"
                     /translation="LGAHQCNWEWMLLALSSQLGVPVDAAYKPLMDSWAECEMRKLRS
                     RLGARMIPAQDLLGDIIKQRGTPRGIAILADQEPKAGERKQWVRFLNRDTAFYVGPEE
                     IARSTRYPAFFLAIKRTSRGHYEVEFVKIAAAGEQLPPGEFTARYARMVEEQIRAAPA
                     DWPWSHKRWKLKKSLYG"
BASE COUNT         1688 a         3093 c         2966 g         1650 t
ORIGIN      
        1 atgcggcgct cgtcgtagtc ttcacggccc cattgcgaaa acatgcaggg caccgacacc
       61 tcggtggatg taccgcagga agtcacgttg cggaatgcga ccacgtcgag cttcgcgagc
      121 tcgggcgtgg tgggacgcgc gtagctgcca agcagcgaaa aattggcggc ccgcaccgtc
      181 tcacccacca ccagcacgaa cacgcgaggc ctgtgcgcca tcgcaacccg gatcaagcga
      241 gcgtcggcgc ccaccggctc gcgcggagca cgcgcgtcgc gagcgtcccg cgccagggtt
      301 ccggccaacc cgtaaatgta attgccgggc gtgatgaggt aacgcagctc gcgctgattg
      361 cgcatcagcg atgtcaggtc tcgggagacg agcagcaccg ccagcacggc caccacgaga
      421 gcacccgcca tgtgcgcggt tcgcagcagg accgctcgca gccacggccg gtgttcgata
      481 cgcgcccacc agacgaaagc caccggcaat gccgaccaca gcaggaccga gcccaccatc
      541 gaccaggaca aaagctcact ggcttcatgc gcatcggtct tcaggatgtt ccggatcatc
      601 gtcggatcca tgatcaccgc gtaggtgcgc atgaaatacg cggccgcggc gctggcgatg
      661 atgatcaccg tcaacagcgg gcgcaccgtc cagcgattcg ccaccgcacc gagcaacacg
      721 aagtgcagcg cgacgagcgc cgtgaaaacc gcgacgatga acaaccagtt cgcgggatcg
      781 ccccacgcgc gaccggtggc caccgcactc caccaggcgc cgttgacggt agcgatgagc
      841 caggcgacga ccaccagcac gaccgtctcg acacgaacca tgggccgcag gcgtgcgcgc
      901 atcagaaact ctttttcgtc tcgatgagga actggacttt ccgcaccgag tcctcaccgt
      961 cgagcgggta tgcaacgtcc atgtgcaaca cgttgccgag cgccgagcgg ctggtgccga
     1021 ggcgcaaccc gaatcccaca tcgcggagca gcccctgcga tggcgtgccc agcggatcgc
     1081 ggccgaagct cttgcccatg tcgtagaaca cggcgccacc gacgttgaac agctggaacg
     1141 gataccagtt ggtgaacagg cgctgctcgg cggtgaacag ccagcggccc tcgcctgatt
     1201 ggtagcgcag tggatagccg cgcagtccgt tgtcgccgcc tatcaggatc tgctggtcag
     1261 cgtcgagatt ggagccggcg gtggccgaca gattcaggaa cagcaatcgg cgcggcgact
     1321 ggcgaaagta atagcgcgca tcggcagtga acaggccacc cgcgatcccg ccactctcga
     1381 ggcggccccg cgtgtcgacg ccgaatgaca acgtttgtcg ctcggacagc tcgagcccct
     1441 tggtcgccgt ggccgccagc atcacggcgt tccgatcgga acccagcgcc tccgtggcgt
     1501 agcccacttg cgcgcgtgca cgccagccga gcgagtagtc ttccgtcttc tcgatctggt
     1561 tgcgattgcg cgcggtgtag aacgcatcct cgacccactc accgccgatc catgggtaca
     1621 cgagcgtgcg accggacggc agcagtgtcg gcgaagtggc cgcgcccggg accgcgccga
     1681 aggcgtggtc ttcgtaggtc agcccgaccg acagccggcg ggcccatcca tcgcgaagtc
     1741 cgccagaccg gccccagtag atagtcgaaa acttctcgtg cgtctggaac tggtcgacca
     1801 cctcgcccag gtcgtagcgg gaatccgtac gctggtcggc gagcaacgac acgccagccg
     1861 cccagcgggt atcgagcgaa taaaacgagt gatcgagcga gaacaccgct tttcgtccat
     1921 cgctgttgtc ggagtagccg gtgtcgaggt cccaccatga agagccaagc tggcggtcgc
     1981 ggaagtgaat cagcttcgag tcgcgatcca cgtccgactt gaagccgaca ccgagctgcg
     2041 taccggtacc gaggaagttg agttcctcga gttcgaaccc ggaggagttc gcgccgcctt
     2101 tgcggccgaa ggaaattccg gggttgaaag tccagacgtc ctgcgtcatc acttccaggt
     2161 cgaccacgcc gtcgtgaaac gcaacgggac ggatgagcgc atcgcgcagg taacgcgtgt
     2221 cgcgcaggat gcgagcggac tcttcgatga gccccggccg gtagaggtca ccgctcttga
     2281 aaagcagctg gtcttcgatc gtcgactgcc gcgtggtgat gtgcagcttg ttgccgagtc
     2341 gcgccatcga cgtgtcttcg tcggcggctt cggtatcgaa cagatcgcgc tcgtcgaagc
     2401 gaatctcgcc gatgcgcgca ccctgacgct gcagctcttc gtctgaaggg agatccgcgt
     2461 cgcgcagcgg ttccgccttc ggcgcacgtg ccttgaagct cggcagtact ttcgcctgcg
     2521 cctgggtggc gcacagcccg atcagggcga accctattgc gaatacaacg agatatgaac
     2581 gttctgggcg cctgattccg accatgcgcc gttagacgtc atgccggctg cgatcccgtc
     2641 gaagtcagcg tgagctgcgc cagcgatagg tgaacccgat attgatgatc gccggcagcc
     2701 agttgtcgac ttccggggcc agtactggcg cagcctcgtc catctcgaga acgaggcaac
     2761 actcgttgcg ccggttggtg gcattggtca gatccaagac tgccgtcagg tcaccgctcg
     2821 cataggtcca ggtccagctt ccgcgaaggt cgagcgtgat gaaatcgccc caccggcgtg
     2881 agtttcttgc ctcgacctga agcggcgaga attcgaccgg ggtgcgcggc caacctgagt
     2941 gccagcccaa cagtgccgat atgctggagt tcgacccctt ccacgccagg cccgccgtga
     3001 ccgacagcgg ctgatcccag cttcgcagga cgtcgcgcgg cgggaaatcg tccgcgacac
     3061 gcgaccacgc cagcgttccc cagccggtga acgaatccga gatcggcttg cgcacgctga
     3121 gttcgagtcc gcgcgcctcg gagccgtgtg gatcgaggcg gatccgatcc ggcgtcaggt
     3181 cgggcaacaa cgccagcgga tcgagctgat tgtcgaagta cggcaccact gtcgtccagc
     3241 gtttgctgta tgcctcgagc ccgagtcgca ggccgtccgc cgtgtcgtac tcgagcccca
     3301 cgatcgagtg gatagacacc tgggcggaat caggcgactg ctgcgcctct tccacgcgcc
     3361 actcctcgac atgttgtgcc tgcgtgaagc gaccgatgga gccgtacagg cgccactcat
     3421 cggcgaagtc gtagcgcaga ttgaggcgcg gactcacctg cgtgtggttg ctgtcgtcgc
     3481 cgccctcgta gtgctgtgca tccaggcgga ggccgagttc cgcctcgaaa tgttgccact
     3541 tgcgccggtt cgcgacatac agcgcatacg tcacgatctc cgggcgcacg gcgtagtcca
     3601 ggtcttcaac caggccgcgc gagaacgcgg cggcgacttc gggggagaat tccgagtggc
     3661 gtgtgtaccg atagtcggcg cgcgtcgcgg ccagctgcag gccaaacgtg taggtcgagg
     3721 tatcgctgcg tctcaacgtc caatcgttgg aaaaatcgag cccgttgaaa ctgcgggatt
     3781 catcgagcga gcccgtcgcc actccgggat tcaacaacgt acctttacga ccgcgctctg
     3841 cagaagtgat caccaggctc gcgcgggtgc gcaatgaatc gtcgaacttg tgatcgcgcg
     3901 ccagccacac gtattcatcg cggtagcgcg ccgtggcctg ctcttcgtcg tcgacctggc
     3961 ccagctcgag acggtcgtcg agcagcagcc agccgacggt ccacgcgccg ctgtcggtga
     4021 ggtagcgcag gcgtcccagc gtgtcgctga actccggttt gccgaagcct ttttccacct
     4081 gctcgatcga gtcgagcacg ctgtcccgaa tcgctcccag ccattcgatc ggcaggttcc
     4141 ccgccttgcc gatcgacgac acgccggcgg aaatcaggct ggcgctggcg cgaaattcgt
     4201 ggccttgctc gtacgacggt gcgatgacgt cgatcactcc ccccgagcgc gtaccgtact
     4261 gcacgggaaa gccgccgctg aacacttcga tgcgttcgat gcccgccgga tcgatggcgc
     4321 tgatgaggct ctggaaattc ttgaggtggt agggatcgag cagcggaatg ccgtcgtagc
     4381 gcaccagcac gtcttcgctc agcgaaccac gaatgtaggg ccgcgcggat gcattgctgg
     4441 cgaggcccgg cagcgagtgc aaggcgcgca tcgcatcgtc gtggctgccg ggcacgcgtt
     4501 cgatgtcatt gacatctact tctcgcggct ccgccaggcc agcagcgatc gaatagcggc
     4561 tcgcatacac cgacacttcg tccatcggtt catcggacat ggccacgggc gctgccggcg
     4621 cgcgcatgac gacgtaggtg ttgggtccga tcgtacgcag gatcaatcca tttgcgccaa
     4681 gagccgcgac ggcgcgctgc aacggcgtgt ctccgacatg cgcaggtggc gccaccatat
     4741 cgggacgcac cagctcggag ctatagatga cgtctgcgcc gctctgcctc aatgcctgca
     4801 gcgcggcctc gacggtttgc gccggcgcag tagtttgtcc ggccgcgact gcgcaacagg
     4861 tcagcaggag acagatgagt tgcaggcgtg ttctaggtgg gcgggctcgt ggattcacgc
     4921 gcggagctta cccggatcac gccttccggt aattcgaaac gcagcgtcgt ggcggacatg
     4981 acggcggtca gcgcctccat tgccgtcagc ccgtgcacat tgccatgcat gaggatggtc
     5041 gcggtatggt tgcgggcagc atcgtccgcc agcaccagct tgcggccggt ctcgcgcgag
     5101 aaccagacca ggaactcctg caatggacga ttttcgattt cgatgtccgg cgccatggac
     5161 tcggcccagg cccactcgcg cccggtagtg gcgatgggcc gacgcgtcac ggtgcgattg
     5221 cggtcgatca ccacttccgt gccggcgttc accgtggaat cgccttcggt cgcatgccac
     5281 aacacactac cttcccggac tcgcagccga gtcatgccgt tgacgatggc cacggcgaac
     5341 tgcgtgccca cgtggcggaa ttctccggcc ggcgtcacca ccatgaaccg gtcactgccg
     5401 cgcgaccctg gcggaatgtc cacatacagc tcaccgtgat cgagcttcac ggcattcgcg
     5461 gccaccactt caaacgtcga tgcgcgtgcg acacgcaggt tgccaccacc ggccagcgtt
     5521 atcagcgagt caccccgtac atcgagcgtt tgtccggcgt gcagtgacgc cccttcgtcg
     5581 agcaccgggt cattgcgcag aaagcgggac tcgacgatgc ccggcgcctc gaaacgcgca
     5641 agctgtccaa cgactgcgcc gctgttcgcg gcatcgccca gcattgcgtt ccagcccaca
     5701 acgcccgcga gaatcgccac cgacgcggcc accgcgagcg acagccagcg aggccgcgcg
     5761 ggtgcacgca cgggcgcatt ttcgggctcg ggcggatgcg ccgcgcgcca ttcctgctga
     5821 gcagccgcgc ggatgcgctg catcgcttcg gcggacagcg tgggtgcacg cagcccttca
     5881 cgcagtgcgt gttcggcagc gccttcatcc ccgttccggg tgttattcga ctcgctcatg
     5941 gaatttctct caaaccttgc gcggccgatt cttcgccgcc actccagcct tcacgaaagg
     6001 cctgccttgc ccgcgatagt cgcgactcgg cggcgctctc cgaaagctgc aggatcttcg
     6061 caatctccgg caccgtcagg tcatcgccga aacgcagctc gagaatgcgc gcatacgttg
     6121 ccggcaactg attcaccgcg cgccgtacag cgctgcgcgt ggaatcgtcg gcgacctcat
     6181 cgagcgggtc gcgaaaatcg gtcagctcca ccaccgtgcc cggcggcttc gtcgcctcga
     6241 tctgctccag gctcagcacc ttgggctggc gctcggcctt gcgacgcagg tccgccaggt
     6301 ggtttcggca tatctggcac agccacgtga acaacgctga gccgccgcga aaggatccga
     6361 ggccgcgcat ggcgttgatc atggtgacct gcaccacgtc ttcgagcccc gcggcatcca
     6421 gcgagcagcg tcgcgccgcg aacgcggcca gccgcgaagc gaaagcgtcg aaaaactgat
     6481 cgaaggcttt ctgttcacca cgacgcatcc tgtcaatgag gtcttgctca tccagcactg
     6541 acatacctcg ccaccttaga cgtcaccttt ctgagaaccc cgtcgcgacg gaaccgcagc
     6601 gaagattacg tctaagtcga ggtggaccaa agcctcaatc tccccctgcc cgttctggga
     6661 ccaatcgagc gagtaccgcc ccggttcgac agccctgcat tctggcgccg ccatttatgg
     6721 tggcccctgc tcgccttcac tatcgctttt ggtgttctgg agatcttttc gctcgaccgg
     6781 atactcgccc gcgagtggta tttcaatgtt cacagctcgc aatggctggg caccggcgcc
     6841 ggcgaatggt gggcacgcgg ggttttgcat accgggggtc gctgggttgt acgcggcgtg
     6901 gccgccggcg cactggggct gtgggcactg tctttcggga tcgctcgatt gcgtccctgg
     6961 cgtggatccg cagggttcgt gttgctggcg atgttgctcg ccaccttgct ggtgggcggc
     7021 ctgaaaaccg tcaccaacgt cgactgcccc tgggacctga tcggctacgg tggcgacaac
     7081 ccatacgtcg cactgttcgc cgatcgcccc gacgccctgc cccatgccca atgttttccg
     7141 ggcgcccatt cgtcctcggg attcgcgctg atgtgcttct acttcgtgtt tctcgatcgc
     7201 tcgcgccgca ccgccgcctg ggcactggct gccgcaatgg ccgttggcgt ggcattttcg
     7261 atcggccagg aagcacgtgg cgcgcacttt ctctcgcacg acctcgcgag cgccgccatc
     7321 gtgtggttcg tgcaactcgg gctatacgcg tggtatcgcc agcgagccgc ctgaagcgag
     7381 gtcaggcgct ggcaacgact ccgagcggac gctgaatgcc gcaatccacg aaatcgaagg
     7441 tctcgatgtc ctcgggccgg tgcacgcgaa acccctgcgc gtagtccaca ccgagttgcg
     7501 tgaccgcgcg cagggtaccc gtggaatcca cctggctcgc gacggtgcgc atgccgagcg
     7561 tgtcggccat gtgcacgagg ccgttgatca gcgcgcgcga aacgatgtcg tcggaagcac
     7621 ccttcaccag cgaaccgtcg atcttcagga tgtcggcgtt cacctcgcgc aggaaatcga
     7681 agcggcccgt gcccgtgccg gcattgtcca ccgatacctc gcaaccgcga tcgatgagcg
     7741 aacgcgcgag cgcggcgaac tcctgtggcg cgatgccggc atcgagctcg gcaacttcga
     7801 atcgcaggga gtcgagcggc aggccggact tgtcgaggca gtgctcgagg tagttgcgaa
     7861 aatccgggcg caccaccgac ttgcccgaga ggttcaccga aaacaccgcg tgcgcatcgg
     7921 cccagcgatc gcgattgcgc tgcatccaca ccagcaacgc gcggatgacc cagcgatcga
     7981 tggccggcat gcggccgttg cgctcggccg tctcgagaaa ttcgtgcgaa gaaagcacgc
     8041 cgaattcctg cgtgggaagg cgaatcagca cttcgacgat gagtggccgt ccattggctg
     8101 aagcgtcgtc acgcaacgaa acgatgggct gcaggtgcag cgcgaactcg tcttccttca
     8161 acgccgaatc gatgagatcg agcagccgct cttcgcgcgc gagcgtttcg aggtccttca
     8221 cgagcgaatc gtcggcgccg tgactctcga agcgaagcgc cgcgatgatg gtggtgccgc
     8281 tggtatctgc ttcggtcagc ccggatgcca tgactatttc gtgcgctcca ccggcggagt
     8341 ggtagatggc cttcgccagc accggaagca ccggcgccag ctccgccacg cgcgtcacgc
     8401 ggtcttcttc gcaggtcgtg agacccgaaa aggcgatcag cgcgacgcca cagaaggttc
     8461 caccctcgta gatggcgagc gcacaacgtg cgcgggcggt atctgcgctc tcccaccaca
     8521 ggccgcagct ttgcccgcgc acgtcatgcg ggtcttcgat cacttcggcg atcagcgcgt
     8581 ggtcctccgc gagcaggaag tccccactcg accagagcgc gtggccgtgc cgatcgtgga
     8641 tcgacaggga cagaatttcg ggttggcggc gtaaaaggcc acgcgccagc ggcgcgagca
     8701 tcgcagcggt agtttcatgg gtcgtgtgtt gcgtcatacg tggcccgaag attagggggc
     8761 cccggaggcg atcgcgtcgt cgcgaggatg cgacgtgggg ttcgggcgac taacagcgcc
     8821 gcggcgtgtt aggtcaaatc ggaaaatggg ggctgccccc actacccgta gagcgacttt
     8881 ttcagcttcc agcgcttgtg agaccacggc cagtcggccg gcgcggcgcg aatctgctcc
     8941 tcgaccattc gcgcatatcg ggccgtgaat tcgccgggcg gcagctgctc gcccgccgcc
     9001 gcgatcttca cgaactcgac ttcatagtgg ccacgcgagg tgcgtttgat ggcgaggaag
     9061 aacgcgggat atctcgtgct gcgcgcgatt tcctcggggc cgacgtaaaa cgcggtgtcg
     9121 cggttcagga accgcaccca ttgcttgcgc tcacccgcct tcggctcctg gtcggccagg
     9181 atggcaatgc ctcgcggcgt gccacgctgc ttgatgatgt cgcctaacaa gtcctgcgcg
     9241 gggatcatgc gcgcgccgag tcgcgaacga agcttgcgca tttcgcattc ggcccagctg
     9301 tccatcagcg gcttgtaggc ggcgtcgacc ggtacaccga gctgcgagga cagcgcgagc
     9361 agcatccact cccagttgca ctggtgcgcg ccgagca
//
LOCUS       DASQJS010000352         4736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2417680, whole genome shotgun sequence.
ACCESSION   DASQJS010000352 DASQJS010000000
VERSION     DASQJS010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417680"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VEW08_13920"
     CDS             complement(<1..387)
                     /locus_tag="VEW08_13920"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HYJ41885.1"
                     /translation="MRIEVVTLFPELIQQALKVGVLGRAVERGIVSVGCEDPRAHTRD
                     VHRTVDDRPYGGGPGMVLKPEPMAAAIDAAASRVPGGPRIYMSAQGVPFTQALAQEFS
                     KLPGLTLVAGRYEGLDERVIESRIDRE"
     gene            complement(396..899)
                     /gene="rimM"
                     /locus_tag="VEW08_13925"
     CDS             complement(396..899)
                     /gene="rimM"
                     /locus_tag="VEW08_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HYJ41886.1"
                     /translation="MKPSHIEVGRLGAAHGVRGWLRVQSFTDPPQRLFEWPTWQLQAA
                     NGATREAKLLEARAQGNGWIARLEGIDERDGASRLTGQMILVEREALPPAEGREHYRD
                     DLIGFEAKNLEGAVLGAVDHFVDTPGNAVMVIKGAREHWVPVTGQHLRSIDKDARTVL
                     VDWPEDF"
     gene            complement(933..1184)
                     /gene="rpsP"
                     /locus_tag="VEW08_13930"
     CDS             complement(933..1184)
                     /gene="rpsP"
                     /locus_tag="VEW08_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HYJ41887.1"
                     /translation="MVTIRLTRRGATNTPFYHVVVTDSRKRQGGKSLEQVGFFNPKAV
                     KNETKLKLDIARIDYWLSKGAQQSDRVKNLVTTFRKANA"
     gene            complement(1385..2749)
                     /gene="ffh"
                     /locus_tag="VEW08_13935"
     CDS             complement(1385..2749)
                     /gene="ffh"
                     /locus_tag="VEW08_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HYJ41888.1"
                     /translation="MALFENLTQRLSRTVDTLRGRGRITEENVGESLREVRMALLEAD
                     VALPVVKAFIERVKQKALGAEVTASLSPGQAFVGILHKELIALMSGDRGRFELRAQPP
                     VVVLLAGLQGAGKTTTAGKLARWLMESHKKRVLLVSTDVRRPAAILQLQRLAEQVGAG
                     FFPSDGKQKPADIARDALDAARKGVYDALIVDTAGRLHVDAELMEEVREIERAVETHE
                     RLFVIDAMAGQDAVNSARAFGAALDLTGVILTKADGDARGGAALSVREVTGKPIVLVG
                     VGEKLDALEPFDAERMAGRILGMGDVVALVEQVQKQVNVEEAQKLASKVAKGKGFDLV
                     DLKSQLEQLQKMGGVGALMDKLPQAAMKGAALPADGGDKDVRRQIAIINSMTRKERRN
                     PGLLDGSRRRRIAAGAGVQVQDVNKLMKQFMEMQKMMKSMAGGRMKRMLQAFKGGMPP
                     GFPQ"
     gene            2787..4067
                     /locus_tag="VEW08_13940"
     CDS             2787..4067
                     /locus_tag="VEW08_13940"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HYJ41889.1"
                     /translation="MQNAPSSILGIALVVLLLLIAFSSGTEVAMLSANRYKIRAAAKA
                     GKRTAKALDALLSRPESWLGANLVLLALASVAASSIATLLAQRTRYEHAIPVTMAVLS
                     IVMIVFCELAPKIYAALKPDPVALSSTYIYKTLVFVMTPVSWFTNKAAYGFLRLFGVK
                     TVNESGQAMSPEELRSVVAEAGPMIPTRHRQMLLSILDLEHVTVNDIMIPRQEIASIN
                     ADDNWEDILDQLRQTPHTRLPLYEGDLDNLVGIVHMKRVAQELARDTLSRERLIELAR
                     TREPYFVPEGTSLTVQLNQFQRNRRRMAFVVDEYGDIQGFVTLEDILEEIVGEFTTDP
                     ATVTHKHVHREAPGVFIINAGATVRAINRSLGWQLPTDGPKTLNGLLVEKMETLPEAG
                     DSTEIGDYQFEVLQIGENTIRTVRARRGGTAAAT"
     gene            complement(4075..>4736)
                     /locus_tag="VEW08_13945"
     CDS             complement(4075..>4736)
                     /locus_tag="VEW08_13945"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HYJ41890.1"
                     /translation="LYFESDASSRFRNVRATKNRCGSVGELAFFHMGEAGLREVRNPS
                     AIFLARSGVPAPGSIVAVTRDGRRPLLVELQALVDRMRFRSPRSVAQGLDAQRVAMLL
                     AVMNRHAGVSLQEHDVFVNLVGGLEVTETACDLPVALALASSLADKPIAQDLVAFGEL
                     GLTGELRPVAYGEERLREAAKQGFTTALIPKDNAPRAPIEGLRVREITRLDDALKAAL
                     G"
BASE COUNT          828 a         1623 c         1420 g          865 t
ORIGIN      
        1 ttctctatct atacgcgatt cgatgacccg ctcgtcgagt ccttcgtatc gccccgccac
       61 cagggtcagc cccggcagct tcgaaaattc ctgcgccagc gcctgcgtga agggcacgcc
      121 ctgcgccgac atatatatgc gcggcccgcc cggcaccctc gaggccgcgg cgtcgattgc
      181 cgccgccatc ggttccggct tcaacaccat gcccggtcca ccgccataag gccggtcgtc
      241 caccgtgcga tgcacgtcgc gggtgtgcgc gcgcgggtct tcgcaaccca ccgacacgat
      301 gccgcgctcc accgcccggc ccagcacacc caccttcagc gcctgctgaa tcaactccgg
      361 aaacaatgtc acgacttcga tgcgcatgag tcgtgtcaga aatcctcggg ccaatccacc
      421 agaaccgtgc gcgcatcctt gtcgatgctg cgcaggtgct gcccggtcac tggaacccag
      481 tgttcgcgcg cacccttgat caccatcacc gcgttgcccg gcgtatcgac gaaatggtcg
      541 actgcaccca gcaccgcacc ttccagattc ttcgcctcga aaccgatgag gtcatcgcga
      601 tagtgctcgc gtccctcggc cggtggcagc gcttcccgct ccacgagaat catctgcccg
      661 gtcaaccggc tcgcgccatc gcgttcgtcg atgccctcga gccgcgcgat ccagccattg
      721 ccctgcgcgc gcgcctctaa caacttcgct tcacgcgtgg ccccattcgc tgcctgcaat
      781 tgccaggtgg gccactcgaa cagtcgttgc ggagggtcgg taaacgactg cacccgcaac
      841 cagccgcgca cgccgtgcgc cgctcccaat ctgccgactt ctatgtggct cggtttcacc
      901 gcgcctcctc ggcagctcag cagatcagct gatcaggcgt tcgccttgcg gaacgtcgtc
      961 acgagattct tcacgcgatc ggactgctgc gcgcccttcg agagccaata gtcgatacgg
     1021 gcgatgtcga gcttgagctt ggtctcgttc ttcaccgctt tcgggttgaa gaaaccgacc
     1081 tgctccaggc ttttgccacc ctggcgcttg cggctgtcgg tcaccacgac gtgatagaac
     1141 ggcgtgttag tggcaccgcg gcgggtcaga cgaattgtca ccatgtcatt gaaccttcaa
     1201 gttgcgtagc cccggggcga aatacccgtc ccggccggcc aaaaaagagc gcggattcta
     1261 cgcaaaccgg tgatttggtg gaagcttttt ccggtgccgg gtaagcggtg ccgggccggg
     1321 aaaccgggaa aacgcagaaa taccgatagg aacggcgttt tcccggtttc ccggcccggc
     1381 accgctactg cgggaatccg gggggcatcc cgcccttgaa ggcctggagc atgcgtttca
     1441 tgcgcccacc ggccatgctt ttcatcattt tctgcatttc catgaactgc ttcatgagct
     1501 tattgacgtc ctgaacctgg acaccggccc cggcggcaat gcgccggcgg cgggagccgt
     1561 ccagaagccc cggattccgg cgctccttgc gggtcatgga attgatgatg gcgatctgac
     1621 ggcggacgtc cttgtcgccc ccatcggccg gcagggctgc gcccttcatg gccgcctggg
     1681 gcagtttgtc catgagcgcc ccgacgccac ccatcttctg cagctgctcg agctggctct
     1741 tgagatccac caggtcgaat cccttgccct tggcgacctt cgaagcgagc ttctgggcct
     1801 cctcgacatt gacctgcttt tgcacctgct cgacgagtgc gaccacgtcg cccataccga
     1861 gaatgcgccc cgccatccgt tcggcgtcga acggttcgag cgcgtcgagc ttctcgccga
     1921 cacccacgag aacaatgggt ttgcccgtga cctcgcgcac cgacagcgcg gcgccaccgc
     1981 gcgcatcgcc gtcggccttg gtgaggatga cgccggtgag atcgagcgcc gcgccgaacg
     2041 cgcgcgcgga attcaccgcg tcctggccag ccatggcgtc gatgacgaac agtcgctcgt
     2101 gggtttccac ggcgcgctcg atttcgcgca cttcttccat gagctcggcg tccacgtgca
     2161 ggcgtcccgc cgtgtcgacg atcagcgcgt cgtaaacgcc cttgcgcgcc gcatcgagcg
     2221 cgtcgcgcgc gatgtccgcg ggcttctgtt tgccgtcgct ggggaagaat ccggcgccca
     2281 cctgctccgc gagacgctgc agctgcagta tggctgcggg ccgtcggaca tccgtgctga
     2341 ctaacaagac gcgcttcttg tgggactcca tgagccagcg agcgagcttg ccggcggtcg
     2401 tcgtcttgcc ggcgccctgc aggcccgcta acaacacgac gacgggcggc tgcgcgcgca
     2461 gttcgaagcg cccccgatcg ccactcatga gcgcgatcag ttccttgtga aggatgccga
     2521 cgaacgcctg gccgggagac aggctggcag taacttcggc gcccagcgcc ttctgtttca
     2581 cccgctcgat gaaggccttg accactggca gcgcgacgtc ggcttcgagc agggccatgc
     2641 gcacttcgcg cagggactcg ccgacgttct cctcggtgat acggccgcgg ccgcgcagcg
     2701 tgtctacggt gcgggaaagc cgctgtgtga ggttctcgaa aagtgccata ggggcgggat
     2761 tatagaagcc ctatgctatg cggcatatgc aaaacgcacc ctcctccatc ctcggtatcg
     2821 cgcttgtagt cctgttacta ctgatcgcgt tttcctcggg cacggaagtg gccatgttgt
     2881 cggccaaccg ttacaagatc cgcgcggcgg cgaaggccgg caaacgcacc gcgaaagcgc
     2941 tcgacgcgct gctttcccgg ccggagagct ggctcggcgc caacctggtg ctgctcgcat
     3001 tggcgagcgt ggcggcctcg tccatcgcca cgctgctggc gcagcgcacg cgctacgagc
     3061 acgcgatccc ggtcacgatg gcggtgctct cgatcgtcat gatcgtgttc tgcgagctcg
     3121 cgccgaaaat ctacgcagcg ctgaagcccg acccggtggc gttgtcgtcc acctacatct
     3181 acaagacgct cgtgttcgtg atgacgccgg tgagctggtt caccaacaag gcggcgtacg
     3241 gcttcctccg gctcttcggc gtcaagaccg tcaatgaaag cggccaagcc atgtcgccgg
     3301 aggaattgcg ctcggtcgtt gcagaagccg gacccatgat tccgacgcgt caccggcaaa
     3361 tgctgctgtc gatcctcgat ctcgaacacg tgaccgtgaa cgacatcatg attccccgcc
     3421 aggagatcgc gtcgatcaac gccgacgata actgggagga catcctcgac cagcttcgcc
     3481 agacgccgca tacgcgcctg ccgctgtacg aaggtgatct cgacaacctg gtcggcatcg
     3541 tgcacatgaa gcgtgtcgct caggaactgg cgcgcgacac gctttcgcgc gagcggctga
     3601 tcgagcttgc gcgcacgcgc gaaccgtact tcgtgcccga aggcacctcg ctcacggtgc
     3661 agctcaacca gtttcagcgc aaccgtcgcc gcatggcgtt cgtggtcgac gagtacggcg
     3721 acatccaggg tttcgtcacg ctcgaggaca tcctcgagga gatcgtgggc gagttcacga
     3781 ccgatccggc cacggtgacg cacaagcacg tacatcgtga agcgcccggc gtgttcatca
     3841 tcaacgcggg cgccacggtt cgggcaatca atcgctcact gggctggcaa cttcccaccg
     3901 acggtccgaa gaccttgaat gggttgctcg tcgagaagat ggaaaccctg ccggaagccg
     3961 gcgacagcac cgaaatcggt gactaccagt tcgaagtgct gcagatcggc gaaaacacga
     4021 tccgaaccgt acgggcgcgc cgcggaggga ccgccgccgc aacctgagcc gccgtcaacc
     4081 cagcgcagct ttcagggcgt cgtccaggcg cgtgatctcg cgcactctca acccttcgat
     4141 cggagcgcgc ggtgcgttgt ccttcgggat gagcgcggtc gtgaaacctt gttttgccgc
     4201 ctcccgcagt cgctcttcgc cataggccac gggtcgcagt tcgcccgtca ggccgagttc
     4261 gccgaaagcc accaggtctt gcgcgatggg tttatcggcc aggctcgacg ccagcgccag
     4321 cgccaccggc agatcgcagg cggtttccgt gacctcgagc ccacccacca ggttcacgaa
     4381 cacgtcgtgt tcctgcagcg agactcctgc gtgccgattc atgaccgcca gcaacatggc
     4441 cacacgctgg gcatcgagcc cctgcgcgac gctacgcggc gatctgaaac gcatgcgatc
     4501 gacgagggcc tgcagttcca ccagcagcgg ccggcgccca tcgcgcgtga ccgcgacaat
     4561 gctgccgggc gcgggcacgc ccgagcgtgc caggaagatc gccgaaggat tgcgcacctc
     4621 gcgcaggcct gcttcgccca tatggaagaa cgccagttcg cccaccgatc cgcaccggtt
     4681 cttggtggcc cgcacattgc gaaaacggct ggatgcgtcg ctttcgaaat aaagca
//
LOCUS       DASQJS010000353         9205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_847861, whole genome shotgun sequence.
ACCESSION   DASQJS010000353 DASQJS010000000
VERSION     DASQJS010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847861"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="VEW08_13950"
     CDS             complement(<1..1563)
                     /locus_tag="VEW08_13950"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HYJ41891.1"
                     /translation="MSKDKAKVAKKFSSRMQGARAVWVAVGILALAFLVGVLAPAWYF
                     AGRLVENNTAVRAIGELQQQPSRMQAALNSIQDRLRARGFVRDSIEQLKRATQQFETS
                     TASLKSGGGVSIGGFGGDDDVQKTITEMTDAWKKYQPALAPVANFTSIPYSDSEREGT
                     QLNMDGRELQRSVTNAATQARAYTPVLEKNLAQIIAGLQSSSDSLATALRTVVLIGVG
                     LAGALAALVGYLSLARGKQAAAALAARQQTEDILRTVREGLFLLDADLKIGEIHSDAT
                     GSLFQRESIAGITFEDLLRNLVSAKTLAIATKFVKVLWSERTKENLIRSINPLNEVEV
                     ITGGDGKQDKGETRYLEFNFHRVRRDGAITHVLVSVSDVTARVALAAQLKGAQDNAQT
                     QMDAFLSILHVDPNQLSSFLDDSDVAFKMINATLRDPARDSASFRRKIDGLFRQIHSI
                     KGEASAIGLGSMEQRAHALESDLAALRERTDLTGDDFLPLLTKFDDLVSHSQSVREMV
                     TKLASFRDSFAPK"
     gene            complement(1579..3618)
                     /locus_tag="VEW08_13955"
     CDS             complement(1579..3618)
                     /locus_tag="VEW08_13955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYJ41892.1"
                     /translation="MQARSEIGATAQNDTTAKGSNMGASSFKSKRPLALIATPDLDLW
                     AQLGPMLESTCQLRHADSLSSAANMLKPGTRTILVADLRGMSPEDFAPISASPHNPVV
                     IAIRDAASAGMVDALMLEGEIQALVESPVESSHMLRAINDAARISSTADALSAVTTPG
                     ASSGGEAKSGKSPAVMIGVVVAVLAAAAGGWFFMSKGSSTQAPAAADSAATPAAGAPA
                     QAKAPAAAAAPAASLDEVLEKARLAMDERRYIEPAKNNALEHFRTVLQLDPGNAEATE
                     GLKRLGGLLIARARTALDERRFDNALAELEAARSIDPNDQRLVEVDARLDSMRAQTAL
                     AQIQATLAAQNFDRAQALLEAAQKDALLPPAQIAQLSKDLDNKRRSVNINRLASSAES
                     RLGSGRLVTPAGDSAKDAIQGLREAGASAELITRLNNDLNQRLLAAARDAAAKGDQAA
                     VAANLAAARENGVSAAVINAASRDITAAAQKQQRTNDDIARFAKAAQDRLASGNLIQP
                     ANDSAASNAEQLRKLDVRHPTTVQVVRDVRARLVSEARARLQGGRPTDANALLDAAES
                     LGGDADLADLRAAVATAQQAVATAAAAPKLPPLKMTRPPRPKYPASAKGVEGWVRVEF
                     YVTPEGRTERVRVLSSEPAGLFDAAAISAMEGARFEEFASTDSRLAVQRIVFKPE"
     gene            complement(3962..5221)
                     /gene="murA"
                     /locus_tag="VEW08_13960"
     CDS             complement(3962..5221)
                     /gene="murA"
                     /locus_tag="VEW08_13960"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HYJ41893.1"
                     /translation="MDKLQINGGIALEGEVRISGAKNATLPIIAAALLADGPVTIRNV
                     PHLQDVTTMIELLGRMGVSVTIDEKMRIEIDPTTTRECFAPYELVKTMRASILVLGPL
                     LARFGKADVSLPGGCAIGARPVNIHVAGLQAMGADIQIDSGYIRARAGRLKGARLNLD
                     TVTVTGTENLMMAATLADGETIIENAAREPEVVDLANFLISMGAKIQGAGTDKIVIQG
                     VEKLHATSYEVQPDRIEAGTYLVAGAITSGLVRVKNVNPEHLDAVIGKLREAGATVGV
                     GSNWVEVDMKGKRPKSVDVRTAPYPAFPTDMQAQFAALNTIADGVGTIVETIFENRFM
                     HMLEMRRLGAEIRLEGNTAIIRGVPKLTAAPVMATDLRASASLVLAGLVAEGRTEIER
                     IYHIDRGYEALEEKLAQIGAQIRRVPN"
     gene            complement(5233..5487)
                     /locus_tag="VEW08_13965"
     CDS             complement(5233..5487)
                     /locus_tag="VEW08_13965"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HYJ41894.1"
                     /translation="MNSSVMTPDRIADLIRAGIPGAAVRVESDDNTHFASQIVSAQFA
                     GLRPIARHQLVYQALGALMGREIHALSIEALTPEEFRARG"
     gene            5517..6503
                     /locus_tag="VEW08_13970"
     CDS             5517..6503
                     /locus_tag="VEW08_13970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HYJ41895.1"
                     /translation="MNAQLKTDPASLIAAGRRALEIEGRAIGALVPRLDASFARACAI
                     CLECHGRVIVSGMGKSGHIAGKIAATLASTGTPSFFMHAAEASHGDIGMITRDDALLA
                     ISNSGETGEVLLMIPHLSRLGVPIISMTGNPASSLARASTVHLDVSVAEEACPLNLAP
                     TASTTATLAIGDALAVALLEARGFTSQDFARSHPGGALGRKLLLHVADVMRTGEDLPV
                     IGPEKSLADGLVVMSKKGLGMCVVIEDGRLLGVFTDGDLRRALDRESNVHKTTMRDVM
                     TSPGKRVSATELAAEAAHLMEKHRITALPVVDAQGVLVGALNVHDLLRAGVL"
     gene            6500..7006
                     /locus_tag="VEW08_13975"
     CDS             6500..7006
                     /locus_tag="VEW08_13975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HYJ41896.1"
                     /translation="MSGAARDPAALARVRLLVLDVDGVLTDGRLFYGPKGELLKTFHV
                     RDGHGIKQVAAAGITVAIISGRKSAAVLRRARELGIRHVTQGATDKLAALRKLAKARG
                     VSMDECLCVGDDTPDAPILAAAGVGVAVADAHQDALAAADLVTTRAGGQGAVREVCDW
                     LIAARKAP"
     gene            7003..7617
                     /gene="lptC"
                     /locus_tag="VEW08_13980"
     CDS             7003..7617
                     /gene="lptC"
                     /locus_tag="VEW08_13980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HYJ41897.1"
                     /translation="MKNWSRTLKYVAAIAVIAVAYFIGTRGTDEDDTDAANATAPDPG
                     YAARDAQLIETGYDGRERYRLNASVIRQRTESGVIELENLSMNYHVESAGEAPGGPAS
                     AAPASADVWHLTSDHGEVRANGDDVQLTGNVRVVGPAPGTGEPLSLTTESVRINTPTQ
                     FIETSAAVKLRWSGHELDARGMQADLKAGTLRLESDVNGHFFPK"
     gene            7595..8266
                     /gene="lptA"
                     /locus_tag="VEW08_13985"
     CDS             7595..8266
                     /gene="lptA"
                     /locus_tag="VEW08_13985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HYJ41898.1"
                     /translation="MATSSRNSSALFRATTGALLAAGLLGVAEGAAAPCANQEIVLEG
                     KPLEMDYRNNNAVLRDVVITQCSVRIQAAVADIKGGLNFDNSHMTISGDVRITAEGGS
                     LSSDKAVVSFRNKLISAATITGTPAQFEQKREDGTTARGRANTIDYDTASGTVSFNTN
                     AFLSYGRNEATGQQFSYNIRTQSLQPGQVKPGANTGDGRVRFVIQPDKPLTMPDKDKD
                     KEKKP"
     gene            8263..8988
                     /gene="lptB"
                     /locus_tag="VEW08_13990"
     CDS             8263..8988
                     /gene="lptB"
                     /locus_tag="VEW08_13990"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HYJ41899.1"
                     /translation="MSVLRATHLAKSYKKRQVISDLSVEVESGQIVGLLGPNGAGKTT
                     AFYMIVGLVPPDRGTVTIDDRDITHLPMHQRARSGIGYLPQEASVFRKLTVEDNVMAI
                     LETRDNLDETARQERLEQILGELRIAHVRKSLGMALSGGERRRVEIARALAAEPRFVL
                     LDEPFAGVDPLSVIDIQRIMTELTAKGIGVLITDHNVRETLGVCGRAYIVSGGTVIAS
                     GSSEEILANPQVREVYLGQSFRM"
     gene            9057..>9205
                     /locus_tag="VEW08_13995"
     CDS             9057..>9205
                     /locus_tag="VEW08_13995"
                     /inference="COORDINATES: protein motif:HMM:NF012530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HYJ41900.1"
                     /translation="MKPSLQLKLGQTLTMTPQLQQAIRLLQMPALELQAHIREQLESN
                     VMLEPV"
BASE COUNT         1622 a         2977 c         2972 g         1634 t
ORIGIN      
        1 cttgggcgcg aagctgtcgc ggaagctcgc gagcttcgtg accatttcgc gaaccgactg
       61 cgaatgcgag accaggtcgt cgaacttggt gagcagcggc aggaagtcat cgcccgtgag
      121 atcggtgcgt tcacgcagcg cggcgagatc gctctcgagc gcgtgcgcac gctgttccat
      181 cgaacccagg ccgatggccg aagcctcgcc cttgatgctg tggatctggc ggaacagccc
      241 gtcgatcttg cggcggaacg aggcgctgtc gcgcgccgga tcccgcagcg tggcgttgat
      301 catcttgaac gcgacatccg agtcgtcgag gaacgagctg agctggttcg gatcgacgtg
      361 caggatcgag aggaacgcat ccatctgcgt ctgcgcgttg tcctgcgcgc ccttcaactg
      421 cgcggccagg gcgacgcgcg cggtcacgtc ggacaccgac accagcacgt gcgtgatggc
      481 accgtcgcga cgcacgcggt ggaagttgaa ctcgaggtag cgggtttcgc ccttgtcctg
      541 cttgccgtcg ccgcccgtga tcacttccac ttcattgagc gggttgatcg agcggatcag
      601 gttttccttg gtgcgctcac tccacagcac cttgacgaac ttggtggcaa tggccagcgt
      661 cttcgccgac accaggttgc gcagcaagtc ttcgaaggta atgccggcga tggattcgcg
      721 ttggaaaagc gagccggtcg cgtcggagtg aatctcacca atcttgaggt cggcgtcgag
      781 caggaacaga ccttcgcgca cggtgcgcag gatgtcttcc gtctgctggc gtgccgccag
      841 ggcggccgcc gcctgtttgc cgcgcgccag ggagaggtaa ccgacgagtg ccgccagcgc
      901 accagcgagg cccacgccga tgagcacgac cgtacgcagg gccgtggcca gcgagtcgga
      961 ggagctttgc aatccggcga tgatttgcgc gaggttcttc tcgagcaccg gggtgtaagc
     1021 gcgcgcctgc gtggcggcgt tcgtcaccga gcgctgcagt tcccggccgt ccatgttgag
     1081 ctgcgtgcct tcacgctcgc tgtccgaata cgggatcgag gtgaagttcg cgaccggcgc
     1141 gagggccggc tggtacttct tccaggcatc cgtcatttca gtgatggtct tctgcacgtc
     1201 atcgtcgccg ccgaagccgc cgatgctcac gccgccgccg gacttcagtg acgcggtgga
     1261 tgtttcaaat tgctgcgtgg cccgcttcag ctgctcgatg gaatcacgga cgaatccgcg
     1321 cgcgcgcaga cggtcctgga tcgagttgag tgccgcctgc atgcgcgagg gctgctgctg
     1381 cagttcgccg atggcgcgga ccgcggtgtt gttttccacg aggcgtccgg cgaagtacca
     1441 ggctggcgca agcacgccga ccaggaacgc cagagccagg atgcccaccg ccacccacac
     1501 agcgcgagcg ccctgcatgc gtgacgaaaa tttcttggcc acttttgctt tatctttgct
     1561 cacagaactc accctccatc actccggctt gaacacgatg cgctgaaccg cgagtcgcga
     1621 gtcggtcgac gcgaattcct caaaccgggc accttccatt gccgagatcg ccgccgcgtc
     1681 gaacaggccc gcgggctcgc tcgacaacac acgcacgcgc tccgtacggc cttcgggcgt
     1741 cacgtagaac tcgacacgca cccagccttc cacgcccttg gcggacgccg gatatttcgg
     1801 acgcggcgga cgcgtcatct tgagcggcgg tagtttggga gcggcagccg ccgtggcgac
     1861 tgcctgctgg gcagttgcga ccgcggcgcg cagatcggcg agatcggcgt caccgcccag
     1921 gctttcggcc gcatcgagca gcgcgttggc gtcggtgggc cggccgccct gcaggcgcgc
     1981 gcgggcttcg ctgacgaggc gcgcacgaac gtcgcgcacc acctgcacgg tcgtcgggtg
     2041 gcgcacatcg agtttgcgaa gctgctcggc gttggaagcg gcggaatcat tcgcaggctg
     2101 aatcagattg ccactcgcga gacgatcctg ggcagccttg gcgaagcgcg cgatgtcatc
     2161 gttcgtgcgc tgctgtttct gcgcggcagc ggtgatgtca cggctcgccg cgttgatcac
     2221 cgcagccgag acgccgtttt cgcgagccgc ggcaagattg gccgcaacgg cagcctggtc
     2281 acccttggcc gcagcgtcac gcgcggcagc aagcagccgc tggttcagat cgttgttcag
     2341 gcgcgtgatc aattcggccg aggcacccgc ctcgcgaagc ccctggatcg catccttggc
     2401 ggagtcgcca gccggggtca ccaggcggcc actgcccagg cgcgactcgg cgctcgacgc
     2461 cagtcggttg atgttgaccg agcggcgctt gttgtcgaga tccttggaaa gctgcgcgat
     2521 ctgggccggc ggcagcagtg catctttctg cgcggcctcg agcagcgctt gcgcgcggtc
     2581 gaagttctgc gcggcgagcg tcgcctggat ctgcgcaagc gcagtctggg cgcgcatgga
     2641 atcgagtcgg gcgtcgacct cgaccaggcg ctgatcgttc ggatcgatgc tgcgcgcggc
     2701 ttcgagctcg gcgagcgcat tgtcgaagcg gcgctcgtcg agcgcggtgc gggcgcgcgc
     2761 aatcagcagg ccgccgaggc gcttcaggcc ttcagtggct tcggcgttgc ccggatcgag
     2821 ctgcagcacc gtgcggaaat gttcgagagc attgttcttg gcaggctcga tgtagcggcg
     2881 ctcgtccatg gcgagccgcg ccttctcgag cacttcatcg agcgaggcgg ccggagcggc
     2941 cgcagccgcg ggagccttgg cctgagccgg agcgcccgcg gccggcgttg cggcggaatc
     3001 cgcagcggcg ggagcctggg tggaagagcc cttcgacatg aagaaccagc cgccagcggc
     3061 cgcggcgagt accgcgacca cgacgccgat catgacggcc ggcgatttgc cggatttcgc
     3121 ttcgccgccc gaactcgcgc ccggggtagt gaccgcgctc agcgcgtcgg ccgtggagga
     3181 gatacgcgcc gcgtcattga tcgcgcgcag catgtgactg gattcgaccg gagactcgac
     3241 cagcgcctga atttctcctt cgagcatcag ggcgtcgacc atgccggccg aggcggcatc
     3301 gcggatcgcg atgacgacgg ggttgtgcgg gctggcggaa attggggcga agtcttccgg
     3361 actcatgccg cgcaggtcgg caaccaggat ggtgcgcgtg cccggcttga gcatattggc
     3421 ggcggaactg aggctgtccg cgtgccgtaa ctggcaggtt gattcgagca tgggcccgag
     3481 ctgcgcccac aaatcgaggt caggcgtggc gatgagcgcc agcggacgtt tcgatttgaa
     3541 tgagctggct cccatattgc tgcctttggc tgtggtgtca ttttgcgcgg tcgcgccgat
     3601 ttccgaacgt gcttgcatgg accctcgacc tctcatcgga ctggttgcga cgatggctct
     3661 tcctcgcgtt gacggggcat tcttggcccg tctccgggga acgacagtga ggggcagtat
     3721 taagtcagtg cgttgccggt gatcggcact gagtcacatc cggcacacgg aaacccctca
     3781 agaacagccg gttcgcgacc tggatcacaa atggtgcatg gggacattcc cggtccccgg
     3841 cgcaaaaggg gacacacctc tcgaaagcgc tgtaagcctt taaatgcagt ataaaaccgc
     3901 atgccaggtg cgtcctcgtt tgccgaaacg aagaatgtcc cgattgtctc ccggagccct
     3961 cctagttagg tacgcgccgg atctgcgcgc cgatctgcgc cagtttttcc tcgagcgcct
     4021 cgtagccacg atcgatgtgg tagatgcgct cgatttccgt ccttccttcg gccaccaggc
     4081 ccgccaggac caggctggcc gaggcgcgca ggtctgtggc catgaccggc gccgcggtga
     4141 gcttgggcac gcccctgatg atggcggtat tgccctcgag gcgaatctcc gcacccagcc
     4201 ttcgcatctc gagcatgtgc atgaagcggt tctcgaaaat cgtctcgacg atcgtgccga
     4261 caccatcggc gatcgtattc agtgccgcga actgcgcctg catgtcggtg ggaaacgccg
     4321 gatacggcgc ggtgcgcaca tccaccgact tcggccgttt gcctttcatg tccacttcga
     4381 cccagttcga cccgacgcct accgtcgcgc ccgcttcgcg cagtttcccg atgacggcgt
     4441 ccaggtgttc gggattcacg ttcttgacgc gcaccagtcc cgaagtaatg gcaccggcca
     4501 cgagataggt acctgcctca atgcgatccg gctgcacctc gtagctcgtc gcgtgcagct
     4561 tctccacgcc ctggatcacg atcttgtcgg ttccggcgcc ctggattttc gcgcccatgg
     4621 agatcaggaa gttggcgagg tcgaccactt cgggttcgcg cgcggcgttt tcgatgatgg
     4681 tttcaccgtc ggcgagcgtc gccgccatca tgaggttttc ggtgccggtg acggtaacgg
     4741 tgtcgaggtt caggcgcgca cccttcagtc gcccggcgcg cgcgcggatg tatccgctat
     4801 cgatttgaat gtcggcaccc atcgcctgca ggccggcgac gtggatgttc accggccgcg
     4861 cgcctatggc acagccaccg ggcagcgaca cgtcggcctt gccgaaacgc gcgagcagtg
     4921 gcccgagcac caggatcgac gcgcgcatgg tcttcaccag ttcgtatggc gcgaaacact
     4981 cgcgtgtcgt cgtcggatcg atctcgatgc gcatcttctc gtcgatggtg accgagacgc
     5041 ccatgcggcc taacaactcg atcatggtgg tgacgtcctg caggtgcgga acgttgcgaa
     5101 tggtcaccgg gccatcggcg agcagggctg ccgcgatgat cggcaaagtc gcgttcttgg
     5161 cgccggaaat gcgcacctcg ccctcgaggg cgataccacc gttgatctgc agcttgtcca
     5221 aaaatcgtct ccctatccgc gtgcgcgaaa ttcctcgggc gtcagcgctt cgatggacag
     5281 ggcatggatt tccctgccca taagcgcgcc gagagcctgg tacacgagct ggtgtcgcgc
     5341 gatcggccgc aggcccgcga actgtgccga aacgatctgc gaagcaaaat gggtgttgtc
     5401 gtcggactcc actcgcaccg cagcgcctgg tattccggcc cggatgaggt cggcgatacg
     5461 gtccggggtc atgacactcg agttcatcgc gttatcttaa gcgttatgat cgacgcatga
     5521 atgcccagct aaagacggat ccggcctcgc tgatcgccgc cggacgccgg gcgctcgaga
     5581 tcgagggccg ggccatcggg gcgctggtgc cgcgcctcga cgcaagtttt gcgcgtgcct
     5641 gcgccatatg cctcgaatgc cacggccggg tcatcgtcag cggcatgggc aaatccggtc
     5701 acattgccgg gaaaatcgcg gccacgctgg cctccacggg cactccgtct ttcttcatgc
     5761 atgccgccga ggccagccat ggcgacatcg gcatgatcac gcgcgacgac gcgctgctgg
     5821 ccatatccaa ctccggcgaa accggcgaag tgctgctgat gatcccgcac ctgtctcgcc
     5881 tgggggtgcc catcatttcc atgaccggga atccggcgtc gtcactggcg cgcgcctcca
     5941 cggtccacct cgatgtgagc gtcgcggagg aagcctgccc gctcaacctc gcccccacgg
     6001 ccagcaccac agccacgctc gccatcggcg acgcgctggc ggtcgccctg ctcgaagcac
     6061 gcggcttcac ctcccaggat ttcgctcgct cccatcccgg tggcgcgctc ggccgcaagc
     6121 tgctgctgca cgttgccgat gtcatgcgca cgggtgaaga cctgccggtc atcggtccgg
     6181 agaaatcgct ggccgacgga ctcgtcgtga tgtcgaagaa gggcctgggg atgtgcgtcg
     6241 tcatcgaaga cgggcgcctg ctcggcgtgt tcaccgacgg cgacctgcgc cgcgcgctcg
     6301 accgcgaatc caatgtccac aagacgacga tgcgcgatgt catgacttcg cccggcaagc
     6361 gtgtaagtgc cacggaactg gctgccgaag cggcccatct catggagaag caccgcatca
     6421 ccgcgcttcc ggtggtcgat gcacagggcg tgctggtcgg tgcgctcaac gtgcacgacc
     6481 tgttgcgcgc cggagtgctg tgagcggcgc agcgcgggac ccggctgcgc tcgcgcgcgt
     6541 gcgcctgctg gtgctggatg tggatggagt actcaccgac gggcggcttt tctacggccc
     6601 gaagggcgag ctgctgaaga ctttccatgt gcgcgacggt cacggcatca aacaagtcgc
     6661 agcggctggc atcaccgtcg ccatcatctc cgggcgcaaa tccgccgcgg tgctccgccg
     6721 cgcaagagaa ctgggcattc gccacgtcac gcagggcgcc accgacaaac tggcggcgct
     6781 gcgcaaactc gcaaaagccc gcggcgtgtc gatggacgaa tgcctgtgcg tgggtgacga
     6841 cacgccggac gcgccgatcc tcgccgccgc aggtgtgggc gtcgccgtgg ccgatgcgca
     6901 ccaggacgcg ctcgcggctg cggacctggt caccacgcgg gccggcggac aaggcgcggt
     6961 ccgcgaggtc tgcgactggc tgatcgcggc gcgcaaagcc ccatgaaaaa ctggtcccgc
     7021 acattgaagt acgtggcggc catcgccgtg atcgcggtgg cctatttcat cggtacccgg
     7081 ggaaccgacg aggacgacac ggatgccgcg aacgcgaccg cgcccgatcc cgggtacgcc
     7141 gcgcgcgacg cacagctcat cgaaaccggc tacgacggcc gcgaacgcta tcggctcaac
     7201 gcgagcgtca tccggcagcg caccgaatcc ggggtcatcg aactcgagaa cttatcgatg
     7261 aattatcacg tcgaatccgc aggcgaggca ccgggagggc cggcgtccgc cgctcctgcg
     7321 tcagccgatg tctggcacct cacgtcagat cacggcgagg tgcgcgccaa tggcgatgac
     7381 gtccagctga ccggaaacgt tcgtgtcgtc ggcccggcgc cgggcaccgg cgaaccgttg
     7441 tccctgacca ctgaaagcgt gcgcatcaat actcctacac agttcatcga gaccagcgca
     7501 gcggtcaagc tgcgatggtc aggacatgaa ctcgatgcac gcggaatgca ggccgatttg
     7561 aaggccggca cgctgcgtct agaatcggat gtgaatggcc acttcttccc gaaatagctc
     7621 cgccctcttc cgggcgacca cgggcgccct gctcgccgcg ggattgttag gcgtggccga
     7681 gggcgccgca gcgccatgcg ccaatcagga aatcgtgctc gaaggaaagc cgctcgaaat
     7741 ggactaccgc aacaacaacg cggtgctgcg cgacgtggtg atcacccaat gcagcgtacg
     7801 catccaggcg gccgtggcgg acatcaaggg cgggctcaat ttcgataaca gccacatgac
     7861 catttccggt gacgtgcgca tcacggccga aggcggcagc ctgagctccg acaaggccgt
     7921 cgtctcgttt cgcaacaagc tcatatccgc ggccaccatc acgggcacgc ccgcacagtt
     7981 cgagcagaag cgcgaggatg gcactactgc gcgcggccgc gccaacacca tcgactacga
     8041 tactgcgagc ggcaccgtga gcttcaacac caacgccttt ctgtcttatg gccgcaatga
     8101 agccaccggc cagcaattct cctacaacat ccgcacgcag agcttgcagc cgggacaggt
     8161 gaaacccggc gccaacaccg gcgacggccg ggtgcgcttc gtgatccagc cggacaaacc
     8221 actgacgatg cccgacaaag ataaagacaa agagaagaaa ccgtgagcgt gcttcgggcc
     8281 actcatctcg ccaaaagtta caagaagcgc caggtgatct ccgatctctc cgtcgaagtg
     8341 gagagcgggc agatcgtcgg cttgttaggc ccgaatggcg ccggcaagac cacggcgttc
     8401 tacatgatcg tcggcctggt gccgcccgac cggggtacgg tgaccatcga cgaccgcgac
     8461 atcacgcatc tcccgatgca ccagcgcgcg cgttcgggca tcggctacct gccgcaggaa
     8521 gcttcggttt tccgcaagtt gacagtcgaa gacaacgtca tggcgatact cgagacgcgt
     8581 gacaacctcg acgaaaccgc ccggcaggag cgcctggagc agatcctcgg ggaactacgt
     8641 atcgcgcacg tccgcaagag cctggggatg gcgctgtcag gcggcgaacg ccggcgcgtc
     8701 gaaatcgcgc gtgcgctggc cgcggagccc cgttttgtgc tcctcgatga accgtttgcg
     8761 ggcgtcgacc cactttcggt catcgacatc cagcggatca tgacggagct caccgccaag
     8821 ggcatcggcg tgctcatcac cgaccataac gtcagagaaa ccctaggtgt atgtgggcgc
     8881 gcatacattg tttccggagg aaccgttatc gcttcaggtt cttcggaaga aatccttgcc
     8941 aacccccaag tccgtgaggt gtacttgggc cagtctttcc gtatgtagcg ttccactgcg
     9001 gacgcggaaa aaactatttc tttcatagcc ttgagacact cacgcccgtg tcgatgatga
     9061 agccatctct gcagctgaag ctgggtcaga cgctaacgat gaccccgcag ctgcagcagg
     9121 ccatccgtct cctgcaaatg cctgcgctgg agctgcaggc gcacatccgc gaacagctcg
     9181 aaagcaacgt catgctcgag cccgt
//
LOCUS       DASQJS010000354         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3672441, whole genome shotgun sequence.
ACCESSION   DASQJS010000354 DASQJS010000000
VERSION     DASQJS010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3672441"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VEW08_14000"
     CDS             <1..774
                     /locus_tag="VEW08_14000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HYJ41901.1"
                     /translation="VGGTSSGEGYAATLQRLKPGIECDIVRPADEEPKLPEGMALEHY
                     HGAVITGSALNIYSREAAVERQIDLAKAVFAAGVPCFGSCWGLQVGVTAAGGSVVRNP
                     RGREFGFGRRITLTSQGRDHPMFLGKPEVFEAATVHVDTVESLPAGATPLAHNDMGLQ
                     AAEIKLKKGSFWGVQYHPEYSCAEIAAMARRYADVLIRDGLVKDQAELDVLAADLVAL
                     DKHPDDARLAWRFGVGPSITDPGIKLAELRNWLDRQVLK"
     gene            838..1863
                     /locus_tag="VEW08_14005"
     CDS             838..1863
                     /locus_tag="VEW08_14005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYJ41902.1"
                     /translation="MRILIVTDAWEPQVNGVVRTLKTTARELTKMNHEVRFLTPLDFR
                     TLPCPTYPEIRLSLFPQGGVNRLIREFDPDVLHIATEGPLGLAARRFALRNEFPFTTA
                     YHTRFPEYVHARFRLPLSWSYAWLRHFHGPSKGVMAPTQVVVDDLKANGFTNTRLWSR
                     GVDTDVFHPQPSKRLDSTPPIFLYVGRVAVEKNVEAFLELDLPGSKWVVGTGPALERI
                     RTRYPRVNYLGLLDREELAKVYAAADVFVFPSRTDTFGLVLLEAMACGLPVAAYPVTG
                     PRDVIGDSKAGVLHEDLRTACLGALKLDRADALARAGLFTWRAATEQFLGHLHPRKRT
                     SAMPAYA"
BASE COUNT          394 a          684 c          710 g          430 t
ORIGIN      
        1 gtcggcggca cgtcttcagg cgaaggctac gccgccacgc tccagcgact gaaacccggc
       61 atcgagtgcg acatcgtgcg tccggccgat gaagaaccga aactgcccga gggcatggcg
      121 ctggagcact atcacggcgc cgtcatcacc ggatcggcgc tgaacatcta ctcacgggaa
      181 gcggccgtgg aacgccagat cgatctcgcg aaggcggttt tcgccgctgg cgtgccatgc
      241 ttcggcagct gctggggact gcaggtgggt gtcaccgcgg ctggcggcag cgtggtgcgc
      301 aatccacgtg gccgcgagtt tggattcggc cggcgcatca cgctcacgag ccaagggcgg
      361 gatcacccca tgtttctcgg caaacccgag gtgttcgaag ccgcgaccgt gcacgtggac
      421 accgtggagt ccttgccggc cggcgccacg ccgctggctc acaacgacat gggcttgcag
      481 gcagccgaga tcaaactcaa gaagggtagt ttctggggtg ttcaatatca ccccgagtac
      541 agttgcgcgg aaatcgcggc gatggcgcgg cgctacgccg acgtgctgat tcgtgacggt
      601 ctcgtgaagg accaggcgga gctcgatgtg ctggccgcgg acctcgtggc gttggacaaa
      661 catcccgacg acgctcgtct cgcgtggcgc ttcggtgtgg gtccttcgat caccgatccg
      721 ggcatcaagc tcgccgagct gcgcaactgg ctggaccggc aggtgttgaa gtagccactg
      781 tcattccact gcaacgattt cgcggtggac tatgcctcaa ctggttagga ggcatatttg
      841 agaatcctca ttgtcacaga tgcctgggag ccccaggtca acggcgtggt gcgcacgctc
      901 aagaccaccg cccgtgaact gacgaagatg aatcacgagg tgcggttcct cacgccgctc
      961 gacttccgca cgctgccttg tccgacctat cccgagatcc gcctgtcact gtttccgcaa
     1021 ggcggtgtaa accggctgat tcgcgagttc gaccccgacg tgttgcacat cgccaccgaa
     1081 ggtccgttgg ggctggcggc gcggcgcttc gcactgcgca acgaatttcc gtttaccacc
     1141 gcctatcaca cgcgcttccc cgaatacgtg cacgcgcggt tccggctgcc gctgtcgtgg
     1201 agctatgcgt ggctgcggca cttccacggg ccttcgaagg gcgtgatggc gcccactcag
     1261 gtggtggtcg atgacctcaa ggccaatggt ttcaccaaca cgaggctctg gtcgcgcggt
     1321 gtggacaccg acgtgtttca tccgcagccg agcaagcggc tcgacagcac gccaccgatt
     1381 ttcctctacg tcggccgcgt cgcggtcgag aagaacgtcg aggccttcct cgaactcgat
     1441 ctgcccggat ccaaatgggt ggtgggtacg gggccggcgc tcgagcggat ccggacgcgt
     1501 tatccgcgcg tgaactacct gggtttgctg gatcgcgaag aactggcgaa ggtctatgcc
     1561 gctgccgacg tgttcgtgtt cccgagcagg accgacacct ttggtctcgt gttgttagag
     1621 gccatggcct gcggtctgcc cgtggcggca tatccggtca ccggtccgcg ggacgtgatc
     1681 ggcgattcga aggcaggagt gctccacgag gatctgcgca ccgcgtgcct gggcgccctg
     1741 aaactcgatc gcgccgatgc gctcgcgagg gctgggctgt tcacctggcg cgcggccacc
     1801 gagcaattcc tgggccacct gcacccgcgc aagcgcacct cggcgatgcc cgcatatgcg
     1861 tgattcctga gggcaaacaa tcagttgcgt tccttgcgtt gtgattgcgc gcgaagttct
     1921 cccggaaagc ttcgccgctg ctgaaagcgt ttcacttgcg ggccatccga gcgctcggcc
     1981 tatactcggt cccgaaaaag gggacagatc tatttttctg ttcccaggca actagaacaa
     2041 taagcaaggg gcagaaaaat agatctgtcc cctttttcgg ggagtcttta cgcatgaacg
     2101 agcaatcatc gtccggcggc gtcagctata agcaggtcgg cgcggactat ttcgagaagc
     2161 gcggtctcaa gcgctacgcc aaggtgtggt cgttgtgggc tctcggcgtc ggcgccgt
//
LOCUS       DASQJS010000355         3617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3366236, whole genome shotgun sequence.
ACCESSION   DASQJS010000355 DASQJS010000000
VERSION     DASQJS010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3366236"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VEW08_14010"
     CDS             <1..979
                     /locus_tag="VEW08_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41903.1"
                     /translation="SVRAVKALPSDLNNQYVPDAKVRYVLRDQAGPVWLRGKRSAVVN
                     TDDKSTYVFFGKLQPIETPAMVGFVDPQLPEWIRGSLAQSIPDILARYTQELGAPPGG
                     KPTIMLSWAGPTPGLTSRAGSVLPGLIVLTYEGSGVLSEDEGARVEGLQFIAHEAAHF
                     WLGQTVAYEYARDAWITEGGADLLAMRLTALEHAAYDWRDDFNNSIEECVNLTKRRGI
                     ESARDRNENRAYYACGAVFGLIAESASGKSFFRFVKNLVDANRVDGIVSRSEWLAALD
                     AASRKPDLSRDIARMLDRGVPDPKTFIAELFTRAGVVHSIDAEGMPRLP"
     gene            1112..1396
                     /locus_tag="VEW08_14015"
     CDS             1112..1396
                     /locus_tag="VEW08_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41904.1"
                     /translation="MHSRCKSCLYCGLVNYMTLGDPPPVTRWKRPVLLVAGIALAVST
                     VLALRFAWERPDGMFFASVSALLNIIALLGVAIAVRGCDACVARYLGKTL"
     gene            1663..1815
                     /locus_tag="VEW08_14020"
     CDS             1663..1815
                     /locus_tag="VEW08_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41905.1"
                     /translation="MPDTAARKNLLGEFKRVLRPGTPTKYATELNGLTMNGNPVRILQ
                     LWARST"
     gene            1855..>3617
                     /locus_tag="VEW08_14025"
     CDS             1855..>3617
                     /locus_tag="VEW08_14025"
                     /inference="COORDINATES: protein motif:HMM:NF013199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HYJ41906.1"
                     /translation="MSLTVVVAHAAGPAAGDWPNYGRTPGGDRHSPLAQIDRANVGKL
                     TLAWEYKTGEVEIKTGNPTALEATPLVIDGVMYLSTPLGKVIALDPVTGKQRWVRDVG
                     VKRDRYFGDWVSRGVSYWQDSRAKEGAVCARRIFFGVVDARMVALDATSGEFCRDFGT
                     GGEINLVPLLRNKQSYGDEYEQTSPPAVIGDLLVVGSAIADNNSTVGATGEVRAFDTR
                     TGALRWTWNPVPQDPADPAYKTWAGSAAHRTGGANTWSIIAADPKRDLIFLPTSSPNV
                     DYYGVTRLGDNRYANSVVALRASTGKIVWHFQTVHHDLWDYDNASPPALITLPDGRAA
                     VLQGTKTAQLFVLDRETGAPLFPVEERAVPKSDIPGEVTSPTQPLSSMSTGFRTLTAA
                     DIWGATPEDLAECRARFATLRYDGPFTPPSERGSVQVPANIGGAHWGGVAYDSDRGIA
                     IVPNNRLAAVVKLIPRETWKVMREKLTSGERVGKEFTDMQGTPYVMQREIWLSSKRSP
                     CTAPPFGTLTAFSLKTGKTLWDVPLGTSEGLDTIGIPSLPAGTGMANLGGAITTAGGV
                     VFIGSTLDAYVRAFDVETGREL"
BASE COUNT          650 a         1196 c         1147 g          624 t
ORIGIN      
        1 gtcggttcgc gcggtaaagg cgttgcccag cgatctgaat aaccagtatg tgccggacgc
       61 gaaggtccgg tacgtgctgc gcgaccaggc gggtccggtg tggctgcgcg gcaagcgctc
      121 ggcagtcgtg aataccgatg acaaaagcac ctacgtattt ttcggaaagc tgcagcccat
      181 agaaactccg gccatggtcg gtttcgtcga tccgcaattg cccgaatgga tccgcggctc
      241 actcgcgcag tccatccccg acattctggc gcgctacacg caggagcttg gtgcgccgcc
      301 cggcggcaag cccaccatca tgctgagttg ggccggcccg acaccggggc tcacgagccg
      361 cgcgggcagc gtgctgcccg ggctcatcgt cctcacctac gagggctcgg gagtgctctc
      421 ggaagacgaa ggcgcgcgcg tcgagggcct tcagttcatt gcccacgaag cagcgcattt
      481 ctggctcggg cagacggttg cctatgaata cgcgcgcgac gcctggatca ccgaaggcgg
      541 cgcggattta ttggccatgc ggctcacggc actcgagcat gccgcttacg actggcgcga
      601 cgacttcaac aattcgatcg aggagtgcgt gaacctcacg aagcggcgcg gcatcgagtc
      661 cgcgcgcgac cgcaatgaga atcgcgcgta ctacgcctgc ggcgccgttt tcggactgat
      721 cgccgaaagc gcgtccggca aatccttctt cagattcgtg aagaacctcg tcgacgccaa
      781 ccgcgtggac ggcatcgtgt cgcgctccga gtggctggcg gcgctcgacg ccgcgtcgcg
      841 gaagcccgac ctgagccgcg acatcgcgcg gatgctggac cggggagtgc ccgatccgaa
      901 aacgttcatc gcggagctgt tcacgcgcgc cggcgttgtg cacagcattg acgccgaggg
      961 catgccacgc ctgccctgaa ccgctcctct catctaaacc cctcccaaga tggcgcacga
     1021 acgagaaccg gaaacgcctg ccgtctgtgc tcctacagaa aactgtgcac gaattccccc
     1081 cctgcaacga gtgcagtggc aaattgacgc catgcattcg cgttgcaaat cctgcctgta
     1141 ttgcggtctc gtcaactaca tgacgctcgg cgacccgccg cccgtcaccc gctggaagcg
     1201 gccggtgttg ctcgtggccg gcattgcgct ggcggtctcg accgtgctcg cgttgcgatt
     1261 cgcctgggaa cggcctgacg gcatgttctt cgcgtccgtg tcggcactgc tcaacatcat
     1321 cgcgttgctc ggcgtcgcga tcgcggttcg cggctgcgat gcgtgcgtgg cgcgctacct
     1381 cgggaagact ctgtgaccaa gcgcccgacc gccgtctgca aggccaagag ccggcgcaag
     1441 gtgaagaaga agggtccgct tggtttgcgg ccgccacctc gatctgcctg ccgctacgcc
     1501 tccatctggc agtgcggagg ctacggcgag cgctcacggc gtatggcctc tgttgcccgc
     1561 tgactcggtt cgctgattca atgcgcttcc cgtggaagcg ccaaccaatc ttgaattctg
     1621 gaatcgcgcg gcgaccaccc gccagttcac gcacacgctc gcatgcccga tacagcagcc
     1681 cggaagaatc tgctgggcga attcaaacgc gtgcttcgtc ccggcactcc aacgaagtac
     1741 gcgaccgagc tcaatgggct tacgatgaac ggcaacccgg tgcgcatcct gcagctctgg
     1801 gcgcgatcaa cctgatcgga gagtcggatg aagagatttc tggttggggt cttgatgtca
     1861 ttgacggtgg tcgtcgccca cgccgccggg ccggccgccg gcgactggcc taactacggc
     1921 cgcacgccgg gtggcgaccg gcattcgccg ctcgcgcaga tcgatcgcgc caacgtgggc
     1981 aaactcacgc tcgcctggga atacaagacc ggcgaagtcg agatcaagac ggggaatccg
     2041 accgcgctcg aggctacccc gctcgtcatc gacggcgtga tgtatctgtc cacgccgctc
     2101 ggcaaggtca tcgcgctcga tccggtaacg ggcaagcaac gctgggtgcg cgacgttggc
     2161 gtgaagcgcg accgctactt cggtgactgg gtcagccgcg gtgtctcgta ctggcaggat
     2221 tcgcgcgcaa aagagggggc ggtgtgcgcc cggcgcatat tcttcggcgt cgtcgacgcg
     2281 cgcatggtgg cgctggatgc gaccagtggg gagttctgcc gggatttcgg cacgggcggc
     2341 gaaatcaacc tcgtgccgct gctgcgtaac aaacaatcct atggcgacga atacgagcag
     2401 acctcgccgc ccgcggtgat tggagatctt ctcgtcgtcg gttccgcgat tgcggacaac
     2461 aactccaccg tcggcgcgac tggcgaagtc cgcgcattcg atacgcgaac cggcgcattg
     2521 cgctggacct ggaatcccgt gccacaagat cccgccgatc cggcgtacaa gacgtgggcg
     2581 ggttcggcag cgcatcgcac gggaggcgca aacacctggt ccatcattgc ggccgatccc
     2641 aagcgcgacc tcatcttcct gcctacctcg agccccaatg tcgactacta cggcgtcacg
     2701 cgcctcggcg acaaccgcta cgccaactcc gtggtcgcgc tgcgcgcgtc gacgggaaaa
     2761 atcgtctggc atttccagac cgtgcaccac gatctgtggg actacgacaa tgcctcgcca
     2821 ccggcgctca tcacgttgcc ggacggtcgg gccgccgtgc tgcaggggac caagacggcc
     2881 cagttgttcg tgctcgatcg cgagaccggc gcaccgttgt ttcccgtcga agaacgcgcc
     2941 gtgcccaaaa gcgacatccc gggcgaggtc acttcaccca cacagccact gtcgtcgatg
     3001 agcacggggt tccgtaccct gacggcggcc gatatctggg gcgcaactcc cgaagatctc
     3061 gcggaatgcc gcgcacgatt cgcaaccctg cgctacgacg gacctttcac gccgccttcc
     3121 gaacgcggct cggtgcaggt gccggccaac atcggcggcg cgcactgggg cggcgttgcc
     3181 tacgacagcg accgcggcat cgccatcgtg cctaacaacc gcctggccgc ggtggtcaag
     3241 ctgattccgc gcgagacctg gaaggtgatg cgcgagaagc tgaccagtgg cgagcgcgtc
     3301 ggcaaggaat tcaccgacat gcagggcacg ccctacgtca tgcagcggga gatctggctg
     3361 tcgagcaaac ggtcgccgtg cacggcgccg ccgttcggca cgctcaccgc cttcagcctc
     3421 aagaccggca agacgctatg ggacgtaccg ctgggtacca gcgaaggtct cgacacgatc
     3481 ggcattcctt cgttgccggc cggcacgggc atggccaacc tgggcggcgc catcaccact
     3541 gcaggtgggg tcgttttcat cggctcaacc ctggacgcgt atgtccgcgc gttcgatgtc
     3601 gagacgggca gggaact
//
LOCUS       DASQJS010000356        12810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2419596, whole genome shotgun sequence.
ACCESSION   DASQJS010000356 DASQJS010000000
VERSION     DASQJS010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12810
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2419596"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1445)
                     /locus_tag="VEW08_14030"
     CDS             complement(180..1445)
                     /locus_tag="VEW08_14030"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HYJ41907.1"
                     /translation="MNLTYRALWRWHFHAGMFCIPFVVVLALTGSIYLFKPQIDAFAD
                     RGVDSLKVTGARAPGEAHIAAAIASVPGSKLFVYEIPREPDDAVRVHLYSPEGTGLIV
                     YVHPETLAVLKSVPHTHRLTEIVRMIHAELLAGRPGAILIELAASWAIVMLGTGLYLW
                     WPRDVRGIAGVLYPRASAGRRVFWRDLHAVTGIWVSAFAMFLLITALPWTTVWGAGLS
                     KTRALLTPEKQEWALSTVDEHTQHRAEAAAESITTPVSLDDIAKRVSPLNLDPPVRVY
                     LPNEHTPFWRVRAETQNRPRVRELELDARSGEVLRDHGFGEKAAIDRVIGVGIAAHEG
                     QLFGLANQLLGLFTALGLITMCVSAVVMWWRRRPEGSLGIPASRVEGFRIHAPLIALI
                     VCAGALLPVLGASLLILWASTRAEVFARP"
     gene            1563..2435
                     /locus_tag="VEW08_14035"
     CDS             1563..2435
                     /locus_tag="VEW08_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYJ41908.1"
                     /translation="MPRRLRIHVPGGFYHVTLRGNHQQDIFRSDGDRQLLNAIVARAI
                     EAYGARIHAYCWMSNHLHFLLQVGDQPLGNVVRQIASGYARAFQQKLPTTGHLFERRH
                     HASLVDVDSYLLELLRYIHLNPVRANLVSRPAEYRWSSHLAYAGRKAAEPWLTTSFAL
                     AMFSEDVRSAHAAYGRFMECPSTFGLEESLPSIKAISTEPRPLDQVPSASIAVIPRQT
                     LAQIIDEGCRRFGVLEQDLPTASRDPLLVQARAWIAREALERRVSTLSAVARALGRDR
                     ATLRFGIRLRRSSG"
     gene            2539..2613
                     /locus_tag="VEW08_14040"
     tRNA            2539..2613
                     /locus_tag="VEW08_14040"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2571..2573,aa:Val,seq:gac)
     gene            2800..4551
                     /locus_tag="VEW08_14045"
     CDS             2800..4551
                     /locus_tag="VEW08_14045"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HYJ41909.1"
                     /translation="MRAIPLTIVLLFGSAVAAAAGVKIEINGLPEEQREAVRASLELN
                     NYDKRDISPAELRAAYRKADEQIKSALEPFGFYDPRITPHLTGDEANGWTARFDVVPG
                     DPAIVRVAKVEVAGEGKEQRRVAEAVSAFVPKVGQRFDHATYEASKAVVDTSLRGAGF
                     LDAKYLTRRVTVRPEEQSADIDLTWESGIRYRFGAVRFSGDAPFPEEFLREFVSWDEG
                     AYFNSEQVLNLQQRLVDADYFELVSVQPALDEKKDGTVPLDILLKRDERTVYSGEVYY
                     STDFGGGVRLGADRRWLNRKGHKAGAKVEYSERLQEAEVHYKIPLPGREDRTYNFGVG
                     YRDETTDVNRSQNFQIAATRSEKRWHGFTRTLGLKYLVGDFEIGQDEDNLEYGNSNLL
                     FAEGTLSRRRINDRLAPRKGYVLEFGGRLAAEALVSDTDLAQVFGRITWLIPQGERGR
                     FKLRGEAGAMSVGNFDALPPDLRFYAGGDRSLRGFDYHDIGEVSANGNIIGGEFLAIA
                     SAEYEYYFTEDWGAGVFVDAGDAFTNGFSLNVGAGLGIRWRSPLGPIRVDVGFPVQSD
                     LPTATSWRLHVLLGPDL"
     gene            4548..7916
                     /locus_tag="VEW08_14050"
     CDS             4548..7916
                     /locus_tag="VEW08_14050"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HYJ41910.1"
                     /translation="MKAPPFKKILLWSGGSLALLLLLVAALLAWLLFTTPGARWVAST
                     VTLRFAPQVKYARIDGTIAGELTVTDLQFDGGADKARIRIQSITVDPTLMMLFSRALR
                     IDNARVSGLTLILPPEKDEPEPEEPLWIEPPLDLTVNDFLLTDATIYRENEKLATIRR
                     AELSARWKSRELIIETLTVKPGDIEGDFVVSGRIAPEGDTVRLALKAQWKEVVVPENL
                     AGRVLASHGGIDIEGTPKAYAVKGALDIGPPDELVHVLIDASGTDKRADLRQLELRQS
                     AGHLALSGTVEFKPVAWSLYAKASDFNPGALFAGWPGRLTIDASTRGLLAEAGPRGDL
                     QIATLSGTLRGRPIAGEGEIEFEAPSKFAGDLRVSSGKSRVTVKGSSGDRQQIDATVD
                     LAVASLNDWVPDTRGSLAGRFTIRGEWPKLTIAGSADGKSLGMGENEIAKLHVDATVA
                     SPLDPDGELQAVATQITLGGRMLAKVTLDATGNQAKHRVAVSADSEQYDGTVELAGGL
                     TKRGWSGELTRLQLEAVDIGTLTLREPSRVVYDAGNFSLSQTCLEDLPTVLCSAMKFE
                     ASGALDAGYSFERVPLKLANALAPDALAGELQGDVQGHGRVRRDANGQWIGDLAVTSE
                     SARMVLAQGEDQSESAAAATKLATEGSLLIYENLGLQADFAGMKANAKLSAKLAHGGY
                     LNVSMTVSDLNAPAPRLAGLIQATMPTLAPFGVFVPAVANLDGAVNAEIEVGGTTAKP
                     EFTGNVDATKLQADLGALGIKLRDGRLLGEARRDGGFKLAGSVASGKGHIELDGAMDE
                     RGVIEAKIIGQNFLAADIPAANVVVTPDLTLTGDPKGYLLRGEVNIPSAVINLQKFPQ
                     DQAPGVSPDVVVIRNGKEVVDEERAKSLPLTAVVTVNLGDDIKVTGYGLDATVTGQLT
                     VREAPDTPTTGSGQVSVAGRYEAYGQKLNIEDGRLLFAGTPLDNPRLAITAMRKVDEN
                     LSTGVRIAGTAKRPIITVVSSPEVGEADALSYLVTGRSLSEVGSASGSSQDALNSARQ
                     SLSGAGAGLVAKRIGARLGLDEAGVEENDLIGGSALTIGEYLSPRLYLSYGVGLFEPG
                     EVIALRYKLTEDVGVRIQRGTEETRTGVEYRIEK"
     gene            complement(7963..10560)
                     /gene="ligD"
                     /locus_tag="VEW08_14055"
     CDS             complement(7963..10560)
                     /gene="ligD"
                     /locus_tag="VEW08_14055"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HYJ41911.1"
                     /translation="MADAGNRSPTRLKRYVAKRDFRKTAEPSHKAPGARNKKVIFVVQ
                     KHAARRLHWDFRLEWAGTLRSWAVPKGPSLDPADKRLAVEVEDHPLAYAKFEGDIPKG
                     QYGGGHVDIWDNGTWEPVGDFAKGLKKGHLEFELFGKKLSGRWHLVRTRMQGKQPNWL
                     LMKSGDFAARVGADADIIDAGKADDPKRMLKRRTAARPAFVPKPKPGGVPKVRSSRSK
                     GARGHALPASLKPMLATLVDKVPGDEGWVYELKYDGVRLLSRCDGDDVRCISRNGIDW
                     TRKVDPVVQALAKMKLDGAWVDGELIVTDPNGRSDFSLLQHSMEQGRLDELQLCIFDL
                     LYYRGEDLRGLPLSERKTRLDAAFAKLPAKGPLRLADQIHSDSAEMLTRVCNQHLEGL
                     VAKKIDAPYIGDRSANWLKVKCHREQEFVVGGAAFLPGRGTGTFSSLLVGVKSGKGLK
                     YVGRVGGGFDANERAEWHERSKKLAQKSSPFDNHPEKRSGEIWHWMKPELVIQVAFAD
                     WTHGGILRQPRYLGMRQDRDPKTVVREEPEHTVDVVKETKRAKAKGQGKSTSRAAAKT
                     KVPASLGRLTHPDRVLFPHDGITKLQLAEYFAAIGEQAMPHYKDRPLSILRNTHGSKP
                     FFQKHFLEKSGDGLRVVKIPNADKDPDFTVCESPEGLLHLAQVGAVELHSWGAVMPRP
                     RLADRLTFDLDPGADLPYPPLRDAALAIRKLMKDLGLESWVKTTGGKGLHVVVPLGAP
                     RPDWDTAKGFALGVTRFMEKLAPTMFTSKTGERNRKNKIFVDYLRNGFGATAVAAFSP
                     RWRPGVGVSTPVAWDEIDEDIRGTHFNLLNVPARVAKLRKDPWAGYWKREQALTKAII
                     TKMGSGTDS"
     gene            complement(10564..11517)
                     /locus_tag="VEW08_14060"
     CDS             complement(10564..11517)
                     /locus_tag="VEW08_14060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HYJ41912.1"
                     /translation="MAKSKKNGNEDGEVEKPKRGARGLWNGAISFSLIHIPVSLHTAA
                     RAHELDLNLLDKRDFAPVGYQRYNKATGKVVEWNDVVKGYEYEKGEYVVLTDEDFRRA
                     NVEASRTIDIQTFVDRDAIAPYYFDTPYFLMPDKNGERVYSLLREALEQSKKLAVATF
                     VLRSRQHVAALMPVDKIIVLNTLRYSQEIQPHPDVAAAVAKKANAAGGRELTMALKLV
                     EEMSEKWKPEVFTDTYRDDLMKRIQQKVKAGQTHTLTEPEEEAAERPTGGAKVVDLMS
                     LLEKSLAQKGGSSGDAAARATKRKHSAARRRTASPRAARRA"
     gene            complement(11510..12601)
                     /locus_tag="VEW08_14065"
     CDS             complement(11510..12601)
                     /locus_tag="VEW08_14065"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYJ41913.1"
                     /translation="MRFAVVGLGHIAQAAVLPAFKHARPHVELTALVSDTPAKLKALG
                     RRYGVRQLYSYDDARDLFSSGAIDAVYIALPNTQHAAWTIRAADAGLHVLCEKPLATS
                     VHDCERMIEVCERNGVHLMTAYRLHFERCNLEVADIVRKKRIGEARYFDAQFSMQVKA
                     GNIRLKRELGGGPEWDIGIYCQNAARYVFADEPTQVWATATNSGDARFAEVPETVHVI
                     MKFPGERIANFVCSFGAADRSRYEIVGTKGSVVCDPAFEYAEGLSYELQVGEKKKRKK
                     FAKSDQFAPELVHFAKSVRSGKAPEPSGVEGLIDVAIIEAIHSSLATGGWAAVAAPQK
                     RRRPSLRQEIRRPAVPREPKLIQAESGHG"
BASE COUNT         2245 a         3930 c         4209 g         2426 t
ORIGIN      
        1 ccgaattacc cgaacggctc cgtggatacc cgcatgctgg cccaggcgtc gaaggcgctg
       61 atggtctaca acgagggccg cttgtacgac gagggagagc cggtgtacgt cgtcggcagg
      121 tgcaagccgt acgcgtcgtc gatgatttcg ccgtattcga agtactgaat cccgggggat
      181 cagggccggg cgaaaacctc ggccctggtc gaagcccaga ggatcagcag cgaggcgccg
      241 agcaccggta gcaaggcgcc ggcgcagacg atgagtgcga tcagcggcgc atggatcctg
      301 aatccctcga cgcgcgaggc gggtataccg agtgaaccct cgggccggcg ccgccaccac
      361 atcaccacgg ccgagacgca catcgtgatc aggccaagcg cggtgaacaa gccgagcagc
      421 tgattggcca gcccgaagag ctggccctca tgcgcggcga tgccgacgcc gatcacgcgg
      481 tctatggccg ctttctcgcc gaagccgtga tcccgcagca cttcgccgct tcgcgcatcc
      541 agctcgagct cgcgcacccg cgggcggttc tgcgtttcgg cgcgcacgcg ccagaatggg
      601 gtgtgctcgt tcggtagata gacgcgcacc gggggatcga gattgagcgg cgacactcgt
      661 ttggcgatgt cgtcgagcga gacgggcgtc gtgatcgact ccgccgcggc ttcagcgcga
      721 tgctgggtgt gttcgtcgac cgtggaaagt gcccactcct gcttttcggg cgtcagcagc
      781 gcgcgggttt tcgaaaggcc ggcgccccac accgtggtcc acggtaacgc ggtgatcagc
      841 aggaacatgg cgaaggcaga tacccagatg ccggtcacgg cgtgcaggtc gcgccagaac
      901 acgcgtcggc ccgcgctcgc gcgcggatac aagacgccgg cgatgccgcg gacatcgcgt
      961 ggccaccaga gatagaggcc ggtgcccagc atgacgatgg cccagctggc cgcgagctcg
     1021 atgaggatgg cgcctgggcg tccggccagc agttcggcgt ggatcatccg gacgatctcg
     1081 gtgagccggt gggtgtgcgg tacggacttg agtacggcca gcgtttccgg gtgcacatag
     1141 acaatgagtc cggtgccctc gggtgaatag aggtgcacgc gcaccgcatc atccggttcg
     1201 cgcgggatct cgtaaacgaa cagcttcgaa ccgggcaccg aggcgatggc ggcggcaatg
     1261 tgcgcctcac ccggcgcgcg cgcgccggtc actttcaggg agtccacgcc gcggtcggcg
     1321 aaggcgtcga tctgcggctt gaagaggtaa atggagccgg tcagggccag caccaccacg
     1381 aacgggatgc agaacatccc ggcgtggaaa tgccagcgcc acagggcgcg gtaggtgagg
     1441 ttcatggtgc cgggtgggga gtctggggag tgggaatgtt cacgtttacg acgcgttcac
     1501 agattctcat aaggtgacca cccgccgaca ctgcatccct cagtgttggc gaagaggtgg
     1561 gtgtgcctcg cagattgcga attcatgttc ccggcggctt ttatcacgtc acgcttcgtg
     1621 gaaatcacca gcaggatatt ttccgctcgg atggcgaccg ccaacttctc aatgccatcg
     1681 tggcgcgggc catcgaggcg tacggtgccc gcattcatgc ctactgctgg atgtcgaatc
     1741 acctgcattt cttgctgcaa gtcggtgatc agccactcgg gaacgtggtg cggcagatcg
     1801 cttcaggcta cgcgcgcgcc tttcagcaaa agctgcccac gaccggtcac ctgttcgagc
     1861 gccgacatca cgcttcgctc gtcgatgtgg actcgtatct cctggaactg ctgcggtaca
     1921 tccaccttaa tcccgtgcgg gcgaatctcg tttcgagacc ggcggagtat cgctggtcga
     1981 gtcacctggc ttacgcggga cgaaaggccg ctgaaccgtg gctaaccacc agttttgcgc
     2041 tcgccatgtt cagtgaggat gttcgcagtg cacacgcggc ttatggccgc ttcatggagt
     2101 gcccctcgac attcgggctc gaagagtcgt tgccttcaat caaggcaatt tccaccgaac
     2161 ctcgtcccct cgatcaagtt ccatcggcgt cgatcgcggt gatcccgcgg cagacgttgg
     2221 cacagatcat tgatgaaggc tgccggcgct ttggggttct cgagcaggac cttcccaccg
     2281 ccagccggga cccgctcctg gtgcaagcgc gggcctggat tgcgcgggag gcgttggaac
     2341 gccgggtttc gaccctgtcc gccgtggccc gcgcactggg acgagaccgc gccacgttgc
     2401 gcttcggcat tcgccttcgc cggtcttcag ggtaattcgg ggacattccg cgcaaccggg
     2461 gccccagaaa cgccactccc cactttcccc acccggcacc ctgaagtatc cttcgcggcc
     2521 tcaatcgaga ccctcggcag tcgcgtagct cagtggtaga gcacctcctt gacatggagg
     2581 tggtcggtgg ttcgatccca ctcgcgacta ccaaatcact tacccggtgc gcccatgtgg
     2641 ccctacggcg attttccgct gttggctgca cgctcttcga atcattatcg gagagtcgca
     2701 tgctcaagtt cctaggcagc accgttggca tcatctttct catcggtctc gttgtggtca
     2761 tcctgttgct caagttcatc ttctgaggtg ccggcgggtg tgcgcgcaat cccgctgaca
     2821 atcgtcctgc tgttcggctc agctgtcgcg gccgcggcgg gcgtcaagat cgagatcaac
     2881 ggcctgcccg aagagcagcg cgaggccgtg cgcgcgtccc tcgagctcaa caactacgac
     2941 aagcgcgaca tctcgcctgc cgaattgcgc gccgcatacc gcaaggccga cgaacagatc
     3001 aagagtgcgc tcgaaccttt cggtttctac gaccccagga tcactccaca tctgaccggc
     3061 gatgaagcca acggatggac cgcgaggttc gacgtggtgc ccggcgatcc ggcaatcgtc
     3121 cgcgtggcga aagtggaggt agcgggcgag ggcaaggaac agcggcgcgt cgccgaggcg
     3181 gtgagcgcgt ttgtgcccaa ggtcggtcag cggttcgatc acgccacgta cgaagccagc
     3241 aaggccgtgg tcgacacgag cctgcgcggc gcaggttttc tcgacgcgaa gtacctgacc
     3301 cgccgggtga ccgtgcggcc ggaggaacaa tccgccgaca tcgatctcac ctgggagagc
     3361 ggcattcgct acaggttcgg cgccgtgcgc ttttcgggtg atgcgccgtt ccccgaggaa
     3421 ttcctgcgtg agttcgtgtc atgggacgaa ggcgcctact tcaactccga acaggtgctc
     3481 aacctgcagc agcggctggt ggatgccgat tacttcgagc tggtgtcggt gcagccggcg
     3541 ctcgacgaga agaaggacgg cacggtgccg ctcgacatac tgctgaaacg cgacgaacgc
     3601 acggtgtact ccggtgaggt ctactacagc accgacttcg gcggtggcgt gcggttgggc
     3661 gcggatcgcc gctggctcaa caggaagggg cacaaggccg gcgcgaaggt cgaatactcg
     3721 gagcgcctgc aggaagcgga ggtgcactac aagattccgc ttccggggcg tgaagacagg
     3781 acgtacaact tcggcgtggg ctatcgcgac gagaccaccg acgtcaatcg ctcacagaat
     3841 ttccagattg cggccacgcg ttcggagaag cgctggcatg gattcacgcg cacgctggga
     3901 ttgaaatacc tggtcggcga cttcgagatc ggccaggacg aagacaatct ggagtacgga
     3961 aactccaatc tcctgttcgc cgaaggcacg ctgagccggc gccgcatcaa cgacaggctg
     4021 gcgccgcgga aaggctacgt gctcgagttc ggtggccggc tcgcggccga agcgctcgtc
     4081 tcggatacgg atctggcaca ggttttcggc aggatcacgt ggctgattcc gcagggtgag
     4141 cgcggtcgct tcaagttgcg cggcgaggcg ggcgccatga gcgtcggcaa cttcgatgcg
     4201 ctgccgcccg acctgcggtt ttacgccggc ggtgaccgct cgcttcgcgg tttcgactat
     4261 cacgatatcg gcgaggtcag cgcgaacggc aatatcattg gcggcgaatt tctcgccatc
     4321 gccagcgccg aatacgagta ctacttcacc gaggactggg gtgcgggggt gttcgtcgac
     4381 gcgggtgatg cattcaccaa cggcttcagt ctgaacgtcg gtgctggcct cggcattcgc
     4441 tggcgttcgc cgctggggcc gattcgtgtc gacgtggggt tccccgtgca atcggacctt
     4501 cccaccgcca cttcgtggcg gctgcacgtg ttgctggggc cggacctgtg aaggcgccgc
     4561 cgttcaagaa aattcttctg tggagtggcg gctcgctcgc gctgcttctg ctgctggtgg
     4621 cggcgttgct ggcctggttg ttgttcacga caccgggcgc tcgctgggtg gcgagtacgg
     4681 tcactttgcg gttcgcaccg caggtgaaat acgcgcgcat cgacggaacg atcgcgggcg
     4741 agctcaccgt gacggacctg cagttcgatg gcggcgccga caaggcgcga ataagaatcc
     4801 agtccataac cgtcgatccg acgttgatga tgttgttctc gcgtgcgctg cgcatcgaca
     4861 acgcgcgcgt gtccggactg accctgatcc tgccacccga gaaggatgag cccgagcccg
     4921 aagagccgct gtggatcgaa ccgccgctcg acctgacggt caacgacttc ctgctgaccg
     4981 atgcgactat ctaccgggag aacgagaaac tcgcgacgat caggcgcgcg gaactcagtg
     5041 cacgatggaa gtcgcgcgag ctgatcatcg agacgctgac agtgaaaccc ggcgacatcg
     5101 aaggcgactt cgtggtgtcg gggcgtattg cccccgaggg ggacaccgtg cgccttgcgt
     5161 tgaaggcgca gtggaaagaa gtcgtggttc cggagaatct ggccggccgc gtactcgcca
     5221 gtcacggcgg catcgacatc gagggcacgc cgaaggccta tgccgtgaaa ggcgcgctcg
     5281 atatcgggcc gcccgacgag ctcgttcatg tgctgatcga tgcatccggg actgacaagc
     5341 gcgcggatct gcgtcagctc gagctgcgcc agagcgcagg acatctggcg ttgagcggga
     5401 cggtcgaatt caagcccgtc gcgtggtcgt tgtacgccaa ggcgagcgac ttcaatcccg
     5461 gcgcgttgtt tgccgggtgg ccagggcgct tgaccatcga tgccagcacg cgaggtttgc
     5521 tcgcggaggc tgggccgcgc ggcgatctgc agattgcgac gctttcgggc acgttgcgcg
     5581 gacggccgat cgcgggcgag ggcgaaatcg agttcgaagc cccgtcgaaa tttgcaggtg
     5641 acctgcgcgt gtcctcgggc aagagccgcg tcaccgtgaa gggatcgagc ggcgatcgcc
     5701 agcagatcga tgcgaccgtg gatctggccg tggcctcgct caacgactgg gttccggaca
     5761 cgcgaggtag tttggcgggc cgcttcacca ttcgcggaga atggccaaaa ctcacgattg
     5821 cgggcagcgc cgacggcaag tctctcggca tgggtgagaa cgagatcgcg aagctgcacg
     5881 tcgatgcgac ggtggcgagc ccgctggatc cggatggcga attgcaggcc gtcgcgacac
     5941 agataaccct cggcggccgg atgcttgcca aggtgacgct ggatgcgacc ggcaaccagg
     6001 caaagcatcg cgtggcggtc agcgcggata gcgagcagta cgacgggacg gtggagctcg
     6061 cgggtggatt gacgaaaagg ggctggagcg gcgagctcac gagattgcag ctggaagcgg
     6121 tggatatcgg caccctcacg ctgcgcgaac cgtcacgcgt cgtctacgac gccggcaact
     6181 tctcactttc ccagacctgc cttgaagacc tgcccacggt gttgtgctcg gccatgaagt
     6241 tcgaggcgtc aggcgcgctc gatgcaggtt attcgttcga gcgcgtgccg ctgaagctgg
     6301 ccaatgcgct tgcgccggac gcgttggccg gcgagttgca gggcgacgta cagggtcatg
     6361 gccgcgtacg ccgcgacgcc aatggacagt ggataggcga tctggccgtt acctcggaat
     6421 ccgcgagaat ggtgctggct caaggcgagg accagagcga gtcggctgcg gcggcaacca
     6481 agctggcgac cgaagggtcc ttgctgatct acgaaaacct cggcttgcag gcggattttg
     6541 cgggcatgaa ggccaatgcg aagctgtctg caaagctcgc tcacggtggc tacttgaacg
     6601 tgtcgatgac ggtttccgat ctgaatgcgc cggcgccgcg cctcgcgggg ctgatccagg
     6661 cgaccatgcc gacactcgcg ccgtttggtg tattcgtgcc cgccgtggcg aacctcgatg
     6721 gcgcggtgaa tgctgaaatc gaagtgggtg gaacgacagc caagccggag ttcaccggca
     6781 acgtcgatgc cacgaagttg caggcggatc tcggtgcgct cggcatcaag ttgcgcgacg
     6841 gccggttgct tggtgaagcg cgccgcgacg gcggattcaa gctcgcgggt agcgtggcgt
     6901 ccggcaaggg ccacatcgag ctcgacggcg cgatggacga acgtggcgtg atcgaggcga
     6961 agatcatcgg ccagaacttc ctcgcggcgg atattcccgc ggcaaatgtc gtcgtgacgc
     7021 ccgacctcac gctcactgga gacccgaagg gctatctatt gcgtggcgag gtgaacattc
     7081 cgagcgccgt aatcaacctg cagaagtttc cgcaggacca ggcgcccggc gtgtcacccg
     7141 acgtggtggt gatccgcaat ggcaaggaag tggtggacga ggagagggcg aagtcgctgc
     7201 cgctcacggc ggtggtgacg gtgaatctcg gcgatgacat caaggtgacc ggatacgggc
     7261 tcgatgcgac ggtcaccggg caactgacgg tgcgtgaggc gccggacacg ccaacaaccg
     7321 gttccggcca ggtctcagtg gccgggcggt atgaggcgta cggtcaaaaa ctcaacatcg
     7381 aggacgggcg gctgttgttc gccggcacac cgctcgacaa tccacggctc gccatcaccg
     7441 cgatgcgcaa ggtcgacgag aacctctcga cgggcgtgcg catcgccgga acggcgaagc
     7501 gaccgatcat cacagtggtg tcgagcccgg aagtgggcga ggcggacgcg ttgtcgtacc
     7561 tggtgacggg ccgctcactg tcggaggttg gcagcgccag cggtagttcg caggatgcgc
     7621 tcaactccgc gcggcaatcg ttgtcgggtg ccggagcggg gctcgtggcc aagcgcatcg
     7681 gcgcgcggct cggcctcgat gaggcgggtg tcgaggagaa cgacttgatc ggcggctcgg
     7741 cgctcaccat cggcgaatat ctctcgccgc ggttgtacct gagttatggc gttgggctgt
     7801 tcgaacccgg cgaggtcatc gcactgcgct acaagctgac tgaagacgtg ggtgtgcgca
     7861 tccagcgcgg cactgaagag acgcgcaccg gcgtggaata ccgcatcgag aaataacggt
     7921 gccaggcacc gtttcctaga taatggtgcc aggcaccgtt tcctaggaat cggtgcctga
     7981 ccccattttt gtgatgatgg ccttggtcag ggcctgctcg cgcttccagt aacccgccca
     8041 cgggtccttg cgaagctttg ccactcgcgc gggtacgttg agcagattga agtgcgtgcc
     8101 gcggatgtcc tcgtcgatct cgtcccacgc gaccggagtg gagacgccga cgccgggccg
     8161 ccatcgcggt gagaacgcgg cgaccgccgt ggcgccgaag ccgttgcgca gatagtcgac
     8221 gaagatcttg ttcttgcgat tacgttcgcc ggtcttggac gtgaacatgg tcggcgcgag
     8281 cttctccatg aaacgggtga caccgagagc gaaccccttt gcggtgtccc aatccggacg
     8341 cggtgccccg agcggcacga cgacgtgcag tcctttgccg cccgtggtct tcacccagct
     8401 ttccagcccc aggtccttca tgagttttcg gatcgccagg gccgcatcgc gcaaaggtgg
     8461 atagggcaga tcggcgccgg gatccaggtc gaacgtgagg cggtcggcga gcctgggcct
     8521 gggcatgacc gcgccccagc tgtgaagctc gacggctcct acctgcgcca gatgcagcaa
     8581 gccctcgggc gattcgcaca cggtgaagtc cggatccttg tcggcgttcg gaatcttcac
     8641 cacgcgaagg ccgtcgcccg atttctcgag gaagtgcttc tggaagaatg gcttcgatcc
     8701 gtgcgtattg cgcagaatcg acagtggccg gtccttgtaa tgcggcatcg cctgctcacc
     8761 gattgccgcg aaatattcgg caagctgtag tttggtgatg ccatcgtgcg ggaacagcac
     8821 gcgatccggg tgggtgaggc ggccgagcga agcggggacc ttcgttttcg cagcggccct
     8881 gctggtgctc ttgccttggc ccttggcctt ggcgcgtttc gtttccttca cgacatccac
     8941 ggtgtgttcc ggctcctctc gaaccacggt ctttggatcg cgatcctgtc gcatgccgag
     9001 atatctcggt tgccgcagta tgcctccgtg cgtccagtcg gcgaacgcca cctggatcac
     9061 gagttcgggc ttcatccaat gccagatctc gcctgaacgc ttctcggggt ggttgtcgaa
     9121 cggactgctc ttctgggcaa gctttttcga ccgctcatgc cactcggcac gttcgttcgc
     9181 atcgaaacca ccgccgaccc ggccgacata tttgagcccc ttgccggatt tcacgccgac
     9241 cagcagcgat gaaaatgtcc ccgtgccgcg gccgggtaga aaggcggcgc ccccgaccac
     9301 gaactcctgt tcacggtggc atttgacctt gagccagttc gccgagcgat cgccgatgta
     9361 cggcgcatcg atcttcttcg ccacgaggcc ctcgagatgc tggttgcaca cgcgcgtgag
     9421 catctcggcg ctgtcgctat gaatctgatc ggcaagtcgc agcgggccct ttgccggcag
     9481 cttcgcgaac gccgcgtcga gccgcgtctt gcgttcggac aacggcaatc cgcgcaggtc
     9541 ttcacctcgg taatagagca ggtcgaagat gcaaagctgc agctcatcga ggcgaccctg
     9601 ctccatcgag tgttgcaaca gcgaaaaatc agaacggccg ttggggtctg tgacgatcag
     9661 ctcgccgtcc acccaggcgc catccagctt catcttcgcc agtgcctgga ccaccgggtc
     9721 cactttgcgc gtccagtcga ttccattgcg cgagatacag cgcacgtcgt cgccgtcgca
     9781 acggctgagc aggcgaacgc cgtcgtattt gagctcgtac acccaacctt cgtcgcccgg
     9841 taccttgtcg accagcgtgg caagcatggg cttgaggctc gcgggcagtg cgtggccgcg
     9901 ggcgcctttc gatctggatg aacgaacctt ggggacgccg ccaggtttcg gtttgggtac
     9961 gaacgccggc ctcgcggccg tgcgacgttt gagcatccgc ttcggatcgt cggctttccc
    10021 ggcgtcgatg atgtcggcat cggcgcccac gcgcgccgcg aagtcaccgc tcttcatgag
    10081 cagccagttc ggctgcttgc cctgcatgcg cgtgcgcacc aggtgccagc ggcctgacag
    10141 tttctttccg aacaactcga attcgaggtg gcctttcttc aggcccttgg cgaaatcgcc
    10201 gacgggctcc cacgtgccgt tgtcccagat gtcgacgtgg ccgccgccgt attgcccctt
    10261 gggaatgtca ccctcgaact tcgcgtacgc gagagggtga tcttccacct cgaccgcgag
    10321 tcgcttgtcg gccggatcga gagaagggcc tttgggcacg gcccagcttc gcagcgtgcc
    10381 tgcccactcc agccgaaagt cccagtgcag ccgccgcgcg gcgtgcttct ggaccacgaa
    10441 gatgaccttc ttgttgcgag cgcctggcgc cttgtgggaa ggctcggcag tcttcctgaa
    10501 gtcgcgcttc gcgacgtacc gtttgaggcg agtcggactg cgatttccgg cgtcggccat
    10561 gcatcaggcg cgacgtgcgg cgcgcggact ggccgtgcgg cgtcgcgccg cactgtgttt
    10621 acgcttggtg gcacgcgctg ccgcgtcacc cgaactaccg cctttctgtg cgaggctctt
    10681 ttccagcagc gacatgaggt ccacgacctt ggcgccaccg gtgggacgct cggccgcttc
    10741 ttcctcgggc tcggtgagtg tgtgtgtctg gccggccttc accttctgct ggatacgctt
    10801 catgagatcg tcgcgatagg tgtcggtgaa aacctcgggc ttccatttct cgctcatctc
    10861 ttcgacgagc ttcagcgcca tcgtgagttc gcggccgccc gcggcattcg ccttttttgc
    10921 gaccgccgcg gccacatccg ggtgtggctg gatctcctgg ctgtagcgca gcgtgttcag
    10981 cacgatgatc ttgtcgaccg gcatcaacgc cgcgacgtgc tgtcggctgc gcaacacgaa
    11041 cgtcgccacg gcaagcttct tcgattgctc cagcgcttcg cgcagcagtg aatagactcg
    11101 ctcgccgttt ttgtcgggca tgaggaagta gggcgtgtcg aagtagtaag gcgcgatcgc
    11161 gtcgcgatcg acgaaggtct ggatgtcgat ggtccgagac gcttcgacat tggcgcgtcg
    11221 aaaatcctcg tccgtaagca ccacgtattc gcccttttca tattcatacc cctttacgac
    11281 gtcgttccac tcgaccacct tgccggtggc cttgttgtat cgctggtagc cgacgggcgc
    11341 gaagtcgcgc ttgtcgagca ggttgaggtc gagttcgtgc gctcgcgccg cggtgtgcag
    11401 gctcacggga atgtggatca ggctgaagct gatcgcgccg ttccagaggc cgcgcgcacc
    11461 tcgctttggt ttctcgactt cgccatcttc attgccattc ttcttcgact tagccatgac
    11521 cgctctccgc ctggatcaac ttgggttcgc gcggcacggc cgggcgacga atttcctgtc
    11581 gaagcgatgg ccgccggcgc ttctgaggcg ctgcaacggc ggcccaacca cccgtggcga
    11641 gggagctatg gatggcttcg atgatcgcga cgtcgatcag gccttcgaca cccgacggtt
    11701 ccggcgcctt gcctgaccgg acagatttgg cgaagtgcac gagttccggg gcgaactgat
    11761 cgctcttggc gaatttcttg cgcttcttct tctcgccgac ctgaagctcg tagctcaggc
    11821 cctcggcgta ctcgaaggcc ggatcgcaga cgacgctgcc cttggtgcca acgatctcgt
    11881 accgcgagcg gtcggcggcg ccgaagctgc agacaaagtt ggcgatgcgt tcgccgggaa
    11941 atttcatgat gacgtggacc gtctcgggga cttccgcaaa tcgcgcatcg ccgctgttcg
    12001 tggccgtggc ccatacctgc gtgggctcat cggcgaacac gtagcgcgcc gcgttctggc
    12061 aatagatgcc gatgtcccac tcgggcccgc cgccgagttc gcgtttgagg cggatgttcc
    12121 cggctttcac ctgcatggag aactgggcgt cgaagtagcg cgcctcaccg atgcgcttct
    12181 tgcgaacgat gtccgccact tcgaggttgc agcgctcgaa gtgcagccgg taggcggtca
    12241 tgaggtgcac gccattgcgt tcgcagacct cgatcatgcg ttcgcagtcg tgcacgctcg
    12301 tggccagagg tttctcgcac agcacatgga ggccggcatc cgcagcgcgg atcgtccacg
    12361 cggcgtgctg agtgttggga agggcgatgt acaccgcatc gatggcgcca ctcgagaaca
    12421 ggtcgcgggc gtcgtcgtac gagtacagct gcctgacgcc ataacggcgg ccgagcgcct
    12481 tcagcttggc cggcgtgtct gaaaccaacg cggtgagttc gacatgcggg cgggcgtgtt
    12541 tgaacgccgg cagcacggcg gcctgggcaa tgtggccgag accgacgacc gcaaatcgca
    12601 cgggacgcgc tgatctggaa ggtgatttca cgacttcgag tagaaagccc gcgcgcccga
    12661 gccgatatca gccaagctcc cgcgccactg tcggaattga ttctgccggg ccgcactggc
    12721 aggatgctgc gccttccaac tgcttgatct gattccgaaa tccctatgcc tacgattacg
    12781 ttgccggacg gcagccaaaa gaatttcgaa
//
LOCUS       DASQJS010000357         4570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3991741, whole genome shotgun sequence.
ACCESSION   DASQJS010000357 DASQJS010000000
VERSION     DASQJS010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3991741"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /gene="rimI"
                     /locus_tag="VEW08_14070"
     CDS             <1..478
                     /gene="rimI"
                     /locus_tag="VEW08_14070"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HYJ41914.1"
                     /translation="RREDVREVPEVHIRPMMEIDLPDVAAIEQKSYAFPWSENIFRDC
                     LRVGYTCRALDLSGQIIGYGVMSLGAGEAHVLNVCVRDEYRSLGFGRRLLEHLLERAA
                     ASGVHEAFLEVRPSNLAAIRLYQRLGFEQIGIRRGYYQAPDGREDAIVLKLVLRQA"
     gene            498..2081
                     /locus_tag="VEW08_14075"
     CDS             498..2081
                     /locus_tag="VEW08_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HYJ41915.1"
                     /translation="MTSTGHETARRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGTV
                     KGRKAARHATSDWMALEKQRGISITSSVMQFPFRGEVINLLDTPGHEDFSEDTYRTLT
                     AVDSALMVIDCAKGVEERTIKLMEVCRMRDTPIMTFINKLDREGRPPIELLDEIESVL
                     KIRTAPVTWPIGMGRDLAGVYHLLEDRIYVYEAAARGERGTNRTIEGLHSEQGREFLG
                     DRAARFDDEIELVRGGTAEFNVDAYLDGRQSPVFFGSAISNFGVEELLAHFARHAPAP
                     LPRASVQREVASDEEDLTGFVFKIQANMDPQHRDRIAFMRLCSGKYTRGMRMYHVRLD
                     KEMRIADALTFMAADRSAADEAFAGDIIGLHNHGTINIGDTFTEGEKLTFTGIPNFAP
                     EMFRRAVLKDPLKMKALAKGLAQLCEEGATQLFKPLRNNDMILGAVGQLQFEVVAFRL
                     QDEYAVASVFETIAVHTARWVYCEDEKHLEEFRTKAYDHLAVDHSGALVYLAPSRVNL
                     ELTIERWPKIAFRSTRESQ"
     gene            complement(2026..2919)
                     /gene="rhlP"
                     /locus_tag="VEW08_14080"
     CDS             complement(2026..2919)
                     /gene="rhlP"
                     /locus_tag="VEW08_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04179.1"
                     /note="RhlP (RHombo-target LipoProtein) is a family of
                     predicted lipoproteins that, in general, co-occurs with a
                     form of rhombosortase, and that has an apparent cleavage
                     site for that enzyme, a GlyGly motif, near the C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhombotarget lipoprotein"
                     /protein_id="HYJ41916.1"
                     /translation="MTRTLRRNLRKVRLLAVATLSLALTGCSWLFDDRHFGREQQAST
                     PLVDFLYANGKVPLQDAQPELQLPIRVGVSFLPGGSGMRNFQPGAVDRDKVLNALREH
                     FKSLPYVNEIVIIPNHYLHAGSDGLAQIEQLSRLYQFDLFALVSYDQIQDSLQNERSL
                     AYLTIVGAYFVRGDRNETHTLLDVAVIDPKSRALVMRAGGTSALKGNVTALDADRHSV
                     AQSSRGFEVATEQLIINFSRELTDFEQRVRDGTAPVKVVRRAPKSGGSGALDPLLLAA
                     LLALTATLAWNGRRSSATARS"
     gene            3016..3648
                     /locus_tag="VEW08_14085"
     CDS             3016..3648
                     /locus_tag="VEW08_14085"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYJ41917.1"
                     /translation="MGRRNEHSRDELKALALRAAGDIVTESGAAALSMREVARRIGYT
                     VGALYLVFTNLDDLIVHLNEQTIMELREALERIRGRTNQPAQNLRLLVAAYLGFALLH
                     TARWRLVFEHRLPEGQKSPPTYAGHTAAIFALVSDRLREAGVTRDAAATAELATALWS
                     GAHGICMLAVTGKLQVATQASVQRLLDLLLDKFLGTSMSTPSGRVARKPK"
     gene            3685..>4570
                     /locus_tag="VEW08_14090"
     CDS             3685..>4570
                     /locus_tag="VEW08_14090"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ41918.1"
                     /translation="MREFIKNRPVFAWALYDWANSAFATTVMAGFFPVFFQKFWSTGV
                     ASTVTTSRLGYANAAAGLVIALLAPVLGAIGDRGGRRKQFLMAWTLLGVGATFALYFV
                     AQGQWFAAAVLFVLATMGFNGGVVFNDSLLLDVAKPADFDRVSAFGYSLGYLGGGLLF
                     LIKVLMVSKPAWFGLADASQAVRASFVTVAVWWLVSTIPLMRVVQEQQRGPHLGFAAA
                     MRVGFRELGATIAHVRQYRTIVMFLLAYWFYIDGVNTIIKMAVNYGVSLGLDTNSLLT
                     ALLVTQFVGFPAALFFGYL"
BASE COUNT          806 a         1485 c         1432 g          847 t
ORIGIN      
        1 ccgccgcgaa gacgtgcgcg aggtgccgga ggtccacatc cggcccatga tggaaatcga
       61 cctgccggac gtcgccgcca tcgagcagaa gagttacgcg tttccgtgga gcgagaacat
      121 ctttcgcgac tgcctgcgcg tgggttacac ctgccgggcg ctcgacctct cgggccagat
      181 catcggctac ggggtcatga gcctcggcgc cggagaggcg cacgtactga acgtctgcgt
      241 gcgcgacgaa taccgcagcc tggggttcgg gcggcgcctg ctcgaacatc tgctggaacg
      301 cgcagccgcc tccggcgtgc acgaagcctt cctcgaagtc cggcccagca acctggccgc
      361 aatccgcctc tatcaacgtc tcggattcga acaaatcggc attcgtcgcg ggtattacca
      421 ggcccccgat gggcgcgaag acgccatcgt gctgaagctc gttcttcggc aagcctgact
      481 caccaaccgg gatttcaatg acttcgaccg ggcatgagac ggcgcgcagg cgcacgtttg
      541 ccatcatttc gcatcccgac gcgggcaaga ccacgctgac ggaaaagctg ctgctgttcg
      601 gaggcgcgat ccagctcgcc ggcaccgtca agggccgcaa ggccgcgcgc catgccacct
      661 cggactggat ggcgctggaa aagcagcgcg gtatctccat cacctcctcg gtgatgcagt
      721 tcccgttccg cggcgaggtc atcaacctgc tcgacacgcc cggccacgag gacttctccg
      781 aagacacgta tcgcacgctg accgccgtcg acagcgcgct catggtcatc gactgcgcca
      841 agggcgtcga agagcgaacc atcaagctca tggaagtctg tcgcatgcgc gacacgccca
      901 tcatgacttt catcaacaag ctcgatcgcg aaggccgccc gcccatcgag ctgctcgatg
      961 aaatcgagag cgtgctcaag atccgcaccg caccggttac ctggcccatc ggcatgggtc
     1021 gtgatctcgc cggcgtctac catctgctcg aagatcgcat ctacgtctac gaggcggcgg
     1081 cgcgcggcga gcgtggcacc aaccgcacga tcgaaggtct gcacagcgag caaggccgcg
     1141 agtttctcgg cgaccgcgcc gcgcgcttcg acgatgaaat cgagctggtg cgcggtggca
     1201 ccgcggaatt caacgtcgat gcctatctcg atggccggca gtcgccggtg ttcttcggtt
     1261 ccgccatctc caatttcggc gtcgaggagt tgctggctca cttcgcgcgc catgcgcccg
     1321 cacctctgcc gcgcgccagc gtgcagcgtg aagtggccag cgacgaggaa gatctcaccg
     1381 gcttcgtgtt caagatccag gccaacatgg atccgcagca tcgcgaccgc atcgcgttca
     1441 tgcgcctgtg ctcgggcaaa tacacgcgcg gcatgcgcat gtaccacgtg cggctggaca
     1501 aggagatgcg catcgccgac gcgctcacgt tcatggccgc ggaccgcagt gccgccgacg
     1561 aagccttcgc gggcgacatc atcggcctgc acaaccacgg cacgatcaac atcggcgaca
     1621 ccttcaccga aggcgagaag ctcacgttca cgggcatccc caatttcgcg ccggaaatgt
     1681 ttcgtcgtgc cgtgctcaag gaccctttga agatgaaggc gctcgccaag gggctggcgc
     1741 agctctgcga ggagggcgcc acgcagctgt tcaagccctt gcgcaacaac gacatgatcc
     1801 tgggcgccgt cggccagctg cagttcgaag tggtcgcgtt ccgcctgcag gatgaatacg
     1861 ccgtcgccag cgtgttcgag accatcgcgg tgcataccgc acgctgggtt tactgcgagg
     1921 acgagaagca cctcgaggag ttccggacga aggcttacga ccacctcgcc gtcgatcatt
     1981 cgggggcgct ggtgtacctc gcaccctcgc gcgtaaacct cgagctcacg atcgagcggt
     2041 ggccgaagat cgccttccgt tccacgcgag agtcgcagta agcgcgagca aagccgcgag
     2101 cagcagcgga tcgagtgcgc ccgatccgcc actcttcggt gcccgtctca cgaccttcac
     2161 aggcgccgtt ccatcgcgca cgcgctgttc gaagtcggtg agctcgcgac tgaagttgat
     2221 gatgagttgt tcggtcgcca cttcgaatcc gcgagaactc tgcgccaccg agtgacggtc
     2281 ggcatccagc gcagtcacgt tgcccttgag cgcgctggtt ccgccggcgc gcatcaccag
     2341 ggcgcggctc tttggatcga tgaccgcgac gtcgagcaaa gtgtgagttt cattgcgatc
     2401 accgcgcacg aagtacgcgc ccacgatggt cagatacgcg agcgatcgct cgttctgcaa
     2461 tgaatcctgg atctggtcgt aggacacgag cgcgaacagg tcgaactggt agagccgtga
     2521 aagctgctct atctgggcca gcccatcgct gccggcgtgc agatagtggt tgggaatgat
     2581 gacgatctcg ttgacgtagg gcagggactt gaagtgttcg cgcagcgcgt tgagcacctt
     2641 gtcgcggtcc acggcgcccg gctgaaagtt gcgcatgccg gatccgcccg gcaggaagct
     2701 gacgccaacg cgtatcggca gctgcagttc cggctgcgcg tcctgcagcg gaacctttcc
     2761 gttggcgtac aggaagtcca cgagcggcgt ggacgcttgc tgttcgcggc cgaagtggcg
     2821 gtcgtcgaac aaccaggagc agccggtcag cgcaagcgac agggtcgcca cggccagcag
     2881 tcttactttc cgcagattcc tgcgcagcgt tcgggtcatt ggcggtctcc tcaattgaac
     2941 ggtgctcaat tattaccagc taattgaaca gcgctcaata acaacgtaga cttcgccgcc
     3001 aacaggtctt caaacatggg tcgacgcaac gaacacagcc gcgatgagct gaaggctctc
     3061 gctttgcgcg ccgccggcga catcgtcacc gaaagcggtg ccgccgcctt gtccatgcgc
     3121 gaggtggcgc gacgcatcgg ctacacggtc ggcgcgctgt acctcgtctt cacgaatctc
     3181 gacgatctca tcgtgcatct caacgagcag accatcatgg agctgcggga agcgctcgag
     3241 agaatccgtg gacgtactaa tcagccggcg cagaacctga gactgctggt cgccgcgtac
     3301 ctgggcttcg cgctgctgca caccgctcgc tggcgcctcg tgttcgaaca ccgtctgccc
     3361 gaagggcaga agtcgccacc gacttacgcc ggccataccg cggcgatatt cgcgctcgtg
     3421 agtgaccggc tgcgcgaggc gggcgtcacg cgcgatgcgg ccgccacggc cgaactagcc
     3481 accgcgttgt ggagcggcgc tcatggcatc tgcatgctcg cggtgaccgg caagctgcaa
     3541 gtcgccaccc aggcctcggt gcaacgcctg ctggacctgc tgctggacaa gttcctgggg
     3601 acgtccatgt cgacgccttc gggccgagtg gctcgcaagc cgaagtgacg tgacggcgtc
     3661 acgcgggccg cgtagccttc ctccgtgcgc gaattcatca agaaccggcc cgtattcgcc
     3721 tgggcgctgt acgactgggc gaactccgcg ttcgcgacca cggtcatggc cgggttcttc
     3781 ccggtgttct tccagaaatt ctggagcacc ggagtcgcct ccacggtcac cacgtctcgc
     3841 ctcggttatg ccaacgcggc cgctggactc gtgattgcgc tgctggcgcc ggtgctcggc
     3901 gcgatcggtg atcgcggcgg tcgccgcaag cagttcctga tggcctggac cttgttaggc
     3961 gtgggcgcca cgttcgcgct gtacttcgtg gcccagggcc agtggttcgc cgccgcagtc
     4021 ctgttcgtgc tcgcgacgat gggtttcaat ggcggcgtcg tgttcaacga ttcgctgctg
     4081 ctggatgtcg cgaagcccgc ggatttcgac cgcgtatccg ctttcggtta ttcgctcggc
     4141 tatctcggcg gcgggctgct gttcctcatc aaggtgctca tggtcagcaa gcccgcgtgg
     4201 ttcggcctcg cggatgcgtc gcaggccgtg cgcgcatcct tcgtcaccgt tgcggtgtgg
     4261 tggctggtgt ccacgattcc gctgatgcga gtcgtgcagg agcagcagcg cggtccacac
     4321 ctgggattcg cggcggcgat gcgcgtcggt ttccgcgaac tcggcgccac catcgcgcac
     4381 gtgcgccaat accgcaccat cgtgatgttc ctgctggcgt actggttcta catcgatggc
     4441 gtcaacacca tcatcaagat ggcggtgaac tatggtgtat cgctcggtct cgacaccaac
     4501 agcctgctca cggcgctgtt ggtcacgcag ttcgtggggt tcccggccgc gttgttcttc
     4561 ggttatctcg
//
LOCUS       DASQJS010000358         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2106212, whole genome shotgun sequence.
ACCESSION   DASQJS010000358 DASQJS010000000
VERSION     DASQJS010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2106212"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VEW08_14095"
     CDS             complement(<1..353)
                     /locus_tag="VEW08_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41919.1"
                     /translation="METLNRRSLLLGLAATAAPAWASPRQQVWKFKSLRKIGGHLAQV
                     YGAPQSIKSPIGPAVLFDGKDDVLFIDAHPLAGAKTFTAELVFRPDGGAFEQRVLHLE
                     SSESPPAAPGKGSTR"
     gene            457..1236
                     /locus_tag="VEW08_14100"
     CDS             457..1236
                     /locus_tag="VEW08_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41920.1"
                     /translation="MSATSPQIAQRLRRIQNALGVTADGMLGPETLTALEARLDLAAS
                     RKTISLTCSRKALELILQFEIGSRAQYERLYAHPVWPGGESGVTIGIGYDLGFTSKQQ
                     IEVDWDPFLEKPERAALAAVQGAKGAVAGQLARGLRHIKIPLAAAESVFYASTLPRFA
                     RITRDAFPGVEKLPLDAQGALLSLVFNRGASLTGERRKEMRAIRDELAGGSSLARIAE
                     HLEAMQRLWPDARGLRERRVCEAEFVRGSRRRYLAGERVDL"
     gene            complement(1273..>2627)
                     /locus_tag="VEW08_14105"
     CDS             complement(1273..>2627)
                     /locus_tag="VEW08_14105"
                     /inference="COORDINATES: protein motif:HMM:NF025697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HYJ41921.1"
                     /translation="GTTFGMDFNPTVDRVRVVNSAGQNFRMNPNNGAFVDGTAGGVLN
                     MDGGINGPTTGVQETAYTNNTANSTVTTQYTLDHTTDALCIQNPPNAGTQTSCQTLSS
                     PVDAVLGFDIAPGTNSAGVAVLKLTGQTNETLANVNLTTGTVASLGSIGSGGIAGLAV
                     HQPAAVRFVGLSADGTQLVRFSSNAMGTAVSVAITGITAGDTLAGIDYRPQTGQLYAL
                     GVSDVANAGTLYLLDPQTGAATIVGAASAVTFVDAAGMPVDLPAAASGYGFDFNPTVD
                     RIRVTTQTGLNFRINPNNGAPVDGNLNDTAAPPAGINTDASINGGATGVSGAAYTNSF
                     GQALTGGVTTQYVIDATTNSLFIQNPPNSGVVTAGVAIKIGDVPLDFTEVSGFDIPSD
                     VRTATSGMPVPSGIAYAALTVAGEQRMYSIDLVSGAATELGTPSMVLSGLATGQIALR
                     "
BASE COUNT          444 a          814 c          880 g          489 t
ORIGIN      
        1 attcgcgtgc tgcccttgcc cggcgctgcc ggcggggatt cactggattc caggtgcaga
       61 actcgttgct cgaatgctcc gccgtccggg cgaaacacca gctcggctgt aaatgttttc
      121 gcgcctgcga gcgggtgggc atcgatgaaa aggacatcgt ccttcccatc gaacagcact
      181 gccggcccga tggggctctt gatcgactgc ggtgcaccgt agacctgcgc caggtggccg
      241 ccgatcttgc gcagactctt gaatttccat acctgctggc gcggggaagc ccaggctggc
      301 gcggctgtag cagccaggcc aagtagcaaa gatcggcgat tcagtgtttc cacgatgaaa
      361 gcttacacgc gaagcacgga acaaagtcac gactttgatc gactagctat cggcccgcgt
      421 tgactgtatt gctcgccagg cctagcgtcg gtatcgatgt cagccaccag tccccagatt
      481 gcgcagcgac tgcgccggat ccagaacgca ctcggcgtga cggccgacgg catgctcggt
      541 cccgagacgc tcacggcgct ggaggcccgt ctcgatcttg ccgcgtcgcg gaagaccatc
      601 agtctcacgt gctcgcgcaa ggcgctcgag ctcattcttc agttcgagat cggctcgcgc
      661 gcgcagtatg agcggctgta cgcgcatccc gtctggccgg gtggcgaaag cggcgtcacc
      721 atcggcatcg gctacgacct gggattcacg tcgaagcagc agatcgaggt ggactgggat
      781 ccgtttctcg agaaacccga gcgtgcggcg ctcgcggccg tgcagggcgc caagggcgcg
      841 gtcgccgggc aactggcgcg cgggctgcgc cacatcaaga ttcctctcgc ggcggccgaa
      901 tcggtgttct acgccagcac gctgccgcga ttcgcgcgaa tcacgcgcga cgcatttccc
      961 ggcgtggaaa aactgccgct cgacgcgcag ggcgcgctgc tgtcgctggt gttcaaccgg
     1021 ggcgccagcc tcaccggcga acggcgcaag gaaatgcgcg cgattcgcga tgagctcgcc
     1081 ggtggctcgt cgctcgcgcg aatcgcggaa cacctggaag ccatgcagag gctgtggccc
     1141 gatgcccgcg gattgcgcga acggcgagtg tgtgaggccg agttcgtccg cggttcgaga
     1201 cgccggtatc tggccggcga gcgggtcgac ctctgacaag tcttttcagt tggacgcctt
     1261 ctccgacctg tttcaccgca gcgcgatctg tcccgtggcc aggccggaca ggaccatcga
     1321 gggtgtcccc agttcggtcg ctgcaccgct gacaagatcg atcgaataca tgcgctgttc
     1381 gccggccacg gtcagtgcgg cgtaggcgat tcccgacggc accggcatac cggaagtggc
     1441 cgtacgcaca tcggaaggaa tgtcgaagcc gctcacctcc gtgaagtcca gtggcacatc
     1501 accgatcttg atcgccacgc cggcagtgac cactccgctg ttaggcgggt tctggatgaa
     1561 gaggctgttg gtcgtggcat cgatcacgta ctgcgtggtg acgccgcccg tgagcgcctg
     1621 gccaaagctg ttcgtatacg ccgcgccgga aacacccgtg gcgccgccgt tgatcgacgc
     1681 atccgtgttg atgcccgctg gcggtgccgc ggtatcgttg aggttcccgt ccacgggggc
     1741 gccattgttt gggttgatgc ggaagttgag accggtttgc gtggtgacac ggatgcggtc
     1801 gacagtggga ttgaagtcga agccatatcc gctggccgcg gcgggcagat ccacgggcat
     1861 tccagcggca tcgacgaaag tcacggcgct cgcggctcca acaatcgtcg cagcgccggt
     1921 ttgcggatcg agcaggtaca aggtgccggc gttcgcgacg tcggatacac caagcgcgta
     1981 gagctggccg gtctgcgggc gatagtcgat accggccagc gtgtcgccag ccgtgatgcc
     2041 ggtgatggcg acgctgacgg ccgtgcccat agcgttgctc gagaaacgca cgagttgcgt
     2101 gccgtcggcg gagagcccga cgaatctcac ggctgcgggc tgatgcacag cgaggccggc
     2161 aattccgccg gagccgatgg aacccagtga cgccacggtg cccgtagtta gattcacatt
     2221 cgccagcgtc tcattcgtct ggccggtcag tttgagcacc gcgacgcctg cgctattggt
     2281 ccccggcgcg atgtcgaagc ccagcaccgc atccacgggc gagctcaacg tctggcatga
     2341 cgtctgcgtt ccggcattcg gcgggttctg gatgcagagc gcatcggtgg tgtggtccag
     2401 ggtgtactgc gtcgtcaccg tgctgttggc ggtgttgttg gtgtaggccg tttcctggac
     2461 gcccgtcgtg gggccgttga tgccgccgtc catgttcagc actccgccgg cggtgccatc
     2521 gacgaacgca ccgttgttcg ggttcatgcg gaagttctgg ccggcactgt tgaccacgcg
     2581 aacacgatcg acggtgggat tgaagtccat cccgaaggtg gtgccag
//
LOCUS       DASQJS010000359         4695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1163801, whole genome shotgun sequence.
ACCESSION   DASQJS010000359 DASQJS010000000
VERSION     DASQJS010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163801"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VEW08_14110"
     CDS             <1..954
                     /locus_tag="VEW08_14110"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYJ41922.1"
                     /translation="VIHYGMARELVLGLEVVLADGRVMDTLTGLRKDNTGYDIKSLFL
                     GAEGTLGIITAACIKVYPALRTRATAFIAARDPAAAVSLLGALRQASGDCVTSFELIP
                     RIGVELTTRHIPGVVDPLSTPSPWYVLAELSSSRADEPLDAILQKVLENALGNQWVTD
                     AAMAQNDRERDAFWRLRESIPEAQRKDGASLKHDISVPVSQVPRFIDVAGTWVAQHIP
                     QGVLVSYGHLGDGNLHFNINQRAGTQGFQLQAVEQKVKRAIHDFVKEFGGSFSAEHGI
                     GQLKVFELERYANPVELDLMKRIKHALDPNNIMNPGKVLRP"
     gene            complement(971..1522)
                     /locus_tag="VEW08_14115"
     CDS             complement(971..1522)
                     /locus_tag="VEW08_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41923.1"
                     /translation="MAIKNPPVVQARLHTVTLATDGPAVAEELQAKGYEPIRFASNYP
                     VSDSVEASLWSVPEPVAASAIHFKSPAAAEANLRVLEMALAASSRSADSAVEKAFFRN
                     VLGADVPSWPAAVERTKKVRVQAWTYLISDILAANKRLRESGIPVVYDPVKITTAYLG
                     DHKTMAIRAPDGTIIELVEAAAQ"
     gene            1624..2724
                     /locus_tag="VEW08_14120"
     CDS             1624..2724
                     /locus_tag="VEW08_14120"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYJ41924.1"
                     /translation="MADKIECVVVGAGVVGLAVARALALAGREVLLLEAETHPGTITS
                     ARNSGVIHAGLYYAPGSFKARFCVAGNRALYAYCQARGVDHQNSGKLIVANGDEEEQV
                     LLHLLERAHLNDVDDVRMISGIEAHKLEPEVRCTAALLCPTSGIVDQHPYMLALQGDM
                     ENAGGTLVCDCRVESLARSNGGFLVKTAGDSATEIEARFVVNSAGLDAVGLLGRIEGY
                     PQERIPKMHLGRGNYFTVAARSPFKHLIYPVPHAAGLGIHATLDLGKRVRFGPDVEWI
                     DKIDYSVNTARAPLFYDAIRRYWPGLADGALMPDYTGIRPKLHGPGEPQPDFRIESAA
                     DHGLPGLVNLLGIESPGLTSSLAIGDYAASFA"
     gene            2734..3669
                     /locus_tag="VEW08_14125"
     CDS             2734..3669
                     /locus_tag="VEW08_14125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ41925.1"
                     /translation="MRKKTLPGLLTALVSSLAVFACSKQEPPPPPVAATASERAEPAP
                     GAARIAIAPDGVHVQYRVYGAGEPALVFIHGWSCDSNYWREQLPALDRKYTLVTVDLA
                     GHGGTDGNRTEWSIARFGQDVATALSAVPNKQIILVGHSMGGPVAIEAARLLRGRVVG
                     IIGVDTFKSIGAPLPSKAQVDAAIKPFEADFIGQTRSLVADHLFVKGAHPQLAQKIAY
                     DMSLSPPRVAVPVMRALLEYDFTGPLKDISVPVVAINSDLGEPVNEARIRKVLPKFRT
                     VTLPGTGHFLMMEDPQRFNPALEAEVAAMSGSAGS"
     gene            3677..4225
                     /locus_tag="VEW08_14130"
     CDS             3677..4225
                     /locus_tag="VEW08_14130"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HYJ41926.1"
                     /translation="MDARTRGGGPDGGPHGHDNRGREQRGLRGLERLRSITMVFDQAF
                     AVPGTKWRFGLDALFGLVPGLGDIAGGIVAAYALSVARNLNAPPVIQLHMLSNIALDA
                     LIGMMPVLGDLFDFAFKAQTRNLALLDAYVATPHKTARRSRRGLLLIPIAIIVVFATL
                     TALGVWMLVILFHWLGSLFPGV"
     gene            complement(4199..>4695)
                     /locus_tag="VEW08_14135"
     CDS             complement(4199..>4695)
                     /locus_tag="VEW08_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41927.1"
                     /translation="GRRCLESDGPENRWLDYRVVIGDGLFWYRRRRLDIPGDDLTEEI
                     AGFPHVDLDEARLFSCGISWSARDKGPRKVIDTFDLHDRGGRGHFRTPDGRALTLELH
                     GNDWPLSEGRESLVLILTDGKTDSEPIASSWTSLGAARVGIDIGWLAIDCAPVVSDTG
                     EQRS"
BASE COUNT          804 a         1572 c         1495 g          824 t
ORIGIN      
        1 gtcatccact acggcatggc ccgcgagctg gtgctgggcc tcgaagtggt gctcgccgac
       61 ggccgcgtga tggacacgct caccgggctg cgcaaggaca acacaggcta cgacatcaag
      121 tcgctgttcc tgggcgccga aggcacgctc ggcatcatca cggccgcgtg tatcaaggtc
      181 tacccggcgc tgcgcacgcg cgccaccgca ttcatcgccg cgcgcgatcc ggcggccgcc
      241 gtgtcgttgc taggtgcgtt gcggcaggcc agcggcgact gcgtcacctc gttcgaactc
      301 attccgcgca tcggtgtcga gctcaccacg cgccacattc cgggcgtcgt ggatccgttg
      361 tccacgccat caccgtggta cgtgctcgcc gagctttctt cttcgcgagc cgacgagccg
      421 ctcgacgcga ttctccagaa ggtgctggaa aacgcgctcg gcaatcagtg ggtcaccgat
      481 gcggccatgg cgcagaacga ccgcgagcgg gacgctttct ggcggctgcg cgagtccatc
      541 cccgaggcac agcgcaagga cggcgccagc ctcaagcacg acatctccgt gccggtgtcg
      601 caggtgccgc gcttcatcga cgtcgcgggc acgtgggtgg cgcaacacat tccgcaaggt
      661 gtgctcgtgt cgtacgggca cctgggcgat ggcaacctgc acttcaacat caatcagcgc
      721 gccggcaccc agggcttcca gttgcaggcc gtggagcaga aggtgaagcg cgcgatccac
      781 gatttcgtga aggagttcgg tggcagcttc tccgccgaac acggcatcgg ccaactgaag
      841 gtcttcgagc tggagcgtta tgccaacccg gtggagctgg acctcatgaa gcgcatcaag
      901 cacgcgcttg accctaacaa catcatgaac ccgggaaaag tattgcgccc ttgagcgcga
      961 acaattgccg ttactgcgcg gcagcctcca ccagttcgat gatcgtgccg tcgggcgcgc
     1021 ggatggccat ggtcttgtga tcgccgaggt atgcggtggt gatcttgacc gggtcgtaca
     1081 ccacgggaat gccgctttcg cgcaggcgct tgttagccgc caggatgtcg gagatcaggt
     1141 aggtccaggc ctggacacgt acttttttcg tgcgctcgac tgccgcgggc cagctcggca
     1201 cgtcggcacc cagcacgttt ctgaagaacg ccttttccac agcggaatcc gccgaccggc
     1261 tgctcgcagc cagcgccatc tcgagcaccc ggaggttggc ttcggccgcc gcgggcgact
     1321 tgaagtggat ggcactcgcg gcgaccggct ccggcacgct ccacagcgac gcctcgacgg
     1381 aatccgacac gggatagtta gaggcgaacc gaattggctc gtagcccttc gcctgaagct
     1441 cttcggcgac cgcgggacca tcggtggcca gcgtgacggt gtgcagccgg gcctgcacga
     1501 ccggagggtt tttgatcgcc atgtaagggt tgccggagga cccgctgcag gaagccaggg
     1561 cagctagcaa aagggctccg aggacgcggc agacatggga tgtttgcatg ataaattgag
     1621 tatatggcag acaagatcga atgcgtcgtc gtcggtgcgg gcgtggtggg cctcgccgtc
     1681 gcgcgcgcat tggcactggc cgggcgcgaa gtgctgttac tcgaagcgga gacccatccg
     1741 ggcaccatca cctcggcgcg caacagcggc gtgattcatg cgggcctcta ctacgcgccg
     1801 ggcagtttca aggcgcgctt ctgcgttgcc ggcaatcgtg ccctgtacgc ctattgccag
     1861 gcgcgcggcg tcgatcacca gaacagcggc aagctcatcg tcgccaatgg cgatgaagaa
     1921 gagcaggtgc tcctgcacct tctcgagcgc gcccacctca acgatgtgga cgacgtgcgc
     1981 atgatctccg gcatcgaagc ccacaagctc gaacccgagg tgcgctgcac ggcagccctg
     2041 ctctgcccca cgagcggcat cgtcgatcag catccctaca tgctcgcgct gcagggcgat
     2101 atggaaaatg ccggcggcac cctggtgtgc gactgccgcg tcgaaagtct cgcccgttcg
     2161 aacggcggct tcctcgtgaa aacggcgggc gacagcgcca ccgaaatcga ggcgcgcttc
     2221 gtggtgaaca gcgcgggtct cgatgccgtc gggttgctgg gccgcatcga gggttatccg
     2281 caggaacgca tcccgaagat gcacctcggg cgcggaaact atttcacggt cgccgcgcgt
     2341 tcaccgttca agcacctcat ctacccggta ccacacgccg caggcctcgg cattcacgcc
     2401 acgctcgatc tcggcaagcg cgtccgattc ggccccgacg tggagtggat agacaagatt
     2461 gactactccg tgaacaccgc gcgcgcgccg ctgttctacg acgccatacg ccgctactgg
     2521 cctggccttg ccgacggcgc gctgatgccc gactacaccg gcatccggcc aaaacttcat
     2581 ggcccgggcg agccgcagcc ggactttcgt atcgagtcgg cggctgatca tggcctgccc
     2641 ggtctcgtga acctgctcgg catcgaaagc ccggggctca cctcgtcgct cgccatcggt
     2701 gactacgcag cctcgttcgc ctaaagtccg cgcatgcgaa aaaaaactct gcccgggttg
     2761 ctgaccgctc tcgtttcttc tttggccgtc tttgcctgca gcaagcagga acctccgcca
     2821 ccaccggtcg ccgccaccgc gtctgagcgc gcggagccgg ctcccggcgc ggcacgcatc
     2881 gcgatcgcgc ccgacggcgt gcacgttcaa taccgcgtgt acggcgccgg tgagcccgcc
     2941 ctggtattca ttcacggctg gtcctgcgat tcgaactact ggcgcgaaca gctgcctgcg
     3001 ttagacagga aatacacgct ggtcaccgtg gatctggcgg gccatggcgg caccgacgga
     3061 aaccgtacgg agtggagcat cgcccgcttc ggccaggacg tggccaccgc gctgtcggcg
     3121 gtacctaaca agcagatcat cctggtcggt cactcgatgg gcggaccggt ggccatcgaa
     3181 gccgcgcgcc tcctcagggg ccgcgtcgtc ggcatcatcg gcgtcgatac cttcaagtcg
     3241 atcggcgcac cattgcccag caaggcgcag gtggatgccg cgatcaagcc gttcgaagct
     3301 gacttcatcg ggcaaacgcg ttcgctggtc gccgatcatc tgttcgtcaa gggcgcccac
     3361 ccgcagctgg cgcagaagat cgcctacgac atgtcgctgt cgccgccgcg cgtcgcggtg
     3421 ccggtgatgc gcgcgctgct cgagtacgac ttcaccgggc cgctgaaaga catttcggtc
     3481 ccggtcgtcg ccatcaactc ggatctcggc gagccggtca acgaggcacg aatccgcaag
     3541 gtgttgccga agttccgcac cgtcaccctg cccggaaccg gccacttcct catgatggaa
     3601 gacccgcagc gcttcaaccc ggcgctggaa gccgaagtcg cagcgatgtc cggatcggcg
     3661 ggtagctagt ctcgccatgg atgcgcgcac cagaggcggc gggcccgatg gcggccccca
     3721 cggccatgac aaccgcgggc gcgagcagcg cggcctgcgt gggctcgaac gcctgcggtc
     3781 gatcaccatg gtgttcgacc aggcgtttgc cgtgcccggc acgaagtggc gcttcggtct
     3841 cgatgcgttg ttcggcctgg tgccgggcct cggcgacatc gcgggcggga tcgtggcggc
     3901 gtatgcgctg agtgtggcgc gcaacctgaa cgcgcctccc gtgatccagc tgcacatgct
     3961 cagcaacatc gcgctcgatg cgctcatcgg catgatgccg gtcctggggg atctgttcga
     4021 cttcgcgttc aaggcgcaga cgagaaacct cgcgctgctc gacgcttacg tggccactcc
     4081 gcacaagacc gcgcgtcgca gccgtcgcgg cctgctgctg atacccatcg ccatcatcgt
     4141 cgtgtttgcc acgctcacgg cgctcggcgt gtggatgctc gtcatcctgt tccattggct
     4201 aggatcgctg ttccccggtg tctgatacga ccggcgcgca gtcgatagcc agccagccga
     4261 tgtcgatacc cacgcgggcc gcgccgagcg acgtccagct ggacgcgatg ggctcgctgt
     4321 cagtcttgcc gtccgtcagt atcagcacca gcgattcgcg tccttcggac agcggccagt
     4381 cgttcccgtg cagctcgagc gtgagtgcgc ggccatccgg cgtgcggaag tgtccgcgcc
     4441 cgccgcgatc gtgcaggtcg aaggtatcga tgacctttcg cggccccttg tccctggccg
     4501 accacgaaat gccgcacgag aagagccgcg cttcgtcgag atccacgtgc gggaatcccg
     4561 cgatctcttc ggtgagatcg tcgcccggaa tgtccagccg gcgcctgcga taccagaaca
     4621 atccgtcacc gatcaccacg cggtagtcga gccagcgatt ttccggtccg tcggattcca
     4681 ggcacctgcg tccgc
//
LOCUS       DASQJS010000360         4988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2110725, whole genome shotgun sequence.
ACCESSION   DASQJS010000360 DASQJS010000000
VERSION     DASQJS010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110725"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..437
                     /locus_tag="VEW08_14140"
     CDS             90..437
                     /locus_tag="VEW08_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019581848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HYJ41928.1"
                     /translation="MASIFLATEIKLRTRSRVLEVAFDDGARYLLPFEYLRVHSPSAE
                     VKGHGPGQEVLVLGKQNVGIKAVEPVGQYAVKLVFDDGHDSGLYSWKYLRELGDNQQV
                     NWLKYQARVQAAQ"
     gene            672..1430
                     /gene="ubiE"
                     /locus_tag="VEW08_14145"
     CDS             672..1430
                     /gene="ubiE"
                     /locus_tag="VEW08_14145"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008080626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HYJ41929.1"
                     /translation="MTEHEKNADTDFGYQRVPWNEKQRRVRGVFDSVAGNYDLMNDLM
                     SGGAHRLWKEFTLSLTSLRPGQRALDVAGGTGDLTTGLAKQVGKTGIVILSDINAAML
                     GEGRDRLLDSGVVGNVSCVLANAEKLPFADAMFDCVTIGFGLRNVTDKAAALADMRRV
                     LKPGGQLLILEFSQPVVPLLKPLYDLYSFRVLPLIGKLVARDEDSYRYLAESIRMHPD
                     QETLLGMLRDAGLEDCRYHNLSGGIVAVHRGYRY"
     gene            1468..1755
                     /locus_tag="VEW08_14150"
     CDS             1468..1755
                     /locus_tag="VEW08_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41930.1"
                     /translation="MIVIGIAFVPVFIAAVVILRSVSRAFLLAATFTAGAFAGFVGCG
                     LLGDWLMRSPTTGPGSTASLVAFATAGAIGGGMLAVYLLGRFSRYPPWRRH"
     gene            1771..2388
                     /locus_tag="VEW08_14155"
     CDS             1771..2388
                     /locus_tag="VEW08_14155"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HYJ41931.1"
                     /translation="MLTQAIENLLNRNLPRSPRAQALCAALSGKRVRVEARGLAWVLL
                     IESLNTSVKLTKDGDGGQPPDAEISGTLLNLAALAGSHPEEVIQRGDVSISGDAEVAQ
                     QFRELAMLLKPDVEEELSRIIGDTPAHQALRLVRMATGFGRRAATTGMRNVAEYLAHE
                     RRDLVPRAEAEDFYRGVERLREDLDRLEARTGQLGEAGVEDEVQR"
     gene            2385..4049
                     /gene="ubiB"
                     /locus_tag="VEW08_14160"
     CDS             2385..4049
                     /gene="ubiB"
                     /locus_tag="VEW08_14160"
                     /inference="COORDINATES: protein motif:HMM:NF003404.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HYJ41932.1"
                     /translation="MKLRVLSRLIEIQRVLVRHGLDEFVRATHLYRPLRFIFLLSPWT
                     WAVRRGDAPRAERLRLALEELGPIFVKFGQALSTRRDLLPLDIADELAKLQDRVPPFD
                     GKVARAVIEAAYGRAAEQVFSVFEEEPLAAASIAQVHTAELRAEVPLNGAKTREVVVK
                     VLRPGMHAIIARDLEVLRALAAFAEANWEGSQRLKPLEVVAEYEKTIVDELDLMREAG
                     NASQLRRNFENSPLLYVPAVHWDFCRPGVMVMERIRGVQISNMARLREAGTDIRKLAE
                     NGVEIFFTQVFRHNFFHADMHPGNIFVLIDNPAQPKYAAVDFGIVGTLDPRDQYYLAE
                     NFLAVFDRDYRKVATLHLESGWVPEGSRVDEMESAIRTVCEPIFNKPLKEISFGTVLL
                     RLFEISRRFNVSIQPQLILLQKTLLNIEGLGRDLYPDLDIFKTAGPIMREWVREKMSG
                     RALLENARTQLPEILTSLQSLAPLVRSAVQRAQDGRLRLAVEAPEINALRTEIRRTNR
                     RRDKVTIAAVLLLGGLVWMAVGRDPAWAGWALSGAGAAWLLGLLRR"
     gene            4132..>4988
                     /locus_tag="VEW08_14165"
     CDS             4132..>4988
                     /locus_tag="VEW08_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41933.1"
                     /translation="MVIPESAEPSRNLAQFVAFVALAVGLFVLAGWAFGMDQLTNIVP
                     TWPRVVRLTALAFILSGFALWLATIQKRVPAIIAAGLLTAIGAIVLLKYATGWDVYLD
                     QLSLAQMPQVLDGNTPPRMAPASALAFFLLGASLLFAQRPRGALIHQGLAVVGMVLGW
                     LGLSRYMFGGEPLFPFADMAVHTATLLLMLTGATLTLRPDAGVARLLASGGVGGAMAR
                     RLLPAAIIVPLVAGSLTLYFERHHTLSFATAVAFFALASVVVFVAFVWINAARGERAD
                     SLRRAAERAL"
BASE COUNT          844 a         1636 c         1577 g          931 t
ORIGIN      
        1 ccccccggtg caaaaacgcc ggttttcccc ctccggcgcg tggtcgagta taggcttgcc
       61 gcccacctat gacgatcttt ggctaaaaca tggcctccat ctttttggcc accgaaatca
      121 agctccggac gcgctcacgc gtgctggaag tcgctttcga cgatggcgcg cgctacctgt
      181 tgcctttcga atacctgcgc gtgcactcgc cctcggccga agtgaagggc cacggacccg
      241 ggcaggaggt cctggtgctg ggaaagcaga atgtcggtat caaggccgtc gagcccgtag
      301 gccaatacgc cgtcaagctg gtgttcgacg acggccacga ttcggggctg tattcgtgga
      361 agtacctgcg cgagctgggt gacaaccagc aagtgaactg gttgaagtac caagcccgag
      421 ttcaagctgc ccaataggct tcatgtagcc ggccgaaggc cggcgttgaa ttcgcgccgc
      481 aggcgctggc aatacggcgt tgaattcgcg gcgcagccgc tgacaatccc aataaataaa
      541 ggccggcgat cgtcaaaccg ccggcctttc ccaagacccc cgctcgaata gttgggcttt
      601 gtagcgaatg cacgaccgaa aagccagcgc tttcgagccg cgtttgcgac accgtctata
      661 ctggccgccc tgtgaccgaa catgaaaaga acgccgacac cgatttcggc taccaacgcg
      721 ttccctggaa cgaaaagcag cgccgtgttc gcggcgtgtt cgactcggtt gcaggcaact
      781 acgacctcat gaacgacctg atgtccggcg gcgcccatcg cctctggaag gagttcacgc
      841 tgtcgctgac cagcctgcgt cctggccagc gcgcgctcga tgtcgccggc ggcacgggcg
      901 acctgactac cgggcttgcg aaacaggtcg gcaagaccgg catcgtgatc ctgtcggaca
      961 tcaacgccgc catgctcggc gagggccgcg atcgcctgct cgactcgggc gtggtcggca
     1021 acgtgtcctg cgtgctcgcg aacgccgaaa agctgccgtt cgcggacgcc atgttcgact
     1081 gcgtcaccat cgggttcggc ctgcgcaacg tcacggacaa ggcggccgcg ctcgccgaca
     1141 tgcgccgcgt actcaagccc ggtggccagc tgctcatcct cgagttttca cagccggtgg
     1201 tgccgctgct caagcctctt tacgacctgt attcattccg cgtgctgccg ctgatcggca
     1261 aactggtcgc cagggacgag gacagctatc gctacctcgc cgaatccatc cgcatgcatc
     1321 ccgaccagga aaccttgtta ggcatgctgc gcgacgcggg cctcgaggat tgccggtacc
     1381 acaacctgtc tggtggcatc gtcgcggttc atcgcggata ccgctactag ctggctcatg
     1441 ggacccgcct tcgtcgcagc tgcatggatg atcgttatcg gcatcgcctt cgtgcccgtg
     1501 ttcattgccg cggtggtcat cctgcgttcc gtgtcacgcg cattcctcct ggctgcaaca
     1561 ttcacggcgg gggcattcgc ggggttcgtc ggctgcggct tgctcggtga ctggctcatg
     1621 cgctctccca ccaccgggcc gggttcaacc gcaagcctcg tcgctttcgc gacggccggc
     1681 gccatcggcg gcgggatgct cgccgtgtac ttgctcggac gattttccag gtatccgccc
     1741 tggcgacgtc attagcggtt tgagtccagg atgctgaccc aggcgatcga gaatctgctg
     1801 aaccgcaatc tgccgcgcag cccgcgcgcg caagcgctgt gcgccgcact gtcgggcaag
     1861 cgcgtgcgcg tcgaagcgcg cggcctggcc tgggtgctgc tcatcgaatc gctgaacacg
     1921 tccgtgaagc tcacgaaaga cggtgacggc ggacagccgc cggatgccga gatctccggc
     1981 accctgctga atctcgcggc gctggccggt tcgcatccgg aggaagtcat ccagcgcggc
     2041 gacgtgagca tcagcggcga cgcggaggtt gcccagcaat tccgcgagct cgccatgctg
     2101 ctgaagcccg acgtcgaaga ggaactctcc aggattatcg gtgatacacc ggctcaccag
     2161 gcactgcgac tggtccgcat ggccaccgga ttcggccgac gcgccgccac gaccggcatg
     2221 cgcaacgtgg ccgaatacct ggcgcatgaa cgccgtgacc tggtgccgcg cgctgaagcc
     2281 gaggatttct accggggcgt cgaacgcctg cgtgaagacc tggatcgcct cgaagcccgc
     2341 acgggccaac tgggtgaagc cggtgttgag gacgaagtac aacgatgaaa ctgcgcgttc
     2401 tctcccggct catcgaaatt cagcgcgtgc tggtgcgcca cgggttggac gaattcgtcc
     2461 gcgccaccca cctgtatcgg ccgctgcgat tcatattcct gctctcgccc tggacctggg
     2521 cggtgcgccg tggcgacgcg ccacgcgctg aacgcctgcg cctcgcgctc gaagagctgg
     2581 ggccgatctt cgtgaagttc ggccaggcgc tatctacccg ccgtgacctg ctgccgctgg
     2641 acattgccga cgagctggca aagctccagg atcgtgtacc gcccttcgat ggaaaagtcg
     2701 cgcgcgccgt catcgaggcc gcctatggcc gcgccgcgga acaggttttc tcggtgttcg
     2761 aagaagagcc tctggcggcg gcctcaatcg cccaggtaca cacggccgaa ctacgcgccg
     2821 aagtgccgct gaacggcgcg aagacgcgcg aagtggtggt gaaggtgctg cggcccggaa
     2881 tgcacgccat catcgcgcgc gacctcgagg tgctgcgggc gctggccgcg ttcgcggaag
     2941 ccaactggga aggcagccag cggctcaagc ccctggaagt ggtcgccgaa tacgaaaaaa
     3001 ccatcgtcga tgaactcgac ctgatgcgcg aagccggcaa cgcctcgcag ttgcgccgca
     3061 atttcgagaa ttcccctctt ctatatgtgc ccgccgtgca ttgggatttc tgccggcccg
     3121 gcgtcatggt catggagcgc atccgcggcg tgcagatctc caacatggcg cggctgcgtg
     3181 aggccggaac cgacatccgc aagctggcgg aaaacggcgt cgagatcttt ttcacccagg
     3241 tgttccgcca caacttcttc cacgccgaca tgcacccggg aaacatcttc gtgctcatcg
     3301 acaacccggc gcagcccaag tatgccgcgg tggatttcgg catcgtgggc acgctggatc
     3361 cgcgcgatca gtactacctc gccgaaaatt tcctggcggt cttcgatcgc gactatcgca
     3421 aggtcgccac gctgcatctc gagtcgggtt gggtaccgga aggctcgcgc gtcgacgaga
     3481 tggaatccgc catacgcacc gtttgcgaac cgatcttcaa caagccgttg aaggaaatct
     3541 ccttcggcac cgtgctgctg cgtctgttcg agatttcgcg gcgtttcaac gtatcgatac
     3601 aaccgcagct catcctgctg cagaagaccc tgctcaatat cgaggggctg ggccgcgacc
     3661 tgtatcccga cctcgacatc ttcaagaccg cgggccccat catgcgcgag tgggtgcgcg
     3721 agaagatgag cggccgcgct ttgctggaga atgcgcggac gcaactgccc gagatcctca
     3781 ccagcctgca atcgctcgca ccgctggtgc gttcggcggt gcagcgcgcg caggacggcc
     3841 ggctgcgcct cgccgtggaa gcacccgaga tcaatgcgtt gcgaacggaa atccggcgca
     3901 cgaatcgccg acgtgacaaa gtgacgatcg ccgccgtgct gctgctcggt ggcctcgtgt
     3961 ggatggcggt cgggcgcgac cccgcctggg cggggtgggc actctcgggt gccggcgcgg
     4021 catggttgtt aggattgctt cgccgctgag tctccctcgg aaaataccta gtcaagttcc
     4081 ggtgccaagg ccaattgcgg cactgcaatc atcggagtag tttgacaaat catggtgatc
     4141 cccgagagcg ctgaaccgag ccgcaacctc gcgcagttcg tcgctttcgt cgcgctggcc
     4201 gtcggcctgt tcgttttggc cggctgggcc ttcggcatgg atcagctcac caacatcgtg
     4261 ccgacctggc cgagagtggt gcggctcacc gcgctggctt tcatcctgtc gggcttcgcc
     4321 ttgtggctgg cgaccatcca gaaacgggtg ccggccatca tcgccgccgg gctgctgact
     4381 gccatcgggg cgatcgtgct cctcaaatac gccaccggct gggacgtcta cctggaccag
     4441 ctctcgctgg cgcagatgcc gcaggttctg gacggcaata ccccgccgcg catggccccg
     4501 gcctccgccc tcgccttttt cctgctgggc gcgtcgctgc tgttcgcgca gcgaccgcgc
     4561 ggtgcgttga tccaccaggg cctggcggtc gtggggatgg tcctgggatg gctgggccta
     4621 tcgcgataca tgttcggcgg tgagccgctg ttcccattcg cggacatggc ggtgcatacc
     4681 gcgaccctgc tgctgatgct gaccggcgcc acgctcacgc tgcgtcccga cgccggcgtc
     4741 gcacgcctgc tggcgagcgg cggagtcggt ggcgcgatgg cccgccggct gctccccgcg
     4801 gccatcatcg tgccgctggt ggcgggctcg ttgacgctgt acttcgaacg gcatcacaca
     4861 ttgagcttcg cgacggcagt ggcgtttttt gcgctcgcga gcgtcgtggt tttcgtcgcc
     4921 ttcgtctgga tcaatgcggc gcgtggcgaa cgcgcggact ccctgagacg cgcggccgaa
     4981 cgcgcgct
//
LOCUS       DASQJS010000361        33250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2110782, whole genome shotgun sequence.
ACCESSION   DASQJS010000361 DASQJS010000000
VERSION     DASQJS010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 33250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33250
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110782"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2442)
                     /locus_tag="VEW08_14170"
     CDS             complement(<1..2442)
                     /locus_tag="VEW08_14170"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYJ41934.1"
                     /translation="MFASPASGTLVNNGEPPPADWLDGKDAPRFRVDAIQKVTGDKTF
                     TRDFRARDIPGWPKEQSHAFLIRVARADRTFEGVDLSTLGDDLLPDRLVLAEDLERDG
                     IRMPHADFYGEWFLVPRGKTPHLLGEPVALLIYKDFDRFDAAKRRIQFDEGVVRYGAQ
                     TGPNPPPNYGAARYVRIEGATPESPDENSPIRDSIIFAGFRDDQPQWPRPSRTGDPMA
                     RGMAAAARIDTDFADAGDDTLVIRRSYTSQSIDASAMETDNGNVWYDPAAQVMSMMIA
                     TQSPFEVAERAAEMLSKSKFAAKKVNLKCGHTVGYGTKDHHVFPLFAALAGLYGEGRP
                     VRLANDRFEQFQMGLKRHAFWMKYELLVDRKTLRFRAMRGEFKCDGGGRANYSAVVGM
                     VGATAAQSIYYLPRSDFSVATLASRAVEGGSMRGFGTLQTMSATEMLVDETAQALGID
                     AIDLRLKNVFRTGMKNTQGAVPIGSLRNDEILERAKAHPLWTERATRKKKYETANPGK
                     RYGVGFAHVQKDYGTGGEAAIATLEVDRDGRVTLRHVAHEIGTGTTTSQPIIAGRILG
                     RVPDRTFYGVVEWPEMPLTASEQQFTMSQAMEDKLGRNPRWTPSFTSPMSASNSAFFL
                     GHATRESALALVRFGLWPAALRLWGMSAASAAPLGPQDLRAVDGKLVAGSLEPLSFAR
                     VAAKAHELGLVTGVSVHTFNRWDWAEAEFDIPEAGRLRLPIDALALKYGEGASPARKA
                     KMRNGGYHFIDRAAVFYPPTQLNNAGTRYYAPMATLVEVAVNLGTGKVEVLSHHSILD
                     PGPPIVAQLISGQVQG"
     gene            complement(2511..3410)
                     /locus_tag="VEW08_14175"
     CDS             complement(2511..3410)
                     /locus_tag="VEW08_14175"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HYJ41935.1"
                     /translation="MSAITEQGTLKFSIALTVLLGVLGVASGLATGSQAIIFDGMFSF
                     VDVLPTIVSLLVVKLIARGTSQRFQFGYWHLEPLVGVLRDSILAVACIYAAVDAVNSL
                     FSGGHEVEYGLAAWWAGILCAIGLAMTMVLRRRARVLQSPMLELDARSWAVSTVLSLA
                     LLIGFIFAQALAGTRWQGWIPYLDAIALLSMALIMLPVPLMGLWRSISDVLQVAPDEL
                     DARVRAVMDRVVKERAFLDYSSYVAKVGRGRFVEIHILVPSESRIDIAMADEVRRDVS
                     DRLNAASPTFWLTVDFSADRRWI"
     gene            3634..3918
                     /locus_tag="VEW08_14180"
     CDS             3634..3918
                     /locus_tag="VEW08_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41936.1"
                     /translation="MSSRLELTPEQENKLVPLFQNRASELRQSQALLEKATTPQQQKE
                     VLRDVEQAGKEFNTQVERVLTPSQQDEWREFISDLREKAKERIKDKRESR"
     gene            complement(3919..6360)
                     /locus_tag="VEW08_14185"
     CDS             complement(3919..6360)
                     /locus_tag="VEW08_14185"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ41937.1"
                     /translation="MSSGKKAGEMTTRAAPIRRKLSTISLVSTGAALLLTTAVFLAGE
                     IVAARDSSLQQLRILSEAIATNSTAALAFDNPEDARAVLAAFKSDPHIIAAALYGSDG
                     QLFVAYPELAPAGALPEATGPPGYNIAGSALIGVARVREGARELGTLYVRSDFSAVYD
                     RLAFYALVAAIAIALALFAAWAISRRLQKELSTPILELAATARLVSDRHDYGVRARLA
                     GIIELDDLTNAFNHMLMQTEQNEKRMSAQVNRLALLQQITHSIGSRHDLKSIFQVVLR
                     SLEEHLPIDFGCVCLCNAATGMVVVESVGAASETSCKSLGLALGSAVPVGKNGLYRAV
                     NGTLIHEPNTAGVDSPFTRRFADAGFHSLVISPLAVESNVFGVLVAVRREKEAFSSGD
                     CEFLKQLSEHVALASHEAQLYGALQRAYEDLRHSQQTVMQTERLRALGEMASGIAHDI
                     NNAISPVALYAELLLSQETQITEAGRGRLVTMRRAIDDVAGTIDRMREFYRPRELTRA
                     FTKLDIHSAINQVLDLTQPRWRAQPQQRGIVIELLKDFAPKLPNVMGDEVELRDALTN
                     LVFNAVDAMPEGGTLTLRTRPFVDGDGRVTVRVEVGDSGVGMDEDTQRRCIEPFFTTK
                     GERGTGLGLASVYGMLQRHSAVLEVDSASGKGTTMRMIFPAFTEDASAARSQLASHVS
                     LRKLRILVIDDDPILIRSLLEVLEADGHAVTTALGGEEGIAAFAAARGTEEQFEVVLT
                     DLGMPRVDGRKVSAAIKHSSPTTPIILLTGWGQRLLDEATLPENIDRVLSKPPRLNLL
                     RAALLELANPRDPGL"
     gene            complement(6335..6922)
                     /locus_tag="VEW08_14190"
     CDS             complement(6335..6922)
                     /locus_tag="VEW08_14190"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HYJ41938.1"
                     /translation="MAPLQLRPSARGTALRRWLLGAVCVVAAGVCSAALSAPPVAEYQ
                     VKAAYLFNFGQFVEWPLQAYDSPGAPFVIGVVGDDPFGKILDDVIAGESLGGHPLVVR
                     RFKNPEDISACNILFIGRSETARLEETLKVLQGRSVLTVTDIAGAEHRGAVIALVNEN
                     NRIRMRINVATAKANNLVISSKLLRPADVVGKEGG"
     gene            complement(6929..8929)
                     /locus_tag="VEW08_14195"
     CDS             complement(6929..8929)
                     /locus_tag="VEW08_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ41939.1"
                     /translation="MTSRDGPAWLLICGALIPGSALANNSSAGHLDALKRMSFEELMT
                     VEITSVSRTEETLRDAAAAVAVISPEIIRRSGATTVPDALRLVPGLHVGEQTASSWAV
                     SARGFSSINSEKLLVLSDTRSIYTPLFSGVNWTVQDYLLEDIERIEVIRGPGAALWGS
                     NAVNGVINITTRSARDTHGTRIEAGAGTFDRAWLEGRYGGETSGGVNFRVYGKYFERD
                     ATFAPTLDTEDSWNLGHVGFRTDWDGSGHDSFTVQGDAYRANVGQLAAAINIIGRPGP
                     PPPLNVDLSGGNILARWRRSAEDGADMQLRAYYDYTRRDDPSFLDTLHTFDIDLQRRF
                     NAGSSHEILWGAAYRHTANTNQGRVVFAVEPEKSTDQLFSGFIQDQISLGRELRVTVG
                     TKLEHNDFSGFELQPSVRLAWLPRDNHTLWVAISRAVRVPTRIERDIAIDLTDPAGNP
                     VGRLLGNEDFRSELLVAYETGYRWQPLERLSFDLALFYNDYDRLASLEIGEPFIAADG
                     RTVIPVINENLTSGRTYGAELLVEWQPVESWRLSASYSHIDMSLDAEGQDLNRGEWLE
                     GATPRDIAGLRSLLTLGTSVEIDAQLRYQSRIERQPMVVSGESMDGFTELDLRLGWQT
                     TANWSLSLVGQNLLHDNHVEFGSIDRRGALERAAYLKATWRN"
     gene            complement(9357..10424)
                     /locus_tag="VEW08_14200"
     CDS             complement(9357..10424)
                     /locus_tag="VEW08_14200"
                     /inference="COORDINATES: protein motif:HMM:NF015187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HYJ41940.1"
                     /translation="MKYGHYLYALAAFALADAALGANRPAGYVTICNENKTCSVAAST
                     NVAFGRADQFLFKTLSGSFVCSSATFGGRIAGGVNECSVPTGSAPPPPPPPPPPPGGG
                     TADLGYYPGCAMPTNTQTVQLTATQVVPAGTVFDGQNKVYNLSGGSQSEGQPAVFDVQ
                     HGATLKNVIIGTLAADGIHCLGSCTLDHVWWQDIGEDAATALGPAGTVMNITCGAAYN
                     GSDKTFQFNGRGELRISNFYAANAGKLVRSCGDCTGNGGPRKIWVTNVITRDVPTIVG
                     INSNFGDVATIRNLTLNNSGTTKTKICQVYKGVVKGNGSTSALGVEFNTPNCQVSQAD
                     VTLLPPSQMNTSACAGSCPIP"
     gene            complement(10693..11763)
                     /locus_tag="VEW08_14205"
     CDS             complement(10693..11763)
                     /locus_tag="VEW08_14205"
                     /inference="COORDINATES: protein motif:HMM:NF015187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HYJ41941.1"
                     /translation="MKNVQVLVALGAIALAGSASAANRPSGWTTICKEGQTCTVAANT
                     TVAMGRADQFLYKVLSGSFACSSTTFGGKIAGGVNECSTGPGTAPPPPPPPPPPPPTG
                     GTDLGYYPGCAMPTNTQVVQLSATQVVPAGTVFDGQNKVYNLAALGDGEGQPAVFDVQ
                     HGATVKNVIIGPLAADGIHCLGSCTLDHVWWQDVGEDAATALGAAGTVMNVNCGAAYS
                     ANDKTFQFNGRGELRISNFYVANVGKLVRTCGDCTGNGGPRKIWVTNVIARDVTSTVV
                     GINSNYGDVATIRGLTLANSGTSKTKICQVFKGVVKGSGSSTALGVEFNTPACNVSQA
                     DVTLLPPSVMNTSACTGSCPIP"
     gene            12009..13079
                     /locus_tag="VEW08_14210"
     CDS             12009..13079
                     /locus_tag="VEW08_14210"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYJ41942.1"
                     /translation="MPKLLLFIACALLGLRSAQAADYPAPAEGDFVLRDFHFSNGESL
                     PELKIHYRTLGSPKKDAKGIVRNAVLIGHGTGGSGAQFIRPEFAGELFGAGQPLDATR
                     YFIVLADGIGHGKSARPSDGLRAKFPQYGYTDMVEAQYRLLTEGLKVNHLRLVMGTSM
                     GGMHTWVWGERHPEFMDALMPLASLPVQISGRNRIWRKTLIDAIRNDPAWQGGNYTAQ
                     PPSLSTAISLIFFMGSNPVLRQEQMPTFARADEVLAESVANAIRSQDANDILYQVSAS
                     WDYDPAPALERIRAPLLAINSADDLINPPELGILEREIKRVKRGEAILIPLSPQTRGH
                     GTHTFAALWKQHLVKLLDESAQ"
     gene            complement(13088..13801)
                     /locus_tag="VEW08_14215"
     CDS             complement(13088..13801)
                     /locus_tag="VEW08_14215"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HYJ41943.1"
                     /translation="MNRAGVIVALAGVLMFGSAAGIGAGLNESDRTPWHTLELSLLET
                     NEALRVAHAALDDTQLMNLEAFSNLGGTNVPYVRPDRSRPSFCGDCELQVGLGGTYHS
                     FEATGGEVIPVTLTWDRGRWEFGVFHFGEQTSTDNSENVERLVARPYWGASMSRRFKL
                     FERGPLRAIFGFGLSYRTETDVLSATHWNFSSQFGLRYQLSQFPAVVELSARHWSNGG
                     VRTPNRGQDFTILTVRFDR"
     gene            14011..15111
                     /locus_tag="VEW08_14220"
     CDS             14011..15111
                     /locus_tag="VEW08_14220"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ41944.1"
                     /translation="MTRTDSKNSRLERIAASPRWDGVRFRNSSPISRGDPTVAMPSMS
                     EFICGGDRRVPQAPLPSRNPLDIWTRPAGSGLRATWLGHSTVLIEIDGWRVLTDPVWG
                     PRASPSRFIGPKRFQPVPVALRELPPLDAVIVSHDHYDHLDYTTMRLMRKASVPIITS
                     LGVGAHLESFGIAPERIVELDWWESHRVPGTDLTLHAGPSQHFSGRGLKDGNKTLWSS
                     FVIQSQRHRVFFSGDTGPTAEHAAIRDRLGPFDLVMLEVGAWHPAWGNIHLGPANALA
                     AHTLLGGGPLLPVHWGTFNLALHPWDEPVETLLQLAPKTSARLILPRLGEPVEPAHDF
                     SVTPWWRGIDLDARNPRRGSTRMPKAMPFPPD"
     gene            complement(15121..15819)
                     /locus_tag="VEW08_14225"
     CDS             complement(15121..15819)
                     /locus_tag="VEW08_14225"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HYJ41945.1"
                     /translation="MKVVRRSSDRGHADHGWLKSYHSFSFADYFDEDHVEFGPLRVLN
                     EDRVTGGQGFGTHGHRNMEIISYVLEGELTHQDSIGTRSTIRPGDVQRMSAGSGVRHS
                     EFNGSQTNKLHFLQIWIQPNVADIPPSYEEKRFEAADKQGRLRLIASSNGEAGSVLVH
                     QDVRVYSGLFDGAEHASLPLAAGRRVYVHVARGSVHANGIELDAGDALKLTEEAVLEI
                     AQGSRSEVLVFDLP"
     gene            15855..16874
                     /gene="gnd"
                     /locus_tag="VEW08_14230"
     CDS             15855..16874
                     /gene="gnd"
                     /locus_tag="VEW08_14230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HYJ41946.1"
                     /translation="MKENRVGFIGLGRMGANMVRRLSAAGFQCITFDANAAAVQALLA
                     PGITGAATLQDFVAKLAAPRVIWLMLPAAVVDQELKSLQPMLTAGDTVIDGGNSYYRD
                     DLRRGAELAKAGIHYVDVGTSGGIAGGENGYCLMIGGEKAAVDSLEPLFAALSPGGQS
                     SYNNSDKQNTAQRGYLYCGPYGAGHFVKMIHNGIEYGVMAAYAEGFNILRNADIGTRP
                     REKDAETTPLQHPEHYAYEFDVAQIAEVWRHGSVVRSWLLDLTARALSEDASLAAFAG
                     RVSDSGEGRWTVAAAVDEGVPAPVITAALYSRFESRGNADYGDRLLSAMRKQFGGHVE
                     KKGGN"
     gene            complement(16884..17333)
                     /locus_tag="VEW08_14235"
     CDS             complement(16884..17333)
                     /locus_tag="VEW08_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41947.1"
                     /translation="MGFQPDSSGSRAFYSLFINAARAEVEPMGEPRNPAHVLGVGSEI
                     ASVNGPVFAAYFASSRERFDAQPNYKAGLFVLVDGAVYAVTPEQIDTSIRRGFSTREF
                     TLLRGKRPQRSVVYPWPWYREPFVSGGSGVRSGDFLREMSAMVREYG"
     gene            complement(17615..18889)
                     /locus_tag="VEW08_14240"
     CDS             complement(17615..18889)
                     /locus_tag="VEW08_14240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ41948.1"
                     /translation="MWPFSKNSAGSIRSSEAYWLLRNGIGDASPALTESIDCDIAIIG
                     AGITAALTADALIATGKRIVVLDSRDVAQGSTSASTALLQYEIDTHLTDLVPLLGAER
                     AMRAYRACAASFTELEARFPELLPEAGYERRPSLYLAADEKAVDPLLVELKARRDIGL
                     ACEWLDADELRRRFGCLRPGGILSALGAQVDPFRLTRAILSACRRHGVQLFARTRVTQ
                     IEEQGDAFVLRTAAGHSVTAAHVAVCAGYESLDFLPSGYADVHNTFALVTEPLTGCER
                     MATLPLIWESARPYFYMRGTSDGRLLMGGADVPFKNAAARDLMLPRQVRRLQQQYQTL
                     FGSELPPVAYGWAGSFAETHDGLPLIGRVPGMSPRLQFALCYGGNGITYSVHAGDIIR
                     AGVEGRVHALDDVFGFGRVGTGSSAGRSAVPA"
     gene            complement(19007..19885)
                     /locus_tag="VEW08_14245"
     CDS             complement(19007..19885)
                     /locus_tag="VEW08_14245"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYJ41949.1"
                     /translation="MKITNYSFSSTLIGAAVAAVLLASCASTQRPAGSAEVRAKLTAL
                     QGDTSLANGAPVAMKDAEGAVVLAEMIEKDLALAAHRVYIADRKVETARALAVTHVAE
                     QQRTALTEQGEKARLDSRTREADVAKNDALVARAENAEQKMIAGQARDSADAAQQQAL
                     ELQRQLEILQARPTDRGLVLTLGDTLFATGKSEIKSGATVNLDRLTAFMNEYPQRTAA
                     IEGYTDSMGSEDMNQSLSQRRADAVKGYLVGRGVNSARLSSSGRGENSPVADNESAAG
                     RQQNRRVEVVISPAAP"
     gene            complement(19882..20286)
                     /locus_tag="VEW08_14250"
     CDS             complement(19882..20286)
                     /locus_tag="VEW08_14250"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HYJ41950.1"
                     /translation="MYSPSKRTSVSRRRLRIALPVLVLVASTAFARPPVPTTNLQAAQ
                     QAIANAERVDAATLAGVELGEARGKLAAAQRAVEEKEMVVAQQFADEARAGAELAAAK
                     SGAAKAVAVNKDIERSTATLIDEMQRKTGESR"
     gene            complement(20438..20881)
                     /locus_tag="VEW08_14255"
     CDS             complement(20438..20881)
                     /locus_tag="VEW08_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ41951.1"
                     /translation="MSDAPPRILIVDDDVEIAKSLAALLETVGAYETRIVHCAADAVA
                     VAVEHAPAIVFLDIELPDMSGYDVAKLLHQHADLQAVRLIALTDNIEHPGREGARAAG
                     FERYLVKPVTAIELQKVLRRSVSIGAGLSVAARNGKREPIYRGLP"
     gene            complement(20878..21699)
                     /locus_tag="VEW08_14260"
     CDS             complement(20878..21699)
                     /locus_tag="VEW08_14260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYJ41952.1"
                     /translation="MPLLIKSMLGSVATLLHRQDPPSTPGIVAPLHLPDARADEIAVI
                     SHELRNSLAVVRNAARLLRHPVGVSGVDGARILIERHVGQMTRHIEDLLDASHRDVHK
                     RALCRTHVDLRAIAEFAVAAIAPDLARRGHHFVLHLPAEPLWAEVDAARLEQVFSNLL
                     INAAKYTPDGGEIMLTMESLGQSISICIRDSGIGIEPAVLLRVFDMFAQADASAPCAE
                     GGSGIGLAVVRDVVEMHGGSVHAASAGLGLGSEFTVLLPSLWAQPTALTLVPVTR"
     gene            21978..22712
                     /locus_tag="VEW08_14265"
     CDS             21978..22712
                     /locus_tag="VEW08_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYJ41953.1"
                     /translation="MNLKPEPRQNHILDALPPLERERLFPHLKLVPMPLGMVLYESGA
                     ALRHIYFPTNSIVSLLYVMQDGASAEIAVVGNEGAIGVSLFMGGETTPSRAIVQSAGH
                     AYRLTGKRLKQEFNRHGEMLHILLRYTQSLITQMAQTAVCNRHHSLDQQLCRWLLLSL
                     DRLSGDTLQMTQELIANMLGVRREGVTEAAGKLQKLGVIKYVRGKITVLDRPQLESLS
                     CECYAVVKRESDRLLAPHPLSHSAEA"
     gene            complement(22651..23385)
                     /locus_tag="VEW08_14270"
     CDS             complement(22651..23385)
                     /locus_tag="VEW08_14270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ41954.1"
                     /translation="MNTAVVPDGTHLQFVAIVANELRHPLVPIRNAAALLREESLDAA
                     TIALAAQIIEDQVSGMNRLIADLIDFARMRTDAVELQLARALLSELLERVLASGALVA
                     SERGHGLSVSVSPDPVYLNVDVVRIAQALHNIIGNASKFAGTHDPTHIHARREGGQAV
                     IVVSNLKLGVAAAEIDMIFDLEPGLGLGLYLARYLVEAHAGTLSAVVAGADQGSEFTV
                     RLPCEAPMPQPNDSADAAPAGDPIPA"
     gene            23611..24399
                     /locus_tag="VEW08_14275"
     CDS             23611..24399
                     /locus_tag="VEW08_14275"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYJ41955.1"
                     /translation="MPIKRAPSENLLLAAMSNEDRLRLLGDSVAIDLRLDETLSHSDD
                     RIHHVYFPIDGFISLITPGIGRAQLEIGLVGNEGMLGVPLLLGINVSPLRALVQGSGH
                     SWRVDAAHFLQVIESHASIRTALNRYLYVFLAQLMQTATCTRFHLVEARLARWLLMTR
                     DRAHSSQFHITHEFLAYMLGVRRVGVTSAASALQRRKLIRYKRGDIAVLDVRGLELAA
                     CPCYAIDKKIYGRVLGKGRGSRLHAGRDPAAIRLAARIAGAISC"
     gene            24519..33098
                     /locus_tag="VEW08_14280"
     CDS             24519..33098
                     /locus_tag="VEW08_14280"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HYJ41956.1"
                     /translation="MHPFLSEIIEHLSPGTHWSDDQILREEIFSIERLEEHARSLAAA
                     QPVGSIPGFGRSLSTRLRSNEKVLLAAYRSIATAVDEGRSITPAAEWVLDNYHVVEEQ
                     IREIREDLPPQFYRQLPKLTAGPFAGFPRVFGIAWAFVAHTDSRFEPETLRRFVRAYQ
                     SVEPLTIGELWAVAITLRIVLVENLRRVAQRIVTSRLARQEADAVADRLLGVNGQRVD
                     PNALRTWRARSPTLKSAFAVQLVHRLRDQDPKITPALLWLEEELAEHETSSERLLQEE
                     HLRQGASNVTVRNIITSMRLMSDVDWAEFFEHVSLVDDVLGTSPDFAAMDFATRNLYR
                     NAIEEMGRGSKLSELEIAKRVLATAAAATSPRERDPGFHLIAEGRRKLEREIDFHASW
                     RDWPSRIAFTVGARDYVAAIAFTSALVLSIPLSLLQQAGIHGGMLWLLGVLGVVPAVD
                     IAVALINRAVTRGVGASRLPGLALRDGVPAELRTLVAMPVMLSSRASVEEHLHRLEIH
                     YLASPDDQLHFALLSDWSDADTEQKATDESLLDIAVLGVARLNRRYGPAAGGERFLLL
                     HRRRLWCETEQRWMGWERKRGKLQELNRLLRGATDTTYVTSTGASPWVPDGVRYVLTL
                     DGDTRLPREAPRRLIGKMAHPLNRPRFDARMGRVVEGYAILQPRVAFSLPTSSEATPF
                     QRAFSGAAGVDPYAAAVSDVYQDLLGEGSFAGKGIYDVDAFEAALAGRVPESSMLSHD
                     LFEGIFARAGLASDVEVVEDFPSRYDVASLRQHRWVRGDWQLLPWILGRRDVGAAEHG
                     RGHSRLPLIGLWKMLDNLRRSLSAPTAVAALVAGWTLPANIAIPWSGFILLSLALPTL
                     LPLFAAILPRRSTVTLRSHLRALRLDFGIALAQTGLLISMLAYQAWLMCDAIVRALWR
                     MCVSHRHMLEWTPADMLSSARSDLPSFYRRMAHSVILTVLIAAGLAAVSAGSLPVIAM
                     PFLLLWFTAPLLAWRVSRTPPAVAKSALSTVQQRDLRLIARRTWRFFETFVTAEDNHL
                     PPDNFQEDPRGVVAHRTSPTNIGLYLLSTVAARDFGWCGLRDALDRIEATLGTMVRMH
                     KCRGHLYNWYDTRDLRPLDPRYVSSVDSGNLAAHLITLAGAFREWQKVPAPPASAVDG
                     LADALDLAREALRTFQFAPGLTITRGLLETAFSDFEASLRPRTMPLDPQTDALRDAAE
                     RAGTLVDMVRTLASESDPERSAELVYWVEAARRTIDSWRSDLLARDPAGFIVEHLESL
                     ANIALQLASAMEFDFLLDSQRKLLSIGYRATDGTLDDSCYDLLASEARLASFIAIAKG
                     DIPARHWFRLGRTVTPIGAGAALVSWSGSMFEYLMPDLVMRAPGGSLLAQTSRLIVQR
                     QIAYGEELGLPWGVSESAYNARDLELTYQYSNFGVPGLGLKRGLSENKVTAPYATGLA
                     AMIDGEAALANYTALAEIGARGPFGFYEAVDFTPSRVPEGETQVMVRAYMAHHQGMSI
                     VAIANALLDGRMRERFHSDVSVQATELLLQERTPRDVSVAHPRAEEVGTAARFADPQV
                     PQVRRIRSPHDSAPQTHLLSNGRYSVMVTAAGSGYSRWNDLAITRWREDVTRDDTGSY
                     LLMRDVESGRVWSAGYQPCAGQPGSYEVSFTEDRAEIIRADGDVISTLEVQVSPEDDA
                     EVRLLSLTNGSMREREIEVTSYAELVLGSQASDLAHPAFSKMFVRTEFLARQNLLLAN
                     RRRRAPDEPEVWASHHAVVEGVVVGAPQHETDRSKFLGRGRELRAPLALLEGRALSGT
                     VGTVLDAVFALRYRLRIPAGGTARIAFWTCVAGDRAQLLEIADKHRDPNARTRTATLA
                     WTQGQVQLRHFGIDASQANVFQQLAGHVLFASSAARASADKIQRGAAGPAGLWAQSIS
                     GDLPIVLLRVDDMDDLVTVRQLLQAHEYWGIKRLAVDLVILNERGASYVQDLQVAIEA
                     AVRTSLARPRIAGADTRGKVFVLRTDLITPETRGLLRAVARVELSGKRGSLADQVERM
                     QPVPATAPRLPRRALVSSVTVADPEDTRRLEFFNGIGGFAAQGREYRISAPDGQNTPQ
                     PWINVVANRNFGFQVASDGGGYTWSRNSRENALTPWSNDPVTNRPGEAIYLRDEESGE
                     LWTPAPAPIRHENAQYSCAHGMGYSRFEHVSHGIELELTMLVPLEDPIKICRLRVRND
                     SPRRRSISITAYVEWVLGPSRTAGAPHVFTEQDAVSGALLARNPWNTEFPGVAFADLR
                     GAQTEFTCDRSEFLGRHGTLEAPAALVSGAQLAGRAGAGLDPCAALRTRLELEPSAAT
                     EIVFLLGEAVGTDEARALIATYRGADIEKLLGKVREHWDEVTGHVQVKTPDRAFDIML
                     NGWLLYQTLACRTWARAGFYQASGAYGFRDQLQDAMALGIARPQLLREQILRAASRQF
                     PEGDVQHWWLPHSGQGVRTHISDDRVWLSFVTAHYIALTGDRAILDAQVPFIEGPPLP
                     RERHDAFFTPSVGEHTATLFEHCRLGLEGALYMGQHGLPLIGTGDWNDGMNRVGEHGR
                     GESVWLGWFLHATLHAFAPVAQARGELALATAWLGHATRLRAALEQHAWDGEWYRRGF
                     FDDGTPLGSAHNDECQIDAIAQSWGVISGAANPARTTRAMAALDAQLLNRDPPLAMLF
                     TPPFEKSAQEPGYVKAYPRGVRENGGQYTHAAVWTSIAFAMQGNAERAMEVFNMLNPV
                     KRTTTRADVQRYKVEPYVVAADVYSEAPHAGRGGWTWYTGSAGWLYRAGLEWILGFRV
                     RENRLTLVPCVPSEWPGFTIHYKHRGTPYEIIVDNQPHPHALPLLVIDGDVQNAGRNT
                     VDLIDDGAKHTVHMMWRAATAQEDFAATRSS"
BASE COUNT         6003 a        10867 c        10427 g         5953 t
ORIGIN      
        1 gccctgcacc tgacccgaga tcagctgcgc gacgatcggc ggccccggat cgagaatcga
       61 gtgatgcgac agcacctcga ctttgccggt gccgagattg accgcgactt cgacgagcgt
      121 cgccatcggc gcgtagtagc gggtgccggc attgttgagt tgcgtcggcg ggtagaacac
      181 cgcggcacga tcgatgaagt ggtagccccc gttcctcatc ttcgccttgc gtgccgggga
      241 agcgccttct ccatacttga gagcgagcgc gtcgatgggc aaccgcaacc gtccagcctc
      301 gggaatatcg aactcggctt ccgcccaatc ccagcgattg aacgtatgca cgctgacgcc
      361 ggtgacgagt cccagctcgt gggccttggc ggcaacgcgt gcgaacgaga gaggttcaag
      421 cgatcctgca accagtttgc cgtcgacggc acgcaagtcc tgcggcccga gaggcgctgc
      481 cgacgccgcg ctcatccccc acagtcgcaa cgccgccggc cagaggccga aacgcaccag
      541 cgcaagggca gactcacgtg tggcatggcc caggaaaaac gcgctgttcg atgcgctcat
      601 cggcgaggtg aatgacggag tccagcgagg gttccgaccg agcttgtctt ccatcgcctg
      661 cgacatggtg aactgctgct cgctcgcggt gagcggcatt tcgggccact cgaccacgcc
      721 atagaaagtt cgatcaggaa cacggcccag gatgcgcccg gcgatgatcg gttgtgatgt
      781 ggtggtgcca gtaccaattt catgcgccac gtgacgcagc gtgacgcgcc cgtcgcggtc
      841 tacctcaagc gtcgcgatgg ccgcttcgcc cccggtgccg tagtcttttt gcacgtgtgc
      901 aaagccgacg ccgtaacgtt tgccaggatt ggccgtttcg tacttcttct tgcgtgtcgc
      961 gcgctcagtc cacagcggat gcgctttcgc tcgctcgagg atctcgtcat tgcgcaagga
     1021 gccgatgggc acggcaccct gggtgttctt catgccggtg cgaaagacat tcttcaggcg
     1081 cagatcgatg gcgtcgatgc cgagtgcctg cgcagtctcg tcgaccagca tctcggtggc
     1141 cgacatggtc tgcagcgtgc cgaagccacg catcgagccg ccttcgacgg cgcgtgacgc
     1201 aagcgttgcc accgagaaat cgcttcgcgg cagatagtag atggattgcg ccgcggtcgc
     1261 gccgaccatt ccgacaaccg ctgaatagtt cgcgcgtccg ccgccgtcgc acttgaactc
     1321 ccctctcatg gcgcgaaacc tgagcgtctt tcgatcgacc agcaattcgt atttcatcca
     1381 gaacgcatgg cgcttcagcc ccatctggaa ttgttcgaaa cgatcgttgg cgaggcgaac
     1441 cggccggccc tcgccgtaga gcccggccag ggcggcgaac aagggaaaca cgtgatggtc
     1501 cttggtgccg tagccgaccg tatgaccaca cttcagattg accttctttg cggcgaactt
     1561 cgacttcgac agcatctccg cagcgcgttc cgcaacctcg aacggcgact gcgtcgcgat
     1621 catcatgctc atgacctgcg ccgccgggtc gtaccagacg tttccgttat cagtctccat
     1681 cgccgaagcg tcgatcgact gggaggtgta cgagcggcgg ataaccagcg tgtcgtcgcc
     1741 ggcgtcggcg aaatccgtgt cgattcgagc cgccgcggcc atgccccggg ccatcggatc
     1801 gcccgtgcgc gacggtcgcg gccactgcgg ttgatcatct ctgaacccgg caaaaatgat
     1861 cgagtccctg atcggcgagt tttcgtcggg tgactccggc gtggctccct cgatacgtac
     1921 gtagcgcgcg gcgccgtagt tagggggtgg gttcggcccc gtttgcgcgc cataacggac
     1981 gacgccctcg tcgaactgga tgcggcgctt ggccgcgtcg aatcgatcga agtccttgta
     2041 gatcagcagc gcgaccggtt cgccgagcag gtgcggagtc tttcccctgg ggacgagaaa
     2101 ccactcgccg tagaaatcgg cgtgcggcat cctgataccg tcgcgttcga ggtcttcggc
     2161 gagcaccagc cggtccggca gaagatcgtc gccgagcgtg gaaaggtcga cgccctcgaa
     2221 agtccggtcg gcgcgcgcaa cacgaatcag gaacgcgtgg ctctgctcct tcggccagcc
     2281 gggaatgtcc cgggcgcgga aatcacgggt aaaagtcttg tcgccggtga ctttctggat
     2341 ggcatcgacg cggaagcggg gcgcgtcttt cccgtccagc caatccgcgg gcggtggttc
     2401 gccattgttt accagcgtgc ccgaggccgg agaagcgaac atgctgattt gaatcgttat
     2461 gcccgcgaca actccggact tgaagaaatc acgacgtgag atcggatggc tcatatccat
     2521 ctgcggtcgg cactgaaatc gaccgtcagc cagaacgtcg gcgacgccgc attgaggcga
     2581 tctgaaacat cgcgccggac ttcatctgcc attgcgatat cgattcggga ttccgacggc
     2641 acgagaatgt ggatctcgac gaatctcccg cgccctacct tggcgacgta actcgagtag
     2701 tcgagaaacg cgcgttcctt cacgacgcgg tccatcaccg cgcgaacccg ggcatcgagc
     2761 tcatccggtg cgacctggag tacatcgctg atggaccgcc acaaacccat cagcggcacg
     2821 ggcaacatga tgagagccat gctcagcaac gcaatggcat ccaggtacgg gatccatcct
     2881 tgccatcgcg tgcccgcgag cgcctgggcg aagatgaagc caatcagcag tgcgagactg
     2941 agtacggtac tcacggccca actccgcgca tccagctcga gcataggcga ctgaagcacc
     3001 ctcgcccgcc ggcgcaggac cattgtcatc gcaagaccga tggcgcagag aatgcccgcc
     3061 caccacgctg ccaggccgta ttccacttcg tgaccgccgc tgaacagcga attcactgca
     3121 tcgacggcgg cgtaaatgca ggccaccgcc agaatgctgt cacgcagcac gcccacgagg
     3181 ggttcgagat gccagtagcc aaactggaat cgttgactcg tcccccgggc gatcagtttc
     3241 accacgagaa gcgacacgat ggttggcaag acgtcgacga agctgaacat gccatcgaag
     3301 atgatcgcct gcgatccagt ggcgaggccg ctggccacgc cgagcacgcc cagcagaacc
     3361 gtcaacgcga tcgaaaactt gagcgtgcct tgttcggtaa tcgctgacat tcgacgagca
     3421 tcctctcacc tgagcccatg cttccgtagg accaacgtcc gattgagccc gacggcaggc
     3481 gttctcatag ttagcgcgga ggtgcctgtg aaccaaatgg gtcgattccg tctcgggacg
     3541 ctctcgtcag ccggcctgtg gctatgcgcc atcctcgctt cgatgcccgc aagcgccctg
     3601 ccgctgctcg acgagttgcc cgagctcagt accttgagca gccgcctcga actcactccg
     3661 gaacaggaaa acaagctcgt tcccctgttt caaaaccgcg catccgaatt gaggcaatcg
     3721 caggccctcc tcgaaaaagc caccactccg caacagcaaa aagaagtgct gcgtgacgta
     3781 gaacaggccg ggaaagaatt caatactcag gtcgagcggg tgctcacgcc ctcgcagcag
     3841 gatgaatggc gcgaattcat ttcggacctt cgcgaaaagg cgaaggaacg catcaaagac
     3901 aagcgcgaat cgcgctagtt agagaccggg atcgcgcgga tttgcgagct cgagcagcgc
     3961 agcgcgcagc aggttgagtc gcggcggctt gctgagcacg cgatcgatgt tttccggcag
     4021 ggtagcttca tccagcagtc gctggcccca gccggtcagc aggatgatgg gggtcgttgg
     4081 cgaagaatgc ttgatcgccg ccgaaacctt gcggccgtcg acccgcggca tgccgagatc
     4141 ggtgagtacc acctcgaact gctcttcagt gccacgcgct gccgcaaagg ccgcgatgcc
     4201 ctcctcgccg ccgagtgcgg ttgtcaccgc gtggccgtcg gcttccagca cctcgagcag
     4261 agagcggatc aatatcggat cgtcatcgat caccaggatg cgcagttttc tcaggctcac
     4321 atgcgaggca agttgcgagc gggccgccga tgcgtcctct gtgaatgcgg ggaagatcat
     4381 gcgcatcgtc gtgcccttgc ccgacgcact gtcgacttcc agcacggcgc tgtggcgctg
     4441 gagcatgccg tacaccgacg ccagtccaag tccggtaccg cgttcgccct tggtggtaaa
     4501 gaagggctcg atgcagcgac gctgggtgtc ctcatccatg ccgacgccgc tgtcgccgac
     4561 ttcgacacgc accgtcaccc gaccatcgcc atcgacgaac gggcgcgttc gcaaggtcag
     4621 cgtgcctccc tcgggcattg catcgacggc attgaagacc aggttggtca gcgcgtcgcg
     4681 aagctccacc tcgtcaccca tgacgttggg caacttcggg gcgaagtcct tgagcagctc
     4741 gatgacgatg ccgcgctgct gcggctgcgc gcgccagcgc ggctgcgtga gatcgagcac
     4801 ctggttgatg gcactgtgta tgtcgagctt ggtgaacgcg cgcgtgagct ctcgtggccg
     4861 gtagaactcg cgcatgcgat cgatggtgcc ggcgacatcg tcgatcgctc gccgcatcgt
     4921 gaccaggcgg ccacgaccgg cttcggtgat ctgtgtttcc tggctgagca gcaactccgc
     4981 gtatagcgcc acgggcgaaa tggcgttgtt gatgtcatgc gcgatgccgc tcgccatttc
     5041 gccaagtgca cgcagccgct cggtctgcat cacggtctgc tggctgtggc gcagatcttc
     5101 ataggcacgt tgcagtgcgc cgtaaagctg ggcttcgtgc gacgccagcg ccacgtgctc
     5161 gctgagttgc ttgaggaatt cacagtcgcc gctggaaaaa gcttccttct cgcggcgcac
     5221 ggcgaccagc acgccgaaaa cgttcgattc cacggcgagc ggcgaaatga ccagcgaatg
     5281 gaaacccgcg tccgcgaacc ggcgcgtgaa cggcgagtcc actccggcgg tattgggctc
     5341 gtggatcaag gtgccgttca cagcccgata caggccgttt ttacccaccg gcacggcgct
     5401 gcccagcgcc aggcccagcg acttgcaaga cgtttcactc gcggccccca cactttcgac
     5461 cacgaccatg ccggtcgcag cgttgcacag gcacacgcaa ccaaaatcga tgggcaggtg
     5521 ttcctcgagg ctgcgcaaca ccacctggaa aatgcttttc aagtcatgcc gcgaaccgat
     5581 gctgtgggtg atctgctgaa gcagtgcgag ccggttcacc tgggcactca tgcgcttttc
     5641 gttctgctcg gtctgcatga gcatgtgatt gaacgcgttg gtcagatcgt cgagctcgat
     5701 gatgccggcg aggcgcgcgc gtacgccgta gtcgtgccgg tcggagacca gtcgcgcggt
     5761 cgcggcaagc tcgagaatcg gcgtcgacag ctccttttgc agacgccgcg agatggccca
     5821 ggccgcgaac agggcaagcg cgatcgctat cgccgccacc agcgcgtaga acgcgagccg
     5881 atcgtaaacg gcgctgaagt cggaccggac gtacaaggtc ccgagctcgc gtgcgccctc
     5941 gcgcacgcgt gcgacaccga tgagcgccga acccgcaatg ttgtagcccg gtggccccgt
     6001 ggcctcgggc agtgcgccgg ctggtgcgag ctccggatac gcaacgaaga gctgcccgtc
     6061 gctcccgtac agcgcggccg caatgatgtg cggatcggac ttgaatgcgg cgagtaccgc
     6121 gcgcgcgtct tccggattgt cgaaggcgag cgcggcggtg ctgttcgtgg cgatggcctc
     6181 gctgaggatt cgcagctgct gcaggctcga gtcacgcgcc gcaacgattt ccccggccag
     6241 gaacactgcc gttgtcagca gcagcgccgc gccggtggac accagggaaa tggtgctgag
     6301 tttcctgcgg atgggcgccg cgcgcgtggt catctcaccc gccttctttc ccgacgacat
     6361 cggcgggtcg cagcagcttg gaactgatga ccaggttgtt agccttggcc gtcgccacgt
     6421 tgatgcgcat gcggattcga ttgttctcgt tgacgagggc gatgacggcg ccccggtgct
     6481 cggcgcccgc gatgtcggtg acggtgagca cgctgcgacc ctggagcacc ttgagcgttt
     6541 cctcgaggcg cgccgtttca ctgcggccga tgaaaagaat gttgcacgcc gagatgtcct
     6601 cgggattctt gaagcgccgc acgaccagcg ggtggccgcc cagcgattcg cccgcgatca
     6661 cgtcgtcgag aatctttccg aaagggtcat cacccacgac accgatcacg aaaggcgcgc
     6721 ccggcgaatc gtaggcctgc agcggccact cgacgaattg cccgaagttg aatagatagg
     6781 cggctttgac ctggtactct gcaaccggcg gcgcggacag ggcggcgctg cagacgcctg
     6841 ccgccacgac acacactgcg cccagcagcc agcggcgcaa tgccgtgcca cgagcagacg
     6901 ggcggagttg caacggtgcc atggctggtt agttgcgcca ggtcgccttg agataagccg
     6961 cgcgctcgag cgcgccacgc cggtcgattg agccaaattc cacgtgattg tcgtgcagca
     7021 ggttctgccc gacgagcgac aagctccagt tcgccgtggt ttgccaaccc aggcgcaggt
     7081 cgagctccgt gaaaccgtcc atgctttcgc cgctgaccac catgggctgc ctctcgatcc
     7141 gcgactgata acgcagctgc gcatcgatct cgacgctcgt cccgagagtg agcaacgacc
     7201 gcagtcccgc gatgtcgcgt ggcgtggcgc cttccagcca ttcgccgcga ttgaggtcct
     7261 gcccctcagc gtcgaggctc atgtcgatgt gcgaatagct ggcgctcagg cgccaggatt
     7321 cgacgggctg ccactccacc agcaattcgg cgccgtaagt ccggcccgag gtcaggtttt
     7381 cgttgatcac cggaatcacg gtgcggccgt ccgcagcgat gaacggttca ccaatctcca
     7441 gtgaggcaag gcggtcgtag tcgttgtaga agagcgcgag atcgaaggac aacctttcca
     7501 gcggctgcca ccggtaacct gtttcgtatg cgacgagaag ctcggaacgg aaatcttcat
     7561 tccccagcaa tctgcccacc gggttgccgg cagggtccgt gagatcgatg gcgatgtcgc
     7621 gttctatgcg cgtcggcacg cgcactgcgc gcgagatcgc cacccacagc gtgtggttgt
     7681 cgcgcggcag ccaggccagg cgcacgctgg gctgcaactc gaagccgctg aagtcgttgt
     7741 gttcgagttt cgtacccacg gtgacgcgca gttcccggcc aagcgatatc tgatcctgga
     7801 tgaagccgct gaacagctgg tcggtggact tctccggttc gaccgcgaaa accacgcgac
     7861 cctggttggt gttggcggtg tgccggtatg cggcacccca gaggatctcg tgactcgacc
     7921 cggcgttgaa acggcgctgc aggtcgatgt caaaggtatg cagcgtgtcc aggaagctcg
     7981 ggtcgtcgcg gcgcgtgtag tcatagtagg cacgcaactg catgtcggcg ccgtcctcgg
     8041 cgctgcgccg ccagcgcgcc aggatattgc cgccgctcaa gtccacgttg agcggcggcg
     8101 gcggccccgg gcgcccgatg atgtttatcg ctgcggccag ttgcccgacg tttgcccggt
     8161 aggcatcgcc ctgcacggtg aacgagtcgt ggccggagcc atcccagtct gtgcgaaaac
     8221 cgacatgacc cagattccag gaatcctcgg tgtcaagcgt tggggcaaag gtcgcgtcgc
     8281 gctcgaaata cttgccgtac acgcggaaat tcacaccgcc gctggtttca cccccataac
     8341 gcccttcgag ccaggcgcgg tcgaaagtgc cagcgccggc ctcgatgcgg gtgccgtggg
     8401 tatcgcgcgc gctgcgtgtc gtgatgttga tgacgccatt cacggcgttc gagccccaca
     8461 gcgccgcccc gggaccacgg atcacctcga tccgctcgat gtcctctagt agatagtcct
     8521 ggacggtcca gttcactccc gagaacagcg gtgtatagat gctgcgcgtg tcggacagca
     8581 ccagcagctt ttcggagttg atgctgctga aaccacgtgc gctgaccgcc cagctgcttg
     8641 cggtctgctc gcccacgtgc aggcccggta cgagacggag ggcgtcggga accgtggtgg
     8701 cgccggaccg gcggatgatc tccgggctga tcaccgcaac cgccgcggcg gcatcgcgca
     8761 gggtttcttc ggtgcgcgaa acggaggtga tttcgacggt catgagctcc tcgaagctca
     8821 tgcgtttgag cgcgtccaga tgaccggcgg aactgttgtt agcgagcgcg gatccgggga
     8881 tcagagcgcc gcagatcagc aaccatgccg gaccatccct tgacgtcacc ttcaatcctt
     8941 gttccggtgg acccgaagtc aaaatatcgc aaagcagcac acattagaca gcggggtttc
     9001 tcgtaaaaaa ctccgcaggc tgcggattgc tcaacttaag tcgaagtttc gagcgaacct
     9061 cgcgcgttcg aaggtgctgc gggcatgcga agccacgttg tcgtatcggc cgaaccttct
     9121 ccgatccacc tgagccagga aaagctggcg tttctcgtga atctgtcccg taactccgtc
     9181 attccgatcc tgcacgagtt agttcgactc ggcttcatcg aagtgaaata cggatcgatc
     9241 gcggtcgtcg acgccaaggg tttggcggcg accatttctg gacagaaaaa tggccggatg
     9301 agaaaaaggg tgagagcact cggctctcac cctcttgttt catcgacccc ggatgatcag
     9361 ggaatcgggc acgaacccgc gcaggcgctc gtattcatct ggctcggcgg cagcaaggtc
     9421 acgtctgcct ggctgacctg gcagttcggc gtgttgaact cgacgcccag cgccgaagtg
     9481 ctgccgttgc ctttcaccac ccctttatag acctggcaga tcttggtctt ggtggtgccg
     9541 ctgttgttca gcgtgagatt gcgaatcgtc gccacgtcac cgaagttgga attgatgcca
     9601 acgatcgtcg gaacgtcacg cgtgatcacg ttggtcaccc atatcttgcg aggacccccg
     9661 ttgcccgtgc agtcgccgca ggaacgcacc agcttgccgg cattggcagc gtagaaattg
     9721 ctgatgcgca gctcgcctcg gccgttgaac tggaacgtct tgtcagagcc gttgtaggcc
     9781 gcgccacagg tgatgttcat gacagtgccg gccggcccga gcgccgtcgc ggcatcctca
     9841 ccgatgtcct gccaccacac gtggtcgagc gtgcaactgc ccaggcaatg aatgccatcc
     9901 gcggcgagcg tgccgatgat cacgttcttc agcgtggcgc cgtgctgcac gtcgaacacc
     9961 gcgggctgac cttcactctg acttccaccg gaaaggttgt agaccttgtt ctggccatcg
    10021 aacaccgtgc cggcgggaac aacctgggtc gcggtcaact gcacggtctg ggtgttggtc
    10081 ggcattgcgc agcccgggta atagccgagg tccgcggtcc caccaccagg cggcggcggt
    10141 ggcggtggcg gcggaggtgg cgcggagccc gtgggcacgg aacactcgtt cacgccgcca
    10201 gcgattctgc cgccgaatgt ggccgaggag cacacgaagg agccgctcag tgtcttgaag
    10261 agaaactggt cggcgcggcc gaaggcgacg ttggtcgatg cggccaccga acaagtcttg
    10321 ttctcgttgc agatcgtcac gtagcccgcg ggcctgtttg cgccgagcgc cgcatccgcc
    10381 agagcgaatg ccgccaacgc atatagatag tgtccgtact tcatcgtgtt cccccttgtg
    10441 gttgattcct ttctacagaa ggcgtccacg cgcagggaaa agctggaagt gcccgcgtcc
    10501 ttggccaccg gtgtcatacc tgtagacacc ggtgtcatta tggacccggc gacgccgcga
    10561 tcacaatcgt cgggaagccg cactgcagcg agcgtgaatc ctgcaggcac cgtcccacat
    10621 agaaaaaagg gtgaaagcct cgcggctctc acccttcaaa tggcccccta acggcccctt
    10681 gcagatcgtt ctctatggaa tcgggcacga tcctgtgcag gcgctcgtgt tcatgacgct
    10741 cggcggcaac agcgtgacat ccgcctggct cacgttacac gccggcgtat tgaactcgac
    10801 gccgagagcc gtgctgctgc ccgaaccttt gacgacaccc ttgaagacct ggcagatctt
    10861 cgtcttgctg gtgccgctgt tggccagcgt caggccgcgg atcgtggcca catcaccgta
    10921 gttggagttg atgcccacaa cggtcgaggt cacatcgcgt gcgatcacgt tggtaaccca
    10981 gatcttgcgg ggaccaccat tgccggtgca gtcaccgcag gtgcgcacca gcttgccgac
    11041 attggcaacg tagaagttgc tgatgcgcag ctcgccgcgg ccgttgaact ggaacgtctt
    11101 gtcgttggcg ctatacgcgg caccgcagtt gacgttcatt accgtgcccg cggcacccag
    11161 ggccgtcgcg gcatcctcac cgacgtcctg ccaccacacg tggtccaacg tgcagctgcc
    11221 gagacagtga atgccgtcag cggcgagcgg tccgatgatc acgttcttga ctgtcgcgcc
    11281 atgttgtacg tcgaataccg ctggctggcc ttcgccatcg ccgagagcgg caaggttgta
    11341 gaccttgttc tggccatcga acaccgtgcc ggcaggaacg acctgtgtcg cagagagctg
    11401 cacgacttgt gtgttcgtgg gcatcgcaca tccggggtag taacccaggt ctgtaccacc
    11461 agtaggcggc ggcggcggag gtggcggcgg cggcgggggt gcagtgcccg ggccggtgga
    11521 acactcgttc acaccaccgg cgattttgcc gccgaacgtg gtcgacgagc aggcgaagct
    11581 gccgctcaat accttgtaga gaaactggtc ggcgcgaccc atggcgacag tggtgttcgc
    11641 tgccaccgta caggtctggc cttccttaca aatcgttgtc cagccgcttg ggcggttggc
    11701 ggccgacgcc gaaccggcca gcgcgatggc ccctagcgca actagaactt ggacattttt
    11761 catcgccctc tccctccgat gaacccgaga ccctttgttc ttttgtggtg ttgaccaccg
    11821 gtgtcatttg gacacgggtt tccctgaggg ctggagctcg aactctcgca gcagtagcgc
    11881 taccaggcga cgccgctctc ataatcatgt gtcaaatgcg ccgctccgga gcggtgcgga
    11941 cgcggatatg cacttttacg tatccgcttt gcctagctat tctgtaaagc cgccagagga
    12001 ccgagctcat gccaaagtta ctgctcttca ttgcctgcgc attgttaggc ctgcggtcgg
    12061 cgcaggccgc cgactaccct gccccggccg aaggcgactt cgtgctgcgc gatttccatt
    12121 tctcgaacgg cgagtctctg ccagaactga agattcacta tcggacgctg ggctcaccga
    12181 agaaagacgc caagggcatc gtacgcaacg ccgtgctcat cgggcacggc accggcggaa
    12241 gcggtgcgca attcatccga cccgagttcg ccggagagct gtttggcgcc ggacagccgc
    12301 tagacgccac tcgttatttc atcgtgctcg ccgacggcat cggtcacggc aagtccgcca
    12361 gacccagtga cggcctgcgt gcgaagtttc ctcagtatgg ctacaccgac atggtcgaag
    12421 cgcagtaccg cctgctcacc gaagggctca aggtcaatca cctgcgcctg gtgatgggca
    12481 cgtcgatggg cggcatgcac acctgggtat ggggcgagcg gcacccggag ttcatggacg
    12541 cgctgatgcc gctggccagc cttccggtgc agatctccgg gcgcaatcgc atctggcgca
    12601 agactctcat cgacgccatc cgcaacgatc cggcctggca aggcggcaac tacaccgcgc
    12661 agccgccatc gttgagcacc gccatatcac tcatcttctt catgggcagc aacccggttc
    12721 tgcgccagga acagatgccg accttcgcca gggcggacga agttctcgcc gaaagcgtgg
    12781 cgaatgcaat acgttcgcag gacgccaacg acatccttta ccaggtaagc gcctcgtggg
    12841 attacgatcc agcgcctgca ctcgagcgca tccgcgcacc tctgctcgcc atcaactcgg
    12901 ccgacgacct gatcaatcct ccggagctcg gaattctcga gcgggagatc aagcgcgtga
    12961 agcgtggtga ggccatcctg attccactga gtccacagac ccgtggccac ggcacgcata
    13021 ctttcgccgc gttgtggaaa caacacctgg tcaaactact ggatgaatcc gcgcaatagc
    13081 ggcaaggtca gcgatcgaac cgcaccgtca ggatcgtgaa gtcctggccg cgattgggcg
    13141 tccggacgcc gccattggac cagtggcgcg cggacagctc gacgaccgcc ggaaattgcg
    13201 acaactggta tcgcagaccg aactgcgaag aaaagttcca gtgcgtcgcg ctcaggacgt
    13261 ccgtctccgt gcgatacgac aatccgaatc cgaagattgc acgcagcggc ccacgctcga
    13321 acaacttgaa acggcgcgac atcgacgcgc cccagtacgg acgcgccacc agccgctcga
    13381 cgttctcgct gttgtcggtc gaggtctgct cgccgaaatg gaatacaccg aattcccagc
    13441 ggccgcgatc ccaggtcaag gtcaccggaa tcacctcgcc gccggtcgct tcgaatgaat
    13501 ggtatgtgcc gcccagtcca acctgaagct cacaatcgcc gcagaacgaa ggtcgactgc
    13561 ggtcggggcg aacgtaggga acgttcgtgc caccgaggtt cgagaacgct tcgaggttca
    13621 tcagctgcgt gtcgtccaat gcagcgtgtg cgacgcgcag cgcctcattg gtttccagca
    13681 gtgacaattc gagcgtgtgc cacggcgtcc tgtccgactc attcaaaccc gcgccaattc
    13741 cggctgcgga cccgaacatc agcacgcccg cgagcgccac aatgactcca gccctgttca
    13801 tatccgttcc gtttcttttc ctgttcattg aaacgaattc ttgacttagt tggatgcaaa
    13861 gaaaattgca ccaaggtgtc ggccggatac atccaaagaa aggggtccaa cctacctctt
    13921 tggagagaga tcgaagtcgg ctcacaaagt agattgttgt cagcggagtt gaagccggcg
    13981 aagccgccca caggaagttc agcatcatcc atgacccgca ccgactcgaa aaactcacgc
    14041 ctggaacgca tcgccgcatc gccgcgctgg gatggcgtcc gctttcgcaa ctcgagtccg
    14101 atttcgcgtg gcgatcccac ggttgccatg ccgtcgatga gcgaattcat atgtggaggg
    14161 gatcgacgag tgccgcaggc gccgctgcca tcccgcaatc ctctcgatat ctggacgcgt
    14221 cccgcgggca gcggcttgcg tgcgacgtgg ctcggccact cgaccgtgct gatcgagatc
    14281 gacggctggc gtgtgctcac cgatccggtc tggggtccgc gcgcgtcgcc ttcacgcttc
    14341 atcggcccga agcgtttcca gccagttccg gtcgcgcttc gcgagttgcc gcctctcgat
    14401 gcggtcatcg tttcgcacga ccactacgat cacctcgact acacgaccat gcggctgatg
    14461 cgcaaagcct cggtgccaat catcacctcg ctgggtgtag gtgctcacct cgagtccttc
    14521 ggaatcgcgc cggaacgcat cgtcgagctc gactggtggg aatcgcatcg agtgcccggc
    14581 accgatctca ccctgcatgc cgggccgtcg cagcacttct cggggcgtgg tctcaaagac
    14641 ggcaacaaga ccctttggtc gtcgttcgtc attcagtcgc agcgccatcg cgtgttcttc
    14701 agcggagata ccggcccgac ggccgaacac gccgcgatcc gcgaccggct cgggcccttc
    14761 gacctcgtca tgctcgaagt gggcgcatgg caccccgcgt ggggcaacat tcacctcggg
    14821 ccggccaacg cgctcgcggc gcataccttg ttaggtggtg ggccgcttct gccagtgcac
    14881 tggggcacgt tcaaccttgc gttgcacccc tgggacgaac cggtcgaaac actgctgcag
    14941 ctggcaccga aaacctctgc ccggctcatc ctgccgcggc tgggtgagcc cgtggagccg
    15001 gcgcatgatt tttcggtgac gccgtggtgg agaggtatcg acctggacgc gcgcaatcca
    15061 cggcgcggca gcacgcgcat gccgaaagcc atgccctttc cacctgacta gcgcggccgg
    15121 tcagggcaga tcgaatacca ggacttccga cctggaccct tgagcgattt ccaggacggc
    15181 ttcttcggtg agtttgagtg catcgcccgc gtcgagctcg atgccattgg cgtgcactga
    15241 acctcgcgca acatgaacat acactcgccg tcccgctgcc agcgggaggc tggcgtgttc
    15301 cgcgccgtcg aagagaccgc tgtagacacg cacatcctgg tggacgagca ccgagccggc
    15361 ctcaccattc gacgacgcaa tcaatcgcaa ccggccttgt ttgtcggcgg cctcgaagcg
    15421 cttctcttcg tagctgggcg gtatgtcggc gacattcggc tggatccaga tctgcaggaa
    15481 atgcagcttg ttcgtctgcg agccgttgaa ctcgctatgc cgcacgccgc tgcccgcgct
    15541 catgcgctgc acgtcgcccg ggcgaatggt cgaccgcgtg ccgatggagt cctggtgcgt
    15601 gagctcccct tcgagcacgt agctgatgat ctccatgttg cgatgcccat gggttccgaa
    15661 gccctgcccg ccggtaacgc gatcttcgtt gagaacgcgc agcggcccga actccacgtg
    15721 atcttcgtcg aaataatccg cgaaggagaa gctgtggtag gacttcagcc aaccgtgatc
    15781 ggcatggcct cgatctgaac tgcgtcggac gactttcatg ggactagact gtacccattc
    15841 agcaaggggc gcacatgaaa gagaatcgag tcggcttcat tggcctcggg cgcatgggcg
    15901 ccaacatggt gcggcggctc tcggccgccg gatttcagtg catcactttc gacgcgaacg
    15961 cggcggcggt gcaggcactg ctggcgcccg gcatcacggg cgcggcgaca ttgcaggact
    16021 tcgtggcaaa actcgcggca ccacgggtca tctggctcat gctgcccgcc gcggtcgtcg
    16081 accaggaact caagagtctg cagcccatgc tgacggcggg agacaccgtg atcgatggcg
    16141 gcaattccta ctatcgcgac gacctgcgcc gcggcgccga actggcgaag gcgggcattc
    16201 actatgtcga cgtcggtacc agtggtggca tcgccggcgg cgagaacggt tattgcctga
    16261 tgatcggtgg cgaaaaagcc gcggtcgatt cactcgagcc gctgttcgcc gcgctttcgc
    16321 ccggcgggca gagcagctac aacaattccg acaaacaaaa tacggcgcaa cgcggttacc
    16381 tgtactgcgg accctacggc gcgggccatt tcgtcaagat gattcacaac ggaatcgagt
    16441 acggcgtgat ggccgcgtac gcggagggtt tcaacatcct gcgcaatgcc gacatcggca
    16501 cccggccgcg agagaaagac gcagagacca caccgctgca acatcccgag cactatgcat
    16561 acgaattcga cgtcgcgcag attgccgaag tctggcgcca cggcagcgtc gtccggtcat
    16621 ggctcctcga cctgaccgcg cgcgcgttgt ccgaagacgc gagcctggcc gcgtttgccg
    16681 gccgtgtctc ggattcgggt gaaggacgct ggacggtggc ggctgcggtg gacgaaggtg
    16741 tgcccgcgcc ggtcataacc gccgcgctct acagccgctt cgagtcacgc ggcaatgccg
    16801 actacggcga ccggctgctg tccgccatgc gcaaacagtt tggcgggcac gtcgagaaga
    16861 aaggcggcaa ttgaccccgc gcctcagccg tattcgcgca ccatcgccga catttcgcgc
    16921 aggaaatcgc cggagcgcac gccgctgccg cccgagacga acggctcccg gtaccagggc
    16981 cagggataaa cgacgctgcg ttgcggccgc ttgccacgca gcagcgtgaa ctcgcgcgta
    17041 ctgaagccgc ggcggatgct ggtgtcgatc tgctccggag tcaccgcgta cactgcgcca
    17101 tccaccagca cgaacagccc ggccttatag ttaggttgcg cgtcgaagcg ttcgcgcgac
    17161 gaagcgaagt atgcggcgaa caccgggccg ttcaccgacg cgatctccga gccgacgccg
    17221 agcacgtgcg ccgggttgcg cggttcgccc atgggctcga cttctgcgcg ggcagcattg
    17281 atgaacaggc tgtagaacgc ccttgagcct gacgagtcgg gttgaaagcc catgacgcag
    17341 actcgtccgg agtccgggag tctaccggtg tcatttacga acattggggg tgagtctccc
    17401 gatcccccga aaaggttctc tgcatttgcg gaattattgt cccggcggtg gcagggagac
    17461 aataacccgc aggccttctt cttgcgattt cgcagtccca cctgtccgca gtggccggga
    17521 atcatgtccc gaagggttgt caggggcgga ctaaagtcgt aggcgagcac gctcaccatg
    17581 tttgagcgct ttgtccacca ggctgaccac gaggctacgc ggggacagcg gatcggccgg
    17641 cggatgaacc cgtccccacc ctcccgaatc cgaagacatc gtcgagcgca tgcacgcggc
    17701 cttcgacccc ggcgcgaatg atgtcgcccg cgtgcacgct gtaggtgatc ccgttgccgc
    17761 cataacacag cgcgaactgc agccgcggac tcatgccggg cacgcgcccg atgagcggca
    17821 ggccatcgtg agtctcggca aaactgccgg cccagccgta ggcaacgggc ggcagctccg
    17881 agccaaacaa cgtctggtac tgctgctgca accggcgaac ctgccgcggc agcattagat
    17941 cgcgggcagc agcattcttg aacggaacat ccgcgccgcc catcagcagg cgtccgtcgg
    18001 aggttccacg catatagaag tatggccggg cgctctccca gatcagtggc aacgtcgcca
    18061 tccgctcgca cccggtcaat ggctcggtga ccagtgcaaa ggtgttgtga acgtcggcgt
    18121 acccggacgg caggaagtcg agggactcat atcctgcgca aaccgccacg tgagcggcag
    18181 tcacggagtg accggccgcg gtgcgcagta caaatgcatc gccctgctct tcgatctgcg
    18241 tgactctggt ccgcgcgaag agctgaacac cgtggcgccg gcaagcggaa agaatcgcgc
    18301 gcgtcaaacg gaacggatca acctgggcgc cgagggccga gagaatccct ccgggccgaa
    18361 ggcaaccgaa ccgtcgccgc agctcatcgg cgtctaacca ctcgcacgcg aggccgatat
    18421 ctcggcgcgc cttgagttcg acgagcagcg gatcaacagc cttctcatcg gcggcgagat
    18481 acaggctggg gcggcgctcg taacccgcct cgggcagcag ttcggggaac cgtgcttcga
    18541 gctcggtgaa actcgcggcg caggcacggt aggcacgcat ggcgcgctcg gcgcccaaca
    18601 gcggcacgag atcggtgaga tgcgtgtcga tttcgtactg cagcagcgcc gttgatgccg
    18661 aagtgcttcc ctgcgccacg tcgcgggagt cgagaacgac gatgcgcttg cccgtggcga
    18721 tcaacgcgtc ggccgtcagc gccgcagtga tgcccgcgcc aatgatggcg atatcacaat
    18781 cgatggattc ggtgagcgcc gggctggcat ccccgatgcc attgcgaagc agccagtacg
    18841 cctcgctcga ccggatcgag cccgcggaat tcttgctgaa tggccacatt cagcgagatt
    18901 aggtgccgct gccgccgggc cctgtaagaa aaagcccgga ttggcccgct gtcatgcggt
    18961 ccaatccggg cgtgcgatca tcgccgcttt tcgaagcagc tgtcctttac ggcgccgccg
    19021 ggctgatcac gacttccaca cggcggttct gttgccggcc tgcggccgat tcattgtcag
    19081 caaccgggga attctctccg cgtccggaag agctgagccg tgcggagttc acgccccgcc
    19141 cgaccagata ccccttcacg gcatccgcgc gacgctgtga caacgactgg ttcatgtctt
    19201 cgctgcccat gctgtccgta taaccttcga tggctgccgt gcgctgcggg tactcattca
    19261 tgaacgcggt caagcggtcg agattcaccg ttgcaccgga cttgatctcc gacttgccgg
    19321 ttgcaaacag ggtgtcaccc aacgtcagca cgagaccgcg atccgtcggt cgcgcctgca
    19381 ggatttcaag ctgtcgctgc agctcgagtg cctgctgttg cgccgcatct gcactgtcgc
    19441 gcgcctggcc cgcgatcatc ttctgctcgg cgttttccgc acgtgcgacc agcgcgtcgt
    19501 tcttggcgac atccgcttca cgcgtgcgcg aatccaggcg cgccttctcg ccctgctcgg
    19561 tgagcgccgt ccgctgctgt tcggcgacgt gcgtcacggc cagcgcgcgg gcagtttcga
    19621 ccttgcggtc ggcgatgtac acgcgatgcg cagccagcgc cagatccttc tcgatcatct
    19681 cggccaacac cacggcgccc tcggcgtcct tcatggccac cggcgctcca ttggcgagcg
    19741 aagtgtcgcc ttgcagtgcg gtgagcttgg cgcgcacctc cgcggaaccg gccggcctct
    19801 gcgtgctggc acaactggcc agcaggacgg cggcaacggc cgcaccgatc agggtgctgg
    19861 aaaatgagta gttagtgatt ttcatcggct ttctccggtc ttgcgctgca tctcgtcgat
    19921 gagcgtcgcg gtgctgcgct cgatgtcctt gttcacggcg accgccttgg cggcgccgga
    19981 tttggccgcg gccagttcgg ctccggcgcg cgcctcatcg gcgaactgtt gcgcgacgac
    20041 catttccttt tcctcgaccg cgcgctgcgc ggcggcaagc ttgccacgcg cttcgccgag
    20101 ttcgacaccg gccagggttg ccgcatccac gcgttccgca ttggcaatgg cctgctgggc
    20161 ggcctgaagg ttggttgtgg gcacgggcgg acgcgcgaaa gcggtgctcg ccacgagcac
    20221 gaggaccggt agcgcgatgc gcaggcggcg tcgcgaaacg ctcgttctct tggaagggga
    20281 atacatagag ttctctactg atgacgacgg aggacgcctg ggcggctctc caccagagaa
    20341 cgcagagtgg acttcgcgtt cgcacctgtc ggtgcgaaag cgcacatacg cattttgtcg
    20401 gcggtcgcct acgtgccggg agcgccgggt tcaagggtca aggcagcccc cggtagatag
    20461 gttcgcgctt tccgttgcgc gccgccacgc tgagccctgc accgatgctg acgcttctgc
    20521 gcagcacctt ctgcagctcg atggccgtca cgggtttgac gaggtaacgc tcgaatccgg
    20581 ccgcgcgcgc accttctcgt ccggggtgtt cgatattgtc cgtcaacgca atgagtcgga
    20641 ccgcctgcag gtctgcatgt tgatgcagga gcttcgccac gtcgtagccg ctcatgtcgg
    20701 gcagctctat gtcgaggaac acgatggccg gcgcatgttc cacggccacg gcgacggcgt
    20761 cggccgcgca atgaacgatg cgcgtctcgt aggcacccac cgtttcgagc aaggcagcga
    20821 gagatttcgc gatctcgacg tcatcatcga ctatgaggat gcgcggaggc gcgtcgctca
    20881 tctcgtgacc ggcaccagcg tcaacgcggt cggttgcgcc cacaacgagg gcagcagcac
    20941 ggtgaactcg ctcccaaggc ccaatcccgc actcgccgcg tgcacgctgc cgccatgcat
    21001 ttcgaccaca tcgcgcacca cggcgagccc gatgccactg ccgccttcgg cacagggcgc
    21061 cgaggcgtcc gcctgggcaa acatgtcgaa cacacgcagc agcacggctg gctcgatgcc
    21121 aatgccggag tcgcgaatac agatgctgat ggactggccg agagactcca tcgtcaacat
    21181 gatctcgccg ccatcgggcg tgtacttggc cgcattgatc agcagattcg agaacacctg
    21241 ttcgaggcgc gctgcgtcga cctccgccca gagcggttcg gcaggcagat gcagtacgaa
    21301 atgatgaccg cggcgtgcca gatccggtgc gatggcggca acggcaaact ccgcgatggc
    21361 gcgcaggtcg acgtgcgttc ggcacagcgc tctcttgtgg acgtcgcgat gcgaggcgtc
    21421 gagcaggtcc tcgatgtggc gggtcatctg tcctacgtgc cgctcgatga gaatgcgcgc
    21481 gccatctacg ccgctgaccc ctacggggtg gcgcaggagt ctggcggcgt tgcgcaccac
    21541 ggccagagaa ttgcgcaatt catgtgaaat gaccgcgatc tcgtcggcac gagcgtcggg
    21601 cagatgcagc ggcgccacga ttcccggcgt gctcggcggg tcctgacggt gcaacagcgt
    21661 ggccacactt ccgagcatcg atttgatcag taaaggcatt taaacctccc ggtcggcgtg
    21721 attttcacgg ccggccgcca aaaggcagtc gacgtcttgc cgccagcgca atgacagaaa
    21781 gcagcagcag gattgccccc aacgccaccc agggccaggc gttcacagtg catccccata
    21841 ggttctccat cgcataagcg gaaacctacg cgcccgtagt catcgggtct gtacgttatc
    21901 gaacacatcg cccgatatcc gccgagttat gagcggtagc gtacaaagtc ccgcgaatgc
    21961 gcctagactc ctaacaaatg aacctcaagc cggaaccgcg acagaaccac atcctcgatg
    22021 cattgccgcc gttggaacgc gaacggctgt ttccgcacct caagctcgta cccatgccgc
    22081 tcggcatggt tctgtacgaa tccggcgccg cgctgcgcca catctacttc ccaacgaact
    22141 caatcgtctc gctgctctac gtgatgcagg atggagcatc ggcagaaatc gcagtggtgg
    22201 gcaacgaagg cgcgatcggc gtttccctgt tcatgggcgg cgaaaccacg cccagccgcg
    22261 caatcgtgca gagcgcgggg cacgcgtacc ggctcaccgg caagcgcctc aagcaggaat
    22321 tcaatcgtca cggcgagatg ctccacatcc tgctgcgcta cacgcagtca ttgatcacgc
    22381 agatggcgca gacggccgta tgcaaccggc atcattcact cgaccagcag ctgtgccgct
    22441 ggctgttgtt gtcgctcgat cgcctgtctg gcgacacgct gcagatgaca caggaattga
    22501 tcgccaacat gctgggtgtg cgccgcgaag gcgtcaccga ggccgcgggc aagctgcaaa
    22561 agctcggcgt tatcaaatat gtccgcggca agatcaccgt gctcgatcgc ccgcagctcg
    22621 agagtttgtc ctgcgagtgt tacgcagtgg tcaagcggga atcggatcgc ctgctggcgc
    22681 cgcatccgct gagtcattcg gctgaggcat aggcgcttcg cagggcagcc gcacggtgaa
    22741 ttcactgcct tggtcggcgc cagcgaccac cgccgaaagg gtgccggcgt gcgcttcgac
    22801 caggtatcgc gccagataga gacccagccc gaggcctggt tccaggtcga agatcatgtc
    22861 gatttccgct gcagcaactc cgagcttgag gttgctcacg acaatgacag cctgaccacc
    22921 ctcgcgccgc gcgtgaatgt gtgtggggtc atgcgtaccg gcgaacttgc tggcgttgcc
    22981 gatgatgttg tgcagcgcct gcgcgatacg caccacgtcg acgttgaggt agacaggatc
    23041 cggcgagacg ctgaccgaca ggccatgccc gcgctcgctc gcgaccaggg caccggatgc
    23101 caacacgcgc tccaagagct cggataacag cgcgcgtgcc agctgcaact cgaccgcatc
    23161 cgtgcgcatg cgtgcgaaat cgatgagatc tgctatcagc cggttcatgc cactcacctg
    23221 gtcttcgatg atctgcgcgg cgagcgcgat cgtggcggca tccaacgact cttcccgcag
    23281 cagcgccgcg gcattgcgaa tcggcacgag cgggtgacga agctcgttcg cgactatggc
    23341 gacgaattgc aggtgagtgc cgtcgggcac gacagcggtg ttcatgagtg catttaccgg
    23401 tttaccgaga cagggtttgc cggatggtgc aacgagccat gccgccattc cgtacgctgc
    23461 cgcacattgg agatgcccac ccggatgatc gcattcacaa gggtatcggg cgatttcagg
    23521 acgtccgttc gctggcgaac agagcccggg gcgcagcgcg tggatgctgt ctgcgccgga
    23581 ccccccggcg cgagcaatca tggaaccttc gtgccgatca aacgcgcccc gtccgaaaac
    23641 ctgctgctcg ccgcaatgtc gaacgaggat cggctgcgac ttctcggcga ctccgtggca
    23701 atcgatctgc gactcgacga aacgctgagt cattcggacg atcgcattca ccacgtctat
    23761 tttccgatcg acggcttcat ctcgctgatc acaccgggaa tcgggcgcgc tcagctcgag
    23821 atcggcctgg tgggaaacga aggcatgttg ggtgtgccgc tgctactcgg catcaatgtg
    23881 tcgccactgc gtgcattggt acaaggcagc ggtcactcgt ggcgagtgga cgccgcccac
    23941 ttcctgcaag tcatcgagag ccatgcgtcg atccggaccg ccctcaatcg ctatctatat
    24001 gtgttcctcg cgcagctcat gcagacggcc acctgcacac gctttcacct ggtcgaagcc
    24061 cggctggcgc gctggctgct gatgacacgc gatcgcgccc attccagcca gttccacatc
    24121 acgcacgaat tcctggccta catgctcggt gtgcggcgag tgggcgtcac cagtgccgcg
    24181 agcgctctgc agcggcgcaa gctcatccgc tacaaacgtg gcgacatcgc ggttctggat
    24241 gttcgcggac tggaactcgc cgcctgcccc tgctacgcca tcgacaagaa gatctacgga
    24301 cgcgtgctcg gcaaagggcg cggatcacgg ctgcacgcgg gccgcgatcc ggcggcgatc
    24361 cgtctcgccg ctcggatcgc tggcgccatc tcctgctaaa ctggccacat caccgcgtag
    24421 gttgagtccc acagtgcgat gagtcatgaa ggcatggcgc acgggactcc aaattcgcgt
    24481 aatgctccga ggcgacgacc cagaaggccg gcggattctt gcacccattt ctcagcgaaa
    24541 tcatcgagca cttgtcgcct ggaacccact ggagcgacga tcagatcctg cgcgaagaga
    24601 tcttcagcat cgaacggctg gaggagcacg cgcgcagtct cgcggctgcc cagccggtag
    24661 gctcgatccc cggatttggc cgctcactgt ccacgcgcct ccgcagcaat gaaaaagtac
    24721 tgctcgccgc atatcgctcc atcgccacgg cggtggatga aggccgcagc attacccccg
    24781 ccgccgaatg ggtgctcgac aactaccacg tggtcgaaga gcagatccgc gaaatccgcg
    24841 aggatctgcc gccgcagttc tatcgccagc tcccgaagct gacggccggg cccttcgcgg
    24901 ggtttccgag ggtattcggt attgcctggg cattcgttgc ccacaccgac agccgcttcg
    24961 agcccgagac gctgcggcgt ttcgtacggg cctatcaaag tgtcgagccg ctgaccatcg
    25021 gtgaactgtg ggcggtggcc atcacgttgc gcatagtgct ggtggagaat cttcggcgtg
    25081 ttgcccagcg catcgtgacc agccgcctcg cacgacagga agcggacgcg gtcgccgacc
    25141 ggctgctcgg cgtcaacggc cagcgcgtcg atccgaacgc gctgcgtacc tggcgcgcca
    25201 ggtcgccgac gctcaaatcc gcattcgccg tacagctggt gcatcgcctg cgcgaccagg
    25261 atccgaagat cactcccgcg cttctctggc tcgaagagga actcgcggag cacgagacga
    25321 gttccgaacg gctgcttcag gaagaacacc tgcgccaggg cgcttcgaat gtgacggtgc
    25381 gcaacatcat caccagcatg cgcctcatgt ccgatgtcga ctgggcggag ttcttcgagc
    25441 acgtcagcct ggtcgacgat gtgctgggca cctcgcccga tttcgcggcc atggatttcg
    25501 ccacgcgcaa cctgtaccgc aatgccatcg aagagatggg acgtggatcg aagctctcgg
    25561 agctggaaat tgccaaacgg gtcctggcga cggcggcggc cgccaccagc ccgcgcgagc
    25621 gcgatcccgg tttccacctg atcgcggaag gccgccggaa gctggagcga gaaatcgatt
    25681 tccacgcctc ctggcgcgac tggccgagcc gcatcgcctt caccgtgggc gcgcgcgact
    25741 acgtcgcggc catcgcgttc acctcggccc tggtgctgtc cattcccctg tcgctgctgc
    25801 aacaggctgg cattcacggc ggcatgcttt ggctgttagg cgtgctcggc gtcgtgccgg
    25861 ccgtcgacat cgcggtggcg ctcatcaacc gcgcggtcac gcgcggtgtc ggcgcgagcc
    25921 ggctaccggg cctcgcgctg cgagacggag tgccggccga actgcgcacg ctggtggcca
    25981 tgcccgtcat gctgagctcg cgcgcctcgg tcgaagagca cctgcatcgc ctggagatcc
    26041 actacctcgc ctcccccgac gaccagctgc atttcgcact gctgtcggac tggtcggatg
    26101 ccgatacgga acaaaaagcc accgacgagt ccttgctcga catcgccgta ctcggcgtgg
    26161 cgcgcctcaa tcgccgctat ggcccggccg ccgggggcga gcgtttcctg ctgctgcatc
    26221 ggcgccgact gtggtgcgaa acggaacagc ggtggatggg ctgggagcgc aagcgcggca
    26281 agctgcagga actcaatcgc ctgctgcgcg gcgcgaccga caccacgtat gtgacgagca
    26341 ccggcgcctc gccgtgggtg cccgacggcg tgcgctacgt gctcacgctc gatggggaca
    26401 cgcggctgcc tcgcgaggcg ccgcggcgcc tcatcggcaa gatggcgcac ccgctcaatc
    26461 gcccgcgctt tgacgccagg atgggccgcg tggtcgaggg ttacgccatc ctgcagccac
    26521 gagtcgcctt ttcgctgccg acgagctcgg aggccacgcc gtttcaacgc gcgttctccg
    26581 gcgccgcggg cgtcgatcca tacgctgccg cggtttccga tgtctaccag gatttgttag
    26641 gcgagggttc gttcgccggc aagggcatct acgacgtcga cgctttcgaa gcggcgctcg
    26701 caggacgtgt gccggaaagc tcgatgctca gtcacgacct gttcgaaggc atctttgcgc
    26761 gcgccggcct cgcctccgac gtcgaagtgg tcgaggattt tccctcgcgc tacgacgttg
    26821 cttcgctgcg ccagcaccgc tgggtgcgcg gcgactggca gctccttccc tggattctcg
    26881 ggcgccgcga cgtcggcgcc gccgaacacg ggcgcggcca cagccgcctg ccgctcatcg
    26941 gtctgtggaa aatgctcgac aacctgcgcc gctcgctgtc tgcgcccacc gcggtggcgg
    27001 cactggtggc gggatggaca ctgccggcga acatcgccat tccgtggagc ggtttcatcc
    27061 tgctgtcgct ggcgctgccg acgctgctgc cactgttcgc ggccatcctg ccgcggcgct
    27121 cgacggttac gctgcgcagc cacctgcgag cgttacgcct cgacttcggc atcgccctcg
    27181 cccagaccgg gttgctcatc agcatgctgg cctaccaggc ctggctcatg tgcgacgcca
    27241 tcgtgcgcgc gttgtggcgc atgtgtgtga gccaccggca catgctcgag tggacaccgg
    27301 ccgacatgct gtcgagcgcg cgctccgact tgcccagttt ctaccggcgc atggcgcaca
    27361 gcgtgatcct cacggtgctg atcgccgcgg ggctcgcggc tgtctctgcc ggctcattgc
    27421 ccgtcatcgc catgccgttc ctgctcctgt ggttcacggc gccgctgctc gcatggcgcg
    27481 tcagccgcac ccctccggcg gtcgcaaagt ctgcactgtc gactgtccag cagcgcgacc
    27541 tgcgcctgat cgcgcggcgc acctggcgct tcttcgagac gttcgtgacg gccgaagaca
    27601 atcacttgcc gcccgacaac ttccaggagg atccacgcgg cgtggttgcg caccgcacct
    27661 ccccgaccaa catcggcctc tatctgctct cgacggttgc ggcgcgcgac ttcgggtggt
    27721 gcgggctgcg cgatgcgctc gatcgcatcg aggccacgct gggcaccatg gtgcgcatgc
    27781 acaagtgccg cgggcatctc tacaactggt acgacacgcg cgacctgcgg ccgctcgatc
    27841 cgcgttacgt ctcctcggtg gactcgggca acctcgcggc gcacctcatc acgctcgccg
    27901 gtgcatttcg tgaatggcaa aaagtgcctg cgccgccggc gtccgccgtc gacggcctcg
    27961 ccgacgcgct cgacctggcg cgcgaggcgc tgcgcacgtt ccagttcgcg ccgggcctga
    28021 cgatcacgcg cggcctgctg gaaaccgcgt tttccgattt cgaggcgagc ctgcggccgc
    28081 gaaccatgcc gctcgatccg cagaccgacg cgctgcgcga tgccgccgaa cgcgccggta
    28141 cgctggtcga catggtgcgc acgctggcga gcgagagcga cccggaacgc agcgccgagc
    28201 tcgtctactg ggtcgaggcc gcacgccgca ccatcgacag ctggcgcagc gacctgctgg
    28261 cgcgcgatcc ggccgggttc atcgtcgagc acctcgagtc gctcgccaac atcgcactgc
    28321 agcttgcatc ggccatggag ttcgacttcc tgctcgactc gcagcgcaag ctgctgtcga
    28381 tcggctatcg cgccaccgac ggcacgctcg atgacagttg ttatgacctg ctggcctccg
    28441 aggcccggct tgcaagcttc attgccatcg ccaaaggcga cattccggcg cgccactggt
    28501 tcaggctcgg ccgtaccgtc accccgatcg gtgcgggcgc cgcgctcgtg tcctggtccg
    28561 ggtcgatgtt cgagtacctc atgcccgacc tggtgatgcg cgccccggga ggtagtttgc
    28621 tggcgcagac cagtcggctc atcgtgcagc gccagatcgc ctacggcgaa gaactcggcc
    28681 tgccctgggg cgtttccgag tccgcgtaca acgcgcgcga tctcgagctc acgtatcaat
    28741 attcgaactt cggcgtgccg ggcctgggac tcaagcgcgg actctccgag aacaaggtga
    28801 cggctccgta cgccaccggg ctcgccgcca tgatcgacgg cgaagccgcg ttggccaact
    28861 acaccgcgct ggccgagatc ggcgcacgcg gtcccttcgg tttctacgag gcagtggact
    28921 tcacgcccag ccgcgttccg gagggcgaga cgcaggtgat ggtgcgtgcc tacatggcgc
    28981 atcaccaggg catgagcatc gtcgcgatcg ccaatgccct gctcgacggt cgcatgcgcg
    29041 aacgatttca ctcggatgtg tcggtgcagg ccacggaact gctgctgcag gaacgcacac
    29101 cgcgcgatgt ctcggtagcc catccgcgtg ccgaggaagt gggcaccgcg gcgcgcttcg
    29161 cggatccgca ggtgccgcag gtgcggcgca tccgcagccc gcacgactcg gcgccgcaga
    29221 cgcacctgct gtcgaacggc cgttactcgg tcatggtcac cgccgccggc tccggctaca
    29281 gccgctggaa cgacctcgcc atcacgcggt ggcgcgaaga cgtgacgcgt gacgacacgg
    29341 gcagctatct attgatgcgc gacgtggaaa gcggccgtgt atggtccgcg ggataccagc
    29401 cctgcgccgg gcaacccggc agctatgaag tcagtttcac cgaggaccgt gccgagatca
    29461 ttcgcgccga cggcgacgtc atcagcaccc tcgaggtgca ggtatcaccc gaagacgatg
    29521 ccgaagtgcg cctgctgtct ttgacgaacg ggtcgatgcg cgagcgcgaa atcgaagtca
    29581 cctcgtatgc cgagctggtg ctcggctccc aggcctccga cctggcgcac ccggcatttt
    29641 cgaaaatgtt cgtgcgcacg gaattcctcg cgcgccagaa cctgttgctg gccaatcgcc
    29701 ggcgacgtgc gcccgatgaa ccggaagtct gggcctcgca tcacgccgtg gtcgaaggtg
    29761 tcgtcgtggg cgcaccccag cacgagaccg accgatcgaa atttctcggc cgcgggcgtg
    29821 agttgcgcgc accgctcgcc ctgctcgaag ggcgggcgct gtcgggaacc gtgggtacgg
    29881 ttctcgacgc cgtgttcgcg ctgcgatatc gcctgcgcat acccgcaggc ggcacggccc
    29941 gcatcgcgtt ctggacctgc gtagccggtg accgcgcgca attgctcgaa atcgccgaca
    30001 agcatcgcga tcccaatgcg aggacgcgca ccgccacgct cgcctggacc cagggacagg
    30061 tacagctgcg ccacttcggc atcgacgctt cgcaggccaa tgtgttccag caactcgccg
    30121 gccacgtcct gttcgccagc tcggcggccc gtgcttcggc cgacaagatc cagcgcggcg
    30181 cggccgggcc cgccgggctg tgggcacagt ccatctccgg cgatctgccc atcgtgctgc
    30241 tgcgggtcga tgacatggac gacctcgtga cggtccgcca gctcctgcag gcccacgaat
    30301 actggggaat caagcgcctg gccgtcgacc tggtcatcct caacgaacgc ggcgcgtcgt
    30361 atgtccagga tctgcaggtg gccatcgagg cggcggtgcg caccagcctc gcgcgcccgc
    30421 gcatcgctgg cgcggacacg cgtggcaagg tgttcgtgct gcgcaccgac ctcatcacac
    30481 cggaaacgcg cgggctgctg cgggccgtgg cacgcgtcga gttgtccggc aaacgcggca
    30541 gcctcgccga ccaggtggag cgcatgcagc cggtaccggc caccgcaccg cgacttccac
    30601 gacgcgcgct ggtgagcagt gtcacggtgg ccgatccgga agacacgcgc cggctggaat
    30661 tcttcaacgg catcggcggc ttcgcagctc agggccgcga atatcgcatc tcggcgccgg
    30721 atggacagaa cacaccgcag ccgtggatca acgtcgtggc gaatcgcaat ttcggtttcc
    30781 aggtggcgag tgacggtggc gggtacacct ggtcgcgcaa cagtcgcgag aacgcgctga
    30841 cgccctggtc gaacgatccg gtcaccaacc ggccgggcga ggctatctac ctgcgcgacg
    30901 aggaaagcgg cgaactgtgg acgccggcgc ccgcgccgat acgtcacgag aacgcccaat
    30961 actcctgcgc gcacggcatg ggctacagcc gattcgaaca cgtctcccat ggcatcgagc
    31021 tcgagctcac gatgctcgtt ccgctcgagg atcccatcaa gatctgccgg cttcgcgtgc
    31081 gcaacgactc tccccgccgc cgcagcatct cgatcaccgc ctatgtcgag tgggtgctgg
    31141 ggccgtcgcg caccgcaggt gccccgcatg tattcaccga gcaggatgcc gtctccggcg
    31201 cgctgctcgc tcgcaatccc tggaacaccg aatttccggg cgtggcgttc gccgacctgc
    31261 gcggcgcgca gacggaattc acctgcgacc gcagcgagtt tctcgggcgc cacggcacgc
    31321 tcgaagcgcc ggctgcgctg gtgtcgggcg cgcagctggc gggtcgcgcc ggtgccggac
    31381 tcgatccctg cgcggcgctg cgaacgcggc tcgagctcga gccgtcggcg gccaccgaaa
    31441 tcgtgttcct gctcggcgag gccgtgggca ccgacgaagc gcgcgcgttg atcgccactt
    31501 atcgcggcgc cgacatcgag aagctgctgg gcaaggtgcg cgaacactgg gacgaagtca
    31561 ccggccacgt acaggtgaag acgcccgacc gcgcgttcga catcatgctg aacggctggc
    31621 tgctgtacca gacactggca tgccgcacct gggcgcgcgc gggcttctac caggcgagcg
    31681 gcgcatatgg cttccgcgac cagcttcagg atgcgatggc gctgggcatc gcccggccgc
    31741 agttgttgcg cgagcagatc ctgcgcgccg catcgcgcca gttccccgaa ggcgacgttc
    31801 agcactggtg gctgccgcac agtggccagg gcgtgcgcac ccacatctcc gacgatcgcg
    31861 tgtggctgtc cttcgtcact gcgcactaca tcgcgctcac cggcgaccgc gccattctcg
    31921 acgcacaggt gcctttcatc gagggccctc cgctgccgcg cgaacggcac gacgcattct
    31981 tcacgccctc ggtcggtgag cacaccgcga ctctgttcga acactgccgc ctcgggctcg
    32041 agggcgcgct gtacatgggc cagcatggct tgccgctcat aggcaccggc gactggaacg
    32101 acggcatgaa tcgcgtgggc gaacatggcc gcggtgagag cgtgtggctg gggtggttcc
    32161 tgcacgccac gctgcatgcg ttcgcgccgg tcgcgcaggc gcgcggcgaa ctcgcgctcg
    32221 ccaccgcctg gctggggcac gccaccaggt tgcgcgccgc cctcgaacaa catgcctggg
    32281 acggtgagtg gtatcgccgc ggctttttcg acgacggcac gccattgggc tcggcccaca
    32341 acgacgagtg ccagatcgac gccatcgcgc agtcctgggg cgtgatctcc ggcgccgcga
    32401 atccggcgcg aaccacgcgc gccatggccg cgctcgacgc gcaactgctg aaccgggatc
    32461 cgccgctggc catgttgttc acaccaccct tcgagaagtc ggcgcaggag cccgggtacg
    32521 taaaggccta tccacgcggc gttcgcgaaa acggcgggca gtacacacac gccgcagtgt
    32581 ggacctcgat cgccttcgcc atgcagggca acgcggaacg cgccatggaa gtgttcaaca
    32641 tgttgaaccc ggtgaagcgg actaccacgc gcgccgatgt tcagcgttac aaggtggaac
    32701 cgtacgtggt ggctgccgac gtatattccg aagcgccgca tgccggtcgc ggcggatgga
    32761 cctggtacac gggttcagcg ggctggttgt atcgcgccgg cctcgaatgg atcctgggat
    32821 tccgcgtgcg cgagaaccgc ctgacgctgg tgccttgtgt gccgtcggag tggcctggct
    32881 tcaccatcca ctacaaacat cgtggcacac cctacgaaat catcgtcgac aaccaacccc
    32941 atccgcacgc cctgccgctg ctggtgatcg acggcgacgt gcagaatgcc ggacgcaata
    33001 cggttgacct gatcgatgac ggcgccaaac acaccgtcca catgatgtgg cgcgcggcga
    33061 cagcgcaaga ggatttcgct gccacacgga gtagctgagc agcgtcgcga agctcaccca
    33121 ggcgagatac ggcgcgagta gccaggcggc gagccggcgt atcttcgcga acgacacgat
    33181 cgtcgccacg atgagcgcca gcagcaacac gatgtccacg aaagcgaacg cgccctggtg
    33241 ccaggcgaag
//
LOCUS       DASQJS010000362         4771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3052507, whole genome shotgun sequence.
ACCESSION   DASQJS010000362 DASQJS010000000
VERSION     DASQJS010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3052507"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VEW08_14285"
     CDS             complement(<1..416)
                     /locus_tag="VEW08_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYJ41957.1"
                     /translation="MKSGLPIDEILPALRAVLSAQRNAVVEAPPGAGKSTVVPIALLD
                     EPWVRGGKIVMLEPRRLATRAVATRMAMTLGESPGETVGYRMRLETRVSKRTRIEVVT
                     EGVFTRMLQSDPALEGVAAVLFDEFHERSLHADTGLA"
     gene            457..1305
                     /locus_tag="VEW08_14290"
     CDS             457..1305
                     /locus_tag="VEW08_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HYJ41958.1"
                     /translation="MRWRNTKASQNVEDYRGRRTGGGGGGMKFGIVGLVAIVGAYFLG
                     IDPRLIMGLLGGGTGQPTTEAPVDPSSPTDESGQFVQHVLGDTEETWTAVFANGGAQY
                     VAPRLALFDEGINTGCGSATSAVGPFYCPPDQKVYIDLTFFRQLETEFAAQGDFAKAY
                     VIAHEVGHHVQNLLGTADKVRAAQQRASESDANALSVRMELQADCFAGIWAHHADRAR
                     QVVESGDIDEALKAASAVGDDTIQKRVQGQVNQESFTHGSGAERQKWFRKGYGSGLMQ
                     DCDTFQ"
     gene            1311..1772
                     /locus_tag="VEW08_14295"
     CDS             1311..1772
                     /locus_tag="VEW08_14295"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HYJ41959.1"
                     /translation="MGSRQPVATGGCQCGAVRYAFYSPLENAHVCHCRMCQRATGGVF
                     AALAGTAPENFTWTHGTPAFFASSSLAKRGFCEKCGTPLSFKYDTANARMYTTIGSLD
                     HPERVTLVKQFGVESQLPWVKFCEAVPGERTAETAAAQEFLAKMQDHQSPL"
     gene            1842..2228
                     /locus_tag="VEW08_14300"
     CDS             1842..2228
                     /locus_tag="VEW08_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41960.1"
                     /translation="MKEREANQKYRQELFWSMLAYGAVLTGSIMLAKPMEPGMARTLL
                     LISPAIPMMLAVWAIARHFKRMDEFVRLRSLESLAVAAAVTAGLGITYGFLESAGFPK
                     LSMFWVWGVMGLVWGAHSCLRCVFSR"
     gene            2225..2419
                     /locus_tag="VEW08_14305"
     CDS             2225..2419
                     /locus_tag="VEW08_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018766546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYJ41961.1"
                     /translation="MKNSVRDLRTEHEWSQGDLADKLEVSRQTINAIETGRYDPSLPL
                     AFAIAKLFGKPIEKIFTPEK"
     gene            2422..3324
                     /locus_tag="VEW08_14310"
     CDS             2422..3324
                     /locus_tag="VEW08_14310"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ41962.1"
                     /translation="MKSKIFAGLALALSLLAPAFSAPAKFEGGALLVEQVSAKGPALI
                     FIPGLASGPWTWTQTTARLEGRYSIYLLTLPGFDGRKPAPDATLESLSRDLEALIRTR
                     KIDKPVLVGHSLGGTLSLAFAAGHSELIAGVVAVDGLPVFPGTEGMTGDRSPLAASAR
                     AQLAGQTPAQFAEYQRAYMKQVGSIDETIANSIAESSSRSDVGTTAEFAAQLLLLDLR
                     PKLPAIKVPVVEISPFYAPDFARINVDEAGKTGYYRALLAGIEKLDVVSISPARHFVM
                     FDQPEKFAVALDHALESMVESPSK"
     gene            3321..3734
                     /locus_tag="VEW08_14315"
     CDS             3321..3734
                     /locus_tag="VEW08_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HYJ41963.1"
                     /translation="MSAGYSGTPLSKKLGLKAGQRVCAPGAPTNYRKLLAPLPDGIEF
                     QARVTKTTGLVHVFTASKAELAKNLVAWLKVLAPDSVIWVSWPKKASKVPTDITEDTI
                     RAVALPMGLVDIKVCAVDETWSGLKLVLRKELRPK"
     gene            complement(3735..4145)
                     /locus_tag="VEW08_14320"
     CDS             complement(3735..4145)
                     /locus_tag="VEW08_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017217706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ41964.1"
                     /translation="MQSLGMVSLVVGDYDEALAFYVGKLGFTLVEDSFVPEQNKRWVV
                     IEPPGAGQSPSNQGAKLLLARGVNPEQTSRIGNQTGGRVFLFLYTDDFWRDFNAYKAK
                     GVEFAREPSEAPYGTVAVFKDLYGTLWDLVQLRS"
     gene            complement(4149..>4771)
                     /locus_tag="VEW08_14325"
     CDS             complement(4149..>4771)
                     /locus_tag="VEW08_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41965.1"
                     /translation="ARGWLAAAATAAVVAVAMLWLPFGTAPALAFAKVQEHFRDFRTL
                     RFEVEQRMNGRVIMKSRVSVTQDGNVRTDVGDDVTVIVNSTQRQVLTLMHRAHVAVPS
                     PLEKPATKEDALAWLDDIRDFQGEAKALPKARWIRGQRAQGWQLATAAGEIVLWANDE
                     GLPLEMSLGSSTPIQLSFDFEFNPSLDAKYFSTEIPAGYSRGESED"
BASE COUNT          857 a         1568 c         1496 g          850 t
ORIGIN      
        1 gccaggcccg tgtcggcgtg caggctgcgc tcgtgaaact catcgaacaa cacggcggcc
       61 acgccctcga gcgccggatc gctttgcagc atgcgcgtga acacgccctc cgtgacgact
      121 tcgatgcgcg tgcgtttgct cacgcgtgtt tcgaggcgca ttcgatagcc gacggtctcg
      181 ccgggcgatt cgcccagggt catcgccatg cgcgtcgcca ccgcgcgcgt ggcgagccgg
      241 cgcggttcga gcatcacgat cttgccgccg cgaacccacg gttcatcgag caacgcaatc
      301 ggaacaacgg tgctcttgcc cgcgccgggc ggcgcttcca cgacggcgtt gcgctgcgcg
      361 ctcagtacag cgcgcagcgc aggtaggatt tcgtcgatgg gcagacccga tttcatgagc
      421 cgcgtatttt agcgcctctc tgctaacatc cctggcatgc gctggcggaa taccaaagcg
      481 agtcagaacg tcgaagacta ccgtggacgc cgcaccggtg gcggtggcgg cggtatgaag
      541 ttcggcatcg tgggcctggt ggccatcgtc ggcgcctatt tcctcggcat cgatcctcgt
      601 ctcatcatgg gcctgctcgg cggcggcacc gggcaaccga ccaccgaagc gccggtggat
      661 ccgagctcgc ccaccgacga gagcggccag ttcgtccagc acgtgctggg cgacaccgaa
      721 gaaacctgga ccgccgtctt cgcgaacggc ggtgcacagt acgttgcacc gcgcctcgca
      781 ctgttcgacg agggcatcaa cacgggctgc ggctcggcca cgtcggctgt cgggcctttc
      841 tactgcccgc ccgatcagaa ggtgtacatc gatctcacgt ttttccggca gctcgaaacg
      901 gagtttgccg cgcagggcga cttcgccaag gcctatgtca ttgcgcacga ggtcggtcac
      961 catgtgcaga acttgttagg cacggccgac aaggtgcgcg cggcccagca acgcgcctcc
     1021 gagtccgatg ccaacgcgct gtccgtccgc atggagctcc aggcggattg cttcgcgggc
     1081 atctgggcgc atcatgccga ccgtgcgcgc caggtcgtcg agtcgggcga catcgacgaa
     1141 gctctcaaag cggcatcggc ggtcggcgac gacaccatac aaaaacgcgt gcagggccag
     1201 gtgaaccagg aatccttcac gcatggctcc ggcgccgaac gccagaagtg gttccgcaaa
     1261 ggctacggat ccggcctcat gcaggactgc gacactttcc agtgagcgcc gtgggttcgc
     1321 gccagcccgt cgccaccggc ggctgccagt gcggcgcggt tcgttacgct ttctactccc
     1381 cgctcgaaaa cgcgcatgtg tgccactgcc gcatgtgcca gcgcgccacg ggcggcgtgt
     1441 tcgcggcgct cgccggcacg gcacccgaaa atttcacctg gacgcacggc acaccggcgt
     1501 tcttcgccag ctccagtctc gccaagcgcg gcttctgcga gaaatgcggt acgccgctgt
     1561 ccttcaagta cgacaccgca aacgcgcgca tgtacaccac cattggctcg ctcgatcatc
     1621 ccgagcgggt aacgctcgtg aagcagttcg gcgtcgagag ccagttgccg tgggtcaaat
     1681 tctgcgaggc cgtgccgggc gagcgcaccg cggaaaccgc tgccgcccag gaattcctcg
     1741 cgaaaatgca ggaccaccag tctcccctgt aaaggaagct tgacaacgcg tttcggcacg
     1801 tctagcctgt aaagcatcct ttacagacac gggagagggc gatgaaagaa cgcgaagcca
     1861 accagaaata tcgccaggaa ctgttctggt cgatgctggc gtacggggcg gtgctgaccg
     1921 gctccatcat gcttgcgaag cccatggagc ccggcatggc gcgtacgttg cttctgatct
     1981 cgccggccat tccgatgatg ctcgcggtgt gggcaatcgc gcggcatttc aagcgaatgg
     2041 atgaattcgt gcggttgcgc agtctcgaga gtctggcagt cgccgcggcg gtcaccgcag
     2101 gccttggaat tacctacggc ttcctggaat ccgccggctt tccgaagttg agcatgttct
     2161 gggtgtgggg cgtgatgggt ctcgtatggg gggcgcattc atgtctgcgt tgtgtgttct
     2221 cgcgatgaaa aattcggtgc gtgatctgcg tacggaacac gagtggagcc agggcgacct
     2281 ggccgacaag ctcgaggttt cgcggcagac gatcaacgcc atcgaaacgg ggcgctacga
     2341 ccccagcctt ccgctcgcct tcgccatcgc gaagctgttc ggcaaaccga tcgaaaagat
     2401 ctttactccg gagaaatagc catgaaatcg aagattttcg cgggcctcgc gctcgcgctc
     2461 tccctgctcg cgcccgcgtt ttccgcaccg gcgaaattcg aaggcggcgc gctgctggtg
     2521 gaacaggtat ccgcaaaggg acccgcactc attttcatac ccgggctcgc cagcgggccc
     2581 tggacctgga cgcagaccac cgcgcggctc gagggaaggt attccatcta tctactgacg
     2641 ctcccgggtt tcgacgggcg caagccggcg ccggatgcga cgctcgagtc cctgtcgcgc
     2701 gatctcgaag cgttgatcag gacgcgcaag atcgacaaac ccgtgctcgt cggtcacagc
     2761 ctcggcggca cgctctcgct ggctttcgcg gccgggcatt ccgagctgat tgcgggagtc
     2821 gtggccgtcg acggcctgcc ggtgtttcca ggcaccgagg ggatgaccgg tgaccgcagc
     2881 ccgttggccg ccagtgcccg cgcgcaactg gccgggcaga cgcccgcaca gttcgccgaa
     2941 tatcagcgcg cctacatgaa gcaggtgggc agtatcgacg agacaattgc gaactcgatt
     3001 gccgagtcct cgagcaggag tgacgtcggc accaccgcgg aattcgcggc ccagctattg
     3061 ctgctcgacc tgcgcccgaa gctgccggcg atcaaggtgc cggtggtcga gatctcgccg
     3121 ttctacgcgc cggacttcgc acgcatcaac gtcgacgagg ctggcaaaac gggctactac
     3181 cgcgcattgc tcgcgggcat cgaaaagctg gacgtcgtct cgatttcacc ggcgcgccac
     3241 ttcgtcatgt tcgaccagcc ggaaaaattc gccgtggctc tcgaccacgc cctggaatcc
     3301 atggtcgaat ccccatcgaa atgagcgctg gatactcggg cacgccgctg tcgaagaagc
     3361 tgggactcaa ggccgggcag cgcgtctgcg cgcccggcgc cccgacgaac taccgcaagc
     3421 tgctggcgcc gctgcctgac gggatcgagt tccaggcgcg cgtgacgaaa accaccggtc
     3481 tcgtgcacgt cttcaccgca tcgaaggccg agctcgcgaa gaatctcgtg gcgtggctca
     3541 aggtgctggc ccctgattct gtcatctggg tgtcatggcc caagaaggcg tcaaaggttc
     3601 cgaccgacat caccgaagac accatccgcg ccgtcgcgct gccgatgggg ctcgtcgaca
     3661 tcaaggtttg tgcggtggac gaaacgtggt cggggctgaa gctggtgctg cgcaaggagc
     3721 tgcgcccaaa gtagctagct ccgtaactgc accaggtccc agagcgtccc gtacagatcc
     3781 ttgaacaccg ccaccgtgcc gtatggcgcc tcggacggct cgcgcgcgaa ttcgacaccc
     3841 ttcgccttgt aggcattgaa atcgcgccag aagtcatcgg tgtagaggaa caggaaaacc
     3901 cgcccacccg tctggttgcc gatgcgcgag gtctgttccg gattgacgcc gcgcgccagc
     3961 agtagtttgg cgccctggtt tgaaggactt tggccggcgc cgggtggctc gatgaccacc
     4021 cagcgcttgt tctgttcggg cacgaagctg tcttcgacca gcgtgaagcc gagcttgccg
     4081 acgtagaacg ccagcgcctc gtcgtagtcg ccaaccacca gtgacaccat gcccaggctc
     4141 tgcatcgttc agtcctccga ttcgccgcga ctatagcccg cggggatttc ggtgctgaag
     4201 tacttcgcat cgagcgacgg attgaactcg aagtcgaagc tgagctggat cggggtgctc
     4261 gaacccaacg acatctccag cggcaggcct tcgtcgttgg cccacaacac gatttcccct
     4321 gcggccgtcg ccagctgcca tccctgggcg cgctgaccgc ggatccaccg ggccttcggc
     4381 aacgccttcg cctcgccctg gaaatcacga atgtcgtcga gccatgcgag cgcatcctcc
     4441 ttggtcgcgg gcttttcgag cggtgacggc acggcgacgt gcgcgcgatg catcagcgtg
     4501 agcacctgcc gctgcgtcga gttgacgatc accgtgacat cgtcgccgac atcggtgcgc
     4561 acattgccgt cctgcgtcac gctgacgcgc gacttcatga tgacccggcc gttcatgcgc
     4621 tgctcgacct cgaagcgcag cgtgcggaaa tcgcggaagt gttcctgcac ctttgcgaag
     4681 gccagcgctg gtgcggttcc gaacggcagc cacaacatcg ccacggccac cacggccgcc
     4741 gtcgccgcgg ccgcaagcca gccccgcgcg c
//
LOCUS       DASQJS010000363        22738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2738232, whole genome shotgun sequence.
ACCESSION   DASQJS010000363 DASQJS010000000
VERSION     DASQJS010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 22738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22738
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2738232"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VEW08_14330"
     CDS             <1..710
                     /locus_tag="VEW08_14330"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HYJ41966.1"
                     /translation="PRGPGMERKDLLMANAQIFSAQGKAMNEVADRNIRVLVVGNPAN
                     TNAMIARANAKNLNPRNFTAMTRLDHNRAVSQLAEKTGSHVTRIQKMLIWGNHSSTQY
                     PDLNFCTVDGKSAHSLVDRKWYEDTFIPTVQQRGAAIIKARGASSAASAASAAIDHMR
                     DWHLGTSAGNFISMSVPSDGSYGIPEGVVYSYPVTCANGDYQIVQGLTVDEFSRKRMD
                     ATDKELREERDGVKELI"
     gene            complement(707..1465)
                     /locus_tag="VEW08_14335"
     CDS             complement(707..1465)
                     /locus_tag="VEW08_14335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ41967.1"
                     /translation="MSNAEMFSTVAREYANFRPGYPPELFAWLARISASREAAWDCGC
                     GSGQASVALAEHFAAVHATDVAPEQIAAAKPHPRVRYSVAPAEHSGLPAHSVDLVTVA
                     QALHWFDVDAFYAEAARVARPGALLAVWNYPRPQFEDAELDRRFLAFYTDVVGPYWPP
                     ERRHVESWYRTLPFPFEEVETPGFGIELSWNPEQVAGYVSSWSATVRYRKMLGKDPVP
                     MLRESLAAAWPANGASVPVRMPIGIRIGKLTPGS"
     gene            1606..4152
                     /locus_tag="VEW08_14340"
     CDS             1606..4152
                     /locus_tag="VEW08_14340"
                     /inference="COORDINATES: protein motif:HMM:NF009586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HYJ41968.1"
                     /translation="MLGAILVILSLIAGAAYVAKIGTGFTLLFIGAVVFMAYKRLPLI
                     AFTVVFTLLLAGYTYIAPPAGIWKGFLWVLLAATWLFNLRPLRKAVISRPFLKAYMRM
                     LPSMSQTEKEALEAGTVWWDGELFTGKPKFEKLLASKAPVLTAEEQAFIDGPCEELCR
                     MYDDWDVTHKRGDMPPAVWDYLKHKGFFAMIIPKKYGGLEFSAYAHSCVLVKLASRAG
                     LLAATTAVPNSLGPAELLNHYGTDEQKNYYLPRLARGEEVPCFALTHPRAGSDAASLP
                     DTGVVCKGMYQGKEVIGLKLNFSKRYITLAPIATVVGLAFRLYDPDKLLGSDKVDYGI
                     TCALIPRATPGLSIGRRHFPLNVPFQNGPLSGKDVFVPLDTIIGGPAMAGQGWRMLVE
                     QLSVGRCISLPSNATGGAKAGVFASGAYTRIRRQFNMSVGKFEGVEAVIARMVGYTYT
                     MDAARSVTAGAIDGGAKPSVPSAMLKYHVTEMGRQVANDAMDVHGGKGIQLGPRNYLG
                     RSYQVVPVAITVEGANILTRSLIIFGQGAVRCHPFVLKEMNAARNPDRKQGVDEFDSA
                     LFGHIGFTISNAVRSFVMALTHARFTRVPDVGDTRRYFQHIVRFSASFAFAVDVAMLT
                     LGGYLKKKENLSARLGDVLSAMYLASMVLKHHENQGRPADDLPIVEWACRNQLYKAQE
                     QLHDLLRNFPNRFLGWAMRALIFPGGRAYSAPSDRLGRKLADSVLTPSAVRDRLCHFV
                     YRTLEPGNPLGLLQEALVLSQTAEPIEKRIRVDGVKTGKVTALDVPGQITQALSVGII
                     SDTEAAMLRDYDRKVMELISVDDFESHELAAQAEAPPEVTPASVSSFQVA"
     gene            4163..5218
                     /locus_tag="VEW08_14345"
     CDS             4163..5218
                     /locus_tag="VEW08_14345"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HYJ41969.1"
                     /translation="MNSPPVPVVSPPEPPQPLTLVCANCHAALNGEYCASCGQRHEPH
                     VHTVAHFASEAFESVSHADSRLWRTLWYLLARPGYLTREFFAGRRVRYLPPFRLYLVI
                     SLVFFLTVGLPEGAAITFDDEPTAGRVEKMNATAEALEKEAGPGSDALRIAAARIREQ
                     AAREKATLDSADPVVGADGLSSKNAMTDFCEEFKNPDPTASENYAKLHAACAKITEDG
                     GRELAKVVVHNIPKAMFVFLPLLALSMKPLYWRPKRYYVEHLLFLVHNHAFIFLVLTL
                     LALFGMIPVVGEHLGLLDFAAWIYMLWYLFRGMRNYYGQGWWLTFLKYLQIGLSYISA
                     AVMVLVLLAMFSAITLG"
     gene            5289..5726
                     /locus_tag="VEW08_14350"
     CDS             5289..5726
                     /locus_tag="VEW08_14350"
                     /inference="COORDINATES: protein motif:HMM:NF025705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HYJ41970.1"
                     /translation="MKNRYLPRLALGAALILTATGVALAERTPSAAGAEVYIVSPKDG
                     AKVQGPVTVVFGLKGMGIAPAGIKFDNTGHHHLLVDADAPADLSKPLPADEHNIHFGK
                     GQTETTLTLPPGKHTLQLLLADSLHTPHDPAVISKKITIVIEK"
     gene            5766..7037
                     /gene="dinB"
                     /locus_tag="VEW08_14355"
     CDS             5766..7037
                     /gene="dinB"
                     /locus_tag="VEW08_14355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HYJ41971.1"
                     /translation="MSAPRAILHVDMDAFYASVEQRDRPELRGQPLIVGGTSNRGVVA
                     AASYEVRKFGVRSAMPIREALRRCPHAICVKPRMAVYREVSHQVFAVFHEYTPVVEGL
                     SLDEAFLDVTASLALKGDAVTIARNIKQRIRATTQLAASVGVAPNKLVAKIASELEKP
                     DGLTVINHDNMHAILDPLSVRRLPGLGRKLGERVEAAGIATFGQLRVAPDAVMWPIFG
                     RDSQRMRERASGIDERPVMSEWDEKSISAEETFFTDLADHGRMQAEVLRLADRAGTRM
                     RAQNLAAGCVAVKIRRADFTTFTRQKRFEPSTTDSRTIAKIAAELLEAWLVEQPRAKV
                     RLLGVGVNHLHAADQMDLFAAPAAAGGGNTGANALDAAVDLIRERFGNLAVRRGSALP
                     EARGEDEPAEDMSEKAPGKRRPRGHTPGTHR"
     gene            7168..8769
                     /locus_tag="VEW08_14360"
     CDS             7168..8769
                     /locus_tag="VEW08_14360"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYJ41972.1"
                     /translation="MYASFFGLNEKPFSITPDPRYLFLSERHAEALAHLVYGINEAGG
                     FIQLTGEVGTGKTTVVRSLLTQAPKHAEIALILNPRMTPAEFLLAICEELRLIVPQGS
                     ERSLKDLVDLLSRHLLKAHADGKRIVLVVDEAQNLAPEVLEQVRLLTNLETETQKLLQ
                     IILIGQPELRELLGRVELRQLAQRITGRYHLDPLSAEESSAYVRHRLRVAGATREIFS
                     NGALREIQRRSGGVPRLINIISDRALLGAFTEDRHAVSASVVRRAAGEVFGRTLHPHW
                     LPWTAAAGVGAIAIASAALLLPRFLVHAPAPVAAVPPPAPAPAPVAPAPDLDKLLGTY
                     GAETDPDNAFNKLFALWSLRYVAGTVDPCSQAQQAGIECLTQRGSLAQLRLFNRPAVL
                     NVIDSTGHAHQLVLAGLDDEHAQVDLGGAQREIGIGDLSRSWFGDYVLLWRPAAGGSQ
                     PLALGARNARVRWLRDSLRRVNGLPAEPVPNDRFDPELVTLVEEFQRRNRLAVDGIAG
                     VQTQVALDAALNDSATPFLRNSGGS"
     gene            8771..9505
                     /locus_tag="VEW08_14365"
     CDS             8771..9505
                     /locus_tag="VEW08_14365"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HYJ41973.1"
                     /translation="MSFILDALKKSENDRQRQSSPALFEVKVAAPRRNFPLWAVGLAV
                     LLGVNVLILLWFVLRQPAPASSAAPATAASEVAGTQSSNSTPPGMVTVPATSTYIPAG
                     GPPAVTSGGGNGSTDAGVVAPSPAPPLAGEPVLSGLEPSVPPDYDARDYRPAITPAQA
                     NAVAAARRDSVPSRDEVLAQGTQVPELRLDLHVYDPDPAKRFVFVNMRKLREGDSLPE
                     GVRLDVITQTGAKFSFRGTQFTLDAN"
     gene            complement(9502..9906)
                     /locus_tag="VEW08_14370"
     CDS             complement(9502..9906)
                     /locus_tag="VEW08_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYJ41974.1"
                     /translation="MTRLTTLLASALLGVACFVPANAADTVLVSQAELLKRLEQKDPD
                     LVVLDVRTADEFAAGHVPGARNVSHELLASRIDELVTARDKQVVLYCRSGRRTLLAED
                     VLRKAGFKRLGHLEGDYLAWETEKRPVERSSP"
     gene            complement(9922..10401)
                     /locus_tag="VEW08_14375"
     CDS             complement(9922..10401)
                     /locus_tag="VEW08_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ41975.1"
                     /translation="MLKIRPATRADAPVIAGLVRELADYEKLLDEAKATPEDFLRELE
                     SPNPVIHVLIAEWQGEPAGFALYFFNFSTFVGRPGLYLEDLFVRPAQRSHGIGRALLR
                     ALARIAKERGCGRMEWAVLDWNEPALRFYQSLDARQMKEWIIHRLTPVEIARLAAEA"
     gene            complement(10410..11342)
                     /locus_tag="VEW08_14380"
     CDS             complement(10410..11342)
                     /locus_tag="VEW08_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41976.1"
                     /translation="MRVRALIFTVLLASACFPASAAGPNGAQQKGAEAFLAALAGGNV
                     MAVAQELHPDEVEKLRTRLLTLFRAEASRSDNTYRSRLFGPGRSLAELESMTPVRFYA
                     ALSDRVRFRAREYEKVDWLGVVADGKVVYVVGKGVQPKERGAVKVLVTVALAQYGAQW
                     RAIIPSEVEAQLDDLIEGRVAAAPPPTGGAPGRSLVEERPAPLSPGISELLARAEASL
                     AEGHCEEYYSQFMSPNFNKATSKSAKKTLITACTNSESTRETMITTLRVVQELSPKYD
                     LGGSRAVFDVSGQGLPYDRFVLERDKDMRWYIAE"
     gene            11568..13724
                     /locus_tag="VEW08_14385"
     CDS             11568..13724
                     /locus_tag="VEW08_14385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYJ41977.1"
                     /translation="MLPQSEYESPMLANAAAPVAIAAITQDRALIGLLRSVIDPTNDL
                     ILVTSEAELKPHLNSRRVSVALLDSMFIEGDLGAMAERLRETWPDLVLVVVGTAEEQS
                     KVAGQITSGVVYRFLHRPVSAPRVRLFVDAALRRHEVENVERTLEQTRPDFSRMEKAA
                     KARTSGPSPIRAVLPGLIGAAIIIAGAGFWFARSSGDVQNAPPAPAAQVVSPEAEPPA
                     AEPAPAVASVPEQKTVELPPPADPEPAPVAAAPAAPPEEDVAERLRPAASPPAVVRAP
                     EEVAPRTPPPPVLTFEERLAQQLAQAESALQRGELASPPGRSAVDLFRGALELDPSNS
                     LAKGGLIRVADRLLTAAERTLTAGNVEDARRMVAVAESLTPATARGAFLTMQIEREHE
                     REALQRAKDSSTQDKLEKGATYLRLANARLRSGALIEPSEDNARFYLEAASQLLPDDD
                     AGLAETRRGLQRALLDRASAAASDGNAAETERWLANADDAGAPRADIAAIRRSLKDTE
                     ISARAAKMTTLTQLFTSALAANRLLQPPSDNAKTHLLGMIDLDGNHPAVASARQGLGN
                     ALLGEFRGAIARGDLTAADTWLNEARTVGFTGNELRAAEGDLAGARGKANSGSSVVGE
                     RALERIEYVAPKFPLVARNRGSTGSAGSTGWVELEFTVLPDGSTGNIVVTNSNPRRTF
                     DASAVTAVGQWRYKPLIRDGKPVEQRVAVRIRFADQ"
     gene            13725..15401
                     /locus_tag="VEW08_14390"
     CDS             13725..15401
                     /locus_tag="VEW08_14390"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYJ41978.1"
                     /translation="MDAPRTQRFLIVENDAKYAAWLQHTVSAGWPNDSIVIMDWTSFG
                     RVRTAMTVRDYDIVMLALVFDEGIEEPTTDSFDWIRKLRSQPGFPEMVILAEGGSELA
                     AIRTLRLGAADYLPKRLLTPPRLQRSLKLTLRAIEKEAQRRAERSAQTAAKAETDKHA
                     ESLTGSVKVAPPTASPPAATPSHISTHTGASLAKAAAQAAAQTSVPPKPAAAQMPPVA
                     PAPPRAHIATHTGASLAVAAKAALAAHAPPKLEPSNKEAPKETKPIAPESHQIPGYTI
                     LQKIGESEAAAVYLAIAEDLGHNVALKISKRKHSGNVDTAASDTGQRSIMFQREFEAI
                     AALDHPSIIDLFDYGIHEGIEYLAMEYFPCGDLKARLQNPLTSDEAIAFLREIARSLK
                     VVHDAGIIHRDLKPPNVMLRDNGNVVLIDFGLARSLLSGDGSTRTGVLRGSPYYMSPE
                     QAQGEALDARTDLYSLGVILYEMLAGKKPYLGASAIDVLQQHVMAPVPELPVHHLGYQ
                     PLLERLMSKSREQRIASCDELLAALEQMSHAMSDSGIIDPETLVSAGAGS"
     gene            15411..17561
                     /locus_tag="VEW08_14395"
     CDS             15411..17561
                     /locus_tag="VEW08_14395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ41979.1"
                     /translation="MEFSTFPIVALAMALICLIGVTIYAITQRGSLRSRDEQLVVARA
                     QAYAMLELSQAGVLFLDREHKLMGEASAAAPELLGHTCGAGTAFVQVIADLVDVQVRR
                     QAVAFLESMWKAEPASVVDATQNPLAKVHSGARHLAIRFARLVVDGRVHHIIVSIERI
                     SAPRLMPLTIEVPALNEETFAKRLAGETGTRPALNIASMAANTELVTAAPESAVAAAP
                     PAPAAPAAPAPVPSAAHASPPPVAPMSTGEFELGPMDVTEDSIPSLPVLSDAAAAAMA
                     KLAPASETVDKTQKIATLSAASLPSNVPASDAVTATMRRLDEEEKQLATTITSPDVAQ
                     EASKPKPTTPELDPSGIIDPPDPRLSEVLREVMHIDAGRLETFLAEAREKAGQLRAIM
                     KLPAREPQAFREKLVLILELIRGIHVRAERIPVPSVCERADRFAAALNALRDKQTLSG
                     NDFLPLAVKLDDLLSHLAILGEVVTRLREWRVQNGESGAPSIDATQRSATATGTTIRQ
                     PLMRDAAPSATGITKAARLSDLSQESLDEMALFLADMYSKRVSLVVVGLEDVPTHYRR
                     SVEKILGQLIHNAIRHGVENPADRAVLDKPEVGTIAVQFARMGSDGYQLSVQDDGRGL
                     DHDKIRAEAVRQGVLSAEVAANIDTRKLAGLIFRPGFTTVGEGGARGIGMDVVRDLVT
                     KAGGRVGIATKPGEYTRFRISLPHEKKASDAAVA"
     gene            complement(17566..17808)
                     /locus_tag="VEW08_14400"
     CDS             complement(17566..17808)
                     /locus_tag="VEW08_14400"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HYJ41980.1"
                     /translation="MRGAETPLCGTGRSNMPSSVIRSYRYDPQQKRLELVFVSGRRYR
                     YHDVPEETYSAMSRAFSKGEFFNAHIRNQFRHTRES"
     gene            complement(17851..19161)
                     /locus_tag="VEW08_14405"
     CDS             complement(17851..19161)
                     /locus_tag="VEW08_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41981.1"
                     /translation="MSQKFDDFLARFVRIEPGEKRAVFTAFALMFCVMGGYFSVRPIR
                     ETVGTILGEAITSDTWIWTAVFAIAIIPVYGFLVARVSRATLVPAIYGSVAVALAATG
                     LVFRADPNNMIVGRVFYVGISVINLFLISVFWSFLLELFRSEQTKRLFGFIAAGGTTG
                     ALVGPFLTDRIVIHVGISGILFFGAAMFSIAIFCQRALLAEMRHHADLAAAADAAAGR
                     ATQAQRGVGGNPFAGVSIVLKSPYLMGIALFVIMISTANTILYFEQLRLVKDMFDDPE
                     QRTQMFARIDYIVQTLTVISQVLITGRLAQKLGVTALLVILPLLIMGGFLILASMGTF
                     LVLATVMVLRRWGEYAFIRPGREMLWSRLDTETKYKAKNFVDVTVYRFSDAVVAQVQD
                     GLKAGGMNPAQAALLGAGVCAAWAVNGWWLGRRFDKEKEQKSVA"
     gene            complement(19207..20604)
                     /locus_tag="VEW08_14410"
     CDS             complement(19207..20604)
                     /locus_tag="VEW08_14410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYJ41982.1"
                     /translation="MQLEFFGAAGEVTGSCHILRVAGRDLLLDCGMIQGGHDAPARNR
                     LPFPFEAAAIDAVVLSHAHIDHCGRLPLLVKRGYRGPIYTNKACADLVPILLRDSASL
                     ALRDSERAARHGERIEPLYDLDDVETTLRQIVPMPYDTQRGLLSGVVVRVRDAGHILG
                     SSSVELWASEGETRRKLVFSGDLGQYDTPILQDPYKFESADLVLMESTYGNRLHRGRE
                     DTERELGGILAQAERDGGNVIVPAFAIGRSQELLYLLAKHYAEWHLARWKVFLDSPMA
                     IAASRVYWNNPERFDEETTRLRENFRGMPPLPNLVLSESSEDSRAINHVRSGALIIAG
                     SGMANGGRVLHHLKHNLERPECHVVIVGFQAPGTLGRQLVDRQPEVRIHGQRVRCLAQ
                     VHTLGGLSAHGDQDDLLRWYDSFAGRPPVYLVHGEVPSAEALAVKLREHGAVATVSRP
                     GLTIDLANLPALSVD"
     gene            20654..>22738
                     /locus_tag="VEW08_14415"
     CDS             20654..>22738
                     /locus_tag="VEW08_14415"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYJ41983.1"
                     /translation="MDTKSSSGFDRRTFLTAAVATGVVGGGLVLGIRYANRREEAAGA
                     AASGRTLAPNAFVRVAPDDTITVVIGKAEMGQGIYTGLAMALAEELDVDPKRVVVELA
                     GADPAFNVPGMPIQITGGSMSTSTTYTQLREAGARARAMLLAAAAQKWNVDAAELTTF
                     DGKVNRGSKSLTYGSLADAASQLPVPEKVTLKDPAQFRYLGKPIKRLDAPMKVDGSAK
                     FGLDMRVPGMVFAVIARPPVIGATLGNVDDSAARAVSGVVDVKTIPAGVAVYATNTWA
                     AKRGRDALKLDWNEGPEANYSTTAQRREYQRLLGKPGAVARISGDVKTALSSAAKRMD
                     VEYELPYLAHSPMEPLNCLADVRSDGCDLWLGTQMQTTDRDAAARELGLEPSKVTLHN
                     MFLGGGFGRRAQRNNEVVIEAVQLSKAIGKPVLSVYTREDDVRGLSYRPHVMSRVRGG
                     VDAAGMPVAWQQTIVSQGVLRGGWTDAFIPKGQAYDQSSVEGASDMPYDIPNVQVDTH
                     EGTSKVPVLWWRSVGHSHTGFTVNCALDELAVLGGQDPVELRRKLLAGKPRVLAVLNA
                     VAESSNWAGGAPAGRGRGVALQESFGSIVAEVAEVSVEDGAVRVHKVWCAIDCGFAVH
                     PEGVKAQMESAINYGLTAALYGEITFTGGKVDQSNFHDYQILRINEAPEIHVTIVNSG
                     EQMGGAGEPGTPP"
BASE COUNT         4153 a         7666 c         7135 g         3784 t
ORIGIN      
        1 gcccgcgcgg tcccggcatg gaacgcaagg acctgctgat ggccaatgcg cagatcttct
       61 ccgcccaggg caaggccatg aacgaagtgg cagaccgcaa catccgcgtg ctggtggtcg
      121 gcaatccggc caataccaac gccatgatcg cgcgcgccaa tgcgaaaaat ctgaacccgc
      181 gcaatttcac ggcgatgacg cgcctcgatc acaaccgcgc ggtctcgcaa ctcgccgaaa
      241 aaaccggcag ccacgtcacc aggattcaga agatgctgat ctggggcaat cactcctcga
      301 cgcagtaccc ggatctgaac ttctgcaccg tcgacggaaa atcagcgcac tcgctggtcg
      361 atcgcaagtg gtacgaagac accttcatcc ccaccgtgca gcagcgtggc gccgccatca
      421 tcaaggcgcg cggcgcctcg tcggcggctt cggccgcgtc ggccgccatc gaccacatgc
      481 gcgactggca cctcggcact tccgccggca acttcatcag catgtccgtg ccctccgacg
      541 gcagttatgg catcccggaa ggcgtggtgt attcgtaccc ggtcacgtgc gccaatggcg
      601 actaccagat agtccaggga ctcaccgtcg atgaattctc gcgcaagcgc atggacgcca
      661 ccgacaagga actgcgcgaa gagcgcgacg gcgtgaagga actgatctaa ctaccgggcg
      721 tcagcttgcc gatgcgtatc ccgatcggca tgcgcaccgg cacgctggcg ccgtttgccg
      781 gccaggcggc ggcgagggat tcgcgaagca tcggcaccgg atcctttccc agcatcttgc
      841 gataacgcac ggtagcggac cagctgctca cgtaacccgc cacctgctcg ggattccacg
      901 agagttcgat tccgaaaccc ggcgtttcca cctcctcgaa gggaaacggc agggtccggt
      961 accacgattc gacgtggcgg cgttccggtg gccagtacgg gccaaccacg tcggtgtaga
     1021 aggcaaggaa gcggcgatcg agttcggcat cctcgaactg aggccgcggg taattccaca
     1081 cggcgagcag cgctcctggt cgcgcgacgc gcgcagcttc ggcataaaac gcgtccacgt
     1141 cgaaccaatg cagcgcctgc gccacggtga ccagatccac ggaatgggcg ggaagacccg
     1201 aatgctcggc cggtgcgacc gaatagcgaa cacgcggatg aggcttcgcg gcggctatct
     1261 gttcgggcgc cacatcggtg gcatgaaccg cggcaaagtg ttccgccagg gccacgctcg
     1321 cctgaccgct gccacagcca caatcccacg ccgcctcacg tgaagccgaa atgcgcgcga
     1381 gccacgcgaa cagctcgggc ggataaccgg gcctgaagtt tgcgtactcc cgcgccaccg
     1441 tggaaaacat ctcggcattc gacatgccgc tattgttacg ccgacggcgc caccggccca
     1501 agtatgggct ggcgacgccg cgtcaatgga cagacataaa gtcacccgcg cgcggtgcgg
     1561 tgcggaaacg actatagttt cgccatcgcg aatcgagggc ctcccatgct cggcgcaatc
     1621 cttgtcatcc tttccctgat cgccggcgcc gcctacgtgg cgaagatcgg cacgggtttt
     1681 actctgctct tcataggcgc cgtcgtgttc atggcgtaca agcgcctgcc actcatcgcc
     1741 ttcacagtgg tgttcacgct gctgctggcc ggctacacct acatcgcgcc ccctgccggc
     1801 atctggaaag gcttcctgtg ggtgctgctc gcggcgacct ggctgttcaa cctgcgcccg
     1861 ctgcgcaagg cggtcatctc gcggcccttc ctcaaggcct atatgcgcat gttgccctcg
     1921 atgtcgcaga ccgaaaagga agcgctcgag gccggcacgg tctggtggga tggggagctc
     1981 ttcaccggca aacccaaatt cgaaaagttg ttagccagca aggcgccggt gctcacggcc
     2041 gaggaacagg cgttcatcga cggtccctgc gaagagttgt gccgcatgta cgacgactgg
     2101 gacgtcacgc acaagcgcgg cgacatgccg cctgcggtgt gggactacct caagcacaag
     2161 ggtttcttcg cgatgatcat cccgaagaaa tacggtggcc tcgagttctc cgcctacgcg
     2221 cactcctgcg tgctggtcaa actcgccagc cgcgcgggat tgctggctgc caccaccgcg
     2281 gttccgaatt ccctcggccc cgccgagctg ctgaatcact acggcaccga tgaacagaag
     2341 aactactacc tgccgcgcct ggcgcgtggc gaggaagtgc cctgcttcgc gctcacgcat
     2401 ccacgcgccg gttccgacgc cgcgtcgctg ccggataccg gcgtcgtttg caaaggcatg
     2461 taccagggca aggaagtcat cgggctcaag ctcaatttct ccaagcgcta catcacgctc
     2521 gcgcccatcg cgaccgtcgt cggcctggcg tttcgtctct acgatccgga caagttgtta
     2581 ggtagcgaca aggtcgatta cggcatcacc tgcgcgctga tcccgcgcgc cacgccgggc
     2641 ctcagcatcg gccggcgcca ctttccgctc aacgtgccgt tccagaacgg tccgctgtcg
     2701 ggcaaagacg tgttcgtgcc tctcgacacc atcattggcg gccctgcgat ggccggccag
     2761 ggctggcgaa tgctggtcga gcagctttcg gtaggccgct gcatctcgct gccgtcgaac
     2821 gctaccggcg gagccaaggc gggtgtgttc gcctcggggg catacacgcg catccgccgg
     2881 cagttcaaca tgtcggtggg caagttcgag ggcgtggaag ccgtcatcgc acgaatggtg
     2941 ggatacacct acaccatgga cgcggcgcgc tccgtcaccg cgggcgccat cgatggcggt
     3001 gcaaagccgt cggtgccttc cgcaatgctg aagtatcacg tcaccgagat gggccggcag
     3061 gtggcgaacg acgccatgga cgtgcacggc ggcaaaggca ttcagttagg ccctcgcaac
     3121 tatctcggcc gcagctacca ggtagtcccg gtggccatca cggtcgaagg agcgaacatc
     3181 ctcacgcgct ccctcatcat cttcggccag ggcgcagtgc gctgccatcc gttcgtgctg
     3241 aaagagatga acgcggcgcg caatccggac cgcaagcagg gcgtcgacga attcgactcc
     3301 gccttgttcg gtcacatcgg gttcaccatc agcaacgcgg tgcgctcctt tgtcatggcg
     3361 ctaacgcacg cgcgcttcac ccgcgtgcca gacgttggcg acacccgacg ctatttccag
     3421 cacatcgtgc gcttttcggc gtctttcgcc tttgcagtgg acgtcgccat gctcacgctg
     3481 ggcggctacc tcaagaagaa agaaaacctt tccgcgcgtc tgggggacgt gctctcggcg
     3541 atgtacctcg catcgatggt gctcaagcac catgaaaacc agggacgtcc cgcggacgat
     3601 ctgccgatag tcgagtgggc ttgccgcaac cagctctaca aggcacagga gcagctgcac
     3661 gatttactgc gcaacttccc caatcggttc ctcggctggg ccatgcgcgc gctcatcttt
     3721 ccgggcggcc gcgcctattc cgctccgagt gaccggctcg gccgcaagct cgccgactcg
     3781 gtgttgaccc cgagcgccgt tcgcgatcgc ctgtgccatt tcgtctaccg cacgctcgag
     3841 cctggcaatc cgctgggact gttgcaggaa gccctggtgc tgtcgcagac cgccgaaccc
     3901 atcgagaagc gcatccgtgt cgacggtgtg aagaccggca aggtgaccgc cctcgatgtt
     3961 cccgggcaga tcacccaggc cctgagcgtc ggcatcatct ccgacaccga ggcggcgatg
     4021 ctgcgcgact acgatcgcaa ggtcatggag ctcatcagcg tcgacgactt cgaatcgcac
     4081 gagctggctg cgcaggcgga agcaccgccg gaggtgacgc cggccagcgt ctccagcttt
     4141 caggtcgcct gaaccggaga cgatgaactc acctcccgtc ccggtagtta gcccgccgga
     4201 gccaccccag ccgctcacgc tggtgtgcgc caactgccac gcggcactta acggcgaata
     4261 ctgcgcgagc tgcggacagc ggcacgagcc gcacgtgcac acggtcgcgc atttcgctag
     4321 tgaagcgttc gaaagcgttt cgcacgccga ttcacggcta tggcgcacgc tctggtacct
     4381 gctcgcgcgt cccggctatc tcacgcggga gtttttcgcc gggcggcgcg tgcgctacct
     4441 gccgccattc cggctctacc tcgtgatcag cctggtgttc ttcctgaccg tggggttgcc
     4501 cgaaggcgca gccatcactt tcgacgatga gccgaccgcc gggcgcgtcg agaaaatgaa
     4561 cgcgacagcc gaagcgcttg aaaaggaagc gggcccggga agcgacgcac tcaggattgc
     4621 ggcagccagg atcagggaac aggccgcgcg ggaaaaggcg acgctcgact ccgcggatcc
     4681 ggtagttggc gccgacggcc tctcgagcaa gaacgcgatg accgacttct gcgaggaatt
     4741 caagaatccg gatcccaccg ccagcgagaa ctacgccaag ctccatgcgg cctgcgccaa
     4801 gatcaccgag gacggaggcc gggagctcgc aaaggtggtc gtgcacaaca tcccgaaggc
     4861 gatgttcgtg ttcctgccgt tgctcgcgct gtcgatgaag cctctgtact ggcgcccgaa
     4921 gcgctactac gtcgaacacc tgttgttcct ggttcacaac cacgccttca ttttcctggt
     4981 gctgacgctg ctcgcactgt tcgggatgat tcccgtcgtg ggcgagcacc tggggctgct
     5041 cgatttcgcc gcgtggatct acatgctctg gtacctgttt cgcggcatgc ggaactacta
     5101 cggccagggc tggtggctca cttttctgaa gtacctgcag atcggcctct cctacatctc
     5161 ggcggccgtg atggtcctgg tactgctggc gatgttcagc gccatcaccc tcggctagat
     5221 ctctgcactg cttgcgtgtc cggttcccgc gggtctaagt tgcaccccca cgcccaagga
     5281 gcctgccgat gaaaaaccga tacctgccgc gtctcgcgct cggtgctgca ctcatcctca
     5341 ctgccaccgg cgtcgctctc gccgagagga caccttcagc tgccggcgcc gaggtttaca
     5401 tcgtctcccc gaaggacggt gcgaaggtcc aaggtcccgt gaccgtggtc ttcggcctca
     5461 agggcatggg tatcgcgccc gccggcatca agttcgacaa cacggggcac caccacctgc
     5521 tcgtcgatgc cgacgcgccc gccgatcttt cgaagccgct gcccgccgat gaacacaaca
     5581 ttcatttcgg caagggccag acagaaacca cgctgacact gccgcccggc aaacacacgc
     5641 tgcagctact gctggcggat tcgctgcaca caccgcacga cccggccgtg atttcgaaga
     5701 agatcacgat cgtcattgag aagtaagccg tcggaaagca gacacggacg aggtgagccg
     5761 gcctcatgag tgcaccgcgc gccatcctgc atgtcgacat ggacgctttc tatgcgtcgg
     5821 tggagcagcg cgaccggccc gagttgcgcg gccagccgct catcgtcggc ggcaccagca
     5881 atcgcggcgt ggtcgcggcg gcgagctacg aagtgcggaa gttcggggtt cgttccgcca
     5941 tgccgatccg cgaggcgctg cgccgttgcc cgcacgccat ctgcgtcaag ccacgcatgg
     6001 cggtgtatcg cgaagtctcg caccaggtgt tcgccgtatt ccatgaatac acgccggtgg
     6061 tcgaaggcct gtcgctcgac gaggcttttc tcgatgtgac cgcgagcctg gcgctcaagg
     6121 gcgacgccgt gaccatcgcg cggaacatca agcagcggat tcgcgccacc acgcagctgg
     6181 cggcctcggt cggcgtggcg cctaacaaac tggtggcgaa gatcgcttcc gagctggaga
     6241 agccggacgg actcaccgtg ataaaccatg acaacatgca cgcgatcctg gatccgctct
     6301 cggtgcgccg actgcccggc ctcggccgga agttaggcga acgggtcgag gccgcgggaa
     6361 tcgccacgtt cgggcagctc cgggtggcgc ccgatgccgt gatgtggccg atcttcgggc
     6421 gggactcgca gcgcatgcgc gaacgcgcct ccggcatcga tgagcgaccg gtgatgtccg
     6481 agtgggacga gaagtcgatc agcgccgagg aaacgttttt caccgatctc gccgaccacg
     6541 gcaggatgca ggccgaagtg ctcaggctgg ccgaccgcgc cggcacgcgc atgcgcgcgc
     6601 agaatctcgc tgccgggtgc gttgcggtga agatccggcg ggccgacttc accaccttca
     6661 cccggcagaa gcgattcgag ccgtccacca ccgactcacg cacgatagcc aagatcgccg
     6721 ccgagctgct cgaggcgtgg ctcgtcgaac agccgcgcgc gaaggtgcgg ttgttaggcg
     6781 tcggggtgaa tcacctgcac gccgccgacc agatggacct gttcgccgca cccgcggcgg
     6841 ccggcggtgg gaacaccggc gccaacgccc tggacgccgc cgtggacctg atccgcgagc
     6901 ggttcggcaa cctcgccgtg cgccggggca gcgcccttcc cgaggcgcgt ggcgaggacg
     6961 aaccggcgga ggatatgtcc gaaaaagccc cggggaagcg taggccacgc gggcacacgc
     7021 ctggcactca ccggtagtcg gcacctgttg agccgcgttg gtagcccggg cggaaaagta
     7081 tttttcaggc gtgccgccac tcttaacgcc gccgaagcgg cggcgtacgt ttaatatcca
     7141 gaaccagcct ccgggccgtg aataaccatg tacgcctcct ttttcggact caacgagaag
     7201 cccttctcga tcacccccga cccgcgctac ctgttcctct cggagcggca cgcggaagcc
     7261 ctggcgcacc tggtttatgg catcaacgag gcgggtgggt tcatccagct caccggcgag
     7321 gtcggcacgg gcaagaccac ggtggttcgt tcgctgctca cccaggctcc caaacacgcc
     7381 gagatcgcgc tcatcctcaa cccgcgcatg accccggcgg agttcctgct cgccatctgc
     7441 gaagaactga ggctgatcgt gccccagggg tccgaacgca gcctcaagga tctcgtggac
     7501 ctgctaagcc ggcacctgct caaggcgcac gccgacggca aacgcatcgt gctggtggtc
     7561 gacgaagcgc agaacctggc gccggaagta ctcgaacagg tacggctgct caccaatctc
     7621 gagaccgaga cgcagaagct gctgcagatc atcctcatcg ggcagccgga gctgcgcgag
     7681 ttgttaggcc gcgtcgagct gcggcagctc gcccagcgca tcaccgggcg ttatcacctc
     7741 gatccgctgt cggccgaaga atcctccgcg tacgtgcgcc atcgcctgcg ggtcgccggc
     7801 gccacgcgcg aaatattctc caatggggca ttgcgggaga ttcagaggcg ctcgggaggc
     7861 gtgccacgcc tcatcaatat catcagtgat cgtgccttgt taggtgcctt caccgaagac
     7921 cgccatgcgg tcagcgccag cgtcgtgcgc cgggctgccg gtgaagtttt cggccgcact
     7981 ttgcatccgc actggctgcc gtggaccgcg gcggcgggcg tgggcgcgat tgccatcgcg
     8041 agcgcggcac tgctgctgcc gcgattcctg gtacacgcgc ccgcaccggt ggccgccgtg
     8101 ccgccacctg cgcccgcgcc ggcacccgtg gcgcccgcac ccgatctcga caagttgtta
     8161 ggcacctacg gcgctgaaac cgatccggac aatgccttca acaagctgtt tgcgttgtgg
     8221 agcctgcgct acgtcgccgg cacggtcgat ccctgcagcc aggcgcagca ggccggcatc
     8281 gaatgcctga cgcaacgcgg ctcgctggca caattgcggc ttttcaaccg ccccgcggtg
     8341 ctcaatgtca tcgactccac cgggcacgct caccagctcg tgctcgccgg tctcgacgat
     8401 gaacacgcgc aggtggacct gggcggcgcg cagcgcgaga tcggcatcgg ggacctctcg
     8461 cgaagctggt tcggcgacta tgttctcctg tggcggccgg ccgccggcgg ctcgcagccg
     8521 ctggccctgg gtgcgcgcaa tgcgcgcgtg cgctggttgc gcgacagcct gcgccgggta
     8581 aacgggcttc cggcggagcc ggtgccgaac gatcgcttcg acccggagct cgtgaccctg
     8641 gtggaagaat tccagcgcag gaatcgcctg gcggtggatg gcatcgccgg cgtccagacc
     8701 caggtcgcgc tcgacgcggc gctcaacgat tccgccacac ctttcctgcg caatagcggt
     8761 ggtagctgac atgtcgttca ttctcgatgc actgaagaaa tccgagaacg accggcagcg
     8821 gcaatcgtca ccggcactgt tcgaggtcaa ggtcgccgcg ccgcgccgca acttcccgct
     8881 ctgggccgtg gggctcgcgg tgttgttagg cgtcaatgtc ctcattcttc tctggttcgt
     8941 gctgcgccaa cccgctcccg cgagctccgc cgcgccggcc accgccgcca gcgaagtcgc
     9001 cggcacgcaa tcgtcgaact cgacgcctcc cggcatggtc acggttcccg ccacgtcgac
     9061 gtacattccc gcgggcggtc cgccggccgt cacgagcgga ggcggtaatg gcagcaccga
     9121 cgctggcgtc gtggcgccga gccccgcccc accgctcgcc ggggaaccgg tgttgtcagg
     9181 gctggaaccc tcggtcccgc ccgactatga cgcgcgcgac tatcgccccg cgattacgcc
     9241 cgcgcaggcg aacgccgttg ccgcggcacg gcgcgattcg gtgccctcgc gcgacgaagt
     9301 gctggcgcag ggcacgcagg tgccggaatt gcgactcgac ctgcacgtgt atgacccgga
     9361 ccccgcgaag cgattcgtgt tcgtcaacat gcgcaagctc cgcgagggcg attccctgcc
     9421 cgaaggtgtg cggctcgatg tgatcacgca gaccggggcg aaattttcct tccgcggcac
     9481 gcagttcacg ctggacgcga actaaggcga agaccgctcg acgggccgct tttcggtctc
     9541 ccacgcgagg tagtcgcctt cgagatgccc caggcgcttg aagccggcct tgcgcagcac
     9601 gtcctcggcc agcaacgtgc gccggccgct gcggcagtac aggaccactt gcttgtcgcg
     9661 cgcggtcacg agttcgtcga tgcgagacgc gagcagctcg tgcgatacgt tgcgcgcacc
     9721 cggcacgtgg ccggccgcga attcgtcagc cgtgcgtacg tcgagcacca cgagatccgg
     9781 gtctttctgc tcgaggcgct tgagcagctc tgcctgggaa accagcacgg tatcggcggc
     9841 gttggccggc acgaagcacg caacacccag caaggcgctg gccaacaatg tagtgagacg
     9901 ggtcatgaag ttctcctcga ttcaggcttc ggctgccagg cgtgcgatct cgactggcgt
     9961 gagacggtgg atgatccatt ccttcatctg ccgcgcgtcc agcgactggt agaaacgcag
    10021 cgccggttcg ttccagtcga gcacggccca ctccatgcgt ccacacccgc gttccttggc
    10081 gatgcgggcc agcgcccgca acagcgctcg cccgatgccg tggctgcgct gcgcgggccg
    10141 tacgaacaga tcttcgagat acaggcccgg ccgccccacg aacgtcgaaa agttgaagaa
    10201 atacagcgcg aaacccgcgg gttctccctg ccactccgcg atcagcacgt gaatcacggg
    10261 gttgggggat tcgagctcgc gcaggaaatc ctcaggagtc gcctttgcct cgtcgagcag
    10321 cttttcgtag tccgcgagct cgcgcaccag gcccgcaatg accggtgcgt ctgcccgcgt
    10381 cgcgggccgg attttcagca tttgttccat tactccgcga tgtaccagcg catgtctttg
    10441 tcacgctcca ggacgaagcg atcgtagggc aggccctggc cgctcacgtc gaagacggcg
    10501 cgagaaccac ccaggtcgta tttcggcgag agctcctgga ccacgcgcaa cgtggtgatc
    10561 atggtttcgc gcgtgctctc gctgttggtg caggcggtga tcagcgtctt cttggcggat
    10621 ttcgaggtcg ccttgttgaa gttggggctc atgaactggc tgtagtactc ctcgcagtgt
    10681 ccctccgcga gactggcttc ggcacgcgcc agcagttcgc tgatgcccgg ggacagcggc
    10741 gccggccttt cctcgaccag cgacctgccc ggggcacctc ccgtaggcgg cggcgcggcg
    10801 gcaacccggc cttcgatgag gtcatcgagt tgcgcttcga cttcgctggg gatgatcgcg
    10861 cgccactgcg cgccgtactg agccagcgcg acggtgacca ggactttcac cgctccgcgc
    10921 tctttcggct gcacgccttt gccgacgacg tacaccacct ttccgtcggc gactacgccg
    10981 agccaatcta ctttctcgta ctcgcgtgcg cgaaagcgca cccgatccga aagcgcggca
    11041 tagaaacgca cgggggtcat gctctcgagc tcggcgagcg accggcccgg tccaaacaac
    11101 cgcgaacgat aggtgttgtc ggatcgcgaa gcttcggcgc ggaacagcgt gagcaggcgc
    11161 gtgcgcagct tttccacttc atcgggatgc agctcctggg ctaccgccat cacgttgccg
    11221 cccgccagtg cggccagaaa tgcctctgcg cccttctgct gggcgccatt cggacccgcg
    11281 gcggacgcgg gaaagcatgc tgaagccaac aacacggtga aaatcagggc tctaacgcgc
    11341 attgggcact ccattcgaat acggcggcgg cgatcataac cgggccggta atggccgccc
    11401 caactgtgag agtggtctcg ttaccagaca ttggtggcgg gacgcagttc acggattacc
    11461 cgcgcattgg gcctctgggc ggccttcccc gataaactgt catatcttgt taaagtgctt
    11521 tcgcaccctt tgttttcgct gctttttgcg ggtccgccac taccgtcatg ttgccgcagt
    11581 cggaatacga atcgcccatg ctcgccaatg ccgcggcgcc ggtggcgatt gccgcgatta
    11641 cgcaggatcg cgcgctgatc ggcctgctgc gcagcgtgat cgacccgacc aacgatctca
    11701 tcctcgtcac ctctgaagcg gaactcaagc cgcatctgaa cagccgtcgc gtgtccgtgg
    11761 cgctgctcga cagcatgttc atcgaaggcg acctcggcgc gatggcggag cgtctgcgcg
    11821 aaacctggcc ggatctcgtg ctcgtggtgg tcggcaccgc cgaagaacag tcgaaggtcg
    11881 ccggacagat cacctccggc gtggtgtatc gattcctgca tcgcccggtc tccgcaccac
    11941 gcgtgcggct gttcgtcgac gccgccctgc gccgccacga agtggaaaac gtcgagcgca
    12001 cgctcgagca gacgcgcccg gacttttcac gcatggagaa ggcggccaag gccaggacca
    12061 gtgggccgag cccgatccgc gccgtactgc ccggcctcat cggcgcggcg atcatcattg
    12121 ccggcgccgg cttctggttc gcgcgttcct ccggtgacgt ccagaacgcc ccgcctgcgc
    12181 ctgccgcgca ggtagttagc ccagaggccg aaccgcccgc ggccgaaccc gcgccggccg
    12241 tcgccagcgt tcccgagcag aagaccgtcg agttgccccc gcccgcagat ccggaacccg
    12301 cgcccgtggc tgccgcaccg gcagcaccac cggaagaaga cgtcgccgag cgtttgcgcc
    12361 cggctgcctc accaccagcc gtggtccggg cacccgaaga agttgcgccg cgcacaccac
    12421 ccccgccggt cctcacgttc gaggaacgcc tggctcagca actggcgcag gccgaatccg
    12481 ccctgcagcg cggcgagctc gcctcgccgc ccggtcgcag cgccgtggac ctgttccgcg
    12541 gtgcactgga gctcgatccg agcaactcgc tcgccaaggg tggcctgatc cgggttgccg
    12601 acagattgct gaccgccgcc gagcgaacgc tgaccgcggg caatgtggag gacgcgcgaa
    12661 gaatggtggc ggttgccgag tcgctgaccc ccgccaccgc acgcggtgct ttcctgacga
    12721 tgcagatcga gcgcgaacac gaacgcgaag cgctgcagcg cgcgaaagac agcagcaccc
    12781 aggacaagct ggaaaaaggc gccacgtatt tgcggctcgc gaatgcgcga ctgcgcagtg
    12841 gtgcgctgat cgaaccttcc gaagacaatg cgcgctttta cctggaggcc gcgagccagc
    12901 tcctgcccga cgacgacgcg gggctcgcgg agacccggcg agggctgcag agggcgctgc
    12961 tcgatcgcgc ctcggccgcc gccagcgacg gcaacgccgc ggagaccgag cgctggctgg
    13021 cgaacgccga tgacgctggc gcgccacgcg cggatatcgc cgccattcgc cgctcgctca
    13081 aggacacgga aatcagcgca cgtgccgcga agatgacgac gctcacccag ttgttcacca
    13141 gcgccctggc ggcgaaccgg ttgctgcagc cgccgagtga caacgccaag actcatctgc
    13201 ttggcatgat cgacctcgat ggcaaccatc cggcagtggc cagtgcgcgc cagggtctcg
    13261 gcaacgcgct tcttggcgaa ttccgcggcg caatcgcgcg cggcgatctc accgcggccg
    13321 acacctggct gaacgaagca cgcaccgtcg gcttcaccgg aaatgaactc agggccgcgg
    13381 aaggtgatct cgccggtgcg cgcgggaaag caaattcggg cagcagcgta gtcggcgaga
    13441 gagcgctgga gcgcatcgaa tacgtggcgc cgaaattccc gctcgtggcg cgcaatcgcg
    13501 gcagtaccgg cagtgccggc agtaccggct gggtcgagct ggaattcacg gtgttgcccg
    13561 atggctcgac cggaaacatc gtcgtcacca attcgaaccc tcgccggacc tttgacgcct
    13621 ccgccgttac ggcagttggc cagtggcgct acaaaccgct gattcgcgac ggcaagcccg
    13681 tcgagcagcg cgtcgccgtt cgaatcagat tcgcggatca atagatggac gcgcctcgca
    13741 cacaacgatt cctgatcgtc gagaacgacg ccaagtacgc ggcgtggttg cagcacacgg
    13801 tgagcgccgg ctggcccaac gattccatcg tcatcatgga ctggacctcg ttcggccgcg
    13861 tgcgcaccgc gatgaccgtg cgggactacg acatcgtgat gctcgcgctg gtcttcgatg
    13921 aggggatcga ggaaccgacc accgacagct tcgactggat ccgcaagctg cgcagtcagc
    13981 ccggctttcc cgagatggtc attctcgccg aaggcggcag cgagctggcc gccatccgca
    14041 cgctgcgact cggcgccgca gactatctac ccaagcgact gctgacgccg cctcgtctgc
    14101 agcgttccct caagctgacg ctgcgcgcca ttgaaaagga agcccagcgg cgcgccgagc
    14161 gcagcgcgca aactgcggcc aaagccgaga cggacaaaca cgccgaaagc ctcacgggct
    14221 cggtgaaggt cgcaccgccc accgcttcac cgcccgcagc cacgccttca cacatctcga
    14281 cccataccgg cgcgagcctg gcgaaagccg ccgcgcaggc cgccgcgcag acaagcgtgc
    14341 cgccgaaacc cgcagccgcg cagatgccgc ccgtcgcacc ggcgccgccg cgcgcgcata
    14401 tcgccacgca caccggcgcc agcctcgccg tggcggccaa ggccgccctg gccgcgcatg
    14461 cgccgcccaa gctcgagcct tccaacaagg aagcgccgaa ggaaacgaag cccatcgcgc
    14521 cggaaagcca ccagattccc ggctacacca ttcttcagaa gatcggcgag tccgaggccg
    14581 ctgccgtgta cctggcgatc gccgaagacc tcggccacaa cgtggcgctg aagatctcca
    14641 agcgcaaaca ctccggcaac gtcgacacgg ccgcctccga caccggtcag cgctccatca
    14701 tgttccagcg cgaattcgag gccatcgccg cgctcgacca tccatccatc atcgacctgt
    14761 tcgactacgg cattcacgaa ggcatcgaat acctggcgat ggaatatttt ccgtgcggcg
    14821 acctcaaggc gcgactgcag aacccgctga cgtccgacga agccatcgcc ttcctcaggg
    14881 aaatcgcgcg ctccctcaag gtggtgcacg atgcaggcat catccatcgt gacctcaagc
    14941 cgccgaacgt catgctgcgc gacaacggca acgtcgtgct catcgacttc ggcctggcgc
    15001 gcagcctgtt gtccggcgac ggcagcacgc gtaccggcgt gctgcgtggc tctccttact
    15061 atatgagtcc cgaacaggcg cagggcgagg cgctcgacgc gcgcaccgac ctgtacagcc
    15121 tcggcgtcat cctctacgaa atgctcgcgg gcaagaaacc gtacctcggc gcctcggcaa
    15181 ttgatgtgct gcaacaacac gtgatggcgc cggtcccgga actgccggtg catcacctcg
    15241 gctaccaacc tttgctggaa cgactgatgt cgaaatctcg cgagcagcgc attgcctcct
    15301 gcgatgaact gctggccgcg ctcgaacaga tgtcgcatgc gatgagcgat agcggcatca
    15361 tcgatccgga aaccctggtc tcggccggag ccgggagctg aggaggccga atggaattca
    15421 gcacctttcc catcgtcgcg ctagccatgg cgctgatctg cctgatcggc gtcaccatct
    15481 atgccatcac gcagcgtggc agcctccgtt cgcgtgacga gcagctggtc gtggcgcgtg
    15541 cccaggccta cgccatgctc gagctgtcgc aggccggcgt attgtttctc gatcgcgaac
    15601 acaagctcat gggcgaggcc tcggccgccg cgccggagtt gttaggacat acctgcggcg
    15661 caggcaccgc gttcgtgcag gtcatcgccg acctggtcga cgtccaggtg cggcgccagg
    15721 cagtcgcctt tctcgaatcc atgtggaagg ccgagcccgc ctcggtagtc gatgccacgc
    15781 agaatccact cgccaaagtt cattccggcg cccgtcacct ggcgatccgc ttcgcgcgcc
    15841 tggtggtcga cggccgtgtc catcacatca tcgtttccat cgagcgcatc tccgcgccgc
    15901 gcctgatgcc gctcaccatc gaagtgccgg cgctcaatga agaaacgttc gcgaaacggc
    15961 tcgcgggtga aaccggcacg cggccggcgc tcaacatcgc atcgatggct gcgaacaccg
    16021 agctcgtcac cgccgcaccg gaatcagctg tggccgcggc tccgcctgct cccgctgctc
    16081 ctgcggctcc cgcgcctgtt ccgtccgccg cgcacgccag cccgccgccg gttgcgccga
    16141 tgtcgaccgg tgaattcgaa ctcggaccga tggacgtcac cgaagattca attccgtcgt
    16201 tgcccgtgct cagcgatgcg gccgccgccg ccatggcaaa gctcgcgccg gcaagcgaga
    16261 ccgtcgacaa gacccagaag atcgcgacac tctcggcggc atcactgcct tccaatgtgc
    16321 ccgcgtccga tgcggtcacc gcgaccatgc gccgcctgga cgaggaagaa aaacagctcg
    16381 cgaccaccat cacgtcgccg gacgtcgcgc aggaagcttc taaaccgaag cccacgacgc
    16441 ccgagctcga cccgtccggc atcatcgacc cgccagatcc gcgcttgagc gaagtgctca
    16501 gggaagtcat gcacatcgat gccggccggc tcgagacttt cctggccgag gctcgcgaga
    16561 aagccgggca gctgcgcgcc atcatgaagc tccccgcgcg cgaaccgcag gccttccgcg
    16621 agaaactggt tttgatcctc gagctgatcc gcggcattca tgtgcgcgcc gaacgcattc
    16681 cagtgccgtc ggtgtgtgaa cgcgctgatc ggttcgcagc tgcgctcaat gccttgcgtg
    16741 acaagcagac gttatccggc aacgacttcc tgccactcgc ggtgaagctc gacgatctgc
    16801 tgagccatct ggccatcctg ggtgaggtgg tgacgcgcct gcgcgagtgg cgcgttcaga
    16861 atggcgagag cggcgcgcca tcgatcgacg ccacgcagcg gtcggcgacg gccacgggca
    16921 ccaccatccg ccaacctctg atgcgtgacg ccgctccttc agccaccggc atcacgaaag
    16981 ccgcccggct ctcggacctt tcgcaggaaa gcctcgacga aatggccctc ttcctcgccg
    17041 acatgtattc caagcgcgtc tcgctcgtgg tcgtgggact cgaagatgtg cccacccact
    17101 accggcgctc agtcgaaaag attctcggtc agctcatcca caacgcgatc cgccatggcg
    17161 tcgagaaccc ggcggatcgc gcggtgctcg acaagccgga agtcggaacc atcgccgtgc
    17221 agttcgcgcg catgggctcg gacggttacc agctcagcgt ccaggacgac ggccgcggac
    17281 tcgatcacga caagatccgc gcggaggccg ttcgccaggg cgtgctgagc gccgaggtcg
    17341 ccgccaatat cgacacgcgc aaactcgcgg gcctcatttt ccgccccggt ttcaccaccg
    17401 tcggcgaagg cggcgcgcgc ggcatcggca tggacgtggt gcgcgatctc gtcaccaagg
    17461 ctgggggacg cgtcggcatc gcgacgaagc ccggcgaata cacccgcttc cgcatttcgc
    17521 tgccgcacga gaagaaggcc tccgacgccg cagtcgcgta attcttcagc tctcgcgggt
    17581 gtggcggaat tggttgcgga tatgcgcgtt gaaaaactcg cctttcgaga acgcgcggct
    17641 catcgccgaa taagtttctt ccgggacgtc gtgataccga tagcgccggc ccgagacgaa
    17701 cacgagctcc agccgtttct gctgcgggtc atatcgatac gaacggatga cgctcgaggg
    17761 catgttcgag cgaccggtgc cgcatagcgg agtttccgct ccgcgcacgg tggtcaactc
    17821 gtctgatgct tcgtgcggat tcctccgacg tcaggcgacc gacttctgct ctttttcctt
    17881 gtcgaagcgg cggcccagcc accacccgtt gacggcccag gcggcgcaca cgccggcacc
    17941 taacaaggca gcctgcgcgg gattcatgcc gccggccttg agcccgtcct gcacctgtgc
    18001 gaccacggca tccgagaagc gatatacggt cacgtcgacg aaattcttcg ctttgtactt
    18061 cgtttccgtg tcgagccggc tccagagcat ttcacggccc ggccgaatga aggcgtactc
    18121 accccagcgg cgcaggacca tgaccgtggc gagcaccagg aaggtgccca tgctcgccag
    18181 tatcaggaag ccacccatga tgagcagcgg cagaatgacc agcagcgcgg tgacacccag
    18241 cttttgcgcc agccgccccg tgatgagcac ctgcgatatg actgtcaacg tctgaacgat
    18301 gtaatcgatg cgcgcgaaca tctgggttcg ctgctcgggg tcgtcgaaca tgtccttcac
    18361 gagccgcagc tgctcgaagt agaggatcgt gttcgccgtg gagatcatga tgacgaacag
    18421 cgcgataccc atgaggtacg gcgacttgag cacgatggac acgccggcaa acggattgcc
    18481 gccgacgccc cgctgcgctt gtgtcgcccg gcccgccgcg gcgtcggcgg cggccgccag
    18541 gtccgcgtga tggcgcatct cggcgagcag tgcgcgctga cagaagatgg cgatagaaaa
    18601 catcgcggca ccgaagaaca ggatgccgga aatccccacg tggatgacga tgcgatcggt
    18661 caggaaaggg ccgacgagcg cgccggtggt gccgcccgcg gcgatgaaac cgaacagccg
    18721 cttggtctgc tcgctcctga acaactcgag caggaaactc cagaaaaccg aaatcaggaa
    18781 gagattgatg acgctgatgc cgacgtagaa cacgcgccca acgatcatgt tgttcggatc
    18841 ggcgcgaaac accaggccgg tggcagccaa tgccactgcc acgcttccat agatggcagg
    18901 caccagggta gcgcggctca cgcgcgcgac caggaatcca taaaccggga tgatcgcgat
    18961 ggcgaacacc gccgtccaga tccaggtgtc gctggtgatg gcttcgccaa ggatggtgcc
    19021 gaccgtctcg cgaatcggac gtactgaaaa gtacccgccc atgacgcaga acatgagagc
    19081 gaacgcggtg aacaccgcgc gcttctcgcc gggctcgatg cggacgaagc gtgcgaggaa
    19141 atcgtcgaac ttttgactca tgcgtgaagc ttcttcggcc ggtttgttag cgcccgattc
    19201 taggcattag tccaccgaca gggcgggtaa attcgccagg tcgatcgtca gacccggccg
    19261 gctgacggtg gccactgcac cgtgctcgcg cagcttgacg gccagcgctt cggccgaggg
    19321 cacttcacca tgcacgagat ataccggagg gcggcccgcg aagctgtcat accaacgtag
    19381 cagatcgtcc tggtcaccat gcgccgagag gccgccgagc gtatggacct gcgcgagaca
    19441 acgcacacgc tggccgtgaa tgcgcacttc cggctgccgg tcgacgagct gtcgacccag
    19501 cgtacccggt gcctggaagc caacgatgac cacgtggcac tcgggccgct ccaggttgtg
    19561 tttgaggtga tggagcacac ggccgccatt ggccattccg gagcccgcga tgatcaacgc
    19621 gccgctgcgc acatggttga tcgcgcgcga atcctcggac gattccgaca atacgaggtt
    19681 cggcagcggc ggcatgccgc gaaagttctc acgcagccgc gtggtttctt cgtcgaaacg
    19741 ttccgggttg ttccagtaca cccggctggc ggcgatggcc atgggactgt cgagaaagac
    19801 cttccaccgc gccaggtgcc actcggcgta atgcttcgcc agtagataga gcaactcctg
    19861 gctccggccg atggcgaacg ccggcacgat gacattgccg ccatcgcgct ccgcctgcgc
    19921 gagaatcccg ccaagctcac gctccgtgtc ttcacggccg cgatgcagcc gattgccata
    19981 ggtgctctcc atgagcacga gatcggcgga ttcgaacttg taagggtcct gcaggatggg
    20041 cgtgtcgtat tgcccaaggt cgcccgagaa cacgagcttg cggcgggttt cgccctcact
    20101 cgcccacaac tccacgctgg acgaaccgag aatgtgcccc gcatcgcgca cccgcacgac
    20161 gaccccggac agcagcccgc gctgcgtgtc gtaaggcatc ggaacgatct gccgcagcgt
    20221 cgtctcgaca tcatcgaggt cgtacagcgg ctcgatgcgt tccccgtgcc gtgctgcgcg
    20281 ctcggagtcg cgcagcgcca ggctcgccga atcccgcagc aggatgggca ccaggtcggc
    20341 gcaggccttg ttggtgtaga taggcccgcg ataaccgcgc ttgacgagca gcggcaggcg
    20401 cccgcagtga tcgatgtgcg catgactcag gacgacggca tcgatagccg cggcttcgaa
    20461 gggaaacggc agccggttgc gggccggcgc atcgtggccg ccctggatca tgccgcagtc
    20521 cagcaacagg tcccggcccg ccaccctcag gatgtggcag gaaccggtca cttcgcccgc
    20581 agcgccgaaa aattccaatt gcatgcgggg cataccttaa catcgccgca tcccgaacat
    20641 tccggagcgc gccatggaca ccaagtcatc ctcgggtttc gaccgtcgca ctttcctgac
    20701 ggccgcggtt gccaccggcg tcgtcggcgg tggactggtt ctgggcatcc ggtatgcgaa
    20761 tcggcgcgag gaagcggcag gtgctgccgc atccggtcgc acgctggctc cgaatgcctt
    20821 cgtgcgcgtg gcgccggacg acaccatcac cgtggtgatc ggcaaggcgg agatgggcca
    20881 gggcatctac accggccttg ccatggcgct ggcggaagag ctcgacgtgg atccgaagcg
    20941 cgtggtcgtg gagcttgccg gcgcggatcc cgcattcaac gtgccgggca tgcccatcca
    21001 gatcaccggc ggcagcatga gcaccagcac cacctacacg cagctgcgcg aagcgggcgc
    21061 ccgcgcgcgc gccatgttgt tagccgccgc ggcgcagaag tggaatgtgg acgccgccga
    21121 actcacaacc tttgacggca aggtcaatcg cggctcgaag agcctcacgt atggctcact
    21181 ggccgatgcc gcctcgcaac ttcccgttcc cgagaaggtg acgctcaagg atccggcgca
    21241 gttccgttac ctcggcaagc cgatcaagcg gctcgacgcg cccatgaaag tggacggtag
    21301 cgccaagttc ggcctcgaca tgcgcgtacc gggcatggtg ttcgcggtca ttgcgcgccc
    21361 gcccgtaatc ggcgcgacgc tcgggaacgt ggatgacagc gccgcgcgcg ccgtttcggg
    21421 cgttgtcgac gtgaaaacga ttcccgccgg cgtggcggtc tacgccacga acacctgggc
    21481 agcaaagcgc ggccgcgacg cgctcaagct cgactggaac gaaggaccag aggcgaacta
    21541 ctccaccacc gcgcaacgcc gcgaatatca gcgcctgctc ggcaagcccg gtgccgtcgc
    21601 gcgaatcagc ggcgatgtca aaaccgcgtt gagctcagcc gccaagcgca tggacgtcga
    21661 atacgagctg ccttatctgg ctcactcgcc aatggaaccg ctcaattgcc tggccgacgt
    21721 gcgtagcgac ggatgcgacc tgtggctggg cacgcagatg caaacgacag atcgcgatgc
    21781 cgcggcccgt gagctcggac tcgagccgtc caaagtgacg ctgcacaaca tgtttctcgg
    21841 tggcggcttc gggcggcgcg cacagcgcaa taacgaagtg gtcatcgaag ccgttcagtt
    21901 gtcgaaggcg atcggcaaac ccgtattgag tgtgtacacg cgcgaagacg acgtgcgtgg
    21961 tctctcctat cgcccgcacg tgatgtcacg cgtgcgtggc ggtgtcgatg ccgcgggaat
    22021 gcccgtggcc tggcagcaga ccatcgtgag ccagggtgtg ctgcgcggcg gctggaccga
    22081 cgccttcatt ccgaagggtc aggcatacga tcagtcgagc gtcgaaggcg cctcggacat
    22141 gccgtatgac attcccaatg tgcaggtgga cacgcacgaa ggcaccagca aagtgcccgt
    22201 gttgtggtgg cgctcggtgg gccattcaca caccgggttc accgtgaact gcgcgctcga
    22261 tgagctcgcg gtgcttgggg gccaggaccc ggtcgagttg cgccgcaagt tgttagccgg
    22321 gaaaccgcgt gtgctggcgg tgctgaatgc agtggcggag tcgagcaact gggccggcgg
    22381 cgcgcccgcg ggtcgtggcc ggggcgtggc actgcaggag tctttcggca gcatcgtcgc
    22441 cgaggtcgcg gaggtgtcgg tggaagacgg cgccgtacgc gtacacaagg tgtggtgcgc
    22501 catcgattgc ggcttcgccg tgcatcccga gggcgtcaag gcgcaaatgg agagtgccat
    22561 caactacggc ctcacggccg cgttgtacgg cgagatcacc ttcaccggcg gcaaggtgga
    22621 ccagagcaac ttccacgact accagatcct gcgcatcaac gaggcgccgg agatccatgt
    22681 gaccatcgtg aacagcggtg agcagatggg cggcgccggc gagcccggaa ctccgccg
//
LOCUS       DASQJS010000364        10587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3054233, whole genome shotgun sequence.
ACCESSION   DASQJS010000364 DASQJS010000000
VERSION     DASQJS010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10587
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3054233"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VEW08_14420"
     CDS             complement(<1..467)
                     /locus_tag="VEW08_14420"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HYJ41984.1"
                     /translation="MWNKPEVAQELGKERARLTADLVDMDKATGAVTSAVELLDLAES
                     ENDEGMAAEIERDAGKIETDVRKLEFKRMFRGEMDSHNAFLDIQAGAGGTEAQDWAQM
                     LLRMYLKWAASRGFDAEVIDSQAGEVAGMKSATIEIKGDYAYGWLRTETGVHRL"
     gene            complement(613..1077)
                     /locus_tag="VEW08_14425"
     CDS             complement(613..1077)
                     /locus_tag="VEW08_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ41985.1"
                     /translation="MTTGTYTIREGDPLDARVRALIAELDAFNSALYPAESNHFDAPE
                     TLAEARAIFLVVEQDGELVGCGAAKNCGGWAELKRMYLKPKARGGGMALAMLVKLLDW
                     ARAEKLPLARLETGNVSVGALKLYRRAGFKEIPAFPPYKPDPLSIFMERALR"
     gene            complement(1074..2786)
                     /gene="recJ"
                     /locus_tag="VEW08_14430"
     CDS             complement(1074..2786)
                     /gene="recJ"
                     /locus_tag="VEW08_14430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HYJ41986.1"
                     /translation="MELVIERRAARGNEITDLALHPVLRRAYAARGVCHSQELELTLD
                     RLMPVGSLDSVETAAALILQHLERRILVVGDFDADGATSTALIVRCLRAWGFASVDFL
                     VPNRFEFGYGLTPEIVGLAAERNPSLIVTVDNGISSNAGVAAARARGIQVLVTDHHLP
                     GASLPDADVIVNPNVPGSTFGSRALAGVGVAFYVMAAVRREIDARGTVVMPPVTDFLD
                     LVALGTVADLVPLDVNNRVLVAQGIRRIRAGRAVPGIRALLEIGKRPIAALTAADLGF
                     TIGPRLNAAGRLDDMSIGIRCLLADNEAEAMALATRLDQLNVERREIEARMQGVALAA
                     VKSLRDPAANAPARHGVCLFDADWHQGVVGLVASRIKDRVRRPVIAFARNADGTLRGS
                     ARSVPGIHIRDVLDGIATRHPELINRFGGHAMAAGLSLEEKHLDAFARAFDEEVARWR
                     DPSIPSNRVETDGELTSDEIALDTAQALRDGGPWGQAFPEPSFDGRFAIKNARVVGDK
                     HLKMWVTTGDQSRNFDAIAFNFKGSDENTALPEGDVQLVYRLDINEYKGERRLQLLVD
                     HVLP"
     gene            complement(2794..3807)
                     /locus_tag="VEW08_14435"
     CDS             complement(2794..3807)
                     /locus_tag="VEW08_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ41987.1"
                     /translation="MRRLSLILSDLYLPSEAAGRGAGLPAALDLPHLDWLLRFAGRVD
                     RVRDWRSWLSADLGRVDLARMTVAQACAQGLPDSLSSNAWLATPVHLEARIDHVRLAD
                     RGLLRLTADERDSWRQDFTRAFGPEYALHDSGERGFLLSGASASRVASVDPARLLDAD
                     IGRALPTGPSAGELRRLGTEIEMWLHGAPGNTARELQRQRRISAFWLWGGGESMPVNV
                     NTSPHAAPRFFGGDAYLSALAGSPVPAPPVSFAALDAGESRCVVELAPMSGTKGESLA
                     ALDSNWFGPAREALNQGGLDSLDLIANDRWFRISTGSGWRIWRRRSSWLDQLARHLPE
                     AKA"
     gene            3828..4217
                     /locus_tag="VEW08_14440"
     CDS             3828..4217
                     /locus_tag="VEW08_14440"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HYJ41988.1"
                     /translation="MKFTQQRPDGINLIRRYGADFIAIGESEIRESCIVSASSLSPWA
                     PRSVGELTPEHFLPLFALEPEVVVLSTGVRQVFPRAFLRAEFATRKIGLEVMEIGAAC
                     RTYNVLVSEERKVLVAILLPGPSKGPE"
     gene            complement(4559..5194)
                     /locus_tag="VEW08_14445"
     CDS             complement(4559..5194)
                     /locus_tag="VEW08_14445"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HYJ41989.1"
                     /translation="MTSARTRDRLVQRLRDQGIANPAVLDRIRNVPRHIFVDEALASR
                     AYEDTALPIGFRQTISQPYIVARMTEALVEGGRKIGKVLEVGTGCGYQTAVLAPFVKK
                     IYSIERIAPLAKRAKATLRELGIKNVMLKHGDGTTGWKSQKPFDGILVAAAPLAVPEA
                     LFAQLANKGRLIVPVGPEGSQQLIRYTRRDEHLEREVLGAVAFVPMLGGTA"
     gene            complement(5221..5994)
                     /gene="surE"
                     /locus_tag="VEW08_14450"
     CDS             complement(5221..5994)
                     /gene="surE"
                     /locus_tag="VEW08_14450"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HYJ41990.1"
                     /translation="MRILISNDDGYRAAGIKALREGLKGLGDLTVVAPDRNRSGASNS
                     LTLELPLRVGQAEADVYFVEGTPTDCVHLAVSGLFPFEPDMVVSGVNDGSNLGDDVLY
                     SGTVAAATEGRFLGKAAVAVSLHTDGLRGDVTRYFETGAHYARVIVERLSATPLTLGG
                     RATILNVNVPNLPVSAVKGIKVTRLGNRHRSEAVIKAEDPRGRPVYWVGPAGASQDAG
                     PGTDFHAIAEGYVSVTPLTVDLTNHAALDGLRSWFGPDA"
     gene            6034..6591
                     /locus_tag="VEW08_14455"
     CDS             6034..6591
                     /locus_tag="VEW08_14455"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HYJ41991.1"
                     /translation="MNGKRTQRDEKADAEVFRDAMRGVKPIATRTRVPPAKRKPPPKA
                     RFTAADRAMVLVESLQGPGLDEITDTGDEISFRRNGVQDDVMRKLKRGEYRVEEVCDL
                     HGLRVEEAKAALREFLADALAHNLRCVRIIHGKGMNSGPRGPVIKTAVNMILRKTAPV
                     LAFTSARRVDGGTGAINVLLSKGAG"
     gene            complement(6655..7437)
                     /locus_tag="VEW08_14460"
     CDS             complement(6655..7437)
                     /locus_tag="VEW08_14460"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYJ41992.1"
                     /translation="MLEISETYVAEGSPDSGRIRMIKLARPPVNALNGELVRKLIEAI
                     GAAKDDAAIVVTGQPGIFSGGLDVPGMLGLDREGIAALFVDLWRLQRAIAASPAPVVF
                     GLTGHCPAGGTVLAIHGDYRVMARGEFRIGLNEVQVGLFPGGVIHGAFKRLVGGHAAQ
                     LLTRGALIDPATALRMGLVDELCDAEQVPGRVLEVAREICTLPREPMLRTRALVRRDL
                     VDLFGNPGHALIQEREFSAMGVDMWFVPATQERLKAMFLKKR"
     gene            complement(7440..8528)
                     /locus_tag="VEW08_14465"
     CDS             complement(7440..8528)
                     /locus_tag="VEW08_14465"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HYJ41993.1"
                     /translation="MIPDHWRRAALAPPAAWGAPVGSGVLRTTPEDFEVDEILGFSAG
                     GEGPHALLHVRKCGANTEWVARELARAAGVKPFEVGFAGLKDRNAVTTQHFTVPRGKR
                     AAEEFTGLKGEGYEVLSAAPHQRKLPRGALEGNRFVITVRGLACDPAVLNQRLQSIAS
                     GGAPNYFGEQRFGREAGNLGQVLRTAQQLAQKPEEGGRSGRRERRGRDDAGFMLSAAR
                     SVIFNGILAERVVRGTWNRLGTGDVANLDGRGSVFAVAQVDPELEQRCTALDVHPTAP
                     LAGAGQSLATGEVLALEEAVSAQFPEALSVISAEGMKSERRALRIRVRNLEHEYSGDT
                     LRLRFALSAGSFATTVLREIIASAATGE"
     gene            complement(8525..8992)
                     /gene="ispF"
                     /locus_tag="VEW08_14470"
     CDS             complement(8525..8992)
                     /gene="ispF"
                     /locus_tag="VEW08_14470"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HYJ41994.1"
                     /translation="MRIGSGFDVHAFGPGDSIVLGGVRIPHTRGVVAHSDGDVVLHAL
                     CDALLGAAGLGDIGMHFPDTDPVWKGADSRRFVAAVLDMLSVRKLRISNADITLLAQT
                     PKVSPYRLEIRRSLAQMLGVTENQVNIKATTTEHLGFIGRNEGLAAQAIVLLE"
     gene            complement(8992..9708)
                     /gene="ispD"
                     /locus_tag="VEW08_14475"
     CDS             complement(8992..9708)
                     /gene="ispD"
                     /locus_tag="VEW08_14475"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HYJ41995.1"
                     /translation="MRYFVVIPAAGSGRRFSASVPKQYAALGSSTVIEHALSAFESDP
                     DCAGIVVAVAADDSLWPVIAARRSRLIETAEGGEERAHTVRNALRALSARVRPDDWIM
                     VHDAARPCLSVSDLQQLKRELASHPVGGLLAVPLADTLKRALEPGAHSTHVDSTIDRN
                     GLWRAVTPQVFRCGVLLRAIEAALEAGKTPTDEAQAIEWSGQRPMLIAGRADNIKVTT
                     ADDLALAAAILSVRAAQEIK"
     gene            complement(9725..10054)
                     /gene="ftsB"
                     /locus_tag="VEW08_14480"
     CDS             complement(9725..10054)
                     /gene="ftsB"
                     /locus_tag="VEW08_14480"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HYJ41996.1"
                     /translation="MKWLTGVLLTVVLLLQYRVWLSGDGVRELTRLSEAVERQKSENE
                     EAVERNQQLIAEVADLKAGMAAIEERARSELGMIGRNETFYQVVPVRPRTALPTRPAE
                     QTKTAAR"
     gene            10103..10333
                     /locus_tag="VEW08_14485"
     CDS             10103..10333
                     /locus_tag="VEW08_14485"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HYJ41997.1"
                     /translation="MKTAKINIHEAKTHLSRLIEDVAGGSEILIAKGGRAMARLVPLR
                     RDDTPRRPGLLKGKLRIADDFERPWTEGIAPA"
     gene            10362..>10587
                     /locus_tag="VEW08_14490"
     CDS             10362..>10587
                     /locus_tag="VEW08_14490"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYJ41998.1"
                     /translation="MRLLLDTQIYLWWLADSRKLAKATRERIEAADEVYVSAASIVEC
                     EQKITSGLLEADADELARGIRASGFTELPVR"
BASE COUNT         1875 a         3503 c         3382 g         1827 t
ORIGIN      
        1 agccggtgca cgcccgtctc ggtacgcagc cagccgtacg cgtagtcgcc cttgatctcg
       61 atggtggcgc tcttcatgcc cgcgacttca ccggcctgtg agtcgatgac ttcggcatcg
      121 aagccgcgcg acgccgccca cttgagatac atgcgcagca gcatctgcgc ccagtcctgg
      181 gcctcggtgc cgcccgcgcc cgcctggatg tcgaggaagg cgttgtgtga atccatctcg
      241 ccgcggaaca tgcgcttgaa ctcgagcttg cgcacgtcgg tttcgatttt gcccgcgtcg
      301 cgttcgatct cggcggccat gccttcgtcg ttctcgctct cggcgagatc gagtagctcg
      361 acggcgctgg tgacggcgcc cgtggccttg tccatgtcga ccaggtccgc cgtgaggcgt
      421 gcgcgttcct tgcccagttc ctgggcaacc tcgggtttgt tccagattgc ggggtcttcg
      481 agctcgcggc ttacttcttc gagccgctct ttcttttggt cgtactcaaa gaaaccccct
      541 caaggaagac atacgttcct cgagatcttt gagctgacgt ttcagcatgt tgatttccat
      601 agggcgcgga gtctatctga gcgcgcgctc catgaatatc gacaacgggt cgggtttgta
      661 gggtggaaac gcaggaattt ccttgaaacc cgcgcgccgg taaagcttga gcgcgccgac
      721 gctgacattg ccggtttcga ggcgcgccag aggcagcttt tccgcgcgtg cccagtcgag
      781 cagcttgacg agcatcgcaa gcgccatgcc accgccgcgc gccttgggct tgaggtacat
      841 gcgcttcaat tctgcccagc cgccgcaatt cttcgccgcg ccgcaaccga cgagttcacc
      901 gtcctgttca accaccagga atatggctcg cgcttccgcg agggtctccg gcgcatcgaa
      961 atgattgctc tccgccggat acagcgcgga gttgaaggca tccagctccg caatgagcgc
     1021 gcgtacgcgc gcgtcgagag gatcaccttc acgaatcgtg tacgtaccgg tggtcatggc
     1081 aacacgtggt cgacgagcag ctgcagacgg cgctcgccct tgtattcgtt gatgtcgagc
     1141 cggtagacca gctgcacatc gccttcgggc aacgccgtgt tctcgtcgct gcctttgaaa
     1201 ttgaacgcga tggcgtcgaa gttgcgcgac tgatcgcccg tggtgaccca catcttcaga
     1261 tgtttgtcgc ccaccacgcg tgcgttcttg atggcgaacc tgccgtcaaa ggacggctcg
     1321 ggaaatgcct ggccccaggg accgccgtcg cgcagcgcct gcgcggtgtc gagcgcgatt
     1381 tcgtcgctcg tgagctcccc gtcggtttcc acgcggttcg acggaatgga cggatcgcgc
     1441 cagcgcgcca cttcttcatc gaaggcacgc gcgaaggcat cgaggtgttt ctcttccaac
     1501 gacagccccg cggccatcgc gtgaccgccg aaacggttga tgagctcagg atgccgcgtg
     1561 gcgatgccgt cgagaacatc gcgaatgtga atgccgggaa ccgagcgtgc cgagccacgc
     1621 aacgtgccat cggcattgcg tgcgaaggcg atgaccgggc gacgcacgcg atccttgatg
     1681 cgactggcca cgaggccgac gacaccctgg tgccagtcgg cgtcgaacag gcacacgccg
     1741 tgacgcgcgg gcgcattggc cgccgggtcg cgcaggctct taaccgccgc cagcgccacg
     1801 ccctgcatgc gcgcctcgat ctcgcggcgc tcaacgttca gctgatcgag tcgcgtggcg
     1861 agcgccatcg cctcggcttc gttgtccgcc agcaggcagc gaatgccgat cgacatgtca
     1921 tcgagccgac ccgccgcgtt gagtcgcggc ccgatcgtga agccaagatc cgccgcggtg
     1981 agcgcggcga tcggtcgctt gccgatttcg agcagcgcac ggatgccggg gacggcgcgt
     2041 ccggcgcgaa tgcgacgaat gccctgcgcc accagcactc gattgttcac gtcgagcggc
     2101 acgagatcgg cgaccgtgcc gagcgcgacg aggtcgagga agtccgtgac tggcggcatc
     2161 accaccgtgc cgcgcgcatc gatttcgcgg cgcacagcgg ccatcacata gaaggcgaca
     2221 cccacaccgg cgagcgcgcg cgagccgaac gtgctgcccg gaacgttggg attgacgatg
     2281 acatcggcat ccggcaatga ggctcccggc aggtgatgat cggtcaccag cacctggatg
     2341 ccgcgcgccc gcgctgccgc gaccccggcg ttgctggaaa taccgttgtc gaccgtgacg
     2401 atgagtgatg gattccgctc ggcggccagc cccacgatct cgggcgtcag cccataaccg
     2461 aattcgaaac ggttggggac caggaagtcc acggacgcga atccccaggc gcgcaggcaa
     2521 cgcacgatca aggcggtgct ggtggcgcca tcggcgtcga agtctccgac cacgagaatg
     2581 cggcgttcga gatgctgaag aataagcgcg gccgccgttt cgacactatc gagcgagccc
     2641 accggcatga gtctgtcgag cgtgagctcg agctcctggc tgtggcacac gccgcgcgcc
     2701 gcgtacgcac gtcgcagaac cggatgcagc gcgagatcgg tgatttcgtt gccgcgagcc
     2761 gcgcgtcttt cgatgaccag ttccatggcg cgattatgcc tttgcctcgg gaaggtggcg
     2821 ggcaagctgg tcgagccacg aagaacgccg gcgccagatt cgccagccgg aaccggtgga
     2881 gatgcgaaac catcggtcat tggcgatgag gtcgaggctg tcgaggcctc cctgattgag
     2941 cgcttcgcgg gccggaccga accagtttga atcgagcgcc gcaagtgact cgccctttgt
     3001 cccgctcatg ggcgcgagtt cgaccacgca tcggctttcg ccggcgtcca atgcggcgaa
     3061 tgacaccggt ggcgcaggca ccggcgagcc ggcgagcgcg gatagataag catcgccacc
     3121 gaagaagcgc ggcgctgcat gtgggctggt attgacgttc accggcattg attccccgcc
     3181 tccccacaac cagaacgcgg atatgcgccg ctggcgttgg agttcgcgag cggtattacc
     3241 gggagcgccg tgaagccaca tctcgatctc ggttccaagc cgacgaagct cacccgccga
     3301 agggcccgta ggcagtgcac ggccgatatc ggcatcgagc aaacgcgccg gatcgacgct
     3361 ggcaacgcgc gacgcggacg cgccgctcaa caggaaacca cgctcgcccg aatcatgcag
     3421 cgcgtactcc ggtccgaacg cgcgagtgaa gtcctgccgc cagctgtcgc gctcgtccgc
     3481 agtcaggcga agcaatcccc ggtcggcaag ccgcacatga tcgatgcgcg cttcgagatg
     3541 caccggcgtg gcgagccacg cattgctcga caaggaatcg ggtaatcctt gcgcgcatgc
     3601 ctgcgccacg gtcatgcgtg ccagatcgac acggccgaga tccgcgctca accacgaacg
     3661 ccagtcgcgg acgcgatcga cgcgcccggc gaatcgaagc agccagtcga ggtgtggcag
     3721 gtcgagcgcg gccggaaggc cggcgccgcg cccggctgct tccgacggaa ggtagagatc
     3781 ggagaggatc agcgagaggc ggcgcatggc ggtatcgtat cggacccatg aagttcactc
     3841 agcaacgccc cgacggcatc aacctcatca ggcgctacgg tgccgacttc atcgccattg
     3901 gcgaaagcga gattcgcgag agctgcatcg tcagcgccag ctcgctttcg ccgtgggcgc
     3961 cgcgttcggt cggtgaactc actcccgaac atttcctgcc cctgttcgcg ctcgagccgg
     4021 aggtggtggt tttgtcgacc ggcgtgcgcc aggtgtttcc gcgtgctttt ctgcgagcag
     4081 aatttgcgac gcgaaagata ggtctcgaag tgatggagat cggcgcggcc tgccgtacct
     4141 acaacgtgct ggtcagcgaa gagcggaagg tgctggtggc gattctgctt ccgggtccct
     4201 cgaagggacc cgagtgaaga cacaggggga gaaagttgtt tagcgaggcc aatggttcga
     4261 agctgacaga ctcgaattac tggcgaattt tttcttcttc tattcggctt gttctacttt
     4321 ttgccgcgac ttccccgttc tcccctgtgc cttcacccgg aacccctcgc aaactcttac
     4381 gagtgtctct gtcggcgggc ggcgacatga aaacatgtta aatcctgact gacaagcctg
     4441 cgcacttgtg ccctcacaac tctcgagaat tgcgggcaca tttccacttg tagaaacaac
     4501 aagttacagc acagaccgta tgcgagaact tactcgaata ctgcgctgca gcctggagtc
     4561 aggccgtacc gccgagcatg ggaacgaagg ccaccgcgcc taacacctcg cgttccaggt
     4621 gctcgtcgcg acgcgtgtag cgaatgagtt gctgggaccc ttcggggccc acggggacga
     4681 tgaggcgtcc cttgttggcg agctgcgcaa acagcgcctc gggaaccgcc agcggcgccg
     4741 ccgcgaccag gattccatcg aatggcttct gcgacttcca gccggtggtt ccatcaccgt
     4801 gtttgagcat cacgttcttg atgccgagct cgcggagcgt cgccttcgcg cgcttcgcca
     4861 gcggcgcaat gcgctcgatg gagtagatct tcttcacgaa gggcgcgagc accgcggtct
     4921 gatagccgca gcccgtgccg acctcgagca ctttgccgat cttgcgtccg ccttcgacca
     4981 gggcttcggt catgcgcgcg acgatgtaag gctgcgaaat ggtctggcga aatccgatgg
     5041 gcagcgcagt gtcttcatag gcgcgcgacg ccagcgcttc atcgacgaag atgtggcgcg
     5101 gcacgttgcg tatgcgatcg agcacggcgg gattggcaat gccctggtcg cgcagacgtt
     5161 gcaccagccg gtcgcgtgtg cgcgcggagg tcatgccgat gccggcctgg cgattctcgt
     5221 tcacgcgtcg ggcccgaacc agctgcgcaa tccatcgagt gccgcatggt tggtgagatc
     5281 gacggtgagc ggcgtgacgg agacgtagcc ttcggcgatg gcgtgaaaat ccgtaccggg
     5341 tcccgcatcc tggctggcgc ccgcgggccc cacccagtac accggacgtc cgcgcggatc
     5401 ctcggccttg atcaccgctt cggagcgatg gcgattgccg agtcgcgtga ccttgatgcc
     5461 cttcaccgca ctcacgggaa ggttcggaac attcacgttg aggatggtgg cacgaccgcc
     5521 gagcgtgagc ggcgtggcgc tcagtcgttc gacgatcact cgcgcgtaat gggcgccggt
     5581 ctcgaagtaa cgtgtgacgt cgccgcgcaa accgtcggtg tgcaacgaga ccgcgacggc
     5641 agccttgccg agaaagcggc cctcggtggc cgccgccacc gtacccgaat acaacacatc
     5701 gtcgccgaga ttcgagccgt cattgacgcc ggagaccacc atgtcgggtt cgaacggaaa
     5761 gagtccggag acggcgaggt gcacgcaatc tgtcggcgtg ccctccacga agtagacgtc
     5821 ggcttccgcc tgccccacac gcaatggaag ttcgagcgtg agcgagttgc tggcgccgct
     5881 gcgattccga tcgggcgcca ccactgtcaa atccccgagg cccttcaacc cctcgcgcag
     5941 cgccttgatg cccgcagcac ggtaaccgtc gtcgttggat atgaggattc gcaccggcgc
     6001 gcaatatact caatgcgtca acgcggaggt agcttgaacg gcaaacgtac ccaacgtgac
     6061 gagaaggcgg atgcggaggt gtttcgcgac gcgatgcgag gcgtcaagcc gatcgcgacc
     6121 cgcactcgcg tgccgcccgc caagcgcaag ccgccgccga aggcgcgatt caccgctgcc
     6181 gatcgcgcca tggtgctggt ggaaagcctg cagggacccg gcctcgatga gatcaccgac
     6241 accggcgatg aaatctcgtt ccgccgcaac ggcgtgcagg acgacgtcat gcgcaagctc
     6301 aagcgtggtg aataccgcgt cgaagaagtg tgcgatctgc acggcctgcg cgtcgaagag
     6361 gccaaggctg ccctgcggga attcctggcc gacgcactgg cgcacaacct tcgctgcgtg
     6421 cgcatcatcc acggcaaggg catgaattcg gggccgcgcg gaccggtcat caagaccgcg
     6481 gtgaacatga tcctgcgcaa gacggcgcca gtgctggcat tcacgtcggc gcggcgagtc
     6541 gacggcggca ccggcgccat caacgtcttg ttatcgaagg gtgccgggtg aggaaagtgg
     6601 ggagtgcggt cactccccac tttcctcacc cggcaccctt cagggacgcc ttgctcagcg
     6661 cttcttgagg aacatggcct tgaggcgttc ttgggtcgcg ggcacgaacc acatgtcgac
     6721 ccccatcgcg ctgaactcac gctcctggat cagcgcgtgg cccggattgc cgaagaggtc
     6781 gaccagatcg cgacgcacga gtgcgcgcgt gcgcagcatc ggctcgcgcg gcagagtgca
     6841 gatctcgcgc gccacttcga gcacgcgccc cgggacctgc tcggcgtcgc aaagctcatc
     6901 caccaatccc attcgcagcg ccgtcgccgg gtcgatcaac gcgccacgcg tgagcagttg
     6961 cgcggcgtga ccaccaacca gacgcttgaa cgcgccgtga atcactccgc cgggaaacaa
     7021 ccccacctgc acttcgttga gtccgattcg gaactcaccc cgcgccatga cgcgatagtc
     7081 gccatgaatc gcgagtaccg tgccaccggc cgggcagtgg ccggtgaggc caaacacgac
     7141 cggcgcgggt gacgcggcga tggcgcgctg caggcgccac aggtcgacga acaatgcggc
     7201 aatgccctcg cgatccaggc ccaacatgcc cggcacgtcg aggccgccgg agaaaatgcc
     7261 gggctgcccg gtgaccacga tcgccgcgtc gtctttcgcc gcgccgatgg cctcgatgag
     7321 tttgcgcacc agctccccgt tgagagcatt caccggcgga cgtgcgagct tgatcatccg
     7381 gatgcgcccg gaatcgggtg agccttcggc aacgtacgtt tcgctgatct cgagcatggt
     7441 cattccccag tagcggcgct cgcgatgatc tcgcgaagca cggtcgtcgc aaagcttcct
     7501 gccgacaagg cgaatcgcag ccgcaaagtg tcgcctgaat attcgtgctc gaggttccgg
     7561 acgcggattc gcaatgcgcg ccgttccgat ttcatacctt ctgcggagat gacggacagc
     7621 gcctcgggga attgcgccga gactgcctct tcgagcgcca gcacctcgcc ggtagccagc
     7681 gactgtcccg cgccagccag cggcgccgtg ggatgtacgt cgagcgcggt gcagcgttgt
     7741 tcgagttccg ggtcgacctg ggccacggca aacacgctgc cgcggccgtc gagattcgca
     7801 acgtccccgg tacccagtcg gttccaggtg ccccgcacca cgcgctccgc gaggatgccg
     7861 ttgaagatga cgctgcgcgc ggccgacagc atgaaaccgg catcatcacg acctcgccgt
     7921 tcacgccttc cgcttcgccc accctcttcc ggtttctgag ccagctgttg cgcggtgcgc
     7981 agcacctgcc ccaggttgcc cgcctcgcgt ccaaagcgct gctcgccaaa atagttaggc
     8041 gcgccgccgc tggcgatgct ctgcaggcgc tggttcagca cggcggggtc acacgccagc
     8101 ccgcgcacgg tgatcacgaa acgattgccc tcgagcgcgc cgcgcggcag tttgcgctgg
     8161 tgcggcgccg cgctgagcac ttcataacct tcgcccttca gcccggtgaa ttcctcggcc
     8221 gcgcgtttgc cgcgcggcac cgtgaagtgc tgtgtcgtca ccgcattgcg gtccttgaga
     8281 cccgcgaagc ccacctcgaa tggcttgacc ccggcggcgc gcgccagctc gcgcgcgacc
     8341 cattcggtgt ttgcgccgca tttgcgcacg tgcagcagcg catgcggccc ttcgcctccg
     8401 gccgaaaaac cgagtatttc gtccacttcg aagtcttcgg gagtagtgcg cagcacaccg
     8461 ctgccgaccg gtgcgcccca ggctgcaggc ggcgccagcg cggcgcgccg ccaatggtcg
     8521 ggaatcattc cagcaacacg atggcttgtg ccgccagccc ttcattgcga ccgatgaaac
     8581 ccagatgctc ggtggtggtg gccttgatgt tcacctggtt ttcggtgaca cccagcatct
     8641 gcgccagcga acggcggatc tcgaggcgat agggtgacac tttgggtgtc tgcgccaaca
     8701 gcgtgatgtc ggcgttgctg atacgcagct tgcgcacgct gagcatgtcg agcaccgccg
     8761 cgacgaaacg ccggctgtcc gcgcccttcc acaccggatc cgtgtcgggg aagtgcatgc
     8821 cgatgtcgcc caacccggcc gcgcctaaca acgcgtcaca cagggcatgc agtaccacgt
     8881 cgccatcgct gtgcgccacc acgccgcgcg tgtgcggaat ccgcacgccg ccgagcacga
     8941 tcgaatcgcc cgggccgaag gcgtgcacgt cgaaaccgga accgattcgc atcacttgat
     9001 ctcctgcgcc gcgcgcacgc tgagtatggc ggcggccagc gccagatcgt ccgccgtggt
     9061 caccttgata ttgtccgcgc ggccggcgat gagcatgggc cgctggccgc tccactcgat
     9121 ggcctgggct tcgtccgtcg gggtcttgcc cgcctcgagc gccgcttcga tcgcacgcaa
     9181 caacacgccg caacgaaaca cctgcggtgt cacggcacgc cagagcccgt tgcgatcgat
     9241 ggtggaatcg acatgcgtcg agtgcgcgcc cggctccagt gcacgcttga gcgtgtccgc
     9301 gagcggcacc gccagcaacc cgccgaccgg atgcgaggcc agttcgcgtt tgagctgttg
     9361 caggtccgag actgataaac atggtcgcgc ggcgtcgtgg accatgatcc agtcatccgg
     9421 gcgaactcgc gccgacagcg cacgaagcgc attacgcacc gtgtgcgcac gctcctcgcc
     9481 gccttcggcg gtctcgatga gccgcgaacg gcgagccgcg atgacgggcc acagcgagtc
     9541 gtccgctgcg accgccacca cgatgccggc acagtccggg tccgactcga acgcgctcag
     9601 cgcgtgctcg atgacggtcg aactaccaag ggccgcgtat tgctttggaa cggatgcgga
     9661 gaaccggcgg ccgctgcccg cggccgggat gacgacgaaa tagcgcatgg gcgcgtagct
     9721 tacgtcagcg ggcggcggtc ttcgtctgtt cggccgggcg cgtcggcagc gcagtgcgcg
     9781 ggcggacggg gaccacctgg tagaaggttt cgttgcggcc aatcatgccg agctcgctgc
     9841 gcgcgcgctc ttcgatggcc gccatgcccg ccttcagatc cgcgacttcg gcgatgagct
     9901 gctggttgcg ctcgaccgcc tcttcgttct cggatttctg gcgctccacc gcctccgaca
     9961 ggcgggtgag ttcgcgcacg ccatcgccgg acagccagac ccggtactgc agcagcaata
    10021 cgaccgtgag cagtacgccc gtgagccact tcatgaaaag accttcccca ttgccactag
    10081 actaaactaa actaagttca ctgtgaagac cgccaagatc aatattcacg aggcgaagac
    10141 tcacctctcg aggctcatcg aagatgtggc cggtggcagc gagatcctca tcgccaaggg
    10201 tgggcgcgcc atggcccggc tcgtcccgct gcggcgcgac gacacgccgc gccggccggg
    10261 gctcctcaag ggcaagctgc gcatcgccga cgacttcgaa cggccctgga ccgaaggcat
    10321 agccccggcc tgatccgggt gccctcccct accccgccac catgcgcctg ctgctcgata
    10381 cgcagatcta tctctggtgg ctggcggact cgcgcaagct ggccaaggcc acgcgtgagc
    10441 gcatcgaggc ggcggacgag gtctacgtca gcgccgcgtc catcgtggag tgcgagcaga
    10501 agatcacgtc cggcctgctg gaggccgacg cggacgaact ggcgcgcggc attcgcgcca
    10561 gcgggtttac ggagctgccc gtgcgcg
//
LOCUS       DASQJS010000365         3812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_231056, whole genome shotgun sequence.
ACCESSION   DASQJS010000365 DASQJS010000000
VERSION     DASQJS010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231056"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(339..1121)
                     /locus_tag="VEW08_14495"
     CDS             complement(339..1121)
                     /locus_tag="VEW08_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYJ41999.1"
                     /translation="MHRQAIGIDIGGSSIKAAVVDLVRGCLIGERHSVPSPAMSAVSE
                     MLHAMVAAVPEGTAQLAAGVTFPGVVVGGTVYSAANVAKSWIGQPLAEPAAAKLKRKV
                     VAINDADAAGLAEVHFGAARDVCGTVLVLSLGTGIGSALFHDGKLVPNTEFGHMEFDG
                     MEAEARASGRARIERKLEWGAYMTELNYVLNRMHALLWPDLIVLCGGITADHPNLCDD
                     LRVPSKIKLGALRANAGIVGAAFATTLAEGVWINSAKDAVAT"
     gene            1331..3784
                     /locus_tag="VEW08_14500"
     CDS             1331..3784
                     /locus_tag="VEW08_14500"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HYJ42000.1"
                     /translation="MTSGAILRRGSLRAAIYDRLTRSLGKEPETATPRDIYDALSLAV
                     REELSARWLATQRRVARARVKRLCYLSVEFLPGRSLLNALSSLDSELIDEARTAVASM
                     GHDFDAVIALEVDPGLGNGGLGRLAACFLDSLATLQYPATGYGIRYDYGIFTQSIDTD
                     GAQRESASTWLRLHNVWETPRSDARYIVRFGGRVVNTHDVGGVQRSRWVETQDILAVG
                     FDQLVPGNRSPTVNHLRLWSGRALQPFHIEDFNAGNYPAAVEDQIDAKNLSRVLYPDD
                     STPQGKELRFKQQYFFVSASIQDILATHLAEERALEDLPTSLAVQMNDTHPTLAIPEL
                     MRLLMDQYDMPWADAWNICRSVFGYTNHTLMPEALETWPVNFFERLLPRHLEIIYRIN
                     EEFLLEVEKRFKGDGAAKTTLSIISENHGRRVRMSHLAVVGSHKVNGVAKLHSDLMKK
                     TIFSGFAQMFPDRFTNVTNGITVRRWLKQSNPLLSRLLTEKLGSAWENDLEEIERLRW
                     AADDPRFRAQFREIKRANKIALAEEVDRRTGIDVSVDSLFDVHVKRIHEYKRQLLNIL
                     YVITRYNRIKARPDEHWVPRTVMFAGKAAPGYVLAKAIIRLINNVSTTINQDPHTLTK
                     LKCVFLPDYDVSLAQTIMPAADLSQQISTAGMEASGTGNMKLALNGALTIGTLDGANI
                     EIRDSVGADNIFIFGMTADEASQRQANGYRPREIVAANAELAATLEMIRAGAFSRGGP
                     DDARLLVDRLTADGEPFLVLADFATYCEAQDRVDALYRQPEEWSRRAVLNTVGMGPFS
                     SDRSVREYAERIWNIRSVI"
BASE COUNT          724 a         1215 c         1190 g          683 t
ORIGIN      
        1 cgtggggtta cacgcctctt ttctgacgaa cggagcggcg gcgcgtcggt gcacgatcga
       61 gcgacgggtg gccctgcatg cgatgagcga gtgggccggg atacctggcc tcgttaggca
      121 cgaagccgat ccctgcgaca tcaggactct tcgcaagctc caaatcgaac tacgccagag
      181 tgtcttcgcg tgtgtcggcg gcccgcaatc tgtggcgatg ccaccctgaa cgaagggccc
      241 ggcgaaaagc gagtctcatc gcatgcaggg ccgcccgtcg ctcgaccctc cacatacgag
      301 ccacgtcccc ttctccaagc aaaagaggaa tgtccccact aagtggcgac ggcatccttc
      361 gcgctattga tccaaacgcc ttccgccagc gtggtggcaa atgccgcacc gacgatgccc
      421 gcattggccc gcagcgcacc cagcttgatc ttgctcggca cccgcaggtc gtcgcagaga
      481 ttcggatggt cggcggtgat gccgccgcac agcacgatga gatcgggcca cagcagcgcg
      541 tgcatgcggt tcagcacgta gttgagttcg gtcatgtacg cgccccactc gagtttgcgc
      601 tcgatgcgcg cccgaccgga ggcgcgggct tcggcttcca tgccgtcgaa ttccatgtgg
      661 ccgaactcgg tgttaggcac gagtttgccg tcgtggaaca atgcgctgcc gattccggta
      721 cccaggctca ggacgagaac ggtgccacag acatcgcgcg cggcgccaaa gtgcacttcg
      781 gcgaggcctg ccgcgtcggc gtcattgatg gcgaccactt tgcgcttgag cttggcggcg
      841 gcgggttcgg cgaggggctg gccgatccac gacttggcga cgttggccgc ggagtagacg
      901 gttccaccga cgacgacgcc gggaaacgtg actccggccg cgagttgagc tgtgccttcg
      961 ggcaccgcgg cgaccatggc gtgcagcatt tcgctgaccg cggacatggc cggcgagggt
     1021 acggaatggc gttcgccgat caggcatccc ctgaccaggt caacgacggc cgccttgatc
     1081 gacgagcccc cgatatcaat accaattgct tgtcggtgca ttccgcgtta actcgaagcc
     1141 attgtggtgc accgcgaaga attcggaccc ggtcacgtag agttactgtg cccatcctcg
     1201 cggggtgcat atgataccga tacaacggct ccaacatggc ttttgcatgc attgtcgggg
     1261 cgtcaagaaa aatgtcctga aatgtctcgt aagcaagtga aaggtagttc gagtgcagaa
     1321 tgagccgacg ctgacatccg gcgcgatcct caggcgtgga tcgctgcgtg cggcgattta
     1381 cgaccgcctg acgcgctcgt tgggcaaaga gcccgaaacc gcgacgccgc gtgacatata
     1441 cgatgcgcta tcactcgccg tgcgcgagga attgtccgcc cgttggcttg cgacgcagcg
     1501 acgcgttgcc cgcgctcgcg tcaagcgcct gtgctacctg tcggtggaat ttcttccggg
     1561 ccgctccctg ctgaacgccc tgtcgagcct cgattcggaa ctgatcgacg aggcgcgcac
     1621 cgctgttgcc agcatgggac acgacttcga cgccgtgatc gcgctcgaag tggatccggg
     1681 tctgggcaac ggcggcctcg gccggctcgc ggcgtgtttc ctcgattcgc tggcgacttt
     1741 gcagtatccg gcgacgggct acggcatccg ttacgactac ggcatcttca ctcagtccat
     1801 cgacaccgat ggcgcgcaac gcgaatccgc cagcacgtgg ttgcgattgc acaacgtgtg
     1861 ggaaacgccg cgatcggacg cgcgatacat cgtgcgtttc ggcggccgcg tcgtgaatac
     1921 acacgacgtc ggcggcgtgc agcgttcacg ctgggtcgaa acccaggaca tcctcgccgt
     1981 tggcttcgat cagctggtgc ccggcaatcg cagccccacc gtgaaccacc tgcgcctgtg
     2041 gtcggggcgc gcgctgcagc cgtttcacat cgaggatttc aacgccggca actatccggc
     2101 cgcggtcgaa gaccagatcg acgcgaagaa tctgtcgcgc gtgttgtacc ccgacgactc
     2161 cacgccgcag ggcaaggagc tgcgcttcaa acagcagtac ttcttcgtca gcgccagcat
     2221 ccaggacatc ctcgccacgc acctcgccga ggagcgcgca ctggaagacc tgccgacctc
     2281 gctcgccgtg cagatgaacg atacgcatcc gacgctcgcc atcccggagc tcatgcggtt
     2341 gctgatggat cagtacgaca tgccgtgggc cgatgcatgg aacatctgcc gcagcgtttt
     2401 cggttacacc aatcacacgc tgatgcccga ggcgctcgaa acctggccgg tgaatttctt
     2461 cgagcgcctg ttgccgcggc acctcgaaat catctatcgc atcaatgaag agtttcttct
     2521 cgaggtggag aagcggttca agggcgacgg cgccgccaag accacgctgt cgatcatttc
     2581 cgagaaccac ggccggcgag tgcgcatgtc gcacctggcg gtggtcggca gccacaaggt
     2641 gaacggggtc gccaagctgc actccgacct catgaagaaa accatcttct cggggttcgc
     2701 gcagatgttt ccggaccgct tcaccaacgt gaccaacggc atcacggtgc gccgctggct
     2761 gaagcagtcg aatccgctgc tgtcacggct gctgacggag aaactgggca gcgcgtggga
     2821 gaacgacctc gaggaaatcg agcgcctgcg ctgggcggcg gacgatccgc gcttccgcgc
     2881 gcagttccgc gagatcaagc gcgccaacaa gattgcgctg gcggaagagg tggaccggcg
     2941 cactggcatc gacgtcagcg tcgactcctt gttcgacgtg cacgtgaagc gcatccacga
     3001 gtacaagcgc cagctgctca acatcctcta cgtgatcacg cgctacaacc gcatcaaggc
     3061 caggccggac gagcactggg tgccgcgcac tgtcatgttc gccggcaagg cggccccggg
     3121 ttatgtcctg gcaaaggcca tcatccgact cattaataat gtgtcgacca cgatcaacca
     3181 ggacccccac acgctgacca agctcaagtg cgtgttcctg ccggactacg acgtgtcgct
     3241 ggcgcagacc atcatgccgg ccgcggacct gtcgcagcag atctccaccg ccgggatgga
     3301 ggcctcgggc acgggcaaca tgaagctcgc gctgaacggc gcgctgacca tcggcacgct
     3361 cgatggcgcg aatatcgaaa tccgcgattc ggtgggcgcc gacaacatct tcatcttcgg
     3421 catgacggct gacgaagctt cgcaacgcca ggcgaacggc taccggccgc gcgagatcgt
     3481 cgctgccaat gccgaactgg cggcgacgct cgagatgatc cgcgccggtg cattcagtcg
     3541 cggcggtccg gatgacgcac ggctgctcgt ggaccggctg acagccgacg gtgagccgtt
     3601 cctggtgctc gcggatttcg cgacctactg tgaggcacag gaccgcgtcg atgcactata
     3661 tcggcaaccc gaggagtgga gccgtcgcgc ggtgctcaac accgtcggca tggggccgtt
     3721 ttccagcgat cgcagcgtgc gtgaatacgc ggaacgcatc tggaacatcc gatcggtgat
     3781 ttaatgggga cattcttcgt ttcctggcaa ac
//
LOCUS       DASQJS010000366         9471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3682291, whole genome shotgun sequence.
ACCESSION   DASQJS010000366 DASQJS010000000
VERSION     DASQJS010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3682291"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VEW08_14505"
     CDS             complement(<1..469)
                     /locus_tag="VEW08_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ42001.1"
                     /translation="MKYADARAVVTGGASGLGNAVARHIVAAGGRVTLLDVQEGPGQA
                     AAAELGANAFFAKCDVTSEAEVNTAMESARAAMGSINLLVNCAGVIGAGRVLGKNGPM
                     AGDFFTRVVHINLIGTFLCDKAAAAIMQANTPTADGERGVIIHTSSVAAFEGQI"
     gene            complement(536..1717)
                     /locus_tag="VEW08_14510"
     CDS             complement(536..1717)
                     /locus_tag="VEW08_14510"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HYJ42002.1"
                     /translation="MDNKQVVIAAASRTPIGAFQGVFSPVTAPQLGAAAIRGALAAAG
                     VSAGDIQEVIMGCVLPAGLGQAPARQAALGAEVPRGVPTTTINKMCGSGMKAIMMAAD
                     QIRAGDVSIVVAGGLESMTNAPYLLPKARGGMRMGHGEVLDHMFYDGLQSPFDGQLMG
                     VFAEATAQKYSFSRTDQDAFATESTQRALKAVGSGAFAGEIVPVTVKGRKGETVVDKD
                     ETPFTVDLAKIATLKPAFRKDGTVTAASSSSISDGAAAAVLMSAAEAARRGIKPLARI
                     HGYTSFAQEPEWFTTAPAGAIQKLLAHLGWKAGDVHLYEVNEAFAVVTMAAMRDVGFD
                     HARTNVNGGACALGHPIGATGARIIATLVHALRARGGGRGVASLCIGGGEATAIAVEV
                     P"
     gene            complement(1731..2375)
                     /locus_tag="VEW08_14515"
     CDS             complement(1731..2375)
                     /locus_tag="VEW08_14515"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYJ42003.1"
                     /translation="MPAPARAKRSEPASPWEARRQRGSGHALKRDAVILAAARAFRQR
                     GYHNTSLDDLAASLKVTKPTLYLYVPSKEAILFECFRAGLAQIQSTLKECESAAGPAR
                     ERLFAFIRGYAAAIVGDFGWCMLRAEDQHLGAAMSRRIKLLKAGIDKRMRALIDAGVA
                     DGSIRACDTKMTAFALAGALNWMGHWYREDATLKPGEIADRFIDVFNRGLRGRD"
     gene            2434..5889
                     /locus_tag="VEW08_14520"
     CDS             2434..5889
                     /locus_tag="VEW08_14520"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HYJ42004.1"
                     /translation="MKPPEPGKPLRFVSAASLFDGHDAAINMIRRLLQSGGAEVVHLG
                     HNRSVADIVGAAIAEDADAIAVSSYQGGHNEYFRYMVDMLRERGATHIRVVVGGGGTI
                     SPDEIAELEAYGVAKIYTPEDGRSLGLNGMIADVFARVGRRTHADQIARMPAVHDHGA
                     IAHAITLLEGGAGDDARGDTGADDLRRLLSAGIANPQRAPVIGLTGTGGAGKSSLTDE
                     LLQRFLRQFPDRNVAVVAVDPTRRRSGGALLGDRIRMNSLAASNVFMRSLATRRQHLA
                     TSAVLADVIALYRAVGFDLVVVETAGIGQSDTEIVDVADLSLYVMTAEYGAASQLEKI
                     DMLDFADMIVLNKFEKRGAEDALRDVRKQWRRNHPDRMKLPDEDLPVFPTIASRFNDP
                     GVNRLFLALCSALDEECGETGRWTSADGAPLAVAPTEFISRDPLVPSARSRYLAEIAA
                     RGREEHGNAVRRADAARRAHGLYLSLQALKDPALPEPLAAATATSGKDSALDALRTAY
                     NQALDDVGADALASLRAWPAREHAVTAPEYSYTVRGREVRGANYTTSQSHTPIPKLAP
                     PKLSGWGEILDFLKNENLPGAWPYTGGVYPYRREEEDPTRMFAGEGAPERTNRRFHYL
                     AAGHEATRLSTAFDSTTLYGEDPDMRPDIFGRTGNSGVSIATLDDMKKLYSGFDLCAP
                     STSVSMTINGPAPMILAMFMNTAIDQRVEHFLRQSNRWDAVEAVLKRAGAGTGAWPAY
                     RGELPPDHGGEGLGLLGFSGRRLVELDLLTSDEYTRLRAEALSRVRGTVQADILKEDQ
                     AQNTCIFSTEFALRMMGDVQQFFSENDVRNFYSVSISGYHIAEAGANPITQLAFTLAN
                     GFTIVEYYLARGMKIDDFAPNLSFFFSNGMDAEYAVIGRVARRIWARAMRDVYKAGPR
                     SQMLKYHIQTSGRSLHAREIQFNDIRTTLQAMYALFDNCNSLHTNAYDEALTTPTEES
                     VRRAVAIQLIINRELGLNRIQNPWSGSYAITALTDLVEEAVYKEFESLAERGGVLGAM
                     ETMYQRGKIQEESMLYETRKHDGSLPIVGVNTFLNDRGASETAVAPLFRASDEEQRAQ
                     VDAVHAFQARHAQVAPEALQRLQSVAASGGNIFAELLDTVQVCSLGQITRALYEVGGQ
                     YRRHM"
     gene            complement(5896..6552)
                     /locus_tag="VEW08_14525"
     CDS             complement(5896..6552)
                     /locus_tag="VEW08_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42005.1"
                     /translation="MELDELKASWQSLDRRVEDLIAINRRLLTETISRKVRWRLAPVL
                     AGAVLNMIIGAWFALVWGKFWSAHLATPAVAVAGIALHLASIGLIVIGVVRLVLVLRI
                     DYTQPVLKIQRSLATLQEFEARSFHAVWFACWVLVPAALVAIVMGFAGVDLWDRASGF
                     LLANLVVCLAGGLAPPLLHYWARRQHGRIAAWMDAFLLSHSIARAKAAIAEIDEFARS
                     "
     gene            complement(6539..7048)
                     /locus_tag="VEW08_14530"
     CDS             complement(6539..7048)
                     /locus_tag="VEW08_14530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ42006.1"
                     /translation="MNPEPGVEFAALLDGHRRLIFKVARTYARDTADVEDLAQEIAAQ
                     LWRAFPAYDRARLFSTWMYRIALNVAISWLRAEAPRRRRSVAYDADMHEPTTDGAADD
                     DEGRRILRGFIASQDSLDRAVLLLYLEERPYSEMSEILGVSATNLTTKVNRLKDRLRN
                     YAEKYHGTR"
     gene            complement(7120..7890)
                     /locus_tag="VEW08_14535"
     CDS             complement(7120..7890)
                     /locus_tag="VEW08_14535"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYJ42007.1"
                     /translation="MTSTSPIVRAPWPAAISVPLRFIYGIYAALLFLLVALTALLAVL
                     FLPTLKLRRGTARIAARTYFLLAGMPLRLRGTENLPAGQCVVVANHASYLDGVVMAAA
                     LPPRFAFVIKREMNEVPVAGLLLRRIGSEFVDRFNRHKGGTDARRVLRTAASGQSLVF
                     FPEGTFTPEVGLGKFHTGAFAIAARAACPVVPAVILGTRRNMPATRVLPRPGPLEVRF
                     GLPIAPTVAGPEADPALRLRDQSRQAILQELGEPDLAA"
     gene            8051..8983
                     /gene="xerD"
                     /locus_tag="VEW08_14540"
     CDS             8051..8983
                     /gene="xerD"
                     /locus_tag="VEW08_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HYJ42008.1"
                     /translation="MVKQATAQNEAPAVDTADTTVSKFLDAVWMERGLSANTLAAYRA
                     DLTALARWLAERGTPIIKTTRADLLGFIAYRVEAGARPRSTARQLSSFRRFFRYFVRE
                     GVIKDDPTAQIAMPKIGRSLPKSLTEEEVESLLSAPAIADPLGHRDRTMLEVLYATGL
                     RVSELVNLKYNQINTNQGVIRIMGKGNRERLIPLGEEAVRWLTDFVRAPRSEILLDRT
                     TDYLFPTRRGDRMTRQAFWHIIKRYARKAVIDKELSPHTLRHAFATHLLNHGADLRVV
                     QMLLGHSDLSTTQIYTHVARERMKEMHGTHHPRG"
     gene            9115..>9471
                     /locus_tag="VEW08_14545"
     CDS             9115..>9471
                     /locus_tag="VEW08_14545"
                     /inference="COORDINATES: protein motif:HMM:NF021896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide isomerase DsbC N-terminal
                     domain-containing protein"
                     /protein_id="HYJ42009.1"
                     /translation="MTFKIAASLLVFCMGAGPAFAQTKAATPAAATTGPSAKADPRAA
                     IVKKIDGIKLEDVRMTPVSGVYEVTHGADISYLSGDGRYAILGDMIDLDSDSNLSETR
                     RRGIRARMIETVPENEM"
BASE COUNT         1708 a         3109 c         3039 g         1615 t
ORIGIN      
        1 cgatctgccc ttcgaaggcg gcgaccgacg aggtatggat gatgacgccg cgctcgccgt
       61 cagccgtggg cgtattcgcc tgcatgatgg cggcggccgc cttgtcgcac aggaatgtac
      121 cgatgaggtt gatgtgcacg acgcgcgtga agaaatcccc ggccatcggc ccgttcttgc
      181 cgagcacgcg gcccgcgccg atgacgccgg cgcaattcac cagcaggttg atgctgccca
      241 tggcggcgcg cgcggactcc attgcggtgt tgacctcggc ttccgaggtg acgtcgcact
      301 tggcaaagaa ggcgtttgcc ccaagctcgg ccgccgcagc ctggcccgga ccttcctgaa
      361 cgtctaacaa cgtgacgcgg ccaccggccg ccacgatgtg acgcgccacg gcattgccga
      421 gacccgaagc gccccccgtg accacggcgc gcgcgtctgc gtacttcatt ggaattcccc
      481 tttttgcgcg cacagcataa cgaaggcgcg cctcccgggc actccctcgc gcgtttcagg
      541 gcacctcgac cgcaatggcg gtggcttcgc cgccgccgat acataacgag gcaacgccgc
      601 gaccgccgcc gcgtgcacgc aaagcatgca ccagcgtggc aatgatgcgc gcgccggtcg
      661 ccccgatggg atgtccgagc gcgcacgcac cgccattgac gttggtgcgt gcgtggtcga
      721 agcccacgtc gcgcatggcg gccatggtga ccacagcgaa ggcttcgttc acctcgtaca
      781 agtggacatc gccggccttc cagccaagat gcgccagcag tttctggatt gcgccggcgg
      841 gtgccgtggt gaaccactcg ggttcctgcg cgaagctggt gtagccgtgg atgcgggcca
      901 gtggtttgat tccgcggcgc gcggcttcag cggccgacat cagcaccgcc gccgccgcgc
      961 catcggaaat cgacgacgaa gacgcggccg tgaccgtgcc atccttgcgg aacgcgggct
     1021 tgagcgttgc gatcttggcg aggtccacgg tgaatggcgt ctcgtccttg tcgaccaccg
     1081 tctcgccctt tcggcccttt acggtgacgg ggacgatttc gcccgcgaat gcgcccgagc
     1141 ccaccgcctt caatgcgcgc tgtgtcgatt cggtggcgaa cgcgtcctga tcggtgcggc
     1201 tgaacgagta tttctgcgcg gtggcttcgg caaacacgcc catcagctgt ccgtcgaacg
     1261 ggctctgcag gccgtcgtag aacatgtgat cgagcacttc accgtgcccc atgcgcatgc
     1321 cgccgcgtgc tttcggcagt agatagggcg cattggtcat ggactcgagg ccaccagcca
     1381 ccacgatgga cacgtcgccg gcgcgaatct ggtcggcggc catcatgatg gctttcattc
     1441 cggagccgca catcttgttg atggtggtgg tgggcacgcc cctgggcacc tccgcgccga
     1501 gtgcggcctg gcgcgcggga gcctgcccaa ggcccgcggg caacacgcag cccatgatga
     1561 cttcctggat gtcaccggct gaaactcccg cggccgcgag cgcacccctg atggcggccg
     1621 cgcccagttg tggcgccgtg actggcgaga acacgccctg gaatgcgccg atgggggtgc
     1681 gcgaggccgc ggcgatgact acctgtttgt tgtccatgaa aaactctccg tcagtcccgg
     1741 ccgcgaaggc ctctgttgaa aacatcgatg aagcgatcgg caatctcacc aggcttgagc
     1801 gtggcgtctt cgcgatacca gtgtcccatc cagttgagcg cgccggcgag cgcgaacgca
     1861 gtcattttcg tgtcgcaggc gcggatcgag ccgtcggcca cgccggcatc gatgagtgcg
     1921 cgcatgcgct tgtcgatgcc ggccttcagt agtttgatgc ggcggctcat cgccgcgccc
     1981 aggtgctggt cctcggcgcg cagcatgcac cacccgaaat ccccgacaat ggccgcggca
     2041 taaccgcgga tgaaggcgaa caggcgttca cgcgccgggc ccgcggcgct ctcgcactcc
     2101 ttgagcgtcg actggatctg cgccagacct gcccggaaac attcgaacag gatggcttcc
     2161 ttgctgggca catataaata gagtgtgggc ttggtgacct tgaggctggc ggcgagatcg
     2221 tccagcgagg tgttgtggta gccgcgctgg cgaaatgccc gcgctgcagc aaggatgacc
     2281 gcgtcgcgct tgagcgcatg gccgctgcca cgctggcggc gggcttccca cggcgaagcg
     2341 ggttcggatc gtttggcgcg ggcgggggcg ggcatgggcg ggtgtcaccg gcttcgtgtt
     2401 actcttgggt aacccatctt accagccagt aacatgaagc caccagagcc cggaaagccg
     2461 ctgcggttcg tcagcgccgc gtcgctgttc gacgggcacg acgccgccat caacatgatc
     2521 cgccgcctgc tgcaatccgg cggcgcggag gtggtgcacc tcggccacaa tcgcagcgtc
     2581 gccgacatcg tgggtgccgc cattgccgaa gacgccgatg ccatcgccgt cagcagctac
     2641 cagggcgggc acaacgaata tttccgctac atggtcgaca tgctgcgcga gcgcggcgcc
     2701 acgcatatcc gcgtggtcgt gggcggcggc ggcaccattt cgccggatga aatcgccgaa
     2761 ctcgaagcgt acggggtcgc caagatctac acgcccgagg acggacggag cctgggcctc
     2821 aatgggatga tcgccgatgt cttcgcgcgc gtcggccggc gcacgcatgc cgaccagatt
     2881 gcccgcatgc ccgcggtgca cgaccacggc gccatcgccc atgccatcac gctgctggaa
     2941 ggtggagccg gcgacgatgc ccgtggcgac actggcgcgg acgatttgcg gcggctgctg
     3001 agcgcgggca tcgccaatcc acagcgggcg ccggtcatcg gcctcaccgg cacgggcggc
     3061 gcgggcaaga gctcgctcac cgacgagtta ctgcagagat tcctgcgtca gttccccgat
     3121 cgcaacgtgg cggtggtcgc ggtagatccg acgcgccggc gctcgggcgg tgcgttgtta
     3181 ggtgaccgca tccgcatgaa ttcgctcgcg gcgtccaatg tgttcatgcg gtcgctggca
     3241 acccgccgcc agcatctcgc gaccagtgcc gtgctggcgg atgtcatcgc gctgtatcgc
     3301 gccgttggct tcgacctcgt cgtcgtggaa accgccggca tcggccagag cgacaccgag
     3361 atcgtcgacg tcgccgatct gtcgctgtac gtgatgacgg ccgaatacgg ggccgccagc
     3421 cagctcgaga agatcgacat gctcgacttc gccgacatga tcgtgctgaa caagttcgag
     3481 aaacgcggcg ccgaagacgc gctgcgcgac gtgcgcaagc aatggcgccg gaatcatccc
     3541 gatcgcatga agttgcccga cgaagacctg ccggtgtttc ccacgatcgc cagccgcttc
     3601 aatgatccgg gcgtaaaccg cctgttcctg gccctgtgca gcgcactcga cgaggagtgc
     3661 ggtgagacgg gccgctggac ctcggccgat ggggcgccgc tcgccgtcgc acctaccgag
     3721 ttcatctcgc gcgacccgct ggtgccgtcc gcgcggtccc gatacctcgc ggaaatcgcc
     3781 gcgcgtggcc gcgaggagca tggcaatgcc gtgcgccgtg cggatgcggc gcgacgtgca
     3841 cacggcctct atctatcgct gcaggcgctc aaggatccgg cgctcccgga accactggct
     3901 gccgcaaccg cgacgtccgg aaaagactcg gcactcgatg cgctgcgcac ggcatacaac
     3961 caggcgctcg acgacgttgg cgccgacgcg ctggcaagct tgcgcgcctg gccggcccgc
     4021 gagcatgcgg tgaccgcgcc cgagtattca tacacggtgc gcggccgtga ggtgcgcggc
     4081 gccaactaca cgacgtcgca gagccacacg ccgattccca agcttgcgcc gccgaaactg
     4141 tcgggctggg gcgagattct cgatttcctg aagaacgaga atcttcccgg cgcctggccg
     4201 tacaccggag gcgtgtatcc ctaccggcgc gaagaagaag acccgacgcg catgtttgcc
     4261 ggtgagggtg cgccggaacg caccaatcgc cgcttccact atctcgcggc cggtcacgag
     4321 gccacgcgcc tctccaccgc tttcgattcc actacgctct acggcgaaga tccggatatg
     4381 cggcccgaca tcttcgggcg caccggtaat tccggtgtat ccatcgctac gctcgatgac
     4441 atgaagaagc tctactcggg cttcgatctc tgcgccccgt cgacctcggt gtccatgacc
     4501 atcaatggcc cggcacccat gatcctcgcc atgttcatga acacggccat cgaccagcgc
     4561 gtggaacatt ttctgcggca gtcgaatcga tgggacgcgg tcgaggcggt gctgaagcgc
     4621 gccggcgcgg gaaccggcgc ctggccggct tatcgcggcg agctgccgcc ggaccacggc
     4681 ggtgaaggtt tgggattgtt aggtttcagt ggccgcaggc tggtggaact cgacttgctg
     4741 acctccgacg aatacacgcg cctgcgcgcc gaggcgctgt cgcgcgtgcg tggcacggtg
     4801 caggccgaca tcctcaagga ggaccaggcg cagaacacct gcattttctc aaccgagttt
     4861 gcgctgcgca tgatgggcga cgtgcagcag ttcttcagcg agaacgacgt acgaaatttc
     4921 tattcggttt ccatatccgg atatcacatc gccgaagccg gcgcgaaccc gatcacgcag
     4981 ctcgccttca cgctcgccaa cggtttcacc atcgtcgagt actacctggc gcgcggcatg
     5041 aagatcgacg acttcgcgcc gaacctgtcg ttcttcttct cgaatggcat ggatgcggaa
     5101 tacgcggtga tcggccgcgt ggcgcggcgc atctgggcgc gcgccatgcg cgacgtgtac
     5161 aaggcggggc cgcgcagcca gatgctgaag taccacattc agacatcggg tcgcagcctg
     5221 cacgcgcgtg aaatccagtt caacgacatc cgcaccacgc tgcaggccat gtatgcgctg
     5281 ttcgacaact gcaactcgct gcacaccaat gcctacgacg aggcgctgac tacgcccacc
     5341 gaggaaagcg tgcggcgcgc ggtcgccatc cagctcatca tcaatcgcga gttgggcctg
     5401 aatcgcatcc agaacccatg gtcgggctcg tatgcgatca cggcgctgac cgacctggtc
     5461 gaagaggccg tatacaaaga gttcgaatcg ctggccgaac gtggcggcgt actcggcgcc
     5521 atggaaacca tgtaccagcg cggcaagatc caggaagaga gcatgttgta cgagacgcgc
     5581 aaacacgacg gctctctgcc catcgtcggc gtgaacactt ttctgaatga tcggggcgcg
     5641 agcgaaaccg cggtagcgcc gctgttccgc gcctccgacg aagagcagcg cgcgcaggtc
     5701 gacgccgtgc atgccttcca ggcgcgccac gcgcaggtgg cgcccgaagc gttgcaaagg
     5761 ctgcaatcgg tcgcggcgag tggcggcaac atatttgccg agcttctgga taccgttcag
     5821 gtgtgctcgc tcggccagat cacgcgcgcg ctctacgaag tcggcggcca atatcgacgc
     5881 cacatgtagt gcggatcagc tgcgtgcgaa ctcgtcgatc tcggcaatcg ccgccttggc
     5941 gcgtgcgatg ctgtgactga ggagaaaggc gtccatccat gccgcgatcc tgccgtgctg
     6001 gcgacgggcc cagtaatgta gaaggggcgg tgcaagtccg cccgccaggc acaccaccaa
     6061 attggccagg aggaaaccgg acgcgcgatc ccagagatcc acacccgcga atcccatgac
     6121 gatggctacc aacgccgccg gcaccagtac ccagcaggca aaccacaccg cgtgaaacga
     6181 gcgcgcctcg aattcctgca gcgtcgccag cgagcgctgg atcttgagga ccggctgcgt
     6241 gtagtcgatc cgcagcacga ggaccaggcg caccacgccg atcacgatga ggccgatgct
     6301 cgccagatgt agcgcaatgc ccgcgaccgc cacggctgga gtggccagat gcgcgctcca
     6361 gaattttccc cacaccagcg cgaaccacgc gccgatgatc atgttcagta ccgcgccggc
     6421 cagcactggc gcaagtctcc atcgcacctt gcgcgagatc gtctcggtca atagccggcg
     6481 attgattgcg atgagatcct caacgcggcg atcgagactt tgccaggaag ccttcagttc
     6541 atcgagttcc atggtatttc tccgcgtagt tgcgcagcct gtctttcagg cggttgacct
     6601 tggtagttag attcgtggcg ctgaccccga gaatttccga catctcggag tatgggcgtt
     6661 cttcgaggta gagcagcaga acggctcggt cgagcgaatc ctgcgaggcg atgaagccac
     6721 gcagtatccg gcggccttcg tcgtcgtcgg ctgccccgtc ggtcgtgggc tcgtgcatgt
     6781 cggcgtcgta ggccacggag cgccggcgtc gcggcgcctc ggcgcgcagc caggagatcg
     6841 cgacgttgag cgcgatgcgg tacatccacg tggagaagag tcgcgcgcga tcgtaggccg
     6901 gaaacgcgcg ccagagttgt gccgcgattt cctgcgccag gtcttcgacg tccgcggtgt
     6961 cgcgcgcgta ggtgcgggcg accttgaaga tgaggcgccg gtggccatcg agcagcgccg
     7021 cgaactcgac tccaggttcg gggttcatgt acgtggactg ttgcatggtt tctgccgcca
     7081 tgattcgcgg caggaacccg gatgtcacag tcgggcttgc taggcggcca gatccggctc
     7141 gccgagctcc tgcaggatcg cctggcgcga ctgatcgcga agtctcaggg cggggtctgc
     7201 ttcgggccct gcgacagtcg gcgcgatggg cagaccgaag cgcacttcga gtgggcccgg
     7261 ccgtggcagt acgcgagtcg ccggcatgtt tcgccgcgtg ccgaggatca cggcgggaac
     7321 caccgggcaa gcggcgcgag ccgcgatcgc gaacgcgccg gtgtggaatt ttccgaggcc
     7381 gacctcgggg gtgaatgtgc cctcgggaaa gaacaccagc gactgcccgc tggccgccgt
     7441 ccgcaatacc cggcgcgcat cagtgccgcc cttgtggcga ttgaaacgat ccacgaactc
     7501 cgatccgatc cgccgcagca gcaggccggc gaccggcacc tcgttcatct cgcgcttgat
     7561 cacgaacgcg aatcgcggcg gcagggcggc agccatcacc acgccgtcga gatagctggc
     7621 gtggttggcg accacgacgc actggcccgc gggaaggttt tcagtgcccc gcaggcgaag
     7681 cggcatgccg gcgagaagaa agtaggttct tgctgcgatg cgggcggtgc cgcgtcgcaa
     7741 cttcaatgta ggcagaaaga ggacggcgag cagggcggtc agggctacaa gcaggaacaa
     7801 cagtgcggcg tagattccat aaatgaatcg caggggaacg gaaatggcgg ctggccaggg
     7861 tgcacgcacg atcggagaag tcgaagtcat tcgcgggcgt tcgccaaggg gggcctggga
     7921 ttcatgtgaa ctggttctct ttatgttaat ggttttgccg gggtgtgaag gtcatcacgc
     7981 ctaagcgctg gccgccgtcc ccatactgcc gtgacgcgtt aatccgcata cgcattaagg
     8041 caatcacgtc ttggtcaaac aagctacagc gcagaacgaa gcgcctgccg tcgacaccgc
     8101 cgacaccacc gtctcgaaat ttctcgacgc ggtctggatg gaacgtggac tgtctgcgaa
     8161 cacgctcgcc gcctatcgtg ccgacctgac cgcgctcgcg cgctggctgg ccgaacgggg
     8221 cacgcccatc atcaaaacga cgcgcgcgga cctgctcggt ttcatcgcat accgcgtcga
     8281 agccggtgcc cgccctcgct ccaccgcgcg ccagctttcg tctttccgtc gcttcttccg
     8341 ctacttcgtc cgcgaaggag tcatcaagga tgatccgact gctcagattg cgatgccgaa
     8401 gatcggccgc tcgctgccga agtcgctcac cgaagaagag gtcgaatcgc tgctgagcgc
     8461 gccggccatc gccgatccgc tgggccatcg cgatcgcacc atgctcgaag tgctgtacgc
     8521 cacgggcctg cgcgtttcgg agctcgtgaa cctcaagtac aaccagatca acaccaacca
     8581 gggtgtcatc cgcatcatgg gcaagggcaa tcgcgagcgt ttgattccgc tcggcgagga
     8641 agccgtgcgc tggctgacgg acttcgtgcg cgcgccgcgc agcgagatcc tgctcgaccg
     8701 caccaccgac tacctgttcc ccacgcgtcg cggtgatcgc atgacgcgcc aggccttctg
     8761 gcacatcatc aagcgctacg cccgcaaggc cgtcatcgac aaggaactgt cgccgcacac
     8821 gttgcgacac gcatttgcca cacacctgct caatcatggc gcggacctgc gcgtggtgca
     8881 gatgctgctc ggtcattcgg acctctcgac cacgcagatc tacacgcatg tggcccgtga
     8941 gcgcatgaaa gagatgcacg gtactcatca cccgcgaggg tgatgccggc gcggtgtccc
     9001 tgaggcgccg caaccccttg ttggagtaag attcgtcgcc tcgaccggcg cgcgaacttg
     9061 atcgtccggg ctcggtcgaa tctgcattac cgccgccaga gccacgagcg ccccatgacc
     9121 ttcaaaatcg ctgccagcct gcttgtcttc tgcatggggg ccgggccggc attcgcacag
     9181 acaaaggccg ccactccagc cgcggcaaca actggtccgt cggccaaggc cgatccgcgc
     9241 gcggccatcg tcaagaaaat cgacggcatc aaactcgaag acgtgcgcat gacgcccgtc
     9301 agcggcgtgt acgaagtcac gcacggcgcc gacatcagct acctctcggg cgatggccgc
     9361 tacgccattc tcggcgacat gatcgacctc gattccgact ccaacctttc cgagacccgg
     9421 cgccgcggca ttcgcgcgcg catgatcgaa accgttccgg aaaacgagat g
//
LOCUS       DASQJS010000367         7108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1800233, whole genome shotgun sequence.
ACCESSION   DASQJS010000367 DASQJS010000000
VERSION     DASQJS010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800233"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VEW08_14550"
     CDS             complement(<1..1168)
                     /locus_tag="VEW08_14550"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYJ42010.1"
                     /translation="MRFDAFELDEADARLKRDGKPVALPPKAFGVLCALARQPGVLVT
                     KNALLDTVWGHQHVSESVLKTTISQLRAALADDAASPRFIETASRLGYRFIGAKERVA
                     ALGAAPISEVEMEAAPFIGRRAPLARLQDSWSRAVGGQRQLVWVAGDAGVGKSTLLEN
                     FLRVSGAAAIAQGQCVEQYGAGEPYLPVLQALGDLCRAYPELAAIMRGVAPTWLLQLP
                     WLVNETERAALVRELVGVNQERMVREFHELIARFTEKQALLFVIEDLHWCDDATLRLM
                     DHFARQRGGARILWIGSFRLTQVIAESHPLQGLRQELRLHRLCDEIVLDPFSEAEVLE
                     YLRARMPMLEPPEAVVRRVHGHTDGLPLFVANVVDAVIGSEMDAGATSRQRMLAA"
     gene            1320..1706
                     /locus_tag="VEW08_14555"
     CDS             1320..1706
                     /locus_tag="VEW08_14555"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYJ42011.1"
                     /translation="MYSGINISQTDARPMYQQIMEQIRARIAAGDWPSGKELPSIRAL
                     AAALSVSVITIKRAYLDLENEGVIVTRHGKGSFVAEVNGLAGELQEEKLDEHLAAAAG
                     IGQQLGLSEEELATRLRRAIRTVDKK"
     gene            1703..2593
                     /locus_tag="VEW08_14560"
     CDS             1703..2593
                     /locus_tag="VEW08_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ42012.1"
                     /translation="MNPTKHIELRNVRKGFRFFTLEDLSLDLEPGQIMGFVGPNGAGK
                     STTIRLMTGLLAPDAGEIRVLGHAMPEAQALAKRDIGFVSDEMRLFGHATLGWHMNFV
                     SSIYPGWDAQYAKTLVRRFNLRPEQNVSGLSSGEHVKAVLLLALARRPRLLILDEPTS
                     GLDPVARHELLSEFMDVLRDEDRSILFSSHNTVDVERISDRITFIDRGRLIDSSDKED
                     FLERWRRIQLQLPAGATLPDLPHLVSRVDDGQFVTLTTNRYSEDLVAFLGNGVRQVQR
                     MSLEEIFVANVMHHRELRGQ"
     gene            2590..3258
                     /locus_tag="VEW08_14565"
     CDS             2590..3258
                     /locus_tag="VEW08_14565"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ42013.1"
                     /translation="MNMLTRKLIAKEFYLHRWLIIGSTVAGLVGLLIAAEGKVRFNIG
                     MLMWLTTIVAFGIVLAMFAIANERKERTLQFVLSLPLSHGDYVRIKVVGLLLCFLVPW
                     FILSAGAVTLIAVMPNLPDGLLPYAVLLCGFFLANYSVVLCSALHTASEGLMTLVIIT
                     TNMGITLFIFMVGALPSLNDHLFDPTPVWNSTFWTVLGAEFATLIVTLSLPYFVAARR
                     RDFI"
     gene            3271..5385
                     /locus_tag="VEW08_14570"
     CDS             3271..5385
                     /locus_tag="VEW08_14570"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ42014.1"
                     /translation="MNNPNEQRLHALDAVRGFALLLGVAFHAALSFMPGWPPGIWAMN
                     DNSPSQFLSDAAFVTHIFRMSLFFFIAGYFGRLLYQKLGASAFWANRGKRIAIPLVAG
                     WVVLYPLIGFIWVTGITKVFGGTMPPMPEMPKVPGAFPLTHLWFLYQLLLIYVAVIAA
                     RALIARFDRSERVRGLVDKIVAGSLRAPIAVFTLGLPVAAALMSLSFWFYWQGIPTPD
                     QSLIPQVPASVGFGTAFVFGWLVHRSRDALSLIASRWFVHLMLGVISTGWLLHTMHAS
                     PMAQPGLTKTLFAMIFGIAVWGWVLGLTGAALRFLSNFSATRRYIADASYWIYLAHLP
                     VVAAFQVWVGHWPLHWGVKYPFILIASFAVLFLSYHYMVRPTFIGQLLNGRKHPRKPL
                     PPGSTPSPASPPPAETVNGGPVAQLHGITKKFGAVTALNSIDIEVRRGELLAVLGPNG
                     AGKSTAISLWLGLIEPDAGVVTLLGGEPQDIARRRGLGVMMQDVEMPKELRVRELVDL
                     ASSYYSDPFEVDETLRRAGITQFADRPYGKLSGGQKRQAQFAVAICGRPKVLFLDEPT
                     VGLDIQARETMWRSIRALLADGCSIVLTTHYLEEAEALADRVAVVTKGRVIASGSVDD
                     MRSLVARRQISCESSLTADEVRGWPGVVDASRDSSKLQITATDAEGVVRRLLASDATL
                     TRLEVRQAGLNEAFNELTREAA"
     gene            5382..6176
                     /locus_tag="VEW08_14575"
     CDS             5382..6176
                     /locus_tag="VEW08_14575"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ42015.1"
                     /translation="MNALALKSIVVDSPVRGRAMGAYLTETRYELTRMIRNPGVAIPV
                     LVLPCALYALFALLIAGEAIDKDPSLGIFLFAAFSIMAVTMPALFGIGVTLALERDMG
                     LLRLKRAQPAPPAAWVVSKIVSGLILAVLAYAPILVLALATGKLSLETGQVAALSAAL
                     MVGTIPFCAMGLMIGALVSGTAAPAYANLIYLPGCYLSGMFFPLPKSMYWQAPIWPQF
                     HVEQFAMHLAGVTKYQFEPLQVAIATMLGYTVLFAGITIWRLKQKG"
     gene            6308..>7108
                     /locus_tag="VEW08_14580"
     CDS             6308..>7108
                     /locus_tag="VEW08_14580"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYJ42016.1"
                     /translation="MSGNDRREFLQKAGLAGVLSTIAPAAFAQSSGAFTVDPANTSQP
                     EIKPTHSISFAAIGLDHAHIFGMIAAIQRGGGTLVAFHATDPAQIADFNKKYPGVKLA
                     SEDEILNDKSIQLVAGAPIPDLRAPLGIRVMKAGKDYLADKPGITTLKQLADVRKAVK
                     ETGRRFAIMYSERLEVRSAVYAGELIKQGAIGRVVQTVNLAPHRVNAPSRPAWFFDKA
                     RYGGILCDIGSHQADQFLYYTNSTKAKVVAAQVGNVAYPQYPKFEDFGD"
BASE COUNT         1261 a         2367 c         2151 g         1329 t
ORIGIN      
        1 tcgctgcgag catgcgctga cgggatgtgg cgcctgcgtc catttcggac ccgatcaccg
       61 cgtcgaccac gttggcgacg aacaatggca gaccatcggt atgtccgtgc acgcgacgca
      121 cgacggcctc cggaggttca agcatcggca tgcgcgcgcg cagatactcg agcacttcgg
      181 cttcggaaaa cgggtcgagt acgatttcat cgcacaggcg atgcaaccga agctcctggc
      241 gcaaaccctg caacggatgg ctttcggcga tcacctgcgt aaggcggaaa ctgccgatcc
      301 acaggatgcg cgcgcctccg cgctggcgcg cgaagtgatc catgagccgc aacgtcgcgt
      361 cgtcacacca gtgcaggtcc tcgatgacga acagcagcgc ctgtttctcc gtgaaccgcg
      421 cgatcagctc gtgaaattcg cgcaccatgc gctcctggtt cacgccgacg agctcgcgca
      481 ccagcgccgc acgttccgtc tcattgacga gccacggcag ttgcagcagc caggtgggag
      541 ccacgccacg catgatcgcg gcaagctccg ggtaggcgcg gcacaggtcg cccagcgcct
      601 gcagcacggg tagatagggc tccccggcgc cgtattgctc gacgcattgc ccctgcgcaa
      661 tggcggcggc gccgctcacg cgcaggaagt tttcgagaag cgtgctcttg ccgacaccgg
      721 catcgcccgc tacccacacg agctggcgct ggccgccaac ggcacgggac caggagtcct
      781 gaagtcgcgc cagcggcgcg cgacgaccga tgaagggggc tgcctccatt tcgacttccg
      841 aaatgggggc tgcacccaga gccgcgactc gctccttcgc gccgatgaag cggtaaccca
      901 gccgggacgc cgtctcgatg aaccggggac tggccgcatc gtcggcgagc gccgcgcgca
      961 actggctgat ggtggtcttg agtaccgatt cgctgacgtg ctggtggccc cacaccgtgt
     1021 cgagcagcgc gttcttcgtc accagcactc ccggctggcg cgccagcgcg cacaacacgc
     1081 cgaaggcctt gggcggcagc gccaccggct tgccgtcgcg cttcaggcga gcgtccgcct
     1141 cgtccagctc gaacgcgtcg aatcgcaaac ggattgcact tgcctgcccc atcgaaacct
     1201 cgcgccaaat ttaacaggtg gatcgcgcgt cgatctaact acccggtgaa gtgcggaaaa
     1261 aatatttcgc gagggggctg gtattcgatt cggaactgag tatacactgt atacacagta
     1321 tgtacagcgg cataaacatc tcccagaccg acgcccgccc catgtatcag cagatcatgg
     1381 agcagatccg ggcccgcatc gccgccggcg actggccctc gggcaaggaa ttgccgtcaa
     1441 tccgggcact tgcagcggcc ctgagcgtca gcgtcatcac catcaaacgg gcctacctcg
     1501 acctcgaaaa cgaaggcgtc atcgtcacac gccatggaaa aggctcgttc gtggcggagg
     1561 tcaatggcct ggccggcgag ctgcaggaag aaaagctcga cgagcacctg gccgccgccg
     1621 ccggcatcgg ccagcagctc ggcctgtctg aagaagaact cgcgacccgc ctgcgtcgcg
     1681 ccatccgcac cgtggacaag aaatgaaccc gaccaaacac atcgaactgc gcaatgtccg
     1741 aaagggattc cgcttcttca cgctcgaaga cctgtcactg gatctcgagc cgggacaaat
     1801 catgggtttt gtcgggccga acggcgctgg caagagcacg acgattcggc tgatgaccgg
     1861 actgctcgcg cccgatgcgg gtgaaattcg cgtgctcggc cacgccatgc ccgaggccca
     1921 ggcgctggcc aagcgcgaca tcggattcgt atccgacgaa atgcggctgt tcggtcacgc
     1981 gacgctcggc tggcacatga atttcgtttc ctctatctac ccgggctggg acgcgcaata
     2041 cgcgaagacg ctggttcgcc gtttcaacct gcgtcccgag cagaacgtga gcggcctgtc
     2101 gagcggtgag cacgtcaagg cggtgttgct gctcgccctc gcccgccgcc cgcggctgct
     2161 gatcctcgat gagcccacct ctggcctcga cccggtggct cgccacgaat tgctcagcga
     2221 gttcatggac gtgctgcgcg acgaagatcg ctcgatcctg ttctcctcgc acaacaccgt
     2281 ggatgtggag cggatttcgg atcgcatcac gttcatcgat cgcggccgcc tgatcgacag
     2341 cagcgacaag gaagatttcc ttgaacgctg gcgccgcatc cagctgcagc ttcccgcggg
     2401 agcaacgctg cccgacttgc cgcacctggt cagccgtgtc gacgacggac agttcgtgac
     2461 gctcaccacc aatcgctact ccgaggatct ggtcgcgttc ctcggcaatg gtgtgcgcca
     2521 ggtgcagcgc atgagcctcg aggagatttt cgtcgcgaac gtcatgcacc accgggagct
     2581 gcgcggccaa tgaacatgct gacacgcaaa ctgatcgcga aggaattcta tctgcaccgc
     2641 tggctcatca tcggttccac ggtcgccggc ctcgtgggac tgctgattgc cgccgaaggc
     2701 aaagtccgtt tcaacatcgg aatgctcatg tggctcacca ccatcgttgc attcggaatc
     2761 gtgctggcca tgtttgcgat cgcgaacgaa cgcaaggaac gcacactgca gttcgtcctg
     2821 agcctgccgc tctcccacgg cgactatgtg cgtatcaagg tcgtcggcct gctgctttgc
     2881 tttctcgttc cctggttcat tttgagtgct ggtgcggtca cgctcatcgc ggtcatgccg
     2941 aatctgcccg acgggctgct gccatacgcc gtgctgttgt gcggattctt cctggcaaac
     3001 tactcggtgg tcctgtgcag cgcgctgcac acggcttcgg agggcttgat gaccctggtg
     3061 atcatcacca ccaacatggg catcacgctc ttcatcttca tggtcggtgc gcttccgtcg
     3121 ctcaacgacc acctgttcga cccgacgccg gtatggaact cgacgttctg gacggttctc
     3181 ggcgccgaat tcgcgaccct catcgtcacc ttgtcactgc cttacttcgt ggcggcgcgc
     3241 cgtcgcgatt tcatctgaag ggataaaaca atgaacaatc cgaatgaaca acgcctgcac
     3301 gcgctcgacg cggtgcgcgg cttcgccctc ttgttaggcg tcgcgtttca cgccgccctc
     3361 tcgttcatgc ccggttggcc gcccggcatc tgggccatga acgacaactc gccgagccag
     3421 ttcctgagcg acgcggcgtt cgtcacgcac atcttccgca tgtcgctgtt cttcttcatc
     3481 gcgggctact ttgggcgcct gctgtatcag aagctcggag ccagcgcttt ctgggcaaac
     3541 cgtggcaagc gcatcgcgat accgctcgtt gcgggctggg tcgtgttgta cccgctcatc
     3601 ggcttcatct gggtaaccgg catcaccaag gtctttggcg gcacgatgcc gcccatgccc
     3661 gagatgccga aagtgcccgg cgcctttccg ctcacgcatc tgtggttcct ctatcagctg
     3721 ctgctcatct acgtggcagt gatcgcggcg cgtgctctga tcgcgcgctt cgatcgcagt
     3781 gaacgcgtac gcgggctggt cgacaagatc gttgccggct cgctgcgcgc gcccatcgcc
     3841 gtgttcacgc tcggcctgcc ggtcgccgcc gcgctgatgt cgctgtcctt ctggttctac
     3901 tggcagggaa tcccgacccc cgatcaatcg ctcattccgc aggttcccgc atcggtgggc
     3961 ttcggcacgg cgttcgtatt cggctggctc gtgcatcgct cgcgcgatgc cctgtcgttg
     4021 atcgcaagcc gctggttcgt acacctgatg ctgggcgtga tctcgaccgg ctggttgctg
     4081 cacaccatgc atgcctcgcc catggcacag cccggcctca ccaagacctt gttcgcgatg
     4141 atcttcggca tcgcggtctg gggctgggta ctcggcctca ccggcgccgc gctgcgattt
     4201 ctctctaact tcagcgccac tcgccgctac atcgccgacg cctcgtactg gatctacctg
     4261 gcgcacctgc cggtggttgc ggccttccag gtctgggtgg gccattggcc gctgcactgg
     4321 ggcgtcaagt atccgttcat cctgatcgcg agtttcgcgg tgctgttcct gagctatcac
     4381 tacatggtgc ggccgacctt catcggtcag ttgctcaacg gccgcaagca tcctcgcaag
     4441 ccgctgccgc ccggctccac gccctcgccg gcctcaccgc cgcccgcgga aaccgtcaac
     4501 ggcggccccg tcgcgcaatt gcacgggatc accaagaaat tcggcgccgt cactgctctc
     4561 aactcgatcg atattgaagt gcgccgcgga gaattgttgg cggtgctcgg ccccaacggc
     4621 gccggcaagt ccacggcgat ctcgctgtgg ctcgggctca tagagccgga tgcgggcgtc
     4681 gtgacactgc tcggcggcga accgcaggac atcgcgcggc gccgtggact cggtgtgatg
     4741 atgcaggacg tcgagatgcc caaggaactg cgagtacgtg aactcgtcga tctcgcgtcg
     4801 agttattact cggacccatt cgaggtcgat gaaactctgc ggcgcgccgg catcacgcag
     4861 ttcgccgatc gcccctacgg caaactgtcc ggcggccaga agcgccaggc gcagttcgcg
     4921 gtggcgatct gcggccggcc gaaggtgctg ttcctcgacg agccgaccgt ggggctcgac
     4981 atccaggcgc gcgagaccat gtggaggagt attcgtgcgc tgctcgccga tggttgttcc
     5041 atcgtgctca cgacgcacta tctggaagaa gccgaagcgc ttgcggaccg cgtcgcagtc
     5101 gtcaccaagg gccgagtaat tgcctccggc agcgtggatg acatgcgctc cctggtcgcg
     5161 cgacggcaga tcagctgcga gtcgtcattg accgcggacg aagtgcgcgg ctggccaggc
     5221 gtcgtggacg ccagccgtga ctcatcgaaa ctgcagatca ccgccaccga cgccgaaggc
     5281 gtggtgcgcc gcctgctggc aagcgatgca acgctgacgc gcctcgaagt gcgccaggcc
     5341 ggactcaacg aagcattcaa cgaactcaca agggaggctg catgaacgcg ctggctctga
     5401 aatcaatcgt cgtcgattcc cccgttcgcg gccgcgccat gggcgcgtac ctcacggaaa
     5461 cccggtacga gctcacgcgc atgattcgca atccaggcgt tgcgatccct gtgctggtac
     5521 tgccttgtgc cttgtacgcg ttgtttgcgc tgctgatcgc tggcgaagcg atcgacaagg
     5581 atccgagcct cggcatcttc ctgttcgccg ccttttcgat catggcggtg accatgccag
     5641 ccctgtttgg tatcggcgtc acgctcgcgc tggaacgcga catgggcctg ttgcgcctca
     5701 agcgtgcgca gccggcgccg cccgcggcat gggtggtctc gaaaatcgtc agcggcctga
     5761 tactcgcggt gctcgcgtac gcgcccatcc tcgtcctggc actcgccacc ggcaaactgt
     5821 cgctcgagac cggccaggtg gccgcgttga gcgccgcgct gatggtgggc acgattccgt
     5881 tctgtgccat ggggctcatg atcggtgcgc tggtgagtgg caccgctgcg cccgcgtacg
     5941 ccaacctcat ctatctaccg gggtgttacc tttcgggcat gtttttcccg ttgccgaagt
     6001 ccatgtactg gcaggcgccg atctggccgc agttccacgt cgagcagttc gcgatgcacc
     6061 tcgccggcgt caccaagtac cagttcgaac cgctgcaagt ggcgatcgcc accatgcttg
     6121 gttacaccgt gctgttcgcg ggcatcacca tctggcgcct gaagcagaag ggatgaaatt
     6181 aacggggtca ggcaccgttt cctggaaaac ggtgcctgac accctttaat acttacggcg
     6241 taggagccgt gccatcttcg ggacgacgcg gtatctttgc cgccgcacaa ccgaaggggc
     6301 gcgagacatg agcggcaacg atcgacgaga gtttctgcag aaggccggac tggccggcgt
     6361 cctctcaacc atcgccccgg ccgccttcgc gcagagctcc ggcgccttca cggtggaccc
     6421 ggcgaacacc tcgcagcccg agatcaagcc gacgcactcc atcagctttg cggccatcgg
     6481 gctcgaccac gcgcacatct ttggaatgat cgcggcgatc cagcgcggcg gcggcacgct
     6541 cgtggcattc cacgccaccg atccggcgca gatcgcggat ttcaacaaga aataccccgg
     6601 cgtgaagcta gcgagcgaag acgaaatact caacgacaag tccatccagc tggtcgccgg
     6661 cgcaccgatc ccggacctgc gcgcgccgct cggcatccgc gtgatgaaag ccggcaagga
     6721 ctacctggcg gacaagccag gcatcaccac gctcaagcag ctcgcggacg tccgcaaggc
     6781 cgtgaaggaa accggccgca ggttcgccat catgtattcc gagcgcctcg aagtgcgctc
     6841 cgccgtgtat gccggcgagc tcatcaagca aggcgccatc ggccgcgtgg tgcaaaccgt
     6901 gaacctcgcg ccgcatcgcg tgaacgcgcc ctcccgcccg gcctggttct tcgacaaggc
     6961 gcgctacggc ggcatactct gcgacatcgg gtcgcaccag gccgatcaat tcctctatta
     7021 caccaacagc acgaaggcca aggtggtcgc ggcacaggtg ggaaatgtgg cctacccgca
     7081 gtacccgaag ttcgaagact tcggcgac
//
LOCUS       DASQJS010000368         4451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4626247, whole genome shotgun sequence.
ACCESSION   DASQJS010000368 DASQJS010000000
VERSION     DASQJS010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4626247"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1440
                     /gene="hflX"
                     /locus_tag="VEW08_14585"
     CDS             97..1440
                     /gene="hflX"
                     /locus_tag="VEW08_14585"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF008280.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HYJ42017.1"
                     /translation="MFERPRAGERAVLVRLGIGAPADPEDILEFEALTRSAGATPVVS
                     IGGRRDRPDPRFFVGSGKADEIRAAAAEHNADVVLFDHPLSPSQERNLEKLIERRVLD
                     RSGLILDIFAQRARSHEGKLEVELAQLKHIASRLVRGWTHLERQRGGGIGNRGPGETQ
                     LETDRRLLAKRVKTLDARLEKIKQQRETGRQQRAEIPVPSLALVGYTNAGKSTLFRAL
                     TGADAYIADQLFATLDPTVRSIVLPGNTKVVVADTVGFIRELPHELVAAFQSTLTEAR
                     EATLLLHVVDVSDPRRDEHIDEVNRVLAEIGAADLPQILVFNKIDRIETPARAERDES
                     GKVSHVWLSAAKADGLDLLRESVAERLARVARRATLRLPVSAGELRARLYAANLVRAE
                     RIADDGAFEIDTELSEVELLKWAREPGVEVLRLADDSTCTVAGAYLQSSPALQSS"
     gene            1442..2539
                     /gene="hflK"
                     /locus_tag="VEW08_14590"
     CDS             1442..2539
                     /gene="hflK"
                     /locus_tag="VEW08_14590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HYJ42018.1"
                     /translation="MAWNQPGNSNNNPWGKKPAPGGGDFDQAFKDWQKRIETLFGGGG
                     GRSSTPLLFIIIIGALIWMLTGFYQVQPGNQGVVQRFGRKVEIVGDGWGWRLPWPIET
                     VTKVDVAGVKSIAYKSRVLTAEPNMVELQVAVQYRITRAEDYLFRVREPIATLGEVSE
                     SAIREVVGRNDQQAILEAGRIKIAEDTRVIMQRTLDQYGAGIEVRSVNITDVQVPEAV
                     QQAQRDSVKAKADRERMIKEAQAYANGIIPVAQGNAARAVQEAEAYKSQVVSMATGDA
                     SRFSQLAVAYEKAPNVTRERLYIETVEAVLKASRKVVIDSKSGNGNMLYLPLDKLMDR
                     RDGDGTVTVRPPVAVEPDPSLAPDPRNRVER"
     gene            2539..3414
                     /gene="hflC"
                     /locus_tag="VEW08_14595"
     CDS             2539..3414
                     /gene="hflC"
                     /locus_tag="VEW08_14595"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HYJ42019.1"
                     /translation="MGSRILGILVVVFAAVVLISMSTFVVGEQEMAVRVRLSQIKGAD
                     FKPGLQFKIPFMDRVVKFDRRVLTRKFDGEQFLTNESQGLTIDYYIKWRILDAEQFYQ
                     ATSGGDEGRAETLVGETIQDGIKNAVAGRTLAEIVISDRQELTGEFMKNATERLLKLG
                     IQLVDVRVQRIDLQEEVATSVYESMKRTFEGIARTRRGEGDRESQIVKAEAERKKTEL
                     IARALADAQRIRGEGDAAAAAIYATAYNRNPEFYSFYRSLQAYKNSLGKEGDVMVVSP
                     DSEFFRYFKNPAPTR"
     gene            3467..>4451
                     /locus_tag="VEW08_14600"
     CDS             3467..>4451
                     /locus_tag="VEW08_14600"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HYJ42020.1"
                     /translation="MGKFVIIIGTQWGDEGKGKVVDLLTERAAAVARFQGGHNAGHTL
                     VIGGKKTVLSLIPSGILHKNVLCLIGNGVVLSLEALFREAQTLIDTGVDVFARLRVSP
                     NCPLILPSHVALDKAREAARGANAIGTTGRGIGPAYEDKVARRAVRVQDLFQREKFAA
                     KLGEILDFHNFVLQHYFKQPAVDFQKTLDEQLSYADRVRPLVNDVTQTLAALREQRAD
                     VMFEGAQGAMLDVDHGTYPFVTSSNTTGGFAGTGTGIGPRSFDYVLGIVKAYTTRVGA
                     GPFPTELFDEYGEHLSRVGHEFGAVTGRKRRCGWFDAVALRRSIIHSSVSGL"
BASE COUNT          851 a         1449 c         1429 g          722 t
ORIGIN      
        1 gtgcgcatca ccaacgaaga cggaacgacg atcgaggcgg tcgccggcga gtgatttacg
       61 cggtcgcacg cgtttccgtt ttctcgagaa tccagattgt tcgagcgacc acgcgcaggt
      121 gagcgggccg tgctcgtgcg ccttggcatc ggcgcacccg cggaccccga agatatcctg
      181 gagttcgaag cgctcacgcg ctccgccggt gccactcccg tggtcagcat cgggggccgc
      241 cgtgatcgtc ccgatccgcg tttcttcgtg ggcagcggca aggccgacga aatccgcgcg
      301 gcagcggccg aacacaatgc cgacgtcgtg ctcttcgatc acccgctttc gccctcgcag
      361 gaacgcaatc tcgaaaagct catcgagcgc cgcgtgctcg atcgctcggg gctgatcctc
      421 gacatcttcg cgcagcgtgc acgcagccac gaaggcaagc tcgaagtgga gctcgcgcag
      481 ctcaagcaca ttgcctcgcg cctggtgcgc ggctggaccc acctcgagcg ccagcgcggt
      541 ggcggcatcg gcaaccgtgg acccggtgaa acccagctcg aaaccgaccg ccgcctgctt
      601 gccaagcgag taaagacgct cgacgcgcgg ctcgagaaga tcaagcaaca gcgcgagacg
      661 ggccggcagc aacgcgccga gatcccggtt ccgtcgctcg ccctggtcgg gtacaccaac
      721 gccggcaagt ccacgctgtt ccgcgcgctc accggcgccg acgcctacat cgcggaccag
      781 ctgttcgcga cgctcgatcc gacggtgcgc agcatcgtgc tgccgggcaa caccaaggtg
      841 gtggtggccg acaccgtcgg tttcatccgc gagctgccgc atgagctggt ggccgctttc
      901 cagtcgacgc tcaccgaagc ccgcgaagca acgctgctgc tgcacgtggt cgacgtcagc
      961 gacccgcggc gcgatgaaca catcgacgag gtgaaccggg tgctggccga gatcggcgcg
     1021 gccgacttgc cgcaaatcct ggttttcaac aagatcgatc gcatcgagac gccggcgcgc
     1081 gccgaacgcg acgaatcggg caaggtgagc cacgtctggc tgtctgccgc gaaggccgat
     1141 gggctcgacc tgctgcggga atcggtcgcc gagcgcctgg cccgcgtggc gcgccgtgcg
     1201 acactgcgcc tgccggtgtc ggcgggtgaa ctgcgggccc ggctgtatgc cgcaaacctg
     1261 gtccgggccg aacggatcgc cgacgacggg gccttcgaaa tcgacactga actatccgaa
     1321 gtcgagctgc tcaaatgggc gcgagagccc ggcgtcgaag ttctaaggct tgccgacgac
     1381 tcaacttgta cggtcgcagg tgcctaccta caatcgtcgc ccgcattgca aagcagctga
     1441 catggcctgg aatcaacccg gtaattcgaa taacaaccct tggggcaaga aaccggcccc
     1501 cggtggtggc gatttcgatc aggccttcaa ggactggcag aagcgcatcg aaacgctttt
     1561 cggaggtggg ggcgggcgat cgtccacgcc gctgctgttc atcatcatca ttggcgccct
     1621 gatctggatg ctgaccggct tctaccaggt gcagccgggt aaccagggtg tcgtgcagcg
     1681 tttcggcaga aaagtcgaaa tcgtgggtga cggctggggc tggcgcctgc cgtggcccat
     1741 cgaaacggtc accaaggtgg acgtcgcggg cgtcaaatcc atcgcctaca agtcgcgcgt
     1801 gctcaccgcc gaaccgaaca tggtcgagct gcaggtcgcc gtgcagtacc gcatcacccg
     1861 cgccgaggac tacctgttca gggtgcgaga gcccattgcc acgctcggcg aagtcagcga
     1921 gagcgccatt cgtgaagtgg tgggccgcaa cgaccagcag gccatcctcg aagccggccg
     1981 catcaagatc gccgaagaca cgcgcgtcat catgcagcgc acgctcgatc agtacggcgc
     2041 cggcatcgaa gtgcgcagcg tgaacatcac cgacgtgcag gtgccggaag cggtgcagca
     2101 ggcgcagcgg gattcggtca aggccaaggc cgaccgcgaa cgcatgatca aggaggccca
     2161 ggcatacgcg aacggcatca tcccggtcgc ccagggcaac gccgctcgcg cggtccagga
     2221 agcggaagcg tacaagtcac aggtggtttc gatggcgacc ggcgatgcct cgcggttctc
     2281 gcagctggca gttgcctatg aaaaggcgcc gaacgtgacc cgcgagcgcc tctacatcga
     2341 aactgtcgaa gccgtactca aggcatcgcg caaggtcgtc atcgattcga agagcggcaa
     2401 cggcaacatg ctctatctac cgctcgacaa gctcatggat cgccgcgatg gcgacggcac
     2461 ggtcacggtg cgcccgccgg tggccgtaga gccggatccg agcctcgctc ccgacccgcg
     2521 caacagggtg gaacgctgat gggctcgcgc attctcggaa tcctcgtagt ggtgttcgcc
     2581 gcggtcgtgc tcatctcgat gtcgacattc gtggtcggcg agcaggagat ggcggtgcgt
     2641 gtgcgcctgt cacagatcaa gggggcggat ttcaagcccg gcctgcagtt caagatcccg
     2701 ttcatggacc gtgtcgtcaa gtttgaccgc cgcgtattga cccgaaaatt cgacggcgaa
     2761 cagttcctga ccaatgagag ccagggactc accatcgact actacatcaa gtggcggatc
     2821 ctcgatgccg agcagttcta ccaggcgacc agcggcggcg acgaaggccg cgccgagacg
     2881 ctggtgggcg aaaccatcca ggacggcatc aagaacgcgg tggcgggccg cacgctcgcg
     2941 gaaatcgtga tctcggatcg ccaggaactg accggcgagt tcatgaagaa cgccaccgag
     3001 cgcctgctga agctcggcat ccagctcgtg gacgtgcgcg tacagcgcat cgacctgcag
     3061 gaagaggtcg ccaccagcgt gtacgagagc atgaagcgca ctttcgaggg catcgcgcgt
     3121 acacgccgcg gcgaaggcga tcgcgaatca cagatcgtca aggccgaggc cgagcgcaag
     3181 aaaaccgagc tcatcgcccg ggcgttggcg gacgcgcagc gaattcgtgg tgaaggtgat
     3241 gccgccgccg cggccatcta cgccaccgcc tacaatcgca atcccgagtt ctattcgttc
     3301 tatcgcagcc tgcaggcgta caagaactcg ctgggcaagg aaggcgatgt gatggtggtt
     3361 tcacccgaca gcgaattctt ccgctacttc aagaatccgg cgccaacgcg ctgacgccat
     3421 ccaccttcgc acctgatttc gtcgtgaaac gcggcacaca ttactaatgg gcaaattcgt
     3481 catcatcatc ggcacccagt ggggtgacga aggcaagggc aaggtcgtcg acctgctcac
     3541 cgaacgcgcc gccgcggtgg cgcgcttcca gggcggacac aacgccggcc atacgctggt
     3601 catcggcggc aagaagaccg tcctgtcgtt gatcccctcg ggaatcctcc acaagaacgt
     3661 gttgtgcctg atcggcaacg gcgtggtgtt gtcgctggaa gcgctgttcc gcgaagccca
     3721 gacactcatc gacaccggcg tggacgtgtt cgcccgcttg cgagtctcgc ccaattgccc
     3781 gctgatcctc ccctcgcatg tcgccctgga caaggcgcgc gaagcggcgc gcggcgccaa
     3841 cgccatcggc accaccggtc gcggcatcgg gcccgcctac gaggacaagg tcgcgcggcg
     3901 cgcggtgcgc gtgcaggacc tgttccagcg cgagaagttt gccgccaagc ttggcgagat
     3961 cctcgatttc cacaatttcg tgctgcagca ctacttcaag cagccggcgg tcgatttcca
     4021 gaagacgctg gacgagcagc tgtcgtacgc ggaccgcgtg cggccgctgg tcaacgacgt
     4081 cacccagacg ctggcggcac tgcgtgagca gcgcgccgac gtgatgttcg aaggcgcgca
     4141 gggcgcgatg ctcgacgtcg accacggcac ctatccgttc gtcacttctt cgaacaccac
     4201 gggcggtttc gccggcaccg ggaccggcat cggtccgcgc tcgttcgatt acgtgcttgg
     4261 catcgtgaag gcctacacca cgcgtgtcgg cgcgggcccg ttccccaccg agctgttcga
     4321 cgaatacggc gagcacctct cgcgagtcgg gcacgaattc ggcgcggtca ccgggcgcaa
     4381 gcgccgctgt ggctggttcg atgccgtggc gttgcgccgc tccatcatcc attcgagcgt
     4441 gtcaggcctg t
//
LOCUS       DASQJS010000369         4842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_859465, whole genome shotgun sequence.
ACCESSION   DASQJS010000369 DASQJS010000000
VERSION     DASQJS010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859465"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="VEW08_14605"
     CDS             <1..1527
                     /locus_tag="VEW08_14605"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYJ42021.1"
                     /translation="MSSRRPPDSTLPRRQFLGAAAAATAFTLVPRHVLGGPRRIAASD
                     RVNIAGVGVGGMGRANMQALSGMNLVAMCDVDWSYVDTRYADIPKQIEAANKRLTEAT
                     DAKQKDQLKTQIANWQQLQEKWPKTRRYTDYREMFEKQKNIDAVVIATPDHTHAHIAL
                     AAMDLGKHVYVQKPLAWSVEECRRLAAKATATKLQTQMGNQGHSSDDARLVNEYIQSG
                     AIGTITEVHVWTNRPLAYWPQGIPRPAPLTANPKDLPWNMNGVMTRAAGSFGSYPPPD
                     KLAWDLFLGPSRYIEYHPIYHPFNWRGWTDWGVGAIGDMGAHLIDSSFWSLDLDYPTS
                     IETTSTPYNHDTYPMASTTYYEFPAKGARPAVKMTWYDGGLTPTKPVEMGDEELNKGG
                     GVLYIGTRGKLLHDTYGFNPRLLPKSLHESVGKPKETYARITTSHEMNWIDAIRGTQK
                     TTSPFEYSAKLTEIMLLGVAALNAGRKIDYDGAAMRVTNVPDSDALLKRKYRAGWELS
                     "
     gene            1702..2535
                     /locus_tag="VEW08_14610"
     CDS             1702..2535
                     /locus_tag="VEW08_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42022.1"
                     /translation="MKWIALVATSAISLASLAAAAAPAKSPTIEKSPDLLHAPQLNKS
                     AVRAAAPLLLAPGVTAAAAPTVEDVGDADSFGRNVTYLGLAQTLAVTVLDDCSASDPT
                     VERCIVANPPPAPTSFNEDGLATINLPAKATKSLICFALTPLLQVQWENNLATPAQAR
                     FSGTALISIDNEVLDDPALIDPGTGLPFGGVLALGLSTFTDIHTLQPGETDFKSMQMS
                     RSCIAGIVSKRVLVDGYGLTETQATQFFKKPMTITFGARGTFALSSFASYFYGMRLYG
                     D"
     gene            2661..2861
                     /locus_tag="VEW08_14615"
     CDS             2661..2861
                     /locus_tag="VEW08_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42023.1"
                     /translation="MRLKRLLIGISTILLVATGTISCVTQPFVRPAALERHVRVLAVT
                     LHPRSVDTIAAVRALHDVAMEF"
     gene            2867..3772
                     /locus_tag="VEW08_14620"
     CDS             2867..3772
                     /locus_tag="VEW08_14620"
                     /inference="COORDINATES: protein motif:HMM:NF019610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin"
                     /protein_id="HYJ42024.1"
                     /translation="MNPERRSLLQLLACAPLASCSGSGPAPQTPSGSDAISKIKPLFK
                     PKRPPVPGDWLETHKEPGQTYQQFRAITPTPAASRYSTLRIVPIGTLSDGQQEVVNVV
                     IDFLQPFFGLELAISPAVPIDAIPEDSTRRQSLDGPPQLLTTYLLNSVLLPLRQPADA
                     AVLAITAADLWPGPGWNFVFGQASLKERVGVWSMARNGDADGRADMRKLCALRTTMTA
                     THETGHMFGIRHCIAYECGMNGSNHSEERDRQPLEFCPECQPKLWWTCALDPLERSRA
                     LAAVARRHGFPKLAGAFDRQSQALA"
     gene            3851..4354
                     /locus_tag="VEW08_14625"
     CDS             3851..4354
                     /locus_tag="VEW08_14625"
                     /inference="COORDINATES: protein motif:HMM:NF025912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ42025.1"
                     /translation="MNPPDLQQIRAAVAQDPRNAELRYLLGAELAQQKEYEAAVVEMS
                     TALQLDPLLHFARFQLGLLYLTMAQPNQSLEIWAPLESLGETAALKFFKRGLEALIRD
                     DFDTCVGMLRHGIELNTQNAPLNYDMTQVIDRVDEALVPAQSITVASMSLDAGFSRYG
                     NPGKTRH"
     gene            complement(4390..>4842)
                     /locus_tag="VEW08_14630"
     CDS             complement(4390..>4842)
                     /locus_tag="VEW08_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42026.1"
                     /translation="QYTFIAVVDGEVGGAAVDFQDRAAVAKSLSVEVVDAAGKARKVV
                     PLEPAPEDVQPLLKTLVPAMGAAMGNLGRNLHFFVFQDQVENARLFSPFETGAVVVKL
                     SGRTSRQDISLSIDRPIDSLLVPRLCANGKPAHVSWNFCPWDGKKLPQ"
BASE COUNT          929 a         1628 c         1431 g          854 t
ORIGIN      
        1 atgagctcgc gcagaccccc ggattcaacc cttcctcgac ggcaatttct cggcgccgcc
       61 gccgcggcca cggcatttac ccttgttcca cgccacgtgc tgggcgggcc ccgccgcatc
      121 gcagccagcg atcgggtcaa catcgccggc gtcggcgtcg gtggcatggg ccgcgccaac
      181 atgcaggcgc tgtcgggcat gaatctcgtc gccatgtgcg acgtggactg gtcatacgtc
      241 gacacgcgtt atgccgacat tccgaagcaa atcgaggcgg cgaacaaacg actgaccgag
      301 gccaccgatg cgaagcagaa agaccagcta aagacgcaga tcgcgaactg gcagcagttg
      361 caggagaagt ggccgaaaac caggcgctac accgactacc gcgagatgtt cgaaaagcag
      421 aagaacatcg acgcggtggt cattgccact ccggaccaca cgcacgcgca cattgcgctc
      481 gcggccatgg atctcggcaa acatgtgtac gtgcagaagc cgctcgcatg gtccgtcgag
      541 gaatgccgcc ggctcgccgc gaaggccact gccaccaaac tacagacgca gatgggcaac
      601 caggggcatt cctccgacga tgcgcgtctg gtcaacgaat acatccagtc cggcgccatc
      661 ggcaccatca ctgaagtgca cgtgtggacc aatcggccgc tggcgtattg gccgcagggc
      721 attccgcgcc ctgcgccgtt gaccgcgaac cccaaagacc ttccgtggaa catgaacggc
      781 gtgatgacgc gcgcggccgg gtctttcggc tcgtacccgc caccggacaa actcgcctgg
      841 gacctgttcc tgggcccgtc gcgctacatc gaatatcacc ctatctacca cccgttcaac
      901 tggcgtggct ggaccgactg gggcgtgggt gccatcggcg acatgggcgc gcatctcatc
      961 gattcttctt tctggtcgct cgatctcgac tatccgactt ccatcgagac cacttcgacg
     1021 ccttacaacc acgatacgta tccaatggcg agcaccacct attacgagtt cccggcgaaa
     1081 ggcgcccggc ccgccgtgaa gatgacctgg tacgacggcg gcctcacgcc gacgaagccc
     1141 gtcgaaatgg gcgacgagga actcaacaag ggcggcggtg tcctgtacat cggcaccagg
     1201 ggcaagctgc tgcatgacac ctatggattc aatccgcgcc tgctgccgaa gtcgctgcac
     1261 gagagtgtgg gcaagccgaa ggagacctac gcgcgcatca ccaccagcca cgagatgaac
     1321 tggatcgacg cgatccgcgg cacgcagaag accacgagcc cgttcgaata ctcggccaag
     1381 ctcaccgaga tcatgttgtt aggcgtggcc gccctcaatg ccggcaggaa aatcgactac
     1441 gacggcgccg ccatgcgggt gaccaacgtg ccggactcgg atgcgttgct caaacgcaag
     1501 taccgtgctg gctgggaatt gtcgtagcgc gtgaaaattt acgcgggcaa ctcctaggcg
     1561 gagtgttccg cgcgcacctt tcgccggaaa taagtctcaa ctttcacact cacgcgcgcc
     1621 ggtgatccgc cggcgcgtgt tcctccccgt aagttccggc tgcgacggcg ttcgctgccg
     1681 ggatgttcag gaggaacacg aatgaaatgg attgcactgg ttgccacgtc ggcaatctct
     1741 ctcgcgtcgc ttgcggcagc tgctgcgccc gccaaatcgc ccaccatcga aaaatcgccc
     1801 gacctgctgc acgcgccgca gctcaataaa tccgccgttc gtgccgctgc accgctgctt
     1861 ctcgccccag gcgtgacggc cgcggccgcg cctaccgtcg aggatgtagg cgacgcggac
     1921 tcgttcggcc gtaacgtgac ctacctgggg ctcgcccaaa ccctggcggt gactgtgctc
     1981 gacgactgct ccgccagcga tcccacggtc gagcgctgca tcgtcgcgaa tcccccgccc
     2041 gcacccacgt cgttcaacga agacggcctc gccaccatca acctgcccgc caaggcaacg
     2101 aaatcgctga tctgctttgc gctcacgccg ctactccagg tgcagtggga gaacaatctc
     2161 gccacgccag cgcaggcgcg tttctccggc accgcactca taagcatcga caacgaagtg
     2221 ctcgacgacc cggcgctcat cgatccgggt accggcctgc ccttcggcgg cgtgcttgcg
     2281 ctgggcttga gcacctttac cgacatccac acgctgcagc ccggtgagac cgacttcaag
     2341 tccatgcaga tgtcgcgctc gtgcatcgcc ggcatcgtga gcaagcgggt attggtcgac
     2401 ggctatggcc tgacagagac acaggcgacg cagttcttca agaagccgat gaccatcacc
     2461 ttcggcgcgc gcggcacgtt cgcgctgtct tcgttcgcta gctacttcta cggcatgcgc
     2521 ctgtacggcg actgaccgac aacgagattg gcgccggacc tcttcccgag acttgcaacc
     2581 tggcgccatg ggcttcgcgt gcctcaaccc cgcgcgaggc cctttttttg tccgattcaa
     2641 gctagagtgc gcctcacaac atgcgcctca agcgtctact catcggaatc tcaacgatcc
     2701 tgctggtggc gaccgggacc atttcctgcg ttacccaacc gttcgtccgt cccgccgctc
     2761 tcgagcgcca cgtgcgcgtg ctggctgtca ccctgcaccc gcgcagcgtc gacaccatcg
     2821 ccgccgtgcg cgccttgcac gacgtggcga tggagttctg aacagtatga atcccgagcg
     2881 acgctcattg ctgcaactgt tggcatgcgc accactggcc tcgtgctccg gttcgggccc
     2941 ggcgccacag acgccttccg gctcagatgc catttcgaag atcaaaccac tgttcaaacc
     3001 caagcggccg cctgtgccgg gcgactggct ggagacgcac aaggagccgg ggcagaccta
     3061 ccagcagttc cgcgccatca cacccactcc cgcggcgagt cggtattcaa cgctgcgtat
     3121 cgttcccatc gggaccttga gcgacggcca gcaagaggtc gtgaacgtgg tcatcgactt
     3181 cctgcagccc tttttcggac tggagctcgc cattagcccg gcggtgccga tcgacgcaat
     3241 ccccgaagat tcaacgcgac gccaaagcct ggatggaccg ccgcagttgc tgaccaccta
     3301 tctactgaac tcggtactgc tgcccctgcg ccagccggcc gacgccgccg tgctggcaat
     3361 cacggccgcc gacctctggc ctggtcccgg atggaatttc gtattcggac aggcctcgct
     3421 caaggaacgt gtcggggtct ggtcgatggc gcgcaatggc gatgccgatg gccgcgcgga
     3481 catgcgcaag ctgtgcgccc tgcgcaccac catgacggcc acgcacgaga ccggccacat
     3541 gttcggcatc cggcactgca tcgcctacga atgcggcatg aatggttcga atcattctga
     3601 agagcgcgac cggcaaccgc tcgagttctg cccggaatgc cagcccaagc tctggtggac
     3661 ctgcgcgctc gacccgctcg agcgcagtcg cgcgctggcc gccgtcgccc gccggcatgg
     3721 atttcccaaa ctggctggcg cttttgatcg gcaaagccag gcgctcgcgt agttagcgag
     3781 gcagtccggc tttccataag acgaaatgtg gattcgcgca ggggaatccc cgcaggtatg
     3841 ctaccggccc atgaacccgc ccgatctgca gcagattcgc gccgccgtgg cccaggaccc
     3901 ccgcaacgcc gagctccggt atctgctggg cgccgagctc gcccagcaga aggaatacga
     3961 agccgcggtg gtggaaatga gcacggcgct gcagctcgat ccgctactgc atttcgcgcg
     4021 gtttcagctc ggtttgctct acctcaccat ggcgcagccg aatcagtcgc tggaaatctg
     4081 ggcgccgctc gagtcgctcg gtgaaaccgc ggcgctgaag tttttcaagc gcgggctcga
     4141 agcgttgatc cgggacgact tcgacacctg tgtcggcatg cttcggcatg gcatcgaact
     4201 caacacgcag aatgcgcccc tgaactacga catgacgcag gtcatcgatc gcgtggatga
     4261 agcgctggtg ccggcgcagt cgattaccgt ggcatccatg tccctggacg cgggtttttc
     4321 gcgctacgga aatcccggca agaccaggca ctgagttttc gccacggctc gcggccgtgg
     4381 cgtgtgaaat tactgcggca gtttctttcc gtcccacggg caaaaattcc agctcacgtg
     4441 cgccggcttg ccgttcgcgc acagacgcgg caccagaagc gaatcgatgg gacggtcgat
     4501 gctgaggctg atgtcctggc gggaggtgcg gccgctcaac ttcacgacga cagcgccggt
     4561 ctcgaacggc gagaacagcc gggcattctc cacctgatcc tggaatacga agaaatgaag
     4621 gttcctgccc aggttgccca tggcagcgcc catcgccgga acgagggttt tgagcagcgg
     4681 ttgcacatct tcgggcgcag gctcgagggg aacgaccttg cgagctttgc cggcagcatc
     4741 caccacttca acactgagcg atttggcgac cgcggcgcgg tcctggaagt cgactgccgc
     4801 tcctccgact tcgccgtcga ccacggcgat gaacgtgtat tg
//
LOCUS       DASQJS010000370         3502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3057506, whole genome shotgun sequence.
ACCESSION   DASQJS010000370 DASQJS010000000
VERSION     DASQJS010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3057506"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VEW08_14635"
     CDS             complement(<1..401)
                     /locus_tag="VEW08_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HYJ42027.1"
                     /translation="MKRGRVDVAVFPVAGRGTRFLPATKASPKEMLPIVDKPLIQYAV
                     EEALEAGARRLVFITGSSKRAIEDHFDTDGELENLLLKQGKSDLLKMLKSVLPSYASC
                     IYIRQPAPLGLGHAVLCAKPAVGNLPFYVHLA"
     gene            complement(557..895)
                     /locus_tag="VEW08_14640"
     CDS             complement(557..895)
                     /locus_tag="VEW08_14640"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HYJ42028.1"
                     /translation="MSYASLKRFIPTLAATLLLTQLVQAEPVNINTADATALAKALNG
                     VGPAKAKAIVSYREKNGPFKTVDQLAMVEGITQKLIDKNRSDIKLGADKAGGAQPAGQ
                     PAAKPAKPAS"
     gene            complement(978..1937)
                     /locus_tag="VEW08_14645"
     CDS             complement(978..1937)
                     /locus_tag="VEW08_14645"
                     /inference="COORDINATES: protein motif:HMM:NF037181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42029.1"
                     /translation="MRLRGIGPEVLLLCFLIIGAAAFALGRRSARGRRLRRDVDYFAG
                     LDHLVNDRFDRATEVFTRLATKSHEADIQFALGSLMRKRGEVDRAIAIHSELAASGEP
                     QIREQATFALGLDYLSAGLMDRAEEKLRSLLGSERYRPAVLERLAWVYEQQREWRAAL
                     ELWRELPAEKQLELAIVAAHYCCELGEVALAAGNLVEARAQVAAARVHAPNSARAALL
                     AARIATAAGESGKALELYVAALTCSRTIRIAFEPEARQALGAQAAALTEALHAQPAPD
                     EVEPPEPPRFRCEQCGVASGTWHWRCPSCRNWDSLRSTQNRGN"
     gene            complement(1956..3281)
                     /gene="aroA"
                     /locus_tag="VEW08_14650"
     CDS             complement(1956..3281)
                     /gene="aroA"
                     /locus_tag="VEW08_14650"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HYJ42030.1"
                     /translation="MSAAPVFPEFLDLEPWQQGSFHFETPLPGSKSLTLRDCAIAALA
                     DGQSTIRYPGECDDYWRMKDCLRKLGIAVDDSVEGVVRISGQGGQFDDGAIELNTGQS
                     AVSTRLLLAMAALRPGATLIDGHISMRNRPNKALVDALESLGAGMQSTNDGHLPTTVT
                     GTRNLRGPVRVPSNISSQYLTSLLIIAPLLDAGLVIEVEGELTSKPYVDLTLDEMTKF
                     GVRVINHDYRRLSVAPQVYRASDIDVEGDASAASYFAALATLHGARITLSNLGANTRQ
                     GDYAFFGLCEKLGARVERSTARTVIEGPRRLDAGFDSAIDMTSMPDVAPTLMMIAPFL
                     KSPTRITGLATLRVKECDRIAAPTRELRKLGVKVEEGPDYMVIEPLLDGTRPSVVEIE
                     TYHDHRIAMSFGVLGSRLPGLRILDPGCVAKTYPNYWQDWQRARSAPRL"
     gene            complement(3281..>3502)
                     /locus_tag="VEW08_14655"
     CDS             complement(3281..>3502)
                     /locus_tag="VEW08_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004244587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HYJ42031.1"
                     /translation="QILEIMSDALSAGDRIEIRGFGSFSLHFRPPRQGRNPKTGEAVA
                     LSGKYVPHFKPGKDLRERVNDGADRPIRD"
BASE COUNT          580 a         1122 c         1167 g          633 t
ORIGIN      
        1 gcaaggtgca cgtagaacgg cagattcccc actgcgggct tggcgcatag cacggcatga
       61 cccaacccca acggcgcggg ctggcggata tagatgcacg aggcatacga gggcaaaacg
      121 ctcttgagca tcttgagcag atcgctcttg ccctgcttca gcagcaggtt ttcgagctcg
      181 ccatcggtgt cgaagtgatc ttcgatggcg cgtttggacg aacccgtgat gaacaccagg
      241 cgccgcgcac cggcctccag cgcctcttcc accgcatatt gaatgagcgg cttgtcgacg
      301 atgggcaaca tctctttggg gctggccttg gtggccggga ggaagcgcgt tcctcttccg
      361 gcgacaggaa ataccgcaac gtcgactcgg cctctcttca ctgctcttcg ctcgacaatg
      421 gacatagtgg ctccggcccc atttgagggc cgaaaccata ccacgacacg attttcccct
      481 gtgcaggcaa ccgccccggg cgcgcgagag attgagcgac ttcacaactc tcgcggccac
      541 gaggaccggc cccgagctat gaggcgggct ttgccggctt ggcggcgggc tgcccggcag
      601 gctgcgcacc cccggccttg tccgcgccta gcttgatatc cgaccggttc ttgtcgatga
      661 gtttctgcgt gatgccctcg accatggcga gctgatcgac ggtcttgaaa ggaccgttct
      721 tttcgcggta gctcacgatg gctttcgcct tggcgggccc gacgccgttc agcgccttgg
      781 ccagcgccgt ggcatcggcg gtgttgatgt tgacgggttc ggcctgcacg agctgcgtga
      841 gcagcagcgt ggcggcgagc gtggggatga aacgtttcag ggatgcgtaa gacatgtggc
      901 ctccttgccg tgtgcccgat tgccgggtgg gaggagacta acaacgtcgg ggccgcgggc
      961 cgcagcggcc aatttgatca attgccgcgg ttttgcgtgc tgcgcaggga gtcccagttg
     1021 cggcagctcg ggcagcgcca atgccaggtg ccgctcgcca cgccacactg ctcgcagcgg
     1081 aagcgtggcg gctcaggcgg ctccacttca tcgggagccg gctgggcgtg cagcgcttcg
     1141 gtgagcgcag cggcctgcgc accgagggcc tgccgggctt cgggttcgaa cgcgatgcgg
     1201 atggtgcgcg aacacgtcag cgccgccacg tagagctcga gcgctttgcc ggactcgccc
     1261 gcggcagtgg cgatgcgtgc cgccagcaac gcggcgcgcg ccgaattcgg cgcatgtacc
     1321 cgtgcggccg ccacctgcgc gcgcgcttcg acgagattgc ccgccgccag cgccacttcg
     1381 ccgagctcgc aacagtagtg cgcggccacg atcgccagtt caagctgctt ttcggccggc
     1441 agttcgcgcc acagctcgag cgccgcacgc cattccctct gctgttcgta gacccaggcg
     1501 agccgttcga gcaccgccgg ccggtaccgc tccgatccca agaggctgcg cagcttttcc
     1561 tcggcgcgat ccatgaggcc cgcgctcagg tagtcgaggc cgagcgcgaa ggtggcctgc
     1621 tcgcgaatct gcggctcgcc gctggcggcg agttcactgt gaatggcgat ggcgcgatcg
     1681 acctcgccgc gtttgcgcat caggctgccg agcgcgaact ggatgtccgc ctcgtgcgac
     1741 ttcgtcgcga gccgcgtgaa cacttccgtc gcgcgatcga agcggtcgtt caccaggtga
     1801 tcgaggccgg cgaaatagtc gacgtcgcgg cgcagccgcc ggccgcgcgc tgaacggcgc
     1861 ccgagcgcaa acgcggccgc cccgatgatg aggaagcaca gcaggagaac ttcgggaccg
     1921 atgccgcgca gacgcatgcg gggaaattaa cagctttaaa ggcgcggagc gctccgggcg
     1981 cgctgccagt cctgccaata gttcgggtaa gtcttcgcca cgcagcccgg atcgagaatc
     2041 cgcaggcccg gaaggcgcga tcccagcacg ccgaaagaca tggcgatgcg gtgatcgtgg
     2101 taggtctcga tttccactac cgaaggtcgc gtcccgtcga gcagcggttc gatgaccatg
     2161 tagtccggcc cttcttcgac cttgacgccg agcttgcgca gttcgcgcgt gggcgcggcg
     2221 atgcggtcac attccttgac gcgcagcgtt gcgagaccgg tgatgcgtgt cggcgacttc
     2281 aggaacgggg cgatcatcat cagcgtggga gccacgtccg gcatgctggt catgtctatt
     2341 gcgctgtcga atcccgcatc cagcctgcgc ggcccttcga tgacggtgcg tgcggtggat
     2401 cgttctacgc gggcgccaag cttttcacag agcccgaaaa acgcgtaatc cccctggcgc
     2461 gtgttcgccc caaggttgct gagggtgatc cgcgcgccgt gcagcgtcgc cagagccgca
     2521 aagtacgaag ccgccgaagc gtcgccttcg acgtcgatgt cgctggcgcg atagacctgc
     2581 ggcgccactg acagccggcg gtagtcgtgg ttgatgacgc gcacgccgaa cttcgtcatt
     2641 tcatcgagcg tgagatcgac gtacggcttg ctggtgagtt ccccttcgac ttcgatcacc
     2701 aggcccgcgt cgagcaatgg cgcgatgatc agcaggctgg tgaggtactg gctggagatg
     2761 ttgctgggta cgcgcaccgg gccacgcagg ttgcgcgtgc cggtcacggt ggttggcagg
     2821 tggccatcat tggtggattg catgccggcg ccgaggcttt ccagcgcatc caccagcgcc
     2881 ttgttaggcc ggttgcgcat ggagatgtgc ccgtcgatca gcgtggctcc cgggcgcagc
     2941 gccgccatgg ccagcagcag gcgcgtcgag accgcgctct gcccggtatt gagctcgatt
     3001 gcgccgtcat cgaactggcc gccctgtccg ctgatgcgca ccactccttc gacggaatcg
     3061 tcgacggcga tgcccagctt gcgcaggcaa tctttcatgc gccagtagtc gtcgcattca
     3121 ccgggatacc ggatggtgct ctgaccgtcg gccagcgccg ctatggcgca gtcacgcagc
     3181 gtcagcgact tgctgccggg cagaggcgtt tcgaaatgaa aagagccctg ttgccagggc
     3241 tcgagatcca gaaattcagg aaaaaccgga gcagcgctca tcagtcccgg atgggccgat
     3301 cggcgccgtc gttgacgcgc tcgcgcaggt ctttgccggg cttgaagtgc ggaacgtact
     3361 tgccggacag cgccaccgct tcaccggtct tgggattgcg gccctgccgc ggcggccgga
     3421 agtgcagcga gaacgagccg aagccacgta tctcgatgcg atctcccgcc gacagggcat
     3481 cgctcatgat ctcgaggatc tg
//
LOCUS       DASQJS010000371         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3057899, whole genome shotgun sequence.
ACCESSION   DASQJS010000371 DASQJS010000000
VERSION     DASQJS010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3057899"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VEW08_14660"
     CDS             complement(<1..408)
                     /locus_tag="VEW08_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42032.1"
                     /translation="MIAVALALRTLLREWRSGELGVLVLAITVAVAALTGVGFLVNRI
                     GIAVDNQAGEVLAADLRLESGQVMDDKALDEARRRGLEVARSTGLFSVVFNGDTNQLT
                     SLRAVSEKYPLRGRVMLSDEPFGAPQQSNGIPAR"
     gene            complement(419..1081)
                     /locus_tag="VEW08_14665"
     CDS             complement(419..1081)
                     /locus_tag="VEW08_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003189864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ42033.1"
                     /translation="MSVLKAESLTKKVTSPEGMLTIVDDVSLDIAAGESVAIVGASGA
                     GKSTLLALLAGLDSPTSGRISLAGTDLTALDEDGRARLRAERVGFVFQSFHLVPSLTA
                     LENVMLPLELAGRRDARAAAAETLGRVGLEHRTGHYPRQLSGGEQQRVAIARAFVTRP
                     AVLFADEPTGNLDTHTGERIVSLLFDLNAQSHTTLVLVTHDRTVAARCGRILQMDSGM
                     LK"
     gene            1068..1715
                     /locus_tag="VEW08_14670"
     CDS             1068..1715
                     /locus_tag="VEW08_14670"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HYJ42034.1"
                     /translation="MSTLIRFIRLVLVFVWVWPAGAEGAAPRTVVVLGDSLSAGYGLK
                     VQEGWVTLLSQRLASEGYGYKVVNASVSGETTQGGLARLPRVLELHKPDIVILELGGN
                     DGLRGLPLATSRENLRRSIDLARAAKARVVLVGMLIPPNYGERYSSEFRDMFTTLASS
                     YSLTLVPFLLDRVALEPGLMQADGIHANARGQPQMLENVWPKLKPLLVAPRKPAS"
     gene            1744..>3090
                     /locus_tag="VEW08_14675"
     CDS             1744..>3090
                     /locus_tag="VEW08_14675"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYJ42035.1"
                     /translation="MEKHWLKSYPPGVRPEIDINEYASLKELIERGLAIHSVRDAYVQ
                     MGKAMTFGELDILSMNFGSFLQHSCGLKKGDRVAVMMPNTLQYPIAIHGALRAGFTVV
                     NTNPLYTAPELEHQLTDSGATVIVIVENFAHVLEKVIAHTPIKHVVVTSVGEMLGFPK
                     GNIVDFVVRHKRKQVKPFNLPGSKSFKQALKEGSKVRLQPVKLTHSDIAFLQYTGGTT
                     GVAKGAMLTHGNMVANVLQALEWIGPSFSRDPATLITALPLYHIFALTANCLLFVVLG
                     WRNVLITNPRDFPAFVAEMRKYKFTYLSGVNTLFNALLHTPGFDTLDFSSLRLTLGGG
                     MAVQEAVAKRWKEVTGKVLTQAWGLTETSPAACINLPGEDFNGSIGYPIPSTEISIRD
                     DDGNELGINVVGEICVRGPQVMAGYWNRPDETAKVMIGSDLLRTGDIGRIDERGLVF"
BASE COUNT          588 a         1031 c          934 g          537 t
ORIGIN      
        1 gcgcgcggga atcccgtttg actgctgggg cgcgccaaag ggctcatcgg aaagcatcac
       61 gcggccacga agcggatatt tttccgagac ggctcgcagc gacgtcagct ggttcgtgtc
      121 gccgttgaat accacgctga aaagacccgt actgcgcgcc acctcgaggc cgcgccggcg
      181 ggcttcatcg agggccttgt cgtccatcac ctggcccgat tccaggcgca gatcggccgc
      241 cagcacttca cccgcctggt tgtcgaccgc gatgccgatg cgattgacga gaaagcccac
      301 gcccgtgagt gcggcgaccg cgacggtgat cgccagcacc agcacgccga gctcgcccga
      361 ccgccactcg cgtagcaacg tgcgaagcgc gagcgccacg gcgatcatga atcgcccctc
      421 acttcagcat ccccgagtcc atctgcagga tgcgcccgca acgcgccgcc accgtgcgat
      481 cgtgcgtcac gagcaccagc gtcgtatgcg actgcgcgtt gagatcgaac agcagcgaaa
      541 cgatgcgctc ccccgtgtgc gtatccaggt tgcccgtcgg ttcgtcggca aacaacacgg
      601 cgggccgagt cacgaacgcc cgcgcgatgg ccactcgttg ctgctcaccg ccggagagtt
      661 ggcgcgggta gtggcccgta cgatgttcga gcccgacgcg ccccagcgtt tccgccgcgg
      721 ccgcacgcgc gtcgcgccgg cccgcgagtt cgagcggcag catcacgttt tcgagcgccg
      781 tcagtgaagg caccaagtga aaggactgga acacgaaccc gacccgttcg gcacgcagcc
      841 gcgcacgacc atcttcgtcg agcgccgtca agtcggtgcc ggcgagggag atgcgcccgg
      901 atgtgggcga atcgaggccc gccaggagcg ccaggagcgt ggatttgccc gcccccgatg
      961 caccgacaat ggcaactgat tcacccgcgg caatgtccaa cgaaacatcg tccacaatgg
     1021 tcagcatccc ttccgggctg gtaacctttt ttgtgaggct ttcggctttg agtacgctca
     1081 tcagattcat tcggttggtg ttggtcttcg tgtgggtctg gcccgccgga gccgaaggcg
     1141 ccgcgccccg caccgtcgtc gtgcttggcg acagtctcag cgccggatac ggcctcaagg
     1201 ttcaggaagg ctgggtgacg ctgctttcac agcggctcgc ctccgaaggg tacggataca
     1261 aggtggtcaa cgccagcgtc agcggcgaaa ccacgcaggg cggacttgcc cggctgccgc
     1321 gcgtgctcga gctgcacaag cccgacatcg tgatcctcga gctcggcggc aatgacggat
     1381 tgcgcggact gcccctcgcc accagtcgcg agaacctgcg ccgctcaatc gacctggcgc
     1441 gcgccgccaa ggcacgtgtg gtgctcgtag gaatgctgat tccgcctaac tacggcgagc
     1501 gctactcgag tgagtttcgc gatatgttca ccaccctcgc ctccagctat tccctgaccc
     1561 tggtgccgtt tctgctcgac cgggtggcgc tcgagcccgg cctcatgcag gccgacggca
     1621 tccatgcgaa cgccagggga cagccgcaga tgctcgaaaa cgtctggccc aagctcaagc
     1681 cgctgctagt tgctccgcgc aaacccgcga gctagcctgc gacaccgagt tcgaggggtg
     1741 gtcgtggaaa aacattggct caagtcgtat ccgccaggcg ttcgccccga gatcgacatc
     1801 aacgaatacg cttcgctcaa agagctcatc gagcgcgggc tcgccatcca ttccgtgcgc
     1861 gacgcgtacg tacagatggg caaggccatg accttcggcg agctcgacat cctgtcgatg
     1921 aatttcggct cgttcctgca acacagctgc ggcctcaaga agggcgatcg cgtcgccgtc
     1981 atgatgccta acaccctgca gtacccgatc gccatccacg gcgcgctgcg ggccgggttc
     2041 accgtcgtca ataccaatcc gctctacacc gcgccagagc tcgagcacca gctcaccgat
     2101 tccggcgcca ccgtcatcgt gatcgtcgag aacttcgcgc acgtgctgga gaaagtgatt
     2161 gcccacacgc ccatcaagca tgtggtggtc accagcgtcg gcgaaatgct tggattcccc
     2221 aaagggaaca tcgttgactt cgtcgtgcgc cacaaacgca agcaggtgaa gccgttcaac
     2281 cttcctggct ccaagagctt caaacaggcg ctcaaggagg gcagcaaggt ccgccttcag
     2341 ccggtcaagc tcacacactc ggacatcgcg ttcctgcagt acaccggcgg caccaccggt
     2401 gtcgccaagg gcgccatgct tacccacggc aacatggtgg cgaacgtgtt gcaggcgctc
     2461 gagtggatag gcccctcatt ctctcgagat ccggcgacgc tcatcaccgc gctgccgctg
     2521 tatcacatct tcgcgctcac cgcgaattgc ctgctgttcg tcgtgctcgg ctggcgcaat
     2581 gtgctcatca ccaatccgcg cgacttcccc gcgtttgtgg ccgagatgcg caagtacaag
     2641 ttcacgtacc tgagcggtgt gaacacctta ttcaacgcgt tgttgcacac acccggcttc
     2701 gacacgctcg acttcagcag tctgcgcctc acgttgggcg gcggcatggc cgtgcaggaa
     2761 gccgtcgcca agcgctggaa ggaagtcacc ggaaaagtcc tgacgcaggc gtggggactc
     2821 accgaaacat caccggccgc gtgcatcaac ttgccaggcg aggacttcaa cggatcgatc
     2881 ggatacccga ttccgtcgac cgaaatcagc attcgtgatg acgacggcaa tgaactcggc
     2941 atcaatgtgg ttggcgagat ctgcgtgcgc ggcccacaag tgatggccgg ttattggaat
     3001 cgccccgacg agaccgcgaa ggtgatgatc ggcagcgact tgctgcgcac cggggacatc
     3061 gggcgcatcg acgagcgcgg gctcgtgttc
//
LOCUS       DASQJS010000372         7014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4628537, whole genome shotgun sequence.
ACCESSION   DASQJS010000372 DASQJS010000000
VERSION     DASQJS010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4628537"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VEW08_14680"
     CDS             complement(<1..703)
                     /locus_tag="VEW08_14680"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HYJ42036.1"
                     /translation="MTSVSRVALVLSFLIAGVGHAADSPRPLRDDLGDTISVGTAPCR
                     IVSLAPGTTAMLFAAGAGHCVIGTIAHSEEPAEAGRVPVVGDAETLDFEQLIGLRPTV
                     VVVAVDVVQRVRIDRIRALGIPVYQVHVTRLAQMPDSLRRLGQLAGTEIAAKQRADEL
                     DTELAALGARYRNRAPIRVLYQIWDRPIYTIGGRHVITDALELCGAVNVFADLQTAAP
                     AVTREAAVLRNPQLIL"
     gene            complement(700..945)
                     /locus_tag="VEW08_14685"
     CDS             complement(700..945)
                     /locus_tag="VEW08_14685"
                     /inference="COORDINATES: protein motif:HMM:NF042692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6587 family protein"
                     /protein_id="HYJ42037.1"
                     /translation="MAQQILVAVIVAAAFAASAWKLAPARRRLRALLALDGWAARHPR
                     LAGWRERTLKPRIVRAAGTGCAGCAANVGVRPQHPPR"
     gene            complement(956..2830)
                     /locus_tag="VEW08_14690"
     CDS             complement(956..2830)
                     /locus_tag="VEW08_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HYJ42038.1"
                     /translation="MDAVVSTPSLRIALIGVPNCGKTALFNRLTGSRQKVANYPGVTV
                     ERREGERAALDGRVLRILDLPGTYSLSPTTLDEAITRDVLLGRVADAPAPELVVCVVD
                     AVHLPIGLRLALEVKRLGLPMVLALNRMDVAAKRGVHIDMARLATELGVPVLPTIAVK
                     RGGADALIEAIQPARWEHHRSIAAPVWQDATAEDAEQTQVDLRGLLAAVGYAAPKESH
                     WVARFDAILLHPVYGLLVLIAVMFVMFQAVFSWAEAPMDWIDGGMAWLGEFTAAHLPE
                     GPLSSLLVNGIIGGAGAVLVFLPQILILFLFILALEDSGYLPRAAYLLDRMMGGVGLS
                     GRAFIPLLSSFACAIPGIMAARTIPSWRDRLTTIMIAPLMTCSARLPVYALLIGALIP
                     AKAVGPFNLQGLVMFVLYVAGIVAALVVAWVMKHFAGVRQYSPLMLELPDYQWPHAGN
                     LLLGLWERVKIFLSRVGGIILALMIVLWFLSSYPAPPEGATGPAIQYSFAGKLGAMLE
                     YLFAPIGFNWQISIALVPGLAAREVAVGALGTVYSMSAAGDDVAGQLEPVIAATWSLA
                     TACSLLAWYVFAPQCLSTLAVVKRETNSWRYPLIMAGYLFALAYVASFITYRVVGSLA
                     "
     gene            complement(2857..3207)
                     /locus_tag="VEW08_14695"
     CDS             complement(2857..3207)
                     /locus_tag="VEW08_14695"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HYJ42039.1"
                     /translation="MTSPDSNSNSPHPLADLRRGEAAVVAGLAEVAGFEDGRGSGAVV
                     LGRLRDLGFVAGAHCEVIAKMWPVGDPMVVRIGGSTFALRRAEAAAVRVMRLAHKPAA
                     HAQLAPRDTSAALA"
     gene            3336..3974
                     /locus_tag="VEW08_14700"
     CDS             3336..3974
                     /locus_tag="VEW08_14700"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYJ42040.1"
                     /translation="MNYIAERRLEEKERRRAEIVDAAEAAGREVGLDALTMDDVARRA
                     RLSRALLYVYFQDRSDLMFGLAERAMTMLHARFVEAAERHRTGLEQVSAMGRAYVAFS
                     QEFPVLFDALARCELQSSEPTQGSPSEQACALGGDRLQASLVASIETGVRDGTIRADV
                     GSPMLMSVTLWGFMHGIIQLTTTKAHALTHHGVTAKALIDHAISMITRDLKS"
     gene            4068..5474
                     /locus_tag="VEW08_14705"
     CDS             4068..5474
                     /locus_tag="VEW08_14705"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYJ42041.1"
                     /translation="MAWAWAPGPTLAQGVPPDSSRPLAAVIDEYVREGLEANLSLRAQ
                     SLEVERSDAALAEARARYFPELALDARYTWAQGGRTFELPLGQALNPAYQTLNDLLIA
                     QGQQPQFPVVQDETIAFLREREQDTRITLRMPLLVPAIPAAVRAQRELLGASEYAHMA
                     LARQLKRDITVGYLHWLSAVRTQGIVDASVALLNENLRVNDSLHRNGKITQDQVLRAR
                     AELLAVTQQSREAQNLAAQSQSYLNFLLNRPLDTALENADVAAEVTARTRALADLRAA
                     ALADRPELAELTHLARASEAQVSIAKADRWPTLSLGADGGIQGEEYEFGRGSNYSTVS
                     LLFNWTFFDGGARRAAVRQASATARKTAIQLSELTQQVQLEVQQSLDRLNTSADSLAT
                     ADARAEAARAAFRIASRKRDEGAISQVEFIDARSSLTSAELNLNVTRFSVLARQAELD
                     YATAAGTLPLPHNSQVSP"
     gene            5471..6565
                     /locus_tag="VEW08_14710"
     CDS             5471..6565
                     /locus_tag="VEW08_14710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ42042.1"
                     /translation="MKTSRNVVALLLLAATLLACGKQDAPTEDRPTPVRIQHASAGPS
                     VPPIDTSGVVATKHEMRLSFKMGGVVRRIYVQEGDVVKQGQRLAEIELTEVNSQVEQA
                     RQMADKAERDLKRGENLYADQVISLEQLQDLRTQSAMAAAQYNSAQFNQGYSVISAPR
                     DGKVLRKLAEERELIPAGSPVLVFGESGRGYVVRAALADREIVNVKLGDKGEIRMDAF
                     PGQPMTGTIVEISSAADERTGMFPIEVQFDSPPPRLVSGLVTRLRLAPTTGTPLLTYI
                     PMSALVEGDGDRASVFVIDGAKKPAIALRREVRVAFITPDSIALESGLATGEAVITDG
                     ALFLENGETVEVLRDTTKQAVNTPKPATEG"
     gene            6584..>7014
                     /locus_tag="VEW08_14715"
     CDS             6584..>7014
                     /locus_tag="VEW08_14715"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYJ42043.1"
                     /translation="MSFLEFPIRRYQFTLVAFLCLVALGFFAFNKVPREEDPYFKIPG
                     YTIAAIYPGADPKDLERLVAKPIEDRLAALDDVMKMETSIRDGVSFTAIEFETFVDAD
                     KKYDEVTRELNALRPDFPPEVQFIVRRFSPGLVNTIQVALVS"
BASE COUNT         1308 a         2350 c         2301 g         1055 t
ORIGIN      
        1 cgagaatcag ctgcggattg cgcaacaccg ccgcctcgcg cgtgaccgcg ggcgccgcgg
       61 tctggagatc ggcgaagaca ttgacggcgc cgcacagctc gagcgcatcg gtgatcacgt
      121 gccggccacc gatcgtgtag ataggccggt cccagatctg gtaaagcacg cggatgggag
      181 cgcggttgcg ataacgcgcg ccgagcgccg cgagctcggt gtcgagttcg tctgcccttt
      241 gctttgccgc gatttcggtg ccggccagtt gacccagccg ccgcagcgaa tcgggcatct
      301 gcgcgagccg ggtcacgtgc acctggtaca cgggaatgcc gagcgcccgg atgcgatcga
      361 tgcgcacgcg ctgaacgacg tccaccgcca ccacgaccac ggtcggccgc agaccgatga
      421 gctgttcgaa atccagggtc tccgcgtcgc cgaccaccgg cactcggccg gcttcggcgg
      481 gctcttcgct gtgcgcgatg gttccgatta cgcagtgtcc cgcccccgcg gcaaacaaca
      541 tggctgtggt tcccggagcc agcgacacga tgcggcacgg cgcggtgccg acggagatcg
      601 tgtcgcccag atcgtcgcgc agtggccggg gggaatccgc cgcgtgacca acgcctgcga
      661 tcaggaacga aagaaccagc gcgactcgcg agaccgaggt caccgtggcg ggtgttgcgg
      721 ccgcacgccg acattcgcgg cgcagccggc gcaaccggtg cccgctgcgc gcacgatgcg
      781 tggtttcagg gttcgctcgc gccacccggc gagacgcgga tggcgcgcgg cccaaccgtc
      841 gagcgcgagc agcgcgcgca aacgccggcg cgccggcgcg agtttccacg cgctggccgc
      901 gaaggcggcg gcgacgatga cggcgacgag gatttgctgt gccatggcct cgaccctacg
      961 ccagagaacc gacgacgcga tacgtaatga acgaggctac gtatgccagc gcgaacagat
     1021 agcccgccat gatgagggga taacgccacg aattggtctc gcgcttcacc accgcgagcg
     1081 tggacaggca ctgcggcgcg aatacgtacc aggcgagcag cgaacaagcc gtggcgaggc
     1141 tccaggtggc cgcgatcact ggctcgagct ggccggcgac gtcgtcaccg gcggccgaca
     1201 tcgaatacac ggtgccgagc gcgcccaccg cgacttcgcg cgcggcgagg cccgggacca
     1261 gcgcgatcga aatctgccag ttgaaaccga tcggcgcgaa gaggtattcg agcatcgcgc
     1321 ccagttttcc ggcgaagctg tattgaatgg ccggcccggt ggcgccttcg ggcggcgccg
     1381 ggtagctgga caggaaccac aacacgatca tcaatgcgag gatgattccg ccgacgcggg
     1441 agaggaagat cttcacgcgc tcccacaggc ctaacaacag gttgccggca tgcggccact
     1501 ggtagtccgg cagctcgagc atcaacggcg aatactggcg cacgcccgcg aagtgtttca
     1561 tcacccaggc caccaccagc gcggcaacga taccggccac gtacagcacg aacatcacga
     1621 gcccttgcag gttgaacggc cccacggcct tcgccgggat cagcgcgccg atgagcagcg
     1681 cgtacaccgg cagccgcgcc gaacaggtca tcagcggcgc gatcatgatg gtggtcaaac
     1741 ggtcgcgcca gctcggaatg gtgcgcgcgg ccatgatgcc gggaatcgcg cacgcgaagc
     1801 tggacagaag cggaatgaag gcgcggcccg acagccccac gccacccatc atgcgatcga
     1861 gtagataggc cgcgcgcggc aggtagccgg aatcctcgag cgcgaggatg aacaggaaga
     1921 ggatcaggat ctgcggcagg aacacgagca ccgcaccggc gccgccgatg atgccgttga
     1981 ccagcaggct cgagagcgga ccctccggca ggtgcgcggc ggtgaactcg ccgagccagg
     2041 ccatgccgcc gtcgatccag tccatgggcg cttccgccca gctgaacacg gcctggaaca
     2101 tcacgaacat gaccgcgatc agcaccagga gcccgtagac tggatgcagc agaatggcgt
     2161 cgaagcgagc cacccagtgg ctctccttcg gtgcggcata acccaccgcc gcgagcagcc
     2221 cgcgcaggtc gacctgggtc tgttctgcat cttcggccgt ggcgtcctgc cacaccggtg
     2281 cggcaatgct gcggtggtgt tcccaacgag ccggctgaat ggcttcgatg agcgcatcgg
     2341 cgccgccgcg cttcaccgca atggtcggca gcacgggcac gccgagctcg gtggcgaggc
     2401 gcgccatgtc tatgtgcacg ccgcgcttcg cggcgacatc catgcgattg agcgccagca
     2461 ccatgggaag gccgaggcgt ttcacctcga gcgcgagccg cagtccgatc ggcagatgca
     2521 ccgcgtcgac cacgcacacc accagttccg gagccggcgc atccgccacc cggcccagca
     2581 acacgtcgcg cgtgatggcc tcatcgagcg tcgtcgggct caggctgtag gtgccaggca
     2641 gatcgaggat gcgcaggact cgcccgtcga gcgcggcccg ctcgccttcg cggcgttcga
     2701 cggtgacgcc agggtagtta gctaccttct gacggctgcc ggtcaggcga ttgaagagag
     2761 cggtcttgcc gcaattggga acgccgatca gcgcgatgcg cagtgacgga gtggaaacga
     2821 cggcgtccat gagagcggac ctttcggccg aaagcactaa gcgagtgcgg cgctggtgtc
     2881 cctgggcgcc agctgcgcgt gcgccgcggg cttgtgcgcc aggcgcatga ctcgcacggc
     2941 cgccgcttcg gcgcggcgca aggcgaatgt cgacccgccg atgcgaacca ccatgggatc
     3001 gccgaccggc cacattttcg cgatgacttc gcaatgcgcg ccggccacga agccgagatc
     3061 gcgcagccgg cccagcacca cggcaccgct gccgcgccca tcctcgaaac ccgcgacttc
     3121 ggcgagtccg gcgacgaccg cagcctctcc gcgccggagg tcggcgagcg ggtgggggga
     3181 attggagttg gaatctggcg aagtcacgcc ccgcagtatg ggtttggcca ctgcgaatgt
     3241 aaatgcgatg agttcgcatt tgagaacaga tgccgtttgc acacacctct actaattgac
     3301 atgccgtcag ggaaaggcct aggcttccgg cccatatgaa ttacatcgcc gaacgcagac
     3361 tcgaggaaaa ggagcgccgt cgcgcggaaa tcgtcgacgc cgcggaagcc gccggccgcg
     3421 aggtcggcct ggacgcgctc accatggacg acgtcgctcg ccgggcacgt ctctcgcgcg
     3481 cactcttata tgtgtacttc caggatcgct ccgatctcat gttcggtctc gccgaacgtg
     3541 ccatgaccat gctgcatgcc cgcttcgtcg aagccgccga acggcaccgc acggggctcg
     3601 aacaggtcag cgccatgggg cgcgcctatg tcgccttttc gcaggaattc ccggtgcttt
     3661 tcgacgcgct ggcacgttgc gaactgcagt cttccgaacc cacccagggc tcgccttcgg
     3721 aacaggcctg cgcgctcggc ggcgatcggc tgcaggcttc gctggtcgca tccatcgaga
     3781 ccggcgtgcg cgacggcacc atccgcgccg acgtgggttc gccgatgctg atgagcgtca
     3841 cgctgtgggg attcatgcac ggaatcatcc agctcaccac caccaaggca cacgcgctga
     3901 ctcaccacgg agtgaccgcg aaagccctca tcgatcacgc gatcagcatg atcacgcggg
     3961 atctcaagag ctgacttcaa ggacctcgtt acatggattg tttgcgcctc cgactagaca
     4021 tgcgtccagc gactacacaa ctgagagttg cggcagcctg tttggcgatg gcgtgggcat
     4081 gggcgcctgg ccccacgctt gcccagggag tgcctcccga ttcgagccgc cctctggcgg
     4141 cggtcatcga cgaatacgtg cgcgaagggc tcgaggcaaa tctcagcctg cgagcgcagt
     4201 cgctcgaggt cgaacgatcc gacgccgcgc tcgctgaagc gcgcgcgcga tatttccccg
     4261 aactcgcgct cgacgcgcgc tacacgtggg cgcagggcgg ccgcacgttc gaactgccgc
     4321 tggggcaggc gctgaacccg gcgtaccaga cgctcaacga cctgctgatt gcccaaggtc
     4381 agcaaccgca gtttcccgtg gttcaggacg aaaccatcgc attcctgcgc gaacgcgagc
     4441 aggacacgcg catcacgctg cggatgccgt tgctggtgcc ggcgattccc gccgccgtgc
     4501 gcgcccagcg cgagttgtta ggcgccagtg agtacgcgca catggcgctg gcgcgccagc
     4561 tcaagcgcga catcaccgtg ggctacctgc actggctctc ggcggttcgc acgcagggca
     4621 tcgtcgacgc gagcgtggcg ctgctcaacg agaacctgcg cgtcaacgac tccctgcatc
     4681 gcaacggcaa gatcacgcag gaccaggtgt tgcgcgcgcg cgcggaattg ctggccgtga
     4741 cgcagcagtc acgcgaggcg cagaacctcg cggcgcagtc gcagagttac ctcaatttcc
     4801 tgctcaaccg gccgctcgac accgcgttgg agaatgccga cgtggccgcc gaagtgacgg
     4861 cacgcacgcg tgcgctggcg gatttgcggg ccgcggcgct ggcggatcgt cccgagctcg
     4921 ccgaactcac gcacctcgcg cgcgccagcg aggcgcaggt gagtatcgcg aaagccgacc
     4981 gctggccgac cctgtcactg ggtgccgacg gcggcatcca gggcgaagaa tatgagttcg
     5041 gccgcggcag caattactcc acggtgtcgc tgctgttcaa ctggaccttc ttcgatggtg
     5101 gcgcgcgccg cgcggccgtg cgccaggctt cggccacggc gcgcaagacg gccattcaac
     5161 tcagtgaact cacgcagcag gtgcaactcg aggtccagca gtcgctcgac cggctcaaca
     5221 ccagcgcgga ttcgctggcc acggccgatg cgcgcgccga ggccgcgcgt gcggcgtttc
     5281 gcatcgcgag ccgcaagcgc gacgaaggcg cgatcagcca ggtggaattc atcgatgcgc
     5341 gctccagcct caccagcgcg gaactcaatc tcaatgtcac gcgcttctcg gtgctcgcgc
     5401 gccaggccga actcgattac gcgaccgccg cgggcacttt gccgctgccg cacaactcac
     5461 aggtatcgcc atgaagactt cccgaaacgt tgtcgcgctt ctgttgctcg ccgcaacgct
     5521 tctggcttgt ggcaaacagg atgcgcccac ggaggaccgg ccgacgccgg tgcgaatcca
     5581 gcacgcgagc gcaggacctt cggtgccacc gatcgacacc agcggcgtcg tggccacgaa
     5641 acacgagatg cgcctgtcgt tcaagatggg cggagtcgtg cgccgcatct acgtgcagga
     5701 aggcgacgtg gtgaagcagg gccagcgcct cgccgagatc gagctgaccg aagtcaattc
     5761 gcaggtcgaa caggcgcggc agatggcgga caaggccgag cgcgacctca agcgtggcga
     5821 aaatctctat gcagaccagg tcatcagcct cgaacagctc caggatctgc gcacccagtc
     5881 ggcgatggcc gccgcgcagt acaactccgc gcaattcaac cagggctact ccgtgatcag
     5941 cgcgccgcgc gacggcaagg tgctgcgcaa actcgccgag gaacgcgaac tgattcccgc
     6001 cggcagcccg gtgctggtat tcggcgagag cggacgcggc tacgtggtgc gcgcggcgct
     6061 cgccgatcgc gaaatcgtca atgtgaagct cggcgacaag ggcgagatcc gcatggacgc
     6121 attccccggc cagcccatga cgggaaccat cgtcgaaatc tcgagcgccg ccgacgagcg
     6181 caccggaatg tttcccatcg aggtgcaatt cgattcgccg cctccgcgcc tggtcagcgg
     6241 actcgtgacg aggctgcgcc tcgcgccgac gactggcaca ccgctgctca cctacatacc
     6301 gatgtccgca ctcgtcgaag gtgacggcga tcgggccagc gtgttcgtga tcgacggcgc
     6361 caaaaaacca gcgatagcgc tgcgccgcga agtgcgcgtg gccttcatca cccccgacag
     6421 cattgcgctg gagagcgggc tcgcgacggg tgaagcggtg atcaccgatg gcgcgctgtt
     6481 cctggagaac ggcgaaaccg tggaggttct gcgtgacacc accaagcagg cggtgaatac
     6541 accgaagccg gcgacggaag gctgagcggc aagggaggcg aacatgtcgt tcctcgagtt
     6601 tccgatacgc cgttaccagt tcacgctggt ggcgttcctg tgcctggtcg cgctcggctt
     6661 cttcgcgttc aacaaggtgc cgcgcgagga agatccctac ttcaagatcc cgggctacac
     6721 catcgcggcc atctacccgg gcgccgatcc caaggatctc gagcggctgg tggccaagcc
     6781 catcgaagac cgcctggctg cgctcgacga tgtcatgaag atggagacct cgatccgaga
     6841 cggcgtgtcg ttcaccgcca tcgaattcga gaccttcgtc gacgcggaca agaaatacga
     6901 cgaggtcacg cgcgagctga acgcgctgcg cccggacttc ccgcccgagg tgcaattcat
     6961 cgtgcgccgt ttctcgcccg gcctggtgaa caccatccag gtcgcactgg tctc
//
LOCUS       DASQJS010000373        20131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3379345, whole genome shotgun sequence.
ACCESSION   DASQJS010000373 DASQJS010000000
VERSION     DASQJS010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20131
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3379345"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2156
                     /locus_tag="VEW08_14720"
     CDS             <1..2156
                     /locus_tag="VEW08_14720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ42044.1"
                     /translation="LDKSLNHLLYIDAGPGNNTNTGLAATSAVAHITSMGTPYVVDTP
                     TGPRAYINEFSAENINTFDSLRSEPGSQGTWAIMPRAEFENDNWHATFDATVSRAKVV
                     SNQIELDIVQNPYRNLGSAGLNGITASVFTGGRDLAGYTATLFTPRAEHVPAGGYPIP
                     PAANAGTQAGAQMPGAPAGTAGDRFGVTGTNGQSRTDLDAGQFDVERLFDEESFLSGI
                     QVGARYEHQIYTSSGSRNTALGANTGAVTPSLSVQNPYAGNFFGGHAPGFTTNWRNLD
                     ILPTLALITPVNTDVRSVTNPNGLPPQFTGGPETGVFLTPYGLVNNYWDANYYNNNFS
                     NQNDVISGYVMAKADFELAGMRVRGNAGVRYEHTDNEIEALDCENCATALSTRPLPVN
                     HSMITRHYEQTYDYWLPSAMLSADLTEQLVLRMAYYKTYVRPQPRDSGPISSIQVPQA
                     LVPPVDPVYTIAIGATGLKPYTADSYDLSLEWYNRPGGLFAVAIYQKDVDDYIGPITD
                     QGVLCPANGIINGVDYGLGTLSIVGTNCFSSNTFVGAGGTPVNARVQVSGQTNQSPIR
                     VRGLELSVQQNFDFLPGFWSHFGGAANYSHTSIDGEDAAGNPITLPSVSEHNLNLIGY
                     YETSRFGARVVYNRRGEYDLAAGNSFVGDARQVMERSQVDASVSFNITDNISISLDGF
                     NLTDAMRAEFENDPMLPRRLDYDGRTYQATLRASF"
     gene            2158..3285
                     /locus_tag="VEW08_14725"
     CDS             2158..3285
                     /locus_tag="VEW08_14725"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HYJ42045.1"
                     /translation="MHLASAAALALVFCAATATAQIPDRKQVLDAAARVADWQLARLD
                     GTHITTHMNVESRDPRSWQQGAFWVGMTRLAQVSGEKRFIDAIHAQGERNAWKPGERV
                     HHADDHVIAQSYLWAARHGADKNVLTPTRGVFDAILADPPVVHLSFYFSRTYSEAQCL
                     RRWCWCDAIFMSPPAWLDLANQTGERKYRDYAMAEFWAATEFLYDPAEKLYFRDSRFF
                     ERRDEQGRKLFWSRGNGWVFAGIANMLELLPPNHADRPRLEALFKQMAARLKAVQKAD
                     GYWAPSLLGAENSPAETSGTGFFTYGLAWGVNRGLLPADDYRASVLNGWAALTRAVTR
                     DGRLGWVQQVSDRPEQVAEADTHFYGVGAFLLAASELAVLR"
     gene            3357..3896
                     /locus_tag="VEW08_14730"
     CDS             3357..3896
                     /locus_tag="VEW08_14730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ42046.1"
                     /translation="MDHSTKTTGPGEGNSERERFLKQLSERYRQPLTAYFQRRVSSRS
                     EAEDLTQEVFLRLVKRLDVETLDNPEAFVFTTAVNLLRDRFRRGQTQRSHLAEVSLQQ
                     PGIEELSPERVFDSRQHLESVIEALEQLDERCRDAFILHRLEGMKHAQIAELYGVSVS
                     SVEKYIIKALAVLTERQKG"
     gene            3896..4870
                     /locus_tag="VEW08_14735"
     CDS             3896..4870
                     /locus_tag="VEW08_14735"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HYJ42047.1"
                     /translation="MILQPESPNSLYEQAAAWVIRINGEPSEIDLDKFALWQAQGPQY
                     VRAYEDASRAWTAVGENSSAPKLLAMRREALERTGRYTRRWNRRGLAAGLVALIALPL
                     AILYNSSTRPPEQAFETGRGEQRVIVLADGSRMSLDAMTRVKVVYGHDERRVTLTSGR
                     ANFEVARDLARPMNVHAGPRTVTALGTVFTVEREPHDVVVTLVEGRVAVTSGNRARAL
                     IEMKPLQQLRIDDDGAITMRDGLDPVLAMAWREGKLIFDNEPLVDVVATMNNYVTTPI
                     VVEGSARELRVSGVFKAGDTPAFVEAMESLFPVEASRHPHSITLQMKN"
     gene            4948..7662
                     /locus_tag="VEW08_14740"
     CDS             4948..7662
                     /locus_tag="VEW08_14740"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ42048.1"
                     /translation="MKSAGRLHYRALVWTAVFAALIFVPSGTARAQNEVHEYNIPAGD
                     LGEALRAFAAASNTQILFAPEVVEGRRSHGISGRLSLEAAMTSLLANTSLTYRMTASN
                     VIIVMLEAETGVPPPASATPSSSVEAGEVPFVTLEEVIVTASKRLENVQVVPFSVLVV
                     THSAMERSNIRDFDDLVKIAPSVTITKTSQPGNNSINIRGIGTYAYSIATEPSVAVVI
                     DEIPQAFQAAAFAALVDVKQIEVLRGPQNTLFGKSASAGVISITTQPATDEFTGRADV
                     MTTDDGEHRVFATISGPVSEALKFRLAGNYSEYRGNIRNLSTGNWLNGNEDTTLRGKL
                     VWEPDEDWTITLSPFYTRTIGSCCAPAEYFVSPGSTTGGAATGPSRIPMSTFLAGITP
                     GPKNTLTRLDVDAQGNSEDYGAGLKIVRDIGDHTFTSITSYDSYRLDDRQDTDSTDID
                     FSAFQPISPAGGSANGGYFDIESKTQEFRLTSPEQRLRYVAGLFYSDTNSERYFVRGS
                     NTLDDYNISPTPSPTPANLPTTNSTAYSRYLATARATNYALFGQGNLGLTEKLDFLLG
                     LRVNREEIEYTFHDLGNNVTFGSPRCSTTAPSGTPIETCNHDTSVTGRTGFQYQFTPD
                     VMAFTTYSRGYKGMAYDLTSTLTTRTPVANGPMAGEPLADVIASNQPVPAETVDSYEL
                     GIKTSFFDHRLNWNLTAFYMVFEGFQAQSRDQNLNQNLLNSIGEVTSRGVETEFAALF
                     GNFSINAAGAWNEAIMEDFPNAGCFPRQTAAEGCVGNVQDLSGKPLFNAPEWKFNVNA
                     QYEFAFGERLAGFVNMGYRWQSAVVFNLLQDPDSVQEAYGIANLSGGFKAEHWKVTAF
                     VNNLFDESYALTRGRDAHINIPAGGNAVNWKPARDFARYYGLRASVFF"
     gene            7666..9504
                     /locus_tag="VEW08_14745"
     CDS             7666..9504
                     /locus_tag="VEW08_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42049.1"
                     /translation="MRLRIFLIAAALALGTTAAAGEIQAPSFNEVVEGRIQPKLERLV
                     MQLVKEGRGMQIDGTPVFNGSDKFLPGKIALAFSDLIESLPADDPRLARYIANFRSVA
                     KLTVDDPNDSWGIYYYLSALAALDRGGHLKEALDPLTLAKLRVRLDWRTFVDLDNYAL
                     IEHPNNYYCVAFAIARLRHQLGWEEGEGAKHLYAAMTSHYREYSGDYGFADETDGDGR
                     FDRYSVLLAGEIAHRFMETGDAPPAEVIDWLRKSTAVMLQRLNPRGEGFEYGRSLGPY
                     SETAIIEVLTAAALLNLLDDDDKTLAYAYASRAAQRYADFWLDARTGSVNLWDGGRRT
                     DKYRGKFRILGENFSLGHQYAYTNAAWNRMGFRNQPPLRDFAAKLERLPKRSTTWFSR
                     GKYDRMLLTLREGGRVIGLPLINGGVSQHMHVPYFPIPFSPGMLDGVADETEPFLLPR
                     FELEDGSVLMPIAYFRDVQVSANGHRTQVSYRQTELDRLGESAPKPDDRLAVTTTYVF
                     EPGVITRTDVYTARQPLNVKSVAMAFGSHSGSPVTSELTTRFGDGLVREFSVAGMDSC
                     RSASALDDIRYHTPQGALRSKVNCLRKAFTMREPVTIRWELRYRTH"
     gene            9509..10576
                     /locus_tag="VEW08_14750"
     CDS             9509..10576
                     /locus_tag="VEW08_14750"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYJ42050.1"
                     /translation="MTRPRSATHGLFTATINDVAARAGVSKRTVSRVMNEEPNITADT
                     RDRVLKVIERLGYRPNPAARRLASGKSHLIGLFHSVCTPPDAALVEQITFAMASERYR
                     VLICSSDHRSPNAAEELASLARIVPIDGAILCAPLSEQLTFAPAFHAQKIPCVRIGTS
                     AGLHCVSSDDREAARQMTRYLASLGHQRIGFVCGPREHDSMQQRRQGFFDGLQIAGLP
                     IDPCIILQGVDTFDSGVECGRRLLSDTAGRPTAIFASTDAMATGILRAAHEFGIPVPK
                     ALSVAGFGDQPVASQLWPALTTVRPPIRALADRAAAALLELLCRVSADRSQSLVPSAE
                     IILRDSTGPLGAQRVQLSIRN"
     gene            10745..13189
                     /locus_tag="VEW08_14755"
     CDS             10745..13189
                     /locus_tag="VEW08_14755"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ42051.1"
                     /translation="MDDSSIRCRNACVSLFIGLAFTCGAFAQTGEQDSNDEDRLLTTV
                     TVTAQKRDQDLQDVPITITAIGSQLLRDSGVRDIKDLTLLTSGLVVTSTSSEVSTTAR
                     IRGIGTVGDNIGLESSVGVVIDGVYRPRNGVGFGDLGELERIEVLKGPQGTVFGKNTS
                     AGVINVISKRPDFEGGASAELTAANFNGYEGAVSINGPLVDDKLAGRFYGAYRRRDGL
                     YDVSTGDGTRQTKEDANRDVVVGRGQLLFTPSENTSALFIADYARRTENCCGAVQIND
                     GATAPLMDAVSADSGVSRPADPFARLQFADRSDEQELRDMGLSAELTFEPSFGKITSI
                     TAWRNFESTIGQDADYSTGDVLWRNSKGPNGNEFKQLSEELRFSGETDKIEWMVGAFY
                     AGENLDSDLNLRYGADFETYYSLVLSAGMAPTLVNALTGRPAQTAYPTGAGYQDRFEQ
                     RSDSFALFTNETFHFTERLHGTIGLRYTNEQKDLDSSYTNSDNGAGCAASRARYPIIS
                     AALPAAAVPGWYNFGCATFADPIYNGVSTSQSIDESETSGTAKLAFDFSDQLMAFLSY
                     ARGYKASGFNLDRERTPIASTGAPLFAFDADTSFKPETVDSYELGAKYASSNHMFRLN
                     SSLFYQKFEDFQLNTFTGISFVVVSIPEVTSRGVDLDFDLAPTDGLVFNAGVTYAKTE
                     YGDFTPPFPALFRLPNAQMSFAPEWSASLATTYEHNLGSSMQWRFNVGAKYTSEYNTG
                     SDLNPVKLQEALTLVNARFGIGAQDQKWFLEAWAQNLTDEEYYQVVFDATLQTGTFDA
                     YLGAPRTYGLTLRMQF"
     gene            complement(13186..14853)
                     /locus_tag="VEW08_14760"
     CDS             complement(13186..14853)
                     /locus_tag="VEW08_14760"
                     /inference="COORDINATES: protein motif:HMM:NF006181.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HYJ42052.1"
                     /translation="MNASFFPRRLPYHLAVPETSLWYNLEVSATRFPSLPALLFGGGV
                     TNYREFRAQSEALAGFLQKKCGVRRGDRVALFMHNSPQFVIAYYGILRADAVVVPINC
                     MNTTGELEHLLQDAGATTIVTAQELQPRVEPLLETKGQGAAVQHSIVACYADYAGDEA
                     ATVLPEALAGPRMQFRQPQVTHWHEVLEQAHAPAPHTAGPDDVALICYTSGTTGKPKG
                     CTHRHRGVMHSTVAIAHWHDVRHHARALVVLPLFHVTGMQNSMNVHIYAGSSLVLMPR
                     WNREAAFTAIARHKVSTLTTVPAMIIDLISNYDAARHDLSSMRLIGGGGAAMPQAVAA
                     RLQELCGLTYIEGYGMTETLAPTHINPGDRPKPHCLGIPIFDTDARIIDPLTLAEVPQ
                     GAVGEIIVSGPQVLDEYWNKPGENAACFLMRDGRKFLRTGDLARVDEDGYFHFVDRLK
                     RMINAAGFKVWPAEVEALLYGHPGVQEACVIGKPDSRSGEMVKALVVPREAARATLAA
                     EELIAWSAERMAAYKVPREVEFVASLPKSATGKIDWRLLQEQEAARS"
     gene            complement(14856..15698)
                     /locus_tag="VEW08_14765"
     CDS             complement(14856..15698)
                     /locus_tag="VEW08_14765"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HYJ42053.1"
                     /translation="MPRKHKYKETPDNPQLRTLHRVRESGDRLFVAALARGLAILRCF
                     RSGDRYLGNQEIARRTGLAKPTVSRLTYTLKRIGFLTHSKAREEYALGAGVLSLGHTY
                     LAALNTRELARPFMLEMAEFAQATICLGENDGQHMVIVEICHGSPTYLLRLEVGQRVP
                     HNTTALGRAYLAAMTPEQREQRIEAITRPLPLAARAKLAAEMQSSLRNYDKHGFVYSF
                     GDWNPDVFAVGVPLISADRTRVMALSCSGAMFDVTRKRIVSELGPRMVMLRQRIFTAS
                     RGVF"
     gene            complement(15709..16929)
                     /locus_tag="VEW08_14770"
     CDS             complement(15709..16929)
                     /locus_tag="VEW08_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ42054.1"
                     /translation="MFEYSDKVQRLRSRLDEFMRAEIYPAEPIYAAQLADIRKCFPWD
                     SPPVMQELKKKARAAGFWNMFLPQSEHGAGLTNLEYAPLAELLGRSPIASEACNCSAP
                     DTGNMELLERYATPEHKKRWLEPLLAGEIRSAFAMTEPTVASSDATNIECRITRAGDS
                     YVLDGRKWWVSGAADARCKLLIVIGKTAPDNSNRHRQQSMILVPRDAPGVQVLRSLRV
                     FGYDDAPHGHAEVVFDKVRVPAEYLLGGEGEGFAIAQARLGPGRIHHCMRAIGLAERA
                     LSLLCRRTSARTTFGRKISEQTVTLERIASARIQIDSARLLVLNAAHLMDTVGNKAAA
                     KQIAMIKVATPAMACQVLDWAIQAHGGAGVCDDFPLAAGYAHARTLRLADGPDEVHSN
                     QIGRLELAQQLATA"
     gene            complement(16940..18007)
                     /locus_tag="VEW08_14775"
     CDS             complement(16940..18007)
                     /locus_tag="VEW08_14775"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HYJ42055.1"
                     /translation="MSSRTSIEPTATIPVRDAHVMDAARLASWLHERLPGAGALTIRQ
                     FSGGQSNPTYLLSTDSGRYVLRRKPPGVLLPSAHAVDREFRILTALAGSAVPVPRAIC
                     YCDDVSVAGTPFYLMEYVEGRIFWNPALPGLTPPERAAIYEEMNRVIAAIHAIDPDAA
                     GLADFGRRENYVARQIERWSKQYRASELEYIDSMEQLIDWLPRHMPPEEPARITHGDF
                     RIDNLIFDRHELRILAVIDWELATLGNAFSDFAYNCMPYRMPEAIRKGLAGLDLAQLG
                     IPSEQAYVGKYLARTGRSAESTSTFNFYIAFNLFRLASILQGVAARAKLGNASSEHAT
                     EAGSLTRPIADLGWAQARGVH"
     gene            complement(18016..18792)
                     /locus_tag="VEW08_14780"
     CDS             complement(18016..18792)
                     /locus_tag="VEW08_14780"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ42056.1"
                     /translation="MSVRAAFDLTGRTALVTGGSRGLGLAMAGALGEMGAKVIISARK
                     SDELDAAVASLQRDRGVSAVRVVGDLSRTENVNPFVDAVAAAAGAPIDILVNNAGATW
                     GAPASEHPLEGWNKVLGLNLTGVFLLTQQIGVRWMIPRKQGRIVNIASIAGIQGSQHL
                     QAIAYHTSKGGLVQFTRALAAEWGQYGISVNAICPGFIRTKFSQVVLDQLTPRVIEAT
                     PMKQLGEPADLEGLAVLLASGAARHITGQIIAVDGGASVV"
     gene            complement(18789..19943)
                     /locus_tag="VEW08_14785"
     CDS             complement(18789..19943)
                     /locus_tag="VEW08_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ42057.1"
                     /translation="MQFEFNEEQLALQESVRRFLTERYDFETRRNSLAKPTGSSDEIW
                     QAFAEQGFLSIGLPEAHGGIGGATELMLTMEEIGRALVLEPYLSTVALCAPLIADHGT
                     DSQQADLLPRVAAGGLKLALAHGEAEARYASTVRTEARRAGDSYVLNGAKATVPDGAV
                     ADLLLVSARMKDGAGKDLAVFLVDPNAPGVRLVRYRTQDGRSAADVFFDDVKLPAGEK
                     MLGVGDATAAIERAVQRGIAALCAEAVGAMEAANATTIEYTKSRKQFGQPIGRFQALQ
                     HRMADMYVQATQARSMSILATGRCAAQGMEKGDEALRRRDVSAAKCYVGKAARFVGQQ
                     AVQLHGGMGMADELAVSHYFKRLTMIDMTFGDSTHHLAVVSDSILLESHT"
     gene            complement(19947..20111)
                     /locus_tag="VEW08_14790"
     CDS             complement(19947..20111)
                     /locus_tag="VEW08_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42058.1"
                     /translation="MQFEFNEEQLALQESVRRFLTQRYDFETRRNSLAKPTGSNEIQR
                     NIIAHRLLGL"
BASE COUNT         3655 a         6472 c         6437 g         3567 t
ORIGIN      
        1 acctcgacaa gtcgctgaac cacctgctgt acatcgacgc gggaccgggc aacaacacca
       61 acaccggact tgccgccacc tcagcggtgg cgcacatcac cagcatggga acgccgtacg
      121 tggtcgacac tccgaccggt ccgcgcgcct acatcaacga gttctcggcc gagaacatca
      181 acaccttcga ctcgctccgt tccgagcctg gctcgcaggg cacctgggcc atcatgccgc
      241 gcgccgaatt cgagaacgac aactggcacg ccaccttcga tgccactgtg tcgcgcgcca
      301 aggtggtctc gaaccagatc gagctcgaca tcgtccagaa tccctatcgc aacctaggat
      361 cggccgggct caacggcatc accgcgtcgg tgttcaccgg cgggcgcgac ctggcgggat
      421 acaccgccac gctgttcacg ccgcgagcgg agcatgtgcc agcgggtggt tatccgattc
      481 ccccggccgc gaatgcggga acgcaggccg gcgcgcagat gcccggcgca ccggccggca
      541 cggccggcga ccgctttggc gtcaccggca ccaacggtca gagccgcacg gatctcgacg
      601 caggccagtt cgacgtcgag cgactcttcg acgaggagtc attcctgtcc ggtatccagg
      661 tcggcgcgcg ctacgagcat cagatctaca cctcgtcagg ttcgcgcaat acggcgctgg
      721 gcgccaacac gggcgcggtc acgccgagtc tctcggtcca gaacccgtac gccgggaatt
      781 tcttcggtgg ccacgcaccc ggtttcacca ccaactggcg caatctggac atcctgccga
      841 cgctggcgct gatcacaccg gtcaataccg acgtccgcag cgtcaccaac cccaatggac
      901 ttccgcccca gttcacgggc ggtcccgaga ccggcgtgtt cctcacgccc tacggcctgg
      961 tgaacaacta ctgggacgct aactactaca acaacaactt cagcaaccag aacgatgtga
     1021 tctcgggata cgtcatggcg aaggccgatt tcgaactcgc cgggatgcgg gttcgcggca
     1081 acgcgggcgt tcgctacgag cacaccgaca atgaaatcga agcgctggat tgcgaaaatt
     1141 gcgccacggc gctctcgacg cgaccactgc ccgtcaatca ttcgatgatc acgcgccatt
     1201 acgaacagac ctacgattac tggctgccgt ccgcgatgct gtccgccgac ctgaccgaac
     1261 agctggtgct gcgcatggcg tattacaaga cgtacgtgcg tccgcaaccg cgagactcgg
     1321 ggccgatcag ctccattcag gtgccgcagg ccctggttcc accggtggat cccgtttata
     1381 cgatcgcgat cggcgccacg ggcctcaagc cttacaccgc cgattcatat gacctgtcac
     1441 tcgaatggta caaccgcccc ggcggcctgt tcgccgtggc gatctatcag aaggatgtgg
     1501 atgactacat cgggccgatc accgatcaag gcgtgttgtg tcctgcgaac ggcatcatca
     1561 atggcgtcga ctacgggctc ggtacattgt cgatcgtcgg taccaactgc ttcagctcga
     1621 ataccttcgt gggggcggga ggaactccgg tgaatgcccg cgtgcaggtc tccggccaga
     1681 ccaaccagag cccgatccgg gtgagggggc tggagctctc ggttcagcag aacttcgatt
     1741 tcctgccagg cttctggagc cacttcggtg gcgctgctaa ctactcgcat accagcatcg
     1801 acggcgagga tgcggccggc aacccgatca cgctcccgag cgtgtccgag cacaacctga
     1861 acctcatcgg ttactacgag acctcgcggt tcggcgctcg cgtcgtctac aaccggcgtg
     1921 gggagtacga cctcgccgcc ggcaacagct tcgtcggcga tgcgcgccag gtcatggagc
     1981 gcagccaggt ggacgcgtcg gtctcgttca acatcaccga caacatctcc atctccctgg
     2041 acgggttcaa cctgaccgat gccatgcgcg ccgagttcga gaacgacccg atgctgccgc
     2101 gtcgcctcga ctacgacggg cgaacctacc aggcgacgtt gcgggcgagc ttctgacgtg
     2161 caccttgcca gcgcggcggc tctcgctctc gttttctgcg ccgcgacggc gacggcgcag
     2221 attccggatc gcaagcaggt actcgatgcc gccgcgcgcg tcgccgactg gcagctcgcg
     2281 cggctggatg gaacgcacat cacgacgcac atgaacgtgg agtcgcgcga ccctcgcagc
     2341 tggcagcagg gtgcattctg ggtgggaatg acccgcctgg cgcaagtcag tggtgagaag
     2401 cgcttcatcg atgcgattca cgcgcagggt gagcgcaatg cgtggaagcc cggcgagcgc
     2461 gtgcatcacg ccgatgatca cgtgatcgcg cagagttacc tgtgggcggc gcggcacggc
     2521 gcggacaaga acgtcctcac gccgacacgt ggcgtgtttg acgccatcct cgcggatccg
     2581 cctgtggtgc acctgagctt ctacttctcc aggacctaca gcgaagcaca gtgcctgcgc
     2641 cgctggtgct ggtgcgacgc aatcttcatg tcgccgcccg cctggctgga cctggccaac
     2701 cagaccggtg aacgcaagta ccgtgactac gccatggcgg agttctgggc agcaacggaa
     2761 ttcctgtacg acccggccga aaagctgtac ttccgcgaca gccggttctt cgagcgacgc
     2821 gacgaacaag gccgcaaatt gttctggagc cgcggcaatg gctgggtatt cgccggtatt
     2881 gccaacatgc tggagttgtt gccgcccaac catgcggatc gaccgcggct cgaagcacta
     2941 ttcaagcaga tggcggccag gctcaaggca gtccagaagg ccgatggcta ctgggcgccc
     3001 tcgttgttag gcgcggagaa ttcgccggcc gagaccagcg gaaccggctt cttcacctac
     3061 ggcctggcat ggggagtgaa ccgcggcctg ctgcccgcgg atgactatcg cgcgtccgtt
     3121 ctcaatggat gggccgcact gaccagggcc gtcaccaggg atggccgcct gggctgggtg
     3181 caacaggtca gcgatcgtcc tgagcaggtc gccgaggccg acacgcactt ctacggcgtg
     3241 ggcgcgttcc tgctggctgc cagtgaattg gcggtgctgc ggtaattcat tgaggattcg
     3301 ggtatgtagt gcgtcgtagt taagcgggct ttggtagcgc tcacggcgcg gagacgatgg
     3361 accactcgac gaaaacgaca ggcccgggcg agggcaatag cgaacgcgag cgtttcctga
     3421 agcagctctc ggaacgctat cgccagccgc tgaccgcgta cttccagcgt cgagtgagct
     3481 cgcgcagcga agccgaggat ctgacgcagg aggtgttcct gcgcctggtc aagcgtctcg
     3541 acgtcgagac gctcgacaat cccgaagcct tcgtcttcac gaccgcggtc aacctgcttc
     3601 gggaccgttt ccgtcgaggc cagacgcaac gcagtcatct cgcggaagtg agtctgcagc
     3661 aaccgggcat cgaggagctc tcgccggagc gcgtcttcga cagtcgccag catctcgaga
     3721 gcgtgatcga ggcgctggaa caactcgacg agcgatgtcg cgacgcattc atcctccatc
     3781 ggctggaagg catgaagcac gcgcagattg ccgagctgta cggggtatcg gtgagctcgg
     3841 tggaaaagta catcatcaag gcgctggcgg ttttgaccga gcgtcaaaaa gggtaatgat
     3901 tttgcagcca gaatctccaa attccttgta cgaacaggcc gccgcatggg tgatccggat
     3961 caatggcgag cccagtgaga tcgacctcga caagttcgcg ctgtggcagg cgcaagggcc
     4021 gcagtatgtc cgcgcgtatg aggacgcctc gcgtgcctgg accgcggttg gcgagaattc
     4081 ctcggcgccg aagttgctgg cgatgcggcg cgaggcgctg gagcgcaccg gtcgctatac
     4141 ccgccgctgg aaccggcgtg gtttggccgc cggcctggtt gcgttgatag ccctgccgct
     4201 ggccatcttg tacaactctt cgacgcggcc gccggaacag gcattcgaga ccgggcgcgg
     4261 cgaacagcga gtcatcgtgc tggccgacgg ttcgcggatg tccctcgatg cgatgacccg
     4321 cgtgaaggtt gtgtacggac acgacgagcg tcgcgtcacg ctgacgtcgg gccgcgcgaa
     4381 cttcgaagtc gccagggatc tggcgcggcc gatgaacgtg catgccgggc cgcgcaccgt
     4441 cacggcgctg ggtacggtgt tcaccgtcga acgcgaacca catgacgtgg tcgtcacctt
     4501 ggtcgaaggt cgggtggcgg tgaccagcgg caaccgggcg cgtgcactca tcgaaatgaa
     4561 accgctccag caactgcgca tcgacgacga cggcgcgatc acgatgcgcg atggcctcga
     4621 cccggtactg gcgatggcct ggcgcgaagg caagttgatc ttcgacaatg aaccgctcgt
     4681 cgacgtcgtc gcgaccatga acaactatgt gacgacgcct atcgtcgtcg aagggagtgc
     4741 gcgcgagctg cgcgtcagcg gcgtgttcaa ggcgggtgac actcccgcgt tcgtcgaagc
     4801 gatggagagc ctgttcccgg tggaggcgtc gcgccatccg cattcgatta cgctgcagat
     4861 gaagaattga ggacacgcgc agccggcgcg ttgtatgtgc agacccgctc gaacgggcat
     4921 gaagcacgct acgtcagagg gggatcgatg aagtccgcag gccggctgca ctaccgcgca
     4981 ctcgtctgga ccgccgtgtt cgcggcgttg atcttcgtgc catcgggaac tgccagggcg
     5041 caaaacgaag tccacgagta caacattccc gccggcgacc ttggggaagc gctgcgcgct
     5101 ttcgcagccg catcgaacac ccagatcctg tttgcgccgg aagtcgtcga gggtcgccgc
     5161 agccatggaa tcagcggccg actctcgctc gaagcggcga tgacctcgtt gctcgccaat
     5221 acctcactca cttaccggat gacggcgtcc aacgtcatca tcgtgatgct ggaagcggaa
     5281 accggtgtgc cgcctccggc gagcgcgaca ccctcgtcgt cggtagaagc cggggaggtg
     5341 ccgttcgtca cgctggaaga agtgatcgtc acggcaagca agcgtctcga gaacgtacag
     5401 gtagttccat tttccgtgct ggtggtgacc cactccgcga tggagcggtc gaacatccgc
     5461 gacttcgacg acctcgtgaa gatcgcacca tcggtgacga tcaccaagac ttcgcagccg
     5521 ggcaacaaca gcatcaacat ccgcggcatc gggacctatg cgtacagcat cgccaccgag
     5581 cccagcgtcg cggtggtgat cgacgagatt ccgcaggcct tccaggccgc cgcgttcgcc
     5641 gcgctggtcg acgtgaagca gatagaagtc ctgcgcggcc cgcaaaacac attgttcgga
     5701 aagtcggcgt ccgccggcgt gatcagcatc accacgcaac ccgccaccga tgagttcacg
     5761 ggccgtgccg atgtcatgac caccgatgat ggtgaacatc gcgtgttcgc cacgatctcc
     5821 ggccccgtct ccgaggcctt gaagttccgc ctggcgggaa actacagcga gtaccgcggc
     5881 aacatccgca acctcagcac cggcaactgg ctcaatggca atgaggacac gacgctgcgt
     5941 ggcaagctgg tgtgggagcc agacgaagac tggacgatca cgctgtcgcc gttctacacg
     6001 cggaccatcg ggtcctgttg cgcccccgcg gagtacttcg tgagccccgg gtcgacgacg
     6061 ggtggagcgg cgaccggccc gtcacgcatt cccatgtcca ccttcctggc cgggataacg
     6121 cccgggccaa aaaacacgct cacccgcctg gacgtggacg cgcagggcaa ctcggaggat
     6181 tacggcgcgg gtctcaagat cgtgcgcgat atcggtgacc acactttcac gtcgatcacg
     6241 tcatacgact cctaccggct ggacgatcgc caggatacgg actccacgga catcgatttc
     6301 tcggcattcc aaccgatctc gcctgcgggc ggctccgcga acggcggcta cttcgatatc
     6361 gaatcgaaga cccaggaatt ccggctgacc tcacccgagc agcgcctgcg ctatgtggcc
     6421 ggcctgttct acagcgacac gaattcggag cgctacttcg tgcgtggctc caacacgctg
     6481 gatgactaca acatctcgcc gacaccgtcg ccgacgcccg cgaatctgcc gaccacgaac
     6541 agcaccgcgt actcacgcta cctggcgacc gcgcgcgcga ccaactatgc gctgttcggc
     6601 cagggaaacc tcgggctcac ggagaagctc gatttcctgc tcggcctgcg cgtgaaccgc
     6661 gaggaaatcg agtacacgtt ccatgacctt ggcaacaacg tcaccttcgg cagcccgcgg
     6721 tgttcgacca ccgctccgtc cggcacaccg atcgaaacct gcaatcacga tacctcggtg
     6781 acgggtcgca ccggcttcca gtaccaattc acgccagacg tgatggcctt caccacgtac
     6841 tcgcgcggat acaagggcat ggcgtacgac ctcaccagca ccttgaccac gcgcacgccc
     6901 gtcgccaacg gacccatggc gggcgagccg ctcgcggatg tcatcgcctc caaccagccc
     6961 gtgcctgcgg aaaccgtgga cagctacgag cttggcatca agacgtcctt cttcgaccac
     7021 cggctcaact ggaacctcac cgccttctac atggtgttcg agggattcca ggcgcagagc
     7081 cgggaccaga acctgaatca gaacctgctg aacagcatcg gtgaagtcac gtcgcgcggc
     7141 gtcgagaccg agttcgccgc gctgttcggg aatttcagca tcaacgctgc cggcgcctgg
     7201 aacgaggcga tcatggagga cttcccgaat gcgggctgct tcccgcgcca gaccgccgcc
     7261 gaaggctgcg tcggcaacgt gcaggatctt tccggaaagc cgttgttcaa cgcgcccgaa
     7321 tggaaattca atgtcaacgc gcagtacgaa ttcgcgttcg gcgaacgtct cgccggcttc
     7381 gtcaacatgg ggtaccgctg gcaatccgcc gtggtcttca acctgctgca ggatccggat
     7441 tcggtgcagg aggcttatgg catcgccaac ctctccggcg gcttcaaggc cgagcattgg
     7501 aaggtgaccg cgttcgtcaa caacctgttc gacgagagtt atgcgctgac ccgggggcgc
     7561 gatgcgcaca tcaacatccc cgccggcggc aatgcggtga actggaaacc cgctcgcgac
     7621 ttcgcgcgct attacggcct gcgggcctcc gtcttttttt aagcaatgag actacgaata
     7681 tttctgattg ccgcggctct cgcgcttgga acgacggcag cagccggcga gatccaggca
     7741 ccgtccttca acgaagtcgt ggaggggcgc atccagccga aactcgagcg gctggtgatg
     7801 caactcgtga aagaggggcg ggggatgcaa atcgacggga cacccgtctt caatggctcg
     7861 gacaagttcc ttccgggcaa gattgcgctc gcgttctccg acctgatcga gtccctgccg
     7921 gccgacgatc cgcgtctcgc gcgatacatc gcgaactttc gcagcgttgc caaactgacg
     7981 gtggacgatc ccaacgacag ctggggcatt tactactatc tctcggcgct cgcggcgctc
     8041 gatcgtggcg gccacctgaa ggaggcgctc gatccgctga cgctggcgaa gctgcgcgta
     8101 cgactcgact ggcgcacatt cgttgatctc gacaactacg ctctcataga gcatcccaac
     8161 aactactact gcgtcgcctt cgcgatcgca cggctgcgcc atcagttggg ctgggaagag
     8221 ggcgaaggcg ccaaacacct gtacgcggcc atgacctctc actaccgcga gtactcaggc
     8281 gactacggct ttgccgatga gacggacggc gatggcagat tcgatcgcta cagcgtcctg
     8341 ctcgcgggag aaatcgcgca tcgattcatg gaaaccggcg atgcgccgcc tgccgaggtg
     8401 atcgactggt tgcgcaagtc cacggcggtc atgctccagc gcctgaaccc tcgcggcgaa
     8461 ggtttcgagt acggtcgaag cctgggtcct tacagcgaga cggccatcat cgaagtgttg
     8521 accgccgcgg cactcctgaa cctgctcgac gatgacgaca agacgttggc atacgcctat
     8581 gcatcgcgcg ctgcgcagcg atacgcggac ttctggctgg acgcgcgcac ggggtccgtg
     8641 aacctgtggg acggaggcag acgcaccgac aagtatcgcg gcaagtttcg catcctgggc
     8701 gagaacttct cgttgggaca ccagtacgcc tacaccaacg ccgcgtggaa tcgcatgggc
     8761 tttcgcaacc agcctccgct gcgggatttc gccgcgaagc tggaacggct gccgaaacgc
     8821 tccacgacct ggttctcccg tggcaagtac gaccgcatgc tgctcacctt gcgcgaaggc
     8881 ggtcgggtca tcggtttgcc gctgatcaat ggcggcgtta gtcagcacat gcatgttccg
     8941 tattttccga ttccgttctc gccgggaatg ctcgatggtg tcgccgacga aaccgagccc
     9001 ttcctgctgc cacgattcga actcgaagac ggctcggtgc tcatgccaat cgcgtacttc
     9061 cgcgacgtcc aggtcagcgc caacgggcac cgcacccagg tcagctatcg ccagacagag
     9121 ctggatcgcc tgggtgagtc ggcacccaaa cccgatgacc ggttggcggt gacgacgacc
     9181 tatgtcttcg aacccggggt gatcacgcga acggacgtgt acaccgccag gcagccgctg
     9241 aacgtgaaaa gcgtcgcgat ggccttcggc tcacattcgg gtagcccggt cacgagcgag
     9301 ctgacgacgc gattcggcga cgggctggtg cgcgaattca gcgtggccgg aatggactcc
     9361 tgtcgatccg catcggcgct cgacgacatt cgttatcaca cgccccaggg agcgttgcga
     9421 agcaaggtca actgcctgcg caaggctttc acgatgcggg agcccgtcac gatccggtgg
     9481 gaacttcgat accggacgca ctagctacat gaccagacct cgcagcgcca ctcacggact
     9541 tttcaccgcg acgataaatg acgtcgcggc gcgtgccggc gtctccaaac gcacggtcag
     9601 tcgcgtcatg aacgaagagc ccaacatcac cgcggacacg cgcgaccgcg tcctgaaggt
     9661 gatcgagcgg ctcggctatc ggccgaatcc ggccgcacgc cggctcgcga gtggaaaatc
     9721 acatctcatc gggctgtttc actcggtgtg cacgccaccc gatgcggcgc tggtggagca
     9781 gatcacgttc gcgatggcca gcgaacggta ccgggtgctc atctgttcga gtgaccatcg
     9841 ctcgccgaat gcggccgagg aactcgcatc gctggcgcga atcgttccca tcgacggcgc
     9901 gatcctttgc gcgccgctgt cggaacagct gactttcgct ccggccttcc atgcccagaa
     9961 gattccatgt gtccggatcg gaacgagcgc cggccttcac tgcgtttcgt ccgacgaccg
    10021 cgaagccgca cgccagatga cgcgatacct cgcgtcgctc ggccaccagc gcatcggctt
    10081 cgtctgcggc cccagggaac acgacagcat gcagcaacgc cggcagggat ttttcgacgg
    10141 cttgcagatc gcagggttgc ccatcgaccc ctgcatcatc ctgcaaggcg tggacacctt
    10201 cgactccggc gtggagtgcg gtcgccgact gctgagtgac acggcaggtc gtccaacggc
    10261 gatcttcgcg agcacggacg caatggcaac cggcattctg cgggccgcgc acgaattcgg
    10321 catcccggta cccaaggcgt tgtcagtggc gggattcggc gaccagcctg tcgccagcca
    10381 gctctggcca gcgcttacga ccgtgcgacc gccgattcga gctctggcag atcgcgccgc
    10441 tgcggcactg ctcgagctct tgtgccgtgt atccgcggac aggagccagt cgctcgtgcc
    10501 ctcggccgag atcatcctgc gcgactcgac gggcccgctg ggcgcgcagc gcgtacagct
    10561 ctccattcga aactagttag ctggtccggc cgcgcatccg cggcgccact cgggcttcat
    10621 tccgcacagc ggaatccaac gtgtattttg tcgcgtggtc gttgtcgccg ccgatgcgtt
    10681 gatgcaatct gtctcgcata gtgcggttcg atgtttcgca gtacgaaata cgggggattc
    10741 acaaatggac gacagttcca tccgctgccg caacgcgtgc gtttcactct tcatcggcct
    10801 tgccttcacg tgcggcgcat tcgcgcagac gggagaacaa gactcgaatg acgaagatcg
    10861 cctgctgacc acggtcaccg tcaccgcgca gaagcgcgac caggatctgc aggacgtgcc
    10921 gatcacgatc accgcaatcg ggtcgcagct gctgcgtgat tccggtgtgc gagacatcaa
    10981 ggacctgacg ttgttgacgt ccgggctcgt cgtcacctcg acgtcgagcg aggtttccac
    11041 gaccgcgcgt atccgcggaa taggcaccgt gggagacaac atcggccttg aatcttcggt
    11101 gggtgtcgtc atcgacggcg tctaccgccc gcgcaacggg gtgggcttcg gcgacctcgg
    11161 cgagctggag cgtatcgagg tgttgaaggg accacagggc acggtgttcg gcaagaacac
    11221 ctcggccggc gtcatcaacg tgatctcgaa gcggccggat ttcgagggcg gcgccagcgc
    11281 cgagctcacg gccgcgaact tcaacggcta cgaaggggcg gtttcgatca acggaccgct
    11341 cgtcgacgac aagctcgccg gcaggttcta cggcgcgtat cgacgtcgcg acggtctcta
    11401 cgacgtgagc accggtgacg gcacgcgcca gaccaaggaa gatgccaatc gcgatgtggt
    11461 agtcggacgg gggcagctgc tgttcacgcc gtccgagaac accagcgcgc tgttcatcgc
    11521 cgattacgcg cggcgcaccg agaactgctg cggcgccgtg cagatcaacg acggtgctac
    11581 tgcgccgctc atggatgcgg tctccgccga ctccggcgtg tcgcgtcccg cggacccatt
    11641 tgcccgactc cagtttgcgg atcgttcgga cgagcaggag ctgcgcgaca tgggcctgtc
    11701 cgcggagctc actttcgaac cttcgttcgg caagatcacc tcgatcaccg cgtggcgcaa
    11761 ttttgaatcg acaattggac aggacgccga ctacagcacg ggcgacgtgt tatggcgcaa
    11821 ttcgaaggga ccgaacggca acgagttcaa acaactgtcg gaggagctgc gtttctccgg
    11881 tgagaccgac aagatcgagt ggatggtcgg cgccttctac gccggggaga atctggattc
    11941 ggacctgaac ctgcgttatg gcgcagattt cgagacctac tacagcctcg tgctgtccgc
    12001 cggaatggcg ccaacgctgg tcaatgccct cacgggccgt cccgcgcaga cggcgtatcc
    12061 caccggcgcg ggttatcagg atcgcttcga gcagcgctcg gacagcttcg ccttgttcac
    12121 gaacgagacg ttccacttca cggaacgcct gcacggcacg attggcttgc gttacaccaa
    12181 cgagcagaag gacctcgatt cgtcgtacac caactccgac aacggcgcgg gatgtgcggc
    12241 ctcgcgcgcg cgttatccca tcatctcggc ggcactgccc gcggccgcgg tgccaggctg
    12301 gtacaacttt ggatgcgcga cattcgcgga tcccatctac aacggtgtca gcacgtcgca
    12361 gagcatcgac gaaagcgaga ccagcggcac ggccaagctg gcattcgact tcagcgacca
    12421 gctcatggcc ttcctttcct atgcgcgcgg ctacaaagcc agcggcttca acctggatcg
    12481 cgagcgcact cccatcgctt cgacgggcgc gcccttgttc gccttcgacg cggacacgag
    12541 cttcaagccc gagaccgtgg attcgtatga gctcggcgcg aagtacgcgt cttcgaacca
    12601 catgttccgg ttgaactctt cgctcttcta ccagaagttc gaggacttcc agctcaacac
    12661 cttcacgggc atcagcttcg tggtcgtgtc gattccggaa gtgacctcgc gcggcgtcga
    12721 cctggacttc gacctggcgc cgaccgacgg gctcgtgttc aacgccggcg tcacatacgc
    12781 gaagaccgaa tacggcgact tcacaccgcc gttcccggcg ctgttccggc tgccgaacgc
    12841 gcagatgtcg ttcgcgccgg agtggtccgc ctcgctcgcg accacctacg agcacaatct
    12901 cggcagctcg atgcagtggc gcttcaacgt cggtgcgaag tacacgtccg aatacaacac
    12961 gggctcggac ctgaaccccg tcaaactaca ggaagcgctt acgctcgtga acgcgcggtt
    13021 cggcatcggt gcgcaggacc agaaatggtt cctcgaggcc tgggcgcaga acctcacgga
    13081 cgaggagtac taccaggtgg ttttcgatgc gacgctgcag actggtacgt ttgacgccta
    13141 cctcggggcg ccgcgcacct acggcctgac attgcggatg cagttctagc tcctggcggc
    13201 ttcctgttcc tgcaggagcc gccagtcgat cttcccggtg gcggacttcg gcaggctcgc
    13261 cacgaattcc acttcgcgcg gcaccttgta cgcggccatg cgctcagccg accacgcgat
    13321 gagctcctcg gcggcgagcg tcgcgcgcgc cgcttcccgc ggcaccacga gcgctttgac
    13381 catctcaccg ctgcgcgagt cgggcttgcc gatgacacag gcctcctgca cgccaggatg
    13441 gccataaagc agcgcttcta cctcggcggg ccacaccttg aaacctgcgg cgttgatcat
    13501 gcgcttcagg cgatcgacga agtggaagta accatcttcg tcgacgcgcg cgaggtcgcc
    13561 tgtgcgcaaa aacttcctgc cgtcgcgcat gaggaagcag gctgcgttct ccccgggctt
    13621 gttccagtac tcgtcgagca cctgcggacc cgagacgatg atttccccca ccgcgccctg
    13681 cggcacttcg gcgagcgtca gcggatcgat gatgcgcgcg tcggtgtcga agatcggaat
    13741 gccgaggcaa tgcggcttgg gccggtcgcc cggattgatg tgagtcgggg ccagggtctc
    13801 tgtcatgcca tagccctcta tataagtgag gccgcagagc tcttgcagcc gcgcggccac
    13861 cgcctggggc atcgccgcgc ctccgccgcc gatgaggcgc atcgaggaca gatcgtgacg
    13921 cgccgcgtcg tagtttgaaa tgaggtcgat gatcatcgcg ggcacggtgg tgagggtcga
    13981 caccttgtga cgcgcgatcg cggtgaaagc ggcttcgcga ttccatcgcg gcatcagtac
    14041 caggctcgac ccggcataga tgtgcacgtt catcgagttc tgcatgccgg tgacgtggaa
    14101 cagcggcagc acgacgagcg cgcgcgcgtg gtgtcgaacg tcgtgccaat gcgcgatagc
    14161 cacggtgctg tgcatgacgc cgcggtgccg gtgcgtgcag cccttgggct tgccagtcgt
    14221 gccagaggtg tagcagatca acgccacatc gtcgggcccg gccgtgtgcg gtgccggtgc
    14281 gtgtgcctgc tcgagcactt cgtgccaatg agtgacctgc ggctgccgga attgcatgcg
    14341 cggcccggcc agcgcctcgg gcagcacggt ggccgcttca tcgcccgcgt agtctgcgta
    14401 gcaggccacg atggagtgtt gaaccgctgc gccctggccc tttgtttcga gaagaggttc
    14461 gacgcgcggc tgcagctcct gcgccgtgac gatcgtcgtg gcgccggcat cttgcagcag
    14521 gtgctcgagt tcgccggtcg tgttcatgca gttgatcggg acgaccacgg catcggcgcg
    14581 caggatgccg tagtacgcga tcacgaactg agggctgttg tgcatgaaca gcgcgacccg
    14641 gtcaccccgt cgcacgccgc acttcttctg caggaagccc gcgagcgcct cgctctgggc
    14701 acggaattcg cggtagttgg tcacgccacc gccgaacagc agggcgggga ggctcgggaa
    14761 gcgggttgcc gaaacttcga ggttgtacca gagcgaggtt tcgggaacgg cgagatggta
    14821 aggcaggcgg cgcgggaaga aactggcgtt catgctcaga atacgccgcg cgaggccgtg
    14881 aagatgcgct ggcgcagcat caccatgcgt ggcccgagct cactgacgat acgtttgcgc
    14941 gtgacgtcga acatcgcgcc gctgcacgac agcgccatga cgcgcgtgcg gtcagccgag
    15001 atcagcggca cgccgaccgc gaagacgtcc ggattccagt cgccgaacga gtagacgaag
    15061 ccgtgcttgt cgtagttgcg aaggctggat tgcatttcgg ccgccagctt ggcgcgggcc
    15121 gcgagcggca gaggccgcgt gatcgcctcg atacgctgct cgcgctgctc gggcgtcatg
    15181 gcagcgagat aggcccggcc cagcgccgtg gtgttgtgcg gcacgcgctg gccgacctcc
    15241 agccgaagta gataggttgg gctgccgtga cagatctcga cgatgaccat gtgctggccg
    15301 tcgttctcgc ccaggcagat ggtcgcctgc gcgaattccg ccatctccag catgaacggc
    15361 cgcgccagct cgcgcgtgtt gagcgcggcg agataggtgt ggcccagcga cagaacgccg
    15421 gcgcccagcg cgtattcttc gcgtgccttg gagtgcgtca ggaacccgat gcgcttgagt
    15481 gtgtatgtga ggcgcgagac tgtgggcttg gcgaggccgg tgcgccgcgc aatctcctgg
    15541 ttgccgagat aacggtcacc cgagcgaaag cagcgcagga tcgccagccc gcgcgccagc
    15601 gccgcgacga acaggcgatc cccggactcg cgcacgcgat gcaacgtgcg caactgcggg
    15661 ttgtcgggtg tttctttgta cttgtgcttg cggggcatga ggattctctt acgcggttgc
    15721 cagctgctgc gcgagctcga gtcgtccgat ctgattgctg tggacctcgt ccggtccatc
    15781 ggcgagacgc agcgtgcgcg cgtgcgcata acccgccgcg agcggaaaat catcgcagac
    15841 gccggcgccg ccatgagcct ggatggccca gtcgagcacc tggcaggcca tggcgggtgt
    15901 cgccaccttg atcatcgcga tctgcttggc ggcggccttg ttgccgacgg tgtccatcag
    15961 gtgagcggca ttgagcacca gcagccgcgc gctgtcgatc tggatgcgcg cactcgcgat
    16021 gcgttcgagc gtcacggtct gctcggagat cttgcggccg aacgtggtac gcgccgaggt
    16081 gcgccggcag agcagcgaga gcgcgcgctc ggcgaggccg atggctcgca tgcaatggtg
    16141 gatgcgaccc gggccgaggc gcgcctgcgc aatcgcgaag ccttcgcctt cgccgccgag
    16201 caggtattcg gccggcacgc gcactttgtc gaataccact tccgcgtgcc cgtgcggcgc
    16261 gtcgtcgtag ccgaacacgc gcaggctgcg cagcacctgc acgccaggcg cgtcgcgcgg
    16321 gacgaggatc atcgactgtt ggcgatgccg gttcgaattg tcgggagcgg tcttgccaat
    16381 gacgatgagc agcttgcagc gggcgtcggc ggcgccagag acccaccact tgcggccgtc
    16441 gaggacatac gagtctcccg cgcgagttat tcggcactcg atgttggtcg cgtcgctcga
    16501 ggcgacggtg ggctcggtca tggcgaatgc ggaacggatc tcgcccgcga gcagcggttc
    16561 gagccagcgc ttcttgtgtt cgggcgttgc gtagcgctcg agcagttcca tgttgccggt
    16621 gtcgggcgcg ctgcaattgc aggcctccga cgcgatcggc gaacggccca gcagctccgc
    16681 gagcggcgcg tattcgagat tggtgagccc ggcgccgtgc tccgattgcg gcaggaacat
    16741 gttccagaag ccggccgcgc gcgccttttt cttgagctcc tgcatgacgg gcgggctgtc
    16801 ccatggaaag cacttgcgga tgtccgcgag ctgcgccgca tagatcggtt cggccgggta
    16861 gatctcggcg cgcatgaact cgtcgagccg cgaacgcagc cgctgcactt tgtcggaata
    16921 ctcgaacata caggtccctc taatgaaccc cgcgggcctg cgcccagccg agatccgcga
    16981 ttgggcgggt gagactgccg gcttcggtgg cgtgctcgct cgatgcattg ccgagcttcg
    17041 cgcgcgccgc cacgccctgc aggatcgagg cgagccggaa tagattgaac gcaatgtaga
    17101 aattgaacgt tgacgtggac tcggctgaac gcccggtccg cgcgagatat ttcccgacgt
    17161 acgcctgttc ggacggaatg ccgagctgcg cgagatcgag accggcgaga cccttgcgaa
    17221 tggcctcggg catgcgatac ggcatgcagt tgtatgcgaa gtccgagaac gcattgccga
    17281 gtgtcgcaag ttcccagtcg atgaccgcga ggatgcggag ttcgtggcgg tcgaagatga
    17341 ggttgtcgat gcgaaagtcg ccgtgcgtga tgcgcgcggg ctcctcgggc ggcatgtgcc
    17401 gcggcagcca gtcgatgagc tgctccatgg aatcgatgta ctcgagctcg ctcgcgcgat
    17461 actgcttgct ccagcgctcg atctggcgcg ccacgtagtt ctcgcggcgg ccgaaatccg
    17521 cgaggccggc ggcgtcgggg tcgatcgcgt ggatcgccgc gatcacgcgg ttcatctctt
    17581 cgtaaatggc ggcgcgctcg ggcggcgtga ggcccggcag cgcggggttc cagaagattc
    17641 gcccctcgac gtactccatg agatagaacg gcgtgcccgc cacgctgaca tcgtcgcaat
    17701 agcagatggc gcgtggcaca gggacggcgc tgcccgcgag cgcggtgagg attcggaact
    17761 cgcggtcgac tgcgtgcgcc gacggcagaa gcacacccgg tggcttgcgc cgcaacacgt
    17821 aacggccgct gtctgtggat agtagatagg tcgggttcga ctggccgccg gagaattgcc
    17881 ggatcgtcaa cgctccggca cccggcaacc gctcgtggag ccagcttgcc agtcgcgccg
    17941 cgtccatgac gtgcgcatcg cgcaccggta tggtggcagt aggctcgatg ctcgtacgcg
    18001 aggacatgat ggcgctcaaa ctaccgaggc gccgccgtcg acggcgatga tctgcccggt
    18061 gatgtggcgc gcggcgccgc tcgcgagcag cacggcgagg ccttcgagat cggcaggttc
    18121 gccgagttgc ttcatcggcg tggcttcgat cacacgtggc gtgagttgat cgagcacgac
    18181 ctgagaaaac ttcgtgcgga tgaagcccgg gcagatcgca ttcacgctga tgccgtactg
    18241 gccccattct gcggccagcg cgcgcgtgaa ctgcacgagg ccgcccttgc tggtgtgata
    18301 agcgattgcc tgcaggtgct gcgagccctg gatgcccgcg atcgaggcga tgttgacgat
    18361 gcggccctgc ttgcgcggga tcatccagcg cacgcctatc tgctgcgtga gcaggaatac
    18421 acccgtgaga ttgagcccga gcaccttgtt ccatccctcg agcgggtgct cgctggcggg
    18481 cgcgccccac gtggcgccgg cgttgttgac gaggatgtcg atgggtgcgc cggccgccgc
    18541 agccaccgcg tcgacgaacg gattcacgtt ctcggtgcgc gacaggtcgc cgacgacacg
    18601 cacggcagat acgccgcgat cccgctgcag gctcgcgacc gccgcatcga gttcgtcgga
    18661 tttgcgcgcg gagatgatga ccttcgctcc catctcgccg agcgcgcccg ccatcgcgag
    18721 gccgaggccg cgcgaaccgc cagtgacgag cgccgtgcgt cccgtaaggt cgaatgctgc
    18781 gcgcacgctc atgtatggct ctccaggagg atcgagtcgc tgaccacagc gagatgatgc
    18841 gtcgagtcac cgaaggtcat gtcgatcatg gtcaggcgtt tgaagtagtg gctgaccgcc
    18901 agctcgtcgg ccatgcccat gccaccatgc agttggaccg cctgctggcc cacgaagcgc
    18961 gcggccttgc cgacgtagca tttggctgcg gacacatcgc gcctgcgcaa ggcctcatcc
    19021 cccttttcca ttccttgggc cgcgcatcgt cccgtggcca ggatgctcat cgagcgcgcc
    19081 tgggtggcct gcacgtacat gtcggccatg cggtgttgca gcgcctggaa gcggccgatg
    19141 ggctggccga attgcttgcg cgacttcgtg tattcgatgg tggtggcatt cgccgcctcc
    19201 atggcgccga cggcctcggc gcacagcgcc gcgatgccgc gctgcacggc acgctcgatt
    19261 gccgccgtcg catcgccgac accgagcatc ttctcgcccg cgggcagctt cacgtcatcg
    19321 aagaacacat cggcggcact gcgtccgtcc tgcgtgcggt agcgtacgag gcgcacaccg
    19381 ggagcattcg gatctaccag gaacaccgcg aggtctttcc cggccccatc tttcatccgg
    19441 gcactcacga gcaggaggtc ggccacggcg ccgtcgggca cggtggcctt tgcgccgttg
    19501 agcacatacg aatcgccagc gcgccgcgcc tcggtgcgca cggtcgacgc gtaccgcgcc
    19561 tcggcttcac cgtgcgccag cgcgagcttc aagccaccgg ccgcgacgcg cggcagcaga
    19621 tcggcctgct gcgagtcggt gccgtggtcc gcgatgagcg gcgcgcacag cgccacggtg
    19681 gatagatagg gctcgagcac cagcgcgcgg ccgatctcct ccatcgtgag catcagctcg
    19741 gtggcgccgc cgatgccgcc gtgcgcctcg ggcaggccga tggagagaaa accctgttcg
    19801 gcgaatgcct gccagatctc gtcgctcgag cccgtgggtt tcgcgagact gttgcggcgc
    19861 gtctcgaagt cgtagcgctc cgtgaggaag cgccgcacgg attcctgcag ggcaagttgt
    19921 tcttcgttga attcgaattg catttgtcag agccccagca ggcggtgcgc gatgatgttg
    19981 cgctggattt cgttcgagcc tgtgggtttc gcgagactgt tgcggcgcgt ctcgaagtcg
    20041 tagcgctgcg tgaggaagcg ccgcacggat tcctgcaggg caagttgttc ttcgttgaat
    20101 tcgaactgca tttgtcagag ccccagcagg c
//
LOCUS       DASQJS010000374         4617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_543283, whole genome shotgun sequence.
ACCESSION   DASQJS010000374 DASQJS010000000
VERSION     DASQJS010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543283"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VEW08_14795"
     CDS             complement(<1..583)
                     /locus_tag="VEW08_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HYJ42059.1"
                     /translation="MGKVIGIDLGTTNSCVAIMESGKPRVIENSEGDRTTPSIVAFTK
                     DNEVLVGQSAKRQAVTNPQNTLFAVKRLIGRKFDDAVVQKDINMVSYKIVKADNGDAW
                     VEAQGEKKAPPEISARVLMKMKKTAEDYLGEEVTEAVITVPAYFNDQQRQATKDAGRI
                     AGLDVKRIINEPTAAALAYGLDKAGKDRKIVVYD"
     gene            complement(657..1223)
                     /gene="grpE"
                     /locus_tag="VEW08_14800"
     CDS             complement(657..1223)
                     /gene="grpE"
                     /locus_tag="VEW08_14800"
                     /inference="COORDINATES: protein motif:HMM:NF010737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HYJ42060.1"
                     /translation="MPDPLNEHGTAGPDDKTTVLPETAVALVEMERLQQALAASEERA
                     KNHWEQYLRALADVENVRKRAAKDLESTRQFAVEKFAQDLIAVKDSLEMAIATFQKEG
                     AAKSDVASLIEGQNATLRQLAKAFEKAQIEEVNPEGAAFNPELHEAMMAQPSDAPPNT
                     VLSVVQRGYQLNGRLLRPARVVVAASKN"
     gene            complement(1376..2434)
                     /gene="hrcA"
                     /locus_tag="VEW08_14805"
     CDS             complement(1376..2434)
                     /gene="hrcA"
                     /locus_tag="VEW08_14805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HYJ42061.1"
                     /translation="MNETPDDALSEREQLLLRVLVGSYIRDGQPVGSRSLSRESGLAL
                     SSATIRNIVADLEQLGFVSSPHTSAGRVPTDKGYRFFVDSLLKSRAPIEDDPAFTELR
                     RQLDASRDDSKALVAAASQLLSNVTHLAGVVTLPFTRQASISQIEFVGLSDNRALVVL
                     VFNDREVQNRIIQLERYHSADELRRAANFLTERCRGKTLTQVRQDILGEMHEAREHMN
                     VAMKDAINVAEHVFKADEPNRMEYVMAGETNLMGAGELSSVDKLRRLFEAFNEKRDIL
                     NLLDHSLRADGVQIFIGQESGFRILDDISMVTAPYSADNEVVGVIGVIGPTRMAYERV
                     IPIVDVTAKILGAALARR"
     gene            2556..3449
                     /locus_tag="VEW08_14810"
     CDS             2556..3449
                     /locus_tag="VEW08_14810"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HYJ42062.1"
                     /translation="MPLPAQACALIGRFSDPRVAESALTLLPHLKKRGVRALLPESEA
                     LDAPADLVVRIPEDQIANQADLVIAIGGDGTLLYAARLVAHRGVPLVGINRGRLGFLT
                     DVLPQDMFASVDAALEGRAERDERALLEARIVSAKGITARALALNDVVLQKWETGRML
                     DFETWIDGAYVNTHGGDGLVVASSTGSTAYALSCGGPIVAPHLDVLVVAPICPHTLSD
                     RPIVVSGRSVITVKLIDRPDTRAQITCDGVSLGELTPGDRLEVHPTAQKITLLHPNKF
                     EYYRLLRSKLHWGRGGNNKSE"
     gene            3453..>4617
                     /locus_tag="VEW08_14815"
     CDS             3453..>4617
                     /locus_tag="VEW08_14815"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYJ42063.1"
                     /translation="MLTHLQIRDFAIIDLIDLEVRAGLTVLTGETGAGKSIVVDALAL
                     LAGGKGGAEVVRAGTERAELSATFDISESPAALREVLAEQSVDAEDELTVRRVIASDG
                     RSRGYLNGVSVPLQLLRDVGGLIVDIHGQHEFQSLTRSAAQRELLDDFGDNLELADKV
                     REAHKVWIALVNRTAELEGKARDRDSRLELLRFQVSELKALDLKEGEVAQLTEERTRH
                     SNRGRLAEAAQAAVGLLYDSDDGNAHATASRALAGIKTLSSVDQRLAKVIPMVDEATI
                     QIREAARELSRYLETLDIDPARQEEVERRLAAVEELSRKHRVPAAELAGRATTLENEL
                     AELDSADNNLNALKKAQTEALEGYRKLATQLSATRQTSARALSKDITARMQTLG"
BASE COUNT          824 a         1490 c         1425 g          878 t
ORIGIN      
        1 ggtcgtacac cacgatcttg cgatccttgc cggccttgtc gaggccgtag gcgagcgcag
       61 ccgcggtcgg ctcgttgatg atgcgcttca cgtcgagacc ggcgatgcgg ccggcatcct
      121 tcgtggcctg gcgctgctgg tcattgaagt atgccggcac cgtgatgacg gcctcggtca
      181 cttcttcgcc gagatagtcc tcggcggtct tcttcatctt catgagcacg cgcgcggaaa
      241 tttccggcgg cgccttcttt tcgccctggg cttcgaccca ggcatcgccg ttgtcggcct
      301 tcacgatctt gtaggacacc atgttgatgt ccttctgcac gacggcgtcg tcgaacttgc
      361 ggccgatgag gcgcttcacc gcgaacagcg tgttctgcgg attggtcact gcctggcgct
      421 tcgccgactg gcccacgagg acttcgttgt ccttggtgaa cgcgacgatg gacggcgtgg
      481 tgcggtcacc ctcgctgttt tcgatgacgc gcggcttgcc gctttccatg atggcgacgc
      541 aggaattcgt ggttcccaga tcgatgccga ttaccttgcc cataacgaaa tctccgaaat
      601 aactttttgt ttcagccagg ccccaaatgt ggtggccagg tccggtgttt caagaatcaa
      661 ttctttgagg ctgccaccac tacgcgggcc ggccgaagca atcgcccgtt cagctggtag
      721 ccgcgctgca cgacgctcaa cacggtattc ggcggggcgt cactgggctg cgccatcatg
      781 gcttcgtgca gctccggatt gaacgccgcg ccttccgggt tcacttcttc gatctgagct
      841 ttctcgaaag ccttcgccag ctggcgcaac gtggcgttct gcccttcgat caggctcgcg
      901 acgtcggact tcgccgcccc ctctttctga aaagtggcga tggccatctc gagggagtcc
      961 ttcacggcga tcaggtcctg ggcgaatttt tcgaccgcga actggcgggt gctctccaga
     1021 tccttggcgg cgcgcttgcg cacgttttca acgtcggcga gcgcgcgcag gtactgttcc
     1081 cagtgattct tcgcgcgctc ctcggaagcc gcgagcgcct gctgcagtcg ctccatttcc
     1141 accagcgcta ctgccgtttc cggcagaacc gtggtcttgt cgtcaggccc ggcggtaccg
     1201 tgctcgttca gcgggtcagg catcaaaaaa cctcaaataa aacaaatgat tgctggtgaa
     1261 attcaccagt catggagctg cgattgggcg ccgaggtggg gccggacggc ggattatcaa
     1321 ggattgcggt ggaaaatcct tcgcgccgca cggtccgaag ccgcgtgtcc tggcctcacc
     1381 ttcgggcgag agccgcgccg aggatcttgg ccgtgacgtc gacgatggga atgacccgtt
     1441 cgtaggccat ccgcgtcggc ccgatgacgc caatgacgcc gaccacttcg ttgtcggcgc
     1501 tgtacggcgc cgtgaccatg ctgatgtcgt cgaggatgcg gaatcccgac tcctggccga
     1561 tgaagatctg cacgccgtcg gcgcgcaggc tgtggtcgag cagattcagg atgtcgcgct
     1621 tttcgttgaa ggcttcaaag agacggcgca gcttgtcgac ggacgaaagc tcgcccgcac
     1681 ccatgagatt ggtttcgccg gccatcacgt attccatgcg gttcggttcg tccgccttga
     1741 acacgtgctc agccacattg atggcgtctt tcatggccac gttcatgtgt tcgcgcgcct
     1801 cgtgcatttc gcccaggatg tcctgacgaa cctgcgtcag cgtcttgccg cggcaacgct
     1861 ccgtgagaaa gttggcggcg cgacgcaatt cgtccgccga gtggtatcgc tcgagctgga
     1921 tgatgcggtt ctgcacttcg cgatcgttga agaccagcac caccagcgcg cgattgtcgg
     1981 aaagcccgac gaattcgatc tgcgagatcg atgcctggcg agtgaaaggc agcgtcacca
     2041 cgcccgccag gtgcgtgacg ttcgaaagca attgcgatgc ggccgcgacc agcgccttcg
     2101 aatcgtcgcg cgatgcgtcg agctggcggc gcagctcggt gaaagccggg tcgtcctcga
     2161 tgggcgcgcg cgacttcaac agtgagtcga cgaaaaagcg ataccccttg tcggtcggca
     2221 cacggccggc agaggtgtgc ggcgagctaa caaacccgag ctgctcgagg tcggcgacga
     2281 tgtttcggat ggtcgccgag gacagcgcca ggcctgactc gcgggacaag gaacgcgatc
     2341 cgaccggctg tccgtcgcgg atataactgc ctacgagcac ccgcagcagc agctgttcgc
     2401 gctcggaaag tgcatcgtct ggggtttcgt tcactgcgcc gaagctacct ttgtgggaat
     2461 caacgcgtca aggaaaccac gggtctgcgc cattcggcag ttttcgaagt gattcagtgc
     2521 attggccgcg tgtgtgtccc atgctacaaa gcagcatgcc tctaccggcg caagcttgcg
     2581 cgctaatagg ccgattcagc gacccgcgcg ttgccgagtc cgcgctgacc ctgctcccgc
     2641 atctcaagaa gcgcggggtg cgtgccctat tgccggaaag cgaagcgttg gacgcgcccg
     2701 ctgacctggt cgtacggatc cccgaagacc aaatcgccaa ccaggcggac ctggtcatcg
     2761 ccatcggcgg cgacggcaca ctgctgtatg ccgcgcggct cgtcgctcac cgcggcgtac
     2821 cgctggtggg catcaatcgc ggccgcctgg gttttctcac cgacgtgctc ccgcaggaca
     2881 tgttcgcttc ggtcgacgcg gcgctggaag gccgcgccga gcgtgacgaa cgtgcgctgc
     2941 tcgaggcgcg catcgtcagc gccaaaggca tcactgcccg cgcgctcgcg ctcaacgacg
     3001 tcgtgctgca gaagtgggaa accggccgca tgctggactt cgaaacgtgg atcgatggcg
     3061 cctatgtcaa tacccatggc ggggacggcc tggtcgttgc gagttccacc ggctccaccg
     3121 cctatgcact gtcctgtggc ggccccatcg ttgcaccgca cctcgacgtc ctggtggtgg
     3181 cgcccatttg cccgcacacg ctgtcagatc gcccgatcgt ggtgtccggg cgctcggtta
     3241 tcaccgtgaa actcatcgat cgccccgaca cccgcgcgca aattacctgt gacggtgttt
     3301 ccctgggcga actcaccccc ggggatcgcc tcgaagtgca cccgactgcg cagaagatca
     3361 cgcttctgca cccgaacaag ttcgagtatt accgcctgct gcgctccaag ctccactggg
     3421 ggcgcggtgg taacaacaaa tccgagtgag ccatgcttac ccatctgcag attcgtgact
     3481 ttgcaatcat cgatctcatc gatctcgagg tgcgcgccgg actcacggtg ctcaccggcg
     3541 aaacgggcgc cggcaagtcc atcgtggtcg atgctctggc gttgctcgcc ggcggcaaag
     3601 gcggcgccga agtggtgcgc gcggggaccg aacgcgccga actctcggcc actttcgaca
     3661 tcagtgagag tccggctgcg cttcgcgagg tgctcgcgga acagtcggtc gacgcggaag
     3721 acgaactcac ggtgcgccgg gtcatcgcca gcgatggccg ctcgcgcggt tatctgaatg
     3781 gcgtctcggt gccactgcag ctcctgcgcg acgtcggcgg cctcatcgtc gacatccacg
     3841 ggcagcatga attccagtca ctgacccgct ccgctgcgca gcgcgagctg ctcgacgatt
     3901 tcggcgacaa cctcgaactc gccgacaagg tgcgcgaagc acacaaagtc tggatcgcgc
     3961 tcgtgaatcg caccgccgag ctcgaaggca aggcacgtga tcgcgattcg cggctggaat
     4021 tgctccgctt ccaggtcagc gagctcaagg ccctcgacct gaaggaaggc gaagtggcgc
     4081 agctcaccga ggaacgcacc cgccattcca atcgcggccg gctcgccgaa gcggcgcagg
     4141 cggccgtcgg gctgctgtac gactccgacg atggcaacgc tcacgccacc gcctcgcgag
     4201 ccctcgctgg aatcaagaca ctctcgagcg tggaccagcg gctcgccaag gtgattccga
     4261 tggtggacga agccaccatc cagattcgcg aggcggcgcg ggaattgtca cgctacctgg
     4321 aaactctcga catcgatccc gcgcggcagg aagaagtcga acgccggctt gcagccgtcg
     4381 aagaactgag tcgcaagcac cgagtaccgg ccgccgaact cgccggccgc gccacgacgc
     4441 tggaaaacga actcgccgaa ctcgacagcg ctgacaacaa tctcaacgca ctcaagaaag
     4501 cccagaccga ggctctcgag ggctatcgca agctcgccac gcagctctcc gccacgcggc
     4561 agacttcggc gcgcgcgctc tcgaaggaca tcaccgctcg catgcagaca ctgggca
//
LOCUS       DASQJS010000375        11825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2744970, whole genome shotgun sequence.
ACCESSION   DASQJS010000375 DASQJS010000000
VERSION     DASQJS010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11825
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2744970"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(319..1470)
                     /locus_tag="VEW08_14820"
     CDS             complement(319..1470)
                     /locus_tag="VEW08_14820"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HYJ42064.1"
                     /translation="MATPGIVEPAFSLGLEEEYLLVDLATRDLVADPPAELLQRCIAE
                     TDGRVSPEFLRSQLEVKTSVCGSVAAMRRELAELRRTVCKVAAGYGIAPIAASTHPFA
                     RPVSRPPHTDHERYADIAREVQGGARRMIICGMHVHVGLDDDELRIDVMNQLSYFVPH
                     LLALSCSSPFWEGEDMGMQSFRLMLLSSLPRTGLPEHFESYGEFRRHLDALVRNGLIE
                     DTTKIWWDIRPSSRYPTLETRVFDCCTHLDDAVCLAALNLSLVRMLYRLRRENKVWRQ
                     YPRMLIAENRWRAMRFGSDATLLDLARGELVPFYELLEELLAITHDDAVALDCLAETE
                     HARVLIKRGTSAHQQVDVYQTARAQGATDGEALSAVVGWLARKTSEVGR"
     gene            complement(1505..5050)
                     /locus_tag="VEW08_14825"
     CDS             complement(1505..5050)
                     /locus_tag="VEW08_14825"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYJ42065.1"
                     /translation="MSDSSMRRLHPLLQSQLRELRARVTGGRVSAHELLEMLSRYYDS
                     IDDERRAMVRSMQLMSDEARSLGVEIAEQGAAQLQVILDHIKDVVITVDSAGVIDRAN
                     PMAERVFACASGELSGQNIDALVPGIALDGSIAAGLTRLTESSDTFRGMRQSPEVAAR
                     RRDGSPFPAEIAVSRARSGRGEVFVICLRDISERHSTQEALRDSEARYRSLVDHAPEA
                     ITVIDPDTQRFIEANEPALRLFKMTRDQLLAVHLDAVSAEMQPDGQPAMAPRQHTKLA
                     AAGESQVFEWIHRDSTGRDIPCEVRLARLQGGSKPLVRASITDISERKRAELIMENER
                     AFFALLASNAGLPAVLDVISALVQAVYPRCRCTISVLASDGTCFALTIARQLPPMLAA
                     VLERTPIEPRRGSCAAAVYSACDVFVSNVANDAHWADRRQVVLDAGFSAVWSMPIRGA
                     SGKLLGSVGVYRPEPGLPDQREHVLQSHATRLAALAIERNLAEEALRASEAKFRGLFE
                     GVIEGVYQSTRDGRLVSVNSAFVKMLGYSSAEEMYALPSSVMLYWSATDRADFVRRVD
                     SDGEVRSMEVVLRRRDGTQVVALENSRGVRDGSGRIVGFEGTVSDITERKRAEQAIFA
                     EKDRALVTLQSIGDAVISTDAGARIDYLNPVAERLTGWSVNEARGRPIGDVLQLIDES
                     TRQPVAYTLDRVLINGETSVPSDHIVLVNRRGEELAIQETATPIRNREGVAIGAVIVF
                     GDVTKERRLKRALSYQASHDALTGLINRREFDLRLETAVGAAQRGDGEHVLLYVDLDQ
                     FKVVNDTCGHSAGDRLLRDITSLLQTRVRASDTIARLGGDEFGLLLERCSLEQAERVA
                     DSIRQAIHGYRFLWGANSLSVGASIGVVRIARDTTSAASVLSAADIACYTAKDSGRNR
                     VQIYERDHGTNRHREMQWVGRIARAVEDGRLELYAQRIVGISANTGDTPFFELMVRLR
                     DEDGTLVPPGEFIPAAERYNVMVMVDRWVVGRAIELLAACIKQGRRLPLVAVNLSGTS
                     INDEDFLEFVLSRIHDEQVARALCFEITETAAVASLSKATYFMRELKARGCRFSLDDF
                     GSGVSSFVYLKTLPVDFLKIDGQFAANVAHDVVDRSMVEAIAKIGAAMQVATIAEKVE
                     SAEVLQVLKQIGVDYIQGFHLAEPCAIEDVFSCGN"
     gene            complement(5230..5796)
                     /locus_tag="VEW08_14830"
     CDS             complement(5230..5796)
                     /locus_tag="VEW08_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42066.1"
                     /translation="MLEIRHRAAFAALICACVLVACDDAPPEPQQGAPVVKKPDPPPK
                     EASISKDMVAAVSGGKSASVISVHFALREPPTVNKALAVDVAIVPHRDFTSVSAHFDS
                     QDGLTTTSGDFFGPKSDIDSETPLSHQLVLLPTREGMFMVTSSVDTVGVEGNITRIFS
                     IPIIVAAAAAPAPAGASATQPAREPDKN"
     gene            complement(5854..6849)
                     /locus_tag="VEW08_14835"
     CDS             complement(5854..6849)
                     /locus_tag="VEW08_14835"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HYJ42067.1"
                     /translation="MAVTHSAPAPFDALDPGAVFAAAESIGLQPSGRLFALNSYENRV
                     YQLGDDDGALWVLKFYRPARWSDAQIGEEHAFTLEMAAAELPVAAPIERDGESLFEHQ
                     RLRFAAFPYLAGRAPELDDKATLTLLGRTLARMHAIGSRAPFRHRPALTIERLGVHAR
                     STVQRSGFVPEALEEQYARVSEQVIGRVRQCLENFGPLPGLRIHGDCHAGNILWRENG
                     PLFVDLDDCMSGPRIQDLWMFLSGDAASQQSSWAAIMEGYELFGEFDFAELTLVEALR
                     SLRILHHAAWIASRWNDPAFPRAFPWFGDARFWERHIADLFEQLSAMDDPPILSR"
     gene            6919..7914
                     /gene="galE"
                     /locus_tag="VEW08_14840"
     CDS             6919..7914
                     /gene="galE"
                     /locus_tag="VEW08_14840"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HYJ42068.1"
                     /translation="MPSNSTVLVTGGAGYIGSHTVLQLIARGERVVVLDDLSTGFRQA
                     VRDVPLVVGNVGDRRLVDSVLAEHRVDTIVHFAAHTIVPESVSNPLKYYGNNTCATRS
                     LLEAASQAGVKNFVFSSTAAVYGIPATGHASEDSPTAPINAYGTSKLMSEWMLRDLCA
                     ATPMRHVVLRYFNVAGSDPQGRIGQSTRAATLLVKVACEAAVGKRPYLSVFGTDYPTP
                     DGTGVRDYIHVEDLASAHLNSLDYLRAGGNSLVANVGYGHGYSVREVLSSVEKIAGLK
                     LDVREEPRRAGDPPSLVARCDRVRELLKWQPRLDDIDTIVRTSLEWEKRLQREPW"
     gene            7938..9404
                     /locus_tag="VEW08_14845"
     CDS             7938..9404
                     /locus_tag="VEW08_14845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYJ42069.1"
                     /translation="MRLPRPKSLTGLMLLGLALIAGPLLVAVVDAAIQIRSLAQSSQE
                     LVKEGVNSARLSQSLFADITSLDRTVQLYQVIGKVEMLKSIRDTDQRLATTRTELAKL
                     LTEEPTRQSLEDFAAMHNEISSAVGVTPPGSPAFATILTRIDRLTDLANAIAASGNTQ
                     IDARLAEMQRQTSSTQKRLFWQSALLAPLTLIAILALTLSIGRPLRAIDRAISELGRG
                     TFSRSIEVQGPRDLERLGAQLEWLRLRLLDLAQERNRFLRHMSHELKTPLANIREGAE
                     LLMDGAVGPLDNSQREVVAILRDNGIKLHRLIENLLSFSAWQKESVGLDLSEFRLRPL
                     VKQVLESQQLTVVSQRVRLEVKVDDLMLRADRGKVRLIIENLLSNAIKYSPKGGTVYL
                     HAAKQDTQLMIDVADAGSGIPSEERARVFEAFYTGRPPAGVQVKGTGIGLSVVLEFVN
                     AHGGTIEIVDGEFPGAHFRIRMPVLSVTHPVRTQAHAA"
     gene            9394..10023
                     /locus_tag="VEW08_14850"
     CDS             9394..10023
                     /locus_tag="VEW08_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42070.1"
                     /translation="MRHKPLLACISCCALLTAGCGSGLPIKRDTQALPSIDRNSTEAS
                     LVAGYLEICMKAARGTPAEQAEVLATTHNDYAAAPTPSRVLRYAMVLSTPGHAGSDPV
                     VAQRLLREVLASPETLLPAERSLASLQLQQVERQQSLQADVRRLQQAAERNANEKIAQ
                     LTRSLKAEGDEVNRLKKALAEAQAKLDAIANIEGSSNNRRTPTPEGRKP"
     gene            10045..11403
                     /locus_tag="VEW08_14855"
     CDS             10045..11403
                     /locus_tag="VEW08_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013358747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYJ42071.1"
                     /translation="MQLATPHSTKRKARILVVDDDPGLLRLLTIRLRAENYEVEAVES
                     AALALAATSRFRPELVITDLRMDQMDGIGLLKELQSRYPGLKVIILTAHGTIPDAVHA
                     TQLGAFGFLTKPVDKQELLDQVQKALRISGFADNDEDWRAEIITRSSLVEEKLAQAHM
                     VAGTDARVLITGESGTGKELLARAIHNASPRRAKPFVAINCSAMAENLLESELFGHIK
                     GSFTGAVRDHAGLFQAADAGTLLLDEIGDMPMRLQVKLLRVLQENNIRPIGSTDAIPV
                     NVRVISATHRDLQQLMMSGQFREDLYYRLNVVHIEMPPLNRRREDIPLLVSHFLAQIS
                     NESGVRKIYAPEAVELLATADWPGNIRQLSNVVRQNVALSQTPIIPVELVQQSLGGTQ
                     SKLPSFDEARDEFTRSYLSQILQITGGNVSQAARLAKRNRTDFYKLLSRHQLTPEDFK
                     GR"
     gene            complement(11390..11617)
                     /locus_tag="VEW08_14860"
     CDS             complement(11390..11617)
                     /locus_tag="VEW08_14860"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYJ42072.1"
                     /translation="MALEQTSTSVAPGVRDCAAGHGWLPTWNSGLPSALRIRIDQCLV
                     SGAVSVADVHVRESVGSDHYATINDLSINAP"
BASE COUNT         2178 a         3948 c         3719 g         1980 t
ORIGIN      
        1 cgttgttggc cagcgcggcg cttgtcttga tagcgcggac gcgctgacca ctaggaaatg
       61 gggacattcc tcgtttccta gcaaaagggg acggggctcg ttggtggagg gtcgagcgac
      121 gggcggccct gcaagcgatg agactcgctt ttcgccgggc cctgccgccc gactcaactg
      181 accacaagat gtcttcgcgt gtgtcggcgg cccgcaaact agcgatgcca caccgaacga
      241 agggcccggc gaaaagcgaa cgaaatcctg tgcgcggact gctcgctggg gcacacccgc
      301 cctcacgccg cgccccgttt acctgccaac ttcgctggtc ttgcgagcga gccagccaac
      361 cacggccgag agcgcctcgc cgtcggttgc gccctgcgcg cgcgcagtct ggtaaacgtc
      421 tacctgctgg tgcgcgctgg tcccgcgctt gatcagcacg cgcgcatgtt ccgtctccgc
      481 gaggcaatcg agcgccacgg catcgtcatg cgtgatggcc agcagttcct cgagcaactc
      541 gtaaaagggc accagctcac cgcgcgccag atcgagcagc gtcgcgtcgc tgccgaatcg
      601 catggcgcgc cagcggtttt cggcgatcag catgcgtggg tactgccgcc agaccttgtt
      661 ctcgcggcgc agacgataaa gcatgcgcac cagcgagaga ttgagcgcgg ccaggcacac
      721 cgcgtcgtcg aggtgcgtac agcaatcgaa cacccgggtt tcgagcgtgg ggtagcgcga
      781 gctcggccgg atgtcccacc agatcttggt ggtgtcctcg atgagcccgt tgcgaaccag
      841 cgcgtccagg tggcggcgga actcgccgta actctcgaaa tgttcaggca atccagtgcg
      901 cggcaacgag gacagaagca tgagacggaa cgactgcatg cccatgtctt cgccttccca
      961 gaaaggcgaa gaacaagaca acgccagcag gtgcggcacg aagtagctga gctgattcat
     1021 cacatcgatg cgtagctcgt cgtcatcgag gccgacgtgc acgtgcattc cgcagatgat
     1081 catgcgccgt gccccgccct gcacttcacg cgcgatgtct gcataacgtt catggtcggt
     1141 atgcggcggc cggctcacag gccgcgcgaa cgggtgtgtg gacgcggcga tgggcgcaat
     1201 gccgtagccg gccgcgacct tgcacaccgt gcggcgcaac tctgccagtt cccggcgcat
     1261 ggcggcgacc gagccgcaca ccgaagtctt cacttcgagt tgcgaacgca ggaattccgg
     1321 gctgacgcgc ccatcggtct cggctatgca ccgctgcagg agttcggcgg gcgggtcggc
     1381 gacgagatcg cgcgtggcga gatccacgag caaatattct tcttccagcc ccaaagagaa
     1441 ggctggctca acgatccccg gcgttgccat gaacaggaac actacacggg ttttgtgagg
     1501 cgcgtcagtt cccgcaggag aaaacgtctt cgatcgcgca gggctcggcc aggtggaaac
     1561 cctggatgta atcgacaccg atctgcttga gcacctggag cacttcggcg ctttccactt
     1621 tttccgcgat cgtcgccacc tgcatggcgg cgccgatctt ggcgatcgcc tcgaccatgc
     1681 tgcgatccac gacgtcgtgg gcgacgttcg ccgcgaactg cccgtcgatc ttgaggaaat
     1741 ccaccggcag ggtcttcagg tacacgaacg aagacacgcc gctgccgaag tcgtcgagcg
     1801 agaaacggca gccgcgcgcc ttgagctcgc gcatgaaata cgtggctttg gacaggctgg
     1861 cgaccgccgc cgtctccgtg atctcgaaac acaacgcacg cgccacctgt tcatcgtgta
     1921 ttcgcgacag cacgaactcg aggaaatcct cgtcattgat ggacgtgccc gacaggttga
     1981 cggccaccag cggcagacgg cggccctgtt ttatgcacgc cgccagcagc tcgatggcgc
     2041 ggccgaccac ccagcgatcc accatgacca tgacgttgta gcgctcggcc gccgggatga
     2101 attcgccggg cggcacgagc gtgccgtctt cgtcacgtaa acgcaccatc aactcgaaga
     2161 atggcgtgtc gcccgtgttc gccgagatac cgacgatacg ctgggcatag agttcgaggc
     2221 gcccgtcttc caccgcgcgc gcgatgcggc ctacccactg catctcgcga tggcggttgg
     2281 tgccgtggtc gcgttcgtag atctgcacgc gattgcgtcc actgtccttg gccgtataac
     2341 aggcgatgtc ggcggccgac agcaccgaag cagcactggt ggtgtcgcgc gcgatgcgta
     2401 ccacaccgat gctcgcgccc accgacaggc tgttggcgcc ccagagaaag cgataaccgt
     2461 gaatggcctg gcggatgctg tcggcgacgc gttccgcctg ttccagcgaa catcgctcca
     2521 gcaacaggcc gaactcatcg cctcccaggc gcgcaatggt gtccgaggcg cgcacgcgcg
     2581 tctgcagaag gctggtgatg tcacgcagca ggcgatcgcc ggcgctgtgc ccgcaggtgt
     2641 cgttcacgac cttgaactgg tcgaggtcga catacagcaa cacgtgttcg ccgtcgccgc
     2701 gctgcgcggc gcccaccgcg gtttcgagtc gcaaatcgaa ctcgcgccgg ttgatgagcc
     2761 cggtgagcgc gtcgtggctc gcctggtagg acaacgcgcg tttcaggcgc cgttccttgg
     2821 tgacgtcgcc gaatacgatg accgcgccga tggccacccc ctcgcgattg cggatcggcg
     2881 tcgcggtttc ctggatcgcg agctcctcgc cgcggcgatt caccagcacg atgtgatcgg
     2941 acggtacgga cgtctcgcca ttgatgagca cgcggtcgag cgtataggcc acgggctggc
     3001 gcgtggattc gtcgatgagc tgcagcacgt cgccgatcgg ccggccgcga gcctcgttta
     3061 ccgaccagcc cgtgaggcgt tcggcgaccg ggttcagata gtcgatgcgc gcgccggcgt
     3121 cggtgctgat caccgcgtcg ccgatggact gcagcgtaac cagggcgcga tccttttccg
     3181 cgaaaatggc ctgttccgcg cgcttgcgct cggtgatgtc ggagaccgtg ccttcgaagc
     3241 cgacgatgcg gccgctgcca tcgcgcacgc cgcgtgagtt ctcgagcgcc accacctgcg
     3301 tgccgtcacg ccggcgcagc acgacttcca tcgagcgcac ctcgccatcc gaatcgactc
     3361 gccggacgaa gtcggcgcga tccgtcgcgc tccagtacag catcaccgag ctcggcaatg
     3421 catacatttc ctcggccgag ctgtaaccga gcatcttcac gaaggcactg ttcaccgaga
     3481 ccagccggcc gtcgcgcgtg ctctggtaga cgccttcgat gacgccctcg aacaggccgc
     3541 ggaacttggc ctcgctggcg cgcagggctt cctccgcgag attgcgctcg atggcgagcg
     3601 cggcgagacg cgtggcgtgc gattgcagca cgtgctcgcg ctgatcgggc aggccgggtt
     3661 cggggcggta gacgcccacg gaccctaaca acttgccgct ggcgccccgg atgggcatcg
     3721 accagacggc gctgaacccc gcatcgagca ccacctgccg gcgatcggcc cagtgggcat
     3781 cattggccac gttggacacg aatacgtcgc aggccgaata cacggcggca gcgcacgaac
     3841 cgcggcgcgg ctcgatcggc gttcgctcga gcaccgccgc cagcatcggc ggcaactggc
     3901 gggctatcgt cagcgcgaaa cacgttccat ctgacgccag gaccgatatc gtgcagcggc
     3961 agcgcggata gacggcctgc acgagcgcgg aaatcacgtc gagcactgcc ggcaacccgg
     4021 cgttggacgc gagcaaggcg aagaacgcgc gctcgttttc cattatcaac tcggcgcgct
     4081 tgcgctcgga aatgtcggtg atgctggcgc gcaccagcgg cttcgaaccg ccctgcaggc
     4141 gcgcgaggcg cacttcgcag gggatgtcgc ggccagtgga atcgcgatgg atccactcga
     4201 acacctgcga ttcaccggcg gccgcgagct tcgtgtgctg gcgcggggcc atggcaggct
     4261 gcccgtcggg ctgcatttcg gcactgacgg cgtccaggtg gaccgccagc aattgatctc
     4321 gcgtcatctt gaacagccgc aaggccggct cgttcgcctc gatgaaacgc tgcgtgtccg
     4381 gatcgatgac ggtgatggct tccggcgcgt ggtcgaccag cgagcgataa cgcgcctcgc
     4441 tgtcgcgcaa cgcttcctgc gtgctgtgcc tttcggagat gtcgcgcagg cagatgacga
     4501 acacttcgcc gcgcccggat cttgcgcggc ttacggcaat ctccgcgggg aacggtgagc
     4561 cgtcacggcg gcgcgcggcg acctccgggc tctggcgcat gccgcggaac gtgtcgctgg
     4621 actcagtcaa cctggtgagc cctgccgcaa tcgacccgtc cagtgcgatg ccaggcacga
     4681 gtgcatcgat gttctggcct gacaattctc cggacgcgca ggcaaacacg cgttcggcca
     4741 tcgggttggc gcggtcgatg acccccgccg aatcgacggt gatgaccacg tccttgatgt
     4801 gatcgaggat gacctggagc tgggcggcgc cttgctcggc gatctcgacg ccaagtgatc
     4861 gcgcttcgtc cgacatgagc tgcatggagc gcaccatcgc gcggcgctcg tcgtcgatcg
     4921 agtcgtagta acgactgagc atttccagca gttcatgcgc gctcacccgc ccgcccgtca
     4981 cacgcgcgcg caattcccga agctgcgact gcaaaagtgg atgcaggcgc cgcatcgaag
     5041 aatcgctcat gtccatgcgc ccgccggatg ccgggacggc gcccgtagcg gtcgtggtgg
     5101 aatcagagcc ctcgttgggg ggggcgccct cggcgggttt tgacataacc atgcaagttc
     5161 agaccaaatt gcatgatatg tccggcctgg gagcgcacgc gtgaagcggc tcccggtttt
     5221 gacgtaggcc tagttcttgt ccggctcgcg cgccggttgg gtggctgacg cccctgccgg
     5281 cgccggcgcc gcggcggccg ccacgatgat cgggatcgag aaaatgcgcg tgatgtttcc
     5341 ttcgacaccg acggtatcga cgctggacgt caccatgaac attccttccc tcgtcgggag
     5401 cagcaccagc tgatgcgaca gcggcgtttc gctatcgatg tcggacttgg gaccaaagaa
     5461 atcacccgac gtggtggtca gtccgtcctg gctatcgaaa tgcgcgctca ccgaagtgaa
     5521 gtcgcgatgc ggcacgatgg ccacatcgac agccagcgcc ttgttcaccg tcggcggttc
     5581 gcgcaatgcg aaatgcacgc tgatgacact ggctgacttc ccgccggaaa ccgcggcgac
     5641 catgtctttg gagatgctgg cctccttcgg cggcggatcg ggctttttga cgaccggggc
     5701 gccttgctgc ggctcgggtg gcgcatcgtc acaggcgacg aggacgcagg cgcagatcag
     5761 ggcggcgaac gcagcacggt gacgaatctc gagcatcagg gaccctccgc ggcgaatact
     5821 aacaggcgaa tgctttccta ctcgactggc cggctacctc gacaggatgg ggggatcgtc
     5881 catcgccgag agctgctcga aaaggtcggc tatgtgccgt tcccagaaac gggcgtcgcc
     5941 aaaccagggg aaggctcgcg gaaacgccgg gtcattccag cggctggcga tccacgcggc
     6001 gtgatgcagg atgcgcagcg agcgcaacgc ttccaccagc gtgagctccg cgaaatcgaa
     6061 ctcaccaaac aactcgtagc cttccatgat cgccgcccac gaggactgct ggctggcggc
     6121 atctcccgac aggaacatcc acaggtcctg gatgcgcgga ccggacatgc agtcgtcgag
     6181 gtcgacgaac agcgggccgt tctcgcgcca caggatgttg cccgcatgac agtcgccgtg
     6241 aattcgcagg ccgggaagag gcccgaagtt ttccaggcat tgtcggacgc gcccgatgac
     6301 ctgttcactg acgcgcgcat attgttcctc gagcgcctcg ggtacaaagc cgcttcgctg
     6361 gaccgtggaa cgggcatgta caccgagccg ctcgatggtc agcgccggac gatggcgaaa
     6421 cggcgcgcgg ctgccaatgg cgtgcatgcg cgcgagcgtg cggcctagca aggtcagcgt
     6481 ggccttgtcg tcgagttccg gcgcacgccc tgccaggtac ggaaaagccg cgaatcgcag
     6541 ccgctggtgc tcgaacaggc tctcgccatc gcgttcgatg ggcgcggcga cgggcaattc
     6601 cgccgcggcc atttcgaggg tgaaggcgtg ctcctcgccg atctgcgcat cgctccagcg
     6661 cgcgggacgg taaaacttga gcacccacag ggcaccgtca tcgtcgccca gttgatagac
     6721 gcgattttcg tagctgttga gcgcgaacag gcgcccggac ggttgcagcc cgatgctttc
     6781 cgcagcagcg aaaacggcgc ccgggtcgag tgcatcgaac ggcgcgggtg cagaatgcgt
     6841 cacagccacc gacgcacatt aatgtaaaat ccggtgccct aaaacttttg tccattaaat
     6901 tggtgggatc acttcgatat gccttcgaat tcaacggtcc tggtcactgg cggcgccggg
     6961 tacatcggca gccacacggt cctgcagctg atcgcgcgcg gcgagcgcgt ggtggtgctg
     7021 gacgatctat ccaccggctt tcgccaggcc gtacgcgacg tgccgctggt cgtgggcaac
     7081 gtcggcgatc gacggctggt cgactcggtg ctcgccgagc accgggtgga caccatcgtc
     7141 cacttcgccg cgcataccat cgtccctgaa tccgtcagca acccgctcaa gtactacggc
     7201 aacaacacct gcgcgacccg ctcgctgctg gaagccgcat cgcaggcggg cgtgaagaac
     7261 ttcgtgttct cttccaccgc cgcggtctac ggcattccgg ccaccggcca tgcttcggaa
     7321 gactcgccca ccgcgcccat caacgcctac ggcacctcca agctcatgtc cgagtggatg
     7381 ttgcgcgatc tttgtgccgc gacgccgatg cgtcacgtcg tgctgcgcta cttcaatgtc
     7441 gccgggtccg acccgcaggg ccgcatcggc cagtcgacgc gtgcagccac gctgctggtc
     7501 aaagtcgcct gcgaggctgc ggtcggcaag cgcccttacc tttcggtctt cggcaccgat
     7561 taccccacac ccgacggcac cggcgttcgc gactacatcc acgtcgagga cctggccagc
     7621 gcgcacctga attcgctcga ctatctgcgc gccggcggca attcgctggt ggcaaacgtc
     7681 ggttatggcc atggctacag cgttcgcgaa gtgttgtcgt cggtcgagaa gatagccggg
     7741 ctcaagctcg acgtgcgtga agagccgcgg cgcgccgggg atccaccgag cctggtcgca
     7801 cgttgcgatc gagttcgaga attgctcaag tggcagccgc ggctcgacga catcgacacc
     7861 atcgtgcgca cgtcgttgga gtgggaaaag cgtctgcaac gggaaccgtg gtaatcaggc
     7921 aagctagtta gcctctgatg cgtctgcccc gacccaaatc gctgactggc ctcatgctgc
     7981 tggggctcgc gctcatcgct ggtccgctgc tggtggcggt ggtcgacgcc gccatccaga
     8041 ttcgcagcct ggcgcagtcc agccaggaac tggtgaagga aggcgtgaac agcgcccgcc
     8101 tgtcgcagag cctgttcgcc gacatcacct cgctcgaccg caccgtgcag ctctaccagg
     8161 tgatcggcaa ggtcgagatg ctcaagtcca tccgcgacac cgatcagcgg ctcgcgacca
     8221 cgcgcacgga gctcgcgaag ttgctcaccg aagagcccac gcgccagagc ctcgaagact
     8281 tcgccgccat gcataacgaa atctcgagcg ccgtgggcgt gacgcctccg ggcagcccgg
     8341 ctttcgccac catcctgacg cgcatcgacc gcctcaccga cctcgccaac gccatcgccg
     8401 cgagcggcaa cacgcagatc gacgcgcggc tcgctgaaat gcagcgccag acgtccagca
     8461 cgcaaaagcg cctgttctgg cagtccgcac tgctggcgcc actcacgctc atagcaatcc
     8521 tcgcgctcac gctgtccatc ggccggccgc tgcgagccat cgaccgcgca atcagcgagc
     8581 tcgggcgcgg cacgttttcg cgctccatcg aagtccaggg tccgcgcgac ctcgagcgcc
     8641 tcggcgccca gctggaatgg ctccgcctgc gcctgctcga cctcgcgcag gaacgcaacc
     8701 gctttctgcg ccacatgtcg catgaattga agaccccgct ggccaacatc cgcgaaggtg
     8761 cggaactgct catggatggt gcggtcggac ccctggacaa ctcccaacgc gaggtggtcg
     8821 ccatcctccg tgacaacggc atcaagctgc accggctgat agaaaacctg ctttccttca
     8881 gcgcctggca aaaggagagc gtcgggctcg atctttcgga attccgcctg cggccgctcg
     8941 tcaaacaggt gctcgaaagt cagcaactca cggtagtctc gcaacgcgtc cggctggagg
     9001 ttaaggtgga cgacctgatg ctgcgggccg atcgcggcaa ggtgcgcctc atcatcgaaa
     9061 atctgctgtc gaatgccatc aaatactcgc ccaagggcgg caccgtctat ctacatgccg
     9121 caaaacagga cacccagctc atgatcgacg tcgccgacgc cggctccggc ataccgtcgg
     9181 aggagcgcgc tcgcgtgttc gaggctttct acaccggccg cccacccgcg ggggtccagg
     9241 tcaagggcac cggcatcggg ctgtcggtgg tgctcgagtt cgtgaacgcg cacggcggca
     9301 ccatcgaaat cgtcgacggc gagttcccgg gcgcgcattt ccgcatccgc atgccggtgc
     9361 tgtcggtcac ccatcccgtc cggacacagg cccatgcggc ataagcccct cctggcctgc
     9421 atctcgtgct gcgcgctgtt gaccgccggc tgtggcagcg gcctgccgat caagcgcgat
     9481 actcaggcgc tgcccagcat cgaccgcaat tccaccgaag cgagcctggt cgccgggtac
     9541 cttgaaatct gcatgaaggc cgcgcgcggc acgcccgccg agcaggccga agtgctcgcc
     9601 accacccaca acgactacgc ggcggcgcca acccccagca gggtcctgcg ttatgccatg
     9661 gtgctgtcca cgcccgggca cgcgggctct gatcctgtcg tcgcacagcg acttttgcgt
     9721 gaagtcctag caagccctga gactctgctt cctgcggaac gttctctcgc gtccctgcag
     9781 ttgcagcaag tcgagcgtca gcaatcgctt caagcggatg tgcggcgcct gcagcaggcc
     9841 gccgaaagaa acgcgaacga aaaaatcgcg caactgacgc ggagcctgaa agccgagggc
     9901 gatgaagtaa accgtctcaa gaaggcactt gcggaggccc aggcaaaact ggacgccatc
     9961 gcaaacatcg aaggctcgtc gaataaccgg cggactccga cgcctgaggg gcggaaaccg
    10021 tagaggtttc tgatgcaagc agccatgcaa ttagccacac cgcactccac caagcgcaaa
    10081 gcccgcatcc tggtcgtcga cgacgatccg ggtctgttgc gcctcctgac catccgcctg
    10141 cgtgcggaga actacgaagt ggaagcagtg gaaagcgcgg cgctcgccct cgcggccacc
    10201 agccgctttc gtcccgagct ggtcatcacc gacctgcgaa tggaccagat ggacggcatc
    10261 ggtctgctca aggaactgca gtcccgctac cccggcctca aggtcatcat cctcacggcc
    10321 cacggcacca ttcccgacgc cgtacacgcc acccagctgg gcgcgttcgg cttcctgacc
    10381 aagcccgtcg acaaacaaga gttgctggac caggtgcaga aggcgctgcg catctccggc
    10441 tttgcggaca acgacgaaga ctggcgcgcc gaaatcatca cgcgcagctc gctggtcgaa
    10501 gagaagcttg cccaggcgca catggtcgcc ggcaccgacg cgcgcgtgct cattactggc
    10561 gagagcggca ccggcaagga attgctggcg cgcgccatcc acaatgcgag cccgcgccgc
    10621 gccaagcctt tcgtcgccat caactgcagc gccatggccg agaacctgct cgagtcggag
    10681 ctgttcggcc acatcaaggg ttcgttcacc ggcgcggtgc gggatcacgc cggcctgttc
    10741 caggccgccg atgccggcac actgctgctc gacgagatcg gtgacatgcc catgcgcctg
    10801 caggtcaagc tgctgcgcgt gctccaggaa aacaatatcc gccccatcgg ctccaccgac
    10861 gccattccgg tcaacgtgcg cgtgatctcc gcgacccacc gcgacctgca gcagctcatg
    10921 atgagcggcc agttccgcga agacctgtac taccgcctga acgtcgtgca catcgaaatg
    10981 ccgccgctca accgccgccg cgaagacatc cccctgctcg tctcgcattt cctcgcgcag
    11041 atcagcaacg aaagcggcgt gcgcaagatc tacgcgccgg aagccgtgga gttgttagcc
    11101 acggccgact ggccgggcaa catccgccag ttgtcgaacg tggtccggca gaacgtggcg
    11161 ttgagccaga ctcccatcat cccggtggag ctcgtgcagc agtcgctcgg cggcacgcag
    11221 agcaagctgc cctcgttcga cgaggccaga gacgagttca cgcgctcgta tctctcgcaa
    11281 atcctgcaga tcaccggcgg caacgtgagc caggcggccc gcctcgccaa gcgcaaccgc
    11341 acggacttct acaagctgct gtcgcggcac cagctgaccc cggaagactt caaggggcgt
    11401 tgatcgacaa atcgttgatg gtcgcgtagt ggtcggagcc gacgctctca cggacgtgca
    11461 cgtctgcgac actcactgcg ccgctcacga gacattgatc gatgcggatg cgaagcgccg
    11521 agggcaagcc ggagttccag gtgggtagcc acccgtgacc ggcggcgcaa tcccggacgc
    11581 cgggggccac cgaggtcgac gtctgctcga gcgccataca tcaggtctcc acgtgtcacg
    11641 agttgcggct ctacgagcgg ccagcgactc agggtcaccc agcgccagtg cgaagattga
    11701 ccagcagctc gcaggccctg gaccggcgac cgccgtaggc gagcagggtc gcgaggcaga
    11761 gcaccccggt gccgaacgcc aagggcctcg caacaatgga gtcccggctc aggcctttct
    11821 cacga
//
LOCUS       DASQJS010000376         4201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1490034, whole genome shotgun sequence.
ACCESSION   DASQJS010000376 DASQJS010000000
VERSION     DASQJS010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490034"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VEW08_14865"
     CDS             <1..768
                     /locus_tag="VEW08_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42073.1"
                     /translation="QAIKNEVKRNAPVLFAVQLDWSVTPFDMAKIPPLAIFNAYTLYT
                     TEANREGRTWFGLRLGFFSDAVSAKQVAYYVRSDFKTVSVVPVTTLEKERANDINAAR
                     SGIHRAVNIKEGPAPTTRSITSSNNSGIFKLLEDDLPAMIEQDVDGETSPRFGKQSAA
                     PAATRAAPAPAAAAPPTAPGKASKARKPGARSVRRDAHAQVDEETLDQTLEILGANSL
                     EITNDSAENTGVRHLRVKVDKKGSKFASLIDRLSGKK"
     gene            complement(816..1115)
                     /locus_tag="VEW08_14870"
     CDS             complement(816..1115)
                     /locus_tag="VEW08_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42074.1"
                     /translation="MAHGLTCWKCGASLEALSMPLRRLDECKACHSELHVCRLCVEYD
                     TRVAKHCREPTAEEVRDKTAANFCDHFKPKVGAYIAPTGEADAAKAALEALFGKK"
     gene            complement(1130..3946)
                     /locus_tag="VEW08_14875"
     CDS             complement(1130..3946)
                     /locus_tag="VEW08_14875"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HYJ42075.1"
                     /translation="MAGTPAQVEANPASHTGRALREYEAALVPPLVPGRETGITPAAV
                     RDSTGNYAGVIPVSRPGTAANSIVIHNAREHNLRNIDVTIPREAFTVITGVSGSGKST
                     LAFDIIFNEGQRRYLESLNAYARQFVQPAARPDVDAIYGIPPTVAIEQRTSRGGRKST
                     VATLTEIYHFLRLLYVKLGTQFCPTCDVAIEPQTSRSIAERLLKQHMDSRILLLAPLV
                     VARKGYYTELAKWASARGYKQLRVDGEMMPTNRFPRLNRFKEHTIELPVNQINVQSDS
                     ADLLERNVIRALDMGKGLVHVLDLDSSAKKPKVQVFSIKRSCPSCGTSFSELDPRLFS
                     FNSKHGWCEGCFGTGLALEGFDEEQSGEENAWNDSLEGEAHTCETCDGQRLNQIALHV
                     RFRDLNIADLTHQAIDETVGFVGKLRLNKREAEIARDLMAEIKARMAFLQRVGLGYLP
                     LDRSAPTLSGGEAQRIRLAAQLGSNLQGVCYVLDEPTIGLHPRDNKVLLDALAELAKN
                     RNTLVVVEHDEDTIRRADHVIDLGPGAGVRGGEVVAVGTIEQLKNNPASITGRFLKNP
                     LRHAAEPRRATGTRALRLTLDDVEMHNVRKVDIAIPIGRLVVITGVSGSGKSTVARDV
                     LYANLKRLTAKLPRGAKQPELTGVAAVHGVEHINRVLEVDQTPIGKTPRSCPATYIGF
                     WDDIRRIFANTTEAKIAGYSTSRFSFNTGEGRCPACEGQGMQTIEMNFLPDVKVLCDS
                     CRGRRFNSETMSILWRGKSVGDLLEMNIDDAVEFFAAHASIHHALRLLQDVGLGYLTL
                     GQPSPTLSGGEAQRIKLVTELAKVRGPERRAPRQGPQHTLYVLDEPTVGLHMADVEKL
                     IHVLHRLVDAGNSVVVIEHNLDVMAEADWIIDMGPEAGAGGGRIVAAGPPSVIMKVAG
                     SHTGTVLKEFLKNGARHRLPENGA"
     gene            complement(3923..4138)
                     /locus_tag="VEW08_14880"
                     /pseudo
     CDS             complement(3923..4138)
                     /locus_tag="VEW08_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          725 a         1319 c         1370 g          787 t
ORIGIN      
        1 caggccatca agaacgaagt gaagcgcaat gcgccggttc tgttcgccgt gcagctcgac
       61 tggtcggtta cacccttcga catggcgaag attccgcctc tcgccatctt caacgcctac
      121 acgctctaca ccaccgaagc caatcgtgaa ggccgcacct ggttcggcct gcgactgggc
      181 ttcttcagcg acgcggtttc cgcgaagcag gtggcgtact acgtgcgctc cgatttcaag
      241 accgtgtcgg tcgtgcccgt caccaccctc gaaaaagagc gcgccaacga catcaacgcg
      301 gcgcgcagcg gcattcaccg ggctgtgaac atcaaggaag gaccggcccc cacgacccgg
      361 tcaataacct cgtcgaacaa cagtggcatc ttcaagctgc tcgaagacga cctgcccgcg
      421 atgatcgagc aggacgtgga cggcgagact tcaccgcgct tcggcaaaca gtctgcggcg
      481 cccgccgcca ccagggccgc tcccgcaccc gcggccgctg cgcctccgac tgctcccggc
      541 aaggcatcca aagcgcgcaa gcccggtgct cgcagcgtgc gccgcgatgc gcatgcgcag
      601 gtggacgaag agacgctcga tcagacgctg gagattctcg gcgcgaactc gctcgagatc
      661 accaacgaca gcgcggaaaa caccggcgtg cggcatctgc gcgtcaaggt ggacaagaaa
      721 ggctcgaagt tcgcctcgct gatcgaccgg ttgtcgggca agaaatagcc gtcctcgcgg
      781 gctgatctcc gccgcgtccc cctgggggcg cctcactatt tctttccaaa gagcgcttcc
      841 agcgctgcct tcgcagcgtc ggcttctcct gtcggcgcga tgtaggcgcc caccttcggc
      901 ttgaagtgat cgcagaagtt ggcggcggtc ttgtcgcgca cttcttcggc cgtgggctcg
      961 cggcaatgct tggcgacacg cgtgtcgtac tccacgcaca ggcgacacac gtgcagctcc
     1021 gaatgacagg ccttgcactc atcgagccgc cgcaacggca tcgacaaagc ttcgagtgaa
     1081 gcgccgcatt tccagcaggt gaggccgtgt gccataccca ataatggtgt caggcaccgt
     1141 tttctggcaa acggtgcctg gcaccattct tcaagaactc cttgagtacc gtgccggtgt
     1201 gtgacccggc gaccttcatg atgacgctgg gcgggcccgc cgccacgatc ctgccgccgc
     1261 cggcgcccgc ttcgggcccc atgtcgataa tccagtccgc ttcggccatg acgtcgagat
     1321 tgtgttcgat cacgacgacg gaattcccgg cgtccaccag gcgatgcagc acgtggatga
     1381 gcttctcgac gtcggccatg tgcagtccga cggtgggttc gtcgagcacg tacaacgtgt
     1441 gctgcggacc ttgcctcggt gcgcggcgtt cgggaccgcg caccttggca agttcggtga
     1501 cgagcttgat gcgctgggct tcaccacccg acagcgtggg gctgggttga ccgagtgtga
     1561 ggtagccgag gcccacgtcc tgcagcaggc gcaatgcgtg atggatcgag gcgtgcgccg
     1621 cgaaaaattc cacggcgtcg tcgatgttca tctctaacag gtcaccgacc gacttgccgc
     1681 gccacaggat ggacatggtc tcggagttga agcgccggcc acggcacgaa tcgcacagca
     1741 ccttcacgtc gggcaggaag ttcatctcga tggtctgcat gccctggcct tcgcaggccg
     1801 ggcaacgtcc ctcaccggta ttgaacgaga agcggctggt gctgtagccc gcaatcttcg
     1861 cctcggtggt attggcgaat atgcggcgaa tgtcatccca gaagccgatg tacgtggccg
     1921 ggcaggagcg cggcgtcttg ccgatgggcg tctggtccac ttcgagtacg cggttgatgt
     1981 gttccacgcc gtgcaccgcg gcgacacccg tgagctcagg ttgtttggcc ccgcgcggca
     2041 gcttggcagt gagccgcttg agattcgcgt atagcacatc gcgcgccacc gtggattttc
     2101 cggaacccga aacccccgtg atgacgacca gcctgccgat tggtatggcg atgtccacct
     2161 tgcgaacgtt gtgcatttcg acatcgtcga gcgtgagcct gagcgcgcgc gtcccggtgg
     2221 cgcggcgcgg ctcggccgca tgccgcagtg gattcttgag aaatcgcccg gtgatggacg
     2281 cgggattgtt cttgagctgt tcgatggtgc cgacggccac gacctcgccg ccgcgcacgc
     2341 ccgcgccggg cccgagatcg atgacatggt cggcgcggcg aatcgtgtct tcgtcgtgtt
     2401 cgacgacgac cagcgtgttg cggttcttcg ccagctcggc cagcgcatcg aggaggacct
     2461 tgttgtcgcg cgggtgcagg ccgatggtgg gttcgtcgag cacgtaacac acgccctgca
     2521 ggttggaccc gagctgcgcc gcgagccgga tgcgctgcgc ctcgccgccc gagagggtgg
     2581 gcgcggagcg atcgagcggt agatagccga ggccgacgcg ctgcaggaag gccattcgcg
     2641 ccttgatttc cgccatgaga tcgcgcgcga tctcggcttc acgtttgttg aggcgcagct
     2701 tgcccacgaa gcccacggtt tcgtcgatgg cctggtgcgt gagatcggcg atgttcaggt
     2761 cgcggaagcg gacgtgcagc gcaatctggt tgagccgctg cccgtcacag gtttcacagg
     2821 tgtgcgcctc gccttcgagc gaatcattcc atgcgttctc ttcgccgctt tgttcctcgt
     2881 cgaatccttc gagcgccagg cccgtgccga agcagccttc gcaccagcca tgcttggagt
     2941 tgaacgagaa cagccgcgga tcgagctcgg agaagctcgt gccgcaggaa gggcaggagc
     3001 gcttgatact gaaaacctgc accttgggtt tcttcgcgct ggagtcgagg tcgagcacgt
     3061 gcaccagacc cttgcccatg tcgagcgcgc ggatcacgtt gcgctccagc aggtcggcgc
     3121 tgtcgctctg cacgttgatc tggttcaccg gcagctcgat ggtgtgttcc ttgaagcgat
     3181 tcagccgcgg gaagcggttg gtcggcatca tctcgccatc gacgcgcagt tgtttgtagc
     3241 cgcgcgcgct ggcccatttc gcaagctcgg tgtagtagcc cttgcgcgcc acgaccagcg
     3301 gggccagcag caggatgcgc gagtccatgt gctgcttgag gagacgctcg gcgatggacc
     3361 tcgaagtctg cggctcgatg gcgacgtcac aggtggggca gaactgcgta cccagcttca
     3421 cgtagagcag gcgcaggaag tgatagatct cggtgagcgt ggccaccgta ctcttgcgtc
     3481 cgccgcggct ggtgcgctgt tcgatggcca ccgtgggcgg aatgccatag atcgcatcca
     3541 cgtccgggcg cgccgcgggc tgcacgaact ggcgcgcata ggcgttcagg gattcgagat
     3601 agcggcgttg gccttcgttg aagatgatgt cgaacgccag cgtggatttg cccgagcccg
     3661 acacaccggt gatgacagtg aaggcttcgc gagggatcgt gacgtcgatg ttgcgcaggt
     3721 tatgttcgcg cgcgttgtgg atgacgatgg agttggcggc ggtgccgggc cgggaaaccg
     3781 ggataactcc ggcgtagttc ccagtgctgt ctcgaacggc ggccggagtt atcccggttt
     3841 cccggcccgg caccaatggc ggcaccaatg ccgcctcata ctcacgcaat gcgcggccag
     3901 tgtgcgacgc cggattcgct tcgacctgcg ccggagttcc ggccactacg atttcgccac
     3961 ccgcgtcacc gccctcgggg ccgagatcga tcagccagtc cgaagagcgg atcacgtcga
     4021 gattgtgttc gatcaccagc agcgagtggc cgatcttcag cagcttgcgg aaggcgcgca
     4081 gtagtttggc gacgtcgtcg aaatgcaggc cggtggtggg ttcatcgaac aggaacagct
     4141 tgccgcgaat cgacggcgtg tcgtcggcgg cggcctcggc caggtggccg gcaagcttca
     4201 g
//
LOCUS       DASQJS010000377         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3066362, whole genome shotgun sequence.
ACCESSION   DASQJS010000377 DASQJS010000000
VERSION     DASQJS010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3066362"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VEW08_14885"
     CDS             complement(<1..212)
                     /locus_tag="VEW08_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42076.1"
                     /translation="MIRALMLVMTAVATASSTALTPSEFTLSEACPPGFEKTGAGACE
                     LRTLYQFYPVKRGLGGTRTALPPHRDG"
     gene            complement(209..847)
                     /locus_tag="VEW08_14890"
     CDS             complement(209..847)
                     /locus_tag="VEW08_14890"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYJ42077.1"
                     /translation="MQPSHSRSIASWIGALLLICSAASFAGDGASLFAPCVACHGKSA
                     EGNLALNAPAIAGQDAAYLDRQLHNYRRNRRGTHKSDALGAQMRAVAATLADDSAVAM
                     IARYVASLPKTVSSVPARGNLHNGNNLYQGKCGACHGNTAEGNPALKSPRLAGLDAAY
                     IKRQFAHFRDGVRGTDPQDIPGRQMAMMARTMPNERDLDDVIAFIHQQGRVK"
     gene            complement(1158..1547)
                     /locus_tag="VEW08_14895"
     CDS             complement(1158..1547)
                     /locus_tag="VEW08_14895"
                     /inference="COORDINATES: protein motif:HMM:NF020434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HYJ42078.1"
                     /translation="MSRPAHSAYRSLPLVYSCSGCSSAAQLANHVAVRLDRQGLAEMS
                     CIAGVGGDVDSLVRTAAAGRRIIALDGCPLQCVKNCLRRHGIAPTLQYTLSELGIRKR
                     YHADFDVSDADRVIARVRDDLASPQPA"
     gene            complement(1569..1910)
                     /locus_tag="VEW08_14900"
     CDS             complement(1569..1910)
                     /locus_tag="VEW08_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42079.1"
                     /translation="MKIRSPAAVFSLIALVATPFAAHANGSTRATDACIRAFVDSYLL
                     KDRPMQVQKLSSVQGAQSFYGKRYTIALNARLASNGTELVTAQCVASTNGEVISLDLA
                     PATTGPTASAN"
     gene            complement(2069..>2296)
                     /locus_tag="VEW08_14905"
     CDS             complement(2069..>2296)
                     /locus_tag="VEW08_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ42080.1"
                     /translation="SIAHVLGKVTVAEGVETIEQVRTLRQLGCDFAQGFFFAKPVPAK
                     DLPAVVARVPAWRQIVSDSVADQERPRVAAR"
BASE COUNT          413 a          684 c          751 g          448 t
ORIGIN      
        1 ccgtcgcggt gcggtggcag cgcggtgcgc gtgccgccga ggccgcgttt cacagggtag
       61 aactggtaga gcgtgcgaag ttcgcacgcg ccggcgccgg tcttttcgaa gccgggtggg
      121 caggcctcgc tgagagtgaa ctcagaaggt gtcagcgcgg ttgagctggc cgttgccacg
      181 gccgtcatca ccaacatcag cgccctgatc acttgacgcg accctgctgg tgaatgaacg
      241 cgatgacatc atcgaggtcg cgctcgttcg gcatagtcct ggccatcatg gccatctgcc
      301 ggccggggat atcctgcgga tccgtgccgc gcaccccgtc gcgaaagtgc gcaaactgcc
      361 gcttgatata ggcggcgtcg aggcccgcga gtcgtggtga cttcagcgcc ggattgccct
      421 cggccgtgtt gccatgacag gcgccacact tgccctggta gaggttgttg ccgttgtgca
      481 ggttgccgcg cgccggaact gagctgacag tcttgggaag gctcgcgacg tatctggcga
      541 tcatcgcgac cgctgagtcg tcggcgagtg tggccgcaac ggcccgcatc tgcgcgccga
      601 gcgcgtcgga cttgtgtgtg ccgcgacggt tacgtcgata gttgtgcagc tggcgatcga
      661 ggtatgccgc atcctgcccg gcgatggcag gcgcgttcaa cgccaggttg ccctcagcgc
      721 tcttgccatg gcacgcgacg catggtgcga acagactcgc cccatcgcct gcgaacgatg
      781 cggcgctgca tattagaagg agtgcgccga tccatgacgc gatcgatcgg gaatggcttg
      841 gttgcatcag agagggtggc gctgcggaat gtcgattgtc gccgggagcg gtgatacctg
      901 acctaacaat gaatcgcaat cagggcaccg ctgatgccag atattcacac tcattttgac
      961 gcagtgcgta gagtctggac gagaagtcaa aaatttacgc accgctcatg cgcggttcgc
     1021 cgtgcctgac acgggcgatt caaatcgagc ggcgcgattc tggcgaggat ctgcgagaag
     1081 cgaatgtccg accccgccaa cttcttgtta ttttctgcca atactcactg gccgcctcct
     1141 gcgccagtca cgcgcgctca ggccggttgc ggcgaggcca ggtcgtcacg cacgcgcgcg
     1201 atcacgcggt cggcatcgct cacatcgaag tccgcgtggt agcgcttgcg aatgccaagc
     1261 tccgacaacg tgtattgcag cgttggcgca atgccatgcc gccgcaggca gttcttgaca
     1321 cactgcagtg ggcatccatc cagcgcaatg attcgtctgc cagccgcggc tgtacggacg
     1381 agcgaatcga cgtcaccacc gactcccgcg atgcacgaca tctcggcaag tccctggcga
     1441 tcgagccgca ccgctacgtg gttggcgagc tgcgcggcgc tcgagcagcc ggaacacgaa
     1501 tagaccagag gcaggcttcg gtacgcgctg tgcgccgggc gtgacatgat cgtgcagggg
     1561 caatgagatc agtttgccga cgcagttggc ccggtagtgg cgggtgcaag gtcgagtgaa
     1621 atcacctcgc cgttggtgct ggcgacacat tgcgccgtca caagctctgt gccattgctt
     1681 gccagccgtg cgttcagcgc aatcgtgtac cgtttcccgt agaaactctg cgcgccctgc
     1741 acggaagaca atttttgtac ctgcatcggg cggtctttca gcaggtatga atcgacgaac
     1801 gcccgaatgc aggcgtcggt tgctcttgtc gacccgtttg cgtgggcagc gaaaggcgtc
     1861 gcgaccaggg ctatgagact gaagactgcg gccggactgc gaattttcat cggatgttcc
     1921 tcgattcgga cctttcttga tgtatgcatt attgggcggt ggttgccgcg acgccctgat
     1981 ccagatcagt tgccgcatcg aagatatgtg aaacagcatg aggcctgcga ttcggtgatg
     2041 cggggcgcgc atcggggaaa cggcggtgtc agcgcgccgc aaccctcggt cgctcttggt
     2101 cagcgacgga gtcgctcacg atttgccgcc aggcggggac gcgagcgacc accgcaggga
     2161 gatctttcgc gggcaccggc tttgcgaaga aaaatccctg cgcgaagtcg cagccgagtt
     2221 ggcgcaaggt gcggacctgt tcgatcgttt cgacgccttc cgcgaccgtg accttgccga
     2281 gcacgtgcgc gatgga
//
LOCUS       DASQJS010000378         4147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4322153, whole genome shotgun sequence.
ACCESSION   DASQJS010000378 DASQJS010000000
VERSION     DASQJS010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4322153"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VEW08_14910"
     CDS             <1..398
                     /locus_tag="VEW08_14910"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HYJ42081.1"
                     /translation="WLLLIAAGVTPKDHLPYWVVACCFLAIGLGTLFGGWRIVKTMGQ
                     RITKLKPVGGFAAETGGALSVGLATFLGVPVSTTHTITGAIVGVGAAQRSSAVRWGVA
                     GNIVIAWIVTIPASAGIAAIAWWLGGLLF"
     gene            complement(395..898)
                     /locus_tag="VEW08_14915"
     CDS             complement(395..898)
                     /locus_tag="VEW08_14915"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HYJ42082.1"
                     /translation="MFDRRQKINLACALICAGMLGYAIYAEKYLGFEPCPLCMFQRVC
                     IGALGIVFLIAAVHRVRYQGAIVYGGFILVAAAAATWVAGRHVWIQHQPPGTVPACGA
                     PLDNLLQMFPLLEVIRKVMTGGGECGNIDWTLLGISMPGWVLIAAVSLGLVGILNNAV
                     YPVRARR"
     gene            complement(987..1712)
                     /gene="phoU"
                     /locus_tag="VEW08_14920"
     CDS             complement(987..1712)
                     /gene="phoU"
                     /locus_tag="VEW08_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HYJ42083.1"
                     /translation="METADLSHHISRRFNEDLERVRNKVLTMGGMVEQQLANAVTALI
                     EGDSNLGVSVGRDDFKVNGMEVSIDEECSRILATRAPAAGDLRLIVAIIKTITDLERI
                     GDEGEKIGYIASRLATMERPADKYREVKHLGRIVSEMVHQALDAFARMDSEAALRVAR
                     QDRVVDEEYESIQRQCITFMMEDPRSIRRALDVMWIVRALERIGDHAKNICEYVIYMV
                     HGKDVRHTKLEDIERDLRDRGAG"
     gene            complement(1761..3074)
                     /gene="phoR"
                     /locus_tag="VEW08_14925"
     CDS             complement(1761..3074)
                     /gene="phoR"
                     /locus_tag="VEW08_14925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HYJ42084.1"
                     /translation="MNSGATAFVYVVLRILAALAAGAMLGWLAGSVWLGISGALGLYL
                     AWNLWQLRALDFWLQHRSIADPPDAMGLWGDVVAQVVRLHRRKRFHKEKLTRLFRELR
                     RSTAAMPDGVVMLDPQGEIVWFNRKAGELLDLSRRADLGLRIDNLVRHPDFVRYLHGG
                     QFSMPVIVRLDTPTERFIAFQVISYGEDQRLLMLRDVTREVRMEQMRKDFVANASHEL
                     RSPLTVVAGYLENFSADPGLGELAAPVAEMRRQTDRMTRIIEDLLELSRFEANDAPIK
                     GQPIDVSAMASMLRKDVLARPVHPRQVEVVIESAAQLIGEEAMVQSAFSNLVDNAAKY
                     TPPDGSMQIRWWTDEEGGHFSVRDTGPGISSEHIPRLTERFYRVDPGRSRETGGSGLG
                     LAIVKHALHRHGGRLEIESVEGRGSTFTCHFPHDRVLERRVAASA"
     gene            complement(3079..3774)
                     /gene="phoB"
                     /locus_tag="VEW08_14930"
     CDS             complement(3079..3774)
                     /gene="phoB"
                     /locus_tag="VEW08_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HYJ42085.1"
                     /translation="MSAKQILVVEDERPIREMIAFGLRRAGFEVREAADARTGRAEVA
                     NKLPDLLLVDWMLPDTSGLEFTRALKRDRETRELPVIMLTARAEEGDKVAGLEGGADD
                     YITKPFSPRELLARINAVLRRTTPVEGTERIDVEGLVLDQSSQRVSSGERTVALGPTE
                     YRMLAFFMTHPERVYTREQLLDRVWGGNVYVEERTIDVHIRRLRKALEEYGYDRLIQT
                     VRGSGYRFSARPL"
     gene            complement(3847..>4147)
                     /locus_tag="VEW08_14935"
     CDS             complement(3847..>4147)
                     /locus_tag="VEW08_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42086.1"
                     /translation="TFEVDGVLTSGAEHVSAAAAVLLPPDTSGAWAAAGRGLLAWPAL
                     AESCRVAGARLHADLLPRASEVLALARLAVAAGQILDPADALPVYVRDRVTQSSV"
BASE COUNT          718 a         1343 c         1351 g          735 t
ORIGIN      
        1 tctggctgct gctgattgcc gcgggagtga cccccaagga ccacctgcct tactgggttg
       61 tggcttgctg cttcctcgcg attggccttg ggacgctgtt tggcggctgg cgcatcgtca
      121 agaccatggg ccagcgcatc accaagctga agccggtggg tggattcgcg gctgaaaccg
      181 gcggcgcatt gtcggtgggc ctggccacgt tcctcggtgt accggtgtcg accacccaca
      241 cgatcaccgg cgccatcgtc ggtgtcggcg ccgcgcagcg ttcatctgcg gtgcgctggg
      301 gtgtggccgg caacatcgtc atcgcctgga tcgtgaccat ccctgcctcg gccggcatcg
      361 ccgccattgc gtggtggtta ggcggcctgc tcttctagcg ccgcgcgcgc accggataga
      421 ccgcgttgtt gaggatgccg acgaggccca gcgacacggc cgcaatcaga acccaaccgg
      481 gcatggaaat gccgagcagc gtccagtcga tgttgccgca ctccccgccc ccggtcatga
      541 ccttgcggat cacctcgagc agcggaaaca tctgcagcag gttgtcgagc ggcgcgccgc
      601 aggccggcac cgtgcccggc ggctgatgct ggatccatac atgacgcccg gccacccagg
      661 tcgccgcggc cgcagccacg aggatgaagc cgccgtagac gatggcgccc tggtaacgca
      721 cgcgatgcac ggcagcgatg agaaagacga tgcctaacgc gccgatgcac acgcgctgga
      781 acatgcacag cgggcagggt tcgaagccaa gatatttctc ggcgtatatc gcgtagccca
      841 gcattcccgc gcagatgagg gcgcaggcga gattgatctt ttgccgtcgg tcgaacatgg
      901 gcttgccagg cgctcgcacg ccacttcgga aaacgccgtc gaagcgacgg cgtccgagaa
      961 tagcccagag atagaaacgg gtgctactaa cctgcgccgc gatcgcgcag atcgcgctcg
     1021 atgtcctcga gtttcgtatg gcgcacgtct ttgccgtgga ccatgtagat cacgtattca
     1081 cagatgttct tggcgtgatc accgatgcgt tccagcgccc gcacgatcca catgacatcg
     1141 agcgcgcggc ggatgctgcg cggatcttcc atcatgaagg tgatgcactg gcgctggatg
     1201 gattcgtatt cctcgtccac cacgcgatcc tgccgcgcca cgcgcagcgc ggcttcgctg
     1261 tccatgcgcg cgaaggcgtc gagcgcctgg tgcaccattt ccgagacgat gcggccgagg
     1321 tgtttgacct cgcgatactt gtccgcgggc cgctccatgg tagcgagcct cgatgcgatg
     1381 tagccgatct tctcgccctc atcgccgatg cgctcgaggt cggtgatggt cttgatgatg
     1441 gcgacgatga gacgcaggtc gccggcggcc ggggcgcgcg tcgcgaggat gcgcgagcac
     1501 tcctcgtcga tcgagacctc catgccgttg accttgaagt cgtcacggcc cacggagacg
     1561 ccaaggttgc tgtcgccctc gatgagggcg gtcacggcat tggcgagctg ctgctcgacc
     1621 atgccgccca tggtcagcac cttgttacgc acgcgctcca ggtcttcgtt gaagcggcgc
     1681 gagatgtggt gcgaaagatc ggcggtttcc atgggttcct caggcgcagt ttgttagtgg
     1741 gcgatgacgt gctgatgaca tcaggcactc gctgcgacgc gccgctcgag cacgcgatcg
     1801 tgcgggaagt ggcacgtgaa ggtgctgcca cgcccttcga cgctttcgat ttcgagccgg
     1861 ccaccgtggc gatgcaatgc gtgttttacg atggccagcc ccagccccga gccgccggtc
     1921 tcgcgtgagc ggcccgggtc gacacggtaa aagcgctcgg tgagacgcgg tatgtgctcg
     1981 ctggaaatgc ccgggccggt gtcgcgcacc gagaaatgcc cgccctcctc gtcggtccac
     2041 cagcggatct gcatgctgcc atcaggtggc gtgtatttgg cggcgttatc cacgagattg
     2101 gaaaacgccg actgcaccat cgcctcttct ccgatgagtt gcgccgccga ctcgatgacg
     2161 acttcgacct gccgcggatg caccggacga gcgagcacat ccttgcgaag catcgacgcc
     2221 atcgccgaaa catcgatggg ctgacccttg atcggcgcat cgttggcctc gaagcgcgac
     2281 agctcgagca ggtcctcgat gatgcgtgtc atgcgatccg tctggcggcg catctcggcg
     2341 accggcgcag ccagctcgcc gagacccggg tcggcgctga aattctcgag atatccggca
     2401 accacggtga gcggcgagcg cagttcgtgc gaagcattgg ccacgaaatc cttgcgcatc
     2461 tgctccatac ggacttcgcg cgtgacgtcg cgcagcatga gcagtcgctg gtcctcgccg
     2521 tacgagatga cctggaacgc aatgaagcgt tccgtcggcg tgtcgaggcg cacgatgacc
     2581 ggcatggaga attgaccacc gtgcaggtaa cgcacgaagt cgggatggcg aaccaggttg
     2641 tcgatgcgca ggcccaggtc cgctcgccgc gacagatcga gcaactcgcc ggccttgcga
     2701 ttgaaccaca cgatttcgcc ctgcgggtcg agcatgacca caccatcggg catggcggcg
     2761 gtggaacgcc gcaattcgcg gaacagccgt gtgagctttt ccttgtgaaa gcgcttgcga
     2821 cgatgcaggc gcactacctg tgccaccaca tctccccaca ggcccatcgc atccggtggg
     2881 tcggcgatgc tgcggtgctg aagccagaaa tccagtgcgc gcagctgcca gaggttccaa
     2941 gccaggtaca gcccaagtgc gccgctgatg ccgagccaga ccgaaccggc gagccacccg
     3001 agcatcgcac cggctgcgag cgccgcgaga atccgcagca cgacgtagac gaaggcggtg
     3061 gcgcccgaat tcatcgctct agagtggacg cgccgagaac cggtaccccg aaccgcgcac
     3121 ggtctggatc agccggtcgt agccgtattc ttcgagcgcc ttgcgcaggc gacggatgtg
     3181 gacgtcgatg gtgcgctcct ccacatagac gttgccgccc cagacgcgat cgagcagctg
     3241 ctcgcgcgtg tacacgcgct ccgggtgcgt catgaagaag gccagcatgc gatattcggt
     3301 ggggcccagc gccacggtgc gttcgcccga gctcacgcgc tggctgctct ggtcgagtac
     3361 cagaccctcg acgtcgatgc gctcggtgcc ttcgaccgga gtcgtgcgcc tcagcacggc
     3421 gttgatacgc gccagcagtt cgcgcggcga aaagggtttg gtgatgtagt cgtcggcgcc
     3481 gccttcgagc cccgcaacct tgtccccctc ttcggcgcgc gcggtgagca tgatgaccgg
     3541 cagctcgcgc gtttcgcggt cgcgcttgag cgcgcgcgtg aactcgaggc cgctggtatc
     3601 gggaagcatc cagtcgacca gcagcaggtc gggcagcttg ttggcgactt cggcgcgacc
     3661 ggtgcgggca tcggccgctt cgcggacttc gaacccggca cgacgcaatc cgaacgcgat
     3721 catctcgcgt atggggcgtt cgtcctcgac taccagaatc tgtttggctg acatggttgc
     3781 tgacacggca tcacaggtga cgtttttgtg gcgtgcgcat tacaacggca gtctattgca
     3841 gtttgattac accgaggatt gcgttacacg atccctgaca taaacgggca acgcgtcggc
     3901 tggatcgagg atttggccgg ccgccaccgc gagccgcgcg agagccagca cctcgctggc
     3961 acgcggcagc aggtcggcgt gcaatctggc gcccgccacc cggcagctct cggccaacgc
     4021 gggccaggcc aggaggccgc gtccggcggc agcccaggcc ccgcttgtgt ccggcggcag
     4081 gagcaccgcc gccgcggcgc tcacgtgttc cgcgcccgac gtgagcacac catcgacctc
     4141 gaacgtc
//
LOCUS       DASQJS010000379         6742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4012343, whole genome shotgun sequence.
ACCESSION   DASQJS010000379 DASQJS010000000
VERSION     DASQJS010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4012343"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VEW08_14940"
     CDS             <1..427
                     /locus_tag="VEW08_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42087.1"
                     /translation="GLDVQAREALWKVVRELRHEGCSIVLTTHYLEEAEALADRVAVM
                     ARGRLIAGGSVNEIRAHVTRKQVSCVTRLTADTVSAWPEVVQVELERDRLKITTRNAE
                     ALLVQLFREDPQLSDIEVKRAGLAEAFTELTNDSKKEAA"
     gene            424..1881
                     /locus_tag="VEW08_14945"
     CDS             424..1881
                     /locus_tag="VEW08_14945"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HYJ42088.1"
                     /translation="MNAVSNMSGSRLWAAYLGEMRFEFIKSLRTPAFAVPTLLFPILF
                     YVMFGIFFGSMRGNSGQAAYLFATYGVFGAMGPGLFGFGVSLAIEREQGLLTLKQALP
                     QPPGAYLLARAAMAMLFVGIIALMLTSMAVFVGHVPLTFSQGIRLFAIDVFGALPFCA
                     IGMWVGSMVSGAASPAIVNLIFLPMAFLSGLWVPLQFMPKVLIDFAPIWPSYHLAQMA
                     LSTVGEKSSGTTMGHVAVLAGVTLVFFLLAMRRMHGSGFRLLGAQPKRTLAIVAGVCA
                     AVIALSLSGVFGGRETDNASPGTASVATGTPAAEAAAGIAAPQVVVLSNFDAGSAATA
                     YGVGWSAAGDDMRGGNSHASQRVVDDGAGGSKGALEVTGAVGDQIQYPFAGTMFFPEG
                     PPMQGLMDYSGKKTLSFQARGDGRRYMLMVISGLAVEGIPLMVDFETGPEWHEVRLEI
                     AKLGAADWKRVRAIGVGTMGPAGAFRFQIDDVRVE"
     gene            1904..3046
                     /locus_tag="VEW08_14950"
     CDS             1904..3046
                     /locus_tag="VEW08_14950"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYJ42089.1"
                     /translation="MRLKQLLQNIREPPPTSMVGRMIGVDGRVMRNMPFVILFNLAWL
                     FVYPAIAGAPFVRVILPTIISLPLFVYLHLCSYYYGGSNEVRLRYIAGIFLLGLALTY
                     FNLAAVGYLIFGYFSVAFAVPTRIAWKVIFGVCAAYVIEIILLGHNTQTVLSCTLPAA
                     LLGVSALYAAYTTHQQMALRRSNEEILRLATMAERERIGRDLHDLLGHTLSVVALKSE
                     LARKLIDRDLEAARKEISEVERVARDALSQVRNAVSGIRSTALSAELVAATALLEAQG
                     VKVKCETENVKLPHDRETALALSLREAATNIRRHSGASGVTIRVRKESSAVVVEVADD
                     GRGGRIVPGNGLNGMRERLDTVGGTLSLTANENGGTLLRASVPVAA"
     gene            3057..3659
                     /locus_tag="VEW08_14955"
     CDS             3057..3659
                     /locus_tag="VEW08_14955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ42090.1"
                     /translation="MIRILLAEDQAMVRGALGALLRMERDIEVLGDAADGMQALSECR
                     RLKPDIVVTDIEMPGMTGIELAQKIQADGLSCKVVIVTTFARQGYLRRALDAGVKGYL
                     LKDSPAEKLAEALRNVHRGGKAIDPALAVEAWNESDPLNDRERQTLRLSGEGLSAPQI
                     AEQLGLSAGTVRNYLSEAIGKLGANNRIDAYRLARQRGWL"
     gene            complement(3768..4490)
                     /locus_tag="VEW08_14960"
     CDS             complement(3768..4490)
                     /locus_tag="VEW08_14960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ42091.1"
                     /translation="MTEKRVALITGAGSGIGKATAIAFLRDGFRVVLAGRRADKLEAV
                     AREAGDAAQSLCVVTDVTKPDEVRALFDATHEKFGRLDVLFNNAGLNAPFLPLEDMPY
                     EKWREVIDTNLTGAFLCTQQAFRLMKDQTPRGGRIINNGSISAHAPRPNSAPYTASKH
                     AITGLTKATSLDGRKYDIACGQIDIGNALTQLGSRLPDPEPMMDVSHVANAVLQMAKL
                     PPEANVQFMTILATKMPYIGRG"
     gene            complement(4506..4796)
                     /locus_tag="VEW08_14965"
     CDS             complement(4506..4796)
                     /locus_tag="VEW08_14965"
                     /inference="COORDINATES: protein motif:HMM:NF025614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HYJ42092.1"
                     /translation="MNSPEKPLTDAEVDALYGLEPVIAGDAATFDSAASSEFTRVSCP
                     YCGEEFETSVDASAGLCSYVEDCQVCCQPIEMELRVDENGAFAELLLRRGDA"
     gene            4795..5364
                     /locus_tag="VEW08_14970"
     CDS             4795..5364
                     /locus_tag="VEW08_14970"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HYJ42093.1"
                     /translation="MFTRGSMMARLALLVGISLVTMGNFAFCSFNSGPGGGTGLPGGP
                     DGPGGNDSFRSTLVLRNVSGVETSSFVFGEAIRFDFAVENRTARQLRLSFPDAQTTDY
                     LVVNQGTTQIRWMWSEGQSFAQVATELVFEPYSTRSFSVTWNGNLSDGTNLPPGDYQA
                     RGLMVFDGYETNPLAPSELAAPLVPFTVR"
     gene            5441..5989
                     /locus_tag="VEW08_14975"
     CDS             5441..5989
                     /locus_tag="VEW08_14975"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HYJ42094.1"
                     /translation="MRFRAFSAALAAATALALPVAGFGVEPELNIPNFSHLKHKATES
                     VDVTLDGFLMRIAQKFAASHAAEEAHDEAISILQDIKSVRVRNFTFDSDNAYSMADID
                     SVRKQLKAPGWSALVQVHRRDNEDVDVYVCLEDDKVKGLAVIASEPREFTIVNIVGSI
                     DLDKISQLEGEFGIPRMSEQVE"
     gene            6022..6588
                     /locus_tag="VEW08_14980"
     CDS             6022..6588
                     /locus_tag="VEW08_14980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ42095.1"
                     /translation="MSLSAAQPMPDSLLAQARAGDRDAFATLVRAHQGSVFSIGLRML
                     SRRDAAEDLAQDVFLQLYRKLDSIESLEHLGFWLRRVAANLAIDWLRRLPYSATRPLE
                     EGAEVAAQEKDQDLLMSRELMRLLGELAPHPRAVMLLRYQEDRDVAEIAAMLDMPVNT
                     VKSHIKRSLTALRGQMIGAKLVDCEELP"
BASE COUNT         1183 a         2182 c         2118 g         1259 t
ORIGIN      
        1 cgggctcgat gtccaggcgc gcgaagccct gtggaaagtg gtgcgcgagc tgcgccacga
       61 aggctgctcc atcgtgctca ccacgcacta tctcgaagaa gccgaggcgc tcgccgaccg
      121 cgtcgcggtc atggcgcgcg gccggctcat cgccggtggc agcgtcaacg aaatccgcgc
      181 gcacgtgacg cgcaaacagg tgagctgcgt gacccgactc accgccgaca cggttagcgc
      241 ctggcccgag gtcgtacaag tggaattgga gcgcgaccgc ctgaagatca ccacgcgcaa
      301 cgccgaagcg ctgctcgtgc agctgttccg cgaggatccg cagctttccg acatcgaagt
      361 caagcgcgcc gggctcgccg aagcgttcac cgagctcacc aacgactcca agaaggaggc
      421 tgcatgaacg ccgtcagcaa catgtcaggc tcgcgcctgt gggccgcgta ccttggtgag
      481 atgcgcttcg agttcatcaa gtcactgcgc acgccggctt tcgccgtgcc gacgctatta
      541 tttccgattc tcttctatgt gatgttcggc atctttttcg gaagcatgcg tggcaacagt
      601 ggccaggcgg cgtacctgtt cgccacctat ggcgtcttcg gcgccatggg accggggttg
      661 ttcggctttg gcgtgtcgct cgccatcgag cgcgagcaag gcctgctcac gctcaagcag
      721 gcgctgccgc agcctcccgg cgcctatcta ctggcgcgtg ccgccatggc catgctgttc
      781 gtcggaatca tcgcgctgat gctcacgtcg atggccgtgt tcgtcggcca cgtaccgctg
      841 acgttttcgc agggcatccg cctgttcgcg atcgatgtgt tcggcgcttt gcctttctgc
      901 gccatcggca tgtgggtcgg ctcaatggtt tcaggcgcag cttcgccggc catcgtgaac
      961 ctgatcttcc tgcccatggc cttcctgtcg ggcctgtggg taccactgca gttcatgccc
     1021 aaggtcctga tcgacttcgc gccgatctgg ccctcctacc atcttgcaca aatggcactg
     1081 agcaccgtgg gggaaaagag cagcggcacc accatgggtc acgtggcggt tctcgccggc
     1141 gtcacgctgg tgttcttcct gctagccatg cgccggatgc acggaagcgg attccgattg
     1201 ttaggcgccc agccgaaacg cacgctggcg atcgtcgccg gcgtctgcgc ggcggtgatt
     1261 gccttgtcgt tgtcgggcgt gttcggtggc agggaaacgg acaatgcctc ccctggcacc
     1321 gcttccgttg ccaccggcac gcccgccgcg gaagccgctg ccggtatcgc agctcctcag
     1381 gtggtcgtgc tgtcgaattt cgacgccgga tccgctgcga cggcctatgg cgtcggctgg
     1441 agcgcggccg gagacgacat gcgcggggga aattcgcacg ccagccagcg cgtggtcgat
     1501 gacggcgccg gcggcagcaa gggcgcgctc gaagtgacgg gcgcggtcgg cgaccagatc
     1561 cagtacccat tcgccggcac gatgttcttt ccggagggcc cgcccatgca gggcctcatg
     1621 gactactccg gcaagaagac cctctctttc caggcacgcg gcgatggccg gcgctacatg
     1681 ctcatggtga tctcggggtt ggccgtggaa ggcattccgc tcatggtcga cttcgagacg
     1741 gggcctgagt ggcacgaagt gcggctggaa atcgccaaac tcggcgccgc ggactggaag
     1801 cgggtgcgcg ccattggcgt cggcaccatg ggacccgcag gcgcattccg cttccagatt
     1861 gatgatgtgc gcgtggagta agatcgcacc gggaaccgac tacatgcgcc tcaaacaact
     1921 gctgcaaaac atccgggagc cacccccgac ctcgatggtc ggccgcatga tcggcgtcga
     1981 cggccgcgtc atgcgcaaca tgccgttcgt gatcctgttc aatctggcct ggttgttcgt
     2041 ctaccccgcc atcgcaggcg caccgttcgt gcgcgtcatc ctgcccacca tcatcagcct
     2101 gccgctgttc gtctatctgc acttgtgctc gtactactac ggtggctcca acgaagtgcg
     2161 cctgcggtac atcgccggca tcttcctgct ggggctggcg ctgacctatt tcaaccttgc
     2221 cgcggtcggc tacctgatct tcggctattt cagtgttgct ttcgccgtgc ccacccgtat
     2281 cgcgtggaag gtgatattcg gtgtctgcgc cgcctacgtc atcgagatca tcctgctggg
     2341 tcacaacacc cagactgtgc tcagctgcac cttgcctgcg gcgttgctcg gggtctcggc
     2401 tctctacgcc gcctacacca cgcatcagca gatggcattg cgccggagca acgaggaaat
     2461 cctgcgcctg gcgacgatgg cagaacgcga gcgcatcggc cgcgacctgc atgacctgct
     2521 gggccacacg ctgtcggtgg tcgcattgaa atcggagctg gcacgcaagc tcatcgaccg
     2581 cgatctcgaa gccgcgcgca aggaaatcag cgaggtcgag cgcgtggcgc gcgacgcact
     2641 ctctcaggtt cgcaacgcgg tctcgggcat ccgaagcacc gcgctttctg ctgaactggt
     2701 cgcggcaacc gcccttctcg aagcgcaggg cgtcaaggtg aagtgtgaaa ccgagaacgt
     2761 gaagctgccg cacgaccggg agacggcgct ggcactgagc ctgcgcgaag ccgccaccaa
     2821 tatccgccgg cattcgggcg cgtccggcgt gacgatccgc gtgaggaaag agtcatcggc
     2881 agtcgtcgtc gaagtcgcgg acgacggccg cggcggccgc atcgtgcccg gcaatggatt
     2941 gaacggaatg cgcgagcgcc tcgacaccgt aggaggcacg ctctcactca ccgccaacga
     3001 gaacggcggc acgctgctgc gcgcgtcggt ccccgttgcc gcatgaagag ggccgcatga
     3061 tccggatcct gctggccgaa gatcaggcca tggtgcgcgg tgcgctcggc gcactgctgc
     3121 gcatggaacg cgatatcgaa gtgctcggcg acgccgccga tggcatgcag gcgctctccg
     3181 aatgccggcg gctgaagccg gacatcgtgg tcaccgacat cgagatgccc ggcatgaccg
     3241 gcatcgagct ggcgcagaag atccaggccg acggcctgtc atgcaaggtg gtcatcgtca
     3301 cgacgttcgc gcgccagggc tacttgcggc gcgcgctgga cgccggggtc aagggctacc
     3361 tgctcaagga ttcaccggcc gagaagctgg ctgaagcgct acgcaatgtg caccgtggcg
     3421 gcaaggcgat cgatcccgca ctggcggtgg aagcctggaa tgagtcggat ccgctcaacg
     3481 atcgcgagcg ccagacgctg cggctgtcgg gcgaaggact gtccgcgccg cagatcgccg
     3541 agcagctcgg attgtccgcc ggcaccgtgc ggaactatct atccgaggcc atcggcaagc
     3601 tcggcgccaa caatcgtatc gatgcgtacc gtctcgcgcg ccagcgcggc tggctgtaag
     3661 aggtgccggg ccgggaaacc gggaaaactc tgaaggtcgc cgggtgtgaa aaggcgttca
     3721 ccgagttttc ccggtttccc ggcccggcac cgtcctacgg caccgtccta tccgcggccg
     3781 atatagggca tcttggtcgc gagaatggtc atgaactgaa cgttcgcctc gggcggtagt
     3841 ttggccatct gcagcaccgc gttggcgaca tgacttacgt ccatcatggg ctccgggtcc
     3901 ggcaggcgac tgccgagctg ggtcagtgca ttgccgatgt cgatctgacc gcaggcgatg
     3961 tcgtacttgc gcccgtccaa tgaagtcgcc ttcgtcaatc ccgtgatcgc gtgcttcgac
     4021 gccgtgtacg gcgcggaatt cggccgcggc gcatgcgctg atatcgaccc gttattgatg
     4081 atgcggccgc cgcgcggcgt ctgatccttc atcagccgga atgcctgctg cgtgcacaga
     4141 aacgcgccgg tgagattggt gtcgatgact tcgcgccact tctcataggg catgtcttcg
     4201 agcggcagga atggcgcatt caatccggcg ttgttgaaca gcacgtctag gcggccgaac
     4261 ttttcgtgcg tggcgtcgaa caacgcgcgc acctcgtcag gtttcgtcac atccgtcacg
     4321 acgcacaacg actgcgctgc atcgcccgcc tctcgcgcca cggcttcgag cttgtcggcg
     4381 cgacgccccg cgagcaccac gcgaaaaccg tcgcgcagaa aggcgattgc cgtggccttg
     4441 cctatgccgc tgcccgcacc cgttatcaac gccacccgct tttccgtcat gcctggccct
     4501 tatgtttacg cgtcgccccg ccgcaacagg agctccgcga atgcgccgtt ttcgtcgacc
     4561 cgcagctcca tttctatggg ctggcagcag acctggcaat cttcgacata actgcacaga
     4621 ccggcgctgg catccacgct agtctcgaat tcttcgccgc aataggggca gctgacgcgc
     4681 gtgaattcgc tcgaggcggc actgtcaaat gtggcggcat cgccagcaat gacgggctcg
     4741 agcccgtaga gcgcatccac ttcggcgtcg gtcagcggtt tttctggaga gttcatgttc
     4801 actcgcggtt cgatgatggc acgactcgcg ctgctcgtcg gcatcagcct cgtgacgatg
     4861 ggcaacttcg ccttctgcag tttcaattcc ggcccgggcg gcggtaccgg tttgcccggt
     4921 gggcccgatg ggcccggcgg caacgacagc ttccggagca ctctcgtcct gcgcaacgtg
     4981 tccggtgtgg agacctcgag cttcgtgttc ggcgaggcca tacgcttcga tttcgccgtc
     5041 gagaatcgaa ccgcccgcca gttgcgcctc tccttccccg acgcccagac gacggactac
     5101 ctcgtcgtga accagggcac cacccagatc cgctggatgt ggtccgaggg ccagtccttc
     5161 gcgcaggtgg ccaccgagct cgtgttcgaa ccctattcca ccaggtcctt ctcggtgacg
     5221 tggaatggca acctcagcga cggtacgaac ctgccgccgg gcgactatca ggcgcgcgga
     5281 ctcatggttt tcgacggtta cgagaccaac ccgctggcgc ccagcgaact cgctgcgccg
     5341 ttggtgccat tcaccgttcg ctgaaaacat tcccggattc atgcaccccg gcgggcgcct
     5401 gcctcgtata gatggcagac acgcaaatca ggagcccgcc gtgagattca gagccttttc
     5461 tgccgccttg gccgccgcca ccgccctcgc cctgcccgtt gccgggtttg gggtcgagcc
     5521 cgaactcaat attccgaatt tcagtcatct gaagcacaaa gccaccgaat cggtggatgt
     5581 gacgctcgat ggtttcctga tgcgcatcgc ccagaaattc gctgcatccc atgccgccga
     5641 agaggcgcat gacgaggcta tttcgatact ccaggacata aagtccgtgc gcgtgcgcaa
     5701 cttcactttc gactccgaca acgcgtattc catggcggat atcgacagtg tccgcaagca
     5761 attgaaggcg cccggctgga gcgcgctggt gcaggtccac agacgggata acgaggacgt
     5821 ggatgtctac gtctgcctcg aggacgacaa ggtcaaaggt ctggccgtga tcgccagcga
     5881 gccgcgcgag ttcaccatcg tgaacatcgt cgggagtatc gatctcgata agatctcgca
     5941 actcgaagga gaattcggca ttccccgaat gagtgaacaa gttgagtgaa caagttgagt
     6001 gagaacgagt gacaagaccc cgtgagcctc tccgccgccc aaccgatgcc tgattcgctg
     6061 ctcgcgcagg cgcgtgccgg agaccgcgac gcgttcgcca cgctggttcg ggcacaccag
     6121 gggtcggtgt tcagtattgg cctgcgcatg ctcagccggc gcgacgcggc cgaagacctg
     6181 gcgcaggacg tgttcctgca gctgtaccgc aagctcgatt ccatcgagtc gctcgaacac
     6241 ctgggattct ggctgcgtcg cgtggcggcg aacctggcca tcgactggct gcggcgcctg
     6301 ccctacagcg ccacgcggcc actcgaagaa ggcgcggaag tcgcggccca ggaaaaggac
     6361 caggatctgc tcatgagccg cgaactcatg cggttgttag gcgagctggc gccgcacccg
     6421 cgcgcggtga tgctgctgcg ctaccaggaa gaccgtgacg tcgccgagat cgccgcgatg
     6481 ctcgacatgc ccgtgaatac cgtgaagagc cacatcaagc gctcgttgac cgcgctgcgc
     6541 ggccagatga tcggcgcgaa gctggtcgat tgcgaggaac tgccatgaac gaaaattttg
     6601 ccaatgaatt tgaaagggaa cttcgccggc gcctgcggcc cgtggaggcc cccgaaggct
     6661 ttgccgaaag gctcatggcc gcgttgcctc cggagaagaa atccacggtg gttgcgttca
     6721 tccccaaggc ctcgcgcccg gt
//
LOCUS       DASQJS010000380         8052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3693904, whole genome shotgun sequence.
ACCESSION   DASQJS010000380 DASQJS010000000
VERSION     DASQJS010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3693904"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VEW08_14985"
     CDS             <1..181
                     /locus_tag="VEW08_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42096.1"
                     /translation="GTMSKKQFDDLFDEAQALSEAGDSDGSLAKYLAALGLEPENPNV
                     LYKARVARSWNKDRR"
     gene            219..728
                     /locus_tag="VEW08_14990"
     CDS             219..728
                     /locus_tag="VEW08_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42097.1"
                     /translation="MRLNPDADGEVVWATRLCPVRARINNIPFPESGFAYGDVVLNDG
                     AAVGYRLDSHGRETAVFNVLEMFEPGTFSTYRVDLVAESVAHVEAFEKLCDARDLQSE
                     DWNSSVQTICKACSEGRPHDKHDHTPVKAPWKPERQLAVAAKDENDVKAVIDAWDGNV
                     TEWGPALKR"
     gene            complement(739..1527)
                     /locus_tag="VEW08_14995"
     CDS             complement(739..1527)
                     /locus_tag="VEW08_14995"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HYJ42098.1"
                     /translation="MPSGKKRRLTFALVVAYLPLALAYLSFSGQLAARDWRTASREPA
                     GLAPDPATTQEAVVQVYSARAVSWRGWFGVHTWIAVKPSNAKEFMVHEVMGWRLKRTG
                     TALVTRNRPADGYWYGSRPELLGDIRGPGVDAIIQRIETAVSEYPYPNSYHVWPGPNS
                     NTFVAFVLRRVPELRVDMPPTAIGKDYLGWRSVNKTPSGTGGQVNLLGVLGVAAGIEE
                     GLEVNLLGLNFGVDPKSLSIKLPIVGRLGPKNSPKPTTIAAGET"
     gene            1584..2114
                     /locus_tag="VEW08_15000"
     CDS             1584..2114
                     /locus_tag="VEW08_15000"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ42099.1"
                     /translation="MRKPDLDSLRLETPRLVLRPLRMEDFDAWAAMHDDEITMKYIGG
                     RQPRATAWRMFMCMCGAWSMTGIAMFSVLEKASGKWVGRLGPWYPDGWPDHEVGWGIA
                     REHWGKGYATEGAAATMDYAFDVLGWTNVIHCIEENNAASQGVAKRLGSQLLRRVKMP
                     APYENFVVDAWGQTRE"
     gene            2269..2946
                     /locus_tag="VEW08_15005"
     CDS             2269..2946
                     /locus_tag="VEW08_15005"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYJ42100.1"
                     /translation="MARPPRIIVADWCYHFLNRANRKAEIFHEPSDYDAFIGLVGQAQ
                     LRFSVPILAYCLMPNHVHFVVRPGSGEDLTRWAHWLFTSHAHRYRTKHDSSGHVWQGR
                     FKTFAIQGDQHLLTVLRYVERNALAGKLVDRAEEWRWSSLRWRTHTSTPVTLGVCPVD
                     LPSYWVDYVNQPQTAVELSEIRTCVSRQRPFGAEEWVSEATDRLQNHQSLDPIGRPRK
                     RKPGTVS"
     gene            complement(2943..3833)
                     /locus_tag="VEW08_15010"
     CDS             complement(2943..3833)
                     /locus_tag="VEW08_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HYJ42101.1"
                     /translation="MSSAQGSVMGSALGSKWLVSRPMNSRSLIVYRGRAGDRNTRGTA
                     GAQMLGALLARRYGLDTTFVSTPHAPLPSTAWNEQLAAARGDLHAFADALRESLSHGH
                     RAIVAMGRCAAGLATIPVIAERHPDACIVWFDAHGDSNLPSSNAVPYLGGMVLTGAAG
                     HWDSGLGAGLKLSNVVLVGARDLDPHEKELIATGQLKVVEIGPNLPARLAAAVGMRDV
                     YVHLDCDVLEPGIVPIEYQVSDGLSLDDLRACATLLAKRKVIGIEIAEFESEWLDGRP
                     ASPDRLVDAIAPLLGSGTVF"
     gene            complement(3826..4140)
                     /locus_tag="VEW08_15015"
     CDS             complement(3826..4140)
                     /locus_tag="VEW08_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42102.1"
                     /translation="MTTKRSKYDPLATSRLSRWLVLRDMFHNVLDATELPPGSDLRQS
                     FADGLAALEREGWQNEGASGAMVFVRRGGERRLLSVHEHDPHEPLPPGHGVLSGRCAT
                     CE"
     gene            4516..5793
                     /locus_tag="VEW08_15020"
     CDS             4516..5793
                     /locus_tag="VEW08_15020"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ42103.1"
                     /translation="MNKSWTLLLSGALLVELSGVTASAQVVGTSANSFAIRDVRVFDG
                     KREFSRTNVVVIEGRVAAIGQRIAPPAGLAVIEGRGKTLLPGFIDSHVHVFPGAQADA
                     LRFGVTAELDMFNLSHEFARWSAQRESLNAVFEADTWSAGTGVTVPGGHPNQWVPEDM
                     PRLSGADGAPAFIEARIAEGSNYIKLIVEDNAALDPANPLHTLSRPELCASVAAARAH
                     GRLVVAHVTNQANARMAIECGVNGLAHTVVDAPMSAELVRLAKKRGVFAVSTLSLLAA
                     ESGASPGDLPASADVRLLSASQRGSLNRTTPLVHPGQLAIAQESVRRMHDAGVIVLAG
                     TDAPAPGTAHGVSMHREMALLVQAGLTPVEALAAATSVPAKVFGLGDRGRIAEGNRAD
                     LLLVDGDPTVEIAATLRIDRIWKNGYPVDRAVP"
     gene            complement(5838..6485)
                     /locus_tag="VEW08_15025"
     CDS             complement(5838..6485)
                     /locus_tag="VEW08_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ42104.1"
                     /translation="MNAAVKAPVHKIRVILADDHPVVRDGLAAIVNQQPDMEVVAEAG
                     DGAEALELYELHKPDVMVLDLRMPKLDGAAVVQKVLEKHPKACLLIMTTYDGDEDIFR
                     SLSQGAKGYLLKDAPRQEILSAIRAVADDRPYTSSGVAAKALQRMSKPSLTTRELDVL
                     QLVAEGRSNKDIARRLAITEGTAKTHVKAILGKLDAISRTEAVAVAHKRGLIRLS"
     gene            complement(6469..>8052)
                     /locus_tag="VEW08_15030"
     CDS             complement(6469..>8052)
                     /locus_tag="VEW08_15030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HYJ42105.1"
                     /translation="WVTESLSDAVCPMSSGECTDPWNEPRSDQFCTMLQAGKSVVFCP
                     DAARAIEGVSITSDKARTSAIVPILVDGEYVGVIGFDDWQRVREFDSSIVSALEIAAG
                     LVGAALHRQRLVDTMRREREMAAEERLSSATRANALLRTNLTKLARDADPQDFVSNIL
                     IDATRQLGAVAAYTIGFDKAANLWKTFIHVEGGKVAPPPYQATATPEETRLSEVLALF
                     TEPSFYQVQPLDRIQWPGAAEWHAREGHKSVYLLPVVVGEQTVGCIGLAFLQDEALNA
                     ERAELALAVAHQITLCIGLKKLGMAAKDAAVLAERNRIGQEIHDGLAQGFTGILMQLG
                     AAEEAMHECARNSPLPSILTRIHDLAKQGLADARRSVMALRPDQTKRVGLELALRQLA
                     ERATVEGRAICSFEGAVIGDGLVPERQHELLRIATEAVSNALRHAHPSTVRIVLLEEE
                     AHWTMSVIDDGRGMTLSPELYAQQGYGLNNMRERAGAIGGQFQIESKPGEGTRVVVNL
                     PRPHLTNVSKLKAQNERRG"
BASE COUNT         1388 a         2431 c         2738 g         1495 t
ORIGIN      
        1 agggacgatg agtaaaaagc agttcgacga cctgttcgat gaagcgcagg cgttgtcgga
       61 agccggggac agcgatggtt cgctcgcgaa gtacctcgcg gcgctcggtc tcgagcccga
      121 aaatcccaac gttctctaca aggcgcgtgt ggcgcggtct tggaataaag atcgaaggtg
      181 aagagggccc gatcgagggc aggttcgggc atgcgcccgt gcgcctgaac cctgacgccg
      241 atggcgaagt cgtgtgggca acgagactgt gtccggtacg cgcccgcatc aacaacattc
      301 catttccgga atccggcttc gcgtacggag acgtggtgct caatgacggc gcagccgtgg
      361 gttatcggct cgacagccac ggtcgcgaaa cggcggtgtt caacgtgctc gaaatgttcg
      421 agccaggaac tttctcgacc tatcgagtcg acctggtggc cgaatccgtc gcgcacgtcg
      481 aagcattcga gaaactgtgt gatgcacgtg acctgcagtc cgaggactgg aacagcagcg
      541 tgcagacgat ctgcaaggcc tgcagcgagg gccgccctca cgacaagcac gaccacacgc
      601 ccgtcaaagc gccgtggaag cccgagcggc aactcgccgt ggccgcaaag gatgaaaatg
      661 acgtgaaagc tgtgatcgac gcgtgggacg gaaatgtgac ggagtggggt cctgcgttga
      721 aacgctgacg cgtcgcggtc aagtctcgcc ggccgcgatg gtcgtaggct tcggcgaatt
      781 cttcggcccc agcctgccga cgatgggtag tttgatggac aggctcttgg ggtccacgcc
      841 gaaattgagc ccgagcagat tcacttcgag gccttcttcg attcccgcag ccacgccaag
      901 cacgccgagc aggttcacct ggccgcccgt gccgctcggc gtcttgttga ctgaacgcca
      961 gccgagatag tccttgccga tggcggtggg cggcatgtcc acgcgtaatt ccggcacacg
     1021 gcgcagcacg aaggccacga aggtgttgga gttcgggccc ggccaaacgt gatagctgtt
     1081 gggataggga tattcgctca cggcggtttc gatgcgctgg atgatggcgt cgacgcccgg
     1141 gccgcggatg tcgcccagca actcgggccg gctgccatac cagtagccgt ccgcgggacg
     1201 attgcgcgta accagcgcag tgccggtgcg cttgagccgc caccccatga cctcgtgcac
     1261 catgaattcc ttcgcattgc tgggtttcac ggcgatccag gtgtgcacgc cgaaccagcc
     1321 gcgccagctc acggcgcgcg cggaatagac ctggacgact gcttcctgcg tggtggcagg
     1381 atcgggcgcg agcccggctg gttcacgcga cgccgtgcgc cagtcgcgtg cggcgagctg
     1441 gccgctgaaa ctgaggtatg ccaaggccag gggcaaatac gccacgacca gtgcaaaggt
     1501 gaggcggcgc ttttttccgg atggcatagg gaactgacac tagcagtgtt ggccgagttc
     1561 cgggccgggc tatgcttgga tccatgagaa agcccgacct tgattcgctg cgactggaaa
     1621 ccccgcgcct ggtcctgcgt cctttgcgaa tggaagactt cgacgcgtgg gccgccatgc
     1681 acgacgacga aatcacgatg aagtacatcg gtggccgcca gccgcgcgcg acggcgtggc
     1741 gcatgttcat gtgcatgtgt ggtgcgtggt ccatgaccgg catcgccatg ttctcggtcc
     1801 tcgagaaagc gagtggcaag tgggtggggc gcctggggcc gtggtatccg gatggctggc
     1861 ccgaccacga ggtgggctgg ggcatcgccc gcgagcattg gggcaagggt tacgccaccg
     1921 agggcgccgc ggcgacgatg gactacgcgt tcgacgtgct cggctggacc aacgtcatcc
     1981 attgcatcga agaaaacaac gccgcctcgc agggtgtggc gaagcgcctc ggttcacagc
     2041 tcctgcgccg ggtgaagatg cccgcgccct acgagaattt cgtggtggac gcctggggac
     2101 agacgcgcga gtagtggaag aaaagggggt caggcaccgt ttcctagcaa acggtgccag
     2161 gcaccgattt ggcgctgtct tgtcattttc caccccactc aactattcgg gcgcatgcgt
     2221 ccgtttttcc gctcctgtcc gtcggggcgt gcgggctaga ttcttcgtat ggctcgccca
     2281 ccaagaatca tcgtcgcgga ctggtgctat cacttcctga atcgcgccaa tagaaaggct
     2341 gaaattttcc acgagccttc ggactacgat gcattcatcg ggctcgtcgg ccaagcccag
     2401 cttcgctttt cggtacccat ccttgcgtac tgcctgatgc cgaatcacgt gcatttcgtc
     2461 gttcgacctg gttccggcga ggatctcacg cgatgggcgc actggctgtt cacatctcat
     2521 gcacatcgct atcgcacgaa gcacgattcg agcggtcatg tctggcaagg ccgtttcaag
     2581 acgttcgcaa ttcagggcga ccagcatttg ctcacagtgc tgcgctatgt cgagcgcaat
     2641 gcgttggcgg gaaaactcgt ggatcgggcg gaagagtggc gatggagcag ccttcgctgg
     2701 cgaacccata cgtcgacgcc cgtgacactc ggcgtttgcc ctgtcgacct gccgagctac
     2761 tgggttgatt acgtgaatca gccccagacg gcggtggaac tctctgagat caggacctgc
     2821 gtcagccggc agcggccttt tggtgccgag gagtgggtga gcgaagcgac tgatcggctt
     2881 cagaaccatc aatcgctgga tccgatcggg cgcccgcgca aaaggaagcc tggcaccgtt
     2941 tcctagaaaa cggtgcctga ccccaatagc ggagcgatgg cgtcgaccag ccggtcgggc
     3001 gacgcggggc gaccatcgag ccattcggat tcgaactccg cgatttcgat gccgatgacc
     3061 ttgcgcttcg ccagcaacgt tgcgcaggcg cgcaggtcgt cgagcgagag tccgtcggaa
     3121 acctggtatt cgatgggaac gattccgggc tcgagcacat cgcagtcgag gtgcacgtag
     3181 acgtcgcgca tgcccaccgc ggcggcgagc cgcgcgggga ggttgggtcc gatttccact
     3241 accttcagct gcccggtggc gatgagttct ttttcatgcg ggtcgaggtc gcgggcgccg
     3301 accagcacga cgttcgacag cttcaggccg gcgcccaggc cgctgtccca gtggccggcc
     3361 gcgccggtga gcaccatgcc gcccaggtag ggcacggcgt tggagctggg caggttcgaa
     3421 tccccatgcg cgtcgaacca cacgatgcag gcgtccgggt ggcgttccgc aatcacgggg
     3481 atggtggcga gccccgcggc gcaccggccc atggcgacga tggcgcggtg gccgtggctc
     3541 aacgattcgc gcagagcgtc ggcgaacgcg tgcaggtcac cgcgcgccgc ggcgagctgt
     3601 tcgttccagg cggtgctcgg cagcggcgca tgcggcgtgc tcacgaacgt ggtgtcgagg
     3661 ccgtagcggc gcgccagcaa cgcgccgagc atctgcgcgc ccgcggtgcc gcgggtgttg
     3721 cggtccccgg cgcgtccgcg gtagacgatg agtgaacggg aattcatcgg ccgcgagact
     3781 aaccacttcg agcccaatgc gctacccatg acgctgccct gggcactact cacacgtggc
     3841 acacctaccg gagagtactc catgccctgg gggaagcggc tcgtgcggat cgtgctcgtg
     3901 caccgacagc aggcggcgtt cgccgccacg cctaacaaag accatcgcgc cgctcgcgcc
     3961 ttcgttctgc cagccctcgc gttcgagcgc ggcgaggccg tcggcgaaac tctggcgcag
     4021 gtcgctgccg ggcggtagtt cggtggcgtc gagcacgttg tggaacatgt cgcggagcac
     4081 gagccagcgt gagagccggg acgtggccag ggggtcgtac ttgcttcgtt tggttgtcat
     4141 ggagcgaacg ttatcgagcg gcgttcgcag tcgtccattc agtagtccgc ggcgcagcga
     4201 actgtatgaa ttggaaaaaa ttacaaaaga gaggagggta agtgcgcgtc gctgcgcaag
     4261 atttgggcga tggcggcaga aaaaaacagg ccgcgcttta gcgcggcctg tctgcggcgg
     4321 tcagttcgca tttcataaga tgctggttgc cgccacgcgt agcttcacgt cggtcaatcg
     4381 ggaggattgc ttcatgaacc tacgatttca gtattcacga tacggccagg cgcgcgaagt
     4441 cgaatcgttc tgaaacggcg gtcataaatc tcctacaaca tgcgacgaaa gccagcctct
     4501 gcgcatccat gacggatgaa caaatcttgg actctgctac tgtcgggcgc gttgctggtc
     4561 gaattgtcgg gcgtaacggc gtccgcgcag gttgtcggca ccagcgccaa cagtttcgcg
     4621 atccgtgacg tccgggtgtt cgacgggaag agagagttct ctcgaacgaa tgtggtggtc
     4681 atcgagggtc gcgtcgcggc gatcggccag cgtattgcgc cgccggctgg tctggccgtc
     4741 atcgagggac gcggaaagac gttgctgcca gggttcatcg attcgcacgt gcacgtattc
     4801 cccggtgccc aggcagatgc tttgcgtttc ggtgtcaccg ccgagctcga catgttcaac
     4861 ctgagtcacg agtttgcgcg ctggagtgcg cagcgcgagt cgctaaatgc cgtgttcgag
     4921 gcggatacat ggtcggccgg cacgggcgtg accgtcccgg gcggtcaccc gaaccaatgg
     4981 gtgccggagg acatgccacg tctgagcggc gccgatggcg cgccggcgtt catcgaggca
     5041 cgtatcgcgg aaggctccaa ctacatcaag ctgatcgtcg aggacaatgc ggcgctcgat
     5101 ccggcgaatc cgctacatac cttgtcgcgg cccgagttgt gcgccagcgt cgctgcggcg
     5161 cgggcgcacg gcaggctggt agtcgcgcac gtcacgaacc aggccaatgc gcgcatggcc
     5221 atcgaatgtg gcgtgaacgg actcgcacac acagttgtcg atgcgccgat gagcgccgag
     5281 ctggtgcggc tcgcgaaaaa gcgcggggtc tttgcggtgt cgacattgtc attgctggcg
     5341 gctgagtccg gcgcttcgcc tggagatctc ccggccagcg cggatgtgcg actgctgtca
     5401 gcttctcaac gcggctcgct gaacaggacg acgcccctgg tccatcctgg gcagcttgcg
     5461 attgcacagg aaagcgtgcg tcgcatgcac gacgctggcg taatcgtcct cgcaggaacc
     5521 gacgcgccgg cgccaggtac ggcacatggc gtctcgatgc atcgtgagat ggcattgctg
     5581 gtgcaggccg ggctcacgcc ggtggaggcg ctggcggcgg caacctccgt gcccgcgaag
     5641 gtttttgggc tcggtgatcg cgggcgtatt gccgagggta atcgcgctga tctgctgctc
     5701 gtcgacggcg atcccacggt cgaaattgcg gcgacgttgc gcatcgatcg aatctggaag
     5761 aacgggtatc ccgtcgaccg cgcggtaccc tgatcaggtg cggccccttt tgacaggaaa
     5821 tggggagtgt ccccatttca gctcaggcgg atgagaccac gcttgtgcgc gacggccacg
     5881 gcctcggtgc ggctgatggc gtctaacttc ccgagaatcg ccttgacgtg ggtcttggcg
     5941 gtgccttcgg tgatcgcgag tcggcgcgcg atgtccttgt tgctgcggcc ctcggccacg
     6001 agttgcagca cgtcgagctc gcgcgtggtc aggctgggct tggacatgcg ctgcagcgcc
     6061 ttggccgcga cgcccgaaga cgtgtacggg cggtcatcgg cgacggcgcg gatcgccgac
     6121 aggatttcct gtcgcggcgc gtccttcagc aggtagcctt tcgcgccctg gctcaacgaa
     6181 cggaagatgt cttcgtcgcc gtcgtacgtg gtcatgatga gcaggcaggc cttggggtgt
     6241 ttctccagga ccttctgcac gacggcggcg ccgtcgagct tgggcattcg caggtcgagc
     6301 accatcacat cgggcttgtg cagttcgtac agttccaggg cctcggcgcc gtcgccggct
     6361 tcggcgacca cttccatgtc cggctgctgg ttgacgatgg ccgcgagtcc gtcgcgcacc
     6421 accggatggt cgtcggcgag gatcacgcga atcttgtgga ccggcgcttt aaccgcggcg
     6481 ttcattctgt gccttgagtt tgctgacatt ggtgaggtgg ggacgcggca ggttgacgac
     6541 gactcgcgtg ccttcaccgg gcttgctttc gatctggaac tggccgccga tggcgccggc
     6601 gcgttcacgc atgttgttca ggccataacc ctgttgcgcg tacagctcgg ggctcagcgt
     6661 catgccgcgg ccgtcgtcga tcacgctcat ggtccagtgc gcctcttcct cgagcagcac
     6721 gatgcggacc gtgctcgggt gcgcatggcg cagtgcgttg ctcaccgcct ccgtggcgat
     6781 tcgcagcagt tcgtgctggc gctcggggac gaggccatcg ccgatgacag cgccttcgaa
     6841 actgcagatg gcgcgacctt cgacggtggc gcgttcggcg agctggcgca gtgcgagctc
     6901 gaggcccacg cgcttggtct gatccggtcg caacgccatc accgagcggc gcgcgtcggc
     6961 aaggccctgt ttggcgagat cgtggatgcg cgtgaggatg gaaggcagcg gcgagttgcg
     7021 cgcgcattcg tgcatggctt cttcggccgc gcccagctgc atgaggatgc cggtgaaacc
     7081 ctgggcgagt ccgtcatgga tttcctggcc gatccggttg cgttcggcaa gaacggcggc
     7141 gtccttcgcg gccatgccga gcttcttgag cccgatgcac agcgtgatct ggtgcgcgac
     7201 cgcaagcgcg agttcggcgc gctcggcgtt caacgcctca tcctggagga acgccaggcc
     7261 gatgcagccc acggtctgct cgccgaccac cacgggaagt agatagacgc tcttgtggcc
     7321 ttcgcgcgcg tgccactcgg cagcgccggg ccactggatg cggtcgagcg gctgcacctg
     7381 gtagaacgat ggttcggtga agagcgcgag cacttcgctc aggcgggtct cctccggcgt
     7441 agccgtggcc tggtaaggtg gcggcgcgac cttgccgccc tcgacgtgga tgaaagtctt
     7501 ccagagattg gcggccttgt cgaagccgat ggtatacgcc gccacggcac cgagctggcg
     7561 cgtggcgtcg atgaggatgt tgctgacgaa atcctgcggg tcggcatcgc gcgcgagctt
     7621 ggtgagattc gtgcgcagca gcgcgttggc ccgcgtcgcg gatgacaggc gttcttcggc
     7681 ggccatctcg cgttcacgcc gcatggtgtc gacgaggcgc tggcgatgca gggcggcgcc
     7741 gaccaggccc gcggcaatct cgagggccga gacgatggaa gaatcgaact cgcgtacgcg
     7801 ctgccagtca tcgaagccga tcacaccgac gtattcgcca tccacgagga tgggcacgat
     7861 cgccgaggtg cgggccttgt cgctcgtgat gctcacgcct tcgatggcgc gcgccgcgtc
     7921 gggacagaac accacgctct tgccggcctg gagcatggtg cagaactgat cggagcgcgg
     7981 ctcattccac gggtccgtgc attcgccgga ggacatgggg cacacggcgt cgctcaggga
     8041 ttcggtgacc ca
//
LOCUS       DASQJS010000381         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_245241, whole genome shotgun sequence.
ACCESSION   DASQJS010000381 DASQJS010000000
VERSION     DASQJS010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245241"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..840
                     /locus_tag="VEW08_15035"
     CDS             133..840
                     /locus_tag="VEW08_15035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HYJ42106.1"
                     /translation="MLSKPQNCSEGVIRLHSRIAQACLAASRSVDSVTLLAVSKGQSS
                     AAINGAASAGIEDFGENFLQESLPKIETLAGRELTWHFIGRLQANKTRPVAENFAWVH
                     TVDRLKIAERLSAQRPDHLPPLNLCLQLHVGGEASKGGIEAADVAPLAAAVRELPRLR
                     LRGLMCMPPAEDSIPRQRQWFRETRQVFDYLNERGLGLDTLSMGTSGDFEAAIAEGAT
                     MVRIGTAIFGPRDSTAP"
     gene            865..1692
                     /gene="proC"
                     /locus_tag="VEW08_15040"
     CDS             865..1692
                     /gene="proC"
                     /locus_tag="VEW08_15040"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HYJ42107.1"
                     /translation="MNINAAFVGGGNMGGALIRGLIARGLSPQNISVGEANQQRRIAL
                     ADELGVHVTADNREAVASADVVVLAVKPQDMAGTVQALADVFAQRPPLVLSIAAGIRI
                     ADIVSWCGPGVAVVRAMPNRPALNSAGATAMYAPVSLSEAHRGLAAAVLGAVGTAVWV
                     HEEDALDVVTALSGSGPAYFFLLAELMTDAAVNLGLDRASAQELSIQTLFGSGRMARD
                     SDGDLARLRAEVTSKGGTTEAALRSFDSANLRGIVAAAISAATDRSRELAQAFGNKK"
     gene            1695..2237
                     /locus_tag="VEW08_15045"
     CDS             1695..2237
                     /locus_tag="VEW08_15045"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HYJ42108.1"
                     /translation="MNALYYLVDTLTSLYLGVLILRLIMQIARANFRNPFAEAVLRLT
                     NPLIMPLRRVLPPVGKMDSATVLAIVLFAIFKVVLLSLLFGGGLLSLVAMAIVVLRTL
                     VLAVLWLYFILIFFYGIAGFLMQGGSSPVYEVLAYVCDPILNRIRKIIPSLVAGLDLS
                     FLWALIAIQALIILIGEIRL"
     gene            complement(2268..>2590)
                     /locus_tag="VEW08_15050"
     CDS             complement(2268..>2590)
                     /locus_tag="VEW08_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42109.1"
                     /translation="SLQIVATISDFVGAPPFFAITRLLVKKLQRKKYPRRAVRRATYH
                     THSHAQAFSLHMNAANALFSRLARTCAEPRSTAPREIFARTLYESSPFARCNARRFDV
                     EVCG"
BASE COUNT          473 a          821 c          787 g          509 t
ORIGIN      
        1 cacacctgcg gacaggtgca agtccgaggc gttattcttt acggcgaaag cgaggagctg
       61 ggcgacgtcg acggccatgc ggtgtccctt gaaaagcgcc gttgagtata gacccgggta
      121 tcactgatca atatgttatc caagccgcag aattgctctg agggcgtcat acgcctccac
      181 agtcgcatcg cgcaggcctg cctggcggcc tcccgaagtg tcgattccgt cacgcttctg
      241 gccgtttcga agggtcaaag cagcgcagca atcaacggcg ccgcaagcgc cggaatcgag
      301 gatttcggcg agaacttcct gcaggaaagc ctccccaaga tcgaaactct tgccggtcgc
      361 gaactcacct ggcacttcat cgggcgcctt caggctaaca agacgcggcc ggtggccgaa
      421 aatttcgcat gggtgcacac cgtggaccgc ctgaagatcg ccgagcgcct gtcggcgcag
      481 cgcccggatc acctgccccc gctcaacctg tgcctgcaac tccacgtcgg tggtgaagcc
      541 agcaagggag gtatcgaagc tgccgacgtc gcgccgcttg cggccgcggt ccgcgaactg
      601 ccgcgcctcc gtctgcgcgg tctcatgtgc atgccgcccg ctgaagattc cattcctcgt
      661 cagcgccagt ggtttcgcga gactcgccag gtgttcgact atctcaacga acgcggcctg
      721 ggcctcgaca ccttgtcgat gggcaccagc ggggacttcg aagcggcgat cgccgagggc
      781 gccaccatgg tcaggatcgg caccgccatt ttcggcccgc gtgattccac cgccccgtaa
      841 aacaacaact atcatcacca acacatgaac atcaatgcag cgttcgtcgg cggcggcaac
      901 atgggtggcg ccctcatccg tgggctcatc gcccgcggcc tctccccaca gaacatcagt
      961 gtcggtgagg ccaaccagca gcggcgcatc gcgcttgccg atgagctcgg ggtacacgtc
     1021 accgccgaca atcgcgaagc cgtggccagc gccgacgtcg tggtactcgc agtgaagccg
     1081 caggatatgg cgggcacggt gcaggcactc gccgatgttt ttgcgcaacg gccaccactc
     1141 gtgttgtcga tcgccgcggg catccgcatc gcggacatcg tgagctggtg cgggccgggc
     1201 gtcgccgtag tgcgtgccat gcccaatcgg ccggccctga acagcgcggg cgcgactgcg
     1261 atgtatgcgc cggtttccct gagcgaggcg catcgtggcc tcgccgcggc cgttctcggt
     1321 gccgtcggta ccgcagtgtg ggtgcatgaa gaggatgcgc tcgacgtggt caccgccctg
     1381 tcgggcagcg gtcccgccta cttcttcctg cttgccgaat tgatgaccga tgccgcggtg
     1441 aacctcggcc tcgaccgcgc cagcgcgcag gagctcagca tccagacgct gttcggatcc
     1501 ggacgcatgg cgcgcgacag cgacggcgac ctggcccggc tgcgagccga ggtcacctcg
     1561 aaaggcggca ccacggaggc cgcgttgcgc tcattcgact cggcaaacct gcgtggtatc
     1621 gtcgcggccg caatttctgc cgctactgac aggagccggg aattggcaca ggcatttgga
     1681 aataagaaat aaccatgaac gccctctact acctcgtcga cacccttacc tcgctctatc
     1741 tcggcgtgct catcctcagg ctgatcatgc agattgcgcg cgccaacttc cgcaatccgt
     1801 ttgccgaagc ggtgctccgg ctcacgaatc ccttgatcat gccgctgcgg cgcgtgttgc
     1861 cgccggtcgg caagatggat tcggccacgg tgctcgccat cgtgctgttc gccatcttca
     1921 aggtcgtatt gctgtcgctg ctgttcggcg ggggcctgct ctcgctcgtc gccatggcga
     1981 tcgtggtgct gcgcacgctc gtgctcgcgg tgttgtggct gtacttcatc ctgatctttt
     2041 tctatggaat cgcgggattt ctcatgcagg gcggcagttc gccggtttac gaagtgctcg
     2101 cgtacgtctg cgatccgatc ttgaatcgca tccggaaaat cattccgtcg ctcgtggcgg
     2161 gcctggatct gtcgttcctc tgggccctca tcgcgattca ggcgctgatc atcctgatcg
     2221 gggaaatccg gctctgaacc gccgatcgac ctctttacga caacgactta gccacacacc
     2281 tcaacgtcga atcgacgcgc gttgcaacgc gcgaatggcg aagattcgta cagcgtgcgc
     2341 gcgaaaattt cacgcggtgc agttgaacgc ggttcggcac atgtgcgcgc gagcctagaa
     2401 aacaaggcat ttgccgcatt catgtgcaac gaaaatgctt gcgcgtggga atgtgtgtga
     2461 tatgttgcgc gccgaacagc tcgccgcgga tattttttcc gctgcaattt ttttacgagg
     2521 agtcgagtaa tggcaaagaa aggtggcgca ccgacgaagt cggaaatcgt ggcaacgatc
     2581 tgcaaggaca
//
LOCUS       DASQJS010000382         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_246429, whole genome shotgun sequence.
ACCESSION   DASQJS010000382 DASQJS010000000
VERSION     DASQJS010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246429"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2010
                     /locus_tag="VEW08_15055"
     CDS             <1..>2010
                     /locus_tag="VEW08_15055"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYJ42110.1"
                     /translation="EATFLNREEVQLYPVYRDPIHDFGIYRYDPKKLRFIRPRSLPLF
                     PDGAQIGREIRVIGNNAGEQLSILAGTIAKLDRDAPEYGIGKYNDFNTFYLQAASGTS
                     GGSSGSPVIDIQGRVIALNAGGATGAASSFYLPLGRVKRALDLIQAGKPVPRGALQVV
                     FHYTPYDELRRLGLNEKTEESARKAFPALTGMLVVNEVLPGGPADGTLQPGDVLVRVN
                     GKLVSEFEPLEQVTDDTVGSTVDLELERGGKPIAAKLNVGDLHAITPSAYLEFGDTVV
                     HTVSYQMARHFNVAVNGVYVANPGYIFGAAGVPRGAVISSANGKATPSLAAFEAVVAG
                     LADGERFTVRYSTVDDPNGSSVRSARMDRRWFPANHCLRDDAAGVWQCASLPNLGSAK
                     PEVGGSTRFSKPEDQRAASLAASLVGVGFDMPYSVSGITERNYRGTGLVVDATRGLVV
                     VDRNTVPVSMGDVRIVFAGTLEIPGKVEFVHPLHNLAIVSYDPKLVGNTPVRSAKLLD
                     RKITLGEALWVVGMGPDSELNTRATQVASLTPIQLPLSRTMQFRESNLETIQLVNGPT
                     DFDGALTDSDGAVVGLWSSFAWESGRELQQENRGVTIGVVSDMLRRVQAKQPLYSLEA
                     EFVPQPLAAARRLGLPDAWLRKFELASPNQRQVLSIVRLVGGSPAV"
BASE COUNT          333 a          681 c          664 g          332 t
ORIGIN      
        1 ggaagccacc ttcctcaacc gcgaggaagt gcagctctat ccggtgtatc gcgacccgat
       61 ccacgacttc ggcatctatc gctacgatcc gaagaagctg cgattcatca ggccgcgctc
      121 gctgccgctg ttcccggatg gcgcacagat cggccgcgag atccgcgtca tcggcaacaa
      181 tgccggcgaa cagctctcca tcctcgccgg aaccatcgcc aagctcgatc gcgatgcgcc
      241 ggaatacggc atcggcaagt acaacgactt caacaccttc tacctgcagg ccgcgtcggg
      301 cacgtcgggc ggttcttcgg gctcgccggt catcgacatc cagggccgcg tcattgcgct
      361 gaatgccggc ggcgcgaccg gcgccgcgtc cagcttctat ctaccgctcg gccgcgtgaa
      421 gcgcgcgctc gacctcatcc aggcgggcaa gcccgtgccg cgcggtgcgc tgcaggtggt
      481 gttccactac acgccgtacg acgagctgcg ccgcctcggg ctcaacgaga aaaccgagga
      541 gtccgcgcgc aaggcattcc cggcgctcac cggcatgctg gtggtcaacg aggtgcttcc
      601 gggcggcccg gccgacggca ccctgcagcc cggtgacgtg ctggtgcgag tgaacggcaa
      661 gctggtctcg gaattcgagc cgctcgagca ggtgaccgac gacacggtcg gcagcacggt
      721 ggacctggag ctcgagcggg gtggcaagcc catcgccgcc aagctcaacg tgggcgacct
      781 gcacgcgatc acgccatctg catacctcga gttcggcgac accgtggtgc ataccgtgtc
      841 gtaccagatg gcgcgccatt tcaacgtcgc cgtgaacggc gtgtacgtgg cgaacccggg
      901 atacatcttc ggcgcggccg gtgtaccgag gggcgcggtc atctccagcg ccaatggcaa
      961 ggcgacgccg agcctcgccg ccttcgaagc cgtggtggcc ggtctcgccg acggcgagcg
     1021 gttcaccgtg cgttattcca ccgtggacga tccgaacggc tcgagcgtgc gcagcgcgcg
     1081 catggatcgc cgctggttcc ccgccaacca ttgcctgcgc gatgacgccg cgggcgtgtg
     1141 gcagtgcgcg tcgttgccca acctcggttc ggcaaaaccc gaggtgggcg gcagtacacg
     1201 cttctcgaag cctgaggatc agcgcgccgc gtcgctcgcc gcgtcgctcg tgggcgtggg
     1261 cttcgacatg ccgtactcgg tctccggcat caccgaacga aactatcgcg gcacgggact
     1321 cgtggtggac gccacgcgcg ggctcgtcgt ggtcgatcgc aatacggttc ccgtgtcgat
     1381 gggcgacgtg cgcatcgtct tcgccggcac cctggaaatt cccggcaagg tggagttcgt
     1441 gcacccgctg cacaacctgg ccatcgtcag ctacgacccc aagctggtcg gcaacacgcc
     1501 ggtgcgcagc gccaagttgt tagaccgcaa gatcacgctg ggcgaggcgc tgtgggtggt
     1561 cggcatgggt ccggacagtg agctcaacac gcgcgccacg caggtcgctt cgctgacgcc
     1621 catccagctg ccgctgtcgc gcacgatgca gttccgcgag agcaatctcg aaaccatcca
     1681 gctcgtcaat gggcccacgg acttcgacgg cgcgctcacc gattcggacg gtgcggtggt
     1741 cggcctgtgg tcgagtttcg cctgggaaag cggccgggag ctgcagcagg aaaaccgtgg
     1801 cgtgaccatc ggtgtcgtct cggacatgct ccggcgcgtg caggcgaaac agccgctgta
     1861 ttcactcgaa gccgagttcg tgccgcagcc gctggcagcc gcacgccgtc tcggtttgcc
     1921 tgacgcgtgg ctgcgcaagt tcgaactggc gagcccgaac cagcgccagg tgctgtccat
     1981 cgtgcgcctg gtaggcggat ccccggcggt
//
LOCUS       DASQJS010000383         4421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2447656, whole genome shotgun sequence.
ACCESSION   DASQJS010000383 DASQJS010000000
VERSION     DASQJS010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2447656"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VEW08_15060"
     CDS             <1..264
                     /locus_tag="VEW08_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HYJ42111.1"
                     /translation="EAQNTTPEQMKMFLTRIGFGSRAVVTGDVTQTDLPAGKLSGLRH
                     VVNILRNTEGVAFTFFEAADVVRHPLVQRIVKAYEAHTPPDAL"
     gene            261..737
                     /gene="ybeY"
                     /locus_tag="VEW08_15065"
     CDS             261..737
                     /gene="ybeY"
                     /locus_tag="VEW08_15065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HYJ42112.1"
                     /translation="MSAARALKLELARGTRAWAPARSRMHTWAAAALGKQGEGREIAV
                     RVVAARESRELNKLWRGKDKPTNVLSFPAPVQSKRGMPRDITLPLGDLVICAQVVRQE
                     AVRDGKPVEAHWAHMIVHGSLHLAGFDHEVGLRQRLRMERREISVLKRFGIRNPYL"
     gene            734..1633
                     /locus_tag="VEW08_15070"
     CDS             734..1633
                     /locus_tag="VEW08_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HYJ42113.1"
                     /translation="MSAKNKEKDKEKERDIGASGRWLRKLSRKIGGEVQDRGEVVEYL
                     REAALREIIEGDALVMLEGVLGVADIQVRDIMVPRAQMTVLNGTDDGDTLLKTVVESG
                     HSRFPVMDEDREKIIGVLLAKDLLRLAVKDTTPFDIKEFMRPAVFVPESKRLNVLLRE
                     MRRSRVHMAIVADEYGGIAGLVTIEDVIEQIVGDIDDEHDIDEDVNIRKDGERQYVVR
                     GQTPIDEFNEYFQTELSDDEFDTVAGLVMKQLGRLPRRGETLQLADCEIKVMRFDRRR
                     IDSLRLTAPRDIVAPEYRPLVKE"
     gene            1630..3159
                     /gene="lnt"
                     /locus_tag="VEW08_15075"
     CDS             1630..3159
                     /gene="lnt"
                     /locus_tag="VEW08_15075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HYJ42114.1"
                     /translation="MNLRESLERRFVGSMVAFIAGALLALSFAPFGWWPLAILMPAVL
                     MWLWEGATPRRAAVLGFWFNAGTFSVGTYWLYIAIKQIGHAPLVLALFLMVGFVAIMG
                     AYHALLGWLVAKYLPQRGAMRWLVGIPGAWLLIEWWRSWFLSGFGWLALGYAHTDNWL
                     GGLAPVIGQFGLGLLTLLLAGALVTLLLGDRRSRIVSGGIFVVIWGAALGVRGIEWTE
                     AYSRPIEVAVVQGAIPQDEKWLTENLESILEVYRTRTREAHGADLIVWPESAIPDLAN
                     HHIEFYREVYQEASARGSSLMMGTLREEDNPKTGEEEYFNSVLSMDKSTPGVGWYDKH
                     HLVPFTEVVPVPGFVRQWLRLMSLPYSDFNRGAAQQAPLESAGQRISASVCYEDAYGA
                     TQLPALRTATLLVNVTNDAWFGRSPARYQHLQISRLRAWEAGRPMVRAANDGVSAVIG
                     ARGEIVKSAPEYEANVMRASVQPRIGLTPYARTGNWLIVCLALVFGLAAAYVGRRSKK
                     A"
     gene            3261..>4421
                     /locus_tag="VEW08_15080"
     CDS             3261..>4421
                     /locus_tag="VEW08_15080"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HYJ42115.1"
                     /translation="MSLFVARRLAGVAIVAFAVGACSPQSSVAAAAAQAEQPAPIAAP
                     AQPPSSPQPVGTLNGRVLPDFATLVEQVGPAVVNVSVLEKARRVGKQSSEDADDPVQE
                     FFRRFGMPNGPQGRGFESQPRQGEGSGFIVSADGYILTNAHVVADADEVTVRTTDRRE
                     YTAKVVGIDKRTDVAVIKIEGKDLPVVRLGDPSKLRPGEWVLAIGSPFTFENSVTAGI
                     VSATGRSMPGEDLVPFIQTDVAVNPGNSGGPLFNLNGEVVGINSQIYSRSGGYMGLSF
                     AIPIDVANNVRQQLVSTGKVTRGRIGVQIQEVNAQFADAFGLDRPRGALVGQVIDDGP
                     AAKAGIKTGDVILSVNGKNVERSAALPGVISAIKPGDTAKVEVWREGASKTLA"
BASE COUNT          763 a         1411 c         1467 g          780 t
ORIGIN      
        1 gaggcgcaga acaccacgcc cgagcagatg aagatgttcc tcacgcgcat cggcttcggc
       61 tcgcgcgcgg tggtcaccgg tgacgtgacg cagacggacc tcccggcggg caaactctcg
      121 ggcctgcggc acgtcgtcaa catcctgcgc aacaccgagg gtgtcgcgtt caccttcttc
      181 gaagcggccg acgtggtccg gcacccgctc gtgcaacgta tcgtgaaggc gtacgaagcg
      241 catacgccgc ccgacgcgct atgagtgcgg cgcgggcgct gaaactggag ctcgcacgcg
      301 gcacccgcgc ctgggcgccg gcgcgctcgc gcatgcacac ctgggccgcc gcggcgctgg
      361 gaaagcaggg cgaagggcgc gagatcgcgg tgcgtgtcgt ggcggcgcgc gagagccgcg
      421 agctgaacaa gctctggcgc ggcaaggata aacccacgaa cgttttgtcg tttcctgcgc
      481 cagttcaatc gaagcgcggc atgcccagag atataacact gccgctgggt gacctcgtca
      541 tctgcgcgca ggtcgtgcgc caggaagccg tgcgtgacgg caagccggtc gaggcgcatt
      601 gggcgcacat gatcgtgcac ggctcactgc acctggctgg cttcgaccac gaagtgggcc
      661 tccgccaacg gctgcgcatg gagcgccgcg agatttccgt gctgaaacga ttcgggatca
      721 ggaatcccta cctgtgagcg ccaagaacaa agagaaggac aaggaaaagg aacgcgacat
      781 cggcgccagc ggccgctggc tgcgcaagct gtcgcgcaag atcggcggcg aagtgcagga
      841 ccgcggcgaa gtcgtcgagt acctgcgtga agccgcgttg cgcgagatca tcgaaggcga
      901 cgcgctcgtg atgctggaag gcgtgctcgg cgtcgccgac atccaggttc gcgacatcat
      961 ggtgccgcgc gcccagatga ccgtgctcaa tggcaccgac gacggcgata cgctgctcaa
     1021 gaccgtggtg gagagcggcc attcacgttt cccggtgatg gacgaggacc gcgagaagat
     1081 catcggcgtg ctgctcgcca aggacctgct gcggctggca gtaaaggaca cgacgccgtt
     1141 cgatatcaag gaattcatgc gtcccgccgt gttcgtgccg gagtccaagc ggctcaacgt
     1201 gctgctgcgc gaaatgcgcc gttcgcgcgt gcacatggcg atcgtggccg acgaatacgg
     1261 cggcatcgcc ggcctcgtga ccatcgagga cgtgatcgag cagatcgtcg gggacatcga
     1321 tgacgagcac gacatcgacg aggacgtgaa catccgcaag gatggcgagc gccaatacgt
     1381 cgtgcgtggc cagacgccca tcgacgagtt caacgaatat ttccagaccg agctgtcgga
     1441 cgacgagttc gataccgtgg cgggcctggt catgaagcag ttaggccggc tgccgcgccg
     1501 cggcgagaca ctgcagctcg ccgactgcga gatcaaggtc atgcgcttcg atcgccggcg
     1561 tatcgattcc ctgcggctca cggcgccgcg cgacatcgtg gcgccggaat accggccgtt
     1621 agtaaaagag tgaacctgcg cgaaagcctg gaacgccggt ttgtcggcag catggtcgcg
     1681 ttcatcgccg gcgcgctgct ggcgctgtcg ttcgcgccgt ttggctggtg gccactcgcc
     1741 attctcatgc cggcggttct catgtggctg tgggagggcg caacaccacg ccgcgcggcc
     1801 gtgctcggat tctggttcaa cgctggcacg ttctccgtcg gtacgtactg gctgtacatc
     1861 gccatcaagc agatcgggca cgctccgctg gttctcgcgc tgttcctcat ggtcggcttc
     1921 gtcgccatca tgggcgccta tcacgcgctg ctgggctggc tggtggcgaa atacctgccg
     1981 caacgtggcg cgatgcgctg gctcgtgggc attcccgggg cgtggctgct catcgagtgg
     2041 tggcgcagct ggttcctgtc cggtttcggc tggctggcgc tcgggtatgc gcacaccgac
     2101 aactggttag gcggcctcgc gcccgtgatc gggcaattcg gcctggggct cctcacgctc
     2161 ctgctggccg gtgcgctggt gacgctcctg ctcggtgaca ggcgctcgcg catcgtcagc
     2221 ggcgggatct tcgtcgtcat ctggggtgcg gcgctgggcg tgcgcggcat cgagtggacg
     2281 gaagcctaca gccggccgat cgaggtcgct gtggttcagg gcgccattcc gcaggatgag
     2341 aaatggctga ctgaaaatct cgagtccatc ctcgaggtct atcggacgcg cacgcgcgaa
     2401 gcgcatggcg ccgatctcat cgtctggccc gagtccgcca tccccgacct ggctaaccac
     2461 cacatcgagt tctatcgcga ggtttaccag gaggcgagcg cgcgcggctc gtcgctgatg
     2521 atgggtacgc tgcgcgaaga ggacaatccc aagaccggtg aagaggaata cttcaactcg
     2581 gtgctgtcga tggacaagtc cactcccggc gtgggctggt acgacaagca tcatctcgtg
     2641 cctttcacgg aagtcgtgcc ggtgcccggg ttcgtgcgcc agtggctgcg gctcatgagc
     2701 ctgccttact cggatttcaa tcgtggcgcg gcgcagcagg cgccactcga atcggcgggg
     2761 caacggatct ccgccagtgt ctgctacgaa gacgcctacg gggcgacgca actgcccgca
     2821 ttgcgcaccg ccacgttgct cgtgaacgtc accaacgacg cctggttcgg gcgttccccc
     2881 gcgcgctacc agcatctgca gatatcgcgc ctgcgtgcct gggaggccgg gcgccccatg
     2941 gtgcgcgccg ccaacgacgg cgtttccgcg gtgatcggtg cgcgtggcga aattgtcaaa
     3001 agtgcgccgg aatatgaagc gaatgtaatg cgtgccagcg tccagccacg tataggtctt
     3061 acaccctatg cgcgtaccgg taactggctg atagtctgtt tggcccttgt tttcggcctt
     3121 gcggccgcat atgtggggcg tcgtagcaag aaggcgtgaa ggagtggtcc atggatccgc
     3181 agacgctgaa cttaggcatt tccagccggt ctatttcgtc aatgtcgttt tccccgtcac
     3241 agctccgagg gtttgctgat atgtcgctat tcgttgcaag gcgccttgct ggtgtcgcga
     3301 tcgtggcctt cgcggtcggc gcatgctctc ctcagtcctc cgttgccgcc gccgcggcgc
     3361 aggccgaaca gccggccccc atcgccgcgc ccgcgcagcc tccatcttcg ccgcagcctg
     3421 ttggcacgct caacggccgt gttctcccgg atttcgccac gctcgtagag caagtgggtc
     3481 ccgccgtcgt caacgtctcc gtgttggaga aagcccgtcg agtgggcaag cagtcttccg
     3541 aagatgcgga cgatcccgtg caggaattct tccgtcgctt cggcatgccg aacggcccgc
     3601 agggccgtgg cttcgaatct cagcctcgcc agggtgaagg ctcgggcttc atcgtcagcg
     3661 ccgacggcta catcctcacc aacgcgcacg tggtcgccga cgccgacgaa gtcaccgtgc
     3721 gcacgacgga ccgccgcgaa tacaccgcca aggtcgttgg catcgacaag cgcaccgacg
     3781 tcgccgtcat caagatcgaa ggcaaggacc ttcccgtcgt gcgcctcggc gacccgtcga
     3841 agctgcgccc cggtgaatgg gtgctcgcca tcggttcgcc gttcacgttc gagaacagcg
     3901 tcaccgcggg catcgtcagc gccacgggtc gctccatgcc gggtgaagac ctggtgccgt
     3961 tcatccagac ggacgtcgcc gtgaatcccg gcaactccgg tggcccgctg ttcaatctca
     4021 atggcgaagt ggtgggcatc aattcgcaga tctacagccg cagtggcggt tacatgggcc
     4081 tgtcgttcgc catcccgatc gacgtggcca acaacgtgcg ccagcagctg gtgagcaccg
     4141 gcaaggtcac gcgcggccgc atcggcgtgc agatccagga agtgaacgcg cagttcgcgg
     4201 atgccttcgg cctcgaccgc ccgcgcggcg cgctcgtggg ccaggtcatc gacgatggtc
     4261 cggccgcgaa ggccggcatc aagaccggcg acgtgatcct cagcgtcaac ggcaagaacg
     4321 tggagcgttc agcggcgctg cccggggtca tctccgccat caagccgggg gacaccgcca
     4381 aggtcgaagt gtggcgcgag ggcgccagca agacgctcgc c
//
LOCUS       DASQJS010000384         6713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4019608, whole genome shotgun sequence.
ACCESSION   DASQJS010000384 DASQJS010000000
VERSION     DASQJS010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4019608"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1289)
                     /locus_tag="VEW08_15085"
     CDS             complement(255..1289)
                     /locus_tag="VEW08_15085"
                     /inference="COORDINATES: protein motif:HMM:NF008106.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYJ42116.1"
                     /translation="MVKSRIRAAALVVCASVVLGSCGNGPSSDVTLLNASYDPTRELY
                     EDINAAFAKHWKQTTGQDVRIDQSHGGSGRQARAVIDGLPADVVTLALAGDIDKIAAE
                     GKLLPLNWQQRLPHNSAPYASTIVFLVRAGNPKNIRDWGDLVRADVQVITPNPKTSGG
                     ARWNYLGALAWAEKQPGASEASAREFVRKLYKNVPVLDTGARGSLTTFAQRGIGDVAI
                     SWENEAHLAMRERGSALVEIVVPSISVLAEPSVAVVDKVVLRRGTRSVAQAYLEFLYS
                     PEGQEIAARNFYRPRDATVAAKYASRFAQVDLVTIEHFGGWKVVQKEHFANGGIFDQI
                     TSASDGDAAR"
     gene            complement(1321..2808)
                     /locus_tag="VEW08_15090"
     CDS             complement(1321..2808)
                     /locus_tag="VEW08_15090"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HYJ42117.1"
                     /translation="MQARNHGAVSAAISTVPETNTARRAWHIAIVGAGFSGTALAINL
                     LKEAAATASPRHMRISLVDPRPEIGGGVAYATRDYPYPLNVAAGQMSVDGGRPGDFLD
                     YLKAQGIHASPGDYLPRQVYGDYLRARFAEARAAAPKTVECVHHRASAWQLRRNDDRW
                     LLWLDDGTPLPADDVALALGNPPPATPAELAHLLSSARYVSDPWSIGSLANQENIGSV
                     LLVGSGLTMVDAALRLAALRPRVRHIHVLSRHGWMPEPQASASLPAIKPDVAGALEAA
                     RGSTRRLVRAFRTLTESVDNAGGDWREVLALARGQLADQWHALDHAQRARFLRHARSA
                     WDVNRHRLPAGPLSAVRSLARAGILDVHAGRLEEVKELDDAVEVIWRPRGAARTRAWL
                     VDRVINCTGPECRAGRVADPLVQSLLSSGTIRSDALSLGIDIADDGRPVSRDGTPVDR
                     LHYLGPWLRARDWEATAVPELREHAAQLARSLARKNLVESVEQSC"
     gene            2969..3964
                     /locus_tag="VEW08_15095"
     CDS             2969..3964
                     /locus_tag="VEW08_15095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HYJ42118.1"
                     /translation="MRSILSFASLALALNLAAQAAPEKMQAVEVGQGGLSVQTRPVPR
                     PGAGEVLIKVRAAGVNPVDWKTALRRLGMVPGKDVSGTIDRLGEGVSGWKVGDPVLGF
                     AHGSGSYAEYAVISVTDLSRKPKSMTFEQAAGLPIAAETAYRALHEAGHIERGQTVLI
                     HGAAGGVGSSAVQIAKAAGARVIGTASPNNHEFLRSLGVDQVIDYKSQKFEDVVKNVD
                     LALNTVDADTTARSIVVVRPGGALVSIAGPLPNPQACAAAKIRCLRPNRETGATTAEI
                     LERVVELADSGKLKVFVEATYSLADAGKAWDKNREGHTRGKLIIVVSEGPTMKHQ"
     gene            complement(3924..4088)
                     /locus_tag="VEW08_15100"
     CDS             complement(3924..4088)
                     /locus_tag="VEW08_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42119.1"
                     /translation="MNSRNREENRADLGRVLLALAVLALLSSLVDSPASQASGTATIG
                     ASSSGLPTRR"
     gene            4225..4752
                     /locus_tag="VEW08_15105"
     CDS             4225..4752
                     /locus_tag="VEW08_15105"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYJ42120.1"
                     /translation="MSKVGTRFHFRNRVARRAQPRTRIVQALAVALMWAATQWATAQG
                     APRPESLIKWRQSAYQVIAWNSGRIKAALAGQFDSHEVQNAANALAAVANSGLAGLFA
                     PGTAHGKGWRETTARDEVFSDAGKFRALTEEFAREASALARVAAAGDRNTVNAQFLKV
                     AQTCKSCHNQFRQAD"
     gene            4798..5301
                     /locus_tag="VEW08_15110"
     CDS             4798..5301
                     /locus_tag="VEW08_15110"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYJ42121.1"
                     /translation="MNCFKSFCTAAAIGALALTGSAALLAQTPPAPGAAPAGPTPDER
                     AIEYRQAVYKIVAGNFGPLSQVAQGKADFKAENAGKQATRLAQIATFVGDAYPDISKD
                     GKTRALPAIWSNRKEFDQIVADFATHTKTLAEVVENSSSAGEELKAAVAAVGNDCKAC
                     HDKFRSK"
     gene            5330..5803
                     /locus_tag="VEW08_15115"
     CDS             5330..5803
                     /locus_tag="VEW08_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42122.1"
                     /translation="MFKKLFILAAATSAMSAFAAEPAAKPATPPKPLSHELTRAEFEQ
                     LLTRPNELLIIDLRRPDELTRIGGFPVYLSIQAKHLEQQLAWIPKDRTIVTVSNHAAR
                     SGRAADLLTAKGFKVAGLLGAQTYEEQGGKLTKIEVPPPRTPTAAAVAPAAQAGR"
     gene            5811..6509
                     /locus_tag="VEW08_15120"
     CDS             5811..6509
                     /locus_tag="VEW08_15120"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HYJ42123.1"
                     /translation="MSEIPRRRVWDLPVRLVHWLLVIAIAGSYATHKLGVAYFKYHLW
                     FGYLVVVLAAFRILWGFFGTRHARFAKFLRGPGTTWNYLRALASGVAQPTPGHNPLGA
                     WMVLFLLLSLLAQGITGLFANDEIFNTGPLYGYISDSLSLQLTSWHRRLFDWIFTAVV
                     LHVLAVFAHRLLVGHDLVGPMISGRKPASLVAEHEAIGSSRLWLAVVLLAGVAGAMSW
                     LVVQAPAPAVFSFE"
BASE COUNT         1174 a         2182 c         2212 g         1145 t
ORIGIN      
        1 ggcctcgtta ggcacgaagc cgcctctccg cttcatcagg actctagctt ccgctcctca
       61 caaccgcacg aaagtgtcat tgcgtgtggc ggcggccagc aaactagcga cgccaccccg
      121 aacgaagggc ccggcgaaaa gcgaacgaaa tcctgtgcgc ggactgctcg ctggggcacg
      181 cgcgcccccc aaaccgcccc tcgttacgaa gggcgaacga actcctgcgc gccgactgct
      241 cgccggggcc aacctcatcg agcggcgtcc ccatccgacg ccgacgtgat ctgatcgaat
      301 attccaccgt tggcgaaatg ttctttctgc acgaccttcc atccgccgaa gtgctcgatg
      361 gtgaccagat cgacctgcgc aaaccggctg gcatatttcg cggcgaccgt ggcgtctcgc
      421 gggcggtaga aatttcgtgc ggcgatctcc tgaccctccg gtgaatacag gaactcgagg
      481 tacgcctgcg cgacactgcg cgtgccccgg cggagcacga ccttgtcgac gaccgccacc
      541 gacggttccg cgagcacgct gatcgacggc acgacgatct cgacgagcgc gctgccgcgt
      601 tcgcgcatcg cgaggtgggc ctcgttctcc cacgagatgg cgacgtcgcc aatgccgcgc
      661 tgcgcgaagg tggtcaacga gccgcgcgcg ccggtatcga gcaccggcac gttcttgtac
      721 agcttgcgca cgaactcgcg cgcactggcc tcgctggcgc cgggctgctt ctcggcccac
      781 gccagggctc cgagataatt ccagcgtgcg ccgccggagg tcttgggatt gggcgtgatc
      841 acctgcacgt cagcacgcac gagatcgccc cagtcacgaa tgtttttcgg gttgccggcg
      901 cgcacgagaa acacgatggt ggaggcgtac ggtgcgctgt tgtgcggcag gcgctgttgc
      961 cagttgaggg gcagcaattt gccctcggcg gcgatcttgt cgatgtcgcc cgcgagcgcc
     1021 agcgtcacca cgtccgcggg caacccgtcg atgacagcgc gcgcctggcg gccggacccg
     1081 ccatgcgact ggtcgatgcg cacatcctgg ccggtggtct gcttccaatg cttcgcgaag
     1141 gccgcgttga tgtcttcgta gagctcgcgc gtcggatcgt aggacgcatt gagcagcgtg
     1201 acgtcgcttg acgggccgtt tccgcacgag cccagcacca cggaagcgca cacgacgagc
     1261 gcagcggcgc gaattctgct tttgaccatg gccgggaccc ccaatgagcg ggaccggaga
     1321 ctagcagctc tgctccaccg attcgaccag gtttttccgc gccaatgatc gggcgagctg
     1381 cgccgcgtgt tcgcgcagct ccgggacggc ggtcgcctcc cagtcccgcg cgcgcagcca
     1441 cgggccgagg taatgaagcc tatctaccgg cgtgccgtcg cgtgaaaccg gccggccgtc
     1501 atccgcgatg tcgatgccca gcgacagcgc gtcggaccgg atggttccgc tcgacagcag
     1561 cgactgcacc agcggatccg cgactcgccc ggcccggcac tcggggccgg tgcaattgat
     1621 gacccgatcc accagccagg cccgcgtgcg cgcggcgccg cgtggccgcc agatgacttc
     1681 gacggcatcg tcgagctcct tcacttcctc gagacggccc gcgtgtacgt ccagtatgcc
     1741 cgcgcgcgcc agcgatcgca ccgcgctcaa ggggccggcc ggcaggcggt ggcgattcac
     1801 atcccaggcg gagcgggcgt ggcgcaggaa ccgcgcgcgc tgcgcgtgat cgagcgcgtg
     1861 ccactgatcc gccagctgtc cgcgcgcgag tgccagcacc tcgcgccagt ctccaccggc
     1921 gttgtcgacc gactccgtca gcgtgcgaaa ggcccgcacg aggcgtcgag tggagccgcg
     1981 agccgcctcg agcgcaccgg caacgtcggg tttgatcgcg ggcaacgacg ccgaagcctg
     2041 tggttccggc atccatccat gacgcgacag cacatggatg tgccggacgc gcggacgcaa
     2101 tgcggccagg cgcagtgccg cgtcaaccat cgtgagaccg ctgcccacga gcagcacgct
     2161 gccgatgttt tcctgattcg cgagcgagcc gatgctccag ggatcgctga catatcgagc
     2221 gctggacaac aggtgagcga gttcggcagg tgttgccggc ggtggattgc cgagcgccag
     2281 cgccacgtcg tcggcaggca gaggtgtgcc atcgtcgagc cacagcaacc agcgatcatc
     2341 attgcgccgg agttgccatg cgctggcccg gtggtgcacg cattcgacgg ttttcggcgc
     2401 cgctgcgcgt gcttcggcga aacgggcgcg tagatagtcc ccgtacacct gcctcggcag
     2461 gtagtcgcct ggggatgcgt gaattccctg ggccttcagg tagtcgagga agtcgccggg
     2521 ccggccgcca tccaccgaca tctgtccggc ggccacattg agcggatacg ggtaatcgcg
     2581 cgtggcgtag gcaacgccac cgccgatttc cggacgcgga tcgacgagcg agatgcgcat
     2641 gtgccggggg gacgccgtcg cagcggcttc cttcaggaga tttatcgcga gcgcagtccc
     2701 ggagaatccc gcgccgacga tggcgatgtg ccatgcgcgc ctcgcggtat tcgtctcggg
     2761 tacggtcgaa attgcggcgc tgaccgctcc gtggtttctg gcctgcatgc gccgaagtat
     2821 ccggaggcgg cggtcacagc gtcgattccg taccggtttg aaatgggtta tgcgcggatg
     2881 tgacaggatc gtgcactttt caccggttga ccggtctcat gtcagtcgcc cggttttcga
     2941 cgcctgcaca tatatgagga gtgtctcaat gcgttccatc ctgagtttcg cgagtctcgc
     3001 actggcgctc aacctggctg cccaggccgc accggaaaaa atgcaggcgg tggaagtcgg
     3061 acagggcggg ctgtcggtcc agacgcggcc agtgccgcgg ccgggggccg gcgaagtcct
     3121 aatcaaggtg cgtgcagccg gcgtgaatcc cgtggactgg aagacagcgc tgcggcgcct
     3181 cggcatggtg ccgggcaagg atgtctccgg aaccatcgac aggctgggcg aaggagtctc
     3241 aggctggaag gtcggcgatc cggtgctcgg cttcgcgcat ggaagcggca gctacgcgga
     3301 gtacgccgtc atctccgtca cggacctgtc gcgcaagccg aagtccatga ccttcgagca
     3361 ggccgcgggc ctgcccatcg cggccgagac cgcgtatcgc gcgctgcacg aagccggtca
     3421 catcgagcgc ggccagaccg tgctcattca cggcgcggcg ggtggcgtgg gttcctctgc
     3481 agtgcagatc gccaaggcag ccggagcgcg tgtcatcggt accgcctcgc ctaacaatca
     3541 cgaattcctg cgctcgctgg gtgtcgatca ggtcatcgat tacaagagcc agaagttcga
     3601 agacgtggtg aagaacgtcg acctggcact caacaccgtc gacgccgaca ccaccgcgcg
     3661 ttccatcgtt gtggtgcgtc cgggtggcgc gctcgtgtcc atcgcagggc cgctgccaaa
     3721 tccgcaagct tgtgcggctg cgaagatccg ctgcctgcgt ccgaatcggg agaccggcgc
     3781 cacgactgcc gagattctgg agcgcgtggt ggagttggcc gattccggca agctgaaggt
     3841 gttcgtggag gcgacctatt cgctcgccga tgcgggcaag gcgtgggaca agaaccgcga
     3901 aggccacacg cgcggcaaac tcatcatcgt cgtgtcggaa ggcccgacga tgaagcacca
     3961 atagtcgccg tgcccgaggc ctgcgaagca ggcgagtcga ccagcgacga cagcagcgcc
     4021 agcaccgcga gtgccagcaa gacgcgcccc aggtcggcgc gattctcctc tcgattgcgc
     4081 gagttcatga cctcaccgta gccgtgcccg cgtccgcacg gaaatcgcgg gatcgcattc
     4141 gcttatgccg atcgaatcgt tccgtcgcgc cagcgcgcat ggtgaggtgc gcctctccag
     4201 ccaatctagt taggcgacga gcagatgtcg aaggtcggga cgcggtttca tttccgtaac
     4261 cgcgtggcca ggcgcgcgca accgcgcacg cggatcgtgc aggcgctggc tgtcgcgctg
     4321 atgtgggcgg cgactcaatg ggcaacggcg caaggcgccc cgcgcccgga gtcgctgatc
     4381 aagtggcgcc agtctgccta ccaggtaatt gcctggaatt ccggccgcat caaggctgcg
     4441 cttgccggcc agtttgacag ccacgaggtt cagaatgcgg cgaacgcgct cgcggcggtg
     4501 gcgaattcgg gtctcgcagg actgttcgcg ccgggcacgg cgcacggcaa gggatggcgc
     4561 gaaaccaccg cgcgcgacga agtattcagt gacgccggca agtttcgcgc gctgaccgaa
     4621 gaatttgcgc gcgaagcaag cgcgctggcc cgcgtcgccg cagcgggtga ccgcaacacg
     4681 gtcaacgcgc aattcctgaa agtcgcgcag acctgcaagt cctgccacaa ccagtttcga
     4741 caggccgatt gattcagcca cgccgcgagc ggcgctcaac ccaagggaga tgcattgatg
     4801 aactgcttca agagtttttg cacggccgcc gcgatcggcg cgctggcgct gacgggcagc
     4861 gcggcgctgt tggcacagac gcctccagca ccgggagccg cgcccgcggg gcccacgccc
     4921 gacgagcgcg ccatcgagta tcggcaggcg gtttacaaga tcgtcgccgg caattttggc
     4981 ccgctgtcac aggttgcgca gggtaaggcg gatttcaaag ccgagaacgc gggcaaacag
     5041 gcgacgcgtc tcgcgcagat cgccaccttc gtcggtgatg cgtatccgga catctcgaag
     5101 gacggcaaga cgcgcgcact gcccgcgatc tggagcaatc gcaaggagtt cgaccagatc
     5161 gtggccgatt tcgccacgca caccaagacg ctggccgaag tggtggagaa cagcagcagc
     5221 gcaggcgagg aactcaaggc tgccgtggcg gccgtgggca acgactgcaa ggcctgccac
     5281 gacaagttcc gttcgaagta acgcccaaga gaattttcag gagtcaacga tgttcaagaa
     5341 attattcatc ctggccgccg cgaccagcgc gatgtccgcg ttcgctgccg agccggccgc
     5401 gaaaccggcg acgccgccca agccgctttc gcacgaactc acgcgagccg agttcgaaca
     5461 gttgctcacc aggccgaacg aattgctcat catcgacctg cgccggcccg atgagctcac
     5521 gcgcatcggc ggtttccccg tgtacctgag tattcaggcc aagcatctcg agcagcagct
     5581 cgcgtggatt ccgaaggacc ggaccatcgt gacggtatcg aaccacgccg cgcgcagcgg
     5641 tcgcgccgcc gatctgttga ccgcgaaggg attcaaggtg gctggtctgc tcggagccca
     5701 gacctatgaa gagcagggcg gcaagctgac aaagatcgaa gtcccgccgc cgcgcacgcc
     5761 cacggccgcc gctgtggctc ccgcggcgca ggctggcagg taattcccga atgagcgaga
     5821 tcccgcggcg gcgcgtgtgg gatctgccgg tgcgcctggt gcactggttg ctggtgatcg
     5881 ccatcgccgg ttcgtacgcc actcacaagc tgggagtggc gtatttcaaa tatcacctgt
     5941 ggttcggtta cctcgtggtg gtgctcgcgg cgtttcgcat cctctggggg ttcttcggta
     6001 cgcgtcacgc gaggttcgcg aagttcctgc gcgggcctgg cacgacatgg aactacctgc
     6061 gtgcgttggc gagcggggtc gcgcagccga cgccaggcca caatccgctg ggcgcgtgga
     6121 tggtgttgtt cctgctgctc tcgttgctgg cgcaaggcat cacaggtctc ttcgccaacg
     6181 acgagatatt caacacggga ccgctgtatg gctacatcag cgacagcctg agtctgcagc
     6241 tcacctcctg gcatcgccgg ctgttcgact ggattttcac cgcggtcgta ttgcacgtgc
     6301 ttgcggtgtt cgcccaccgc ctgctcgttg gacacgacct ggtcggtccc atgatctcgg
     6361 gccggaagcc cgccagtctc gtcgccgagc acgaagccat cggatcgtcg cgcctgtggc
     6421 tggccgtcgt tctgctcgcc ggtgtcgccg gcgcgatgag ctggcttgtg gtgcaagcgc
     6481 cggcgccggc ggtattcagc ttcgaatagc gcggttacag gtttgatacc ggcactgtag
     6541 ttagacaggt tcatgcccgg ccgggcatat gtcttcactc aatcgtgatt ggaaccccgc
     6601 ggagtagcaa accagtattc ggtcgcactc cacccggaat ggatccaatg aagacgacga
     6661 acctggtctc cgcgatgttc ctgtcagctc ttggtttcat ggcagccgtc agc
//
LOCUS       DASQJS010000385        14360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3700415, whole genome shotgun sequence.
ACCESSION   DASQJS010000385 DASQJS010000000
VERSION     DASQJS010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14360
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3700415"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VEW08_15125"
     CDS             <1..575
                     /locus_tag="VEW08_15125"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HYJ42124.1"
                     /translation="ALVLQLDPATIHMSFIGAALAVSYPFFKRFFPLPQFYLGVAFSW
                     GVPMAFMAQLGEIPRVAWIIFFAGIIWAAIYDTMYAMVDRDDDLKLGLKSSAIVFGDM
                     DTLIIGVLQALMLYALYLVGTSMEYGYWYNAGLVGAGVFFAYQQWRIRERKRQSCFDA
                     FLNNHYVGYSVFIGILLEYAFRVPEQALIQ"
     gene            complement(580..2142)
                     /locus_tag="VEW08_15130"
     CDS             complement(580..2142)
                     /locus_tag="VEW08_15130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/sugar symporter"
                     /protein_id="HYJ42125.1"
                     /translation="MNLATLDIVILAIYAVGIFVLAQWVSREKGTHQKDAQDYFLASR
                     ALPWWAIGTSLIAANISAEQIVGMSGSGYVMGMAIASYEWMAALTLIVVGKWILPVFL
                     KNGIYTMPEFLERRYSPAVRNVMAVFWLALYIFVNLTSILWLGALAVNAIAGLDIQVA
                     ITALAVFALLYALYGGLKAVALTDIVQVSLLVLGGIIITYMSLDKVSGGAGVLEGFRI
                     LTTQHPEKFDMILSPDSPHYKDLPGLAVLLGGMWMMNVSYWGFNQYIIQRGLAAKSVN
                     EAQKGIVLAAFLKLLMPAIIVLPGIAALVLVPGLEKTDQAYPSLMAMLPIGIKGLVFA
                     ALVAAIVASTGSKINSIATIFTMDLYRAARPNTEQKKLVLVGRIVAVVSVVIAVFITK
                     PFLSNFGQGFQYIQNFTGFVTPGICVIFLLGLFWARTTATAAMVSMLATVVISWVFYK
                     YLPDYPFMNRVGWCFVAGIVIAVVISLMSPNKAAAMRVDINNVDFRTGTFFNVAAGLI
                     VVILAALYYTWW"
     gene            complement(2179..2787)
                     /locus_tag="VEW08_15135"
     CDS             complement(2179..2787)
                     /locus_tag="VEW08_15135"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF006600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HYJ42126.1"
                     /translation="MIEMVAILRGLTPVRAPEVGAALVSCGFRTIEVPLNSPDPFTTI
                     QLLTQAHGATCLIGAGTVLTRAEVDRVHAAGGRLVVAPNCDGEVIRHALGLGLRVLPG
                     IATATEAFTAVRHGATELKLFPASSYGAGHLKALKSVLPKHVKVFPVGGIGSQDIATW
                     LASGADGFGFGGELFKPAYTLAELTKRAQDLVQAMRDAMEKK"
     gene            complement(2784..3728)
                     /locus_tag="VEW08_15140"
     CDS             complement(2784..3728)
                     /locus_tag="VEW08_15140"
                     /inference="COORDINATES: protein motif:HMM:NF016894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HYJ42127.1"
                     /translation="MSQVSKDLAAFIAGDWGTSNLRLTLCDAEGGTLDVRKGPGAAEA
                     RGKHAEIFDTLSADWRRAHGELPAVLSGMVGSTIGWLEVPYLPCPAELDELAEAPARV
                     RDGVFIVSGMRCSNPLGAPDVMRGEETQLLGARYLDASLEVGAQLVCMPGTHTKWVSL
                     HKGVVQEFLTAPTGELFAMLCEHSVLVRDKATPIVHQPVDFERGLAESAHHPQVNLLH
                     RLFQGRSLRIDKQLSAEGAASWMSGLLIGSDVAGALSLFPDLPRAASVYVIGTPQLND
                     SYSRALARHGRKTVCIEGDKAALAGLTFVHLELERQKS"
     gene            complement(3725..4492)
                     /locus_tag="VEW08_15145"
     CDS             complement(3725..4492)
                     /locus_tag="VEW08_15145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ42128.1"
                     /translation="MTKIEDLNGKAVLITGASSGIGAALARAFAAQGAKLALHYNAHE
                     EAARALAAEIAPGSPGASLIRGDLSKRGEAKRVVNEAAAALGGLDVLINNAGGLGERR
                     PIADVDDALFDFVYDLNVRSVIAASQAAIPHFEKRGGGNIVNVGSIAGIDGGGPGSSM
                     YATAKAAVHNITRHLATDLAAKNIRVNTVAPGAIATPFHARTPQARMDAMRDATAMGR
                     VGVAEDCAGAFLFLASNELSGYITGNIVHVNGGMYMP"
     gene            complement(4503..6575)
                     /locus_tag="VEW08_15150"
     CDS             complement(4503..6575)
                     /locus_tag="VEW08_15150"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HYJ42129.1"
                     /translation="MATARWLWALLALQLCAAAGAATRIDLTRDWAFRTEKGVEAGAT
                     LASWPKAIPAGTRSVTVPHTWNREGADYDYLGTGWYFRRFDLPKLPADAIAQLHFGAT
                     FYKARVWLNGVEIGSHEGGYSAYSFDVTSQLRDDNLLVVAIDNRPGMFTIPGFGARGA
                     PDAWYDWWAYGGIVRDVWLSVHGPVRIDKQFIRSKLDGVNAAVTDRVTLVSRSAQRLS
                     LHATVTGPNGETAGEHTQPVTLKAGANEVSFAIGVKQINRWDLDHPNLYRMTVALIDS
                     SGKAIELANDSFGLRELVIRDRHLLLNGQRVRLTGMTRHTDSPWEGLAESPGTLRHDW
                     EDMKSLNMTLTRPVHYPPGTRAADFADRRGILLIPEIPIWQASEEQLSNPQYLELAKQ
                     QMRELIEQYGNHPSVFAWSVLNESAAGSPGGIRFFRAMRDYIKSLDPERPVTLADDNL
                     PKLKTAAESAANDADFLMMNQYFGAWHGPREALDPALDKVDRLFPGKMVIISELGFPG
                     IFAKNATEADATRVSIIREQLPLLAKRDWIAGAILWCYQDYKSRRYFWPGQEQGYLEH
                     GIVDENRQRKPSYFAWAQVNAPAHLDTRWTKNADGKPTAFAFTLKPNGEQEMPRYPMN
                     GYRVSWQLVEADNKEFANGGALLNPPGALSVERDVPARAQQGAFKLVIKLLRPDGSLA
                     MERALSSQ"
     gene            complement(6579..7508)
                     /locus_tag="VEW08_15155"
     CDS             complement(6579..7508)
                     /locus_tag="VEW08_15155"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYJ42130.1"
                     /translation="MRLKVGLLGIGKIARDQHIPSLRASPRFTLAACASRNATVDGVS
                     NFPDLEAMLAAVPDLQCISICTPPQAHFDAALTSLRAGKHVMLEKPPAATTRQIALLA
                     DEAARCGRTLFQTWHSRFAAGVDAAREFLRSRKLVSGKINWKEDVYQWHPGQRWIWEP
                     GGFGVFDPGINALSLLTEILPTDVCVEAALLEFPENQQSPIAANVRMRTEDGVEIGAE
                     FDFRHKGEQSWDIDLMTTGGKLKLRGGGAELYIDGKAITRDASMAGEYPRLYERFASL
                     CEAGKSEVDWRPFQLVADAFLIGERRIVAPHEI"
     gene            complement(7542..8672)
                     /locus_tag="VEW08_15160"
     CDS             complement(7542..8672)
                     /locus_tag="VEW08_15160"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HYJ42131.1"
                     /translation="MNSRLRERGVVIVSMLITALAATAADTSPKRFAGVDLSYVNEVE
                     SCGAQFRLDGKLRDPYELFAESGANLVRLRLWNNPTWTKYSTEADVTRSIERARKAGM
                     QVLLDFHYSDDWADPQKQTIPAAWAADIGDTDKLATHVYEYTRDVLERLNTRGLLPYM
                     VQVGNEINTQMLRPAETKGEPIDWARNAKVLNAGIRAVREAKDANGTSPKVMLHVAQP
                     ENVEPWFAAATAAGVRDFDYIGVSYYPKWSTFNMKQAGAALATVRQRFKADIIVVEVS
                     YPWTLKDAGDKAANLLGEDSLLRGYPATPGGQQHFMNDVVRMTREAGGVGVVYWEPAW
                     VSTSCATRWGTGSHWENATLFDFRKGNELTPAADFLKAILAE"
     gene            complement(8755..11379)
                     /locus_tag="VEW08_15165"
     CDS             complement(8755..11379)
                     /locus_tag="VEW08_15165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ42132.1"
                     /translation="MHSKAHRRALASMAALITAPVAMSQEAPAQKAEAEEIGEIVVSG
                     YRASLQSALEVKRSSELVVDAISGGDIGALPDVTIAESLVRLPGVNGTRDRGNQSQAT
                     LRGLGPRLVLGLVNGREVASSEPNRNVRWEIYPSEVVSGADVYKSQSADIVAGGVAGT
                     IDIKTIQPLNYTGPEILLRGGPVYYEAGADIPGYDPYGYRGSASFTHSVGDNFAFNLG
                     ASAQRQKNAFPSFQGWGYNDESMHSTDATGDIDGDGEIDPTPWGAQTEVKKLTEDRYG
                     VNAAIGWRPTDSFELNVDALYSKFTIDEDQNQAWYGRNFWGNWDNGQAWCYDGAQSSY
                     TVENGSVVGATLDNCFAPVTNVIAKYTEDKDLLVTGVNGKFETESWTVSGDLSYSDAQ
                     RTNRWAAFMSEVWPATMTYDMSAGVRPSVTTSSDPSDPMIQAAPNWLPGQSDGPDDLT
                     DELSAVRFDATRRFEGAVKALQFGVRFSEREKDYTRREADFIGTAAGPLPASLFSSFS
                     VKEFDAPPLLNGDFDEIVDYVYGGMPASASAVVPTSGWNVEESVSEAYAMLRFGGTMG
                     SPFTGNAGVRFVRTETTSSGFQSFNGGAAQPLSYDNDYTEALPSLNLTFNLTDSNLLR
                     FGAARVIARPPLDELRASRTLWNQAQPFTGQAGNALLDPFLATQFDVSYEFYFGPEAL
                     FAMAAFYKDVETHIGYTTVPEVIGGDTYQVTQPNNGDGGDIAGMELTFQTPFAREGAL
                     RNFGIYTNYAFVDTDVSEFYPVTDPLPIEGYAKHTAVIDLWFQKSGIEARLGWKYHSP
                     FSIIAGWNGSDVRSLGTERILDFSTSWQVNEMFGMRLQLSNLTDEPLRITRDNNTSRL
                     GSFDVYGRRALLDFTFKF"
     gene            complement(11573..12274)
                     /locus_tag="VEW08_15170"
     CDS             complement(11573..12274)
                     /locus_tag="VEW08_15170"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HYJ42133.1"
                     /translation="MSGTINLTQSLVQKLGSSIVRGELPAGKPLPIEAELGKKFGASR
                     TVMREAVKILSTKGLIGQRPRVGTYVHPEEKWDLLDAEVLKWILDRHFSHRLVREFLE
                     VRIGIEPAAAALAATNATDEDKELLKQKLEKMKQANDGHFDPVSADIAFHATILEISH
                     NRFFHQLTPIIETALRFSIRLTNKVKGTLPDYDAHEKIYRAIRNGNSEAAFRACRELI
                     KEALMLVIKTDKART"
     gene            complement(12438..13187)
                     /locus_tag="VEW08_15175"
     CDS             complement(12438..13187)
                     /locus_tag="VEW08_15175"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ42134.1"
                     /translation="MPTKIHVNDVRKSFQNDKVRLQVIEGLDFKVNDGEFVAIVGPSG
                     CGKTTLMNIIAGFLAPDSGTVNVNGVVRNGPNRKGIMITQQGSVFPWLTVKQNLLFGL
                     PDDVPNRETLADEYAALVGLKGFENNYPHELSGGMLKRAEIARALVVKPEILYMDEPF
                     SALDALMSLRMQNELLRILAIEKHTVMLITHDVEEAIHLADRILVLSPRPARIQASFD
                     VPFAHPRRVSSPEAQALRVAILRELGVTEAV"
     gene            complement(13177..13956)
                     /locus_tag="VEW08_15180"
     CDS             complement(13177..13956)
                     /locus_tag="VEW08_15180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ42135.1"
                     /translation="MVGEQKQMKGLKQALPSIVVIAVVVGIWWAIVIATESAIFPTPW
                     AVVAGTLELARDGTLWEHIGASLMRVGAGFGLAVALAIPLGLWMGWVHGAFVTLNPLF
                     QILRPISPIAWIPIAILWFGVGNASPIFLIFIASVFPMIVQTTVGVHTIEKRFLRAAE
                     NFGVSRSKLFLKVVFPATLPQIIVGMRIGLGVAWLVVVAAEMIALRSGLGYLIIDSRN
                     AGNRYDLVIAGMIIIGLIGLMLDGFMRLLESMKVVRWRYAH"
     gene            complement(13932..>14360)
                     /locus_tag="VEW08_15185"
     CDS             complement(13932..>14360)
                     /locus_tag="VEW08_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42136.1"
                     /translation="SSIRRAWIAVPLLAVLAVLGWAGFAPVSVPSRDELFEIPKGTYA
                     RRMAGNKVEILPSKIRLTLGLNDVLMLRNLDDVPQVFGPTIMMPGQSFRLPFEKAASY
                     DFACTAHASGQMTVLVEPYPAMPWSKVRWRVREWWENKSR"
BASE COUNT         2610 a         4539 c         4496 g         2715 t
ORIGIN      
        1 tggcgctggt tctgcagctc gacccggcca ccatccacat gtcgttcatc ggcgcggcgc
       61 tcgcggtgag ttatccgttc ttcaagcgct tctttccgct gccgcagttc tatctcggcg
      121 tggcgttcag ctggggcgtg cccatggctt tcatggcgca actcggcgag atcccgcgcg
      181 tggcttggat catctttttc gcgggcatca tctgggccgc catctacgac accatgtacg
      241 cgatggtcga tcgcgacgat gacctcaagc tcggcctgaa atcgagcgcc atcgtttttg
      301 gcgacatgga cacgctgatc atcggcgtgc tgcaggcgtt gatgctctac gcgttgtacc
      361 tggtcggcac cagcatggaa tacggctact ggtacaacgc cgggctggta ggcgcgggcg
      421 tgttcttcgc ctaccagcag tggcgcatcc gcgagcggaa gaggcagtcg tgcttcgacg
      481 cgttcctcaa caaccactac gtcgggtatt cggtcttcat cggcatcctg ctcgaatatg
      541 cgttccgcgt acccgagcag gcgctgattc agtgagcggc taccaccacg tgtagtagag
      601 cgcggcgaga atcacgacga tcagcccggc tgcgacattg aagaacgtgc cggtcctgaa
      661 gtcgacgttg ttgatgtcga cgcgcatcgc ggccgccttg ttcggcgaca tcagcgagat
      721 caccaccgcg atgacgatgc cggcaacgaa gcaccagccc acacggttca tgaacggata
      781 gtcgggcagg tatttgtaga acacccacga gatcaccacg gtggcgagca tggagaccat
      841 cgccgccgtt gccgtcgtgc gcgcccagaa caggcccagc aggaagatta cgcagatgcc
      901 cggggtgacg aagccggtga agttctggat gtactgaaag ccctggccga aattgctcag
      961 gaacggcttc gtgatgaaga ccgcgatgac caccgatacc accgcgacga tgcggcccac
     1021 cagcaccagc tttttctgct cggtgttcgg ccgtgcggcg cgatacaggt ccatcgtgaa
     1081 aatcgtcgcg atcgaattga tcttggagcc ggtcgacgca acgatggccg ccaccagtgc
     1141 cgcgaacacc aggcccttga tgccgatggg caacatggcc atgagactcg gataggcctg
     1201 gtcggtcttc tcgaggccgg gcacgagcac gagagcggcg atgccgggca gcacgatgat
     1261 cgcgggcatc agcagcttga ggaaggccgc gagcacgatg cccttctggg cctcgttcac
     1321 gctcttggcc gcgaggccgc gctggatgat gtattgattg aagccccagt acgagacatt
     1381 catcatccac atgccgccta acaacacggc gaggcccggc agatccttgt agtgcgggct
     1441 gtccggcgac aggatcatgt cgaacttctc ggggtgctgc gtggtgagga tcctgaatcc
     1501 ttccaggacg ccggcgccgc cgctgacctt gtcgagcgac atgtacgtga tgatgattcc
     1561 gcccagcacc agcagcgaca cctgcacgat gtcggtgagc gcgaccgctt tcagtccacc
     1621 gtacagcgca tacaacagcg cgaaaaccgc cagcgccgtg atcgcgacct ggatgtccag
     1681 gcccgcgatg gcgttcaccg ccagcgctcc gagccacagg atcgacgtga ggttcacgaa
     1741 gatgtacagc gcgagccaga agacggccat cacgttgcgc accgccgggc tgtaccgccg
     1801 ctcgaggaac tccggcatcg tgtagatgcc gttcttgagg aacaccggca ggatccactt
     1861 gccgacgacg atcagcgtga gcgccgccat ccattcgtac gaggcgatgg ccatgcccat
     1921 gacgtagccg gagcccgaca tgccgacaat ctgctcggcg gaaatgttcg ccgcgatgag
     1981 tgaggtgccg atggcccacc agggcagcgc acgactagcc aggaagtagt cctgcgcgtc
     2041 cttctggtgt gtgccttttt cgcgcgacac ccactgcgcc agtacaaaaa ttccgaccgc
     2101 gtagatcgcc aggatgacga tgtccagagt tgccagattc acgaaaaccc cctgctgctg
     2161 cggcgtcgat ggacgccgtt actttttttc catcgcatcg cgcattgcct gaaccaggtc
     2221 ctgcgcgcgt ttcgtcagct ccgcgagtgt atacgcgggc ttgaaaagtt caccgccaaa
     2281 tccgaagcca tccgcgcctg acgcgagcca tgtggcgatg tcctgcgagc caatgccgcc
     2341 gaccggaaag accttcacgt gtttcgggag cacggatttg agggctttga gatgtccggc
     2401 gccgtaactc gacgcgggaa atagtttgag ctcggtggcg ccgtggcgca ccgccgtgaa
     2461 cgcttcggtc gcggtagcga taccagggag cacgcgcagg cccagcccaa gcgcgtggcg
     2521 gatgacctcg ccgtcgcaat tcggcgcgac caccagccgt ccgccggcag cgtggaccct
     2581 atctacctcg gcgcgtgtga gcaccgtgcc ggcgccaatc aggcacgtgg cgccgtgcgc
     2641 ctgcgtcagc aattgaatgg tggtgaacgg atcgggggaa ttcagcggaa cttcgatggt
     2701 gcggaagccg caactcacga gcgcagcgcc gacttccggt gcccgcaccg gtgtgagccc
     2761 gcgcaggatg gccaccattt cgatcatgat ttctgtcgct ccagctcgag atgcacgaag
     2821 gtgagtccgg cgagggcggc cttgtcgccc tcgatgcaca cggtcttgcg tccgtggcgc
     2881 gcgagcgcgc gtgaatagga atcgttgagc tgtggcgtgc cgatgacgta gaccgatgcc
     2941 gcgcgaggca ggtccggaaa cagggacagc gctccggcga cgtccgagcc gatgagcaac
     3001 ccggacatcc acgatgcggc gccctccgcg ctgagctgct tgtcgatgcg gaggctgcgg
     3061 ccctggaaca gacggtgcag caggttcacc tgcggatgat gtgccgactc cgcgagccca
     3121 cgctcgaaat ccactggctg gtggacgatg ggcgtcgcct tgtcgcgcac cagcacactg
     3181 tgctcgcaca gcatggcgaa cagctcgccg gtgggcgcgg tcaggaattc ctgtacgacg
     3241 cccttgtgca gcgataccca cttcgtgtgt gttcccggca tgcagacgag ctgcgctccg
     3301 acttcgagcg atgcgtcgag atagcgtgca ccgagcagct gcgtctcctc gccgcgcatg
     3361 acgtccggcg ctccgagcgg gttactgcag cgcatgcccg agacgatgaa caccccgtcg
     3421 cgaacccgcg cgggagcttc cgcgagctcg tcgagctccg cgggacacgg tagatagggg
     3481 acttcgagcc agccgatggt cgagcccacc atccctgaca ataccgctgg cagttcacca
     3541 tgtgcgcgcc gccagtcggc gctgagcgtg tcgaagattt ccgcatgttt gccgcgcgcc
     3601 tcggccgcac ccgggccctt gcgcacgtcg agcgtcccgc cctcggcatc acagagcgtg
     3661 agccgcagat tgctcgtgcc ccagtcaccg gcgatgaacg ctgccaggtc tttcgatacc
     3721 tggctcatgg catgtacatg ccgccgttga cgtgcacgat gttgccggtg atgtagcccg
     3781 acaattcgtt cgacgcgaga aacaggaacg cgccggcgca gtcttccgcg acaccgacac
     3841 ggcccatcgc ggtggcatca cgcatcgcgt ccatgcgcgc ctgcggcgta cgcgcgtgaa
     3901 acggcgtggc gatggcgccg ggagccacgg tgttgacccg gatattcttc gccgcgagat
     3961 ccgtggccag atggcgcgtg atgttgtgca cggcggcctt tgccgttgca tacatgctcg
     4021 agcccggacc accgccatcg atgccggcga tggaccccac gttgacgatg ttgccgccac
     4081 cgcgcttctc gaaatgcgga attgcggcct gggaagcggc gatcacggag cgcacgttca
     4141 ggtcgtacac gaaatcgaac aacgcatcgt cgacgtcggc aatggggcgc cgttcgccaa
     4201 ggccaccggc gttgttgatg agcacgtcga gaccaccgag cgccgccgcg gcttcgttca
     4261 ccacccgctt tgcttcaccg cgcttcgaga ggtctccgcg gatcaatgag gcacccggcg
     4321 aacccggtgc gatctcggcc gccagagcgc gggcagcttc ttcatgcgcg ttgtagtgca
     4381 gcgccagctt tgcgccctgg gcagcgaaag cgcgcgccag cgccgcgcca atgccggagc
     4441 tcgcgcccgt gatgagcacg gccttgccat tcagatcttc gattttcgtc atgcgcgcca
     4501 tgttactgag agctgagcgc gcgctccatc gccagcgacc cgtcgggcct cagaagtttg
     4561 atcacgagct tgaacgcacc ttgctgggcg cgcgcgggca cgtcgcgctc gacggagagc
     4621 gcccccggcg ggttcaacag cgcgcctcca ttcgcaaact ccttgttgtc ggcttcgacg
     4681 agctgccagc tcacgcggta gccattcatc gggtagcgcg gcatctcctg ctcgccgttt
     4741 ggcttcagcg tgaatgcgaa tgccgtgggc ttgccatcgg cattcttcgt ccatcgcgtg
     4801 tcgaggtgcg cgggtgcatt cacctgcgcc catgcgaaat acgacggctt gcgctgacgg
     4861 ttctcgtcga cgatgccgtg ctcgagatat ccctgctcct ggccgggcca gaagtagcgc
     4921 cgcgatttgt agtcctggta acaccagaga atggcgcccg cgatccagtc gcgcttcgcg
     4981 agcagcggca gctgttcgcg aatgatggag actcgggtcg cgtcggcttc ggtggcgttc
     5041 tttgcgaaaa tccccgggaa tccgagctcc gagatgatga ccatcttgcc gggaaacagt
     5101 cggtcgacct tgtcgagcgc gggatcgagc gcctcgcgcg gcccatgcca cgcaccgaag
     5161 tactgattca tcatgaggaa gtcggcgtcg ttggccgcgg attcggcggc cgtcttcagc
     5221 ttcggaagat tgtcgtcggc cagcgtgacg ggccgctcgg gatcgagact cttgatgtag
     5281 tcgcgcatgg cgcggaagaa acggatgccg cccggtgagc ccgcggcgct ttcattgagc
     5341 acgctccacg cgaacacgct cgggtggttg ccgtattgct ctatcaactc gcgcatctgc
     5401 tgcttggcaa gttccaggta ctgcggattc gagagctgtt cctcgcttgc ctgccagatc
     5461 ggaatctccg ggatcagcag gatgccgcgg cgatcggcga aatccgcggc gcgcgtgccg
     5521 ggcggatagt ggaccggtcg cgtgagagtc atgttcagac tcttcatgtc ttcccaatcg
     5581 tgacgcagtg tccccgggga ttcggcgaga ccctcccacg gcgaatcggt gtgccgcgtc
     5641 atgccggtga ggcgcacacg ctggccgttg agcaacaagt gccggtcgcg gatgacgagc
     5701 tcgcgcagac cgaaggagtc gttggcgagt tcgatcgctt tgccactgga gtcgatcagc
     5761 gccaccgtca tgcgatacag gttgggatga tcgagatccc agcgattgat ctgcttcacg
     5821 ccaatcgcaa acgaaacttc gttggccccg gccttcagcg tcacgggctg cgtgtgctcc
     5881 ccggccgtct cgccattggg gccggtcacg gttgcgtgca gcgacaggcg ttgtgcgctt
     5941 cggctcacga gcgtcacccg gtccgtcacg gccgcgttca cgccatcgag cttcgaacga
     6001 atgaattgtt tgtcgatgcg tacgggccca tgcaccgaga gccacacatc gcgcacgatg
     6061 ccgccatacg cccaccagtc gtaccaggcg tctggcgcgc cgcgagcgcc gaagcccggg
     6121 atcgtgaaca tgcccggccg gttgtcgatg gcgacgacca gcaggttgtc atcacgcagc
     6181 tgcgaagtga catcgaatga gtacgcactg tatccaccct cgtgcgagcc gatctccacc
     6241 ccgttgagcc acacgcgggc cttgtagaaa gtggcgccga agtgcagttg cgcgattgca
     6301 tccgcgggta gtttgggaag gtcgaacctg cggaaatacc agcctgtgcc aagatagtcg
     6361 tagtccgcgc cttcacggtt ccaggtatgc ggcacggtca cgctgcgtgt tccagccgga
     6421 atcgccttcg gccagctcgc gagggtcgcg ccggcttcga cgcccttctc ggtccgaaag
     6481 gcccagtcgc gagtcaggtc gattcgcgtc gcggcgccgg ccgcggcaca gagttgcaaa
     6541 gccagcaggg cccagagcca gcgcgcggta gccatgtttc agatctcgtg tggtgcaacg
     6601 atgcggcgtt cgccgatgag gaatgcatcc gcgaccagct ggaatggccg ccagtccact
     6661 tccgacttgc cggcctcgca gagcgaggcg aaacgttcgt acaaccgcgg atattcgccg
     6721 gccatgctgg catcgcgggt tatcgctttg ccgtctatat acagctccgc accgccgcct
     6781 ctgagcttga gcttgccgcc ggtcgtcatg agatcgatgt cccagctctg ttcgccctta
     6841 tggcgaaaat cgaattccgc accgatctcc acgccgtctt cggtgcgcat gcgaacgttg
     6901 gccgcgatgg gcgattgctg attctcggga aactcgagca atgcagcttc gacacagaca
     6961 tccgtgggca ggatttcggt cagcaacgac agcgcgttga tgcccggatc gaatactccg
     7021 aagccgcccg gctcccagat ccagcgttgt ccgggatgcc actggtacac gtcttctttc
     7081 cagttgatct tgccgctcac gagtttccgc gatcgcagga attcgcgcgc cgcatccacg
     7141 ccagcggcaa agcgcgaatg ccaggtctgg aacaaggtgc ggccgcagcg tgcggcttca
     7201 tcggccagca gagcgatctg ccgcgtggtg gccgccggcg gtttttccag catcacgtgt
     7261 ttgcctgcgc gtaatgacgt gagtgccgca tcgaaatgcg cctgcggcgg cgtgcagatg
     7321 gaaatgcact ggagatcggg gaccgcggcc agcatggcct cgaggtccgg aaagttcgac
     7381 actccgtcga ccgtcgcgtt gcggctcgcg caggccgcga gcgtgaagcg cgggctcgcc
     7441 cgcagcgagg gtatgtgctg gtcccgcgcg atcttgccga tgcctaacaa tcccactttc
     7501 aaacgcatgc agtctccacg gtcaacggct gttccgcgcg cctattcagc cagaatggcc
     7561 ttgagaaaat ccgcggccgg tgtcagctcg tttcccttgc ggaagtcgaa cagcgtcgcg
     7621 ttttcccaat gcgatccggt accccagcgc gtggcgcagc tcgtggaaac ccaggccggt
     7681 tcccaataga cgacgcccac tccgccggcc tcccgcgtca tccgcaccac atcattcatg
     7741 aagtgctgct gcccgccggg cgtcgcagga taaccgcgca gcagcgagtc ttcacccagc
     7801 agattcgctg ccttgtcgcc ggcgtctttc aacgtccagg gatatgaaac ctcgaccaca
     7861 atgatgtcag ctttgaatcg ctgcctgacc gtggcgagcg ctgcgccggc ctgtttcatg
     7921 ttgaacgtgg accacttcgg gtagtagctg acaccgatgt agtcgaagtc gcgcacgccg
     7981 gcggccgtgg cggcggcgaa ccagggctcc acgttttccg gttgcgccac gtgcagcatc
     8041 actttcggcg atgtgccgtt cgcgtccttc gcttcacgca cggcgcgaat gccggcgttg
     8101 agaaccttcg cgttgcgcgc ccagtcgatg ggctcgccct tggtttccgc aggccgcagc
     8161 atctgcgtgt tgatctcatt gcccacctgg accatgtacg gcagcaatcc gcgggtgttc
     8221 aggcgctcga gcacgtcgcg cgtgtattcg tagacatgcg tcgccagttt gtcggtatcg
     8281 ccgatgtcag ccgcccaggc agcgggaatg gtttgtttct gcgggtccgc ccagtcgtcg
     8341 gaatagtgga agtcgagcag cacctgcata ccggcttttc gcgcgcgctc gatggaacgc
     8401 gtcacgtcgg cttccgtgct gtatttcgtc cacgtcgggt tgttccacaa ccgcaggcgc
     8461 acgagattgg cgccgctttc ggcgaacagc tcgtaagggt ctcgcagctt tccatcgagc
     8521 ctgaattgcg ctccgcagga ttcgacctcg ttcacatagg aaagatcgac gccggcaaac
     8581 cgctttggag aggtgtcagc ggcggtcgcg gcgagtgccg ttatgagcat ggagacaatc
     8641 actacgccgc gctcgcgaag cctggaattc atcgctcctc cagaaggggc taccccacga
     8701 aaaaggcgcg gagagcatag ccctccgcgc cacggttcac gattgcctgt ccgttcagaa
     8761 cttgaaggtg aaatcgagca gcgcgcgccg gccgtagaca tcgaacgagc ccaggcggct
     8821 ggtgttgttg tcacgggtga tgcgcagcgg ctcgtcggtg aggttgctga gctgcagacg
     8881 catgccgaac atttcgttca cctgccatga cgtgctgaag tccaggatgc gctccgtgcc
     8941 gagcgagcgc acatccgagc cattccagcc cgcgatgatc gagaacggcg agtgatattt
     9001 ccatcccagg cgcgcctcga tgcccgactt ctggaaccag agatcgatca ccgccgtgtg
     9061 cttcgcgtat ccctcgatgg gcagtggatc cgtgacgggg tagaactccg acacgtccgt
     9121 gtcgacgaag gcatagttcg tgtagatgcc gaaattcctg agtgcgccct cgcgcgcgaa
     9181 tggcgtctgg aaggtcaatt ccatgcccgc gatgtcgccg ccgtcgccgt tattcggttg
     9241 cgtcacctgg taagtgtcac cgccgataac ctccggaacc gtcgtgtatc caatgtgggt
     9301 ttccacgtcc ttgtagaacg cggccattgc gaacagggct tcgggcccga agtagaactc
     9361 gtacgacaca tcgaactgcg tggccaggaa cgggtcgagc agcgcgttgc ccgcctgtcc
     9421 cgtgaatggc tgcgcctggt tccagagcgt gcgcgaggcg cgcagctcat cgagcggcgg
     9481 acgcgcgatg acgcgggccg cgccgaagcg gagcaggttc gagtcggtca ggttgaacgt
     9541 caggttcaag ctcggcagcg cttcggtgta gtcgttgtcg tatgacagag gctgcgcggc
     9601 gccgccgttg aacgattgga atcccgagct cgtggtttcc gtgcgcacga aacgcacacc
     9661 ggcattgccg gtgaatggcg agcccatggt gccgccgaag cgcaacatgg cgtacgcctc
     9721 gctcaccgac tcttccacgt tccagccact ggtcggaacg acggcgctcg cgctcgccgg
     9781 catgccgccg tacacatagt cgacgatttc atcgaaatcg ccgttcagca gcggtggcgc
     9841 atcgaactcc ttgaccgaga agctggagaa caggctcgcc ggcagaggtc ctgcagcggt
     9901 accgatgaag tccgcctcgc gacgggtgta gtccttctcg cgctcggaaa agcgcactcc
     9961 gaattgcaat gctttcacag cgccttcgaa gcgacgagtg gcgtcgaaac gcacggcgga
    10021 cagttcgtcc gtgaggtcat cgggaccatc gctctgcccg ggtaaccagt tcggcgcagc
    10081 ctggatcatc ggatcggaag gatccgagct cgtggtgacc gacggacgta cgccggcgct
    10141 catgtcatag gtcatcgtcg ccggccagac ctccgacatg aatgctgccc agcgattggt
    10201 gcgctgcgca tcggagtacg agagatcacc gctcaccgtc cacgattcgg tttcgaactt
    10261 gccattgacg ccggtgacca gcaggtcctt gtcctcggtg tacttcgcga tgacgttggt
    10321 gacgggtgcg aaacagttgt cgagcgtggc tccgacgaca ctgccgttct cgacggtgta
    10381 cgaggactgc gcaccgtcgt agcaccatgc ctggccgttg tcccagttgc cccagaagtt
    10441 gcggccgtac caggcctggt tctggtcttc atcgatggtg aacttcgaat acagcgcatc
    10501 gacgttgagc tcgaagctgt ccgtcggacg ccagccgatg gcggcgttca caccgtaacg
    10561 gtcttcggtg agtttcttga cctcggtttg cgcgccccac ggcgtcggat cgatctctcc
    10621 gtcgccatcg atgtcgccgg tggcatcggt ggagtgcatg gattcgtcgt tgtatcccca
    10681 gccctggaac gaagggaacg cattcttctg ccgctgcgcg ctggcgccga ggttgaacgc
    10741 gaagttgtcg ccgacggaat gggtgaagct ggcgctgccg cgataaccgt aggggtcgta
    10801 gcccgggatg tccgcgccgg cttcgtaata aaccggccct ccccgcaaca ggatctccgg
    10861 ccctgtgtag ttgagcggct ggatggtctt gatgtcgatg gtgccggcaa cgccgccagc
    10921 cacgatatcc gccgactgcg acttgtacac gtcagcgccg gaaacgactt ccgagggata
    10981 gatttcccag cgaacatttc gattcggctc ggaggacgcc acttcgcggc cattcaccag
    11041 gccgagcacg aggcggggac caagccctcg caatgtcgcc tggctctggt tgccgcggtc
    11101 acgcgtgccg ttgacgccgg gcaggcgcac cagtgattcg gcgatggtca cgtcaggcag
    11161 ggcgccgatg tcgccgccgg aaatggcgtc caccaccagc tccgagcttc gcttcacctc
    11221 gagcgcgctc tgcaggctcg cccggtatcc gctgacgacg atttcaccga tttcttcggc
    11281 ctcggctttc tgagccggcg cctcctggct catggcaacc ggagccgtga ttagcgccgc
    11341 catggaagcg agcgcacgac gatgtgcttt cgagtgcatc tgaatttccc cccaaattag
    11401 tgccaagtga ccggttcaag ccgggcacgt ctgctgcatc aaaacaacaa caaactgatt
    11461 aatagtatca gtatgggcag aagcacatta atgcgtcaac aacagcgcgg gggttacggc
    11521 gctgcgatgc cgctgcggat atagatagct cgaaaaccgg aatcccgcgg cttcaggtcc
    11581 gggctttgtc cgtcttgatg acgagcatga gcgcttcctt gatgagctcg cggcaggcgc
    11641 ggaaagcggc ttccgaattg ccattgcgga tcgcgcgata gattttttcg tgcgcgtcgt
    11701 aatctggcaa cgttcctttc accttgttgg tcaggcgaat ggaaaagcgc agcgccgttt
    11761 cgatgatggg cgtgagctgg tgaaagaaac ggttgtggct gatctcgaga atggtggcgt
    11821 gaaacgcgat gtcggccgac acggggtcga aatgcccgtc gttcgcctgc ttcatcttct
    11881 ccagtttctg cttgagcaat tccttgtctt cgtcggtcgc attggtggcg gccagcgccg
    11941 cggcagccgg ctcgatgccg atgcggactt cgagaaattc gcgcaccagg cggtgcgaga
    12001 agtggcggtc gagaatccat ttcaggacct cggcatccag caggtcccat ttctcttccg
    12061 gatgaacata agtgccgacg cgcggccgct gaccgatgag ccccttggtg gagaggatct
    12121 tcaccgcttc gcgcatgaca gtacggctgg cgccaaactt cttgccaagc tccgcctcga
    12181 tgggcagcgg tttgcccgcg ggcaattcgc cgcggacgat ggacgacccg agcttctgca
    12241 cgagcgactg ggtcagattg atggttccgg acatgccctt acgctatcac gacaacacgc
    12301 gcgggacgga atattaatca gattattcgc cggcggggag cccgctggca cgggacaggc
    12361 ggatgtctcg tccgggacag gtgcgggtgc gccaggcaat gcgcaccgcc caatacctgg
    12421 tagttaggcc gccggactca caccgcttcg gtgacgccca gctccctgag gatcgccacg
    12481 cgcagcgcct gcgcctccgg actggacacg cggcgtggat gcgcgaatgg cacgtcgaat
    12541 gaagcctgga tgcgtgccgg ccgcgggctc agcaccagga tgcgatcggc gaggtggatg
    12601 gcctcttcga cgtcatgcgt gatgagcatg acggtgtgct tctcgatggc caggatgcgc
    12661 agcagctcgt tctgcatgcg caggctcatc agcgcgtcga gcgccgagaa gggctcgtcc
    12721 atgtagagaa tctcgggctt gaccaccagc gcgcgtgcga tctcggcgcg cttcagcatg
    12781 ccgccggaga gttcgtgcgg atagttgttt tcgaatccct tgagtccgac cagcgccgcg
    12841 tattcgtcgg cgagcgtttc gcggttgggc acgtcgtcag gcaggccgaa cagcaggttc
    12901 tgtttcacgg tgagccacgg aaacaccgag ccctgctgcg tgatcatgat gcccttgcga
    12961 ttcggcccgt tacgcaccac gccgttcacg ttcacggtgc ccgagtcggg agcgaggaac
    13021 cccgcgatga tgttcatcag cgtggtcttg ccgcagcccg acgggccgac gatggcgacg
    13081 aactcgccat cgttcacctt gaagtcgagg ccctcgatga cctgcagacg caccttgtcg
    13141 ttctggaagc tcttgcggac gtcgttgacg tgaatcttag tgggcataac gccaccgcac
    13201 gactttcatg ctctccagca acctcatgaa cccgtcgagc atcaggccga tgagcccgat
    13261 gatgatcatg ccggcgatga ccaggtcata acggttgccg gcgttgcgcg aatcgatgat
    13321 gagatacccc aggcccgagc gcagcgcgat catttcggcc gccaccacca ccagccaggc
    13381 gacccccagg ccgatgcgca tgccgacgat gatctgcggc agcgtcgccg ggaacaccac
    13441 cttcaggaac agcttcgagc gcgatacgcc gaaattttcc gcggcgcgca ggaagcgctt
    13501 ctcgatggtg tgcactccaa cggtggtctg cacgatcatc ggaaacaccg acgcgatgaa
    13561 gatgaggaag ataggcgagg cgttgcccac gccgaaccac aggatggcaa tcggaatcca
    13621 ggctatgggg gaaatgggcc gcagtatctg gaacagcggg ttcagcgtga cgaacgcgcc
    13681 atgcacccag cccatccaga ggccgagcgg tatggccagc gccaccgcga gcccgaaacc
    13741 ggcgcccacg cgcatgagcg acgcgccgat gtgctcccac aacgtgccgt cacgcgcgag
    13801 ttcgagcgtg cccgccacca ccgcccaggg cgttggaaag atcgcgctct cggtagcaat
    13861 cacgatggcc caccatattc cgaccaccac cgcgatcacg acgatcgacg gcagcgcctg
    13921 cttgagaccc ttcatctgct tttgttctcc caccattcac gaacccgcca gcgaaccttc
    13981 gaccagggca tcgccgggta cggctccacc agtaccgtca tctgcccgct tgcatgcgcg
    14041 gtgcacgcga agtcgtaact cgccgctttt tcaaacggca accggaagct ttggcccggc
    14101 atcatgatcg tgggcccgaa gacctgcggc acatcatcga ggttgcgcag catcagcacg
    14161 tcgttgagtc cgagtgtcag gcggatcttc gacggaagaa tttccacctt gttgcccgcc
    14221 atgcgccgcg cgtacgtgcc cttcggaatt tcgaacaact cgtcgcgcga aggcacgctc
    14281 accggcgcga agcccgccca tccgagaacc gccagcaccg cgagcagcgg caccgcaatc
    14341 caggctcgcc tgatcgaact
//
LOCUS       DASQJS010000386         4400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2761903, whole genome shotgun sequence.
ACCESSION   DASQJS010000386 DASQJS010000000
VERSION     DASQJS010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2761903"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1291)
                     /gene="lptG"
                     /locus_tag="VEW08_15190"
     CDS             complement(224..1291)
                     /gene="lptG"
                     /locus_tag="VEW08_15190"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HYJ42137.1"
                     /translation="MNILDRYVIRAVLGGVLVVLAVLVTLGALFLFSGQQDDIGQGSY
                     TTLDAFWFVLLNVPQQVYETMPIAVLIGALLGLGTLSRGSELTVMRAAGLSVWRITGS
                     VAIAGLALVLFAVICGEFLAPPLQAMAKQQKLLAKFSTITFAGRGGPWVRDGDLLINV
                     SQQSGGGEFGGMRIIELTPDHELKSVATASTATVEEDGSWLLSHYANTTFGGERIESD
                     HQETRTFQSAVGGDFLALTVSSPRQLETRVLWNLIRHLQQNDLDATEQTFAFWSRIAR
                     TTAILFATLLAVPFVFGSLRSAGAGARTLLGVMIGVLFFLVQRMLESGAVVFDASPVI
                     LAWVPTALLGSAALILIARTR"
     gene            complement(1288..2409)
                     /gene="lptF"
                     /locus_tag="VEW08_15195"
     CDS             complement(1288..2409)
                     /gene="lptF"
                     /locus_tag="VEW08_15195"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HYJ42138.1"
                     /translation="MLRILDRYILREVAMQWLSVSGVLLAIMLTDQVARVLERAAEGG
                     FPRGVVFELILLGATRSLPMVLPIALLLGIVLALGRLYHDSEMTAAQACGTGSRIVMV
                     PVIAFTLVLTALLAYVSLQVAPAAAARMLALSNEAMRAGQFAPISAGKFSTFGGGGTV
                     VYAQAADADGTLRRVFVQRSRGNVLEIALAQRATHTYSEGGDIQVITLFDGERYEGIP
                     GERHFRIVRFAENTIPVRLPAMSGGVMKLDGVPTSELFASTKPDLRGELHWRLALPIM
                     AVVMGLIAVPLARLRPRQGRYARVGYAILIFFVYIELAIAGKVWLVRGVTPEWLGLWW
                     VHGAVVLFAATLLLVPRWLARARHRRNMARSAVHATAAT"
     gene            2486..4006
                     /locus_tag="VEW08_15200"
     CDS             2486..4006
                     /locus_tag="VEW08_15200"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HYJ42139.1"
                     /translation="MQFEIWNKGLAELSIDCLAVGIHDDGELTPEAKVLDLRCREKLS
                     RLVKRGDFTAKAGETWLVTDLDGIEAERVLLVGIGPKAPSSTEAGARKSWRRSVAAAV
                     GAATRTRIGALALALPRPAAKLLSDERLGRAVAEITGHTLYRVNDLKSGKKPRAHALS
                     RIVVAATAKAEASIKKGFTQGSALASGAALMRNLANLPGNVCTPSYLAKTAQDLARTY
                     KSLRVKVLGLPEIKREKMGCFLAVTQGSEEPPRFLVIEHRHSRAQNKNNSGPTVLVGK
                     GITFDTGGISLKDPPTMDEMKFDMSGAACVIGTMKAVAELNLPLNVIGIVAACENMPD
                     GRAIKPGDIVTSAAGLTVEILNTDAEGRLILCDALHYARRFKPDVVVDMATLTGAIVV
                     ALGTHHTGVFANNDALARELVECGAAVDDRAWQMPLTEEYGEQLKSNFADMANVAGRD
                     GGSITAAAFLAKFTKDLTWAHLDIAGTAYQGGAGKGSTGRPCAMLLEFLLRRASAR"
     gene            4038..>4400
                     /locus_tag="VEW08_15205"
     CDS             4038..>4400
                     /locus_tag="VEW08_15205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HYJ42140.1"
                     /translation="MPPRIDFYVSEAAGADARLRLACRVAEKAYLAKQTVVVLIDDPE
                     ALRRFDEMLWTFGDGTFVPHDAVTAEGAQCEAPVALTTGPLPAGHADVLLNLGNTVPD
                     RFEKFARVAEFLDSRPEVR"
BASE COUNT          859 a         1517 c         1351 g          673 t
ORIGIN      
        1 cgatccctgc gtcttcggga ctcttcgcca tcctgagatc caaccaccca ctaaggtgtc
       61 ttcgcgtgtg tcggcggccc gcaaactagc gatgtcaccc tgaacgaagg gcccggcgaa
      121 aagcgagtct catcgcatgc agggccgccc gtcgctcgac cctccactta cgaaccccgt
      181 cccgatttgc cagcaaaaga ggaatgtccc catttcctgg tggtcagcgc gtccgcgcta
      241 tcaatatgag cgccgcgctt cctaacaacg ccgtcggcac ccaggcgaga atcaccgggc
      301 tggcgtcgaa aaccacggcg ccgctctcga gcatgcgctg cacgaggaaa aacaggacgc
      361 cgatcatcac gccaagcagc gtgcgcgcac ccgcgcccgc cgagcgaagg ctgccgaaaa
      421 cgaagggcac ggcaagcagc gtggcgaaca ggatggccgt ggttcgcgcg atgcgcgacc
      481 agaacgcgaa ggtctgctcg gtcgcgtcga gatcgttctg ctgcaggtgc cggatgaggt
      541 tccacaacac gcgggtttcg agctgccggg gcgaggacac ggtcagggca aggaaatctc
      601 caccgaccgc cgactgaaat gtccgggttt cctggtggtc ggactcgatg cgctcgccgc
      661 caaacgtggt gttggcgtag tgcgaaagca gccagctgcc atcctcctcc accgtggcag
      721 tactcgcggt ggccacggat ttcagttcgt gatccggcgt gagctcgata atccgcatgc
      781 cgccaaactc gccgccaccg gattgctgcg acacattgat gagcagatcc ccgtcgcgta
      841 cccacggacc gccgcggccg gcaaaagtga tggtggagaa cttggccagc agcttctgct
      901 gcttcgccat ggcctgcagc ggtggcgcca ggaattcgcc gcagatgact gcgaacagca
      961 ccagcgccag gccggcaatg gccactgaac ctgtgatgcg ccacaccgaa aggccggccg
     1021 cgcgcatcac cgtgagctcg ctgccgcgcg acagcgtgcc caggccgagc aacgcaccga
     1081 tgagcaccgc gatgggcatc gtctcgtaga cctgctgcgg cacgttcagc aacacgaacc
     1141 agaacgcgtc gagtgtcgtg tacgagccct ggccgatgtc gtcctgctga ccggagaaca
     1201 ggaagagcgc gcccagcgtc accagcaccg cgagaactac cagcacgccc ccgagcaccg
     1261 cgcggatgac atagcgatcg aggatgttca tgtggcggcc gtcgcgtgca cggcactgcg
     1321 cgccatattg cggcgatgtc gcgctcgcgc gagccagcgg ggcaccagaa gcagtgtcgc
     1381 ggcgaacagc acgaccgcgc catgcaccca ccagagtcct aaccactcgg gcgttacgcc
     1441 gcgcacgagc catacctttc cggcaatggc gagttcgatg tagacgaaaa agatcaggat
     1501 ggcgtagcca acgcgcgcat agcgcccctg gcgaggacga aggcgcgcga gcggaacggc
     1561 gatcaggccc atgaccaccg ccatgatcgg caacgccagc cgccagtgaa gttcgccgcg
     1621 cagatccggc ttggtcgatg cgaagagctc actcgtagga acgccgtcca gcttcatgac
     1681 accgcccgac atcgccggca gccgtaccgg gatggtgttc tcggcgaagc gcacgatacg
     1741 aaagtgacgc tcgccgggaa tgccttcata ccgctcgccg tcgaacagcg tgatcacctg
     1801 gatgtcgccg ccttccgaat aggtatgagt ggcgcgttgc gcgagcgcga tctcgagcac
     1861 gttgccgcgc gaccgctgca cgaacacgcg gcgcagcgtg ccatccgcgt ccgctgcctg
     1921 cgcgtacacc accgtgccac caccgccgaa ggtgctgaat ttgccggcgc tgatgggcgc
     1981 gaactgcccg gcgcgcatcg cctcattgct gagggcgagc atgcgcgccg cggcggcggg
     2041 cgcgacctgc agggagacgt aggccagcag tgctgtcaac accagggtga acgcgatgac
     2101 cggcaccatc acgatgcgcg agccggtgcc gcaggcctgt gctgccgtca tctcgctgtc
     2161 gtggtagagc cgcccgagcg ccagcacgat tcccagcagc agggcgatcg gcaacaccat
     2221 gggcaggctt cgcgtggcgc ccagcaggat gagttcgaac accacgcccc gcgggaaacc
     2281 cccttccgct gcacgctcta acactcgtgc aacctggtcg gtgagcatta tggccagcaa
     2341 tacgccggat accgacaacc attgcatggc gacttcgcgc aggatgtagc gatcgagaat
     2401 tcgcagcaac agaccaccct tccgacttga gttacacttc gcgaccttaa aacaccccac
     2461 cctccgcgac aatgcgagcg tgatcatgca gttcgaaatc tggaataagg ggctcgccga
     2521 gctttcaatc gattgtctgg ccgtcggcat tcatgacgac ggcgaactca ccccggaagc
     2581 caaggttctc gacctgcgct gccgggaaaa actctcccgg ctggtcaagc gtggtgactt
     2641 caccgccaag gctggcgaaa cctggctcgt caccgatctc gacggaattg aagccgaacg
     2701 cgtgctgctg gtcggcatcg ggccgaaagc cccttcttcg acggaagcgg gcgctcgcaa
     2761 atcctggcgc cgttccgtcg ccgccgccgt cggcgcagcc acacggacgc gcatcggcgc
     2821 actggcgctg gccctgcctc gccctgccgc caagctgctc tccgacgaac gcctgggccg
     2881 cgccgtggcc gagatcaccg gacacacgct gtatcgggtc aatgacctca agagcggcaa
     2941 gaagccgcgc gcacacgcgc tgtcgcgcat agtcgtcgcc gccaccgcga aggccgaggc
     3001 gtccatcaag aaaggcttta cccagggcag cgcgctggcc tccggcgccg cgctcatgcg
     3061 caacctcgcc aaccttcccg gcaacgtctg cacgccctcg taccttgcga agacggcgca
     3121 ggacctggcc aggacttaca aatccctgcg cgtgaaggtg ctcggcctgc ccgagatcaa
     3181 gcgcgagaag atgggctgct tcctcgccgt cactcagggc agcgaagagc cgccacgctt
     3241 tctcgtcatc gaacatcgcc actcacgtgc acaaaacaag aacaacagcg ggcctaccgt
     3301 actggtcggc aagggcatca cgttcgacac cggcggcatc tcgctcaaag acccgccgac
     3361 catggatgag atgaagttcg acatgagcgg cgcagcctgc gtcatcggca ccatgaaggc
     3421 ggtggccgag ctcaacctgc cgctaaacgt catcggcatc gtggccgcct gcgaaaacat
     3481 gcccgatggg cgcgccatca agccgggcga catcgtcacc agcgccgccg gtctcaccgt
     3541 cgagatcctc aacaccgacg ccgagggccg cctcatcctg tgcgacgcgc tccactatgc
     3601 gcggcgcttc aagccggatg tggtcgtcga catggcgacg ctgaccggcg ccattgtcgt
     3661 cgcgctcggc acgcatcaca ccggtgtctt cgccaataac gatgcgcttg cccgcgaact
     3721 cgtggagtgt ggcgccgcgg tcgacgaccg tgcctggcaa atgccgttga cggaagaata
     3781 cggcgagcag ctcaagagca acttcgccga catggccaat gtggccggcc gcgatggcgg
     3841 ttccatcacg gccgcggcgt tcctcgccaa gttcaccaaa gacctcacct gggcgcatct
     3901 cgacatcgcc gggactgcct accagggtgg cgccggcaag ggcagcacgg gccgcccgtg
     3961 cgccatgctg ctcgagttcc tgttgcggcg agcatccgcg cgttagaggg tactccccaa
     4021 cggcgcgttc aaacccaatg cctccgcgaa tcgacttcta cgtgagcgaa gccgcgggcg
     4081 cagatgcgcg ccttcgtctg gcctgccgcg tcgccgaaaa ggcttatctc gcgaagcaaa
     4141 ccgtcgtcgt gctgatcgac gaccccgaag ctctgcgccg tttcgacgag atgctctgga
     4201 ccttcggcga cggcacgttc gtgccgcacg acgcagtcac ggccgaaggt gcccaatgcg
     4261 aagcgccagt cgcgctgacc acgggcccgc tgccggccgg ccacgccgac gtgctcctca
     4321 acctcggcaa caccgtgccg gaccgcttcg agaaattcgc gcgcgtggcc gagttcctcg
     4381 attcgcgacc cgaggttcgc
//
LOCUS       DASQJS010000387         7144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4647469, whole genome shotgun sequence.
ACCESSION   DASQJS010000387 DASQJS010000000
VERSION     DASQJS010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4647469"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..609
                     /locus_tag="VEW08_15210"
     CDS             49..609
                     /locus_tag="VEW08_15210"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HYJ42141.1"
                     /translation="MNMKFAAVAALGALSLAAIAPQAAAADNGFYIGAGITTTDFDMS
                     IEDFGSETLDDNGFKVIAGFRPLDWLAVEVNYMDLGDAEFDDGSEVTIESRAITASAL
                     FIKEWQVIDIYARLGMAKWDSDFNAPAGTVSDDGWEPTYGVGIGAHFGSVGIRAEWEQ
                     FSTEALDDFLETDLSTISLSVTYTFL"
     gene            complement(872..1465)
                     /gene="yihA"
                     /locus_tag="VEW08_15215"
     CDS             complement(872..1465)
                     /gene="yihA"
                     /locus_tag="VEW08_15215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HYJ42142.1"
                     /translation="MSQFPKVRFLLSAASEEQFPADVGTEVLFAGRSNSGKSSAINTI
                     LVRNGLARASKTPGRTRLLNFFEIEEGRRILDLPGYGYAEASPVEKKQWIALIQKLPK
                     RTCISGLFLIVDIRRGIKDQDVQLLDWADASGWPIHVLLTKADKLNQRDRAAGLKTAE
                     EILGEGVTAQVFSATDKIGVQAAQKRLVELLAGPPSL"
     gene            1534..2202
                     /locus_tag="VEW08_15220"
     CDS             1534..2202
                     /locus_tag="VEW08_15220"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYJ42143.1"
                     /translation="MIESRFSLSLAAALVCMSLGFAHSATAADALVGSADAGATKAAV
                     CTACHGVNGNSSNPEWPNLAGQNAAYTREQLAMFKARKRINEIMYPIVKDMSDQDIAD
                     VAAFFSAQTPTGLEADPSYWKAGEALYKSGDPARNIPACTACHGPSGQGVAGAGYPAL
                     RAQHSVYTVKQLQDYLTKNRYRDATDAAVVNQTRNSAMMTTIAARLSPEDIRNLASYL
                     QGLR"
     gene            2221..2913
                     /locus_tag="VEW08_15225"
     CDS             2221..2913
                     /locus_tag="VEW08_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HYJ42144.1"
                     /translation="MTTRRALIPLVVLLAGSWTLNACARAPEPAAAAAAAALSKWQEG
                     TNYKLAPAPQPPGVAAGKVEVTEVFWYGCGHCYALDPALESWNAKKPPYVEFVRVPVI
                     WGPVQRQHAKLYYTIQALNRADLHTKVFDAIHQQGLPLSARDEVEARAMQLVFLSSQG
                     VNESRFNSAYDSMSVASNMLRAEEFTKKFAVANVPLMVVNGKYITSVSEAGSEAKLLA
                     LVNDLAASEKAR"
     gene            2938..3777
                     /locus_tag="VEW08_15230"
     CDS             2938..3777
                     /locus_tag="VEW08_15230"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HYJ42145.1"
                     /translation="MLSAFVPQPQGLARFELRSGAAIPGESIWLDLVEPTPSEEKQVE
                     SFLSIDVPTRDEMREIEASNRLYEEDGALYMTATVVTKLDSDLPESTQITFILSAAKL
                     VTNRYVDPLPFRRFIAYAERHPNSCNTPPSVLAGLLESIINRVADVIERVATDLDGAS
                     VEVFSASRRKRGVMRDFRKVLERLGQNGELISKARESLVSLGRLLTFVQQSTAVPMSQ
                     DVRARFRTQTRDVMAMSDHASFLGTKVSFILDATLGLINIDQNNILKIFSVVTVFLLP
                     PRS"
     gene            3792..3911
                     /locus_tag="VEW08_15235"
     CDS             3792..3911
                     /locus_tag="VEW08_15235"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42146.1"
                     /translation="MNFSKMPWLHETWGFWAAMGVMLASALGPFLFFKRKGWL"
     gene            3935..4011
                     /locus_tag="VEW08_15240"
     tRNA            3935..4011
                     /locus_tag="VEW08_15240"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3969..3971,aa:Arg,seq:ccg)
     gene            4043..4555
                     /locus_tag="VEW08_15245"
     CDS             4043..4555
                     /locus_tag="VEW08_15245"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYJ42147.1"
                     /translation="MSKQDSHFFNNFSLVLGILVAVTIGLFAFARHVGATHQGKQVTT
                     DPRYVQSVEERLQPVSRVAVAGQDNSALKIESAGPPVAAAALPADGTAAYETACAACH
                     GAGIAGAPKVGDKGAWGPRMAQGKETLYNHAIHGFNGKTGVMPAKGGRADWPDDLIKQ
                     AVDHMLELNQ"
     gene            complement(4561..6123)
                     /locus_tag="VEW08_15250"
     CDS             complement(4561..6123)
                     /locus_tag="VEW08_15250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HYJ42148.1"
                     /translation="MGIARVSSRAQLGLAAPRVEVEVHLGAGLPVFSIVGLPATAVKE
                     SKDRVRAALQNSDFEFPAGRITVNLSPADLPKEGCRYDLPIALGILLASEQVDARHES
                     LERTEFYGELGLAGELKPIKGVLLAAAHAARDEHHVVVPAGNLGEACLVAPAAARGAA
                     TLLDVCALLGPVAAGGGSVEALAAPGHPAPSVECVAPDLADVRGQAAGKRALLVAAAG
                     GHSILLIGPPGTGKSMLAQRLPGLLPTLSADEALDVASIASVSTRGFNPLDYGRRPFR
                     APHHTASASAIIGGGPHARPGEVSLAHRGVLFLDELPEFNRRVLESLREPLETGVVAI
                     SRAALQVDYPARFQLIAAMNPCPCGYLGETSGRCRCAPPEIARYRARISGPLLDRIDL
                     RVEVPTLPSEELLGERAPADAALTSAIAARLVRNTRELQHRRTGKLNADLSGSEIENL
                     CRLDRRCRLLLGQARVRFMLSARGVHRVLRVARTIADLEMISLHNETGPQDRSIGPVH
                     LAEALQLRRANE"
     gene            complement(6195..6458)
                     /locus_tag="VEW08_15255"
     CDS             complement(6195..6458)
                     /locus_tag="VEW08_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008091866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HYJ42149.1"
                     /translation="MEIPRVEELLRSLMQGLPPGLASGAGTLREDLENNFRAVLRANL
                     GKLDLAGRDEFDAQRKVLERTRARLEALEKRVAELEARANSGR"
     gene            6699..7037
                     /locus_tag="VEW08_15260"
     CDS             6699..7037
                     /locus_tag="VEW08_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HYJ42150.1"
                     /translation="MKMITAIIRPWKLDDLQMAVARLGVQGITVTEVTGYGRQRGHTE
                     HFRGAEYSIEFVPKLRVEIVLDTASAEAVIEAISNVCRTGKTGDGKIFLAPVGEAVRI
                     RTRESGAAAA"
BASE COUNT         1344 a         2342 c         2175 g         1283 t
ORIGIN      
        1 taaacctgcg ctctaatcca tcgtcctaaa actctggaga ggtctcccat gaacatgaag
       61 ttcgctgcgg tcgccgcgct cggtgcgctg tcgctcgccg ccatcgcccc gcaagccgcc
      121 gctgccgaca acggcttcta catcggcgcc ggtatcacca caaccgactt cgacatgagc
      181 atcgaagact tcggctcgga aacgctcgac gacaatgggt tcaaggtcat tgccggcttc
      241 cggccgctcg actggctggc agtcgaagtg aactacatgg accttggcga tgcggaattc
      301 gacgatggca gcgaagtcac gatcgaatcc cgtgccatca cggcttcggc gctgttcatc
      361 aaggaatggc aggtcatcga catctacgcc cgccttggaa tggccaaatg ggattcggac
      421 ttcaacgctc ccgccggcac ggtgtcggac gacggctggg aacccacgta cggtgtgggc
      481 atcggcgcgc atttcggtag cgtcggcatc cgggcagagt gggagcagtt ctcgaccgaa
      541 gcgctcgacg attttctcga gaccgacctg agcaccatct ccctgagcgt cacatacact
      601 tttctgtagc gcaggcgcga caggtcgcgt cgtaaccaca tgattttgta gcagtacttt
      661 tttaaatatt ttggaacctt tcggcgggtc cccgtgtctc attaaccgag cgcgtaaaac
      721 cgctccacgc ttccctccct gagcgtgtag cccggttcac gagacctgtg ccgggttagt
      781 ctggccccgg tggttcattc caccggggct ttctttttgg ggctccgccc acgtcggaaa
      841 acgccgccga agcggcggcg tatacgagtg tttaaaggga aggaggcccg gccagcagct
      901 ccaccagccg cttctgggcg gcctggacgc cgatcttgtc cgtggccgag aacacctggg
      961 ccgtcactcc ctccccaaga atttcttcgg ccgtcttgag tcccgcggcg cgatcccgct
     1021 gattgagctt gtcggccttg gtcagcagca cgtgtatcgg ccagcccgag gcgtcggccc
     1081 agtcgagcaa ctgcacgtcc tggtccttga tcccgcgccg gatatccacg atcaggaaca
     1141 acccgctgat gcaggttcgc ttcggcaatt tctggatgag cgcgatccac tgcttcttct
     1201 cgaccgggct cgcctcggcg taaccatatc cgggcagatc gaggatgcgc cggccctctt
     1261 cgatttcgaa gaaattcagc agtctcgtac gcccgggagt cttgctggcg cgcgccaatc
     1321 cgttgcgcac gagaatcgtg ttgatggcgc tcgacttgcc agagttcgag cgcccggcga
     1381 acaggacctc ggtgccgacg tccgcgggaa attgctcctc actcgccgcc gaaagcagga
     1441 agcggacttt gggaaactgg ctcatgggag gctacgcttg agtcgacaca cgctagtgta
     1501 caattcgccg ccttccaggg agcccgcaag accatgattg agagcagatt ttcgttgagc
     1561 ctggccgccg cacttgtttg catgagcctg gggttcgccc actccgccac cgcggccgat
     1621 gcactcgtcg gttcggctga cgccggcgcc acaaaggcag ccgtgtgcac ggcctgccac
     1681 ggagtgaacg gcaacagcag caatccggag tggccgaatc tcgccggcca gaatgcggcc
     1741 tatacgcgcg aacaactcgc catgttcaag gcgcgcaaac gcatcaacga aatcatgtat
     1801 ccgatcgtga aagacatgtc cgatcaggat atcgccgatg tcgccgcctt tttctcggcg
     1861 cagacgccca ccggtctcga agccgaccct tcttactgga aggcgggtga agcgctgtac
     1921 aagtcggggg acccggcgcg caacattcct gcttgcaccg cttgccacgg cccgtccggc
     1981 cagggcgtcg ccggcgccgg ctaccccgcg ctccgcgcgc agcattcggt ctacaccgtg
     2041 aagcagttgc aggattacct cacgaagaat cgctaccgtg acgccaccga tgcggcggtc
     2101 gtcaaccaga cgcgcaacag cgcgatgatg accacgattg ccgcgcgcct gagtccggaa
     2161 gacatccgca atctcgcttc gtatctgcag ggcttgcgct aactaccacg gaggccgctc
     2221 gtgacgactc gccgcgcact cattcctctc gtcgtgctgc tggccggcag ctggaccctg
     2281 aatgcctgcg cccgagcccc cgagccggcg gcagccgcag ccgcagccgc tctcagcaag
     2341 tggcaggaag gaactaacta caagctcgcg ccggcgccgc aacccccagg cgttgcggcc
     2401 ggcaaggtcg aggtcactga agtcttctgg tacggctgtg gacactgcta cgcactcgac
     2461 ccggcgctcg aaagctggaa cgcgaaaaag ccaccctacg tggaattcgt gcgggttccg
     2521 gtgatctggg gtccggtgca gcgccaacac gccaagctct attacaccat tcaggcactg
     2581 aatcgggccg atctgcacac caaggtgttc gacgccattc accagcaagg tctgccgctt
     2641 tccgcgcgtg acgaagtcga ggcgcgagca atgcaattgg tcttcctcag ctctcagggc
     2701 gtcaacgaaa gccgattcaa ttccgcatac gactcgatgt cggtggcatc caacatgttg
     2761 cgggccgagg agttcacgaa gaaattcgcc gtggcgaacg ttccgctcat ggtcgtgaac
     2821 ggcaagtaca tcaccagcgt cagcgaggcg ggatcggagg ccaaactgct ggccctggtc
     2881 aatgatctcg ccgccagcga gaaggcccgt tagcggttca ctccgcccct ctccgacatg
     2941 ctgagcgcgt tcgtccccca accccaaggc ctcgcgcgct ttgaactgcg ctccggcgcc
     3001 gccattcccg gcgagtcgat ctggctcgat ctcgtcgagc ccacgccgag cgaagagaaa
     3061 caggtcgaaa gtttcctgag catcgatgtt ccgacacgcg atgaaatgcg cgagatcgaa
     3121 gcgtcgaacc gcctctacga agaggatggc gctctgtaca tgaccgccac cgtagtcacg
     3181 aagctcgatt ccgacctgcc ggagagcacg cagatcacat tcatcctgtc cgccgccaag
     3241 ctggtcacca atcgatacgt ggacccgctg ccattccggc gattcatcgc ctacgcggag
     3301 cggcatccga attcctgcaa taccccgccc tcggtgctcg cgggcctgct cgaatccatc
     3361 atcaatcgcg tcgccgacgt catcgaacgc gtggccaccg atctcgatgg tgcttcggtc
     3421 gaagtgttca gcgcgtcgcg ccggaaacgc ggcgtgatgc gcgacttccg aaaggtgctc
     3481 gagcgtctcg gtcagaacgg tgagctcatc tcgaaggcgc gcgagagtct cgtgagcctg
     3541 ggccggctgc tgaccttcgt acagcagagc actgcagtgc ccatgtcgca ggacgtgcgg
     3601 gcacgattcc gtactcagac ccgcgacgtc atggccatga gcgaccacgc ctcgttcctc
     3661 gggaccaagg tgtcgttcat cctcgacgcc acgctgggac tcatcaacat cgaccagaac
     3721 aacatcctca agatcttctc ggtcgtgacg gtgttcctgc tgccccctcg gtcatagggg
     3781 cggttttcgg catgaatttc tcgaaaatgc cctggctgca cgaaacctgg gggttctggg
     3841 cggccatggg cgtcatgctg gcatcggccc tcgggccttt cctcttcttc aagcggaaag
     3901 gctggctcta agaggctaga atccgcagcc cagcgcgccc gtagctcagc tggatagagt
     3961 gacggtttcc gaaaccgtag gtcacaggtt cgaatcctgt cgggcgcgcc agacttttat
     4021 acgacagata tacttcgcgc ccatgagcaa acaagactcc catttcttca ataatttcag
     4081 cttggtcctc ggcattctgg ttgccgtgac catcggcctg tttgcgttcg cgcggcacgt
     4141 cggcgccacg catcagggca agcaggtcac caccgatccg cggtatgtcc agagcgtcga
     4201 agaacgcctg cagcccgtat cccgggtggc cgttgccggc caggacaact cggcgctgaa
     4261 gattgaaagc gcgggcccgc ccgtggccgc cgccgcacta ccagcggacg gcaccgctgc
     4321 ctacgaaacc gcctgcgcgg cttgtcatgg tgccggcatc gccggcgccc ccaaggtcgg
     4381 cgacaagggc gcctggggac cacgcatggc tcagggcaag gaaactctct acaaccacgc
     4441 cattcacggc ttcaacggca agaccggtgt catgcctgcc aagggcggcc gcgccgactg
     4501 gccggatgac ctcatcaagc aggctgtcga tcacatgctg gagctgaacc agtaacgcgc
     4561 tcattcattc gcgcgccgca gctgcaaagc ttcggcgaga tgcacgggcc cgatggagcg
     4621 atcctgcggg cccgtttcat tatgcaggct gatcatttcc agatcggcga tggttcgcgc
     4681 cacgcgcaac acccggtgaa cacctcgggc ggacagcatg aaccggacgc gcgcctgccc
     4741 cagcaacagc cggcagcggc gatccagccg acacaggttc tcgatttcgc tgccgctcaa
     4801 gtcggcgttg agcttgccgg ttcggcggtg ctgcagctcg cgcgtattgc gaacgagccg
     4861 ggcagcgatg gcgctggtca acgcagcgtc agccggcgcg cgctcgccta acaactcttc
     4921 gctgggcaac gtgggaacct cgacgcgcag gtcgatgcga tcgagcagtg gcccggaaat
     4981 gcgggcgcga tagcgcgcga tttccggcgg tgcacagcgg cagcgcccgg aagtttctcc
     5041 gaggtaacca cagggacagg gattcatggc ggcgatcagc tgaaagcgcg ccggataatc
     5101 cacctggagt gccgcacggg aaatggccac cacgccggtt tcgagcggct ctcgcaggct
     5161 ttcgagcacg cgccgattga actcgggcag ttcatccagg aacaggactc cgcgatgcgc
     5221 gaggctcacc tcaccgggcc gcgcgtgcgg tccgccgccg atgatcgcgc tcgccgacgc
     5281 ggtgtgatgc ggcgcgcgga acggccgccg cccgtaatcg agcgggttga agccgcgagt
     5341 gctcaccgag gcgatggacg ccacatccag cgcttcgtcc gccgacagtg ttggcaacaa
     5401 acccggaagg cgctgcgcga gcatgctctt gccggtgcct ggcgggccga tcaacaggat
     5461 gctgtgcccg cccgccgccg cgacgagcag cgcgcgtttg ccggcggcct gaccgcgaac
     5521 gtcggccaga tcgggcgcaa cgcattcaac agatggcgcc gggtgacccg gcgccgcgag
     5581 ggcttcgaca ctgcctccgc cggccgccac cgggccgagc aatgcgcaca cgtctaacaa
     5641 ggttgccgca ccacgcgcag ccgcgggcgc gaccaggcac gcctcgccaa gattcccggc
     5701 aggcaccacc acgtggtgtt cgtcacgcgc cgcgtgcgcg gccgccagca acacgccctt
     5761 gatgggcttc agctcaccgg cgagcccgag ttcgccatag aactcggtgc gctcgagcga
     5821 ttcgtgccgc gcgtcgacct gttcggaggc cagaaggatg ccgagcgcga tcgggaggtc
     5881 gtagcggcag ccctccttgg gcaggtccgc cggagaaaga ttgaccgtga tgcgtccggc
     5941 cgggaattcg aaatcggagt tctgcagggc ggcgcggacg cggtccttgc tctctttcac
     6001 ggcggtggcc ggcaggccga cgatggagaa cacgggcagg cctgcgccca ggtgaacttc
     6061 gacctcgacc cgcggtgcgg ccaaccccag ctgcgcgcgg ctcgagacgc gtgcgattcc
     6121 catgaccact ccctggtcca atgcgtcgat aacgcgcgcc tgcatccatg ccggcggcat
     6181 ccttttctga actattaccg cccgctgttc gccctggctt cgagctcggc aactcgcttc
     6241 tcgagcgctt ccaggcgcgc ccgcgtgcgc tccagcacct ttcgttgagc atcgaattca
     6301 tcgcgcccgg ccagatcgag cttgccaagg ttggcgcgaa gcacggcccg gaaattgttt
     6361 tccagatctt cccgaagcgt gccggcaccg gatgcgagcc ctggcggcaa cccctgcatc
     6421 aggctacgta gcaattcttc gaccctgggt atctccactg gcggctctcc taacaagttg
     6481 acggcgttct gccctgtttt gtcgcgtgcc tgaatccacc gccccaaatt tgggcgcgat
     6541 ggcaggaacg ctgccttttt tcgcgccgca acggccctgt ctttgctgac agggggctgg
     6601 catggaacct gcactttgaa cacaggccct ggggccaact gatcgggagc atacgcgcgg
     6661 cgaagccgcg cgatccatcg gataacgatg ccaaagcgat gaaaatgatc actgcaatca
     6721 ttcgaccgtg gaagctcgac gaccttcaaa tggccgtcgc gcgcctcggc gtgcagggga
     6781 tcaccgtaac cgaggtcacg ggttacggcc ggcaacgggg gcacacggaa cacttccgtg
     6841 gggccgaata cagcatcgag ttcgtaccga agctccgggt cgagatcgtg ctcgataccg
     6901 cttccgccga agccgtgatc gaagccatta gcaatgtgtg tcgcaccgga aaaaccgggg
     6961 acggcaagat tttcctggcg ccagtcggtg aagcggtacg gatacgtacc cgtgaatccg
     7021 gcgcggcggc agcctgagat gtcgttgagg agcttttaca tgcatcaaga taagaagaga
     7081 acgaagggta gcagcctcat gcgcggtctc ggcgcagcgc tgctgatgat gttgccggcg
     7141 ctcg
//
LOCUS       DASQJS010000388         5505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_562278, whole genome shotgun sequence.
ACCESSION   DASQJS010000388 DASQJS010000000
VERSION     DASQJS010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562278"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VEW08_15265"
     CDS             <1..372
                     /locus_tag="VEW08_15265"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HYJ42151.1"
                     /translation="AYGRPGVLQGAYSLLLQDEDGQIQETDSVSAGLDYPGVGPEHSL
                     LLWAGRVRYEAATDDESLEALTECCRAEGILPAIESAHAFAGARRWAKENPGKKVLIG
                     LSGRGDKDMPTLQRTVLAGLK"
     gene            369..1145
                     /gene="trpA"
                     /locus_tag="VEW08_15270"
     CDS             369..1145
                     /gene="trpA"
                     /locus_tag="VEW08_15270"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HYJ42152.1"
                     /translation="MTPSERISAAIREARGGTAIVAFVTAGYPLREKFREHLTQVGSE
                     ADVVEIGVPFTDPMADGMTIQRSSREALRQGFSLRWLLDELQAMQGDGLLPRAPLILM
                     SYLNPLLAFGFDQLASAAVKAGVCGFIIPDLPYDEGEDLRAALAPHGLAIVQMVTPVT
                     QPERLKKLCAATQGFVYAVTMTGTTGRTASVPGEVTHYLDLVTAASKLPVCAGFGIRS
                     QQQVEQLRGHVAGVIVGSALVEVLENGQDPAAFLRSLRSS"
     gene            1152..2393
                     /locus_tag="VEW08_15275"
     CDS             1152..2393
                     /locus_tag="VEW08_15275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HYJ42153.1"
                     /translation="MKHQLPGYAMLAVLIGGLSMVSPFSIDTFFPAFHAMEKALNVDA
                     WKLQQVITAYMVPFAFASLVHGPLSDALGRRPVMIWGMALYTVGSVACAFAQNYEQLI
                     AARVLQGATAGVGVVIGRAIIRDLYEGAAAQRLMNLTTMIFGLAPAVAPIIGGWVHVA
                     FGWRAVFAVMVVCGVVFATASWWKLPETHPPHARIPFNARNLVATSVTVLRHPEFLMV
                     ALTAAINFCSLACFIGSAPAIIEKHWGMSETSYAALFLPVITGIMLGAVISRRIAGRM
                     DLTRQVHMGLSITFASALARVVLHLALDPLPIPLQQALLFVGAIGAQFAFPVLTLRML
                     DLFPAARGTAASAQSFVALLVTAFTLGIVSPKVLPHLEWIAWTSLTLTSIASLCWYFA
                     RRWQKPPVNPPDVPLHIPTEV"
     gene            complement(2529..>5505)
                     /locus_tag="VEW08_15280"
     CDS             complement(2529..>5505)
                     /locus_tag="VEW08_15280"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYJ42154.1"
                     /translation="RHHTLSFATAVAFFALASVVVFVAFVWINAARGERADSLRRAAE
                     RALRLSEERNQLIVETALDAVVTINHKGLVTGWNSHAEKLFGWPRAEALGRELAELII
                     PERLRDQHRAGMIRYVETGVARVLNKRIEMSAMHRDGTEFPVELAITPIGFGDDLVFS
                     GFIRDITGRVRAEAALRESEQRFRTTANAAPVLIWMSGKDKRCTWFNQRWLDFVGRDI
                     EQELGDGWCDNLHPADFDRALDTYHAAFDARRPYEMEFRLQRDDGAWRWLLERGAPHQ
                     GPQGEFVGFIGTCIDITEHRETVEQLRENRARFKTLAESLPQLIWTWLRDGYCDYLSR
                     QWLDYTGRSESQQMGHGWLEQVHPDDRVKVHEEWARVVASGDTYDISFRIRRFDGVYR
                     WFKTRAVPLRDPAGRILKWFGSNTDIEDFVVAERKLKVQLERMQLLDRTTHAIGAHQE
                     LRKVFEVVLRSLEDNLGIDFVTLCTYQAEPEVLTVSCVGERSAALAREIGVVEQARIE
                     VDENSLKRCVHGELVYEPDIENLEYPFTARLAGAGLRSLVAAPLMVESEVFGVMLIAK
                     RCVDAFTSDDCEFLRQLASHVALAAHQTRLYEALQGAYQDLRQTQQTVMQQERLRALG
                     QIASGIAHDINNALSPAALYAQSMLAHQTGLSERSREQLTVIQSAIDDVAQTVQRMRA
                     FYLPRGMENTLTPVDLNQVLRQVIDLTRARWSNIPQERGIVVHVESDLDADLPKILGA
                     ENEMRDGLTNLLLNAVDAMPDGGTITLRTRQVTRDHHVIVEVQDTGIGMSETTRSRCL
                     EPFFTTKGERGTGLGLPSVFGMVQRHGGDFEIDSELGRGTTMRMIFPRAPSGAAASEQ
                     QFVPPPRPLRLLLIDDDPLLLRSLRDALELDEHEVVTADGGQAGIDAFAHAVKSGPPF
                     DAVITDLGMPYVDGRKVAARVRQLSGQIPIIMLTGWGHRLIDTADQPEHVDRVLSKPP
                     KMAEIRGALTALVGPREAP"
BASE COUNT          866 a         1795 c         1815 g         1029 t
ORIGIN      
        1 gcctatggcc ggccgggcgt gctccagggc gcgtactcgc tgctgctgca ggacgaggac
       61 ggccagatcc aggaaaccga ttccgtttcc gcgggcctcg attatcccgg tgtcggtccc
      121 gagcattcgc tgctgctgtg ggccggacgc gtgcgctacg aagccgccac cgacgacgaa
      181 tcactcgaag cgctcaccga atgctgccgc gccgaaggca tcctgccggc catcgaatcg
      241 gcgcacgcat ttgcaggggc gcgtcgctgg gccaaggaga atcccggcaa gaaagtgctc
      301 atcgggctct cgggccgcgg tgacaaggac atgccgacat tgcaacgcac cgtgctggcg
      361 ggattgaaat gacaccttcc gaaagaatca gcgccgccat tcgtgaggcc aggggcggta
      421 ccgccatcgt ggcgttcgtc accgccggtt acccgttgcg cgagaaattc cgcgaacatc
      481 tcacccaggt cggcagcgaa gctgacgtcg tcgagatcgg cgtgccgttc accgatccga
      541 tggccgacgg catgaccatc cagcgttcga gccgcgaggc gctgcgccag ggtttcagcc
      601 tgcgctggct gctcgatgag ctgcaggcga tgcaaggtga tggtctgttg cctcgggcgc
      661 cgctgattct catgagttat ttgaatccat tgttggcatt cggtttcgac cagctcgcga
      721 gcgcggccgt gaaagccggc gtctgcggct tcatcattcc cgacctcccg tatgacgaag
      781 gcgaagacct gcgcgccgcg ctggcgccgc atggactggc catcgttcag atggtcacgc
      841 ccgtgactca gccagaacgc ctgaagaagc tgtgcgccgc gacgcagggg ttcgtatacg
      901 ccgtcacgat gaccggaacc accgggcgca cggcctcggt gcccggcgaa gtcacgcatt
      961 acctcgacct ggtgaccgcc gcatccaaac tacccgtgtg cgcgggattc ggcatccgca
     1021 gccagcaaca ggtggaacag ctccgcggcc acgtggccgg agtcatcgtc ggctcggcgc
     1081 tggtcgaggt actggaaaac ggccaggatc cggccgcatt cctgagatct ctgcgaagta
     1141 gttaaacctt ggtgaaacac cagcttccag gctacgcgat gctcgccgtg ctgatcggcg
     1201 ggctgtcgat ggtcagcccg ttctccatcg acacgttctt ccccgcgttc cacgcgatgg
     1261 agaaggcgct caatgtcgac gcgtggaaac tccagcaggt gatcacggcc tacatggtgc
     1321 cgttcgcatt cgcctcgctg gtgcacgggc cgctgtcgga tgcgctcggg cggcggccgg
     1381 tcatgatctg gggcatggcg ctgtacacgg tgggctcggt ggcctgcgcc ttcgcgcaga
     1441 actacgaaca gctgattgcc gcgcgcgtgt tgcagggtgc gaccgccggc gtgggcgtgg
     1501 tcatcggccg cgccatcatc cgcgatctgt acgaaggcgc cgcggcccag cgcctcatga
     1561 acctgaccac gatgatcttc gggctcgccc cggccgtggc gccgatcatc ggtggctggg
     1621 tgcacgtggc gttcggctgg cgcgcggtgt tcgcggtcat ggtggtctgc ggcgtggtct
     1681 tcgcgacggc gtcgtggtgg aaactgccgg agacgcatcc gccgcacgcg cgcatcccat
     1741 tcaatgcccg caaccttgta gcgacctcgg tcacggtgct gcgccatccg gaattcctga
     1801 tggtggcgct gaccgccgcg atcaacttct gctcgctcgc gtgtttcatc ggctccgcgc
     1861 ccgcgatcat cgagaaacac tgggggatga gcgagacttc gtatgccgcg ctgttcctgc
     1921 ccgtcatcac cggcatcatg ctgggtgccg tgatttcgcg ccgcatcgcc gggcgcatgg
     1981 acctgacccg ccaggtgcac atgggtctct cgatcacgtt cgcttcggcg ctcgcgcgcg
     2041 tggtcttgca cctggctctc gacccgctgc ccatcccgct gcaacaggcg ctgctgttcg
     2101 ttggcgccat cggcgcgcag ttcgcgttcc cggtgctcac gctgcgcatg ctcgatctgt
     2161 tccccgcggc tcgcggcacc gccgcctcgg cgcaatcatt cgtggctttg ctggtgaccg
     2221 cattcacgct cggcatcgtt tcgccaaagg tgctcccgca tctggagtgg attgcctgga
     2281 cgtcgctgac gctcacttcg atcgcgtcgc tgtgctggta cttcgcacgg cgctggcaga
     2341 agccgccggt caacccgccg gacgttccgc tgcacattcc caccgaagtc tagttctgcc
     2401 tcaggcaggg ttttccgaag cgcgtggaat atcgtcggac ggcgaacggc ctgcaggcgg
     2461 agattgccgg tcctggcagg gacgggaaag aagttgtggt gtccttcgag taccgcgcct
     2521 gcaactggtt acggcgcttc gcgcgggccc acgagcgccg tcagtgcacc ccgtatttcc
     2581 gccatcttcg ggggtttgct caacacccgg tcgacgtgct cgggctgatc ggcggtgtcg
     2641 atgaggcggt gtccccaacc cgtgagcatg atgatgggaa tctggccgga cagctgacgc
     2701 acgcgcgcgg cgacctttcg cccgtcgaca tagggcatgc cgagatcggt gatcaccgcg
     2761 tcgaatggtg gccccgactt cacggcgtgc gcgaacgcat cgatgcccgc ctggccgccg
     2821 tcggcagtga ccacctcgtg ttcgtcgagc tcgagcgcat cgcgcagcga gcggagcagc
     2881 aacggatcgt catcgatgag cagcaggcgc agcggccgcg gcggaggcac gaattgctgt
     2941 tcgctggccg cggcgccgga gggagcgcgc gggaaaatca tgcgcatggt ggtgccgcgc
     3001 ccaagctcgc tgtcgatctc gaagtcgccg ccatgacgtt gcaccatgcc gaacaccgat
     3061 ggcagcccga gccccgtgcc gcgctcgccc ttggtggtga aaaagggctc gagacagcgg
     3121 ctgcgcgtgg tttcactcat gccgatgccg gtgtcctgca cttcgactat cacgtggtga
     3181 tcgcgcgtca cctgtcgcgt gcgcagcgtg atcgtgccac cgtcgggcat cgcgtcgacc
     3241 gcgttcagca gcaggttggt cagcccgtca cgcatttcat tctccgcgcc gaggatcttc
     3301 ggcagatccg cgtcgagatc gctttcgacg tgcacgacaa tgccgcgttc ctgcgggatg
     3361 ttgctccagc gggcgcgcgt gaggtcgatc acctgcctga gcacctggtt gagatccacc
     3421 ggcgtcagcg tgttttccat gccgcgcggt agatagaacg cgcgcatgcg ctgcacggtc
     3481 tgcgccacgt cgtcgatggc gctctggatg acggtgagct gctcgcgcga tcgttcgctc
     3541 aggccggtct gatgcgcgag catggactgc gcatacagcg ccgccggcga cagcgcattg
     3601 ttgatgtcgt gggcgatgcc gctggcgatc tggccgagtg cacgcagccg ctcctgctgc
     3661 atgacggtct gctgggtctg gcggaggtcc tggtaagcgc cctgcagtgc ttcgtagagc
     3721 cgtgtctggt gcgcggccag cgccacgtgg ctggcgagct gccgcaggaa ctcgcagtcg
     3781 tcgctggtga atgcatcgac gcaccgcttc gcgatgagca tgacgccgaa cacctcgctc
     3841 tcgaccatca gcggcgctgc caccagcgag cgcagtccgg cacccgccag ccgtgcggtg
     3901 aacgggtact cgaggttctc gatgtcgggt tcgtacacca gttcgccgtg cacgcagcgt
     3961 ttcaggctgt tttcgtcgac ctcgatgcgg gcctgttcga ccacgccgat ttccctggcc
     4021 agcgccgcgc tgcgttcgcc cacgcagctg acggtgagca cctccggctc ggcctggtaa
     4081 gtgcagaggg tcacgaaatc gatgccgaga ttgtcctcga gactgcgcaa caccacttcg
     4141 aagaccttgc gcaattcctg gtgagcgcca atcgcatgcg tggtgcgatc gagaagctgc
     4201 atgcgttcga gctgcacttt cagcttgcgc tcggcgacga cgaaatcctc gatgtcggtg
     4261 ttcgaaccga accacttgag gatgcgaccg gccgggtcgc gcagcggaac ggcgcgggtc
     4321 ttgaaccagc ggtagacgcc gtcgaagcgg cgaatgcgga agctgatgtc ataggtgtcg
     4381 ccgctggcca cgacgcgggc ccactcctca tgcaccttga cccggtcgtc cggatgcacc
     4441 tgctcgagcc acccgtggcc catctgctgg gattcgctgc gcccggtgta atcgagccat
     4501 tgccggctca ggtaatcgca gtagccgtcg cgcagccagg tccagatcag ctgcggcagt
     4561 gattccgcca gtgtcttgaa tcgtgcgcga ttctcccgca gctgctccac ggtttcgcgg
     4621 tgctcggtga tgtcgatgca ggtgccgatg aaaccgacga actcgccctg tggtccctgg
     4681 tgtggcgcgc cgcgttccag cagccagcgc caggcgccgt cgtcgcgctg caggcggaac
     4741 tccatttcgt aggggcggcg cgcgtcgaat gccgcgtgat aggtgtcgag cgcacgatcg
     4801 aaatcggccg gatgcaggtt gtcgcaccag ccatcgccga gctcctgctc gatgtcgcgg
     4861 ccgacgaagt cgagccagcg ctggttgaac cacgtgcagc gcttgtcctt gccgctcatc
     4921 cagatgagca ccggtgccgc attcgccgtg gtgcggaaac gctgctcgct ttcgcgcagc
     4981 gcggcttcgg cacgcacgcg gccggtgatg tcacggatga acccgctgaa cacgaggtcg
     5041 tcgccgaacc cgatcggcgt gattgccagt tccacgggga actcggtacc gtcgcgatgc
     5101 attgccgaca tctcgatgcg cttgttcagc acccgggcga cgccggtttc cacgtagcga
     5161 atcatgccgg cgcgatgctg gtcgcgcagg cgctcgggga tgatgagctc ggcgagctcg
     5221 cggcccagcg cctcggcgcg tggccacccg aagagtttct cggcgtggga attccagccg
     5281 gtgacgagtc ccttgtggtt gatggtgacc acggcgtcga gcgcggtttc gacgatgagc
     5341 tggttccgct cctcggacag gcgcagcgcg cgttcggccg cgcgtctcag ggagtccgcg
     5401 cgttcgccac gcgccgcatt gatccagacg aaggcgacga aaaccacgac gctcgcgagc
     5461 gcaaaaaacg ccactgccgt cgcgaagctc aatgtgtgat gccgt
//
LOCUS       DASQJS010000389         4499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4648643, whole genome shotgun sequence.
ACCESSION   DASQJS010000389 DASQJS010000000
VERSION     DASQJS010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4648643"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VEW08_15285"
     CDS             <1..433
                     /locus_tag="VEW08_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012722612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYJ42155.1"
                     /translation="AEKFGMTHFVNPREVQGDLVPYLVSLTDGGADYSFECVGHVDLM
                     RQALECTHRGWGVSVIIGVAGAGQEIKTRPFQLVTGRVWKGTAFGGARGRTDVPKIVD
                     WYMDKKINIDDLITHVMPLDKINDAFDLMHKGESIRSVVTF"
     gene            461..1036
                     /gene="gfa"
                     /locus_tag="VEW08_15290"
     CDS             461..1036
                     /gene="gfa"
                     /locus_tag="VEW08_15290"
                     /EC_number="4.4.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051907 - S-(hydroxymethyl)glutathione
                     synthase activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione synthase"
                     /protein_id="HYJ42156.1"
                     /translation="MAIKLHPSIDKGLKPGAKKFKGGTLTCHCAADPVTVSIKGNVAH
                     NHACGCTKCWKPAGAKFSVVAVVSRDNLSVTANEQKLTTVDASAAIQRHACSGCGVHM
                     YGRIENTNHPFFGLDFVHVELSKDKGWAKPEFAAFVSSIIEAGAARPKEMTKIRDRLK
                     KLRLPPYDALSPPLMDAIATHVAKQKGTLKV"
     gene            1038..1877
                     /gene="fghA"
                     /locus_tag="VEW08_15295"
     CDS             1038..1877
                     /gene="fghA"
                     /locus_tag="VEW08_15295"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002050963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HYJ42157.1"
                     /translation="MLATISQHACFGGVLGYYQHPSRTNHCDMRFAVYAPPQAAAGPV
                     PVLYYLAGLTCTEETFVTKAGALEHAARHGIMLVAPDTSPRVQLPGDRESWDFGIAAG
                     FYLDATEAPWAAHYRMYSYVVDELPQVVAEYFKADVSRSGIMGHSMGGHGALTIALKN
                     PQKYKSLSAFAPIAAPIQSPWGNKAFTGYLGAYRASWSQYDATELVKAGARFAGGILI
                     DQGTSDKFLAEQLKPDLLVTACKEAGQECFLQMREGYDHGYFFIQSFISQHMTWHAHL
                     LAR"
     gene            complement(1902..2279)
                     /locus_tag="VEW08_15300"
     CDS             complement(1902..2279)
                     /locus_tag="VEW08_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYJ42158.1"
                     /translation="MNPDTTPPISGNVAGVAKDTRGWFLGHFMKGEDNPLRTSELELK
                     WYTHAKGETRNEWAPGNPVKTLNILIRGHFVLLFPDREIALAKEGDFVLFGPGVPHSF
                     RSEQESLVLTVRWPSLPPDKSSP"
     gene            complement(2276..3613)
                     /locus_tag="VEW08_15305"
     CDS             complement(2276..3613)
                     /locus_tag="VEW08_15305"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYJ42159.1"
                     /translation="MAGHRKLGPVLATIVVAGNMIGSGIFLLPATLATVGSLTVLGWV
                     IGAIGALALALLFARLAGRKPMAGGPATYAFDAFGRFWGSQSSLWYWAACLIGNVAIA
                     TAASSYLADFFGISAGPVQGAMFTLLLLWLVTLINLVSPRFVGQFDGVLLIAGLIPLL
                     LVVTIGWVAFDAAQFRESWNVSGQPLYKALPNSLALVFWAFTGLESASVAAAVVKNPE
                     RNVPIATIAGVLIAALIYIAASIVIFGLAPAADIAASNAPFALAAAKMMGPVAGPLVA
                     LCGGLKALGTLAGWVLMTAQVSRAAADHGLLPQTFARTRAGDTPVSGLIIAGLLGTAL
                     IILTIAPTLGKQFGYLAEASTLFALLTYLGACAAALRYRVRGELTLAVVGGAFCIFVI
                     AWSTLPVLIATLICLMLFALLYIPLSRRKYVLPDPTVRTGMQPTIRERFDDPT"
     gene            complement(3621..>4499)
                     /locus_tag="VEW08_15310"
     CDS             complement(3621..>4499)
                     /locus_tag="VEW08_15310"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYJ42160.1"
                     /translation="RRIVCPYHAWSYDFEGRLASLGDRAAFPHLDIGRESLAPVETEI
                     YAGFVFARLQPGGPSVAQMMAPYHDEIAPHEFEKLKPMGRVTLRPRKVNWKNVGDNYS
                     DALHIPVAHPGLTRLFGASYAVESREWIDRMSGELSNAASRNWAERMYQKYLPVAEHL
                     PPERRRTWVYFKMWPNFAFDVYPDQVDIMQWLPISPTECLIREIGYARPDDRREMRVA
                     RYCNWRINRQVNEEDRVLIERVQNGMASGSYRVGPLAAGEVALRSFGKRMRTLIPECA
                     QPATPRTGWSRPEARG"
BASE COUNT          887 a         1444 c         1408 g          760 t
ORIGIN      
        1 ggccgagaaa ttcggcatga ctcattttgt gaatccacgg gaggtgcagg gcgatctcgt
       61 tccgtacctc gtgtcgctca ccgacggcgg cgcggactac tctttcgagt gcgtgggcca
      121 cgtcgatctc atgcgccagg cgctcgagtg cacgcatcgc ggctggggcg tgtcggtcat
      181 cattggcgtc gcgggcgccg gccaggaaat caagacgcgg ccgttccagc tcgtgaccgg
      241 ccgtgtgtgg aaaggcaccg cgttcggcgg cgctcgcggc cgcaccgacg taccgaagat
      301 cgtcgactgg tacatggaca agaagatcaa catcgacgac ctcatcacgc acgtcatgcc
      361 gctcgacaag atcaacgacg ctttcgatct catgcacaag ggcgaatcca ttcgctccgt
      421 ggtcaccttc tgaacaacta acaacgacga gggaatgctc atggccatca agcttcatcc
      481 atcgatcgac aagggtctca aaccgggcgc caagaagttc aagggcggta cgctcacctg
      541 ccattgcgcc gcggaccccg tcaccgtctc catcaagggc aacgtggcgc acaaccacgc
      601 ctgcggctgc accaagtgct ggaaacccgc gggcgcgaag ttctccgtgg tcgcggtggt
      661 cagtcgcgac aatctcagcg ttaccgcgaa cgaacagaag ctgacgacgg tcgatgccag
      721 cgccgcgatc cagcgtcacg cctgcagtgg ctgcggtgtg cacatgtacg gacgcatcga
      781 gaacacgaac catccgttct tcgggctcga cttcgtgcac gtggagctgt cgaaagataa
      841 gggctgggcg aagccggagt tcgcggcgtt cgtgtcatcc atcatcgagg ccggcgccgc
      901 caggcccaag gagatgacca agatccgcga tcgcctcaag aagcttcgcc tgccgccgta
      961 cgacgcgctt tcgccgccac tcatggatgc gatcgccacg cacgtggcca aacagaaagg
     1021 cacgctcaag gtctgacatg ctcgcaacca tttcccagca cgcctgcttc gggggcgtgc
     1081 tgggttacta ccagcacccc tcgcggacca accactgcga catgcgtttt gcggtgtacg
     1141 cgccgccaca ggcggcggcg ggtcccgtac cggtgctgta ctacctcgct ggcctcacgt
     1201 gcaccgaaga aaccttcgtg accaaggccg gtgcgctcga gcacgcggcg cgacacggca
     1261 tcatgctggt ggcgccggac acgagcccgc gcgtccaact acccggcgat cgcgagtcct
     1321 gggatttcgg cattgccgcg ggtttttacc tggatgccac ggaagcgccg tgggcggctc
     1381 actaccgcat gtacagctac gtggtggacg agttgccgca agtggtcgcc gaatatttca
     1441 aggccgatgt ctcccgcagc ggcatcatgg gccactcgat gggcggtcac ggcgcgctga
     1501 ccatcgcgct gaaaaacccg cagaagtaca aatcactgtc ggcgttcgcg cccatcgccg
     1561 cgccgattca gagcccctgg ggcaacaagg cgtttactgg ttatctcggc gcgtatcgcg
     1621 cgtcgtggtc gcaatacgat gccaccgagc tcgtgaaagc gggtgcccgg ttcgccggtg
     1681 gaatcctcat cgaccagggc acctccgaca agtttcttgc cgagcagctc aagcctgatt
     1741 tgctggtgac ggcgtgcaag gaagcggggc aggaatgctt cctgcagatg cgcgagggtt
     1801 acgaccacgg ctacttcttc atccagagct tcatctcgca acacatgacc tggcacgcgc
     1861 acctgctcgc caggtagcag aagctcagtc gtcgaagcgt ttcagggcga cgacttgtcg
     1921 ggcggcagcg aaggccagcg taccgtcagc acaagagatt cctgctccga tcgaaacgag
     1981 tgcggcacgc ctggaccaaa cagcacaaag tcaccttcct tcgccagggc gatttcccga
     2041 tccggaaaga gcaggacgaa gtgaccgcga ataaggatgt tgagcgtctt caccgggttg
     2101 ccgggcgccc actcattgcg tgtctcaccc ttcgcatgcg tgtaccactt caactccagc
     2161 tcggatgttc gaagcgggtt gtcctcgccc ttcatgaagt gtccaagaaa ccagccgcgc
     2221 gtgtccttgg cgacgccggc aacgtttccg gaaattggag gagtcgtatc cgggttcatg
     2281 tcgggtcgtc gaagcgttcg cgaattgtcg gttgcatgcc tgtgcgcact gtagggtcgg
     2341 gcagcacata tttgcgtcgc gacagcggga tgtacagcag cgcgaacagc atcaggcaga
     2401 tgagcgtggc gatgagcacc ggcagcgtcg accacgcgat cacgaatatg cagaacgcgc
     2461 cgccgaccac cgcgagcgtg agttcaccgc gcacccgata acgcagtgcc gccgcgcagg
     2521 cgcccaggta ggtgagcagc gcgaacagtg tcgaggcctc agccaggtac ccgaactgct
     2581 tgcccagcgt gggggcgatg gtcagtatga tgagagcggt ccctaacaag ccggcgatga
     2641 tgagtccgct caccggggtg tcgcccgcac gcgtacgtgc gaacgtctgc ggcagcaggc
     2701 catgatcggc ggcggcgcgg ctcacctgcg ccgtcatgag tacccaaccc gcgagtgtgc
     2761 ccagcgcctt caagccgccg cacagcgcca ccagcggtcc ggcgaccggt cccatcattt
     2821 tcgcggccgc cagggcaaaa ggtgcgttgg aagcggcaat atcggcagcc ggcgccaggc
     2881 cgaagattac gatgctggct gctatatata tgagtgccgc aatgagcaca cccgcgatcg
     2941 tggcgatggg cacattacgt tccggattct tgactaccgc cgccgccacc gaggcgctct
     3001 cgagaccggt gaaagcccag aacaccaggg ccagtgaatt cggcagcgcc ttgtacaagg
     3061 gttggccact gacattccac gactcgcgga actgcgcggc atcgaaggcc acccagccaa
     3121 tcgtgaccac cagcagcagg ggaatgagcc cggcgatgag caacactcca tcgaactggc
     3181 ctacgaagcg cgggctcacc aggttgatca acgtgacgag ccacaacagc agcaaggtga
     3241 acatcgcccc ttgcaccgga cccgcgctga tgccgaagaa gtcggcgaga taactcgatg
     3301 ccgccgtggc aatggccacg ttgccgatca ggcatgccgc ccaataccag aggctggact
     3361 gcgaacccca gaatctgccg aacgcatcga atgcataggt ggcgggtccg cccgccatcg
     3421 gcttgcgacc ggccagccgc gcaaacagaa gtgccagtgc cagggcgcca atcgcgccga
     3481 tgacccagcc gagcacggtg aggctgccaa ccgtggcgag cgtggccggc aacaggaaga
     3541 ttcccgagcc gatcatgttg ccggcaacga caatcgtggc gagtaccggc cctaacttgc
     3601 ggtggccggc cactaactat tcatccccga gcctccgggc gcgaccagcc tgtgcggggt
     3661 gtcgcgggtt gcgcgcactc cggaatcagc gtgcgcatgc gtttgccgaa gctgcgcaag
     3721 gcgacctcgc cggcggcgag ggggcccacg cggtagctgc ccgaagccat gccgttctgc
     3781 acgcgctcga tgagtacccg gtcttcctcg ttcacctgcc ggttgatgcg ccagttgcaa
     3841 tagcgggcca cgcgcatctc gcgccggtcg tcgggccgcg catacccgat ctcgcggatc
     3901 aggcattcgg tgggcgatat cggcagccac tgcatgatgt ccacctggtc gggatagacg
     3961 tcgaaagcga aattgggcca catcttgaaa tacacccacg tgcggcggcg ctcgggtggc
     4021 aggtgctcgg caaccggcag gtatttctgg tacatgcgct ccgcccagtt gcgcgaggcc
     4081 gcattgctga gctcgcccga catgcgatct atccactcgc gcgattcgac ggcgtagctc
     4141 gcgccaaaca acctcgtgag cccggggtgc gccaccggga tgtgcaacgc gtcggaatag
     4201 ttgtcgccga cgtttttcca gttgaccttg cggggccgca gcgtgacgcg gcccatgggt
     4261 ttcagctttt cgaactcgtg cggggcgatc tcatcgtgat atggcgccat catctgcgcc
     4321 acggatggac cgcccggctg cagccgggcg aacacgaacc cggcatagat ctcggtttcc
     4381 accggcgcca gcgattcgcg gccgatgtcg aggtgcggaa aagccgcccg atcgccgagc
     4441 gaagccagcc ggccttcgaa gtcgtaagac catgcgtggt agggacacac gatgcggcg
//
LOCUS       DASQJS010000390         5431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2454106, whole genome shotgun sequence.
ACCESSION   DASQJS010000390 DASQJS010000000
VERSION     DASQJS010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2454106"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /gene="arfB"
                     /locus_tag="VEW08_15315"
     CDS             complement(<1..418)
                     /gene="arfB"
                     /locus_tag="VEW08_15315"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HYJ42161.1"
                     /translation="MSLVVRPGLEIPDSDLEVAFIRSAGPGGQNVNKVASAVQLRFSV
                     ARNTTLRDDVKARLRALAGQRLTDADEILIQARESRSQESNRRDAEERLLDLVRRALV
                     VPKKRHATKPTRASKERRLDGKARSQKNKRLRGKVKF"
     gene            complement(415..1692)
                     /locus_tag="VEW08_15320"
     CDS             complement(415..1692)
                     /locus_tag="VEW08_15320"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HYJ42162.1"
                     /translation="MKKLFEQLTTPEVMAEALALLMAALLATALAHYIKNWLRPIAAR
                     DNLPRWTARIVVAGMVLAPSLLGLLLILGLRVLFAGFRLETALIDVAMDLATVLVLVR
                     IAVHVLSVSLGPNSWIRAWELRITFALWAAISFQVLGWFSGIERTFDGIDLVPGKTTF
                     TLWALLKSIVVIVGFLVATSLIARTIERRVMKLEGLAISTRIGISKFSTFFLVGLGVL
                     LGINASGVDLTVLGLFTGAIGLGLGFGLQAIASNFVSGFVLLLDKSIKPGDVISFTGT
                     TGTSTENFGWVQELRGRYIVVRDRDGVETLVPNQNLITNSVINWSYSDQRVRIRLPVM
                     ISFEDDPEVALQVLIDAAANHPRILKEPGPVSRLMSFENYGMRVEVRFWIRDPMNGVN
                     NVRSDVNREIWRLFKKHGITIPVQQHDVRVIQG"
     gene            complement(1689..1919)
                     /locus_tag="VEW08_15325"
     CDS             complement(1689..1919)
                     /locus_tag="VEW08_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HYJ42163.1"
                     /translation="MSEIEDQDNVDRELFKNVEKLRELRIAHRDLDEVISRLSLDFQT
                     DQLQLKRLKKRKLILKDQITRLESELIPDLNA"
     gene            complement(1916..2773)
                     /locus_tag="VEW08_15330"
     CDS             complement(1916..2773)
                     /locus_tag="VEW08_15330"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HYJ42164.1"
                     /translation="MDATPETLVDFHTHSHYSDGVLSPTALVERARTRRVGTLALTDH
                     DTTAGLAEARTACAASQIHFVPGAELSAQWRGQTIHIVGLQVDESHAGFNSHLDAVLQ
                     RRQERLREIGERLEKRARLPGRELAALAAEATAPTRLHLARLLVERGHARDTQEAFDR
                     WLNRDKAGHVPAEWPALAAAMAVLRDSGAIAVLAHPHRYRLSAGQLRELTAAFALEGG
                     AALEASMAGMSPNDADRIASLCRRFKLRASMGSDFHDPAVPWNPLGRWLKLASGLDPV
                     TTCLTEHAR"
     gene            2798..3478
                     /gene="yrfG"
                     /locus_tag="VEW08_15335"
     CDS             2798..3478
                     /gene="yrfG"
                     /locus_tag="VEW08_15335"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF011564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HYJ42165.1"
                     /translation="MSRHPHSRPDWSAIDTVLLDLDGTLLDQAYDNHIWRDLVPQRFA
                     VAQGLDLHVAYSEIARRFAERNGTLDWYCIDYWSRSLGIDIGALHREVRSHVAWLPGA
                     REFLMRMRAAGKKLVLLTNSHPTALAVKHEETGVLDYLDSAATSHEFGAPKENEQFWR
                     AAVARFAFDPARSLFADDNSKMLEAARAAGIRWVYGVRHWDTKGSRRDHVDHFAVDGV
                     GDLAEFSN"
     gene            complement(3525..4892)
                     /locus_tag="VEW08_15340"
     CDS             complement(3525..4892)
                     /locus_tag="VEW08_15340"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYJ42166.1"
                     /translation="MTETHLSELAFASLDLVPSVAQGIRDAGFVRATPVQAMTLPIAL
                     TGKDVAGQAQTGTGKTAAFLVALFQRLLTNPMPGRSASGIRALILAPTRELAVQIFSD
                     AEVLGKHTGLKIGVVYGGVDYEKQRKSLQDGIDVLIGTPGRVIDYFKQHVFDMRHAQA
                     VVLDEADRMFDLGFIADIRFILRRLPKPELRQSMLFSATLSQRVLELAYEHMNNPELV
                     RIEPDRITADKVRQMIYFPAMEEKIPLLIGLLRQYEARRTMVFVNTKRVAEKLEAYLI
                     ANGFKAQALSGDVPQKKRLRFMKEFHSGELAVLICTDVASRGLHIADVSHVFNYDLPQ
                     DAADYVHRIGRTARAGAEGDAISFGCEEFAVSLPEIEAYITHKIPVFPVKLELLAKDL
                     KPPVYKEWDPEDGPKPMRRSGGGGGGGRGGPRRGGPGGGRGGPPRRSGGGGGGGRGGP
                     RRGGR"
     gene            5220..>5431
                     /locus_tag="VEW08_15345"
     CDS             5220..>5431
                     /locus_tag="VEW08_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HYJ42167.1"
                     /translation="MSNPDIVHTTDATFSQDVLKSDKPVLLDFWAEWCGPCKMIAPIL
                     DEIATEYKDKLRVAKIDIESNPQTPP"
BASE COUNT          991 a         1714 c         1743 g          983 t
ORIGIN      
        1 cgaatttgac cttgccacgt aggcgcttat ttttctgcga gcgcgccttt ccgtccagtc
       61 gacgctcttt cgaggcgcgt gtgggttttg tcgcatggcg cttcttgggg actaccaatg
      121 cgcgacggac gagatcgagg agccgttctt ccgcgtcgcg tcgattgctt tcctggctgc
      181 gcgactcgcg cgcctggatg aggatctcat ccgcgtcggt gagtcgctgg ccggcgagcg
      241 cgcgtaatcg cgccttcacg tcatcgcgca gggtcgtatt acgcgccacc gaaaatcgca
      301 gctgtacggc cgacgccacc ttgttgacgt tctgcccgcc gggcccggcc gagcggatga
      361 acgccacttc cagatcggag tcggggatct cgagccccgg tcgaacgacc agactcatcc
      421 ctggatcacg cgcacgtcgt gctgctgcac ggggatggtg atgccgtgtt tcttgaacag
      481 gcgccagatc tcgcgattga cgtccgaacg cacgttgttg acgccgttca tgggatcacg
      541 gatccagaag cgcacttcga cgcgcatgcc gtagttctcg aagctcatga ggcgcgagac
      601 cgggccgggt tccttgagga ttcgcggatg attggctgcg gcgtcgatga gcacctgcaa
      661 ggcgacttcc ggatcgtcct cgaaactgat catgaccggc agccggatgc gcacgcgctg
      721 atcggagtag ctccagttga tcaccgagtt ggtgatgaga ttctggttgg gcaccagcgt
      781 ttcgacgccg tcccggtcgc gcacgacgat gtagcggccg cgcagctcct gcacccagcc
      841 gaagttttcg gtgctggtgc cggtggtgcc ggtgaaactg atgacgtcgc cgggcttgat
      901 ggacttgtcg agcagcagca cgaagccgga cacgaagttg ctggcgatgg cctgaaggcc
      961 gaagcccaat cccaggccga tggcgccggt gaacagcccc agcaccgtga gatcgacgcc
     1021 cgaggcgttg atgcctaaca acacaccgag gccgaccagg aagaacgtgg agaacttcga
     1081 gatgccgatg cgcgtcgaga tcgcgaggcc ctcgagcttc atgacgcgcc gctcgatggt
     1141 gcgcgcgatg aggctcgtgg cgaccaggaa tccgacgatg accacgatgc tcttgagcag
     1201 cgcccagagg gtgaaggtgg tcttgccggg gacgaggtcg atgccgtcga acgtgcgctc
     1261 gatgccgctg aaccagccca gaacctggaa cgagattgcc gcccagagcg cgaaggtgat
     1321 gcgcagttcc caggcgcgga tccagctgtt cggccccagc gaaacgctca ggacatgaac
     1381 cgcgatgcgc accagcacca gtacggtggc caggtccatg gctacgtcga tcagcgcggt
     1441 ttccagccgg aagccggcga acagcacacg cagccccagg atgagcagca ggcccaggag
     1501 cgatggcgcc agtaccatgc ccgcgaccac gatgcgcgca gtccagcgcg gcaggttgtc
     1561 gcgcgcggcg atcggacgca gccagttctt gatgtagtgc gccagcgccg tggccagcaa
     1621 cgcggccatg agaagcgcga gcgcctcagc catgacttcg ggggtggtca gttgctcgaa
     1681 gagtttcttc aagcgttcag atccgggatg agctcactct cgagccgggt gatctggtcc
     1741 ttgagaatca gcttgcgctt cttaagccgc ttcagttgga gctggtcggt ctggaagtcg
     1801 agcgacagcc gcgaaatgac ctcgtcgaga tcgcgatgcg cgatgcgcag ttcgcgcagc
     1861 ttttccacat tcttgaaaag ctcgcgatcg acgttgtcct gatcttcgat ttcgctcatc
     1921 gggcgtgttc agtaaggcaa gtcgtcaccg gatcgagacc gctcgcaagc ttaagccacc
     1981 ggccgagggg attccacggc acggcggggt cgtggaagtc cgaacccatc gaggcacgca
     2041 gcttgaagcg gcggcacaac gatgcgatgc ggtcggcgtc gttcggactc atgccggcca
     2101 tgctggcttc gagcgccgcg ccgccctcca gcgcgaaagc cgcggtcagc tcgcgcaact
     2161 ggccggcgga caggcgatag cgatgcggat gagcaagcac ggcaatggcg ccgctgtcgc
     2221 gcaataccgc catggccgcc gcgagggccg gccattcggc gggaacgtgg cccgccttgt
     2281 cgcgattcag ccaacggtcg aacgcctcct gggtgtcgcg cgcgtgcccg cgttccacga
     2341 gcaggcgcgc caggtgcagc cgcgtgggtg ccgttgcttc cgcggcaagg gcggcgagct
     2401 cgcggcccgg cagacgcgcg cgtttctcga gccgctcgcc gatttccctt aggcgctcct
     2461 ggcgtcgctg caggaccgca tcgagatgcg aattgaagcc cgcatgggac tcgtcgacct
     2521 gcaagccgac gatgtgaata gtttggccgc gccactgcgc cgagagttcg gcaccaggca
     2581 cgaagtggat ctgcgaagcc gcgcaggcgg tgcgcgcctc ggcgagcccc gcggtggtgt
     2641 cgtgatcggt gagcgccagt gtgccgacgc gacgggttcg tgcgcgctcg accagcgcgg
     2701 tgggcgacag cacaccatcc gaatagtggc tgtgcgtgtg gaagtcgacg agggtttcgg
     2761 gggtggcgtc catcgggggc cagtatgata cgcccgtatg tcccgccacc cgcattcccg
     2821 gcccgattgg tcggccatag ataccgtgtt gctcgacctc gacggcacgc tgctcgacca
     2881 ggcgtatgac aaccacatct ggcgcgacct cgtgccgcag cgcttcgccg tggcgcaggg
     2941 cctcgatctg cacgtggcgt actcggaaat tgcgcgccgt ttcgcggagc gcaacgggac
     3001 gctggactgg tattgcatcg actactggtc gcgctcactc gggatcgaca tcggtgcact
     3061 gcatcgcgaa gtgcgttcgc atgtcgcctg gttgccgggc gcccgcgaat tcctcatgcg
     3121 catgcgtgcg gcggggaaaa aactggtgct gctcaccaat tcgcatccca ccgcgctcgc
     3181 cgtgaaacac gaagagaccg gcgtgctcga ctatctcgac tcggccgcca cgtcacacga
     3241 attcggggcg cccaaggaaa acgaacagtt ctggcgcgcc gcggttgccc ggtttgcctt
     3301 cgatcctgcc cgcagcctgt tcgccgacga caactcgaag atgctcgaag ccgcgcgcgc
     3361 cgcgggcatt cgctgggtgt acggcgtgcg ccattgggac accaagggct cgcggcgcga
     3421 ccatgtcgac cattttgcgg tcgatggtgt cggagacctg gcggagttct ctaactagac
     3481 cggaaatggg gacattcccc atttgcggca aatgagggac gtccctagcg accgccgcgg
     3541 cgcgggccgc cgcgtccacc accaccacca ccaccactgc gccggggcgg gccgccacgt
     3601 ccaccgccag ggccgccgcg acgcggacca ccgcgtccac cgccaccgcc gccgccggag
     3661 cggcgcatgg gcttggggcc atcttccggg tcccattcct tgtagaccgg cggcttcagg
     3721 tccttggcta acaactcgag cttcaccggg aacacgggaa tcttgtgcgt gatgtaggcc
     3781 tcgatctccg gcaacgacac tgcaaattcc tcgcagccga agctgatcgc gtcaccctcg
     3841 gcgcccgcgc gcgcggtgcg gccgatgcgg tgtacgtaat cggcggcatc ctgcggcagg
     3901 tcgtaattga aaacgtggct gacatccgcg atgtgcaggc cgcgcgaggc aacgtccgtg
     3961 cagatcagca ccgcaagctc gccgctgtga aactctttca tgaaacgcag gcgcttcttc
     4021 tgcggcacgt cgcccgacag cgcctgcgcc ttgaagccgt tggcgatgag gtaggcctcg
     4081 agtttctccg cgacgcgctt ggtgttcacg aaaaccatcg tgcggcgcgc ctcgtactgc
     4141 cgcagcaggc cgatgagcaa cgggattttt tcttccatcg ccgggaaata gatcatctgc
     4201 cgcactttgt ccgcggtgat gcgatccggc tcgatacgca cgagctccgg attgttcatg
     4261 tgttcatagg cgagctcgag cacacgctgc gacagcgtgg ccgagaacaa catcgactgc
     4321 cgcagctcgg gcttcggcag ccggcgcaga atgaagcgga tatcggcgat gaaccccagg
     4381 tcgaacatgc gatcggcttc gtccagcacc acggcctgcg cgtggcgcat gtcgaacacg
     4441 tgttgcttga aatagtcgat gacacggccg ggcgtgccga tcagcacgtc gatgccgtcc
     4501 tggaggctct tgcgttgttt ttcgtagtcg acgcccccat aaaccacgcc gatcttcaat
     4561 ccggtgtgtt tacccaacac ctcggcgtca ctgaaaatct gcaccgccag ctcgcgcgtg
     4621 ggcgcgagga tcagggcgcg gatgccgctg gcgcttcgcc ccggcatggg gttcgtgagc
     4681 aggcgctgga acagcgccac caggaaggcg gctgttttgc cggtaccggt ctgggcttgt
     4741 cccgcgacat cctttcccgt gagggcgatt ggcaacgtca ttgcctgaac cggggtggcg
     4801 cggacgaaac cggcgtcgcg gatgccctgg gcgaccgagg gaacgagatc gagcgaagcg
     4861 aaagcaagct cggaaaggtg tgtctctgtc attgaattga tttacttatg tattggggac
     4921 atcgaatagg cgccgggtgc atgtggcgct tgcaaatggc agtcggacca gttaaaaaat
     4981 gctcccaaga cgcggacctg ctcgtgtatc gagctcaccg aaccgcaaat ccaaagcttc
     5041 caaaccaaac actgggtctt ttaatccatc aggcaatttg actgcgcaac ggcagtctaa
     5101 ccgtttgatc gcgtcctgag cacggcttcc tgccggctgc agcgccagct cctcaaaacc
     5161 ccaccaatac accactcgag tctcaatgca tttctgtttc cctatggagg tccttccacg
     5221 tgagtaaccc ggacatcgtc cacactacgg acgcaacgtt cagccaggac gtgctcaagt
     5281 ccgacaagcc cgttcttctc gacttctggg ccgagtggtg tgggccctgc aagatgatcg
     5341 cgccgatcct cgatgagatc gccacggagt acaaagacaa gctccgcgtg gccaagatcg
     5401 acatcgagtc gaatccgcag acgccgccga a
//
LOCUS       DASQJS010000391         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3705512, whole genome shotgun sequence.
ACCESSION   DASQJS010000391 DASQJS010000000
VERSION     DASQJS010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3705512"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2406)
                     /locus_tag="VEW08_15350"
     CDS             complement(<1..2406)
                     /locus_tag="VEW08_15350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYJ42168.1"
                     /translation="MDQTPESARDTFVSFAIWMQNLRQTAYASPVLVLSVEGQAVGEW
                     DSRNDSMLRDQERYARAMSTSNDGFWDWIVADDAIYTSPKLLEIYGFAPGTSFAGRED
                     LIARIPFQSEDRQMVARVFAEHFAGKTARCDVEARFFRGGELRWVQLGGVAARDSFGA
                     VVRWTGTVRDITERKRAEDALRETQERYARVVSASEEAFWDWNAPVDELYLSPRFLDM
                     FGIPPDTTFARRADMFKALPISDVDREALQRAAAEHFAGKTARMEIEIRASVRGAEKW
                     VHFIGRATHNSEGQLVRWNGTLRDVTARKRAEQALRESQDRYELAMAASESGYWDWHI
                     PTNQYFLSSRALEMGGYDPDTGISRDEFRSRINMHPEDFARWEAAREALFAGAGERLA
                     MEVRYIVRGEIRWHSLQAICKRDDTGKVIRWTGSTTDITDRKRAEDELKTMERKLRLS
                     QRLEAMGTLAGGIAHDFNNILGAVLGYGEMAMRDAPKGSRLARDLDAIMIAGERGRAL
                     VDRVLAFSRSSVGERVPVNVERVVREVLDQVQAKLPPDVTVHAKMHAERAAVRGDATQ
                     VHQVVTNLANNAVQAMPAGGTLRIWLAVERAEVPRTATIGIREAGEYVVLTVADTGTG
                     IPPEIADRIFDPFFTTKDVGTGTGLGLSLVHGIVTELGGAIDVASTPGAGSTFTIYLP
                     RCGDAAESVDAREADLPRGRGQRVLIVDDEEPLVQLASRTLEELGYAPVGFTSSSAAL
                     AALRAGPQHFDALITDERMPEMSGSELIREVRGMCGPMPIVLMSGYLGTALGEADEVL
                     QKPL"
     gene            2514..>2984
                     /locus_tag="VEW08_15355"
     CDS             2514..>2984
                     /locus_tag="VEW08_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYJ42169.1"
                     /translation="METQRNSGYLHLVGADLPIDSFRVAAGNTARLPPHQLQLAVADI
                     SRALNEALRKNGINAMLYLRRADESLTPEARPELSRTAIAGPRLTAWQQRRVLEHIES
                     GLATPLRNRELAALVRFSEFHFNVAFRNSLGTSPHEFIIRRRIDRAQQLMLSTRM"
BASE COUNT          528 a          990 c          925 g          541 t
ORIGIN      
        1 aagcggtttc tgcaagacct cgtcggcctc acccagtgcc gtgcccagat agccgctcat
       61 cagaacgatg ggcatgggcc cgcacatgcc gcgcacttca cgaatcagct cggagcccga
      121 catctccggc atgcgctcat cggtgatcag cgcatcgaag tgctgcgggc ctgcgcggag
      181 cgccgcgagg gcggcgctgc tggaggtgaa cccgaccggc gcgtagccca gttcctcgag
      241 cgtgcgcgag gccagctgca ccagcggctc ctcgtcgtca acgatcagca cgcgttgccc
      301 cctgccgcgc ggaagatccg cttccctcgc gtcgacagac tcggcggcat cgccgcagcg
      361 cggcagatag atggtgaacg tgctgccggc gccgggtgtg ctcgcgacgt cgatggcgcc
      421 accgagttcg gtgacgatgc cgtgcaccag cgagagaccg aggcccgttc cggtgccaac
      481 atccttggtg gtgaaaaagg ggtcgaaaat ccgatcggcg atttccggcg ggataccggt
      541 gccggtatcc gccaccgtca acacgacata ctcgccggcc tcgcgaatgc cgatcgttgc
      601 ggtgcgtggc acttcggcgc gctccacggc gagccagatg cgcagcgtcc ctccagccgg
      661 catcgcctgg acggcgttgt tcgccagatt ggtcaccacc tggtgcacct gtgtcgcgtc
      721 gccgcgtacg gcggctcgct cggcgtgcat cttggcgtgc acggtgacat cgggcggcag
      781 cttcgcctgg acctggtcga gcacctcgcg caccacgcgt tccacgttga cgggcacacg
      841 ttcgcccacc gagctgcggc tgaacgcgag cacgcggtcg acgagcgctc gcccgcgctc
      901 gccggcgatc atgatggcgt cgaggtcgcg cgcgaggcga ctgcccttcg gcgcgtcgcg
      961 catcgccatt tcgccgtatc cgaggactgc gccgaggatg ttgttgaagt cgtgcgcgat
     1021 gccacccgcg agtgtcccca tggcctcgag gcgttgagac aggcgcaact tccgctccat
     1081 ggtcttcaac tcatcttcag cccgcttgcg gtccgtgatg tcggtcgttg agccagtcca
     1141 ccggatgacc ttgcccgtgt cgtcgcgttt gcagatggcc tgaagactat gccagcggat
     1201 ctctccgcga acgatgtaac gcacctccat ggcaagccgt tcgcccgcgc cggcgaacaa
     1261 cgcttcgcgc gccgcctccc atcgcgcgaa atcttccggg tgcatgttga ttcgcgaacg
     1321 gaactcatcc ctggagattc cggtgtccgg atcgtagcct cccatctcga gcgctctcga
     1381 ggagaggaag tactggttgg tgggaatgtg ccaatcccag tatcccgact cgctcgcagc
     1441 catggcgagt tcgtagcggt cctgggattc gcgcagcgcc tgttcagccc gcttgcgcgc
     1501 ggtcacatcc cggagggtgc cgttccagcg taccaactgc ccctcggaat tgtgcgtcgc
     1561 gcggccgata aaatggaccc acttctccgc gccgcgcacc gaggcgcgaa tctcgatctc
     1621 catgcgcgcg gtctttccgg cgaagtgttc ggcggctgca cgctgcaagg cctcgcggtc
     1681 gacgtccgag atcggcaggg ctttgaacat gtcagcgcgc cgcgcaaacg tcgtgtccgg
     1741 tgggatgccg aacatgtcga ggaaccgcgg cgagagataa agctcatcga ccggtgcatt
     1801 ccaatcccag aacgcctcct cgctcgcgct caccacgcgc gcataacgct cctgggtttc
     1861 gcgcagcgca tcttcggcgc gcttgcgctc ggtgatgtcg cgcaccgtgc cagtccacct
     1921 gactacagcg ccgaaagagt cgcgcgctgc cacgccgcca agctgcaccc aacgcagctc
     1981 gccgccccgg aagaagcgag cctcgacgtc gcagcgtgcc gtcttgccgg caaaatgttc
     2041 ggcaaagacc cgagccacca tctggcggtc ctcggactgg aatggaatcc gggcgatgag
     2101 gtcttcgcgg ccggcgaaac tggtgcctgg cgcgaagcca tagatctcca gaagcttcgg
     2161 cgacgtgtag atagcatcgt cggcgacgat ccagtcccag aaaccatcat tactggtgct
     2221 catggcacgt gcgtaccgct cctggtcgcg caacatgctg tcatttcgag agtcccactc
     2281 acccacggct tgtccctcta ctgacaagac gagaaccggc gaagcatacg ccgtctgtcg
     2341 caggttctgc atccagatgg caaatgacac aaaggtgtcg cgcgccgact caggcgtttg
     2401 gtccatgtca caggcgtcat ctcaggaaca ggtcttgcaa acaacctgcg acttgcgggg
     2461 agtcgaatgt catcccatga ggagccggcg cggctcaccc tgggagacag gtgatggaaa
     2521 ctcaacgcaa ttcaggctat ttgcacctcg ttggagcaga ccttccgatt gattcgttcc
     2581 gggtggcggc cggcaatacg gcgcggctgc cgccgcacca gctgcagtta gccgtagcgg
     2641 atatatcgag ggcgctgaat gaggcgcttc gcaagaacgg catcaatgca atgctgtatc
     2701 tgcgacgcgc ggatgagtcc ctgacgcctg aggcgcggcc ggaattatcg cgcaccgcga
     2761 ttgccggtcc gaggctgacc gcctggcaac agcggcgggt gctggaacac atcgaatccg
     2821 gcctcgcaac gccattgaga aaccgcgaac tcgcggccct ggtgcgtttc agcgagtttc
     2881 atttcaatgt cgcattccgc aacagtctgg gcacttcgcc tcatgaattc atcattcggc
     2941 ggcgaatcga tcgcgcgcag cagctcatgc tctccaccag gatg
//
LOCUS       DASQJS010000392        10744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4028282, whole genome shotgun sequence.
ACCESSION   DASQJS010000392 DASQJS010000000
VERSION     DASQJS010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10744
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4028282"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..555
                     /locus_tag="VEW08_15360"
     CDS             25..555
                     /locus_tag="VEW08_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42170.1"
                     /translation="MGPRSSPADVLRVGKEAHASVVGLTHRFGVELRQLAAGEILPGS
                     FWGESEAGLRANVLYVRADTPLHSLLHELSHYVCMSPERRASLDRDAGGDDEEECAVC
                     YLQILLADELPMLGRDRMLEDMDAWGYTFRLGSSRAWFEQDAADACTWLRGHGIVDDG
                     NRPTFRLKGAALSRGG"
     gene            complement(774..1790)
                     /gene="pip"
                     /locus_tag="VEW08_15365"
     CDS             complement(774..1790)
                     /gene="pip"
                     /locus_tag="VEW08_15365"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HYJ42171.1"
                     /translation="MKSKAAKSKKRAPKAAAPARKAGDFYPAIKAYNTGFLRVSDVHE
                     LYYEESGNPNGKPVVFLHGGPGGGTDPKMRRFFNPKRYRIVLFDQRGSGKSRPSASLV
                     DNTTWHLVSDIEALREHLKIDRWQVFGGSWGSTLALAYAQKHPERCTEIVLRGIFLLR
                     SFELDWFYQNDLGAAAMFPDFWERYLKPLSPEERKDCMRSYYKRLTSENRETLLEAAR
                     AWSVWESALAYMKVNESYIRQAEDPKFAAAFARIECHYFVNGGFLERPDQLIEDVPKI
                     RHIPAVIVQGRFDVVCPARSAWDLHKAWPEADLRIVPDAGHSAFETSISRELVLATDR
                     FSRA"
     gene            complement(1807..2163)
                     /locus_tag="VEW08_15370"
     CDS             complement(1807..2163)
                     /locus_tag="VEW08_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HYJ42172.1"
                     /translation="MATIFEKIIAGEIPATIVHRDDRVTAFRDISPQAPVHLLIVPNK
                     PIPTVNDVSEDDERTLGHMFVVARDLARAEGIADDGFRLIINCNKHGGQVVYHLHMHL
                     VGGAMLGPMLPGGKHG"
     gene            2320..3336
                     /gene="holA"
                     /locus_tag="VEW08_15375"
     CDS             2320..3336
                     /gene="holA"
                     /locus_tag="VEW08_15375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HYJ42173.1"
                     /translation="MKLSLETLRAHLAGEPARAYLVSGDEALLVGEAADAIRARVKRA
                     GFDEREVHFIERVADWDAVRASANNLSLFGSRKLIEIRLPSGKPGVGGANAIVDLLAN
                     VSPDNVYLMLTGKLEREQNNSAWVKAFESAGAWLAAWPVELARLPQWLRARAATLELE
                     IEDEAVRFIVDRTEGNLLAAQQELEKLKLLASGKRVDLAAVQASIGDSARFDVFQLGE
                     AALGADVPRALRILAGLRSEGVEPTLALWSIAREIRNVWGTTQQGPARGWQKPSAALE
                     NARRRAAKLPYARLAARTSRADRMIKGQHSGDAWDEMALLIVEFAGRRSLPLVGPHTG
                     ARAA"
     gene            3333..3986
                     /gene="nadD"
                     /locus_tag="VEW08_15380"
     CDS             3333..3986
                     /gene="nadD"
                     /locus_tag="VEW08_15380"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HYJ42174.1"
                     /translation="MNTPPGPIGLFGGTFDPIHYGHLRTAFELWQSLKLTEVRFLPTG
                     NPPHRERTLADAQLRVAMVRAAVADQAAFVVDDREVRRAGASYTVDTLTELRNEFPQR
                     SLCLLLGMDAFLGLPNWHRWRELFDLAHVVVAHRPGWKAPTQGPLGELMVDRGTGAVT
                     DLHKASAGRIYVHAVTQLEISSTELRQIIDAGRDPRFLVPDPVREIIRESGCYARPK"
     gene            4000..4512
                     /gene="rsfS"
                     /locus_tag="VEW08_15385"
     CDS             4000..4512
                     /gene="rsfS"
                     /locus_tag="VEW08_15385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HYJ42175.1"
                     /translation="MATKTASRRAAALPKNSIPQTLLRIVNSALDDMKAVNVRVMDVR
                     HLTDITDIVVIASGNSDRHVKSIADRVLQFAKQNDFRPIGVEGAREGEWVLVDLNDVV
                     VHVMLPRVREFYGLEKLWEDVEPPAADHSMEKIVEKVIASKSTPPKRAAPKRAAAPRR
                     KTPARARAAR"
     gene            4522..4962
                     /gene="rlmH"
                     /locus_tag="VEW08_15390"
     CDS             4522..4962
                     /gene="rlmH"
                     /locus_tag="VEW08_15390"
                     /inference="COORDINATES: protein motif:HMM:NF000986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HYJ42176.1"
                     /translation="MRVRIVAVGTRMPGWVTQAYEDYTRRLRSHLRIDLEELQVGKTR
                     ADEEKRLLERAGDDFIVALDEHGKSLTTLELAKWLGQRQQDGRNLAFVIGGPDGLGPE
                     ILKKASLRWSLSALTFPHAMVRVILAEQLYRAHSVLQNHPYHRE"
     gene            4970..5596
                     /locus_tag="VEW08_15395"
     CDS             4970..5596
                     /locus_tag="VEW08_15395"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HYJ42177.1"
                     /translation="MAKQEQEQQPPVLRLASASPRRRQLLDLIGVPHVVLAADIDETP
                     REAEPADAYVVRLAQEKAAAVWSIARDLPVLAADTTVVVDGEILGKPESEAHAHEMLG
                     KLSGREHVVHTGVALISKGIPQVVLSSTRVTFGALSREQIHAYWASGEPQGKAGAYAI
                     QGLGAAFVAGIAGSYTGVMGLPLFETAQLLRAVGIPAWNGALKVEGAE"
     gene            5593..7062
                     /gene="rng"
                     /locus_tag="VEW08_15400"
     CDS             5593..7062
                     /gene="rng"
                     /locus_tag="VEW08_15400"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HYJ42178.1"
                     /translation="MTTEILINVAPRENRAALVENGVLTELFIERQSRRGIVSNLYKG
                     RVQRVLPGMQAAFIDVGLERTAFLHAADIAAPSPENTLIGTPSGEDIRSLVKEGDDIL
                     VQVVKDPMGTKGARLTTFVALPSRLMVYMPRGEGIGVSARIDDEAERARLKSLVTEAV
                     PQQTTGGYIVRTAAQGASMEQIRDDIQYLGRLWQHVKQKGMLAQSGELVYEDLPLPTR
                     ILRDELARGVDRVLVDNATSHVGMLEFAASFMPGSTTSIELYTGQRPIFDLHGVEEEI
                     GKALERSVNLKSGGHLVIDQTESMTTIDVNTGGFVGHRNLEDTIFRTNLEAAVAIARQ
                     LRLRNLGGIIIIDFIDMADVAHRTQVMEALQRALAGDHAQTHIASVSSLGLVEMTRKR
                     TRDSLEHLLCAACPSCEGRGFVRTPETVCHEIFREIVRQSRQFQSRELLILAHQNVID
                     RLLDDESTVLAELEHQVGRPIRLQVETLYGFDQYDVVLA"
     gene            7135..10455
                     /locus_tag="VEW08_15405"
     CDS             7135..10455
                     /locus_tag="VEW08_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42179.1"
                     /translation="MLVTFCLLLLGTLIVVFTRVVAARVPAQRANLEKLITDRTGLAV
                     RFDNVHFAWTLDGMSAVFERVELTDPKQGRVRVVAPALSVEFDTWDYLRHHQFSLGHV
                     KLASPDIEITGDALDAPAVSAGAPRANTPEPTRADEAALVRRFAAWVELMPNGRVEVE
                     GARVHLKRRGDRAARTTFTLSQAVLIRGPSTFNAFGTMLLSQDVGQSLFLSAKLDGLS
                     PGSRVSGDLRLIARRVFLDKISVIAAKGRGTLDARLHLQDGLVQSASWQASARELQFP
                     DDSRTRFDHLTVSGKLERDADDFLLQFADLQLTRGARLERAPNLSARVSVEPGTTRIA
                     RTTLSAERLPFMAAELMSRLLASQFEGRTIAFPGGWTPTSGELRSVHFDSRLKGGGWS
                     LDARLTGADLERTIDHARIGHVAAHLQLSAGGLALTFDPANTMNLRVPGAQEPRALNL
                     GGRLVALNSADTTVRFEDFSMRSDAGTVAAQGEWNGELNDAAARARSLVLTVSNLDRA
                     LLLDGWAMLAADAAIPALLADIEQGVILDGKLTLLPSRNADGNREVNWQRSSGKLTLA
                     GIASSGDDLPSLSAGRGTLEFSRGHTKLLLDGGEFDRLAVTSARLDWPRAGAPRLHAA
                     LQGELTSPVLSRALEAHGLERLTGTVAVEVDARGEKEIRQPDLWRVSARLTDASMPLG
                     GELPPVEKLSGTVRYAGGQLRGLALGGSWLGGPIEIESRRAAPGNTSFAISGVADAGP
                     LLRLLGKAEAANRISGEISWTGTALLVEDDKHGDAWKLSLASTLAGVESRLPAPFDKP
                     VARTLPVSAQLRVDANGIREFEIGAGRDMTMRGAVENGITTAHISVQGVTGEFRRAGN
                     SSDPHLQIERLDMRRSPTVLAAAAAMLPANGELSLGLDDLRYADRSLGAVQATLTRRE
                     DGVGFSLESLQTAQHQLAAQGQCISGETRCRIEFTADTDHLAALLRGVELPAEWPIET
                     LHAAGELTWPVDGQGELTRDLAGQFELETQGRDSSHQLVANATLAEGQITLANVQGTG
                     PDADQVFRGNGRVALLAREYDLTVDYERVSMAASAVPTQARARVARAWNALRGSVARR
                     GWTEVPEARRVQWHGTWD"
BASE COUNT         1899 a         3445 c         3527 g         1873 t
ORIGIN      
        1 ctggcagcgc tctagcggtt tttcgtgggc ccgcgatcca gtcccgccga cgttctgcgc
       61 gtgggcaagg aggcgcacgc atccgtggtc gggctcacgc acaggtttgg cgtagagctg
      121 cgtcagcttg ccgccggcga aatactgccc ggctcattct ggggcgagag tgaagctggc
      181 ctgcgcgcca atgtccttta cgtccgcgct gacacccctc tgcattcgtt gttgcacgag
      241 ctgagccact acgtgtgcat gtcgccggag cgccgcgcca gcctcgatcg cgacgccggc
      301 ggtgatgacg aggaggaatg cgcggtgtgt tacctgcaga tcctgctcgc tgatgagctc
      361 cccatgctcg ggcgcgatcg aatgctcgag gacatggatg cctggggcta tacgttccgg
      421 ctgggttcgt cgcgtgcgtg gttcgagcag gatgcggcgg atgcctgcac ctggttacgc
      481 ggccacggca tcgtcgatga cggcaatcgt ccgacgtttc ggctcaaagg cgctgcattg
      541 agtcggggtg ggtgaaggcg gcgtgccatt caaaaagatt aggagtcgtt tcggaagttc
      601 accttccgcc tccaccaccc gactatcgac cgacggatcc tgcgttgagc agacgcagtt
      661 cccgctagca gctgtaacgg ccgaaaggtg ttggacttta gcgcctgcgc aaaagatttc
      721 ccgcgaaccg gcggctcggt tcgcgtgttt gacagaaccc tcgcgaaaac agcctaggcg
      781 cgggaaaagc ggtcggtggc aagcaccagt tcgcgggaga tgctggtctc gaatgccgag
      841 tggccggcat cgggcacgat gcgcaggtcc gcctcgggcc aggccttgtg cagatcccac
      901 gcgctgcgcg ccgggcacac cacgtcgaag cgcccttgca cgatgaccgc gggaatgtgg
      961 cggatcttcg gcacgtcttc gatcaactgg tcagggcgct cgaggaagcc gccattcacg
     1021 aagtaatggc actcgatgcg cgcgaacgcg gcggcaaact tgggatcttc ggcctgcctg
     1081 atgtaactct cgttgacctt catgtacgcc agcgcgcttt cccagacgga ccaggcgcgc
     1141 gcggcttcga gcagcgtctc gcggttctcg ctggtcagac gcttgtagta cgaccgcatg
     1201 cagtccttgc gctcttcggg cgataacggc ttcagatatc gctcccagaa atccgggaac
     1261 atcgccgccg ccccgaggtc gttctgatag aaccaatcga gctcgaaact ccgcagcagg
     1321 aagatgccgc gcaacacgat ctcagtgcag cgttccggat gcttctgcgc gtaagcgagc
     1381 gccagcgtgg atccccacga cccgccaaac acctgccagc ggtcaatctt cagatgctcg
     1441 cgcaaagctt cgatgtccga gacgagatgc caggtggtgt tgtcgacgag actcgctgag
     1501 ggtctgctct tgccgctgcc gcgctggtcg aacagcacga tgcggtaacg cttcgggttg
     1561 aaaaacctcc gcatcttcgg gtccgtgccg ccacccgggc cgccatgcag gaacaccacg
     1621 ggctttccat tggggttgcc gctttcttcg tagtagagct cgtgtacgtc cgagacgcgc
     1681 aggaatcccg tgttgtaggc cttgatggcc gggtagaaat cccccgcctt gcgtgcgggc
     1741 gccgcggctt tgggcgcgcg cttcttcgat ttcgctgcct tcgatttcac aggggacttc
     1801 ttcttgctag ccatgttttc ctccggggag catcgggccg agcattgcgc cgccgaccag
     1861 gtgcatgtgc aggtggtaca ccacctgccc gccgtgcttg ttgcagttga tgatgagtct
     1921 gaaaccgtcg tccgcaatgc cttcggcgcg cgccaggtcg cgggcgacca cgaacatgtg
     1981 gccgagcgtg cgctcgtcgt cctcgctcac gtcgttgacc gtgggaatgg gtttgttagg
     2041 cacgatgagc aagtgcaccg gcgcctgcgg gctgatgtcg cggaaggcgg tgacgcggtc
     2101 gtcccggtgg acgatggtgg cgggaatctc tcccgcgatg atcttctcga agatggtggc
     2161 catgtggggg cagagaatac gcggcggccc cgagactcgt cgactggcgc gccgcatcgt
     2221 gcccggaccg gaaattgcca atcagtggcc tattcgggtg gcttccgggg cggcaagtcg
     2281 cccataatcc gcaccgaacg tgcagaaatc cccccgccat tgaagctctc gctcgaaact
     2341 ctccgtgcgc acctcgcagg tgagcccgcg cgagcctatc tcgtgtccgg cgacgaggcg
     2401 ctgctcgtgg gcgaggccgc cgatgccatc cgcgcacgcg tcaagcgcgc gggattcgat
     2461 gagcgcgaag tccacttcat cgagcgcgtg gccgactggg atgcggtacg cgccagcgct
     2521 aacaatctat cgcttttcgg gtcgcgcaag ctgatcgaga tccgccttcc ttccggaaag
     2581 cccggtgtgg gtggcgccaa tgccatcgtc gacctgctgg ccaatgtcag tcccgacaac
     2641 gtgtacctga tgctcaccgg caagctcgag cgcgagcaga ataattccgc ctgggtgaag
     2701 gcattcgaga gcgccggtgc ctggctcgcg gcgtggccgg tggagctggc ccgtctgccg
     2761 caatggctgc gcgcgcgcgc cgcgacgctc gagctcgaaa tcgaagacga ggcggtgcgg
     2821 ttcatagtcg atcgcaccga gggcaacttg ctcgccgcgc agcaggaact cgaaaagctc
     2881 aaactgctgg caagcggcaa acgtgtcgat ctcgccgcgg tgcaggcgtc gattggcgac
     2941 agcgcgcgct tcgacgtatt ccagctcggc gaggcggcgc tcggcgccga cgtgccgcgc
     3001 gcattgcgca tccttgccgg tttgcgcagc gagggcgtgg agcccacgct cgcgctctgg
     3061 tcgatcgcgc gcgagattcg caatgtctgg ggcaccacgc agcagggccc ggcgcgtggc
     3121 tggcagaaac cgtcggccgc gctcgagaat gccaggcgcc gcgcggccaa acttccctat
     3181 gcgcggctcg cggcgcgaac ctcgcgcgcc gaccgcatga tcaagggcca gcacagcggc
     3241 gatgcctggg atgaaatggc gctgctgata gtcgaattcg ccggacgccg ttccctgccg
     3301 ctcgttgggc cacacaccgg agcgcgtgcc gcttgaacac acccccgggt cccatcggtc
     3361 tgttcggcgg tactttcgat ccgatccatt acgggcatct gcgcacggcc ttcgagctct
     3421 ggcagtcgct gaaactcacc gaggtccgct tcctgcctac cggcaatccg ccgcatcgcg
     3481 aacgcacgct cgccgacgcg cagctgcgcg tggccatggt gcgcgcggcg gttgcggatc
     3541 aagccgcctt cgtcgtcgac gatcgcgaag tgcgtcgcgc aggcgcctcg tacacggtgg
     3601 atacgttgac ggagctccgc aatgaattcc cccagcgctc gctgtgtctg ctgctcggca
     3661 tggacgcttt tctgggcctg ccgaactggc atcgctggcg cgagctgttc gacctggcgc
     3721 atgtcgtggt tgcgcaccgt cccggatgga aggcgcccac ccagggaccg ctcggtgagc
     3781 tgatggtgga tcggggtacg ggcgcggtga ccgacctgca caaggcgtca gccggccgaa
     3841 tctacgtaca tgccgttacc cagctcgaaa tttcttcgac agaattgcgc cagatcatcg
     3901 atgcgggccg tgacccgcgg ttcctggtac cggaccccgt gcgcgagatc attcgcgagt
     3961 ccgggtgtta tgctcgcccc aagtaaccca cggagagaaa tggccaccaa aactgcttcc
     4021 cgccgcgccg cggcgctgcc caagaattca atcccgcaga ccctgttgcg catcgtcaat
     4081 agtgcgctcg atgacatgaa agccgtcaac gtcagagtca tggacgtccg gcacctcacc
     4141 gacatcaccg acatcgtcgt catcgcctcc ggcaattccg accgtcacgt gaaatccatc
     4201 gccgatcgtg tgctgcagtt tgccaagcag aacgacttcc gtcccatcgg cgtggaaggc
     4261 gcgcgcgaag gcgagtgggt gctggtcgat ctcaacgacg tcgtggtgca cgtgatgttg
     4321 ccgcgcgtcc gcgagttcta cggactcgag aagctctggg aagacgtcga gccgcctgcg
     4381 gccgatcatt cgatggaaaa gatcgtcgaa aaagtcatag cgtccaaatc gactccgccg
     4441 aaacgcgccg cgccgaagcg cgccgctgcg ccacgccgca agacgcccgc gcgagctcgc
     4501 gccgctcgtt agccttgccg catgcgcgtt cgcatcgttg cggttggaac gcgcatgccc
     4561 ggatgggtca cgcaggcata cgaggactac acgcgccgcc tgcgttccca cctgcgcatc
     4621 gatctcgaag aacttcaggt cggcaagacc agggccgacg aagagaagcg cctgctggag
     4681 cgcgccggtg acgacttcat cgtggcgctc gacgagcatg gcaaatccct aaccaccctc
     4741 gagctcgcga aatggctggg ccagcgccag caggatggcc gcaacctcgc attcgtgatc
     4801 ggcggtcccg acggtctggg gccggaaatc ctgaaaaagg ccagcctgcg atggtcactg
     4861 tcggcactga ccttcccgca cgccatggtt cgcgtgatat tggcggaaca gctgtatcgc
     4921 gcccattcgg tgctgcagaa tcacccgtat catcgcgagt gaagggtcga tggccaaaca
     4981 agaacaagaa caacaaccac cggtgttgag gctggcctcg gcctccccgc gacgccggca
     5041 gttgttagac ctgattggcg tgccgcacgt ggtgctggcc gccgatatcg acgaaacgcc
     5101 gcgcgaggcc gagcccgcgg atgcctatgt cgtgcgcctc gcgcaggaaa aggccgctgc
     5161 ggtctggtcc atcgcgcgcg atctcccggt gctcgccgcg gacaccacgg tggtggtcga
     5221 cggcgaaatt ctcggcaagc ccgagtccga ggcgcatgcg catgaaatgc tcggcaaact
     5281 ctccggcagg gagcatgtcg tgcacacggg cgtcgcgctg atcagcaaag gcatcccgca
     5341 ggtggtgctc agctccaccc gggttacatt cggcgcgttg tcgcgggagc agatccacgc
     5401 ctactgggcc agcggagaac cgcagggaaa agccggggcg tacgcgatcc aggggctcgg
     5461 cgcggcattt gtcgccggca ttgccggcag ctacaccggc gtcatgggtc tgccactatt
     5521 cgagaccgcg cagttattgc gggcagtcgg aatcccggcg tggaacgggg cgctgaaggt
     5581 cgagggggcg gagtgacgac ggaaattctc atcaatgtgg cgccacgcga aaaccgcgcg
     5641 gcgctcgtgg aaaacggcgt cctgacggag ctgttcatcg agcggcagag ccggcgcggc
     5701 atcgtcagca atctgtacaa gggccgtgtg cagcgcgtgc tgcccggcat gcaggccgcg
     5761 ttcatcgacg tcgggctcga gcgcaccgcc ttcctgcacg cggcggacat cgcggcaccc
     5821 agcccggaaa acacgttgat cggcacaccg agcggcgagg acatccgctc gctcgtcaag
     5881 gaaggcgatg acatcctggt gcaggtggtc aaggacccga tgggcaccaa gggtgcgcgc
     5941 ctaaccactt tcgtcgcgtt gccgtcgcgc ctcatggtct acatgccgcg cggcgaaggc
     6001 atcggcgtct ccgcgcgcat cgacgacgag gccgaacgcg cacgcctgaa gtcgctggtg
     6061 accgaagccg tgccacagca gaccactggc ggatacatcg tgcgtaccgc ggcgcagggt
     6121 gcgtcgatgg aacagattcg tgacgacatc cagtatctcg ggcgcctgtg gcagcacgtg
     6181 aaacagaaag ggatgctcgc gcagtccggc gaactggtgt acgaagacct gccgcttccc
     6241 acgcgcatcc tgcgcgacga actggcgcgt ggtgtcgacc gcgtgttggt cgacaacgcc
     6301 acttcgcatg tcggcatgct cgagttcgcc gccagcttca tgcccggcag caccacttcc
     6361 atcgagctgt acaccggcca gcggccgatt ttcgacctgc acggggtcga agaggaaatc
     6421 ggcaaggcgc tcgagcgcag cgtcaatctc aaatccgggg ggcacctggt catcgaccag
     6481 accgagtcga tgaccacgat cgacgtcaac actggcggct tcgtcggcca ccgcaatctc
     6541 gaagacacga tttttcgcac gaacctcgaa gcggccgtgg ccattgcgcg gcagctcagg
     6601 ctgcgcaatc tcgggggcat catcatcatc gatttcatcg acatggcgga cgtggcccac
     6661 cgcacccagg tcatggaagc actgcagcgc gcgctggcgg gtgaccatgc gcagacccac
     6721 attgccagcg tctcctcgct cggcctggtg gaaatgacgc gcaagagaac ccgcgacagc
     6781 ctggaacatt tgctttgtgc ggcgtgtccc agttgtgaag gccgtggctt cgtgcggacg
     6841 cccgaaacgg tctgccacga gatattccgt gaaattgtgc gccagagcag gcagttccag
     6901 agccgcgaac tgctaatttt ggcccaccag aatgtgatcg accgcctgct ggatgacgaa
     6961 tccacagtgc tcgcggagct ggagcaccag gtggggcggc cgataaggtt acaagtcgaa
     7021 acgctctacg gcttcgatca atacgatgtg gtgctggctt gaagaggtga gatgaccaac
     7081 gcaacccttg aacaaagggg ggacgaaagc gactcgcact gggcgagacg cacggtgctg
     7141 gtcacgtttt gcttattgct gctcggcacc ctgatcgtgg tgttcacgcg cgtcgtcgcg
     7201 gcgcgtgttc cggcgcagcg cgccaatctc gagaaactca tcaccgatcg caccggcctt
     7261 gccgtgcgtt tcgacaacgt gcatttcgcg tggaccctcg acggcatgag cgccgtgttc
     7321 gagcgcgtcg agctcaccga tccgaagcag ggacgcgtgc gggtcgtcgc gccggcgctg
     7381 agcgtcgagt tcgacacgtg ggattacctg cgccatcacc agttttcgct ggggcacgtg
     7441 aagcttgcct cgccagacat cgaaatcact ggcgatgccc tcgacgcgcc cgcggtcagc
     7501 gcaggcgcgc cgcgcgcgaa tactcctgag ccgactcggg ccgacgaagc cgcgttggtg
     7561 cgccgcttcg cggcgtgggt cgagctcatg ccgaacggcc gagtggaagt ggaaggggcg
     7621 cgggtgcacc tcaagcgacg cggcgatcgc gccgcgcgca ccaccttcac gttgagccag
     7681 gcagtcctga tccgtggccc ttccacgttc aacgcattcg gcaccatgct gctctcacag
     7741 gatgtgggcc agtccctgtt cctgtccgcg aagctggatg gactcagccc aggttcacgc
     7801 gtctcgggag acctgcgcct gatcgcgcgt cgcgtgttcc tcgacaagat ttccgtcatc
     7861 gccgcaaaag gccgcggcac gctcgatgcc aggctccacc tgcaggatgg cctcgtacag
     7921 tcggcgagct ggcaggcaag cgcgcgcgag ctccagttcc ctgacgattc ccgcacgcgg
     7981 ttcgatcacc tgacggtgag tggcaagctc gaaagggacg cggacgattt cctgctgcag
     8041 ttcgcggacc tgcagctcac gcgcggcgcg cgtctggagc gtgcgccgaa cctgtcggca
     8101 cgggtcagcg tcgaacctgg tacgacgcga atcgcgcgca cgacgctcag cgccgagcga
     8161 ttgccgttca tggctgcgga actcatgagc cggctgctcg catcgcagtt cgaaggtcgg
     8221 accatcgcat ttccgggcgg ctggacgccg accagcggcg agcttcgcag cgttcacttc
     8281 gattcgcgcc tgaaaggtgg cggctggagc ctcgacgcaa ggctcactgg agcggacctc
     8341 gagcgcacca tcgatcacgc ccgcataggc cacgttgccg cgcacctgca gctcagtgcc
     8401 ggcgggttgg cgctgacctt cgatccggcg aacaccatga acctgcgcgt tccgggtgcc
     8461 caggagccgc gagcactgaa tctcggtggg cgcctggtgg cgctgaattc ggcagacacc
     8521 accgttcggt tcgaagactt ctcgatgcgc agcgacgccg gcactgtcgc ggcgcaaggc
     8581 gaatggaatg gcgaactcaa tgacgccgcg gcccgggcca ggtcgctcgt gctcacggtt
     8641 tccaatctcg accgtgcact gttgctcgat ggctgggcga tgctcgccgc agatgcggca
     8701 attcccgcgc tgctggccga catcgaacag ggcgtgatac tcgatggcaa gctgaccttg
     8761 ctgccgtcac gcaatgccga tggcaaccgc gaggtcaact ggcagcgctc tagcggcaag
     8821 ctgacgttgg ccggaatcgc cagttcaggc gacgacctgc cgagcctgtc ggcggggcga
     8881 ggtaccctcg aattctcgcg tggccatacg aagctgctgc tcgacggcgg cgaattcgat
     8941 cgactcgccg tgacgtccgc acgtctcgac tggccgcgcg ccggcgcacc gcggctacac
     9001 gccgcactgc agggcgagct tacctcgccg gtgctgagcc gcgctctcga ggcgcatggg
     9061 ctcgagcgcc tcaccggtac tgttgcggtg gaggtcgatg cgcgcggtga aaaagagatt
     9121 cgccagccgg atctgtggcg cgtcagcgcg cggctcaccg acgcctcgat gcctctcggt
     9181 ggtgaattgc ccccggtcga aaagctctcg ggcaccgtgc gatatgccgg cggacagttg
     9241 cgcgggctcg cgctcggggg aagctggctc ggtgggccca tcgaaatcga atcgcgccgc
     9301 gctgcgcccg gcaatacgag cttcgccatc agtggtgtcg cggacgcggg tccgctgctg
     9361 cgcttgttag gcaaggcgga agccgccaac cgcatcagcg gcgagatctc ctggacgggc
     9421 acggcgctgc tcgtcgaaga cgacaaacac ggcgatgcgt ggaagttgtc actcgccagc
     9481 acactcgccg gtgtcgaaag ccggctcccc gcgcctttcg acaaaccggt tgcacgaacg
     9541 cttcccgtga gcgcgcaatt gcgcgtcgac gccaatggca ttcgtgagtt cgagatcggc
     9601 gcgggccggg acatgacgat gcgtggcgcc gtcgagaacg gaatcactac cgcgcacatc
     9661 agcgtgcagg gcgtgacggg tgaatttcgc cgcgccggaa attcgtccga tccgcatttg
     9721 caaatcgaac gactcgacat gaggcgctct cccacggtgc tcgccgcggc ggcggcgatg
     9781 ttgcccgcga acggtgaact ctccctgggg ctggacgatt tgcgttatgc ggatcgcagt
     9841 ctgggtgcag tgcaggcaac gctgacgcga cgcgaagacg gcgtagggtt ctccctggaa
     9901 tccctgcaaa cggcgcagca ccagctggcc gcgcaaggtc agtgcatcag cggcgaaacg
     9961 cgttgccgca tcgagttcac cgccgacacc gatcacctgg cggcgcttct gcgcggagtc
    10021 gagttgcccg cggagtggcc catcgagacg ctgcatgcgg ccggtgagct gacctggcca
    10081 gtggacggcc agggcgaatt gacgcgtgac cttgccggac agttcgaact cgagacgcag
    10141 gggcgcgaca gcagtcatca gctcgtcgcc aatgccacgc tcgccgaagg ccagatcacg
    10201 ctcgcaaacg tgcagggaac gggtcccgat gccgaccagg tgtttcgtgg caacggccgt
    10261 gtggcgctgc tggcgcgtga atacgacctg accgtggact acgagcgagt ctccatggcc
    10321 gccagcgccg ttcccacgca ggctcgtgcg cgtgtggcac gtgcctggaa cgccctgcgt
    10381 ggttcagtgg cccgccgcgg gtggaccgaa gtgcccgaag cacgtcgcgt gcagtggcac
    10441 ggcacctggg actaatgggg ggcattcctc gtttcctagc aaacggggac ggggctcgta
    10501 ggtggagggt cgagcgacgg gcggccctgc aagcgatgag actcgctatt cgccgggccc
    10561 ttcgttcagg gtggcatcgc cagttcgcgg gccgccgaca cacgcgaaga caccttatgg
    10621 gtggttggat ctcaggatgg cgaagagtcc cgaagacgca gggatcggct tcgtgcctaa
    10681 cgaggccagg tatcccggcc cactcgctca tcgcatgcag ggccacccgt cgctcgatcg
    10741 tgca
//
LOCUS       DASQJS010000393        12630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1198731, whole genome shotgun sequence.
ACCESSION   DASQJS010000393 DASQJS010000000
VERSION     DASQJS010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12630
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198731"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VEW08_15410"
     CDS             <1..886
                     /locus_tag="VEW08_15410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYJ42180.1"
                     /translation="IMNSQGFSLGMLYSFLAFRAMFTERVLMLIARASQFGMLSLYAE
                     RLSDFVLEKPEVAPGSEVDAELAGNIVLSEISFRYGATDPMVLDNVNLEIHAGEFVAI
                     TGVTGGGKSTLFKLLLGLQQPTEGTIHLNGERATPALWQAWRAHIGVVRQDDQLFSGS
                     LAENISFFDPDIDMVRVREAAAAASVSQDIERMPMKYLTLVGDMGSSLSGGQKQRILL
                     ARALYRKPKMLLLDEGTANLDPATEELIADMIAGMPITRIVIAHRPALIDRASRVLKV
                     DRGRIETVRAHVRLAQRA"
     gene            complement(892..1797)
                     /locus_tag="VEW08_15415"
     CDS             complement(892..1797)
                     /locus_tag="VEW08_15415"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HYJ42181.1"
                     /translation="MKGIRRVAPLRVVQGRIESEPGVVAQLRGMSLFWSQWMPQFYNA
                     AAVRWLRDDWRVDVVRAAMGVHRGGYLEHPRREAAKVRAVIEAAIDCGIYVIVDWHAH
                     LQAREGACRFFARIAREYARYPNVIYETWNEPDSRYDWARTIKPYHERVISSIREHDE
                     HNLVVAGTPHWSHRVDIAAADPLALPNVAYTLHFYAGSNAQSLRTLAAAALRGGAALM
                     VTEWGTCRADATGPVHEEECARWFDFLEHEKLGYVNWSVADKAETAAALLPGAQAVGE
                     WSETELSASGRFVRDQLRRMSARSR"
     gene            1863..3329
                     /locus_tag="VEW08_15420"
     CDS             1863..3329
                     /locus_tag="VEW08_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42182.1"
                     /translation="MSPRYSAALDPSVLGEHIAVPFEVRRLPHARLLWLNERWLIEAG
                     FAHDTQARAEFAARMLRHFAVTSRVSDLPGTSACQHTLYADRYGGSGGALHGGSGRCG
                     SKDGFIAKGIGKTPLVDEGVDRYHKNGRMSVAEATRETVNAEIANRELPWGAVPTVAI
                     IDAGFDFPYGESAEPRRAAIVVRPQFIRPAHFERSIFFGTGGHRDSAQFLDALRVRDA
                     TRAVVAAPSTYPQLLEMFRRFAQQIGVARARRLWQGRFLSSNVSIDGALVDFGSFRAV
                     PNWRRTIGVAGECFGAEIEQLRQTFLSVAGFFGKYAPQALTGLDPRPYLRELVALERA
                     SFLDACLSGFGAQKMSDAAPARELVACIEAYYRCQQAARTGVDVAHDCRWIHDAFTGR
                     TAVRPGAGHEQAAEAQLLAAIRTAEASRELGDVSLRRAAEFFRPRALLTYDASNRRAR
                     RIERLAGRSDAPRLVQRYIAREIAMSLPPVSKQLLAVG"
     gene            3336..3797
                     /locus_tag="VEW08_15425"
     CDS             3336..3797
                     /locus_tag="VEW08_15425"
                     /inference="COORDINATES: protein motif:HMM:NF014813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HYJ42183.1"
                     /translation="MDISKNFDYPENDFERQLSANFGHAIRVLASCDRRTEALQPLGI
                     HLWPAVRLNQIGFLYNSKGSPVAFATWAFVTDEVAQSMLADPNYNMDISEWNEGDQLW
                     LVDFVAPFGEARNLMRKLCTVYLPGAWRVRGIRRYSKGKQPRVVDFKRKAA"
     gene            3794..5404
                     /locus_tag="VEW08_15430"
     CDS             3794..5404
                     /locus_tag="VEW08_15430"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HYJ42184.1"
                     /translation="MSIVGYKAAGHDGAVCCIENGVLRYSIEGEKDSGARHALIGPEE
                     FKSILDKWQTNPSVVCSDSRSMDTNLLEYHGISLDEILWSRASAAGREFEFASVPHEL
                     AHITCAYALSDLPEGQEFYALVWEGYLGRLYHVDKNFLISKLEVMDYVGVRYSFPYHA
                     TGRDGIYGHSAAGKIMALAGLAKPDDVRQPQLKRITDLLLDSWVTHARHERISLNGDP
                     DLLYRELSYLDGKAVDSPEFVALCKALQDGIFNRFFEVARRNVSRKLPLLIAGGCALN
                     CDWNTQWRNCGLFSTVFVPPVPSDCGIAIGVAAAINYIRTGRMKLRWDVYAGEEFVEE
                     NGHLAEQGFRERELDLQSLCEGMLERDWVVAWVQGRYEMGPRALCHRSLIAAPFRTEI
                     RDRLNAIKKREYFRPVAPVCLEETVGEHFDWRGSSPHMLYFQNVSSPQLRTTTHLDGT
                     ARVQTINELQDRATYSLLQEMRKASGFGVACNTSLNFLGKGFINRSSDLAKYAGEQKL
                     DAVVVNDRMYVNLAADQLPLPRVAQTRF"
     gene            complement(5401..6387)
                     /locus_tag="VEW08_15435"
     CDS             complement(5401..6387)
                     /locus_tag="VEW08_15435"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ42185.1"
                     /translation="MRRRVRQRNSLALLTLLSVLGGCSGGSNDYGAEEKPPLVLGFSQ
                     ITAGANWNTANTESIRKAARDAGVVLRLEDAHRSQETQMAQLRSFVSQRVDMIAFSPV
                     VANGWETVLREIRSAGIPVILMDRTIEISDESLYVSLIGSDFVEEGRRAGRWLLEHTR
                     DVPGEIDIVEVQGTVGSAPANDRKQGFAEIISADRRFHIIRSQSGDFDRARAREVMAD
                     FLQAEGRRIRVLFAHSDTMALGGIEAIEAAGFKPGSDILVISIEGSRKALEAIVAGKL
                     NVTVECSPLLGPQLMAVVRELAAGNPVPRRVITQESVFTRENAALEMPNREY"
     gene            complement(6447..7310)
                     /locus_tag="VEW08_15440"
     CDS             complement(6447..7310)
                     /locus_tag="VEW08_15440"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HYJ42186.1"
                     /translation="MSPEQATRSGGSFRLRVIGAVLFGLALNNAGAEPISFRLIPREL
                     VESRLREYSGTNIERGARLKRMFADAGCGEHLSEQPVKWSKAPNVICVLPGSSDRVII
                     VGAHFDRVPGSAGVADNWSGASLLPSLYASVNIEPRRHTYIFIGFTDEETGLVGSRFY
                     ARKMTPEQVAATDAMINLDTLGLAPTEVWTHRSDIRLTQALHAVAKHLKVPLSGVNFE
                     RVGSTDSESFKARKIPRITIHSLTQKSENDGILHSRKDKLSAMNLDDYYDTYHLLALY
                     LAFLDHFFDVP"
     gene            7484..7633
                     /locus_tag="VEW08_15445"
     CDS             7484..7633
                     /locus_tag="VEW08_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HYJ42187.1"
                     /translation="MNLHITIGPLLSLIAGILIFIVPRLLNYIVAIYLIAMGLIGLFG
                     TGAFR"
     gene            complement(7647..8192)
                     /locus_tag="VEW08_15450"
     CDS             complement(7647..8192)
                     /locus_tag="VEW08_15450"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HYJ42188.1"
                     /translation="MSGVFHLVLDPMRRLRIAAVVIIAFSPGAHAGDAVVGRASPADI
                     SERVVELVNAARSNSRRCGSERYAAAPSLRVSQKLNKAAAGHARDMARRKFFEHRGSD
                     GSQPKDRVLRAGYQPRLTGENIAYGPESAEEVVAGWLASPGHCANIMDSRFHDIGVAL
                     NTGKKRGQIYWVQNFGAPILK"
     gene            8524..9399
                     /locus_tag="VEW08_15455"
     CDS             8524..9399
                     /locus_tag="VEW08_15455"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HYJ42189.1"
                     /translation="MAHTLDVSLGDLRNEATPSELVEFEPATRVLGDLELQAKYLPRR
                     PSLLPRLMTAINNDSNSMRQMAKIIGEDGTLLGGLLKLSNSAFYGVRRHKPIESLEKA
                     VALVGTDGLRSLMATALLHPVMTGTRGPFSSFAEATWEHSQYSAACAELHATHIEGAD
                     AFSARLLVLLRGLASNTVFRIAREHCLAADNQAPAAMAKLLDDWVPPMSLRIAEDWQL
                     PKDLCDVLSAPAAPGLARSLRFGQLAGALLVLVNRGHVRELSARAIVLADDHRRTQVD
                     KLWSRLAIVHLTAAR"
     gene            complement(9490..11238)
                     /locus_tag="VEW08_15460"
     CDS             complement(9490..11238)
                     /locus_tag="VEW08_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42190.1"
                     /translation="MRSDEKLLKRATFPGFPARHRLVLAVCAIAFVACARQDEKPAAS
                     TAPAAAPVPSGPHADFIPTQAQLDNFIAEGADPTVRKISAVDYYLHYRLMQATGIEKE
                     LGGEEKTVAALKALGEEYERRMRSMQIELPKMIKATAFTGEGMSSGFMGMGMGSFLGF
                     IGSGLGTAVAGNLSDAELIELNKKGGIKHGHDSGSAEFTFDKDGSMTQSMEFEVKENG
                     LNGKVKMKTRMSACPDADGKVTVDIDVDSQMSVSGKPGTGGYVHSTMKYERYLDDDAH
                     LIDTGDGGAFNNHIKMGGFENFQSQSFEVTTGRERGGKEIFVEHAESGYSIFRKDEVE
                     KSFKLIQAADFLQTIVAESMLRGLATSTGSPWESGRCIDLKVTSDPGKRKGIRPSTAF
                     DLEAIPRVKSDGSAAGGTVTATLNGGSSLQPASGKVKADAKYGYTGPEKKNETASIDF
                     ESRSKRGVGKASLAFDTKTGRPYRMEGGAGDFHGVGTACDLEQPFTVAGGGNTVSFVP
                     SSRDGGTYTYAGSMAGFGVHGYGTYTVKYADDVAVKIVAQGPGTVETPIGPQTGEGTE
                     IYTLTPLGEGEECKSP"
     gene            complement(11308..12261)
                     /locus_tag="VEW08_15465"
     CDS             complement(11308..12261)
                     /locus_tag="VEW08_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42191.1"
                     /translation="MKFAMALLVICATSAHAEVLVPKGAKGTLTVEYVFTSAGKYTTG
                     KKEAIDTWSVKRNVTMTTQYVADPPQAFGALHQDDGKQKADMATLQAKTTSAHKKLEP
                     MANDVMALAAKCGITMDGPDVSAAEEKAQEACMEKAAAEYGNNMEMTPEIKSAGDDIA
                     AANKLASGTRFQLWRSTSQSGTYSVDETISKQVFEMTCTETKICKRVVTNKGGGAIPG
                     GKSASASMIEVDSTGRDMVLQLPMPAVPLPITRTVTTTMIDDDAKGGPTFASLWPSNS
                     KPVTVAIPGDLKIVSGTQTTTVPGSQSEGGTLTVNWKFTRQ"
BASE COUNT         2187 a         4039 c         3910 g         2494 t
ORIGIN      
        1 catcatgaat tcgcaaggct tctcgctggg catgctgtac tcgttccttg cattccgcgc
       61 catgttcacc gagcgcgtgc tcatgctcat cgcgcgcgca tcgcagttcg gcatgctttc
      121 gctctacgcc gagcgcctca gcgacttcgt gctcgagaag ccggaagtgg cgccgggcag
      181 cgaagtcgat gcggagctcg caggcaacat cgtgctgtcg gagatttcgt tccgttacgg
      241 cgcgaccgac cccatggtgc tcgacaacgt caacctcgag atccacgccg gcgaattcgt
      301 ggcgatcacg ggcgtgaccg gcggaggcaa gagcacgctg ttcaagctgt tgttaggctt
      361 gcagcagccc accgaaggaa caatacatct caacggtgag cgcgcaacgc cggccctgtg
      421 gcaggcgtgg cgcgcccata tcggcgtcgt gcggcaggac gaccagctgt tctccggttc
      481 gctcgccgaa aatatttcgt tcttcgatcc ggacatcgac atggtgcggg tgcgcgaagc
      541 ggccgcggcc gcttcggtga gccaggacat cgagcgtatg ccgatgaaat atctcaccct
      601 ggttggcgac atggggtcga gcctgtcggg cggtcaaaag cagcgcatcc tgttggcccg
      661 cgcgctgtat cgcaagccga agatgttgct gctggatgaa ggcacggcca acctcgatcc
      721 ggccaccgaa gagctgatcg cggacatgat tgccgggatg cccatcacgc gcatcgtgat
      781 tgcgcaccgg ccggcgctca tcgaccgtgc ctcacgggtg ttgaaggtgg accgcggccg
      841 catcgagacg gttcgcgcgc acgtccgact ggcgcagcgc gcgtgaagaa ttcagcgcga
      901 gcgcgcgctc atgcggcgca gctggtcgcg cacgaagcgg ccggatgccg acagctccgt
      961 ttcgctccat tcgcccaccg cctgcgcgcc cggcagcagc gctgccgccg tctcggcctt
     1021 gtcggcgacc gaccaattca catagcccag tttttcgtgc tcgaggaagt cgaaccagcg
     1081 cgcgcattcc tcttcatgga ccgggcccgt ggcgtcggcg cggcaggtgc cccattcggt
     1141 gaccatgagc gcggcaccgc cacgcagggc agccgcggca agcgtgcgca agctctgcgc
     1201 attcgaaccg gcgtagaagt gcagcgtgta ggccacgttc ggcagtgcca gcggatccgc
     1261 ggccgcgata tcgacgcgat gcgaccagtg cggggtgcca gcgaccacga gattgtgctc
     1321 gtcgtgttcg cgaatgctgg agatgacgcg ctcgtggtaa ggcttgatgg tcctggccca
     1381 gtcgtagcgg ctgtccggct cgttccaggt ttcgtagatg acattgggat atcgcgcata
     1441 ttcacgggcg atgcgcgcaa agaagcggca tgcgccctcg cgtgcctgca ggtgcgcgtg
     1501 ccagtcgacg atcacgtaga tgccgcagtc gatggccgcc tcgatgacgg cacgcacctt
     1561 ggccgcttcg cggcgcggat gctcgagata gccgccgcga tgaacgccca tggcggcgcg
     1621 cacgacatcg acgcgccagt cgtcgcgcag ccagcgcacc gccgctgcgt tgtagaactg
     1681 cggcatccac tggctccaga acagcgacat gccgcgcagc tgggccacca cgccaggttc
     1741 gctttcgatg cggccctgta ccacgcgcag gggcgccact cgtcgtatgc ctttcacaag
     1801 gcgggcgatg ctaactaccg tggaggcaat gtgcaatcgt ccgtgcggcg agggtggcga
     1861 acatgagccc gcgctattcg gccgcactgg atccgtccgt gctgggcgag cacatcgccg
     1921 tgccgttcga ggtgcgccgc ctgccgcacg cgcggctgct gtggctcaac gagcgttggt
     1981 tgatcgaggc cggtttcgca catgacacgc aagcgcgcgc tgagttcgcg gctcgcatgc
     2041 tgcgacattt cgcggtgact tctcgggttt ccgacttgcc gggcacgagc gcctgccaac
     2101 acactttgta cgccgaccgt tatggcggct cgggcggcgc gctgcatgga ggcagcggcc
     2161 gctgcggtag taaggacggg ttcatcgcca agggcatagg caagacgccg ctggtcgatg
     2221 aaggcgtcga ccgctatcac aagaacggac gcatgtcggt ggcagaggcc acccgcgaga
     2281 ccgtcaacgc cgagattgcc aaccgtgagt tgccttgggg cgcggtgccg accgtggcca
     2341 tcatcgatgc cggcttcgac ttcccctatg gggagagcgc cgagccacgc cgtgccgcta
     2401 tcgttgttcg gccccagttc atccggcccg cgcatttcga acgcagcatc ttcttcggca
     2461 cgggtggtca tcgcgattcc gcgcagttcc tggacgcgct gcgggtgcgc gatgccacgc
     2521 gcgcggtcgt ggcggcgccc tcgacctatc cccagctact cgaaatgttc aggcgcttcg
     2581 cccagcagat cggcgtggcg cgcgcgcgcc ggctttggca gggtcgcttc ctgtcctcga
     2641 atgtatcgat cgacggtgca ctcgtggact tcggctcgtt tcgtgcggtg ccgaactggc
     2701 gccgcaccat cggtgtcgca ggcgaatgct tcggcgcgga aatagagcag ttgcgccaga
     2761 cgtttctctc cgtggccggc ttcttcggca agtacgcgcc gcaggccttg accggtctcg
     2821 atccgcggcc ctatctacgt gagctcgtgg cgctcgagcg cgcgagtttt ctggacgcct
     2881 gtctttcggg gtttggcgca cagaagatga gcgacgcggc gcccgcgcgc gagctcgtgg
     2941 cctgcatcga ggcctactac cgctgccagc aggcggcacg cacgggcgtg gatgtcgctc
     3001 atgactgccg ctggattcac gacgccttca ccgggcgcac ggcggtacgg ccgggagccg
     3061 gtcacgaaca ggcggccgaa gcccagttgc tcgcggcgat tcgaaccgcg gaagcctcgc
     3121 gggaattggg tgacgtaagc ctgcgcaggg cggccgagtt tttccggccg cgcgcgttac
     3181 tcacgtacga cgcatcgaat cgccgcgctc gacgcatcga gcggctcgcc ggtcggagcg
     3241 acgcgccgcg gctggtgcag cggtacatcg cgcgcgagat cgccatgagt ttgccacccg
     3301 tgagcaagca acttctggcc gtgggatgag cgtcggtgga catcagcaaa aacttcgact
     3361 atcccgagaa cgacttcgaa cggcagctct cggcgaattt cggccatgcc atccgcgtgc
     3421 tggcaagttg tgatcggcgc accgaggcgc tgcaacctct cggcatccac ctgtggccgg
     3481 cagtgcgctt gaatcagatc gggtttctat acaactccaa gggctcgccc gtggcattcg
     3541 ccacctgggc ctttgtcacc gatgaggtgg cgcagtcgat gctcgcggac ccgaactaca
     3601 acatggatat cagcgaatgg aacgagggcg atcagctgtg gctggtcgac ttcgtcgcgc
     3661 ccttcggcga ggcgcgcaat ctcatgcgca agctttgcac cgtttatctg ccgggcgcct
     3721 ggcgcgtgcg cggcattcgc cgctattcga aaggcaagca accgcgggtc gtggacttca
     3781 aacggaaagc ggcatgagca tcgtgggcta caaggccgca ggccacgacg gagcggtgtg
     3841 ctgcatcgag aacggagtgc tgcgctattc gatcgagggc gagaaagatt ccggcgcgcg
     3901 ccacgcgctc atcgggcctg aagaattcaa atccatcctg gacaagtggc agaccaatcc
     3961 ctccgtggtc tgcagcgaca gccgatcgat ggacaccaac ctgctcgaat atcacggcat
     4021 cagcctggac gagatcctct ggagccgcgc ctcggccgcc gggcgcgagt tcgaattcgc
     4081 atcagtgcca cacgagcttg cgcacatcac ctgcgcgtac gcgctcagcg atctgccgga
     4141 aggacaggag ttctatgccc tcgtctggga ggggtacctc ggtcgtcttt atcacgtcga
     4201 caagaacttc ctcatctcca agctcgaagt catggactac gtcggtgtcc gttattcctt
     4261 tccttatcac gcgaccggca gggatggcat ttacggtcat tccgcggccg gcaagatcat
     4321 ggcgctcgcc ggtctcgcga agccggacga cgttcggcag ccgcagctca aacgcatcac
     4381 cgatctgctg ctcgattcgt gggtgaccca tgcccgtcac gaacgaatct ccctcaatgg
     4441 cgatcctgat ctgctctatc gcgagctttc ctatctggac ggcaaggccg tcgacagccc
     4501 cgagttcgtc gcgctctgca aggcgcttca agacggcatc ttcaaccgct tcttcgaggt
     4561 tgcgcggcgc aatgtcagtc gcaagctgcc attgctgatt gcgggcggtt gtgcgttgaa
     4621 ctgcgattgg aatacgcagt ggcgcaactg cggcttgttc agcacggtct tcgtgccgcc
     4681 agtgccgagc gattgcggga tagcgattgg agtggccgcc gcgatcaact acatcaggac
     4741 cgggcgcatg aagctgcgct gggatgtata cgcaggcgaa gaattcgtgg aggagaatgg
     4801 gcacctcgcg gagcaaggct tccgtgagcg ggagctcgac cttcagtcgc tgtgtgaagg
     4861 catgctggag cgcgactggg tcgtcgcctg ggtgcagggc cgctacgaaa tgggcccaag
     4921 ggcgctgtgt catcgctcgc tgatcgccgc accgttccgg accgaaatcc gggaccgcct
     4981 gaacgccatc aagaagcgtg aatatttccg gcccgtcgcg cccgtgtgcc tggaggagac
     5041 ggttggcgag catttcgact ggcgcggatc gagccctcac atgctgtact tccagaacgt
     5101 cagctcgccc cagttgcgta ccacgacgca cctcgatggt acggcgcgcg tccaaaccat
     5161 caacgaattg caggatcgag ctacctactc gctgctccag gagatgagaa aggccagcgg
     5221 gttcggcgtg gcgtgcaaca cctcgctgaa ctttctcggg aaggggttca tcaatcgttc
     5281 gagcgatctt gcgaagtatg ccggcgaaca gaaactcgat gccgtggtcg tcaatgaccg
     5341 catgtacgtg aacctggccg ctgatcaact ccccttgcca cgcgttgcgc agacaaggtt
     5401 ttaatattcg cgattgggca tttccaacgc ggcgttctcg cgcgtgaaca cggactcctg
     5461 cgtaatcaca cgcctcggaa ctgggttgcc ggcggcgagt tccctgacta cggccatgag
     5521 ctgcggtccc agcagtgggc tgcactccac ggtcacgttg agtttgccgg cgacgatggc
     5581 ctcgagcgct ttgcggctgc cttcgatgga aatcaccagg atgtcgcttc cgggtttgaa
     5641 tcccgcggct tcgatggcct cgatgccgcc cagcgccatg gtatcgctgt gcgcgaacag
     5701 aacgcggatg cggcgaccct cagcctgcag gaaatccgcc atgacttcgc gcgcgcgggc
     5761 acggtcgaag tcaccgcttt gcgagcggat gatgtggaag cgccggtcgg ccgagatgat
     5821 ctcggcgaaa ccttgcttgc ggtcgttggc gggcgccgaa cccaccgtac cctggacctc
     5881 gacgatgtcg atctcgcccg gcacgtcgcg agtgtgctcg agcagccagc gaccggctcg
     5941 ccgaccttcc tcgacaaagt ccgagccaat cagcgaaacg tacagcgact cgtcgctgat
     6001 ctcgatggtg cgatccatga ggatcactgg tattcccgcc gaccggatct cgcgcagcac
     6061 ggtttcccat ccgttcgcaa ccacgggcga gaaggcgatc atgtcgacgc gctggctgac
     6121 gaacgaacgc agctgcgcca tctgggtttc ctggctgcga tgcgcatctt ccagtcgcag
     6181 cacgacgccg gcgtcgcgcg ccgccttgcg gatggattct gtgttggcgg tgttccagtt
     6241 agccccggcc gtgatctgac tgaagccgag caccaacggc ggcttttcct ccgcgccgta
     6301 gtcgttcgag ccccccgagc agcccccgag taccgagagc agcgtgagca gcgcgagaga
     6361 atttctctgt cgaacgcggc gtctcatgtg tggaaatata ctcactcgtc tgcaatgggc
     6421 atgtaggaag ccgcgaggcc tttccttcac ggcacgtcga agaagtgatc cagaaacgcc
     6481 agatacaacg ctaacaagtg gtatgtgtcg taatagtcgt ccagattcat ggccgagagt
     6541 ttgtccttcc ggctgtgcag aattccatcg ttctcgctct tctgcgtcag ggaatggatg
     6601 gtgatcctgg gtatcttccg ggccttgaag gattccgagt ccgtcgagcc aactctttca
     6661 aaattgacgc cgctgagcgg taccttcagg tgcttcgcga cggcatggag cgcttgcgtc
     6721 aggcgtatat ccgaacgatg tgtccatacc tcggtgggcg ccaggccgag tgtgtccaga
     6781 ttgatcatcg cgtcggtcgc cgccacttgc tcgggtgtca tcttgcgcgc atagaaacgt
     6841 gagccgacca ggcccgtttc ttcgtcggtg aatccaatga agatgtaggt gtgccggcga
     6901 ggctcgatgt tcaccgacgc atagagtgac ggcagcagcg aagcgccgct ccagttgtcg
     6961 gccactccag cacttccggg cacgcggtca aagtgagccc cgacgatgat gacccgatcg
     7021 gagctccccg ggagaacaca gatgacgttg ggcgccttcg accacttcac gggctgttcc
     7081 gacaggtgct caccgcaacc cgcgtcggca aacatccgtt tcagtctggc tccgcgttcg
     7141 atgttcgtgc cgctgtattc gcgcagtcgg ctttcgacca attcgcgggg aatcaaacgg
     7201 aagctgatgg gttcagcccc ggcattgttc agcgcgagtc cgaacaacac cgcaccgatc
     7261 acccgaagcc tgaatgatcc accgctccgg gtcgcttgtt caggagacat gttcgagtac
     7321 tgtcgctgac gcagcgaatc ttgtgaagag ccgcgttgcc gcgcgcgtag gtattcgctg
     7381 agggaaactg caggcagccg tggcctcagc ctatgtgcca taccgtacag agcggcgcca
     7441 gccgggatgg gactatcgct tcccaacccg cccggaggcg tccatgaatc ttcacatcac
     7501 gataggcccg ctcctttcac tcattgcggg gatcctgatc ttcatagtgc cccggctgct
     7561 caactacatt gtcgcgatct acctgatcgc catgggcctg atcggactct tcggcaccgg
     7621 cgcctttcgc tgagagcggg agcttttcat ttcaggatgg gcgcgccgaa gttctgcacc
     7681 cagtagatct gcccgcgctt tttgccggtg ttgagagcaa cgccgatgtc gtggaacctc
     7741 gaatccatga tgttcgcgca atgacccggg ctggccagcc atccggcaac gacttcctcc
     7801 gcggactcgg gaccgtacgc gatgttctcg cccgtcaggc gcggctggta gccggcgcgg
     7861 agcacgcggt cctttggctg actaccgtca ctgccgcgat gttcgaagaa tttccttcgg
     7921 gccatgtcgc gcgcgtgacc ggcggccgcc ttgttcaact tctgcgaaac cctgagagac
     7981 ggggccgcag cgtaccgctc ggatccgcat ctccggctgt tgctccgggc ggcattgacc
     8041 agttccacca cgcgctcgct gatgtctgca ggggaagctc gtcccaccac tgcatcgcca
     8101 gcgtgggcgc ccgggctgaa cgcgataatg accaccgccg cgatgcgcag ccgccgcatc
     8161 ggatccagta cgagatggaa gacaccagac atgctcccca gtttccgtgt gccgcactga
     8221 acgcaccgtg aatcgacgcc ggcgcagccg tttgcacggc gttggcctgg cgaatttaca
     8281 tagtgggcct tgctgaaaaa gagcttcagt tcggtcgtgc cccgccgata accaacaacc
     8341 atgattgccg ctatccgctc tgggatcggc tctcttgtgg atcaatggtt cggcgggtcg
     8401 cgcatgagga tgggcccgca ggcgatcaat gatcggcagg gaagccgagc acagccttgc
     8461 gccaagggtc ccgtcgacgg tacccaatct acgagtgcgc ctgcggtcgc catgcggcga
     8521 ttcatggctc acacgctgga tgtgagtctt ggcgatctgc gcaacgaagc cactccatcg
     8581 gagctcgtcg agttcgagcc cgccacgcgc gtgctcggtg acctggaact gcaggcgaaa
     8641 tatcttccgc gccggccgtc gctgttgccg cggctcatga ccgccatcaa caacgactcg
     8701 aactcgatgc gccagatggc gaagatcatc ggcgaagacg gcacgctgct gggaggtttg
     8761 ctgaagctgt cgaacagcgc cttttacggg gtacgccggc acaaaccgat cgaaagcctg
     8821 gagaaggcgg ttgcgctcgt gggcacggac ggactgcgct cgctgatggc caccgctctg
     8881 ttgcacccgg tcatgactgg cacgcgagga cctttctctt cgtttgccga agccacctgg
     8941 gagcacagcc agtactccgc cgcatgcgcg gagctgcatg cgactcacat cgagggcgcc
     9001 gatgcgttca gcgcgcggct cctggtgttg ctgcgcggcc ttgcgagcaa taccgtgttc
     9061 aggatcgcga gagaacattg tctggccgca gacaatcaag cgccggcggc catggccaaa
     9121 ctgctcgacg actgggtgcc accgatgtcg ctgcgcatcg ccgaggactg gcagctaccc
     9181 aaggacctgt gcgatgtgct ctcggcgccc gccgcgccgg gcctggcgcg ttcgctgcgc
     9241 ttcggacaac tggctggcgc gctactcgtc ctggtgaatc gcgggcacgt ccgcgagctt
     9301 tcggcgcgcg cgatcgtact cgccgacgat caccgccgca cacaagtgga caagttgtgg
     9361 tcgcgtctcg cgatcgtgca cctcaccgcc gcgaggtgac tgcggtgccg ggccgtgact
     9421 gcggtgccgg gccgggaaac cgggataact attgtttccc gcactaccga agcgactcgt
     9481 ccgcaagtgc tagggcgact tgcattcctc gccttcgcca agcggcgtca gcgtgtagat
     9541 ctccgtgccc tcgccagtct gcgggccgat cggcgtttca accgtgccgg gaccttgcgc
     9601 aacgatcttc accgcgacat cgtcggcgta tttcaccgtg tacgtgccat agccgtgcac
     9661 gccaaacccg gccatggagc ccgcataggt gtacgtgccg ccgtcgcgtg aactcgggac
     9721 gaaactgacc gtgttgccgc cacccgctac ggtgaacggc tgctccagat cgcaggccgt
     9781 gcccacgccg tggaaatctc ccgcgccacc ttccatgcga tacgggcgtc ccgtcttggt
     9841 gtcgaacgca agcgaagcct tgccgacgcc ccgcttgctg cgcgactcga aatcgatgct
     9901 ggcggtttcg ttcttcttct cgggaccggt gtagccgtat ttcgcatccg ctttcacctt
     9961 gccgctggca ggctgaaggc tggagccacc gttcagagtc gcagtcaccg tgccgccggc
    10021 cgccgagccg tcgctcttca cgcgtgggat cgcctcgaga tcaaacgcgg tgctcggtcg
    10081 aattcctttg cgcttgccag gatcgctggt gaccttcagg tcgatgcagc gcccgctttc
    10141 ccacggcgag ccagtcgacg tggccaaccc acgcaacatc gactcggcaa caatcgtctg
    10201 caggaagtcg gcagcctgta tcagcttgaa ggatttctcg acctcgtctt tccggaaaat
    10261 gctgtaaccg ctttcggcat gttccacgaa tatttccttg ccgccacgct cgcggcccgt
    10321 ggtgacttcg aagctctggc tctgaaagtt ctcgaaaccg cccattttga tgtgattgtt
    10381 gaatgctccg ccgtcgcccg tgtcgatcag gtgtgcgtca tcatccagat aacgctcgta
    10441 tttcatcgtc gagtggacat agccgccggt acccggcttg ccgctgacgc tcatctgcga
    10501 gtccacgtcg atatcgaccg tgacttttcc gtccgcatcc gggcaggcgg acattcgcgt
    10561 cttcatcttc accttgccgt tgagaccatt ctccttgacc tcgaactcca tcgactgcgt
    10621 catcgagccg tccttgtcga acgtgaattc agccgaaccg ctgtcgtgcc cgtgcttgat
    10681 cccgcctttc ttgttcagtt cgatcaactc tgcatcggac aggttgccgg cgaccgcggt
    10741 gcccaagcca ctaccgatga atcccagaaa gctgcccatg cccataccca tgaagccgct
    10801 gctcatgccc tctccggtga aggcggtcgc cttgatcatc tttggcaatt cgatctgcat
    10861 cgagcgcatc cggcgctcgt attcttcgcc caacgccttc aaggcggcga cggtcttctc
    10921 ttctccgccg agttccttct cgatacccgt cgcctgcatg agcctgtagt gcaggtaata
    10981 gtccacggcc gaaatcttgc gcaccgtggg atcggcgccc tcggcgatga agttatcgag
    11041 ctgcgcctgc gtcggaatga agtcggcgtg cggaccagag gggacgggcg cggcggccgg
    11101 cgccgtgctg gcggccggct tttcgtcctg ccgcgcgcac gcgacgaatg cgatcgcgca
    11161 gacggctagt accagtcggt gccgggccgg gaaaccggga aaagtcgctc gcttcagcaa
    11221 tttttcgtcg ctacgcacga ggtgcacctt cttgttagga attcgacaat tggctcgact
    11281 tttcccggtt tcccggcccg gcaccgtcta ctgccgcgtg aacttccagt tcaccgtcag
    11341 tgtgccgccc tcggactgac taccggggac ggtcgtggtc tgggtgccgg acacgatctt
    11401 gagatccccc gggatcgcca cggtgaccgg cttgctgttc gacggccaca gcgacgcgaa
    11461 tgtcggaccg cccttggcgt cgtcatcgat catcgtcgtg gtgactgtcc tggtgatcgg
    11521 cagaggtacc gccggcatag gcagttgcag aaccatgtcg cgcccggtgc tgtcgacctc
    11581 gatcatcgag gcgctcgccg acttgccacc cgggatcgcg ccgccaccct tgttggtcac
    11641 cacccgtttg cagatcttgg tctccgtgca ggtcatctcg aacacctgct tggaaatggt
    11701 ctcgtcgacc gaataggtgc cgctctgcga cgtggaccgc cacaactgaa agcgcgtacc
    11761 tgacgccaac ttgttcgctg ccgcgatatc gtcgccggcg gacttgatct ccggcgtcat
    11821 ctccatgttg ttgccgtatt cggcggcggc tttctccatg caggcttcct gcgccttctc
    11881 ttccgcggcg ctcacatccg ggccgtccat cgtgatgccg cacttcgcgg caagcgccat
    11941 cacgtcgttc gccatgggtt ccagcttctt gtgcgcgctc gtggtcttcg cctgcagagt
    12001 cgccatgtcc gccttctgct tgccatcgtc ctggtgcagg gcgccgaagg cctgcggcgg
    12061 gtccgccacg tattgcgtcg tcatggtgac gttgcgtttc acactccagg tgtcgatcgc
    12121 ttccttcttc cccgtggtgt atttgcccgc actggtgaac acgtattcca ccgtgagtgt
    12181 gcccttggcg cccttgggca cgagcacttc ggcgtgcgcg gacgtcgcgc agataactag
    12241 caatgccatc gcgaatttca tgagatctcc ttgcgataag ggaaatgccg aaaccggcga
    12301 ggcgaaagct acgcgaaacc tggcacgacc agcggtctgg tcgtggaatt gattccaaat
    12361 cgcatggcag ccaccctgtt tcactcgcct acacactaga agcgtagaaa gcggcgaaac
    12421 cggctcacga aattagctcg gagcggcaat ttgaggcgct accacggcgc cggcttgtag
    12481 tccttcagaa actgccccca cacatgctcg ccggtgagct ggccggcgaa gatcggatcc
    12541 acgatccgcg ccgcgccgtc gatgatgtcc aggggcggtg aaaatcctgc ttcagccttg
    12601 cgcgccgcgt ggacggcggg gtcctcgtcg
//
LOCUS       DASQJS010000394         4216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4338417, whole genome shotgun sequence.
ACCESSION   DASQJS010000394 DASQJS010000000
VERSION     DASQJS010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4338417"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VEW08_15470"
     CDS             <1..379
                     /locus_tag="VEW08_15470"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HYJ42192.1"
                     /translation="ADLEAAVGVSNEYAPEHLIIQTRDARALLAQVSCAGSVFLGAWS
                     PESMGDYCSGTNHVLPTFGYARSYSGVSLLDYQKRITVQELSADGLRALGPTAITLSG
                     MEGLDAHGNAVKVRLESLAGGAA"
     gene            376..1425
                     /gene="hisC"
                     /locus_tag="VEW08_15475"
     CDS             376..1425
                     /gene="hisC"
                     /locus_tag="VEW08_15475"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HYJ42193.1"
                     /translation="MTDILALARPDIIALKAYSHASWDPAFERLHANELPWRAETDRS
                     IAGLNRYPEPHPHALAQRLADLYGVPDGKLLPGRGSDESIDLLVRGFCRAGVDNVVIC
                     PPTFGMYAVAARIQGAEVREVPLLPERGFALDTAHVLAACDANTKIVFLCSPNNPTGN
                     AMEPAAIEQLLATLANRSLVVVDEAYIEFSGDASLATALTHFPNLVVLRTLSKAFGLA
                     GARVGSLIAAREIVSLLAKVIPPYSIPQLTIEAVLATLEGPQLAIQRERVAQIRAERE
                     RLRAALANCGSVQKIWPSAANFLLVDFADAEVILGAARAAKLLIRDMRNVSPHSLRIS
                     VGSPEQNDRLIRSIP"
     gene            1431..2486
                     /gene="hisB"
                     /locus_tag="VEW08_15480"
     CDS             1431..2486
                     /gene="hisB"
                     /locus_tag="VEW08_15480"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HYJ42194.1"
                     /translation="MLFVDRDGTLIEEPPDEQVDSIDKIRFMPGVFAALRQAQRAGYQ
                     LAMVTNQDGLGSETFPQTHFDIPHQFMLDAFSSQGVEFDAVFVCPHRKTDGCDCRKPK
                     TKLVESFVRERKVDLAASAMIGDRETDMEFARNLGVRGLLVRRHGNKWETWHSILREL
                     TERSATIRRTTKETDIHVTVNLDNTAPITIATGIGFFDHMLEQIAKHGGFSLELKCHG
                     DLDIDEHHTVEDCALALGETLRAALGDKVGIARYGFVLPMDEAQVRVAIDLSGRAYGV
                     FEGKFAREAVGGLPTELVPHFFKSLAESLKAAVHVTIVGENTHHMIEACFKGVGRTLR
                     QAFRREGEELPSTKGVL"
     gene            2483..3082
                     /gene="hisH"
                     /locus_tag="VEW08_15485"
     CDS             2483..3082
                     /gene="hisH"
                     /locus_tag="VEW08_15485"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HYJ42195.1"
                     /translation="MSTPVAIIDSGGANIASLRAALSRLGADSVVTSDPGVIQRAARV
                     LLPGVGSAHNAMARLRGTGLDKLIPTLKQPLLGICLGMQILFETSEEGPANGLAVIPG
                     NVARLQFAPGLPVPHMGWNQLSQAKPDPLLDGVSSLDYVYFVHGYAVPAGNWTVATTE
                     YGSHFTAVARRDNFCGTQFHPERSGVVGARILANFLKAS"
     gene            3088..3864
                     /locus_tag="VEW08_15490"
     CDS             3088..3864
                     /locus_tag="VEW08_15490"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HYJ42196.1"
                     /translation="MILIPSIDLRGGRCVRLLKGNFDAETRYDLEPHELLLRYRALGA
                     NWLHVVDLDGAKDGRLANRSVIVRLASQRALNIQVGGGVRSQAVVDDLLRNGIDRVIV
                     GSAAVETPAEVQGWLKRYGAEKIGLAFDIRHDAEGIPRVLTRGWTKESKLTLWEAIES
                     YLPHGLKHVLCTDAELDGALQGPAVALYAEGVKRYPQIAWQASGGVRSAADLTALAAV
                     GSAAAISGKALLEEMIDPKELGPFLPNASSPASTSATARS"
     gene            3870..>4216
                     /locus_tag="VEW08_15495"
     CDS             3870..>4216
                     /locus_tag="VEW08_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014998871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HYJ42197.1"
                     /translation="MRFRDHQIVGDILVLAERYRNEGADEVVFYDITASPEGRSVDRN
                     WVRRVADVLDIPFCVAGGIRSVEDAEAVLNAGAEKISINSPAITNPGLIEELAARFGS
                     QCVVIGIDSASLP"
BASE COUNT          755 a         1384 c         1323 g          754 t
ORIGIN      
        1 tgccgacctc gaagccgccg tcggcgtgtc caacgaatac gcgcccgaac acctgatcat
       61 ccagactcgc gatgcgcgtg cgttgctcgc gcaggtgtcc tgcgcgggct cggtgttcct
      121 cggcgcatgg tcgccggagt ccatgggcga ctactgctcg ggcaccaacc atgttctgcc
      181 gacgtttggc tacgcgcgca gctacagtgg cgtgtcgttg ctcgactatc aaaagcgaat
      241 caccgtgcag gaactcagcg ccgatggatt gcgcgccctc ggccccaccg ccatcacgct
      301 ctcgggcatg gaaggactcg acgcgcacgg caatgcggtg aaggtccggc tggaatcgtt
      361 ggccggaggt gccgcgtgac cgacattctc gcactggcgc gcccggacat catcgcgctc
      421 aaggcctatt cgcacgccag ctgggatccg gcgttcgaac gcctgcatgc caacgaacta
      481 ccgtggcggg ctgaaaccga tcgctcgatc gcaggcctca atcgctatcc ggaaccacat
      541 ccgcacgcgc tggcgcagcg cctcgccgat ctttatggag tgccggacgg gaagttgttg
      601 cccggtcgcg gcagtgacga atccatcgac ctgctggtgc gcggcttctg ccgtgccggc
      661 gtggacaacg tcgtcatctg cccgccgact ttcggcatgt atgccgtggc ggcgcgcatc
      721 cagggcgcgg aagtgcgcga agtgccgctg ctgcccgagc gcggattcgc gctcgacacc
      781 gcacatgtgc tcgcggcctg tgatgccaac acaaaaatcg tattcctctg cagccctaac
      841 aatcccaccg gcaatgcgat ggagcccgcc gccatcgagc aattgctggc gacgctcgcg
      901 aaccgctcgc tggtggtggt cgacgaggca tacatcgagt tttcgggtga tgcttcgttg
      961 gcgaccgcac tgactcattt tccgaacctg gtcgtgctgc gcacgttgtc gaaggcgttc
     1021 gggttggccg gcgcgcgcgt gggttcgctg attgccgcgc gtgaaatcgt atcgctgctg
     1081 gccaaggtga ttccgcccta ttccatcccg cagctcacga tcgaggccgt gcttgccacg
     1141 ctcgaaggtc cgcagctcgc cattcaacgt gaacgcgtgg cgcagatccg cgccgaacgc
     1201 gaacggcttc gcgcggcgct tgcaaactgc gggtccgtac agaagatatg gcccagcgcc
     1261 gcgaatttcc tcctcgtcga tttcgcggat gctgaagtca tcctcggcgc ggcgcgtgcg
     1321 gcgaagctgc tgatccgcga catgcgcaac gtttcgccgc actcgctgcg catctcggtg
     1381 ggcagccctg aacagaacga tcgcttgatc cggagtattc cttgaccaaa gtgctgttcg
     1441 tcgaccgcga tggaacgctt atcgaagagc cgcctgacga acaggtggac tccatcgata
     1501 agatccgctt catgcccggc gtattcgccg cgctgcgcca ggcacaacgc gcgggctatc
     1561 agctggccat ggtcacgaat caggacgggc tcggcagcga gacctttccg cagacgcatt
     1621 tcgacattcc tcaccagttc atgctcgatg cgttcagctc gcaaggcgtg gagttcgacg
     1681 cggtgttcgt ctgcccgcat cgcaagaccg acggctgcga ctgccgcaaa cccaagacga
     1741 aactcgtcga gagcttcgtg cgcgagcgca aagtcgatct tgcggccagc gccatgatcg
     1801 gcgatcgcga aaccgacatg gagttcgcgc ggaacctggg cgtgcgcgga ttgctggtgc
     1861 gccgccacgg caacaagtgg gagacctggc actcgatcct gcgcgaactc acggagcgca
     1921 gcgcgacgat ccggcggacg acgaaggaaa ccgacatcca cgtcacggtg aatctcgaca
     1981 acactgcgcc gatcaccatt gcgaccggca tcggcttctt cgatcacatg ctcgaacaga
     2041 tcgccaaaca cggtggcttt tcgctcgaac tcaagtgcca cggagatctg gacatcgacg
     2101 agcaccacac ggtcgaagac tgcgcgctgg cactcggtga aacgctgcgc gcggcgctgg
     2161 gcgacaaggt cggcatcgcc cgctatggct tcgtgctgcc catggacgag gcgcaggtgc
     2221 gcgtcgccat cgatctttcg gggcgcgcgt atggcgtgtt cgaaggcaag tttgcacgcg
     2281 aagccgtcgg tggactgcct accgaactgg tgccgcattt cttcaagtcg ctggccgaaa
     2341 gcctcaaggc ggcggtccac gtcaccatag tcggcgagaa cacgcaccac atgatcgaag
     2401 cctgcttcaa gggagttgga cgcacgctgc gccaggcgtt ccgccgcgaa ggcgaagagc
     2461 tgccctcgac caagggagtg ctgtgagcac gccggttgcg atcatcgaca gtggtggcgc
     2521 caacatcgcc tcattgcgcg cggcgctttc gcggctcggc gcggattcgg tcgttaccag
     2581 cgatcccggc gtcatccagc gtgccgcgcg cgtgctgctg cccggcgtgg gctcggcgca
     2641 caacgccatg gcccgcctgc gcggcaccgg tctcgacaag ctgattccta cgctcaagca
     2701 gcctttgtta ggcatctgcc tcggcatgca gatcctgttc gagacttccg aggaaggacc
     2761 cgccaacggg cttgcggtca ttccgggcaa cgtggcccgt cttcagttcg cacccggcct
     2821 gccggtgccg cacatgggct ggaaccagct cagccaggcc aaacccgatc cgctgctcga
     2881 tggcgtgagc tcgctcgact atgtgtattt cgtgcacgga tacgcggtgc ccgcaggcaa
     2941 ctggacggtc gccaccaccg agtacggcag ccattttacg gctgtagccc gccgcgacaa
     3001 tttctgtggc acgcagtttc accccgagcg gtcgggcgtg gtcggcgcgc gcatcctcgc
     3061 caatttcctg aaagcaagct gagaatcatg atcctaattc catcgatcga cttgcgcggc
     3121 ggccgctgcg tgcgcctgct caagggcaac ttcgacgcgg aaacgcgtta cgacctcgag
     3181 ccgcatgaac tgctgttgcg ttatcgcgcg ctcggtgcga actggctgca cgtcgtcgat
     3241 ctcgacggcg ccaaagacgg ccggctcgcc aatcgcagcg tcatcgtgcg gctcgcttcg
     3301 cagcgcgcgc tgaacatcca ggtgggtggc ggcgtgcggt cccaggcggt cgtcgacgac
     3361 cttctgcgca acggcatcga ccgcgtcatc gtcggcagcg cggccgtgga aacgccggcc
     3421 gaggtgcaag gctggctcaa gcgttacggt gccgagaaaa tcgggctggc attcgacatc
     3481 cgccatgatg ccgaaggcat cccgcgcgta ttgacgcgcg gctggacgaa agagtcgaag
     3541 ctcacgctct gggaagccat cgaatcctat ctaccccacg gcctgaagca cgtgttgtgc
     3601 accgacgccg agctcgacgg tgccctgcag ggcccggcgg tggcgctgta tgccgaaggc
     3661 gtgaagcgtt atccacagat cgcctggcag gcttccggcg gagtccgctc cgcagccgat
     3721 ctcacggcac tcgcggccgt gggaagcgcc gccgccatca gcggcaaggc gttgctcgaa
     3781 gaaatgatcg atccgaagga gttaggccca ttcttgccaa acgcatcatc ccctgcctcg
     3841 acgtccgcga cggccaggtc gtaaagggaa ttcgcttccg cgatcaccag atcgtgggcg
     3901 acatcctggt gctcgccgag cgctatcgca acgaaggcgc ggatgaagtc gtgttctacg
     3961 acatcacggc gagccccgaa ggccgcagcg tcgaccgcaa ctgggtccgg cgcgtggcgg
     4021 acgtgctcga tatcccgttc tgcgtggcgg gcggcattcg ttcggtggaa gacgccgaag
     4081 ccgtgctcaa tgcgggcgcc gagaagatct ccatcaactc cccggccatc accaaccccg
     4141 gcctcatcga agaacttgcc gcccgattcg gttcgcagtg cgtggtcatt ggcatcgaca
     4201 gcgccagcct tcccga
//
LOCUS       DASQJS010000395         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4029622, whole genome shotgun sequence.
ACCESSION   DASQJS010000395 DASQJS010000000
VERSION     DASQJS010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4029622"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VEW08_15500"
     CDS             <1..924
                     /locus_tag="VEW08_15500"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HYJ42198.1"
                     /translation="LAADLSVTRAAVWKAVEQLRELGVALDAQTNKGYRLAPGVSALS
                     VERIQSLLSADVRARLEVLLVDWTLESTNTRLLEALPPAAGTAAVVLAEHQTGGRGRR
                     GRGWVAPPGGAICLSLGWQYADMPADLSALSLIVGLSAVNALTALGVPGVMLKWPNDL
                     VTGEGKLGGILIEMRAESGGPVHVVAGIGLNVALDDDARATVKATGNTADDIRAHCAP
                     APDRNTIVAALLARLIPAFEGFPRHGLAPHLENWHEFDALRDREVRVENAGEISRGVA
                     RGVDAHGALLVETPAGVRRFISGEVTVRIDT"
     gene            921..1679
                     /locus_tag="VEW08_15505"
     CDS             921..1679
                     /locus_tag="VEW08_15505"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HYJ42199.1"
                     /translation="MTTTTLLVDIGNTRVKWATLRGLAQGRMQAAAHEDSGLALRALV
                     REAPRDVSRVMVVSVVDEALSRVLDAAARRRFGVIPEYVASTRSACGVTNGYRETWRL
                     GADRWVSAIGAHSLSGGRAAVIANVGTALTIDAVTGGGRHRGGAIIPGPKVMVESLLA
                     GTHGIRRRAQGGDDSVRSLFATDTASALAAGSRYAAAAFIDRAVAEAARTFRSRPVLF
                     LTGGAAPMLKRHVESRARVVPDLVLRGLAVFARA"
     gene            1713..2351
                     /locus_tag="VEW08_15510"
     CDS             1713..2351
                     /locus_tag="VEW08_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42200.1"
                     /translation="MRAALLLLLLANLAFFAWMQWLAPGEAQLPISPKVNAPPLELVN
                     EARPATGGDGSCVTVGPFATNELAARARQTLNDSGYPSIPREVATREFDGYWVYLESP
                     PTETGERRLLDRLKKGGIADAQALGEPGSRRISLGVFSDETRAAAQSEKVARLQLLPQ
                     IEGRQKTGTAIWLDLTLKSDSPPLEGQKFPAGDTELEFRACEAPAAAAPVTQ"
     gene            2408..2483
                     /locus_tag="VEW08_15515"
     tRNA            2408..2483
                     /locus_tag="VEW08_15515"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2441..2443,aa:Thr,seq:tgt)
     gene            complement(2513..3568)
                     /locus_tag="VEW08_15520"
     CDS             complement(2513..3568)
                     /locus_tag="VEW08_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003146062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HYJ42201.1"
                     /translation="MHSLTPEYLARLRFDSNQAATLRAIGEYRGKQALFYRQAPEVLK
                     GLRDIAVIESSESSNRLEGVIVAPNRLKALILQNTKPRDRSEQEVAGYRDALALIHES
                     AREMRFTPNVILQLHSTLYRFMPAPGGRWKAADNEIIEKQPDGKVRIRFKATPAHLTP
                     IAMEQLCAGYSAAVDTTQHDPLIAVPLAILDFLCIHPFTDGNGRVGRLLTLMLLYHFE
                     YEVGRYISIERVYEATKESYYETLEASSQGWHESRHDPYPWLNYFWGVLLRAYREFEE
                     RVGEVRSGRGAKTEQVREAVLARNMPFSISDIEAACAGVSRDTVRLVLREMKKEGLIA
                     STGKGRSAKWIKLKEGN"
BASE COUNT          624 a         1192 c         1176 g          687 t
ORIGIN      
        1 ctggcggcgg acctgtcggt gacgcgcgcc gccgtctgga aagcggtcga gcaattgcgc
       61 gagctcggcg tggcgctgga cgcgcagacc aacaagggtt atcggctcgc gccaggcgtc
      121 agcgcgttgt cggtggagcg aatccagtcc ctgttgtccg ccgacgtgcg cgcacgtctc
      181 gaagtgctgc tggtggactg gaccctcgaa tccaccaata cccggttgtt agaggcactg
      241 ccgccggcgg caggtaccgc cgcggtggtg ctggccgaac accagaccgg cggacgtggc
      301 cggcgcgggc gcggctgggt cgcgccgccc ggaggcgcca tctgcctgtc gctcgggtgg
      361 caatacgcgg acatgcccgc ggatctgtcc gcgctgtcat tgatagtggg tttgagcgca
      421 gtgaatgcgt tgaccgcgct cggcgtgccc ggcgtcatgt tgaaatggcc caacgacctg
      481 gtgacaggcg agggcaagct cggtggcatt ctcatcgaga tgcgcgccga atccggcggg
      541 cccgtgcacg tcgtcgcagg gatcggcctc aacgtcgcgc tcgatgacga cgctcgcgcc
      601 accgtgaaag ccaccggcaa caccgccgat gacattcgcg cgcactgcgc gcccgcgcca
      661 gatcgcaaca ccatcgttgc cgcactgctc gcgcgcctga ttcccgcgtt tgaaggattc
      721 ccgcgccacg gcctcgcgcc acacctggaa aactggcacg agttcgacgc actgcgcgat
      781 cgcgaagtgc gcgtggaaaa cgccggcgaa atctcgcgcg gcgtggcgcg cggcgtcgac
      841 gcccatggcg ccctgctggt cgaaaccccc gccggcgtgc gacgcttcat ctccggcgaa
      901 gtcacggtgc gcatcgatac atgacaacta caaccttgtt agtcgacatc ggcaataccc
      961 gcgtcaagtg ggccacgctg cgcggccttg cgcaggggcg catgcaggcg gccgcgcacg
     1021 aagactccgg tctcgcgctg cgcgcgctgg tacgcgaagc gccgcgcgac gtcagccgcg
     1081 tcatggttgt gagcgtcgtg gacgaagcgc tgtcccgcgt gctcgatgcg gccgcgcgcc
     1141 ggcgtttcgg agtcattccc gaatacgtcg cttccacgcg aagcgcctgc ggcgtcacca
     1201 acggttatcg cgaaacctgg aggctcggtg cggatcgctg ggtgtcggcg atcggcgcgc
     1261 actcgctgtc gggtggccgc gccgccgtca tcgccaacgt gggaaccgcg ctcaccatcg
     1321 atgccgtcac cggcggcggc cgccatcgtg gcggcgccat cattccaggc cccaaggtca
     1381 tggtggaaag cctcctggcc ggcacccacg gcattcgccg ccgcgcccag ggcggtgacg
     1441 actcggttcg ttcgctgttc gccaccgaca cggcgagcgc gctcgccgcg ggttcgcggt
     1501 acgccgccgc ggcattcatc gaccgagcag tcgccgaggc ggcgcgcacg tttcgcagcc
     1561 ggcccgttct gttcctgacc ggcggcgccg cgccgatgct caagcgccac gttgaaagcc
     1621 gtgcgcgcgt ggtgccggat ctggtgcttc gcggcctcgc cgtgttcgcg agggcttgag
     1681 gcgtaaccgc atttgctaca ctcgcgcctc gcgtgcgtgc agccttactc cttctcttat
     1741 tagcgaatct cgcgtttttc gcgtggatgc agtggcttgc gcccggcgaa gcgcagttgc
     1801 ccatctcgcc gaaggtgaat gcaccgcccc tcgaactggt caatgaggcc aggcccgcaa
     1861 ctggcggtga tggcagctgt gtcaccgtgg gcccgttcgc caccaacgaa ctggcggccc
     1921 gcgcgcggca gacgttgaat gacagcggct atccgtcgat accccgcgaa gtcgcaaccc
     1981 gggagttcga cggctactgg gtctatctcg aaagtccccc gacggaaacc ggcgagcggc
     2041 gattgctcga tcgcctgaaa aaaggtggca tcgccgacgc gcaagccctg ggcgagcccg
     2101 gttcgcgccg aatctcgctc ggcgtgttct cggacgagac ccgggccgcg gcgcagtccg
     2161 agaaggtcgc caggctgcag ttgctgccgc agatcgaagg ccggcaaaag accggtaccg
     2221 ccatctggct ggatctgacc ctgaaatcgg actcgccacc gctcgagggc cagaaattcc
     2281 cggccgggga cacggaactc gaattccgcg cctgcgaggc gccggcggcc gctgccccgg
     2341 tcacgcaata gcgcgcggcg cgttgcgcgc ctaaaatcgc gccttctcga atcgtttgat
     2401 tttcaaggcc ggcttagctc agttggtaga gcacctgatt tgtaatcagg gggtcgcggg
     2461 ttcgatccct gcagccggca ccaaactcca gtctcgtcat cccatgatcg attcagtttc
     2521 cttccttcag cttgatccac tttgcggatc gtcccttgcc ggtcgaggcg atcaaccctt
     2581 ctttcttcat ctctcgcagg actagccgca cggtgtcgcg ggacacgccg gcgcacgcag
     2641 cctcgatgtc ggagatcgaa aacggcatgt ttcgcgcgag caccgcttcg cgcacctgtt
     2701 ctgtctttgc accgcgaccg ctgcgcactt caccgacgcg ttcttcaaac tcgcgatacg
     2761 cgcgcagcag tacaccccaa aaataattca accacggata gggatcgtgg cggctctcgt
     2821 gccatccctg cgaactcgct tcgagcgttt cgtagtagct ctctttggtg gcctcgtaga
     2881 cacgttcgat gctgatgtag cgacccactt cgtattcgaa gtggtaaagc aacatcagag
     2941 tcaacagacg cccgacgcgc ccgttgccat cggtgaacgg atgaatgcag aggaaatcga
     3001 gaatcgcgag tggcacagcg atcagtggat cgtgctgggt tgtgtcaacg gcggcggagt
     3061 aacccgcgca aagttgctcc atcgcgatgg gagtcagatg cgcaggcgtt gctttgaaac
     3121 gaatgcgcac ctttccgtct ggttgctttt cgatgatctc gttgtccgcc gctttccacc
     3181 gccctcctgg cgctggcatg aaccgataca gcgtcgagtg cagttgcagg atcacatttg
     3241 gagtgaaacg catctcgcgc gccgactcgt ggatcaacgc caaagcatcg cgatagcccg
     3301 ccacttcctg ctcggaacga tctcgcggtt tggtgttctg gaggatcagg gccttgaggc
     3361 gatttggcgc tacgatcacc ccttcgaggc ggttcgatga ttcgctcgac tcgatcacgg
     3421 cgatatcgcg tagccctttc agcacttccg gggcctggcg gtagaaaagc gcttgtttgc
     3481 ctcggtattc gccgatggcg cgcagcgtcg ccgcctggtt cgagtcgaac cggagacggg
     3541 cgaggtattc gggtgtcagg gagtgcaact ggagccccag attcaggaat tggggcgatc
     3601 gtagtctata aatagggtaa tgagaacttg aataccctgt ttatgggtgg ccgttacccc
     3661 attcaaatgc gcgttcgaa
//
LOCUS       DASQJS010000396         6492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3711829, whole genome shotgun sequence.
ACCESSION   DASQJS010000396 DASQJS010000000
VERSION     DASQJS010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3711829"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VEW08_15525"
     CDS             <1..274
                     /locus_tag="VEW08_15525"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HYJ42202.1"
                     /translation="PWEAALGATVEVPTLSGPVSLKVPAGTSAAKKLRLAGRGLPKPR
                     GGAGDLIAVAQIVVPGEMGERERELYQQLADVSEFNPRRHFAAEGT"
     gene            271..585
                     /locus_tag="VEW08_15530"
     CDS             271..585
                     /locus_tag="VEW08_15530"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HYJ42203.1"
                     /translation="MNIEAEVLWLDEHREVSLRELIDLSGLREAELLELVHSGVIPAN
                     DRPGAGYHFSARVVTVARTACRLRDDFELDTRGLSVALRLLERVRALEEEIGRLRAEL
                     PR"
     gene            634..2136
                     /locus_tag="VEW08_15535"
     CDS             634..2136
                     /locus_tag="VEW08_15535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYJ42204.1"
                     /translation="MFMKRRQFIHSALAAIPAVAMPRFASAADATAPLPDVEALSLHG
                     RQIMLKGSDVKDFAARLRGDLMLRDSAGYDSARRAWNGAFDRHPALIARCSGPADVME
                     AVKFAASSQLLLSVRGGGHSLSGQSVCEKGLMIDLSQMNSARVDPARRIATIEGGALL
                     GALDREALVHGLATTAGTVSHTGVGGLTLGGGFGRLGRKFGLTCDNVRAIDVVTAGGK
                     FITASAHQNKDLFWGLRGGGGNFGIATSFEFQLHPVDPIMLGGELTYSFEDAPKVLEF
                     FFGFAANAPDELNLDLSVVRLPNDMRFMSVDVCYCGSIAEGEKALAPLRQIRKPIKDT
                     VVPTPYVTLQASGDEGTAHGHKYYIKGGFVQKTTPALIDAILATVAAANLPTVQAVVL
                     PQGGGAYARVKPGVTAFAQRAAPHNMFVFSRWDDPAMSAAVGNWARGAWKSLEPLTQG
                     FYVNEFNDDGDRMKATYGVNYDRLVALKTKVDPDNLFRLNANVAPKGAQA"
     gene            complement(2184..2609)
                     /locus_tag="VEW08_15540"
     CDS             complement(2184..2609)
                     /locus_tag="VEW08_15540"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HYJ42205.1"
                     /translation="MKNLIEAAADIGNFKTLLSAFKSASLIDMLRSPGQYTIFAPTDA
                     AFKRLPAGSLDALLKNARTLKPFLCNHIMAGVKAANDILPGELKPMEGRALRASLHGS
                     AIHINGATIVQSDIRATNGLIHAIDDVLVPLNPILAAVA"
     gene            2766..4190
                     /locus_tag="VEW08_15545"
     CDS             2766..4190
                     /locus_tag="VEW08_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HYJ42206.1"
                     /translation="MFHDADALLLARIQFGFTVSFHFIFPAFSIGLASFLAVLEALWL
                     KTGKALYLDLFKYWLKIFAVVFAMGVVSGIVMSYQFGSNWAVFSDKAGPIVGPLMAYE
                     VLTAFFLEAGFLGVMLFGMQKVGRRLHFFATLMVAVGTFISAFWILSVNSWMQTPTGY
                     AINADGQFVPAAGWAAIIFNPSFPYRLVHTVIAAYLTTALAVGAVGAWHLLKDSRQEH
                     ARKMFSMAMWMAALVAPVQIFVGDLHGLNTLEHQPAKVMAMEGHYQSHPDGAPLILFG
                     IPNDEEQRMDHAIEIPRLSSLILKHDLDAPLKGLDTIEPEDRPPVEVVFWTFRVMVAL
                     GSLMLLLGICSLLARAMRRLYHWRWLLRFALAMGPAGFIAVIAGWMTTEVGRQPFTIY
                     GLMRTADSVSPLAAPAVATSLIAFIVVYFAVFGMGTWYLLRLMSHAPHSHEPDLPNAP
                     AHAAGITPGPAMAAAGAPKEVPHG"
     gene            4183..5190
                     /gene="cydB"
                     /locus_tag="VEW08_15550"
     CDS             4183..5190
                     /gene="cydB"
                     /locus_tag="VEW08_15550"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HYJ42207.1"
                     /translation="MDDSTLLTNVWAFILAFAVFMYVVMDGFDLGIGILFPVFGVGRE
                     RDTAMNSIAPVWDGNETWLVLGGGGLMAAFPLAYGVIMSALYPPLIAMLLALVFRGVA
                     FEFRWRDPSHRHIWDFAFTAGSFVAAFTQGIMLGALLQGINVAGRAYGGGWWDWLTPF
                     SLLTGVSVVAGYSLLGACWLNWKTDGETQKHARRLMRILAPITLGAIGAVSVATLLLG
                     DEYVKRWFAMPAVLVTAQVPLIVAILTWRFARSLRRNYELAPFVITLAVFGLSFVGLG
                     ISMYPYLIPNSVTLQMAAAPPSSQAFMLVGASVLVPLILAYTAWSYWVFRGKVAHEGY
                     H"
     gene            5193..5309
                     /locus_tag="VEW08_15555"
     CDS             5193..5309
                     /locus_tag="VEW08_15555"
                     /inference="COORDINATES: protein motif:HMM:NF022085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2474 family protein"
                     /protein_id="HYJ42208.1"
                     /translation="MRPEEAPLVRRLGWMALIWAISVTLLGVVSLILRWWLK"
     gene            5333..6064
                     /locus_tag="VEW08_15560"
     CDS             5333..6064
                     /locus_tag="VEW08_15560"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HYJ42209.1"
                     /translation="MNPHALVYAAGAILLGAVGICFHEFAMQWQPVPAGIGMRAQLAY
                     LAGALLAIGGVLLLVPKLERAGALLLTVFIGLWMLVLNLPLAIASASHIGAWNSPAEI
                     TFMASGALALFASGIKGNARPTALLAARLLAGASAMVFGFAHFNYIDFTASMVPGWIP
                     YKTFWAWATGAGHLAAGLALVSGIRARLGAGCEAAMMGSFVVLLHLPRVIAAPDQHVE
                     WIMLGVSGMLTGAALLIRKYATIGA"
     gene            6061..6303
                     /locus_tag="VEW08_15565"
     CDS             6061..6303
                     /locus_tag="VEW08_15565"
                     /inference="COORDINATES: protein motif:HMM:NF018500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HYJ42210.1"
                     /translation="MSEEEDSKPVEIPHTELAPETLRAVIESFVLREGTDYGAQDVPF
                     DRKVADVKRQLERREAVIVYDPGSDSVDIVVRRPGG"
BASE COUNT         1055 a         2000 c         2162 g         1275 t
ORIGIN      
        1 gccgtgggaa gcggcgctgg gcgcgacggt ggaagtcccg accctgtccg ggccagtgag
       61 cctcaaggtg ccggcgggca cttcggcggc gaagaagctg cggctcgcag gccgggggct
      121 gccgaaaccg cgcggtggcg cgggcgatct cattgcagtg gcgcagatcg tcgtgcccgg
      181 cgagatgggc gaacgcgagc gcgagctcta tcagcagctc gcggatgtca gtgaattcaa
      241 tccgcgccgg catttcgccg cggagggcac atgaacatcg aagcggaagt gctgtggctc
      301 gacgaacacc gcgaggtcag cctgcgtgaa ctcatcgacc tgtcgggact gcgtgaagcg
      361 gagctgctcg agctggttca cagtggcgtg ataccggcga acgaccggcc cggtgcgggc
      421 tatcacttca gcgcgcgcgt ggtcaccgtg gcgcgtaccg cctgtcggct ccgcgatgac
      481 ttcgagctgg atacgcgggg cctgtccgtg gcgctgcgat tgctcgagcg cgtgcgcgcg
      541 ctcgaagagg aaatcgggcg gttgcgcgcc gagctgcccc ggtagggttt ggggcgtaga
      601 gttgtcaccg aataaacaaa acaagaaggt tccatgttca tgaaacgccg gcaattcatt
      661 cactcggcgc tagcggccat tcctgcagtc gccatgccga ggttcgcgtc cgcagccgac
      721 gccacggcgc cgctgcccga cgtcgaagcg ctgtccctgc acggcaggca gatcatgctg
      781 aagggttcgg acgtgaaaga tttcgcggcg cggttgcgcg gcgatctgat gctgcgcgac
      841 agcgcgggtt atgactcggc gcggcgcgcc tggaatggtg cgttcgatcg gcacccggcg
      901 ttgatcgcgc gatgcagtgg gccggccgat gtgatggagg cggtgaagtt cgcggcgtcc
      961 agccagttgc tgttgtcggt gcgcggtggt ggacacagcc tctcgggcca gtcggtatgc
     1021 gaaaagggcc tgatgatcga cctgtcgcaa atgaacagcg cgcgtgtcga tcccgcgcgc
     1081 cgcatcgcca ccatcgaagg cggcgccttg ttaggcgcgc tcgaccgcga ggcactcgtg
     1141 catggtctcg caacgacggc gggtacggtt tcgcacaccg gcgtgggtgg gcttacgctc
     1201 ggtggaggct tcggccgcct cggccggaag tttggcctga cctgcgacaa cgttcgcgcc
     1261 atcgacgtgg tgactgctgg cggaaaattc atcactgcca gcgctcacca gaacaaggat
     1321 cttttctggg gactgcgcgg cggcggtggc aacttcggga tcgccacttc gttcgagttc
     1381 cagttgcatc ccgtcgatcc catcatgctg ggcggtgagc tcacgtactc gttcgaggac
     1441 gcacccaaag tgctggagtt tttcttcggg tttgcggcga atgcgccgga cgagctgaat
     1501 ctcgacctgt ccgtcgttcg cctgccgaac gacatgcgct tcatgtccgt cgacgtctgt
     1561 tactgcgggt ccattgccga aggcgaaaag gcgcttgcgc cattgcggca gattcgcaag
     1621 ccgatcaaag acaccgtggt gcccacgccc tacgtgacgc tgcaggcttc gggagatgaa
     1681 ggtactgcgc atggccacaa gtactacatc aagggcggtt tcgtgcagaa gaccacgccg
     1741 gcgttgatcg acgcgatcct cgcgacggtt gccgcggcga acctgccgac ggtgcaggcg
     1801 gtggtgctgc cgcaaggcgg cggcgcctat gcgcgcgtga aacccggcgt caccgcattt
     1861 gcgcagcgcg ccgctccgca caacatgttc gtgttcagcc gctgggacga tccggcaatg
     1921 agcgccgctg tgggcaactg ggcgcgcggc gcctggaaat ccctggagcc gctgacccag
     1981 ggcttttacg tcaacgagtt caacgacgat ggcgaccgca tgaaggccac gtacggcgtc
     2041 aactacgacc ggctggtcgc gctgaagacg aaggtcgacc ctgacaacct gttccggctg
     2101 aacgcgaacg tggcgccgaa gggcgcccag gcttgaaagc accgaagggc gccccggctt
     2161 gaaagcgccg aagggcgccc ggttcacgcc accgcggcca gaatcggatt gagcggcacc
     2221 agcacgtcgt cgatggcgtg gatgaggcca ttggtcgcgc ggatgtcgga ctgcacgatc
     2281 gtcgcgccgt tgatgtgaat ggcactgcca tgcagcgagg cacgcagcgc tcggccttcc
     2341 atgggcttga gttcgcccgg caggatgtcg ttggcggctt tgacgcccgc catgatgtgg
     2401 ttgcacagaa acggcttgag cgtacgcgcg ttcttcaaca acgcatcgag cgaaccggcg
     2461 ggcaggcgtt tgaaggcggc atccgtgggc gcgaagatgg tgtactgacc cggcgaacgc
     2521 agcatgtcga tgagcgatgc cgatttgaac gcgctgagaa gtgtcttgaa gttaccgatg
     2581 tccgctgccg cttcgatgag atttttcata gatccttgag tttgtttctg tgtttatcga
     2641 gccagtatgc gcggttaagg cagtcggaac cggcggcagc ggaaagatat ccatatcgac
     2701 ccgccggcca ccgggacgag tgatttgtgc gccgtaggcg cctcgctcca gtctaaaatg
     2761 caggcatgtt tcacgacgcc gacgcgctac ttctcgcccg catccagttc ggattcacgg
     2821 tcagctttca cttcattttc ccggcgttct cgatcgggct ggcaagcttt ctcgcggtgc
     2881 tcgaagcgct gtggctgaag accggcaagg cgctgtatct cgaccttttc aagtactggc
     2941 tgaagatctt cgccgtggtg ttcgccatgg gcgtggtatc cggcatcgtc atgtcgtacc
     3001 agttcggcag caactgggcg gtgttctccg acaaggcggg cccgatcgtc gggccgctga
     3061 tggcctacga agtcctcacc gcctttttcc tcgaagccgg ttttctcggc gtgatgctct
     3121 tcggcatgca gaaagtaggc aggcgcctgc attttttcgc gacgctcatg gtggccgtgg
     3181 gcaccttcat ctcggcgttc tggatcctga gcgtgaacag ctggatgcag acgcccaccg
     3241 gatatgccat caacgccgac ggacagttcg tgccggccgc gggctgggcg gccatcatct
     3301 tcaacccaag cttcccctat cgcctcgtgc acacggtgat cgcggcctat ctaaccaccg
     3361 cgttggcagt cggcgccgtg ggcgcgtggc atctgctgaa ggattcgcgc caggaacacg
     3421 cgcgcaagat gttctccatg gccatgtgga tggccgcgct ggtcgcgccg gtgcagatct
     3481 ttgtcggtga cctgcacggc ctcaatacgc tcgagcacca gccggcgaag gtcatggcga
     3541 tggaagggca ctaccagagt caccccgacg gtgcgcccct cattctcttc ggcatcccga
     3601 acgacgaaga gcagcgcatg gatcacgcca tcgaaattcc gcggctgtcc tcgctgatcc
     3661 tcaagcacga tctcgacgcg ccactgaagg gcctcgacac gattgagcct gaggatcgcc
     3721 cgcccgtgga agtggtcttc tggacgttcc gcgtgatggt ggcgctcggc tcccttatgc
     3781 tgttgttagg catctgcagc ctgctcgcgc gggcaatgcg caggctgtac cactggcgct
     3841 ggctgctgcg cttcgcgctc gccatgggcc cggcgggatt catcgccgtc atcgccggct
     3901 ggatgaccac cgaagtcggc cggcaacctt tcacgattta cggcttgatg cgcacggcgg
     3961 attcggtgtc gccgctggct gcgcccgcgg tcgccacttc gctcatcgcc ttcatcgtcg
     4021 tgtacttcgc ggtgttcggc atgggtacgt ggtacctgct caggctcatg tcgcatgcgc
     4081 ctcattccca cgaaccggac ctgcccaatg cgcccgcgca tgcggcgggc atcacaccgg
     4141 gaccggcgat ggccgcggcc ggcgcgccga aagaggtgcc ccatggatga cagcactttg
     4201 ctcaccaacg tctgggcgtt catcctggcg ttcgccgtgt tcatgtacgt ggtcatggac
     4261 ggcttcgatc tcggcatcgg catcctgttt ccggtgttcg gtgtgggccg cgagcgcgac
     4321 acagccatga actcgatcgc gccggtatgg gacggtaacg agacctggct ggtgctgggc
     4381 ggaggcgggt tgatggcggc gtttccgctg gcctacggcg tcatcatgtc ggcgctgtat
     4441 ccgccgttga tcgccatgct gctggccctc gtattccgcg gtgtggcctt cgagtttcgc
     4501 tggcgcgatc cgtcgcaccg gcacatctgg gatttcgcct tcacggcagg gtcgttcgtt
     4561 gccgcattca cgcagggcat catgctcggc gcactgctgc aggggatcaa tgtggccggt
     4621 cgcgcgtacg gcggtggctg gtgggactgg ctcacgccat tcagcctgct gaccggcgtg
     4681 agcgtcgtcg ccggatattc tttgttaggc gcctgctggc tcaactggaa gaccgatggc
     4741 gagacgcaga aacacgcgcg ccggttgatg cgcatcctcg cgcccatcac gctcggcgcg
     4801 ataggcgcgg tgagcgtggc gaccttgctc ctcggtgacg agtacgtgaa gcgctggttc
     4861 gcgatgccgg ccgtactcgt gaccgcgcag gtgccgctca tcgtcgccat cctcacctgg
     4921 cgtttcgcgc gcagcctgcg gcgcaattat gaacttgcgc ctttcgtgat cacgcttgcg
     4981 gtgttcggcc tgtccttcgt cggcctcggc atcagcatgt atccgtatct catcccgaac
     5041 agcgtcacgc tgcagatggc cgcggcgccg cccagcagcc aggccttcat gctggtgggc
     5101 gcgagcgtac tcgtgccgct gatcctcgcg tacaccgcgt ggtcgtattg ggtgtttcgc
     5161 ggcaaggtgg cgcacgaggg ataccattga gcgtgcgtcc cgaagaagcg ccgctcgtgc
     5221 ggcggctcgg ctggatggcc ctcatatggg ccatcagcgt gactttgtta ggagtggtta
     5281 gcctcatcct gcgctggtgg ttaaagtagc ggcaatctca ggggagccgc tcatgaatcc
     5341 acacgcactg gtctacgccg ccggcgccat cttgttaggc gcggttggca tttgtttcca
     5401 tgaattcgcc atgcagtggc agcccgtgcc ggccggcatc ggcatgcgcg cgcaactcgc
     5461 gtacctggcc ggcgcgttgc tggccatcgg cggcgtgctg ctgctggtgc caaaactgga
     5521 acgtgccgga gctctgctgc tcaccgtgtt catcgggctg tggatgctgg tgctgaatct
     5581 tccgctcgcc atcgcgtcgg cttcgcacat cggtgcatgg aacagtccgg cggaaatcac
     5641 cttcatggcc tcgggcgcgc tggcgctgtt tgccagcggc atcaagggca acgcccggcc
     5701 gaccgcgctg ctcgctgcgc gactgctggc gggagcgagc gcaatggtct tcggcttcgc
     5761 gcacttcaac tacatcgact tcacggcatc gatggtgccg ggctggattc cctacaagac
     5821 gttctgggcc tgggccaccg gcgcaggaca tctcgcagcg ggcctggcgt tggtgagcgg
     5881 tatccgtgcg cggctgggcg ccggttgcga ggccgcgatg atgggttctt tcgtggtgct
     5941 gctgcacctg ccgcgggtca tcgccgcgcc agaccagcac gtcgagtgga tcatgctggg
     6001 tgtgtccggc atgctcaccg gcgccgcgct gctgataagg aaatacgcca caatcggggc
     6061 gtgagcgaag aagaagactc gaagcccgtc gaaattccac acaccgagct ggcgccggaa
     6121 acactgcgcg ccgtcatcga gtctttcgtg ctgcgagaag gcacggatta tggggcgcag
     6181 gatgtgccct tcgatcgcaa ggtggcggac gtcaagcgcc agctggagcg ccgcgaggcg
     6241 gtgatcgtct acgaccccgg cagcgacagc gtggatatcg tggtacggag gccgggtggt
     6301 tagctaatat gcgaacccat gtctgaccga gagatcacct ggcgcgacaa gctgcgctat
     6361 cggttcgaga acactctttc caagggcacc atcgccatca tcggctggct ggcgttgctg
     6421 tccaccgcca tcgtgatgct ggccgcgttc atcctcgcgg ccatgcgagt cggccaggac
     6481 ccggccgacc ac
//
LOCUS       DASQJS010000397         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2772945, whole genome shotgun sequence.
ACCESSION   DASQJS010000397 DASQJS010000000
VERSION     DASQJS010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2772945"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..541
                     /locus_tag="VEW08_15570"
     CDS             260..541
                     /locus_tag="VEW08_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42211.1"
                     /translation="MAETTTIRLPPKLRARLRVIAKETDRSVHSIILEAVERHTAYEE
                     QMRKLVKEALAADAEIDRTGDVYRAEDVHAWAQRLASGADHPRPKPWRK"
     gene            529..792
                     /locus_tag="VEW08_15575"
     CDS             529..792
                     /locus_tag="VEW08_15575"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYJ42212.1"
                     /translation="MAQIVYAAHALDEVERAHQPAGAAAIQSAVDNLAAHPLVGRRVE
                     DDLRELVISFGDTGYIALYRFVVQQDTVRVLALRHQREIGYLP"
     gene            complement(798..1259)
                     /locus_tag="VEW08_15580"
     CDS             complement(798..1259)
                     /locus_tag="VEW08_15580"
                     /inference="COORDINATES: protein motif:HMM:NF025703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HYJ42213.1"
                     /translation="MNATQVRALLAIAVVLVLGAVNWSIFAKERIKTQGDRIFLALAP
                     VDPRSLMQGDYMALRFELANNISAEAAGSAPIFVDARGIATLNPSPATADLRIRYRVR
                     NGQVWLGTNAYFFEEGTAARYERARYGEFRIDRDSGEAVLVGLADENLKKL"
     gene            complement(1256..2275)
                     /locus_tag="VEW08_15585"
     CDS             complement(1256..2275)
                     /locus_tag="VEW08_15585"
                     /inference="COORDINATES: protein motif:HMM:NF025709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4401 domain-containing protein"
                     /protein_id="HYJ42214.1"
                     /translation="MSDAANEFSALMRAQGVIPRDAPAPPGESADRPWFVSLLQGVAG
                     WLAGIFLLIFIGLVFKPESGPMIFGLGALLLVAAWFMYHADRHGVFLDQLALAVSIAG
                     QIAVAGSVLKDYYTGLGMCVTALVVQIFVLLVMPNKIARTLAALFATIAWIWMVRFLV
                     RPGSAEQVLFDERHAGDVAALGAWTLPVTWLLTWLPLLALAAWLILREPQWMASGLRR
                     LARPLLTGLLLGLSIGAIVGEPFTLVIWGADSWGRGIDWLAIFPLLSIGLTLFAAYGA
                     FRVRSGGLLGFAVFAALVHLSRFYYLYGTTLMWKSLIMLCAGAALLLAGVALRNRKPE
                     SGEVA"
     gene            complement(2272..>2460)
                     /locus_tag="VEW08_15590"
     CDS             complement(2272..>2460)
                     /locus_tag="VEW08_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42215.1"
                     /translation="VYPLAVVMGAFIIISLVWLAQLNGFDDEGMLLMLALWLISTSTA
                     AGRVLTVLNRRWRAEAAA"
BASE COUNT          509 a          783 c          787 g          381 t
ORIGIN      
        1 agggaaggca aagcgcggcc acgagggtgg ccaggaaact gacattaatg ggtttcatga
       61 ggttctcgag ggaggagccg gaaggatcga tggggcgcat tctatatata agagtccaaa
      121 tggggacatg gatgtgtggg gacagactta agtctgtccc cactcgttta gctggcaaat
      181 ggggccgcac ctcatggact gagacgcgcg ccgagctgtt ttggttgcac ttgcgggctt
      241 gtgctacttt cgcggcacga tggccgaaac caccacgatt cgactcccac ccaagctgcg
      301 cgcgcggctt cgcgtaatag cgaaggaaac cgatcgctcg gttcacagca tcattttgga
      361 agcggtcgaa agacacaccg cgtacgaaga gcagatgcgc aagctggtga aagaggcgct
      421 cgccgcggac gctgaaatcg accgaaccgg cgatgtgtat cgcgccgagg acgttcatgc
      481 ctgggcacag cgccttgcga gcggtgcgga ccatccgcgg ccgaagccat ggcgcaaata
      541 gtctatgcgg cgcacgcgct cgacgaagtc gagcgggcgc accagcccgc cggtgcggcg
      601 gcgattcaat ccgccgtcga caatctcgcg gcgcacccac tcgtggggcg gcgcgtcgaa
      661 gatgatctgc gcgagctggt catctctttc ggcgacacgg gatacatcgc gctctatcga
      721 ttcgtggtgc agcaagacac ggtgcgcgtg ctggcgctgc ggcatcagcg cgagattggc
      781 tatctaccgt agactcatta gagcttcttg agattctcgt cggcgagacc cacgagcact
      841 gcctcgccgc tgtctcgatc gatgcggaat tccccgtacc tcgcgcgctc gtagcgggcg
      901 gccgtacctt cctcgaagaa gtacgcgttg gtgccgagcc agacctgtcc attgcgcacg
      961 cgatagcgaa tgcgcagatc agcggtggcg gggctggggt tcagcgttgc gatgccacgg
     1021 gcatccacga atatcggcgc actgcccgct gcttccgcgg atatgttgtt ggcgagttcg
     1081 aatcgcagcg ccatgtagtc gccctgcatg agcgagcgcg gatccacggg cgcgagcgcg
     1141 agaaaaatgc ggtcaccctg ggtcttgatg cgctccttgg cgaagatgga ccaattgacc
     1201 gcgccgagca ccagcacgac ggcaatggcc agcagtgcgc ggacttgcgt ggcgttcatg
     1261 ccacctcccc cgattcgggc ttgcggttgc gcaatgcgac gcccgcgagc agcagcgcgg
     1321 cgccggcaca cagcatgatg agcgacttcc acatcagcgt ggtgccatac agatagtaga
     1381 agcgcgacag gtgcaccagc gctgcaaaca cggcgaaacc caacaggcca ccgctgcgca
     1441 cgcggaaggc gccataggcg gcgaacagcg tgagtccgat cgacagcagc gggaatatcg
     1501 ccagccagtc gattcccctg ccccagctat cggcgcccca gatcaccagc gtgaatggct
     1561 cgccgacgat cgctccgatc gacagcccta acaacaggcc ggtcagcagc gggcgggcga
     1621 gccgccgcag gccgctcgcc atccactgcg gctcgcgcag gatgagccac gcggccagcg
     1681 ccagcagcgg cagccacgtg agcagccagg tgacgggaag tgtccacgcg ccgagcgcgg
     1741 cgacatcgcc ggcgtgccgc tcgtcgaaga gaacctgctc ggcgctgccc ggtcgcacca
     1801 ggaagcgcac catccatatc cacgcgatgg tggcgaacag cgccgccagt gtccgcgcaa
     1861 tcttgttagg catgacgagg agtacgaata tctgcaccac cagcgctgtc acgcacatgc
     1921 caagaccggt gtagtaatcc ttgagcacgg acccagccac cgcgatctgc ccggcgatgg
     1981 agaccgcgag cgcgagctga tccaggaaca cgccgtggcg atccgcgtga tacatgaacc
     2041 aggccgccac cagcagcagt gcacccaggc cgaagatcat gggaccggat tcaggcttga
     2101 acacgagccc gatgaaaatc agcaggaaaa tccccgccag ccagcccgcg acgccctgga
     2161 gcagcgacac gaaccacggc cgatccgcgg actccccagg gggtgccggt gcatccctgg
     2221 ggattacgcc ctgcgcgcgc atcagcgcgg aaaattcgtt ggcggcgtcg ctcatgccgc
     2281 agcttccgcg cgccagcggc gattgagcac cgtgagcaca cgcccggcgg ccgtcgatgt
     2341 gctgatgagc cagagtgcca gcatcagcaa catgccttcg tcgtcgaacc cgttcaactg
     2401 tgccagccac acgaggctga tgatgatgaa cgcgcccatg accaccgcaa gtggatagac
//
LOCUS       DASQJS010000398         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2145020, whole genome shotgun sequence.
ACCESSION   DASQJS010000398 DASQJS010000000
VERSION     DASQJS010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2145020"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VEW08_15595"
     CDS             <1..383
                     /locus_tag="VEW08_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HYJ42216.1"
                     /translation="TIETTVALERAIVPLKGFMLEQTRETDIATFVRISGGKGFAKPA
                     DVPLSILVPAFVTSELKTAFQIGFLLFIPFVIIDLVVASVLMSMGMMMLSPVLISLPF
                     KIMLFVLIDGWALVMGTLAQSFFR"
     gene            388..657
                     /gene="fliQ"
                     /locus_tag="VEW08_15600"
     CDS             388..657
                     /gene="fliQ"
                     /locus_tag="VEW08_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HYJ42217.1"
                     /translation="MTPDMVVTIGRHALEMTLMLSAPLLLTALAVGLIVGIFQAATSI
                     NEMTLSFIPKLLAMAAVLALTGPWMIRSLVEYSRNLIESIPSLVG"
     gene            654..1433
                     /gene="fliR"
                     /locus_tag="VEW08_15605"
     CDS             654..1433
                     /gene="fliR"
                     /locus_tag="VEW08_15605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HYJ42218.1"
                     /translation="MTIALSIGQLEGWVAQGFFPFARIGACLMVAPVFGARFVPARTR
                     VILAVATTALVAPMIPTPAIAPFSPQGFVVVFQQLLIGVALGFALQIVFDALGLAGQL
                     LANSMGLSFAFNVDPLRGSSTPALGQLYIILATLTFLALDGHAALIELLVAGLYALPV
                     GTTGLGQEGLWGLILWSGQLFAGAIAVALPGITALLIVNLAFGVVSRAAPSLNLFAVG
                     FPVSLVIGLLVVLSGIGPMQESFTRLLAQGFEFLRALHGGP"
     gene            1437..2537
                     /gene="flhB"
                     /locus_tag="VEW08_15610"
     CDS             1437..2537
                     /gene="flhB"
                     /locus_tag="VEW08_15610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HYJ42219.1"
                     /translation="MAESGAERTESATPKRLEEARKKGQVPRSIELSMAAVCVAAAAA
                     IYTLGDTAAGKFADLMRGSLTLSPQQAVGEGVIWPVLMNAGLRALLIILPILGATFIA
                     ALAAPLAIGGWNISGQALMPQFSKLSPAKGLGRMFSARGSVELGKGIAKVFVVSMIGW
                     VLLSGLTPQMMGLSSEPLHGAIAHSAALAGYALLVLCFGLVAIAAFDVPFQLWQHSRD
                     LKMTREEVKEEYKESEGSPETRGRIREAQRALARGRMMQEVPKADVVITNPTHYAVAL
                     RYDESRNRAPVVVAKGSDLLAARIREIATEHGVPLVAAPPLARALFRNVELGHEVPAA
                     LYVTVAQVLTYVYQLKAAMARGATPPPPPVIH"
     gene            2568..>3033
                     /locus_tag="VEW08_15615"
     CDS             2568..>3033
                     /locus_tag="VEW08_15615"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HYJ42220.1"
                     /translation="MSPLMRLGLGVPIGLISILAMMILPLPPVVLDVLFTFNIALSLV
                     IVMAVFNVKRPLEFAIFPTVLLLVTILRLALNVASTRVVLMHGHEGSHAAGKVIAAFG
                     EFVIGGNFAVGAVVFIVLTIINFVVVTKGAGRVSEVSARFALDAMPGKQMAID"
BASE COUNT          433 a          989 c          992 g          619 t
ORIGIN      
        1 gcacgatcga gaccacggtg gcgctcgagc gcgccatcgt tccgctcaag ggcttcatgc
       61 tcgaacagac gcgtgagacc gacatcgcga cgttcgtgcg catttccggc ggcaagggat
      121 tcgcgaaacc ggccgacgtg ccgctcagca tcctggtgcc ggcgttcgtc accagcgagc
      181 tcaagactgc gttccagatc ggcttcctgc tgttcatccc gttcgtgatc atcgacctcg
      241 tggtcgcgag cgtactcatg tccatgggca tgatgatgtt gtcgccggtg ctgatctcgt
      301 tgccgttcaa gatcatgttg ttcgtgctca tcgatgggtg ggccttggtc atgggcacgc
      361 tcgcccaaag tttcttcagg tgacccgatg actcctgaca tggtggtgac gataggccgg
      421 cacgcgctcg agatgacgct catgctctcg gcgccgctgc tgctcacggc gctggccgtt
      481 ggtctcatcg tcggcatttt ccaggcggcg acgtcgatca atgaaatgac gctgtcgttc
      541 attccgaagc tgctggccat ggccgcggtg ctggcgctca ccggcccgtg gatgatccgc
      601 tcgctggtcg agtattcgcg caacctcatc gaaagcattc cgagcctcgt tggatgacca
      661 tcgcgctctc catcgggcag ctagaaggct gggtggcgca gggtttcttc ccgttcgcgc
      721 gcatcggcgc ctgcctgatg gtggcgccgg tgttcggcgc gcgcttcgtg ccagcgcgca
      781 cccgcgtgat tctcgccgtg gccaccaccg cgctggtcgc gccgatgatt cccacgcccg
      841 ccattgcgcc gttctcgccg cagggcttcg tcgtggtgtt ccagcagctg ctgatcggcg
      901 tggctctcgg tttcgcgctg cagatcgtgt tcgacgcgct cgggctcgcc ggccagctgc
      961 tggccaacag catggggttg tcgttcgcgt tcaacgtgga tccgctgcgc ggcagcagca
     1021 cgccagcgct gggccagctc tacatcattc tcgccacgct cacgtttctc gcgctcgacg
     1081 ggcatgccgc gttgatcgag ctgctcgtcg cgggtctgta cgcgctgcct gtgggcacga
     1141 ccgggctcgg gcaggaaggg ctgtggggtc tcatcctctg gagcggtcaa ttgttcgccg
     1201 gcgcgattgc cgtggcgctg cccggcatca ccgcgctgct catcgtgaat ctcgcgttcg
     1261 gtgtcgtgag ccgcgcggcg ccgtcgctga acctgttcgc ggtcggtttt ccggtgtcgc
     1321 tggtcatcgg cctcctggtc gtgctgtccg gtatcgggcc catgcaggag agtttcacca
     1381 ggttgctcgc gcagggcttc gaattcctgc gcgcgctgca cggagggccg tgagtcatgg
     1441 ctgagtccgg cgcagaacgc acagaaagtg caacaccgaa gcgcctcgag gaggcgcgca
     1501 agaaaggcca ggttccgcgc tccatcgagc tgtcgatggc ggcggtctgc gtcgcggccg
     1561 ccgcggctat ctacacgctg ggcgacaccg ccgcgggcaa gttcgccgac ctcatgcgcg
     1621 gcagcctcac gctgagtcca cagcaggccg tgggcgaggg cgtcatctgg cccgtgctca
     1681 tgaatgccgg cctgcgcgcg ctgctgatca tcctgccgat cctcggcgcg acctttatcg
     1741 ctgctctcgc tgcaccactg gcgatcggcg gctggaatat ttctggccag gcactgatgc
     1801 cgcagttttc caagctgagt cccgccaagg ggctcggccg catgttctcc gcgcgtggca
     1861 gcgtggaact cggcaagggc atcgccaagg ttttcgtggt gtcgatgatc ggctgggtgc
     1921 tgctctccgg gctgacgcca cagatgatgg ggttatcgag cgagccgttg catggcgcga
     1981 ttgcccactc ggcggcgctc gccggttacg ccctgttggt gctgtgcttc ggcctggtgg
     2041 ccattgccgc ctttgacgtg ccgtttcagc tctggcagca ctcgcgcgat ctgaagatga
     2101 cgcgcgagga ggtcaaggaa gaatacaagg agagcgaggg ctcgcccgaa acccgtggcc
     2161 gcatccgcga ggcgcaacgc gcgctggcac gcggccgcat gatgcaggaa gtgccgaagg
     2221 cggacgtggt catcaccaat ccgacgcact acgcggtcgc tctgcgctac gacgagtcgc
     2281 gcaatcgcgc gcctgtcgtt gtagccaagg gctcggacct gctcgcggcg cgcatccgcg
     2341 aaatcgccac cgagcatggc gtgccgctgg tggcggcgcc ccctctggcc cgcgcgctgt
     2401 tccgtaacgt cgagcttggc cacgaagtcc cggccgcgct ctacgtcacc gtggcgcagg
     2461 tgctcaccta cgtctaccag ctcaaggctg ccatggcgcg tggcgccacg ccgccgccgc
     2521 cccccgtcat ccactaaaac gccatggcag aaaacaacag ttccgcgatg agtccgctga
     2581 tgcgcctggg tctcggcgtg cccatcggcc tcatctcgat tctcgcgatg atgatcctgc
     2641 cgttgccccc ggtggtgctc gacgtgttgt tcacgttcaa catcgcgctg tcgctggtca
     2701 tcgtcatggc ggtgttcaac gtcaagcggc cgctcgagtt tgcgatcttc ccgaccgtgc
     2761 tgctgctcgt gaccatcctg cgcctggcgc tcaacgtcgc ctcgacgcgc gtggtgctca
     2821 tgcatggcca cgaaggttcg cacgccgccg gcaaggtcat tgcggcgttc ggcgagttcg
     2881 tcatcggcgg caactttgcg gtgggcgccg tggtgttcat cgtcctcacc atcatcaact
     2941 tcgtggtggt gaccaaaggc gcggggcgcg tgtcggaagt gagcgcacgt ttcgcgttgg
     3001 atgcgatgcc tggcaagcaa atggccatcg acg
//
LOCUS       DASQJS010000399        10104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1828820, whole genome shotgun sequence.
ACCESSION   DASQJS010000399 DASQJS010000000
VERSION     DASQJS010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828820"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VEW08_15620"
     CDS             complement(<1..644)
                     /locus_tag="VEW08_15620"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HYJ42221.1"
                     /translation="MSERSGKHMRLSGTNLERAGDHNQRVTLHAIRVNGPITRAELAA
                     ITGLTSPAIANITKRLLTDTLIQEAGRRRGGRGQPATKLVINPDSWFSIGLNVDRDHI
                     TMVVLDFEGKIRARASTEVNFALPKDVEQFFRKGVGKLLTKVGIGRDRLVGIGVALPD
                     DMPRAALPQQPANYGIWASTDVASLLRETLHVPVFVENDAAAATMGEMQFGLGK"
     gene            complement(641..1585)
                     /locus_tag="VEW08_15625"
     CDS             complement(641..1585)
                     /locus_tag="VEW08_15625"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYJ42222.1"
                     /translation="MSPATKLFAGVELGGTKCVCILGTGPDDVRAVERLPTGEREESL
                     RQIEAVLDRWRAQHGPARALGIASFGPVDLRANSPTYGFITSTTKPGWRNTDIAQRLG
                     RRMGGPVGFDTDVNGAALAEGRWGAAVGLDDFAYVTVGTGIGAGLIVRGKSIFGMNHT
                     ELGHIRVARKPGDKFPGVCPFHGDCIEGLASGPAIEARAGMPASQLPPDHPTWEFVAH
                     GLAQLVHTMVLTTAPSRIFLGGGVMSAQSHLFERIQQELKRSLNRYVEAPELEQGIGQ
                     FVVPPGLGNMAGPLGALALAADAENASIVPPLVTAASR"
     gene            complement(1587..3128)
                     /locus_tag="VEW08_15630"
     CDS             complement(1587..3128)
                     /locus_tag="VEW08_15630"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HYJ42223.1"
                     /translation="MEPDRRIRRVAIVGGGVAGWTAAVMLGKKLGGQCSIHVVESPEP
                     VFPGLAEATQPTILELLRFLGIDQNDFVDKTQSTYSLGTRYIDWAAQGQGFWHPFGGL
                     GALIERRPFYHFWHKARALGLSPRLEACSQEVSLAMANRFIFPTNALGVAAHLRYSLH
                     VDAGITARYLRTIAERAGVIRLERKIVSATRREDGFLDELQFEDGGKLRADLYIDCSG
                     SRAQIIGEMLESPYESWQQWLPCDRLVHTSGPLEEIRPPFVRIGGRAAGWLWRIPLQQ
                     NLSHGLVYSSAHQDDETALQELLAVVGAAPFTAPRRQEFIQGVRRKSWEKNVVALGNA
                     SGFLEPLAATDLHLTTNALFNLLDHFPDKQFDPSNIASYNALIADEFERIRDFLILHY
                     SLSRRDDSPFWQERGKVVLPDALAQRIDIYRATGRIISQRPEVFSDLDWFWIFEGSGV
                     IPRDYDPLVDTVDFEQVKRLMSGFVQKVAADSAAAPSHDSFFAQANSKLASARKAAAA
                     AQTAG"
     gene            3277..5847
                     /locus_tag="VEW08_15635"
     CDS             3277..5847
                     /locus_tag="VEW08_15635"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo 1,3/1,4-beta-D-glucan glucohydrolase"
                     /protein_id="HYJ42224.1"
                     /translation="MSPSMSHVRKHGAALLALAPFLLMLGCKDAAREEPAPAAKVEDP
                     AAVHPEIWPSPKWPFPEDAALEEKVASLLKKMTVEEKVGQVIQGDICCIKPEDMKQYH
                     LGSILAGGGSSPGNNERSPAKDWLKLADEFYAASVDTSNGGVGIPMIWGIDAMHGHSN
                     IVGAVLFPHNVGLGATHNPKLLGDIARVTAEQVRTTGIEWTFAPTVTVPQDDRWGRAY
                     EGYSEDPKLVASYAGEFVKGLQGDPASPDFLKGAHVISSTKHYLADGGTENGRDQGDS
                     KASETELRDIHNAGYVPAIEAGVQTVMISFSSWQGQKLSGHKGLITDVLKKRMNFDGF
                     TVGDWNGHGQVAGCTNDNCAAAFNAGMDMFMAPDSWKGLYENTLKQAKDGTISMERLD
                     EAVTRILRVKFRAGVFDGAAPSKRPLGGDFKMLASPEQRALARDAVRQSLVLLKNNGG
                     LLPLAANKHVLVTGEGADNISKQNGGWTITWQGTGLTNADFPGATSVWGGVKAAVKAG
                     GGSAELSTDGSFKKKPDVAIVVFGENPYAEFQGDLKVQTLPGSMTQQLEIMKKLQDEK
                     IPVVAILFSGRPLWQNRELNLANAYVAAWLPGTEGGGISDVLFRKKDGSVNFDFTGRL
                     SYSWPRDATGTPLNVNKEPYDPLFPFGFGLSYTATADLAALPEDPGIPADLMSTGSYF
                     DKGAPVQPWSLRVSSGTDTTRITTVPTEAVGGRVKVSAVDDAVQEGGRRFQFDGSGPA
                     TIAISSEGAADVSRESNGDVMLLVRLRRDADVPKDVTLGVSCGAGCGGKVPFADTLGG
                     IDSGKWQTVGVPLKCFVKAGADVSKLNETLSMESSGKLDVSLSQVKLGTVADKVVSCN
                     "
     gene            complement(5902..6168)
                     /locus_tag="VEW08_15640"
     CDS             complement(5902..6168)
                     /locus_tag="VEW08_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HYJ42225.1"
                     /translation="MATGWAQDGAVQDQIDATVKDGIKRATSRLPSGPSLESCAECGA
                     KIPEARQQAIPGVRLCVTCQEKEDREVVNNAGYNRRGSKDSQLR"
     gene            complement(6227..6964)
                     /locus_tag="VEW08_15645"
     CDS             complement(6227..6964)
                     /locus_tag="VEW08_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HYJ42226.1"
                     /translation="MSQVSRRSLIAGSLGAGLALPAMAFADEQCGERPNTAPALQPPL
                     LLTDWAWLARYRDDNAKLLASGAKVHAVFLGDSITEGWAARQPEFFSNGIVGRGISAQ
                     TTPQLLVRMHADVVALKPRVVHIMAGTNDIAHNTGPMTAQDSQNNIMAMCEIARAHKL
                     RIVLGAVPPASKYWWRPEVQPKAEALALNEWMRGYARKIGAKYADYAAVLSDANGNVK
                     PELSKDEVHPTPAGYEAMRPVAEAAIK"
     gene            complement(6961..8334)
                     /locus_tag="VEW08_15650"
     CDS             complement(6961..8334)
                     /locus_tag="VEW08_15650"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HYJ42227.1"
                     /translation="MTESTHLGTRNLTIIVTASLAGLLFGFDTVVMSGVTQSLRDVFH
                     LEPGSWWFGFAVASALFGTTLGAMFAGIPGDALGSRDSLKAVGFLYVISAVGSALCWS
                     LPSFYVFRFIGGLAIGASSVLAPVYISEIAPANRRGALTGLFQFNIVFGILLAYVSNF
                     VVQWLAGDFDAWRWKLGVAAVPAFVFLLLMYTIPQSPRWLSLRGREAEAKASLERLGV
                     ADTAGMLAEFARAANEARAAAAHVLFSARYSRPILLAILLAMFNQLSGINAILYYLND
                     IFAAAGFDKMSSDLQAIAIGAANLIATMIALRVIDRFGRKKLLLIGAVGTAVAQAGVA
                     LIMGSGQGNEYLLAMLIMFIAFFAFSQGAVIWVYLAEIFPTAVRARGQALGSTTHWLM
                     NALIATVFPMIAAKAHGVPFAFFAACMVVQFFVVLAVFPETRGVELESMDKAIEKTSE
                     KKSGAAA"
     gene            8550..8939
                     /locus_tag="VEW08_15655"
     CDS             8550..8939
                     /locus_tag="VEW08_15655"
                     /inference="COORDINATES: protein motif:HMM:NF037222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42228.1"
                     /translation="MRKLNVKVRATTFDAERVVLVKLTNRGKAPALGTQLTLVNAEGQ
                     PILPAMYSDNCLTVLPGEPLMVTIRYSAEVVDSAAIRVEGWNTRAASARIPAESTAPT
                     YLQPWQQQIYTPPSPTVKSATVVVPKR"
     gene            8994..9464
                     /locus_tag="VEW08_15660"
     CDS             8994..9464
                     /locus_tag="VEW08_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42229.1"
                     /translation="MRATYLATAVCASVLALPFAVSMLAKVLTGSSNGWNAALSFVLA
                     LFLFVLPAARAFRRLFGTGTVIPPELVHERPVVAWVVTLAGGFGHFFFVISVSAAFEI
                     ALEPERGEDVGGPLAGAFILTLLLYLIALLCAELALVGDGKSDASRGLRSGPFS"
     gene            9511..>10104
                     /locus_tag="VEW08_15665"
     CDS             9511..>10104
                     /locus_tag="VEW08_15665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ42230.1"
                     /translation="MPAPDIARVSAEIDKIYRKDSRRIFATLIRLLGDFERAEEALHE
                     AFRAAMEKWPAEGLPQNPVSWLISAGRFKSIDKQRRDQRFEHLEDRPDISETLTDHSP
                     AFDEREELEDDRLRLVFTCCHPALSPDTQVALTLREVCGLTTEEIAAAYLAPAPTIAQ
                     RIVRAKAKIRDANIPYEVPGRDALPERLDSVLRVVYLV"
BASE COUNT         1879 a         3264 c         3188 g         1773 t
ORIGIN      
        1 ttcttgccga gcccgaactg catctcgccc atggtggccg cggcggcatc gttttccacg
       61 aataccggaa cgtgcaacgt ttcacgcagc agtgaagcga catcggtcga ggcccagatg
      121 ccgtaattcg ccggttgctg aggcagcgcg gcccgcggca tgtcgtcggg cagggccacg
      181 ccgataccga ccagccggtc gcgcccgatg cccaccttcg tgagtagttt gccgacgccc
      241 ttgcggaaga actgctcgac gtccttcggc agcgcgaagt tcacctcggt ggaggcgcgc
      301 gcgcggatct tgccctcgaa atcgagcacc accatggtga tgtgatcgcg gtcgacattg
      361 agcccgatcg aaaaccagct gtccgggttg atgaccagct tggtcgccgg ctggccgcgg
      421 ccaccgcggc gccgaccggc ttcctggatc aaggtgtcgg tcagcaatcg cttggtgatg
      481 ttggcaatgg cgggtgaagt gagaccggtg atggccgcga gctcggctcg cgtaattggc
      541 ccattgacgc gaatggcgtg cagcgtcacg cgctgattgt ggtcgcctgc acgttcgagg
      601 ttggtccccg acagccgcat gtgtttgccg gagcgttcac tcatcgagac gcggcggtca
      661 cgagcggcgg gacgatggaa gcattttcgg catccgccgc gagcgccagg gctccgagtg
      721 gtcctgccat gttgccgagc ccgggaggca cgacgaactg tccgatgccc tgctcgagct
      781 ccggcgcttc gacgtaacga ttgaggctgc gcttgagctc ttgctgaatg cgctcgaaca
      841 ggtgcgactg cgcgctcatc acgccgccac cgaggaagat gcgcgacggc gcggtggtca
      901 gcaccatggt gtggaccagt tgcgcgagcc cgtgcgcgac gaactcccag gtgggatggt
      961 cgggaggcag ttgtgaggcg ggcatgccgg cgcgggcttc gatggcggga cccgaggcca
     1021 ggccttcgat gcaatcaccg tgaaacgggc aaacgccggg aaacttgtcg cccggcttgc
     1081 gcgccacgcg aatgtggccg agctcggtgt ggttcatgcc gaatatcgac ttgccgcgaa
     1141 cgatgaggcc ggcgccgatg ccggtgccca cggtgacgta ggcgaagtcg tcgaggccca
     1201 ccgccgcacc ccaccgccct tcggcgagtg ccgcaccgtt gacgtcggta tcgaaaccca
     1261 ccgggccacc catgcgccgg ccgaggcgct gcgcgatatc cgtgttgcgc cagcccggct
     1321 tggtggtcga ggtgatgaag ccgtaggtcg gcgagttcgc gcgcaggtca acgggcccga
     1381 acgacgcgat acccaacgcg cgtgcgggac catgctgcgc acgccagcga tcgagcacgg
     1441 cctcgatctg gcgcaatgac tcttcacgct caccggtcgg caggcgctcg accgcgcgca
     1501 cgtcatcggg gccggtgccg aggatgcaga cgcacttggt gccacccaat tcgacgccgg
     1561 cgaatagttt ggtggcgggc gacatgtcag ccagccgttt gtgcggccgc ggcggccttg
     1621 cgcgcgctgg cgagcttcga attcgcctgc gcgaagaagc tgtcgtgtga gggtgcggct
     1681 gcggagtccg cggcaacttt ctggacgaag cccgacatca ggcgtttgac ctgctcgaaa
     1741 tcgacagtgt cgaccagggg atcgtagtcg cgcggaatga cacccgaacc ttcgaagatc
     1801 cagaaccagt cgaggtcgga gaacacctcc gggcgctggc tgatgatgcg gccggtggca
     1861 cgatatatat cgatgcgttg ggcgagcgcg tcgggcaaca cgaccttgcc gcgttcctgc
     1921 cagaacggcg agtcgtcgcg ccttgaaagg ctgtagtgca ggatgaggaa gtcacggatg
     1981 cgttcgaact cgtcggcaat gagggcgttg tagctcgcga tattcgaggg gtcaaactgc
     2041 ttgtccggga agtgatcgag cagattgaac aatgcattgg tagtgagatg caggtcggtg
     2101 gcggccaacg gctcgagaaa ccccgaagca ttccccagtg ccaccacgtt cttttcccag
     2161 gatttccggc gcacgccctg gatgaattcc tgtcgacggg gcgccgtgaa tggcgcggcg
     2221 ccgaccaccg cgagcaactc ctgcaaagcc gtttcgtcgt cctggtgagc gctggagtag
     2281 accaggccgt ggctcaggtt ctgctgcaac ggaattcgcc acaaccagcc cgcggcgcgc
     2341 ccaccaatgc gcacgaacgg cggacggatc tcctcgaggg gtcccgaagt gtgcaccagt
     2401 cgatcgcacg gcagccattg ctgccaactt tcgtagggcg actcgagcat ctccccgatg
     2461 atctgcgcgc gtgaaccgct gcagtcgatg tacaggtcgg cgcgcagctt gccgccgtcc
     2521 tcgaactgaa gttcgtcgag aaaaccgtct tcgcggcgcg tggcgctaac aatcttgcgc
     2581 tcgaggcgga tgacaccggc gcgctcggct atcgtgcgca ggtagcgcgc cgtgatcccc
     2641 gcgtccacgt gcagtgaata acgcaaatgg gccgcgacgc ccaaggcgtt ggtcggaaaa
     2701 atgaagcggt tggccatggc cagggacact tcctggctgc aggcctcgag cctgggcgaa
     2761 agccccagcg ctcgcgcctt gtgccagaag tgatagaacg ggcggcgctc gatgagcgcg
     2821 cccaacccgc cgaacggatg ccagaagccc tggccctgcg cagcccaatc gatgtaccgg
     2881 gtgccgaggc tataggtgga ttgcgtcttg tcgacgaagt cgttctggtc aattccgagg
     2941 aatcgcagca actcgaggat ggtcggttgc gtggcttcgg ccaggcccgg aaatacgggc
     3001 tcaggcgatt cgacgacgtg aattgaacat tgtccgccga gtttcttgcc cagcatgacg
     3061 gcggcggtcc aaccagcaac accaccaccg acgatggcaa cccgcctgat tcttcgatcc
     3121 ggttccattt actcccaacc tatccgcccc gacgcggcgg cgaggctatc tgtgcgaccc
     3181 gaaacctgtc aaacaacgcg aaggaagctg tgacgaccct cgaatttccg ccgcagagag
     3241 cataggctgt cggcgctcaa aaacaggagt ttttcgatgt ccccttccat gtctcatgtc
     3301 cgtaaacatg gcgcggcgct gttagcgctc gctccgttcc tgttgatgct tggatgcaaa
     3361 gacgcggcca gggaagaacc cgcccccgcg gccaaagtcg aagatccggc agcggtccat
     3421 cccgaaatct ggccgtcccc gaagtggccc tttcctgaag acgcagcact cgaagaaaag
     3481 gttgcctcgc tgctcaaaaa aatgacggtc gaggagaaag tcggccaggt aatccagggc
     3541 gacatctgct gcatcaagcc cgaggacatg aagcaatacc acctgggctc gatccttgcc
     3601 ggtggcggtt cctcgcctgg caataacgag cgctcacccg cgaaagattg gctcaaactc
     3661 gccgacgagt tctacgccgc gtccgtcgac accagcaatg gtggcgtcgg catcccgatg
     3721 atctggggca tcgacgccat gcacggccac agcaacatcg tgggcgccgt gctgtttccg
     3781 cacaacgtgg gcctcggcgc cacgcacaac cccaagttgt taggcgacat cgcgcgcgtc
     3841 accgccgagc aggtacgcac caccggcatc gaatggacgt tcgcgcccac cgtgaccgtg
     3901 ccgcaggatg accgttgggg ccgtgcctac gaaggctatt cggaagatcc gaaactcgtg
     3961 gcgtcctacg ccggcgagtt cgtgaagggt ctgcaaggtg atcccgcctc gcccgatttc
     4021 ctcaagggcg cgcacgtgat ctcgtcgacc aagcactacc tggccgacgg tggcaccgag
     4081 aacggccgcg accaaggcga ctcgaaggcc tcggagaccg agctacgcga catccacaac
     4141 gccggttatg tgccggccat cgaagccggc gtgcagaccg tcatgatctc cttctcgagc
     4201 tggcagggtc agaagctcag cggccacaag ggcctgatca ccgacgtgct caagaagcgc
     4261 atgaacttcg acggcttcac cgtcggtgac tggaacggtc atggccaggt agccggctgc
     4321 accaacgaca actgcgccgc ggctttcaat gccggcatgg acatgttcat ggcgccggac
     4381 agctggaagg gcctgtacga gaacacgctc aagcaggcga aggacggcac catttcgatg
     4441 gaacgcctcg atgaggccgt gacgcgcatt ctgcgcgtga agttccgcgc cggcgtgttc
     4501 gatggcgccg cgccgtcgaa gcgtccgctg ggtggcgact tcaagatgct ggcctcgccc
     4561 gaacagcgcg ccctcgcgcg cgacgccgtg cgccagtcgc tggtgctgct caagaacaac
     4621 ggcggactgc tgccgctcgc ggccaacaaa cacgtgctgg tcaccggcga aggcgccgac
     4681 aacatcagca aacagaatgg cggctggacc atcacctggc agggcaccgg cctcaccaac
     4741 gccgacttcc ccggcgcgac atcggtgtgg ggcggcgtca aggccgcggt gaaggccggc
     4801 ggaggctcgg ccgagctttc cacggatggc agcttcaaga agaagccgga cgtggccatc
     4861 gtcgtgttcg gcgagaaccc gtacgcggaa ttccagggcg acctcaaggt gcagacgctg
     4921 ccgggttcga tgacccagca actcgaaatc atgaagaagc tgcaggacga gaaaattccc
     4981 gtcgtggcca tcctgttctc gggacgcccg ttgtggcaga accgcgagct gaacctcgcc
     5041 aatgcatacg tcgcggcctg gctgccgggc accgaaggcg gcggcatcag cgatgtcctg
     5101 ttccgcaaga aggatggctc ggtgaacttc gacttcaccg gcaggctttc gtattcctgg
     5161 ccgcgcgatg ccacgggcac gccgctcaac gtgaacaagg agccctacga tccgctgttc
     5221 cccttcggct tcgggttgag ctacacggca accgcggacc tggcggcgct gccggaagat
     5281 ccgggaattc ccgcggatct catgtccacc ggttcgtact tcgacaaggg agcgccggtg
     5341 cagccctggt cgctgcgcgt gtcgagcggc accgatacca cgcgcatcac caccgtcccg
     5401 accgaggccg tgggtggccg cgtgaaggtc agcgccgtgg atgacgccgt gcaggaaggc
     5461 gggcgccgct tccagttcga cggcagcggc cctgcgacca tcgcaatctc gagcgaaggt
     5521 gcggccgacg tgtcacgtga atcgaacggc gacgtcatgt tgctggtgcg cctgcgtcgc
     5581 gacgcggacg tgccgaaaga cgtgacgctc ggcgtgagct gcggcgcagg ttgcggcggc
     5641 aaggtgccgt tcgcggacac gctcggtggt atcgattccg gcaagtggca gaccgtcggt
     5701 gtgccgctca agtgcttcgt caaggccggc gccgacgtca gcaagctcaa cgagacgctg
     5761 agcatggagt cgagcggcaa gctcgatgtc tcgctctcgc aggtcaagct cggaaccgtg
     5821 gccgacaagg tagttagctg caactgaaaa acggggtcag gcaccgtttc ccaggaaacg
     5881 gtgcctgacc ccttttcttt cctagcgaag ctggctgtcc ttgctgccgc gccggttgta
     5941 tccggcgttg ttgacgacct cgcggtcttc cttttcctgg caagtcacgc acaggcgcac
     6001 gcctggaatc gcctgctggc gcgcctcggg aatcttcgcg ccgcactccg cacatgactc
     6061 gagggacggc cccgacggca agcggctggt cgcccgcttg atgccgtctt tgaccgtcgc
     6121 atcgatttga tcctgaactg cgccgtcttg agcccaaccc gttgccatgg gcgtatttat
     6181 ggcccctggc gaatcgattg caagccggac aatccctacc cgacttctac ttgatcgccg
     6241 cctcggccac cgggcgcatg gcctcgtaac ctgctggcgt cgggtgaacc tcgtccttcg
     6301 aaagctccgg cttcacattg ccgttggcgt ccgagagcac ggcggcgtaa tccgcgtatt
     6361 tcgcgccgat tttcctcgca taaccgcgca tccactcgtt cagcgcgagc gcctccgcct
     6421 tgggctgcac ctcgggccgc caccagtatt tcgacgccgg cggcaccgca cccaacacga
     6481 tgcgcagctt gtgcgccctg gcgatctcgc acatcgccat gatgttgttc tgcgagtcct
     6541 gcgccgtcat cggcccggtg ttgtgggcga tgtcattggt gcccgccatg atgtgcacca
     6601 cgcgcggctt cagcgcgacg acgtccgcat gcatgcgcac cagcagctgc ggcgtggtct
     6661 gcgcgctgat gccacgcccc acgatgccgt tgctgaaaaa ctccggctgc cgggccgccc
     6721 agccttcggt gatcgagtcg ccgagaaaca ccgcgtgcac cttcgcgccg ctggccagaa
     6781 gcttcgcgtt gtcgtcgcga taacgcgcga gccacgccca gtcggtgagc agcaacggtg
     6841 gctgcaacgc gggcgcggtg ttgggccgtt cgccgcattg ctcgtcggcg aatgccatcg
     6901 ccggcaatgc cagcccggcc cccagagaac ctgcgatcaa cgatcgacga ctgacctgac
     6961 tcatgctgct gctcctgatt tcttttcact cgttttttca atggccttgt ccatcgactc
     7021 cagctccacg ccgcgagttt ccggaaacac cgcgagcacc acgaagaact gcaccaccat
     7081 gcaggcggcg aagaatgcga acggcacgcc gtgcgctttc gcggcaatca tcggaaacac
     7141 cgtggcgatc agcgcgttca tgagccagtg cgtggtgctg cccagtgcct gtccccgtgc
     7201 gcgcaccgcc gtcgggaaga tctcggcgag atagacccag atcaccgcgc cctgcgaaaa
     7261 cgcgaagaat gcgatgaaca tgatcagcat cgcgagcaga tattcgttac cctgcccgct
     7321 gcccatgatc aacgccacac cggcttgcgc caccgccgtg cctaccgcac ctatcaacag
     7381 cagcttcttg cgcccgaacc tgtcgatgac gcgtaacgcg atcatggtgg cgatgagatt
     7441 cgcggcgccg atggcgattg cctgcagatc cgaggacatc ttgtcgaagc ccgcggccgc
     7501 aaatatgtcg ttgaggtagt aaagaatcgc gttgatgccg gagagctggt tgaacatggc
     7561 cagcaggatc gcgagcagga tcggccggct gtaacgcgcg gaaaacaaca cgtgagccgc
     7621 cgccgcgcgc gcttcgttcg ctgcgcgcgc gaattcggcg agcattcccg cggtgtcggc
     7681 cactcccagg cgctcgaggc tggcttttgc ttcggcctcg cgcccgcgca ggctgagcca
     7741 acgagggctt tgcggaatcg tgtacatcaa caacaggaac acgaatgccg gcaccgcggc
     7801 cacgcccagc ttccagcgcc aggcgtcgaa atcaccggct aaccactgca ccacgaaatt
     7861 gctgacgtac gccagcagga tgccgaacac gatgttgaac tggaacagcc cggtgagtgc
     7921 acctcggcgg ttcgcgggtg cgatttccga gatgtacacc ggcgccagca ccgacgaagc
     7981 gccgatggcg aggccgccga tgaagcggaa cacatagaac gacggaaggc tccagcacaa
     8041 cgcgctacct accgcggata tcacgtaaag gaacccgacc gccttcaagg agtcgcgtga
     8101 accgagcgcg tcgccgggaa tgcccgcgaa catggccccg agcgtggtgc cgaacagcgc
     8161 actcgccacg gcgaagccaa accaccagct gccgggctcg aggtggaaca cgtcgcgcag
     8221 ggactgcgtg acgcccgaca tgaccaccgt gtcgaaaccg aacaacaggc ccgcgagact
     8281 cgcggtgacg atgatcgtga ggttcctggt gcccaggtgg gtagattccg tcatcgcttc
     8341 ccctcgttgc ccgtcgtata tatataagag agcgggctaa ttaactaaaa acggtttagt
     8401 tatagtacgg caacgcctgg ggaggtggaa tcatgtcgat tcgaatctgc gcgtggcctt
     8461 gcgtacctct aacaatcttg attgtgtcgt tctcgaccgc ggtcacggca aattgtgtcg
     8521 ccaccacacc cgaagcgggt gaacgtccca tgcgcaaact caacgtcaag gtgcgggcca
     8581 ccacattcga tgcggagcgc gtggttctcg tgaagctcac gaaccgcggc aaggcgcccg
     8641 cgcttggcac gcagctcacg ctcgtgaacg cagagggaca acccatcctg cccgcaatgt
     8701 acagcgacaa ctgtctcacg gttctgcctg gcgaaccgct catggtcacc attcgatatt
     8761 ctgccgaggt agtcgattcg gcggcgattc gtgtcgaggg ctggaacacc agggccgcgt
     8821 cggcgcgcat ccccgccgaa tcaacagcgc caacctatct acagccgtgg cagcagcaga
     8881 tatacacacc tccctcgccg accgtgaaat ccgccaccgt ggtggtcccg aagcgctagc
     8941 gaggcgcagg atgccgtgac agactggcgt gcatgagtcc cgggaatctc atcgtgcgcg
     9001 ccacctatct cgccacggcg gtctgtgcgt cagtgttggc cctgcccttc gcggtcagca
     9061 tgttggcgaa ggttctcacc ggatcatcga atggctggaa tgccgccttg agctttgtcc
     9121 tcgcgttatt cctcttcgta ctccccgccg cccgcgcgtt ccgccggctg tttggcaccg
     9181 gcaccgtcat tccgcccgag ctcgtgcatg aacgcccggt cgttgcctgg gtagtgacgc
     9241 tggccggagg gttcggccac ttcttcttcg tgatctccgt gtccgccgcc tttgaaattg
     9301 cactggagcc cgaaaggggc gaggacgtcg gcggtccgct cgcgggagcg ttcatcctga
     9361 cgctgctcct ttacttgatc gccctgctct gcgcagagct ggcgctcgtg ggcgacggca
     9421 agagcgacgc gtcgcgcggc ttgaggtctg gcccctttag ctagccaaat tctggagact
     9481 ggggggtctg gccccattta tcctccgggg atgcctgctc cagacatcgc gcgcgtcagc
     9541 gccgagatcg acaagatcta ccgcaaggat tcgcgccgca tcttcgcgac gctcatccgc
     9601 ctgctcggcg acttcgagcg tgccgaagaa gcgcttcatg aagccttccg cgcggccatg
     9661 gagaaatggc cggccgaagg cctgccgcag aatccggtgt cctggctgat ctccgccggg
     9721 cggttcaagt ccatcgacaa acagcgtcgc gaccagcgct tcgagcatct cgaagaccgt
     9781 cccgacatca gcgaaacact gacggaccat tcacctgcct tcgacgagcg cgaagagctg
     9841 gaggatgacc gcctgcgcct ggtgttcacg tgctgccatc cggcgctctc gcctgacacg
     9901 caagtggcgc tcacgctgcg cgaggtttgc ggcctgacta ctgaagagat tgccgcggcg
     9961 tatctcgccc ccgcgccgac gatcgcgcag cgcatcgtgc gcgccaaggc caagatccgc
    10021 gacgccaaca tcccgtacga agtgcctggc cgcgatgcgt tgccagagcg tctcgacagc
    10081 gtgctgcgcg tggtctacct ggtg
//
LOCUS       DASQJS010000400         4560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_888177, whole genome shotgun sequence.
ACCESSION   DASQJS010000400 DASQJS010000000
VERSION     DASQJS010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888177"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3251
                     /locus_tag="VEW08_15670"
     CDS             <1..3251
                     /locus_tag="VEW08_15670"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HYJ42231.1"
                     /translation="PRLRTPGLIAIAGVLCTSGCTNGSKTDPVTEFSTELILTGDAPV
                     VTKRALQRGVYLIEAREQEIDVHMAVIVGGNRSELGDRIPRYGTICQVVSLDGPGELQ
                     VQLRSKDHNTKQGRVALRIARWTRAPGATATERELGFTAFGAAGVQVALATTESSASA
                     ADKMHEAVAHFEAADDEAMLALAAYSLAYVQYGARDQWAAAVRATEIATEAYRAADDE
                     AGIQNAETLRAAAEIQLASAMNAGTQRAEQRALYANADRRLADAAEFFKSHALRIRAQ
                     YAVNMRAVLAVNLGEYTDAASLLAQSAEMARINEDVAEQAKSLGNLAAVHTYLGYIAQ
                     AAQEYEALLPLIDPEVQPYQYAMLLGNYGYTLIALGDFDKALSLRLQALDLYTKAGEE
                     EERAVELAALGGLYFRMGDAERALETLRAAIIEQERISDTTGLASTLRVAANAASVLG
                     QHAVALGYLRKSAQIDANPQTVARTRVLIAKELRDLGDLRSAEAELAAPMESTNALVR
                     AAALEERAHLRLAQRSTVSAIDDLRAADRQYTALGLEFNRIDTNTALSQALLSIRDVK
                     GAAAAASEAISIVSRIRAKSANPEWRARFLSARYAPYEALIAANLAIADEAALWRTFR
                     TAEVVRARSLADELAQGATGAIRKADPEEEELRARLTSQQLRLEARVQRQDADEAGTL
                     ALRHAIEETRALLDANRLRQGGVAARQTSLPESLNLVQQQLPKDIAVLAYFVGDNRSH
                     AWLLTRSELRHAELPGREALQRAIGAFLTARRSGVPRATGERDLGAMLLGNLLDGTAT
                     SRMLVLADGPLNSVPFASLQVPGSGGEMLVDRFVLGYAPSLALALENAQPSKTRSTRV
                     AVVSDPVYAADDRRLRLAMAGETGTLRGAPAASPDNLTRLPYSALEASAVTKAFGAES
                     TIQLSGFDATAERVLQLPSRDLAVLHFATHAVARKDSPDQSALYLSAYASNGAALPAS
                     RLSASDIARSGLRADVVVLSGCATGDGSALRGEGVLGLTYGFLANGSHSVVAALWPIE
                     DASTARFMNEFYQAYRASGRAADALRVAQIRTRGNAATAVWSSFVVRANDFP"
     gene            3305..3853
                     /locus_tag="VEW08_15675"
     CDS             3305..3853
                     /locus_tag="VEW08_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42232.1"
                     /translation="MYGNLRIGGRKLAIWMTAATLTLGAALAGAQEAAPDPALGGWLG
                     KEYTVEYSTLNEHMPTGGKLTFVFDAEDNVVRICTRNSSRQRNPWRMDFAAPCGVTLV
                     FTRGTRYCTLDDVKTGNAEVLSACHRLRSRDIAMRPSKVKGAVELNDMIAFLIQENGK
                     PTMSILVDSPARVTDDGIIQLK"
     gene            4090..>4560
                     /locus_tag="VEW08_15680"
     CDS             4090..>4560
                     /locus_tag="VEW08_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42233.1"
                     /translation="MFSKRHQSAADAAQKARQVPARLIMFPAEGASQRQLVEWLKHTG
                     YVRNLERTRNFVVVVFSRYIPTDRLIEIINSPLVGYSEPDCDGPDYLTTSMELPSAAV
                     STSRCWLRAARSTFPNDPCLDELWGHRKIGWDSGIAARSLPRIVAVLDSGIDAAH"
BASE COUNT          821 a         1467 c         1457 g          815 t
ORIGIN      
        1 ttccgcggtt gcgcactcct ggcctgatcg caattgccgg tgtgctttgc accagcggct
       61 gcaccaatgg gtccaagacg gatccggtca ccgagttttc gactgaactg attctcaccg
      121 gtgatgcgcc ggtcgtgacg aagcgcgcgc tgcaacgcgg cgtatacctc atcgaggccc
      181 gcgaacagga gatcgacgtg cacatggcgg tgatcgtcgg cggcaatcgc agcgagctcg
      241 gggatcgcat tccgcgctac ggcaccatct gccaggtagt tagtctggac ggacccggag
      301 agctgcaggt gcaactgcga agcaaggatc acaataccaa gcagggccgg gtcgcgctgc
      361 gaatcgcgcg ctggacgcgc gcgcccggcg cgaccgccac cgagcgtgag ctcggattca
      421 cggcgtttgg cgcagccgga gtgcaggtcg cgctcgccac caccgaatcc tcggcgagtg
      481 ccgccgacaa gatgcacgag gcagtcgctc actttgaagc ggcggacgat gaggcgatgc
      541 ttgcgctcgc tgcctattca ctggcctacg tgcaatacgg cgcacgcgac cagtgggcgg
      601 cggcagtgcg cgcaacggaa atcgcgaccg aggcgtaccg cgccgcagac gacgaagccg
      661 gaattcaaaa cgcggagact ttgcgcgccg ccgcggaaat tcagctggcc agcgccatga
      721 acgccggtac gcaacgcgcc gaacaacgtg ctctgtacgc gaacgccgat cggcgtctcg
      781 cggatgctgc cgagttcttc aagagccatg cgctgcgcat acgcgcccaa tacgcggtga
      841 acatgcgcgc cgtattggcc gtgaacctcg gtgagtacac ggacgccgca tcgttgctgg
      901 cgcaatccgc ggaaatggcg cgaatcaacg aggacgtcgc cgaacaggcg aagtcgctcg
      961 gcaacctcgc cgcagtgcat acctaccttg gttacatcgc gcaggcagcg caggagtacg
     1021 aggcgctgct gcccctgatc gaccctgaag tccagcctta ccaatacgcc atgctgctcg
     1081 gcaactatgg ctacacgctc attgccctcg gcgacttcga caaggcgttg tccctgcgtc
     1141 ttcaagcgct cgacctttac accaaggctg gagaagaaga agagcgcgca gttgagctgg
     1201 ccgcactggg cgggctctac ttccgtatgg gcgatgccga acgcgcgctc gagacgctgc
     1261 gtgccgcgat catcgaacag gagcggatat ccgacaccac cgggctggcg agcacgctgc
     1321 gtgtcgccgc caacgccgcg tcggttctgg gtcagcacgc ggtcgcgctc ggttatttgc
     1381 gcaagtccgc gcagatagat gccaatccgc aaaccgtggc gcgcacccgc gttctcattg
     1441 cgaaggagct gcgtgacctc ggcgatctgc gttctgcgga agccgaactg gctgcgccga
     1501 tggaatccac caatgcgctg gtacgcgcgg ccgctctcga ggaacgcgcg cacttgcggc
     1561 tcgcacaacg gtcgaccgta tcggcgatag acgacctgcg ggcggcggat cggcaataca
     1621 ccgcgctcgg cctggagttc aatcgaatcg acaccaacac ggcgctgtcc caggcgctgc
     1681 tgagcatacg cgacgtaaag ggcgccgccg ccgccgccag cgaagcgata tcgattgtga
     1741 gccgaattcg cgccaagtcg gcgaatcccg aatggcgcgc gcggtttctg tcggcgcggt
     1801 acgcgccata tgaagcgctc atcgccgcga atctcgccat tgccgacgag gccgcgttgt
     1861 ggcgcacctt ccgtaccgcc gaggtcgtcc gtgcccgctc gctcgccgac gaactcgctc
     1921 agggtgcgac cggcgcgatt cgaaaggctg accctgagga ggaagagctt cgggcgcgac
     1981 tgacctctca gcagttgcgg ctggaggctc gcgtccagcg gcaggacgcc gacgaagcgg
     2041 gcacgctcgc attgcggcac gcaatcgagg aaacgcgtgc gctgctcgat gccaaccgcc
     2101 ttcgccaggg cggcgttgcg gcaagacaga cgtcgcttcc ggagtcgctc aacctcgtcc
     2161 agcagcaatt gccgaaggac atcgcggtgc ttgcgtattt tgtcggtgac aacaggtcgc
     2221 acgcctggtt gctcacacgc agtgagctgc ggcacgcgga gcttccaggc agggaagcgt
     2281 tgcagcgcgc cattggcgca ttcctgaccg cgcggcgtag cggtgtgccc cgtgctaccg
     2341 gtgagcgcga cctcggtgca atgttgttag ggaaccttct ggatggcacg gcgacaagcc
     2401 gcatgctggt gctggccgac gggccgctca acagcgtgcc gttcgcatcg cttcaggtgc
     2461 cgggaagtgg cggcgagatg ctggtcgatc gcttcgtact gggatacgca ccatcgctgg
     2521 cgctcgcgct tgaaaatgcg cagccttcga agacgcgcag cacccgtgtc gcggtcgtgt
     2581 ccgatcccgt ctacgccgcg gacgatcgac gcctgcgcct ggccatggcc ggcgaaaccg
     2641 gcaccttgcg cggcgcgccg gcggcctccc ctgacaacct gacgcgcttg ccctactcgg
     2701 cgctggaggc gagcgcggta acgaaggcat ttggagccga gagcacgatc cagttgtcgg
     2761 ggttcgatgc caccgcggag cgcgtgctgc aacttccttc ccgcgatctc gccgtactgc
     2821 actttgccac ccatgcggtg gcgcgcaagg attccccgga ccagtctgcc ctctacctga
     2881 gcgcatacgc gtcgaatggc gcggcattgc ccgccagtcg cctgagtgcc agcgacatcg
     2941 cgcgcagcgg gctgcgcgca gacgtggtgg tgttgtcggg ctgcgccacc ggggatggca
     3001 gcgcgctacg tggcgagggc gtactcggac tcacatacgg atttcttgcc aatggttcgc
     3061 actcggtcgt tgcggcgctg tggccgatcg aagacgcgtc cacggcacgc ttcatgaacg
     3121 agttctatca agcctatcgc gcgtccgggc gtgccgccga cgcattgcgc gttgcgcaga
     3181 ttcgcactcg tggcaatgcc gcgaccgcgg tgtggtcgag tttcgtagtt cgggctaacg
     3241 attttccgtg acgcccgcgg gcaataggtc tttccaaacg gctgcattga atgaggagtg
     3301 gctcatgtac gggaatttga gaattggtgg acggaaactc gcgatctgga tgacggcggc
     3361 aacgctgacg ttgggggcag cactcgccgg ggcccaggaa gccgcaccag atccggcact
     3421 tggtggatgg ctcggcaagg aatacacagt cgaatactcg acgctcaacg aacatatgcc
     3481 gacgggtggc aaactgacat tcgtgttcga cgccgaagac aacgtcgttc gcatctgcac
     3541 gcgcaattcc agcaggcagc gaaacccctg gcgcatggac ttcgcggcgc cttgcggtgt
     3601 cacgctcgtt ttcactcgcg gcacgcggta ttgcacgctc gatgacgtga agacgggaaa
     3661 tgccgaagtc ctttccgcct gccatcgcct gcgatcgcgt gatatcgcga tgcggccatc
     3721 gaaggtgaag ggcgcggtcg aactcaacga catgattgcc ttcctgatcc aggaaaacgg
     3781 caagccaacc atgtcgatcc tggtcgactc gccggcacgt gtgaccgacg acggcatcat
     3841 ccagctcaag tagtaatcgg agaaaatttg cgctcgcttg cgcgccgact ctcaacaatg
     3901 ttgctgccgc ttgccgcggc aacgacgttt ggggcgtcgt gcgcgcttgc ggccgcaccg
     3961 aatctctgca ctcacggcgg tagatgggcg tcgacggtcg cccggtctcc tccccccgtc
     4021 acggcttatc tcgattctct cggcaatgcc cgcttcgatc tccgggccgc ctgcgctcac
     4081 tgggcgggca tgttctccaa gcgtcatcaa tccgcggcag acgccgcgca gaaggcgcgg
     4141 caggtacccg cccggctcat catgtttccc gccgaaggcg ccagtcagcg tcagctggtc
     4201 gaatggctca agcacaccgg gtatgtccgg aatctggaac gcactcgcaa ttttgtcgtc
     4261 gtcgtttttt cgcgatatat cccgaccgac cggctcattg aaatcatcaa ttcgccgctg
     4321 gtgggctatt cggagcccga ctgcgacggg ccagactacc tcaccacttc gatggagctg
     4381 ccgtccgcgg cggtatccac atcgcgttgc tggttacgcg ccgcgcgatc cacttttccc
     4441 aacgatcctt gtcttgatga gttgtggggt cacaggaaga tcggctggga ttcaggcatc
     4501 gccgcccggt ccttgcctcg catcgtcgcg gtgctcgatt cgggcatcga cgccgctcac
//
LOCUS       DASQJS010000401        15191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3087945, whole genome shotgun sequence.
ACCESSION   DASQJS010000401 DASQJS010000000
VERSION     DASQJS010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15191
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3087945"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..302
                     /locus_tag="VEW08_15685"
     CDS             165..302
                     /locus_tag="VEW08_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42234.1"
                     /translation="MPLLLRLLPLMPLPLRLLTLPLLRPLTLLLRLLMPPPLRLKRRS
                     N"
     gene            400..930
                     /locus_tag="VEW08_15690"
     CDS             400..930
                     /locus_tag="VEW08_15690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ42235.1"
                     /translation="MTVSVRDARGAPRDREWIESVYSDYLDDLSPLNTGVFSATSLGE
                     IGYSESDQLARWFGDSSVNALVILRGAEPVGFAMVARGSASPGKSPVDYRMAEFFVAR
                     TFRRRGVGRLAVELILNRFAGRWEITEYLRNPVAVAFWRRVVANYTQGRFQERVLNGE
                     VRQVFESGNSRAQKPA"
     gene            938..2647
                     /gene="ggt"
                     /locus_tag="VEW08_15695"
     CDS             938..2647
                     /gene="ggt"
                     /locus_tag="VEW08_15695"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYJ42236.1"
                     /translation="MRRASFIALLLIMTMEGGYAGSRPSGSLVATRSEVIATHGMAAT
                     SQPLVSQVALDVLKRGGTAVDAAIAANATLGLMEPTGNGVGGDLFAIVWDAKTQKLYG
                     LNGSGRSPKSLTLQKLRAELKKLNSATIPKTGPLPVSVPGTVDGWFELHSKFGKLPMK
                     ELLAPAIAYARDGFPVTEVIAEGWARNARNLQKYPGFAETYMPNGRAPAKGEVFRNPL
                     LANTLAAIAEGGRDAFYMGDIAKRIESYMRANGGYLTAADLADHHSEWVEPVSTNYRG
                     YDVWELPPNTQGVAALQMLNILEAYDLKSMGFGSAEYLHLFIEAKKLAFEDRARYYVD
                     PEFAKIPLKGLLSKEYAAKRRALIKLDRAALEYPTDAKALEQGDTIYMTVADSAGNMI
                     SLIQSNYRGMGSGMTPTGCGFILQDRGELFSLEDGHANVYAPGKRPFHTIIPAFITKD
                     GKPWLSFGLMGGAMQPQGHVQIVVNLIDFGMNLQEAGDVPRARHDGSSEPTGEVMKDG
                     GEVVLEVGALPATVKALQARGHKVTIANDGDFGGYQAIMRNAEGVYFGASESRKDGAA
                     QGY"
     gene            complement(2795..4744)
                     /locus_tag="VEW08_15700"
     CDS             complement(2795..4744)
                     /locus_tag="VEW08_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42237.1"
                     /translation="MRFDKTLICLLAWALLFDAAHAAPQKASFASDVSTTQWPLADLD
                     PQLPLDWSQAEFLVVEFRSSTSQRFELGLVSDEGNVSKRIHPFPKVWVRASIPLRFFR
                     QGLGDADELASTVNQPRNSYWINIEAGGHAPVLHVRALSVTMRYPARTSTVEIRKVSL
                     SKTDPGDAVLDGGGPLIDDLGQYVHADWSGKARAYPELIREWAAENRMLVPKATIQTC
                     RYGGYAGGRRKPTGYFRVEKISDRWWFIDPEGCRFYSTGVNGAGAEPPRTQIIGRSKL
                     FASIPTAAQFPMNAEPDPLRDPVSFYAANLQKRFGSDWRGPSAQLTSRRMRAWGLNTA
                     YGAALNEALPEGSSLRQPYVFPLRGWQHIEGAIMGLPDVYSEAFARRVDAEAAQQLGP
                     RKTDPWMIGYFIGNEPPWPARESQLVDLVLAGPASSIQQRFKIELVKGDSPAIRKALV
                     HAAFTRYLQIVNAAVKRADPNHLNLGIRFGGTPPDEVIALARGFDVYSMNKYRWAPPK
                     DFIDRVYAIQKLPILIGEFHFGVPERGLAPGLVQAMNQTERGVAYSYYVEHAAEHPAI
                     IGTHWYQWIDQPATGRRDGENYNIGWIDVTDRPYPELVAAAKATHAKLDDIHSGRLAA
                     TTRRPKASEFGTPEDSIYLGIPAIQ"
     gene            complement(5735..6019)
                     /locus_tag="VEW08_15705"
     CDS             complement(5735..6019)
                     /locus_tag="VEW08_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42238.1"
                     /translation="MTRMVAVIDWLPVDVQVAPVMPPPPLLEDELVEDELVELELVLL
                     LDVELLLELDDPVPPYEQYRGLPTLIDGNREAEQVIGPVRVAYTKLPDFP"
     gene            6009..6569
                     /locus_tag="VEW08_15710"
     CDS             6009..6569
                     /locus_tag="VEW08_15710"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HYJ42239.1"
                     /translation="MRVTSGTYDGGCKTFNPSGALGDGSQEEGQDPAFRVENGATLRN
                     AILGNNGVDGVHFYGGGNLQNFRWTNVGEDAYTVKSSGTVNISGVSGFNGEDKFGQIN
                     AASTVNISNCIVDNMAKFVRQNGGTTFKITINADRCQISNMKEGVFRSDSSSSVARLT
                     NSRLRNSGSLCIGSWSSCTGSGNTNY"
     gene            6706..7491
                     /locus_tag="VEW08_15715"
     CDS             6706..7491
                     /locus_tag="VEW08_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42240.1"
                     /translation="MAAILVGAAVYWIFSPGPNVARELRPTAAPPPGSKDEVRSRHTA
                     QLDTAFALPSTSAPSVDPESSTIARYTSEKYQFLLDDLRYLEASQVEQLQRALQLRER
                     LSGELNSAGQDGALARAEDQIRGLLSPADHATYEMLRESDLELFNLNEYAGGISNVAP
                     LSAADRESILKTKLAYKARFRLLLRDSGLQRSDQSPAEREYAFSMTARALEDFRRSYL
                     QEVQQYLANEEQFALLSSYEATEFKAELAQLQSMIDTPEQGGS"
     gene            7578..8534
                     /locus_tag="VEW08_15720"
     CDS             7578..8534
                     /locus_tag="VEW08_15720"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HYJ42241.1"
                     /translation="MFSRILCGTALLAFAGMAQATISLSASPGNAQVSLSWSYSNETP
                     VTQEVYRDTDSNPSGRVRIGSVSSSTRSFTATGLSNGTTYYFWIKNTVNGVATNSNTA
                     SATPAGSGGGGGAITGATCSSSGSTSITATIRVTSGTYDGGCRTFNPSGALGDGSQEE
                     GQDPAFRVENGATLRNAILGSNGVDGVHFYNGGNLQNFRWTNVGEDAFTIKSAGTVNV
                     SGVTGVNSEDKFAQVNAESTLNVSNCIVDNAGKFLRQNGGTTFKITVNVDRCQISNMG
                     EGIFRTDSSSSVARLTNSRLRNSGSLCIGPWASCTGSGNTNY"
     gene            8627..9436
                     /locus_tag="VEW08_15725"
     CDS             8627..9436
                     /locus_tag="VEW08_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42242.1"
                     /translation="MSLRGVVIPALVVFVAIVWWSSRHAGEPASDAASANPAPSSATQ
                     SEWPQLATGNHESNDLAPSAQFAVEVGADASFGAYVDGKYRFLEPSEKLRAALLEREK
                     IAVAINTARQSNDTAAKDAIPGLLAELAALDQKIGTLLRPGDLATYEVLKDSDIEQFQ
                     LDDYAGGVNAVAPVSDTDRRAILYTKLVHRQRFRQVLADSRLMTGELNASERQFAFAE
                     VSRALAESRDGYLQEVRQYLYNDEQFALLRNYENSEYAAELEKLRGIAGDE"
     gene            complement(9444..10580)
                     /locus_tag="VEW08_15730"
     CDS             complement(9444..10580)
                     /locus_tag="VEW08_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42243.1"
                     /translation="MPSAIFTRRFAANYFSTLGFVGLSYWVISDLSGFHRGMLQGQWQ
                     FGMFEIDAVLSIRTLLLTLIALYAVVLIPYYARYPWLHSKAFVFLRGLQFSFLRVRRP
                     QSPARRALQARQPWRLPLNAGLSPPAKQAGLALLLKFFFAPLMINWCLNHAGDMANSV
                     VQFFDGVHEGVSGRQLFDSSLFWACFQLILFIDTLLFTIGYIVEVPALNNRIRTVDPT
                     FFGWAICLACYPPFNDFTSRFLEWQSSDFPQFENTAVHFAANISLLAALSIYSWASVA
                     LGFKASNLTNRGIISHGPYAFVRHPAYAAKNLAWWIGALPNLYLVFISGNVRNAAYAL
                     LALCGWTTIYALRAITEERHLLLTDNGYAEYMKRVKWRFIPGVI"
     gene            complement(10582..12792)
                     /locus_tag="VEW08_15735"
     CDS             complement(10582..12792)
                     /locus_tag="VEW08_15735"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HYJ42244.1"
                     /translation="MAHDTARMRAAQNFLSAIVLSTIVLSTLAGTSTSTAAARDPAPA
                     YVPQVINPELNGGLLVNGSRVLLVWGSDGTILRSEDGVHWVHAITPVSADLARVASNE
                     RGDVLIAVGASGAILRSTDAGRTWLAVQHTTTDTDLRAVVNQPGSRTWVSVGTNGRVL
                     RSIDDGRKWSPIDSQLTVAFQTLFVDPATKAMLIGGDEGMVGYSKNAGESWQITALTM
                     PDPATPVTAFHRFGKLLLATSALGRFLTSGDDGQSWDLMQSSSKAFFTDCALDPVRNA
                     IVMTGHNGDVLRSADGGRSWEVGEIVLDGRKNFLNAIRFDTRSGSLLAIGQGGALARS
                     TDGGASWTRASDGVHGDVRGLINDTTRNRLIAFGTGGMVLSSTDSGARWTEDRGALDL
                     SLREIEPAPRGKALIATSKLGDIIRSADGGASWQPLPITYPNPNTPPDLRGLIVAPSG
                     EALVAVGPPGAILRSNADGSVWDLRHSTPIEAERAFPWVLVDQRRRILVAVEARGAMQ
                     VSNDDGVSWQVSSIPIPQERFPIWQGAVLERAGVMLVAGEAGVAARSTDGARQWSLID
                     TGTRQDLFGSFANEATDTLFLMGAKGTLLRSADLGVSWRGVTSGSTQELRRILRDPRT
                     GALVCFGTHGTILRSSDDGLTWRVVPSVTDGVLRHGMLEPRTGNLLLVGSQGSLLRSR
                     DGGHAWEALPSHTVRHFYSMSVDDRSGDIVLAGERIVRLVRQSGGTTGRANQQP"
     gene            complement(12782..13051)
                     /locus_tag="VEW08_15740"
     CDS             complement(12782..13051)
                     /locus_tag="VEW08_15740"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYJ42245.1"
                     /translation="MEEELKGLEAVLAKMAHDLRTPLAVVHTTTNMLLNPKYKFTEDQ
                     VREQHQRIQRNVEVMDRLITKLSELARPATESATGDSRPTRIDGA"
     gene            complement(13154..14617)
                     /locus_tag="VEW08_15745"
     CDS             complement(13154..14617)
                     /locus_tag="VEW08_15745"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYJ42246.1"
                     /translation="MKSSKTSTTLGFAALAIFTAPLTVAADPGWYFGGNGGPSQTDVD
                     EDRIAADLLSQGFATTFMDDNERDTGFKVFGGYQFNKYFSLESGYYDLGDFGFTSVTV
                     PAGTLHGVLGARGLNLDAVLSVPFTDKFSAFVRAGAAYSEVEGAFEGTGLVNVVEPNS
                     ESKEVNYKFGAGLEYMFTRRFGMRLEAERYRVDDSVGNMGDIDLYSAGVLFRFGAAPA
                     PAYVAPPPPPPPVAVAPPPVVVEPPPPTPVQTDRYCSLLNFEFEINADKVQLAEQEKL
                     AVIGTFLKKYPQTSAVIEGHTDDVGTNEANQRLSERRANAVVSYLVRNLKVDSARLTP
                     VGYGETRPIADNSTDEGKRVNRRIGAIVACATDIEGLYVRPARTTMALAIDFDARDTA
                     IKPEFRNELRGLADFLKANPTVTATVEGHTGNLQATPEQAMVISQQRAQNVVNYLVDN
                     FGIDRSRLTAEGFGQKRRFSYNPQDNRRVNVIVEYPR"
     gene            complement(14655..>15191)
                     /locus_tag="VEW08_15750"
     CDS             complement(14655..>15191)
                     /locus_tag="VEW08_15750"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050825 - ice binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HYJ42247.1"
                     /translation="NSTTGPTSAGPNAVKCAVATQARLDAQAAYLQLAGLAPGANPGA
                     NLGSLTLAPGVYTSPSGSFLIQGGDLTLDAQGDANAIFVFQMAQTLSVGGPGVAAPQS
                     IILAGGAQAKNVFWQVGSFATINAAGGGTMVGTILSQAGASFSTVGNVNLVTLNGRVI
                     SLGASVTLVNTVINVPAP"
BASE COUNT         2884 a         4625 c         4874 g         2808 t
ORIGIN      
        1 ttaccactag atatttttgg ggtaccccta catgaaattg aatgttgctc tcgccgcctc
       61 gatcgcggct ttcgcgctga tgactggctg cgccaagaag gatgcggaag ctcctgctgc
      121 cgacgctgct gcctcgtcgg ccgatgccgc cgcttcttcg gccgatgccg ctgcttcttc
      181 ggctgctgcc gctgatgccg ctgccgctcc ggctgctgac gctgccgctg ctccggccgc
      241 tgacgctgct gctccggctg ctgatgccgc cgccgctccg gctgaagcgc cgaagtaact
      301 aatcgacctc gattagttct tcgcgagcca acaggctcat gacggcccgg gcagagcgca
      361 caacgttctg cccgggcttt gtcttttctg gaggatgacg tgacagtcag tgtccgagat
      421 gcgcgtggcg ccccccgaga ccgcgagtgg atcgaaagcg tctacagcga ctatctcgac
      481 gacctgtcac cgctgaacac cggtgtgttc agcgccacgt cgctgggcga aatcggctac
      541 agcgaatccg accagctggc gcgctggttc ggcgactcct ccgtcaatgc cctggtcatc
      601 ctgcgtggtg ctgagcccgt gggcttcgcc atggtggcgc gtggcagcgc atcgcccggc
      661 aagtcgccgg tcgactaccg catggccgag ttcttcgtcg cgcgtacttt ccgccgccgc
      721 ggcgtgggcc gcctcgccgt ggaactcatt ctcaatcgat ttgccgggcg ctgggaaatc
      781 accgaatacc tgcgcaatcc ggtagccgtc gcgttctggc ggcgcgtggt cgctaactac
      841 acccagggac gatttcagga gcgtgttctc aatggcgaag tgcgtcaggt ctttgaatcg
      901 ggcaactcgc gtgcccagaa acccgcctga ttcattcttg cgccgcgcgt ccttcattgc
      961 gctactgctc atcatgacga tggaaggtgg gtatgctggc tcgcgaccca gcggttcgct
     1021 ggttgccacg cgctccgagg tcatcgccac tcatggcatg gcagccacca gccagccgct
     1081 cgtgagccag gttgcgctcg atgtactcaa gcgcggaggc acggcggtcg acgccgccat
     1141 cgccgcgaac gcaacgcttg gcctcatgga gcccacgggc aacggcgtgg gcggcgacct
     1201 gttcgccatc gtatgggacg cgaagacgca gaagctctac ggtctcaatg gcagcggccg
     1261 ctctccgaaa tcgctgacac tccagaagct gcgtgctgaa ctcaagaagc tgaactccgc
     1321 cacgataccg aagaccggac ccttgccagt gtcggtgccg ggcacggtcg atggctggtt
     1381 cgagctgcat tcgaaattcg gcaagctgcc gatgaaagaa ctgctggcgc cggcgatcgc
     1441 ctacgcgcgc gatggtttcc cggtgaccga agtgattgcc gaaggctggg cgcgtaatgc
     1501 acgcaatctg cagaaatacc ccggctttgc cgaaacctac atgccgaacg gccgcgcgcc
     1561 ggcgaagggc gaggtgtttc gcaatcctct cctcgccaac acgcttgccg caattgcgga
     1621 aggtggccgg gatgccttct acatgggcga catcgccaag cgcatcgaat cctacatgcg
     1681 cgccaatggc ggatacctga ccgcggccga cctggcggac caccattcgg aatgggtgga
     1741 gccggtgtcc actaactacc gtggctacga tgtgtgggag ctgccgccga atacgcaggg
     1801 tgtggctgcg ctgcagatgc tcaacatcct cgaggcctac gacctcaagt ccatgggatt
     1861 cggcagcgcc gagtacctgc acctgttcat cgaggcaaag aagctcgcgt tcgaagaccg
     1921 cgcgcgttac tacgtggatc cggagttcgc gaagattccg ctgaaggggt tgctgtcgaa
     1981 ggaatacgcg gcgaagcggc gcgccctcat caagctggat cgcgcggcgc tcgaataccc
     2041 caccgatgcg aaagcgctcg agcagggcga caccatctac atgacggtcg cggattcggc
     2101 gggcaacatg atctcgctga tccagagcaa ctaccgcggc atgggttcgg gcatgacgcc
     2161 cacgggctgt ggattcatcc tgcaggatcg aggcgagttg ttctcgctcg aggacgggca
     2221 cgccaacgtc tatgcgccgg gcaagcggcc gttccacacc atcattcccg cgttcattac
     2281 caaggacggc aagccctggc tttcgttcgg gctcatgggc ggggccatgc agccgcaggg
     2341 ccatgtgcag atcgtcgtca atctcatcga tttcggcatg aacctgcagg aagccggtga
     2401 cgttccgcgc gcgcgtcacg acggctcgag tgagcccacc ggcgaggtca tgaaggatgg
     2461 cggcgaggtg gtgctcgaag tgggtgcatt gccggccacc gtaaaggcgc ttcaggctcg
     2521 tggccacaag gtgaccattg ccaatgacgg agatttcggc ggctaccagg caatcatgag
     2581 aaatgccgag ggcgtgtatt tcggcgcatc ggagtctcga aaggacggcg ccgcacaggg
     2641 ttattagcgg cgctggcaat tgcggaacct ttgactgtct tgcggctact caccaacgtt
     2701 ggcgccggtg gcgaggtccg cgcgagccct gtttaccgat tgcgggagct cggcatgagt
     2761 ctaggggggt tccgacccat cggcgccagc actgctactg gatcgccggt attcccaggt
     2821 agatcgagtc ctccggcgtt ccgaactctg atgccttcgg tctgcgcgtg gtagccgcga
     2881 gccttcccga gtggatgtca tcgagcttgg cgtgagttgc cttcgccgcc gctacgagtt
     2941 cagggtaggg gcggtccgtg acatcgatcc agccaatgtt gtaattctcg ccatcgcgtc
     3001 gtccggtagc gggctggtct atccactggt accagtgtgt gccgatgatg gcgggatgct
     3061 cggcggcgtg ctccacatag taggagtacg ccacaccacg ctcggtctgg ttcatggcct
     3121 gcacgagtcc cggtgcgaga ccgcgttcgg gcacgccgaa atgaaactca ccgatgagga
     3181 tgggcaactt ctggatggcg tacacgcggt cgatgaaatc cttcggcggc gcccagcggt
     3241 acttgttcat gctgtacacg tcgaatccgc gcgccagcgc gatgacttcg tcgggcggcg
     3301 tgccgccgaa acggatgccg agattcaggt ggttagggtc ggcgcgtttg accgcggcgt
     3361 tcacgatctg gaggtagcgc gtaaacgccg cgtgaaccag tgccttgcga attgcggggc
     3421 tgtcgccctt cacgagttca atcttgaaac gctgctggat tgaactcgcg gggcccgcca
     3481 gcacgagatc gacgagctgg ctttcccgcg ccggccatgg gggctcattg ccgatgaagt
     3541 aaccgatcat ccatgggtcg gtcttgcgcg gccccagttg ctgcgcggcc tcggcgtcca
     3601 cgcgtcgggc gaacgcttcg gaatacacgt cgggcagccc catgatcgcg ccttcgatgt
     3661 gctgccagcc ccgtagcgga aagacgtacg gctgacgcaa tgacgagccc tcgggaagcg
     3721 cctcgttgag cgcggcgccg taggcggtgt tcagccccca ggcgcgcatg cgccgcgacg
     3781 tcagttgcgc actcgggcct cgccaatcgc tgccgaagcg tttttgcagg ttagcggcgt
     3841 agaacgacac cggatcgcgc aacggatccg gctcggcgtt cattggaaac tgcgcagcgg
     3901 tgggaatgct ggcgaacaac ttcgagcggc cgatgatctg tgttcgcggc ggctcagcgc
     3961 ccgcgccatt cacgcccgtg gagtagaagc ggcaaccctc gggatcgatg aaccaccatc
     4021 gatcgctgat cttctccacg cgaaagtacc cggtgggctt acgacggccc ccagcgtagc
     4081 caccgtagcg gcacgtctgg atagtggcct tcgggaccag cattcgattt tctgccgccc
     4141 attcgcgaat gagctccgga tacgcgcgtg ccttgcccga ccagtcggcg tgcacgtact
     4201 gcccgaggtc gtcgatgagc ggaccaccac cgtctagtac tgcgtcgccc gggtcggttt
     4261 tcgagaggct caccttgcga atctcgacgg tcgatgtgcg cgccgggtaa cgcatggtga
     4321 cgctgagcgc gcgcacatgg agcacgggtg cgtgcccgcc ggcttcgatg tttatccagt
     4381 aactgttgcg cggctggttg acggtcgatg ccagttcgtc ggcatcaccc aggccctggc
     4441 gaaagaagcg cagggggatc gacgcgcgca cccagacttt gggaaacgga tggatgcgct
     4501 tggacacgtt gccttcgtcg ctgacgaggc ccagttcgaa gcgctggctg gtcgaggagc
     4561 ggaattcgac gaccaggaat tcggcctggg accagtcgag cggaagctgc ggatcgagat
     4621 cggcgagcgg ccactgcgtc gtcgagacgt cgctcgcgaa ggaagctttt tgtggagcgg
     4681 cgtgtgcggc atcgaacaga agcgcccacg ccagcaggca gatcagcgtc ttgtcgaatc
     4741 tcatcccacc cccggatacg gccgtagttt atgcgaagca gatcgccgcc ttgaaagagg
     4801 gatcctacgt gcggtgcgac ataattccag cggtctggac catccgtcgc agcaagaatt
     4861 gcggcaccga acgaggccag ggccatgcct tcaccgtttt catgtccgcg cgagcccgaa
     4921 tctcggaaaa aacttttctt cacttccgag gcgcgagccg agtcacctca ggctgaagag
     4981 gaatggattg gccgcactga gacaatgcaa tcccaattca ttacttgcat ttcagcactg
     5041 aagaagggcg cgaactgcac gaatgaattc atgttgcacg agtgagtcac gcgcgtttcc
     5101 ggtttgcgac cgcgcgctga aacagcggga aaaatagact cttcgcggag tgtggaagac
     5161 cgagtccacg atggactccg ccgatttgag acaccggtgt catatttccg ccaagaagcg
     5221 gtcaattttt cgcgatgtag cgcgagtttt ccgccgaaag agtcttgtct accggtagac
     5281 ataaaatcgc atggttcggg acattcgacc cccatggtga gacatgtctt cggacaacga
     5341 aattgtttga caagagtttt tttacgggcg taccgtccgg cgccgtcgat gccgacctct
     5401 tcacgggtcg aatgatgaac aagcgaatcg gtatcggcgc atcggtgcac gtgatcgatg
     5461 cagcagcagg gggatgagtt gcgagtcgcc tcattgcggt gcgcaatttg tcatatcgcg
     5521 cgaggctcgg gtgctgacag gcccgggtcg catggaaggt gagaacaact cagaccaatg
     5581 gaggcgtgat gactacgttt agaaatctct tgatcggttc ggcattgctg gcggcatcgg
     5641 cagcgtcaaa tgcagcaacc gactatccgt ccggttacac caagtgcgcc aaggaagggg
     5701 aaacctgttc gttcagcggc acacgttcgg tggcctacgg aaagtccggc agtttcgtgt
     5761 acgcgactct gaccggccct attacctgtt cggcttctct gttcccgtcg atcagtgtcg
     5821 gcaacccgcg gtactgctcg tatggcggca ccggatcgtc gagctccagc agcagctcca
     5881 cgtcgagcag cagcaccagc tcgagctcca ccagctcgtc ttccaccagt tcgtcttcca
     5941 gcagcggcgg cggcggcatc acgggtgcca cctgcacgtc cacgggcagc cagtcgatca
     6001 cggcgaccat ccgggtcacc agcggcacct atgacggcgg ctgcaagacc ttcaatccga
     6061 gcggcgctct gggtgacggc agccaggagg agggccagga tcctgccttc cgcgtggaaa
     6121 acggtgcgac gctccgtaat gccatcctcg gcaacaacgg tgttgacgga gtgcatttct
     6181 acggtggcgg caacctgcag aacttccgtt ggaccaacgt cggtgaagac gcgtacacgg
     6241 tgaagtcctc gggcaccgtc aacatttcag gcgtgtccgg cttcaatggc gaagacaagt
     6301 tcggccagat caatgccgct tcgaccgtga acatctcgaa ttgcatcgtc gacaacatgg
     6361 cgaagttcgt tcgtcagaac ggcggcacca ccttcaagat cacgatcaac gcagaccgct
     6421 gccagatctc gaacatgaag gaaggcgtct tccgttccga cagctcgagc agcgttgctc
     6481 gcctgaccaa cagccgcctg cgcaacagtg gatcgctgtg catcggctca tggtcgagct
     6541 gcacgggttc ggggaacacg aactactgat caggacaagc tgctcgtttt taggatgaaa
     6601 acttgaggct tggccggggg tggaaacacc cccggcatct tccgtttgga gggtctggtg
     6661 agctggaacc cggcacgaag gcgtctacga atcggtatgt ccggagtggc agccatcctc
     6721 gtgggtgcag cggtgtactg gatattcagt cccggcccga acgttgcgcg agaattgcgc
     6781 cccaccgcgg caccaccgcc aggatcgaaa gatgaagtgc gctcacggca cacggcgcag
     6841 ttggataccg ccttcgctct cccgagcacg tcggcgccgt ccgtggatcc tgaatcgtcg
     6901 acgatcgctc gatacacttc tgaaaagtac caattcctgc tcgatgacct tcgttacctg
     6961 gaagccagcc aggttgagca gctgcaacgc gcgctgcagt tgcgcgagcg gctctccgga
     7021 gaattgaatt cggcgggcca ggatggagcg ctcgcgaggg cagaagacca gatccgcggg
     7081 cttctctctc cggccgacca tgcaacctac gagatgctca gggaatccga tctcgagctg
     7141 ttcaacctca acgagtacgc gggcggcatc agcaacgtgg cgccactcag cgcggccgat
     7201 cgcgaatcca tactgaagac gaagcttgcc tacaaggcac gctttcgcct gttgctgcgc
     7261 gactcgggcc ttcagcgcag cgaccagagc ccggccgagc gcgagtacgc gttttcgatg
     7321 acggcacgcg cgctggagga tttcaggcgc agctatctgc aggaagtcca gcagtacctc
     7381 gccaacgagg aacaattcgc gctgctgagc agctatgagg ccacggaatt caaggccgaa
     7441 cttgcccagc tgcaatcgat gatcgacacc ccggaacagg gaggtagtta gccatacatc
     7501 tcgatgacga tgttgatgct ttgaaaacgt gtgcatttca tggaagaaac caatcgttgt
     7561 tacaacaagg cggatatatg ttttcaagaa ttctttgtgg aacggccctg ctcgctttcg
     7621 ccgggatggc gcaggcgacc atctcgctct cggcgagtcc cggcaacgcc caggtgtccc
     7681 tgagctggtc ctattcgaac gaaacgccgg tcacccagga ggtctatcgg gatactgact
     7741 cgaacccgag tggccgcgtg cgtatcggaa gcgtcagcag cagcacgcgc agcttcactg
     7801 cgaccgggct gagcaacggc actacctact atttctggat caagaacacg gtcaatggag
     7861 tggccacgaa ttcgaatact gcgtcggcca cgcccgcggg aagcggtgga ggcggcggcg
     7921 ccatcaccgg cgcgacctgc tcatcctccg gcagcacctc catcaccgcg accattcgcg
     7981 tgacgagtgg cacgtacgac ggcggttgcc gtacgttcaa cccgagcggc gcgctgggcg
     8041 acggcagcca ggaagaaggc caggatccgg ccttccgcgt cgagaacggc gcaacgttgc
     8101 gcaacgccat cctcggcagc aacggtgtcg acggcgtgca cttctacaac ggcggcaacc
     8161 tgcagaattt ccgctggacc aatgtcggcg aagacgcgtt cacgatcaag tccgcgggca
     8221 cggtgaacgt gtccggagtg acgggtgtca acagcgaaga caagttcgcg caggtcaatg
     8281 cggagtcgac gctcaacgtg tcgaattgca tcgtcgacaa cgcgggcaaa ttcctgcgcc
     8341 agaacggcgg caccacgttc aagatcacgg tcaacgtcga tcgctgccag atctcgaaca
     8401 tgggtgaagg catcttccgc acggatagtt cgagcagtgt tgcgaggctg accaacagcc
     8461 gcctgcgcaa cagcgggtcg ctgtgcatcg ggccctgggc gagttgcacg ggctcgggga
     8521 atacgaacta ctgatcaggg ggggagagac gggccggggg tggcagcacc cccggcattt
     8581 ccgtccggag gatgaggtga tggcggaaac gaggacattg cattcagtga gtctgcgcgg
     8641 cgtagtcatt ccggcattgg tggtattcgt ggcgatcgtc tggtggtcga gccgacatgc
     8701 cggcgagccg gcgagcgacg ctgcgtccgc gaatccggcg ccatcatccg cgacgcaatc
     8761 ggagtggccg caattggcca ccgggaatca cgagagcaat gacctggcgc cgtccgcgca
     8821 gttcgctgtc gaagttggcg cggacgccag cttcggcgcg tacgttgacg gcaaatatcg
     8881 attcctcgag ccctcggaaa aacttcgggc cgccttgctc gagcgcgaga aaatcgcggt
     8941 ggcgatcaac accgcgcgac agtccaacga cacggcggcg aaggacgcca tcccgggcct
     9001 gctcgccgag ctggcggcgc tcgaccagaa aattggcacg ctcctgcggc cgggcgatct
     9061 cgccacatac gaagtactca aggattccga catcgagcag tttcagctcg atgactacgc
     9121 tggaggggtg aacgctgtcg cgccggtgag cgacaccgat cgccgggcaa ttctctacac
     9181 gaaactggtg caccggcagc gtttccgcca ggttctggcc gattcgcgcc tgatgacagg
     9241 tgagttgaac gccagcgagc gccagttcgc gttcgcggaa gtgagtcgag cactcgctga
     9301 atcgcgcgac ggttacctgc aggaggttcg tcagtatctc tacaacgacg agcagttcgc
     9361 gctgctacgc aactacgaga acagcgagta tgcggccgag ctcgagaaac tgcgcgggat
     9421 tgccggggac gaatgagcgc gtgtcagatt acgcccggga tgaagcgcca cttcacgcgc
     9481 ttcatgtatt ccgcgtaacc attgtcggtg agcagcaggt gccgttcttc ggtgatggcg
     9541 cgcagcgcgt agatggtggt ccatccacac agcgcgagca gcgcgtaggc ggcgttgcgg
     9601 acattgccgc tgatgaacac caggtacagg ttgggcagcg cgccgatcca ccacgcgagg
     9661 ttcttggccg cgtaggcggg atgccgcacg aaagcatacg ggccgtggct gatgatgccc
     9721 cggttggtga ggttgctggc cttgaacccc agcgccaccg acgcccagga gtagatagac
     9781 aaggccgcca gcagcgagat gttggcggcg aaatgcacgg cagtattttc gaattgcggg
     9841 aaatcactgc tctgccactc gagaaagcgg ctggtgaagt cattgaacgg cggataacac
     9901 gcaaggcaga tggcccagcc gaagaacgtg gggtccaccg tgcggatacg gttgttgagc
     9961 gccggcactt ccacgatgta gccgatcgtg aagagcagcg tgtcgatgaa caggatgagc
    10021 tgaaagcagg cccagaacag cgaggagtcg aacagttgcc ggccgctgac gccttcgtgg
    10081 acgccatcga agaattgcac caccgagttc gccatgtcgc cggcatggtt gagacaccag
    10141 ttgatcatca gcggcgcgaa gaagaacttg agaagcaatg ccaggcccgc ctgcttggcg
    10201 ggcggcgaga ggccggcatt cagcggcagg cgccagggct gtcgagcctg gagagcgcga
    10261 cgcgcgggcg attgcggcct gcgcacgcgc aagaagctga attgaagccc acgcagaaac
    10321 acgaacgcct tggagtgcaa ccacgggtag cgcgcgtagt aaggaatgag caccaccgcg
    10381 tacagcgcga tcagcgtcag cagcagcgtg cggatggaca agactgcgtc gatctcgaac
    10441 atgccgaact gccactggcc ctgcagcatg ccgcggtgaa agccgctcag gtcggaaatg
    10501 acccagtacg aaaggcctac gaagccgaga gtcgagaagt agttggcggc gaagcgccgt
    10561 gtgaatattg cggagggcat gtcatggttg ttggttagcg cgcccggtcg tgccgccgga
    10621 ttgtcgcacg agccgcacga ttcgttcacc ggcaagaacg atatcgccgc tgcgatcgtc
    10681 gaccgacatg ctgtagaagt gacgcaccgt gtggctcggg agcgcttccc acgcgtggcc
    10741 gccatcgcgt gagcgcagaa gcgagccctg actacccacg agcagcagat tgccggtacg
    10801 tggctccagc attccgtggc gcaacacacc gtcggtgaca ctcggtacca cgcgccaggt
    10861 caaaccatca tccgacgaac gcaggatcgt gccgtgtgtg ccgaaacaca ccagcgcgcc
    10921 ggtgcgcggg tcgcgcagta tccggcgcag ttcctgggtg ctgcccgacg tcacgccacg
    10981 ccacgaaacg ccgaggtcgg ctgaacgcag gagggtccct ttcgccccca tcagaaaaag
    11041 agtgtcggtc gcttcattgg cgaagcttcc gaacagatcc tgccgggtgc ccgtgtcgat
    11101 gaggctccac tggcgcgctc catcggtgct gcgcgcggct actccggctt cgccggcgac
    11161 gagcattacg ccggcgcgct ccagcaccgc gccttgccag atcggaaatc gctcctgcgg
    11221 gatcggaatg ctgctgacct gccagctcac gccgtcgtcg ttcgacacct gcatcgcgcc
    11281 acgcgcttcg acggcgacga gaatcctgcg gcgttgatcg accaggaccc acggaaatgc
    11341 gcgttcggct tcgatgggcg tcgagtggcg cagatcccag acgctgccgt cggcgttcga
    11401 gcgaagaatg gcgcccggcg gccccaccgc gaccagtgcc tcgcctgacg gtgcgacgat
    11461 gagcccgcga agatcaggcg gagtattcgg attcgggtag gtgatgggta gcggctgcca
    11521 gctcgcgcca ccatccgcag accggatgat gtcgccgagc ttgctggtgg cgatcagcgc
    11581 tttgccgcgc ggtgccggtt cgatttcgcg cagcgaaaga tcgagcgcgc cgcggtcttc
    11641 ggtccaccgc gctccggaat ccgtcgagct cagcaccatg ccgcccgtac cgaacgcgat
    11701 gaggcgattt cgcgtcgtat cgttgatcag gccgcgcaca tcaccgtgca cgccgtccga
    11761 ggcgcgggtc catgacgcgc cgccgtcggt ggagcgggcc agtgcgccac cctggccaat
    11821 ggcgagcagc gagccgctgc gcgtgtcgaa gcgaatcgcg ttcagaaaat tcttgcggcc
    11881 gtcgagcacg atttcgccaa cttcccagct tcgtccgccg tccgcggagc gcagcacatc
    11941 gccgttgtgc ccggtcatca cgattgcgtt gcgcaccgga tccagggcgc aatcggtgaa
    12001 gaacgccttg gagctcgact gcatgaggtc ccagctctgg ccgtcgtccc cggatgtcag
    12061 aaatcggccg agcgcgctgg tggcgagcag tagtttgccg aagcgatgaa acgcggtgac
    12121 cggtgtggcc ggatccggca tcgtgagcgc ggtgatctgc caggactccc ctgcgttctt
    12181 cgagtagccg accatgccct cgtcgccgcc gatcagcatg gccttcgttg ccggatcgac
    12241 gaacaaggtc tggaatgcga ctgtgagctg cgaatcgatc gggctccact tcctgccgtc
    12301 gtcgatcgag cgtaatacgc gcccgttcgt ccccaccgag acccacgtgc gactgccggg
    12361 ctgattcacg acagcgcgca ggtcggtgtc cgttgtcgtg tgttgcaccg ccagccaggt
    12421 gcgtccggcg tccgtcgatc gcaggatggc cccggaagct cccaccgcga tgagcacgtc
    12481 gccgcgttca ttcgaggcaa cgcgtgcaag gtctgcggac accggcgtga tggcatgcac
    12541 ccaatgcacg ccgtcctcgg aacgaaggat cgtgccatcg ctgccccaga cgagcagcac
    12601 acgcgaaccg ttcacgagca agccgccatt cagctcggga ttgatgacct gtggcacata
    12661 ggccggcgcg gggtcgcgcg cggccgcggt gcttgtcgat gtgccggcca gagtcgacaa
    12721 gacgatagtc gacaagacga tagccgacag gaagttctgc gcggcccgca tccgcgcggt
    12781 gtcatgcgcc atcgatgcgg gtgggtcggg agtcgcctgt ggctgactcg gtagcgggtc
    12841 gcgccagctc cgatagtttc gtgatcaggc ggtccatcac ttccacgttg cgctgaatgc
    12901 gctgatgctg ctcccggacc tggtcctcgg tgaatttgta cttcgggttg agcagcatgt
    12961 tcgtcgtggt atgcacgacg gccaggggag tgcgcaggtc gtgcgccatt ttcgccagca
    13021 cggcttccag acctttcagt tcttcttcca ttcctgaacg gtaacgccga cgaaggagct
    13081 tatccagccc tattgtcagg agcgttgagc ggggcgagcc acgccgcgat cggcgtggct
    13141 cccgatcatc tgatcatctc ggatattcga cgatcacgtt cacccgacgg ttgtcctgtg
    13201 ggttgtaaga gaatcgtcgc ttctggccga agccttcagc agtcagccgc gaacggtcga
    13261 tgccgaagtt gtccaccagg tagttcacga cgttctgggc acgctgctgg gagatcacca
    13321 tggcctgctc gggtgtcgcc tgcagattgc cggtatgccc ttccacggtg gcggttaccg
    13381 tcgggttggc cttgaggaag tcggccaggc cgcgaagctc attgcgaaat tccggcttga
    13441 tggcggtgtc ccgcgcatcg aaatcgatcg ccagcgccat ggtcgtgcgc gcgggacgaa
    13501 catacagtcc ctcgatgtcc gtggcgcagg cgacgatcgc cccaatgcgg cggttcacac
    13561 gcttgccctc atcggtggag ttgtcggcga tgggccgtgt ttctccgtat ccgaccggcg
    13621 tcaaccgtgc actgtcaacc ttcaggttgc gaaccagata actcaccacc gcattggcgc
    13681 ggcgctcgga caggcgctga ttggcctcat tcgtgcccac atcgtcggtg tgaccttcga
    13741 tcacggcgct cgtctgtgga tactttttca ggaacgtgcc gatgacggcg agtttttcct
    13801 gctccgcgag ctggaccttg tccgcgttga tctcgaactc gaagttgagc agtgagcaat
    13861 accggtcggt ctgaacaggt gttggcggcg gcggttccac gacgactgga ggtggggcca
    13921 ctgccacggg cggcggtggc ggcggcggtg cgacgtacgc gggcgcgggt gctgcgccga
    13981 agcggaacaa cacgccggcc gaataaagat cgatgtcgcc catgttgccg acggagtcgt
    14041 cgacccggta gcgctcggcc tccaggcgca tgccgaaccg gcgcgtgaac atgtattcga
    14101 gtcccgcacc gaacttgtag ttcacctctt tgctctcgct attgggctcg accacgttca
    14161 ccaggccggt gccttcgaag gcgccttcca cttcggagta cgccgcgccc gcgcgcacga
    14221 atgcagagaa cttgtcggtg aagggaaccg acagcactgc atcgagattc aggccgcggg
    14281 ctcccagcac tccgtgcagg gttccggcgg gtacggtgac ggaggtgaaa ccgaagtcac
    14341 ccagatcgta gtaaccactc tccagggaaa agtacttgtt gaactgatag ccaccgaaca
    14401 ccttgaagcc ggtgtcgcgt tcattgtcgt ccatgaacgt ggtggcaaat ccctggctga
    14461 gcagatcggc ggcgatgcgg tcttcgtcca cgtcggtctg cgagggaccg ccgtttcctc
    14521 cgaagtacca gccgggatcg gcggcaacgg tcagtggggc tgtgaagatt gcgagggccg
    14581 cgaatccaag cgtcgtcgag gtcttagaag atttcatgtc ggtgtccgtt tgattctggt
    14641 gagtgcgaga cggcttacgg cgccggcaca ttgatgacgg tgttgaccag cgtgacggac
    14701 gcgccgagtg agatcacccg gccgttcagc gtgacgaggt tgacgttgcc gacggtcgag
    14761 aacgacgcgc cagcctggga gaggatcgtg ccgaccatgg tgccgccgcc ggcggcattg
    14821 atcgtcgcga agctgccaac ctgccagaac acgtttttcg cctgcgcacc acccgcgagg
    14881 atgatgcttt gcggagccgc cactcccggg ccgcccacgg agagtgtctg tgccatctgg
    14941 aatacgaaga tcgcattcgc atcgccttgc gcatcgagcg tgaggtctcc gccctggatc
    15001 aggaacgaac cactcggtga ggtgtagacg cccggcgcca gcgtgagcga accgagattg
    15061 gcgcccggat tcgcgcccgg tgcgaggccg gccagttgca gatacgccgc ttgcgcatcg
    15121 agccgcgcct gcgtggcgac ggcgcacttg accgcgttcg gacctgcgga ggtcgggccc
    15181 gtcgtggagt t
//
LOCUS       DASQJS010000402        23567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4341486, whole genome shotgun sequence.
ACCESSION   DASQJS010000402 DASQJS010000000
VERSION     DASQJS010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 23567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23567
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4341486"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..707
                     /locus_tag="VEW08_15755"
     CDS             51..707
                     /locus_tag="VEW08_15755"
                     /inference="COORDINATES: protein motif:HMM:NF023682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HYJ42248.1"
                     /translation="MFDEMPAVADERQTTSAPREYTRAPPEYRVIPCGRGDARRAEAE
                     AFIRKRFLRSHGAHIATFMPTLMLLTGVDSELVAAAGFRSAAQEPLFLERYLPLAIEQ
                     AIASQSGAPVQRAEIVEVGNFAALDSRRAKILMSFMPAYFLEGSARWLVFTATSSIRR
                     ILSAMGGRCLEVGTADGACVAGGIDEWGRYYSTDPRVMAGFLPLARRIPELWRTHHGN
                     "
     gene            697..2172
                     /locus_tag="VEW08_15760"
     CDS             697..2172
                     /locus_tag="VEW08_15760"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYJ42249.1"
                     /translation="MEIDPLLAETPRLTPGRIALTDGIRSITYGQLGSIVRTEADLLR
                     EAGGLRFGLLAENGCDWAVADLAVHHLNRVNVPLPAYFTAAQMRHAIDDAGVDTLITD
                     RRDEVLDRWPEFSVAGTLSSNGLSVLRRAVEPEQRRAVPFGTTKITYTSGSTADPKGV
                     CLPGAALDRASRAMMQITSPIGIRRHLCLMPLPTLLENLAGIHAPLRAGATCVVPSSI
                     DTGMSYGALDPQRLLRSLTLHRPESLVLVPELLRLIVRAAQGGYALPDSLKFIAVGGA
                     SVSRSLLDEAAALGLPVFEGYGLSECGSVVCLNTPQAKRLGSVGRALPHVSLSLGRGG
                     ELRVSGWVMSGYLGDSQTEGPHEIATGDLAEIDADGYVYIRGRLRNIFITSFGRNMSP
                     EWIERELAHEPAIRHALAIGEGCPDVRALVCPAKPELEQTAIEHAVEHANERLPDYAR
                     VRHVVRMPEAPTISNGLLTANGRLRRERILERFGALLEQAA"
     gene            2189..2875
                     /locus_tag="VEW08_15765"
     CDS             2189..2875
                     /locus_tag="VEW08_15765"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HYJ42250.1"
                     /translation="MSNFTELAARTASARTQLQTTPVIARALRGEVTRELYLDFLTQA
                     YHHVRHTVPLLMAVGARLPARQLWLQKELVHYLEEELGHDEWILSDIATAGGDADAVR
                     NGAPSVETDAMVAYAYDTVMRRNPVAFFGMVYVLEGTSVALALNAADAVQRTLALPNK
                     AFTYLRSHGSLDQQHIGDLESIVDRFDASTDLPAVVTCARAMFRLYGNVFRSLESGET
                     AGAMLLQGAA"
     gene            2872..3696
                     /locus_tag="VEW08_15770"
     CDS             2872..3696
                     /locus_tag="VEW08_15770"
                     /inference="COORDINATES: protein motif:HMM:NF006565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ42251.1"
                     /translation="MKLDVMRVLLTGATGGIGSVIAKRLLEEGAHVLLVARDEAALAA
                     LVQSCSFASDRVQAHSVDITQSKSRTALCEVARQWHGGVNVLINNAGLNTFSMYEELT
                     EDQIDQTLAVNLQAPMHLCHDLLPWLRTRESAAIVNVGSVFGSIGLPGYVAYSATKFA
                     IRGFSEALRRELADSRVMVKHLAPRATRTAMNTAAVVEMNSRLKVAMDPPERVARELI
                     ALFKRRRSSAVVGWPEKAFVKLNAVLPGIVDRAVRGQLSIVRAFARRLRPQEIFSK"
     gene            3693..4370
                     /locus_tag="VEW08_15775"
     CDS             3693..4370
                     /locus_tag="VEW08_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42252.1"
                     /translation="MNIKTTLHNWLETLAIAVAFLPLAFPALAEDAPFTAELTGIQQS
                     WDQANYRSANAEDKQRQLEALSARSEALVHKYPQRAEPLVWEGIVLSTYAGAKGGLGA
                     LSLAKKSRDCLLEATRIDAAALRGSAYTSLGALYFKVPGWPIGFGDRDKATTFLNKAL
                     ALNPDGMDPNYFIGELLYEQGEYSRSLQHLQKALAAPPRPDRPVADAGRRAEINTLIA
                     KVSSKLQ"
     gene            complement(4430..5659)
                     /locus_tag="VEW08_15780"
     CDS             complement(4430..5659)
                     /locus_tag="VEW08_15780"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HYJ42253.1"
                     /translation="MKNLIALVLSCALVSTTALAAPDASKPNVRAITAFVRLDRSTYE
                     KQVEEAMKVLNAAKADFAKRGYETQTVRIVTQPFAELVKGLPDKEALAFLRSFDELSQ
                     KAGFAANVGPAMLHDTDDPAAMHLLARVLSTMPNLNASAIIADDDGIHWKTIRESAKL
                     VRYLTDNSPRSQGNFQFTATAMLKPLGPFYPGAYHTGAGKLFSLGFESANVVQEVFGV
                     THGDFDASVAELARQLTIHARVGEQVGEAVAAATGWSFAGVDPTPAPLGDTSIGDAIE
                     RYTGSKFGANGTMTAVLAITSAVKAVKVKQVGYSGLMLPVMEDKRLAQRWAENTYAID
                     SVLAYSAVCGTGLDTVPLPGDVSEERLAKIFGDVAALAWKWNKPLSARLQPVKGKKAG
                     DKTEFDSQYLFNTTLQP"
     gene            5822..6025
                     /locus_tag="VEW08_15785"
     CDS             5822..6025
                     /locus_tag="VEW08_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42254.1"
                     /translation="MKRPNPKMHMPQHDYGQALQTAVSWLGERYLLAEPVSRRIEEPK
                     PFFVVQQSWHEPRRTVGPRSRRH"
     gene            6153..7394
                     /locus_tag="VEW08_15790"
     CDS             6153..7394
                     /locus_tag="VEW08_15790"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HYJ42255.1"
                     /translation="MTIRNFAPRPMKLSVLTAAFQELTPRDRRDPDPDLAIEEWLEFA
                     RDIGSPNIQLSSALHPTLSDVPPEAMLDPVANTLDLRAPFDKSRAARVLKAMKATGVG
                     LSDLGYFDNMLTDDVTARKHKHDFMLRVFDAAALLGANAVCGFVGRNIRLEMDENLVM
                     FEQQFIPLLKEAKARGLTYRVEQCPMPGWNTLDRWHNNIAYAPGPWIALHRICEKHGV
                     GDQFRIHYDPSHAILMGQDTRSLFQYLKDEGYGFLIGGFHVKGQVVDSRGVSAWGYGG
                     QTMQRGDWKDGKPSTNPADAANAWKKQVVLAEHELPGTARHDPLAYLQNRTVDWLDHQ
                     LAARELLSIDPADTYLVVEHEYPPARIQDKARLAPILKGSLAFVKAIDEAAAAMFALQ
                     HEVMAAQGIPIQGKGREAYRS"
     gene            7391..8635
                     /locus_tag="VEW08_15795"
     CDS             7391..8635
                     /locus_tag="VEW08_15795"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HYJ42256.1"
                     /translation="MIGARLSAMMFLQFFIWGCWFVTLGTFLAANFQASGAQTGLAFS
                     TQSWGAIIAPFIIGLIADRYFNAERILAVLHLVGAVLMYQMFKAQDFAQFYPYVLAYM
                     IAYMPTLALVNSISFRQLTDPSRQFSLVRVWGTVGWIVAGLVISFVFHWDSQEAVASG
                     ALRNTFLMGAVMSLLLAIFSLTLPATPPTVAKGGFSLRGALGLDALKLLRDRNFAVFF
                     VSSVLICIPLAFYYQNASPFLTEIGTANATGKLTIGQMSEVAFMLLLPVFLLRFGIKK
                     TLLLGMLAWAVRYVLFAYGGQDNISVLLIIGIALHGICYDFFFVSGQIYTDSKAGEHY
                     RSAAQGMITLATYGVGMLVGFSFAGKIADQFNVDGQHDWFQIWLYPAGIAAVILVLFA
                     LLYRNESITYQKPDLDSRRPSR"
     gene            8648..9445
                     /locus_tag="VEW08_15800"
     CDS             8648..9445
                     /locus_tag="VEW08_15800"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HYJ42257.1"
                     /translation="MPRAKPRSRKPSPPRGYSAPALEKGMDIIELLADSENGLSVSEI
                     SLRLKRRMSELFRIVVVMERRGWLQKDPETSRYCVTYHVLKLAHRGTPTQTLTSAAAP
                     VMQDLSTRINQSCHLVVRSGTQGLVILRQENQLRHANLSVRLGAVIDLVTSCSGQILL
                     AHMEPAERWRLLQSIPCPRGTSKAKVDVELEKVRKRGFEIHRSPITAGVTDLSYPIRD
                     FDDKVVAALTVPYLHALDNSLPTSVEQTRHLLEEATRRISKSLGATR"
     gene            complement(9461..10642)
                     /locus_tag="VEW08_15805"
     CDS             complement(9461..10642)
                     /locus_tag="VEW08_15805"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HYJ42258.1"
                     /translation="MRMLVCALALVAGTCVAEEASRFQPAETNVWGAEYPRIDATGRV
                     QIRIKAAEASKVKLNFWSGPKVDMEKQPDGFWTITTTPLVPGLHYYVINVDGVDASDP
                     GSRAFFGGSRYVSAVEIPEPGSTYYSIQAVPQGQVRDVWYESKVTGSWRHAMVYLPPD
                     YEAQTKKRYPVLYLQHGGGEDETGWIRQGRANVILDNLIAAGEAKPMIIVMAYGYARR
                     AGAPLLNPTPGSPQAAQAREEMAKTFEDDVTQALIPFVDKSFRTLSDRDNRGMAGLSM
                     GGFQTFHITTRHLDLFSHIGGFSGIGGMLEDRKWDFKTDFNGMFADPAAFAKKVHLLY
                     FGVGTAEPERFSTRIRGLHAALTEAGIDHVYWESPGTDHEWQTWRRNLKDFASRAFKK
                     K"
     gene            11014..12585
                     /locus_tag="VEW08_15810"
     CDS             11014..12585
                     /locus_tag="VEW08_15810"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HYJ42259.1"
                     /translation="MSNTLRALAGALACFFLAGTGVAAAADEPVEISVDAANPGAAIS
                     RHLFGQFAEHLGRGVYEGIWVGPDSPIPNTRGIRNDVVAALKAIKVPNVRWPGGCFAD
                     EYHWRNGIGPRDRRPATLNPNWGGVIEPNTFGSHEFLDFAQQIGADAYLSINVGSGTA
                     AEAADWLEYLTTPQPSALGKERAANGHPEPYRIAMLGIGNESWGCGGSMTPDYYVSQL
                     KVHARYARNYNAAQKMQRIAVGPDGAHTEYTKAVMEAWKNKTWAWDIEGISLHFYTVV
                     KWPPAFKATGFGEGEYATLLNDTRRMDDLLRTHLSIMDQYDPAKKIALIVDEWGAWLA
                     PTPGSPEGFLEQQNSQRDALIAALNLNMFARHADRVRGANIAQMVNVLQAMILTNKDK
                     MLLTPTYHVFRLYLPFQDATLLPVKVSAGDYQHGDIKLPRVDAIAARDAQGKTWLAVS
                     NLDPGRATQVSLDVTGAKIKRARGETLTSPRVDSVNTFDAPETVAPKPFEARASSGRL
                     KLSLAPASITVVALE"
     gene            12617..13354
                     /locus_tag="VEW08_15815"
     CDS             12617..13354
                     /locus_tag="VEW08_15815"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HYJ42260.1"
                     /translation="MRVHGTIAREIGLSIVSGRLRPGHVLDGEIEASSQLKVSRTAYR
                     EAIRILSAKGLIRSRPRTGTRVNAISDWHLLDPDVLTWVFTQVPKPEVIHGLFELRTI
                     VEPAAAALAATRRTRIHLAEMRRALDAMALHTLDKVEGREADKAFHAALLAATANPFV
                     ISLTNGVTAAVNALTEFKQRLAKIERDPVPDHLHVFDAIAAKDAEAAREAMIKLIRLA
                     VLDMNVKQRPRPPSGGKLSEAAYFIMV"
     gene            complement(13341..14207)
                     /locus_tag="VEW08_15820"
     CDS             complement(13341..14207)
                     /locus_tag="VEW08_15820"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HYJ42261.1"
                     /translation="MREGLARESSIEVNSLESADIRAGRDFLWPRQKIYVSHLPGQTQ
                     AQTVNTCAQVAAAGFEPVPHVPVRLLKNEQQLDGILSAIRDAGASSLLLISGDYVRPE
                     GPYREVLDVMRTGKLQKFGFTCVSVAGHPEGHPAVAADEIRRAQIEKARCGVAAGLQV
                     TLVTQFFFEATPFVDWARDLRRAGVEARIVAGLPGPATAGRLLALARHCGVGPSIRVL
                     SSRYRSMFKLLSERSPGVLLDDLAAERRRQPDLFDGIHLYSFGGFLRTAAWLRQQAEL
                     VEAQVVPPSTPL"
     gene            complement(14204..15610)
                     /locus_tag="VEW08_15825"
     CDS             complement(14204..15610)
                     /locus_tag="VEW08_15825"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HYJ42262.1"
                     /translation="MNHPNLESLIRSAGDTVRMLRNSPIGAYVYPVVPTEFSNWRDEQ
                     LAWRSGAVLFDQSHHMAEITIQGPDALRLCSHTTINSFASFAPGKAKQMIPVNHDGFV
                     IGDGILFYLEKDELLFVGRAPTVNWLQFHAETAGFDVKVIRDDRSPSQPNGRAVKRRH
                     FRYQVQGPRATQVLQKLYGGPLPDIRFFNFEWLNIAGRRVRALRHGMAGEPGLEIFGP
                     YEERDEMRAAILDAGKEFGIVAVGSRTYASNTLESGWIPSPLPAVYTGEKMRAYREWL
                     PANGYEGTGSLGGSFVSANIEDYYLTPYELGYGSFVKFDHEFIGREALEKRVQESDPH
                     RRKVTFEWNGEDLGRILASLNDRENLPYKFFDLPIANYASSSYDRVKLGDRLVGFSMF
                     TGYSHNERCGLSLGVVDADIEAGTELTLVWGEEDGGTAKPTVERHRQCEVRVKVSPVP
                     YSRDARDSYHAGWRTSPR"
     gene            15702..16619
                     /locus_tag="VEW08_15830"
     CDS             15702..16619
                     /locus_tag="VEW08_15830"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYJ42263.1"
                     /translation="MGELDLNLLRVLVALDDRRSVSAAAAQLQRSQPSVSSALGKLRA
                     FFADPLFVRSGNSMQPTPRVAEIMGPVRDVLSRIGMEIVASHSFEPASSHQPIVLALS
                     DVGEVVFLPAILRELRRLAPATSLRSVSLPAEEVARGLEAGTIDLALGYFPNLKRHNF
                     FQQTLFSDSFTCLIRADHPVKSPRLSSRQFLQLDHAVVRAESRTEEVIERFLARRRIK
                     RRVTLTTPHFASVPPIVEQSDLVVTVPVPLARYFSTVSARIRVVRPPFDPPRIDLKQI
                     WHRKFHHDARNRWLRGLMCALFQKPPAES"
     gene            complement(16570..17778)
                     /gene="mhpT"
                     /locus_tag="VEW08_15835"
     CDS             complement(16570..17778)
                     /gene="mhpT"
                     /locus_tag="VEW08_15835"
                     /inference="COORDINATES: protein motif:HMM:NF008586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HYJ42264.1"
                     /translation="MSTRQNVAVAVGLCFLVAVLEGFDIQAMGVAAPKLAPLFALDAS
                     QMGWVFSISNIGLVIGASVGGRLADWSGRKPVLLGAVAVFGAFTLATAFAGDYPTLLG
                     VRFCAGLGFGAALPNMMAIAAEISRPEKAATTAAAMFCGMPFGGGLAALTTQLLPADF
                     NWRTLFILGGVLPIVLIPALLRWMPETLVRGTSPRTHGTSVAQVLFSGGRATPTLLLW
                     LAFLPTLLILYLFLNWLPTLVIAKGLERAVAPQASLAFNFGSVAGALVCGWMVDRFRF
                     RWPLTLAYLLLIVLLFLLSAADSHAMLITLSGAVGFCLLGANYALYGVAPAYYPLSIR
                     GTGSGASIAVGRVGAIAGPLLAGLLISGGTSAAGVVQYMVPVAAVSAIAVFLLSFRPA
                     ASETGRTSGP"
     gene            complement(17775..19592)
                     /locus_tag="VEW08_15840"
     CDS             complement(17775..19592)
                     /locus_tag="VEW08_15840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HYJ42265.1"
                     /translation="MNSDAAHWTPDPFATQFERGDDGSLILRPLGDLAPYPQTIASSL
                     EFWARAAPDRMLVARRDAAGVWRQVSYAQMLARIQRVAAGLLARNLSAERPIAILSGN
                     SIEHLTLALAAMWVGIPYCPVSPAYSQVAGDLAKFRYVMDLLTPGLVAAFEPARFERA
                     TSLVPRGVEIVITLESLEQAQASGVEQAHAATGADSIVRFLLTSGSTGQPKAVICTNR
                     MCCSNAGMLRQSMPFLASEPPVLLDWLPWNHTFGGSHNLGLVLANGGSLYIDDGRPTP
                     AGISETLRNLREISPTVYFNVPKGFEMLASHLGKDPQLRKNFYRRLRAYFFAGASLAQ
                     HTWDELDDASSRELGFKVPMLTGLGATETGPSVTFTTPAMARSGAIGLPAAGTLVKLA
                     PVEEKLELRVRSPSVTSGYWRQPELTAQAFDAEGFYRLGDAVKLIDPAQPTRGLRFDG
                     RIGEDFKLANGTWVSVGPLRADLIGALAPAAQDVVIAGLDADFVAVLIIPDVDACAAL
                     IDCPCTVTRTELASDSRILTWIQQRLAAHARANPATSRGVQRARILPVAPSLDRGEIT
                     DKGSINQRAVLRHYAELVAELYSAAPPAHVVIIEREEST"
     gene            complement(19594..20463)
                     /locus_tag="VEW08_15845"
     CDS             complement(19594..20463)
                     /locus_tag="VEW08_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ42266.1"
                     /translation="MNIQDLVAIDVHTHAEVSCRHEPDEEWKKFEDASAKYFKAGKRP
                     TIPETIAYYRERKIGLVMFTVDSEFEIGARRIPNEEVADAARENSDIMMAFASIDPHK
                     GKMGVREARRLLEDGVIKGFKFHPTLQGFFPNDRMAYPLYELIAEHKLPAIFHSGHSG
                     MGTGMPGGGGLRLKYSNPIHLDDVAADFPDMTVIIAHPSWPWQDEALSVCLHKPNVYI
                     DLSGWSPKYFPPQLVKYANSQLKDRMLFGSDYPLITPDRWIKDFHEAGFKPEVHDLIL
                     KQNAIRALGLERA"
     gene            complement(20460..22565)
                     /locus_tag="VEW08_15850"
     CDS             complement(20460..22565)
                     /locus_tag="VEW08_15850"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HYJ42267.1"
                     /translation="MSASQCVRLERNGELALVVAHNPPVNTITAEVRAGLREVLDAIR
                     SARDIRAIILMCEGSTFFSGADIGEFSGPPREVEYRTLFNAMEALEVPVIAAMHGTVM
                     GGGLEIALASHYRIAAPTTRFALPEVTLGIIPGAGGTQRLPRLIGAERALDFILSAKP
                     VGAPQAMELGFLDQTIEGDLKTGACDYARSLLSAGKGPRRTGEMRVDPATASDEMFQR
                     LSDQARKLYPNRSAGLVAVEAVRKAAHSSLSEGLEYETALVNERKTSVESRGAIHVFF
                     AERESRRVPGLGDDIRAAPIKSAAVIGAGTMGGGIAICFANAGIPVTLLDANAAALER
                     GLANVTRTYQSMVERGRLTAAEAGQREKLIRTTLDYGDLGEADVIIEAVFESMDLKRE
                     VFTKLDAVARRGAVLATNTSTLDIETIANATSRPADVIGMHFFSPANVMPLLEVVRTT
                     HTSPVAIKTVMELAKPLRKTPVLAKVCYGFVGNRMMEGYAREAQRIVLEGATPRQVDS
                     ALEDWGMAMGILAVFDMAGIDVGVNVHRANASQYPPDPAYYQADVALHDAGRLGQKNG
                     KGYYRYEAGKRARLDDPEAIEILRRKSRELNVPARAHSTEEIQERCLFPLMNEGFRIL
                     GEGIVMRAADIDVVWTSGYGFPRYRGGPMFHAEVIGLDTLLKGMRRYRDIFGPMHWEP
                     APLLVELVEKKMTIAEWES"
     gene            complement(22568..23053)
                     /locus_tag="VEW08_15855"
     CDS             complement(22568..23053)
                     /locus_tag="VEW08_15855"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYJ42268.1"
                     /translation="MPRESNLKRKELPSEFEVPELMFAELDSLLGYTLRRAQGAMHRD
                     FMAAVSDFGLTQKQAAVLWLIQANGGVSQVEVAAALGMDRATMMALTDRLEDRGFVIR
                     KRSTVDRRRQELYLTPSGQSTLRKCKTRIAEHEEKFRAMFTASELAALIDALKKFQNP
                     V"
     gene            23165..>23567
                     /locus_tag="VEW08_15860"
     CDS             23165..>23567
                     /locus_tag="VEW08_15860"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYJ42269.1"
                     /translation="MEMFGATSRSITKRHKIAGCLTLAMPVLLLNAGPAAAQTPPDTT
                     GAEPEAEGLEAIVVTARRREESLQDTPVAVTALSADALERQQIVSTTDLDKVAPNIQF
                     HSYGTLTGNNSAAQVFIRGIGQTDATPAVDPG"
BASE COUNT         4351 a         7660 c         7235 g         4321 t
ORIGIN      
        1 gacgtctaag tttcgtgagc gcaccttcgt gcgcgcccag gaacttaagc gtgtttgacg
       61 aaatgcctgc cgtcgccgac gagcgacaga caaccagcgc cccacgcgaa tacacccgcg
      121 ctcctccgga gtaccgggtc attccttgcg gcagaggcga cgcgcgtcgc gcggaagccg
      181 aggctttcat ccgcaagcgt tttctgcgca gccacggcgc ccacatcgca accttcatgc
      241 cgacgcttat gttactgacc ggtgtcgaca gcgaactcgt cgcggccgcg ggtttccgga
      301 gcgctgcgca ggagccgctg tttctcgaac gatatctgcc tctcgccatc gagcaggcaa
      361 ttgcgtcgca atcaggcgcg cccgtgcagc gcgcggaaat cgtcgaggtt ggcaatttcg
      421 cggcactgga ttcccgccgc gcgaagattc tcatgtcgtt catgcccgcc tattttctcg
      481 aaggctccgc ccgttggctc gtgttcacgg ccacgtcttc catccgcagg atcctctccg
      541 cgatgggcgg gcgctgcctg gaggtgggca ccgccgatgg cgcctgcgtc gccggaggca
      601 ttgatgagtg gggccgctat tactcgaccg acccgcgcgt gatggccggc ttcctgccat
      661 tggcgagacg catcccggaa ttgtggagga cgcatcatgg aaattgatcc cctgctcgcc
      721 gagacgccgc ggctcacgcc ggggcggatc gcgctcaccg atggcatccg ttcgatcacc
      781 tacggccagc ttgggtcgat cgtgcggacc gaagccgatc tcttgcgcga ggccggcggc
      841 ctgcgtttcg gactgctcgc ggaaaacggt tgcgactggg cggtcgcgga tctcgcggtc
      901 catcacctga atcgcgtaaa tgtcccgctg cccgcgtatt tcaccgcggc gcagatgcgc
      961 catgcgatcg acgatgcagg cgtcgacacc ctgatcaccg accggcgcga tgaagtcctg
     1021 gacagatggc cggagttctc ggtggcaggc acgttgtcgt cgaacggact ctccgtcctg
     1081 cgtcgtgcgg tggaacctga gcagcggcgc gcggttccct tcggaaccac caagatcacg
     1141 tatacctctg gcagcacggc ggacccgaag ggcgtgtgtc tgcccggcgc agcactggac
     1201 cgcgcctcgc gcgcgatgat gcagatcact tcgccgatcg gcatacgtag gcatctgtgc
     1261 ctcatgccac taccgacgtt gctcgaaaat cttgcgggca tccatgctcc gctgcgtgcc
     1321 ggcgccacct gtgtcgtccc ttcgagcatc gacacgggca tgagttatgg cgcgctggac
     1381 cctcaacgcc tgctcaggtc actcacgctg caccgcccgg agagtctggt cctggtgccc
     1441 gagttgttgc ggctcatcgt gcgcgccgcg caaggcggat acgcgctgcc ggactcgctc
     1501 aagttcatcg cggtgggcgg agcgtctgtc tcgcgcagcc ttctcgatga agccgccgcg
     1561 ctggggcttc cggtcttcga aggttacggc ctcagcgaat gtggctccgt cgtctgtttg
     1621 aatactccgc aggcgaagcg cctcggcagt gtgggccgcg ccctgccgca cgtcagcctg
     1681 tcgctcggca gaggtggtga gctgcgcgtc tcggggtggg tcatgtccgg ctatctcggc
     1741 gactcgcaga ccgaagggcc gcacgagatc gcgaccggcg atctcgcgga gatcgatgcg
     1801 gacggttacg tctacatccg tggccggctg cgcaatatct tcatcacgag cttcggtcgc
     1861 aatatgtcgc cggagtggat agaacgcgaa ctcgcacacg aacccgcgat ccgccacgcg
     1921 ctggcgatcg gcgagggatg cccggacgtg cgcgccctcg tatgcccggc gaaaccggag
     1981 ctcgagcaga ctgcaatcga gcacgcggtc gagcacgcga acgaacggct gcccgactac
     2041 gcgcgcgtgc gccatgtcgt gcgcatgccc gaggcaccca ccatttcgaa tggcttgctg
     2101 accgccaacg ggcggctgcg ccgcgagcga atcctcgaaa gatttggtgc cctgctggaa
     2161 caagccgcct gaatacaccg gagaaccctt gtctaacttc actgaacttg ccgctcgtac
     2221 ggcatccgcc cggacacaac tgcagacgac tccagtcatc gcgcgcgcgt tgcgcggtga
     2281 ggtgacgcgc gagttgtacc tggacttcct cacgcaggcg tatcaccacg tgcgacacac
     2341 cgtgccgttg ctcatggcgg tgggcgcccg tctgccggcg cggcagctgt ggctgcagaa
     2401 ggaactcgtc cactatctgg aggaggagct gggtcacgac gagtggatcc tttcggacat
     2461 cgcgaccgcc ggcggggatg ccgatgcggt tcgcaacggc gcaccatccg tcgaaactga
     2521 tgcgatggtc gcctatgcct acgacacggt gatgcgccgc aaccccgtcg cgttcttcgg
     2581 catggtctat gtgctcgaag gcacgagcgt ggccctggcg ctcaacgccg ccgacgccgt
     2641 ccagcgcacg ctggcgctgc caaacaaggc gttcacttac ctgcgcagcc atggctccct
     2701 cgaccaacag cacatcggtg acctcgagag tattgtcgat cgcttcgacg cgtccaccga
     2761 cctgccggcc gtcgtgacgt gcgcgcgcgc catgttccgg ctgtatggca acgtgttccg
     2821 ttcgctcgaa tccggagaaa ccgcgggcgc gatgctgctc cagggggccg catgaaactc
     2881 gatgtcatgc gcgtactgct caccggcgcc accggcggaa tcggcagcgt catcgcgaag
     2941 cgcctgctcg aagaaggcgc ccacgtgttg ctcgtcgctc gcgatgaggc ggcgctcgcg
     3001 gcactcgtcc agtcatgctc attcgcgagc gatcgggtac aggctcacag tgtcgatatc
     3061 acgcaaagca aatcacggac cgcgctttgc gaggtcgcgc ggcaatggca tggcggcgtc
     3121 aacgtgctca tcaacaacgc cggcctcaac accttctcga tgtacgagga gctgaccgag
     3181 gatcagatcg accagactct cgccgtcaat ctgcaggctc ccatgcacct gtgccacgat
     3241 ctcctgccgt ggctgcgcac acgcgagtcg gcggccatcg tcaatgtcgg ctcggtcttc
     3301 ggctccatcg gcttgcctgg ctacgtggct tattccgcca ccaagtttgc catccgcggg
     3361 ttttccgaag cgctgcgccg ggaactcgcc gacagccgcg tcatggtgaa acatcttgca
     3421 ccgcgcgcca cgcggaccgc gatgaacacc gccgcggtcg tggagatgaa ctcacgtctc
     3481 aaggtcgcga tggatccgcc cgagcgcgtc gcgcgcgagc tcatcgcact attcaagcgc
     3541 cgtcgcagtt cagcagtcgt cggttggccc gagaaggcct tcgtcaagtt gaacgccgtg
     3601 cttcccggca tcgtcgaccg cgcggtgcgc ggccaactat ccatcgttcg cgcatttgcc
     3661 cgccgcttgc gcccccagga gatattttcc aaatgaacat caagactacg ctgcacaact
     3721 ggttagagac attggcgata gccgtcgcgt tcctgccgct cgcgttcccg gcgcttgccg
     3781 aggatgcccc cttcaccgcg gagctcacgg gcatccagca aagctgggac caggccaact
     3841 accgatccgc gaatgcggaa gacaaacagc ggcagctcga agctctgtcg gcgcgcagcg
     3901 aagcgctcgt tcacaagtat ccgcagcgcg cggagcctct cgtgtgggaa ggcatcgtgt
     3961 tgagcaccta cgcgggcgcc aagggcggcc tcggagcgct gtcgctggcg aagaaatcgc
     4021 gcgactgtct gctcgaagcc acgcgaatcg atgccgccgc gctacggggt tcggcataca
     4081 cgagcctcgg tgcactgtat ttcaaggtcc cgggctggcc catcggattc ggcgatcgcg
     4141 acaaggcaac caccttcctg aacaaggcgc ttgcgctcaa tccggacggc atggacccca
     4201 actacttcat cggcgagttg ctgtacgagc agggagagta ttcccgctcg ctgcaacatc
     4261 tgcagaaggc gctggcggcg ccaccgcgcc ccgatcggcc cgtcgccgat gcagggcggc
     4321 gggcggagat caacacgctc atcgcaaaag tgagctcgaa actccagtga gagctgcatc
     4381 ccggcgctca acttcctgac gaagtacggt ggcgcgcgcg gttccgggcc tagggttgca
     4441 gcgtcgtatt gaacaggtat tggctgtcga actcagtctt gtcgccggcc ttcttgccct
     4501 tgaccggttg cagtcgcgcc gagagcggct tattccattt ccacgccagc gccgcgacgt
     4561 cgccgaagat tttcgcgaga cgttcttcgc tgacatcacc gggcaacgga acggtatcga
     4621 gcccggtccc gcacaccgcg gaatacgcga gcacggaatc gatcgcatag gtgttctcgg
     4681 cccagcgttg cgcgagacgc ttgtcttcca tcaccggcag catcagtccc gaatagccga
     4741 cctgcttcac tttcactgcc ttgacggccg acgtaatggc gagcaccgca gtcatcgtgc
     4801 cattcgcgcc gaacttcgaa cccgtgtagc gctcgatcgc atcgccgatc gacgtatcgc
     4861 cgagtggcgc cggcgtggga tccacaccgg caaagctcca gccagtggcc gcggcgacgg
     4921 cctcgcctac ttgctcgcca accctggcgt ggatcgtcaa ctgccgtgcg agctccgcca
     4981 ccgacgcgtc gaaatcaccg tgagtcacgc cgaatacttc ctgcacgacg ttggcgcttt
     5041 caaatcccaa cgaaaacagt tttccggcgc cagtgtgata agcacccgga tagaaaggcc
     5101 cgagcggctt gagcatggcg gttgccgtga actgaaaatt gccctggctg cgcggactgt
     5161 tgtccgtcag gtagcgcacg agctttgccg actcacggat ggtcttccag tggataccgt
     5221 cgtcgtcagc aatgatggcg ctcgcattga gattcggcat cgtggaaagc acccgcgcca
     5281 gcagatgcat cgcggcggga tcgtccgtgt cgtgcaacat cgccggaccg acattcgccg
     5341 cgaaaccggc cttttgcgac agttcgtcga atgagcggag gaacgcgagc gcctctttgt
     5401 ccgggaggcc cttcaccagc tccgcgaatg gctgggtcac gatgcgaacg gtttgcgtct
     5461 cgtagccacg cttcgcgaag tcggctttcg cggcgttgag caccttcatc gcctcctcga
     5521 cctgcttttc gtaggtcgag cgatcgaggc gtacgaacgc cgtaatcgcc cgcacgttcg
     5581 gcttcgaggc atcaggtgcg gcgagcgctg tggttgaaac gagcgcacag gacaggacca
     5641 aggcgatcag gtttttcatg gccacatctt acataggccc ttcagcaggg gttcagttgt
     5701 cgccgacgac aatggcagcc gtccctctct cactttggag ggagacgcat ccacttctac
     5761 gggggatgcg tactttgagc tgaccgcgca ttgttagcgc caggatctga aggagcccaa
     5821 catgaagcgt cccaacccaa aaatgcacat gccgcagcac gactacggtc aggcactgca
     5881 aaccgccgtg tcatggctgg gcgagcgcta cctgctggcg gagcccgtat cgcggcgcat
     5941 cgaggagccc aagccgttct tcgtcgtgca gcagtcctgg cacgagccgc ggcgcaccgt
     6001 cggaccgcgc agccgaaggc actgagatgc ccgctccggg ttagtcaccc gaaaacgtcc
     6061 gggctggtac gtcagtccag cgcccgtgct agaaggcctt gccagcgcat gtaatatgct
     6121 ctgcgccacg gcaacttcat gagcacggaa ccatgaccat ccgaaacttc gcgccccgcc
     6181 cgatgaagct cagcgtattg accgctgcat ttcaggagct gacaccgcgc gatcggcgcg
     6241 accctgatcc ggatctggcc atcgaagaat ggctggagtt cgcccgggac atcggctcac
     6301 ccaacatcca gctttcttcc gcgctgcatc cgactttgag cgatgtcccg cccgaagcaa
     6361 tgctcgaccc ggtggccaac acgctcgacc tgcgcgcgcc tttcgacaag agccgcgcgg
     6421 cccgcgtgct gaaggccatg aaggccaccg gcgtcggtct ctccgacctg ggctatttcg
     6481 acaacatgct cacggacgac gtgaccgcgc gcaaacacaa gcacgacttc atgttgcggg
     6541 tgttcgatgc cgccgcgctg ttgggcgcca acgcagtatg cggtttcgtc ggccgcaata
     6601 tccggctcga aatggacgag aacctcgtca tgttcgagca gcagttcata ccgctgctca
     6661 aggaagcgaa ggcccgcggc ctcacctacc gcgtcgagca atgccccatg ccggggtgga
     6721 atacgctcga ccgctggcac aacaacatcg cgtacgcgcc gggtccctgg atcgccctgc
     6781 atcgcatctg cgagaaacac ggcgtcggtg atcagttccg cattcattac gatccatcgc
     6841 acgccatcct catgggacag gacacccgtt cgctgttcca gtacctcaag gatgagggct
     6901 atggattcct gatcggtggc ttccacgtca agggccaggt cgtcgactcc cgcggcgtct
     6961 ccgcgtgggg ctatggcggc cagacgatgc agcgcggcga ctggaaagac ggcaagccct
     7021 ccaccaaccc cgcggacgcc gccaacgcgt ggaagaagca ggtggtgctc gccgaacacg
     7081 agctgcccgg caccgcgcgc cacgatcccc tcgcctatct gcagaatcgc accgtcgatt
     7141 ggctcgacca ccagcttgcc gcgcgcgagc tgttgtcgat cgaccccgcg gacacctatc
     7201 tagtcgtcga gcacgagtat ccgccggctc gcatccagga caaggcccgg ctcgctccga
     7261 tcttgaaagg ctcgctcgcg ttcgtgaagg ccatagacga ggccgccgcg gcgatgttcg
     7321 cgctccagca tgaggtgatg gcggcccagg gaattccaat ccagggcaag ggccgcgagg
     7381 cgtatcgctc gtgattggag cccggctgag cgcgatgatg ttcctgcagt tcttcatctg
     7441 gggctgctgg ttcgtgactc tgggaacgtt tctcgcggcc aacttccagg cgagcggcgc
     7501 acaaaccggg ctcgccttct ccacgcagtc atggggcgcg atcatcgcgc cgttcatcat
     7561 cgggctgatc gccgatcggt atttcaacgc cgagcgcatt ctcgcggtgc tgcacctcgt
     7621 cggcgccgtg ctgatgtacc agatgttcaa ggcgcaggac tttgcgcagt tctatcccta
     7681 tgtgctcgcg tacatgatcg cgtacatgcc gacgctggcc ctggtcaact cgatctcgtt
     7741 ccgccagctc accgatccat cgcgacagtt ctcgctggta cgcgtgtggg gcacggtggg
     7801 ctggatcgtc gccggactcg tgatcagctt cgtgttccat tgggattcgc aggaggccgt
     7861 ggcaagcggc gcgctgcgca atacgttcct gatgggcgcg gtgatgtcgc tgctgctcgc
     7921 cattttcagt ttgacgcttc ccgccacacc gcccacggta gccaagggcg ggttttcgct
     7981 tcgcggcgcc ctgggactcg atgcgctgaa gctgctgcgc gaccggaact tcgccgtgtt
     8041 cttcgtctcg tcggtgctga tctgcatccc gttggccttc tactaccaga acgccagccc
     8101 gttcctgacc gagatcggca ccgcgaatgc caccggcaaa ctgaccatcg gtcagatgtc
     8161 ggaagtcgcg ttcatgctgt tgttgccggt cttcctgctg cgcttcggca tcaagaagac
     8221 gctgctgtta ggaatgctcg cctgggcggt tcgctacgtc ctgttcgcct atggcggcca
     8281 ggacaacatc tccgtgctgt tgataatcgg catcgcattg catgggatct gctacgactt
     8341 cttcttcgtc tcgggccaga tctacaccga ttcgaaggcc ggcgagcatt accgcagcgc
     8401 cgctcagggc atgatcaccc tggcgactta cggcgtcggc atgctggtcg ggttctcctt
     8461 cgccggaaag atcgctgatc aattcaacgt cgacggccag catgactggt ttcagatctg
     8521 gctctaccca gctgggattg ccgctgtgat cctggttttg ttcgcgttgc tatatcgaaa
     8581 cgagtccatc acctatcaaa agccggatct cgattcccga cgcccaagca ggtaatgtgc
     8641 gggccgcatg ccacgcgcca agccccgctc ccgcaaaccc tccccacccc gagggtactc
     8701 ggcacccgcg ctcgagaagg ggatggacat catcgaattg ctggccgact cggaaaacgg
     8761 cctttcggtg tcggagattt cgctccggct gaagcgtcgc atgagcgagc tgttccgcat
     8821 cgtcgtcgtc atggagcggc ggggctggct gcaaaaggat ccggagacat cacgttactg
     8881 cgtgacgtac cacgtactga agctcgcgca ccgtggcact ccgacacaaa cactgacgtc
     8941 cgcagccgct cccgtgatgc aggacctgtc tacgcgcatc aaccagtcct gccacctcgt
     9001 ggttcgatcc ggcacgcagg gcctggtgat actgcggcag gaaaaccagc tgcggcatgc
     9061 aaacctgtcg gtcaggctcg gcgcagtcat cgacctcgtc acgagctgtt cggggcagat
     9121 tctgctggcg cacatggaac ctgcggaacg ctggcgcctg ctgcaatcca tcccgtgccc
     9181 gcgcggaacc tcgaaggcga aagtggatgt cgaactcgaa aaagtcagga agcggggatt
     9241 cgaaattcac cgtagcccca ttactgcggg cgtgaccgac ctgagttatc ccattcgcga
     9301 tttcgacgac aaggtggtgg ccgcactcac tgttccctat ctgcacgcgc tggataactc
     9361 actgccgacc tccgtcgaac aaacgcgcca tctgctcgaa gaagccaccc gccggatatc
     9421 gaagtcgctc ggcgcaacgc gataaccggc gggcgccgat ctatttcttc ttgaacgcgc
     9481 gtgacgcgaa gtccttgagg tttcgccgcc aggtttgcca ctcgtggtcc gttccgggcg
     9541 actcccagta cacgtgatcg attccggcct cggtcagcgc ggcatgcagg ccgcgaattc
     9601 gcgtgctgaa gcgctccggt tcggcggttc ccacgccgaa atacagcaga tgaactttct
     9661 tcgcgaacgc ggccggatcc gcgaacatgc cgttgaaatc ggttttgaaa tcccacttgc
     9721 gatcttcgag catcccgccg atgccactga agccaccgat gtgcgagaac agatcgagat
     9781 gcctcgtcgt gatgtggaaa gtctgaaaac ctcccatcga taaccccgcc atgccgcggt
     9841 tatcgcggtc cgacagcgtc ctgaacgatt tgtcgacaaa gggaatcagc gcctgcgtga
     9901 cgtcgtcttc gaacgtcttt gccatctctt cgcgcgcttg cgcagcctgc ggcgaacccg
     9961 gggtcggatt cagcagcggc gccccggcgc ggcgcgcata gccgtaagcc atgacgatga
    10021 tcatgggttt cgcctcgccc gcggcgatca ggttgtcgag aatgacattg gcgcggccct
    10081 ggcgaatcca gcccgtttca tcttctccac cgccgtgctg cagatagaga accggataac
    10141 gcttcttcgt ttgcgcctcg tagtccggcg gcaggtaaac cattgcatgg cgccacgagc
    10201 cggtgacctt cgactcgtac cacacgtcgc gaacctggcc ttgcggcacg gcctggatcg
    10261 agtaataggt agagccgggc tccggaattt ccaccgcgct cacgtatcgt gagccgccga
    10321 agaaggctct gctccccgga tcgctggcat cgacgccatc gacgttgatc acgtagtagt
    10381 gaaggcccgg caccagaggc gtggtggtga tggtccagaa tccatccggc tgtttttcca
    10441 tgtcgacctt gggcccgctc cagaagttga gcttgacctt gctggcttcc gccgccttga
    10501 tgcgaatctg cacccggccc gtggcgtcga tgcgcggata ctccgcgccc cagacattcg
    10561 tctcggcagg ctgaaaccgg ctcgcctcct ccgcgacaca ggtaccggcg accaacgcca
    10621 aagcacagac gagcatcctc atgtttccca cctcacattc aagcttgtaa aaaagaccga
    10681 ggctgccgtg caggggaacc ggcacggcag cctcgccggt gactgcgctc gaaagcgtta
    10741 gcggaaacca gtcaccactc gacccacggg acgcgagacg gttgttgtca tcgcccggcc
    10801 gcacccggcg ctggcgtctc ccgacccagg gtgccctaac taatctatcc gcacgcaact
    10861 aggctcacgg cggccccgag caatgtcaag caatagttgc ctgccatatg cgttttgcta
    10921 ttcatcgatt cgtttagtaa aacttccttg ccatagtgaa agccggttac ggatggtacc
    10981 ttctgcaacg aaaccggatc gaagggggat cccatgtcga acaccttgcg ggcgcttgcc
    11041 ggcgccctcg cctgtttttt cctcgccggg acgggcgttg cggcggccgc cgatgaaccc
    11101 gtcgagattt cagtggatgc ggccaatccc ggggccgcca tcagccgcca cctctttggt
    11161 caattcgcgg aacaccttgg ccggggtgtt tacgaaggga tctgggtcgg ccccgattct
    11221 ccgattccaa acacgcgcgg aattcgcaac gacgtcgtcg cggcgctcaa ggccatcaag
    11281 gtgccgaacg tgcgctggcc gggcggctgc tttgcggacg agtatcactg gcgcaatggc
    11341 ataggacccc gggaccgccg ccctgccacc ctgaacccga actggggcgg ggtcatcgaa
    11401 cccaacacct tcggcagcca cgagttcctg gatttcgcgc agcagattgg tgcggatgcc
    11461 tatctatcca tcaacgtggg ttcgggcacg gcggccgagg ctgccgattg gctcgagtat
    11521 ctgactacgc cgcagcccag tgcgcttggc aaggagcgtg cggcgaacgg gcatccggag
    11581 ccgtatcgca tcgcgatgct gggcatcggc aatgagtcct ggggatgcgg cggctcgatg
    11641 acgcctgatt actacgtgag ccagctgaag gtccacgcgc gttatgcgcg caactacaac
    11701 gccgcgcaga agatgcagcg tatcgctgtc ggccccgatg gtgcgcacac cgagtacacc
    11761 aaggcagtga tggaggcgtg gaagaacaag acctgggcgt gggacatcga gggaatttcg
    11821 ctccacttct acacggtggt gaagtggcct ccggcattca aagccacggg attcggcgaa
    11881 ggcgagtacg ccacattgct caacgacacg cggcgcatgg acgacctgct ccggactcac
    11941 ctgtcgatca tggatcaata tgaccccgcg aaaaaaatcg cgctgatcgt cgatgagtgg
    12001 ggcgcctggc tggcgccgac accgggcagc ccggaaggct ttctcgagca gcaaaacagc
    12061 cagcgcgatg ccctcatcgc cgcattgaac ctcaacatgt ttgcacgtca cgcggaccgc
    12121 gtgcgcggcg ccaacatcgc gcagatggtc aacgtgctgc aggccatgat cctcaccaac
    12181 aaggacaaga tgctgctgac gccgacctat cacgtgttcc gcctgtacct gcccttccag
    12241 gacgccacgt tgctgccggt gaaagtgtcg gccggggatt atcagcacgg cgacatcaag
    12301 cttccacgcg tcgatgccat tgccgcgcgc gacgctcaag gcaagacctg gctggccgtt
    12361 tcgaacctcg atcccggccg ggccacccag gtctcgctcg atgtgaccgg cgcgaagatc
    12421 aagcgcgctc gtggcgaaac gctcacctca ccgcgggtag acagcgtgaa cacatttgat
    12481 gccccggaaa cggttgcacc gaagcctttc gaggcccggg cgtccagtgg acggttgaag
    12541 ctttcgctcg cgcccgcctc gatcacggtc gttgcgctgg agtagccggg agatggcact
    12601 caaggctcga ggctccttgc gcgtccacgg aaccatcgcg cgtgaaattg gcctgtcgat
    12661 cgtgtcggga agacttcggc cgggccatgt gctggacggt gagatagaag ccagttcgca
    12721 gttgaaggtt tcgcgcacgg cctatcgcga agccatccgc atcctgagtg ccaagggtct
    12781 gatccgaagc cgcccgcgca ccggcactcg cgtgaacgca atctccgact ggcacttgct
    12841 cgacccggat gtgctcacgt gggtgttcac ccaggtgccg aaacccgaag tgattcatgg
    12901 attgttcgaa ttgcgcacca tcgttgagcc ggcagcggcg gcgctggcgg cgaccaggcg
    12961 cacccggatt cacctggccg aaatgcggcg tgccctggac gcgatggcgc tgcacaccct
    13021 ggacaaggtt gaaggccggg aagcggacaa ggcatttcac gccgcgttgc tggcggcgac
    13081 ggcaaaccct ttcgtcattt cgctgaccaa cggtgtcacc gccgcggtca atgcgctgac
    13141 ggagttcaaa cagcggttgg caaaaatcga gcgcgatccc gtgcccgatc accttcacgt
    13201 cttcgatgcc atcgcggcga aggacgccga ggctgcgcgt gaggccatga tcaagctgat
    13261 tcgactcgcg gtgctcgaca tgaacgtcaa acaacgaccg cgtccgcctt ccggcggaaa
    13321 gttgtccgag gctgcgtatt tcataatggt gtagaaggcg ggaccacctg cgcttcgacc
    13381 aactccgcct gctggcgcag ccacgcagcg gtgcgaagaa atccgccaaa agagtacagg
    13441 tgaatcccgt cgaagaggtc gggctggcgt cgtcgttccg ccgccagatc gtctaacaag
    13501 acgccggggc tacgctcgga caacagtttg aacatcgatc ggtagcgcga agacagcacg
    13561 cgaatcgacg gccccacacc acagtgcctg gcgagcgcga gcaggcgtcc ggccgtggcg
    13621 gggcccggga ggccggcgac gatgcgcgct tcaacgcccg cccgccgcag gtcacgggcc
    13681 caatcgacga acggggtggc ctcgaaaaag aactgtgtca ccagcgtgac ctgcagtccg
    13741 gccgccacgc cgcagcgcgc cttttcgatc tgcgcgcgcc ggatttcatc ggccgccacg
    13801 gccggatggc cctcgggatg gcctgccacg gagacgcaag tgaatccgaa tttctgcaac
    13861 ttgccggttc gcatgacatc caggacttcc cggtaaggac cctcgggcct cacgtagtcg
    13921 cccgatatga gcagcaggct gctcgctccg gcatcacgga tggccgacag aatgccgtcg
    13981 agctgctgct cgttcttcag aagtctcacg ggaacgtgcg gcacgggctc gaatcccgcg
    14041 gccgcgacct gggcacaggt attcacggtc tgcgcctggg tctggccggg cagatgactc
    14101 acatagatct tctgcctggg ccagagaaaa tcgcgacccg cgcggatgtc ggcggactcc
    14161 agcgaattca cctcgatgga agactcgcgc gcgagtccct ctctcatcgc ggcgaggtcc
    14221 gccacccggc gtgataggaa tcacgcgcat cgcgcgaata cggtacgggc gacaccttga
    14281 cgcgaacttc acactggcgg tgacgctcga cagtgggctt cgccgtgcct ccgtcctcct
    14341 cgccccagac cagtgtgagt tccgtcccgg cttcgatatc tgcatcgacg acgcccaatg
    14401 aaaggccgca tcgctcgttg tgactgtagc cagtgaacat cgagaatccg acgagccggt
    14461 cgccgagttt caccctgtcg taagacgacg aggcgtagtt ggcgatcggc aggtcgaaga
    14521 acttgtatgg caggttctcg cgatcattca aggaagcgag aattctgccg agatcctcgc
    14581 cgttccattc gaaggtcacc ttgcgacggt gcggatctga ctcctgcacc cgcttctcga
    14641 gcgcctccct tccgatgaat tcatgatcga acttcacgaa ggatccgtag cccagttcgt
    14701 aaggcgtcag gtagtagtcc tcgatattcg ccgacacgaa gcttccgccc agcgagccgg
    14761 tgccttcgta cccgtttgcc ggcagccact cgcggtacgc gcgcatcttt tcgcctgtgt
    14821 agaccgcggg caacggcgat ggaatccatc ccgactcgag cgtattgctg gcgtaggttc
    14881 gcgatccgac agcgacgatg ccgaattcct tgccagcgtc gaggatggcg gcgcgcatct
    14941 cgtcgcgctc ttcatagggc ccgaatatct cgaggcccgg ctcaccggcc attccatggc
    15001 gcaacgcacg caccctgcgc ccggcgatgt tgagccactc gaagttgaag aagcggatgt
    15061 ccggaagcgg cccgccgtac aacttctgca gaacctgcgt cgcacgcggt ccctgcacct
    15121 ggtagcgaaa atggcggcgc ttgaccgcgc ggccattggg ttgcgaaggc gatcgatcgt
    15181 cgcggatcac cttcacgtcg aagcctgccg tctcggcgtg aaattgcagc cagttcaccg
    15241 tgggcgctcg gcccacgaac agcagctcat ccttctcgag gtagaacagg atgccgtctc
    15301 cgatcacgaa tccatcgtga ttgacgggaa tcatctgctt ggccttgccg ggcgcgaagc
    15361 tcgcaaagct gttgatcgtc gtatgcgagc aaagccgcag ggcatccgga ccctggatcg
    15421 tgatttcggc catgtggtgc gactggtcga acaacacggc gccgctgcgc caggccagtt
    15481 gctcgtcgcg ccagttgctg aactcggtcg gcacgacggg atagacatag gctccgatcg
    15541 gagaattgcg cagcatgcgc acggtatcgc cggccgaccg gatcaggctt tcgaggttgg
    15601 gatggttcat cgcaggccct ccgggcgcga tcatcgaagc gattgcccct atgtggcaac
    15661 gcgatatgat caattcactg tattcgattt ccggataacc gatgggcgag ctcgatttga
    15721 atctgctgcg cgtgctggtg gctctcgacg acaggcgcag cgtcagcgcg gcggccgccc
    15781 agctgcagcg cagccagccg tcggtgagtt cggcgctcgg aaagctgcgg gcattcttcg
    15841 ccgatccgct gttcgttcgc agcggcaact cgatgcaacc cacacctcgc gtggccgaga
    15901 tcatgggccc ggtgcgcgac gtgttgtcgc gaatcggcat ggagatcgtc gcttcgcact
    15961 ccttcgaacc cgcgagcagc catcagccca tcgtgctcgc cctttccgat gtcggcgaag
    16021 tggtgttcct gccggccatc ctcagggaat tgcgccgtct ggctccggca accagcctgc
    16081 gttccgtgag cttgccagcg gaagaagtgg cgcgtggact cgaagccggc acgatcgatc
    16141 tcgcgctcgg ttatttcccg aacctgaagc gccacaattt tttccagcag actctgtttt
    16201 ccgacagctt cacctgcctg atccgcgcag atcacccggt caaatcgccg cgattgtcgt
    16261 caaggcagtt cctgcagctg gatcacgcgg ttgtcagggc cgaaagccgg accgaagagg
    16321 tcatcgaacg cttcctggcg cggaggcgta tcaagcgtcg cgtcacgctg acgactcccc
    16381 atttcgcgag tgtgccgccc atcgtcgagc aatccgatct cgtggtcacc gttccagtgc
    16441 cgctggcccg gtatttctcg accgtgtctg cgcgcatccg tgtcgtgcgg ccaccgttcg
    16501 atccgccacg gatcgatctg aaacagatct ggcaccggaa gtttcatcac gatgcgcgca
    16561 atcgctggct caggggcctg atgtgcgccc tgtttcagaa gccgccggcc gaaagctgag
    16621 caggaagacc gcgatcgcgg agaccgccgc cacgggcacc atgtactgca cgacgcccgc
    16681 ggcgctggtt ccaccgctga tcagcaggcc cgccagcaaa ggtcccgcga tggcgcccac
    16741 gcgcccgacg gcgatgctgg ccccggagcc ggtgccgcga atcgacagtg ggtagtaagc
    16801 cggcgcgaca ccatacagcg cgtagttggc gccgagcagg cagaagccga ccgcaccgct
    16861 cagggtgatg agcatcgcat ggctgtcggc tgcgctcagc agaaacagca atacgatcag
    16921 cagcaggtac gcgagcgtca acggccagcg aaagcgaaac cggtccacca tccatccgca
    16981 gaccagcgcg ccagccacac tgccgaaatt gaaagccagc gaagcctgcg gcgccacggc
    17041 acgttcaaga cccttggcga tcacaagtgt cggcagccag ttgaggaata gatagaggat
    17101 cagcagcgtg ggcaggaacg cgagccataa caacagcgtc ggcgtggcgc gcccgccgct
    17161 gaacagcacc tgggccacgc tcgtcccgtg cgtgcgcgga ctggttcccc gcaccagggt
    17221 ttctggcatc catcgaagca gcgcaggtat cagcacgatg ggcagcacgc cgccgagaat
    17281 gaacaaggta cgccaattga aatcggcagg aagcagctgg gtggtgagcg ccgccagccc
    17341 gcccccgaag ggcatgccac agaacatcgc cgccgccgtc gtcgcggcct tctccggccg
    17401 gctgatctcg gctgcgatgg ccatcatgtt gggcaacgcc gctccaaacc cgaggcccgc
    17461 gcagaaccgc actcccaaca gcgtggggta gtcgccggcg aacgcggtcg caagcgtgaa
    17521 ggcgccgaac accgccaccg cacctaacaa taccggcttg cggccggacc agtccgccag
    17581 cctgccgccg acactggcgc cgatgaccag cccgatattg ctgatgctga atacccaacc
    17641 catctgactc gcatcgagcg cgaacagcgg ggcgagtttc ggcgccgcca cgcccatggc
    17701 ctggatgtcg aatccctcga gcaccgcgac caggaaacac aggccgacgg cgaccgcgac
    17761 attctgacga gtgctcacgt ggactcctcc cgttcgatga tgacgacgtg cgccggcggc
    17821 gccgcggaat agagctcggc cacgagttcg gcgtaatgcc gcaacaccgc tcgctggttg
    17881 atggaacctt tgtcggtgat ttcgccgcga tcgagcgatg gcgcgaccgg caggatgcgg
    17941 gcacgctgca ctccgcggct cgtcgcggga ttggcacgcg cgtgcgccgc caggcgctgc
    18001 tggatccacg tcagtatccg ggagtcgctg gccaactccg tgcgcgtgac ggtgcatggg
    18061 cagtcgatca atgccgcgca agcatcgaca tcgggaatga tgagcaccgc gacgaaatcg
    18121 gcatcgagcc cggcgatcac cacgtcttgc gcggccggcg ccagtgcacc gatgaggtcc
    18181 gcgcgcagcg gccccacgct gacccaggtg ccgttggcca gtttgaagtc ctcgccgatc
    18241 ctgccgtcga acctgagtcc ccgggtcggc tgcgctggat cgatgagctt caccgcgtcg
    18301 ccgagtcgat agaacccttc cgcatcgaag gcctgtgcag tcagctcggg ctggcgccaa
    18361 taaccgctcg tcaccgaggg actacgcacg cgaagctcga gcttctcttc gactggcgcg
    18421 agcttgacga gagttcccgc cgccggcagt ccgattgcgc cactgcgtgc catcgccggc
    18481 gtggtaaacg tgaccgaggg gcccgtttcc gtcgcgccga gacccgtcaa catcggcact
    18541 ttgaatccga gctcccgtga cgatgcgtcg tccagttcgt cccaggtgtg ttgcgccagc
    18601 gacgcgcccg cgaagaaata cgcccgcaac cgccggtaga aattcttgcg cagttgcgga
    18661 tcctttccga ggtgcgacgc cagcatctcg aagcccttcg gcacattgaa gtagaccgtc
    18721 ggtgaaattt cccgcaggtt tcgcagtgtc tccgagatgc ccgcgggtgt gggtctgccg
    18781 tcatcgatgt acagcgatcc cccgttggcc agtaccagtc cgaggttgtg gctgccgcca
    18841 aaagtatggt tccagggcaa ccagtcgagc agcaccggcg gttcgctcgc gagaaaaggc
    18901 atggattgcc tgagcatccc ggcattgctg cagcacatcc ggttggtgca gatcactgcc
    18961 ttgggctggc cggtcgaacc ggaagtcagc aggaatctca cgatggaatc ggcgcccgtg
    19021 gccgcgtgag cttgctcgac accggaagct tgtgcctgtt cgagcgattc cagcgtgatg
    19081 acaatctcca cgccgcgagg aaccagcgaa gtcgcgcgct cgaatcgcgc tggttcgaat
    19141 gcggcgacca gtcccggcgt gagcaggtcc atcacgtagc ggaatttggc gaggtccccg
    19201 gccacctggg agtacgcagg cgagacggga caatagggaa tgccgaccca catcgccgcc
    19261 agtgcaagag tcagatgctc gatgctgttg ccggacagaa tggcgatagg tcgctcggcg
    19321 gaaaggtttc gcgccaggag accggcagcc actcgctgga tgcgggccag catctgcgca
    19381 taactcacct gccgccagac acccgcggca tcgcgtcggg ccaccagcat gcgatccggc
    19441 gcggcgcgcg cccagaattc cagcgagctg gcgatcgtct gtgggtacgg cgccagatcg
    19501 ccgagcggcc ggaggatgag ggaaccgtcg tcgccgcgtt cgaactgcgt cgcgaatgga
    19561 tcgggtgtcc agtgcgccgc gtccgaattc atgtcacgcg cgctctagcc caagcgcccg
    19621 gatggcgttc tgcttgagga tcaggtcgtg cacttccggc ttgaatcctg cttcgtggaa
    19681 atccttgatc caccggtccg gcgtgatcag tggatagtcc gagccgaaca acatccggtc
    19741 cttgagctgc gaattggcgt atttcacgag ctgcggcgga aaatacttcg gcgaccagcc
    19801 cgacaggtcg atgtagacgt tcggcttgtg caggcacacc gacagcgcct cgtcctgcca
    19861 gggccatgac ggatgcgcga tgataaccgt catgtcggga aaatccgccg cgacgtcatc
    19921 gagatggatg gggttggaat acttcaggcg cagcccgccg ccgcccggca tgccggtgcc
    19981 catacccgag tgcccgctgt ggaaaatcgc cggaagcttg tgctccgcga tcaactcgta
    20041 gagtggatag gccatgcggt cgttggggaa aaagccctgc agagtcgggt gaaacttgaa
    20101 tcccttgatc acgccgtcct cgagcaggcg ccgcgcctcg cgcacgccca tcttgccctt
    20161 gtgcggatcg atgctggcga acgccatcat gatgtcggaa ttttcgcgtg ccgcgtccgc
    20221 gacttcctcg ttgggaatgc ggcgcgctcc gatctcgaat tccgagtcca ccgtgaacat
    20281 cacgagcccg atcttgcgct cgcggtagta cgcgatcgtc tcggggatcg tcggccgctt
    20341 gccggccttg aagtatttcg cggaggcatc ctcgaatttc ttccactctt cgtcgggctc
    20401 gtggcggcac gagacctcgg catgggtgtg aacatcgatg gcgacgaggt cctggatgtt
    20461 catgactccc actccgcaat cgtcatcttc ttctcgacga gttccacgag cagcggcgcc
    20521 ggctcccagt gcatggggcc gaaaatatcg cgatagcggc gcatgccctt cagcaaggtg
    20581 tcgaggccga tcacctcggc gtgaaacatg gggccgccgc gataccgcgg aaaaccatat
    20641 ccggaggtcc acaccacgtc gatgtcggcc gcgcgcatga cgatgccttc gcccaggatg
    20701 cggaagcctt cgttcatcag cgggaaaagg cagcgctcct ggatttcttc agtgctgtgc
    20761 gcgcgcgcgg gaacgttcag ctcccgtgat ttgcggcgca ggatttcgat ggcctccggg
    20821 tcgtcgagtc gcgcacgctt gcccgcctcg taacggtaat accccttgcc atttttctgg
    20881 cccaggcggc ccgcgtcgtg taacgccacg tccgcctggt agtaggccgg atccggagga
    20941 tattgcgagg cattggcccg atgaacgttg acgcccacgt cgatgcccgc catgtcgaac
    21001 accgcgagaa tgcccatggc catgccccag tcttcgagcg cgctatccac ctggcgtgga
    21061 gttgcaccct cgagcacgat gcgctgcgcc tcgcgcgcat acccttccat catgcggttg
    21121 ccaacgaacc cgtagcagac cttggccaga accggcgtct tgcgcagtgg cttggcgagt
    21181 tccatgaccg tcttgatcgc gactggcgag gtgtgcgtgg tccgcaccac ctcgagcagc
    21241 ggcatgacgt tggcgggcga gaaaaaatgc atgccaatca catccgcggg ccgtgacgtg
    21301 gcattcgcga tggtctcgat atccagcgtg gacgtgttcg tggccagcac tgccccgcgt
    21361 cgggcgaccg cgtcgagctt cgtgaacact tcacgtttga gatccatgct ctcgaacacg
    21421 gcctcgatga tgacgtcggc ttcgccaagg tcgccgtagt cgagtgtggt tcgtatgagc
    21481 ttctcgcgct gcccagcttc cgcagcggta agacggcccc gctccaccat ggactgatag
    21541 gtgcgcgtga cattggcgag gccgcgctcg agcgcggcgg cgttcgcatc gagcagcgtg
    21601 accggaatgc cggcgttggc gaagcagatc gcgatgccgc cgcccatggt gccggcgccg
    21661 atgaccgctg ctgacttgat aggtgccgca cgaatgtcgt cacccagccc cggtacccgc
    21721 cggctttcgc gctcggcgaa gaagacgtga atcgcgcctc gggattcgac gctggtcttg
    21781 cgctcgttga cgagcgccgt ttcgtattcg agtccttcgg acaggctgga atgcgccgcc
    21841 ttgcgcacgg cttccacggc taccaggccc gcgctccgat tgggataaag cttgcgcgcc
    21901 tgatccgaca gccgctggaa catttcatcc gacgcagtcg cagggtcgac gcgcatctcg
    21961 ccggtacgac gcggaccctt gccggcgctc agcagcgaac gcgcgtagtc gcaagcgccg
    22021 gtcttcagat cgccttcgat ggtctgatcg aggaacccca actccatggc ctgcggtgcg
    22081 ccgacgggct tggcggacag gatgaaatcc agtgcgcgct ccgcaccgat gagacgcggc
    22141 agacgttgcg tgccgcccgc gcccggaatg atgccgagcg tcacttcggg caaggcaaat
    22201 cgtgtcgtcg gcgcggcaat gcggtagtga ctcgccagcg caatctcgag tcccccaccc
    22261 atcacggtgc cgtgcatcgc ggcgatcacc ggaacttcga gtgcttccat ggcgttgaac
    22321 agggtgcggt attcgacttc gcgcggcgga ccactgaact ccccgatgtc ggcacccgaa
    22381 aagaacgtgc tgccctcaca catcaggatg atcgcgcgga tgtcgcgagc ggaccggatg
    22441 gcgtcgagga cttcacgcaa gccggcgcga acctccgccg tgatggtatt gaccggaggg
    22501 ttgtgagcga cgaccagcgc cagctcaccg ttacgctcga ggcgtacaca ttgcgaggcg
    22561 ctcaaggtca aaccggattc tggaatttct tgagcgcgtc gatcagcgcg gcgagctcgg
    22621 acgccgtgaa catggcccgg aacttctctt cgtgttcggc gattcgcgtc ttgcactttc
    22681 gcaaagtgct ttgccctgat ggcgtcaggt aaagctcctg gcgtcgccgg tcgaccgtcg
    22741 aacgtttgcg aatgacgaag ccgcgatctt ccaggcgatc cgtcagagcc atcatggtgg
    22801 cgcgatccat gcccaacgcc gcggccacct cgacctgcga aacgccaccg ttggcctgga
    22861 tcagccacag cacggccgcc tgcttctggg tgagtccgaa atcggacaca gcagccatga
    22921 agtcccgatg catggcgccc tgggcgcggc gaagcgtata gccgagcagg ctatccagct
    22981 cggcgaacat caattcgggc acttcgaatt ccgaaggcag ctctttgcgt ttcaggttgg
    23041 attcccgcgg catctggtct ctgggggatg agtggacagg cgcgaatcta ccgcgcttgc
    23101 caagtcgctc gcaatacaaa tagtatgtgt tacgtacaat aattccgaac ccgggggggg
    23161 ataaatggaa atgtttggcg ctaccagccg cagcatcacg aagcggcaca agatcgcggg
    23221 ctgtctcacg cttgcgatgc ctgtactcct tttgaacgcc ggcccagccg ccgcgcagac
    23281 tccacccgac accaccggcg cggagccaga ggcggaaggt ctcgaagcca tcgtcgtgac
    23341 agcacgacgt cgcgaagaaa gcctgcagga cacacccgtc gcggtcacgg cgttgagtgc
    23401 cgatgcgctc gaacgacagc agatcgtcag taccaccgac ctcgacaagg tggctcccaa
    23461 catccagttc cactcctacg gaaccctgac cggcaacaac tccgccgcgc aggtgttcat
    23521 ccgcggcatt ggacagaccg acgcgacgcc cgccgtcgac cccggcg
//
LOCUS       DASQJS010000403         9464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1515157, whole genome shotgun sequence.
ACCESSION   DASQJS010000403 DASQJS010000000
VERSION     DASQJS010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9464
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515157"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VEW08_15865"
     CDS             complement(<1..251)
                     /locus_tag="VEW08_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42270.1"
                     /translation="MSCAAQWMAAVAGLGLAFAGGAARAADFDLTPAETQRVENDEIV
                     IRATLDAIERRGSVRAAMLIDAPPAVVFESMTRCADAVR"
     gene            366..707
                     /locus_tag="VEW08_15870"
     CDS             366..707
                     /locus_tag="VEW08_15870"
                     /inference="COORDINATES: protein motif:HMM:NF022459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HYJ42271.1"
                     /translation="MKRALAVIVVGLLASSTAFAEGFRCGNKLVTEGASRSEVAAKCG
                     EPTDVTTARSVFRRPVVWGYGRPYFVGEDFIEIPVEIWLYNLGPNKLMRQIRFEGGYV
                     VDIDTLGYGYN"
     gene            complement(830..2611)
                     /locus_tag="VEW08_15875"
     CDS             complement(830..2611)
                     /locus_tag="VEW08_15875"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HYJ42272.1"
                     /translation="MKFPALLAAALLGACAASPPPQAALPEELVNKLVEEYFDKQLEL
                     SPMSATSIGDSRYDDRLDETTSAGFRERQIAVDQAFLERARRIDPAGLPPTARITYDI
                     FVSERELALEGRKYPEELMPLNQMTGLPMDLAVYGSGTGPQPFVTAKDYDRFLARLRQ
                     FPRWADGAIDMMRAGMARGVTLPRPAMAKVVPQLRGIVTANLADNIFWAPVAAMPKEI
                     SPKDRERISTAYAAALTNEVLPAYTRLADFIERNYLPAARTTVGWADLPDGQAWYRWR
                     IRGATTMDMPADEIHRLGLAEVARIRGEMLTVKNQVGFTGDLDAFFKFLEEDPQFYFS
                     SEDELLDAYRGVKRRIDALLPKLFADFPKADYEIRPVEAFREESAAGASYQAPSPDGK
                     RPGIFYINTHNLKAQPRFGLETLSLHEAAPGHHFQIAIQQELTDLPRIRRFNGYVSYA
                     EGWALYAESLGKELGVFTDPYQWYGRLSDEMLRAMRLVVDTGLHSKGWTREQAIQYML
                     DNSSLAESDVTAEVERYIVWPGQALGYKLGQLHISALRARAQAQLGASFDVRAFHSQI
                     LRDGAVPMDVLTAKIDRWTASKQRPVK"
     gene            2767..3447
                     /locus_tag="VEW08_15880"
     CDS             2767..3447
                     /locus_tag="VEW08_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ42273.1"
                     /translation="MSSAILIADDHPLFREALKLAITQAFAGARTFEADTVDSLLATL
                     HAHPEIDLLLLDLGMPGAHGFSGLVQVRAHYPSVPVVVISGREDQDIAQRTLTHGASA
                     FIAKSAAVPTIIDALQTILQGGVWSSPDLVPVAGGRGVSVPADEAAAARRVASLTPQQ
                     FRVLSMLCSGQSNKRIALQLQVSEATVKAHMTAILEKMGAENRTQAVLVAQRLALHQF
                     APPVEEEP"
     gene            complement(3414..6944)
                     /locus_tag="VEW08_15885"
     CDS             complement(3414..6944)
                     /locus_tag="VEW08_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HYJ42274.1"
                     /translation="MTTTVSGWVLMFASLAYMAGLFAIAWWGDRTKLYPDNPRWRPYI
                     YAFALGVYGSSWTFYGAAGTAKQDGLLYIAGYFGPLVLLAFGIRFYERLVRLAKNQNA
                     TSISDFLSARFGRSRRVGVLVTVIALTAIIPYIALQLTAISMSIDVLTGKPPGITPHW
                     YSDTTLGVALMLALFAILFGTRGIDATDHHPGMVLAIAAESVFKLLALVVVSVFALTT
                     LDSPAPVLTALRHIPEQFTQPSMFFAQALTAMFAFFCLPRQFHIGVVECADIGDIRRA
                     RWWFGGYLVLLSIVVIPIVAASLPLDAAGRSSPSTDALVLWIPLAAGQEWLALFAYLG
                     GYAAATGMVIVASVALATMISNDLVLPMLWRRRLTAVGESVAPPALILWVRRVAIVGV
                     LLGAYAFFHFTPNAPTLASIGTLSLAAVAQFAPAIIAAVYWPNASRAGVTGGLAAGFF
                     IWMYTLLVPSIVADGGSPRWVIYGPFGIEWLMPHRIFGFEFPENFSHGVFWSLTANVA
                     VMVALSRRYRPAIGERLREAGVRLLEGLPGTRGGQLLPGGATVGDLLVLAERLLGAPA
                     ARRLLERRSREIKRHVFAEERADLALLQSLERDLSGALGATSARLVLTSALRGAGIEL
                     AEIVTLFDEASQKLRFNRELLETMMDNMPQGVCVVNAEMRLVAWNQRYLDLFEYPPGF
                     VFAGKPVEELLRFNASRGWCGPGDLETQVRKRLAFMRAGSVHTSERRRTDGRVIELRG
                     QPLPDGGFVTTFSDVTSHKRQEESLREINETLELRVEERTRQLAAAVAQAEQANQSKT
                     RFVVAASHDLLQPLGAARLFNAALRSRAGQDGDLRGLAESVDNSLAAAGELLEGLLDI
                     SRLDAGGVRAEITEFDVGDLLNSLLKQFAPVAVSRGIRLSIIPTRLRVRSDPRLLRRV
                     LQNFIGNALRYTQSGSVVLGARRRAGATVELQVIDTGPGITPENRSAIFEEFRRLEQP
                     SPWGEKGLGLGLSICERIAAILDSTLTLESSPGRGSVFGICVPRAGAGAATSFSEFTE
                     HKIGRADDAISSVRILCVDDDAATLDGLRELLARWGFRVLAVASPEAALSLDVNYDIV
                     LADYHLQNRPAGLELLERLVHQAPGGQKRAGALLTADATEKLVQEADKLGFPVLRKPV
                     RPAALRALIAALVDRRARANHGSSSTGGAN"
     gene            7000..8298
                     /locus_tag="VEW08_15890"
     CDS             7000..8298
                     /locus_tag="VEW08_15890"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HYJ42275.1"
                     /translation="MLVGSQVRRVAVVGGMRIPFARGHGAYSRVGNQDMYTAVLQALV
                     AKYRLAGERLGDVTGGAVLKHSKDFNLVRECVLSSGLDPQTPGIDMQRACGTGLDAAI
                     SVAMKIALGQMDSGIAGGFDTVSDPPIVYSREYQQLLLRAFRGRSAWQRIKPFFGLRP
                     RHFKPVMPGVLEPRTGLSMGEHCEQMAKTWKISRADQDALANLSHVNAGKAWARGFFS
                     DLVVNDYQGLKIDNNVRSDSTVEKLGALKPAFDRRSGQGTLTAGNSTPLTDGASAVLL
                     ASEEWARQRNLPVLAYFSFSKQWAVDYVGGNEGLLMAPAYAVPAMLKDANLTLQDFDF
                     YEIHEAFAAQVLCTLKAWESPEYCKEKLGLDKPLGSIDREKLNVNGSSVAIGHPFAAT
                     GTRIVASLAKQLAESPTAKRGLISVCTAGGMGVVAILEKA"
     gene            8503..>9464
                     /locus_tag="VEW08_15895"
     CDS             8503..>9464
                     /locus_tag="VEW08_15895"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HYJ42276.1"
                     /translation="MKRYVHFPKVEGQASRQAHADLPAGTYEREISKEGFFGPAAHLY
                     HRHPPTGWVDFEGPLRPHAFDCTKLSDNQESPWNAVELLHNASVRVRFWSLAGSMPQL
                     ARNADGDELLFVHAGSGDLFCDYGHLAIEAGDYVVIPRGTMWRVECTQPLKALLIEAT
                     NNSYRLPDKGLVGPHAIFDAAMLDTPRIDEAFKDQQEDIEWRVAIKRRGAVSTVTFPF
                     NPLDAVGWHGDLSVVRINVRDLRPLMSHRYHLPPSAHTTFLADRFVVCTFVPRPFESD
                     PGAIKVPFFHNNDDYDEVLFYHAGDFFSRDNIHPGMITLHPGGFT"
BASE COUNT         1730 a         3098 c         3019 g         1617 t
ORIGIN      
        1 taccgcacgg catccgcaca gcgggtcatg gactcgaaaa ccactgctgg cggggcgtcg
       61 atgagcatgg cggcccgcac gctgccgcgg cgctcaatcg catcgagggt ggcgcggatc
      121 acgatctcgt cgttttcgac gcgctgcgtc tcggcggggg tgagatcgaa atccgccgcc
      181 cgcgcggcgc ccccggcaaa cgccagcccg aggcccgcaa cagccgccat ccattgcgct
      241 gcacagctca tggttaacga aatcccatcc ttaatgagtg ccgctcgcgt gccaaatggt
      301 tctaccggat cgaaacgccg atgtgttcat tgtccgttaa gcaggatgag cctatcgttt
      361 gtcccatgaa acgggcactt gccgtaatcg ttgtcggact gctggcctct tcgaccgcct
      421 tcgccgaagg gttccgctgc ggcaacaagc tcgtgaccga aggcgcgtcg cgatccgaag
      481 tggccgccaa gtgcggcgag cccaccgacg tgaccaccgc gcgcagcgtg ttccggcggc
      541 ccgtggtctg gggctatggg cggccgtact tcgtcggcga ggacttcatc gagatacccg
      601 tcgagatctg gctgtacaac ctcggcccta acaaactcat gcggcagatc cgcttcgaag
      661 gcggctacgt cgtcgacata gatacgctgg gctacggcta caactgagaa ggatggggtc
      721 aggcaccgat tccccgcgaa gcggacaaaa ggggtcagac ttctcttcga agtctgaccc
      781 cttttgttag gaatcggtgc ctgaccccat tcttatcggc accactcgtt tatttgacgg
      841 gtctctgctt cgacgcagtc catcggtcga tcttcgcggt cagcacgtcc atgggaaccg
      901 cgccatcgcg caggatctgg ctgtgaaatg cgcgcacgtc gaaggacgca ccgagctgtg
      961 cctgcgcgcg ggcacgcagc gcgctgatgt gcagctggcc taacttgtag cccagcgcct
     1021 ggccgggcca cacgatgtac cgttccacct ccgcggtgac gtcgctctcg gcgagcgacg
     1081 aattgtcgag catgtactgg atggcctgtt cgcgggtcca ccccttcgaa tgcagccccg
     1141 tgtccaccac caggcgcatg gccctcagca tttcgtcgga caaccttccg taccactggt
     1201 acgggtccgt gaagacgccg agttccttgc cgagggattc cgcgtacaac gcccaacctt
     1261 ccgcatagga cacgtagcca ttgaagcgcc gaatgcgtgg aagatcggtg agctcctgct
     1321 ggatggcgat ctggaagtgg tgccccgggg ccgcttcgtg cagcgagagc gtctcgaggc
     1381 cgaagcgcgg ctgcgctttg agattgtgcg tgttgatgta aaaaatgccc gggcgcttgc
     1441 catcgggcga aggcgcctgg tacgaggcgc ccgcagcgga ctcctcccgg aatgcttcga
     1501 ccggccggat ttcgtagtcc gccttcggaa aatccgcaaa caacttcggc agcagcgcgt
     1561 cgattcgccg cttgacgccg cgataggcat ccagaagctc gtcctcgctc gagaagtaga
     1621 actgcgggtc ttcctccagg aatttgaaga atgcgtcgag atcacccgtg aagccgacct
     1681 ggttcttcac cgtgagcatt tcgccccgga tgcgcgcgac ttccgccagg cccagacggt
     1741 gtatttcgtc ggccggcatg tccatggtgg tggcgccgcg aatgcgccag cgataccacg
     1801 cctggccgtc gggcaggtcc gcccaaccga ccgtggtacg cgccgccggt agatagttac
     1861 gctcgatgaa atccgcgagc cgcgtatacg cgggcaggac ttcattggtg agcgcggccg
     1921 cgtaggcggt actgatgcgc tcacgatctt tcggggagat ttcctttggc atcgccgcga
     1981 ccggcgccca gaagatgttg tcggccaggt ttgccgtgac gatgccgcgc agctgcggaa
     2041 ccaccttggc catggccggg cgcggcaacg tgacgccgcg cgccattccc gcgcgcatca
     2101 tgtcgatggc gccatcggcc cagcgcggga actggcgaag gcgcgccagg aaccggtcgt
     2161 agtcttttgc cgtgacgaat ggctgcggac cggtgcccga gccatacacc gccagatcca
     2221 tgggcaggcc ggtcatctgg ttgagcggca tcagctcctc gggatatttg cgaccttcga
     2281 gcgcgagttc gcgctcgctc acgaatatgt cgtaggtgat gcgtgccgtt ggcggcagcc
     2341 ccgcgggatc gatgcggcgt gcgcgttcga gaaaagcctg gtccacggca atctggcgtt
     2401 cgcgaaagcc cgcactggtg gtttcgtcca gccggtcgtc gtagcgcgaa tccccgatgg
     2461 acgtggcgga catgggtgac agttcgagct gcttgtcgaa gtattcctcg acgagcttat
     2521 tgaccagttc ttccgggagc gcggcttggg gcggcggcga ggcggcgcat gcgccgagca
     2581 acgcggcagc taacagggca ggaaatttca tgcgaggaat ggtagcaggc gaccgccgga
     2641 cgccacggcg gcaagccccc gcgttcgcgc cgaaatgggc gttcatcgcg ccagcctgcg
     2701 aattaatgtc attggcacgg gtcctaggtc cggcacgcac aaagcattaa cctacggccc
     2761 acaactatgt catccgccat cctcattgcc gacgatcacc cgctgtttcg cgaagcgctc
     2821 aagctcgcca tcacccaggc tttcgccggt gctcgcacct tcgaagccga caccgtcgac
     2881 tccctgctcg cgacgctgca tgcacacccg gaaatcgacc tgctgctgct cgatctcggc
     2941 atgccgggcg cgcatgggtt ttccggcctg gtgcaggttc gcgcgcatta cccgtcggtg
     3001 cccgtggtcg tcatttccgg ccgcgaggac caggacatcg cgcaacggac gcttacgcat
     3061 ggtgcgtcgg ccttcatcgc caaatccgcg gccgtgccca ccatcatcga cgcgctgcag
     3121 accatactgc agggcggtgt ctggagctca ccggacctgg tgccggttgc aggcggacgc
     3181 ggcgtcagcg tacctgcgga cgaagccgcg gccgcgcgtc gcgtggccag cctcacgccg
     3241 cagcagttcc gcgtgttgtc catgttgtgt tccgggcaat cgaacaagcg tatcgcgctg
     3301 cagttgcagg tctccgaagc aaccgtgaag gcgcacatga cggccatcct cgaaaagatg
     3361 ggcgccgaaa accgcacgca ggcggtgttg gtcgcgcagc gcctcgcgct gcatcagttc
     3421 gcgccgcccg tcgaagagga accgtgattc gcgcgcgccc gccggtcgac gagcgcggcg
     3481 atcaacgcac gcagcgcggc gggccgcacg ggcttgcgca acaccggaaa tccgagcttg
     3541 tcggcctcct gcaccagctt ctcggtggca tcggcggtca gcagcgcgcc cgcgcgtttc
     3601 tgtccgccgg gcgcctggtg caccaggcgc tctaacaact cgagccccgc ggggcgattc
     3661 tgcaggtgat agtcggcgag cacgatgtcg tagttgacgt cgagcgacag cgcggcttcg
     3721 ggtgaagcga cggccagcac gcggaatccc catcgcgcca gcaattcgcg caatccgtcg
     3781 agcgtggcgg catcgtcatc gacgcacagg attcgcacac tggagatggc gtcatcggcc
     3841 cggccgatct tgtgttcggt gaactcgctg aagctcgtcg cggcgcccgc accggcgcgt
     3901 ggtacacaaa tgccgaagac gctgccgcgc ccgggcgacg attcgagcgt gagtgtgctg
     3961 tcgaggatcg cggcgatgcg ttcgcagatc gacaggccca gtcccaaacc cttttcgccc
     4021 cagggcgaag gctgttcgag cctgcggaat tcttcgaaaa tggcgctgcg attctcggga
     4081 gtgattcccg gccccgtgtc gatgacctgc agctcgacag tggcgccggc gcggcgccgg
     4141 gccccgagca ccacgctccc ggactgcgtg tagcgcaacg cgttgccgat gaagttctgc
     4201 aggacgcgcc tgagcagacg tggatcggaa cgcacgcgca gccgcgtggg aatgatcgag
     4261 agccggatac cgcgcgatac cgcaaccggc gcgaattgct tcagcagtga gttcagcagg
     4321 tcgccgacgt cgaattccgt gatctcggcg cgtacgccac ccgcgtcgag gcgcgagata
     4381 tcgagcagtc cttccagcaa ctcgccggcc gcggccagcg aattgtcgac gctctcggca
     4441 agcccgcgca gatccccgtc ctggccggcg cggcttcgca gcgcggcgtt gaagagccgc
     4501 gccgcgccga gcggctgcaa caggtcgtgg ctcgcggcaa cgacgaaccg cgtcttcgat
     4561 tggtttgcct gctccgcctg cgcgaccgcg gccgcgagct gacgcgtgcg ttcttcgact
     4621 cgcaactcga gcgtttcgtt gatttcacgc aggctttctt cctggcgttt gtggctggtg
     4681 acgtcgctga acgtagtgac gaagccgcca tcgggcagcg gctggccgcg cagttcgatc
     4741 acgcgcccat cggtgcggcg tcgctccgag gtgtgcacgg agccggcgcg catgaacgcg
     4801 agacgcttgc gcacctgagt ttcgaggtcg ccaggcccgc accatccgcg gctggcgttg
     4861 aagcggagca gctcttccac gggcttgccg gcgaatacga accccggtgg gtactcgaac
     4921 aggtccaggt agcgctgatt ccaggcgacg agccgcatct ccgcgttcac tacgcacaca
     4981 ccctgcggca tgttgtccat catcgtttcg agcagctcgc gattgaaacg cagcttctgc
     5041 gacgcctcgt cgaacaacgt gacgatttcc gcgagctcga tgcccgcgcc gcgcaacgcg
     5101 ctggtcagca ccaggcgtgc cgatgtcgcg cccagcgcgc cggagagatc acgctcgaga
     5161 gactgcagca acgcaaggtc cgcgcgttcc tcggcgaaca cgtggcgctt gatctcacgc
     5221 gagcgccgct ccagcagccg ccgtgccgcc ggtgcgccta acaatctttc cgcgagcacc
     5281 agcaggtcgc cgaccgtggc gccacccggc agcaactggc ctccgcgcgt gccgggcaag
     5341 ccttcgagca ggcgcacgcc cgcctcgcgc agccgttcgc cgatcgccgg gcgatagcgg
     5401 cgcgacagcg cgaccatgac cgcgacgttc gcggtgaggg accagaacac gccgtgggag
     5461 aagttttcgg ggaactcgaa gccgaaaatc ctgtgcggca tcagccactc gattccgaag
     5521 ggcccgtaaa tcacccagcg cggcgagccg ccgtcggcca cgatgctggg aaccagcaac
     5581 gtgtacatcc agatgaagaa gccggccgcg agaccaccag tgacgccggc gcggctcgca
     5641 ttcggccagt acaccgcagc gatgatggct ggcgcgaact gcgctaccgc ggccagcgac
     5701 agggtgccga tggacgccag ggtcggcgcg ttgggggtaa agtgaaagaa cgcgtacgcg
     5761 cctaacaaga cgcccacgat ggccacgcgg cgcacccaca ggatcaacgc cggtggtgcc
     5821 acgctttcgc ccacggccgt caaccgccgg cgccacaaca tcggcagcac caggtcgttg
     5881 ctgatcatgg tcgccaacgc gacgctcgcg acgatgacca tgcccgtggc cgcggcgtaa
     5941 ccaccgagat acgcgaacag cgccagccac tcctgcccgg ccgcgagtgg aatccacaac
     6001 accagcgcat cggtgctcgg cgagcttcga cccgcggcat cgagtggcaa ggatgccgcg
     6061 acgatgggta tgaccacgat gctgagcagc acgaggtatc cgccgaacca ccaccgcgca
     6121 cgcctgatgt ccccgatatc cgcgcactcg acgacgccga tgtgaaactg ccgtggcagg
     6181 cagaagaacg caaacatggc ggtcaacgcc tgggcgaaga acatcgacgg ctgcgtgaac
     6241 tgttcgggta tgtggcgcag cgcggtgagc acaggggccg gactatccag tgtcgtcagc
     6301 gcgaacacgc tcacgaccac cagcgccagc agcttgaata cggattcggc ggcgatggcc
     6361 agcaccatgc cgggatgatg atcggtcgcg tcgatgccgc gcgtgccgaa cagtatcgcg
     6421 aacagcgcga gcatgagcgc gacacccagc gtggtgtccg aataccaatg cggtgtgatg
     6481 cccggcggct tgccggtgag cacgtcgatg ctcatggaga tggcggtgag ttgcagcgcg
     6541 atgtacggga tgatcgcggt gagcgcgatg accgtgacca gcaccccgac gcggcgcgaa
     6601 cgtccgaagc gcgctgacag gaagtcggag atcgacgtgg cattctgatt cttggcgagc
     6661 cgcaccagcc gctcgtagaa ccggatgccg aatgcgagca ataccagcgg tccgaaatag
     6721 ccggcgatgt acagcaggcc gtcctgtttc gccgtgcccg cggcgccata gaaggtccag
     6781 ctcgatccgt agacacccag ggcgaacgcg tagatgtaag gccgccaccg ggggttgtcg
     6841 ggatagagct tggtgcgatc gccccaccac gcgatggcga acaggccggc catgtacgcc
     6901 agcgacgcaa acatcaatac ccagcctgaa acagtcgtcg tcatcggtgg ccagacgata
     6961 gttgaattct ggcatcctag cgtcaaagag gtgttggcca tgcttgtggg atcccaagtt
     7021 cgtcgcgtcg ccgtggtcgg cggcatgcgc attccttttg cgcgtggcca cggcgcctat
     7081 tcgcgcgtcg gcaaccagga catgtacacc gcggtgctgc aggcactggt tgccaaatac
     7141 cgcctcgcgg gcgagcgcct gggagatgta accggcggcg cggtgctcaa acactccaag
     7201 gacttcaacc tggtgcgcga gtgcgtgctg tcctccggcc tcgatccgca gactcccggc
     7261 atcgacatgc agcgcgcgtg cggcaccgga ctcgacgccg cgatttccgt ggccatgaaa
     7321 attgcgctgg ggcagatgga ctccggcatc gccggtgggt tcgacacggt cagcgatccg
     7381 cccatcgtct attcgcgcga gtaccagcag ctgttgttgc gcgcgtttcg cggacgcagc
     7441 gcatggcaac gcatcaagcc cttcttcggt ttgcgcccgc ggcacttcaa gccggtcatg
     7501 cccggcgtgc tcgaaccgcg caccgggctt tcgatgggtg agcactgcga acagatggcc
     7561 aagacctgga agatctctcg cgccgaccag gatgcactcg cgaatctcag ccacgtgaac
     7621 gccggcaagg cgtgggcgcg gggcttcttc agcgatctgg tggtgaacga ctaccagggt
     7681 ctcaagatcg acaacaacgt gcgaagcgac agcacggtgg aaaagctcgg cgcgctcaag
     7741 ccggcattcg atcgccgcag cggccagggc acgctcaccg caggaaattc cacgccgctc
     7801 accgacggcg cttcggccgt gttgttggcc agcgaagaat gggcgcggca acgcaatctg
     7861 ccggtgctgg cgtatttcag cttcagcaag cagtgggcgg tcgactacgt cggcgggaac
     7921 gaaggcctgc tgatggcgcc tgcctacgcg gtgccggcca tgctcaagga tgcgaacctc
     7981 acgctgcagg atttcgactt ctacgaaatt cacgaagcgt tcgcggcgca ggtgctgtgc
     8041 acgctgaaag cctgggaatc gcccgagtac tgcaaggaga aactcgggct ggacaagccg
     8101 ctcggcagca tcgaccgcga gaagctcaac gtgaacggct cgagcgtggc catcggacat
     8161 ccgtttgccg ccaccggcac gcgcatcgtc gcgtcgttgg ccaagcagct ggccgagtcg
     8221 ccgaccgcga agcgcggtct tatctccgtt tgcaccgccg gcggaatggg cgtggtggcg
     8281 attctcgaga aagcttgagc acggtgccgg gtggggagtg gggagatgac tccccactcc
     8341 ccacccggca ccagcctcct cttccaattg gtttcaaatg aaactaaatt gatggtattg
     8401 gggacattcc tcatttccgc cgtaacaatt ggctgaattc cccaagcggt cggtggcgta
     8461 cacaattgtt acggcggaaa tgaggaatgt ccccaatacc atatgaagcg ctacgtccac
     8521 tttccgaagg ttgagggcca ggcttcgcgc caggcgcacg ccgacctgcc cgcgggcacg
     8581 tacgagcgag agatcagcaa ggaaggcttc ttcggtcccg cggcgcatct ctatcaccgc
     8641 catccgccca cgggctgggt cgacttcgaa ggcccactgc gaccgcacgc tttcgactgc
     8701 acgaagctga gcgacaacca ggagtcaccc tggaacgccg tggagctgct gcacaacgct
     8761 tcggtgcgcg tgcgcttctg gagtctcgcc ggcagcatgc cgcagctcgc gcgaaacgcc
     8821 gatggcgacg aactcctgtt cgttcacgcc ggaagcggcg acctgttctg cgactacgga
     8881 catctggcca tcgaagccgg cgactacgtc gtcattcctc gcggcacgat gtggcgcgtg
     8941 gaatgcaccc agccgctcaa ggcgctgctc atcgaagcca ccaataattc ctaccggctg
     9001 cccgacaagg gcctggtcgg accgcacgcc atcttcgacg cggccatgct cgatacgccg
     9061 cgcatcgacg aggccttcaa ggatcagcag gaagacatcg agtggcgcgt ggccatcaag
     9121 cgccgcggcg ccgtctccac cgtcaccttc ccgttcaatc cgctggacgc cgtgggctgg
     9181 catggcgatc tgtcggtggt ccgcataaac gtgcgggatc tgcggccact catgagccac
     9241 cgctatcacc tgccgccgtc ggcgcacacc acgttcctcg ccgaccgctt cgtcgtatgc
     9301 acattcgtgc cgcgcccgtt cgaatccgat ccgggcgcca tcaaggtgcc cttcttccac
     9361 aacaacgacg attacgacga ggtgttgttc tatcacgccg gcgatttttt cagccgcgac
     9421 aacattcacc ccggcatgat cacgctgcac ccgggcggct tcac
//
LOCUS       DASQJS010000404         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3089293, whole genome shotgun sequence.
ACCESSION   DASQJS010000404 DASQJS010000000
VERSION     DASQJS010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3089293"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VEW08_15900"
     CDS             <1..795
                     /locus_tag="VEW08_15900"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HYJ42277.1"
                     /translation="GREAAGLVSAVGKGVRGFRVGDRVAYVMSPPGSYADLRNVPAAR
                     VVKIPAGVSDEHAAVLALKGMTACFLLRHCFRVERGDVIVVHAAAGGVGSLLSQWGRK
                     LGAEVVGIVGSEDKVKLAKKNGCKHVLVRGRDEIAVSVRKFSKGLGAHVVYDGVGKDT
                     FVESLDSLRKRGMMVSYGNASGPVPPFSPAELVKRGSLFLTRPTMFDYTESREDLEAI
                     ARETFGAAKKGWIKVQIHQRYKLADAAQAHRDLEARKTTGASVILP"
     gene            complement(824..1324)
                     /gene="ilvN"
                     /locus_tag="VEW08_15905"
     CDS             complement(824..1324)
                     /gene="ilvN"
                     /locus_tag="VEW08_15905"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HYJ42278.1"
                     /translation="MRHIIAIHLQNEAGALTRVTGLFSTRGYNIESLSVAATDDPSVS
                     RVTLVTTGADNVILQIVNQLNKLIDVVAVEDLTRGEHIERELVVLKLRVEPQLVDSVR
                     GYVVRAGGRVLEPSPEGFVVELTASEVEVTQFVTDLGERTDIVEVVRSGALAVGRNAR
                     ALHVVR"
     gene            1452..>2020
                     /locus_tag="VEW08_15910"
     CDS             1452..>2020
                     /locus_tag="VEW08_15910"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ42279.1"
                     /translation="MTATGTPAAPTIAHPLAGQKMTGAEMIVQVLADEGVSTIFGYSG
                     GAILPTYDAVFCFNEDRAKKGEPQMPLIVPANEQGAGFMAAGYARASGRVGVAIVTSG
                     PGATNTVTPIRDCMADSIPIVVICGQVPTSAIGTDAFQEAPVPSIMGSVAKHIFLVTD
                     PAKLETTVRTAFEIARTGRPGPVVIDVPK"
BASE COUNT          370 a          633 c          679 g          338 t
ORIGIN      
        1 gggcgcgaag ccgcaggact cgtgtcggcg gttgggaagg gcgtgcgcgg atttcgcgtc
       61 ggtgatcgtg ttgcatacgt aatgtcgccg cccgggagct acgccgatct tcgcaacgtg
      121 cccgcggcgc gagtcgtgaa aattccggcc ggtgtgagcg acgaacatgc cgcggtgctt
      181 gcgctcaagg gcatgacagc ctgtttcctg ctgcgccact gctttcgcgt cgaacgcggc
      241 gacgtgatcg tcgtgcacgc ggcggccggc ggggtcggct cgctgctctc gcaatggggc
      301 aggaagttag gcgccgaggt cgtgggcatc gtcggcagcg aagacaaggt gaagctcgca
      361 aagaagaacg gttgcaagca cgtgctggtg cgcggccgcg atgaaatcgc ggtgagcgtc
      421 cgaaaattca gcaagggcct tggcgcacac gtggtgtatg acggcgtggg caaggacacg
      481 ttcgtcgagt cgctcgacag cctgcgcaag cgcggcatga tggtgtcgta cggcaacgct
      541 tcgggaccgg tgccgccgtt ctcgcccgcc gagctcgtga agcgtggctc gctgttcctg
      601 acgcggccga cgatgttcga ctacaccgag tcgcgcgagg atctcgaggc gatcgcccgc
      661 gagacgttcg gagcggcgaa gaagggctgg atcaaggtgc agatccacca gcgctacaaa
      721 ctggcggacg cggcgcaggc gcatcgcgat ctcgaagcgc gcaagaccac gggcgcgagc
      781 gtgatccttc cctagggtag gagcgcgctg cgccgggtgc cggttatcta actacgtgca
      841 acgcccgcgc attgcgcccg accgctagcg cgccggagcg aaccacttcg acgatgtcgg
      901 tgcgttcacc gaggtcggtc acgaactggg tgacctcgac ttcactcgcg gtcagctcca
      961 ccacgaagcc ttcgggcgag ggctcgagta cgcggccgcc ggcacgcacg acgtaaccgc
     1021 gaacggaatc gaccaactgc ggttcgacgc gaagcttgag caccacgagc tcgcgctcga
     1081 tgtgctcgcc acgcgtgaga tcttccaccg ccaccacgtc gatgagcttg ttgagctggt
     1141 tgacgatctg caggatcacg ttgtccgcgc ccgtggtcac cagcgtgacg cgtgataccg
     1201 aggggtcatc cgtggcagct accgacagcg actcgatgtt gtagccgcgg gtggaaaaca
     1261 gaccggtgac gcgggtaagc gcgccggcct cgttctgcag gtggatggcg atgatatgac
     1321 gcatggtttt cgggcaatcg ggtcggcgga aacggcggcc agaatagcgg tttgcgccca
     1381 atccgttcaa tcaaacaata caaaatgcta ggctcccgcg ggctatggca aaaaccccca
     1441 aaatccgtga cttgaccgcg actggcaccc cggctgcgcc caccatcgcc catccgcttg
     1501 caggtcagaa aatgaccggc gcggagatga tcgtgcaggt actcgcggac gagggggtat
     1561 cgaccatttt cggctacagc ggcggcgcca tcctgcctac ctacgacgcc gtcttctgct
     1621 tcaacgaaga ccgcgcgaag aagggcgagc cgcagatgcc gctcatcgtg ccggcgaacg
     1681 agcagggcgc gggtttcatg gccgcaggtt atgcgcgcgc cagcggccgc gtgggcgtgg
     1741 ccatcgtcac gtcgggtccc ggcgccacca acacggtcac cccgatccgc gattgcatgg
     1801 cggattccat tcccatcgtc gtcatctgcg ggcaggtgcc caccagcgcc attggaacgg
     1861 atgcgttcca ggaagcgccg gtgccttcca tcatgggctc ggtggccaag cacatttttc
     1921 tcgtcaccga tccggcgaaa ctcgagacca ccgtgcgcac cgcgttcgaa atcgcgcgca
     1981 ccggccgccc gggccccgtg gtcatcgacg tgccgaagga
//
LOCUS       DASQJS010000405         4838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3715779, whole genome shotgun sequence.
ACCESSION   DASQJS010000405 DASQJS010000000
VERSION     DASQJS010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3715779"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VEW08_15915"
     CDS             complement(<1..614)
                     /locus_tag="VEW08_15915"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42280.1"
                     /translation="MEFIRGIQGLKPHHRGAAITVGSFDGIHLGHGALIARACALAAH
                     AGKPAIMLTFEPLPREYLMSQAQPAQEPPARLTDLRERWRVLSHSDLNAVCVLRFNEK
                     LRSLSGEQFVNLLAEQFAASAVVVGQDFRFGRGGAGSVALLQAAADAGRFALELVPSV
                     CVDEVRVSSSGVRTALAAGDFHAARDLLGRPYSMRGRVVTGEKLG"
     gene            complement(620..2182)
                     /gene="murJ"
                     /locus_tag="VEW08_15920"
     CDS             complement(620..2182)
                     /gene="murJ"
                     /locus_tag="VEW08_15920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HYJ42281.1"
                     /translation="MSGAIFKSTAVTGVATLVSRITGLAREMIYAQAFGASAFMDAWL
                     VAFKIPNFLRRLFAEGSFSQAFVPVISEYKSKRSKDEARELVDSVAGTFGVLLLVVTL
                     LGVIAAPLIILAFAPSWHNEPDKWHLATSMLRWTFPYLFFISLSALFSGVLNSYGKFA
                     LPAYTQVIMNVVMIIVALWIAPHTDNPGVALAMGVFVSGLLQLGFQVPAVMRLGLFRW
                     PRWRPAADGVRRIGRLMLPGVFGSSVAQVSLLLDTQIATFLITGSVSWLYYADRLMEF
                     PLGVFSIALATVILPGLSRHHADESPEHFTATIDWALKLVILLVSPAAVAMLVFAGPL
                     TATTFGYGAFLPHDVKMASYALMAYSWGLLGFSLVKVLAPGYFARQDTRTPVRVGLIA
                     LGCNMAINIAVVLPAHYLGFPYPHILIATSTCISAAINTTLLWRGLVRAGVYKPRPGW
                     TVLLARVLFANAAMAALLIWLGGDISGWLAASPLERAARLAMCVVAAAATYFAMLFVS
                     GLRLRHMRNAAA"
     gene            2324..2587
                     /gene="rpsT"
                     /locus_tag="VEW08_15925"
     CDS             2324..2587
                     /gene="rpsT"
                     /locus_tag="VEW08_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HYJ42282.1"
                     /translation="MANTKSAEKAARRAETNRNRNVALRSRMRSALRKVVDAVAAGDK
                     AIASSALKEAQPVIDSLVNKNVIHRNKAARHKSALTARIKAMK"
     gene            complement(2690..3910)
                     /gene="obgE"
                     /locus_tag="VEW08_15930"
     CDS             complement(2690..3910)
                     /gene="obgE"
                     /locus_tag="VEW08_15930"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HYJ42283.1"
                     /translation="MKFVDEARVRVQAGNGGRGCISFRREKFVPFGGPDGGDGGLGGS
                     VTFRAREGINTLVDFRVERTFRAENGEPGGGRDMTGRGGKDTTVMVPVGTIVRDAETG
                     EVLGDMAKADMELLVARGGKGGWGNQRFKSSKNRSPRQFGPGLPGEQRALELELKVIA
                     DVGLLGLPNAGKSTLITAVSAARPKIADYPFTTLYPNLGVISCGNQRSFVMADIPGLI
                     EGAADGAGLGIRFLKHLQRTRVLLHLVDIAPTDPSADPVRDANSIVGELGKFSEDLLT
                     KPRWLVLNKRDLLPDDEAVKLAVSIAVALKYRGPAFLISGVTHAGTTDLTEAVMRFLE
                     TGEAPAQGNLELPVIAPKPTAKAKPVKAAKVAKKKVPKKKKAAPRKKAPARKKSPARV
                     KRPAKRPAKKRARR"
     gene            complement(4028..4285)
                     /gene="rpmA"
                     /locus_tag="VEW08_15935"
     CDS             complement(4028..4285)
                     /gene="rpmA"
                     /locus_tag="VEW08_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HYJ42284.1"
                     /translation="MAHKKAGGSTRNGRESHSKRLGVKKFGGETVLAGNILVRQRGTT
                     MRAGSNVGIGTDHTLFALKPGRVKFAKKGEEQHTFVSIDAE"
     gene            complement(4324..4635)
                     /gene="rplU"
                     /locus_tag="VEW08_15940"
     CDS             complement(4324..4635)
                     /gene="rplU"
                     /locus_tag="VEW08_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HYJ42285.1"
                     /translation="MYAVIATGGKQYRVEKGTVLKVENLAGDPGSTIEFGEVLLIGDG
                     DNIKVGAPLLKGGKVTATVEKHGKGEKKVIVKFRRRKHYLRQGTHRQQYTQVKITGIS
                     A"
BASE COUNT          926 a         1601 c         1475 g          836 t
ORIGIN      
        1 cccagttttt cgccagtgac gacgcgcccg cgcatcgaat agggtcggcc taacaagtcg
       61 cgcgccgcgt gaaaatcccc ggccgccagc gccgtgcgga caccgctcga actcacgcgg
      121 acttcgtcca cgcacaccga cggtaccagc tcgagcgcaa accggccggc atccgccgcg
      181 gcctgcagca gcgccacgct gccggcgccg ccacgcccga aacgaaagtc ctgcccgacc
      241 accaccgccg aggcggcgaa ttgctccgcc agcaggttca cgaactgctc gccggacagg
      301 cttcgcagct tctcgttgaa ccgcaacaca cacaccgcgt tgaggtccga atgcgagagc
      361 acgcgccagc gctcgcgcag gtccgtgagc cgcgccggcg gctcctgggc cggctgagcc
      421 tgggacatca ggtattcgcg cggcagcggc tcgaacgtga gcatgatcgc cggcttcccg
      481 gcgtgcgccg ccagcgcaca ggcccgcgca atcaacgcgc cgtgtccgag gtggatgcca
      541 tcgaagctgc ccaccgtgat ggcggctcct cgatgatggg gcttcaggcc ctgtattcca
      601 cgtatgaatt ccatggcgat caggccgcgg cgttgcgcat gtggcgcagg cgcaggccgc
      661 tcacgaacaa catggcgaaa taggtcgcgg ctgccgccac tacgcacatc gcgagccgcg
      721 cggcgcgttc cagcggcgat gcggccagcc agccggaaat gtcgccgccc agccagatga
      781 gcagcgcggc cattgccgcg ttggcgaaca ggactcgcgc cagcaacaca gtccaccccg
      841 gccggggctt gtagacaccg gcacgcacca gcccgcgcca gagcagggtc gtattgattg
      901 ccgcggagat gcaggtcgac gtggcgatca ggatgtgcgg atagggaaag ccgagatagt
      961 gggccggcaa caccacggca atgttgattg ccatgttgca gccgagcgcg atgaggccca
     1021 cgcgcaccgg agtccgcgtg tcctggcgcg cgaaatatcc cggcgccagc accttcacga
     1081 ggctgaagcc gagcaggccc catgaatagg ccatcaacgc gtagctggcc attttcacgt
     1141 cgtgaggcag gaacgcgccg tagccgaagg tggtggccgt gagcggacct gcaaacacca
     1201 gcatcgcgac cgcggccggt gaaaccagca ggatcacgag cttcagagcc cagtcgattg
     1261 tggcggtgaa atgctccggg gattcgtccg cgtgatgacg cgaaaggccc ggaaggatca
     1321 cggtggccaa cgcgatgctg aagaccccga gcgggaattc catgaggcgg tccgcgtagt
     1381 acagccacga cacgctgccg gtgatgagga acgtggcaat ctgtgtgtcg agcaacaggc
     1441 tgacctgcgc caccgacgaa ccgaatacgc ccggcaacat cagtcggccg atccgtcgca
     1501 caccatcggc ggcgggacgc caccgtggcc agcggaacag accgaggcgc atcacggcgg
     1561 gcacctggaa tcccagctgc aagaggccgc tgacgaacac ccccatggcc agcgccacgc
     1621 ccgggttgtc ggtgtggggc gcaatccaca gcgccacgat gatcatgacc acgttcatga
     1681 tcacctgcgt gtacgccggc aacgcgaact tgccgtagct gttgagcacg ccggagaaca
     1741 gcgcgctcag cgagatgaag aacaggtagg gaaacgtcca gcgaagcatc gacgttgcca
     1801 ggtgccactt gtcgggctcg ttgtgccagc tcggcgcgaa cgcgaggatg atgagtggcg
     1861 cggcgatgac gcctaacaac gtaacgacca gcaacagcac gccgaaggtg ccggcgacgg
     1921 aatccacgag ttcgcgcgcc tcgtctttcg agcgcttcga cttgtattcg ctgatgacgg
     1981 gcacgaaggc ctgcgaaaac gagccttcgg cgaacaggcg gcgcaggaag ttcgggatct
     2041 tgaacgccac gagccaggcg tccatgaagg cgctggcgcc gaacgcctgc gcgtaaatca
     2101 tctcgcgggc caatccggtg atacgcgaca ccaacgtcgc cacgccggtc acggcggtgc
     2161 tcttgaaaat cgctccactc accctgcaag gccccgaaaa ataaaggaaa aactgcgcaa
     2221 gccgcgcact ataccggact cggctttcaa gcgttgacaa ggtacaggtc ggtcagcaca
     2281 ctacgcggcc tcaaaacgac cctgatttgc tggagattcc ttcgtggcga ataccaagtc
     2341 ggccgaaaaa gccgcccgtc gagcagaaac caaccgcaat cgcaacgttg cgctgcgttc
     2401 acgcatgcgc tctgccctgc gcaaggtcgt cgacgccgtg gccgctggcg acaaggccat
     2461 tgcatcctct gcgctcaagg aagcccagcc ggtcatcgac tcgctggtca acaagaacgt
     2521 catccatcgc aacaaggccg cccgccacaa gagcgcgctc acggcccgca tcaaggcgat
     2581 gaagtaagag agaaggttaa gacttcggcg cctcacggcg cggagctatg aaagagccgg
     2641 cgttagccgg ctttttcgtt ttcggaaggt cgttcgtttc ggaatcgggc tatcggcgcg
     2701 cgcgtttctt cgcggggcgc tttgcgggtc gcttcacgcg ggccggagat ttcttcctgg
     2761 ccggcgcctt cttccggggc gccgccttct tctttttcgg caccttcttc ttggcgactt
     2821 ttgccgcctt cacgggcttg gccttcgccg tgggcttcgg cgcgatcacc ggcagttcca
     2881 ggttgccctg cgccggcgct tcgccggttt ccagaaagcg catgaccgct tcggtcaggt
     2941 cggtcgtccc ggcatgcgtg acaccagaaa tgagaaacgc cgggccgcga tacttcagcg
     3001 ccacggcaat cgaaaccgcc agcttcacgg cctcgtcatc gggcagcaga tcgcgcttgt
     3061 tgagcaccag ccatcgcggc ttggtcagca agtcttcact gaacttgccg agttcgccga
     3121 cgatggagtt cgcgtcgcgc accggatccg cggaaggatc cgtaggcgcg atgtccacca
     3181 ggtgcagcag cacgcgagtg cgctgcaggt gcttgagaaa gcgaatgcca aggcccgcgc
     3241 cgtcggccgc gccttcgatg aggcccggga tgtcggccat cacgaagctg cgctggttgc
     3301 cgcaggagat gactccgagg ttcggataca gcgtcgtgaa gggatagtcg gcgatcttcg
     3361 ggcgcgcggc cgacaccgcc gtgatgagcg tcgacttgcc ggcgttcggc aggcctaaca
     3421 aacccacatc ggcgatgact ttgagctcga gttcgagcgc acgctgctca cccggcagtc
     3481 ccggtccgaa ttgacgcggc gatcggttct tgctcgattt gaaacgctgg ttgccccagc
     3541 cgcccttgcc gccgcgcgcc accagcagct ccatgtctgc tttggccatg tcgcccagca
     3601 cttcaccggt ttcggcatcg cgcacgatcg tgcccacggg caccatgacc gtcgtgtctt
     3661 tgccaccgcg gccggtcatg tcacgaccgc cacccggttc gccattttcg gcgcggaatg
     3721 tgcgctcgac ccggaaatcg accagcgtgt tgatgccttc gcgagcgcgg aacgtgacgc
     3781 tgccgccaag tccaccatcg ccgccatcgg ggccaccgaa gggcacgaac ttctcgcgcc
     3841 ggaaactgat gcagcctcgg ccgccgtttc ccgcctgaac gcgaactcgc gcctcgtcta
     3901 cgaatttcat tcctgcgacc ctttgcctaa gcttccgcgc actttgtcag gggctgcgcc
     3961 ccactcataa acgagaaacc cggctaagaa gccgggtttc tcgtacatca aatgtaatgg
     4021 ggcaacccta ctcggcgtcg atgctcacga aggtgtgctg ttcttcaccc ttcttcgcga
     4081 acttcacgcg gcccggcttg agcgcgaaca acgtgtggtc ggtgccgatg ccgacattgc
     4141 tgcccgcacg catggtggtg ccgcgctggc ggaccaggat gttgccggcg agcacggttt
     4201 cgccgccgaa cttcttcacg ccgaggcgct tgctgtgtga ttcgcggccg ttacgagtac
     4261 tgccgcctgc ctttttatgt gccatttgag cctagctcca actaactaat agggaaccag
     4321 cgcttaagcg ctgatgccgg tgatcttgac ctgcgtgtac tgctggcggt gcgtgccctg
     4381 gcgcaggtag tgcttgcgac ggcggaactt gacgatgact ttcttctcgc ccttgccgtg
     4441 cttttcgacg gtggccgtaa ccttgccgcc cttgagcaga ggagcgccaa ctttgatgtt
     4501 gtcgccgtca ccgatgagca ggacttcgcc gaactcaatc gtggagcccg ggtcgcccgc
     4561 gaggttttca accttgagga cggtgccctt ttcgactcga tactgtttgc cgcccgtggc
     4621 gatgaccgcg tacatcgtga tgactccgaa aaaaccgttc gggaccaaag ccttaaagcc
     4681 atgtgccatc ccgaagggac gcgcattcta cgtaccactc ccgcaccggt caaaccgccg
     4741 cctgaacctg atttattcca ctagaatcaa cgccctaaat gagctcaaaa gtcacccacc
     4801 ttcccacctc ccgcccggct tcccgcacta ccgacgtc
//
LOCUS       DASQJS010000406        10303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1204563, whole genome shotgun sequence.
ACCESSION   DASQJS010000406 DASQJS010000000
VERSION     DASQJS010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10303
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204563"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /gene="mobB"
                     /locus_tag="VEW08_15945"
     CDS             <1..556
                     /gene="mobB"
                     /locus_tag="VEW08_15945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HYJ42286.1"
                     /translation="GAPESRPGESENSSARVLSDEGLMLVVAIVGRQGSGKTTLIERL
                     IPALHGRGLTVSTIKHTHHHRIELDVPGKDSHRHRLAGASEVIVASDAGWARIAARPE
                     PASLPVLVAQLRPVDIVLVEGFKQLEGLRRVEVFRGEGQPLALADPGIAAVAVPGGVP
                     PAGFRGVTLSLEDTPALLEFILSA"
     gene            654..1532
                     /gene="htpX"
                     /locus_tag="VEW08_15950"
     CDS             654..1532
                     /gene="htpX"
                     /locus_tag="VEW08_15950"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HYJ42287.1"
                     /translation="MKRIFLLVFTNIAVMAVLALAIQVFGLDRALAQKGMNLTGLLVI
                     SAIFGFGGAFISLLISKWMAKRAMGVKVITNPANETERWLVNVVTQQAQAAGIGMPEV
                     GIFDSPDPNAFATGANKNSALVAVSTGLLRNMRRDEVEAVLGHEVSHVANGDMVTLTL
                     IQGVMNTFVFFLARIIGGIVDKVILKNERGNGIGYFVTVIVAQIVLGFLAGMVVAWFS
                     RQREFRADAGGAHLAGTNSMIGALEALKRVHTPTALPEQMKAFGINDAAPTGLQRLLM
                     SHPPLDERIAALNSAR"
     gene            1653..2630
                     /locus_tag="VEW08_15955"
     CDS             1653..2630
                     /locus_tag="VEW08_15955"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HYJ42288.1"
                     /translation="MNSQTQTSNNPLVRLGSAVLAWFDTGRASSFDTQADHAGADRID
                     WVRTLPFILMHAACLFAFVVGVSPIAIVVCAALYFIRMFAITGFYHRYFSHKSFKTSR
                     VGQFLFGVLGASAVQRGPIWWAAHHRDHHQYSDKPEDAHSPIQHGFLRAHMSWFLSKK
                     GFAPDLKRVRDLMNFPELRWLDRFDIIVPVLLAVAVFFLGVALEKFAPGLGTNGWQML
                     VWGFFVSTVLTSHGTYTINSLSHVFGTQRYKTGDSSRNNWFLALITLGEGWHNNHHHY
                     PSSTRQGFYWWEVDITYYLLKLMSWMGIIWDLKPVPRSMRDDHPRRIVK"
     gene            2706..4004
                     /locus_tag="VEW08_15960"
     CDS             2706..4004
                     /locus_tag="VEW08_15960"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYJ42289.1"
                     /translation="MRVAVVGSGISGMVAASRLHRSHEVTVFEAGSHVGGHTNTIDVD
                     WQGRHFAIDTGFIVFNDWTYPRFIALLNELGVDYQDSNMSFSLRDERNGLEYNGTSVN
                     TLFAQRLNALRPSFLRMIADILRFNKRSLQLLAGNDDALTLGDYLDAHRYSRAFREQF
                     IVPMGMSIWSATERAMLSFPARFFVEFFDKHGFLSVNNRPVWQAIKGGSREYMRKLAA
                     PLAHRIRLNTAVTSVRRDWAGVTLRTGINTTERFDHVFFACHSDQALKILQDPSEAET
                     AVLGAIPYQENEAVLHTDDRLLPRTRLARAAWNYHLLAVRDSAMRDRIALTYDMNVLQ
                     SLDAPVRFLVTLNRTVDIDPAKVLKTITYHHPVYLPAGVAAQRRRADISGVNRTFYCG
                     AYWRYGFHEDGVVSAEWALEDFARFAQGASAESERLARAV"
     gene            4011..4796
                     /locus_tag="VEW08_15965"
     CDS             4011..4796
                     /locus_tag="VEW08_15965"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HYJ42290.1"
                     /translation="MKSALYSGWIRHRRFGPAQNRFRYRIFMSYLDLAELPGLLDKHW
                     FWSARRPAPAWFRRQDFLGPAQVPLDQAVRDLVQQRTGVRPAGPIRLLTHLRFFGYSF
                     NPVSFYYVFDAKDMRVETIVAEITNTPWKERHAYVLPVASARRGTGRAWRFSFEKQFH
                     VSPFLPMDMRYEWCFGAPSQELHVHMENWREGEACFDATLTLRRQSMNARAMSLALLQ
                     FPLITLKVSALIYWQALKLLVKRAPFHAHPRKRAKPAKSVMPA"
     gene            4793..6133
                     /locus_tag="VEW08_15970"
     CDS             4793..6133
                     /locus_tag="VEW08_15970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HYJ42291.1"
                     /translation="MNTRSPLLRETLDSPHPRRTTLLARLGRKLLLNQLRRFEHGEVR
                     LVEANGRQHVFGCRHAGFDLGCTVYFDHEQAFADAAFGGTVGAGEAYIRGLWRCDDLV
                     TLVRIFVANREQMNRLDSGWSLLSRPLLKLFHWANRNSRNGSARNIAAHYDLGNALYE
                     IMLDDTMAYSCAIFPRADATLAEASEAKFDAICRKLDLKPGDHVLETGTGWGGFAIHA
                     AQHYGCRVTTTTISVEQRDFAVRKIAALNLSDRITVLLHDYRDLTGQYDKLVSIEMVE
                     AVGAPFLDGYFSHCARLLKPEGAMLLQAITIQDQFYEQALKSVDFIQRHVFPGSFIPS
                     VQALSKSLSKATDLKIFHLEDIGPHYARTLRLWRERFFRNLATVRKLGYSESFVRLWE
                     YYLCYCEGGFTERQLGTVQMLLTKPGFRGGVRITASSGKPAMPRSPPAAVAGRS"
     gene            complement(6066..7385)
                     /locus_tag="VEW08_15975"
     CDS             complement(6066..7385)
                     /locus_tag="VEW08_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42292.1"
                     /translation="MSGKFLLILLALLVTTVVTAGDSAPVQHTRTCGGESCEAVFRGF
                     FTFFDRSLHGLDGNGRSCADCHMPSDQFQMSPAGVEARFQLLQKRRRWNPFADDPLFR
                     PVDADDFRTNGNKARDFRNLRENGLVRVQFTLPQNFRLIDPVTNLPSAESTVDIWRMV
                     PTVNDVKLTGPDGKNPWARAPNASGGYQLDGRFADLQEQALGALLDHAQVQAPPPQRM
                     LDDLAAFQRTLFTNHRVRALSDAISAGVSVLPDPDPPLSGLEQAGKVVFARSCGQCHG
                     GPGQSTTQAPVVRYHDILTQCPRPVDTVSPARFNFKPCPERLARNARTYEITLPNGTT
                     VRRVSSDPGRALLTGFVGGPPPLDDWNKLDVNGLRGIRHTAPYFHNNSAASLEEVVEH
                     YVEFYKRVRAGAAPGVVPPVASTDGINFDRQPQPEEIEALLAYLKKL"
     gene            7490..9397
                     /locus_tag="VEW08_15980"
     CDS             7490..9397
                     /locus_tag="VEW08_15980"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HYJ42293.1"
                     /translation="MNIDVVAPYLELPGFTVDVRRRLLLRRESGEVIALNAKVFDTLL
                     YLAQHPGEVLDKDTLLSAVWPGVVVEENSLAQNVSALRQALGETHGENRYIATVPRRG
                     YRFVAEVHARSELPAASDTPVAAHSGTSSPRAIPRPWWIGAMVLAVALLIGATWFWRA
                     GEREEPAATPGALPALAVLPFKPLVLTERDESLELGMTDSLIAKLSELDGHVVRPLSS
                     VRRYAAPEQDALAAARALDAQVVLDGFLQHSGGRLRVTTRLLDVKDGRQIWAGSFDET
                     FTTIFEVQDTIARRVSEALRAPLSDRAARRSERGSTQNSAAYLAHANGKLAWSRLTRQ
                     SLNQAIGEFEKALALDPRYALAYVGIADSYATLGVFGVMAPHDAFPRARRAAQQALAI
                     ESGLAAAHATLGHITVQYDLDWITGMAEYERAIALDPGYARTYHWRGLSNAMHGATAR
                     AIADFEHARQLEPLWIAPRAATGNILFYARRYKEAIAELSATLELDERADNARTYRAR
                     AYLHSGQRDLALAEFLKLREREARTPGSFGDVGQALALLGRTDEARAELARLVQLATT
                     RYVPALDIATIHASLGDRDSAFQWLERAYTDRSTNISMLEYDPSFDALHDDARFAALV
                     ERIDQRKRKDL"
     gene            9432..>10303
                     /locus_tag="VEW08_15985"
     CDS             9432..>10303
                     /locus_tag="VEW08_15985"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYJ42294.1"
                     /translation="MNDTAALTRSLHELLDDAGVLTDPVRTAPMLRDHRALYRGHALA
                     VVAPADTAAVARVLAYCNEHRIGVVPQGGNTSYCGGATPDASSSQVLLSLARLNRVRA
                     VDADNYTMIAEAGCILASLQAAAADADRYLPLSLGSEGSCMLGGNLSTNAGGLNVIHY
                     GMARELVLGLEVVLADGRVMDTLTGLRKDNTGYDIKSLFLGAEGTLGIITAACIKVYP
                     APRTRATAFIAARDPAAAVSLLGALRQASGDCVTSFELIPRIGIELTTRHIPGVTDPL
                     SAPSPWYVLAELSSA"
BASE COUNT         1816 a         3422 c         3199 g         1866 t
ORIGIN      
        1 gggggcgccg gagtcgcggc ctggggagtc ggaaaactcc tcggcgcgag tattgtcgga
       61 tgagggcctg atgctcgtcg tcgctattgt cggacgtcag ggcagcggca aaacaacgct
      121 catcgaacgg ctgatccccg cgctgcacgg tcgcggcctc acggtctcga ccatcaaaca
      181 cacccaccat caccgcatcg agctcgacgt acccggcaag gacagccacc gtcaccgcct
      241 ggcgggggcg tccgaggtca tcgtcgcttc ggatgcgggg tgggcacgca tcgccgcaag
      301 gccggagccg gccagcctgc cggtgctggt ggcgcagctg cgccccgtcg acatcgtgct
      361 ggtcgaggga ttcaagcaac tcgaaggttt acgacgggtc gaagtgttcc ggggcgaagg
      421 ccagccactg gcgctggccg atccgggcat cgcggccgtg gcggtgccag ggggcgttcc
      481 gccggccggt ttccgcggcg tgacactgtc gctcgaggac acgccagcgc tgctggagtt
      541 catcctaagc gcttgatttg aaagtattgc catcccggca ttgaattcgc cgtggccgac
      601 gccagaattg ccgcagccga tttgacgtga acccaacagg agccgtcgct tagatgaaac
      661 gcatattcct tctcgtcttt accaacatcg ccgtcatggc ggtgctggcc ctggccatcc
      721 aggtgtttgg cctcgatcgc gcgctggcgc agaagggcat gaacctcacc ggcctgctcg
      781 tcatctcggc gatcttcggc ttcggcggcg ctttcatctc gctgctcatc tccaagtgga
      841 tggccaagcg cgccatgggc gtcaaggtca tcacgaaccc ggccaacgag accgaacgct
      901 ggctggtgaa tgtcgttacc cagcaggccc aggcggctgg catcggcatg cccgaagtcg
      961 gcattttcga ttctccggat ccgaacgcgt ttgccacggg tgctaacaag aactccgcgc
     1021 tggtggcggt gagcacgggc ctgcttcgta acatgcgccg cgatgaagtc gaggccgtgc
     1081 tgggccacga agtgagccat gtcgccaacg gcgacatggt cacgctcacg ctcatccagg
     1141 gcgtcatgaa caccttcgtg ttcttcctcg cgcgaatcat cggcggcatc gtcgacaagg
     1201 tcatcctcaa gaacgagcgt ggcaatggca tcggctactt cgtcacggtg atcgtcgcgc
     1261 agatcgtgct cggcttcctc gccggcatgg tcgtcgcctg gttctctcgc cagcgtgagt
     1321 tccgtgcgga cgcgggtggc gcacatctcg cgggcaccaa ttcgatgatc ggcgcactcg
     1381 aagccctcaa gcgtgtccat acccctacgg cgttgccgga gcagatgaag gccttcggaa
     1441 tcaacgacgc cgcgcctacg ggcctgcagc ggctgctcat gagccatccg ccgctcgatg
     1501 agcgaattgc cgcactgaac agcgcgcgct aactgccaag cacttcaaag ccggcgccgg
     1561 gcggaatgct gcaacattcc ccggcgccgc cgttgggagc gcatccgatt ccacccgacg
     1621 ccgtatcacc tgagaaggaa ttcgcgaatt acatgaactc gcagacgcaa acgtcgaaca
     1681 atccgctggt gcgcctcggg tcagcggtgc tggcctggtt cgacacgggc cgggcctcga
     1741 gcttcgacac gcaagccgat cacgccggcg ccgatcgcat cgactgggtt cgcacgctgc
     1801 ctttcatcct gatgcacgcg gcgtgtttgt ttgcgttcgt ggtcggggtg agtccgatcg
     1861 ccatcgtcgt ttgtgccgcc ctgtacttca ttcgcatgtt cgcgatcacc ggcttctatc
     1921 accgctattt ctcgcacaag tccttcaaga cctcgcgcgt cggccagttc ctgttcggcg
     1981 tgctgggcgc ctcggccgtg cagcgcggcc cgatctggtg ggcggcacat catcgcgacc
     2041 accaccagta ttccgacaag cccgaagacg cgcattcgcc catccagcac ggattcctgc
     2101 gcgcgcacat gagctggttc ctttcgaaaa aaggtttcgc gccggacctg aagcgcgtgc
     2161 gcgatctcat gaattttccc gagctgcgct ggctcgaccg tttcgacatc atcgtgccgg
     2221 tgttgctggc ggtggcggtg ttcttcctcg gcgtggcgtt agagaagttc gccccgggtc
     2281 tcggcaccaa cggctggcag atgctcgtct ggggcttctt cgtatccacc gtgctcacgt
     2341 cgcacggtac gtacaccatc aattccctgt cgcacgtctt cggcacgcag cgttacaaga
     2401 cgggtgattc gagccgcaac aactggttcc tcgcgttgat cacgcttggc gaaggctggc
     2461 acaacaatca ccaccactac ccgagctcga cgcgccaggg cttctactgg tgggaagtcg
     2521 acatcaccta ttacctgctc aagctgatgt cgtggatggg gatcatctgg gacctgaagc
     2581 ccgtgccccg ctcgatgcga gatgatcatc cgcgccgaat cgtcaaatag cccttagttt
     2641 tccgcgcatc cgaatgacct gaactcccgt aggtacgggg gagttcggtc actttcggat
     2701 tttccatgcg cgttgcggtc gtaggttctg gcatctccgg catggtggcc gcgtcgcggc
     2761 tgcatcgaag ccatgaggtc acggttttcg aagccggttc ccatgtcggc ggacacacaa
     2821 acaccatcga cgtcgactgg cagggccgcc actttgcgat cgacaccggc ttcatcgtct
     2881 tcaacgactg gacgtatccg cgtttcatag ctctcttgaa cgagctcggc gtcgactacc
     2941 aggactcgaa catgagcttc agtctgcgcg atgaacgcaa cgggctcgaa tacaacggca
     3001 ccagcgtcaa cacgctgttc gcccagcggc tgaatgcgct gcgcccgtcg tttctgcgca
     3061 tgatcgccga catcctgcgc ttcaataagc gcagcctgca gctcctggcc gggaatgacg
     3121 acgcgcttac gcttggcgac tatctcgacg cgcatcgcta ttcgcgcgcc ttccgggaac
     3181 agttcatcgt tcccatgggc atgtcgatct ggtcggcaac cgagcgggcg atgttgtcgt
     3241 tcccggcgcg attcttcgtc gagttcttcg acaagcacgg ctttctgagc gtgaacaacc
     3301 gccccgtgtg gcaggcgatc aaaggcggct cgcgcgagta catgcgaaag ctggcggcgc
     3361 cgctcgcgca tcgaatccgg ctgaacaccg ccgtcacatc cgtgcgtcgc gattgggcgg
     3421 gcgtcacgct tcgcaccggg atcaacacga ccgaacgttt cgaccacgtg ttcttcgcct
     3481 gccacagcga ccaggcgctg aagatcctgc aagacccgtc cgaggcggag accgccgtgc
     3541 tcggcgcaat tccctaccag gaaaacgaag ccgtgctgca caccgatgac cggcttctgc
     3601 cgcgcacacg gctcgcgcgc gcggcctgga actatcactt gcttgcggta cgcgacagcg
     3661 ccatgcgcga ccggatcgcc ctcacctacg acatgaacgt gctgcagtcg ctcgatgcgc
     3721 cggtgcgttt cctggtgact ctcaaccgca ccgtggacat cgatccggcg aaggtgctga
     3781 agaccatcac ctaccaccat ccggtctatc taccggccgg cgtggcggca cagaggcgcc
     3841 gcgccgacat cagcggcgta aatcgcacgt tctactgtgg cgcttactgg cggtatgggt
     3901 ttcacgaaga tggcgtggtc agcgccgagt gggcgctcga agacttcgct cgcttcgcgc
     3961 aaggcgcgag cgccgaatcc gaacggctgg cccgcgccgt ataacctcac atgaaaagcg
     4021 ctctatattc gggatggatt cggcatcggc gcttcgggcc ggcgcagaac cgctttcgat
     4081 atcgcatctt catgagctac ctggatctcg cggagttgcc gggactgctc gacaagcact
     4141 ggttctggtc ggcgcgccgg cccgcgcctg cctggtttcg ccgccaggat tttctgggcc
     4201 cggcgcaagt gccgctcgat caggcggttc gcgatctcgt gcagcagcgc accggggtgc
     4261 ggcccgcggg cccgatccgc ctgctcaccc atctgcgttt tttcggttac agcttcaacc
     4321 cggtgagctt ctactacgtg ttcgacgcga aggacatgcg cgtcgaaacc atcgtcgccg
     4381 agatcaccaa cacgccgtgg aaagagcgtc acgcctacgt gttgccggtc gccagcgcgc
     4441 gccgcggtac gggtcgcgcg tggcgcttca gcttcgagaa gcagtttcac gtctcaccat
     4501 tcttgcccat ggacatgcgc tacgagtggt gtttcggcgc gccatcgcag gagctgcacg
     4561 tccacatgga aaactggcgc gaaggcgagg cctgcttcga cgccacgctc actctgcggc
     4621 gccagtccat gaacgcgcgc gcgatgtccc ttgcgctcct gcaatttcca ttgatcacgt
     4681 tgaaggtctc ggcgctcatc tactggcagg cgctgaagct gctggtgaag cgggcgccat
     4741 tccatgccca tccgcgcaaa cgcgcgaagc ctgccaaatc ggtgatgccg gcatgaacac
     4801 ccgctcgccc ctgctacgcg aaactctcga ttcacctcac ccgaggcgca cgacgctgct
     4861 ggcgcgtctc ggccgcaaac tgctgctcaa ccagcttcgc cggttcgaac acggcgaagt
     4921 gcgcctggtc gaagccaatg gccggcaaca tgtgttcggc tgccgtcacg ccggattcga
     4981 tcttggctgc acggtgtact tcgaccacga acaggctttc gccgacgcag ccttcggcgg
     5041 cacagtcggt gcaggcgagg cgtacatccg cgggctgtgg cgctgcgacg acctcgtcac
     5101 gctggtgcgc atcttcgtgg ccaatcgcga gcagatgaat cgcctcgact ccggctggtc
     5161 gctgctgagt cgcccgctgc tcaagctctt tcactgggcc aaccgcaaca gcaggaacgg
     5221 cagcgcgcgc aacatcgccg cgcactacga cctgggcaat gcgctctacg aaatcatgct
     5281 cgatgacacc atggcctatt catgcgcaat tttcccgcgc gccgacgcca cgctcgcgga
     5341 agcatcggaa gcgaagttcg acgcgatctg ccgcaagctc gacctgaaac ccggcgacca
     5401 cgtgctcgag accggcacgg gctggggtgg gttcgccatt cacgccgcgc agcactacgg
     5461 ctgccgcgtc accaccacca cgatctcggt ggagcaacgc gacttcgccg ttcgcaagat
     5521 cgccgcgctg aacctttccg atcggatcac ggtgctgctg catgactatc gcgacctcac
     5581 ggggcaatac gacaagctcg tctccatcga gatggtggag gcagtcggcg cgccgttcct
     5641 cgacgggtac ttcagtcact gcgcgcggct gctcaagccc gaaggcgcca tgctgctgca
     5701 ggccatcacc atccaggacc agttttacga gcaggcactg aaatcggtgg acttcatcca
     5761 gcgccacgtg tttcccggaa gtttcatccc ttcggtgcag gcgctgtcga agtcattgtc
     5821 aaaggcgact gatctgaaaa tcttccacct cgaagatatc ggcccgcact acgcgcgcac
     5881 gctgcgcctg tggcgagagc ggtttttccg caacctcgcc acggtgcgca aactcggcta
     5941 cagcgagagc ttcgtgcggc tgtgggagta ctacctttgt tactgcgaag gcggtttcac
     6001 cgaacgccag ctcggcaccg tgcagatgct gctgaccaag ccggggtttc ggggcggcgt
     6061 ccgcatcaca gcttcttcag gtaagccagc aatgcctcga tctcctccgg ctgcggttgc
     6121 cggtcgaagt tgattccgtc cgtggacgcc acgggcggca ccacaccggg cgccgcaccg
     6181 gcgcgcacgc gcttgtagaa ctcgacgtag tgctcgacca cttcttccag cgatgccgca
     6241 ctgttgttgt ggaagtacgg cgcggtgtgg cggatgccgc gcagaccgtt cacgtccagc
     6301 ttgttccagt catcgagcgg cggaggccct ccaacaaacc cggtcagtag cgcgcggcca
     6361 ggatcggaac tgacgcgacg cacagtggtg ccgttgggga gagtgatctc atatgtgcgc
     6421 gcgttgcgcg ccaggcgctc gggacaaggt ttgaagttga aacgggctgg cgacaccgtg
     6481 tccaccgggc gggggcattg agtcaggata tcgtggtaac gcaccacggg cgcctgcgtg
     6541 gtggattggc caggaccgcc atgacattgg ccgcaggaac gcgcgaacac gaccttgccc
     6601 gcctgttcga gtcccgagag cggaggatcg ggatcgggaa gaactgaaac ccctgcagag
     6661 atcgcatcgc tgagcgcccg cacacgatga ttggtgaaaa gcgtgcgctg aaaggcggcg
     6721 aggtcatcga gcatgcgttg cggcggcggc gcctggacct gcgcgtggtc cagcagtgcg
     6781 ccaagggcct gctcctgcag atccgcgaac cgtccatcca gctgatatcc gccgctggcg
     6841 ttgggcgcgc gcgcccaggg attcttccca tccgggccag tgagcttcac atcgttgacc
     6901 gtcggcacca tgcgccagat gtccaccgtg gattccgctg acggcagatt ggtgaccgga
     6961 tcgatcaggc ggaagttctg cggcagcgtg aactgcacgc gcaccaggcc gttctcgcgc
     7021 aggttgcgga agtcccgcgc cttgttgcca ttggtccgaa aatcgtcggc gtcgacgggc
     7081 ctgaacagcg gatcgtccgc gaaagggttc cagcgccgtc gcttctgcag cagctggaac
     7141 cgggcttcca cgcccgccgg tgacatctga aactggtccg acggcatgtg acagtcggcg
     7201 caggatcgtc cgttgccatc gaggccatgc aaggaacgat cgaaaaaggt aaagaacccg
     7261 cgaaacacgg cttcgcacga ctcaccgccg caggttcgcg tatgttgcac cggcgctgag
     7321 tcgccggccg tcaccacggt ggtcaccagc aaggccaaca ggatgagaag gaatttcccg
     7381 gacatatgtt tctcccttta cgcttgcgaa aattgctgcc agggaatctc gccgcccgtc
     7441 aggcgaaaat catgggcggt tcgatgaaaa atcctgaatt gttgtgttca tgaacatcga
     7501 tgtggtcgcc ccgtacctgg aattgcccgg tttcaccgtg gatgtgcgcc gaaggctgct
     7561 gctgcgtcgc gaaagcggcg aggtcatcgc gctcaacgcc aaggtcttcg acacgctgct
     7621 gtacctcgcg cagcatcccg gcgaggtgct ggacaaggac accttgctca gcgcggtatg
     7681 gccgggcgtg gtcgtggagg aaaacagcct ggcgcaaaac gtttccgcgc tccgccaggc
     7741 gctgggcgag acccatggcg agaaccgtta catcgcaacc gtgcctcgtc gtggttatcg
     7801 gttcgtagcg gaggtccatg cgcgcagtga attgccggcg gcaagtgaca cgcccgtcgc
     7861 tgcgcattcc ggaacatcct caccgcgcgc catcccccgg ccctggtgga taggggcaat
     7921 ggtcctggct gtggcgctgt tgatcggcgc cacctggttc tggcgcgcgg gtgaaaggga
     7981 agaacccgca gcaacgcccg gcgccttgcc ggcgctcgcc gtgctgccgt tcaagccgct
     8041 ggtgctgacc gaacgcgacg aatccctcga gctcggcatg accgattccc tgatcgcgaa
     8101 gctcagtgag ctcgacggtc acgtcgtgag gcctctgagc tccgtgcgcc gctatgcggc
     8161 gccggaacag gacgcgctgg ccgcggcacg cgctctggat gcacaggtgg tgctggacgg
     8221 attcctgcag cacagcggcg gccggttacg cgtgaccacg cgactgctgg atgtgaagga
     8281 cggtcgccag atctgggcgg gaagcttcga cgagaccttc accaccatat tcgaagtgca
     8341 ggacaccatc gcccggcggg taagcgaggc gctgcgggcg ccgctgtcgg atcgcgcggc
     8401 gcgacgcagt gagcgcggct cgacacagaa ctcggcggcc tatcttgcgc acgcgaatgg
     8461 caagctggcc tggagtcgcc tcacccgcca aagcctcaac caggccatcg gcgaattcga
     8521 aaaagccctg gcgctcgatc cacggtatgc cctcgcctac gtgggcatcg ccgacagtta
     8581 cgcgacgctg ggcgtgttcg gcgtgatggc cccgcacgat gcgttcccgc gcgcacgccg
     8641 tgccgcgcag caggcgctgg caatcgagtc cgggctcgct gccgcgcacg ccacgctggg
     8701 gcacatcacg gttcagtacg atctcgactg gatcaccggc atggccgagt acgagcgcgc
     8761 catcgcgctc gatcccggct atgcgcgcac ctatcactgg cgcggcctct cgaatgccat
     8821 gcatggcgcc accgcgcgcg ccatagccga cttcgaacac gcgcggcagc tggagccact
     8881 gtggatcgcg ccgcgtgcag ccacgggcaa tatccttttc tacgcacgcc gttacaagga
     8941 agccatcgcc gaactcagcg cgacgctgga actcgacgag cgcgccgaca acgcccgcac
     9001 ctatcgtgcg cgggcctatc tacattcggg gcagcgcgac ctggcattgg ccgaattcct
     9061 gaaactgcgc gagcgcgagg cgcgcacgcc gggcagcttc ggcgatgtcg gccaggcgct
     9121 ggccctgctg ggacggaccg acgaagcgcg cgcggaactg gcgcggctgg tgcagctcgc
     9181 gacgacccgc tacgtgccgg cgctggacat cgccaccatc catgccagcc tgggcgatcg
     9241 cgacagcgcg ttccagtggc tggaacgggc ctataccgac cgctccacca acatttccat
     9301 gctggagtac gacccgagct tcgacgccct gcacgacgat gcgcgcttcg ccgcgctggt
     9361 ggaacggatc gaccagcgca aacgcaagga cctgtgaagt caagccagac cccacgcgct
     9421 atcatgcgcg cgtgaacgac accgccgccc tcacccgctc gctccacgaa ctgctcgacg
     9481 acgccggcgt tctcaccgac ccggtccgaa ccgcgcccat gctgcgcgat catcgtgcgc
     9541 tgtatcgcgg ccatgcattg gcagtggtgg cgcccgccga caccgccgcc gtggcgcgcg
     9601 tgctcgccta ctgcaatgag caccgcatcg gcgtggtgcc ccagggcggc aacaccagct
     9661 actgcggtgg cgccaccccg gacgcgtcca gcagccaggt cctgctgtcg ctggcgcgcc
     9721 tgaaccgcgt acgcgccgtc gacgccgaca actacacgat gattgccgag gccggctgca
     9781 tcctggcgag cctccaggcc gcagccgccg acgccgatcg ctatctaccg ctgagcctgg
     9841 gaagcgaagg cagctgcatg ctcggtggca atctctccac gaacgccggc ggcctcaacg
     9901 tcatccacta cggcatggcc cgcgagctgg tgctgggcct cgaagtggtg ctcgccgacg
     9961 gccgcgtgat ggacacgctc accgggctgc gcaaggacaa cacgggctac gacatcaagt
    10021 cgctgttcct gggcgccgaa ggcacgctcg gcatcatcac ggccgcgtgt atcaaggtct
    10081 acccggcgcc gcgcacgcgc gccaccgcat tcatcgccgc gcgcgatccg gcggccgccg
    10141 tatcgttgtt aggtgcgttg cggcaggcca gcggcgactg cgtcacctcg ttcgagttga
    10201 tcccgcgcat cggtatcgaa ctcaccacgc gccacatccc gggcgtgacg gatccgctgt
    10261 ccgcgccctc gccctggtac gtcctggcag agctttcctc tgc
//
LOCUS       DASQJS010000407        26043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2149951, whole genome shotgun sequence.
ACCESSION   DASQJS010000407 DASQJS010000000
VERSION     DASQJS010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 26043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26043
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2149951"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VEW08_15990"
     CDS             <1..1177
                     /locus_tag="VEW08_15990"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HYJ42295.1"
                     /translation="AIDASDSLELDTVDKKVYWDVVDHCYLCDMCFMTKCPYVPPHPW
                     NIDFPHLMLRAKHVQHRKGEHSFRDKLLSNTRGLGSIAGIPVVAEIVNAVNGTRVGRK
                     LLEATLGVDATAPIPKFHSNTGTKRARRESGKATEVTPTEETRGKVALFVTCYGNRNE
                     PHMPEDLAVVFEHNGIPVKVVGKEKCCGMPKLELGDLETVEKYKNFNIPQLKALIDDG
                     YDIVAAIPSCVLMFKQELPLMFPDEADVQMVKEHIFDPFEYLMLRHKAGLLRTDFTAR
                     LGKVSYHVPCHLRVQNIGLKTRDVLRLVPETTVDVIERCSGHNGTYAVKKEFRAASVK
                     IGRPVVSKVEQAKADHYSSDCPMAGHQIESGLSPDASGAGPREPEHPMTLVRKAYGL"
     gene            1183..1767
                     /locus_tag="VEW08_15995"
     CDS             1183..1767
                     /locus_tag="VEW08_15995"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HYJ42296.1"
                     /translation="MHKLAESDLLSLERYSRERLDFRARVMAHKRNRQVNVGPNTMWL
                     FEDRLTVQYQVQEMLRTERIFESEGIAEELAAYNPLIPDGRNWKVTFLIEYTDPEVRR
                     VQLERLMGVEDACWVQVAGFHRVFAIADEDLERSNEVKTSAVHFLRFELSEPMASGLK
                     GGAALSIGIDHPNYQHQVSPAADNVRAALAADLG"
     gene            complement(1791..2201)
                     /locus_tag="VEW08_16000"
     CDS             complement(1791..2201)
                     /locus_tag="VEW08_16000"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich outer membrane protein"
                     /protein_id="HYJ42297.1"
                     /translation="MNVFWIPIIAIVFSLSFAMLGLWADHQRRSQKLEHAHRERMAAI
                     EKGVPLPVIAEERDRADDKWSNPARMLRSGVLLLTLGIILYFAIHAAGGVEGALFGLI
                     PSALGLANLAYAAVLFDREKKAAAQKENSSLPPL"
     gene            2372..2944
                     /locus_tag="VEW08_16005"
     CDS             2372..2944
                     /locus_tag="VEW08_16005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYJ42298.1"
                     /translation="MERDADLVAALRQGDRRAVLEGLMTRYRQKVMNLAVSIVRDQAL
                     AEDLSQTVFVKVWQALPKFDGRASISTWLYTITRNTCLTAVTRERRLVPLEDFGEVAD
                     DDGDPSVFGTAVAGQEDASQVAAEYDVAKLLEQLPEPYRRVVVLFYLEDRSCEEVGEL
                     LSMPTGTVKALLHRGRKRLAALAGEMENES"
     gene            2941..3348
                     /locus_tag="VEW08_16010"
     CDS             2941..3348
                     /locus_tag="VEW08_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42299.1"
                     /translation="MKSNPENEAILERSLRKQVNVRQLDDSFDAAVWARIEAEESRSM
                     AQAPVRAATSSKVGRWLFILNAVGIASVAVFLCVFLFQWLAGVQVDATFANSSFRVSD
                     QTVTIWASGIAGAALLAGFFYTPWGGRLRDEFF"
     gene            3451..5343
                     /gene="parE"
                     /locus_tag="VEW08_16015"
     CDS             3451..5343
                     /gene="parE"
                     /locus_tag="VEW08_16015"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HYJ42300.1"
                     /translation="MSYNASDIEVLSGLEPVRRRPGMYTDTSRPNHLAHEVVDNSVDE
                     ALGGHAKEINVILHKDNSLSVADDGRGMPVDIHPKEKVSGVELILTRLHAGGKFNDKN
                     YEVSGGLHGVGVSVVNALSKQLETWIKRNGKEYNISFKDGKVSSKLEVVGTVGQRNTG
                     TTVRFWPDPKFFDSDKFSVPQLRHVLKAKAVLCPGLKITFENEATKEKDEWFFTGDLA
                     AYLKEEIGKEVPLPAEPIFAKNDQQKINVVEYAIVWTPNADRAMGESYVNLIPTSEGG
                     THVNGLRSGVAGAVREFCEFRNLVPRGLKLSPEDIFDKCCFVLSIKIQEPQFAGQIKE
                     RLASRDAAPLTESFVHDSLSLWLNQHPDAGERIAQFAIENAQERTKAAQQVKRKRVTA
                     GPALPGKLADCTNQDPKHSELFLVEGDSAGGSAKQARDRETQAIMPLRGKILNTWEVS
                     VGEINSSQEVHDISVAMGIDPGTSDLSGLRYHKICILADADSDGLHIATLLCALFLRH
                     YRPLVANGHVFVAMPPLYRIDAGKNIYYALDDAERDSLLKKIENEGKTKAKPHVQRFK
                     GLGEMNPSQLRETTMDPRTRRLVQLTISAKDDSDSIMDMMLAKKRASDRREWLEEKGN
                     LAEVIP"
     gene            5358..7595
                     /gene="parC"
                     /locus_tag="VEW08_16020"
     CDS             5358..7595
                     /gene="parC"
                     /locus_tag="VEW08_16020"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HYJ42301.1"
                     /translation="MQDELNFEGVEKQPLRTFTEKAYLDYSMYVILDRALPALSDGLK
                     PVQRRIIYAMSELGLSSGQKHKKSARTVGDVIGKFHPHGDSACYEAMVLMAQDFSYRY
                     PIVDGQGNWGSPDDPKSFAAMRYTESKLTRYAEVLLSELSDGTVDWQPNFDGTLEEPA
                     FLPARLPNVLLNGTQGIAVGMSTDIPPHNLREIAQACIHLLDEPDAMTRTLMKYVKGP
                     DLPTGGEIVSPRSEILDIYNTGNGSFKARAVYKQEDDNIVISELPYQVSGNKVIEQIA
                     AQMREKKLPMVEDIRDESDHEERIRIVIEPKKRTDVAQLMAHLFATTDLERNYRVNIN
                     VITLEGKPKVMGLRDLLNEWLVFRTTTVTRRLQFRLDKVAARLHILDGLLAAYLNLDE
                     VIRIIRREDEPKPVLMKRFKLTEIQAEAILETKLRHLAKLEEMKIRDEQKKLAEEREE
                     LEGILKSKAKLKKLVASEIEADAEKYGDDRRTKIVEREAAQAIDESELVANEPATIVL
                     SKGGFVRSAKGHEIDARSLTYKAGDEYQHAAKGRSVQQAVFLDSTGRAYSVVAHSLPS
                     ARGLGEPLSSRVDPPDGAKWRGVMIGEPEDLWLVASDAGYGFTVRLKEAYASKKAGKT
                     LLNIPDGALVLPPAPVPGDDALVAVTSEGGKLLVFPVKDVPELARGKGNKLFDIPAKK
                     FASREDFLTGMAVVPKDKSLVVRSGERKMTLSWSDLKDYRGQRAQRGSVLPRGWRTVD
                     VLEVE"
     gene            complement(7609..8223)
                     /locus_tag="VEW08_16025"
     CDS             complement(7609..8223)
                     /locus_tag="VEW08_16025"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HYJ42302.1"
                     /translation="MTIYGLLTFCAVYALAVAAPGPGVAAVIARGLAHGLKGTPAFIA
                     GFMVGDLVWFAIAATGLAALAKTAATAFVVIKWIGVTYLLYLAWKLWSAPAERVTVKE
                     ADDRQHGWKAFLASFLLTLANPKTILFFVALLPTVVDIASMNLVRFVEISAAMCVVLP
                     AVLGAYAFLAARARELFTTPKSVRRLNRSSGVAMASAAVVVATR"
     gene            8293..9318
                     /locus_tag="VEW08_16030"
     CDS             8293..9318
                     /locus_tag="VEW08_16030"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HYJ42303.1"
                     /translation="MATPLRVTLVLLASMFAPGAYAQVAGSPPAGKTWALAIHGGAGV
                     IQRGALTKDKEVAYRAAMLTAMDAGSTVLRRGGSSLDAVEAAIRVLEDDPLFNAGRGA
                     VFTAEGRNELDASIMDGATRKAGAVAGVTRTRNPISLARAVMEKSPHVMLAREGADQF
                     AVEQGLPQVDPAYFRTEQRWQQLLDWRRDNAKLLDQTHSRGTVGAVAIDASGHLAAGT
                     STGGMTGKRWGRVGDSPVIGAGTYASDGSCAVSATGSGEYFIRASAARQICDRIAWRG
                     DSVQSATSATIADIGSIGGDGGVIAMDGKGNIAFAMNTSGMYRGWVTSTQSPATAIYS
                     GEEGPAR"
     gene            complement(9315..11438)
                     /locus_tag="VEW08_16035"
     CDS             complement(9315..11438)
                     /locus_tag="VEW08_16035"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ42304.1"
                     /translation="MTDNSAVPLIVFSPQREPSEFINSLMRKAGEPVHCTWIPAVRDL
                     GEALEQLNPELLVCAYVSDTDAKQAATVRDQLAPQVPMLLVRPQISEGDIATGMRMGA
                     RDVVSLSATDRVQAVVSRELRSFRLERALNSTLQSASDYRRQLQSVLQRSNDAILQVQ
                     EGIVVDANQTWLELFGYADAGVVVGQPLMDLFEDRSHAALKGALVACGQGKWTDHPLK
                     VTALLGDGTQLQLDLVLTPGEFDNEPCVRIMVPARKRDDRQLTTDLSDAVKRDATTGL
                     LLRRPLLDAIGVRIGAPPAGGVRFMALVRPDKFAELEKDIGARASEQLLSEYAAMLKS
                     HLGPNDIAGRFAGVSFLALLERGNTHDVEAWAEQVLARFSESVSTVGQKTVHSSCTIG
                     LTTVPPAGSNIDAIVADVVEAVRRARKEGGNQIFHLDKADTDTRVQAYDKIWVKHIKS
                     ALMENRFRLVQQPVASLQGDDPNMFDVLVRMLDHQNKEVLPAEFMAAAERNDLLKNID
                     RWVVGASLSFAAQKKPGALFVRLSRDSVLDAGFAEWLKTQLVTGTHKPDRICFQITES
                     IYEQYMNAAVAQLGALKKMGFRIALERFGSGRDPIKLLSTVPLDFIKIDGALVQDLAG
                     NFETQTVVKKLVEAAHKLKIETIAERVEDANTMAVLWQLGVQFIQGYFVNAPEDVTIT
                     DTQTLRTLSSSPAAATPVKPRPNPR"
     gene            11637..12206
                     /gene="efp"
                     /locus_tag="VEW08_16040"
     CDS             11637..12206
                     /gene="efp"
                     /locus_tag="VEW08_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HYJ42305.1"
                     /translation="MSSYTTNEIRGGMKLLIDGDPYAVIDNEYVKPGKGQAFNRIKVR
                     NLKTGRTVEKSYRSGESIEAADVLDSEMQYLYKDGDFWTFMNPENYEQLQAGEAAVGD
                     AAKWLKDGVVCIITLWNGVPLVVTPPPHIDLKVIETDPGLKGDTATGGQKVAKLETGA
                     SVRVPLFINEGELLRIDTRSGEYVSRAKG"
     gene            complement(12207..12695)
                     /locus_tag="VEW08_16045"
     CDS             complement(12207..12695)
                     /locus_tag="VEW08_16045"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYJ42306.1"
                     /translation="MSHATQHYDFSDADAAYRELARDLAALLAGERDLIANAANTAAL
                     IYDALPGLNWAGFYLYKSGELVLGPFQGKPACVRIAMGKGVCGTAAARRETVIVEDVH
                     AFPGHIACDSASNSEIVIPLLRGPELLGVLDLDSPLHARFGAADARGLEALAKIFLKS
                     LD"
     gene            complement(12696..13661)
                     /gene="epmA"
                     /locus_tag="VEW08_16050"
     CDS             complement(12696..13661)
                     /gene="epmA"
                     /locus_tag="VEW08_16050"
                     /inference="COORDINATES: protein motif:HMM:NF006828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HYJ42307.1"
                     /translation="MSDATWRPTATTRALRQRASALRATREFFFSRQVLEVETPAMIH
                     APVSDVNLGSVRVQMSGTEDPLFLHTSPEYAMKRLLAAGSGDIFQICHVYRGAESGRQ
                     HNPEFTMLEWYRLGFSLEQLMREVADLTNQLLGTRLAVEFVSYRDAVRRHAGFDPLDA
                     DSGELQRAAQSLGLDAAHAAQAGRDELLDLIVGAQVGPALGAATLTFLHRYPASQAAL
                     ARLDPDDPRVALRFELYHRGLELANGYHELTSGAEQRMRFAVDQQSRKKRGLPTFELD
                     AHLLAALDAGLPDCAGVALGFDRVLMLAMNAASIDEVIAFPTERA"
     gene            complement(13670..14575)
                     /gene="asd"
                     /locus_tag="VEW08_16055"
     CDS             complement(13670..14575)
                     /gene="asd"
                     /locus_tag="VEW08_16055"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HYJ42308.1"
                     /translation="MSSPGASGRAFVALQHLLPQHGISRLVLAATRSRAPAFKNALIR
                     LFVRGFNPDMNDAVQTEPTAYASFNEFFTRALRDGTRPVDADPEAIVSPVDGTVSEAG
                     ELSADRLLQAKGHEYSLRALLAGNSAWERTFAGGTFATIYLAPYNYHRIHMPLAGELR
                     EAFYVPGRLFSVNRTTAQLVPGLFSRNERVFCGFDSGGMPWANILVGALNVGSMATVW
                     HGDVTPRKVREVTALPVSEVLAPAQLAKGDEMARFNMGSTVILLFPPGAVRWDGNLKA
                     GNTLRMGERIGTLSGWRSTPPEQTR"
     gene            complement(14572..15198)
                     /locus_tag="VEW08_16060"
     CDS             complement(14572..15198)
                     /locus_tag="VEW08_16060"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYJ42309.1"
                     /translation="MSNRYWISVAAIAAAAALAGVYVARMLGQPAVASLESGTVFPRP
                     RPLAEFNLVDTQGAPATTAALRGHPTLAFFGFTHCPDVCPTTLALLANVQKQVAQPGL
                     KVALISVDPERDSPEQLGRYIASFGGDLIGLTGPAPEIVKTMKTFGVAASRVDLPGGS
                     YTMDHSAAIFAVDSGARIVAVFTPPFNAAALARDVEKLASVLSAGAGT"
     gene            15197..16090
                     /gene="prmB"
                     /locus_tag="VEW08_16065"
     CDS             15197..16090
                     /gene="prmB"
                     /locus_tag="VEW08_16065"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:TIGR03533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HYJ42310.1"
                     /translation="MARLFHITAADLIASATRRMKRARVYFGHGTDNARDEAVALVLY
                     VMKLPPDAPVRVLTRRVSAAQRERFEALLKRRLADRVPLPYLTNEAWFAGLPFYVDER
                     VLIPRSAIAELVERRFAPWISAARVTNVLDMGTGSGCIALACAKAFPRARVDGVDIDA
                     PALEVAAINRRKLRLTRRVELIESDHFTALSGRSYDIIVSNPPYVGEREMRGLPREYG
                     HEPRRALESGRDGLDSVATILHQAHQFLKPKGLLVVEVGNTERILAKAFPKLPFTWLD
                     FERGGGGVFLLTREQLEKAPS"
     gene            16090..17196
                     /gene="aroC"
                     /locus_tag="VEW08_16070"
     CDS             16090..17196
                     /gene="aroC"
                     /locus_tag="VEW08_16070"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HYJ42311.1"
                     /translation="MSGNSIGRLFTVTTFGESHGPALGCIVDGCPPGLEITEADLQGD
                     VDRRRTGTSQFVSQRREGDEVRILSGVFEGRTTGTAIGILIENTDARSRDYEKIKDRF
                     RPGHADYTYQQKYGFRDYRGGGRSSARETVMRVAAGAIARKYLHSRLGVSISGYLSQI
                     GSLAIDPVKPETAYDNPFFCPDPSRLKELEDLMWKVRGDGDSLGAKVTVMARGVPPGL
                     GEPVFDRLDADLAHAMMGINAVKAVEIGAGVASAAQSGSVHRDELTPTGFLSNNAGGI
                     LGGISSGQDVLVHVTFKPTSSIQVPGKTIDVAGNPVDVVTTGRHDPCVGLRATPICEA
                     MLAIVLMDHYLRHRGQNADVRCDTPVITDGNRAR"
     gene            17204..18229
                     /locus_tag="VEW08_16075"
     CDS             17204..18229
                     /locus_tag="VEW08_16075"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HYJ42312.1"
                     /translation="MKKTYGVAVLGATGLVGDMIIRVLQERDFPVGELFPLASTRSVG
                     KRVEFRGRHIPVIDVATFDFSRAQIGLFSAGGEVSRQYAPKAAAAGCIVVDNTSEFRY
                     EDDIPLVVPEVNPKAIGEYKKRGIIANPNCSTIQMVVALKPIHDAVGITRINVATYQS
                     VSGAGKEAVDELASQTTALMSGEEPGKPQVIAKRIAFNCVPQIDKFMDNGYTKEEMKM
                     VWETRKILGDPSILVNATAVRVPVFFGHSEAVHIETRQKITAEAARELLRRAPGIEVL
                     DERKPGGYPTAATEAANRDTVYVGRIREDISHERGLNLWIVSDNIRKGAATNSVQIAE
                     ILVREHI"
     gene            18342..21224
                     /locus_tag="VEW08_16080"
     CDS             18342..21224
                     /locus_tag="VEW08_16080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HYJ42313.1"
                     /translation="MKRMGLARVLLALLLLPGISHALGLGDVHLNSPLNAPLDAEIEL
                     VNATPEDLATLEAKLASKETFERYGLDWPQFMSTVTVTRDRSASGGQVLRIRSTETVT
                     EPFLTLLIEASWARGRLVREYTVLLDPPVFAPNSVAAAQPRPAMPSVGGAEASGTISR
                     PAQPEPQTAAPVAAGGGDSYEVQRGDSLSAIARRLSASTGARTDQLMVSIYRGNSGAF
                     EGDMNRLRAGSVLRIPSGEEIAAVSPAEASGEVRRLAGAWAGAGAASGTGRLRLVPPS
                     DSAASPGASSGNAAEVDALQGRVRELEGELTESKRLLELRNTELADLQAKLAASQQQP
                     VAAQPAPTQTPVEQPLPQPEAVTPAPDAAAPTPSAAAPEPAPTPAETPATEPAQPTPS
                     ETAAPAEGEEGPSLIDSLLGYWWVLAAGLLVVLGLLFVKSRTKRKTAEFDDSLGRLAD
                     HGEDRLAQPMDSLSDTGRMRAPNMGAIPEDILVEESGTHRALQETQDIPLAPTVRTDE
                     TISSETAINLDQGDPLSEADFHMAYGLYDQAADLVRIAIQREPDRRDLKLKLLEVFFV
                     WGNKEQFLTMARELSDSRSESGMGEWEKIVIMGKQIAPEDSMFSDGGAGSSVGVDLNL
                     DAGGSGRVDFDPFDVSARMDVTGGGNADAVDLDLGNALRDPDATGEGLMLPNNPASGQ
                     TTREMTVKMTQAGSESPTVEQPALRPLDEPTIREKVEGAMRRKLSSDQTAELALDDLG
                     LELGSLEQTDSQIGMKPVGGMPRGPDSNAPTMVAGLDENSQRLLAAAAARHGMTSGDD
                     SQLTEHGASGTWFLTERELGGDVDLTKGRALDPNSTASMAKFEMPEAGFDISSTSRLA
                     AIDRENLDFPIEPTQQQPVGGGRGKVDLDIGNGFDMPGSPTEELAVPDLEPVTLSEVG
                     TKLDLARAYVDMGDPDGARNILNEVLSEGSASQKQEAQRLLESLPG"
     gene            21250..22038
                     /gene="truA"
                     /locus_tag="VEW08_16085"
     CDS             21250..22038
                     /gene="truA"
                     /locus_tag="VEW08_16085"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HYJ42314.1"
                     /translation="MPRFAAGLEYDGRAYSGWQFQRGLQTLQDVVQRALSRVADTPVD
                     CVCAGRTDAGVHAAGQVIHFDSAAVRTERGWRLGANSYLPPDVSVVWVREVPEQFHAR
                     FSATARSYRYLILNRDSRSALAAGRATWERRPLDAARMHAAAQALVGEHDFSAFRAVE
                     CQSRSPVRRVECLEVMRSAEWVTLAITANAFLHHMVRNVAGLLMSVGHGESPPERVAT
                     VLANRDRRTNAATAPPDGLYLAAVRYPAEFGLPSQSAIIGRTFE"
     gene            22054..22938
                     /gene="accD"
                     /locus_tag="VEW08_16090"
     CDS             22054..22938
                     /gene="accD"
                     /locus_tag="VEW08_16090"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HYJ42315.1"
                     /translation="MSWFEKIMPSRIKTERRTRSVPEGLWIKCSACDAVLYRAELERN
                     LNVCPKCSHHMRIGSRERLARFLDEGEQMEIGAEVSPEDPLKFRDSKRYRDRLAAAQK
                     ATGERDALVAVAGSLEGLKIVACAFEFKFLGGSMGSVVGERFKRAVEHCIQNKVPLVC
                     FSSSGGARMQEALFSLMQMAKTSAALARLAQARMPFISVLTDPTTGGVSASLAMLGDL
                     NVAEPRALIGFAGPRVIQQTVRETLPEGFQRSEFLLEHGALDMIVDRRDMRERLGSLL
                     RMLMRQPAATPAAPVPAE"
     gene            22949..24223
                     /gene="folC"
                     /locus_tag="VEW08_16095"
     CDS             22949..24223
                     /gene="folC"
                     /locus_tag="VEW08_16095"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF008101.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HYJ42316.1"
                     /translation="MRSLAEWLEQQQRSHPNTIDLTLSRVREVARRLDLLTPDYRVIT
                     VGGTNGKGSTVACLDAMLRAAGQRCGRFTSPHLARYNERICVNGIEADDSHLIAAFER
                     IDAARAEITLTFFEYNALAALDVFRRSPVDVAVLEVGLGGRFDATNIIDADVAVVCSI
                     GIDHIDWLGDTLEAIGREKAGIFRKGRPAVLGSADLPGSVWAAIEEVGARAVVPGRDY
                     SFREGAHADRSGWSFEYGEFSLRDLPPSALAGIHQTANAAAALAALTALGGIELTHAN
                     VSQALRAVRLPGRFQRVPGDVEWIFDVAHNVPAALGLAANLRTLPRVPTIAVCGILGD
                     KDLQGITAALSADIDAWVLVTLDGPRAVSTRELARHLPADARILAHAADVAAGCRVAR
                     DAARTGDRILVFGSFLTVGPALEFLGPEFIGL"
     gene            24232..24900
                     /locus_tag="VEW08_16100"
     CDS             24232..24900
                     /locus_tag="VEW08_16100"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYJ42317.1"
                     /translation="MDSRAKQRLTGAVILVTLFVLLVPALLTGPPNSDASGTAAEDGM
                     RRYTIDLNAANPSATPVESQAAPPVALPQPASPGPTDPNPDDRPLARPGDSAEPAAAV
                     PVPSAASPAPTVTPSAVTKAQPPAQVPIPRDAAQKPAPATPVEPGSFAVQLGSFRDRD
                     NADRLVRDMTGKGFAAFVAPIKSGGRELYRVRVGPTRDRTQAEALAAQLRRVGQSGSI
                     VPIS"
     gene            25013..25519
                     /locus_tag="VEW08_16105"
     CDS             25013..25519
                     /locus_tag="VEW08_16105"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HYJ42318.1"
                     /translation="MIAVDYIIIAILIISAVMGLVRGLLREAIAVITWFLAIVLAWIF
                     ASSLEPLLGGILVGSPMRIWVARVIIFVGVLLLGGAVSVVLGHYVRVSMFAGMDKFLG
                     FVFGIIRGIVIVGAFTIAVQAMRMDEDPRWKNSRLMPYAIGVADALRGIVGEQLERID
                     LAKPSVST"
     gene            25533..>26043
                     /locus_tag="VEW08_16110"
     CDS             25533..>26043
                     /locus_tag="VEW08_16110"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060388.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HYJ42319.1"
                     /translation="MCGIAGIVGTSGVNQRIYDALTVLQHRGQDAAGIATVAGGELFT
                     RKGRGLVRDVFEQRHMEELKGNIGMGHVRYPTAGCDSAAEAQPFYVNAPYGICLAHNG
                     NLTNARELGEVLEREDRRHLNTSSDSEVLLNVLASELQRVGTPRVTPADIFAAVNAVY
                     RRCRGGYAVV"
BASE COUNT         4846 a         8328 c         8397 g         4472 t
ORIGIN      
        1 cgcaatcgat gcgagtgatt cgctcgagtt ggatacggtc gacaagaagg tttactggga
       61 cgtcgtcgac cactgttacc tctgtgacat gtgtttcatg acgaagtgtc cgtacgtccc
      121 gccacatccc tggaacatcg acttcccgca cctgatgctg cgcgccaagc acgtgcagca
      181 tcgcaagggc gagcactcct tccgcgacaa actactgtcg aacacccgcg gactcggcag
      241 catcgccggc attcccgtgg ttgccgagat cgtcaacgcg gtgaacggca ccagggtggg
      301 ccgcaagttg ttagaggcca cgctcggcgt cgatgccacg gcgccgatcc cgaagtttca
      361 ttccaacacc ggcactaagc gggcacgccg cgagtccggc aaggcgactg aggtgacgcc
      421 caccgaggaa acgcgcggca aggtcgcatt gttcgtcacc tgttacggca accgcaacga
      481 gccgcacatg cccgaagacc tggccgtcgt cttcgagcac aacggtattc cggtgaaggt
      541 ggtgggcaag gagaaatgct gcggcatgcc caagctcgag ctcggcgatc tcgagacggt
      601 cgagaagtac aagaacttca acatccctca gctcaaggcc ctgatcgatg acggctacga
      661 catcgtcgca gcgatcccct cctgcgtgct catgttcaag caggagctgc ctctgatgtt
      721 ccccgacgag gcggacgtgc agatggtcaa ggaacacatc ttcgatcctt tcgaatacct
      781 gatgttgcgc cacaaggccg gcctgctgcg cacggatttc accgccaggc tcggcaaggt
      841 cagttaccac gtgccgtgtc atctgcgcgt gcagaacatc ggcctcaaga cccgggatgt
      901 gctcaggctg gtgccggaaa ccacggtcga tgtcatcgaa cggtgttcgg gtcacaacgg
      961 cacctacgcc gtgaagaaag agttccgcgc ggcgtcggtg aagatcggca ggccggtggt
     1021 ttcgaaagtg gagcaggcga aggccgacca ctattcgagt gactgcccga tggccggaca
     1081 ccagatcgaa agcggactgt cgcccgatgc ttcgggagct gggccgcgcg aacccgaaca
     1141 tcccatgacc ctggtgcgca aggcgtacgg tttgtaatgc cgatgcacaa attagccgag
     1201 tcggatcttc tgtccctcga acgttattcg cgcgagcggc tggattttcg tgcgcgcgtc
     1261 atggcgcaca agcgcaatcg ccaggtgaac gtgggaccca acaccatgtg gctgttcgaa
     1321 gaccgcctca ccgtgcagta ccaggtgcag gaaatgctgc gcaccgagcg aatcttcgag
     1381 tccgagggca tcgccgaaga actggcggcc tacaacccgc tgattcccga cggccgcaac
     1441 tggaaggtca cgttcctgat cgagtacacg gatccggaag tgcgccgcgt ccagctcgag
     1501 cgcctgatgg gcgtcgaaga tgcatgctgg gtgcaggttg ccggcttcca tcgcgtgttt
     1561 gcgatcgcgg atgaagacct ggaacgctcg aacgaggtga aaacctcagc cgttcatttc
     1621 ctgcgattcg agctcagcga gccaatggcc tcgggtctca agggcggtgc ggcgttgtcc
     1681 atcggtatcg accaccctaa ctatcagcac caggtctcgc ccgcggccga caatgtgcgc
     1741 gccgccctgg ccgccgacct gggctgagac ttgtccacgg ggcgcgcgcc tcagagcggc
     1801 ggcaggctgc tattttcctt ctgggcagcc gccttcttct ccctgtcgaa cagcaccgct
     1861 gcgtaggcca ggttcgcgag acccagcgcg gacgggatca gtccaaaaag cgcgccctcg
     1921 acaccgcccg cggcgtgtat ggcgaagtac aggatgattc ccagtgtcag gagcaggact
     1981 cccgaccgca gcatgcgggc aggattcgac cacttgtcat ctgcccggtc ccgttcctcg
     2041 gcaatcaccg gcaagggcac gcctttttca atggctgcca tgcgctcccg gtgcgcgtgc
     2101 tcgagcttct ggcttcggcg ctggtggtcc gcccacaacc ccagcatggc gaaggaaaga
     2161 ctgaaaacga tggcgataat cggaatccag aagacgttca tggtcaaatt ctccagaaga
     2221 gcccctacag ttgcctttga aacccgccag accccgtttc ggttgcatgc aacccactgg
     2281 acccggacgg agtatcaagg gcgtgcagcc agcggtaaat atgagcattg acgcgggcat
     2341 accgcccccg gccccgaaat tgacccctgc catggagcgc gacgccgacc tggtcgccgc
     2401 tctgcgccag ggtgatcgtc gcgcggtcct ggaggggctc atgacgcggt atcgccagaa
     2461 agtcatgaat ctggcggtgt ccatcgtgcg tgaccaggcg ctggccgagg atctgtctca
     2521 gaccgtattc gtcaaagtgt ggcaggcact acccaagttc gatggccgcg cgtcgatctc
     2581 gacctggctg tacacgatca cgcgtaatac ctgcctgacg gcggtgaccc gcgaacgccg
     2641 gctggtgccg ctcgaggatt tcggtgaagt cgccgacgac gatggagatc cgagcgtgtt
     2701 cggaaccgcc gtggcggggc aggaagatgc cagccaggtc gccgctgaat acgatgtcgc
     2761 gaagctgctc gagcagttgc cggaacctta ccggcgcgtg gtggttctgt tctatctgga
     2821 agatcgaagt tgtgaagaag tgggcgaact gctgtcaatg cccaccggaa cggtcaaggc
     2881 gctactgcac cggggtcgca agcgcctggc cgcgctcgcc ggcgaaatgg agaacgagtc
     2941 atgaaatcca atcccgaaaa cgaagcgatt ctcgagcgct cactgcgcaa gcaggtgaac
     3001 gttcgccaac tggacgattc cttcgacgcg gcggtgtggg cgcgaatcga agccgaggag
     3061 tcaagatcca tggcgcaggc gccagtgcgc gcagccacgt catcgaaggt tggccgctgg
     3121 ctattcattc tcaacgcggt gggcatcgcg tccgtcgccg tgttcctgtg cgtattcctt
     3181 ttccaatggc tcgcgggagt ccaggtcgat gcgacgtttg cgaattcctc tttccgggtc
     3241 tccgatcaga ccgtgacgat atgggcttcg ggaattgcgg gtgcggcgtt gctggccggc
     3301 ttcttctata cgccgtgggg cggccgactg cgcgacgaat tcttctagct tcgcagcggc
     3361 gggtgcgctg tcgccgcacc tcttgtcact ggtagacttc gcgccgcgcg gcactgacag
     3421 gctgcgtttc ccgaggtttg tcacgcaaga atgtcttaca acgcatccga catcgaagtc
     3481 ttatccggtc tcgaacccgt acgccgccgt ccgggcatgt acacggacac ctcccgcccg
     3541 aaccatctcg cgcacgaagt ggtcgacaat tcggtcgacg aggcgctcgg cggccatgcc
     3601 aaggaaatca acgtcatcct gcacaaggac aactccttgt cggtggcaga cgatggccgc
     3661 ggtatgccgg tggacatcca cccgaaagag aaagtgagcg gcgtcgaact cattctcacg
     3721 cgcctgcacg ctggcggaaa attcaacgac aagaactacg aagtctccgg cggcctgcat
     3781 ggcgtcggtg tgtcggtcgt gaatgccttg tcgaagcagc tcgagacctg gatcaagcgc
     3841 aacggcaagg aatacaacat cagcttcaag gacggcaaag tctcctcgaa gctcgaagtg
     3901 gtcggcaccg tcggccagcg caacaccggc accacggtgc gcttctggcc ggacccgaag
     3961 tttttcgaca gcgacaaatt ttcggtgccg cagctgcggc atgtgctcaa ggcgaaggcc
     4021 gtgttgtgcc ccggcctcaa gatcaccttc gagaacgaag ccaccaaaga gaaagacgag
     4081 tggtttttca ccggcgacct tgcggcctac ctgaaggaag agatcggcaa ggaagtcccg
     4141 ctgcccgctg agcccatctt cgcaaagaac gatcagcaga agatcaacgt ggtcgagtac
     4201 gccatcgtgt ggacgccgaa tgccgaccgc gcgatgggcg agagctacgt caacctcatc
     4261 ccgacttccg aaggaggcac gcacgtcaat ggcctgcgtt ccggtgtggc cggcgcagtg
     4321 agggaattct gcgaattccg caacctcgtg ccgcgcgggc tcaagctctc gcccgaagac
     4381 atcttcgaca aatgctgctt cgtgttgtcc atcaagatcc aggagccgca gttcgccgga
     4441 cagatcaagg agcgcctcgc gtcgcgcgat gcggcgccgt tgaccgaaag cttcgtgcac
     4501 gactcgctgt cgctgtggct caaccagcat ccggatgccg gcgagcgcat cgcgcagttc
     4561 gccatcgaga atgcgcagga gcgcaccaag gctgcccagc aggtgaagcg caagcgcgtc
     4621 accgcgggtc ccgcgctccc cgggaagtta gccgactgca cgaaccagga tccgaagcac
     4681 tccgagctgt tcctggtcga aggcgattcg gccggcggct cagccaagca ggcacgcgat
     4741 cgcgagacgc aggccatcat gccgctgcgc ggcaagatcc tgaacacctg ggaagtgtcg
     4801 gtgggcgaga tcaactcctc gcaggaagtg cacgatatct cggtggccat gggcatcgac
     4861 ccgggaacca gcgatctgtc cgggctgcgt tatcacaaga tctgcatcct cgccgacgcg
     4921 gactctgacg gcctgcacat cgccacgctg ctgtgcgcgt tgttcctgcg tcactaccgg
     4981 ccgctggtgg ccaacggcca tgtgttcgtc gcgatgccac cgctgtatcg catcgacgcg
     5041 ggcaagaaca tctactacgc gctggacgac gccgaacgcg actcgctgct caagaagatc
     5101 gaaaacgaag gcaagaccaa ggcgaagccg cacgtgcagc gattcaaggg ccttggcgaa
     5161 atgaatcctt cgcagctgcg cgagaccacc atggacccgc gcacgcgccg actcgtgcaa
     5221 ctcacgatct ccgcgaagga cgacagcgac agcatcatgg acatgatgct cgccaaaaaa
     5281 cgcgcctccg accggcgcga gtggctggaa gaaaagggca acctggccga agtgatcccc
     5341 tgaaggttgg gtaagcaatg caagacgaac tgaatttcga aggcgtggaa aaacagccac
     5401 tgcgcacctt cacggagaag gcgtacctcg actactcgat gtacgtgatc ctcgatcgcg
     5461 cgctgccggc gttgtcagac ggcctgaaac ccgttcagcg ccgcatcatc tacgcgatga
     5521 gtgagctcgg cctctcgtcc gggcaaaaac acaagaagag tgcgcgcacc gtcggcgacg
     5581 tcatcggcaa gttccatccg cacggcgact cggcctgcta cgaagcgatg gttctgatgg
     5641 cgcaggactt cagctatcgc tatccgatcg tggatgggca gggcaactgg ggctcgcccg
     5701 acgatccgaa gtccttcgcg gccatgcgat atacggaatc gaaactcact cgctacgccg
     5761 aagtgctgct gtcggagctc tcggacggca ccgtcgactg gcagccgaat ttcgacggca
     5821 cgctcgagga gccggccttc ctgccggctc gcctgccgaa cgttctgctc aatggcacgc
     5881 aaggcattgc cgtcggcatg tccaccgaca ttccgccgca caaccttcgc gagattgccc
     5941 aggcttgcat ccacctgctg gatgagccgg atgcgatgac gcgcacgttg atgaagtacg
     6001 tgaagggtcc ggatcttccg acaggcggtg agatcgtctc gccgcgctct gaaatcctcg
     6061 acatctacaa caccggcaac ggcagcttca aggcgcgcgc cgtgtacaag caagaagacg
     6121 acaacatcgt catcagcgag ctgccgtacc aggtgtcggg caacaaggtc atcgagcaga
     6181 tcgccgcgca gatgcgcgag aaaaaactgc cgatggtcga agacattcgc gacgaatcgg
     6241 atcacgaaga acgcatccgc atcgtcatcg aacccaagaa gcgaaccgac gtcgcgcagc
     6301 tcatggcgca cctgttcgca acgacggatc tcgagcgcaa ctaccgcgtg aacatcaacg
     6361 tgatcacgct cgagggcaag ccgaaggtga tgggcctgcg cgacctgctc aacgagtggc
     6421 tcgtattccg caccacgacc gtgacgcgca ggctccagtt ccggctcgac aaggtcgccg
     6481 cgcggctgca catcctcgat ggcctgctgg ccgcgtacct gaacctcgac gaagtcatcc
     6541 gcatcatccg ccgcgaggac gagcccaagc ccgtgctcat gaagcggttc aagctcaccg
     6601 agatccaggc cgaggcgatt ctcgaaacca agctgcgcca cctcgccaag ctcgaagaga
     6661 tgaagatccg cgacgagcag aagaagctcg ccgaggagcg cgaggaactc gagggcattc
     6721 tcaagtcgaa ggccaagctc aagaaactcg tcgccagcga aatcgaagcc gacgccgaaa
     6781 agtacggcga cgatcgccgt acgaagatcg tcgagcgcga ggccgcgcag gccatcgacg
     6841 agtccgagct cgtggccaac gagcccgcga ccatcgttct ctcgaagggc ggtttcgtgc
     6901 gctcggccaa gggccacgag atcgacgcgc gctcgctcac ctacaaggcc ggcgacgagt
     6961 accagcatgc cgcgaagggc cgcagcgtgc agcaggcggt gttcctggat tcgaccggcc
     7021 gggcctatag cgtcgtcgcg cactcgctgc catccgcacg cgggctcggc gaaccattgt
     7081 cgtcgcgcgt cgatccgccg gatggcgcca agtggcgcgg cgtgatgatc ggcgagccgg
     7141 aagatctgtg gctggtcgcg agcgacgccg gctatggctt taccgtgcgc ctgaaggaag
     7201 cctatgccag caagaaggct ggcaagacgc tgctcaacat tcccgacgga gcgctggtgc
     7261 tgccgccggc ccccgtgccc ggcgacgatg cgctggtcgc cgtcacctcc gagggtggca
     7321 aactactcgt gtttccggtg aaggacgtgc ccgagctcgc gcgcggcaag ggcaacaagc
     7381 tgttcgacat accggccaag aagttcgcga gccgcgaaga cttcctcacc ggcatggcgg
     7441 tggtcccgaa ggacaaatcg ctggtggtgc gctcgggtga acgcaagatg acgctctcgt
     7501 ggtccgacct caaggactac cgcggtcagc gcgcgcagcg cggctcggtg ctgccgagag
     7561 gctggcgtac cgtcgacgtg ctcgaagtcg agtgattgcg tagcgacgct atcgcgtcgc
     7621 tacgaccacc gctgcgctcg ccatcgccac tccagaactg cgattcaggc gtcgcacgga
     7681 ctttggagtc gtgaagagct cgcgtgcgcg ggcggcgagg aatgcgtacg cacccagaac
     7741 ggccggcaat acgacgcaca tggccgcgga aatctccacg aaacgcacca ggttcatcga
     7801 agcgatgtcg acgacggtcg gaagcagcgc gacgaagaac aggatggtct tgggattcgc
     7861 cagcgtgagc aggaagctcg caagaaaagc cttccagccg tgctggcgat catcggcttc
     7921 cttgactgtc acgcgttccg ccggcgcgct ccacagcttc caggcgagat acagtagata
     7981 ggtgacgccg atccacttga tcacgacgaa cgcggtggcg gcggtcttcg ccagcgccgc
     8041 gaggccggtg gcagcgatgg cgaaccagac gaggtcgccg accatgaatc cggcgatgaa
     8101 cgcgggtgtc cctttcaggc catgcgcgag gccgcgcgcg atgaccgcgg cgacgccggg
     8161 gccgggcgcc gcaacggcga gcgcgtacac cgcgcagaat gtgagaaggc cgtaaatcgt
     8221 catggtttac ctcgggtccg attctagaag ggtggaggac ttggcgtcga cctggcgcgc
     8281 ataatcgcgc ccatggccac acctcttcgc gtgacactcg tcctcctcgc ttcgatgttc
     8341 gcgccgggtg cgtacgcgca agtcgccgga tctccgccgg ctggcaagac ctgggcgctg
     8401 gcgattcatg gaggcgctgg cgtcatccag cgaggcgccc tgacaaagga taaggaggtc
     8461 gcctatcgcg ccgccatgct gaccgcaatg gatgcgggtt cgaccgtcct gcgccggggc
     8521 ggctcgagcc tcgatgcagt cgaagcggcg atccgcgtgc tcgaggatga cccgttgttc
     8581 aatgccggac gcggcgccgt tttcacggcc gagggtcgca atgagctcga cgcctcgatc
     8641 atggacgggg cgacgcgcaa ggcgggcgcg gtggccggcg tgacgcgcac gcgcaatccc
     8701 atcagcctgg cgcgtgcggt catggaaaaa tctccgcacg tgatgctggc ccgcgaaggc
     8761 gcggatcaat tcgccgtgga gcagggtctg ccgcaggtcg accccgccta tttccgcacc
     8821 gagcagcgct ggcagcagct gctcgactgg cggcgtgaca acgcgaagct gctcgatcag
     8881 acgcactcgc gcggcacggt gggtgcggtc gcgatcgatg cgagcggtca cctcgccgcg
     8941 ggcacatcca cgggtggcat gaccggcaag cgctggggca gggtcggtga ctcgccggtg
     9001 atcggcgcgg gcacctacgc ttccgacggc agttgcgcgg tgtccgccac gggctcgggt
     9061 gagtacttca ttcgcgccag cgcggcgcgc cagatctgtg atcgcatagc ctggcgtggc
     9121 gacagcgtgc agtcggccac atctgccacg atcgccgata tcggcagcat cggcggtgat
     9181 ggcggcgtga tcgccatgga cggcaagggc aacatcgcct ttgcgatgaa cacgagcggc
     9241 atgtatcgcg gctgggtgac gagcacgcaa tcgcccgcca cggcaatcta ctctggcgag
     9301 gaaggccccg cgcgctagcg ggggttgggg cgaggcttga cgggtgtggc cgccgcgggc
     9361 gaagacgaca gggtgcgcag ggtctgggta tccgtgatgg tcacgtcttc gggtgcgttc
     9421 acgaaatagc cctggatgaa ctgcacgccg agctgccaca acaccgccat ggtgttggcg
     9481 tcttcgaccc gctcggcaat ggtttcgatc ttgagtttgt gtgcggcctc gaccagcttc
     9541 ttgacgacgg tctgggtttc gaagttgcca gcgaggtcct gcaccagcgc gccgtcgatc
     9601 ttgatgaagt cgagcggcac agtgctcagt agtttgatcg gatcacgacc ggagccgaaa
     9661 cgctcgagcg cgatgcggaa gcccatcttc ttgagtgcgc cgagctgggc gaccgcggcg
     9721 ttcatgtact gctcgtagat gctctcggtg atctggaagc agattcggtc gggcttgtgc
     9781 gtgccggtca cgagctgcgt cttgagccac tcggcgaagc cggcgtcgag cacgctgtca
     9841 cgcgaaaggc gcacgaacag ggcgccgggt tttttctgcg cggcgaacga cagtgaagca
     9901 ccgaccaccc agcgatcgat gttcttgagc aggtcattgc gctcggccgc agccatgaat
     9961 tccgccggca gcacttcctt gttctggtga tcgagcatgc ggaccagcac gtcgaacatg
    10021 ttcggatcgt cgccctgcag gctggccacg ggctgttgca cgaggcggaa gcggttttcc
    10081 atgagcgcgg acttgatgtg cttgacccag atcttgtcgt acgcctgcac gcgcgtgtct
    10141 gtgtcggcct tgtccagatg aaagatctgg ttgccaccct ccttgcgtgc gcggcgtacc
    10201 gcttcgacga catcggccac gatggcgtcg atattcgagc cggcgggcgg cacggtggtg
    10261 agaccaatgg tgcagctcga gtgcacggtt ttctgcccta cggtcgaaac gctctcgctg
    10321 aagcgcgcga gcacctgttc ggcccaggct tcgacgtcgt gggtgttgcc gcgctcgagc
    10381 agcgcgagga atgacactcc ggcgaaccgg ccggcgatgt cgttgggtcc gagatgtgac
    10441 ttgagcattg ccgcgtattc gctcaacagt tgttcgctgg cacgcgcacc gatgtccttt
    10501 tcgagctcgg cgaatttatc cggccgcacc aacgccatga agcgcacgcc gcccgcgggt
    10561 ggcgcgccga tgcggacgcc aatggcatcg agcaaaggcc gtcgcaacag caggccggtg
    10621 gtcgcatcgc gtttcaccgc atcgctgagg tccgtcgtga gctgacgatc gtcgcgcttg
    10681 cgcgcgggca ccatgatgcg cacgcaaggt tcgttgtcga attctccggg cgtcagcacc
    10741 agatcgagct gcagttgcgt accgtcgccg agcagagccg tcactttcaa cgggtgatcg
    10801 gtccactttc cctggccaca ggcgacgagc gcacctttca gcgcggcatg ggagcgatct
    10861 tcgaacagat ccatgagcgg ctggccgacg accacgccgg catccgcgta accgaacagc
    10921 tcgagccagg tctggttggc atcgacgacg atgccttcct gcacctgcag gatggcatcg
    10981 ttggagcgtt gcaataccga ctgcagctgg cgccggtagt cgctcgccga ctgcaacgtg
    11041 gaattgagcg cgcgttcgag ccggaacgag cgcagctcgc gcgacaccac cgcctggacg
    11101 cgatcggtgg ccgagaggga aacgacgtca cgggcaccca tgcgcatgcc cgtggcgatg
    11161 tcgccctcgg aaatctgcgg tcgcaccagc agcatcggca cctggggggc gagctgatcg
    11221 cgcaccgtgg ctgcctgctt ggcgtcggtg tcggaaacgt aggcgcacac caggagttcg
    11281 gggttgagct gttcgagcgc ctcgccgagg tctcgcactg cgggaatcca cgtgcaatgc
    11341 accggttcgc cggccttgcg catcaggctg ttgatgaatt cggacggctc acgttgcggt
    11401 gagaaaacga tgagcggaac ggcagaattg tccgtcaatt tgactcccgg gaggtgcgcc
    11461 acgcgtgcaa cgcgctggtt caaacaagcg gggcgagttt agcacccggc cacccggggt
    11521 cccgcgccgg tgtcggaaac ccagggcggc tgtttcgtat agcagcggta cacccagctg
    11581 ggtatcatgc gcggtctttt ttgcaggccc gctagtcggg aaaacccgga aatcccatgt
    11641 ccagttacac gaccaatgaa attcgcggcg gaatgaagct attgatcgac ggcgatccgt
    11701 acgcggtaat cgacaatgag tacgtgaagc caggcaaggg tcaggccttc aaccgcatca
    11761 aggtgcgcaa tctcaagacc ggccgcaccg tcgaaaagag ctatcgctcc ggcgagtcca
    11821 tcgaggcggc cgacgtcctg gatagcgaaa tgcaatatct ctacaaggac ggcgatttct
    11881 ggacgttcat gaaccccgag aactacgagc agctccaggc cggcgaagcc gccgtgggtg
    11941 atgccgccaa gtggctcaag gacggagtgg tctgcatcat cacgctgtgg aacggtgtgc
    12001 cgctggtggt gacgccgccg ccgcacatcg acctgaaagt catcgagacc gacccgggcc
    12061 tcaagggcga caccgccacc ggtggccaga aggtcgccaa gctcgaaacc ggcgcctcgg
    12121 tccgcgtgcc gctgttcatc aacgaaggtg agctgctgcg catcgacacg cgctcgggcg
    12181 aatacgtgtc ccgcgccaag ggctagttag tccagcgatt tgaggaaaat cttcgcgagc
    12241 gcttcgaggc cgcgcgcatc cgccgcgccg aagcgcgcat gcagcgggct gtcgaggtcg
    12301 agaacgccga gcagttccgg gccgcgcaac aaaggaatga cgatctcgga attcgacgcc
    12361 gaatcacagg cgatgtgccc cgggaacgca tgcacgtctt cgacgattac cgtttcgcgg
    12421 cgcgcggccg ctgtcccgca gacccccttg cccatggcga tccgcacgca cgcgggcttg
    12481 ccctggaagg gccccagcac cagttcgcca ctcttatata agtagaagcc agcccagttg
    12541 aggccgggca gggcgtcata gatgagggcc gcggtattgg cggcattggc gatcaggtcc
    12601 cgttcccccg cgagcagcgc cgcaagatca cgggcaagtt cacgataggc ggcgtccgca
    12661 tcgctgaagt catagtgctg ggtggcatgg ctcattcagg cgcgctccgt ggggaaggcg
    12721 atcacttcat cgatgctggc ggcgttcatg gcgagcatga gcacgcgatc gaaccccagc
    12781 gcgacgccgg cacaatccgg cagccccgca tccagcgccg ccagcaggtg cgcatcgagt
    12841 tcgaacgtgg gcaggccgcg ctttttacgc gactgctgat cgaccgcgaa tcgcatgcgc
    12901 tgctcggcgc cactcgtgag ctcgtgatag ccattggcga gttccaggcc gcgatggtaa
    12961 agctcgaaac gcaaagccac gcgcgggtca tcgggatcca gtcgcgcgag cgcggcctgt
    13021 gatgccggat aacgatgcag gaacgtgagc gtggccgcgc cgagtgccgg cccgacctgc
    13081 gcgccgacga tgagatcgag cagttcgtcg cgaccggcct gcgcggcgtg ggccgcatcg
    13141 agtcccagcg actgcgcagc gcgttgcaat tcacccgagt ccgcgtcgag cggatcgaat
    13201 cccgcatgac ggcgcacggc gtcgcgatag ctcacgaatt ccacggccag tcgcgtgccg
    13261 agcagttgat tggtgaggtc cgccacttcg cgcatcaatt gttcgagtga aaacccgagc
    13321 cggtaccact cgagcatcgt gaattccggg ttgtgctgac ggccgctttc cgcgccgcga
    13381 tacacgtggc agatctggaa gatgtcgccg ctgccggcgg ccagcagccg cttcatcgcg
    13441 tattccggcg aggtgtgcag gaacagcggg tcctccgtgc cggacatctg cacgcgaacg
    13501 ctgccgagat tcacgtcgga caccggcgca tggatcatgg ccggcgtctc gacttcgagc
    13561 acctggcgcg aaaagaagaa ttcgcgtgtg gcgcgcagcg cgctggcgcg ttggcgtaac
    13621 gcgcgtgtgg tcgccgtcgg ccgccacgtg gcgtcactca ccgcgttggt caccttgtct
    13681 gttccggagg cgtggaacgc cagccggaga gcgtgccgat gcgctcgccc atgcgcagcg
    13741 tattaccggc cttgagattg ccgtcccagc gcaccgcacc gggcgggaac agcaggatca
    13801 ccgtcgagcc catgttgaag cgcgccatct cgtcgccctt ggcgagctgc gcgggcgcga
    13861 gcacctcgct cacggggagt gcagttacct cgcgaacctt gcgcggcgtg acatcaccgt
    13921 gccagacggt ggccatgctg ccgacattca acgcgcccac caggatgttt gcccagggca
    13981 tgccacccga atcgaagccg cagaacaccc gctcgttgcg cgagaacagc cccggcacca
    14041 gttgcgcggt ggtgcggttc acgctgaaga ggcgacccgg cacgtagaag gcctcgcgca
    14101 gttcacccgc gagcggcatg tggatgcgat ggtagttgta cggcgcgagg tagatagtcg
    14161 cgaacgtgcc gccggcgaac gtccgctccc aggcggagtt gccggcgagc agggcgcgca
    14221 acgaatattc gtggcccttg gcctgcagca accggtcggc gctcagctcg ccggcctcgc
    14281 tcaccgtgcc gtcgacgggc gagacgatgg cttcagggtc cgcgtccacc gggcgcgtgc
    14341 cgtcgcgaag cgcgcgcgtg aagaattcgt tgaagcttgc gtacgcggtc ggctccgttt
    14401 ggacggcatc gttcatgtcc gggttgaaac cgcgtacgaa cagccggatc agcgcattct
    14461 tgaatgcggg tgcacgtgaa cgcgttgcgg cgagcaccaa ccgggagatg ccgtgttgcg
    14521 gcagcaggtg ttgaagcgcg acgaacgcgc ggccagaggc gccgggactg ctcatgtgcc
    14581 ggctccagcc gacaacaccg acgccagttt ttcgacatcg cgtgcgagcg cggcggcatt
    14641 gaacggcggc gtgaacaccg cgacgatgcg agcgccggag tcgaccgcga aaatggcggc
    14701 cgaatgatcc atggtgtagc tgccacccgg aaggtcgacg cggctcgccg ccacgccgaa
    14761 agttttcatg gtcttcacaa tctcgggcgc cggccccgtg aggccgataa gatcgccgcc
    14821 gaatgacgcg atgtacctgc cgagctgctc cggactgtca cgttccggat ccacgctgat
    14881 gagggctacc ttgaggccgg gctgcgccac ctgtttctgc acgttcgcga gcagcgcgag
    14941 cgtcgtggga caaacgtcgg gacagtgtgt gaagccgaag aatgcgagag tagggtgccc
    15001 gcgcaacgcg gcggtcgtag ccggtgcgcc ctgcgtgtcg acgagattga actcggccag
    15061 cggccgcggc cgcgggaaca cggtgcccga ctcgagtgaa gccacagcgg gctggcccag
    15121 catgcgcgcc acgtacacgc cggccagagc ggctgccgcc gcaattgccg ccacggaaat
    15181 ccagtaacga ttcgacatgg cgcgattatt ccacatcacc gcagcggacc tgatcgcaag
    15241 cgcgacgcgg cgcatgaaac gcgcgcgcgt gtacttcggg catggcaccg acaacgctcg
    15301 cgatgaagcg gtggccctgg tgctgtacgt catgaaattg ccgccggacg ccccggttcg
    15361 agtgctgacc cgccgcgtca gcgccgcgca gcgagagcgt ttcgaggcgc tgctgaagcg
    15421 gcgtctcgcc gatcgcgtgc cgctgcccta cctcacgaac gaagcctggt tcgccggcct
    15481 gccgttctac gtcgacgaac gcgtgctgat tccgcgttcc gccatcgccg aactcgtcga
    15541 acggcgcttc gcgccgtgga tcagcgccgc gcgggtgacg aacgtgctcg acatgggtac
    15601 gggctccggc tgcattgccc tggcctgtgc gaaggcattt ccgcgtgcgc gcgtggacgg
    15661 cgtggacatc gacgcgccgg cactcgaagt cgcggccatc aatcgccgca aacttcgtct
    15721 aacacgtcgg gtggagctca tcgaaagcga tcatttcacg gcgctttcgg gcaggtccta
    15781 cgatatcatc gtgagcaatc cgccctatgt gggagagcgc gaaatgcgcg gcctgccacg
    15841 cgagtacggt cacgaaccgc gacgcgctct cgaatcaggt cgtgatggtc tcgattccgt
    15901 tgcgaccatc ctgcatcagg cgcaccagtt tctgaagccg aagggtctgc tcgtggtcga
    15961 agtcggcaac accgagcgaa tcctcgcgaa ggcatttccg aaattgccgt tcacctggct
    16021 cgatttcgag cgcggtggag gcggtgtgtt cctgctgact cgagaacagt tagagaaggc
    16081 cccaagctga tgtcgggtaa ttccataggc cgattgttca ccgtgaccac ctttggcgag
    16141 agccacgggc ccgcgctcgg ctgcatcgtc gacggctgcc cgcccggtct cgaaatcacc
    16201 gaggcggacc tgcagggcga cgtggatcgc cgccgcaccg gcacatcgca gttcgtgagc
    16261 cagcggcgcg aaggcgacga ggtcaggatt ctttccggtg tgttcgaagg caggacgacg
    16321 ggaacggcca tcggcatcct catcgagaac accgatgcgc gttcgcgcga ctacgaaaag
    16381 atcaaggacc gctttcgtcc cggccatgcg gactacacct accagcagaa atatggcttt
    16441 cgcgactacc gcggcggtgg ccgctcatcg gcgcgcgaaa ccgtcatgcg cgtcgcggcc
    16501 ggcgctatcg cgcgcaaata cctgcacagc cgcctcggtg tcagcatcag cggctacctg
    16561 agccagatcg gctcgctggc catcgacccg gtgaaacccg agactgcgta cgacaacccc
    16621 tttttctgcc cggatccgtc gcgcctgaaa gagctcgaag acctgatgtg gaaggtgcgt
    16681 ggcgacggcg attcactcgg tgcgaaagtc actgtgatgg cgcgcggcgt gccaccgggc
    16741 ctcggcgagc cggttttcga tcgcctcgac gccgatctcg cgcacgcgat gatgggcatc
    16801 aatgcggtga aagccgtcga aatcggcgcc ggtgtggcgt ccgccgcgca gtcgggcagc
    16861 gtgcatcgcg acgaactcac gccgacgggc ttcctgagca acaacgccgg cggaattctc
    16921 ggcggcattt cctcgggaca ggacgtgctc gtgcacgtca cgttcaagcc cacctcgagc
    16981 atccaggttc caggcaagac catagacgtc gccggcaacc cggtcgatgt ggtgaccacg
    17041 ggtcggcatg acccctgcgt gggcttacgt gccacgccca tctgcgaagc catgctggcg
    17101 atcgtactga tggatcatta tctgcggcac cgcggccaga acgccgatgt ccgctgcgac
    17161 acgccggtga tcaccgacgg taaccgggca aggtagttag gatatgaaga aaacgtatgg
    17221 tgtggccgtg ctcggagcca ccgggctcgt gggtgacatg atcatccgcg tgctccagga
    17281 gcgtgacttc ccggtcggcg agctgtttcc gctcgccagc acccgctcgg tcggcaagcg
    17341 tgtggagttt cgcggccggc acattccggt catcgacgtc gccaccttcg acttctcgcg
    17401 cgcgcagatc gggctgttct ccgcgggcgg cgaagtctcg cgccaatacg ctcccaaggc
    17461 ggcggccgcc ggctgcatcg tcgtcgacaa cacatccgag ttccgctacg aggacgacat
    17521 tccgctggtg gttcccgagg tgaatcccaa ggcgatcggc gagtacaaga agcggggcat
    17581 catcgccaat ccgaattgct cgaccatcca gatggtcgtg gcgctcaagc cgatccacga
    17641 tgcggtgggc atcacgcgta tcaatgtggc cacctatcaa tcggtgtcgg gcgcgggcaa
    17701 ggaagccgtg gatgaactgg cttcgcaaac gacggccctc atgagcggcg aggagccggg
    17761 aaaaccccag gtcatcgcca agcgcatcgc cttcaactgc gtcccgcaaa tcgacaaatt
    17821 catggacaac ggctacacca aggaagaaat gaagatggtg tgggagaccc ggaaaatcct
    17881 cggcgacccg tccatcctgg taaatgccac ggcggtccgt gtgccggtgt tctttggcca
    17941 ttccgaggcg gtacacatcg aaacgcggca aaagatcacc gccgaggcgg cgcgcgaact
    18001 actcaggcgc gccccgggaa tagaggtgtt agacgagcgg aagcccgggg gttaccccac
    18061 ggcagccacc gaagccgcga accgggacac ggtttatgtc ggacgcatta gggaagatat
    18121 aagtcacgaa cgcggactta acttatggat cgtgtcggac aacatccgga agggtgctgc
    18181 cacgaacagc gtccagatcg ccgagatttt ggtgcgagag catatttaag ttatattgcg
    18241 ggcggtttca tgcttacgcc ggaagtttcg gcgctaagct catgtttcga taaagaaatt
    18301 acatagatat tggatcgccg acggtggtag tggagacgcg aatgaagcgg atgggtctcg
    18361 cccgggtttt gctggcgctt ttgctgttgc ccggaatttc ccatgcactt gggctaggcg
    18421 acgttcacct gaattcgccg ctcaacgcgc cactcgacgc ggagatcgaa ctcgtcaacg
    18481 cgacgccaga agatctggcg acgctcgaag cgaagctcgc gtcaaaagaa acgtttgaac
    18541 gttacggcct cgactggccg cagttcatgt ccaccgtgac cgtcacgcgc gatcgctcgg
    18601 cgagcggcgg gcaggttctt cgcattcgct ccaccgagac ggtcactgaa cctttcctca
    18661 cgttgctcat cgaggcctcg tgggcccgcg gtcgcctggt gcgcgaatac acggttctgc
    18721 tcgatccgcc ggtgtttgcg cctaacagtg tcgcagcggc gcagccgcga cccgccatgc
    18781 cctccgtggg tggcgccgaa gcttcgggca cgatttcccg tcccgcgcag cccgagccgc
    18841 agactgctgc ccccgtcgca gctggcggtg gcgacagtta cgaagtgcag cgtggtgaca
    18901 gcctctcggc catcgcccgc cgcctgtccg cgagcaccgg tgcgcgcacc gaccagctca
    18961 tggtgtctat ctaccgtggc aactccggcg ctttcgaagg cgacatgaat cgcctgcgcg
    19021 cgggttccgt gctccgtatt ccgagcggtg aagaaatcgc cgcagtctcg cccgcagaag
    19081 ccagcggcga agtgcgtcgc ctcgcaggtg cctgggccgg agcaggtgcg gcgtcgggta
    19141 ccggccgcct gcgcctggtg ccgccgagtg actcggccgc aagtcccgga gcgtcatccg
    19201 gcaacgccgc agaggtcgat gccctccagg gccgcgtgcg cgagctcgaa ggcgaactca
    19261 ccgaatccaa gcgtttgctc gagctgcgca ataccgaact tgccgatctg caggccaagt
    19321 tggcggcctc gcagcagcag ccggtggccg cgcagcctgc gcccacgcag acgccagtag
    19381 aacagccatt gccgcagcca gaggcggtta cgcccgcgcc ggatgccgcc gcgccaacgc
    19441 cgagcgctgc cgcacccgaa ccggcaccga cgccggcaga aacgcctgcc acggagcccg
    19501 cgcaacccac tccgtccgag acggccgcgc ccgcagaagg tgaagaaggg ccgtcgctga
    19561 tcgactcgtt gctcggatac tggtgggtcc ttgccgccgg cctgctggtc gtcctgggcc
    19621 tgctgttcgt caagtcgcgg accaagcgca agactgccga attcgacgac agcctcggcc
    19681 gccttgccga tcacggcgaa gaccgtctcg cccagccgat ggattcgctc tccgataccg
    19741 gccgcatgcg cgcgccgaac atgggcgcga ttcccgagga catcctcgtc gaagaatcgg
    19801 gcacgcaccg cgcgctgcag gaaacccagg acatcccgct cgcgccgacg gttcgtaccg
    19861 acgaaaccat cagcagcgaa accgccatca atctcgacca gggcgatccg ctgtcggaag
    19921 ccgatttcca catggcctac ggcctgtacg accaggcggc cgatctggtg cgcatcgcga
    19981 ttcagcgcga gcccgatcgt cgcgacctca agctcaagct cctcgaagtg ttcttcgtct
    20041 ggggcaacaa agagcagttc ctcaccatgg cccgtgagct ctcggactcc cgttcggaat
    20101 ccggcatggg cgagtgggaa aagatcgtca tcatgggcaa gcagattgcc cccgaagatt
    20161 cgatgttctc cgacggtggc gccggcagct cggtgggtgt cgacctcaat ctggatgccg
    20221 gtggttcggg ccgtgtcgac ttcgatcctt tcgatgtcag cgcccgtatg gacgttaccg
    20281 gtggcggcaa tgccgacgcc gtcgatctcg atctcggcaa cgcgctgcgt gatccggacg
    20341 ccacgggcga aggcctgatg ctgccgaaca accccgcgag tggtcagacg actcgcgaga
    20401 tgaccgtcaa aatgacccag gccggctcgg aatcgccgac ggtggaacag cccgcactgc
    20461 gtccgctcga tgagccgacg attcgtgaga aagtggaagg cgccatgcgt cgcaagctga
    20521 gctcggatca gaccgccgaa ctcgctctcg acgacctcgg cctcgaactc ggttcgctgg
    20581 agcagacgga ttcgcagatc ggcatgaagc ccgtgggcgg catgccccgc ggacccgact
    20641 ccaatgcacc cacgatggtc gcgggcctcg atgaaaactc acagcgtctg ctggcggccg
    20701 cggctgcgcg ccatggcatg accagcggcg acgattcaca gctcaccgaa catggcgcca
    20761 gcggcacctg gttcctcacc gagcgtgagc tgggcggcga cgttgacctg accaagggcc
    20821 gtgccctcga cccgaacagc actgcttcga tggcgaagtt cgaaatgccg gaagccggct
    20881 tcgatatttc ctcgacgtcg cgcctggcgg ccatcgatcg cgaaaacctc gatttcccga
    20941 tcgaaccgac gcagcagcag ccggtcggtg gcggacgcgg caaagtggac ctcgatatcg
    21001 gcaatggctt cgacatgccg ggcagcccga ccgaagaact cgccgttccg gatctcgagc
    21061 cggtgacgct ctcggaagtc ggcaccaagc tcgacctggc tcgcgcctac gtcgacatgg
    21121 gtgacccgga cggtgcgcgc aacattctca acgaagtgct gtcggaaggc tccgcttcgc
    21181 agaagcagga agcgcagcgc ttgttagagt cgctgcccgg ctagtccgcg cggggcttca
    21241 gagacctgga tgcctcgatt cgcggccggc ctcgagtacg acggccgggc gtactccggc
    21301 tggcaattcc agcgcgggct tcaaaccttg caggacgtgg tgcagcgagc gttatcgcgc
    21361 gtggctgata cgcccgttga ctgcgtgtgc gccgggcgca ccgacgcggg tgtgcacgcc
    21421 gcggggcagg tgatccattt cgacagcgcg gcagtgcgca ccgaacgcgg ctggcggctc
    21481 ggcgccaaca gctatctacc gcccgacgtg agcgtcgtct gggtgcgtga agtacccgag
    21541 cagttccacg cgcgcttcag cgcgaccgcg cgcagctacc gctacctcat cctcaatcgc
    21601 gattcgcggt cggccctcgc ggcgggcagg gcgacctggg agcggcgtcc gctcgatgcc
    21661 gcacgcatgc acgcggccgc gcaggcgctg gtgggtgaac atgacttcag cgcgttccgc
    21721 gcggtggaat gccagtccag atccccggtg cggcgcgtgg agtgtctcga ggtgatgcgc
    21781 agcgccgagt gggtcacgct cgccatcacg gccaatgctt tcctgcatca catggtgcgt
    21841 aacgtcgcgg gcctgctgat gtcggtgggg cacggagaat caccgccgga acgcgtggcc
    21901 acggtgctcg ccaaccgcga tcgaaggacc aatgcggcga ccgcgccacc cgatggcctg
    21961 tatctcgccg ccgtgcggta tccggccgag ttcggtctcc cgtcgcaatc cgctatcatc
    22021 ggccgcactt tcgaataggg gtcgcccacc gacatgtctt ggttcgagaa aatcatgcct
    22081 tcgcggatca agaccgagcg ccgcacgcgt tcggtacccg agggattgtg gatcaagtgt
    22141 tcggcctgcg acgcggtgct gtaccgcgcc gagctcgagc gcaatctcaa tgtctgtccg
    22201 aagtgcagcc accacatgcg catcggcagc cgcgaacggc tggcgcgttt cctcgacgaa
    22261 ggcgaacaga tggagatcgg cgccgaagtg tcgcctgagg atccgctcaa gtttcgcgac
    22321 agcaagcgtt atcgcgatcg tctcgcggcg gcgcagaagg ccaccggcga gcgagatgcg
    22381 ctcgtcgcgg tcgccggttc gctcgaagga ctcaagatcg ttgcctgcgc gttcgagttc
    22441 aagttcctcg gcgggtccat gggctcggtg gtaggcgagc ggttcaagcg cgcggtggaa
    22501 cattgcatcc agaacaaggt gccgctggtg tgcttctcgt cgagtggcgg cgcgcgcatg
    22561 caggaagcgc tcttttcgct gatgcagatg gccaagacca gcgcggccct ggcgcggctg
    22621 gcgcaggccc gaatgccgtt catttccgtg ctgactgatc cgaccaccgg tggtgtttcg
    22681 gcaagccttg cgatgctcgg cgatctgaac gtggccgagc cgcgtgcact gatcggcttt
    22741 gccggtccgc gtgtcatcca gcagaccgtg cgcgaaacac tgccggaagg gtttcaacgc
    22801 agcgaattcc tgctcgagca cggcgcgctc gacatgatcg tcgatcgccg cgacatgcgt
    22861 gagcgcctcg gttcgctgtt gcgcatgctc atgcggcagc cagccgccac gcccgcggcg
    22921 cccgtccctg cagagtaggg tcaccggcat gcgctcgctc gccgagtggc tcgagcagca
    22981 gcagcggtca caccccaaca ccatcgacct caccttgagc cgggtgcgcg aggtggcgcg
    23041 acgtctcgat ctgctcacgc cggactaccg tgtcatcacc gtcggtggca ccaacggcaa
    23101 gggttcgacg gtcgcctgcc tcgatgcgat gttgcgcgca gctggccagc gctgcggtcg
    23161 cttcacctcg ccgcatctcg cacgttacaa cgagcgcatc tgcgtgaatg gcatcgaggc
    23221 tgacgactcc cacctcatcg ccgccttcga gcgcatcgac gcggcgcgcg cggaaatcac
    23281 gctcacgttc ttcgaataca acgcgctcgc ggcgctggac gttttccgcc gttcgcctgt
    23341 tgacgtggcc gtgctcgagg tggggctggg cggacgattc gacgccacca acatcatcga
    23401 cgcggacgtc gccgtggttt gctcgatcgg catcgaccac atcgactggc tgggagacac
    23461 gctggaagcc atcggcaggg aaaaggccgg catctttcgc aagggccgtc ccgcggtcct
    23521 cggcagcgca gatctgcccg gcagtgtgtg ggccgccatc gaagaggttg gcgcgcgtgc
    23581 cgtggtgccg ggtcgcgact acagttttcg cgagggagct cacgcagaca ggagcggatg
    23641 gagtttcgag tacggggagt tttcgctgcg cgatctgccg ccgtctgcgc tcgcggggat
    23701 acaccagacg gccaatgcgg ccgctgcgct tgccgctttg actgccttgg gtgggatcga
    23761 gctcacgcat gccaatgtca gccaggcgtt gcgggcggtg cggcttccag ggcgcttcca
    23821 gcgcgtgccc ggcgatgtcg agtggatctt cgatgtggca cacaacgttc cggccgcact
    23881 cggtctcgcc gcgaatctgc gcacgctgcc gcgcgtgccc acgatcgcgg tgtgcggaat
    23941 tctcggcgac aaggacctgc aggggatcac ggcggcgttg agcgctgaca tcgacgcgtg
    24001 ggtgttggtg acgctcgacg gtccgcgcgc cgtgtcgacg cgcgagctcg cacggcacct
    24061 gccggccgat gcccgcatcc tggcgcacgc ggccgatgtg gctgccggat gccgcgtggc
    24121 tcgcgacgct gcgcgaaccg gcgatcgcat cctcgtgttc ggatcattcc tcacggtggg
    24181 gccggcgctc gaattccttg gcccggaatt cattgggcta tagttcgcca catggattcg
    24241 cgagccaagc aacgtctgac cggcgccgtc atcctcgtta cgctgttcgt gctgctggtc
    24301 cccgcactgc tgaccggtcc gcccaactcg gatgcgtcgg gtaccgcggc cgaagacggc
    24361 atgcgccgct acacgatcga cctcaacgca gccaacccgt cggcaacgcc ggtggaatct
    24421 caggccgcac cgccggtcgc cttgccgcag ccggcgagcc cgggtccgac ggacccaaat
    24481 ccggacgaca ggccgctcgc caggcccggg gactcggccg agcccgcagc ggcagtgcct
    24541 gtgccttccg cagcgtctcc agctccgact gtgactccat ctgccgtgac gaaagcgcag
    24601 cccccggcac aggtgccgat tccgcgcgac gcagcgcaga agccggcgcc ggcgaccccc
    24661 gttgaacccg gcagcttcgc cgtgcagctt ggaagttttc gcgatcgcga caacgcggat
    24721 cgcctcgtcc gcgatatgac cggcaagggt ttcgctgcct tcgtcgcccc aatcaagagt
    24781 ggggggcgcg agctttaccg ggttcgagta gggccaaccc gtgaccggac tcaggcggag
    24841 gccctggccg cgcaactgcg gcgagtgggg cagtccggca gcatcgtgcc aatttcgtga
    24901 cgcctgccag cgtctgccaa acacttggct gctttagccg ccggccgtct tgtacaatgc
    24961 cgcgcccgcc atacgggcgt cgcggcctga ttcaccgaac cagacggacc gaatgatcgc
    25021 agtcgactac atcatcatcg cgattctgat tatttccgcc gtcatgggcc tggtacgagg
    25081 cctgctccgc gaagccatcg cagtcatcac ctggtttctt gccatcgtgc tcgcatggat
    25141 tttcgcgtcc tcgctcgagc ccctgctggg cggcatcctc gtcggttccc cgatgcgtat
    25201 ctgggtggcg cgggtcatca tcttcgtcgg cgtcctcctg ctgggcggcg cggtgagcgt
    25261 ggtacttggt cattacgttc gcgtctccat gtttgccggc atggacaagt tcctggggtt
    25321 cgtcttcggc atcatccgcg gcatcgtcat cgtcggcgcc tttaccatcg ccgtgcaggc
    25381 gatgcgcatg gatgaagacc cgcgttggaa gaactcccgg ctgatgccct acgccattgg
    25441 cgtcgccgat gccctgcgcg gcatcgttgg cgagcaactc gaaagaatcg atctggccaa
    25501 accctccgtc tccacctgag gatctgaacc gaatgtgcgg catagccgga atcgtcggaa
    25561 ccagcggagt caatcaacgg atctatgacg ccctgacggt gctccagcat cgcgggcagg
    25621 atgccgcggg catcgccacc gtcgccggcg gcgaactgtt tactcgcaag gggcgaggtc
    25681 tcgtccgcga cgtcttcgag cagcgccaca tggaggagct caagggcaac atcggcatgg
    25741 ggcacgttcg gtacccgacc gccggctgcg acagcgccgc ggaagcccag cccttctacg
    25801 tgaacgcacc ctacggcatc tgtctcgcac acaacggcaa tctcaccaat gcccgtgagc
    25861 tcggcgaagt actcgagcgc gaagaccgcc ggcacctcaa caccagctcc gattccgaag
    25921 tgctgctcaa cgtgctggcc tcggaacttc agcgcgtggg tacgccgcgc gtgacacccg
    25981 ccgacatttt cgccgcggtg aacgcggtgt atcgccgctg ccgcggaggc tacgcggtgg
    26041 tcg
//
LOCUS       DASQJS010000408        10293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1518169, whole genome shotgun sequence.
ACCESSION   DASQJS010000408 DASQJS010000000
VERSION     DASQJS010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518169"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="VEW08_16115"
     CDS             <1..1690
                     /locus_tag="VEW08_16115"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HYJ42320.1"
                     /translation="ITAVSGSRAQGWSAQGRSEVLARHGIVATSDPLAAQAGLEILQK
                     GGNAIDAAVATGAVLDVTSPNDTGIGGDLFALVYIARDKKLYALNSAGWAPAQWTPEY
                     FTQKLGVKAMPPGGVNATTVPGAISGYDALLKRFGTMTFKQTFDRAARIAEEGWGQAE
                     RRHADLLRVVPALRADADSKAAFLIGEDAPPLYGIIRNRPLGAALRLLQSQGRDAFYK
                     GEIAAAIVEKIKANGGVMSAADLAEFQSEWVEPVSTNYHGFDVFELPPPGQGFAALEM
                     LNILEVCVPKLGRNLAALGPTDPMYWHLMVEAKKLAYADLLAKNADPKFVDVPVAQLL
                     SKAHAASRCERINLEVASSTTEPVKPDGGTIYLTTADRWGNMVSLVHSVYSVYGSKAT
                     VGKFGFALQNRGAGFSLDPKSPNVVAPRKRPFHTIIAGFVMRDGKPLMTFGNMGGSVQ
                     PETHAQHMVNLIDLGMNVQMTTDAARFTHRQTDNVLGLEDNLFALVGAALKAKGHNVE
                     AVNGSAVGGYQGILFTADPILRKTEPKSGKEELPIDGVYRAGSDHRKDGQAVGW"
     gene            complement(1712..2482)
                     /locus_tag="VEW08_16120"
     CDS             complement(1712..2482)
                     /locus_tag="VEW08_16120"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HYJ42321.1"
                     /translation="MTNTRRPERIVMVTAALAFAASAAVTVAWCSSMSAMPGMDMPGG
                     WKMSMAWMRMPGQSWTGAVGTFLGMWIVMMIAMMLPVLVPMLMRYRRAVDATHLARLT
                     IVVGVAYFLVWAAFGLAVYPVGVALAESAMTQPSLARATPIFIGLVVTISGLLQFSSW
                     KARQLACCRANPVQGLRPDSRAAFRHGTQLGILCIRCCLGPTAVLLCLGVMDLRAMAI
                     VTAAIALERLSPDGLRFARLTGTFATTAGLWLTVQAAM"
     gene            complement(2460..3224)
                     /locus_tag="VEW08_16125"
     CDS             complement(2460..3224)
                     /locus_tag="VEW08_16125"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HYJ42322.1"
                     /translation="MTHITGTRQEWLDARLELLKAEKELTRRSDALAEQRQAMPWVRI
                     DKEYRFETDSGTASLKDLFRGRSQLMVYHFMFGPDYKAGCPSCSMIADGFEGFAVHLA
                     NHDVALCAVSRAPLAKLQAYKRRMGWTFPWASSAGGEFNFDFNIAFTEAEQRAGTIEY
                     NYARGGHAMDASAKWPEPLVQFAATCGTDAPTFTRDRPGMSTFVLEDGVVYHAYSTYA
                     RGLDGLWGAYQWLDRAPLGRNESGVWWKRHDEYAKA"
     gene            3313..4533
                     /locus_tag="VEW08_16130"
     CDS             3313..4533
                     /locus_tag="VEW08_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42323.1"
                     /translation="MDALITAAGRALAAGDPLGALKLVSLRDDAPALALRGIAMAQLG
                     EFARARVLLRGAARGFGAKAPVARARCVVAEAEVALASRDLGWRANALAAARATLETH
                     GDLANAAHARYLVVRRLLLIGRLVDAERALAELDAAKLTPALRAAHELVVAGIAIRRI
                     RTKDARAALMRAERAARDAGIPALWAEVDVALRILNTMAARRIARGRDEPLRLEEVEA
                     LLASKAFVVDACRLTVRQSGIVIPLAKRPVLFTLARALAEAWPNDVSRDALITRAFRL
                     KRADESLRARLRVEIGRLRRVLRPLADITATKQGFALGIRRAREVVVLALPVEEEYAQ
                     VLALLADGESWSSSALSLVLGTSQRTVQRALDDLAAAGKVQTFGRGRARRWLTPPLPG
                     FTTALLLPTSLAID"
     gene            4543..5190
                     /locus_tag="VEW08_16135"
     CDS             4543..5190
                     /locus_tag="VEW08_16135"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HYJ42324.1"
                     /translation="MTNTKADIVREYGPFDGVDGVHGVTYDGENVWFCSGDRLNAVDP
                     DSGKVKRTLEIASNAGTAFDGKHLFQIAADKINKIDPATGRVLHSIPAPGSGGDSGMA
                     WAEGSLWVGEYRARKIHQVDPETGKVLRTIESNRFVTGVTWVDGELWHGTWENEASEL
                     RHIDPDTGTVLDSVEMPAGVFVSGLESDGKDRFYCGGGKSGKVRAVRRPKRGHSR"
     gene            5264..6553
                     /locus_tag="VEW08_16140"
     CDS             5264..6553
                     /locus_tag="VEW08_16140"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HYJ42325.1"
                     /translation="MNNLSKLPAVVLGSAWLLGSSSGVPAVENRSVYSANHGTTLPGT
                     LRKSEGQAPNSDADVEAAYTSTGAFYEGFYNFWGRDSYDGLGARITSTVHYSDNYCNA
                     FWNGLQMVFGDGNGIDCLSYAASVDNTAHELTHAITARESGLIYSGESGGINESMSDI
                     FGAFVEAWVDGGKSGVLVTSDDTWKHGEDIIAPALRYLNDPAADGASADFYTSGTGNL
                     DVHYSSGVGNLAFYLLSQGGVHPRGKSPIQVQGIGMEKAIRIFYEANVNILTASSNYR
                     ALRDAAVEASITLSYDRDTSRSVVCAFAAVGVVGGIGARVCESMLANGTPLVGLADEQ
                     VGHFQHWNIDVSAGQTLTVTLAGGSGNADLYVRAGARPNVNAFDCRPRLRGNTETCSI
                     VADADGIYWIGVRTQAAYADVSLLATLSTLNAPIALR"
     gene            complement(6946..7188)
                     /locus_tag="VEW08_16145"
     CDS             complement(6946..7188)
                     /locus_tag="VEW08_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42326.1"
                     /translation="MKSILDGDKPSDIFKALLRDKPTLTNGDLALEFRKAFPSTGVDG
                     MSVIWKWKTPGTKVGLSDEALDNQLRYWLTQYGYLK"
     gene            complement(7196..8362)
                     /locus_tag="VEW08_16150"
     CDS             complement(7196..8362)
                     /locus_tag="VEW08_16150"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HYJ42327.1"
                     /translation="MTNMQPLRIALLAGCLLCSSLASPQDAKAPEPAVVAPPPRPTPP
                     TRAPDIPGAPVFTKVTGKGANAPIDKSGDFLIGPDYAPAPELEPKAVVPQGEVWQFVM
                     HSMDSKFYPGIAREVFGTVDPNNPRTLIVETRAKPYQRTITVYVPTQYKRGTKAPFIV
                     THDGPALDKSDTVLPPILDTLIAHGRIPPLIAVMIQNGGGDAQGSERGLEYDTMSGKF
                     AEFIEAEVLPAVEQNTKVQLTRNAKGRAVMGCSSGAAAALSMAWYHPEWYSKVISYSG
                     TYVNQQWPFNPETPGGAWGYHDGIIANAKPKPIRIWMHVGDRDLYNPNVMRDDMHDWV
                     VANHRMAKVLKDKGYDYQYVYALDSGHCDRKVREQTLPQALEWVWRGYPKKNSR"
     gene            8413..9144
                     /locus_tag="VEW08_16155"
     CDS             8413..9144
                     /locus_tag="VEW08_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HYJ42328.1"
                     /translation="MQSGNPVLNTKTFETVPVGERMTIGGTINKTAMLLALVMITAIY
                     TWGQFTATKDPATIMPLLWTGVIGGLIFCLITSFKSEWAPITAPIYALLEGLVIGGLS
                     AFFEMAYPGIVVQAAGLTFGTLFVMLMAYKSGLIQATEKFKLGVIAATGGIALLYVVS
                     LVMSMFGKPIGFIHEGGTFGILFSGFVVVIAALNLVLDFDFIRQGSEQGAPKYMEWYG
                     AFALIVTLVWLYLEILRLLAKLRKE"
     gene            9151..9714
                     /locus_tag="VEW08_16160"
     CDS             9151..9714
                     /locus_tag="VEW08_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42329.1"
                     /translation="MCYSAQIQAAYMKYLRETGAEMDIDQFVEIFGARVSDSSIRIPR
                     AVERWFDEPKTDGERKIKGFIDEYRAAEITKLEREVFTQKKRLADAERTLASKPTKAA
                     AESQRIAGNKIEQAMTRLDRMKAAKPHVAEARIFPMHYAPIVLQDGGRRVMRLGRYHC
                     RKPGEPEFIDRKLPGLYNAFGAFLTLM"
     gene            complement(9833..>10293)
                     /locus_tag="VEW08_16165"
     CDS             complement(9833..>10293)
                     /locus_tag="VEW08_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42330.1"
                     /translation="ALFNHPGLRRRLKEIIALSLVALCVTGCAFTPSRLNADPTKFNG
                     LTVIARGYVDLSRESHALYDSRVRFLDMQRQRERGGGEVNGEAYCISIAKPEFFYKNY
                     ALLNGRVITLKGKFTIDRLEGNSVDHRSCLTSIVVDTADLARRYQLRATR"
BASE COUNT         1916 a         3160 c         3297 g         1920 t
ORIGIN      
        1 catcacggct gtgtccgggt cgcgcgccca aggctggtcg gcgcagggcc gatcggaagt
       61 tctcgctcgc cacggcatcg tcgcgaccag cgacccgctc gctgcgcaag cgggcctcga
      121 gatcctgcag aagggcggca acgccatcga tgcggcggtg gcgaccggcg ccgtgctcga
      181 tgtcacgtcg cccaatgaca ccgggatcgg cggcgacctc tttgcgctgg tgtacatcgc
      241 cagggacaag aagctctacg cgctcaattc cgcgggctgg gcgccggcgc aatggacgcc
      301 cgaatacttc acgcagaagt taggcgtcaa ggcgatgccg ccgggcggtg tcaatgccac
      361 gacggtgccg ggcgcgattt cgggttacga cgcgttgctc aaacgcttcg gcacgatgac
      421 cttcaagcag acgttcgatc gcgccgcgcg catcgccgag gaaggctggg gccaggcgga
      481 gcgacgccat gcggatctgt tgcgcgtcgt gccagcgctg cgagcggatg cggattcgaa
      541 ggcggcattc ctgatcggtg aggacgcgcc accgctctac ggcatcattc gcaaccggcc
      601 cctgggcgcg gcgttgcggt tgctgcagtc gcagggcagg gacgcgttct acaagggcga
      661 gatcgccgct gccatcgtgg agaagatcaa ggcgaatggc ggcgtgatgt ccgccgccga
      721 tctcgccgag ttccagtcgg agtgggtcga gccggtctcg accaactacc acggcttcga
      781 tgtgttcgag ctgccgccgc cgggtcaggg attcgcggcg ctcgagatgc tgaacatcct
      841 cgaagtgtgt gtgccgaagc tcggcaggaa tctggcggcg ctcgggccga cggatccgat
      901 gtactggcac ttgatggtcg aggcgaagaa gctcgcgtac gcggacttgc tcgcgaagaa
      961 cgcggatccg aaattcgtgg acgttccggt ggcgcagctt ctgtcgaaag cgcacgcggc
     1021 gtcgcggtgc gaacgcatca acctcgaggt tgcatcgtcc acgaccgaac cggtgaagcc
     1081 cgatggcggc accatctacc tgacgacggc cgaccgctgg ggcaacatgg tgtcgctggt
     1141 gcacagcgtg tacagcgtct acggcagcaa ggcgaccgtg ggaaaattcg gtttcgcgct
     1201 gcagaaccgc ggcgccggtt tttcactgga tccgaagagc cccaatgtcg tggcgccgcg
     1261 caagcgcccg ttccatacca tcatcgccgg gttcgtgatg agagacggaa agccgctgat
     1321 gacattcgga aacatgggcg gctcggtgca gcccgagacg catgcgcagc acatggtgaa
     1381 tctcatcgac ctcggcatga acgtgcagat gacgaccgac gccgcgcgct tcacgcaccg
     1441 ccagaccgac aatgtgcttg ggctcgaaga caatctgttc gcgctggtgg gtgcagcact
     1501 caaggcgaag ggccacaacg tggaggcggt gaacggctcc gctgtcggcg gttaccaggg
     1561 cattcttttc acagcggacc cgatactgcg aaagacggag ccaaagtccg gcaaagagga
     1621 gctgcccatc gacggcgtgt accgcgccgg ctcggatcac cggaaagacg ggcaggcggt
     1681 gggctggtga gcgcctctta gcgttggcga tctacatggc ggcctgaacc gtcagccaga
     1741 ggcccgccgt ggtggcgaaa gtgccggtga gccgtgcgaa cctcaggcca tcgggtgaga
     1801 gccgttccag cgcgatcgcg gcggtgacga tcgccatcgc gcgaaggtcc atgaccccga
     1861 ggcaaaggag gaccgccgtc ggcccgagac agcatcggat gcagaggatg ccgagctgcg
     1921 ttccgtggcg gaacgccgct ctcgagtccg gccgcagtcc ctgtaccgga ttcgcgcggc
     1981 agcaagccag ctggcgcgct ttccacgagg agaactggag caggccggaa atcgtcacca
     2041 ccaggccgat gaagatcggg gttgcacggg ccagcgacgg ctgtgtcatc gcggattcgg
     2101 cgagcgcaac gcccactgga tagaccgcga gaccgaacgc ggcccagacg aggaagtacg
     2161 ccacgcccac gacgatggtt agtcgcgcga gatgagtggc gtccactgcc cgccggtaac
     2221 gcatcagcat gggcacgagc accggcagca tcatcgcgat catcatcact atccacatgc
     2281 cgagaaacgt acccacggcg ccggtccagc tctgtcccgg catccgcatc caggccatcg
     2341 acatcttcca gccgcccggc atgtccatgc ccggcatcgc cgacatcgac gagcaccatg
     2401 caaccgtcac tgcggcgctg gccgcgaagg ccagcgccgc agtgaccatg acgatgcgct
     2461 caggccttcg cgtattcgtc atgacgcttc caccagacgc cactctcatt gcggccgagt
     2521 ggcgcgcggt ccagccactg gtacgcgccc cagaggccat cgagcccgcg cgcataggtg
     2581 gaatacgcgt gatagacgac accatcttcg agcacgaacg tgctcatgcc cgggcggtct
     2641 cgcgtgaagg tgggcgcatc agtcccgcag gttgccgcga actgcacgag aggctcaggc
     2701 cacttggcgc ttgcatccat cgcatggccg ccacgcgcat agttgtattc gatggtgccg
     2761 gcgcgttgct ccgcttcggt gaacgcgatg ttgaagtcga agttgaactc gccgccggcc
     2821 gaggaggccc agggaaatgt ccagcccatg cggcgcttgt aagcctgcag ctttgccaac
     2881 ggcgcacgcg aaaccgcgca caacgcaacg tcgtggttgg ccaggtgaac cgcaaaacct
     2941 tcgaagccgt cggcgatcat cgagcacgaa gggcagcccg ccttgtagtc ggggccaaac
     3001 atgaagtgat agaccatgag ctgcgagcgg ccgcggaaca ggtctttgag cgaggccgtt
     3061 ccggaatcgg tctcaaacct gtactcctta tctatacgta cccagggcat ggcctggcgt
     3121 tgctcggcca gtgcgtcgct gcgtcgggtg agttccttct cggccttcag cagctcgagc
     3181 cgggcgtcaa gccattcctg ccgtgtgcca gtgatgtgtg tcattgcggt tctctcttgt
     3241 gatggcggtt catgggataa atttagagcc gggcagcgac acagcgggag tgacaagttt
     3301 ggcgggattc ttttggatgc actgatcacc gcggcagggc gcgcgctcgc ggccggcgat
     3361 ccgttgggcg cgttgaaact ggtttcactg cgcgatgacg cacccgcgct tgcactgcgc
     3421 ggcatcgcga tggcccagct cggtgaattc gcgcgcgcca gggtgttgtt gcgaggagcg
     3481 gcgcgtggct tcggtgccaa agcgcccgtg gcgcgggcgc gctgcgtggt ggccgaagcg
     3541 gaggtggcgc tggcttcgcg cgatctcgga tggcgcgcga atgctctcgc agcggcccgc
     3601 gcgacgctcg agactcatgg cgatctcgcg aacgccgcgc atgcgcgtta cctcgtagtg
     3661 cgccggctgc ttctcatcgg ccggctggtc gatgccgagc gcgctctcgc cgaactcgat
     3721 gcggcgaaac tcacgccggc gttgcgcgcc gcgcatgaac tcgtcgtcgc gggcattgcg
     3781 atacgccgca ttcgcacgaa ggacgcgcgt gccgcgctga tgcgcgccga gcgcgcggca
     3841 cgcgatgccg ggattccggc gctctgggcg gaagtggacg tggcgttgcg cattctgaat
     3901 accatggctg cgcgacgcat cgccaggggc agggacgaac ccttgcggct cgaagaagtg
     3961 gaagcactgc tggcatcgaa ggcattcgtg gtcgatgcct gccggcttac cgtgcgccag
     4021 tcgggcatcg tgattccgct cgcgaagcgc ccggtgttgt tcacgctggc gcgagcgctg
     4081 gcggaggcat ggccaaacga tgtatcccgt gatgcgctca tcacgcgcgc attccggctg
     4141 aagcgcgccg acgagtcgct gcgcgcgcgg ctgcgcgtgg aaatcgggcg attgcgccgc
     4201 gtgctccggc ccctggccga tatcactgcc accaaacaag gtttcgcgct cgggattcgg
     4261 cgtgcgcgcg aggtggtggt gcttgcgctg ccggtagaag aagaatatgc gcaggtgctc
     4321 gcgcttctgg ccgatggcga atcctggtcc agctccgcgc tgagtctcgt gctcggcacc
     4381 agccagcgca ccgtgcagcg cgcactcgac gatcttgccg ccgccggcaa agtgcagact
     4441 ttcgggcgcg gccgcgcacg ccgctggctg acgccgccgc tgcccggatt cacgacggcc
     4501 ttgttactcc ccacttcgct ggcgattgac taggattcgc gcatgacaaa caccaaagct
     4561 gacattgttc gagagtacgg gccgttcgac ggcgtcgacg gcgtgcatgg cgtcacttac
     4621 gacggcgaga acgtgtggtt ctgctcaggc gaccggttga acgccgtcga tcccgacagc
     4681 ggcaaggtga agcgcaccct cgaaatcgcc tcgaatgccg gaaccgcatt cgacggcaag
     4741 catttgtttc agatcgccgc cgacaagatc aacaagatcg atccggcgac cggccgtgtg
     4801 ctccattcca ttcccgcgcc aggctccggc ggagattccg gaatggcgtg ggccgaaggg
     4861 tcgctgtggg tgggtgaata ccgcgcgcgg aaaatccacc aggtcgatcc ggagaccggc
     4921 aaggtactgc gcacgatcga atcgaaccgc ttcgtcaccg gcgtcacctg ggtagatgga
     4981 gagctgtggc atggcacgtg ggaaaacgag gccagcgagc tgcggcacat cgatccggac
     5041 accggcaccg tgctggacag cgtggagatg cccgcgggag tcttcgtgtc cggcctggaa
     5101 tccgatggca aggatcggtt ctattgcggc ggcggaaaga gcgggaaggt gagggcagtg
     5161 cgtcgaccca agagaggcca ttcgcgctag cggcgatccc cgtacagccg gctccatgag
     5221 tggatgtccg gtttagctgc ccgtttacgt tcctcacagt gaaatgaaca atttatccaa
     5281 actacccgcg gtggtgctgg gttcagcgtg gttgcttggg tcatccagcg gcgtccccgc
     5341 cgtggagaac cgctcggtgt actcggcgaa ccatggcacc accttgcccg gcacattgcg
     5401 caagtcagaa gggcaagcgc cgaattcaga tgcggatgtc gaagccgcgt atacgagcac
     5461 cggcgcgttc tacgaagggt tctacaactt ctggggtcgc gactcctacg acggcctcgg
     5521 agcccggatc accagcaccg ttcactacag cgataactac tgcaacgcat tctggaatgg
     5581 attgcagatg gtgtttggcg acggcaatgg catcgattgc ctgtcgtacg cggcgagcgt
     5641 ggataacacc gcgcacgagc tcacgcacgc aattacggcg cgagagtccg gactcatcta
     5701 ctcgggcgaa tccggcggaa tcaacgagtc catgtccgac atcttcggcg cgttcgtgga
     5761 agcctgggtt gatggcggga agtcgggtgt gctggttacg tcggatgaca cctggaagca
     5821 tggcgaggac atcattgcgc cggcgttgcg ttacctgaac gacccggctg ccgacggcgc
     5881 atcagccgat ttctacacca gcggcactgg aaatctcgac gtccactaca gctcgggcgt
     5941 gggcaacctg gccttctact tgctgtcgca gggcggagtg catccgcgcg gcaagagtcc
     6001 gattcaggtc caaggcatcg gcatggagaa agcgattcgc atcttctacg aagccaacgt
     6061 gaatatcttg accgccagtt ccaactaccg cgcgctgcgc gatgcggccg tagaggcgag
     6121 catcacactg agttatgacc gcgacaccag cagatcggtc gtctgtgcat tcgctgccgt
     6181 gggtgtcgtc ggcgggattg gcgccagggt gtgtgaatcc atgctcgcga acggaacacc
     6241 tctcgtcggt ctcgccgacg agcaggtggg ccactttcag cactggaaca tcgacgtttc
     6301 ggccgggcaa accctgacgg tgacgcttgc cggcggcagc ggcaacgcgg atctttatgt
     6361 gcgcgcgggg gccaggccca acgtgaatgc gttcgattgc aggccacggc tgcggggcaa
     6421 cacagaaaca tgcagcatcg ttgcggacgc ggacggcatc tactggattg gcgtccgaac
     6481 gcaggcggcg tatgcggatg tttcattgct agctaccctc agcacgctga atgcgccaat
     6541 tgccctaagg taacggctga cgccaactac catccctcgt atgagggagc gaactaactc
     6601 cgaatgggtc tcagaaatga gggtggggtg ccgctaccat gcacgcccgt cgccacccgg
     6661 cgatttttac ccattggagt atttcaaatg tcttcgtcga atcttgctgt gcgcatggag
     6721 cccacggcaa cggacctgct ctctcacccg ccgcgtccct tgcttcgtgt tgtcacgaac
     6781 gaggatacgt cagaggtgcg cagcatccgc ccccgtacta cgctccagag ccgaaagatc
     6841 gtgacccgcg atcagggctt caggtttttc aatcaccgtt gatttttcag tctagctccg
     6901 ccatcgatcg catctgcgac gatgcgcgga tgcgatgacc ccggctcact tcagatagcc
     6961 gtactgggtc aaccaatacc tgagttgatt gtcgagtgcc tcatcgctca ggcccacctt
     7021 cgtgcctggc gttttccatt tccagatgac ggacattccg tcgacgccgg ttgatgggaa
     7081 cgcttttcgg aactcgagcg cgagatcgcc gttggtcaac gtcggcttgt cgcgcagcaa
     7141 cgccttgaag atgtcgctcg gcttgtctcc gtcgaggata cttttcatcc gcaatctatc
     7201 gtgaattctt cttcggatac ccgcgccaca cccactccag tgcctgcggc agcgtctgct
     7261 cgcgcacctt cctgtcgcaa tggccggagt cgagcgcata gacgtactgg tagtcgtagc
     7321 ctttgtcctt gagcaccttc gccatgcggt ggttagccac cacccagtcg tgcatgtcgt
     7381 cgcgcatgac gttcgggttg tatagatccc ggtcccccac gtgcatccag atgcggatgg
     7441 gtttcggttt ggcattggcg atgatgccgt cgtgataacc ccaggcgccg cccggcgtct
     7501 ccgggttgaa cggccactgc tggttcacgt aggtgcccga atagctgatc actttggaat
     7561 accactccgg gtgataccag gccatcgaga gggcggccgc cgcgcccgaa ctgcaaccca
     7621 tgaccgcgcg gcccttggcg ttgcgcgtga gctgcacttt cgtgttctgc tccaccgccg
     7681 gcagcacttc cgcctcgatg aactccgcga acttgcccga catcgtgtcg tactcgagac
     7741 cgcgctcgct gccctgggcg tcgccgccgc cgttctggat catcaccgcg atcaggggcg
     7801 gtatgcgccc gtgcgcgatg agggtgtcga ggattggtgg caacacggtg tccgacttgt
     7861 ccaatgccgg tccgtcatgc gtgacgatga acggcgcctt cgtgccgcgc ttgtattgcg
     7921 tcgggacgta gaccgtgatg gtccgctgat aaggcttcgc gcgcgtctcg acgatgagcg
     7981 tcctgggatt gttggggtcg accgtgccga atacctcgcg cgcaatgccg gggtagaact
     8041 tgctgtccat ggaatgcatc acgaactgcc agacctcgcc ctgcggaacg accgccttgg
     8101 gttcgagttc gggtgcaggg gcgtagtcgg ggccgatgag gaaatcacca ctcttatcta
     8161 taggagcgtt cgcgcctttg cctgtcacct tggtgaagac gggggcgcca gggatgtcgg
     8221 gagcgcgcgt ggggggagtg gggcgcggcg gcggcgcaac cacggctggc tctggcgcct
     8281 tggcgtcttg agggctggca agcgagctgc acagcaggca gccggctaac agggcgatcc
     8341 gcaacggttg catgttcgtc atggtgaggg atggtaaggt attccgcgct ccgacaacgt
     8401 ccgggaggac aaatgcaatc tggaaatccc gttctgaaca ccaagacctt cgagaccgtc
     8461 cccgtcggcg agcgcatgac catcggtggc accatcaaca agaccgccat gctgctggcg
     8521 ctggtcatga tcacggccat ctatacctgg ggccagttca ctgcgaccaa ggatcccgcc
     8581 accatcatgc cgttgctctg gaccggcgtt atcggcggcc tcatcttctg cctgatcacc
     8641 tcattcaaga gcgagtgggc ccccatcacc gcgcccatct acgcgctgct cgagggtctc
     8701 gtgatcggtg gcttgtccgc gttcttcgaa atggcgtacc cgggcatcgt cgtccaggcc
     8761 gccgggctca ccttcggaac attgttcgtc atgctgatgg cgtacaagtc cgggctcatt
     8821 caggccacgg aaaagttcaa gctcggcgtg atcgcggcca ccgggggcat cgcgctgctg
     8881 tacgtcgtca gcttggtgat gagcatgttc gggaaaccta tcggcttcat ccatgagggc
     8941 ggcacgttcg gcattctgtt cagcggattc gtggtggtca tcgccgcgct gaatctggtg
     9001 ctggatttcg acttcatcag acagggttcg gagcagggcg cgcccaagta catggagtgg
     9061 tatggcgctt tcgcgctcat cgtgacgctg gtctggctgt atctcgaaat cctgcggttg
     9121 ctcgcgaagc tgcgcaaaga gtaagcgcgc atgtgctact cggcgcagat ccaggccgcg
     9181 tacatgaaat atctccgcga gaccggcgct gagatggaca tcgaccagtt cgtcgagatc
     9241 ttcggcgcgc gcgtcagcga ttcgagcatt cgcattccgc gcgccgtcga gcgatggttc
     9301 gacgaaccca agaccgatgg cgagcggaag atcaagggat tcattgacga ataccgggcc
     9361 gccgagatca cgaagctcga gcgcgaagta ttcacgcaga agaagcgtct cgccgatgcc
     9421 gagcgcacgc tcgcatccaa gccgaccaag gccgcggccg agagccagcg cattgccggc
     9481 aacaagatcg agcaggcgat gacgcggctc gatcgcatga aagccgcgaa gccgcatgtc
     9541 gccgaggcgc gcatctttcc catgcattac gcgcctatcg tcctgcagga cggtggccgg
     9601 cgcgtcatgc ggctcggccg ctatcactgc cgcaagccgg gcgaacccga gttcatcgat
     9661 cgaaaactac ccggcctgta caacgctttc ggtgcgttcc taacacttat gtgattctgc
     9721 aggtcgccgc cacttatgcc actgaagagt tcaccagagc cgttcaactc caactcaggc
     9781 acgcgcgctc gcaattcttg agagcctcag tccggaaaag caggaggcgg cctcatctgg
     9841 tagcgcgcaa ttgatatcgt cgcgccaggt cggcagtgtc gacaactatt gaagtcaaac
     9901 atgacctgtg atcaacagaa ttgccttcta gtctgtcgat agtgaatttg cctttcaacg
     9961 tgataacccg gccgttcaaa agcgcgtagt tcttgtagaa aaactccggc ttcgcgattg
    10021 aaatgcaata agcctcgcca ttcacctcgc caccgccgcg ctctcgctgt ctctgcatat
    10081 ccaaaaaacg tacccgtgag tcgtagagag cgtgcgactc ccgagaaaga tcgacataac
    10141 ctcgtgcgat gacggtcagg ccgttgaact tcgttggatc agcgttcagc cgacttgggg
    10201 tgaatgcgca ccctgtgacg catagtgcaa ctaagctcag cgcgatgatc tctttcaacc
    10261 tccgtcgcag cccaggatga ttgaataaag cgc
//
LOCUS       DASQJS010000409         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_893291, whole genome shotgun sequence.
ACCESSION   DASQJS010000409 DASQJS010000000
VERSION     DASQJS010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893291"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1497
                     /gene="miaB"
                     /locus_tag="VEW08_16170"
     CDS             124..1497
                     /gene="miaB"
                     /locus_tag="VEW08_16170"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HYJ42331.1"
                     /translation="MPGRYYIKTFGCQMNEYDSTRMGDVLEAGANLTPTNDPAEADVL
                     LMNTCSVREKAEDKVYSLLGEWRALKKLKPHVLIGVGGCVASQEGEGITKRAPYVDLV
                     FGPQTLHRLPDMIGQKQRTGRAVVDVSFPEIEKFDHLPAPRAEGASAYVSIMEGCSKY
                     CTFCVVPYTRGDEVSRTFDSVMDEVRSLASQGVGEITLLGQNVNAFAGAMQDGTVVDL
                     ATLIHYVAEVDGVQRIRFTTSHPVEFGDSLIEAYANVPKLANQLHLAVQSGSDRILSA
                     MKRGYTALEFKEKLRRLRAVRPTISVSTDIIVGFPGETDRDFEATLKLVREANFDQSY
                     TFIYSQRPGTPAASLPDDVSAEMKQERLARLQAQLNEQARAISNQMVGSTQRVLVARP
                     SKKDSAELAGRTENGRWVNFAGPASLIGHFVDVVIVEARPQSLRGRFVSATATTIPAG
                     VQRLAAN"
     gene            1497..2477
                     /locus_tag="VEW08_16175"
     CDS             1497..2477
                     /locus_tag="VEW08_16175"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HYJ42332.1"
                     /translation="MATPAPIEPVREFDLVPPDNTRLANLVGPLDENLRLIEDRMAVT
                     VKRRGANFRITGARSHAAEDVVKDLFEATRHEDVSLERVHLALRDSRDMPGSLGVHEP
                     DDGGSVRVLRGTIRARGKNQRDYLANIHSRDLSFGIGPAGTGKTYLAVACAVEALQAD
                     KIRRIILVRPAVEAGERLGFLPGDLTQKVDPYLRPMYDALYEMLGFDRVAKYIERNVI
                     EVAPLAFMRGRSLNDSFIILDEAQNTTPEQMKMFLTRIGFGSRAVVTGDVTQTDLPAG
                     KMSGLRHVINILKNTEGVAFTFFEAADVVRHPLVQRIVKAYEAHTPPDVL"
     gene            2546..2950
                     /gene="ybeY"
                     /locus_tag="VEW08_16180"
     CDS             2546..2950
                     /gene="ybeY"
                     /locus_tag="VEW08_16180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HYJ42333.1"
                     /translation="MNSWAAAALGRRGAGREIAVRVVAASESRALNKLWRGKDKPTNV
                     LSFPAPVQSKRGMPRDMTLPLGDLVICAQVVRQEAVRDGKPVEAHWAHMIVHGSLHLA
                     GFDHEVGLRQRLRMERREISVLKRFGIGNPYL"
BASE COUNT          578 a          934 c          957 g          528 t
ORIGIN      
        1 agagggcgct tgtcggggtt tcgagggccc tcggggcgac gttaaaccct tgtttttaat
       61 tggaaatatg gcgggcgggg taagtgcgat accctagcgc ccgctttccc tgaccctttc
      121 tcgatgcccg gccgctatta catcaagact ttcggctgcc agatgaacga gtacgactcg
      181 acccgcatgg gcgatgtcct cgaagctggc gcgaatctca ccccgacgaa cgacccggcc
      241 gaggccgacg tcctgctcat gaacacctgc tcggtgcgtg agaaggccga ggacaaggtg
      301 tattcgctgc tgggtgaatg gcgcgcgctc aagaagctca agccgcacgt gctcatcggc
      361 gtgggtggct gcgtggcgag ccaggaaggc gagggcatca ccaagcgcgc gccgtacgtc
      421 gacctcgtgt tcggaccgca gaccctgcat cgcctgcccg acatgatcgg ccagaagcag
      481 cgcaccgggc gcgccgtggt cgatgtgtcc ttcccggaaa tcgagaaatt cgaccacctg
      541 ccggcgccgc gcgccgaagg cgcgagcgcc tacgtttcga tcatggaagg ctgcagcaag
      601 tactgcacgt tctgcgtggt gccgtacacg cgcggcgacg aagtgtcgcg gactttcgat
      661 tcggtgatgg acgaggtgcg ctcgctggcc tcgcagggcg tgggcgaaat caccttgtta
      721 ggccagaacg tcaacgcctt tgcgggcgcg atgcaagacg gcacggtggt cgaccttgcg
      781 acgctcatcc actatgtggc cgaggtggac ggcgtacaac gcatccgctt cactacttcg
      841 caccccgtgg agttcggcga cagcctcatc gaggcgtatg ccaacgtgcc gaaactggcc
      901 aaccagcttc atcttgcggt gcagtcggga tcggatcgca tcctcagcgc gatgaagcgc
      961 ggctacacgg cgctcgaatt caaggaaaag ctgcgccggc tgcgggccgt gcgtcccacg
     1021 atatcggtgt ccactgacat catcgttgga tttccaggcg agacggatcg cgacttcgag
     1081 gccacactga aactcgtgcg cgaagccaat ttcgaccagt cgtatacgtt catttacagc
     1141 cagcgcccgg gcacacccgc agcctcgctg ccggatgatg tcagcgcaga aatgaagcag
     1201 gagcgcctcg cgcggctgca ggcgcagctc aatgaacagg cgcgtgccat cagcaaccag
     1261 atggtcggca gcacgcagcg cgtgctggtc gcgcgtccgt cgaaaaagga ttccgcggaa
     1321 ctcgcgggcc gtaccgagaa cgggcgctgg gtgaacttcg ccggccccgc gagtctcatc
     1381 ggtcatttcg tcgacgtggt catcgtcgaa gcgcggccgc aatcgttgcg cggccgtttc
     1441 gtgagcgcca cggctacgac gattcccgcg ggcgtgcaac gcctcgccgc aaattgatgg
     1501 ctactcccgc tcccattgaa ccggtgcgcg aattcgatct cgtgccgcca gacaatacgc
     1561 ggctcgccaa tctcgtcgga ccgctcgatg aaaacctgcg cctgatcgaa gaccgcatgg
     1621 cggtgactgt gaagcggcgc ggcgcgaact tccgcatcac gggtgcgcgt tcacacgcag
     1681 ccgaagatgt ggtgaaagat ctgttcgaag cgacacgtca cgaagacgtc tcgctcgagc
     1741 gcgtgcacct ggccctgcgc gattcgcgcg acatgccggg ttcgctcggc gtgcacgaac
     1801 ccgacgacgg tggttccgtg cgcgtccttc gcggcacgat ccgcgcgcgc ggcaagaacc
     1861 agcgcgacta tctcgcgaat atccacagca gggacctgtc tttcggcatc gggcctgcgg
     1921 gcactggcaa gacgtacctg gccgtggcct gtgcggtcga ggccctgcag gccgacaaga
     1981 tccgccgcat catcctcgtg agaccggcgg tcgaagcggg tgaacgcctg ggattcctgc
     2041 ccggcgatct cactcagaag gtggatccgt atctgcgtcc gatgtacgac gcgctctacg
     2101 agatgctcgg cttcgatcgc gtggccaagt acatcgagcg caatgtcatc gaagtggcgc
     2161 cgctcgcgtt catgcgcggc cgctcgctca acgactcatt catcattctc gacgaggcgc
     2221 agaacaccac gcccgagcag atgaagatgt tccttacgcg catcggcttc ggctcgcgtg
     2281 cggtggtcac gggcgacgtc acgcagacgg acttgcccgc cgggaagatg tccggcctgc
     2341 ggcacgtgat caacatcctg aaaaataccg aaggtgtggc gttcacattc ttcgaggcgg
     2401 ccgacgtggt tcgtcatccg ctcgtgcagc gaatcgtcaa ggcgtatgaa gcgcatacgc
     2461 cgcccgatgt cctatgagtg aagcgcgagc gttgaaactg gatctcgctc gcggcacacg
     2521 tgcctggacg cccgcgcgtt ctcgaatgaa cagttgggcg gcagctgccc tgggccgacg
     2581 aggtgcaggc cgtgagatcg cagtgcgtgt cgtcgcagct agcgagagtc gcgcgctgaa
     2641 caagctctgg cgcggcaagg ataaacccac gaacgttttg tcgtttcctg cgccagttca
     2701 atcgaagcgc ggcatgccca gagatatgac actgccactg ggtgacctcg tcatctgcgc
     2761 gcaggtcgtg cgccaggaag ccgtgcgtga cggcaagccg gtcgaggcgc attgggcaca
     2821 catgatcgtg cacggctcac tgcacctggc aggcttcgac cacgaagtcg gcctccgcca
     2881 acggctgcgc atggagcgcc gcgagatttc cgtgctgaaa cgattcggga tcgggaatcc
     2941 ctacctgtga gcgccaagaa caaagagaag gaaaaggaaa aggaacgcga catcggc
//
LOCUS       DASQJS010000410        11007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4346260, whole genome shotgun sequence.
ACCESSION   DASQJS010000410 DASQJS010000000
VERSION     DASQJS010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11007
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4346260"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..523)
                     /locus_tag="VEW08_16185"
     CDS             complement(59..523)
                     /locus_tag="VEW08_16185"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYJ42334.1"
                     /translation="MTLGVGKKVPVFSLPLSDGSLWKSTDARGGKLVLYFYPKDMTPG
                     CTLEGQDFRDLHAAFRRTKTTVLGISKDSCARHEKFRAKEKFPFELLSDEGETVCKLF
                     DVIREKSLYGRKFMGIERSTFLIDENGKLAREWRKVRVKGHAAEVLEAAKAL"
     gene            complement(520..1050)
                     /locus_tag="VEW08_16190"
     CDS             complement(520..1050)
                     /locus_tag="VEW08_16190"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HYJ42335.1"
                     /translation="MKQHLAVSAIGSDRTGMVHDLTRVITDCGGSISESRMASLGNEF
                     AMLVLVSGNWHSLAKLESELTRLGEASGLSLHMKRTEPRAPRTELLPYSIDVVCLDQA
                     GIVTGLSGFCASRGIDIAEVSTRTYPAAHTGAQMFAVQMIINVPKRLQLAHLREEFMD
                     YCDAQNLDAILEPVKS"
     gene            1143..2027
                     /gene="dapA"
                     /locus_tag="VEW08_16195"
     CDS             1143..2027
                     /gene="dapA"
                     /locus_tag="VEW08_16195"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003317384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HYJ42336.1"
                     /translation="MFAGSLVALVTPMLPDGSIDFDAWSRLLEFHVANGTAGIVVAGS
                     TGESATVTDGELRDLLVHARRVLGKRAWVIANAGTSSTASSVERARWISELGIDALLI
                     ASPAYVRPTQQGLFLHFEAIAANSRIPVLLYNVPSRTACDILPSTVARLSEVPRIVGI
                     KEAIGDGARVRELVASCAAEFKVLSGDDLTARDAIGLGASGVISVTANAAPRAMADMV
                     AAALRGDRAGATQIDQRLVALHRNLFVESNPIPVKWAVARMGLMGGTLRLPLTELSEE
                     FHAVVTESLFAAGITVAR"
     gene            2059..2343
                     /locus_tag="VEW08_16200"
     CDS             2059..2343
                     /locus_tag="VEW08_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42337.1"
                     /translation="MRIPTLLIACCLAVLVTGCKSHGTTCKQDNKDYVGAEEMPPLKA
                     PPGLEPPDTRNALKVPPLDTPERPRSKDEACLDVPPPFTNPKGPDAAKPK"
     gene            2420..2509
                     /locus_tag="VEW08_16205"
     tRNA            2420..2509
                     /locus_tag="VEW08_16205"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2454..2456,aa:Ser,seq:gga)
     gene            2531..2627
                     /gene="ffs"
                     /locus_tag="VEW08_16210"
     ncRNA           2531..2627
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VEW08_16210"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2670..4253
                     /gene="dnaX"
                     /locus_tag="VEW08_16215"
     CDS             2670..4253
                     /gene="dnaX"
                     /locus_tag="VEW08_16215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HYJ42338.1"
                     /translation="MSYLALARKWRPRSFAELVGQEHVRQALGNALTQGRVHHAFLFT
                     GTRGVGKTTIARILSKCLNCDTGVTATPCGTCASCKEIDEGRFVDLIEVDAASRTKVD
                     DTRELLENVQYSPTRGRYKVYLVDEVHMLSTHSFNALLKTLEEPPPHVKFLLATTDPQ
                     KLPVTVLSRCLQFNLKRLPVSLISEHMNKVLTAEKIAFDAPALRLVAQAADGSMRDAL
                     SLLDQLIAFGGGKVEEASARGMLGTIDRDHVTRLARALATGDAAQLLETARSLEEFSP
                     DYGQVLDDLAGLLTRAALQQLVPGYEGDELFDPMLLKELAAAIPAEDMQLYYQTAILG
                     RRDLPFAPDPRTGFEMTLVRMLAFRPAGGAAVASTPGGGGARVAASAPGGALPSTTSA
                     AVVRPAAAGPIDPVQWSRVIGDLDVSGAARQLATNCALVEHRGKTLKLAVDSKVARTP
                     PQVEKLAQALAKYLGGHVVLEFVDGAPPVETPAQTGERRSAEALDMARRVLEEDPTVR
                     EMKSRFGATLHPESVRPTE"
     gene            4265..4585
                     /locus_tag="VEW08_16220"
     CDS             4265..4585
                     /locus_tag="VEW08_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019826710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HYJ42339.1"
                     /translation="MRGGGNMMKQAMALQANMKKAQDEIANMEVIGESGGGMVKVTMS
                     GKHEVKRVQIEPQVASEDREMLEDLIAAAVNDAVNKVEQQSQARMSSVMSGVALPPGF
                     KMPF"
     gene            4588..5184
                     /gene="recR"
                     /locus_tag="VEW08_16225"
     CDS             4588..5184
                     /gene="recR"
                     /locus_tag="VEW08_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003254304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HYJ42340.1"
                     /translation="MQHAAPLAKLIDALRALPGVGPKTAQRMAFHLLQGGRGGARALA
                     DALSAGLASIKRCQRCRMLTDTDLCSICAAPARDAALLCVVESPADVVAVEQSGSYRG
                     RYFVLHGHLSPLDGIGPEQIGAREFEALLDSGEVQEVILATNPTVEGETTAHFLGERA
                     AQRAIRASRIAHGVPIGGELEYVDGGTLAHALTGRISV"
     gene            5503..8259
                     /locus_tag="VEW08_16230"
     CDS             5503..8259
                     /locus_tag="VEW08_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYJ42341.1"
                     /translation="MSKFSESMQRWLGNKSISRVIMILIGLAVTPALVVTVSMVIREI
                     AQIKFLHEEYRAIVEFHPLEEITSHGTNRMILGQMPEGQRDAAKYAEFDAELTLAVEE
                     VKAAVAGAGKPALQPFWDAVEAQYEKVHAMAPQSVSPDEWFRTHEELAELTLKLRDRV
                     GVETGVVLDPGAETLPLVDAMFNHISSLETSVMRAAGHSFVAAGGTMSAEASDGLATA
                     ATHIDELTAEIHMDFDDAILASADGEEGYKSVVADRDATLNGMKVLAEKVRQMRASGT
                     AADHAAMISAALTVLGAIDELHDSGSPKLEALLRDRESRTRWMIALVASFLLLFISVS
                     VWLGVLASRGVSRSLGIAVSTAENIAAGRYDNTIEAQGENETGRVLRAVGAMQAELKR
                     RTENESLAREKERAVAAESMRVKVGLDSVSGNVLLADKDNRIIYLNKAIQQMFRAAAA
                     DFRRQRPNFDAEALIGTPMDLHGSGAGATRHAHQTQVKIGSRTFRVSTSPVVDDGGLW
                     LGTVAEWIDRTQEVAVEEEIANIVVAASAGDLSMRIRKEGKDGFFATLADGMNGILTN
                     MSAVVAEVNSVIEAGKNQDLTARIDIAGKSGVFEQLSGGINALMDSMMSVVQQIKSAA
                     QSVASGSDEIAKGNADLSGRTEQQASSLEETASSMEEMTSTVKHNADNASQANQLARA
                     ARQEAEQGGAVVSNAVAAMQGINASSKKIADILGVIDEIAFQTNLLALNAAVEAARAG
                     EQGRGFAVVASEVRNLAGRSAEAAKEIKALIQESVARVDEGTKLVDQSGATLGGIVNS
                     IKKVSDIVAEISAASQEQSSGIEQVNKAIMQMDGLTQQNAALVEEAAAAAESLQDQAR
                     GLLDSMSHFKVAGGSPAANETQRRAGTPRAVARSKAPREAQPKAAAVPMRKAAGAEGE
                     WSEF"
     gene            complement(8304..9233)
                     /gene="rsgA"
                     /locus_tag="VEW08_16235"
     CDS             complement(8304..9233)
                     /gene="rsgA"
                     /locus_tag="VEW08_16235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HYJ42342.1"
                     /translation="MTDPATADKFDAEVIAAFGRHLLVRSGTQELRARPAGRRLSIVC
                     GDRVRCEFDRAHDEVLAVEVLPRTSVLARSNLRGESEPIVANITQLVVVVAPLPQPDF
                     FIVDRYLCAATAAGIRGAVAINKSDLEANKIGREEIDAFAAAGYAHVTCSATDGQGLT
                     ELRALLAGAVSVLVGQSGVGKSSLVNALLPKEQVETGGLMREEEGRHTTTASRSYPLD
                     SGGTLMDSPGVRDFAPAIEQLDAETLGFPEIDRRARDCKFQDCRHMQEPACAVIAAVQ
                     AGEFLERRYESYRRLRRRYSDLIEARGPAHRRR"
     gene            9258..9815
                     /gene="orn"
                     /locus_tag="VEW08_16240"
     CDS             9258..9815
                     /gene="orn"
                     /locus_tag="VEW08_16240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HYJ42343.1"
                     /translation="MPSSDYLIWIDLEMTGLKPDTDVIIEMATIVTDIDLNVVAEGPV
                     IAIHQPPEVLDAMDEWNKRTHGASGLIDRVRSSSYTMASAEKRTLESLTLLVEPGSSP
                     MCGNSICQDRRFLARHMPTLEKFFHYRNLDVSTLKELARRWAPAIIPGFSKQGEHKAL
                     ADIQESIRELAYYRENFLAPEYQKL"
     gene            9812..10777
                     /locus_tag="VEW08_16245"
     CDS             9812..10777
                     /locus_tag="VEW08_16245"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYJ42344.1"
                     /translation="MSVKVPDFSDVVAAAARIGPHVVRTPVMRSAAFDARVGAQVFFK
                     CENLQTGGAFKYRGAMNVVLQLDPARTPLVVTHSSGNHGNALAMAARARGMRAIVVVP
                     RDSARSKIANIEAAGARVELCEPGLPAREKRLAEILATEPGELVHPFDDERIIAGQGT
                     AALELLAEVPSLDAIGTPVGGGGLIGGTALAAKGTVPHIRVIGAEPEQADDAFQSFRS
                     GERRAVVAPKTIADGLRGSIGVRNFELLRRLVDEVVTVSEVQIVEAMRIVLADFKLLI
                     EPSSAVAVAAVLAGKLGRPGERVGLVISGGNIDLDLCPFLRGERL"
BASE COUNT         2017 a         3456 c         3597 g         1937 t
ORIGIN      
        1 aggcggcgct ggacgccggg cttcgccgtc cgggcggggc tggcgcgtct ggcggagctc
       61 acagcgcttt cgccgcttcg aggacttcgg cggcgtgacc cttcacgcgg actttgcgcc
      121 actcgcgcgc gagcttgccg ttttcgtcga tgaggaatgt gctgcgctcg atgcccatga
      181 acttccttcc gtacagcgat ttctcgcgga tgacatcgaa gagcttgcag acggtttcgc
      241 cttcgtcgga aagcaactcg aacgggaatt tctctttcgc gcggaatttc tcgtgccgcg
      301 cgcaactgtc cttcgaaatg ccgagcaccg tcgtcttcgt gcggcggaac gcggcgtgca
      361 gatcgcggaa atcctggccc tcgagcgtgc agccgggggt catgtccttc gggtagaagt
      421 acagcacgag tttgccgccg cgcgcgtcag tggatttcca gagactgccg tcggagagcg
      481 gcagcgaaaa gacgggaacc ttctttccaa cgccgagagt caagacttca cgggctccag
      541 gatcgcgtcg aggttttgag cgtcgcagta gtccatgaac tcttcgcgca ggtgcgcgag
      601 ctgcaggcgc ttgggaacgt tgatgatcat ctgcacggcg aacatctgcg cgcccgtgtg
      661 cgccgcggga taggtgcggg tggagacttc ggcgatgtcg atgccgcggc tggcgcagaa
      721 cccggagagc ccggtgacga ttccggcctg gtccaggcac accacgtcga tggaatatgg
      781 caatagttcg gtgcggggtg cgcgcggctc ggtgcgcttc atgtgcagcg acaggccact
      841 ggcttcaccg aggcgagtca gctcgctttc gagcttggcg agcgaatgcc agttgccgct
      901 caccagcacg agcattgcga actcgttgcc gaggcttgcc atgcggctct cgctgatgga
      961 tccaccgcag tccgtgatta cgcgggtcag gtcatggacc ataccggtgc gatcgctgcc
     1021 aatggcggat acggcgaggt gttgcttcat ctggcggcga gtgtaccgca gccttttggc
     1081 ttgcgaatgg cgggtgtgcg cccgtaccat tcccgcccgt tcaaacgacc ctcggatttc
     1141 tcatgttcgc cggcagcctg gtggcgctcg tgaccccgat gctgccggat ggcagtatcg
     1201 attttgacgc ctggtcacgt ctcctcgaat tccacgtcgc caatggcacg gcgggaatcg
     1261 tcgttgccgg gtccacgggc gaatccgcga ccgtcaccga cggtgaattg cgcgacctgc
     1321 tggtccacgc gcgccgcgtg ctcggaaaac gagcctgggt catcgccaac gccggcacca
     1381 gcagcaccgc ttcgtcagtg gaacgcgcgc gctggatttc cgagctcggc atcgatgcgc
     1441 ttctgattgc gtctcccgcc tatgttcgcc cgacgcagca gggactcttc ctgcatttcg
     1501 aagcgattgc cgcgaactcg cgcattccgg tcctgctgta caacgtcccg agccggaccg
     1561 cgtgcgatat cctgccctcc acggttgcaa ggttgtctga agtcccgcgc atcgtcggta
     1621 tcaaggaagc catcggagat ggggcgcgtg tgcgggagct cgtcgccagt tgcgccgcgg
     1681 aattcaaggt actgagcggc gacgacctga ccgcgcgaga tgccatcggt ctcggagcct
     1741 ccggggtcat atccgtgacg gcgaatgccg cgccgcgtgc catggccgac atggtggccg
     1801 ccgcgctgcg gggcgaccgc gccggtgcaa cccagatcga ccagcggctc gtggccctgc
     1861 atcgcaacct gttcgtcgaa tccaacccca tcccggtgaa atgggcggta gcccgcatgg
     1921 ggctcatggg tggcaccctg cgcctgcctc tgaccgaact gtcggaggaa ttccatgcgg
     1981 tggtcaccga gtcgctgttt gctgccggta tcacagtcgc ccgatgaact cccgaagtcc
     2041 gacaaggtcg caatcctcat gcgtattccc acactcctga tcgcctgctg cctggccgta
     2101 ttggtcaccg gctgcaagtc tcacggcacc acctgcaaac aggacaacaa ggattacgtt
     2161 ggcgccgagg agatgccgcc gctgaaggcg cctcccggcc ttgagccgcc tgatacacga
     2221 aacgcgctca aggtcccgcc gcttgacacc cccgagcgtc cgcgcagcaa ggacgaggcc
     2281 tgcctcgatg tcccgccgcc atttaccaat cccaaggggc ccgacgcggc caagccgaag
     2341 tagagggact attggtatca ttcgcacccc ttgaaggccc ttcttgaatg ttttgggcgc
     2401 gggggtttgc gcgggtaacg gacagatggc cgagcggtcg aaggcgcttg cttggaaagc
     2461 aagtaacatc tcacggtgtt ccagggttcg aatccctgtc tgtccgccat ttctttacca
     2521 tggcagtccg tatcggctcc ttcgcgacga ctagttgtaa accccgtcag gcccggaagg
     2581 gagcagcggt agcttcgatg tcgggcgccg aagtccggct ggtgcgggct gcctcccaaa
     2641 gccaactcga gtaaggaact gcttcaacga tgagttatct cgcgctggcg cgtaagtggc
     2701 gaccgcgttc cttcgcggag ctcgttggtc aggagcacgt ccgacaggcg ctgggcaatg
     2761 ccctgacgca gggtcgtgtg caccacgcat tcctgttcac gggtacgcgc ggtgtgggca
     2821 agaccaccat cgcgcgcatc ctgtcgaagt gcctcaactg cgataccggc gtcaccgcca
     2881 ctccctgcgg cacctgcgca tcctgtaagg agatcgacga gggccgtttc gtcgacctca
     2941 tcgaagtcga cgccgcctcc cgcaccaagg tggacgacac gcgcgagctg ctcgagaacg
     3001 tgcagtactc acccacgcgc gggcgctaca aggtctacct cgtcgacgaa gtgcacatgt
     3061 tgtcgacgca ttccttcaac gcattgctga aaacgctcga agagccgcca ccgcacgtca
     3121 agttcctgct ggccacgacc gatccgcaaa agctgccggt caccgtgttg tcgcgctgcc
     3181 tgcagttcaa cctcaagcgc ctgcccgtct cgctgatcag cgagcacatg aacaaggtcc
     3241 tgactgccga gaaaattgct ttcgacgcgc cggcgctgcg gctggtggcg caagctgccg
     3301 acggcagcat gcgcgacgcg ttgtcgctgc tcgatcagct catcgcgttc ggcggcggca
     3361 aagtggaaga ggccagcgca cgcggcatgc tgggcaccat cgaccgcgac cacgtgacgc
     3421 gactcgcgcg tgcgctcgcc acgggcgatg ccgcgcagct tctcgaaacg gcgcgaagcc
     3481 tcgaggaatt ttcaccggac tacgggcagg tgctcgacga cctcgccggg ttgctcaccc
     3541 gggcggcact gcagcagctc gtgccaggat acgaaggcga tgaactgttc gatccgatgt
     3601 tgctgaaaga gctggcggcg gcaattcccg ccgaagacat gcagctttac taccagaccg
     3661 ccattctcgg ccggcgggac ctgccgttcg cgccggaccc acgcactggt ttcgaaatga
     3721 cgctggtgcg tatgctcgcg tttcgacctg caggcggcgc ggccgtggcg tcgactcccg
     3781 gtgggggcgg tgcgcgcgtg gctgcgagtg cgccgggcgg cgcgctgccc tcgaccacat
     3841 ccgcagccgt ggttcgaccg gcggccgcgg gtcccatcga ccccgtgcag tggtcgcgag
     3901 tcatcggcga tctcgacgtc agcggcgcgg cgcgccagct cgcgacgaac tgcgcgctcg
     3961 tggaacatcg cggcaaaacg ctgaagctcg cggtcgattc caaggtcgcg cgcacgccgc
     4021 cgcaggtcga gaagctggcg caggcgctgg caaaatacct gggcgggcac gtggtgctcg
     4081 aattcgtcga cggtgcgccg ccggtggaaa ccccggcgca aaccggcgag cggcgttcgg
     4141 cggaagcgct cgacatggcg cgtcgcgtgc tcgaagaaga tcccacggtg cgcgaaatga
     4201 aatcgcgttt cggcgccaca ctgcacccgg aatcggtacg gccaacggaa taggagtttg
     4261 aacgatgcgt ggcggcggaa acatgatgaa gcaggcgatg gcgctgcagg ccaacatgaa
     4321 gaaggcccag gatgaaatcg ccaacatgga agtcatcggt gaatccggtg gcggcatggt
     4381 caaggtgacc atgagcggaa aacacgaagt gaagcgggtg cagatcgagc cgcaggtcgc
     4441 ttccgaagat cgggaaatgc tcgaggacct gatcgccgcg gccgtcaacg atgcggtcaa
     4501 caaggtcgag cagcagagcc aggcgcgcat gtcgtcggtg atgtcgggcg tggcattgcc
     4561 acccggcttc aaaatgcctt tttaacgatg cagcacgccg ccccgctagc caaactgatc
     4621 gacgccctgc gcgcactgcc gggcgtgggg cccaagaccg cgcagcgcat ggcctttcac
     4681 ctgctgcagg gcggccgcgg cggcgcccgc gcgctcgccg acgcgctcag cgcggggctg
     4741 gcctcgatca agcgctgcca gcgctgccga atgctcaccg ataccgacct ctgttccatc
     4801 tgcgcggcgc cggcacgcga cgccgcgctg ttgtgcgtcg tcgagtcgcc cgccgacgtg
     4861 gtggccgtgg agcaatccgg cagctaccgc ggccgttatt tcgtgctgca cgggcacctc
     4921 tcgccgctcg atggcatcgg acccgagcag atcggggcgc gcgaattcga agcgttgctc
     4981 gactcgggtg aagtccagga agtgattctc gccaccaatc ccacggtcga aggcgagacc
     5041 accgctcact ttctcggaga gcgcgcggcc cagcgcgcaa tccgcgccag ccgcatcgct
     5101 cacggcgtgc cgatcggcgg cgagctcgaa tacgtcgacg gcggcactct ggcccatgcg
     5161 ctgaccggac ggatttccgt ctagttgtcc tttcaggcca cgcgaagcgc agcgtgaccg
     5221 aatccctcaa tagtttttcg tgacggtcga taaccgggag tgcgggttgt cggcttttcc
     5281 tgttctgctt aggggttcac ctatccagac cgggtcgcga gaaaaatcgc ggggtcgtgc
     5341 agcacgcatt tgtgcgtcat cgttcaatcc ggcagcaaag attcgcgtcc gttcctccga
     5401 gtgagacgca attctgccgc gttccgatcg atgagcttaa tgttccaaaa ggttgttagc
     5461 tggccgcgct ggtgcgcgcc gtgatcggga gaggacgggt aaatgagcaa attttcggaa
     5521 tccatgcagc gctggcttgg caacaagtcc atctcgcgcg tgatcatgat tctcatcggc
     5581 ctggcggtga cgccggcgct ggtcgtgacg gtatccatgg tgattcgcga gatcgcgcag
     5641 atcaaattcc tgcacgaaga atatcgggcg atcgtggagt ttcacccgct cgaggaaatc
     5701 acctcgcacg gcaccaaccg catgatcctc gggcagatgc ccgaggggca acgcgatgcc
     5761 gccaaatacg ctgaattcga tgcggaactc acgctggccg tcgaagaagt gaaggcagcc
     5821 gttgcgggcg ccggcaaacc ggcgttgcag ccgttctggg acgccgtcga agcgcagtac
     5881 gaaaaagtgc acgcgatggc gccgcaaagc gtctcgccgg acgaatggtt ccgcacgcac
     5941 gaggagctcg ccgagctgac cctgaagttg cgcgaccgcg tcggcgttga aaccggcgtg
     6001 gtcctcgatc ccggtgcgga gacgctgccg ctggtcgatg ccatgttcaa tcacatttcg
     6061 agcctggaaa cctccgtcat gcgcgcggct ggtcattcgt tcgtcgccgc gggcggcacg
     6121 atgtcggccg aggcatccga tggtctcgcg acggcggcca cccatatcga cgaattgact
     6181 gccgaaatcc acatggattt cgacgacgcc attctggcct cggccgatgg cgaggaaggt
     6241 tacaagagcg tggttgccga tcgtgatgcg acgctcaatg gcatgaaagt tctcgccgag
     6301 aaggtgcgcc agatgcgcgc ttccggaacg gccgcggacc acgcagcgat gatttcggcg
     6361 gcgttgacgg tgctcggcgc catcgacgaa ctgcacgatt ccggcagccc gaagctcgaa
     6421 gcgctgttgc gcgatcgcga gtcacgcacg cgctggatga ttgccctcgt ggccagtttc
     6481 ctgctgctgt tcatttcagt gtcggtctgg ctcggagtcc tcgcgagtcg tggtgtgtcg
     6541 cggtcgcttg gcatcgcggt cagcaccgcg gaaaacatcg cggcaggcag gtacgacaac
     6601 acgatcgaag cgcagggcga aaatgaaacc ggccgcgtgt tgcgcgccgt cggcgccatg
     6661 caggccgagt tgaagcggcg cacggaaaac gaaagcctgg cgcgtgagaa agagcgcgcc
     6721 gtcgccgcgg aaagcatgcg cgtcaaggtc ggcctcgaca gcgtatcggg caatgtgctg
     6781 ctggcggaca aggacaaccg catcatctat ctcaacaagg cgattcagca gatgtttcgc
     6841 gccgccgccg ccgacttccg gcgccagcgg ccgaacttcg acgcggaggc cctcatcggc
     6901 acgccgatgg atctgcacgg atccggcgct ggagccacgc gccacgcaca ccagacgcag
     6961 gtcaagattg gcagccgcac gttccgtgtc agcaccagcc ccgtagtcga cgatggcggt
     7021 ctgtggctcg gtaccgtggc cgaatggatc gatcgcacgc aggaagtggc ggtggaagag
     7081 gagatcgcga acatcgtcgt cgccgcttcg gcaggcgacc tttccatgcg cattcgcaag
     7141 gaaggcaagg atgggttctt cgccacgctg gccgatggca tgaacggcat cctgacaaac
     7201 atgtcggccg tggttgccga agtgaactcg gtcatcgaag ccggcaagaa ccaggacctg
     7261 acggcccgca tcgacatcgc cggtaaatcc ggcgtgttcg agcagctgtc gggcggcatc
     7321 aacgcgctca tggactccat gatgagcgtg gtgcagcaga tcaagtcggc ggcgcagtca
     7381 gtggcttcgg gctccgacga aatcgccaaa ggcaacgcgg atctcagcgg acgcaccgag
     7441 cagcaggctt cgtcgctgga agaaacggct tccagcatgg aagagatgac cagcacggtg
     7501 aaacacaacg cggacaacgc gtcgcaggcc aatcagctgg cgcgtgccgc gcgccaggaa
     7561 gccgagcagg gcggcgcggt ggtatcgaat gcggtcgccg cgatgcaggg catcaacgca
     7621 tcgagcaaaa agattgccga catcctgggc gtgatcgacg agatcgcgtt ccagaccaac
     7681 ctgctggcgc tgaacgccgc ggtggaagcc gcgcgagcgg gcgagcaggg ccgcggattc
     7741 gccgtggtcg cgagcgaggt ccgcaatctc gccggacgca gcgccgaggc agcgaaagaa
     7801 atcaaggcgc tgatccagga gagcgtcgcg cgtgtcgatg aaggcaccaa gctggtcgat
     7861 cagtcgggcg ccacgctcgg cggcatcgtc aactcgatca agaaggtgtc ggacatcgtt
     7921 gcggaaatca gcgcggcgag ccaggagcaa tccagcggca tcgaacaggt gaacaaggcc
     7981 atcatgcaga tggacggcct gacacagcag aacgcggcac tcgtcgaaga agccgccgct
     8041 gcagccgagt cgctgcagga tcaggcacgc ggactcctcg acagcatgtc tcacttcaag
     8101 gtcgcgggcg gctcgccggc ggcgaacgaa acccagcggc gtgcaggtac gccgcgcgcg
     8161 gtggccagat cgaaggcgcc gcgcgaggcc cagccgaagg cggctgcagt gcctatgcgc
     8221 aaggctgcgg gcgcggaagg cgagtggagc gagttctgat cggaactcgt gccactattt
     8281 aaccgtgaga tcggccggaa gtcttagcgg cggcgatgcg cgggcccgcg tgcctcgatc
     8341 aggtcggagt accggcggcg caaacggcga taactttcgt atcgccgctc cagaaactct
     8401 ccggcctgta cggcggcgat cactgcgcag gcgggttcct gcatgtggcg gcagtcctgg
     8461 aacttgcagt cgcgcgcgcg ccggtcgatt tccggaaagc cgagagtttc cgcgtcgagc
     8521 tgctcgatgg cgggcgcgaa atcgcgcaca cccggagaat ccatcagcgt gccgccggaa
     8581 tcgagtggat acgaacgcga ggcggtcgtg gtgtggcggc cttcctcttc gcgcatgagt
     8641 ccgccggtct cgacctgttc ctttggaagc agcgcgttca ccagcgacga cttgccgacg
     8701 ccagactgac cgacgagcac actgaccgcg cccgcgagca gtgcgcgcaa ctcggtgagg
     8761 ccttgcccgt cggtcgcgga gcaggtgacg tgggcgtagc ccgcggccgc gaatgcatcg
     8821 atctcctccc ggccaatctt gttagcctcg agatcggact tgttgattgc cactgcgccg
     8881 cggatgccgg cggccgtcgc cgcgcacagg tagcggtcga cgatgaaaaa atccggttgc
     8941 ggaagcggcg ccacgacgac cacgagttgc gtgatgttgg cgacaatcgg ttcgctttct
     9001 ccgcgcaggt tggagcgcgc cagcacgctc gtgcgcggca gcacttcgac cgccagcacc
     9061 tcgtcgtgcg cgcggtcgaa ttcgcagcgg acgcgatccc cacaaacgat gctcaagcgg
     9121 cgccccgccg gccgggcgcg cagctcctgc gtcccgctgc gcaccagcag gtgacggccg
     9181 aacgccgcga tgacctcggc atcgaatttg tcggcggtgg cgggatcggt cattcgtgta
     9241 gtatcgcgtc attcattatg ccgagcagcg actatctcat ctggatagat ctcgagatga
     9301 cgggcctcaa gcccgacacc gacgtcatca tcgagatggc taccatcgtc accgacatcg
     9361 atctcaatgt cgtcgccgaa ggaccggtca tcgccatcca ccagcctccc gaagtgctgg
     9421 atgccatgga tgaatggaac aagcgtacac atggcgcctc gggcctcatc gaccgcgtgc
     9481 gctccagcag ctacaccatg gccagcgcgg aaaaacgcac gctcgaatcc ctgacgttgc
     9541 tggtggagcc gggctcgtcg cccatgtgtg gcaacagcat ctgtcaggat cgccgcttcc
     9601 tcgcgcgcca catgccgacg ctcgagaaat tctttcacta ccgtaacctg gacgtgagca
     9661 cgctcaagga attggcgcgc cgctgggcgc ccgcgatcat cccgggcttc tccaagcagg
     9721 gtgagcacaa ggcgctcgcc gacatccagg aatccattcg cgagcttgcg tattaccgcg
     9781 agaatttcct cgcgccggaa taccagaagt tgtgagcgtg aaggttccgg atttctccga
     9841 tgtcgtggcg gcggccgcgc gcattgggcc acacgtggtg cgcacacccg tcatgcgttc
     9901 cgcggcgttc gacgcacgcg tcggcgccca ggttttcttc aagtgcgaaa acctgcagac
     9961 cggcggtgcg ttcaagtatc gcggcgccat gaatgtggtg ctgcaactcg atcctgcgcg
    10021 cacaccgctc gtggtcactc attcttccgg caatcacggc aacgcactcg cgatggcggc
    10081 gcgcgctcgt ggcatgcgcg cgattgttgt agtgcctcga gattccgcga gatcaaaaat
    10141 tgccaacatc gaggcggccg gtgcgcgtgt ggaactgtgt gagcccggat tgccggcgcg
    10201 cgagaaacgg ctggccgaga tccttgcgac agaacccggc gagctcgtgc atccgttcga
    10261 cgacgagcga atcattgcgg ggcagggcac cgcagcactc gagttgctgg cggaagttcc
    10321 atcgctcgat gccatcggca cgcccgtagg tggcggagga ctgatcggcg gcactgctct
    10381 cgccgccaag ggtacggtcc cgcacattcg cgtcattggc gcggagccgg aacaggctga
    10441 cgatgccttt cagtcatttc gaagcggcga acgtcgcgcg gtagtggcgc ccaaaaccat
    10501 tgccgacggg ctgcgcgggt cgatcggcgt gcgcaacttc gagctcctgc ggcggctcgt
    10561 ggacgaggtg gtcacggtca gcgaggttca gatcgtcgag gcgatgcgca tcgtgctcgc
    10621 cgatttcaaa ttgctcatcg aaccctcatc ggcggttgcc gtggcggcgg tacttgccgg
    10681 gaaactcggg cggcccggcg agcgagtcgg gctggtgatt tccggtggga acatcgacct
    10741 cgacctgtgc ccgttcctgc ggggcgagcg cctataagaa gagtcgggaa tgtaaagaat
    10801 agttgacatc tgcgtatcga gatgtaaggt ataccggaca ttcaccacgc cgggataccc
    10861 gccatgacca ctcttcaccg ccgcagcgtc ttgctgctct gcctcgccgc attaggcgcg
    10921 gctggcatgt ttctgggtcc cgatggctgg ttcggcatcg atatcgggcc cgtcggctct
    10981 gtggtcctgt acgccgcgtt gtggctg
//
LOCUS       DASQJS010000411         5336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2779768, whole genome shotgun sequence.
ACCESSION   DASQJS010000411 DASQJS010000000
VERSION     DASQJS010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2779768"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VEW08_16250"
     CDS             complement(<1..976)
                     /locus_tag="VEW08_16250"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HYJ42345.1"
                     /translation="MRPLIVLIVLCCAGVAQADVFRPAYLELRETGTDRYDVTWKVPV
                     RGDTRLGVKVVFPPGTEQASAPQALFTPDAYTERWKVVRVGGFAGQTLTIDGIASGVT
                     DVIVRIEHADGTSQVEQLLPQRPRFTVAAVTSTAAIAWSYLVLGVEHILGGVDHLLFV
                     LALLLIVSGGKRILVTITAFTLAHSITLVAATLGWVHVPGPPVEAMIALSIVFVAAEI
                     VRGLRGSPGLTARAPWVVAFSFGLLHGLGFAGALAEVGLPQKAIPMALFTFNVGVEIG
                     QLMFVTVALVAGALLRRTAISRPTWAPYAAPYAIGAIAMFWVIERIGAF"
     gene            complement(988..1830)
                     /locus_tag="VEW08_16255"
     CDS             complement(988..1830)
                     /locus_tag="VEW08_16255"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYJ42346.1"
                     /translation="MIRRLLREPLLHFFLLGTVLFGVYGWLNRGGASAPTEIFVSRDQ
                     VRSFEAQFERVWRRPATPQELQHLVDNWVREEIFYREGLALGLDRDDPVVRRRVGQKV
                     EFIIDGATPAAPTTAELQAWLDAHPASYTIEPVYSLQQVYFDPQRHGASLDRDIAAAQ
                     RSLASGKVVIGDATMLPAALEATAQFDVEREFGDAFAAALKDLPVGSWQGPVKSGFGL
                     HLVLLSERSGGTRPQLEQVRAEVERDLLRVRAKEANEAMYDRLRANYRVLVESAGTPA
                     DPAG"
     gene            complement(1839..3719)
                     /locus_tag="VEW08_16260"
     CDS             complement(1839..3719)
                     /locus_tag="VEW08_16260"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HYJ42347.1"
                     /translation="MAHAQQASEIITDIADVQPADPKKFYKEPGYSPYAGKHYPSRPL
                     FGDQHLHTGWSVDAGLGGATLTPEDAVRFARGEQVTSSSGQPVRLSRPLDWVAVTDHS
                     DAMGTIAAIREGNPEMMKDPTLKRWHDMMAQGPEEGTKATMEVVAAQANKKMPPALVD
                     PKWAKTYWDKNIDIMEKYNDPGRFTALIAYEWTSNAGGGNNLHRNVIYRDNKDRARQV
                     LPMTTFQSENAEDLWKWMADWEKKTGGQLLAIPHNGNLSNGRMFELQMFMGGPMTKDW
                     AQQRQKYEPLFEAIQMKGQSESHPSLSPTDEFAVGYEIWDRGNLALVPKKPGMIEQEY
                     LRQALKAGLKVEQDLGANPFKYGMAGGTDTHNGLTAAEEDNFYGKFVGAEPRRDRWEE
                     DAIKFGDRVVKGWEMTAAGYTGVWATENTRAAIWDAMKRRETYATSGPRMIVRFFGGY
                     EFTKRDAALRSPAIAGYNKGVPMGSDLPKAPADKAPTFLIAAVKDPFSGNLDRIQVIK
                     GWLDKSGKLQEKIFNVAWGDAETRKLQANGKLPAVGNTVDLATASWTNTIGDPELITV
                     WKDPEFNPALRAFYYARVLEIPTPRWTAYDAAYFQVKITDPKVPMTTQERAFTSPIWY
                     SP"
     gene            3909..4826
                     /locus_tag="VEW08_16265"
     CDS             3909..4826
                     /locus_tag="VEW08_16265"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HYJ42348.1"
                     /translation="MKSAARLAGVVCCACCARAALGQELEPRAYSPAPTGVNFMLFAG
                     VRSTGEVLTDPSLPVENVEATIDAGALAYGRTFAVAGHSASLALAVPYVWADLTGDVG
                     EERQSVSRVGVADTKLRLAVNLLGGAAMTPKEFAKRTPQTTLGASLSIVSPTGEYFPD
                     KLINIGTNRWAFKPEVGLSIPHNRWMFDAYAGVWLFTDNSDFYGGVRREQDPMPTLQA
                     HVSYTFRPGLWIAFDSTYYFDGETTVNGVPKNDRKDNSRAGLTLSLPMARWHALKFSW
                     SEGASTRTGNDFSTFGIAYQYTWFDKPKP"
     gene            complement(4827..>5336)
                     /locus_tag="VEW08_16270"
     CDS             complement(4827..>5336)
                     /locus_tag="VEW08_16270"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HYJ42349.1"
                     /translation="SVQLDPVKIEFSKNWEKNSVSGSSLPFSRRPTAEDMQRIREGLA
                     ELMQEVFTEELTKHGYTLVDTPAEDTLHVLTAIIDLYINAPDNSDPGITRTYTASSGS
                     MTLVLEARDGPTGQLLARVVDGRSDDSPGGTFQWTNRATNTQAARQILQVWARKLRES
                     LDRLNGKEK"
BASE COUNT          913 a         1706 c         1681 g         1036 t
ORIGIN      
        1 tgaaggcgcc gatccgttcg ataacccaga acatcgcgat ggcgccgatg gcgtagggcg
       61 ccgcatacgg tgcccatgtc ggcctggaaa ttgccgtgcg ccgcaacagg gctccggcca
      121 cgagtgcgac ggtgacgaac atcagttgac cgatctcgac acccacattg aaggtgaaga
      181 gagccatcgg aatcgccttc tgtggcagcc ccacttcggc gagtgcgccc gcgaagccca
      241 gcccgtgcag cagtccgaag ctgaatgcga caacccacgg cgcgcgggca gtgaggccgg
      301 ggcttccgcg caagccacgc acgatttcgg cggccacgaa cacgatgctg agcgcaatca
      361 ttgcctcgac gggcgggcct ggcacgtgaa cccagcccag ggtggcggcg accagcgtga
      421 tgctgtgcgc cagcgtgaag gcggtaatcg tcacgagaat gcgtttgccg ccgctcacga
      481 tcaaaagcaa cgccagcacg aacaacagat gatcgacgcc gccgaggatg tgttcgacgc
      541 cgagcacgag ataggaccat gcgattgctg ccgtgctggt gacggcggcg acggtgaatc
      601 gcggccgctg tggcaggagc tgctccacct ggctggtgcc atcggcgtgt tcgatgcgca
      661 cgatgacgtc cgtgacgccg ctggcgatgc cgtcgatggt caacgtctgt ccggcgaaac
      721 cgccgacgcg gaccaccttc cagcgttcgg tataggcgtc gggggtgaac agggcctggg
      781 gcgcagatgc ctgctcggta cccggaggga acacgacctt cacgccgagg cgcgtatcgc
      841 cgcgcaccgg caccttccac gttacgtcat agcggtccgt gccggtttcg cgcaactcta
      901 ggtaagcggg gcgaaaaaca tccgcctgcg caactcccgc gcaacagagc acgatcaata
      961 caatcagggg acgcaccagc atgtgattca gcccgccggg tccgccggcg ttccggccga
     1021 ttcgacgagc acacggtagt tggcgcgcag gcggtcgtac attgcctcgt tggcctcctt
     1081 cgcgcgtacg cgtagcagat cccgctccac ttccgcgcgc acctgctcga gttgtgggcg
     1141 ggtgccgccc gagcgctcgc tgagcaacac gaggtgaagg ccgaagccgg atttcaccgg
     1201 tccttgccag ctgcccacgg gaagatcctt cagcgcggct gcgaacgcgt cgccgaattc
     1261 ccgctcgacg tcgaattgcg ctgtggcctc gagcgcagcc gggagcatcg tcgcgtcgcc
     1321 gattaccacc ttgccactcg caaggctgcg ctgcgccgcg gcaatgtcgc gatccagcga
     1381 ggcgccgtgc cgctgcgggt cgaagtacac ctgctgcagc gaatagacag gctcgatggt
     1441 gtagctcgca ggatgcgcat cgagccaggc ctgcagctcg gccgtggtcg gcgctgccgg
     1501 ggttgctcca tcgatgatga actcgacttt ctgacccacc ctgcgcctta ctaccggatc
     1561 gtcacgatcg agtccgagcg ccagcccctc gcggtagaag atttcctcgc gcacccagtt
     1621 gtccacgagg tgttgcagtt cctgcggcgt cgccgggcgc cgccagacgc gctcgaactg
     1681 cgcttcgaag ctccgcacct gatcgcgcga gacgaatatc tccgtgggtg ctgatgcacc
     1741 gccgcggttc agccagccat acacgccgaa caagaccgtt cccagcaaaa aaaaatgcag
     1801 gagcggctcg cgcagcaaac gtcgaatcac ggcccctttc acggggaata ccagatcggc
     1861 gaagtgaacg cgcgctcctg cgtggtcatc ggcaccttgg gatccgtgat cttcacctgg
     1921 aaataggccg cgtcataggc ggtccagcgt ggcgtcggaa tttccagcac gcgcgcgtag
     1981 tagaacgccc tgagggccgg attgaattca ggatccttcc agacggtgat cagctctgga
     2041 tcaccaatcg tgttcgtcca gctggccgtc gcgagatcca ccgtattgcc cacggcgggt
     2101 agtttgccgt tggcctgcag cttgcgcgtt tccgcgtcgc cccaggcgac gttgaagatc
     2161 ttctcctgca gtttaccgct cttgtccagc catcccttga tcacctggat gcgatcgagg
     2221 ttaccgctga atggatcctt cacggcggcg atcaggaagg tcggggcctt gtccgcgggc
     2281 gccttcggga ggtcgctgcc catcggcacg cccttgttgt agccggcgat tgccgggctt
     2341 cgcaatgccg cgtcgcgctt cgtaaattcg tagccgccga agaagcgcac gatcatgcgc
     2401 ggcccgctgg tcgcgtacgt ttcgcggcgc ttcatcgcgt cccagatggc ggcgcgcgtg
     2461 ttttccgtcg cccacacccc ggtgtaaccg gcggcggtca tctcccagcc cttgacgacg
     2521 cgatcgccga acttgatcgc atcctcttcc cagcgatccc tgcgcggctc ggcgccgacg
     2581 aacttgccgt agaaattgtc ttcttccgcg gccgtgagtc cgttgtgtgt gtccgtgcct
     2641 ccggccatgc cgtacttgaa gggattcgcg ccgagatcct gctcgacctt gagtccagcc
     2701 ttgagggcct gccgcaggta ctcttgttcg atcatgccgg gcttcttggg cacgagcgcg
     2761 agattgccgc ggtcccagat ttcatagccc accgcgaatt cgtcggtcgg cgacagcgag
     2821 ggatgtgatt cgctctggcc tttcatctgg atggcctcga acagcggctc atatttctgg
     2881 cgctgctggg cccaatcctt cgtcatgggg ccacccatga acatctgcag ctcgaacatg
     2941 cggccattgg agagattgcc gttgtgagga atggccaaca gctggccacc ggtcttcttc
     3001 tcccaatccg ccatccattt ccacaggtct tcggcgtttt cgctctggaa cgtggtcatg
     3061 ggcaggacct gccgcgcgcg atccttgttg tcgcgataga tcacgttgcg gtggagattg
     3121 tttccgccgc cggcgttcga ggtccactcg taggcgatga gcgccgtgaa acggccggga
     3181 tcgttgtatt tctccatgat gtcgatgttc ttgtcccagt aggtcttcgc ccacttcggg
     3241 tcgaccagcg ctggtggcat cttcttgttg gcttgtgcgg ctaccacctc catggttgcc
     3301 ttggtgcctt cctccggtcc ctgcgccatc atgtcgtgcc agcgcttgag cgtcgggtcc
     3361 ttcatcatct ccgggttgcc ttcgcggatc gcggcaattg tgcccatggc gtcggaatga
     3421 tctgtgaccg cgacccagtc gagcggtcgc gaaagcctga cgggctgacc ggatgacgag
     3481 gtgacttgct ctccacgagc gaagcgcacg gcgtcctccg gcgttagcgt cgcaccgccc
     3541 aggccagcgt cgacggacca gccggtatgc aggtgctggt cgccgaacag cggacgtgac
     3601 gggtagtgct tgccggcata cggcgagtat cccggttcct tgtagaactt cttggggtcc
     3661 gctggctgaa catcagcgat gtcggtgatg atctcgctgg cctgctgggc gtgcgccaca
     3721 ggggccaagc atagaaacga cgccaggctt acgccgacgg atgcggccat tgctttcatt
     3781 tgttggtccc tgtttgagtg atcaatgtgc gggcgcacct gttagacaat atcgaagggc
     3841 aggcgaccgc ccgaaatggg accttggtcc aaggagcgga atgcagcgcg acatggctga
     3901 cttggcgcat gaaaagcgcc gcgcgtcttg cgggagttgt gtgctgcgcg tgctgcgcca
     3961 gggccgcatt gggccaggag ctcgaaccgc gcgcgtactc gccggccccg accggcgtga
     4021 acttcatgct gttcgcgggt gtgcgctcga cgggcgaagt cctgaccgac ccatcgttac
     4081 cggtggaaaa cgtcgaagcc actatcgacg ctggcgccct ggcgtacggg cgcacgttcg
     4141 cggtggcggg ccattcggcg agcctggcgc tcgcggtccc ttacgtgtgg gccgacctca
     4201 ccggtgacgt gggagaagaa cgtcagtccg tgtcgcgagt gggcgtcgcc gataccaagc
     4261 tgcggctcgc cgtgaacctg ctcgggggcg cggccatgac cccgaaggaa ttcgcgaaac
     4321 gcacgccgca gaccacgctc ggcgccagtc tcagcatcgt ctcgcccacc ggcgaatact
     4381 ttcctgacaa gctcatcaac atcggcacga atcgctgggc gttcaagccc gaggtgggcc
     4441 tgtcgattcc gcacaaccgc tggatgttcg acgcgtacgc gggagtctgg ctgttcaccg
     4501 acaattccga tttctacggt ggagtccgcc gcgagcagga tccgatgccc accttgcagg
     4561 cgcacgtgag ctacacgttt cgcccgggac tgtggatcgc cttcgacagc acctattact
     4621 tcgacggcga gaccacagtc aacggcgtgc cgaagaatga tcgcaaggac aactcgcgcg
     4681 cgggactgac attgtcgctg cccatggcca gatggcacgc gctgaagttc agctggagcg
     4741 agggcgcgag cacgcgcacg ggcaacgact tctcgacctt cggcattgcg tatcaataca
     4801 cctggttcga caagcccaag ccctgactac ttctccttgc cgttgagccg gtcgagactc
     4861 tcgcgcagct tcctggccca cacctgcaag atctgccgtg cggcctgggt gttcgtcgcc
     4921 cggttggtcc actggaacgt accgccggga ctgtcgtcgg aacgtccgtc gacgacacgg
     4981 gccagcaatt gccctgtcgg cccgtcgcgc gcttcgagca ccagcgtcat ggaccccgag
     5041 ctcgccgtat aggtgcgcgt gatgccggga tcggagttgt ccggcgcatt gatgtagaga
     5101 tcgatgatgg ccgtgagcac gtgcagcgtg tcttccgccg gcgtatccac gagcgtgtag
     5161 ccgtgcttcg tgagttcctc ggtgaagact tcctgcatca gttcggccag gccttccctg
     5221 atccgctgca tgtcttcggc agtcgggcgg cgactgaagg gaagactgct gccgctgact
     5281 gaattctttt cccaattctt ggaaaactcg atcttcaccg ggtcgagctg cacact
//
LOCUS       DASQJS010000412         7042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1835394, whole genome shotgun sequence.
ACCESSION   DASQJS010000412 DASQJS010000000
VERSION     DASQJS010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835394"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VEW08_16275"
     CDS             complement(<1..427)
                     /locus_tag="VEW08_16275"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HYJ42350.1"
                     /translation="MLGGGAAGFTLSRLAGAQDGSVVAAEKTPLEERSLELFNTHTNE
                     RAHVVFKRGAEYDPVALETFKKLLRDHRNGEAHDMDPRLYDQLFELAVAAKCHPHYEI
                     ISGYRSAESNDKMSSKPGSGVAKKSLHMQGRAMDVRLRNC"
     gene            complement(662..1096)
                     /locus_tag="VEW08_16280"
     CDS             complement(662..1096)
                     /locus_tag="VEW08_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42351.1"
                     /translation="MRTFIRMLVLLPALILGSCGGGGGSDEGQGPLPASVLGQEYLIG
                     FGETIHVGSLALEFTTLAEESRCPLNANCVWAGNARILVTATLGGTTEVMELNTYAGY
                     QVRSTFQGYLIELRRLQPDIPWAPRGPLEDYTATLFVDRASN"
     gene            complement(1203..2402)
                     /locus_tag="VEW08_16285"
     CDS             complement(1203..2402)
                     /locus_tag="VEW08_16285"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HYJ42352.1"
                     /translation="MLTKTRNSRATRSFIDPFTAPEIKRLVAEFGSPLLIIDCQRVRE
                     QYRKLHKALPGVDLHYALKPLPHPAVVQTIVELGGWLDLATTGETQLVTQLGVDPARC
                     IHTHPIKRDIDIRNALHLGVKIFVADNPDEVRKFEPYRDECELLLRVSFRSPGAVCDL
                     SRKFGCDPEDLLELARLARKLAIPVRGLSFHVGSQASDAGKHVEAIEACGKLIAAARR
                     EKLGVLDTLDIGGGFPIEYAQKVADIGRFCAPIRAALAKLPKKLRIIAEPGRYISGPA
                     AIGVSSVMGRAKREGHWWYYLDDGLYGSYSGQLFDHAKYPVESLKQSDEKFPSVLAGP
                     TCDSIDVIAENMMLPALKAGDLIVGRAMGAYTWASASEFNFFPKATVVSVNSKPGDTG
                     SVVPLPF"
     gene            2523..4031
                     /locus_tag="VEW08_16290"
     CDS             2523..4031
                     /locus_tag="VEW08_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYJ42353.1"
                     /translation="MKSTAPDITPAGELQTLMASRVALVVIESREEARVIELVREAAL
                     KAQRGKNWGVFQWTVTEGLLRVDIDLGGAQKTLAQPDQLLKHIRATTMAGIYVLLDFH
                     PYLDSNPTFVRMLKDIAQDYKKCARTLVLISHEVKLPQELDHLAARFSLRMPDKTERQ
                     AIVMKMAREWAQGAGGMPRVDMKVVEKLVDNLAGLPLHDVERLTRQAIFNDGALTEDD
                     LRPLMAAKYQLLNRGGTLSFEVDTARFAEVGGMKNLRRWILQRKAAFDGSAPELDAPK
                     GVLLLGVQGCGKSLAARAAAGVLGVPLVRLDFGALYSKWHGESEKNLRESLSSAEALS
                     PCVLWLDEIEKALSTGDGDSGTSRRVLGAFLTWLAEQRARVFIVATANDITALPPELV
                     RKGRFDEIFFVDLPTAAARLEIFAIHCKKRAVALTEPEMKALAARSEGFSGAEIEQAV
                     VSALYTAHANKQVVEAKLIAAELDATKPLSVVMGEKIAELRAWAAERTVPAD"
     gene            complement(4078..5643)
                     /locus_tag="VEW08_16295"
     CDS             complement(4078..5643)
                     /locus_tag="VEW08_16295"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HYJ42354.1"
                     /translation="MDLTMAFLPIVVLVVLVFFLIAFLATRYKRCPSDKILVVFGKVG
                     KGQSAMCVHGGGKFIVPLFQDYTYMSLTPLTIPIPLTKALSLQNIRVNVPSTFTVGVS
                     TEEAIMNNAAERLLNLAQDDVESMAREIIFGQLRLTVASLTIEQINQDREKFLDAIRR
                     NVAPELNKIGLYLINVNITDITDESGYIESIGKKAASEAINQAKVDVSDQDRKGSVGE
                     AEQVRERTIKVAQHLAESEKGKKQAEADKRIYVQQQEALATIGEAEANRDLQIKVAQN
                     EATSIQGQKKAEAEKRIYIEAQEAEAISGENRAKALIAASNAELFVQSALASQRGQVA
                     QFDAEAEIQRAQAKAELQRLTAQEVVRQQVEKTKITIAAEAEAEKTRREAQGVADATL
                     LKYEAEAKGIRQVLEAKAQGYQALVASANNDARAAATLLLTEKLEHLVAMQVEAIKAI
                     KIDKVTVWDAGAGANGNGGTTTSNFLAGMVKSLPPLHDVAAMAGLDLPKFLGDANVLS
                     KLDGNGSLIPPPK"
     gene            complement(5693..6250)
                     /locus_tag="VEW08_16300"
     CDS             complement(5693..6250)
                     /locus_tag="VEW08_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HYJ42355.1"
                     /translation="MTEWFASYNSVEQLFLLAGIVGGIVVVFRLILTVAGLDHHGGLD
                     AAHMDTDAGFQALTIQGISSFFTMFGVVGFVLYHNAALGLFLALLGAVGAGVFSVWLI
                     QKIFRSMMRLQSSGTVSLFAAVGSEGSVYLTVGKDGGRVQINFANRLREFEAVSADGS
                     TLPTGTAIRVQSVTANTLVVSPVER"
     gene            complement(6314..>7042)
                     /locus_tag="VEW08_16305"
     CDS             complement(6314..>7042)
                     /locus_tag="VEW08_16305"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HYJ42356.1"
                     /translation="LEAALADMPRQIGGKPTAVLMISAHWEESEFTLTSNPKPPMIYD
                     YGGFPDYTYHIHYDAPGDPVLAARVKSMLDAAGLPARLDAERGFDHGAFTPLKVIYPD
                     ADVPVVQLSLKRGLDPATHLAMGRALAPLRDEGVLIVGSGLSYHNLRQFFSPAAYGPS
                     RQFDAWLIGALLGGSPADRGKLLTAWETAPSARAAHPREEHLLPLMVAVGAAGDDMTE
                     LAYHEKEFLGGITVSSFRFMDSDY"
BASE COUNT         1294 a         2251 c         2191 g         1306 t
ORIGIN      
        1 cgcagttcct gagtcgcaca tccatcgctc ggccctgcat gtgcaacgat ttcttcgcga
       61 caccgctgcc cggcttcgag gacatcttgt cgttgctctc ggctgagcga tagccggaga
      121 tgatttcata atgcgggtgg cacttcgcgg caacggccaa ctcgaacagc tggtcgtaca
      181 ggcgcggatc catgtcgtgc gcttcgccgt tgcgatgatc acgcagcagt ttcttaaagg
      241 tctcgagcgc gaccgggtcg tactccgcgc cgcgcttgaa caccacgtgc gcgcgttcat
      301 tggtgtgggt attgaatagc tcgagactcc gctcttcgag cggggtcttc tccgccgcga
      361 cgacggatcc atcctgcgcg cccgccagac gcgacagcgt gaacccggcg gcaccgccac
      421 caagcacttt caaccaatgc cttcgtgtcc agatcacatt catttcccag cctcaaccag
      481 cggcgtagcc acgaaatcct tgcttggaac gccagccatc ggcattggtt ccaaatcgca
      541 caactcaatg gcaataccct tgctgttttc cctgtagcca cgcatgacca cagtctgcgg
      601 gcggcggagc gcccctacaa gaatgagaac gggagcgaag tcacagtgcc cgtattcggg
      661 ctcagttgct ggcgcgatcc acgaacaagg ttgccgtgta gtcctccaac ggaccccgcg
      721 gcgcccacgg aatgtcgggc tgcaacctgc gcaactcgat gaggtagccc tggaaggtgg
      781 accgcacctg atagccggca taggtgttca gctccatcac ttccgtcgtg ccgcccaacg
      841 tggcggtcac gagaatccgg gcattgccag cccacacgca attcgcattg agggggcagc
      901 gcgactcttc cgcgagcgtc gtgaattcca gggccaatga accgacgtga atggtctcgc
      961 cgaagccgat cagatattcc tggcccagca ccgaggccgg aagcggcccc tgcccttcgt
     1021 cgctgccacc gccaccgccg caggatccca ggatcaacgc cggcagcagg accaacatac
     1081 ggatgaatgt gcgcatgggc ggcatcctgc acccgcccgc ggcgcaccga caagtgacta
     1141 actaaccgga tcttgtaccg aaattgtcag cggtggatgc caccgacaca gaaaagcaca
     1201 gatcagaacg gcagcggcac caccgaaccc gtgtcaccgg gtttgctgtt caccgagacc
     1261 acggtggcct tcgggaagaa gttgaattcc gacgcgctgg cccaggtgta ggcccccatc
     1321 gcacgcccca ctatcaggtc tccggctttc aacgccggca gcatcatgtt ttcagcaatc
     1381 acatcgatgc tgtcgcatgt gggacccgcc agtaccgacg ggaatttctc gtcgctctgt
     1441 ttcaacgact ccaccgggta cttcgcgtga tcgaacaact ggccgctgta cgaaccgtac
     1501 aaaccgtcgt ccaggtagta ccaccagtgg ccctcgcgct tggcgcgccc catcactgaa
     1561 gacacgccga tggccgcggg cccggaaatg tagcggccgg gttcggcaat gattcgcagc
     1621 ttctttggaa gcttcgcgag cgcggcgcga atcggcgcgc agaagcggcc gatgtccgcc
     1681 accttctgtg cgtactcgat gggaaagccg ccaccgatat cgagcgtgtc gagcacaccc
     1741 agcttttcac gcctggccgc ggcgatgagt ttgccgcagg cctcgatggc ctcgacgtgt
     1801 ttgccggcat cacttgcctg cgagccgacg tggaacgaca acccgcgcac cgggatcgcg
     1861 agcttgcgcg ccagccgtgc gagctcgagc aggtcttccg ggtcgcaacc gaacttcctc
     1921 gacaggtcgc acacggcgcc gggcgagcgg aacgacacac gcagcagcag ctcgcactca
     1981 tcccgatagg gttcgaactt gcgcacctcg tcggggttgt cggcgacgaa gattttcacg
     2041 ccgagatgca gcgcgttgcg gatgtcgata tcacgcttga tgggatgcgt gtggatgcag
     2101 cgggccgggt cgacgccgag ctgggtcacc agctgcgtct cgcccgtggt agccaggtct
     2161 aaccagccac cgagctcgac gatggtctgc accacggcgg ggtgtggcag aggcttcagc
     2221 gcatagtgca ggtcgacacc gggcaatgcc ttgtgcagct tgcggtactg ctcgcgcacc
     2281 cgctggcagt cgatgatgag cagcggcgaa ccgaattcgg ccaccagacg cttgatctct
     2341 ggcgcggtga acgggtcgat gaaggaacgt gtcgcgcggg aattgcgcgt cttggttaac
     2401 accgctgtct cctgccggaa cggccggcgg aaaagcggcg cattgtaggc gggcgtggtg
     2461 tcggtgtcat ggggcgtgaa cccaagcccc tgaatagggc gttttgctaa agtgccgcgc
     2521 acatgaaaag cacggctcct gacatcaccc ctgccggaga actccagacc ctgatggctt
     2581 cccgcgtggc gctggtggtc atcgaaagcc gggaagaagc ccgcgtcatc gagctggtgc
     2641 gcgaagccgc cctgaaggcc cagcgtggca agaactgggg tgtctttcag tggaccgtga
     2701 ccgagggact cctgcgcgtc gacatcgacc tgggcggagc gcaaaagaca ctggcgcaac
     2761 cggatcagct gctgaagcac atccgcgcca ccacgatggc cggcatctat gtgctgctcg
     2821 acttccatcc ttatctcgac agcaacccga cgttcgtgcg catgctcaag gacatcgcgc
     2881 aggactacaa gaaatgtgcg cgcacgctgg tgctgatcag ccacgaagtg aaactgccac
     2941 aggagctcga tcatcttgcg gcgcgcttct cgctgcgcat gccggacaag acggaacgtc
     3001 aggccatcgt catgaaaatg gcgcgcgaat gggcgcaggg cgcgggtggc atgccgcggg
     3061 tcgacatgaa agtggtggag aaactcgtcg acaacctggc ggggctgccg ctgcatgatg
     3121 tcgagcggct gacgcgccag gccatattca acgatggggc gctcaccgaa gacgacctgc
     3181 ggccgctgat ggcggcgaag taccagctgc tgaatcgcgg cggcacgctg tcctttgaag
     3241 tcgacacggc gcgcttcgcc gaggtcggcg gcatgaagaa tctccggcgc tggatcctgc
     3301 agcgcaaggc cgcgttcgac ggcagcgcgc ccgagctcga tgcacccaag ggcgtgttgt
     3361 tgttaggtgt acagggctgc ggcaagagcc tggcggcgcg cgccgcggcc ggcgtgctcg
     3421 gcgtgccgct ggtgcggctg gatttcggcg cgttgtattc gaaatggcac ggcgaatccg
     3481 agaagaatct gcgggagtcg ctgtcatccg ccgaagcgct ctccccctgc gtgttgtggc
     3541 tcgacgagat cgagaaggcg ctgtccacgg gtgacggaga cagcgggacg tcgcgccggg
     3601 tccttggcgc cttcctcacc tggctcgccg agcagcgcgc gcgcgtgttc atcgtcgcca
     3661 cggccaacga catcaccgca ttgccgcccg aactcgtgcg caagggccgc ttcgatgaga
     3721 tcttcttcgt ggacctgccc acggctgcgg cgcgtctcga gatcttcgcc attcattgca
     3781 agaagcgcgc ggtggcgttg accgagcccg agatgaaggc gctcgccgcg cgcagcgaag
     3841 gattctcggg ggcggaaatc gagcaggccg tggtgtccgc gctgtacacc gcgcatgcga
     3901 acaagcaggt cgtcgaggcc aagctgatcg ccgccgagct cgatgccacc aagcccctgt
     3961 ccgtggtgat gggcgagaag atcgccgaac tgcgcgcctg ggccgcggag cgcaccgtcc
     4021 cggccgatta atggtgccag gcaccgtttg ccaggaaacg gtgcctggca ccaatactta
     4081 cttgggcggc gggatcaatg atccgttgcc gtccagcttc gacagcacgt tcgcgtcccc
     4141 caggaacttg ggcaggtcga gccccgccat cgccgccacg tcatgcagcg gcggcagact
     4201 cttcaccatg ccggccagga agttggacgt ggtcgtgccg ccattgccat tagcgccggc
     4261 accggcgtcc cacaccgtga ccttgtcgat cttgatggcc ttgatggcct cgacctgcat
     4321 ggcgacgaga tgctcgagct tctcggtgag cagcaacgtg gccgccgccc gcgcatcgtt
     4381 gttggcgctg gcaaccagcg cctggtaacc ctgcgccttt gcctccagca cctggcgaat
     4441 gcccttggct tcggcttcgt acttgagcag cgtggcgtcg gccacaccct gtgcctcgcg
     4501 tcgggttttc tcggcctcgg cttccgcggc gatcgtgatc ttggtcttct cgacctgctg
     4561 acgcacgacc tcctgcgcgg tgagcctctg cagttccgcc ttggcctgcg cgcgctggat
     4621 ttcagcctcg gcatcgaact gcgccacctg gccgcgctgt gacgccaagg cggactgcac
     4681 gaacaactcc gcattcgagg ccgctatcaa cgccttcgca cggttctcgc cggagatggc
     4741 ctcggcttcc tgcgcttcta tgtagatgcg cttctccgct tcggctttct tctggccctg
     4801 gatcgaggtg gcctcgttct gcgccacctt gatctgcagg tcgcgattcg cttctgcttc
     4861 accaatcgtg gccagcgctt cctgctgctg cacgtagata cgcttgtcgg cctcggcctg
     4921 cttcttgcct ttctccgact cggcgagatg ctgtgcgacc ttgatggtgc gctcgcgaac
     4981 ctgctccgct tcgccaaccg aacccttgcg atcctggtcc gaaacgtcga ccttggcctg
     5041 gttgatggct tcggacgccg ccttcttgcc gatgctctcg atgtagcccg actcgtccgt
     5101 gatgtcggtg atgttcacgt tgatgaggta caggccgatc ttgttgagct cgggcgccac
     5161 gttgcggcgg atcgcgtcga ggaacttctc gcgatcctgg ttgatctgct cgatcgtcaa
     5221 cgaagccacc gtgagtcgca gctgaccgaa gatgatctcg cgcgccatgc tctcgacgtc
     5281 gtcctgcgcg agattgagca ggcgttccgc cgcgttgttc atgatggctt cttcggtgct
     5341 cacgcctacg gtgaacgtgc tcggcacgtt gacgcggatg ttctgcagcg acaacgcctt
     5401 ggtgagcgga atcggaatgg tgagcggcgt caggctcatg tacgtgtaat cctggaacag
     5461 cggaacgatg aacttgccgc cgccgtggac acacatcgcg gactgccctt tccccacctt
     5521 gccgaacacg accaggatct tgtccgatgg gcatcgcttg tatcgcgttg ccaggaacgc
     5581 gatcaggaaa aacaccagga ctaccaagac cactatgggc aggaatgcca tcgtgagatc
     5641 catgcgagac cctccgcgcg cgagctgcgc gcaaaattac caattgaccc gatcagcgtt
     5701 cgacgggcga gacgaccagc gtgttggccg tgacgctttg cactcgaatg gcagtgccgg
     5761 tgggcagcgt cgatccatcg gcagacaccg cctcgaactc gcgcaggcga ttggcgaagt
     5821 tgatctgtac gcggccaccg tccttgccca cggtgagata caccgagcct tcactgccga
     5881 cagcggcgaa gagactgacg gtgccactcg actgcagacg catcatcgag cggaagatct
     5941 tctggatgag ccagaccgag aacacgccgg cacccaccgc accgagtagc gcgaggaaca
     6001 acccgagcgc ggcgttgtga tacagcacga agccgaccac gccgaacatc gtgaagaacg
     6061 aggaaatgcc ctggatggtg agcgcctgga atccggcgtc ggtatccatg tgcgcggcgt
     6121 cgaggccacc gtgatggtcc agtccggcga ccgtgagaat gagtctgaag accaccacga
     6181 ttccgccgac gatgccggcg agcaggaaca actgttcgac gctgttgtat gaagcaaacc
     6241 actcagtcat ggccgcaact cccgccggat gttttgttta gttgtaccgg cagtggggac
     6301 tatagcgggg atttcaataa tctgaatcca taaaacgaaa gctgctgacc gtgattccgc
     6361 ccaggaattc cttctcgtga tatgccagtt ccgtcatgtc gtcgccggcg gcgcccacgg
     6421 cgaccatcag tggcagcagg tgttcctcgc gtggatgcgc agcgcgcgct gagggcgccg
     6481 tttcccaggc ggtcagtagt ttgccgcggt cggccggact gccgcccagc agcgcgccaa
     6541 tcagccaggc gtcgaattga cgcgacgggc cgtaagccgc cgggctgaag aactggcgca
     6601 ggttgtggta gctcaatcca ctgccgacga tcaacacgcc ttcatcgcgc aacggcgcga
     6661 gcgcgcggcc catggcgaga tgcgtcgccg gatccagccc ccgcttgagc gacaactgca
     6721 ccaccggcac atcggcgtcc gggtagatga ctttcagcgg agtgaacgcg ccatggtcga
     6781 acccgcgttc cgcgtcgagc cgcgcgggga ggcccgccgc gtcgagcatc gacttcacgc
     6841 gcgccgccaa caccgggtct cccggcgcgt cgtaatgaat gtgataggtg tagtcgggaa
     6901 aaccgccgta gtcgtaaatc atcggtggct tgggattcga cgtcagcgtg aactcggact
     6961 cttcccaatg cgccgagatc atgagcactg cggttggctt tccgccgatc tgcctcggca
     7021 tgtcggccag agccgcttcg ag
//
LOCUS       DASQJS010000413         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4662074, whole genome shotgun sequence.
ACCESSION   DASQJS010000413 DASQJS010000000
VERSION     DASQJS010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4662074"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VEW08_16310"
     CDS             complement(<1..462)
                     /locus_tag="VEW08_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42357.1"
                     /translation="MPRFLRRHVLAVLTICALASFPAGAQPSSAFRDNPAWTFADGAL
                     TTQSEAGKTPLVSRLTLADSVTAFDFRAPAGARGTLYVQGRYAFELVGNGEWQHFELR
                     FRAPRFDAGYNKLDNAFVLDVRNGTDFRRNAIVEGPSEGAYWNGEDRRGPSF"
     gene            527..>2143
                     /locus_tag="VEW08_16315"
     CDS             527..>2143
                     /locus_tag="VEW08_16315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ42358.1"
                     /translation="MLFAANGGVALPAPQPEIIVTGSRVATLPDDIAANVTVLKREEF
                     DVEKPAKLADVLQRVAGVHVDQVGGRGGTGSVYVRGADPNYTLVLVDGVRVNDPTNAR
                     GGSFDLSALDMADVERVEIARGPYSAVYGGDALAGVINIVTRREPPAKGYASIDAMGG
                     AYDTREISLQTGGPGAAGNWNMGFSDTREGELVRGNQFASQRFSGNFDTEFGASTTLS
                     VSGRYSETEREGFPDDSGGYEFAEIRDVEKREAEEGVFGVGLTHRAGAATFGLTLGYI
                     DRNDHIDSPGIAPGIRDPFGVPPSIVDTNLERLTATFTGTQKFSELVTLAYGAEWQRE
                     EGTSDGSLDFGGGFILPTSFELTRTSWAPFAEVRLDSSFGLSTQAGVRVDNPDGYSSV
                     TSPRVRVAYEFADSGFTVAGAWGKAFKLPSLYALGHPLVGNPDLVPERGESYELELSQ
                     QSEDGAARWSATYFEGEFLNAIDFDSGPPPMLVNRNRVDSEGVELAGRLGVGEQWMLD
                     ASVTNAKSEIASTGGQLRNRPEWRAGFGAHW"
BASE COUNT          367 a          661 c          739 g          376 t
ORIGIN      
        1 gaacgagggt ccgcggcggt cttcaccgtt ccagtacgct ccctcgctgg gtccttccac
       61 gatggcatta cgacgaaagt cggtaccgtt gcgcacgtcg aggacgaagg cgttgtcgag
      121 cttgttgtag cccgcatcga agcgcggggc gcggaaccgc agctcgaagt gctgccattc
      181 gccgttgccc acgagttcga aggcgtaccg gccctggacg tatagcgtgc cgcgcgcacc
      241 ggcgggtgca cggaaatcga aggccgtgac gctatcggcg agcgtcaggc gcgaaaccag
      301 gggtgttttg ccggcttcgg attgcgtcgt caacgcgccg tcggcaaagg tccacgcggg
      361 gttgtcccga aaggcggagc ttggctgcgc cccggccggg aaacttgcga gcgcacatat
      421 ggtcagtacc gccaggacat ggcggcgcag aaatctcggc atggaacccc cttgtgtgcg
      481 gctacactaa cgcaatcccc acgaacgaga aacggccttg atctggatgc tttttgccgc
      541 caacggcggc gttgccctgc ccgcgcccca acccgaaatc attgtcaccg gctcgcgcgt
      601 tgccacgctg ccggatgaca tcgcagccaa cgtcaccgtg ctcaagcgcg aagaattcga
      661 tgtggaaaaa cccgccaagc tggcggatgt gctccagcgc gtggccggag tgcacgtgga
      721 ccaggtgggc ggccgcggcg gtacgggctc ggtatatgtt cgcggtgcgg accctaacta
      781 cacgctggtg ctggtggatg gcgtgcgcgt caacgacccc accaatgcgc gtggcggctc
      841 gttcgacctg tcggcgctcg acatggcgga cgtggagcgc gtggagattg cgcgcggccc
      901 atattcggcc gtgtatggcg gcgatgcgct ggcgggcgtc atcaacatcg tcacgcggcg
      961 tgaaccaccg gccaagggtt atgcgtccat cgatgccatg ggcggcgcgt acgacacgcg
     1021 cgagatctcg ctgcaaactg gcgggccggg agcggccggc aactggaaca tgggtttcag
     1081 cgacacgcgc gaaggcgagc tggtgcgtgg caatcaattt gcctcgcagc ggttttcggg
     1141 aaacttcgac accgagttcg gtgcgtccac cacgctgagc gtgtcggggc gatattcgga
     1201 aaccgagaga gaaggttttc ccgatgacag cggcggctac gaattcgcgg aaattcgcga
     1261 cgtggaaaag cgcgaggcgg aggaaggcgt gtttggcgtg ggcctcacgc atcgcgccgg
     1321 cgctgccacc tttggcctga cgctgggtta catcgaccgc aacgaccaca tcgactcgcc
     1381 gggcatcgcg cccggcattc gcgatccgtt cggcgttccg ccgagcatcg tggatacgaa
     1441 cctcgagcgc ctcacggcca cgttcacggg aacgcagaag ttttcggagc tggtcaccct
     1501 cgcctatggc gcggagtggc agcgtgagga aggcacgagc gacggctcgc tggatttcgg
     1561 cggcggcttc atccttccca cctcgttcga gctcacgcgc acgtcgtggg cgccgtttgc
     1621 cgaggtgcgc ctggattcga gtttcggtct gtcgacgcag gctggagtgc gcgtggacaa
     1681 cccggatggg tattcctccg tgacgagccc gcgcgtgcgc gtggcttacg aattcgcgga
     1741 cagcgggttc acggtggccg gcgcctgggg caaggccttc aaactaccca gcctctatgc
     1801 gctgggccat ccgctcgtgg gcaacccgga cctcgtgccc gagcgcggtg agtcctatga
     1861 actcgagttg tcgcagcagt cggaagacgg cgcggcgcgc tggagcgcca cctatttcga
     1921 aggcgagttc ctcaacgcca tcgacttcga ttccggcccg ccgccgatgc tggtgaatcg
     1981 caatcgcgtg gattcggagg gtgtcgaact cgccgggcgt ctgggcgtcg gcgagcagtg
     2041 gatgctcgac gccagcgtca cgaatgcgaa gagcgagatt gcttccaccg gtgggcagct
     2101 gcgcaatcgt cccgagtggc gcgcgggctt cggcgcgcac tgg
//
LOCUS       DASQJS010000414         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4348523, whole genome shotgun sequence.
ACCESSION   DASQJS010000414 DASQJS010000000
VERSION     DASQJS010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4348523"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1699
                     /locus_tag="VEW08_16320"
     CDS             <1..1699
                     /locus_tag="VEW08_16320"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HYJ42359.1"
                     /translation="EPIGREMLHATLWMQTAPEYRASVLQVYRLAGERIAAPAPGSAA
                     IEQSQVAAADLARLPTAVVLDLDETVLDNTVYQARLMRDHTVYNAKGWGEWVAAGEAE
                     AVPGAREFIAKARALGHTVFYISNRDCTAPPPTAADPCPAKTATMRNLVALGIDEAPD
                     PARLLLRGERQEWNKSNKTTRRAFIAAKYRIVALVGDDLGDFIDPLVFAGDRERLEPR
                     FGQGWFVLPNPIYGSWTRHFNTIDEKFAALHTSDVVLELPGDGRWKGNETSVRIASWN
                     VEYLMTRATHLALRDDCAQNGGMVGGDARTLPCAITRRAPRTPEDYTSLRSYAAQLRA
                     DVVALQEVDGPDAAEQVFPGYDFCFSSRAHTQKNGFAIRRGLPHRCEPEYEALSLNNA
                     VRRGVVVTFFPGTDNEFRLMSVHLKSGCPAGPLTAPGRNCELLSRQVEPLEAWIEGEA
                     NAGHRFGLLGDFNRRFSIEKGPARDANGRQLNVYAEINDGIPAASKLTNITGAEKFTP
                     CTTDSEYREFIDTILLGRDLAKSVVKKSFVRVVFNDQDAKAHWLSDHCPVGIELRIR"
     gene            complement(1626..2855)
                     /locus_tag="VEW08_16325"
     CDS             complement(1626..2855)
                     /locus_tag="VEW08_16325"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYJ42360.1"
                     /translation="MRSVLALLIFAAVGIAPAAAADRPSGLIRIATFNCSLNRGSDGA
                     LRRDLATPGNAQAAAVAEIIQRVRPDILLLQEFDYDADGAALRDFQANYLGRPQNGSP
                     AIRFAHSFFTESNTGVPSGLDLGNDGTVHGGEDALGFGEFPGQYAMALLSRFPIDQAH
                     ARTFRRFLWRDMPGALLPPGWYSPEELAVLPLSSKSHWDVPVRIGKQTLHLLASHPTP
                     PAFDGPEDRNGRRNHDEIRFWSDYLDGAAYIRDDAGRRGGFRGKAFLIMGDQNSDPVD
                     GGSLNDAIGTLLAHPRVDSRFVPQSPGGAEASAAQGGANAAQRGDPRSDTADFNDRVA
                     GNLRVDYLLPSKGMPVCAGGVFWPSQTDPAASLVWGDRPPPSSDHRLVWIDVSVSGAR
                     CPPGSDPTTSAPSHPGH"
     gene            complement(2870..>3439)
                     /locus_tag="VEW08_16330"
     CDS             complement(2870..>3439)
                     /locus_tag="VEW08_16330"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ42361.1"
                     /translation="TNIASLALYQIDYTNRIVSTFNADLGYNEDRNVGDAEIVGMDAQ
                     IGHRFGELVELTASASYNDSELTGSLNPALDGKKLVETPEWTYSARMDINVTEDLHFG
                     LQAKQVDDRFSTDLNDEIAPGYTVVDIDLNYGFKLPGFERAELQLNVTNLLDEEYFGN
                     ISSGTGGNSVGFFSIGAPRTWVASVRFDF"
BASE COUNT          616 a         1110 c         1108 g          605 t
ORIGIN      
        1 cgagccgatc ggacgcgaaa tgctccacgc cactctgtgg atgcagaccg cgcccgaata
       61 tcgcgccagc gtcctgcagg tctaccggct cgccggggag agaatcgccg ctcccgcgcc
      121 cggcagcgcc gcaatcgagc aatcccaggt agcggccgcg gacctcgcac gcctgcccac
      181 ggcggtcgtg ctcgatctcg atgaaaccgt actcgacaac acggtgtatc aggcgcggct
      241 gatgcgcgat cacactgtgt acaacgcgaa aggctggggc gaatgggttg cggccggcga
      301 ggcggaagcg gtgccgggcg cgcgcgagtt catcgccaaa gcccgcgctc tcggccacac
      361 ggttttctac atcagcaatc gtgactgcac ggcgccaccg ccaactgctg ccgatccgtg
      421 cccggccaag acggccacca tgagaaacct ggttgcactc ggcatcgatg aagcgccgga
      481 tccggcccgc ctgctgctgc gcggcgaaag acaggaatgg aacaagagca acaagaccac
      541 ccggcgcgca ttcattgccg ccaaataccg catcgttgcg ctggtggggg acgatctcgg
      601 cgatttcatc gatccgctcg tctttgccgg cgaccgtgaa cggctcgaac cacgtttcgg
      661 ccagggctgg ttcgtcttgc cgaatcccat ctacggctcc tggacccggc acttcaatac
      721 tatcgatgag aagtttgccg ccttgcatac ctccgatgtc gtgctcgagc tgccgggcga
      781 tggtcgatgg aagggcaatg aaacaagcgt gcgaatcgcg agctggaatg tcgaatacct
      841 gatgacgcgg gccactcacc tcgcgctgcg cgacgactgc gcacagaacg gcggcatggt
      901 cggcggcgac gcgcgcacgc tgccctgcgc catcacccgg cgcgcaccgc gcactcccga
      961 ggactacacg agcctgcgca gttatgccgc gcagctgcgc gccgacgtgg tcgcgctaca
     1021 ggaggtcgat ggtccggatg ccgccgaaca ggtgtttcca ggctacgact tctgcttcag
     1081 ttcgcgcgcg cacacgcaga agaacggctt cgcgattcgc cgcggtttgc cgcaccgctg
     1141 cgaaccggag tacgaggccc tgtcgttgaa caacgccgtg cgtcgcggtg tcgtcgtcac
     1201 gttcttcccg ggtaccgaca acgaattccg tctcatgtcg gtgcacctga agtcgggctg
     1261 tcccgctggt cccctgaccg cgcccgggcg caattgcgaa ttgctctcgc gccaggtgga
     1321 gccgctcgaa gcctggatcg agggtgaagc caatgccggc catcgtttcg gtttgttagg
     1381 cgacttcaat cgaaggtttt ccatcgagaa gggcccggcg cgtgatgcca atggccgcca
     1441 gctcaacgtc tacgccgaaa tcaacgacgg gattccggcg gcctcgaaac tgacgaacat
     1501 cacgggcgcc gagaagttca cgccatgcac gaccgacagc gaatatcgcg agttcatcga
     1561 caccatcctg ctgggccggg atctcgcgaa aagcgtcgtg aagaaatcct tcgtgcgcgt
     1621 ggtcttcaat gaccaggatg cgaaggcgca ctggttgtcg gatcactgcc cggtgggcat
     1681 cgagctccgg atacgctgac gtcgatccac acgagccgat ggtccgaact tggcggcggg
     1741 cggtcgcccc acaccaatga cgcggccggg tcggtctgcg acggccagaa cacgccgccc
     1801 gcgcacaccg gcatgccttt cgaaggcagt agatagtcga cgcgcaggtt gccggccacc
     1861 cggtcgttga aatcggcggt gtcggagcgc ggatcgccgc gctgcgcggc gttggcgccc
     1921 ccctgcgcgg cgctggcttc ggcaccgcct gggctctgcg gcacaaaacg cgagtcgacg
     1981 cgcggatgcg ccagcagtgt gccgattgcg tcgttcaggc tgccaccgtc caccgggtcg
     2041 gagttctggt cgcccatgat caggaacgcc ttgccgcgaa agccgccgcg ccgacccgcg
     2101 tcatcgcgga tgtaggccgc gccatccagg tagtcgctcc agaaacggat ttcgtcgtga
     2161 ttgcgcctgc cattgcgatc ttccggtccg tcgaatgccg gcggtgtcgg atgactcgcg
     2221 agcaggtgca gagtctgttt tccgatgcgc actggcacgt cccaatggct cttggaagat
     2281 agcggcagca ccgccagttc ctctggcgaa taccagcccg gaggcaacag cgcgcccggc
     2341 atgtcgcgcc acagaaacct gcggaaggtt cgcgcgtgcg cctggtcgat gggaaatcgc
     2401 gatagcagcg ccatggcgta ctggcccggg aattcgccga agcccagcgc gtcttcgccg
     2461 ccgtgaacgg tgccgtcgtt gcccaggtcg aggccggaag gcacgccggt attggactcg
     2521 gtgaaaaagg aatgcgcgaa ccggatggcc ggcgagccgt tctgtggcct gcccaggtag
     2581 ttagcctgaa agtcgcgcag cgccgcgccg tcggcgtcgt agtcgaattc ctgcagcagc
     2641 agtatgtccg gccgcacgcg ctggatgatc tccgccaccg cggctgcctg cgcatttccg
     2701 ggcgtggcca gatcgcggcg cagggcgccg tcgctacccc gattgaggct gcaattgaaa
     2761 gtggcgatgc ggatcaggcc ggacggccgg tccgccgcgg cggcgggcgc aatgcccacc
     2821 gccgcgaaga tcagtaacgc gaggacgctg cgcacgtccg cgcgtgtgtt cagaagtcga
     2881 agcggaccga cgccacccac gtgcgcggtg cgccgatgga gaagaagccc accgagttgc
     2941 cgcccgtgcc cgagctgatg ttgccgaaat actcttcgtc tagcaggttg gtcacgttca
     3001 gctgcaactc ggcgcgttcg aaccccggca gcttgaagcc gtagttcagg tcgatgtcga
     3061 ccacggtgta gcccggcgca atttcgtcgt tgaggtcggt ggagaaccgg tcgtccacct
     3121 gcttcgcctg gaggccgaaa tgcaggtctt cagtcacgtt gatatccatg cgcgcggaat
     3181 aggtccattc cggcgtttcc accagcttct tgccgtcgag cgccgggttc aggctgcccg
     3241 tgagctcgct gtcgttgtat gacgccgagg ccgtgagctc caccagctcg ccgaatcgat
     3301 gtccgatttg tgcatccatt cccacgattt ccgcgtcacc cacattgcgg tcttcgttgt
     3361 agccaaggtc agcattgaac gtagagacga tgcgattggt gtaatcgatt tgatagagcg
     3421 cgagcgaggc gatgttggt
//
LOCUS       DASQJS010000415         4921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2152642, whole genome shotgun sequence.
ACCESSION   DASQJS010000415 DASQJS010000000
VERSION     DASQJS010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2152642"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VEW08_16335"
     CDS             <1..606
                     /locus_tag="VEW08_16335"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYJ42362.1"
                     /translation="CAREEERQGIDREEALMHAGRVRLRPILMTTFALMAGMMPVAIG
                     VGEGGEFYSPMAVAIIGGTVVSTLLTLLVVPSFYDSIEIARERAGHKFRARAAYLNPI
                     VAFLLTFGEVLATLVFFRFFFRSIRSVIGLATPAEHPVERAERLVGLAPPPGFIARPK
                     RWQHSGFRRDDTYIGATPVSPDPGTTAEPKPKPRWKPIKAS"
     gene            complement(656..3685)
                     /locus_tag="VEW08_16340"
     CDS             complement(656..3685)
                     /locus_tag="VEW08_16340"
                     /inference="COORDINATES: protein motif:HMM:NF024686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HYJ42363.1"
                     /translation="MKNFAAAARLGVLVLFATGLLTGCKKADAPVFSPAPGTYTGTQY
                     IAITSQTFGATIIYTTDGTAPSCQKERGTIYSEPVAVAADTTLKAMACALLRAESLIS
                     TGVYVIKPPETVATPVFTPAAGTYITTQSVSITSATTDASIHYTSDGTPATCASGTVF
                     AGPIEVAQSVTLSAIGCATGKIDSAVATAAYVITPPAAPPVFDPAPGEYTSAQQVTLT
                     SATPAATFRYTTNGSEPSCSTGSTYGGPVAIANSLTLKAVACAAGFSDSLVTSGGYDI
                     TLPPEESVWKSIDFNNGANTAQGTHSTNEDGNALNISSRGKVESGQQVFHFVYANVTG
                     DFVMTARLDGFDFGGLVSNQGRTGLLLTPDLTATTNNLVYASSTLAATSPVTFLRTHR
                     LAAGTTNSNSTIATNGTGVRYLKLTRTGDTYQAAASADGGVNYVSSAVQTFAAGLPPS
                     VFVGFMISSSSNTASASATFSDVHIRDSAGNEIIGPNEFTGDIGSGTTGGGGGGPIPP
                     TGPTQPEDLSRGATPAEPALVGNAPRYNLEGYAAGPVTGGGNIPDTDSRYRKVTTATE
                     LVAALKDAKASGPNPVKVIEVMNDLNLGWNEVGTLLQAQASNPLRPQNSPAKKHPALI
                     ASGVTLLDIQAFNGLTIFSRNGATIRHAEFNIKGGTNLILRNLRFDELWEWDEATKGA
                     YDSNDWDFVTIGDGGGITSGIWVDHCTFTKAYDGVLDIKKGASAITVSWSEVVPAAAG
                     PGSFVQRQFDDLETNRTSNAMYNVLRGAFTPQQIVDIALPQKKGHLIGSTDMEGISSY
                     TVTLHHNYYQDLQDRMPRLRGGDVQVYNLYVDSSNARLVKVMRDGIVAGNATLAAQLS
                     STYSFGVTSQASISTEGGAVEVLNSVFFGVLTPFRNNQTDVTNPAYTGAIRGFGNKHI
                     LLTTDTTYMPASSQQNPFMDRGFEWASWTGDSDAVGSTFGPTQAPQVPFEWNNATPTY
                     PMTLDSLEVLPGLLTGPQGAGAGKIGMTTQQWLQVTN"
     gene            complement(3820..>4921)
                     /locus_tag="VEW08_16345"
     CDS             complement(3820..>4921)
                     /locus_tag="VEW08_16345"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HYJ42364.1"
                     /translation="VSVFVDGERRFNAGTIADFFSTQSGIFALGVNYWDLPFNGMIDE
                     LKFYEAVLSAQEIRALDIDHLSNPELLASAATLLDLGDLTAVRQDLTLPRSGAYASAI
                     SWASSNPAVLSDRGRVTRPGRDQPDVDVTLTATVSLAGQVTSRSFAVRVKSLAPPVPV
                     AVYRFEDNLNEASGARAPGAPTGSRVFESGGSVSYVNGAVGRALQLNGSTGVRLPDNL
                     IRDRSYSISMWLNPTVTTQFTTAFFGWATDSSWISVVPRGPGGAQHTMLWSGTQWFDG
                     TFNSSIPTGAWSHLVMVVNNGTLSLYLNGALVNTMANFPDVFTPAPTTQFALGVNFWD
                     TPYNGLVDELKLYDEAIALENVQELFGERSGP"
BASE COUNT          822 a         1496 c         1659 g          944 t
ORIGIN      
        1 tgcgcgcgcg aagaagaacg gcagggcatc gatcgcgaag aagcgctcat gcatgccggc
       61 cgcgtgcgtc tgcgcccgat cctcatgacg acgtttgcgt tgatggccgg catgatgcca
      121 gtggccatcg gtgtcggaga aggcggtgag ttctactcac ccatggcggt ggcgatcatc
      181 ggcggcaccg tggtgtcgac gttgctgacg ctgttggtcg tgcctagctt ttacgacagc
      241 atcgagatcg cacgcgagcg tgcgggccac aagttccgcg cgcgggcggc gtacctcaat
      301 ccgatcgtgg cgttcctgct cacgttcggc gaagttctgg caaccctcgt gttcttccgc
      361 ttcttctttc gctcgatccg ttctgtgatc ggtctcgcca caccggcgga acatcccgtc
      421 gagcgagccg agcggctggt cgggctggcg ccgccgcctg gtttcatcgc gcgccccaag
      481 cgctggcagc acagtgggtt ccggcgcgat gacacgtaca tcggcgcgac gccggtgtcc
      541 cctgatccag ggacaacggc tgaacccaag ccgaaaccgc gctggaagcc aatcaaggcc
      601 agctgataaa cgcggcaaag ccgggagcga ctcccggctt tgccgatcga tcacttcaat
      661 tggtgacctg cagccactgc tgcgtggtca tgccgatctt gcccgctccc gcaccctggg
      721 ggccggtgag cagacccggc agaacttcca gcgaatcgag cgtcatcggg taggtgggcg
      781 tggcgttgtt ccactcgaac ggcacctgag gcgcctgcgt gggtccgaac gtgctgccca
      841 ctgcgtcgct gtctccggtc cagctcgccc actcgaaccc gcgatccata aacggattct
      901 gctgcgagct tgccggcatg taggtggtgt cagtggtcag caggatgtgc ttgttgccga
      961 atccgcggat cgcacccgtg taagcgggat tcgtcacgtc ggtctggttg ttgcggaacg
     1021 gcgtgagcac accaaaaaat acactgttca ggacctcgac cgcaccgccc tcggtggaga
     1081 tcgaggcctg cgaggtgacg ccgaagctgt aggtggagct cagctgcgcg gcgagcgtcg
     1141 cgttgcccgc cacgatgccg tcacgcatca ccttcacgag cctcgcattc gagctgtcga
     1201 catacaggtt gtacacctgc acatcgccgc cccggagccg cggcatgcgg tcctgcagat
     1261 cctggtagta gttgtgatgc agcgtgaccg tgtaagacga gatgccttcc atgtcggttg
     1321 agccgatcag gtgacccttc ttctgcggca gcgcgatgtc gacgatctgc tgtggagtga
     1381 acgcgccgcg cagcacgttg tacatcgcgt tgctggtgcg attggtctcg agatcgtcga
     1441 actgccgctg cacgaagctg cccgggccgg ccgccgcggg caccacttcg ctccacgaca
     1501 cggtgatcgc gctcgcgcct ttcttgatgt cgagcacgcc gtcataggcc ttggtgaacg
     1561 tgcagtgatc gacccagatg ccggaggtga tgccgccgcc atcaccaatc gtgacgaagt
     1621 cccagtcgtt ggagtcgtat gcgcccttcg tggcttcgtc ccactcccac agttcatcga
     1681 acctgagatt gcgcaggatg aggttggtgc cgcccttgat gttgaactcg gcgtggcgga
     1741 tcgtcgcgcc attgcgcgag aagatcgtga ggccattgaa ggcctggatg tcgagcagcg
     1801 tcacgccgct cgcgatcagc gcgggatgtt tcttcgccgg gctgttttgt ggtcgcaggg
     1861 gattgctcgc ctgcgcctgg agcagcgtgc ccacttcgtt ccagcccagg ttgaggtcgt
     1921 tcatgacctc gatgaccttc acggggttgg ggcccgacgc cttcgcatct ttgagcgctg
     1981 ccaccagctc ggtggccgtg gtgaccttgc gatagcgcga gtccgtgtcg ggaatgttgc
     2041 cgccgcccgt gaccggaccc gcggcatatc cctcgaggtt gtaacgcggt gcatttccta
     2101 ccagcgccgg ttcagccggc gtcgcgccgc gcgaaagatc ttcgggttgt gtcgggccgg
     2161 tgggcggaat cggaccgcca ccgccgccac cggttgtgcc ggagccgatg tcgccggtga
     2221 actcattggg gccgatgatc tcgttgcctg ccgaatcgcg aatgtgcaca tcgctgaaag
     2281 tggccgaggc gctcgccgta ttgctgctcg agctgatcat aaacccgacg aacaccgacg
     2341 ggggcaggcc ggcagcgaag gtctggaccg cggagctcac gtagttcacc cctccatccg
     2401 ccgatgcggc ggcctggtag gtatcgccgg tgcgcgtcag cttcaggtaa cgcacgccgg
     2461 tgccgttcgt ggcgatcgtg ctgtttgaat tggtggtgcc ggcagcgagg cggtgggtcc
     2521 tgaggaaagt gacaggagaa gttgccgcca acgtggagga cgcatacacc aggttgttgg
     2581 tcgtggcagt caggtccggg gtcaacaata atccggtgcg cccctggttg ctgaccaggc
     2641 cgccgaagtc gaagccatcg agccgcgccg tcatcacgaa atcgccggtg acgttggcgt
     2701 agacgaagtg gaacacctgc tgtccgctct caaccttgcc gcgggacgaa atgttcagcg
     2761 cattgccgtc ctcgttggtg ctgtgcgtgc cctgcgcggt gttggcgcca ttgttgaaat
     2821 cgatgctctt ccacaccgac tcttctggcg gcagagtgat gtcgtacccg ccgctggtga
     2881 cgaggctgtc ggagaagccg gcggcgcagg ccaccgcttt cagggtcagg gaattcgcga
     2941 tcgcaaccgg cccgccgtag gtagagcccg tggagcagct cggttccgaa ccgttagtgg
     3001 tgtagcggaa tgtggcggcg ggagtcgccg acgtgagcgt gacctgttgg gcgctggtgt
     3061 attcgccggg cgccggatcg aacaccggtg gcgccgcggg cggagtgatg acatacgcgg
     3121 cggtggcgac ggcgctgtcg atcttgccgg tcgcgcagcc aatcgcgctc agggtgacgg
     3181 attgcgccac ttcgatgggg ccggcgaaca cggtgccgct ggcgcaggtc gcgggcgtgc
     3241 cgtcgctggt gtagtgaatg ctggcatcgg tcgtcgcgct ggtgatgctg acagactgag
     3301 tcgtgatgta cgtgcctgcc gccggggtga acaccggcgt cgccaccgtt tccggcggct
     3361 tgatgacgta aacgccggtg gaaatgagac tctcggcgcg cagcagcgca caggccatgg
     3421 ccttgagcgt ggtgtcagcg gcgaccgcca ccggttcgga gtagatagtg ccgcgttcct
     3481 tctggcagct cggtgccgtt ccgtcggtgg tgtagatgat ggtggcgccg aacgtctgcg
     3541 acgtgatcgc gatgtattga gtgcccgtgt aggtgccggg tgccggactg aagacaggcg
     3601 cgtccgcctt cttgcagccc gtgagcaagc cggtggcgaa aagcacgagc acgccgaggc
     3661 gcgccgcggc agcgaaattc ttcatctggt tcccctcgtc attgttgtag gtcatagccc
     3721 ggtgatcttg gccaccggtg tcatgccgca tgctaccggt gtcatcctga gacggaccgg
     3781 ccggcgcgtc aactcgccag ttttacttaa gggggctttt cacgggccgg aacgttcgcc
     3841 gaacaactcc tgcacgttct cgagggcgat ggcttcgtcg tacaacttga gttcgtcgac
     3901 gagtccgttg tacggcgtgt cccagaaatt cacgcccaac gcgaactgcg ttgtgggcgc
     3961 cggcgtgaac acgtcgggaa aattggccat ggtgtttacc agcgcgccat tcagataaag
     4021 actgagcgtg ccattgttga ccaccatgac caggtgcgac caggcgccgg ttgggatgga
     4081 ggaattgaag gtgccgtcga accattgggt gcccgaccac aacatggtgt gctgtgcgcc
     4141 accgggtccg cgcggcacca cgctgatcca gctcgaatcg gtggcccagc cgaagaacgc
     4201 ggtcgtgaac tgcgtggtca cggtcggatt cagccacatc gagattgagt agctgcggtc
     4261 gcggatgagg ttgtcaggca ggcgcacgcc ggtgctgccg ttcagctgca gcgcgcggcc
     4321 cacggcgcca ttcacataag agacgctgcc accgctctcg aatactcggc tgccggtcgg
     4381 cgctccaggt gcgcgtgcgc ccgaggcttc gttgagattg tcctcgaagc gatagacggc
     4441 cacgggcacc ggcggcgcca gcgacttcac gcgaaccgcg aaactgcgcg atgtgacctg
     4501 cccggcgagc gaaacggtgg cggtgagtgt gacgtccacg tccggctgat cgcggccggg
     4561 acgggtgacg cggccacgat cgctgagcac cgcgggattg gacgatgccc agctgatggc
     4621 gctggcatag gcgccgctgc gcggcagcgt gagatcctgg cgcactgcgg tcaggtcgcc
     4681 aagatcgagc agcgttgccg ccgacgccag cagctccgga ttggagaggt ggtcgatatc
     4741 gagagcccgg atctcctggg cgctcaacac cgcctcatag aacttgagct cgtcgatcat
     4801 gccgttgaag ggcagatccc agtagttgac gcccagcgca aagatgccgg actgggtgct
     4861 gaaaaagtcc gcgatggtgc ccgcgttgaa acgccgctcg ccgtcgacga acaccgacac
     4921 c
//
LOCUS       DASQJS010000416         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3415752, whole genome shotgun sequence.
ACCESSION   DASQJS010000416 DASQJS010000000
VERSION     DASQJS010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3415752"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VEW08_16350"
     CDS             <1..810
                     /locus_tag="VEW08_16350"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HYJ42365.1"
                     /translation="PQFGRLTLRITDSPVTEAERVVVQFTGLEIKPRAAADPEVFDFD
                     APRQIDLLALDGGGSEILLSDELLPAGEYEWIRLKVNAGRDASDSFIELADGSRHPLF
                     IPSGNQTGLKLIRGFVIGVGSSHNFTIDFDLRKSVIHPPGLGDPYLLKPVLRLVDNLE
                     VGAIDGTVAQGLVVDGCAPSVYLYSGADGLPDDIGSATPPLASTAVNLDNTTGVYRFK
                     FAFMPAGANTIAFTCAADEDAADVDDVIAFSAPINVTVTAGTTITVDVPAP"
     gene            877..1464
                     /locus_tag="VEW08_16355"
     CDS             877..1464
                     /locus_tag="VEW08_16355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYJ42366.1"
                     /translation="MNDSSAADFDRLLRGHVRPLYRYAYRWTGDVDQAEDLVQETLTR
                     LYPELHRLREIEQLRPWVARVMYRIFVDWLRRANRSPVTFVGRSGSTGEFLEDDEEDD
                     QAPDESWNPEALTERAFERERIEAAWTHLHEQHRVVLSLHEIEGYSLEEVAHIIEAPL
                     GTAKSRLNRARNRLRELLTEGTNSSAPTCNHSETS"
     gene            1474..2019
                     /locus_tag="VEW08_16360"
     CDS             1474..2019
                     /locus_tag="VEW08_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42367.1"
                     /translation="MVDDLQKLRAGLSAMPVPEPRPGFVDRVLANASGGKAPEVRGIR
                     AAIRRPYTWWAAGTGALAASLAWMALFFFQSGAVTDTRLVLALNESRDVPLVIDAERN
                     LDGATIRLSVSGSIGLTGYGEQHEIEWTTSLTQGANLLSLPVFARSQGDGLVVAEIEH
                     EGRTRRLSMALHVVAPEDDSA"
     gene            2016..2435
                     /locus_tag="VEW08_16365"
     CDS             2016..2435
                     /locus_tag="VEW08_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42368.1"
                     /translation="MSSKGIMRRARRLYIASLLALITPAVFAQSPPARNRDDLDVTMH
                     VIVDPDAKVPDEIVRRIPLPKPAQPAPGATSTSTTESDKPKGPDGKGRDFGQQVSEDA
                     RQRSEEARNNKKTKPPPPGQPPGRPPSPPGRPPTPGG"
     gene            2476..>2657
                     /locus_tag="VEW08_16370"
     CDS             2476..>2657
                     /locus_tag="VEW08_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42369.1"
                     /translation="MLFSGGAHAAAGDAKARFAEARTAFEAGDFSRALFLYEQCLALG
                     MQGPAVHYNIGVAAYRA"
BASE COUNT          470 a          872 c          858 g          457 t
ORIGIN      
        1 ccgcaattcg ggcgcctcac gttgcgaatc accgactcgc cggtgaccga ggccgagcgt
       61 gtggtcgtgc agttcaccgg cctcgaaatc aagccacggg cggccgccga tcccgaggtg
      121 ttcgatttcg atgcgccgcg gcagatcgac ctgctggcgc tggacggcgg tggcagcgaa
      181 atcctgctgt ccgacgaatt gcttccggcc ggcgagtacg agtggatccg gctgaaggtc
      241 aacgcgggcc gcgatgcctc ggactcgttc atcgagctgg cagacggttc gcgtcacccg
      301 ctgttcatcc ccagcggcaa ccagaccggg ctcaagctca ttcgtggctt cgtcatcggc
      361 gtcgggagct cgcacaactt caccatcgat ttcgacctgc gcaagtccgt cattcatcca
      421 ccggggctgg gcgatccgta cctgctcaag ccggtgctgc gcctggtcga caacctggaa
      481 gtcggcgcca tcgatggcac cgtcgcgcag ggactggtcg tcgatggctg cgctccgagc
      541 gtctatctat acagcggtgc cgacgggctg ccggacgata taggcagcgc cacaccgccg
      601 ctcgccagca ccgccgtgaa tctcgacaac acgaccggtg tgtatcgctt caagtttgca
      661 ttcatgcccg ccggtgcgaa cacgattgcc ttcacctgcg cggcagacga ggacgccgcc
      721 gacgtcgacg atgtcatcgc attctccgcg ccgatcaatg tgaccgttac ggccggaaca
      781 acaatcacgg tggacgtccc tgcgccgtga cgcaccccgg gtagcagcga gcgtatggac
      841 tcgtcgcgcc cggggtgcga taactttccg tcttagatga atgattcctc tgctgcggat
      901 ttcgaccggt tgctgcgcgg gcacgtgcgc ccgctctatc gctacgccta tcgctggacg
      961 ggcgacgtcg accaggccga ggacctcgtg caggagaccc tgacgcggct gtatccggag
     1021 ctgcaccggc tgcgcgagat cgaacaactg cggccgtggg tggcgcgcgt catgtaccgg
     1081 atattcgtgg actggctgcg ccgcgcgaat cgctcgccgg tcacgttcgt gggccgcagc
     1141 ggctcgacgg gcgaatttct cgaggacgac gaagaggacg accaggctcc cgacgagtcg
     1201 tggaatcccg aggccctcac cgagcgggcc ttcgaacgcg agcgaatcga ggcggcgtgg
     1261 acccacctgc acgagcagca ccgcgtggtg ttgtcgctgc atgaaattga ggggtacagt
     1321 ctcgaagaag tggcgcacat catcgaagct ccgctcggaa ccgccaagtc acgcctcaac
     1381 cgcgcccgca atcgcctgcg cgaattgctg accgagggaa cgaattcgtc cgctccgacg
     1441 tgtaaccatt cagagacatc atgagactgc aacgtggttg acgatcttca gaaattgcgc
     1501 gcgggtctga gcgcgatgcc agtgccggaa ccccggcccg gtttcgtcga ccgcgtgctg
     1561 gcgaacgcgt cgggtggcaa ggcgcccgag gtgcgcggca ttcgcgctgc gattcgccgg
     1621 ccgtacacct ggtgggccgc gggcacgggt gcgctcgccg cgtcgctggc gtggatggcg
     1681 ctgttcttct tccagtccgg cgccgtgacc gacacacgcc tggtcctcgc gctcaatgag
     1741 agccgcgatg taccgctggt catcgatgcg gaacgcaatc tcgacggtgc caccatacgg
     1801 ctatccgtca gtggcagcat cggactcacc ggatacggcg agcagcacga aatcgagtgg
     1861 accacctcgc tcacgcaagg cgcgaacctg ctgtccctac ccgtgttcgc gcgctcgcag
     1921 ggcgacggac tggtggtggc ggagatcgag catgaaggcc ggactcgccg gctgagcatg
     1981 gcgttgcacg tcgtggctcc cgaggatgac agcgcatgag tagcaagggc atcatgaggc
     2041 gtgcgcggcg cctgtatatc gcttcattgc tggcgctcat caccccggcg gtgttcgcgc
     2101 agtcgccgcc ggcgcgcaat cgtgacgatc tcgacgtcac gatgcatgtc atcgtggacc
     2161 cggatgccaa agtacccgac gaaatcgtgc gcaggattcc gctccccaag ccggcgcagc
     2221 cggcccctgg cgccaccagc acgagcacga ccgagtcgga caagcccaag ggacctgacg
     2281 gaaagggccg cgattttggt cagcaggttt ccgaggatgc gcgacagcgc tccgaggaag
     2341 cacgcaacaa caagaaaacc aaacctcctc cgcccggaca gccgcccggc aggccacctt
     2401 ctccacccgg tcgtccaccc acgccgggcg gttgaatttg tacggcgcgc ggcagtcgcg
     2461 ctgtgttttg cgttgatgct gttcagcggc ggcgcgcatg cggccgccgg cgacgccaag
     2521 gcacgcttcg ccgaagcgcg cacggcattc gaagccggag atttttcccg cgccttgttc
     2581 ctgtacgagc aatgtctggc gttgggaatg cagggacccg cagttcacta caacattggc
     2641 gtcgcggcgt atcgcgc
//
LOCUS       DASQJS010000417        16238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2469971, whole genome shotgun sequence.
ACCESSION   DASQJS010000417 DASQJS010000000
VERSION     DASQJS010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16238
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2469971"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..614
                     /locus_tag="VEW08_16375"
     CDS             120..614
                     /locus_tag="VEW08_16375"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ42370.1"
                     /translation="MPAISVATASDAGALASLRTAVARDMARQFGEGEWSASPSDADV
                     LQQLSASRVLVARSDTDIIGTVRLARARPWAIDASMFTPVATALYVLGLAVSPDARGR
                     GVGRSLMDAAKEAARSEPAGALWLDAYEHAAGAGPFYLKCGFRQVGRTSYREMPLLFY
                     EWLS"
     gene            complement(648..1469)
                     /locus_tag="VEW08_16380"
     CDS             complement(648..1469)
                     /locus_tag="VEW08_16380"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ42371.1"
                     /translation="MPYVTAAGDTQLYYKEWGRGRTVVLLHGWPLNADSWDDHALALA
                     DAGYRVIAYDRRGFGRSSQPWNGYDYDTLTDDLRSVFKHCLVSEATLVGFSMGGGEVA
                     RYMTKYNGAGVVQAGLIASIVPYKLKTDDNPHGTELKALEETTAAIKQDRPKFFAEFF
                     RKFYGVDTAEHPVSDEVLRWSLSMTMQAGLKPTLACIESIFKTDFRPDLASFKVPTLI
                     IHGTSDQNVSIDASARPAAKGIRNSKLIEYEGSPHGLLATDKQRITDHLLAFLKA"
     gene            1463..3928
                     /locus_tag="VEW08_16385"
     CDS             1463..3928
                     /locus_tag="VEW08_16385"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HYJ42372.1"
                     /translation="MAWKLLESDAEYLPAARAVAWDVSDYRRGPLLTAVVLSASMITR
                     RQTLKGGIALGACAAGAIRVADAAVADGAVTPPLMLWYRQPAEQWTEALPIGNGRLGA
                     MIFGGIARERLQLNEDTLYAGGPYDPSNPDALAALPQVRALIAEGKYAEAQDLANEKM
                     MARPIRMPSYQTVGELILTFGPSAYAQDYRRDLDLTTACAHVAFQRDGVNFTRESFAS
                     AVDQVIVTQITADRPGALSFRASFETPMPGAVATDAGHLVLNGRNVAQQGIESALRFQ
                     ARVAVIPKGGELHLRDGEFIVTGADSAMVLVAIATSFRRFDDVSGDPESITRAQIKAA
                     AAKTFGLLQAAHLSDHQKLFRRVAFELPDTPAAQQPTDLRIRNSQTSDDPRLAALYFQ
                     YARYLLIACSRPGTQPANLQGLWNDKLLAPWGSKYTININTEMNYWPAEPANLAECVR
                     PLVAMVKDLSVTGAVTARVHYGARGWVTHHNTDLWRATGPIDGAKWGMWPTGGAWLCK
                     HLWDHYDYGRDRAYLAEVYPLMKGAAQFFLDTLIKDPSGQFLVTSPSISPENLHPHGA
                     AICAGPAMDNQILRDLFANCISAAEILKVDGEFRSACAATRAKLPPDRIGKAGQLQEW
                     MEDWDMEAPERDHRHVSHLYALFPSEQISVRRTPELAAAVRRSLELRGDLSTGWAIAW
                     RINLWARLRDAEHTHGVIKLLLDPSRTYPNMFDAHPPFQIDGNFGGANGMMEMLLQSV
                     NGEIELLPALPRAWSTGSVRGLRARGGFEVDIAWRDGELLSAELRGAPGGDARVRYRE
                     KLIKLRVANQRRVTLTAADLGAG"
     gene            complement(3894..4910)
                     /locus_tag="VEW08_16390"
     CDS             complement(3894..4910)
                     /locus_tag="VEW08_16390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYJ42373.1"
                     /translation="MSHSTEFDRVRAAVNLHPAESTAAVTFEAAAQTARQVAADCLPL
                     GIALVMHLYPLCALRCVPLPWWSVANLRRAMLLRTIDRHSLILANAGSERAAGAHAPV
                     TLTRARDGVRVDGTYEYMSLAHVADIVMFSAPLAGGPDTMFCAADLKGDSVRIGPSRF
                     GGSMQLSDTCSVTFDNHHVPTHRCTGIPTEGALNCMAQYQRSWFQLLLGEGYLARIER
                     LQQQWKLPRPVEQIASLNELARLREYASRLLDEAAAPSAVESLARVTAAMKLRISWHA
                     QSIAAALRGVDDISASELGYLRLQPTSDERILKGIEDRCRVGKVGSPLPTLLTRHPDP
                     QRSV"
     gene            complement(4894..6000)
                     /locus_tag="VEW08_16395"
     CDS             complement(4894..6000)
                     /locus_tag="VEW08_16395"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HYJ42374.1"
                     /translation="MKLRYKILNGVAALFAVGIATLAITLAWESPCPTTPPAAVGAET
                     MRAVMQRCYGQPSVLTVERIAKPTAREGQILVKVRVASVNPFEWHMTTGKPYLLRLFK
                     GVGAPDSPRTGSDFAGVVESVGPGVTRFKPGDEIFGGSGGALAEYVLAREDGDVAIKP
                     AQLSFEQAAAIPIAAVTALQGLRDHGRISAGQKVLINGASGGVGTYAVQLAKYYGAEV
                     TGVCSTRNVELVRSLGADRVIDYSQEDFTEGEELYDLIFDTAGNHDVSDLLAVLKPQG
                     TLVGIGGSKKDPWIGPLWNMAKRRIIASFVDQKVLGFIASVTTPDLEFLAGLARDGKM
                     LSVIDRRYSLEETSAALEYIGTRRARGKVVVTLD"
     gene            complement(6002..7114)
                     /locus_tag="VEW08_16400"
     CDS             complement(6002..7114)
                     /locus_tag="VEW08_16400"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HYJ42375.1"
                     /translation="MKLRNKILIGVAGFFFTAFAALAITLSYEAPCPAVIPLADGTES
                     MRAVVQRCYGSPTNLRVERIAKPTPKDEQVLVKVHAASVNPAEWHRVTGKPYILRLDA
                     GIGAPKGFRVGFDYAGVVESVGAKVTRFKPGDEVWGVAGGALAEYVVVREAASILLKP
                     PQMSLEEASAIPIAAITALQGLRDHGHIAAGQKVLINGASGGVGTYAVQIAKALGAEV
                     TGVCSTRNVELVRSLGADHVIDYTKEDVTGTTERYDLILDNVGNHRLRALTAITKPTG
                     TLVVVSGPKTNAFLGPISRVIKLKLLEHFVDQRLVFFIANVNPKDLGFLADLVREGKM
                     KTVVDRRYPLAEAGVALEYIGTGRARGKVVVTVAEH"
     gene            7300..8352
                     /locus_tag="VEW08_16405"
     CDS             7300..8352
                     /locus_tag="VEW08_16405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYJ42376.1"
                     /translation="MSSTAGTAPAAMQRIFAWPRLRFTWAVSAAFGVMIGLGNGSRTA
                     LPADVIRAMLLGTVVLLVFGWLERWPRKLPRWLPRTMLQLFGIVFVVPIAVGLVCGLS
                     GVKDTHTYGGMLAAGLLFAPWIAIGAVLRQRDALARDQAFAFELERSEFERRESDARL
                     RLLQAQVEPHFLFNTLANVQALVDSGSPQASKVLTSLIAYLRAAVPRMHSQTTTLGTE
                     VELARAYLDLMQMRIPDRLQYVIHLDPAATRLSCPPMTLLTLVENAVRHGIDPSEAGG
                     RIDVDIWVRDQRCVVRVKDTGVGLKTVSRGLGTGLSNLRERLKLAFDGQAQLTLTEIE
                     PHGVCAEISFPARQAA"
     gene            8349..9128
                     /locus_tag="VEW08_16410"
     CDS             8349..9128
                     /locus_tag="VEW08_16410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HYJ42377.1"
                     /translation="MTRATALIADDEPLLREALVRLLGSAWPELDIVAQARNGREAID
                     LFDAHEPDICFLDVQMPGKTGVEAAQHIGRRAHLVFVTAFDQYAVQAFTEGALDYLVK
                     PIEPARLADTVRRLQDRLRAAQPALDPEALLQKLTMALNPNSASQSLRWVRASVGPTV
                     RLIAVQDIDFLRADEKYTQVAWRDEAGQTCEAVIRTALKDLIPQLDASQFAQVHRSVV
                     VNLRAVSHITRGANETADIHLKGRQETLPVSRTYLHLFKQM"
     gene            9138..9590
                     /locus_tag="VEW08_16415"
     CDS             9138..9590
                     /locus_tag="VEW08_16415"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYJ42378.1"
                     /translation="MLNRIIRPLLVAGFLAAMAVTAQAKPLTPAALESWLARYGAAWE
                     ARDAKLAGPLFTTDALYQEMPFDAPKKGRTGIEEYWRTVTADQRDVQFESKVVAVNGN
                     TGVAHWSAKFTLKSTGATIELDGVFVLEFDAKGQCTSLREWWHVRTGE"
     gene            complement(9645..11570)
                     /locus_tag="VEW08_16420"
     CDS             complement(9645..11570)
                     /locus_tag="VEW08_16420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYJ42379.1"
                     /translation="MPRHQSLTGNLLRAVLALAGFIGSAQAQVSCFVSADPAIYKLQL
                     KAASDANATLPLIDAELNATRGLPELDLNRMASLYAVRATTYQVLELDGDARAAAQEG
                     LQLVPDVNNPVHVELLSVFAENVYDHAGIDTAVKSVEAARSRLKPDSVAESCLRISLG
                     TLQYRQDRADLAIVNLMHAYQTGMLHERLVQRMIAASALSKVMRDMGDYTQALALNAE
                     VIEWNTQQNASLNLSVTRFLRGSILKEQRNFAAAIVEFVKARDLSVRLDDSQGIAFAD
                     LDLCQVQIEIGQLTEARQRCVNALRLFTIARSNDVVKHARAALAHIDLEEGHAARALV
                     ALNEILQNGAADMPPRDVAPLFKLRARANAAMGNFRESYDDIDESLRRTMAAEDARRL
                     QQSATLSARFETDRQLERNAELNRELATAQERQREQQRWTSIAIGTGAVVITLLTLQI
                     IAIRRHKRQLALLANQDSLTGLPNRRHTYELGSAAMARAASAHTPLTVAVIDLDHFKS
                     INDRCGHAAGDRVLQQFSIVCREALRDSDVFGRWGGEEFLLVMPGTTLDVALIALERL
                     RAQALKIELPSTGAGLRVGLSAGLASFEANVNTLDELIARADAALYRAKHEGRDLVRV
                     DEASFATASSGVRRQLR"
     gene            11724..13013
                     /locus_tag="VEW08_16425"
     CDS             11724..13013
                     /locus_tag="VEW08_16425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ42380.1"
                     /translation="MTSRVVPINDRITGAAMDQPLPKRQRNLIVLAACVLLPILVAAA
                     FWQLVPRGLRVPELELRIATVTRDLFRNDLVMRASAAPLHSVMLDAFQSGRVEEVLAT
                     DGALVAKGDLLFRLSNPQLRLELVAREADRAQQISNLSILRVAIESSETEHQRRLLDL
                     NFALTQARKQHARLAPLAKEGVVSAAVFEESEDRIAREAQALEDESTRFEVEARIKRD
                     GARQMERAIERLDAGLKVVNESIDGLAVRAPIAGRLTDFHLQLGEIVKPDQHIGRIDQ
                     PGEVKLVAEVDEFFLDSVATGTVGNASVNARDYPVVISRIFPQVDHGRFTIELLFAKD
                     APGGMSPGQSAETRLTLGESSPGLILPNDSFINDTGGAWVFVVARDGASAERRTIRLG
                     RRNNRQVEVAAGLEAGERVIVSNYGSFGKVERLQIAK"
     gene            13023..13682
                     /locus_tag="VEW08_16430"
     CDS             13023..13682
                     /locus_tag="VEW08_16430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ42381.1"
                     /translation="MLKFVNTTKKFIGGEVITTALNNINLEIQAGEYVAVMGPSGCGK
                     TTLLSIIGMLDTPDGGEYWFDGNNVAGYREDQLNELRRGRIGFIFQSFNLIEELSVEE
                     NVELALKYTSASAEERRERVAAMLDKLGVAHRAKHRPSQLSGGQQQRVAVARALVTRP
                     AVLLADEPTGNLDSAHGEEVMTLLQQINEEGTTIVMVTHSPAHAARAKRTVGLLDGAI
                     V"
     gene            13679..16099
                     /locus_tag="VEW08_16435"
     CDS             13679..16099
                     /locus_tag="VEW08_16435"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYJ42382.1"
                     /translation="MRRLDSHIVVAVLGLAVGLAAFLLLSGFARYSWSYNAQVPGAGH
                     VYLIKHRFNVEAGRPWWDKAPMMLREAALATPGVTDATGYLGWFPLNIQVDGQLHKLR
                     SLTVLPRFAEMMGLQAVKGDLVEALTQPDAFALTESAARKLFGTTDVLGRAFLLNSVE
                     TKGVARIAAILRDPPPNTTIPYESLNGLYLSLVPPMFRDEALHGQMGWPGHMLIRLHP
                     DASVAAVQDALQKFTDQAVRKLNLPPAAMAAIGEAKVMDIQLLPLREAYFDRNVVVDG
                     HTLGVDRGDRGVIAGLMAIGVLILVLAAFNYVNLATIRVIRRQREIGMRKVLGFGNGR
                     LAFQFLAESMLVSLLAAVIGLALAVLALPSVAGLVNRDLSSMLTPANAAAALGVGVIV
                     GFAVSIYPAWVAFRVRPAQVLAGRPDTESRSARRLRQALSVLQIAAAMGLASFTLAVA
                     WQTRFAINGSPGFDPAPLLVLEMNEGRKLEEDDTTRGFVAELGQQPAVAGVALAADAI
                     GRARHTWSQELTREGGRPVTIETREVTPPFFEEYGIRPVAGRLFDPRIDKYDPKFFPV
                     VLNELAAREVGFASAQQAVGRVLLQRSPLNSEMTPVHVIGIAPDIRLRSLREPPTAIV
                     YNLWSGGMTVTVRARESVADAAAAVRNLWPKYFPLTVLDMRPAKDIYAANYADDASLA
                     RLLTLATMVAMFIAAIGAYVLATDAVQRRTREIALRKLFGARRRDVGGLVAKEIGAVI
                     LQAAVVALPLAVLAIAQYLAPFSERTPVAYWALAVAGAAVVGVVGVAAARQARIAMRM
                     KPAVALRT"
BASE COUNT         2783 a         5129 c         5378 g         2948 t
ORIGIN      
        1 acccgggcat tggaggatca ctcccaatca gtcggcacgc tgcgtctcga tcccgatttc
       61 gatggcatgc gcgggcaatc ctgtgtcgcg gatgtgatcc gcaagctcga cgcgacgtga
      121 tgcccgcgat ctccgtggcg accgcgagcg acgcgggcgc gctcgcgtcg ctgcgcacgg
      181 ccgtcgcgcg cgacatggcg cggcagtttg gcgagggcga gtggtctgca tccccaagcg
      241 acgcggatgt gctccagcag ctgagtgcgt cgcgggtgtt ggtggcgcgc agcgataccg
      301 acatcatcgg caccgttcgc ctggcgcggg ccaggccatg ggccatcgac gccagcatgt
      361 tcaccccggt tgccacggcg ctctatgtgc tgggtctggc cgtgtcgccc gacgcgcgcg
      421 gacgaggtgt gggtcgcagc ctcatggacg cggcgaagga agccgcgcgc tcggaaccgg
      481 ccggcgcact gtggctggat gcttatgaac acgccgccgg agcggggcca ttttatctga
      541 agtgcggctt tcgtcaggtc ggacgtacgt cgtatcggga aatgccgctg ctcttctacg
      601 agtggcttag ctgagttatc ccggtttccc ggcccggcac cgatcaatca tgctttcaga
      661 aacgccagca ggtgatcggt gatgcgttgt ttgtcggtcg cgagcaagcc atgcggcgag
      721 ccttcgtact cgatgagctt cgagttcctg atgcctttcg ccgcgggcct cgcggaggca
      781 tcgatcgaca cattctggtc ggacgtgcca tgaatgatga gcgtcggcac tttgaatgat
      841 gcgaggtcgg gccggaagtc cgtcttgaag atcgactcga tgcaggccag tgtgggcttg
      901 agcccggcct gcatggtcat gctcagcgac catcgcagca cttcgtcact cacgggatgc
      961 tcggcggtgt cgacgccgta gaatttcctg aagaactccg caaagaattt cgggcggtcc
     1021 tgcttgatgg cggcggttgt ttcctccagc gctttcagtt cggtgccgtg cgggttgtca
     1081 tcggtcttga gcttgtacgg cacgattgac gcgatgagcc cggcctgcac cacgcccgcg
     1141 ccgttgtact tggtcatgta gcgcgcgact tcgccgccac ccatggagaa gccaacgagc
     1201 gtcgcttccg ataccaggca atgcttgaac acggagcgca gatcgtcggt cagcgtgtcg
     1261 tagtcgtagc cgttccaggg ttgtgaggac ctgccaaaac cgcgccggtc gtacgcgatc
     1321 acgcgatatc cggcgtccgc gagcgccagc gcgtggtcgt cccaggagtc ggcgttcagc
     1381 ggccatccgt gcagcagcac gacggtgcgg ccgcggcccc actccttgta atagagctga
     1441 gtgtcaccag cggcagtcac gtatggcatg gaagctcctc gagtcagacg cggagtattt
     1501 gcccgcggcg cgcgccgtgg catgggacgt ttctgactat cgccgtggtc cattgctgac
     1561 agccgtagta ctgtccgcct ccatgatcac gcgcaggcag acattgaaag gcggcatcgc
     1621 gttaggtgcc tgtgcggccg gcgcgattcg cgtggcagac gcggcggttg cggacggcgc
     1681 ggtcacgccg ccgctcatgc tctggtaccg ccagcccgcg gagcagtgga cggaagcact
     1741 gcctatcggc aacggccggc tgggcgcgat gatcttcggt ggcatcgcgc gcgagcgcct
     1801 gcagctcaac gaagacacgc tgtatgccgg tggaccgtac gacccatcga acccggacgc
     1861 actggctgca ttgccgcagg tccgcgcgct gatcgccgaa ggcaaatatg ccgaagcgca
     1921 ggatctcgcc aacgaaaaga tgatggcgcg gcccatccgc atgccgtcgt accagacggt
     1981 gggtgaattg atcctgacgt tcggcccgtc cgcctatgcg caggattacc ggcgcgacct
     2041 cgacctcact accgcgtgcg cgcacgtggc ctttcagcgt gatggcgtga acttcacgcg
     2101 tgaatcgttt gcgtcggcgg tggaccaggt catcgtcacg cagatcacgg cggaccggcc
     2161 aggcgccttg agttttcgcg cgagctttga aacgccgatg cccggcgcgg ttgcgaccga
     2221 tgcgggtcac ctggttctca acggccgcaa cgttgcccag cagggcatcg aatccgcgct
     2281 gcgattccag gcgcgggtgg cggtcattcc gaagggcgga gagctgcatt tgcgggacgg
     2341 tgagttcatc gtgaccggcg cggattcggc gatggtgctg gtggcgattg ccacgagctt
     2401 ccgccgcttc gatgacgtgt ccggcgatcc ggagagcatc acccgcgcgc agatcaaggc
     2461 ggccgcggcg aagaccttcg gattgctgca agcggcgcat ctgtcggacc accagaagct
     2521 gttccggcgc gtcgccttcg aactaccgga cactcctgcc gcgcagcagc ccaccgacct
     2581 gcgcattcgc aactcgcaaa ccagtgacga cccgcgcctc gcggcgctct attttcagta
     2641 cgcccgctac ctgctcatcg cctgctcacg gccgggcacg cagccagcca atctccaggg
     2701 cctctggaac gacaagctgc tcgcgccctg gggctcgaaa tacaccatca acatcaacac
     2761 ggagatgaac tactggcccg cggagccggc gaatctcgcc gaatgcgtgc ggccgctggt
     2821 ggcgatggtc aaagacttgt cggtcacggg cgcagtcacc gcgcgcgtgc actacggcgc
     2881 gcgcgggtgg gttacccatc acaacacgga cctgtggcgt gccacgggac cgatcgacgg
     2941 cgccaagtgg ggcatgtggc ccacgggcgg cgcgtggttg tgcaaacacc tgtgggatca
     3001 ctacgactac ggccgcgacc gcgcgtacct ggccgaggtc tatccactga tgaaaggcgc
     3061 ggcgcagttc ttcctggaca cgctgatcaa ggatccgtcg ggacagttcc tggtcaccag
     3121 cccgtcgatt tctcccgaga acctgcatcc gcacggcgcg gccatctgtg ccggaccggc
     3181 gatggacaac cagatcctgc gggacctctt tgccaactgc atttccgcgg cggagatact
     3241 caaggtggac ggagagtttc gctctgcctg tgcggcaacg cgcgcgaagc ttccgccgga
     3301 tcgcatcggc aaggcagggc agctccagga gtggatggag gactgggata tggaggcccc
     3361 ggagcgcgac catcggcacg tctctcatct ctatgcgctg tttccgagcg agcagatctc
     3421 cgtgcggcgc actcccgaat tggcggcggc ggtaaggcgc tcgctcgaat tgcgcgggga
     3481 tctgtccacg ggttgggcga tcgcctggcg catcaacctc tgggcgcggt tgcgggatgc
     3541 agagcacacc catggcgtga tcaaactgct tctcgatccg tcacgcacgt atcccaacat
     3601 gttcgacgcg cacccgccgt ttcagatcga cggcaacttt ggcggcgcca acggcatgat
     3661 ggagatgctg ctgcaaagtg tgaacggcga aatcgagtta ttgccggcgc tgcctcgcgc
     3721 ctggagcacg ggatcagtac gcggtctgcg ggcgcgcggg ggtttcgaag tggacatcgc
     3781 ctggcgcgat ggcgaactgc tctcggccga attgcgcggc gcacccggtg gcgatgcgcg
     3841 cgttcgctat cgcgagaagc tcatcaagtt gcgcgtcgcg aaccagcggc gagtcacact
     3901 gaccgctgcg gatctgggtg ccgggtgagg agagtgggga gtggactccc cactttcccc
     3961 acccggcacc tgtcctcgat gcccttcaag attctctcat ccgacgtcgg ctgcagccgc
     4021 agatagccga gttcgctcgc ggagatgtcg tcaacgccgc gcagcgcggc ggcgatcgac
     4081 tgcgcgtgcc acgagatgcg cagtttcatg gcggccgtga cgcgcgccaa tgattcgacg
     4141 gcgcttggtg cggcggcttc gtcgagcaag cgcgaagcat attcccgcaa acgcgccagt
     4201 tcgttgaggc tcgcgatctg ctccacaggc cgtggcagct tccactgctg ctgcaatcgc
     4261 tcgatgcgtg cgaggtatcc ctccccgagc aggagctgga accagctgcg ctggtactga
     4321 gccatgcagt tcagggcacc ttcagtcggt atcccggtgc atcggtgcgt cggcacgtga
     4381 tggttgtcga atgtcacgga gcaggtgtcg gacagctgca tacttccacc gaagcgggag
     4441 ggaccgatgc gtaccgagtc acccttcagg tccgctgcgc agaacatggt gtcaggaccg
     4501 ccggcgagcg gcgcgctgaa catcacgata tcggccacgt gcgcgagcga catgtattcg
     4561 taggtgccgt cgactcgaac gccatcgcgc gcccgcgtca gcgtcacggg cgcgtgcgcg
     4621 ccggcggccc gctcgctgcc ggcattcgcc aggatcagcg agtgacgatc gatggtgcgc
     4681 aggagcatgg cacgccgcag attggccacg ctccaccacg gcagcggcac gcatctcaag
     4741 gcgcacagcg ggtagagatg catgacgagc gcaatcccga gtggcagaca gtccgccgcc
     4801 acctggcgcg ctgtctgcgc ggcggcttcg aaagtgactg cggccgtgga ctcggcgggg
     4861 tgcaggttca ccgcagcgcg tacccgatcg aattcagtcg agtgtgacaa cgaccttccc
     4921 ccgcgctcgc cgagtgccga tgtattcaag agccgcgctc gtttcttcga gcgaatagcg
     4981 ccgatcgatc accgacagca tctttccgtc gcgtgcgagg cctgccagga actcgagatc
     5041 cggcgtggtg acactcgcaa tgaaccccag cactttctga tcgacaaacg acgcgatgat
     5101 tctgcgcttg gccatgttcc acaatggtcc gatccaggga tctttcttgg agccgccgat
     5161 gccgacaagc gtgccttgcg gcttcagcac ggccagcaag tcggagacgt cgtgattgcc
     5221 ggcggtatcg aagatgagat cgtagagttc ctcgccttcg gtgaagtctt cctggctgta
     5281 gtcgatgaca cgatccgcgc ccagcgaacg cacgagctcc acgtttcgcg tgctgcacac
     5341 gccggtgact tccgcgccgt aatacttcgc gagctgcacg gcatacgtac ccacgcctcc
     5401 cgatgcgcca ttgatgagca ccttttgtcc cgccgagatt cggccgtgat cgcgcaagcc
     5461 ctgcagcgcg gtgacagcgg cgatcggaat cgcggcagct tgctcgaacg acagctgcgc
     5521 gggtttgatg gccacgtcgc cgtcctcgcg tgccaggacg tactcggcga gcgcgccgcc
     5581 ggagccaccg aagatctcat cgcctggctt gaaacgcgtg acgcccgggc caacggattc
     5641 caccacgccg gcgaagtcgg aaccggtgcg cggagaatca ggcgccccga cgcctttgaa
     5701 gaggcggagc aggtagggct tgcctgtggt catgtgccat tcgaaaggat tgacgcttgc
     5761 gacgcgcacc ttgaccagga tctgtccttc ccttgcggtg ggtttggcaa tccgctcgac
     5821 ggtgaggacg ctcggttggc cgtaacaccg ttgcatgacg gcgcgcatgg tctccgcgcc
     5881 gacggcagct ggtggggtag ttgggcaggg agattcccag gccagcgtga tggccaacgt
     5941 ggcgatgccg acggcgaata gcgctgccac gccgttgagg atcttgtatc tgagcttcat
     6001 gtcagtgctc ggccaccgtg acgacgactt tgccccgcgc acgcccggtg ccgatgtact
     6061 cgagtgccac gccggcttcc gcaagcggat agcgccgatc gaccacggtt ttcatctttc
     6121 cttcgcgcac caggtcggcg agaaatccca ggtccttggg gttcacgttc gcaatgaaga
     6181 acacgagtcg ctggtcgacg aaatgttcga gcagcttgag cttgatgact ctcgagatcg
     6241 gcccgagaaa cgcattggtc ttgggcccgc tgaccacgac gagcgtgcca gtcggcttcg
     6301 tgatggcggt caaggcgcgc agccggtgat tgccgacgtt gtcgaggatg aggtcgtagc
     6361 gctccgtggt cccggtgaca tcctccttcg tgtagtcgat gacgtgatcc gcgccgagcg
     6421 agcgcacgag ctccacattg cgggtgctgc agacaccggt gacctcggcg cccagcgcct
     6481 tcgcgatctg caccgcgtag gttccgacgc caccggaggc gccgttgatc agcactttct
     6541 gcccggcggc aatgtgtccg tgatcgcgca ggccctgcag cgcggtgatg gcggcgattg
     6601 gaatcgcgct ggcttcctcg agcgacatct gcggcggctt caggaggatg ctcgcggcct
     6661 cgcgcaccac gacgtattcc gcgagcgcgc cgcccgcgac gccccacact tcgtcgccgg
     6721 gcttgaagcg cgtgaccttc gcgcccaccg attccacgac gcccgcataa tcgaatccga
     6781 cgcgaaagcc cttcggcgca ccgatgcctg cgtcgagtcg caggatgtag ggcttgccgg
     6841 tgacccggtg ccattcggcg ggattgacgc tcgcggcatg taccttgacc agcacctgtt
     6901 cgtccttggg cgtcggtttg gcaatccgct cgacccgcag attggtcggc gacccataac
     6961 aacgttgcac gacggcgcgc atcgattccg tgccgtcggc gagcggaatg acagccgggc
     7021 agggcgcctc gtaactcagc gtgatcgcca gcgcggcgaa cgcggtgaaa aaaaaccctg
     7081 ccacaccaat cagaatcttg tttctcagtt tcacgatgtt gttcctgtac ccctgacggt
     7141 cgaaactatg attcctcggc cgatcgcgtg ctggaccatg cgacggctga acgccagcgc
     7201 aggacgagtt acgtccggag cggacgaacg gcggtgccga cgggggtcgg actcgagccg
     7261 gccggcggtc ggatggccgc catgatttat agtcggtgca tgtcgtccac tgctggaacc
     7321 gctcccgccg ccatgcagcg catctttgcg tggccgcggc tgcgtttcac atgggcggtc
     7381 tcggcggcat ttggcgtgat gatcggcctc ggcaacggca gccggactgc cttacccgcc
     7441 gatgtgattc gcgccatgct tttgggaacc gtggtcctgc tcgtgttcgg ctggctcgag
     7501 cgatggccac gaaagttgcc caggtggctg ccacgcacga tgctccagtt gtttggaatc
     7561 gtgttcgtgg tgcccattgc cgtagggctc gtgtgcggac tatccggcgt gaaagacact
     7621 cacacctacg gcggcatgct ggccgcggga ttgctgttcg cgccgtggat tgccatcggc
     7681 gccgtcctgc ggcagcgcga tgcgctcgcg cgcgaccagg ccttcgcatt cgagctggaa
     7741 cgcagcgaat tcgagcgccg ggagtcggat gcgcgcctgc ggctgttgca ggcgcaggtc
     7801 gagccgcatt tcctgttcaa cacgctggcg aatgtgcagg cgctggtgga ctcgggctcc
     7861 ccgcaggcat cgaaggtatt gacgagtctc atcgcctatc tgcgcgccgc cgtaccgcgc
     7921 atgcactcgc agaccaccac cctcggaacc gaggtggagc tggcgcgcgc ctacctcgac
     7981 ctcatgcaga tgcgcatccc ggaccggttg cagtacgtga tccatctcga tccggccgcg
     8041 acacggctaa gctgtccacc gatgacgctg ttgacgctgg tggaaaacgc ggtacgccac
     8101 ggcatcgacc cgagcgaggc gggcggccgc atcgatgtcg acatctgggt tcgcgaccag
     8161 cgctgcgtgg tgcgggtgaa agacaccggg gtggggctca aaaccgtgag ccgcggtctc
     8221 ggtacggggc tatccaactt gcgcgagcga ttgaagctgg ccttcgatgg acaggcgcaa
     8281 ctcacgctga ccgagatcga gccgcacggt gtctgcgccg aaatcagttt tcccgcgcgg
     8341 caggcggcat gacgcgcgcc accgcactca tcgccgacga cgagccgctg ctgcgcgaag
     8401 cgctggtacg gttactcggc agcgcgtggc cggagctcga catcgtggcg caggcgcgca
     8461 atggacgcga agccatcgat ctgttcgacg cgcacgagcc cgacatctgt ttcctcgacg
     8521 tgcagatgcc gggcaagacg ggcgtggagg ctgcgcagca cattggtcga cgcgcgcacc
     8581 tggtattcgt cacggcgttt gaccaatacg cagtgcaggc cttcacggaa ggggcgctcg
     8641 actatctcgt caagccgatc gagccggcac gcctggccga caccgtgcgg cgattgcagg
     8701 atcgcctgcg cgccgcgcag ccggcactcg atccggaggc gctgctgcag aaattgacga
     8761 tggcgctcaa ccccaattcg gcgtcgcagt cgctgcgctg ggtgcgcgcc tcggtgggcc
     8821 ccacggtgcg gctgatcgcc gtacaggaca tcgatttcct gcgcgccgac gagaaataca
     8881 cccaggtggc atggcgggat gaggcgggcc agacttgcga ggcggtgatt cgcactgcgc
     8941 tgaaagatct catcccacag ctcgatgcct cacagttcgc ccaggtgcat cgctcggtgg
     9001 tggtgaatct gcgcgcggtc agtcatatca cgaggggagc gaacgaaacc gcggacattc
     9061 acctgaaggg aaggcaggaa acgttgcccg tgagccgtac ctacctgcat ctgttcaaac
     9121 aaatgtgagg tgctgagatg ctgaatcgaa tcattcgccc gctgctcgtt gcgggattcc
     9181 tggcggcgat ggccgtaacg gcgcaagcca agccgctcac gcctgcggcg ctcgaatcgt
     9241 ggcttgctcg ttacggcgcc gcgtgggagg cgcgcgatgc gaagctggcg ggtccgctgt
     9301 tcacgacgga tgcgctttac caggagatgc cgttcgacgc gcccaagaag gggcgcacgg
     9361 gcatcgagga atactggcgc accgtgactg ccgatcagcg cgatgtgcaa ttcgagtcga
     9421 aagtggtcgc cgtgaacggc aacaccggcg tcgcgcattg gtcagcaaag ttcacattga
     9481 agtcgaccgg cgccaccatc gagctggacg gcgtattcgt actcgagttc gacgcgaagg
     9541 ggcagtgcac gtcgttgcgc gagtggtggc acgtgcgcac cggtgagtga aggtgccggg
     9601 tggggaaagt ggggagtcca ctccccactt tccccacccg gcacctaccg cagttgccgg
     9661 cggacgccgc tcgaagcggt agcgaaactc gcctcgtcca cgcgcaccag gtcgcgtccc
     9721 tcgtgcttcg cccggtagag cgcggcatcg gcgcgcgcga tcagctcgtc gagcgtattg
     9781 acgttcgcct cgaatgacgc gaggccggcg ctcaggccca cgcgtagtcc agcacccgtc
     9841 gacggaagct cgattttcaa cgcctgcgcg cgcagccgtt cgagggcgat gagggcgaca
     9901 tccagcgtgg tgccgggcat cacgagcaga aactcttcgc cgccccatcg cccgaaaaca
     9961 tcggaatcgc ggagggcttc gcggcatacg atcgagaatt gctgcagcac cctgtcgccg
    10021 gcggcatgtc cgcaacgatc gttgatgctc ttgaagtggt cgaggtcgat gacggcgacc
    10081 gtgagaggcg tatgcgccga tgccgccctg gccatcgccg ctgatcccag ctcgtaggtg
    10141 tgacggcgat tgggcaggcc ggtcaggctg tcctgattcg ccagcaacgc gagctgccgc
    10201 ttgtgacgtc tgatcgcgat gatctgcagc gtcagcagag tgatgacgac cgcgcccgtg
    10261 ccgatggcaa tgctggtcca gcgttgctgt tcacgttgcc gctcctgcgc ggtcgcgagc
    10321 tcgcggttga gttcggcatt gcgctcgagc tgccggtccg tctcgaagcg agcgctcagc
    10381 gtcgcggact gctgcagccg ccgggcatcc tcggcggcca tcgtgcgacg cagtgactcg
    10441 tcgatgtcgt cataactctc gcgaaagttg cccatggctg cgttcgcgcg cgcgcgcagt
    10501 ttgaacaacg gtgcgacgtc gcgaggtggc atatcggcgg cgccgttttg caggatctca
    10561 ttgagcgcga cgagcgcgcg tgccgcatgc ccttcttcga ggtcgatgtg cgccagcgcc
    10621 gcgcgagcat gcttgaccac atcgttggac cgggcaatcg tgaacaggcg cagcgcgttc
    10681 acgcaacgtt ggcgggcttc ggtcaactgg ccgatttcta tctgcacctg gcaaaggtcc
    10741 aggtcggcga aggcgatgcc ctggctgtca tcgagcctca cgctcaggtc ccgtgccttc
    10801 acgaactcca cgatcgccgc cgcgaagttg cgctgctcct tgaggatcga gccgcgcagg
    10861 aatcgggtta ccgatagatt gagggaggcg ttttgctggg tattccactc gatgacttcg
    10921 gcgttcaacg ccagcgcctg cgtgtagtcg cccatgtcgc gcatcacctt ggacaatgcg
    10981 gacgccgcga tcatgcgctg cacgaggcgt tcgtggagca tgccggtctg ataggcgtgc
    11041 atcaggttga cgatggccag gtctgcgcgg tcctgtcgat actgcagtgt gccgagcgag
    11101 atgcgcaggc atgactccgc gaccgagtcc ggcttcagtc gcgaccgcgc ggcttcgacg
    11161 gacttgaccg ccgtatcgat gccggcgtgg tcataaacgt tttcggcaaa taccgagagg
    11221 agttcgacgt gaaccgggtt gttcacatcg ggaacgagct ggaggccttc ctgtgcggca
    11281 gcccgcgcgt cgccatcgag ttcgagcacc tggtaggtcg tcgcgcgaac cgcgtacagc
    11341 gacgccatcc ggttcagatc cagctccgga agtccccggg tggcattgag ttcggcatcg
    11401 atgaggggca gcgtcgcgtt agcgtcgctc gccgccttga gctgcaactt gtaaatggcc
    11461 ggatccgcgc tgacgaaaca cgacacctgc gcctgggccg agccaatgaa gcccgccagc
    11521 gcgagaactg cgcgtaagag attcccggta agtgattgat gcctgggcat ttggcgtgag
    11581 catagtgggg aagcgcccgg gtcgcccgtc aaagcttcac gaaactggcg gtgccggtac
    11641 caaccagttc acaacccgcc cgcaatttgc catgaaaaac tacaactgtc atagtatcaa
    11701 ccacacgagt attaggggag cgaatgacct ccagggttgt gccgatcaac gaccggatca
    11761 cgggcgccgc gatggaccag ccgctgccga agcggcagcg aaacctcatc gtgctggcgg
    11821 cgtgcgtgct gctgcccatc ctggtggcgg ccgccttctg gcagctggtg ccgcgcgggc
    11881 tgcgtgtacc ggagctggaa ttgcgcatcg ccacggtcac acgcgacctg ttccgcaacg
    11941 acctcgtcat gcgggcgtcg gcggcgccat tgcactccgt catgctcgat gcgttccagt
    12001 ccggccgcgt ggaagaggtg ctcgccactg acggcgcgct cgtcgccaag ggcgaccttc
    12061 tgttccgcct ctcgaatccg cagttgcggc tggagctggt ggcgcgcgaa gcggatcgcg
    12121 cgcaacagat ctcgaacctg tcgatcctgc gcgtggccat cgagtccagc gagaccgagc
    12181 accagcgtcg cctgctggat ctgaacttcg cgctcacgca ggcgcgcaag cagcacgcga
    12241 ggctcgcgcc gcttgccaaa gagggcgtgg tgtcggcggc ggtgttcgaa gagtccgagg
    12301 accgcatcgc gcgtgaagcc caggcgctcg aagacgagtc gacccgcttc gaagtggaag
    12361 cgcgcatcaa gcgcgacggc gccaggcaga tggaacgcgc catcgagcgg ctggatgcgg
    12421 ggctcaaggt ggtcaatgaa tccatcgatg ggctcgcggt gcgcgcgccg attgccggcc
    12481 gactcacgga cttccatctt cagctcggcg agatcgtgaa gcccgatcag cacatcgggc
    12541 gcatcgatca gccgggcgag gtgaagctgg tcgccgaggt cgatgagttc ttcctcgaca
    12601 gcgtcgccac cggcaccgtg ggcaacgcca gtgtcaacgc gcgcgactat cccgtggtca
    12661 tcagccgcat ctttccgcag gtggatcacg gccgcttcac catcgaacta ctgttcgcca
    12721 aggatgcgcc tggcggcatg agcccggggc agagcgccga aacgcgcctc acgctgggcg
    12781 agagcagccc gggcctgatc ctgcccaacg attccttcat caacgacacg ggcggcgcct
    12841 gggttttcgt cgtggcgcga gatggcgctt cggccgagcg ccggaccatc cgcctcggcc
    12901 gccgcaacaa ccgccaggtc gaagtcgccg cgggcctcga ggccggcgag cgcgtcatcg
    12961 tttccaacta cggctcgttc ggcaaggtcg aacgtctgca aatcgccaaa tagcgagggg
    13021 tcatgctcaa gttcgtcaat accaccaaga agttcatcgg cggcgaggtc atcacgacgg
    13081 cgctcaacaa catcaatctc gagatccagg ccggcgagta cgtcgcggtc atggggccct
    13141 cggggtgtgg caagacgacg ctgctgtcga tcatcggcat gctcgatacg ccggacggcg
    13201 gtgagtattg gttcgatgga aacaatgtgg ccggctaccg cgaggatcag ctcaacgagc
    13261 ttcggcgcgg gcgaatcggc ttcatcttcc agagcttcaa tctcatcgag gagctctccg
    13321 tcgaagagaa cgtggagctg gcgctgaaat acaccagcgc ctccgcggag gagcgccgcg
    13381 aacgagtggc ggccatgctc gacaagttag gcgtggcgca tcgcgcgaag catcgcccat
    13441 cgcagctctc gggtggccag caacaacgcg tggccgtagc gcgagcgctg gtcacgcgcc
    13501 cggcggttct gctggccgac gaacccacgg gcaacctcga ctccgcacac ggtgaagagg
    13561 tcatgacgtt attgcagcag atcaacgagg aggggacgac catcgtcatg gtcacgcatt
    13621 cgcccgcgca cgcggcgcgc gcgaaacgca ccgtggggct gctcgatgga gccatcgtat
    13681 gaggcgcctg gattcccaca tcgtggtcgc ggtgctgggg cttgcggtgg ggctcgccgc
    13741 atttctgctg ttgtcaggct tcgcacgcta ctcgtggagt tacaacgcgc aggtgccagg
    13801 cgccgggcat gtgtacctca tcaagcatcg cttcaatgtc gaggccggcc ggccctggtg
    13861 ggacaaggcg ccgatgatgc tgcgcgaagc ggcactggcg acacccggtg tcaccgacgc
    13921 cactggctac ctcgggtggt tcccgctcaa catccaggtg gacggccagc tgcacaagct
    13981 caggagtctg accgtgctgc ccaggttcgc cgagatgatg ggtctgcagg cggtcaaagg
    14041 cgacctggtt gaagcgctga cccagccgga cgcgttcgca ttgaccgagt ccgccgcgag
    14101 gaagctcttc ggcacgaccg atgtgctcgg acgcgcgttc cttttgaatt ccgtggagac
    14161 caagggcgtt gcgcgcatcg ccgcgatcct gcgtgacccg ccgccaaaca ccaccatccc
    14221 gtacgaaagc ctcaatggcc tgtacctgtc gctggtgcca cccatgtttc gcgacgaagc
    14281 gctgcacggc cagatgggtt ggcccgggca tatgctgatt cgactgcacc ccgatgcgtc
    14341 ggtggccgcg gtgcaggacg cattgcagaa gttcaccgac caggccgtgc gcaagctcaa
    14401 tctgccgccg gccgccatgg cggcgattgg cgaggccaag gtgatggata tccagctatt
    14461 gccattgcgc gaggcgtact ttgatcgcaa cgtcgtggtg gatgggcaca cgctgggtgt
    14521 ggatcgcggc gatcgcggag tgattgccgg gctcatggcg attggcgtgt tgatcctggt
    14581 gctggcggcg tttaattacg taaacctcgc gaccattcgt gtgattcgcc ggcaacgcga
    14641 aatcggcatg cgcaaggttc tcggtttcgg caatggccgg ctcgcgtttc agttcctcgc
    14701 cgaatcgatg ctcgtgtcgc tgttggccgc ggtcatcggt cttgcgcttg ccgtgctcgc
    14761 gctgccgtcc gtagccggac tcgtgaaccg cgatctctcc agcatgctca cgcccgccaa
    14821 tgccgccgcg gcgctgggcg ttggcgtgat cgtgggtttc gcggtctcaa tctatcctgc
    14881 ctgggttgcg ttccgcgtgc gtccagcgca agtgctcgcg ggccggccgg acacggagtc
    14941 tcggtccgcg cggcgcctgc gccaggcctt gtcggtattg cagattgccg ccgccatggg
    15001 gctcgccagt ttcacgctgg cggtggcatg gcaaacgcgc ttcgccatca acggttcgcc
    15061 tgggttcgat ccggcgccgc tgctcgtgct ggagatgaac gagggacgca agctcgagga
    15121 ggacgacacc acgcgtggct ttgtcgcaga actcggtcag caacccgcgg tggctggcgt
    15181 cgcgctggcg gccgatgcga tcggccgcgc gcggcatacc tggagccagg aactcactcg
    15241 agagggcgga cgccccgtca caatcgaaac gcgagaggtc acgccgccgt tcttcgagga
    15301 gtacggcatc cggcccgtcg ccggcaggct attcgatccc aggatcgaca agtacgatcc
    15361 caagttcttt cccgtcgtgc tcaatgaact ggcggcgcgc gaagtcggat ttgcgtccgc
    15421 acagcaggcc gtgggcagag tcctgctcca gagaagtccg ctcaattcag agatgacacc
    15481 ggtgcacgtc atcgggattg caccggacat ccgccttcgt tcgctgcgcg agccgccgac
    15541 cgcaatcgtc tacaacctgt ggtcgggtgg catgaccgtg actgtgcgtg cccgcgaatc
    15601 cgtcgccgac gccgcggcag cggtgcggaa cctgtggccg aagtattttc cgctgaccgt
    15661 gctcgacatg cgccccgcga aagacatcta cgccgccaac tacgccgacg acgcgagcct
    15721 ggccaggttg ctcacgctgg cgaccatggt ggccatgttc atcgcggcga tcggcgccta
    15781 cgtgctcgcc accgatgccg tgcagcgccg cacacgcgag atcgcgctgc gcaagttgtt
    15841 tggcgcgcgt cgccgcgacg ttggcgggct ggtggcgaaa gagataggcg ccgtgattct
    15901 gcaggcggct gtggtggcct tgccgctcgc ggtgctcgcc atcgcgcaat atctcgcgcc
    15961 tttctcggaa cgcacgcctg ttgcgtattg ggcgttggca gtggcggggg ccgcagtagt
    16021 gggagtggtg ggtgttgccg ctgcgcggca ggcgcgaatc gcgatgagaa tgaaaccagc
    16081 ggtcgcgctg cggacctgag gggtgtagcc ttttcctcga atccagggag aaggtcgtgc
    16141 gaatcaggtt ccggagagtc gtcttcacgc tggccgctgc ggcattggcc acgcgtgctt
    16201 ttgcacagga ggacccccac gcgaactgca cggcggtc
//
LOCUS       DASQJS010000418        21282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3724566, whole genome shotgun sequence.
ACCESSION   DASQJS010000418 DASQJS010000000
VERSION     DASQJS010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21282
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3724566"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..139
                     /locus_tag="VEW08_16440"
     tRNA            66..139
                     /locus_tag="VEW08_16440"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:98..100,aa:Cys,seq:gca)
     gene            152..238
                     /locus_tag="VEW08_16445"
     tRNA            152..238
                     /locus_tag="VEW08_16445"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:186..188,aa:Leu,seq:taa)
     gene            255..872
                     /gene="ccmA"
                     /locus_tag="VEW08_16450"
     CDS             255..872
                     /gene="ccmA"
                     /locus_tag="VEW08_16450"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HYJ42383.1"
                     /translation="MLTVQGVHLWRGDRHVLRGLSFEGQPGKCVLLTGKNGAGKTTLI
                     RAIAGLLDPEEGQVFWRGVPARTQRDEFHSELAYLGHEPPLKGDLSARENLKYSIGIR
                     RSVTTAEIDTALTRTGAISFADRPTRMLSAGQKRRVALAGVLLANAVLWLLDEPTTNL
                     DADGQQLVRDLISEQLARDGIVVAAVHHSLALPTEQIVALELGPS"
     gene            869..1564
                     /gene="ccmB"
                     /locus_tag="VEW08_16455"
     CDS             869..1564
                     /gene="ccmB"
                     /locus_tag="VEW08_16455"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HYJ42384.1"
                     /translation="MKARVEKTTALVAARLVAVRDLRLAFRKPSQVIQPLMFFMIVAT
                     LFPLTLTPEMSRLRDAAPGVLWIGALLSSLLALEFLFRDDAVDGTLEQFALSGQSLTW
                     MLFAKTCTHWMLTGLPLALSAPIAAAALGVSMEAMPGVVASLALGSFAMSLVGAIGAG
                     LTLGARRGGVLLSLLTLPLAVPILIFGARATQLAISGGDIAPPMYLLGAIAVLGVTLA
                     PLAAAAAVRIGLE"
     gene            1570..2307
                     /gene="ccmC"
                     /locus_tag="VEW08_16460"
     CDS             1570..2307
                     /gene="ccmC"
                     /locus_tag="VEW08_16460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HYJ42385.1"
                     /translation="MATWTWFHRLASPPHVYAFARRWTPWFGGFAAVAMLIALYGGLV
                     LAPPDYQQKDAFRMVYVHAPTAWLSLMIYTTMAVAAAVGLIWRMKVAHAAAAACAPIG
                     AWFTVAALGTGMLWGKPMWGAYWVWDPRLTAELVLLFLYFGYMGLRAGIEDPVKADKA
                     SAVLAVVGVINVPIIKFSVEWWNSLHQTATVMQLGKPKMNGDMLWPLLLMLLGFALYF
                     VTVLLIRLRAELLRRERNASWVREVVA"
     gene            2304..2459
                     /gene="ccmD"
                     /locus_tag="VEW08_16465"
     CDS             2304..2459
                     /gene="ccmD"
                     /locus_tag="VEW08_16465"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HYJ42386.1"
                     /translation="MSEFLDMGGYGKYLWPAFVLGFGIVVLNLVLALNSLASAKQEAR
                     RRMEMSQ"
     gene            2456..2917
                     /gene="ccmE"
                     /locus_tag="VEW08_16470"
     CDS             2456..2917
                     /gene="ccmE"
                     /locus_tag="VEW08_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HYJ42387.1"
                     /translation="MTPRQKRMTLVAGILAGVSVAAWLGARAFQGNLMLYYDPTDLAA
                     KMPPAGERFRLGGMVEKGSLKATSGTLDIQFLVTDYKHTVPVKYSGIVPDLFREGQGV
                     VAHGHMQEGTFVADEILAKHDEKYMPPEVAESLKNNPPPPNIPAASGSGGT"
     gene            2917..4899
                     /locus_tag="VEW08_16475"
     CDS             2917..4899
                     /locus_tag="VEW08_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HYJ42388.1"
                     /translation="MIPELGHFALILALVLAGAQAFFGIFGAARGDARWMAVVRPAVA
                     GQFVLVCTAIGALVHAFVTFDFSVLYVASNSNSALPTFYRVAALWGAHEGSLLLWAWI
                     LAAWTLAVAIASRNLPASFASRVMGVLGFVSFGFLLFTVATSNPFERLLPAAADGRDL
                     NPVLQDPALAIHPPMLYMGYVGFSVAFAFACAAMLEGKLDQVWARWTRPWTTMAWAFL
                     SVGIALGSWWAYYELGWGGYWFWDPVENASFMPWLVGTALIHSLAATEKRGVFKSWTL
                     LLAILAFSLSLLGTFLVRSGVLTSVHSFAADPTRGLFILAFLIIVVGGALALYAWRAP
                     MLRSPAGFEGTAREAFLLYNNVFLVVAGGMVFVGTLAPLVADALGITPISVGSPYFDS
                     LFPMLMLPLGALVAIGIHSSWKRGQLGNHRGRVLLGLGAAVLVGLVLVYGVYGAGKPL
                     TPIGMVLGIWVILSSLFDPVDRLRRRLTLPRAIVGMTLAHIGLGVGIVAISTVESYSV
                     ERDAAIAPGETLAMGRYAYRFDSIKEIEGPNYSGVRAEVTVLRDGVPMAVLQPEQRHY
                     WVQRSTLTEAGIASRWNLDLFVALSNNVGGGRWSVRAQLRPLIDWVWLSAAFMALGGV
                     LAVTDRRYRVRAVASEPAGAPASEPKMAQGSAAEAT"
     gene            4896..5429
                     /locus_tag="VEW08_16480"
     CDS             4896..5429
                     /locus_tag="VEW08_16480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HYJ42389.1"
                     /translation="MSRFLIPAAIFLALAGVLYVGVVHSPNKSTMQSALLGKTVPDFS
                     LPVLDNPGMNLTNTQLAGRPWVLNVWGTWCAACRDEHPVLLDIAKQNVLPLVGLDWKD
                     EDDAALEWLKQLGNPYSVVVADRDGRTAINFGVYGAPETFFIDADGRVQYRHVGAMTA
                     EVWQREFLSRLPRGGLP"
     gene            5426..5881
                     /locus_tag="VEW08_16485"
     CDS             5426..5881
                     /locus_tag="VEW08_16485"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HYJ42390.1"
                     /translation="MSWRIRTAALAVLGSVLLGISLSSAAFDIESMPNPEMQKRYDAL
                     VHELRCMQCQNQSIADSPVGLAEDLRRDVREQIIAGKTDAEIRESMVARYGNVILFRP
                     PFKPSTAWVWIAPFLLLLVGVLVAVRIVRQRASMVAGDESVVETDEPAR"
     gene            5878..6888
                     /locus_tag="VEW08_16490"
     CDS             5878..6888
                     /locus_tag="VEW08_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42391.1"
                     /translation="MILTFVLIAGTLASGAAILLMLPLMRPRIDARPAAGIAAAGMLM
                     VLLLGGAALYAAFSNYTWVEMPDAAETPAAMAAKLAKRLATEPNDLEGWMLLGRTYGT
                     LEQFPLAVRAYQRADRLANGKNADAIVGVAESLLAQDFEQIRGAAGHLFDRALVVEPD
                     NPKALLYGAFAALSRGEQSGARERFQRLLTLNPPPQIRDIINKQLLALDGAAAAPAPE
                     LAQGQGPKVAVHVTLAPSLAGKVPAGAALFVAARDPKSPGPPFAVKRLPAAFPVDVEL
                     SGADAMLESRRITDGQRLEVIARVALGGTPTATSGDPFGQVSYHVGKDGKLNIVIDRL
                     AP"
     gene            6915..7808
                     /locus_tag="VEW08_16495"
     CDS             6915..7808
                     /locus_tag="VEW08_16495"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HYJ42392.1"
                     /translation="MQAKHSLEFARAPAVWSMYPRIMMSRKPSLVPEGGEVPRIESRL
                     AKVVIDRKHLAQYSEICGAAAGATLPIAYPHILAMPLHLAMLGAEAFPVKLFGLVHVQ
                     NRIAMREPLSADEAAEIRCWIEGHRETERGQEFDLHTEYVVGGQPRWDETCTFLARKR
                     LPAGAARTTTISRTPESSADGVAIRSSSFRAPAGLGRRYGFISGDVNPIHMSDLSARA
                     FGFPKAIAHGMWSLGRLISDFESAQFDGGCELNVNFKLPIYMPAWLMLQRWDIENGSA
                     FALRDAQGEKPHLTGTLKALR"
     gene            7819..9090
                     /locus_tag="VEW08_16500"
     CDS             7819..9090
                     /locus_tag="VEW08_16500"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HYJ42393.1"
                     /translation="MKDQSPTVPASREQRRALLMAAAGFFCLMCGYYTLRPMREALAL
                     EVGVRYNSILFGTVLVCSAALLPIYWWLVGRTPRGRLPWLVSTPFVVVFLALAVGLTA
                     YPGDRTVAFVYFVALTTANLYLISLFWSAMADVWRPDLAKRFYGYVAAGGSAGAIIGP
                     QLVFGLVHEIGPAPLIVLACAFILGSAMFASLARRSLRRAPDGRAVPDAAIPVGGRAI
                     DDLKRLVRSPYLLGIAGIIVAGQIIGGFMYNEQGKLVAENYHSLADRAALFARLEFYV
                     NVLSLVFQAVVVTWLTRRGNLLLSLSAMPVVIGGSFVVMALYPAATVFLATQVLRRAA
                     DYGLGKPTREMLFTVLNPESKFKSKSLIDTVLQRGSDAVAQGLYLLIAGITLAGIAWM
                     CAALCVVLMFVTYALGKGFDARQPAATAPPP"
     gene            9087..10502
                     /locus_tag="VEW08_16505"
     CDS             9087..10502
                     /locus_tag="VEW08_16505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HYJ42394.1"
                     /translation="MNAAFSTLPAQRIDAGGKPHVRLGEVFRLAAPLMVTNAVQALLN
                     LTDLWFIGRLSTDAVAALGAIYWIMTCAILLFGGVGLAVQTFVAQAYGSRRGAYASRA
                     LWNALWASLALVPLFVGLAFAGPALLHPFGLDPHVEALALEYWMPRMGGAFIGSMGWA
                     AMGFFNGISAVRFTMLVVVVTTIANAILNQVFIFELGMGMKGSAWATNVAQIMGVALA
                     MVLFLGGDVGRRYRARLMWRPRWSVILAQLRVGMPIGVMYGADVLGLALSQMMIAQTS
                     ATGAAATQIVMALTSMAYMPTIGIALAGTTLVGQSIGAGDREWANRLGNVIIAMCAAL
                     MAVVAILLLVVGPWVVPLFVSSGGPSAGDVVTLGLTLLWPAAAYQIFDGLYCGSSFCL
                     RGAGDTRVPAATALVLSWFLFVPLAHTLVFAPGRGWIDGLPQYGLGAKGGWLALMIYC
                     TLLGCSMFWRWRHGAWRGVKL"
     gene            10565..12643
                     /locus_tag="VEW08_16510"
     CDS             10565..12643
                     /locus_tag="VEW08_16510"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HYJ42395.1"
                     /translation="MTVVRYKVEKGIAWLAMNNPPLNALSHALRAGVVNALDRALADP
                     KVRVVVIIGNERAFSSGADIKEFAGNAFYAEPFLPAVVDIVEAANKPVVAAISGACMG
                     GGLELALGCHYRVALANARIAFPEVKLGLIPGAGGTQRFPRLVGLEGAVNMIVSGATV
                     PASTFEGTALFDVLSSESLESAARDFALKLMKDARPIRRVRDLPCKHPQAEAFLQFAK
                     TGITSVSRGLAAPVRALEAVGAAVVQNFDAGIAIEQKVFAELMISDESKALRHAFFAE
                     RAASKVAGLSDKTPQRKISTVAVIGAGTMGGGIAMNFANAGIPVTILEAKQEALDRGI
                     AGIRKQYEGAVAKGELKPDEAAKRAGLITPSLDYAAAAKADLVIEAVFEDLAVKKTVV
                     QALDKVMKRGAILATNTSTLDVDRIAAFTKRPGDVLGMHFFSPANIMKLLEVVRAEKT
                     RPDALATVLSLAKRIGKTAVVSGVCDGFIGNRMINQYSHQALLLLEEGATPQQVDRAI
                     EKFGFAMGPFRMSDLAGNDISWHIRKRHYEERPKQRKMRIADRVCELGRFGQKTGLGW
                     YRYEAGKRDAIPDPVVGKIIDEERKALKIVAREIPDTEIADRLLYALVNEGAHILQEG
                     IAQRSSDIDVVYLAGYGFPVQRGGPMFYASQVGLGSVVRRMKQFGWKPAKLIVQLAAA
                     GKSFDGGYRG"
     gene            12636..13817
                     /locus_tag="VEW08_16515"
     CDS             12636..13817
                     /locus_tag="VEW08_16515"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HYJ42396.1"
                     /translation="MVDAVIVSTARTALAKSWRGAFNLTHGATLGAHVVKHAMERAKL
                     DPAAVEDVLIGCANPEGATGFNIARQIALRAGCPVTTSGMTVNRYCSSGLQTIALAAQ
                     RIMAGEGEVYVAGGVESISCVQNEHLNTFMAKEAWLDKNKPAIYWTMLQTAETVASRY
                     QISREAQDRYGVQSQQRAAAAQAAGRFTDEIVPMEVIAGVADRDSGRLMTRKVTVAAD
                     EGIRPDTNYEAVSKIRSATPGGVIAAGNASQFSDGAAAAVVMSAARAASLGLAPLGIF
                     RGFAVAGCEPDEMGIGPVFAIPKLLERAGKTVADIDLWELNEAFAVQVLYCADRLGIP
                     NDRLNVNGGAIAVGHPYGMTGARLVGHALIEGKRRGARLVVVTMCIGGGMGAAGLFEI
                     C"
     gene            13811..14536
                     /locus_tag="VEW08_16520"
     CDS             13811..14536
                     /locus_tag="VEW08_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42397.1"
                     /translation="MLKNVIAIIIAVAAGAIGYSFWMRKDAEESPLNVMVTQMRTRAI
                     IEHERTVTVWYKACPEVMGVNPQVMVIWPAKLNYELDLADTKLSLSPDGVLHVTTPPI
                     RADDPSVPSEVGAYIAQTSFWTLDSEQAIVMREMQKATPLARHLTSYFLRNDAGLSDH
                     FKEELETYLRGIAGALGVVITKIEIEIEIPVEVVPIPARPELSLCEGSVALANGLGFA
                     RRQADGTTIGIYPKKAAVKALMP"
     gene            14604..15638
                     /locus_tag="VEW08_16525"
     CDS             14604..15638
                     /locus_tag="VEW08_16525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ42398.1"
                     /translation="MEAVDVRGRFVWHQLMTRDVPGAKEFYSGLMGWKAQAWPLDPAY
                     TVCHAGDVPVAGIMEIPADLPAEVPAHWLQYIGTRDVDGTADAAVRAGGSVVKAPSDM
                     AGAGRYAVLKDPQGALFAIIDPENARAESVGTPPLGTFSWHELATSDNEAAFAFYSGL
                     FGWDAMTRMDMGPTGIYLVFGMNGVQRGGMYIKPPDWPAPPNWMPYAHVPSVDASAAN
                     LESVGAQVLVAPMDVPGGSRISSFMDPAGAPFAIHSFPSAAKPAAKPKAKLAVRAKAA
                     KKQVKKQARKQAIAARKAVKKVARKATRKVTKVMKVVKKAAKKKSAALRKSKAKMSKA
                     RKTKVARRKK"
     gene            15635..16603
                     /locus_tag="VEW08_16530"
     CDS             15635..16603
                     /locus_tag="VEW08_16530"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ42399.1"
                     /translation="MTERREVIKAGLATLAGTQVISAWAAEKEKDKDKPKPPPAAPLM
                     TKPVPSTGERLAAVGVGTNNYSPTTPEERAARREVLAGLTAAGASVIDTAPAYRQSEE
                     VIGELLADIGNRKHAFIATKVTAPEGNLAKGIAMIEESKRRLKTNTLDLVQIHSLNGV
                     DVLFPHLFELKKKGQVRYVGITTSSSTQHEQMVDLINSQPLDFVQVDYSLGNRAAAEA
                     VLPAAMAKRVAVLINLPFGGRREGNLFSRVSGTDLPEWAAEIDAKSWSQVFLKYVVSH
                     PAVTVAIPGMTKVAHLEDNLDALRGRMPDASQRARLEKYWDKNFDS"
     gene            complement(16666..17430)
                     /locus_tag="VEW08_16535"
     CDS             complement(16666..17430)
                     /locus_tag="VEW08_16535"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HYJ42400.1"
                     /translation="MTLRRPLIGLLTVVLGTMCIPTSAIAASKGRVLVYSGHTGYVHA
                     SIPAAVEALKAIGTRSGYEVDVSEDPEVFTAGKLAAYKAIVLVSNSTDPKKPESEWFT
                     GSRLEALQGFLKAGKGVVGLHAAADSHYNTGWYGRMIGGYFERHPKGTPKATITVVDA
                     KHPATAKLPRTITRNDEWYYYKDLDPTLHVLITVDPATIGDGGEADVNPNALVWYHDF
                     GGGRVFYSGLGHAPENYSEPYIVALLTGALKYAVGK"
     gene            complement(17456..18436)
                     /locus_tag="VEW08_16540"
     CDS             complement(17456..18436)
                     /locus_tag="VEW08_16540"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HYJ42401.1"
                     /translation="MNATAAESPYARLESLDKRFDALIAPDTKVEKIADDLKWSEGPL
                     WDARTKSLLFSDIPNNVIMKWNADKGVSEFLKRSGYTGAAPFTGREPGSNGLTFDLEG
                     RLTLCQHGDRRISRREADGTMVPVAVSYNGKKLNSPNDLVFDNHGALYFTDPPFGLPG
                     TFTDPNKELAFNAVFRVAPDGKITTVATELEAPNGLGFSPDYKTLYVANARASLPIWK
                     AYAVLADGSLDKGRVFADSAALYKEGDGVPDGLKVDVQGNLFATGPGGVLIYAPDGTL
                     LGRILTGVPTSNVAFGEDGSTLFITANHRVLRMRTLTRGVPLPVSAITSK"
     gene            18844..20853
                     /locus_tag="VEW08_16545"
     CDS             18844..20853
                     /locus_tag="VEW08_16545"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYJ42402.1"
                     /translation="MASLLLSFALLQSPAALADDEHNPVAHEPRMRESTANLGVIVKL
                     RKDGAGAAIAKLGNGADRTAELARRTGLALGLRREISDFMLASTVELGDASAAQILER
                     LRADPAIEHVSVDRRRFAHATTPNDLLFGNQWYLQNTEVSAVNAIGAWDREIGSTGVV
                     IAVLDTGVLYDHPDLGRADRGGKLLPGHDFVTASHMTNDGDGRDADPSDPGDWVDSTD
                     KANTQFANCETSGSSWHGTRVAGMIGALTNNSSGVAGLDWNSFILPVRVLGKCGGVDS
                     DILAGMRWAAGLHVVGVPDNPTPARILNLSLGSSDACEASYRDVIGELAARKVLAVIS
                     AGNEGTVVSSPANCPGVAAVAAIRHAGSKVGFSSLGPEVTIAAPGGNCVNINGGPCLF
                     SLDTTSNSGTTVPATNNFTDQINSNLGTSFSAPIVSGIAALMLSRNANLSTEQLLARL
                     REGARAFPASVADEPTIQACHVPVNDQDFQLAQCLCTTATCGAGMVNAAGSVAAAERP
                     IAAVALPSAVAPGQNVSLNASASAASCGRTLATFAWTVVAPTINPPVITGASTSVGTV
                     IAPASGSFTVRVTVTDDQGRTDFAEVTVAPTNVASLSPASAGAAPCATPVTSGPTPGT
                     PVTPTPAPNPAPTTPRRGGGGGGSLGLVTLFLLGLLCLAGERRRS"
     gene            21011..>21282
                     /locus_tag="VEW08_16550"
     CDS             21011..>21282
                     /locus_tag="VEW08_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42403.1"
                     /translation="MSRVYDRLESATLLLARSGAIKERLTGAWRQCLCNIEPEDVPRE
                     LRLPFLELTSAMQRERPLRGEDAVRATIRKMSNEEAEGHAALIVRM"
BASE COUNT         3630 a         6704 c         6955 g         3993 t
ORIGIN      
        1 cggggtcgcg agttcgagtc tcgtttcccg ctccaaatcc gcaggtaccc tccgttcggg
       61 cccgtggcta ggtggcagaa tggttatgca gcggcctgca aagccgtcta tgccggttcg
      121 attccgaccc tagcctccaa ccatggccca tgccccggtg gcggaattgg tagacgcaac
      181 ggacttaaaa tccgtcggcc gcaaggccat gccggttcga ttccggcccg gggcaccact
      241 cacatgctga cgaaatgctg accgttcagg gcgttcacct gtggcgcggc gatcgacatg
      301 tgcttcgggg cctgagcttt gagggccagc cgggcaaatg tgtattgctc acgggcaaga
      361 acggcgccgg caaaactacg ctcatccgcg ccatcgccgg gctgctggac cccgaggaag
      421 ggcaggtctt ctggcggggt gtgccggccc gtacccagcg cgacgaattt cattcagaac
      481 tggcatacct cgggcatgag ccgccgctca aaggtgattt gtcggcgcgc gagaacctga
      541 agtattccat tggcatccgc cgctccgtca ccaccgccga aatcgatacc gcgctcacgc
      601 gtactggcgc gatctcgttc gcggatcggc ccacccgcat gttgtccgcg ggtcagaagc
      661 gccgcgtggc cctcgccggg gtgttgctcg ccaacgccgt gttgtggctt ctcgacgaac
      721 cgaccaccaa tcttgatgcc gacgggcaac agctggtgcg ggatctcatc agcgagcagc
      781 tggcccgaga cggcatcgtg gtggccgccg tacatcattc gctggcgctc cccacggagc
      841 agatcgtggc cctggagctc ggcccatcat gaaagcccgt gtggagaaaa ccacggccct
      901 ggtagccgcg cggctggtgg ctgtgcgtga tttgcgtctc gctttccgga aaccttcaca
      961 ggtcattcag ccactcatgt tcttcatgat cgtggcaacg ctgttcccgc tgacattaac
     1021 cccggaaatg tcgcggctac gagacgccgc gccgggtgtc ttgtggatcg gcgcgttgtt
     1081 atcctcgctg ctggcgctcg agttcctgtt tcgcgatgac gcggtagatg ggacgctcga
     1141 gcagttcgcg ttatcggggc agtcgctgac atggatgttg tttgcgaaga cctgcacgca
     1201 ctggatgctc acggggctcc ctttggcttt gtccgcaccc atcgccgccg cggcgctggg
     1261 agtgtcgatg gaggcgatgc ccggcgtggt ggcgtcactg gcgctcggca gctttgccat
     1321 gagcctggtc ggcgcgatcg gcgcggggct cacactcggg gcccggcgcg gtggtgtgct
     1381 gttgtcattg ttgacgctgc cactggcggt gcccatcctg atttttggcg cgcgtgccac
     1441 gcagctcgcg atcagcggcg gtgacatcgc gccgccgatg tatttgttag gcgcgatcgc
     1501 cgtgctcggc gtgacgctcg cgccgctcgc cgcggcagcc gcggttcgga ttggactgga
     1561 gtagggccct tggcaacctg gacctggttt catcgtctgg catcaccgcc gcatgtctat
     1621 gcgttcgcgc ggcgctggac gccgtggttc ggcggattcg ctgcagttgc gatgttgata
     1681 gcgctctacg gcggactggt gctggcgccc ccggactacc agcagaagga cgcgttccgc
     1741 atggtgtacg tgcacgcacc cactgcctgg ctgtcgctca tgatctacac caccatggcg
     1801 gtcgccgccg cggtgggcct catctggcgc atgaaggtgg cgcatgccgc cgccgccgcg
     1861 tgtgcgccca tcggcgcgtg gttcacggtg gccgcactcg gcactggcat gttgtggggc
     1921 aagcccatgt ggggcgcata ctgggtatgg gatccccggc tcacggccga actggtgctg
     1981 ctgttcctgt acttcgggta catggggttg cgcgccggca tcgaggaccc cgtcaaagcc
     2041 gacaaggcga gcgccgtgct cgccgtggtt ggggtaatca atgtgcctat tatcaagttc
     2101 tcggtggagt ggtggaattc actgcaccag acggccacgg tgatgcagct cggcaagccg
     2161 aagatgaatg gcgacatgtt gtggccgctg ctgctcatgt tgctggggtt cgcattgtat
     2221 ttcgtcaccg tactgctcat ccggctgcgt gccgaactgc tgcgacgcga acgcaacgcg
     2281 agctgggtgc gtgaggtggt ggcatgagcg aatttctcga catgggcggc tacggaaaat
     2341 atctctggcc ggcgttcgtg ctcggcttcg gtatagtcgt gctcaacctg gtgctcgcgc
     2401 tgaactcact ggccagcgcc aagcaggaag cgcgccggcg catggaaatg tcgcaatgac
     2461 ccctcggcaa aaacggatga ccctcgtcgc cggcatcctg gccggcgtca gcgtcgccgc
     2521 ctggcttggt gcgcgcgcat tccagggcaa cctgatgctc tactacgacc cgaccgatct
     2581 cgccgcgaaa atgccgccgg ctggagaacg atttcgcctg ggtggcatgg tggagaaggg
     2641 cagcctgaag gcaacctccg gcacgctcga catccagttc ctggtcacgg actacaagca
     2701 cacagtgccg gtgaaatatt cgggcatcgt gccggacttg tttcgcgagg ggcagggcgt
     2761 ggtcgcgcat ggccacatgc aggaggggac attcgtcgcc gatgaaatcc tcgcgaagca
     2821 cgacgaaaaa tacatgccgc cggaagtggc cgagtcgttg aagaacaatc cgccgcctcc
     2881 gaacattccc gctgccagcg gcagcggagg cacctgatga ttcccgagct cggccatttc
     2941 gcgctgatcc tcgccctggt gctcgcgggc gcgcaggcgt tcttcggaat tttcggcgcc
     3001 gcacgcggtg atgcgcgctg gatggcggta gtcaggccgg cggtggccgg gcagttcgtg
     3061 ctcgtgtgca cggccatcgg cgcgctggtg cacgccttcg tcactttcga tttctccgtg
     3121 ctgtatgtcg ccagcaattc caattcggcg ctgcccacgt tctaccgtgt ggccgcactg
     3181 tggggcgcac acgaaggttc gttgctcctg tgggcttgga ttctcgcggc gtggacgctg
     3241 gcggtggcca tcgccagccg caatctgccg gccagtttcg cgagccgcgt catgggcgtg
     3301 ctcggcttcg tcagcttcgg cttcctgttg ttcacggtcg cgacttcgaa tcccttcgag
     3361 cgtctcttgc ctgcggcggc cgacggccgg gatctgaatc ccgtgctcca ggatccggcg
     3421 ctcgccattc atccgccgat gctctacatg ggctacgtcg gcttctccgt cgcgttcgcg
     3481 ttcgcttgcg ccgccatgct cgagggcaag ctcgaccagg tatgggcgcg ctggacgcgg
     3541 ccctggacga cgatggcctg ggcatttctg tcggtgggca tcgcactcgg cagctggtgg
     3601 gcatactacg aactcggctg gggtggctac tggttctggg atcccgtgga aaacgcctcg
     3661 ttcatgccct ggctcgtggg cacggcactc atccattcgc tcgcggccac ggagaagcgc
     3721 ggcgtgttca agagctggac actgctgctg gcgatcctcg cgttcagtct gagcttgtta
     3781 ggcacgttcc tggtgcgttc gggcgtgctg acttcggtgc attcgttcgc ggccgatccc
     3841 acgcgcggcc tgttcattct cgcgttcctc atcatcgtgg ttggcggcgc gctcgcgctg
     3901 tacgcctggc gcgcgccgat gctgagatcg ccagcgggct tcgagggaac ggcgcgcgag
     3961 gccttcctgc tctataacaa cgtcttcctc gtggtggccg gcggcatggt gttcgtgggc
     4021 acgctggcac cgctggtcgc cgatgccctg gggatcacgc caatctcggt ggggtcgccg
     4081 tacttcgact cgctgttccc gatgctcatg cttccgcttg gggccctggt cgccattggt
     4141 attcactctt cctggaagcg cgggcagctt ggaaatcatc gcgggcgagt cctgctgggc
     4201 cttggcgccg ccgtgctcgt cggcctcgtt ctcgtctacg gcgtctacgg cgccggcaag
     4261 ccgctcaccc cgatcggcat ggtgctcggc atctgggtca tcctgtcctc cttgttcgac
     4321 ccggtcgatc gcctgcggcg ccgcctgaca ttgccccgcg ccatcgtcgg catgacgctg
     4381 gcgcacatcg gcctcggcgt cggcatcgtg gccatctcga ccgtggagtc gtactccgtc
     4441 gagcgcgatg cggcgatcgc gccgggcgaa acgctggcga tggggcgtta tgcctatcgt
     4501 ttcgacagca tcaaggaaat cgaaggacct aactacagcg gggttcgcgc cgaagtcacc
     4561 gtgcttcgcg atggggtgcc aatggcggtg ctgcagcccg agcagcgcca ctactgggtg
     4621 cagcgctcga ctctcaccga ggcaggcatc gccagccgct ggaacttgga tctgttcgtg
     4681 gcgttgagca ataacgttgg cggtgggcgt tggagcgtcc gggcacagtt gcggccgctc
     4741 atcgactggg tctggctgtc ggccgcgttc atggccctgg gtggcgtgct cgctgtaacg
     4801 gatcgacgct atcgggtccg cgcggtggcc tcggagccgg caggtgcgcc ggccagcgaa
     4861 ccaaaaatgg cgcaaggatc ggcggcggaa gcgacgtgag ccgattcctg attcccgccg
     4921 cgatttttct cgcgctcgcg ggcgtgctgt acgtgggcgt cgttcattcg ccgaacaaga
     4981 gcacgatgca gtcggcgttg ttgggcaaga ccgtgccgga tttcagtctt ccggtgctcg
     5041 acaatcccgg catgaacctg acgaacacgc agttggccgg gcgtccgtgg gtgctgaatg
     5101 tctggggcac ctggtgcgcg gcctgccgcg acgaacaccc ggtgctgctc gacatcgcga
     5161 agcagaacgt gctgccgctc gtgggtctgg actggaaaga tgaagacgac gcggcgctcg
     5221 agtggcttaa gcagctcggc aatccttatt cggtggtggt ggccgaccgt gacggccgca
     5281 cggccatcaa cttcggggtg tatggcgcgc cggagacttt cttcatcgac gctgacggcc
     5341 gtgttcaata ccggcacgtc ggcgccatga cggccgaagt ctggcagcgg gagttcctct
     5401 cgcgacttcc gcgaggaggt ttgccgtgag ttggcgcatt cgtacggctg ccctggcagt
     5461 gctcggttcg gtgttgctgg gtatttcgct ctcatcggct gcgttcgaca tcgaatccat
     5521 gccgaatccg gagatgcaga agcgctacga cgcgctggtt cacgaactgc gttgcatgca
     5581 gtgccagaac cagagcatcg ccgattctcc ggtgggcctg gcggaagacc ttcgccgtga
     5641 cgtgcgggag cagatcatcg ccgggaagac cgacgcggaa attcgcgagt cgatggtcgc
     5701 gcgttacggc aacgtcatcc tgttccgccc gccgttcaaa ccgtcgaccg cgtgggtgtg
     5761 gatcgcgcct ttcctgctgt tgctcgtcgg ggtgttggtc gccgtgcgca tcgtccgcca
     5821 gcgcgcgtcg atggtggcag gcgacgaaag cgtcgtcgaa acggatgagc ccgcgcgatg
     5881 atcctgacgt tcgtactgat cgcgggaacc ctggcatccg gcgcggccat attgctgatg
     5941 ctgccgctca tgcggccacg gatcgatgcg cggccggcag cgggcatcgc cgccgccggt
     6001 atgctcatgg tgctgttgtt aggcggcgcc gcgctgtatg cagctttcag caactacacc
     6061 tgggtcgaga tgccggacgc tgccgaaacg ccggcggcca tggccgcgaa actggcgaag
     6121 cgcctggcca ccgagccgaa cgatctggaa ggctggatgc tgctcggtcg cacctacggc
     6181 acgctcgaac aattcccgct cgccgtgcgc gcctaccagc gcgccgatcg tctcgccaat
     6241 ggcaagaacg ccgatgccat cgtcggcgtt gccgaatccc ttctggccca ggacttcgag
     6301 cagattcgcg gggcagcggg gcacctgttc gaccgcgcac tcgtggtcga gcccgacaat
     6361 cccaaggcgc tgctctacgg cgcatttgcg gcactgagcc gaggtgaaca gtccggcgcg
     6421 cgcgaacgct tccagcgcct gctcacgctg aatccgccac cgcagatccg cgacatcatc
     6481 aacaaacagc tgctcgcgct cgatggtgcg gccgccgcac cggcgccgga gctggcgcaa
     6541 gggcagggcc cgaaggttgc ggtgcacgta acgctggcgc cttcactcgc aggcaaggtg
     6601 cccgcaggcg cagcattgtt cgtggcggcg cgtgatccga agtcaccggg cccgccgttt
     6661 gccgtcaaac gacttcccgc ggcgttcccc gtcgacgtgg aattgtcggg cgcggacgcg
     6721 atgctcgaat cgcggcgcat caccgacgga cagcggctcg aagtgatcgc gcgtgtcgcc
     6781 cttgggggaa cgccgaccgc aaccagcggc gacccgttcg gacaagttag ctatcatgtc
     6841 ggcaaagacg gaaagctgaa catcgtcatc gatcggctcg cgccctaagc cgcagtcgaa
     6901 atatcggggt tcccatgcag gccaagcatt cgctcgagtt cgcgcgcgcg cccgcggtct
     6961 ggtcgatgta tcccaggatc atgatgtcgc gaaaaccttc gctggtgccc gaaggcggcg
     7021 aggtgccgcg catcgagtcg cgcctggcca aggtggtcat cgaccgcaaa catctcgcgc
     7081 aatattcgga gatctgcggc gcggccgccg gcgccacgct gcccatcgcc tatccgcaca
     7141 tcctcgcgat gccgctgcac ctggcgatgc tcggtgccga ggcgtttccc gtgaagttgt
     7201 ttggcctggt gcacgtgcag aaccgcatcg cgatgcgtga gccgctgtcg gcggacgagg
     7261 ccgcggagat ccgctgctgg atcgagggtc accgcgagac cgagcgcggg caggagttcg
     7321 atctgcacac cgaatacgtc gtgggcggcc aaccgcgctg ggacgagacc tgcacattcc
     7381 tcgcgcgcaa acgcctgccc gcgggcgccg caaggaccac gaccatcagc cgcacaccgg
     7441 aaagcagcgc ggatggcgtg gcgatcaggt cgagcagctt tcgcgcgccg gccgggctcg
     7501 gacgccggta tggcttcatc tccggcgacg tgaatcccat ccacatgagc gacctctcgg
     7561 cacgagcctt cggcttcccc aaggcgatag cgcatggcat gtggtcgctg gggcgcctga
     7621 tttccgattt cgagtctgcg cagttcgacg gaggctgcga gctcaacgtg aacttcaaac
     7681 tacccatcta catgccagcc tggctgatgc tgcagcgttg ggatatcgag aacggctccg
     7741 ccttcgcgct gcgtgatgct caaggcgaga agccgcatct cacgggaacg ctgaaagcgc
     7801 tgcgataagc gtgcccacgt gaaagatcag tcgccgacgg tacccgccag tcgggaacag
     7861 cggcgcgccc tgctgatggc ggcggcgggc ttcttctgcc tgatgtgcgg ctactacacg
     7921 ctgcggccga tgcgcgaagc gctcgcgctc gaagtgggcg tgcgatacaa ctcgatactg
     7981 ttcggcaccg tgctggtgtg ttcggcggcg ttgcttccga tctattggtg gctggtgggc
     8041 cgcacgccgc gtgggcgttt gccctggctg gtttcgacac cattcgtggt ggtattcctc
     8101 gcgctcgccg ttggcctcac cgcctacccg ggcgaccgca ctgtggcctt cgtgtacttc
     8161 gtcgcactga ccacggcgaa tctctatctc atttcgctct tctggagcgc gatggcggac
     8221 gtgtggcgtc ccgatctcgc caagcgcttc tacggctacg tggcggcggg cggcagcgcc
     8281 ggcgccatca ttggtccgca actcgtcttc gggctggtgc acgagatcgg cccggcaccg
     8341 ctgatcgtgc tcgcctgcgc gttcatcttg ggctcggcga tgttcgcctc gttggcgcgc
     8401 cgctcactgc gccgcgcgcc ggacggtcgc gcggtgccgg atgcggccat tccggtcggc
     8461 ggccgtgcga tcgacgacct caagcgcctg gtgcgttctc cctatctact gggcatcgcc
     8521 ggcatcatcg tcgccgggca gatcatcggc ggattcatgt acaacgagca gggcaagctg
     8581 gtggcggaga actaccattc gctggccgat cgcgcggcgc tgttcgcgcg cctggagttc
     8641 tacgtcaacg tgctgtcgct ggtgttccag gccgtcgtgg tcacgtggct gacacgccgt
     8701 ggcaacctgc tgctgagcct gtcggccatg ccggtcgtga tcggcggcag cttcgtggta
     8761 atggcgctgt atcccgcggc caccgtgttc ctggcgacgc aggtgctgcg gcgcgccgcg
     8821 gactacgggc ttggcaagcc aacgcgagaa atgctgttca ccgtgctgaa tccggaaagc
     8881 aagttcaaga gcaagagcct cattgacacc gtgctgcaaa ggggctccga cgcggtcgcg
     8941 cagggactct acctgctgat cgccggcatc acgctcgccg gcatcgcatg gatgtgcgcc
     9001 gcgctgtgcg tggtgctcat gttcgtcacg tacgcactgg gcaagggatt cgacgcccgt
     9061 cagcccgccg cgaccgctcc cccgccatga acgcggcctt ttcaacgctg ccggcgcaac
     9121 gcatcgacgc cgggggcaag ccgcacgtca gacttggcga ggtattcagg ctcgccgcgc
     9181 cgctcatggt caccaacgcg gtgcaggcgc tgctcaatct gacggacctg tggttcatcg
     9241 ggcggctgtc caccgacgcg gtggcggcgc tcggtgctat ctactggatc atgacctgcg
     9301 ccatcctgct gttcggcggc gttggactgg cagtgcagac cttcgttgca caggcctacg
     9361 gcagccggcg tggtgcgtat gcctcgcggg ccttgtggaa cgcgttatgg gcctcgctgg
     9421 cactggtgcc tttgttcgtg ggactcgcgt tcgcgggtcc tgcgctgctg catcccttcg
     9481 gcctcgatcc gcatgtcgaa gcgctggcgc tcgaatactg gatgccgcgc atgggcggcg
     9541 cgttcatcgg ctcgatgggc tgggcagcca tgggcttttt caacgggatt tccgccgtgc
     9601 gcttcaccat gctggtggtg gtggtcacga cgattgccaa cgcgattctc aaccaggtgt
     9661 tcatcttcga gctcgggatg ggcatgaagg ggtcggcctg ggcgacgaac gtcgcccaga
     9721 tcatgggcgt ggcgctggcc atggtgctgt tcctgggcgg cgatgtcggc cgccggtatc
     9781 gcgccaggct gatgtggcgt ccgcgctggt cggtcatcct ggcgcagctc agggtcggca
     9841 tgccgatcgg cgtcatgtac ggcgccgacg ttctcggcct tgcgctctcg cagatgatga
     9901 ttgcgcagac cagcgccacg ggcgcggccg ccacgcaaat cgtcatggcg ctcacttcga
     9961 tggcctacat gcccacgatt ggcatcgcgc tcgcaggcac cacgctggtc ggtcaatcca
    10021 tcggcgccgg agaccgcgag tgggcgaatc gcctcggcaa cgtgatcatc gccatgtgtg
    10081 cggcgctgat ggccgtcgtc gccatcctgc tgctggtggt cgggccgtgg gtagtgccgt
    10141 tgttcgtcag cagcgggggc ccgtcggcgg gcgacgtcgt tacgctcggc ctcacgctgc
    10201 tgtggccggc ggccgcgtac cagatcttcg atggccttta ctgcggttcc agtttctgcc
    10261 tgcgtggtgc gggagatacc cgggtgccgg cggcgaccgc gctggtcctc tcctggttcc
    10321 tgttcgtgcc gctggcgcac acgctggtat tcgcgcccgg cagaggctgg atagacggcc
    10381 tgccacagta tgggctcggt gccaagggcg ggtggctcgc cctgatgatc tactgcacgt
    10441 tgttaggctg cagcatgttc tggcgctgga ggcacggcgc ttggcggggc gtcaaactgt
    10501 agacggcccg attgcgggcg ttgcctgatc gacgggcgcg cgccggcgtg cttatgatcg
    10561 gggcatgacc gtcgtccgct acaaagtcga aaagggcatc gcgtggctcg cgatgaacaa
    10621 tccgccgctg aatgcactgt cgcatgcgct gcgcgctggt gtcgtgaatg cgctcgatcg
    10681 cgcgctcgca gatcccaagg tccgcgtcgt ggtcatcatc ggcaacgaaa gggcgttctc
    10741 gagcggcgcc gacatcaagg aattcgccgg caatgcgttc tacgcggagc cgtttctgcc
    10801 cgccgtggtg gatatcgtcg aagccgcgaa caagccggtg gtggccgcca tcagcggcgc
    10861 ctgcatgggc ggtggcctcg aactcgcgct gggatgccac tatcgggtgg cccttgccaa
    10921 cgccaggatc gcgtttccgg aggtcaagct cggcctcatt cccggagcgg gcggaaccca
    10981 gcgtttcccg cgactggtgg gcctcgaggg tgcggtcaac atgatcgttt cgggggccac
    11041 cgttccggct tcgacgttcg aaggcaccgc attgttcgac gtgttgtcga gcgaatcgct
    11101 cgaaagcgct gcacgcgact tcgcgctgaa gctcatgaag gacgccaggc cgatccggcg
    11161 tgtgcgcgat ctgccttgca aacaccccca ggcggaggcg tttctgcaat tcgcgaagac
    11221 ggggatcacc tcggtgtcgc gcggtctcgc cgccccggtg cgcgcactcg aagcagtcgg
    11281 tgccgccgtt gtgcagaact tcgacgcggg catcgcgatc gagcaaaaag tgtttgccga
    11341 gctcatgatc agcgacgaat cgaaggcgct gcgccatgcg ttttttgctg agcgcgcagc
    11401 atcaaaagtg gcgggactct ccgacaagac accgcaacgc aagatttcca cggtcgcggt
    11461 gatcggcgcc ggcaccatgg gcggcggcat cgccatgaat ttcgcgaatg ccggcattcc
    11521 ggtgaccatt ctcgaagcga aacaagaagc actggaccgc ggcatcgccg gaattcgcaa
    11581 gcagtacgaa ggcgcggtgg cgaagggaga actgaaaccc gatgaagctg caaaacgcgc
    11641 cggcctcatc acaccatctc tcgattatgc cgcggcggca aaggcggacc tcgtcatcga
    11701 agccgtgttc gaggacctcg ccgtcaagaa gactgtcgtc caggcactcg acaaagtaat
    11761 gaaacgtggc gcgattctcg ccaccaacac ctcgacgctc gatgtcgacc ggattgcggc
    11821 gttcacgaag cgtccgggcg acgtgctcgg catgcatttc ttcagccccg ccaacatcat
    11881 gaagctgctg gaagtagtgc gtgcggagaa aacccgaccc gatgcactgg cgacggtgct
    11941 ttcgctggcg aagcgcatcg gcaagacggc ggtggtctcg ggcgtctgcg acggcttcat
    12001 cggcaaccgc atgatcaacc agtactcgca ccaggctctc ttgctgctcg aagagggggc
    12061 tactccgcag caggtggacc gcgccatcga gaagtttggg tttgccatgg gtccgttccg
    12121 catgtcggac ctggccggca acgacatcag ctggcatatc cgcaagcgtc attatgaaga
    12181 gcgccccaag cagagaaaga tgcgcatcgc ggatcgggtc tgcgaactcg ggcgcttcgg
    12241 acagaaaaca ggcctcggct ggtatcgcta cgaagccggc aagcgcgatg cgattccaga
    12301 cccggtggtc ggcaagatca tcgatgaaga acgcaaggcg ttgaagatcg tggcgcgcga
    12361 gattcctgat accgagatcg ccgatcgcct gctgtatgcg ctggtcaacg aaggcgcgca
    12421 catcctgcag gaaggcatcg cacaacggtc gtccgacatc gacgtggtgt atctggccgg
    12481 ttacggtttt cccgtgcagc gcggggggcc gatgttctac gccagccagg tcggcctcgg
    12541 gtcggtcgtc cggcgcatga agcagttcgg ctggaagccg gcgaagctca tcgtgcagct
    12601 tgccgcggcc ggaaagtcat tcgacggagg ctaccgtggt tgatgccgtc atcgtttcca
    12661 cggcgcgcac cgcgctcgcc aagtcctggc gcggggcctt caatctcaca catggcgcga
    12721 cgctcggcgc gcacgtggtc aaacacgcca tggaacgtgc gaagctcgat ccggccgccg
    12781 tcgaagacgt gctcatcggc tgcgccaatc ccgaaggcgc caccggcttc aacatcgcgc
    12841 gccagatcgc gctgcgcgcc ggctgtcccg tgacgacctc gggcatgacg gtgaatcgtt
    12901 actgttcatc gggcctgcaa accatcgcgc tggccgcgca gcgaatcatg gcgggcgagg
    12961 gcgaggtgta cgttgcgggt ggcgtcgaat ccatttcgtg cgtgcagaac gaacatctga
    13021 acaccttcat ggcgaaggag gcctggctcg acaagaacaa gccggccatc tactggacca
    13081 tgctgcagac cgcggagacc gtggccagtc gctaccagat ttcccgtgaa gcccaggatc
    13141 ggtatggcgt gcagagccag cagcgcgccg cggcggccca ggcggcgggg cggttcaccg
    13201 acgaaatcgt tcctatggaa gtcatcgccg gtgttgcgga tcgcgacagc ggccgcctca
    13261 tgacgcgcaa ggtgacggtg gccgcagacg agggcattcg cccggacact aactacgagg
    13321 cggtgtcgaa gatacgctcc gcaactccgg gcggtgtgat cgccgcgggt aacgccagcc
    13381 agttttccga tggagccgca gccgccgtcg tcatgagcgc cgcgcgcgcc gcatcgctcg
    13441 ggctcgcgcc gctcggcatc ttccgcggct tcgccgtggc cggatgcgag ccggatgaaa
    13501 tgggcatcgg tccggtgttc gccatcccga agttgctgga gcgcgccgga aaaaccgtcg
    13561 ccgacatcga tctgtgggag ctcaacgagg ccttcgcggt gcaggtgctc tattgcgccg
    13621 atcggctcgg catcccgaac gatcgcctca acgtgaacgg cggcgccatc gccgtcggac
    13681 atccgtatgg catgaccgga gcgcgcctcg tcggccatgc gctgatcgaa ggcaaacgcc
    13741 gcggcgccag gctcgtcgtg gtcaccatgt gcatcggcgg cggcatgggt gccgcggggt
    13801 tgttcgagat atgttgaaaa acgtcatcgc gatcatcatc gccgttgcag ccggcgccat
    13861 cggctattca ttctggatgc gaaaggatgc ggaggaaagc ccgctcaacg tgatggtcac
    13921 gcagatgcgg actcgcgcca tcatcgagca cgagcgtacg gtgacggtct ggtacaaggc
    13981 gtgtcccgaa gtcatgggcg tcaacccgca ggtcatggtg atctggcccg ccaagctcaa
    14041 ctacgagctc gacctggcgg ataccaaact atcgctgagc cccgacgggg tcctgcacgt
    14101 gacgacgccg ccgatccgcg cagatgatcc ttccgtcccg tcggaagtcg gggcgtacat
    14161 agcgcagacc tcgttctgga cgctcgacag cgagcaggcg atcgtcatgc gcgagatgca
    14221 gaaggccacg ccgctggcgc ggcatctcac ctcctatttt cttcgtaacg acgcgggttt
    14281 gagcgatcac ttcaaggaag aactggagac ctatctgcga ggaatcgccg gcgctctggg
    14341 cgtggtgatc acgaagatcg agatcgagat cgagatcccg gtggaggtcg tccccatccc
    14401 ggcgcggccc gagctctcgc tgtgtgaagg ctcggtggca ctcgccaacg gtttgggatt
    14461 cgcccggcgc caggccgacg gcaccaccat cggcatctac ccgaagaagg cggccgtaaa
    14521 agcgctgatg ccgtaaaaca attccgcacg gctcggggct atgatcgggg cgcaactctc
    14581 gtcccactta cggagatccg tccatggaag ctgtcgatgt gcgtggccga tttgtctggc
    14641 accagctgat gacgcgcgac gtgccgggcg ccaaagaatt ctattcagga ctcatgggat
    14701 ggaaagcgca ggcctggccg ctcgatcccg cctataccgt gtgccatgca ggggatgtgc
    14761 cggtagccgg catcatggaa atccccgccg atctacccgc ggaagtgccg gcgcactggc
    14821 tgcagtacat cggcacgcgt gacgtcgacg gcaccgcgga cgcggcagtt cgcgccggcg
    14881 gctccgtcgt caaggcgccc agcgacatgg cgggcgcggg gcgttatgcc gtgctcaagg
    14941 atccgcaggg tgcgttgttc gccatcatcg atccggagaa tgcgcgggcc gaatccgtgg
    15001 gcactccgcc gctcggtact ttttcgtggc acgaactggc gaccagcgac aacgaagccg
    15061 cctttgcgtt ctacagcggg ttgttcggct gggatgccat gacgcgcatg gacatggggc
    15121 cgaccggcat ttacctggtc ttcggcatga atggagttca gcgcggcggc atgtacatca
    15181 agccgccgga ctggccagcg cctccgaact ggatgccgta tgcccacgtg cccagtgtcg
    15241 atgcaagcgc cgccaatctc gaaagcgtag gcgcgcaggt cctggtcgcg ccaatggacg
    15301 tgcccggcgg cagccgtatc agctcgttca tggatccagc gggcgcaccg tttgcgattc
    15361 attcgttccc gtcagccgcg aagccagcgg cgaaaccaaa agcaaaactc gcggtcagag
    15421 cgaaagcggc gaagaagcag gtcaagaagc aggccaggaa acaggccata gccgctcgca
    15481 aggccgtgaa gaaggtcgcc aggaaggcga ctcggaaagt cacgaaggtc atgaaggtcg
    15541 tcaagaaggc cgcgaagaaa aagtccgcgg ccctcaggaa atcgaaagca aaaatgtcga
    15601 aagccaggaa aacgaaagtt gcacggagga aaaaatgacg gaacgtcgag aggtcatcaa
    15661 ggccgggttg gcaactctgg cgggaaccca ggtcatcagc gcctgggccg ctgaaaagga
    15721 aaaggacaag gacaaaccga aaccgccgcc cgcggcgcca ttgatgacca aaccagtgcc
    15781 ttcgaccggc gaacgcctgg ctgcggtggg tgtgggaacc aacaactaca gtcccactac
    15841 gcctgaagaa cgtgctgcgc gtcgagaagt gctggccggt ctcactgccg cgggggcttc
    15901 ggtcatcgac acggcaccgg cgtaccggca gtccgaagaa gtcatcggcg aattgctggc
    15961 cgacatcggc aatcgcaaac acgcgttcat cgccaccaag gtcaccgcgc cggaaggtaa
    16021 tctcgccaag ggcatcgcga tgatcgaaga gtcgaagcgc cgcctgaaga ccaacacgct
    16081 cgatctggtg cagatccaca gcctgaatgg cgtcgacgtt ctgttcccgc atctcttcga
    16141 gctcaagaag aaggggcagg ttcgttacgt cggcatcacc acgtcgagct ccacccagca
    16201 cgagcagatg gtcgacctca tcaattcaca gccgctcgat ttcgtgcagg tggactattc
    16261 gctcggcaac cgtgccgcag cggaggcggt tctgccggcc gcgatggcga agcgcgtggc
    16321 cgtgctcatc aacctgcctt tcggagggcg tcgcgagggc aatctcttct cgcgggtttc
    16381 gggcaccgac ctgcccgagt gggccgcgga gatcgacgcg aagagctgga gccaggtgtt
    16441 tctgaaatac gtggtctcgc atcccgcggt tacggtcgca attccgggca tgaccaaggt
    16501 tgcgcatctc gaggacaatc tcgacgcctt gcgcgggcgc atgcccgatg cctcccagcg
    16561 cgcacgtctc gagaaatact gggacaagaa cttcgattcg tgatcaccgg cagcaaaacg
    16621 cccgtgagcc ttgcaaggct cacgggcgtt cttgtttgaa gcggactatt taccgacggc
    16681 gtatttcagc gcgcccgtaa gcagggcgac gatgtacggc tcgctgtagt tctccggcgc
    16741 atgtccgagg ccgctgtaga acacccgtcc gccgccgaaa tcgtggtacc acaccagcgc
    16801 attgggattc acgtcggctt cgccaccgtc accgatggtc gcgggatcaa ccgtgatgag
    16861 aacgtgcagg gtcggatcga gatccttgta gtaataccac tcatcgttac gcgtgatggt
    16921 cctgggtaat ttggcggtgg ccggatgttt cgcgtcgacc accgtgatcg tcgccttcgg
    16981 tgtgcccttg gggtggcgct cgaaatagcc gccgatcatc ctgccgtacc agccggtgtt
    17041 gtagtgcgaa tcggcggcgg catgcagtcc caccacgccc ttgcccgcct tgaggaaacc
    17101 ttgcagcgct tcgagacgcg aaccggtgaa ccattccgac tcgggcttct tgggatccgt
    17161 ggaattgctc accagcacga tggccttgta agccgccagc ttcccggcgg tgaatacttc
    17221 cggatcttcg ctgacgtcga cttcgtaacc cgacctggtg ccaatcgcct tcagcgcttc
    17281 gacggcggcc ggaatcgatg cgtgcacgta gccggtatgc ccggagtaca ccagcacccg
    17341 gcccttgctg gcggcaatgg cgctcgtcgg aatgcacatc gttcccagga caacggtcag
    17401 gagaccgatc aacggtcggc gtagagtcat ggaatcccct tcgtgaaaaa acgatctatt
    17461 tgcttgtaat tgcgctgacc ggcaacggca cgccgcgcgt cagcgtgcgc attcgcagaa
    17521 cgcgatggtt ggcggtgatg aacagagtcg agccgtcctc gccgaatgcg acgttcgaag
    17581 tcggaacgcc cgtgaggatg cggccgagca gggtgccgtc cggcgcgtag atcagaacgc
    17641 caccgggacc ggtagcgaac aggttgcctt gcacatcgac cttgagacca tccggtacgc
    17701 catcgccttc cttgtacagc gcggcggaat cggcgaacac gcgacctttg tcgaggctgc
    17761 catcggcgag cactgcatag gccttccaga tgggcaagct ggcgcgcgcg ttggcgacgt
    17821 acagcgtctt gtaatccggc gagaacccaa ggccattcgg tgcttcgagt tcggtggcga
    17881 ccgtggtgat cttgccgtct ggcgcgacgc ggaacacggc gttgaatgcc agctccttgt
    17941 tgggatccgt gaacgtaccc ggcagcccga acggcggatc ggtgaaatac agcgcgccgt
    18001 ggttgtcgaa cacgaggtcg ttcgggctat tgagcttctt gccgttgtag ctcaccgcca
    18061 cgggcaccat ggtgccgtcg gcctcacgcc gcgagattcg ccggtcgcca tgctggcaga
    18121 gcgtgaggcg cccctcgaga tcgaatgtga ggccattgga gccgggctcg cggccagtga
    18181 atggcgctgc accggtgtag ccgctgcgct tcagaaactc cgagacgccc ttgtcggcgt
    18241 tccacttcat gatcacgttg ttcgggatat ccgaaaagag cagggacttg gtccgggcgt
    18301 cccacagcgg cccttcggac cacttcaggt cgtcggcgat tttttctacc ttggtgtccg
    18361 gcgcgatcag cgcatcgaag cgcttgtcta gtgattcgag tcgcgcgtat ggcgactccg
    18421 cggcagtggc gttcaaacag aaggtccccc cgaggaataa agcgagaaat cgcgaaaagt
    18481 ccattgaatt gatcccccca cgagatgtgc gccgaggatg cgggagccca aggatcgagg
    18541 caacacaacg gaccgagtga tcttcgggaa cctcggccgg cagtacacgc tggaagtccg
    18601 ctacagctgt ttgtgaggag tggacgggcg gcgccagtta cccgcgccgc ccggaccaag
    18661 caggttccag gaggccctat ataagaatat cattcaccat aatatacatt atgcgtagta
    18721 cgctatttat gcggggtgcg ttccgatcct gaattccggg gaacccttcc gaccctgaaa
    18781 caccacaaaa ttgcagaagc cacatcgtgg cgatcaggat ccgccatgca ccagcaatcg
    18841 ttgatggctt cgcttctgct ttcgtttgcg ctgctgcagt caccagccgc attggccgac
    18901 gacgaacaca atccggtggc ccacgagccc cgcatgcggg aatcgaccgc aaacctgggt
    18961 gtcatcgtca agctgcgcaa ggacggcgcc ggtgcggcca tcgcaaagct gggcaacggc
    19021 gctgatcgca cagccgaact cgcccggcgc acggggctcg cgctcggact caggcgcgaa
    19081 atttccgact tcatgctcgc cagcacagtc gagctcggcg acgcgagcgc cgcgcagatc
    19141 ctcgagcgcc tgcgggcgga cccggcgatt gagcacgtct cggtcgatcg acgccggttt
    19201 gcgcatgcga cgactccgaa cgatctgttg ttcggcaacc agtggtatct gcagaacacc
    19261 gaggtgtccg ccgtgaatgc cattggcgcc tgggaccgcg aaattggctc cacgggcgtg
    19321 gtcatcgccg tgctcgatac cggtgtgctt tacgaccatc ccgacctggg ccgcgccgac
    19381 cgcggcggga agctgttgcc gggacatgac ttcgtcaccg catcgcacat gaccaatgac
    19441 ggcgatggcc gcgacgcgga tccgtcggat ccgggcgact gggtcgattc caccgataag
    19501 gccaatacgc aatttgcgaa ttgcgagacc tcgggcagct cctggcatgg cacacgcgtg
    19561 gccgggatga ttggcgcact gactaacaat tcctcgggcg tcgccggcct ggactggaat
    19621 tccttcatcc tgccggtacg tgtgctcggc aaatgcggcg gcgtcgacag cgacatcctc
    19681 gctggcatgc gctgggcggc cgggctgcat gtggtgggcg ttccggacaa tcccacgccg
    19741 gcgcgcatcc tgaatctcag cctgggcagc agcgacgcgt gcgaagcgtc gtaccgcgac
    19801 gtcattggcg aactcgccgc gcgcaaggtt ttggccgtga tatcggctgg caacgaaggc
    19861 acggtggtgt catcaccggc aaactgcccg ggggttgcgg ccgttgccgc tattcgccat
    19921 gccggcagca aggtcggctt cagcagcctg ggacctgaag tgacgatcgc cgcgcccggc
    19981 ggcaactgcg tgaacatcaa tggcggcccc tgcctgttct cactagacac gacttccaac
    20041 agcggcacga cggtgcccgc gacgaacaac ttcactgacc agatcaacag caacctgggc
    20101 acgagttttt cggctccgat cgtctcggga attgcggcgc tgatgctgtc ccggaacgcg
    20161 aacctttcca ccgagcagct gctggcccga ctgcgtgaag gtgctcgcgc cttccctgct
    20221 tcggtcgccg acgagccgac catccaggcc tgccatgtgc cggtaaacga tcaggatttc
    20281 cagctcgccc agtgcctgtg caccaccgcg acatgcggcg ccggcatggt gaatgcagcc
    20341 gggtccgtcg cggctgccga gcggccaatc gcagcggttg cgctaccgtc ggctgttgca
    20401 cccggacaga atgtttcgtt gaacgcgtcg gccagcgccg cctcctgcgg aaggaccctc
    20461 gcgactttcg cctggacggt ggtggcgccc accatcaatc cacccgtcat caccggcgcg
    20521 agtacctcgg tcggcacggt catcgcgccg gcttctggat cgttcacggt tcgcgtcacg
    20581 gtgaccgatg accagggccg aaccgatttc gccgaagtta cagtcgcccc gaccaacgtg
    20641 gcttctctct cgccggccag cgccggtgcc gcgccctgcg cgactccggt gacttccggc
    20701 cccacgcccg ggacgcccgt gacgcccaca ccggcgccga atccggcccc aaccaccccc
    20761 cgtcgtggtg gtggcggagg aggctcactc ggcctggtca ccctcttctt gctaggtctg
    20821 ttgtgcctgg cgggagagcg tcgtcgctcc tagcgtattt cccgttgcaa ttgctaggtg
    20881 tcgcgctata ttcacgtcgt acgccattga cggatcaggg tctccgttac aactacaact
    20941 aaggcgttac gtgaggtctg cgacgcagag agggactcgc gccgctcgcc aagcgataac
    21001 gaaagaacca atgtcgcgtg tttacgatcg gctcgaatcc gccacgctcc tgctcgcccg
    21061 ttccggcgcc atcaaggagc gtttgaccgg tgcctggcgg cagtgtctct gcaacatcga
    21121 acccgaagat gtcccgcgcg agctccggct gccgttcctg gagctgactt ccgcgatgca
    21181 gcgtgagcgt ccgctgcgcg gcgaggatgc cgtgcgcgcc accatccgca agatgtccaa
    21241 cgaggaagcc gagggccacg cggcgttgat cgtgcgcatg tt
//
LOCUS       DASQJS010000419         6512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4667964, whole genome shotgun sequence.
ACCESSION   DASQJS010000419 DASQJS010000000
VERSION     DASQJS010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4667964"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1898)
                     /locus_tag="VEW08_16555"
     CDS             complement(<1..1898)
                     /locus_tag="VEW08_16555"
                     /inference="COORDINATES: protein motif:HMM:NF036483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42404.1"
                     /translation="MDKPVTSRKPARLAPAKEKPRASIVIHGARQNNLKNLSLEIPLH
                     ELVVVTGVSGSGKSSLVFDTLYAEGQRRYVETFSPYARQFLDRMDKPAVDRIDGIPPA
                     IAIDQTNPVRTSRSTVGTMTELNDHLKLLFARAGSLHCRNCGKPVRRDSAESIFTDAN
                     TRAAAAGDPRVYVCFPVTVPKNFKEKEVTALLESQGYARIHERNGDTLEVVQDRLRLG
                     GADRSRVMESLETALRVGRGRVNLHRVDDSPSSMEQGPKSIERWKYSSELHCADCDLS
                     YRDPVPSLFSFNSPVGACDSCRGFGRIIGIDYGLVVPDETKTLAGGAVKPWQTKSYKE
                     CQDDLVKFAKKRDIPLDTPWYALTDAQRQWVIEGEGKWEKKVWYGAKRFFEWLETKAY
                     KMHVRVLLSRYRAYTPCTTCNGSRLKPDALLWRIDGHNVHDLVLMSLDKSRAFFESLK
                     LPAPLDEAADLLLSEVRSRFAYLCEVGLGYLTLDRQSRTLSGGEVQRINLTTALGTSL
                     VNTLFVLDEPSIGLHPRDMQRVTQVMHRLRDAGNSLVVVEHDPQIMMAADRLLDLGPG
                     AGERGGEIVAFDTPDAVRANPKSLTGQYLSGAKVVAPRAPAPKPVSSEPRARMVPAQR
                     AIKLRGVT"
     gene            2062..3708
                     /locus_tag="VEW08_16560"
     CDS             2062..3708
                     /locus_tag="VEW08_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ42405.1"
                     /translation="MAEQKGIDAAHRRVIFASSLGTVFEWYDFYLYGSLVTIITRKFF
                     SGMNDTTAFLFALLAFAAGFFVRPFGALVFGRLGDLVGRKYTFLVTIVIMGASTALVG
                     VLPTYETAGVIAPIIFIILRLLQGLALGGQYGGAATYIAEHAPEGRRGLYTSWIQTTA
                     TLGFFLSLLVILACRSSMSPEQFDAWGWRLPFLLSIFLLVISAYIRLQLQESPVFQEI
                     KASGKLSKQPLTDSFGKWANLKIVILALLGATAGQAVVWYGGQFYALLFLEKTLLVDP
                     TSAQLSMAAALLIATPFFVVFGSLSDRIGRKRIVLAGCLLAALTYFPIFKGITHFANP
                     ALDHAVQTSPVTVIANPDGCSFQFDPVGKKQFKTGCDLAKGALARMGVSYTVGDDSLG
                     RDSDATVMVGEGSIEVKDLHGTLTAAQYKKAQDEVSAGIKELLVKAGYPAKADMQAFN
                     YPMVILLLAILVLYVTMVYGPIAAWLVELFPARIRYTSMSLPYHIGNGWFGGFLPVTS
                     FAIVAATGNIYNGLWYPVIVAAITIVIGGLFLRETKDSPA"
     gene            3846..6242
                     /locus_tag="VEW08_16565"
     CDS             3846..6242
                     /locus_tag="VEW08_16565"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ42406.1"
                     /translation="MKFRFSRGVAALFIAMPFASSAQEPATADSDLLEEIIVTAQKRA
                     TSLQDVPFSIAAMTAEDLEQSGATNIVEVARNVPGLYIADLGPGQSQVAIRGISAGQV
                     VRDQPGVKESVGIYLDESPISVALFTPDLDLYDLDRVEVLRGPQGTLFGAGSSSGTVR
                     YITAQPNIGEFGGSVDLTGNAPTDGEFGGSVKGSINVPIGENAAMRAVGYYTALPGFI
                     DSLYPDRATREDVNSGTRTGGRLAFRFEPSENFSITPRVVYQKLETDGYPRIDVYNIL
                     GNTYTTTEPVVNPGERGQVTQFREGITDDFMMADLKLDFGLGGDLGITAVTTYIDRQV
                     EVLRDASQLTGSVTIDLGGTADDARLNSPLYDDTDLQVLSQEVRLASTGEGAFQWLVG
                     AFYQQSDRKYGQTLPTPGYDALTQDLIGADSSDFNAPPDTPFYSRLSYDFQQFALFGE
                     ATYRFSPEWALTGGLRYYDYEEDRLLTFAGLFADQGYTDQPGSVSSDGVSPRVILAFS
                     PTEDVQFTAQIAQGFRLGGINDPLNVGLCQGTDLATYDGHPEFEDEITTNYEIGTKTR
                     FGGGRVTLNAAVFYTEIEDLQVIADAGSCSSRIVLNAQGESKGVEMELYLRPDENWDM
                     GLAMTFANTKITEDYLPGQPVGGVREGNRLPTAPDMQLSGTTAYTWNLTESLNSYVRF
                     TAQYVGSSFTQMADQEANFGLISNEAGRPAGSARLIDLGGIPANTNIEFDAELPSYAI
                     GNLRWGFGTDAWEASLFVNNLWDERAFLSIDRERGRSARVGYLTNPPRTYGVNFHMNF
                     "
     gene            complement(6314..>6512)
                     /locus_tag="VEW08_16570"
     CDS             complement(6314..>6512)
                     /locus_tag="VEW08_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42407.1"
                     /translation="NGDLLIDRHPTASNVLLVGAGSGHGFKHGPEVGRYAAELVTGKL
                     KKVEPRFSLETKAEQQNRAVH"
BASE COUNT         1137 a         2122 c         2045 g         1208 t
ORIGIN      
        1 tgcgtgacgc cgcgcagctt gatggcgcgc tgcgccggta ccatgcgcgc gcgtggctca
       61 ctggagaccg gcttcggcgc cggggctcgc ggcgccacga ccttcgcgcc cgacaggtac
      121 tggccggtaa gcgatttcgg attggcgcgc accgcatcgg gcgtgtcgaa ggcgacgatt
      181 tccccgccgc gttcgccggc gccgggcccc aggtccagca accggtcggc cgccatcatg
      241 atctgcggat cgtgctcgac caccaccagc gaattgccgg cgtcgcgcag ccggtgcatg
      301 acctgcgtca cgcgctgcat gtcgcgcgga tgcaggccga tggagggctc gtcgagcacg
      361 aacaacgtgt tgaccaacga ggtgccgagc gcggtggtga ggttgatgcg ctgaacctcg
      421 ccgcccgaga gcgtgcgcga ctgccgatcg agcgtgagat aacccagccc cacctcgcac
      481 aggtaggcaa agcgcgaacg cacttcggac agcaacaggt ccgccgcctc gtcgagcggc
      541 gcgggtagtt tgagcgactc gaagaatgct cggctcttgt cgagcgacat cagcacgagg
      601 tcgtgcacgt tgtggccatc gatgcgccac agcagcgcat cgggcttgag gcgcgagccg
      661 ttgcaggtgg tgcagggcgt gtacgcgcgg taacgcgaca acagcacacg cacgtgcatc
      721 ttgtaggcct tggtttcgag ccactcgaag aaacgcttgg cgccgtacca gaccttcttt
      781 tcccatttgc cctcgccctc gatgacccac tggcgctggg catcggtgag cgcgtaccag
      841 ggcgtatcga gcgggatgtc gcgcttcttc gcgaacttca cgagatcgtc ctggcactcc
      901 ttgtatgact tggtctgcca gggtttgacc gcgccgccgg ccagcgtctt ggtttcgtcg
      961 ggcacgacga ggccgtagtc gatgccgatg atgcggccga agccacggca ggaatcgcag
     1021 gcgcccaccg gcgagttgaa cgaaaacagc gagggcaccg gatcgcgata cgacaggtcg
     1081 cagtccgcgc agtgcagctc gctcgaatat ttccagcgct cgatggactt cggcccttgt
     1141 tccatggaag aggggctgtc gtccacacga tgcagattca cgcggccgcg ccccacacgc
     1201 agcgcggttt cgagcgactc catgacgcgc gagcggtcgg caccgccgag ccgcaggcgg
     1261 tcctgcacga cttcgagcgt gtcgccgttg cgctcgtgga tgcgcgcgta gccctgcgac
     1321 tcgagcaagg cggttacttc cttttccttg aagttcttgg gcaccgtcac cgggaagcac
     1381 acgtacacgc gtggatcgcc ggccgcggcc gcgcgcgtgt tggcgtcggt gaagatgctc
     1441 tccgcgctgt cgcgtcgcac gggcttgcca cagttgcggc agtgaagact tccggcgcgc
     1501 gcaaacagca gcttcaggtg gtcattgagc tcggtcatcg tgccgacggt ggagcgcgac
     1561 gtgcgcaccg gattcgtctg gtcgatggcg atggcgggcg gaatgccatc gatgcgatcc
     1621 accgccggct tgtccatgcg atcgaggaac tgccgcgcgt agggcgagaa ggtttcgacg
     1681 taacgccgct gaccttcggc atacagcgtg tcgaacacca gcgaagattt cccggaaccc
     1741 gagacgccgg tgaccaccac gagctcgtgc aacggaatct cgaggctcag attcttgaga
     1801 ttgttctggc gcgcgccgtg aatcacgatc gacgcgcgcg gcttttcctt cgcgggggcg
     1861 aggcgagcgg gtttgcgtga ggtgacgggc ttgtccatgt aacgaagaaa tggccgagac
     1921 gcggatcggt cattgtaccg accatgtcaa gcgggctgtg tggaaatgct ggtgacctgg
     1981 acgggtgcaa cgtcgggtcc ggggaaatgc ggcgattctc gcgggcttca agtatcgtaa
     2041 gcgccacagg aagggggatt cgtggcagaa caaaaaggca tcgacgccgc acaccggcgc
     2101 gtcatcttcg cgtcttcgct gggcaccgta ttcgagtggt acgacttcta cctctacggc
     2161 tcgctggtca ccatcatcac caggaaattc ttctcgggga tgaacgacac cacggcgttc
     2221 ctgttcgcgc tgctggcctt tgcggccggc ttcttcgtgc ggcccttcgg tgcgctggtg
     2281 ttcggccggc tcggcgacct cgtcggccgc aaatacacgt tcctcgtcac catcgtgatc
     2341 atgggcgcct cgaccgcgct ggtcggtgtg ctgccgacgt atgaaacggc gggcgtcatt
     2401 gcgccgatca tcttcatcat attgaggctt ttgcagggcc tggcgctcgg cggccagtac
     2461 ggtggggcgg cgacctacat cgccgaacac gccccggagg gcaggcgcgg cctctatacg
     2521 agctggatac agaccacggc cacgctgggc ttcttcctca gcctgctcgt catcctcgcg
     2581 tgccgctcct cgatgagccc cgagcagttc gacgcctggg gttggcgact gccgttcctg
     2641 ctctcgatct tcctgctcgt gatctccgcg tacatccgcc tgcaattgca ggagtcgccg
     2701 gtgttccagg agatcaaggc ctcgggcaag ctgtcgaagc agccgttaac cgattccttc
     2761 ggaaagtggg cgaacctcaa gatcgtgatc ctcgccttgt taggcgccac ggcgggccag
     2821 gcggtcgtgt ggtacggcgg ccagttctat gcattgctct tcctcgagaa aacattgctg
     2881 gtcgacccga cgagcgcgca gctctcgatg gccgccgcgt tgctgatcgc cacgccgttc
     2941 ttcgtggtgt tcggttcgct gtcggaccgg atcggccgca agagaatcgt gctcgcgggt
     3001 tgtttgctgg cggcgctcac gtatttcccg atcttcaagg gcatcacgca tttcgcgaac
     3061 ccggcgctgg accacgccgt ccagacttcg cccgtcaccg tgatcgcgaa tccggatggg
     3121 tgcagcttcc agttcgatcc ggtcggcaag aagcagttca agaccggctg cgatctcgcc
     3181 aagggcgctc tcgcgcgtat gggcgtttcg tacacggtcg gggacgattc cctcggccgc
     3241 gatagcgatg ccacggtgat ggtcggcgag ggctccatcg aagttaagga cctgcatggc
     3301 accttgaccg ccgcccaata caagaaggcg caggacgagg tctcggcggg aatcaaggaa
     3361 ttgctggtga aggcgggtta tcccgcgaag gccgacatgc aggccttcaa ctacccgatg
     3421 gtgatcctgc tgctggcgat cctcgtcctg tacgtgacga tggtctacgg ccccatcgcc
     3481 gcctggctgg tcgagctttt ccccgcgcgt attcgctaca catccatgtc gttgccgtat
     3541 cacatcggca acgggtggtt cggtgggttc ctgcccgtca cctcgttcgc catcgtcgcg
     3601 gccacgggca acatctacaa cggcctgtgg tacccggtga tcgtcgcggc catcaccatc
     3661 gtgattggcg gcctgttcct gcgcgaaacc aaggactccc ccgcgtaact cgttgcggcg
     3721 gcgccacgca tttgccccgg cgtggtgaat atcgacatac tccgcgccac aagcgcggca
     3781 gccgggggga cggtcgctca cgaccccgac gccgtgcaca aaaacgacag tcaaggagtg
     3841 gtcgcatgaa attcaggttc tcgcgcggcg ttgccgcgct gttcattgcc atgccatttg
     3901 catcttcggc gcaggagccc gcaacggctg attcggacct cctcgaggaa atcatcgtca
     3961 cggcgcaaaa gcgcgccacc tcgctgcagg atgtgccgtt ctccatcgcg gcgatgaccg
     4021 ccgaggacct cgagcagagc ggtgccacca acatcgtcga agtggctcgc aatgtgcccg
     4081 gcctctacat cgccgatctc ggccccggtc agagccaggt agccattcgc ggcatcagcg
     4141 ccggccaggt cgtgcgcgat cagcctggcg tgaaggaatc cgtcggcatc tatctcgacg
     4201 aatcacccat ctccgtggcg ctgttcacgc cggatctcga cctgtacgac ctcgaccgcg
     4261 tcgaagtgct gcgcggcccg cagggcacgc tgttcggcgc cggctcctcg tcgggtacgg
     4321 tgcgctacat caccgcgcaa ccgaacatcg gcgaattcgg cggttcggtc gacctgacgg
     4381 gcaacgctcc caccgacggt gagttcggcg gctccgtgaa aggcagcatc aacgtgccga
     4441 tcggcgagaa cgcggcaatg cgcgccgtgg gctactacac cgcgcttccc ggcttcatcg
     4501 actcgttgta tccggatcgc gcgacgcgcg aagacgtcaa cagcggtacg cgcaccggcg
     4561 gacgcctggc gttccgcttc gagccttcgg aaaacttctc gatcacgccg cgtgtcgtct
     4621 accagaagct ggagaccgac ggttatccgc gcatcgatgt ctacaacatc ctcggcaaca
     4681 cctacaccac cacggagccc gtggtaaatc ccggtgaacg tgggcaggtc acgcagttcc
     4741 gtgaaggcat caccgatgat ttcatgatgg ccgatctgaa gctcgacttc ggattgggcg
     4801 gcgatctggg catcactgcc gtcaccacgt acatcgaccg ccaggtcgaa gtgctgcgcg
     4861 atgccagcca gctcaccggc agcgtcacca tcgatctcgg cggcaccgcc gatgacgcgc
     4921 ggctcaattc gccgctgtac gacgacaccg atcttcaggt gctcagccag gaagtccggc
     4981 ttgcctccac gggcgaagga gccttccagt ggctggtcgg cgcgttctat cagcagtccg
     5041 atcgcaaata cgggcagaca ttgcccacgc cgggctacga cgcactgacg caggacctga
     5101 tcggcgccga cagctcggac ttcaatgcgc cgcccgacac gccgttctac tcacgcctga
     5161 gctacgactt ccagcagttc gcgctgttcg gcgaggctac ctaccgcttc agtcccgaat
     5221 gggcgctcac cggcggcctg cgctactacg actacgaaga agatcgcctg ctcacgttcg
     5281 cgggcttgtt cgcggaccag ggatataccg accagcctgg atcggtgagc tctgatggcg
     5341 tctcgccgcg cgtgatcctg gcgttcagcc cgaccgagga cgtgcagttc acggcgcaga
     5401 tagcccaggg cttccggctg ggcggcatca acgacccgct caacgtgggt ctgtgccagg
     5461 gcaccgacct cgcgacctac gacggtcacc cggaattcga agatgagatt acgaccaact
     5521 acgagatcgg caccaagacg cgcttcggcg gcggccgcgt gactctcaac gcagcggtgt
     5581 tctacaccga aatcgaggac ctgcaggtga tcgcggatgc cggcagctgc tcgtcgcgaa
     5641 tcgtgctcaa cgcgcagggc gagagcaagg gcgtggagat ggaactgtac ttgcggccgg
     5701 acgagaactg ggacatgggg ctcgcgatga cctttgccaa cacgaagatc accgaagact
     5761 atctacccgg tcagccggtc ggtggcgttc gcgaaggcaa tcgtctgccg acggctcccg
     5821 atatgcagct gtcgggcact accgcataca cgtggaacct gacggaatcg ctgaacagct
     5881 acgtgcgctt cacggcgcag tacgtcggat cttcattcac gcagatggcc gaccaggagg
     5941 ccaacttcgg cctgatctcc aatgaagccg gccgtcccgc cggctcggcg cgactgatcg
     6001 acctgggcgg cattccggcc aacaccaaca ttgaattcga cgccgagctg ccttcgtatg
     6061 ccatcggcaa cctgcgctgg ggcttcggta ccgacgcgtg ggaagcttcg ctgttcgtca
     6121 acaacctctg ggacgagcgc gccttcctgt ccatcgaccg cgagcgcggc cgcagcgcgc
     6181 gcgtgggcta tctcaccaac ccgccgcgca cctacggcgt gaatttccac atgaacttct
     6241 gaagttgcag acaggtgccg ggtggggaga gtggggagtt ggttactccc cgctttcctc
     6301 acccggcacc gttctaatga accgcccgat tctgctgctc cgccttcgtc tccaggctga
     6361 accgcggctc caccttcttc agcttccccg tcaccagctc cgccgcgtaa cgcccgacct
     6421 ccggcccatg cttgaaccca tggccgctcc ccgcgccgac taacaacacg ttgctcgccg
     6481 tcgggtggcg atcgatcagc aggtcgccgt tc
//
LOCUS       DASQJS010000420        11815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1215894, whole genome shotgun sequence.
ACCESSION   DASQJS010000420 DASQJS010000000
VERSION     DASQJS010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11815
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215894"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VEW08_16575"
     CDS             complement(<1..206)
                     /locus_tag="VEW08_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HYJ42408.1"
                     /translation="MKRALAVMALGLLASSAAVADGFRCGTKLVTEGASRSEVAAKCG
                     EPTDVTTARSVFRRPVVWGYGRPY"
     gene            322..972
                     /locus_tag="VEW08_16580"
     CDS             322..972
                     /locus_tag="VEW08_16580"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYJ42409.1"
                     /translation="MSFAAQWMAAVAGLGLAVAGGAARAADFDLTPAETQRVEKDEIV
                     IRATLDASARRGTVRAAMLIDAPPAVVFESMTRCADAVRYVPHLRLCRVRDRAADGSW
                     ALVEQEIDFGWYAPRLRYTFRADFVTDQSIAFRQVSGDFKANEGVWEFEPAEDGARTL
                     LRYRVYIDPPGYVPNWLARSTFKRELPQMLVDLRRFCEAEQALRAQANTSPEGLSP"
     gene            1018..2598
                     /gene="gshA"
                     /locus_tag="VEW08_16585"
     CDS             1018..2598
                     /gene="gshA"
                     /locus_tag="VEW08_16585"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HYJ42410.1"
                     /translation="MRAAIDRKFEQTLAALINSGEPGIIHGGRKGIERESLRVTPDGR
                     IAQTPHPRALGSALTNENITTDYSEALIELVSPTFRTSWELLQYLLDLHQFVYQHLGD
                     ELLWATSMPSIIDGDESIPIAQFGTSNVGRMKTVYRNGLGVRYGRVMQAISGVHFNYS
                     FPEQMWDSWADIVQSRERGQSFISDRYFHLLRNYRRHGWIVLYLFGVSPVVCNSFLRG
                     RNVTLPRLGKDTSYEPYATSLRMSDLGYRNRNQAGLSVSVNSLDEYVRDLSRAISTPH
                     EPYEQLGVKVGDEYRQLNANILQIENEYYSFIRPKRVARSGERPTKALRRAGVEYVEV
                     RALDVSAFDPVGVNQNKMRFLEMFLALCLMKDSQPIGVSEQDTLDANHVTVARRGREP
                     GLTLQRDGRGVSMSAWARELIESMQGIAEVFDRGDPAKPFSAALAMQAAKVQDVALTP
                     SARMLAELETTGESFFELGLRMSRLHKEYFLALYPPNVERQAEFRAAADESLAAQKAV
                     EAADRMSFEQYLSEYAAG"
     gene            2730..2930
                     /locus_tag="VEW08_16590"
     CDS             2730..2930
                     /locus_tag="VEW08_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42411.1"
                     /translation="MKTKNQTEQYLLELAVATAANEDGISPPTRDGVSATRNQAWSPL
                     EVWRTRVKTPAPTRTESAQIQG"
     gene            3001..4251
                     /locus_tag="VEW08_16595"
     CDS             3001..4251
                     /locus_tag="VEW08_16595"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYJ42412.1"
                     /translation="MAPTKNSTPSTSDASRLLTKGFRFLRFDEPLETEFRTEHRSRLQ
                     VWSRIAIIVSACTVIGFAILDHFVLSAEHSRITNMVRFGIHVPCVLIMLLLTSKRFYN
                     RWYELGISFVAPMFGIGTVIMSAFSPANEVPLVGGRLLLASFFFYFMLGLPLRAAARA
                     NLIVFAALVVAFLAGTMPTYTAAYLMFTLFTANVIGIAGSYALEHANRTAFLEHRQLT
                     EVATHDGLTTLLNRAAFEDQIRRVWQQAQRDRQTISVIMIDIDYFKAYNDRYGHVAGD
                     DCLRRVSSALRDAARRRPLDFVARYGGEELVAVLYGADKSYGESISRSLLTAVRELRI
                     PHANSETQPYVTVSVGVVAVDAYRLATHDAAVALADQALYAAKHQGRDRYVALEHSIQ
                     KVDPGATTVLPVRESASFREVSAG"
     gene            complement(4337..5245)
                     /locus_tag="VEW08_16600"
     CDS             complement(4337..5245)
                     /locus_tag="VEW08_16600"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HYJ42413.1"
                     /translation="MFAIAIHGGAGTLSGSETSAEHERAYSDGLSAALDAGYSVLERG
                     GTSLDGAIAAVCALEDDPLFNAGRGAVLTRDGLAELDASIMDGGTLGAGAVTGLRHVK
                     NPIALARLVMDRSPHVMLVGEGAEEFARLQGVEMVSNEYFRTQVRQQQLHRLLGGTAE
                     KENDLIAFGTVGAVALDASGNLAAATSTGGMTGKRWGRVGDSPIIGAGTYADNASCAV
                     SATGHGEYFIRAAVAHDICAQVEYLELPLEQAVANVLGKMKDLGGNGGVIAIDSRGEV
                     VLDFNSEGMFRGVRTSSGRNEVAIYR"
     gene            5374..8823
                     /locus_tag="VEW08_16605"
     CDS             5374..8823
                     /locus_tag="VEW08_16605"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF009554.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HYJ42414.1"
                     /translation="MLTRIRSLLVANRGEIAIRVMRAANELGVRTIAIYSQEDRFSLH
                     RTKADEAYLVGRGKAPVDAYLDIEDILRIAVEAKADAIHPGYGFLSENPDFVDACNRE
                     GVIFVGPTPEIMRTLGNKVAARNLAISAKVPVMPATPPLPADIEESKKQAAIVGYPVM
                     LKASWGGGGRGMRVIESDAQLTELLPVARREAKAGFGNDEVYLEKLVRKARHIEVQIL
                     GDTHGNLVHLFERDCTVQRRNQKVVERAPAAFLTDAQRNELCGYALQIGRAVNYRNAG
                     TVEFLQDAETGKFYFIEVNPRIQVEHTVTEAVTNIDLVKAQIRIADGAKIGEEASGVP
                     KQADIHLTSHALQCRVTTEDPENGFTPDYGKITAYRSPAGFGIRLDAGTAYAGAILTR
                     SYDSMLVKVTAWAPNPAEAIARMHRALWEFRIRGVSTNLRFLDQVITHPKFVNADYTT
                     KFIDETPELFKWEKKRDRATRILSFIGETIVNGNPEVKGRQKPKSMVLARLPKVNVAK
                     TPPPGTKQKLDELGAKGFAKWMLAQKQVLITDTTMRDAHQSLLATRMRQPDMAAIAPY
                     YAHMLPQLFSVECWGGATFDVAMRFLREDPWSRLEQLRTLMPNLLLQMLLRSSNAVGY
                     TNYPDNVVRAFVKQAAVSGVDVFRVFDSLNWVENMRVAIDAVLETGKLCEAAICYSGN
                     LSSPNETRYTLDYYLEMGRKLKDAGTHILGIKDMAGLCQPNAAYTLVKALKEELKLPV
                     HFHTHDTSGIAAASVLAAVAAGCDAVDGAIDAMSGFTSQPNLGSIIEALRYSERDPGI
                     DHASVRAISMYWEQVRKNYLAFESDMRAGASEVYVHAMPGGQYTNLKEQARSVGLDDS
                     RWPEVSQAYADVNQMFGNIVKVTPSSKVVGDMAILMVSSGLTKEQVVDPNFEVAFPES
                     VVQMMRGDLGRPEGGWPAGIQKKVLKDQKPLEQRPGALMPPLDLVAERRKLDEKLGRA
                     ATDTQFASYLMYPRVFLDYAKDRTAYGDCAILPTPVFFYGMEPGDEVSVDIERGKTLI
                     VRFVALSEVRDDGTRQVFFELNGQPRSIVVTDRSQVAKRPPQRKMEPGNARHVGAPMP
                     GTIATVKAIVGQKVAKGDLLLTMEAMKMETSVRAEADGTVAEVLVKPGVQVDAKDLLV
                     VLS"
     gene            complement(8870..9001)
                     /locus_tag="VEW08_16610"
     CDS             complement(8870..9001)
                     /locus_tag="VEW08_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42415.1"
                     /translation="MVDTGHQHVRYVFRTCWLGVDDLHRVRQCRTQWLDSLPGLARH"
     gene            complement(9024..10652)
                     /locus_tag="VEW08_16615"
     CDS             complement(9024..10652)
                     /locus_tag="VEW08_16615"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HYJ42416.1"
                     /translation="MKALSIVLAAALATAMAAQAAPPHLAKQGSTQQLIVDGKPFLIL
                     GGELGNSSSSSANYMRPHWPRLKAMNLNTVLAPVSWELIEPHEGKFDWTSVDELLRDA
                     RAHDLKLVILWFGAWKNSMSTYVPSWVKRDQNRFPRAQLANGSSVEILSAFSTNTRDA
                     DAQAFGAFMEYLKRVDGEKNTVLMVQVENEIGMLPIARERGAPAEKLFAGPVPPELIR
                     SLSKREKLEPELQALWSKHGARTSGTWAEVFGNEEAGAEVFTAWHYARFVEALVVAGK
                     KRYDIPLYVNVALNRTGRKPGEYPSGGPLPHLIDVWKAGTPTTDMLSPDIYFPNFAQL
                     AARFNRPDNVLFIPEANNATNSQVSANAFYSFGALDSLGFSPFSIESLGDAPNALSQS
                     YEVLKQLTPVLLDARGRGRLGGFRATINEDGSVIDSPVKKMLGGIEFTVTFIDPWTPR
                     EKQTIADHGGLVVHAGDEDFWFAGQGMTVTMRGVGDGPPLVGIDVAEEGEFDEKGKWT
                     PGRRLNGDQTHQGRHIRLPPGPFQIQKVRLYRYR"
     gene            complement(10649..11593)
                     /locus_tag="VEW08_16620"
     CDS             complement(10649..11593)
                     /locus_tag="VEW08_16620"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYJ42417.1"
                     /translation="MEAAQNFNQMLAMRVFVRVVEAGTFTNAANSLRMPKPTVTKLVQ
                     GLESHLRIKLLNRTTRRVTVTPDGSAYYERVVRLLGDLEDIESSVTHAKERPRGRLRI
                     DAGAAMSYYLILPALPGFLAKYPEIDIECGVSDRPADLIGDNVDCVIRGGPLLEPSLV
                     ARHIGDMRWLTCATPSYFARHPKPTHPTDLESGHVLAGYFFPRSGRMRPLIFERDGKR
                     IEIAPSPRVAVNDSGAHFASLRAGTGLGQLLSFMVSASNATQELVPVLQDWQPEPLPV
                     HVLYPANRHLSTKVRVFVDWAAELFSQSPCTRLLTSGA"
     gene            11726..11815
                     /locus_tag="VEW08_16625"
                     /pseudo
     CDS             11726..11815
                     /locus_tag="VEW08_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
BASE COUNT         2227 a         3814 c         3658 g         2116 t
ORIGIN      
        1 aagtacggcc gcccatagcc ccagaccacg ggccgccgga acacgctgcg cgcggtggtc
       61 acgtcggtgg gctcgccgca cttggcggcc acttcggatc gcgacgcgcc ttcggtcacg
      121 agcttggtgc cgcagcgaaa cccgtccgca actgcggccg atgaggccag caggccgaga
      181 gccattacgg caagtgcccg tttcatgatc caaacgatag gctcatcctg cttaacggac
      241 aatgaacaca tcggcgtttt gatccggatg aaccatttgg cccgcgagcc gcactcactt
      301 gaggatgggg tatcgttgac catgagcttt gcagcgcaat ggatggcggc tgtagcgggc
      361 ctcgggctgg ctgttgccgg tggcgccgcg cgggccgcgg atttcgatct caccccggcc
      421 gagacgcaac gcgtcgaaaa ggacgagatc gtgatccgcg ccacgctcga tgcgagcgcg
      481 cgccgcggca ccgtgcgggc cgccatgctc atcgatgccc ctcccgccgt ggtgttcgag
      541 tcgatgaccc gctgtgcgga cgctgtccgg tacgtgccgc accttcgcct gtgccgggtt
      601 cgcgaccgtg ccgccgatgg aagctgggcg ctggtcgaac aggaaatcga tttcggctgg
      661 tacgcgccgc ggctccgtta cacattccgt gccgacttcg tcaccgacca gagcatcgcg
      721 tttcgccagg tcagcggtga cttcaaggcc aatgaaggcg tctgggagtt cgagccggcc
      781 gaggacggcg cccggacgct gctgcgatat cgcgtctata tcgaccctcc gggatatgtc
      841 ccgaactggc tggcgcgttc gaccttcaag cgggagctgc cgcagatgct ggtcgacctg
      901 cgccgctttt gtgaggcgga gcaggcgctg cgcgcccaag caaacacttc tcctgaagga
      961 ctctctccat agactgattg cagaccatgt ggcgcaaccg gcaagatgcc ggcccccatg
     1021 agagccgcca tcgatcgcaa gttcgaacaa acactggcgg cgctgatcaa cagcggcgag
     1081 cccggaatca ttcatggcgg tcgcaagggc atcgaaaggg aatcgctgcg ggtcacgcct
     1141 gacggccgca tcgcgcagac gccacatcca cgcgcccttg gctcggcgct caccaacgaa
     1201 aacatcacca ccgactattc cgaagcgctg atcgagctgg tctcgcctac gttccgcacc
     1261 agctgggaac tgctgcaata cctgctcgac ctgcaccagt tcgtctatca gcacctgggc
     1321 gacgaactgc tctgggcgac ctcgatgccg tccatcatcg atggcgacga gagcatcccc
     1381 atcgcgcaat tcggcacatc gaacgtcggt cgcatgaaaa ccgtctatcg caacgggctc
     1441 ggcgtgcgct acggacgagt gatgcaggcc atctccggcg tgcatttcaa ctattcgttc
     1501 ccggagcaaa tgtgggattc ctgggcggac atcgtgcaat cgcgcgagcg cggccagtcg
     1561 ttcatctcgg atcgctattt ccacctgctg cgcaattacc ggcgccacgg ctggatcgtg
     1621 ctgtacctgt ttggtgtttc gccggtggtg tgcaattcct tcctgcgcgg ccgcaacgtg
     1681 acgctgccgc gcctcgggaa ggacacttcg tacgagccgt acgccacgag cctgcgcatg
     1741 agcgacttgg gctaccggaa ccgcaaccag gccgggcttt ccgtgtcggt caacagcctc
     1801 gacgaatacg tgcgcgacct gtcgcgcgcc atcagcacac cgcacgaacc gtacgagcag
     1861 ttaggcgtga aggtcggcga tgaatatcgc cagctgaacg ccaacatcct gcagatcgag
     1921 aacgagtact acagtttcat ccggccaaaa cgtgtggcgc gttcgggcga gcgtccgacc
     1981 aaggctctgc ggcgggccgg cgtcgaatac gtcgaggtgc gcgcgctcga cgtgagcgcc
     2041 ttcgatccgg tgggcgtgaa ccagaacaag atgcgtttcc tcgagatgtt cctcgcgctg
     2101 tgtctgatga aagactcaca gcccatcggt gtttcggaac aggacacgct ggacgccaat
     2161 catgtgacgg tagcgcgccg cggtcgtgag ccgggtctga cattgcaacg ggatggcaga
     2221 ggcgtatcca tgtccgcctg ggcgcgcgag ctcatcgagt ccatgcaggg cattgccgag
     2281 gtcttcgatc gcggcgatcc ggccaagccg ttctcggccg ccctggccat gcaggccgca
     2341 aaggtgcagg acgtggccct tactccctcg gcacgcatgc tcgcggagct cgaaaccacg
     2401 ggcgaatcct tctttgagct ggggctgcgc atgtcgcgcc ttcataagga gtacttcctg
     2461 gcgttgtacc cccccaatgt ggagcgtcag gctgaattca gggcggcggc ggacgagtcc
     2521 ctcgccgccc agaaggcagt ggaagccgcc gatagaatga gttttgaaca gtatttgtcc
     2581 gaatacgctg ccggctagaa aactgtgacg cacttcaagg tcttgacata tccataaaat
     2641 cttgttctcg aagcctattt tttaacattt ggcgtactag aatccctgcc cagaagaccg
     2701 aagggtccat cagatgttca gggaatgaaa tgaaaaccaa gaatcagacc gagcaatacc
     2761 tgctcgagtt ggcggtagca acagcagcga acgaagatgg aatttctccg ccgacgcgcg
     2821 acggcgtgtc agcgactcgc aaccaggcat ggagcccgct ggaagtgtgg cgcactcgag
     2881 tgaagacgcc cgcaccaaca cgcacggagt cagcccagat ccagggctga tgcgcacgcg
     2941 gcttgcgccg cggctgccga acacatacaa agcagtctca tcttcaggga ccctgcacgt
     3001 atggcgccca cgaaaaattc gacgccaagc acatcggacg catcgcgtct gcttacgaag
     3061 ggtttccgtt ttctccgttt cgacgaaccg ctcgaaaccg aattccgcac cgagcatcgt
     3121 tcgaggctcc aggtctggag ccgcatcgcc atcatcgtct cggcctgcac ggtcatcggc
     3181 ttcgccattc tcgatcactt cgtgctctcc gcagagcatt cgcgcatcac gaacatggtg
     3241 cgcttcggca tccacgttcc ctgcgtcctc atcatgctgc tgctcacctc gaagcgtttt
     3301 tacaaccgct ggtatgagct cggcatcagc ttcgtggcgc ccatgttcgg cattggcaca
     3361 gtcatcatgt ccgcgttctc gccggccaac gaagttccgc tggtgggtgg gcgcctgctg
     3421 ctcgcatcct tcttcttcta tttcatgctc ggcctcccgc tgcgagcggc ggcacgcgcc
     3481 aacctcatcg tgtttgccgc gctggtggtc gcgtttctcg cgggcaccat gccgacctat
     3541 acggccgcct acctcatgtt cacgttgttc acggcgaacg tcatcggtat cgccggttcg
     3601 tacgcgctgg agcatgcgaa tcgcacggcc tttctcgaac accgccagct caccgaagtg
     3661 gccactcatg acggcctgac gacgctgctc aatcgcgccg cgttcgaaga ccagattcgc
     3721 cgcgtctggc agcaggcgca gcgcgaccgg cagacgatct ccgtcatcat gatcgacatc
     3781 gattacttca aggcctacaa cgaccgttat ggccacgtcg ccggcgacga ctgcctgcgc
     3841 cgcgtttcca gcgcgctgcg tgacgccgcg cggcgccgac cgctcgattt cgtcgcgcgt
     3901 tatggcggag aagaactcgt ggccgtgctg tacggcgcgg acaagtccta tggcgagagc
     3961 atttcgcgca gtctcctgac cgcggtgcgc gagttgcgta tcccgcacgc caattcggaa
     4021 acgcagccgt acgtgaccgt cagcgtaggc gtggtggccg tggatgctta ccgtctcgcg
     4081 acgcacgacg cggccgtggc gctagccgac caggccctgt atgccgccaa gcaccagggg
     4141 cgcgaccggt acgtggcgct cgagcacagc atccagaagg tcgacccggg tgccacgact
     4201 gttctgccgg tccgcgagtc cgcgtcattt cgcgaagttt ccgcgggcta gctagcggtg
     4261 ccgggccggg aaaccgggaa aactcggtca tcgcgcgcgg tacaaagttt tcccggtttc
     4321 ccggcccggc accgtcttag cggtagatcg ccacctcgtt cctgccgctc gacgtccgca
     4381 cgccgcggaa catcccctcg ctgttgaaat cgagcacgac ctcgccacgc gagtcgatcg
     4441 caatgactcc gccattgcca cccagatcct tcatcttgcc gagcacgttc gcgaccgctt
     4501 gctcgagcgg cagctcgagg tactccacct gtgcgcagat gtcgtgcgcg accgcggcac
     4561 ggatgaaata ttcgccgtgc ccggtggccg acaccgcgca gcttgcgttg tccgcgtagg
     4621 tgccggcgcc gatgatgggg gagtcgccga cgcggcccca gcgcttgccc gtcatgcctc
     4681 cggtggaggt cgcggcggcc agatttccgg acgcatcgag cgccacagcg cccacggtgc
     4741 cgaaagcgat gaggtcgttt tccttctccg ccgtgccgcc cagcaggcga tgcagctgct
     4801 gctgccttac ctgcgtccgg aagtattcgt tggaaaccat ctccacgcct tgcaggcgag
     4861 cgaattcctc ggcgccctcg ccaaccagca tgacgtgagg cgagcggtcc atgaccagtc
     4921 gcgcgagcgc gatggggttc ttcacgtgcc gcaggcccgt gaccgcgcca gcacccaagg
     4981 tgccgccatc catgatcgac gcgtctaact ccgccaggcc atcgcgcgtg agcaccgccc
     5041 cgcgtcccgc gttgaacagc ggatcatctt ccagggcaca gaccgccgcg atcgccccgt
     5101 ccaggctcgt accgccgcgc tcgagcacgg aataacccgc atcgagggcg gcgctgaggc
     5161 cgtcgctgta ggcgcgttcg tgttctgccg atgtctcgct accggacaat gtgccggcgc
     5221 cgccgtgaat cgcgattgcg aacatgtttt tctccgacaa tccgcacaac tgaagggcat
     5281 gcgaccgtta tcagcaccga aattgacgcc ggaaaaagac gccgatcccg atacaattcc
     5341 gcgtccgggg aatcatatta gaagaagcca tccatgctga ctcgcatccg cagcctgctc
     5401 gtcgccaatc gcggtgaaat cgcgatccgc gtcatgcgcg ctgccaatga actgggtgtc
     5461 cgcaccatcg cgatttactc gcaggaagac cgcttttccc tgcatagaac caaggcggac
     5521 gaggcttacc tggtggggcg cggcaaggcg ccggtggatg cctacctcga catcgaagac
     5581 atcctgcgga ttgcggtcga agccaaggcc gacgccattc atcccgggta tggatttctc
     5641 tccgagaacc cggacttcgt cgacgcctgc aaccgcgaag gcgtgatttt cgtcgggccc
     5701 acgcccgaga tcatgcgcac actcggcaac aaggtggcgg cgcgcaatct agctatttcc
     5761 gcgaaggtcc cggtcatgcc ggcgacgccg ccgctgccgg ccgacatcga ggaatccaag
     5821 aaacaggccg cgatcgtcgg ctatcccgtc atgctcaagg cgagttgggg tggtggcggc
     5881 cgcggcatgc gcgtcatcga aagcgatgcg caattgacgg aactgctgcc cgtggcgcgc
     5941 cgcgaagcca aggcaggttt cggcaacgac gaggtatatc tcgagaagct ggtgcgcaag
     6001 gcgcggcaca tcgaagtgca gatcctgggt gatacccacg ggaatctcgt gcacctcttc
     6061 gagcgcgact gcaccgtgca acgccgcaac cagaaagtgg tcgagcgcgc gcccgcggcg
     6121 ttcctcaccg acgcgcaacg caatgagctc tgcggttatg cgctgcagat cggccgcgcg
     6181 gtcaattacc gcaacgccgg caccgtggaa ttcctgcagg acgccgagac cggcaagttc
     6241 tatttcatcg aagtgaaccc gcgcatccag gtggaacaca cggtgaccga ggccgtgacc
     6301 aacatcgacc tcgtgaaggc gcagatccgc atcgccgacg gtgcaaagat cggtgaagag
     6361 gcaagcggtg ttccgaagca ggcagacatt cacctgacct cgcacgcgct gcaatgccgt
     6421 gtcaccaccg aagatcccga gaacggcttc acgcccgact acggcaagat cacggcgtac
     6481 cgctcgccag cgggtttcgg catccgtctc gacgcgggca ccgcctatgc cggcgccatt
     6541 ctcacccgtt cgtacgactc catgctcgtg aaggtgacgg cgtgggcgcc gaacccggcg
     6601 gaagccatcg cgcgcatgca ccgcgcattg tgggagttcc gcatccgtgg cgtgtccacc
     6661 aacctgcgct ttctcgacca ggtgatcacg catccgaagt tcgtgaacgc cgactacacc
     6721 accaagttca tcgacgagac gcccgagctc ttcaagtggg agaagaagcg cgaccgtgcc
     6781 acgcgcatcc tctcgttcat cggcgagacc atcgtcaatg gcaaccccga agtgaagggg
     6841 cgccagaagc cgaagagcat ggtgcttgcg cgcctgccca aggtgaacgt ggcgaagacc
     6901 ccgccgccgg gcacgaagca gaagctcgac gagctcggcg ccaaaggctt cgcgaagtgg
     6961 atgctcgcgc agaagcaggt gctgatcacc gatacgacca tgcgtgatgc gcaccagtcg
     7021 ctgctggcga cgcgcatgcg ccagcccgac atggcggcca tcgcgccgta ctacgcgcac
     7081 atgcttccgc agctcttctc ggtcgagtgc tggggcggcg ccaccttcga cgtcgcgatg
     7141 cgcttccttc gtgaagatcc gtggtcgcgg ctcgaacagc tgcgcacgct catgcccaac
     7201 ctgctgttgc agatgctgct gcgttcctcg aacgccgttg gctataccaa ctacccggac
     7261 aacgtggtac gcgcgttcgt gaagcaggcg gcggtatccg gcgtcgacgt gttccgcgtg
     7321 ttcgactcgc tcaactgggt ggaaaacatg cgcgtcgcca tcgacgccgt gctcgagacc
     7381 ggcaagctgt gcgaagcggc catctgttat tcgggcaacc tctcgtcgcc gaacgagacc
     7441 aggtacacgc tcgactacta cctcgagatg ggccggaagc tgaaggacgc cggcacccac
     7501 atcctcggca tcaaggacat ggcgggcctt tgccagccca acgccgcgta cacgctggtg
     7561 aaagcgctca aggaagaact caaactaccc gtccacttcc acacgcacga cacgtccggc
     7621 atcgccgcgg cgagcgtgct cgcggcggtg gccgccggct gcgacgccgt cgatggcgcc
     7681 atcgatgcga tgtcgggctt cacgtcgcag ccgaatctcg gctccatcat cgaagcgctg
     7741 cgctacagcg aacgggatcc cggcatcgac cacgcgtcgg tgcgcgccat ctcgatgtac
     7801 tgggagcagg tgcgcaagaa ttacctcgcg ttcgagagcg acatgcgcgc gggcgcgtcc
     7861 gaggtgtacg tccacgcgat gcccggcggg cagtacacga atctcaagga gcaggcgcgg
     7921 tcggtcggcc tcgacgattc ccgctggccc gaggtgtcac aggcctatgc cgacgtgaac
     7981 cagatgttcg gcaacatcgt gaaggtcact ccgtcgtcca aggtcgtcgg cgacatggcg
     8041 atcctcatgg tgtcgtcggg cctcaccaaa gagcaggtcg tcgacccgaa cttcgaagtc
     8101 gccttccccg agtcggtcgt gcagatgatg cgcggcgacc tgggccgccc cgagggcggc
     8161 tggcccgccg gcatccagaa aaaggttctc aaggaccaga agccgctcga acagcgtccc
     8221 ggtgccctga tgccgccgct cgacctcgtg gcggagcgca ggaagctcga tgagaagtta
     8281 ggtcgcgccg caaccgacac gcagttcgcg tcctatctca tgtaccccag ggtgttcctc
     8341 gactacgcga aggaccgcac cgcgtacggc gactgcgcca tcctgcccac acctgtcttc
     8401 ttctacggca tggagccggg cgatgaggtg agcgtcgaca tcgagcgtgg caagacgctc
     8461 atcgtgcggt tcgtcgcgct gtccgaagtg cgtgacgacg gcacgcgcca ggtgtttttc
     8521 gaactgaacg gccagccgcg ttccatcgtc gtcaccgatc gctcgcaggt ggcaaagcgg
     8581 ccgccgcagc gcaagatgga gccgggcaat gccaggcatg tcggtgcacc gatgccggga
     8641 acgatcgcga cggtgaaagc catcgtcggc cagaaagtcg ccaagggcga cctgctgctc
     8701 acgatggagg ccatgaaaat ggaaaccagc gtgcgcgcgg aggcggacgg caccgtggcc
     8761 gaggtgctgg tgaagccggg cgtgcaggtg gacgcgaagg acttgttagt cgttctttcc
     8821 tgagcgcgga ctcacgattc gtatcctcgg cgaggccggg aagcgccagc tagtgacgcg
     8881 ccagcccagg cagcgagtcc agccactgcg tccggcactg gcgcacgcga tgcaggtcgt
     8941 ccacgccaag ccagcaggtg cggaacacat agcggacgtg ttgatgcccg gtatcgacca
     9001 cgaaacccca gtcttaggcg aggtcaacga tatcgataga gtcgcacctt ctggatctgg
     9061 aacggcccag gcggcaggcg gatgtgccgg ccctggtgcg tctgatctcc gttgagccgg
     9121 cggccgggag tccattttcc cttttcgtcg aactcgcctt cttcggccac atcgatgccg
     9181 acgagcggcg ggccgtcgcc gacgccgcgc atcgtcacag tcatgccttg accggcgaac
     9241 cagaaatcct cgtcaccggc atgaacaacg agcccgccgt gatccgcgat cgtctgcttc
     9301 tcgcgcggcg tccatggatc gatgaaggtc accgtgaact cgatgccgcc cagcatcttt
     9361 ttcaccggcg aatcaatcac agatccatcc tcattgattg tggcccgaaa tccacccaat
     9421 cggccacggc cgcgggcgtc gagcaggacc ggcgtcagct gcttcaatac ctcgtatgac
     9481 tgcgacagcg cattcggagc atcgcccagc gattcgatcg agaacggcga aaagcccagg
     9541 gaatcaagcg caccgaatga atagaaggcg ttggccgaga cctgcgaatt cgtcgcgttg
     9601 ttcgcctcgg gaatgaacag cacgttgtcc ggtcgattga agcgcgcggc gagctgcgca
     9661 aaattcggga agtagatgtc gggtgaaagc atgtcggtgg tcggcgtgcc cgctttccag
     9721 acatcgatga ggtgcggcag cgggccacca ctcggatact cgccaggctt tcggccggtg
     9781 cggttgagcg ccacgtttac gtacagcgga atgtcgtaac gcttcttgcc ggccaccacg
     9841 agcgcctcga cgaatcgcgc gtagtgccag gcggtgaaaa cttcggctcc cgcttcttca
     9901 ttgccaaaca cttcagccca ggtgccggat gtcctggcgc cgtgcttgct ccagagagcc
     9961 tgcagttcgg gttcgagctt ttcgcgtttg ctcagcgagc gaatcaactc aggcggtacc
    10021 ggcccggcaa acaatttttc tgccggcgcg ccgcgctcgc gcgcaatcgg caacattccg
    10081 atctcattct ccacctgcac catgagcacg gtgttcttct cgccgtcgac gcgcttcaga
    10141 tattccatga atgcgccgaa cgcctgcgcg tcggcgtcgc gcgtgttcgt agagaacgcc
    10201 gacaggattt ccacgctcga tccgttggca agttgcgcgc gcgggaaacg attctgatcg
    10261 cgcttgaccc acgatgggac gtaggtggac atgctgttct tccacgcgcc gaaccagagg
    10321 atcacgagct tgagatcgtg cgcgcgagca tcgcgcagca gctcatcgac cgaggtccag
    10381 tcgaatttcc cttcgtgggg ctcgatgagc tcccacgaaa ccggcgccag cacggtattg
    10441 agattcatcg ccttgagccg cggccaatgc gggcgcatgt agttggcgct cgagctgctc
    10501 gaattgccga gctcgccgcc caggatcagg aacggtttgc cgtcgacgat gagttgctgc
    10561 gtgctgcctt gtttcgcgag atgaggtggc gcagcttgcg cggccatcgc ggtggcgagt
    10621 gccgcggcga gaacgatgct gagagctttc acgcgccgga tgttaacaag cgcgtgcatg
    10681 ggctctgcga gaagagctcc gcggcccagt cgacgaacac gcgtaccttg gtcgacaggt
    10741 ggcgattcgc gggatagagc acgtgcacgg gcagcggctc gggctgccaa tcctgcagca
    10801 cgggcacgag ctcctgcgtt gcgttgctgg cggagaccat gaacgagagc agctggccca
    10861 ggccggtgcc agcccgcaac gatgcgaaat gggcgccgct atcattgacg gcgactcgag
    10921 ggctcggggc gatttcgatg cgcttgccgt cgcgctcgaa gatcagagga cgcatgcgac
    10981 cgctgcgcgg aaagaaatag cccgcgagca cgtggccgga ttccaggtcc gtggggtgcg
    11041 tgggtttcgg atgccgcgcg aaataggagg gcgtcgcgca ggtcagccag cgcatgtcgc
    11101 cgatgtgcct tgccacgagt gacggttcca gcaaggggcc accgcggatc acgcagtcga
    11161 cgttgtcacc gatgagatcg gcaggccggt cgctgacgcc gcattcgata tcgatctccg
    11221 ggtatttcgc gaggaatccg ggcagcgcgg gcaagatcag gtagtacgac atcgccgccc
    11281 ccgcgtcgat gcgcagccgc ccgcgcggcc gctccttggc atgcgtgacg ctcgattcga
    11341 tgtcctcgag atcgcctaac aacctcacca cgcgctcgta gtacgcggac ccatcgggtg
    11401 tcacggtcac gcggcgcgtg gtgcggttca ggagcttgat gcgcaggtgg ctctccagcc
    11461 cctgaacgag cttggtgacc gtgggtttgg gcatgcgcag tgagttggct gcgttggtga
    11521 acgtgccggc ctcgaccacg cggacgaaca ccctcatggc cagcatctgg ttgaaattct
    11581 gggcggcttc catggccggc attattccct attgggaata atgttgtaac aactgccgta
    11641 tttatcggtg tgccgatagc taatagcctc ctcccaacgc gaaaacacgc gccaaaccca
    11701 tcaactcgag gagaaagtca tgaacaagaa gctcacgggt aaagccgccc tggtcacggg
    11761 tggctcgcgc ggcatcggcg ccgccattgc ccgatcactc gccgaggacg gcgcg
//
LOCUS       DASQJS010000421         4089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1217632, whole genome shotgun sequence.
ACCESSION   DASQJS010000421 DASQJS010000000
VERSION     DASQJS010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217632"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VEW08_16630"
     CDS             complement(<1..302)
                     /locus_tag="VEW08_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42418.1"
                     /translation="MSAKPDYVVSKLTRPTGGAATGGFVINLTSSTTPMALSQPKDPT
                     LAQFSFFVSRRLEDGRERFRLHMGYFDSMQAAEEMLAVVREVYPGAWASEAPGKKL"
     gene            complement(520..1029)
                     /locus_tag="VEW08_16635"
     CDS             complement(520..1029)
                     /locus_tag="VEW08_16635"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HYJ42419.1"
                     /translation="MRNVPILLLLLFAAGCTSAPEPRPQPAPTREWDGKGGAAGMRNR
                     GEALADFALRLRGTPYRYGGATLDGFDCSGLVFYAHRQLGLAVPRTSRDQAEEALSIK
                     PRKLQRGDLVFFKIGSGKVNHVGIYIGEHQFVHAPGAGKPVTINSLDDEFYADRFFSA
                     GRYWNRLPN"
     gene            complement(1066..3606)
                     /locus_tag="VEW08_16640"
     CDS             complement(1066..3606)
                     /locus_tag="VEW08_16640"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ42420.1"
                     /translation="MSGNPYNSILRSGLTRRLWLIITLAMLIPVSIAMLSRWFEAEER
                     HARLQNQELSALSRDKASSLMFSERVVPEDFASGLDGRFLVVLDGSGAARYRSSPVPE
                     ELVQLFGRRAPNAVDAPGGTAILAWFAAGREWRGALTYLPPIIPGNPNTADTIVVFAP
                     EAAFGMTAAGLVPTALGLLVLTVVVAFAVAAIVSERYLPPLRALQRGLTRLRERRFET
                     LPRSTVDEFISLEREFNATSLSLQRDWRAFEVLGEVDRALLAASEIDRALDTVLPKFR
                     ELTRAQCVGVILLDPTAHAHGRLFMSALGADELPVQRVTFDSAMIATVRESPEGLTIA
                     RVEESRHAFLLSMRDVGAEFFWLWPVVDEERLSALLVVGYHGEPSRDPAVADYGAAFA
                     ERLRAALSNSARDERLYRQAHYDPLTQLPNRALFRDRLAQELAAAATGLSRGALMYVD
                     LDHFKRVNDTLGHSAGDQLLSIVAHRLKACTKEGDTVARLGGDEFTVLLRNVGDAETA
                     RQIADRVIRSLALPASLGGRDYQMRASVGVTLFPDDGVDLEDVIRQADLAMYRAKAQG
                     RGRAIFFERDMVQRRASFTDSGLHRALRRRELSLFYQPQFTLADNRLCGVEALLRWQT
                     RYDGIKLPGEFIPAAEHSGLITDIGGFVLDSACSQYAEWARAGIAPRMFSVNVSVQQL
                     KDSNFAAVVRGALQQHGISPANLELEFVESALADAEVEEPLRALASLGVKLALDDFGT
                     GYSSLNYLRRYPVNTVKLDRSFLEEVPQNDSAGALVESVITMAHTLGKRVTAEGVETG
                     GQLEFLRSRGCEAAQGFYLARPMSATALSEILESRRPGSEIGDERAAG"
     gene            complement(3732..>4089)
                     /locus_tag="VEW08_16645"
     CDS             complement(3732..>4089)
                     /locus_tag="VEW08_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42421.1"
                     /translation="TSKKFYLRTKGEMEEAVTDLGFASVDILQPSLLLGPRTTMRPLE
                     IAGGIFAPLINPLLTGARESLRAIPAETVARAMLGASRRGARGTYRYTYGAIRQLAEI
                     RSAQSLAPQSPKKKPA"
BASE COUNT          770 a         1298 c         1331 g          690 t
ORIGIN      
        1 ttcagtttct tgccgggcgc ttcgctggcc catgcgccgg gataaacctc gcggacgacc
       61 gcgagcattt cttcggcggc ctgcatggaa tcgaagtagc ccatgtgcag gcggaatcgc
      121 tcgcggccat cttcgaggcg gcggctgacg aagaatgaaa attgagcgag ggtcggatct
      181 ttgggctgcg acaacgccat gggcgtggtc gacgacgtca gattaattac aaagccgccc
      241 gtcgcggctc cgccggtggg ccgcgtcagc ttgctgacga cgtagtctgg cttggcactc
      301 atgtttaaaa cgttccccga aagatcgaga ccgaaccgcc tcccttcccg aaagcgatcg
      361 ttatttccac tcactttgga gtgtgcgaac caggggatat acggtcaatc ggcgaactga
      421 gcacgctgtc gcagttcgca gacctaaata ttcaatccct tagtgaaaat tcgacataac
      481 ggtgtgggat tccatgctgc aaagcgttat cgagcggcat cagttgggca ggcggttcca
      541 gtaccgtcca gccgagaaaa accggtccgc gtagaactcg tcgtcgagtg agttgatggt
      601 caccggcttg ccggcgcccg gggcatgcac gaactggtgt tcgccgatgt agatgccgac
      661 gtgattgacc ttgccgctgc cgatcttgaa gaacaccagg tccccgcgct gaagtttgcg
      721 cggcttgatg ctcagggctt cctcggcctg gtcgcgcgaa gtacgcggca cggcgagacc
      781 cagctgacgg tgcgcgtaga acaccaggcc gctgcagtcg aatccatcga gggtcgcacc
      841 accatagcga tagggtgtgc cgcgcagccg aagcgcgaag tctgcgagcg cctcgccacg
      901 attgcgcatg ccggcggcgc cgcccttgcc gtcccattcg cgcgtcggcg caggctgcgg
      961 acgcggttca ggtgcagagg tgcaacccgc cgcaaacagc agaagcagca gaatgggaac
     1021 attcctcatg gcagtgaaaa tgagggatat ccccttaacc cgcccttaac ccgccgccct
     1081 ttcatcgccg atctcggaac cgggcctgcg ggactcgagg atttccgaga gcgcggtggc
     1141 cgacatgggc cgtgccagat aaaagccctg ggccgcctcg cagccgcgcg aacgcaggaa
     1201 ttcgagctgc ccgcccgttt ccacgccttc ggccgtcacg cgttttccga gcgtatgcgc
     1261 catggtgatg accgattcga ccagtgcacc ggccgaatcg ttctgtggga cttcttccag
     1321 aaagctgcga tccagtttta ccgtgttgac cgggtaacgc cgcaaatagt tgagcgacga
     1381 atatccggtg ccgaaatcgt cgagcgcgag tttcacgccc aggctggcca gcgcgcgcag
     1441 cggttcctcg acctcggcat cggccagcgc cgattccacg aactcgagct cgaggttggc
     1501 gggggaaatg ccgtgctgct gaagcgcgcc ccggaccacc gctgcgaaat tgctgtcctt
     1561 gagctgctgc accgagacgt tcaccgagaa catgcgtggc gcgatgccgg cgcgcgccca
     1621 ttcggcgtac tgcgaacagg ctgaatcgag cacgaacccg ccgatgtcgg tgatgagccc
     1681 ggaatgttcg gccgcgggaa tgaattctcc gggcagcttg atgccgtcat accgggtctg
     1741 ccagcgcagc aacgcttcga caccacacag gcgattgtcg gcgagcgtga attgcggctg
     1801 gtagaacagg gacagctcgc gccggcgcag tgcgcgatgc agcccgctgt cggtgaagct
     1861 cgcgcgcctc tgcaccatgt cgcgctcgaa gaagatggcg cgcccacgac cctgcgcctt
     1921 ggcgcgatac atggcgagat cggcctgccg gatcacgtcc tcgagatcca cgccgtcatc
     1981 gggaaacagg gtgacaccaa cgcttgcgcg catctggtag tcgcgtccgc cgagactggc
     2041 cggcagcgcc agtgagcgga tcacgcggtc ggcgatctgg cgtgcggtct ccgcgtcacc
     2101 gacatttcgc aacagcaccg tgaattcatc gccgccgagg cgcgccaccg tgtcgccctc
     2161 tttcgtgcat gccttgaggc gatgcgcgac gatcgacagc aactgatcgc ccgcgctgtg
     2221 gcccagcgtg tcgttgacgc gcttgaagtg atcgagatcc acgtacatga gcgcgccgcg
     2281 cgagaggccg gtggccgcgg cggccaactc ctgcgcgagc cggtcgcgaa acaacgcgcg
     2341 gttcggaagc tgggtgagcg gatcgtagtg cgcctgccga tagagccgct cgtcgcgcgc
     2401 gctgttcgag agagccgcgc gcagacgctc ggcgaatgcg gcgccgtagt cggccaccgc
     2461 cggatcgcgc gatggttctc cgtgataccc aacgacaagc agagcgctca gtcgctcttc
     2521 gtcgaccacc ggccagagcc agaagaactc cgcaccgacg tcacgcatcg acaagaggaa
     2581 tgcgtggcgg gattcttcga cgcgcgcaat ggtgaggcct tcgggcgact cgcgcaccgt
     2641 ggctatcatc gcggagtcga aggtcacgcg ctgcaccggc aattcgtcgg cgccgagcgc
     2701 cgacatgaac aggcggccgt gtgcgtgcgc ggtgggatcg agcaggatca cgcccacgca
     2761 ctgggcccgt gtcaattcac gaaacttggg cagcaccgtg tcgagcgcgc gatcgatctc
     2821 gctggcggcg agcaatgcgc ggtccacttc acccagcacc tcgaaggcac gccagtcgcg
     2881 ctgcagggag aggctggtgg cattgaactc ccgctcgagc gagatgaatt catcgacggt
     2941 actgcgcggc agggtttcga agcggcgttc gcgcaatcgg gtgagcccgc gctgcagcgc
     3001 gcgcaacggc ggcaggtagc gttcgctgac gatggccgca accgcgaagg ccacgacgac
     3061 ggtgagcacc agcagaccaa gcgccgtggg aaccagcccc gcagccgtca tgccgaaggc
     3121 cgcctcgggt gcaaacacca cgatggtgtc agcggtgttc ggattgccgg gaatgatcgg
     3181 cggcaggtac gtcaatgcgc cgcgccactc gcggcccgcc gcgaaccagg cgaggatcgc
     3241 cgtgcctccg ggcgcgtcga cggcattcgg cgcgcggcgt ccaaaaagtt gcacgagttc
     3301 ttcgggcacc gggctgctgc gataacgcgc cgcgcccgaa ccatcgagca ccacgagaaa
     3361 acgcccgtcg agaccgctgg cgaaatcttc cggcacgact cgctcgctga acatcagcga
     3421 cgacgccttg tcgcgagaga gagcggagag ttcctggttc tgcagccgcg catgccgttc
     3481 ctcggcttcg aaccagcgcg acaacatggc gatgctgacg ggaatgagca tcgcgagcgt
     3541 aatgatcagc cacaggcggc gtgtgagacc gctgcgcaag atcgagttgt atggattgcc
     3601 gctcatccac ttcccatggg ccggcgttgc gcatctgcaa cgcaaaccag gcattcttga
     3661 tatcgctgcg actttacgga atgtggagcc gcgcaaatgc gcgccgcgtc acgaatgcgg
     3721 ttgcaccgta ttcaggcagg cttcttcttg ggcgattgtg gcgcgaggga ttgcgccgag
     3781 cggatctccg caagctggcg gatggccccg taggtgtagc gatacgtgcc gcgcgcacca
     3841 cgccgcgatg caccgagcat cgcccgggcc acggtctccg cagggatggc acgcagggat
     3901 tcacgcgcgc ccgtgagaag cgggttgatg agcggagcga agatgccgcc agcgatctcc
     3961 agcggccgca tcgtcgtgcg cggacccaac agcagactcg gctgcaggat gtcgaccgag
     4021 gcgaacccga ggtcggtcac cgcctcttcc atctcgccct tggtgcgcag gtagaacttc
     4081 ttcgacgtc
//
LOCUS       DASQJS010000422         5397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2162580, whole genome shotgun sequence.
ACCESSION   DASQJS010000422 DASQJS010000000
VERSION     DASQJS010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162580"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VEW08_16650"
     CDS             <1..464
                     /locus_tag="VEW08_16650"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HYJ42422.1"
                     /translation="ASYDVPFHHLPVTATTKDAQERKIMALIEEEQIDCVVLARYMQV
                     LSPDLCTGLSGRAINIHHSFLPSFKGARPYHQAYERGVKIIGATAHYVTADLDEGPII
                     EQAVERVGHEHTPERFQEIGRDLEAIVLARAVRWHAERRILLDGQRSVILS"
     gene            complement(487..1161)
                     /locus_tag="VEW08_16655"
     CDS             complement(487..1161)
                     /locus_tag="VEW08_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42423.1"
                     /translation="MTHNKALLLAISSAVALALSATSFAATKSKICLVLPRAQLGQGT
                     SGADVAEPVRATLTAYLGGPATELVPLTARIPIQIDAETAELGCAYVLYSSVTQKKGG
                     GFSKLLGAAAPLAQVGTAMTGMGGSYSAIMATQAVATVATNAAAKSAQDEAMEALGGA
                     AKSNIRKGDTITLEFKLMQPGNATPVAASSAVAKATQNGEDLVSPLIEQAATQVLTAI
                     TQPPAP"
     gene            1290..2843
                     /locus_tag="VEW08_16660"
     CDS             1290..2843
                     /locus_tag="VEW08_16660"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HYJ42424.1"
                     /translation="MIRERSLAWVLLLALPLTLGACAQLQPRGELPMELATPIATESG
                     APLDRAFMPHEAAHAGQSAFRLLIDGTEAFVVRMQSARMAVRSLDVQTYIWHADLTGR
                     FLAHQLLDSADRGVRVRLLLDDMDARAKNAHFAALAAHPNIEVRVFNPFASRNGTLSL
                     VSEGARNFKRINRRMHNKTWIADNRLAIVGGRNLGDEYFGASDEVNFVDLDFGMVGPV
                     VRDASASFDRYWNSPSAYPLELLDPDAVDAKILGKLRDRLAAFARESAGGRYAVALRE
                     DAAIQRLLAGNWPMEWSGNYRFIADDPLKISMKKRDAHRAAVSAVLLPAMRDARSALD
                     VISPYFVPGDKGSTGLVNTARAGSQVRILTNSLAANDVAAVHGGYSRYRNKLLKGGVL
                     LWELKPFAISEQDKSLFGSSGASLHTKALSIDQKTLFVGSYNLDPRSTWLNCEQGVLV
                     ESPALVTQFRAIFEAQTAGTHAWRVTREGGELHWADDKGVTDKEPQTTFAQRMQAWFA
                     RVLRIDAQL"
     gene            2986..3270
                     /locus_tag="VEW08_16665"
     CDS             2986..3270
                     /locus_tag="VEW08_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42425.1"
                     /translation="MSDDTIKEAGTIQVMLQRLNEFRLPKALDLKKKVDRGEKLDSSD
                     IEFLQTVLADATGAQSLVAKHPELKPLVGKLMGLYSEITTRGLENEQTKG"
     gene            3275..4063
                     /gene="ppk2"
                     /locus_tag="VEW08_16670"
     CDS             3275..4063
                     /gene="ppk2"
                     /locus_tag="VEW08_16670"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HYJ42426.1"
                     /translation="MAKKDDPLGRKDYEEQLRKLHVELVKAQAWIKHKGLKVCIVFEG
                     RDGAGKGGTIKAITDRVSPRVFRVVALTAPTEREKSQMYVQRYLPYLPAAGEVVIFDR
                     SWYNRAGVERVMGFCDEADVKRFLRIVPAVERAIIDSGVILLKYWLEVSPEEQTRRLE
                     ARMQDGRKLWKLSPMDLKSYSRWYDYSRARDEMFVATDTGHAPWYVANSNDKRRVRLN
                     IISHLLERIPYKELKREKIKLPKRQKRGGYRDANYPFKFIKERY"
     gene            4201..>5397
                     /locus_tag="VEW08_16675"
     CDS             4201..>5397
                     /locus_tag="VEW08_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HYJ42427.1"
                     /translation="MPNKESYDDRVIRLFLMAAAIWGVVGMSIGVLAAAQLAWPALNF
                     DVSWLTFSRIRSNHTFGVIFAFGGSALMGTCYYIVQRTGHVRLAFGKLAMFTFWGWQA
                     GCVLAMATMPFGITQAKEYAEPEWFIDLAIAVVWVSFAVVFFGTLARRRIAHIYVSNW
                     YFGALIIAVALLHIVNNLALPVSLTKSYPIYSGAVDAMVQWWYGHNAVAFFLTAGFLG
                     MMYYFVPRQAQQPLWSYRFSIVNFWALISVYMWAGSHHLLYTALPDWVQSVGMVFSLV
                     LLMPSVGSAANGLMTFNGSWHKVRIDPAAKFMVMALVFYAASTIEGSMMAIKSVNSLT
                     HYTDWTVAHVHSGAIGWVAMITMGSLYAMAPRALDRPAVHSTAAMNLHFWLHTSGLLL
                     YVVAMWS"
BASE COUNT          953 a         1691 c         1721 g         1032 t
ORIGIN      
        1 ctgcttccta cgacgtgccc ttccatcatc tgccggtcac ggccacaacg aaggacgcgc
       61 aggagcgcaa aatcatggcg ctgatcgagg aggagcagat cgattgcgtc gtgctcgcgc
      121 gctacatgca ggtgctgtcg cccgatctct gcaccgggct gtcgggacgc gccatcaaca
      181 tccatcactc gtttctgccg agtttcaagg gtgcgcgtcc ataccaccag gcctacgagc
      241 gcggcgtgaa gatcatcggt gccaccgcgc actacgtcac ggcggatctc gatgaagggc
      301 cgatcatcga gcaggcggtc gaacgcgtag gtcatgagca cacgcccgaa cgtttccagg
      361 aaatcggccg cgacctcgaa gccatcgtgc tggcgcgcgc ggtccgctgg catgcggaac
      421 gcagaatcct gctcgatggc cagcgcagcg tcatcctgag ctgaggcctg cctccgtggc
      481 aggttgttag ggcgctggcg gctgcgtgat ggcggtcaat acctgcgtcg ccgcctgctc
      541 gatcaacggt gacaccaggt cttcgccgtt ctgcgtggct ttggcaactg ccgacgacgc
      601 tgctactggc gtcgcattgc caggctgcat cagtttgaat tccagcgtga tcgtgtcgcc
      661 tttcctgatg ttcgacttcg cggcaccgcc gagtgcttcc atggcttcgt cctgcgcgga
      721 cttcgccgcg gcattcgtgg cgacggtggc cacggcctgg gttgccatga ttgcgctgta
      781 gcttccgccc atgccagtca tggccgtccc gacctgcgcc agcggtgcag cggctcccaa
      841 cagcttgctg aagccgccac ctttcttctg cgtgaccgag ctgtacaaca cgtaggcgca
      901 gccgagttcg gccgtctcgg cgtcgatctg gatcggaatg cgggcggtga gtggcacgag
      961 ctccgtggcc ggcccgccca ggtaggcggt cagtgtcgcg cgcaccggct cggcgacatc
     1021 ggctcccgag gtcccctggc cgagctgcgc cctgggcagc acgaggcaga tcttcgactt
     1081 tgtcgcggcg aagctggttg ccgacagcgc gagcgccacg gcagacgaga tggcaagaag
     1141 aagggctttg ttgtgagtca tgttggcttc tccggtacgc gttcctgtct tttgagcgga
     1201 ttcggcgaag gctgcagatc accggaaatc gaaaatgagc cggtgcccgc gtatgctcgg
     1261 gcaacctcga acttcaggct gccgtctcaa tgattcgcga gcgatcgctg gcgtgggtgc
     1321 tcttgctcgc acttcccctg acgctgggcg cctgcgcaca gttgcaaccg cgtggtgaat
     1381 tgccgatgga actcgcaacg ccgatcgcga ccgaaagcgg cgcaccgctc gaccgcgctt
     1441 tcatgccgca cgaagccgct cacgctggcc agtcggcatt ccggttgctg atcgacggta
     1501 ccgaggcctt cgtcgtgcgc atgcaaagcg cgcgcatggc cgtgcgcagc ctcgacgtcc
     1561 agacctacat ctggcacgcg gatctcaccg gcaggttcct cgcccatcag ctgctcgact
     1621 cggcggatcg cggcgtgcgc gtgcgcctgc tgctggatga catggatgca cgcgcaaaga
     1681 acgcgcactt cgccgctttg gccgcgcatc cgaacatcga agtgcgcgtc ttcaatccct
     1741 tcgcctcgcg caacggcacg ctgagcctgg tcagcgaagg tgcgcggaac ttcaagcgca
     1801 tcaaccggcg catgcataac aagacctgga tcgccgacaa ccggctggcc atcgtcggcg
     1861 gccggaatct cggtgacgaa tatttcggcg cgagcgatga agtgaatttc gtcgacctcg
     1921 atttcggaat ggtcgggccg gtcgtccgtg acgcttcggc atcgttcgat cgttactgga
     1981 actcgccgtc ggcctatccg ctggaactgc tcgatcccga cgcagtcgat gccaaaatcc
     2041 tgggcaagct gcgcgaccgc ctggccgcgt ttgcacgcga gtccgccggc ggccgctatg
     2101 cggtggcgct gcgcgaggac gccgccatcc agcgcctgct cgccggcaac tggcccatgg
     2161 aatggtcggg caactaccgg ttcatcgccg acgatccatt gaagatatcg atgaagaaac
     2221 gcgacgcgca tcgcgcggcg gtgagcgccg tgctgctgcc ggcgatgcgt gacgcgcgtt
     2281 cggcgctcga cgtgatttct ccgtatttcg ttccgggcga caagggcagc acggggctcg
     2341 tgaacaccgc gcgcgccggc agccaggtgc gcatcctcac gaattcgctc gccgccaacg
     2401 atgtcgcggc ggttcacggc ggctactccc gttaccggaa caagttgctc aagggcggtg
     2461 tgttgctgtg ggagctgaag cctttcgcga taagcgagca ggacaagagc ctgttcggtt
     2521 cctcgggcgc aagcctgcac accaaggcgc tatccatcga tcagaagacc ttgttcgtgg
     2581 gcagctacaa cctcgatccg cgctcgacct ggctcaactg cgaacagggc gtgctcgtcg
     2641 aaagccccgc gctggtcacg cagtttcggg cgattttcga ggcgcagacc gcgggcacgc
     2701 acgcgtggcg agtcacgcgc gaagggggcg agctccattg ggcggatgac aaaggcgtga
     2761 cggacaagga gccccaaaca actttcgccc agcgaatgca ggcctggttc gcgcgcgtgc
     2821 ttcgcatcga cgcacaactt tgagttagta cagcccctgg cggcgcccgt gggaccaagg
     2881 tctcatttcg gggcgggtcg gcgtggttcg atattggcga cccgagtggc tttcaaacta
     2941 ccgcggtgga tggctgcgcc cctatgtggt cgcgcgggag aaggcatgag cgacgacacg
     3001 atcaaggagg ccgggaccat ccaggtgatg ctgcagcggc tgaatgaatt tcgcctgccg
     3061 aaggcgctcg acctgaagaa gaaggtcgat cgtggggaaa aactggacag cagcgacatc
     3121 gaattcctgc agacagtgtt ggcggacgcc accggcgcgc aaagcctggt cgcgaagcat
     3181 ccggaactca agccgctggt cggaaagctg atgggtcttt acagcgagat caccaccagg
     3241 ggtctcgaaa acgaacagac gaaaggttga agtcatggca aagaaagatg acccgctcgg
     3301 ccgcaaggac tacgaagagc aactcaggaa attgcacgtc gagctcgtca aggcgcaggc
     3361 ctggattaaa cacaagggtc tcaaggtgtg catcgtgttc gagggccgcg atggcgccgg
     3421 caagggcggc accatcaagg ccatcaccga tcgcgtgagt ccgcgcgtgt tccgcgtggt
     3481 ggcgctgacg gcgcccaccg agcgcgaaaa gagccagatg tacgtgcagc gctacctgcc
     3541 gtacctgccc gcggcgggtg aggtcgtgat cttcgatcgc agctggtaca accgcgcggg
     3601 cgtcgagcgc gtcatggggt tctgcgacga ggccgacgtg aagcgcttcc tgcgcatcgt
     3661 gccggcggtc gagcgcgcca tcatcgattc gggcgtgatc ctgctcaagt actggctcga
     3721 ggtgagtcct gaagaacaga cgcgccgcct cgaagcgcgc atgcaggatg gccgaaagct
     3781 ctggaagctc tcaccgatgg atctgaagtc gtacagccgc tggtacgact actcgcgcgc
     3841 gcgcgacgag atgttcgttg cgaccgatac cggccacgct ccctggtacg tggcgaacag
     3901 caacgacaag cggcgcgtgc gtctcaacat catttcgcac ctgctcgagc ggattcccta
     3961 caaggagttg aagcgggaga agataaaact tccgaaacgc cagaaacgtg gcgggtaccg
     4021 cgacgcgaac tatccgttca aattcatcaa ggagcgctat taggggctcc gggtcgagcg
     4081 cggtgggctt acaccgcgga tcggcgcgcg cctataggtt ggaacacgga taggaccaag
     4141 gtctcattcc tgcgagcttt gccggactcg atattcacac ccggtcctcc ccaggcacgc
     4201 atgcccaaca aagaatccta cgacgaccgc gtcattcgac tgttcctgat ggctgcggcc
     4261 atctggggcg tcgtaggcat gtcgattggc gtgctcgccg ccgcgcaatt ggcctggccg
     4321 gcactcaact tcgacgtttc ctggctcaca ttcagccgta tccgctccaa ccacactttc
     4381 ggggtcatct tcgcgttcgg cggttccgcg ctgatgggta cctgctatta catcgtgcag
     4441 cgcaccgggc acgtgcggct ggccttcggc aagctcgcga tgttcacgtt ttggggctgg
     4501 caggcggggt gtgtcctcgc catggccacg atgcccttcg gaatcacgca ggccaaggag
     4561 tacgccgagc ccgagtggtt catcgacctg gctatcgccg tggtgtgggt gtcgttcgcc
     4621 gtggtgttct ttggcacgct cgcgcggcgc cggattgcgc acatatacgt ctcgaactgg
     4681 tacttcggcg cgctgatcat cgccgtggcg ctgctgcaca tcgtcaacaa tctcgcgctg
     4741 ccggtctcgc tgaccaagtc ctaccctatc tactcgggcg ccgtggatgc gatggtgcag
     4801 tggtggtacg gtcacaacgc ggtcgcgttt ttcctcacgg ccggcttcct cggcatgatg
     4861 tattacttcg tgccgcgcca ggcgcagcag ccgttgtgga gctatcgctt ctccatcgtc
     4921 aatttctggg cgctcatctc cgtctacatg tgggccggct cgcaccacct gctgtacacc
     4981 gcgcttccgg actgggtgca gtcggtgggc atggtgttct cgctggtgct gctcatgccg
     5041 agtgtcggtt cggcggccaa cggcctgatg acgttcaacg gttcgtggca caaggtccgc
     5101 atcgaccccg ccgccaagtt catggtcatg gcgctcgtct tctatgccgc ttcgacgatc
     5161 gaaggctcga tgatggcgat caagtcggtg aactcgctca cgcactacac cgactggacc
     5221 gtcgcgcacg tgcactcggg cgccatcggc tgggtggcca tgatcaccat gggctctctg
     5281 tatgcgatgg cgccgcgcgc gctcgatcgt cccgccgtgc attcgacggc ggccatgaac
     5341 ctgcacttct ggctgcacac cagcggactc cttctctatg tggtggcgat gtggtcc
//
LOCUS       DASQJS010000423        10323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4048975, whole genome shotgun sequence.
ACCESSION   DASQJS010000423 DASQJS010000000
VERSION     DASQJS010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4048975"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..677
                     /locus_tag="VEW08_16680"
     CDS             102..677
                     /locus_tag="VEW08_16680"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HYJ42428.1"
                     /translation="MKTNLKPQSAPEGNAFISDLSALRSRAREQMMKGAVTPSFPEAD
                     RKAIVELLNTALATEIVCVLRYKRHYYSTHGIRAKFVSAEFLEHAKEEQAHADQIAER
                     IVQLGEDPDLNPATLLSRSHSEYDEATSLRQMLEADLFAERIAIESYREMINFIGPRD
                     PTSRRMLESILAQEEEHAEDLVDLLEDLPKD"
     gene            679..1386
                     /locus_tag="VEW08_16685"
     CDS             679..1386
                     /locus_tag="VEW08_16685"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYJ42429.1"
                     /translation="MHDADEQPGRELEALVAARTEELSTLSTHLLRRTESERSALAKE
                     LHDELGGLITAAKMDMSWLSAHLRNSLDAESREKLDSVMQMLNQAMMLKRRVVEDLRP
                     SLLDHFGLAVALRSHFDEHCARAGIECVSTLPDDGLELDAVTQLSLFRVAQEALVNII
                     SRGGAKHVEVVIEAEGEGYVMVIGDDGAPADTNLARSMPSARHRMQWAGGSVAVEARA
                     EGNQIRVFVPRSAAGSA"
     gene            complement(1346..1789)
                     /locus_tag="VEW08_16690"
     CDS             complement(1346..1789)
                     /locus_tag="VEW08_16690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ42430.1"
                     /translation="MKRQNSSDTVRANGAPGKPLRILLVEDSPLLRSRLESMLSQYAA
                     FKVTGLASAEAEAVQKLETVPYDAIVVDVELRPGSGIGVIRSARAQNPDKGNGDHVWI
                     IVLTNYDLPTVRERCMAAGADHFLDKMREIDQLMPILQQIAGRKP"
     gene            complement(1876..2490)
                     /locus_tag="VEW08_16695"
     CDS             complement(1876..2490)
                     /locus_tag="VEW08_16695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ42431.1"
                     /translation="MDDHQVVRAGLKTLINGQPDLAVVTEASSGREAMEQLRAHTIDV
                     LLLDLSLPDVSGLDVLKHAKAHYESVGVLILSAFPEAQYGLNVLRGGASGFVGKGTEE
                     SELFRAIRTVARGGRYVGPELADLLVAGVRGDPNAPLHSILSEREFQIFCKLAQGESV
                     SEIANRLFLSVKTVSTYRSRILEKMSMKSNADITTYALRNNLVQ"
     gene            complement(2626..3282)
                     /locus_tag="VEW08_16700"
     CDS             complement(2626..3282)
                     /locus_tag="VEW08_16700"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HYJ42432.1"
                     /translation="MLAKVSRQTYPFALPDLPYAYDALEPHIDAATLRIHHREHHATY
                     VKKLNEALSKEPELQNLTLAQLLRDTAKLPEAIRTAVKNNGGGHLNHDLFWKSLAPAP
                     SPAPRGALGQALEQDFGTVDSFRKKFSDAAAKHFASGWVALSLDHASRKLAIVDLKDH
                     EVLQGSDATAILILDVWEHAYYLKYQNRRPEFIEAFWNVIDWSRAAERFEHGAASSGV
                     "
     gene            3492..4070
                     /locus_tag="VEW08_16705"
     CDS             3492..4070
                     /locus_tag="VEW08_16705"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HYJ42433.1"
                     /translation="MNLKIAAALAALSVATVPAALADENAGLYLGGGVGSFNVGIDNP
                     DQVNDAVGAWDEDDNAYKLFAGYRLNKFLAFELDYINLGEPSGAVVPGFNVDLAVDGF
                     APFVVGTIPLGNWFEVYGRLGYYFYDANISVEDEINGNAEFDEESEDLVYGAGIGANI
                     GERFNARFEYERYDLEGTDDADALWLTAAWKF"
     gene            complement(4135..4509)
                     /locus_tag="VEW08_16710"
     CDS             complement(4135..4509)
                     /locus_tag="VEW08_16710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ42434.1"
                     /translation="MPPLRVLIAEDNEDLRSLLVTLVSAEPDLKCVGTAADVESVIAS
                     TAENKADVLVLDLELQGRSSIDLLRSVRRNGSKITIVVFSGHSHPEMIRHTLAAGADA
                     YIPKSGDYELLLDAIRDKRVTT"
     gene            complement(4581..4796)
                     /locus_tag="VEW08_16715"
     CDS             complement(4581..4796)
                     /locus_tag="VEW08_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42435.1"
                     /translation="MANSLTACLGRFSPGNHLAWHEAPDGSPRHKLDRLRELRAVIDT
                     LRCEQEVQDPLLNWILERERVENHRRD"
     gene            4960..5220
                     /locus_tag="VEW08_16720"
     CDS             4960..5220
                     /locus_tag="VEW08_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42436.1"
                     /translation="MPKQKIKGEGDYAATRRYRKRTDEYLNNNDVEKAAVRAAPTSAR
                     EAEDMQAAEAAGKRRAKGEDPALRRRSAPRVEPSTRGKTSRH"
     gene            complement(5250..6104)
                     /locus_tag="VEW08_16725"
     CDS             complement(5250..6104)
                     /locus_tag="VEW08_16725"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ42437.1"
                     /translation="MFDGFTLTTVETDEVTIRLRHGGSGPPLLLLHGFPQTHAMWSKV
                     APRLAENYTVVCPDLRGYGGSSKPASSANHETYSKRAMARDQLEVMKKLGFERFFVAG
                     HDRGGRCAYRLALDSPASVSKLAVLDIVPTGDTLRRADWRFGMGYWHWFFLAQAHPLP
                     ERMISADPVAFLFRHGQASSDFEAYEDFLQNARDPAAIHAMCEDYRAAVSVDFQQDQA
                     DRGTRKIECPTLVLWGNRGVLPKWYDPVGIWKDWASDVRGEEVESGHMVAEEAPDATY
                     QALRRFFG"
     gene            complement(6124..8505)
                     /gene="pbpC"
                     /locus_tag="VEW08_16730"
     CDS             complement(6124..8505)
                     /gene="pbpC"
                     /locus_tag="VEW08_16730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HYJ42438.1"
                     /translation="MGRSDFTAALSRARPLRHRRVLAAVVLCACAAAIGFWAMLPAAL
                     FDAPESYVLEARDGTLLSARIASDGQWRFPPQATVPRKFRRALLVFEDKRFEQHSGVD
                     GLAIARALRLNLEAGRVVSGGSTITMQLARLSRDGKKRSLANKAAEALLALRIEASFG
                     KDEILALYAGHAPFGGNVVGLEAAAWRYFGRDPETLSWAEVATLAVLPNNPALVHLAR
                     NRERLREKRDFLLRRLHEAGDLTALDLELAVSEPLTAEPHDLPDLAPHLLDTLRAQNP
                     ARHRIVTTLDARLQRNATQLTREHSTLLARQNVYNAAALIVDNTTFEVLAYVGNSSEA
                     NDPLRGHAVDVIRRPRSTGSILKPLLYAAMLEDGALTPHMLLPDVPTHYDGFAPENFD
                     RQYRGAVPADEALAHSLNVPAVRMLKTYGVARFADLLRAAGMSTLTRPSDDYGLTLVL
                     GGAEGNLWDVSALYASLAGLARAGEADPAPHFRELTVLRGAPARTRSGVAIGTGAAWL
                     TLDTLLEVPRPGEEGHWRNFASSRSIAWKTGTSWGLRDGWAIGNSSRHTVAVWVGNAS
                     GEGRPGLTGSAMAAPLMFSLFNSLPASPWFEMPTYALRRIDTCENDGYLATGACAAAR
                     TWVPANSHFDLLSPHNLRVNLGADGKQRVDSDCESPGRMMQANWFVLPPAQEFYFRRV
                     HAEYRPLPALRADCSATRPGGRPALALLYPDPNSMVLIPEELDGQRGRTVFEAISRRR
                     EATVFWHLDGQYLGETHTFHQQSLDIEPGEHILTVVDDEGERVARRFQVIATR"
     gene            complement(8475..>10323)
                     /locus_tag="VEW08_16735"
     CDS             complement(8475..>10323)
                     /locus_tag="VEW08_16735"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42439.1"
                     /translation="KLEVQTDARFSAVGSAAQQIAFTRPEEKLGFLTLRSGSALGKGK
                     IHVVATSGRYRAESDVWLEVRAPNVTSSRYQRATLAPGQSWTGDLAAFGLPGTQVANL
                     EVSALPPINLDGRLEYLIRYPYGCLEQTTSSVFPQLYLPALIRLDPNRRLEVENNIRA
                     GLARLRSLQHPNGGFAYWPGMWNTDGNHDWRNNWGTTYAGHFFLEAEKSGYSLPGDMK
                     SSWLRYQKNAAQQWNPRQNEPGRYAPDLVHAERYAQAYRLYTLALAGAPEIGSMNRMR
                     EVDSLSLAERWLLASTYKLAGKPEVAKALVDKDRVQAFVFADANPYTFGSLMRDRAIV
                     LMGMTLLGRDAETGPLLEDVAAQLSDGSWYSTQSVAFALVAVAQNTGTKPFTGFSFDY
                     AAGAARKQAVKGDAPVANIKLPPPPPTGTPLTLTNTSERKLYATVAVRAIPRSGEEDA
                     SANGINLAVSYSDADGKPLDVHRVAQGSDLIAQLTVRNVSKRQLDNLALSQLVPAGWE
                     IRNDRLEGVDTQGERSTEKQPLGKFWWVPAEWRNQALRTAEYVDIRDDRVQRFFSLGP
                     GESIFFETRLNAAYLGKFYLPGAGVEAMYEATQHARVKGQWVEVISPQR"
BASE COUNT         1825 a         3201 c         3408 g         1889 t
ORIGIN      
        1 ggtgggtatg tcgctgctca aacatcgatc gctgtggctc gcggcggtca gcttgctcct
       61 caacctgttc cggcgccgcg cgcacaagga gaagtgaaat catgaagacc aatctcaagc
      121 cgcaatccgc acctgaaggc aacgccttca tttccgacct gagtgcgctg cgctcgcgcg
      181 cgcgcgaaca gatgatgaag ggcgcggtca cgcccagctt tcccgaggcg gaccgcaagg
      241 ccatcgtcga actgctgaac acggctctcg ccacggaaat cgtctgcgtg ctgcgttaca
      301 aacggcacta ctactcgacc catggcattc gggcgaagtt cgtctcggcc gagttcctcg
      361 agcacgccaa agaagaacag gcgcatgccg accagatcgc cgagcgcatc gtgcagctcg
      421 gcgaagatcc cgatctcaat cccgctactc tgctgtctcg atcccattcg gaatacgatg
      481 aagccacgtc gctgcgccag atgctggagg cagacctctt cgccgaacgc atcgccatcg
      541 agagttaccg tgaaatgatc aatttcatcg ggccacgaga tcccacttcg cgccgcatgc
      601 tcgagtccat cctcgcccag gaagaagaac acgccgagga tctcgtcgat ctgctcgagg
      661 acttgccgaa ggattgacat gcatgatgcc gacgaacaac ccgggcggga gctcgaggcg
      721 ctcgttgctg cgcgcaccga agaactgtcc actctgtcta cccacctgct gcggcggacg
      781 gagtcggagc ggtcggcgct cgccaaggaa ctgcacgacg aacttggtgg actgatcacc
      841 gccgcgaaaa tggacatgtc gtggctgtct gcgcacctgc gcaactccct ggacgcggag
      901 agccgcgaaa agctggactc ggtgatgcag atgctcaacc aggcgatgat gctcaagcgt
      961 cgcgtggtcg aagacctgcg gccatccctg ctcgatcact ttggcctggc ggtggcgttg
     1021 cgcagtcatt tcgatgaaca ttgtgcgcgc gccggcatcg agtgcgtgtc gacgctgccc
     1081 gacgatggcc tcgagctcga cgcggtcacg caactcagcc tctttcgcgt cgcccaggag
     1141 gccctggtga acatcatctc gcgcggcggg gccaaacatg tcgaggtggt catcgaggcc
     1201 gagggcgagg gctacgtcat ggtcatcggc gatgacggcg cgcctgcgga taccaacctt
     1261 gcgcgctcca tgccgagcgc gcgtcaccgt atgcagtggg cgggcggcag tgtcgccgtc
     1321 gaggcacgcg cggaagggaa tcagatcagg gttttcgtcc cgcgatctgc tgcaggatcg
     1381 gcatgagctg atcgatttcg cgcatcttgt cgaggaagtg gtcggcgccg gccgccatgc
     1441 agcgctcacg caccgtcggc aggtcgtagt tggtcagcac gatgatccac acgtgatccc
     1501 cgttaccttt gtcggggttt tgcgcccgcg cggagcggat caccccgatg ccgctgcccg
     1561 gacgcagctc cacgtcgacc acgatggcgt cgtagggcac ggtttcgagt ttctgcacgg
     1621 cctcggcttc ggctgaggcc agccccgtca ccttgaatgc ggcgtactgc gacagcatgc
     1681 tttccagccg gctgcgcagc aggggggaat cttcgaccag caggatacgc agcggcttgc
     1741 cgggcgcgcc gttggcgcgg accgtgtcgc tcgaattctg gcgtttcacg tccgccgttg
     1801 ttcccggagc tgattgcaat gaagggggca tcgcctcgtc cgtggcgctt gcgccaaagt
     1861 gaatcggtcg tctatctact ggacgaggtt attccgcagc gcataggtgg tgatgtcagc
     1921 attactcttc atcgacatct tctcgaggat acggctgcgg taggtgctca ccgtcttgac
     1981 actgaggaat agccgattcg cgatctcgga caccgactcg ccctgcgcca gtttgcagaa
     2041 aatctggaat tcgcgttcgg acaggatgct gtgcagcggg gcattcgggt caccgcgtac
     2101 gccggcgacc agcaggtctg ccagctcggg tccgacataa cgtccgccgc gggcgacggt
     2161 gcgaatggcg cggaacagtt cggattcctc cgtgcccttg ccgacgaatc cactggctcc
     2221 accacgcagc acgttgagtc cgtattgcgc ttccgggaac gccgagagga tgagcacgcc
     2281 gacggattcg taatgagcct tggcgtgttt gagcacgtcc aggccggaaa cgtccggcag
     2341 ggacaggtcg agcagcagca catcgatggt gtgcgcccgg agctgttcca tggcctctcg
     2401 gccagaactc gcttcggtca cgaccgccag atccggctga ccgttaatca gggttttaag
     2461 gccggcgcga acaacctggt ggtcgtccac aatcatgact ctggtgcgat tcatgggccg
     2521 ggaagtctcc acaacttgtt gagttatttc gtgcatggcc ggagtctaca ttcgtgaggt
     2581 tcagcgcctg tccggcagac gggcggtacc tcagtcagaa gtatcctaca cgcccgacga
     2641 agctgcgccg tgttcgaatc tctcggcagc cctcgaccag tcgatgacgt tccagaatgc
     2701 ttcgatgaat tccggccggc gattctgata tttcagatag taagcgtgtt cccagacgtc
     2761 gaggatgagg atcgcggtcg catcgctgcc ttgcagcact tcgtgatcct tgagatccac
     2821 gatcgcaagt ttccgcgacg catgatcgag cgacagtgcc acccagccgc tcgcgaagtg
     2881 cttcgcggcc gcgtcgctga atttcttgcg gaatgaatcc acggtgccga agtcctgctc
     2941 cagtgcctgc cccagagctc cgcgcggcgc gggtgaaggc gcgggcgcaa gcgatttcca
     3001 gaacagatca tgattgagat ggccgccacc gttgttcttc acggcggtgc gaatcgcctc
     3061 gggtagtttg gcggtgtcgc gcagcagttg tgcgagcgtg aggttctgca gttccggttc
     3121 cttcgacagc gcttcgttga gcttcttcac gtacgtggcg tggtgttcgc gatgatgaat
     3181 gcgcaatgtc gcggcgtcga tgtgcggctc gagcgcgtcg taggcgtagg gcaggtcggg
     3241 cagcgcgaac ggataggtct gacgggaaac cttggcgagc acgggcgtct cctcgatgga
     3301 aagcgactgc ggcgagcatg cgcgcgtgac gcggcgacgc gaatcatcgc aaaaccaggg
     3361 gcgaggtaag actcctccga tacccggcca ccgacggaga gaaccgacag gcatgggagg
     3421 tcatgcctga gtgtggtgca tttgcgcaac gcctaacttc accgccgttc aaatcaaccc
     3481 aaggatcaat catgaatctc aaaatcgccg cggcacttgc cgcattgtca gtagcgacgg
     3541 tgcccgctgc ccttgccgat gaaaatgccg gtttgtatct cggtggtggc gttggctcgt
     3601 tcaacgtcgg tatcgacaac ccggaccagg tcaacgacgc ggtcggtgca tgggatgagg
     3661 atgacaacgc gtacaagctc ttcgcgggct atcgcctcaa caagttcctg gcgttcgagc
     3721 tcgactacat caacctcggt gagccatcgg gcgccgtggt gccgggattc aacgtggatc
     3781 tggcggtaga tggcttcgcg ccgttcgtcg tcggcacgat tccgctcggc aactggttcg
     3841 aagtctacgg gcgtctcggc tattacttct acgacgccaa catcagcgtc gaagacgaga
     3901 tcaacggcaa cgccgaattc gacgaagagt ccgaggacct ggtctacggc gccggcatcg
     3961 gcgcgaacat cggtgagcga ttcaacgcgc gcttcgagta cgaaaggtac gatctcgaag
     4021 ggaccgatga tgccgatgcg ttgtggctga ccgccgcctg gaagttctga tagaaaattg
     4081 ccgtcttttt gcggcaacaa acaccgggcc gccctcgcag gaggcggccc ggtttcatgt
     4141 ggtgacgcgt ttgtcgcgta ttgcgtcaag cagcaattcg tagtcgccgc tcttcggtat
     4201 ataagcgtca gccccggcgg ccagggtgtg acgaatcatc tcggggtgcg agtgcccgga
     4261 gaaaaccacg atcgtgatct tgctgccatt gcggcgcacg ctgcgcagca ggtcgatgct
     4321 cgagcggccc tgcagctcca ggtcgagcac gagaacgtcg gccttgttct ccgcggtact
     4381 ggcgatcacc gattccacat cggccgccgt gcccacgcat ttcaggtcgg gctcggcgga
     4441 cacgagcgtg accaacaagg agcgcaggtc ctcgttgtct tcggcgatca gaactcggag
     4501 cggtggcacg gtcatgacgc gtgaagaata cctgcggatg ccggcggggc gcgcattgtc
     4561 gcagcccgac gaccccgtgt tcaatcgcgt cggtggtttt cgacccgttc ccgttcgagg
     4621 atccagttca gcagggggtc ctggacctct tgctcgcagc gcagcgtgtc gatgacagcg
     4681 cgtaactcgc gcaggcggtc gagtttgtgc cgtggcgagc cgtccggtgc ctcgtgccag
     4741 gcgaggtggt tgccgggcga aaatcgcccc aggcaggcgg tcaatgaatt tgccatttcc
     4801 agaaggctcc gcatcgttcc ctgatggctt gcagtctcgc aaacctgcca atcgagggaa
     4861 tctgcgcgat tgctgattgt ggcgtaggac gttaccgcag tgcgccatcg acacacgaaa
     4921 ttccgggtct aatgagctgc cccacacgga ggaattccca tgccgaagca gaagatcaag
     4981 ggcgaaggcg actacgcagc cacgcggcgc taccgcaagc gcaccgacga gtacctcaac
     5041 aacaacgacg tggagaaggc cgcggttcgc gccgcgccaa cttcggcgcg tgaggccgaa
     5101 gacatgcaag ccgccgaggc ggccggcaag cgtcgcgcga aaggtgagga cccggcattg
     5161 cggcggcggt cggcgccacg cgtcgagccg tcgactcgcg gcaagacctc gcggcactag
     5221 ttagtgcggg gcgaccggcc gaagccttct cagccgaaaa acctgcgcag cgcctggtag
     5281 gtggcgtcgg gagcttcctc agccaccatg tggccgcttt cgacctcttc accgcgaacg
     5341 tcgctggccc agtctttcca gatgccgacg gggtcatacc atttcggtaa cacaccgcgg
     5401 ttgccccaga gcaccagcgt ggggcactcg atcttgcgtg tgccgcggtc cgcctggtcc
     5461 tgctggaagt ccacgctcac cgcggcgcga tagtcttcgc acatcgcgtg gattgccgcc
     5521 gggtcgcgcg cgttctgaag gaaatcttcg taggcctcga aatccgaaga ggcctgtccg
     5581 tgacggaaca gaaacgcgac ggggtccgcg ctgatcatgc gttccgggag ggggtgcgcc
     5641 tgcgcgagaa agaaccagtg ccaatagccc atgccgaagc gccagtcggc gcggcgcagg
     5701 gtgtcgcccg tcggcacgat gtcgagcacc gcgagtttgc tcacggatgc cggcgagtcg
     5761 agggcgaggc ggtaggcaca gcgtccgcca cgatcgtggc cggccacgaa gaatctctcg
     5821 aatccgagct tcttcatgac ctcgagctga tcgcgcgcca tggcccgctt tgagtaagtt
     5881 tcgtggttgg cgctggaggc gggcttgctg ctgccaccgt agccgcgcag gtcgggacaa
     5941 acgacggtgt agttctcggc gaggcgtgga gccaccttcg accacatggc gtgagtctgg
     6001 ggaaatccgt gcaggagcag gagaggtggt cccgaaccgc cgtggcgcag gcggatggtg
     6061 acttcgtcgg tctcgactgt tgttagcgta aatccatcga acacgacacc accttaaaaa
     6121 gccttaccgc gtggcgatga cctggaagcg ccgggcgacg cgttcccctt cgtcatccac
     6181 cacggtcagt atgtgctcgc cgggttcgat gtccaacgac tgctggtgaa aagtgtgcgt
     6241 ttccccaagg tattggccat cgagatgcca aaagaccgtg gcctcgcgac ggcgggaaat
     6301 ggcctcgaag acggtgcgtc cacgctgtcc atcgagctcc tcagggatca gcaccattga
     6361 attcggatcg ggatatagca gcgcgagcgc gggacggcca ccggggcgcg tggcgctgca
     6421 gtccgcgcgc aacgccggca acggccgata ttcggcgtgc actcgacgaa agtagaactc
     6481 ctgcgcgggc ggcaaaacga accagttcgc ctgcatcatc cggccggggg attcgcaatc
     6541 gctgtcgaca cgttgtttgc catcggcgcc gagattgacg cgcaggttgt gagggctcaa
     6601 caaatcaaaa tgactgttgg ccgggaccca ggtgcgggcg gcagcgcagg cgccggtcgc
     6661 aagatacccg tcgttctcac aggtatcgat acgccgcagc gcgtaagtgg gcatctcgaa
     6721 ccacgggctc gcgggcagcg agttgaacag cgaaaacatg agtggcgcgg ccatcgccga
     6781 gcccgtgagt cccggacggc cttcaccgct ggcgttgccc acccacacgg ctaccgtgtg
     6841 acggctggag ttgccgatcg cccagccgtc gcgcaggccc cagctcgttc cggtcttcca
     6901 ggcaatactg cggctggaag cgaagttgcg ccagtggcct tcctctccgg gccgcggcac
     6961 ctctaacaag gtgtcgagcg tgagccaggc ggcgccggtt ccgatggcga cgccgcttcg
     7021 ggtgcgtgcg ggcgcgccgc gcaggaccgt gagttcgcga aagtgcggcg ccgggtccgc
     7081 ctcgccggcg cgtgcgagtc cggccaggct ggcgtacagg gctgacacat cccagagatt
     7141 cccttccgcg ccgccaagaa cgagcgtgag cccgtagtca tcgctcgggc gggtgagcgt
     7201 gctcatgccc gcggcccgca gaaggtcggc gaaacgcgcc acgccgtagg tcttgagcat
     7261 gcggaccgcg ggcacattga gcgaatgcgc gagcgcctca tcggccggca ccgcgccgcg
     7321 atactggcga tcgaagttct cgggcgcgaa cccgtcgtag tgagtgggaa cgtcgggcag
     7381 gagcatgtgc ggggtgaggg cgccatcctc gagcatggcg gcgtacagca gcggcttgag
     7441 gatgctgccg gtactgcgcg gccggcggat cacatccact gcgtgaccgc gcaacgggtc
     7501 gttcgcctcg ctggaattgc cgacataggc cagcacttcg aaggtcgtgt tgtcgacgat
     7561 cagcgccgcg gcgttgtaca cgttctggcg cgcgagcagg gtcgagtgct cacgcgtgag
     7621 ttgagtggcg ttgcgttgca gccgcgcgtc gagcgtggtc acgatgcggt ggcgggcggg
     7681 attctgcgcg cgcagcgtgt cgagcaggtg cggtgcgagg tcgggcaggt cgtgcggctc
     7741 ggccgtgagc ggctcgctca ccgccagttc gaggtcgagc gcggtcaggt cgccggcctc
     7801 gtgcagccgc cgcagcagaa agtcacgctt ttcccgcagg cgctcgcggt tgcgcgccag
     7861 gtgcacgagc gcggggttgt taggcagaac cgccagcgtt gccacctcgg cccacgaaag
     7921 cgtttcggga tcgcggccga aataacgcca ggcggccgct tcgaggccca cgacattgcc
     7981 gccaaacggc gcatgtccgg cgtacagcgc gaggatctcg tctttgccga aggacgcctc
     8041 gatgcgtaac gcgagcagtg cttccgcggc cttgttggcg agactccgtt ttttgccgtc
     8101 gcgcgacagg cgcgcgagct gcatggtgat cgtgctgccg ccgctcacca cgcggccggc
     8161 ctcgaggttc aggcgcaggg cgcgggcgat tgccaggccg tcgacgccgg agtgctgctc
     8221 gaagcgtttg tcttcgaata ccagcagcgc ccggcggaat ttccgtggca cggtggcctg
     8281 cggtggaaaa cgccactgcc cgtcgctggc aatgcgggca ctcagcaaag tgccgtcgcg
     8341 cgcttcgagc acgtaggact cgggcgcgtc gaacagcgcg gcagggagca tcgcccagaa
     8401 gccgatggcc gccgcgcatg cgcacagcac caccgccgcg agtacacggc ggtggcggag
     8461 cgggcgcgcc cggctcaacg ctgcggtgaa atcacttcga cccattggcc cttgaccctc
     8521 gcgtgttgcg tggcttcgta catcgcctcg acaccggcac ccggtagata gaatttgccg
     8581 agataggcgg cattgagccg cgtctcgaag aagatcgact cgccgggtcc caggctgaag
     8641 aagcgctgca cacggtcgtc gcggatatcc acgtattccg cggtgcgcag cgcctggttg
     8701 cgccattccg ccggaaccca ccagaacttc ccgagcggct gcttctccgt gctgcgctca
     8761 ccctgcgtgt cgacgccctc gaggcggtca ttgcgaatct cccagcccgc gggcacgagc
     8821 tgcgagagcg cgagattgtc gagctggcgt ttgctgacgt tcctgaccgt gagttgcgcg
     8881 atgaggtcgc tgccctgggc gacgcggtgc acgtcgagcg gcttgccgtc ggcgtcgcta
     8941 taactcacgg ccaggttgat gccgttggcg gaggcgtctt cctcaccgct gcgcgggatc
     9001 gcacgcaccg caaccgtggc gtacagcttg cgttccgagg tgttggtgag cgtcaatgga
     9061 gtgccagtcg gcggcggcgg cggtagtttg atgttggcga ctggcgcatc acccttgacg
     9121 gcctgcttgc gtgcggcacc cgccgcgtag tcgaagctga agccggtgaa tggcttcgtc
     9181 ccggtgttct gcgccacggc caccagcgcg aaagccaccg actgcgtgct gtaccagctg
     9241 ccgtccgaga gctgcgcggc cacgtcctcg agcagcggcc cggtctcggc gtcgcggccg
     9301 aggagcgtca ttcccatgag cacgatggcc cggtcgcgca tcagcgatcc aaaggtgtag
     9361 ggattggcat cggcgaacac gaaggcctgc acgcgatcct tgtcgaccag cgcttttgct
     9421 acctcgggct tgccggcgag cttgtaggtc gaggcgagca accagcgttc tgcgagcgac
     9481 agcgaatcga cttcgcgcat ccggttcatg gagccgatct cgggcgcgcc ggcgagcgcc
     9541 agtgtgtaca ggcgatacgc ctgcgcgtaa cgctcggcat gcacgaggtc gggcgcgtaa
     9601 cggccgggtt cgttctgtcg cgggttccac tgctgcgcgg cattcttctg atagcgcagc
     9661 cacgaagact tcatgtcgcc gggcagcgag tatcccgatt tttcggcttc caggaagaag
     9721 tggccggcgt acgtcgttcc ccagttgttg cgccagtcgt gattgccgtc cgtgttccac
     9781 atgcccggcc agtaggcgaa gccgccattc gggtgctgca ggctgcgcag gcgtgcgagt
     9841 cccgcgcgga tattgttctc cacctcgagc cggcggttcg ggtcgagcct gatcagcgcc
     9901 ggtagataga gctgcgggaa cacgctggaa gtcgtctgct ccaggcaccc gtacggatac
     9961 cggatgaggt attccagtct tccgtcgaga ttgattggcg gcagcgccga cacttccaga
    10021 tttgccacct gggttcccgg cagtccgaaa gccgcgaggt ctcccgtcca ggactgcccg
    10081 ggcgccagcg tcgcgcgctg atagcgcgag gaggttacat tcggcgcgcg cacttcgagc
    10141 cacacgtcac tttccgcgcg atacctgccg ctggtggcga ccacgtggat cttgcctttg
    10201 cccagcgccg agccagagcg aagcgtgagg aacccgagct tttcttcggg acgcgtgaaa
    10261 gcgatctgct gggcggcgct gccgacggcg ctgaaacgcg cgtcggtctg cacctcgagt
    10321 ttc
//
LOCUS       DASQJS010000424         4890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3426221, whole genome shotgun sequence.
ACCESSION   DASQJS010000424 DASQJS010000000
VERSION     DASQJS010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3426221"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /gene="thrC"
                     /locus_tag="VEW08_16740"
     CDS             <1..1189
                     /gene="thrC"
                     /locus_tag="VEW08_16740"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HYJ42440.1"
                     /translation="VSSLPEVAEVMLRPFVAGDPIAAEIGNIVRDAFNFPAPLVPVAD
                     DGKLSVLELFHGPTAAFKDFGARFLAASLARIRQSAVSAKPLNILVATSGDTGGAVAA
                     AFHRRPGVVVSVLFPKGLVSPTQERQLTCWGDNVLSYRVNGSFDDCQRLVKQAFVDEE
                     LRKRFDLSSANSINLGRLLPQAVYYAATSLAVHREHGVVPNFLIPSGNLGNSVACVWA
                     RKLGLPIGRIVLAHNANRAVPDFLESGEWRPRASVPTLASAMDVGSPSNMERLRALFP
                     DIGGVRGAVRAESVSDDEIRARIATDFHRFKGQYGKPWCPHTATAAEVHARMPISERS
                     NARWVLVSTAHPAKFREIVEPLIGQRIEIPETLAKLFSRPVSCADLEPSLAALTGALG
                     NKI"
     gene            1186..1800
                     /locus_tag="VEW08_16745"
     CDS             1186..1800
                     /locus_tag="VEW08_16745"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HYJ42441.1"
                     /translation="MNPPFGLTQVEFILVVVGAIVVALLGLLGSRAWVVRRRDARRVA
                     RVTAGAADFLRNVLVPDGNGGDYHLDFVLLTSRGIVVIDMRDITGNVFGGDQMTAWTL
                     MSGPSRTTFVNPQAGLYDRIASVKAIATDTPIEGRIVFTKHAKFPKGLPRFTVMLESV
                     ASEFPKLGAAELEIAVSKYRPGWARLKEAAKPSPHFRNNPIAVA"
     gene            complement(1797..2888)
                     /locus_tag="VEW08_16750"
     CDS             complement(1797..2888)
                     /locus_tag="VEW08_16750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ42442.1"
                     /translation="MQNAARSTAIHSPSINEDVLNALLGRAITDFGATSLAALVVIGD
                     RLGLYRTLATSGALTASELASKSGTHERYVREWLNAHAASGYVNYLADSGRYQLSPEQ
                     AMLFAQEESPAFIVGGFQTALAAGRIVDRLTDAFRSGDGIGWHEHHPDMFPGCARFYK
                     VGYLNHLVQEWIPALDGVEAKLKAGIRVADVGCGVGYSTLIMARAFPNSHFTGFDYHV
                     ESIEAAVGHAREAGVTNVSFEIGKADNYRGHSYEFVTVFDALHDMGDPLAASRHVLKT
                     LAPNGTWMIVEPYAGDRIEENLNPLGRAYYAASTLLCTPCSLSQEGRMGLGAQAGEAR
                     LRSVVTGAGFKRFRRAMHSAVNLILEARP"
     gene            3033..4049
                     /locus_tag="VEW08_16755"
     CDS             3033..4049
                     /locus_tag="VEW08_16755"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYJ42443.1"
                     /translation="MDALSALLRAVKLSGALFYNSRCSAPWCLNAPPSSAFLPYVTPG
                     ATHIIEFHYISEGTAYVRVGVETTPLVAGDVVMIPHGDAHVMGAGVGGVVLDGNKAMP
                     ALIAGELFLSQFGGGGENTGLVCGYLACDGALIKPVLAGLPRALRVNIRSDPSGEWLE
                     KTLRHAVEQAAAGAPGSDVILAHLAEVLFAEVLRRYLLSLPEGRTGWLAGAGDPAVGR
                     ALAVLHRRPADDWTLDTLAKEAGVSRSVLTEKFTRFLGQSPMSYLADWRLELGAEALR
                     STSRSVQRIANDVGYESEAAFNRAFKRRFDAPPARYRRAAREVGPKSDTRPAPAMRRQ
                     AGGA"
     gene            4042..4809
                     /locus_tag="VEW08_16760"
     CDS             4042..4809
                     /locus_tag="VEW08_16760"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HYJ42444.1"
                     /translation="MLESKPQDNTDAPRCEACGAVLVGRYCHECGQQRLDHRLTTRDL
                     AADFARRVLRFDKSFVETLWRAIRMPGRLAHDYLAGRRRGILDPIVYFVGTVFIQFLL
                     AVLAHMLAYAFGGDVIFDSLGTVGGMVVLKICVIFFAAGVWRLLTRGDEYSTAEIAVF
                     AIYCFGTLGLLWAVLPLTELLLRVPLSAHRGVTVLVVLAIAAVYLTLGIREFARTSYV
                     LAAARAVFTLGLAYGLMAATLGIGRSIDFMAPLLGAP"
BASE COUNT          781 a         1608 c         1596 g          905 t
ORIGIN      
        1 cgtcagcagc ctgccggaag tcgccgaggt catgctgcgc ccgttcgtcg cgggcgatcc
       61 gattgccgcg gaaatcggca acatcgtgcg cgatgcattc aattttccgg cgccgctggt
      121 gccggtggca gacgatggca agctgtcggt gctcgagctt ttccacgggc ccaccgcggc
      181 cttcaaggat ttcggcgcgc ggtttctcgc ggcctcgctc gcgcgcatcc gccagagtgc
      241 ggtcagcgcc aagccgctca acattctggt cgcgacctcg ggtgacaccg gcggtgcggt
      301 ggcggcggcg tttcatcgcc ggcccggcgt cgtggtttcc gtgctgttcc ccaagggtct
      361 cgtttcgcct acccaggagc gccagctcac ctgctggggc gacaacgtgc tgtcgtaccg
      421 cgtcaacggc agtttcgacg actgccagcg gctggtgaag caggccttcg tcgatgaaga
      481 gctcaggaaa cgcttcgatc tgtcgagcgc caacagcatc aatctcggac gcctgctgcc
      541 ccaggccgtt tattacgccg ccaccagcct cgcggtgcat cgcgaacacg gcgtggtgcc
      601 taacttcctg atcccgagcg gcaatctcgg caactccgtt gcctgcgtat gggcacgcaa
      661 gctgggcttg cccatcggcc gcatcgtcct ggcgcacaac gccaatcgcg ccgtgccgga
      721 tttcctggaa agcggcgagt ggcggccccg cgccagcgtt ccgacactgg cgtcggccat
      781 ggacgtgggc tcgcctagca acatggaaag gctgcgtgca ctgtttccgg acatcggagg
      841 tgtacgcggt gccgtgcgtg cggaaagcgt cagcgacgat gaaatccggg cacgaatcgc
      901 caccgatttt catcgattca agggtcagta cgggaagccc tggtgcccgc ataccgccac
      961 cgcggcggaa gtccatgcgc ggatgcccat ttccgagcgt tcgaatgcgc gctgggtgct
     1021 ggtctcgacc gcgcatccgg caaaatttcg cgaaatcgtg gagccgctca taggccagcg
     1081 aatcgaaatt ccggaaaccc ttgctaaact cttctcgcgt cccgtgtcct gcgccgatct
     1141 cgagccctcg ctcgccgcgc tcacgggtgc gctggggaac aagatttgaa tccgcctttc
     1201 ggactcacac aggtcgagtt tattctggtc gtcgtcggcg cgatcgtcgt ggcgctgctc
     1261 ggcctgctgg gcagtcgcgc ctgggtggtt cgccgtcgcg acgcgcgccg cgtcgcacgt
     1321 gtcaccgccg gcgcggcgga ctttctgcgc aatgtgctcg tccccgatgg caacggcggc
     1381 gactaccacc tcgatttcgt gctgctgacc agccgcggaa tcgtcgtcat cgacatgcgt
     1441 gacatcaccg gcaatgtgtt cggcggcgac cagatgaccg cctggacgct catgagcgga
     1501 ccgagccgca ctaccttcgt gaatccgcag gccggcctct acgaccgcat cgccagcgtg
     1561 aaggccatcg ccaccgatac gccgatcgag ggccgaatcg tcttcaccaa gcacgccaag
     1621 tttccgaaag gcctgccgcg tttcacggtg atgctcgaat ccgtcgcctc ggaatttccc
     1681 aagctgggcg ccgccgaact ggaaattgcc gtttcgaagt accgtccagg ctgggcccgg
     1741 ctcaaggaag ccgccaagcc cagcccgcac ttcaggaaca accccatcgc ggtggcttaa
     1801 ggcctcgctt cgaggatcag gttcactgcc gagtgcattg cccggcggaa tcgcttgaat
     1861 cccgcaccgg tcaccaccga tcgcagtcgc gcttcgccag cctgcgcacc caaacccatt
     1921 cggccttcct gcgacagcga gcagggcgta cacaacagcg tggatgcggc gtagtacgcg
     1981 cgcccgaggg ggttgaggtt ctcctcgatg cgatcaccgg cgtatggctc gacgatcatc
     2041 caggtgccat tcggcgccag cgtcttcaac acgtgacgcg aagcagcgag cgggtcgccc
     2101 atgtcgtgca gcgcatcgaa caccgtgacg aactcgtatg agtggccacg gtagttatcc
     2161 gccttgccga tctcgaagct cacattggta actccggctt cgcgcgcatg cccgacggcc
     2221 gcttcaatcg attcgacgtg gtagtcgaag ccggtgaagt gcgagttggg aaacgccctg
     2281 gccatgatca gcgtcgagta acccacaccg cagcccacat cggcgacccg gatgccggcc
     2341 ttgagtttgg cttcgacgcc atcgagcgcg gggatccatt cctggaccag gtgattgaga
     2401 tatcccactt tgtagaaccg ggcgcagccg gggaacatgt cggggtgatg ttcgtgccag
     2461 ccgatgccgt ctccggagcg gaaggcgtcc gtgaggcgat ccacgatgcg gccagcggcc
     2521 agcgcggtct gaaagccgcc gacgatgaac gccggactct cctcctgggc gaataacatc
     2581 gcctgttcgg ggctcaactg gtagcggccg ctgtcggcca ggtaattcac gtagccggac
     2641 gcggcatggg cattgagcca ttcgcgtacg tagcgctcgt gcgtgccgct tttgctggca
     2701 agctcggaag cggtgagcgc gccactcgtc gcgagggtgc ggtacagccc gaggcgatcg
     2761 ccgatgacca ccagcgcggc gagcgaagtg gcgccgaagt cggtgatggc gcggcccaga
     2821 agggcgttga gcacgtcttc gttgatggaa ggcgagtgga tggcggttga tcgagctgcg
     2881 ttctgcatga tgtgtctcca ggttgcgagg gtgcgcatac tgaaggcacc atgtcgcggg
     2941 agactttgct gcccgaccgg acgtcttgat cgcgcggccg cgggccgcag catgtttgcc
     3001 cgatctgccg cagaatttgc tccggaacac ctatggacgc tctatccgca ttgctgcgcg
     3061 ccgtgaaact ctccggcgcc ctgttctaca actcgcgctg ctcggcgccg tggtgcctga
     3121 acgcgccgcc ttcgagtgcg ttcctgccgt acgtcacgcc cggggccacg cacatcatcg
     3181 agttccacta catcagcgag ggcacggcgt atgtgcgcgt gggcgtggag acgacgccgc
     3241 tcgtcgcggg cgacgtcgtg atgattccgc atggcgacgc gcacgtcatg ggcgcaggcg
     3301 tcggtggcgt ggtgctcgac gggaacaagg cgatgcccgc gttgatcgcc ggcgaactgt
     3361 tcctctcgca attcgggggt ggcggcgaga acaccggcct cgtgtgcgga tacctggcct
     3421 gcgacggcgc gctcatcaag cccgttctcg ccggcctgcc gcgcgcactg cgcgtcaaca
     3481 tccgctccga tccttccggc gagtggctgg agaaaacgct gcgccacgcg gtggagcagg
     3541 ccgctgccgg cgcgcccggc agcgatgtga tcctcgcgca cctggccgag gtgttgttcg
     3601 cggaagtcct gcggcgctat ctactgagcc tgccagaagg gcgcaccggc tggctcgccg
     3661 gcgcgggaga tcctgcggtc gggcgcgcgc tcgccgtgct gcaccggcgc cccgccgatg
     3721 actggacgct ggacacactg gcgaaagaag cgggcgtttc gcgctcggta ctcacggaaa
     3781 agttcacgcg tttcctcggg cagtcgccga tgagttacct cgccgattgg cgcctggagc
     3841 tcggtgcgga ggcgctgcgc tccaccagcc gcagcgtgca gcggatcgcc aatgatgtcg
     3901 gttacgaatc cgaggccgcg ttcaatcgcg cattcaagcg gcgattcgac gcgccaccgg
     3961 cgcgttatcg gcgcgccgcg cgcgaggtgg gaccaaagtc cgatacgcgc cccgcgcccg
     4021 ccatgagaag acaggcgggt ggtgcttgaa tcgaaaccgc aggacaacac cgatgccccc
     4081 cgctgcgaag cctgcggagc cgtgctggtc ggccggtact gccatgagtg cggccagcag
     4141 cgcctcgacc acaggctaac tacccgggat ctggccgcgg acttcgcgcg gcgcgtgctg
     4201 aggttcgaca agtcgttcgt cgagaccctg tggcgcgcga tccgcatgcc cggtcgcctg
     4261 gcccacgact acctggccgg ccgccggcgc gggatcctcg atcccatcgt ctatttcgtc
     4321 ggcaccgtct tcatccagtt cctgctcgcc gtactggcgc acatgctggc ctatgcgttc
     4381 ggaggcgacg tcatcttcga ctccctgggc accgtcggcg gaatggtcgt gctcaagatc
     4441 tgcgtgatct tcttcgcagc cggggtgtgg cgcctgctga cgcggggtga cgagtactcc
     4501 acggccgaaa tcgccgtctt tgccatctac tgcttcggca cattgggact gctgtgggcg
     4561 gtgctgccat tgaccgagct gctgttgcgc gtgccgctct cggcccatcg gggcgttacc
     4621 gtgctcgtcg tgctggcgat cgcagcggta tacctcacgc tcgggattcg cgaattcgcg
     4681 cggacgtcct atgtgctcgc tgccgcaagg gccgtgttca cgctgggact ggcgtatggg
     4741 ctcatggccg caacgctcgg aatcggccgc tcgatcgact tcatggcgcc gttgttaggg
     4801 gctccgtgac tccgcggaaa tcgcgaagaa gtcgcgcgcg gttcgcgtgg attcctttgc
     4861 gacctgcccg ggatcttcgc ccctcgcggc
//
LOCUS       DASQJS010000425         7427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4050734, whole genome shotgun sequence.
ACCESSION   DASQJS010000425 DASQJS010000000
VERSION     DASQJS010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7427
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4050734"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..650)
                     /locus_tag="VEW08_16765"
     CDS             complement(33..650)
                     /locus_tag="VEW08_16765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ42445.1"
                     /translation="MKDLPQTVFVVDDDDAVRDSLRMLLKSAGVHAEVCASAQDFLSS
                     YDASQPGCMVLDVRMPGMSGLEMQHELNLRGVTMPVIFITGHGDIPMAVEAMQHGAFD
                     FLQKPFRDQELLDRVQRALVRDTENRARLRHTDRIRERLASLSPREREVLDLVTQGKA
                     NKMVAGDLGVSQRTVEIHRAHVMQKMEAGSLAELVRMMMAVAPPP"
     gene            complement(647..2152)
                     /locus_tag="VEW08_16770"
     CDS             complement(647..2152)
                     /locus_tag="VEW08_16770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYJ42446.1"
                     /translation="MTAPTPEVLALLEATLDAVVIMDDRGRIEAFNASAEVMFGYRAA
                     DALGRNVSMLMTAVDRIGHDDHLRRYLAGGEARVLGRGRDVRVQHRDGSEFSVFLSVG
                     RIPNTSPPRFVGFLHDTSLRQKALATLEKERQVNRLYLDLAQVMLVATDRNGLVQLAN
                     QRAIRVLKREHAAIVDSNWLESCVAPEDRSTARAAFRSLLMSGNDEPQVCDYHVRSSD
                     GEDRFITWRGVALRDADNFATGVMLSGEDITDQRRAESEAHRALERMNSVARLATMGE
                     MAAGISHELNQPLAAIANYAQACVRFLRLPTHDLPEIAGALEQIASQALRAGEIIRRI
                     RSLVRNEDVRRETQDVNELIREVHGLLASDARVHDGKLALDLASPLPNVTVDGVQIQQ
                     VLMNLVHNAFEAQAPEHDGAPFEVRIATRCTDTGDVEVSVSDLGPGLAGEAEQKIFEP
                     FFTTKASGTGLGLAISRSIIKAHDARLGYRANQPRGACFYFVLPAHQEAPS"
     gene            complement(2204..2467)
                     /locus_tag="VEW08_16775"
     CDS             complement(2204..2467)
                     /locus_tag="VEW08_16775"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HYJ42447.1"
                     /translation="MPREKNPATPTVRKRTSRKTVTTDTGESRPTLTRFVDPERRAAL
                     IAEAAFFRAEQRGFDPGHEMEDWLAAESEVDARLMSAKAGSRA"
     gene            complement(2637..3623)
                     /locus_tag="VEW08_16780"
     CDS             complement(2637..3623)
                     /locus_tag="VEW08_16780"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYJ42448.1"
                     /translation="MRPIRRILVAVKNPTARVLPAVNKAAQIAKGLGAQLTLFHDIAT
                     PLYAEAMQGREFDLPSWQREVQTARREQLEKLAVRIRKHGIAVDVVADWDYPPFEAII
                     RKSQRLAADLLVVENHHGTGRHPARWLLAYTDWELLRLCPIPVLLVKNRRLYHRPRVL
                     AAVDPSHAFAKPSNLDRQILRAGAQLVHALRGELHALHVFHAGVPVVPAMPDGLMIDV
                     AAERDLERAARRKLERAVDGFDVKRSHCHLVEGRPNEAIPGAAKRERSAIVAMGIVSR
                     SGLRRFFIGNTAEFVMDAVTADILVVKPADFASRVPRTGRGVQILSMPILPG"
     gene            complement(3694..5091)
                     /locus_tag="VEW08_16785"
     CDS             complement(3694..5091)
                     /locus_tag="VEW08_16785"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYJ42449.1"
                     /translation="MIFSRSISWPIAAGALLALAACGSTPTRERPLKQIVPLTAPVLA
                     PGVESNTDKLFVVVTFSGGGKRAAAFSFGVLEALRDVKVTTPDGEQRSLLDEIDLISA
                     VSGGAFTAAYYALFGKEAFDSYAPRFLDHDTEEDLWHKVLKPTSWPRLAKPLVSRSDL
                     EVEYFDEELFNGATVYDIMTRSPRVIISATDLVRAQPFAFTREQLNGICVDPKSVPVA
                     RAVFASAATPIYFAPLVMQTFAGQCGYVPPESVTDAAAESDRYRRERAKRLLSYLDSE
                     HLPYLHLADGAFADNLGARAILDEVALGDTVTDALRRNGFARARRMLFIVVDARTGFD
                     ETHAQRPTPLGIKKVMDAVVSVTFNRYSSETMNLMRARVKNWESEVRNTRCNVALAIP
                     DCEKFDVDLVELSFERVKDPAERKYLDLVPTAFTISSEQTARLRQAAKLLVDESSELR
                     VFLEESAKSANKSDK"
     gene            complement(5112..5546)
                     /locus_tag="VEW08_16790"
     CDS             complement(5112..5546)
                     /locus_tag="VEW08_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ42450.1"
                     /translation="MSSTEYEISTDPARLDIEAIHAYLTRSYWSPGIPLTTVERAARN
                     SLCFGVYERATGAQVGFTRVVTDHATFAYLCDVYVLEAHRGHGLGKRMMREVMAHPAI
                     TVARRVMLATRDAHGLYRQSGFVEAGREGNLMEVVRADMYRA"
     gene            complement(5533..5862)
                     /locus_tag="VEW08_16795"
     CDS             complement(5533..5862)
                     /locus_tag="VEW08_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HYJ42451.1"
                     /translation="MKISDLPFGTVDWNTVERITHAGQTGEAQWRTRHFGEVRVRLLE
                     YSAGYLADHWCTKGHFMFCLDGVLETELADGRRFTLRPGMSYHVADGAEPHRSSTPTG
                     ASLYVID"
     gene            complement(5874..6866)
                     /locus_tag="VEW08_16800"
     CDS             complement(5874..6866)
                     /locus_tag="VEW08_16800"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF004771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HYJ42452.1"
                     /translation="MNAVPVIATPSLPDRAAIESAAQFVATLVPPTPQYVWPQVAAAF
                     GAEVWLKHENHTPIGAFKARSAAMYFQKMMQREPGCPGVITATRGNHGQAVGLAARRF
                     KLPATVYVPHGNSVEKNRAMRALGVKLVEHGHDFQESREEAARVGARDGLHMVPAFHA
                     DIVLGVTSYWTELFRAAPDLDVIYVPIGQGSGICAAAAVRNVYSPRTKIVGVVSAHAP
                     TFSKSFAARRVIEAPVETKLGDGMACRKPDDDALAIILANVSRVVEVTDTELAAAMRA
                     IFAMTHNVAEGAGAAAFAAAWRERESLAGGRVGITVSGGNVDTDVFANVLAGKY"
     gene            6948..>7427
                     /locus_tag="VEW08_16805"
     CDS             6948..>7427
                     /locus_tag="VEW08_16805"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYJ42453.1"
                     /translation="MSLQTKQDHRLPAQLAARLAQRIENHALRPGTRLPSIRNCAEES
                     RVSRSTVVEAYDRLIAAGLIESRRGSGFYVRLRNQKAAHRRAATAPLPGTVLDVSWLL
                     LAQRRQSPGSEQPGAGLLPEDWLDHEMVAAAVRGVGRSRGSWLLKYGAVEGYLPLREA
                     "
BASE COUNT         1241 a         2372 c         2450 g         1364 t
ORIGIN      
        1 tacgtacttt ttcgtgtcgg ctgcgtgcga ccttatggtg gtggcgcaac agccatcatc
       61 attcgcacca gctcggccag cgacccggct tccattttct gcatgacgtg cgcgcggtgg
      121 atttccaccg tgcgctggct aacacccagg tctccggcca ccatcttgtt tgcctttccc
      181 tgcgtgacga gatcgagaac ctcgcgttcg cgaggactaa gggacgcaag ccgttcgcga
      241 atccggtccg tgtggcgaag ccgcgcgcgg ttttccgtgt cccgaaccag cgcgcgctgc
      301 acgcgatcca gcaattcctg atcgcgaaat ggtttctgca ggaagtcgaa ggcgccgtgc
      361 tgcatggcct cgaccgccat cggaatgtcg ccatggccgg tgatgaagat gaccggcatg
      421 gtgacaccgc gcaggttcag ctcatgctgc atctcgagtc cgctcatccc cggcatgcgc
      481 acgtcgagca ccatgcaacc cggctgcgac gcgtcgtagc tcgaaaggaa atcctgggcg
      541 gacgcgcaga cttcggcgtg tacaccggcg gatttgagga gcattcgcag cgagtcacgc
      601 accgcatcgt cgtcgtcgac cacgaacacg gtctgcggca ggtctttcat gatggtgcct
      661 cctgatgggc cggaaggacg aagtagaagc atgcgcctcg cggctgattg gcgcgatacc
      721 cgagacgggc gtcgtgggcc ttgatgatgg accggctgat cgcaaggccc agcccggtcc
      781 ccgacgcttt ggtggtgaag aacggttcga aaatcttctg ttccgcttcg ccggccaggc
      841 caggcccgag atcgctgacc gacacctcga cgtctcccgt gtcagtacaa cgggtcgcta
      901 ttcgcacttc aaatggcgcc ccgtcatgtt cgggcgcctg ggcttcgaat gcgttgtgca
      961 ccaggttcat gaggacctgc tggatctgca cgccgtccac cgtcacgttc ggcaacggcg
     1021 acgccaggtc gagcgccagc ttgccgtcgt gcacgcgcgc atcgctcgcc agcagcccgt
     1081 gaacctcacg gatcagctcg ttgacgtcct gcgtctcgcg ccgcacgtcc tcgttgcgta
     1141 ccagtgaacg aatgcgccgg atgatttcgc cggcgcgcag ggcctggctc gcaatctgtt
     1201 cgagcgcccc ggcgatctcg ggcaggtcat gcgttggcaa gcgcaggaac cgtacgcagg
     1261 cctgggcata gttggcgatg gccgcgagcg gctgattgag ttcatgggaa atccccgccg
     1321 ccatttcacc catcgtcgcg aggcgcgcga cgctgttcat gcgctcgagc gcccggtgcg
     1381 cttcgctttc ggcgcgccgc tggtcggtga tgtcctcacc ggagagcatg acaccggtcg
     1441 cgaagttgtc ggcatcgcgc agcgccacgc cgcgccacgt gatgaagcga tcttccccat
     1501 cagacgagcg aacgtggtaa tcgcacacct gcggttcgtc atttccgctc atcagcaggc
     1561 tgcgaaacgc tgcgcgcgcg gtgctgcgat cttccggggc cacgcaactt tcgagccagt
     1621 tgctgtcgac gatcgccgcg tgctcgcgct tgagcacgcg gatggcgcgc tgattggcca
     1681 gctgcaccag cccattgcga tccgtagcca ccagcatcac ctgggccagg tccaggtaga
     1741 gccgattgac ctggcgctcc ttttcgaggg tcgccagcgc tttctggcgc aacgaggtgt
     1801 cgtgcaaaaa ccccacgaat cgtggcgggg aggtgttcgg aattcggccg acgctcagga
     1861 agaccgaaaa ttcgctgcca tcccgatgct gtacccgcac gtcgcggcca cggcccagca
     1921 cgcgcgcctc gccgcccgcc aggtaacgtc tgaggtggtc gtcgtgtccg atgcgatcca
     1981 ccgcggtcat cagcatgctg acattgcggc cgagggcgtc ggcggcgcgg taaccgaaca
     2041 tgacttcggc tgaggcgttg aatgcctcga tgcgcccgcg atcgtccatg atcacgacgg
     2101 cgtcgagcgt agcctccagg agtgcgagaa cttcgggtgt cggcgcggtc atgccacgca
     2161 aagatactcc ttgcgcggcg tgggctgtga gcaggtcgac tggttaggct cggctgccgg
     2221 ctttcgcgga catcagcctt gcatcgactt ccgactcggc cgcgagccag tcttccatct
     2281 catggcccgg atcgaagccc cgctgctcgg cccggaagaa cgcggcctcg gcgatcagcg
     2341 ccgcgcggcg ttcggggtcg acgaagcggg tgagggttgg cctgctctcg ccggtgtcgg
     2401 tggtgacggt cttgcgggac gtgcgtttcc gcacggtggg ggtcgccgga tttttctcac
     2461 gtggcatcgc gagcgctcct cggggtttgt atgaatgctt gcaggaacag ggacctgcgc
     2521 gattgtctcc attgagacag ctccaggcgc gcagtattaa cagtgacgag cacgagtttt
     2581 tttcatgtgt gcgacgttgt ggcggctcta accactttct tggccactgc cagttttcag
     2641 cccggcagga tgggcatgga caggatctgg acgccgcgcc ccgtgcgtgg cacgcgcgaa
     2701 gcgaaatcgg cgggtttcac caccaggatg tcggcggtga ccgcatccat gacgaactcc
     2761 gccgtgttgc cgatgaaaaa gcgcctgagg ccggaacgcg acacgatccc catggcaacg
     2821 atggcgctgc gttcgcgctt ggcggcgccc ggaatggctt cgttcgggcg tccttccacc
     2881 aggtggcagt ggctgcgctt cacatcgaat ccgtcgaccg cccgctcgag cttgcgtcgc
     2941 gccgcgcgtt cgagatcgcg ttccgcggcc acgtcaatca tgagtccgtc gggcatggcc
     3001 ggaaccacgg gtaccccggc gtggaacaca tgcagcgcat gcagctcgcc acgcagtgca
     3061 tgcaccagct gcgctccggc gcgcaggatc tggcggtcga ggttgctcgg cttggcgaac
     3121 gcgtgtgaag gatccacggc cgcgagtact cgcggccggt gatagaggcg cctgtttttc
     3181 accagcagga ccggaatggg gcagaggcgc agcagttccc agtcggtgta cgcgagcagc
     3241 cagcgcgccg gatgccggcc ggtgccgtga tggttttcga cgaccagcag gtcggcggcg
     3301 aggcgttgcg acttgcggat gatggcttcg aagggcggat agtcccagtc agcgacgaca
     3361 tcgacggcaa tgccgtgctt gcggatgcgc acggcgagtt tttcgagctg ctcgcgtcgg
     3421 gcggtttgca cctcgcgctg ccacgacggc aggtcgaact cccgaccctg catcgcctcg
     3481 gcatacaacg gcgtagcgat gtcgtgaaac aacgtgagct gcgcgcccag ccccttggcg
     3541 atctgcgcag ccttgttcac cgcgggcagc acgcgcgccg tgggattttt gacagccacg
     3601 agtattcgcc ggatcggtcg catgacgcga gcgtcgcgca cgcgcagcca ctactgcatc
     3661 ggggaaatta cggataccgc tgtcggtgag cgcctacttg tcgcttttgt tcgctgactt
     3721 cgccgattcc tccagaaata cgcgcagttc cgatgattcg tcgaccagca gcttcgcggc
     3781 ctggcgcagc cgggctgtct gttcggatga gatggtgaac gcggtcggta cgaggtccag
     3841 gtacttgcgc tctgccggat ccttgacccg ctcgaacgag agttcgacga ggtcgacgtc
     3901 gaatttttcg cagtcaggaa ttgccagcgc cacattgcag cgggtgttgc gcacctcgct
     3961 ctcccagttc ttcacgcgcg cgcgcatcag gttcatggtt tcggagctgt agcggttgaa
     4021 cgtgacgctg accacggcgt ccatgacttt cttgatgcca aggggagtcg gccgctgcgc
     4081 gtgggtttcg tcgaagcccg tgcgcgcgtc taccacgata aacagcatgc gccgtgcacg
     4141 cgcaaagccg ttgcggcgca gcgcgtcggt aacggtgtcg cccagcgcca cctcatcgag
     4201 gatggcgcgc gcaccgaggt tatccgcgaa ggcgccgtcg gccaggtgca ggtatggcag
     4261 gtgttcggaa tcgaggtagg acaacagtcg tttggcgcgc tcgcggcggt agcggtctga
     4321 ttcggcggca gcgtcggtca cactctccgg cggtacgtac ccgcattgtc cggcgaacgt
     4381 ctgcatcacc agcggcgcga aatagatcgg cgtcgcggcc gatgcgaaca cggcacgcgc
     4441 gaccggcacg ctctttggat ccacgcagat gccgttcagc tgttcgcgcg tgaacgcgaa
     4501 gggctgcgca cgcaccaggt ccgtcgcgga gatgatgacc cgcggtgagc gcgtcatgat
     4561 gtcgtagacg gtggcgccgt tgaacagctc ctcgtcgaag tactccacct cgaggtcgct
     4621 gcggctgacc agcggtttgg cgagccgtgg ccagctcgtg ggtttgagca ctttgtgcca
     4681 gaggtcctct tccgtgtcgt ggtcgaggaa cctcggcgcg tagctgtcga aggcttcttt
     4741 tccgaacaac gcgtagtagg cagccgtgaa cgcgccgccg gataccgcgg atatcaggtc
     4801 gatctcatcg agcagtgaac gctgctcgcc gtcgggcgtg gtgaccttca cgtcacgcag
     4861 tgcttcgagc acgccgaacg agaacgcggc ggcacgtttg ccgccgccgg agaacgtgac
     4921 gacgacgaaa agcttatcgg tgttggattc gactcctggc gcgagcaccg gggccgtcaa
     4981 aggaacgatt tgcttcagcg gtcgctcgcg ggtgggtgtg ctgccgcagg cagcgagggc
     5041 aagcagggct ccggccgcga tcggccagga aatcgatctg gagaaaatca tgcggcgcag
     5101 tatacggcgc gtcaggcgcg atacatgtca gcgcgcacga cctccatgag attgccctcg
     5161 cgtcccgcct cgacgaagcc cgactggcga tagagcccgt gcgcatcgcg cgtggcgagc
     5221 atgacgcggc gtgcgaccgt gatcgcggga tgcgccatga cttcacgcat catgcgttta
     5281 ccaagcccgt ggccgcggtg cgcttcgagt acgtatacgt cgcacaggta cgcgaacgtc
     5341 gcgtggtcgg tgaccacgcg cgtgaatccc acctgtgcgc ccgtggctct ttcatacaca
     5401 ccgaagcaca gcgagttccg ggcggcgcgt tcgacggtgg tcagcggaat tccgggcgac
     5461 cagtacgagc gcgtgagata ggcgtgaatc gcctcgatgt cgagccgcgc gggatcggtg
     5521 gagatttcgt actcagtcga tgacatagag acttgcgcca gtcggtgtgg aagagcgatg
     5581 cggctcggcg ccgtcggcca cgtgataact catgccgggc cgcagcgtga agcggcggcc
     5641 atcggcgagc tcggtctcga gcacgccgtc caggcagaac atgaaatggc ccttcgtgca
     5701 ccaatggtcc gccaggtagc ccgcggaata ctccagcagg cgcacccgca cctcgccgaa
     5761 atgccgggtc cgccattgcg cctcgcccgt ctggcccgca tgcgtgatgc gctccacggt
     5821 gttccagtcg acggtgccga aaggcaggtc ggagattttc atcgcgtggt cagtcagtac
     5881 ttgccggcga gcacgttggc aaatacatcg gtgtcgacgt tgccgccgct caccgtaatg
     5941 ccgacgcgcc cacccgcgag cgactcgcgc tctcgccagg ccgcggcgaa cgccgcggcg
     6001 cccgcacctt cggccacgtt gtgcgtcatt gcaaagatcg cgcgcatcgc ggccgcgagc
     6061 tcagtgtccg tgacctccac cacgcggctc acgttggcca gaatgatcgc gagcgcgtcg
     6121 tcgtcgggct tgcgacaggc catgccatcg cccagcttcg tttccacggg cgcctcgatg
     6181 actcgccgcg ccgcgaagga tttcgagaac gtcggcgcgt gcgccgatac cacgccgacg
     6241 attttcgtgc gcggcgaata cacattgcgc acggcggccg cggcgcagat tccagagccc
     6301 tggccgatgg gcacgtagat cacatccagg tccggcgccg cgcggaacag ctccgtccag
     6361 tagctggtga cgcccagcac gatgtcggcg tgaaacgccg gcaccatgtg caggccatcg
     6421 cgtgcgccga cgcgcgcggc ttcctcacgc gattcctgaa aatcgtggcc gtgctcgacg
     6481 agcttcacgc cgagcgcgcg catggcgcgg tttttttcca cgctgttgcc gtgcggaaca
     6541 tagaccgtgg cgggtagttt gaagcggcgc gcggcgagcc ccacggcctg cccgtgattt
     6601 ccacgagtgg cggtgatgac gccggggcat ccgggttcgc gctgcatcat cttctggaaa
     6661 tacatcgcgg cactgcgcgc cttgaatgcg ccgatgggcg tgtggttttc gtgcttgagc
     6721 cacacctcgg cgccgaatgc cgcggccacc tgcggccaga cgtactgcgg ggtaggcggg
     6781 accagcgtgg cgacgaattg cgctgccgat tcgatggcgg cgcgatcggg aagggaaggc
     6841 gtagcaatca ccggtactgc gttcatggcc tgaaatatac gtgacgggat cagtacagtt
     6901 acagtacagt tatcaaccca aaacatccga ctggcccgct cttatatatg tcgctgcaga
     6961 ccaaacaaga tcaccgcttg ccggcccagc tggccgcgcg actcgcacag cgcatcgaga
     7021 accacgcgct tcgcccgggt acgcgcctgc cctccatccg caactgcgcc gaggaatcgc
     7081 gcgtcagccg ctcgacggtg gtcgaagcgt acgaccggct cattgccgcc ggcctcatcg
     7141 agtcccgccg tggctccggt ttctacgtgc gcctgcggaa tcagaaggcc gcgcatcggc
     7201 gcgcggccac ggcgccgctg cccggcaccg tgctcgacgt gagctggttg ctgctcgcgc
     7261 agcggcggca gtcgcccggc agcgagcagc ccggcgcggg cctgctgccc gaagactggc
     7321 tggaccatga aatggtcgcg gccgccgtac gcggcgtggg gcgcagccgt ggctcgtggc
     7381 tgctcaagta tggtgccgtc gagggctatc taccgttgcg cgaggcg
//
LOCUS       DASQJS010000426         4982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2793240, whole genome shotgun sequence.
ACCESSION   DASQJS010000426 DASQJS010000000
VERSION     DASQJS010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793240"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /gene="guaB"
                     /locus_tag="VEW08_16810"
     CDS             <1..1363
                     /gene="guaB"
                     /locus_tag="VEW08_16810"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HYJ42454.1"
                     /translation="REIRLNLPLVSAAMDTVTEARLAITMAQEGGIGIVHKSMTIAGQ
                     AAEVARVKKFESGVIRDPITVAPDMTIRQVIELTRSKGISGVPVVQGSKVVGIVTHRD
                     LRFEVKLDAPVSTVMTPKERLVTVRENAPKDEVLLLLHKHRIEKVLVVNSDQELRGMI
                     TVKDFQKATEFPRACKDPSGRLRVGAAVGTAQDTLERVAALREAGVDVVVVDTAHGHS
                     KGVLDMIARIKAQWGDLQIIGGNVATAEAARDLVKAGVDGIKVGIGPGSICTTRIVAG
                     VGVPQISAVSNVAAAVDNKVPVVADGGIRFSGDIAKAIVAGADCVMIGGLFAGTEESP
                     GDVELYQGASYKAYRGMGSLGAMAERHGSADRYFQDTAAELEKLVPEGVEGRVPYKGS
                     LVSIIHQLAGGLRAAMGYTGSTSIDAMRKNPKFVRVTGAGMRESHVHDVQITKEAPNY
                     RVS"
     gene            1387..2970
                     /gene="guaA"
                     /locus_tag="VEW08_16815"
     CDS             1387..2970
                     /gene="guaA"
                     /locus_tag="VEW08_16815"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HYJ42455.1"
                     /translation="MSPQTDIHSSRVLILDFGAQYTQLIARRVREQGVYCEILPWDVT
                     SDDVRKWKPKGIILSGGPESVTDKEPPRAPQAVFELGVPVLGICYGMQTMAQQLGGKV
                     LPSNHREFGYAEVASIAPNRLLDGLFDRRDGQGRAVLDVWMSHGDRVESLPPGFTAVA
                     QSANAPLAAMVDEARRFYAVQFHPEVTHTLQGAKLLERFVREICGCSPLWSVGNIIDD
                     AIARVRAQVGKGKVLLGLSGGVDSSVVAALLHKAIGGQLTCVFVDHGLMRLNEGDQVM
                     KIFADNLGVKVIRVDAEARFLEALRGETDPEKKRKIIGGLFVKVFDEEAQKLTEVDWL
                     AQGTIYPDVIESAGSKTGKAHVIKSHHNVGGLPANMRMKLVEPLRELFKDEVRRLGVE
                     LGLPREMVYRHPFPGPGLGVRILGEVRKDYADLLRQADAIFIEELRRHDLYDKTSQAF
                     AVFLPVRSVGVMGDGRRYDYVVALRAVETVDFMTAHWARLPYDFLDVCSRRIVNEVKG
                     ISRVVYDISGKPPATIEWE"
     gene            3081..3497
                     /locus_tag="VEW08_16820"
     CDS             3081..3497
                     /locus_tag="VEW08_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42456.1"
                     /translation="MSSFIYDLITLLGKKSDDPEVVRAIEKHQLNDVYDDPPLWRYVG
                     SKVKGTDLLFEEECVVDVQVHAQATKRRDAIPGELPFGLKKGMTQRQVHAELGTPYEA
                     DTHDSRYRIANPAIRLLVAYDESGVVRYLSIAPPEV"
     gene            3703..4677
                     /locus_tag="VEW08_16825"
     CDS             3703..4677
                     /locus_tag="VEW08_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42457.1"
                     /translation="MRIVCLGVRRWVWVLLALASCNAFAGIGDGWFARSEFGKEPTCA
                     LFGRTAVDLQTAMSVTIVWTASEGPVVRLNGGLSGIPSVLLESVGTDDRWNVTVDYLR
                     QPEFAARQIMISAGDLDRLIDNIGLGHALAITVANSGKAPLRYLDAPDGRKVAVAMYR
                     ACVKSMAASPPPAYSKWPGMYLFQMADDSECGFRQIFSERSFPPYITLWVSERGGRIA
                     FMRETVERKPHGRIGKRRKTPDRVNSEALFGQTFDLVESFEFDLTLSQVDTLAADLAR
                     GVARNVVMTSPEGESLTLGFGGAYGKASAAMLAACREVKFGSAEKLNP"
BASE COUNT          928 a         1509 c         1650 g          895 t
ORIGIN      
        1 gcgtgaaatc cggctcaacc ttccgctcgt gtccgctgcc atggacacgg tcaccgaggc
       61 gcgcctcgcc atcaccatgg cgcaggaagg cggcatcggc atcgtccaca aatccatgac
      121 catcgccggc caggccgccg aagtcgcacg cgtcaagaaa ttcgagtcgg gcgtcatccg
      181 cgaccccatc accgtggctc ccgacatgac catccgccag gtcattgaac tcaccaggtc
      241 gaaaggcatc tccggcgtgc cggtggtgca gggttccaag gtcgtcggca tcgtcacgca
      301 ccgcgacctg cgcttcgagg tgaagctcga cgcgccggtg tcgacggtca tgacgcccaa
      361 agagcggctc gtcaccgtgc gcgaaaacgc accgaaggac gaagtgctgc tgctgctgca
      421 caagcaccgc atcgagaagg tgctggtggt gaacagcgac caggaactgc gcggcatgat
      481 taccgtgaag gatttccaga aggccacgga attcccgcgc gcctgcaagg acccgtcggg
      541 tcgtctgcgc gtcggtgccg cggtcggcac ggcccaggac actctcgagc gcgtcgcggc
      601 gctgcgtgag gccggagtcg acgtggtggt cgtcgacacc gcgcatggcc attccaaggg
      661 cgtgctcgac atgatcgcgc gcatcaaggc gcagtggggt gacctgcaga tcatcggcgg
      721 caacgtggca acggccgaag cggcgcgcga tctcgtgaag gctggcgtgg acggcatcaa
      781 ggtgggcatc ggtcccggct ccatctgcac cacgcgcatc gtcgcgggtg tcggtgttcc
      841 gcagatctcc gccgtgtcga acgtcgcggc agcggtggac aacaaggtcc cggtcgtcgc
      901 cgacggtggc atccgcttca gcggcgacat cgccaaggcg attgtcgcgg gcgccgactg
      961 cgtgatgatc ggcgggcttt tcgccggtac cgaggaatca cccggcgacg tcgagctcta
     1021 ccagggcgcg tcatacaagg catatcgcgg catgggttca ctcggcgcca tggccgagcg
     1081 ccacggctcg gccgatcgct atttccagga caccgccgcg gagctcgaaa agctcgtgcc
     1141 cgaaggcgtc gaaggacgcg tgccttacaa gggcagcctc gtgtccatca ttcaccagct
     1201 cgcgggtggc ctgcgcgccg cgatgggcta cacgggcagc accagcatcg acgccatgcg
     1261 caagaacccg aagttcgtgc gcgtcaccgg cgccggcatg cgcgagagcc acgtgcacga
     1321 cgtgcagatc accaaagaag cacccaacta ccgagtttcc tgatgaacag tgtcgctgcg
     1381 agtccgatga gtccacaaac cgacatccac tcttcgcgcg tcctgatcct cgacttcggc
     1441 gcccagtaca cccagctcat cgcgcgccgc gtgcgcgagc agggcgtgta ctgcgaaatc
     1501 cttccctggg acgtcaccag cgacgacgtt cgcaagtgga aacccaaagg catcatcctc
     1561 tccggcggcc cggagtccgt aaccgacaag gaaccgccgc gcgcgccgca ggcggtattc
     1621 gagctcggcg tgccggtgct cggcatctgt tacggcatgc agaccatggc gcaacagctg
     1681 ggcggcaagg tgctgcccag caaccatcgt gaattcggtt atgccgaggt cgcgtccatc
     1741 gcgcctaaca ggctgctcga cgggttgttc gatcgcaggg atgggcaggg tcgcgccgtg
     1801 ctcgacgtct ggatgagcca cggcgaccgc gtcgaatcat tgccgccggg attcacggcg
     1861 gtggcgcaga gcgccaatgc accgctcgcg gcgatggtcg acgaagcgag gcgtttctac
     1921 gccgtgcagt tccatcccga agtcacgcac acgctgcagg gtgcgaagct gctcgagcgc
     1981 ttcgtgcgcg agatctgcgg ctgctctccg ttgtggagtg tcggcaacat catcgacgac
     2041 gccatcgcgc gtgtgcgcgc gcaggtgggc aagggcaagg tgttgctggg cctctccggt
     2101 ggcgtggatt catcggtagt cgctgcgctg ctgcacaagg ccattggcgg acagctgacc
     2161 tgcgtgttcg tcgatcacgg cctcatgcgc ctgaacgaag gcgatcaggt catgaagatc
     2221 tttgccgaca atctcggcgt gaaagtcatc cgcgtcgatg ccgaagcgcg cttcctcgag
     2281 gcgctgcgcg gcgaaaccga ccccgagaag aagcgcaaga tcatcggtgg cctgttcgtg
     2341 aaggtgttcg acgaagaagc gcagaagctg accgaagtcg actggttggc gcagggcact
     2401 atctaccccg acgtcatcga gtccgcgggc agcaagaccg gcaaggcgca cgtcatcaag
     2461 tcgcaccaca acgtgggtgg actgcccgcc aacatgcgca tgaagctcgt cgagccgctg
     2521 cgcgagctgt tcaaggacga agtgcgaagg ctcggcgtcg agctcggcct gccacgcgag
     2581 atggtgtatc gccatccgtt cccgggtccg ggcctgggcg tgcggatcct cggcgaagtt
     2641 cgcaaggact acgccgacct gctgcgccag gctgatgcca tcttcatcga agaactgcgc
     2701 cgccacgacc tgtacgacaa gacctcgcag gccttcgccg tgttcctgcc ggtgcgctcg
     2761 gtgggcgtca tgggtgatgg acgccgctac gactacgtcg tcgcgctgcg cgccgtggaa
     2821 accgtggact tcatgaccgc gcactgggcg cggctgccgt acgacttcct cgatgtctgc
     2881 tcgcgccgca tcgtgaacga agtgaaaggc atctcgcgcg tggtgtacga catctccgga
     2941 aagccaccgg cgacgatcga gtgggagtga gcgggcccgg gccacgtgcg ctatcagctt
     3001 aagactctcg agccgctgga cctggtggca cgactggcgc actggtgccg ccatttgatc
     3061 tgcggagcaa ggcccgaccc gtgagctcat tcatatacga tctgatcacg cttctcggca
     3121 agaagtccga cgatccggag gtggtgcgcg ccatcgaaaa acatcaattg aatgatgttt
     3181 acgatgaccc gcccctgtgg cggtatgtcg gctcgaaggt gaagggcacg gatcttctgt
     3241 tcgaagagga atgcgtggtg gacgtgcagg ttcacgccca ggccacgaaa cggcgcgacg
     3301 cgattccggg agagttgcct tttgggctga agaaggggat gactcaacgt caggtccatg
     3361 ccgaattggg tactccttat gaggccgaca cgcatgacag ccgctacagg attgcgaacc
     3421 ccgcgattcg attgctcgtt gcgtacgacg agagcggcgt ggtgcgttac ctcagcatcg
     3481 ctccgccgga agtctgaaga cagcagggcc gtcacctggt tccgcggggc ccgcgcgagc
     3541 gaccggtcaa tggttgtagt tagtcaacgt tcaacgatcc gcgatcgctt gtcgcgcggc
     3601 gctgccttcg gcaatatcgg cagtggcgag caggatcagt tcgcggaggc cggcgggtat
     3661 ggcgtcctgc cgtagttagc cgagcaagga ctgggagggt ggatgcgcat cgtctgtctt
     3721 ggcgtaaggc gttgggtgtg ggtgttgctc gcgctggcaa gctgcaacgc attcgcgggt
     3781 atcggagacg gctggttcgc gaggtcggaa ttcggcaaag agcccacttg cgcgctgttc
     3841 ggccgaactg cggtcgatct gcaaacggcc atgagcgtca ctatcgtgtg gacggctagt
     3901 gaaggacccg tcgtcagact gaacggtggc ctgtcgggaa taccgtcggt gttgctcgag
     3961 tcggtcggga ccgacgatcg ctggaacgtg accgtcgact atctgcgcca gcccgaattc
     4021 gctgcgcgcc aaatcatgat cagcgccggc gatctcgaca ggttgatcga caacataggc
     4081 ctcggtcacg ctctggccat caccgtggcg aattccggca aggcgccctt gcgttatctc
     4141 gacgcgccgg atggccgaaa ggtggccgtg gccatgtatc gtgcctgcgt gaagtcgatg
     4201 gcggcgagtc cgccgccggc gtattcaaag tggcccggca tgtacctgtt ccagatggcg
     4261 gatgacagcg aatgcgggtt tcgccaaatt ttttccgagc gatcctttcc accgtacatc
     4321 acgctgtggg tgagcgagcg gggtggaagg attgccttca tgcgggaaac agtcgaaagg
     4381 aagccacacg gccggattgg caagcgtcgc aagactcccg atcgggtcaa ctccgaggcg
     4441 ctgttcggcc agactttcga tctcgtcgag agcttcgaat tcgacctgac gctgtcgcag
     4501 gtggacacac tggctgccga cctggcgcgc ggcgtggccc gcaatgtggt catgacatcg
     4561 cccgaaggtg agtcattgac cctgggattt ggcggggctt acggcaaggc atcggcggcg
     4621 atgctggccg cctgtcgcga ggtcaaattt ggttcagcgg agaagctgaa tccgtgaact
     4681 atcgaaagat ggcgcggctg gcgcatgcgg ggtgagaatc cagccgatca catcgatgag
     4741 ttgctcgacg acagtgacca ctatccgggc ctcgcggtgt tcctgaaaca ttccagcgga
     4801 gtcgtgcctg aagaactgct tcgggtgcgg ggcctcacgg gagtgacgct gccgtcgttc
     4861 aatcgtggtt tggggggatg tgctgttcac cggagtgttg ttttccgatc gcgagtcgtt
     4921 ccgcaaacac gaggatgcgg tcaagcatct gcatcgtgag tcgaagcacc aactgcgaac
     4981 ag
//
LOCUS       DASQJS010000427         8634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_590358, whole genome shotgun sequence.
ACCESSION   DASQJS010000427 DASQJS010000000
VERSION     DASQJS010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590358"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1545
                     /gene="purF"
                     /locus_tag="VEW08_16830"
     CDS             37..1545
                     /gene="purF"
                     /locus_tag="VEW08_16830"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HYJ42458.1"
                     /translation="MCGIAGIVGTSGVNQRIYDALTVLQHRGQDAAGIATVAGGELFT
                     RKGRGLVRDVFEQRHMEELKGNIGMGHVRYPTAGCDSAAEAQPFYVNAPYGICLAHNG
                     NLTNARELGEVLEREDRRHLNTSSDSEVLLNVLASELQRVGTPRVTPADIFAAVNAVY
                     RRCRGGYAVVAMVIGHGLLGFRDPNGIRPLVLGQRETPKGAEWMLASESVALDMLGFK
                     LVRDVAPGEVVYIDTHGRLHSQVCVATAKHAPCIFEYVYFARPDSLIDNISVHRARMR
                     MGERLAEKILRERADHHIDVVIPIPDTSRTSAMQVAQRLDVKYREGFVKNRYVGRTFI
                     MPGQEQRSRSVRSKLNAIDLEFRNKNVLLVDDSIVRGTTSAQIIDLAREAGAKKVYFA
                     SAAPPVKFPNVYGIDMPAASELVAANRTETEVAQLIGADWLIYQDLEDLIQACVHDHS
                     SIREFDTSCFSGHYVTGDVTPEYLARLQSERSDMAKASRRDGDKAGLKIVKS"
     gene            1576..2760
                     /locus_tag="VEW08_16835"
     CDS             1576..2760
                     /locus_tag="VEW08_16835"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HYJ42459.1"
                     /translation="MNARRILLDVFDVALRAVDGCACVDRFLRDAELPGPVEVFAVGK
                     AASSMARGAHVALGSRVERMLVITKDGHTDPALDSVDGVMQLQSAHPVPDLRSFSNGI
                     ELEERVRNLRDDHFPLFLISGGSSSLVEALRDGVSLEEVRALNVDGLAGGWDITRLNL
                     ARARLSRIKGGGIARLLGGRRALALFISDVPGDDPDVIGSGLLGHAAGIADGIERHVI
                     ATIETAVGAVRDAARAHGLTFEVMPARFAGNAGEVAAQLLERLRAGDCDGVVWGGEST
                     VTLPSSHGRGGRNTHLALSFARQLRVGEPWMLLAAGTDGTDGPTSDAGAIVDAGTTER
                     ASLAGCDVERAWREFDSGVALEAAEDLVHTGPTGTNVGDILIAIKQSATSLRGQPPPR
                     ML"
     gene            2769..4040
                     /locus_tag="VEW08_16840"
     CDS             2769..4040
                     /locus_tag="VEW08_16840"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYJ42460.1"
                     /translation="MRLMLIDDDPRYRTLLRHHISCAWPDIELVSYNPRVRGPLTPGF
                     LAQGYSAVLLDHAWKGGSGLDWLKDFHDREGFAPVIFLSAEDESADAVEARATGAFEV
                     IGKTKIKHTKLNDAIRRAADEQAKAQSRWRMSAGAKMSQDFAGARLKEYRRIELIAKG
                     SVSELFLAESARDGDVVVLKVTPAIRKETGVDQSMERFLQEFEMLREIQHPNIVRIYD
                     LGVTDDHLFLAMEHFARGDLRKRMSEGLTARQSLAYARDLAYALQAIHEVGIFHRDLK
                     PGNVMLRDDGSIALIDFGLAKHVALKMEVTDKGLIFGTPHYMSPEQGHGKEIDARSDL
                     YALGVMLYEMLTGKKPFDADNHMAILVHHAKAPIPRLPERIASVQPLIDTLMAKDAAD
                     RPANADEAAKQIDAALVALTAITTLTPADPA"
     gene            4037..4696
                     /locus_tag="VEW08_16845"
     CDS             4037..4696
                     /locus_tag="VEW08_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020796318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HYJ42461.1"
                     /translation="MSEKREARAAARFERATDRSILKAVTPRAWVDAALADLPTLLVD
                     HANCEKKAASTAMALIFAYPEDRSLSVALSRLAREELKHFEQVERLMRKLSVPYQRMK
                     PGRYASELRKLVRNHEPKRKLDLMVVHALIEARSCERFRLLAKRLPEQVRDLYEQLER
                     SEARHFELYLDFAGREFEADEIAGRLEVIATREAELATSADHELRFHSGPPEMGSGTV
                     S"
     gene            complement(4718..5452)
                     /locus_tag="VEW08_16850"
     CDS             complement(4718..5452)
                     /locus_tag="VEW08_16850"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HYJ42462.1"
                     /translation="MSVAAHSLFASDLHLDSESPWAIDAFLAFLDGPARSAEALYLLG
                     DLFEVWVGDDDDNPDNTRTCAGLARLTASGVAVYVLHGNRDFLMGEAFAQRTGAKLLP
                     DPVLLDLYGVPTLLSHGDVFCTEDPAYQALRSIVRQPGWQRRFLALPLAARRTLASAA
                     RAGSKAHTQRTVPTIMDVNPEAVTRAMRATGARRLIHGHTHRPAVHPFVVDEVSVERV
                     VLAPWYEAASCLGVSAEGVREIPLPR"
     gene            complement(5449..6147)
                     /locus_tag="VEW08_16855"
     CDS             complement(5449..6147)
                     /locus_tag="VEW08_16855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYJ42463.1"
                     /translation="MKKPFLLLALLGLLTYQARAANIERVRVTTNFGAFVVEMQRDRA
                     PLTVENFLFYVKSGYYTNTLFHRVISGFVIQGGGVGLDYKAKPTQKPIANEAGNGLKN
                     LRGTVGLARASGPHSGDCQFYVNVADNADLDPLPTRWGYAVFGKIVEGMEVLDRISVS
                     PTGSMGPFKQDAPLQPVVIQKIELITEQTTVTPPPAPATAAPAEGATPPDEASKGAAP
                     AGAEPPPQTAPPPK"
     gene            6204..7610
                     /gene="gltX"
                     /locus_tag="VEW08_16860"
     CDS             6204..7610
                     /gene="gltX"
                     /locus_tag="VEW08_16860"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HYJ42464.1"
                     /translation="MSQGPITTRFAPSPTGELHLGNARTALFSWLFARHHGGRFVLRI
                     EDTDSERSKESYTTALMEDLQWMGLPWDAGPERAQAGEEYRQSRRGQVYARHSATLDA
                     SGLTYPCYCTPLELDLSRKAQLAAGRPPRYAGTCRELAPEARVRKSAQGISPTTRFRV
                     PMGRRVEFDDMVHGAQSFATDDIGDFILRRADGSAAFFFSNAIDDAEMGITHVLRGED
                     HLTNTPRQLLVLEALKLRAPTYGHLSLLVGADGSPLSKRHGATSVREFRERGYLPVAL
                     ANHLFRLGHSSSENGVLTLDGMARAFAVKHLGRAPARFEESQLNVWQREVAHHMPLEE
                     SERWLAAETPGDIDPGRRRAFIAAIRPNVLLPSDVARWRDVVFGAAPALDEPSLARVR
                     EAGVQFFRAAADAAATGGKLDAIVSAVKAATGAKGQALWKPLRLALTGAPEGPELAPL
                     LLAMPDSAIHDRLSRFAA"
     gene            7607..>8634
                     /gene="cysS"
                     /locus_tag="VEW08_16865"
     CDS             7607..>8634
                     /gene="cysS"
                     /locus_tag="VEW08_16865"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HYJ42465.1"
                     /translation="MIRIHNSLTGVKQELKPVEPGHVRMYSCGITVYDYFHVGNARML
                     VVFDMVSRYLRYRGLRVTYVRNITDVDDKIINRAKESGVDWRAHAAKYTAAMHEDLDR
                     LGCLRPEKEPKASDYIDEMLTMIGQLITNGYAYVASDGDVMYAVHKFAPYGKLSGKKL
                     EDLRAGSRVEVDTTKRDPLDFVLWKSAKPGEPAWPSPWGDGRPGWHIECSAMSTRELG
                     DHFDIHGGGLDLKFPHHENEIAQTCGATHGRFAEIWMHNGFLNIDNEKMSKSLGNFFT
                     TREVLAKIKHPEVLRFFLLSSHYRGPMNYSPDQLDQAEAGLNRLYTALRDVPEVGGDL
                     YSPTAATQV"
BASE COUNT         1534 a         2830 c         2834 g         1436 t
ORIGIN      
        1 ccaaaccctc cgtctccacc tgaggatctg aaccgaatgt gcggcatagc cggaatcgtc
       61 ggaaccagcg gagtcaatca acggatctat gacgccctga cggtgctcca gcaccgcggg
      121 caggatgccg ccggcatcgc caccgtcgcc ggaggcgaac tgttcactcg caaggggcgg
      181 ggtctcgtcc gcgacgtatt cgagcagcgc cacatggaag agctcaaggg caacatcggc
      241 atggggcacg tgcggtaccc gaccgccggc tgcgacagcg ccgcggaagc ccagcccttc
      301 tatgtgaacg ccccctacgg catctgtctc gcgcacaacg gcaacctcac caacgcgcgc
      361 gagctcggcg aagtgctcga gcgcgaagac cgccgtcacc tgaacaccag ctccgattcc
      421 gaggtgttgc tcaacgtgct ggcctcggag cttcagcgcg tgggtacgcc gcgcgtgaca
      481 cccgccgaca ttttcgccgc ggtgaacgcg gtgtatcgcc gctgccgcgg aggctacgcg
      541 gtggtcgcca tggtcatcgg ccatggcctg ctcggatttc gcgatcccaa tggcatccgg
      601 ccgctggtgc ttggccagcg cgaaacgccc aagggcgccg aatggatgct ggcgtccgag
      661 agcgtcgctc tcgacatgct cggcttcaag ctggtgcgcg atgtggcgcc gggcgaagtg
      721 gtgtacatcg acacgcacgg ccggctgcat tcgcaggtat gcgtggccac cgccaaacat
      781 gccccctgca tcttcgaata cgtgtacttc gcgcgacccg actcgctgat cgacaacatc
      841 tcggtgcacc gcgcgcgcat gcgcatgggc gaacggctcg cggaaaagat cctgcgcgag
      901 cgcgccgacc atcacatcga tgtggtcatt cccattccgg ataccagccg caccagcgcc
      961 atgcaggtcg cgcagcgcct cgacgtgaag taccgcgaag gcttcgtcaa gaaccgttac
     1021 gtgggtcgca cgttcatcat gccgggccag gagcagcgct cgcggtcggt gcgaagcaag
     1081 ctcaacgcca tcgacctcga attccgcaac aagaacgttc tgctggtcga cgattcgatc
     1141 gtgcgaggaa cgacctccgc gcagatcatc gatctcgcgc gtgaagcggg cgcgaagaaa
     1201 gtctatttcg cctcggccgc gccgccggtg aagtttccga acgtgtacgg aatcgacatg
     1261 ccggccgcga gcgaactcgt ggcggccaat cgcaccgaga cggaagtcgc gcagctcatc
     1321 ggcgccgact ggctgatcta ccaggatctc gaagacctca tccaggcatg cgtgcacgat
     1381 cattccagca tcagggaatt cgacacgtca tgcttctcgg gtcactacgt caccggcgac
     1441 gtcactcccg agtacctcgc acggctgcag tcggagcgat ccgacatggc caaggcatcg
     1501 cggcgcgatg gcgacaaggc cggcctcaaa atcgtcaaga gctgagcgtt ccccgggcga
     1561 acgcggcccg ctggcatgaa cgcgcgccgc atcctgctcg atgtcttcga cgtggcgctg
     1621 cgcgccgtcg acggttgcgc ctgcgtcgac cgttttctcc gcgatgccga actgcctggt
     1681 cccgtcgaag tgtttgccgt gggcaaggcc gcgagctcca tggcgcgcgg ggcgcatgtc
     1741 gccctggggt cgcgagtcga gcgaatgctg gtcatcacga aagacgggca caccgaccct
     1801 gcactcgaca gtgtggatgg cgtcatgcag cttcagagcg cgcatcccgt gcccgacctg
     1861 cgcagcttca gcaatggcat cgagctcgaa gagcgcgtgc ggaatcttcg tgatgaccat
     1921 tttccgctgt tcctcatctc gggtgggagc tcgagcctcg tggaagcgct gcgcgatggc
     1981 gtctcgctcg aagaggtgag ggcgctcaac gtggacggac tcgccggcgg ctgggatatc
     2041 accaggctga acctcgcgcg cgcacgcctg tcgcgcatca agggcggtgg aattgcacgc
     2101 ctgctgggcg gtcgccgtgc gctcgcgctc ttcatctcgg acgtgcccgg cgacgatccg
     2161 gacgtgatcg gttcggggtt gttaggtcac gccgccggga ttgccgacgg catcgaaagg
     2221 cacgtgatcg ccaccatcga gaccgcggtt ggcgcagttc gggatgccgc gcgcgcgcac
     2281 gggctcacgt tcgaagtgat gcccgcgcgg ttcgccggca acgcgggcga ggtggccgcg
     2341 cagctcctcg agcgactgcg agccggcgat tgcgatggag tggtgtgggg cggcgaatcc
     2401 acggtgacgc tgccgtcaag ccacggtcgc ggcggacgca atacccacct cgcattgagc
     2461 ttcgcgcgac aactgcgtgt tggcgaaccg tggatgctgc tggcggcggg taccgacggc
     2521 accgacggac cgaccagtga tgcgggcgcc atcgtcgatg cgggaacgac tgagcgcgcc
     2581 tcgcttgcgg gctgcgatgt cgagcgcgcg tggcgcgagt tcgattccgg cgttgcgctc
     2641 gaggcggccg aagacctcgt gcacaccgga cccaccggca cgaatgtcgg ggatatcctg
     2701 atcgccatca aacaatccgc gacctcgtta cgtggccaac ccccgccacg catgctctaa
     2761 tccgcgcgat gcgcttgatg ttgatcgatg acgacccgcg gtaccgcaca ctgctgcgtc
     2821 accatatttc ctgtgcctgg cccgacatcg agctggtgag ttacaacccg cgcgtgcgcg
     2881 ggccgctgac accgggattc cttgcgcagg gttacagcgc cgtgctgctc gatcacgcat
     2941 ggaaaggcgg ctcgggactc gactggctca aggatttcca tgaccgcgag ggattcgctc
     3001 cggtcatttt cctgtccgcc gaagacgaat ccgccgatgc ggtcgaagca cgcgcgaccg
     3061 gcgcgttcga ggtcatcggc aagaccaaga tcaagcacac caaactcaat gatgcgatcc
     3121 gccgtgccgc cgatgagcag gccaaggcgc agagccgctg gcgcatgtcg gccggcgcga
     3181 agatgtccca ggacttcgca ggggcacggc tcaaggaata ccggcgcatc gagctcatcg
     3241 ccaagggttc ggtgtcagag ctcttcctgg ccgagagcgc acgcgacgga gacgtggtgg
     3301 tgctcaaggt cacgcccgcg atccgcaagg aaacgggcgt cgaccagtcg atggagcgct
     3361 tcctgcagga attcgagatg ctgcgtgaaa tacagcatcc caacatcgtg cgcatctacg
     3421 atctcggggt caccgacgat cacctgttcc tcgcgatgga acactttgcg cgcggcgacc
     3481 tgcgcaagcg catgtccgag ggcctgaccg cacggcagtc gctggcctat gcccgcgatc
     3541 tcgcctacgc gctgcaggcc attcacgagg tcggcatttt tcatcgcgac ctgaaaccgg
     3601 gcaacgtgat gctgcgagac gacggcagca tcgcgctcat cgacttcggt ctcgccaagc
     3661 atgtagcgct caagatggaa gtcaccgaca agggcctgat cttcggcacg ccgcactaca
     3721 tgagcccgga acaggggcac ggcaaggaga tcgacgcgcg cagcgacctg tacgcgctcg
     3781 gcgtcatgct gtacgaaatg ctgaccggca agaaaccctt cgacgctgac aatcacatgg
     3841 cgattctcgt gcatcacgcc aaggcgccaa ttccgcgcct gccggaaagg atcgcctccg
     3901 tgcagccgct catcgatacg ctcatggcca aggatgccgc cgatcgccct gcgaatgccg
     3961 acgaggccgc gaagcagatc gacgcggcgc tcgtggcgct cacggccatc acgacgctca
     4021 caccggcgga cccggcgtga gcgaaaagcg cgaggcacgc gccgcggcgc gcttcgaacg
     4081 cgccacggac cggtcgattc tcaaggcggt gacaccgcgc gcctgggtgg acgcggcgct
     4141 cgcggatctg ccgacgctgc tcgtcgatca cgccaattgc gagaagaagg ccgcgtccac
     4201 cgcgatggcg ctgatttttg cctatccgga agaccgctcc ctgagcgtcg ccttgtcgcg
     4261 cctggcacgc gaagagctca aacacttcga gcaggtcgag cggctcatga ggaagctcag
     4321 cgtgccatac cagcgcatga aacccggacg ctacgcgagc gaactgcgca agctggtacg
     4381 gaatcacgaa cccaagcgca agctcgacct catggtggtg cacgcgttga tcgaagcgcg
     4441 ttcgtgcgaa cgctttcgcc tgctggcgaa gcggttgccc gagcaggtgc gcgacctgta
     4501 cgagcagcta gagcgctccg aggctcgcca cttcgagctg tatctcgact ttgccgggcg
     4561 cgagttcgag gctgatgaaa tcgccgggcg gctcgaggtc atcgctaccc gggaagccga
     4621 gctcgccacc agcgccgatc acgaattgcg cttccactcc ggcccaccgg aaatggggtc
     4681 aggcaccgtt tcctaggaaa cggtgcctga ccccatttca gcgggggagt ggaatctcgc
     4741 gtacgccctc ggcgctgact cccaggcagc tcgctgcctc gtaccagggt gccagcacca
     4801 cgcgttcgac gctcacttca tcgacgacga atggatgcac ggccggcctg tgggtatgcc
     4861 cgtggatcag ccgccgcgcg ccggtggcgc gcatggcgcg cgtcacggct tcgggattga
     4921 cgtccatgat ggtcgggact gttcgctgcg tatgcgcctt gctgccggcg cgcgccgcgc
     4981 tcgcgagcgt ccggcgcgcc gcgagcggca aggccagaaa gcggcgctgc cagcccggct
     5041 ggcgcacgat gctgcgcagc gcctggtacg cgggatcctc tgtgcagaac acgtcgccat
     5101 ggctgagcag agtggggacg ccgtagagat ccagcagcac cgggtccggc agtagtttgg
     5161 cgccggtgcg ttgcgcgaac gcctctccca tcaggaaatc ccggttgccg tgcaacacgt
     5221 acacggcgac gccgctcgcc gtcaggcggg cgaggccggc gcaggtgcgg gtgttgtcgg
     5281 ggttgtcatc gtcgtcgccc acccagactt cgaacaggtc gccgagcagg tagagcgctt
     5341 cggcggaacg cgccggcccg tcgaggaagg cgagaaatgc atcgatggcc cagggggact
     5401 cgctgtcgag atgcaggtcg gaggcgaaaa ggctgtgagc cgcgacactc acttcggcgg
     5461 tggtgctgtt tggggcggcg gttctgcacc tgccggcgcg gctcccttgc tggcttcatc
     5521 gggcggggtc gcgccctcgg cgggcgcggc agtcgccggc gccgggggcg gcgtcaccgt
     5581 ggtctgctcg gtgatgagtt cgatcttctg gatgaccaca ggctgcagcg gtgcatcctg
     5641 cttgaacggt cccatcgagc cggtgggtga gacgctgatt cgatcgagca cttccatgcc
     5701 ttcgacgatt tttccgaaca cggcgtaacc ccagcgcgtg ggcaggggat cgagatcggc
     5761 gttgtcagcc acgttcacat agaactggca gtcacccgag tgcggcccgc tggcgcgcgc
     5821 gaggccgacc gtgccgcgca gattcttgag cccattgcct gcttcgttgg cgatcggttt
     5881 ttgcgtgggt ttggccttgt agtcgaggcc cactccgccg ccctggatca caaagcccga
     5941 gatgacgcga tggaacagcg tgttggtgta gtagccggac ttcacataga acaggaagtt
     6001 ttccacggtc aacggcgccc ggtcgcgctg catttcgacg acgaaagcgc cgaagttggt
     6061 ggtgacacgc acgcgctcga tattggcggc ccgggcctgg taagtgagca gcccgaggag
     6121 ggcaagcagc aggaatggct ttttcatggc cggcgattct accgaagctg cggtgcgtcg
     6181 ttgctttccc gtaaaatccc ccggtgtcac agggacccat caccactcgc ttcgccccca
     6241 gccccacggg tgagctgcat ctcggcaacg cgcgtacggc gctgttcagc tggctgttcg
     6301 cgcgccacca cggcgggcgc ttcgtcctgc gcatcgagga cacggattcc gaacgcagca
     6361 aggagtccta caccacggcg ctcatggaag acctgcagtg gatgggactg ccgtgggatg
     6421 cgggtccgga acgcgcccag gccggcgagg aataccggca gtcgcggcgc ggccaggtct
     6481 acgcgcgcca ctcggccacg ctcgatgcca gcgggctcac gtacccgtgc tactgcacgc
     6541 cgctcgagct ggatttgtcg cgaaaggcgc agctcgccgc cggccgcccg ccacgttatg
     6601 ccggaacctg ccgggaactc gcccccgaag cgcgcgtgcg caagagcgcc cagggcatct
     6661 cgccgacgac gcggttccgc gtgcccatgg gccggcgcgt ggagttcgac gacatggtgc
     6721 acggcgcgca gagttttgcc accgacgaca tcggcgattt catcctgcgt cgtgccgacg
     6781 gcagcgcggc attcttcttc agcaacgcca tcgatgacgc ggaaatgggc atcacgcacg
     6841 tgctgcgtgg tgaagaccac ctgaccaaca cgccgcgcca gttgctggtt ctcgaggccc
     6901 tgaagctgcg tgcgcccact tacggacacc tgtcactgct ggtcggcgcc gacgggtccc
     6961 cgctttccaa gaggcacggt gccaccagcg tgcgcgagtt ccgcgagcgg ggctatctac
     7021 ccgtggcgct ggcgaatcac cttttccggc tcgggcattc gagcagcgaa aacggcgtgc
     7081 tgaccctgga cgggatggcg cgcgcgttcg cggtcaagca tctcgggcgc gcaccggcgc
     7141 ggttcgagga gtcgcagctc aatgtctggc agcgcgaagt cgcgcatcac atgccgctcg
     7201 aggagagcga gcggtggctt gcggccgaga cgccgggcga catcgatccc ggtcggcgtc
     7261 gcgcgttcat cgccgccatt cgtcccaatg tgctgttgcc gtcggacgtc gcgcgctggc
     7321 gcgatgtcgt gttcggtgca gcgccggcgc tcgacgagcc ttcgctggcg cgcgtgcgcg
     7381 aggccggcgt ccagttcttc cgcgcggccg ccgatgccgc ggccacgggc ggcaagctcg
     7441 acgccatcgt cagcgccgtg aaggcggcca ccggcgccaa gggccaggcg ctctggaagc
     7501 cgctgcgcct cgcgctcacc ggcgcgccgg aaggcccgga actcgcaccg ctgctgctgg
     7561 caatgcccga ttccgccatt cacgatcgcc tgagcagatt cgccgcatga tccgcatcca
     7621 caattccctg acgggcgtga agcaggaact caagcccgtc gagcccggcc acgtgcgcat
     7681 gtacagctgc ggtatcacgg tctacgacta cttccacgtg ggcaacgccc gcatgctcgt
     7741 ggtgttcgac atggtgagcc gatacctgcg gtaccgcggg ttgcgcgtga cctacgtgcg
     7801 caacatcacc gacgtcgacg acaagatcat caaccgcgcg aaggaatcgg gagtcgactg
     7861 gcgggcgcat gcggcgaaat acaccgcggc catgcatgaa gatctcgaca ggctgggttg
     7921 ccttcggccc gagaaagagc cgaaggcttc cgattacatc gacgagatgc tgacgatgat
     7981 cggccagctc atcaccaacg gctacgccta cgtcgccagc gacggcgacg tgatgtatgc
     8041 cgtgcacaag ttcgcgccgt acggaaaatt gtcgggcaag aagctcgaag acctgcgcgc
     8101 cggttcgcga gtcgaagtgg ataccaccaa gcgcgatcca ctcgatttcg tgttgtggaa
     8161 atcggcgaaa cccggtgagc cggcgtggcc ctcgccctgg ggtgacggcc ggcccggctg
     8221 gcacattgag tgctcggcca tgtccacccg ggagctcgga gatcatttcg acatccacgg
     8281 cggcggactc gatctcaagt ttccgcacca cgaaaacgag atcgcgcaga cctgcggggc
     8341 gacccacggc cgctttgccg aaatctggat gcacaacggc tttctcaaca tcgacaacga
     8401 aaagatgtcg aagtcgctcg gcaatttctt cacgacgcgc gaagtgctgg cgaagatcaa
     8461 gcatcccgag gtgctgcggt tcttcctgct gtcgagccac taccgcgggc cgatgaacta
     8521 ctcgccggat cagctcgacc aggccgaggc ggggttgaac cgtctgtaca ccgcgctgcg
     8581 cgacgtgccc gaagtcggcg gcgatctgta ttcgccgacg gccgccacgc aggt
//
LOCUS       DASQJS010000428         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2166413, whole genome shotgun sequence.
ACCESSION   DASQJS010000428 DASQJS010000000
VERSION     DASQJS010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166413"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..>2112
                     /locus_tag="VEW08_16870"
     CDS             56..>2112
                     /locus_tag="VEW08_16870"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HYJ42466.1"
                     /translation="MNSVVKIEHKDVDAIPFQEASLDIWDKKYRLSAKDGTPVDKTMD
                     DTYKRVARALADVEHESVREQWYERFVWALRRGAIPAGRVTSNAGALEHKPATSTINC
                     TVSGTIADSMDDILNKVHEAGLTLKAGCGIGYEFSTLRPRGAYVSGAGAYTSGPLSFM
                     DIYDKMCFTVSSAGGRRGAQMGTFDVGHPDAMEFIRAKRENGRLRQFNLSLLVTDDFM
                     KAVRADTDWNLAFPLLIKEYDPAEHKLDDATKFLWREWATTKGYVTREDGLVCCKIYK
                     TLPARRMWDVIMTSTYDFAEPGFILIDRVNEMNNNWWCENIRATNPCVPADTWVQTQD
                     GPRQVADLIGKPFTARVDGVDHASSPEGFFRTGVKPLVTMTTSEGHSLRLTSDHRVRR
                     ISTLTRFRLVTEWCAAGDLNAGDRVLLQDHRKAASWAGASETNMGTNEGYLMGLLVGD
                     GTVAEDRAELSIWARSAVGNSDIDVGGPHSVMAAALDAARCFNHRADFSGWHPGTGRG
                     EFRLSMGAITELSREVGLRPGRRTITPAIERASSEFYRGFLRGLFDTNGSVQGNQMKG
                     VSVRLANSDLVLLEGAQRMLLRLGIAASMYERAQHELVITGENVARFAEVVGFADTVK
                     RARLSRLLGGYKRTLNRERFVATVESVKPDGMEDVYDVQIPGINEFDANGFHAHNCGE
                     QPLP"
BASE COUNT          389 a          690 c          674 g          359 t
ORIGIN      
        1 ttccggttcg ggtgaatgtg cacaggccgg aacctcgggg agttgaaccc aatccatgaa
       61 tagcgtggtc aagatcgaac acaaggacgt cgatgcgatt cctttccagg aagcgtcgct
      121 cgacatctgg gacaagaaat atcgcttgtc cgccaaagac ggcactcccg tcgacaagac
      181 catggacgac acctacaagc gcgtcgcacg cgcgctggcg gatgtcgaac atgaatccgt
      241 tcgcgagcag tggtacgagc gtttcgtctg ggcgctgcgc cgtggcgcca tcccggcggg
      301 ccgcgtcacg tcgaatgctg gggcgctcga gcacaagccg gcgacgtcca ccatcaattg
      361 cacggtgtcc ggaaccatcg ccgattcgat ggacgacatc ctgaacaagg tgcacgaggc
      421 cggcctcacg ctgaaggcgg gttgcggcat cggttacgaa ttctccacgc tgcgtccgcg
      481 cggcgcgtac gtctcgggcg ccggtgcgta caccagcggc ccgctgtcgt tcatggatat
      541 ctacgacaag atgtgtttca ccgtctcgtc ggccggcggc cgccgcggcg cgcaaatggg
      601 cacgttcgac gtcggtcacc cggacgccat ggaattcatc cgcgccaagc gcgagaacgg
      661 tcgcctgcgc cagttcaacc tgtcgctgct ggtcaccgac gatttcatga aggcggttcg
      721 cgccgacacc gactggaatc tggcctttcc gctgctcatc aaagagtacg accccgccga
      781 acacaagctc gacgacgcga cgaaattcct gtggcgcgag tgggcgacca ccaagggcta
      841 cgtcacgcgc gaagacggtc tcgtctgctg caagatctac aagacgctgc cggcgcgccg
      901 catgtgggac gtcatcatga cgtccacgta cgatttcgcg gagccgggct tcatcctcat
      961 cgaccgcgtc aacgagatga acaacaactg gtggtgtgaa aacattcgcg ccaccaaccc
     1021 ctgcgtaccc gcagacacct gggtacagac ccaggacggc ccgcgccagg tggccgacct
     1081 catcggcaag ccgttcacgg cgcgcgtcga cggcgtcgat cacgccagct cgccggaggg
     1141 cttcttccgc acgggcgtga agccactggt gaccatgacc acgtccgaag ggcattcgct
     1201 gcgcctcact tccgatcacc gcgtgcgccg catctccaca ctgacgcgtt tccgcctggt
     1261 gacggagtgg tgcgccgcgg gcgacctcaa cgcgggcgat cgcgtgctgc tgcaagatca
     1321 tcgcaaggcg gcatcgtggg ccggtgcttc agagacgaac atgggcacca acgaaggcta
     1381 cctcatgggc ctgctggttg gcgacggcac tgtggccgaa gaccgcgcgg agctctcgat
     1441 ctgggcacgt tctgcggtcg gcaacagcga tatcgacgtc ggcggtccgc attcggtgat
     1501 ggcggcggcg ctcgatgccg cgcgctgctt caaccatcgc gctgatttct ccggctggca
     1561 cccgggcacg ggccgcggcg agttccggct gagcatgggt gcgattaccg agctgtcccg
     1621 cgaagtcggc ctgcggcccg gccgcaggac catcacgccc gccatcgaac gcgcttcgag
     1681 cgagttctat cgtggcttcc tgcgcggtct gttcgacacg aacggcagcg tgcagggcaa
     1741 ccagatgaag ggcgtctcgg tgcgcctcgc aaactcggac ctggtcctgc tcgaaggtgc
     1801 gcagcgcatg ctgctgcgtc tcggcatcgc cgccagcatg tacgagcgcg cgcagcacga
     1861 actggtcatc accggtgaga acgtcgcccg tttcgcggaa gtcgtggggt tcgccgacac
     1921 ggtcaagcgc gcgcgcctgt cgcgcctgct gggcggctac aagcgcacgc tgaatcgtga
     1981 gcgcttcgtg gccactgtcg agagcgtgaa gcctgacggc atggaagatg tgtacgacgt
     2041 gcagatcccc ggcatcaacg agttcgatgc caatggtttc cacgcgcaca actgcggcga
     2101 acagccgctg cc
//
LOCUS       DASQJS010000429         6138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1536478, whole genome shotgun sequence.
ACCESSION   DASQJS010000429 DASQJS010000000
VERSION     DASQJS010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536478"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VEW08_16875"
     CDS             complement(<1..220)
                     /locus_tag="VEW08_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HYJ42467.1"
                     /translation="MDGNGRWAEARGLARHAGHKEGVRPVRLCIEECARRGVGALTLF
                     AFSSENWQRPTVEVTSLMQLFLDAIDREV"
     gene            complement(283..840)
                     /gene="frr"
                     /locus_tag="VEW08_16880"
     CDS             complement(283..840)
                     /gene="frr"
                     /locus_tag="VEW08_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018679060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HYJ42468.1"
                     /translation="MLDDIKKDAATRMGKCVENFQGDLKKLRTGRAHPSLIENMKVDY
                     YGTDTPLKQVANISVEDARTLVVSPWEKSMVQAIEKAIHKSDLGLNPMSAGTVIRIPL
                     PPLTEERRKDITKVARADAENARVAVRNVRRDVITDVKEALKEKLISQDEEKKAEVDI
                     QKLTDKYIADIETQLAAKEKELLQV"
     gene            complement(866..1585)
                     /gene="pyrH"
                     /locus_tag="VEW08_16885"
     CDS             complement(866..1585)
                     /gene="pyrH"
                     /locus_tag="VEW08_16885"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HYJ42469.1"
                     /translation="MTAAPYKRILVKLSGEALLGTTEFGIDPAVLKRIASEVLEIQKM
                     GVQVACVIGGGNIFRGTGLARAGIDRATGDHMGMLATVMNALAMQDAIESLGGTARVQ
                     SAIRINQVCEDYIRRRAMRHLEKGRIVIFAAGTGNPYFTTDSAAALRAVEIEADILLK
                     ATKVNGIYDSDPMKNPDAKRYATLTFDKVLQDRLNVMDATAIVMCRDNGMPLQVFNLN
                     NAGDLPRVVRGDDIGTVVTND"
     gene            complement(1698..2612)
                     /gene="tsf"
                     /locus_tag="VEW08_16890"
     CDS             complement(1698..2612)
                     /gene="tsf"
                     /locus_tag="VEW08_16890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HYJ42470.1"
                     /translation="MNIPAEAVKQLRERTGAGMMECKKALVEAKGDLDAAAENMRKSG
                     LAKADKKASRVAAEGVVVIEKSADGKTGVLVEVNSETDFVARGDDFRGFAAEVAKAAL
                     AANTSNVEVINGLKLASGETVDEKRRALIAKLGENLTVRRVVLVTAPTIVGSYVHTDN
                     KKAALVAVEGGDADLARDLAMQVVAASPRYLTPADAPAEHVAKEREIETAKALAEGKP
                     AEIVAKMIEGRLRKSLNEFALTGQPFVKDADITIEKLLKDAKASVKSFERVEVGAGIE
                     KKQGDFAAEVMEQVKAAQTKTDPPATKH"
     gene            complement(2941..3666)
                     /gene="rpsB"
                     /locus_tag="VEW08_16895"
                     /pseudo
     CDS             complement(2941..3666)
                     /gene="rpsB"
                     /locus_tag="VEW08_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            3913..4713
                     /gene="map"
                     /locus_tag="VEW08_16900"
     CDS             3913..4713
                     /gene="map"
                     /locus_tag="VEW08_16900"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005170579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HYJ42471.1"
                     /translation="MDSLVSRHITIKSPAEQEKMRVAGRLAAEVLDMIGAHVKPGVTT
                     EELDRLCFEHITEVQKSIPANVNYRGFPKTICSSVNHVVCHGIPNDKVLKSGDILNID
                     VTVIRDGFHGDTSRMYYVGKPAPPAQRLAETCWEAMWRGIEKVKPGAHLGDIGHAIQT
                     FVEDRNFSVVREYCGHGIGRVYHEDPQVLHYGEPGTGDELKAGMTFTIEPMVNAGKRH
                     VRLLGDGWTVVTKDHSLSAQWEHTCLVTETGVEVLTLGEGSKRPAASN"
     gene            4714..>6138
                     /locus_tag="VEW08_16905"
     CDS             4714..>6138
                     /locus_tag="VEW08_16905"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYJ42472.1"
                     /translation="MESLEEDIEATETTALRNAPWLRLAHLPERLAEAAFAPDAFRTE
                     LRQANDELVERFRADESVEILVQARAELIDTILRQVWRSQLPPGYETWSLAAVGGYGR
                     GELHPHSDVDILILVPAAPDEAGRGVVERVVTFLWDINLEVGHSVRTVAQCAEESAAD
                     VGVMTTLIEARLLAGNAALFGEMREAVSADRIWPVNAYFEAKVAEQQDRHLKANDTAY
                     NLEPNVKTGPGGLRDIQTIAWVAKRHFGASSLDELLTHGFLSFTELRKLKQAQSFLWK
                     VRFGLHVLTGRREDRLLFDHQIKLAQSFGYEDASYTLAVEQFMQRYYRNVMDVMLLNE
                     LLLQLFREAILTETAPPRPLSPRFQVRNDYLEAVNEELFARTPSTLLELFVVLQQNPD
                     VKGVRASTIRAVNKNLWLIDEEFRQNPRNHRLFLEILRAPAGVTHELRRMNTYGVLGR
                     YIPAFGRIVGRMQYDLFHAYTVDAH"
BASE COUNT         1039 a         2001 c         1868 g         1230 t
ORIGIN      
        1 cgacctcgcg atcgatggcg tcgaggaaca gctgcatcaa actagtcact tcgaccgtgg
       61 gccgctgcca gttctcactc gagaacgcga acagcgtgag cgcaccgaca ccgcgccgcg
      121 cgcattcttc gatacacagg cgcaccggac gcacgccttc cttgtgcccg gcatggcgcg
      181 caaggccccg tgcctcggcc catcgtccgt tgccgtccat aatgatcgcg atgtgttgcg
      241 gagtgctcac ggtcagcggc aacgtgcaga gcggacgccc gctcacacct gcaggagttc
      301 tttctccttg gcggcaagct gagtctcgat gtccgcgatg tatttgtcgg tcagcttctg
      361 gatgtcgacc tcggccttct tttcttcatc ctgcgagatc agcttttcct tgagcgcttc
      421 cttcacgtcc gtgatcacgt cgcgacgcac gttgcgcacg gcgacgcgcg cattttcggc
      481 gtcggctcgc gccaccttgg tgatgtcctt gcggcgctct tcggtgagcg gcggcagcgg
      541 aatgcggatt accgtgcctg cggacatggg attcagcccc aggtcggatt tgtggatcgc
      601 cttctcgatg gcctgcacca tggatttttc ccacggtgaa accaccagcg tgcgcgcgtc
      661 ctcgacgctg atattggcca cctgcttgag cggcgtatcg gtgccgtagt aatcgacttt
      721 catgttctcg atgagagacg gatgcgcgcg accggtgcgc agcttcttga ggtcgccctg
      781 gaaattctcc acgcacttgc ccatgcgggt ggctgcgtcc ttcttgatgt cgtctaacat
      841 gattccttgc cctcgaggtt caacgtcaat cgttggtgac gaccgtgccg atgtcgtcgc
      901 cgcgcaccac gcgcggcagg tcgcccgcat tgttcagatt gaaaacctgc agcggcatgc
      961 cgttgtcgcg gcacatcacg atggccgtgg cgtccatgac attgaggcga tcctgcagca
     1021 ccttgtcgaa ggtcaatgtg gcgtagcgct tcgcatccgg gtttttcatc ggatccgaat
     1081 cgtagatccc gttgaccttg gtggctttca acaggatgtc ggcttcgatc tcgacggcgc
     1141 gcaaggctgc ggcgctgtcg gtcgtgaaat aagggttgcc cgtgcccgcc gcgaaaatca
     1201 cgatgcggcc tttctcgagg tggcgcatcg cacggcggcg aatgtagtcc tcgcagacct
     1261 ggttgatccg gatcgcactc tgcacgcgcg ccgtgccacc caggctctcg atggcgtcct
     1321 gcatcgccag cgcgttcatg acggtcgcca gcatgcccat gtggtcgccc gtggcgcggt
     1381 cgatgccggc gcgggcgaga cccgtgccgc gaaagatgtt gccgccgccg atgacgcacg
     1441 ccacctgcac gcccatcttc tggatctcga gcacttcgct cgcgatgcgc ttgaggaccg
     1501 ccggatcgat gccgaactcg gtggtgccca gcagcgcctc tcccgaaagc ttgacgagaa
     1561 tgcgtttgta cggcgcggcg gtcatttgct cgattcccct tgtctctgcc tggagggcct
     1621 cgtttattga ggagccccgc ggcgttgcgc actttgccat aaagaaaacc ccgctagagg
     1681 cggggttttc gtatcggcta gtgcttggtc gccggagggt cggtcttggt ctgggcagct
     1741 ttgacctgtt ccatgacttc ggccgcgaag tccccctgct tcttttcgat gcccgctccg
     1801 acctcgaccc gctcgaagct cttcacgctg gctttggcgt ccttgagcag cttctcgatg
     1861 gtgatgtcgg cgtccttgac gaacggctga ccggtaagcg cgaactcgtt gagcgacttg
     1921 cgcagacggc cttcgatcat cttggcgacg atttcagccg gcttgccctc ggcgagcgcc
     1981 ttggcggttt cgatttcgcg ctctttcgcc acgtgttccg ccggcgcatc cgccggggtc
     2041 aggtagcgcg ggctcgccgc gaccacctgc atggcaaggt cgcgcgccag atccgcgtcg
     2101 ccgccttcga cggccacgag cgccgccttc ttgttgtcgg tgtgcacgta ggagccgacg
     2161 atggtcggtg ccgtcacgag caccacgcgg cgcacggtga gattttcacc aagcttggcg
     2221 atcagcgcgc gacgcttctc gtctaccgtt tcaccgctcg cgagcttcag gccattgatg
     2281 acctcgacgt tcgacgtgtt cgcggccagc gccgccttgg cgacttcggc cgcaaagcca
     2341 cggaagtcat cgccacgcgc gacgaaatcg gtttcgctgt tcacttcgac cagcacaccg
     2401 gtcttgccgt cagcggactt ctcgatgacc accacgcctt cggcggcgac tcgcgaggcc
     2461 ttcttgtcgg ccttggcgag acccgacttg cgcatgttct cagcggcggc atccagatcg
     2521 cctttcgctt cgacgagtgc cttcttgcac tccatcatgc cggcgccggt gcgctcgcgc
     2581 agctgtttga ccgcttctgc gggaatgttc attcgtccat ccttaaatta acgaggcctc
     2641 aaacttcgaa cggggcgatc agccgcgacg cggaccgccg ccaccgccgc gaccacgacc
     2701 cggggcattg cccgtgccgg tgcgcgaacg accggcgctg gccgtggcgc tgcgcggagc
     2761 cgcttcgccc gtgtccgtga acacggccgg atcggcaccg tcttccggat cggcagcaac
     2821 gcttgccacc acgggacgac ggcgcgaggc agtgttggcc gggcgaccac ctcgacgctg
     2881 cggcggaggc gccttcgggg gtgccgcacg cgcgcccttc ttgcggggaa tgccattctc
     2941 gtcgagctcg acgaactcgt cttcgcccac ggcgacggcc ggagcctgcg aacgaccttc
     3001 gatgaccgaa tcggcgatgc cggaggcata tagagatatg gctcgcatgg cgtcatcgtt
     3061 ccccgggatc acgtaatcca cgttgtcagg cgcgcagttg gtatccacca ccgcgaccac
     3121 cgggatgccg agcttcttgg cttcgaggat ggcgatttcc tcgtggccga cgtcgatgat
     3181 gaagatgacg tccgggggcg actccatctt cttgatgccg cccaggctct tctcgagctt
     3241 gtccttctcg cggcgcatca tcgtggcttc tttcttgccg cgcttgccga gcgtgccgtt
     3301 ttccgacatc tcggtgagat ccgccagccg cttgatggag ccgcggatgg tcttgaaatt
     3361 ggtgagcatg ccgcccagcc agcgctggtt gacgaagggc atctcgcaac ggcgagcttc
     3421 cttctctacg gcttcgcgag cggaacgctt cgttccgacg aacagcacct tgccgccgtc
     3481 cgcgaccaca ccacgcacga aagccgccgc ttccgaatac atcggctgcg tcttttcgag
     3541 gttgatgatg tggatcttgt ttcgttcgcc gaaaatgaac ggagccatct tcgggttcca
     3601 gaagcgggtc tggtgtccaa aatggacgcc cgcttccagc atttgtcgca ttgacacgcc
     3661 ggacatcaaa tttctcctgg tgggttaagc ctccgcgccg cccgggtgga tatccttaga
     3721 ttgctctcgg gacacccatc cacccgttat gcagaccgcc tggagaggcg aatcttttta
     3781 aagaccggct tttcttcgaa aagcctgggc tgcaggttgc gcgtgtggtt taagtgagtt
     3841 tcattgccaa aaaaggccgc gctttatacc atcccttccc cgtcaaaaca atgccttacg
     3901 caggtgcacc ccatggactc cctcgtgagc cgccacatca ccattaagtc cccggccgag
     3961 caggaaaaaa tgcgggtcgc agggcggctg gcggccgaag ttctggacat gatcggggcg
     4021 cacgtcaaac ccggcgtgac caccgaggag ctcgatcgcc tgtgttttga gcacattacg
     4081 gaggtccaga agtcgattcc cgcgaacgtg aactaccgag gctttccgaa gaccatctgc
     4141 tcttcggtga accacgtggt gtgccatggc atcccgaacg acaaggtgct caagtccggg
     4201 gacatcctga acatcgacgt cacggtgatt cgcgatgggt tccacggtga caccagccgg
     4261 atgtactacg tgggaaaacc cgccccgccc gcgcagcgcc tcgccgaaac ctgctgggaa
     4321 gccatgtggc gcggcatcga aaaggtcaaa cccggtgcac acctcggcga catcggccac
     4381 gccatccaga ctttcgtgga agaccgcaat ttctccgtgg tgcgcgaata ttgcggccac
     4441 ggcatcggtc gcgtgtacca cgaagatccg caggtcctgc attacggcga gcccggcacg
     4501 ggcgatgagc tcaaggccgg catgaccttc acgatcgaac ccatggtgaa cgccggcaag
     4561 cggcacgtgc ggttgttagg cgatggctgg accgtggtca ccaaggacca ctcgctgtca
     4621 gcgcagtggg aacacacgtg cctggtcacc gagaccggcg tcgaagtgct gacgctcggc
     4681 gagggcagca agcgccctgc ggcatcgaac tagatggagt cgctggagga agatatcgag
     4741 gcaaccgaaa cgacggccct gcgcaacgct ccctggttgc gcctcgcgca tcttccggag
     4801 cggctcgccg aggcggcatt cgcgcccgac gcgtttcgca ccgagctgcg ccaggccaac
     4861 gacgaactgg tggaacggtt tcgcgccgac gaatcggtcg aaatcctggt gcaggctcgc
     4921 gccgagctca tcgacaccat cctgcggcag gtgtggcgtt cgcagttgcc gcccggctat
     4981 gagacctggt cgctggccgc ggtgggcggt tacggtcgcg gcgagctgca tccacattcc
     5041 gacgtcgaca tcctgatcct ggttccggcc gcgccggacg aagccggccg tggcgtggtc
     5101 gaacgcgtgg tcacgttcct ctgggacatc aatctcgagg tgggccacag cgtgcgcacc
     5161 gtggcccaat gcgccgagga aagcgccgcc gatgtcggcg tcatgaccac gctcatcgag
     5221 gcgcgcctgc tggccggcaa tgcggccttg tttggtgaaa tgcgcgaggc cgtgtccgcc
     5281 gatcgcatct ggcccgtgaa tgcctacttt gaagccaagg tcgccgagca gcaggaccgt
     5341 cacctcaagg ccaacgacac cgcctacaac ctcgagccga atgtgaagac cggtccgggc
     5401 ggtttgcgcg acatccagac cattgcctgg gtggccaagc gccacttcgg cgcgagctca
     5461 ctggacgaac tgctcaccca cggtttcctg tccttcaccg agctgcgcaa gctcaaacag
     5521 gcgcagtcct tcctctggaa ggtgcgcttc gggctgcacg tgctgaccgg caggcgcgaa
     5581 gaccgcctgc tgttcgatca ccagatcaaa ctggcgcaga gcttcggcta cgaagacgcc
     5641 tcgtacacgc tcgccgtcga gcagttcatg cagcgctatt accgcaacgt gatggacgtg
     5701 atgctgctca acgaactgct gctgcaattg ttccgcgagg ccatcctcac cgaaaccgcg
     5761 ccgccacgtc cgctttcccc gcgcttccag gtacgcaacg actatctgga agcggtgaac
     5821 gaggagctgt tcgcgcgcac gccgtccacg ctgctcgagc tgttcgtggt gctgcagcag
     5881 aacccggatg tgaaaggcgt gcgcgcctcg accatccgcg ccgtgaacaa gaacctgtgg
     5941 ctgatcgacg aagagttccg ccagaacccg cgcaaccatc gtctgttcct cgagatcctg
     6001 cgggccccgg cgggcgtgac ccatgagttg cgccgcatga atacctacgg cgtgctcggc
     6061 cggtacattc ccgcgttcgg ccgcatcgtc gggcgcatgc agtacgacct gttccacgcc
     6121 tacaccgtcg atgcgcac
//
LOCUS       DASQJS010000430        10283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3734754, whole genome shotgun sequence.
ACCESSION   DASQJS010000430 DASQJS010000000
VERSION     DASQJS010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10283
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3734754"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VEW08_16910"
     CDS             complement(<1..851)
                     /locus_tag="VEW08_16910"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYJ42473.1"
                     /translation="MNAGAGARDERGAFNLSAWALTHKPLIGFLMVIALLAGLRAYNN
                     LGRDEDPPFTIKTMIVRAMWPGADAIQTTKQLTDRIEKPLESLEFIDFVTSFTKPGEA
                     TIMVNLRDNTPPEAVPGQWYQVRKKIGDIRGTLPQGSIGPFFNDDFGDVYGVIYALTS
                     DGFTYRELRDQAEFIRAELLRVNNVGKVDLIGVQDEVIYIDFSLRQMAGLGIDPDLVA
                     LTLASQNIVIASGTIETPQERIAVRVSGALDSVTSLENIAIRVGDRRVRLKDFAHVTR
                     GYKDPPSP"
     gene            complement(848..1942)
                     /locus_tag="VEW08_16915"
     CDS             complement(848..1942)
                     /locus_tag="VEW08_16915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYJ42474.1"
                     /translation="MSFDNKYPVRGSLLLIALAAAIAGCGGKEEPETLVRPVRSVIVT
                     GGASADSATYTAEIRSRYETDLSFQVAGKLVNRAVDAGAAVKKGQLLAQLDQTDQQLG
                     VDAARGAVPAAQSDLDRSRSEAARFRDLLERGLTTRAQYEAQQTAVQSAEAKLSQATA
                     ELRLAQQRLGYTTLRAGEDGVVTRVMVDVGTVVSAGQRALSIARPSELEAVFDVPDGR
                     IDEVKAGASVLINPLSGDTAAWAGSVREISPSADPVTRTYQVRSSIVKPPPSLRLGMT
                     VSVTLPRVGGAPNISLPSTAIFQKDGKPAVWIVTTASIVELRPVVVERYDTDRVYVAD
                     GIRTGERVVTAGVHRLAPGEKVKLAAEKPQ"
     gene            complement(1951..4293)
                     /locus_tag="VEW08_16920"
     CDS             complement(1951..4293)
                     /locus_tag="VEW08_16920"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HYJ42475.1"
                     /translation="MSFRFGQALSALALSLLTWSAQARDIRVAVVTDGPTVRQLLTAE
                     VIEREIANVAGSGLKVVLPPDKRFAGDWSLDGAAAVLDRALADRDVDVVVTLGILTSQ
                     QAARRASLPKPVIAPLVIDPVLQSYPLIEGRSGRHNFTYVADFQSVATNVRAFHDIVG
                     FKHMVALVENSLLAALPALSGKATELAATLNIRIDIVRVGDDVSAVLASLPPGADAVY
                     VTPLRFNEGQARELARGLAQRKLPTFSVMGRGDVEGGLLLTTGGAERDNERLARRLAI
                     MIQRIAQGEDPAQFDVAFPTSQRLVINMQVAREIGFSPRWQFLADAEQLHAEPDGAQP
                     LTLLGAMRAALDANPSLAASRERLGSALDDVRIARGELLPSLSASAARTRIDDDRASP
                     LTQAEDTTSAGLSFSQIIYSERAWAGYSIARSLGAAQEQSQRADMLNTLTDAAAAYLN
                     VLRAKSVEEVRRRNVENTRKNLEISRVREEVGLAGRSDYLRWVAQLARDKQTLLAAES
                     GRRQAETDMLRILHRPASQPFSTVESGIDDPLTLVSSPRTQAFLDTPAKWAVFMEYAV
                     HTALENAPEIKQASSVLEARQRALTSAGRAYYLPDLALVSNGSKFTDKSGAGSLNVPG
                     APDDESWSVSLQATLPLVTGGQRGAERSQARHEMRASEADRLATMDGVEARTRLVLHQ
                     TASSWPAIDLSREAQAAADENLANVTDAYARGAVSVTDLIDAQETALLNGLSATDAKY
                     GFMTDFINVLRSMGDFEILLDPASREAWFVRVETWFREHP"
     gene            4418..5236
                     /locus_tag="VEW08_16925"
     CDS             4418..5236
                     /locus_tag="VEW08_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42476.1"
                     /translation="MVNDSIPGEQTGQLEALLERWQTSLDLHARYAALDEARYWHVQP
                     WPKHERPQRWIIQLARKRILALKRIVAQRQAEGDRAFIEGIEIMSFLATLVGLTSVER
                     FIPLATHETERRDVLSAKPEPAKASPAPVAARSAPTADTTRSRTPAASRTGEHRRTAE
                     TTRQMPVLAQPRREAKRDPGRPATIKPQPAARPQPPVRSPPSVKPRKAPSTVTAPEHV
                     VIADAVRLLGWGKQWHELADMIARLSERPSPSEIRRILRTYRDQIDAVATRRRD"
     gene            5369..6079
                     /locus_tag="VEW08_16930"
     CDS             5369..6079
                     /locus_tag="VEW08_16930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ42477.1"
                     /translation="MIKTQNLTKSYDGLLAVDDLTFSVEPGEVLGFLGPNGAGKSTTM
                     RMLAGFITPTSGTGSICGHDVATDALAAKAVLGYLPEGAPNYGEMSVRSFLEFIADLR
                     RLEGAHRKSRMDYVIGRLELEPVITQTIETLSKGFKRRVGLAQAIIHDPKVLILDEPT
                     DGLDPLQKHQVRTLIGEIAREKTIVISTHILEEVDAVCTRAIIVAKGKLVADDTPAGL
                     KTRAPSGRLEDVFRSVAA"
     gene            6102..6836
                     /locus_tag="VEW08_16935"
     CDS             6102..6836
                     /locus_tag="VEW08_16935"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ42478.1"
                     /translation="MKTVAIIMRREFASYFATPIAYVVLVIFLALANLFGFSFGSLYE
                     RGQADLTPFFKFLPWLYLFLIPAVSMRLWAEERRNGTIELLLTQPVTLWQAVLGKFFA
                     AWVFVGIALALTFPVWITINYLGNPDNGAILAAYLGSLFFAGGFLAVGSFTSSITKNQ
                     LVAFLIALLMCFLLLVAGYPVVTDFLPKWAPQWLVDGIASLSFDTHFENIIKGVLDLR
                     DVLYFVLVTIFFLLASTVVLDARKSK"
     gene            6853..8760
                     /locus_tag="VEW08_16940"
     CDS             6853..8760
                     /locus_tag="VEW08_16940"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HYJ42479.1"
                     /translation="MFKKSHLGATGLAVVGVLFIAIMLLANLLLRGAKFDLTADNLYT
                     ISDGTDHIVHGLKEPVNLYLFFSEKTATPIPQIRNHGIRVRELLEELVSRSNGKLTLK
                     VIDPQPFSEEEDRAQELGVTSMPIGATGDKLYLGLAATNSTDGKEAIAFLDPQSEEQL
                     EYDVAKLIHKLSSAKKPVIGWLSSVPMQGDFDMQTGRPRQPWVVYGQVEQLYTVRNLE
                     PSLTTIGSDVDVLVIVHPKELPPAALYAIDQFALRGGHILAFVDPNAQMDTAGADPNN
                     PMAAMTADKSSHLEPLLTAWGLEFKSDQVVADLERGLSVSMREGEAPSQHIAILGFDH
                     GSMSKDVVTARLDSINMATAGSLKPLSSSKEGGTKVKVEPLITTSAQAGLIPAQRFAM
                     MSDPASLRDGFKPTGEIVVAARVSGNGVSAFPDGPPPGAAAAPDALKASAKPLNIVVI
                     ADSDLLSDFMWVQQRNFFGQTIAQPFANNGELVWNALDNLSGSADLISIRGRAPYSRP
                     FERVEELRRAADARFRSTEQQLETQLQQTEEQLNKLQSAQPGGNEAILSPEAANAIER
                     FQDEKLRIRKELRKTKADLELDIKALGTRLKLINVLTMPLTIIGIGLLVYFWRKRRRH
                     AIAMLRKGSAA"
     gene            8757..9854
                     /locus_tag="VEW08_16945"
     CDS             8757..9854
                     /locus_tag="VEW08_16945"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HYJ42480.1"
                     /translation="MNQKKFLALTVTTVVVLAAGVWISMHRSSKQSDLGGTAMFADLK
                     PALGEVSEVRFAKGDGSRTTLRKSADGWTVVERQYPADASRVRELVLNLASLKVVERK
                     TQDPANYARLGVEAPDSPTAGSTLVEVIADKKTWSLIVGKGADGKAIYVRKPDETVSL
                     LAAPFITVDPDQKRWIDRLLVDVPGANVHDVSVKLANSPAYVLTRAARGAQDLSVSPV
                     PKGRKAATSMSLGSQTEALAAFHFDDVRTAPSAAPGASDTATYRMFDGQVIEFTGHRE
                     GDKAFVTVATRRDPALAAKFPEAPPPPAPPSTPAAPATAPAATPGADQIAEKLGARAK
                     GMEFEIPAYKYEAIFRKPEELLEKPAESVKD"
BASE COUNT         1820 a         3470 c         3195 g         1798 t
ORIGIN      
        1 ggcgaaggcg gatccttgta gccgcgcgtg acatgcgcga aatccttgag gcgcacgcgc
       61 cggtcgccca cgcgaatggc gatgttctcg agcgaggtca ccgaatcgag cgcgccgctc
      121 acgcgcaccg cgatgcgttc ctgcggcgtc tcgatggtgc ccgaggcgat cacgatgttc
      181 tggctggcca gcgtcaaggc caccaggtcc ggatcgatgc cgagtcccgc catctgccgc
      241 aggctgaagt cgatgtagat gacttcgtcc tgcacgccga tgagatccac cttgcccacg
      301 ttgttcacgc gcagcagttc ggcgcgaatg aattcggcct ggtcacgcag ctcccgatac
      361 gtgaagccat cgctggtcag cgcgtagatc acgccgtaca catcaccgaa atcgtcgttg
      421 aaaaatggac cgatggaacc ttgcggcagc gttcccctga tgtcgcctat cttcttgcgc
      481 acctggtacc actggccggg cacggcctct ggcggcgtgt tgtcgcgcag gttgaccatg
      541 atcgtggctt cgccgggctt cgtgaagctg gtgacgaagt cgatgaattc gagcgactcg
      601 agcggcttct cgatccggtc cgtgagctgc ttcgtcgtct ggatggcatc ggcgccgggc
      661 cacatcgcgc gcactatcat ggtcttgatg gtgaacggcg gatcctcgtc acggccgaga
      721 ttgttgtagg cgcgcagacc ggcgagcagc gcgatcacca tgagaaaccc gatgagcggc
      781 ttgtgggtca gcgcccacgc ggagagattg aaggcgccac gctcgtcgcg cgcgcccgca
      841 cctgcattca ttgcggcttc tcggccgcca gtttcacttt ctcgccgggc gcgagccgat
      901 gaactccggc agtgaccacg cgctcaccgg tgcgtatgcc gtcggcgacg tacacgcggt
      961 cggtgtcgta tcgctcgacc accaccggac gcaattccac gatgctggcc gtcgtgacga
     1021 tccacaccgc cggcttgccg tccttctgga agatggcggt ggatggcagc gagatattgg
     1081 gtgcaccgcc gacgcgcggc agggtcacgc tcacggtcat gccgagacgc aacgatggcg
     1141 gcggtttcac gatggagctc cgcacctggt aggtgcgcgt caccgggtcg gcgctcgggc
     1201 tgatctcgcg cacgctgccg gcccatgcgg cagtgtcgcc actcagggga tttatcaaca
     1261 cggacgcgcc cgctttcact tcgtcgattc gtccgtcggg cacgtcgaac acggcctcga
     1321 gttcgcttgg acgcgcaatg ctcagcgcgc gctggcccgc ggataccacg gtgcccacat
     1381 cgaccatgac gcgcgtgacc acgccatctt ctccggcgcg cagcgtcgtg tatcccaggc
     1441 gttgctgggc cagccgcagt tccgccgtgg cctgcgatag tttggcctcc gcggactgca
     1501 ccgcggtctg ctgcgcctcg tattgcgcgc gcgtcgtcag gccgcgttcc agcaggtcgc
     1561 gaaaccgcgc cgcttcgctg cgcgagcggt cgaggtccga ttgcgcggcc ggcaccgcgc
     1621 cgcgcgccgc gtccacccct aactgctgat cggtctggtc gagttgcgcg agcaactggc
     1681 ctttcttcac ggccgcgccg gcatcgacgg cacggttcac gagcttgccg gccacctgga
     1741 aactcaggtc cgtctcgtag cgagaacgga tctccgcggt gtacgtcgcg ctgtcggcgg
     1801 acgccccgcc ggtgacgatc accgagcgca cgggacggac cagtgtttcg ggctcctctt
     1861 tcccgccgca gccagcgata gctgccgcca gcgctattaa taagagagac ccccggacgg
     1921 ggtacttatt gtcgaatgac attccgagcc tcatggatgt tcccggaacc aggtttccac
     1981 gcgcacgaac cacgcctcgc gggatgcggg gtcgagcagg atttcgaagt cgcccatcga
     2041 gcgcagcaca ttgatgaaat cggtcatgaa tccatatttc gcgtcggtgg cggaaagacc
     2101 gttgagaagc gccgtctcct gtgcgtcgat gaggtcggtg accgagactg cgccgcgcgc
     2161 gtacgcgtcg gtgacattgg cgaggttctc atcggcggcc gcctgcgcct cgcgcgacaa
     2221 gtcgatggcc ggccatgagc ttgcagtctg gtgcaacacc agtcgcgttc tcgcttcgac
     2281 gccgtccatg gtcgccagcc ggtcggcttc gctggcccgc atctcatggc gcgcctgaga
     2341 gcgctcggcg ccgcgttgtc cgccggtgac cagcggcaat gtggcctgca aagacacgct
     2401 ccaggattca tcgtcgggag cgccgggaac attcagcgaa cccgcaccgc tcttgtcggt
     2461 gaatttggag ccgttcgaca ccagcgccag gtcgggtaga tagtacgcgc gcccagccga
     2521 ggtcaacgcg cgctgccgcg cctcgagcac ggagctcgcc tgcttgatct ccggcgcgtt
     2581 ctcgagcgcc gtgtgcacgg cgtattccat gaacaccgcc cacttggcag gcgtatcgag
     2641 aaacgcctgg gtacgcgggc tcgacaccag cgtcagcgga tcatcgatgc ccgactccac
     2701 cgtgctgaaa ggctggctcg ccggacgatg caggatgcgc aacatgtcgg tttccgcctg
     2761 ccgccggcct gattccgccg ccagcagcgt ctgtttgtcg cgcgcgagct gcgccaccca
     2821 ccgcaggtag tcggatcgcc cggccaggcc gacctcttcg cgcacgcgcg agatctcgag
     2881 attcttccgc gtgttctcga cgttgcgccg gcggacttcc tcgaccgatt tggctcgcaa
     2941 cacgttgagg taggccgcag ccgcgtcggt aagcgtattc agcatatcgg cgcgctggct
     3001 ttgttcctgc gccgcgccca gagacctggc aatggagtag ccggcccagg ctcgctcgga
     3061 gtagatgatc tggctgaacg aaagtcccgc actggtcgtg tcttcggcct gggtaagcgg
     3121 gctggcgcgg tcgtcatcga tgcgcgtgcg cgcggccgac gccgataacg atggcaacag
     3181 ttccccacgt gctatccgca catcgtcgag cgcactgccc aggcgttcac ggcttgctgc
     3241 cagcgaaggg ttggcatcga gcgcggcgcg catcgccccg agcagcgtga gtggctgcgc
     3301 accatcgggc tccgcgtgca actgctctgc gtccgccagg aattgccagc gcggcgagaa
     3361 accgatctcg cgcgccacct gcatgttgat gaccagccgc tgcgaggtgg ggaacgccac
     3421 gtcgaactgc gccggatcct caccctgcgc aatgcgctgg atcatgatgg ccaggcgccg
     3481 ggccagccgc tcattatcgc gctcggcgcc gcccgtggtc agcagcagcc cgccttcgac
     3541 gtcaccgcgg cccatgactg aaaaggtagg tagcttgcgt tgcgcgaggc cgcgcgcgag
     3601 ctcgcgcgcc tggccttcgt tgaatcgcag cggcgtcacg tacaccgcat cggctccggg
     3661 cggaaggctg gcgagcacgg cgctgacgtc atcgcccacg cgcacgatgt cgatacgtat
     3721 gttgagcgtg gccgcaagct cggtggcctt gcccgacagg gccggcaacg cggccagcag
     3781 cgagttctcc acgagcgcca ccatgtgctt gaagccgacg atgtcgtgaa acgcacgcac
     3841 gttggtggcg acgctctgaa aatcggccac gtacgtgaaa ttgtgccgtc cgctgcggcc
     3901 ctcgatcagt ggataggact gcaggaccgg gtcgatgacc agcggcgcga tgacgggctt
     3961 gggcagggac gcgcgccgcg ccgcctgttg tgaggtgagt atgccgagcg tcaccaccac
     4021 gtccacgtcc cgatcggcga gcgcgcggtc gaggaccgcc gccgcaccgt cgagcgacca
     4081 gtccccggcg aaacgcttgt cgggtggcag cacgaccttg agtcctgacc cggcgacatt
     4141 ggcgatttcg cgttcgatga cctccgcggt gagcagctgc cgcaccgtgg gcccgtcggt
     4201 aaccacggcg acacgaatat cgcgcgcctg cgcggaccat gtgagcaagg acaatgccaa
     4261 cgcactgagc gcctggccga agcgaaagct catccgcgac ttctccccgt ccggctcttt
     4321 gtttgcgtgc atcttagtcc gcgtccaact gtgctgtcat gcacaaatgt cgccggcgcg
     4381 cgcaccggcg acacgcatcg gcgctactct tcggtgcatg gtcaacgaca gtatccccgg
     4441 cgaacagact ggccagctcg aagcgttgct cgagcgctgg cagaccagtc tcgacctgca
     4501 cgcacgatac gcagcgctcg atgaggcgcg ctactggcac gtgcagccat ggcccaagca
     4561 cgaacgcccg cagcgctgga tcatccagct ggcacgcaag cgcatcctcg cactcaaacg
     4621 catcgtcgcg cagcgccagg ccgaaggtga tcgcgccttc atcgaaggca tcgagatcat
     4681 gagttttctc gccacgctgg tgggactcac atccgtggag cgattcattc cgctggccac
     4741 gcacgaaacc gaaagacgtg acgtgctgag cgcgaagccg gaacccgcga aggcgtcgcc
     4801 tgcacccgtg gccgccagga gtgcgccaac ggccgacacc acccgttcgc gtacacccgc
     4861 cgcatcgcgt accggcgaac accgccggac tgccgaaacc actcgccaga tgccagtgct
     4921 ggcgcagccc aggcgtgagg cgaagcgcga tccgggtcgt ccggcgacca tcaagcccca
     4981 gcctgccgca agaccccagc ctcccgtcag atcccctccg tccgtcaaac ccaggaaagc
     5041 cccgtcgacc gtcacggcgc ccgaacacgt ggtgatcgcc gacgccgtac gcttgttagg
     5101 ctggggcaag cagtggcatg agctcgcgga catgatcgcg agactctccg agcggcccag
     5161 cccctcggaa atccgccgca tcctgcgcac ctaccgcgac cagatcgacg ccgtcgccac
     5221 ccgccgacgt gactagcgca gggattacaa gcgcgtaatc tgccgcgccc ggggcggcgt
     5281 tgtcttgcgc gaaggcgcgc tcctcagcag aatctgcgcc ccggaaccca cggaactatc
     5341 cgcacggcca ccgctctcaa caggcgccat gatcaagacg cagaacctga ccaaaagcta
     5401 cgacggcctg ttggccgtcg atgacctcac tttcagcgtg gaaccgggcg aagtcctggg
     5461 attcctcgga cccaacggag ccggcaagtc caccaccatg cgcatgctgg cgggcttcat
     5521 cacacccacc agcggcaccg gcagcatttg cgggcacgac gtcgccaccg atgcgctggc
     5581 cgccaaggcg gtgctgggct atctaccgga aggggcaccg aactacggtg aaatgtcggt
     5641 gcgcagcttc ctcgaattca tcgccgacct gcgccggctc gaaggcgccc accgcaaatc
     5701 gcgcatggac tacgtcatcg gccggctcga gctcgagccc gtcatcaccc agaccatcga
     5761 gacgctgtcc aagggcttca agcgcagggt cggactcgcc caggccatca ttcatgaccc
     5821 gaaggtgctg atcctcgacg agcccaccga cggcctcgat ccgctgcaga agcaccaggt
     5881 ccgtacgctc atcggcgaaa tcgcgcgtga gaagaccatc gtcatctcca cgcacatcct
     5941 cgaagaagtc gacgccgtgt gcacacgcgc catcatcgtg gcgaagggca aactcgtggc
     6001 cgacgacacg cccgccggac tcaagacgcg cgccccctcg ggccgtctcg aagacgtgtt
     6061 ccgctccgtc gccgcctgaa ccccactttc cggagccccc tttgaaaacc gtcgcaatca
     6121 tcatgcgccg cgagttcgcg agctatttcg ccactcccat cgcgtatgtc gtcctcgtca
     6181 tcttcctggc gctggcgaac ctgttcggct tttccttcgg ttcgttgtac gaacgcggcc
     6241 aggcggatct cacgccattc ttcaagttcc tgccgtggct ctatcttttc ctgatccccg
     6301 cggtgtcgat gcgcctgtgg gcggaggaac ggcgtaacgg caccatcgaa ctgctgctca
     6361 ctcagccggt taccctgtgg caggcggtgc tgggcaagtt cttcgcggcc tgggtgttcg
     6421 tcggaattgc gctcgcgctc actttcccgg tctggatcac catcaactac cttggcaatc
     6481 cggacaacgg cgccatcctc gccgcctacc tgggctcgct gttcttcgcg ggtggattcc
     6541 tggccgtggg ctcgttcacc tcgagcatca ccaagaatca gctcgtcgcc tttctcatcg
     6601 ccttgctgat gtgcttcctg ttgctggtgg ccgggtatcc cgtggtcacc gatttcctgc
     6661 cgaagtgggc accgcagtgg ctggtcgacg gcatcgcctc gctgtcgttc gacacgcact
     6721 tcgagaacat catcaagggc gtgctcgacc tgcgtgacgt gctgtacttc gtgctggtca
     6781 ccatcttttt cctgctcgcg agcaccgtgg tgctggacgc gcgcaagtcg aaataacctg
     6841 gtgtcaaaaa cgatgttcaa gaaatcccat ctcggcgcca ctggcctcgc ggtcgtcggc
     6901 gtgttgttca tcgccatcat gctgctggcc aacctgctgc tgcgtggcgc gaagttcgat
     6961 ctcaccgccg acaatctcta caccatctcc gacggcaccg accacatcgt gcacggcctc
     7021 aaggagccgg tgaacctcta cctgttcttc tccgagaaga cggccacgcc cataccgcag
     7081 attcgcaacc acggcatccg agtgcgcgaa ctgctcgaag agctggtgtc gcgttcgaat
     7141 ggcaaactca cgctcaaggt catcgacccc cagccgtttt ccgaggagga agaccgcgcc
     7201 caggagctcg gcgtcacttc gatgcccatc ggcgccaccg gcgacaaact ctatctcggc
     7261 ctggcggcga cgaattccac ggacggcaag gaagccattg cgttcctcga tccgcagtcc
     7321 gaggagcagc tggaatacga cgtcgccaag ctgatccaca agctctcgag cgcgaagaag
     7381 ccggtcatcg gctggttgtc atcggtgccc atgcagggcg acttcgacat gcagaccggc
     7441 cggccgcgcc agccctgggt tgtctacggc caggtcgagc agctctatac ggtgcgcaac
     7501 ctcgaaccgt cgctcaccac catcggcagc gacgtagacg tgctggtcat cgtgcacccg
     7561 aaagagctgc cgcccgcggc gctgtacgcc atcgaccagt tcgcactgcg cggcggacac
     7621 attctcgcct tcgtcgatcc gaatgcgcag atggatacgg ccggggccga ccctaacaac
     7681 ccgatggcgg cgatgaccgc cgacaagtcc tcgcatctcg aaccgctgct caccgcgtgg
     7741 ggcctggaat tcaagtccga ccaggtggtc gcggatctcg agcgcgggct ctcggtgtcg
     7801 atgcgcgagg gtgaggcgcc gtcgcagcac atcgccatcc tcggattcga ccacggcagc
     7861 atgtccaagg atgtggttac cgcgcggctc gattccatca acatggcgac ggcgggtagt
     7921 ttgaagcccc tgtcttcgtc gaaagagggc ggaaccaagg tcaaggtcga gcctctcatt
     7981 acgaccagcg cgcaggcggg actgataccg gcgcagcgct ttgccatgat gagtgacccg
     8041 gcttcgctgc gtgacggctt caagccgacc ggcgagatcg tggtcgccgc gcgcgtttcc
     8101 ggaaacgggg tcagcgcatt tcccgatggc ccaccgccgg gtgcggccgc ggcgcccgat
     8161 gcgctgaagg cgtccgcgaa gccgctgaac atcgtggtca ttgcggattc ggacctgctg
     8221 tccgacttca tgtgggtgca gcagcgcaat ttcttcggcc agaccatcgc gcagccgttc
     8281 gccaacaacg gcgagctggt gtggaacgcg ctcgacaatc tcagcggcag cgccgatctc
     8341 atcagtattc gcggacgcgc accctactcc cgaccgttcg agcgcgtcga agagctgcgt
     8401 cgcgccgccg acgcgcgctt ccgctccact gagcaacagc tcgaaacaca actgcagcag
     8461 accgaggaac agctcaacaa gctgcagagt gcgcaacccg gcggcaatga agccattctt
     8521 tcacctgaag cggccaacgc catcgagcgc ttccaggatg aaaaactgcg cattcgcaag
     8581 gaactgcgca agacgaaagc cgatctcgag ctcgacatca aggcgttggg tacgcgcctg
     8641 aaactcatca acgtgctcac gatgccgctg accatcatcg gcattggcct cctcgtgtac
     8701 ttctggcgca agcgccggcg ccatgccatt gccatgcttc gcaaggggag cgcggcatga
     8761 accagaaaaa gtttctcgca ttgaccgtca ccaccgtggt ggtgctggcc gcgggcgtgt
     8821 ggatctcgat gcaccgctcg agcaagcagt cggacctcgg tggcaccgca atgttcgccg
     8881 atctcaagcc ggcgctcggc gaagtttcgg aagtgcgctt cgcgaagggc gacggcagcc
     8941 gcaccacgct gcgcaaatct gccgatggct ggacagtggt cgaacgccaa tacccggccg
     9001 atgcttcgcg cgtgcgcgaa ctcgtgctga acctcgccag cctcaaggtg gtcgagcgca
     9061 agacgcagga cccggctaac tacgccagac tcggcgtcga ggcgcccgac agccccacgg
     9121 ccggcagcac gctggttgag gtaattgcgg acaaaaagac ctggtcactg atcgtcggca
     9181 agggcgccga cggcaaagcc atctatgtgc gcaaaccgga cgagacggtg agcctgctgg
     9241 cagctccttt catcacggtc gatccggatc agaagcgctg gatcgatcgg ctgctggtcg
     9301 acgttcccgg tgcgaacgtg cacgacgtct ccgtcaaact cgccaacagt ccggcctatg
     9361 tgctgacgcg tgccgcgcgc ggcgcccagg atctttccgt gagcccggtt ccgaagggcc
     9421 gcaaggccgc caccagcatg tcgctgggtt cgcagaccga agcgctggct gcattccact
     9481 tcgacgatgt acgcaccgca ccctcggcgg ccccaggcgc cagcgacacg gcgacctatc
     9541 gcatgtttga cggccaagtc atcgagttca caggacaccg cgaaggcgac aaggcattcg
     9601 tgacggtcgc cacgcgtcgc gatccggcgc tggcggcaaa attccccgag gcgccgcccc
     9661 cgcccgcgcc tccatccacg ccggcggctc ctgcaacggc gcccgcggcg acacccggtg
     9721 ccgatcagat cgcggaaaaa ctcggtgccc gagcgaaggg catggaattc gagattcccg
     9781 cgtacaaata cgaggcgatt ttccgcaagc cggaagaatt gctggagaag ccggccgagt
     9841 ctgtgaagga ctaggaaggg ggtgcggttt gggggggtgc gtgccccagc gagcagtccg
     9901 cgcacagaag ttcgttcgcc cttcgtaacg aggggcggtt tgggggtgtg cgggccccag
     9961 cgagcagtcc gcgcacagga tttcgttcgc ttttcgccgg gcccttcgtt cggggtggca
    10021 acgccagttt gcgggccgcc gccacacgcg atgacacttt cgtgcagttg gagttgtaag
    10081 gcggcgagag tccgcatgaa ttagagaggc ggcttcgtgc ctaacgaggc caggtatccc
    10141 ggcccgctct ctcagctcct gtgcgcggac tgctcgctgg ggctcatacg tcatctaacc
    10201 gcgccccgct ttcctgctgc ggtggtaaga caaagagccg cacggaattg aggtcttccc
    10261 accgcgtggg gttacacgcc tct
//
LOCUS       DASQJS010000431         6073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1536882, whole genome shotgun sequence.
ACCESSION   DASQJS010000431 DASQJS010000000
VERSION     DASQJS010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536882"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..518
                     /locus_tag="VEW08_16950"
     CDS             159..518
                     /locus_tag="VEW08_16950"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002019.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HYJ42481.1"
                     /translation="MSSSTDDTLINKACVPCHGGISALNRTEAERLLGQLLARNGDGW
                     KIIEDGKAIKREFKFPDFYRTMSFVNAVAHIANREDHHPDIECGWGYCRIRYQTHAIG
                     GLHENDFICAAKIDGLV"
     gene            600..2036
                     /locus_tag="VEW08_16955"
     CDS             600..2036
                     /locus_tag="VEW08_16955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HYJ42482.1"
                     /translation="MFDDTRKRWWALYVLCAGVLMIVLDTTIVNVALPSIREDLKFSE
                     TSLVWVVNAYMLTFGGFLLLGGRLGDLFGHRRLFLLGLILFTAASIGCGVATSQGMLV
                     TARAVQGLGGAVVTAVALSLIMDLFTENADRAKAMGIYGFVCSAGGSIGVLLGGFLTT
                     TLNWHWIFLVNVPIGALVYAFCVRLLPHAPGTASGARLDVAGAVTVTTSLMLAVYGIV
                     NGHEAGWTSPQTLATLGSSVVLLIAFLLIEARVRNPLMPLTLFRLRNVATANIVGVLW
                     AAAMFAWFFISALYLQRVLGYNAMQVGLSFLPANLIMAAFSLGLSAKIVMRFGIRTPL
                     AAGLGLAAAGLALFARAPLDGGFMVDVLPGMILLGIGAGIAFNPLLLGAMSDVAPSES
                     GLASGMVNTAFMMGGALGLAVLASFAAMRTDALLSAGSDGVTALNGGYQLAFLLGAAS
                     AAIAAIAGGIFMRTRAAPVPNGLVSGAE"
     gene            complement(2022..2465)
                     /locus_tag="VEW08_16960"
     CDS             complement(2022..2465)
                     /locus_tag="VEW08_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42483.1"
                     /translation="MAIEIVPKILLPTMDTERFERCSFSLQDAESLLTIHMSELPPFI
                     IHFHRLRWHRVTPNAQCTPVLTHGCQMAVAEVVNSPALIHYIARENVPAAKTRALHHY
                     RIYLGEGGCHEAFAQSAYWSGDGKVSRAYASFANGVRMRLRSLGS"
     gene            complement(2531..3397)
                     /locus_tag="VEW08_16965"
     CDS             complement(2531..3397)
                     /locus_tag="VEW08_16965"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ42484.1"
                     /translation="MKQHRLAGMAAMLAGVAAFSLMDAGLKLLSNDYPPMQVAALRGL
                     SALPLVFAWAMYAGGPKQLLNVRWPLHLIRGVLSVIMMATFVFGIQQLSLAKTYALFF
                     VAPLLIAILSVLTLGERVSRSQWVAIAIGFMGVLFVRPPEIVGWAAPLAILGTALCYA
                     LSSILVKIIGRTDTTQSMVFWMTCMLAVGATVLTLLAPQGWQAIHREHYLIIGGIAVT
                     GAVGQWGITKAFKHAPAASVAPLEYSGMAWVILIDFFVWSVMPEWQTLVGAAVIIGSG
                     LYLLRFESRRSN"
     gene            complement(3420..4646)
                     /locus_tag="VEW08_16970"
     CDS             complement(3420..4646)
                     /locus_tag="VEW08_16970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYJ42485.1"
                     /translation="MSIDYDVAVVGAGPIGLAAAILVARQSGIPPARVAVFDRRIPEP
                     LDRTRELPIDLRVFALSRASERILRAADAWSDIAATRAEAFERMQVWHADVPPHGGDA
                     LVFDAAEMGERNLGVIAENAVLQAALAGAACRAGIALVTGEISALDQERDAVVLTAGE
                     RRVRVRLVIGADGAQSRVRELAGLGAARADYGQTAIVAMVSTARPHEHTAWQRFLGDG
                     TLAFLPLKDANSSIVWSVPTARAERLLAIAPEAFERELESDFDGALGKVRLVSERLKF
                     PLWRLSADAYVTSRVALVGDAAHVVHPLAGQGANLGLLDAAALADVLAEGVKGREDPG
                     AERILRRYERWRRSENDLMSSAIDAFDRLLARGTGRVAEIAQRGMPWVGRSTLAKRMF
                     IERAMGLAGELPVAAR"
     gene            complement(4643..5866)
                     /gene="ubiH"
                     /locus_tag="VEW08_16975"
                     /gene_synonym="visB"
     CDS             complement(4643..5866)
                     /gene="ubiH"
                     /locus_tag="VEW08_16975"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF004356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HYJ42486.1"
                     /translation="MKEWDVVIVGGGMVGASFALALRATPLRVLLVEGVPPDSAAQPS
                     FDERTTALGNGSRQIFETLGVWPEMSGDASPIRSIHVSDAGRFGVARLDAHEQGVQAF
                     GYVVPNRVIGRALWKALREAPNITIAMPAQLKSATLREDGVLLDLGLEGITEKVRAAV
                     AVAADGAGSVLRASAGIEAAVENYDQVAIVVNAATERPHTGEAFERFTASGPLAVLPV
                     TGGGYAVVWAVSPARAAELISLDEAGFAAELLTAFGWRAGRWTRIGRRNSYPLTLTRA
                     EETVSGRVVLIGNAAQALHPVAGQGFNLGLRDAATLAEMLASGASNSDFALTTSELLE
                     KFAAWRAEDRKGVTRFTDGLVKLFGSDTPGVGLVRNFGLLLFDMSPAAKRALSRVSWG
                     FAGRMPRLARGLPLE"
     gene            complement(5882..>6073)
                     /locus_tag="VEW08_16980"
     CDS             complement(5882..>6073)
                     /locus_tag="VEW08_16980"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HYJ42487.1"
                     /translation="GMALTIEPGIYVAPGTKGAPKKFQGIGIRIEDDVVVTRDGCEVL
                     TEHCPKEAVEIERLMASVA"
BASE COUNT         1056 a         2045 c         1936 g         1036 t
ORIGIN      
        1 ggcgagcggc ccgtggcgcc ggtctacatc gatggcgaga aggggccgac gctcaaaggc
       61 gagcgtattg ccgaggaatt ccaggacctg gtcgagaaat acgtggcgcg gcgcttcacg
      121 cgcaagaccg acgtggcggc cgagcaagga gtcacagcat gagcagcagt accgacgaca
      181 ccctgatcaa caaggcctgt gtgccctgcc atggcggcat cagcgcgctc aaccggaccg
      241 aagcagaaag gttgttaggc cagctcctgg ccaggaatgg cgacggctgg aaaatcatcg
      301 aagacggcaa ggcgatcaag cgtgaattca agttcccgga tttctaccgc accatgagct
      361 tcgtgaacgc cgtggcgcac atcgccaacc gcgaagatca ccatcccgac atcgagtgcg
      421 gctggggcta ctgccgcatc cgctaccaga cgcacgccat cggcgggttg cacgagaacg
      481 atttcatctg cgccgccaag attgacgggc tggtctgagt tcatgcgtca tgtcaccgct
      541 cggacgacgc cgcttcggcg gcgttttccg gcgtgggcgt cagccctgag gaagtagcca
      601 tgttcgatga cacacgcaag cgctggtggg cgctgtatgt cctttgcgcc ggcgtattga
      661 tgatcgtgct cgacacgacc atcgtgaatg tcgcgctgcc ctcgatccgg gaagacctga
      721 agttttccga aacctctctg gtgtgggtgg tcaacgccta catgctcacc ttcggcggtt
      781 tcctcttgtt aggcgggcgc ctgggcgacc tcttcggtca tcgccgcctg ttcctgctgg
      841 gattgatcct gttcacggcg gcatcgatcg gctgcggcgt tgccacttcg caggggatgc
      901 tggtcacagc gcgtgccgtc cagggcctgg gaggcgcggt cgtgaccgcg gtggcactgt
      961 cgctcatcat ggacctgttc acggagaacg cggatcgtgc gaaggccatg ggcatctacg
     1021 gcttcgtctg ctcggccggc ggcagcatcg gcgtgctgct cggcggattt ctcaccacca
     1081 ctctcaactg gcactggatc ttcctcgtca acgtgcctat cggcgccctc gtctatgcgt
     1141 tctgcgtgcg cctgctgccg cacgcgccag gaacggcgag cggcgcaagg ctcgatgtcg
     1201 caggcgccgt gacggtgacc acctcgctca tgctggcggt ttacggcatc gtcaacggtc
     1261 acgaagccgg ctggacgtcg ccgcagacgc tcgccacgct cggaagctcc gtcgtgttgc
     1321 tgatcgcctt cctcttgatc gaggcacgcg ttcgcaatcc tctgatgcca ctcaccctct
     1381 ttcgcctgcg caatgttgcc actgcaaaca ttgtgggtgt gctctgggca gcggcgatgt
     1441 tcgcgtggtt cttcatttcc gcgctctacc tgcagcgcgt gctgggctac aacgctatgc
     1501 aggtgggtct ctcgttcctg ccggccaatc tcatcatggc ggcgttttca ctcgggctct
     1561 ccgcgaagat cgtcatgcgt ttcggcattc gcacgccact cgccgcaggc ttaggcttag
     1621 ccgcggctgg cctcgctctc tttgcgcgcg cgccgctgga cggtggattc atggtggacg
     1681 tgctgccggg catgatcctg ctcggaatcg gcgcgggcat cgcgttcaat ccgctgttgc
     1741 tgggtgcgat gagcgacgtg gcgccgagcg aatccggcct cgcctcgggg atggtgaaca
     1801 cggctttcat gatgggtggc gcactcggtc tcgcggtgct cgcgagtttt gccgccatgc
     1861 gcaccgatgc gctgctgtcc gcggggtccg acggagtcac ggcattgaac ggcggctatc
     1921 agctcgcgtt cctgctcggc gccgcaagcg cagccatcgc ggcaatcgcc gggggcatat
     1981 tcatgcgtac ccgcgccgca cccgtgccga acggcctggt gtcaggagcc gagtgatcgc
     2041 aaccgcatcc tgacgccatt ggcgaacgac gcatacgcgc gcgatacctt gccgtcaccg
     2101 ctccagtacg ccgactgcgc aaaggcctcg tggcagccgc cctcgccgag atagatgcgg
     2161 tagtggtgta gcgcccgggt cttcgccgcc ggaacattct cgcgcgcaat gtagtggatc
     2221 agcgccgggg agttgacgac ctcggccacg gccatctggc aaccgtgagt gaggaccggc
     2281 gtgcattgcg cattcggcgt gacgcggtgc cagcgcaacc ggtggaaatg gatgatgaac
     2341 ggcggcagct cgctcatgtg aatcgtgagc agcgactcgg cgtcctgcag cgagaagctg
     2401 caacgctcga agcgctccgt atccatcgtc ggcagcagga ttttggggac gatctcgatc
     2461 gccatgcggc gaagtattgc cgaacggcca gcggcgcgga cggccgggcc tgaggaactg
     2521 tgaccggcgc ctaatttgaa cggcgcgatt cgaaacgcag caggtacagg ccgctgccga
     2581 tgatcacggc ggcgcccacc agcgtctgcc attcgggcat caccgaccaa acaaagaaat
     2641 cgatcaggat cacccatgcc atgcccgaat attcgagcgg cgccacgctg gccgcgggcg
     2701 cgtgcttgaa cgccttggtg atgccccatt gcccgactgc gccggtgacg gcgattccgc
     2761 cgatgatcag gtagtgctcc ctgtgaatgg cttgccagcc ctgcggagcg agcaacgtca
     2821 gaaccgtcgc gccgacggcc agcatgcagg tcatccagaa caccatcgac tgcgtggtgt
     2881 cggtccgccc gatgatcttc acgaggatcg acgacaacgc ataacacagc gcggtgccca
     2941 atatggcgag cggtgccgcc caacccacga tttcgggcgg acgaacgaag agcacgccca
     3001 tgaacccgat agcgatagct acccactgcg agcggcttac gcgctcaccc agcgtgagaa
     3061 ccgaaaggat tgctatcagc aggggtgcaa cgaagaacaa agcgtaagtc ttggccagcg
     3121 acagttgctg aattccgaag acgaacgtcg ccatcatgat caccgaaagt acgccgcgaa
     3181 tcaggtgcag cggccagcgc acgttcagca gctgcttcgg cccgcccgca tacatcgccc
     3241 acgcgaaaac caggggcagc gcggacaatc cgcgaagtgc ggcaacctgc atgggcggat
     3301 agtcattcga cagcagcttc aggcccgcat ccatcaatga gaacgccgcg acgcccgcga
     3361 gcattgccgc cattcccgcc aggcggtgct gtttcatgtc ttgcatggcg ttcttgcggt
     3421 cagcgcgcgg ccactggcag ctcgccggcg aggcccatcg cgcgttcgat gaacattcgc
     3481 ttcgccagcg tggaccgacc gacccatggc atgccgcgct gcgcgatctc ggcaacgcgg
     3541 cccgttccgc gcgccagcag gcgatcgaac gcgtcgatgg cgctgctcat cagatcgttc
     3601 tcggaacgcc gccagcgttc gtagcgccgc agaatgcgct ctgccccggg atcctcgcgg
     3661 cccttcacgc cttcggccag cacatcggcc agcgccgcgg cgtctaacag cccgagattc
     3721 gcgccctgtc cggccagcgg atggaccacg tgtgcggcgt cgcctaccag ggccacgcgc
     3781 gaggtgacgt atgcatcggc cgaaagccgc cacaatggaa acttcaggcg ttcgctcacc
     3841 aggcgcactt tgccgagcgc accgtcgaaa tcggattcca gctcgcgttc gaaagcttcc
     3901 ggcgcgatgg ccagcagccg ctcggcgcgc gccgtgggga ccgaccatac aatggagctg
     3961 ttcgcgtcct tcaacggcag gaaggccagg gttccgtcgc cgaggaagcg ctgccatgcg
     4021 gtgtgctcgt gcggccgggc cgtgctcacc atggcaacga tggcggtctg cccatagtcg
     4081 gcacgcgcgg cgcccagccc agccagctca cgcacgcgcg actgggcgcc atcggcaccg
     4141 atcaccagcc gtacacgcac ccggcgttcg ccggcagtca gcaccacggc gtcacgctcc
     4201 tgatcgagcg cgctgatctc accggtgacg agcgcgattc cggctcggca cgccgcgcca
     4261 gccagtgccg cctgcaatac cgcgttctcg gcaatgacgc cgaggttgcg ctcgcccatt
     4321 tccgcggcat cgaacaccag cgcgtcaccg ccatgcggcg gaacgtcggc gtgccacacc
     4381 tgcatgcgct cgaaagcctc cgcgcgcgtg gcggcgatgt cgctccaggc gtccgcggcc
     4441 cgcaggattc tttcgctggc ccgcgacagc gcgaacacgc gcaggtcgat tgggagttcc
     4501 ctggtccggt cgagtggctc gggaatgcgc cggtcgaaca ccgcgacgcg cgccggcgga
     4561 atacctgact ggcgcgcgac gagaatggcc gccgccagcc cgatggggcc cgcgcccacc
     4621 acggccacgt cgtagtcgat gctcattcga gtggcaggcc gcgcgccagc cgcggcatgc
     4681 ggccggcgaa gccccagctc actcgcgaca acgcacgttt ggcagcggga ctcatgtcga
     4741 acaacagcag gccgaagttg cgcaccaggc cgacgcccgg cgtatcgctg ccgaaaagtt
     4801 tcacgagacc gtcggtgaag cgcgtaaccc cttttcgatc ttccgcgcgc catgcggcga
     4861 acttctcgag cagctcggac gtcgtgagtg cgaagtcgga attcgacgcg ccactcgcca
     4921 gcatttccgc cagggtggcg gcgtcgcgca gccccaggtt gaaaccctgg cctgcgaccg
     4981 ggtgcagcgc ctgtgcggcg ttgccgatga gcacgacgcg gccggacacg gtttcttcgg
     5041 cgcgcgtcag cgtgagtgga taggagttgc ggcgcccgat gcgcgtccag cggcccgcgc
     5101 gccagccaaa cgcggtgagc agctcggcgg cgaacccggc ttcgtcgagt gagatcagtt
     5161 ccgcggcgcg cgcggggctc accgcccaca ccacggcata cccgccgccg gtcaccggca
     5221 aaaccgcgag cggaccagac gcggtaaatc gctcgaaggc ctcacccgtg tggggtctct
     5281 ccgtggcggc gttcaccacg atggcgacct ggtcgtagtt ctcgactgcg gcctcgatgc
     5341 ccgcgctggc gcgcaacacc gaccccgcgc catccgcggc caccgccacc gcggcgcgaa
     5401 ccttttcggt gatcccttcg agacccaagt ccagcaggac gccatcttcg cgcaatgtcg
     5461 cgctcttcaa ctgcgcgggc atggcaatgg tgatgttggg cgcttcgcgc aatgccttcc
     5521 acaacgcgcg gccgatgacg cgattcggaa cgacgtagcc gaaggcttga acgccctgct
     5581 cgtgcgcatc gagccgcgcg acgccgaagc gtccggcgtc ggatacatga atggagcgaa
     5641 tcggcgaagc gtcgcccgac atctcgggcc aaacgcccag agtctcgaaa atctgccgcg
     5701 acccgttgcc gagcgccgtg gtgcgttcat cgaagctcgg ttgcgccgcc gaatccggcg
     5761 gcacgccttc gacgagcagc acgcgcagag gggtggcacg caatgccagg gcgaagctgg
     5821 cacccaccat gccgccaccg acgatcacga cgtcccactc tttcattgaa tggctttcat
     5881 ttcacgcgac cgacgccatg agccgctcga tctcgacggc ttccttcggg caatgctccg
     5941 tgaggacttc acaaccatcg cgggtcacta ccacgtcgtc ctcgatgcgg atgccgatgc
     6001 cctggaactt cttcggcgcg cccttcgtgc ccggggccac gtagatgccc ggctctatgg
     6061 tgagcgccat gcc
//
LOCUS       DASQJS010000432         4676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3112211, whole genome shotgun sequence.
ACCESSION   DASQJS010000432 DASQJS010000000
VERSION     DASQJS010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3112211"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..3399
                     /locus_tag="VEW08_16985"
     CDS             28..3399
                     /locus_tag="VEW08_16985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYJ42488.1"
                     /translation="MNARPESTLAPAAPLPAEFRSALELAQAAVWDWHVSADHFQVDE
                     PWLRALGIDVHASAMPCAEWKRRIHPDDLGAFIAATDSCHHGGDRFECEYRLLAAGHR
                     WLWVLHRGRVVLRERDGSAQRIAGLLLDIDRRKSEEVARSDSESRLATALWGARAAFW
                     QWHVPSNARTSSPLWLAMTGYSRAQWDVMPNPWFSNLHPDDRERVDRQLQEHAQGERD
                     SVEFEYRFQTAGGEYRWMQDRGRAVEWDLQGRPVLVIGVTLDIDAAKHAEAHLRSSES
                     MLETAAWGAGIGLWETNFITDKTRWFNDWCDRHDIHPCDGDDHVQRWDSYLHPDEGPE
                     ATRRFSEHVAGKAEYYDAEYRIKTRSGAWRWVFERGRVVERDARGKAVRMLGVCMDLD
                     ETKVAERRANARNERVEAALQLTTAGVWDWDVEHGLTNDTDGYYRVFGVDPAFARANI
                     LTWRQLLGTDPDCEIERFRERLLHVDSAAEVLETEYRFRHTDGSWHWALDRAYVVDRA
                     PDGSTRRVLGLVVDITARKIRETGLSAADQRFRAIARELRCVIYEIEAETGMSTGEGV
                     ERVLGYSNADLPRPGDWAALVHPDDQPLLRQWFDNRAESMVALQYRIRHRDGHYITLL
                     DSPCVVRDAAGKVVRIVGVAIDISEQASAQEALRASQELLQIVAAGTGDWLILVDSQR
                     CVQFINRDIASHSRESIIGRPLDEIAEPADRQDILAALTQVLETGEPVDLQMASNGKD
                     GRIFDSRVRAVRSPNGITGAVINITEITDRQAAMHLRETQARMFELLHEGVVVIDVNN
                     MIRMANPAFERMFGFTAGAAVDTSIGDLIAQPPGPLRERLDQQLLGNIAEPPGLTPVE
                     FKCRRRDGSTFEAACVATLTHVDGATHRLAVITDVTERRGLEREILEIAGREQLRIGS
                     DLHDGLGQDLTGVALMLRSVVAQLRKENSTARTDVEDIITLVNGAIESTRAMARGLAP
                     VGADRGGLIAGLQSMAVRGMERYGVRAHFNTSLKEPLTLDDGAATHLYRIAQEAFTNA
                     IRHGRVTQVTIELATAEGTLTLSVQDNGRGFDERNASNNGMGMKLMRYRAQMLGGDVS
                     IANNKGGGVIVRCTCPHRAPLGGEKLPRGGFPLKD"
     gene            3515..4186
                     /locus_tag="VEW08_16990"
     CDS             3515..4186
                     /locus_tag="VEW08_16990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ42489.1"
                     /translation="MTAAMATKRQILIVDDHPIVRQGLRRMFEAEPDLTVCGEADSEP
                     AAREAIRARKPDIVVVDLSLERGDGIELVRQMHAEMPELRMLVLSMHDETIYAERLLA
                     AGAMGYIMKQAASDQLLIAVRRVLDGRTYVSETVQRSLDQRAGENRSETNDPVARLSN
                     REVQILNMIGRGMSSREIAQEFTLSVKTIESHRQTIKHKLGLATNSQLLQYAMKWFSG
                     GTQRS"
     gene            4340..>4676
                     /locus_tag="VEW08_16995"
     CDS             4340..>4676
                     /locus_tag="VEW08_16995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYJ42490.1"
                     /translation="MADGATARMTIADKGRADTVVDFDVALIERARKGDSKAFERLYR
                     DHVGRVHGLCLRMTRNPDQAADCAQDAFIKAWKALPKFEARSSFSTLLHRIAVNTVLE
                     KRRGPSNAEI"
BASE COUNT          884 a         1515 c         1506 g          771 t
ORIGIN      
        1 gcggctcgca gataacaata atcggtcatg aacgctcgcc ccgaatccac gctcgctcca
       61 gccgcgcctc tgcccgccga atttcgttcg gcgctcgagt tggcgcaggc ggcggtttgg
      121 gactggcatg tctccgcgga tcactttcag gtcgacgagc catggctgcg cgcgttgggc
      181 atcgacgtgc atgcgagcgc catgccctgc gcagaatgga agcggcgcat ccatccagac
      241 gatctcggcg cgttcatcgc ggccaccgat tcctgtcacc acggtggcga ccggttcgaa
      301 tgcgaataca ggttgttagc cgcggggcat cgctggctgt gggtcctgca tcgcgggcgc
      361 gtcgtgctgc gcgagcgcga tggatccgcg cagcgcatcg ccggattgct gctcgacatc
      421 gaccggcgca agagcgaaga ggtggcgcgc agcgacagcg agtcgcgcct cgcgaccgcg
      481 ttgtggggcg cgcgcgccgc attctggcag tggcacgtac ccagcaatgc ccgcacctcg
      541 agtccgttgt ggctggcgat gacgggctac tcgcgggcgc agtgggacgt gatgccgaac
      601 ccgtggttca gcaacctgca tcccgacgat cgcgagcgtg tcgaccggca gctgcaggaa
      661 cacgcgcagg gcgagcgcga ctcggtcgag ttcgaatacc gctttcagac cgctggcggc
      721 gagtatcgct ggatgcagga tcgcggccgc gcggtcgaat gggacctgca gggacgcccg
      781 gtgctggtca tcggcgtcac gctcgacatc gacgccgcca agcacgccga agcgcacttg
      841 cgttccagcg aatccatgct ggaaaccgcc gcgtggggcg ccggcatcgg cctgtgggaa
      901 accaacttca tcaccgacaa gacgcgctgg ttcaacgact ggtgcgaccg tcacgacatc
      961 catccgtgcg acggtgacga tcacgttcag cgctgggact cgtacctgca tcccgacgaa
     1021 ggtccggagg cgacgcgccg cttttccgaa cacgtcgccg gcaaggccga gtactacgac
     1081 gcggaatatc gcatcaagac ccgctcgggc gcctggcgct gggtgttcga gcgcggccgc
     1141 gtggtcgagc gcgacgcgcg cggcaaggcc gtgcgcatgc tcggcgtgtg catggacctc
     1201 gacgaaacca aggtcgccga gcgccgcgcg aatgcgcgca acgagcgcgt cgaggcggcg
     1261 ctccaactaa ctaccgccgg tgtctgggac tgggacgtgg aacacgggct cacgaacgac
     1321 accgatggct actaccgcgt gttcggcgtg gatccggcgt tcgcgcgcgc caacatcctg
     1381 acctggcggc agctgctcgg caccgacccg gactgcgaga tcgagcgatt ccgcgagcgc
     1441 ctgctgcacg tcgactccgc cgccgaagtt ctcgaaaccg aatatcgctt ccgccacacg
     1501 gatggaagct ggcactgggc gctcgatcgc gcgtatgtcg tggaccgcgc gccagacgga
     1561 tcgacgcgcc gggtgctcgg tctcgtggtc gacatcaccg cgcgcaagat ccgcgagacc
     1621 ggactgtcgg cggccgacca gcgcttccgg gcgatcgccc gcgaactgcg ttgcgtgatc
     1681 tatgagatcg aagcggaaac cggcatgtcc accggcgaag gcgtcgagcg cgtgctcggc
     1741 tacagcaatg ccgaccttcc gcgccccggc gactgggcgg cgctggtgca tccggacgac
     1801 cagccgctgc tcaggcaatg gttcgataac cgcgccgaat ccatggtcgc gcttcaatac
     1861 cgcatccgac atcgcgacgg ccactacatc acgttgctgg attctccctg cgtggtgcga
     1921 gacgccgccg gcaaggtggt gcgcatcgtc ggcgtggcca tcgatatcag cgagcaggcg
     1981 agcgcccagg aggcgttgcg cgcctcgcag gaattgctgc aaatcgtggc ggccggcacc
     2041 ggtgactggc tgatcctggt cgactcgcag cgctgcgtgc aattcatcaa tcgcgacatc
     2101 gcctcacatt cgcgcgaaag catcatcggc cggccgctcg acgagatcgc cgaacccgcc
     2161 gaccgccagg acatcctcgc ggcgctcacc caggtcctgg agaccggcga gccggtcgac
     2221 ctgcaaatgg cgagcaacgg caaggacgga cgcatcttcg attcgcgggt gcgtgcggtt
     2281 cgctcaccga acggcatcac cggtgcagtg atcaacatca ccgagatcac cgaccgccag
     2341 gcggccatgc acctgcgcga gacccaggcg cgcatgttcg agctgctgca cgaaggcgtg
     2401 gtggtgatcg acgtcaacaa catgattcgc atggcgaatc ccgccttcga gcgcatgttc
     2461 ggtttcaccg caggcgccgc cgtcgacacg tccatcggcg atctcattgc acagcctccg
     2521 ggcccgctcc gcgagcggct cgatcaacag ttgctgggaa acatcgccga gccgcccggc
     2581 ctcacgccgg tcgagttcaa atgccgccgg cgagatgggt ccaccttcga ggcggcctgt
     2641 gttgccacgc tcacgcatgt cgacggcgcc acgcaccggc tcgcggtcat taccgacgtc
     2701 accgaacgcc gcgggctcga gcgtgagatc ctggagatcg ccggccgcga acaattgcgc
     2761 atcggctcgg acctccatga cgggttggga caggatttga ctggtgttgc gttgatgttg
     2821 cgcagtgtgg tcgcgcagct acgcaaggag aactcgacgg cgcgtaccga cgtcgaggac
     2881 atcatcaccc tggtgaacgg cgcaatcgag agcacgcgcg caatggcgcg cggcctcgcg
     2941 cctgtgggcg cagatcgagg tggtctcatc gccggcctgc agtccatggc tgtgcgggga
     3001 atggagcgat atggcgtccg ggcccacttc aatacctcgc tcaaagagcc tctgacgctc
     3061 gatgatggtg ctgcaacgca cctttaccgc atcgcccagg aagctttcac caacgcaatc
     3121 cgccatggac gcgtcaccca ggtcacgatt gagctcgcca cggcagaagg aacgttgacc
     3181 ctcagcgttc aggacaacgg taggggtttc gatgaacgaa acgcgagcaa taatgggatg
     3241 gggatgaagc tgatgcgcta tcgcgcacaa atgctgggcg gggacgtcag catcgcgaac
     3301 aacaagggcg gaggcgtcat cgtccgatgc acctgtccgc atcgcgcacc cctcggcggg
     3361 gagaagctgc cgcgcggcgg attcccgtta aaggattgat tgacccaatt aaatcgggtc
     3421 taactatagt ggcgtatacc tatgtcgaag cattgacgct ccgcagtatc cgcgtcacga
     3481 ttcacgccta ataaatgggc gtaggggatc gaacatgacc gcagcgatgg ccaccaagcg
     3541 ccagattctc atcgtcgacg accatccgat cgttcggcag gggcttcggc gcatgttcga
     3601 ggcggagccg gacctcacgg tctgcggcga agccgacagt gaaccggccg cccgcgaagc
     3661 cattcgtgcc cgcaaaccgg acatcgtggt ggtggacctg tcgctcgagc gcggtgatgg
     3721 catagaactt gttaggcaga tgcacgccga gatgcccgag ctgcgcatgc tggtgttgtc
     3781 catgcacgac gaaaccatct atgccgaacg cctgctcgcg gctggcgcca tgggctacat
     3841 catgaagcag gcggcttccg accagctgct catcgcggta cgacgcgtgc tcgatggacg
     3901 tacctatgtc agtgagaccg tccagcgttc cctcgatcag cgcgccggtg agaaccgctc
     3961 ggaaaccaac gatcccgtcg cgcgcctgtc gaaccgcgaa gtgcagatcc tcaacatgat
     4021 cggccgcggc atgagctcgc gcgaaatcgc gcaggaattc accctctcgg tgaagaccat
     4081 cgaatcccat cgccagacca tcaaacataa actgggcctg gccaccaaca gccagttgct
     4141 ccagtacgcc atgaagtggt tctcgggcgg gacacagcgc agctaagacg gtgccgggcc
     4201 gggaaaccgg gaaaactccc agtcatttcg ccgaattgtc gcgctccggc cggagttttc
     4261 ccggtttccc ggcccggcac cggcatttca aacccatgcc gcctacacgt cctctaaggt
     4321 acatacgagg gagagacgtt tggcagatgg tgcgacagcc cggatgacga tcgctgacaa
     4381 aggcagggcc gacacggtcg tcgatttcga tgtggcgctc atcgagcgcg cacggaaggg
     4441 cgattcaaag gccttcgaac gtctctaccg cgaccacgtg ggtcgcgtgc acgggctttg
     4501 cctgcgcatg acgcgcaacc cggatcaggc ggccgactgc gcccaggatg ccttcatcaa
     4561 ggcctggaag gcgctgccga aattcgaggc gcgcagcagc ttctccacct tgttgcaccg
     4621 tattgcggtg aataccgtgc tcgagaagcg gcggggcccc tcgaacgcgg aaatcg
//
LOCUS       DASQJS010000433         9366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_286631, whole genome shotgun sequence.
ACCESSION   DASQJS010000433 DASQJS010000000
VERSION     DASQJS010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9366
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286631"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VEW08_17000"
     CDS             <1..545
                     /locus_tag="VEW08_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42491.1"
                     /translation="FFVGALLGMMLPALLYVTFVAPGTDIKGLAISAVLAQTIGATAG
                     PWLGLCVAGLGVWILFKTQLDCVEGMTRSITDILWTGSARVRRWRGGDVRAVYYTVLA
                     VVVVWGIIALKLAAPVMLLQIGANIASAIFVIASLHVMYLNTRILPIALRPPLWRRLA
                     LVGMALFYGFFVVLVTRSLL"
     gene            complement(549..1160)
                     /locus_tag="VEW08_17005"
     CDS             complement(549..1160)
                     /locus_tag="VEW08_17005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ42492.1"
                     /translation="MPLVDPVIERVLAEFERRAEEEQRRFSAPGTNRDDLLLPVGREA
                     GILLYLLATGAHSKRILELGSSYGYSTVWLGAAARTTGGKVQSLELKENKIEHARQAL
                     TRAGLSSRVEFYAGDCLDSLRSLPGPFDFVLLDVWKDLYLPCFELVHPKLAMGGIIAA
                     DNMLLPESARPHADAYRQRVRAAGDMDSVLIEVGNGIEISRKR"
     gene            1531..2772
                     /locus_tag="VEW08_17010"
     CDS             1531..2772
                     /locus_tag="VEW08_17010"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYJ42493.1"
                     /translation="MRNALVRVVSVLGLGCIAPGTAIAADNDGLALPPGFSAVVVHEG
                     QGTARHLDVAANGDIYVAGRNGLSAMRDTNRDGKADIVATFGDVKGTELRLFRNWLYV
                     SDDVGVYRYALAKAQLAPAGSRETVVSEFPKERQHSDKTFALDAKGTLYINVGAPSNS
                     CQEKDRQEGSLGLNPCPILEKYGGVWVFDGTKLNQTPANGRRFATGMRNAVAIEWNAT
                     QNSLFAVIHGRDSLDTLFPTLYTAEDNATRQAEEFHQIVDGGDYGWPYTFFDTRLARR
                     VLAPEYGGDKTKEPEAGKYPEPLVAFPAHWAPNDLLFYSGKSFPAKYREGAFIAFHGS
                     WNRAPEPQAGYKVVFQPMKDGKANGPYEVFADGFAGDMKDNSPRNAQYRPVGLAVGPD
                     GSLFIADSQKGRVWRISYRGK"
     gene            2769..3185
                     /locus_tag="VEW08_17015"
     CDS             2769..3185
                     /locus_tag="VEW08_17015"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYJ42494.1"
                     /translation="MSARSLRLAAVFAGLWLAAASSVANEPAPGQLLYNKHCLACHQA
                     DGGGVPNMQPAIAGGTWVKGDARALALFVMSGGFDSASRKDSDVGNVMPPFHQLPDAD
                     LAAILTFIRQKFGDGGSPVSAADVADARTSLPASPK"
     gene            complement(3219..3965)
                     /locus_tag="VEW08_17020"
     CDS             complement(3219..3965)
                     /locus_tag="VEW08_17020"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYJ42495.1"
                     /translation="MKTLLASALLCLAAPVFAADATPIESAIASPERTDKDRERDARE
                     KPAEVLAFAGVKPGMTVVDMFAGGGYYTELLAGVVGPSGKVLSVNNVPYEQYSKDAVK
                     ARFTEGRLKNVERRLVEVSSIHFPPKSVDLIVIVMSYHDAFWVDEKEGWPDIDTDGFI
                     ESMKHMLKPGGKLLIVDHNAPAGTGAEMASKAHRLNEEFAKKSLTSHGFVLEKTFDGL
                     RNKDDQLDKLVFDAAVRGKTDRYVHLYKVK"
     gene            4157..4774
                     /locus_tag="VEW08_17025"
     CDS             4157..4774
                     /locus_tag="VEW08_17025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ42496.1"
                     /translation="MPNRSSGSASRLDKPVTPEDQARNDYNAGVRLVEKADELAADAL
                     HQADERKKAKALSKAEQNYNTAMKKFSNATQLQPSNYQAWNYIGYTNRKVGKFDAALA
                     AYDRALKLKPDYAEAIEYRGHAYLGLNRLAEAKAAYLALFAGNRQLAATLLAAMQEWV
                     GKHRGSAEGVDGATLDSFASWVNERSTIASQTAALTREGAASAWH"
     gene            4774..5775
                     /locus_tag="VEW08_17030"
     CDS             4774..5775
                     /locus_tag="VEW08_17030"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HYJ42497.1"
                     /translation="MNGVAGRVAAIAVTTVLAVMVTTTAFAGEFRWQLPAGFPEPAVP
                     ADNPMSAAKVDLGAKLFADPRLSITGQYSCRSCHSPERAFTDGLAHSRGATGEELLLN
                     APTLLNVAYNSSLGWRDANLRTLERQMHGPLFNKDPRELGLAGREALVENALLADQEM
                     VRAFGAAFPGMDEPVSMDNVIRAIAAYERTLFAGYSAFDRYVFGGDHDALSERQKAGM
                     QLFFSGRTGCAACHGGVNFAGAWVDRDHPQAEPVFADTGTGEAVRVPTLRNLPATAPY
                     MHDGRFANLDQVLDHYESLAVDPAADPRLRRNPLTTSERESLREFLLSLADGLADSR"
     gene            5783..6364
                     /locus_tag="VEW08_17035"
     CDS             5783..6364
                     /locus_tag="VEW08_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HYJ42498.1"
                     /translation="MRASMHPDLTLGRHILLPHPVSVPGALQDVVHELSVLVARPEAD
                     QLNLTYRLSGDLDALQLPEPRPPVRTDGLWRRSCFEAFIAHAGGPDYWEYNFSPSGAW
                     AAYHFSAYREGMSPLLKGAAPAVTPRVRTDSVELSVRLDLSWLVRGGGVGLRLGLASV
                     VEDKARVLSYWALKHPAEKPDFHHSDSFVVELD"
     gene            6398..7606
                     /locus_tag="VEW08_17040"
     CDS             6398..7606
                     /locus_tag="VEW08_17040"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HYJ42499.1"
                     /translation="MIQFGIDRLLGDLKLQRELEGRRVALLAHPASMTADFEHSLDAL
                     RAIRGLDITAAFGPQHGLRGDKQDNMIESPDFRDPVHGIPVFSLYGEVRRPTEDMLDH
                     FDVLLVDLQDVGCRIYTFITTLLYVLEAAAKHGKTVWVLDRPNPAGRPVEGLSLRQGW
                     ESFVGAGPIPMRHGLTLGELGMWFVSHFHLAVDYRVIPMRGWKPDEEPGFGWPLGGRN
                     WVNPSPNAANLSMVRAYAGTVMLEGTTLSEGRGTTRPLEMLGAPGLDPRSLLTMMSRI
                     MPAWMTGCRLRPCWFEPTFQKHSGKLCSGFQIHVEDGAYEHGEFRPWRLIAGAFKCIR
                     RLRGEYPLWRDFPYEYEHSRLAIDLINGSELLREWVDDPAATPADLDRLAAVDEAAWE
                     EQRADLLLYR"
     gene            complement(7640..8020)
                     /locus_tag="VEW08_17045"
     CDS             complement(7640..8020)
                     /locus_tag="VEW08_17045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HYJ42500.1"
                     /translation="MTRLNSASVLRRTSEHLTAGAAAIAVTFLMLLTVEVRAAETTDS
                     PPRISVTYQDTAFATPEGAANVYRKLKMAARKVCGLAPGNALNLDQRTKAQECVEGAL
                     ADAVQRINRPMLTSVHESSARDLG"
     gene            complement(8268..9266)
                     /locus_tag="VEW08_17050"
     CDS             complement(8268..9266)
                     /locus_tag="VEW08_17050"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HYJ42501.1"
                     /translation="MAAKKKAAKSSAKRKPKLAPNAVKRGLSATEVALALDHEDIAAV
                     VELVRKAGGAPIGAYREPLGGRPLLVASLPFNAVQPTPFQRDLSPTHAKKLAAAIEAT
                     AAFLDPLIVVRGEDGRFWTPNGRHRLAAAKVLGLKQITVLISPDETLSYRILALNTEK
                     AHNLKDKSLEVVRMARNLAKREPTRTEASLAAEFEAAELLTLGLVYEENLRFGGGAYS
                     PVLKKVDRLTTRPLPAALRERSAYAARLQEIDAEVKRIVAELQARGMKSPYLRSYVVA
                     RINPVRFHKVKPGDKKPVMPIAQALLRMSAAAKKFDLGKVNMSDLAFVAAGAESAE"
BASE COUNT         1616 a         2937 c         3046 g         1767 t
ORIGIN      
        1 ttttcttcgt cggcgccttg ttaggcatga tgctgccggc gctgctgtac gtgaccttcg
       61 tcgcgcccgg caccgacatc aaggggctgg ccatcagcgc cgtgctcgcg cagaccatcg
      121 gggccactgc cggaccatgg ctggggttgt gcgtcgccgg gctcggcgtg tggatcctgt
      181 tcaagaccca gctcgattgc gtggaaggca tgacgcgttc cattaccgac atcctgtgga
      241 ccggcagcgc gcgcgttcgc cgctggcgcg gcggcgatgt acgcgcggtg tactacacgg
      301 tgctggccgt cgtggtggtc tgggggatca tcgcgctcaa gctcgcggcg ccagtaatgc
      361 tgctgcagat tggcgcgaac atcgccagtg cgatcttcgt gatcgcgtca ctgcacgtga
      421 tgtacctgaa cacgcgcatc ctgccgatcg cgctccggcc gcccctgtgg cgccgcctcg
      481 cgctcgtggg catggcgctg ttctacgggt tcttcgtggt gctggtaacc cggtcgctgt
      541 tgtagacctc aacgtttgcg gctgatctcg attccgttgc cgacttcgat cagcacagaa
      601 tccatgtctc ccgcggcgcg cacgcgctgg cgataggcat cggcgtgcgg acgcgcgctc
      661 tcggggagca gcatgttgtc ggcggcgatg atgccgccca tggcgagctt ggggtgcacg
      721 agctcgaagc acggcaggta cagatccttc cacacgtcga gcagcacgaa atcgaaaggg
      781 ccgggcaggg agcgcagcga gtccaggcag tcgccggcat agaactcgac gcggctcgac
      841 aggccggcgc gcgtgagcgc ctggcgcgcg tgctcgatct tgttttcctt gagctcgagc
      901 gactggacct ttccgcccgt ggttcgcgcc gcggcgccca gccacacggt ggaatacccg
      961 tacgaggagc cgagctcgag gatgcgcttc gagtgcgccc ccgtcgccag tagatagaga
     1021 aggatgccgg cttcgcgtcc cactggcagc agcaggtcgt cgcgattggt gccgggcgcc
     1081 gagaagcggc gctgttcttc ttcggcgcgg cgctcgaatt cggcgagcac gcgttcgatg
     1141 acaggatcga ctagaggcat gcgggatctt ttgggttcgc gggccttggg cctcgacggc
     1201 cggcatagtg catggttgtc cgcgggttgg cgagtgccac cagaaaactt tgctagccag
     1261 tgatcggaca tgaaatttcc gcatggatcc ccggggtttg taggacgtcg tgaagccgcc
     1321 accacatatt ccgtgcgacg ttgcactggc gtacgcccca aacgcgtgct agccggcgac
     1381 ggacctcggc tcccgggccg ccgttgcgtt gctgcggaaa cgcccgcact atcgccgtcc
     1441 tgatgacggt ccctggtgat ttcacgttcg actttcgtcg taccgggccg cgccgacgcg
     1501 ccggccagac caaaacgcaa ggaatgaaac atgcgcaatg cgctcgtcag agtagttagt
     1561 gttctcggcc tggggtgcat cgcgccgggc acggcaattg ccgccgacaa tgatgggctt
     1621 gcgttgccgc ccggattttc cgcggtggtg gtacacgagg gccagggcac ggcccgccat
     1681 ctcgacgtgg ccgcgaacgg ggatatttac gtagccggcc gcaacggact ctccgcgatg
     1741 cgcgacacca atcgcgacgg caaggccgat atcgtcgcga ctttcggcga tgtgaagggc
     1801 acggaactac gcctgttcag gaactggctg tacgtgtccg acgacgtcgg cgtgtaccgc
     1861 tacgcgctcg ccaaggccca gcttgccccc gcgggttcac gcgaaaccgt ggtcagcgaa
     1921 tttccgaagg agcggcagca ttcggacaag actttcgcgc tcgatgcgaa gggcactctc
     1981 tatatcaacg tcggtgcgcc ttcgaactcc tgccaggaga aggaccggca ggaaggctca
     2041 ctgggattga acccctgtcc catcctcgag aagtacggcg gtgtgtgggt gttcgacggg
     2101 acgaagctga accagactcc cgcgaacggc cggcgcttcg cgaccggcat gcgcaatgcc
     2161 gtggccatcg agtggaacgc cacgcagaac tcactgttcg ccgtgatcca cggccgcgac
     2221 tcgctggaca cgctattccc tacgctctac accgcggaag acaacgccac gcgccaggcc
     2281 gaggagttcc accagatcgt cgatggggga gactacggct ggccatacac cttcttcgac
     2341 accaggctgg ccaggcgcgt actcgcgccg gagtacggcg gcgacaagac gaaagagccc
     2401 gaagccggaa aatatccgga gccgctggtt gcgtttcccg cgcactgggc gcctaacgac
     2461 ctgctgttct attcgggcaa gagcttcccg gcgaaatacc gcgaaggcgc gttcattgcg
     2521 ttccatggca gctggaaccg cgcccccgag ccacaggccg gttacaaagt ggtgttccag
     2581 cctatgaagg atggcaaggc taacggcccc tatgaagtct tcgcagatgg attcgcgggc
     2641 gatatgaagg acaacagtcc gcgaaatgcg caataccgtc ccgtcggtct ggcggtgggg
     2701 ccggatgggt cgctcttcat tgccgactcg cagaaaggtc gcgtgtggcg gatctcgtac
     2761 cgcggaaaat gagcgcgcga tcgctacgcc tggcggcggt gttcgctgga ctgtggcttg
     2821 ctgccgccag ttcggtcgcg aatgagcctg cgcctggcca gttgctctac aacaagcatt
     2881 gcctcgcctg ccatcaggcc gatggtggtg gcgtgcctaa catgcagccg gccattgccg
     2941 gtggaacctg ggtgaagggc gatgcgcgcg cgctggcgtt gttcgtgatg tcgggcggct
     3001 tcgattccgc gtcgcgcaag gacagcgacg tcggcaacgt gatgccgccc ttccaccagc
     3061 taccggatgc ggacctcgcc gccatcctca ccttcatccg ccagaagttc ggcgacggtg
     3121 gctcgccggt gagcgccgcc gacgtggccg atgctcgtac cagcttgccg gcgagcccaa
     3181 aatagcctgg gggccgaccc caggcttttt ttacacgctc atttcacctt gtagaggtgc
     3241 acgtagcggt cggtcttgcc gcgaactgcc gcatcgaaca ccagcttgtc gagctgatcg
     3301 tccttgttgc gcaaaccgtc aaaggttttc tcgagcacga agccgtggga agtcaggctc
     3361 ttttttgcga actcctcgtt gagccgatgc gccttgctgg ccatttcggc gccggtgccg
     3421 gcgggcgcgt tgtgatcgac gatcagtagt tttccgcctg gcttcaacat gtgtttcatc
     3481 gattcgatga agccatcggt gtcgatgtcc ggccagcctt ccttctcgtc gacccagaaa
     3541 gcgtcgtgat aggacatcac gatgacgatg aggtcgacgc tcttcggcgg gaagtggatg
     3601 gagctgacct ccaccaggcg gcgttccacg ttcttgaggc gtccttcggt gaagcgcgcc
     3661 ttgaccgcat ccttggaata ctgctcgtag ggcacgttgt tgaccgaaag taccttgccg
     3721 ctgggaccga ccacgccggc tagcaactcg gtgtagtagc caccgccggc gaacatgtcg
     3781 accacggtca tgccgggttt caccccggca aaggccagga cctcggccgg tttttcacgc
     3841 gcatcgcgct cgcgatcctt gtcggtgcgt tcgggactgg cgatggcgga ctcgatcggt
     3901 gtggcgtccg cggcgaacac gggcgcggcg agacagagca aggcacttgc gagcagagtt
     3961 ttcatgaggt tctccttgcg tgcattatgc gaatgccggg gcaggcaatg gaaccacgtc
     4021 catcgcgtcg gttactcatt ctggcgaacc gcaagccatt ctttcggggg ctcacatgcg
     4081 caattcattg agattgatca ctttcactct gttgctggcc acgacctgta ccgctttcgg
     4141 ggcgggcgga ggcgaattgc cgaatcgatc cagcggctcc gcatcgcgtc tcgacaaacc
     4201 cgtgacgccc gaggaccagg cgcgcaacga ctacaacgcc ggtgtacgcc tggtcgaaaa
     4261 agcggacgag cttgccgctg acgcattgca tcaggccgac gaacgcaaga aggctaaagc
     4321 cctgtccaag gcggagcaga actacaacac ggcgatgaag aaattctcca acgccacgca
     4381 gcttcaacca tcgaactacc aggcctggaa ctacatcggt tataccaacc gcaaggtcgg
     4441 caagttcgat gccgcgctcg cggcctacga tcgcgcgctc aaattgaaac cggactacgc
     4501 cgaagccatc gaataccgcg gccacgcgta tctcggactc aatcgcctcg ccgaggcaaa
     4561 ggcggcttac cttgcgttgt ttgccggcaa tcgccagctc gccgccacgc tgcttgctgc
     4621 catgcaggaa tgggttggca aacaccgtgg aagcgccgag ggcgtggacg gcgccactct
     4681 cgattcgttt gcatcgtggg tgaatgagcg cagcaccatc gcgagccaga ccgcggcgct
     4741 cacgcgcgaa ggcgccgcat ccgcctggca ttgatgaacg gcgtcgcagg gcgagtcgcc
     4801 gccattgccg tcaccacggt tctggcggtc atggtgacaa ccacggcgtt cgcaggtgag
     4861 ttccgctggc aattgcccgc gggattcccc gagcccgcgg tcccggccga caaccccatg
     4921 agtgcggcga aggtagacct gggcgcgaag ttgtttgccg acccgcgcct ctcgatcacc
     4981 ggccagtatt cctgccggag ctgccattcg cccgagcgcg cattcaccga tggccttgcg
     5041 cactcgcgcg gcgccaccgg cgaggagctg ctgctcaatg cacccacact gctcaatgtc
     5101 gcctacaact cttcgctggg ctggcgcgac gcgaacctgc ggacgctcga acggcagatg
     5161 cacggcccgc tgttcaacaa ggatccgcgc gagctcggcc tcgcggggcg cgaggccctc
     5221 gtcgaaaatg cgctgctggc ggaccaggag atggtgcgcg cctttggcgc cgcgttcccc
     5281 gggatggacg agccggtgag catggataac gtgattcgcg ccattgcggc ctacgaacgc
     5341 acgctttttg cgggttactc cgctttcgac cgctatgttt tcggcggtga ccatgacgcg
     5401 ctcagcgaac ggcagaaggc cggcatgcag ctgttctttt ccgggcgtac gggctgtgcg
     5461 gcatgccatg gtggcgtgaa tttcgccggc gcctgggtgg atcgcgacca cccccaggcc
     5521 gagcccgtct tcgccgacac cggtacgggc gaggcagtgc gtgtgccgac gttgcgcaat
     5581 ctgccggcga cggcgcccta catgcacgac ggccggttcg ccaatctcga ccaggtgctc
     5641 gatcactatg agagcctggc cgtcgacccg gccgcggatc cgcgcctgcg gcgcaaccca
     5701 ctaactacca gcgagcgtga gagcctgcgg gagttcctcc tctctctggc ggacgggctg
     5761 gcggacagtc gatagcgtat gcttgcgcgc cagcatgcac ccggacctca ccctcggccg
     5821 tcacattctg ctgccgcacc cggtttccgt gccaggcgcg ttgcaggatg tggtgcatga
     5881 gctttcggta ctcgtcgcgc gtccagaggc cgaccagctc aacctgacct atcgcctctc
     5941 gggtgacctc gatgcactgc aactgccgga gccaaggccg ccggtgcgaa ccgacgggtt
     6001 gtggcggcgc agttgcttcg aagctttcat cgcgcacgcc ggcgggcccg actactggga
     6061 atataacttc tcgccttcgg gcgcctgggc ggcctatcac ttcagcgcct atcgcgaagg
     6121 aatgtccccg ctgctgaagg gcgccgcacc cgcggtcacg ccgcgcgtcc gcaccgactc
     6181 cgttgagctt tccgtgcggc tcgatctttc atggctcgtg cgtggaggag gagtgggact
     6241 gcgtctcggt ctggcctcgg ttgtcgaaga caaagcgcga gtgctgtcat actgggcgct
     6301 caaacatccc gcggagaaac ccgatttcca tcactcagac agctttgtgg tcgagctcga
     6361 ttgatcttcg acaggctatt cgcctgaagg cccgctcgtg atccagttcg gcatcgaccg
     6421 cctgttgggc gacttgaaac tgcagcgcga gctagaaggc cggcgcgttg cgctgctggc
     6481 gcatccggct tccatgaccg cggatttcga acattcgctc gatgctttgc gcgccattcg
     6541 gggtctcgac atcaccgcgg ctttcggtcc gcagcatggg ctgcgaggcg acaagcagga
     6601 caacatgatc gagtcaccgg atttccgcga tccggtgcat ggcattccgg tattcagcct
     6661 gtacggcgaa gtgcgccgcc ccaccgaaga catgctcgac catttcgacg tgttgctggt
     6721 ggacctgcag gacgtcggct gccgcatcta tacgttcatc accactcttc tatatgtgct
     6781 ggaagcagcc gcgaagcacg gcaagacggt ctgggtactc gaccggccca atccggcggg
     6841 ccgtccggtc gaaggtttga gcctgcgcca ggggtgggag agcttcgtgg gcgcgggccc
     6901 gattccgatg cgccatggcc tcacgctcgg cgaactgggt atgtggttcg tgagccattt
     6961 tcacctcgcg gtggactatc gagtcattcc gatgcgcggg tggaagcccg acgaggagcc
     7021 gggtttcggc tggccgctcg gagggcgcaa ttgggtgaac cccagcccga acgcggcgaa
     7081 tctttccatg gtgcgcgcgt atgcgggcac ggtgatgctc gagggaacca ctctgtcaga
     7141 ggggcgaggc accacgcggc cgctggagat gttgggtgcc cccggtctgg atccgcgatc
     7201 gctgctgacg atgatgagcc gcatcatgcc ggcctggatg accggctgcc gtctgcggcc
     7261 gtgctggttc gagccgacgt tccagaaaca ctccggcaaa ttgtgctcgg gcttccagat
     7321 tcacgtcgaa gatggcgcgt atgaacatgg cgagttccgg ccctggcgcc tgatcgccgg
     7381 cgctttcaag tgcatccggc gactgcgggg cgaatacccg ctgtggcgtg attttcccta
     7441 cgagtacgag cactcgcgcc tggcaatcga ccttatcaac ggcagcgaat tgctgcgcga
     7501 gtgggtcgac gatccggcgg cgacaccggc ggatctcgat cgtctggccg cggttgacga
     7561 ggcggcttgg gaagaacagc gagcagacct cctgctgtac cgctgatctc gcgcggccga
     7621 cgatctttcc cggggagcat caacccaggt cgcgagcgga agactcgtgc accgacgtca
     7681 acatggggcg gttgatgcgc tgcacggcat cggcgagtgc gccctcgaca cattcctgag
     7741 ccttggtgcg ctgatcgaga ttcagcgcgt tgcccggggc gagcccgcag accttgcgcg
     7801 cagccatctt gagcttgcga tacacgttcg cggctccctc cggtgtggcg aaggcggtgt
     7861 cctggtaggt cacgctgatg cgcggcgggc tgtccgtcgt ttcagcggcg cgcacctcga
     7921 cggtgagcag catgagaaag gtgaccgcga tcgcggcggc gcctgcggtc aggtgctcgg
     7981 aagtgcggcg cagcacagag gcggaattca ggcgagtcat ggctaatcct ccggttaaaa
     8041 gagcggaaaa cctgcgatga cgcgtggcga ctcatcgctt gccggcgtga gaggcgagtt
     8101 cgaaacgtgt caatcagtaa cgctgatata gacgcgcgac gaccggaaac ggttgcaagg
     8161 aaaattggcg gattgcgctg aattgttgag cgcccgcgcc gaacggaaaa gtgtccgcga
     8221 tgcggacact ttggaagtgg tttaaaccgg ccgatcagcc agccgaacta ctcggcggat
     8281 tcggctccgg cggccacgaa ggcgaggtcg ctcatgttca ccttgcccag gtcgaacttc
     8341 ttcgcggctg cggacatgcg cagaagcgcc tgcgcgatgg gcatgacggg cttcttgtcg
     8401 cccggcttca ccttgtggaa acgaaccggg tttattcgcg ccaccacgta actgcgcaga
     8461 tagggcgact tcatgccgcg cgcctgcaac tctgcgacga tgcgcttcac ctcggcatct
     8521 atctcctgaa ggcgggccgc gtaggccgat cgctcgcgca gcgccgcggg caatggcctg
     8581 gtggtcagcc tatctacctt cttgagcacg gggctgtagg cgccgccgcc gaagcgcagg
     8641 ttttcttcgt agacgaggcc tagcgtgagc agctcggcgg cttcgaattc cgccgcaagc
     8701 gaggcttcgg tacgcgtggg ctctcgcttg gcgaggttgc gcgccatacg caccacttcg
     8761 aggctcttgt ccttgaggtt gtgagccttt tcggtgttca gcgcgaggat gcggtacgac
     8821 agcgtttcat ccggcgagat gagcacggtg atctgcttca ggcccagcac cttcgcggcg
     8881 gccaggcgat ggcggccgtt cggagtccag aagcgaccgt cttcgccacg caccacgatg
     8941 agcggatcga gaaatgccgc ggtggcttcg atggcggcgg ccagcttctt ggcgtgagtg
     9001 ggcgagaggt cgcgctgaaa tggagtgggc tgcacggcat tgaacggcag cgatgcgacc
     9061 agcaacggac ggccgcccag cggctcgcga taggcgccga tcggggcgcc gccggccttg
     9121 cgcaccaact caaccacggc ggcgatgtct tcgtgatcca gcgccaacgc gacctctgtg
     9181 gccgacaggc cgcgcttcac ggcattcggc gcgagcttcg gcttgcgctt tgccgacgac
     9241 ttagccgctt tctttttcgc cgccatgatc tctccccact tcgttgccga gaatgatgcg
     9301 cgccccgcgc gcgtacgtga aataggccca ggcccagttg atgagcacga tgagccggct
     9361 gcggaa
//
LOCUS       DASQJS010000434        21570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4677237, whole genome shotgun sequence.
ACCESSION   DASQJS010000434 DASQJS010000000
VERSION     DASQJS010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21570
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4677237"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VEW08_17055"
     CDS             <1..536
                     /locus_tag="VEW08_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42502.1"
                     /translation="AGAFDERIKLTIPQEPGSGGSGTWRVSQSILSQGQSTQTLGQIV
                     GENVWFRQNFSQFSNTVPKLPFDHHSIEGLVAPRALLVIENNILWLGPQSSWTGANAG
                     HRIWEALGIPDRMGYSLTTEHGHCSFPSSQQAEVNAYVQKFLVGGGAGNTNVMRNDPG
                     VPFNQAMWINWTTPALQ"
     gene            629..1069
                     /locus_tag="VEW08_17060"
     CDS             629..1069
                     /locus_tag="VEW08_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42503.1"
                     /translation="MVRLLLVFLFLPLTASAADEGRFSFLEQEVRNLQRQVQVLSRQV
                     DELRLQPGRLEPKSLVPAGTAPGAGSAANLPRWVDATLWRRVRPGMSEIEVIGTLGPP
                     TSMREEEGARVLLYAMEIGSTGFLGGSVKLRQRAVVEILQPTLH"
     gene            1166..3043
                     /locus_tag="VEW08_17065"
     CDS             1166..3043
                     /locus_tag="VEW08_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42504.1"
                     /translation="MNTFSRIGSALARLAVFSCALAFTACGGIGDGSSAMPAANAPPT
                     SSCTSDCGTVLIGMTDADGDFLSYTVDVVSLKLKRANGAVVETLPATTRIDFAQFVDL
                     TELFTAATVPSGAYVAATLRLDFTNADISVEMGGAPVAASAVDAAGNALGVVDVNVTL
                     DNRHHLVVARGLTSVFTLDFNLAATNSVDLTTTPVTVTVTPALVASLEHVDQKDLRVR
                     GPLVSVDTAAGTYVIDVRPCNMRDRHYGQVTVHTDADTTFEVNGTTYTGSAGLAALSS
                     AGAGTLTTAFGTLTKSTHQFDAQSVRAGTSVSGAGIDTVLGQVLSRSGDVLTVRGATI
                     NRNRDGARFARGTVLVTIGPETKVSKDGADAAPGDISVGQAIEAFGAATPVDSSAMSG
                     DWTMDATAGRVRMHATPIYGFVKQSSTGAVTLQLDSIAGRRVSAFNFAGTGTSAAQDA
                     DPANYEVSTGALDLSGLSNGEPTKLVGYPTAFGAAPPDFDARTLVDYPRLPALLSMSW
                     GIEGTKAPFTSQEPTGLVLDLANPSIGRLHFLSIGPRVLDLKSLPASPSIVPPSDERT
                     AYLVVMRGESRSFQDFADFVAELGTRLDGDTAMVGFTATGSYDGATNVVTARSIVVLL
                     K"
     gene            3128..5434
                     /locus_tag="VEW08_17070"
     CDS             3128..5434
                     /locus_tag="VEW08_17070"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HYJ42505.1"
                     /translation="MVKPSIGSALAAMLVAACFAPGAKAGDASGWITHPESGARTPVV
                     LQFRRELTLESVPAKFQVTVTADNRFVLYVNGDRVASGPSTGTVESWRYATVDLAPHI
                     RNGDNVITATVWNFGDVAPAFQQTVATGFRLIGDPVSTSVPGWRVRIDTGHSAVSGKE
                     QIPWQYYVASAPEVIDARKTPSEWQDAVPAPEAAVRTLIADPFPPQLFAATAPGQVVR
                     SSITGGDRFPSRPLVIPANTTAKLLIRRDAMISAYPEIEVQGGRDATIRLQYGEALYD
                     AAGRKDDRNLIGDRQMRGFHDTFIADGRPQVFAPLWWRTFRFVEIEVVTQAEPLTLRA
                     FRLHETGYPFQQVASFVSSDADLNRIWDIGWRTMRVDAHETFMDSSYWEQLQYTGDTR
                     LEMLITYAVSGDARPARQAIEAFAESDTEGGLMQGAYPSRLSNVIATFSFAWVGMLAD
                     WSVEQPDRALIVRHLPRMRRVLAWFEPLLNEQGLLRKNPQWNFIDWSGQKWDDRDIFP
                     SWGAQNGSCLMTAMWLGALRQGDALERRHGDGTRAAEFRAKADRARSAIRKHCWVQER
                     GLFADDGDRAVFSQHMNVFAVLYDIATPEEAPVILDRITARGRGIEAPAGMYSPTYYF
                     AWYLVRAFEHAGLAERYFELLQSWRDLLAFNYTTWPETREQPRSDTHAWSAHPTADLL
                     RIVAGIRSGADGYARVRIEPMLGSLTSLDATAATPSGPVKVSYRIAGGKLIAIIDRPA
                     ALAGTFVWHGKEHPLNGVHTRLELALKH"
     gene            5492..6316
                     /locus_tag="VEW08_17075"
     CDS             5492..6316
                     /locus_tag="VEW08_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42506.1"
                     /translation="MRINIMGAALLWVAGVAGAQADDGNPLDSKVVVDLGGFFLSTDI
                     DVRLDGEDSNQVGDDIDYDDTFGIADFERFRLDALWRIKGKHSLRGTYFTNNRTASNE
                     ISRDINFGDETYPVGATVTARSELEVLQLSYDYVFKRRENYELAGGIGVHMLDVALGL
                     DATVAVGSGSAMRELTESGTTAAPLPTLGFRGVWRLPHNFYATALVQYFYIDFDDYVG
                     SLSDLKATLVWQATPHVGVGLGYNDFRFRFRLDKDRFDGRLGWDYGGAMAFVSITY"
     gene            complement(6326..7306)
                     /locus_tag="VEW08_17080"
     CDS             complement(6326..7306)
                     /locus_tag="VEW08_17080"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HYJ42507.1"
                     /translation="MKRRTLLSAGAGAMILAPHVKPSLAASEPDRAKELYRLLGKLPP
                     RDRKVGAELVSTEDRGTYTLEKLSLDLNGIERAPAYFARPNGKTGRLPTVLFNHSHGG
                     GYKIGKTEFIEGREYLANPPYAEFLTSLGYNALCFDAWIFGERATRTELETFKDMLWH
                     GQVLWGMMVYDSLKAVDYLVTRPDVDAKRLGTVGMSMGSSTAQWAGALDPRLKVVVDI
                     CCLTDWHTLVEVDGLKGHGIYYYVPDLLSHFTTTQMNALIAPRAHLSLAGNQDKLTPR
                     AGLEKVDRELKGDYAAAGHPENWKLLRYDGGHLENPQMREEIRKWFVEKL"
     gene            complement(7332..8816)
                     /locus_tag="VEW08_17085"
     CDS             complement(7332..8816)
                     /locus_tag="VEW08_17085"
                     /inference="COORDINATES: protein motif:HMM:NF021041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HYJ42508.1"
                     /translation="MSTANKIAMLLAASLVCAFASAAEPARADVVAAMKKAATFMNEK
                     VALRGGHVWLVSEDLGRRWGEIPARPSQIWLQGGTERVGQVMLDAYEVTQDVYYLDSA
                     RKAADALVFGQHPLGGWHYFIDFDPAGTPGWYETDASRFRWGYEEYRHYYGNATFDDQ
                     VTPDAAMFLLRFYRLTHEAAYREPVLKALNFVLAAQYPNGAWPQRFPLRYEFAHDGLP
                     DYTSFYTLNDGAMFAIVELLYDAYETLGDPRYFESVRRGMDALLALQGPDDQAAWAEQ
                     HGPNMRPIAARTHEPAGYVVRESAMSIHMLEEFYLRTGDARYLAPVPHCLDWFDRINR
                     EAAAAKYPTPRYWEPGTNKPVYVVRTQELTAEGYGKYIWTTDPAKARCDGQPCKVDGK
                     PIVDVAPMRARNAALAALATPKARAARLAEMKTRRSRAPRGNENIADVIAALDARGAW
                     VTDGNVVQRANAPTEAEQREIVRGINTGIFASRMETLIAYLRKP"
     gene            complement(8834..9361)
                     /locus_tag="VEW08_17090"
     CDS             complement(8834..9361)
                     /locus_tag="VEW08_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42509.1"
                     /translation="MFNIRTAVFVASLFTMSNAVAQSSSPPTDPAVTPPTAQMPVYDT
                     ALAPGWQNWSWAKAELSVEIPGSARKPISVVAGPYQALYLHHDPFSTAGMTKLSLLIQ
                     GSASGGEVRILALIDGKTVGEGKLVKLGNIGWTRVVTPLATLGVEDKTIDGIWVQNAT
                     DAELPKFYVTEIKID"
     gene            9496..10302
                     /locus_tag="VEW08_17095"
     CDS             9496..10302
                     /locus_tag="VEW08_17095"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HYJ42510.1"
                     /translation="MKIALIAALALGLAAEAQSQPVNPAGDVKAPAPDEWHSLFNGRD
                     LSGWTIKINKHPLGENYADTFRVEDGVIKVSYDGYGKFDEQFGHLYSNQAYQNYVLRL
                     EYRITGTAVADSPPWAKLNSGVMIHSQSPLTMGLNQAWPASMEVQFLAVGATAGRQTG
                     NVCTPGTNLEMKGKLVTDHIIDSTSKLFPVDEWVQVEVEVRGNKTVVHRINGVEVLRY
                     QHPQLDPKEESAQALLAAGAPLQLSFGHIALQAESQPVWFRNIRIRQLAR"
     gene            10299..11306
                     /gene="epmB"
                     /locus_tag="VEW08_17100"
     CDS             10299..11306
                     /gene="epmB"
                     /locus_tag="VEW08_17100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HYJ42511.1"
                     /translation="MTFAPSTWQRELSRAITDPVQLLAELELDPALAAPARAASSAFA
                     LRVTRSYLARMRRGDVDDPLLRQVLPLAAELIESPGYGSDPLHEHAATRAPSLLQKYA
                     GRALLITTQACAIHCRYCFRREYPYAEQREAEEGAGRWSAALDVIANDTSIEEVILSG
                     GDPLSMSDARLARLTDAIAGMPHVQRVRVHTRQPIVLPSRVDAGLLAWLGGVRQPVVF
                     VLHTNHANEIDDEVRAACERLRRGGATLLNQSVLLKGVNDDVDTLAQLSRALMDAGVV
                     PYYLHLPDRVRGTAHFDVAEADAQRLIERLSTRLSGYLVPRLVREVPGAAYKVPSVIT
                     P"
     gene            11400..11930
                     /locus_tag="VEW08_17105"
     CDS             11400..11930
                     /locus_tag="VEW08_17105"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYJ42512.1"
                     /translation="MDEALIRRAQGGDATSFAELLDLHYDTIYRFAWKWCGHAANAED
                     IAQLACMKLATSLAQYRFQAAFTSWLYRLVISCAQDWQRSQQRHDHDELPDEEAMTES
                     PRHEDGIYLAQVLAQLAKLGEGMRETALLVHAEGMSHAEAGAVLGVSESTISWRVHTI
                     RKHMGQPAARASLAYE"
     gene            11923..14280
                     /locus_tag="VEW08_17110"
     CDS             11923..14280
                     /locus_tag="VEW08_17110"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HYJ42513.1"
                     /translation="MNEKSFESKLARMSAPAPDPEARLRAKRAALEEFARVQAANQAS
                     ATSTSASDSKGLWDALRLFRDDSSHGSTSMAWFVRRNLFAGVAGACVVGLGIAIVWPF
                     FSHYGDSRLFDDVRQPEVTVDLKPAAKEDPAAPPVSKSPEAKPAPAQPEPTQPEPAKP
                     ESRRNQAAVSAALEKDKELNEILVTGERARLQAALETKREAVTAVDSIVAEDIGLPPD
                     PNVAEALQRIPGVQITAGAQPAPQQVDEEIQEVITTGVRGSRRAQPAHKGMAPATPGS
                     SGRVAANAYAPPASPPPMVTMGVMDAYELSKPVVDEGRDLFEKFAVNPVKQVADEPVS
                     TFSADVDTASYSFVRRQLNSGRLPQKDSVRVEEMVNYFDYAWPAANSAKAPFKPTVVV
                     SDSPWGKGRKLVHIGIKGYEVSRDDAPGANLVLLLDVSGSMDSPDKLPLAVQSMELLL
                     SSLKPTDTVGIVVYAGAAGTVLEPTPVKNKQKIIAALRDLRPGGSTAGAEGIERAYQL
                     AEAHFRKGGVNRILLATDGDFNVGIAGTNELKGFVERKREKGIFLSVLGFGQGNYRDE
                     LAQALAQNGNGVAAYIDTLGEAQKVLVQEAGASLFTIAKDVKLQVEFNPATVAEYRLV
                     GYETRALKREDFNNDAVDAGDVGSGHTVTAIYEITPVGSDTRMVDKSRYAKEPQPPVA
                     TGTASEYGFLKIRYKLPDSDTSELIQQPILIAPSDLPQNLLRDVQFSTAVAGFGQLLR
                     GSPFTGPLKYDDIIKQAQAAKGDDPFGYRTEFVQLVRKAKVAREM"
     gene            complement(14285..15082)
                     /locus_tag="VEW08_17115"
     CDS             complement(14285..15082)
                     /locus_tag="VEW08_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42514.1"
                     /translation="MATNKILLSVLLTTAAASVHADVTVNTTTSGKAAFISLGGDGVT
                     QIKGTRQRSDQMVSGKAQSLILDIDGRRFVDLDAKGKKATVTPLAIIADELQKVGVGT
                     MNATLTKTAQTKQIAGFPCTVHDIRLSLPFSPTGQTGQGMDLNMVLAGTVCLSTSAPG
                     LADYQKFYKAAADSGFIFGDPRSAKTPTGAAQAKAYAELTRKMAEAGMALETHVTVDA
                     TGDNPMAGMFSKLAKSDITTMVTKIATGDLPADAFEIPAGFKVKTQK"
     gene            complement(15182..16540)
                     /locus_tag="VEW08_17120"
     CDS             complement(15182..16540)
                     /locus_tag="VEW08_17120"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYJ42515.1"
                     /translation="MNRRMSDDLTLFGVRRMMEGVIPPTLCTASLDRMPNVSYLSLAE
                     YVDPLHIALSFQFFNRSRENVLATKRAALTLDDPYTGAGVVLQLEYLRTETEGPVFER
                     LRAKLMGVASHVGMDQVFHLRGADIYRVLELRRVPGRLELPAAAPRVDLATNSRILSE
                     RLANCGELSDLLDTLLDGLGELLRIDNAMLWLFDSAQEVLTLLASRGYEVGGAGAEIS
                     TGDGIVGTAVREGVAIRVGHMMNMARYARAARDRADALGFEPVLKSEIPLPGLKEPRS
                     QLAVPLRARGRVLGALLVESKHDQHFGYDDEDALMLLCGQFAVAMNLMQPPEPEPPPR
                     EPPKASASPQAGPAVQLRRYSRDNSVFLDDVYLIRGVAGAILWKLVGEYLRSGRREFS
                     NRELRLAPELRLPDVQDNLEVRLLLLQRRLAEQNAPVQLEKAGRGRVRLNVARPVVME
                     SD"
     gene            complement(16537..17016)
                     /locus_tag="VEW08_17125"
     CDS             complement(16537..17016)
                     /locus_tag="VEW08_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42516.1"
                     /translation="MALTQEIIDLLGAGVSHQVGGCTKDGRPSLCRGLAATLEPDGRL
                     VVILSAETGFEVIDAVRDTGRIALNLTLPENYKSISLLGRDAVVSHGGAAFRELVLDR
                     YQKFRAQLDVHGIPPAYTEAWYLRPDEDLMAIRFTPFSARNQTPGPDAGSALALTKP"
     gene            17138..18094
                     /locus_tag="VEW08_17130"
     CDS             17138..18094
                     /locus_tag="VEW08_17130"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HYJ42517.1"
                     /translation="MSKPVITVFGATGAQGGGLARALLADPGRHFAVRAVTRKPDSAA
                     ALELERAGAEVALADLDDSCSVLRAMEGAYGAFLVTNFWEHQSAQKELIQAHNLAAAA
                     AQAGVRHAIWSTLEDTRDSIAVDGTRMPVLQHQYNVPHFDAKGAANRFFAQLRVPVTF
                     LYTSGHWENLIHFGMGPQRGPDGKLAVTFATADARIPWIAAEDIGISASEIFLRGDAL
                     IGESIGIAGDHLTGAELAAQLGTALGEPVHYNAVSPDVFRGFAFPGADEIGNMWQFKR
                     ETEIAYCARRDLSRARALHPAMADFGTWLAANKSRIPVAKAA"
     gene            18101..19150
                     /locus_tag="VEW08_17135"
     CDS             18101..19150
                     /locus_tag="VEW08_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42518.1"
                     /translation="MPRAILHGSLALLWLAVGCAHGIARASEATALPEHLRDTGLYAD
                     VSMSQLQPGVLPFSPQYPLWSDGATKRRWIKLPAGTSIDATQPDAWDFPRGTKLWKEF
                     SHAGRVETRFIERVVDGSWRYASYVWSADEQDAVLAPATGIRDLPVPSAPGARYSIPG
                     QDDCRACHEGAPVPVLGFSALQLSPDRDRQAPHADAADSALSRMDLRTLASRGLLVHL
                     PSELLVSPPRIAAANPTERAALGYLHANCGHCHNDEGPLAVLEMSLAQRVGTKESAGS
                     VLRSIASVPGQFRMAGGTARLAPGHPDASVIALRMRSREPLQQMPPLGTSEVDFEAMS
                     LIARWIESLPIHQPK"
     gene            19179..19739
                     /locus_tag="VEW08_17140"
     CDS             19179..19739
                     /locus_tag="VEW08_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42519.1"
                     /translation="MFVRIALVVAASFARAGEQPAPAAGSEALARGKYLVTVAGCNDC
                     HTPLKMTPEGPRPDMDRMLSGHPESLEMPPAPKMPEGPWQNAFAATNTAWSGPWGVSF
                     TANLTPDRETGLGKWTVRNFTETIRTGRHLGRGREILPPMPIPMYKNFTDEDLAAIFT
                     YLQSIPTVKNRVPDPRPPAALASVTK"
     gene            19854..20465
                     /locus_tag="VEW08_17145"
     CDS             19854..20465
                     /locus_tag="VEW08_17145"
                     /inference="COORDINATES: protein motif:HMM:NF027675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HYJ42520.1"
                     /translation="MEPRRRMQLYRTMHLWHWISAAVCFAALTLFTVTGITLNHASAI
                     SAEPAVSNGGAQVPEGLRGPLAAGGVESAEPPSDIAAWAEEAFKLSLRDATAEWSEEE
                     LYLSAPGPGRDAWVSIDRASGAAKFEKTDRGWLAYFNDLHKGRNTGITWMIFIDVVAA
                     AVLFFSLTGLVLLWIQARQRTSTWPLVGGGIALVTALMIFFAH"
     gene            20478..20987
                     /locus_tag="VEW08_17150"
     CDS             20478..20987
                     /locus_tag="VEW08_17150"
                     /inference="COORDINATES: protein motif:HMM:NF021540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HYJ42521.1"
                     /translation="MIRLTRIAPGMLLAFAGGASAADLSLSVEIPQLPVAEYHRPYVA
                     IWIEGADQAIAANLAVWYQVRGDHTKWLPDLRQWWRRGGRDLKVPVDGLTGATRPVGQ
                     HLLKFDAAKAPLTGLKPGQYTVVVEAVREVGGREAVRLPFEWPIKAAKRESASGSKEL
                     GAVALSLNP"
     gene            21002..>21570
                     /locus_tag="VEW08_17155"
     CDS             21002..>21570
                     /locus_tag="VEW08_17155"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HYJ42522.1"
                     /translation="MKNSIVSHSAKLAALLIVMAPATSEAARAWLLPSQTILARSGGW
                     VTVDAAMADDLFSFNQGAMQVDALVVSGPDGQPVAPQNPAKGRTRSSFDLELRQAGTY
                     RIAVAENMVMARWEEDGKPKRWRGAPAEMATGIPANAAKLEVEQTQRRVETFVTVGTP
                     TDVNPGAGTGIELKPVQHPNDLYARETAK"
BASE COUNT         3824 a         6851 c         7003 g         3892 t
ORIGIN      
        1 ctgccggcgc gttcgatgag cgtatcaagc tcaccatccc gcaggaaccc gggtccggtg
       61 gttcgggaac ctggcgcgtt tcgcaatcga tactttcaca gggacagagc acccagacgc
      121 tgggccagat cgtgggcgag aacgtctggt tcaggcagaa cttcagccag ttctcgaaca
      181 ccgtacccaa actaccgttc gaccatcact ccatcgaggg cctggtggcg cctcgtgccc
      241 tgctggtcat cgaaaacaac atcctgtggc tgggcccgca gagttcctgg acgggtgcaa
      301 acgcgggtca ccggatctgg gaagcactgg gaattcccga caggatgggt tactcgctga
      361 ccaccgaaca tggtcattgc agctttccgt catcgcagca ggctgaagtg aatgcatacg
      421 tgcagaaatt tctggtcggc ggcggcgcgg gaaacaccaa cgtcatgagg aatgatcccg
      481 gcgtgccgtt caaccaggcg atgtggatca actggacgac gcccgccttg cagtgaactc
      541 cttctttgat tgaaccaggg cggcgtggag gcttccaatc aaagggcgga acggcgtgtc
      601 cgttccgcct tttccaaagg agacttccat ggtccggttg cttctggtgt tcttgttcct
      661 gccgttgacg gcatcggccg ctgatgaagg ccggttctcg ttcctcgaac aggaagtgcg
      721 caacctgcag cggcaggtgc aggtgctgtc gcggcaagtg gatgagctgc gcctgcagcc
      781 cggccggctc gagccgaaat ctttggtgcc ggccgggaca gcgccgggcg ccgggtccgc
      841 ggcaaacctg ccgcgatggg tcgacgccac gttgtggcgg cgcgttcgtc ccggcatgag
      901 tgaaatcgag gtgatcggca cgcttgggcc gcccacttca atgcgcgaag aagagggcgc
      961 gcgtgtcttg ctctatgcca tggagatcgg gtccacaggg ttcctagggg gcagcgtcaa
     1021 actgcgccaa cgcgctgtag tcgagatcct tcagcccacg ctgcattgaa catgtcgccg
     1081 cccggcgacc tttgcttgcg cgtgtcaacc gcgcgcctca ttgcgcgttg cctctgctaa
     1141 cacctttttc gatggggaga atcccatgaa cactttttcc cggattggca gcgcactcgc
     1201 tcgcctcgcc gtcttctcct gcgcgctggc attcactgca tgcggcggca taggcgacgg
     1261 cagcagtgcc atgccggcgg caaatgcacc gccaacttcg agctgcacat cggattgcgg
     1321 cactgttctg attggtatga cggacgcgga cggcgacttc ctgagttaca ccgtcgacgt
     1381 cgtctctctc aaactcaagc gtgcgaatgg cgccgtggtc gaaacgctgc ccgcgacgac
     1441 gcgcatcgac ttcgcgcaat tcgtcgatct caccgagctc tttaccgcgg cgacggttcc
     1501 cagcggcgcc tacgtcgcgg cgactctgcg cctcgatttc accaacgcgg acatttcggt
     1561 tgaaatgggc ggcgcgccgg tcgctgccag cgcggtcgat gccgcgggca atgcgttggg
     1621 cgtggtcgac gtcaacgtga cgctcgacaa ccggcatcat ctcgtggtgg cgcgcggcct
     1681 gacatccgtg ttcacgctcg atttcaatct cgcggcgacc aactctgtcg acctcacgac
     1741 gacgccggtc acggtgaccg tcacacccgc gctggtggct tctctcgagc acgtcgacca
     1801 gaaagacttg cgcgtgcgag gcccgctggt gagcgtcgat accgctgcgg ggacatacgt
     1861 gatcgacgtc cggccgtgca acatgcgcga caggcactac ggacaggtga ccgtccacac
     1921 cgatgcagac accacgttcg aagtaaacgg caccacgtac accgggagcg cggggctcgc
     1981 cgcgctgagc tcggcgggcg ccgggaccct caccacggca ttcggcacgc tgacgaagtc
     2041 gacacaccag tttgacgcgc agtctgtgcg tgccggcacc agcgtgtccg gcgccggcat
     2101 cgacacggtc ctgggacagg tgctcagccg cagcggcgat gtactcacgg tgcgtggcgc
     2161 cacgataaat cgcaaccgcg acggcgcgcg tttcgcgcgc ggcaccgtgt tggtgacgat
     2221 cggcccggag accaaagtgt cgaaggacgg cgcggacgca gcccccggtg atatctcggt
     2281 cggtcaggcg atcgaagcct tcggtgcggc aacacccgtg gacagctccg cgatgtccgg
     2341 cgactggacg atggatgcga ccgcgggccg cgtgcgcatg catgccacac cgatctacgg
     2401 tttcgtgaag caatcgtcca ccggggcggt cacgctgcag ctcgactcga tcgcgggccg
     2461 cagagtgtcg gcgttcaatt ttgccggcac cggcacgagc gccgcgcagg atgcggatcc
     2521 ggctaactac gaggtctcaa cgggtgcgct cgatctctcg ggactgagca acggcgagcc
     2581 gacgaaactc gtgggttatc cgacggcttt cggcgccgca ccgccggatt tcgatgcgcg
     2641 cacgctggtc gactaccccc ggctgccggc gctgctgtcg atgagctggg gcatcgaggg
     2701 cacgaaggcg ccgttcacca gccaggaacc cacgggcctg gtgctcgacc ttgccaaccc
     2761 gagcatcggc cgcctgcact tcctgagcat cggcccacgg gtgctggacc tcaagtcgtt
     2821 gccggcgtcc ccgagcatcg tgccgccgtc cgatgagcgc acggcgtacc tggtcgtcat
     2881 gcgcggcgag tcgcgctcgt tccaggattt cgccgacttc gtggccgagc ttggcacacg
     2941 cctcgatggc gatactgcga tggtcgggtt cacggcgacg ggctcctatg acggggccac
     3001 caacgtggtt accgcacgca gcatcgtcgt tctgctgaaa tagactgctg aagcaacgaa
     3061 tggccggtcc gcggatcaca ccgcgggccg gtcgcggttc gcgcgatcgg tgtgcgaaca
     3121 tcgcgcgatg gtcaagccat cgatcggtag cgcactcgct gccatgctcg tcgccgcatg
     3181 cttcgcgccg ggcgccaaag ccggcgatgc gtcaggctgg atcacccatc ccgaatccgg
     3241 cgcgcgcact cccgtcgtgc tgcagttccg ccgcgagctc acgctggaga gcgttccggc
     3301 aaagtttcag gtgaccgtga cggctgacaa ccgcttcgtc ctctacgtga atggcgatcg
     3361 cgtcgccagc ggaccctcga cgggcacagt cgagagctgg cgatatgcca ccgtggatct
     3421 cgcgccgcat attcgcaatg gcgacaacgt gattacggcc accgtctgga acttcggcga
     3481 tgttgcaccc gcattccagc agaccgtggc gaccggattc cggctgatcg gcgatccggt
     3541 gtcgacgagt gtgccgggct ggcgcgtgcg catcgatacc ggtcactccg ccgtcagtgg
     3601 caaggaacag attccctggc agtactacgt ggcaagtgcg cccgaggtca tcgatgcgcg
     3661 caagacgccg tcggagtggc aggacgcggt gcctgcgcca gaggctgcgg tgcggacttt
     3721 gatcgcggat ccgttcccgc cgcagctgtt cgccgctacc gcgccaggcc aggttgtgcg
     3781 cagctcgatt accggtggcg atcgattccc ctcacgaccg cttgtcattc cggcgaacac
     3841 cacggccaaa ctactgattc gccgtgatgc catgatttcg gcttatccgg aaatcgaggt
     3901 gcagggcggc cgggatgcga cgatcaggct tcagtacggc gaggcgctgt acgacgcggc
     3961 tggacggaaa gacgaccgca atctcatcgg cgaccggcag atgcgcgggt tccacgacac
     4021 tttcattgcg gatggaaggc cacaggtgtt cgcgccgctg tggtggcgca cgtttcggtt
     4081 cgttgaaatc gaagtcgtga cgcaggcgga gccgctcacg ctgcgcgcgt ttcgcctgca
     4141 cgagaccggc tacccgtttc aacaggtagc cagcttcgtg agcagcgatg ccgatctgaa
     4201 tcgcatctgg gatatcggct ggcgcacgat gcgcgtcgac gcgcatgaaa ccttcatgga
     4261 cagctcgtac tgggagcagc tgcagtacac cggcgacacg cggctggaga tgctgatcac
     4321 ctacgccgtg tcgggtgacg cgcggccggc gcggcaggcc atcgaggcgt ttgcggaatc
     4381 cgataccgaa ggcgggctca tgcagggcgc ctatccgtcc cgcttgtcca atgtcatcgc
     4441 cacgttctct ttcgcctggg tgggcatgct cgcggactgg tcggtcgagc agccggatcg
     4501 cgcgctcatc gtgcgccacc tgccgcgcat gcgccgcgtg ctggcgtggt tcgagccgct
     4561 gctcaacgaa caaggcctgc tccgcaagaa cccacagtgg aacttcatcg actggtcggg
     4621 gcagaaatgg gatgaccggg acatctttcc ttcatggggg gctcagaacg gcagctgcct
     4681 gatgacggcg atgtggctgg gcgcgctgcg gcagggcgac gcgctggagc gccgtcacgg
     4741 cgatggaacg cgagccgcgg aattccgcgc gaaggcggac cgagcgcgca gcgccatccg
     4801 taaacactgc tgggtccagg agagagggct cttcgccgac gatggcgatc gcgcggtctt
     4861 cagccagcac atgaacgtgt tcgccgtgct ctacgacatc gcgacgcccg aggaggcgcc
     4921 ggtcattctc gatcgcatca ccgcacgcgg ccgcggcatc gaagcgccgg ctggcatgta
     4981 cagcccgact tactactttg cgtggtacct ggtgcgcgcg ttcgaacatg ccggtctcgc
     5041 ggagcgctat ttcgagttgt tgcagagctg gcgcgacctg ctcgcgttca actacaccac
     5101 ctggccggaa acacgcgaac agccgcgctc ggatacccat gcctggagcg cgcatcccac
     5161 ggcggacctg ttgcgcatcg tggccggcat ccgctccggc gcggacgggt atgcgcgggt
     5221 gcgcatcgag ccaatgttag ggtcgctgac ctcgctcgac gccacggcgg cgacaccgag
     5281 cggcccggtg aaagtgagct accggatcgc cggcggaaag ctgatcgcca tcatcgatcg
     5341 gcccgcggcg cttgccggaa ctttcgtctg gcacggcaag gagcatccgc tgaacggagt
     5401 tcacacccgg ctcgagctgg cgctgaagca ctagcaactt ttgccgaagt tcttgctacg
     5461 ctcgccgcag gggtatctgg cgggaaatgg catgcgcata aacataatgg gggcggctct
     5521 cctgtgggtc gcgggcgtgg ctggggctca ggcggatgac ggcaatccac tcgactcaaa
     5581 ggtagtcgtg gatttgggcg ggttttttct gtcgacggac atcgacgttc gcctcgatgg
     5641 cgaggacagc aaccaggtcg gcgacgacat cgactacgac gacaccttcg gcatcgccga
     5701 cttcgaacgc ttccggctgg acgcgctgtg gcggatcaag ggcaagcatt cgctgcgcgg
     5761 cacgtacttc accaacaatc gcaccgcgag caacgagatt tcccgcgaca taaacttcgg
     5821 cgacgaaacc tacccggtcg gtgcgaccgt cactgcgcgc tcggaactcg aagtgctgca
     5881 gctttcgtat gactacgtgt tcaagcgcag ggaaaactac gagctcgccg ggggaatcgg
     5941 cgtgcacatg ctcgacgtcg ccctgggact ggatgccact gtcgccgtcg gcagtggttc
     6001 cgccatgcgc gaactgaccg agagcggcac cacggccgca ccgctgccga cgctgggttt
     6061 tcgtggcgtc tggcgcctgc cgcacaattt ctacgccacg gcactggtgc aatatttcta
     6121 catcgatttc gacgattacg tcggcagcct ttcggacctg aaagccacgc tcgtctggca
     6181 ggcgacgccg cacgtggggg tcggtctcgg ttacaacgat ttccgcttcc gattccggct
     6241 ggacaaggac cgcttcgatg gtcgactggg ctgggactac ggcggcgcga tggcgttcgt
     6301 atcgattacc tactgacgca tcgcctcaca acttttccac gaaccatttg cggatctcct
     6361 cgcgcatctg gggattctcg agatgtccgc cgtcgtagcg cagcagtttc cagttctccg
     6421 gatggccggc cgccgcgtaa tcgcctttta attcccgatc caccttctcg agccccgccc
     6481 gcggcgtgag cttgtcctgg ttcccggcga gcgacaggtg cgctcgcggt gcaatcagcg
     6541 cattcatctg cgtggtggtg aagtgactga gcagatcggg cacgtagtag tagatgccgt
     6601 ggcccttcag gccatccact tccaccagcg tgtgccagtc cgtcaggcaa cagatgtcga
     6661 cgaccacctt caggcgaggg tcgagcgcgc ccgcccactg cgcggtggag ctgcccatcg
     6721 acattcccac ggtgccgagg cgtttcgcgt cgacgtcggg ccgggtgacc aggtagtcca
     6781 cggccttcag ggagtcgtag accatcattc cccacagcac ctggccgtgc cagagcatgt
     6841 ccttgaaggt ctcgagctcg gtccgcgtgg cacgctcgcc gaagatccat gcatcgaaac
     6901 acagcgcgtt gtagccgagt gacgtgagga attctgcgta gggcggattg gccaggtatt
     6961 cgcgaccttc gatgaactca gtcttgccga tcttgtatcc accgccatgc gagtgattga
     7021 agagcaccgt aggcagccgg ccggtctttc cattgggtcg cgcgaagtac gcaggagcgc
     7081 gctcgatgcc gttgagatcg agcgagagtt tctcgagcgt gtatgtgccg cggtcttccg
     7141 tggaaacgag ctccgcgccg accttgcgat cacgcggtgg cagtttgccc aacaggcggt
     7201 acaactcttt ggcacggtcg ggttcggacg ccgcaagcga aggtttcacg tgcggcgcga
     7261 ggatcatcgc gccggctccc gcgctcagca gtgtgcgtct tttcatgatg ctcccgtcgt
     7321 tggtataggg ctcacggctt gcgcaggtac gcgatcagcg tctccatgcg gctggcaaaa
     7381 attccggtgt tgatgccgcg cacgatttcc cgttgttccg cttccgttgg tgcattggct
     7441 cgctgcacga catttccatc ggtgacccag gcgccacgcg cgtcgagcgc ggcaatgaca
     7501 tcggcgatat tttcattgcc tcgcggcgcg cgcgaacgcc gggttttcat ttcggcgagg
     7561 cgcgcagccc tggctttggg tgtggcgagc gcggcgagcg cggcattccg cgcgcgcatg
     7621 ggcgcgacat cgacgatggg tttgccatcg actttgcacg gctgaccatc gcagcgggct
     7681 ttcgccgggt cggtggtcca gatgtacttg ccgtaacctt cggctgtcag ctcttgcgta
     7741 cgaacgacgt acaccggttt gttggtgccg ggctcccagt accgcggcgt cggatacttc
     7801 gcggcggctg cctccctgtt gatgcgatcg aaccagtcga ggcagtgcgg caccggcgcg
     7861 agataacgcg catcgcccgt acgcagatag aactcttcga gcatgtggat ggacatggcg
     7921 ctttcgcgca cgacgtaacc ggcaggttca tgcgtacgcg cggcgatggg ccgcatgttg
     7981 ggtccatgct gctcggccca ggccgcctga tcatctgggc cctgcagtgc cagcaatgca
     8041 tccatgccgc gtcgcacgct ctcgaagtag cgcggatctc ccagcgtctc gtaggcgtca
     8101 taaagcagtt caacgatcgc gaacatcgcg ccgtcgttga gagtgtagaa agacgtgtag
     8161 tccggcaggc cgtcgtgagc gaattcgtaa cgcaacggaa aacgctgtgg ccacgcaccg
     8221 ttcggatact gcgcggccag cacgaaattc aacgccttga gcaccggctc gcggtacgcg
     8281 gcctcgtgag tcaggcgata aaaccggagc aggaacattg cggcgtccgg tgtcacctga
     8341 tcgtcgaatg tggcattgcc gtagtagtgg cgatattcct cgtaacccca gcggaatcgg
     8401 gaggcgtcgg tctcgtacca cccgggtgtg ccggccggat cgaaatcgat gaagtaatgc
     8461 cagccaccca gcgggtgctg gccgaagacc agcgcgtcgg cggccttgcg cgccgagtcg
     8521 aggtagtaga catcctgcgt gacttcatag gcgtcgagca tcacctgtcc cacgcgttcc
     8581 gtgccgccct gcagccagat ctgcgacggc cgcgcgggaa tttcgcccca gcggcggccc
     8641 aggtcttcgc tcaccagcca tacatggccg ccgcgcagcg cgaccttttc gttcatgaaa
     8701 gtggcggctt tcttcatggc cgcgaccacg tcggcacgcg cgggctccgc ggctgacgcg
     8761 aacgcgcata cgagcgatgc ggccagcaac atcgcgatct tgtttgctgt gctcatcgcc
     8821 ggattctacg gcgtcagtcg atcttgatct cggtgacgta gaacttcggc agctcggcgt
     8881 ccgtggcgtt ctgcacccag attccgtcga tggttttatc ttcgacgccg agcgtcgcga
     8941 gcggcgtcac gacacgggtc cagccgatgt tgccgagttt gacgagtttt ccttcaccga
     9001 cggtcttgcc gtcgatgagc gcaagaatcc ggacttcgcc cccagacgct gaaccctgaa
     9061 tcagcagtga gagcttcgtc attcccgccg tgctgaacgg gtcgtgatgc aggtacaacg
     9121 cctgatacgg accggccacc acgctaatgg gtttgcgtgc gctgcccggg atctcgacgc
     9181 tcaactcagc cttggcccag ctccagttct gccagcccgg cgccaacgcg gtgtcataga
     9241 ccggcatttg cgctgtgggc ggggtgacgg ccgggtcggt gggtggcgag ctggactgcg
     9301 cgacggcgtt gctcatggtg aacaaagacg cgacgaaaac agcggttcga atattgaaca
     9361 tggccggatt ttcccccgaa tgggttgtta tttttttgcg ggcagaaggt tactaccgct
     9421 attgaaaaac ggcgagtgcc ggtagcgata acggctgata agctgccgcg cctcgaaagg
     9481 ccgggggaca gaaccatgaa aatcgcattg atcgcggcgt tggcacttgg gcttgcggcg
     9541 gaagcccaat cgcagcccgt gaatccggcc ggagacgtga aggcgccggc cccggacgaa
     9601 tggcattcgc tgttcaacgg gcgcgatctt tcggggtgga cgatcaagat caacaaacat
     9661 ccgctggggg agaactacgc ggacacgttt cgcgtcgaag acggcgtcat caaggtgagt
     9721 tacgacggtt atggaaagtt cgacgagcag ttcggccatc tctattcaaa ccaggcctac
     9781 cagaattacg tgctgcgcct cgaataccgg atcacgggca cggccgtggc ggactcaccg
     9841 ccctgggcca agctcaacag cggcgtgatg atccattcgc aatcgccgct gaccatggga
     9901 ttgaaccagg cctggcccgc gagcatggaa gtccagtttc tcgccgtggg cgccaccgcc
     9961 ggacggcaga cgggcaacgt ctgcacgccc ggcaccaatc tcgagatgaa gggcaagctg
    10021 gtcaccgatc acatcatcga ctcgacttcg aagttgttcc cggtcgacga atgggtacag
    10081 gtggaagtcg aagtgcgcgg caacaagaca gtagtgcacc ggatcaacgg cgtggaagtg
    10141 ctgcgttacc agcatccgca gctcgatccc aaagaggaga gcgcgcaggc gctgctcgcc
    10201 gcgggagctc cgcttcagct ctccttcggt cacatcgcgc tgcaggccga atcgcaaccg
    10261 gtctggtttc gcaacatccg gattcgccag ctcgcccgat gacgttcgca ccctctacct
    10321 ggcaacgcga attgtcgcgt gccatcactg atcctgtgca attgctggcc gagctcgagc
    10381 tggatccggc cctcgctgcg ccggcgcgtg cggcatcgtc ggctttcgca ttgcgtgtca
    10441 cgcgcagcta tctcgcgcgc atgcgccgcg gagacgttga cgatccgctg cttcggcagg
    10501 tgttgccgct ggccgctgag ctcattgagt caccgggcta tggttccgat ccgctgcacg
    10561 aacacgccgc cacgcgcgcg ccgagtctcc tgcagaaata cgccggccgg gcgttgctca
    10621 tcaccaccca ggcttgcgcc atccactgcc gctactgttt ccggcgcgaa tatccgtatg
    10681 ccgaacagcg cgaggccgag gagggcgcgg gacgatggag tgcggcgctg gatgtgatcg
    10741 cgaacgacac cagcatcgaa gaagtgatcc tgagcggcgg cgatccgctg tcgatgagcg
    10801 atgcgcgcct cgcgcgcctc accgacgcca ttgccggtat gccgcatgtc cagcgcgtcc
    10861 gtgtacacac gcgccagccc atcgtgttgc cgtcacgcgt ggacgcgggc ctgttggcat
    10921 ggctgggggg tgttcgccag ccggtggtgt tcgtgctgca caccaatcac gccaatgaga
    10981 tcgacgatga agtccgcgct gcctgtgaac gactgcgccg cgggggcgcg acgctgctca
    11041 atcaatccgt gctgctcaag ggcgtcaacg acgacgtcga cacgctggcg caactgtcgc
    11101 gcgcgctcat ggacgcgggc gtggttccgt actacctgca tctgcccgat cgcgtgcgcg
    11161 gaaccgcgca tttcgatgtc gcggaggcgg acgctcagcg gctcatcgag cgcctgagta
    11221 cccgcctgtc tggctacctc gtgccgcgac tggtgcgcga ggtgcccggc gcggcgtaca
    11281 aggtacccag tgtcataacg ccctgaccac cgcctggcca gccctgatct ttgagtttcg
    11341 gcgttccaag gaaatggcag atcccgccgt ctaatgaaca gtaacgagcg gctgaacgga
    11401 tggatgaagc tctaatacgc cgagcgcagg gtggggacgc cacgtccttc gccgaactat
    11461 tggatttgca ctacgacacc atctaccggt tcgcctggaa atggtgtggc cacgcggcca
    11521 atgccgaaga tattgcccaa ctggcctgca tgaagctggc cacgagcctt gcgcaatatc
    11581 ggttccaggc ggcgttcacg agctggctct atcgactggt catcagctgc gcgcaggact
    11641 ggcaacggtc gcagcagcgt cacgaccatg acgagctgcc tgatgaggaa gcaatgaccg
    11701 aatccccgcg tcatgaagac ggcatctatc tcgcccaggt gctggcgcag ctggcgaagc
    11761 tcggcgaggg catgagggaa accgcgctgc tggtgcacgc ggaaggcatg agccatgcgg
    11821 aggccggcgc cgtgctcggc gtttcggaat ccacgatttc ctggcgcgtg cacacgattc
    11881 gcaaacacat gggccagcct gcggcccgtg cgagcctggc ctatgaatga gaaatccttc
    11941 gaatcgaaac tggcgcgcat gagcgcgccc gctcccgatc ccgaagcgcg gctccgcgcc
    12001 aagcgcgcgg cgctcgagga gttcgcgcgc gtgcaggccg cgaaccaggc aagcgcgacg
    12061 tcgacttccg catctgactc caaaggtttg tgggacgcgc tacgtctctt ccgtgacgac
    12121 tcatctcacg ggagtacttc catggcctgg tttgttagac ggaatctgtt tgccggtgtt
    12181 gccggcgcct gcgtggtcgg gctgggcatc gccatcgtgt ggcctttctt ttcgcactat
    12241 ggcgactcgc gcctgttcga cgacgtgcgc cagccggaag tcacggtcga tctgaagccg
    12301 gctgccaagg aagatcctgc tgcaccgcca gtgtcgaagt cgcctgaagc aaagcccgcg
    12361 ccagcacagc ccgagccaac acagcccgag ccggcgaaac ccgagtcccg gcgtaatcag
    12421 gccgcggtgt ccgcggcgct ggagaaggac aaggaactga atgaaatcct ggtgaccggc
    12481 gagcgcgcgc gcctgcaggc agccctggag accaagcgcg aagcggttac cgccgtcgac
    12541 agcatcgtcg cggaggatat tggcctgcca cctgacccga acgttgccga ggccttgcag
    12601 cgtattcccg gtgtccagat caccgcggga gcacagcctg cgccccaaca agtcgatgag
    12661 gagattcagg aggtaatcac gactggagtt cgcggttcgc gcagagcgca gcccgcgcac
    12721 aaaggcatgg cgccggcaac gccgggaagc tcgggtcgcg tggcggcaaa tgcgtacgcg
    12781 ccgccagcat cgccgccacc catggtgacc atgggggtga tggatgcata cgaactttcg
    12841 aagccggttg tggatgaagg gcgggatctg ttcgagaagt tcgccgttaa ccccgtcaag
    12901 caggtcgccg acgagccggt gtcgacattc tcggcggacg tggataccgc ttcgtacagc
    12961 ttcgtgcgcc gccagctgaa ctccgggcgc ctgccgcaga aggactcggt gcgcgtcgag
    13021 gagatggtca actacttcga ctatgcgtgg ccggcggcaa attccgcgaa ggctccgttc
    13081 aagccaacgg tagtcgttag cgattctccc tggggcaagg gccgcaagct ggttcacatc
    13141 ggcatcaagg gctacgaagt ctcccgcgac gacgcgccgg gcgccaacct ggtgctgctg
    13201 ctcgacgtga gcggctcgat ggattcaccg gacaaactgc cgctggcggt ccagtcgatg
    13261 gaactgctgc tgtcgagcct caagccgacc gataccgtgg gcatcgtggt gtacgcgggt
    13321 gccgcgggca cggtgctcga gccgacgccg gtgaagaaca agcagaagat catcgccgcg
    13381 ctgcgcgact tgcgccccgg tggatccacg gccggcgccg agggtatcga gcgcgcgtac
    13441 cagctcgccg aggcgcactt ccgcaaaggc ggcgtgaatc gcatcctgct ggccaccgac
    13501 ggcgacttca acgtcggcat cgccggcacg aacgagctca agggattcgt cgagcgcaag
    13561 cgcgagaagg gtattttcct ttccgtgctc ggattcggac agggcaacta ccgcgatgag
    13621 ctggcgcagg cgctggcgca gaacggcaac ggcgtggcgg cctacatcga cacgctgggc
    13681 gaagcgcaga aagtgctggt ccaggaagcc ggcgcctcgc tgttcaccat cgccaaggac
    13741 gtgaagctgc aggtggagtt caatccggcg acggtggcgg agtaccgcct cgtgggttat
    13801 gaaacccgtg cgctcaagcg cgaggacttc aacaacgatg cggtggacgc gggcgatgtg
    13861 ggctccggcc acacggtgac agccatctac gaaatcacgc ccgtgggttc ggacacgcgc
    13921 atggtagaca agagccgcta cgcgaaggag ccgcagccgc cggtcgcgac cggaaccgcc
    13981 agcgaatacg gcttcctcaa gatccgctac aaactaccgg acagcgacac ttcggagctg
    14041 atccagcagc cgatcctgat cgcacccagt gacctgcctc agaacctgtt gcgcgacgtg
    14101 cagttctcca ccgcggtggc cggcttcggg caactgctgc gcggcagccc cttcacgggc
    14161 ccgctgaagt acgacgacat catcaagcag gcgcaggctg caaagggtga cgaccccttc
    14221 ggctaccgga ccgagttcgt gcagctggtg cgcaaggcca aggtggcgcg cgagatgtaa
    14281 gacgctactt ctgcgtcttc accttgaagc cggccggtat ctcgaatgca tccgcgggca
    14341 gatcaccggt cgcgatcttc gtgaccatgg tggtgatgtc actcttggcg agtttcgaga
    14401 acatgccggc catggggttg tcgcccgtgg cgtcgacggt gacgtgagtc tccagcgcca
    14461 tgccggcctc tgccatcttg cgagtgagtt cggcgtacgc cttggcctgc gccgcgcccg
    14521 tgggtgtctt cgccgaacgc ggatcgccga aaatgaaacc ggagtccgcc gccgccttgt
    14581 agaacttctg atagtccgcg agtcccggcg cggatgtgct gaggcagacc gtgccggcga
    14641 gaaccatgtt caggtccata ccctggccag tctggccggt ggggctgaac ggcagcgaca
    14701 gcctgatgtc gtgcacggtg cacggaaagc ccgcgatctg ctttgtctgt gcggttttcg
    14761 tcagcgtcgc gttcatcgtg ccgacgccca ctttctgcag ctcatcggcg atgattgcga
    14821 gcggcgtcac cgtcgccttt ttccccttcg catcgagatc cacgaagcgg cggccgtcga
    14881 tgtcgaggat caaagactgg gccttgccgc tgaccatctg gtcactgcgc tgacgcgtgc
    14941 ccttgatctg ggtcacacca tcgccgccca ggctgatgaa agcggccttg cccgacgtgg
    15001 tggtgttgac cgtgacgtcg gcatggacgc tggcggcagc ggtggttagt agcacggaca
    15061 gcagtatctt gttagtggcc atggaccgtc agctcccttt agttagctcg gcggccgcct
    15121 gtataccgca tccggcggct acactggaat gtctcgtcct gacgacaaag tttggtgaga
    15181 atcagtccga ttccatgacc acgggacgtg cgacgttcag ccgcacgcgg ccgcgcccgg
    15241 ccttctcgag ctggacaggc gcgttctgct cggccagccg acgctgcagc aggagcaggc
    15301 gtacttcgag attgtcctgc acgtcgggca ggcgcagctc gggggcgagc cgaagttcgc
    15361 gattggaaaa ttcacgtcgg ccactgcgca ggtactcgcc caccagtttc cagaggatcg
    15421 caccggcgac tccgcggatc agatagacgt cgtcgaggaa cacgctgttg tcgcgcgaat
    15481 agcgccgcag ctgcacggcg ggaccggctt gaggtgacgc cgatgctttc ggcggctcgc
    15541 gcggtggcgg ctcgggctct ggcggctgca tgaggttcat cgccaccgcg aactgtccgc
    15601 acagcagcat gagcgcgtct tcgtcgtcgt agccgaaatg ctggtcgtgt ttgctctcca
    15661 cgagcagcgc gcccagtaca cgtccgcgcg cgcgcagcgg caccgcgagc tggctgcgtg
    15721 gttccttgag gccgggcagc ggaatctccg acttgagaac gggctcgaag ccgagcgcgt
    15781 cggcgcgatc gcgcgctgcg cgcgcgtaac gcgccatgtt catcatgtgg ccgacgcgga
    15841 tggccacgcc ttcccgcacc gccgtgccga cgatgccgtc gccggtcgag atctcggcgc
    15901 ccgcgccgcc gacctcgtaa ccacggctgg cgagcagcgt gagcacttcc tgcgcggaat
    15961 cgaacagcca caacatggcg ttgtctatgc gcagcagctc acccagcccg tctaacaagg
    16021 tgtcgagcag atccgagagc tcaccgcagt ttgccaggcg ctccgagaga atccgggaat
    16081 tggtggccag gtccacgcgc ggcgcggcgg ccggcagctc gagccggccc ggcacgcgcc
    16141 gcagttcgag cacgcggtag atgtcggcgc cgcgcaggtg aaatacctgg tccatgccga
    16201 cgtggctggc gacgcccatg agcttggcgc gcagccgctc gaacaccgga ccttcggttt
    16261 cagtgcgcag gtattcgagt tgcagcacga cgccagcgcc cgtgtagggg tcatccagcg
    16321 tcagcgcggc gcgcttcgtc gcgagaacgt tttcgcggct gcgattgaag aactggaacg
    16381 acagcgcgat gtgcagcgga tcgacgtatt cggccagcga caggtagctc acgttcggca
    16441 tgcgatccag cgacgcggtg cacagcgtgg gcggaatcac gccctccatc atgcggcgca
    16501 cgccaaacaa cgtcaggtca tccgacatcc ggcggttcat ggcttcgtca gcgcgagcgc
    16561 gctgcctgca tccggccccg gcgtctggtt gcgcgcggag aacggcgtga agcggatggc
    16621 catgagatct tcgtcagggc gcagatacca ggcctcggtg taggcgggtg gaattccgtg
    16681 gacatcgagt tgcgcgcgga atttctggta gcggtcgagc accagctcgc gaaaggcggc
    16741 gccgccatga ctgaccacgg catcgcgacc caacaggctg atcgacttgt agttctcggg
    16801 cagcgtgagg ttgagcgcga tgcggccggt atcgcgcacg gcgtcgatga cttcgaatcc
    16861 ggtctcggcg gagaggatca cgaccaggcg gccgtctggt tccagcgtgg ccgcgagccc
    16921 gcgacacagt gacggccggc catccttggt gcagccgccg acctggtggg acacacccgc
    16981 acccagcaaa tcgatgattt cctgcgtcaa cgccatgttc ttgttatcgc tcccgccgct
    17041 taacttagcg tgaatttaag aaatcgctaa gtgcgactta gcaaacgggg ggagggcatc
    17101 tccctaacct gcgccctccc ggataccgag gtcgcacatg tccaaacccg tcattaccgt
    17161 ctttggtgcc accggtgcgc agggtggcgg tctcgcccgc gcgttgctgg ccgatcccgg
    17221 ccggcatttc gcggtgcgcg cggtaacccg caagccggac agcgccgccg ccctcgagct
    17281 cgaacgcgca ggcgccgagg tggcgctggc cgacctggat gacagttgca gcgttcttcg
    17341 ggcaatggaa ggcgcctacg gcgcgttcct ggtcaccaac ttctgggaac accagtcggc
    17401 gcagaaagag ctgatccagg cgcacaacct ggcggccgcg gcggcccagg caggtgttcg
    17461 ccacgcgatc tggtccacgc tcgaggacac gcgcgattcc attgccgtgg acggcacgcg
    17521 catgcccgtg ctccagcacc agtacaacgt cccgcacttc gacgccaaag gcgccgccaa
    17581 ccggttcttc gcacaattgc gcgtcccggt gacctttctc tatacgtcgg gccactggga
    17641 aaacctgatt catttcggca tgggcccgca gcgcggtccg gacggaaagc tggcggtgac
    17701 cttcgctact gccgatgcgc gcattccgtg gatcgctgcg gaggacatcg gcatttcggc
    17761 atcggaaatc tttctgcgtg gtgacgcgct catcggggag tcgattggca ttgctggcga
    17821 tcacctgacg ggcgcggagc tggcggcgca actcggtacg gcgctggggg agcccgtgca
    17881 ctacaacgcg gtgtctcccg atgtgttccg cgggttcgcg tttcccggtg cggacgagat
    17941 cggcaacatg tggcagttca agcgcgaaac cgaaatcgcg tactgcgcac gccgcgatct
    18001 ttcgcgcgct cgggcgctgc accccgcgat ggccgatttc ggcacctggc tggcggctaa
    18061 caagtcgcgc atcccggtcg cgaaggcggc ctgagctccg atgccgcgcg cgattctcca
    18121 tgggtcgttg gcactgctgt ggctcgcagt tggctgcgcg cacggaatcg cgcgcgcgtc
    18181 cgaggcaact gcgttgcccg aacacctgcg cgacaccggc ctgtacgccg acgtttcgat
    18241 gtcgcagctg cagcccggcg tgttgccgtt ctcgccgcag tatccgctgt ggtcggacgg
    18301 cgccacgaag cgccgctgga tcaagcttcc ggccggcacc agcatcgatg ccacgcagcc
    18361 ggatgcgtgg gatttcccgc gcggcacgaa gctgtggaaa gaattcagcc acgccgggcg
    18421 ggtagaaacg cgcttcatcg aacgcgtcgt ggacgggagc tggcgttacg cgagttacgt
    18481 gtggagcgcc gatgaacagg acgccgtgct ggcaccggcg accggcatcc gcgacctgcc
    18541 ggtgccgtcg gcgcccggcg cacgatattc gattcccggg caggacgatt gtcgtgcttg
    18601 ccacgaaggt gcgccggtgc ccgtgctcgg gttcagcgca ctgcaactct cgcccgaccg
    18661 cgaccggcaa gcgccacacg cggacgctgc tgactccgcg ctttcacgca tggacctgcg
    18721 gacgctcgcc tctcgcggtc tgctggttca cctgccctca gagttgttag tgagtccgcc
    18781 gcgcatcgcg gcggcgaatc cgaccgaacg cgccgcgctg ggctatctgc acgccaattg
    18841 cggtcactgt cacaacgacg aaggcccgct tgccgtgctc gagatgtcgc tggcgcagcg
    18901 cgtcggcacg aaagagtccg cgggcagcgt gctgaggtcg atcgcctcgg tgcccggcca
    18961 gttccggatg gcaggtggca ccgcacgcct cgcgcccggc catccggatg caagtgtcat
    19021 cgcgctgcgc atgcgctcgc gcgaaccgct tcagcagatg ccaccgttgg gtacgtccga
    19081 agtcgatttc gaggctatgt cgctgattgc ccggtggatc gaaagtttgc caattcatca
    19141 acccaaatag ttagaggtgt tccgtgtatt ccctgaaaat gttcgtgaga atcgccctgg
    19201 tggtggctgc atcattcgcg cgcgctggag agcagcctgc gccggcggcc ggcagtgagg
    19261 cgttggcccg tggcaaatac ctggtgaccg tggccgggtg caacgactgt cacacgccgc
    19321 tcaagatgac gcccgaaggt ccgcggccgg acatggaccg catgctgtct ggccatccgg
    19381 aatcgctcga gatgccgccg gccccgaaga tgcccgaggg tccctggcag aatgcgtttg
    19441 cggcaacgaa taccgcgtgg tctggtccct ggggtgtgag cttcaccgcc aatctgactc
    19501 cggaccgcga gacgggtctg ggcaagtgga cggtgcgcaa tttcacggag accattcgca
    19561 cgggacgcca cctgggccgc ggacgcgaga tcctgccgcc catgcccatc ccgatgtaca
    19621 agaacttcac ggatgaggat ctggcggcga tcttcaccta cctgcagtcg attcccacgg
    19681 tgaaaaaccg ggttcccgat ccccgcccgc cggccgccct ggccagcgtc acaaagtaag
    19741 agaccggccg ccccgccccg aaaaggcggg gcggtccccc ttatagaaga gttcggttgc
    19801 aaataagaat agttctcatg tagcgttcgc actctttttg gagccgaacg ctgatggaac
    19861 cccgccgccg aatgcagctc tatcgcacga tgcatctgtg gcactggatc agcgccgcag
    19921 tctgcttcgc cgcgcttacg ctgttcaccg ttaccggcat caccctgaat cacgcgagtg
    19981 cgatcagcgc cgagcccgcc gtttcgaatg gcggcgcaca ggtgccggag gggcttcgcg
    20041 gaccgctcgc ggcgggtggc gtggaatccg cggaacctcc gtcggacatt gctgcctggg
    20101 cggaggaagc cttcaagctt tcgctgcgcg atgcgactgc ggaatggtcg gaagaggagc
    20161 tgtacctgtc ggctcccggt cccggacgcg acgcctgggt gtccatcgac cgcgccagcg
    20221 gcgccgcgaa attcgagaaa acggatcgcg gctggctggc gtacttcaac gatctgcaca
    20281 agggtcgtaa caccggcatc acctggatga tcttcatcga cgtggtcgcg gccgccgtcc
    20341 tgttcttttc actcaccggc ctcgtgttgc tgtggatcca ggcgcgtcaa cgcacgtcca
    20401 cgtggccgct ggtcggtggt ggcatcgccc tggtcaccgc actcatgatt ttcttcgcac
    20461 actgaacgga gctcagaatg attcgattga cccgcatcgc gcctggaatg ctgctcgcct
    20521 tcgccggcgg cgccagcgca gccgatctca gcctcagcgt ggaaatcccg cagctgccgg
    20581 tggctgaata tcatcgcccc tacgtggcga tctggatcga aggcgcggat caggcgatcg
    20641 ccgcaaatct cgcggtctgg taccaggtgc gtggcgatca caccaagtgg ctaccggacc
    20701 tgcggcaatg gtggcgacgc ggcggccgcg acctgaaagt tccggtggat ggcctgactg
    20761 gcgcgactcg ccccgtcggg cagcacctgt tgaaattcga cgcggcaaaa gcgccactga
    20821 ccggcctcaa gcccggccag tacacggtgg tcgtggaggc cgtacgcgaa gtcggtggac
    20881 gcgaagcggt gcgccttccg ttcgagtggc ctatcaaggc tgccaaacgt gaatcggcca
    20941 gcggcagcaa agagctcggc gccgtggcgc tgtcgctcaa tccttgagag gggaagcgca
    21001 catgaagaac tccatcgtga gtcactcagc taaattggcc gcattgctca tcgtgatggc
    21061 gccggccacc agtgaagctg cgagagcgtg gctgctaccg tcgcaaacca tcctggcgcg
    21121 ctccggcggc tgggtgaccg tggatgcggc catggccgac gacctgttct cattcaacca
    21181 gggtgcgatg caggtcgacg cgctggttgt cagcggcccc gatggacaac cggtcgcgcc
    21241 gcaaaatccg gcgaaaggca gaacccgcag cagcttcgac ctggagctgc gccaggccgg
    21301 cacgtatcgc atcgccgtcg cggaaaacat ggtgatggcg cgttgggaag aagacggcaa
    21361 gccgaaacgc tggcgtggcg cgcccgcgga aatggccacc ggcattcccg cgaacgcggc
    21421 caagctcgag gtggagcaga cacagcggcg cgtggaaact ttcgtgaccg tgggcacgcc
    21481 caccgacgtg aatcccggcg ccgggacggg catcgagctc aagcccgttc agcatccgaa
    21541 tgacctgtat gcgcgtgaaa cggcgaagtt
//
LOCUS       DASQJS010000435         8898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4055167, whole genome shotgun sequence.
ACCESSION   DASQJS010000435 DASQJS010000000
VERSION     DASQJS010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4055167"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VEW08_17160"
     CDS             complement(<1..383)
                     /locus_tag="VEW08_17160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYJ42523.1"
                     /translation="MNGFQGDFLRGALDVSPDGVVICEASGDHLVVYVNTAFCRLTGY
                     ETSDLLGKNLRLLQGEDRDQQELDTLREALRHNEAVRVLVRNQRKDGTVFVNDMQIYP
                     LRNGEGKATHLIGYHREGGTRVRTG"
     gene            complement(380..1432)
                     /gene="nagZ"
                     /locus_tag="VEW08_17165"
     CDS             complement(380..1432)
                     /gene="nagZ"
                     /locus_tag="VEW08_17165"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HYJ42524.1"
                     /translation="MTLGPLMVDVAGLELTPEDRDVLRHPLVGSVILFTRNYVNSDQL
                     ARLVDEIHAVRTPALVVAVDQEGGRVQRFRTGFSALPSPRRIGHEFDVDAKQGVAFAR
                     AMGWLMAAELRAHGVDLSFAPCVDLDLGVSEVIGDRAFHSKPEIVAQLALAYMAGMKD
                     AGMAATAKHFPGHGAVVADSHKSLPVDRREWNELGDELLPYRRLIANGIPGVMVAHIL
                     FPAVDPAPASLSRRWIQNALREEMRFEGAVFTDDLSMGGAAEFGDIVARADAALTAGC
                     DVLPVCNDRASVAKLLDELRFEAEPAAHLRLVRMRGRAAPERGELFAGGAWRVAADLL
                     AQSSAPPKLNLTAGNA"
     gene            complement(1436..2773)
                     /gene="rlmD"
                     /locus_tag="VEW08_17170"
     CDS             complement(1436..2773)
                     /gene="rlmD"
                     /locus_tag="VEW08_17170"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:NF009639.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HYJ42525.1"
                     /translation="MSKPAREGHAGIETGVVSDLTHDAEGVVRPGETSGKIAFVAGAL
                     PGERISFRRRSFHKSHDEAELIGILAASPQRVTPRCAHFGVCGGCALQHLDPAAQIEA
                     KQKELASNLERIGNVTPAEWLPPLLGPVWGYRRRARLSSRFVTRKDRSLVGFREKQGK
                     YVADVQRCEVLAEPVASLVGTLADLLTGMARRSSIPQIEVALSDGEQVLVIRVLDALP
                     EADLDKLREFERTHGLRILLQPGGLDSIVPLTPGAVDLHYRLDDFDLKLEFGPTDFVQ
                     VNAAINQAMVARAIELLEVGPSDRVLDLYCGLGNFTLPLARRARAVIGVEGEAGLIAR
                     ARANAHLNGISNAEFVVANLSVAPDTAIPWMRGGFDQVLLDPPRVGAREVLSAVAHLA
                     PRRVVYISCHTGSLARDLGVLTHELGFRLRAAGVLDMFPHTTHVESIAVLDRN"
     gene            complement(2770..3546)
                     /locus_tag="VEW08_17175"
     CDS             complement(2770..3546)
                     /locus_tag="VEW08_17175"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYJ42526.1"
                     /translation="MNTLVFDIETVPDVEFGRRLFGLEGLSDDEVGKAMQAKARQDSG
                     SDFLPHEQHRIVAISCAFRSREGFRVWSLGDEGASERELVERFFDGIDKFTPDLVSWN
                     GGGFDLPVLHYRALRFKVQAPRYWETGDEDNAFRYNNYLGRFHWRHMDVMDVLSGYQN
                     RARASLSDTAVLLGFPGKLGFDGSQVWDAYRSGELTRIRRYCETDVINTWLIFLRFQH
                     MRGRLTDAGLADELARTRTWLTDAKQAHFDEFLAAWTPET"
     gene            complement(3543..4016)
                     /gene="acpS"
                     /locus_tag="VEW08_17180"
     CDS             complement(3543..4016)
                     /gene="acpS"
                     /locus_tag="VEW08_17180"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HYJ42527.1"
                     /translation="MIFGIGVDVLEAARIKKVLARFGDHFIDRLLMPAERVQLGRTKR
                     PERFIAMRFAAKEAIVKAMGTGFAHGIWIRDVGVVQNAWGKPEVAYSPRGEKVRRQLG
                     IGDGHVTLTDEAGLIVAVAVLEAAPKSRPAPKRTAKRLPKPKPKRKAKRLAKVRR"
     gene            complement(4013..4747)
                     /gene="pdxJ"
                     /locus_tag="VEW08_17185"
     CDS             complement(4013..4747)
                     /gene="pdxJ"
                     /locus_tag="VEW08_17185"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014605257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HYJ42528.1"
                     /translation="MRNSTIELGINIDHVATVRQARRVSYPDPVHAALLAEQAGADNI
                     TLHLREDRRHIQDRDVHALRPLLQTRMNLEMGLTDEMVQIAVGVRPQDCCLVPEKREE
                     ITTEGGLDVAADVERVAGGVRKLAAAGIRVALFIAPDLAQIEAAKRSGAPVIELHTGT
                     YAEATGSAQARELERLSTAAKRAANLGLEVHAGHGLTYNNVLPVAAIPEIVELNIGHC
                     IIARAIFTGLDAAVREMKTLMQSARR"
     gene            complement(4744..5445)
                     /gene="recO"
                     /locus_tag="VEW08_17190"
     CDS             complement(4744..5445)
                     /gene="recO"
                     /locus_tag="VEW08_17190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HYJ42529.1"
                     /translation="MQPRERIALEPAFVLHHREYRDTSRILDVFTAKHGRLTLFARGA
                     RGPKSKLASLLMPFRPLLVTWTGRGDAAQLTAAEPDGDALAFPPRHVLSGFYLNELII
                     HLTTRHDPQPQLFSDYDHALRRLATDAAPELALRVFEKRLLESIGYGLEFVVDTAGYY
                     QFRPTQGLSQVREDAPGAYSGRCLLALQTETLGDTETLDVARRVLRQVLDHCLEGREL
                     RTRTVARSMSRRGNA"
     gene            complement(5456..6376)
                     /gene="era"
                     /locus_tag="VEW08_17195"
     CDS             complement(5456..6376)
                     /gene="era"
                     /locus_tag="VEW08_17195"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HYJ42530.1"
                     /translation="MTEENIPGTGPATPFRAGFAALVGRPNVGKSTLLNALVGEKLSI
                     VTSRPQTTRHRVLGMLNRPGVQIAFVDTPGLHLGEKRALNRAMNRTAASALADADLVV
                     FVVEGMRFYEEDEAVLQRIRESGRNSIAVINKVDLIYPKDQLLPFTAELAGKHGFLDV
                     VPVSAEKGVNLDRLIDVITARLPESPELFPPEQRTDRGEDFQISESIREKLTMELVEE
                     LPYGIAVEIEKKEEVDGRLVIDAVIWVDRAGQKPIVIGAKGSRLKRIGQQAREELNAR
                     LGKRLHLNLWVKVREDWADDARALAQLGME"
     gene            complement(6369..7052)
                     /gene="rnc"
                     /locus_tag="VEW08_17200"
     CDS             complement(6369..7052)
                     /gene="rnc"
                     /locus_tag="VEW08_17200"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HYJ42531.1"
                     /translation="MPSSQADTLAARLGYAPRDRALFVVALTHRSAEGPNNERLEFLG
                     DSVLNLLLSERLYREFPLASEGDLSRLRARLVSEEPLAEIAQEMQLGELLLLGSGELK
                     TGGFRRQSILADAFEALCGALYLDGGPDVVRTIIGPMFESRIAGLPEPSTLKDAKTRL
                     QEHLQGNARSLPVYTVKRTSGEPHEQTFLVSCAISQPSLETEGEGPSRRRAEQAAAQA
                     ALRALGLYD"
     gene            complement(7076..7438)
                     /locus_tag="VEW08_17205"
     CDS             complement(7076..7438)
                     /locus_tag="VEW08_17205"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HYJ42532.1"
                     /translation="MRKQRGVTFIGWIFLLAPMAVVLYAGIRVGPEYLNYYKVVTAMK
                     TTATQLKTEEALSPDGIRSSLGRRFDTGYIDSPSVKEIVVTKADAGWTMTADYEKTLP
                     MFGNLYITLVFNKSVVIN"
     gene            complement(7456..8601)
                     /gene="lepB"
                     /locus_tag="VEW08_17210"
     CDS             complement(7456..8601)
                     /gene="lepB"
                     /locus_tag="VEW08_17210"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HYJ42533.1"
                     /translation="MHEIIDLLKQVAVPASWLAIPVGLWCAIDSWLLAPRRATDAGSL
                     VVPDPPLVRIAYAVLPWLVVAVILRLITAETLDFSLVLLLLSIGTGIVWGIDQLFFRK
                     PRDAAAAAAQPAPIALPEPGTVDYARSFFPVAFIVLIVRAFIFEPFRIPSDSMMPTLL
                     DGDFIVVNKFSYGLRWPVVNEKFIDIGSPQRGDVVVFRHPPHPNVNYIKRLVGLPGDR
                     IEVHDDHLVINGQKIALQEVGDYTDGCYVGLKLMTETIGEHTHQVMSCRSDVGLIPGN
                     RFLDEGSSANLPACDRKKTRADVGRWVCRETPANTGRDRGDHQFEIVPDGYYLMIGDN
                     RDNSDDSRGWGLVPDQNLVGKATRIWFNFDLQRGSVVNWSRIGDSIP"
     gene            complement(8605..>8898)
                     /locus_tag="VEW08_17215"
     CDS             complement(8605..>8898)
                     /locus_tag="VEW08_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HYJ42534.1"
                     /translation="HKDSAYERGRQLVDKMQELIPRQMFEVAVQAAIGSHVIARATVK
                     ALRKNVLAKCYGGDISRKKKLLEKQKEGKKRMKNIGSVEIPQEAFLAVLKVGK"
BASE COUNT         1565 a         2855 c         2891 g         1587 t
ORIGIN      
        1 tccccggtgc gcacgcgggt tccgccttcc cggtgatagc cgatgagatg cgtcgccttc
       61 ccctcgccgt tacgcagcgg gtagatctgc atgtcgttga cgaacaccgt gccgtccttg
      121 cgctggttgc gcaccagaac ccgcacggcc tcgttgtggc gcagcgcttc gcgaagcgtg
      181 tcgagctcct gctgatcgcg atcctcgccc tgcagcagcc gcaggttctt gcctaacagg
      241 tctgacgtct catagccggt gagccggcag aacgccgtgt tgacgtacac caccaggtga
      301 tcaccggaag cctcgcagat gaccacgcca tcgggcgaga cgtcgagagc gccacgcaga
      361 aaatccccct ggaaaccgtt catgcgttgc ccgccgtcag atttagcttg ggcggtgccg
      421 aggactgtgc cagcagatcc gctgcgacac gccacgcgcc gcccgcaaag agttcgccac
      481 gttcgggcgc ggcccggccg cgcatgcgaa ccagccgcag gtgagccgcg ggttcggctt
      541 cgaacctgag ttcatcgagt agtttggcaa ccgaggcgcg gtcgttgcac accggcagca
      601 cgtcgcaacc ggcggtgagt gccgcatcgg cgcgcgccac gatgtcgccg aactctgcgg
      661 cgccacccat cgaaagatcg tcggtgaaca ccgcgccctc gaaccgcatt tcttcgcgca
      721 acgcgttctg gatccagcgg cgcgagagac tggccggcgc cgggtcgacg gcggggaaca
      781 ggatgtgggc caccatgaca ccggggatgc cgttggcgat gagccggcgg tatggcagca
      841 gttcgtcgcc gagctcgttc cattcgcgtc tatctacggg cagcgacttg tgcgagtcgg
      901 cgacgaccgc accgtggccg ggaaaatgtt tggcggtggc ggccatgccg gcgtctttca
      961 tgcccgccat gtaagcgagc gcgagctgcg ccacgatctc gggtttcgaa tggaacgcgc
     1021 gatcaccgat gacctcgctc acgccgagat ccagatcgac gcacggggca aaagacaggt
     1081 cgacgccgtg cgcgcgcagc tcggccgcca ttagccagcc catggcgcgg gcgaacgcca
     1141 cgccctgctt cgcgtccacg tcgaactcgt ggccgatgcg gcgcggcgac ggcagcgccg
     1201 agaaacctgt gcggaatcgc tggacacggc caccctcctg gtcgacggcc accaccagtg
     1261 ccggggtgcg cacggcatgg atttcgtcca ccaggcgagc gagctgatcg gaattgacgt
     1321 agttgcgcgt gaacaggatg acgctgccca cgagcggatg gcgcagaaca tcgcggtcct
     1381 cgggcgtcag ctcgagaccg gccacgtcga ccatcagtgg gcccaaagtc acaactcaat
     1441 tcctgtcgag tacggctatc gattccacgt gtgtggtgtg cgggaacata tcaagtacac
     1501 cggcggcgcg taagcggaag cccaactcat gcgtcaggac gcccaagtcg cgtgccaggg
     1561 agcccgtgtg gcaggagata tacaccaccc tgcgcggggc gagatgtgcc acggcactca
     1621 aaacctcacg cgcaccgact cgcggagggt ctaacagcac ctggtcgaac ccccctcgca
     1681 tccatggaat agcggtgtcg ggcgccaccg acaggttagc cacgacgaat tccgcattgg
     1741 aaatgccatt caggtgagca ttggcacgcg cccgggcaat gagacctgcc tctccttcca
     1801 cgccaattac cgcccgggcc cggcgcgcca ggggcagcgt gaagttgccg aggccgcagt
     1861 acaggtccag gacccggtcg gatggcccga cttccagtag ttcgatggcc cgggcgacca
     1921 tggcctggtt gatggcggcg ttcacctgga cgaagtcggt ggggccgaat tccagcttca
     1981 ggtcgaaatc gtcgagacga tagtgcaggt cgaccgcgcc tggcgtcagg ggcacgatgg
     2041 agtcgagccc gcctggctgc agcaggatgc gcaggccgtg cgtccgctcg aactcgcgca
     2101 gcttgtcgag atcggcctcg ggcagcgcat ccagcacgcg aatgaccagc acctgttcgc
     2161 catcggacag cgccacctcg atctgcggga tgctcgaccg gcgcgccatg ccggtcagca
     2221 ggtccgccag cgtgccgacc agcgacgcga ctggctcggc cagcacctca caacgttgca
     2281 cgtcagcgac gtacttgccc tgcttctcgc gaaaaccgac gagactccgg tctttcctgg
     2341 tgacgaagcg cgacgacaac cgcgcgcgcc ggcgatagcc ccaaacggga cctagcaacg
     2401 gtggcagcca ctcggcaggc gtgacgttgc cgatgcgctc gaggttcgat gcgagctctt
     2461 tctgtttggc ctcgatttgc gcggcgggat ccaggtgctg caacgcgcag ccgccgcaca
     2521 caccgaagtg cgcacagcgc ggtgtcacac gctgcggcga agccgcgagt atcccgatga
     2581 gctcggcttc gtcgtggctc ttgtggaagc tgcggcgccg gaaactgatg cgctcacccg
     2641 gcagcgcacc ggcgacgaag gcgatcttgc cggaggtctc gccgggccgc accacgcctt
     2701 cggcgtcgtg cgtgagatcg ctgaccacac cggtttcgat gccggcatgg ccttcacgtg
     2761 caggcttgct cacgtttcgg gcgtccaggc ggcgaggaac tcgtcgaagt gggcttgttt
     2821 ggcatcggtg agccaggtgc gcgtgcgcgc cagctcgtcg gcgaggccgg cgtcggtgag
     2881 ccggccgcgc atgtgctgaa agcgcaggaa tatgagccag gtattgatga cgtccgtctc
     2941 gcagtaacgg cggatgcgcg tgagctcgcc actgcgatag gcgtcccata cctgcgatcc
     3001 gtcgaaaccg agcttgccgg gaaaacccag caacaccgcc gtatcggaaa gcgaggcgcg
     3061 ggcgcgattc tggtagcccg aaagcacgtc catgacgtcc atgtggcgcc agtgaaagcg
     3121 gccgagatag ttgttgtagc gaaacgcgtt gtcttcgtcg ccggtttccc agtaacgcgg
     3181 cgcctgcact ttgaaacgca gcgcgcgata atgcagtacg ggaagatcga agcctccgcc
     3241 gttccagctc accaggtcgg gcgtgaattt gtcgatgccg tcgaaaaagc gctccaccag
     3301 ctcacgttct gacgcgccct cgtcccccag gctccacacg cgaaatccct cgcgcgaacg
     3361 gaaagcacag gaaatggcga cgatgcgatg ttgctcgtgg ggcaggaagt cgctgccaga
     3421 atcctggcgc gccttcgcct gcatggcctt gccgacttca tcatcggaaa gcccttcgag
     3481 cccgaagagg cgccgcccga actccacgtc cggcacggtt tcaatgtcga aaaccagcgt
     3541 gttcatcggc gaaccttcgc caggcgtttc gccttacgct ttggcttcgg cttcggcagg
     3601 cgcttcgcag tgcgtttcgg cgcgggtctc gatttgggcg ccgcttcgag cacggccacc
     3661 gcgacgatga gccccgcttc gtcggtaagc gtgacgtggc cgtcaccgat tcccaactgg
     3721 cggcgcacct tttcgccgcg cggtgaatat gcgacttcgg gtttgcccca cgcgttctgt
     3781 accacgccga cgtcacggat ccagatgccg tgcgcaaagc ccgtgcccat ggccttgacg
     3841 atggcttcct tggcggcgaa acgcatggcg atgaaccgct cgggccgctt cgtccggcca
     3901 agctgcacgc gttccgcggg catgagcagc cgatcgatga aatggtcgcc gaatcttgcc
     3961 aggaccttct tgatgcgcgc ggcctcgagc acgtcgacac cgatgccgaa gatcatcggc
     4021 gcgcactctg catcagcgtt ttcatctcgc gcaccgcggc atcgagaccg gtgaaaatcg
     4081 cgcgcgcgat gatgcaatgg ccgatgttga gctcgacgat ttcggggatg gccgccaccg
     4141 gaagcacgtt gttgtaggtg agcccatggc cggcgtgcac ttccagcccc aggttggccg
     4201 cgcgtttggc ggcggtcgac aggcgttcga gctcgcgcgc ctgagccgac ccggtggctt
     4261 cggcgtatgt gccggtatgc agctcgatga ccggcgcgcc cgagcgcttg gccgcttcga
     4321 tctgcgcgag atccggcgcg atgaacaatg ccacgcgaat gcccgccgcc gcgagcttgc
     4381 gcacaccgcc ggccacgcgt tccacgtcgg cggcgacatc cagcccgcct tcggtggtga
     4441 tttcttcgcg cttctccggc accaggcagc agtcctgtgg ccgcacgccc acggcgatct
     4501 gcaccatctc atcggtgagt cccatctcga ggttcatgcg tgtctgcagc agcggccgca
     4561 aggcgtgcac gtcgcgatcc tggatgtgcc ggcggtcctc gcgcaggtgc agcgtgatgt
     4621 tgtcggcgcc ggcctgctcg gccagcagcg ccgcatgcac cggatccggg tacgacacgc
     4681 ggcgcgcctg gcgtaccgtg gccacgtgat cgatgttgat gccgagttcg atggtcgaat
     4741 tcctcatgcg tttcctcgtc gcgacataga gcgcgccaca gtgcgcgtgc gaagctcgcg
     4801 gccctccagg caatgatcca gcacctggcg caacacgcgg cgcgccacgt cgagcgtctc
     4861 ggtgtcaccc agggtttcgg tttgcagcgc cagcaggcag cggccggaat aggcgcccgg
     4921 ggcgtcttcg cgaacctgcg agagtccctg cgtcggccgg aactggtagt agcccgcagt
     4981 gtcgacgacg aattcgaggc catatccgat ggactcgagc aaccgcttct cgaaaacgcg
     5041 caacgccagt tcgggcgcgg cgtccgtcgc gagccgccgc agtgcgtggt cgtagtcgct
     5101 gaacaattgc ggctgtggat cgtgacgcgt cgtcagatgg atgatgagct cgttgaggta
     5161 gaaaccggag agcacatggc gtggcggaaa tgccagggcg tcgccgtccg gctccgcggc
     5221 cgtcaattgc gcggcatccc cacggccggt ccatgtcacg agcagcggcc ggaatggcat
     5281 gagtaatgag gcgagtttcg acttgggtcc gcgcgcgccg cgcgcgaaca gcgtcagccg
     5341 tccgtgcttc gcggtgaaca catcgagaat gcggctggtg tcgcgatact cgcgatgatg
     5401 cagcacgaag gccggctcga gagcgatgcg ttcgcgcggc tgcatggtca cctcactact
     5461 ccatcccaag ctgcgccagg gcgcgcgcat cgtccgccca gtcctcgcgc actttgaccc
     5521 agagattgag gtgcaggcgt ttgccgagcc tggcattgag ctcttcgcgc gcctgctggc
     5581 cgatgcgctt caggcgtgaa cccttggcgc cgatgacgat gggcttctgc ccggcgcgat
     5641 ccacccagat gaccgcatcg atgacgaggc gaccatctac ttcttccttc ttttcgattt
     5701 cgaccgcgat gccgtaggga agctcttcca cgagttccat cgtgagcttc tcgcggatgg
     5761 attcggagat ctggaagtcc tcgccgcggt cggtgcgttg ctcgggcggg aacagctccg
     5821 gcgactcggg cagacgcgcg gtgatgacgt cgatgaggcg gtcgagattc acgcccttct
     5881 cggccgacac cggaaccacg tcgagaaacc cgtgtttgcc cgcgagctcc gccgtgaatg
     5941 gcagcagctg atccttggga tagatgaggt cgaccttgtt gatgacggcg atcgagttgc
     6001 gcccggattc gcggatgcgc tgcaggacag cttcgtcttc ctcgtagaag cgcatgccct
     6061 cgacgacgaa cacgaccagg tccgcatcgg cgagcgccga ggcggccgtg cggttcatgg
     6121 cgcggttcaa cgcgcgcttt tcgccgagat gcagacccgg cgtatcgacg aacgcgatct
     6181 gcacaccggg gcgattgagc atgccgagca cgcggtgacg cgtggtctgc gggcgcgagg
     6241 tgacgatgga aagtttttcg ccgacgagag cattgagcag cgtcgacttg ccgacgtttg
     6301 gacgtccgac gagagcggcg aaccccgcac gaaaaggggt ggccggcccc gtgccgggga
     6361 tgttttcttc agtcatagag tcccagcgcg cgaagcgcgg cctgtgcggc tgcctgttcg
     6421 gcgcggcgcc ggctcgggcc ttcgccctcg gtttcgagcg agggctgcga gatggcgcag
     6481 gaaaccagga aagtctgctc atgcggttcg ccgctggtgc gcttgacggt gtagacgggc
     6541 agcgaacggg cgttgccttg caggtgctcc tgcaggcgcg tcttcgcatc cttgagcgtg
     6601 gacggctcgg gcaggccggc gatgcggctt tcgaacatcg ggccgatgat cgtacgaacg
     6661 acatcgggtc caccgtcgag atacaacgcg ccgcacagcg cctcgaaagc atcggcgaga
     6721 atggactgcc ggcggaagcc gccagtcttg agttcgccag atcccagcag caataactcg
     6781 ccgagctgca tctcctgggc gatttcggcc agtggctctt cactcacgag ccgcgcgcgc
     6841 aggcgcgaca ggtcgccttc gctggccagc ggaaactcgc gatacagccg ctcggacaac
     6901 aacaggttca agaccgagtc accgaggaat tcgagccgct cgttgttagg accttcggcg
     6961 cttcgatgcg tgagcgcgac gacgaacagt gcgcggtcgc gcggcgcata gcccagcctg
     7021 gcggcaagcg tgtccgcctg actggacggc atcagcccca tgagtgcagc agcattcagt
     7081 tgatcactac cgatttgttg aagaccagcg tgatgtagag attgccgaac atgggcaagg
     7141 tcttttcata gtccgcggtc atggtccagc ccgcgtctgc cttggtgacg acgatttctt
     7201 tgacagaggg cgagtcgatg tagcccgtgt cgaaccgcct cccgagcgat gagcgaatgc
     7261 cgtccggact cagcgcttct tcggtcttta gctgcgtggc ggttgttttc atcgccgtca
     7321 ccactttgta gtagttcagg tattccggtc caacgcggat gccggcgtag agcaccacgg
     7381 ccatcggtgc cagcaggaag atccagccga taaaggtcac accacgttgt ttgcgcatat
     7441 accctcccgg gacgttcacg gaatgctatc gccgatgcga ctccagttga ccacgcttcc
     7501 acgctggaga tcaaaattga accagattcg agtcgccttg ccgaccagat tctggtcggg
     7561 cacgagaccc cagccgcggc tgtcatcgct gttgtcgcgg ttgtcgccga tcataaggta
     7621 gtagccgtcc gggacgatct cgaactggtg gtcgccgcgg tcgcgcccgg tgttggcggg
     7681 cgtctcccgg cagacccagc gacccacgtc tgcgcgggtc ttcttgcgat cgcaggccgg
     7741 caggtttgcc gagctgccct cgtccaggaa acggttgcca gggatgagcc cgacatcaga
     7801 ccggcacgac atgacctggt gggtgtgctc gccgatcgtt tcggtcatga gcttgaggcc
     7861 cacataacag ccgtcggtgt agtcgccgac ttcctgcagc gcgatcttct ggccattgat
     7921 cacgagatga tcgtcatgca cttcgatgcg atcgccgggt aacccgacca ggcgtttgat
     7981 gtagttcacg ttggggtgcg gcgggtgacg gaacaccacc acgtcgccgc gctgcggcga
     8041 tccgatgtcg atgaacttct cgttcaccac cggccaacgc agaccgtatg agaacttgtt
     8101 cactacgatg aaatcgccgt cgagcagcgt gggcatcatc gagtccgagg gaatgcggaa
     8161 cggctcgaag atgaacgcgc gcacgatgag cacgatgaac gccaccggaa agaaactgcg
     8221 ggcgtagtcg acggtgcccg gttccggaag cgcgatgggc gctggctgcg ccgcggccgc
     8281 ggccgcgtcg cgcggctttc ggaagaacag ctggtcgatg ccccagacga ttcccgtgcc
     8341 gatcgacagc agcaggagca ccaggctgaa gtcgagcgtc tccgcggtga tgagtcgcag
     8401 gatgacggcg accaccagcc acggaaggac ggcgtaggcg atacgcacca gaggcggatc
     8461 cggcaccacc agtgaacctg cgtcggtcgc acgtcgtggc gccagcaacc agctgtcgat
     8521 tgcgcaccac aggcccaccg ggatcgcgag ccagctcgcg ggcacggcga cttgcttcaa
     8581 caggtcgatg atctcgtgca tgggctattt ccccaccttg agcacggcaa ggaacgcttc
     8641 ctgcgggatc tccaccgagc cgatgttctt catgcgcttc ttgccctctt tctgcttctc
     8701 gagcagtttc ttctttctcg agatgtcgcc gccgtaacac ttggcgagca cgttcttgcg
     8761 cagcgccttg acggtggcgc gcgcgatcac atggctgccg atggcggcct gcaccgcgac
     8821 ttcgaacatc tggcgcggaa tcagctcctg catcttgtcg acgagctgac gaccacgctc
     8881 gtaggcgctg tccttgtg
//
LOCUS       DASQJS010000436        11501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3737220, whole genome shotgun sequence.
ACCESSION   DASQJS010000436 DASQJS010000000
VERSION     DASQJS010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11501
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3737220"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VEW08_17220"
     CDS             <1..1069
                     /locus_tag="VEW08_17220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative urea ABC transporter substrate-binding
                     protein"
                     /protein_id="HYJ42535.1"
                     /translation="RRLLQTFFIAVLAGAALLTTQAARAAEKTTFNVAWSIYVGWMPW
                     DYAQSSGILKKWADKYGITIKLTQMDYIESINQYTAGKFDGCVMTNMDMLTIPSAGGV
                     DSTALIVGDYSNGNDGVVLKGAGKKLADIKGQSVNLVELSVSHYLLARGLESVGLAER
                     DIKVVNTSDADIVSAFATPGVTATVTWKPQLSEVTAMPKTTLVFDSSKTPGEILDLMV
                     VNSAVLKANPKLGKALVGAWYETLALMLAKDAKATAALESMAKASGTDLAGFKGQLAT
                     TFLYSTPASAVAATKAPDLVKKMDLVRKFSFDHGLLGEGAKTVDAVGIEFPGGVTLGN
                     KANLKLRFDLGYMQMALDGKL"
     gene            1080..1895
                     /locus_tag="VEW08_17225"
     CDS             1080..1895
                     /locus_tag="VEW08_17225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ42536.1"
                     /translation="MRRLINQHPGRAAGLALGVLPFIALLVMYVIASHLRLAENPDDK
                     LLPALGKIASTFHSYAFEEDSRTGRVLFWADTWASLKRIGSALLISATLGMILAVGIG
                     VIPRVRALLSPLLGTIAMIPPLAILPVLFIVLGLEETAKISLIVIGIAPCIARDLALR
                     VGELPKEQIIKAQTLGASTWQLILRVVLPQLWPRLIDSLRLSMGSAWLFLIAAEAIAS
                     TEGLGYRIFLVRRYLAMDVILPYVVWITLLAVLTDLALRLIRTHAFRWAEPER"
     gene            1895..2641
                     /locus_tag="VEW08_17230"
     CDS             1895..2641
                     /locus_tag="VEW08_17230"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ42537.1"
                     /translation="MAAVTVRNVWKEYGAQVVLENVRLEIADHEFVTIVGASGCGKTT
                     FLKMLLGMEQPTRGQILIDGVPIRPEPDPDRGVVFQRYSAFPHLTVIDNVMLGLELRS
                     SKITGRLFGAARREARAAAMAMLESVGLAHAADRYPAQLSGGMQQRMSIAQAVIRKPK
                     VLLLDEPFGALDPGVTADMHELILRLWEENRMTIFMVSHDIQESFQLGTRLLTFDKLR
                     HDPQSPEAYGASVTYDMPLSRRRPRAAPSS"
     gene            complement(2651..3664)
                     /locus_tag="VEW08_17235"
     CDS             complement(2651..3664)
                     /locus_tag="VEW08_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42538.1"
                     /translation="MYRKFPLLVVALTLPSAASAQIVFPPSALLPTPVLSTPAAGATV
                     KGEVPALTTVRFAWVEYGLFSTPSQPLPTHFLVCVKLASQAANCTFGPADFLPVGALP
                     HVTVYDGSLAIGYRYTYDMPAGSIADSLLNVDTNWAVGACRNAVAANCVFSAPRPLWM
                     STLDLNAQNIADDSSSTHAIFRAQTRNGGSTNLASIPPVETSIFAWDAVKDPVTSQCR
                     TDIDSPDVRNDPDIIVIMDNGATRWIQTLPIINGVRTAPVLVVGMHRWNKGLTVRGEE
                     DFLQYDLPPGGISQNATTLSFELPKAQRPKPFVVTMTADRNGAVKEFDENNNSGAACE
                     SVP"
     gene            complement(3683..8713)
                     /locus_tag="VEW08_17240"
     CDS             complement(3683..8713)
                     /locus_tag="VEW08_17240"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HYJ42539.1"
                     /translation="MSRKPTMPIFDRSRAFFASLFALTLSACSGSPGLPPDDDDPTTP
                     PAAATWSKSIGGPGDDWANAVVATRDGGFLLAGVFDHQQTDESGLPPIDGDFWVTKLD
                     QAGDLDWQLALGEKRAAAAGAEALFKRVRAGDDGYWMVGTQVGPGPSGDVSNPDAVVD
                     LLVARLNDDGSVRWTRSYDGGGFASREFFRANESARDTGWDVWPTPDGGALLAAWSSA
                     VVRVDASNGIPVAAPWIVKVNATGIVQWSRRISETQYDYLPEEPAELLVRATAQNGAV
                     VAASARVRGDSTEQGPKPAMQLSLLDDGGGVPWTRRYSRLIARDLIQVEDANDDDVFD
                     GGAEDGFVIVGDDIAIWYTLDGDRDSIVMKFSREDGDEEWRSVIEEGALFETVVQMCR
                     PTPSLARYCHYVAGGRGSRDDEGALLAMSAYVEPDGDLFTTEFYDGLEILSDLRIAAP
                     GAADANTPLQAVGYTSDGGTFRMLIRPNLTEVSRDSAAYGPADGETYPLPALQFDRDA
                     RVVSVYQPGDRAHIQVFGNAGEPLVDRNFGAGSEFRGERAFATVELSNGFVVAGQSDS
                     TGDVELPGTVVAKLRLDTNVGDVLWQVRLDNFSLLSRKHLPYGAIAASTDGGVVLAGV
                     SATEGVAPQPRVIKLDATGAVLWSTAPLNPNPSPQPIATGIVARSDGGYYVIGEDLEF
                     IQSAAPWVTRIDATGGIVWQRQLPIGGALSGIDVTSDGGVIVAGVAGGQAMQLPWAAR
                     ISAAGALEWSYSFEVGGSLYGPLARIVTLADGGFLLASSHLDGGAFNDESALAAAGRR
                     NILLIRLAADGAARWHRTYGGLYDETLFGLDALADGGFVVAGRSDSLGERGEAWVMRL
                     GPDGRINEGCNALRTAELATNYAPLTLGMRDRSTAAGRRIAGNAASRASAVPTQLIPV
                     PAVARQCAGFTQPDAGGTPPLRHRLNVVQGNVSSPGVVTSAPGGISCGTGANRCEAAY
                     LPNTTVTLAVDSGERGRFLGFTGCDIATAYSCILTMTADRTVTATFRAANAPRFTLNV
                     TGNGRVSGAGLDCSNGSSGTCGGTYSPGEILTLSATASATETFVGWGGACAAFARGSP
                     INLEMTGNLTCSAEFTGGPPGSPRVTVTLSPAGAGPQVGYVRSVPVGITCGSMGADCT
                     QTFATGSTVRLEGISTDANYSFETFTCTGLADVPGARSVEFAITTDVSCQGRFATNIE
                     RLQMRLVSDTLTGIGIPGRVIAVPPTAGGSMDCTADCDRPFARHSIVLLHAEPGINRY
                     LSGWSGCDAETLDPQGTFGQTVCHVGMQRTRNVVAFFSGIGIGSDGIRQLAISFPYDS
                     GAGTVTANRPTDIPPCVPGGNACQIIYDIADEVLLTIVPNGNNTLTLNEGCDPFTPAT
                     ATAAATCRVLMDRDRGVVFNFGTVNAVPVAVITSDAAGSVGVGQAVGFSASSSTDDGG
                     NTQLTYQWDFDENGSVDSTAVEATYAWSAPGLYRVRLAVTDSTGARDTDEVEVTVVAS
                     NNPPVASFTYSPGSPQTGNAVSFDATSSTDDVGIGIYRWDFDGDGVTDAEGIYANARV
                     RQFTYAAAGTYLARLTVTDAGGLSDQTTRTLTVTSAPGALDLTLMLRGGGHGVVTYVP
                     VVTACSKDSEPTCVRQFAADTVVVLTASAYSGSLLGDWVGCTSLNADGTQCTVNLTSS
                     RTVTLFIN"
     gene            complement(8808..>11501)
                     /locus_tag="VEW08_17245"
     CDS             complement(8808..>11501)
                     /locus_tag="VEW08_17245"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYJ42540.1"
                     /translation="GERIGPYRLVRPIGTGGMGTVWLAQRADGLFERGVALKLPHRDL
                     FGARLVERMARERRILASLDHPNIARLYDAGMTDDGQPYIALEFVDGVRVDHYCAEKR
                     CDTRQRLQLFAQVAHAVAYAHGKLVVHRDLKPANILVTHDGQVRLLDFGIAKLLDDEA
                     GSDAQLTQVSARVFTPDYASPEQIAGLPITVATDVYSLGVVLYELLTGSRPYRLKRES
                     RAALEEAILEAEARRPSDAAAHAVARRAMRGDLDAIVLKALHKRPEGRYATVHAFADD
                     IARHLAGRPVSAQPDRALYRIGRFLKRHKLATAVTAMVVAALLGATGTALRQMQLARD
                     EQHRAEDIKRFIATIFLNADPQEGGGRSVTAQALLQQARSRIDRDFVNRPEIRVELLT
                     TVAASLANLDDYDSAEQITNEALAISRVAFGDRHPLTREARMVRLDIERTRGRTAQLR
                     KEVDELLAAARASKDTTPTELIRLLVFDAHAAIDEGRPELAIRAMNEALPLALQTLGE
                     RNQLTVGAAVVRAIAFRSAGRPLEAVDAAREALRLSLTLVDGNQKHAMVVDARMTYGG
                     TLIQAGQPAEAVNELQRVLVDAVAVLGEDSMLSGFASSFLARAQLDTGDMQGALRNSE
                     RSLTLIRKWNEPRSYTTGAALATRGRTLLLARRPHEAHEAFEQSLDILKATIGPDHDT
                     TAVVDANFQLARAALGEIAAAETALRKLQASGRKLDHWMSVSAPHALGIVQRLAAQPV
                     AAMAQQRAALAALAALADDPRAKFRRMAIVTDLGLAALDAGDYASATAALTEALATTQ
                     REQTQLSPIRADALLGMARVRLHEKEFANAMIYAQEARDYWRTLNGPSRELALANLWL
                     ARCEFAAGQRQAASKRTAEARALLAKSPLPVDQKVLRDTAN"
BASE COUNT         1919 a         3701 c         3854 g         2027 t
ORIGIN      
        1 acgccgtctg ctgcagacct tcttcattgc tgtgctcgct ggcgcagccc tgctgaccac
       61 ccaggccgcg cgcgcggccg aaaaaaccac gttcaacgtc gcctggagca tctacgtcgg
      121 ctggatgccc tgggactacg cgcaatcgag cggcatcctc aagaagtggg ccgacaagta
      181 cggcatcacc atcaagctca cgcagatgga ctacatcgag tccatcaacc agtacacggc
      241 cggcaagttc gacggctgcg tcatgaccaa catggacatg ctcacgatcc cctccgcggg
      301 cggcgtggat tccaccgcgc tcatcgtcgg cgactattcc aacggcaacg acggcgtggt
      361 gctcaaggga gccggcaaga agctcgccga catcaagggc cagtcggtga acctcgtcga
      421 gctctcggta tcgcattacc ttctggcgcg cggcctcgaa tcggtcgggc tcgccgaacg
      481 cgacatcaag gtcgtgaaca cttcggacgc ggacatcgtc agcgccttcg caaccccggg
      541 cgtgaccgcc acggtgacct ggaagccgca gttgtccgaa gtcacggcaa tgccgaagac
      601 cacgctggtg ttcgattcga gcaagacgcc gggcgaaatt ctcgatctca tggtggtcaa
      661 cagcgcagtg ctgaaggcca acccgaagct gggcaaggcg ctggtgggtg cctggtatga
      721 aacgctggcg ctcatgctgg ccaaggatgc caaggcaacc gcggcgctcg aatcgatggc
      781 caaggcgtcg ggaaccgacc tggccggctt caaaggacaa ttggcgacca cgttcctgta
      841 ttcgaccccg gcgagcgcgg tggccgccac gaaggcgccc gatctcgtca agaagatgga
      901 cctggtccgc aagttctcgt tcgaccacgg cttgttaggc gagggcgcga agaccgtcga
      961 tgcggtcggc atcgaattcc cgggcggagt cacgctcggc aacaaggcca atctcaagct
     1021 tcgcttcgat ctcggctaca tgcagatggc tctcgacggc aagctctgaa gcgcctttct
     1081 tgcgtcggct gatcaatcaa cacccggggc gggccgctgg gcttgccctg ggtgtgttgc
     1141 cgttcatcgc gttgctggtg atgtacgtca tcgcctcgca cctgcggctc gccgaaaacc
     1201 cggacgacaa actgctgccc gcgctgggca agatcgccag cacctttcac agttacgcct
     1261 tcgaagagga cagccgcacg ggacgcgtgc ttttctgggc cgacacgtgg gccagcctca
     1321 agcgaatcgg ctcggcgctg ctcatcagcg ccacgctggg aatgatcctg gcggttggca
     1381 tcggcgtgat ccccagagtg cgggccttgc tctcgccgct gctcggcacc atcgccatga
     1441 ttccaccgct ggcgatcctg ccggtgctgt tcatagttct cgggctcgag gagaccgcga
     1501 agatctcgct catcgtcatc ggcatcgcgc cgtgtatcgc gcgcgacctg gcgttgcgcg
     1561 tcggcgaatt gccgaaggag cagatcatca aggcgcagac gctgggcgcg tcgacctggc
     1621 aactcatcct gcgcgtggtg ctgccgcagc tgtggccgcg gttgatagat tccttgcggc
     1681 tgtcgatggg ctccgcgtgg ttgttcctca tcgccgccga agccatcgcg tcgactgaag
     1741 gactcggcta tcgcattttc ctcgtgcggc gttacctcgc catggacgtg atacttccct
     1801 acgtggtgtg gatcacgctg ctggcggtgc tcaccgacct cgcgctgcga ctgatccgca
     1861 cgcacgcctt ccgctgggcg gagccggagc gctgatggcc gccgtcaccg tacgcaatgt
     1921 ctggaaggaa tatggcgcgc aggtggtgct cgaaaacgtg cgcctggaaa tcgccgacca
     1981 cgagttcgtg acgattgtcg gggcctccgg ctgtggcaag accacgttcc tcaagatgtt
     2041 gttaggcatg gagcagccga cgcgcgggca gatcctcatc gacggtgtgc cgatccggcc
     2101 ggaaccggac ccggaccgcg gtgtcgtttt ccagcggtac tccgcgttcc cgcacctgac
     2161 cgtgatcgac aacgtgatgc tgggtctcga gctgcgctct tcgaagatca ccggcaggtt
     2221 gttcggcgca gcgcggcgcg aagcgcgcgc cgccgcgatg gccatgctcg agtcggtggg
     2281 tctcgcgcat gccgccgacc gctacccggc gcaactctcc ggcggcatgc agcagcgcat
     2341 gtcgatcgcc caggccgtga tccgcaaacc caaggtcctg ctgctcgacg agcctttcgg
     2401 tgccctcgac cccggcgtca cggccgatat gcacgaacta atcctgcggt tgtgggaaga
     2461 aaaccgcatg actatcttca tggtttcgca cgacatccag gagagcttcc agctcggtac
     2521 gcgactgctc acgttcgaca agctgcggca cgacccgcag tcgccggagg cctacggcgc
     2581 gagcgtgacc tacgacatgc cgctctcgcg ccgccgcccg cgcgctgcgc cgtcgtcctg
     2641 acgaaaaagt tcaaggcacg ctttcgcagg ccgcgccgct gttgttgttc tcgtcgaatt
     2701 cctttacggc tccatttcta tccgccgtca tcgtgacgac gaacggcttg gggcgttgcg
     2761 ccttaggcaa ctcaaaactc agcgtggtgg cgttctgcga gatcccgccg gggggaaggt
     2821 catattggag gaagtcttct tccccgcgca cggtcagtcc cttgttccat cgatgcatgc
     2881 cgacgaccag caccggcgcc gtacgcacgc cgttgatgat tggcagcgtt tgtatccagc
     2941 gcgtggcgcc gttgtccatg atcacgatga tgtccgggtc attgcgcaca tcgggggagt
     3001 cgatgtcggt gcggcactgg gatgtcaccg gatccttcac ggcgtcccac gcgaagatac
     3061 tggtttcgac aggcgggatg ctcgcgaggt tggtgctgcc gccattcctg gtttgcgccc
     3121 tgaatatcgc gtgggtcgag ctcgaatcat cggcgatgtt ttgcgcattg agatccagcg
     3181 tggacatcca tagcgggcgg ggcgcggaga aaacgcagtt cgccgccacg gcatttcggc
     3241 acgcacccac cgcccagttt gtgtcgacgt tgagcaagct gtcggcgatc gacccggccg
     3301 gcatgtcgta ggtgtatcga tagccgatgg ccagcgagcc gtcgtagacg gtaacgtgtg
     3361 gcagcgcgcc caccggaagg aagtcagccg gaccaaaggt gcagttcgca gcctgactcg
     3421 caagcttcac gcacaccaga aaatgggtgg gcagtggctg gctgggcgtg ctgaacaacc
     3481 cgtactcgac ccatgcgaat cgaacggtcg tcaatgcagg tacctcgccc ttcacggttg
     3541 cgccggccgc gggcgtcgac agcacgggcg tcggcagcag tgccgacggt ggaaaaacga
     3601 tctgcgcgct cgccgcggag ggcagcgtga gcgcaacgac taacaaaggg aacttgcggt
     3661 acatggttca ggtcctcgtt tatcaattta tgaacagcgt caccgtccgg ctggaggtga
     3721 gattcacggt gcattgggtg ccgtcggcat tcagggaggt gcagccgacc cagtcgccga
     3781 gcagcgaccc cgagtaggcg gacgcggtca gcacgacgac cgtgtcggcg gcgaactgcc
     3841 gcacgcaggt gggctcgctg tccttggaac aggcggtgac cacgggcacg taggtgacga
     3901 cgccgtgccc gccgccgcgc agcatcaacg tgagatccag cgcgccgggt gcggaggtca
     3961 ccgtcagcgt gcgcgtcgtc tgatcgctga gtcctccggc gtcggttacc gtgagcctcg
     4021 ctagataggt tccggcggcg gcgtaggtga attgccgcac gcgcgcgttg gcgtagatgc
     4081 cctctgcgtc ggttacgcca tcaccgtcga aatcccagcg atagatgcct atgcccacat
     4141 cgtcggtgct gctggtggca tcgaagctca ccgcgttgcc ggtctgcggg ctgccgggcg
     4201 aatacgtgaa gctcgcgacc ggtgggttgt tgctcgccac caccgtgacc tccacttcgt
     4261 ccgtgtccct ggcgccggtc gagtcggtga cggcgagtcg cacgcggtag agacccggcg
     4321 cgctccacgc atacgtggcc tccaccgcgg tggaatcgac gctgccgttt tcgtcgaaat
     4381 cccattgata ggtgagctgc gtgttgccgc cgtcgtcggt gctggaactg gcagaaaagc
     4441 caacagcttg acccacgcca acgctgcccg cggcgtcact cgtgatgacg gcaaccggca
     4501 cggcgttcac ggtgccaaag ttgaagacca cgccgcgatc gcgatccatg agcactcggc
     4561 aggtggccgc cgccgttgca gtggctggcg tgaacggatc gcagccttcg ttcagagtga
     4621 gcgtgttgtt gccgttgggc acgatggtga gcagcacttc atcggcgatg tcgtagatga
     4681 tctggcaggc attgccgccc ggcacgcagg gtggaatgtc ggtcgggcga ttcgcggtca
     4741 cggttcccgc gccgctgtcg tagggaaagc tgatggcgag ctggcgaatc ccgtccgaac
     4801 cgatgccaat gccggagaag aatgccacca cattgcgagt gcgttgcatg cccacgtgac
     4861 acacggtctg gccaaacgtg ccttgcggat ccagcgtttc ggcatcgcag cccgaccatc
     4921 cggacagata tcgattgatg cccggctcgg catgcagcag cacgatgctg tggcgcgcga
     4981 aaggccggtc gcaatccgcg gtgcagtcca tggagccgcc ggcggtgggt gggaccgcga
     5041 tcacgcgacc tgggatgccg atgccggtga gtgtgtccga caccaggcgc atctgcaggc
     5101 gctcgatatt ggtggcgaag cggccctggc aactcacgtc cgtggtgatg gcaaattcca
     5161 cggaacgcgc gccgggtacg tccgcgaggc cggtgcaggt gaaggtctcg aagctgtagt
     5221 tagcgtcggt ggatatgcct tcgaggcgca ccgtgctgcc ggtggcgaac gtctgcgtgc
     5281 aatccgcgcc catcgagccg caggtgattc cgacgggtac gctgcgcacg tagcccacct
     5341 gtggccccgc gcccgcggga ctcaaggtca ccgtcacccg cggcgagccc ggcggcccgc
     5401 ccgtgaactc ggccgagcag gtgaggttgc ccgtcatctc gagattgatc ggcgagccgc
     5461 gcgcgaaggc ggcgcaggcg ccgccccagc cgacgaaagt ctcagtggcg ctggcggtgg
     5521 cgctgagtgt gaggatctcg ccgggagaat aggtgccgcc gcaggtaccg ctgctgccgt
     5581 tgctgcagtc gaggcccgcg ccgctgacac ggccattgcc ggtaacgttc agtgtgaaac
     5641 gcggcgcgtt ggcggcgcgg aaagtggccg tgactgtgcg atcggcggtc atggtcaata
     5701 tgcagctgta ggcggtcgcg atatcgcacc cggtgaaacc caggaaacgg ccgcgctcgc
     5761 cgctgtcgac cgcgagggtc acggtggtat tcggtagata ggccgcctcg caccgattcg
     5821 cacccgttcc gcaggagatg ccgcccggtg cgctggtgac gactccgggc gaactgacat
     5881 tgccctgcac gacattcagt cggtggcgca gcggcggtgt gccgcccgcg tccggctggg
     5941 tgaaccccgc acactggcgt gccactgcgg gcaccggaat cagttgtgtc ggcacggcgc
     6001 tggcgcgcga ggcggcgttg ccggcgatgc gccgccccgc agcggtgctg cggtctcgca
     6061 tcccgagcgt cagcggggcg tagttagtcg ccagctcggc ggttcgcagg gcattgcagc
     6121 cctcgttgat acggccgtca ggaccgagtc gcatgaccca cgcttcgcca cgctcgccga
     6181 gcgagtcgga gcggccggca actacgaagc cgccgtcggc gagcgcgtcg aggccgaaca
     6241 gggtttcgtc atacaggccg ccataggtcc gatgccagcg cgcggcgcca tcggcggcga
     6301 gacggatcaa caggatgttc ctccggccgg cggcggccag ggcgctctcg tcgttgaagg
     6361 cgccgccgtc gagatgactc gatgccagca ggaatccgcc gtccgcgagc gtcacgatgc
     6421 gcgccagcgg tccgtacagg gagccaccca cctcgaagga gtacgaccac tcgagggcac
     6481 ccgcggcgga gatgcgtgcg gcccatggaa gctgcatcgc ctgaccgccg gcgacaccgg
     6541 cgacgatgac gccgccgtcg ctggtcacgt cgatgccgga aagcgcgccg ccaatgggca
     6601 actggcgctg ccagacgatg ccgcccgtgg catcgatacg cgtgacccac ggggccgcgc
     6661 tttggatgaa ctccaggtct tcgccgatga cgtagtaacc gccatcgctg cgggcgacga
     6721 tgccggtggc gatcggctgg ggcgaggggt tggggttgag cggcgccgtg ctccacaaga
     6781 cggcgccggt ggcgtctagc ttgatcactc gcggctgcgg agcgacgcct tcagtggcac
     6841 tgactccggc gagcacgacg ccgccgtccg tgctcgccgc gatggcgcca tagggcaggt
     6901 gcttgcggct caacaggctg aagttgtcga ggcgcacctg ccatagaaca tcgccgacat
     6961 tggtgtcgag gcgcagcttg gcgactacgg tgccgggcag ctctacatcg ccggtcgaat
     7021 cgctctggcc cgcgacgacg aaaccattgc tcaactcgac cgtggcgaag gcgcgttcgc
     7081 cgcgaaattc cgagcccgct ccaaagttgc gatcgaccag cggctcgccg gcattgccga
     7141 atacctggat gtgggcgcga tctccgggtt ggtagacaga caccacgcgc gcgtcgcggt
     7201 cgaactgcag cgcgggtagc gggtaggttt caccatcggc ggggccgtac gccgcggagt
     7261 cacgcgatac ttcggtgagg ttcgggcgga tcagcatccg gaacgtgccg ccgtcggacg
     7321 tatatcccac cgcctgcaac ggcgtgttgg catcggcggc gcccggcgcc gcgatgcgca
     7381 ggtcggagag gatttcgagg ccgtcgtaga actcagtcgt gaacaaatcg ccgtcgggtt
     7441 cgacgtaggc cgacatggcg agcagcgcac cttcgtcgtc ccggctcccg cgtccgcctg
     7501 ccacgtagtg gcaatagcgc gccagggagg gcgtggggcg gcacatctgc accacggttt
     7561 cgaacagcgc gccttcctcg atgaccgagc gccactcttc gtcgccgtcc tcgcgcgaaa
     7621 acttcatgac gatgctgtcg cgatcgccat cgagggtgta ccagatggcg atgtcgtctc
     7681 caacgatcac gaagccgtcc tcggcgccgc cgtcgaacac gtcgtcgtcg ttggcgtctt
     7741 ccacctggat gagatcgcgt gcgatcagtc ggctataacg gcgggtccac ggcaccccgc
     7801 ccccatcatc gagcagcgac agctgcatcg caggtttggg accctgttca gtgctgtcgc
     7861 cacgaacacg tgcgctggcc gcaaccaccg cgccgttctg cgccgtcgcg cgcaccagca
     7921 gctccgccgg ttcctccggt agatagtcat actgcgtttc gctgatgcgc cggctccact
     7981 gcacgatgcc ggtcgcgttg actttgacga tccacggcgc cgcgaccggg atgccgttgc
     8041 tcgcatcgac tcgcaccacc gcgctgctcc aggcggcaag cagcgcgccg ccgtccggcg
     8101 tcggccacac gtcccagccg gtgtcgcgtg cggattcgtt ggcgcggaag aattcgcgcg
     8161 aggcaaagcc gccgccgtcg tagcttcggg tccagcgcac gctgccgtca tcgttcagac
     8221 gcgcgaccag cagatcgaca accgcatccg gattgctgac atcacccgag gggccggggc
     8281 cgacctgggt gccaaccatc cagtaaccat cgtcgcccgc gcgcacgcgt ttgaagagcg
     8341 cttcggcgcc cgcggccgcg gcgcgcttct cgcccagcgc gagttgccag tcgaggtcgc
     8401 cggcctgatc gagcttcgtc acccagaaat cgccgtcgat cggcggaagg cctgactcat
     8461 cggtttgctg atgatcgaat acgccggcta acagaaagcc tccgtcgcgc gtggcgacca
     8521 cggcgttggc ccagtcgtct cccgggccgc cgatcgactt cgaccaggtt gccgctgcgg
     8581 gcggagtcgt cggatcgtcg tcatccggag gtaggccggg cgatccggag caggcgctga
     8641 gcgtgagggc gaataacgag gcaaaaaatg cgcgtgaacg atcgaagatt ggcatggtgg
     8701 gcttccgtga cacgaagaaa ggcttcttgg gttacggaaa cggcacggcg aatgggccgc
     8761 gaagggccaa ttatttcgaa cctgtaaaaa gtatcgacgc cgtcgcgtca gttcgcggtg
     8821 tctcgcagca ctttctgatc gactggcagc ggagatttcg ccaacagcgc gcgcgcctcc
     8881 gcggtgcgct tgctcgcagc ttggcgttgg ccggcggcga actcacagcg cgcgagccag
     8941 aggttggcga gcgccagctc gcgcgacggg ccgttcaacg tgcgccagta gtcgcgcgcc
     9001 tcctgggcgt aaatcatggc gttcgcgaac tctttctcgt gcagccgtac gcgcgccata
     9061 cccaacaggg catccgcgcg tatcggcgaa agctgcgtct gctcgcgctg cgtggtcgca
     9121 agcgcctcgg tgagcgccgc tgtcgccgag gcgtagtcgc cggcgtcgag cgccgccagg
     9181 ccgagatccg tgacgattgc catgcgccgg aattttgcac gcgggtcatc ggcgagcgcg
     9241 gcgagcgcgg cgagggcggc gcgttgttgc gccatcgccg cgaccggctg cgccgccaga
     9301 cgctgcacga tgccgagagc gtgtggggcg gagacgctca tccagtgatc gagtttgcgg
     9361 ccggacgcct gcagtttgcg cagcgccgtt tctgccgccg cgatctcgcc gagcgccgcg
     9421 cgtgcaagtt gaaaattggc gtcgacgacc gcagtggtgt cgtgatccgg tccgatcgtc
     9481 gccttgagga tgtccagcga ttgctcgaag gcctcgtgcg cctcatgagg gcggcgtgcg
     9541 agaagcaggg tgcggccgcg cgtcgcgagc gccgccccag tcgtgtacga gcgtggctcg
     9601 ttccactttc gtatcagtgt gagggatcgt tcgctgttgc ggagcgcgcc ttgcatgtcg
     9661 cctgtgtcga gttgggcccg ggccaggaac gaagacgcga atccggagag catgctgtct
     9721 tcgccgagca ctgcgaccgc atcgaccagc acgcgctgca gttcgttgac ggcctccgcg
     9781 ggctgccccg cctggatgag ggtgccaccg taagtcatgc gcgcgtcgac gaccatcgca
     9841 tgtttctgat tgccgtcgac cagtgtcaac gacaaccgca gggcttcgcg cgccgcatct
     9901 acggcctcga gcggccgccc ggcgctgcga aaggcaattg ccctgactac ggcggcgcca
     9961 acggtgagct ggttgcgttc acccagcgtc tgcagtgcaa ggggcagcgc ttcattcatg
    10021 gcgcgaatcg caagctcggg tctgccctcg tcgatcgctg cgtgcgcatc gaatacgagc
    10081 agcctgatga gttcggtcgg ggtggtgtcc ttgctggcgc gggcggcggc cagcaactcg
    10141 tcgacttcct tgcgcaactg cgccgtgcgg cctcgcgtgc gctcgatgtc gaggcgcacc
    10201 atgcgtgcct cgcgcgtgag gggatggcga tcgccaaacg ccacgcggga aatcgccagc
    10261 gcctcattgg tgatctgctc ggcgctgtca tagtcgtcga ggtttgcgag gcttgccgca
    10321 acggtggtca gcagctcgac gcgaatctcc gggcgattca cgaagtcgcg atcgatgcgg
    10381 gaacgtgcct gttgcagcag tgcctgggca gtcaccgagc ggccaccgcc ttcctgcggg
    10441 tccgcgttca ggaagatcgt ggcgatgaat ctcttgatgt cctcggctcg atgttgttcg
    10501 tcgcgtgcca gctgcatctg ccggagcgca gttccggtgg ctcccagcag cgccgccacc
    10561 accatcgccg ttaccgcggt ggcgagtttg tgacgcttga gaaaccggcc aatgcgatat
    10621 agcgcgcgat ccggctgcgc ggataccggc cggccggcga ggtggcgcgc gatgtcgtcg
    10681 gcgaacgcat gcaccgtggc gtatcgccct tcgggacgct tgtgcagcgc cttgagcacg
    10741 atcgcatcga ggtcgccgcg catcgcacgc cgcgcaactg cgtgggctgc tgcgtcgctc
    10801 gggcggcgtg cttcggcttc caggatcgct tcctcgagcg cagctcgtga ttcacgcttc
    10861 agccgataag gtcgcgagcc ggtcagcagc tcgtagagca cgacgcctaa cgaataaaca
    10921 tcggtggcca ccgtaattgg cagcccggcg atctgttcgg gggacgcgta gtcaggcgtg
    10981 aatacacggg ccgacacctg ggtgagctgg gcgtcagagc cggcttcgtc gtccagtagt
    11041 ttggcgatgc cgaagtcgag cagtcgtacc tggccatcgt gggtgaccag gatgttcgcc
    11101 ggcttgaggt cgcgatgcac cacgagtttg ccgtgcgcgt aggccacggc gtgcgccacc
    11161 tgcgcgaaga gttgcaggcg ctgccgtgtg tcgcagcgct tctccgcgca gtagtgatcc
    11221 acgcgcacac catccacgaa ctcgagcgcg atgtaaggct ggccatcgtc cgtcatgccc
    11281 gcgtcgtaca accgtgcgat gttcggatga tcgagactcg cgagaatgcg gcgctcgcgt
    11341 gccatgcgtt ccaccaggcg cgcaccgaac agatcgcgat gcggtagctt gagcgcgacg
    11401 ccgcgttcaa acaggccgtc ggcgcgctgc gcgagccaca ctgtgcccat accgcccgtg
    11461 ccgatcggtc gaaccaggcg gtagggtccg atgcgttcgc c
//
LOCUS       DASQJS010000437         6565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1539655, whole genome shotgun sequence.
ACCESSION   DASQJS010000437 DASQJS010000000
VERSION     DASQJS010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539655"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1631)
                     /locus_tag="VEW08_17250"
     CDS             complement(159..1631)
                     /locus_tag="VEW08_17250"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HYJ42541.1"
                     /translation="MSRRFNLLSFRSLLLSVIAACVSSHAFAQTPVRIMPLGDSITAG
                     PGCWRAYLWNQLQTAGYSNIDFVGGVNDGGGCNPGFTYDFDHEGHSGFSITGIADGNQ
                     LPPWLTAARPDIIVMHLGTNDMWGGWIPLETKITALTKLVGQMRANNPNVRIVASKII
                     PMSPSGCTTCAADVVAFNNAIPGWAAGLNTAQSPIYVVDQWTGFDVVADTYDQVHPLT
                     SGFIKMANRFFPVVSQALNAGTPGIALTVTKAGAGTGAVTSSPAGINCGATCMANYTS
                     GTAVTLTATVASNSTFAGWSGACTGTGTCTVSMTQARAVTATFNAMATFSLSVSKAGT
                     GSGTVTSSPAGINCGTTCVASFGGNASVTLTAAAASGSAFAGWSGACTGTGSCVLAMN
                     QVRAVTATFNTSGGTGGTCANPATFSGNSGNFNTTGAVCYRTNATVSGWGCYNFDGRT
                     VTVGGVARTCGQLPLTRAADGYYYFAVTAGQYPWAGLFTW"
     gene            1899..2162
                     /locus_tag="VEW08_17255"
     CDS             1899..2162
                     /locus_tag="VEW08_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42542.1"
                     /translation="MQDQKKEHGSRHRRASEIIASRIVSVRSSNTEPARVEVETDSGR
                     HELTVPVESTDLRWLLIRLACSDKRIRLAVTDEAQIEAVEAVY"
     gene            2294..3058
                     /locus_tag="VEW08_17260"
     CDS             2294..3058
                     /locus_tag="VEW08_17260"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYJ42543.1"
                     /translation="MRIFITGSTDGLGRAAALKLINEGHQVVLHARSKKRASALADLA
                     PSSAGVAVGDLALAAETRAIADQVNKIGRMHAVIHNAGIYRESSRGTTPEGHAKVLAV
                     NTLAPYMLTKLIERPERLIYLSSGMHRGGEDSLRDIDWSDRRWDTNQAYSDSKLYVTA
                     LAMAVARKWPKTVSSAVDPGWVPTKMGGPGAPDDLEQGHLTQTWLATSDEPAAKVSGG
                     FWFHRKQQKPAAAALDVAFQDRLIARLTELTGLALF"
     gene            3055..4293
                     /locus_tag="VEW08_17265"
     CDS             3055..4293
                     /locus_tag="VEW08_17265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYJ42544.1"
                     /translation="MTTDVDVAIVGGGAAGIGAARRLAQSNYSTVLFEAGPRLGGRAW
                     THEVSGLHLDLGCAWFHSAERNSWIRIAEAAGIPIDRRPAQWGNQYRDLGFPKAERVK
                     AGEAFAAWMHRLESSPSASDCAADALAPECEWNDYIRTIVGFISGGDPGQLSIADYLA
                     YDQSSSENNWRVPSGYGSLVANSFPSRVPFHLATPVESIALEASGVTLATPAGTVRAR
                     AAILTVSTAVLAGDVLKLPAELTPWMEAASCLPLGRNEKLFFEIVGDAPFADESQLLG
                     NPRQRTASYYIRPLGSRVIECFFGGEGARFLEESGHAAGFDFALGQLSALFGAGIRGS
                     LRPLIGTRWSEAKHIGGGYSYALPGHATARTALARPFDERLFFAGEATSAGDFSTAHG
                     AHDSGVRAAEEAIAAILRAP"
     gene            4312..6162
                     /locus_tag="VEW08_17270"
     CDS             4312..6162
                     /locus_tag="VEW08_17270"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HYJ42545.1"
                     /translation="MTCNESGRKTIARFALCVVLAMVTTSALADVAALSVNGNQVLAG
                     GKPASFAGNSLFWSNTGWGGEKFYNASVVSWLKRDWKADIVRAAMGVEAPGGYLQDPK
                     GNLARVKAVVDAAITNDMYVIIDWHSHHAESQRSEAITFFQQMAHAYGTHKHVIYEIY
                     NEPLKISWSGKVKPYAEAVIHAIRAIDPDNLIVVGTPTWSQDVDVAAADRIAGANIAY
                     ALHFYAGSHGKSLRDKAQQALDRGVALFVTEWGAVNSNGDGSPDFKETSAWMNFLETR
                     GISHANWATNDKAEGASALVRGASTEGGWPANQLTASGSLARKIISAWGSAPLPDDEL
                     VLNVPGYADFIAVDGESVWVTNEGRVERWSAKGKIASVTMPKPCGAMSVAFGSLWVAD
                     CTDLAVYRIDIRTAKTLAVIRTGIADPEGETNVVAGDNSIWVPSAASGKISRIDPSTN
                     LVTALVTVDAGTSYLAYGFDSLWAVSSQGATLQQIDLANNQVARRVALGRQPGFLAAG
                     EGGVWVQEQGDGTVARVDPGTAAVTGRVKVGDNLKYGDIDTGDGKVWLRTTADQTFVV
                     IDPKSMSVLTRIGKPSGSGGLRYTPLGVWTSAHDLHTLSLWKTADQAKSR"
     gene            complement(6367..>6565)
                     /locus_tag="VEW08_17275"
     CDS             complement(6367..>6565)
                     /locus_tag="VEW08_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42546.1"
                     /translation="DEIPRPATQISAAILLRLTIMKIPLRVDSIQNDHARGHRCLSAA
                     AMVAGVGMGYESLVMCASSSP"
BASE COUNT         1252 a         2005 c         2125 g         1183 t
ORIGIN      
        1 aaggtctgcg aagtgacatg ggttccccct gataagttga tcattccatc gatcgtctgg
       61 cagcagggca gagcccccac tcttccgctt gccgaccggc gattctatgc gacgacgtcg
      121 cacgaaacgc aagccggagc ggactaacaa agtcaaaatc accaggtgaa caggccggcc
      181 cacggatact ggccagcggt caccgcgaag tagtagtacc catccgccgc gcgtgtcagc
      241 ggtagctgac cgcacgtacg cgcaacgccg cccacggtca cggtgcgccc atcgaagttg
      301 tagcagcccc atccactcac agtcgcgttg gtgcgatagc aaaccgcgcc ggtggtgttg
      361 aagttgcccg agttgcccga gaacgtagcg gggttggcgc aggttccgcc ggtgccgccg
      421 ctggtgttga acgtggcagt cacggcgcgc acctggttca tcgccagcac gcaactaccc
      481 gtgcccgtgc atgcgccgct ccagcccgcg aacgccgagc cactcgcggc cgcagcggtc
      541 agggtgaccg atgcattgcc accgaagctg gcaacacagg tggtgccgca gttgatgccc
      601 gccggcgaag acgtgacagt tccgctgccg gtgccggcct tcgacacgct gagcgagaag
      661 gtagccatgg cgttgaatgt ggcggtcact gcgcgcgcct gcgtcatcga caccgtgcag
      721 gtgccggtgc cggtgcacgc gccgctccag cccgcgaacg tcgagttgct ggccacggtg
      781 gcggtgagcg tcacggccgt gccgctcgtg tagttagcca tgcaggtggc gccgcagttg
      841 atgccggcgg gactcgatgt gacggcgccg gttcccgcgc cggccttggt gacggtcagg
      901 gcgattcccg gggtcccggc attgagcgcc tgcgatacca cggggaagaa ccggttcgcc
      961 atcttgatga agccggaggt gagcgggtgt acctggtcgt aggtgtccgc caccacatcg
     1021 aagcccgtcc actgatccac gacgtagatg ggcgactgcg cggtattgag tccggcagcc
     1081 cagcccggaa tcgcgttgtt gaacgcgacc acgtccgcgg cgcaggtggt gcagcccgac
     1141 gggctcatcg ggatgatctt tgacgcgacg atcctgacgt tcgggttgtt agcccgcatc
     1201 tggcccacga gtttcgtgag cgccgtgatc ttggtttcga gcgggatcca gccgccccac
     1261 atgtcgttgg tgccgaggtg catcacgatg atgtccgggc gcgccgcggt cagccacggc
     1321 ggtagctggt tgccatccgc aatgccggtg atcgagaaac cgctgtgacc ctcatggtcg
     1381 aagtcgtacg tgaacccggg gttgcagccg ccgccgtcat tcacgccgcc gacaaaatcg
     1441 atgttcgagt agccggcggt ttgcagctga ttccacagat acgcgcgcca gcagcctggt
     1501 cccgcggtga tggaatcgcc cagcggcatt atgcgcactg gcgtctgtgc gaacgcgtgc
     1561 gacgagacgc acgccgcgat cacagacagc aacaacgagc ggaatgaaag caggttgaat
     1621 ctgcgagaca tgagacctcc ttgtatttgg cgcggcgcaa tgcgccggcc agtcctgcag
     1681 cggcatcttg ccgctggatt cgcgctggat gatgagccgg aagtgattgg tttggaaggc
     1741 gaatctgcgc ggcgagcatt gtcctgcgag ggcatcggtc attggcgagt catgcgcggc
     1801 ctgcaccggc gggcagaaga attcccatcg cctctgtaac cgccagcgcc ctggtgtcag
     1861 ggtatgaata ggggcaagtc gcgcttggag tcgcacaaat gcaggaccag aagaaagaac
     1921 atggttcgcg gcaccgccgt gcgtcggaga taatcgcatc ccgaatcgtc tcggttcgga
     1981 gctcgaacac cgaacctgcg cgcgtcgaag tggaaaccga cagcggtagg cacgagctca
     2041 ccgttcccgt ggagtcgacc gatttgcgat ggttgctgat tcggcttgcc tgcagcgaca
     2101 aacggattcg gcttgccgtc accgatgagg cgcagatcga agccgtggag gccgtgtact
     2161 agaagcccgc ggggcggaac tttccaggca gcaggtcatc tctcttggca tgatcgaatc
     2221 gcccgcccgc cgccgcctgc tctatctcgc tgcaatggcc gcggcggggg tggcaagcgg
     2281 aaaggatcgt tccatgcgca ttttcatcac gggctctacc gacgggcttg gccgtgccgc
     2341 ggcgctcaag ctcatcaacg aaggccacca ggtggtgctg cacgcgcgct cgaagaaacg
     2401 ggcgtcggca ttggcggatc tcgcgccgag ttcggcgggc gtcgccgttg gggatctcgc
     2461 gctcgcggcg gaaacgaggg cgatcgcaga tcaggtcaac aagatcggac gcatgcacgc
     2521 cgtcattcac aacgcgggca tctaccgcga gtcaagccgt ggtacgacgc ccgaagggca
     2581 cgcgaaagtt ctcgccgtga acacgctggc gccatacatg ttgaccaagc tcatcgagcg
     2641 accggagcgc ctgatctacc tgagcagcgg catgcatcgc ggcggagaag actcgctgcg
     2701 cgacatcgat tggtccgatc ggcgctggga taccaaccag gcgtactcgg acagcaagct
     2761 ctacgtcacc gcgctcgcca tggcagtcgc cagaaagtgg ccgaagaccg tgagcagcgc
     2821 cgtggatccg ggctgggtgc ccaccaaaat gggagggccc ggtgcgccgg acgatctcga
     2881 gcagggacat ctgacacaga cctggctcgc taccagcgac gagccggccg cgaaagtcag
     2941 cggtgggttc tggtttcacc gcaagcagca gaaaccggcc gcggctgcgc tggatgttgc
     3001 ctttcaggat cggctcatcg ccaggttgac tgagctcacc ggcctcgcgc tattttgact
     3061 accgacgtcg atgtcgcaat cgtgggtggc ggtgctgccg gaataggcgc cgcgcgccgg
     3121 ctcgcgcagt caaactactc gactgtcctg ttcgaagctg gcccgcgcct cggtggtcgc
     3181 gcctggactc acgaagtttc cggtttgcat ctcgatcttg gctgcgcctg gttccattcc
     3241 gccgagcgca actcgtggat caggatcgcg gaggccgcgg gaattccgat cgaccgccgg
     3301 ccggcccaat ggggcaacca gtatcgtgac cttggcttcc cgaaagcgga gcgggtcaag
     3361 gccggcgagg cgtttgcggc ctggatgcac cggctcgaaa gctcaccttc agccagcgat
     3421 tgcgcagccg atgcactggc tccggagtgc gagtggaacg actacatccg caccatcgtc
     3481 gggttcatca gtggcggaga tccggggcag ctctcgattg ccgactacct tgcctacgac
     3541 caatcgtcat ctgaaaacaa ctggcgggtg ccatccggct atggatcgct ggtggccaac
     3601 agctttccat cgcgggtgcc gtttcacctc gcgactcccg tggagtcgat cgcgctggaa
     3661 gccagcggag tcactttggc cacgcctgcg ggcaccgtgc gagcacgggc cgcgattctc
     3721 actgtctcaa ccgccgtgct ggccggcgat gtcctgaagc ttcctgccga gctcacgcca
     3781 tggatggagg ccgcgagttg cctgccgctg ggccgcaacg aaaaactgtt cttcgagatc
     3841 gtcggcgacg cacctttcgc cgacgaatcg cagttgttag gtaatccccg gcagcgcact
     3901 gcttcgtatt acatccggcc attggggtcg cgcgtgatcg agtgcttctt cgggggagaa
     3961 ggcgctcgct tcctggaaga gagtggccac gccgccggct tcgacttcgc tctcggacaa
     4021 ctgagcgcgt tattcggcgc gggcattcgc ggctcgctgc gtccgctgat cggaacccgc
     4081 tggagtgagg cgaagcacat tggtggcggc tatagctatg cgttgcccgg tcacgccacc
     4141 gcgcggacgg cgctcgcgcg gccgttcgac gagcgactgt tctttgcggg tgaggccacg
     4201 agcgccggcg atttctccac cgcacatggc gcgcatgaca gcggagtgcg cgctgcggag
     4261 gaagcgatcg ctgctatcct gcgcgcgcca taaacgagga aaggcgccgc catgacttgc
     4321 aacgaatccg gccggaagac cattgcgcga ttcgcgcttt gtgttgtcct ggcgatggtc
     4381 accacgtcag cgctcgcgga cgtcgccgct ctttcggtca acggaaacca ggtactcgcc
     4441 ggcggcaaac cagccagttt cgcaggcaat agtttgttct ggagcaacac cggctggggt
     4501 ggtgagaagt tctacaacgc gagcgtcgta agctggctga aacgcgactg gaaagccgac
     4561 atcgtccgcg cggccatggg tgtcgaggct cccggcggat atctgcagga ccccaaaggc
     4621 aaccttgcgc gtgtcaaagc cgtggtggac gccgccatca ccaacgacat gtacgtgatc
     4681 atcgattggc attcgcatca cgccgagtcg cagcgcagcg aggcaatcac gtttttccag
     4741 cagatggcgc atgcgtacgg cacgcacaaa cacgtcatct acgagatcta caacgagccg
     4801 ctgaaaattt cctggagcgg gaaggtcaag ccttatgccg aggcagtgat ccatgcgatt
     4861 cgcgcgatcg accctgacaa tctcatcgtc gtgggcacgc ccacctggtc gcaggatgtg
     4921 gacgtggcag cggcggaccg catcgcgggc gccaacattg cctatgcgct gcacttctac
     4981 gccggctcgc atggtaagtc actgcgcgac aaggcccagc aagcgctgga tcgaggtgtc
     5041 gcactctttg tcaccgagtg gggcgcagtg aattcgaatg gcgacggttc gcctgatttc
     5101 aaagagacca gcgcctggat gaatttcctc gagacgcgcg gtatcagtca tgccaattgg
     5161 gctaccaatg acaaggccga aggcgcgtcc gctctcgttc ggggtgcgag caccgagggt
     5221 ggctggccag cgaatcagct caccgcttct ggctcgcttg ccaggaaaat catatccgcc
     5281 tggggctccg ctccgctgcc cgacgatgaa ctcgtgctca acgtgcccgg ttacgccgac
     5341 ttcattgccg tggacggaga atcggtctgg gtcacgaatg aagggcgggt tgaacgatgg
     5401 tccgcgaaag ggaagattgc gtcggtcacg atgcccaagc catgcggcgc aatgtccgtc
     5461 gcgttcggtt cgctgtgggt ggccgactgc accgacctcg cggtctatcg aatcgacatt
     5521 cgaacggcga agactctggc cgtcattcgc accgggattg cagatccgga aggtgagacg
     5581 aacgtcgtcg ccggcgacaa ttcaatctgg gttccgagcg ccgcgtccgg gaaaatttcc
     5641 cgcatcgatc cctccaccaa tctcgtgacg gccttggtaa cggtggatgc cggcacgtcg
     5701 tatctggcat acggtttcga ctcgctctgg gcggtcagca gccaaggcgc gactcttcag
     5761 cagatcgacc tggctaacaa ccaggttgcg cgtcgcgttg cgctcggacg ccagccaggg
     5821 ttcctagcgg cgggagaagg gggcgtgtgg gttcaggagc agggcgacgg caccgtcgcg
     5881 cgtgtcgatc cgggcactgc ggctgtcacc ggccgcgtga aggtgggaga caacctcaag
     5941 tatggggata tcgacaccgg cgacggtaaa gtgtggctgc ggacgactgc cgatcaaacc
     6001 ttcgtcgtga tcgacccgaa atcgatgagc gtactgacgc gcattggaaa acccagcgga
     6061 agcggcgggc tgcgctacac accgctgggt gtatggacga gtgcgcacga cctccacacc
     6121 ttgtcgctct ggaaaacggc ggaccaggcc aaatcccgct gatcatgttg agtaccgctg
     6181 acaaccgcgt cagacgcggc cccaatccat ataggtagaa agcgcaaacc agccctggag
     6241 agctccggaa cgcttgactg tggtagcgct atcggcgatt actacctcgc cgcacaattc
     6301 gccgttccga caagggatga atgcgaatga ctaacaaaaa atagcggcaa gacaggaagt
     6361 cgagattcat ggggaggagg acgcgcacat gaccagggat tcatagccca ttcccacgcc
     6421 cgccaccatc gcggctgccg aaaggcagcg gtggccccgc gcgtggtcgt tctgaatcga
     6481 atcaacacga aggggaattt tcatgattgt tagtctcaaa aggatggcgg cgctgatctg
     6541 cgtcgctggt ctaggtattt catct
//
LOCUS       DASQJS010000438        10804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3114708, whole genome shotgun sequence.
ACCESSION   DASQJS010000438 DASQJS010000000
VERSION     DASQJS010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10804
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3114708"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VEW08_17280"
     CDS             complement(<1..584)
                     /locus_tag="VEW08_17280"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HYJ42547.1"
                     /translation="MSQVEATFSLLLVCPDRKGIVAAVANFLSDHDASIVEAKQFNDQ
                     LTRRFYMRAVFTAVGPKFIAIEDLRAKFATLASAYEMTWQIEDTVHRVRVLIAVSKLD
                     HCLQDLLFRWRSGVLPVDVVGVISNHPDLRSLVEWHQIPFHHLPVTAGTRAEQEAQIL
                     ELISRYKVDLLVLARYMQILSNDLCRTLSGRCIN"
     gene            complement(630..1334)
                     /locus_tag="VEW08_17285"
     CDS             complement(630..1334)
                     /locus_tag="VEW08_17285"
                     /inference="COORDINATES: protein motif:HMM:NF040452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HYJ42548.1"
                     /translation="MHRSTHKMLTVVFVLLLAVVASEALADGSRGHGPRGYGYGNPGY
                     NRYSYSHHGSYSNYRYNYPARGYATYALPRGYHTAHYRGSPYYYHGGSWYRPYGPRYV
                     VVAPPFGIGIGVLPPFYTTLWYGGSPYYYADNTYYSYRPERREYVVTEPPRGEPEGGS
                     EPATGEVFVYPKNGQGEEQQANDRYECHRWAVERTGFDPTRPAGNVAETDMTSKRADY
                     RRAEGACLEARGYSVK"
     gene            complement(1350..1931)
                     /locus_tag="VEW08_17290"
     CDS             complement(1350..1931)
                     /locus_tag="VEW08_17290"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HYJ42549.1"
                     /translation="MRGKHFQAVMTMAMFAAMAMAIAIAIAAPAQADDATAPSAPDAN
                     APRGADIYVYPTNGQSERQLDRDRYECHGWAGKQTGYDPSLQPLSSHQKLQVVAVPPD
                     HRGAVNGAVAGAVIGAAVSGPHDSAEGAVVGAVAGAVIGAATDSARAREAAGLASRQT
                     AAEQAEQARLEKQAYDYRRAITACLEGRGYTVK"
     gene            2197..3477
                     /locus_tag="VEW08_17295"
     CDS             2197..3477
                     /locus_tag="VEW08_17295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ42550.1"
                     /translation="MDDRLTARRTRARFFILALLTVGTLINYLDRTVISVAAPLMTKE
                     LGLSAVAMGVVFSAFSWTYAAAQIPGGILLDRIGVRLTYFLSVTIWSAFTLLQGLTIN
                     LWTLIACRMGLGVAEAPAFPSNSRVLGTWFPQSERASATGVYSIGQYFGLAFLSPLLF
                     WIAGTFGWRALLIIVGAVGILFGFVWYAFYRDPHESRANQAELDYISAGGGVPSSSGV
                     RFEWRHIAFLMRQRQILGASIGQFASNSTLVFFLTWFPTYLATERQMGWIKVGFFTVL
                     PFIAACVGVMTGGLCSDLLLRRTGSANVARKLPIIAGLLLASTIVIANFVTRDTLVII
                     VMSVAFFGQGMCNLGWTLLTDVAPKELMGLSAGVFNLCANLAGIVTPLVVGVVVGVTG
                     SFAWALAFIGIVALLGVFSYVFIVGDVRRVEYHA"
     gene            3474..4340
                     /locus_tag="VEW08_17300"
     CDS             3474..4340
                     /locus_tag="VEW08_17300"
                     /inference="COORDINATES: protein motif:HMM:NF009201.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HYJ42551.1"
                     /translation="MSEHRTLKVGLIGAGIQHSSSPSMHVDEGKALGLSITYELLDFD
                     LMQGGPNRLAALLDDAEQRGLRGLNITYPSKQAVIPLLHELAPEARALHSVNTVLFRN
                     GKRIGHNTDWWGFAESFKRDMSDVNTDHVVLVGAGGAGAAVGYAMLKHGSKSLTIHDM
                     DGTRAAALVDRMSELFPDRSIDVCRDLPASLARASGLIHATPTGMRKHPGIPVPAESL
                     RPPLWVSEIVYVPLLTELVVTARKHGCRTLDGGGMAVFQGAMAFKLFFDVEPDIARML
                     RRFHSQLSTVAA"
     gene            4349..4801
                     /gene="aroQ"
                     /locus_tag="VEW08_17305"
     CDS             4349..4801
                     /gene="aroQ"
                     /locus_tag="VEW08_17305"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HYJ42552.1"
                     /translation="MRILVLHGINLNMFGKREASVYGTTTLAEIDDALKVLARELGAE
                     LEFFQTNHEGEICERIHRVVIEKNMGAVMINAGAWTHYSYGLRDALAMLTAPIVEVHM
                     SNIHAREAFRTHSVIAPIAKGQVCGFGVESYLLGLRAAVSLARQKPAN"
     gene            4844..6289
                     /locus_tag="VEW08_17310"
     CDS             4844..6289
                     /locus_tag="VEW08_17310"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYJ42553.1"
                     /translation="MYEPVGGIDSEGVVRQLQSDPDHAALDAMILRARAAMSEVAGYD
                     QARIDRLCQALGWATANEKTFNRIVAMSIEESRLGDPEGRKSKRMKILGVLRDALRQK
                     STGIIEQIPERGIVKYAKPAGVIASLVPTTNPELTPPYTGIYALKCLDAVIFSPHPRS
                     KRTTFEMVRVMREAIRRQGAPADLFQCIENPSVPLTQYLMANCDLTLATGGQPMVRAA
                     YSSGRPAYGVGAGNSTMVIDETADIDIAARNTRMSKTSDFGSGCSADGNILIDSTIYH
                     AFVAALVREGGFLASEEQAAQIEKVMWDDHGHRLPETVACPPQAITRAAGFEISADRQ
                     FIIVENDRIGPRANYSREKLTTLLAVYRYEGFDEALRMVRKIYEVGGKGHSCGIYSHD
                     DDHIDRLARVAPVSRMMVRQPQSKANAGSFTNGMPMTSSLGCGIWGGNITNENIHLKH
                     FMQTTWVSRPIPEDRPSDQELFGEFFNTETL"
     gene            6289..8058
                     /locus_tag="VEW08_17315"
     CDS             6289..8058
                     /locus_tag="VEW08_17315"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYJ42554.1"
                     /translation="MKDTVANHLVRYLEQRGVRHVFGLCGHTNIAVLSAMAGSRVEFI
                     NVRHEQVAAHAADGYARVTRKASVLLTHLGPGLTNAATGVANAALDCIPMVVIAGDVP
                     SHYFGKHPHQEVNLHADGAQYEIYRPFVKRAWRVDDPKLFPEIIDKAFLLAESGQPGP
                     VLVDVPMDVFSADVDSSLFTRLETNTRALRKPSLDLATAREIVSRMAEAKQPLLYVGG
                     GIVLADACDELRAFVDHLGIPVAHSLMGKGALPDDHPLLLGMSGFWGTKLVNDTCLAA
                     DWVLGLGTRFKEADCSSWYRDYTFNIPPTRLIHIDIEPSEIGRNFSTEIGAVADLKQA
                     LQVLTQVAREMFPAPRKAAKMADTIAKFRREFIASNQHMATSDAFPLMPERILAEVRA
                     QLPRDAIITTDVGWNKNGVGQQFPVYTPGSVLTPGGFATMGFGPPAAIGAKIAAPDRV
                     VLSLVGDGGFGQNPSVLATAVEHGLAIIWLVMNNNAYGTIAGLQKAAYGLTHGTLFPA
                     AKVGWDAQKPNYAEIARAYGCEGERLKSAADLGPALSRALKSGKPYLLDVPMKNNPTP
                     TTGHWNILDIYAPDAKLEHAATD"
     gene            8064..8987
                     /locus_tag="VEW08_17320"
     CDS             8064..8987
                     /locus_tag="VEW08_17320"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HYJ42555.1"
                     /translation="MKRDILVSEVGPRDGLQSIKSIMPLAAKKAWISALAASGLREIE
                     VGSFVPAQVLPQLADTAEVVAHALTIPNLTVAVLVPNLRGAEAAVRAGAHKLTIPFSA
                     SETHSLKNVRRTHAQMFDEVRGIVALLAAQPAKTRPKLEGGLSTAFGCTYEGVVPADK
                     VVALAEQLVSAGCDEVGLADSVGFANPAQVRDLVVRVRAVTGKQRFAGLHLHNTRGLG
                     LANVVAGLEVEVDTFDSSMGGIGGCPFAPGATGNIVTEDLVFMLEAMGLHTGIDFEKL
                     MAARTILAAAVPGEELYGYVPAAGLPRGAVV"
     gene            8984..9811
                     /locus_tag="VEW08_17325"
     CDS             8984..9811
                     /locus_tag="VEW08_17325"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HYJ42556.1"
                     /translation="MNRPFSLAHLTMLNVPPPQLVETAARAGYRFVSLRPIPVGAPNE
                     PLYPLGTDRALLARTKAAMAATGVGLLDIEVARIIKEAKPRDYLPALAAAAELGGRHV
                     LSSAWCDDRPYILEFFCELCDLAKPFGLTVDLEFVTWSGIRTLDEAVEVVKDANRSNA
                     GVVVDTLHFDRCHAELEKLSSLPREWFHFAQISDAPASYSTEREELIRIGRAERLYLG
                     EGGIDVGSILGRLPEVPLSIEIPNVAKLESLGAEQYARLCIETARSYLAAGAFRRAG"
     gene            complement(9808..10473)
                     /locus_tag="VEW08_17330"
     CDS             complement(9808..10473)
                     /locus_tag="VEW08_17330"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HYJ42557.1"
                     /translation="MASRKKKAPATTGGDAFANDAERSKADILAVATEEFATYGLSGA
                     RVDAIAKRTRTSKRMIYYYFKGKEGLYRAVLEKAYSEIRSIDSQVGFEDAEPVEAIRK
                     IVEITFDYDETHPYFISLVAVENVHRGRNIAKLPSIKQSGATIIRVLTSILERGAKQG
                     VFRSGVNAIDLHLFISAFCVFRVSNRFTFGKIFDCDLSDPVVRARHKKIFTEAVLRYL
                     EPR"
     gene            complement(10509..>10804)
                     /locus_tag="VEW08_17335"
     CDS             complement(10509..>10804)
                     /locus_tag="VEW08_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42558.1"
                     /translation="MESLPIPENYYYDLGARFGLEDAVLSRMAEFNILYDEDEHGTYH
                     HLYTRAFAKRFFFEVVQRSGYDGYGARDTAIRLAAQSRFRNLAPGKDLPPLHS"
BASE COUNT         1949 a         3488 c         3368 g         1999 t
ORIGIN      
        1 atgttgatac agcgaccgct caacgtgcgg cacagatcgt tcgacaggat ctgcatgtac
       61 cgggcgagca ccaggaggtc gaccttgtag cgcgaaatca gctccagaat ctgcgcttcc
      121 tgttcggccc gcgtcccggc ggtgaccggc agatgatgga atggaatctg gtgccattcg
      181 accaggctgc gcagatccgg gtgattggag atgacgccga ctacgtccac cggaagcacg
      241 cccgagcgcc agcggaacaa cagatcctgg aggcagtggt ccagcttcga cacggcgatc
      301 agcacgcgca cgcgatgcac ggtgtcctcg atctgccagg tcatctcgta ggcgctcgcc
      361 agcgtggcaa acttcgcgcg caggtcttcg atggcaatga acttcgggcc caccgccgtg
      421 aataccgcgc gcatgtagaa gcggcgcgtc agctggtcgt tgaactgttt ggcctcgact
      481 atggaggcgt cgtgatccga caggaagttg gcgaccgcgg ccacgatgcc cttgcgatcc
      541 gggcaaacca gcaacaacga aaaggtggct tcaacctgag acatgcacac tccgctgacc
      601 agcagcggag tgtaccgatt ccggcgtgat tatttgacgc tgtatccccg ggcttcgaga
      661 catgcgcctt ccgcgcggcg atagtctgca cgcttcgagg tcatgtcggt ttcggccacg
      721 ttgccggccg gccgcgtggg atcgaaaccg gtcctttcca ctgcccagcg atggcactca
      781 taacggtcgt tagcctgttg ttcctcgccc tggccgttct tcggataaac gaagacttcc
      841 ccggtggccg gctcggaacc cccctcgggc tcgccacgcg gcggctcggt gaccacgtac
      901 tcacggcgct cgggccgata cgagtagtag gtgttgtcgg cgtagtagta aggcgagccg
      961 ccgtaccaca gcgtggtgta gaacggcggc agcacgccga tgccgattcc aaacggcggc
     1021 gcgacgacca cgtacctcgg gccatagggg cgataccacg agccgccgtg gtagtagtaa
     1081 ggcgagccgc gatagtgggc ggtgtgatag ccgcgcggca gcgcgtaggt tgcgtatccg
     1141 cgcgccgggt agttgtatct gtagttagag taagagccgt ggtgcgaata gctgtagcga
     1201 ttgtagccgg ggtttccgta tccgtaaccg cgtgggccat gaccccgcga cccgtcggcg
     1261 agcgcctcgg acgcgaccac cgcgagcagc agcacgaaca cgactgtcag cattttgtgt
     1321 gtggagcggt gcatgcgaat ctccaaaaac tatttgacag tgtacccgcg tccttccagg
     1381 caggcggtga tggcgcgccg gtaatcgtag gcctgcttct cgaggcgcgc ttgttcggct
     1441 tgttcagccg ccgtttgccg cgatgcgagg cccgcggcct cacgggcacg tgccgaatcc
     1501 gtggccgcgc cgatgaccgc ccccgcaacg gcgccgacca ccgcgccttc ggccgaatcg
     1561 tggggcccgg aaaccgcagc gccgatgacc gcgccggcga cagcaccgtt gaccgcgccc
     1621 cggtggtcag gcggcacggc gaccacctgc aatttctgat gggacgacag cggctgcagg
     1681 ctgggatcgt agccagtctg cttgcctgcc cagccatggc attcgtagcg gtcgcgatcg
     1741 agctggcgtt cgctctgccc attagtggga tacacgtata tatccgcgcc ccttggcgca
     1801 ttggcgtcgg gcgccgacgg agcagtggcg tcgtcggcct gcgctggcgc ggctatcgct
     1861 attgctattg ccatcgccat ggccgcgaac atagccatgg tcatgacggc ctggaaatgt
     1921 tttccgcgca tcgaggtctc ccgcacggtt tcaatggcct ccgtgtctgg gatttcagac
     1981 cggaaatcgc cgtgcaagtt tcacttcagc tcggcgcgat gccgcgttcg ccatagccgg
     2041 aaacagcatc aacaatgccc attggcatat gggaaattgc agcgtcatgt ttgcggcttc
     2101 ggtaacttca attactatgc acgtactggt tcgttaacac aatctcaccg ggaaactgca
     2161 ctgttgatgc gcatacctca ggaagtctca tcgacgatgg atgatcggct gaccgcccgc
     2221 cgtacgcgcg cgcgcttttt cattctcgcg ctgctgaccg tcggcacgct gatcaactat
     2281 ctcgaccgca ccgtgatcag cgtggccgcg ccgctcatga ccaaagaact gggtttgagc
     2341 gccgtggcga tgggggtggt gttttcagcc ttctcgtgga cgtacgcggc ggcgcagatt
     2401 ccgggcggaa tcctgctgga tcgcatcggt gtgcgcctca cctacttcct gtcggtgacg
     2461 atctggtcgg cattcacgct gctccagggc ctcacgatca acctctggac tctcatcgcc
     2521 tgccgcatgg gcctgggcgt ggccgaggcg ccagcctttc cttcgaacag tcgggtcctc
     2581 ggcacgtggt tcccgcaatc tgagcgtgcc tccgcaaccg gtgtctattc gatcggccag
     2641 tacttcggcc tcgctttcct gagccccctg ctcttctgga tcgccggaac cttcggttgg
     2701 cgcgcgctgc tcatcattgt cggcgcggtc ggtattcttt tcgggttcgt ctggtatgcg
     2761 ttctatcgcg atccgcacga aagccgcgcc aaccaggccg agcttgacta catctctgcc
     2821 ggtggcggcg tccccagttc ttccggcgta cgattcgaat ggcgccacat cgctttcctg
     2881 atgcgccagc ggcagattct cggtgcctcg atcggacagt tcgcgagcaa ttccacgctc
     2941 gtgttcttcc tgacctggtt ccccacctac cttgcgaccg agcgccagat gggctggatc
     3001 aaggttggct tcttcacggt gctgcccttc atcgccgcct gcgtcggcgt catgacgggc
     3061 gggctgtgct cggatctgct cctgcgtcgc accggctcgg cgaatgtcgc tcgcaaattg
     3121 ccgatcatcg ccggcctgct gctcgcatca acaatcgtga tcgcgaattt cgtgacgcgg
     3181 gatacactgg tcatcatcgt catgtcggtg gctttcttcg gccagggcat gtgcaacctc
     3241 ggctggactt tgctgacgga cgtggcaccg aaagagctca tgggtctgag tgcgggtgtg
     3301 ttcaatttgt gcgccaacct cgccggcatc gtgacgcccc tggtggtggg cgtcgtggtc
     3361 ggtgtcaccg gctcgttcgc ctgggcgctc gccttcatcg gcatcgtcgc tttgctgggc
     3421 gtcttttcat acgtgttcat cgtcggcgac gtccgccgcg tcgagtacca cgcgtgagcg
     3481 aacatcgcac attgaaagtg ggcctcatcg gcgcggggat ccagcattcc tcgagcccgt
     3541 ccatgcacgt cgacgaaggc aaggcactgg gcctttcgat cacctacgag ctgctcgact
     3601 tcgatctcat gcagggcggg cccaatcggc tggcagcact gctcgacgat gcggagcaac
     3661 gcggcctccg cgggctcaac atcacctatc cgtcgaagca ggctgtcatc ccgctgctgc
     3721 acgaacttgc ccccgaggcc cgcgcgcttc actccgtgaa caccgtgttg ttccgcaatg
     3781 gcaaacgcat cgggcacaac acggactggt ggggtttcgc cgaaagcttc aagcgcgaca
     3841 tgtccgacgt gaacacggac cacgtggtgc tggtcggagc cggcggcgcc ggtgcggcgg
     3901 tgggctacgc catgctgaag cacggctcga aatcactgac gatccatgac atggacggca
     3961 cgcgcgccgc ggcgctggtc gaccgaatgt ccgaactgtt tcccgatcgc agcatcgacg
     4021 tgtgccgcga tcttcccgca tcgcttgcca gggccagcgg cctgatacac gcgacaccga
     4081 cgggcatgcg caaacatccc ggcattccgg tgcccgcgga atccctgcgc cctccgctgt
     4141 gggtatcgga aatcgtctac gtgccgctgt tgacagaact ggtggtcacg gcgcgcaaac
     4201 acggctgccg gacgctcgat ggcggcggca tggccgtgtt ccagggcgcg atggcgttca
     4261 agctgttctt cgacgtggag cctgatatcg cgcgcatgct gcgacggttt cattcccaac
     4321 tgtcgacggt cgcggcgtga ctcttccgat gcgcatcctc gtcctccacg gaatcaatct
     4381 gaacatgttc ggcaagcgcg aggccagcgt gtatggcacc acgacgctcg ccgagatcga
     4441 cgacgcattg aaagttctcg ccagggagct cggcgccgag ctcgaatttt ttcagaccaa
     4501 ccacgaaggc gagatctgcg aacgcattca ccgggtggtc atcgagaaga acatgggcgc
     4561 ggtgatgatc aacgccggcg cctggacgca ctacagctac ggcctgcgcg acgcgctcgc
     4621 catgctcacg gcgcccatcg tcgaagtgca tatgtccaac atccacgcgc gcgaagcgtt
     4681 ccgcacgcac tcggtcatcg ctcctatcgc gaagggccag gtctgcggtt tcggtgtcga
     4741 gagctacctg ctcgggctgc gcgccgccgt atcgctggca aggcaaaaac ccgcgaattg
     4801 accgcctgat tcgatatgta ccaaacggtt cacatcgctt agaatgtacg aaccggtggg
     4861 tggcatcgat tccgagggcg tcgtgagaca actgcaatct gatcctgatc acgctgcgct
     4921 cgacgcgatg atcctgcgtg cgcgcgccgc catgagcgag gtggccgggt acgatcaggc
     4981 gcgcatcgac cgcctgtgcc aggcgcttgg ctgggcgacg gccaatgaaa agactttcaa
     5041 ccgcatcgtc gccatgagca tcgaggagag ccgcctcggt gatcccgagg gacgtaaatc
     5101 caagcggatg aagattctcg gggtgttgcg cgatgccctg cggcaaaaga gcaccggaat
     5161 catcgagcag attcccgagc gcggcatcgt gaagtacgcg aagccggccg gagtcatcgc
     5221 gtcgctggta cccacgacga acccggaact cacgccgccc tacaccggca tctacgcgct
     5281 gaagtgcctg gacgcggtga tcttctcgcc gcatccgcgc tcgaagcgga ccaccttcga
     5341 aatggtgcgc gtgatgcgtg aggcgattcg ccgccagggc gctcccgcgg acctgttcca
     5401 gtgcatcgag aatccgagcg taccgctcac gcagtacctc atggccaatt gcgatctcac
     5461 gctcgccacc ggcggacagc caatggtgcg cgcggcctac agctccgggc gtcccgccta
     5521 tggcgtcggc gccggcaact cgacgatggt gatcgatgag accgccgaca tcgacatcgc
     5581 cgcgcgcaac acccgcatgt cgaagacttc ggacttcggg tctggatgtt cggccgacgg
     5641 caacatcctc atcgactcga ccatctacca cgcattcgtc gccgcgctcg ttcgggaagg
     5701 tggattcctg gccagcgagg aacaagcggc gcagatcgag aaggtgatgt gggacgacca
     5761 tgggcaccgg ctgccggaga ccgtcgcctg ccctcctcag gccatcacgc gcgcggcggg
     5821 gttcgagatt tcggcggatc ggcaattcat catcgtcgaa aatgaccgca tcggccccag
     5881 ggccaactac tcgcgcgaga agctgacgac gctgctcgcc gtgtaccgct acgagggctt
     5941 cgacgaggcg ctcaggatgg tgcggaaaat ctacgaggtg ggcgggaagg gacactcctg
     6001 cggcatctat tcgcacgacg atgatcacat cgaccggctc gcgagagtgg cgcccgtcag
     6061 tcgcatgatg gttcgccagc cgcagtcgaa ggccaatgcc ggctcgttca ccaatggcat
     6121 gcccatgacc tcgagcctgg gatgcggcat ctggggcggc aacatcacca acgagaacat
     6181 ccacctcaag catttcatgc agaccacctg ggtgagccgc cccattcccg aggaccgtcc
     6241 ctccgatcag gagttgtttg gcgagttctt caacaccgag accctgtgat gaaagacacg
     6301 gtcgcaaatc acctggtgcg ctacctggag caacgcggcg tacgccacgt gttcggcctg
     6361 tgcgggcaca ccaacatcgc cgtgttgtcc gccatggcgg gaagccgcgt cgagttcatc
     6421 aacgtgcgcc acgagcaggt ggccgcgcat gccgccgacg gctacgcacg cgtcacgcgc
     6481 aaggcgtcgg tgctgttgac gcacctgggt cccggactca cgaacgccgc caccggcgtg
     6541 gccaatgcgg cgctcgattg cattccgatg gtggtgatcg ccggcgatgt gccctctcac
     6601 tacttcggca aacacccgca ccaggaagtg aacctgcacg ccgacggcgc gcagtacgaa
     6661 atctatcggc cgttcgtgaa gcgcgcctgg cgcgtcgacg atccgaaact tttcccggaa
     6721 atcatcgaca aggcattcct gctggccgag agcggccagc ccggcccggt gctggtcgac
     6781 gttccgatgg acgtattttc cgcagacgtg gattcgagcc tgttcacgcg gctcgagacc
     6841 aatacgcgcg cgttacgcaa gccgtcgctc gacctggcca ccgcgcgcga aatcgtctcg
     6901 agaatggcgg aagcgaaaca gccgctgctg tacgtcggcg gcggcatcgt gctggcggat
     6961 gcctgcgacg aactgcgggc gtttgtcgat cacctcggca ttcccgtggc gcacagcctc
     7021 atgggcaagg gcgcactgcc cgacgatcac ccgttgttgc tgggcatgag cgggttctgg
     7081 ggcacgaagc tcgtgaacga cacctgcctc gccgccgatt gggtgttagg gctcggaacg
     7141 cgattcaagg aggccgactg cagctcctgg taccgcgact acacgttcaa cattccaccg
     7201 acgcgcctca tccacatcga catcgagccg tccgaaatcg gaaggaattt ttctaccgag
     7261 atcggcgcgg tcgccgacct caaacaggcc ctgcaggttc tcacccaggt ggcacgcgag
     7321 atgtttccgg cgcctcgcaa ggcggcaaag atggccgaca ccatcgccaa gttccgccgt
     7381 gagttcatcg ccagcaacca gcacatggcc acctcggacg ccttcccgtt gatgcccgag
     7441 cgcatcctgg ccgaagtgcg tgcgcaattg ccgcgtgacg cgatcatcac cacggacgtg
     7501 ggctggaaca agaacggcgt cggccagcag ttcccggtgt atacgccggg cagcgtgctg
     7561 actccgggag ggttcgcaac gatgggcttc ggcccgcccg cggccatcgg cgcaaagatc
     7621 gcagcaccgg atcgggtcgt gctttcgctg gtcggcgacg gcggattcgg ccagaaccca
     7681 tcggtgctcg ccaccgcggt cgaacacggt ctcgccatca tctggctggt gatgaacaac
     7741 aacgcctacg gaaccatcgc cgggctgcag aaagccgcat atggcctcac gcacggcacg
     7801 ctcttcccgg ctgccaaggt cggctgggat gcgcagaaac ccaactatgc ggagatcgcc
     7861 cgcgcctacg gctgcgaggg tgagcggctg aaatcggccg ccgacctcgg acccgccctg
     7921 tcgcgcgccc tgaaatccgg caagccctat ctactcgacg tgcccatgaa gaacaacccg
     7981 acgccgacca ccgggcactg gaacattctc gacatctacg cacccgacgc gaagctcgag
     8041 cacgcggcga cggattaggt ttgatgaaac gcgacatcct cgtcagcgaa gtggggccgc
     8101 gcgacggcct gcagagcatc aagagcatca tgccgctcgc ggcaaaaaaa gcgtggatct
     8161 ccgcgctcgc ggccagcggc ctgcgcgaaa tcgaagtcgg cagtttcgtg cccgcgcaag
     8221 tgcttccgca actggcggac accgccgagg tcgtcgctca cgcgctcacg attcccaatc
     8281 tgaccgtcgc cgtgctggtg ccgaacctgc gcggcgccga ggccgccgtg cgtgccggcg
     8341 cgcacaagct cacgattccg ttctcggcgt cggaaactca tagcctcaag aacgtgcggc
     8401 gcacccacgc gcagatgttc gacgaagttc gtggcatcgt ggcgttgctc gcggcgcaac
     8461 ccgcgaagac gcggccgaag ctcgagggcg gtctgtccac cgcgttcggt tgcacctacg
     8521 aaggtgtcgt tccggccgac aaggtcgtcg cactcgcgga gcaactggtt tcagccggct
     8581 gcgacgaagt cggtctcgcc gattcggtgg gctttgccaa cccggcacag gtccgcgacc
     8641 tggtcgtgcg cgttcgcgcg gtcacgggca agcagcgatt tgccgggctt cacctgcaca
     8701 acacgcgagg gcttggcctt gccaacgtcg tcgccggact tgaagtggaa gtcgacacct
     8761 tcgacagctc catgggggga atcggcggct gtccgttcgc gcctggcgcg accggcaaca
     8821 tcgtcaccga agatctcgtt ttcatgctcg aggcgatggg ccttcacacc ggcatcgatt
     8881 tcgaaaagct gatggccgca cgcaccatcc tggcggcggc cgtaccaggc gaagaactgt
     8941 atggatacgt accagcggca gggctgccgc gtggagcagt ggtatgaacc ggccattctc
     9001 tctcgcgcac ctgacgatgc tgaatgtgcc gcccccgcag ctcgtcgaaa ccgcggcgcg
     9061 cgcgggctac cggttcgtga gtctgcggcc gattcccgtg ggcgcaccca acgagccgct
     9121 ctaccctctc ggcaccgatc gcgcgctgct cgcgcgcacc aaggcggcca tggcggccac
     9181 cggcgtaggc ctgctcgaca tcgaagtggc ccgcatcatc aaggaagcga agccgcgcga
     9241 ctatctacca gcgcttgcgg ccgccgccga gctcggcggg cgccacgtcc tcagcagcgc
     9301 ctggtgcgac gaccggccgt acatcctcga attcttctgc gagctctgtg atctcgccaa
     9361 acccttcggg ctcacggtgg acctcgagtt cgtgacctgg tccggcatac gcacgctcga
     9421 tgaggcggtc gaagtcgtga aagacgccaa tcgctccaac gccggcgtcg tcgtcgacac
     9481 gctgcatttc gaccgttgcc acgcggagct cgagaagctg agctcgctgc cgcgcgagtg
     9541 gtttcacttc gcgcagatca gcgacgcgcc ggccagctat tccaccgagc gcgaagaact
     9601 gatccgcatc ggccgcgccg agcgtctgta tctcggcgaa ggcggcatcg acgtgggatc
     9661 catcctggga cggctgccgg aggttccact gtcgatcgag atccccaacg tcgccaagct
     9721 cgagtcgctg ggcgcggagc aatacgcgcg gctgtgcatc gaaaccgccc gcagctatct
     9781 tgcggccggc gccttccgcc gggcgggtta acgaggttcc aggtagcgaa gcaccgcttc
     9841 ggtgaagatc ttcttgtgac gcgcgcgaac caccgggtcc gacagatcgc agtcaaagat
     9901 cttgccgaac gtgaaccggt tggaaacccg gaacacgcag aaggcgctga tgaacaggtg
     9961 caggtcgatc gcattcacgc cgctgcggaa cacgccctgc tttgcgccgc gctcgaggat
    10021 ggaggtgagc acacgaatga tcgtggcgcc gctctgcttg atggacggca gcttggcgat
    10081 gttgcgcccg cgatgcacgt tctcgacggc caccaggctg atgaaatagg gatgggtttc
    10141 gtcgtagtcg aaggtgattt cgacgatctt gcggatggct tcgaccggtt cggcatcctc
    10201 gaaacccacc tggctgtcga tggagcggat ttccgagtac gccttctcga gcacggcgcg
    10261 ataaaggcct tccttgccct tgaagtagta gtagatcatc cgcttcgaag tgcgcgtgcg
    10321 ctttgcgatg gcgtcgacac gcgcgccact cagaccatag gtggcgaact cttcagtcgc
    10381 tacggccagg atgtcggcct tggaacgttc ggcatcattg gcaaatgcat cgcctccggt
    10441 cgttgccggg gctttcttct tacggctcgc catccacgaa tccctccatt tcgcccgcga
    10501 cagtacactc aagaatgcag cggcggaagg tccttgcccg gcgcaaggtt ccggaatcgc
    10561 gattgggccg ccaggcggat ggccgtgtcc cgggcgccgt acccgtcgta cccggatcgc
    10621 tgcacgactt cgaagaaaaa tcgcttggcg aacgcgcgcg tgtagagatg gtggtatgtg
    10681 ccgtgctcgt cctcgtcgta caggatgttg aactccgcca tgcgcgataa caccgcatcc
    10741 tcgaggccga accgcgcgcc aaggtcgtag taatagttct cggggatagg cagcgattcc
    10801 atgc
//
LOCUS       DASQJS010000439         4324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2486723, whole genome shotgun sequence.
ACCESSION   DASQJS010000439 DASQJS010000000
VERSION     DASQJS010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486723"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VEW08_17340"
     CDS             complement(<1..1077)
                     /locus_tag="VEW08_17340"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HYJ42559.1"
                     /translation="MRRDDIQFPAKHSALRDDVHVLGALIGDVLKEQGGDTLFDLVEK
                     DRRLSIRRRAGDKEAAAELSVQLRGRAPQVARDLARAFSMWFRSVNLAEKVHRIRRRR
                     GYFIEASERAQPGGVEAALTELKNRNFSLVEVLELLKKIRIEPVFTAHPTESARRTML
                     RKNQRIAELLLDRLDPTLTPQELRQNWSRVRTELTTAWQTEDHPRERLTVADEREHVV
                     FYLAEILYRILPAFYDELAEALARLYGVHPDSLELPNMIRFGTWVGGDMDGNPDVHAK
                     TIRETLARQQQVIINAYFKECGVLAQLLSQSGSRSAVNPELSQRIELYATLIPGALGI
                     TPMRHDRMPYRVFLGQVSERLRLTY"
     gene            1143..2057
                     /locus_tag="VEW08_17345"
     CDS             1143..2057
                     /locus_tag="VEW08_17345"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYJ42560.1"
                     /translation="MDQEKGRQRLGMLFVAGAAILFASKGLFAKALYQRGVGFELLVT
                     VRAVLAVPLFAWFAVRSNPTPAPMRTRAVLAAVIAGITCYYVGALVDFWALTLIDASI
                     ERVLLFSFPAIVVIIGSVLRRRAPEASVVAALVVTYIGIFFAMGGIDLSELRQNLFGA
                     SLVLIAALTTAIYFLIGERYTNELGSTRFAAIGMSASATMLALHFVAFRSVDELRTLQ
                     AYDWMLLVILGVFCVFIPGLLQAEGMRRVGAERGAIGSTIGPPTTIFLAALFLDERLT
                     GWQMVGSAMIIGSVLVLSWPKRVVADEP"
     gene            2081..2542
                     /locus_tag="VEW08_17350"
     CDS             2081..2542
                     /locus_tag="VEW08_17350"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HYJ42561.1"
                     /translation="MTPARSKSRFTRFAKATAHASGQPSAFMLAVLVVAAWVISGPLF
                     HWSDTWQLVINTGTTIVTFLMVFLIQSTQNRDAIAVQVKLDELLRVTPGAHNVLMNLE
                     ELEEHELERIRAVYVALAEKAREGVDDGRSDEGVPEIGELIEKSKPGGKAR"
     gene            complement(2547..2990)
                     /locus_tag="VEW08_17355"
     CDS             complement(2547..2990)
                     /locus_tag="VEW08_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42562.1"
                     /translation="MIRAILCYVALALLSAGSFASPPKPAESEYFVTREADIFIVGGK
                     GLCYTLSIELRKPLAAPIYITVDFEDPADRDHPFFVEREVMPGEHVVQVLSEPFRAIK
                     NGGKYLVKVWIYEDALRRKPLGIHEQMVQFKVPGVVFNTFGIEKL"
     gene            complement(2999..>4324)
                     /locus_tag="VEW08_17360"
     CDS             complement(2999..>4324)
                     /locus_tag="VEW08_17360"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYJ42563.1"
                     /translation="ELFTLAVLLVALASAWASQMAGLSMALGAFLAGMMLAETEYSHQ
                     VESVIRPFRDILLGLFFISVGMLLDFGALWREIWVILALLVSMVLIKAVIASFATRLF
                     VDSNFKAVRTGVTLAVGGEFGIALLTLLLQNEAIDPAIAQPLLVTVVLGMVLAPVILA
                     NNKRVARLLLGERGPPKTATEREDAATQAVAKREHVILCGFGRVGQNVARVLEAQNFE
                     FIAIDLDAVRVRAARQIGQPVVYGDSADEQVLEEVGLANASAVVISFSDTRTSLAILR
                     SLRRLRSDVPVLVRTPDDSRLLELKAAGATEIIPEAFEASLMLASHVLMSLKVPPGVV
                     MRTVAELRGNRYEMLRNVIARGDGMSNDAEFAEQMRSVVLPPGSWCIGKCVDEVRGAG
                     AEVAFTGIRRQGILGREPTGDTEFQDGDIVVIYGTPEALEHAEAVLLAG"
BASE COUNT          756 a         1377 c         1403 g          788 t
ORIGIN      
        1 gtaggtgagg cgcagccgtt ccgacacctg ccccaggaac acgcggtacg gcatgcggtc
       61 gtggcgcatc ggcgtgatgc cgagcgcgcc gggaatcaga gtggcgtaca actcgatgcg
      121 ctggctgagc tccggattca ccgcgctgcg actgccgctc tgcgagagca gttgcgccag
      181 cacgccgcac tccttgaaat acgcgttgat gatcacctgc tgctgacgcg cgagagtctc
      241 gcggatggtc ttggcgtgca cgtccgggtt gccgtccatg tcgccgccga cccaggtccc
      301 gaagcggatc atgttcggca actcgagcga atccggatgc acgccgtaga gcctggcgag
      361 cgcctcggcg agttcgtcgt aaaacgcggg caggatccgg tacaggattt cagcgaggta
      421 gaacactacg tgctcgcgct cgtcggccac cgtcaggcgt tcacgggggt gatcttcggt
      481 ctgccacgcg gtggtgagtt cggtgcgtac gcggctccag ttctgccgca gttcctgcgg
      541 ggtcaacgtc gggtccagcc gatccagcag cagttcggcg atgcgctggt tcttgcgcag
      601 catggtgcgc cgggcgctct cggtgggatg cgcggtgaac accggttcga tgcggatctt
      661 cttcagcagc tccagcacct cgacaagcga gaagttgcgg ttcttgagct cggtgagcgc
      721 tgcctcgact ccgcccggct gggcgcgttc gctggcttcg atgaagtagc cgcggcggcg
      781 gcggatgcga tgcactttct cggcgagatt gaccgagcgg aaccacatgg agaaggcacg
      841 cgccaggtcg cgggccactt gtggcgcgcg cccgcgcaac tgtactgaca gctcggccgc
      901 cgcttccttg tcgccggcgc gccggcggat agatagacgg cggtccttct ccaccaggtc
      961 gaacagggtg tctccgccct gctccttcag cacatcgccg atgagcgcgc cgagcacatg
     1021 cacgtcgtcg cgcagcgcgg aatgtttggc cgggaactgg atgtcgtcac gtctcacgga
     1081 gcgcgcgatt ctacagtgtt aaagagatgg cgtagcgccg ccttgtcggg aatcatgcac
     1141 ccatggatca ggaaaagggg cgccagcgcc tcggcatgtt gttcgtggcc ggtgcggcca
     1201 tcttgttcgc cagcaagggc ctgttcgcca aggcccttta ccagcgaggc gtgggcttcg
     1261 agctcctggt gacggttcgc gccgtgcttg cggtgccgct gttcgcctgg ttcgccgtgc
     1321 gttccaaccc cacgcccgcg ccgatgcgaa cccgcgcggt tctcgcggcg gtcatcgccg
     1381 gcatcacctg ctactacgtc ggcgcgctgg tggatttctg ggcactgacg ctcatcgacg
     1441 cgtcgatcga gcgcgtgctg ctgttcagct tcccggcgat cgtggtgatc atcgggtcgg
     1501 tgctcaggcg gcgcgccccg gaggcatccg tggtcgcggc cctggtcgtc acctacatcg
     1561 gtatcttctt cgccatgggc ggcatcgacc tctcggaact gcggcagaac ctgttcgggg
     1621 cctcgctcgt gctcatcgcc gcgctcacca ccgctatcta cttcctcatt ggcgaacgct
     1681 atacgaatga gctcggcagc acgcgcttcg ccgccatcgg catgagtgcg tcggcgacga
     1741 tgctggcgct gcacttcgtc gcgtttcgct ccgttgacga gctgcgcacg ttgcaggcct
     1801 acgactggat gctgctcgtc atcctgggtg tcttttgcgt gttcatcccc ggactgctgc
     1861 aggccgaagg aatgcgacgc gtcggggcgg agcgcggcgc cataggcagc accatcggcc
     1921 cgcccaccac cattttcctg gcggcgctgt tcctcgatga acgcctgacc ggctggcaga
     1981 tggtcggcag tgcgatgatc atcggcagcg tgctggtgct gagctggccg aaacgcgtag
     2041 tcgctgacga gccctgaaaa attctcccga ggagtgggtc atgacccctg caagatcgaa
     2101 atcccggttc actcgtttcg ccaaggcgac tgcgcacgcc agcggccaac cgtcggcgtt
     2161 catgcttgcg gttctcgtgg tagccgcatg ggtgatcagc ggaccgctct ttcactggag
     2221 cgacacctgg cagctcgtga tcaacacggg caccaccatc gtcacatttc tgatggtgtt
     2281 cctgatccag agcacgcaga accgcgatgc gatcgccgtt caggtaaagc tcgatgagct
     2341 gctccgcgtc actccgggcg cccacaatgt gctcatgaat ctggaagaac tcgaggagca
     2401 cgagctcgag cgcattcgcg ccgtgtacgt cgcgcttgcg gagaaggcgc gcgagggcgt
     2461 ggatgacggc cgcagtgatg aaggcgtgcc ggaaatcggc gaactgatcg aaaagtcgaa
     2521 gcccgggggg aaagcgcgct gaagcctcag agcttctcga tgccgaaggt gttgaatacc
     2581 acgcccggca ctttgaactg caccatctgt tcgtggatgc cgagcggctt cctgcgcagc
     2641 gcgtcctcgt agatccacac tttcacgaga tacttgccgc cattcttgat ggcacggaaa
     2701 ggttccgaga ggacttgcac gacgtgctcg cccggcatca cttcccgctc gacgaagaat
     2761 ggatggtcac gatccgccgg atcttcgaaa tccacggtga tgtaaatggg cgctgcgagc
     2821 ggtttgcgca actcgatgga tagcgtgtag cacagcccct tgccacccac gataaagata
     2881 tcggcttcgc gcgtgacgaa atactcggat tcggccggct tcggtggcga ggcgaacgag
     2941 cccgcgctca acaaggcgag cgcgacgtaa cagagaattg cgcgaatcat ggcacctcct
     3001 accctgccag cagcactgcc tccgcatgtt ccagcgcctc gggagtgcca tagatgacca
     3061 ctatgtctcc gtcctggaat tccgtatctc ccgtgggttc acgaccgagg attccctggc
     3121 gccggatgcc ggtgaaggca acttccgcgc cggcgccgcg aacttcatcc acgcacttgc
     3181 cgatacacca cgagcccggc ggcagcacca ccgagcgcat ctgttcggcg aactccgcat
     3241 cgttggacat gccgtcgccg cgggcgatga cattgcgcag catctcgtag cggttgcctc
     3301 gcagttcggc cacggtacgc atcacgacgc cgggcggtac cttgagcgac atcagcacgt
     3361 gcgaggccag catgaggctg gcctcgaaag cttccgggat gatctcggtg gcgccggccg
     3421 ccttgagttc gagcaggcgc gaatcgtcgg gcgtgcgcac cagcaccggc acgtcgcttc
     3481 gaagtcggcg cagcgagcgc aggatggcga gcgaggtgcg cgtgtcggag aacgagatga
     3541 ccacggcgct ggcgttcgcg aggcccactt cttcgagcac ctgctcatcg gccgaatccc
     3601 cgtacaccac cggctggccg atctgtcgtg cagcacgtac tcgaaccgcg tcgagatcga
     3661 tggcgatgaa ttcgaagttc tgcgcctcga gcacgcgcgc gacgttctgg cccacgcggc
     3721 cgaagccgca gaggatgacg tgctcgcgct tggcgacagc ctgcgtggcg gcgtcttcac
     3781 gctcggtggc agtcttgggt ggcccacgct cacctaacaa cagccgggcg acacgcttgt
     3841 tgttggccag gatgaccggc gccagcacca tgccgagcac cacggtgacc agcagaggct
     3901 gcgcgatggc cggatcgatg gcttcgttct gcaacagcag cgtgagcagt gctatgccga
     3961 attcaccgcc gacggccagc gtgacgccgg tgcgcaccgc cttgaaattg gaatcgacga
     4021 acaggcgcgt ggcgaacgag gcgatgacgg ccttgatgag caccatcgaa accagcaacg
     4081 cgaggatcac ccagatctcg cgccacaggg cgccgaagtc gagcagcatg ccgacggaga
     4141 tgaagaacag gccgagcagg atgtcgcgga agggccggat gacgctctcg acctggtggc
     4201 tgtattcggt ctcggccagc atcatgcccg caaggaatgc gccgagcgcc atcgacaggc
     4261 cggccatttg cgatgcccag gccgaggcca gcgccaccag cagcacggcc agcgtgaaca
     4321 gttc
//
LOCUS       DASQJS010000440         5428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4681028, whole genome shotgun sequence.
ACCESSION   DASQJS010000440 DASQJS010000000
VERSION     DASQJS010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4681028"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2675
                     /locus_tag="VEW08_17365"
     CDS             <1..2675
                     /locus_tag="VEW08_17365"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYJ42564.1"
                     /translation="TLPQGSIGPFFNDDFGDVYGVIYALTSDGFTYRELRDQAEFIRA
                     ELLRVNNVGKVDLIGVQDEVIFIDFSLRQMAGLGIDPDLVALTLASQNIVIASGTIET
                     PLERIVVRVSGALDSVTTLENIAIRVGERRVRLRDFAQVTRGYKDPPTPQYRYNGIPA
                     IGIGVSMSKGGNVLDLGTALEKELTRIESDLPVGIDVHRVANQPEVVAESVGQFTRSL
                     FEAIIIVLIVSFVSLGARAGLVVAISIPLVLAITFVFMEMFGISLQRISLGALIIALG
                     LLVDDAMIAVEMMVKKTEEGWDRFRAATYAYTSTAFPMLTGTLVSVAGFLPVGFAKSG
                     AGEYCFTLFAVVTIALLVSWVVAVVFTPYIGVAVLKEPVPGEHHAHAESAMTARFRRV
                     LLKSLANRGWVVAGTAGAFVIAVILFGFVQQQFFPSSSRPELMIDLRLAQNASIEATM
                     AQVQRFEKVLQADDDIVYHSFYVGSGAVRFYLPLNQQLENANFAQAVVITKSYEVRDQ
                     VRARLEKVLDEEFGTLMARVEPLALGPPVDWPLQYRVSGPDVTGVRAISETVAATLRA
                     NANTRVVNFDWNEMTKQMRIEVDQEKARQLGISSEQVSRALASAVSGRTVTQFRDDIY
                     LIDVQGRAQARDRGDIGRLRDVEIGIASGNSVPLTQIAKFEYGLEETVIWRRNRLPTI
                     TVQSKMAEGLTAATVTEQLQEPIEALMAKLPPGYRIEIGGAVGESAKGSASIMAVVPV
                     MFVLMLFILMLQLHSTQKLLLVMLTAPLAIIGVTLALLISNRPFGFVAMLGVFALVGM
                     IIRNSVVLMAQIKENEDAGMERGAAIVDATMHRLRPILLTAAAAILGLIPIVSEVFWG
                     PMAIALMGGLFIATVLTLIFLPALYASWYRVKRV"
     gene            complement(2681..3952)
                     /locus_tag="VEW08_17370"
     CDS             complement(2681..3952)
                     /locus_tag="VEW08_17370"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYJ42565.1"
                     /translation="MNQRPTLLIVSYHFAPSPLVGAKRFSFLTREFARLGFNVHVITS
                     GKGESPHGRDSSLPVCGTVHRVDAPFEVPLKGGGLIHRIAGALMRRILAPVGMDYFWA
                     RAATRKALEVAAKLPRGVVIATSPPHAALIAGARVARRLKWPLILDYRDPWSAYDWPE
                     WHRGWISQWFARRIEGALVRRSAARVLNTPSMRDWFEESFPHAASAMNFVIPNGFNAG
                     PAPAPLAGGPIEIVHAGEIYGSRSLVPLLRAVERLSQRHRERTIHVTSYGPLPDTEWQ
                     RIRGAGLESFIEERPRIPFAELFAELQRAHVLLAVVSEHMTYSVPYKIYDYMAAGRPI
                     LGLAPRDAALHELLADSDAGHCVEPADIDGIGQALEKILFSAAPPARTRIDHFRWPNL
                     AQDYLNAIDAASGPSRALSESRTHLVTRTDS"
     gene            complement(3949..5382)
                     /locus_tag="VEW08_17375"
     CDS             complement(3949..5382)
                     /locus_tag="VEW08_17375"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HYJ42566.1"
                     /translation="MSSFVSRGAVLASARLLNQALALLSPLLLVRLLEISEYGRYRQF
                     MATAMFITSLAGFALSANLNYLIARSPKRQPVDITNTCMLMLGVSVLSALVVVLARQW
                     IVPPEISSAWLLLAVYVFLFLNLEVLVSYWLAHGKSVSVMGYTLAVTAWRIVTLLAAT
                     YWLNDVQLIFVTIVCAEAVKNLWIYVWLRRRGLLVFRWDAAAMREQIRLVAPLGAGSV
                     LYKLNDFGKVVVANQMGPVPLALYTTAAYQVPLVNIVQGALADVIFPDMVKRSRTDPA
                     QGLLLWKRAQVLIFAIICPAWLLLTYWAEPFVRLLFTDAYVAATPYFQVFLLLMVRQC
                     FQFSTPLRSVEDNASFAHANLVALAINATLIVTLMPKYGLWGPTLGLVIGQTWTSFYL
                     GSRVLKRYQLPLSELCQWGKVALALAASLVALAAMHATQLYVPHGVAGLLASLGAFGL
                     VYAISARIILREEYGYVMRTIVRRKTA"
BASE COUNT          990 a         1654 c         1831 g          953 t
ORIGIN      
        1 gaacgctgcc gcaaggttcc atcggtccat ttttcaacga cgatttcggt gatgtgtacg
       61 gcgtgatcta cgcgctgacc agcgatggct tcacgtatcg ggagctgcgt gaccaggccg
      121 aattcatccg cgccgaactg ctgcgcgtga acaacgttgg caaggtggat ctcatcggcg
      181 tgcaggacga agtcatcttc atcgacttca gcctgcgaca gatggccggg cttggcatcg
      241 atccggacct ggtggccctg acactcgcca gtcagaacat cgtgatcgcc tcgggcacca
      301 tcgagacgcc gctggaacgc atcgtcgtgc gtgtgagcgg cgcgctcgat tcggtgacca
      361 cgctggagaa catcgccatt cgcgtgggcg agcgacgcgt gcgcctcagg gatttcgcgc
      421 aggtgacacg cggctacaag gacccgccca cgccgcaata ccgttacaac ggcatccccg
      481 ccatcggcat cggtgtttcc atgtccaagg gcggcaatgt gctcgacctg ggcacggcgc
      541 tcgagaaaga gctcacgcgc atcgaaagcg atctgccggt gggcatcgac gtgcatcgcg
      601 tggcgaacca gcccgaggtc gtcgcggaat cggtcggcca gttcacgcgc agcctgttcg
      661 aggccatcat catcgtgctc atcgtgagtt tcgtcagcct gggtgcgcgc gccggtctcg
      721 tggtcgcgat cagcattccg ctggtgctcg ccatcacgtt cgtgttcatg gagatgttcg
      781 ggatcagcct gcagaggatt tcgctgggcg cgctgatcat cgcccttggc cttctggtcg
      841 acgatgccat gatcgcggtc gagatgatgg tgaagaagac ggaagagggc tgggaccggt
      901 tccgcgccgc cacgtacgct tacacctcga cggcgttccc catgctcacc ggcacgctgg
      961 tgtcggtggc ggggtttctg ccggtgggat tcgcgaagag tggcgccggc gaatattgct
     1021 tcacattgtt cgccgtggtg accatcgcct tgctcgtttc ctgggtggtg gcggtggtgt
     1081 tcacgccgta catcggcgtc gccgtgctca aggagcccgt gccgggcgaa catcacgcgc
     1141 acgccgaaag tgcaatgact gcgcggtttc gccgcgtgct gctgaaatcc ctggcgaacc
     1201 gcggctgggt ggtggccggc accgcgggcg cgttcgtcat cgccgtgatc ctgttcggct
     1261 tcgtgcagca gcagttcttt ccttcatcga gccggccgga actcatgatc gacctgcgcc
     1321 tggcgcagaa cgcgtcgatc gaggccacca tggcgcaggt gcagcggttc gaaaaggtcc
     1381 tgcaggccga cgacgacatc gtctatcaca gtttctacgt gggctccggt gcggtgcgct
     1441 tctatctacc gctcaaccag cagctcgaga acgccaattt tgcgcaggcg gtggtcatca
     1501 cgaagagcta cgaggtgcgt gaccaggtgc gcgcccggct cgagaaggtg ctcgacgagg
     1561 aattcggcac gctgatggcg cgcgtcgagc cgctggcgct cgggccgccg gtggactggc
     1621 cgctgcagta ccgcgtcagc ggaccggacg tgaccggggt gcgcgcgatt tccgaaaccg
     1681 tggcggccac gctgcgcgca aacgccaata cgcgggtcgt gaatttcgac tggaacgaaa
     1741 tgaccaagca gatgcgcatc gaggtggacc aggagaaggc gcgccagctc ggcatcagct
     1801 ccgagcaggt atcgcgggcg ctggcctcgg cggtctcggg ccgtacggtc acgcagttcc
     1861 gcgacgacat ctatctcatc gacgtgcagg gccgtgcgca ggcacgcgat cgcggcgaca
     1921 tcgggcggtt gcgcgacgtc gagatcggca tcgccagcgg caattcggta ccgctcacgc
     1981 agatcgcgaa attcgagtac ggcctggagg aaaccgtgat ctggcggcgc aaccggctgc
     2041 cgacgatcac cgtgcagtcg aagatggccg aggggctgac tgccgccacc gtcaccgaac
     2101 agttgcagga gcccatcgag gcgctcatgg ccaaactacc gccgggttat cgcatcgaga
     2161 tcggtggtgc ggtgggcgag agcgccaagg gctcggcgtc catcatggcg gtcgtgccgg
     2221 tcatgttcgt cctcatgctg ttcattctca tgctgcagct tcacagcacc cagaagctgt
     2281 tgctggtcat gctcacggcg ccgctggcga tcatcggcgt cacgctcgcg ttgctgatca
     2341 gcaaccggcc gttcggattc gtggccatgc tcggcgtgtt cgcgctggtg ggcatgatca
     2401 tccgcaattc ggtggtgctg atggcgcaga tcaaggagaa cgaggacgcc ggcatggaac
     2461 gcggcgcggc aatcgtcgat gccaccatgc accggttgcg gcccatcctg ttgacggccg
     2521 cggccgccat cctcgggctc attcccatcg tcagcgaagt gttctggggc ccgatggcca
     2581 tcgcgctgat gggcgggctg ttcatcgcca ccgtgctgac gctgatcttc ctgccggcgc
     2641 tgtacgccag ctggtatcgc gtgaagaggg tgtgacgtcg ctacgagtcc gttcgtgtca
     2701 cgagatgcgt tcgggattca gataacgcac gcgaaggccc ggacgcggcg tcgatggcgt
     2761 tcagataatc ctgcgccagg ttcggccagc ggaaatggtc gatacgggta cgcgctggcg
     2821 gggcggcgct gaacaggatc ttctcgagcg cctgcccgat accgtcgatg tccgcgggtt
     2881 ccacgcagtg tcccgcgtcg ctgtcggcga gcagttcgtg cagcgctgca tcacgcggcg
     2941 cgagcccgag gatgggtctg ccggcagcca tgtagtcgta gatcttgtaa ggcacggaat
     3001 aggtcatgtg ctcgctgacc accgccagca gtacgtgggc ccgttgcaat tcggcgaaca
     3061 actcggcgaa cgggatgcgg ggccgctctt cgatgaacga ttcgaggccg gcgccgcgaa
     3121 tgcgctgcca ttcggtgtcg gggagtggac cgtagctggt cacgtggatc gtgcgctccc
     3181 ggtggcgctg gctcaggcgc tccacggcgc gcaacagcgg caccagtgaa cggctgccat
     3241 agatttcgcc ggcatgcacg atttcaatgg gcccgcccgc gagcggcgcg ggcgctggcc
     3301 cggcattgaa gccattggga atcacgaagt tcatcgccga cgcggcgtgc ggaaaacttt
     3361 cctcgaacca gtcgcgcatg ctgggcgtat tcaacactcg tgccgcgctg cgccgcacga
     3421 gggcgccctc gatgcgacgc gcaaaccatt gcgagatcca gccgcggtgc cactccggcc
     3481 agtcgtaggc gctccatgga tcccggtagt cgaggatgag cggccacttc agtcgccgcg
     3541 ccacgcgcgc gccggcgatg agcgccgcgt gcggtggcga agtggcgatg acgacgccgc
     3601 gcggtagttt ggctgcgact tcgagcgcct tgcgcgtggc cgcgcgcgcc cagaagtagt
     3661 ccatgcccac gggcgcgagg atccggcgca tcagtgcgcc ggcgatgcgg tgaatcagtc
     3721 cgccaccctt gagcggcact tcgaacggcg cgtcgacacg gtgcacggta ccgcagacgg
     3781 gcagcgagct gtcgcgtccg tgaggcgatt caccttttcc gctggtgatg acgtggacgt
     3841 tgaacccaag gcgcgcgaat tcgcgcgtga gaaagctgaa gcgcttggcg cccacgagcg
     3901 ggcttggggc gaagtgatag gacacgatga gcaaggtggg gcgttggttc atgcggtttt
     3961 ccgcctgacg attgtgcgca tcacgtagcc gtattcctcg cgcaggatga tgcgcgccga
     4021 aatggcgtac accaggccga aagcgccgag cgaggccagc agcccggcaa cgccgtgcgg
     4081 cacgtacaac tgcgtggcgt gcattgccgc caacgcgacc agggatgctg ccagcgcgag
     4141 tgcgaccttg ccccattggc aaagctcgga cagcggcagt tgatatcgct tgagtacgcg
     4201 cgatcccagg tagaaagatg tccaggtctg cccgatcacg agcccgagcg tcggccccca
     4261 gaggccgtac ttcggcatca gcgtgacgat gagcgtggcg ttgatggcga gcgcgacgag
     4321 attggcatgt gcgaatgaag cgttgtcctc gacgctgcgc agtggcgtcg agaactggaa
     4381 gcactggcgc accatgagca gcaggaaaac ctggaagtag ggcgtggccg ccacgtacgc
     4441 gtcggtgaac agcaggcgca cgaacggctc cgcccagtag gtcagcagca accacgcggg
     4501 gcagatgatg gcgaagatca gtacctgcgc gcgtttccac aacagcagtc cctgcgcggg
     4561 atcggtcctg gatcgcttga ccatgtcggg aaagatcaca tccgccagcg cgccctggac
     4621 gatgttcacc agcggcacct ggtaggccgc ggtcgtgtag agcgcgagtg gcacgggccc
     4681 catctggttg gccaccacca ccttgccgaa gtcgttgagc ttgtagagca ccgagccagc
     4741 gccgagcggc gcgaccaggc ggatctgttc acgcatggcc gcggcatccc agcggaagac
     4801 cagcaggccg cgtcgcctca gccacacgta gatccacagg tttttcactg cctcggcaca
     4861 aacaatagtg acaaagatga gttgtacgtc gttgagccag taggtggcgg ctaacaacgt
     4921 gacgattcgc cacgccgtca cggccagcgt gtagcccatg accgacacgg actttccgtg
     4981 cgccagccag tacgaaacca gtacttcgag attgaggaag aggaaaacgt agacggcgag
     5041 cagcaaccag gccgaggaga tttccggcgg cacgatccac tgccgtgcca ggaccaccac
     5101 gagtgcgctc agcacgctga cgccgagcat gagcatgcag gtgttggtga tgtccacggg
     5161 ttgacgtttg ggagaacgcg caatgaggta gttgaggttc gcgctcagcg cgaaaccggc
     5221 cagcgacgtg atgaacatgg cggtcgccat gaactgccgg taacggccgt actcggagat
     5281 ctcgagcagg cgcaccagca acaagggact cagcaacgcg agggcctgat taagcaggcg
     5341 cgcgctggcg agtacggcac ctctcgagac gaatgaactc atggggtacg ggattttagg
     5401 agcgcggtca aacgcggcac tgtcggac
//
LOCUS       DASQJS010000441        11331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4681172, whole genome shotgun sequence.
ACCESSION   DASQJS010000441 DASQJS010000000
VERSION     DASQJS010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11331
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4681172"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VEW08_17380"
     CDS             <1..521
                     /locus_tag="VEW08_17380"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ42567.1"
                     /translation="SAAPVEDTTLALWQKNMDILATGYFLIARQAFQLFKTQAIGGSI
                     VFIGSKNALAASPGAAAYCTAKAAELHLARCLALEGAPLGIRVNVVNPDAVLRGSRIW
                     QGEWATQRAAQNKTSVDNLETVYRERSLLKRSVYPEDVAEAVAFFASERSAKSTGNIV
                     NVDAGNAGAFTR"
     gene            540..1844
                     /gene="rhaI"
                     /locus_tag="VEW08_17385"
     CDS             540..1844
                     /gene="rhaI"
                     /locus_tag="VEW08_17385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose catabolism isomerase"
                     /protein_id="HYJ42568.1"
                     /translation="MSKAFADPRDASYIASANQPQAAALATDYTALGIQLERRGIKID
                     DITRAVANFKVAVPSWGVGTGGTRFGRFPGPGEPRNVFEKLEDCAVIHKLTGATPTVS
                     LHIPWDRTDDYKGLREHGAALGLGFDAMNSNTFQDQVGQSLSYKYGSLTHVDAAVRAQ
                     AIAHNIDCIAAGKALGSKALTVWIGDGANFPGQSHLGRAFERYLESTRSIYAALPEDW
                     RMFIEHKMYEPAFYATVIQDWGSSLMAAQTLGPKAFCLVDLGHHAPNVNIEMIVARLV
                     HAQRLGGFHFNDSKYGDDDLDAGSIEPFRLFLVFNELVEAELAKAPNFRPAHMLDQSH
                     NVTDPIESLMTSAIELQRAYAQALIVDRKSLHEYRMGNDVLMAAQTLKRGFTTDVTPI
                     LAQARVLKGAAIDPVGTYRQSGYRGKCAEMRPGKQSLGGGIV"
     gene            1909..3054
                     /locus_tag="VEW08_17390"
     CDS             1909..3054
                     /locus_tag="VEW08_17390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF008398.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HYJ42569.1"
                     /translation="MPVLNSIEDLRQEARRRVPRAIFDYADRGSYDEVTFNRNLADLA
                     ALQLRQRVMVDVSQQQLATTILGEEWTIPVGIGPTGLTGLFHANGEMLGARAAQAFGV
                     PFCLSTMSICSIEDVRSAVQKPFWFQVYLMRDRSFNAELLDRALAANCSAIMLTVDMP
                     VQGLRRRDPKNGLSIPPKLTLKNMFEVAMRPTWALRVLTGRRRTFGNLEARMKGTGGL
                     KTLSEWIAQQFDPTVTWKDLAWLRERWPRKIIIKGILDAEDASLACQHGVDAMVVSNH
                     GGRQLDGARSTISALPEVVEAVAGRCDVLFDGGVRCGQDVLRALALGAKGCLIGKAFL
                     YALAARGEAGVTLALDIMRKELAVSLALTGANDVRKVTRDILVAPGA"
     gene            complement(3186..6257)
                     /locus_tag="VEW08_17395"
     CDS             complement(3186..6257)
                     /locus_tag="VEW08_17395"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ42570.1"
                     /translation="MNSKRFAGQARKGRAAVYLLTGLALGSATSALAQDPSTPPAEEG
                     DVIVVTGSRIARDVTDASTPLMIIDADEITLSGATSIDKVLNDQPQFVAATNGGATAN
                     TVPAGSAAGAAYVNLRGFGPTRSLTLVNGRRFAIFGPEQVTDLNTIPTALIERTEVVT
                     GGSSAVYGSDAITGVVNFIMKDDFEGVKIGGQYGFDSSTSTPTFSTDLTIGGNFAEGR
                     GNAVVSLNYYKREGFTRHERGDWAELPYGEGCVTADSWSDSLIGVANGSNAANCATSG
                     GRMGFIFSGSGDIPNGRFVLTPAQLAATQTQLAAAGLAGLGSNGFTFNDAGAQGSQRL
                     VNRPADDFNLTQFNYLQVPQERWMLNAFTHYEFAPKATGYMEFHFSNNRVDQQLTQAN
                     IGGDFLFNTNNPYLDANMRATLVALDAAETGPRTLQQGPIAYTTTPNDGLAILTAGRR
                     LVELPFRHNVDDHNVWRMALGMKGDLGDASDKFLRGLNYDVYFTFSRSEDSSRQEGAA
                     SRSRYAAALLSSGGNPPVANIFGQNLSTAAVNAIKINATNTTNAEQKVAVATLGGEAF
                     DLPAGAVNFSVGLEWRSAEAEYIPDEYLRSGDVIGFNPGLPTAGEVTAKEIFAELRVP
                     ILADLPFVQSLTANGGYRSSDYDLDGVGRVETYLYGLDWRVNESVGFRGQFQRAIRAP
                     NIGDLYGGLQLNFQTLTDPCSSRNTANRTAAVRALCEATGVPAASVFTAGVQPDNIIP
                     TRSGGNVDLHEESSDTTTLGVVLTPTFVDDLVVTLDYFDIKLNGAIAQLGGGAQNTLN
                     LCYFTVQDAGSDFCQAVHRNPASGGITVPYSLDVLQANIGRLATIGYDLGARYGWDVG
                     FGMEGGSRFDIGTQWTYTDKFTITPMQAVPANKNRCVGAYGSTCGEPIPQYKGVTRFT
                     WTTGPLGVSLRHRFIDSVTTDRIVLPKTSHVGTVPAAETLTNPNFDAMNYFDLSATFD
                     FGDKAEIYAGVNNVLDEDPPIVAGFGGYGNTFPATYDYAGMTVFMGVNVSF"
     gene            complement(6823..7812)
                     /gene="pdxA"
                     /locus_tag="VEW08_17400"
     CDS             complement(6823..7812)
                     /gene="pdxA"
                     /locus_tag="VEW08_17400"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HYJ42571.1"
                     /translation="MSIPIIAITMGDAAGVGPEIIMKALASPVVRAFCRPLVIGDAER
                     LRLAGELVGSRLKVESLDDPSEAAYGHGNVECIDLNLIPRDLPFGKTSAVAGEAAYRF
                     IERAVRIVECGAAQAICTAPLSKEALHAAGHRYPGHTELLASLTNTAEVSMMLVAPRL
                     RVIHVTTHMGLLDAIERIDAPLVERVIGRAHDTLVRAGVTNPRIGVCGINPHAGENGL
                     FGRGEEAGKIVPAVERARGRGWNVTGPLPADTLFFLAARGDYDIVVAMYHDQGHGPIK
                     VLGLESGVNITVGLPVVRTSVDHGTAFDIAGKGVADERSLIEALRQAAELVPR"
     gene            complement(7809..9080)
                     /locus_tag="VEW08_17405"
     CDS             complement(7809..9080)
                     /locus_tag="VEW08_17405"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HYJ42572.1"
                     /translation="MEPRWFIIADDLTGAADTASAFARRRLPATVVWGEETSDSHPDL
                     IALAYDAGTREFDAVEAARRHRDALHRFLRPGMHVYKKIDSTLRGNPAEEVAAMLDVL
                     LAQQSDARVVLTTAFPALRRTVRDGLVHVHGIALPFTEFWPEERDPELANLVNMLESA
                     GVHSRLLSLSSVRSDPAALSSALARVATSAGRPAVTVCDAETTDDLERIATASLDAGV
                     TCFVGSAGLAHALAKWVARNSMRRITTPMCAGEKRGALVVVGSRASASRAALSPVLAL
                     PNVFGISVQSSMLSGDLQSAAHAEIAQRVAAKLASGVDVVVDISQTAAGAGSDARLVT
                     ALASLLAPLARQASALVVTGGETAAAVLTRAGVSGIRLVDEIEPGIPLGLTLGEISVP
                     AVTKAGGFGNEECLKRIVSRLRFIRQTGAVA"
     gene            complement(9081..10043)
                     /locus_tag="VEW08_17410"
     CDS             complement(9081..10043)
                     /locus_tag="VEW08_17410"
                     /inference="COORDINATES: protein motif:HMM:NF015751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HYJ42573.1"
                     /translation="MKQVPIKRAIERVPGGLMVVPLLTGSLLATFLPGAPQFFGSFTG
                     ALFTGALPILAVFFVCTGASIDFRATPYILKKGGALFGVKVGIGILLGLVFGRLLGEA
                     PIGAGVFAGISTLAIVAAMNDTNGGLYMALMGQYGHPRDVGAYSVMSLESGPFLTMVT
                     LGVAGLSAFPWPTLLGGILPLLAGMALGNLDREMREFLSRAIPVLIPFFAFALGANLD
                     LGKVWLAGLLGLGLGVAVVVVTGIPLFFADRLTGGSGVAGVAAASTAGNAVAVPAIIA
                     SANPAYADAAAHATILVAASVVVTAILVPLVTAWAARRAGERTQ"
     gene            10083..10532
                     /locus_tag="VEW08_17415"
     CDS             10083..10532
                     /locus_tag="VEW08_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42574.1"
                     /translation="MNKYTRVAIVACLLAASFGTHAEDNKKAKVDCTMKFSLSGWSAF
                     YKRADGTGTVTCNNGQTANVKLEARGGGLTAGKSSIENGTGEFSNVKNIDEIFGKYVN
                     AEASAGAVKSSGAQAMTKGEVSLGLAGTGRGWDLGISFGKFTIKRAQ"
     gene            complement(10533..>11331)
                     /locus_tag="VEW08_17420"
     CDS             complement(10533..>11331)
                     /locus_tag="VEW08_17420"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HYJ42575.1"
                     /translation="ASSIAAALAFYCAFSLAPLLIIIVSIAGWIVGGELAYSYVGSQL
                     TMLFGKQSATLILEAMRSAQTTEGTWATIVSIVMLLVGASTVFAALESALQQVWGGRD
                     SRPHGWRSFLRSRLISFGFILAIGFLLLVSLTLTTALAALRGYVLRHFEGLVGVLASA
                     DFLMSILLGTGLVALMYRYLPARRLAWRHVITGALVTALLFHLGRWGIGVYLGRATQP
                     TAYGAAASFAALLLWLYYSAQIFLFGAEFTACLGQSRTPKALAGASA"
BASE COUNT         2179 a         3660 c         3550 g         1942 t
ORIGIN      
        1 cctcggccgc gccggtcgaa gatacgacgt tggcgctgtg gcagaagaac atggatatcc
       61 tcgcgacggg ttacttcctg atcgcgcgcc aggcctttca actgttcaag acgcaggcaa
      121 tcggcggatc catcgttttc attggttcga agaacgccct cgccgcctct ccgggggccg
      181 cggcgtactg caccgcgaag gccgcggaac tgcacctggc gcgctgcctc gcgctcgagg
      241 gcgcgccgct cggcattcgc gtgaacgtgg tgaacccgga tgccgtattg cgcggttcgc
      301 gtatctggca aggcgagtgg gccacgcagc gcgcggcgca gaacaagacc agcgtcgaca
      361 atctcgagac cgtgtatcgc gaacgcagcc tgctcaagcg cagcgtctac ccggaagacg
      421 tggccgaagc agtggcattc ttcgcttccg agcgttccgc caagtcgaca ggcaatatcg
      481 tgaacgtcga cgccggcaac gccggcgcgt tcacgcgtta aacgttcgag aggggcttca
      541 tgtccaaggc attcgccgat ccgcgcgatg cgagttacat cgcctcggcc aatcagccgc
      601 aggcggcagc actggccacc gactacaccg ccctgggcat ccagctcgaa cgccgcggga
      661 tcaagatcga tgacatcacc cgtgcggtcg ccaatttcaa ggtggcagtt ccttcgtggg
      721 gcgtcggcac gggtggcact cgcttcggcc gatttcccgg accgggcgag ccacgcaacg
      781 tcttcgagaa gctcgaggac tgcgcggtca tccacaaact gaccggcgcc actcccaccg
      841 tctcgctgca cattccctgg gaccgcaccg acgactacaa gggattgcgg gaacacggcg
      901 cggcgctcgg cctcgggttc gacgccatga actcgaacac tttccaggac caggtgggtc
      961 aatcgctgtc gtacaagtac ggcagcctca cccacgttga tgcggccgtg cgtgcgcaag
     1021 ccatcgccca caacatcgat tgcatcgcag cgggaaaggc cctgggctcc aaggccttga
     1081 cggtctggat cggcgatggc gcaaactttc cggggcagtc gcatctcggg cgcgcgttcg
     1141 agcgttacct cgaatcgacc aggtccatct acgcggcgct gcccgaagac tggcgcatgt
     1201 tcatcgaaca caagatgtac gagcctgcct tctatgccac cgtcattcag gattggggca
     1261 gctcactcat ggcggcgcag acgttgggcc caaaggcttt ctgcctggtc gacctgggcc
     1321 accatgcgcc gaacgtgaac atcgaaatga tcgtcgcgcg cctggtgcat gcacagcgcc
     1381 tcggcggctt ccatttcaac gactcgaaat atggcgacga cgatctcgac gccggatcga
     1441 tcgagccgtt ccgcctgttc ctggtgttca acgagctggt cgaagccgag ctcgcgaagg
     1501 cgcctaactt caggccggcg cacatgctgg atcagtccca caacgtgacc gatccgatcg
     1561 aaagcctcat gaccagcgcc atcgaactgc agcgcgccta cgcgcaggcg ctcatcgtcg
     1621 accgcaagtc gctgcacgaa taccgcatgg gcaacgatgt gctgatggcc gcccagacgc
     1681 tgaagcgcgg gttcaccacc gacgtgacgc ccatccttgc gcaggcgcgc gtgctgaaag
     1741 gcgccgccat cgacccggtc gggacgtatc gccagagcgg ttatcggggc aaatgcgcag
     1801 aaatgcggcc gggcaaacaa agcctcggtg gtggcatagt gtagccaggg gctgcgcccc
     1861 acgtcggaaa acgccgccga agcggcggcg tacatagaaa aatgacttat gcctgtgctc
     1921 aatagcatcg aggatcttcg ccaggaagct cgccgccgcg tgccgcgcgc catcttcgac
     1981 tacgccgacc gcggctcgta cgacgaagtc acgttcaacc gcaatctcgc ggaccttgcc
     2041 gcccttcagc tgcgccagcg cgtcatggtc gatgtgtcgc agcagcaact cgccaccacg
     2101 attctcggcg aggagtggac cattcctgtc ggtatcgggc ccaccggcct caccggcctc
     2161 ttccacgcca atggcgagat gctgggcgcg cgcgccgcgc aggctttcgg cgtgcccttt
     2221 tgtctctcca cgatgtccat ctgttcgatc gaagacgtcc gcagcgcggt gcaaaagccg
     2281 ttctggttcc aggtctatct gatgcgagat cgcagcttca acgccgaact gctcgatcga
     2341 gcgctcgctg ccaattgctc ggccatcatg ctcaccgtgg acatgcccgt gcagggcctg
     2401 cgccggcggg atccgaagaa cggcctgtcc attccgccaa agctcacact caagaacatg
     2461 ttcgaagtcg caatgcggcc gacgtgggcg ctcagggtgc tcaccggccg gcgccgcacg
     2521 ttcggcaatc tggaagcgcg catgaaaggc acgggcggtc tgaaaacgct gtccgagtgg
     2581 atcgcacaac aattcgatcc gacggtcacg tggaaagacc tcgcctggct gcgagagcgc
     2641 tggccgcgca agatcatcat caagggcatc ctcgacgccg aagacgcgag cctcgcctgc
     2701 cagcacggcg tggacgcgat ggtggtgtcc aatcatggcg gccgccagct cgatggagcg
     2761 cgctcgacga tatccgcact acccgaggtc gtcgaagccg tggcgggccg atgcgacgtg
     2821 ttgtttgacg gtggggtgcg ctgcgggcag gacgtgctca gggcgctggc gctgggcgcc
     2881 aaaggctgcc tcatcggcaa agcgtttctc tatgcgctgg ccgcgcgcgg tgaagcgggc
     2941 gtcacgctcg cgctcgatat catgcgcaag gagctcgccg tcagcctcgc gctcaccgga
     3001 gccaacgacg tgcgcaaagt tacgcgcgac atactggtgg cacccggcgc ttgagaaaaa
     3061 ggtggaggtg gtgccgggca ccaaaaatcg tagttgagta aacgctgata cagatccctg
     3121 ttgtcagctc aactacgact tttggtgccc ggcaccacct cacgctcacg cgagggggcc
     3181 cgcgcttaga acgatacgtt cacgcccatg aaaaccgtca tgcccgcgta gtcgtaggtc
     3241 gcgggaaacg tgtttccgta gccgccaaaa ccggcaacga tcggcggatc ctcgtcgagg
     3301 acgttgttca cgcccgcgta gatctcggcc ttgtcaccga agtcgaacgt cgccgaaagg
     3361 tcgaagtagt tcatcgcgtc gaagttcgga ttcgtcagcg tctctgccgc gggaaccgtc
     3421 ccgacatgcg aggtcttggg aagcacgatt ctgtcggtgg tgacgctgtc gatgaagcga
     3481 tgtctcaggc tcacgcccag ggggcccgtg gtccaggtga agcgggtaac acccttgtat
     3541 tgcggaatcg gctcaccgca ggtcgaacca taagcaccga cacagcggtt cttgttagcc
     3601 ggcacggcct gcatgggagt gatcgtgaac ttgtcggtat aggtccactg cgtgccgatg
     3661 tcgaagcggc tgccgccttc catgccaaag cccacatccc agccataacg cgcgcccagg
     3721 tcgtatccga tcgtcgccaa tcggccgatg ttcgcctgca gcacatcgag ggagtacgga
     3781 accgtaattc cacccgaggc cgggttgcga tggaccgcct ggcagaagtc gctgccagca
     3841 tcctggaccg tgaagtagca caggttcagg gtgttctgcg caccgccgcc gagctgcgca
     3901 atggccccat tgagcttgat gtcgaagtaa tcgagggtca ccaccaggtc gtccacgaac
     3961 gtgggcgtca gcacgacgcc taacgtagtc gtgtccgagc tttcttcatg caggtcaaca
     4021 ttgccgcccg agcgcgtggg aatgatgttg tccggctgca cgccggccgt gaacacggat
     4081 gcagcgggca caccggttgc ttcgcacagc gcccgcacgg cggccgtacg gttcgcggta
     4141 ttccggctcg agcatgggtc ggtcagcgtc tggaagttca gctgcaggcc accgtacagg
     4201 tcaccgatgt tcggtgcgcg aatggcgcgc tggaattggc cgcggaatcc gaccgactcg
     4261 ttgactcgcc agtcgaggcc gtacaggtac gtttcgactc tgcccacgcc gtcgaggtcg
     4321 tagtccgaac tgcgatagcc accattggcc gtgaggctct gcacgaacgg cagatcagcc
     4381 aggatcggca cgcgcagttc ggcgaaaatc tctttcgccg tgacctcgcc tgcggtcgga
     4441 agtcccggat tgaagccgat cacgtcgccc gaacgcaggt attcgtccgg gatgtactcc
     4501 gcttcggcac tgcgccactc gaggccgacc gagaagttga ccgcgccggc aggcaagtcg
     4561 aaggcctcgc cgccgagagt ggccacggcc accttctgct ccgcattcgt cgtgttggtg
     4621 gcgttgatct tgatggcgtt caccgccgcc gtggaaaggt tctgcccgaa gatgttagcc
     4681 accggaggat ttcccccgct ggagagcaac gcggcagcgt agcggctgcg cgaggcggcg
     4741 ccttcctggc ggctgctgtc ctcggagcgc gagaacgtga aatacacgtc gtagttcaag
     4801 ccacggagga acttgtccga ggcatcaccg agatcgccct tcatgccaag tgccatgcgc
     4861 cagacgttgt gatcatcgac gttgtgtcgg aaaggcagct ccaccaggcg tcgtcccgcg
     4921 gtgagaatgg ccaacccgtc gttcggagtg gtcgtgtacg cgatggggcc ctgctgcagc
     4981 gtccttggcc ccgtttcggc cgcatccagc gcaaccagcg ttgcgcgcat gttggcgtcc
     5041 agatacggat tgttggtgtt gaagaggaag tcgccgccga tgttcgcctg tgtgagctgc
     5101 tgatcgacgc ggttgttgct gaagtggaac tccatgtaac ccgtggcctt cggcgcgaat
     5161 tcgtagtgcg tgaatgcatt gagcatccag cgttcctgcg gcacttgcag atagttgaac
     5221 tgcgtcaggt tgaagtcatc cgccggccgg ttcaccagcc gctggctgcc ttgggcgccg
     5281 gcatcattga acgtgaagcc gttggaaccc aatcccgcaa gtccggcagc cgccagctgg
     5341 gtctgcgtgg ccgccagctg cgctggcgtg agcacgaagc gcccattggg aatgtcgccg
     5401 ctccctgaga agatgaatcc catcctgccg cccgatgtcg cgcagttggc ggcattcgag
     5461 ccattggcga cgccgatcag gctgtcactc caggagtcgg ctgtgacgca gccttcgccg
     5521 tacggaagct ccgcccagtc accgcgctcg tggcgcgtga atccttcgcg cttgtagtag
     5581 ttcagcgaga caaccgcgtt gccgcggcct tcggcgaaat taccgccaat cgtgagatcc
     5641 gtgctgaagg tcggcgtgct ggtggaactg tcgaaaccat actgcccgcc gattttcacg
     5701 ccttcgaagt catccttcat gatgaagttg acgacacccg tgatggcatc cgagccatac
     5761 accgccgaag aaccgccggt gacgacttcc gtgcgttcga tcaacgcggt tggaatggta
     5821 ttgagatcgg tcacctgctc cgggccgaag atggcgaaac gtcgaccgtt caccagcgtc
     5881 aggctgcgcg tcggaccgaa gccgcgcagg ttgacgtagg ccgcgccggc cgctgaaccg
     5941 gcgggaacgg tgtttgccgt cgcaccaccg ttggttgccg caacgaactg cggctggtcg
     6001 ttgagcacct tgtcgatgct cgtcgcgccc gagagtgtga tctcgtcggc gtcgatgatc
     6061 atgagaggtg tcgaagcatc cgtgacgtca cgcgcgatgc gcgatccggt gacgacgatg
     6121 acatctccct cttccgcggg cggcgtgctc gggtcttgcg cgagtgcgct cgttgcgctc
     6181 cccaacgcga ggccggtcaa cagatagacg gccgcgcgtc ctttgcgggc ctggcccgca
     6241 aatcgtttgc tgttcatgat ttccccttcg ttttttaaga cttctgaccg aaacggggat
     6301 caaggttccg cgctctaccc cgctgctgcc cgatcatgta tgagcgaatt tgcttgagca
     6361 agaaagaaaa tcgatcttcc acgcgcgagg atggagatga acgcaccgca attcgttcag
     6421 acgcgggcat ttacgagcgt ccggacgccc gttggggatt agaggaaaag gtgtgggaga
     6481 gaggtggagc gccgccagcg aaggggtgct agcggcgctc cgattgtcga aaagcgcaca
     6541 gagcgcggac aaggtgcact tctcggactc agcagaactt cttttttgaa gctccagaaa
     6601 tcagcgggat gtaacagttg ttagcggtca caatcgatca ttactgctca gtaacaatca
     6661 attcagtgcg cgttctatca caaatccccg aatagctcga ccaaaaatcg tatttattgg
     6721 ctcagaaatc gctgaacggg agcctacaca gctgatatcg ctaccagcta gcactgcttt
     6781 accaatggta gatatttttg cccgcgacgc gcgggcgagt ccttatctcg ggacgagttc
     6841 ggctgcctgg cgcagcgcct cgatcagact tcgttcgtcg gcgacacctt tgcctgcgat
     6901 gtcgaaggcg gtgccatggt ccacagacgt cctcacgact ggcaggccaa cggtgatgtt
     6961 cacacccgac tcgaggccca gcaccttgat ggggccgtgg ccctggtcgt ggtacatggc
     7021 gaccacgatg tcgtagtcgc cccgcgccgc gagaaaaaac agcgtgtccg ccggtaacgg
     7081 gcctgtgacg ttccagcctc ggccgcgggc tctctccacc gccggcacga tttttccggc
     7141 ttcttctccg cgaccaaaca aaccgttttc acccgcgtgc ggattgatac cgcacacccc
     7201 gatccgcgga ttcgtcacgc cggcacgaac cagcgtgtcg tgcgcgcgcc cgatgacgcg
     7261 ctccaccagc ggcgcgtcga tgcgctcgat ggcgtcgagc aatcccatgt gcgtcgtcac
     7321 gtggatcacg cgcaggcgcg gcgccaccag catcatcgaa acttcggccg tgttcgtgag
     7381 cgaagccagc aactccgtgt ggccgggata gcggtgcccc gctgcgtgca atgcttcctt
     7441 gctgagcggc gcggtacaga tggcctgtgc tgcgccgcat tcgacaatgc gtaccgcacg
     7501 ttcgatgaag cgataggcag cctcgccagc caccgccgag gtcttgccga aaggcagatc
     7561 ccggggaatg agattgagat cgatgcactc gacgttgccg tgcccataag cggcttcgga
     7621 cggatcgtcg aggctttcga ccttgagccg gctgccgacc agttcaccgg cgagccgcag
     7681 ccgctcggcg tcgccaatca ccagcggtct gcagaacgcg cgcacgactg gcgacgccaa
     7741 cgccttcatg atgatttcgg gcccaacacc cgcggcatcg cccatcgtga tcgcgatgat
     7801 ggggatgctc atgccaccgc acccgtttgc cggatgaagc gaagcctcga gacgatgcgt
     7861 ttcagacatt cctcattgcc gaacccgccg gccttggtca ccgcgggcac cgaaatttcg
     7921 ccgagtgtaa ggccgagcgg aatgccgggc tcgatttcgt ccacgagtcg aatgcccgaa
     7981 acgccagcgc gcgtgagaac agccgcggcg gtttcgccgc cggtcacgac cagagcgctc
     8041 gcctgacgag ccaggggtgc aagtaggctt gcgagcgcgg tcacgagcct ggcgtcactg
     8101 ccagctccag ccgctgtttg ggagatgtcg actacgacgt cgacaccgct ggcgagcttc
     8161 gcggcaacac gctgcgcaat ctcggcatga gcagcggatt gcaaatcccc cgacagcatc
     8221 gaggactgaa cgctgatgcc gaagacattc gggagcgcca gcaccggact caaggcggcg
     8281 cgcgacgcgg acgcccgcga tcccaccacg acgagcgcgc cgcgtttttc gcccgcacac
     8341 attggggtgg tgatccggcg catgctgttg cgtgccaccc atttcgcgag cgcgtgcgcg
     8401 agacccgcgc tgccgacaaa acaggtgacc ccggcgtcga gacttgccgt ggcgattcgc
     8461 tcgagatcat ccgtggtttc ggcatcgcaa acggtgaccg caggcctgcc tgcagaagtc
     8521 gcgactcgcg cgagcgcgga actcagcgcc gctggatccg accgcacgga cgacaacgac
     8581 aacagacgtg aatgcacgcc ggcgctctcc agcatgttca cgaggttggc gagttcggga
     8641 tcgcgttcct cgggccagaa ctccgtgaac ggcagcgcaa tcccgtggac gtggaccagg
     8701 ccatcgcgga cggtccggcg caaggcggga aacgctgtcg tcagtacgac tcgcgcgtcg
     8761 ctctgctgcg caaggaggac gtcgagcatc gcggcgacct cctccgccgg atttccccgc
     8821 agcgtcgaat cgatcttctt gtacacgtgc atgcccggac gcaggaaccg gtgcaacgcg
     8881 tcgcggtgac gacgcgcggc ctctacggca tcgaactcac gggtgccggc gtcgtaggcc
     8941 agcgcaatca gatccgggtg cgagtcggaa gtttcttcgc cccacaccac ggtcgccggc
     9001 aggcgccggc gcgcaaaggc actcgcggta tcggcggcgc ccgtcagatc gtccgcaatg
     9061 atgaaccagc gcggctccat ctactgtgtc ctctcgcccg cgcggcgcgc ggcccaggcc
     9121 gtgaccagcg gaaccaggat ggccgtgacc acgacgctcg cggccacgag gatcgtcgcg
     9181 tgcgcggcgg catcggcgta ggccggattc gcgctcgcga tgatggccgg cacggcgacc
     9241 gcattcccgg cggtggaggc cgcggccacg cccgcaacgc cgctgccgcc ggtcagacga
     9301 tcggcgaaga atagcgggat gccggtgacc acgaccaccg ccacacccag ccccaggcct
     9361 aacaacccgg cgagccacac cttgccgaga tcgagattgg cgccgagcgc gaacgcgaaa
     9421 aagggaatga gcacgggaat cgctcgactg aggaattcgc gcatttcgcg gtccaggttt
     9481 ccgagcgcca tgccggcgag cagcggcagg atgccgccga gcagtgtcgg ccacgggaac
     9541 gccgaaaggc cggcgacccc cagcgtcacc atcgtgagaa aaggacccga ttccagcgac
     9601 atcacgctgt acgcgccgac atcgcgcggg tggccgtatt gccccatcag ggccatgtac
     9661 aggccgccat tggtgtcgtt catcgcggcc acgatcgcga gcgtcgaaat tcccgcgaac
     9721 acgccggcgc cgatcggcgc ctcaccgagc aaccgcccga acaccaggcc taacaagatt
     9781 ccgatgccca ccttgacgcc gaacagcgcg ccgcccttct tgaggatgta cggcgtggcg
     9841 cgaaagtcga tgctggcgcc agtgcacacg aagaacaccg cgagtatcgg cagcgccccg
     9901 gtgaacagtg cgccggtgaa cgaaccgaag aactgcggcg caccggggag aaatgtagcc
     9961 agcaatgagc ccgtcagcag gggcacgacc atcaggccac cgggcacccg ctctatggcc
    10021 cgcttgattg gaacctgctt cattcttgcc aacccccgcc ggcacgagta gcgtgccgcc
    10081 ccatgaacaa atatacccgc gttgcaatcg tcgcttgcct tctggccgcc agcttcggca
    10141 ctcatgccga ggacaacaag aaggccaaag tcgattgcac catgaagttt tccttgagtg
    10201 gctggtcggc tttctacaag cgcgccgatg gcacggggac cgtcacctgc aacaacggtc
    10261 agaccgccaa cgtgaagctc gaggcacgcg gcggcggcct caccgccggc aaatccagca
    10321 tcgagaacgg cacgggcgaa ttctcgaacg tgaagaacat cgacgaaatc ttcggcaagt
    10381 acgtcaacgc cgaggcgagc gcgggtgccg tgaagtcgtc cggcgcacag gcgatgacca
    10441 agggcgaagt ctcgctgggc ctggccggca ccggccgcgg ctgggacctc ggcatctcct
    10501 tcggcaagtt caccatcaag cgcgcccagt aatcacgcgc tcgcgcccgc gagcgccttt
    10561 ggcgtgcgcg actggcccag gcaggccgtg aactccgcgc cgaacagaaa gatctgcgcc
    10621 gagtaataca gccacagcag cagcgcggcg aatgaggccg ccgcgccgta cgccgttggc
    10681 tgcgtggcgc gccccaggta gacgccgatt ccccagcggc cgagatgaaa cagcaacgca
    10741 gtcaccagcg cgcccgtgat cacgtggcgc caggccaggc ggcgcgccgg tagatagcgg
    10801 tacatgagtg cgaccagtcc tgtgccgagc aggatcgaca tcagaaaatc cgccgacgcc
    10861 aggaccccga ccaggccctc gaagtgtcgc agtacgtagc cgcgcagcgc cgcgagcgcc
    10921 gtggtcaggg tcagcgagac gagcagcagg aaaccgatcg cgagaatgaa cccgaacgag
    10981 atgaggcgcg accgcaggaa tgatcgccag ccgtgcggac gggaatcccg tccaccccac
    11041 acttgttgaa gcgcgctctc cagcgccgcg aacacggtgc tcgcgcccac caacaacatc
    11101 acgatgctga cgatcgtcgc ccaggtgcct tcggtggttt gcgcgctgcg catcgcctcg
    11161 aggatcaacg tcgcgctttg tttgccgaac aacatcgtca actggctgcc gacgtacgaa
    11221 tacgcgagct cgccgccgac gatccacccg gcgatcgaga cgatgatgat gagcaaggga
    11281 gccagcgaga aggcgcaata gaacgcgagt gccgcggcga tgctcgacgc c
//
LOCUS       DASQJS010000442         4703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3434862, whole genome shotgun sequence.
ACCESSION   DASQJS010000442 DASQJS010000000
VERSION     DASQJS010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3434862"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VEW08_17425"
     CDS             complement(<1..713)
                     /locus_tag="VEW08_17425"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HYJ42576.1"
                     /translation="MKSAGQLLFRRSPKLPVVLSTEIAECGLACLAMVAAYHGHDADL
                     NTLRQRFSMSMSGASLRGLMKLADHLGLSTRALRVEMSALGRIVLPAIIHWDMNHFVV
                     LESVSRNSATIHDPSRGRVKMSLQEFSNHFTGVALELAPSESFEKVRERQRLRITDLF
                     SRISGHRLAISYVLLLSIGLQLVAFVLPFQMQLVVDKAIGANDAGILLIVAIGFGGLT
                     LLNAVISGLRNWTLQLLGS"
     gene            complement(710..1258)
                     /locus_tag="VEW08_17430"
     CDS             complement(710..1258)
                     /locus_tag="VEW08_17430"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HYJ42577.1"
                     /translation="MHTEVEAAATPLFRNEAVRFQAARLQGEVFLKIRSPWLVLGFVC
                     AAIAIVVVAAAAMLTYDRKEHAAGALVFAEQGHLEAELRIAPTAIGFIKPGQLVRLRY
                     DAFPDRKFGQAQGKIASISSLPTGDGSYRARVTLERDYVQAFGERVALRPGMSFSGEI
                     VVEQRSLLEWLFEPLYALGARA"
     gene            complement(1242..2525)
                     /locus_tag="VEW08_17435"
     CDS             complement(1242..2525)
                     /locus_tag="VEW08_17435"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HYJ42578.1"
                     /translation="MSTLNREREFVLCTLGTQGDISPFLAIGSALAARGHRVTILSNE
                     HWREQVAACGAAFVAIAPRDPPQSGRDNFAFFHSNVLPSFHESFRYIEQRVVRGARVV
                     VLFKAGMLGAQCAAEKLGLRHIRIALQPSAIRSVQRPAWPLTKLAQGRWGALARSTLI
                     PLIYRTTELLGRYRRVTNAYRRSHGLRPVGLGETNRTEDVMLLLCPRWFAMPQADWPA
                     NCRFAGFAFTPAGELDAELRDFIATRGPPLVFTPGTGVTDTQPFFRMASQVCRALAAP
                     GIFLSAGMHEQWLAENILCRRYVDLGALLPRARLLVHHGGIGTTAQALRAGIPQVVMP
                     KAFDQPDNAMRIAALRLGGVVLSHRVADEAIVDLCRATLADQTLRERLVIAARDIRQG
                     CAATEVANVAETLALQDGVSQIAPRQQSASHAHGG"
     gene            2607..2801
                     /locus_tag="VEW08_17440"
     CDS             2607..2801
                     /locus_tag="VEW08_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42579.1"
                     /translation="MAGNGVKELARDGTPGGVTASATFVPPDVLSLGLEPLDTPLVIG
                     QRGLAELLGDLAVATDDLSS"
     gene            2816..4036
                     /locus_tag="VEW08_17445"
     CDS             2816..4036
                     /locus_tag="VEW08_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42580.1"
                     /translation="MAVLIVSMPNDYESVAVQWALRKLGTECDVLYTCDLAGGAEWSY
                     RLDAPDRLDTDFRGTSRHFDFQRYSTVWMRRPNIVVPRPALTDRFEQSAAERDCSQFM
                     RSMFARIERGKFVASHFGATRTCTSKPFQFSVAREVGLPLPPTLISNSPQRILRFFHE
                     HGGRMVYKPLTANAFLLADGNTTFAPTTLIEDEALLHDSDLISSPGICQAVIEKAAEV
                     RVTILGRSVFALEKSFPHRSTALHVDWRGMYTGAVFREHTLPAPLIEQSVRLLRELGL
                     VMGMFDFILDREGNYHFLEVNPQGQFLWADQLAGIQLNQLEGMAQFLLSRDPDFRYAR
                     EDRVSCAAFDSHGNREELRAREQAEHFGDVLTFQHGAVSFPVYIPFALSPQAIAAQVE
                     SVAPMSAGGISSTA"
     gene            4106..4300
                     /locus_tag="VEW08_17450"
     CDS             4106..4300
                     /locus_tag="VEW08_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42581.1"
                     /translation="MSLLQKTIAELRELQHEEIELVGGGGGPTGESTYCPSSSWIQTP
                     VGPSFVNDDGSGDSSPDWAF"
     gene            complement(4378..>4703)
                     /locus_tag="VEW08_17455"
     CDS             complement(4378..>4703)
                     /locus_tag="VEW08_17455"
                     /inference="COORDINATES: protein motif:HMM:NF021041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HYJ42582.1"
                     /translation="SAVDWLRRSQVSGVRLEKVMLEKPVQYQYHLATFDYRLVDDANA
                     PPLWARFYDLDDNSIVLANRDSVRVKNYSEIHPERRSGYSWFGTWPAALLNEEYPAWR
                     NRHPR"
BASE COUNT          896 a         1543 c         1441 g          823 t
ORIGIN      
        1 tgcgagccca gcagctgcag cgtccagttg cgcaggccgc tgatcacggc attgagaagc
       61 gtgagccccc caaaccctat tgccactatc aacagaatgc cggcgtcgtt ggcgccgatg
      121 gccttgtcga ccacgagctg catctgaaac ggcagcacga atgccaccaa ctgcagtccg
      181 atggacaaca gcagcacata cgatatggcg agccgatggc ccgaaatgcg cgagaacagg
      241 tcagtgatac gaagtcgctg tcgctcccgg accttctcga agctctcgga gggcgcaagc
      301 tcgagcgcca cgcccgtgaa gtgattggaa aattcctgca acgacatctt cactctgccg
      361 cggctcgggt cgtggatggt cgccgagttg cgcgatacgg actcgagtac cacgaagtgg
      421 ttcatgtccc aatggatgat ggccggcagc acgatgcggc cgagcgcgct catctccacg
      481 cgcaacgcac gcgtcgacag acccaggtgg tcggcaagct tcatgaggcc gcgcaggctc
      541 gcgcccgaca tggacatcga aaaacgctgc cgcagcgtgt tcaggtcggc atcgtggccg
      601 tgataggccg cgaccatggc gagacaggca agaccgcatt ccgcaatctc cgtggacagc
      661 accacgggta gtttggggct gcgcctgaac agcagttggc cagcggattt catgcgcgcg
      721 cacccagcgc atacaacggc tcgaacagcc attcgagcag tgagcgctgc tcgacgacga
      781 tctcgcccga gaacgacatg ccgggccgca acgcgacacg ctcgccgaag gcctgcacgt
      841 agtcccgttc cagcgtgacc ctcgcgcgat aagacccatc gcccgtgggc aatgaggata
      901 tcgacgcgat ctttccctgg gcctggccga attttctgtc gggaaaggcg tcatagcgca
      961 ggcgcaccag ttgccctggc ttgatgaagc cgatggccgt gggcgcgatc cgcaactccg
     1021 cttcaaggtg tccctgctcg gcgaacacca gcgcacctgc ggcatgttcc ttgcggtcat
     1081 aggtgagcat ggccgcggcc gcgacgacga caatggcgat cgccgcgcat acaaacccga
     1141 gcacgagcca gggcgaacgg atcttgagaa acacttcccc ttgcaagcgc gcggcttgaa
     1201 atctcaccgc ctcattgcgg aacaacggcg tggccgctgc ttcaacctcc gtgtgcatga
     1261 ctggcactct gctggcgggg cgcaatctgt gacaccccat cctgcaacgc aagcgtctcc
     1321 gccacgttcg ccacctcggt tgcagcgcat ccttgccgaa tatcccgtgc ggcgatcacg
     1381 agccgctcgc gcagcgtctg atcggcgagt gtggcgcggc acagatcgac gatggcctcg
     1441 tcggccaccc ggtgcgaaag caccacccct cccagcctca atgccgcgat gcgcatggca
     1501 ttgtcaggct ggtcgaaggc cttgggcatg acgacctgcg ggataccggc gcgcaacgcc
     1561 tgggcggtgg tgccaatgcc gccgtgatgc accaacaggc gcgcgcgggg cagcagcgcg
     1621 ccgaggtcca catagcgccg gcacaggatg ttttccgcca gccattgctc gtgcattccg
     1681 gcgctcagga atatgccggg cgcggccagt gcgcggcaca cctggctcgc catgcgaaag
     1741 aagggctgcg tgtccgtcac accggtgccc ggcgtgaaca ccagcggtgg cccgcgcgtc
     1801 gcgatgaagt cgcgcaactc ggcgtcgagc tccccggccg gcgtgaaggc aaagcccgca
     1861 aagcggcagt tagccggcca gtcggcttgc ggcatcgcaa accaccgcgg acacagcagc
     1921 agcatcacat cctcggtgcg attggtttct cccaggccca ccggccgcag cccgtgcgag
     1981 cggcggtagg cattcgtcac gcgccgatag cgccccagca gctccgtggt gcgataaatc
     2041 aatgggatga gagtgctcct ggccagcgca ccccatctgc cctgcgccag tttggtcagc
     2101 ggccaggccg ggcgctgcac ggagcggatg gccgatggtt gcagtgcaat ccggatgtgc
     2161 cgcagcccta acttctccgc cgcacattgc gcaccgagca tgcccgcctt gaacagcacc
     2221 acgaccctgg cgccgcgcac cacgcgctgc tcgatgtagc ggaaagactc gtggaaggac
     2281 ggcaacacat tgctgtggaa aaacgcgaag ttatcgcgac cactttgcgg tggatcgcgc
     2341 ggggcgatgg ccacgaatgc ggcaccgcag gccgcgacct gctcccgcca atgctcgttg
     2401 gaaagaatcg tgacgcgatg tccgcgcgcc gccaaggccg agccgatcgc caggaaaggc
     2461 gagatatctc cttgagttcc caaagtacag agcacgaatt cgcgctcgcg gttcagcgtg
     2521 gacatacgac ctccatacgc tgctgtcacg aaaagtagtt tgctgacaga tacggcctta
     2581 ctactggagc aacggcgagg gatcgcatgg cagggaatgg cgtgaaagaa cttgcgcgcg
     2641 atggaacgcc aggcggtgtt accgcatctg caactttcgt cccgcctgat gtcctgtcgc
     2701 tcggactcga gccattggac acgccgctgg tcatcggcca gcgcggactt gcagagctgc
     2761 tcggcgatct cgcggtggcg accgacgacc tgagctcatg agcggaggaa actcgatggc
     2821 ggtactgatc gtttcgatgc ccaacgacta tgaatcggtc gccgttcaat gggccttgcg
     2881 caagctcggc acggagtgcg acgtcctcta cacctgcgat ctggcgggcg gcgccgaatg
     2941 gtcgtatcgc ctggatgcgc ccgacaggct cgacaccgat tttcgcggca cgagcaggca
     3001 cttcgatttt cagcgctact cgaccgtctg gatgcgccgg cccaatatcg tcgtcccaag
     3061 gccggcgctc accgaccgct tcgagcaatc cgccgccgaa cgcgactgct cgcaattcat
     3121 gcgttcgatg ttcgcgcgta tcgagcgcgg caaattcgtc gccagccact tcggcgccac
     3181 gcgcacctgc acctccaagc cattccagtt cagcgttgca cgcgaggtcg gtctgccgct
     3241 gccgccaacg ctcatcagca actcaccgca gcgcatcctg cgcttcttcc atgagcacgg
     3301 cgggcggatg gtctacaagc cgctgaccgc caatgccttt ctgctcgccg acggcaacac
     3361 cacattcgcg cccactaccc tcatcgagga cgaggcgctg ctgcacgaca gcgatctgat
     3421 ttcctcgccg ggcatctgcc aggccgtcat cgagaaggcg gccgaggtcc gcgtcaccat
     3481 cctcgggcgc agtgtattcg ccctcgaaaa atcatttccg caccgttcca ccgcgttgca
     3541 tgtcgactgg cgcggtatgt acaccggcgc tgtcttccgg gagcacaccc tgcccgcgcc
     3601 cctcatcgag cagagcgtgc gcctgttgcg cgagctcggc ctggtcatgg gcatgttcga
     3661 tttcatcctg gatcgcgaag gcaactacca cttcctcgaa gtcaacccgc aagggcaatt
     3721 tctgtgggcg gaccagctcg cgggcattca gttgaaccag ctcgaaggca tggcgcagtt
     3781 cctgctgtcg agggatcccg acttccgcta tgcgcgcgag gatcgcgtgt cttgcgccgc
     3841 cttcgattcg cacggaaatc gggaagagct gcgcgcgcgc gagcaggcgg aacatttcgg
     3901 cgacgtactc acctttcagc atggcgcagt gagcttcccc gtttacatac cgttcgcgtt
     3961 gtcgccgcaa gccatagccg cgcaggtcga gagcgtcgca cccatgtccg ccggtggaat
     4021 ttcgtccact gcctaggcag tggacggaca acgtgggcgg gaatcttcta ccgccgcatc
     4081 atcatgtgaa aaccaaggag agaacatgtc attgcttcaa aagaccattg ctgagctgcg
     4141 tgagctgcag cacgaggaaa tcgaactcgt cggtggcggc ggcggcccga cgggggagtc
     4201 gacctattgc ccctcgtcct cgtggataca gacgcccgtg ggtccgagct tcgtcaacga
     4261 cgacggcagc ggcgacagct cgccggactg ggctttctag tcgtgccctg acggcatcgg
     4321 ggcggaagcg ctccgatgcc gtcttgcctc cgagtcaatg cccgtgaggg cgcgatgtca
     4381 gcgcggatgg cgattgcgcc acgccggata ttcctcgttc aacagcgccg cgggccaggt
     4441 tccgaaccac gagtagccgc tgcgacgctc gggatgtatc tccgaatagt tcttcacgcg
     4501 cacgctgtcc cggttcgcga gcacgatgct gttgtcgtcg agatcgtaga agcgcgccca
     4561 taacggcggt gcgttggcgt cgtccaccag tcggtagtcg aatgtcgcaa ggtggtactg
     4621 atactggacc ggtttctcca gcatcacttt ctcgagcctg actccgctga cttgcgaacg
     4681 acgcagccag tcgacagcac ttt
//
LOCUS       DASQJS010000443        14159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3437005, whole genome shotgun sequence.
ACCESSION   DASQJS010000443 DASQJS010000000
VERSION     DASQJS010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14159
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3437005"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(410..3463)
                     /locus_tag="VEW08_17460"
     CDS             complement(410..3463)
                     /locus_tag="VEW08_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42583.1"
                     /translation="MKGLLLQAATAALVVACGGGGYGDDGGDAPPPAPPAPVFRDAQF
                     VDDTVSGLRFSVTGVGEGVTDGAGKFQFVEGRKIDFLAGGTANRILIGSAAPTYTTGA
                     LSFSLQDLDEVRAANGDAYLANLLRLMVLLDSNGDTTDGFQIDAAANTAISAAVTGTK
                     TLDFAASATAFAADATIAGLATALNRPLVGVDEALVRYQLLFRQSRSSSIALTSDDTR
                     AVVVNRQKASVSVIRVRNTDGSDAAQLLAEIPVGKEPRFVAIAPNDSRAYVTNAVDGT
                     MSVIDLTANTPVAMGAAIHVGVEPRGIAITPNGAYAFIAENTVGEVAVVRLSNNEVVA
                     SIHVGGNPYAVAISNDGDKNDNDERVYVTQLFGEVIDPARPDGFDDAKQGVVTSFRVG
                     EAIINAGTGATLTRLLLKPMASGFTADRRNFCRLTRSALEIAGTVKYFNSGPDGNNGT
                     GAAQLTKETFCPDVTSTNIEATGPIGRVAQNAYPNMLFGALLRGPNLYVPNVGAQPEP
                     PVNFNTNVQALVGVLSTVINEETPFSVNLNTYVPKEATPASPTTSLDKLFLNDIVAID
                     ADKRGRDFLIVSRGGNYVIRATLGTDFKLTTLDANNVARRFQTGNLPSGVVMARSGLR
                     AYVNNELNTSISVLNLQDNTVIARDIESSAPPAPGTQAHRNLVGKLAFFTALGIPDKL
                     DNNGDGQFDVALRDINPLAHRGKASNNAWSSCASCHDDGHADNVTWIFETGPRQTIPL
                     EGTFARNNLDDQRILNWSAVRGSNTDFNNNARGIQGGVGFATDVDGANKTTQVFNHGP
                     TKGISDSLDAMSEWVATVRAPIMPAIAGNTETNGRATFLTYCAGCHGGAKWTKSRTKG
                     LYLDNPLLQADPVGPAFFNGGPPKNDAGVELAGPQVVSVIRDGKGRLLMLDNVGSFNV
                     ASPIELRGAAAVDGQTTQGFPSLGAGGFNSPSLLGVTYSGPYFHDGSSHTLEDVAARH
                     RLGDAPATIASTLPAQDLADLLAFVRSIDDDTPTMPNSTDLFIAP"
     gene            complement(3653..5272)
                     /locus_tag="VEW08_17465"
     CDS             complement(3653..5272)
                     /locus_tag="VEW08_17465"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYJ42584.1"
                     /translation="MKSHSKFRVLLFPLFAALAACGSDEPFQVEDVGQAPDWNGSNAN
                     GGGSGGSGSGSSGTFLPASNFDQMCVAPRPGTTDVPGTSTDENNWLRSWTNDTYLWFS
                     EVNYPNPESYPTPAYFDLLKTNAVTSSGQPKDKFHFTYDTDDWQALSQGEEVASYGAL
                     FEILAPSPPRNIVVAFIQGSTPAEGVLFRGDKIIQVDGEDAVNGNTQAVVDALNSGLF
                     PDNDTQAHTFVVQNVAGTQRTVTITPAFIATDPVPDWTVFNTPTGDVGYILFNDHIAT
                     AEGWLFDAISDMVDAQVDDLIIDMRYNGGGYLDIASELAYMVGNTTLTNGRTFEKLVF
                     NSKYPSINPVTGETIVPTPFHNRSLGFDPSLAANTPLPTLNLGRVYVITGSGTCSASE
                     SVINSLRGVGVTVYQIGSTTCGKPYGFYPTDNCGTTYFSIQFRGENAANFGDYTDGFS
                     PANTTANIGTTVPGCSVADDFSHELFDPLEQRTAAALALRASNNSAAACPAASGASDP
                     RLTKTSFQNAPDRSLWVSRPAARMNRIMRRN"
     gene            5397..7445
                     /locus_tag="VEW08_17470"
     CDS             5397..7445
                     /locus_tag="VEW08_17470"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HYJ42585.1"
                     /translation="MNRLLRPFGLKLGPLQAGATYFPLHFLAVGIGLTVLSAPEGVGV
                     FWPAAGSLFAFLLLYPARYWGALFITSYVAELLAHTLLAPELPFSGAGLLFPVKFAAA
                     LVGVGIMQVAVRGPISFARLRHVLIFTGAALFSTLLCAVVAIALRGDLSFGERAYWLA
                     VQSWWIGDFLGALIITPMVLTVGFHGFALGVHARGGRAATLSAFGVLLAMLLAVFLRK
                     PGALSSPLDVPYIVYPVLVWIAMLSGPRRTALAAGITVAIASVATTRGFGPFDSPYQL
                     QTFLALCVLPVLLLQAVMAERDYAIEKARTSDERYRAFIANSSEAIFRCELAEPMPVT
                     LSADDQVGWVRKHGVVAECNTAFMVALGVKDAQNPVVGTRLGDHPTWSKVYIERIREA
                     IRNGYQVRNIEHVVHGPYGLDRVLLISMIGIVDSGHVLRFWGSGRDVTAMREAEAALA
                     QHDAQLRALATEITLAEERARRKLASELHDGPAQNLMGLGMQLAEIKRGIDDRHLLQR
                     MEEAEQVLADTTLQTRTLMLELAPPGLHESGLLEALRWLADRVSKQQRLLVAVEDDGN
                     PKPLEDQVTVLLFQTVRELLQNVVKHARSKRATVRCAVNNDVLSLDVIDPGVGFEVHS
                     IDRLPTRQGGFGLFNIRERLKLMGGSIDIHSIVGEGTTVRIRVPLKSQHGLFDEALRA
                     "
     gene            7464..8255
                     /locus_tag="VEW08_17475"
     CDS             7464..8255
                     /locus_tag="VEW08_17475"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HYJ42586.1"
                     /translation="MLIERIWAANSLRNFHYLIACAETGEALIVDPLNAAQCLASARA
                     RGFDIRQVLNTHEHRDHTDGNAGVVAATRARVLSHADAANVIGGVDRGLARGDVIKVG
                     RTVELEVLDTPGHTRSHVCLLAHGDAPALFCGDTLFNAGAGNCHNGGDPGLLYETFVN
                     QLARLPDITRVFPGHEYMARNLAFTLDREPGNADAAKALPMAKSQQPEDARITTLGEE
                     KRVNAFLRLQNPEIIARLRERFPEIGEKPDARTVFVRLRELRNSW"
     gene            complement(8256..8612)
                     /locus_tag="VEW08_17480"
     CDS             complement(8256..8612)
                     /locus_tag="VEW08_17480"
                     /inference="COORDINATES: protein motif:HMM:NF023903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3703 domain-containing protein"
                     /protein_id="HYJ42587.1"
                     /translation="MSSFAENIRPHVDAELRAATQDSQNGFTHLERAHVLGQGSTREH
                     VRVHWHMLRWAWRHRDAREFAGQLLRIVGAATKTFIGLVPAGNTGGTSVSAVRPMPVD
                     PELAAIIDKARGAAED"
     gene            complement(8623..9009)
                     /locus_tag="VEW08_17485"
     CDS             complement(8623..9009)
                     /locus_tag="VEW08_17485"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HYJ42588.1"
                     /translation="MITHSGGCHCGRVRFEVDAPAELSIDDCNCSMCARTGYLHLIVT
                     ADRFRITKGADALTTYTFNTGTAKHLFCATCGIKPFYVPRSHPDGFSVNARCIDSPTV
                     TGMKIRPIDGRNWEQQYPEGRGEFRA"
     gene            complement(9006..9350)
                     /locus_tag="VEW08_17490"
     CDS             complement(9006..9350)
                     /locus_tag="VEW08_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42589.1"
                     /translation="MLAETGHGVVRELFIAPVIGGLRARQIGVLVGCVIIFVIAWLTA
                     RWMGATTFRQQLKVGALWVILTLVFEFALGRATGMSWAHILADYDPGRGGYMVAGLGF
                     LFFAPWLTRKLR"
     gene            complement(9395..10015)
                     /locus_tag="VEW08_17495"
     CDS             complement(9395..10015)
                     /locus_tag="VEW08_17495"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HYJ42590.1"
                     /translation="MIFYDYYANVPEETLRAFVSKQELGHFVTVSADGQPHIGLYPFL
                     FLGEAIEIHLHRSDEQLKDLLTNKKCVFEVDEMHGTIPSTWIHPTNAMFATAYHRTVI
                     FECEAEVSEDAEVLAAQQRRLMAHYQPTGHTPETTEHAMYRGPFKEISALTLHVRARK
                     VKWKLAQNRSREQREKIIAQLRGRARPTDAGAADAMQWTLDYEAAN"
     gene            complement(10012..10389)
                     /locus_tag="VEW08_17500"
     CDS             complement(10012..10389)
                     /locus_tag="VEW08_17500"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HYJ42591.1"
                     /translation="MDRLFYICAGVAGFLGVAIGAFAAHALKASLPPDLLAVFETGVR
                     YQMYHVFALCAASWGWARWQSRAFVFAGWLFVAGIAVFSGSLYLLAITGSRWLGAITP
                     LGGLAFLAGWLCLAWGAATKMRP"
     gene            complement(10398..10742)
                     /locus_tag="VEW08_17505"
     CDS             complement(10398..10742)
                     /locus_tag="VEW08_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HYJ42592.1"
                     /translation="MKYRGSCHCGKVAFEVEGEITGAVSCNCSICSKRGSLLWFVQRE
                     QMKVTGESELTSYTFNKHVIKHRFCRHCGILPFGEGTDPKGVATAAINIRCLNDFEFD
                     KVPVHHYDGKKA"
     gene            complement(10744..11139)
                     /locus_tag="VEW08_17510"
     CDS             complement(10744..11139)
                     /locus_tag="VEW08_17510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HYJ42593.1"
                     /translation="MSSDEQQIRELVATWMSATRAGDIATVLTLMTDDVVFLVTGQEP
                     FGKEHFAAALKPSVTGAPMPKIDGHSEIQEVQVSGNFAYMWSRLNVEMTMSDGKVTQR
                     AGHTLSVFRKTGGRWQLARDANLLSPVRI"
     gene            complement(11148..12182)
                     /locus_tag="VEW08_17515"
     CDS             complement(11148..12182)
                     /locus_tag="VEW08_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42594.1"
                     /translation="MKLSALGLAIGAIAFGASTIYLSVQLKEERALADKTAETMASLN
                     ARIAELEKAREQRIAMTGQFGGVAMAQGEMAMGPPPPAAEKGEPKTEVTEAVSFGAPP
                     PRSEAFEKMMRTNLRANNKRLYADIGEKLGLNKEDTSRLIDMLTDQQVVNFESMRGTN
                     VDQAERLRQRDEARRESQTEIDNFLGASKAAALRDYQETIPARQEMEQLTRQLDGADA
                     SLSEEQQKRMLAALIEERKRIPMPQMSDSTTPEEYTKAYSEWQNDYNERVNAAARSVL
                     DTEQMTAYADYQQWQKEMREQMVTRRGGRGPRGATGGNFTYSAVAAPIMAETVIMVGP
                     PPEEKPRKPQ"
     gene            complement(12318..12836)
                     /locus_tag="VEW08_17520"
     CDS             complement(12318..12836)
                     /locus_tag="VEW08_17520"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HYJ42595.1"
                     /translation="MARNIEIKARIENVALITPKVAGLATEGPIEIAQDDTFFNCANG
                     RLKLRAFSNDAGELIFYRRVNQAGPKESFYLRSPTSSPETLRESLSLAYGQIGRIRKY
                     RTLFLVGRTRVHLDRVEGLGHFLELEVMLVDDESPETGIREASDLMDHLGIQPSQLID
                     GAYLDLLLAQQG"
     gene            complement(12852..13310)
                     /locus_tag="VEW08_17525"
     CDS             complement(12852..13310)
                     /locus_tag="VEW08_17525"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYJ42596.1"
                     /translation="MRKLLYLNSAQRYSDFALLLMRLFVGLFLIWGVWDNITDSARMQ
                     EFASFLAKHGFLSPKILAPVSVYLQLAIGLGFVLGLFTRWAGIVCVVHFAIAIAMVDR
                     LGGMRGIFPSGCLIVIGLYLATYGAGRLSMDAALRANELPRASGGVRFKL"
     gene            complement(13423..13767)
                     /locus_tag="VEW08_17530"
     CDS             complement(13423..13767)
                     /locus_tag="VEW08_17530"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HYJ42597.1"
                     /translation="MSNDKAFARTPEPPYYACIFTSTRTAVDEGYGAMADRMVELASQ
                     QPGFLGVESARGADGLGITVSYWASLEAISAWKANAEHRVAQATGHRKWYEHFETRVA
                     RVERAYSGPKTG"
     gene            complement(13776..>14159)
                     /locus_tag="VEW08_17535"
     CDS             complement(13776..>14159)
                     /locus_tag="VEW08_17535"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HYJ42598.1"
                     /translation="GSRFVPATQLGRAQVLQWMFFEQYSHEPYVATPRYICRHFAADH
                     PRRSELPERLAKGRAAFAVMEQHLSTRQFFVNERYSIADIALYAYTHVAEDGGHNLET
                     YPNLREWLGRVAAQPNYVPLLARPK"
BASE COUNT         2492 a         4365 c         4564 g         2738 t
ORIGIN      
        1 cgcgagcatt ccatcaggga gctctgttct tgatgcggtg ccgcgtagat aggtcccgca
       61 atagtcccct ggtccgatgt tccggatttc ccacggcgag atgatccacc ttcggtacgg
      121 gaactatgag cgctcgctct attcttacga gcatgacgcc acggggatcc gcctgaggag
      181 aacgaaaatg ggactggtca cggacgatct cacgagtttg ctcaccgacc cgaaagaaga
      241 gaccgaagaa aacccatttg gggtcggctt ctcctccgcg acgcgaacaa cgatgcaatc
      301 acgctggcat cacgccgtgt accgacttcg gaaatggctg aggaccactc tcacggctca
      361 cggcgaacac ggggacggcc tcacgcgttg aggaccgccc ccgtgtcttt cacggtgcga
      421 taaacaggtc cgtcgagttc ggcatggtcg gcgtgtcatc gtcgatgctg cgcacgaagg
      481 cgagcaggtc ggcgagatcc tgagcaggca acgtactggc gatggtcgcg ggcgcgtcgc
      541 ctagccggtg gcgcgctgcc acatcctcga gcgtgtgcga gctgccgtcg tggaagtacg
      601 gtcctgagta ggtcacaccc agcaacgacg ggctgttgaa gccgcctgcg cccaatgacg
      661 ggaagccctg cgtggtctgg ccatccaccg ccgcggcacc gcgaagctcg atcgggctcg
      721 ccacgttgaa cgaacccaca ttgtcgagca tcagcagcct gccttttccg tcacgaatca
      781 cgctaactac ctgcggcccg gccagctcga caccggcgtc gttcttcgga gggccaccgt
      841 tgaagaatgc cggccctacc gggtccgcct gcagcagtgg gttgtccagg tacaagccct
      901 tggtgcggct cttggtccat ttggcaccgc catggcaacc ggcgcagtag gtgagaaatg
      961 tcgcgcgacc gttggtttcc gtattgcctg cgatcgccgg catgatgggt gcacgcaccg
     1021 tggccaccca ttcactcatt gcgtcgagag aatcgcttat gcccttggta ggaccgtggt
     1081 tgaacacctg cgtggtcttg ttggctccat ccacgtccgt ggcgaagccg acgccgccct
     1141 ggatgccacg cgcgttgttg ttgaagtccg tgttggagcc gcgcaccgca ctccagttga
     1201 ggatgcgctg gtcgtcgaga ttgttgcgcg cgaacgtgcc ttcgagcgga atggtctggc
     1261 gcgggccggt ctcgaagatc catgtcacgt tgtcggcatg gccgtcgtcg tggcaggaag
     1321 cgcagctcga ccacgcgttg ttcgacgcct tgccgcgatg ggccagcggg ttgatgtcgc
     1381 gcagcgccac gtcgaattgc ccgtcgccgt tgttgtcgag cttgtcaggg atgccgagcg
     1441 ccgtgaagaa ggccagcttg ccgaccaggt tgcgatgcgc ctgcgtaccg ggcgcgggtg
     1501 gcgcactcga ttcgatgtca cgcgcgatca cggtgttgtc ctggagattc agcacggaga
     1561 tggatgtgtt cagctcgttg ttgacgtacg cgcgcaaacc gctgcgcgcc atgaccacgc
     1621 cactcggcag gttgccggtc tggaagcggc gcgccacgtt gttggcgtcg agcgtcgtga
     1681 gtttgaagtc ggtgccgagg gtcgcgcgaa tcacgtagtt gccaccgcga ctcacgatga
     1741 ggaagtcccg gccgcgcttg tcggcgtcga tggcgacgat gtcattgagg aacaatttgt
     1801 cgagcgaggt cgtgggcgat gccggcgtgg cttccttcgg cacataggtg ttgaggttca
     1861 cggagaacgg cgtttcctcg ttgatcaccg tgctcagcac accgacgagc gcctgcacgt
     1921 tggtgttgaa gttcacgggc ggctcgggct gcgcgccgac gttcggcacg tagagattcg
     1981 gaccacgcag cagcgcgccg aacaacatgt tcgggtaagc gttctgcgcg acgcggccga
     2041 tgggtcccgt cgcctcgatg ttcgtggagg ttacgtccgg gcagaacgtt tccttggtga
     2101 gttgcgcggc gccagtgcca ttgttgccat ccgggccgct gttgaagtat ttcacggtcc
     2161 ccgcaatctc gagcgcggag cgcgtcagcc tgcagaaatt gcgccggtcg gcagtgaagc
     2221 cggaagccat cggcttcaac agcaggcgcg tgagcgtggc gccggtgccc gcgttgatga
     2281 tggcttcgcc cacccggaac gaagtgacaa cgccttgctt cgcgtcgtcg aatccatcgg
     2341 gccgcgccgg gtcgatgact tcaccgaaaa gctgcgtgac atacacgcgc tcgtcgttgt
     2401 cgttcttgtc gccatcgttg ctgatggcga ccgcgtaggg attgccaccg acgtggatgc
     2461 tggccaccac ttcgttattg gacaggcgaa ccaccgccac ctcgcccacg gtattctccg
     2521 cgatgaaggc ataggccccg ttgggagtga tcgcaatgcc gcgcggttcg acgccgacat
     2581 ggatcgccgc gcccatcgcc accggcgtgt ttgccgtgag atcgatgacg ctcatggtgc
     2641 cgtcgaccgc gttggtgaca tacgcacggc tgtcgttcgg agcaatcgcc acgaaacgcg
     2701 gctccttgcc cacggggatt tcggccagga gctgcgcggc atcgctgcca tcggtattgc
     2761 gtacgcggat caccgagacg ctggccttct ggcgattcac cacgaccgcg cgagtgtcat
     2821 cgctggtgag ggcgatgctc gaggatcgcg actgtcgaaa cagcaattgg taacgcacca
     2881 acgcttcgtc gacgccgacg agcgggcgat tgagcgccgt ggcgagcccg gcaatcgttg
     2941 cgtccgcggc gaaggcggtg gcgctggccg cgaaatccag cgttttcgtg ccggtgactg
     3001 cggcgctgat ggcggtgttg gccgccgcgt cgatctgaaa tccgtcggtg gtgtcaccat
     3061 tcgagtcgag cagcaccatc aggcgcagga gattcgccag ataggcgtca ccattcgctg
     3121 cgcgcacttc gtcgagatcc tgaagactga aggaaagcgc cccggtggtg taagtcggtg
     3181 ccgcgctgcc gatgaggatt cggttggccg tgccaccggc gaggaaatcg atcttgcgtc
     3241 cttcgacgaa ctggaatttg ccggcgccgt cggtgacacc ttcaccgact cctgtcacgc
     3301 tgaatcgcag gcccgacacc gtatcgtcaa cgaactgcgc atcgcggaac accggggcgg
     3361 gcggggcggg cggcggcgca tctccgccat cgtctccata accgccacca ccgcaggcga
     3421 cgaccagtgc cgcggtcgct gcctgcagca gcagtccttt cacgacgcgc atcctgcgcg
     3481 aacccttcgg caacgtccct gcgttcatag agtctccgac aatccaagtt gtagtcggtg
     3541 ataagtggcg gcagttgccc gcacggtttc aattgaacgg aaaagaaaaa ggcgccgaag
     3601 cgcctttttc caggaaccat tcgggcgcgt cgggaaacgc gccgcgcggt tttcagtttc
     3661 tgcgcatgat gcgattcatg cgtgccgctg gcctcgagac ccacaggctg cgatcgggtg
     3721 cattctggaa cgacgtcttt gtcaggcgcg gatcagaagc accgctggca gccggacagg
     3781 cggcggcact gttgttgctt gcgcgcaatg ccagcgcggc ggcggttcgc tgctccagcg
     3841 ggtcgaacag ctcgtgcgag aaatcgtcgg cgaccgagca accaggcacc gtggtgccga
     3901 tgttcgccgt ggtgtttgcc ggcgagaagc cgtcggtgta gtcgccgaag ttggcggcat
     3961 tctcaccgcg gaactggatg ctgaagtagg tagttccaca gttgtcggtg ggatagaagc
     4021 catacggctt gccgcaggtg gtggaaccga tctggtacac cgtgacaccg acgccgcgca
     4081 ggctgttgat gaccgactcg ctcgccgagc aggtgcccga acccgtgatc acgtagacac
     4141 ggcccaggtt gagtgtcggt agcggtgtgt tcgccgccag cgaagggtcg aagcccagcg
     4201 atctgttatg gaacggggtc ggcacgatgg tctcgccggt caccggattg atcgacgggt
     4261 acttggaatt gaacaccagc ttctcgaagg tgcggccatt ggtcagcgtg gtgttgccga
     4321 ccatgtaggc gagttcgctg gcgatgtcga ggtagccgcc accgttgtag cgcatgtcga
     4381 tgatcagatc gtcgacctgc gcgtcgacca tgtcgctgat ggcgtcgaag agccagcctt
     4441 cggcggtggc gatgtgatcg ttgaacagga tgtaacccac gtcacccgtg ggcgtattga
     4501 aaacggtcca gtccggcacg ggatccgtgg cgatgaacgc gggggtgatc gtcaccgtac
     4561 gttgtgtgcc ggcgacgttc tgcacgacga aggtatgggc ctgggtatcg ttgtccggga
     4621 acaggccgga attcagcgca tcgaccacgg cttgagtgtt gccgttgacg gcgtcctcgc
     4681 catccacctg aataatcttg tcgccgcgaa acaggacgcc ttcggcgggc gtcgagccct
     4741 ggatgaaggc caccacgata ttgcgcggcg gcgagggggc gaggatttcg aaaagggcgc
     4801 cgtagctggc cacttcctca ccctgcgaca gcgcctgcca gtcgtccgtg tcgtaggtga
     4861 agtgaaactt gtccttgggc tgacccgaag acgtgacggc gttggtcttc agcaggtcga
     4921 aataggcggg cgtggggtag gactccggat tcgggtaatt gacttcgctg aaccataggt
     4981 acgtgtcgtt cgtccacgag cgcagccagt tgttctcatc cgtgctcgtt cccggcacgt
     5041 cggtggtgcc cggcctcggg gctacgcaca tctggtcgaa gttcgaagcc ggcaggaagg
     5101 tgcccgagct gccactgcca ctgccgccgc tgccgccacc gttggcgtta cttccgttcc
     5161 agtcgggggc ttgcccgacg tcttcgacct ggaaaggttc gtcactgccg cacgcagcca
     5221 gcgcggcgaa cagggggaat agtaaaaccc tgaatttgga gtggcttttc acgtggcaat
     5281 ccctcaacgg caagcttttt gaataccaat ggagcgcaat atgcactttg ttaagcaaac
     5341 aacaaagcgt cgcgcccaag ctacgctcca cgtctcgttc tatgatggtg acagtcatga
     5401 atcggctcct tcgaccgttc ggactgaagt taggccccct ccaggcgggg gccacctact
     5461 ttccgttgca ttttctcgcg gtgggcatcg gccttacggt cctcagtgcg cccgagggag
     5521 taggcgtgtt ctggcccgcg gccggcagcc tgttcgcgtt cctgttgctt tatccagccc
     5581 gttattgggg cgcacttttc atcaccagct acgtcgcgga gctgctggcc cacaccctgc
     5641 tcgcaccgga actgcccttt tccggcgcgg ggctgctgtt tcccgtgaag ttcgcggcgg
     5701 cgctggtcgg cgtcgggatc atgcaagttg cggtgcgcgg gccgatttcc ttcgcgcggt
     5761 tgcgtcacgt actcatcttt accggggcgg ccctgttttc aacgctgttg tgcgccgtcg
     5821 tcgcgattgc gttgcgcggc gacctgtctt tcggcgaacg cgcgtactgg ctggcggtgc
     5881 aaagctggtg gatcggcgat ttcctgggcg cactcatcat cacccccatg gtactgaccg
     5941 tgggcttcca cggctttgcg ctgggggtcc acgcgcgcgg cggacgcgcc gcaacgctgt
     6001 ccgcattcgg cgtgttgctg gcgatgttgc tcgcagtgtt cctgcgcaag cccggggcgc
     6061 tttcttcgcc attggacgtg ccctacatcg tctatcccgt gctggtgtgg atcgcgatgc
     6121 tgagcggccc gcgacgcacc gcgctggccg cgggcatcac cgtcgccatt gcgtcggttg
     6181 ccaccacgcg cgggttcggg ccattcgaca gtccgtacca gctgcaaaca ttcctggcgc
     6241 tgtgcgtatt gcccgtgttg ctgctgcagg cggtgatggc cgagcgcgac tacgcgatag
     6301 agaaggcgcg caccagtgac gagcgttatc gcgccttcat cgccaacagt tccgaggcta
     6361 ttttccgctg tgagcttgcc gaacccatgc cggtcacgtt atcggccgac gatcaggtcg
     6421 gttgggtgcg caaacacggc gtcgttgccg agtgcaacac cgcgttcatg gtggcgctcg
     6481 gtgtcaagga cgcgcagaac ccggtggtcg gcacgcgtct cggtgatcat ccgacctggt
     6541 cgaaggtcta tatcgagcgc atccgcgaag ccattcgcaa cggctaccag gtgcgcaaca
     6601 tcgagcatgt ggttcacggg ccttacggac tcgaccgcgt gctgctcatc tcgatgatcg
     6661 gcatcgtcga cagcggacat gtgctgcgtt tctggggcag cggccgcgac gtaaccgcga
     6721 tgcgcgaggc ggaagccgcg ctcgcccagc acgacgcgca gttgcgcgcg ctggccacgg
     6781 aaatcacgct ggctgaagag cgcgcgcgcc gcaagctggc gagcgaactg cacgatggac
     6841 cggcccagaa cctcatgggc ctcggaatgc aactcgcaga gatcaagcgc ggcatcgacg
     6901 accgccacct gttgcaacga atggaagagg cagaacaagt gctcgcggat accaccttgc
     6961 agacgcgaac gctgatgctg gagctggcgc ctccgggcct gcacgagagc ggactgctcg
     7021 aagcattgcg ctggctggcc gatcgcgtct ccaagcagca gcgactgctg gttgcggtgg
     7081 aagacgatgg caatcccaag ccactggaag accaggtgac cgtgctgctg ttccagaccg
     7141 tgcgggaact gctgcagaac gtggtcaagc atgcgcgctc caagcgagcc acggtgcgct
     7201 gcgccgtaaa caacgacgtg ctgtcgctcg acgtcatcga cccgggtgtc ggattcgaag
     7261 tgcattccat cgatcggctg cccacgcgcc agggtggttt tggactgttc aacatccgcg
     7321 aacgcctgaa actcatgggc ggcagcatcg acatccattc catcgtgggc gaaggcacca
     7381 cggtgcggat ccgcgtgccg ctcaagtcgc agcacggctt gttcgatgag gcgctgcggg
     7441 cctgagggca acaaggaagt gccatgctca tcgaacgcat ctgggcggcg aattcgctgc
     7501 gcaatttcca ctacctcatc gcctgcgccg aaaccggcga ggcgctgatc gtcgatccat
     7561 tgaacgccgc gcagtgcctg gcgtcggcgc gggcccgcgg cttcgacatc aggcaggtcc
     7621 tgaatacgca tgagcaccgc gatcacaccg atggcaacgc cggagtcgtc gccgccacac
     7681 gcgcacgtgt gctgtcgcat gccgacgctg ccaacgtcat cggcggtgta gatagaggcc
     7741 tggcgcgtgg cgatgtcatc aaggtgggtc gtaccgtcga gctcgaggtg ctcgacacgc
     7801 ccgggcatac gcgctcgcac gtctgcctgc tggcacacgg ggacgctccc gcgctgttct
     7861 gcggcgacac gctgttcaac gccggcgccg gcaactgcca caacggcggc gatcccggcc
     7921 tgctctacga gactttcgtg aaccagcttg cgaggctgcc ggacatcacg cgcgtatttc
     7981 cggggcacga atacatggca cgcaacctgg cattcacgct ggaccgtgag cccggcaatg
     8041 ccgatgccgc gaaggccctg ccgatggcga aatctcaaca gccggaagac gcccgcatca
     8101 cgacgctggg tgaagaaaag cgcgtgaatg ctttcctgcg attgcagaac ccggagatca
     8161 tcgcgcggct gcgcgaaaga ttccccgaga tcggcgagaa gccggacgcg cgtaccgtgt
     8221 tcgtgcgcct gcgcgagctg cgcaacagct ggtagctagt cttcggccgc gccgcgcgcc
     8281 ttgtcgatga tggcggcaag ttccggatcg accggcatcg ggcgaaccgc actcacgctg
     8341 gtgccgccgg tgtttcccgc gggcacgaga ccgatgaacg tcttggtggc cgcgcccacg
     8401 atgcgtagca gctggcccgc gaattcccgc gcgtcgcgat gccgccaggc ccagcgcaac
     8461 atgtgccagt gcacgcgcac gtgctcgcga gtggaaccct gcccgagaac gtgagctcgt
     8521 tcgaggtgcg tgaatccgtt ctgcgaatcc tgcgtcgctg cacgcagctc ggcatcgacg
     8581 tgcgggcgga tgttctcggc gaaactgctc atgtattgct cctcacgccc ggaactcgcc
     8641 gcggccttcc gggtattgct gttcccagtt gcggccgtcg atgggccgga tcttcatgcc
     8701 ggtaaccgtc ggcgagtcga tgcagcgcgc gttgacgctg aacccatccg gatgcgagcg
     8761 cggcacgtaa aacggcttga tgccgcaggt cgcgcagaag agatgtttcg cggtgccggt
     8821 gttgaaggtg taggtcgtca gcgcgtccgc gcctttcgtg atgcgaaagc gatcggccgt
     8881 cacgatgagg tgcaggtagc cggtgcgcgc gcacatggag caattgcagt cgtcgatgga
     8941 aagctccgcc ggcgcgtcga cttcgaagcg cacgcggcca cagtggcatc cgccggaatg
     9001 cgtgatcatc ggagcttcct cgtgagccag ggagcgaaga acaggaaccc taggccggca
     9061 accatgtatc cacctcgccc cggatcgtag tcggccagga tgtgcgccca gctcatgccc
     9121 gttgcacgcc cgagcgcgaa ttcaaacacc agcgtgagta tgacccagag tgcgcccacc
     9181 ttgagctgct ggcgaaaagt cgtcgcaccc atccagcgcg cagtgagcca cgcgatgacg
     9241 aagatgatga cgcagcccac cagcacgccg atctgacgcg cacgcagacc gccgatgacc
     9301 ggtgcgatga agagctcgcg gaccacgcca tggcccgttt cggcgagcat gatcaacatc
     9361 cacacgaggg tggctcgcgt ccacattggc gccgctaatt cgccgcttcg tagtcgagcg
     9421 tccattgcat ggcatccgca gctccggcgt cggtggggcg cgcacggcca cgcagctggg
     9481 cgatgatttt ctcgcgctgt tcgcggctgc ggttttgcgc cagcttccac ttcaccttgc
     9541 gcgcgcgcac gtgcagggtg agcgcggaga tttccttgaa tgggccgcga tacatcgcgt
     9601 gttcggtggt ctccggcgtg tggccggtgg gttgatagtg cgccatgagt cggcgctgct
     9661 gcgcggccag cacctcggcg tcctcggaca cctcggcctc gcactcgaaa atcacggtgc
     9721 ggtgataggc ggtcgcgaac atggcattgg tggggtgtat ccaggtcgag ggtatggtcc
     9781 cgtgcatctc atccacctcg aacacgcact ttttgttcgt gagcaggtct ttgagctgct
     9841 cgtcggagcg atgcaggtgt atctcgatcg cctcgcccag gaacaggaat gggtagagcc
     9901 cgatgtgtgg ttggccatcg gcgctgacgg tgacgaaatg gcccagctcc tgtttgctga
     9961 cgaaagcgcg cagggtttct tcgggaacgt ttgcgtagta gtcgtagaaa atcatgggcg
    10021 catcttagtt gccgcgcccc acgccagaca gagccacccg gccagaaacg ccaggccacc
    10081 tagcggtgtg atcgcgccga gccagcgcga accggtgatc gccagcagat agaggctgcc
    10141 ggagaacacc gcaatgccgg cgacaaacaa ccatccggcg aaaacgaacg cccgggattg
    10201 ccagcgcgcc caaccccacg aggccgcgca caacgcgaac acgtgataca tctggtagcg
    10261 cacgccggtt tcgaacaccg cgagcaggtc gggcggcaga ctggccttga gcgcgtgggc
    10321 cgcaaatgcc ccgatcgcca cgcccagaaa tccggcgacg cccgcgcata tatagaagag
    10381 tcggtccaaa tggccgctca ggcctttttc ccgtcgtaat gatgcaccgg caccttgtcg
    10441 aactcgaagt cgttcaggca acggatgttg atggcggccg tggcgacacc cttgggatcg
    10501 gtgccctcgc cgaacggcag gatgccgcag tgcctgcaga accggtgttt gatgacgtgt
    10561 ttgttgaatg tgtagctggt gagttccgac tcacccgtga ccttcatctg ctctcgctgc
    10621 acgaaccaca gcaacgagcc gcgcttcgag cagatggagc agttgcagga caccgctccg
    10681 gtgatctcac cttcgacttc gaaggcgacc ttgccgcagt ggcagcttcc tcgatatttc
    10741 atgtcaaatc ctcaccggtg aaagtagatt ggcgtcgcgc gcgagctgcc agcgcccgcc
    10801 ggtcttgcga aatacggaaa gcgtgtggcc ggcgcgctgg gtcacctttc catcggacat
    10861 cgtcatttcg acattgagcc tgctccacat gtaggcgaag ttgccggaga cctggacttc
    10921 ctggatttcg ctgtggccgt cgattttcgg catgggggcg cccgtgaccg aaggcttcag
    10981 cgccgcggcg aaatgctcct tgccgaaggg ttcctgcccg gtgacgagga acaccacatc
    11041 gtcggtcatc agggtcagca ccgtggcgat gtctccggcc ctggtcgccg acatccaggt
    11101 ggccacgagt tcgcggatct gctgctcgtc ggaactcacg cgttcaccta ttgaggcttg
    11161 cgcggttttt cctcgggggg cgggccgacc atgatgactg tctcagccat aatcggcgcc
    11221 gccactgccg agtaggtgaa gttaccgccg gtcgcgccac gcggcccacg accaccccgc
    11281 cgcgtcacca tttgctcgcg catttccttc tgccattgct ggtagtcggc gtaggccgtc
    11341 atctgttcgg tatcgaggac gctgcgtgcc gcggcattca cgcgttcgtt gtaatcgttc
    11401 tgccattccg agtacgcctt ggtgtactcc tcgggcgtgg tgctgtcgga catctgcggc
    11461 atcggaatac gtttgcgctc ctcgatcagc gccgcgagca tgcgcttctg ttgttcttcg
    11521 ctgaggctgg cgtcggcgcc atcgagctga cgcgtgagct gctccatctc ctgtcgggcc
    11581 ggaatcgttt cctggtaatc gcgcagcgcc gcggctttgg aggcgcccag aaagttgtcg
    11641 atttccgtct ggctttcacg gcgtgcttcg tccctctggc gtaatcgctc ggcctgatcg
    11701 acgttcgttc cgcgcatgct ttcgaagttc acgacctgct ggtcggtgag catgtcgatg
    11761 agcctgctcg tgtcttcctt gttgaggccg agcttttcgc cgatatccgc gtacaggcgc
    11821 ttgttgttgg cgcgcaggtt ggtgcgcatc atcttctcga aagcttcgct gcgcgggggc
    11881 ggcgcgccga atgacaccgc ctcggtgact tcggtttttg gttcgccctt ttcggctgca
    11941 ggcggcggtg ggcccatcgc catttctccc tgcgccatcg cgacgccgcc gaactgtccg
    12001 gtcatcgcaa tgcgttgttc ccgcgccttt tcgagttcgg cgatgcgcgc gttgagggag
    12061 gccatggttt cggcggtctt gtcagccagg gcacgctctt ctttgagctg taccgacaga
    12121 taaatggtgc tggcaccaaa ggcgatggcg ccaatagcca aacccagcgc ggataatttc
    12181 attgttatct cccttgctcc cggagcatga cgccattgtc cgcgatcggt gccgggccgg
    12241 gaaaccggga aaactccaca tgcccggccc accgaactca cttcgtcaaa ttgttggact
    12301 ccaatccggt cctgcgccta cccctgctgg gccagcagca ggtcgaggta tgcgccgtcg
    12361 atcaactgcg agggctgaat tccgaggtga tccatgaggt cactcgcttc gcgaattccc
    12421 gtttccgggg actcgtcatc caccagcatg acttcgagct cgagaaaatg gcccaggccc
    12481 tcgactcggt ccaggtgaac gcgggtacgt ccaaccagga agagagtgcg gtacttgcgg
    12541 atgcgaccga tttgtccgta ggccagcgat agcgattcac gaagcgtctc gggtgatgaa
    12601 gtgggggacc gtagataaaa agactctttg ggccccgcct ggttcactcg ccggtagaaa
    12661 atgagctcac ccgcgtcgtt cgagaaggcg cggagcttca ggcgaccgtt ggcgcagttg
    12721 aaaaaggtgt cgtcctgggc gatttcgatc ggcccctccg tggccagccc ggccactttc
    12781 ggggtgatca gcgcgacatt ctcgatgcgc gccttgatct cgatgttgcg ggccataccg
    12841 ccaacgcgcc gttacagctt gaaacgcacg ccgccgctgg cccgcgggag ctcattcgcc
    12901 ctgagcgccg catccatcga gagccggccg gccccgtagg tcgccagata caggccgatc
    12961 acgatgaggc agccggacgg aaatatgccg cgcatgccgc cgaggcgatc gaccatcgca
    13021 atggcaatcg cgaagtgaac gacgcagacg attccggccc agcgtgtgaa cagtccgagc
    13081 acgaacccca ggccgatggc cagctgaagg taaaccgaga ccggtgcaag gattttgggg
    13141 ctcaagaacc cgtgtttcgc gagaaacgac gcgaactcct gcatgcgcgc gctatcggta
    13201 atgttgtccc agacacccca gatcaggaac aggccgacga acaggcgcat cagcagaaga
    13261 gcgaagtcgg aatagcgctg cgcggaattc aggtacagca acttccgcat gagtcctcac
    13321 gtgcccggaa atgatgatca ggcgcggaag actaacggta cggggcgcga caaccaatag
    13381 ttgtcgcacc cgtgtgacga agccgtcgcc gcgtggcgac gtctaaccgg tttttggacc
    13441 gctataggcc cgttcgacac gcgccactcg cgtctcgaag tgctcgtacc acttgcgatg
    13501 gccggtcgcc tgggcgacgc gatgttccgc attggccttc caggcggaga tggcttcgag
    13561 cgacgcccag tacgaaacgg tgatgccgag gccgtcggcg ccccgcgcgc tttctacccc
    13621 gaggaacccc ggttgctgcg aagcgagctc gaccatgcga tcggccatgg caccgtagcc
    13681 ttcatccacc gccgtgcgcg tggaggtgaa aatgcacgcg tagtacggcg gctcgggagt
    13741 ccgggcaaaa gccttatcgt ttgacattga ggtgctcatt tcggccgggc gagcaaaggc
    13801 acgtaattcg gctgtgccgc tacgcgccct aaccactcac gcaggttggg ataggtctcg
    13861 agattgtggc caccgtcctc ggccacgtgc gtgtaggcgt acagcgcgat gtcggcgatg
    13921 ctgtagcgtt cgttcacgaa gaactgccgt gtcgacaggt gctgctccat gacggcgaat
    13981 gcggcacggc ccttggcgag gcgttccggc aactcggagc ggcgcggatg atccgcggcg
    14041 aaatgccggc agatgtaccg cggcgtggcc acgtacggct cgtggctgta ctgctcgaag
    14101 aacatccact gcagcacctg cgcgcggccg agctgggtcg ccggcacgaa gcgcgatcc
//
LOCUS       DASQJS010000444         6260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2804340, whole genome shotgun sequence.
ACCESSION   DASQJS010000444 DASQJS010000000
VERSION     DASQJS010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2804340"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1200
                     /locus_tag="VEW08_17540"
     CDS             43..1200
                     /locus_tag="VEW08_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HYJ42599.1"
                     /translation="MDREQGIKLMQDLLRRVVDKKASDLFITAGFPPAIKIDGEVRPQ
                     SERALTGEQSAVLVRSIMNDRQTKEFDATKECNFAIAPPGIGRFRVNTFVQQGHTGCV
                     IRLINSKIPTLEELELPPILKEVVLSKRVLCILVGGTGSGKSTSLAAMVGYRNEKTRG
                     HIVTIEDPVEYVHQHKGCVITHREVGVDTENWHTALKNTLRQAPDVILIGEIRDRETM
                     EYGIQFAETGHLVLATLHANSSNQALDRIINFFPDERREQLLMDLSLNIRALVSQRLV
                     PREAGAGRIAAMEIMLNSPLIQDLIFKGKVQEIKDVMSRSNRVGMKTFDQALYELYEQ
                     GFISYEDALRNADSKNELRLRIKLESKREVKNLDDGGTLSMVDEEQQGRSF"
     gene            1232..2350
                     /locus_tag="VEW08_17545"
     CDS             1232..2350
                     /locus_tag="VEW08_17545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HYJ42600.1"
                     /translation="MNEQFVDTIEVKEPASGAPMVLNTRPLFKLMVERKASDLFFTSN
                     SPIKVKIEGAILPINKQVLTPETVRQAAYGLMTPDQLESFKRELEIDFAISEPGLGRF
                     RVNVFYQRGYPAMVLRYISADMPKLDQLGLPEMFKDLIMMKRGLILMVGATGSGKSTS
                     IAAMINHRNETSSDHIVTIEDPIEFLHTNKRSIVNQREVGLDTKSFARALRGVMRAAP
                     DVILIGEIRDKETMEAAITLSGTGHLVLSTLHANNSAETMDRIINMFPHDQHKQVFLD
                     LSQYLRAILSQRLVVAKDGKRMAAIEVMINTPYISELVKKGDVSAIKEAIVASSERGV
                     QSFDVALLNLYKQNRIELEEALMNADSRSNLEAKINFG"
     gene            complement(2337..3089)
                     /locus_tag="VEW08_17550"
     CDS             complement(2337..3089)
                     /locus_tag="VEW08_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42601.1"
                     /translation="MFDWNYWLCEFEARVPRLAVARVPPNSVLTREERARIADSIGTF
                     QLGEQSEGRTLLRFAREFGARHSCPALGLVTELFIREEQHHAAQLREFMLAHGIVLKQ
                     RNWTDSIFRQIRRLAGFETAVTILVTAEMVGFVYYRALGRATQNTYLRTICRQMCADE
                     AVHLRYEAELLRTLRGARPRWLRGLVELAHRSLLAIAARVVFREHRPVLEFVGHDARS
                     FVKDCRSLYRTVLTAEGKRAISQRGRARLSRS"
     gene            3671..3743
                     /locus_tag="VEW08_17555"
     tRNA            3671..3743
                     /locus_tag="VEW08_17555"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            4057..5187
                     /locus_tag="VEW08_17560"
     CDS             4057..5187
                     /locus_tag="VEW08_17560"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HYJ42602.1"
                     /translation="MLGLKRLSVAQYERALVFRERSLERVLTRGVYWLWDPMARLTVQ
                     VYDATVPELVLPRADVLVAEARALLEPYVQIVELGDREVGLVYRNDRLTGVLAPGSRQ
                     LYWRGSVSVRVEVRDIANEYALDANTARVLTRAKGVAGASDAISTVEVPDTSVGLLIV
                     DGELREVLKPGLSAYWKYQRVLRVELVELRLQAMEVSGQEILTRDKVSLRVNLTALWQ
                     VTDAVKARATVSNFVDYVYKELQFALREAVGTRTLDELLGDKGALDREVSASAARLEA
                     SGLAVRSVGVKDVILPGEMKTILNQVVEAEKVAQANLIRRREETAATRSLLNTARLME
                     ENPTLLRLKELETLEKVTEKIGNLTVYDGLEGVLKNMVPRLS"
     gene            5227..>6260
                     /locus_tag="VEW08_17565"
     CDS             5227..>6260
                     /locus_tag="VEW08_17565"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HYJ42603.1"
                     /translation="MQNEHYETMNVEGGRHVKMWTRGVPVDEQAKAQLANIARMPFVH
                     SHLAVMPDVHVGKGATVGSVIATQGAIIPAAVGVDIGCGMMAVQTSLKASDLPESLSA
                     LRSRIERAVPHGSSKVRGRYDRGSWGITPNSVISRWRALSERCDAITAKYPKLKPKEP
                     QKQLGTLGGGNHFIEVCLDTDQTVWVMLHSGSRGIGNMIGQLFIELARQDMKKHFINL
                     PDRDLAYLVEGTEHFDDYVEAMTWAQDYAAENRRTMMDAVLRVMREELRAFQMGEVAV
                     NCHHNYCTRELHFGEELIVTRKGAVSAKSGELGIIPGSMGARSYIVRGLGNTESFQSC
                     SHGAGRVMSRT"
BASE COUNT         1258 a         1867 c         2028 g         1107 t
ORIGIN      
        1 tacaaagcca ccaacaaaga cgccttgtga ggagctgacc caatggatcg cgaacaaggt
       61 atcaagttga tgcaggacct tctgcgtcgc gtcgtcgaca agaaggcctc cgacctgttc
      121 atcaccgcgg gattcccgcc cgcgatcaag atcgacggcg aagtgcggcc gcagtccgag
      181 cgtgcgctga ccggcgaaca gtcggcggta ctggtccgtt ccatcatgaa cgaccgccag
      241 accaaggaat tcgacgcgac caaggaatgt aatttcgcga tcgcgccccc cggcatcggt
      301 cgcttccgcg taaacacctt tgtgcagcag ggccataccg gctgcgtcat ccgcctcatc
      361 aactccaaga tcccgactct cgaagaactc gagctgccgc ccattctcaa agaagtcgtg
      421 ctttcgaaac gcgtcctctg cattctcgta ggcggcacag gctcgggtaa gtcgacctcg
      481 ctcgccgcga tggtcggcta tcgcaacgaa aagacccgtg gccacatcgt gaccatcgaa
      541 gatccggtcg aatacgtgca ccagcacaag ggctgcgtga tcacgcatcg cgaagtgggc
      601 gtcgacaccg agaactggca caccgctctg aagaacacgc tgcgccaggc gccggacgtc
      661 atcctgatcg gtgaaatccg tgaccgcgaa accatggaat acggcattca gttcgccgaa
      721 accggccacc tggtgctcgc aacgctgcac gccaacagct ccaaccaggc gctcgatcgc
      781 atcatcaact tcttccccga cgagcggcgc gaacagctgc tcatggatct ctcgctgaac
      841 atccgcgcgc tggtctcgca gcgcctcgtg ccgcgcgagg cgggcgccgg ccgcatcgcc
      901 gcgatggaaa tcatgctgaa ttcgccgctg atccaggacc tgatcttcaa aggcaaggtc
      961 caggagatca aggacgtcat gtcgcgctcc aaccgcgtcg gcatgaagac cttcgaccag
     1021 gcgctgtacg aactgtacga gcagggcttc atttcttacg aagacgcgct gcgcaatgcc
     1081 gattccaaga atgagctgcg gctgcgcatc aagctcgaga gcaagcgcga ggtgaagaac
     1141 ctcgacgacg gcggcacgct gtcgatggtc gacgaagaac aacagggtcg gtcgttctaa
     1201 accgacgtaa tcaagagcgg atatgccgaa agtgaacgaa cagttcgtcg acaccattga
     1261 ggtaaaggaa ccggcgagcg gcgcaccgat ggtgctcaac acccggccgc tgttcaaact
     1321 catggtcgaa cgcaaggcca gcgacctgtt cttcacttcg aattcgccca taaaagtcaa
     1381 aatcgaaggc gccatcctgc ccatcaacaa gcaggtgctg acacccgaga ccgtgcgcca
     1441 ggcggcctat ggcctcatga ctcccgacca gctcgaaagc ttcaagcgcg agctggaaat
     1501 cgacttcgcc atctccgagc ccggtctcgg tcgcttccgc gtcaacgtgt tctaccagcg
     1561 cggttatccg gcgatggtgc tgcgctacat cagcgccgac atgccgaagc tcgaccagct
     1621 cgggctgccc gagatgttca aggacctcat catgatgaag cgcgggctca tcctgatggt
     1681 gggcgccacc ggctcgggca agtccacgtc catcgccgcg atgatcaacc accgcaacga
     1741 gacctcgtcg gatcacatcg tgaccatcga ggacccgatc gaattcctgc acaccaacaa
     1801 gcgctcgatc gtcaatcagc gcgaggtcgg cctcgacacg aagtcgttcg cacgcgcgtt
     1861 acgcggcgtg atgcgtgcag ctcccgatgt catcctcatc ggcgaaattc gcgacaagga
     1921 gaccatggag gcggcgatca cgctttccgg caccgggcac cttgttctgt ccacgcttca
     1981 cgcgaacaac agcgcggaga cgatggatcg catcatcaac atgtttcctc atgatcagca
     2041 caagcaggtg ttcctcgacc tgtcgcagta cctgcgcgcc atcctctcgc agcgactggt
     2101 cgtcgccaaa gacggcaagc gcatggcggc catcgaagtc atgatcaaca cgccctacat
     2161 cagcgaactg gtgaagaagg gtgacgtttc cgcgatcaag gaagccatcg ttgccagctc
     2221 cgagcgcggc gtgcagagct tcgatgtggc gctgctgaac ctctacaagc agaaccgcat
     2281 cgagctcgaa gaagcactca tgaatgcgga ctcgcgttcg aacctcgaag ccaagatcaa
     2341 cttcggctga gcctggcgcg cccgcgctga ctgatcgcgc gttttccttc cgcggtgagt
     2401 accgtccgat acagcgagcg gcaatccttt acgaagctgc gcgcgtcgtg accgacgaac
     2461 tccagcaccg gccggtgctc gcgaaacacc acgcgtgcgg cgattgccag gagcgaccgg
     2521 tgagccagtt ccaccaggcc acgcagccag cgcggtcggg ctccgcgcag cgtacgcagc
     2581 agctccgcct cgtaccgtaa atgtacggcc tcgtcggcgc acatctgacg gcagatggtt
     2641 cgcaggtacg tattttgagt cgcgcgtccg agtgcccggt aatacacgaa tccaaccatc
     2701 tccgcggtga cgaggatggt cacggcggtc tcgaatcccg cgagccggcg gatctgccgg
     2761 aaaatgctgt ccgtccagtt gcgctgcttg agcacgatgc catgcgccag catgaactcg
     2821 cgcagctgcg cggcatggtg ttgctcttcg cggatgaaga gctcggtgac caggccgagc
     2881 gccgggcacg aatgacgcgc gccgaattca cgcgcgaaac gcagcagcgt gcgtccttcg
     2941 gattgttcgc ctagctggaa agtgccgatg gaatcggcga tgcgggcgcg ctcttcgcgc
     3001 gtgagcacgc tgttgggcgg tacgcgcgcc acggcgaggc ggggcacgcg agcttcgaat
     3061 tcacagagcc agtaattcca gtcgaacatc gcggactccc ggttcgtctc gaccccgcat
     3121 cggagtctgg caatgccgga ggccggaatg gtgataagtc aggatgcaat tcgggcagat
     3181 tgtgatccgc ccctcgtccg atcgaagaat ggacgcttga cacgcggact ttaaggccta
     3241 gaatgcgcgc ccctatgcat cctaactacc gcaattcgtt tgcccgcccg gtgttcgccg
     3301 cggcgctgaa acgcttgatt ctcgcgaatc ggcgcagcgc aaccgcgtgc gcgctccgcg
     3361 acgcaggcga aacggacgtt ctgatgcgtt agctcttcgg tcaaatggag agcggcattc
     3421 aggcagctct ccgcaaggac tcacacgagg ccccggtcag ctgccgctga tcggggcctc
     3481 gtcgttttgg cggcgggaac ccggccggaa aacttatttg aggattcggg gtgtggcgaa
     3541 agcgacacct cgtcaggagg tctttgtccc gatttttcgc agcggtccgg tgtctaacca
     3601 ccggctggcg cgctttcacc tggcagcagt cgtgattgcg tgtgccgcga aacagcgagg
     3661 cgataaccac cacaagctag ctcagtgggt agagcgacgg tttattccgt tggccacggg
     3721 ttcgaatccc gtgcttgtaa ccagctgtta actggtttga tggaagtgaa gaagaagcgg
     3781 ggcgtgccct gcatcgaggt gacctggcga tccggcgtgg aagcggatcg cagcgcgccc
     3841 ggcgccatga gttgccggtg agcgcggcgt ccgaagtgca ggacgcttcc aaggttcacg
     3901 cgcgccgtat cggcgcgcgc ggacgtggaa gctgaacgca tggaaggcgc cgcgcagccg
     3961 gcaaaccgct ccgggggtgt gggtatcacc tcgacctgtt ggatgccgcc gcggcaatgg
     4021 ggccgcggcg gtgaggtgga aggaaggaga aggaaaatgc tgggtttgaa gcgattgtcg
     4081 gtggcgcagt acgagcgtgc gctggtgttc cgcgagcgca gcctcgagcg tgtgctcact
     4141 cgcggcgtgt attggctgtg ggatccgatg gcgcgtctca cggtgcaggt ctatgacgca
     4201 accgtgccgg aactcgtgtt gccgcgagcc gacgtgctcg tggccgaggc gcgtgcactg
     4261 ctggaaccgt acgtgcagat cgtggagctc ggcgaccgcg aggtcggcct ggtctacagg
     4321 aacgatcgcc tgacgggcgt tctcgcaccg ggctcgcgtc agttgtactg gcgtggatcg
     4381 gtgagcgttc gcgtcgaggt ccgcgacatc gcgaacgaat acgcgctgga cgcgaacacg
     4441 gcgcgtgtac tgacgcgcgc gaagggcgtg gcgggtgcga gcgacgccat cagcacggtg
     4501 gaggtcccgg acacgtccgt gggtctgttg atcgtcgacg gtgagctgcg cgaggtgttg
     4561 aagccgggtt tgtcggcgta ctggaagtac cagcgcgtgc tgcgcgtgga gctcgtggag
     4621 cttcgcttgc aggccatgga agtgtcgggc caggaaatcc tgacgcgcga caaggtgagc
     4681 ttgcgcgtga acctgaccgc cctgtggcag gtgacggacg ccgtcaaggc gcgtgcgacg
     4741 gtcagcaact tcgtggacta cgtctacaag gagctgcagt tcgcgctgcg tgaggcggtg
     4801 ggtacgcgca cgctggacga gttgttaggc gacaagggtg cgctcgaccg cgaggtgagc
     4861 gcttcggcgg ccaggctcga ggccagtggt cttgcggtgc gctcggtggg tgtgaaggac
     4921 gtgatcctgc cgggcgagat gaagaccatc ctcaaccagg tggtcgaggc ggaaaaggtg
     4981 gcgcaggcca acctgatccg tcgccgcgag gaaacggccg cgactcgttc gctgctgaac
     5041 accgcgcgcc tgatggaaga gaacccgacg ctgttgcgtc tgaaggagct ggagacgctg
     5101 gagaaggtga ccgagaagat cggcaacctg accgtttacg acggcctcga aggtgtattg
     5161 aagaacatgg tgccgcgtct ttcgtagacg cggttaccgt gacaaagttg aatcaaggtg
     5221 attgatatgc agaacgaaca ctatgaaacg atgaatgtcg aaggcggccg ccacgtgaag
     5281 atgtggacgc gcggcgtgcc tgtcgatgag caggcgaagg cgcagctcgc gaacatcgcg
     5341 cgcatgccgt tcgtccactc gcacctggcg gtgatgccgg acgtgcacgt gggcaaaggc
     5401 gcgacggtgg ggtcggtgat cgcaacgcaa ggcgcgatca tcccggcagc ggtcggcgtc
     5461 gatatcggct gcggcatgat ggccgtgcag acttcgctca aggcgagcga tctgccagag
     5521 tcgttgtccg cgctgcgttc gcgcatcgag cgcgccgtgc cgcatggctc atcgaaggtg
     5581 cgtggtcgct acgaccgcgg atcctggggg atcacgccga attcggtgat ctcgcgttgg
     5641 cgtgcgttga gcgagcggtg tgacgccatc acggcgaaat acccgaagct caagcccaag
     5701 gagccgcaga agcagctggg aacgctgggt ggcggcaacc acttcatcga ggtctgtctc
     5761 gacacggacc agacggtgtg ggtgatgttg cactcgggtt cacgcggcat cggcaacatg
     5821 atcggccagc tcttcatcga gctggcacgg caggatatga agaagcattt catcaacctg
     5881 ccggatcgtg atctcgcgta tctcgtcgaa gggaccgaac acttcgacga ttacgtcgag
     5941 gcgatgacct gggcccagga ctacgcggcg gaaaaccggc gcacgatgat ggacgcggtg
     6001 cttcgcgtga tgcgtgagga gctgcgcgcg tttcagatgg gtgaggtggc ggtgaactgc
     6061 catcacaact actgcacgcg tgagctgcat ttcggcgagg agctcatcgt gacccgcaag
     6121 ggcgcggtaa gcgcgaagtc tggcgagctc ggaatcatcc cgggttccat gggtgcgcgt
     6181 tcgtacatcg tgcggggcct ggggaacacc gagagtttcc agagctgcag tcatggcgcg
     6241 ggccgcgtga tgtcacgtac
//
LOCUS       DASQJS010000445         9177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2491252, whole genome shotgun sequence.
ACCESSION   DASQJS010000445 DASQJS010000000
VERSION     DASQJS010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9177
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2491252"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1790)
                     /locus_tag="VEW08_17570"
     CDS             complement(<1..1790)
                     /locus_tag="VEW08_17570"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42604.1"
                     /translation="MNAELKPGGVLDAGFAAPGEVPNPFVGPNPIPEKSELFARDKER
                     GDLVDLLIAERFVLLFGPTGAGKTSLINAGLIPDITARRVRCLPVVKLEATSLDDGVN
                     PYLACLYRRLFAVKDDQALPENLENAFFEQLQKCEGRRVLVIIDTLEQLFTNAAHTDK
                     SAIGFLRILEILHSEYSNLMLLVAMREEFIGRLEGLARPVRPGGRLRLGPLRGDRARE
                     AIMKTAARVKVTLDESCVVPIVRQLAEFAVVETKDDQLVSATDDIAPWHASHGVTRKR
                     SDETALVPIDQLFVEPLYLQLTCFLNWQKLVDIARRFEASPAAAGSAGISVGDTIEAY
                     VESVIVETAKLHASIVSERTLRVFLAERMVASSGELESRREEEARNRFGIERDVLLTL
                     DAKKLIRREPRNGRDEYELSHFLIAKAVRRNYLNWCLTKLHPLQIRARQWARKDEEPP
                     LLRKAELAEMRTWLASHPELENCDDTKFVLASQKALTARKLGWRRIVFMSSIATLAVI
                     ALLGVLGTSQLYRHSDEMHAFENDLNSTIEKLRMLRTDLEDRARIAARFGELGDLVSA
                     RPEAEHAIADIQQFLATVDPDKDPRKSMNFV"
     gene            complement(1787..3913)
                     /locus_tag="VEW08_17575"
     CDS             complement(1787..3913)
                     /locus_tag="VEW08_17575"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYJ42605.1"
                     /translation="MQDLPSLELRLRLQEANRLYVACEYSHPSSEEVQAIEALGGQFS
                     EAKFLALANDINGYGRELANLLFADPRVVAFCAPIFADAMKAQGVVRVRIAITASALP
                     LNALHWEKLLHPTDDVPLARARYVVFSRLTVQSDWRTSGPRRLDSLSSLMCLADDLRG
                     RDGQPPPFPSFGGAAFELAAKTYLEGDNLQSIGAGFTATRLALREGLSSGVDVLYAVC
                     HGVIDKSGEPKLVLHAADGTPDYLSGTEITQMFRELLVVPRLAVLVSCVSAGTGGGDS
                     NSIHIAPRLAEAGVACVIGFNGVIPAQTALDFCRALFENLRADADIEKAVRDARLAMG
                     DDAWWMPVLLTRLRAGALWYRQGFGESSMRVAEAPTEFWEDMVDSVQERQVTPIIGPD
                     VTTSLLGSRRELALKWALANGYPLKPGGNDSLPQIAQFLSTMYKRPKPITKAIKYAYD
                     QLFCAGATTDDLRNADLPAIILAIDKIVQERLAHKWGSQDVLEPYRAIADLDANVYVS
                     LDWTDHLLHALRHRGKDPIVIVPWYWQNSQRRSDDSDSVKTLHEILCENASRKPDVRH
                     PVLYYMFGRLAEDYRSIAMSEDDHFDALIELSRDEYQFPSSVRSALCGSNLMFIGCNL
                     QEWNFRVMLRLVVTRAGFSPSNRDFRHVAANIGPEDSDLSDPQRARKYLAKYLTNSHI
                     DLYSGTATDFMRDLTTNIGRRARDAA"
     gene            4185..6287
                     /locus_tag="VEW08_17580"
     CDS             4185..6287
                     /locus_tag="VEW08_17580"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYJ42606.1"
                     /translation="MASATLKIFALDQAQAGYPAELTEDGKAPVKGLLPAAAFVSGKW
                     TVALNVPGAPAPPARNPGDPAPRDIVETVVRTPGQDRANVDWNSLSAALFEMLFPVGP
                     LRTAYAQLDDTRLFLDIEPAELERVPWELAKSSNPTRRLSLNYTQSRLTAAPRAAARP
                     TSDWPFRILIFVGCSPTDEGKLQVDKEIEAIRRVFIRFGRSVDVRVESRLTLEELSAV
                     IKGGPKQDRWEPHVFHFVGHTGVLPDDSYGLAIDRSETDGASWVWSQESIQQDLDLLE
                     WTPTFVFLNACRTADEPAATWTMQRSFIQAGARCALTMQADVAGEIAGKFAGKFYEQI
                     AAGDTVQEAVAEARGVVYRFNSSTTVIDWGLPSLTTTETETKLFTPRARPPDPGYAKC
                     QEFDEARIFANCEPSRRLLTHWMCPVRAQNKAAESPNVVVLTGPARCGKSHLLKWAME
                     SWVLSGALVRYFELNDGSPRNFLSILRQIRDGDGKGGARYLQRQLPVEAFKRCNWTMK
                     NLLANGQPGEWIEAEHPEAEIPDDPKLQILRANGEDRLEPAICAEFHAALRSLAEKQP
                     VVLVFNKVTDGVTRLVSPDDFAQLVTHLFKPIALEKDGRVKLVFSVSDVERDAYSLRK
                     QDLPQLAYEVPANMDNDALVNYAVEMLRFSNADYVRQLADLLLKMPDEKQRVGMARLG
                     KIRETLNSSYFADIERMQ"
     gene            6284..>9177
                     /locus_tag="VEW08_17585"
     CDS             6284..>9177
                     /locus_tag="VEW08_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42607.1"
                     /translation="MSAAADLEDLLAKLRGLEKGTTLDEALAMLRPPEPAAVIETRRR
                     CALVRAFDKDVYAELTRDLPDAPKLKDFVASNDVRRQGPQRWAFDELTRNALLRAWKL
                     TDTDFRNWNGRLAKFFLGRNRPEERLDAMFHLAASPQPDEGAEYFEKWYAEADAKFDL
                     AHCNALLEILRVREATRGTKLTRAWNDHRQFYQARQFFGDDYFKTGAYFERDEPLKKF
                     EQLLKDGSKQWIYHVHATGGTGKTMFLRWLAARYLVPMHIPCARIDFDDFPLSGVLEY
                     PNRIFTRIIDQLASQVPGGVLTALREKLQRELESPGWNSNVPEEIGRQLRGANLQTNL
                     VVMLDTLEDVTRTAKVWLKTCLQTLRRLHELHPRLVVVLSGRYDISVHTNALLPGEAV
                     VYELMRFSVPEADAYLAKRGIPKGDARTALVERAGENAELDDDKLAGRNPFKLAMFAE
                     LALNKTGLTAEMVRQLPRVDIAYLIQRVIMRISSQPLRWAVRYGVVVRHLNKAVVDAV
                     LLPALKEALRGKTADDPNSALGDFSKVWEPDPALAETVTTDDIWNELKTYVRERGWIS
                     LSGSEADGTEELRFHPDVVTPTRALLRDQTIYRDLQRHAAEFFHANSGLDDAQAPPDA
                     DAAVRNACEAVFHRFQVDGPAARDYWHANLRKIEGWFGLDSAVRYASEICGPAFALAQ
                     VDPMAPFMNAAVLREAHCEAADLLLQTIAHRAIDSGAEFTQFQLHVEWAKAVATKHGL
                     PPVPKFLVAIHDAASAKTGRAIEMLEEARRQALDPRERVFLTLVLARRVREESPQDSA
                     RYLEEALPDLPSARRVWVQAYSIAFELLRLYQALGDHGGVMRTLDTLRGAAGQQRMFL
                     DPVDQAAAGYSLEIGDLETAAACIERLKSSSVPPRRSAVTDLEAQLALERGEVLKVIT
                     LAREGLAGVADQTDSEGLRADFHALQGVAHKHLLAYTDAFDCFERAGVVF"
BASE COUNT         1710 a         2896 c         2920 g         1651 t
ORIGIN      
        1 acgaagttca tgctcttgcg gggatccttg tcgggatcga ccgtggcgag aaattgctga
       61 atgtccgcga tggcgtgttc ggcctcgggt ctcgcggaca ccaggtcacc gagctcgccg
      121 aagcgcgcgg cgatgcgcgc ccgatcctcg aggtccgttc gcagcatcct caacttctcg
      181 atggtggagt tgagatcgtt ctcgaatgcg tgcatctcgt cgctgtggcg atagagctgc
      241 gatgtgccta ggacgccgag taacgcaatg actgcaagag ttgcgatgct gctcatgaag
      301 acgatgcgcc gccagccgag ttttcgcgca gtcagtgctt tctggcttgc aagcacgaac
      361 ttggtgtcat cgcagttctc gagttccgga tggctcgcaa gccacgtgcg catctcggcg
      421 agctcggctt tcctgagcag gggcggctct tcgtctttcc gtgcccattg gcgcgcgcga
      481 atctgcaacg gatggagttt cgtcagacac cagttgaggt agttgcgccg caccgccttg
      541 gcgatcagga aatgggagag ttcgtattca tcgcgcccat tccgcggctc gcggcggatc
      601 agcttctttg cgtcgagcgt gagcagcacg tcacgctcga tgccgaagcg atttcgcgcc
      661 tcctcttctc gcctcgattc cagctctcca ctgcttgcga ccatgcgctc ggcgaggaac
      721 acccgcagcg tacgttcgct cacgatgctc gcgtgcagct tcgcggtttc gacgatgacc
      781 gattccacgt aggcctcgat ggtgtcgccc accgaaatac cggcactgcc ggccgcggca
      841 ggactcgcct cgaagcggcg agctatgtcg accagcttct gccagttgag gaagcacgtg
      901 agctgcaggt acagcggctc gacgaacagc tggtcgatcg ggaccaaagc ggtctcgtcc
      961 gaacgtttgc gcgtgacgcc atggcttgca tgccatgggg cgatgtcgtc ggtggcggaa
     1021 acgagctggt catccttggt ctccactacc gcgaactcgg cgagctgccg cacgatcggc
     1081 acgacacaag actcgtccag cgtcaccttc acacgcgctg cggtcttcat gatggcttcg
     1141 cgcgcgcggt ccccccgcaa tgggccgagc cgcagacgtc cgccgggccg cacgggccgg
     1201 gcgagacctt cgaggcgccc tatgaactcc tcgcgcatgg caaccagcaa catcaggttg
     1261 ctgtactcgg aatgcagtat ctcgagtatg cgcaggaaac caattgccga cttgtcggtg
     1321 tgcgcagcgt tcgtgaagag ctgctcgagc gtgtcgatga tcacgagcac gcggcggccc
     1381 tcgcatttct gcagctgctc gaagaatgcg ttttccagat tttccggcag cgcctgatcg
     1441 tctttgaccg caaacaggcg tcggtacagg cacgcgagat acgggttcac gccatcgtct
     1501 aaagaagtgg cttcgagctt caccaccggc aggcacctga cgcgccgcgc cgtgatgtcc
     1561 gggatgagac ccgcgttgat cagcgatgtc ttgcccgcac ccgtgggacc aaacagcagg
     1621 acgaagcgct cggcgatgag cagatcgacc aggtcaccgc gctccttgtc gcgcgcgaac
     1681 agctcggact tttccggtat cggattcggc ccgacgaatg gattggggac ttcgcccggc
     1741 gccgcgaaac ccgcgtccag gactccccct ggcttcaatt ccgcgttcac gcagcgtctc
     1801 gcgctcgccg gccgatgttg gtagtcaggt cgcgcatgaa gtccgtggcc gtaccggagt
     1861 acaggtcgat atgtgaattg gtgaggtact tggccagata cttgcgcgcg cgctgcggat
     1921 cactgaggtc ggaatcttcc ggaccgatgt tcgcggccac gtggcgaaag tcgcgattcg
     1981 acgggctgaa gccggcgcgc gtcaccacga ggcgcagcat cacgcggaag ttccattcct
     2041 gaaggttgca accgatgaac atcaggtttg atccacacaa cgcgctgcgc acgctggacg
     2101 gaaactggta ttcatcgcgc gacagctcga tcagcgcatc gaagtggtcg tcttccgaca
     2161 tcgcgatgga gcggtagtct tcggcgagcc gtccaaacat gtagtagagg accgggtgac
     2221 ggacatccgg cttgcggctg gcgttctcac aaaggatttc gtgcagcgtt ttcacactgt
     2281 ctgaatcgtc tgagcggcgc tggctgttct gccagtacca cggcacgatg acgatgggat
     2341 ccttgccacg gtgccgcagg gcatgcagaa gatgatcggt ccagtcgaga gacacgtaca
     2401 cgttcgcgtc cagatccgcg attgcacggt acggttcgag tacgtcctgg ctgccccact
     2461 tgtgtgccag ccgttcctgg acgatcttgt cgatcgcgag aatgatggcg ggcaaatccg
     2521 cgtttcgcag atcgtcggtg gtggcgccgg cgcagaagag ctggtcgtag gcgtacttga
     2581 tggccttcgt gatgggcttt ggccgcttgt acatcgtcga caggaactgc gcgatctgtg
     2641 gaaggctgtc gttgccgccc ggcttgagtg gatagccgtt ggccagcgcc cacttgagcg
     2701 cgagttcgcg acgcgagccc aggagcgagg tggtaacgtc cggcccgatg atcggcgtta
     2761 cctggcgttc ctggacgctg tcgaccatgt cttcccagaa ttctgtcggc gcttcggcga
     2821 cacgcatcga ggattcgccg aagccctgcc gataccacag cgccccggcg cgcagcctgg
     2881 tgagcagcac tggcatccac catgcgtcat cgcccatcgc gagccgcgcg tcacgcaccg
     2941 ccttctcgat gtcggcgtcg gcacgcaggt tctcgaacag cgcgcgacag aaatcgagcg
     3001 cggtttgagc cgggatcacg ccattgaacc cgataacgca agcaacaccc gcttcggcca
     3061 ggcgcggcgc gatgtggatc gaattggaat cgccgccgcc ggtgcctgcg ctgacgcagg
     3121 acaccaggac cgcgagccgc ggcaccacca gcagctcgcg gaacatctgc gtgatttccg
     3181 tcccggagag gtagtcaggc gtaccgtctg ccgcgtgcaa taccagcttg ggttcgccgg
     3241 acttgtcgat gactccgtga cacacggcgt agagcacatc gacgccggac gagagtcctt
     3301 cgcgcaaggc caggcgcgtt gccgtaaagc cggcgccgat gctctgcagg ttatctccct
     3361 cgagataagt tttcgccgcc agctcgaatg cggcaccgcc aaagcttggg aacggcggag
     3421 gttggccgtc gcggccgcgc aagtcgtcgg ccaggcacat gaggctgctc agcgagtcga
     3481 gacgccgcgg gcccgaggta cgccagtccg actggacggt gagtcgcgag aagacgacgt
     3541 agcgggcgcg cgccagcggc acgtcgtcgg tgggatgcag cagcttttcc caatgcaatg
     3601 cattcaacgg cagcgccgaa gccgtgattg cgatgcgcac ccgcacaacg ccctgcgcct
     3661 tcatggcgtc ggcaaagatc ggcgcgcaga aagctacgac ccgagggtcc gcaaacagaa
     3721 ggtttgccag ttcgcgccca tagccattga tgtcattggc cagcgcgagg aatttcgcct
     3781 cggagaactg acctccgagc gcctcgatgg cctggacctc ctcgctgctc ggatgggagt
     3841 actcacaggc cacgtacagc cggtttgcct cctgaaggcg caagcgaagc tcgagactgg
     3901 gcaggtcctg catgggttta cctgtctagg caggtttgcg atcgtggtaa tagttgtcca
     3961 ttaactgctt actgtcgcca cgcggtaact tgtctcactt gcacctgaaa ttgtcaccag
     4021 gccgcgtgct tcccgcttcc ggttgcgaga catttgtccg gaatttgccc ggcacagctc
     4081 gggatgctgc accacgagaa tcgcaccggt tcgctgcgct gccgtagaat ccggtggcag
     4141 gaagttaggc cggcagtaac ggaacgagct acccagggga caggatggcg tcggcaacgc
     4201 tgaaaatttt cgcgctcgac caggcacagg ccggttatcc ggccgagctc acggaagacg
     4261 gcaaggcgcc ggtgaagggt ctgctgccag cggccgcgtt cgtcagcggc aaatggaccg
     4321 tggcgctgaa cgtgcccggt gcgcccgcgc cgcccgcccg gaatccaggc gatcccgctc
     4381 cgcgcgacat cgtggaaacg gtagtcagga cgccgggcca ggatcgcgca aacgtggact
     4441 ggaactcgct ctcggccgcg ttgttcgaaa tgttgttccc ggtgggtccc ttacgcaccg
     4501 cctacgcgca gctggacgat acccgcctgt tcctggacat cgaaccagcg gagctcgagc
     4561 gagtgccctg ggagctcgcc aagtcgtcca acccgacgcg acgcctgtcg ctgaattaca
     4621 cgcagagtcg actcacagcg gcgccacgcg ccgccgcgag gccaacctcg gactggccat
     4681 tccgcatcct cattttcgta ggttgttcgc cgacggacga gggaaagctg caggtcgaca
     4741 aggaaatcga ggcaatccgc cgcgtcttca tcaggttcgg gcgctccgtg gacgtgcgcg
     4801 tcgaatcacg cctgaccctg gaagaactat ccgccgtcat caagggaggc cccaagcagg
     4861 accgctggga gccacacgtg ttccacttcg tcgggcacac cggagttctg ccggatgaca
     4921 gctacggcct ggcaatcgac cgctcggaga ccgacggcgc gagctgggtg tggtcgcagg
     4981 aatcgattca gcaggacctc gacctgctcg agtggactcc gacgtttgtc ttcctgaatg
     5041 cctgccggac cgcggacgag ccggccgcga cctggacgat gcaacgcagt ttcattcagg
     5101 ccggtgcccg gtgcgcactc accatgcagg ccgacgtcgc cggcgaaatc gccggcaagt
     5161 tcgccggcaa gttctacgag caaatcgccg cgggcgacac cgtgcaggag gccgttgcgg
     5221 aggcgcgcgg cgtggtctac agattcaact cgagcaccac ggtcatcgac tggggactac
     5281 cgtcgctgac cacgaccgag accgagacca aactattcac accccgtgcc aggccgcccg
     5341 acccggggta cgccaagtgc caggagttcg acgaagcgcg catcttcgcc aactgcgaac
     5401 catcgcggcg cctgctcacg cactggatgt gcccggtgcg cgcgcagaac aaggccgctg
     5461 aatcccccaa cgtcgtcgtg ctcaccggcc cggcacgctg cggaaagtcg cacctgctca
     5521 aatgggcaat ggagtcctgg gttctgagcg gcgcgctggt gcgctacttc gagctcaacg
     5581 acggctcgcc gcgcaacttt ttatccatcc tgcgccagat tcgcgatggt gacggaaaag
     5641 gtggcgcgcg ttacctgcag cggcaactgc ctgtcgaggc attcaagcgc tgcaactgga
     5701 cgatgaagaa cctgctcgcc aatggccagc ccggcgagtg gatagaggcc gagcaccccg
     5761 aggcggaaat ccccgatgat cccaagctgc aaatcctcag ggcgaacggc gaagaccggc
     5821 tcgagcccgc gatctgcgca gagttccatg cggcgttgcg cagcctcgcg gaaaaacagc
     5881 cggttgtact ggtgttcaac aaagtgaccg acggcgtcac caggctcgtg agcccggatg
     5941 atttcgcgca gctcgtcacc catctgttca aacccatcgc gctcgaaaaa gatggccgcg
     6001 tgaagctggt tttcagcgtg agcgacgtcg agcgagatgc gtacagcctg cgcaagcagg
     6061 acctgccgca gctcgcgtac gaagtacccg cgaacatgga caacgacgcg ctggtgaatt
     6121 acgcggtcga gatgctgcgc ttttcgaacg cagactacgt gcggcagctc gcggacctgc
     6181 tgctcaagat gcccgacgag aagcagcgcg tcggcatggc gcggctgggc aagatccgcg
     6241 agacgttgaa cagttcgtac ttcgccgaca tcgagcgcat gcaatgagcg cggcggcgga
     6301 tctcgaggac ttgctcgcca aactacgcgg cctcgagaag ggcaccaccc tcgacgaggc
     6361 gctcgccatg ctccgtccgc ccgagccggc cgcagtcatc gaaacgcggc gtcgttgcgc
     6421 actggtgcgt gcttttgaca aggatgtgta tgccgaactc acgcgtgacc tgccggacgc
     6481 gccgaagctc aaggattttg tcgccagcaa tgacgtccgc aggcagggtc cgcagcgctg
     6541 ggcgttcgac gagctgactc gcaacgcact gttgcgcgcc tggaagctga ccgacacgga
     6601 tttccgcaac tggaacggcc ggcttgcgaa gttcttcctc ggccgcaatc gtcccgagga
     6661 gcgcctcgac gcgatgtttc atctcgccgc ctcaccgcag ccagacgagg gtgccgagta
     6721 cttcgagaag tggtacgccg aagcggacgc gaagttcgac cttgcgcatt gcaatgcgct
     6781 gctcgaaatc ctgcgggtgc gcgaggcgac acgcggcacg aagctgacgc gcgcgtggaa
     6841 cgatcaccgc cagttctacc aggcacgcca gtttttcggt gacgactact tcaagaccgg
     6901 cgcctatttc gaacgcgacg agccgttgaa aaaatttgag cagttgctca aggacgggtc
     6961 gaagcagtgg atctatcacg tccacgccac gggcgggacg ggcaagacga tgttcctgcg
     7021 ctggctggcg gcgcgctacc tcgtaccaat gcacattccc tgcgcgcgca tcgatttcga
     7081 cgattttccg ctgagcggcg tgctcgagta tcccaaccgg attttcaccc gcatcatcga
     7141 tcagctcgca agccaggtgc cgggtggcgt actcacggcg ctgcgcgaga agctgcaacg
     7201 cgaactcgaa tcccccggct ggaactcgaa cgtgcccgag gagatcggcc gtcagctgcg
     7261 cggcgccaac ctgcagacga acctggtcgt catgctcgac acgctcgagg atgtgacgcg
     7321 caccgcgaag gtgtggctca agacctgcct gcagacgttg cgccggctgc acgaactgca
     7381 tcccaggctc gtggtggtgc tgtccggccg ttacgacatt tccgtgcaca cgaacgcgct
     7441 gttgccgggc gaagcggtgg tctacgaact catgcgcttc agcgtgccgg aagcggatgc
     7501 ctatctggcg aagcgcggca tccccaaggg cgacgcgcgc actgccctcg tcgagcgcgc
     7561 cggtgagaac gccgaactcg acgacgacaa gctggccggg cgcaacccgt tcaagctcgc
     7621 catgttcgcc gaactggcgc tcaacaagac gggcctgacg gccgagatgg tgcggcagct
     7681 cccgcgcgtc gacatcgcct atctgatcca gcgcgtcatc atgcgcattt cgtcgcagcc
     7741 attgcgctgg gccgtccgat acggcgtcgt ggtgcgtcat ctcaacaagg cggttgtcga
     7801 tgccgtgttg ctccccgcgc tcaaggaagc attgcggggc aagactgcag acgatcccaa
     7861 ctctgcgctc ggcgacttca gcaaggtgtg ggaacccgac ccagcattgg ccgaaaccgt
     7921 caccacagac gacatctgga acgaactcaa gacctacgtg cgggagcgcg gctggatctc
     7981 gctctcgggt agcgaagcgg atggcacgga agagctgcgt tttcatccgg acgtggtgac
     8041 gccgactcgg gccttgctgc gtgatcagac tatctaccgc gatctgcaac gccacgcggc
     8101 ggagttcttc catgcgaatt cggggctgga cgatgcgcaa gcacccccgg acgcagacgc
     8161 cgccgtgcgc aacgcctgtg aggcggtgtt ccatcgattt caggtggacg ggcctgcggc
     8221 acgggactac tggcatgcaa acctgcgcaa gatcgagggt tggttcggcc tggactcggc
     8281 ggtgcgctac gcgagtgaaa tttgcgggcc ggccttcgcg ctggcgcaag tcgacccgat
     8341 ggcgccgttc atgaacgcag cggtgctgcg tgaagcgcac tgcgaagccg cggatctgtt
     8401 gctgcaaacg atcgcccaca gggcaattga ttccggcgcc gagttcacgc agttccagct
     8461 ccacgtcgag tgggcaaagg ctgtcgccac caaacatggg ctgccgcccg taccgaagtt
     8521 tctcgttgca atccacgacg ccgcctcggc gaaaacaggc cgcgccatcg aaatgctcga
     8581 agaagcgcgc aggcaggcgc tggatccccg cgagcgtgtt ttcctcacgc tggtgctcgc
     8641 acgccgtgtg cgcgaggaga gcccgcaaga ctccgcgcgg tatctcgagg aggcgctgcc
     8701 cgatctcccc agcgcgcgtc gcgtctgggt tcaggcctac tcgatcgctt ttgaactcct
     8761 gcggctctat caagcgctcg gggatcacgg tggcgtcatg cgcacgctcg atacgttgcg
     8821 tggcgccgcc ggtcagcagc gcatgtttct cgatccggtg gaccaggcgg ccgcagggta
     8881 ctcgctcgaa ataggcgatc tggaaacggc cgcggcctgc atcgaacgac tcaagtcaag
     8941 cagcgtgccc ccgcgcaggt ctgcggtcac ggatctggag gcgcagctcg ctctcgagcg
     9001 cggagaggtg ttgaaggtga tcaccctggc acgcgaaggt ctggccggcg tggccgatca
     9061 gaccgactca gagggcttgc gcgcggattt ccatgcgctg cagggggtcg cgcacaaaca
     9121 tctgcttgcg tataccgacg ccttcgactg tttcgagcgc gccggcgtgg tctttga
//
LOCUS       DASQJS010000446        10278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2176140, whole genome shotgun sequence.
ACCESSION   DASQJS010000446 DASQJS010000000
VERSION     DASQJS010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10278
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2176140"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..551
                     /locus_tag="VEW08_17590"
     CDS             87..551
                     /locus_tag="VEW08_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYJ42608.1"
                     /translation="MTKLNVNGNVVEVDVDPETPLLWVLRDSLGLTGTKFGCGMALCG
                     ACTVHLEGQPVRSCQIPVSAIGDKPVTTIEGLSKDRSHPVQKAWIEHDVPQCGYCQSG
                     QIMSASALLETKPRPSDADIDAAMAGNICRCGTYQRIRAAIRRAAEIKAGHA"
     gene            602..2743
                     /locus_tag="VEW08_17595"
     CDS             602..2743
                     /locus_tag="VEW08_17595"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYJ42609.1"
                     /translation="MSANLSSSLSLSRRTFVGGLVIAISLPACKQSPKSSGEQKVIET
                     NAWLRIGTDNSITFLCDRAEMGQGVYTSLPMLIAEELGVELTRIKVEFAPPGDAYINN
                     LIGAQITGGSTSVRDGWEKLRKAGATARMMLVSAAADDWGVDARNCKVANGVILSPQF
                     KKKTFGEVAEAASKLPVPKDVPLKPAAQFTMIGKAQRRLDTPAKVDGSAIYGIDVKLP
                     GMLYAALAQPPTLGGSVKTFNDDKARAMPGVVAVVLTSSGVAVAADSWWRARKARDAL
                     TIEWDAGPNAALNDAKITQMLRKGAAGPGRVARHDGDAAAAIRSAARVVRADYELPLL
                     AHATLEPQNCTADVRADGADIYVPTQVQQIAQATAAKAAGLDPKAVKVHTTFLGGGFG
                     RRLEVDFIPAAVEVSKALKKPVKLVWTREDDMTHDAYRPPAFDQATGAFDEKGKLIAW
                     NLHLTGPSITARMFPSVTEKAVDPFAVEAAANYPYDVPNVHVDFQQQEIGINVGYWRS
                     VSHSLNCFVAESFMDELAVAVKKNSYEFRKALLEKQPRFLKVLEMVVKESRFGYPPRG
                     HFHGLAVMEGYGTYMAQVAEISLDTGPNAGKIKVHRIYCAADCGQQVNPDTVVAQIES
                     SVIFGLSALMWGEINIQNGRVQQTNFDSYRVARMTDAPRIDSYVLDSGEAPGGIGEPA
                     TALVAPAVCNAIYTATGRRLRSLPLSRHRLA"
     gene            2753..4054
                     /gene="gabT"
                     /locus_tag="VEW08_17600"
     CDS             2753..4054
                     /gene="gabT"
                     /locus_tag="VEW08_17600"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HYJ42610.1"
                     /translation="MATNADLHKRRMAAIPRGVGNSLAVYAERAQNAELWDVEGRRYV
                     DFASGIAVVNTGHVHPHVKAAMAAQLEKFTHGCFQVTPYESYIALAEALNKLAPGPTP
                     KKTIFLSTGAEAVENAIKIARYATKRSAIIAFSGGFHGRTLGCIALTGKVQPYKAGFG
                     PMLPEVFHVPFPMAYHGVTPEDSFAAIDHLFKADVDPARVAAIIIEPVQGEGGFYIAP
                     PEFLRGLRALCDKHGILLIADEIQTGFARTGKTFAIEHSGVEPDLMTVAKSIAGGVPL
                     SAVVGKAEIMDAPVVGGLGGTFAGSPLACAAGLAVLEVLEKEKLNQRAEAMGAKLIAR
                     LKSLQARFSCIGEVRGLGMMVAMELVKNRRADAPDADLTKALVQAAGRRGLILLSCGI
                     YSNVIRILAPLTIPEAQLEEGLGLLEQALAEATQGTASAVA"
     gene            complement(4123..4632)
                     /locus_tag="VEW08_17605"
     CDS             complement(4123..4632)
                     /locus_tag="VEW08_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42611.1"
                     /translation="MKMISLGSISLGLAAALLATTAFAHGDHDPDQGKQKPPPFAILQ
                     LDQVRDATARFIDVEKAEAAGYRDIGLFVPNMGWHYMNDALVDARFDPTKPELLVYAD
                     DPCGGKRKLVAVEYAVPLAESKRAPLGFVGRADEWDANQQFQLWTLHAWLFEFNQDGV
                     FAPFNPRVP"
     gene            complement(4805..5104)
                     /locus_tag="VEW08_17610"
     CDS             complement(4805..5104)
                     /locus_tag="VEW08_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42612.1"
                     /translation="MEPIADTFDAAFSKTVDLANKMADKDKDADLWDIADGMLAGAIQ
                     YWLYSRQPCGDTRCQDCMPISTAEGRMSELKRLIEQLASESEYFHTPTDSNAGRA"
     gene            complement(5197..6084)
                     /locus_tag="VEW08_17615"
     CDS             complement(5197..6084)
                     /locus_tag="VEW08_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42613.1"
                     /translation="MLILFVLAGLLLFVPLPVSPTYAGRTIENAGHTPLFFLVTLGVL
                     FTMRDHPRFQGASLYALAGMIGAGTGFLSEVIQKPLARDASWEDVFADAVGAVLALAV
                     YALFERRTKLRVWQRLGALAVALSCIAIFLAPIVRMTRAYVHRNGQVPVIADFHSRIE
                     LYWTLSFGVNREIVDDALEVEFVADEFPGVSFHEPVPDWRSFRTLIIDVENPASERLN
                     LGVRVHDRRHKHAFGDRFNRRFELAAGERRTLRIPLEDIRQGPRRRLMDMAHISDITL
                     FRGADSGSRQLRVYSLRLE"
     gene            6125..6811
                     /locus_tag="VEW08_17620"
     CDS             6125..6811
                     /locus_tag="VEW08_17620"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HYJ42614.1"
                     /translation="MNIRLSAIEARIIGALIEKQITTPDQYPLSLNALINACNQKSNR
                     EPSMQLDEPTVKFVVEGLGRRHMVVEKSGFGSRVPKYQQIFCNTEFGSLKFTPQETAI
                     VCELLLRGPQTPGELRSRAPRMADFPDPGIVESLLEGLANRAGGPIVAQLAREPNRRD
                     SRWIQLFEELPESARLATAGEVAANKASDAAVVRAPSNAELVARVAMLEGEIAALRAE
                     LAALRQERIA"
     gene            6814..7158
                     /locus_tag="VEW08_17625"
     CDS             6814..7158
                     /locus_tag="VEW08_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004697497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HYJ42615.1"
                     /translation="MPAWAQSAGLLIISNVFMTFAWYAHLKNMAAKPWVIAALASWGI
                     ALFEYLVQVPANRIGYTTLSLGQLKIMQEVITLAVFVPFAILYMKQPLKLDFLWAGLC
                     LVGAAYFAFRGS"
     gene            complement(7155..8174)
                     /locus_tag="VEW08_17630"
     CDS             complement(7155..8174)
                     /locus_tag="VEW08_17630"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HYJ42616.1"
                     /translation="MRYFKSDNTAAVSEEILRAIHEANTGVALAYGADRWSESLDAVY
                     GQFFGTKVRVFAVSSGTAANSLALATLCPPWGTVLTHTEAHIERDECGAPEFFTGGAK
                     LSLVGGAGAKITAADFAERLNWFEPHVHGVQPRMLSITQATERGAVYSPQEISALSKI
                     AHDRGMAVHMDGARFANALVAQKVSPAELTWKAGVDVLSFGVIKNGGMNAEAVVFFDP
                     TRVADFEFRRKRAGQLFCKGRYAAVQLLAYLESGMWQRNAARANRLAARIADAAPALL
                     SEPIATNQVFMRLGPARIEELSREFGFYPWGPAGSGEARFVCSWDTPDEDVDVLCAAL
                     ARFAS"
     gene            8234..>10278
                     /locus_tag="VEW08_17635"
     CDS             8234..>10278
                     /locus_tag="VEW08_17635"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ42617.1"
                     /translation="MKDLSRFRALTLRAAVAAALGLAAPQLMAQDAPAAGDEQNIDEV
                     VVTGTRVATRTRLDTLAPVDVLPQETLTSTGSTELAEALATAAPSLNFPRPSITDGTD
                     HIRPATLRGLAPDQTLVLVNSKRRHQSALVNVNGSVGRGSAAVDLNAIPLAALETVEV
                     LRDGASAQYGSDAIAGVINLRLRDAREGGNASITYGEYNTEVETERGQRHEDDGPTLT
                     ASGWVGLPLGPEGFLTLSGEYRDRDPTSRGDFDIRVPGSPVTSRYGDANVEDITAYVN
                     AGVPLEGDWNIYGWAGYQQREGDSAAFPRLFNDIIRNVPAIYPNGFLPLITTDIDDLA
                     LGWGVRGKMGSWDADVSLVYGSNEVVYGVEDSLNASYGAASQTSFDAGSMKYDQIVFN
                     AGIVRTFEVGSLPEGVNVAFGIEARTESYQIEAGEPTSYNRGPVINGAVGAQGFPGFQ
                     PSNELDEDREAYSVYADIESRLTEKFLASVAVRAEDYSDFGSAVTGKIAGRFDFTDSF
                     ALRGAVSTGFRAPGLQQEFFTSTATNFINGEPFEVGTFPATSEVAITLGAQPLDAEES
                     VNYSLGTVFRFGGFEATIDAYRIDIDDRIVLSENLQSPQVTALLDPFDVTVARFFING
                     VDTKTEGIDVVTRYLWRTESLGKFEFVAAGNWNDTEVEKVPSTNVIDNICVGQPASCV
                     "
BASE COUNT         1958 a         3321 c         3243 g         1756 t
ORIGIN      
        1 cacctgtcca ggaagctggc cccctcctac aaacaacgcg accggcatat agtcgccaca
       61 gtcgccgaaa accaggggat tcgctcgtga ccaagctcaa cgtcaatggc aacgtggtcg
      121 aagtggacgt cgatccggaa accccgctgc tgtgggttct gcgcgactcg ctgggcctga
      181 cggggaccaa gttcggatgc ggcatggccc tgtgcggcgc ctgcaccgtg catctcgagg
      241 gccagccggt gcgctcctgc cagatcccgg tcagcgccat tggcgacaag ccggtgacga
      301 ccatcgaggg actctcgaag gatcgcagcc acccggtaca gaaggcgtgg atcgaacacg
      361 acgtgccgca atgtggctac tgccagtccg gccagatcat gtcggcttct gcgctgctgg
      421 aaaccaagcc gcgaccctcc gacgccgata tcgacgccgc catggccggc aacatctgcc
      481 ggtgtggaac ctaccagcga atccgcgcgg ccatccgccg tgccgcggag atcaaagccg
      541 gccacgcctg atcgtcggga cgcttcgaca acgacaacga gtttccgggg aggaaaccag
      601 catgtccgcg aatctatcgt catcgttatc cttgtcacgc cgcacgttcg tgggcggcct
      661 ggtcatcgcc atcagcttgc cggcctgcaa gcagtcgccg aaatccagcg gcgaacagaa
      721 ggtcatcgag accaacgcct ggctgcgcat cggcaccgac aacagcatca ccttcctctg
      781 cgaccgcgcg gaaatgggcc agggcgtata cacctcgctt cccatgctca tcgccgaaga
      841 actgggcgtc gaactcacgc gcatcaaggt cgagttcgcc ccgccgggcg atgcatacat
      901 caacaatctc atcggcgcgc agatcaccgg cggcagcacc agcgtgcgcg acggctggga
      961 aaaactgcgc aaggccggcg ccacggcgcg gatgatgctc gtcagcgctg ccgcggatga
     1021 ttggggcgtc gacgctcgaa actgcaaggt cgccaacggc gtcattcttt ctccgcaatt
     1081 caagaagaag acgttcggcg aagtggccga ggccgcctcc aaattacccg tgcccaagga
     1141 tgtgccgctc aagcccgcgg cgcagttcac gatgatcggc aaggcgcaaa gacgactgga
     1201 cacgccggcc aaggtggacg gcagcgccat ttacggcatc gacgtgaagc tgcccggcat
     1261 gctgtacgcg gcgctggcgc agccgccaac gctcggcgga tcggtgaaga ccttcaacga
     1321 tgacaaggcg cgcgccatgc cgggcgtggt ggccgtggta ctcacgtctt cgggagtcgc
     1381 cgtggccgcg gattcgtggt ggcgggcacg caaggctcgt gatgcactca ccatcgaatg
     1441 ggatgcgggc ccgaacgccg cgctcaacga tgcaaagatt acgcagatgc tgcgcaaggg
     1501 ggccgcgggc cccggacgtg tggcacgtca tgatggtgac gcggccgcgg cgatcaggag
     1561 tgcggctcgc gtggtgcgcg ccgactatga actgccgctg ctcgcccacg ccacgctcga
     1621 gccgcagaac tgcacggccg acgtgcgcgc cgacggcgcc gatatctacg tccccacgca
     1681 ggtccagcag attgcccagg ccacggccgc caaggccgcc ggcctcgatc cgaaggcagt
     1741 gaaggtccac accacgttcc tgggcggagg tttcggccga cgcctggaag tggatttcat
     1801 tcccgcggcc gtcgaggttt cgaaggccct caagaagccg gtgaaactcg tgtggacgcg
     1861 cgaagacgac atgacccacg acgcctatcg gccgcccgct ttcgaccagg ccaccggcgc
     1921 gttcgacgag aagggcaagt tgatcgcctg gaacctgcac ctgacgggac cctcgatcac
     1981 cgcgcgcatg tttccgtccg tcaccgaaaa ggcggtggat ccgttcgcgg tcgaggcggc
     2041 cgctaactac ccttacgacg tgccgaacgt gcacgtggat ttccagcagc aggaaatcgg
     2101 catcaacgtg ggctactggc gctcggtgag ccattcgctc aactgtttcg tcgccgagag
     2161 cttcatggac gagctggccg tggccgtcaa aaagaattcg tacgaattcc gcaaggcgct
     2221 gctcgagaaa cagccgcgct tcctcaaagt gctggaaatg gtggtcaagg aatcgcgctt
     2281 cggatatccg ccgcgcgggc acttccacgg actcgcggtc atggagggct acggcactta
     2341 catggcgcag gtggcggaga tctcgctcga cacggggccg aatgccggca agatcaaggt
     2401 gcatcgcatt tattgcgccg cggactgcgg ccagcaggtg aatccggaca cggtggtcgc
     2461 gcagatcgaa agctccgtca tcttcgggct ctcggcactc atgtggggcg agatcaacat
     2521 ccagaacggc cgggtgcagc agacgaattt cgacagctac agggtggcac gcatgaccga
     2581 tgcgcccagg atcgattcct acgtgctcga cagcggcgag gcgccgggtg gcatcggcga
     2641 gccggcaact gcactggtgg cgccggccgt ctgtaatgca atttacacgg ccaccgggcg
     2701 tagacttcgg tcactcccct tgagcaggca ccgattagcc tagagcagac ctatggcaac
     2761 aaatgctgat ctacacaagc ggcgcatggc cgccattccg cgtggcgtgg gcaactcgct
     2821 cgcggtgtac gccgagcgcg cgcagaacgc cgagttgtgg gatgtcgaag gccgccgcta
     2881 cgtggacttc gccagcggca tcgccgtggt caacacaggc catgtgcatc cgcatgtgaa
     2941 ggccgcgatg gccgcgcagc tcgaaaagtt tacgcatggt tgcttccagg tcacgccgta
     3001 cgaaagttac atcgcgctgg cggaagcgtt gaacaaactt gcaccaggtc cgacgccgaa
     3061 gaaaaccatt tttctgtcca ccggcgccga agccgtggag aacgcgatca agatcgcgcg
     3121 ttacgccacg aagcgctccg caatcattgc cttcagcggc ggctttcacg ggcgcacgct
     3181 cggctgcatc gcgctcaccg gcaaggtgca gccatacaag gcgggcttcg gcccgatgct
     3241 gcccgaagtg ttccatgtgc ccttccccat ggcctatcac ggggtgacgc cggaagattc
     3301 gttcgcggcc atcgatcacc ttttcaaggc ggatgtggat ccggcgcgcg tcgccgccat
     3361 catcatcgag cccgtacagg gcgaaggtgg cttctacatc gcaccgccgg agttcctgcg
     3421 cggcttgcgc gcgttgtgcg acaaacacgg catcctgctc atcgccgatg aaatccagac
     3481 cggcttcgca cgcaccggca agacttttgc catcgaacat tccggcgtcg agcccgacct
     3541 catgacggtc gccaagagca ttgctggcgg cgtgccgctg tcggcggtcg ttggcaaagc
     3601 ggaaatcatg gatgcgccgg tggtcggcgg actgggcggc acgttcgcgg gttcgccgct
     3661 ggcctgcgcc gccgggctgg ccgtgctcga agtgctcgag aaggaaaaat tgaatcagcg
     3721 cgcagaggcg atgggggcaa agctcatcgc acggctgaaa tccctgcagg ccaggttcag
     3781 ctgcatcggc gaggtgcgtg ggctcggcat gatggtggcc atggagctgg tgaagaatcg
     3841 ccgcgcggat gcgccggatg ccgaccttac gaaggctctc gtgcaggccg ccggacggcg
     3901 cgggttgatc ctgctgtcct gcggcatcta ctcgaacgtc atccgcattc tcgcgccgct
     3961 cacgattccc gaagcgcagc ttgaagaagg attgggattg ctggagcaag cgctggctga
     4021 agcgacccag ggcaccgcgt cagccgttgc gtaatgggtg ggtgcggggt gaggaaagtg
     4081 gcgagtcgca ctccccggtt tccccacccg gcacctctcg acctacggta cccgcggatt
     4141 gaagggcgcg aatacgccgt cctggttgaa ctcgaacagc caggcgtgca gcgtccataa
     4201 ctggaactgc tgattcgcgt cccattcatc ggcacgcccc acgaatccga gtggcgctcg
     4261 cttcgactcc gccagcggca ctgcgtattc caccgccacc agcttgcgct tgccgccgca
     4321 cggatcgtcg gcatacacca gcaactccgg cttcgtcgga tcgaacctgg cgtcgaccag
     4381 cgcgtcgttc atgtagtgcc agcccatgtt cggcacgaac aggccgatgt cgcgataacc
     4441 cgcggcttcc gccttctcca cgtcgatgaa gcgcgcggtg gcatcgcgca cctggtcgag
     4501 ctgcaggatg gcgaacggcg gcggcttctg cttgccctgg tccgggtcgt gatcgccgtg
     4561 agcgaaggcc gtggtggcca gcagggctgc ggcgagcccc agggaaattg atcccagtga
     4621 aatcatcttc attttcatct cctgatagtt agtcttctga tcccccggtt gccgggaaag
     4681 actaaccact caggaatgcg gcgcgaatga ccggaagtcc ggtgtcgatg cctcgccgtc
     4741 cgcgcacatg cccaggcgtc cggcattgtt gcccgaacgt acctggaccg gatcttgaga
     4801 tgccctacgc gcgacccgcg ttcgaatccg tgggcgtgtg gaagtactcg ctttcggagg
     4861 cgagctgttc gatgaggcgc ttcagctccg acatgcggcc ttcagcggtg ctgatgggca
     4921 tgcagtcctg gcaacgcgta tccccgcaag gctggcgcga atacagccag tactgaatcg
     4981 ccccggcgag catgccatcg gcgatgtccc agaggtccgc gtccttgtcc ttgtccgcca
     5041 tcttgttggc gagatccacg gtcttggaga aggcggcgtc aaaggtgtcg gcgatcggtt
     5101 ccatggggcg gagtataggc atcgtgcccc ttgcgcgaaa agcctcaatt ggccccggag
     5161 cgacctgcaa gagcagccta tgttcaacgc agtgcactac tccagccgca aagagtaaac
     5221 acgcaattgc cgagagcccg aatcagcgcc gcgaaacagt gtgatgtccg agatatgcgc
     5281 catgtccatg agccgccgtc gaggcccttg ccggatgtct tccagtggta ttcgcaatgt
     5341 gcgccgctcc cccgccgcca gctcgaagcg ccgattgaat cggtcgccga aggcgtgctt
     5401 gtgacggcgg tcgtgcacgc gcacaccgag attgagccgc tcgcttgccg gattctccac
     5461 gtcgatgatc agggtcctga agctccgcca gtccggcacg ggttcgtgga aggagacgcc
     5521 gggaaattcg tccgcgacga attccacctc tagcgcgtcg tcgacgatct cgcgattgac
     5581 gccaaagctc agtgtccagt aaagctcgat gcgcgagtga aagtcggcga tgaccggaac
     5641 ctgaccgttg cgatgcacat aggcgcgcgt catcctaaca atcggcgcga ggaatattgc
     5701 gatgcacgag agcgcaaccg ccagggcgcc gagccgctgc cagacgcgca gcttcgtgcg
     5761 gcgctcgaaa agcgcataga cggcgagcgc aagaaccgca ccgacggcgt cggcgaacac
     5821 gtcttcccag gatgcgtcgc gtgccagcgg cttctggatg acctcactca ggaatccggt
     5881 gccggcgccg atcattccgg cgagcgcata gaggctcgct ccctggaagc gcggatggtc
     5941 gcgcatcgtg aagagcacgc cgagtgtcac caggaagaac agtggtgtgt gcccggcgtt
     6001 ctcgatggtt cggccggcgt aggtcggcga gacgggcagc ggcacgaata gcagcaggcc
     6061 cgccagcacg aacaggatga gcatgtaaag catacggcgg ggcacagccc agtatacttc
     6121 gcgcatgaat atccgcctct cggctatcga ggctcgcatc atcggcgcgc tcatcgaaaa
     6181 gcagatcacc acgcccgacc agtacccgct ctcgttgaat gcgctcataa acgcgtgcaa
     6241 tcagaagagc aaccgggaac cttccatgca gttggatgaa cctaccgtga agttcgtcgt
     6301 cgagggactc ggacgacgtc acatggtcgt ggagaaatca ggatttggca gccgcgttcc
     6361 caagtaccaa cagatcttct gcaataccga gttcggcagc ctcaagttca ctccgcagga
     6421 aaccgcgatc gtgtgcgaac tgctgttgcg cgggccgcag acgccgggtg agctgcgcag
     6481 tcgtgcgccg cgcatggctg atttccccga tcccggcatc gtcgaatcgc tgctcgaggg
     6541 cctcgcgaac cgtgccggtg gccccatcgt tgcgcagctc gcgcgcgaac ccaaccgccg
     6601 cgactcgcgc tggatacagt tgttcgaaga gctgccggaa tcggccaggc tggccaccgc
     6661 cggcgaagtc gcggccaaca aggcgagcga tgccgccgtg gtgcgcgcgc cgagcaatgc
     6721 ggagctggtg gcgcgcgtgg cgatgctgga aggcgaaatc gccgcgcttc gcgccgagct
     6781 cgccgcgctc agacaggaac gcatcgcgtg aatctgcccg cctgggcgca aagcgcgggc
     6841 ctgctgatca tctcgaacgt gttcatgacg ttcgcgtggt acgcgcacct caagaacatg
     6901 gccgccaagc cctgggtcat cgccgcgctc gcgagctggg gcatcgcgct gttcgagtac
     6961 ctggtgcagg ttcccgcgaa ccgcatcggc tacaccacgc tgtcgctggg ccagctcaag
     7021 atcatgcagg aagtgattac gctcgcggtg ttcgtgccgt ttgcaatcct ctacatgaaa
     7081 cagccgctga agctcgattt cctgtgggcc gggctgtgcc tggtaggcgc ggcctacttc
     7141 gccttccgcg gcagctaact agcgaacctc gccagggccg cacacaacac atccacgtct
     7201 tcgtcgggcg tatcccacga acacacgaag cgcgcttcac ccgagccggc tgggccccag
     7261 ggatagaagc cgaactccct ggacaattcc tctatgcgcg ctggcccgag ccgcatgaac
     7321 acctggttgg tggcaattgg ctcactgagc agcgcaggcg ccgcgtccgc aattcgggcc
     7381 gcgagccggt tcgctctcgc ggcgttgcgc tgccacatgc cactttcgag gtaggcgaga
     7441 agttgcaccg cggcgtatcg tcccttgcag aacagttgcc cggcgcgctt gcggcggaac
     7501 tcgaagtccg caacgcgggt cggatcgaaa aacaccacgg cctcagcatt catgccgcca
     7561 ttcttgatga cgccgaacga caacacgtcg acgcccgctt tccaggtgag ctcggcgggc
     7621 gacacctttt gcgccaccag tgcgttggca aagcgcgcgc cgtccatgtg caccgccatg
     7681 ccgcggtcat gagcaatctt cgaaagcgcg ctgatttcct gcggcgagta gacggcgccg
     7741 cgttcagtgg cctgggtaat cgacaacatg cgcggctgca cgccgtgcac gtgcggttcg
     7801 aaccagttga gacgctcggc gaaatcggcg gccgtgatct tcgcgcccgc ccctcccacg
     7861 agcgagagtt tggcgccgcc ggtgaagaat tccggcgcgc cacattcgtc gcgctcgatg
     7921 tgcgcctcgg tgtgcgtgag cacggttccc cacggcggac agagcgtggc gagcgccagg
     7981 gagttggccg cggtacccga agacacggcg aacacgcgta ccttggtgcc gaagaattgc
     8041 ccgtacacgg cgtcgaggga ttcggaccag cggtcggctc cataggcgag tgcgaccccc
     8101 gtgttcgctt cgtgaatggc gcgaagaatt tcctcgctga ccgcggcagt gttgtcagat
     8161 ttgaagtagc gcacgggcgg gcattctacc gtggcgctgc aacgttcccg aattattcaa
     8221 gaaaggagtc accatgaaag atctcagccg tttccgcgca ctcaccctgc gcgcggccgt
     8281 tgcggcggca cttgggttag ccgctcccca actcatggcg caggatgcac ctgccgccgg
     8341 tgacgagcag aacatcgacg aagtcgtcgt caccggcacg cgcgtcgcca ctcgcacgcg
     8401 cctggatacg ctcgcgccgg tcgacgtgct gccgcaggaa acgctcactt ccacgggcag
     8461 caccgagctc gccgaagcgc tcgccaccgc tgcgccgtcc ctgaattttc cgcggccgtc
     8521 gatcaccgat ggcaccgacc acatccggcc cgccactctg cgcggcctgg cgcccgatca
     8581 gaccttggtg ctggtgaatt ccaaacgacg gcaccagtcc gcgctggtca acgtgaacgg
     8641 ctcggtggga cgcggctcgg ccgcggtgga cctcaacgcc attcccctgg ccgccctcga
     8701 aaccgtcgaa gtgctgcgcg acggcgcttc cgcccaatac ggctcggatg ccattgccgg
     8761 cgtcatcaac ctgcgcctgc gcgacgcgcg tgaaggcggc aatgcctcga tcacctacgg
     8821 cgagtacaac accgaggtcg agaccgagcg aggccagcgc cacgaagacg atggtcccac
     8881 tctcacggcg tcgggttggg tcgggttgcc tcttgggcct gaaggattcc tcacgctgtc
     8941 gggcgaatat cgcgaccgtg atcccacgag ccgcggcgac ttcgacatcc gtgtgccagg
     9001 ttcacccgtg acctcgcgtt atggcgacgc gaacgtcgag gacatcaccg cctacgtcaa
     9061 tgccggggtg ccgctcgaag gtgactggaa catctacggc tgggcgggat accagcagcg
     9121 cgagggcgac tcggccgcgt tcccgcgcct cttcaacgac atcatccgca acgtgccggc
     9181 tatctacccg aacggcttcc tgccactcat caccaccgac atcgacgacc tggccctggg
     9241 ctggggcgtg cgcggcaaga tgggaagctg ggatgcggac gtgagcctgg tctatggctc
     9301 caacgaagtg gtctacggtg tcgaggacag cctcaacgct tcctatggcg ccgcttcgca
     9361 gaccagcttc gatgcgggct cgatgaaata cgaccagatc gtcttcaacg ccggcatcgt
     9421 gcgcacgttc gaggtgggca gcctgcccga gggtgtcaac gtggcattcg gcatcgaagc
     9481 gcgaaccgag agctaccaga tcgaagcggg tgaacccact tcctacaatc gcggacccgt
     9541 gatcaacggg gccgtcggcg cgcaaggatt cccgggattc cagccaagca acgaactcga
     9601 cgaggaccgc gaggcataca gcgtctatgc ggatatcgaa tccaggctca cggagaaatt
     9661 cctggcatcg gtagcggtgc gcgccgaaga ctactcggac ttcggctccg cggtcaccgg
     9721 caagatcgcc ggacgtttcg acttcaccga cagcttcgcg ctgcgcggcg ccgtgtccac
     9781 ggggtttcgc gcaccgggac tgcagcagga attcttcaca tccaccgcga ccaacttcat
     9841 caacggcgag ccattcgagg tgggcacgtt cccggccact tcggaagtcg cgatcacgct
     9901 cggcgcgcag ccgctcgacg ccgaggagtc cgtcaactac tcgctgggca cggtgttccg
     9961 cttcggcggc ttcgaagcca ccatcgacgc gtatcgcatc gacatcgacg accgcatcgt
    10021 gttgtcggag aatctgcagt caccgcaagt cacggcgctc ctcgacccct tcgacgtgac
    10081 cgtggcccgg ttcttcatca atggcgtgga cacgaagaca gaaggtatcg acgtggtgac
    10141 gcgctatctg tggcgcacgg aaagcctcgg caaattcgag ttcgtcgccg ccggcaactg
    10201 gaacgacacc gaagtggaaa aggtgccgtc aacgaatgtc atcgacaaca tttgtgtggg
    10261 ccagcccgcg tcttgcgt
//
LOCUS       DASQJS010000447         7849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2492189, whole genome shotgun sequence.
ACCESSION   DASQJS010000447 DASQJS010000000
VERSION     DASQJS010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2492189"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1304
                     /gene="metB"
                     /locus_tag="VEW08_17640"
     CDS             147..1304
                     /gene="metB"
                     /locus_tag="VEW08_17640"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HYJ42618.1"
                     /translation="MTTSLTADTRAVRAGLENCDVTGAVVPPLHLSSTYAFRAFGEKR
                     KYDYSRSGNPTRDLLADALNDLEGGAGAVITGSGMGAITLVGHILPVGARVIAPHDCY
                     GGTFRLFTAWQKRGELAVEFVDFGNKAAVDAALSKPAALVWIETPSNPLLRITDVADI
                     AKRGHAIGALVVADNTFLSPGWQQPIALGADIVVHSTTKYINGHSDVVGGAVIAAKKE
                     VAEQLAWWGNCLGLTGGAFDSYLTLRGLRTLHVRLREHGRNAQAVAEFLAKQKSVKRV
                     YFPGLPSHPGHDIAKRQQSGFGAIVTIEVQGGVDGAKQFSEALELFSLAESLGGVESL
                     IAHPATMTHAAMAPEARAAAGLVDGLLRLSIGIESTEDLLRDLQNGIAALG"
     gene            complement(1305..2198)
                     /locus_tag="VEW08_17645"
     CDS             complement(1305..2198)
                     /locus_tag="VEW08_17645"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ42619.1"
                     /translation="MEAASRRDFLVAGAAATVPTISTAASGKKPAPATRAGLLAGKVA
                     VIYGAAGAMGSAVARAFAREGAHVELAGRTLEKVQALATEIARAGDSAAAAKVDALDE
                     DSIAKHLDAVIGSHGRVDVSFNLISLAGPQGDSLAGMSVEDVVAPVEGAVRTHFLTAT
                     AAARHMARRHSGVILALTAQVARKPYTGSGGFGVACAAIEGLCRQLAVDLGGDGIRVV
                     ALRSSGSPDAPGVAFAIGEHARLAGVTREAFEARIAEKTMLKRMPKMAEVANAAVLMA
                     SDRAGAITAAVTNVTCGEIAD"
     gene            complement(2204..2962)
                     /locus_tag="VEW08_17650"
     CDS             complement(2204..2962)
                     /locus_tag="VEW08_17650"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HYJ42620.1"
                     /translation="MNRIVNREKWIAERKQLLLEEKEFTRRRDALAEKRRALPWVKVD
                     KRYEFDSPNGRVSFADLFRGRSQLFVQHVMQGPGQPLQCVGCALGVDHLESLLPHLEN
                     HDVSVVAIARATMPELEALRTKMQWRTPFYSSFGSDFNYDYGVSFRPEDLAAQRVFYN
                     YTYCDPGIEDISGNSVFYRDASGDIFHTYSNYARGGEDFLGIYRVLEVMPLGRNENGP
                     YHSLTDWVRPHNMYGKGGEVAPTGRYTPPVSCCM"
     gene            complement(2972..3604)
                     /locus_tag="VEW08_17655"
     CDS             complement(2972..3604)
                     /locus_tag="VEW08_17655"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYJ42621.1"
                     /translation="MAETPENSRTRLLAATIDLVRANGYAATRVEDVCAAAGVTKGSF
                     FHHFASKEDLAIAAAGQWNDRAFQVFALASYQRLADPLDRLLGYVAFRKELLSGDIWE
                     WTCYAGTTIQEVHETYPAIRDACAASIEKHLFFLRAIIDDVVREQAVKGVDSESLAVH
                     MQAVIQGAFIMAKARQDSRAAIDSLDHLYRYVELLFGRSTSSGKGKKNQP"
     gene            complement(3671..4732)
                     /gene="leuB"
                     /locus_tag="VEW08_17660"
     CDS             complement(3671..4732)
                     /gene="leuB"
                     /locus_tag="VEW08_17660"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HYJ42622.1"
                     /translation="MKAKIAVIAGDGIGPEVVAEGVRVLEQIGKRFGHEFILEEAPFG
                     GIAIDLTGDPLPQTTLDTCLAANAVLLGAIGGPKWGPSAAVRPEQGLLRLRRELGVYA
                     NLRPIKLHPALRDASVLKPEILDGVDLVFVRELTGGIYFGAKTRTPTLATDLCSYSAS
                     EIERITRVAGRLAMQRRKKVVSIDKSNVLETSRLWREVASHVIHSEFPEVDLEHMLVD
                     SAAMHLLKRPREFDVMLTENMFGDILTDEGAMLASSLGVLPSASIGDGKRGLYEPIHG
                     SAPDIAGKGIANPIGTILSVALLLRHSLELEREAATVEQAVDRCIANGARTADIAEHG
                     RPAMTTRRMGDAVVAALQS"
     gene            complement(4729..5301)
                     /gene="leuD"
                     /locus_tag="VEW08_17665"
     CDS             complement(4729..5301)
                     /gene="leuD"
                     /locus_tag="VEW08_17665"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HYJ42623.1"
                     /translation="MAVKNIRSRTVALPATNVDTDQIIPARFLTSTSKAGFGKHLFAD
                     WRYDAAGNPKPEFVLNRPEAKGAAILVAGRNIGCGSSREHAPWALQDFGFQAVVSTEF
                     ADIFRTNCLKNGLLPVLVDEQTHAWLLANPGVEVSIDVESTTLTLPNGTQVKFPLEGF
                     ARFCLLNGVDELGFLLSQNAAITAYEHSHR"
     gene            complement(5307..6689)
                     /gene="leuC"
                     /locus_tag="VEW08_17670"
     CDS             complement(5307..6689)
                     /gene="leuC"
                     /locus_tag="VEW08_17670"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF004016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HYJ42624.1"
                     /translation="MFEKVWNDHVVVPETADTPAVLFIDLHLTHEVTTPQAYTELRTR
                     GIRLRRPDLTLATMDHSTPTRTEQVFGGVPIAVDSAARQVQQLEINAREFGVELFNLQ
                     DSRRGIVHIIGPELGLTQPGKTIVCGDSHTSTHGAFGALAFGIGTTEVGHVLATQCLL
                     QRRPKTFAINVVNDLPKGVTAKDLILAIIGKISVSGGTGYVLEFRGRAIERLSMDERM
                     TICNMSIEAGARAGMIAPDATTFEYLAATPRAPKGAEWDAAVARWSKLPTDAGATFDR
                     SIDIDASELVPMVTYGTHPGMVAPITGSVPMQGDAVFEKALKYMGLAAGQIISEVPVQ
                     NVFVGSCTNGRLSDLRNAARVLRGHKLAEGVKMLVVPGSQSVKRAAEAEGLHQVFLDA
                     GAEWRESGCSMCLGMNGDLVPTGHYSISTSNRNFEGRQGVGARTLLASPETAAASAIR
                     GRITDPREFN"
     gene            complement(6799..7755)
                     /locus_tag="VEW08_17675"
     CDS             complement(6799..7755)
                     /locus_tag="VEW08_17675"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HYJ42625.1"
                     /translation="MPIPATKYIWFNGKLVPWEKATVHVLSHALHYGSSVFEGVRAYE
                     TPRGPAIFRLRDHTHRLFESAKVYRINVPFTEDQINHACREVISQNGLARGAYLRPVA
                     FRGYGEAGVVPKIDPPIEVAIAGWEWGKYLGAAEDEGVDVCVSSWQRVAPNTIPAMAK
                     AGGNYLSSQLIGLEAKRLGFVEGIGLGPDGTVSEGAGENLFLVKDGVLYTPGMAHSLL
                     AGITRHTVITLAKDLGIEVREISIPREFLYMADELFFTGTATEVAAIRSVDAIQIGNG
                     KRGPITHKLQAAYFGLVQGKTQDPRGWLDYVDMNAPRAAVAG"
BASE COUNT         1394 a         2412 c         2604 g         1439 t
ORIGIN      
        1 gacatgcgcg ccatggtcgc gcgcctgccg aatgccgagc tgcacgaaat ctcttcggtc
       61 tacggacacg acgcctttct caaggagaac cggcggctcg cgccgatctt cgccaacgta
      121 ttgaatgaca atccaggaac ttccccgtga cgacttcgtt gactgcagac acacgcgcgg
      181 tacgcgccgg cctcgagaat tgcgacgtaa ccggtgccgt ggtgccgccg ctgcatcttt
      241 cctccaccta cgcgttccgc gcttttggcg agaagcgcaa atacgactac agccgttcgg
      301 gcaatccgac gcgcgacctg ctcgctgatg cgctcaacga tctcgaaggt ggcgcgggcg
      361 ccgtcattac ggggtccggc atgggcgcca tcacactggt cggccacatc ctccccgtcg
      421 gcgcgcgggt catcgctccg cacgattgct acggcggcac gttccgcctg tttacggcgt
      481 ggcagaagcg cggtgagctc gccgtggaat ttgtcgactt cggaaacaag gctgcggtcg
      541 atgccgcgtt gtcgaaaccc gcggcgctgg tgtggattga aaccccgagc aatccgctgt
      601 tgcgcatcac cgatgtcgca gacatcgcca aacgcggtca tgccatcggc gcgctcgtag
      661 tcgccgacaa caccttcctc tcgcccggct ggcagcagcc gatcgcgctc ggcgccgaca
      721 tcgtcgttca ctcgaccacg aagtacatca atggccacag cgacgtggtc ggcggcgcgg
      781 tgatcgccgc gaagaaggaa gtcgccgagc agctcgcgtg gtggggcaac tgcctcggcc
      841 tcacgggcgg cgctttcgac agctatctga cgcttcgggg cctgcgcacg ctgcacgtgc
      901 gcctgcgcga gcacggcagg aatgcccagg cggtcgccga gttcctggcg aagcagaagt
      961 ccgtcaagcg cgtgtacttc ccgggattgc cctcgcatcc cggccatgac atcgcaaagc
     1021 gtcagcaatc cggcttcggc gcgatcgtca cgatcgaagt gcagggcggc gtcgatggcg
     1081 cgaagcagtt ttccgaagcg ctggagctgt tctcgctggc ggagtcgttg ggcggtgtgg
     1141 agagcctgat cgcgcatccg gcgacaatga ctcatgccgc gatggcgccc gaagcccgcg
     1201 cggcggcagg cctggtcgat gggttgctgc ggctgtccat cggcatcgag tccacagaag
     1261 acctgctgcg cgacctgcag aacggcatcg ccgccctggg ctagtcagtc agctatctcg
     1321 ccgcaggtca cgttggtgac cgcggcggtg atggcgcccg cgcggtcaga ggccatgagc
     1381 accgcggcgt tggcgacttc ggccatcttt ggcattcgct tgagcatcgt cttctcggcg
     1441 atgcgggctt cgaaggcttc gcgcgtgacc cctgcgagcc gagcatgttc gccgatggcg
     1501 aaggcaacgc ccggcgcatc cggtgaaccc gaggaccgaa gcgcgactac ccgaattcca
     1561 tcaccgccca gatccacggc gagctgccgg cacaaccctt cgatggccgc acaggcgact
     1621 ccgaacccac cgctgccggt gtagggcttg cgcgccacct gcgcggtgag cgcgaggatg
     1681 acgccgctgt gccgcctggc catgtggcgg gcggcggcgg ttgccgtgag gaagtgcgtg
     1741 cgcacggcgc cttcgacagg ggcgacgaca tcctcgaccg acattccagc cagcgaatcg
     1801 ccctggggac cagcgagact gatgaggttg aatgaaacat cgacgcggcc atgagagccg
     1861 atgacggcgt cgagatgctt tgcaatggaa tcctcatcga gcgcgtccac ttttgcggcc
     1921 gccgccgaat ccccggcccg ggcgatttcc gtggcgagtg cctgtacctt ctcgagggtt
     1981 cgacctgcga gttcgacgtg agcaccttcg cgtgcaaatg cgcgcgccac cgcgctgccc
     2041 atggcgccag ccgcgccgta gatcaccgcc accttgcccg ctaacaagcc ggcgcgcgtg
     2101 gccggcgccg gcttcttgcc ggatgcggcg gtggaaatcg tcggcaccgt ggcggcggca
     2161 ccggccacga gaaaatcacg acgagaggcc gcttccatga gggtcacata cagcaggaga
     2221 cgggcggggt gtagcggccg gtaggtgcga cctcgccacc cttgccatac atgttgtgtg
     2281 gcctgaccca atccgtgagt gaatggtagg ggccgttttc attacgaccc agcggcatga
     2341 cttcgagaac gcggtagatg ccgaggaaat cttcgccgcc acgcgcgtag ttcgaatacg
     2401 tgtggaagat gtcaccggaa gcatcgcggt agaacacgct gttgcccgag atgtcctcga
     2461 tgcccggatc gcaatacgtg tagttgtaga agacgcgctg cgcggccagg tcttccggcc
     2521 tgaacgaaac cccgtagtcg tagttgaagt ccgagccgaa cgaggaataa aaaggcgtgc
     2581 gccattgcat tttcgtgcgc agggcctcga gctccggcat cgtggcgcgc gcaatggcca
     2641 ccaccgatac atcgtggttc tccaggtgtg gcagcagact ttcgagatga tcgactccca
     2701 gtgcgcatcc cacgcattgc agcggctggc ctggaccctg catgacgtgt tgcacgaaca
     2761 actggctgcg cccgcggaac agatcggcga atgagacccg gccattggga gaatcgaact
     2821 cgtagcgctt gtcgaccttc acccagggca gggcgcggcg tttttccgcc agcgcatcgc
     2881 ggcggcgcgt gaactccttt tcttcgagca gcagttgttt acgctcggca atccattttt
     2941 cccgattcac gatgcgattc atgacgtctc ctcagggttg gttctttttg cccttaccgc
     3001 tactggtcga acggccaaac agcaattcga catagcgata gaggtgatcg aggctgtcga
     3061 tcgccgccct ggagtcctgc cgggccttgg ccatgatgaa agcgccctgg atcaccgcct
     3121 gcatgtgcac ggcgagcgat tcactatcga cccctttcac cgcctgctcg cgcactacgt
     3181 cgtcgatgat ggcgcgcagg aagaacaagt gtttctcgat cgatgcggcg caggcgtcac
     3241 ggatggcagg gtaggtctcg tggacttcct ggatggtggt gcccgcgtag caggtccatt
     3301 cccagatatc gccgctcaga agctccttgc gaaaggccac atatccaagc agccggtcca
     3361 atggatccgc gagcctttga tacgaggcga gcgcaaagac ctgaaaagcg cggtcgttcc
     3421 attgcccggc ggcggcgatg gccagatctt ccttgctggc gaaatggtgg aagaagctgc
     3481 ccttggtgac gcccgcggcg gcacagacgt cttcgacgcg tgtggcggcg tagccattgg
     3541 cgcgcaccag gtcgatggtg gcggccagca gccgggttct ggagttctcg ggagtctcgg
     3601 ccatgtgtca ccgtaccagc gggtatggtg gaccataccg gcgagtacgt aggccgtcaa
     3661 gcgtgcggcg tcagctctgc aatgcagcga ccacggcgtc acccatgcgg cgagtggtca
     3721 tggccggccg gccgtgctcg gcgatgtccg cggtgcgcgc cccgttggcg atgcagcggt
     3781 cgacggcctg ttcgaccgtt gccgcttcgc gctccagctc gagcgaatgc cgcagcagca
     3841 acgcgacact gaggatcgtg ccgatcggat tcgcgatgcc tttgccggca atgtccgggg
     3901 ccgagccgtg gatgggctcg tacagcccgc gtttgccatc tcctatcgaa gcggacggca
     3961 atacaccgag agagctcgcc agcatggcgc cttcgtcggt caggatgtcg ccgaacatgt
     4021 tctcggtgag catgacgtcg aactcgcgcg gacgcttcag caggtgcatg gcggcggagt
     4081 cgaccagcat gtgctcgaga tccacctcgg gaaattccga atgaatgacg tgcgaggcga
     4141 cctcgcgcca caggcgagag gtctcgagca cgttggactt gtcgatggag acgaccttct
     4201 tgcgccgctg catggcgagg cggccggcga cacgcgtgat gcgctcgatt tcgctggcgg
     4261 agtaactgca gaggtccgtg gcgagagtcg gcgtgcgggt cttggcgccg aagtagatgc
     4321 ccccggtgag ttcgcgcacg aacaccaggt ccacgccgtc gagaatttcg ggcttgagca
     4381 ccgaggcgtc acgcaacgcc gggtgaagct tgatgggccg caggttggcg tacacgccga
     4441 gctcgcggcg caatcgaagc aggccctgct cggggcgaac ggcagccgat ggcccccact
     4501 tcgggccgcc gatggcgcct aacaacacgg cgttggccgc gaggcaggtg tcgagcgtgg
     4561 tctgcggcag cggatcgccg gtgaggtcga tggcgatgcc accgaacggc gcctcctcga
     4621 gaatgaattc gtgcccgaag cgtttgccga tctgttcgag cacacggacg ccttcagcga
     4681 cgacctcggg accgatgcca tcgcccgcga tgacggcgat ttttgctttc atcggtgact
     4741 gtgttcgtac gcggtgatgg cggcattttg cgagagcagg aagccgagct catcgacacc
     4801 attcaggagg cagaagcgtg cgaaaccttc gagcgggaat ttcacctggg ttccgttggg
     4861 cagggtgagc gtggtgcttt cgacatcgat ggacacttcg acacccgggt tggccagcaa
     4921 ccacgcgtgc gtctgctcat cgacgagcac gggcaggagg ccgttcttca ggcaattggt
     4981 gcggaatata tcggcgaact cggtgctgac gacggcctgg aatccaaaat cctgcagcgc
     5041 ccagggcgcg tgttcgcgag aggagccaca accgatgttg cgaccggcga cgagaattgc
     5101 ggcgcctttc gcttcagggc ggttcaacac gaactcgggc ttcggattgc cggcggcgtc
     5161 atagcgccag tcggcgaaca ggtgtttgcc gaagccagcc ttcgatgtgg aggtgagaaa
     5221 ccgcgccgga atgatctggt cggtgtcgac gttggtcgcg ggcaacgcga cggtgcggga
     5281 gcggatattt ttgacggcca ttttgatcag ttgaactcgc gcggatcggt gatacggcca
     5341 cggatggcgg aagcggcggc ggtttcgggg ctcgctagca gcgtgcgcgc gccgacgccc
     5401 tggcggcctt cgaaatttcg attgctggtg ctgatggaat agtggccggt gggtacgagg
     5461 tcaccattca tgccaaggca catcgagcag ccgctctcgc gccattcggc gccggcgtcg
     5521 agaaacacct ggtgcaggcc ttcggcctcg gccgcgcgtt tgacactctg ggagccgggc
     5581 accacgagca tcttcacgcc ctcggcgagc ttgtggccgc gcagtacgcg cgcggcattg
     5641 cgcagatccg aaagtcggcc gttggtgcag ctgccgacga acacgttctg cacaggcact
     5701 tcggaaatga tctgcccggc agcgagaccc atgtacttga gcgccttctc gaacacggcg
     5761 tcgccctgca tgggcacgga gccggtgatc ggcgcgacca tgcccggatg ggtgccatag
     5821 gtgaccatgg gcacgagctc gctggcatcg atgtcgatgg agcgatcgaa agtggcgccg
     5881 gcatcggtgg gtagtttgct ccagcgcgca accgcggcgt cccactcggc gcccttgggc
     5941 gcgcgtggcg tggcggccag gtattcgaaa gtcgtggcgt ccggagcaat catgccggcg
     6001 cgcgcgcctg cttcgatgga catgttgcag atggtcatgc gctcatccat cgacaggcgc
     6061 tcgatggcgc ggccacgaaa ttcgagcacg tagccggtgc cacccgagac gctgatcttg
     6121 ccgatgatcg cgaggatcag atctttcgcg gtgacgcctt tcggcagatc gttgacgacg
     6181 ttgatggcaa acgtcttcgg ccggcgttgc agcaggcact gggtcgccag cacgtgaccg
     6241 acttccgtgg tgccgatgcc gaaggcgagg gcaccgaatg cgccatgcgt cgaggtgtgg
     6301 gagtcgccac aaacaatcgt cttgccgggc tgggtcagcc cgagctcggg cccgatgatg
     6361 tgcacgatgc cgcgtcggct gtcctgaaga ttgaacagct cgacgccgaa ctctcgcgcg
     6421 ttgatctcga gctgctgtac ctgccgcgcc gcggagtcca ccgcgatggg cacgccgccg
     6481 aacacctgtt cggtacgcgt cggcgtcgaa tgatccatcg tcgcaagcgt gaggtccggg
     6541 cgtcgcaacc tgatgccgcg ggtacgcagt tccgtatagg cctgtggcgt cgtcacctcg
     6601 tgggtgaggt gcagatcgat gaacagaacg gcgggagtat cagcagtctc cggcacgacc
     6661 acgtggtcgt tccagacttt ctcgaacatc gtctttggtt ggctattcac ggggtcgtct
     6721 tggtgaagaa tggggtcagt aagaatgggg tcaggcaccg tttttcgaag acgaaaaacg
     6781 gagccagacc cctagctact aaccggcgac ggcggcccgg ggggcgttca tgtcgacgta
     6841 atcgagccag ccgcgcggat cctgggtctt gccctgcacc aggccgaaat aggccgcctg
     6901 gagcttgtgc gtgatcggtc cgcgcttgcc attgccgatc tggatggcgt ccaccgaccg
     6961 aatggccgcg acttccgtgg ccgtgccggt gaagaacagc tcgtcggcca tgtacaggaa
     7021 ttcgcgcgga atggagatct cgcgcacttc gatgccgaga tcctttgcca gcgtgatgac
     7081 ggtgtggcgc gtgatgccgg cgagcagtga atgcgccatg ccgggcgtat agagcacgcc
     7141 gtccttcacg aggaagagat tctcgccggc gccttcgctg accgtgccgt ccgggccaag
     7201 cccgatgcct tcgacaaagc cgagacgctt cgcctcgagg ccgatgagct ggctggagag
     7261 gtaattgccg ccggccttgg ccatggcggg aatcgtgtta ggcgcaacgc gctgccacga
     7321 cgacacgcac acatcgacgc cttcgtcttc ggcggcgccg agatatttgc cccattccca
     7381 gccggcaatg gcgacttcga tcggtgggtc gatcttgggc acgacaccgg cttcgccata
     7441 cccgcgaaat gcgaccgggc ggaggtacgc gccgcgcgcc aggccgttct gcgaaatgac
     7501 ctcccggcag gcgtgattga tctggtcttc cgtgaaggga acgttgatgc gatacacctt
     7561 cgccgactcg aacaggcggt gcgtgtgatc gcgcagccgg aagatggccg gaccgcgcgg
     7621 cgtttcgtag gcacgaacgc cttcgaacac cgacgagccg taatgcagtg cgtgcgagag
     7681 cacgtgcacg gtcgcctttt cccaagggac cagcttgccg ttgaaccaga tgtatttcgt
     7741 tgcgggtatg ggcatttgcc tgaactccga atttgttaga tcgccgcctg cgcgacacgt
     7801 gagcgcgaga ttgcaccctg ggcgagctcg atgcggttga taacttcga
//
LOCUS       DASQJS010000448        14504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4375702, whole genome shotgun sequence.
ACCESSION   DASQJS010000448 DASQJS010000000
VERSION     DASQJS010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14504
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4375702"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      221..428
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            531..2375
                     /locus_tag="VEW08_17680"
     CDS             531..2375
                     /locus_tag="VEW08_17680"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ42626.1"
                     /translation="MNRNSSTQVALAVAAALVSPTIFAQDDLDETVVTATRTPVALDA
                     VGAPVIVITRDDIERSLASDVSELLQAHAGLEIARNGGPGQTTSLFTRGTESNHTVVL
                     IDGVRINPGTIGGAALQNIAPESIERIEIVKGPRSSLYGTDAIGGVVQLFTRGAAKDG
                     ASAGATFGSDNTQQVFGDFALTTGEKLRLGFGGSYAESEGMPVFVDDNTDRGYRNVTG
                     RASLEYSATDELTLRARAWDTSGRTEYSSQTFSDPPYAAVSQDFENSVYSAEVEYRRA
                     EGFGVRATVSHALDDIEQLQPGFGPAEFDYARTARTNVDVQMDLAQVGTHAFTLGLQH
                     SDENTRAQSFGTVFDEDTSVMQAFVQDQFEVGRLTSRLALGGVDHETFGNELTWNAEF
                     SAAFDTGTRMTLSGGTAFRAPDSTDRFGFGGNPDLGPEVSEQVEFSVRQKLGARHQLA
                     LSAFDNHIDDLIEYVVTDFVTFEGQNQNVERARIKGVELGYNFTGEAWRARAELTLQD
                     PRDETTGERLLRRSREALMVAVNRDVGALDVGVDIVAVGDRKDFGFPAPVTLDSYTLV
                     NATLRYRVNDALTLQGRFENIFDEDYTYAEGYRTEGASYTVGVRYSFE"
     gene            2399..2959
                     /locus_tag="VEW08_17685"
     CDS             2399..2959
                     /locus_tag="VEW08_17685"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HYJ42627.1"
                     /translation="MGNRLSRIYTRTGDDGTTGLGDGARVRKDHIRVEAYGTVDEANS
                     AIGVILSVPGLPAEVARCLTEIQHDLFDLGGELCIPGTKAIRAEQIDKLEQVLDKFNE
                     PLPALKDFILPGGGPAAAHCHLARTIVRRAERCVWTLAGSEQVNVEVPKYLNRLSDLL
                     FVLARVLARHEHGSEVLWRHDRGAGR"
     gene            2984..3544
                     /locus_tag="VEW08_17690"
     CDS             2984..3544
                     /locus_tag="VEW08_17690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HYJ42628.1"
                     /translation="MSEADSLTDLIRRAQDGDEAALRIVFDATYAELRGLARARLRKG
                     SRNTLLDTTSLVHEAFLRFADAGRLHIGDRQHFLRYASHVMRSVIVDFVREKLAQRRG
                     GNAVHVTLDSQIGDAPNAGEREILQVHEALEELAQLDPRMVQVIEMRYFAGMTEAEIA
                     AALGVNDRTVRRDWQKAKLLLADALK"
     gene            3620..6319
                     /locus_tag="VEW08_17695"
     CDS             3620..6319
                     /locus_tag="VEW08_17695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYJ42629.1"
                     /translation="MQKLQIDASTWSTLNRLLDEALDKPAAQLPQWLDELAPEFEGLK
                     PRLRDLLSRTGLIETGEFLHTLPKFELEPDDLAAASGEHAGQEIGPYRLVRELGSGGM
                     GVVWMAERTDGLIDRPVALKLPHGAWKRAGLAERMARERSILATLAHPNIAHLYDAGI
                     TADGQPYLAIEYVEGRRIDYFCDEQSMDVKARLRLFAQVADAVAYAHAKLVVHRDLKP
                     ANILVSAGRVKLLDFGIAKLLEDGEARETRVTELSGRALTPDYASPEQLRGEPLTIAS
                     DVYSLGVVLYELLCGQRPYKLAHDSRGALEDAILQAEPPAPSGVVPSARRRELRGDLD
                     TIVLKALKKKPGERYATVHALADDIGRFLDGHPVLARPDSWTYRTSRLLRRHRLVFSA
                     STAVAVVLVGATLYSMRQAQLARTEELRAGQVEQFIASILTDTQSAESPTAEGLLKQA
                     RARIGDEFADHPLTQIRLLLIVSRSLIGFSEYDAADEALDAALSISRDHPDAADSYVR
                     QARVQRLSLHRFRGRLVQLRQELPALRSEMDRDGNWSPADRVKLLIEAAYSANLDALD
                     ERAAVDIQTAADLALRELGPRHELTLEAASARSAILRGANRYDEAIAAAEQALALMIS
                     AYAPNLYHRRILDARMALGTSYLSKGRIHDAVRELGTVIDHAGDRDFLELYAQVHITR
                     AWVSGGEPQAALRALARAESLALDSNNADTVILSQAMALRAYVLASMGEMSPALQVID
                     EALKRYGREHARDHPPTLAFRALRAMILAHLARIGEARAELEEISGLNEISGKKSSIL
                     HWTLPHLSYAKGVVERLAGNCDLALTLQQEALQSIRADPGTWLKRARILGEIGLCAPA
                     GQKARAVTSLDQALSLLREHQTSGTSSDRFTLELQAKLASLTH"
     gene            complement(6359..6790)
                     /locus_tag="VEW08_17700"
     CDS             complement(6359..6790)
                     /locus_tag="VEW08_17700"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HYJ42630.1"
                     /translation="MTDVRTGLMWERCMMGYTFSDNGTPTDLTDDSCTATATTTFIWQ
                     QALQGAADRGFAGFTDWRLPNIKELISIVEHMCAGPAINETIFPGTPPTAEVFSSSSG
                     NDGLSKTTVMLLHMADGQDDSTSNAVIQTAAKSVRLVRVAN"
     gene            complement(6945..9731)
                     /locus_tag="VEW08_17705"
     CDS             complement(6945..9731)
                     /locus_tag="VEW08_17705"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HYJ42631.1"
                     /translation="MTCRRCNQRRSAVLTLTFFALFSPLACDAQGAANAWAIFESGPL
                     LDDPGPYLVAESIRTAGVPGLLGSVPFPMSVGFPDALGIRAPLTAFATEAGLVRVLAA
                     ADDPNKELEAGSVQLIWRRTFTKGLPSDIATFTISLTSLHLILAGQPVDDVIPSSGYT
                     MTVRLAPGDLHQIGVNEEQELGWQEQFRHEVKLQANDLTPFLSVEKTSIGGAVVYDLA
                     SNGQSGRVMKLAPTTDIPGGYYQTSRFTGVVNLGRITTGTLYTVEYDIRIEVQKVFRE
                     GLSQGIVQDPLDVDGGFRFETTSAPADDSLEATCDAPLDAARFLDNGDGTVNDAHTGL
                     MWQRCPLGYVLNAQGTTADLRDDTCDVAGSSPGNWQAALQAAASDALAGHGDWRVPNL
                     KELESIVQLQCIAPTIDTITFPFAAPTITWSSTPDPVSGAAARIVDFALGRVSSIDKT
                     ASAETRLVRDSGEAPRRAAPAISVGRAAAVNESDAGTVALSFPIALDAVAATDVSVEY
                     ETRDLSALAGEDYVSTSGVATIPAGSRQVIVQVPVINDSLGEFNQALLMRLSAPSASA
                     RLRIDRAVGQIDDDEPVLTIAGANITEGDAGQVSLVFPVTLSGVTTDDVTVQFATADG
                     TATAGADYVASSGTLRIPAGTTSGSVVVPVSADALIEGDETLTVTLSNTSANTRLAID
                     SALGSIVDDDAVTLRALNDTGITLCANESNAAVACSQTSAFPGQDAEFGRDATANNNA
                     DGAAGFSFTKLDATGTPLIDQAVAYDVSPWDCVQDEVTGLQWEVRTDNGGLRDKDWTY
                     TWFNSTGINDGGSAGTANGGVCVDGSNCDTEKYVSAVNAVGLCGRTDWRLATREELQS
                     IADISRFSTAPRYDINFFANPAPTSVATSHWTSTPDASAPGIAWSVDKLKGRGSAIFA
                     KSRAFSLILVRGGN"
     gene            complement(9731..10924)
                     /locus_tag="VEW08_17710"
     CDS             complement(9731..10924)
                     /locus_tag="VEW08_17710"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HYJ42632.1"
                     /translation="MEFITLRMCTYSRRFVIAVLLIMVAVTLAACSGRGDDGGEEPQP
                     QPTPAIPPQLSIGIVATAEGNSGVTNFVLTVTSSAAASSNLTATFTTADGTATAGSDY
                     TAGSGTLTIAAGSTAANITIAINGDAVFEADETFTVTLTGASGATLGTASAIVTIIND
                     DAQPAPLGAGLNDTGVTTCSNGVNIGLGCNSAVVGTDVFPGQDAEHGRDVTTNTAADG
                     HAGFSFTKLDANGVPLPDQSVTFSAAPWHCVLDNVTGLLWEVKSDDGGLRDKDWRYSW
                     FDSSGVADGGDTGRENLGVCVNSSDCDTEKYREAVNAAALCGRTDWRLPVRSELLSII
                     HYGSGSAPFIDVDFFPQSVSASHWSGSVRGSEAWAVSLLTADALLVAKDALRAARLVS
                     DGGGS"
     gene            complement(11048..11416)
                     /locus_tag="VEW08_17715"
     CDS             complement(11048..11416)
                     /locus_tag="VEW08_17715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42633.1"
                     /translation="MVVRLIAGTGAGPTNHAAYEAALQAAGHDQRLCVAQSHMAQSQT
                     GSWAHAALGWLRDERTGCATFIELQHDNLEHLQRELHAAASSRRRQSAMALGPLQARF
                     ASVPCRGQPVCALVLAHRVE"
     gene            complement(11447..11866)
                     /locus_tag="VEW08_17720"
     CDS             complement(11447..11866)
                     /locus_tag="VEW08_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42634.1"
                     /translation="MKRRNFFTALAVPLGIALTVKTGDVFAYDNPRRWPPVRRRIRRP
                     VVVRTSSGRPCWVVPVGLVPGWELSHDDRVVVVRETHFIEKAGVKSEVAIVQDTSDRI
                     EQVEITREDTAENSRVLQGSVLDDGDTTTPAIASASR"
     gene            complement(12098..12415)
                     /locus_tag="VEW08_17725"
     CDS             complement(12098..12415)
                     /locus_tag="VEW08_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42635.1"
                     /translation="MIRKLLLTSLGCLYAASALGVGACQGQACRYTSFGKDADGCLEI
                     RNLSREDIEVTVYTAASGTYTVRVAGGDTEKIYKTGRMCVPAADYLRSGAEYAGGIFS
                     PSR"
     gene            complement(12439..12972)
                     /gene="ampD"
                     /locus_tag="VEW08_17730"
     CDS             complement(12439..12972)
                     /gene="ampD"
                     /locus_tag="VEW08_17730"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HYJ42636.1"
                     /translation="MRVDVSTGLMVGVKQVLSPFFDERPAGVVPDLLVLHGISLPPGE
                     FGGPWIARLFTGNLPAAAHPEFRERAALRVSTHLLVRRDGRVEQFVSFNDRAWHAGKS
                     VWQGREACNDYSIGIECEGTDEAPYEAAQYETLRTLLPLLLEAYAIPRDRIVGHSDIA
                     PGRKTDPGPSFEWSRVR"
     gene            13029..14402
                     /locus_tag="VEW08_17735"
     CDS             13029..14402
                     /locus_tag="VEW08_17735"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:NF005443.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HYJ42637.1"
                     /translation="MARTESLMSSGNSDWVTRDLAHVWHPCTQMKDHESVPMIPLRSG
                     KGVWLEDFTGKRYLDGISSWWVNLFGHANPRINAAVREQLEQLEHAIFAGFTHPPAVQ
                     LAEELVRVTPAGLNRCFFADNGSAAVEVAVKMSFHYWRNTGHQRKTRFITLANSYHGE
                     TLGALAVGNVDLYKSIYRPLLMDVITVPSPDAFMREPGVTAAEHARKMFAHMEAAIAQ
                     NHGEVAAVIVEPLVQCAGGMRMYDPVYLKLLREACDRHQVHLIADEIAVGFGRTGTMF
                     ACEQAGIRPDYLCLSKGLTGGYLPLSAVLTTDGIYAAFYDEYTRLNAFLHSHSYTGNP
                     LACAAANATLAIFREEPVIERNRATAAHMLTAVGHLHDHPHVAEIRQCGMILAIELMK
                     DPATRTPWPWEQRRGLRIYRHALEHGALIRPLGTTIYFMPPYVITREEIDLLSRVATE
                     GIDLACA"
BASE COUNT         2682 a         4684 c         4479 g         2659 t
ORIGIN      
        1 gagcaggtca cgaatggcct cttcggagcg cacgtcggcc tcggtgaccg gggtcttgtc
       61 cgccttcacc tcgatcttca cgttgcgttg gtagaaaccg cggatcactt ccgcggcggc
      121 gcgcgccgcg tcgagggcgg cattgagttc attcgacatt tcggggttcg gcatggcggg
      181 cgaagcctgc ggtagctgcg ccggaactgt caattcgcaa cgaatctcgc tagagttcgg
      241 cccgccgaaa ggttacggga agctggtgag ccgccattag atggcaattc cagcactgcc
      301 cccgcaacgg taatcgaacc gcaactcgtc aattgccact gagccgcaag gcttgggaag
      361 gcgacgcgtc ggaacagcga tgttccagtt cgagagtccg gagaccggcc tgacggcatc
      421 attcccagtt gcggcgggca acgggacggg cttcatgccc ttcctgtccg ctctcatcct
      481 gggtgttcaa gcattcggcc atgcgggcgt gcatggaaag ggcatttttc atgaatcgca
      541 actcatctac gcaagtggcg ctcgccgtcg ctgcggctct cgtttcaccc acgatcttcg
      601 cgcaggacga cctcgacgag accgtggtca ccgcgacgcg cacccccgtg gcgctcgacg
      661 cggtcggtgc accggtcatc gtcatcacgc gcgacgacat cgagcgttca ctcgccagcg
      721 acgtcagcga gctcctgcaa gcgcacgccg gcctggaaat cgcgcgcaac ggcggccccg
      781 ggcaaaccac gtcgctgttc acgcgcggca ccgagagcaa ccacaccgtg gtgctcatcg
      841 acggcgtgcg gatcaacccg ggcaccatcg gcggcgccgc gttacagaac atcgcgcccg
      901 aatcgatcga gcgcatcgaa atcgtcaagg gcccgcgctc ttctttatat gggaccgatg
      961 ccatcggcgg cgtcgtgcaa ttgttcacgc gcggcgcggc caaggatggt gcgagtgccg
     1021 gcgccacttt cggcagcgac aacacccagc aggtgtttgg cgattttgct ttgacgacgg
     1081 gcgaaaaact gcggctcggt tttggcggca gctacgccga aagcgagggt atgccggtgt
     1141 tcgtcgacga caacaccgat cgcggctatc gcaatgtcac cggccgggcg tccctcgaat
     1201 actccgcgac ggatgagctg accctgcgcg cgcgcgcctg ggatacctcc ggccgaacgg
     1261 aatattcttc acagaccttc tcggatccgc cgtacgcggc cgtctcgcag gacttcgaaa
     1321 acagcgttta ctcggcggaa gtcgaatacc ggcgcgccga aggtttcggc gtgcgcgcca
     1381 cggtgagcca cgcgctcgac gacatcgagc agctgcaacc ggggttcggt ccggcggaat
     1441 tcgactacgc gcgcaccgcc cgcaccaacg tcgacgtgca aatggacctg gcgcaggtcg
     1501 gcacgcatgc gttcacctta ggcctgcagc acagcgacga aaacacccgg gcgcaatcct
     1561 ttggcacggt gttcgacgaa gacacctcgg tgatgcaggc attcgtccag gatcagttcg
     1621 aggtcggccg cctcacctcg cggctggcgc tcgggggtgt ggatcatgag accttcggca
     1681 acgagctcac ctggaacgcg gaattcagcg ccgcattcga cactggcaca cgcatgacgc
     1741 tctcgggagg caccgcgttc cgcgcacccg acagcaccga tcgattcggc ttcggcggca
     1801 acccggatct cggccccgag gtttcggagc aggtggaatt ttcggtgcgc cagaagttag
     1861 gcgcccgcca ccagctcgcg ctgtccgcgt tcgacaacca catcgacgat ctcatcgaat
     1921 acgtggtcac cgacttcgtc acgttcgaag ggcagaacca gaacgtcgag cgcgcgcgca
     1981 tcaagggcgt ggagctcggg tacaacttca cgggtgaagc gtggcgcgcc cgcgccgagc
     2041 tcaccttgca ggatcctcgc gacgagacga ccggcgaacg cctgctgcgc cgctcacgcg
     2101 aagcgctgat ggtggcggtg aatcgcgacg tgggtgcgct cgatgtgggc gtggacatcg
     2161 tcgcagtcgg cgaccgcaag gatttcgggt tcccggcacc cgttacgctc gattcctaca
     2221 cgctggtcaa tgccacgctg cggtatagag tgaacgacgc gctcacgttg cagggccggt
     2281 tcgagaacat cttcgatgag gactacacat acgccgaggg ctaccgcact gaaggtgcct
     2341 cgtacaccgt tggagtacgc tacagcttcg aataactctc gcagcgcagg gtggcggtat
     2401 gggcaatcgc ttgagcagga tctatacacg cacaggcgac gatggcacca cgggcctcgg
     2461 cgacggagcg cgggttcgca aggatcacat ccgtgtcgag gcctatggca ccgtggacga
     2521 ggccaatagc gccatcgggg tgatcctcag cgttcccgga ttgcccgcgg aagttgcccg
     2581 atgcctcacg gaaatccagc atgacctgtt cgatctcggt ggagagttgt gcattcccgg
     2641 cacgaaggcc atcagggcgg aacagatcga caagctcgaa caggtgctcg acaagttcaa
     2701 cgagccgctg cccgcgctga aggatttcat actgccgggc ggcggccccg ccgccgcgca
     2761 ttgccacctg gcgcgaacca tcgtgcggcg cgccgagcgc tgcgtgtgga cgctggcggg
     2821 atcggagcag gtcaacgtcg aagtgccgaa gtacctgaac cggttgtcag atctgctgtt
     2881 cgtgctggcg cgcgtgctcg cgcggcatga acacggttca gaagtcctgt ggcgccacga
     2941 ccgcggcgcc ggccggtaaa gctgcgtcag gcaacggact gccatgagcg aagccgattc
     3001 actcactgac ctcatccggc gcgcacagga cggcgacgaa gcggcgttgc gcatcgtgtt
     3061 cgatgcaaca tacgcggaac tgcgcggact ggcgcgagcg cggctgcgca agggcagccg
     3121 caataccttg ctcgatacga cctcgctggt gcacgaggcc ttcctgcgtt tcgccgacgc
     3181 gggccgcctg catatcggcg atcgacagca tttcctgcgt tatgcaagcc atgtgatgcg
     3241 ctcggtcatc gtcgacttcg tgcgcgaaaa gctggcccag cggcgtggtg gcaatgccgt
     3301 gcatgtcacg ctggactcgc agatcggcga cgcgccgaat gccggcgaaa gggagatctt
     3361 gcaggtacac gaagcgctcg aagagctggc gcaactcgac ccgcgtatgg tgcaggtgat
     3421 cgaaatgcgc tatttcgcag gcatgaccga ggccgagatt gccgccgcgc tgggagtaaa
     3481 cgaccgcacg gtgcgccgcg attggcaaaa ggcgaagctg ctgcttgccg atgcgctgaa
     3541 gtagtcgacg ccggactgtc cggatcttgc gcgaaattcc gttattgccc ggctggagca
     3601 attttcacgg ggcgctgtca tgcagaagtt gcagatcgat gcgagtacct ggtcgacatt
     3661 gaatcgtctg ctcgatgagg cgctcgacaa gcctgccgcc caactaccgc aatggctcga
     3721 cgagctggcg ccggagttcg agggcctgaa gccgcggctg cgcgaccttc tctcgcgtac
     3781 cggcctgatc gaaaccggtg agtttctgca cacgctgccg aagttcgagc tcgaaccgga
     3841 cgaccttgca gccgccagcg gcgaacacgc aggccaggaa atcgggccat atcgactggt
     3901 acgcgagctc ggcagcggtg gtatgggtgt ggtgtggatg gcggaacgta ccgacggtct
     3961 catcgatcgg ccggtagcac tgaagctgcc ccacggcgcc tggaagcgcg ccggtctcgc
     4021 ggagcgcatg gcgcgcgagc gctcgatcct cgccactctc gctcatccga acatcgcgca
     4081 tctgtatgac gccggcatca ccgccgatgg ccagccctat ctcgccatcg agtacgtcga
     4141 agggcggcgc atcgattact tctgcgatga gcagtcgatg gacgtgaagg cccgactgcg
     4201 gctgtttgcg caggtcgcgg acgccgtggc gtacgcgcac gcaaaactcg tcgtgcatcg
     4261 cgatctcaag ccggcaaaca tcctcgtcag tgccgggcgg gtgaagttgc tcgacttcgg
     4321 cattgccaag ctgctcgagg acggtgaagc gcgcgagacg cgggtcaccg agctgtcggg
     4381 gcgcgcgttg acacccgact acgcttcgcc ggagcaattg cgtggtgagc cgctcaccat
     4441 tgcctcggat gtgtactcgc tgggcgtcgt actgtacgaa ctgctgtgtg gccagcgtcc
     4501 atacaaactt gcgcatgatt cccgcggcgc gctcgaggac gccattctgc aagccgagcc
     4561 gccggcaccc agcggcgtcg tgccttccgc gcgccgccgg gaattacgcg gtgatctcga
     4621 caccatcgtc ctcaaggcgc tgaagaaaaa accgggcgag cgttacgcaa ccgtgcatgc
     4681 gctggcggac gacattggcc ggtttctcga tggccacccg gtgctggcgc gccccgactc
     4741 gtggacctat cgaacgtccc gtttattgcg tcgacacagg ctcgtttttt cggcgagcac
     4801 cgcggtggca gtcgtgctcg tcggcgccac gctgtactcc atgcgccagg cgcaactcgc
     4861 ccgtaccgaa gagttacgcg caggacaggt cgagcagttc attgcgtcga ttctcaccga
     4921 tacccagtct gccgaatcac cgaccgcgga aggactgctg aagcaagccc gcgcccggat
     4981 aggcgacgag tttgccgatc acccgctgac acagattcgt ttgctcctca tcgtcagccg
     5041 aagcctcatc gggttctccg agtacgacgc cgccgacgaa gccctggacg cagctctgtc
     5101 catctcgcga gaccatcccg acgccgcgga ctcctatgtc cggcaggcgc gcgtgcagcg
     5161 actgtcgttg caccgcttcc gcggcagact cgtccaactt cgccaggaac ttcccgcgtt
     5221 gcggtcggaa atggatcgcg atggcaactg gtcacctgct gaccgcgtca aacttctcat
     5281 agaagcagca tattcagcca acctggatgc cctggatgaa cgggccgccg tcgacatcca
     5341 gactgccgcc gacctcgcac tccgggagct gggccctcgt cacgagctca cgctcgaggc
     5401 cgcaagcgcc cgcagcgcga tcctgcgagg agccaaccga tacgacgagg caatagcagc
     5461 ggctgaacag gcccttgcac tcatgatcag cgcatacgca cccaacctgt atcaccgccg
     5521 gatactcgac gcgcgaatgg cgctcggcac cagctacctg agcaagggta ggattcatga
     5581 cgccgtacgg gagctgggca ccgtgattga tcacgccggc gatcgcgatt ttctcgagct
     5641 ctatgcgcag gtccatatca ctcgcgcctg ggtttcaggc ggcgaaccac aagctgcgct
     5701 gcgggccctg gcccgcgccg aatcactcgc gctcgactcg aacaatgccg atacagtcat
     5761 cctgagtcag gccatggcgc tgcgcgccta tgtgctcgcg tcgatgggag aaatgtcgcc
     5821 ggcattgcaa gtcatcgacg aggcgttgaa gcgttacggg cgcgaacatg cccgtgacca
     5881 ccctccgacg cttgccttcc gcgcgctcag agcaatgatt cttgcgcacc tggcgcgcat
     5941 tggagaggcc cgcgccgaac tcgaggagat ctccgggctc aatgagatct ctggaaagaa
     6001 gtccagcata ttgcactgga ccttgccgca tctgtcttac gccaaagggg ttgttgaacg
     6061 actcgctgga aactgcgatc tcgcgctcac cttgcagcag gaagcgctgc agtcgatcag
     6121 ggcagacccc ggcacgtggc tgaagcgcgc cagaattctc ggcgaaatcg gcctgtgcgc
     6181 accggccgga cagaaagccc gcgcagtcac atcactagac caggcgcttt cgctgcttcg
     6241 tgaacatcaa acgtccggga catccagcga tcgctttacg ctcgaactgc aggcaaagct
     6301 ggcgagcctg actcactgaa agtcgcgaat ccggacacgc ctcttgtccg acaggctgct
     6361 agttcgccac gcgcaccagg cgcacggact ttgccgcagt ctgaatgacc gcattcgacg
     6421 tggagtcatc ctggccgtcg gccatatgca aaagcatcac ggtagtcttg ctcaaaccgt
     6481 cgtttccgct cgaggaactg aacacttccg cggtcggtgg cgtgcccgga aatatcgttt
     6541 cgttgatcgc cggacccgcg cacatgtgtt cgacgatgga tatgagctcc ttgatgtttg
     6601 gcagccgcca gtctgtgaat ccggcgaacc cgcgatccgc ggcgccctgc aacgcctgct
     6661 gccagatgaa cgtcgttgtc gccgtcgcag tgcaagagtc atccgtaagg tcggtcggag
     6721 ttccgttgtc actgaaggtg taccccatca tgcagcgttc ccacatgagg ccggtacgga
     6781 cgtcagtcac cgttccattg ccgttgttgg tgaatcggct cgtgggggtg gactgattga
     6841 ttctgtcatt acatgcctgt tgcgcattga cggcggtgca taacagcagc gggacgagaa
     6901 taaggatgcg tgctcgcatg atggatatct ccgattgatt ttgctcagtt cccgccgcgg
     6961 accaggatca gtgagaacgc cctgcttttt gcgaagatgg cggaacccct gcccttcagc
     7021 ttgtcgaccg accaggcgat gccgggagcc gatgcatcgg gcgtcgaagt ccaatgcgac
     7081 gtcgccaccg atgtaggtgc cgggttcgca aaaaaattga tgtcgtagcg cggcgccgta
     7141 ctgaatctgc tgatatcggc gatcgactgc agctcctcgc gcgtcgccaa ccgccagtcg
     7201 gtccggccac acaaaccgac cgcattgacg gcgctgacgt acttctcggt atcgcagttg
     7261 ctgccatcca cacacacgcc gccgttggca gttccagcgc taccgccgtc attgatgccg
     7321 gtggagttga accaggtgta cgtccagtcc ttgtcacgca agcccccatt gtcggtcctg
     7381 acttcccact gaagaccggt gacttcgtcc tgcacgcagt cccatgggct gacgtcgtat
     7441 gcgacggcct ggtcgatcaa cggagtaccg gtcgcatcga gcttcgtgaa cgaaaagccg
     7501 gcggcgccat cggcgttgtt gttagccgtc gcatcccggc cgaactcggc gtcctgtcct
     7561 ggaaaagccg aagtctggga gcaggcgacg gcggcattgc tctcgttcgc gcagagtgtg
     7621 atgcccgtgt cgttcagcgc gcgcagcgtg accgcatcat cgtcgacgat gctgcccagc
     7681 gcgctgtcga tggctaaacg agtgttcgcg gacgtgttgc tcaaggtcac agtcagggtt
     7741 tcatcacctt cgatcagtgc gtcggcgctc acgggaacca cgacgctacc cgacgtagtt
     7801 cccgccggaa tgcgcagcgt gcccgaactc gccacgtagt ccgcgcccgc ggtggccgta
     7861 ccatcggcgg tcgcgaactg cacggtgaca tcgtccgtcg tcacaccact cagcgtgacc
     7921 ggaaacacca gcgacacctg gccggcgtcg ccttcagtga tatttgcgcc agcgatcgtc
     7981 aggaccggtt cgtcgtcgtc gatctggccg acggcccgat cgattcgcag gcgtgcgctt
     8041 gcggatggcg cgctgagtcg catgagcagc gcctgattga actcgcccaa gctgtcgttg
     8101 attaccggga cctgcacgat cacctgtctg ctaccggctg gaattgtcgc gacgcctgac
     8161 gttgacacgt agtcttcgcc ggcgagtgcc gaaagatccc gggtctcgta ctcgacgctg
     8221 acatccgtag cggcaaccgc gtcgagtgcg atgggaaagg acagtgccac tgtgccggcg
     8281 tcactttcat tgacggccgc ggcacggccg acgctgatcg ccggcgcggc ccggcgcggg
     8341 gcttctccac tgtcgcggac caaccgggtt tctgcgcttg cggtcttgtc gatcgacgag
     8401 acccggccca aggcgaagtc gacgatcctg gctgcagcac cactcaccgg atcgggcgtg
     8461 gaggaccagg tgatggtggg ggccgcgaat ggaaacgtga tggtatcgat cgtcggcgcg
     8521 atgcattgca attgaacgat ggactccagc tccttcaggt tcggcacccg ccagtcgcca
     8581 tggcctgcaa gcgcatcgct agcggccgcc tgcagcgccg cctgccagtt accaggcgaa
     8641 ctgcctgcca cgtcgcaggt gtcgtcccga agatcagcag tggtgccctg tgcgttgagc
     8701 acgtagccca aggggcagcg ctgccacatc aaccccgtgt gtgcgtcatt gacggtgccg
     8761 tcgccgttgt cgaggaagcg agccgcgtcc aggggtgcat cgcaagtcgc ctcgagcgaa
     8821 tcgtccgccg gcgcgctcgt ggtctcgaag cggaatccgc cgtctacgtc gagtggatcc
     8881 tggacgattc cctgcgaaag tccttcgcgg aaaaccttct gcacctcgat gcggatgtcg
     8941 tattcaaccg tgtacagcgt gcccgtcgtg atcctgccca gattgacgac gcccgtgaag
     9001 cggctcgtct ggtagtagcc cccggggatg tcggtcgttg gcgccagctt catcacgcgc
     9061 cctgattggc cgttgctggc gagatcgtag accacagcac cgccaatgct cgtcttctcg
     9121 acactgagga acggcgtcag atcattcgcc tgcagtttca cctcgtgcct gaactgctcc
     9181 tgccagccga gctcctgctc ctcgttgacg ccgatctggt gcaagtcgcc cggggccagg
     9241 cgcacagtca tggtgtagcc gctcgatggg atcacatcgt cgaccggctg cccggccagg
     9301 atcagatgaa gagaggtgag actgatggtg aacgtggcga tatcgctcgg caggcctttc
     9361 gtgaacgtcc tccgccagat cagctggacg ctgccggcct ccagctcctt gttagggtca
     9421 tccgcagcgg caagtacgcg cacgagaccg gcctcggtgg caaacgcggt gagaggcgcc
     9481 ctgataccca aggcgtccgg aaaccccaca ctcatcggga atgggacgct gccgagcagg
     9541 ccgggcacgc cggcagtgcg aatgctctcg gcgaccaaat atggaccggg atcatcgagc
     9601 agcggtcccg attcaaaaat tgcccatgca ttcgccgcgc cctgcgcatc gcaggcaagc
     9661 gggctgaaga gcgcgaagaa ggtgagcgtc agaaccgcac ttcggcgctg gttgcaccgg
     9721 cgacaagtca tcacgatcca cctccatcac tcaccaggcg cgctgcccgg agagcgtctt
     9781 tggcaacgag aagggcatct gcggtcaaca gactgacggc ccacgcctcg ctgccccgga
     9841 cggagcccga ccaatgactc gcgctcacgg actgcggaaa aaaatcgacg tcgatgaaag
     9901 gtgcgctgcc cgatccatag tggatgatcg acagcagttc ggaccgtacc ggcagccgcc
     9961 agtcagtgcg tccacatagc gccgctgcat tgaccgcttc gcgatacttc tcggtgtcgc
    10021 aatcggacga attcacgcag acaccgagat tctccctgcc ggtatcaccg ccatcggcga
    10081 cgccgctgga atcgaaccag ctgtagcgcc agtccttgtc gcgaagcccg ccgtcatcgg
    10141 acttcacttc ccacaggagc cccgtgacgt tgtcgagcac gcaatgccac ggcgcggcgc
    10201 tgaatgtcac cgactggtcg ggcaatggca ccccgttcgc gtccagcttg gtaaacgaaa
    10261 atcccgcgtg accatccgcg gcggtattcg tcgtcacgtc gcgcccgtgt tccgcatcct
    10321 ggcccgggaa cacatcggtg ccgaccacgg cggaattgca gcccaagcca atgttgacgc
    10381 cgttcgagca ggtagtgacg ccggtatcgt tcaggcccgc accaagtggc gctggctggg
    10441 catcgtcatt gatgatcgtg acaatcgcgc tggcggttcc cagcgtggcg ccggaagcgc
    10501 ctgtcaacgt gacggtgaaa gtttcatcgg cttcgaaaac cgcgtcaccg ttgatggcta
    10561 tggtgatatt tgctgcggtc gatccggccg cgattgtcag cgtgccgctg ccggcggtgt
    10621 agtcgctgcc tgccgtggcc gtgccgtcgg ccgtcgtgaa ggtcgccgtc aagttgctgc
    10681 tcgcagcggc actcgacgtg accgtgagca cgaaattggt gacaccgctg tttccttccg
    10741 cagttgcaac gatgccgatg ctcagctgcg gaggaatggc aggcgtgggt tggggctgcg
    10801 gttcctcgcc gccgtcatcg cccctgccgc tgcacgcagc aagtgtaacg gcgaccatga
    10861 tcaacaggac ggcgatgacg aaacggcggc tgtaggtaca catgcgcagt gtgatgaatt
    10921 ccatttcgtg atccttggcc ttcgacgggt ttgttggctc gctaaacgac ttgttcatgg
    10981 ccactgccgt attggcggta acggattcct gcctggaatc cggacaacga attgaatgct
    11041 ttcgccccta ctccacgcga tgcgccagca ccagcgcaca caccggctgt ccgcgacagg
    11101 gaacgctcgc gaacctggct tgcagcggtc ccagcgccat ggcgctttga cgccgcctcg
    11161 atgacgccgc ggcgtgcagc tcgcgttgca ggtgttccag gttgtcgtgc tgcagctcga
    11221 tgaaagttgc gcaaccggta cgctcgtcgc gcagccagcc gagtgccgcg tgcgcccagc
    11281 tacccgtttg tgactgcgcc atgtgtgact gcgccacgca caagcgctgg tcgtgtccgg
    11341 cggcctgcag tgccgcctcg tatgcagcat gattcgtcgg ccccgctccg gtgccggcga
    11401 tcagcctgac taccaccacg tcctcgaggt cgatttccag ctgatctcag cgcgatgcgc
    11461 tcgcgatcgc cggcgtggtc gtgtcgccat cgtcgaggac gctgccttga agaaccctgc
    11521 tgttttccgc ggtgtcttcg cgggtgattt ccacctgctc gatcctgtcg ctcgtgtcct
    11581 ggacgatggc cacttccgat ttgacgccgg ctttctcgat gaagtgtgtt tctctaacta
    11641 caaccacccg atcatcgtgc gacaattccc agccaggcac gagtcccact ggcaccaccc
    11701 agcaaggccg gccggaactt gtccgtacga caacaggccg gcggattcgc cggcgcacgg
    11761 gaggccagcg tcgcggattg tcataggcga aaacatcgcc tgttttcaca gtaagcgcga
    11821 tgccaagggg taccgccagc gcggtaaaga agtttcgacg tttcatggtc tctcctgcct
    11881 gttcgatttc gtccatgcgt gttggcgcca catcaccgct aacgggtatt catcgaaaaa
    11941 gcggacacgc ggctgcagga aaccgggtgc gcccggtttc ggccgcagat tcagacctgg
    12001 ttgtgatttg gcgagacggt catcggcgtt gccgtagtga ccggaaccag ctcgtcgctg
    12061 tgccgcagtg ggcgtgagat ccggcgaatc tccgcggtca acgcgatggc gagaatattc
    12121 cgccggcgta ttccgcaccg gagcgcagat agtcggcggc tggtacgcac atcctgccgg
    12181 tcttgtagat tttctcggtg tcgccgcccg cgacacgcac ggtgtacgtg ccgctggcgg
    12241 cggtgtacac cgtgacctcg atgtcttccc ggctcaagtt ccggatctcg aggcacccat
    12301 ccgcatcctt gccgaaggac gtgtaacggc aagcctgacc ctggcaggcg ccaacaccca
    12361 gtgccgatgc ggcgtacagg caacccagcg aggtcagcaa caatttcctg atcatggaaa
    12421 tctccgaata tccgtggttc agcgtacccg cgaccattcg aaagacggcc ccggatcggt
    12481 cttgcggccc ggggcaatgt cgctgtggcc tacgatccgg tcgcgcggga tggcataggc
    12541 ctctaacaac aagggaagca gcgtgcgcag cgtctcgtac tgcgccgctt catacggggc
    12601 ttcgtccgtc ccttcgcact cgatgccgat cgaatagtcg ttgcaggctt cgcgcccctg
    12661 ccacacggac ttgccggcgt gccacgcgcg atcgttgaac gagacgaact gttcgaccct
    12721 gccgtcgcgg cggaccagca gatgcgtcga gactctgagc gccgcgcgct cgcgaaattc
    12781 cgggtgcgcc gcggccggca ggttgcccgt gaacaggcgc gcgatccatg gcccaccgaa
    12841 ctcgccgggc ggcaggctga ttccatggag cacgagaagg tcgggaacga ctccggccgg
    12901 ccgttcatcg aaaaatggcg ataacacctg cttgactccg accatcaggc cggtactgac
    12961 atcgacgcgc atggctgttc ctcgcgccca tcttgccaca gcagcgtgcc acaatagccg
    13021 cccaagaaat ggcacgcacc gaaagcctca tgagttccgg caattccgac tgggtgacgc
    13081 gcgatctcgc gcatgtgtgg catccatgca cgcagatgaa ggaccacgag tccgtgccga
    13141 tgatcccgct gcgctccgga aagggcgtgt ggctcgagga cttcaccggc aaacgctacc
    13201 tcgatggcat cagctcctgg tgggtcaacc tgttcgggca cgccaacccg cgcatcaacg
    13261 ccgccgtgcg cgagcagtta gagcaactgg aacacgccat cttcgccggt ttcacgcatc
    13321 cgcccgcggt gcagctcgcc gaggaactgg tgcgcgtgac gcccgcgggc ctcaaccgtt
    13381 gtttttttgc cgacaacggc tcggcggccg tcgaagtcgc cgtgaagatg agctttcact
    13441 actggcgcaa caccggccac caacgcaaga cccgattcat cacgcttgcc aacagctacc
    13501 acggtgagac gctcggagcg ctcgcggtgg gcaacgtgga tctctacaag tccatctacc
    13561 ggccgctgct catggacgtg atcaccgtgc cctcgcccga cgccttcatg cgcgagcccg
    13621 gagtcaccgc cgccgagcat gcacggaaaa tgttcgcgca catggaagcg gccatcgcgc
    13681 agaaccacgg cgaagtggcg gcggtcatcg tcgagccgct ggtgcaatgc gccggcggca
    13741 tgcgcatgta cgacccggtt tatctgaaac tactgcgtga agcctgcgac cggcaccagg
    13801 tacatctgat tgccgatgaa atcgccgtcg gcttcgggcg caccggcacg atgttcgcct
    13861 gcgagcaggc cggcattcgg cccgactatc tatgtttgtc caagggcctc acgggtggtt
    13921 acctgccact ctcggccgta ctgaccaccg acggtatcta cgccgccttc tacgacgaat
    13981 acaccaggct caacgcgttc cttcattcac acagctacac gggcaatccg ctggcctgtg
    14041 ccgcggccaa cgccacgctc gccattttcc gcgaggagcc cgtgatcgaa cgcaatcgcg
    14101 cgacggcggc gcacatgctc actgcggtcg gacatctgca cgatcaccct catgtggcag
    14161 agatccgtca gtgcggcatg attctcgcca tcgagctcat gaaggatccc gccacccgaa
    14221 caccctggcc ctgggaacaa aggcgcggcc tgcgaattta ccggcacgcg ctcgaacacg
    14281 gcgcgctgat tcgcccgctc ggcactacca tctacttcat gccgccgtac gtcatcacgc
    14341 gcgaggagat cgacctgctg agccgcgtcg ccaccgaagg catcgatctc gcatgcgcgt
    14401 gacccgtatc ttcgtcgagc agaagctggt ctccggcaag ggcgtcggcc tccccgagca
    14461 ggccggcctg cacctcacgc gagtgctgcg cctcgaggcc ggcg
//
LOCUS       DASQJS010000449         7156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2179588, whole genome shotgun sequence.
ACCESSION   DASQJS010000449 DASQJS010000000
VERSION     DASQJS010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179588"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VEW08_17740"
     CDS             complement(<1..330)
                     /locus_tag="VEW08_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYJ42638.1"
                     /translation="MATMNLRVNGKQLSADVDPNTPLLWVLRDSLGLVGTKFGCGMAL
                     CGACTVLIDGNPVRSCSTPVSGVAGQSITTIEGLAPSPTRLHPLQQAWIDHDVPQCGY
                     CQAGQLMS"
     gene            580..1911
                     /locus_tag="VEW08_17745"
     CDS             580..1911
                     /locus_tag="VEW08_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42639.1"
                     /translation="MRSEAGSSFSTAQEMDLASRVLEATDVARLRDVVGSLLKRASDA
                     HGVRFDDATARSLKHRLLDTATRLGLPQINRLRLAAVRSPQVARGGALMSRATRLLGV
                     ELEGLSPEDKEFEVARRFVRLGSESVRQLRRVRRRPRSTAVATGTAMGRAQRALAPGL
                     SPPIETEPPSCACQGGCSCSSCGGAPADIPDTSNRSHAMHDIDHTKSEYEPELDELDE
                     LENDEMEFSDEYDSGELDEMETDAGEYYSGELDETGSPFSEVEEAELAQELLGLSSEG
                     EMDQFIGKLFKKAWRGAQGLTRKLGVAAPLGGILKGVAKKLLPVAAGAAGTFFGGPAG
                     AAIGSKIGSVVGNALEAEFEGSPEDREFEAAKTFVRVAGTAAQQAANASPSTPPVAAA
                     KNAVISASRDTVKRLRRRRNGASNYGGGYHGSGRRTGRWVRRGNRIILFGV"
     gene            1918..3231
                     /locus_tag="VEW08_17750"
     CDS             1918..3231
                     /locus_tag="VEW08_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42640.1"
                     /translation="MTPDARNLLSQEARALLTRLSLVKPLVLQETMVPAANVSDTASA
                     GIERLLVGGRRTVRALVRGFLGWLRGEGGARASDAQAQRRFSLVRLQFNTMLAQFDIF
                     ADAYSQRSEHDIGVWLAGLDVAAADALALPSYYRSPPLVCYLDRGIGAAIRRARTRLP
                     GGAPSPVGIVRVPRERMVGSGIASSLMHEVGHQAAALLGLVESLRGTLRGLQRGGRDA
                     DVWRLWDRWISEIVADFWSVARVGITSTLGLMGVVSLPPPFVFRVNLADPHPMPWIRV
                     RLSCAIGAALYPHPQWARLAELWRSLYPVDGLEAARRELISRLEDNMPAFVEMLVGHR
                     PPALRGASLREALAVDTRQPARLAQLFRDTQTRPGSLRKLSPSLAFAVVGQARANGEL
                     SPEGEAATISKLLRQWALADTLRSAESCAAAHHSFQAGRELAPAT"
     gene            3251..4771
                     /locus_tag="VEW08_17755"
     CDS             3251..4771
                     /locus_tag="VEW08_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42641.1"
                     /translation="MSVPREELPPPVAVRIDGLVRTSAQSRTVPTVKQAFWVAIRNRT
                     DALGFQRYSAFINRLLCEGKDGGQARCGDPDGKGDDGDRVRHGEYGEPSLAERRDEIF
                     ARPTLHGMDAYQVLLLATQAFVVFEAGVVVAAARLSNHSEFPGMDGLEGRMDRTVELK
                     DIKAELEEYLGASTGVGRGLPYLKQIVNTLVTVSSDGGVAQVPPWCRGILRNRFSCPS
                     MLELIWSYWHEEGMLVQSVNALSLRFQNRRIGDRDPLAQLEIDALRPLNNLLWGYIQD
                     EQNRLTLVRRAYEYDHHYGIKLVGKAVPTLRSADSRSKFIEGFHNLLYRCARFYQEDA
                     DTNVVADGFPLLNALKEVHLTLAEGAHNQFGDLPWTARVEMLIQQWLLARPEIRDFIR
                     GRVMVPYREDWMGQVDAMKKLQGWTDVTITHFHDLARYGEQMLLSIRYDDWSNVADQE
                     IAKAWVRFWRPEVQGYLHAYRSATGQDLTVEPVDSNSPSVHLRARLEDQRRRTPGG"
     gene            4780..5883
                     /locus_tag="VEW08_17760"
     CDS             4780..5883
                     /locus_tag="VEW08_17760"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYJ42642.1"
                     /translation="MPDFTSALYLGLRHPSAALKPWRALSSGRPAALAETAGAATVAA
                     GLARLTGCEAGTLLPSTLHLFLDLFASLPRRAHYFVDASLYPVARWGVERAVARGATA
                     SSFAHYDAGDLARQLARSASAARPPVAVIDGFCPACGVPAPLRDILAELRRYEGMLVV
                     DDTQALGIFGQSPGARCDALRVPEYGCGGGGMLRFADITGPDIVVASSLAKAFGVPVA
                     MLAGTKSFVTRFARSSATRVHCSPPSVAVVHAAERALAVNAERGDALRLRLAHLVRRF
                     RAGARGICALPRAGAFPVLTLGCEAARGAGAVRLHRALNEAGVRALLHAPRDPRISFL
                     ISAAHGESDVDDALAALQLCLSAGRRQRSGVVS"
     gene            5880..>7156
                     /locus_tag="VEW08_17765"
     CDS             5880..>7156
                     /locus_tag="VEW08_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYJ42643.1"
                     /translation="MRRDSDCSCHGQSQNYEALFDELQYEGTVTGPKPAGKSGVVVAP
                     GTTTPMVRFACLWKFPFKGTTMPAEHADLVTRVAHLILASRNFFNPITQVRLVGHTDD
                     RGSDKVNIDYGRKRAEDVRKRLIARLDALQPGLSQSVTIVADTGGESAPLRPHSAANA
                     AFNRRVEVYLPRNCQTFFAQMDLRSLPDTPILGIAAHPNITDKALRAEQVKLVVGALV
                     RRRDARAKMAADGKVMGAKPVDKAKTPKLHAAVQALSTMQLELFREYFGGKSIPLSIP
                     GFMTCFERFANGQLRSPHAAFAGAGVGEPEGGFFFLFAEFAMLCYDTRIDWRIWEQTL
                     PSLIAPQEIFMHVYHPTPMAPPPINAPLPAKGPVKTSLDAYSFRNFRPIGGSVVKGVG
                     QSDSQRINKLRTKYRGMSFLNLRKAIAQNMLRA"
BASE COUNT         1170 a         2381 c         2379 g         1226 t
ORIGIN      
        1 ggacatcagc tgacccgcct ggcagtagcc gcattgcggc acgtcatggt cgatccacgc
       61 ctgttgcagc ggatgcagcc tggtcggcga gggcgcgagg ccctcgatgg tggtgatgga
      121 ctgaccggcc acacctgaca ccggcgtcga acacgagcgc acgggattgc cgtcgatgag
      181 cacggtgcag gcgccgcaca gggccatgcc acagccgaat ttggttccaa ccagtccgag
      241 tgaatcgcgc agcacccaaa gcagaggtgt attcgggtcg acgtccgcgc tcagttgctt
      301 gccgttcaca cgcagattca tggtcgccat ggatgcttcc tcgatatttc ccgtttcttc
      361 ctatagcgga tttcgtgcct ggggggaatc cgaccgaagc gtgtttcgcc gcggcgacag
      421 gaaatcacgg acaacccgaa tcgcgcttcg gctccatcgc tgcggcgcgt ttcggcatgc
      481 gcaccgcgcg cggcgcctaa ctccctgctc cggctggaaa aacggcggcg tcagcgtgtg
      541 tggccggcat gctggcacgc gcgctgcatt ctcgaagtca tgcgatcaga agcaggaagc
      601 tcgttcagca ccgcacagga gatggatctc gcatcgcgag tcctggaagc gaccgacgtc
      661 gcgcgcctgc gggatgtggt cggttccctg ctgaagcgcg ccagcgatgc ccacggtgtc
      721 cggttcgacg atgcaaccgc ccgcagcctg aagcaccggc tgctcgatac cgcgacgcgg
      781 ctcggactac ctcaaatcaa ccgcctgcgg ctcgccgcgg tgcgctcacc ccaggttgcg
      841 cggggcgggg cattgatgtc gcgcgccaca cgtttgttag gtgtggagct cgagggactc
      901 agccccgagg acaaggagtt cgaagttgcg cgccgcttcg tccgcctggg tagtgagtcc
      961 gtgcgccagc tacgccgcgt gcgaagaagg ccgcgctcca ccgccgtggc caccgggacg
     1021 gcaatggggc gcgcccagcg tgcgctcgct cccggactct cgccgcccat cgaaacagaa
     1081 cccccttcct gcgcctgcca gggcggttgc tcctgcagca gctgcggcgg cgcgcctgcc
     1141 gacattcccg acacttcaaa caggagtcac gccatgcacg acatcgacca caccaaatcc
     1201 gagtacgagc ccgaactcga cgagctcgac gagctggaaa acgacgagat ggaattctcg
     1261 gacgaatacg attcgggcga gctcgacgaa atggaaaccg acgctggcga gtactacagc
     1321 ggcgagctcg acgagaccgg aagtcctttc agcgaagtcg aagaagccga actcgcgcag
     1381 gaattgctgg ggctctcgag cgaaggcgag atggaccagt tcatcggcaa gctgttcaag
     1441 aaagcctggc gcggcgcgca aggtctcacc aggaagctgg gtgtcgccgc tcctctcggc
     1501 ggcatcctca agggcgtggc gaagaagctg ctgcccgtgg ccgccggcgc tgctggtacg
     1561 ttctttggcg gcccggcggg cgcggccatc ggctcgaaga tcggctcggt ggtgggcaat
     1621 gcgctcgagg ccgagttcga aggcagcccg gaagatcgcg agttcgaggc ggcgaagacc
     1681 ttcgtgcgcg tggcgggcac ggccgcgcag caggcggcca acgcctcgcc gtccactccg
     1741 ccggtcgcgg ccgcgaagaa cgcggtgatc agcgcctcgc gtgacaccgt gaagcgcctg
     1801 cgccgccggc gcaacggcgc ctctaactac ggtggcggtt atcacggcag tggtcggcgc
     1861 accggccgct gggtgcgccg tggcaatcgc atcatcctgt tcggagtctg agctgcgatg
     1921 acgccggatg cccgcaacct gctgtcacag gaagcccgag cgttgctcac acggctgtcc
     1981 ctggtgaagc cgctggtgct ccaggagacg atggtgcccg cggccaatgt ctccgacacg
     2041 gcttcggcgg gcatagagcg cctgctcgtc ggcggtcgcc gcacggtgcg cgcgctggtg
     2101 cgcggcttcc ttggctggtt gcgcggcgag ggtggcgcgc gcgcatcgga cgcgcaggca
     2161 caacggcgat tctccctggt gcgcctgcag ttcaacacca tgctcgcgca gttcgacatc
     2221 tttgcggatg cgtactcaca gcgcagcgag cacgacatcg gcgtgtggct ggcgggcctg
     2281 gatgtcgcgg ccgccgatgc gctcgcgctg cctagctatt accgctcgcc gccgctggtg
     2341 tgttacctcg accgcggcat cggtgcggcg attcgtcgcg cgcgtacgcg tctgccgggt
     2401 ggcgcaccga gcccggtggg catcgtgcga gtcccgcgcg agcgcatggt gggcagcggc
     2461 attgcctcgt cgctgatgca cgaggtgggt caccaggccg ccgcgctgct cggactggtg
     2521 gaatcgttgc gcggcacgtt gcgcggcctg cagcgcggcg gcagggacgc cgacgtatgg
     2581 cggctctggg atcgctggat ctcggaaatc gtcgccgact tctggtcggt ggcgcgtgtc
     2641 ggcatcacct cgacgctggg cctgatgggc gtggtgagtc tgccgccgcc cttcgtgttc
     2701 cgcgtgaatc ttgccgatcc gcacccgatg ccgtggatcc gcgtgcggct gtcctgcgcc
     2761 atcggcgccg cgctgtatcc acacccccag tgggcgcggc tggctgaatt gtggcgctcg
     2821 ctctatccgg tcgacggact cgaggccgcg cggcgcgaac tgatctctcg cctcgaggac
     2881 aacatgcccg ccttcgtcga aatgctggtc ggccatcggc cacccgcact gcgcggtgcg
     2941 tcgctgcgcg aagcactggc agtggacacg cgccagcccg cgcggctcgc gcagctgttc
     3001 cgcgatacac aaacccggcc gggcagcctg agaaagctgt cgccgtccct cgccttcgcg
     3061 gtcgtgggcc aggcgcgcgc caacggcgag ctcagtcccg aaggcgaagc cgccaccatc
     3121 tccaaactac tgcgccagtg ggcgctggcc gacacgctgc gctcggccga atcctgcgcc
     3181 gcagcccatc actcattcca ggccggtcgc gagctcgcgc cggccacctg actacctgaa
     3241 ggaggacatc atgagcgttc ccagagaaga acttccaccc ccggtcgcgg tgcgcatcga
     3301 cggcctcgtt cgaacgtcgg cgcagtcgag aaccgtgccc accgtcaaac aggcattctg
     3361 ggttgcgatc cgcaatcgca ccgacgcgct gggattccag cgttattccg cgttcatcaa
     3421 ccggctgctc tgcgaaggca aggacggcgg gcaagcgcgg tgcggcgacc ctgacgggaa
     3481 aggcgacgat ggcgacagag tgcggcacgg cgagtacggt gagccctcgc tcgccgagcg
     3541 tcgtgacgaa attttcgcgc gaccgacgct gcatggcatg gatgcctacc aggtgctgct
     3601 gctcgccacg caggccttcg tggtcttcga agctggcgtt gtcgttgcgg ccgcacggct
     3661 cagcaatcac agcgaattcc ccggcatgga tggcctcgaa ggtcgaatgg accgcacggt
     3721 cgagctgaag gacatcaagg cggagctcga ggaatacctg ggtgcaagca ccggggtggg
     3781 ccgtggattg ccgtatctca agcagatcgt caacaccctg gtcacggtga gctccgatgg
     3841 cggcgtcgcc caggttccac cgtggtgccg cggcatcctg cgcaatcgct tcagctgccc
     3901 gagcatgctc gagctcatct ggtcttactg gcacgaggaa ggaatgctgg tgcagtcggt
     3961 caatgcgctg tcgctgcgtt tccagaatcg ccgcatcggc gaccgcgatc cgctggcgca
     4021 actcgagatc gacgcgctgc gcccgctgaa caacctgctg tggggttaca tccaggacga
     4081 gcagaaccgc ctcacgctgg tccgtcgcgc ctatgaatat gaccaccact acggcatcaa
     4141 gctggtcggc aaagcggtgc ccacgctgcg ttcggccgac agccgttcga agttcatcga
     4201 gggtttccac aacctgttgt atcgctgcgc gcggttctac caggaggacg ccgacaccaa
     4261 cgtcgttgcc gatggcttcc cgctgctcaa cgcgctcaag gaagtgcacc tgacgctcgc
     4321 cgagggagcg cacaaccagt tcggcgatct gccgtggacc gcgcgcgtcg agatgctcat
     4381 ccagcagtgg ttgctcgcac gtccggagat tcgcgacttc atccgcggcc gcgtgatggt
     4441 gccgtaccgc gaagactgga tgggccaggt cgatgccatg aaaaagctcc aggggtggac
     4501 cgacgtgacc atcacgcatt tccacgacct ggcgcggtac ggcgagcaga tgctgctgtc
     4561 cattcgctac gacgactgga gcaatgtcgc cgaccaggaa atcgccaagg cctgggtgcg
     4621 attctggaga ccggaggtgc agggctatct acatgcgtat cggtcggcca ccgggcagga
     4681 cctcaccgtc gagcctgtcg actcgaattc gccctcggtt cacctgcgtg cgcggctcga
     4741 agatcagcgt cgtcggactc cgggcgggta gtcacttgaa tgcccgactt cacctccgcg
     4801 ctgtacctgg gattgcgcca tccgagcgca gcactgaagc cgtggcgggc gctgagctcg
     4861 ggccggccgg cggcgctggc ggaaaccgcg ggcgcggcga ccgtggccgc ggggctcgcg
     4921 cggctcacgg gctgcgaggc cggcacgctg ctgccatcga cactgcacct gttcctggac
     4981 ctgttcgcat ccctgccgcg ccgtgcccac tatttcgtgg acgcgagtct ctacccggtg
     5041 gcgcgctggg gagtggaacg agccgtggct cgcggtgcga ccgcatccag ctttgcgcac
     5101 tacgacgccg gcgacctggc gcggcaactg gcgcgttctg catccgctgc tcgtccgccc
     5161 gtggccgtga tcgacggctt ctgtccggcc tgcggagtgc cggcgccgtt gcgcgacatc
     5221 ctcgcggaac tgcgtcgcta cgaggggatg ctggtcgtcg acgacacgca ggcgctgggc
     5281 attttcggcc agtcgccggg tgcccggtgc gatgcgctgc gtgtgccgga gtacggctgt
     5341 ggcggtggcg gcatgctgcg cttcgcggat atcaccgggc cggacatcgt ggtcgcgagt
     5401 tcgctggcaa aggcattcgg cgtgcccgtg gccatgctgg ccggcacgaa atccttcgtc
     5461 acgcgatttg cgcgctcgag cgccactcgc gtgcattgca gcccgccatc ggtggccgtc
     5521 gtgcacgcgg ctgagcgggc tctcgccgtg aacgccgaac gaggcgatgc ccttcggctg
     5581 cgcctcgcgc acctggtgcg gcgattccgc gcgggcgcgc gcgggatctg tgcgttgcca
     5641 cgcgcaggtg cgtttccggt gctcacgctg ggctgcgaag ccgcgcgcgg cgccggcgca
     5701 gtgcggctgc accgcgcgtt gaatgaagcg ggtgtgcgtg cgcttctgca tgcaccgcgc
     5761 gatccacgca tcagttttct catcagcgcg gcccatggcg aatccgacgt ggatgacgcc
     5821 ctggccgcac ttcagctttg tttgtcagcg ggccgccggc agcggtcggg agttgtgtca
     5881 tgagaaggga ttcggattgt tcttgccacg gccagtcgca gaattacgag gcgctcttcg
     5941 atgagctgca gtacgaaggc accgtgaccg gccccaagcc cgcgggcaaa tccggcgtcg
     6001 tggttgcgcc cggtaccact acgcccatgg ttcgtttcgc ctgcctgtgg aaatttccct
     6061 tcaagggcac gacgatgccg gcggagcacg cggacctggt cacgcgcgtc gcgcacctga
     6121 tcctcgcgag ccggaacttc ttcaacccca tcacccaggt ccggctggtg ggccataccg
     6181 atgatcgtgg cagcgacaag gtcaacatcg actacggccg caagcgcgcc gaggacgtac
     6241 gcaagcgatt gatcgcgcgg ctcgatgccc tgcagccggg attgagccag tcggtcacca
     6301 tcgtcgccga caccggcggc gagtccgcgc cattgcggcc gcacagcgcc gccaatgccg
     6361 ccttcaaccg ccgcgtcgag gtctatctac cgcgcaactg ccagacattc ttcgcgcaga
     6421 tggatctgcg ttccctgccg gacacgccca tcctcggcat tgccgcgcat cccaatatca
     6481 ccgacaaggc attgcgcgcc gagcaggtga agctggtggt gggggcgctg gtgcgcaggc
     6541 gtgacgcacg cgccaagatg gccgccgacg gcaaagtcat gggtgcgaag ccggtcgaca
     6601 aggccaagac tcccaaacta cacgccgcgg tgcaggccct ctccacgatg cagctcgagc
     6661 tgttccgcga atatttcggg ggcaagtcga tccctctcag tatccctggc ttcatgacct
     6721 gtttcgaacg cttcgccaat ggccagctgc gcagtccgca tgcggccttc gccggtgccg
     6781 gtgtgggcga gcccgaaggg ggcttcttct tcctgttcgc cgagttcgcg atgctctgct
     6841 acgacacgcg catcgactgg cgaatctggg agcagacgct gccctcgttg atcgcgccac
     6901 aggaaatctt catgcacgtc taccacccca cgcccatggc gccgccgccc atcaacgcgc
     6961 cattgccagc caaggggccg gtgaagacat cgctcgatgc ctacagcttc cgcaacttca
     7021 ggccgatcgg cggttcggtg gtcaagggtg tgggccaatc cgattcgcag cgcatcaaca
     7081 aactgcgcac caagtaccgt ggcatgagct tcctgaacct gcggaaggcc atcgcccaga
     7141 acatgcttcg cgcgca
//
LOCUS       DASQJS010000450         7557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2496368, whole genome shotgun sequence.
ACCESSION   DASQJS010000450 DASQJS010000000
VERSION     DASQJS010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496368"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VEW08_17770"
     CDS             <1..744
                     /locus_tag="VEW08_17770"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYJ42644.1"
                     /translation="RAVKPGSEEAKRLKAALIADGLWSQYLEVAIGPDAEIFTKAPTL
                     SSVGWGDWIGVRSDSAWNNPEPEIVLAVDSAGRILGATLGNDVNLRDIEGRSALLLGK
                     AKDNNASCAIGPFIRLFDGSFTLDDVRQAVVKLEITGEEGFRLEGQSSMSQISRDPTE
                     LVGQTIGKHHCYPDGFALFLGTMFAPIVDRGVAGQGFTHKNGDVVRVSTGKLGVLENK
                     VTTCDRAPPWSFGIADLMRNLMRRGLLNN"
     gene            759..2201
                     /locus_tag="VEW08_17775"
     CDS             759..2201
                     /locus_tag="VEW08_17775"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYJ42645.1"
                     /translation="MSDTKQLSHYIDGQWVAGASGSDSLNPSDTRDIVAKVPGGDKST
                     VNAAVAAAKAAFPAWANASPEVRSDLLDKVGSLIMARADDLGRLLSREEGKTLPEGKG
                     EVMRAARIFKFMAGEALRRHGVTVDSTRPGVAVETYREPLGVFGLITPWNFPIAIPAW
                     KSAPALAFGNTVVMKPASLTPATAHALTEIIHEAGFPKGVFNLILGGGAMGGALTEHP
                     DVAGISFTGGQSTGAQVAKAAMSHNARVQLEMGGKNPLVVLDDCDLDRAVQCALDGAF
                     FATGQRCTASSRIIVTQNIHDKFVEALAAKVKALKVGSALAADTQMGPLASQAQLDTT
                     LSYVDVATKQGGRRVVGGEVLTLATPGYYVAPALIADTTQDMRINLEEVFGPVASTVR
                     VKDYDEALAAANAGEFGLSAGIMTSSLKYARHFVRNVRAGMVMTNLPTAGVDYHVPFG
                     GSRKSSFGPREQGFAAVEFYTQIKTSYTWA"
     gene            2207..2959
                     /locus_tag="VEW08_17780"
     CDS             2207..2959
                     /locus_tag="VEW08_17780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYJ42646.1"
                     /translation="MTHAVYPSLANKTVVITGGGSGIGEVMVEGFSRQKSRVFFLDVA
                     DSESKALVARLGNGVQFINTDVTNIDALRAAFSEITTRAGPVSVLVNNAANDDRHTFE
                     EVTPTYWEQRLAVNLRHFFFGAQFVTPGMKAAGGGSIINLGSVSWHSAIPELSIYETA
                     KAGIEGMTRALARDLGEANIRVNCVVPGAIRTPRQMKLWHTPEEEAKILAQQCLKARV
                     DPVHVTAMVLFLASDDARMCTAHNYWVDAGYR"
     gene            2965..3837
                     /locus_tag="VEW08_17785"
     CDS             2965..3837
                     /locus_tag="VEW08_17785"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HYJ42647.1"
                     /translation="MSDIECVWPVGAILGEGPVWSAAEQALWFVDIKAPAIHRFDPAT
                     GAQRTWPAPARVGFCLPTTAGWMIAGLKTGLHKFNPETGNFALLHVVEPHTPSNRLND
                     GFVDSAGFLWFGSMDDIEEEPSGALYQLTDTGCLRRDPGYVVSNGPAESPDGRVLYHT
                     DTLAGIIYAFDRTREGSLSNKRVFARFGAGEGFPDGPIVDAEGCIWTGVFGGWGLRRF
                     SPQGELLSTLRLPCSAVTKAAFAGDDLRTLYITTAHVALDAEQRKQQPLAGGLFRVRV
                     DVPGLPQGIVSHGI"
     gene            3827..5623
                     /locus_tag="VEW08_17790"
     CDS             3827..5623
                     /locus_tag="VEW08_17790"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HYJ42648.1"
                     /translation="MESDDQTKQPRRRFRSRDWFDDPKHLDMVALYLERFMNYGLTAN
                     ELLSGKPIIGIAQTGGDLTPCNRVHLDTVKRVREGIRDAGGVPMEFPLHPIFENCRRP
                     TAALDRNLAYLGLVEILHGYPIDAVVLTTGCDKTTPAQVMAASTVDIPAIVLSGGPML
                     DGWHDGDLVGSGTVIWRSRRKLAAGQITEKQFLDAAAASAPSLGHCNTMGTASTMNAV
                     AEVLGLSLPGCAAIPAPYRERGQISYETGRRIVELAYDDLRPSKILTRQSFLNAITAI
                     AAIGGSTNAQPHLVAMARHAGIEITPRDWMEYGRDIPLLVNMQPAGKYLGERFHRAGG
                     VPAVLNELLRVGKLDGQALTVTGRTLGENVGAYINGRTAVDREVITTYEKPVQEKAGF
                     LVLSGNLFDFAIMKTSVISPEFRKRYLGQGDSFECRAVVFDGSTDYHERINDPALGID
                     EHTILVIRGSGPLGWPGSAEVVNMQPPDALLKRGITSLPTLGDGRQSGTSDSPSILNC
                     SPESAAGGGLAWLRTGDMIRIDLAAGTCNMLVADDEIERRKGVGLPPVPESATPWQEL
                     YRSTVGQLDSGAVMEMAVKYRGVARKTPRHNH"
     gene            5646..7124
                     /locus_tag="VEW08_17795"
     CDS             5646..7124
                     /locus_tag="VEW08_17795"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HYJ42649.1"
                     /translation="MSPPRLTEASLHSVRSGTILPTYDRGATRIGIVHIGPGAFHRAH
                     QAYYVDTLLHSDKRWAISALSLKSTGLRDALLPQQGLYTLVELGAAPRARVIGAIREM
                     LVGSTDSEAAFARLTARDTRIVSLTVTEKGYCLDARNQLDLANADIAHDLANPPSSKE
                     LGPRSTIGWLVEGLRRRRAQGVPPYAVLSCDNLPDNGVVLKRALAAYARTSDADLARW
                     IEAEVTCPRTMVDSITPATDDALRARAEKITGVLDAWPIQREPFTQWVVEDVPVMRDA
                     DWKSVGVTLAKDVGVYDRAKLRLLNGPHSSLAYLGLLRGHDSVADAMRDEQLAQFVEL
                     LMTEDLSPSLGAEPGLDVEHYISAVLARFRNPGIRHLLSQIAWDGSKKLPVRLVVTIG
                     EAMRAGRPIHRLLMPVAAWMRFIVRQAKAGVPIVDPDAARLASIGSACTGDARADLPR
                     FAVCESVLPPALMSEERFRKELETAYDRLAAPQAALTVELSS"
     gene            7121..7513
                     /locus_tag="VEW08_17800"
     CDS             7121..7513
                     /locus_tag="VEW08_17800"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HYJ42650.1"
                     /translation="MSTRRTFIAAAATVAATNAGAPPAVNAIPKLVHHVFFWLKNPSS
                     QEDLAKLLAGLRTLAAIETVRATHFGVPASTEKRDVVDNSFSASEILFFDDTAGQKVY
                     QDHPIHKQFVADCSHLWAKVVVYDAIAS"
BASE COUNT         1395 a         2483 c         2346 g         1333 t
ORIGIN      
        1 cgcgccgtga aaccgggttc cgaggaagcg aagcggctca aggcggcgct cattgccgat
       61 ggactctggt cgcagtatct cgaggtcgcg atcggaccgg acgcggagat cttcaccaag
      121 gcgccgacgc tttcatccgt cggctggggc gactggatag gcgtgcgctc cgactcggcg
      181 tggaacaacc cggagccgga gatcgtgctg gcggtagata gtgccggacg catcctgggc
      241 gcgacgctcg gcaacgacgt caaccttcgc gacatcgaag gacgcagcgc gttgctcctg
      301 ggcaaggcga aagacaacaa cgcctcgtgc gccatcggcc cgttcatccg actgttcgat
      361 ggcagcttca cgctcgacga cgtgcggcag gccgtcgtga agctggaaat cacgggcgag
      421 gaaggctttc gcctggaagg ccagagctcc atgtcgcaga tcagccgcga ccccaccgag
      481 ctcgtcggcc agaccatcgg gaagcatcac tgctacccgg acggcttcgc actttttctc
      541 ggcaccatgt tcgcccccat cgtcgatcgc ggcgtagcgg ggcaaggttt tacgcacaag
      601 aacggcgatg tcgtccgggt gtccaccggg aagctcggtg tgttagaaaa caaggtaacc
      661 acctgtgacc gggccccacc gtggtcattc ggaatcgcgg atctgatgcg caatttgatg
      721 cgtcgcggcc tgctgaacaa ttgaatggaa cttaaatcgt gagcgacaca aaacagcttt
      781 ctcattacat cgacggccaa tgggtcgccg gcgcgtcagg ctcggacagt ctcaatccct
      841 cggacacgcg cgacatcgtc gccaaggttc ccggcggcga caaatcgacg gtgaatgccg
      901 ccgtagccgc ggccaaagcc gcgtttcccg cgtgggcgaa cgccagcccc gaagtgcgct
      961 cggacctgct cgacaaggtg ggttcgctga tcatggcgcg cgccgatgac ctcggccgcc
     1021 tgctctcgcg tgaagaaggc aagaccctgc ccgagggcaa gggcgaagtc atgcgcgcgg
     1081 cgcgtatctt caaattcatg gcgggcgaag cgcttcgccg ccacggcgtc accgtcgatt
     1141 cgacgcgacc tggcgttgcc gtcgaaacgt atcgcgaacc cctcggcgtc ttcggactga
     1201 taacgccgtg gaactttccg atcgcaattc cggcatggaa gagcgcgcca gcactcgcct
     1261 tcggcaacac cgtggtcatg aaacccgctt cgttgacgcc ggccacagcg cacgcgctga
     1321 ccgaaatcat tcacgaagcc ggcttcccaa aaggcgtgtt caacctgatt ctcggcggcg
     1381 gcgccatggg cggcgcgctc accgagcatc cggatgtcgc cggaatttcg ttcaccggcg
     1441 gccagagcac cggcgcgcag gtcgccaagg cagccatgtc gcacaacgca cgcgtgcagc
     1501 tcgaaatggg tggcaagaac ccgctggtgg tgctcgacga ttgtgatctc gatcgcgcgg
     1561 tgcaatgcgc gctcgatggc gcgtttttcg cgaccggcca gcgctgcacg gcctcgagcc
     1621 gcatcatcgt cacgcagaac atccacgaca agttcgtcga agcgctcgcc gccaaagtga
     1681 aagcactgaa agtcggcagc gcgctcgccg ccgacacgca gatgggcccg ctggcgagcc
     1741 aggcgcagct cgacaccact ctctcttatg tggatgtggc gaccaaacaa ggcggccgcc
     1801 gcgtggtcgg cggcgaagtg ctcacgcttg cgactcctgg ctactacgtg gctcccgccc
     1861 tcatcgcgga cactacccag gacatgcgca tcaacctcga agaagtcttc ggcccggtcg
     1921 cctcgactgt gcgcgtaaag gattacgacg aagctctcgc cgccgcgaat gcgggcgaat
     1981 tcggcctgtc agccggcatc atgaccagct cgctcaagta cgcgcggcat ttcgtgcgca
     2041 acgtgcgcgc cggcatggtc atgaccaacc ttcccaccgc gggcgtggac tatcacgtgc
     2101 cgtttggtgg ctcgcgaaaa tcgagctttg gtccgcgcga acagggtttc gcggccgtgg
     2161 agttctacac gcagatcaag acaagttata cctgggccta gacaacatga cgcacgcggt
     2221 ttatccgtcc ctcgccaaca agaccgtcgt catcacgggc ggcggttccg gcatcggcga
     2281 ggtgatggtg gaaggcttct cgcgccagaa gtcgcgggtc ttctttctag atgtcgccga
     2341 cagcgagtcg aaagcgctgg tggcgcgcct ggggaacggc gtccagttca tcaataccga
     2401 cgtgaccaac atcgacgcgt tgcgcgctgc gttttccgaa atcaccacca gggccggtcc
     2461 cgtctcggtg ctggtgaaca atgccgccaa tgatgatcgg cacaccttcg aagaggtgac
     2521 cccgacgtac tgggaacagc ggctggcggt gaacctgcgc cacttcttct tcggcgcgca
     2581 attcgtcacc cccggcatga aggcggcggg cggcggctcg atcatcaacc tgggctcggt
     2641 gtcctggcac tcggcgattc ccgagctctc gatctacgaa acggcgaagg ccggcatcga
     2701 aggtatgaca cgagcgctgg cccgcgacct gggtgaggcc aacatccgcg taaattgcgt
     2761 ggtcccgggt gccatccgga ctccgcgcca aatgaagctc tggcacacgc cggaggaaga
     2821 ggccaagatc ctcgcgcagc agtgcctcaa ggcgcgcgtc gatccggtcc atgtcaccgc
     2881 aatggtgctg ttcctggcat ccgacgacgc ccgtatgtgc actgcgcaca actactgggt
     2941 ggacgcgggt taccgataag agtcatgtcc gatatcgagt gcgtctggcc agttggcgcg
     3001 atcctcggag agggcccggt ctggtcggcc gccgagcagg cgctgtggtt cgtggacatc
     3061 aaggcgcccg ccatccatcg attcgatcct gcgaccggcg cccagcgcac ctggcccgcg
     3121 ccggcacgcg tcggcttctg tctgcccacc accgctggct ggatgatcgc cgggctcaag
     3181 accgggcttc acaagttcaa cccggagact ggcaatttcg cgttgctgca cgtggtcgag
     3241 ccgcacacac ccagtaaccg gctcaacgac ggctttgtcg attccgccgg cttcctgtgg
     3301 ttcggatcta tggacgatat cgaggaagaa ccgagcggcg cgctgtacca gctcactgac
     3361 acgggctgcc tgaggcgcga tccgggctac gtggtgagca atggtccggc ggaaagtccc
     3421 gacggccgcg tgctgtatca caccgatacg ctcgccggca tcatctatgc attcgatcgc
     3481 acgcgtgaag gttcgttgtc taacaaacgc gtgttcgcgc gtttcggcgc aggcgaaggc
     3541 tttcccgacg gacccattgt cgatgccgaa ggttgcatct ggaccggggt gttcggcggc
     3601 tggggtctca ggcgtttttc accccagggt gaattgctgt cgaccctgcg actgccctgc
     3661 tcagccgtga ccaaggcagc cttcgccggc gatgacctga ggacgctcta catcaccacc
     3721 gcgcatgtcg ctctcgatgc ggaacaacgc aagcagcagc cgctcgcggg cggcctgttc
     3781 cgggttcgcg tcgatgtgcc cgggctgcca cagggaatcg tgtcgcatgg aatctgacga
     3841 tcaaaccaaa caacctcgcc gccgcttccg ttcgcgcgac tggttcgacg atccgaaaca
     3901 cctggacatg gtggcgctgt atctcgagcg cttcatgaac tacggcctca cggccaatga
     3961 attgctgtcg ggcaagccca tcatcggcat cgcgcagacc ggtggcgatc tcacgccgtg
     4021 caatcgcgtg catctcgaca cggtgaagcg cgtgcgcgaa ggcatccgcg atgccggcgg
     4081 cgtgcccatg gaattcccgt tgcaccccat tttcgagaat tgccgccggc ccacggcggc
     4141 gctcgatcgc aacctcgcgt acctcgggct cgtcgagatc ctgcacggat atccgatcga
     4201 tgcagtggtt ctcaccaccg gctgcgacaa gaccaccccg gcgcaagtga tggctgcatc
     4261 gaccgtcgac attccggcca tcgtgctgtc aggtggaccg atgctcgacg gctggcacga
     4321 tggcgatctc gtcggctccg gcaccgtcat atggcgttcg cgccgcaagc tcgccgccgg
     4381 gcaaatcacc gagaagcagt ttctcgatgc ggccgctgcg tcagcgcctt cactcggcca
     4441 ctgcaacacg atgggcacgg cttccacgat gaacgccgtc gctgaagtgc tcggactctc
     4501 cctgcccggt tgcgctgcca tacccgcgcc ctatcgtgag cgcggccaga tctcgtacga
     4561 aaccggacgc cggatcgtcg aactcgcata cgatgatttg cggccgtcga agatcctcac
     4621 gcgccagtca ttcctgaatg ccatcaccgc gatcgcggcg atcggtggct cgacgaacgc
     4681 gcagccgcac ctggtcgcca tggcgcgcca cgccggcatc gagatcacgc cgcgtgactg
     4741 gatggagtac gggcgcgaca tcccgctgct ggtaaacatg cagccggcgg gcaaatacct
     4801 gggcgaacgc ttccatcgtg caggcggtgt gccggcggta ctaaacgagt tgctgcgcgt
     4861 cggcaagctc gacggccagg cgctcaccgt caccgggcgc acgctcggcg agaacgtcgg
     4921 cgcgtacatc aacggacgta cagcggtcga ccgcgaagtc atcacgactt acgaaaaacc
     4981 ggtgcaggaa aaggccggtt tcctcgtgct ttcgggaaat ctcttcgatt tcgccatcat
     5041 gaagaccagc gtgatttccc cggagtttcg caagcgttat ctcggccagg gcgacagctt
     5101 cgagtgccgc gccgtcgtat tcgacgggtc caccgattac cacgagcgaa tcaacgatcc
     5161 tgcgctcggc atcgacgagc acaccatcct cgtgatccgc ggctcaggcc ccttgggctg
     5221 gcccgggtcc gccgaggtgg tcaacatgca gccgccggat gcactgctga agcgtggtat
     5281 cacgagtctg ccaacgctgg gcgacggccg gcagtcggga acctcggaca gcccctccat
     5341 tctcaattgt tctcccgaaa gcgccgcggg tggcggactc gcatggctgc ggaccgggga
     5401 catgatccgc atcgacctcg ccgccggcac ctgtaacatg ctcgtggccg acgatgagat
     5461 agagcgacgc aagggcgtgg gcctgccgcc cgtacccgaa agcgccacgc cctggcagga
     5521 gctgtatcgc tccacggtcg ggcaactcga cagcggcgcc gtcatggaaa tggcggtgaa
     5581 ataccgcggt gtcgcacgca agacgccgcg ccacaatcat tgaatagtta gtcaacgagg
     5641 ttctgatgag tccgccgcgc ctcaccgagg cgagcctgca ttccgtgcgt tcgggcacga
     5701 ttctccccac ctatgaccgc ggtgcgacgc gcatcggcat cgtgcacatc ggcccgggcg
     5761 cttttcatcg cgcgcaccag gcgtactacg tcgacacgct gctgcactcc gacaaacgat
     5821 gggcaatttc cgcgctgtcg ctcaagagca ccggtctgcg cgacgccctg ctgccccagc
     5881 aagggctgta cacgctggtg gaactcggag ccgcaccgcg cgcacgcgtc atcggcgcga
     5941 tccgcgagat gctggtgggc tcgaccgatt cggaagccgc cttcgcacgc ctgacggccc
     6001 gggatacgcg catcgtctct ctgacggtca ccgaaaaggg ttactgcctc gatgcgcgca
     6061 atcagctcga cctggccaac gccgacatcg cgcacgatct cgcgaacccg ccatcttcga
     6121 aggaacttgg accgcgaagc accattggct ggctcgttga aggtcttcgg cgccggcgcg
     6181 cccagggagt cccgccatac gccgtattga gctgtgacaa cctcccggac aatggcgtgg
     6241 tcctgaagcg cgcgctggcc gcctacgccc gcacttccga cgcagacctc gcacgctgga
     6301 tcgaagcgga agtcacctgt ccgcggacga tggtcgacag catcacgcca gccacggatg
     6361 acgctctgcg ggcccgcgcc gaaaaaatca ccggcgtgct cgatgcctgg cccattcaac
     6421 gcgagccgtt cacgcaatgg gtggtcgaag acgtgccggt catgcgggac gccgactgga
     6481 agtccgtggg cgtcacgctg gcgaaagacg tgggcgtcta cgaccgggcc aagctgcggc
     6541 tgctgaacgg cccgcattca tcgctcgcct acctcggcct gctgcgtgga cacgactcgg
     6601 ttgccgatgc gatgcgcgac gagcagctgg cgcaattcgt cgagctgctc atgaccgaag
     6661 acctttctcc aagccttgga gcggaacccg ggctcgatgt cgagcactac atctccgccg
     6721 tgctcgcccg ctttcgcaac cctggcattc ggcatttgtt gagccagatc gcctgggacg
     6781 gctccaagaa gttgcccgtc cggctcgtcg tgacgatcgg cgaagcgatg cgcgcaggcc
     6841 gtcccatcca ccggctgctg atgcctgtcg ccgcgtggat gcgcttcatc gtgcgtcagg
     6901 ccaaagccgg cgttcccata gtcgatcctg acgccgcacg cctcgcgagc attggctcgg
     6961 cctgcaccgg cgacgcccgt gctgacctgc cacggtttgc agtctgcgaa tcggtgctgc
     7021 cgccagccct gatgagcgag gagcgattcc gcaaggagct cgagaccgcg tacgacaggc
     7081 ttgccgcgcc gcaggcggcg cttaccgtgg agttatcctc atgagcacgc gaagaacttt
     7141 cattgcagcg gccgccaccg tggctgcgac gaatgctgga gcaccccccg ccgtgaacgc
     7201 cattccgaag ctggtccatc acgtgttctt ctggttgaag aatccgtcgt cgcaggaaga
     7261 tcttgcgaag ttgttagcgg gcctgcgcac gctcgccgcg atcgagaccg tgcgcgccac
     7321 gcatttcggc gtcccggcga gcacggagaa gcgcgacgtg gtggataaca gcttcagtgc
     7381 gtcggagatt ctgtttttcg acgatactgc cgggcagaag gtttaccagg accatccgat
     7441 ccataagcag ttcgtcgccg actgctcgca cttgtgggcg aaggtcgtcg tttacgacgc
     7501 gatcgccagc tgaatcgtca gctttgacgt cacttcagga ccgtcaaact caacggg
//
LOCUS       DASQJS010000451        16785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_925233, whole genome shotgun sequence.
ACCESSION   DASQJS010000451 DASQJS010000000
VERSION     DASQJS010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16785
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925233"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /gene="pyrB"
                     /locus_tag="VEW08_17805"
     CDS             complement(<1..230)
                     /gene="pyrB"
                     /locus_tag="VEW08_17805"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HYJ42651.1"
                     /translation="MTQQNRPNGTLRHLLTLEGMSRQQMEALLTRAQKFVKPAGEPPA
                     HKRTLAGITVANMFTEPSTRTRVSFELAAKRLG"
     gene            complement(227..679)
                     /gene="ruvX"
                     /locus_tag="VEW08_17810"
     CDS             complement(227..679)
                     /gene="ruvX"
                     /locus_tag="VEW08_17810"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HYJ42652.1"
                     /translation="MPDLVPNDAKIIVALDFGTRRIGIASGDTLTRAAHPRSTITNGP
                     HGPDWRALDRALADTRPARIAVGEPYNADGSASPLTEAARRFASEVATRSGLPVDLVD
                     ERWSSQDAEERLRAQRESGERKRRVTREAVDAAAAAIILERWFATQET"
     gene            complement(672..1235)
                     /locus_tag="VEW08_17815"
     CDS             complement(672..1235)
                     /locus_tag="VEW08_17815"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HYJ42653.1"
                     /translation="MSGVTSLSNHLLIAMPQLADPNFSQTVSLICEHGEKGALGIVLN
                     RPLTMTLSEVFEQMKIEPTDKRAADLPVLRGGPVHQDRGFVLHRPGGEWDSTHKISES
                     IQVTTSRDVLAAMATGTGPEKAFIALGYAGWEAGQLEKEMLDNSWLSVPADESVIFDL
                     PYEERWLATLRLLGVEPGQLTSFSGHA"
     gene            complement(1246..2157)
                     /locus_tag="VEW08_17820"
     CDS             complement(1246..2157)
                     /locus_tag="VEW08_17820"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYJ42654.1"
                     /translation="MARAMQLREGIAPTRDRLTTTVFMAALVHGVIIVGVTFGGVRHE
                     KSGAPGLEVLIVSDDVPEARHNENATYLAQRTQLGSGNTVNPGEVARAPRAAAEHAAR
                     NGRNDGTVLADDAGALLATADDRALTTTAPMPEILYFAPPSSMTEMTEVPVQTDGNSE
                     QDKLGIDDDEEQQVRGPQRDELWVTPDTREALVAPYLVAWRLKIERLGTMNFPQAAWR
                     APGTRNPDVEVSILADGTLESATIVRSSGSAKLDQAVVDILKLASPFDPFPRELAEKH
                     RVLRFKYGWEFQNPGRHGTVTAPGNSR"
     gene            complement(2159..3127)
                     /gene="gshB"
                     /locus_tag="VEW08_17825"
     CDS             complement(2159..3127)
                     /gene="gshB"
                     /locus_tag="VEW08_17825"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HYJ42655.1"
                     /translation="MPKTTSLAVVMDPIAKIKFAKDSTLAMLLAAASRGWQLTYFEQS
                     DLFLRDGVALGRGRDLRVFADPAKWFELGEPTVRRLGEFDCVFMRKDPPFDAEYIYTT
                     YILERAEIQGAFVANRPQGLRDMNEKVFTAWFPQCCAPTLITRSAADITAFAAEHGKI
                     VVKPLDGMGGKSIFVTEKGDKNLPVIIETLTDYGQRFAIAQRYVPDISTTGDSRILLI
                     DGQPVPYALARIPLPSDNRGNLAAGATGVGRELNERDRWLAAEIGPRVAELGMVFVGI
                     DVIGGFVTEINVTSPTGIRELDKQFGLNIGDMVIAAIERRAGAKKG"
     gene            3315..3680
                     /gene="pilG"
                     /locus_tag="VEW08_17830"
     CDS             3315..3680
                     /gene="pilG"
                     /locus_tag="VEW08_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HYJ42656.1"
                     /translation="MKVLVIDDSKTIRKTAETLLAKEGCEVFTAVDGFDALAKVADYT
                     PDIVFVDIMMPRLDGYQTCSLIKHNKVFKSIPVIMLSSKDGLFDRARGRIVGSEYYLT
                     KPFTKDELLSAIETHVAAR"
     gene            3683..4048
                     /locus_tag="VEW08_17835"
     CDS             3683..4048
                     /locus_tag="VEW08_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ42657.1"
                     /translation="MALILIVDDSPTEVHVMKKALEKSGYQTATAQDGAEGVRMAREM
                     TPDLIFMDVVMPGVNGYQATRTLANDPKTRTIPIIMVTSKGQETDKIWGLRQGAVDYM
                     VKPVSPDQLVAKAQATLAA"
     gene            4073..4636
                     /locus_tag="VEW08_17840"
     CDS             4073..4636
                     /locus_tag="VEW08_17840"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HYJ42658.1"
                     /translation="MNEPATLKSLRDRPFELLAELERRGRAVTAAAASENPGAREWVG
                     VAFRMAGELYLAAREETREVLAVPPGLTRVPGAKPWVKGIANVRGQLLPILDLRQYLG
                     SGLTPNSRNVRVIVVNHREVPAGLVVDEVLGFRRFSESEFSGDAPPTVVRCERYLAGA
                     FRRGPEQWPVLSLRQLVESPSFQEAAA"
     gene            4633..6024
                     /locus_tag="VEW08_17845"
     CDS             4633..6024
                     /locus_tag="VEW08_17845"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYJ42659.1"
                     /translation="MSTEVGGGSRTLKSINTLITTTLVCVVLGALAWVAAYLLSLTIL
                     YAVAGVLAIVAAVPLFLMFGRVRAVEADIGHREALSLAQRKETERNQQAILRLLDELA
                     PLADGDLTVQATVTEDITGTIADSINYAIGVLRDLVSTINQSAIQLDGATRQTQALSA
                     HLAKASSAQSKQIAGATESASNMASSTEAISGNSERASDVARHSVDVAHKGGDAVRRT
                     IDGMNAIRETIQETSKRIKRLGESSQEIGNIVELINDIAEQTNILALNASIQASMAGD
                     AGRGFAVVADEVQRLAERAAAATRQIETLVRAIQADTNEAVVSMERSTTDVVGGALLA
                     ENAGAALEEIEQVSNQIASLVNNIFSSSRQQVSEAQKIARNMQVLKEISTQTAESTTA
                     TSQSISKLAQLSAAMRQSASGFRLPGAPVPRQVAAAGAAIAAAAAATTGISPAQPSTG
                     ATTRTRAISLAAS"
     gene            6049..11790
                     /locus_tag="VEW08_17850"
     CDS             6049..11790
                     /locus_tag="VEW08_17850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HYJ42660.1"
                     /translation="MPEVALQTLDLVGRELNNTLGEARTALETFVEQPENVVLLQRCV
                     SDLHQVQGVLRLLEIFGAALLAEEMEQVTLYLQTPASQKNQAETLDALMRAMVQLPSY
                     VERVLAGGRDLALVLLPLLNDLRAVRGSPLLSEGTLLLLNLKSDQQAAPVTPNANEPA
                     LTVAQWARRLRTRFQLGLIGWIRGERPEQNLEILETVAHQIEQVATRQSVFQLWWVVG
                     AIVEALREGGLETGVSVKRLLGLADREILQLYTQGEGRYSQNPPVELLNNLLYYAARA
                     TSNGPKVTAVRASFRLNELLNVDDSVEQERENLSAPSVKLMRTVAAAIREDLGKVKDV
                     LDIFVRRGGQPAELEPQVGMLRKIADTLGVLGLGELRQLVIEETNRLEAMAAGKTASD
                     HAALVHIAATLINVEDRLDRDLIGLIVPKTQVQQEEAGSVDADFQQVQAAVLRECSVN
                     LVRVKEAIASNVAGTLDVGALDAWPGLVAGIKAGLLMLGKSRAAEIVDGIARNLKELL
                     QPGGTAVPPNYLDRLADAVVSLEYYMETLQAGRTDPWYMLDNAQTCLQALSQMPKRVV
                     PTVPPLGPEAYAATVLLADAGATARAHALAAGNPPTIQGVTPTLHTSGQPPIGAKPIN
                     IDPELLTLYIEEAREEVARIAKLFPAWEQNPLETDALAGVRRAFHTLKGSGRMVGATD
                     ISEFAWSIENLLNKIIENTLQRSPSILAVIRDASAVAGELVTALEAGQGAPAKVQSII
                     DRAHALAANRPSASSQTATMEILERTLETRRDDVVSATNRVPTLQPPAEPAPAVSESS
                     IGESTMEVVEDWSAAAQESGENIVLSAPEEEPSGDMQLRDIYSRETQVNIAAVTQFIE
                     GERSRNAPHVVSEEAYRACHTLSGSSRMAEARHGIRLTDPLEHWVRKSFDSGVGLEAD
                     ELNLLADCMNAMQTVASHLDESTGFFQSHSTLLTRIDQATENLEQRIVAAARAAELAN
                     APAPAPPAASPVSVAPAVAAPPASFPPVAATPPVAPTPPAALVEDVLTDFDPDIASIF
                     TDEAVELIEQAQSALAQWNENRSSVDGLDALKRPLHTLKGGARMAGLIPMGDLSHELE
                     TLFMQIDSKVVAPDDRAFGLAQTSLDELARMRESVSSGKGVPTANALIAKIHALVSGT
                     PAAKDPAPPPPAPPVAPAAMAPPPPPAPAPPVAAPAPVMAVPPPPPIVAPAPAPAPVP
                     RPAPVPTMVPTISAVPAPKASYVPPPPRAEPAPEPPVLAAVWAPSSEPPSIVTRAAAR
                     EPMSAGFLRDGANKDAEGAGDGPLLPPGAVPPGREPTAPSDRPELARVDAELLDQLLN
                     NSGEASIARARLEQQIGSIDFNVGELSRTVTRLKEQLRKLELETEAQILHRFEEEKGQ
                     KSDFDPLELDRYSSIQQFSRGLAETANDVGSIQQLLETLTKDTQNLLTQQARTITELQ
                     NGLMRTRMVPFQRHVQRLARIVRQAATDTHKKAELQIEGASGELDRQVLERMMPPFEH
                     MLRNAVAHGIEKPEDRKKAGKSESGTITIALHREGSEVVVEVSDDGAGMNLKAIRDKG
                     ISVGLVRADQQLSDEDIIQLVLEPGFSTAGAVSTLSGRGVGMDVVASEIKKLGGALHM
                     ETKQGEGSRFTIRLPLTLAISHALILRANDELYALPLPTVEGVVRLSRTEVEAHLGPE
                     APAFEYGGQKYKFQHLAFYVGREPGPLPDGDVTVPVILVRAGEHSTGLVTDELVGSRE
                     IVVKSVGPQIAAIRGISGATILGDGRIVIILDIGALVRTDFRARTEPVMPREKTDTRS
                     VALVVDDSITVRRVTQRLLERNGMRVLTARDGVDAMEMLQEHTPDIILLDIEMPRMDG
                     YEVATQVRADPRLSGIPIIMITSRVGEKHRARAIEIGVDDYLGKPYQEAQLLEAIEPL
                     IARRREQHMREAN"
     gene            11807..12283
                     /locus_tag="VEW08_17855"
     CDS             11807..12283
                     /locus_tag="VEW08_17855"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HYJ42661.1"
                     /translation="MAERITEIYSLLIPLLDGRLIIPRSCVAEVVGFQPPSEMPGAPP
                     WYLGTFAWGSRQIPLVSFEGTCGQTIPPLTGRTRVVVLFALGNQVEGGYYGIVSQGFP
                     QLVRVSADILRPDPTKTFPERTPIISQVRMINEAPWIPDLERIEEMISEETSVLVE"
     gene            12430..14463
                     /locus_tag="VEW08_17860"
     CDS             12430..14463
                     /locus_tag="VEW08_17860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HYJ42662.1"
                     /translation="MAETMAHHYRPDVDGLRALAVVAVVAFHAAPKLLPAGFLGVDVF
                     FVISGFLISGLVLDQLRAGKFTMSGFYLRRARRILPALLLMLVISSLMALAILMPDEM
                     QDFAKLLIASVSFVPNLALLSETGYFDVGAHMKPLLHMWSLGVEEQFYFIWPFFLMLM
                     ARRDRPGQTLLMIGVLAVLSFLVHLFIFRDSPESSFYLPFTRFWELMIGAALAAIPAG
                     ARPFGESRPLHNAISIVGLLLMLGAMMWRGGDELSPFVGVAPTLGAAMFIAAGPLALP
                     NRSAFSWRPVVYVGLISYPLYLWHWPLLSLLRIADVEDHATQRMLKILMVVLAVVLAI
                     LTYHLVERPLRRRNDLRRIGAYLGLLLVAVGAWGVVIIVSDGFPNRTTLRENPIPWPA
                     ELRNSPRCMEAEGFAPDLREEALCTRSDYDRTPRIVIMGDSHANAISPGVFAAYPEDS
                     VLAIGASACLYLRRTDFWHAAAPGKRGTCPGLIDTAYLTITPDTRLVILAARYTMYVG
                     SAAEAAEMFDFPGTQYFSSPDFPGAGKIEAFERSLSRDLSLLLESDREVVLVLQVPEL
                     DFLPKRCLHARPFQRLLPKPEVRCSVPRARAERRQASYRAAVSRVARALNNPRLHVVD
                     PMDVLCDAKECHAMIGGMLMYRDDDHLSIGGSKYVWEKIRPRDLRAFAPPAAR"
     gene            complement(14476..14868)
                     /locus_tag="VEW08_17865"
     CDS             complement(14476..14868)
                     /locus_tag="VEW08_17865"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HYJ42663.1"
                     /translation="MRAQCSYDYAVIRVVPRVERQEFVNAGVILWCQEQDVLEARVEL
                     DEARLGMLDASVDLDTVRRHLASISIICAGGAEAGPIGELPKRERFDWLVAPRSTIIQ
                     TSAVHSGRCNDVPSTLDRLLDAMVRPAK"
     gene            complement(14846..15628)
                     /locus_tag="VEW08_17870"
     CDS             complement(14846..15628)
                     /locus_tag="VEW08_17870"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HYJ42664.1"
                     /translation="MSLRTVNATRYVTPLREGGSLPAIVEADDAGMYVLKFRGAGQGA
                     KVLVAEIIVGEIARAAGLSMPEIVFIELDPDLARTEPDAEIQQLIRASAGLNLAFDYL
                     PGSVTFDPVADQPDALLASSIVWLDAFTSNVDRTPRNANMLMWHRKLWLIDHGAALYF
                     HHGWGDGADRSASPFPAIKDHILLKFASALDAADAAMMARITPALIQDVVALVPDDWL
                     ASDKAFASPELARESYIQHLSRRLQSPREFLEEAKRARAMLV"
     gene            complement(15636..15977)
                     /locus_tag="VEW08_17875"
     CDS             complement(15636..15977)
                     /locus_tag="VEW08_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42665.1"
                     /translation="MYEQKSDPPLIRKFFARRLCIHVLIAFGLIAFSIALGMAGYSGL
                     EHLSPLDSFLNTAMLLGGMGPVNPPVTDSGKLFAGLFALYAGLVFIVTAALLFTPLLH
                     RLMHHFHWDDK"
     gene            complement(16019..>16785)
                     /locus_tag="VEW08_17880"
     CDS             complement(16019..>16785)
                     /locus_tag="VEW08_17880"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HYJ42666.1"
                     /translation="RRHRRHRSRMRVTRIFVEQKLVSGKGVGLPEQAGLHLTRVLRLE
                     AGAPVTLFDGTGGEYEGTLERDGKKIWARVGAHTAFECESPLRVTLIQGVARGERMDL
                     IVQKATELGVTRIVPVFAERSVVKVDASKGARKLEHWRSIAISACEQCGRNRVPDVAE
                     PAPLGDAVRALEPGSARYLLVAGARETLAAAVRRDAGKPLVLMIGPEGGLADAERDFA
                     GANGFTACAMGPRILRTETAGLAALAIMQTVAGDFA"
BASE COUNT         3008 a         5540 c         5313 g         2924 t
ORIGIN      
        1 ccgagccgct tggccgcaag ttcgaacgac acgcgcgtgc gcgtcgacgg ctcggtgaac
       61 atgttcgcca cggtgatgcc ggcaagcgtg cgcttgtgcg ccggcggctc acctgcaggt
      121 ttcacgaatt tctgcgcccg cgtgagcagc gcttccatct gctgcctcga catgccctcg
      181 agcgtgagca ggtgccgcag ggtgccgtta gggcgattct gttgggtcat gtttcttgag
      241 ttgcgaacca gcgttccaga attattgcgg ccgctgccgc atccacggct tcgcgtgtca
      301 cgcgccgctt gcgctcaccg ctttcgcgtt gtgcgcgcaa gcgctcctcg gcgtcctgcg
      361 atgaccatcg ttcatccacc aggtcgaccg gcagcccgct gcgcgtagcg acttcggaag
      421 cgaaacggcg cgcggcctcg gtaagggggc ttgcggagcc gtcggcatta tagggttcgc
      481 ccacggcgat gcgggccggg cgcgtgtccg caagcgcgcg gtcgagcgcg cgccagtcag
      541 gcccgtgagg tccgttggtg atcgtggagc gggggtgcgc ggcccgagtc agcgtgtcgc
      601 cgcttgcgat gccaatacgt ctggtgccga agtcgagtgc aacgattatc tttgcgtcgt
      661 tgggaaccag gtcaggcatg accggagaac gaggtgagct gcccgggttc gacgccgagc
      721 aaccggagcg tcgcgagcca gcgctcctcg taaggcagat cgaagatcac gctttcatcg
      781 gcgggcacgg acaaccagga gttgtcgagc atctcttttt cgagctggcc ggcttcccaa
      841 ccggcgtacc cgagcgcgat gaaagctttc tcgggtcctg tccccgttgc catggcggcg
      901 agcacgtcgc gcgaagtggt gacctggatg gactcggaaa tcttgtgcgt cgagtcccat
      961 tcgccgcccg gccggtgaag cacgaagcca cggtcctggt gaacgggacc cccgcgcagc
     1021 accgggaggt cggcggcgcg cttgtccgtg ggttcgatct tcatctgctc gaatacttcg
     1081 ctgagcgtca tggtcagcgg acgattgagc actatgccca gggcgccttt ctcaccgtgc
     1141 tcgcagatca gcgagacggt ctgggagaaa ttcggatcgg caagctgcgg catcgcgatc
     1201 agcaggtgat tcgacagact ggtgacgccg ctcatgcggc gcactttacc gtgaattgcc
     1261 gggcgcggtc accgtgccgt ggcgcccggg gttctggaac tcccagccgt atttgaaacg
     1321 caacacccgg tgtttctcgg ccagttcccg ggggaatgga tcgaacgggc tcgcgagttt
     1381 caggatgtcc accaccgcct ggtcgagctt ggccgagccg ctcgaacgca cgatggtggc
     1441 cgattcgagc gtgccgtcgg cgaggatgga cacctcgacg tccgggttgc gcgttcccgg
     1501 tgcacgccag gcggcttgcg ggaaattcat ggtgcccagc cgctcgatct tgagacgcca
     1561 ggcgacgagg tatggcgcca ccagtgcttc gcgcgtgtca ggcgtcaccc acaattcgtc
     1621 gcgctgcggg ccgcgcacct gttgctcttc gtcatcatcg attccgagct tgtcctgttc
     1681 gctgttgccg tcggtctgga ccgggacctc ggtcatctcg gtcatgctgg acggcggcgc
     1741 gaagtagaga atctcgggca tgggggccgt ggtagttagg gcgcggtcgt cggcggtggc
     1801 gagcagcgcg ccggcatcat cggcgagcac ggtgccgtca ttgcggccat tgcgcgcggc
     1861 atgctcggcg gcggcgcgcg gggcacgcgc gacctcgccc gggttcacgg tgttgcctga
     1921 tccgagctgc gtgcgttgcg cgaggtaggt ggcgttctcg ttgtggcgcg cttcgggcac
     1981 gtcgtcggaa acgatgagca cctcgagacc gggagcgccg gacttctcgt gtcgcactcc
     2041 gccgaaggtg acgccgacga tgatgacacc gtgcaccagc gccgccatga agaccgtggt
     2101 ggtcaatcga tctcgcgtag gcgcgatacc ttcgcgcagc tgcatcgcgc gggccatctc
     2161 agcctttctt ggcgcctgcg cggcgctcga ttgcggcaat taccatatcg ccaatattga
     2221 gcccgaattg cttgtcgagt tcacgtattc ctgtcggact cgtgacgttg atctcggtaa
     2281 cgaatccgcc gatgacgtcg atcccgacga agaccattcc cagctccgcg acccgggggc
     2341 cgatttcggc ggctaaccag cgatcacgct cattgagctc gcgtcctaca ccggtggcgc
     2401 cagcggcgag attaccgcgg ttgtcggacg gcaaggggat acgtgcaagt gcgtaaggga
     2461 ctggctggcc gtcgatgagc aggatgcgtg agtccccggt agtggagatg tccggcacat
     2521 agcgctgcgc gatcgcgaag cgctgcccgt agtcggtcaa tgtttcgatg atgaccggca
     2581 ggttcttgtc gcctttctcg gtgacgaaaa tggacttgcc gcccatgcca tcgagaggtt
     2641 tgacgacgat cttgccgtgc tcggccgcga aggcggtgat gtcggccgcg ctgcgcgtga
     2701 tcagcgtagg cgcacagcac tggggaaacc acgcggtgaa cactttctcg ttcatgtcgc
     2761 gcagcccctg cggccggttg gcgacgaacg cgccctggat ctccgcgcgc tcgagaatgt
     2821 aggtggtgta gatgtattcg gcgtcgaaag gcggatcctt gcgcatgaac acgcagtcga
     2881 actcgccgag gcggcgcacc gtgggttcgc cgagctcaaa ccacttcgcg ggatccgcga
     2941 agacccgcaa atcccggcca cgccccaaag cgacgccatc gcgcagaaac aggtccgact
     3001 gctcgaaata cgtgagttgc cagccacgag aagccgccgc aagcaacatc gcaagtgtgg
     3061 agtccttcgc aaatttgatc ttcgcgatcg gatccatgac gacggcaaga ctagtggttt
     3121 tcggcatcgc gaactccgac ctcaaaaacg tgacgtgacc ggcatagccg ggggtattcc
     3181 tatatgttaa aacgcccctc agcttcgctt acggcgcagc attttaatag cagcccttca
     3241 atggtttaag ggcggccact ttgtttgttc aggagaggta cgcggtgaca gacaccaacc
     3301 cacacctcaa gggtctgaaa gtcctcgtta tcgatgacag caagaccatt cgaaagacgg
     3361 cggaaacgtt gctcgcgaaa gaaggctgcg aagtcttcac ggcggtcgac ggcttcgacg
     3421 cgctggcgaa ggtggccgac tacacgcccg atatcgtctt cgtggacatc atgatgccgc
     3481 ggctggacgg ttatcagacc tgttcgctca tcaagcacaa caaagttttc aaatcgattc
     3541 cggtgatcat gctgtcttcg aaagacgggc tgttcgatcg cgcgcgtggg cgcatcgtgg
     3601 gatcggagta ctacctcacg aagcctttca cgaaggacga actgctctcc gccatcgaga
     3661 cgcacgtcgc tgcgaggtga gtatggctct cattttgatc gtggatgatt ccccgaccga
     3721 agtgcacgtc atgaagaagg cgttggagaa gtctggttac cagacggcca ccgcccagga
     3781 cggcgctgaa ggcgtgcgca tggcgcgcga gatgaccccc gatctcatct tcatggatgt
     3841 cgtcatgccg ggagtgaacg gctaccaggc gacgcgaacg ctggccaacg atccgaagac
     3901 caggacgatt cccatcatca tggtcacgtc gaaaggtcag gagaccgaca agatctgggg
     3961 gctgcgccag ggcgcggtcg actacatggt caagccggtt tcgcccgatc agctcgtggc
     4021 gaaggcgcag gccacactcg cggcctgagg atcagaaaat actcttcctg aaatgaacga
     4081 gcctgcaacc ctcaagtcac tgcgtgatcg tccgtttgaa ctcctcgcgg agctcgaacg
     4141 gcgcggccgt gccgttaccg ccgccgccgc atcggagaac ccgggcgcgc gcgagtgggt
     4201 cggtgtggca tttcgcatgg ccggcgagct gtacctggcc gcccgcgaag aaacgcgtga
     4261 agtgctggcc gtgccgcccg ggctcacgcg cgtgccgggc gccaagccct gggtcaaggg
     4321 catcgccaac gtgcgtggcc agcttctgcc catcctcgac cttcgccagt acctgggttc
     4381 gggcctcacc ccgaactcgc gcaacgtacg cgtaattgtt gtcaatcacc gggaagtgcc
     4441 ggcaggcctg gtggtcgatg aagtgctcgg ctttcgccga ttctccgaat ccgaattttc
     4501 aggcgacgct ccccccaccg tggtgcgttg cgaacgatat ctcgcgggtg cgttccggcg
     4561 cggacccgaa caatggccgg tgttgagcct gcggcagctg gtggaaagcc cgtccttcca
     4621 ggaggccgcg gcatgagcac cgaagtcggc ggcggctcgc gcacgctgaa atccatcaac
     4681 acactcatca ccacgacgct ggtgtgcgtg gtgttgggtg ccctggcctg ggttgcagcg
     4741 tacctgctga gtctcaccat tctttatgcg gtggcgggcg tgctcgcgat cgtggccgcc
     4801 gtgccgctgt tcctgatgtt cgggcgtgtg cgcgcggtcg aagcggacat tggccatcgc
     4861 gaagcgctgt cgctcgcgca gcgcaaggaa actgaacgca accagcaggc cattcttcgg
     4921 ctgctcgatg aactcgcacc gctcgcggac ggcgacctca ccgtccaggc gacggtgacc
     4981 gaagacatca ccggcacgat cgccgattcc atcaactacg ccatcggcgt gttgcgcgac
     5041 ctggtgagca ccatcaatca gtccgccatc cagctcgacg gcgccacccg gcagacgcag
     5101 gcgctgtcag cgcacctggc gaaggcgtcg agcgcgcagt ccaagcagat cgccggcgcc
     5161 accgaatccg ccagcaacat ggcgagcagc actgaagcca tctcgggaaa ttccgagcgc
     5221 gcttcggacg tcgcgcgcca ttcggtggac gtggcccaca agggcggtga cgccgtgcgc
     5281 cgcacgatcg acggcatgaa cgcgattcgc gaaacgattc aggagaccag caagcgcatc
     5341 aagcgcctgg gcgagagctc gcaggaaatc ggcaacatcg tcgagctcat caacgacatc
     5401 gccgagcaga cgaacatcct cgcgttgaac gcctccatcc aggcctcgat ggccggtgac
     5461 gcgggccggg gcttcgcggt ggtcgccgac gaagtgcagc gcctcgccga gcgcgccgcc
     5521 gccgcgacgc gccagatcga aacgctggtg cgcgccattc aggcggatac caatgaggcc
     5581 gtggtctcca tggaacgcag caccaccgac gtggtgggcg gcgccttgct ggccgagaac
     5641 gccggcgcgg cgctcgagga gatcgagcag gtctcgaacc agatcgcgag tctcgtgaac
     5701 aacatcttca gcagctcacg ccagcaggtg tccgaagcgc agaagatcgc gcggaacatg
     5761 caggtgctca aagagatctc cacgcagacc gccgagtcca ccacggcgac ctcgcaaagc
     5821 atttccaagc tcgcgcaact gtctgccgcc atgcgtcagt cggcgtccgg cttccgtctg
     5881 cccggcgcgc ccgtgccgcg ccaggtggcc gcggccggag cggccattgc cgctgccgcg
     5941 gcagcaacca ccggtatcag cccggcccag ccctcgaccg gcgccaccac gcgcacgcga
     6001 gccatatcgc ttgccgcctc gtaacacgga cgtatagagc tgccctcgat gcctgaagtt
     6061 gccttacaaa ctctcgatct cgtcggccgc gagctgaaca acacgctcgg cgaggcgcgc
     6121 accgcgctcg agacctttgt cgaacagccc gaaaacgtgg tgctcctgca gcgctgcgtg
     6181 tccgacctgc accaggtcca gggcgtactg cgactgctcg aaattttcgg cgccgcgttg
     6241 ctggccgagg agatggagca ggtcacgctc tacctgcaga cgcccgcttc gcaaaagaac
     6301 caggccgaaa ccctggatgc gctcatgcgc gccatggtgc agttgccgag ctacgtcgaa
     6361 cgcgtgctgg ccggcggacg tgatctagcc ctggtgctgc tgcctctgct caacgacctg
     6421 cgcgcggtgc gcggtagccc gttgttgtcc gaaggcactt tgctgctgct caacctcaag
     6481 tccgatcaac aggcggcgcc cgtcaccccg aatgcgaatg agcccgcgct cacggtggcg
     6541 cagtgggctc gtcggctgcg cacacgattt cagctgggcc tcatcggctg gatccgcggc
     6601 gagcgccccg agcagaacct cgagatcctt gaaaccgtcg cacaccagat cgagcaggtg
     6661 gcgacgcggc agtccgtatt ccagctctgg tgggtggtgg gcgccattgt cgaggcactg
     6721 cgcgaaggcg gcctcgaaac cggcgtctcg gtcaaacgcc tcctcggcct ggccgatcgc
     6781 gaaatcctgc agctctatac gcagggcgaa ggtcgctatt cgcagaaccc gccggtcgag
     6841 ctgctcaaca accttcttta ttacgccgcc cgcgccacca gcaatggccc gaaggtcacg
     6901 gcggtgcgcg cctcgttccg gctcaatgaa ttgctgaacg tcgacgactc ggtcgagcag
     6961 gagcgagaga atctttccgc gccctcggtg aagttgatgc gcacggtcgc cgcggccatc
     7021 cgcgaggatc tgggcaaggt caaagacgta ctcgatattt ttgttcgtcg cggggggcag
     7081 cccgccgagc tcgagccgca ggtcggcatg ctgcgcaaga tcgcggacac gctgggcgtg
     7141 ctgggtctcg gcgaactgcg ccagctcgtc atcgaagaaa cgaaccgtct cgaagccatg
     7201 gccgcgggca agaccgcctc ggatcatgcg gcgctggtgc acattgccgc cacgctgatc
     7261 aacgtcgaag accgcctcga ccgcgacctc atcggactca tcgttcccaa gacgcaggtg
     7321 cagcaggaag aagcgggctc cgtcgacgcc gacttccagc aggtgcaggc cgcggtgctg
     7381 cgcgagtgca gcgtgaacct ggtgcgcgtg aaagaagcca tcgcctcgaa tgtcgccggc
     7441 acgctggacg tgggtgcgct cgacgcctgg cccggcctcg tcgccggaat caaggccggc
     7501 ctgctcatgc tgggcaaatc ccgcgcggcc gagatcgtcg atgggatcgc cagaaatctc
     7561 aaggaattgc tgcagcccgg tggcacggcc gttccaccta actacctcga ccggctggcg
     7621 gacgcggtgg tcagcctcga gtactacatg gagacgctgc aggctggccg cacggatccc
     7681 tggtacatgc tcgacaacgc gcagacctgc ctccaggcgc tgtcgcagat gccgaaacgt
     7741 gtggtgccga cggtgccgcc gctggggccc gaagcctatg cggccacggt gctgctggcc
     7801 gatgccggtg ctaccgcgcg tgcgcatgcg ctcgccgccg gcaacccgcc caccatccag
     7861 ggtgtcaccc cgacgctgca cacctccggt cagccgccca tcggcgccaa gcccatcaac
     7921 atcgaccccg agctgctcac tctttatata gaggaagccc gcgaagaggt cgcacgcatc
     7981 gccaagctgt tccccgcctg ggaacagaac ccgctggaaa cggatgcgct cgccggcgtg
     8041 cgccgcgcgt tccacacgct caagggttcc ggccgcatgg tcggcgcgac ggacatcagc
     8101 gaattcgcct ggtccatcga aaacctgctc aacaagatca tcgagaacac gttgcagcgc
     8161 tccccatcca tcctcgccgt catacgcgac gcgagtgccg tggcggggga actggtgact
     8221 gcgctcgaag cagggcaagg cgcgcccgcg aaggtgcaga gcatcatcga tcgcgcgcat
     8281 gcgctggccg ccaacaggcc cagtgccagt tcgcagactg cgaccatgga aattctcgaa
     8341 cgcacgctcg aaacgcgccg cgatgacgtc gtctccgcga ccaaccgtgt gccgacgctg
     8401 cagccgcccg ccgagccggc acccgcggtc agcgagagct cgatcggcga gagcacgatg
     8461 gaagtcgtcg aagactggtc cgccgccgcg caggaaagcg gcgagaacat cgtgctgtcc
     8521 gcgcccgaag aagagcccag tggcgacatg cagcttcgcg acatctacag ccgcgaaaca
     8581 caggtgaaca tcgccgccgt cacgcagttc atcgaaggcg agcgctcgcg caatgcgccg
     8641 cacgtcgtca gcgaggaagc ctatcgcgcc tgccacacac tttcgggcag ctcacgcatg
     8701 gccgaagcgc gccacggcat ccgcctcacg gatccgctcg agcactgggt gcgcaagtct
     8761 ttcgacagcg gcgtgggcct cgaagccgat gagctgaatc tgctcgccga ctgcatgaat
     8821 gccatgcaaa cggttgcctc gcatctagac gagtccaccg gtttcttcca gtcacacagc
     8881 acgctgctga cgcgcatcga tcaggccacc gaaaatctcg aacagcgcat cgtcgccgca
     8941 gcccgcgccg ccgagctcgc caatgcgcca gctcccgcgc cgcccgcggc gtccccggtt
     9001 tccgtcgcgc cggcggtcgc ggcgcctccc gcgtcctttc cgccggtagc tgccactccg
     9061 ccagtagcgc ccactcctcc agcagctctg gtcgaagacg tcctcacgga tttcgaccct
     9121 gacatcgcca gtatcttcac cgatgaagcc gtcgagctca tcgaacaggc gcagagcgcg
     9181 ctcgcgcaat ggaacgagaa ccgcagcagt gtcgatggtc tcgatgcgct gaagcgtccg
     9241 ctgcacacac tcaagggcgg cgcccggatg gcgggcctca tacccatggg cgatctgagc
     9301 catgaactgg aaaccctgtt catgcagatc gacagcaaag tcgtcgcgcc cgacgaccgc
     9361 gcgttcggcc tcgcgcagac atcgctcgac gaactcgcgc gcatgcgcga atcggtgagt
     9421 tcgggcaagg gtgttccgac tgccaacgcg ctcatcgcaa aaattcacgc gctggtcagc
     9481 gggactccgg ccgccaaaga tcccgcgcca ccgccgccag caccgccggt tgcgcctgca
     9541 gccatggcgc caccgcctcc acccgcgcca gcgccccccg tggctgcgcc ggcgccagtg
     9601 atggctgtgc cgccgccgcc gcccatcgtc gcgccagcgc ccgcacctgc gccggtgccg
     9661 aggccagcgc cggttccgac gatggttcct acgatttcgg ccgtgcctgc gcccaaagcc
     9721 tcgtacgtgc cgccacctcc gcgcgccgaa cccgcgcccg agcccccggt gcttgcggcc
     9781 gtgtgggcac cttcgtccga gccgccgtcc atcgtgacgc gtgccgcggc gagggaaccc
     9841 atgtcggcgg ggttcctccg tgatggcgcg aacaaggacg ccgaaggcgc cggcgatggg
     9901 ccgctgctgc ctccgggcgc agtgccgcca ggccgcgaac cgaccgcacc ttccgatcgt
     9961 cccgagctcg cgcgcgtcga cgccgaattg ctcgaccagc tgctcaacaa ctccggcgag
    10021 gcgagcatcg cgcgcgcccg tctcgaacag cagatcggtt ccatcgactt caacgtcggt
    10081 gagttgtcac gaacggtcac gcgcctcaag gagcagctgc gcaagctcga gctcgaaacc
    10141 gaggcgcaga tcctgcaccg cttcgaagag gaaaagggcc agaagagcga tttcgatccg
    10201 ctcgagctcg accgctactc ctcgatccag cagttctcgc gtggtctcgc tgaaacggcg
    10261 aacgacgtcg gcagtattca gcagctgctc gaaactctca cgaaggacac gcaaaacctg
    10321 ctgacccagc aggcgcgcac catcaccgaa ttgcagaacg gcctcatgcg cacgcgcatg
    10381 gttccgttcc agcgtcacgt tcagcggctc gcgcgcatcg tgcgccaggc cgccacggac
    10441 acgcacaaga aggccgaact gcagatcgaa ggcgcctccg gcgagctcga ccgccaggtg
    10501 ctcgagcgca tgatgccgcc gttcgagcac atgctgcgga acgcggttgc gcacggcatc
    10561 gagaaaccgg aggaccgcaa gaaggccggc aagagcgaga gcggcaccat caccatcgcg
    10621 ctgcatcgcg aaggatcgga agtggtcgtc gaggtttccg acgacggcgc cggcatgaat
    10681 ctcaaggcga tccgcgacaa aggcatttca gtgggcctgg tgcgcgccga ccagcagctc
    10741 tccgacgaag acatcatcca gctcgtgctc gagcccggct tctccaccgc gggcgcggtg
    10801 agcacgctct cgggtcgcgg cgtcggcatg gacgtggtcg cgagcgaaat caagaagctc
    10861 ggtggcgcgc tgcacatgga gacgaagcag ggcgaaggct cgcgcttcac catccgcctg
    10921 ccgctgacgc tggccatcag ccacgcgttg atcctgcgcg ccaacgatga gctctacgcg
    10981 ctgcctcttc ccacggtcga aggcgtcgtc cgcctgtcgc gcaccgaagt cgaggcgcat
    11041 ctcggccccg aagcgccggc gttcgaatac ggcggccaga aatacaagtt ccagcacctc
    11101 gcgttctacg tgggacgcga acccggtccg ctgcccgatg gcgacgtaac cgtgcccgtg
    11161 atcctcgtgc gcgccggcga acattccacc ggtctcgtca ccgatgagct cgtgggcagc
    11221 cgcgaaatcg tcgtgaagtc ggttggcccg cagatcgcgg ccattcgtgg aatatcgggc
    11281 gcaacgattc tcggcgatgg ccgtatcgtc atcattctcg acatcggcgc gctggtgcgc
    11341 accgatttcc gcgcgcgcac cgaaccggtg atgccacgcg agaagaccga cacgcgcagc
    11401 gtcgcgctcg tggtcgacga ttcgatcacc gtgcgccgcg tgacgcaaag gctccttgaa
    11461 cgcaacggca tgcgcgtgtt gaccgcgcgc gacggcgtcg atgccatgga aatgctccag
    11521 gagcacacgc ccgacatcat cctgctcgac atcgaaatgc cgcgcatgga cggatatgaa
    11581 gtggcgacgc aggtgcgcgc ggatccacgc ctctccggca tccccatcat catgatcacc
    11641 tcgcgcgtgg gcgagaaaca ccgcgcgcgc gccatcgaga tcggtgtcga cgactatctc
    11701 ggcaagccgt accaggaagc gcagctgctc gaagccatcg agccgctgat cgcgcgccgc
    11761 cgcgaacaac atatgcgtga ggcgaactag ttcgaaggat ggggacatgg cagagcgcat
    11821 aacagaaatc tacagcctgc tgatcccgct gctcgacggg cggttgatca tcccgcgttc
    11881 ctgcgtcgcc gaggtcgtgg gcttccagcc gccgtcggag atgccgggcg cgccgccgtg
    11941 gtatctcggc acgttcgcct ggggcagccg tcaaattccc ctggtgtcat ttgaaggcac
    12001 ttgcggacaa accattccgc cgctcacggg ccggacgcgc gtcgtcgtgt tgttcgcgct
    12061 cggcaaccag gtggaaggcg gctactacgg catagtgtcg caaggctttc cacagctcgt
    12121 tcgcgtgagc gccgacatcc tgcggcccga tccgaccaag acctttcccg agcgcacgcc
    12181 catcatttcg caggtgcgaa tgatcaacga agcgccgtgg atccccgatc tcgagcgcat
    12241 cgaagagatg atttcggaag agacctccgt cctcgtcgag tgatgctgcg caagagttgg
    12301 ctgtcagaat cggccgatgt gatttgaagg catccacgga caagctgtcc aaccactaac
    12361 taccagtttt gtcgcgccga agcgacgatc gcctaagtgt tgtccacgat cgtcgaatag
    12421 actcctgcca tggcagaaac catggctcat cactatcgcc ctgatgtcga cggcctgagg
    12481 gcgctggccg tcgtggccgt cgtggcattc cacgcggcgc ccaagctact ccccgccgga
    12541 ttcctcggcg tcgacgtgtt ttttgtcatc tcgggcttcc tgatatcggg cctggtgctg
    12601 gatcagcttc gcgccggcaa attcaccatg tcgggcttct acctgcggcg cgcacgtcgc
    12661 atcctgccgg cgttgctgct gatgctggtc atctcgagcc tgatggcgct ggccatcctg
    12721 atgccggacg aaatgcagga cttcgcgaag ctgctcatcg ccagtgtcag cttcgttccg
    12781 aacctcgcgc tgctcagtga gaccggctat ttcgatgtcg gcgcccacat gaagcccttg
    12841 ttgcacatgt ggtccctggg tgtcgaagag cagttctatt tcatctggcc gtttttcctg
    12901 atgctcatgg cgcggcgtga tcggccgggg cagacgctgc tcatgatcgg cgtgctggcc
    12961 gtgctgtctt tcctggttca cctgttcatc ttccgcgact ccccggagtc cagcttctat
    13021 ctacccttca cgcgcttctg ggagttgatg attggcgcgg cgctggcggc aatcccggcg
    13081 ggcgcccggc catttggcga gtcacgaccg ctccacaacg ccatttccat cgtgggtctg
    13141 ctcctgatgc tcggcgccat gatgtggagg ggcggggatg agctgagccc attcgtgggt
    13201 gtggctccca cgttgggggc ggccatgttc atcgccgccg gacctctcgc attgccgaat
    13261 cgatcagcat tctcgtggcg acccgtggtc tacgtcggtc tcatcagcta tccactctac
    13321 ctgtggcact ggccgctgct gagcttgctc cgcatcgcgg acgtcgaaga tcacgccacg
    13381 cagcgcatgc tgaagatcct catggtggtg ctcgccgtgg tcctcgccat cctcacgtat
    13441 cacctggtcg aacggccctt gcgccggcga aacgatctca ggcgaatcgg tgcgtacctc
    13501 gggttgcttc tggtggccgt aggcgcctgg ggtgtcgtga tcatcgtgag tgatggtttt
    13561 ccgaaccgca cgacgctccg ggagaacccg atcccttggc cagccgaact gcgcaattcg
    13621 ccgcgttgca tggaagccga gggatttgcg cccgatcttc gcgaggaagc cctatgcacc
    13681 cgtagtgact acgaccgcac accacggatc gtcatcatgg gagattcgca tgcaaatgcg
    13741 atctcgcccg gcgttttcgc cgcatatccg gaggattcgg tgctggccat cggcgcttcc
    13801 gcgtgcctgt acctgcggcg cacggatttc tggcatgcag cggctccagg caagcgcggt
    13861 acttgtcccg ggttgatcga caccgcctat ctcacgatca caccggatac ccgcctggtc
    13921 atcctggccg cgcgctatac gatgtacgtc gggtctgccg ccgaagccgc cgagatgttc
    13981 gacttcccgg gcacgcaata tttcagctcg ccggatttcc ccggggccgg aaagatcgaa
    14041 gcgttcgagc gctcgctgag ccgcgacctc agcctgttgc tcgagtcgga tcgcgaagtc
    14101 gtgctggtgt tgcaggttcc ggaactggac ttcctgccca aacgttgttt gcatgcccgc
    14161 ccgttccagc gcctgctgcc caagcccgag gtgcggtgca gcgtgccacg agcgagggcc
    14221 gagcggcgtc aggcgagcta tcgcgcggca gtttcacgcg tcgcgagagc gttgaacaac
    14281 cctcgacttc atgtcgtcga tcccatggac gtgttgtgcg acgccaagga atgtcacgcg
    14341 atgatcggcg gcatgttgat gtatcgcgac gatgatcacc tgagcatcgg gggatcgaaa
    14401 tacgtctggg agaagatcag gcctcgcgac ctccgggcat tcgcgccgcc ggctgcgcgg
    14461 tgaggcgcgg tcccgtcact ttgcgggacg caccatcgca tcgagcagcc gatccagcgt
    14521 cgacggtaca tcgttgcaac gaccgctatg caccgcagac gtctggatga tggtgctgcg
    14581 cggtgcgacc agccagtcga agcgctcgcg ctttggcagc tccccgatcg gacctgcctc
    14641 ggcgccgccc gcgcagatta tcgagatgct cgcgagatgc cgccgcacgg tgtcgaggtc
    14701 cacggacgcg tcgagcatgc ccaggcgggc ctcgtccagc tcgacacgcg cttcgagcac
    14761 gtcctgctcc tggcaccaca ggatcacgcc ggcgttgacg aactcctgac gctcgacgcg
    14821 cggcacgacg cggatcaccg cgtagtcata cgagcattgc gcgcgcacgt ttcgcctcct
    14881 cgagaaattc acgcggcgac tgcagccgcc gcgacaaatg ctgaatgtac gattctcggg
    14941 cgagctccgg cgaggcgaac gccttgtcgc tcgccagcca gtcatcggga acgagagcga
    15001 ccacatcctg gatgagcgcc ggcgtgatgc gggccatcat ggcggcatcc gcggcatcga
    15061 gcgcgctcgc gaacttcaac aggatgtgat ccttgatggc cgggaacggg ctcgccgaac
    15121 gatcggcgcc atctccccag ccatggtgga aatatagcgc ggcaccgtga tcgatcaacc
    15181 aaagcttgcg gtgccacatg agcatgttgg cgttgcgcgg tgtgcggtcg acgttgctcg
    15241 tgaacgcgtc gagccagacg atggacgagg cgagcagcgc atcgggctga tcagccacgg
    15301 ggtcgaaggt gaccgagcct ggcagatagt cgaacgcgag gttgagcccg gcgctggcgc
    15361 ggatgagctg ttgaatttcg gcatccggtt ccgtccgcgc gagatccgga tcgagttcga
    15421 tgaacacgat ctccggcatg gagagtcccg cggcgcgcgc gatttcaccg acgatgattt
    15481 ccgccaccaa cacctttgcc ccctggccgg caccgcgaaa cttcagtacg tacatcccgg
    15541 catcatcggc ttcgacgatg gccggcaacg agccgccctc acgcagcggc gttacgtagc
    15601 gggtggcatt gacggtgcga aggctcaccg gttcttcact tgtcgtccca gtgaaagtga
    15661 tgcatcagcc gatggagcaa cggcgtgaac agcaacgccg cggtgacgat gaacaccagg
    15721 ccggcgtaca gggcaaacaa ccccgcgaac agtttgccac tgtccgtgac cggagggttc
    15781 accgggccca ttccgcccag caacatcgcg gtgttgagaa aggagtcgag cggtgacagg
    15841 tgctcgagcc cgctgtaacc cgccatcccg agcgcaatgg agaacgcgat caacccgaaa
    15901 gcgatgagca cgtgtatgca caggcggcgc gcgaagaact tccgaatcag gggtggatcc
    15961 gatttctgtt cgtacatgcg gtacctcact atcttcccgg aatgtagctc gacgcgcgtc
    16021 acgcaaaatc gcccgcgaca gtctgcatga tcgcgagcgc cgcgagaccg gcggtttccg
    16081 tgcgcaggat gcgcggaccc atcgcgcagg cggtgaagcc attcgcgccc gcgaagtcgc
    16141 gctccgcgtc cgcgagtccg ccctcgggcc cgatcatcag cacgaggggt ttgccggcat
    16201 cccgccgcac cgccgcggcc agcgtttcgc gagcgcccgc gacgagcaga taccgcgcgg
    16261 agccgggctc gagcgcccgc acggcgtcac ccaatggtgc cggctcagcc acgtcaggta
    16321 cgcgattgcg cccacattgc tcgcaggctg agattgcgat cgaccgccag tgttcgagct
    16381 tgcgcgcgcc cttgctcgca tccaccttca ccacggagcg ctccgcgaac accggtacga
    16441 tgcgcgtcac gccgagctcg gtggccttct gcacgatgag gtccatgcgc tcgccgcgcg
    16501 ccactccctg gatcagagtc actcgcagcg gcgattcgca ctcgaaggcg gtatgtgcac
    16561 ccactctcgc ccagatcttc ttgccgtcgc gctcgagcgt gccttcgtac tcgccaccgg
    16621 tgccgtcgaa gagcgtgacg ggagcgccgg cctcgaggcg cagcactcgc gtgaggtgca
    16681 ggccggcctg ctcggggagg ccgacgccct tgccggagac cagcttctgc tcgacgaaga
    16741 tacgggtcac gcgcatgcga gatcgatgcc ttcggtggcg acgcg
//
LOCUS       DASQJS010000452        16033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3445510, whole genome shotgun sequence.
ACCESSION   DASQJS010000452 DASQJS010000000
VERSION     DASQJS010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16033
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3445510"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VEW08_17885"
     CDS             <1..379
                     /locus_tag="VEW08_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42667.1"
                     /translation="GREMSSVPFGGGRIGSRIDEAVKLEPKNPRVRLVEALAMFDRAG
                     KNADEQAVATRNLRAVTVMFEQARAAASSTPEWGAAEAYAFLGRALFDQRDVVGAREA
                     LERSLLIAPDYAFARKLMAQITR"
     gene            475..2475
                     /locus_tag="VEW08_17890"
     CDS             475..2475
                     /locus_tag="VEW08_17890"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HYJ42668.1"
                     /translation="MNYPRLFAPLDLGFTRLPNRIVMGSMHTGLEDRARDFPKLAAYF
                     AARARGGAGLLVSGGIAPNRAGWAKPFAGKLSAPREVARHRLVTDAVHAEGGKICMQI
                     LHTGRYAYHPLPVAPSALRAPINRFKPRALSSDGVRDQIADFVRCAELAQQAHYDGVE
                     IMGSEGYFINEFLAPRTNLRTDEWGGSLENRARLALEVVRQTRVAVGPNFIIIFRISG
                     LDLVEGGGTGAEVEWLASQMEAAGATMLNTGIGWHEARIPTIAGTVPRGAFGWVSARI
                     KAATRLPVIATNRINAPEGAEALLANGDADLVSMARPLLADPELPRKAAEGRDSEINT
                     CIACNQGCLDKVFENQRATCLVNPLAAYETELIIASTRAIKRIAVVGAGPAGLACATT
                     LAERGHRVTLFERASEIGGQFRYAREVPGKEDFHDTLRYFARRIELTGVDLKLNTTAD
                     ASRLASEGFDAYIISSGVAARVPDIAGIDHPKVISYPDLLSGARRAGDSVAIVGAGGI
                     GFDVATFLTHEADSNYFEEWGIDRTLTQRGGLVPPRPVASRRRVYLLQRKPGRLGATL
                     GKTTGWIHRSALRHRGVVMRNGVSYQRIDDAGLHISTEAGPELLAVEHVVICAGQESV
                     NGLATELAATARPVHVIGGALLAAELDAERAIREGVMLAAKI"
     gene            2472..3266
                     /locus_tag="VEW08_17895"
     CDS             2472..3266
                     /locus_tag="VEW08_17895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HYJ42669.1"
                     /translation="MSGFDGNKNGDSPQFEMAFPTANDGGRIGDSPLIRLSARARRLS
                     IRVYPDARVEVVVPPRARPREVELFIATHREWIDSKRAVALRNRPEPQPFPPATIELR
                     VSAETWRLHVAGGAGRLRLAEMSGGIRERVLRVTGAPSSDKLRAALRSWLLRTAQRLL
                     APRVAALAAATGVSYSQIAIRRQRSRWGSCSARGTISLNCCLLFQRPEVVDYLIVHEL
                     MHVKHMNHSARFWQAVERHCADWRALDRELVQGWRHVPRWVFSEGS"
     gene            3274..4113
                     /locus_tag="VEW08_17900"
     CDS             3274..4113
                     /locus_tag="VEW08_17900"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HYJ42670.1"
                     /translation="MGDNKIDLKNEGVHWDLGTSQSYGEYLQLDKLLDAQKPNSIEQD
                     EMLFIIVHQVSELWMRLFLHELDHVFECVRRDNLDPSFKMLARISRLQEELLGAWSVL
                     STLTPFEYSSFRNTLGRSSGFQSLQYRLLEFKLGNKNRDVLAVHKRDPAGFERLELAL
                     NSPSIYDEVLRLLSRRGYGIPDELIERDFSEPYVASKAVTAAWLGVYHNAEKDWDLYE
                     MAERLTDLDQKFQLWRFHHLKTVERVIGYKPGTGGTGGVSYLAKALELKFFPELWHVR
                     TSM"
     gene            4110..4805
                     /locus_tag="VEW08_17905"
     CDS             4110..4805
                     /locus_tag="VEW08_17905"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYJ42671.1"
                     /translation="MSALVAGLELDHIIFFCAPAAPEAEALLARGLHEGPGNSHPGQG
                     TVNRRFFFRNVYLELLWVEDFDEAQAPDARRTGLWDRWINRHNGSCPIGLVFRPAAPD
                     TPTPFPSWSYRPRYFPSGFSIEVALDIPPNEPLLFYLPFARPALVEDVVQVPGGAQIG
                     PVDGTTLHLPETSGLSAALNSLVAAGVVSVEPGREVLVDLHHQNTSSEIIDLRPKLPL
                     RFLPAGARRHSAH"
     gene            4889..7237
                     /locus_tag="VEW08_17910"
     CDS             4889..7237
                     /locus_tag="VEW08_17910"
                     /inference="COORDINATES: protein motif:HMM:NF045042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase"
                     /protein_id="HYJ42672.1"
                     /translation="MKRGMVSAAASACLLIAYASLAEVAPPTPKYQVENLGRGLLALR
                     TGDSSVYVGWRLLGTDPAGLAFNLYRQTGDAAPVRLNESPLLATTDFVDTTADLTLGN
                     AYSVRPVLQGVELTASGAFSLPANAPTQQYLTVPLQRPAGGNVEVPPGHVTQAVTYSP
                     NDASVADLDGDGEYEIVLKWDPSNARDNASAGLAGRQLLDAYRLDGTLLWRIDLGRNI
                     RAGAHYTQFIVADLDGDGKAEVACKTADGTVDGAGTVIGDATRDYRSLLVPTDGIQVP
                     ATNDARYGKVLAGPEFFTVFNGLTGAALATTNYLPGRDPIGGWGGIGGNGNSDTNGNR
                     GDRFLAGLAYLDGKLPSVIMARGYYGRSVIAAWDYRDGQLTSRWVFDSGVPTATGFPW
                     AGSSPFSGQGNHNLSVADVDADGKQEIIYGSMVVDDNGVGLFSTGLRHGDALHAGDLV
                     PSRPGLEVYGVHESEGNTLSLGTPGMALYDARDGQIIWSLLPGRDVGRGMASDIDPRT
                     PGYEFWGATEVGLLDGQGNRVADSPSTVNHAVWWDADPLRELEDANWVSKWDWNSSTL
                     TRLLTADGATSNNGTKQNAAITGDILGDWREEVVFRSADNLSLRIYSTSIPATNRLFT
                     LMHDPQYRTAIAWQNVGYNQPPHPSFFIGDGMAEPSAPPVWHLDTDAPAFRKLSPSLR
                     SLPPLGILLPVFIKAELVDLLDDKASARIVNVTSSGDDDKRRRHELPDVVILDDLLVL
                     LRAERDGRADRVYTIDVEGVDASGNTVVESVEVTVTRNWFPW"
     gene            complement(7314..9461)
                     /locus_tag="VEW08_17915"
     CDS             complement(7314..9461)
                     /locus_tag="VEW08_17915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HYJ42673.1"
                     /translation="MSVRVIEIAARVLGTVALAGLVSCSAAPSGTSGTTLVADAGEAH
                     SYIVEADSTDNAARAVKAAGGKVVSRLGVIDAVEASLTSSQHQAVLSIQGVKQVTPNA
                     PVTTLAAAYVRDNFETGSFANNNGSHRWYGDWVEQNDNNSPYNGKVVIGWLERGGNRM
                     IIGSNGAVYRRAATPSSASSVTLKLNYSRASLEAGEYVAVQASANGGSTWTEVGRISG
                     AGNDTGFTARSYNITAYRGRNTAIRFVSSMSEGFPGDYVDLDDVEISYNTTYGEGDPT
                     PVDVNAKALHSAGIRGREIGVAVIDTGYWKIDSLDVDSNGLGRMAVQYDAVSNTVVSN
                     WAPVSTDTTGHGTHVTSLIASSRKKSTGEYFGVAPDARIISIKAFGEDGSSNYATVIR
                     AIDWVITNKNPLAIRIINLSLGAQAQSRYWDDPLNKAVMRAWQSGIVVVVSAGNSGPL
                     PQTVGVPGNVPYVITVGAMTDNFTTNKSDDRLASFSSTGPTYEGFVKPDLVAPGGHAW
                     GFMGTYMKIAVDHPTYMNNGDFFTMSGTSQAAAVVSGVAALVLHQNPSLTPDQVKCRI
                     IASAKPAVDANGKLAYSVLQQGTGMVDAERAVYGTASNCANQGLNINADLAGTQHYQG
                     SVRQKADGTFTVSTPSGTLNEQGYIWNNGYIWANGYIWANGYIWANGYIWANGYIWAN
                     NYDIPWVDGYPAQIGSTASSASSMSINFWVNQE"
     gene            complement(9619..11304)
                     /locus_tag="VEW08_17920"
     CDS             complement(9619..11304)
                     /locus_tag="VEW08_17920"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HYJ42674.1"
                     /translation="MDSDIRDALARSLPDLALIVRRDGVILSCVGGQAVRGLDAQEEA
                     LPGRTLDTLWPESVASHALQTVRRILKTRKPAQQRYQDGDLKLEMRFQVQGFDRVLVV
                     CRDLGEGVTANDSGLYPQSASAMLPHRDGFEKSLRMALDMATLREDTVGVVLIHIGGY
                     ATYQRVLDSGIANRLLECAAKTIAETLPEARTSLPRIARVSDDVIGVVLTDIRARADA
                     DVAVEAIMKALQHPVDLDGQIFQLAPQSGVTLSGTDGDRASELIERALTTLDAGRRQG
                     IDRLVTFYSDTLRLASLTRLDWSQELSQAIQKDELELHYQPRFLLSTREIVAVEAFLR
                     WPHKIRGLVPTSEFLPIAELSGLSVQLGRWVLRRACRDLAVLAERGYPALRVSVNVGR
                     QYLSAESLAEDVTNSAREAGIEPSQLDLEITEQMLSTGRTGLAALHQLRGQGVRVLID
                     DFGTGYISLGKLRSSPLDGIMIDRSFVEEVGHDHEARAVCRAAIALGHAFGLRIVAEG
                     IETEAQAQFLIAEQCHEGQGHLFCAATPLSNLLDKILGQGKEAPASVQRTAQR"
     gene            complement(11320..14568)
                     /locus_tag="VEW08_17925"
     CDS             complement(11320..14568)
                     /locus_tag="VEW08_17925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HYJ42675.1"
                     /translation="MRARVAAAAAACIFSTVALATPPLSVQRYSLEEGLSQQAVNAIV
                     QDSEGFMWFGTEDGLNRFDGYDFRQLRHDRGDSETLPNGWISSLVASEDGLWIATDGA
                     GVVFRNAVTGKLNAPPILRDAPDLQRVRTLARDRLGRLWIATRDEGLAIFDAHSNELR
                     RLRHSSALPNSLSSNSVFSLVNLRNGDTLVGTASGLDRLTTANLDVTRIALPTELVAA
                     GQPLRVRALAETPDGMVWVGTDMGLARFDPRNSRWRVYHELPEHAPGRSAALPDNRIQ
                     SLLIDSQGRLWVGTMRGLSWFDSATEIFSSYRRDEAEARSLPDDYIVSLAEDRGGSLW
                     IGTKFGGLAKWNPRTWSFGHVRASAEEGYSDHNITSFAEDKLGRLWIGTFGSGVNLLD
                     RSSGRVSTIRHVQGVHSSLSDERVMSMLEGSNGDMWVGTMGGGLNRFDARTLKAEVFL
                     HDPAVPTSLGAAGVMSLLEVDKQLWVGTFGGGISRFDARTRRFENLRPGPEDGLHLSS
                     GRVTALARDRSGHVWIGTDGGGLNVWDQKTRRLYYYKRGAKQLDTLSADTIYSILVDD
                     TGGVWIGTRGGGLDRVLNPADAPHGLRFANFSEAQGLPNNSVYGLRADGTGDIWISTN
                     FGLARLDPKSGVIQRFHRLHGLQAEEFNFGSHYRDRSGKLFFGGAAGFNTFYPEVLEF
                     NERPPRVVLTQFLKLNTPGIVGVPEERIERLNLGHKEDVITLKFAALDFADPRSNRYE
                     YKLDGFDSDWVRADERRVATYTNLPGGQYVFRVRASNSDGVWSTQDLALPIDVAPSPW
                     LSPVAYLGYAMLAVLMLLTVWYAQQRRIARAAMQRLELEQQVSDRTYELAQRNRELED
                     ANRRLEMASFSDPLTGLANRRYLMQQFPRLIADRKGNSGLAIMIIDLDALKPINDQHG
                     HAAGDDVIVEIAKTLRQAIRPDDVLARWGGDEFVVVAQAQHVEHACMLAERVRERIAK
                     MKCVLPRGAVVRTSCSIGLTCLPFVPGNPESVSWEQAIKIADLALYRAKRGRNAWRGW
                     FGTEHVSSLQSVITAVESNVDSLIANGVIIEQMSTRGSDDTVNALRVLREAR"
     gene            complement(14767..15414)
                     /locus_tag="VEW08_17930"
     CDS             complement(14767..15414)
                     /locus_tag="VEW08_17930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HYJ42676.1"
                     /translation="MPIAIFDLDGTITHRDTLFPLVLRQLAKPRPWRLLRLLKVLPAT
                     IRFAFDHDRAALKQSLLRATLRGTPRAELHAAAAAFVTETIARRSFADALAAIRRHHD
                     AGHHMVLMSASVDFYVPEFGRQLGFHQVISTGVAWNGDRFDGTLTTPNRRGEEKAIRL
                     RELVAERKDGETFAYGNSASDLPHLRIARHGLLVNGSLAARRDAAAMGVPTAEWT"
     gene            15525..>16033
                     /locus_tag="VEW08_17935"
     CDS             15525..>16033
                     /locus_tag="VEW08_17935"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HYJ42677.1"
                     /translation="MAERFRFIPPLVGKALVIGLLIVLLLVPLAQVESLVGERVEMRQ
                     TAAMRVAESWGGAQTTAGVLLAIPVETTRVVVDQTAAGRETQRMEVERNVLYVLPDTL
                     KISASADPGFRAVGLYETPVYTANVQISGEFVNRDFAQLLTQKEGREVKWGEARLLVL
                     NSESRALRAV"
BASE COUNT         2785 a         5015 c         5309 g         2924 t
ORIGIN      
        1 cggacgcgaa atgagctccg ttccttttgg cggtggccgc atcggctcgc gaatcgatga
       61 ggcggtgaag ctcgagccga agaatccgcg cgtgcgcctg gtggaggccc tggccatgtt
      121 cgatcgcgcc ggcaagaacg ccgacgagca ggccgtcgca accaggaacc tgcgcgcagt
      181 caccgtgatg ttcgagcagg cgcgcgccgc ggcttcatcc acaccggaat ggggcgctgc
      241 cgaggcgtac gcattcctgg gccgcgcact gttcgaccag cgtgacgtgg tcggagcgcg
      301 tgaagcgctg gagcgttcgc tgctgatcgc gccggactac gcgttcgcgc gcaagctgat
      361 ggcgcaaatc accagatagg atcccgcgac gtccgccgca actccatgtt cggcgccgat
      421 tcgatgccga tttccgtcgt ggcgcgcaag cgcctgactt agtatgacgt cgtcatgaac
      481 tacccgcggc tttttgcccc tctcgatctg ggattcacgc gcctgccgaa ccgcatcgtc
      541 atgggttcga tgcacaccgg gctggaagat cgcgctcgcg attttccgaa actcgccgcc
      601 tattttgccg cgcgggcacg cggcggtgcg ggcctgctgg tcagcggcgg catcgcaccc
      661 aaccgtgccg gctgggccaa gccgttcgcg ggaaaactgt cggctccacg cgaggtcgcg
      721 cgccatcggc tcgtgaccga cgccgtacat gccgagggtg ggaagatctg catgcagatc
      781 ctgcacaccg ggcgctacgc ctaccacccg cttcccgtcg cgccgagtgc gctgcgcgcg
      841 ccgatcaacc gcttcaagcc ccgcgcattg tcgtccgacg gagtgcgtga ccagatcgcc
      901 gacttcgtcc gttgcgccga gctcgcccag caagcccatt acgatggcgt ggaaatcatg
      961 ggttcggagg ggtacttcat caatgaattc ctcgcgccgc gcaccaatct acgcaccgat
     1021 gagtggggcg gctcgctcga aaatcgcgcc aggctcgcgc tcgaagtcgt gcggcagacg
     1081 cgcgtcgcgg tgggaccgaa cttcatcatc atctttcgca tttcaggact cgacctggtc
     1141 gaaggaggtg gcaccggtgc ggaggtggaa tggctcgcct cgcagatgga agcggccggc
     1201 gcaacgatgc tcaacaccgg catcggctgg cacgaggcgc gcatcccgac cattgccggc
     1261 acggtgccgc gcggcgcttt cggctgggta tcggcgcgca tcaaggcggc gacgcgcctg
     1321 ccagtgatcg ccacgaatcg aatcaacgct cctgagggtg ccgaggcgtt gctcgccaac
     1381 ggtgatgcgg acctggtgtc gatggcgcgg ccattgctgg cggacccgga acttccgcgc
     1441 aaggccgccg aggggcgcga cagcgaaatc aacacctgca tcgcctgcaa ccagggctgc
     1501 ctcgacaagg tcttcgaaaa ccagcgtgcg acctgcctgg tgaatccgct ggcggcgtac
     1561 gagacagagc tgatcattgc gtccacgcgc gccatcaagc gcattgccgt ggtgggtgcg
     1621 gggcccgccg gccttgcctg cgccaccacg ctcgccgagc gcggtcatcg cgtcacactc
     1681 ttcgagcgcg cgtccgagat cggcgggcaa tttcgttacg cgcgagaagt gcccggcaag
     1741 gaagacttcc acgacacctt gcgctacttc gcgcggcgca tcgagctcac cggtgtggac
     1801 ctcaagctca ataccaccgc cgatgcatcg cgtctcgcca gcgaaggatt cgatgcctac
     1861 atcatttcca gtggcgtggc cgcacgcgtg cccgacatcg ccggcatcga ccatccgaaa
     1921 gtcatctcgt atcccgacct gctctcgggc gcccgccggg ctggggattc cgtggctatt
     1981 gtcggggccg gcgggatcgg tttcgacgtg gccacgtttc tcacgcacga ggcggacagc
     2041 aactacttcg aggagtgggg catcgatcgc acgctcacgc agcgcggcgg gctagtgccg
     2101 ccgcgaccgg tggcttcgcg ccgccgcgtc tatctactgc agcgcaagcc gggcaggctc
     2161 ggcgcgacgc tgggcaagac caccggctgg atccatcgca gcgcactcag gcaccgcggc
     2221 gtcgtcatgc gcaacggcgt cagttaccag cgcatcgatg acgccggatt gcacatctcg
     2281 accgaggctg ggccggagct gctggccgtc gaacacgtgg tcatctgtgc ggggcaggag
     2341 tcggtcaatg ggctcgcgac cgagctcgcc gcaacggcca ggccggtgca cgtgatcggt
     2401 ggtgcgctgc ttgcggccga gctcgacgcg gagcgcgcga ttcgtgaggg tgtgatgctg
     2461 gcggcgaaga tatgagtggc ttcgacggta acaagaatgg ggacagtccc caattcgaga
     2521 tggcttttcc gacggccaat gacggcggtc gaattgggga cagtcccctc atccgcctga
     2581 gcgctcgcgc gcgcaggctg tccatccgcg tctatcccga tgcgcgcgtc gaagtggtcg
     2641 tgccgccgcg tgcccggcct cgcgaggtcg agctgttcat cgccacgcac cgggaatgga
     2701 tagacagcaa acgcgccgtg gcgctgcgca atcgccccga gccgcagccc tttccgccgg
     2761 caaccatcga actgcgggtg tcggctgaaa cctggcggtt acacgtcgca ggaggtgcgg
     2821 gccggttgcg gctggcggaa atgtccggcg gaatccgcga gcgtgtgctg cgcgtgaccg
     2881 gtgcgccctc gagcgacaaa ctgcgtgccg ccctgcgcag ctggctgtta cgcacggcgc
     2941 agcgtctgct ggccccgcgc gtcgccgctc tggccgctgc caccggggtt agctattcgc
     3001 agattgccat ccggcgccag cgttcgcgct ggggtagttg ctccgcgcgg ggtaccatca
     3061 gcctcaactg ctgcctgctg tttcaacggc ctgaagtcgt ggactacctc atcgtccatg
     3121 agctcatgca cgtgaagcac atgaatcact cggcgcgttt ctggcaggcg gtggagcgac
     3181 attgcgccga ctggcgtgcg ctcgatcgcg agctcgtgca gggctggcgt catgtaccgc
     3241 gctgggtttt ctccgaagga agttagggtc tcgatgggcg acaacaagat cgatctgaag
     3301 aacgaaggtg tgcactggga cctgggaact tcccagtcct atggcgagta cctgcagctc
     3361 gacaagctgt tggacgctca aaaacccaac tccatcgagc aggacgagat gttgttcatc
     3421 atcgtccacc aggtgtccga gctgtggatg cgcctgttcc tgcacgagct cgatcacgtg
     3481 ttcgaatgcg ttcgtcgtga caacctcgac ccgtcgttca agatgctggc gcgcatctcg
     3541 cggctgcagg aagaactgct gggcgcgtgg agcgtgttgt cgacgctgac gccgttcgaa
     3601 tactcctcat ttcgcaatac gctcggccgc tcatccggat tccagtcgct gcagtaccgg
     3661 ctgctcgaat tcaaactcgg caacaagaac cgcgacgtgc tcgcggtgca taagcgcgac
     3721 cctgccggat tcgaaaggct cgagcttgcg ctcaactcgc catccatcta cgacgaagtg
     3781 cttcgcctgc tgtcccgccg cggctacggc attcccgatg agctcatcga gcgtgatttc
     3841 tccgagccgt atgtcgcatc gaaggcagtc accgccgctt ggctgggcgt ctaccacaac
     3901 gcggaaaagg actgggatct gtacgaaatg gccgaacggc tcaccgatct cgaccagaaa
     3961 ttccagctct ggcggttcca tcacctgaag accgtcgaac gcgtgatcgg ctacaagccg
     4021 ggtacgggcg gaaccggcgg tgtgtcctac ctggccaagg ccctcgagct caaattcttt
     4081 ccggagctct ggcacgtgcg cacgtcgatg tgagcgcgct ggtggccggg ctggagctgg
     4141 atcacatcat tttcttttgc gctccggcgg cgccggaagc ggaggcgctg ctggcgcgag
     4201 gtctgcacga aggacccggc aattcacacc cggggcaggg caccgtcaat cggcgtttct
     4261 tctttcgcaa cgtctacctc gagcttctct gggtcgagga tttcgacgaa gcccaggcgc
     4321 cggacgcgcg gcggacaggg ctctgggacc gttggatcaa tcgccacaat ggcagctgcc
     4381 cgattggtct cgtattccgg ccggcagccc ctgatacgcc aacgccgttt ccgtcgtggt
     4441 cgtaccggcc aaggtatttt ccctccggat tttccatcga ggtcgcgctc gacattccgc
     4501 ccaacgagcc gctgttgttc tatctaccgt tcgcgaggcc ggcgctggtc gaggacgtcg
     4561 tgcaggtgcc gggcggcgcg cagatcggcc cagtcgacgg gacgacgctg cacctgccgg
     4621 agacgagcgg tctttccgct gccttgaatt cgctggtcgc ggcgggggtg gtgtcggtcg
     4681 agccggggcg ggaagttctg gtcgatctgc atcaccagaa tacgtccagt gaaatcatcg
     4741 acttgcgacc taaacttcca ctgaggttcc tcccggccgg cgcacggcgc cattccgcac
     4801 actgatcggt attgcgcgtt gatgctcgat tgatgcgcgg atatgatcgg cggcgcgcgg
     4861 aacccatcag aagataagga aggatccaat gaagagggga atggtttcgg ccgcggcttc
     4921 cgcgtgcctg ttgattgcct acgcgtcgct ggcggaggtc gccccgccca caccgaaata
     4981 ccaggtcgag aatctcgggc gcggccttct ggccctgcgc accggcgaca gttccgtcta
     5041 tgtgggctgg cgtttgttag gtaccgatcc tgcgggactg gccttcaatc tttatcgtca
     5101 gacgggagac gccgcgccgg tgcgtctcaa cgaatcacca ctgctcgcga ccacggattt
     5161 cgtggacacc accgcggacc tgacgctggg caacgcctat agcgtgcggc cggtgttgca
     5221 gggcgtggaa ctcacggcaa gcggcgcatt cagcctgccg gccaacgcac cgactcagca
     5281 gtacctcacc gtgcccttgc aacggcccgc gggcggcaac gtggaagtgc ctccgggcca
     5341 cgtcacccag gctgtcacct acagtccgaa cgacgccagc gtcgccgatc tcgatggcga
     5401 cggcgaatac gagatcgtgc tcaagtggga tccatcgaac gcgcgtgaca atgcctcggc
     5461 gggcctggcc gggcgccagc tcctcgacgc ctaccggctc gacggcacct tgctctggcg
     5521 catcgacctc ggccgcaaca tccgcgccgg cgcgcactac acgcagttca tcgttgccga
     5581 tctcgatggc gacggcaaag ccgaagtcgc ctgcaagact gccgacggca ccgttgacgg
     5641 cgccggcacg gtgatcggcg atgccaccag ggactaccgt tcgctgctgg tacccaccga
     5701 cggcatccag gtgccggcca ccaacgatgc gcgttacggc aaggtgctgg cgggtccgga
     5761 gttcttcacg gtgttcaatg ggctcaccgg cgcggcgctc gcgaccacta actaccttcc
     5821 cgggcgggat cccatcggtg gctggggtgg catcggcggc aatggcaaca gcgacaccaa
     5881 cggcaaccgc ggcgatcgtt tcctcgcggg gctggcctat ctcgacggca agctgccgag
     5941 cgtgatcatg gcgcgcggct actacggccg ctcggtgatc gcggcctggg actaccgcga
     6001 cggccagttg acttcacgct gggtgttcga ctccggcgtt ccaacagcca cgggttttcc
     6061 atgggcgggt tcctcgccgt tctcggggca aggcaaccac aacctgtcgg tggccgatgt
     6121 ggatgccgat ggcaaacagg aaatcatcta cggctcgatg gtggtcgacg acaacggagt
     6181 cggtctgttc tcgacgggac tgcggcacgg cgatgcgctg cacgcgggtg acctggtgcc
     6241 atcgcgtccg ggcctcgagg tctacggcgt gcacgagagc gagggcaaca cgctgtcgct
     6301 gggaacgccg ggcatggcgc tgtacgacgc gcgcgatgga cagatcatct ggagtctctt
     6361 gccggggcgc gacgtcggtc gcggcatggc ctccgacatc gatccgcgca cgccgggcta
     6421 cgagttctgg ggcgcgacgg aagtgggtct gctggatggg cagggtaatc gcgtcgccga
     6481 ttcgccgagt acggtgaatc atgcggtctg gtgggacgcg gacccgctgc gcgaactcga
     6541 ggatgccaac tgggtcagca agtgggactg gaactcctcg acactcacgc gattgctcac
     6601 cgccgacggt gccacctcga acaatggcac caagcagaac gcggcgatca ccggcgacat
     6661 tctcggtgac tggcgcgaag aggtggtctt ccgctcggcc gacaatcttt cattgcggat
     6721 ctactccacg agcattcccg ccaccaatcg gctgttcacg ctgatgcacg atccgcagta
     6781 ccgcacggcc atcgcgtggc agaacgtggg ctacaaccag ccgccacatc cttcattctt
     6841 catcggcgat ggaatggcgg aaccgtccgc acccccggtg tggcatctgg atacggatgc
     6901 gccggcgttt cgcaagttgt cgccgtcgct gcgctccctg ccgccgctgg gcatcctgct
     6961 gccggtgttc atcaaggccg aactggtgga cctcctggac gacaaggcca gcgcgcgcat
     7021 cgtgaacgtg accagcagtg gcgatgacga caagcggcgc cggcacgagc tgccggacgt
     7081 ggtgatcctc gacgatctcc tcgtgctgct gcgcgcggaa cgtgacggac gtgcagatcg
     7141 tgtctacacc atcgacgtgg aaggcgtgga cgcctccggc aacacggtag tggagtcggt
     7201 cgaggtgaca gtgacccgga actggtttcc ctggtaaccc ctcaaatgaa gcggggccgg
     7261 taaaccggcc ccgcctcccg ttcgtctgcg cgaactatac cgaccgaacc tgactattcc
     7321 tggttgaccc agaagttgat ggacatcgag ctcgccgagc tggccgtgct gccaatctgc
     7381 gcgggataac cgtccaccca cggaatgtcg taattgttgg cccagatgta gccgttcgcc
     7441 cagatgtaac cattggccca aatgtaaccg ttggcccaga tgtagccatt cgcccagatg
     7501 tagccgttgt tccagatata tccctgctcg ttcaacgtgc cgctgggcgt gctgaccgtg
     7561 aacgtgccgt cggccttctg gcgcacgctg ccctggtagt gttgcgtgcc ggcgaggtcg
     7621 gcgttgatgt tgagaccctg gttggcgcag ttgctggccg tgccgtacac ggcgcgctct
     7681 gcgtccacca tgccggtgcc ctgctggagc acgctgtagg ccagtttgcc gttggcgtcc
     7741 accgccggtt tggcgctcgc gatgatgcgg cacttcacct ggtcgggcgt gaggctgggg
     7801 ttctggtgca gcaccagcgc cgcgacgccc gacaccactg ccgcggcctg cgaagtgccg
     7861 gacatggtga agaagtcgcc gttgttcatg taggtcggat gatcgaccgc gatcttcatg
     7921 tacgtgccca tgaagcccca ggcgtgaccg ccgggtgcga ccaggtccgg tttcacgaaa
     7981 ccttcgtacg tggggccggt ggacgagaac gaagccagcc ggtcgtcact cttgttagtg
     8041 gtgaagttgt cggtcatcgc gccgaccgtg atgacgtagg gcacgttgcc cggaacaccc
     8101 acggtctgcg gcagcggtcc gctattgccc gctgacacga caacgacaat gccggattgc
     8161 caggcgcgca tcacggcttt attgagcgga tcgtcccaat agcgcgattg cgcctgggcc
     8221 cctagcgaca gattgatgat tcgaatggcc agcgggttct tgttggtgat cacccagtcg
     8281 atggcgcgga taacggtggc atagttgctc gacccgtctt cgccaaaggc cttgatcgag
     8341 atgatgcgcg catcgggcgc aacgccgaaa tattcgccgg tcgacttctt gcggctgctg
     8401 gcgatgaggc tggtgacgtg cgtgccatga ccggtggtgt cggtggaaac cggcgcccag
     8461 ttgctgacca cggtgttgct gaccgcgtcg tactgcacgg ccatccggcc gagcccgttc
     8521 gaatccacgt cgagcgaatc gattttccag tagccggtgt cgatgaccgc cacgccgatt
     8581 tcgcgaccgc gaatgccggc gctatggagc gctttcgcat tcacgtccac gggcgtcgga
     8641 tcgccttcac cgtaggtcgt gttgtaggag atttcgacgt cgtcgagatc gacgtagtcg
     8701 ccggggaatc cttcgctcat cgagctcacg aaacggatcg cggtgttgcg gccgcgatag
     8761 gcagtgatgt tgtagctcct ggcggtgaaa ccggtgtcgt tgcccgcgcc cgagatcctg
     8821 ccgacctcgg tccacgtgga gccgccgttg gcgctggcct gcaccgcgac gtattcgccg
     8881 gcttcgaggc tggcgcgtga atagttgagc ttgagcgtga cggagctggc gctcgacggc
     8941 gtcgcggcgc ggcgatagac cgcgccattg ctgccgatga tcatgcggtt gccgccgcgc
     9001 tcgagccagc cgatgaccac cttgccgttg tacggcgagt tgttgtcgtt ctgttccacc
     9061 cagtcgccat accagcggtg cgaaccgttg ttgttggcga atgagccggt ttcgaaattg
     9121 tcgcgtacgt aagcggcggc cagcgtcgtg actggagcat tcggtgtgac ttgcttgacg
     9181 ccctgaatgg agagcacggc ctggtgctgg gaggaggtga gagaggcttc gacggcgtcg
     9241 atgaccccga ggcgcgaaac gactttgccg ccggcggcct tcacggcacg ggcagcattg
     9301 tcggtgctgt cggcttcgac gatgtagctg tgtgcctcgc cggcgtcggc aacgagggtc
     9361 gttccggacg tgcccgaagg cgcggcagag cacgatacga gcccggccag cgcaaccgtt
     9421 cccaggactc gagcagcaat ctcaattaca cgtacggaca tcaacgcctc ttcggtggcc
     9481 ccagtctgct tttgcagaca gcgccattag aggtgcgaac ggcaatttcc tagcgtgcgt
     9541 gtgtgacgtg gctctcggga aaaatgtgac ggacgtctga taagggccgg ttaaatccgt
     9601 gccctatgtc acatgcaatc agcgctgggc tgtgcgttga acagaagctg gcgcttcctt
     9661 gccctggccg aggattttgt ccaacaggtt cgagagtggc gtcgccgcac agaaaagatg
     9721 gccctgtccc tcgtggcact gttcggcgat gaggaactgt gcctgggctt cggtctcgat
     9781 gccttcggca acgatgcgca gcccgaatgc atggccgagc gcgatggccg cgcggcagac
     9841 ggcccgcgct tcgtggtcgt gaccgacttc ttcgacgaac gaacggtcga tcatgatgcc
     9901 gtcgagcggc gagctgcgta gcttgccgag ggaaatgtag ccggtgccga agtcatcgat
     9961 gagcacgcgc acgccctggc cgcgcagctg atgcagggcc gcgaggccgg tacgacccgt
    10021 ggacagcatt tgttcggtaa tttccaggtc gagctgcgaa ggctcgatgc cggcttcgcg
    10081 cgccgaattg gtgacgtcct cagcgagact ctcggcggac agatactggc ggccgacgtt
    10141 gaccgagacg cgcagtgccg ggtaaccacg ctcggccagt acggcgagat cgcggcaggc
    10201 gcgacgcaat acccagcggc cgagttgtac cgacaagccg gacagctcgg cgatgggcag
    10261 gaactccgac gtgggcacca gtccacgaat cttgtgcggc cagcgcagga acgcttcgac
    10321 cgcgacgatc tcgcgtgtcg agagaaggaa gcgcggctga tagtgcagct cgagttcgtc
    10381 tttctggatt gcctgggaga gttcctggct ccagtcgagg cgcgtgagcg acgccaggcg
    10441 cagcgtgtcg gaatagaacg tgaccaggcg gtcgatgccc tggcgccggc cggcatcgag
    10501 cgtggtgagc gcgcgttcga tgagctcgct ggcgcggtcg ccgtcggttc ccgaaagcgt
    10561 cacaccggat tgcggcgcga gctggaagat ctgtccgtcg agatcaaccg ggtgctgcaa
    10621 agccttcatg atggcttcca cggccacatc cgcgtcggcg cgcgcgcgga tgtccgtcag
    10681 cacgacgccg atgacgtcgt cggaaacgcg tgcgatgcgc ggcagactgg tgcgcgcttc
    10741 cggaagagtc tcggcgatcg tcttggccgc gcattcgagc aggcgattgg caattccgga
    10801 gtcgagcaca cgttgatagg tggcgtagcc accgatgtga atgagtacga cacccacggt
    10861 gtcttcgcgc agcgtggcca tgtcgagcgc catgcggagg gatttttcga agccgtcacg
    10921 atgcggcagc atggcgctgg cgctctgcgg atacaggccg gaatcgttgg ccgtgacgcc
    10981 ttcgcccagg tcacggcaga cgacgagtac gcggtcgaag ccctgcacct ggaagcgcat
    11041 ctccagtttc aggtccccgt cctggtagcg ctgctgcgcg ggtttgcgtg tcttgaggat
    11101 tctgcgcacg gtctgcaacg cgtgcgaagc aaccgattcg ggccacagcg tgtcaagagt
    11161 gcgcccggga agggcttctt cctgggcatc gaggccgcgc accgcctggc cgccaacgca
    11221 ggaaaggatc acgccatcac gccgcacgat cagtgcgaga tcgggaagtg accgggctag
    11281 agcatctcga atatcgctgt ccatgctgaa ttcattatgt cagcgcgctt cgcgcagaac
    11341 tcgtagcgcg ttcacagtgt cgtcgcttcc acgcgtcgac atctgctcaa tgatgacgcc
    11401 gttggcgatc aggctgtcga cattcgactc gaccgcggta atgactgact gcagtgacga
    11461 cacgtgttcg gtgccaaacc agccgcgcca ggcgttgcgg ccgcgctttg cgcgatacaa
    11521 cgcgaggtcg gcgatcttga tggcctgctc ccagctcacc gactcagggt ttcccggtac
    11581 aaacggcagg caggtgaggc cgatggagca actggtgcgc acgacggcgc cacgcggcag
    11641 cacgcacttc atcttggcga tgcgttcgcg cacgcgctcg gcaagcatgc aggcgtgctc
    11701 gacgtgttgg gcctgcgcga cgacgacgaa ctcatcgccg ccccagcgcg ccaggacgtc
    11761 gtcaggacgg atcgcctgtc gcaacgtctt ggcgatctcg acgatgacat cgtcgccggc
    11821 ggcgtggccg tgctggtcgt tgatgggctt cagcgcatcg agatcgatga tcatgatggc
    11881 gaggccggag tttcccttgc gatcggcgat caggcggggg aactgctgca tgagatagcg
    11941 gcgattcgca agaccggtga gcggatcgct gaagctcgcc atttccaggc gacggttcgc
    12001 gtcttcgagt tcacggtttc gttgggccag ctcgtacgtg cggtcactga cctgttgttc
    12061 gagttccagc cgttgcatgg cagcgcgcgc gatgcggcgc tgctgggcgt accagacggt
    12121 aagcagcatc agcaccgcga gcatcgcgta accgagatag gcgaccggcg agagccaggg
    12181 agacggtgcg acgtcgatcg gcagggcgag atcctgcgtg ctccagactc cgtcgctgtt
    12241 gctggcgcgc acgcggaaca cgtactggcc gccgggcagg ttggtgtaag tcgcgacgcg
    12301 gcgttcgtcc gcgcgcaccc agtcgctgtc gaagccgtcg agcttgtatt cgtagcggtt
    12361 cgagcgcggg tcggcgaagt cgagcgcggc gaacttcagc gtgatgacat cttccttgtg
    12421 tccgagattg agccgctcga tgcgctcttc gggtacgccg acgatccccg gagtattgag
    12481 cttcaggaat tgtgtgagca ccacgcgcgg cgggcgctca ttgaattcga gcacttccgg
    12541 gtagaacgtg ttgaagccgg cggcgccacc gaagaacaac ttgccgctgc ggtcacggta
    12601 gtgcgagcca aaattgaact cttcggcctg caggccgtgc agccggtgaa agcgctggat
    12661 gacaccgctc ttgggatcga ggcgcgccag gccgaagttg gtgctgatcc agatatcgcc
    12721 ggtgccatcg gcgcgcaggc cgtataccga attgttgggc agtccctgcg cctcggagaa
    12781 attggcgaac cgcaggccgt ggggcgcgtc ggccggattg agcacgcggt cgagaccgcc
    12841 gccgcgcgtt ccgatccaca caccgccggt atcgtcgacc aggatcgaat agatagtgtc
    12901 ggcgctcaaa gtgtcgagct gcttggcgcc gcgcttgtaa tagtagagac gccgggtctt
    12961 ctggtcccag acgttcagcc cgccgccatc ggtgccgatc cacacgtggc cggagcgatc
    13021 acgggcgagc gcggtgacgc gtccactcga caggtgcagg ccatcttcgg gacccgggcg
    13081 cagattctcg aagcggcgag tgcgcgcatc gaagcgcgag atgccaccgc cgaacgtgcc
    13141 cacccagagt tgtttgtcca cctcgagcag gctcatcacg cccgcagcgc ccagcgacgt
    13201 gggaacagcc gggtcgtgca ggaatacctc ggccttcagt gtccgcgcgt cgaagcggtt
    13261 caggccaccg cccatcgtac ccacccacat gtcgccattg ctgccttcga gcatcgacat
    13321 gacgcgctcg tcgctcaggg aagagtgcac accctgcacg tgacggatcg tgctcacgcg
    13381 ccccgagctg cgatcgagca ggttgacgcc gctgccaaag gtgcctatcc acaggcgccc
    13441 cagcttgtct tcagcgaacg aggtgatgtt gtgatcgcta taaccttcct cggcgctcgc
    13501 gcgcacgtgg ccaaacgacc aggtccgggg gttccacttg gcgaggccgc cgaatttggt
    13561 gcctatccac agtgagccac cccggtcttc ggccagcgag acgatgtagt cgtcgggcag
    13621 cgagcgggcc tcggcctcgt cgcggcggta gctggagaag atttcggtag ccgaatcgaa
    13681 ccaggacaag ccgcgcatcg tgcccaccca aaggcggccc tggctgtcga tgagcaggga
    13741 ttgaatgcga ttgtccggca gggccgcgct gcggcccggc gcgtgctcgg gaagttcgtg
    13801 atagacacgc cagcgggagt tacgcggatc gaagcgcgca agtcccatgt ccgtgccgac
    13861 ccacaccatg ccgtcgggcg tctcggcgag cgcgcgtacg cgcagcggtt ggcccgcggc
    13921 gacgagttcc gtcggaagtg cgatacgcgt gacgtcgaga ttcgccgtgg tcaggcgatc
    13981 gagcccgctg gcggtgccga ccagcgtgtc gccgttgcgc agattgacga gactgaagac
    14041 ggaattgctc gagagcgaat tcggcaacgc ggatgaatga cggaggcggc gcagttcgtt
    14101 gctgtgcgcg tcgaagatgg cgagtccctc atcgcgagtc gcgatccaca accgtccgag
    14161 gcggtcgcgg gccagcgtgc gtacgcgctg caggtcgggc gcgtcgcgca atatcggcgg
    14221 cgcattgagc ttgccggtga cggcattgcg gaagaccacg ccggcgccgt cggtcgcaat
    14281 ccacaaaccg tcttcgctcg cgaccagcga ggaaatccag ccgttcggca gcgtctcgct
    14341 gtcgccgcga tcgtggcgca gctgccggaa atcataaccg tcgaatcggt tgagtccgtc
    14401 ctcggtgcca aaccacatga agccttcgct gtcctggacg atggcattga cggcttgctg
    14461 tgacaggcct tcttcgaggc tgtagcgctg gacgctgagt ggaggagtgg cgagtgcgac
    14521 ggtcgaaaaa atgcaggcgg ccgcggcggc ggcgacgcgc gcgcgcattc cgaagggccg
    14581 gttttttccc cgtgttgttg acattgtcca gaagctacgg acgactccag ccgtcatcgt
    14641 agttccgcag gcgcgcgagc tatgtgactg gtgtcggatg ggcccgtaca ggagcaccat
    14701 gtaggagcgc taccacgtga tatctgtgat gtggatcaca ggcgcgccgc accttaaggt
    14761 gcagttctaa gtccattcag ccgtgggcac acccatcgcc gccgcgtccc ggcgagcggc
    14821 gaggctgccg ttcacgagca gtccatgtct tgcgattcgc aggtgtggca gatccgaggc
    14881 gctgtttccg tacgcgaaag tttcgccatc ctttcgttcc gcgaccagtt cgcgcagtcg
    14941 gatcgccttc tcctcgccgc gccggttggg agtcgtcaga gtcccgtcga accggtcgcc
    15001 gttccatgcg acgcccgtgg agatgacctg gtgaaaaccg agctggcgac cgaactccgg
    15061 cacgtagaaa tcgacactcg cggacatcag caccatgtgg tgcccggcat cgtgatgacg
    15121 gcggatcgcg gccagcgcgt cggcgaagct acgtcgcgcg atcgtctcgg tgacgaaggc
    15181 tgccgcggcg gcgtgcagtt cggcgcgcgg agtgccgcgc agcgttgcac gcagcaacga
    15241 ttgcttgagc gcagcccgat cgtggtcaaa cgcgaagcga atggtcgcgg gcaacacctt
    15301 gagcaatcgc aacagccgcc atgggcgtgg ctttgcgagc tgacgcagca ccagcggaaa
    15361 cagcgtgtcg cgatgggtga tggtgccgtc gaggtcgaag atggcgatgg gcatgccagc
    15421 atgttaacga aggccttgcc acgatttgcc acaattgcgc cgcgccattg gccacattcg
    15481 ccaggacact ccccgcactg caacctgcat caagggagat cgtgatggcc gagagattca
    15541 gattcatacc gccgctggtc ggaaaagcgc tggtgatcgg cctcctgatc gtgttgttgc
    15601 tcgttccgct cgcccaggtg gaaagcctcg tgggcgaacg agtcgaaatg cggcagaccg
    15661 ccgcgatgcg cgtggccgaa tcctggggtg gcgcgcagac caccgccgga gtcttgctgg
    15721 cgattcccgt ggagaccacg cgcgtggtcg tcgaccagac cgcggcaggg cgagaaaccc
    15781 agcgcatgga ggtcgagcgc aatgtcctct acgtgctgcc cgatacgctg aagatcagcg
    15841 ccagcgccga cccgggtttt cgcgccgtcg gactttacga gacaccggtg tacacggcca
    15901 acgtgcagat ctccggcgag ttcgtgaacc gcgacttcgc ccaactactg acgcagaagg
    15961 agggccgcga ggtgaaatgg ggcgaggcgc gcctgctcgt gctcaactcc gaatcgcgcg
    16021 cattgcgcgc cgt
//
LOCUS       DASQJS010000453         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1552241, whole genome shotgun sequence.
ACCESSION   DASQJS010000453 DASQJS010000000
VERSION     DASQJS010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552241"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1993
                     /locus_tag="VEW08_17940"
     CDS             131..1993
                     /locus_tag="VEW08_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42678.1"
                     /translation="MSIEGQWLRHPGGRILLFDEAPGEFEFEFELDKHSPKTPPPSNA
                     LKAGDVNVASIDLPARDSLLRLAAVPKTRKAAVELLDHVKAGRIDGIRCLRSTAAMAR
                     VKRLGYDPADAIRAGNDAVVLLDPTDLRGGKPMIAIRHELDPRCGSAGASPPTSAAIA
                     KFDAALLKSLASFSAWREGISLGRSAKCALAPLDDPGRTGLGNVLPRLIRLPYAMPAA
                     PGAPKLVRHRHTSYKCNSAFSFVPDGTPVDLLPANMRPHFVAPDDRFMIVWELQNQLH
                     ALLKEDAFKDLVNRSRTAATLKAEESDRLHLAVVNLSGRGNLCEPGLAHYGAQFNEWG
                     GSTPKILIVYAAHQLHFDLEQMVAADRIRDFATLEKKAAEEWKVFSCKPRLELFKFTA
                     PATSSGLLKVEMSVALNTLLREMTANTGYSTARASTLILKIGFEYLASLAWQSGLRHP
                     TRGGIWYGTTYCTDQKPDATDLACYGKDSETKIACPSTTPRVFWKADPMKIYGVRGSA
                     LSFATYMTLLAQGRLVDATNSARIEALLKNACGVAKDAGVLGIRAQKCAWTDTTLHDA
                     VLIEREVVRGARRRTLRYVIAFTCRLKNKGDPALGTMHALIRRIDTIMEERALA"
     gene            1990..>2782
                     /locus_tag="VEW08_17945"
     CDS             1990..>2782
                     /locus_tag="VEW08_17945"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ42679.1"
                     /translation="MIIDCHCHAGTGDGLTGPWDTRASLTAYLRRASAAGITHTNLFA
                     AFHTDYARANHEVSRIVASRPGQFYGFAFVNAVRDRGRVRQLVQTAVQRYGFVGIKVH
                     RHEGRITREICEVARAFGLPVLYDVMGEVSACELLAREYPDVPFIIPHLASFAGDWRA
                     HLALIDQLVRHRNIHADTSGVQRFDLLAEAVQRAGASKILFGTDGPWLHPAAELAKVR
                     ALQLPVGDERKILAQNFLRLIDGVVAQPRKTPRPISAVTATIAWQP"
BASE COUNT          540 a          895 c          858 g          489 t
ORIGIN      
        1 agcatggaac cccgccgaag aagcgccgct gggtactggc cttcatgtcg cagggcctca
       61 acaagcagga gcgccaggac ctgctccagc tggtgccggc ggtgcacgac atcatcgtga
      121 ggaacaaccc atgagcatcg aaggccaatg gttgcgccat cccggcggcc ggatcctgct
      181 gttcgatgaa gcgccgggtg agttcgaatt cgagttcgaa ctcgacaagc attcgcccaa
      241 gaccccgcca ccctccaacg ctctgaaggc cggtgacgtg aatgtggcga gcatcgacct
      301 gcccgcgcgg gactcgctgc tgcgcctcgc agccgttcca aagacgcgca aggctgctgt
      361 cgaactactc gatcacgtga aagccgggcg catcgatggc attcgctgtc tgcgctcgac
      421 ggccgcgatg gcgcgtgtca aacgcctcgg gtacgacccc gcagatgcca ttcgcgccgg
      481 caatgacgcg gtggtgttgc tcgacccgac ggatctgcgt ggcggcaagc ccatgatagc
      541 gatccgccac gaattggatc cccgctgcgg gagtgccggc gcgagcccac ccaccagcgc
      601 cgcgattgcg aagttcgatg cggctctgct caagtcgctc gcgagcttca gcgcctggcg
      661 cgaaggaata tcgctcggac gttcagccaa gtgcgcgctg gctccgctcg acgatcccgg
      721 cagaactggt ctcggcaatg tgctgccgcg actgattcgc ctcccgtacg ccatgcccgc
      781 ggcgccgggt gcacccaaac tcgtgcgcca tcggcacacc agctacaaat gcaattcagc
      841 cttcagcttc gtgcccgacg gaacgcccgt cgatctgctg ccagccaaca tgaggccgca
      901 tttcgtcgca ccggacgacc ggttcatgat cgtttgggag ctgcagaacc agctccacgc
      961 gcttctgaaa gaagacgcct tcaaggatct cgtcaaccgt tcgcgcacgg cggcgacgct
     1021 caaggcggag gaaagcgacc gcctgcacct cgccgtggtc aacctgagcg ggaggggcaa
     1081 tctttgtgag cccgggctgg ctcactacgg cgcgcaattc aatgagtggg gcggaagcac
     1141 gccgaagatc cttatcgtct acgcggcgca tcaactgcac ttcgatctcg agcagatggt
     1201 ggcggcagat cgcatccgtg acttcgccac gctcgagaag aaggcggccg aggaatggaa
     1261 ggtgttctcc tgcaagccac gcctcgagct attcaaattc acggcaccgg caaccagcag
     1321 tggattgctc aaggtggaaa tgagcgtcgc attgaacacg ctactgcgag agatgaccgc
     1381 caacaccggt tacagcactg cacgtgcatc aacactgatc ctcaagatcg gtttcgagta
     1441 ccttgcatcg cttgcctggc aatcgggcct gcgccatccc acgcgcggcg gcatctggta
     1501 tggcaccacc tactgcacgg atcagaagcc cgacgcgacg gacctggcgt gttacgggaa
     1561 agattcggag acaaaaattg cctgtccgtc gacgacgccc cgagtgttct ggaaagccga
     1621 tccgatgaag atctatggcg tcaggggtag tgcgctgtcc ttcgcgacct acatgacact
     1681 tctggcacaa ggccgattgg tcgatgccac caactccgca cgtatcgaag cgctgctgaa
     1741 aaatgcctgt ggcgtggcga aagacgcggg cgtgttgggg atacgagcac aaaagtgcgc
     1801 atggaccgat accacgcttc acgacgcagt cctcatcgag agggaggttg tcaggggcgc
     1861 ccggagacgg accctgcgct acgtgatcgc gttcacgtgt cgcctgaaga acaagggcga
     1921 tcccgcgctc ggaacgatgc acgcactcat ccggcggatc gacaccatca tggaagagcg
     1981 ggccctcgca tgatcatcga ctgtcactgc cacgcgggta cgggcgacgg cctcaccggt
     2041 ccctgggata cgcgcgcgtc actcacggcc tatctacgcc gtgcctctgc cgcgggcatc
     2101 acgcacacga acctgttcgc ggcgtttcac accgactatg cccgggccaa tcacgaagtg
     2161 tcgcgtatcg ttgccagccg tccggggcag ttctatggat tcgctttcgt gaacgcggtg
     2221 cgtgaccgtg gccgcgtgcg gcaactggtg cagaccgccg tgcagcgtta cggcttcgtc
     2281 ggcatcaagg tgcaccggca cgaggggcgc atcacacgcg agatctgcga agtggcgcgc
     2341 gcctttgggc tgccggtgct ttacgacgtc atgggcgaag tgtctgcctg tgaattgctg
     2401 gcgcgtgaat atcccgacgt gcctttcatc atcccgcacc tcgcgagctt cgccggggac
     2461 tggcgcgcgc acctggcgct catcgaccag ctggttcgcc atcgcaacat ccatgccgac
     2521 acatccggtg tgcagcgatt cgatctgctg gcggaagcag ttcagcgagc cggtgcgagc
     2581 aagatcctgt ttggcaccga cggaccctgg ctgcatccgg cggccgagct cgccaaagtg
     2641 cgtgccctgc aattgccggt gggcgatgag cgcaagatcc tcgcgcagaa tttcctgcgc
     2701 ctcatcgatg gcgtcgtcgc gcagccgcga aaaacgccgc gtccgatctc ggcggttacc
     2761 gcgacgattg cctggcaacc gg
//
LOCUS       DASQJS010000454         9141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2812770, whole genome shotgun sequence.
ACCESSION   DASQJS010000454 DASQJS010000000
VERSION     DASQJS010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2812770"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..987
                     /locus_tag="VEW08_17950"
     CDS             316..987
                     /locus_tag="VEW08_17950"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYJ42680.1"
                     /translation="MDIRVMVASEVRLYRDALQSVLRGVTGIALVGVASSAEEVIKQA
                     CMLAPSVAVVDMAMIESFSVAKPIARAPQVGGVVVLGIPEVPAEVIACLPSGILGFVS
                     RDGTVAVLLDAIRAAGRANVPQKAPNGACIDGLTVREMEILRLMQQGVSNKTISRHLG
                     IELSTVKNHVHSILAKLGVHRRGEAISLLYAHEKAARGWDVLPPVEGSDSAVDDDPSF
                     EAAMA"
     gene            1130..1654
                     /locus_tag="VEW08_17955"
     CDS             1130..1654
                     /locus_tag="VEW08_17955"
                     /inference="COORDINATES: protein motif:HMM:NF016834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lecithin retinol acyltransferase family protein"
                     /protein_id="HYJ42681.1"
                     /translation="MNYEIYCGPRRCVRPLAPGEAPPLGAHLAMPWLGYTHHGIYVGG
                     GNVVHYGALKYNLIRRPVEEVSLAAFAEGRPVYVVMHSEEAFEVAEIIRRARSRLGEA
                     RYRLFSNNCEHFVEWCLHDQPRSFEAETALEFPRWFGERIVVAMLRFARWAFRARGAV
                     PESARVEPRLPRRP"
     gene            complement(1719..2942)
                     /locus_tag="VEW08_17960"
     CDS             complement(1719..2942)
                     /locus_tag="VEW08_17960"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYJ42682.1"
                     /translation="MHANLRHLHTFREVARLGSVSAAARAVHISQPAVTQAIAGLERY
                     FGSPLLLRHNMGVSLTSAGEICLARVERSMGQLREALGAATRNAGTERLDVERLVRSR
                     QLEALGAVVEHGNFSIAARARHVSQPSIHRSARELERLLGVQLFEKTSFGITPTREAA
                     ELARRARLAFAEIEQARAEVHALSGGESGRTVISALPLAGSYLLPATLLEFTQKHPRH
                     GVAIIDGTYEHLLSGLRAGESDILIGAMRDPAPAADVIQEHLFDDPLAIVVRAQHPLA
                     QRPRVAAAALGQFQWIAPRTGSPLRVHFDALLASTGHQLPSPPLECNSMAAARAFLLE
                     SDHVMLLSAHQIHYEMQAGLLVALPHPAGDVVRPIGLTLRRNWRPTTTQQQLLDILRR
                     SARAAAASHSGAGPD"
     gene            3062..3736
                     /locus_tag="VEW08_17965"
     CDS             3062..3736
                     /locus_tag="VEW08_17965"
                     /inference="COORDINATES: protein motif:HMM:NF006731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HYJ42683.1"
                     /translation="MVTNVERVERGVVEALAPFGVATIHEAQGPYGLMAPYMRPIYPG
                     ALIAGTAITVSVPPGDNWMIHVAVEQCRPGDILVVAPTSASDCGYFGDLLASSLKAQG
                     VLGLIIEAGVRDVRALTEMRFPVWSRYVSAKGTVKATLGSVNIPIICAGAQVNPGDFV
                     VADDDGVCVVPRARVDSVLASARARERKEAAVRARLVTGELGLDVYEMREALAAKGLT
                     YRDGKD"
     gene            3739..4821
                     /locus_tag="VEW08_17970"
     CDS             3739..4821
                     /locus_tag="VEW08_17970"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF016210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HYJ42684.1"
                     /translation="MLTGIPCTLMRGGTSKGLYFNAADLPPDRELRDRVLLAAMGSPD
                     ARQIDGAGGAHPLTSKVAVISRSAHPGADVDYLFLQVVVDRAEVSDSQNCGNILAGVG
                     PWAIENGLVPVAGPTTTVRIHMVNTGSLAVAHVPTPHGAVEYEGEARIDGVPGTAAPI
                     ALEFLDVAGSSSGSLLPTGAVTDTFEGIEVTCIDNGMPVIIVRAADLGVTGAESPAQL
                     EANAALVARVERMRLSLGPRMNLGDVTRKTVPKISLVSAPRHGGSIGTATFIPHRVHE
                     AIGVLGAVSVATACAIPGSVAAQVAQRRDNARRIEVEHPSGFFTVDVEVERGDSFVVR
                     GAALLRTARKILRGEVFVPGAVWSHA"
     gene            4818..5699
                     /locus_tag="VEW08_17975"
     CDS             4818..5699
                     /locus_tag="VEW08_17975"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HYJ42685.1"
                     /translation="MTAPSVCLLGFGEVGQVLCEDLRTRVGHLSAWDLKFSDSASEPR
                     RAAARHEGLAASDARHAVDGADLVISAVTAAQIVEAASSAAPHLKRNAYFFDLNSSSP
                     AAKRQAAGIVESAGGRFVEAAIMSPISPRRAASPMLLGGAHADGFLPLAREFGFSGAE
                     VFSGKLGAASAAKMCRSVVVKGLEALMLESLLAARQHGVEGAVLESLRSLFPPDSSAA
                     TPRYMISRALVHGRRRAEEMGEAARTVAEAGVSSRMSSACAEWQEWASHHPDAAATPD
                     IDVMLDTLLSARGSAPC"
     gene            5693..6718
                     /locus_tag="VEW08_17980"
     CDS             5693..6718
                     /locus_tag="VEW08_17980"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ42686.1"
                     /translation="MLIIDCHGHYTTAPEPHQKFRDAQLVRIKDSTLPAPVPAQVSDD
                     EIRESIEANQLRLQRERGADLTLFSPRASAMGHHVGDESVSTSWTRACNDLVKRVVDL
                     YPENFAGVCQLPQSPGVPISHSVDELTRCVRDLGFVGCNLNPDPSGGHWNSPPLTDRH
                     WYPFYEKMVELDVPAMVHVSASCNPNFHATGAHYINADTTAFMQFIQGDLFRDFPTLR
                     FVIPHGGGAVPYHWGRYRGLADMLKRPALDGHVMRNVFFDTCVYHQPGIDLLVKVIDI
                     DNILFGSEMLGAVRGIDPQTGHHFDDTRRYIDALAISADARRQIFELNARRVYPRLDV
                     MLKARGL"
     gene            6715..7605
                     /locus_tag="VEW08_17985"
     CDS             6715..7605
                     /locus_tag="VEW08_17985"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYJ42687.1"
                     /translation="MNAGHFQPDPGWLSFHAAPGKPTFRPPPGAVDAHCHVFGPGAVF
                     PYAPERKYTPVDAPKETLWALRDFLGFERNVIVQGTCHGTDNRALVDALRNSDGRARG
                     VASVSRDVTDSELADLHDAGVRGVRFNFVKRLVDVTPPAVLHEIATRIAPLGWHVVVY
                     FEAHDLPELAPFIASLPTIVVVDHMGRPDVRLPVEGLEFERFVRFMREHSNVWSKVSG
                     AERLSLKGPPGYSDFVPFARRIVESFPGRVLWGTDWPHPNMKSHMPDDGDLVNLLPRI
                     APTPELQRFLLVDNPKRLYW"
     gene            7602..7979
                     /gene="ligA"
                     /locus_tag="VEW08_17990"
     CDS             7602..7979
                     /gene="ligA"
                     /locus_tag="VEW08_17990"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HYJ42688.1"
                     /translation="MKTYGDIPGTTVFDAEQSRKGYHLNMFCMSLMKAANRERFKSGE
                     RAYLDEWPMTDEQKQAVLDRDYNRMIALGGNIYFLAKIFATDGRSFQYAAALMTGMSQ
                     EDYARMMLDGGRAYVPGKPKEGV"
     gene            7979..8821
                     /locus_tag="VEW08_17995"
     CDS             7979..8821
                     /locus_tag="VEW08_17995"
                     /inference="COORDINATES: protein motif:HMM:NF009901.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HYJ42689.1"
                     /translation="MARISAGITCSHVPAIGAAIDLGKTKEPYWAPLFAGFEFSKQWM
                     AANTPDVVFLVYNDHATAFSLEIIPTFAIGCAPEFAPADEGWGARPVPVVRGAPELAA
                     HIAASVIESDFDLTLVNKMDVDHGLTVPLSLMFGQPQRWPCLVIPFAVNVVQYPPPSG
                     NRCYQIGKAIRRAIDSFDQDLNVQVWGTGGMSHQLQGPRAGLINKEFDAKFLDGMIKD
                     PEALARIPREQYLREAGSEGIELVMWLIMRGALEGPVREVHRFYHVPASNTAVGHLIV
                     EPQS"
     gene            8818..>9141
                     /locus_tag="VEW08_18000"
     CDS             8818..>9141
                     /locus_tag="VEW08_18000"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYJ42690.1"
                     /translation="MSKSMTQPLKLVVAGVGAFGQKHLDACKLIEGVEVESVVGRQLA
                     PTQEVARKYGVAHATTDLAEALARPGVDAVILATPTQMHAAQSVQCLNAGKHVQVEIP
                     LADSLK"
BASE COUNT         1543 a         2933 c         3001 g         1664 t
ORIGIN      
        1 atggtccaga tgtagacaga tcgacgaagg gaatagatcg tttgatctag aaaccgcgag
       61 tgcgcaaagc cgacataagg cgaaactttg ctcgcaagca acgcgcgacg ccgcgcttgt
      121 gggttttccc acgcacgctg ccgcaaatcg tgttgcgtcc taacttctcg acactttttg
      181 tgttgacaag ttgactgggt ctaagtgatc gtcatgcgcc atcggcctag ggagaggttg
      241 ggtttccgac gtacaactcg gggtcttggg ggaccacgca aggattgcgc ggccattcgg
      301 ccagggagta gcgcgatgga cattcgcgtg atggttgcta gtgaggtacg gctctatcgc
      361 gatgcgctgc agagtgtgtt gcgaggtgtc accggcattg cgctggtcgg tgttgcttct
      421 tccgccgaag aagtcatcaa gcaggcatgc atgctcgctc ccagcgtcgc cgtcgtcgac
      481 atggcgatga tcgaaagttt ctccgtcgcg aagccgattg cgcgcgcacc gcaggttggt
      541 ggagtcgtgg tgctcggaat tccggaagtt ccggccgaag ttatcgcctg cctgccgtcc
      601 ggcatcctcg ggttcgtctc gcgcgatggc accgtcgcgg tattactcga tgcgattcgt
      661 gcggcggggc gtgccaacgt tccgcaaaaa gccccgaacg gcgcgtgcat cgatgggctc
      721 accgtgcgcg agatggagat cctcaggctc atgcaacagg gcgtgtccaa caagactatc
      781 tcccgccacc tgggcatcga gctgtcgacc gtgaagaacc acgttcacag cattctcgcc
      841 aagctcggtg ttcaccggcg cggcgaggcc atctcgctgt tgtatgcaca tgagaaggcg
      901 gcgcgcggtt gggacgtgtt gccgccggtc gaaggctccg actccgccgt cgacgacgac
      961 ccttcattcg aggccgcaat ggcctgacgc ccggcgcggc gtcctacgta ccggcggcgt
     1021 gcccgccgtc ctgtaagcgg ccgccgcctt ttcgaaccga tcgaatatgc cgatcaaact
     1081 accgcacgcc gatgacggcg cgcgcgcgag tcgggtctat tagagaccca tgaactacga
     1141 gatttattgc ggcccgcgga gatgcgttcg tcccctggcg ccaggcgaag ctcctccact
     1201 cggcgcacac ctcgccatgc cttggctcgg ttatacccat cacggcatct atgtcggagg
     1261 cggcaacgtc gtgcactacg gcgcgttgaa gtacaacctg atccggaggc ccgtggaaga
     1321 agtgtcgctg gccgcgttcg ccgagggccg gccggtttac gtggtgatgc attccgaaga
     1381 ggcgttcgag gtcgcggaaa tcatccgtcg cgcgcgttca cggctcggag aggcgcgcta
     1441 ccgcctgttc agcaacaatt gcgagcactt cgtcgaatgg tgcctgcacg accaaccccg
     1501 cagcttcgag gcagaaacag cgctggaatt tccgcgctgg ttcggtgagc gaatcgtggt
     1561 ggccatgctg cgcttcgcgc gctgggcgtt tcgcgctcgc ggggcggtgc cggaatccgc
     1621 acgcgtcgag ccccgtctgc cacgccgtcc atgaattggt gccgggccgg gaaaccggga
     1681 aaacttccac cgaacgcaca acgtcgagtt gtcagacttc aatccggccc ggcaccactg
     1741 tgcgacgccg ccgcggcgcg tgcgcttcga cgcaggatgt cgagcaattg ctgctgtgtg
     1801 gtggtcgggc gccagttgcg gcgcagtgtg aggccgatgg gccgcaccac gtctccggcc
     1861 gggtggggca atgccaccag caaccccgcc tgcatctcgt aatgaatctg gtgcgcggaa
     1921 agcaacatca cgtgatcgct ctcgagcagg aaggcgcgcg ccgccgccat tgaattgcat
     1981 tcgagcggcg ggctgggcaa ctggtgaccc gtcgaggcca gcagggcatc gaaatgaacg
     2041 cgcaatggcg acccggtgcg cggcgctatc cactggaatt gcccgagcgc ggccgcggcc
     2101 acgcgcggac gttgcgcgag cggatgctgg gcgcgcacga cgatggccag cgggtcgtcg
     2161 aacaggtgtt cctggatcac gtccgccgcc ggcgccgggt cgcgcatggc gccaatcagg
     2221 atgtcggatt cgccggcacg caggcccgag agcaggtgct cataggtccc atcgatgatc
     2281 gcgacgccgt gccgcgggtg cttctgcgtg aattcgagca gcgtggccgg cagtagatag
     2341 ctgccggcca gcggcagcgc gctgatgacc gtccgtccgg actctccgcc gctcagcgcg
     2401 tgcacctcgg cgcgcgcctg ttcgatctcg gcgaacgcga gtcgtgcgcg acgcgccagc
     2461 tccgcagctt cgcgggtggg cgtaattccg aagctcgtct tttcgaacag ctgcacgccg
     2521 agcaaccgct cgagctcgcg cgccgagcgg tggatgctgg gctgcgacac gtggcgtgcg
     2581 cgggccgcga tgctgaaatt gccatgttcg acgacggcgc caagcgcttc gagctgccgt
     2641 gagcgcacga gcctctccac gtcgagccgt tccgtgccgg cgttgcgcgt ggccgcgcct
     2701 agcgcctcac gcagctgccc catcgagcgc tccacgcgcg ccaggcagat ttctccggca
     2761 cttgtcaggg agacgcccat gttatgccgc agcaacagcg gtgaaccgaa gtaacgctcc
     2821 aggcccgcga tcgcctgcgt caccgcgggt tgtgaaatgt gcaccgcgcg cgcagcggcg
     2881 ctgacgctgc ccagccgggc gacttcacgg aacgtatgca ggtggcgcag gtttgcgtgc
     2941 atgcgcaatc gtacacctat agcgttttct tatggcgatg gcgtatttct gattggccgt
     3001 ccgacggcgt acgagcggac aatgcacggc gtcgacaacg gggggtggac ggatgtcggt
     3061 cgtggtcact aatgtggagc gcgtcgagcg cggggtcgtc gaggcgcttg cgccctttgg
     3121 cgtcgcaact atccacgagg cgcaggggcc atatggcctc atggcaccgt acatgcgccc
     3181 catctacccg ggcgcgctca tcgccggcac ggcgatcaca gtcagcgtgc caccaggcga
     3241 caactggatg attcacgttg ccgtcgagca atgccggcca ggcgacattc tggtggtggc
     3301 gccgacgtcc gccagcgact gcggttattt cggcgatttg ctcgccagtt cactcaaggc
     3361 ccagggcgtg ctcggcctca tcatcgaagc cggcgtgcgc gacgtgcggg cgttgacaga
     3421 gatgcgtttc ccagtctggt cgcgctatgt ctcggcgaag ggaaccgtga aggccacgct
     3481 gggtagcgtc aacattccca tcatctgcgc cggtgcacag gtgaatcccg gcgatttcgt
     3541 ggtggccgat gatgacggtg tctgcgtcgt gcctcgcgca cgagtcgact cggtgctcgc
     3601 cagtgcgcgc gcgcgcgaac gcaaggaagc ggcagttcgc gcgcgcctcg tgaccgggga
     3661 acttggcctg gacgtctacg aaatgcgcga ggctctcgcc gcgaagggac tcacttaccg
     3721 ggacggcaag gactgacgtt gctcaccgga attccatgca cgctcatgcg cggcggaacg
     3781 tcgaagggac tctatttcaa cgcggccgac ctgccgccgg atcgcgagct gcgcgaccgg
     3841 gtgttgctgg cggccatggg ttcgcccgat gcgcgccaga tcgatggcgc cggcggcgcg
     3901 catccgctca cgagcaaggt cgcggtcatt tcgcgatccg cgcatcccgg cgcggacgtg
     3961 gactatctat tcctgcaggt ggtcgtggac cgggccgagg tcagcgacag ccagaactgc
     4021 ggcaacattc tcgccggcgt cgggccatgg gccatcgaga acgggctcgt gcccgtggcg
     4081 ggacccacga cgacggtgcg cattcacatg gtgaacacgg gaagcctggc agtcgcacac
     4141 gtgcccacgc cacatggagc ggtcgagtac gaaggtgaag cgcgcatcga tggcgtgcct
     4201 ggcaccgccg cacccatcgc attggaattt ctcgatgtgg caggctcgag cagcggctca
     4261 ctgctgccga cgggcgccgt caccgacacc ttcgaaggca tagaggtcac ctgcatcgac
     4321 aacggcatgc cggtgatcat cgttcgagcc gcggatctcg gcgtcaccgg cgccgagtct
     4381 ccggcgcagc tggaggcgaa cgcagcgctg gtggccaggg tcgagcgcat gcggttgagc
     4441 ctgggaccgc ggatgaatct cggcgacgtg acgcgcaaga ccgtcccgaa gatctccctg
     4501 gtgagcgcgc cgagacacgg tggttccatc ggtaccgcca cgttcattcc ccatcgcgtg
     4561 cacgaggcaa tcggggtgct gggcgcggtg agcgtggcca cggcttgcgc aattccgggg
     4621 tcggttgccg cccaggtggc gcaacgcagg gacaacgcgc ggcgcatcga agtggagcat
     4681 ccgagcggat tcttcacggt cgatgtggaa gtcgaacgcg gggattcttt cgtcgttcgc
     4741 ggcgcggcgt tgctgcgcac ggcgcgcaag atcctgcgcg gtgaagtgtt cgtgcccggc
     4801 gctgtctgga gccacgcatg accgcgcctt cggtctgtct gctcggtttt ggagaagtcg
     4861 gccaggtgct gtgcgaggac ctgcgcacgc gtgtcggcca cttgtccgcc tgggacctca
     4921 agttttccga ttcggccagc gagcccaggc gcgctgcggc gcgccatgaa ggcctggcag
     4981 catccgacgc caggcacgcg gtggacggtg cggacctcgt gatcagcgcg gtcacggccg
     5041 cgcagattgt cgaagccgca agttcggccg ccccgcatct gaagcgcaat gcgtatttct
     5101 tcgatctgaa ctcctcgtcg cccgccgcga agcgccaggc ggctggcatc gtcgagtcgg
     5161 ccggcggccg cttcgtcgag gccgccatca tgtcgccgat ttcgccgcgg cgtgcggcgt
     5221 cgcccatgtt gctgggaggt gcgcatgcag acgggttcct gccgctcgct cgcgagttcg
     5281 gattctccgg cgcggaagtg ttttccggga agttaggcgc cgcttcggcg gccaagatgt
     5341 gccgcagcgt cgtcgtgaag ggcctcgagg cgctgatgct cgaatcgctg ctcgcggcgc
     5401 gccagcacgg ggtcgagggc gcggtgctcg aatcgctacg ttcgctgttt ccgcccgaca
     5461 gcagcgcggc aactccgcgc tacatgattt cgcgcgcgct ggtccatggc cgtcggcgcg
     5521 ccgaggagat gggcgaggcc gcaaggacgg ttgccgaggc cggtgtctct tcacgcatga
     5581 gcagtgcctg cgccgagtgg caggaatggg cttcgcatca tcccgacgca gccgccaccc
     5641 cggatatcga cgtgatgctc gacacgctgt tgtccgcgcg cgggagcgcg ccatgctgat
     5701 catcgactgc cacgggcact acaccacggc gccggagccg caccagaagt tccgtgatgc
     5761 gcagctcgtg cgcatcaagg attcgacgct tcctgcgccg gtgccggcgc aggtcagcga
     5821 cgacgagatc cgggagtcga tcgaggccaa ccagcttcgc ctgcagcgtg agcggggcgc
     5881 ggacctcacc ttgttctcgc cgcgcgcttc ggccatgggc catcacgtcg gcgacgagtc
     5941 cgtttccact tcctggacgc gcgcctgcaa cgatctcgtc aaacgcgtcg tcgatctgta
     6001 tccggagaat ttcgccggcg tctgccagct tccgcagtcg cccggcgtgc cgatctcgca
     6061 ttcagtggac gaactcaccc gatgcgtgcg cgatctcggg ttcgtgggct gcaacctgaa
     6121 cccggaccca tcgggcggcc actggaattc gccgccgctc accgaccgtc actggtatcc
     6181 gttctacgag aagatggtcg agctcgatgt gccggccatg gtccatgtct cggcgtcatg
     6241 caatcccaac ttccacgcca ccggcgctca ctacatcaat gccgatacca ccgctttcat
     6301 gcagttcatc cagggtgacc tgttccgcga ctttccgaca ttgcgcttcg tgatcccgca
     6361 cggcggaggg gcggtgccgt accactgggg ccgatatcgc ggccttgcgg acatgctcaa
     6421 gcgcccggcg ctcgacggac acgtgatgcg caatgtgttc ttcgatacct gcgtctatca
     6481 ccagcccggc atcgacctgc tcgtcaaagt tatcgacatc gacaacattc tgttcggttc
     6541 ggaaatgctc ggcgcggtgc gcggcatcga tccgcaaacc gggcatcact tcgatgacac
     6601 acgccgctac atcgatgcgc tggcgatttc cgcggacgcc aggcggcaga tattcgagct
     6661 caacgcgcgc cgcgtctatc cgcggctgga cgtgatgctc aaggcgcgtg gcctatgaac
     6721 gccggtcatt tccaaccgga tccgggttgg ctcagctttc atgcggcgcc aggcaagccc
     6781 acattccgtc caccaccggg cgccgtggac gcgcattgcc acgtcttcgg acctggcgcg
     6841 gtgtttccct acgcgccaga gcgcaagtac acgccggtgg atgcgcccaa ggaaaccttg
     6901 tgggcgctgc gcgatttcct ggggttcgag cgcaacgtga tcgtgcaggg gacctgccac
     6961 ggcaccgata accgcgcact cgtcgacgca cttcgcaact ccgatggccg cgcacggggt
     7021 gtggcaagtg tctcgcgtga tgtcaccgac agcgaactgg ccgacctgca cgacgccggc
     7081 gtgcgtggcg tgcgcttcaa cttcgtgaag cgcctcgtcg atgtgacgcc gcccgccgtg
     7141 ctgcacgaaa tcgcgacccg catcgcgccg ctgggttggc acgtggtggt gtatttcgaa
     7201 gcccacgacc tgcccgagct cgcgcccttc atcgcctcgc tgcccaccat cgtggtcgtc
     7261 gatcacatgg gccggcccga tgtacggctg ccggtggagg gactggagtt cgagcgtttc
     7321 gtccgcttca tgcgcgagca ctccaacgtc tggtcgaagg tgagtggcgc ggagcggctg
     7381 tcgctgaagg ggccgccggg ttattccgac ttcgtgccgt tcgcgcggcg aattgttgaa
     7441 tcctttcccg gacgtgttct ctggggcacg gactggccgc acccgaacat gaagtcgcac
     7501 atgccggacg acggcgatct cgtgaacctg ctgccacgaa ttgcgcccac accagaactg
     7561 cagcgctttc tgctcgtcga caacccaaag agactctact ggtgaaaact tatggcgaca
     7621 ttcccggcac gacggtgttc gacgcggagc aatcgcgcaa gggttatcac ctgaacatgt
     7681 tctgcatgtc cttgatgaag gcggccaacc gcgagcgctt caagtccggc gagcgcgcct
     7741 atctggatga atggccaatg accgacgagc agaagcaggc cgtgctcgat cgggactaca
     7801 accgcatgat cgcgctcggc ggcaacatct atttcctcgc gaagattttc gccaccgacg
     7861 gccgcagttt tcagtatgcg gcggcgctca tgaccggcat gagccaggaa gattacgcac
     7921 gcatgatgct cgatggcggc cgggcctatg tgcccggaaa gccgaaggag ggcgtgtaat
     7981 ggcgcgcatc tccgccggca tcacctgctc gcacgtgccc gcaatcggcg ctgccatcga
     8041 tctgggcaaa acgaaggagc cgtattgggc gccgctgttc gctggcttcg aattctcgaa
     8101 gcagtggatg gccgcgaata cgcccgacgt ggtgttcctc gtctacaacg atcacgccac
     8161 cgcattcagc ctcgagatca tcccgacgtt tgcaatcggc tgcgcgccgg aattcgcccc
     8221 cgcggacgag ggctggggcg cgcggccagt gcccgtggtt cgcggcgctc ccgagctggc
     8281 cgcgcacatc gccgcgagcg tcatcgaatc ggacttcgat ctcacgctcg tcaacaagat
     8341 ggatgtggat cacggcctca cggttccgct gtcgctgatg ttcggtcagc cgcagcgctg
     8401 gccctgcctc gtcattccgt tcgcggtgaa cgtcgtgcag tacccgccac cctcgggtaa
     8461 tcgttgttat cagatcggca aggcgatccg ccgcgccatc gacagcttcg accaggatct
     8521 caatgtgcag gtctggggca ccggcggcat gagtcaccag ctccagggtc cgcgcgcggg
     8581 cctcatcaac aaggagttcg acgcgaaatt cctggacggc atgatcaagg atcccgaggc
     8641 gcttgcgcgc attcctcgcg aacaatactt gcgtgaggcc ggttccgaag gcatcgagct
     8701 cgtgatgtgg ctcatcatgc gcggcgctct cgaaggcccc gtgcgcgaag tccatcgctt
     8761 ctatcacgtg cccgcgtcga atacggccgt cgggcatctc atcgtggagc cccagtcatg
     8821 agcaagtcca tgacccagcc cctgaagctg gtcgtcgccg gtgtcggggc ttttggccag
     8881 aagcatctgg atgcctgcaa attgatcgag ggcgtcgagg tcgaatccgt ggtgggacgc
     8941 cagctcgcgc ccacgcaaga ggtagcgcgc aaatacggcg ttgcgcatgc gaccacggat
     9001 ctcgccgagg cactggcccg tccgggagtc gacgccgtca ttctcgccac tccgacacag
     9061 atgcacgcgg cgcaaagcgt gcagtgcctg aacgcgggca aacacgtgca ggtggaaatc
     9121 ccgctcgcgg attcgctgaa g
//
LOCUS       DASQJS010000455        11147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1240554, whole genome shotgun sequence.
ACCESSION   DASQJS010000455 DASQJS010000000
VERSION     DASQJS010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11147
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240554"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..964
                     /locus_tag="VEW08_18005"
     CDS             158..964
                     /locus_tag="VEW08_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009390813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HYJ42691.1"
                     /translation="MHMGFLSGKRALIVGVASDRSIAWGIAQAMHREGAELAFSYVNE
                     KFKERVQPLAESLGSKLTMQLDVTNDAEIDSAFATLRQSWDSLDIVVHAVAYAPREAL
                     AGGFVESTSREAFRVAHDVSSYSLTALARAAKPLMAGRAGAILTLSYLGAVKSIPSYN
                     VMGLAKASLEANVRFLAADMGPHNVRVNGISAGPIKTLAAAGIAGFRKMLSRVADVAP
                     MRRNVTLEDVGNAAAFLCSDLANGITGEILYVDGGFSTVGMSFPQEAGEG"
     gene            complement(991..2313)
                     /locus_tag="VEW08_18010"
     CDS             complement(991..2313)
                     /locus_tag="VEW08_18010"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HYJ42692.1"
                     /translation="MGSAGAETKPTPVLPILIAMVLGGAVGYAAWPDGMIGGVHLLPL
                     FDTLGTMFINLLKMLIVPLILASVITGVASLGSGPDLGKLGGKTLGFYVITTLIAVLI
                     ALALVNLVVPGVIDGEPQRHALALTRDAGEVAAGVKSRAETSVLDSIKGIVPSNMVDA
                     ALNTKMIGLVLFSILFGFFLARIEQPYRENVYGFWRGVFLIMMRMTGFVMFLAPIGVF
                     ALIAKVVAMSGFAAGHSLALFVICVLGGLLLYGFVALPILLATVARVNPWRIYPAMAP
                     ALLTAFSTASSSATLPLSLDCLEQRAKVSPRIAGFVMPLGASMNHAGSALYECAAAMF
                     IAQAYGLHLTFAAQFTVVILALITSMGMAGIPAASLVGIAVILAAVGLPAEAIGVLLV
                     FDRILDMCRTSINVLADAACTVIVARLEGETGILPAPSKTEMGSGTVS"
     gene            complement(2320..4224)
                     /locus_tag="VEW08_18015"
     CDS             complement(2320..4224)
                     /locus_tag="VEW08_18015"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HYJ42693.1"
                     /translation="MLQRIRDGLQGQKWLAWIILGLIGATFVFWGGSSSLDFTGVSGT
                     TAAEVDGVEIPATEATRAWSDMQARWSRQFGTDIPPEQRVAMQQNILDGLVLRKLVEM
                     RLNDSNYRVSDAAVLTEFQKIPEFQGPDGNFDVGTARAVLAQINKSEAEYFADTRSQL
                     LINQLQQGIGGSYFLTRAEQQRLFNLENEEREVQYATFEADKFVGSDPIDDAAIKAYY
                     DKNGDRFMTTESVALEYAELRLEQLATQIAPTDADLQKLYEDNRAMYVLDERRRARHI
                     VIAVNGDDDAGALKQAESVAAEARSGKDFAELAKKYSSDSTASEGGDLGFVQKKDFPG
                     PLGDTLFTMNIGDVSAPVKSQFGYHILKLEQVEAGEAKPFADVRAELDSQYRQDRSAD
                     LFGERQEELSKRLDKGMTDIDKLAQDLGLTRGSVPEFLRGGGAEPLGSSVDLQQTVFS
                     DATLNQGKIGGPVGLGDDRLVVVKVTAHHKAAVKPLDVVRDEIVGLLKQERGEAAAKA
                     AAEAMVARLTAGEQLDALAKASNFSAEPARFMSRGDPSIPAALRTAIFESPRPTDKPV
                     IQSASLDDGSTAVYVVTRTRVADSTTNPTLVQQQNAQLQQRSAQGEVAAYINEAKRKA
                     KIVKNMSVFE"
     gene            complement(4255..4331)
                     /locus_tag="VEW08_18020"
     tRNA            complement(4255..4331)
                     /locus_tag="VEW08_18020"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4295..4297),aa:Asp,seq:gtc)
     gene            complement(4479..5573)
                     /locus_tag="VEW08_18025"
     CDS             complement(4479..5573)
                     /locus_tag="VEW08_18025"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HYJ42694.1"
                     /translation="MAPTTIVTRTLAFMTGIGATLLLLEGALRLLPVLDGTFAADPRP
                     SWPVHTMIPNSQFTYSTGWNLQNIHRGRINDYGYVAPFDYSPAIAGRTGAGREGTVAV
                     FGDSYVEAQMNDYGDTLQGALNDYLKSRRTVLNFGTAGAEMPDYLGIAPLVRRDFSPQ
                     WAVFVITLGDFTRGFTAGPGFFKWQPDRSPPVKLVPEIHRSDLSKWMRTLALVRYLRG
                     NLSMRPDELIRLRRGADAAAAAGPCRDEVLSKQDEVLIESFVRELPMALGLPPDRVIL
                     VFDSDRKAIYAGKTRAEAQKCQARPARANDHLQQLAARADLRVIDSYPVFKQYFDERQ
                     GPLDRSPVDAHWNAAAHRLMAREVARIIER"
     gene            complement(5579..7078)
                     /locus_tag="VEW08_18030"
     CDS             complement(5579..7078)
                     /locus_tag="VEW08_18030"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HYJ42695.1"
                     /translation="MLFTSGEFLFVFLPITLLVFYAAARFAGRAAAAAWLAVASLIFY
                     GYWRIEHTVLLVLSIGFNYYFGEWILRARHGGKPSSRVLLTIAVAANLGALAYFKYAD
                     FFLRTAAELSGADIPLLGVVLPLGISFFTFTQIAYLVDVHAGKVVERNPIHYALFVTY
                     FPHLIAGPVLHHAEMMPQFRSADIYRPALRNFVIGMAFLLMGLAKKVLIADSVAPVAN
                     MVFGAGADLPLATSAAWLGVTAYTLQIYFDFSGYSDMAVGLSLLIGVRLPYNFNSPYQ
                     AWNITEFWRRWHMTLSRFLRDYLYVPLGGNRHGRGRRYVNLMLTMLLGGLWHGASWTF
                     VIWGGLHGAYLVVNHGWQWLREKMGWQRASTGLGAILGRGASVALTLLCVMVGWVFFR
                     AVDTASAQNILTSMFGLRDGGLPITSLAGYSQDAVVWIALFALLALFGRNSQALIDGH
                     LTAWLDRRTQRKHHGEWLAWMTGAMCVGVAWMAIAAASRTVTEFIYFNF"
     gene            complement(7208..7282)
                     /locus_tag="VEW08_18035"
     tRNA            complement(7208..7282)
                     /locus_tag="VEW08_18035"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7248..7250),aa:Val,seq:tac)
     gene            complement(7480..9858)
                     /gene="lon"
                     /locus_tag="VEW08_18040"
                     /pseudo
     CDS             complement(7480..9858)
                     /gene="lon"
                     /locus_tag="VEW08_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
     gene            complement(10050..>11147)
                     /gene="clpX"
                     /locus_tag="VEW08_18045"
     CDS             complement(10050..>11147)
                     /gene="clpX"
                     /locus_tag="VEW08_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HYJ42696.1"
                     /translation="EYVIGQERAKKTLSVAVYNHYKRLQTRGGTAGRGKDDVEISKSN
                     ILTIGPTGCGKTLLAETLARLLNVPFTIADATTLTEAGYVGEDVENIIQKLLQKCDYD
                     VEKAQTGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTIASVPPQGGRKH
                     PQQEFLQVDTSNILFICGGAFAGLEKIVMNRTQKGGIGFTSEVRDVNTRPHGTDIFKE
                     VEPEDLIKYGLIPEFVGRLPVVATLEELDEKALISILTEPKNALTKQYRRLFDMEGAE
                     LEFRDEALSAVAKKAMQRKTGARGLRTILENVLLDTMYELPGLKNVSKVVVDDTVIEG
                     HQKPYIVYKSETATTPDDAATPRRVSGAGLS"
BASE COUNT         2036 a         3488 c         3529 g         2094 t
ORIGIN      
        1 tctccgacct ggtcaactgg aacggcatca gcaagcactc gatgctgcgt ccgggccaga
       61 aactcacggt caacgttcgc cgccactgat ttcgtccccg gcggagggcg aggctggcag
      121 aatgcggcct cgctaactca aaggccagat aaaggggatt cacatgggct tcctgagcgg
      181 aaaacgcgcg ctcatcgtcg gcgtcgcgtc agatcgttcc atcgcgtggg gaattgccca
      241 ggcaatgcat cgcgaaggcg ccgagctcgc gttctcgtac gtcaacgaga agttcaagga
      301 acgcgtacaa ccgctcgccg agtcgctggg ctcgaaactc accatgcagc tcgacgtcac
      361 caatgacgcc gagatcgact ccgcgttcgc cacgctccgg cagtcctggg attccctcga
      421 catcgtcgta cacgcagtcg catacgcgcc gcgtgaagcg ctggccggcg gtttcgtcga
      481 gagcacgtcg cgggaggcgt tcagggtcgc gcacgatgta tcgagctaca gcctcactgc
      541 cctggcccgc gccgccaagc cgctcatggc gggacgcgct ggcgccatcc tcacgctgtc
      601 ctatctcggc gcggtgaaat ccatccccag ctacaacgtc atggggctcg ccaaggccag
      661 cctcgaggcg aacgtgcggt tcctggccgc cgacatgggg cctcacaatg ttcgcgtgaa
      721 cggcatctcc gccgggccca tcaagacgct cgcggccgct ggcatcgccg gcttccgcaa
      781 gatgctgagc cgcgtcgccg acgtcgcgcc gatgcgccgc aacgtcacgc tcgaggacgt
      841 gggcaatgcc gccgcgttcc tgtgctcgga cctcgccaat ggcatcaccg gcgaaatcct
      901 ctacgtagac ggcgggttca gcacggtggg catgagcttc ccgcaggaag ccggcgaagg
      961 ctgactagaa acggggtcag gcaccgtttc ctaggaaacg gtgcctgacc ccatttccgt
     1021 ctttgacggc gccgggagga ttccggtctc gccttccagg cgcgccacga tcaccgtgca
     1081 cgctgcgtcg gccagcacgt tgatcgacgt gcggcacatg tcgagtatgc gatcgaatac
     1141 cagcagtacg cctatggcct cggcgggcaa tcctaccgcc gccaggatca ccgcaattcc
     1201 cacgagtgat gcggccggaa ttccggccat acccatggac gtgatcaacg cgaggatgac
     1261 cacggtgaac tgcgcggcga acgtcaggtg cagtccgtac gcctgcgcaa tgaacatggc
     1321 ggcggcacat tcgtacaacg cgctgccggc gtgattcatc gaggcgccga gcggcatgac
     1381 gaatccggcg atgcgtggcg acaccttcgc gcgctgctcg aggcagtcga gcgacagcgg
     1441 cagcgtcgcg gatgaagacg cggtcgaaaa cgcggtgagc agtgcgggcg ccatcgccgg
     1501 atagatgcgc cacggattca cgcgtgccac ggtggcaagc aggatcggga gcgcgacgaa
     1561 gccgtacaac aagagcccac cgagcacgca aatgacgaac agtgccaggg aatggcctgc
     1621 tgcgaagccc gacatcgcca ccactttggc gatgagcgcg aagacgccga tgggcgccag
     1681 gaacatcacg aatcccgtca tgcgcatcat gatcaggaac acgccgcgcc agaagccgta
     1741 cacgttctcg cgatacggct gctcgatacg cgccaggaag aacccgaaca gaatggagaa
     1801 caacaccagc ccaatcatct tggtgttcag cgcggcgtcg accatgttcg aaggcacgat
     1861 gcccttgatc gaatcgagga cgctggtctc ggcgcgagat ttgacgcccg ccgccacctc
     1921 acccgcgtcc ctggtcagcg cgagcgcatg acgctgcggt tcaccgtcga tgacaccggg
     1981 cacgacgagg ttcaccagag ccaatgcaat caacaccgcg atgagcgtgg tgatgacgta
     2041 gaagccgaga gtcttgcctc cgagcttccc gagatcgggg cccgaaccga gcgaggccac
     2101 gccggtgatc accgacgcaa ggatcagcgg cacgatgagc atcttcagca ggttgatgaa
     2161 catcgtgccg agggtgtcga acagcggcaa cagatgaacg ccaccgatca tgccatcggg
     2221 ccacgcggca tagccgacgg caccgccgag caccatggcg atgagaatgg ggaggactgg
     2281 agtgggcttg gtttctgctc cggccgatcc catcaggcgt tactcgaaga cgctcatatt
     2341 cttgacgatc ttcgccttgc gtttggcttc gttgatgtac gcagccactt cgccctgcgc
     2401 cgagcgctgc tggagctgcg cattctgctg ctggacgagc gtcggattgg tggtggagtc
     2461 cgccacgcgc gtgcgcgtta cgacgtacac cgcggtcgaa ccgtcgtcga gtgacgcgga
     2521 ctggatgact ggcttgtccg tcgggcgtgg cgattcgaag atcgccgtgc gcagcgccgc
     2581 gggaatcgac ggatcacccc ggctcatgaa gcgcgcgggc tcggcgctga aattcgaagc
     2641 tttcgccagc gcgtcgagct gctcgccggc agtgagcctg gcaaccatgg cttcggccgc
     2701 tgccttcgcg gccgcctcgc cgcgttcctg cttcagcagg ccgacgattt cgtcgcgaac
     2761 cacgtccagc ggcttcaccg cggccttgtg atgcgccgtg actttgacga ccacgagccg
     2821 gtcgtcaccc agtccgacgg ggccgccgat ctttccctgg ttgagcgtcg catcgctgaa
     2881 tacggtctgc tgcaggtcca cgctcgagcc cagaggctcg gcgccgccgc cgcgcaggaa
     2941 ttcgggcacg gaaccacgcg tgaggccgag gtcctgcgcg agcttgtcga tgtccgtcat
     3001 gcccttgtcg agacgcttgg acaattcttc ctggcgttcg ccgaacaggt ccgccgagcg
     3061 gtcctggcga tactgggaat cgagctcggc acgcacatcc gcaaagggct tggcttcacc
     3121 tgcctcgacc tgctcgagct tgaggatgtg ataaccgaac tgcgatttga ccggcgcgga
     3181 tacgtcgccg atgttcatgg tgaacagtgt gtcgccgagc ggaccgggga agtccttctt
     3241 ctgcacgaag cccaggtcac cgccctcgct cgccgtcgaa tcgctcgaat attttttcgc
     3301 gagttcggcg aagtccttgc cggagcgcgc ctcggcggcc acgctttcgg cctgctttaa
     3361 cgcgccggcg tcgtcgtcac cattcaccgc gatgacgatg tggcgcgcac ggcggcgctc
     3421 gtcgagcacg tacatggcgc gattgtcttc atagagcttc tgcagatcgg cgtccgtcgg
     3481 cgcgatctgc gtggccagct gttcgaggcg cagctcggcg tactcgagcg caacagactc
     3541 cgtggtcatg aagcgatcgc cgttcttgtc gtagtaggcc ttgatggcgg catcgtcgat
     3601 cggatcgcta ccgacaaact tgtccgcctc gaacgtcgcg tactgcactt cgcgctcttc
     3661 gttttcgagg ttgaacagac gctgctgttc ggcgcgcgtg aggaagtagg aaccgccgat
     3721 gccctgctgt agctggttga tcagcagctg cgaacgggtg tccgcgaagt actcggcctc
     3781 ggacttgttg atctgtgcca ggaccgcgcg tgccgtgcca acatcgaagt tgccatccgg
     3841 gccctgaaat tcggggatct tctggaattc ggtcaagacg gcagcatcgc tgacccgata
     3901 gttcgaatcg ttgagccgca tctcgacgag tttgcgcagg accaggccat cgaggatgtt
     3961 ctgctgcatc gccacccgct gctcgggcgg gatgtcagtg ccgaactgtc gcgaccagcg
     4021 cgcctgcata tcgctccagg cgcgagttgc ttcggtggcg gggatttcga cgccgtcgac
     4081 ttcggcggcc gtggtgccgg aaacgccggt gaagtccagg gagctcgaac caccccagaa
     4141 aacgaacgtg gccccaatga ggccgagaat gatccatgcg agccatttct ggccctgcag
     4201 cccatcacga attcgttgca gcatcccctg tcctgaaaac gagtctgaaa agattggcgg
     4261 agcggacggg actcgaaccc gcgacccccg gcgtgacagg ccggtattct aaccaactga
     4321 actaccgctc caattttgtg ggacctgagg gtgtcggccc tcgcccctcg ccttgtactg
     4381 gcgatccctt gcgacgcgct gaaactgacc caggccgtcg gaagggcgcg aagtttagca
     4441 gagaatcgga aaagtacagt tgagatgcat gcccgaatct agcgttcgat gatgcgtgcc
     4501 acttcccggg ccatgagccg atgcgcggcg gcattccagt gggcgtccac cggggaccga
     4561 tccagtgggc cctggcgctc gtcaaagtac tgcttgaaga caggatagct gtcgatcaca
     4621 cgcaggtctg cccgcgccgc aagctgttgc agatggtcat tggcccgagc aggccgtgcc
     4681 tggcatttct gcgcctcggc ccgggttttc ccggcataga tcgccttgcg gtcggagtcg
     4741 aaaaccagga tgacgcggtc gggcggcagc ccgagcgcca tcggcaattc gcgcacgaag
     4801 gactcgatca gcacttcgtc ctgctttgac agaacctcgt cgcgacacgg gccggccgcc
     4861 gccgccgcgt cggcgccacg ccgcagccgg atgagctcgt cgggccgcat ggagaggttg
     4921 ccgcgcagat agcgaaccag cgcgagcgtg cgcatccatt tgctcaagtc cgagcgatgg
     4981 atttccggaa ccagcttcac gggcggagag cggtcgggct gccatttgaa aaatcccggg
     5041 ccggcggtga aaccgcgtgt gaaatcgccc agcgtgatca cgaacaccgc ccattgcggt
     5101 gagaaatccc ggcgcaccag cggcgcaatg cctagatagt ccggcatctc ggcgccggca
     5161 gtgccgaagt tgagcacggt ccgccgcgac ttcaggtagt cgttgagcgc gccttgcaga
     5221 gtgtcgccgt agtcattcat ctgggcttcg acgtagctgt cgccgaacac ggcgaccgtg
     5281 ccttcgcgcc cggcgccggt gcgcccggcg atcgccggag aatagtcgaa cggcgccacg
     5341 tagccgtagt cgttgatgcg cccgcgatgg atgttctgca ggttccagcc cgtggaatac
     5401 gtgaactgcg aattcgggat catcgtatgt accggccagg agggtcgcgg atccgctgca
     5461 aacgtaccgt ccagcaccgg caacaaccgc aacgcccctt cgagcagcag cagcgtggcg
     5521 ccgatgccgg tcatgaaggc cagcgttcgc gtgacaatgg tggtcggtgc catggtagtt
     5581 agaagttgaa gtagatgaat tccgtgaccg tgcgcgaggc cgccgcgatc gccatccacg
     5641 cgacgccgac gcacatggcg cccgtcatcc aggccagcca ttcgccatgg tgtttgcgct
     5701 gcgtccggcg atcgagccag gcggtcaggt gtccgtcgat caacgcctgc gagttgcggc
     5761 cgaacagggc gagcagtgca aacaacgcga tccagaccac cgcatcctgc gaatagccgg
     5821 ccagcgaggt gatgggcagg ccaccgtcgc ggaggccgaa catgctggtg agaatgttct
     5881 gcgcggaggc cgtgtccacg gcgcggaaaa atacccagcc gaccatgacg cacaacaacg
     5941 tgagcgccac gctcgcgcca cgcccgagga tcgcccccag gccggtggag gcgcgctgcc
     6001 agcccatttt ctcgcgcagc cactgccacc cgtgattgac gaccagatag gcgccgtgca
     6061 ggccacccca gatcacgaag gtccaggaag caccgtgcca gaggccgccg aggagcatcg
     6121 tcaacatcag gttcacatag cgacgcccgc ggccgtggcg attcccgccg agcggcacgt
     6181 agaggtagtc acgaagaaat cgcgacagcg tcatgtgcca tcgacgccag aactcggtga
     6241 tgttccaggc ctggtacggc gagttgaagt tgtacggcag cctcacgcca atgagcaggg
     6301 aaaggcccac cgccatgtcc gaatatcccg agaaatcgaa atagatctgc agcgtgtagg
     6361 cggtcacacc cagccaggcg gcggacgtgg cgagcgggag gtccgcgccc gcgccgaaca
     6421 ccatgtttgc caccggcgcg acgctgtcgg cgatcaggac tttcttcgcg aggcccatga
     6481 gcaggaacgc catgccgatg acgaagtttc gcaaggccgg gcgataaata tccgcgctgc
     6541 ggaactgcgg catcatctcc gcgtgatgca ggacgggtcc ggcgatcagg tgcggaaagt
     6601 aggttacgaa caacgcatag tggatcggat tgcgttccac caccttgccc gcgtgcacgt
     6661 ccacgagata cgcaatctgc gtgaatgtga agaacgaaat gcccaggggc agcaccacgc
     6721 ccagcagcgg gatatccgca cccgacagtt ccgcggccgt cctcaggaag aagtccgcgt
     6781 atttgaagta ggccagcgcg cccagattgg cggcgacggc gatggtcagg agcacgcgcg
     6841 aggacggctt gccaccgtgg cgggccctca ggatccactc accgaagtag tagttgaacc
     6901 cgatggagag caccagcagc accgtgtgtt cgatgcgcca gtagccgtag aagatcagcg
     6961 atgccaccgc cagccaggcg gcagccgcag cccggcctgc aaaacgcgca gccgcgtaga
     7021 acaccagcag cgtgatcggc agaaatacga agaggaattc gccgctggtg aacagcaaac
     7081 gtggggcccg cgcaactagc tactgggccg ccactatgac gctcggcgac tgacgagaaa
     7141 tctcaaattg gtgtaaggcc cgtcctaccg gccttatcaa ttccccgacg gccgccccgt
     7201 tcggggttgg tgggtgctga gggtttcgaa cccccgaccc tcgccgtgta aaggcgacgc
     7261 tctaccgctg agctaagcac ccgtcattcc cgggggcgga agatacgccg cccatgccgg
     7321 cctcgacaaa taaaacgggc tccccacctt cgcggggagc ccgtcaagcc gactatctca
     7381 atgcgcctgg accagtttcg tagaacgctt gccggcgcga cgcgggcgtt tttcgggtac
     7441 tgccggaggc gtcgggagtc gcggcacagg ccggctggcc agcgcgatct cgaacacttc
     7501 gtcgatccat ttcaccgggc ggatatcgag cttgcccttg atgttgtcag ggatctcggt
     7561 gaggtccttc tcgttgtcga cgggaatcat gacggtggtg atgccgccgc gatgcgctgc
     7621 aagcagcttc tctttcaatc cgccgatagg caggacttcg ccacgcaggg tgatttcacc
     7681 cgtcatggcc acatccgagc gcaccggaat cttggtgagc gccgagacca gtgccgtgca
     7741 catgccgata ccggcagacg gaccgtcttt cggcgtcgca ccttcgggca tgtgcaggtg
     7801 cacgtcgtgc ttctgataga agtccgggtc gatgccgagc acgttggcgc ggctgcgcac
     7861 caccgtcatt gcggcctgga tcgactcctg catgacttcg ccgagctgac cggtgtgctg
     7921 cagcttgccc ttgccaggca ccactgcggc ttcgatggtc agcaattcgc cgccgacttc
     7981 cgtccaggcg agacccgtga cgtggccgat gcggttttcc tcttcggcct tgccgtaacg
     8041 gaaacggcgc acgccgagga acttgtcgag gttcttggaa ttgaccgcca cctgcttgtc
     8101 ggccgccttc tcggctttct ccgatttttc acctttcttc ggctcggctt tcttcgccga
     8161 catcagcagc tgtttgacag ctttgcggca gatgcgcgcc agctcgcgct ccagatttcg
     8221 cacgccggct tcgcgcgtgt agtagcgaat gatgtcgagg atgacgtcgt catggacctt
     8281 gagctcttcg ggcgcaagac cgttctgttt cacctgcttg ggcaacaggt atttcttcgc
     8341 gatattgagt ttttcgtctt ccgtgtaacc gggcaggcga atgacttcca tgcgatcgag
     8401 cagcggcgcc ggaatgttca acgtgttcgc cgtgcagacg aacatcacct gcgacaggtc
     8461 gaggtcgact tcgaggtagt ggtcggcgaa tgtcgagttc tgctcggggt cgagcacttc
     8521 cagcagcgcc gaggacgggt cgccacggaa gtccatcgac atcttgtcga tctcgtcgag
     8581 caggaacagc ggattgtgca cggcggtctt cgccatgttc tgcacgatct tgccgggcat
     8641 ggagccgatg tacgtgcggc gatggccgcg gatctcggcc tcgtcacgca cgccacccag
     8701 cgacatgcgc acgaacttgc ggttcgtggc gcgcgcaatg ctctggccca acgaagtctt
     8761 gcccacgccg ggcggaccga cgagacacag gattggaccc ttgatcttgt cgacgcgctg
     8821 ctggacggcc aggtactcga cgatgcgttc cttgaccttc tccagcccga agtgatcttc
     8881 ctcgagcacc ttttcggctt tcgcgatgtc cttgaggatc ttggtgcgtt tcttccacgg
     8941 caccttcacc agccagtcga tgtagttgcg caccacggtg gcttccgccg acatgggcga
     9001 catcatcttg agcttgttga gctcggcatt ggccttctcg agcgcctctt tcggcatgcc
     9061 cgctttcttg atgcggttct cgagatcgga gagttcgttg ccgccctctt ccatttcgcc
     9121 gagctctttc tgaatggcct tcatctgctc attcaggtag tactcgcgct ggctcttctc
     9181 catctgcgcc ttgacgcggc cgcggatgcg cttctcgatc tgcagcacgt ccatctcgcc
     9241 ttcgatggcg acgagcacgt gttcgaggcg cttgcgcacg tcgaggattt ccagaacctt
     9301 ctgcttctcg ggtagtttga gcgccatgtg cgcggcaacc gtgtccgcga gacggccggg
     9361 ctgctcgatg ccggcgagcg cggtgagcac ctcgggcggc actttcttgt tgagcttcac
     9421 gtactgctcg aactgcgaga tcaccgaacg ggcgagcacg tcgagctctt tctcgtcgta
     9481 ggtgtcggcg tcgggttcgg tggtgacgtc ggaggtgtaa tgctcgccgg tgtgcatcgc
     9541 ttcgatgcgc gcgcgatcga cgccttcgac caggaccttg accgtgccgt cgggtagttt
     9601 gagcagctgc aggatcgagg cgaccgtgcc gatgcggtag aggtcatcgg tcttcggatc
     9661 gtcgacgtct ggctgcttct gcgcgatcag catgatgcgc ttgtcggcct tcatggcgac
     9721 gtcgagcgcg tgaatggact tttcgcgtcc gacgaacagc gggatgacca tgtgcggata
     9781 gaccaccaca tcgcgtagtg gcagaaccgg caggccctgg atggagacag acttggaagt
     9841 cgaggcggat tcggtcacgc gcggggccct caacgcacca gcgccgccgc aaaatgcagc
     9901 gcgccgtggc tgtatttagt caaagtgtaa cgcgggagat ggggctacgc cgggacgttt
     9961 caacgcctgc gacgcagttc ccgattcgac ggtaggtgta aaggcagaaa cttgccggtg
    10021 gggaagcacc ggtaccgtag gcaattcgcc taactcaagc cagcgcccga cacgcgacgc
    10081 ggcgtcgcgg catcgtcggg cgtggtcgca gtctcggact tgtagacgat gtagggcttt
    10141 tgatgacctt cgatgaccgt gtcgtcgacc acgaccttcg aaacgttctt caggccggga
    10201 agctcgtaca tcgtgtcgag cagcacgttt tcgagaatgg tgcgcaggcc gcgcgcgccg
    10261 gtcttgcgct gcatggcttt cttcgcgacc gcgctcaggg cctcgtcgcg gaattcgagc
    10321 tcggcgcctt ccatgtcgaa cagacggcgg tactgcttcg tgagcgcgtt cttgggctcg
    10381 gtgagaatgg agatgagcgc cttttcgtcg agctcttcga gcgtcgccac gacgggcaga
    10441 cggccgacga actcgggaat gaggccgtac ttgatgagat cttccggctc tacttccttg
    10501 aagatgtcgg tgccatgcgg acgcgtattc acgtcccgca cttcggaggt gaaaccgatg
    10561 ccacccttct gggtgcggtt catcacgatc ttctcgaggc cggcgaatgc gccaccgcag
    10621 atgaagagga tgttcgaggt gtcgacctgc aggaattcct gctgcgggtg cttgcggccg
    10681 ccctgcggcg gcaccgaggc aatggtgcct tcgatgagct tgagcagcgc ctgctgcacg
    10741 ccctcgcccg acacgtcacg ggtgatggac gggttgtcgg acttgcggga aatcttgtcg
    10801 atttcgtcga tgtaaacgat gccggtctgc gccttctcga cgtcgtagtc gcacttctga
    10861 aggagcttct ggatgatgtt ctcgacgtcc tcgccgacat agccggcttc cgtcagcgtc
    10921 gtggcatcag caatcgtgaa cggcacgttg agcagtcgcg cgagcgtctc ggccagcagc
    10981 gtcttgccgc agccggtggg accgatggtc aggatgttgc tcttggagat ctcgacgtcg
    11041 tccttgccac ggccggcggt gccgccgcgc gtttgcagac gcttgtagtg gttgtacacc
    11101 gcgaccgaga gcgtcttctt cgcccgctcc tggccgatga cgtattc
//
LOCUS       DASQJS010000456         6773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2815042, whole genome shotgun sequence.
ACCESSION   DASQJS010000456 DASQJS010000000
VERSION     DASQJS010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2815042"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VEW08_18050"
     CDS             complement(<1..894)
                     /locus_tag="VEW08_18050"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HYJ42697.1"
                     /translation="MAPIPIVNPTIGNGLAVAALMLYKIDPKSPVSTSAVAAGYTDTD
                     SWGVAIMQDAKFAEDRWRILGGLAIALAQYELYVPDISPDFHFSTEQRLAGGLVQVMR
                     RVAPELYAGLRYMRATVNFPRPEETQGLIPEEGIDFNIGGLGVAAEWDSRNHSFQPNG
                     GYRAVFRTNFSRDTFGADLDYDTYSLAFNHYRKGFRDQDVFAARVSLCATSDDTPFFE
                     RCQFGSSNDLRGYPVGRYYDDAMYAAQVEYRAPLWWRLGAVAFVGVGSVAGSFEDLGS
                     SDTLAAGGVGLRILASKEQRLN"
     gene            complement(1063..1674)
                     /locus_tag="VEW08_18055"
     CDS             complement(1063..1674)
                     /locus_tag="VEW08_18055"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HYJ42698.1"
                     /translation="MSLLSAGANAQSRGEGWEFGADLLYLNSNDVDFDGGTTVAFDSD
                     IGFSVYGGYRFSDKLEVQFGLDWSNVDYDASLQLDPAGSVGVSGEIEAFTPFIKGSFN
                     FVDGPITPYVTAGIGWAFIDTNIPNGRPVTGCWWDPWYGYICTTVQDTRTSDSFTYNA
                     GLGVRWDLSTGYSLRLSYEKQWYDLENASPDFDQFRLGVVMRY"
     gene            complement(1982..2557)
                     /locus_tag="VEW08_18060"
     CDS             complement(1982..2557)
                     /locus_tag="VEW08_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42699.1"
                     /translation="MRRSIFLSIIGLTAVLGGVSAPALAADCSTAQFSEAVLARFPNI
                     RLVCSEIIEKDGQQYAVVKGDLVRTGTNAVYMKFNLADGTKSDTRKIETRPEFRVKVD
                     GKPVSVRNLAVGQPLTAYVKVSDPVISLEPHEPTEPLASYPIEEAAPAMAASTAEMPA
                     TASPMPTVAGLGALLLAFAGMLTFFRLRRQS"
     gene            complement(2574..3221)
                     /locus_tag="VEW08_18065"
     CDS             complement(2574..3221)
                     /locus_tag="VEW08_18065"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HYJ42700.1"
                     /translation="MSHSTHGPSNGTRGLARVLRWSEAAAYLAGALLCSLFALAQADS
                     ALGSTNAIEEFAASLNPPDQTLWAAGRVRDYQAAQQSPMKAVAVLTIPTLKLEVPVFE
                     HDDELSLNRGAGLVAGMGTPDTGGNVGIAGHRDGFFRVLKDIKVGDLIEVRTKIKMHR
                     YRVTSIDIVEKTDKRLLADTDMPGVTLVTCYPFYYLGNAPQRFIVAATYEWPSES"
     gene            3336..5423
                     /locus_tag="VEW08_18070"
     CDS             3336..5423
                     /locus_tag="VEW08_18070"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HYJ42701.1"
                     /translation="MAAHTIHSTAHNQSRDRLLGNPATDHALGALALAAGYYATAQIG
                     EALAFPNAPVSALWLPNALVMGALLLAPMRYWWFYLLAVLPAHLLVQLPLPDVTITRV
                     VIQYVLNCGTALIGALALAQFEPGTHRFNRVLPVWRLVFFGGLIAPFTTSLIMAALFV
                     TADVDRNFWLTTVVRALTNSFAILTVVPLMVHTANRLRAPRQAIDVPRVVEGTLLTVC
                     LAVACFVALFAPPLPAVASAAKLCLPLPMLLWAAARFGVAGSSAATLLLGILAICGAL
                     AGTGPFMAEPAAHATLSVVLFLVVTGVPLLLLGAALDERRSLEIAREASDALHGAVLA
                     SIQDQMVVLSSDGRIVDANESWRQLGERIPAAPFVTLKVGENLLHHCIEAGARGDGLA
                     TRLGNCCVEVFGGRALHHRIEYAAHSPNGITWFEVTIEPLRRPEGGAVLVWTDVTYRK
                     QSEAKERAQQQQLVHLGRAAVLGELSGAFAHELTQPLTSILGNAEAALQLLTKPDVDL
                     REIRNMVRDIVADDVRAAEVIQRLRAMLQRGEISCEPVDLTNVVKDVLDLARIDLLTR
                     HVSVDVVFDPYLPLVLGDRIQLQQVVLNLVVNACEAMTSLEVHKRRISITARFEGSSC
                     EVLCAIRDHGTGIASDDLERIFQPFVTTKTQGLGMGLAICRSIIEAHGGRLWAQNEPD
                     GGATFTFSARMGS"
     gene            5472..5849
                     /locus_tag="VEW08_18075"
     CDS             5472..5849
                     /locus_tag="VEW08_18075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ42702.1"
                     /translation="MANRLPLIAVVDDEESVRLALARMLRASSFEVSIYSSGEDFLQS
                     LKTRVPDCAVLDFQMPGLTGRDVQRALSVAKLSLPIIIVTAHDQPSLREKALADGAVA
                     YFTKPLRRDTLIAALAQAIRRDP"
     gene            6004..6666
                     /locus_tag="VEW08_18080"
     CDS             6004..6666
                     /locus_tag="VEW08_18080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYJ42703.1"
                     /translation="MQSVPTVFVLDDDESFVRALDRLLRAEGFATRTWTSATKFLAEH
                     DPSLPGCLVTDLIMPEMSGLELQRKLLAQGCVRPIVFITGRGDMTTAVEGMRRGAVSF
                     LPKPVSRASLVLALREAMSKDASARVHDAERQRVRDLLGSLTPREAQVLELVARGLLN
                     KQIAAALGTAEKTVKVHRGRLMHKMQVKSAAALVQLLSHGDLLQNTPSAAYTPEGDAL
                     RA"
BASE COUNT         1237 a         2158 c         2099 g         1279 t
ORIGIN      
        1 gttcagccgc tgctccttcg acgcgagaat gcgcaggccg actccgcccg cggctagtgt
       61 gtcacttgac cccaggtctt cgaaggaacc cgccaccgat ccaacgccga cgaaagcgac
      121 cgccccaaga cgccaccaga gcggcgcacg gtactcgacc tgcgcggcgt acatggcgtc
      181 gtcgtagtag cgtccgaccg gatagccgcg aaggtcattg gacgatccaa actggcagcg
      241 ctcgaaaaac ggcgtgtcat ccgacgtcgc gcacaaggaa acgcgcgcgg cgaacacgtc
      301 ctggtcgcga aagcctttcc ggtaatggtt gaacgccagc gagtaggtgt cgtagtcgag
      361 atcggcgccg aaggtatcgc gactgaagtt ggtgcgaaac acggcgcggt agccgccgtt
      421 cggctgaaag ctgtggttgc gggaatccca ctccgccgcg acacccaggc cgccgatgtt
      481 gaagtcgatg ccctcttccg ggatcaggcc ctgagtctct tccggccttg gaaagttgac
      541 ggtggcgcgc atatagcgca gccctgcgta gagctcgggc gcgacccgcc gcatcacctg
      601 cacgagtcca ccggcaaggc gctgctcggt ggaaaaatgg aaatcgggtg agatgtcggg
      661 gacgtagagc tcgtactgcg ccagtgcgat tgccaaacca cctagaattc gccaacggtc
      721 ctcggcgaac ttcgcgtcct gcattatcgc gacgccccaa ctgtcggtgt cggtgtagcc
      781 ggcagccacc gcgctcgtgg acaccggtga cttgggatcg atcttgtaga gcatcaacgc
      841 cgcgaccgcg aggccgttcc cgatcgtcgg attgacgatc gggatcggcg ccacgacaaa
      901 actgtatttt ttcttcttct ccgggtcctc gatgggatga gcgacggcgc tatcagtgtt
      961 tatcggatag tcggtaccga aagcaggcgc tcctgcgaga accagagctg cgagacagcc
     1021 acgacatgaa ttgcgcacgc cgctcactgt ctgctcatgc gctcagtagc gcatcacgac
     1081 gccgagcctg aactggtcga agtccgggct cgcattctcg aggtcatacc actgcttttc
     1141 atacgagaga cgcagggagt aaccggtaga caggtcccag cgcacgccaa ggcctgcgtt
     1201 gtacgtgaac gagtcagagg tgcgtgtgtc ctgcaccgtg gtgcagatat agccgtacca
     1261 cggatcccac cagcaaccgg tcacgggacg gccattgggg atattggtgt cgatgaaggc
     1321 ccagccaatg ccggcggtca cgtacggcgt aatggggcca tccacgaaat tgaaactgcc
     1381 tttgatgaac ggcgtgaacg cctcgatttc acccgatacg ccgactgagc cggccggatc
     1441 cagctgaagc gatgcatcat agtcgacgtt cgaccagtcg aggccgaact gcacctcgag
     1501 cttgtcgctg aaacgatatc cgccgtacac cgaaaaaccg atatcgctgt cgaacgcgac
     1561 ggtcgttcca ccatcgaagt cgacgtcatt gctgttcaga taaagaagat cggcaccgaa
     1621 ctcccagcct tcgccgcgcg attgcgcatt ggcgcctgcg gaaagcaggc tcattgccgc
     1681 cgcacacatg aacaaactac ttggtcgcat gactcccact cctgtgttga atcctggaga
     1741 cgaaccgtca tcggtcgccc ttatcgagat gcgacatgga tgttgggagg gagttcacct
     1801 cacacctcat tcgaaatgga ttctgcgacc gcgacggcaa ccgcttcgct ggcacgaaga
     1861 cgaatccatc agatgaaacc gagctgccgc tctcatccgt gacgagcggc tctcgcgctc
     1921 cgggtgcagc gggacgcttg aatgcgcccg ctgcgtactc ccggagatga tcgacccggc
     1981 gttacgactg tcggcgaagc cggaagaacg tcagcatgcc ggcgaaggcc agcagcaacg
     2041 cgccgaggcc agcaaccgtc ggcatcgggc tcgccgtggc cggcatttcg gccgtgctcg
     2101 cagccatggc cggagcggct tcttcgatcg gatacgaggc gagcggctcg gtcggttcat
     2161 gcggctcgag cgagatcacc ggatcggaga ccttcacgta ggccgtgagc ggctggccga
     2221 cggccaggtt gcgcacgctg accggcttgc cgtcgacctt cacgcgaaac tcgggacgag
     2281 tctcgatctt gcgcgtgtcg gatttcgtgc catccgcgag gttgaacttc atgtacacgg
     2341 cattggtgcc ggtgcgcacg aggtcaccct tcaccaccgc gtactgttga ccatctttct
     2401 cgatgatctc cgaacacacg aggcggatgt tgggaaagcg cgcaaggacc gcttcgctga
     2461 actgcgcggt ggagcagtcc gcggccagcg ccggggcgga aacgccaccg agcaccgcgg
     2521 taagtccaat gatcgacagg aaaatggaac gtctcatgat ctctctccgt tagttaggat
     2581 tcgctcggcc attcataagt cgcggcaacg atgaatctct ggggcgcgtt gccgaggtag
     2641 taaaacgggt aacaggtgac cagcgtgaca cccggcatgt cggtgtcggc gagcaggcgc
     2701 ttgtcggtct tctcgacgat gtcgatggaa gtcacgcgat agcggtgcat cttgatcttc
     2761 gtgcgaacct cgatgaggtc gccaaccttg atgtccttga ggacacggaa gaatccgtcg
     2821 cgatggccgg cgatgccgac gtttccgccg gtatcgggcg ttcccatgcc ggcgacgagg
     2881 ccggcgccgc gattgaggct gagctcgtcg tcgtgttcga acaccggaac ttccaacttg
     2941 agtgtcggaa tggtgagcac cgcgacggcc ttcatgggcg actgttgcgc cgcctggtag
     3001 tcccgcaccc tgcccgccgc ccacagagtc tggtccggcg gattcaacga agcggcgaat
     3061 tcttcgatcg cattggtgct gccgagcgcg ctgtcagcct gggcaagcgc gaacagcgaa
     3121 cacagcagcg cgcccgcgag gtaggcggcg gcctccgacc agcgcaatac ccgcgcgagt
     3181 ccgcgcgtgc cattggaggg gccatgtgta ctgtgactca cgccacgact atgaaaagca
     3241 gcgcgccgct tgtgtatggg accttggtcc gatccttccg gggactactg ccggcgcttg
     3301 cgcgggcata ctgcgggcgg cgcagcaggg aaacgatggc cgctcatacc attcactcca
     3361 cggcacacaa ccagagccgt gaccgtttgt taggaaatcc cgcgaccgac cacgcgctgg
     3421 gcgcgctggc gcttgccgcc gggtactacg ctacggcaca gatcggcgaa gcgctggcgt
     3481 ttccgaacgc accagtctcc gcgctctggc tgcccaacgc tttggtcatg ggcgcgctgc
     3541 tgctggcccc gatgcgctac tggtggttct acctgctggc ggtcctgccg gcccatctgc
     3601 tggtgcagtt gcctctgcca gacgtgacga tcacccgcgt cgtcattcaa tacgttctca
     3661 attgtggtac cgcgctgatc ggcgcactgg cgcttgccca gttcgaaccc ggcacgcacc
     3721 ggtttaaccg ggtattgccc gtatggcgac tggtcttctt cggcggcctg atcgcgccgt
     3781 ttacgacgag cctcatcatg gctgccctgt tcgtgaccgc cgatgtggac cgcaacttct
     3841 ggctcaccac ggtggtgcgg gcacttacca acagtttcgc gattctcacc gtggttccgt
     3901 tgatggtgca caccgccaac cgcctgcgtg ccccacgcca ggcgatcgat gttccacgcg
     3961 tggtcgaagg gacgctgctg acggtttgcc tggcggtggc ctgtttcgtg gcgctgttcg
     4021 ctccgccatt acccgccgtg gcctctgcgg cgaagttgtg cctgcccctg cccatgctgc
     4081 tgtgggcggc cgcgagattc ggcgttgccg gcagctccgc ggcgaccctg ctcctcggaa
     4141 tactcgcgat ctgcggagcg ctcgcaggca cgggcccgtt catggcagaa ccggctgcgc
     4201 acgccacctt gtcggtggtg ctgttcctgg tggtcacggg cgtcccgctg ttgcttctgg
     4261 gggccgcgct cgatgagcgg cgctcgctcg aaatcgcgcg cgaggccagc gatgcgctgc
     4321 acggcgcggt gttggcgtcg attcaggacc agatggtcgt tctcagcagc gatggacgca
     4381 tcgtcgatgc caacgagtcc tggcggcagc tcggcgagcg gattccggcc gcgccgttcg
     4441 tcacgctcaa ggtgggcgaa aatctcctgc atcactgcat cgaggctggc gccaggggcg
     4501 acggactcgc gacccggctg ggcaactgct gcgtggaagt ctttggcggc cgcgccctgc
     4561 atcatcgaat cgaatacgca gcccactcgc cgaacggcat cacctggttc gaggtgacta
     4621 tcgaacccct gcgccgcccc gagggcggcg cggttctcgt gtggactgat gtcacttatc
     4681 gcaagcagtc cgaagccaag gagcgcgccc agcagcagca gctcgtgcac ctgggtcgcg
     4741 cagcggtcct gggcgaactc tcgggcgcgt ttgcgcatga actcacccag ccgctgacct
     4801 cgattctcgg caatgccgaa gcggcgctgc agctgctcac caagcccgat gtcgacctgc
     4861 gcgaaatccg caacatggtg cgcgacatcg tcgccgacga tgtccgcgcc gccgaggtaa
     4921 ttcagcgcct ccgcgcgatg cttcagcgtg gcgagatctc gtgtgagccc gtggacctga
     4981 ccaacgtcgt caaggacgtt ctcgacctgg cgcgcatcga tctgttgacg cgacacgtgt
     5041 ccgtcgacgt ggtcttcgat ccctacctgc ccctcgtatt gggcgatcgc atccagttgc
     5101 aacaggtcgt gctcaacctg gtcgtgaacg cctgcgaggc gatgacatcg ctggaagttc
     5161 acaaacggcg gatttccatt accgcccgtt tcgaaggcag cagttgtgag gtcttgtgtg
     5221 ccattcgcga ccacggtacc ggcatcgcca gcgacgatct cgagcgcatc tttcagccgt
     5281 tcgtcaccac gaagactcag ggcctgggca tggggcttgc tatctgccgc tctatcatcg
     5341 aagcgcacgg cggcaggctg tgggctcaga acgaaccaga cggcggcgca accttcacct
     5401 tcagcgccag gatggggtca taggacaaag gtccactatc gactcgcgcc tccggaacgc
     5461 caaatcagga aatggcgaat cgtcttccgc tcatcgccgt cgtcgacgac gaagaatccg
     5521 ttcggctcgc gctcgcacgc atgttgcgcg cgtcgtcgtt cgaggtctct atctacagtt
     5581 ctggcgagga cttcctgcag tctctgaaga cacgcgtgcc cgactgcgcg gtgctcgatt
     5641 tccagatgcc cgggctgacc ggccgcgacg tccagcgcgc attgtcagtc gcgaaactgt
     5701 cacttccgat catcattgtt accgctcacg atcagccatc cctgcgcgaa aaggcgctcg
     5761 ccgacggtgc agtggcctat ttcaccaagc cattgcgccg tgacaccttg attgcagcac
     5821 tggcgcaggc cattcgacgc gacccctagt actacctagt agccagactt ctgtgggacc
     5881 ttggtcttac gttccctggc agtgtctatt ttccgcacgc cgatccataa tccgcgccgg
     5941 catcacaaaa gcgatatgag tcgcgatgct tccgggcacg ggcccgcgga atgggagagg
     6001 tctatgcaat cggtacctac ggtattcgtc ctcgatgacg acgaatcttt cgtgcgcgcg
     6061 ctggacagac tgttgcgcgc cgagggcttc gcaactcgta cgtggacatc agccaccaaa
     6121 tttctcgccg agcacgaccc ttcgctgccc ggctgcctgg tcaccgacct gatcatgccc
     6181 gaaatgagcg ggctcgaact acagcgcaag ttgttggcac agggttgtgt gcgtccgatc
     6241 gtgttcatca ccgggcgcgg cgacatgacc acggctgtcg aaggtatgcg ccggggagcc
     6301 gtcagcttcc tgccgaagcc cgtctcacgc gcatcactgg tgctcgcgct ccgcgaagcc
     6361 atgtcgaagg acgcgtccgc tcgcgtccat gatgccgagc ggcaacgcgt tcgcgacttg
     6421 ctgggctcgc tcacgccgcg cgaagcgcag gtgctcgaac tcgtcgcgcg cggcctgctc
     6481 aacaaacaga ttgccgccgc gcttggcacg gcggagaaga ccgtgaaggt tcatcgcggc
     6541 cggctcatgc acaagatgca ggtgaagtcg gcggcggcgc tggtgcagct gctgagccac
     6601 ggcgacctgc tgcagaacac tccgtccgcg gcatacacgc ccgaaggcga cgcgctgcgc
     6661 gcctgactaa tccgcctggc gctgacctga ccgggagtcg agtttggcga gcgtcgggaa
     6721 catgagctga cgcatctcgt cggaccagtt gtgcgtcatg cggcgataca act
//
LOCUS       DASQJS010000457        10441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3754483, whole genome shotgun sequence.
ACCESSION   DASQJS010000457 DASQJS010000000
VERSION     DASQJS010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3754483"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4240)
                     /locus_tag="VEW08_18085"
     CDS             complement(<1..4240)
                     /locus_tag="VEW08_18085"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HYJ42704.1"
                     /translation="MLGKIPAARLALIEKIVAQALKSVPGARRPLTREFLSAFFRGVA
                     EEDLRAHRASDLAAAALAHLEFGRRRTGSAVLVDIATPLAADASTASHRALLRVVAPD
                     MPFLVDSIGIVFSQMNIAVHLIVHPVLAVRRDSRGALKTVGIDGNSQLESWQMMEIDR
                     PRDSEAAAEIERRVRAALEDVRRAVADFRAMLERIRAVANELERTPLPVPKSHASEAR
                     ALLAWMHDGHFVFLGYRHYHLKRGRSRDALVRDDASGLGILRSRKPAAPILLTGHLRQ
                     QARAPELLVLTKANTASTVHRGSYLDYVGVKTFDASGQVNGEHRFLGLWTSSAYHKPP
                     AEIPLLRRKLDAVIAHFGLPAQSHDAKSVVNVIETFPRDELFQTPVGELIASVRGIVN
                     LYERRRVRLFVRRDSFERFYSCLVYVPRDRYNTEVRERIERIVRQRFGGTHVETQVQI
                     SDSMLARLHMLVRTPQGARTVENIPAIEAEIAAAAATWEDRLQQALISRGVERSAVEL
                     AARYAKTFPPAYRADVEPAQALEDIADLEALAADPTVPQLNLRAAHKQGNLSLRILRA
                     NEPISISDILPMLENFGLRVLAEHPYQITTSERAVWIQDFELDARDLRRADIAALEPL
                     FKEAFLAAWRGDIENDGFNRLLLCAGLSARDIVVVRAYCRYLLQTGIPFSQAYMERVL
                     VAQAPITAALVRLFQTQFALANKARESAAERITKGIRRALDKVASLDEDRILRSYLTV
                     IRATLRTNYYQTADGTADGTPKSWVSFKLNPHAIPELPLPRPKYEIFVYSPRVEGVHL
                     RMGDVARGGIRWSDRREDFRTEVLGLMKAQHVKNTVIVPVGAKGGFYPKRLPAGGSRD
                     DVQKEVVASYQTFIRGLLDVTDNIVNGKVVVRPGVVRRDGDDAYLVVAADKGTATFSD
                     IANAISIEYGHWLGDAFASGGSAGYDHKGMGITARGAWECVKRHFRELGLDIQSQSFT
                     CAGVGDMSGDVFGNGMLLSKHTLLVAAFDHRHIFLDPNPNAAAGFRERERLFKLPRSS
                     WDDYTRKLISRGGGVWPRSAKSIPLSAETQKLLDLAMPSATPAEVMRAILHMRVDLLW
                     NGGIGTYVKATDESHAEVRDRANDAIRADGREIRARVIGEGGNLGCTQRGRVEYAQSG
                     GPEKSGGRINTDFIDNSAGVNTSDLEVNIKILLSDVERRGKLTRAARNKLLASMTDEV
                     AGLVLRNNYLQTQALSVLEQRAPERLTEYQGLIRSLERNGHLDRAIEFLPGDDEFLER
                     RKKRLGLTRPELSVVLAYSKIWLSNHLLDSDLPDDPYFASEVQRYFPAPMRKRYSREI
                     PRHRLRREIVATATTNSLVNRMGPVFVTRAQEDTEADPASISRAYTIAREIFSMRSVW
                     ADIESLDNSVPADVQYGMFYRASRLLRHTSYWLLR"
     gene            complement(4305..4769)
                     /locus_tag="VEW08_18090"
     CDS             complement(4305..4769)
                     /locus_tag="VEW08_18090"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HYJ42705.1"
                     /translation="MTRREKLHLEGFLPYRLSVLSNTVSSAIAAAYFANFGLSIPEWR
                     VMAVLAANPGLVAAEVTARTAMDKVAVSRAVATLLSAGRLQRTTAPADRRRTHLALTA
                     AGARVYAQVVPMALKYERQLVAPLSKSELATLDRILRVLLGRAVELGPAHVP"
     gene            4846..5898
                     /gene="hppD"
                     /locus_tag="VEW08_18095"
     CDS             4846..5898
                     /gene="hppD"
                     /locus_tag="VEW08_18095"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017725398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HYJ42706.1"
                     /translation="MVTPSLTNPMGTDGFEFVEYAAPDPQLLRSLFERLGFPVVARHR
                     SKNVTLHSQGDINFVINAEPGSFGQQFAKLHGPSVCAMAFRVNDAAAAYERAISLGAE
                     PHRGSVGPMELNIPAIVGIGGSLIYFVDRYGERSIYDVDFKPVVPEPLERAGLLHIDH
                     LTHNVHRGHMDKWTGFYETMFNFREIRYFDIEGKKTGLFSRALTSPCGKIRIPINESQ
                     DDKSQIEEYLREYRGEGIQHIALATEDIYRTVDVLRGRGVAFQDTPETYYEGVGGRVT
                     GHDESIEELRKRRILIDGNGANDEGILLQIFTANVIGPIFFEIIQRKGNQGFGEGNFK
                     ALFESIELDQIRRGVI"
     gene            5895..6545
                     /gene="maiA"
                     /locus_tag="VEW08_18100"
     CDS             5895..6545
                     /gene="maiA"
                     /locus_tag="VEW08_18100"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HYJ42707.1"
                     /translation="MSLELYTYWRSSASYRVRIALNLKSLPYVMHGVNLVREGGDQWS
                     VAYREVNPQSRVPTLVHDGQRFTQSLAIIEYLDETFPDHRLIPRDPVDRARVRMLSQI
                     IACDIQPLQNTSTTQYLKEKLRLDEAAITAWLREWIVRGLDAYNAHLQRDHLSGKFSH
                     GDTPTMADCCLVPQLYAASRFGVEVTRYPRLALIAENCAAMSAFQHAHPSKQPDAV"
     gene            6547..7260
                     /locus_tag="VEW08_18105"
     CDS             6547..7260
                     /locus_tag="VEW08_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HYJ42708.1"
                     /translation="MAGKKVCADAASALAGLTRDGMTVMSGGFGLCGIPENLIVALRD
                     SGARNLTVISNNAGVDGFGLGLLLETRQIRKMVSSYVGENREFERQYLAKELELEFNP
                     QGTLAERIRAGGAGIYGFYTRTGAGTIVADGKEQRTVNGELFVLETGLTADLAIVKAW
                     KGDTEGNLVYRKTARNFNPMMATAGRVTVAEVEELVEPGALDPDQIHTPGIFVQRIFR
                     GTNYQKRIEQRTVRKKGAA"
     gene            7260..7895
                     /locus_tag="VEW08_18110"
     CDS             7260..7895
                     /locus_tag="VEW08_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HYJ42709.1"
                     /translation="MPWTREDLAKRAARELRDGYYVNLGIGIPTLVANYIPGGMDVVL
                     QSENGMLGMGPFPYEGEEDPDLINAGKQTITELATSSFFSSADSFGMIRGGHINLSVL
                     GALEVAENGDLANWMVPGKMVKGMGGAMDLVAGVRRVVVVMDHNSKDGGPKFKPRCEL
                     PLTGANCVDMLITDLAVFERPDRSSPFKLLELAPGVTAEQVRARTSAKFET"
     gene            complement(7917..8282)
                     /locus_tag="VEW08_18115"
     CDS             complement(7917..8282)
                     /locus_tag="VEW08_18115"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYJ42710.1"
                     /translation="MTKANPFNASCPSQDILALIGSKWSMLLMCILRDGPTRPGELAR
                     RTGGISQKMLTQTLRELERHGIVSRLDYGTVPPRVEYSLTPLGQSLAGLVRQIESWVE
                     SNYPRLSRAARSYDSNATP"
     gene            8345..9421
                     /locus_tag="VEW08_18120"
     CDS             8345..9421
                     /locus_tag="VEW08_18120"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HYJ42711.1"
                     /translation="MWKSTEVTRRLKLDAPIVQGPFGSGLSAVDLVVAVSEGGGLGSF
                     GVHHLDGAGIRAIDAQIRARTRRPYALNLWIPLGDSDDPRMTDAQWAAAIDLLRPYFA
                     ELKVPLPVRPARFGPHYQEQIETLLELRPPVFSFVFGVPSPDVLERCRSAGVATLGAA
                     TTPAEARLLADSGVDMIVASGFEAGGHRVSFLQEPEDCLTGTMALIPQVVDAVKVPVI
                     AAGGIADGRGIAAALRLGAAAAQVGTAFLACEESNATPLHREKLFSVDARRTTLTRAF
                     TGRLARSIHNDFIDALKGKEPLLAPYPVHAWLTAQLRSAALAANRADVISLWAGQGAP
                     LLKHRRASELLQSLVCETGEAMRS"
     gene            complement(9461..>10441)
                     /gene="zapE"
                     /locus_tag="VEW08_18125"
     CDS             complement(9461..>10441)
                     /gene="zapE"
                     /locus_tag="VEW08_18125"
                     /inference="COORDINATES: protein motif:HMM:NF015902.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HYJ42712.1"
                     /translation="EAAAAPLGKRLLRGLTSNRAAPKGVYLWGGVGRGKTWLMDLFYS
                     SLTLAAKRRTHFYRFMQEVHADLKRLKGMQSPLDGVADKIAKKARVICFDELFVSDIA
                     DAMILAGLFSALVKRGVALVFTSNVRPKDLYKNGLQRDRFVPTIALIEKQCDVVAVDG
                     GVDYRLRQLTAAPIYLPSGDAATQKQLEELFDDLSDDDVESGGTITVHNRKIKVVRES
                     ENVIWFDFAALCEGPRSPADYVAIASEYQSVLIANVPVFDDATDNAARRFISVIDEFY
                     DRGVNVIVSAAAAPVDLYRGEKLKFEFQRTSSRLVEMQSKEYLARAHRAA"
BASE COUNT         1838 a         3248 c         3500 g         1855 t
ORIGIN      
        1 cgcgaagcag ccagtagctg gtgtgacgca gcagccggct ggcccggtag aacatgccgt
       61 actgcacatc cgcgggcacc gaattgtcca gcgactcgat gtcggcccac accgaacgca
      121 tggagaagat ttcacgcgcg atggtgtatg cgcgcgagat cgaggccgga tccgcttcgg
      181 tatcttcctg cgcgcgggtc acgaaaaccg gacccatgcg gttcaccagg ctgttggtgg
      241 tggcggtggc gacgatctcg cgtcgcagcc ggtgccgcgg aatctcgcgc gaataacgtt
      301 tgcgcatggg ggcggggaag tagcgctgaa cttcgctcgc gaagtaggga tcgtcgggca
      361 gatctgaatc gagcaggtga ttgctgagcc agatcttgct gtacgcgagc accacggaga
      421 gctcaggacg cgtgagcccg aggcgcttct tgcgacgctc gaggaattcg tcgtcgcccg
      481 gcaggaattc gatggcccgg tccaggtggc cattgcgctc cagtgagcga atcaggccct
      541 ggtattcggt cagccgctcg ggggcgcgct gctcgagcac cgacaatgcc tgggtctgca
      601 ggtagttgtt ccgcagcacg aggccagcca cttcgtccgt catgctggcc agtagtttgt
      661 tgcgcgcggc gcgcgtcagt tttccgcggc gctcaacgtc cgagagcagg atcttgatgt
      721 tcacttcgag gtcggaagtg ttcacgccgg cggagttgtc gatgaagtcg gtattgatgc
      781 gcccgccgga cttttccggt cctccggact gggcgtattc cacacggccg cgttgcgtgc
      841 agccgaggtt cccgccttca ccgatcaccc tggcgcggat ttcacgccca tcggcgcgga
      901 tggcgtcatt ggcgcggtcg cgtacttccg catgcgattc gtccgtggcc ttcacgtagg
      961 tgccgatgcc gccgttccac agcaggtcga cgcgcatgtg caggatggcg cgcatgactt
     1021 ccgcgggcgt ggccgacggc atggcgagat cgagcagttt ctgcgtctcg gccgagaggg
     1081 gaatcgactt ggcgctgcgc ggccacactc cgccgccgcg cgagatgagt ttgcgtgtgt
     1141 agtcgtccca gctcgatcgc ggcagcttga acaggcgttc gcgctcgcga aagccggccg
     1201 cggcgttggg atttggatca aggaagatgt gccgatgatc gaacgcggcg accagcaagg
     1261 tgtgtttcga cagcagcatg ccgttgccga acacatcgcc ggacatgtcg ccgacgccgg
     1321 cacaagtgaa ggattgggac tggatgtcca gacccagctc gcgaaagtgc cgcttgacgc
     1381 attcccaggc gccgcgcgcg gtaatgccca tgcccttgtg gtcgtagccc gccgagccac
     1441 ccgaggcgaa cgcgtcgcct aaccaatggc cgtactcgat ggagatggca ttggcgatgt
     1501 cggagaacgt ggcggtgccc ttgtcggcgg ccacgaccag gtaggcgtcg tcgccgtcgc
     1561 gacgcacgac cccggggcgt acgacaacct tgccgttcac gatgttgtcg gtgacgtcga
     1621 gaaggccgcg gatgaaggtc tggtaactgg cgacgacctc cttctgcacg tcatcgcgcg
     1681 atccaccggc gggcagacgc ttcgggtaga agccgccctt ggcgcccacg ggcacgatga
     1741 ccgtgttctt gacgtgttgc gccttcatga gccccagcac ttcggtgcgg aagtcttcac
     1801 gccggtccga ccagcgaatg ccgccgcggg ccacgtctcc catgcgcagg tgcacgcctt
     1861 cgacgcgcgg gctgtacacg aagatttcgt acttcgggcg gggcagcggc agctcgggga
     1921 tggcgtgcgg attgagcttg aacgaaaccc agctcttcgg agttccatcc gccgtcccgt
     1981 cggccgtctg atagtagttg gtgcgcagcg tcgcgcggat caccgtgagg tagctgcgca
     2041 ggatgcggtc ttcgtcgaga ctcgccacct tgtcgagcgc gcgccggatg cccttggtga
     2101 tgcgctccgc ggcgctttca cgcgccttgt tggccagggc gaactgcgtc tggaacaggc
     2161 gcaccagcgc cgcggtgatg ggggcctgcg ccaccagcac gcgttccatg taggcctggc
     2221 tgaacgggat gccggtctgc agcaggtagc ggcaataggc gcgtaccacc acgatgtcgc
     2281 gtgcgctcag gcccgcgcac agcagcaggc gattgaagcc atcgttttcg atgtcgccac
     2341 gccaggcggc caggaaggct tccttgaaca agggttccag cgccgcgatg tcggcacgcc
     2401 tgaggtcgcg cgcatcgagt tcgaaatcct ggatccacac tgcgcgctcg gacgtagtga
     2461 tctggtacgg gtgttcggcc aatacgcgca gcccgaagtt ttcgagcatg ggcaggatgt
     2521 cggaaatgga aatcggctcg ttcgcccgca ggattcgcag cgaaaggttg ccctgcttgt
     2581 gggcggcgcg caggttgagc tgcggaacgg tgggatcggc ggcgagtgcc tcgagatcgg
     2641 caatatcttc gagcgcctgg gcgggctcga cgtcggcgcg ataggcgggc ggaaacgttt
     2701 ttgcgtaacg tgcggcgagt tccacggcgc tgcgttccac gccgcgcgag atcagcgcct
     2761 gctgcaggcg gtcttcccag gtggccgccg ccgcggcgat ctcggcttcg atggccggga
     2821 tgttttcgac cgtgcgcgca ccctggggcg tgcgcaccag catgtgcagg cgggccagca
     2881 tggagtcgga gatctgcacc tgcgtttcga cgtgcgtgcc gccgaatcgc tgccgcacga
     2941 tgcgctcgat gcgctcgcgt acctcggtgt tgtaacggtc gcgcggcacg tacacgagac
     3001 acgagtagaa gcgttcgaag ctgtcacggc gtacgaacag gcgcacgcgc cgccgctcgt
     3061 agagattgac gatgccgcgc acgctggcga tgagctcgcc cacgggagtc tggaacagtt
     3121 cgtcgcgcgg gaaggtctcg atgacgttga ccacggactt ggcgtcgtgg ctctgcgccg
     3181 gcaggccgaa gtgcgcgatg accgcatcga gcttgcggcg gagcaatgga atttccgccg
     3241 gcggcttgtg ataggcgctc gatgtccaca ggccgaggaa gcgatgttcg ccattcacct
     3301 ggccggaagc atcgaaagtc ttcacgccga cgtagtcgag atagcttcca cgatgcacgg
     3361 tggacgccgt gttggccttg gtgagcacaa gtagttcggg cgcgcgtgcc tgctgacgca
     3421 ggtgcccggt gagcaggatg ggtgctgccg gtttgcgcga ccgcaggatg ccgaggccgc
     3481 tggcgtcgtc gcgcaccagc gcgtcgcgcg agcggccgcg cttcaggtga tagtggcgat
     3541 agccgaggaa cacgaagtgt ccgtcgtgca tccaggcgag cagggcacgc gcttcactgg
     3601 cgtgggattt cggcacgggc aggggcgtgc gttcgagctc gttggccact gcgcggatgc
     3661 gttcgagcat cgcgcgaaaa tcggccacgg cccggcgcac gtcttcgagc gcggcgcgga
     3721 cgcggcgttc gatctccgcg gccgcttcgc tgtcgcgcgg ccggtctatc tccatcatct
     3781 gccaggattc gagctgtgaa ttgccgtcga tgccgaccgt cttgagcgcg ccacgcgaat
     3841 cgcggcgcac cgccagtacc ggatggacga tgagatggac ggcgatgttc atctggctga
     3901 acacgatgcc gatggaatcc acgaggaagg gcatgtccgg tgccaccacg cgcaacagtg
     3961 cacgatgcga cgcggtggac gcatcggccg caagtggagt cgcgatgtcg acgagcaccg
     4021 cactgccggt gcgccgacgt ccgaattcca ggtgggcgag tgccgcggcc gccaggtccg
     4081 acgcacggtg cgcgcgcagg tcttcctcgg cgacgccccg gaagaaggcg ctgagaaatt
     4141 cccgggtcag cgggcgccgc gcgcctggca cgctcttcaa ggcttgcgca actatcttct
     4201 cgatgagcgc gaggcgcgct gcggggatct tgccgagcat aagtactctt tcggtgagag
     4261 cgcgcaggat cagggcgcgc gattatacat gcggccgctg acgctcacgg cacgtgcgcg
     4321 ggaccgagtt cgacggcgcg gcctagcaaa acccgcagaa tccggtcgag cgtggcgagt
     4381 tcggacttcg acagtggcgc cacgagctga cgttcgtatt tcagcgccat gggcacgacc
     4441 tgcgcgtaga cacgcgcacc ggccgcggtg agcgccaggt gcgtgcggcg gcggtcggcc
     4501 ggcgccgtgg tgcgctgcag gcggcctgcg gacagcagcg tggccaccgc gcggctcacg
     4561 gcaaccttgt ccatggcggt acgggcagtc acttccgctg cgaccagacc gggattcgcg
     4621 gccaacaccg ccatgactcg ccattcgggt attgaaagcc cgaagttggc gaaataggcg
     4681 gccgcaatag ccgaactcac cgtattggac agtacggaca gccggtaagg cagaaatcct
     4741 tccaggtgga gcttctcgcg tcgcgtcatg ggctgcttgc gcctttcgca ttccggcgga
     4801 atggttacaa ttgaaactaa atcaatttcg caccggagac cagtgatggt cactccttcc
     4861 ctgacaaatc ccatgggcac cgacgggttc gagttcgtcg aatacgccgc ccccgacccc
     4921 cagctgttgc gcagcctgtt cgagcgcctg gggttcccgg tggtggcccg ccaccgttcc
     4981 aagaatgtga cgctgcactc gcagggcgac atcaatttcg tcatcaacgc ggagccgggt
     5041 tccttcggcc aacagttcgc gaagctccat ggcccctcgg tttgcgccat ggcgtttcgc
     5101 gtgaacgacg ccgccgccgc ctacgagcgc gccatttcgc tcggcgccga gccgcatcgc
     5161 ggcagcgtgg gtcccatgga gctcaacatc ccggccatcg tcggcatcgg cgggtcgctc
     5221 atctacttcg tggatcgcta cggtgagcgc tcgatttatg acgtggactt caagccggtc
     5281 gtcccggagc cgctggagcg cgccggcctg ctgcacatcg accacttgac ccacaacgtg
     5341 catcgcggac acatggacaa gtggaccggc ttctacgaaa cgatgttcaa cttccgcgaa
     5401 atccgctatt tcgacatcga aggcaagaag accggactgt tctcgcgcgc cctgaccagc
     5461 ccctgcggca agatccgcat accgatcaac gagtcgcagg acgacaagtc gcagatcgag
     5521 gaatacctgc gcgaatatcg cggcgagggc atccagcaca tcgcgctggc cacagaagac
     5581 atctatcgca ccgtcgacgt gctgcgcggc cgcggagtcg cgttccagga cacacccgaa
     5641 acctactacg aaggtgtcgg cggccgtgtc acaggtcacg atgagagcat cgaagagttg
     5701 cgcaagcggc gcatcctcat cgacggtaac ggcgccaatg acgaaggcat cctgctgcag
     5761 atattcacgg cgaacgtcat cggcccgatc tttttcgaaa tcatccagcg caagggaaac
     5821 cagggtttcg gcgagggaaa tttcaaggca ttgttcgaat ccatcgaact cgaccagatt
     5881 cgccgggggg tcatttgagt ctcgagctct acacttattg gcgcagctca gcgtcatatc
     5941 gcgttcgcat cgcgctcaat ctcaaatcgt tgccctatgt catgcatggc gtgaacctgg
     6001 tgcgcgaggg cggcgatcaa tggtcggtgg cctaccgtga agtcaacccg caaagccgcg
     6061 tgcccacgct ggtgcacgac ggtcagcgtt tcacccagtc cctggccatc atcgaatacc
     6121 tggacgagac ctttccggat catcggctga ttccgcgtga cccggtggac cgcgcgcgcg
     6181 tgcgcatgct ctcgcagatc attgcctgcg acatccagcc gctgcagaac accagcacca
     6241 cgcagtacct caaggagaag ctgcgtctcg acgaggcggc gatcaccgcc tggctgcgtg
     6301 aatggatcgt tcgcgggctc gacgcctaca acgcacacct gcaacgggat cacctgagcg
     6361 gcaagttttc ccatggtgat acgccgacga tggcggactg ctgcctcgtg ccgcagctgt
     6421 atgccgcgtc gcgcttcggg gtcgaggtca cgcggtatcc gcgcctggcg ctgatcgcgg
     6481 aaaactgcgc ggccatgtcc gcgttccagc acgcgcatcc gtcaaaacaa cccgatgcgg
     6541 tctgacatgg ccgggaaaaa agtgtgcgcg gacgcggcgt cggcgcttgc ggggttgacg
     6601 cgtgacggca tgacggtgat gtcgggcggc ttcgggctgt gcggcatccc ggaaaatctc
     6661 atcgtcgcgc tgcgtgactc gggcgcgcgc aatctcacgg tcatctccaa caacgcgggc
     6721 gtcgatggtt tcggcctggg gttgctgctg gagacgcggc agatacgcaa gatggtgtcg
     6781 tcatatgtcg gggagaaccg cgagttcgaa cgccagtacc tggcgaagga gctagagctc
     6841 gaattcaatc cgcagggaac gctggcggag agaattcgtg ccggcggtgc gggaatctat
     6901 ggtttctaca cgcgcaccgg cgcgggcacg atcgtcgccg acggcaagga gcagcgaacc
     6961 gtgaacggcg agttgttcgt gctcgaaacc gggctcaccg cggacctggc catcgtcaag
     7021 gcctggaagg gcgataccga aggcaacctc gtctatcgca agaccgcgcg caatttcaac
     7081 ccgatgatgg caacggccgg ccgcgtcacg gtcgccgagg tcgaggaact ggtggagccc
     7141 ggtgcgctgg accccgacca gatccacacc ccgggaattt tcgtgcagcg gatcttccgc
     7201 ggaacgaact accagaagcg catcgagcag cggacggttc gcaagaaggg agccgcgtaa
     7261 tgccctggac ccgcgaagac ctggcgaaac gcgcggcgcg cgagctgcgt gacggctact
     7321 acgtgaacct cggcatcggc atcccgacgc tcgtggctaa ctacattccg ggcggcatgg
     7381 acgtggtgct gcagtcggaa aacggcatgc tcggcatggg tccgtttccg tacgagggag
     7441 aagaagatcc cgacctcatc aacgccggga agcagaccat caccgaactc gccacctcgt
     7501 catttttctc gtcggccgac agcttcggca tgattcgcgg cggccacatc aacctgtcgg
     7561 tgctgggtgc gctcgaagtc gctgaaaacg gtgacctcgc gaactggatg gtgcccggga
     7621 agatggtgaa gggcatgggc ggggccatgg acctcgtggc cggcgtgcgt cgcgtggtgg
     7681 tggtgatgga tcacaattca aaggatggcg ggcccaagtt caagccgcgc tgcgaattgc
     7741 cgctgaccgg cgcgaactgc gtggacatgc tcatcacgga cctcgccgtt ttcgagcgac
     7801 cagatcgatc cagcccgttc aagctactcg agctggcgcc gggcgtcacc gcggaacagg
     7861 tgcgcgcgcg gacttcggcg aagttcgaaa cctgatacca cttgccgggc ctggcgtcag
     7921 ggagtcgcgt tcgagtcgta tgaacgcgcg gcccgcgaca ggcgcgggta gttggattcc
     7981 acccagctct cgatctgcct caccaggccg gcgagcgatt ggccgagcgg cgtgagcgag
     8041 tattcgaccc gaggcggaac cgtgccgtag tcaaggcgcg acacgatgcc gtggcgttcg
     8101 agttcgcgca gcgtctgcgt gagcatcttc tgcgaaatgc cgccggtgcg ccgcgcgagt
     8161 tcgcccgggc gcgtcggccc gtcacgcagg atgcacatca gcagcatgga ccacttgctg
     8221 ccgatgagcg cgaggatgtc ctgggagggg caactggcgt tgaacggatt tgctttggtc
     8281 atgttggcag gcaccaaaag gtacgtactt atgagatgga gcgcatgagt ttactcttgc
     8341 ggccatgtgg aagagcacgg aagtcacccg ccgcctgaag ctcgacgcac ccatcgtgca
     8401 ggggccattt ggcagcggcc tgtcggcggt cgatctcgtc gtcgcggtga gcgagggcgg
     8461 ggggctcgga tcgttcgggg tgcaccacct ggatggcgcg ggaattcgcg cgatcgacgc
     8521 gcaaatccgc gcgcgcacac ggcgcccgta cgcgctgaac ttgtggattc cgctcggcga
     8581 cagcgacgat ccgcgcatga ccgacgcgca gtgggcggct gcgatcgacc tgttgcggcc
     8641 gtacttcgcc gaactcaagg ttccgctgcc cgtgcggccg gcgcgattcg ggcctcacta
     8701 ccaggaacag atcgagacgc tgctcgagct gcggccgccc gtattcagct tcgtgttcgg
     8761 cgtgccgtcg ccggacgtgc tcgagcgctg ccgcagcgcc ggagttgcca cgctgggggc
     8821 ggcgaccacg cctgccgaag ccagactact ggccgactcg ggcgtcgaca tgatcgtcgc
     8881 ctccggtttc gaggcgggcg ggcaccgcgt gtccttcctg caggagccgg aggattgcct
     8941 cacgggaacc atggcgttga ttccgcaagt ggtagatgcc gtgaaggtgc cggtgatcgc
     9001 cgctggcggc attgccgatg ggcgcggaat cgccgcggcg ttgaggctgg gcgccgccgc
     9061 cgcgcaggtc ggcaccgcat ttctcgcctg cgaggagtcg aacgcgacgc cgttacatcg
     9121 cgaaaagctt ttcagcgtgg atgcgcgccg cacgacgctg acacgtgcat tcacagggcg
     9181 cctggcgcgc agcattcaca acgatttcat cgacgcgttg aagggcaagg agccgctgct
     9241 cgcgccgtat ccggtgcatg cctggctgac cgcgcagctc aggtccgcgg cgctggccgc
     9301 gaatcgtgcc gatgtgatct cgctctgggc ggggcagggg gcaccgctgc ttaagcaccg
     9361 gcgtgcaagt gaactgttgc aatcgctcgt ctgcgaaacc ggggaggcaa tgcgcagctg
     9421 aggcgcgtcc ccgtttgcca gcaaatcagg aatgccccca ttacgcggcg cggtgcgccc
     9481 ttgccagata ttccttgctc tgcatctcga cgagcctgct cgaagtgcgc tgaaattcga
     9541 acttgagctt ctcgccgcga tacagatcaa cgggcgccgc cgcggcggag acgatcacgt
     9601 tcacgccacg atcgtagaac tcgtcgatca ccgagatgaa gcgacgcgcc gcgttgtcgg
     9661 ttgcgtcatc gaacaccggc acattggcta tcaacacgga ctggtactcg ctggcgatgg
     9721 ccacgtagtc cgccgggctg cgcggacctt cgcacaacgc cgcgaaatcg aaccagatga
     9781 cgttttcgct ctcgcgcacc accttgatct tgcggttgtg cacggtgatc gtcccgccgg
     9841 attcgacgtc gtcgtcggaa agatcgtcga acagctcctc gagctgcttc tgcgtcgccg
     9901 cgtcgccgct cggcaggtag atgggcgcgg cggtgagctg gcgcaagcgg tagtccacgc
     9961 cgccgtcgac cgcgaccacg tcgcattgtt tctcgatcag ggcgatggtc ggcacgaagc
    10021 ggtcgcgctg caggccgttc ttgtagagat ccttgggtct gacgttcgac gtgaacacca
    10081 gcgccacgcc acgcttgacc agggcggaga acaggcccgc gaggatcatc gcgtccgcga
    10141 tgtcggagac gaacaattcg tcgaagcaga tcacacgcgc cttcttcgcg atcttgtcgg
    10201 cgacgccgtc gagcggcgat tgcatgccct tcagccgttt gagatcggcg tgcacttcct
    10261 gcatgaaacg gtagaagtgc gtacgccgct tcgccgcgag cgtgaggctc gagtagaaca
    10321 gatccatgag ccacgtcttg ccgcggccca cgccgcccca gagatagaca cctttcggcg
    10381 cggcgcggtt gctggtcagc ccgcgcaaca gccgctttcc gagcggcgcc gcggccgctt
    10441 c
//
LOCUS       DASQJS010000458         3405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2502864, whole genome shotgun sequence.
ACCESSION   DASQJS010000458 DASQJS010000000
VERSION     DASQJS010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2502864"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VEW08_18130"
     CDS             complement(<1..418)
                     /locus_tag="VEW08_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYJ42713.1"
                     /translation="MTKLNVNGNVVEVDVDPETPLLWVLRDSLGLTGTKFGCGMALCG
                     ACTVHLEGQPVRSCQIPVSAIGDKPVTTIEGLSKDRSHPVQKAWIEHDVPQCGYCQSG
                     QIMSASALLETKPRPSDADIDAAMAGNICRCGTYQRI"
     gene            569..1615
                     /locus_tag="VEW08_18135"
     CDS             569..1615
                     /locus_tag="VEW08_18135"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYJ42714.1"
                     /translation="MSEISPKPPIVTEVVIIGAGPCGLFQVFELGLLGIGCHLVDSLA
                     SAGGQCTELYPDKPIYDIPALPTCGAQELIDRLLQQISPFNAPLHLGQEVTALQKRED
                     GRFSVETITGTRFDAGAVVIAGGVGSFQPRRIGVPGADLFEGKAIHYRVRDAASYHGK
                     KLAIFGGGDSALDWVIDFAGKASAVTHIHRRPEFRGAPASVAKMHSLVEQGAVRYIEG
                     LAEGLRVEGETLTGVNVKGSDGTLHAVDADHVFAFFGLHPKLGPIAEWGMELEKKALR
                     VDTEKFQTSIPGIFAVGDINTYPGKKKLILSGFHEAALAAFAIQHHLYPAKKQFLQYT
                     TTSPVMQKRLGVPH"
     gene            1638..2093
                     /gene="azu"
                     /locus_tag="VEW08_18140"
     CDS             1638..2093
                     /gene="azu"
                     /locus_tag="VEW08_18140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HYJ42715.1"
                     /translation="MKYIRTIACLSLVFAAPGVFAADKVCKVDIASTDQMTFDKKEIA
                     VAADCTEVEVTLKHTGKMPAAAMGHNWVLSKTADAQAVANDGLSAGVANDHVKKGDAR
                     AIAHTKIIGGGQTTSVKFSTSALKKGEAYTFFCSFPGHVALMKGTFKFG"
     gene            2097..2966
                     /gene="tesB"
                     /locus_tag="VEW08_18145"
     CDS             2097..2966
                     /gene="tesB"
                     /locus_tag="VEW08_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HYJ42716.1"
                     /translation="MNQQLADLQQQFQLERLEVNLFRGQSRDTGSPQVFGGQVLGQAL
                     MAAHSTIDDGRVVHSMHAYFLRRGDFTKPIVYSVDRSRDGGSFSARRVVAVQDGEQIF
                     ISSASFQKPEKGLEYQFTAPKVPPPEELEAMSKPPQAELDKLPEKLRRWLEIKRPFEF
                     RPVQKYNPLAPVASEPVRQIWMRAVDKLPDDDTLHHCLLAYVSDYWLLDTSTMPHGSS
                     FLRGNLVMASIDHAIWFHRRARVDDWLLYSLDSPSSSGARGFARGSFYSRNGTLVAST
                     AQEGLIRLVQPQP"
     gene            3011..>3405
                     /locus_tag="VEW08_18150"
     CDS             3011..>3405
                     /locus_tag="VEW08_18150"
                     /inference="COORDINATES: protein motif:HMM:NF017966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0017009 - protein-phycocyanobilin linkage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpcT/CpeT family chromophore lyase"
                     /protein_id="HYJ42717.1"
                     /translation="MAPPADDRDALAQFWTGIYDMTEERVASNPPDSPLEPRTTRVQI
                     NVRRVTLPWMGTHILYVEEFPFDRPFEARRRALLSIEPAPGTDGSLRVRQYTRSDGTG
                     NPGTLASSDVESVSGCDIFLKREGTQFRGG"
BASE COUNT          631 a         1086 c         1074 g          614 t
ORIGIN      
        1 ggattcgctg gtaggttcca caccggcaga tgttgccggc catggcggcg tcgatatcgg
       61 cgtcggaggg tcgcggcttg gtttccagca gcgcagaagc cgacatgatc tggccggact
      121 ggcaatagcc acattgcggc acgtcatgtt cgatccacgc cttctgtacc gggtggctgc
      181 ggtccttcga gagtccctcg atggtcgtca ctggcttgtc gccaatggcg ctgaccggga
      241 tctggcagga gcgcaccggc tggccctcga gatgcacagt gcaggcgccg cacagggcca
      301 tgccgcatcc gaatttggtc cccgtcaggc ccagcgagtc gcgcaggacc cacagcagcg
      361 gggtttccgg atcgacatcc acttcgacca cgttgccatt gacgttgagc ttggtcacga
      421 gcgaatcccc tggttttcgg cgactgtggc gactatatgc cggtcgcgtt gtttgtagga
      481 gggggccagc ttcctggaca ggtgagtgtt cagcctgggt tatgattgcg cccgctaaac
      541 tggccccagc tccttgaagg aattgagttt gtccgagatc tccccgaagc cgcccatcgt
      601 caccgaagtc gtgatcattg gcgccggccc atgtggtctg ttccaggttt tcgagctcgg
      661 cctccttggt atcggctgcc atctggtcga ttcgctggcc tccgctggcg ggcaatgcac
      721 cgagctctat ccggacaaac ccatctatga catcccggcg ctgcccacct gtggcgccca
      781 ggagctcatc gatcgtctgc tgcagcagat ttcgccgttc aacgcgccgc tgcacctggg
      841 acaggaagtg accgcgctac agaaacggga agacggccgt ttcagcgtcg agaccatcac
      901 cggcacacgt ttcgacgcgg gtgcggtggt gatcgccggt ggcgtcggct cgttccagcc
      961 gcggcgcatc ggcgtcccgg gcgcagatct cttcgagggc aaggccatcc actatcgcgt
     1021 gcgcgacgcg gcgagctacc acggcaagaa gctcgcgatc ttcgggggcg gtgattcggc
     1081 gctcgactgg gtcatcgact tcgcgggcaa ggcctcggcg gtcacacaca tccatcgccg
     1141 cccggaattc cgcggagcgc ccgcgtccgt ggcgaaaatg cattcgctgg tcgagcaggg
     1201 agcggtgcgt tatatcgagg gcctggcaga aggattgcga gtggagggtg agacgctgac
     1261 cggggtcaat gtaaaaggca gcgatggcac gctgcacgcc gtcgacgccg atcacgtgtt
     1321 cgcattcttc gggttgcacc cgaagctcgg gcccatcgcc gaatggggca tggaactcga
     1381 gaagaaagcc ctcagggtgg acaccgaaaa gttccagacc agcatccccg gcatctttgc
     1441 cgtgggagac atcaacacct atccgggtaa gaagaaactg attctctccg gatttcacga
     1501 agccgcgctt gcggccttcg cgatccagca tcatttgtat cccgccaaga aacagttcct
     1561 gcagtacacg accaccagcc cggtcatgca gaagcgcctt ggcgttccac actaactacc
     1621 tctcaacgga gcccaagatg aaatacatac gcacgattgc ctgcctgtcc ctggtgtttg
     1681 ccgcgcccgg tgtcttcgcc gccgacaagg tctgcaaggt cgatatcgcg agcaccgacc
     1741 agatgacgtt cgacaagaag gaaattgccg tcgccgccga ctgcaccgag gtcgaagtca
     1801 cgctcaagca caccggcaag atgccggcgg cggccatggg ccacaactgg gtgctgtcga
     1861 aaaccgccga tgcgcaggcg gttgccaatg acggtctctc cgctggtgtc gccaatgacc
     1921 acgtcaagaa aggcgatgcg cgcgcgattg cgcacaccaa gatcatcggt ggcggccaga
     1981 ccacgtcggt gaagttctcg acttcggcgc tcaagaaggg cgaggcatac acgttcttct
     2041 gcagcttccc cgggcacgtc gcgctcatga aaggcacgtt caagttcggt tagtcgatga
     2101 accagcagct cgccgatctc cagcagcagt tccaactgga gcggctcgag gtgaacctgt
     2161 ttcgcggcca gagccgcgat acgggcagcc cgcaggtgtt cggcgggcag gtgctgggcc
     2221 aggcattgat ggcggcgcat tccaccatcg atgacgggcg cgtcgtgcat tcgatgcacg
     2281 cgtatttcct gcggcgaggc gacttcacca agcccatcgt ctacagcgtc gatcgcagcc
     2341 gcgacggcgg cagcttctcg gcgaggcgcg tggtggcagt gcaggacggc gagcagatct
     2401 tcatcagctc ggcgtcgttc cagaagcccg agaaggggct cgagtaccag ttcacggcac
     2461 cgaaagtgcc gccgccggaa gagctcgagg ccatgtcgaa accaccgcag gccgagctcg
     2521 acaagctgcc ggagaaactg cgccgctggc tcgaaatcaa acggccgttc gaattccgcc
     2581 cggtgcagaa atacaacccg ctggcgccgg tggccagcga gccggtacgg cagatctgga
     2641 tgcgcgcggt ggacaaacta cccgacgacg acacgctgca tcactgcctg ctggcgtatg
     2701 tctccgacta ctggctgctc gacacttcga ccatgccgca cggctcctcg ttcctgcgcg
     2761 gcaacctggt gatggcgagc atcgaccacg cgatctggtt tcaccggcgc gcgcgcgtcg
     2821 atgactggtt gctgtacagc ctcgacagcc cgagcagttc cggcgcacga ggtttcgcgc
     2881 gcggcagctt ctattcccgc aacggaaccc tcgtggccag cactgcgcaa gaggggctca
     2941 tacgtctcgt gcaaccgcag ccctgatcgg tctggccgcc tgcgccggct ccctgggcgc
     3001 gacgccgccg gtggcgccac ccgccgatga ccgtgacgcg ctggcgcagt tctggaccgg
     3061 catctacgac atgaccgaag agcgggttgc gagcaacccg ccggactcgc cactcgagcc
     3121 acgaaccacc cgcgtgcaga tcaacgtgcg acgcgtgacg ttgccgtgga tggggacaca
     3181 tatcctctat gtcgaagagt ttcccttcga ccggccattc gaggcccggc ggcgcgcatt
     3241 gctgtcgatc gagcctgcgc cgggcaccga tggatccctg cgagtgcggc aatacacccg
     3301 cagcgacggg actggcaatc ccggcacact ggcttcgtcc gacgtggagt ccgtgtccgg
     3361 ctgcgatatt ttcctgaagc gcgagggaac gcagtttcgc ggtgg
//
LOCUS       DASQJS010000459         3727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_4074522, whole genome shotgun sequence.
ACCESSION   DASQJS010000459 DASQJS010000000
VERSION     DASQJS010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4074522"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VEW08_18155"
     CDS             <1..237
                     /locus_tag="VEW08_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42718.1"
                     /translation="DFGDGNTDTSGIVLRGGYTVARNWTINATLFLNELSNDVPQSVT
                     VFNEATPAPYDTTVVTGINDREYKRLQLDLNFRF"
     gene            369..1757
                     /gene="pstC"
                     /locus_tag="VEW08_18160"
     CDS             369..1757
                     /gene="pstC"
                     /locus_tag="VEW08_18160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HYJ42719.1"
                     /translation="MTPAALLIALTLLSVGAFWIGKTRSLALVGGTRGVRHLHSLPAH
                     YGLMTAMWCALPALAVIGLWLTFQDAIILRLVVDHLPAGVRDLPANDLSLVMNDVRNL
                     IAGNVPASSVSESVRAAADEYVALRGISRMALTVLVMVMAIAAIIVIRSRITPELRAR
                     NGVERVIEWILIACSTIAVFTTLGIVASVAFEAFRFFAMVSPIDFLFGTSWSPQMAIR
                     ADQVGSSGSFGAVPLFAGTALISFIAMAIAVPIGLFSAIYLAEYAHPKFRTVAKPLLE
                     VLAGVPTVVYGFFAALTIGPALRGWGESFGLNVASESALAAGFVMGIMIIPFVSSLAD
                     DMISAVPQGLRDGAYALGATRSETIKQVVLRAALPGIVGGVLLAVSRAIGETMIVVMA
                     AGLAANLTANPLEAVTTVTVQIVTLLVGDQEFDSPKTLAAFALGLVLFLVTLALNVLA
                     LHIVRKYREQYE"
     gene            1741..3048
                     /gene="pstA"
                     /locus_tag="VEW08_18165"
     CDS             1741..3048
                     /gene="pstA"
                     /locus_tag="VEW08_18165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HYJ42720.1"
                     /translation="MNSTNKPRRTPAETQAIVESSLRRRYWAERRFRAYGFTAVAAGM
                     AFLVYLFVVIFSNGIGAFQQTQMRVSVFYDPQVIDPTGSRNPDDLHAADYPALLRAAL
                     KAKFPEVEGRAATRELMRLVSGSAAFDLQKRVTSDPALIGTTEERWLLASDAVDLLQK
                     GNIDRTIPEAERPLSDQQLAWTDKLKAEGSLALKFNKTFVTGGDSREPENAGIWGAVV
                     GSFFMLALTLLLSFPIGVATAIYLEEFARRSRWTDLIEVNINNLAAVPSIVFGLLGLA
                     VFIGFFGMPRSAPLVGAMVLTLLTLPTIIIASRAALKAVPPSIREAALGMGASKIQMV
                     AHHVLPLAMPGMLTGTIIGMARALGESAPLLMIGMVAFIVDIPGGVTDAATALPVQVF
                     LWSDSPERGFVEKTSGAIIVLLAFLVVMNTTAVILRKRFERRW"
     gene            3053..>3727
                     /locus_tag="VEW08_18170"
     CDS             3053..>3727
                     /locus_tag="VEW08_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HYJ42721.1"
                     /translation="MEAAQVDRAGLNLKPSGRQPNESSNASLQSPVESGKTVGNIAVD
                     DPIFSCRDVDVYYGAKHAIRKVGIDVAKKQVLAMIGPSGCGKSTFLRCLNRMNDTIEG
                     ARVEGSILLDGKDIHDRKQDVVQLRARVGMVFQKPNPFPKSIYENVAYGPRIHGLAGD
                     RVELDEIAHSSLQRAGLWDEVKDRLQAPGTSLSGGQQQRLCIARTIAVNPEVILMDEP
                     CSALDPI"
BASE COUNT          642 a         1197 c         1166 g          722 t
ORIGIN      
        1 gacttcggtg acggcaatac cgacacctcc ggcatcgttc tgcgcggcgg ctacaccgtg
       61 gcgcgcaact ggaccatcaa cgccacgttg ttcctcaacg agttgtccaa tgacgttccc
      121 cagtcggtga ccgtgttcaa cgaggcgact cccgcgccgt acgacacgac cgtggtcacc
      181 gggatcaatg atcgtgaata caagcgctta cagctggatc tgaacttccg gttctaagtg
      241 agaacccagg ggcggcaggg atgccgcccc ttcattttca cgaagctgta acaaagcggc
      301 gctaaacagg gtagccctgt gcgacagcga cccgggctaa cgccctgcca ggaaacccga
      361 acaagactat gactccggcg gctctactca tcgcgctcac gcttctgtcc gtgggcgcct
      421 tctggattgg caagacacgt tcgctcgccc tcgtgggtgg gacgcgtggc gtgcgacacc
      481 tgcactcgct gccggcgcat tacggactca tgacggccat gtggtgtgcg ctgccggcgc
      541 tcgcggtcat cggcctgtgg ctcactttcc aggacgcgat catcctgcga ctggtcgtcg
      601 atcacctgcc tgccggggtc cgcgatctgc cagcgaacga tctttcgctc gtcatgaacg
      661 acgtgcgcaa ccttatcgct ggaaacgtgc cggcgagttc agtcagtgaa tcggttcgcg
      721 ccgccgcaga cgaatacgtc gctcttcgag gcatcagccg catggcgctc accgtgctgg
      781 tcatggtgat ggcgattgca gcaatcatcg tgattcgttc gcgcattact cccgagctgc
      841 gcgcgcgtaa tggcgtggaa cgcgtcatcg aatggattct tatcgcctgc tcgacgatcg
      901 ccgtgttcac cacgctcggc atcgtcgctt cggtcgcatt cgaagcgttc cgcttcttcg
      961 ccatggtgtc gccgatcgac ttcctgttcg gtacatcgtg gagtccccag atggcgatcc
     1021 gcgccgacca ggtgggttcg tccggatcct tcggggctgt gccgctgttc gcaggcacgg
     1081 cgctcatctc gttcattgcc atggccatcg ccgttccgat cggcctgttc tcggccatct
     1141 accttgccga gtacgcgcat ccgaaattcc gcaccgtggc caagccgctg ctcgaggttc
     1201 tggccggcgt accgacggtc gtgtacgggt tcttcgcggc gttgaccatt ggtccggcgc
     1261 tgcgtggctg gggcgagagc ttcggtctca acgtggcatc ggaaagcgcg ctcgcggccg
     1321 gtttcgtaat gggcatcatg atcatcccgt tcgtgtcttc gctcgccgac gacatgatca
     1381 gcgccgtacc ccagggcctg cgcgacggtg cgtacgcgct cggcgcgacg cgttcggaaa
     1441 ccatcaagca ggtagtgctg cgcgctgcgt tgcccggcat cgtcggcggc gtgctgctgg
     1501 ccgtgtcgcg cgccatcggc gagaccatga tagtggtgat ggcggcgggg ctggccgcca
     1561 acctcaccgc gaatccgctc gaggccgtga ccaccgtgac ggtgcagatc gtcacgctgc
     1621 tcgtcggcga ccaggagttc gacagcccca agacactcgc ggcattcgcg ctcggcctgg
     1681 tgctgttcct cgtcacgctg gcactcaacg tgctcgcgct gcacatcgtc aggaaatacc
     1741 gtgaacagta cgaataagcc gcgccggact ccggcggaga cacaggccat cgtcgagagc
     1801 agcctgcgcc ggcgttactg ggcggagcgc cgctttcgcg cctacggttt caccgcggtg
     1861 gcggccggta tggcgttcct tgtctatctg ttcgtcgtca tcttctcgaa cggcatcggc
     1921 gcgttccagc agacgcagat gcgagtgtca gtcttttacg acccgcaggt catcgatccg
     1981 accggcagtc gaaatcccga tgatctgcac gccgctgatt acccggcgct gcttcgcgcg
     2041 gcgctcaagg cgaaatttcc cgaggtcgaa ggccgggcgg caacgcgcga gctcatgcgc
     2101 ctggtgagtg gaagtgcggc gttcgatctg caaaagaggg tgacctcgga ccccgcgttg
     2161 atcggcacga ccgaggagcg ctggctgctc gcgagcgatg ccgtcgacct gctgcagaaa
     2221 ggcaacatcg atcgcacgat tcccgaggcg gagcgcccgc tgagcgacca acagctggca
     2281 tggaccgaca aactcaaggc cgaaggttcg ctcgccctca agttcaacaa gacgttcgtg
     2341 acgggaggcg attcgcgcga gcccgaaaat gccggcatct ggggcgcggt ggtgggttcg
     2401 ttcttcatgc tggcgctgac gctcctgctg tcgttcccga tcggcgtcgc tacggctatc
     2461 tacctcgagg agttcgcgcg ccgcagccgc tggaccgacc tcatcgaggt gaacatcaac
     2521 aatctcgccg cggtgccgtc catcgtgttc ggcctgctcg gccttgcggt attcataggc
     2581 ttcttcggca tgccgcgctc ggcaccgctg gtgggtgcga tggtgctcac gctgctgact
     2641 ttgccgacca tcatcatcgc gtcgcgcgcg gcgctcaaag ccgtgccgcc ttcgattcgc
     2701 gaagcggcgc tcggaatggg tgcgtccaag atccagatgg tggcgcacca cgtgctgccg
     2761 ctggccatgc ccggcatgtt gaccggcacc atcatcggca tggcgcgcgc gctgggcgaa
     2821 tccgcaccgc tgctcatgat cggcatggtg gccttcatcg tcgacatccc gggcggcgtc
     2881 acggatgcgg ccaccgcgct gcccgtgcag gtattcctgt ggtccgacag ccccgagcgc
     2941 ggcttcgtcg agaagacatc aggcgccatc atcgtgctcc tggcctttct cgtggtcatg
     3001 aacaccactg cggtaatcct tcgtaaacga ttcgaaagac gatggtgaaa atatggaagc
     3061 ggcacaagtg gatcgagcgg gtctgaatct gaagccaagc ggccggcagc cgaacgagag
     3121 ttcgaacgct tcgctgcagt cccccgtcga gtccggcaag accgtgggca acatcgcggt
     3181 cgacgatccc atcttcagct gccgtgacgt cgacgtgtat tacggcgcga aacacgccat
     3241 caggaaggtc ggcatcgacg tcgccaaaaa gcaggtgctg gcgatgatcg ggccgtcagg
     3301 ttgcggaaag tcgacgttcc tccgctgcct caatcgcatg aacgacacga tcgaaggtgc
     3361 gcgagtcgaa ggttccatcc tgctcgacgg caaggacatc cacgacagga agcaggatgt
     3421 ggtgcaactg cgtgcccgcg tcggcatggt gttccagaag ccgaatccgt tcccgaagtc
     3481 catctacgag aacgtcgcct acggtccgcg gatccacggc ctggccggcg accgcgtcga
     3541 gctcgacgaa atcgcgcaca gctcgctcca gcgcgcgggc ttgtgggatg aagtgaagga
     3601 ccggttgcag gcccccggga cttcgctgtc cggcggccag cagcagcgtt tgtgcatcgc
     3661 ccgcacgatt gccgtaaatc ccgaagtgat tctcatggac gagccatgct cggcgctgga
     3721 ccccatc
//
LOCUS       DASQJS010000460         4652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_308353, whole genome shotgun sequence.
ACCESSION   DASQJS010000460 DASQJS010000000
VERSION     DASQJS010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308353"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2504)
                     /locus_tag="VEW08_18175"
     CDS             complement(<1..2504)
                     /locus_tag="VEW08_18175"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-like domain-containing protein"
                     /protein_id="HYJ42722.1"
                     /translation="MNRTKLALALGTALVVTGCFFDYPDPTVDPATITLNDLAVHDPS
                     VIRADDGSFYVFGSHLAAARSTDLMNWQYIANGVDAANPLWSTIPLDGTQWTGIPGSW
                     AADVIKLRDGKYRFYYNFCGIPPGGQCNGPRSYLGVAISDRIDGPFIDQGIFLRSGMT
                     PAEIAAGYGPEGVTSYDARIQPNTIDPDVFYDANGKLWMVYGSYSGGIFILEMDETTG
                     KPVPGQGYGRHLAGGDHSAIEGGYMLYSPQSRYYYLFMSFGGLAANDGYNIRIARSKT
                     PSGPFLDAEGRDMVNARGGFDSIAPFGVKLMGGFNFASAVGDTETARGYLSPGHNSAY
                     YDAASKKHLLITHTRFPNRGEGHAIRVHEMYVNKDGWLVASPHRHVPIQGDNVVDTGD
                     VLGDYKLINLGKDINRTAKQSVYVSLNANGTITGDVVGKFKRDVTNPDRITIYLQGTP
                     ELFEGVLQWQWNEAAGALTPIFTAVSSKGVSVWGSRMALRSPQDVVADVAAALTLPTA
                     AKDNSLTLPTVGARGTSIVWTSSNASIIDVDGTVTRPNVGAGDQVVMLTANLKFRGQT
                     TTRSFQVTVPQRQPFNRVAQFDFENSLIESLGRFGQGQPTGNRIFNAGTVSFDAGHLG
                     QALRLNGSNGVRLPSGLISNYQYTVSFWINPTAITRFTPGFFAAVNERVDGAGFPYST
                     QWLSFLPESWDGNTMLWSGSDQWFDGSAGLRIQPDAWRHLAFSVNKGVVSVFIDGVRR
                     FNAGTIADFFSTQSGTFALGVNYWDLPFNGMIDELKFYEAAMSAQEIRALDIDHLSNP
                     ELLASAASLLDLGDLTAVRENLTLPRSGAYASAISWA"
     gene            complement(2572..>4652)
                     /locus_tag="VEW08_18180"
     CDS             complement(2572..>4652)
                     /locus_tag="VEW08_18180"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HYJ42723.1"
                     /translation="DSVEISGDAVTLRGSLPSPALWTAETPYLYSVKLSLLRGKASLH
                     ELVQRFGFRTFEVRPRDGVYLNGRKIVLKGINRHSFDTKTGRTLTRAQNYADVRLIKG
                     ANMNAVRMSHYPPDPAFLEAADELGLYVLDELAGWQGFYDTPTGARLIGQIVRRDVNH
                     PSILFWDNGNEGGWNRENDGEFDRWDPQRRPVLHPWAIHSGINTDHYENYDSTVRLSA
                     GPDIFMPTEFLHGLYDGGIGAGFRDYWDVMGRSPTVAGGFFWAFADEGIERTDQKGRI
                     DNMGNSAPDGIVGAHHEKEGSYFAVKEIWSPVELRELSFAGSRVNMTVANHYDFNSLG
                     QCAVLWRAIRTPAAGARGKVVTIGEGRAPLPDLAPRTSATWQLPVGVSALGANELIEI
                     TALDPRGQDLWTWVVRGAATVPRDADKAQPHVRRQGNTVTSGGYALEFDPATGALVKL
                     TTHGRSVPLQGPRLAAWQRAPKLRSFIDVAGTSTLRKLELAPDIDTLARAQYDGALSE
                     VSWKVRGDELIVSYRIAYEGAADILGVTFDYPEKNVLGKRWVGEGPYRIWKNRLAGTT
                     FGLHEANYSRSTPGVSYQYPEFEGFFGAWSWLELRTRAGNVVIRNSGNIPYFGLYAPT
                     PGEKPILELPHLGWSFLHAVPPIGTKFALPDVLGPQSQPARFTSPVEGQLSLTFQGFR
                     LPGPRYEDPY"
BASE COUNT          820 a         1434 c         1531 g          867 t
ORIGIN      
        1 gcccagctga tggcgctggc ataggcgccg ctgcgcggca gcgtgaggtt ttcgcgcacc
       61 gcggtcaggt caccgagatc gagcagcgat gccgccgacg ccagcagctc tggattggac
      121 aggtggtcga tatcgagcgc ccggatttcc tgggcgctca tcgccgcctc atagaatttg
      181 agctcgtcga tcatgccgtt gaagggcaga tcccagtagt tgacgcccag cgcaaaggtg
      241 ccggactggg tgctgaaaaa gtccgcgatg gtgcccgcgt tgaaacgccg gacgccgtcg
      301 atgaacaccg acaccacacc cttgttcacc gagaacgcca aatgcctcca ggcgtcgggc
      361 tggatacgca agccggcgct gccgtcgaac cactggtcgc tgccgctcca cagcatggtg
      421 ttgccatccc agctttccgg caggaacgac agccattgcg tggaatacgg aaagcccgcg
      481 ccatccacgc gttcgttcac cgccgcgaag aaccccgggg tgaagcgcgt gatagccgtt
      541 ggattgatcc agaacgacac cgtgtattgg tagttgctga tgagcccgga gggcaggcgc
      601 acgccgttgc tgccgttcag ccgcaaagcc tggccgagat gacccgcgtc gaagctcacc
      661 gtgcccgcgt tgaatatgcg gttacccgtg ggttggccct gtccgaagcg tccgagcgat
      721 tcgatgagtg aattctcgaa atcgaactgt gccacgcgat tgaacggttg gcgctgcggc
      781 acggtcacct ggaagctccg ggtggtagtt tggcctctga acttgagatt ggcggtcagc
      841 atgactacct ggtcgccggc acccacgttc ggacgcgtga ccgtgccgtc gacatcgatg
      901 atgcttgcgt tgctcgaagt ccagacgatg ctcgtgccgc gcgcgccgac cgtcggcagc
      961 gtgagcgaat tgtcttttgc cgcggtgggc agggtgagcg cggcagcgac atccgccacc
     1021 acgtcctgcg gcgagcgcag ggccatgcgt gatccccaca ccgacacgcc cttcgacgac
     1081 accgccgtga agatgggcgt gagcgctccg gcagcctcgt tccattgcca ttgcagcacg
     1141 ccttcgaaga gctcgggagt gccctggagg tagatcgtga tgcgatcagg gttcgtgaca
     1201 tcgcgcttga atttgccgac gacgtctccc gtgatggttc cattggcatt cagcgaaacg
     1261 tacaccgatt gcttcgccgt cctgttgatg tccttgccga gattgatgag cttgtagtcg
     1321 ccaaggacgt cgccggtatc gaccacgttg tcgccctgga tgggcacgtg gcgatgcggc
     1381 gaagcgacga gccagccgtc cttgttcacg tacatttcgt gcacgcggat cgcgtgacct
     1441 tcgccacgat tggggaagcg ggtatgcgtg atgagcagat gtttcttcga cgctgcgtcg
     1501 taataggccg agttgtggcc gggcgagagg tacccgcgcg cggtctcggt gtcaccgacc
     1561 gccgaggcga aattgaaacc acccatgagc ttcacgccga acggggcgat gctgtcgaaa
     1621 ccgccgcgtg cattgaccat gtcgcggcct tccgcgtcga ggaacgggcc gctgggagtt
     1681 ttcgagcgcg cgatgcggat gttgtagccg tcgttcgcgg ccaggccgcc gaacgacatg
     1741 aacaggtagt agtagcgcga ctgcgggctg tacagcatgt agccaccctc gatggcgctg
     1801 tgatcgccgc cggcgagatg cctgccgtat ccctggccgg gaaccggctt gcccgtggtc
     1861 tcatccattt ccaggatgaa gatgccgccc gaatacgagc cgtagaccat ccacagcttg
     1921 ccgttcgcgt cgtagaacac gtcggggtcg atggtgttgg gctggatgcg cgcgtcgtag
     1981 ctcgtgacgc cttcgggtcc gtagcccgcg gcaatttccg cgggcgtcat gccggagcgc
     2041 aggaagatgc cctggtcgat gaacggtccg tcgatgcgat cggaaatcgc gacgccgaga
     2101 tatgaacgcg ggccattgca ctggccaccc ggcgggatgc cgcagaagtt gtaatagaac
     2161 cggtacttgc cgtccctgag cttgatgacg tccgcggccc acgagccggg aatgccggtc
     2221 cactgcgtgc cgtcgagcgg aatggtgctc cacagcggat tcgcggcatc cacgccgttg
     2281 gcgatgtact gccagttcat gaggtcggtg ctgcgcgccg cggcgagatg cgagccgaac
     2341 acgtagaaag aaccgtcgtc ggcgcgaatc accgaaggat cgtgcaccgc caggtcgttg
     2401 agggtgatgg tggccggatc gaccgtggga tccgggtagt cgaagaagca gccggtgacc
     2461 acgagcgccg tgcccagggc aagcgcgagt ttcgtcctgt tcatcgatcc ccctaactac
     2521 cggcgattct tcctgccggg cggggatcgt aatactcgga caaatggctg gctaatacgg
     2581 gtcttcgtat cgcggaccgg gcaatcggaa tccctggaac gtaagactca actggccttc
     2641 gaccgggctc gtgaaccgcg ccggctggga ctgcggcccc agcacatccg gcagcgcgaa
     2701 cttggtgccg atcggcggaa cggcgtgcag gaacgaccag ccgagatggg gcagctcgag
     2761 gatgggcttt tcacccggcg tcggcgcata caggccgaag taaggaatgt tgccgctgtt
     2821 gcggatgacc acgttaccgg cgcgagtacg cagctcgagc cagctccacg caccgaagaa
     2881 tccctcgaac tcgggatatt ggtaactgac gccaggcgtc gaacgcgagt agtttgcttc
     2941 gtgcaggccg aacgtggttc ccgccaggcg attcttccag atgcggtacg ggccttcacc
     3001 cacccagcgt ttgccgagca cgttcttttc gggatagtcg aacgtcacac cgagaatgtc
     3061 ggccgcacct tcgtaggcga tgcggtagct cacgatcagc tcgtcgccgc gaaccttcca
     3121 cgacacttcg ctcagggcgc cgtcgtattg cgcgcgagcg agcgtgtcga tgtccggcgc
     3181 gagctcgagc ttgcgcagcg tcgaagtgcc ggcgacatcg atgaagctgc gcagcttcgg
     3241 tgcgcgctgc cacgcggcga ggcgcgggcc ttgcagcgga acactgcgac cgtgcgtggt
     3301 gagcttcacg agcgcgccgg tggccggatc gaattcgagc gcgtagccgc cggaagtcac
     3361 ggtgttgccc tgcctgcgga cgtgcggttg cgccttgtcc gcgtcgcgcg gaacggtcgc
     3421 ggcgcctctg actacccagg tccacagatc ctggccgcgc ggatcgagcg ccgtgatttc
     3481 gatcagttcg ttcgcgccga gtgcgctgac gcccacgggg agttgccagg tagcgctggt
     3541 gcgcggcgcc agatccggta gtggcgcgcg gccttcgccg attgtgacga ccttgccgcg
     3601 cgcgccggcg gcgggtgtcc ggatcgcgcg ccacagtact gcgcactggc ccaggctgtt
     3661 gaagtcgtag tggttcgcaa ccgtcatgtt cacgcggctg ccggcaaagc tgagttcgcg
     3721 caattcaacg ggcgaccata tttccttcac cgcgaagtag ctgccttcct tctcatggtg
     3781 cgcgccgacg atgccgtcgg gcgccgagtt gcccatgttg tcgatgcggc ctttctgatc
     3841 ggtgcgctcg atgccttcgt cagcaaaagc ccagaagaac cctccggcca cggttggact
     3901 ccgacccatc acatcccagt agtcgcgaaa gcccgcgccg atgccgccgt cgtaaagtcc
     3961 gtgcagaaat tcggttggca tgaagatatc gggcccggcg ctcaatcgaa ccgtgctgtc
     4021 gtagttctcg tagtgatccg tgttgatgcc gctgtggatc gcccagggat gcagcaccgg
     4081 ccggcgctgc gggtcccagc ggtcgaactc gccgtcgttc tcgcggttcc agccgccttc
     4141 gttgccgttg tcccagaaca ggatgctggg gtggttcaca tcgcgccgca cgatctggcc
     4201 gattagccgc gcgccggtgg gcgtgtcata gaagccctgc cagccggcga gctcgtcgag
     4261 cacgtaaagc ccgagctcgt ccgccgcctc gaggaacgcg ggatcgggcg gatagtgcga
     4321 catgcgcacc gcgttcatgt tggcgccctt gatgagtcgc acgtcggcgt agttctgcgc
     4381 gcgcgtcagc gtgcggccag tcttggtatc aaagctgtgg cggttgatgc ccttgagcac
     4441 gatcttgcgg ccattgagat acacgccatc ccgcgggcgg acttcgaagg ttcgaaatcc
     4501 aaagcgctgc accagctcgt gcagcgaagc cttgccgcgc agcagcgaaa gcttcacgga
     4561 gtaaagatac ggagtttcgg cagtccacag cgccggcgat ggcagcgaac cgcggagcgt
     4621 gacggcatcg cccgaaattt ccacagaatc aa
//
LOCUS       DASQJS010000461         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2820264, whole genome shotgun sequence.
ACCESSION   DASQJS010000461 DASQJS010000000
VERSION     DASQJS010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2820264"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VEW08_18185"
     CDS             complement(<1..194)
                     /locus_tag="VEW08_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42724.1"
                     /translation="MPSEKQYDASKPSKLLRVGAFAGLAFMHIPVFVIILYAFTTEDR
                     TYQFPPPGFTLHWFEVAFGRA"
     gene            complement(184..1095)
                     /locus_tag="VEW08_18190"
     CDS             complement(184..1095)
                     /locus_tag="VEW08_18190"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYJ42725.1"
                     /translation="MTVRAASTFFYRRPNWYLALLLVPPLLWFGTVYLGSLFALLAQS
                     FYRIDDFTAQIVREPTLATYQQLVTQPANVDIVLRTLIMAVAVTIACAVIALPIATYM
                     ARYARGRMKAFFYVGVMLPMWTSYLVKVYAWRLLLAKEGIIAWVTAKLGLTGLLEAAL
                     AAPGVGGPSLSSSYIGMFIVFVYMWLPFMILPMAAALERVPGSLLQASADLGARPAQT
                     FRNVVLPLAVPGIAAGSVFTFSLTLGDYIIPSVVGAPGYFIGMMVYVQQGTAGNIPLA
                     AAFSVVPIILIFAYLSIAKRFGAFDAL"
     gene            complement(1092..2129)
                     /locus_tag="VEW08_18195"
     CDS             complement(1092..2129)
                     /locus_tag="VEW08_18195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYJ42726.1"
                     /translation="MTSAIEFRGVSRVFGDVRAVDDVSFTIAPGEFFAMLGPSGSGKT
                     SCLRLVAGFDSPDRGQVLLDGQDVSAVPPYERNVNTVFQDYALFPHMTVLENVAYGPR
                     VRGVDAATRAKRAREMLDLVQLGAQGERRPAQLSGGQRQRVALARALINQPKVLLLDE
                     PLGALDLKLREEMQIELKSLQRRLGITFVYVTHDQGEALSMADHVAVFNKGRIEQLAA
                     PRELYTQPTTAFVARFVGGANVAEGALARAIGGSERPFAIRAENVVVQSESSPAAPNS
                     VSAPGAVVAVQYHGAASRWQVKLDAGEVWSALITEDDSRALNGLAVGARVRLSWPRAA
                     AVTLSESPSVS"
     gene            complement(2235..>3167)
                     /locus_tag="VEW08_18200"
     CDS             complement(2235..>3167)
                     /locus_tag="VEW08_18200"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYJ42727.1"
                     /translation="GDASWPPPGNAPYDLVTASGDASLRLIRGGTVIPIDIGRVPSYN
                     TIDPRLQQAAWHYVDGKHYGVPYQWGPNVLLYNTVTFKTPPTSWSVVFEPQKLPDGKA
                     NKGRVQAYDGPIYIADAALYVKAKKPELGIKDPYELNEAQYAAALDVLRGQHPLIQRY
                     WHDANVQVQDFQNEGIVASGSWPFQANTLLMNKAKVATTVPVEGATGWADTTMLAAGA
                     RHPNCAYKWLEWSISPKVQGDVAAWFGSNPVVPKACEGNELLGAEGCKANGIENFDKI
                     AFWRTPEAKCTSNVEGCVPYSRWVSDYVAIIGGK"
BASE COUNT          601 a         1010 c         1024 g          532 t
ORIGIN      
        1 gcgcggccga acgccacttc gaaccagtgc agcgtgaagc ccggtggcgg gaattgatag
       61 gtgcgatctt ccgtggtgaa cgcatacaga atgatgacga acaccgggat gtgcatgaac
      121 gcaagtccgg cgaacgcgcc cacgcgcaac aactttgacg gtttgctggc gtcatattgt
      181 ttttcagagg gcatcgaagg ccccaaagcg cttggcgatg gatagatagg cgaagatcag
      241 gatgatcggc accaccgaga acgccgccgc gagcgggatg ttgcccgccg tgccctgctg
      301 cacgtacacc atcatgccga tgaaatatcc gggcgcgccc acgacgctcg gaatgatgta
      361 gtcgccgagc gtcagcgaga atgtgaaaac cgatccggcg gcgatgccgg gcaccgcgag
      421 cggcagcacc acgttacgaa acgtttgcgc cggtcgcgct cccaggtcgg cggaggcttg
      481 cagcagcgaa ccgggcacgc gctcgagtgc ggccgccatg ggcaggatca tgaacggcag
      541 ccacatgtag acgaacacga tgaacatgcc gatgtaggag gacgacagcg acgggccgcc
      601 gacacccggc gcggcgagcg ccgcttccag caacccggtg agcccaagct tggccgtgac
      661 ccaggcgatg atgccctcct tcgcgagcag cagccgccac gcgtacacct tcacgaggta
      721 gctggtccac atgggcagca tcacgccgac atagaagaac gccttcatgc ggccgcgcgc
      781 gtagcgcgcc atgtaggtgg cgatgggcag cgcgatgacc gcgcaggcga tcgtcaccgc
      841 gacggccatg atcagcgtgc gcagcactat atcgacgttg gcgggttgcg tgacgagttg
      901 ctggtacgta gccaacgtcg gctcgcgcac gatctgcgca gtgaagtcgt cgatccggta
      961 gaaactctgc gcgagcagcg cgaacaacga gccaaggtag accgtgccaa accacaacag
     1021 cggtggcacg agcagcagcg ccaggtacca gttcgggcgt cggtagaaga acgtcgacgc
     1081 cgcgcgaact gtcatgaaac gctcggggac tcgctgagcg tcacggccgc cgcgcgcggc
     1141 caggaaagcc ggacgcgcgc acccaccgcg aggccgttca gcgcccggga gtcgtcttcg
     1201 gttatcaggg cactccagac ttctcctgcg tcgagcttca cctgccagcg actcgcagcg
     1261 ccgtgatatt gcacagccac cacggccccg ggagcgctca ccgaattcgg cgctgcgggc
     1321 gaggattcac tctggacgac gacgttttcc gcgcgaatgg cgaatggtcg ttccgagcca
     1381 ccgatggccc gcgcgagcgc gccttcggcg acgttggcgc cgcctacgaa acgcgcgacg
     1441 aacgcggtgg tgggctgcgt gtacaactcg cgcggtgccg cgagctgctc gatcctgccc
     1501 ttgttgaaaa ccgccacgtg gtccgccatc gacagggctt cgccctgatc atgcgtgacg
     1561 tagacgaacg tgatgccgag acggcgctgc agactcttga gctcgatctg catttcttca
     1621 cgaagtttca ggtcgagcgc gccgagcggt tcgtcgagca gcaacacctt gggttggttg
     1681 atgagtgcgc gcgccagcgc cacgcgctga cgttgcccgc ccgacaactg cgcgggacga
     1741 cgctcgccct gcgcaccgag ctgcacgaga tcgagcatct cgcgggcgcg tttggcccgg
     1801 gtcgcggcat ccacgccacg gactcgcgga ccgtacgcca cgttctcgag caccgtcatg
     1861 tgcgggaaca aggcgtagtc ctggaagacc gtgttgacgt ttcgttcgta gggcggcacg
     1921 gcggagacgt cctgcccatc gagcagaacc tgcccgcggt cgggcgagtc gaagcccgcc
     1981 accagccgca gacaggaagt cttgcccgaa cccgacggac ccagcatcgc gaagaactcg
     2041 ccgggcgcga tcgtgaacga cacgtcatcg accgcgcgca catcgccgaa cacgcgcgag
     2101 acgccgcgga attcgatggc tgaggtcatg ttgtgtgggg acggcgttgt gtggggacag
     2161 ttgttgtgtg gggacagatt taaagtctgt ccccacccgt ctactgtccc cactcgatca
     2221 ttgtccccac ccgattactt accgccgatg attgccacgt aatcgctgac ccagcggctg
     2281 taaggcacac acccttcgac gttcgatgtg cacttggctt cgggcgtacg ccagaaggca
     2341 atcttgtcga agttttcgat gccgttggcc ttgcagccct cggcgcctaa cagctcattg
     2401 ccttcgcagg ccttcggaac cacgggattc gaaccgaacc acgcggccac gtcgccctgc
     2461 actttcggcg agatggacca ctcgagccac ttgtaggcgc aattcggatg ccgcgcgccg
     2521 gcagcgagca tcgtggtgtc cgcccaaccc gtcgcacctt cgaccggcac cgtagtggcg
     2581 accttcgcct tgttcatgag caacgtgttg gcctggaacg gccaggagcc cgaagcgacg
     2641 atgccttcgt tctggaagtc ctgcacctgg acgttggcgt cgtgccaata gcgctggatc
     2701 agcgggtgct ggccacgcag gacgtcgagc gccgccgcgt attgcgcctc gttgagctcg
     2761 tacggatcct tgatgccgag ttccggcttc ttggccttga cgtacaacgc ggcgtcggcg
     2821 atgtagatag ggccgtcata ggcctgaaca cggcccttgt ttgccttgcc atcgggaagc
     2881 ttctgcggct cgaacaccac cgaccaggac gtgggcggcg tcttgaaggt cacggtgttg
     2941 tacagcaaca cgttcggacc ccactggtaa ggcacgccgt agtgcttgcc gtcgacgtaa
     3001 tgccaagcgg cctgctgcag ccggggatcg atggtgttgt agctgggcac gcgtccgata
     3061 tcgatgggga tcacggtgcc gccgcggatc agccgcaacg acgcatcacc cgacgcggtg
     3121 accagatcgt agggcgcatt gcccggcggt ggccaggaag catcgcc
//
LOCUS       DASQJS010000462         4094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3453734, whole genome shotgun sequence.
ACCESSION   DASQJS010000462 DASQJS010000000
VERSION     DASQJS010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3453734"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VEW08_18205"
     CDS             <1..800
                     /locus_tag="VEW08_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42728.1"
                     /translation="NTDVDVNAKCATMGEKLTVRPGSEVVVAIVVRDPSGTNYSPYTF
                     ANPSLTQVGITQPLNKPVLDHIDVIGGSVTGYKVPGAADYSGEWPRTWINNPDLANVP
                     EGAKNTSAQIIRTFGQGTWKSSHGDKQYKTMVFRLRNLQNSQYLRLRGTNLPASVPFE
                     TDADGNPLADLWTNATGINPTLPGGADGVPANANLRIPCKPVGTNIPDTAVTYTGTAI
                     DGCPSHLPVVDGQKMSAYDVAAWADLWFYSNPVFIEVKGSSLVAGVK"
     gene            874..1734
                     /locus_tag="VEW08_18210"
     CDS             874..1734
                     /locus_tag="VEW08_18210"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYJ42729.1"
                     /translation="MSTTILVNETAPSVARPNFSPPTWMREPLLHFIVFGAMLFGIDH
                     VLASREGDPRTIVIDAEVDAHAVQVFKAARNREPNKEELYALRRVWLDNEVLYREGMA
                     LGLDKGDKAIRERVIFKALSMVDAGVKLPPLDETVLRNWFESHRQKYDEPARFDFEEA
                     VIAGDRSEAAARAFATALNSGAPGDVQAGLRVFTARPHDNIVQSYGAEFATALEAAPV
                     GEWQALPSREGLRVMRLKSMTPARPAVFEEVGTAVHQDWTDAVMSEQRSAAVSALAQK
                     YTVKVTGLKQ"
     gene            1731..2681
                     /locus_tag="VEW08_18215"
     CDS             1731..2681
                     /locus_tag="VEW08_18215"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HYJ42730.1"
                     /translation="MRLIARVVALLLLTIASSGAVAHEMTMAEMELRETRPGEFLWQW
                     GASGSRPIADELTPSWPESCRSEANVLRCGESGLSGVLSIEGVGERYSAALVKVFWRD
                     GQSRVYTLTERQPAVHLYGSADDKRGMGEIARAYLALGIEHILSGVDHLLFVAALLFL
                     VGFRRKLVWTITAFTLAHSLTLASAALGWLTLRSAPVEATIALSIVLVASEALNSRET
                     LSKRLPAAVAFLFGLVHGLGFAGALADIGLPQNHLATALLTFNIGVEIGQLLAVAAVW
                     AAWRVVRNWRLADWSRVPLLYGIGSLAAYWSWLRVAAVFS"
     gene            2690..3229
                     /locus_tag="VEW08_18220"
     CDS             2690..3229
                     /locus_tag="VEW08_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42731.1"
                     /translation="MDRRIRVLIGLSAVGLVAAGTVYFVTHPAESVARIPAVPDPSPR
                     VAPTGAMQPVVAPASVSTAASPGTVEQWIADTTSGDAAKRAAAIAALAEAPRATALPV
                     LGRILTDGEPQVDRPLALRSLRDLALNQGDDDGAIRDAVRHAIYHGDDFTRVEDVQEV
                     LDIIEESQLGQRLPSASQR"
     gene            complement(3199..3714)
                     /locus_tag="VEW08_18225"
     CDS             complement(3199..3714)
                     /locus_tag="VEW08_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42732.1"
                     /translation="MYRKLSLIALLALLPHQPAHADPIHAVADGAYWHHDSGWVFPEK
                     IGEYERVGIPQDVAGSPDAVGYYACVENGVRMTASVDVYLAKSAAAAELEGRPEGLLT
                     SEGAFPLSATPALAGTRLILEVGTKTPALEGLYFIHVGEWRVRIRITGSSADSMDTFV
                     RGQRWEALGNR"
     gene            3775..>4094
                     /locus_tag="VEW08_18230"
     CDS             3775..>4094
                     /locus_tag="VEW08_18230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ42733.1"
                     /translation="MIADIAVRLATLSEAADIAAMSRDYIERGLPWTWTEERVAQSIR
                     DPETNVVVAGEHGAIIGFGIMFYASDDAHLLLFAVRRAHQRRGVGSAILRWLEDVARE
                     AGATR"
BASE COUNT          728 a         1298 c         1332 g          736 t
ORIGIN      
        1 agaacaccga cgtcgatgtc aacgccaagt gcgccacgat gggtgagaaa ctcacggtgc
       61 gtccgggctc ggaagtggtg gtggcaattg tcgtgcgcga tccgtccggc acgaactact
      121 cgccttacac gtttgccaat ccgtccttga cgcaagtcgg gatcacgcag cccctcaaca
      181 agccggtgct cgatcacatc gacgtgatcg gcggttcggt tacaggctac aaagtgcccg
      241 gtgcggctga ctactccggc gagtggccgc ggacctggat caacaatccg gacctcgcca
      301 atgtccccga aggcgccaag aacaccagcg cgcaaatcat ccgcaccttc ggccagggaa
      361 cctggaaatc ttcgcacggc gacaagcagt acaagacaat ggtgttccga ctgcgcaatc
      421 tgcagaactc gcagtacctg cgcctgcgcg gcaccaacct gccggcctcg gtacctttcg
      481 agaccgatgc cgacggcaat ccgctggcgg acctgtggac caacgccacg ggaatcaatc
      541 cgacgctgcc gggtggcgcg gacggcgttc ccgcgaatgc caacttgcgc atcccctgca
      601 aaccggtggg cacgaacatc cccgacaccg ccgtgacata caccggtacg gcgatcgacg
      661 gatgcccgtc tcacctgccg gtcgtcgacg gccagaaaat gtcggcctac gacgtggcgg
      721 catgggcgga cctgtggttc tacagcaatc cggtattcat cgaggtgaaa ggctcttcgc
      781 tggtcgccgg cgtcaagtaa gtctctggtt aggtcgagtc ccggcgccgc gcggcaacgc
      841 gcggcgcttt cttgcatctg aagcgtaaga cacatgtcca ccacaatcct ggtcaacgag
      901 acggctcctt cggtcgcgcg cccgaatttt tctcctccca cgtggatgcg cgaaccgctg
      961 ctgcacttca tcgtgttcgg cgcgatgctg ttcggcatcg accacgtgct cgcatcgcgc
     1021 gagggtgacc cgcgcaccat cgtcatcgat gcggaagtgg atgctcacgc ggtgcaggtg
     1081 ttcaaagccg cgcgtaatcg tgagccgaac aaggaagagc tgtacgcact tcgcagggtg
     1141 tggctcgaca atgaagtgct gtaccgggag ggcatggcgc tcgggctcga caagggcgac
     1201 aaggcgatcc gcgaacgcgt gatcttcaag gcactgagca tggtggacgc aggagtgaag
     1261 ctgccgccgc tcgacgagac tgtgctgcgc aactggttcg aaagtcatcg gcagaaatat
     1321 gacgagcctg cgcgcttcga tttcgaggaa gcggtgatcg cgggcgatcg ttccgaagcg
     1381 gcggcgcggg cgtttgcgac cgccctcaac tccggtgcgc cgggcgacgt gcaagccggt
     1441 ttacgcgtgt ttacggcacg cccgcacgac aacatcgttc agagctacgg cgcggagttt
     1501 gccactgcgc tcgaagcggc gccggtaggt gagtggcagg cgctgcccag ccgcgaaggg
     1561 ttacgcgtga tgcgactcaa atcgatgacg cccgcgcggc ccgcggtgtt cgaagaggtt
     1621 ggcactgccg tgcaccagga ctggacggat gccgtgatgt ccgaacaacg cagcgcggca
     1681 gtgagtgcac tggcgcaaaa gtacaccgtg aaagtgacgg gcctgaagca atgagactga
     1741 tcgcgcgcgt ggtcgcgctg ttgttgctga cgatcgcgtc aagcggcgcc gtggcacatg
     1801 agatgaccat ggcggaaatg gagctgcgtg aaacacgccc cggagaattc ctgtggcagt
     1861 ggggtgcgag cggcagccgc cccatcgccg acgagctcac gccctcgtgg ccggagtcct
     1921 gccgcagcga agccaatgtc ctgcgttgtg gcgaatccgg cctcagcggc gttttgtcga
     1981 tcgaaggcgt aggcgagcgt tattccgcgg cgctggtaaa agtgttctgg cgcgacggcc
     2041 agagccgggt ctacacgctc accgagcgcc agcccgccgt gcacttgtac gggtcggcgg
     2101 acgacaagcg cggcatggga gaaatcgcgc gcgcctatct cgcgctcggc atcgaacaca
     2161 tcctgagcgg cgtcgatcac ctgttgttcg ttgccgcgct gctgttcctg gtcggctttc
     2221 ggcgcaagct ggtgtggacc atcaccgcct tcacgctcgc gcacagtctc acgctggcca
     2281 gcgccgcgct tggctggctc acgctgcgtt cggctccggt ggaagcgacg atcgcgctgt
     2341 ccatcgtgct ggtggccagc gaagcgttga attcacgcga gacgctgtcg aagcgcctgc
     2401 cggccgcggt cgcgtttcta ttcggactcg tgcacgggct ggggttcgcg ggcgctctcg
     2461 ccgacatcgg cctgccgcag aatcacctgg ccacggcgct gctcactttc aatatcggcg
     2521 tcgagatcgg gcagttgctg gcggtggccg cggtctgggc cgcgtggcgc gtggttcgaa
     2581 actggcgcct tgcggattgg agccgcgtcc cgctgctcta cggcatcggt tcgcttgccg
     2641 cgtactggtc gtggttgcgg gtcgccgcgg tatttagtta ggacacgcca tggacagacg
     2701 catacgtgta ctcattggcc tgtcggcggt tggccttgtg gcagcgggga ctgtctactt
     2761 cgtgacgcac ccggcggaat ccgtggcgcg catcccggcc gtgcccgatc cttcaccacg
     2821 ggtcgcaccg accggcgcga tgcaaccggt agttgcccct gcatcggttt caaccgcggc
     2881 ttcgcccggc actgtcgagc aatggattgc ggatactacg agcggcgatg ccgcgaaacg
     2941 cgccgcggcc atcgcggcgc tggccgaagc gccgagagcc acggcgctac cggtgctggg
     3001 gcgaattctc accgacggtg aaccacaagt ggatcgtccg ctggcgctgc gctcgctgcg
     3061 cgatctcgcg ctgaaccagg gtgatgacga cggtgccatt cgcgatgcgg ttcgccatgc
     3121 gatttatcac ggcgacgact tcaccagggt cgaagacgtt caggaagtac tcgacatcat
     3181 cgaggagtct caactgggtc agcgattgcc gagtgcttcc cagcgctgac cgcgcacgaa
     3241 cgtatccatt gaatcggcgc tggaacccgt gatgcgaatc cggacgcgcc actcgccgac
     3301 atggatgaag tagagacctt ccaacgccgg tgttttggtg ccgacctcta aaatcagcct
     3361 cgtgccggcc agcgctggcg tcgcactcag cgggaatgcg ccctcggacg tcaatagacc
     3421 ctcgggccgg ccttcgagct ctgctgccgc tgcggacttc gccaggtaca catccacaga
     3481 agccgtcatg cgaacgccgt tctcgacgca agcgtagtac cccacggcgt ccggtgatcc
     3541 ggcgacgtcc tgcggaatcc cgacgcgctc atattcaccg atcttctcgg ggaataccca
     3601 gccactatcg tgatgccagt acgcgccgtc ggcgaccgca tggatggggt cggcatgcgc
     3661 tggctggtgc ggcagaagcg cgagcagcgc gatcagcgaa agtttccggt acatggggcg
     3721 cagcctaaag aagctgtgtt gcagattcat acctgcgcgt gtatctgtcg gcccatgatc
     3781 gccgacatcg ccgttcgtct cgccacgctg tccgaagccg cggacatcgc cgccatgtca
     3841 cgcgactaca tcgagcgggg attgccgtgg acctggacgg aagagcgcgt ggcgcagtcc
     3901 attcgcgacc ccgagaccaa cgtcgtggtg gccggcgagc acggcgccat catcggcttc
     3961 ggcatcatgt tctacgccag cgacgacgcg catctgctgt tgttcgccgt gcgccgcgct
     4021 catcagcgcc gtggcgtcgg cagcgccatc ctgcgctggc tcgaggatgt cgcacgcgag
     4081 gctggtgcga cgcg
//
LOCUS       DASQJS010000463         6605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2821131, whole genome shotgun sequence.
ACCESSION   DASQJS010000463 DASQJS010000000
VERSION     DASQJS010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2821131"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VEW08_18235"
     CDS             <1..239
                     /locus_tag="VEW08_18235"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HYJ42734.1"
                     /translation="MCMNETDTGEQNVRDLVGEVANTISGNARRDFGRNFVISTPTIC
                     DRNNAATAAATTGRSFVIPINWRSYAANVVVCLQ"
     gene            complement(258..1163)
                     /locus_tag="VEW08_18240"
     CDS             complement(258..1163)
                     /locus_tag="VEW08_18240"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYJ42735.1"
                     /translation="MTERIQPNAIEELFGDARPRYRQRLEQSLGSGVIMDRDAHVITN
                     HHVIATADLIRAQLADGRIANAKIVGRDPDTDLAVLQLDLKTNVPVMPLGHSDRLRVG
                     DIVLAIGNPVGLSQTVTQGIVSATGRGQLGVATFENFIQTDAPINVGNSGGALINADG
                     ELIGINTAVLAKNLGVEGIGFAIPVNLVRGVMSEILTKGRVVRGWIGIVPADIDQIEA
                     RRFNLAQPGVVVAKLYVGSPAATVGMEPRDIILTVNGAKVATAQDTLTRIANFKPGAK
                     IKITGIRGTAPFESEVTVGERPRNR"
     gene            1577..2200
                     /gene="petA"
                     /locus_tag="VEW08_18245"
     CDS             1577..2200
                     /gene="petA"
                     /locus_tag="VEW08_18245"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HYJ42736.1"
                     /translation="MSDHAADDTVPEVDESRRKFLIAATAGTGAVGAVLTAVPFVASW
                     MPSESARAAGLPTEVDLSKIEPGQMATFFWRKQQIYVVKRTPEMLASLSAHDPQLKDP
                     KSEDSEQPQYAKNDARALKPEVLVLIGTCTHLGCLPKTRFTPGDATVMANWPGGFFCP
                     CHGSKFDLAGRVFQGSPASVNLKIPPYSFEGDNKLVIGVDAPAKEVA"
     gene            2200..3444
                     /locus_tag="VEW08_18250"
     CDS             2200..3444
                     /locus_tag="VEW08_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009566409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HYJ42737.1"
                     /translation="MSATMDKSAGGFRAWLNKRLPVDQFMADQLTGYYAPKNFNIWYF
                     FGSLALLVFVMQIVTGIFVTMHFKAGETTAWNSVQYMVREVPYMWLIQPMHAVGASFF
                     FIVVYLHMARAFLYGSYKGPRELLWVIGMLIYLALMAEAFMGYVLPYGNMSYWGAQVI
                     INLFGTIPGIGPDLVEWIRGDYGIGDATLTRFFSLHVIALPFIIALLILLHLVALRQV
                     GSNNPDGIEIKENLGADGKPKDGIPFHPYYTLKDIVGVGLFLTAFAIVVFFVPTFGGL
                     FMEAPNFEPANPSSTPEHIAPVWYFTPYYAILRAVPDQKLGALLMALSVVAFLFLPWL
                     DRAKNKSIRYRGVASKILLGVFFVTFLMLMWLGLKPADGIYVLLARIFTTLYFLFFIL
                     LPFVSKADGNGPVPDRVTYHAH"
     gene            3434..4207
                     /locus_tag="VEW08_18255"
     CDS             3434..4207
                     /locus_tag="VEW08_18255"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HYJ42738.1"
                     /translation="MPIKFASVLGGLALLLGAGAVSANEGGHAGWQREADNEISNLPS
                     LQRGARNFISYCSGCHSLKFVRYSRLAEDLKISDSLRDTYLVKPGSKFSDYINASMPA
                     ADAQEWFGKPPPDLSLAARSRGSDWIFNFLTTFYADPNSRQTGVNNLQLPGTAMPHVL
                     ASVQGVQNAVWTAVEHKGEDGKAAVLPEFKAFEPGVVGSLNKEQYDEFARDIVNFLQY
                     ASDPTQVERQSMGIWVVLFLLMFTGIAYMLKKEYWKDVR"
     gene            4265..4885
                     /locus_tag="VEW08_18260"
     CDS             4265..4885
                     /locus_tag="VEW08_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017138266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HYJ42739.1"
                     /translation="MSNRRATMTLFSAPDDPWSHRTRIVIAEKAIEIEIVDVAPGKYP
                     EDLLDLNPYHTTPTLIDRDLVLYDSRVIMEYLDERFPHPPLMPVDPVSRARARLALYQ
                     IEHDWYDLARKMDAPGSKDMAQLRKVLRDNVLGSADLFKLKPFFLSDEFSMVDATIAP
                     ILWRLPRYEIDLPPQAQPILKYASTIFSRPAFRASLSENEREMRLA"
     gene            4882..5283
                     /locus_tag="VEW08_18265"
     CDS             4882..5283
                     /locus_tag="VEW08_18265"
                     /inference="COORDINATES: protein motif:HMM:NF008769.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HYJ42740.1"
                     /translation="MNDRARNSKRPYLIRAMHGWMMDNGETPHVIVDAEKPGVEVPRA
                     YVKDGKVVLNLSSTATQRLVMGNDWIEFEARFAGMVHHVRIPVSTVLGIYARETGEGM
                     VFSEQDLGPEPPTKPASPEDGSRRPQLKVVK"
     gene            complement(5284..5934)
                     /gene="can"
                     /locus_tag="VEW08_18270"
     CDS             complement(5284..5934)
                     /gene="can"
                     /locus_tag="VEW08_18270"
                     /inference="COORDINATES: protein motif:HMM:NF007756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HYJ42741.1"
                     /translation="MKTLDELFKNNREWAAGILRQDPEFFTKLSRQQSPTYLWIGCAD
                     SRVPANQIVGLLPGDIFVHRNIANMVVHTDLNCLSVMQFAVDILKVKHILVVGHYGCS
                     GVQTAMRRARVGLADNWLRHVQDVHEKHHHSLKTCASENVASDRLCELNVIEQVQNVC
                     QTSIARDAWERGQELHVHGWIYGIADGLARDLGTTISDFHEAAPVYEAALGKLRRS"
BASE COUNT         1185 a         2058 c         2056 g         1306 t
ORIGIN      
        1 tgatgtgcat gaacgaaacg gacaccggcg aacagaacgt gcgcgatctc gtgggcgaag
       61 tcgccaacac catttcgggc aatgcgcgcc gggatttcgg ccgcaacttc gtgatcagca
      121 cgccgaccat ctgcgaccgc aacaatgccg ccacggctgc cgcgacaacg ggccgctcgt
      181 tcgtcattcc gatcaactgg cgcagctacg ccgcgaacgt cgtggtttgc ctgcagtagt
      241 tagctggcgc aaccgcctta tcgattccga gggcgttcgc ccacggtcac ttcgctttcg
      301 aacggcgctg ttccccgtat tcccgtgatc ttgatcttcg cgcccggctt gaagttggcg
      361 atgcgcgtga gcgtgtcctg cgcggtggca accttcgctc cattcaccgt gaggatgatg
      421 tcgcgcggct ccatgcctac ggtcgcggcg gggctgccca cgtagagctt ggcgaccacc
      481 acacccggct gcgcaagatt gaatcgccgc gcctcgatct ggtcgatgtc ggccggcacg
      541 atgccgatcc agccgcgtac cacacggccc ttggtgagga tttcggacat cacgccgcgc
      601 accaggttta ccggaatggc gaacccgatg ccttcgactc ccagattctt ggcgaggacc
      661 gcggtgttga tcccgatcag ctctccatcg gcattgatca gcgcgcctcc ggaattgccg
      721 acgttgatgg gcgcgtcggt ctggatgaag ttttcgaagg tcgccacgcc caactgaccg
      781 cggccggtgg cgctcacgat cccctgcgtg accgtctgcg acaggcccac cgggttgccg
      841 atggcgagca cgatgtcgcc gacgcgcagc cggtccgaat ggcccagcgg catcaccggt
      901 acgttcgttt tcagatcaag ctggagcacg gccaggtccg tgtccggatc cctgccgacg
      961 atcttcgcgt tggcgatgcg tccgtcggcg agctgggcgc gaatcagatc ggcggtggcg
     1021 atcacgtgat ggttggtgat cacgtgcgcg tcgcgatcca tgatgacgcc cgagcccagg
     1081 ctctgttcca ggcgctgccg gtagcgaggc cgggcatcgc caaacaactc ttcgatggcg
     1141 ttcggctgta tgcgctcggt cacgagccgg gccgtatata tgttgaccac tgctggcgct
     1201 gcccgctcga ccgcttccgc gtatgagttc accgccctcg gggtttccgg agccggaggc
     1261 tgttcagcgg gcgctgtatt gacggccggg gcggggagag cgggctgcgg cgcgggtggg
     1321 ggtgccggcg agcgagcaat aagctccggg cgcaccatca cggcgacgaa ggcggcagca
     1381 agtccgatga ttgctgaaac ggaaatgaaa gtcagaaatc tcaggaatgg tcgggtcgac
     1441 atgagccgga taatcctcat cggcggaagg tcttagcgtt gatgcgcatg tgtataatgc
     1501 gccgcctccc ggggtctcgc agcggcgcat tttgcgcccg cccgcgccgg ggtgttattc
     1561 gatcctgagg tcaagaatga gcgatcacgc ggccgacgat accgtccccg aagtcgatga
     1621 gagtcggcgc aagtttctga tagcggccac cgccggtact ggtgcggtag gtgcggtgct
     1681 gaccgcggtg cccttcgtgg cttcgtggat gccgtcggaa agtgcccgcg ctgcgggttt
     1741 gcccaccgag gtcgatctgt cgaagatcga gccgggccag atggccactt tcttctggcg
     1801 caagcaacag atctacgtgg tcaagcgcac gcccgaaatg ctggcgtcgt tgtccgcgca
     1861 cgatccgcag ctcaaggatc cgaagtccga ggactcggag cagccgcaat acgccaagaa
     1921 cgacgcgcgc gcgctcaagc ccgaggtgct ggtgctcatc ggcacttgca cgcatctcgg
     1981 ttgcctgccg aagacgcgct tcacgccggg tgatgcaacc gtcatggcca actggccggg
     2041 tggattcttc tgcccctgcc acggttcgaa gttcgacctc gcggggcgtg tcttccaggg
     2101 ctcgccggcg tcggtgaatc tcaagattcc gccatacagt tttgaaggcg acaacaagct
     2161 cgtcatcggc gtcgatgcgc ccgcgaaaga ggtggcctga tgtccgccac gatggataag
     2221 agcgccgggg gtttccgcgc ctggctcaac aagcgcctgc cggtcgacca gttcatggcc
     2281 gaccagctga cgggctacta cgcgccgaag aatttcaaca tctggtactt cttcggttcg
     2341 ctggcgctgc tggtattcgt catgcagatc gtgacgggca tcttcgtcac gatgcacttc
     2401 aaggcgggtg agaccacggc ctggaattcc gtgcagtaca tggtgcgtga agtgccatac
     2461 atgtggctca tccagcccat gcacgccgtc ggcgcgtcgt tctttttcat cgtcgtgtac
     2521 ctgcacatgg cacgcgcgtt cctgtatgga agctacaagg gaccgcgcga gttgctgtgg
     2581 gtcatcggca tgctgatcta cctcgcgctc atggccgaag ccttcatggg ctacgtcctg
     2641 ccgtacggca acatgtcgta ctggggtgcg caggtcatca tcaacctgtt cggcaccatc
     2701 ccgggcatcg gtccggacct cgtggaatgg attcgcggcg attacggcat cggcgatgca
     2761 acgctgacgc ggttcttctc gctgcacgtc atcgctctgc cgttcatcat cgcgctgctc
     2821 atccttctgc acctggtcgc cctgcgccag gtgggttcga acaatccgga tggcatcgag
     2881 atcaaggaaa acctgggcgc agacggcaag ccgaaagacg gcatcccgtt ccatccttat
     2941 tacacgctca aggacatcgt cggcgttggc ctgttcctga ccgcgttcgc catcgtcgtg
     3001 ttcttcgtgc cgaccttcgg cggcctgttc atggaggcgc cgaacttcga gccggccaat
     3061 ccgtcgtcca cgcccgagca catcgcgccg gtgtggtact tcacgcctta ctacgcaatc
     3121 ctgcgcgcgg tccctgacca gaaactcggt gcattgctca tggcgctgtc ggtggtggcg
     3181 ttcctgttcc tgccgtggct cgaccgcgcc aagaacaagt cgattcgtta tcgtggcgtg
     3241 gcatccaaaa tcctgctcgg cgtcttcttc gtcaccttcc tgatgctcat gtggctcggc
     3301 ctcaagcccg ccgacggcat ctacgtgctg ctggcgcgca ttttcacgac gctgtacttc
     3361 ctattcttca ttctgcttcc gttcgtctcc aaggcagacg gcaacggccc ggttcccgat
     3421 cgagtgacct accatgccca ttaagtttgc aagtgtcctc ggcggtctcg cgctgttgtt
     3481 aggcgccggc gctgtttcgg ccaacgaagg cgggcacgcc ggctggcagc gcgaggcgga
     3541 caatgaaatt tcgaacctgc cgtcattgca gcgcggcgcg cgcaatttca tcagctattg
     3601 ctcgggatgc cattcgctga agttcgtgcg ttattcgcgc ctcgcggaag acctcaagat
     3661 ttccgattcg ctgcgcgaca cctacctggt gaagccgggc agcaagttct ccgactacat
     3721 caatgcctcg atgcccgcgg ccgatgcgca ggaatggttt ggcaagccgc cgccggacct
     3781 gtcactggcg gcgcgttccc gcggcagcga ctggatcttc aatttcctca ccacctttta
     3841 tgcggatccg aacagccgcc agaccggggt gaacaacctg cagctgccgg gcacggcgat
     3901 gccgcacgtg ctcgcgagcg tgcagggtgt gcagaatgcc gtctggaccg ctgtcgagca
     3961 caagggtgaa gacggcaagg cggcggtgtt gccggaattc aaggcgttcg agccgggtgt
     4021 ggttggtagc ctcaacaaag agcagtacga cgagttcgcg cgcgacatcg tgaacttcct
     4081 tcaatacgcc agcgatccca ctcaggtcga gcgccagagc atgggtatct gggtagtgtt
     4141 gttccttctc atgttcacgg gcatcgcgta catgttgaag aaggaatact ggaaggacgt
     4201 ccgctgagcc gccggacgcg ccttcgccgg aagattcaac gcgccgtggt gcgtggaggg
     4261 cggattgtct aaccgccgtg caacgatgac gctgttctcg gcgcccgatg acccgtggag
     4321 tcatcgcacg cgaatcgtga tcgcggaaaa ggcgatcgag atcgaaatcg tcgacgtcgc
     4381 gccggggaaa tatcccgaag acctgctcga cctcaatcct taccacacca cgccgaccct
     4441 gatcgatcgc gacctcgtgc tctacgattc acgcgtgatc atggaatatc tcgacgaacg
     4501 ttttccgcat ccgccgctga tgccggtgga tcccgtatcg cgcgcccgag cacgcctggc
     4561 gctctaccag atcgagcacg actggtatga cctggcccgc aagatggacg cgcccggctc
     4621 caaggacatg gcccagctgc gcaaggtcct gcgcgacaac gtgcttggca gcgccgatct
     4681 cttcaagctc aagccgtttt tcctgtcgga tgagttctcg atggtggatg cgaccattgc
     4741 acccatcctg tggcggctgc cacgatatga aatcgatctg ccaccgcagg cgcagcccat
     4801 cctgaagtac gccagcacca tcttttcgcg cccggcattt cgcgccagcc tgtcggaaaa
     4861 cgagcgggag atgcggctcg catgaacgat cgcgcgcgca attccaagcg cccgtatctg
     4921 atccgcgcca tgcacggctg gatgatggat aacggcgaga cgcctcacgt catcgtcgac
     4981 gcagagaagc ccggagtcga agtacctcgc gcctacgtca aggacggcaa ggtggtgctc
     5041 aatctctcgt ccaccgcaac acagcggctg gtcatgggca acgactggat cgagttcgaa
     5101 gcgcgtttcg ccggcatggt gcaccacgtg agaattcccg tgagcacggt gctggggatt
     5161 tacgcgaggg aaaccggcga gggcatggtg ttctcggagc aggacctcgg tcccgagccg
     5221 ccgaccaagc ctgcgtcgcc cgaagacggc agccgccgtc cgcaactcaa ggtcgtcaag
     5281 tagttagctt cgcctgagtt tcccgagagc cgcctcgtag accggcgcgg cttcgtggaa
     5341 gtccgaaatg gtggtgccca gatcgcgcgc caggccgtcg gcaatgccgt atatccagcc
     5401 atgcacatgc agctcctggc cgcgttccca ggcgtcgcgc gcgatgctgg tctggcagac
     5461 gttctgcacc tgttcgatga cattgagctc gcacagccgg tccgacgcga cgttctcgct
     5521 cgcacaggtc ttgaggctgt ggtgatgttt ctcgtgcacg tcctgcacgt gccgcagcca
     5581 gttgtcggcg agcccgaccc gcgcgcgccg catcgccgtc tgcacgccgc tgcagccata
     5641 gtgcccgacc acgagtatgt gcttgacctt caggatgtcg accgcgaact gcatgaccga
     5701 caggcagttg agatcggtat gcaccaccat gttggcaatg ttgcggtgga cgaagatgtc
     5761 gccgggcagc aggccgacga tctggttggc gggcacgcgg ctgtcagcgc agccgatcca
     5821 aaggtaggtg ggcgattgct ggcgggaaag cttggtgaaa aattcagggt cctgacgaag
     5881 gatgcctgcg gcccattcac ggttgttctt gaacagctca tcgagcgttt tcatgtggcc
     5941 gcgaccttaa acggctgcgc ctcgtttggc aaaataatcg ggacattcct cttttgcttg
     6001 gaaaagggga cggggctcgt atgtggatgg tcgagcgacg ggcggccctg catgcgatga
     6061 gactcgcttt tcgccgggcc cttcgttcag ggtggcatcg ccagtttgcg ggccgccgac
     6121 acacgcgaag acactctggc gtagtttgat ttgaagatgg cgaagagtcc tgaagacgcg
     6181 gagatcggct tcgtgcctag cgaggccagg tatcccggcc cgctcgctca tcgcatgcag
     6241 ggccacccgt cgctcgatcg tgcaccgacg cgtcgccgct ccgttcgcca gaaaaagagg
     6301 cgtgtaaccc cacgctgtgg gaagacctca agtccgtgcg cttcttttct taccaccgca
     6361 gcaggaaagc ggggcgcggt tcggtgacgt atgagcccca gcgagcagtc cgcgcacagg
     6421 agctgagaga gcgggccggg atacctggcc tcgttaggca cgaagccgcc tctccgcatc
     6481 atcaggactc tcgcttccgg tcctcacaac cgcacgaaag tgtcattgcg tgtggcggcg
     6541 gcccgcaatc cagcgatgcc accctgaacg aagggcccgg cgaaaagcga acgaaatcct
     6601 gtgcg
//
LOCUS       DASQJS010000464         6140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1874599, whole genome shotgun sequence.
ACCESSION   DASQJS010000464 DASQJS010000000
VERSION     DASQJS010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874599"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="VEW08_18275"
     CDS             <1..1782
                     /locus_tag="VEW08_18275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYJ42742.1"
                     /translation="KPITNDQKRGIRQHFWAFDNKHVLYIQDEGGDENFHVYAVDVVA
                     GTQKDLTPYKGVRAEIMGLSWKKPGVIAVGLNDRVPEWHDVYEVDLNTGTRVLVEKNT
                     QEFAGYNLDFDLKPRIASKNDADGTELLRRVGDKWVSMLRIGQPDSLTSGLIGIEFSG
                     KTALLQSSVGRDKAALERVDLATGKTTLIAESKLADLEDVWLEPKTFKPQAYIVNYLK
                     PEITALDPAIKKDVDFLTKELGDGFGVGNRTLDDMFWSVTTDDALAPASTYIYDRKAG
                     KVTKLFDQRPALSKAPLVPMQALELKARDGLTLVSYLSLPPGSDKNGDNVPDSPVPLV
                     LNVHGGPWARDGYGFDNEHQWLANRGYAVLAVNYRGSTGFGKGFINASNKEWAGKMHD
                     DLLDTIDWAVKQKITTKDKVAIYGGSYGGYATLVGVTFTPDTFACGVDIVGPSNLNTL
                     LGSIPPYWKAFFEEFATRVGDPRTEEGKKLLNDRSPLSRVDAIKKPLLIAQGANDPRV
                     KQAEADQIVKSMKDKNLPVTYVLYPDEGHGFARPMNRTSFYAVAEGFLAQCLGGRYEP
                     VGNDFKGATLKVLEGAQYVPGLVEALK"
     gene            1937..2641
                     /locus_tag="VEW08_18280"
     CDS             1937..2641
                     /locus_tag="VEW08_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42743.1"
                     /translation="MAAVVRNRFYAVVALALALVVFAGFARTYYFRAWFDVPPITVLL
                     HLHSIAFTAWFVLFIIQTRLIAAQNYRTHMQLGIAGVVLAVIVTILGFATAILSADAP
                     RVRPMGMNSQQFVLVPMVGIAYFAVCVALAISFRRRADLHKRFMMLAMIAVLGPPVAR
                     LIFVTQTGKYFAAIQLAVSAAFVAWCLVSDWRKYRIVHPVYAIGGAILLISMPLRAMI
                     ARTPQWESVGRWFAGM"
     gene            2928..4553
                     /locus_tag="VEW08_18285"
     CDS             2928..4553
                     /locus_tag="VEW08_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42744.1"
                     /translation="MKTLYFCVIAMAAGASSFAQTTVPAAPSLTVGAEIKGLRFDWEP
                     VAGASWYQLEFKANENADFVQQDDDFDASVTSTRFRFPLHLYFWTPARYRLAACNSAG
                     CTRSAEVPVSSLRRFAVGYFKPAQTVIGQQFAADTDLSGTGRDFVSAAPGDFIPTTSG
                     PRAGGAIYLFRFNGGVWRQRVRLFPTIPPFIEGSNEMKVAISADGNTVALGMPNYFHE
                     EFDEQSGEVFVFKFNGSNWIRTRIRAGSRGLFGRWVGLSDAGDTLAIAHGENSNPSLP
                     PKIAIYKLVDGAWQPVRDIGNRFGRTEFCNQGVLSGDGSTVAESCEEGPVGTTPARVY
                     IRTHSGTNWTTRVDLPLQMSVGSEFGYGHNGIAIDSTGNTVAAQIFAASGPNPQVGPS
                     EVHVFEREDGVYEKEAVLTPGAWRNNTQRGFFGQSISISGNGAVLAVGDPWDNGLGNG
                     PRAAPLNPGSSRTGAVYVYRYYDDEWWLYNMAKPNYHPDNAPYLTYGSDVELNANGIA
                     MIVGEGGESSDAEGIAGGWSNIRAPGSGAVWLY"
     gene            4620..5384
                     /locus_tag="VEW08_18290"
     CDS             4620..5384
                     /locus_tag="VEW08_18290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42745.1"
                     /translation="MIAQTPAWEHVGQWLAGMEKGIRLKVSSTFFSIFFASAAMAGGA
                     KQTSTLPDPVKLAEQVTTGSVDLETFDFAGAGYWQLPQADRSSWWQGVAAALRLGLPA
                     TGNECSAESQRQGASIAWLMLLLQAPDADEWTKITQLLRKQLTTLDAALAAAAPAAKP
                     NADPRVTELLARYARDQAVREVFIEKKWTEGMPPLAVNNWPPAFISRMTAIDCSNTAW
                     LKTQLKEIHWFDIPTYGVEADKAAWHLSTSAVPNSA"
     gene            5722..>6140
                     /locus_tag="VEW08_18295"
     CDS             5722..>6140
                     /locus_tag="VEW08_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HYJ42746.1"
                     /translation="MSAAAAPRELNTYRKYWASRFGTAPFLPMSRAEMDELGWDSCDV
                     IIVTGDAYVDHPSFGMAIMGRLLEAQGFRVGIIAQPDWQSAEPFKQLGKPNLFFGITA
                     GNMDSMVNRYTSDKRIRSDDAYTPGGVGGKRPDRSVVV"
BASE COUNT         1138 a         1953 c         1897 g         1152 t
ORIGIN      
        1 aagcccatca ccaacgatca gaagcgcggc atccgccagc acttctgggc gttcgacaac
       61 aagcacgtgt tgtacatcca ggacgagggt ggtgacgaaa acttccacgt ctacgccgtg
      121 gacgtcgtgg ccggcacgca aaaggacctc acgccttaca agggtgtgcg cgccgagatc
      181 atgggactgt cgtggaagaa gcccggcgtc atcgccgtgg gcctcaatga tcgcgtaccc
      241 gaatggcacg acgtgtacga agtggatctc aacaccggca ctcgcgtgct ggtcgagaag
      301 aacacacagg agttcgccgg ctacaacctc gacttcgatc tcaagccgcg catcgcctcg
      361 aagaacgacg ccgacggcac ggagctgctg cgccgcgtgg gcgacaagtg ggtgagcatg
      421 ctgcgcatcg gtcagccgga ttcgctgacc agcggcctca tcggcatcga gttctccggc
      481 aagacggcgc tgctgcaatc ttcggtgggc cgcgacaagg ccgcgctcga gcgcgtggat
      541 ctcgccaccg gcaagaccac gttgatcgcc gagagcaagt tggcggacct cgaagacgtc
      601 tggctcgagc ccaagacgtt caagccgcag gcgtacatcg tcaactacct gaagccggaa
      661 atcaccgcgc tcgatcccgc gatcaagaag gacgtggatt tcctcaccaa ggagctcggc
      721 gacggattcg gcgtcggcaa ccgcacgctg gacgacatgt tctggtcggt aaccaccgac
      781 gatgcgctgg ctcccgcgtc cacctacatc tatgaccgca aggccggcaa ggtaaccaag
      841 ctgttcgacc agcggccggc gctgagcaag gcgccgctgg tgcccatgca ggcgctcgag
      901 ctcaaggcgc gcgacggcct cacgctggtc tcctatctat cgctgccgcc gggctccgac
      961 aagaatggcg acaatgttcc ggattcgccg gtgccgctgg tgctcaacgt gcacggtggc
     1021 ccgtgggccc gtgatgggta cggcttcgac aacgagcacc agtggttggc caaccgtggt
     1081 tacgctgtgc tcgcggtgaa ctaccgtggc tccaccggct tcggcaaggg attcatcaac
     1141 gcgtccaaca aggagtgggc gggcaagatg cacgacgatc tgctggacac catcgactgg
     1201 gcggtcaagc agaagatcac caccaaggac aaggttgcga tctacggcgg ctcgtatggt
     1261 ggttatgcca cgctggtcgg cgtcacgttc acgcccgaca cgtttgcgtg cggcgtcgac
     1321 atcgtcggcc cgagcaacct caacaccttg ttaggctcca tcccgccgta ctggaaggcg
     1381 ttcttcgaag agttcgccac ccgcgtcggc gatccgcgta cggaagaggg caagaagctg
     1441 ctcaacgacc gttcgccgct gtcgcgagtg gatgccatca agaagccgct gctgattgcg
     1501 cagggtgcca acgacccgcg cgtgaagcag gccgaggccg accagatcgt gaagtccatg
     1561 aaggacaaga acctgcccgt cacctacgtg ctgtatccgg acgaaggcca cggcttcgcg
     1621 cggccgatga accgcacgtc gttctacgcc gttgccgaag gattcctggc gcagtgcctg
     1681 ggtggccgtt acgagcccgt cggcaatgac ttcaagggcg ccacgctcaa ggtgctcgag
     1741 ggtgcgcagt acgtgcccgg cctcgtcgag gccctgaagt aagaaacacg gtgccgggtg
     1801 gggaaagtgg ggagtcgacg cgactcccca ctttccccac ccggcacctt cttccaaacg
     1861 gcaagaagtc tcagcggcga cgaaatcttg cgccgggttg attgtcggcc gccgtcgccg
     1921 gcggtacgtt cgcgtcatgg ctgccgtcgt ccgcaaccgc ttctatgccg tcgtcgcgct
     1981 ggcgctggcg ctagtcgtgt tcgccggctt cgcgcgcacg tactacttcc gcgcctggtt
     2041 cgacgtgccg cccatcaccg tgctgctgca tctgcacagc atcgcgttca ccgcctggtt
     2101 tgtgctgttc atcatccaga cgcggctcat cgccgcgcag aactaccgta cgcacatgca
     2161 gctcggcata gcgggtgtgg tgctcgccgt gatcgtgacg attctcggtt tcgccaccgc
     2221 catcctgtcg gccgacgcgc cgcgcgtgcg tcccatggga atgaattccc agcagttcgt
     2281 gctcgtaccg atggtgggta tcgcctactt cgccgtctgc gtggcgctgg caatctcatt
     2341 tcgccgccgc gccgatcttc acaagcgctt catgatgctg gcgatgatcg ccgtgcttgg
     2401 accgcctgtc gcgcgcctga ttttcgtgac tcaaaccggc aaatatttcg ccgcgataca
     2461 actggccgtg tccgcggcct tcgtagcctg gtgcctcgtg agcgactgga ggaaataccg
     2521 tatcgtccat cctgtctacg cgatcggcgg cgccatcctg ctcatcagca tgccgttgcg
     2581 cgccatgatt gcgcggacgc cgcaatggga gagcgtcggg cggtggttcg cgggaatgtg
     2641 agctagctgc cgggtgggga gtggggagtg gctgccactc cccactcccc acccggcacc
     2701 ttccgttgcg tacagcctcg cggccgctgc gcgggattgc agtgactgag cgggcggtgg
     2761 cattcgcatc ttcctacagc cccaatcgtc tgctatccgt ccgcaaattt acctggtgtt
     2821 gcgcactcgt ctgacacgcg caactcatgt tccacgcttg gttcacgccc tggaatttgc
     2881 tctcctgttg acgcgcacta gtttgtgcgt tcattcaggg aagcaatatg aaaaccctct
     2941 atttctgcgt catcgcgatg gcggcgggag cgtcttcctt tgcccagacc accgtacccg
     3001 ctgcaccgag cctcacggtg ggcgcggaga tcaagggatt gcgcttcgac tgggaacccg
     3061 tcgctggcgc aagctggtac cagctcgagt tcaaggcgaa tgaaaacgcc gatttcgtcc
     3121 agcaagatga cgatttcgat gcgtcggtca cctcgacacg ctttcgtttc ccattgcacc
     3181 tgtatttctg gacgcctgcg cgttatcgcc tcgcggcctg caacagcgcg ggctgcacgc
     3241 gctcggcgga agtcccggtg tcatcgctgc gccgattcgc cgtcggctac ttcaaaccgg
     3301 cacagacggt gatcggacaa cagttcgccg cggatacgga tctttcaggc actggccggg
     3361 atttcgtcag cgcggcacca ggcgatttca ttcccaccac cagtggccca cgtgctggcg
     3421 gcgccatcta tctgtttcgg ttcaacggcg gcgtgtggag gcagcgtgtg cggctcttcc
     3481 ccacgatccc accgttcatc gaaggcagca acgagatgaa ggtggccatc agcgccgatg
     3541 gaaacaccgt cgcgctcggc atgccgaact acttccacga ggaattcgac gaacaaagcg
     3601 gcgaagtgtt cgtgttcaag ttcaatgggt ccaactggat tcgcacgcga atccgcgcgg
     3661 gctcgcgcgg cctgtttggc agatgggtgg ggctcagcga cgccggcgac acgctggcga
     3721 ttgcgcacgg agagaattca aacccgtcgt tgccgcccaa gatcgccatc tacaaactcg
     3781 tggacggcgc ctggcagccc gtgcgcgaca tcggaaacag attcggccgc acggagttct
     3841 gcaaccaggg ggtgctgagc ggcgatggct cgacggtcgc ggagtcctgc gaggagggtc
     3901 cggtgggaac gactcctgcg cgcgtttaca tacgcacgca ttcgggaacc aactggacca
     3961 cgcgtgtgga tctgccgttg cagatgtccg ttggcagcga gttcggatac gggcacaacg
     4021 ggatcgcgat cgactccacc ggcaacacgg tcgcggcgca aatctttgcg gcgagcggac
     4081 ctaatccaca ggtcgggcca tcggaagttc acgtgttcga gcgggaagac ggcgtctatg
     4141 agaaggaggc tgtgttgacg ccaggtgcct ggcggaacaa tacccagcgc ggcttttttg
     4201 gccagagcat ctcgatcagt ggcaacggtg cagtcctggc ggttggagat ccctgggata
     4261 acgggcttgg gaatgggccg cgtgcggcac cgttgaaccc cggttcgtcg cgtaccggcg
     4321 ccgtctatgt gtaccggtac tacgacgacg agtggtggtt gtacaacatg gccaagccta
     4381 actaccaccc ggacaatgcc ccctacctca cgtacggaag tgacgtggag ctcaatgcca
     4441 acggcatcgc aatgattgtg ggtgaaggcg gcgaaagcag cgatgccgag ggcatcgctg
     4501 gcggctggag caacatcagg gcccctggct ccggagcggt ctggttgtat tgaagaattc
     4561 gagcgcgccg ccggagctcc ggcggcgcca tcccgctcat ccgcgttcct gtgtggttct
     4621 tgatcgcgca gacgcccgcg tgggaacatg tcggccagtg gttagccggc atggaaaaag
     4681 gtatacgcct gaaagtctca tccacgttct tctctatctt cttcgcgtcg gccgccatgg
     4741 ctggcggcgc gaagcaaaca tccactcttc ctgatcccgt caagctggca gagcaggtga
     4801 cgaccggcag cgtcgacctg gaaaccttcg acttcgccgg cgccggatac tggcagcttc
     4861 cccaggcgga tcgctcttcc tggtggcaag gcgtcgccgc ggcacttcgc ctggggctgc
     4921 cggccaccgg caacgaatgt tccgcggagt cccagcgcca gggtgcgtcc atcgcctggc
     4981 tgatgctgct cctgcaagcg ccggacgcag atgagtggac taaaatcacg cagcttctgc
     5041 gcaagcagct gacaacactc gatgccgcgc tggcggcggc tgcacccgct gcgaaaccca
     5101 atgcggatcc ccgcgtcacg gagttgttag cccgctacgc gcgcgaccag gccgtgcgcg
     5161 aggtgttcat cgagaagaag tggaccgagg gcatgccgcc gctcgcagtc aacaactggc
     5221 cgcccgcctt catctcgcgc atgacggcaa tcgattgctc gaacaccgcg tggctcaaga
     5281 cacagctcaa ggagatccac tggttcgaca ttccgaccta cggcgtcgag gctgacaaag
     5341 ccgcctggca cctctcgacg agcgccgtgc ccaactcggc atgaagccca tcgccaagca
     5401 cgcgctggac gtgtggcgcg agtcatgtcc gaagtgacgt caaaacgggc ctgacctcgc
     5461 cccgatttca caatcggcgg ctccaacagc ggttttcggg cgttttctac gactcttctt
     5521 tgacttcttc gcccccgtcc ttacactgcc gcccatccga cctctggcag taagccgcct
     5581 caaaacggcc gaccctcgag gagctcctga aattcaggaa atggtgttag ccggcgtaag
     5641 ttccggtgcg atttcgaacg caatggcgat ctgccattca cgcgctcggg ctccaaccca
     5701 tttccgcaat gaggttgtca gatgtccgcc gccgccgctc ccagagaact caatacctac
     5761 cgcaagtact gggccagtcg tttcggcacc gcgccgttcc tgcccatgtc gcgcgcggag
     5821 atggacgagc tcggctggga ttcctgcgac gtcatcatcg tcaccggcga tgcgtacgtg
     5881 gatcacccca gcttcggcat ggccatcatg ggccggttgt tggaggcgca gggctttcga
     5941 gtgggcatca tcgcgcagcc tgattggcag agcgccgagc ccttcaagca gttaggcaag
     6001 ccgaatctct tcttcggcat aaccgccggg aacatggatt ccatggtgaa ccgttacacg
     6061 tcggacaagc ggattcgttc cgacgacgcg tacacacccg gcggcgtcgg cggcaagcgg
     6121 ccggatcgca gcgtcgtcgt
//
LOCUS       DASQJS010000465         5835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_936232, whole genome shotgun sequence.
ACCESSION   DASQJS010000465 DASQJS010000000
VERSION     DASQJS010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936232"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /gene="metK"
                     /locus_tag="VEW08_18300"
     CDS             complement(<1..899)
                     /gene="metK"
                     /locus_tag="VEW08_18300"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HYJ42747.1"
                     /translation="MSSSFHFTSESVSEGHPDKVSDQISDAVLDALLAQDKFSRVGAE
                     TLCNTGLVVLAGEITSSAVVDFQQVARDTIKKIGYDNTDYGIDYKGCAVLVAYDKQSP
                     DIAQGVDEGKGLNLEQGAGDQGMMFGYACTETEGLMPLPIYLSHRLVEKQAELRKNGK
                     LKWLRPDAKSQVTVKYVDGKPSEIDTIVLSTQHHPDVEHKHIEQAVIEEIIKPVLAGQ
                     TIAKNIKYLVNPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDR
                     SACYMARYVAKNIVAAGLAERCTV"
     gene            complement(921..1895)
                     /locus_tag="VEW08_18305"
     CDS             complement(921..1895)
                     /locus_tag="VEW08_18305"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HYJ42748.1"
                     /translation="MPGKDKSALICGSVAYDTIMVFPDRFRAHILPDRLHMLNVAFLV
                     PQMRREFGGCAGNIAYNLKLLGETGYPMGGIGRDFQPYRDWMHKVGMSEKYLVTIPEE
                     HCAQAFITTDLDSNQITAFHPGAMNHSHRIKVPANDGISIGVIAPDSREGMLQHAEQF
                     KAAGIPFIFDPGQAMPLFAGDDFKRFISISDWLALNDYEWALLQERTGWSVADVMKHV
                     KALIVTRGSEGSTIHADGRTYEIPCAKAREALDPTGCGDAYRAGLIHGILRGLDWETT
                     GRIASLMGAIKIAVRGTQNHTFTRAEFADQYASAFGAPHPYKELGAAS"
     gene            complement(2125..4011)
                     /locus_tag="VEW08_18310"
     CDS             complement(2125..4011)
                     /locus_tag="VEW08_18310"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HYJ42749.1"
                     /translation="MRFAFAAALLLGAAVATAANQSPLNPEFDARNWASIGRSVGDTH
                     YSPLTEINRATVGRLRLAWTLDLDVGNAQSTPLAVDGVLYVAAGYSMVYAVDALTGKV
                     LWKYDPGVTQVAGKKLRAGAGVRGLAYSTGRLFVGTHDGRLIALDAKKGAALWSTPTL
                     DPNDATFISGAPRVFKGKVAIGFGDSGTTPGRVSTFDVVSGQPSWSWSTQGGGGAVWN
                     AITYDPENNRVYVGTGNARGADAAKNVFACSVVALNADTGAMEWHYDSAPGDHLQCDG
                     STDITLATVAIAGAPRNVILYAPKDGSFHVLDRASGKAISVKKLGMGAHNHFAQSFSP
                     KTGLTYVPTTELTASNVGDAPVDAGKSALVAWDPVKQQPLWAVPTPGAFSGGVLSTAG
                     ELVFQGQADGYLTAYSAADGRRAWAFYTATAALGAPISFAIGKRQYISILNGPTQGTA
                     GSLGAISARFGWDSRAHPRRLLTFVLDGAGSLPPTPGPTFATPVDGGDLVVDEALAKE
                     GAELFTKCQWCHGAGAVAGGGAPDLRASLSPLVAPSFASVVRGGVEIKGMPKFEELSD
                     RELDALRHYIRARARKVTRPDGAAPPAPEVAAPVPPAPAQEDSAPKPPPGSLESTGTP
                     PPRT"
     gene            complement(4023..5105)
                     /locus_tag="VEW08_18315"
     CDS             complement(4023..5105)
                     /locus_tag="VEW08_18315"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HYJ42750.1"
                     /translation="MKSDSGRADARRTVRVVMQEYLRRDSFLPVLRLLFDLAFLGAAI
                     VGACVFEAWWAKGLMVVPAAIAIARLFVIGHDACHGSYTKYDWLNKIVGRIAFLPSLT
                     PFSLWDVGHNVAHHGFTNLKGRDQVWVPLSAEEWRAASKIRRGLERIYRSGFGQGLYY
                     TIEMWWKKLILPSKKHVGVRRAIFIWDNVLVLGVGAAWITALVLWAVYDNHSVTWTLL
                     CGFVLPFLTFNCIIGWVIYVQHTHPEVAWFDKRDEWQKRLGFITTTINVVVPKALGRI
                     LHDILEHGAHHVNTGVPLYRLRAAQAALKDAVPQLARVYVLNWKRYWATVRTCKLYDY
                     ANHQWLDFKGRVTAAPLFPEAAPAAG"
     gene            5364..>5835
                     /locus_tag="VEW08_18320"
     CDS             5364..>5835
                     /locus_tag="VEW08_18320"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HYJ42751.1"
                     /translation="MQDINARIAGRVRALRAESAMSLKALATRCNVSRSMISLVERGE
                     TSPTAVILEKIATGLGCTLASLFDDGNAPANPVSRGAERASWKDPQSGYVRRNISPPN
                     FPSPIRIVDVAFPAGARVSYESGARDSGVAQQIWVQQGAIEVTVGKVTHRLAEDD"
BASE COUNT         1075 a         1818 c         1881 g         1061 t
ORIGIN      
        1 accgtgcacc gctcagcgag gccggctgcg acgatgttct tcgccacgta gcgggccatg
       61 tagcaggcgg agcggtcaac tttcgtcgga tccttaccgg agaaggcgcc gccgccgtgc
      121 ggggccgcgc cgccgtacgt atcaacgatg atcttgcggc ccgtgagacc gcagtcgccc
      181 tgcggaccac cgatcacgaa gcgaccggtc gggttcacga ggtatttgat gttcttggcg
      241 atcgtctgcc cggcgagcac cggcttgatg atctcttcga tcacggcctg ctcgatgtgc
      301 ttgtgctcca cgtccgggtg atgctgggtc gagagcacga tcgtgtcgat ttccgacggc
      361 ttgccgtcga catacttcac cgtgacctgc gacttcgcgt ccgggcgcag ccacttgagt
      421 ttgccattct tgcgcagctc ggcctgcttc tcgaccaggc ggtgcgagag gtagataggc
      481 agcggcatga ggccttcggt ctcggtgcac gcgtagccga acatcatgcc ctggtcgccg
      541 gcgccctgtt cgaggttcag gcccttgcct tcatcgacgc cctgcgcgat gtcgggggac
      601 tgcttgtcgt acgccacgag cacggcgcaa cccttgtagt cgatgccgta gtcggtgttg
      661 tcgtaaccga tcttcttgat ggtgtcgcga gcgacctgct ggaagtcgac gaccgcgctg
      721 gacgtgatct cgcccgcgag taccacgaga ccggtgttgc acagcgtctc tgcgcccacg
      781 cgcgagaact tgtcctgcgc gagaagcgca tcgagcacgg cgtcggaaat ctggtccgaa
      841 accttgtccg ggtgaccttc tgaaaccgac tctgacgtga aatggaagct gctggacata
      901 cgatcctctt gctgattgcg ctaactggcg gcgcccaatt ctttataagg atggggtgcg
      961 ccgaaggctg acgcgtattg gtccgcgaat tccgccctgg tgaatgtgtg attctgcgtg
     1021 ccgcgcacgg cgatcttgat ggcgcccatg agcgacgcaa tacgccccgt ggtttcccag
     1081 tcgagaccgc gaagaatgcc gtgaatcagg ccggcgcgat aggcgtcgcc gcaacccgtc
     1141 ggatcgagcg cttcccgcgc cttggcgcag ggaatctcgt aagtgcgccc gtcggcatga
     1201 atggtggaac cctcgctgcc gcgcgtgacg atgagcgcct tcacgtgctt catgacgtcc
     1261 gcaacgctcc agccggtgcg ctcctgcagc agtgcccact catagtcgtt cagtgccagc
     1321 cagtccgaga tggaaatgaa gcgcttgaaa tcgtcgccgg caaacagcgg catggcctgg
     1381 cccggatcga agatgaacgg gatgccggca gccttgaact gttcggcgtg ctgcagcatg
     1441 ccttcacggc tgtccggagc gataacacca atcgatatac cgtcattcgc cggcaccttg
     1501 atgcggtgcg aatggttcat ggcgcccgga tggaacgcgg tgatctggtt ggaatccagg
     1561 tccgtcgtga tgaatgcctg cgcgcaatgc tcttcgggga tggtcacgag atatttctcg
     1621 gacatgccga ccttgtgcat ccagtcgcga taaggctgga agtcgcggcc gatgccgccc
     1681 atggggtagc cggtttcgcc gagaagcttc aggttgtacg cgatattgcc cgcgcatccg
     1741 ccgaactccc ggcgcatctg tggaaccaga aatgccacgt tcagcatgtg caacctgtcg
     1801 ggaaggatgt gcgcgcggaa acggtcgggg aacaccatga tggtgtcgta cgcgaccgat
     1861 ccacagatga gtgcgctctt gtctttaccc ggcatgccaa tgggttacgc gtgagaaatg
     1921 ggggcgcaaa gatactcgaa acatgtcgtg gttgctcggc cgcgatggag ggccagccga
     1981 aatcccgcgc gaccggccac ctggcaccgt aagtgtatga aagagaagaa ttatttctct
     2041 ccgagtccaa ccggatgtgc ggcggcatcc tgaacgccac tcatatctat ggctcccggg
     2101 cggtccaggc gttatggaaa acggtcaagt ccgcggtggt ggagtgccgg tggactcgag
     2161 cgatccggga ggtggcttgg gggcggagtc ttcctgagcc ggcgctggcg gcacgggcgc
     2221 cgcaacttcg ggagccggcg gcgccgcgcc gtcgggtcgc gtcacctttc gggcgcgggc
     2281 cctgatgtag tgccgcaagg catcgagctc acgatcgctg agttcttcga acttgggcat
     2341 tcccttgatc tcgactccac cgcgcaccac gctggcaaac gatggagcga cgagtggcga
     2401 gagcgacgca cgcaggtcgg gcgcaccgcc accagcaacc gcacccgcgc cgtgacacca
     2461 ctgacacttc gtgaagagtt ccgcgccctc tttcgccagc gcttcatcga cgacgagatc
     2521 gccgccgtcg acgggtgtcg cgaatgtcgg acccggcgtg ggcggcagcg aaccggcacc
     2581 atcgagtacg aacgtgagca ggcgccgcgg atgcgcgcgt gaatcccaac cgaatcgcgc
     2641 ggatatcgca ccgaggcttc cagccgttcc ctgcgtgggt ccgttgagta tcgagatgta
     2701 ctgacgcttt ccgatcgcga agctgatcgg cgcgcccagt gccgccgtgg cggtatagaa
     2761 cgcccaggcg cgacggccat ctgccgcgga atacgcggtg agataaccat ctgcctgtcc
     2821 ctgaaagacc agctcgccgg cagtcgagag cacgccgccg ctgaacgcgc ccggggtcgg
     2881 cacggcccac agcggctgct gcttgaccgg atcccacgca accagcgcgc tcttgccggc
     2941 atccaccggc gcatcaccga cgttcgatgc cgtcaactcc gtggtgggca cataggtgag
     3001 gccggttttc gggctgaacg attgcgcaaa atggttgtgc gcgcccatgc ccagcttctt
     3061 tacggagatc gccttgccgc tggcgcgatc gagcacgtgg aaggaaccgt ctttcggcgc
     3121 gtagaggatg acattgcgcg gcgcccctgc gatggcgacc gtggccagcg tgatgtcggt
     3181 ggaaccgtcg cattgcaggt gatcgcccgg cgcggaatcg tagtgccact ccatcgcgcc
     3241 ggtatcggcg ttgagcgcca ccacgctgca ggcgaaaaca ttcttcgcgg catcggcacc
     3301 acgcgcgtta ccggtcccta catacacgcg gttgttttcc gggtcgtacg taatggcgtt
     3361 ccagactgcg cccccgccgc cctgcgtcga ccagctccac gagggttggc cgctgacgac
     3421 atcgaatgtg ctcacgcggc cgggcgtcgt gcccgaatca ccgaagccga ttgcaacctt
     3481 gcccttgaag acgcgcggcg caccggaaat gaaggtggcg tcgttcggat cgagcgtggg
     3541 cgtgctccac aaggccgcgc ccttcttcgc gtcgagcgca atgaggcggc catcgtgcgt
     3601 gccgacgaac agccgtcccg tggagtacgc gaggccgcgc acgcctgcgc cggcgcgtaa
     3661 cttctttcca gcgacctggg tgacgcctgg atcgtacttc cacaggactt tgccggtcag
     3721 cgcatcgacg gcatagacca tgctgtaacc ggccgcgacg tagagcacgc cgtcgaccgc
     3781 cagtggcgtg gactgcgcgt tgcccacgtc gagatcgagc gtccacgcca ggcgcaggcg
     3841 cccgaccgtc gcgcgattga tttcagtgag cgggctgtag tgggtgtcgc cgacgctgcg
     3901 gccgatagac gcccagttac gggcgtcgaa ctcggggttc agcggagatt gatttgccgc
     3961 ggtcgccacc gcggcaccaa ggagcaatgc agcggcgaag gcgaaacgca agcgacgatg
     4021 aatcagccgg cggcaggggc cgcttcaggg aacagcggcg ccgccgtcac ccggcccttg
     4081 aaatcgagcc actggtgatt ggcgtagtcg tacaacttgc aggtgcgcac ggttgcccag
     4141 taacgcttcc agttgagtac gtagactcgc gcgagctgcg gaacggcatc cttcagcgcc
     4201 gcttgcgccg cgcgcaaccg atacagcggc acgccggtgt tcacgtggtg agcgccgtgc
     4261 tcgaggatgt cgtgcaggat gcggccgagg gccttcggca cgaccacgtt gatggtggtg
     4321 gtgatgaacc ccaggcgctt ctgccattcg tcacgcttgt cgaaccaggc gacttccggg
     4381 tgcgtgtgct gcacgtagat gacccagcca atgatgcagt tgaatgtcag gaagggcagg
     4441 acgaagccgc agagcagtgt ccaggtgacg gagtggttgt cgtagacggc ccagagaacc
     4501 agcgcggtga tccacgcggc gcccacgcct aacaccagga cgttgtccca gatgaaaatc
     4561 gcgcgacgca cgcccacgtg tttcttgctg gggagaatga gcttcttcca ccacatttcg
     4621 atcgtgtagt agaggccctg gccgaaaccg gagcgataga tgcgctcgag cccgcgacgg
     4681 attttcgaag ccgcccgcca ctcttcggcg gacagcggca cccagacctg gtcgcggccc
     4741 ttgaggttcg tgaacccgtg gtgagcgacg ttgtggccca catcccagag cgagaacgga
     4801 gtcagtgagg gcaggaaggc gatgcggccc acgatcttgt tgagccagtc gtacttggtg
     4861 tagctgccat ggcaggcgtc gtggccgatg acgaacaacc gcgcgatggc gatggcggcc
     4921 gggaccacca tgagtccttt cgcccaccag gcctcgaaca cacacgcacc gacgatggcg
     4981 gcaccgagga atgcgagatc gaacagcagg cgcagcacgg gaaggaatga atcgcggcgc
     5041 aggtattcct gcatcactac gcgaacggtg cgcctggcat cggcgcggcc agaatcggat
     5101 ttcaaaggag actggggggc ttcggtcata agttggccaa gactatacga aacaaacgct
     5161 tcaaccaacg agttccgcgt cattttggac ctacgtcgca caagaaaaac cggcattccg
     5221 ggccccggac aagcgaccgt cttacacgtt gtgtcgaagc gttgtggcgt cgacacgaat
     5281 cgcatttgca ccacacaccc tctgcgggtt tctcgcacgc cggaattgac tgggcgtcca
     5341 ctttactgga tgtttcgtcc actatgcagg atatcaacgc ccggatcgcc ggccgggtgc
     5401 gcgcactgcg tgccgaatcg gccatgagcc tcaaagcact cgccacccgt tgcaacgtca
     5461 gccgctcgat gatctcgctg gtggagcgcg gcgaaaccag cccgaccgcg gtaatcctcg
     5521 aaaagatagc caccggcctc ggatgcaccc tcgcctcgct gttcgatgac ggcaatgcgc
     5581 ccgcgaatcc ggtctcgcgc ggtgctgaac gtgcctcgtg gaaagatccg caatcgggat
     5641 acgtgcgccg gaacatctca ccaccgaact tcccctcgcc gattcgcatc gtcgacgtgg
     5701 cttttccggc cggcgcgcgc gtgtcctacg aatccggtgc tcgcgactcg ggcgtagcgc
     5761 agcagatctg ggtgcagcag ggcgcaattg aagtcaccgt cggcaaggtg acgcatcgac
     5821 tggccgagga tgact
//
LOCUS       DASQJS010000466        13342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_617966, whole genome shotgun sequence.
ACCESSION   DASQJS010000466 DASQJS010000000
VERSION     DASQJS010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13342
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617966"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..642)
                     /locus_tag="VEW08_18325"
     CDS             complement(118..642)
                     /locus_tag="VEW08_18325"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYJ42752.1"
                     /translation="MRLATALNECVLRPWVPADKASLVANANNHRVWRNLADVFPHPY
                     TDADADQWFRIANSPGRSINFAIEVDGVAVGGIGARAGEGIFVRTAQFGYWLGESFWG
                     RGIMTAAGRVMLEHLRQDARFARLEAPVFEWNPASMRVLDKIGFVREAVRRNSVTKDG
                     QLIDSVLYTYLIKP"
     gene            complement(697..930)
                     /locus_tag="VEW08_18330"
     CDS             complement(697..930)
                     /locus_tag="VEW08_18330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42753.1"
                     /translation="MSVAQGHGSAPCRFRGQHPTVARPASRISLVSAVGRTRAGVLCR
                     STVRGLRASSRPRLRDSRQRRPAARNSPPRRTS"
     gene            complement(974..3355)
                     /locus_tag="VEW08_18335"
     CDS             complement(974..3355)
                     /locus_tag="VEW08_18335"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYJ42754.1"
                     /translation="MSILGLICVLLALVATLLFVLFVFQRRDLRAISELSLTVQRAIS
                     GERLPQRIEIENESDTQHEVQALGVSINQLLLRASRTTGREKAGPKLFTELGERIHEA
                     VLVHREVILYANSQFASFVGVDRVELIDRRLSDLVAPEYADLVGENLRRRLSGAHGAE
                     RFEVEMVGLQGQVSLLELTTALIDFEGSPALLVTGVEVIPTKKLRALASGKLDEKEAA
                     ARAAMVAPPLPAPPPVPAPLQVFAMQSLGEAIVTTDIEGRLAYLNPAAERLLGVLRAQ
                     AVGRLLEDVVGLVDPNDRKQLSEPVREAIGGGNGNPHSLSRRAVLVGKPSGEERAIEL
                     AASPLKNESGELAGAVILMHDVTELRGLHRQMSYQATHDALTGLVNRREFERRLDEAI
                     EGARRGDAAHMLCYLDLDRFKIVNDTSGHLAGDSMLREVAKLLREAVRDSDTVARLGG
                     DEFGLLLVGCPLDKARQIADDVCRSIATYRFVWHDRVFNVGVSIGLIEIGREAGSVEQ
                     LLAAADSACYVAKKEGAGRVSVYSSRDEALARSTGEIEWLGKLQSAIKEERFALYYQP
                     IVSAYGNETDGPSMEVLLRMIDEVGAEIAPLEFVAAAERYRLMASVDRWVVQTTLVAL
                     SRNAFQLARDRSVAINISAQTLADPLFLEFVVECLDRTGVAPDQVCFEISESAVIGNM
                     EAARRFVGVLHGMGCKFTIDDFGSGVASFSSLKNLPLDYLKLDGSFMRNLARDSVSQT
                     MVTAMINLARTLNFKIIAEQVEDSAALDVARRMGVDFVQGYVIARPTKLALAA"
     gene            3559..4209
                     /locus_tag="VEW08_18340"
     CDS             3559..4209
                     /locus_tag="VEW08_18340"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HYJ42755.1"
                     /translation="MQIEFARQQMITQQVRAWEVLDERVLDAMRRTPREFFVPESYAE
                     LAFADTDIPLRAGQHMLAPKVVGRLLQGLDAVPGMRALVVGSGLGYVPACLSAMGASV
                     RAVEIHGELASQSRANLKRAGFGQVDVVTGDTFNLDIGKDYAVIAVCGALPLYDDRFA
                     RALAVGGKLFVVVGNELPQEALLVTRTAESAWSSTALFETAVDPLENARRPEAFQF"
     gene            4216..4536
                     /locus_tag="VEW08_18345"
     CDS             4216..4536
                     /locus_tag="VEW08_18345"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYJ42756.1"
                     /translation="MVREIPVEELKARRDRGENPLVIDVREDWELQLARIPDVVHVPM
                     SQIPARLGEFQRDAEIIVMCHAGGRSLRVAQFLANQGFTDVANLSGGISAWSEAVDAT
                     VPRY"
     gene            4624..6072
                     /locus_tag="VEW08_18350"
     CDS             4624..6072
                     /locus_tag="VEW08_18350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HYJ42757.1"
                     /translation="MRRLLALGLGCLASFNASSEDLLTIFDQAVVNDPTVREAEFTRK
                     ATREARPQAWAAYLPQINGSYNKGESEGTRLTTDAQVIPDVNNPGSFVLQPFADNSSP
                     SPEFSGWSVELRQSIFSWQGLVGLKQSARIAAQADADYGVAQQDLALRVSTRYFDVLA
                     ALDNVQAQQASLDAISRQLEQADKRFEVGLIAITDVQEARAARDTAAADLIAAKRQLA
                     TSQEFLREITDMQYAELSVPRGTMPLAVPEPADPQKWVEASMEQNLALTSSRLGAEIA
                     RDDVRTAFGGHLPTVDLVVGKSHNESDDIKIFPAEPGFPGGTANSSFDNDTDQSISLQ
                     VQVPIWSSGGTHSRVKQSSYRWQAAKQRVERVSRETERTARDAYLGVMSEISRVEALK
                     QALESSRTALKATEAGYDVGTRTAIDVLAARQQLVQAYTNYSRSRYDYMLNVLRLKQS
                     AGLLDRKALQDVNSWLEAPPPAAPPVVSPPAQ"
     gene            complement(6077..7336)
                     /gene="waaA"
                     /locus_tag="VEW08_18355"
     CDS             complement(6077..7336)
                     /gene="waaA"
                     /locus_tag="VEW08_18355"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF004388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HYJ42758.1"
                     /translation="MRFLYICIAYLISPFWIGALALRGFRDRIHWRGFSQRFGFGAKV
                     SARCIWVHAVSVGEVQAAVPLIEALLKRFSSIPLVLTTVTPTGRARAEAQFGKRIDVR
                     YVPIDLPGSVWRFFQRVQPQLAIILETEIWPNLYHRCGRLGVPLVLASARISPRSVTS
                     YRRLVGLFRETLSHGIFIAAQSAEDAERFVSIGANPARTHVVGNIKFDFGYPPDMEAR
                     GHELRGLLGVHRPVWVAGSTHAKEEELLLDAHALIRQRFSNALLVLVPRHPPRFAEVV
                     ALLRDRSMPFVTRTSKNGVTSETQVFLLDTLGELPAFYAAGDVAFVGGSLVPIGGHNL
                     LEPAALSLPIVAGPHNFNSADIARMLIERGAVRIVHDAAQLSAVVGDLLTNPATRTVM
                     GASGRKAVDDNRGAVARLMQFLEPLLP"
     gene            complement(7344..8105)
                     /locus_tag="VEW08_18360"
     CDS             complement(7344..8105)
                     /locus_tag="VEW08_18360"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: protein motif:HMM:NF002475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HYJ42759.1"
                     /translation="MKRHWPVGAEVSEAAFEGGRMLYDTQRTSNFSAEFFEPEYWRRH
                     DSIEGSVLGRGTTYFIRTGDSSFVLRHYRRGGLMAKFSKDRYWWHGENETRAFAEWYL
                     TYHLHRAGLPVPTPIAARYQRRGLFYRADLITQRIENSEPLADRLLKGPLPLTQWIAV
                     GRCIRRFHDAGVCHADLNAHNILLTPAQVYLIDFDRGTLRKRGWWADTTLVRLYRSLE
                     KVTLLAAPESFTDEDWHSLLAGYRESAGLPQASMV"
     gene            8118..9173
                     /locus_tag="VEW08_18365"
     CDS             8118..9173
                     /locus_tag="VEW08_18365"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HYJ42760.1"
                     /translation="MDAPEKVCLLRLSAIGDTCHVVPLLRTLQSAWPRTRFTWVIGKL
                     EAKLMSLIPDVELITVDKGAGLSAFTRLRTEMQRRGSFDLLLHLQLSIRASATAACIP
                     APIKLGFDKPRARELQWLFTNRRIAERSREHVLDSFLGFADALGVPRQTPRWNIPLPP
                     AAIAYAQQLIPATTKGGQQTLVISACSSHRARNWIPERYAAVADYAVRKHGMRVILCG
                     GPATIEREMAAEIAKHATVPLIDQVGKDTLPQLLALLARATVLLTPDSGPAHMATMVE
                     TPVIGLYAATNPARSGPYESRRWCVNAYEDAARKFLGKPAAEIPWTRKIEVSGVMELI
                     EVPAVCSKLDELLALLK"
     gene            9170..9565
                     /locus_tag="VEW08_18370"
     CDS             9170..9565
                     /locus_tag="VEW08_18370"
                     /inference="COORDINATES: protein motif:HMM:NF039364.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HYJ42761.1"
                     /translation="MKDILVPVSPGEMLDKITILRIKAARMTDASKVANVRHELALLE
                     KTWKDSGAAAVDLGPEEANLTRVNEALWVIEDDIRDEERAGRFGEKFIELARAVYITN
                     DERAAIKKRINTLLGSSIVEEKSYKPYKP"
     gene            9604..10854
                     /locus_tag="VEW08_18375"
     CDS             9604..10854
                     /locus_tag="VEW08_18375"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYJ42762.1"
                     /translation="MTISRREVLSMTGAAALASLAPQRLFAADPSASLKSMTADVAPI
                     GPAEFLARIAKARALMAKLGIGALLIEPGASLVYFTGVQWWRSERLTAAVIPREGDIA
                     IVTPHFEEPSVRESLRVPAEVRTWNEDEDPLVTVAGILRDRKVTAPVAVEETVRFFAV
                     DALRRAMPRVDIVNGAPVVRGCRMVKSPAELALMQKAADITIAAYRHVAPRIKAGMSN
                     HDVGAMIDAAHAALGASPEFGLVLLGEASAYPHGSGKPQAVREGEVVLMDCGCSIEGY
                     HSDISRTFVFGEATAEQRKVWQQMRDGQQIAIETARVGVPAGSVDDAVRTQYVKWGYG
                     PGYKLPGLSHRTGHGIGLDGHEPVNLVHGEATALAPGMCFSNEPGIYLPGRFGIRLED
                     CFHMGPEKPIWFSTPPPSLDKPFG"
     gene            complement(10920..11117)
                     /locus_tag="VEW08_18380"
     CDS             complement(10920..11117)
                     /locus_tag="VEW08_18380"
                     /inference="COORDINATES: protein motif:HMM:NF021772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HYJ42763.1"
                     /translation="MPATKTLIQPNAQNEYKVDVGDLPLACPMPEMYLWNSHPRVYLA
                     IEETGWAKCPYCSAEYILSRG"
     gene            complement(11166..>13342)
                     /gene="glnE"
                     /locus_tag="VEW08_18385"
     CDS             complement(11166..>13342)
                     /gene="glnE"
                     /locus_tag="VEW08_18385"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HYJ42764.1"
                     /translation="FLDNYLQTHGRDWERYAWIKARAVTGTAAYKLIQAESVRPFVYR
                     RYLDFGVFEALREMKALIEKEVARRDLAEHVKLGPGGIREIEFVVQAFQLIRGGQDRR
                     MQTPSLLAVLPQLAGGKLLPARVAQELEAAYVFLRRLENRLQMLADAQAHTIPSDALT
                     RERIALAMGFGEWSACGAELDLHRARVTRAFQDVMFARNEAAPTEMPGVNGVLDAWVR
                     GAEGAQLAAALEARGFADATQAAALLTEFRGGGTLRRLDAPGRARLDTLMPRLLAAIV
                     DQRVDQGADLRLAEAPQIDVLRRILRVLEAIGSRSAYFALLNENAQVRRKLVELAARG
                     EFLAAQIASHPLLLDELLDDSAGARPTSRAELEAEVSSRLAQMAEDEPERQVEVLRQF
                     QRAAIFRVAMADLTGSIPLMRVSDYLTVIAEIIVEQAMRLAWAQMTAQFGVPQTNRRD
                     GPGRRAVNICAVGYGKLGGNELGYASDLDLVFLHDASAEPAETDAAKPVDNQVFFVRF
                     AQRALHLLTMHSAAGRLYEVDVRLRPSGKGGMLITRVGAFAEYQEKDAWTWEHQALLH
                     ARAVAGDAALRAEFERVRVDVLRRCVRRDTLRTEVRNMRERMRKELSRAKAGAGSFDI
                     KQDAGGTADIEFLAQYWALLHACTHPPVVMFADTIRQLESVASADLVPQETVDVLTLA
                     YRKYRERAHHLSLEKAEAVVPSGEFAEVRAAVTAIWNATMGD"
BASE COUNT         2400 a         4432 c         4249 g         2261 t
ORIGIN      
        1 ttccagcagc tggtgcagtt gcacttgttg gtcccctgcg ccagatcgat gtcggcctcg
       61 aacttcaccg caccgcaatg acaacttcca cggtaggttt tcaacattgc ccatccctca
      121 cggtttgatg agatacgtgt agagcacgct gtcgatgagc tgcccatcct tggtgacact
      181 gttgcgcctt actgcctcgc gcacaaatcc gattttatcc agcacgcgca tcgaagcagg
      241 attccactcg aacaccggcg cttcgaggcg cgcgaaccgc gcatcctgcc tgagatgctc
      301 gagcatcacg cggcctgctg ccgtcatgat gccgcggccc caaaacgact cacccagcca
      361 gtagccgaac tgcgccgtgc gcacgaagat gccctcgccc gcccgcgcgc ctatcccgcc
      421 gacggccacg ccgtcgacct cgattgcgaa attgatgctc cgcccgggac tgttcgcgat
      481 tcgaaaccac tggtcggcgt cggcgtcggt atacggatgt ggaaatacgt cggcgaggtt
      541 gcgccagacg cggtgattgt tggcgttggc gacaaggctg gccttgtcgg ccggcaccca
      601 gggccgcaac acacactcgt tcaaagccgt cgccaatcgc atgcggcccc cgtgaatgcc
      661 agggacgcaa tgatggccga gagtgggccg gttcgactag gaggttcttc gcggcggcga
      721 attgcgcgcg gccgggcggc gctggcgtga gtcccgcagg cgcggacggc tgctcgcccg
      781 caggccgcgt accgtggagc ggcacaacac gccggcacgc gtgcgcccca cggcgctcac
      841 cagggaaatg cgcgacgccg ggcgcgccac cgtcggatgt tgcccgcgaa agcggcaggg
      901 tgcgcttcca tggccttgtg caactgacat cttgagaact ccccgaactg caatcttgtt
      961 agttaaagaa ccgtcaggcc gcgagcgcga gctttgtcgg ccgcgcgatc acgtatccct
     1021 gcacgaagtc cacgcccatc ctgcgggcga cgtcgagcgc ggcgctgtct tccacctgct
     1081 cggcgatgat cttgaaattg agggtgcgcg cgaggttgat catcgcggtc accatggtct
     1141 ggctgacgga gtcccgcgcc aggttgcgca tgaatgaccc atcgagcttg aggtagtcga
     1201 gcggcaggtt cttcaggctg gagaacgacg cgacgccgga gccgaaatcg tcgatggtga
     1261 acttgcagcc catgccgtgc agcacgccga cgaatcggcg cgcggcttcc atgttgccga
     1321 tgaccgcgct ctccgagatc tcgaagcaca cctggtcggg cgcgacgccg gtgcggtcga
     1381 ggcactcgac cacgaactcg agaaacagcg gatcggcgag cgtctgcgcg gagatgttga
     1441 tggcgacgct gcgatcgcgc gcgagctgga acgcgttgcg cgacagcgcg acgagcgtgg
     1501 tctgcaccac ccaacggtcg accgacgcca tgaggcggta gcgttcggct gccgccacga
     1561 attcgagcgg cgcgatctcg gcgccgactt cgtcgatcat gcgcaacagc acctccatcg
     1621 acgggccgtc ggtctcgttg ccgtaggccg agacgatggg ctggtagtag agcgcgaagc
     1681 gttcttcctt gatggccgac tgcagtttcc ccagccactc gatttcgccc gtgctgcgcg
     1741 ccagcgcctc gtcgcgcgag gagtagacgg aaacgcggcc cgcgccttct ttcttggcga
     1801 cgtaacaggc cgaatccgcg gcggcgagca gctgctccac ggacccggct tcgcggccga
     1861 tctcgatgag gccgatcgac acgccgacgt tgaacacgcg gtcgtgccag acgaagcgat
     1921 acgtggcgat cgaacggcac acgtcatcgg cgatctgccg cgccttgtcg agcggacagc
     1981 cgaccagcaa caggccgaac tcgtcgccgc caaggcgcgc gaccgtatcc gagtcgcgta
     2041 ccgcttcgcg caacagcttc gcgacttcac gcagcatgct gtcaccggcg agatgaccgc
     2101 tggtgtcgtt gacgatcttg aatcgatcga gatcgaggta acacaacatg tgcgccgcgt
     2161 cgccacgtcg cgcgccttcg atcgcttcgt cgagtcggcg ctcgaactca cgccggttca
     2221 cgaggcccgt cagggcatcg tgcgttgcct ggtacgacat ctgccgatgc aggccgcgca
     2281 gctcggtgac gtcgtgcatg aggatgaccg cgccagcgag ctcaccgctt tcattcttca
     2341 atggcgatgc ggcgagctcg atggcgcgct cctcccccga gggcttgccg accagcacgg
     2401 cgcggcggct caaactgtgc gggttgccgt tgccgccgcc gattgcttcc ctgaccggct
     2461 cggaaagctg tttgcgatcg ttcgggtcga cgaggccgac gacgtcctcg agcagccgcc
     2521 cgacggcctg cgcgcggagc acgccgagca gcctctcggc tgccgggttc aggtacgcga
     2581 ggcgcccttc gatgtcggtg gtgacgatgg cttcgccgag cgactgcatg gcgaacacct
     2641 gcaacggcgc gggcacgggc ggcggtgccg gaagcggcgg cgccaccatc gcggcgcgcg
     2701 cggcggcctc tttctcgtcg agtttgccgc tggcgagcgc ccgcagtttc ttcgtcggaa
     2761 tcacttccac gccggtcacc agcagcgccg gggatccctc gaagtcgatc agcgcggtag
     2821 ttagttcgag caggctcacc tggccctgca acccgaccat ctccacctcg aagcgttcag
     2881 cgccgtgcgc cccggagagg cggcgacgca ggttttcacc gaccaggtcg gcgtattccg
     2941 gcgcgaccag gtccgacaag cggcggtcga tgagctcgac gcggtccacg ccgacgaagc
     3001 tcgcgaactg gctgttggcg tagaggatga cttcgcgatg cacgagcacc gcttcgtgga
     3061 tgcgctcacc cagctcggtg aacagtttgg gtcccgcctt ctcgcggcct gtggtgcgcg
     3121 acgcacgcag caacagctgg ttgatcgaaa caccgagcgc ctggacctcg tgttgtgtat
     3181 ccgattcgtt ttcgatttcg atgcgctgcg gcagccgctc gccgctgatg gcgcgctgaa
     3241 ccgtgagcga cagctcggag atcgcccgca aatcgcgccg ttggaacaca aacagcacga
     3301 agagcagcgt cgccacgagt gcgagcagca cacagatcaa cccaagaatc gacatcccga
     3361 aacgtaatcc tgttgttatt agtgcatctt cttgtgcgag gcatccgtgc cggtggccag
     3421 aatagcctta tcccgtcgag cactcattcg acattaacca taaaagtaag cgatgtgacg
     3481 actggttccg cgacgaaacg aagcgcttgc gaacgctgtt tcgggcagaa taagtagcat
     3541 cccgttgaag gagttgccat gcagatcgag ttcgcgcgcc aacagatgat cacgcagcag
     3601 gtacgcgcgt gggaggtgct cgacgaacgc gtgctcgatg ccatgcgtcg cacgccacgt
     3661 gaatttttcg tgcccgagag ctacgcggaa ctcgcatttg ccgacaccga catcccgctg
     3721 cgcgccgggc agcacatgct cgcgccgaaa gttgtcggac gactcctgca ggggctggat
     3781 gcggtgccgg gcatgcgtgc gctggtggtg ggctcgggcc tcggctacgt gccggcgtgc
     3841 ctttcggcca tgggcgccag cgtgcgcgcc gtcgaaattc acggcgaact cgcctcgcaa
     3901 agccgggcca acctgaagcg cgccggattc ggacaggtgg atgtcgtcac gggtgacact
     3961 ttcaacttgg atatcggcaa ggactatgcg gtcattgcgg tatgtggagc cctgcccttg
     4021 tacgacgatc gatttgctcg cgcgttggcg gtgggcggga agctgtttgt cgtggtcggc
     4081 aacgagcttc cgcaggaggc cctgctggtc acgcgcaccg ccgagagcgc ctggagcagc
     4141 actgcattgt tcgaaaccgc cgtggatccg ctggagaatg cccggcgccc cgaagccttc
     4201 cagttctaga gcgccatggt ccgcgagatc ccggtcgagg aactgaaggc acgccgcgat
     4261 cgcggggaaa acccgctggt gatcgacgtg cgcgaagact gggaactgca acttgcgcgt
     4321 attccggacg tcgtacatgt gccgatgagt cagataccgg cccggttagg ggagtttcag
     4381 cgcgatgcgg aaatcatcgt gatgtgccat gcgggcggca ggtcgctgcg ggtggctcaa
     4441 tttcttgcga accaaggatt tacggatgtc gccaacctct ccggcggaat atctgcgtgg
     4501 agtgaggccg tggatgcaac cgtaccccgg tactgaatcg tctaagtttt ctgaaatctg
     4561 cattgccttc gccccggtgg caggtgatat agtgaactac aacactaaag aggactctta
     4621 ttaatgcgcc gtttactcgc cttggggctg ggttgcctgg cctccttcaa cgcttcgagc
     4681 gaagacctgc tcacgatctt tgaccaggcc gtggtcaacg accccaccgt tcgcgaagcc
     4741 gagttcaccc gcaaggccac gcgcgaagcc cgcccgcaag cctgggccgc ttatctgccg
     4801 cagatcaacg gcagctacaa caaaggcgag agcgaaggca ctcggctcac caccgatgcg
     4861 caggttattc cggatgtcaa caatccgggc agcttcgtcc tgcagccgtt cgcggacaac
     4921 agttcgccct ctcctgaatt ttccggctgg agcgtcgagc tgcgccagag catcttttca
     4981 tggcaggggc tggtgggact caagcagtca gcgcgaattg ccgcccaggc ggatgcggat
     5041 tacggcgtag cccagcaaga cctggctttg cgcgtatcca cgcgctactt cgacgtgctc
     5101 gcggcgctcg acaacgtgca ggcgcagcag gcgtcgctcg atgccatttc gcgccagctc
     5161 gaacaggccg acaagcgttt cgaagttggc ctcatcgcca tcaccgacgt gcaggaagcg
     5221 cgcgccgcac gtgacaccgc cgcggcggat ctcatcgccg cgaaacgtca gctcgccacc
     5281 tcgcaggagt tcctgcgcga gatcaccgac atgcaatatg cggagctctc cgtgccgcgc
     5341 ggcaccatgc cgctcgccgt tcccgaaccg gcggatccgc agaaatgggt cgaagcctcg
     5401 atggagcaga acctcgcgct gacttcgagc cggctcggcg cggaaatcgc ccgcgatgac
     5461 gtgcgcaccg cgtttggcgg tcacctcccg actgtcgacc tggtggtcgg caagtcgcac
     5521 aacgaaagcg acgatatcaa gatatttcct gccgagccgg ggttccccgg tggcaccgct
     5581 aacagcagtt tcgacaacga caccgaccag tccatcagcc tgcaggtcca ggtgcccatc
     5641 tggtcgagcg gcggcacgca ctcgcgcgtg aagcagtcat cgtaccgctg gcaggccgcc
     5701 aagcagcgcg tcgagcgcgt gtcgcgcgaa accgagcgca ccgcgcgcga tgcctacctc
     5761 ggcgtcatgt cggaaatctc gcgcgtcgag gcgctcaagc aggcgctgga atccagccgc
     5821 accgcgctca aagccaccga agcgggttat gacgtgggca cccgaaccgc gatcgacgtg
     5881 ctggccgcgc gccagcagct ggtccaggcc tacacgaact actcgcgcag ccgctatgac
     5941 tacatgctca acgtgctgcg cctgaagcag tccgctggcc tgctcgatcg caaggcgctc
     6001 caggacgtga attcgtggct ggaagcgccg ccgccggctg cacctccggt cgtctcgccg
     6061 cccgcgcagt aactagtcag ggcaacaagg gctccagaaa ctgcatcagg cgcgcgaccg
     6121 cgcccctgtt gtcgtccacc gccttcctgc cactcgcacc catcacggtg cgggtggcgg
     6181 gattggtcag cagatccccg accactgcgg acagctgcgc cgcgtcgtgc acgattcgca
     6241 ccgcgccgcg ctcgatgagc atccgcgcga tatccgcgga attgaagtta tgcggccccg
     6301 ccacgatcgg caggctgagc gccgcgggct cgagcaggtt gtggccgccg atgggtacga
     6361 ggctgcctcc cacgaatgcc acgtcacccg cggcataaaa cgccggcagt tctccgagcg
     6421 tgtccagcag gaacacctgc gtttcggaag tcacgccgtt cttgctggtg cgcgtgacga
     6481 acggcatcga gcgatcgcga agcagtgcca ccacttcggc gaagcgcggt ggatgccgcg
     6541 gcaccagcac cagcaacgcg ttggagaagc gctggcggat cagcgcgtgc gcgtcgagca
     6601 gcaactcttc ttctttcgcg tgcgtgctgc cggccaccca taccgggcga tgcacgccta
     6661 acaaaccgcg cagttcgtgg ccgcgggctt ccatgtcggg cggatagccg aagtcgaact
     6721 tgatattgcc caccacgtgg gtgcgtgccg ggttggcgcc tatcgacacg aatcgctcag
     6781 catcttccgc gctctgcgcg gcgatgaaga tgccgtgcga aagcgtctcg cgaaacaggc
     6841 ccaccagccg ccgataactc gtcaccgagc gcggcgagat gcgcgcgctg gccagcacca
     6901 gcggcacgcc gaggcgaccg cagcgatggt agaggttggg ccagatctcg gtctcgagaa
     6961 tgatcgccag ctgcggctgc acgcgttgaa agaagcgcca caccgagccc ggcaggtcga
     7021 tgggcacgta gcgcacgtcg atgcgcttgc cgaactgcgc ctcggcgcgc gcccggcccg
     7081 tgggcgtgac cgtggtcaac accagtggta tcgaggaaaa gcgcttgagc agcgcctcga
     7141 tgagcggcac cgccgcctgg acttcgccca ccgacaccgc gtggacccag atgcagcgcg
     7201 cggaaacctt cgctccaaat ccgaaccgct gcgagaaacc ccgccagtgg atgcgatcgc
     7261 gaaagccgcg cagcgccagc gcgcctatcc agaagggcga gatcagatag gcgatgcaga
     7321 tgtagaggaa acgcacgcgg tggtcagacc atggaagcct gcggcaatcc cgcggactcg
     7381 cgataaccgg ccagcagcga gtgccagtct tcgtcggtga aactttcggg cgccgcgagc
     7441 agggtcacct tctcgagcga gcgatagagc ctgaccagtg tcgtgtccgc ccaccagccc
     7501 cgcttgcgca gcgtgccgcg atcgaaatcg atgagataga cctgcgccgg cgtcagcagg
     7561 atgttgtgcg cgttcagatc cgcgtgacac acgccggcat cgtgaaagcg gcggatgcag
     7621 cggcccacgg cgatccactg cgtgagcggc agtggcccct tcagcagccg gtcggccagc
     7681 ggctcgctgt tctcgattcg ctgggtgatg agatcggcgc gatagaacag gcccctgcgc
     7741 tgataacgcg ccgcgatggg cgtgggcacg ggcagtcccg cgcgatgcag gtggtaggtc
     7801 aggtaccact cggcaaaggc acgggtttcg ttctcgccgt gccaccagta ccggtccttg
     7861 gaaaattttg ccatcagccc gccgcggcgg taatgccgca gcacgaagct gctgtcgccc
     7921 gtgcggatga agtacgtcgt tccacggcca agaacactgc cttcgatgga atcgtgtcgg
     7981 cgccagtatt ccggctcgaa gaattccgcc gagaaattgc tcgtgcgttg ggtgtcgtat
     8041 agcatccggc cgccctcgaa ggcggcttcg ctgacttctg ctcccaccgg ccagtggcgc
     8101 ttcattcact cacttccatg gatgcaccgg aaaaagtctg ccttctgaga ttgtcggcca
     8161 ttggcgatac ctgccacgtg gtcccgctgc tgcgcacgct gcaaagcgcc tggccgcgca
     8221 cgcgcttcac ctgggtgatt ggcaaactcg aagcgaaatt gatgagcctc attccggatg
     8281 tggagcttat caccgtggac aagggcgccg gtctctcggc gttcacccgg ctgcggaccg
     8341 agatgcagcg gcgcggctcg ttcgacctgc tgctgcacct ccagctctcg atacgcgcca
     8401 gcgccaccgc cgcctgcata cccgcaccta tcaagctggg attcgacaag ccgcgcgcgc
     8461 gtgaactgca gtggctgttc accaaccggc gcattgccga acgttcccgc gagcacgtgc
     8521 tggacagttt cctcgggttc gccgacgcgc tcggcgtgcc gcgccagact ccgcgctgga
     8581 acattccgtt gccgcccgcg gcgatcgcat atgcccagca actgattccc gctacgacga
     8641 aaggcgggca gcagacgctg gtgatcagcg cgtgttcgag ccatcgcgcc cgcaactgga
     8701 ttcccgagcg ctacgccgcc gtggccgact acgcagtgcg caaacacggg atgcgcgtga
     8761 tcctgtgcgg cggtcccgcc accatcgaac gcgagatggc ggccgaaatc gccaaacatg
     8821 caaccgtgcc gctcatcgac caggtgggca aggacacgct gccgcagctg ctggcgctgc
     8881 tggcgcgggc gaccgtactg ctgactcccg actccggccc cgcgcacatg gcgaccatgg
     8941 tggaaacgcc cgtgatcggg ctgtacgcgg ccactaaccc cgcgcgcagc ggtccgtatg
     9001 aatcgcgccg ctggtgtgtc aacgcttacg aagatgccgc acgcaaattc ctcggcaaac
     9061 cggccgccga gattccctgg acgcgcaaga tcgaagtgtc cggagtcatg gagctcatcg
     9121 aagtgccggc cgtctgcagc aagctcgatg agctgctagc cttgctcaaa tgaaagacat
     9181 actcgtcccc gtgagcccgg gtgaaatgct cgacaagatc accatcctgc gcatcaaggc
     9241 cgcgcgcatg actgacgcct cgaaggtggc gaacgtcagg cacgagctcg cgctgctcga
     9301 aaagacctgg aaagactccg gcgctgccgc ggtcgatctg ggacctgaag aagccaatct
     9361 cacgcgcgtc aacgaagccc tgtgggtcat cgaagatgat atccgcgacg aagaacgcgc
     9421 tggccgcttc ggggaaaagt tcatcgaact ggcgcgcgcc gtgtacatca ccaacgacga
     9481 acgcgccgcc atcaagaagc gcatcaacac cttgctgggt tcgagcatcg tcgaagagaa
     9541 gtcatacaag ccctacaagc cctgagcaag gtgccgggtg gggattccgg ggagaagtgt
     9601 cggatgacta tcagcaggcg tgaagttctg tcgatgacgg gcgccgccgc gctcgcctcg
     9661 cttgcaccgc aacggttgtt tgccgcggac ccgtcagcca gcctcaaatc catgaccgcc
     9721 gacgtggcgc ccatcgggcc ggccgaattt ctcgcgcgca tcgccaaagc ccgcgcgctc
     9781 atggccaagc tcggcatcgg cgcattgctc atcgagcccg gcgcgtcact ggtgtacttc
     9841 accggcgtgc agtggtggcg cagcgagcgg ctcacggccg ctgtcattcc gcgtgaaggc
     9901 gacatcgcca tcgtcacgcc ccacttcgaa gaaccttcgg tgcgcgaaag cctgcgcgtt
     9961 cccgccgaag tgcgcacctg gaacgaagac gaagatccac tggtcaccgt cgccggcatc
    10021 ctgcgcgacc gcaaagtcac agcgccggtg gccgtcgagg aaaccgtgcg cttctttgcc
    10081 gtggatgcac tgcgccgcgc gatgccgcgc gtcgacatcg tgaacggcgc gcccgtggtg
    10141 cgcggatgcc gcatggtgaa gtcgcccgcc gagctcgcgc tcatgcagaa ggctgccgac
    10201 atcaccatcg ccgcgtaccg gcacgtcgcg ccgcgcatca aggccggcat gtcgaaccac
    10261 gacgtcggtg ccatgatcga cgcggcgcat gccgctctcg gcgcctcgcc cgagttcggg
    10321 ctggtgttgc tgggcgaggc ttctgcctat ccacacggat ccgggaagcc tcaggcagtg
    10381 cgtgagggcg aggtggtgct catggactgc ggctgctcga tcgagggtta ccactccgac
    10441 atttcgcgca ccttcgtgtt cggcgaagcc actgccgagc agcgcaaggt ctggcagcag
    10501 atgcgcgacg gccagcagat cgccatcgaa accgctcgcg tgggcgtgcc cgcgggcagc
    10561 gtcgatgacg ccgtgcgcac gcagtacgtg aagtggggct acggccccgg ctacaaacta
    10621 ccggggctct cgcatcgcac cggccacggc atcgggctcg acggccacga gcccgtcaac
    10681 ctggtgcacg gcgaggccac cgcgctggcg cccggcatgt gtttctcgaa cgaaccggga
    10741 atctatctac cgggcaggtt cggcatccgg ttggaggact gtttccacat gggacccgaa
    10801 aagcccatct ggttctcgac tcctccgcca tcgctggaca aaccgttcgg ctgacaatct
    10861 cacggacgcg gtcgcttcga ccgcgttttc cgacgtgggg cgtgagcccc tgggtattgt
    10921 cagcccctgg aaagaatata ctcagcgctg caatacgggc acttcgccca gccagtctct
    10981 tcgatcgcca ggtacacacg cggatgcgag ttccagagat acatctccgg catcggacag
    11041 gcgagcggca gatcgcccac gtccactttg tattcgttct gtgcgttggg ctggatcagt
    11101 gtcttggtgg ctggcatggg cgcggattat agggacgaca ctctcacgcc gctatcgcgg
    11161 cgatcctagt cgcccatcgt cgcgttccag atcgcggtta ccgcggcgcg gacttcggcg
    11221 aattctcccg aggggacaac ggcttccgcc ttctcgagcg acaggtgatg agcgcgctcg
    11281 cgatattttc gatatgccag cgtcagcacg tccacggtct cctggggcac caggtctgcg
    11341 gaggcgacgg attcgagctg ccggatggtg tcggcgaaca tgaccaccgg cggatgcgtg
    11401 cacgcatgca gcagcgccca gtactgcgcc aggaattcga tgtcggcggt gccgccggcg
    11461 tcctgcttga tgtcgaacga gcctgccccc gccttcgcgc gcgaaagttc cttgcgcatg
    11521 cgttcgcgca tgttccgcac ctcggtgcgc agggtgtcgc gccgcacgca gcgccggagc
    11581 acatcgacgc gcacgcgttc gaactccgcg cgcaacgccg catcgccggc cacggcgcgc
    11641 gcatgcagca gcgcctggtg ctcccaggtc caggcatcct tctcctggta ctcggcgaat
    11701 gcgccgacgc gcgtgatcaa catgccaccc ttacccgatg gcctcaggcg cacgtcgact
    11761 tcgtagagcc gcccggccgc cgagtgcatc gtcagcagat gcagcgcgcg ctgcgcgaag
    11821 cggacgaaga acacctggtt gtccacgggc ttggcggcat ccgtctcggc aggctctgca
    11881 ctcgcgtcat gcaggaacac gaggtcgagg tcggacgcat acccgagctc gttgccgcct
    11941 aacttcccgt aaccgaccgc acagatgttg acggcgcggc ggcccggtcc atcgcgccgg
    12001 ttcgtctgcg gcacgccgaa ctgcgcggtc atctgcgccc aggcgagccg catggcctgc
    12061 tcgacgatga tctcggcaat gaccgtgaga tagtcactga cccgcatcag cggaatcgag
    12121 ccggtgaggt ccgccatggc gacgcggaaa atcgccgctc gctggaactg ccgcagcacc
    12181 tccacctgcc gttcgggttc gtcttcggcc atttgcgcga gccgcgaact cacttcggct
    12241 tcgagctcgg cgcgcgacgt gggccgtgcg ccagcgctgt cgtccagcaa ttcgtcgagc
    12301 agcagcgggt gcgaggcgat ctgcgccgcg aggaactcgc cgcgcgccgc cagctccacc
    12361 agcttgcggc gcacctgcgc attttcgttg agcagcgcga agtacgccga gcgagaaccg
    12421 atggcctcga gcactcgcag gatccgccgc agcacgtcga tctgcggcgc ctccgcgaga
    12481 cgcagatccg ccccctgatc aaccctctga tcaactatcg ccgccaacaa cctgggcatc
    12541 agcgtgtcca ggcgcgcgcg gccgggcgcg tcgagccggc gcagcgtgcc gccgccgcga
    12601 aattcggtga gcagcgccgc ggcctgggtc gcgtcggcaa agccacgggc ctcgagcgcg
    12661 gcggcgagtt gcgccccttc cgcaccacgc acccaggcgt cgaggacgcc gttgacgccg
    12721 ggcatctcgg taggcgcggc ttcgttgcgc gcgaacatga catcctggaa tgcacgcgtg
    12781 acgcgcgcgc ggtgcagatc gagttcggcg ccgcaggcgc tccactcacc gaaccccatc
    12841 gccagcgcga ttcgttcgcg cgtgagcgcg tccgacggaa tggtgtgcgc ctgggcgtcg
    12901 gccagcatct gcaggcggtt ctccagacgg cgcagaaaaa cgtacgccgc ctcgagttcc
    12961 tgcgcgacac gagcgggtag cagcttgcca ccggccagct gcggcaagac cgccagcaac
    13021 gacggcgtct gcatgcgccg gtcctgcccg ccgcgaatga gctggaaggc ctgcacgacg
    13081 aattcgattt cgcgaatgcc gccgggaccc agcttgacgt gctcggcgag atcacgccgc
    13141 gcgacttcct tttcgatcag cgccttcatc tcgcgcagag cctcgaacac gccgaaatcg
    13201 aggtatcgcc ggtagacgaa tggccgcacg ctctcggcct ggatcaactt gtaggccgcg
    13261 gtgcctgtga cggcgcgcgc cttgatccac gcataacgct cccagtcgcg gccatgcgtc
    13321 tgcagatagt tgtcgaggaa ca
//
LOCUS       DASQJS010000467        10619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3140998, whole genome shotgun sequence.
ACCESSION   DASQJS010000467 DASQJS010000000
VERSION     DASQJS010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10619
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3140998"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /locus_tag="VEW08_18390"
     CDS             <1..1770
                     /locus_tag="VEW08_18390"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HYJ42765.1"
                     /translation="LRRTQDLNALLTAETKVLWRKHGSGPMSAHIWAPEIHFIDGKWY
                     VYFTASRNDAIWEVRPYVLENSSADPFKGEWKELGRLETGWDSFSLDGTTFKHKGKRY
                     FVWTQRGRTPEEGRGTNIYIAQMDSPKSITGKVTMLTRPEFEWEKRKYDVNEGPAVLI
                     RNGRVFMTFSASAIDANYCMGLLTASADADLLDPASWKKSLEPVFKSSEANGQYGPGH
                     NSFTTTPDGKTDLLVYHAREYRDILGSELADPNRNTRAQVLRWKGDGTPDFGEPEADL
                     GKVAPRPLYRDPLFDGAADPVIVYTGSYGRWYMLYTNRRANVQTTKGVEWVHGTRIGI
                     AESVDAGVTWKYIGEADIELPSEFGGSNATHWAPDVVRDDMGLFHMFLTAVPGIFEDW
                     NHPRRIVHLTSKDLRTWRDARPVTLATDRAIDASVIRLPSGGWRMFYNNEKDNKAIWY
                     ADSPDLEVWTDRGKLITDQAGEGPKAFRWRGKWWLITDVWKGLAVYRSDDGLTWTRQA
                     ENLLEAPGRGVDDQVKGAHADVMVSGDRAWLFYFTHPGRKDTKADTYETRRSSIQVVE
                     LQPNGEWLKAERDAPTRIALQAK"
     gene            complement(1782..3254)
                     /locus_tag="VEW08_18395"
     CDS             complement(1782..3254)
                     /locus_tag="VEW08_18395"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYJ42766.1"
                     /translation="MNRRRFLRSSFAGAITLAHLDRRLHAAADPIAEIEAMTGNGMKT
                     VLDKSSLQNLKSSLRGALLLPGEAGYDEARRVIDRSIDKRPALIVQPTGAADVRTATD
                     FARAHSLLVAVKCGGHSGFRSTCDGGLQMDLSRLRGVRVDPAARIAHVSGGCLLGDVD
                     HESMSFGLATATGAVSHTGVGGLTLGAGFGRLARRLGLTIDNVRAVDIVTADGQLRHA
                     SAEENPDLYWAVRGGGGNFGVVTAFEFRLHPRQKQVVGGRLVFPMSRAREVFDAYLAF
                     SLRAPEDLYADFILGQRCAIDICWSGDASAVRKILAPLGSLGTPLEDSIKAIDYLAIQ
                     HERDRSDPLNRNEERRGVQFRTAFIHGFDARLAHALVAGDEEHPQRRTSIYIQHAGGA
                     IARVAPDATAFPHRSATHVLNVTVSWPLPGDDAVHARYLENYWTAFKPLTDGYYANLV
                     DEDPPVVEGNYRGNLARLQRAKNKYDPTNLFRLNANIRPA"
     gene            3620..5233
                     /locus_tag="VEW08_18400"
     CDS             3620..5233
                     /locus_tag="VEW08_18400"
                     /inference="COORDINATES: protein motif:HMM:NF021041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HYJ42767.1"
                     /translation="MIGISAVRAGLRTGLCLFAASCIVSVPPVFAQAQPAQKDVEAGM
                     KRATQFMVDKVSYRGGFLWNYLPDMSRRWGEMEARETMIWLQPPGTSSMGHLFLDAYH
                     ATGDEYYYRAAEQVASALIWGQHPSGGWNYVIDFAGDRSLRDWYATVGKNAWRLEEFQ
                     HYYGNATFDDQTTTDAATFLLRLYVEKLDARYKAPVDRAIQFVLDSQYPIGAWPQRYP
                     LNHEFSKKGLADYSSYYTFNDDVAWENVSFLIQCYQVLGDQRLLDPITRGMNFFILAQ
                     QGAPNPGWALQYTPDLKPAAARTYEPLALATHTTASNLEHLITFYDLTGDTRYLARVA
                     EGIDWLEKVKLAKPDAGGRTHPTYIELQTNRPIYVHRRGSNIVNGEYYADYNPERPIG
                     HYGQTRKLNTAALRKHYEEALKADVKELAKNSPLAAGAGIVELPRFFADRYAPPADKD
                     LPGQVRSSLAALNKTGYWPAPLPQTSHPYKGMGGKEEVPGDFATTHVGDDTDTSPYTD
                     TAKTTSISTQEYLRNMNILIQWRAQAAQR"
     gene            complement(5240..6376)
                     /locus_tag="VEW08_18405"
     CDS             complement(5240..6376)
                     /locus_tag="VEW08_18405"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYJ42768.1"
                     /translation="MSEHDFDGSGYFLYHSIGMFPGKAERIAAALSDLGSLWGTPNDA
                     QWQQSLDIRDQFLRRWSDFIGAPTTSMTTAENVTTALCSIIGSLPHRHLEGRKLLIAA
                     DCFPSLHFLLSGMARRRGFVLHTVPMRAGEAWVRDEDFMAAWGPDVGVALLTWVTSTA
                     SHRCDLRALVEHGRRRGSLVGVDITQGVGIVPFDVTAVNADFVVGSTLKWLCGVAGAG
                     VLQVRESLLRECTPELRGWFSQENPFAWALDSFAYAGDARRFDHGTPSIFAAAACLPA
                     LDWHASKELQLLAGHTHGLCETAIEGCAGLGLELVSPASADDRGGSVMFRLPAGTDSV
                     AVVGSLREQNLFIDCRGDILRISPGNVTTAAGIERLLRALRTLL"
     gene            6412..7587
                     /locus_tag="VEW08_18410"
     CDS             6412..7587
                     /locus_tag="VEW08_18410"
                     /inference="COORDINATES: protein motif:HMM:NF020505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monodechloroaminopyrrolnitrin synthase PrnB
                     family protein"
                     /protein_id="HYJ42769.1"
                     /translation="MSNHSEAFDHWIRSSFMELNTELENLYFAQDDRSDVTRAGQNLK
                     QQLREEGHVHVVALLAEGDTGDGFANAFGVLGNVGLYLAALRRHELTNPSREEKSPFP
                     EASSLAMHVGAALGMAPRFSTAHIAHLNFAHAGVRKSFTSLPDEILFIDENTRGILLL
                     QLAADALTKVVPLGISSPIVDVPFAAAKHALAGVVESNDRLFARLDVERFFFSVRPYY
                     KPYRVGRFEYRGANAGDFSGINELDLLLGLCRANDPYYAQLLVDKMQFMPPADQKRLR
                     ECMRHTSFLDELLALAGKHAREAWFQRNARAYLELCGLYGRIAAQHHDQLVKRFIENP
                     AARLGAAHLADITASGPPLPVLVRSLEVLRDLRLAANRSDIATRHADFEQLRRVLEP"
     gene            complement(7615..9144)
                     /locus_tag="VEW08_18415"
     CDS             complement(7615..9144)
                     /locus_tag="VEW08_18415"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYJ42770.1"
                     /translation="MSTNSGRWTTSVGASLLAAMATQSFSADKPLGPSDVYGPRIAAT
                     ATTAAVINPPRRSSSTADTLRYWNQIAIDASGVDHTPVAAGENRVFGEQLGPGRSARA
                     IAIVHIAVFDAVNAITGGYRSYTGLARETRPASIDAAIATAARNTLVKMFPSQTANFN
                     SLYVRDLARVPASQDRQRLVGVAVGQRAAARILALRGDDGWAGTDPRMDTEWITSDAA
                     GHWRQDPISQAPIALGAHWGKVKPFVMRSPSQFRVPPPPAMTSAAYTAAYDEVKRLGG
                     DGVNTPTERSADQTHIGIFWAYDGTPSLCAPPRLYNQIATEISKNNTSVIQLARLYAL
                     LNVAMADAGIAGWESKYFYDFWRPVTGIRESDSGTGPSGTGDSNPATIGDAGFFPLGA
                     PASNLTGPNFTPPFPAYPSGHATFGAALFEMLRNVYGTDRVSFTFTSDEFNGQTVGND
                     GQVRPLLPRSFTRLSQAEEENGQSRIYLGIHWAFDKTAGIQQGRRIADYVFANAFQRV
                     N"
     gene            9298..10206
                     /locus_tag="VEW08_18420"
     CDS             9298..10206
                     /locus_tag="VEW08_18420"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYJ42771.1"
                     /translation="MTNRRQFLGAALFASAVFTSLAQGASKTGMLRRRIPSSGELIPA
                     IGMGTSGSFQIPAGSPEYDALKEVLKRFFEGGATLIDTAPTYGNAEDNLGPLLSEGGY
                     RKKAFIATKLSGVTGREAGLEQFNNTLRRLKTDKVELLQVHNLRDWKTQLEVARELKK
                     QGKVKYVGVTHYTDSAHDEIADIVQQSKPDFLQINHSVTNRGIEKRVLPVAKELGVAV
                     LTNRNFNDGALFGQVQGKPLPGWAADAGVTSWAQMFLKYSLSHEAVTVVIPATGKPER
                     QSDNLKAGEGPLLTAAQQKELIALFA"
     gene            10309..>10619
                     /locus_tag="VEW08_18425"
     CDS             10309..>10619
                     /locus_tag="VEW08_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42772.1"
                     /translation="MVDFSSRLVAAVVVATCVVAPAAADSSHWKRHKKRAPLELSVLG
                     TYSTGLGEASSEIAAYDPQSRRAFVTNAAARSVDILDVRNSSAPTRIERIDVTALGTP
                     N"
BASE COUNT         2087 a         3368 c         3295 g         1869 t
ORIGIN      
        1 ctgcgccgca cgcaggacct gaacgcattg ctcaccgcgg aaacgaaagt gttgtggcgc
       61 aagcacggat ccggaccgat gagcgcgcac atctgggcgc ccgaaatcca cttcatcgat
      121 ggcaagtggt acgtctattt caccgccagc cgcaacgatg cgatctggga agtccgtcct
      181 tacgtgctgg aaaattcttc ggcggatccc ttcaagggag agtggaagga gctcggccga
      241 ctcgagacgg gctgggacag cttttcgctc gatggaacca cgttcaaaca caaaggcaaa
      301 cgttacttcg tgtggacgca gcgcggccgg acaccggaag agggccgcgg caccaacatc
      361 tacatcgcgc agatggattc gccgaagtcg atcaccggca aggtcacgat gctgacccgt
      421 ccggaattcg agtgggaaaa gcggaagtac gacgtgaacg agggcccggc cgtgctcatc
      481 cgcaatggcc gcgtgttcat gacgttctcg gcgagcgcca tcgacgccaa ctactgcatg
      541 ggactgctga cggcatcagc cgatgcggac ctgctcgacc ccgcctcatg gaaaaagtcc
      601 ctcgaaccgg tattcaagag cagcgaggcc aacgggcaat acggcccggg ccacaacagc
      661 ttcacgacca cacccgacgg caagaccgac cttctcgttt atcacgcgcg cgaataccgc
      721 gacattctgg gctcggagct cgccgatccg aaccgcaaca cgcgcgcgca ggtgctgcgc
      781 tggaagggcg atggcacccc cgactttggc gaacccgaag ccgatctcgg caaggttgcg
      841 cccaggccgc tgtatcgcga tccgctcttt gacggcgctg ccgatccggt catcgtgtac
      901 acgggttcct acggccgctg gtacatgctc tacaccaacc gccgcgccaa tgttcagacg
      961 acgaagggcg tggagtgggt ccacggcacg cgcatcggca ttgccgaatc agtcgatgcc
     1021 ggcgtgacat ggaaatatat cggcgaggca gacatcgaat tgccgtccga atttggcggc
     1081 agcaatgcga ctcactgggc gccggacgtg gtgcgcgacg acatgggcct gttccacatg
     1141 ttcctcacgg cggttccggg gattttcgag gactggaatc atccgcggcg catcgtccat
     1201 ctcaccagca aggacctgcg tacctggcgc gatgcccggc ccgtcacgct cgcgacggat
     1261 cgcgcgatcg acgcctcggt cattcggctc ccatccggcg gctggcgcat gttctataac
     1321 aatgagaagg acaacaaggc gatctggtac gccgacagcc cggacctcga agtctggacc
     1381 gatcgcggca agttgatcac cgaccaggcc ggcgaaggac cgaaagcctt ccgctggcgg
     1441 ggcaagtggt ggctgatcac cgatgtatgg aaaggccttg cggtttatcg ttccgatgac
     1501 ggcctgacct ggacgcgcca ggctgagaat ctgctcgaag ctccaggcag aggcgtggac
     1561 gaccaggtga agggcgcgca cgcagacgtg atggtgagcg gcgaccgcgc gtggctgttc
     1621 tatttcacgc accctggccg caaggatacg aaggccgaca cgtatgagac tcggcgcagc
     1681 tcgatacaag tggtggagct gcagccgaat ggagaatggc tgaaagcgga acgcgacgcc
     1741 cccacgcgca ttgcgctgca ggccaagtga gttagctcaa ttcacgccgg cctgatgttc
     1801 gcattgagcc tgaaaagatt cgtaggatcg tatttgttct tggcccgctg caggcgcgca
     1861 agattgccgc gatagtttcc ttcgaccacg ggcgggtcct cgtcgaccag attcgcgtag
     1921 tagccatcgg tgagtggctt gaaggctgtc cagtaatttt ccaggtaacg agcatgcacc
     1981 gcatcgtcgc ccggaagtgg ccatgacacg gtcacattca ggacgtgagt ggcactgcgg
     2041 tgcgggaagg cagtcgcgtc cggcgcgacg cgggcgatcg cgccgccggc atgctggatg
     2101 tagattgagg tgcggcgttg cgggtgttct tcatctcccg cgaccaatgc atgcgcgagt
     2161 ctcgcgtcaa aaccatgtat gaacgccgtc ctgaactgca ccccgcgccg ctcttcgttg
     2221 cggttgagcg gatcggagcg atcacgctcg tgctggatgg ccaggtagtc gatcgccttg
     2281 atgctgtcct cgagtggggt gccgaggctg cccaacggcg ccagtatttt cctgaccgcg
     2341 ctcgcgtcgc cggaccagca gatgtcgatg gcacagcgtt gtcccagtat gaagtccgcg
     2401 tacaggtctt cgggggcccg caggctgaat gccaggtatg catcgaacac ctcgcgcgcg
     2461 cggctcatcg gaaaaaccag ccggccgccg acgacctgtt tctgtctcgg atgcaggcgg
     2521 aattcaaagg ccgtcacgac gccgaaattg cctccacctc ctcgcaccgc ccagtagaga
     2581 tctggattct cctcggcgct cgcgtgacgc aactggccgt cggcagtcac gatatcgacg
     2641 gcgcgcacat tgtcgatcgt cagtcccagc cggcgcgcga gccggccgaa gcccgcaccg
     2701 agcgtcaagc cgccgacccc ggtgtgcgac acagccccgg tggcggttgc cagaccaaag
     2761 gacatcgatt cgtggtccac atcgccgagc aaacagccgc cggagacatg agcgatgcgt
     2821 gccgcggggt cgacgcgtac tccacgcaag cgcgacagat ccatctgcag accgccgtcg
     2881 cacgttgagc ggaatccact gtgcccgccg catttcaccg caacgagcag cgaatgggcc
     2941 cgcgcgaaat cggtcgccgt acgaacgtcc gctgcgccag tcggttgcac gatcaacgcc
     3001 gggcgtttgt cgatcgatcg atcgatgaca cgtcgggcct cgtcataacc ggcctcgccg
     3061 ggcagcaaca acgcaccacg caggctgctc ttcaggttct gaagcgagct cttgtcgagc
     3121 acggtcttca tgccgttgcc ggtcatcgcc tcgatctcgg caatggggtc cgccgccgca
     3181 tggagacgac gatcgagatg cgccagggtg atggccccgg cgaaagatga gcgcaggaat
     3241 cgtcgcctgt tcatgcagcc acgcaatcgc tgtgttgtta gagcgccgcc gccgagagca
     3301 taaccccggc cggcgccggc gtgttagcac accgacaaac caggatcatc gttcccgcca
     3361 cattgttgcg gcgcgccagc ggatctgcgg caggcctaac ttctcgcgca atgcccgaat
     3421 gtcgcgcgat gtccccggtt tgtcgccaca ggcactgaag tggacaagca cgatgaggac
     3481 aagtgctgtc gtgagacatc ctgacgcggg gactctgcga cctttgtctt aatgaataaa
     3541 tcttcatttg gatgactgcc cggccggaat cgccaatact cgcacccctg ctgctcatca
     3601 aagcctcgag gggtcgatca tgattggaat ttccgccgtg cgcgccggct tgcgcactgg
     3661 tctttgtttg ttcgccgcat cctgcatcgt ctctgtgccg ccggtcttcg cccaggcgca
     3721 gccggcgcag aaggacgtcg aagctggaat gaagcgcgcg acccagttca tggtggacaa
     3781 ggtgtcgtac cgcggtggtt ttctctggaa ctatctaccc gacatgtcgc gccgctgggg
     3841 cgagatggaa gcgcgcgaga ccatgatctg gctgcagccg ccgggcacgt cgagcatggg
     3901 ccacctcttc ctcgatgcgt atcacgcgac cggtgatgag tactactacc gcgcggccga
     3961 gcaggtcgca tccgcattga tctggggcca gcatccaagc ggcggctgga actatgtgat
     4021 cgacttcgcc ggagaccgaa gcctgcgcga ctggtatgcg accgtgggca agaacgcctg
     4081 gcgtctcgag gagttccagc attactacgg caacgccacg ttcgacgacc agaccaccac
     4141 ggacgcggca acattcctgt tgcggctgta cgtggaaaaa ctcgatgcgc gctacaaggc
     4201 gccggtcgat cgcgccattc aattcgtgct cgacagccag tatccgatcg gcgcctggcc
     4261 gcagcgatat ccgctgaacc acgagttttc caagaaggga ctggcggact actcttctta
     4321 ttacaccttc aacgacgacg tcgcctggga gaacgtctcg ttcctgatcc agtgctacca
     4381 ggtgctgggc gatcagcggc tgctcgatcc catcacgcgc ggcatgaact tcttcatcct
     4441 cgcgcagcag ggcgcgccaa atccgggctg ggcgttgcag tacacgcccg acctcaagcc
     4501 cgccgcggcg cgtacttatg aaccgctggc attggcgact cacaccacgg ccagcaacct
     4561 cgaacacctg atcacgttct acgacctcac gggcgacacg cgttatctcg cgcgcgttgc
     4621 tgagggaatc gactggctcg agaaagtgaa gctggcgaag cccgatgcag gtggcagaac
     4681 gcatcccaca tacatcgaac tgcagacgaa ccgcccgata tacgtgcatc gtcgcggctc
     4741 caacatcgtc aacggggagt actacgccga ctacaacccg gagcgcccca tcgggcacta
     4801 cggccagact cgcaagctca acaccgccgc gctacgcaag cactacgaag aagcgctcaa
     4861 ggcggatgtg aaggaactgg caaagaactc tccgctcgcg gctggcgcgg ggatcgtcga
     4921 gcttccgcgt ttctttgcgg atcgttatgc gccgcccgcg gacaaggatc ttccgggcca
     4981 ggtgcgcagc tctctggcgg cgctgaacaa gacgggttat tggcccgcgc cgttgccgca
     5041 gaccagtcat ccctacaagg gcatgggagg gaaagaagag gtgccgggag actttgcgac
     5101 cacgcacgtg ggcgatgaca ccgacacctc gccgtacacg gacacggcga agacgacgtc
     5161 gatctccacg caggaatatc tgcgcaacat gaacatcctg attcagtggc gcgcgcaggc
     5221 agcgcagcgc tgactcgcgt cagagcagcg ttcggagagc ccgcagcaaa cgctcgatac
     5281 cggccgccgt cgtcacgttg ccgggggata tccgcaggat atctccccgg cagtcgatga
     5341 acaggttctg ttcgcgcagg cttccaacga ccgccacgga atccgtaccc gcgggcaatc
     5401 gaaacatcac actgccgccg cggtcgtccg ccgacgccgg gctaaccagt tcgagaccga
     5461 gcccggcgca accttcaatc gccgtttcgc acaatccgtg ggtgtgcccc gccagcaatt
     5521 gcaactcctt cgacgcatgc cagtcgagcg cgggcagaca cgctgcagcc gcgaaaatcg
     5581 acggcgtgcc gtgatcgaag cgacgtgcgt cgccggcata cgcaaatgaa tcgagcgccc
     5641 aggcgaaagg attctcctgg ctgaaccagc cacgcagctc gggcgtgcat tcgcgaagca
     5701 gcgactcgcg aacctgcagc acgccggcgc ccgcgacgcc gcagagccac ttgagcgtcg
     5761 agccgaccac gaagtccgca ttcaccgccg tcacgtcgaa cggtacgatg cccacgccct
     5821 gcgtgatatc aacgcccacg agcgagccac gcctgcggcc atgttcgacg agagcgcgca
     5881 aatcgcagcg atgcgaggca gtggaagtta cccaagtcag cagggcgaca ccgacgtccg
     5941 gcccccaggc cgccatgaaa tcttcgtccc gcacccaggc ttcgcccgcg cgcatcggta
     6001 cggtgtgcag gacgaacccg cgccgccggg ccatgcctga cagcagaaaa tgcaggctcg
     6061 ggaagcaatc cgccgcaatg agcagctttc ggccctcgag atgccggtgg ggcagagagc
     6121 cgatgatgga gcacagcgcc gtcgtgacat tttccgccgt ggtcatcgag gtggtgggcg
     6181 cgccaatgaa gtcgctccat cgcctgagga actgatcccg aatgtcgagc gattgctgcc
     6241 actgggcatc gtttggagtg ccccagagac taccgaggtc cgaaagcgct gcggcaatgc
     6301 gttcggcttt gccgggaaac atgccgatgg agtggtacag gaagtaccct gagccatcga
     6361 agtcgtgctc actcatttgc gaagcatggc atgtttcggc aggctgccgg catgagtaac
     6421 cactcggaag ccttcgacca ctggatccgc agctccttca tggagttgaa caccgaactg
     6481 gaaaacctct acttcgcgca ggacgatcgc tctgacgtca cccgcgccgg gcagaacctg
     6541 aagcaacagc tgcgtgagga aggccacgtg cacgtcgtcg cgctgctggc cgaaggcgac
     6601 accggcgacg gattcgccaa cgccttcggc gttctcggca acgttggcct gtacctggcc
     6661 gcgcttcgcc gccacgagtt gaccaatccc tcgcgcgaag agaaatcacc ttttccggag
     6721 gcgtcctcgc tcgcgatgca cgtcggtgcg gcgctcggca tggcgccccg attttccaca
     6781 gcacacatcg cgcacctgaa cttcgcgcat gcgggcgtgc gcaagagttt cacgtcgctt
     6841 ccggatgaaa tcctgttcat cgacgagaac acgcgcggca tcctgctgct gcagctcgca
     6901 gccgatgcgc tgacgaaggt cgtcccgctc ggcatatcga gcccgatcgt cgacgtgccg
     6961 ttcgcggccg cgaaacatgc gcttgctggc gtggtcgaat ccaacgaccg gctgtttgcc
     7021 agactcgacg tcgagcgctt tttcttttcc gtgcgcccgt attacaagcc atacagggtg
     7081 gggcggttcg agtatcgcgg tgcgaatgcc ggggattttt ccggcatcaa cgagctcgac
     7141 ctgttgttag gcctgtgtcg cgccaacgat ccgtattacg cacaactgct cgtcgacaag
     7201 atgcagttca tgccgcccgc agaccagaaa cggctgcgcg agtgcatgcg gcacacgagt
     7261 ttcctcgacg agctgctggc gctggcgggc aagcacgcgc gtgaggcctg gttccagcgc
     7321 aatgcacggg cgtacctgga actctgcggg ctgtacggcc gcatcgccgc gcagcaccac
     7381 gaccagctcg tgaagcgctt catcgagaat ccggcggcgc gtctcggcgc ggcgcatctc
     7441 gcggacatca cggcgagtgg cccgccgctg cctgtgctgg ttcgttctct cgaagtgctt
     7501 cgcgaccttc gactcgccgc gaatcggagc gacatcgcga cacggcacgc cgatttcgag
     7561 cagctgcggc gagtgctcga gccctgacgg ccgcgtcgcg ttgttggtcc gccctcagtt
     7621 cacgcgttgg aacgcgttcg cgaacacgta gtccgcaatg cggcgtccct gctggatgcc
     7681 cgccgtcttg tcgaacgccc agtgaatgcc gaggtagatg cggctctggc cgttctcctc
     7741 ttcggcttgc gacaaacgcg tgaagctgcg cggcaacaac ggccgcacct gcccgtcgtt
     7801 gcccacggtc tggccgttga actcatccga ggtgaacgtg aacgagaccc gatcggtgcc
     7861 gtaaacgttg cggagcattt cgaacagcgc ggcgccgaac gtagcgtggc cggagggata
     7921 cgcggggaaa ggcggagtga agttggggcc ggtcagattg ctcgccggtg cgccaagcgg
     7981 aaagaagccg gcatcaccga tcgtcgcggg attgctgtca ccggtgccgg aaggtccggt
     8041 gccggaatcc gattcacgaa tgcccgtgac gggacgccag aagtcgtaga agtatttcga
     8101 ctcccagccg gcgatgcccg cgtcggccat tgccacgttc aacaaggcat ataaccgggc
     8161 gagttgaatg acactcgtgt tgttcttgct gatctcggtc gcgatctggt tgtacagccg
     8221 cgggggagca cacaggctcg gcgttccgtc atatgcccag aagattccga tgtgggtctg
     8281 gtcagcgctg cgctccgtcg gcgtatttac gccatccccg ccgaggcgct tcacttcgtc
     8341 gtatgcggcg gtatatgcgg cgctcgtcat cgccggtggt ggcggcacgc ggaactgcga
     8401 tggcgagcgc atcacgaacg gtttcacttt gccccagtgt gccccgagcg cgatcggcgc
     8461 ctggctgatc gggtcctggc gccagtggcc ggccgcatcg ctggtgatcc attccgtatc
     8521 catgcgcgga tcggtccctg cccagccgtc gtcgcctcgc agtgccagaa tgcgcgcggc
     8581 agcgcgctgg cctaccgcca cgccaacgag cctctgacgg tcctgactcg ctggaacgcg
     8641 cgcgaggtcg cgcacgtaga gcgagttgaa attagcggtt tgcgaaggaa acattttgac
     8701 cagcgtgttg cgcgcggcgg tcgcgatggc cgcgtcgatc gaggcgggcc gggtctcgcg
     8761 cgccagcccc gtatagctgc gatacccgcc cgtgatggca ttgaccgcat cgaacacggc
     8821 gatgtgcacg atggctatcg cacgagccga ccggccgggg cctaactgct caccgaacac
     8881 ccggttctcg ccagcggcaa ccggcgtgtg gtcgacgccc gaagcgtcaa tggctatctg
     8941 attccaataa cgcagcgtgt cggctgtcga tgacgagcgt cgcgggggat tgatgaccgc
     9001 ggcggtcgtc gcggtagcag caatgcgcgg cccgtagacg tcactcgggc ccagcggctt
     9061 gtcggcggaa aaggattgcg tggccatcgc ggcgagaagc gacgcgccga cgctcgtggt
     9121 ccatcgtccg gaattggtgc tcatggtgac actcccttgt cagtccattg gtaatcgaac
     9181 gtaggcattt tttgactatt tcgaaacgaa ggaaacacgc gcgtgcgaaa agtgcgcgag
     9241 gcttgtactg tcgccggaaa cgaacatgtg tcctgcaatt ctttcaaagg aatacccatg
     9301 accaaccgtc gtcagttcct cggtgccgcg ctgtttgcat cggccgtgtt cacatcgctc
     9361 gcgcaaggcg cgtcgaaaac aggaatgctc cggcgacgca ttccgtcttc gggcgaactg
     9421 attcccgcca tcggcatggg tacctccgga agctttcaga tcccggcagg cagcccggaa
     9481 tacgacgccc ttaaggaagt cctcaagcga ttcttcgaag gcggggccac gctaatcgat
     9541 accgcgccga cctacggcaa cgccgaagac aacctcggac cgctgctcag cgagggcgga
     9601 tatcgcaaga aggccttcat cgcgacgaag ctctccggtg tcactggccg tgaagcaggg
     9661 ctcgagcaat tcaacaacac gctgcggcgg ctcaagaccg acaaggtgga gttgctccag
     9721 gtccacaacc tgcgcgattg gaaaacgcag ctcgaagtgg cgcgggagct caagaagcag
     9781 ggcaaggtga agtacgtcgg tgtgactcac tacaccgact ccgcccacga cgaaatcgcc
     9841 gacatcgtgc agcagagcaa gcccgacttc ctgcagatca accactcggt tacgaaccgc
     9901 ggcattgaaa agcgcgtgtt gccggtcgcc aaggagctgg gcgtcgcggt gctcacgaat
     9961 cgaaacttca acgacggcgc gttgtttggg caggtccagg gcaagccgct gcccggatgg
    10021 gctgccgatg cgggcgtcac ttcgtgggcg cagatgttcc tgaagtattc actgagccat
    10081 gaagccgtaa ccgtggtgat tcccgccacg ggcaagcccg agcgccagtc cgacaatctc
    10141 aaggcgggtg aaggaccact gctgacagct gcgcaacaaa aagagctgat cgcgctgttt
    10201 gcctgacggg tttcgtcatg caggtgtaat accgatctgg ctccatttgg cgcagcaata
    10261 acagaccggc ttcgtcgccg gcacaacgca cgcccggagg attccaagat ggtcgatttt
    10321 tccagccgct tggtcgcggc cgtcgtcgtt gcgacttgtg tcgtcgcacc cgcagccgca
    10381 gattcatcgc actggaagcg ccataaaaaa cgtgcgccgc tcgagctcag cgtgctgggc
    10441 acctattcga ccgggctcgg tgaagcaagc tcggagatcg cggcctacga cccgcaatcg
    10501 cgccgggcat tcgtcaccaa cgcggcagcc aggtccgtgg acatcctcga tgtccgcaac
    10561 tcgtcggctc ctacgcgcat cgaacgcatc gacgtcacgg cgctcggtac accgaacag
//
LOCUS       DASQJS010000468         6291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1565315, whole genome shotgun sequence.
ACCESSION   DASQJS010000468 DASQJS010000000
VERSION     DASQJS010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565315"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VEW08_18430"
     CDS             complement(<1..422)
                     /locus_tag="VEW08_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42773.1"
                     /translation="MIRRRLKIAALIFGLLLALVLGFLAWVLYTEAGLRFAVARLPER
                     MGKVTLRIERVYGTIAGGFGADLADIDSERTRVRVEKGTASVNLLPLLVGRISVRAAG
                     AELVEIEVKRRLNPPPKLPPRFLPRFLSISAETARSRML"
     gene            complement(419..2263)
                     /locus_tag="VEW08_18435"
     CDS             complement(419..2263)
                     /locus_tag="VEW08_18435"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HYJ42774.1"
                     /translation="MQIKLCPRLGRITLLALALAQLAVPANADVQIEVRGVGEDIRAN
                     VLAYLSFERYKNSDDLSPEFVERLQERSEREVRGALRPFGYYEPVVTSEVQREGGGGD
                     QNYRVIINIKPGSPVVVDKVDVKVTGPGAREKVFTDITGDLPIQTGDSLDHSNYEALK
                     GGLLRAAATNGYLDARMLRSEMRVDPQAHTAQIAIEFETGERYRFGPTTIRQDSIDDA
                     LIRRYVRYRENDDFDATELLRTQFALDDSQYFSTVEVLPEGRDRDSHIVPISIVAEPN
                     RRHRYSYGVGYGTDTQVRGTVAWEDRRVNKSGHRFRTEAKAAATAQSLDTRYVVPIGD
                     PATEKFQLQLTGEHEREGDIDDRTVNFIPSFTHTRGPWFGHDWQRVTYVEFLHAESEF
                     IASNRTDIQSLLIPGISVSLVPRKYLGEALFSRSLYAELRGSHNALGSDSDFLQVRMQ
                     AEHVFDLAPKWHVLMRGDIGATAVSRTSTLAPSQRFFAGGDRSVRGFGFNDLSPVEQA
                     TDANGDPLFDEDNNPVYEKVGGKHLFAGSVELIRDVGSRFAVAVFADAGNAFDSFDDP
                     LMYSAGIGVRFRLPVVSVGIDVAQALTTPAGSNEPPGPRLHLNFSPKL"
     gene            complement(2357..3148)
                     /locus_tag="VEW08_18440"
     CDS             complement(2357..3148)
                     /locus_tag="VEW08_18440"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HYJ42775.1"
                     /translation="MCLLVLAWRSHPQHRLVVAANRDEFHARPAAPLAPWPDIAGVVG
                     GRDLQAGGAWFAIGDAQRLGIVTNFREFGRQRRNAPSRGGLIPAYLSGALQPGDFLQG
                     LETDAQGYSGFNLLLADRDTLWYASNRADQFARELPPGIYGLSNEFLDTPWPKLVRVR
                     ARFEALLQSGESADPDELATRLLVMLADRETAPPDTLPPGDLTPEWARKLSAPFVLDP
                     SYGTRCSTALTISAQGTLRITERRFDAEGARTGQSEHLLNGTGES"
     gene            complement(3155..4069)
                     /gene="nudC"
                     /locus_tag="VEW08_18445"
     CDS             complement(3155..4069)
                     /gene="nudC"
                     /locus_tag="VEW08_18445"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HYJ42776.1"
                     /translation="MTIRPTLFAGPFLERRAELRDDPAWVAAARGDSTTRYLLTAGAT
                     QLVTGGAIAEVAFLEGGHPLVAAARDEELTLLGWFRGARCVLVELQDPGAALPADTEL
                     RELRPLAPLLPPDSASLLAYARALALWKARHRHCGVCGTPNYPARAGHVMRCSRAGCG
                     TEAFPRLDPAIIVLVTDSTGERALLGRQATWPEGRYSTIAGFVEPGESLEDAVIREVE
                     EETGVVAGDVEYVASQPWPFPSSLMLGFRAVARTHDITLRDGELEDARWFSRADLAAG
                     HPKLPPAGAISARLIDAWYGAPEPRSRG"
     gene            complement(4131..4721)
                     /locus_tag="VEW08_18450"
     CDS             complement(4131..4721)
                     /locus_tag="VEW08_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42777.1"
                     /translation="MRISDDRYSRDRLRLDLALRFIHHEARTHTIRAWTGLTDDRIRK
                     LYRTYLYEAGGATVARHRGKSPRQAAFFTRSLRMRREAAVFASVSSLFGLITPQTITV
                     SPSAVAREAASAPTVARGALLCEAFEAYTALMGESQISFEHAVFLLGALLAGDELRVA
                     HCRCCSGVLVTDRLALRVPVCNECAASDPHCEVQAD"
     gene            4843..5499
                     /locus_tag="VEW08_18455"
     CDS             4843..5499
                     /locus_tag="VEW08_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42778.1"
                     /translation="MTSRGGQRTRDDVRHWLPSQGVDPEPLAALNSECLGQLVLRAAE
                     SDNIASAPLLLSSLRVQWASLPAQAVRRLSAAPFSLFDAGFASAPRWLEMRTRGVNEL
                     PQRVEPAYFPADMARAITRRMLVYGWHLARSRPRAACLVFGATMAGVESLAACSLVSL
                     EAAADRNAGALRPRWANQTTFWRELLLAASAGADRRLHELLLGGVQRLAAETLAAHPL
                     "
     gene            5602..>6291
                     /locus_tag="VEW08_18460"
     CDS             5602..>6291
                     /locus_tag="VEW08_18460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42779.1"
                     /translation="MTITRSAIAAVAAMAVFISFGAHGATRAEALAAMKAAKSGDGAR
                     LGASLAVLQRAAARRLPQSDVTHRVTRKLPALRASDGYVSISAYGDDLQGLRTQLTAK
                     GLIDGVVHSTAVSGKAPVAALGDMAATTGLKFLRPTLAMARAGATLSQGDRSLRADLA
                     RRESGVSGRGIRIGVLSDSYDCAPGAFEPGAPFTRAAQDMANDDLPRDVLVLKDLADA
                     PSDDCSDEGRAM"
BASE COUNT         1052 a         2031 c         2163 g         1045 t
ORIGIN      
        1 agcatgcgcg agcgagccgt ctcggcgctg atgctcagga accgtggcag aaacctgggt
       61 ggcaatttcg gtggcgggtt cagccggcgc ttgacctcga tctccacgag ttcggcgccc
      121 gcggcgcgca ccgagatgcg ccccaccagc agcggcagga gattgacgct cgccgtgcct
      181 ttctcgacgc gcacgcgagt gcgttcgctg tcgatgtcgg caaggtcggc accgaaaccg
      241 ccggcgatgg ttccgtagac gcgttcgatg cggagggtga ccttgcccat gcgttcgggc
      301 aggcgcgcga cggcgaaacg caatccagcc tcggtgtaga gcacccaggc gaggaatccc
      361 agcaccagag ccaacagcaa cccgaagatg agggccgcaa tcttgagtcg ccgcctgatc
      421 acaatttggg cgagaaattg aggtggaggc gcggaccggg tggctcattg ctgcccgcgg
      481 gcgtggtcag cgcctgcgcg acatcgatac ccacggaaac caccggaagg cgaaatcgca
      541 cgccgatacc tgcggaatac atcaatggat cgtcgaaact gtcgaacgcg ttgccggcgt
      601 cggcaaacac cgccacggcg aaccgcgagc cgacgtcgcg tatgagttcc accgagcccg
      661 cgaacaggtg tttgccgccg accttttcgt agaccgggtt gttgtcttcg tcgaagagcg
      721 gatcaccgtt ggcgtcggtg gcctgctcaa cgggcgaaag atcattgaag ccgaagccgc
      781 gcacgctgcg atcgccgccc gcaaagaacc gttgtgaggg tgcgagagtg ctggtgcgcg
      841 agaccgcggt ggcgccgatg tcgccgcgca tcagcacatg ccacttcggc gccaggtcga
      901 aaacgtgctc cgcctgcatg cgtacctgca ggaagtcgga atccgaaccg agcgcgttgt
      961 gcgacccgcg aagctccgcg tagagcgagc ggctgaacag cgcctcgccg agatacttgc
     1021 gcggcaccag cgagacactg atgcccggga tcagcagcga ctggatgtcg gtgcggttcg
     1081 acgcgatgaa ttccgattcc gcgtgcagga attctacgta cgtcacgcgc tgccagtcgt
     1141 ggccgaacca cggcccgcgc gtgtgcgtga agctgggaat gaagttgacg gtgcgatcgt
     1201 cgatgtcgcc ttcccgctcg tgttcgccgg tgagctgcag ctggaatttt tcggtggccg
     1261 gatcgccgat gggcacgacg tagcgcgtgt cgagtgactg cgcggtcgcg gcggccttcg
     1321 cttccgtacg gaagcgatga ccgctcttgt tcacgcgccg gtcttcccat gcgaccgtgc
     1381 cgcgcacctg ggtgtcggtg ccgtagccca cgccatagga gtaccgatgg cggcggttcg
     1441 gttcggcgac gatgctgatg ggcacgatat ggctgtcgcg atcgcgccct tcgggaagca
     1501 cttcgacggt ggagaaatac tggctgtcgt cgagcgcgaa ctgtgtgcgc agcagttcgg
     1561 tggcgtcgaa atcgtcgttc tcgcggtaac gtacgtagcg gcggatcagc gcgtcgtcga
     1621 tgctgtcctg cctgatggtg gtggggccga agcgataacg ctcgccggtt tcgaattcga
     1681 tggcgatctg cgccgtgtgg gcctgcggat ccacgcgcat ttcgctgcgc agcatgcgtg
     1741 cgtcgaggta gccgttggtt gccgcggcgc gcagcagccc gcccttgagc gcctcgtagt
     1801 tagagtggtc gaggctgtcg ccggtctgga tgggcaggtc gccggtgatg tcggtgaaca
     1861 ccttctcgcg cgcacccggg cccgtcacct tgacgtccac cttgtcgacg accactggac
     1921 tgccgggctt gatgttgatg atgacgcggt agttctggtc accgccgccg ccctcgcgct
     1981 gcacctccga tgtgaccacg ggttcgtagt agccgaaggg acgcaaggct ccgcgcacct
     2041 cgcgctcgct gcgctcctgc aggcgctcga cgaactcggg cgaaaggtcg tcggaattct
     2101 tgtagcgctc gaacgataga taggcgagca cgttggcgcg gatgtcctcg ccgacaccgc
     2161 ggacctcgat ctgcacatcg gcgttcgccg gaaccgcgag ttgcgcgagg gcgagcgcga
     2221 gcagcgtgat acggccaagg cgcgggcaaa gtttgatctg caaggtcggc atgacaccgc
     2281 tttggggcgg gaaggctgaa gattctaatg agagtcccgc tggcgcgcgc gggttccgcg
     2341 cgcattcgcg acgtggtcaa ctctcgccgg ttccgttcag caggtgttca gactgtcccg
     2401 ttcgtgcgcc ttcggcatcg aagcggcgct cggtgatccg cagcgtgccc tgggccgaaa
     2461 tcgtgagtgc cgtcgagcag cgggtgccat aactcggatc cagcacgaac ggcgcggaca
     2521 gcttgcgtgc ccattcgggt gtgaggtcgc cgggcggcag ggtgtcgggc ggtgccgttt
     2581 cgcgatccgc cagcatcacc aacagcctgg tggccagttc atcgggatct gcggactcgc
     2641 ccgattgcag gagggcttcg aaacgtgccc gaacgcgcac gagttttggc catggcgtgt
     2701 cgaggaattc gttggagagg ccatagatcc caggcggcaa ttcgcgcgca aactggtccg
     2761 cgcgattcga tgcgtaccag agcgtgtcgc ggtcggccag cagcagattg aagcccgagt
     2821 atccctgcgc atccgtctcc agcccctgca gaaaatctcc gggctgaagc gcgccggaga
     2881 ggtatgcggg gatcagcccg cctcgcgacg gagcgttgcg ccgctggcgc ccgaactcgc
     2941 ggaagttggt gacgataccg aggcgctgcg catccccgat cgcgaaccat gcgccgcccg
     3001 cctgcagatc gcggccaccg acgacgccgg cgatgtccgg ccagggcgcg agcggggcgg
     3061 cgggccgcgc atggaattca tctcggttgg cggcgaccac caggcggtgc tgcggatggc
     3121 tccgccaggc gaggactaac aaacacatgg gcggctatcc gcggctgcgt ggctcggggg
     3181 cgccgtacca ggcatcgatg agccgcgcgg agatcgcgcc cgccggcggc agcttggggt
     3241 gccccgcggc gaggtcggca cggctgaacc agcgtgcatc ttcgagctcg ccgtcgcgca
     3301 gcgtgatgtc atgggtgcgc gccaccgccc ggaaccccag catcagcgac gacgggaacg
     3361 gccagggttg agaggcgaca tactcgacgt cgccggccac cacgccggtc tcttcctcga
     3421 cctcacggat cactgcgtct tcgaggcttt cgccgggttc gacgaaaccg gcgatagtcg
     3481 aatagcggcc ctccggccac gttgcctgcc ggcctaacaa tgcgcgttcg ccggtggagt
     3541 cggtgaccag cacgatgatc gccgggtcga gccgtggaaa cgcctcggtg ccgcaaccgg
     3601 cccggctgca gcgcatgaca tggccggctc gtgccgggta atttggggta ccgcataccc
     3661 cgcaatgccg gtgccgggcc ttccacagcg ccagcgcacg tgcatacgcg agcagagatg
     3721 cggagtccgg cggcagcaag ggcgccagcg gtcgcaactc ccgcaattcg gtgtccgcgg
     3781 gcagcgctgc accgggatcc tgcagttcga cgagcacgca ccgcgcgccg cgaaaccagc
     3841 ctagcaatgt cagctcttcg tcgcgggcag cggccaccag cgggtgaccg ccttcgagga
     3901 aggcgacttc cgcgatcgcg ccgccggtca cgagctgggt cgcaccggcg gtcagcagat
     3961 aacgcgtggt ggagtcgccg cgcgcggcgg cgacccacgc cgggtcgtcg cgcagctcgg
     4021 cacggcgctc gagaaacggg ccggcaaaca gcgttgggcg aatggtcacg cggacaaatc
     4081 taccgcaccc gtcgcccgcg cgccccctga cccggcgccg ctgtacggcc tcagtccgcc
     4141 tggacttcgc aatgcggatc gctcgccgcg cattcgttgc aaaccggaac gcgcagggcg
     4201 aggcggtcgg tgaccaggac gccgctgcag caacggcagt gggcgacccg caattcgtcg
     4261 ccggcgagca gcgccccgag caggaatacg gcgtgctcga aggagatctg cgactcgccc
     4321 atcagtgccg tatatgcctc gaaggcctcg cacaacagcg caccgcgcgc gaccgtgggc
     4381 gcggacgccg cctcccgcgc caccgccgaa ggtgaaacag taatggtctg cggagtgatc
     4441 aggccgaaca ggctgctcac gctggcgaag accgccgctt cgcggcgcat tcgcaacgaa
     4501 cgcgtgaaga aagctgcctg gcgcggcgac ttgccgcggt gccgcgcgac cgtggcgccg
     4561 cccgcctcgt agaggtaggt gcgatagagc ttgcggatgc gatcgtcggt gaggccggtc
     4621 cacgcacgga ttgtgtgcgt ccgggcttcg tggtggatga agcgcagcgc gagatcgagg
     4681 cgcaggcggt cgcggctgta gcggtcgtcg gagatgcgca tgtttttccc tggtgccatg
     4741 tggattgtga ttgtcgacaa tttgggaaaa atttcccaaa tttgcaaatg actttcaagg
     4801 ggtggcggcg caagatatct cgcgcgttta gggaggaatg ccatgacaag tcggggaggt
     4861 cagcgcacca gggatgacgt gcgccactgg ttaccgtcgc agggggtcga tccggagccg
     4921 ctggcggcgc tcaactcgga atgcctcggg cagttagtcc tgcgcgcggc tgagagcgat
     4981 aacatcgcgt ccgcaccgct gcttttgtcg tcgttgcgcg tgcagtgggc atcgctgccg
     5041 gctcaggccg tgcgccggtt gtccgcagcg ccattcagcc tgtttgacgc cggctttgct
     5101 tcggcgccgc gctggctcga gatgcgaacg cggggcgtca acgaattgcc gcaacgcgtc
     5161 gagcccgcgt atttcccggc cgacatggcg cgggccatca cgcgtcgcat gctggtctac
     5221 ggctggcacc tcgcgcgcag ccggccgcgc gcggcgtgcc tggtcttcgg tgcgaccatg
     5281 gccggcgtgg aatcactcgc ggcctgctcg ctggtttctc tcgaggccgc tgccgatcgc
     5341 aacgccggag cgttgcgacc gcgctgggca aaccagacca ccttctggcg agagctgctg
     5401 ctcgccgcgt cagcgggcgc cgaccggcgt ctgcacgaac tactgctcgg cggcgttcag
     5461 cgccttgccg ctgaaacact cgcggcgcat cctctttgac acaacctgcg cgcgctgctc
     5521 gatgaagaaa cgcgcgtgca aggagaagcc accgaactct gcggacggag cccgcgcatt
     5581 caacttaact cggggagcaa catgaccatc acgagatcag ccatcgcggc cgtggccgcc
     5641 atggcggtgt tcatttcctt tggtgcacac ggcgcaaccc gcgccgaagc actggccgcg
     5701 atgaaagccg cgaagtccgg cgatggcgcc cggcttggcg ccagtctcgc ggtactgcaa
     5761 cgggcggcgg cgcgtcgcct gccgcagtcc gatgtcaccc accgcgtaac gcgcaaattg
     5821 cccgcgcttc gcgcaagcga tggatacgtc tccatcagcg catacggaga tgatctgcag
     5881 ggcctgcgca cacaactcac cgccaagggc ctcatcgacg gcgttgtgca ttccacggcc
     5941 gtgtcgggca aggcgccggt cgcggcactg ggtgacatgg ccgccaccac cggtctcaaa
     6001 tttctgcggc ccacactggc catggcgcgt gccggcgcaa ccttgtccca gggcgatcgg
     6061 tcgttgcgtg ccgatctcgc ccgccgtgaa agcggagttt cgggacgcgg gattcgcatt
     6121 ggcgtgttgt ccgacagcta cgactgcgca cctggcgcgt tcgagcccgg cgcgccattc
     6181 acgcgcgcgg cgcaggacat ggccaacgac gatctgccgc gcgacgtgct cgtcctcaag
     6241 gacctcgccg atgcgccgag cgacgactgc tccgacgaag ggcgcgccat g
//
LOCUS       DASQJS010000469        10816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2825970, whole genome shotgun sequence.
ACCESSION   DASQJS010000469 DASQJS010000000
VERSION     DASQJS010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10816
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2825970"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VEW08_18465"
     CDS             <1..318
                     /locus_tag="VEW08_18465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42780.1"
                     /translation="ANKPERFLQSATALFGTNAPFMPALIPMSATLLPYLQKSDPAMP
                     PPAALSLLAAVLGVWLLVVQVRILRTAFEWHWVAALIFFFALNFASALVYGMLFGFPS
                     THV"
     gene            335..1705
                     /gene="mpl"
                     /locus_tag="VEW08_18470"
     CDS             335..1705
                     /gene="mpl"
                     /locus_tag="VEW08_18470"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HYJ42781.1"
                     /translation="MHVHILGICGTFMGGLAAIAKAAGHRVTGSDKNVYPPMSTQLEA
                     LGIELISGFEASQLSPKPDIVVVGNVMTRGAPVIEALLESRIPYTSGPEWLAREVLRD
                     VHVLGVSGTHGKTTTSSMLAWILEHAGLNPGFLIGGVPGNFSVSARLGGRKYFVIEAD
                     EYDTAFFDKRAKFVHYRPRTLVLNNLEFDHADIYENLGAIRKQFHHLVRTIPGSGRIL
                     WNSGDDNLKATLAMGAWTPLEGFARTPGAEAAWSLDVEEGGDYSKFVVLEGGKPAGTV
                     KWSLLGAHNAENALAAIGAARHAGVSVAQSIAALADFKGVKRRMELRGEVRGIAVYDD
                     FAHHPTAIAFTIDGLRRKVGGKRIVAVLEPRSATMRMGVHRDTLAPSLAGADAVWMFA
                     PPDLGWDADAVVATLGAKGRLARSVDELVSGLAGSTASGDQVLIMSNGGFGGLHGKLL
                     EALARM"
     gene            1722..2309
                     /locus_tag="VEW08_18475"
     CDS             1722..2309
                     /locus_tag="VEW08_18475"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HYJ42782.1"
                     /translation="MNEPSVIALFPLHTVLFPGGPLPLRIFETRYTDMVRRCMREQQP
                     FGVVLIQEGDEAGDVATTATVGCTARIADFHTLHDGFLGISCVGMHKFRVLRVWRASD
                     GLNMGEISWLPAEPELALPERYAHLVDTVRRALEDLSEHYEHVERKFDDAAWIGARLT
                     ELLPIELADKQALLELDDPIERLDTLLTVVPAPNS"
     gene            complement(2306..3166)
                     /locus_tag="VEW08_18480"
     CDS             complement(2306..3166)
                     /locus_tag="VEW08_18480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HYJ42783.1"
                     /translation="MIALGLAGIAFLGYPAWLVMAPWLDNPKFHRIASRVGMLLLAIG
                     FVIVVRRLKMADRQSLGFGLPAEKFVAGVFKALVLGIVLMMPALMTMLLLDMREPAGE
                     PMNALQWVQLALGAIITGLTVALIEETFLRGAMQFAITRESGARVAVVLTSLVYAATH
                     FIGRYRVRAADVHAGSGIDMLAFALADFAQPLHFIDAFLCLVAVGLLLGMARVYTGNI
                     AACIGLHAGWVAVIYVVNETSKREVGSPLSWMLSDVNGFLGWMVLAWTMAIGLAMSFW
                     YRQRPQRTIP"
     gene            complement(3202..4119)
                     /locus_tag="VEW08_18485"
     CDS             complement(3202..4119)
                     /locus_tag="VEW08_18485"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HYJ42784.1"
                     /translation="MIFPEAMSRGLAHGLAGVLLLAFGAQVAHARGETSVWSMKGTRN
                     TVYLAGSVHALPKGHSDFPEQLERAYMASDVIVLEVDLDDMNPLDAVKFITTNGTLPA
                     DKSLKDVVGAEPYQRIAALAASLEVPEMVIAKLEPWAAALVLTQFALNKTGFDANLGI
                     DMQITERARTDGKPVEGLETVIDQLSVFDDRSFEEQTRFLLDSADDAPQLNDDLQKLI
                     AAWRAGDLRALEKEFLKEREKSPELYDALLGVRNRLWLPKIKALLEEDRDYLVVVGAL
                     HFVGREGLLALLRSDGHKAVAVPAAKTPR"
     gene            complement(4116..4883)
                     /gene="cpdA"
                     /locus_tag="VEW08_18490"
     CDS             complement(4116..4883)
                     /gene="cpdA"
                     /locus_tag="VEW08_18490"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: protein motif:HMM:NF008359.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HYJ42785.1"
                     /translation="MSAIRVIQFTDPHLFGDIAGTLRGVNTYETLERTLADASGHIAM
                     SDAILVTGDLVQDDAGGYEHFRRLFARFGKPVLCIPGNHDFVPQMREALAGRPFELDG
                     PADIGAWRIVLLDSVVPEQAGGRISVEMLTKLDEELGRARQRHAMVCLHHHPIAMHSR
                     WLDSVGLSNADEFWSVIDSHPQVRAVVWGHVHQDFEGARRGVRLIATPSTCAQFQPRS
                     DQFVIDTQPPAYRLLTLHSNGAIDTGVHSLTAAESAA"
     gene            5140..5850
                     /locus_tag="VEW08_18495"
     CDS             5140..5850
                     /locus_tag="VEW08_18495"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ42786.1"
                     /translation="MKRPGKNDPSRPREGDYDYKREFYRSSSVAEDYDFHRFSSPERQ
                     KRNRRKWAAISRALREATGVRTILDLPCGTGRFTGALAREGFEIVGSDISMEMLHKAA
                     SLGNENKADGQHPAIRGYVQANAEHLPLRNDSLDCVVSIRFMMHVDPVTRVRMLREFH
                     RVSRRWVVIDYRHCYSFRYVLTHTLGRLGIGRPPLSRVSRKELEKEFADAGFAIRNVI
                     RVSAPLLSDKWIVLAERA"
     gene            complement(5857..6327)
                     /locus_tag="VEW08_18500"
     CDS             complement(5857..6327)
                     /locus_tag="VEW08_18500"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYJ42787.1"
                     /translation="MKLESNVFDVWVFRRTPAQVEYLLLHTSQLKADRYFNGGRFWQI
                     PSGFMQDSERVVPAIVRLLADFGIAATGIWAAEHVYNIYNRRFGTMQMIAVYAAEAGK
                     TELRLDPQEHAESRWCPYEEALELVNYRGLKDGLRSTREYVTGLEAAAAELRLM"
     gene            6471..8471
                     /gene="tkt"
                     /locus_tag="VEW08_18505"
     CDS             6471..8471
                     /gene="tkt"
                     /locus_tag="VEW08_18505"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HYJ42788.1"
                     /translation="MTPTRRALANAIRALSMDAVQAANSGHPGAPMGMADIAEVLWRD
                     VLKHNPGNPKWWNRDRFVLSNGHGSMLLYSLLHLTGYPLAIDELKKFRQLGARTAGHP
                     ERDLEIGIETTTGPLGQGFANAVGMALAEKLLAAQYNKDGAAIVDHHTYCFVGDGCLM
                     EGISHEAASFAGHLKLGKLIVFYDDNGISIDGKVAGWFTEDTPKRFEAYGWHVVKNVD
                     GHDADAVAAAIKKAKNRKDQPSIICCKTTIGWGAPNKQGTKSAHGEALGPDEVANARK
                     TLGWESPPFVIPEDIRAAWDHREAGAAAEKKWLRVWRRYKREFPKEGAEFERRMKGDL
                     PADWAEIVQAAIAAGAAVTGAQATRASSQAVLNVLGPKMPEMLGGSADLTGSVNTKRK
                     DSVDLTTENFHGNYVYYGVREFGMTAIMNGITVHGGYRPYGGTFLVFSDYARNAVRVA
                     AIMHCPTTLVYTHDSIGLGEDGPTHQPIEHLASLRAMPNLHTWRPCDAVESAVAWTSA
                     LERKNGPTALIFTRQGLPQQARTPEQLVALRKGGYVLIDSNGPPEAIVIATGSEVGFA
                     AEAVKELQAAGKRVRLVSMPCCEAFDAQPAPYRESVLPASCMRRLAVEAGATQSWWRY
                     VGSQGRVMGIESFGASGKGPDLFKHFGLTAADLKAQVEQMLA"
     gene            8530..9543
                     /gene="gap"
                     /locus_tag="VEW08_18510"
     CDS             8530..9543
                     /gene="gap"
                     /locus_tag="VEW08_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HYJ42789.1"
                     /translation="MAIKVGINGYGRIGRNILRALYEAKRNNEIQIVAVNDLGDAQTN
                     AHLTQYDTAHGKFPGTVSVEGDSIVVNGDKIKVFAQRDPSKIDWASVGAEYVFESTGF
                     FTTKADAGKHLAGGAKKVVISAPGGKDVDATVVYGVNHNVLKASHTVISNASCTTNCL
                     APLAKVLNDKIGIVSGLMTTIHAYTNDQRLTDVYHEDIRRARSATMSMIPAKTGAAAA
                     VGLVLPELKGKLDGFAIRIPTINVSLVDLTFVAKRATTVEEINKVVKEAADGAMKGVL
                     AYTDKQLVSIDYNHDPASSTYDSTLTRVTEGTLVKVCSWYDNEWGFSNRMLDTTIAWS
                     KAS"
     gene            9555..10733
                     /locus_tag="VEW08_18515"
     CDS             9555..10733
                     /locus_tag="VEW08_18515"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005896277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HYJ42790.1"
                     /translation="MNVLRMADQDLAGKRVLIREDLNVPVEGGKVTSDARIRASLPTI
                     KLALEKGAKVFISSHLGRPEEGVYAEEFSLAPVAARLSDLLGKPVLLKKDWLLGVDTP
                     AGSAVLLENVRFNKGEKKDKEELARQMASLCDIYVMDAFGTSHRAEASTHGVGKYAKV
                     ACAGPLLVGELEALERALAKPARPLVAIVAGSKVSTKLTVLESLLAKVDKLIVGGGIA
                     NTFLAATGINVGKSLHEADMLDMARKLLDAARARGAEIPLPTDVVVAKEFAATAHADI
                     RSIHEVRPDEMILDIGPDSAERFGKMLQTAGTIVWNGPVGVFEWDQFGEGTKAIAQAI
                     ARSPAFSLAGGGDTLAAIEKYGVEEDISYISTGGGAFLEFVEGKTLPAVAMLESRAKQ
                     "
BASE COUNT         2031 a         3448 c         3445 g         1892 t
ORIGIN      
        1 gcgaacaagc ccgaacgctt cctgcagtcc gccacggcgc tctttggcac caacgcgccc
       61 ttcatgcccg cgctcatccc gatgagcgcc acgctgctgc cgtatctgca gaaatccgat
      121 cccgcgatgc caccgccggc cgccctgagc ctgctcgcgg ccgttctggg cgtctggctg
      181 ctggtcgtgc aggtccgtat cctgcgcacg gcgttcgagt ggcattgggt cgccgcgctc
      241 atcttcttct tcgcgctgaa cttcgcttcg gcgctggtgt atggaatgct gtttggattt
      301 ccgtccactc acgtctagag gctagagtcc ggtcatgcac gttcacattc tcggcatctg
      361 cggcacgttc atgggtggcc tcgccgccat cgccaaggcc gcgggccatc gtgtcacggg
      421 ttcggacaag aacgtctatc cacccatgag cacacagctc gaagcgctcg gcatcgagct
      481 catctcgggc tttgaagcca gccagctctc gccgaagccg gacatcgtgg tggtgggcaa
      541 tgtcatgaca cgcggggcgc cggtcatcga ggcgctgctc gaaagccgga ttccgtacac
      601 ctcggggccc gaatggctgg cgcgcgaagt gttgcgcgac gttcacgtgc tcggtgtttc
      661 cggaacgcac ggcaagacca ccacctcgag catgctggcc tggatactgg aacacgccgg
      721 cctcaatccc ggcttcctca tcggtggcgt gcccggtaat ttttcggtga gcgcgcgcct
      781 cggcgggcga aaatatttcg tcatcgaagc cgatgaatac gacaccgcgt tcttcgacaa
      841 gcgcgcgaag ttcgtccact accgtccgcg cacgctggtt ctgaacaacc tggagttcga
      901 ccacgccgac atctacgaaa accttggcgc catccggaag cagttccatc acctggttcg
      961 caccattccg ggctcgggcc gcattctctg gaattccggc gacgataacc tgaaggccac
     1021 gctcgcgatg ggcgcgtgga cgccgctcga aggattcgcg cgcacgcccg gcgccgaggc
     1081 ggcgtggtcg ctcgacgtgg aagagggcgg cgactactcc aagttcgtgg tgctcgaagg
     1141 cggcaagcct gccggcacgg tcaagtggtc attgttaggt gcgcacaacg ccgagaacgc
     1201 gctggccgcc atcggcgccg cgcggcacgc cggggtttcc gtggcgcagt ccattgcggc
     1261 gctcgcggat ttcaagggcg tcaaacgtcg catggagctg cgcggcgaag tccgcggcat
     1321 cgcggtgtac gacgatttcg cgcatcaccc cacggcaatt gccttcacca tcgatggctt
     1381 gcggcgcaag gtcggcggca agcgcatcgt cgccgtgctc gagccgcgct cggccaccat
     1441 gcgcatggga gtgcatcgcg acacgctggc gccttcgctc gcgggtgcgg atgcggtgtg
     1501 gatgttcgcg ccgccggatc tcggctggga cgccgatgcc gtggtggcca cgctcggtgc
     1561 caaggggcgg ctggcgcgat cggtcgatga gctggtgagc ggcctcgcgg gcagcaccgc
     1621 gagcggcgac caggtactca tcatgagcaa cggcggcttc ggcgggctgc acggcaaact
     1681 actcgaggcc ctggccagga tgtgaccgag catgtgatcc agtgaacgag cccagcgtca
     1741 tcgcgttgtt tccgctgcac accgtgctct ttcccggcgg cccgctgccg ctgcgcatct
     1801 tcgaaacccg ctacaccgac atggtccggc gctgcatgcg cgagcaacag ccattcggcg
     1861 tggtgctcat ccaggagggt gatgaggctg gcgacgtcgc caccaccgcc acggttggtt
     1921 gcacggcgcg catcgccgat ttccacacgc tgcacgacgg atttctgggc atcagttgcg
     1981 tgggcatgca caagttccgc gtgttgcgcg tgtggcgcgc cagtgacggg ctcaacatgg
     2041 gcgagatctc atggctgccg gccgaaccag agctggcgct tccggagcgg tacgcgcatc
     2101 tggtggacac ggtgcggcgg gcactcgaag acctctccga acactacgaa cacgtcgaga
     2161 ggaaattcga cgacgccgcc tggattggtg cgcggctcac cgaactactg cccatcgagc
     2221 ttgccgacaa gcaggcactg ctcgagctcg atgacccgat cgagcgactc gataccctgc
     2281 tgaccgtcgt acccgcgccg aatagctagg gaatggtccg ctgcggccgc tgccggtacc
     2341 agaaggacat cgccaggccg atggccatcg tccaggccag caccatccag ccgaggaagc
     2401 cgttgacgtc gctgagcatc cagctcaatg ggctgccgac ctcgcgcttc gatgtttcgt
     2461 tcaccacgta gatcaccgcg acccaacctg cgtgcagccc gatgcacgcg gcgatgttgc
     2521 cggtatagac gcgcgccata ccgagcagga ggccaactgc caccaggcaa aggaaggcgt
     2581 cgatgaagtg caatggctgg gcgaaatcgg ccagcgcgaa cgccagcatg tcgatgccgc
     2641 tgccggcatg cacatcggcg gcacgaaccc ggtatcggcc gatgaagtga gtagccgcgt
     2701 acaccagcga agtgagcacg acggccaccc gcgcgcccga ctcgcgcgtg atggcgaact
     2761 gcatggcgcc gcgcagaaag gtttcttcga tgagcgcgac agtgaggccg gtaatgatgg
     2821 cgcccaacgc gagttgcacc cactgcagcg cattcatggg ctcgccggcc ggttcgcgca
     2881 tgtcgagcag cagcatcgtc atgagcgcgg gcatcatcag cacgatgccg agcaccagtg
     2941 ccttgaacac cccggcgaca aacttttctg cgggcaggcc gaaccccagg ctttgccggt
     3001 cggccatctt gagccggcgc accacgatga cgaagccgat cgccagcagc agcatgccga
     3061 cgcggctcgc gatgcgatga aacttggggt tatcgagcca tggcgccatc accagccagg
     3121 ccgggtagcc aaggaaggcg atgccggcga ggcccagcgc aatcaatcct gtgaagatcg
     3181 cgaaggctcg caatctgggg gtcagcgagg tgtcttggcg gcaggtaccg caacggcctt
     3241 gtggccatca ctcctgagca atgccagcag gccttcgcgg cccacgaagt gcagagcgcc
     3301 aaccaccacg aggtagtcgc gatcttcttc gagcagcgct ttgattttcg gcagccacag
     3361 gcgattgcgc acgccgagca gagcgtcgta gagctccggt gacttctcgc gctccttcag
     3421 gaattccttc tcgagggcgc gcaggtctcc ggcgcgccag gcggcgatga gcttctgcag
     3481 gtcatcgttg agttgcggtg cgtcgtcggc ggaatccagc aggaagcgcg tttgttcctc
     3541 gaagctgcga tcgtcgaaga cgctcagctg atcgatgacg gtttccagcc cctcgacggg
     3601 cttgccatcg gtgcgcgctc gctcggtgat ctgcatgtcg atgccgagat tcgcgtcgaa
     3661 cccggtcttg ttgagcgcga actgcgtgag taccagcgcc gccgcccagg gctcgagctt
     3721 tgcgatgacc atttcgggca cttcgagcga ggcagccagc gcggcgatgc gttgataagg
     3781 ctccgccccc accacgtcct tgagggactt gtcggcaggc aacgtgccgt tcgtggtgat
     3841 gaacttcacc gcgtcgagcg ggttcatgtc atcgagatcg acttccagca cgatgacatc
     3901 ggacgccatg tatgcgcgtt cgagctgctc gggaaaatcg gaatggcctt tcggcagtgc
     3961 gtgcaccgaa ccggcgagat agaccgtgtt gcgcgtgcct ttcatcgacc agactgacgt
     4021 ttcgccgcgc gcgtgtgcaa cctgggcgcc aaacgcgagc aacagcacac ccgcgaggcc
     4081 gtgggcgagg cctctggaca tcgcttcagg aaaaatcatg ccgcggattc tgcggcagtc
     4141 agcgagtgca cgccagtgtc gatggcgccg ttggagtgga gggtgagcag ccgataggcc
     4201 ggcggctgag tgtcgatgac gaactgatcg gagcgcggct ggaattgcgc gcaggtggaa
     4261 ggtgtcgcaa tcagtcgcac gccacgacgc gcgccttcga agtcctggtg cacatggccc
     4321 caaaccaccg cgcgcacctg gggatgactg tcgatgaccg accagaactc gtccgcattg
     4381 gacaagccga cgctgtcgag ccaacggctg tgcatcgcga tcggatggtg atgcaggcag
     4441 accatggcgt ggcgctggcg cgcgcggccc agctcttcat cgagtttggt cagcatttcg
     4501 acggagatgc gaccgccggc ctgttcgggc accacgctgt cgagcagcac gatgcgccag
     4561 gcgccgatgt cggcggggcc gtccagctcg aaggggcggc ccgcaagcgc ttcgcgcatc
     4621 tgcggaacga aatcgtgatt gccgggaatg cacagcaccg gcttgccgaa acgggcgaac
     4681 aggcgtcgga aatgctcgta gccgccagcg tcgtcctgta ccaggtcgcc ggtgaccagg
     4741 atggcatcgc tcatggcgat atggcccgac gcatcagcga gcgtgcgctc cagcgtctcg
     4801 taggtattca cgccacgcag cgtgcccgcg atatcgccaa acaaatgggg gtccgtgaac
     4861 tggatgactc tgattgcact cattcattcg aactctacaa acatgccggg gaggggccgg
     4921 tgatgcacgt cacgacatgg ttcatggcgc tgtaacgtgg tgaaccaggt cgcaaaccac
     4981 ccccggaata atgtgaaaca gagtcgtcgt ctagcgactt tccgaagatg taaccgctat
     5041 caaaacaatg gtaaatcgct gcggtagagt ccatagggaa ctgaccctat tgctcttcca
     5101 agattcccga cccgctagcg cgctaaagtg tcagtactta tgaaacgacc tggcaagaat
     5161 gaccccagcc ggccgcggga aggcgactac gactacaagc gcgagttcta tcggagttcg
     5221 tcggtcgccg aggactacga cttccaccgt ttcagctctc cggagcgcca gaagcgcaac
     5281 cggcgcaagt gggccgctat ctccagggcg ctgcgcgaag ccaccggcgt tcgaacgatc
     5341 ctcgatctgc cctgcggaac gggaaggttt accggcgcgc tggcgcgcga agggttcgaa
     5401 atcgtcggca gcgacatctc gatggagatg ctgcacaagg cggcttccct tggaaacgaa
     5461 aacaaggcgg atggtcagca tccagcgatc cgcggttacg tgcaggccaa cgccgaacac
     5521 ctgccgctgc gcaacgattc actcgactgc gtcgtcagca tccgcttcat gatgcacgtc
     5581 gacccggtca cgcgcgtgcg catgctgcgc gagttccatc gcgtctcgcg tcgctgggtc
     5641 gtcatcgact accgccactg ttactcgttt cgttacgtgc tcacgcacac gctcggcagg
     5701 ctcggcatcg gccggccgcc gctgtcgcgt gtgtctcgca aggaactcga aaaggagttt
     5761 gccgatgcgg ggttcgcgat ccgcaacgtg attcgcgtgt cggcgcctct tctctcggac
     5821 aagtggatag tcctcgccga gcgggcgtga gcccggttac atcagacgca gttcggcggc
     5881 ggcagcttcg agcccggtca cgtattccct cgtcgagcga agcccgtcct tcaggccgcg
     5941 gtagttcacc agctccagtg cttcctcgta cggacaccag cgcgactccg cgtgttcctg
     6001 cggatcgagc ctgagttcag tcttgccggc ttccgctgcg tataccgcga tcatctgcat
     6061 ggtcccgaaa cggcggttat agatgttgta aacgtgctcg gcagcccaga tgcccgtagc
     6121 ggcgattccg aagtccgcca gcagacgcac gatggccggc accacgcgct ccgaatcctg
     6181 catgaagccg ctcgggatct gccagaaccg cccgccgttg aagtagcggt cggccttgag
     6241 ctgcgaggtg tgcagtagca ggtattccac ctgcgcgggg gtccggcgga acacccagac
     6301 gtcgaaaaca ttggattcga gcttcatgcg cggacgatgc catacgccgg ggccatgaac
     6361 cgcaaatgtc caccgagaca acgactcgcc aggacgagtt ttcccgcatt cgggttgccc
     6421 ccgccagccc gcctgtcgga caatgcgcgc cctttatgga taccccagcc atgaccccca
     6481 cccgccgcgc cctggccaac gccatccgcg cgctctccat ggatgccgtt caggccgcga
     6541 attcgggaca ccccggcgcc cccatgggca tggccgacat cgccgaagtg ctgtggcgcg
     6601 atgtgctcaa gcacaacccc ggcaacccca aatggtggaa ccgcgaccgc ttcgtgcttt
     6661 cgaacggtca cggctcgatg ctgctgtatt cgctgctcca tctgaccggc tatccgctgg
     6721 ccatcgatga actgaagaaa ttccgccagc tgggtgcgcg caccgcggga catcctgaac
     6781 gcgatctcga gatcggtatc gaaacgacga ccgggccgct cggccagggt ttcgcgaacg
     6841 cggtgggcat ggcgctcgcc gagaaacttc tcgcggcgca atacaacaag gacggcgcgg
     6901 cgatcgtcga tcaccacact tactgcttcg tcggtgacgg ctgcctcatg gaaggcattt
     6961 cgcacgaagc ggcgtcgttc gccgggcacc tgaagctcgg caagctcatc gtgttctacg
     7021 acgacaacgg catctccatc gacggcaagg tcgccggctg gttcacggaa gacacgccga
     7081 agcgcttcga agcgtacggc tggcatgtcg tgaagaacgt cgatgggcac gatgccgatg
     7141 cagttgccgc cgcgatcaag aaagcgaaga accgcaagga ccagccttcg atcatctgct
     7201 gcaagactac tattggctgg ggtgcgccta acaagcaggg caccaagtcg gcacacggcg
     7261 aggccctggg tcccgatgaa gtcgcgaatg cgcgcaagac gctgggctgg gaatcgccgc
     7321 ccttcgtcat tcccgaagac atccgggccg cctgggatca tcgcgaggct ggcgccgccg
     7381 cagaaaagaa atggctgcgc gtgtggcgcc gctacaagcg cgaatttccg aaggaaggcg
     7441 cggagttcga acgccgcatg aagggtgatc tgccggccga ctgggcggag atcgtccagg
     7501 ccgcgattgc cgcaggcgcc gctgtcaccg gtgcgcaggc gacgcgcgca tcgtcgcagg
     7561 cggtgctgaa cgtgctcggg ccgaagatgc ccgaaatgtt gggcggctcg gcggatctca
     7621 ccggctcggt gaataccaag cgcaaggatt cggtcgacct cactaccgaa aacttccacg
     7681 gcaactacgt ttattacggc gtgcgcgaat tcggcatgac ggccatcatg aacggcatca
     7741 ccgtgcacgg cggttatcgt ccgtacggcg gcacgttcct ggtgttctcc gattacgcgc
     7801 gcaacgcggt gcgcgtcgcg gcgatcatgc attgcccgac cacgctcgtt tacacgcacg
     7861 attccatcgg tctcggcgaa gacgggccga cgcaccagcc catcgaacat ctcgcttcgt
     7921 tgcgcgccat gcccaacctg cacacctggc gtccctgcga cgcagtggaa tcggctgtcg
     7981 catggacgtc cgcgctcgag cgcaagaatg gcccgacggc gctcatcttc acgcgccagg
     8041 gtctcccgca gcaggcgcgc acccccgagc agttagtcgc gctgcgcaag ggcggctacg
     8101 tactcatcga cagcaacggg ccgccggaag ccatcgtgat cgccacgggt tccgaggtcg
     8161 ggttcgccgc cgaagcggtc aaggaactgc aggctgcggg gaagcgcgtg cgcctggtct
     8221 ccatgccgtg ttgtgaggcc ttcgatgcgc agcccgcgcc ctatcgcgaa agcgtgttgc
     8281 cggcctcgtg catgcgccgc ctcgccgtcg aagcgggcgc cacgcagtcg tggtggcgtt
     8341 acgtcgggtc gcagggccgc gtgatgggca tcgagagctt tggtgcctcg ggcaaaggcc
     8401 cggatctttt caaacatttc ggtctgactg ccgccgatct caaggcgcag gtcgagcaaa
     8461 tgctcgcctg aagaccgcat cagctgcttt cgtaattctt tcgatttcaa cgcaagggga
     8521 tttggacaga tggcaatcaa agtaggcatc aacggttacg gacgtatcgg ccgcaacatt
     8581 ctgcgcgcgc tctatgaagc caagcgcaac aacgaaatcc agatcgtcgc ggtcaatgac
     8641 ctgggcgatg cgcagaccaa cgcgcacctc acgcagtacg acaccgcgca cggcaaattc
     8701 cccggcacgg tgagcgtcga aggcgactcc atcgtcgtga acggcgacaa gatcaaggtc
     8761 ttcgcgcagc gggatccgtc gaaaatcgac tgggcctcgg tgggtgccga atacgtgttc
     8821 gagtccaccg gtttcttcac caccaaggcc gatgcgggca agcatctcgc cggcggtgcg
     8881 aagaaagtcg tcatttccgc cccgggcggc aaggacgtgg acgcgacggt cgtctacggc
     8941 gtgaaccaca acgtgctcaa ggcctcgcac acggtcatct ccaatgcctc gtgcacgacc
     9001 aactgcctcg cgccgttggc caaggtgctc aacgacaaga tcggcatcgt cagtggattg
     9061 atgacgacga tccacgcgta caccaacgac cagcgcctga ccgacgtcta tcacgaggac
     9121 atccgccgtg cgcgttccgc cacgatgtcg atgatcccgg cgaagaccgg cgcggccgcg
     9181 gccgtcggcc tggtgctgcc cgaactcaag ggcaagctcg atggcttcgc gatccgcatt
     9241 ccgaccatca atgtgtcgct ggtcgacctg accttcgtcg ccaagcgcgc cacgaccgtg
     9301 gaagagatca acaaggttgt gaaggaagcc gcggacggcg ccatgaaggg cgtgctcgcg
     9361 tacaccgaca agcagctcgt ctcgatcgac tacaaccacg atccggcatc gtccacctac
     9421 gactcgacgc tgacgcgcgt caccgaaggc acgctggtca aggtctgctc ctggtacgac
     9481 aacgaatggg gcttctcgaa ccgcatgctg gataccacga tcgcctggtc gaaagcctcc
     9541 tgaggccgta atccatgaac gtgctgcgca tggcggatca ggacctcgct ggcaagcggg
     9601 tcctgattcg cgaagatctg aacgtgcccg tcgagggcgg caaggtcacc agcgatgcgc
     9661 gcatccgcgc gtcgctgccg accatcaagc tggcgctcga gaaaggcgcg aaggtgttca
     9721 tctcttcgca tctcggtcgg ccggaagagg gcgtgtacgc ggaagagttt tcgctcgcgc
     9781 ccgtggctgc gcgcctgtct gacttgctag gcaagccggt gctgctcaag aaggactggc
     9841 tcctcggcgt cgacacgcca gcaggttcgg cggtgctgct cgagaacgtg cgcttcaaca
     9901 agggcgagaa gaaagacaag gaagagctcg cgaggcagat ggcgtcgctg tgcgacatct
     9961 atgtcatgga cgctttcggc acgtcacatc gcgccgaagc atccacgcat ggcgtcggca
    10021 agtacgcgaa ggtcgcttgc gcgggcccgc tgctcgtggg cgagctcgaa gcgctcgagc
    10081 gcgcgctggc gaagccggca cggccgctcg tggcgatcgt cgcgggctcg aaggtttcca
    10141 ccaagctcac ggtgctcgaa tccttgttag ccaaggtgga caagctgatc gtgggcggcg
    10201 gcatcgccaa cacgttcctc gctgcgacgg gcatcaacgt gggcaaatcg ctgcacgagg
    10261 cggacatgct cgacatggcg cgcaagctgc tcgatgcggc acgcgcgcgc ggcgcggaaa
    10321 tcccgttgcc tacggacgtg gtggtggcga aggaattcgc ggccacggcg cacgccgaca
    10381 tccgcagcat ccatgaggtt cggcccgacg agatgattct cgacatcggc ccggattccg
    10441 cggagcggtt cggaaagatg ttacagaccg ccggcaccat cgtctggaac ggccccgtgg
    10501 gcgtgttcga atgggatcaa tttggtgaag gcaccaaggc catcgcgcag gccattgcca
    10561 ggagcccggc gttttcgctg gccggtggcg gcgacacgct ggcggccatc gagaagtacg
    10621 gcgtcgagga agacatctcg tacatcagca cgggcggcgg cgcgttcctc gagttcgtcg
    10681 agggaaagac gcttcctgct gtcgcgatgc tcgaatcgcg ggcgaagcaa tagcggcaat
    10741 gaaaacgggg tcgcgcgcag atttcggcac ggcccgtttt tcttcgctcc gttcttccct
    10801 gttgttgttc gcagcc
//
LOCUS       DASQJS010000470         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_2198023, whole genome shotgun sequence.
ACCESSION   DASQJS010000470 DASQJS010000000
VERSION     DASQJS010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198023"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1939)
                     /locus_tag="VEW08_18520"
     CDS             complement(<1..1939)
                     /locus_tag="VEW08_18520"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HYJ42791.1"
                     /translation="MSTAEQLGSLNAPQRKAVTYGEPLPGGKGFRAGPLLIVAGAGTG
                     KTNTLAHRVAHLAMSGVDPARILMLTFTRRASIEMKRRANEIMREAFDEKLGGGKAQA
                     ISQRLTWAGTFHSIGNRLLRHYAKHVGLDPSFSVLDRSDSADLVDTVRTELGYSGKGQ
                     RFPRKDTCLSIYSYRVNTQKSLKETLETQHPWCAHWEEDIAKLLRAYVERKSQNRVLD
                     FDDLLLYWHAMMSEDRIAKSVSAHFDHVLVDEYQDTNKLQGDILAALKPDGQGVTVVG
                     DDAQAIYSFRAAAVENILGFPDRYRPKAEVVTLAQNFRSSQSILDSANALMAEAPRQY
                     RKHLLSMRGQGPRPKLVTVEDLPTQAEFICTRVLASREAGIPLKRQAVLFRTGTHSDV
                     LEIELAKRKIPFVKYGGLKFLDAAHVKDLLAVLRWADNPHNQLAAFRVLQLMPGMGPA
                     NAKRALDHFVASGHLWTSFEKFEPPTEARAAWKKFNELLAALGDPNREWRGQIHLVRE
                     WYRPHMERLYDHVHTRIGDLDQLELLSSQYQNRERFCTELTLDPPHATSDLAGQPVMD
                     EDYLVISTVHSAKGMEWDNVFILNVVDGSFPNEFATKAELIEEERRVLYVAMTRARNE
                     LTCIVPLRFQITSQPKTSDGHV"
BASE COUNT          360 a          612 c          682 g          401 t
ORIGIN      
        1 agacatggcc gtccgaagtc ttcggctggc tggtgatctg gaagcgcaac ggcacgatgc
       61 aggtgagctc gttgcgcgca cgggtcatgg ccacatacag cacccggcgc tcttcttcga
      121 tgagctcggc cttggtggca aattcgtttg gaaaactgcc gtcgacgaca ttgaggatga
      181 agacgttgtc ccactccatg cccttggcgg agtgcacggt ggagatcacg aggtagtcct
      241 cgtccatcac cggctggcca gccaggtcgc tggtcgcgtg cggcggatcg agcgtgagct
      301 cggtgcagaa tcgttcgcgg ttctggtact gcgaagacag cagctcgagc tggtcgaggt
      361 caccgatacg cgtgtgcacg tgatcgtaca gacgctccat gtgcggccgg taccactcgc
      421 gaaccaggtg gatctggccg cgccattcgc gattcggatc gcccagggcc gcaagcagct
      481 cgttgaattt cttccaggcg gcgcgcgcct ctgtcggggg ttcgaacttt tcgaacgagg
      541 tccacagatg gccgctggca acgaagtgat cgagggcgcg cttggcattg gccgggccca
      601 tgccaggcat gagttgcagc acgcggaagg cggccagctg attgtgcggg ttgtcggccc
      661 agcgcagcac cgccagcagg tccttgacgt gtgcggcgtc gaggaacttg aggccaccgt
      721 acttcacgaa aggaatcttg cgtttcgcca gctcgatttc gaggacgtcg ctgtgagtgc
      781 cggtgcggaa caggaccgcc tggcgcttga gcgggatgcc ggcttcgcgc gaggccagga
      841 cccgggtgca gatgaactcc gcctgcgtcg gcagatcttc gactgtcacg agcttcggcc
      901 gcggaccctg tccgcgcatc gacagcagat gcttgcggta ctgccgcggc gcctccgcca
      961 tgagggcatt cgcggagtcg aggatgctct ggctcgaacg gaagttctgc gccagcgtga
     1021 ccacctcggc cttgggtcgg tagcgatccg ggaagccgag aatattctcc acggcagcgg
     1081 cacggaatga gtagatagcc tgtgcatcgt cgccgaccac cgtgacgccc tgcccatccg
     1141 gcttgagcgc ggcgaggatg tcgccctgca gcttgttcgt gtcctggtat tcgtcgacca
     1201 gcacgtggtc gaaatgcgcg gacacggact tggcgatccg gtcttcgctc atcattgcgt
     1261 gccagtagag cagcaggtcg tcgaaatcga gtacccggtt ctgtgacttg cgttccacgt
     1321 aggcgcgcag cagcttggcg atgtcctctt cccagtgcgc gcaccaggga tgctgcgtct
     1381 cgagcgtttc cttgagcgac ttctgcgtat tcacgcggta ggaatagatc gagaggcagg
     1441 tgtccttgcg cgggaaccgc tggcccttgc ccgaataacc gagctcggtg cgaaccgtgt
     1501 cgaccaggtc cgccgaatcg gagcgatcga ggacactgaa agatggatcg aggcccacgt
     1561 gcttggcgta gtggcgcagc aggcgattgc cgatggaatg gaacgtgccg gcccaggtga
     1621 gccgttgcga aatcgcctgc gctttgccgc cgccgagctt ctcgtcgaaa gcttcgcgca
     1681 tgatttcgtt ggcgcggcgc ttcatctcga tggaggcgcg ccgcgtgaac gtcagcatca
     1741 ggatgcgcgc gggatcgacg ccactcatcg cgaggtgcgc cacgcgatgc gcgagcgtgt
     1801 tggtcttgcc agtgcccgcg ccggcgacga tcagcagcgg acccgcgcga aagcccttcc
     1861 caccgggaag aggctcaccg taagtcaccg ccttgcgttg cggagcgttc agacttccga
     1921 gttgttcggc cgtggacatc aatacaacaa tatatgaatg gcgattgaaa ttaggaccgg
     1981 ctccccggaa gccgtgacgg gcgtcacgcc ggtatctggc cggaatgtga cgcctttttg
     2041 atctcgattt gcccc
//
LOCUS       DASQJS010000471         3086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_1256122, whole genome shotgun sequence.
ACCESSION   DASQJS010000471 DASQJS010000000
VERSION     DASQJS010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256122"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VEW08_18525"
     CDS             <1..669
                     /locus_tag="VEW08_18525"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ42792.1"
                     /translation="FYKDVDSFVQTLRDQRPFTGNPYGLDDSVAIAACGNTAGCSPAS
                     IWDFNTPQTTEGGPVKGFEISYQQPFSFLPGVWSNFGAILNYTGVESEITYITVSGAV
                     GAPPVYTPVTDDLTGLSKDAYNATLYFDNKVFSARVSAAYRSDYLTTVPGRNSNDVEG
                     TAETMNIDFSTSYNINDNFSVSLEALNLTDEVQDQWVGTAADRLSYYHHQGTQYYLGA
                     RFKY"
     gene            785..1762
                     /locus_tag="VEW08_18530"
     CDS             785..1762
                     /locus_tag="VEW08_18530"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYJ42793.1"
                     /translation="MNRREIILAGTMAALTSASRTPAAPEPGDSVIELWPGGAPGGDK
                     VTVKEEIVERLPDGPLRDRFVQHVTRPLLTLFKPRTAYNGITLLIVPGGGYVRVVIDK
                     EGVEAAEWFTQRGFAAAVLRYRLPADGWANGPDAPVQDAMRAMRLLRANAAKHGTPMR
                     SIGVIGFSAGGHLCARLLTEPGITYARRDSADDLSARPDFGVLMYPVIAMTGPYAHAG
                     SVSQLLAAGHGSGSESGSPVNPDGFAPHFHVGRNTPPTMLVHAADDTSVPLENSLLMY
                     DALRKAGVRSELHAFDSGGHGFGLRSVAGKDVAAWPTLVQNWATNNATP"
     gene            1749..>3086
                     /locus_tag="VEW08_18535"
     CDS             1749..>3086
                     /locus_tag="VEW08_18535"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HYJ42794.1"
                     /translation="MRHPEIVDRRRRRLLGVGAASVGLAMSPFARAATAGPIVRTTAG
                     RVRGTRDGDLQVFRGIRYGADTRSRRFMPPVAPAPWRGIVEATAFGAASMQPGSEANQ
                     SEDCLFLNVTAPLEKSTRPRPVIVYIHGGAYSSGSGSSPLYDGANLSRRGDVVVVTLN
                     HRLSLFGYLYLKEFPDSGNAGMLDLVLALQWVRDNIAAFGGDPGCVTLLGQSGGGAKI
                     ATLMAMPVAAGLFHRAATMSGQQLTASGPLHAAGRTRVLLDKLGVPVERAATVASLTA
                     AELLRAHSGSVDPYIGRGSVYMGPVLDERTLTRHPFYPDAPPQSAGIPMMIGNTHDET
                     RSLIGRGDPGVFAITWEALPQRLEAELRVDISAALVVAEYRRWYPHYSPADVFFSATT
                     AGRSWRAAIVEAELRAAQGSPAFAYQLDWGSPQDGGKWGAFHTLDIPLMFGTLT"
BASE COUNT          531 a         1015 c          979 g          561 t
ORIGIN      
        1 ttctacaagg atgtcgacag cttcgtgcag acgctgcgag atcagcgtcc gttcaccggc
       61 aatccctacg gtctcgacga cagcgtcgcg atcgcggcct gcggcaatac cgccggttgc
      121 agcccggcat cgatctggga cttcaacact ccgcaaacca cggaaggcgg cccggtcaaa
      181 ggcttcgaaa tcagctacca gcagccgttc agcttcctgc ccggcgtgtg gagcaacttc
      241 ggcgccatcc tcaactacac cggtgtcgaa tccgagatca cgtacatcac ggtatcgggc
      301 gcggtgggcg cgcctcctgt ctacaccccg gtcaccgacg acctcacggg tctgtcgaag
      361 gacgcctaca acgccacgct gtacttcgac aacaaggtct tcagtgcgcg cgtgtcggcg
      421 gcctaccgct ccgactacct cacgacggtg ccgggtcgta acagcaacga tgtcgaaggc
      481 acggcggaga cgatgaacat cgacttctcg acctcgtaca acatcaacga caacttctcc
      541 gtctcgctcg aggcgttgaa tctcaccgat gaagtgcagg accagtgggt gggaacggct
      601 gccgatcgcc tgtcctacta tcaccaccag ggaacgcagt attacctggg tgcccgcttc
      661 aagtactagt tagatcgggg aacgccccgg actcgatgcg atccaatggg gacagaagaa
      721 attctgtccc catttttgtt tgcggcggca tactccgccc atgaagccgc cccaaaagtt
      781 gtcaatgaat cggcgagaga tcattctcgc tggcaccatg gctgccctca cctccgcgtc
      841 gcgtacacct gctgctcccg agcccggtga ttcggtcatc gagctctggc cgggcggcgc
      901 gcccggcggc gacaaagtca cggtcaaaga agaaatcgtg gagcggctgc cggatggccc
      961 gctgcgcgat cggttcgtgc agcacgtgac gcgcccgctg ctgaccctgt tcaagcccag
     1021 gaccgcctac aacggcatca cgctgctcat cgtgcccggt ggcggctacg tgcgcgtggt
     1081 catcgacaag gaaggtgtcg aggcagccga atggtttacg cagcgcggtt tcgccgctgc
     1141 cgttttgcgt tatcggctgc ctgcggatgg gtgggcgaac ggccccgacg caccggtgca
     1201 agacgcgatg cgcgccatgc gcctgcttcg cgcgaatgcg gcgaaacacg ggacaccgat
     1261 gcgaagcatc ggcgtcatcg ggttctcggc gggcggccac ctgtgcgccc gccttcttac
     1321 cgaaccaggc attacctacg cacggcgtga ttctgcggac gatctgtcgg cgcgtccgga
     1381 tttcggtgtg ctcatgtatc cggtgatcgc gatgacgggt ccatatgcgc acgcggggtc
     1441 cgtaagccag ttgctggccg caggccatgg ctcggggtcg gagtcgggca gtcccgtgaa
     1501 tcccgatggt ttcgcgcctc actttcatgt cggacgaaac actccgccca ccatgctggt
     1561 gcacgcggcg gatgacacca gcgttccact ggaaaactcg ttgctgatgt acgacgcctt
     1621 gcgcaaggcg ggtgtgcgca gcgaattgca cgctttcgac agcggcggac acggctttgg
     1681 gctgcgtagc gtcgccggca aggacgtggc cgcgtggccg actctggttc agaactgggc
     1741 gacgaacaat gcgacaccct gaaatcgtcg accgccgtcg gcgcagactg ctgggcgtgg
     1801 gagcggcgtc ggttggcctg gcgatgtccc cattcgcgcg cgcggcaacg gccgggccga
     1861 tcgttcgaac cacggccgga cgagtgcgcg gcactcgcga cggtgacctg caggtattcc
     1921 gcggcatccg ttacggcgcc gataccaggt cacgccgctt catgccgccg gttgcgcccg
     1981 cgccctggcg cggaattgtc gaagccacgg ccttcggcgc ggccagcatg cagccgggtt
     2041 cggaagcgaa tcagtccgaa gactgcctgt tcctcaatgt gacggcgccg ctcgagaaat
     2101 ccacgcgccc gcgaccggtc atcgtgtaca tccacggcgg cgcttattcg agcggctccg
     2161 gctcgagccc cctgtacgac ggcgccaacc tgagccgtcg cggtgatgtc gtcgtggtga
     2221 cactgaacca tcggctcagc ttgtttgggt atctttattt gaaggagttt ccggattccg
     2281 gcaacgctgg aatgctcgac ctggtgctgg cgctgcaatg ggtgcgcgac aacatcgcgg
     2341 cgttcggcgg tgatcccggt tgcgtgactt tgttaggcca gtcgggcggc ggcgcgaaaa
     2401 tcgcgacgct catggcgatg ccggtggccg ccgggttgtt tcatcgcgca gccacgatga
     2461 gcggccagca actgaccgcc tccggcccgc tgcatgcggc cgggcgcacg cgcgtgctgc
     2521 tggacaaact cggcgtcccg gtggaacgcg cggcgacagt cgctagcctc accgcggcgg
     2581 aactgttacg cgcgcattcc ggatccgtcg acccgtacat cgggcgcggc agcgtctaca
     2641 tgggaccggt actcgacgag cgcactctca cgcgccatcc gttttatccg gacgcgccgc
     2701 cgcagtcggc gggcataccg atgatgatcg gcaacaccca tgacgagacc cgctcgctca
     2761 tcggacgcgg cgatcccggc gttttcgcca tcacctggga agcgctgccg caacggctcg
     2821 aggcggaatt gcgcgtcgac atttccgcag cgctggtcgt cgccgaatat cgcaggtggt
     2881 atccgcatta ttcccccgcc gacgtcttct tctccgcgac caccgcagga cgttcctggc
     2941 gcgcagccat cgtcgaggcg gaactgcgcg ccgcgcaggg ctccccggcg ttcgcctatc
     3001 agctcgactg gggctcgccg caggacggtg gcaagtgggg cgcgttccac acgctggaca
     3061 tcccgctgat gttcggcact ttgacc
//
LOCUS       DASQJS010000472        12177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_3768506, whole genome shotgun sequence.
ACCESSION   DASQJS010000472 DASQJS010000000
VERSION     DASQJS010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12177
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3768506"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VEW08_18540"
     CDS             complement(<1..560)
                     /locus_tag="VEW08_18540"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYJ42795.1"
                     /translation="MINQTRIAILALLIWQMPCLAQSPNAFGIPTAGNPQPASTINPV
                     PGSRAQGWPAQGRSEVLARHGIVATSDPLAAQAGLEILQQGGNAVDAAVATGAVLDVT
                     SPNDTGIGGDLFALVYIARDKKLYALNSAGWAPAQWTPEYFTKKLGVKAMPPGGVNAT
                     TVPGAISGYDALLKRFGTMTFKQTFDR"
     gene            complement(566..1258)
                     /locus_tag="VEW08_18545"
     CDS             complement(566..1258)
                     /locus_tag="VEW08_18545"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HYJ42796.1"
                     /translation="MPEEASSVSQRIRRRLEAKQQRFHANDNISAFIEPGELEELTKE
                     VAAKMAGVLEALVIDVEHDHNTRETARRVAKMYVNEVFRGRFVPMPNVTEFPNAAHLN
                     ELMIMGPITVRSACSHHLCPIMGRLWIGVMPNQDSALIGLSKYSRLAEWIMTRPQIQE
                     EAVIQLANMLEEKVEPDGVAVVMEADHFCMHWRGVKDNASKMTNSVMRGVFLKDGALR
                     REFLSLISLKSS"
     gene            1385..2491
                     /locus_tag="VEW08_18550"
     CDS             1385..2491
                     /locus_tag="VEW08_18550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYJ42797.1"
                     /translation="MPQNRTKGTPERATTDKSLTKERQNADQALAEQKAAVEADADRV
                     VKLAREQADTVLHAARDKADVKLESVMPTPLVRAVIERERKSEDRAVENERAAADEAL
                     QQEREQNARDLLALLPLERKSTDRSLSTERVRSDDAVAHRDDVLAMVSHDLRDLLGAI
                     VSSSALVLRTAAVGDAGNTTRTGAERIQRYAARMKRLIDDLTDVARIDAGKLSLRPAP
                     GNLAPLINEAVLALRGAATSKDLSLDLPFCDEPIPVIFDGDRVLQVLTNLIANAIKFT
                     PAGGRIRVECAHAEHMARVSISDTGPGIAPDLLEAIFERFRQGENQERRGLGLGLYIS
                     RTLIEAQHGKIWAESTPGNGTTVSFTLPLMREVT"
     gene            complement(2576..3724)
                     /locus_tag="VEW08_18555"
     CDS             complement(2576..3724)
                     /locus_tag="VEW08_18555"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYJ42798.1"
                     /translation="MNNPVRGLRNCLLATALGLSSLAAGAADDPGLISQGKEIFRFDT
                     FGDEQLWTDKLGLHKVVEQKVDPTTALKVGLKVDSEVLPPGILEKVDLKDPKTTVALL
                     KMNAVVGLKAEVDANNHITRLGVTCALCHSTVDDSVQAGIGKRRDGWPNRDLDVGKII
                     SLSPALTAEQKKVYTSWGRGKYDPRYNQDGKNNPLVIPPAYGLAKVKNETYTAEGPIS
                     YWNAYVAVTQMGGQGNFKDDRLKIDVKHSPDLVTSKLPALRAYQHSLPAPRADASSYD
                     AQAAQRGKVTFNRTCATCHVGGSGTDNDSGKLHAPADTGMDSAYAMRTANRAFRTTPL
                     RGLQHHPPYFHDGSAKTLGDVVEHYNKERSLSLTKDQLGDLVEYLKSL"
     gene            complement(3871..4554)
                     /locus_tag="VEW08_18560"
     CDS             complement(3871..4554)
                     /locus_tag="VEW08_18560"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYJ42799.1"
                     /translation="MTISRMTLYSGPLSMFGAKVQIAALEKGLDFDLIMVPYDSARGY
                     DPKHPEVLRINPKRQVPVLTHGPVEIFDSTQIFEYLEDMAPTPALWPRDPIARAAARN
                     LEHQSDEVYFPHIIKLMGLQDALATDPAVAAIEGANRFYAAMERRLEADWLAGSYSFA
                     DIAFYMAALFGERQGAPLTAGTPRLLAWRERMGQRAPVISVVSAMAAWLKSAGRPVPA
                     FMFALQTSK"
     gene            complement(4551..5192)
                     /locus_tag="VEW08_18565"
     CDS             complement(4551..5192)
                     /locus_tag="VEW08_18565"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HYJ42800.1"
                     /translation="MKQRGPKFSRFPGPAPRIRSFATSKALETWMRAHHAKAPELWLK
                     IHKKDSGLRSVTHAEALDVALCWGWIDGLRKSFDERSFLQRFSPRKAKSIWSQINREN
                     VARLNEAGRMTPHGQKQVDAAKADGRWDAAYAPIRSTSHDTMPADLLAAIKANPKAQK
                     KFESLGRVNLFSLRFRTNNMKTVAGREKKIATLVAMLARGETIVPEKTRGRKR"
     gene            complement(5225..6406)
                     /locus_tag="VEW08_18570"
     CDS             complement(5225..6406)
                     /locus_tag="VEW08_18570"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYJ42801.1"
                     /translation="MKPGNAVAIALCMAVAVLEGFDIQAMGVAMPRLGPEFGLDADAK
                     GWLFSVNNIGMVLGAAVGGWLADRTGRRPVLLSAVALFGLFTFWVAIAKSYDALVTAR
                     FLAGLGFGAALPNMMAIAAELSSPARRALTASLVFCGLPIGGGTVALLTQVFPPDFDW
                     RTWFIIGGVLPMVLLPGLRWLLPETLVPEQRQGPKDRTPLLEALFGGGRTMPTLLLWL
                     TFLPTLLILYLMLNWLPTLVVAKGLDRAVAPQASLAFNWASVAGALAAGWLVDRFGPR
                     WPLTLAYAGLIASLVALGAATNHTMIIAYSGIAGFLLLGANYALYGVAPSYYPTIMRG
                     TGSGASVGVGRVGSILGPLAAGIMLSGGQSAVDVVQYMAPVAAVAGIAVFALSFFRRE
                     H"
     gene            complement(6416..7606)
                     /gene="pobA"
                     /locus_tag="VEW08_18575"
     CDS             complement(6416..7606)
                     /gene="pobA"
                     /locus_tag="VEW08_18575"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HYJ42802.1"
                     /translation="MRRTQVAIVGAGPAGLLLGQLLYKEGIETILLERRSAEYVSGRI
                     RAGVLEPGTVNVLERAGVASRLHAEGLLHEGVEIGFRGRRHRIDLKALTGKSVTVYGQ
                     TEVTRDLMDARAAAGAPSVYEAEDVALHDIAGPSPRVTYRHRGVGHEVACDFVAGCDG
                     FHGISRRSIPAAALSVYERVYPFGWLGVLADFPPPWEELIYANSEHGFALASMRSPTR
                     VRCYLQCSLEDRIGQWSDERFWTEFGRRLGSEIGARVPSTPIAPSIEKSIAPLRSFVA
                     EPLRFGRLFLAGDAAHIVPPTGAKGLNLAVSDVFYLSEALRDFYREPPGDRAESALDA
                     YSARALRRIWHAERFSWWMTRQLHRFPGDAFDERLQHAELEHLFESRAAQASMAENYV
                     GLAL"
     gene            complement(7609..9933)
                     /locus_tag="VEW08_18580"
     CDS             complement(7609..9933)
                     /locus_tag="VEW08_18580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYJ42803.1"
                     /translation="MKTKPQAKPLAMAVTQAIAMAAATAYAPSAIAQAPEVQRLEEII
                     VTARKVEEDLQKTPIAITALSGDSLEERQVFRTDVLDQVVPNLQFANNAPLAGNNSSS
                     QVFIRGIGQTDPTSTVDPGVGLYIDDVYIGNAVGASMALRDVANVQVLRGPQGTLFGR
                     NTIGGAVLISTTDPGDDFGGEVRAELGNDSLADGQVSLDIPFGEKFKTRLSLGMRKQD
                     GYVTRPDGTDLGDTDTFFGMFKGVWTPSERVRATFIVDVTSSDENGSPLVFATINEAA
                     TFPRVASLDAGCPGLTDGAVPPPGLPDQPQVPMISDPRCANDLQGAGPYSNNGTLPLY
                     STLDNVGASFNIAFDLSESITLKSITAYRKSDWTGARDADNTPLTILHTEYDVSGDQL
                     SEELQFVYETDPLVGVVGLYYFEQTSDDIATIALNPPPPGVQRDSDNNEVDNSSWAAF
                     TQWTYNFAGKFALTVGGRYTEDDKGSYPDQFDYATPNIKQVPVQWYRETFTSFTPSAA
                     FSVEWNESAMTYLSYSEGFKGGGWNSHFNAVLTPTQQAALQKFDQEEAQTIELGAKFD
                     LANDTMRLNLAVFSSDYTNMQLTYRGPAPNGVAPFITNAGETSIDGAEAEFTWAATDN
                     LLIDASVGYLDATIDSLSNIPLAVLPPGLVEGNFLPFAPEWQAHLGIAYTGHAGSLEI
                     KPRVDASFQPTTFFDATNTPEIAQLDDVLTVNASVAFGGGDDTPWSVTLGVNNLTDEE
                     YPIAGNSSLTTGSGYAEVAYSRPLEWFASLKFEF"
     gene            complement(9953..11068)
                     /locus_tag="VEW08_18585"
     CDS             complement(9953..11068)
                     /locus_tag="VEW08_18585"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HYJ42804.1"
                     /translation="MPLTQPPFRADHVGSLLRPPELTRARAEHKAGRLDAAGLRKAED
                     AAIRDVVQVQEALGLQGITDGEFRRSSWHMDFLYQIGGVRKVQQNITVHFHSDKGDVD
                     FTPSGLKVEGKLSLDGCIFGDHFDFLKSVVKRGTPKLTIPSPSMMHYRGGRAAIDETV
                     YPRMEEFWRDLSAVYAREIAELAKRGCTYLQLDDTSLAYLNDPKQREYVSSIGGDPNE
                     QHRIYIRNINAALAGKPAGMRVCTHMCRGNFRSAWAAEGGYDHVAEALFNELAVDGFF
                     LEYDDARSGGFEPLRFVPKGKFVVLGLVTTKRGQLETKDDLKRRIDEAARFVPLEQLC
                     LSPQCGFSSTEEGNALSLDEQKAKLALVIETAREVWK"
     gene            complement(11059..11733)
                     /locus_tag="VEW08_18590"
     CDS             complement(11059..11733)
                     /locus_tag="VEW08_18590"
                     /inference="COORDINATES: protein motif:HMM:NF040065.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42805.1"
                     /translation="MEIPLADSLKDAIAVAELAQRTRLVCMVGHTRRFNPSHQWIHRR
                     IEAGELAVQQMDVQTYFFRRSNMNALGQPRSWTDHLLWHHAAHTVDLFRYQARSEIVR
                     ANVVQGPIHPVLGIAMDMSIQLQAASGAICTLSLSFNNDGPLGTFFRYICDHGTYIAR
                     YDELVDGKNNAIDVSKVDVSLNGIELQDREFIAAIRDGREPNSSVAQVLPCYRTLHEL
                     EQQLCR"
     gene            complement(11734..12012)
                     /locus_tag="VEW08_18595"
     CDS             complement(11734..12012)
                     /locus_tag="VEW08_18595"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYJ42806.1"
                     /translation="MKAMKVVVAGVGAFGQKHLDACKLIEGVEVVSVVGRQLAPTQEV
                     ARKYGVAHATTDLAEALARPGVDAVILATPTQMHAAQSVQCLNAGKHV"
BASE COUNT         2177 a         3886 c         3868 g         2246 t
ORIGIN      
        1 cgatcgaacg tctgcttgaa ggtcatcgtg ccgaagcgtt tgagcaacgc gtcgtaaccc
       61 gaaatcgcgc ccggcaccgt cgtggcattg acaccgcccg gcggcatcgc cttgacgcct
      121 aacttcttcg tgaagtattc gggcgtccat tgcgccggcg cccagcccgc ggagttgagc
      181 gcgtagagct tcttgtccct ggcgatatac accagcgcaa ataggtcgcc gccgatcccg
      241 gtgtcgttgg gcgaggtgac atcgagcacc gcgccggtcg ccaccgccgc atcgacggcg
      301 ttgccgccct gctgcaggat ttcgaggccg gcctgggccg cgagcgggtc gctggtcgcg
      361 acgatgccgt ggcgcgcgag aacttccgac cggccctgtg ccggccatcc ttgcgcgcgc
      421 gacccgggca cggggttgat ggtgctggct ggctgcggat tgccggctgt gggtatgcca
      481 aaggcgttcg gagattgggc gaggcacggc atctgccaga tcaacagggc caggatggca
      541 attcgggtct ggttgatcat gggcctcagg agctcttgag gctgatgagc gagaggaatt
      601 cgcgccgcag tgcgccgtcc ttgaggaaca cgccgcgcat gacgctgttg gtcatcttcg
      661 atgcgttgtc cttgacgcca cgccagtgca tgcagaagtg atctgcttcc atgactaccg
      721 cgacgccgtc cggctccacc ttctcttcga gcatgttggc gagctggatg accgcctctt
      781 cctggatctg cggccgcgtc atgatccatt cggcgaggcg cgagtacttc gacaggccga
      841 tgagcgcgga gtcctggttc ggcatcacgc cgatccagag gcggcccatg atcggacaca
      901 ggtgatgact gcatgcgctg cgcacggtga tgggccccat gatcatgagc tcgttcaagt
      961 gcgcggcgtt cgggaactcg gtgacattgg gcatgggaac gaagcggccg cggaagactt
     1021 cgttcacgta catcttcgcc acgcggcgcg cggtctcgcg cgtgttgtgg tcgtgctcca
     1081 cgtcgatgac gagcgcttcg agcacacccg ccatcttcgc cgccacctct ttggtcagct
     1141 cctcgagctc gcccggctcg atgaatgcgg agatgttgtc attggcgtga aagcgctgct
     1201 gctttgcttc caggcggcgg cgtatgcgct gggagacgga actcgcttct tctggcatgg
     1261 gagctcaccc gtacgggaca ggcgcatagc atacacgcgc gcatttcaga cgcctgataa
     1321 ggcgattcga ggtacgcgcg agatggccta catgccgcgt caggctcgcg tgatagcgtg
     1381 gtcgatgcct caaaaccgga ccaagggaac gcccgagcgg gcgacgaccg acaagagcct
     1441 caccaaggag cgacagaacg ccgaccaggc tctcgctgaa cagaaggctg ccgtcgaagc
     1501 cgacgccgat cgcgtggtca agctcgcgcg cgaacaagcg gacaccgtgc tgcacgccgc
     1561 tcgagacaag gcagacgtca agctcgaatc cgtcatgcct accccgctcg tccgcgcggt
     1621 catcgagcgg gagcgcaaaa gcgaagaccg cgccgtcgaa aacgagcgag cggcggccga
     1681 cgaggctttg caacaggaac gtgagcagaa tgcacgtgac ctattggcac tgctgccact
     1741 cgagcgcaag agcaccgatc gttccctgtc gaccgagcgc gtgcgctcgg acgacgccgt
     1801 cgctcatcgc gacgatgttc tcgccatggt cagccatgac ctccgcgatt tgttaggggc
     1861 gatcgtcagt tcctccgcac tggtgttgcg cactgccgcc gtcggggatg ccggaaatac
     1921 gacacgcact ggcgcagagc gtatccagcg ttacgccgct cgcatgaagc gactcatcga
     1981 cgacctcacg gacgtggcga gaatcgacgc cggcaaactg tctctgcggc cggcgccggg
     2041 caatctggcg cctctcatca acgaggcggt cctcgcgttg cgcggtgcgg cgacgtcgaa
     2101 agacctttcg ctcgacctgc ctttctgcga cgaacccatc cccgtgatat tcgacggtga
     2161 ccgggtgctg caggtactga ccaacctgat cgccaatgcc atcaagttca cgcccgcggg
     2221 cgggcgcatc cgtgtcgaat gcgcgcatgc ggagcacatg gcgagagtat cgataagtga
     2281 cacgggtccg ggaattgcgc cggacctcct ggaggccatc ttcgagcgct ttcgccaggg
     2341 tgaaaaccag gagcggcgcg gtctgggcct gggtctctac atctcacgca ctctcatcga
     2401 ggcgcagcac ggaaagatct gggcggaaag tacgcctggc aacggcacca ccgtgtcgtt
     2461 cactctgccc ctgatgaggg aagtcaccta gcgacgcgat agtcaggtgc cgggtgggga
     2521 aagtggggag tacgcgactc cccactttcc ccacccggca ccgccaccgc catcgtcaca
     2581 gcgacttcag atactccacg agatcgccaa gctggtcctt ggtgagactc aatgaacgtt
     2641 ccttgttgta gtgctccacc acgtctccga gtgtcttcgc gctgccgtca tggaagtacg
     2701 gcggatggtg ctgcaggcca cgcaatggcg tcgtgcggaa tgcgcgattc gcggtacgca
     2761 tggcataggc gctgtccatg cccgtatcag cgggcgcatg cagctttccg gaatcgttgt
     2821 ccgtaccgct gccacccacg tgacaggtcg cgcaggtgcg gttgaacgtg accttgccgc
     2881 gctgtgcggc ctgggcgtcg tagctcgaag cgtcggcgcg gggtgccggc aggctgtgct
     2941 ggtaggcgcg cagcgccggt agtttggagg tcaccaggtc cggcgagtgc ttgacgtcga
     3001 tcttgagccg gtcatccttg aaattgccct gcccgcccat ctgcgtcacg gccacgtacg
     3061 cattccagta ggaaatcgga ccttcggccg tgtacgtctc gttcttgacc ttcgcgagcc
     3121 catacgcggg cggaatgacc agcggattgt tcttgccgtc ctggttgtag cgcgggtcgt
     3181 atttgccccg gccccacgag gtgtagacct tcttttgctc agcggtgagc gccggcgaca
     3241 gtgagatgat cttgcccacg tcgaggtcgc gattgggcca gccatcgcgg cgcttgccga
     3301 tgccggcctg gaccgaatca tcgacggtgg aatgacacaa cgcgcaggtg accccgaggc
     3361 gcgtaatgtg attgttcgca tccacctcgg ctttgaggcc cacgacggcg ttcatcttca
     3421 gcaatgccac cgtggttttc ggatccttca gatccacctt ctcgaggatg ccaggcggca
     3481 gcacttcgga atcgaccttg agtcccacct tgagcgccgt ggtcgggtcg actttctgct
     3541 cgaccacttt gtgaagcccg agcttgtcgg tccagagctg ctcgtcgcca aaggtgtcga
     3601 agcggaagat ttccttaccc tggctgatga ggccgggatc gtcggcggct cccgccgcca
     3661 ggctcgacaa gcctaacgcg gtcgccaaca aacaatttcg cagtccacgc acggggttgt
     3721 tcatgtcgtc tcctgactcg taggcagttg tcttgcaatt gcaagctacg cctaacttcc
     3781 gggagcgatc tgtaggagtg cggaacgttc aggacagggc tttttctcgc gcaacgacgg
     3841 gtaatgcacg aagcaccaat tttgttcccg ctatttcgat gtctggagag caaacatgaa
     3901 tgccggcacg ggccggccgg cacttttcaa ccacgcagcc atcgcgctga ccacgcttat
     3961 caccggcgca cgttgcccca tgcgctcgcg ccaggcaagc aggcgcggtg tgcccgcggt
     4021 caacggggcg ccctggcgct cgccaaacaa cgcggccatg tagaaagcga tgtccgcgaa
     4081 cgagtagctg ccggcgagcc aatccgcttc cagccgccgc tccatcgcgg cgtagaagcg
     4141 attcgcgcct tcgatggcgg cgaccgcggg gtcggtggcc agcgcatcct gcagacccat
     4201 gagcttgatg atgtgcggga aatacacttc gtcggattga tgctcgagat tgcgcgcggc
     4261 ggcacgcgcg atgggatcgc gcggccagag cgccggcgtc ggcgccatgt cttcgaggta
     4321 ttcgaagatc tgcgtggaat cgaaaatctc gacggggccg tgcgtcagga ccggcacctg
     4381 gcgcttcggg ttgatgcgga gcacttccgg atgcttcggg tcgtaaccgc gtgccgagtc
     4441 atagggaacc atgatcaggt cgaagtcgag gcccttctcg agcgcggcga tctgcacctt
     4501 cgcgccgaac atgctgagcg gcccggaata cagggtcatt cgcgaaatcg tcatcgcttc
     4561 ctgcctcgcg tcttctccgg aacgatggtt tcgccgcgcg caagcatggc caccagcgtg
     4621 gcgatcttct tctcgcgtcc ggccacggtt ttcatgttgt tagtgcgaaa ccggagcgaa
     4681 aacagattca cgcggcccag cgactcgaac ttcttctgcg ctttgggatt ggccttgatc
     4741 gcagcgagca ggtcagcggg catggtgtca tgcgaggtgc tgcgtatcgg cgcataggcg
     4801 gcgtcccatc ggccgtccgc cttggcggca tccacctgtt tctgcccgtg gggcgtcatg
     4861 cggccggctt cgttcagtcg cgcgacattt tcgcggttga tctggctcca gatgctcttt
     4921 gccttgcgcg gcgagaagcg ctgcaggaac gagcgctcgt cgaacgattt gcgcaggccg
     4981 tcgatccaac cccagcagag cgcgacgtcc agcgcttcgg cgtgcgtcac cgaacgcagc
     5041 cccgaatcct tcttgtgaat cttgagccac agctcgggcg ccttggcgtg gtgagcacgc
     5101 atccacgtct ccagcgcctt cgacgtggcg aagctgcgga tgcgcggtgc cgggccggga
     5161 aaccgggaaa actttggtcc acgttgcttc atgaagttat cccggtttcc cggcccggca
     5221 ccgtttagtg ctcgcggcgg aaaaagctca atgcgaacac ggcgatgccg gcgacggccg
     5281 ccacgggcgc catgtactgc accacgtcca cggcgctctg cccgcccgac aacatgatcc
     5341 cggccgcgag cggccccagg atcgatccga ctcggccgac gccaacgctc gctccggagc
     5401 cggtgccacg catgatagtc gggtagtaag acggcgcgac gccgtacagc gcgtagttgg
     5461 cacccagcag caggaagcct gcgatgccgc tgtacgcaat gatcatcgtg tgattggtcg
     5521 ccgcaccgag cgccaccagc gatgcgatga gcccggcgta ggccagggtc agcggccagc
     5581 gcggtccgaa gcggtccacc agccagcctg ccgccagggc accggcaacg ctggcccagt
     5641 tgaacgccag cgaagcctgt ggcgcgacag cgcgatcgag tcccttggca accaccaggg
     5701 tcggcagcca gttcagcatg agatagagaa tgagtaacgt gggcaggaac gtgagccaca
     5761 gcagcaacgt aggcatcgtg cgcccgccac caaacaaggc ttcgagaagc ggcgttcgat
     5821 ctttcggccc ctggcgctgc tcggggacga gcgtttccgg caacagccat cgcaaccccg
     5881 gcagcaacac catgggcagc actccgccaa tgatgaacca cgtgcgccag tcgaagtccg
     5941 gcgggaaaac ctgggtgagc agcgccacgg tgccgccgcc gatcggcagg ccgcagaaca
     6001 ccagcgaagc cgtcagagct cgtcgcgcgg gtgagctcaa ttccgcggca atggccatca
     6061 tgttgggcag cgccgcgccg aaccccagtc ccgcgaggaa gcgcgctgtc accaacgcat
     6121 cgtaactctt cgcgatggcg acccagaacg tgaacaggcc gaacaaggcg acggcgctca
     6181 gcagcacggg cctgcggccg gtgcgatcgg cgagccagcc gccaaccgcg gcgcccagca
     6241 ccatgccgat gttgttcacg gaaaacagcc agcccttggc atccgcgtcg agaccgaact
     6301 ccggaccaag ccgcggcatg gcgacgccca tcgcctggat atcgaagcct tcgagcacgg
     6361 ccacggccat gcacagcgcg atcgccacgg cattacctgg tttcacacac tccccttaca
     6421 gcgcaagccc gacgtaattc tccgccatgc tggcctgcgc cgcgcgggat tcgaacaggt
     6481 gctcgagctc ggcgtgttgc agccgttcgt cgaacgcgtc gccgggaaag cggtgaagct
     6541 gccgggtcat ccaccacgag aaccgctccg cgtgccagat gcgcctgagc gcacgcgccg
     6601 agtaggcgtc gagagcgctc tcggcgcggt caccaggagg ctcgcgatag aagtcgcgta
     6661 gcgcctcgga tagatagaac acatcgctca cggcgagatt gagccccttg gcgccggtcg
     6721 gtggcacgat atgcgccgca tcgccggcca ggaacagcct gccgaaacgc agcggctcgg
     6781 ccacgaagct gcgcagcggc gcgatgcttt tttcaataga aggcgcaatc ggagtgctcg
     6841 gcacgcgcgc gccgatctcg ctgcccaggc gccgcccgaa ctccgtccag aagcgctcgt
     6901 cgctccattg cccgatgcgg tcttccagcg agcactgcag gtagcagcgc acacgtgtgg
     6961 gcgagcgcat gctcgcaagc gcaaaaccgt gctccgaatt ggcgtatatg agctcctccc
     7021 acggcggagg gaaatcggcg agcacgccaa gccacccgaa gggatagacc cgctcgtaca
     7081 cgctcagcgc agccgctgga atgctgcgcc ggctgatgcc gtgaaaccca tcgcagccgg
     7141 cgacgaaatc gcacgccacc tcgtggccca cgccccggtg acgatacgtc actcgcgggg
     7201 acggtcccgc gatgtcgtgc agcgcgacgt cctcggcttc atagacgctg ggcgcgccgg
     7261 ccgcagcgcg cgcgtccatg aggtcgcgcg tcacctcggt ctggccatac accgtgacac
     7321 tcttgcccgt gagcgccttg aggtcgatgc gatgccggcg gccacgaaag ccgatctcca
     7381 cgccttcgtg caaaagcccc tcggcatgca gccggcttgc cacgcctgcg cgctcgagca
     7441 cgttcacggt gcccggctcg agtacgcctg cgcggatgcg gccggataca tactccgcgc
     7501 tgcgccgttc caacaagatt gtttcgattc cctccttgta caacagctgg cctagcagca
     7561 gccccgcggg tcccgcgccc acgatggcaa cctgggttcg ccgcatcgtc agaactcgaa
     7621 cttcagcgag gcaaaccact ccagcggccg tgagtacgcg acctccgcgt agccgctgcc
     7681 cgtggtcaat gaagagttgc ccgcaatggg atattcctcg tcggtgaggt tgttcacgcc
     7741 cagcgtgacg ctccacgggg tgtcgtcacc gccgccgaag gccaccgacg cattcaccgt
     7801 cagcacgtca tcgagctgtg cgatctcggg tgtgttggtg gcgtcgaaga aggtggtcgg
     7861 ctggaaagac gcatcgacac gcggcttgat ttcaagcgaa ccggcgtgcc cggtgtacgc
     7921 aatgcccagg tgcgcctgcc attccggcgc gaacggcagg aaattcccct cgacgaggcc
     7981 cggcggcagg accgccagcg gaatattcga caggctgtcg atcgtcgcgt ccagataacc
     8041 cacgctcgcg tcgatcagca ggttgtccgt ggccgcccac gtgaattccg cttcggcgcc
     8101 atcgatgctc gtttcgcccg cattggtgat gaagggagcc acgccgttcg gggccggtcc
     8161 gcgatacgtg agctgcatgt tggtgtagtc ggaactgaac accgcgaggt tcaggcgcat
     8221 cgtgtcgttg gcgagatcga attttgcgcc cagctcgatg gtctgcgcct cttcctgatc
     8281 gaacttctgc agcgccgcct gctgcgtcgg agtgagtacg gcattgaagt ggctgttcca
     8341 gccgccaccc ttgaaacctt ccgaataact caggtaggtc atggcgcttt cattccactc
     8401 gacggagaac gccgcggacg gcgtgaacga cgtgaacgtc tcgcggtacc actgcacggg
     8461 cacctgcttg atgttgggcg tcgcgtaatc gaactgatcg ggatatgaac ccttgtcgtc
     8521 ttccgtgtat cggccgccca cggtgagcgc gaacttgccg gcgaaattgt acgtccactg
     8581 cgtgaacgcg gcccagctcg agttgtcgac ttcgttgttg tcgctgtcac gctgcacccc
     8641 cggaggcggc ggattcaggg cgatcgtcgc gatgtcgtcg gaggtctgct cgaagtagta
     8701 gaggcccacg acgccgacca gcggatcggt ctcgtagacg aactgcagtt cctcgctcaa
     8761 ctgatcgccg ctcacgtcgt actcggtatg cagaatcgtg agcggagtgt tgtcggcgtc
     8821 gcgcgcgccg gtccaatccg atttgcgata cgcggtgatc gacttgagcg tgatcgactc
     8881 gctgagatcg aacgcgatgt tgaacgaggc gcccacgttg tccagcgtgc tgtagagcgg
     8941 cagcgtgccg ttgttgctgt acgggccggc gccctgcaga tcgttggcgc accgggggtc
     9001 gctgatcatc ggcacctgcg gctggtccgg aagacctggt ggcggcactg caccgtcggt
     9061 gaggcccggg cacccggcgt ccaggctcgc gacgcgcggg aaggtcgcag cttcgttgat
     9121 ggttgcgaac accagcgggc tgccattttc gtctgaactg gtcacgtcga cgatgaaggt
     9181 ggcacgcacg cgctccgacg gcgtccagac tcccttgaac atgccgaaga acgtgtcggt
     9241 gtcgcccagg tccgtgccgt cgggacgcgt gacgtagccg tcctgcttgc gcatgcccag
     9301 agagagcctg gtcttgaatt tttcaccgaa cggaatatcg agcgagacct gtccgtccgc
     9361 gaggctgtcg ttgccgagtt ctgcacgcac ttcaccgccg aagtcgtctc ccggatccgt
     9421 ggtcgagatg agtactgcgc caccgatcgt gttgcgaccg aacaaggtcc cctgcggacc
     9481 gcgcagaacc tgcacattgg cgacgtctcg cagcgccatc gaggcgccaa cggcattgcc
     9541 gatgtagacg tcgtcgatgt aaaggccaac gcccggatcg acggtggatg tcggatccgt
     9601 ctggccgatg ccgcgaatga acacctgcga cgaagagttg ttccccgcga gcggcgcatt
     9661 gttggcgaac tgcaggttcg gcaccacctg gtccagcacg tcggtgcgga acacctgccg
     9721 ctcctcgagc gagtcgcccg agagcgcggt gatcgcgatc ggcgtcttct ggaggtcttc
     9781 ctccaccttg cgggccgtga cgatgatttc ctcgaggcgc tgcacttcag gcgcctgcgc
     9841 aattgccgag ggggcgtatg ccgtggctgc cgccatggcg atcgcctggg tgaccgccat
     9901 tgccagtggc ttggcttgtg gtttggtttt catggttctc cctgggtatg tgttacttcc
     9961 agacttcacg cgcggtctcg atgacgagcg cgagcttggc cttctgttca tccaacgaga
    10021 gcgcgttgcc ctcttccgtc gacgagaagc cgcattgcgg actcaagcag agctgctcga
    10081 gtgggacgaa tcgcgccgcc tcgtcgatgc ggcgcttgag gtcatctttg gtttcgagct
    10141 ggccacgctt cgtcgtcacg aggccgagca cgacaaattt tccttttggt acgaagcgca
    10201 acggctcgaa gccacccgaa cgcgcgtcgt cgtattcgag aaagaagccg tcgacggcga
    10261 gctcgttgaa cagcgcctcg gccacgtggt cgtatccacc ctcggcggcc caggccgaac
    10321 ggaaattgcc gcggcacatg tgcgtgcaca cccgcatgcc tgccggcttc cccgccagcg
    10381 ccgcattgat gttgcggata tagatgcgat gctgttcgtt cggatcgcca ccgatgctcg
    10441 acacgtactc gcgttgcttc ggatcattga gatacgcgag gctcgtgtca tcgagctgca
    10501 gataggtgca gccgcgcttc gcgagttcgg cgatctcccg cgcatagacg gcggaaagat
    10561 cccgccagaa ttcctccatg cgtggataga ccgtctcgtc gatggcggcg cgcccaccgc
    10621 ggtagtgcat catgctgggc gagggaatcg tgagcttcgg agtgccacgc ttcaccaccg
    10681 atttcaggaa atcgaagtga tcgccgaaga tgcagccgtc gagcgacaac ttgccttcca
    10741 ccttgagccc cgagggagtg aagtccacat cgcccttgtc gctatggaaa tgcacggtga
    10801 tgttctgctg aaccttgcgc acgccgccga tctggtacag aaaatccatg tgccacgacg
    10861 agcggcggaa ttcaccgtcg gtgatgcctt gcagtcccaa cgcttcctga acctgcacca
    10921 catcgcggat cgcagcgtct tcggccttgc gcaggccggc ggcatccagc cgccccgcct
    10981 tgtgctccgc gcgcgcgcgg gtgagctcgg gaggccgcag caggctgccc acgtggtcgg
    11041 ctcggaatgg cggttgagtc agcggcatag ttgctgttcc agttcgtgga gggtgcggta
    11101 gcaaggcagc acctgtgcca cgctcgaatt cggttcacga ccatcgcgga tggcggcaat
    11161 gaactcgcgg tcctgcagtt cgatgccgtt gagcgagacg tccacttttg acacatcgat
    11221 cgcgttgttc ttgccgtcga cgagttcgtc gtagcgtgcg atgtaagttc cgtggtcgca
    11281 gatgtaacgg aaaaacgtac ccagcgggcc gtcattgttg aaggacagcg acagcgtgca
    11341 gatggcgccg ctggccgcct gcaactggat agacatgtcc atcgcgatac cgagcaccgg
    11401 gtggataggc ccctgcacga cgttggcccg cacgatttcc gagcgcgcct ggtatcgaaa
    11461 caggtccacg gtatgcgccg cgtggtgcca tagcaggtga tcggtccatg aacgcggctg
    11521 ccccagcgcg ttcatgttgc tgcgacggaa gaaatacgtc tgcacgtcca tctgctggac
    11581 ggccagttcg ccagcttcga tgcgccggtg aatccactga tgcgaaggat tgaaacgccg
    11641 cgtgtggccg accatgcaca cgagccgcgt acgttgcgcg agctccgcaa ccgcgatcgc
    11701 gtccttcagc gaatccgcga gcgggatttc cacctacacg tgtttgcccg cgttcaggca
    11761 ctgcacgctt tgcgccgcgt gcatctgtgt cggagtggcg agaatgacgg cgtcgactcc
    11821 cggacgggcc agtgcctcgg cgagatccgt ggtcgcatgc gcaacgccgt acttgcgcgc
    11881 tacctcttgc gtgggcgcga gctggcgtcc caccacggat acgacctcga cgccctcgat
    11941 caatttgcag gcatccagat gcttctggcc aaaagccccg acgccggcga cgacaacctt
    12001 catggctttc atgccagcgg ctccacgatg agatgcccga cagccgtatt cgacgcgggc
    12061 acgtgataga agcgatggat ttcgcgcacc gggccttcga gagcgccgcg catgatgagc
    12121 cacatcacga gctcgatgcc ttcggaaccg gcctcacgca agtattgttc gcgagga
//
LOCUS       DASQJS010000473        12673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U11892
            k141_626887, whole genome shotgun sequence.
ACCESSION   DASQJS010000473 DASQJS010000000
VERSION     DASQJS010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945615
            Sequence Read Archive: SRR6958124
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 08:56:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,729
            CDSs (total)                      :: 3,694
            Genes (coding)                    :: 3,680
            CDSs (with protein)               :: 3,680
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 14 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12673
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626887"
                     /isolate="SMAG_U11892"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.0262 N 109.5407 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..636
                     /locus_tag="VEW08_18600"
     CDS             172..636
                     /locus_tag="VEW08_18600"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HYJ42807.1"
                     /translation="MREFLQRRLVAPLLELLRQGVTPRELALCLALGTTIGLIPVLGV
                     STALCALVALLLKLNMPAIQLVNYLLTPLQLLLVIPLLRLGETLTGSPPFPVTLESAL
                     ALLSQDAIHAIRILATAIAHATLAWLLVAPLLAIALYRGLEPVLMHLRRDAR"
     gene            633..1685
                     /locus_tag="VEW08_18605"
     CDS             633..1685
                     /locus_tag="VEW08_18605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYJ42808.1"
                     /translation="MTSLDMRLLERDLVPDFLIRRRIRALLSARLHEEDQCDPEKQQQ
                     RLAGFIRTLHQGPVAIETAAANEQHYEVPTEFYERVLGPHLKYSSGYFSHPGAAPAEN
                     LEAAEARMLALTCQRARIDDGQKILELGCGWGSLSLWMAANYPGSRITAVTNSRTQKR
                     YIDAEARARNLANLEVTTRDVNHLAFPEGTQFDRVVSVEMFEHMRNYDMLLARVASWM
                     APAATLFVHIFTHRQYAYAFEVRDETDWMARHFFTGGIMPSDDLLLYFQRDVRIVEHW
                     QVPGWHYQLTSEAWLSNMDRHRAELMPVLARTYGEPEALRWWVYWRVFFMACAELWGY
                     ADGREWLVSHYLFERR"
     gene            1707..3128
                     /locus_tag="VEW08_18610"
     CDS             1707..3128
                     /locus_tag="VEW08_18610"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYJ42809.1"
                     /translation="MTAVKFSGWLITATLACVTCLWAAPAYAVTDSDRVAVYKEFRSQ
                     FDARKYAEAQPLAERLVALTEEQYGAEDLALTNPLTNLATVHYKLGHYPVAIENYQRT
                     LRILQAKSTIADKQQIRPLHGLGVSFMGARDPESAVVALKRAADLSRNTDGLFNINQV
                     EFIDDLIDAYESTGRFVEAEKESLYAMRVEEAAYGRNSIKLLNRLDKLALWYEGARRY
                     TSERNIYERALAILAKSAPVNDLRRVAPLRGIARSFRLEAFYGAEGADTGASFNAGTS
                     GAPVFSDGTVQRRGETSLITALDIINGNTPLNEQMRGEVLTDLGDWFLISNALRRAYD
                     TYADAWKALASVSNTKALDAPRVLAYRPSISSIDRSQLDPAEAVIKIVELHFKVDRDG
                     RIDEVTSPTTDVPENIVRNSMSSMKRSRYAPRIENGVAVPTDNVVFYERVMVKVTSPG
                     TETEKKPEAPATPAESSPPPAGR"
     gene            complement(3037..3822)
                     /locus_tag="VEW08_18615"
     CDS             complement(3037..3822)
                     /locus_tag="VEW08_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42810.1"
                     /translation="MKVRRISKLCIPVASLTLMLGGCAAVAVRPEGDLPKPLIVPTPA
                     KVGVVVTAEAGNYIHKESRASVNYEAQLGPSHRHMIEEVFRAEFTEAKMFDTVDAARL
                     EGGLMAIFEPRIEQFSFASAKETGGVYCAVTIRYQIFIYAPNGELVDTLTLTGYGSGP
                     APKIGNGEEELGIAAYAAMRDAAAKFLTQFQGLELAKPLLASQALTPKSTPAPGSPEA
                     AAAVADMTIEAVPINDQPVVATTPPESQAPPVSSPSQFRATSL"
     gene            complement(3861..4916)
                     /locus_tag="VEW08_18620"
     CDS             complement(3861..4916)
                     /locus_tag="VEW08_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42811.1"
                     /translation="MALGKRREFEVFTLSFLDCICCGFGAIILLLVLTDIGQPIVIER
                     SEKDLRGQILELQRQLFELRGETDVLNRELQGRMKVLTVEQQKAAALAGDLSKIRGEF
                     NASKGESAVTNIVETELVAAHQTLTAEMQRLLKNARPRDPNVLAVGGIPVDSEWIIFV
                     IDTSGSMQADNWENAQNVMKEILDIYPKVKGLQILDDQGKPMFASTKGQWLSDTPAQR
                     AKILSTMVNWKPFSDSSPVEGIGAAVQGWWAADKKISVYVIGDDFTGPSVEAALQAVR
                     KFNPVDRQGRHKMRIHAIGMPAGASSPPRINQRFSVLMRAMCDENEGTFVGLTIGGPG
                     CAIKVDVFGAPRCITDG"
     gene            complement(4926..6020)
                     /locus_tag="VEW08_18625"
     CDS             complement(4926..6020)
                     /locus_tag="VEW08_18625"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HYJ42812.1"
                     /translation="MKKRRAFEVFTLSFLDCICCGFGAVVLFYTIVQAQKGAQEILAL
                     DQLTGEVSKLEEEVKEGTLHLVQLRNTLEKTDEDAAAAQARALRVAAELKQRREQSST
                     YDEESLARRAHINQLKADIKSLEEGVKRLQGGALDKAPAGERVKSFRGRGNRKYISSL
                     QMKGKRILILLDTSASMMDEDIAKVIKLRNQPEPLRRSAQKWRRALDIVEWVTAQLPD
                     NGQFQVIGFNSKAKPVQANTGTQWLSNNDPRAINGILTAARAITPGDGTSLVNAFLSV
                     RTMIPEPDQIILITDGLPTQGSVPAARKFINSRDREKLFDDAMKIMEKKLPIDVVLLP
                     MKGDPLAAHAYWRLARRSSGSYVIPSRDWP"
     gene            complement(6017..7375)
                     /locus_tag="VEW08_18630"
     CDS             complement(6017..7375)
                     /locus_tag="VEW08_18630"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HYJ42813.1"
                     /translation="MYGFHFDHHTLSIARDGELLATEPLAVETSASDPRFGRAALAAA
                     RGRPDEVSLSHWRGLTTDANAARNVALEVHAHLRALSVTERIEAVVAVPADLDASALA
                     VLRGALSAAGVDTRDFLDSATLTAAAVVDRSHYVLLDAGWRSATATRVAGGAECIFEE
                     AFVSERANLLDVYDLWLIAVAGALVKNTRFDPLLSLDVEQRLFADLPMLAARAATEGK
                     VEAVVESEGARFAVPVDAQLFADAAQPFYRELTRLTRNARIAGQDAALVLPAESRRWP
                     GFLPRLLELEQDGLVIAPAGIAAVAASLQSGDPAAAPRLRRRVARVSEHSLSANIEYV
                     ASASTSAARALPVTHILFGGDSMRFPDTGLVIGTEEVDNAPHIALPRAAAGVSRRHCS
                     LRREGERTVLIDHSRYGTWVNGARVRERAAVRPGDRVRVGTPGVEFTLISAAASVREN
                     AS"
     gene            complement(7403..7792)
                     /locus_tag="VEW08_18635"
     CDS             complement(7403..7792)
                     /locus_tag="VEW08_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYJ42814.1"
                     /translation="MRHLVLTIVAISIAGVAAIAATTALLNAPLRIGPATSSTPAPAQ
                     PGQTQSPAEGGAKPTSQNPDAPPPKGAEPQPSTPATADPAGAKPPAEGETPDAPDEDP
                     YEGIAPEDLPPDLQYDADASVSFPTNT"
     gene            complement(7802..10243)
                     /locus_tag="VEW08_18640"
     CDS             complement(7802..10243)
                     /locus_tag="VEW08_18640"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HYJ42815.1"
                     /translation="MEPSLSYDASIVLRTPQDEVRYGARVVFDAGSGALRSDHDDAPP
                     PEHQPLASLRAVSGRWLFDASPESRVSINGVTVGGARIISAGDVLTIAGSQLLVEEAT
                     SSTLALRRFDLVGNDTLPPVGDSVRVLAPSIEDLPIDLGELPDLEGFVAPPARTVTGS
                     KWNYAAWVMGGLLAVVLSLFALLEPIALDLQPGDATVTSEGSFSWQSASSVFVFPGEH
                     RLHASREGYQPAEAQVTVGGPAPAHALIHLVKLPGVLQVDTGGVTAEISADGAPLGRV
                     PGAVKVPAGDRTLTFKAPRHLDHVERLTIAGGGEKQQLKVALKPSFGVVGISSVPAGA
                     QIEVDGKAVGVTPAKIEMDAGIRRVQVSAPGLRIWTSSVVVNAGTPQTIGPIELGAAD
                     ARVTVRSVPSGAQVTTGGSFRGITPVTIELSPGVSHAVSVTRAGYAPWTREIFAEAAK
                     ESTLDARLAALLVEVRIQGDPADAEVFVNGNPQGKAPVSLSLPASRQHVEVRKEGFKA
                     FVTDLVLAPGIARTVDFKLVNPKDIAGNSPQKIVTKPGIRLIIVAGGVFQAGTDRREQ
                     GRRPNEGSHKVTLLRPFYMGEREITNAQFRLFRSNHNSGAAGDHSLDLDKQAVSRVTW
                     EDAAEFCNWLSTEEGLPPAYEPLAGGGFALTVPVSNGYRLPTEAEWEFVARAASTGKP
                     LKYPWGAELPLVSGSANIGGSEAVNLLGAVIEGHKDEFPVAAAPALFAPNALGFYDFA
                     GNVSEWVNDRYLSFVASTPVTDPLGPSEGKNHTYRGSNWRTVSTGELRFPWREGAVVA
                     SDVIGFRVARYVAPE"
     gene            complement(10265..12649)
                     /locus_tag="VEW08_18645"
     CDS             complement(10265..12649)
                     /locus_tag="VEW08_18645"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYJ42816.1"
                     /translation="MTNQNQAGTKLCGRFVLIEKLGAGGYGEVWRARDEMRDTEVALK
                     ILYSQFLNSDAAWEVFQREFTLTARLNHPGVLRVYEPVRAADATVLPMVFAAGGDLRQ
                     MRGAPYTKILPLLIDIAAALEHAHSRRIVHRDLKPSNILLDAAGRPLLADFGAGASEG
                     DNSSGPPGSPFSASPQQLSGEPARPTDDIYGLGALAYELLSGYPPFYPAFDAKKIATE
                     PVPRLKPVKPIPPRLELLVSWLLAKQGRERPPSMRHVADEMNAVLQDTLGLEGAPLDV
                     AAESEAAILAASADILTPAVMADETPTPASSVRELEEIDPGMLPQVDELRAQVIREQR
                     ARNRRRSWAIAAVLIIVAGGVAVLLPKFASEQKVDLADLQLPAGPTEADQVAEKRVEE
                     LTTRHTELSKRLLELDSHAAAQWGGTDFAAARKSLDEIGGMLERRKYESAPAPLAELE
                     KALAAIEAKQPEALTAQLTEGKRALNAGEFENARQAFDTALKIDPQNQEATEWLGKVA
                     AASGVVPTLADAENAEAASDLPKAQALFTDVLKRNPGNVAATEGLARVKHAVAEKQFN
                     AEMGAGLAALNGGRLSEARTHLERASQLRPDSAEVRAALQRVADTGSGRSTAELAERA
                     SSLAAEERWSEALAIYDEALARDPSLQFAQKGRAAVAPRAELGKRLQTLIERPERLAA
                     DEVRVEAERLLVRARALPSQGPVIRSQITRLELLLPTFNQPVVLALESDNATEVAIQR
                     VGLFGAFDRREVTLKPGKYTVTGKRSGFRDVRREITVSPGQGGQTIVIRCLEPI"
BASE COUNT         2081 a         4142 c         4182 g         2268 t
ORIGIN      
        1 tacgcgtggg tcctgtggaa ggaggtgcgc gccctgccgc gcgcgttgtg gttcgtcgcc
       61 atcatgcttc tcggcaacat tgcgatgtcg gcctacgtgc tgcgccagct cgcgcgtctg
      121 agcccgaagg accccgtgga aacactgctc tcggcgcgaa attcctgaca catgcgcgaa
      181 ttcctgcaac gacggctcgt cgctccgctg ctcgagctcc tgcgccaggg ggtgacgcca
      241 cgcgagctcg cgttgtgcct ggcgctcggc acgacgattg gcctcattcc cgtgctcggg
      301 gtatcgaccg cactgtgcgc actggtggcg ctgttgctca agctgaacat gccggcgata
      361 cagctcgtca actatctact cactccgctg cagctcctgc tcgtgatccc gctgctgcgc
      421 ctcggcgaaa cgctcacggg ctcaccgccc tttccggtga cgctcgaatc ggcactggcg
      481 ctgctctcgc aggacgccat ccacgccatc cggatcctcg cgacggcgat cgcgcacgcc
      541 accctcgcgt ggttgctggt cgcgccactg ctggccatcg ccctgtatcg cgggcttgaa
      601 ccagtcctga tgcacctgcg gcgggatgcc cgatgacttc gctcgacatg cgcctgctgg
      661 agcgcgacct ggttccggac tttctcattc gccggcgtat tcgcgccctg ctgtccgcgc
      721 gacttcacga ggaagatcag tgcgaccccg aaaagcagca gcaaaggctg gccggcttca
      781 tccggacact ccaccagggc ccggtcgcca tcgaaaccgc ggcggccaac gagcaacact
      841 acgaggtgcc cacggagttc tacgagcgcg tcctgggtcc gcacctcaag tactcgagcg
      901 gctatttttc gcacccaggc gctgcaccgg ccgaaaacct ggaagccgcg gaagcccgca
      961 tgctggcgct gacctgccag cgcgcccgga tcgacgacgg ccagaaaatc ctcgaactgg
     1021 gttgcggctg gggcagtctc agcctgtgga tggctgctaa ctaccctgga tcgcggatca
     1081 ccgcggtcac caactcgcgc acgcagaaaa ggtacattga cgccgaggcg cgtgcccgaa
     1141 acctggcgaa cctggaggtc accactcgcg acgtcaacca cctcgcgttc ccggaaggca
     1201 cgcaattcga tcgcgtggtc tcggtggaga tgttcgagca catgcgcaac tacgacatgc
     1261 tgctcgcacg cgtggcctcg tggatggcgc cagccgccac acttttcgta cacatcttca
     1321 cgcaccggca atacgcctat gcgttcgagg ttcgcgatga gaccgactgg atggcgcgtc
     1381 acttcttcac cggcggcatc atgccgagcg acgatctgtt gctgtacttc cagcgggacg
     1441 tacggattgt cgagcactgg caggtgccgg gctggcacta ccagctcacc agcgaagcct
     1501 ggctttcgaa catggaccgc catcgggccg agctcatgcc ggtgctggcg cgcacctatg
     1561 gcgagcccga agcactgcgc tggtgggtgt actggcgcgt gtttttcatg gcctgcgcgg
     1621 agctgtgggg atacgccgac ggccgcgaat ggctggtatc gcattacctt tttgagaggc
     1681 gctgaaaagc ggtacagtcc catgccatga ctgccgtcaa attctcgggg tggctgatca
     1741 ccgccaccct cgcctgcgtc acttgcctgt gggcggcgcc cgcgtatgcg gtcaccgatt
     1801 ccgaccgagt cgccgtctac aaggaattcc gctcgcagtt cgacgcccgg aaatacgccg
     1861 aggcccagcc cctggccgaa cgtctcgtgg cgctcaccga agagcagtac ggtgccgaag
     1921 acctggccct gaccaacccg ctcaccaacc tggccacggt tcactacaag ctcggccact
     1981 atccggtggc catcgaaaat taccagcgca ccctgcgcat cctgcaggcc aagtcgacca
     2041 tcgccgacaa gcagcagatc cggccgctgc acggcctggg cgtcagtttc atgggcgcaa
     2101 gagatccgga atccgcggtg gtggccttga agcgcgccgc cgacctgtcg cgaaataccg
     2161 atggcctgtt caacatcaac caggtggagt tcatcgacga tctgatcgac gcgtacgaat
     2221 ccaccggcag gttcgtcgag gcggagaagg aaagcctgta cgccatgcgc gtcgaagagg
     2281 ccgcctacgg ccgcaattcg atcaagctgc tcaatcggct cgacaagctg gcgctctggt
     2341 atgaaggcgc gcggcgttat accagtgaac gcaacatcta cgagcgcgca ctggccattc
     2401 tcgccaagtc agcgcccgtg aacgacctgc gacgggttgc tccgctgcgc ggcatcgcgc
     2461 gatctttccg gctcgaggcc ttctacggcg ccgaaggcgc ggacaccggc gcttccttca
     2521 acgccggaac ttccggcgcg ccggtgttct ccgatggcac cgtgcagcgc cgcggcgaaa
     2581 catcgctgat caccgcgctc gacatcatca atggcaacac gcccctcaac gagcagatgc
     2641 gcggcgaagt gctcactgat ctcggcgact ggttcctgat ctcgaacgca ctgcgtcgcg
     2701 cttacgacac ctacgcggac gcgtggaaag cgctcgccag cgtgtcgaac accaaggcgc
     2761 tcgacgcgcc gcgcgtgctc gcgtaccggc cgtcgatcag ttcgatcgac cggtcgcagc
     2821 tcgacccggc cgaagccgtg atcaagatcg tggagctgca tttcaaagtt gatcgcgacg
     2881 ggcggatcga tgaagtgaca tcaccgacca ccgacgtgcc cgagaacatc gtgcgcaact
     2941 cgatgtcgtc gatgaagcgc tcccgttacg cgccgcgcat cgagaatggc gtggcggtgc
     3001 ccacggataa cgtggtcttc tacgaacgag tgatggtcaa agtgacgtcg cccggaactg
     3061 agacggagaa gaaaccggag gcgcctgcga ctccggcgga gtcgtcgcca ccaccggctg
     3121 gtcgttgata gggacggctt cgatggtcat gtcggcgacc gccgccgcgg cttcgggcga
     3181 acccggcgcc ggtgttgatt tcggtgtcag cgcctgggag gccagcagcg gcttcgccag
     3241 ttccaggccc tggaactgtg tgagaaattt tgccgccgca tcgcgcatgg ccgcgtaggc
     3301 ggcaattccc aactcttctt caccgttgcc gatcttgggc gccggaccgc tgccgtaacc
     3361 cgtgagcgtg agcgtgtcga ccagctctcc gttcggcgcg tagatgaaaa tctggtagcg
     3421 gatggtcacc gcgcaataaa cgccgccggt ttccttcgcg ctcgcgaacg agaactgctc
     3481 gatgcgcggt tcgaagatgg ccatgaggcc gccctcgagc cgcgcggcat cgaccgtgtc
     3541 gaacatcttc gcttcggtga attccgcgcg gaagacttcc tcgatcatgt gccggtgcga
     3601 cgggccgagc tgcgcctcgt agttcacgct ggcgcgggat tccttgtgga tatagttgcc
     3661 ggcttcggcg gtgaccacca cgcccacctt ggccggtgtc ggcacgatga gtggtttggg
     3721 cagatcccct tcgggccgca cggctacggc cgcgcagcca ccgagcatca gcgtcagtga
     3781 cgcgactgga atgcagagtt tggagatgcg tcgtactttc acggggcctt gtccgtcaga
     3841 aaagtcgtcc atcagaaaag tcatccgtca gttatgcagc gcggcgcacc gaacacgtcg
     3901 accttgatcg cgcagcccgg gccgccgatc gtgagaccca cgaaagtccc ttcgttttcg
     3961 tcgcacatgg cccgcattag aacagaaaaa cgctgattga tacggggcgg cgaactggcg
     4021 cccgccggca tgccgatagc gtgaattcgc atcttgtggc gcccctgccg gtccacggga
     4081 ttgaacttgc gcacggcctg cagcgccgct tccacggacg ggccggtgaa gtcgtccccg
     4141 atgacataca cgctgatctt cttgtctgcc gcccaccagc cctggaccgc ggcgccgatg
     4201 ccttccaccg ggctcgaatc gctgaacggc ttccagttga ccatcgtgga caggatcttg
     4261 gcgcgctgcg ccggcgtatc gctgagccat tggcccttgg tgctcgcgaa catgggtttt
     4321 ccctggtcgt cgagaatctg gaggcccttc accttggggt agatgtcgag aatctctttc
     4381 atgacgttct gggcgttctc ccagttgtcg gcctgcatgc tgcccgaggt gtcgatcacg
     4441 aagatgatcc actcgctatc cacgggaatg ccgccgaccg cgagcacgtt cgggtcgcgc
     4501 ggccgcgcgt tcttgagcag gcgctgcatc tccgcggtca gcgtctggtg tgccgccacg
     4561 agttctgtct cgacgatgtt ggtgacggcc gactcgccct tgctggcgtt gaattcgcca
     4621 cggattttcg acagatcacc ggcgagcgcc gccgccttct gctgctcgac ggtgagcacc
     4681 ttcatgcggc cctgcagctc gcgattgagc acgtcggttt cgccgcgcag ctcgaacaac
     4741 tggcgctgca gctcgaggat ctggccgcgc agatctttct cgctgcgctc gatgacgatg
     4801 ggctggccga tgtcggtgag caccagcagc aggatgatgg cgccgaagcc gcagcagatg
     4861 cagtcgagga acgacagcgt gaagacttcg aattcgcggc gtttgcctaa agccatttca
     4921 gtttcctacg gccagtcgcg cgatgggatg acgtacgacc cgctgctgcg ccgggcgagc
     4981 cgccaatacg catgcgccgc cagcggatcg cccttcatgg gcagcagcac gacatcgatt
     5041 ggcagtttct tttccatgat cttcatcgca tcgtcgaaca gcttttcgcg gtcgcgggaa
     5101 ttgatgaact tgcgggcggc cggcaccgag ccctgcgtgg gcaagccatc ggtgatgagg
     5161 atgatctggt cgggttccgg aatcatcgtg cgcaccgaca gaaacgcatt caccaggctc
     5221 gtgccgtcac cgggcgtgat ggcgcgtgcc gcggtgagaa tgccattgat ggcgcgcgga
     5281 tcgttgttgc tgagccattg ggtgccggtg ttcgcctgca ccggcttcgc cttggagttg
     5341 aaaccgatca cctggaactg gccattgtca ggcaactgcg ccgtgaccca ctcgacgatg
     5401 tcgagcgcgc gccgccactt ctgcgccgat cgccgcagcg gttcgggctg attgcgcagc
     5461 ttgatcacct tggcgatgtc ctcgtccatc atgctggccg aggtgtcgag caggatgagg
     5521 atgcgcttgc ccttcatctg cagcgagctg atgtacttgc ggttacctcg tccgcggaac
     5581 gacttcacgc gttcgccggc cggcgctttg tcgagcgcac cgccctgcag gcgcttcacg
     5641 ccttcttcga gcgacttgat gtcggccttg agctggttga tgtgcgcgcg gcgcgccagg
     5701 ctttcctcat cgtacgtcga cgactgctcg cggcgctgct tgagctcggc ggcaacgcgc
     5761 agcgcgcgcg cctgggcggc ggcggcgtct tcgtcggtct tctcgagcgt gttgcgcaac
     5821 tgcacgagat gcagcgtgcc ttccttcact tcctcttcga gcttgctcac ctcgccggtg
     5881 agctggtcca gcgccaggat ttcctgcgcg cctttctgcg cctgcacgat ggtgtagaac
     5941 agcaccaccg cgccgaagcc gcagcagatg cagtcgagga acgacagcgt gaaaacttcg
     6001 aaggcgcggc gttttttcac gacgcgttct cgcggacgga tgccgccgcg gaaatgagag
     6061 tgaactccac gcccggcgtg ccgacgcgta cccgatcgcc ggggcgcacc gcggcacgct
     6121 cgcgcacgcg cgcgccgttc acccaggtgc cataacggct gtggtcgatg agtaccgtgc
     6181 gctcgccctc gcgacgcaac gaacaatggc ggcgcgacac tcccgccgcg gcgcggggca
     6241 gtgcgatgtg cggagcgttg tcgacttcct cggtgccaat caccaggccc gtatccggga
     6301 agcgcatgga atccccgccg aagaggatgt gcgtgaccgg cagcgcgcgc gcagcggacg
     6361 tgctggccga ggcgacatac tcgatgttcg cggacaggct gtgctccgag acgcgcgcaa
     6421 cgcgacggcg aagacgcgga gcggcggccg gatcaccgct ttgtaacgaa gccgcgaccg
     6481 cggctattcc ggcgggcgcg atgaccagcc cgtcttgctc gagctcgagc agccggggca
     6541 ggaagcccgg ccagcgtcgc gactcggccg gcaaaaccag cgccgcatcc tgtccggcga
     6601 tgcgtgcatt gcgcgtcagg cgcgtgagtt cgcgatagaa cggttgtgcc gcatcggcga
     6661 agagctgggc atcgaccggg acagcgaagc gtgcgccttc ggattcgacc acggcttcca
     6721 ccttgccctc ggtggccgcg cgcgcggcga gcatgggcag gtcggcgaac aagcgctgtt
     6781 cgacgtccag actgagcagc ggatcgaagc gcgtgttttt tacgagcgcg ccggccacgg
     6841 cgatgagcca cagatcgtag acgtcgagca gattggcacg ctcgctcacg aatgcttcct
     6901 cgaaaatgca ctcagcgccg ccggccacgc gcgtggcggt ggcggagcgc cagccggcat
     6961 cgagcagcac gtagtggctg cggtcgacca cggccgcggc ggtgagcgtg gcggagtcga
     7021 ggaagtcgcg cgtatccaca cccgcggcac tcagcgcacc gcgcaggacc gcgagcgcgc
     7081 tcgcatccag gtcggcgggc acggcaacca ccgcttcgat gcgctcggtc accgagagcg
     7141 cacgcaggtg cgcatgcact tcgagcgcga cgttgcgcgc cgcgttggcg tcggtggtaa
     7201 gcccacgcca atgcgaaagc gatacttcgt ccgggcggcc acgtgcagcc gcgagtgccg
     7261 cacgcccgaa acgcggatcg ctcgccgacg tctccaccgc cagcggctcg gtggcgagca
     7321 actcgccatc tcgggcgatg gagagcgtgt ggtggtcgaa gtgaaagccg tacatataag
     7381 agtgggtcag gccggtttca gattacgtat tcgtcgggaa gctgacgctc gcatcggcgt
     7441 cgtattgcag atcgggcggc agatcttcgg gggcaatgcc ttcgtaggga tcctcgtcag
     7501 gagcatccgg agtttcgcct tcggctggcg gtttggcgcc cgcgggatcg gcagtggcgg
     7561 gcgtggaggg ttgaggctca gcgcctttgg gtggcggcgc gtcggggttt tgggaggtgg
     7621 gcttcgcgcc accttcggcc ggcgactgag tctgaccggg ttgcgccggg gccggcgtgc
     7681 tgctcgtggc gggtccgatg cgcagcggcg cgttcagcag agccgtggtg gcggcaatcg
     7741 cggcgacgcc cgcaatgctg atggcgacga tcgtgagaac gaggtgacgc atgtcgctct
     7801 cctattccgg tgctacgtag cgcgcgacac gaaagccgat cacgtcactg gcgacgaccg
     7861 cgccttcgcg ccaggggaag cgcaactcgc cggtggacac cgtccgccag tttgaaccgc
     7921 gatacgtatg attcttgcct tcgctgggac cgagcggatc cgtgacggga gttgaggcca
     7981 cgaatgacag gtagcggtcg ttgacccatt ccgacacgtt gcccgcgaag tcgtagaaac
     8041 cgagcgcatt cggcgcgaac agcgccggtg cggctgcgac cgggaattcg tccttgtgtc
     8101 cctcgatgac cgcgcctaac aagttgaccg cttcgctgcc gccgatgttc gccgagcccg
     8161 acaccagggg cagttcggcg ccccagggat acttgagcgg cttgccggtg ctcgcggcgc
     8221 gcgcgacgaa ctcccactcc gcctccgtgg gcaggcggta accgttgctc accggaaccg
     8281 tgagtgcgaa gccaccgccg gcgagtggtt cgtaagccgg tggcaggccc tcctcggtcg
     8341 agagccagtt gcagaattcc gcggcgtctt cccaggtgac gcgcgacacg gcctgcttgt
     8401 cgagatcgag cgagtgatcg cccgcggcgc cggaattgtg gttggagcgg aacagtcgga
     8461 actgcgcgtt ggtgatctcg cgttcgccca tgtagaacgg gcgcagcagc gtcaccttgt
     8521 gggaaccttc gttcggtcgg cggccctgtt cgcggcgatc cgtgcccgcc tggaacacgc
     8581 cgcccgcgac gatgatgagc cggataccag gcttggtgac tatcttctgc ggcgaattgc
     8641 cggcaatgtc tttcggattc accagtttga aatccacggt gcgcgcaatg ccgggggcca
     8701 gcaccaggtc cgtgacgaac gccttgaaac cttccttgcg cacttcgacg tgttgacggc
     8761 tggcggggag cgacagcgac accggcgcct tgccctgtgg attgccattc acgaaaactt
     8821 ccgcgtcggc cgggtcaccc tggatgcgca cctcgaccag cagcgccgcc aggcgcgcat
     8881 cgagcgtcga ttcttttgcg gcttcggcga atatttcgcg cgtccacggc gcatagccgg
     8941 cgcgcgtgac cgaaacggcg tgcgagacgc cgggcgacaa ttcgatggtg acgggcgtga
     9001 tgccgcggaa gctgccgccg gtggtcacct gcgcacccga tggcacggag cgcacggtga
     9061 cgcgcgcgtc cgcggcgccg agttcgatgg gcccgatggt ctgcggcgtg ccggcgttca
     9121 cgacgacgct cgaggtccag atgcgaagtc ccggcgccga cacctgcacg cgccggatgc
     9181 ccgcgtccat ttctattttc gccggcgtga cgcccacggc cttgccgtcg acctcgatct
     9241 gcgcccccgc gggcaccgag ctgatgccga cgactccgaa ggaaggcttg agcgccacct
     9301 tgagctgctg tttctcgcca ccgcccgcga tggtgagacg ttcgacgtga tcgaggtgac
     9361 gcggcgcctt gaaggtgagc gtgcgatcgc cggccggcac cttgaccgcg ccgggaacgc
     9421 ggccgagcgg cgcgccatcg gcggagattt cagcggtgac gccgccggtg tccacctgga
     9481 gcacgccggg tagtttgacc aggtgaatga gcgcatgcgc cggcgcggga ccgcccaccg
     9541 tgacctgcgc ttcggcgggc tggtagccct cgcgcgatgc gtgcaggcgg tgctcgccgg
     9601 gaaacacgaa tacgctggac gccgactgcc acgaaaaact gccttcggac gtcaccgtgg
     9661 cgtcaccggg ttgcagatcg agcgcgatgg gctcgagaag cgcgaacaga ctcagcacga
     9721 ccgcgagcag gccgcccatc acccaggcgg cgtagttcca tttgctgccc gtgaccgttc
     9781 gcgcaggcgg agcgacgaag ccttcgaggt cgggcaactc gccgaggtcg atgggcaggt
     9841 cttcgatcga cggcgccagc acccgcacgc tgtcgcccac gggcggcagc gtgtcgttgc
     9901 ccacgaggtc gaagcgccgc agcgcgagag tggatgacgt cgcttcctcg accagcaatt
     9961 gcgaaccggc gatggtgagc acgtcgcccg cgctgatgat gcgggctccg ccgaccgtga
    10021 cgccattgat gctgacgcgg ctctcgggag atgcatcgaa cagccagcgc ccggacaccg
    10081 cgcgcagcga agccaggggc tggtgttcgg gcgggggcgc gtcgtcgtga tcggagcgca
    10141 gcgcgcccga gcccgcatcg aaaacgacac gcgcgccata acgcacctcg tcctgcgggg
    10201 tgcgcaggac gatcgacgcg tcgtacgaaa gcgagggttc catgactgtt tggccagtga
    10261 ggcgctaaat cggttccagg cagcggatta cgatcgtctg gccaccctgc cccggcgaca
    10321 cggtgatttc tcggcggacg tcgcgaaagc cgctgcgctt gcccgtcacc gtgtatttgc
    10381 cgggcttcaa ggtgacctcg cgccggtcga aggctccgaa gaggccgacg cgctggattg
    10441 ccacctcggt ggcgttgtcg gattcgagag ccaacactac tggctgattg aacgtgggca
    10501 acagaagttc caggcgcgtg atctgcgagc ggatcaccgg gccctgggac ggcaacgccc
    10561 gcgctcgcac cagcaacctt tccgcctcga cgcgaacctc gtcggcggcc agccgttcag
    10621 gtctttcaat gagtgtctgc aaccgcttgc ccagttccgc ccgcggagcg accgccgcgc
    10681 ggcccttctg ggcaaactgc agggaagggt ctcgcgcgag tgcttcgtcg tagatagcca
    10741 gcgcctcgct ccagcgttcc tcggcggcca ggctcgaagc ccgttcggcc agctccgcgg
    10801 tgctgcgtcc gctgcccgtg tcggcgactc gctgcagcgc ggcacgaact tcggcactgt
    10861 cgggtctgag ctggctggcg cgttcgagat gcgtgcgcgc ctccgacagg cgtccaccgt
    10921 tgagcgcggc aagtccggcg cccatctccg cattgaactg cttttccgcg acggcgtgct
    10981 tcacgcgcgc gagcccttcg gtcgccgcca cgtttccggg attccgtttc aggacgtcgg
    11041 tgaacaaggc ctgcgccttg ggcaggtcgc tggctgcttc agcgttctcg gcgtcggcca
    11101 gcgtgggcac cacgccgctg gcggccgcca ccttgccgag ccactcggtg gcttcctggt
    11161 tctgcggatc gatcttcagc gcggtgtcga acgcctggcg ggcgttttcg aattccccgg
    11221 cattcagcgc ccgcttgcct tccgtgagct gcgcagtcag tgcttccggc tgtttggcct
    11281 cgatggccgc cagtgctttt tcgagctccg cgagcggcgc cggcgcgctt tcgtatttac
    11341 gacgctcgag catgccgccg atctcgtcga gcgacttgcg ggcggcggcg aagtccgtgc
    11401 cgccccattg cgccgcggcg tgagagtcca gctccagcag tcgcttgctg agttcggtgt
    11461 gccgcgtggt gagctcttcg acgcgcttct ccgcgacctg gtccgcttcg gtgggaccgg
    11521 cgggaagctg gagatcggcg agatcgacct tctgctcact ggcgaatttc ggcagcagaa
    11581 cggcaactcc gcccgcgacg atgatgagca ccgcggcgat ggcccagctg cgacggcgat
    11641 tgcgcgcccg ctgctcgcgt atgacctgcg cccgcagttc gtcgacctgc ggcaacatgc
    11701 ccggatcgat ttcctcgagc tcgcgcaccg agctcgcggg cgtcggcgtt tcgtctgcca
    11761 tcaccgccgg cgtgagaatg tcggcggagg cggcgaggat ggccgcttcg ctctccgcgg
    11821 caacgtcgag cggagcgccc tcgagcccga gcgtatcctg cagcacggcg ttcatttcat
    11881 cggcgacgtg ccgcatcgac ggcgggcgtt cgcgcccctg cttggctaac aaccaggaca
    11941 ccagcagttc cagccgtggc ggtatcggtt tcaccggctt gagccgcggc accggttcgg
    12001 tggcgatctt cttcgcgtcg aacgcgggat aaaacggtgg atatccggag agcaattcgt
    12061 aggcgagcgc acccagcccg tagatgtcgt cggtaggtcg cgcgggttca ccggaaagct
    12121 gctgcgggct cgcggaaaac ggcgagcccg gtgggccgct ggaattgtcg ccctcggagg
    12181 cgccggcacc gaagtctgcg agcagcggcc ggccggcggc atcgagcagg atgttcgagg
    12241 gcttgagatc gcgatgcacg atgcgccgcg agtgcgcgtg ttcgagcgcg gccgcgatgt
    12301 cgatgagcag cggcaggatc ttggtgtaag gagcgccgcg catctggcgc aggtctccgc
    12361 cggccgcgaa caccatgggt aacaccgtgg cgtcggccgc gcgcaccggt tcatagactc
    12421 gtaaaacacc gggatgattc aggcgcgccg tgagcgtgaa ctcacgctgg aacacttccc
    12481 aggcggcgtc ggaattcagg aactgggaat agaggatttt cagcgcgact tcggtgtcgc
    12541 gcatttcgtc acgggcgcgc cacacctcgc cgtacccgcc ggcgccgagt ttctctatga
    12601 gcacgaagcg gccgcacagc ttggtgccgg cctgattctg gtttgtcacg tatcagctgg
    12661 acggaggctt gac
//
